LOCUS       DADSZD010000001        72927 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.001, whole genome shotgun sequence.
ACCESSION   DADSZD010000001 DADSZD010000000
VERSION     DADSZD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 72927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.001"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            73..702
                     /locus_tag="J1Z28_000001"
     CDS             73..702
                     /locus_tag="J1Z28_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiJ family protein"
                     /protein_id="HBA7714878.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            729..2390
                     /locus_tag="J1Z28_000002"
     CDS             729..2390
                     /locus_tag="J1Z28_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBA7714879.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            2630..2689
                     /locus_tag="J1Z28_000003"
     CDS             2630..2689
                     /locus_tag="J1Z28_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBA7714880.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(2809..4242)
                     /gene="hldE"
                     /locus_tag="J1Z28_000004"
     CDS             complement(2809..4242)
                     /gene="hldE"
                     /locus_tag="J1Z28_000004"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBA7714881.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(4290..7130)
                     /gene="glnE"
                     /locus_tag="J1Z28_000005"
     CDS             complement(4290..7130)
                     /gene="glnE"
                     /locus_tag="J1Z28_000005"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBA7714882.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPKWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLVLVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRALLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERELVRASWQKWLVEE"
     gene            complement(7153..8454)
                     /locus_tag="J1Z28_000006"
     CDS             complement(7153..8454)
                     /locus_tag="J1Z28_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBA7714883.1"
                     /translation="MAQEIELKFIVNHSAVEALRGHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRMRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDMRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8696..9316
                     /locus_tag="J1Z28_000007"
     CDS             8696..9316
                     /locus_tag="J1Z28_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBA7714884.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            9380..10618
                     /gene="cca"
                     /locus_tag="J1Z28_000008"
     CDS             9380..10618
                     /gene="cca"
                     /locus_tag="J1Z28_000008"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBA7714885.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLSFRIADETLALM
                     REMTHAGELEHLTPERVWKETENALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEILDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVAGWKEQRCPKPE"
     gene            complement(10806..11627)
                     /gene="bacA"
                     /locus_tag="J1Z28_000009"
     CDS             complement(10806..11627)
                     /gene="bacA"
                     /locus_tag="J1Z28_000009"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA7714886.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11718..12086)
                     /gene="folB"
                     /locus_tag="J1Z28_000010"
     CDS             complement(11718..12086)
                     /gene="folB"
                     /locus_tag="J1Z28_000010"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBA7714887.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            12191..12808
                     /gene="plsY"
                     /locus_tag="J1Z28_000011"
     CDS             12191..12808
                     /gene="plsY"
                     /locus_tag="J1Z28_000011"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBA7714888.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12821..13753)
                     /gene="ttdR"
                     /locus_tag="J1Z28_000012"
                     /gene_synonym="dan"
     CDS             complement(12821..13753)
                     /gene="ttdR"
                     /locus_tag="J1Z28_000012"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HBA7714889.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            13960..14871
                     /gene="ttdA"
                     /locus_tag="J1Z28_000013"
     CDS             13960..14871
                     /gene="ttdA"
                     /locus_tag="J1Z28_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBA7714890.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            14868..15473
                     /gene="ttdB"
                     /locus_tag="J1Z28_000014"
     CDS             14868..15473
                     /gene="ttdB"
                     /locus_tag="J1Z28_000014"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBA7714891.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            15521..16984
                     /locus_tag="J1Z28_000015"
     CDS             15521..16984
                     /locus_tag="J1Z28_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBA7714892.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPILKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17027..18040)
                     /gene="tsaD"
                     /locus_tag="J1Z28_000016"
     CDS             complement(17027..18040)
                     /gene="tsaD"
                     /locus_tag="J1Z28_000016"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBA7714893.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQEALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            18278..18493
                     /gene="rpsU"
                     /locus_tag="J1Z28_000017"
     CDS             18278..18493
                     /gene="rpsU"
                     /locus_tag="J1Z28_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBA7714894.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            18604..20349
                     /gene="dnaG"
                     /locus_tag="J1Z28_000018"
     CDS             18604..20349
                     /gene="dnaG"
                     /locus_tag="J1Z28_000018"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBA7714895.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLVDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            20544..22385
                     /gene="rpoD"
                     /locus_tag="J1Z28_000019"
     CDS             20544..22385
                     /gene="rpoD"
                     /locus_tag="J1Z28_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBA7714896.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(22455..22961)
                     /gene="mug"
                     /locus_tag="J1Z28_000020"
     CDS             complement(22455..22961)
                     /gene="mug"
                     /locus_tag="J1Z28_000020"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBA7714897.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            23086..23161
                     /locus_tag="J1Z28_000021"
     tRNA            23086..23161
                     /locus_tag="J1Z28_000021"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23119..23121,aa:Ile,seq:cat)
     gene            complement(23215..23979)
                     /gene="nfeF"
                     /locus_tag="J1Z28_000022"
     CDS             complement(23215..23979)
                     /gene="nfeF"
                     /locus_tag="J1Z28_000022"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBA7714898.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHEGGVASRWAMQAQSGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            24267..24890
                     /locus_tag="J1Z28_000023"
     CDS             24267..24890
                     /locus_tag="J1Z28_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBA7714899.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGAGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(24997..26517)
                     /locus_tag="J1Z28_000024"
     CDS             complement(24997..26517)
                     /locus_tag="J1Z28_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBA7714900.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTGKRIHKGLVVRKGWLGKLPSLP
                     LRWRVRGVMTLMFIVLAAMLWFVAAPVVTYFLCALVMLLGNACFEWQIVRPIENVASQ
                     ALKVATGERNSVEHLKRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            26824..28314
                     /gene="ygjG"
                     /locus_tag="J1Z28_000025"
     CDS             26824..28314
                     /gene="ygjG"
                     /locus_tag="J1Z28_000025"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBA7714901.1"
                     /translation="MITTFVFIPIFAIAARVAQSLQYLNRYHVIREPPEHILNRLPSS
                     ASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKEHVNPGFLEYRKSVTAGGDYGA
                     VEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPVVVSAVQNQLAKQPLHSQELLD
                     PLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKLAKAYQSPRGKFTFIATSGAFH
                     GKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAMRTALNECKKTGDDVAAVILEP
                     IQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTGMGRTGKMFACEHENVQPDILC
                     LAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFGGNPLACAAALATINVLLEQNL
                     PAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEMFRQRV
                     LVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            complement(28356..28688)
                     /locus_tag="J1Z28_000026"
     CDS             complement(28356..28688)
                     /locus_tag="J1Z28_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBA7714902.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            28907..29890
                     /gene="ebgR"
                     /locus_tag="J1Z28_000027"
     CDS             28907..29890
                     /gene="ebgR"
                     /locus_tag="J1Z28_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBA7714903.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYIDQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30074..33166
                     /gene="ebgA"
                     /locus_tag="J1Z28_000028"
     CDS             30074..33166
                     /gene="ebgA"
                     /locus_tag="J1Z28_000028"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBA7714904.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLIGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLATSPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLARGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVVEQ
                     SDDEVLLVSRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLPGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            33163..33612
                     /locus_tag="J1Z28_000029"
     CDS             33163..33612
                     /locus_tag="J1Z28_000029"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBA7714905.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGLQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            33675..35108
                     /locus_tag="J1Z28_000030"
     CDS             33675..35108
                     /locus_tag="J1Z28_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7714906.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            35242..36312
                     /gene="ygjJ"
                     /locus_tag="J1Z28_000031"
     CDS             35242..36312
                     /gene="ygjJ"
                     /locus_tag="J1Z28_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HBA7714907.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLTEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            36329..38680
                     /gene="ygjK"
                     /locus_tag="J1Z28_000032"
     CDS             36329..38680
                     /gene="ygjK"
                     /locus_tag="J1Z28_000032"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBA7714908.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSMAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAIFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSTERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTDNGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            39005..41023
                     /locus_tag="J1Z28_000033"
     CDS             39005..41023
                     /locus_tag="J1Z28_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBA7714909.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPNGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGAE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAEMVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQILAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(41141..42277)
                     /gene="rlmG"
                     /locus_tag="J1Z28_000034"
     CDS             complement(41141..42277)
                     /gene="rlmG"
                     /locus_tag="J1Z28_000034"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBA7714910.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCVLAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            42362..42865
                     /locus_tag="J1Z28_000035"
     CDS             42362..42865
                     /locus_tag="J1Z28_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA7714911.1"
                     /translation="MSNLTYLQSYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            42942..43633
                     /locus_tag="J1Z28_000036"
                     /pseudo
     CDS             42942..43633
                     /locus_tag="J1Z28_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
     gene            43712..44698
                     /locus_tag="J1Z28_000037"
     CDS             43712..44698
                     /locus_tag="J1Z28_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7714912.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPTFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            44981..45946
                     /gene="alx"
                     /locus_tag="J1Z28_000038"
     CDS             44981..45946
                     /gene="alx"
                     /locus_tag="J1Z28_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBA7714913.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVE"
     gene            46270..47514
                     /gene="sstT"
                     /locus_tag="J1Z28_000039"
     CDS             46270..47514
                     /gene="sstT"
                     /locus_tag="J1Z28_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBA7714914.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILAGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(47518..48069)
                     /locus_tag="J1Z28_000040"
     CDS             complement(47518..48069)
                     /locus_tag="J1Z28_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBA7714915.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(48152..49639)
                     /locus_tag="J1Z28_000041"
     CDS             complement(48152..49639)
                     /locus_tag="J1Z28_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBA7714916.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(49654..51066)
                     /gene="uxaC"
                     /locus_tag="J1Z28_000042"
     CDS             complement(49654..51066)
                     /gene="uxaC"
                     /locus_tag="J1Z28_000042"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBA7714917.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            51549..52847
                     /locus_tag="J1Z28_000043"
     CDS             51549..52847
                     /locus_tag="J1Z28_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7714918.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            52977..53753
                     /gene="exuR"
                     /locus_tag="J1Z28_000044"
     CDS             52977..53753
                     /gene="exuR"
                     /locus_tag="J1Z28_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBA7714919.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            54098..54760
                     /locus_tag="J1Z28_000045"
     CDS             54098..54760
                     /locus_tag="J1Z28_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7714920.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            54764..55147
                     /gene="mzrA"
                     /locus_tag="J1Z28_000046"
     CDS             54764..55147
                     /gene="mzrA"
                     /locus_tag="J1Z28_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBA7714921.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            55294..55662
                     /locus_tag="J1Z28_000047"
     CDS             55294..55662
                     /locus_tag="J1Z28_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBA7714922.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            55700..56005
                     /locus_tag="J1Z28_000048"
     CDS             55700..56005
                     /locus_tag="J1Z28_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBA7714923.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            56008..56412
                     /locus_tag="J1Z28_000049"
     CDS             56008..56412
                     /locus_tag="J1Z28_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7714924.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            56402..56701
                     /locus_tag="J1Z28_000050"
     CDS             56402..56701
                     /locus_tag="J1Z28_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7714925.1"
                     /translation="MSSKVEHERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            56798..57280
                     /locus_tag="J1Z28_000051"
     CDS             56798..57280
                     /locus_tag="J1Z28_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBA7714926.1"
                     /translation="MILSIDSNDANSAPLHKKTISSLSGAVESMMKKLEDVGVLVARI
                     LMPILFITAGWGKITGYAGTQQYMEAMGVPGFMLPLVILLEFGGGLAILFGFLTRTTA
                     LFTAGFTLLTAFLFHSNFAEGMNSLMFMKNLTISGGFLLLAITGPGAFSIDRLLNKKW
                     "
     gene            57350..58336
                     /locus_tag="J1Z28_000052"
     CDS             57350..58336
                     /locus_tag="J1Z28_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBA7714927.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKERYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            58619..58987
                     /locus_tag="J1Z28_000053"
     CDS             58619..58987
                     /locus_tag="J1Z28_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA7714928.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(59021..59917)
                     /locus_tag="J1Z28_000054"
     CDS             complement(59021..59917)
                     /locus_tag="J1Z28_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7714929.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            60022..60723
                     /locus_tag="J1Z28_000055"
     CDS             60022..60723
                     /locus_tag="J1Z28_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBA7714930.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKSLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGQFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            60746..60910
                     /locus_tag="J1Z28_000056"
     CDS             60746..60910
                     /locus_tag="J1Z28_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7714931.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(61043..62353)
                     /locus_tag="J1Z28_000057"
     CDS             complement(61043..62353)
                     /locus_tag="J1Z28_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBA7714932.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(62381..63712)
                     /locus_tag="J1Z28_000058"
     CDS             complement(62381..63712)
                     /locus_tag="J1Z28_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7714933.1"
                     /translation="MEIASNKCVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(63990..65354)
                     /gene="tdcG"
                     /locus_tag="J1Z28_000059"
     CDS             complement(63990..65354)
                     /gene="tdcG"
                     /locus_tag="J1Z28_000059"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBA7714934.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKSIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     HDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(65426..65815)
                     /locus_tag="J1Z28_000060"
     CDS             complement(65426..65815)
                     /locus_tag="J1Z28_000060"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBA7714935.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(65829..68123)
                     /gene="tdcE"
                     /locus_tag="J1Z28_000061"
     CDS             complement(65829..68123)
                     /gene="tdcE"
                     /locus_tag="J1Z28_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBA7714936.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(68157..69365)
                     /gene="tdcD"
                     /locus_tag="J1Z28_000062"
     CDS             complement(68157..69365)
                     /gene="tdcD"
                     /locus_tag="J1Z28_000062"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBA7714937.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(69391..70722)
                     /gene="tdcC"
                     /locus_tag="J1Z28_000063"
     CDS             complement(69391..70722)
                     /gene="tdcC"
                     /locus_tag="J1Z28_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBA7714938.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(70744..71733)
                     /gene="tdcB"
                     /locus_tag="J1Z28_000064"
     CDS             complement(70744..71733)
                     /gene="tdcB"
                     /locus_tag="J1Z28_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBA7714939.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(71832..72770)
                     /gene="tdcA"
                     /locus_tag="J1Z28_000065"
     CDS             complement(71832..72770)
                     /gene="tdcA"
                     /locus_tag="J1Z28_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBA7714940.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVD"
BASE COUNT        17689 a        19417 c        18472 g        17349 t
ORIGIN      
        1 caaagacaaa tgtaagcaag gaaactattc caaagtaaat attgcgtcac taaatggaca
       61 ttggagtgac atatgatttt attcgccgac tataacaccc cttatctttt cgctatttcc
      121 tttgtactct tgatcggcct tttagaaatt tttgcactta tctgcggtca catgctctcc
      181 ggtgctcttg atgcacatct tgaccattat gattccataa ccacaggtca tattagccag
      241 gcacttcatt atctcaatat tggaagatta ccagctctcg tcgtcctctg cttactcgct
      301 ggtttttttg gtcttattgg catcctgttg caacatgcct gcgttacgct ctggcaatca
      361 ccactcccca acctgttcgt ggttcctgtc agtttactgt ttacaattat tgcagtgcat
      421 tataccggta aagtcgttgc accctggatt ccacgcgacc atagttcagc tattacagaa
      481 gaagaatatg ttggtagcat ggcattaatt accggccatc aagccacttc aggtaaccct
      541 tgcgaaggaa aactcaccga tcaatttggc caaatacatt atttgctact ggagcctgaa
      601 gaaggaaaaa ctttcactaa aggagacaaa gtgttaatta tttgccgact ttctgcaacg
      661 cgatatttgg cggaaaataa tccctggccc cagatattgt gatttattaa tcaaaaggaa
      721 atttaataat ggatgatatc gttaattctg tgccctcctg gatgtttacc gcgattatcg
      781 ccgtatgcat tctgtttatt attggaatta ttttcgccag gctctatcgt cgcgcttcag
      841 cagagcaagc atttgttcgt actggtttaa gtgggcaaaa agtggtgatg agcggtggcg
      901 caatcgtgat gccgatcttt catgaaataa ttcccatcaa tatgaatacc ctgaagctgg
      961 aagtcagccg ttctactatt gatagcctga ttacgaaaga tcgtatgcgt gttgatgttg
     1021 tagtggcttt ctttgtgcgg gtaaaacctt cagtagaagg gattgccacc gccgcccaga
     1081 cgctggggca acgcaccctg tcgcctgaag acttacgtat gctggtggaa gataaatttg
     1141 tcgatgccct ccgtgcaaca gcggcacaaa tgaccatgca tgagttacag gatacccgcg
     1201 agaacttcgt gcagggagta caaaataccg tagctgaaga tctgtcgaaa aatggcctgg
     1261 aactggaaag cgtttcactt accaacttta accagacctc gaaagaacat ttcaatccga
     1321 acaatgcctt tgacgccgaa ggtttaacca aactgactca ggagacggag cgccgtcgtc
     1381 gcgaacgtaa cgaagttgaa caggatgtgg aagtcgcagt gcgtgagaaa aaccgtgatg
     1441 cactttcgcg caagttggag attgaacaac aagaagcgtt tatgacgctt gagcaggagc
     1501 agcaggttaa aacccgtacc gctgagcaga atgcgaaaat tgcggctttt gaagctgaac
     1561 gtcgtcgtga agcagagcag acgcgaattc tggctgaacg acagattcag gaaacggaaa
     1621 tcgaccgcga acaggccgtc cgctcaagaa aggttgaggc cgaacgtgaa gttcgcatta
     1681 aagagatcga acagcagcag gtcaccgaaa tcgctaacca gacgaaatcg atcgctattg
     1741 ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc taaccttgca ctcgcagaag
     1801 cagtaagcgc ccaacaaaac gtagaaacca ctcgccagac tgccgaagcc gatcgtgcta
     1861 aacaagtggc gttgatcgcc gcagcgcagg atgcagaaac caaagcggtt gaactgaccg
     1921 tgcgggcgaa agcagaaaaa gaagccgcag aaatgcaggc ggcggctatc gttgagttag
     1981 ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc acaacgtgcg ctgaacgatg
     2041 ctatcaacgt actttctgat gaacaaacca gccttaaatt caaactggca ttattgcagg
     2101 cgctgcctgc ggtaattgag aaatccgtgg aaccaatgaa gtctatcgac ggcatcaaaa
     2161 ttattcaggt cgatgggctg aatcgtggtg gcgctacggg tgatgcaaac acgggtaatg
     2221 tggggggcgg aaacctggca gaacaagcgt tatcagccgc actctcttac cgtacacagg
     2281 caccgctgat tgactccttg ctcaatgaaa ttggcgtttc aggcggctca ctggcggcat
     2341 tgacttcatc cttaagctca acaactcccg tcgaagaaaa agcagagtaa accctcttct
     2401 atgaataatc cctggcccaa aaagccgggg attttttata tctgcgttcc gctaaaaggt
     2461 gcaaatgatc aagctgttgc agcattttgc gtggctgctc ggggaaggca aaactgcctc
     2521 tgggaaagcg ttgcgcgggg tccggcgctc atcaacaatc ggggagtcag caaggggctg
     2581 aaacgggaaa gcccctcccg aggaaggggc cttgtataag gaaagggtta tgatgaagct
     2641 cgtcatcata ctgattgtgt tgttactcgt aagtttcgca gcttattaac agccaatcag
     2701 aggggggaga aatcctccct cacccttgtt cctttacttt aggttgaaaa aaaatcagcg
     2761 tcaataggcc tgcctgctac aaagcgagat ctgtgaaccg ctttccggtt agcctttttt
     2821 atcctgttgg atcttcttga tgatgttggt cgtcgagcaa ccgtcttcaa agttgagcac
     2881 cagcacttcg ccaccgttgg cccagacttc tttactcccg gcaatctctt ctggtttata
     2941 gtcgccgcct ttcaccagca gatctggcaa gatcccggcg atcaagcgct gcggcgtgtc
     3001 ctcttcaaac gacaccaccc agtcgaccgc ttccagtgcg cccagcacaa tcatacgctg
     3061 ttcgagcggg ttaacggggc gggaatcccc tttcagccgt ttggtggagg catcgctgtt
     3121 cacagcgaca atcaaacggt cacccagttt acgggcattt gccagataag agacgtgccc
     3181 ggcgtgcagg atgtcaaaga caccgttggt catcaccact ttttcaccgc gtttacgcgc
     3241 tgccgctaca gcaagcttca gttcctcttc ggtcatcaca ccaaagccgg tatctgcacg
     3301 tccacgtact gcgttttcca gctcgatcgg cgaaaccgtg gatgtcccca gtttgccgac
     3361 caccacacca gctgccgcat tggcaaagaa gcaggcttct tccagcgaat tacccgctgc
     3421 cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca ccggtaacgt catacacttc
     3481 ctgcgcctgg gttggcatat gcagcggcgc tttacccggt tgcagcagcg acataccctg
     3541 ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg gcaatcagtt tcatgccgcg
     3601 ctcaacaatc tcttcttcgg tcttacattt accgacaaca gcttcaaatt ccgagagatt
     3661 cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg gtaccttttg gatcaatcag
     3721 caccggaaca cccgctttac gcgccagttg gatcatctgc tgtacgcttg ccagcgcacc
     3781 tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc agtgcctgat taatccgctc
     3841 gtgcagcggc tgcggatcaa ccccttcgaa accttcttcg aaatccagac ggatcagctg
     3901 ttggttacgg gaaagtaccc gtaatttggt gatagtcgga tgcgtcggta cagaaacaaa
     3961 gtcgcatttg acgttgacgt cggccagaga tttactgagc gcgcgcgccg catcgtcaat
     4021 gcccgtcaac ccgaccaggc gtgcattagc accgagagaa gcgatattca tcgccacgtt
     4081 agccgcgccg cccggacgtt cttcgatggt attcacttta accacgggca ccggcgcttc
     4141 cggcgagata cggctggtgg gaccgtacca gtaacgatcc agcatcacat caccaaccac
     4201 catcactcct gcacgttcaa actctggcag cgttactttc attcctgtct cctgagagat
     4261 tcaaaatttg cgcgcgataa taccatactt cattcttcca ccagccactt ctgccagctt
     4321 gcccgcacca gttcacgctc tgcggtgaag caatcctccg acacatggcc cggcaattcc
     4381 tgtaatgcca gatgatgaag ttcatcgcgc agcgtcgtgt aagcacgggt cagcgccatc
     4441 gcttcctgct cttccataat gtcgttttgc gccagtagtt ccagaatgcg cacgttgtct
     4501 gaccagcgcg ttaacttcgg tttttcgtga gcgtagcgca acaccagata ttgagtaata
     4561 aattcgatat cggtaattcc gccttcatcg gctttgatat caaagcgatc gcgatgttta
     4621 ttgccgagat gggcacgcat tttctcgcgc atttcccgca cttctgtttg cagggttttg
     4681 ccttcgcgcg gcagcgtcat aatctcccgg cgtactgcgt caaagtgcgc ggtgagctgc
     4741 ggatcgccgt acactacacg cgcacgcacc agcgcctgat gctcccacgt ccaggcctcg
     4801 tttttctgat aatcagcaaa tgcttctgcg gatgtcacca gcattcccgc cgccccggac
     4861 ggacgcagtc gtgcatccac ttcatacaaa atgccggaag aggttcgcgt actgaacaga
     4921 tgcataatgc gttgcgccag acgcagataa aactgtcgcc cgtcgatttc ccgctcgccg
     4981 tcagtcatcg catccattgg gcaatcatgg aggaagatca ggtcaagatc ggaactgtag
     5041 cctaactccc agccacccag cttgccgtag ccgaccaccg caaaaccacg cccttcgcgt
     5101 tcgttcaggt gatttggctt accgtagcgg gcaaccattt gaacccacgc ctgctgaacg
     5161 acggcatcaa tcatggcttc tgccagccag gttaagtgat cgctcacttt cattaccggt
     5221 agcgtaccgg cgatatccgc tgccgcaatg cgtaacagct gcgcctgttt gaactgacgc
     5281 agcgcctcaa gctgttgttc ttcgtcatct tccggcacgc gcagcaaata ctggcgcagt
     5341 tcatcacggt aggcatccgt cgccgttggt tggtagaggg tatttggatc gagcaattca
     5401 tccagcaata atggataacg cgctagctgg ctggcaatca tcggcgatgc agcacacaga
     5461 gaaatcaaat gcttaagcgc cgcagggaat tcactaagca gctcaagata agtggtgcga
     5521 gtaacaatcc ccaccagcaa ggcggtaatg cgcgacagcg tgacagcagc gtcttcacgc
     5581 gcacagacat cacttagcag atgtggcatc agatggtcga gcacctgacg tcctcgcggc
     5641 ccgatagtgc gcttatccag ctctttgcgg aaatcagcaa tcagcattag cacctgtttg
     5701 cgatcatcct cgctaagatg cgccagcact ggcgtggtgt catcttcctg caacgcatcc
     5761 tgccacagct cacgccactg ttccgacagc gactcttcct gagtttcact ttcgtcgtcg
     5821 ccaatcaatt cattaaacac ccggcgcaca ttggccatat gtgcggtcag caccccggtc
     5881 aattgcggcc agtcagcaaa gtccatcgcc cacgccagcc gcgcacggtt aagctcatca
     5941 gaaggaagcg tctgggtttg ttcgtcgtta atgctttgca gcaggttttc cagacgccgc
     6001 aggaacagat acgccactcg caattgttca gcatcgtttt cagaaagcag atgcagcgcg
     6061 gcaatggcgc tgagcgttgg cagtaaagcg cgcgattgca gcgacggttc gcgcccgccg
     6121 cgaatgagct ggaacacctg gacgataaat tcaatttcgc gaatgccgcc tgcgccaagt
     6181 ttgatgttgt cggtcaaacc gcgtcgacgc acttcacggg caatcattcc tttcatgttg
     6241 cgcagcgact gaatcacgct gaaatcgatg taacgacgga aaacaaacgg acgcagcatc
     6301 gcacgcaact cgttagcata gacgccgtcg ctatccccca taatccgcgc cttgaccatc
     6361 gcgtaacgct cccagtcgcg cccctgctcc tggtaataat cttccagcgc cgcaaaactc
     6421 agcaccagcg ggccactttc accaaacgga cgcagccgca tatccacgcg atagacgaag
     6481 ccatcctgcg ttggttgatc cagcacttta atcagccgct gccccatgcg ggtaaaaaac
     6541 tgcgcgttat ccagttcccg gcgtccaccc tgggtacaac catgttccgg ccaggcaaaa
     6601 atcagatcga tatcagagga gaaattcagt tccccgccgc ccagcttacc catgcctaaa
     6661 atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg ttccccattc gcggcagcag
     6721 gcgtcataca gccagtcacg cgccgcaaca atcagcgtct ccgccagatg gctgagctgc
     6781 tgcaatatgc tctcttcagt aaccagaacc agcgtttgcg cccaggcgat gcgcaccata
     6841 atgcgccgcc ggaatagccg cagctcgcgc attaacccgg cttcgtcact cacattactg
     6901 agcgcctcct gcaaccatgc agcgtaatgc tgccattcgt cggcctgcgg cggttggctt
     6961 tccagttccg tcagccactt tggatgcgca atcacgctgt cctgcacaaa atcactaaaa
     7021 gtaagtactg acttcgcctg tgcgctaagt gattcctcgg ctaaaggctc tggcagccgc
     7081 tcaacaacgg tctgccagta ctgctgtaac ggtgaagaga gcggcttcat aagcgatttt
     7141 atccttgcct gattaacgtt ttccgctgtg caaccagaac ggttcctgat tgtttgcctc
     7201 attacggaaa tgttcaattt caatgcgttg cccggtcgca atagcgtgac gcagcccctg
     7261 ccagttctcc agccaggctt gcgcgacgac aggatcgtag taacccgcca gcaacagtat
     7321 tgagtcaata tcacgcgtca ggcgtggcaa ttggtcacgg tagcgatcgc ctaacggctg
     7381 gcagaaaacg cttttaagtt cagcagcatg gcgggaaaga tggatatcgg caaagcgttt
     7441 gaaggaatcg ctcattttac tctgcgcttt ggcatctaaa aacggctgcc aggctttgct
     7501 taccagccat tcggtcaacg ccagcttcgc cattgccgtt tcggtagagt agaccgccgt
     7561 tacggcagaa accgcagaag caatggtcgc ctcgcattga gtcagcagat cacgtaagtg
     7621 agtgctcgct ttacgcggca cgataccgcc gaacagcatc agtgtatgac ggaccaggcc
     7681 aatggctgcc agcacctgct ctttcgccgc atcgttgccg cgtacccaca gttcttcatg
     7741 atactgccat tgcgctaacg ccagctcgag cgccgcttcc agcccctgtt ccacatcagc
     7801 ttttgccgca acatgcaaaa tggtggtcgg tttgatttca cgcgccggat tgccctgcgc
     7861 cagatgatag ccacgcgctg ctttgctcag gctgccctgg cgtaatccgg tttgcgatac
     7921 cagttggttc gccagtttca gcaccgcacg catgtcgccg ctaagcagtt ccagttccag
     7981 ctcacagata ggttcagcaa attcacccgc tttcacttcc ccctggtcga gggcgatttc
     8041 aatttggcta ccatcaacct ctaccagcca cttttcgcga taaaaatcgg tgctgaacag
     8101 cggttgtacg cgggaagcga gatcggcagg caattcgccg ttcggccaga cttccgtcgg
     8161 taactgcgcc aggtcgagcg tcggttcgct caacgccacg ttatattccg ggcgctggtg
     8221 taagccgcca gtcactcttc ctgcaacttt catggtcatc tcatagcgac cgttttcgcc
     8281 acgcatacgc aagcccatgt cgtgcccacg cagccagtta tccggcgttt cgtagtaaat
     8341 attcagcaac tgcacggggt catggtgctc gccgcccagc gtattaagat ggccacgtag
     8401 cgcctcaacg gcactgtgat taacaataaa ctttaattcg atttcctgag ccatggtctt
     8461 tacttattag ttgtgtcaca taagtgacga tgaacggcga acttaatgcg atcttttttg
     8521 tcagtagata gtattttgcg ccaaattgcc atgcaacggg caatttgacg ggcgtaaaag
     8581 tttgaagcag tggcaagacc tgacacagag gattccgatt gatgtcgaat gctttgcgta
     8641 gcgacactga tgccactact atcgttccac tttccatgac aataacgaca gcctgatgcc
     8701 aaaattacgc ctgatcggat taactttact cgcacttagc gccactgccg tctcacatgc
     8761 tgaagaaacg cgctatgttt ctgatgaact gaatacctgg gtccgtagcg gtccgggaga
     8821 tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa gtgaccttat tacaaactga
     8881 cgccaacacc aattatgccc aggtgaaaga cagctctggc cgtaccgcct ggatcccgtt
     8941 gaaacaactt agcacagagc caagcctgcg ctcccgtgtg ccagatctgg aaaatcaggt
     9001 caaaacgctg accgataaac tcaccaatat cgataacacc tggaatcagc gcaccgcaga
     9061 aatgcagcaa aaagtggcgc agagtgacag cgtgatcaac gggttaaaag aagaaaatca
     9121 gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc gatgccgcca gcgtacagct
     9181 ggatgacaaa cagcgcacca tcatcatgca gtggtttatg tatggcggtg gcgtgctggg
     9241 gcttggcttg ctgctcggtc tggtactgcc gcacctgatc ccaagccgca aacgcaaaga
     9301 tcgctggatg aactaaatcg ccttcttcgt tgcactgtct tattattagg aattgtgtat
     9361 ttgacgaagg gatgatggcg tgaagattta tctggtcggt ggtgctgttc gggatgcatt
     9421 gttagggcta ccggtcaaag acagagattg ggtggtggtc ggcagtacgc cacaggagat
     9481 gctcgacgcg ggctaccagc aggtaggccg cgattttcct gtgtttctgc atccgcaaac
     9541 gcatgaagag tatgcgctgg cacgtaccga acggaaatcc ggttccggtt acaccggttt
     9601 tacctgctat gccgcaccgg atgtcacgct ggaagatgat cttaagcgtc gcgatctgac
     9661 cattaatgcg ctggcccagg acgataacgg cgagattatc gacccgtaca acggtctggg
     9721 cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct tttggcgaag atccgttacg
     9781 cgtgttgcgc gtagcgcgtt ttgctgcgcg ttatgcccac ctcagtttcc gtattgccga
     9841 tgaaactctg gcgctgatgc gcgagatgac ccatgcgggt gaactggaac acctgacgcc
     9901 tgaacgggta tggaaagaga cggaaaacgc cctcaccacc cgcaatcccc aggtgttctt
     9961 ccaggtactg cgcgattgcg gcgcactgcg cgttttattc ccggaaattg acgccctgtt
    10021 tggcgttccg gcccctgcca ggtggcatcc ggaaatcgac acgggtattc ataccttaat
    10081 gacgctctca atggcggcga tgctgagtcc gcaggtcgat gtccgtttcg caacgttatg
    10141 ccacgatctc ggtaaaggac tgacgccgcc agaactttgg ccacgtcatc atggtcatgg
    10201 cccggcgggt gtgaagttag tggaacaact atgccagcgt ctgcgcgtgc caaatgaaat
    10261 tctcgattta gccagactgg tggctgagtt tcacgatctc atccacacct ttccaatgct
    10321 gaacccgaaa accattgtta aattgtttga ttccattgac gcctggcgta aaccgcagcg
    10381 tgtcgagcaa ctggcgctga ccagcgaggc tgacgtgcgc ggcagaaccg gttttgaatc
    10441 agccgactac ccgcaaggcc gctggttgcg cgaagcctgg gaagtggcac agtcagtgcc
    10501 gacaaaagcc gtcgttgaag cgggatttaa aggtgtggag attcgcgagg agctgacccg
    10561 acggcggatt gcggcggtgg ctggctggaa ggaacagcgt tgcccaaaac ctgagtgagt
    10621 gttggatatt ttgccggatg cgacgtaagc gtcttatccg gctacatgtc aacgacagtt
    10681 gtaggcctga taagacgcgc gagcgtcgca tcaggcgttg attgccggat gcggcgtgaa
    10741 cgccttatcc ggcctacgca atctgataag acgcgtcagc atcatatccg acactgttct
    10801 tcacatcaaa agaacacaac gtacaccgca gccgccacaa taaagcgata aatggcgaac
    10861 gggataaacg aaatgcgctt aatcaattgc aggaaggttt taatcgctat cagcgccacc
    10921 acaaaagcgg tgataaaccc aacggcaaac atcgggatat cgccggtagt caggaagccc
    10981 cagcttttgt agagatcgag cgccgttgcg cccatcatca tcggcaccgc cagcaggaac
    11041 gagaactcgg aagcagcgta acggctcacc cccatcagca tcccacctga aatggtcgcc
    11101 ccggaacggg agaaacccgg ccacagcgcc agacactgga aacagccaat cataaacgcc
    11161 tgacgatagg tcatatcatc aagacccggc gcacgcggct ctttcggctt caggcattcg
    11221 gcggcaatca gcaacagacc gccaacgacc agtgcataca tgacatttat cgggttaaac
    11281 aatgatttaa tcgtgtcgtg gaacaacagc cccagtacca ccgccggaat catccccagc
    11341 aagatgtgga tcagcgttaa acgacctttg ctttctcctt cgtgctgcaa cgggcgacca
    11401 aagtggatgc caatcaggcc aaacagacgc cgccagaaca tcactactac cgccagaatt
    11461 gatcctaact ggatcacgac ttcaaaggtt ttcgccgtgt cgccctcaaa ccccaacaag
    11521 tgaccgacaa taatcatatg gcccgtgctg gataccggca gaaattctgt caatccttcg
    11581 accacaccca atattgccgc tatcagcagc gagtgcatat cgctcatcaa taaaccccta
    11641 aattataaaa atgtaacgct tgtccgaact actgcagtat gaccaggtta taatcgtttg
    11701 gtttaacagc tgtaaaatta attattttct ttcagattat tgccacgctc aatgattacg
    11761 ccaacattcg ccgcccgcgc cactgcgcct ggcttgctga gtttgatacg cacccacggc
    11821 gagttgaagc gtgctaacag cagctccgcc acctcttcag ccacgcgttc caccagcgca
    11881 aaacgcgccc cctcgacgtg gctgaccacc gtttctgcaa tgtcagcgta actgaggcaa
    11941 tccgccacat catcactttt cgccgcttta cggttatccc acgccatttc gatatcgaac
    12001 actaacttct gttcgatggt ctgttcccag tcgtaaacac caatagtggt gattaccgaa
    12061 agttgctcta taaatacaat atccatcacg tcctgcctgc tttttggcta accggatacc
    12121 acttccggcg aaatgtgcgt attatccaca gattcatcgt tgaacacgaa ttttcaaaac
    12181 ggaacagctt atgagtgcaa tcgcgcctgg aatgatcctc atcgcgtacc tctgcggctc
    12241 catttccagt gccattctgg tttgccgctt gtgtgggttg cccgatccgc gaaccagcgg
    12301 ctccggcaat ccaggcgcaa ccaatgtgtt acgtatcggt ggcaagggag cagccgtagc
    12361 agtactgatt ttcgacgttc tgaaaggaat gttgcccgtc tggggcgcgt atgaattagg
    12421 tgtcagcccc ttctggctag gcttaattgc catcgccgcc tgtcttggac acatctggcc
    12481 cgttttcttc ggttttaaag gaggaaaagg cgttgctacc gcttttggtg ccatcgcacc
    12541 aattggctgg gatctcactg gagtaatggc gggaacctgg ttactgaccg tgctattgag
    12601 tggttactcg tcgctgggag cgattgtcag tgcactgatt gctccgtttt atgtctggtg
    12661 gtttaagcca caattcacct tcccggtttc gatgctctct tgcctgatcc tgctgcgtca
    12721 tcatgacaac atccagcgtc tgtggcgtcg tcaggagaca aaaatctgga cgaaattcaa
    12781 aagaaagcgc gaaaaggatc ccgagtgatt tctggtggat ctacatgacc tgatagcctt
    12841 cgtcgggctt gcccagccgt tgctggcacc atgccgccag aaattccaca cagacacgta
    12901 atttcatgct gcgatagagc ggctcccggt aaacagccca gatattggcg ctttgtgcat
    12961 actccggcaa tactcgcacc agtttgccac tctccagaaa cggcaacaca tcccactcgg
    13021 aacgcagcat aatccctttg ccctccagcg cccattgcag cacaatctcg ccgctattgg
    13081 aggaaagatg tccgcttacc ttcaccgatt ttttctcctg accgttcccc aactcccata
    13141 ttccgtgggt catatcgcgc tctttggtca ccaggcagtc atgacgactt aattcctgta
    13201 aggattgtgg ctgcggatat ttttgcagat actcaggtgc tgcgcataat attcttttat
    13261 tttttgtcaa aagatgcgca atataataat caggaattgc gtcattaata cgaatatcca
    13321 gatcaatatt atcctgcact aaatcaattt gtcgatcgaa caattcaaaa tgcacctgta
    13381 actcaggata attgcgcatc agttcggtaa tagctggcgc aatatggctg cgcccaaaac
    13441 caaaactgca accaatgcgg atcatcccct ccgggcgcgt tttgatttgc gtgacgtcat
    13501 cgaccagtcg ctgatattgg gtgaggattt ccagcgcatg ttcgtaacaa cgctggccac
    13561 tttcagtcag cgccacgccg cgagcggagc ggttcagcag agttgttgcc agcgtatttt
    13621 caaggatttg gatccgcttg gtgacaaatg caggcgtttg cccaagcgtc gccgctgcag
    13681 cactaaaact cccgctatgg acgatttcca ccagtacctg caagtctttg gctaaaggcc
    13741 agctatttag cattgttaaa tatccgcagt tttgataaac gcagtgtatt tcgcaaaaca
    13801 ttaccattcc ctgctctggc gcaatattcg ttcttatttt cgcgttgatt cacgaactgt
    13861 taattacata ttcacggtgg caaaaaatat aaaaccacat ttttagtggt agtttgtggc
    13921 ggtgaatttt tccagacaaa tacataaact ggagttgcca tgatgagcga aagtaataag
    13981 caacaggcag tgaataagtt gacggagatt gtcgctaact ttaccgccat gatttctacc
    14041 cgaatgcctg atgacgtggt ggataaacta aaacagctaa aggatgccga aacatcgtcg
    14101 atgggaaaaa tcatctacca cacgatgttc gataacatgc aaaaagcgat cgacctgaat
    14161 cgtcctgcct gtcaggacac cggcgaaatc atgttttttg ttaaggtcgg ttcccgtttc
    14221 ccactgcttg gcgagctgca aagcatactc aaacaagccg tggaagaggc aaccgtcaaa
    14281 gcgccactgc gtcacaatgc ggtagaaatt tttgacgaag taaacaccgg caaaaatacc
    14341 ggcagtggcg taccgtgggt cacctgggat atcgtccccg acggtgacga tgcggaaatc
    14401 gaagtttaca tggcaggcgg cggctgcacg ctacctggcc gatcgaaagt gttaatgccg
    14461 tcagaaggct acgaaggcgt agtgaaattc gtcttcgaaa atatctccac cctcgccgta
    14521 aacgcctgtc cgccggtact tgtgggggtt ggcatcgcta cctcggtgga aaccgccgca
    14581 gtgctctcgc gaaaagccat tttgcgcccg attggcagcc gccaccccaa tccgaaagcg
    14641 gcagagctgg agctacgcct ggaagaagga ctcaaccgtt tggggattgg tccacaaggg
    14701 ctgactggca acagttcagt gatgggcgtg catatcgaat ctgccgctcg ccatccgtca
    14761 accatcggtg ttgctgtttc taccggctgc tgggcgcatc gtcgcggcac actgcgagtt
    14821 catgccgatc tcacctttga aaatctgtct catacccgga gcgcgttatg aaaaagatcc
    14881 tgacaacccc gatcaaagct gaagatctgc aagatattcg cgtcggcgat gtgatctacc
    14941 tgaccggtac gctggtgacc tgccgtgacg tttgtcaccg ccgattgatc gaactgaaac
    15001 gtccgatccc ttacgatctc aacggcaaag cgattttcca cgctggcccc atcgtgcgca
    15061 aaaacggcga caaatgggag atggtgtccg tcggcccgac aaccagtatg cgtatggaaa
    15121 gttttgaacg tgaatttatc gagcagaccg gcgtgaaact ggtggtcggc aaaggcggaa
    15181 tggggccgct gaccgaagaa ggctgccaga aattcaaggc gctacatgtg attttcccgg
    15241 caggctgcgc agtactggcg gcaacccagg tggaagagat tgaagaagtg cactggacag
    15301 aactcgggat gccagagtcg ctgtgggtct gccgggtcaa agagtttggc ccgctgattg
    15361 tctctataga tacccacggc aacaacctga tagccgaaaa caaaaagctg ttcgccgaac
    15421 gccgcgatcc catcgtggaa gagatttgcg agcacgtcca ttacatcaaa taaccctcac
    15481 ggagaggctc gcccctctcc tttttctcag gcataacacg atgaaacctt ccactgaatg
    15541 gtggcgatac cttgcgccgc tggcggtcat cgccattatt gctctacttc cgcttcccgc
    15601 agggctggag agtcatacct ggctctactt tgccgttttt actggcgtga tcgttggact
    15661 gatcctcgaa cccgtgccgg gtgccgtggt ggcgatggta ggtatatcca tcatcgccat
    15721 tctttcgccg tggctgctgt ttagcccgga gcagctcgcc cagccaggtt ttaagttcac
    15781 cgccaaatcc ctctcctggg cggtttctgg tttttctaat tcagttatct ggcttatttt
    15841 cgccgccttt atgtttggta cgggctatga aaagaccggg cttggacgtc gcatcgcgct
    15901 gattctggtg aaaaaaatgg ggcatcgcac gctgtttctc ggctatgcag tgatgttctc
    15961 cgagctgata ctcgcgcctg taacaccgtc caactcggcg cgtggtgcgg ggattatcta
    16021 tcccatcatc cgtaacctgc caccgctcta tcaatcacaa ccaaacgaca gcagttcgcg
    16081 cagcattggc tcgtacatca tgtggatggg gattgttgcc gattgcgtga ccagcgccat
    16141 tttcctgacg gcgatggctc caaatttgct gttaattgga ctgatgaaaa gcgcatctca
    16201 cgccacgctg agctggggcg actggttcct cgggatgttg ccgctcagta ttttactggt
    16261 tctgctggtt ccctggctgg cttacgtgct gtacccgccg atactgaagt ctggcgatca
    16321 ggtgccgcgc tgggcagaga cggaactgca ggcaatgggc ccgctctgtt cgcgtgaaaa
    16381 acggatgctg gggctgatgg taggcgcact ggtgctgtgg atttttggcg gtgattatat
    16441 cgatgccgcg atggtcggtt acagcgtggt ggcgctgatg ctgcttctgc gcattatcag
    16501 ttgggacgac attgtcagta ataaagcggc gtggaacgtt ttcttctggc tggcctcgct
    16561 tatcaccctc gctaccggac tcaacaacac cggttttatt agctggtttg gcaaactgtt
    16621 agcaggcagc ttaagcggtt attcgccaac gatagtgatg gtagcgttga ttgtggtctt
    16681 ttatctgctg cgctactttt ttgccagcgc cacagcgtat acctccgctc tcgcaccgat
    16741 gatgattgcc gccgcgctgg cgatgccgga aatcccgctg ccggtgttct gcctgatggt
    16801 tggtgcggca attggtctgg ggagcattct tacaccatac gccaccggac ccagtccgat
    16861 ttactacggc agtggttatc tgccaacggc ggattactgg cgactggggg cgatttttgg
    16921 gctgatattc ctcgtattgc tggtgattac cggcttactg tggatgcccg tggtgttgct
    16981 ttaagtgagg acggtgccgg aaattgctct ccggcaccca atagttttac gcagccggta
    17041 actccgccag cggccagcgc ggacgcacac tcacgccgag atccgccgtc gcgcctgctt
    17101 taaaccgcac cattccggca taggcgatca tcgcaccgtt atcagtgcaa aattccggac
    17161 gcgcgtagaa cacttcgcca cgacgctttt tcatcatttc agccagcttc gcccgcagtg
    17221 tgcggttagc actcacgccg cctgccatga ccagtcgctt aaagcccgtc tgatccagtg
    17281 ctcgcttaca cttaatcatc aacgtatcga ccactgcatc ttcaaaggcg cgggcgatat
    17341 cggcacgcgt ctgatcgtca gtgccgttgt cacgtatggt atttgccgcg aaggttttca
    17401 gaccagaaaa gctgaaatcc agccccggac ggtcggtcat cgggcgcggg aagacaaagc
    17461 gcccggcagt accctgagcc gccattttcg acagtaatgg cccgccagga taatccagcc
    17521 ccagcagctt cgcggtttta tcaaacgctt caccagccgc atcatcgata gactctccga
    17581 gcagctcgta ctgaccaatg ccagtcacgc tgattaactg cgtatgaccg ccggaaacca
    17641 gcagcgcgac aaacggaaat tccggtgggt tatcttccag catcggcgct aacagatgtc
    17701 cttccatatg atgtaccggg attgccggaa cgtcccaggc aaacgccaga gaacgcccga
    17761 ccgttgcgcc aaccagcagt gcgccgacta atccagggcc tgcggtatag gccacagcat
    17821 caatgtcttt tgccgttaaa ccagactctt tcagcgcctc ctggatcaac ggtacggttt
    17881 tacgcacgtg atcgcgggag gccagttcag gcacgacgcc gccgtagtca gcgtgcaatt
    17941 tcacctgact atacaattgg ttggctaaca aacctttttc atcgtcgtaa atggcgatgc
    18001 cggtttcatc gcaggaagtt tcaataccca gtacacgcat gacttgtttt acctcgcttt
    18061 attaccgcgc agtgtaggac caatgcgggt tgatgtaaaa ctttgttcgc ccctggagaa
    18121 agcctcgtgt atactcctca cccttataaa agtccctttc aaaaaaggcc gcggtgcttt
    18181 acaaagcagc agcaattgca gtaaaattcc gcaccatttt gaaataagct ggcgttgatg
    18241 ccagcggcaa accgaattaa tcaaaggtga gaggcacatg ccggtaatta aagtacgtga
    18301 aaacgagccg ttcgacgtag ctctgcgtcg cttcaagcgt tcctgcgaaa aagcaggtgt
    18361 tctggcggaa gttcgtcgtc gtgagttcta tgaaaaaccg actaccgaac gtaagcgcgc
    18421 taaagcttct gcagtgaaac gtcacgcgaa gaaactggct cgcgaaaacg cacgccgcac
    18481 tcgtctgtac taattccccg agagcgttgc tctccgatca gaccgagttg tagttgtaag
    18541 gccgtgcttc cgaaaggaat gcgcggctta ttttcgttta tgaattgcta aaaatcgggg
    18601 cctatggctg gacgaatccc acgcgtattc attaatgatc tgctggcacg cactgacatc
    18661 gtcgatctga tcgatgcccg tgtgaagctg aaaaagcagg gcaagaattt ccacgcgtgt
    18721 tgtccattcc acaacgagaa aaccccgtca ttcaccgtta acggtgagaa acagttttac
    18781 cactgctttg gatgtggcgc gcatggcaac gcgatcgact tcctgatgaa ctacgacaag
    18841 ctcgagttcg tcgaaacggt cgaagagctg gcggcaatgc acaatcttga agtgccattt
    18901 gaagcaggta gcggccccag ccagatcgaa cgccatcagc ggcaaacgct ttatcagttg
    18961 atggacggtc tgaatacgtt ttaccaacaa tctttacagc aacctgttgc cacgtctgcg
    19021 cgccagtatc tggaaaaacg cggattaagc cacgaggtta tcgcccgctt tgcgattggt
    19081 tttgcgcccc ccggctggga caacgtcctg aagcggtttg gcggcaatcc agaaaatcgc
    19141 cagtcattgg ttgatgcggg catgttggtc actaacgatc agggtcgcag ttacgatcgt
    19201 ttccgcgagc gggtgatgtt ccccattcgt gataaacgcg gtcgggtgat tggttttggc
    19261 ggacgcgtgc tgggcaacga tacccccaaa tacctgaact cgccggaaac ggacattttc
    19321 cataaaggcc gccagcttta cggtctttat gaagcacagc aggataacgc tgaacccaat
    19381 cgtctgcttg tggtcgaagg ctatatggac gtggtggcgc tggcgcaata cggcattaat
    19441 tacgccgttg cgtcgttagg tacgtcaacc accgccgatc acatacaact gttgttccgc
    19501 gcgaccaaca atgtcatttg ctgttatgac ggcgaccgtg caggccgcga tgccgcctgg
    19561 cgagcgctgg aaacggcgtt gccttacatg acagacggtc gtcagctacg ctttatgttt
    19621 ttgcctgatg gcgaagaccc tgacacgctg gtacgaaaag aaggtaaaga agcgtttgaa
    19681 gcgcggatgg agcaggcgat gccactctcc gcatttctgt ttaacagtct gatgccgcaa
    19741 gttgatctga gtacccctga cgggcgcgca cgtttgagta cgctggcact gccattgata
    19801 tcgcaagtgc cgggcgaaac gctgcgaata tatcttcgtc aggaattagg caacaaatta
    19861 ggcatacttg atgacagcca gcttgaacga ttaatgccaa aagcggcaga gagcggcgtt
    19921 tctcgccctg ttccgcagct aaaacgcacg accatgcgta tacttatagg gttgctggtg
    19981 caaaatccag aattagcgac gttggtcccg ccgcttgaga atctggatga aaataagctc
    20041 cctggacttg gcttattcag agaactggtc aacacttgtc tctctcagcc aggtctgacc
    20101 accgggcaac ttttagagca ctatcgtggt acaaataatg ctgccaccct tgaaaaactg
    20161 tcgatgtggg acgatatagc agataagaat attgctgagc aaaccttcac cgactcactc
    20221 aaccatatgt ttgattcgct gcttgaactg cgccaggaag agttaatcgc tcgtgagcgc
    20281 acgcatggtt taagcaacga agaacgcctg gagctctgga cattaaacca ggagctggcg
    20341 aaaaagtgat ttaacggctt aagtgccgaa gagcgatcgg gaagcccccg acagccgcac
    20401 tgagaggcag cggcaaatat ataagtacgc cctcgtaatt atcgttggcg gtaaacaacc
    20461 gttggatttc agcgttaaca gctgaaggac atcgggtcaa tcgcccaaca ccaacctcat
    20521 gaaataagtg tggataccgt cttatggagc aaaacccgca gtcacagctg aaacttcttg
    20581 tcacccgtgg taaggagcaa ggctatctga cctatgccga ggtcaatgac catctgccgg
    20641 aagatatcgt cgattcagat cagatcgaag acatcatcca aatgatcaac gacatgggca
    20701 ttcaggtgat ggaagaagca ccggatgccg atgatctgat gctggctgaa aacaccgcgg
    20761 acgaagatgc tgccgaagcc gccgcgcagg tgctttccag cgtggaatct gaaatcgggc
    20821 gcacgactga cccggtacgc atgtacatgc gtgaaatggg caccgttgaa ctgttgaccc
    20881 gcgaaggcga aattgacatc gctaagcgta ttgaagacgg gatcaaccag gttcaatgct
    20941 ccgttgctga atatccggaa gcgatcacct atctgctgga acagtacgat cgtgttgaag
    21001 cagaagaagc gcgtctgtcc gatctgatca ccggctttgt tgacccgaac gcagaagaag
    21061 atctggcacc taccgccact cacgtcggtt ctgagctttc ccaggaagat ctggacgatg
    21121 acgaagatga agacgaagaa gatggtgacg acgataccgc cgatgatgac aacagcatcg
    21181 acccggaact ggctcgcgaa aaatttgcgg aactgcgcgc tcagtacatt gtaacgcgtg
    21241 acaccatcaa agcgaaaggc cgcagtcacg ctgccgctca ggaagagatc ctgaaactgt
    21301 ctgaagtatt caaacagttc cgcctggtgc cgaagcagtt tgactacctg gtcaacagca
    21361 tgcgcgtcat gatggaccgc gttcgtacgc aagaacgtct gatcatgaag ctctgcgttg
    21421 agcagtgcaa aatgccgaag aaaaacttca ttaccctgtt taccggcaac gaaaccagcg
    21481 atacctggtt caacgcggca attgcgatga acaagccgtg gtcggaaaaa ctgcacgatg
    21541 tctctgaaga agtgcatcgc gccctgcaga aactgcagca gattgaagaa gaaaccggcc
    21601 tgaccatcga gcaggttaaa gatatcaacc gtcgtatgtc catcggtgaa gcgaaagccc
    21661 gccgtgcgaa gaaagagatg gttgaagcga acttgcgtct ggttatttct atcgctaaga
    21721 aatacaccaa ccgtggcttg cagttccttg acctgattca ggaaggtaac atcggtctga
    21781 tgaaagcggt tgataaattc gaataccgcc gtggttacaa gttctccacc tacgcaacct
    21841 ggtggatccg tcaggcgatc acccgttcta tcgcggatca ggcgcgcacc atccgtattc
    21901 cggtgcatat gattgagact atcaacaagc tcaaccgtat ttctcgccag atgctgcaag
    21961 agatgggccg tgaaccaacg ccggaagaac tggctgaacg catgctgatg ccggaagaca
    22021 agatccgcaa agtgctgaag atcgccaaag agccaatctc catggaaacg ccgatcggtg
    22081 atgatgaaga ttcgcatctg ggggatttca tcgaggatac caccctcgag ctgccgctgg
    22141 attctgcgac caccgaaagc ctgcgtgcgg caacgcacga cgtgctggct ggcctgaccg
    22201 cgcgtgaagc gaaagttctg cgtatgcgct tcggtatcga tatgaacacc gaccacacgc
    22261 tggaagaagt gggtaaacag ttcgacgtta cccgcgaacg tatccgtcag atcgaagcga
    22321 aggcgctgcg caaactgcgt cacccgagcc gttctgaagt gctgcgtagc ttcctggacg
    22381 attaatcggt agaggcgtta cgccgcaccc ggcactagtc cctctgcaca aacgccacct
    22441 tttcggtggc gtttttatcg cccacgcacc accagcgcct ggtccagctc gcgatacgct
    22501 tcaaccagtt tctccagtga aacgcgactt aaaccgctgg gatttggcag gacccaaatc
    22561 tgcgtcgaac caatggtgag agtttgtttc ccccactgtg caccgcgctg gctgaatccc
    22621 tgttcatatg cttgtttgcc cagaatcgcc aacgcctgcg gctgataatc ttctattttt
    22681 tcaattagct tacgcccgcc tgcgtgcagc tcctgctttg acacttcatt ggcttgcacc
    22741 gttggacggt ctaccagttt ggtgacgcca caacgataat ccagcagatg ctgtgcctcc
    22801 tgcggcttca actgacggtc ggtaaacccg gcctgatata tcaccttcca gaagcggttt
    22861 gccggatgag caaagggaaa acccgttccg gcggatgaaa gaccagggtt gataccgcaa
    22921 aacacgaccc gtaaccctgg agccaaaata tcctcaacca tatttactcc tttctaacat
    22981 caccaggaaa agtataacgg attgaaaata cattgtttat aaaaacagca ggcgcgcggt
    23041 aatggctgga ttgcgacacg gagttacttt ataatccgct accatggccc cttagctcag
    23101 tggttagagc aggcgactca taatcgcttg gtcgctggtt caagtccagc aggggccacc
    23161 agacatagca aaggctgacg agaaatcgtc agcctttttc tttttatata tcagttactt
    23221 tgcgtgccag taagccgccg cgcgtacccg ctgtgggtca tactgttccg cttcaaagcg
    23281 acggcttaaa ttcttaacga ctttgccttc gccggttatc cagatgaagt aatcatcggc
    23341 agggatttgc atctgcgcca gacgcgcatc taccgcctgc tcatcatgtg ccagccattc
    23401 gatattaaaa ccatcaaggt gcgcgagata atcctgacag gcgttatccc gcacgctaac
    23461 cagcgcgcta acttgcggtt taacggcaag tttgctcaac gtttccaggc ggcggcgcag
    23521 tgcgggcatt ccggattcat cgcagacata cagctgatac gcgtaatcct ctggcaccac
    23581 cagcgaaccg cgcggacctg ccaccgtgag tttatcgccc gattgcgcct gcattgccca
    23641 cctgctggcg acgccacctt cgtgaataaa gaaatcaatc gccagttcat ggcgtagttc
    23701 gtcatacagc ggcgtatagt cacgcgacgg tgggcgtggt ccttccggcc agacgatgcc
    23761 ctcttccgtt accgttggcg gcacaaagtg agcgtcaggt tgaggaaaga agagtttgct
    23821 gtgatcgtca aagccgcgcg atgtaaaacc gtccagcgcc tcgccgccga ggacaatgcg
    23881 ctgaaaaccg gcgctgatgc gctcaacgcg taacacagtc agttcacgga agcgcagatc
    23941 attgcgaacg cgctgcgggt agcgggggga gttattcatt gttatcgcct tcgtgatggt
    24001 aatcagatat atctaaataa ttttcgtaaa tgataatgat tgttaatcat gataaatgca
    24061 agcgatttgt agaactgata tgtctatagt ctgataagac gaaccgcctc ttctcaggca
    24121 tcattactca acgccggatg cggcgtgaac gccttatccg gcctacgtgt gagatgagtc
    24181 cacgtttaaa aatcattttt attcttgcaa gagtgctcat atcagatata aattagatat
    24241 atctaatcaa gtaaaaggag gctgatatga gccatcatca cgaagggtgt tgtaaacatg
    24301 aaggccagcc acgccatgag ggctgctgca aaggtgagaa gtcagaacac gagcactgcg
    24361 gacacggtca ccagcatgaa cacggtcaat gctgcggtgg tcgccacggt cgcggcgccg
    24421 gtcgtcggca acgtttcttt ggtcacggtg aattgcgtct ggtgattctg gatattctct
    24481 cgcgcgatga cagccacggt tacgagttga ttaaagcgat tgaaaatctg acccagggga
    24541 attacacccc aagcccgggc gtcatctacc caacgctgga ttttctgcag gagcagtcgc
    24601 tgattaccat ccgtgaagag gaaggtggta agaagcagat tgcgctgacc gaacaaggcg
    24661 cgcagtggct ggaagaaaac cgtgaacagg tagagatgat tgaagaacgc atcaaagcgc
    24721 gttgcgttgg cgctgcgctg cgccagaacc cgcaaatgaa gcgggcgctg gataatttta
    24781 aagcggtgct ggatttacgc gtcaaccaga gcgatatcac tgatgcacaa ataaaaaaga
    24841 tcattgcggt gatcgaccgc gccgcttttg atattaccca actggattaa tcaccgattg
    24901 ccggatgcag cgtaccctac acaccccgta ggcctgataa gatgcgccag catcgcatca
    24961 ggcattgtgc tccaaccgcc ggatccggca taccgattaa tgcagcaccg tcaccgcgtc
    25021 ttccagtcgg ctggcgcggt gtttcaccat cgccgacacc tgcgcactct cttccaccaa
    25081 ctcggcgttt ttctgggtga tcaggttaag ctcatccact gcacgggtca ggctggaaag
    25141 cccatcggcc tgttccagcg ttgaatggct aatctgagct atcaactggg tgacgttttt
    25201 tacctgcgcc acaatatctt ccatcgtccg tccggcggcg tgtacctgct gcgaaccaga
    25261 ttgcacctta tcagcactgg catcaatcag cttgcgaata tcgttggcag cattggcgct
    25321 gcggctggct aaatgacgca cttcccccgc caccactgca aaacctttgc cctgttcgcc
    25381 ggcacgcgcc gcttccaccg ccgcattcag ggccagaata ttggtctgaa acgcaatatc
    25441 gttaatcagc gaagtaatgg tgccaatgcg ctgggtactg tcggcaatat cgtccattgt
    25501 cttgatcacc gtagtcatcg cctccccgcc ctgcactgcc gcattactgg cggtgataga
    25561 aagtttatcg gcagccgacg ccgtggcaga gttctgtttc accgacgccg ccatttggtt
    25621 catggtcgcc accgtttgct gaacgttatc aactgtctgc tgggtatgtt cgttcagttc
    25681 atcggtgcct ttcgccagcg tctcactgcc gtttctgaca ctggacacct ggcttgagac
    25741 atcattaatt agccaacggc acatcaggcc aagctgccct accgcacgta atgtcagccc
    25801 cagctcatcg ctgcgcttca gatgctcaac actattacgt tctccggtcg ccactttcag
    25861 tgcctggctg gcgacatttt ctatcggacg tacaatttgc cattcaaaac aggcatttcc
    25921 caacaacatc actaacgcac agaggaaata cgtcaccacc ggggcagcga caaaccagag
    25981 catggccgcc agcacgataa acatcagggt catcactcca cgcacccgcc agcgaagcgg
    26041 taatgaaggc agtttaccca gccagccttt acgcaccacc aggcctttat gaatacgctt
    26101 accggtacgt ccggcgttca gcgctttgta cagcggctcc accgccgcga tctcttcatc
    26161 cgtcgcccgg gtacgaatcg acatatagcc actgattttt ccctcgcgca ccatcggtac
    26221 cgcattggcc cgcacccaat aatggtcgcc atttttgcgg cgatttttca cgatgccgct
    26281 ccagggttcc ccttttttca gggtgaacca catatccgca aacgccgctt ttggcatatc
    26341 cgggtgacgc accatgttgt gcggttgccc ctgtaactcc tgcaaggtat agccgctcac
    26401 ctgcacaaaa gtgtcattag catgagtaat atagctttgc agatcggtag tggacatcag
    26461 agtggtgtcg tccgccagcg gggtattttg ctgggtgaca tacggatgag aagacatgtt
    26521 cgcgtcctgt gcaggttata tggttgttaa cttcttgtca gagtttatgt cggccccgct
    26581 gtggttatct ttaaccaatt aatttgattt agatcgcaat ttgcgattaa agcacaaatc
    26641 taattccttg atttaaaata ctttcactct gttactatac gaaaacgtta attatcttgc
    26701 ccaaaaatca ggcaattatt gccctgaaag cgtgcatttg cgcagcaatc atcaaatcca
    26761 tacccgacaa aaaccgtgca aaataacagc aaatgttagc aaatagcatt aaatattgcg
    26821 caaatgataa ccacatttgt gtttatcccg attttcgcga tcgcagccag agtggcgcaa
    26881 tccctgcaat acttaaatcg gtatcatgtg atacgcgagc ctccggagca tattttgaac
    26941 aggttacctt cgagcgcatc agctttagcg tgcagcgccc acgccctgaa tctcattgaa
    27001 aagcgaacgt tggatcatga ggagatgaaa gctcttaacc gggaggtgat tgaatacttc
    27061 aaagagcatg tcaatccggg gtttttagag tatcgcaaat ctgttaccgc cggcggggat
    27121 tacggagccg tagagtggca agcgggaggt ttaaatacgc ttgtcgacac ccagggacag
    27181 gagtttatcg actgcctggg aggttttgga attttcaacg tggggcaccg taatccagtt
    27241 gtggtttccg ccgtacagaa tcaacttgcg aaacaaccgc ttcacagcca ggagctgctt
    27301 gatccgttac gggcgatgct ggcgaaaacc cttgcggcgc taacgcccgg taaactgaaa
    27361 tacagcttct tctgtaatag cggcaccgaa tccgttgaag cggcgctgaa gctggcgaag
    27421 gcttatcagt caccgcgcgg caagtttact tttattgcca ccagcggcgc gttccacggt
    27481 aaatcacttg gcgcgctgtc ggccacggcg aaatcgacct tccgcaaacc gtttatgcca
    27541 ctgctgccag ggttccgtca tgtaccgttt ggcaatatcg aagccatgcg cacggctctt
    27601 aacgagtgca aaaaaaccgg tgatgatgtt gctgcggtga tcctcgaacc gattcagggc
    27661 gaaggtggcg taattctgcc gccgccaggc tatctcaccg ccgtgcgtaa gctgtgcgat
    27721 gagttcggcg cactgatgat cctcgatgaa gtacaaacgg gcatggggcg cacgggcaag
    27781 atgttcgcct gcgagcatga gaacgtacag ccggatatcc tttgtctggc aaaagcgctc
    27841 ggtggcggcg tgatgccgat tggcgcaacc atcgccactg aagaggtgtt ctcagtcctg
    27901 ttcgacaatc cgttcctgca taccaccacc tttggcggca acccgctggc ctgtgcggcg
    27961 gcgctggcga ccatcaatgt gttgctggag cagaacttac cggctcaggc tgaacaaaaa
    28021 ggcgatatgt tgctggacgg tttccgtcaa ctggcgcggg aatatcccga tctggtacag
    28081 gaagcgcgtg gtaaagggat gttgatggcg attgagtttg ttgataacga aatcggctat
    28141 aactttgcca gcgagatgtt ccgccagcgc gtcctggtgg ccggaacact caacaacgct
    28201 aaaacgatcc gcattgaacc gccactgaca ctgaccattg aacagtgtga actggtgatc
    28261 aaagcggcgc gtaaggcgct ggcggccatg cgagtcagtg tcgaagaagc gtaatatcaa
    28321 atcggatggc gattcgacgt cgccatccga tctttttata cgatacgtac gcccgcaggc
    28381 atcatccgct ccggcgttaa cagcacgctt tcactgccgt catcggtttc cgcacacaac
    28441 agcatgcatt ctgacgtttc accgcgcatt ttcgcttttt gcagattgca taagaccacc
    28501 actgttttcc ccatcagctc ttcttcgctg tagtacggca ccaggctggt cacggtttgc
    28561 agcgtttttt cacccacatc aacctgtacg atgtacagct tgtcggcgtt ttcatggcgt
    28621 ttcacttcca caatctttcc gacacgcatt tccagacgtg caaaatcagc ataagccacg
    28681 gtttccattg tttcctccct tcgagtaaaa ttttactaaa ctatagcaaa agtttttctc
    28741 gatcctgtag gctaaaaatg gagaatgcag gcgtgatcac attcgtgagc cgctgtgtta
    28801 ccgttacagc gtcaaagaaa cgcgctttat ttactgaaaa caggtgaccc gataagcact
    28861 tcctctacaa tggaggcgca catcagggaa agtaaaaaag gtaaacatgg caacactaaa
    28921 agacatcgca atcgaagctg gcgtatccct ggcgacagta tccagggtct tgaatgacga
    28981 tccgacattg aatgtgaaag aagagacgaa acatcgcatt ctcgagatcg ccgaaaagct
    29041 ggagtacaag accagtagcg cccgtaaact ccagacaggc gcagtcaacc aacaccatat
    29101 tctggctatc tacagctacc aacaggagct ggagatcaac gatccttact atctggcgat
    29161 ccgccacggc attgaaactc agtgcgaaaa gctggcgatc gaactgacca actgttatga
    29221 acacaacggc ttaccagaca ttaaaaacgt caccggtatt ttaattgtcg gcaaacccac
    29281 gcccgccctg cgcgccgctg ccagcgcatt gaccgacaat atctgtttta tcgactttca
    29341 cgaacccggc agcggttacg atgcggtgga tatcgatctg gcacgcatca gtaaagaaat
    29401 catcgatttc tatatcgatc agggtgttaa tcgtattggt tttattggcg gtgaagactt
    29461 acccggcaag gcggatattc gtgaggtcgc ctttgcggaa tatggtcgtc tgaaacacgt
    29521 ggtacgcgaa gaggatatct ggcgcggcgg tttttccagt tcgtcgggct atgaactggc
    29581 aaaacagatg ctggcgcggg aagactatcc gaaggcactg tttgttgctt ccgattccat
    29641 tgctatcggc gtactgcggg caattcatga acgtggcctg aacatcccac aggatatttc
    29701 gcttatcagc gttaacgata tccccaccgc gcgatttacc tttccgccgc tctccaccgt
    29761 gcgcatccat tccgagatga tgggaagtca gggcgttaac ctggtgtatg aaaaagcccg
    29821 cgatggtcgc gcgctgccgc tgttggtctt cgttcccagc aaattaaaac tgcgcggcac
    29881 gacccgttaa atccccttgc acgctgtccg gcaatcgttt ttgccggaca gtgctgccgt
    29941 ttattttcgt gatccagtta aagtaaatgc atttatccgc tactttttag taaaattttt
    30001 actaaactcc ccagcaatta cacaaactac catcaccata aatggttccg atttctctct
    30061 accgggaggc cctatgaatc gctgggaaaa cattcagctc acccacgaaa accgacttgc
    30121 gccgcgtgcg tacttttttt catatgattc tgttgcgcag gctcgtactt ttgcccgtga
    30181 aaccagcagc ctgtttctgc ccttaagcgg tcagtggaat ttccactttt ttgaccatcc
    30241 gctgcaagtg ccagaagcct tcacctctga gttaatggct gactgggggc atattaccgt
    30301 ccccgccatg tggcaaatgg aaggtcacgg caaactgcaa tataccgacg aaggttttcc
    30361 gttccccatc gatgtgccgt ttgtccccag cgataaccca accggtgcct atcaacgcat
    30421 tttcaccctc agcgacggct ggcagggtaa acagacgctg atcaaatttg acggtgtcga
    30481 aacctatttt gaagtctacg ttaacggtca gtatgtgggg ttcagcaaag gcagtcgcct
    30541 gaccgcagag tttgacatca gcgcgatggt taaaaccggc gacaacctgt tgtgtgtgcg
    30601 cgtgatgcaa tgggcggact ctacctacgt ggaagaccag gatatgtggt ggtcggcggg
    30661 gattttccgc gatgtttatc tgatcggaaa acaactcacg catattaacg atttcaccgt
    30721 tcgtaccgac tttgacgaag cctattgcga tgccacgctt tcctgcgaag tggtgctgga
    30781 aaatctcgcc acctcccctg tcgtcacgac gctggaatat accctgttcg acggcgaacg
    30841 cgtggtgcac agcagcgcca ttgatcatct ggcaattgaa aaactgacca gcgcccgctt
    30901 tgcttttact gtcgaacagc cgcagcaatg gtcagcagaa tccccttatc tttaccatct
    30961 ggtcatgacg ctgaaagacg ccgacggcaa cgttctggaa gtggtgccac aacgcgttgg
    31021 cttccgtgat atcaaagtgc gcgacggtct gttctggatc aataaccgtt atgtgatgct
    31081 gcacggcgtc aaccgccacg acaacgatca tcgcaaaggc cgcgccgttg ggatggatcg
    31141 cgtcgagaaa gatctccagt tgatgaagca gcacaacatc aactccgtgc gtaccgctca
    31201 ctacccgaac gatccacgtt tttacgaact gtgcgatatc tacggcctgt ttgtgatggc
    31261 ggaaaccgac gtcgaatcgc acggctttgc taatgtcggc gatatcagcc gtattaccga
    31321 cgatccgcag tgggaaaaag tctacgtcga gcgcattgtt cgccatattc acgcgcagaa
    31381 aaaccatccg tcgatcatca tctggtcgct gggcaatgaa tccggctatg gctgtaacat
    31441 ccgcgcgatg taccatgcgg cgaaggcgct ggatgacacg cgactggtgc attacgaaga
    31501 agatcgcgat gctgaagtgg tcgatattat ttccaccatg tacacccgcg tgcctctgat
    31561 gaatgagttt ggtgaatacc cgcatccgaa gccgcgcatc atctgtgaat atgctcatgc
    31621 gatggggaac ggaccaggcg ggctgacgga gtaccagaac gtcttctata agcacgattg
    31681 cattcaggga cattatgtct gggaatggtg cgaccacggg atccaggcgc aggatgacaa
    31741 cggcaacgtc tggtacaaat tcggcggcga ctacggcgac tatcccaaca actataactt
    31801 ctgtcttgat ggtttgatct attccgatca gacgccggga ccaggcctga aagagtacaa
    31861 acaggttatt gcgccggtaa aaatccacgc gctggatctg gctcgcggcg agttgaaagt
    31921 cgaaaataaa ctgtggttta ccacgctaga tgactacacc ctgcacgcag aggtgcgcgc
    31981 cgaaggtgaa acactcgcga cgcaacagat taaactgcgc gacgttgcgc cgaacagcga
    32041 agccccctta cagatcacgc tgccgcagct ggacgcccgc gaagcgttcc tcaacattac
    32101 agtgaccaaa gattcccgca cccgctacag cgaagccggg cattctatcg ccacttatca
    32161 gttcccgctg aaggaaaaca ccgcgcagcc tgtacctttc gcgccaaata atgcgcgtcc
    32221 gctgacgctg gaagacgatc gtttgagctg cactgttcgc ggctacaact tcgcgatcac
    32281 cttctcaaaa atgagtggca aaccgacatc ctggcaggtg aatggcgagt cgctgctgac
    32341 ccgcgagcca aagatcaact tcttcaagcc gatgatcgac aaccataagc aggagtacga
    32401 agggctgtgg cagccaaatc atttgcagat catgcaggag catctgcgcg attttgtcgt
    32461 tgagcaaagt gatgatgaag tgttgctagt cagccgcacg gttattgccc caccggtgtt
    32521 tgacttcggg atgcgctgca cctacatctg gcgcatcgct gccgatggcc aggttaacgt
    32581 ggcgctttcc ggcgaacgtt acggcgacta tccgcacatc attccgtgca tcggtttcac
    32641 catggggatt aacggcgaat acgaccaggt agcatattac ggacgtggac cgggcgaaaa
    32701 ctacgccgac agccagcagg ctaacatcat cgatatctgg cgcagcaccg tcgatgccat
    32761 gttcgagaac tatcccttcc cgcagaacaa cggtaaccgt cagcatgtcc gctggacggc
    32821 actgactaac cgccacggca acggtctgct ggtggttccg cagcgaccaa ttaacttcag
    32881 cgcctggcac tatacccagg aaaacatcca cgctgcacag cactgtaacg agctgcagcg
    32941 cagtgatgac atcaccctga atctcgacca ccagttgcct ggcctcggct ctaactcctg
    33001 gggcagcgag gtgctggact cctggcgcgt ctggttccgt gacttcagct acggctttac
    33061 gttgctgccg gtttctggcg gagaagctac cgcgcaaagc ctggcgtcgt atgagttcgg
    33121 cgcagggttc ttttccacga atttgcacag cgagaataag caatgaggat catcgataac
    33181 ttagaacagt tccgccagat ttacgcctct ggcaagaagt ggcaacgatg cgttgaagcg
    33241 attgaaaata tcgacaacat tcagcctggc gtcgcccact ccatcggtga ctcattgact
    33301 taccgcgtgg agacggactc cgcgaccgat gcgctattta ccgggcatcg acgctatttt
    33361 gaagtgcatt actacctgca agggctgcaa aaaattgaat atgcgccgaa agagacatta
    33421 caggtagtgg aatattatcg tgatgaaact gaccgtgaat atttaaaagg ctgcggagaa
    33481 accgtagagg tccacgaagg gcaaatcgtt atttgcgata tccatgaagc gtatcggttt
    33541 atctgcaata acgcggtcaa aaaagtggtt ctcaaagtca ccatcgaaga tggttatttc
    33601 cataacaaat aacaactacg gcggcaaaag gagtttgccg ccaccgctac cctactcatt
    33661 ttcggagatg tgttatgtct gataccaaac gtaatacaat cggcaaattc ggcttgctct
    33721 cactgacttt tgccgccgtt tacagcttta acaacgtcat caataataat attgagcttg
    33781 gactggcctc ggcaccgatg tttttcctcg cgacgatttt ttattttatt cccttctgtc
    33841 tgatcatcgc agaatttgtt tcgttaaata aaaactcaga agccggtgtc tacgcgtggg
    33901 taaaaagttc gctgggcgga cgttgggcat ttattactgc ctatacctac tggttcgtaa
    33961 acctgttctt tttcacctcg ctgttaccgc gcgttattgc ttatgcttcg tatgccttcc
    34021 tcggctacga atatattatg acgccggttg ccaccaccat tatcagtatg gtgctgttcg
    34081 ccttctccac ctgggtttcc accaacgggg cgaaaatgct ggggccaatt acctccgtca
    34141 cttcaacgct gatgctgctg ttaacgctct cctacatttt gctggcagga acggcactgg
    34201 ttggcggcgt acagcctgct gaccccatca ccgttgacgc gatgatcccg aacttcaact
    34261 gggcgttcct cggcgttact acctggatct ttatggccgc aggtggcgcg gagtccgtcg
    34321 cggtgtacgt taacgacgtc aaaggcggtt cgaaatcgtt cgttaaagtg atcatcctcg
    34381 ccgggatttt tattggcgta ctgtactccg tctcctcagt actgattaac gtcttcgtca
    34441 gcagcaaaga gttgaagttt accggcggat cggtacaggt attccacggc atggcggcgt
    34501 attttggtct accggaagca ctgatgaatc gctttgtcgg tctggtgtcc tttaccgcga
    34561 tgttcggttc cctgctgatg tggacggcaa cgccggtgaa aattttcttc tccgaaatcc
    34621 cggaaggcat ctttggtaag aaaaccgtcg aactgaacga aaacggcgtt ccggcgcgcg
    34681 cagcgtggat ccagttcctg atcgtcatcc cgctgatgat tatcccgatg ctcggttcca
    34741 ataccgtgca ggatctgatg aatactatta ttaatatgac tgccgcagcg tccatgcttc
    34801 cgccgttatt catcatgctg gcttacctga atttacgcgc caaattagat cacctgccac
    34861 gcgatttccg tatgggctcc cgccgcaccg gtattatcgt tgtttcaatg ctgattgcga
    34921 tatttgccgt agggtttgtc gcttcgacat tcccgactgg cgcgaatatt ctgaccatca
    34981 ttttttataa cgtcggcggt attgttatct tcctcggctt tgcgtggtgg aaatacagta
    35041 aatatataaa gggattaacg gctgaagagc gccatattga ggcgacacca gccagcaatg
    35101 ttgattaagc agaataataa aaaatgagca ggcattttcc ctcttcctgg atggggagag
    35161 ggaaaatagt ttctgccttt atatttttat aattacaacg ataaaaggct gtactttttc
    35221 tttagctcat ggattaacac aatgaaatta atcactgcac catgcagagc attacttgct
    35281 ctgccgtttt gctacgcctt ttctgcggcc gctgatgaag cacgtccggc agaacatgac
    35341 gactcaaaaa cccccgcaat gacctcgaca tcttctcctt catttcgttt ttacggcgaa
    35401 ttaggggttg gtggatatat ggatttagag ggtgagaata aacataaata cagcgacggc
    35461 acctatattg aaggtggcct ggagatgaag tacggctcct ggttcggcct gatttacggc
    35521 gaaggctgga ccgtacaggc cgaccacgac ggtaatgcct gggtgcctga ccatagctgg
    35581 ggtggcttcg aaggtgggat taaccgtttc tatggcggtt atcgtaccaa tgatggcacc
    35641 gaaatcatgc tcagtctgcg tcaggattcc tcgctggatg acctgcaatg gtggggcgat
    35701 ttcacgcccg atctgggcta cgtcattccc aatacccgcg acattatgac tgcgctgaag
    35761 gtacagaact taagcggcaa ctttcgttat agcgttaccg ccacacctgc cggacatcat
    35821 gacgaaagca aagcctggct acattttggc aagtacgatc gctacgacga caaatacacc
    35881 tatccggcaa tgatgaacgg ttacatccag tatgacctta cagaaggtat cacctggatg
    35941 aatggtctgg aaatcaccga cggcacaggc caactctatc tcacgggcct gcttacccct
    36001 aactttgccg ctcgcgcctg gcaccatacc ggacgcgccg acgggctgga cgtaccggga
    36061 agtgaaagcg ggatgatggt gagcgccatg tatgaagcgt taaagggcgt ttatctctcc
    36121 accgcttaca cctacgccaa acatcgccct gaccacgctg acgatgaaac cacctctttc
    36181 atgcagtttg gtatctggta cgaatacggc ggcggacgtt tcgcaacggc ttttgatagc
    36241 cgcttctaca tgaaaaatgc ctctcacgat cccagcgacc aactcttcct gatgcaatat
    36301 ttttactggt aataaggaca gtaatctcat gaaaataaaa accattttaa cgccagtagc
    36361 ctgtgctctg ctgatgagct tttccgccca tgccgctaac gctgacaatt ataaaaacgt
    36421 gattaaccgt actggcgcgc cgcagtacat gaaggattac gattacgacg atcaccagcg
    36481 ttttaatccg tttttcgatc tcggagcctg gcatggtcat ctgttgccag acggcccgaa
    36541 caccatgggc ggctttccgg gcgttgcgct gctgacggaa gagtacatca actttatggc
    36601 cagcaatttc gaccgcctga ccgtctggca ggacggcaag aaagtcgact tcacgctgga
    36661 ggcatacagt attcccggcg cgctggtgca aaaactgaca gcaaaagatg tgcaggtcga
    36721 aatgactctt cgcttcgcca cgccgcgcac gtcactactg gaaaccaaaa tcaccagcaa
    36781 taaaccgctg gatctggtgt gggatggcga actgctggaa aaactggaag cgaaagaagg
    36841 gaaaccgctt tccgataaaa ccattgctgg cgaataccct gactatcagc gcaaaatcag
    36901 cgccacccgt gatggcctga aagtcacctt tggcaaagtg cgcgccacct gggatctgct
    36961 gacctccggt gaatcagaat atcaggtgca taaatccctg ccggtgcaga ctgaaatcaa
    37021 cggtaatcgc tttaccagta tggcgcatat caacggttcg acgacgctct acaccaccta
    37081 ttcccatctg ctgaccgctc aggaagttag caaagagcaa atgcagatcc gcgatattct
    37141 ggcgcgtccg gcgttttatc tcaccgcctc gcagcaacgc tgggaagagt atctgaagaa
    37201 agggttaacc aatccggatg ctacgccgga acagacgcgc gtcgcggtga aagccatcga
    37261 aacgctcaac ggtaactggc gatcgccggg cggtgcggtg aaatataaca ccgtcacgcc
    37321 gtcggtgacc gggcgctggt tctccggcaa tcagacctgg ccgtgggata cctggaagca
    37381 ggcgtttgcg atggcgcatt tcaatccgga tatcgccaaa gagaatatcc gcgcgatctt
    37441 ctcctggcag atccagccag gcgacagcgt gcgtccgcag gatgtgggct ttgtccccga
    37501 cctgatagcg tggaacctta gcaccgagcg tggcggcgat ggcggcaact ggaacgaacg
    37561 taacaccaaa cccagccttg ccgcctggtc ggtgatggaa gtgtacaacg tcacccagga
    37621 taaaacctgg ctggcagaga tgtacccgaa actggtggcc tatcacgact ggtggctacg
    37681 taaccgcgat cacaacggca acggcgtacc ggaatatggc gcaacccgcg ataaagccca
    37741 caatactgat aatggcgaga tgctgtttac agtgaaaaaa ggcaataaag aagagacgca
    37801 gtctgggctg aacaactacg cccgcgtggt ggagaaaggc cagtacgaca gtctggaaat
    37861 tccggcacag gttgcggcgt cgtgggaatc ggggcgtgat gatgccgccg tctttgggtt
    37921 tatcgacaaa gaacagttgg ataaatatgt cgctaacggc ggcaaacgta gcgactggac
    37981 ggtgaaattc gccgagaacc gcagtcagga cggaacgttg ctgggctact cgctattgca
    38041 ggagtcggtg gatcaggcca gctatatgta cagcgataac cattatctgg cggagatggc
    38101 gacgattctc ggtaagccgg aagaggccaa acgctatcgt cagttagcac aacagctcgc
    38161 ggactacatc aacacttgta tgttcgaccc gactacacag ttctactatg acgtgcgtat
    38221 tgaagataaa ccgctggcga acggctgcgc gggcaaaccg attgtcgagc gcggtaaagg
    38281 gccggaaggc tggtcaccgc tgtttaacgg tgcggcaacg caggccaacg ccgacgcggt
    38341 ggtgaaggtg atgctggatc cgaaagaatt caacaccttt gtcccgctgg ggacggcagc
    38401 gttaaccaac ccagcctttg gcgcagatat ctactggcgc gggcgcgtat gggtggatca
    38461 gttctggttt ggtctgaaag ggatggagcg ttacggttat cgcgatgatg ccctgaaact
    38521 ggcggatacg ttcttccggc acgccaaagg attaaccgcc gatggcccaa ttcaggagaa
    38581 ttacaacccg ctcactggcg cgcagcaagg cgcaccaaat ttctcctgga gtgccgcgca
    38641 tttgtatatg ttgtataacg atttttttcg taagcagtaa ttagcaacgg ctggcggatg
    38701 cggcgtaaac gccttatccg ccctacatgt gcgttccgta ggccggataa gacgcggcaa
    38761 gtgtcgcatc cggcaattgc accgcgccac tggcggatgc ggcgtaaacg ccttatccgc
    38821 cctacatgtg caatccccca ggccggataa gacgcgtcaa gcgtcgcatc cggcgttacc
    38881 accgggcgta ttctttttga atcccatcac aaaccccgca ctcccctttt cccttttctc
    38941 cggcgacggc taaattagaa ctcatccgac cacataacaa ttattttaca taatggacaa
    39001 ttttatgagc tacccgtcgc tgttcgcccc gctggattta ggttttacca cgttaaaaaa
    39061 ccgcgtgttg atgggctcaa tgcacaccgg gctggaggaa tacccgaacg gcgctgaacg
    39121 gctggcagcg ttttatgccg aacgcgcccg tcacggcgtg gcgttgattg tcagcggcgg
    39181 tatcgcacca gatttaacag gcgttggtat ggaaggcggt gcaatgctca atgacgccaa
    39241 ccagatccca caccatcgca ccattaccga agccgtacat caggaaggcg gcaaaatcgc
    39301 cctgcaaatt ttgcataccg ggcgctacag ctaccaaccg catctggtcg ctccctccgc
    39361 attgcaggcc cccatcaacc gtttcgttcc tcacgaactc acccatgaag agatcctgca
    39421 actgatcgac gatttcgccc actgcgcgca attggcacgg gaggcaggat acgacggtgc
    39481 agaggtgatg gggtccgaag ggtatttgat caacgaattt ctgacgctgc gcaccaatca
    39541 gcgtagtgac cagtggggcg gcgattaccg caaccggatg cggtttgccg tagaagtagt
    39601 acgtgcggtg cgcgaacgtg tcggcaacga cttcattatt atctaccgac tgtcgatgct
    39661 cgacctggtc gaaggcggcg gtacttttgc cgaaatggta gagttggcgc aggccattga
    39721 agcggcgggc gcgaccatta tcaacaccgg cattggctgg catgaagcac gtattccgac
    39781 cattgccacg cccgtgccgc gcggtgcctt tagctgggtc acgcgcaaac tgaaaggcca
    39841 cgtttcgctg ccgctggtga ccaccaaccg gattaacgat ccgcaggttg ccgacgatat
    39901 tctctcgcgc ggcgatgccg atatggtatc gatggcgcgg ccgtttcttg ctgatgcgga
    39961 actgctgtca aaagcgcaat cgggacgagc cgatgagatc aacacttgta ttggctgcaa
    40021 ccaggcctgt ctcgatcaga tcttcgttgg caaagtcacc tcgtgcctgg tgaatcctcg
    40081 cgcctgccat gaaaccaaaa tgccaatcct tcctgccgtg cagaaaaaaa atctggcggt
    40141 ggtcggtgcg ggacctgctg ggctggcgtt tgccattaac gcggcggcgc gtgggcatca
    40201 ggtaacattg tttgatgcgc acagcgagat cggcgggcag tttaatatcg ccaaacagat
    40261 ccccggcaaa gaggagtttt acgaaacgct gcgctattac cgccggatga tcgaagtgac
    40321 gggtgtgacg ctaaaactca atcacaccgt gacggcggat cagttacagg cgttcgatga
    40381 aacgatcctc gccagtggga tcgtgccgcg cgttccgcct atcgacggga tcgatcatcc
    40441 gaaggtattg agttatctcg atgtactgcg cgacaaagcg ccggtgggca acaaagttgc
    40501 catcatcggt tgtggcggaa ttggttttga tacggcgatg tatttaagtc agccgggcga
    40561 atccaccagc cagaatatcg ccgggttctg taatgagtgg ggaatcgaca gtagcctgca
    40621 acaggccgga ggtttgagtc cgcaaggcat gcaaatcccc cgtagcccac ggcagattgt
    40681 gatgctccag cgcaaagcca gcaaaccagg acaagggtta ggcaaaacca ccggctggat
    40741 ccatcgcacc accctgctct cacgcggcgt gaaaatgatc ccaggcgtaa gttaccagaa
    40801 gatcgacgat gacgggctgc atgtagtgat caacggcgaa acgcagatat tagcagtgga
    40861 caatgtggtg atctgcgcag ggcaagagcc aaaccgcgca ttggcgcaac cgctgattga
    40921 tagtgggaaa actgtgcatt taattggcgg ctgcgatgta gctatggagc tggacgcacg
    40981 gcgggcaatt gcccagggaa cacggctggc gctggagatt taaatcgttg tttgaagtgc
    41041 cggatgcgac gcttgccgcg tcttatccga cctgggggta atgtgctgaa cgtaggccgg
    41101 ataaggcgtt tacgccgcat ccggcaggta actcccaatc ttaccgacga cgccccagct
    41161 tcactgcttt cagcaccaca aatttattat tcgtggcgat ggtggtgcag ttgccgaaaa
    41221 tcttcttcag tttatggaag taatccaggt gacggttggc aacgatatac agctcgccgt
    41281 tgattttcag gcagcggcgg gcatggtgga acatctccca ggcgacgtta tcggtcagcg
    41341 catgttgctg atgaaacggc gggttgcaga gcacagcatt aaaacggaaa ggctccacgc
    41401 cggagagcgc gttgttgatc ataaactcgc agcgatccaa cgcctcgggc atgttggttt
    41461 ctacgtttaa ccggctggaa gcaaccgcca tcggcgattc atcgacaaac accactttcg
    41521 cctgcgggtt tttatcgagc aatgtcaggc caataacgcc attaccgcaa ccgagatcga
    41581 caatctcacc ttcgagattc tctggcagat gttgcataaa gaagcgcgcg ccaatatcca
    41641 gcccagtgcg ggagaaaaca ttcgcatggt tgtggatagt ccagtcagtg ccttccagct
    41701 tccagctaac ggtctgcggc gcatcggcca acggcggttc gttgaaggtg cagttaatca
    41761 ggcgcgcttt cttccatgcc agcgtggtgg tggtcggacc gagcactttt tcgaacaatt
    41821 ccagcgtgga agtgtgaatg tcacgggctt tggcaccggc aataatgcgc gtatctggcg
    41881 tgaccacttt gcgcagcgcg cgcagttgtt gttccagcaa tgccagtgtt ttcggcactt
    41941 tgatcagcac cacacccggc tgttgcgggt agtcggcggt gctgtcgagg aacttcacgc
    42001 tcgactcatc aatcccgttg aggcgtaaat tctcgcgcgt ggccagttca ctgatgtatg
    42061 agtcgccaat gctgtagggc ttatgttccg ccagcacaca gcttaacgca ccaaaagcat
    42121 cattcaggat caacaccggg ccgcggattt ctgtgtcgtc caactgttgc agcaaatatt
    42181 catccgccgc ttcccacgcc tgtagcgggt taacgtcatc cgtcgccgga aaacgttgta
    42241 gtgtcagtga acggaaaccg ttgtctaagt ggctcatcgg ccctcctgaa tgataaaatt
    42301 tcgacgttgc ccctgaaaag ggtgcgtgag tatacccttt ttctcttttt cgtgggcggt
    42361 tatgagcaat cttacttatc ttcagagtta tcccgagcag ctactttccc aggtgcgaac
    42421 gctgattaac gaacagcgtc tgggcgatgt gctggcaaaa cgttatccgg gaacacacga
    42481 ctacgccact gataaagccc tctggcaata tactcaggat cttaaaaatc agtttctgcg
    42541 caatgccccg ccgatcaata aagtgatgta tgacaacaag atccatgtgc tgaaaaacgc
    42601 gctcggctta catactgccg tttcgcgtgt gcagggcggc aagctgaaag caaaagcaga
    42661 gatccgcgtc gctaccgtgt ttcgcaacgc gccggaaccg tttttgcgca tgatcgtggt
    42721 gcacgagctg gcgcacctga aggagaaaga gcataacaaa gcgttttacc agttgtgctg
    42781 tcatatggaa ccgcagtacc accagcttga gttcgacacc cggctgtggc taacgcagtt
    42841 gtcgcttggg caggacaaaa tctgaaaatg atgtcaggcg ttattttttc ctcctttagc
    42901 gcctaaaatc gacctcctcc ctttcgttaa ggatgacgac catgctgcgc gcatttgccc
    42961 gccttcttct ccgtatttgt tttctcgccg gactcttaaa attggctgtt tattgttgct
    43021 tatagcagga gcaacccttt ttattgccga tcgcgttatg gtcaacgcca gccaacaact
    43081 gacctggagc gatgtcaacg ccgttccggc gcggaacgtg ggtttattgc tgggggcgag
    43141 gccgggtaat cgctacttta cccgacgtat tgataccgca gcagcgttgt accacgccgg
    43201 aaaagtgaaa tggctgttag ttagtggcga taacggacgt aaaaattacg atgaagcgtc
    43261 aggaatgcag caggccttga tcgctaaagg cgtaccagca aaagtgatct tctgcgacta
    43321 tgccggattc tcaacgcttg attcggtggt gcgtgccaaa aaggtgtttg gtgaaaacca
    43381 tatcaccatt atctcgcaag aatttcataa tcaacgcgcc atctggctgg caaagcaata
    43441 cggcatcgac gctatcggtt ttaacgcgcc agacctcaat atgaagcacg gtttttatac
    43501 tcaactgcgg gagaaactcg ctcgtgtcag cgcggtgatt gacgcaaaaa tcctccatcg
    43561 ccagccgaaa tatctcgggc cgttggttat gattgggccg tttagtgagc atggctgtcc
    43621 ggcgaaagaa taatgcgtat ctgcgcacgt tgaagatgaa aaaggcgtgc tacattgacg
    43681 agagaatccc tttatggagt atccacgcgt tatgatacgt ttcgctgtga ttggtacgaa
    43741 ctggatcact cgccagttcg tcgaggccgc ccatgagagc ggtaaataca agttaaccgc
    43801 cgtttattcc cgcagtcttg aacaggccca gcacttcgcc aatgattttt ctgtcgagca
    43861 tctgtttacg tcgctggaag cgatggcaga aagcgatgcc attgacgcgg tgtatattgc
    43921 cagcccgaat tccctgcact tttcccagac acaacttttc cttagtcaca aaattcatgt
    43981 gatttgcgag aaaccgctgg cgtcgaatct ggcggaagtg gatgccgcca ttgcctgtgc
    44041 gcgggaaaat caggtggtgc tgtttgaggc atttaaaacg gcctacctgc cgaactttca
    44101 tctgttacgc caggcgctgc cgaaagtcgg caaactgcgt aaagtctttt tcaactattg
    44161 ccagtattcc tcgcggtatc agcgttacct ggacggagag aatcccaaca cctttaatcc
    44221 gacattctct aacggttcaa ttatggatat cggcttttac tgcctggcgt cggcggtggc
    44281 attattcggt gaaccgaaaa gcgtgcaggc aaccgccagt ttgctggcaa gcggtgtgga
    44341 tgcccacggc gtggtggtga tggattacgg tgatttcagc gttacgttgc agcactcaaa
    44401 agtgagcgat tctgtcctgg caagcgagat tcagggcgaa gcgggatcgc tggtgattga
    44461 aaaactgtct gaatgccaga aagtttgctt tgtaccgcgt ggcagccaga tgcaggatct
    44521 cacccagcca cagcatatta ataccatgct ctacgaagca gagctgttcg ctaccctggt
    44581 ggatgagcat ctggtgaatc atccagggct ggcggtcagt cgtatcaccg ccaaactgct
    44641 gactgagatc cgccgccaga ctggggtgat ttttccggca gataacgtaa aactataatt
    44701 gccaaagtaa aacagtgtaa aaggtatgta acagaccatt gactgactga atggtctgtc
    44761 atactttgtt acctgcaaag gggagtaact tcattgtcgg tcgatcgtca ttacgatgtg
    44821 tgaaaaacca catccggtca ccgggcaacc cgaaaggaat acgcagacgt attccttttt
    44881 tgttgtaagt gagaccttgc cggaaggcga ggtctatgca taaaaagcag cggctgatgt
    44941 cttccgacgt tggccgtttt tttatgtgta aggaacttct atgaatactg tcggcacgcc
    45001 gttgctatgg ggcggattcg ctgttgttgt caccattatg ctggctatcg acctgttgtt
    45061 gcaggggcgt cgtggggcac atgccatgac catgaaacag gctgcggcct ggtcgctggt
    45121 ctgggtgacg ctgtcgttac tgtttaacgc cgctttctgg tggtatctgg tgcaaaccga
    45181 aggtcgcgcc gttgccgatc cacaggcact ggcctttctc acgggttatc tgattgagaa
    45241 atcgctggcg gtcgataacg tctttgtctg gctgatgttg ttcagctatt tctctgttcc
    45301 ggcggcatta caacgccgcg tgctggtgta tggcgtactc ggggcgattg ttctgcgtac
    45361 catcatgatc ttcactggta gctggctgat ttcacagttc gactggatct tgtatatctt
    45421 cggtgccttc ctgctgttta ccggcgtgaa gatggcgctc gcccatgaag atgaatcagg
    45481 cattggcgac aagccgctgg tgcgctggct acgcggtcat ttgcgcatga ccgacaccat
    45541 cgacaacgag catttctttg tgcgtaagaa tggtttgttg tatgccacgc cgctgatgct
    45601 ggtgctgatt ctggtggagt tgagcgacgt gattttcgcc gtggatagta ttccggctat
    45661 cttcgccgtg accaccgatc cgtttattgt gctgacctca aacctgtttg cgatcctcgg
    45721 cctgcgtgcg atgtatttcc tgctggcagg tgtggcagag cgtttctcga tgctcaaata
    45781 tggtctggcg gtgattctgg tgtttatcgg tatcaagatg ctgattgtcg acttctacca
    45841 tattcctatc gctgtctcgc tgggcgtggt gtttggcatt ctggtgatga cgtttattat
    45901 caacgcctgg gtgaattatc ggcatgataa gcagcgggta gaataatttt taatctgcct
    45961 aagccgtgta ctctgtcatc aacatgagca ccgttttctc cctctcccca aaaggggcga
    46021 ggggactgac cgagtacttt tttgacgttg tcatcagtct ggaagccgca cgttgggctt
    46081 tattttcatg tcaaacaaat gtaaccatta agttgcgaaa tatgacttct ctttaaaatc
    46141 cagcatattt cgcttcccga agctgtaact ttccttatac tcgaccttgc aaacactttg
    46201 ttacatcctg aaagatgcgt cgacagaacg caccagggat gtgcgacaac acaatgaaag
    46261 gatcgaaaaa tgactacgca acgttcaccg gggctattcc ggcgtctggc tcatggcagc
    46321 ctggtaaaac aaatcctggc cggccttatt ctggggattc ttctggcatg gatctcaaaa
    46381 cccgcggcgg aagctgttgg tctgttaggt actttgttcg tcggcgcact gaaagccgtt
    46441 gcccccatcc tggtgttgat gctggtaatg gcatctattg ctaaccacca gcacgggcag
    46501 aaaaccaata tccgccctat tttgttcctc tatctgctgg gcaccttctc tgctgctctg
    46561 gccgcagtaa tcttcagttt tgccttccct tctaccctac acttgtccag tagcgcgggt
    46621 gatatttcgc cgccgtcagg cattgtagaa gtgatgcgcg ggctggtaat gagcatggtt
    46681 tccaacccca ttgatgcgct gctgaaaggt aactacatcg ggatcctggt gtgggcaatt
    46741 ggcctcggtt tcgcactgcg tcacggtaac gaaaccacca aaaacctggt caacgatatg
    46801 tcgaatgccg tcacctttat ggtgaaactg gtgattcact tcgcaccgat cggtattttt
    46861 ggtctggttt cttctaccct ggcaaccacc ggtttctcca cactgtgggg ctacgcgcaa
    46921 ctgctggtgg tactggttgg ctgtatgctg ctggtggcgc tggtggttaa cccactgctg
    46981 gtatggtgga aaattcgtcg taacccgttc ccgctggtgc tgctgtgcct gcgcgaaagc
    47041 ggcgtgtatg ccttcttcac ccgcagctct gcggcgaaca ttccggtgaa tatggcgctg
    47101 tgtgaaaagc tgaatctgga tcgcgatacc tattccgttt ctattccgct gggagccacc
    47161 atcaatatgg cgggcgcagc aatcaccatt accgtgttga cgctggctgc ggttaatacg
    47221 ctgggtattc cggttgatct gcccacagcg ctgctgctga gcgtagtggc ttctctgtgt
    47281 gcctgtggcg catccggcgt ggcggggggg tctctgttgc tgatcccact ggcctgtaat
    47341 atgttcggta tttcgaacga tatcgccatg caggtggttg ctgtcggctt tattatcggc
    47401 gtattgcagg actcctgtga aaccgcgctg aactcgtcaa ctgacgtgct gttcactgcg
    47461 gcagcttgcc aggcggaaga cgatcgtctg gcaaatagcg ccctgcgtaa ctaatactta
    47521 gcccctttcg tctacggcgg aaggggtttt ctccacttta aacggatcaa ttccccttct
    47581 ctgcatacgc cagaaacgaa tgatattcag gccattcatc agcagaaaac tgccttcaat
    47641 catcgtgccg cctatcgacc ccgcccagaa gttgtgaatc acccagcaac acgttgaaaa
    47701 ccacattacg cagcgcatgg tcagcccttt acagcggaac agcgcccagg tactgacaat
    47761 cgtgccgatt accggcaata gttcgacagg atgatggaac ttcgcgaggc caattccgcc
    47821 agtcagcaca ataaaaatcg ccattaccca taagctgcgc gtgcgtaagg taatcaatgt
    47881 acgaatggca ttaaggatgg cactggcacc ggcaggatag gtgcccagaa gaaaaaaatg
    47941 tacgccaata acggcgctat agaccgaaag ttgctttttg aagcgacgtt cgtcacgatt
    48001 gaaaaatgtt gtgataccaa tcagaaaggc gatgacaccc acgccctggg ccagccaata
    48061 cgcggtcatg ataaatcctt agcaggtatg gaaaagcaaa cggcgcttca cattatgaaa
    48121 cgccgttttt tattaacaac tcatttcgac tttatagcgt tacgccgctt ttgaagatcg
    48181 ccagttcgcg gaagtcgtta cgctcgttac aggtttgctt accgttggca aactcaacga
    48241 tggtgtcgat aaattcttcc agcaactgcg gcatcgcttt accgtggatc agctgacccg
    48301 cgtcaaagtc gatccagtgt tttttcttcg ccgccagttc gctgttggtg gcgattttca
    48361 ccgtcggtac aaagccgccg tacggcgtgc cgcgaccagt actgaacagc accatatggc
    48421 agcccgcacc cgccagggcg ctggtcgcta cggcatcgtt acccggcgca cttaacaagt
    48481 tcagccccgg cgttttcaga cgctcgccgt aacgcagcac gtcaaccacg acactggaac
    48541 ccgctttctg ggtacagcca agcgatttat cttccagcgt ggtgataccg cctgctttgt
    48601 tccccggtga tgggttttcg tagatcggct ggtcatgggc aataaagtac tgcttgaagt
    48661 cattgaccat ggtgaccagt ttttcaaacg ttgcttcgtc gcgacaatgg tccatcagca
    48721 actgctctgc gccaaacatc tccggcactt cggtcagtac ggtagtaccg ccgttagcaa
    48781 tcacgtagtc agagaaacgc cccagcatcg ggttagcagt aataccagaa agaccgtcag
    48841 aaccaccgca ctccagacca aacttcagtt cgctgagttt gcccggctcg cgtttatcgt
    48901 tgcgcatcac gctatacagc tgatgcaaat gcgcgatccc ggcctcgatt tcatcatcct
    48961 gctgttggca gatcatgaaa tgaacgcgtt caggatcgat atcgcccaac gtttcacgga
    49021 atgcggcaac ctggttattt tcgcaaccca gaccaatcac cagcactgcg cccgcgttcg
    49081 ggtggcgcac catgttttgc agcatggtgc gagtattgat gtgatcgtcg cccagttgtg
    49141 agcagccgta ggtgtggctg aagaggaaca cgccgtcggt accttcagcg ttgttggtct
    49201 ctttcaggaa acgattttgg atctgccgcg cgatgccgtt gacacagccc acggttggca
    49261 ggatccacag ctcattacgc accccgacat cgccgttagc gcgacgatag atctgcactt
    49321 cacgatctgc cgcttgcgca ggcagatcct gaaaatcagg ttgatagcga tactgatcca
    49381 gatcgctcag attcgtgcgc gtattgtggg cgtgaacgtg ttcccccgcc gcaatatccg
    49441 ccaatgcata accaatcggc aggccatatt taatgacgtt ggcccctttt gcgatatccg
    49501 ttaacgcaaa tttatgtcca cgagcaacat cctggcgcag cgtaacagtc tggttatcga
    49561 cactgacttc tgtgccttca gccaaatccg ctaaagcaac cgcgacgtta tccagcgcat
    49621 ggatcttgat gtattgcata tcaaccccag accttagttc agttcaatgg cgaagtagtc
    49681 acgcgcattg ttaaagcaaa tatttttcac catctcgccc agcaggttga tgtccgccgg
    49741 tgcttcgcct gcttccaccc agcgaccgat catctggcac agaatgcggc ggaagtattc
    49801 gtgacgggtg tatgacagga agctacggct gtcagtcagc atgccgacaa agcggctcag
    49861 cagaccgagc tgcgccagtt gggtcatctg acgttccata ccgtctttct ggtcgttaaa
    49921 ccaccagccg gaaccgaact gcattttgcc cggcatacct tcgccctgga agttaccgat
    49981 catggtgccc agcacttcgt tatcgcgcgg gttcaggcag tagagaatgg ttttcggcag
    50041 caggttttct tcattctgct tgctcagcag cttagacagc tcttccgcca tcggacggtc
    50101 gttgatggag tcaaagccga catccggccc cagcagttta aactgacgca ggttgttatt
    50161 acgcagtgcg ccaatgtggt actgctgtac ccagccgcga cgtgcatatt cggcaccaag
    50221 gaacaccagc accgcagttt tgaactgtgc cacttcgtgc tcgctcaggg tttcgcctgc
    50281 cagacggcgc gcgaggatgc tgtccagttc cgcttcgttc gcttcagcaa acatcaccac
    50341 atccagcgcg tggtcagaca ctttacagcc gtgagcggcg aagtgatcca gacgtttagt
    50401 cagggcagtt tgcaggtcag caaagcggcg aatgtcggta tcggaaactt cgcccagctt
    50461 cgccatgtag tcgttaaagg tcgcctgttc aatgttaaag gctttgtccg gacgccagct
    50521 cggcagcact ttaatggtga aagagccgtc tttggcgatc tctgcgtgat gctccagaga
    50581 atcgatcggg tcatcggtgg tgccgaccat tttcacgttc atctgctgca tgatgccgcg
    50641 tgcagagaaa ttatcctgcg ccaggagttc gttacattcg ttccagattt catcggcagt
    50701 tgacggagaa agcaatttgc cagtgatacc aaacggacga cgcagttcga ggtgcgtcca
    50761 gtggtataac gggttgccga tagtgtgcgg aacagtcgcc gcccaggcgt caaatttttc
    50821 acggtcagac gcatcaccgg tgcacaaacg ctcggccaca ccgttggtac gcatagcgcg
    50881 ccatttgtag tgatcgccct tcaaccagat gtcatacagg tttttaaaac gatagtcttc
    50941 cgcaatctgc tgcggcggca aatggcaatg gtaatcgaaa atcggctggt cttttgcgta
    51001 gtcgtgatac agacggcggg caaattcagt atctaacagg aaatcttcag tcataaacgg
    51061 agtcattttc gtcttcctct caacgagtga gctagcttgc ttatggtgcg atgctgacaa
    51121 agttatcaca ccaatttcca gagtccgaag atattttcgt gagttagatc aataaacgta
    51181 gttaaaaaaa ttactctcaa agtggtaaat ctcgctgcag gccgcgccag tactggcctc
    51241 gccgtcgtca ggtaatgtcc ctacaaatat tcgcacattt gtgatggctc tcacctttta
    51301 aagttgtatg acaagttatc tttctgccgt cgcaaatcat aagtcgacgg aatgcaaatt
    51361 gccgattcat tcatttgtta gatgaatcgg gttaaccggt acggaagccg aattagcacg
    51421 aaactttcat ggcaacgttc ggggcgtgcc ggtttttttt cggttacccg gtcgtaacta
    51481 acatcttcag cctctggcgg gatgatggcc gcgcttcctg cggatataac aaaacgatga
    51541 ggttttacat gcgtaaaatt aaagggttac gttggtatat gatcgcactg gtgacgctcg
    51601 gcaccgtgct tggttacctg acgcgtaaca ctgtggcggc agctgcgcca actctgatgg
    51661 aagagttaaa catctccacc caacagtatt cctatatcat cgcagcgtat tctgctgctt
    51721 atacggtcat gcaaccggta gcgggttatg tactggatgt gctgggtacg aaaatcggtt
    51781 atgcaatgtt tgctgtactg tgggccgtgt tctgtggtgc aaccgcgctg gcaggtagtt
    51841 ggggtggcct ggcggttgct cgtggtgcgg tcggtgccgc ggaagccgcg atgattccgg
    51901 cgggtctgaa agccagctcc gaatggttcc cggcgaaaga gcgttccatc gcggtaggtt
    51961 actttaacgt aggttcttcg attggtgcga tgattgcgcc gccgctggtg gtatgggcaa
    52021 tcgtgatgca cagctggcag atggcattta tcatctccgg tgcgttgagc tttatctggg
    52081 cgatggcatg gctgattttc tacaagcatc cgcgcgacca gaaacatctg accgatgaag
    52141 aacgcgacta cattattaat ggtcaggaag cccagcacca ggttgacaca gcgaagaaaa
    52201 tgtccgttgg tcagatcctg cgtaaccgtc agttctgggg tatcgcgctg ccgcgattcc
    52261 tggcagaacc ggcatggggt acttttaacg cgtggatccc gctgttcatg tttaaagttt
    52321 acggctttaa cctgaaagag atcgcgatgt ttgcctggat gccgatgctg tttgctgacc
    52381 tcggttgtat cctcggtggt tacctgccgc cgctgttcca gcgttggttt ggtgtgaacc
    52441 tgatcgtttc ccgtaagatg gtcgtaacgc tgggcgctgt gctgatgatt ggcccgggta
    52501 tgatcggtct gttcaccaac ccgtatgtcg cgattatgct gctgtgtatc ggtggttttg
    52561 ctcaccaggc actgtctggc gcactgatta cgctctcttc tgacgtgttc ggtcgtaacg
    52621 aagtggcgac ggcgaacggt ttaaccggga tgtccgcatg gctggcaagt acgctgtttg
    52681 cactggtcgt cggtgcactg gctgacacca tcggcttcag cccgctgttc gcagttctgg
    52741 cagtgttcga cctgttaggt gcgctggtta tctggaccgt gttgcagaac aaaccggcga
    52801 ttgaggtggc gcaggaatca cataacgatc ccgcaccgca acattaaggc ttcgtgtaag
    52861 ttttagtttc ccttcaaagc cgccttctca ggcggctttt tcatcactgc gagtagagct
    52921 aaactcgcaa aagtggtata acaaatatag tctgccgtat catgcctgga gcgcatatgg
    52981 aaatcactga accacgccgt ttgtatcaac aacttgccgc tgacctgaaa gagcgcatcg
    53041 aacagggcgt ctatctggtg ggtgataaac tgcctgccga acgctttatt gccgatgaaa
    53101 aaaacgtcag ccgcaccgtg gttcgtgaag ccatcatcat gctggaagtt gaaggctatg
    53161 tcgaagtgcg taaaggttca ggtattcatg tggtttccaa ccagccgcgc catcagcagg
    53221 cggctgacaa taatatggaa ttcgccaatt acggtccgtt tgagttgctc caggctcgcc
    53281 agctcatcga aagtaatatt gccgagttcg cggcaactca ggtcacaaaa caggacatca
    53341 tgaaattgat ggccatccag gagcaggcac gcggcgaaca atgcttccgt gattccgagt
    53401 gggatttgca gttccatatt caggtagccc tggcgacgca gaactccgcc ctggcggcta
    53461 tcgttgaaaa aatgtggacc cagcgtagtc ataacccgta ctggaaaaaa ctgcacgaac
    53521 acattgatgc ccgtaccgtc gataactggt gtgatgacca cgatcaaatc ctcaaggcgc
    53581 tgattcgcaa agatcctcat gctgctaagc tggcaatgtg gcagcacctg gaaaacacca
    53641 agatcatgtt atttaacgaa accagcgacg atttcgagtt caatgccgac cgctatctgt
    53701 tcgccgaaaa cccggtggtg catctcgata cagccaccag cggcagtaaa tgaaattcct
    53761 gtccgacagg cgctacagtg cgcctgtttg tctcgtcggt aagcgaaaag tataaagtgt
    53821 cagcctgtgt aaatcctctc gcctccctcc cctgcattca gcaaaatcag actccacgga
    53881 catgtaattt tgataacgaa caacgttgac ctttgttaca attagattca atttgaattt
    53941 atgcttttga atgctttctt atctcacgat ttaacaggga atagttcagg ctgtgttgat
    54001 gtatcaaacc cgcagaacat accaagacag caataacatt gcggtagtgc atcatttaaa
    54061 accagcgtgg cgttaaccga ttcaccagga ataatgaatg gaacttttga cccaattgct
    54121 gcaagccctg tgggcgcagg attttgaaac cctggccaat ccgtcgatga ttggcatgtt
    54181 gtattttgtc ttgtttgtca ttttgttcct tgaaaacggc ttgcttccgg cggccttttt
    54241 accgggcgac agtttactgg tactggtcgg cgtattgatt gcgaaaggcg cgatgggcta
    54301 tccgcaaacg attctgctgc tgaccgttgc cgccagcctc ggttgctggg tcagctatat
    54361 tcaggggcga tggctgggca atacccgcac cgtacaaaac tggctatctc atttacccgc
    54421 gcattatcat caacgcgcac accatctttt tcataaacac ggtttatcgg cgctgttaat
    54481 tggtcgcttt attgcgtttg tcagaacact gctgccgacg attgccgggt tatcagggct
    54541 gaataacgcg cgctttcagt tcttcaactg gatgagtggt ctgctgtggg tgttgatcct
    54601 gacaactctg ggttacatgc tcggcaaaac gccggtattt ttaaagtacg aggaccagct
    54661 gatgtcatgc ctgatgctgc tcccggtggt gctgctggtg tttggcctgg caggttctct
    54721 ggtcgtgtta tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa tacctcgcat
    54781 gtcacttcgc cagctagcct ggtccggcgc tgttttactt ctggtcagca cactattgct
    54841 ggcctggtcc gcggttcgcc agcaagagtc tacgctggcg attcgtgccg ttcatcaagg
    54901 gacaacgatg ccagacggtt tttcaatctg gcatcacctt gacgctcacg gcattccttt
    54961 caaaagtatc acccccaaaa acgacactct gttaattacc tttgactcca gcgaccagag
    55021 cgccgccgca aaagcggtcc tcgacagaac attgccccat ggctacatca ttgcgcagca
    55081 ggacaataac agtcaggcta tgcagtggct gacccggttg cgggataact ctcatcgctt
    55141 cggataactt cctgttatcc gaaacagatc actcactttg gtgatttcac cgtaactgtc
    55201 tatgattaat gaggcggtgg ttaacactac cgcttcgttg tctggacaat cgttcctttg
    55261 taataggtcc gaatcacaat ggaaggttca agaatgaaat accgcatcgc tttagctgtt
    55321 tctctctttg ctcttagtgc cggtagttat gccactaccc tgtgtcagga aaaggagcaa
    55381 aatatcctta aggagatcag ctatgccgaa aaacaccaaa accagaatcg tattgacggt
    55441 ctgaataaag ccctgagtga agtccgggcc aactgttcag atagccagct gcgtgccgat
    55501 catcagaaga aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca agatttagcc
    55561 gaggcgaagc aaaaaggcga tgcagataag attgccaaac gcgaacggaa actggcagaa
    55621 gcgcaggaag agctgaaaaa gctgaaagcg cgcgactact aactcacaat agtcactact
    55681 tactcacctg gagaaaacta tgtcgaaaga acacactacg gaacatctgc gtgctgagtt
    55741 gaaatccctt tccgatacgc tggaagaggt gcttagctca tctggcgaga agtcaaaaga
    55801 agagttgagt aagattcgta gcaaagcgga gcaggcactg aaacagagcc gttatcgcct
    55861 gggtgaaacc ggtgatgcca ttgccaaaca aacccgtgtc gcggcggcgc gtgccgatga
    55921 gtatgtgcgt gaaaatccgt ggacgggcgt gggcattggc gcagcaatcg gtgtagtgct
    55981 cggcgttctg ctgtcgcgtc gttaattatg gcggacactc atcacgcgca agggcccggt
    56041 aaaagcgttc tgggcatcgg gcagcgaatt gtttccatca tggttgaaat ggtggagaca
    56101 cgtctgcggc tggcggtggt ggagctggaa gaggaaaaag cgaatctctt tcaactttta
    56161 ctgatgctgg gcctgacgat gcttttcgct gcatttggtc ttatgagcct gatggtgcta
    56221 gttatctggg cggttgatcc gcaatatcgc ctgaatgcga tgattgccac caccgtggtg
    56281 ttgctgctac tggcactgat tggcggcatc tggacgttac gtaaatcgcg taagtctacg
    56341 ttgctgcgcc acacgcgcca tgagctggca aacgatcggc agctgctcga ggaggagtcc
    56401 cgtgagcagt aaagtcgaac atgaacgacg taaggcgcaa ctgcttagcc agatccagca
    56461 acaacggctg gatctttccg ccagtcgtcg tgaatggctg gaggcaacag gcgcttacga
    56521 tcgtcgctgg aatatgctgt tgagtctgcg ctcctgggcg ctggttggca gtagcgtgat
    56581 ggctatctgg acgattcgcc atcctaatat gctggtccgc tgggccagac gcggttttgg
    56641 tgtatggagc gcctggcgtc tggtgaaaac aaccctcaag caacaacagc ttcgcggtta
    56701 acttcctctc tggccggaga aattccggcc ttatccctca aattttttga aaatatttga
    56761 cagttttcct tgctaacaat cacgcttcgt cacgtttatg attctctcca tcgacagcaa
    56821 cgacgctaat agcgcgccct tgcacaaaaa aacaatcagc agcctgagcg gcgcagtgga
    56881 gagtatgatg aaaaaattag aagatgttgg tgtactggta gcgcgtattt taatgccgat
    56941 tctgtttatt acggcaggct ggggaaaaat tactggctac gcgggtaccc aacaatatat
    57001 ggaagcaatg ggcgtccccg gctttatgct gccgctggtg attctgcttg agtttggtgg
    57061 tggtctggca atcctgttcg gtttcctgac acgtaccaca gccctgttta ctgcgggctt
    57121 tacgctgctg acggcatttt tatttcacag caactttgca gaaggcatga actcgctgat
    57181 gtttatgaaa aacctgacta tttctggcgg attcctgctg ctggcaatta ccggtccagg
    57241 cgcattcagc atcgaccgcc tgctgaataa aaagtggtaa gcacgcgcta tacttaacga
    57301 taaaaaacga gggggaagct cctcgttttt gctattggag gatagaaaaa tgggtcaact
    57361 gattgacggc gtctggcatg acacctggta cgataccaaa tccaccggcg gtaaatttca
    57421 acgttcagct tccgcatttc gtaactggct cactgccgat ggcgcgccag gccccactgg
    57481 caaaggcggt tttgccgcag agaaagagcg ttatcatctc tatgtttcac tcgcctgccc
    57541 gtgggcacac cgcacgctga tcatgcgtaa gctcaaagga ctggaaccgt ttatttccgt
    57601 ttccgtagtg aacccgctga tgctggaaaa cggctggacc tttgatgaca gttttccggg
    57661 agcaaccggc gacacactct atcaacatga atttctgtat cagctttatc tccacgccga
    57721 tccacactac agcggacgag tgactgttcc cgtgctctgg gacaaaaaaa accacaccat
    57781 cgttagcaac gaatcagcag aaatcatacg catgttcaat accgcgtttg atgcgctggg
    57841 tgcgaaagct ggagattact acccaccagc cctgcaaccg aaaattgacg aacttaacgg
    57901 ctggatttac gacaccgtta acaatggcgt gtataaagcc ggttttgcca ccagccagca
    57961 agcctacgac gaggcggtgg cgaaagtgtt tgaatcgctg gcgcgactgg aacagatttt
    58021 aggtcagcac cgttacctga ccggcaacca gctaaccgaa gccgatattc gcctgtggac
    58081 cacgctggtg cgttttgatc cagtgtatgt gacccacttc aagtgtgata agcaccgcat
    58141 cagcgattac ctgaatctgt atggcttcct gcgcgatatc taccagatgc cgggaattgc
    58201 cgaaacagtc aatttcgatc acatccgtaa tcattacttc cgcagccata agaccatcaa
    58261 ccctacgggg attatttcaa ttggtccgtg gcaggatctc gatgaaccgc atggacgaga
    58321 tgttcgcttc ggttaaaaaa aggcacctta cgaggtgcct ctgataatta gctaagtacg
    58381 tgttgttcat gtcggatgcg gcgtaaacgc cttatcaaac ctacaaaaca tttatattca
    58441 atatgttgca atgaccgcgc aggccagata agcgtagcgc atctggcaat tttgcgcttg
    58501 cccttatcct tcacagcgcc ttattaattc atcgcgataa tatttactgc catttcattc
    58561 acgtctattc ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat
    58621 ggactggtat ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa
    58681 agagtactgg atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctactgga
    58741 taaaatgtta ggctggcaac gtgccggtgg cgaaggcatc ctgacgacaa tctacggtat
    58801 tctggtgttt ttaccatggt gggcagttca gttccgccgc ctgcacgaca ccgaccgctc
    58861 ggcgtggtgg gcactgctat tcttaatccc gttcatcggc tggcttatta tcatcgtctt
    58921 taactgccag gcgggtacgc caggcgaaaa ccgctttgga ccagatccga aactggaaca
    58981 agaataataa ttaagcacct tgtaaaaggt gcttaatcga ttattttccg ctaaaaagtt
    59041 tgggaatttc ccgcagacac caggatttcg cctcccccat actgtcacga cgccaggcca
    59101 taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc ccttccgcaa
    59161 tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc agtaatgcct
    59221 gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc aactgtacgg
    59281 tcaacaccgg gcgctcacga gcggtatccg ccaccgcaat tccacgatat ttcacgcgcg
    59341 tcacttcaga taacggttcc ggctcctgat gaatcgggtg atccggcgcg gcaacataga
    59401 cgttcattaa ggtatagagt ttgcgcgagt tgatctccga cgaggaacga aaatgcatat
    59461 ccggcgcgat aacgatatcc gcccgcccct gctccaaccg ttcccacgcc cccgccagca
    59521 cttcggtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg attaacggga
    59581 aaaaggcagg tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt tcccaaccac
    59641 gcgcgagggc ttccgcatcg gtagtcagtt tatctgcggc ttccagcaaa acgcgccctc
    59701 gttccagcaa catcctcccg acattggtga atttggtgcg atggcccgag cggtcaaaca
    59761 gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg gaaggcacgc
    59821 gtcccagctc atccgccgcc gccgcaaaac tgccccggcg atcgatcgca tccataaccc
    59881 gtagtgcttc cagcgttaat gccctttctt tggccatttc gttctcattc aggaaatttg
    59941 aacataccgg gcagaatatc tggctaacaa tgtagcgtcc agccccttac cataaaagga
    60001 agtaaagaga ggtcaagaat tatgattact acccgaactg ccaggcagtg tggacaagca
    60061 gactacggat ggttgcaggc ccggtatact ttttcctttg gacactactt cgacccgaaa
    60121 ttgttaggct atgcctccct gcgcgtgctt aaccaggaag tgctggcccc aggtgccgcc
    60181 tttcagccgc gaacctatcc caaagtcgat attttaaatg tgattctgga tggagaagct
    60241 gagtatcgcg acagcgaagg caatcatgtt caggccagcg ctggtgaggc gttgctgctc
    60301 tctacccagc cgggtgtcag ctatagcgaa cacaatctca gcaaagacaa atcgttaacg
    60361 cgaatgcaac tttggctgga tgcctgccca caacgagaga atccgctgat tcaaaaacta
    60421 gcgcttaata tggacaagca gcaattaatc gcctcgccag aaggtgggat gggaagcctg
    60481 caattgcgcc agcaagtatg gctgcatcat attgtgctcg acaaaggcga aagtgcgaat
    60541 ttccagttgc atgggccacg cgcgtatttg caatcgattc acgggcaatt tcatgcactt
    60601 acgcatcatg aagagaaagc ggcgctgacc tgcggtgatg gggcgtttat tcgtgacgag
    60661 gctaacatta cgctggttgc cgattcccca ctgcgcgctt tgctgataga tttgcctgtc
    60721 tagttgtttt tacagggaga tgatgatgag taaaaaatcg gccaaaaagc gtcagccagt
    60781 gaagcccgtg gtggcgaaag aacctgctcg caccgccaaa aattttggct atgaagagat
    60841 gttgagcgag ctggaagcca tcgtcgcgga tgctgaaacg cgtttagccg aggatgaagc
    60901 taccgcgtaa agacaggtgc cggatgcggc gtaaacgcct tatccggcct acaaaaccca
    60961 acaacatcaa caaattgtgc aattcgtagg ccagataaga cgcgccagca tcgcatccgg
    61021 cacaataccc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
    61081 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
    61141 catcatgcgc cacaatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
    61201 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgatgcac
    61261 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatggttt
    61321 cataacgccc atccaccagc catgccatcc cggcagcggc ccccattgct gcggtcgttg
    61381 cggcacacag cgcagacaaa cgcggtaact ggttatggat gtaaatcgcg ctcaaatgcg
    61441 aaagcatcag cgcacgcgcc agccgttcat catccgctcc gaagtgttca gctaccacca
    61501 ccacgggcat tgttgcggtg atcccctggt tacccgagcc ggagttactc attgccggaa
    61561 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
    61621 agagatcttt cgccagcaaa ccgcgcgcgc actgtttttc cagcgtcgca ccaatatgca
    61681 gcccccagtt accgctcaaa ccttcctgcg ataacgcaca atttaacttc gcagaatcga
    61741 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
    61801 tcgtcctgga aagcaccgtc agcggcgact cttgctcgcc ctctgtcaca cacgcctgct
    61861 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
    61921 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
    61981 cgcaaggttc ctggatctta actgagactt tccccgccgc cagcagtgcc ttggcatcgg
    62041 aaattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttagca tttccaccta
    62101 acgctcccag cgccgccgca attggcagcc ccaccattcc tgtaccagga acggtgacac
    62161 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
    62221 ccagttcagc cgcagcaacc gccgccgcaa gcgccagtga aatcggttca gtacacccca
    62281 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
    62341 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaacg
    62401 gagaaacaca aagcaacaaa ccagtgacga taatcaggta caaagacatc cctttgtatt
    62461 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
    62521 aaatcgggct acagatagag gtgaagctca acaccggggc gttcagtacg atggcgctcc
    62581 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac gagattttcg ttaatcttct
    62641 cggcaggtat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
    62701 ctaaatagac gccaaagaat gcagtcatta cagcaaagat attgagaatg acgctgacca
    62761 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    62821 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    62881 cgtagaaaaa gacggtgaca aacaaaatac caaacgcgat attcattgcc cgcaatgctt
    62941 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    63001 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    63061 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    63121 acacgcccag cgccgcgacc accagcagct tggtcagcac catccccgtc gaaattttga
    63181 ataacaattt ctcgccgcgt gaggagatcg ccaccagaat gcaaatcagt accagaccat
    63241 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcagg taggaagcac
    63301 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    63361 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    63421 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    63481 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    63541 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    63601 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    63661 ccgccggggt cgaagcgtct gcaatgacgc atttattcga tgcaatttcc ataatttctc
    63721 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    63781 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgtgagat cagagaaaat
    63841 cttcacaatc aagactttta aatagcaatt gtgggtaata attttctatt aaattgagtt
    63901 aatagaaata attattctct atttgcggca gatcacaata aaaaagggtg cacattttgt
    63961 gcacccaaga atgatagctg acagcaatgt cagccgcaga ccactttaat agccagtcct
    64021 ccgcgtgatg tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg
    64081 atcactttat cgagtgaaac acgcggtgcc gaggtgcggc gcattgccat ccgcgcggcg
    64141 tttactgctt tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg
    64201 gcaaccggat cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat
    64261 acctgcgccg gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggct
    64321 acaccaatct ccccctgaca gccgacttcc gcgccagaga tggaggcgtt cattttatac
    64381 agcgcgccaa tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga
    64441 cgacggaact tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc
    64501 ggtgccgtta ccacgcgccc gccagctgcg ttttcttcac taaccgctag cgcgtacatg
    64561 ttgatccagt cgatgacatt catcggatcg ttagagatgt tatcgctgga aaccagttga
    64621 cgacgcagcg ctacggcacg gcgcggcaca ttgagcggac caggcagcac gccttcagta
    64681 ttcattccac gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca
    64741 atttccgctt tactgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg
    64801 ccgttgtaat cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacgggt
    64861 gtttcgacat cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca
    64921 gagtaatagg ttttgcttaa tagttcttcc tgtcccttcc aggcagtgat ccgcattccg
    64981 ttctcatggc gaggcaacat ttcgggatgg aagataatgc tctttgctac aggaaaatca
    65041 acaatatgcg cgccagaagc caccggcaac cgcccgctgc gcgtcactaa ctctataaat
    65101 gcagggatct catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgatgatg
    65161 gcgacatccg tggcatggcc tttgcccgtc agtgacaacg acccgtacag atcgaccaca
    65221 atatggctcg tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg
    65281 gcattcattg gccccacagt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg
    65341 aatgcactaa tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc
    65401 tagcgaccgt cccgctcacg aggccttacg cactacgtac tgcgatggct tcaatttcca
    65461 gcttcacatc tttcggcaaa cgcgcgacct gcacacagct ccgggtcggg taggtcgcct
    65521 gatgttcatc gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg
    65581 tgatgaacac ggtcatcttg atgatatcgc ccacactcag cccggcagca accacgatcg
    65641 ctttgacgtt ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg
    65701 tctgtgggca aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct
    65761 gaacataagg gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc
    65821 ctccggcgtc agagcgcctg ggtaaaggta cgtgaaataa catcctgttg ctgttcacgg
    65881 gtcagtgcgt taaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt
    65941 tccgggtgct cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt
    66001 tgaccgcctt cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg
    66061 gttttacgta ctggatcttc tttgcccagc gccgcaggga cgatggagaa ggtgtacgag
    66121 atcccatctt tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggcg
    66181 cctttgcgat cacgaccgtg catcgggtta gcgcccggcg cgaacggtgt tccggcgcgg
    66241 cgaccgtccg gcgtgttccc ggttttctga ccgtacacca cgttagaggt gatagtcaga
    66301 atcgattggg tcggcacagc attgcgatag gttggcaaag ctttaatttt cttcataaag
    66361 cgttcaacca gatcgcaggc aatgctgtct acgcgctcgt cgttgttgcc atactgcgga
    66421 tattcgccgt caatctcgaa atccaccgcc aggccgtttt catcacggat tggtttcaca
    66481 cgagcatatt tgatggcaga cagggagtcc gttgccaccg acaggcccgc gatgccgcac
    66541 gccatggtgc gatagacatc acgatcatgt agcgccatca gcgaagcttc gtagctgtac
    66601 ttgtcgtgca tgtagtgaat gatattcagc gcgctgatgt actgtaccgc cagccagtcc
    66661 atgaagtggt cgaggctatc catcactttg tcgtagtcca gcacgtcatc catcaacgga
    66721 gccgttttcg ggccaacctg aatcttcagc ttctcgtcca ttccgccgtt gattgcgtac
    66781 agtagcgttt tcgccaggtt agcgcgcgca ccgaagaact gcatctgctt accaatcacc
    66841 attgggctga cgcaacaggc aatcgcgtaa tcgtcgctat tgaagtcagt acgcatcaga
    66901 tcgtcgtttt catactgcaa agaggatgtg acgatagaca cctgcgcggc atattttttg
    66961 aaggcaatcg gcaaggcttc cgaccacaga atagtcaggt taggttccgg tgcaggcccc
    67021 atagtgtgca gggtgtgcaa atagcggaag gagtttttgg tcaccagggt tcgaccgtcc
    67081 agccccatcc cgccgatcac ttccgtcgcc cagatcgggt cgccggagaa cagcgaatca
    67141 aattccggcg tacgcaagaa gcgcaccata cggatcttca tgatgaagtg gtcgatcagt
    67201 tcctgtgcct gctgctcatt gagtacgcct gctttaaagt cgcgctcaat gtagatatcg
    67261 aggaacgatg ccgtgcgccc cagcgacatc gcgccgccat tttgcgattt cactgccgcc
    67321 aggtaagcga agtagagcca ctgcaccgct tcctgtgcat tctgcgccgg acgggagatg
    67381 tcaaagccat atttcgccgc catttcctga atctgcaaca gcgcacgacg atgttccgcc
    67441 agctcctcac gcagacggat cgtagcttcg agatcttcgc ctttttccag acgggactgg
    67501 agatcggcaa actgcagttc gcgttcacgt accagataac tgatgccgta aagcgccacg
    67561 cggcggtagt caccgatgat acgcccacga ccataaccat ctggtaaacc ggtcagtaca
    67621 ccagatttac ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg
    67681 gttttacgca gatcggtaaa gatgtattca aattcgctat ccatttcacg accatacgcg
    67741 tgaaatgaac ttctaatcat attaatgccg ccgaacggat gcagcgcacg ctttaatggc
    67801 gcatccgttt gcagacccac tattttttcc agcggctggt caatatatcc tgcatcatga
    67861 gcggtaattg tggtggcaat attggtatcg aaatccaatg gcgcgtgagt cgcattttcg
    67921 atacggatgc cttccatgac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg
    67981 agaaaagatt catcgccttc ataaggtgta tagttatgtt gaataaaatc gcggacatta
    68041 atttcgtttt tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg
    68101 ctggtatcaa tatctacctt catgaaaaat aatctctcta caatacttca actaaattat
    68161 gcaaattctg cgggcgcgtt cactttgcct aaatgaatgg catccaaagc aatcattttt
    68221 tcttcgttag tcggaattac ggcgcaaatg acacgcgcat tttcactgga aacaattcgc
    68281 tctccaaaag agttagagcg attattcatt tctgtatcaa tctctacgcc taatacagct
    68341 aaatgttcca tgaccagacg acgaattaaa cttgaattct ctcctattcc gccggtgaat
    68401 ataattccat ccaggcgatg taatgaagca gcgtgtccgg caatatgacg ggcaattcgg
    68461 tgaacaaagg ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc
    68521 agaacacgta aatccgaaga aaggccggaa atacctaata atcccgactc tttattcact
    68581 acgcgttcca ggtcccccag gctctggttg gtttggctgg cgacccagga catcgccccg
    68641 aagtcgacat caccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatg
    68701 gaagtgtcaa cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc
    68761 gccacaacca ggccggagtc atcttccgcc agattcagca gagaatgcgc gcgctgggaa
    68821 acatagcggt gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat
    68881 ttccacggca ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca
    68941 aataccgcca cctgagttac gcccgggaat aattgctgcg ccgattcaat accacttaaa
    69001 ttggcgtaat tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca
    69061 tcggtaataa tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt
    69121 aaggccacat tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca
    69181 ccttcgtagc tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca
    69241 ttttccgagt taataccgtc ggcaatacct gacattaata cttcacagtc gctggcattg
    69301 agtacggaaa acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca
    69361 ttcatctctt ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat
    69421 ggtcagcaga ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag
    69481 agacggcgct ttacggatgg catacatcgg caacaggcac agcagtgatg cgataatcgg
    69541 tgcgcccatg gcttcaatca ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt
    69601 ggagcccata atgaaaatca tgctgatagt attcagttta cccagcgaca ctttggtttt
    69661 gtcaccttta taaccgaact tcagaatcaa accattcagc ccttccagcg tccccagata
    69721 gtgaccgaag aaagatttga aaatagccac gagtgcgatg atggaagcgg catattccag
    69781 tgtaatcgca aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga
    69841 aagcactgga atattctgcg ctttcgcttc agccatattc gccggagaca gcgtaaacag
    69901 gcagctaaag gcaaagaaca tcaccactgc aaccatcagc atactggcgc gagagatgat
    69961 ttgtgaacat ttacgctcgg tgaagtcgcg accaaaatct ttctcgtact cttcacgttt
    70021 agaaacaacg aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat
    70081 ccccagccag acggtgatca ggataccgtc atgaccagtt aacgacagcg aaccgaggtc
    70141 aacctggtcg ataactgcag agttccagta agggatcagc gaaagagaaa tcagcaccag
    70201 gctggcgata aacggccata ccagatagct catcacttta accatcagat ccttaccaaa
    70261 ccagatgacg aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg
    70321 tgcaaagccg agctggtttt cccagaacgt cataaaggta ttggtaatgg taacgccata
    70381 aatccacaac agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt
    70441 tttaccaaaa tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag
    70501 acacagacgc gccagtgccc ggtggcaata aaacgcgatg gggtatgcta ataccaacat
    70561 cagaagaatc gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc
    70621 gccgattgcc gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga
    70681 ttgttttgtc tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat
    70741 taattaagcg tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc
    70801 ggaaataata ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa
    70861 tgcagcacat gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc
    70921 aatcatactg tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg
    70981 aacgatttcg taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc
    71041 ggtagttcgg tgcgtagtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt
    71101 ttcagactgt acgccaataa cacggatggt cgggttgata gatttaattg ccaccgcaat
    71161 accagcaatt aaaccgccgc cgccaattgg cacaatcacg ttatcgacat catagagatc
    71221 ttccataatt tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg
    71281 tgggataaaa atacggcctt ccatttcgac aatttcgctc actttagcaa tagtgtcgtt
    71341 gaagttatca ccatgcaaaa cgacttctgc ggagtagtcg cacgttgccg cgactttgga
    71401 tttcggcgca ccttttggca tcaccacttt accgtcaata cccagcatcg cgcaggagag
    71461 cgaaacccct tgcgcatggt tgcccgcaga gcaggccact acacctttac gtttttccgc
    71521 atcagtcagt gaacttaatt tattaaatgc gccacgaatt ttaaatgaac cggtacgctg
    71581 catgttttcg aatttgagga atatttcacc tttgcaacgt tcactaaaat agttggagcg
    71641 aggcatacct gttttataaa ttcgcccagc cagtcgttgt tttgcttcaa tgatgtcatc
    71701 aatagcaacc gggagatcgt atgtaatatg cattataaaa cctcttagcc tgaattaaat
    71761 atgtaggtaa ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaatt
    71821 aaaacaaata actaatcaac ttcaattaat tgccttcgtc tacacccatt ataagatgaa
    71881 tactctttgg ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac
    71941 cataccgcgg catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga
    72001 ttagaaccaa aaggtgatgt catatcacaa ggaattacag ttaagaaatc agcattgaga
    72061 acaagattat aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca
    72121 tttctttgta acgtagtaag tagttcgctg tagtacccca tattggtttg tggcaacacc
    72181 cactgttcgt tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg
    72241 gctaccagca caaactcgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt
    72301 tctgcgctta acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac
    72361 gaagacagtt gcgcttcata catagaaacc tgcgctttcg ggaacacctc tttgaacttg
    72421 ttgatcatcc ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc
    72481 accaccgcct cagaagacat accgcttatc tcattaacca tatttttcat ttcacgggta
    72541 atggattcgg aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt
    72601 ttccgcacca ctaactccac cccaaaataa tcttcaatat cgttaatgat tttactgacg
    72661 gccggttgag ttaaccctaa ctcttttgca gccgagccga tagaaccact tctaataact
    72721 tcctgaaaga ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac
    72781 gttaattacc tcattgacgg tatgaagtgt atcaaaatga aatgaacagg atatgtgcga
    72841 ccactcacaa attaactttc aatactttcc atggcatcgt tgccacaatt aaataccttt
    72901 aagtatcaac aagttaaagc atgaaaa
//
LOCUS       DADSZD010000002        65984 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.002, whole genome shotgun sequence.
ACCESSION   DADSZD010000002 DADSZD010000000
VERSION     DADSZD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.002"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1876
                     /locus_tag="J1Z28_000066"
     CDS             <1..1876
                     /locus_tag="J1Z28_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA7714941.1"
                     /translation="EGERKETGKYTSVVDIARSRVNQQNTENSAEKTGAVTVAVRREY
                     KQTWKTLDHELACALWPGDVNAGNIDGTIHRWATNEVIDKDREDWKRISASMRKQPEA
                     LGYDRQTIFGLVRERPIDIHKDPVALNKYISEYLTTKGVFEHEETDQSSADALQPSAA
                     QTAPVETAESDTQKNEILVEAEPSVEREGPFYFVFTDKGGEKYGRANKLSGLDKALAA
                     GATEISKEEYFARKNGTYTGLPQNVDTAEDSVQPEPVKVTADEVNKIMQAANISQPDA
                     DKLLAASRGEFVEGISDPNEPKWVKGIQSRNAEDQNQPGVKQNEPEAEQNSPDTQQNG
                     PEEQQPEPAVQQEVEKVCTACGQTGGGNCPDCGAVMGDATYQETFDGESLVEVQENAP
                     EEMEGTEDPHKENTDSDQYHASDNKTGETANPLIKVNGHREITSTSRLWHHLMIDLET
                     MGKNPDAPINAIAGKFFDPATGEMGPEFSKTIDLETAGGVIERDTIKWWLKQSREAQS
                     AILTDEIPLDDALLQFREFIDENSGEFFVQVWGNGATFDNVILRRSYERQGIPCPWRY
                     TNDRDVRTMVALGLVMDFDARTTIPFEGEHHNALHDTRYQAKYVSAIWQKLIPSQADF
                     "
     gene            1938..2207
                     /locus_tag="J1Z28_000067"
     CDS             1938..2207
                     /locus_tag="J1Z28_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA7714942.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKMGYIVPRPQKIRG
                     RWLIDRRAVFVGPSETGIAPEIHTDDDDALKEILTHVTEATKKQH"
     gene            2176..3294
                     /locus_tag="J1Z28_000068"
     CDS             2176..3294
                     /locus_tag="J1Z28_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7714943.1"
                     /translation="MSPRPRKNSTDIACLYEKFDRRTGKVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDSRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKDLEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLALEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKDVIDRC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWIKLVI"
     gene            3461..4255
                     /gene="potC"
                     /locus_tag="J1Z28_000069"
     CDS             3461..4255
                     /gene="potC"
                     /locus_tag="J1Z28_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBA7714944.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            4252..5298
                     /gene="potD"
                     /locus_tag="J1Z28_000070"
     CDS             4252..5298
                     /gene="potD"
                     /locus_tag="J1Z28_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBA7714945.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            5359..5820
                     /locus_tag="J1Z28_000071"
     CDS             5359..5820
                     /locus_tag="J1Z28_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HBA7714946.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNPWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRP"
     gene            5817..6605
                     /locus_tag="J1Z28_000072"
     CDS             5817..6605
                     /locus_tag="J1Z28_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HBA7714947.1"
                     /translation="MKKIIILLSLLILLPLTATSNPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAKLFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRFIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLWTSKR"
     gene            complement(6743..7564)
                     /gene="cobB"
                     /locus_tag="J1Z28_000073"
     CDS             complement(6743..7564)
                     /gene="cobB"
                     /locus_tag="J1Z28_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBA7714948.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(7580..8491)
                     /gene="nagK"
                     /locus_tag="J1Z28_000074"
     CDS             complement(7580..8491)
                     /gene="nagK"
                     /locus_tag="J1Z28_000074"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBA7714949.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQD
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(8520..9764)
                     /gene="lolE"
                     /locus_tag="J1Z28_000075"
     CDS             complement(8520..9764)
                     /gene="lolE"
                     /locus_tag="J1Z28_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBA7714950.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(9764..10465)
                     /gene="lolD"
                     /locus_tag="J1Z28_000076"
     CDS             complement(9764..10465)
                     /gene="lolD"
                     /locus_tag="J1Z28_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBA7714951.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(10458..11657)
                     /gene="lolC"
                     /locus_tag="J1Z28_000077"
     CDS             complement(10458..11657)
                     /gene="lolC"
                     /locus_tag="J1Z28_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBA7714952.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11919..12992
                     /locus_tag="J1Z28_000078"
     CDS             11919..12992
                     /locus_tag="J1Z28_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA7714953.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            13120..16566
                     /gene="mfd"
                     /locus_tag="J1Z28_000079"
     CDS             13120..16566
                     /gene="mfd"
                     /locus_tag="J1Z28_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBA7714954.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVDAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLESFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDTVRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWIRQFMRELEENA
                     IA"
     gene            16709..17671
                     /gene="ldtC"
                     /locus_tag="J1Z28_000080"
     CDS             16709..17671
                     /gene="ldtC"
                     /locus_tag="J1Z28_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBA7714955.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17753..18010)
                     /gene="bhsA"
                     /locus_tag="J1Z28_000081"
     CDS             complement(17753..18010)
                     /gene="bhsA"
                     /locus_tag="J1Z28_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBA7714956.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            18251..18883
                     /gene="comR"
                     /locus_tag="J1Z28_000082"
     CDS             18251..18883
                     /gene="comR"
                     /locus_tag="J1Z28_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBA7714957.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(18945..19484)
                     /locus_tag="J1Z28_000083"
     CDS             complement(18945..19484)
                     /locus_tag="J1Z28_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBA7714958.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
     gene            complement(19711..21015)
                     /locus_tag="J1Z28_000084"
     CDS             complement(19711..21015)
                     /locus_tag="J1Z28_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA7714959.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(21414..21956)
                     /locus_tag="J1Z28_000085"
     CDS             complement(21414..21956)
                     /locus_tag="J1Z28_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7714960.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(21979..23004)
                     /gene="nagZ"
                     /locus_tag="J1Z28_000086"
     CDS             complement(21979..23004)
                     /gene="nagZ"
                     /locus_tag="J1Z28_000086"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBA7714961.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSTYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISARLNQLHERW
                     QEEKAGH"
     gene            complement(23015..23839)
                     /gene="thiK"
                     /locus_tag="J1Z28_000087"
     CDS             complement(23015..23839)
                     /gene="thiK"
                     /locus_tag="J1Z28_000087"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBA7714962.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNRGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRL
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(23820..24461)
                     /gene="lpoB"
                     /locus_tag="J1Z28_000088"
     CDS             complement(23820..24461)
                     /gene="lpoB"
                     /locus_tag="J1Z28_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBA7714963.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(24475..24852)
                     /locus_tag="J1Z28_000089"
     CDS             complement(24475..24852)
                     /locus_tag="J1Z28_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBA7714964.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(24855..25214)
                     /gene="hinT"
                     /locus_tag="J1Z28_000090"
     CDS             complement(24855..25214)
                     /gene="hinT"
                     /locus_tag="J1Z28_000090"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBA7714965.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGCPLGPMLAHKGL"
     gene            25549..27738
                     /gene="fhuE"
                     /locus_tag="J1Z28_000091"
     CDS             25549..27738
                     /gene="fhuE"
                     /locus_tag="J1Z28_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBA7714966.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLTPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNEEGSIVYGAPRNFSITGTYQF"
     gene            complement(27798..29231)
                     /gene="ptsG"
                     /locus_tag="J1Z28_000092"
     CDS             complement(27798..29231)
                     /gene="ptsG"
                     /locus_tag="J1Z28_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBA7714967.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(29525..30322)
                     /locus_tag="J1Z28_000093"
     CDS             complement(29525..30322)
                     /locus_tag="J1Z28_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7714968.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(30333..31337)
                     /gene="holB"
                     /locus_tag="J1Z28_000094"
     CDS             complement(30333..31337)
                     /gene="holB"
                     /locus_tag="J1Z28_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBA7714969.1"
                     /translation="MRWYPWLRPDFEKLVASYQARRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQAALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(31334..31975)
                     /locus_tag="J1Z28_000095"
     CDS             complement(31334..31975)
                     /locus_tag="J1Z28_000095"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBA7714970.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTNWVKELDA"
     gene            complement(31965..32987)
                     /gene="yceG"
                     /locus_tag="J1Z28_000096"
     CDS             complement(31965..32987)
                     /gene="yceG"
                     /locus_tag="J1Z28_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBA7714971.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(32990..33799)
                     /gene="pabC"
                     /locus_tag="J1Z28_000097"
     CDS             complement(32990..33799)
                     /gene="pabC"
                     /locus_tag="J1Z28_000097"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBA7714972.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(33919..35160)
                     /gene="fabF"
                     /locus_tag="J1Z28_000098"
     CDS             complement(33919..35160)
                     /gene="fabF"
                     /locus_tag="J1Z28_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBA7714973.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(35247..35483)
                     /gene="acpP"
                     /locus_tag="J1Z28_000099"
     CDS             complement(35247..35483)
                     /gene="acpP"
                     /locus_tag="J1Z28_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA7714974.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(35694..36428)
                     /gene="fabG"
                     /locus_tag="J1Z28_000100"
     CDS             complement(35694..36428)
                     /gene="fabG"
                     /locus_tag="J1Z28_000100"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBA7714975.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(36441..37370)
                     /gene="fabD"
                     /locus_tag="J1Z28_000101"
     CDS             complement(36441..37370)
                     /gene="fabD"
                     /locus_tag="J1Z28_000101"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBA7714976.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(37386..38339)
                     /locus_tag="J1Z28_000102"
     CDS             complement(37386..38339)
                     /locus_tag="J1Z28_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBA7714977.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(38407..39477)
                     /gene="plsX"
                     /locus_tag="J1Z28_000103"
     CDS             complement(38407..39477)
                     /gene="plsX"
                     /locus_tag="J1Z28_000103"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBA7714978.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(39558..39731)
                     /gene="rpmF"
                     /locus_tag="J1Z28_000104"
     CDS             complement(39558..39731)
                     /gene="rpmF"
                     /locus_tag="J1Z28_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBA7714979.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(39783..40304)
                     /gene="yceD"
                     /locus_tag="J1Z28_000105"
     CDS             complement(39783..40304)
                     /gene="yceD"
                     /locus_tag="J1Z28_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBA7714980.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVITGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            40502..41086
                     /gene="yceF"
                     /locus_tag="J1Z28_000106"
     CDS             40502..41086
                     /gene="yceF"
                     /locus_tag="J1Z28_000106"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBA7714981.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASCYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(41198..42157)
                     /gene="rluC"
                     /locus_tag="J1Z28_000107"
     CDS             complement(41198..42157)
                     /gene="rluC"
                     /locus_tag="J1Z28_000107"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBA7714982.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSYVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            42730..45915
                     /gene="rne"
                     /locus_tag="J1Z28_000108"
     CDS             42730..45915
                     /gene="rne"
                     /locus_tag="J1Z28_000108"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBA7714983.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKSAPATPAAPA
                     QPGLLSRFFGALKALFSGGEETKPSEQPTPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     PRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQTPRRERSRR
                     RNDDKRQAQQEVKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQPEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAAEPVVSAPVVEEVAEVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVTAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(46110..47063)
                     /gene="flgL"
                     /locus_tag="J1Z28_000109"
     CDS             complement(46110..47063)
                     /gene="flgL"
                     /locus_tag="J1Z28_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-filament junction protein FlgL"
                     /protein_id="HBA7714984.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEADGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(47075..48718)
                     /gene="flgK"
                     /locus_tag="J1Z28_000110"
     CDS             complement(47075..48718)
                     /gene="flgK"
                     /locus_tag="J1Z28_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBA7714985.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSTADPSRTTVAYIDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSLTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(48784..49725)
                     /gene="flgJ"
                     /locus_tag="J1Z28_000111"
     CDS             complement(48784..49725)
                     /gene="flgJ"
                     /locus_tag="J1Z28_000111"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBA7714986.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGNSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(49725..50822)
                     /locus_tag="J1Z28_000112"
     CDS             complement(49725..50822)
                     /locus_tag="J1Z28_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBA7714987.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     HGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(50834..51532)
                     /gene="flgH"
                     /locus_tag="J1Z28_000113"
     CDS             complement(50834..51532)
                     /gene="flgH"
                     /locus_tag="J1Z28_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBA7714988.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(51585..52367)
                     /gene="flgG"
                     /locus_tag="J1Z28_000114"
     CDS             complement(51585..52367)
                     /gene="flgG"
                     /locus_tag="J1Z28_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBA7714989.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(52539..53294)
                     /locus_tag="J1Z28_000115"
     CDS             complement(52539..53294)
                     /locus_tag="J1Z28_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HBA7714990.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(53314..55239)
                     /gene="flgD"
                     /locus_tag="J1Z28_000116"
                     /pseudo
     CDS             complement(53314..55239)
                     /gene="flgD"
                     /locus_tag="J1Z28_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706995.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
     gene            complement(55251..55655)
                     /gene="flgC"
                     /locus_tag="J1Z28_000117"
     CDS             complement(55251..55655)
                     /gene="flgC"
                     /locus_tag="J1Z28_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBA7714991.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(55659..56075)
                     /gene="flgB"
                     /locus_tag="J1Z28_000118"
     CDS             complement(55659..56075)
                     /gene="flgB"
                     /locus_tag="J1Z28_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBA7714992.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPSAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            56230..56889
                     /gene="flgA"
                     /locus_tag="J1Z28_000119"
     CDS             56230..56889
                     /gene="flgA"
                     /locus_tag="J1Z28_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBA7714993.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            56965..57258
                     /gene="flgM"
                     /locus_tag="J1Z28_000120"
     CDS             56965..57258
                     /gene="flgM"
                     /locus_tag="J1Z28_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBA7714994.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            57263..57679
                     /gene="flgN"
                     /locus_tag="J1Z28_000121"
     CDS             57263..57679
                     /gene="flgN"
                     /locus_tag="J1Z28_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBA7714995.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLYMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNSANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(57719..59254)
                     /gene="murJ"
                     /locus_tag="J1Z28_000122"
     CDS             complement(57719..59254)
                     /gene="murJ"
                     /locus_tag="J1Z28_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBA7714996.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(59364..60287)
                     /locus_tag="J1Z28_000123"
     CDS             complement(59364..60287)
                     /locus_tag="J1Z28_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7714997.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTTSHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKKQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(60289..60936)
                     /locus_tag="J1Z28_000124"
     CDS             complement(60289..60936)
                     /locus_tag="J1Z28_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HBA7714998.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLASREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAADGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(60947..61531)
                     /gene="rimJ"
                     /locus_tag="J1Z28_000125"
     CDS             complement(60947..61531)
                     /gene="rimJ"
                     /locus_tag="J1Z28_000125"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBA7714999.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            61767..62975
                     /gene="mdtH"
                     /locus_tag="J1Z28_000126"
     CDS             61767..62975
                     /gene="mdtH"
                     /locus_tag="J1Z28_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBA7715000.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            63039..63686
                     /gene="grxB"
                     /locus_tag="J1Z28_000127"
     CDS             63039..63686
                     /gene="grxB"
                     /locus_tag="J1Z28_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBA7715001.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNSADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            63820..64380
                     /locus_tag="J1Z28_000128"
     CDS             63820..64380
                     /locus_tag="J1Z28_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7715002.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            64486..65532
                     /gene="pyrC"
                     /locus_tag="J1Z28_000129"
     CDS             64486..65532
                     /gene="pyrC"
                     /locus_tag="J1Z28_000129"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBA7715003.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            65606..65851
                     /gene="dinI"
                     /locus_tag="J1Z28_000130"
     CDS             65606..65851
                     /gene="dinI"
                     /locus_tag="J1Z28_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBA7715004.1"
                     /translation="MRIEVTIAKTSPLPAGAIHALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        16288 a        17577 c        16407 g        15712 t
ORIGIN      
        1 cgaaggtgaa cgcaaggaaa ccgggaaata cacatctgtc gttgatatcg cccgttccag
       61 agtaaaccaa cagaacactg aaaactctgc tgaaaaaacc ggggctgtca ctgttgccgt
      121 tcgccgagaa tacaaacaga catggaaaac actcgaccat gagctggcct gcgccctctg
      181 gcccggtgat gtgaatgcgg gaaatattga cggtaccatc catcgctggg cgacaaatga
      241 ggttatcgac aaggatcgcg aagactggaa gcgtatctca gcatcaatgc gcaaacagcc
      301 cgaagcactt ggctatgacc gtcagactat ttttggcctt gtccgtgaac gtccgatcga
      361 cattcacaaa gatcccgttg cactgaacaa atatatcagc gaatacctga caacaaaggg
      421 cgtgtttgag catgaagaaa cagaccagag ctctgctgat gctctccagc cgtcagcagc
      481 acaaactgct ccagtggaga cggcagaatc cgatactcaa aaaaatgaaa tcctggtgga
      541 agctgaacca tctgtagagc gtgaaggacc attttatttc gtctttaccg ataagggcgg
      601 ggaaaaatac ggcagggcaa acaaactttc tggtctggac aaggcgctgg ctgccggcgc
      661 taccgaaatc tcaaaagagg aatattttgc ccgaaaaaat ggcacatata cgggcttacc
      721 gcaaaatgta gataccgctg aagattccgt acaaccagag ccggtaaaag ttaccgctga
      781 cgaagtaaac aaaattatgc aggcagccaa tatcagccag cctgacgccg ataaattgct
      841 tgctgcatca cgtggtgaat ttgttgaagg gattagcgac cctaatgagc cgaaatgggt
      901 gaaagggatc cagagtcgca acgctgagga ccagaatcag cccggcgtga aacaaaatga
      961 gccagaagcg gaacaaaaca gcccggatac gcaacaaaac gggccagaag agcaacaacc
     1021 agaaccagca gtgcaacagg aagtggagaa agtttgcacc gcatgcggtc agaccggtgg
     1081 cggcaactgt cctgactgtg gtgcggtgat gggcgacgca acctaccagg aaacattcga
     1141 tggagagagc ctggttgaag ttcaggaaaa tgctccggag gaaatggaag gcactgaaga
     1201 tccgcacaag gagaacactg acagcgatca gtatcacgcc agcgataata aaactggcga
     1261 gacagcaaat cccttaatta aggtgaacgg tcatcgtgaa atcacatcca ccagcaggtt
     1321 gtggcaccat ctgatgattg accttgaaac aatgggaaaa aatcctgatg cgccaataaa
     1381 cgctatagcc ggtaagtttt ttgatccggc aaccggagag atggggccag aattcagcaa
     1441 aactatcgat ctggaaaccg caggcggggt catcgaacgg gacaccatta agtggtggct
     1501 gaaacagtca cgcgaagcac aatccgccat tctgaccgat gaaatcccgt tggatgatgc
     1561 actgctgcaa ttccgggaat ttatcgacga aaactccggt gaattttttg ttcaggtctg
     1621 gggtaacggt gcaactttcg acaacgtgat tttacgccgt tcatatgaac ggcaggggat
     1681 cccctgcccg tggcgttaca ccaatgatcg cgatgtaaga acgatggttg ctctgggact
     1741 ggtgatggat ttcgacgcaa gaacgactat tccattcgaa ggtgaacacc ataacgccct
     1801 gcacgatacg cgttaccagg caaaatacgt ttcagccatc tggcaaaaac tgatcccgag
     1861 tcaggctgat ttttaatgtt caaccgtcgc cagttgtagt tgctattctg caactggcgc
     1921 attccggagt gatggccatg agcgaacagt acctgataac gctcgacgag tggaaaccta
     1981 aacggttcag tctcccaata acaaacacta ccctggtgaa atacggaaaa atgggataca
     2041 tcgttccaag gccacaaaaa atccgtgggc gttggctgat agatcgccga gcagtatttg
     2101 tgggtcctag tgaaacagga attgcgccgg aaattcatac tgacgatgat gacgcactga
     2161 aggagatttt aactcatgtc accgaggcca cgaaaaaaca gcactgacat agcatgtctt
     2221 tacgaaaagt tcgatcgcag aactggcaag gtttactacc agtataaaaa tccagtgact
     2281 ggaaaatttc acggactcgg aacagacaaa ggcaaggcag aaaaaatcgc ttccacggcc
     2341 aatcaacgaa tagctgcagc agaagcagaa tatttcatgc gcaaaattga tgaaagtcca
     2401 tcagcaacaa aacgtcgggg tatcagatta aaagcatggg ttgatcgata tctgaaaata
     2461 caggactcac gactgaaaaa tggagatatt gcagctacaa cccacaaaga aaaggcgcga
     2521 atggctgcat acctggtttc ccgcctggga aatcacccat tgaaagacct ggaagtaaga
     2581 gactttgcat taatactgga tgaatggctg gataaagaca tggtcagcac agcgagagta
     2641 aatcgcggat tatgggttga tatttataaa gaagcacaac atgcagggga agttcctcct
     2701 ggatggaatc ctccggaggc tacccgtaaa ccgatcccta aagtaaccag agccaggctc
     2761 gccctggaag actggcaaaa aatttacaac gcaacgccgg aaaaacactt tatccgtaac
     2821 gcaatgcttc ttgcgattgt cactggtcag cgccgtgatg acatttgcca catgcgtttt
     2881 tcagatgtgt ggaacgaaca cttgcatatc acccagggaa aaaccggaat gcgtctggcg
     2941 ttaccgctta cactacgctg tgatgccatt gggataacgt taaaagacgt tattgataga
     3001 tgccgagaca gaatattaag tccatatcta atccatagtc ggcaccagaa acaaccaaag
     3061 ccgatgagta aagacaacct gagcgactac tttgccaaag cgcgggatct ggctggaata
     3121 attccaccag caggaaaaac accgcccaca tttcatgaac aacgctctct atcagaacgg
     3181 ctgtaccgtg cacagggtat cgatacaaaa acattactag gacataaagt ccaggcaacc
     3241 accgatcgct ataacgatac ccgaggtcag gaatggatta agttggttat ttgacaaaca
     3301 ctatatggaa tatccgtcac catctcagga aaaaagtgtt gtaaaatgcg tggccttgtt
     3361 ttggggaaaa gttttggaga gattttggag aaagggaaaa attgaataaa ttcaaaatcc
     3421 tgaaagtgct ccaccttatg aataagaagg tggaactcga atgatcggtc gactgcttcg
     3481 cggcggtttt atgaccgcta tctacgcgta cctgtatatc ccaatcatta ttttgattgt
     3541 gaactccttt aacagctcgc gctttggcat caactggcag ggttttacca ccaaatggta
     3601 tagcctgctg atgaacaacg acagcctgtt acaggcagcg cagcattcac taacaatggc
     3661 ggtgttttcg gcgacgtttg ctacgcttat cggttcactg acggcagttg cgctgtaccg
     3721 ttatcgcttt cgtggtaagc cgttcgttag cggaatgctg tttgtggtga tgatgtcacc
     3781 agatatcgtg atggcgattt cgctgctggt gctgtttatg ctgctgggta ttcagcttgg
     3841 cttctggtcg ctgctgttct cgcatatcac cttctgcctg ccatttgtgg tggtgacagt
     3901 gtattcgcgc ctgaaaggtt ttgacgtgcg aatgctggaa gcggcgaaag atctcggtgc
     3961 cagcgaattt accattctgc gaaaaatcat tctgcctctg gcaatgccag cggtagcggc
     4021 gggctgggtg ttaagcttta ccctgtcgat ggacgacgtg gtggtttctt cgtttgtcac
     4081 cgggccgagt tatgaaattc tgccgttaaa aatttattcg atggtcaaag tcggcgtatc
     4141 gccggaagtt aacgcgctgg caaccatatt actggtgctg tcgctggtga tggtaattgc
     4201 cagccagctt attgctcgtg ataaaacgaa aggtaacaca ggggacgtta aatgaaaaaa
     4261 tggtcacgcc acctgctcgc ggcgggtgct ctggcactgg gcatgagcgc cgctcacgcc
     4321 gatgacaaca acacgctgta tttctacaac tggaccgagt acgtgccgcc aggactgctt
     4381 gaacagttca ccaaagaaac cggtattaag gttatctatt cgacttacga gtcgaacgaa
     4441 accatgtacg cgaagctgaa aacgtacaaa gacggtgcct atgacctggt ggttccttca
     4501 acctattacg tcgataaaat gcgtaaagaa gggatgatcc agaagatcga caagtcgaag
     4561 ttaagcaatt tcagcaacct cgatccagac atgctcaaca agccgtttga cccgaataac
     4621 gactattcca ttccgtatat ctggggtgca acggcgattg gcgttaacgg tgatgcggtg
     4681 gatccgaaat ctgtcaccag ctgggccgat ctgtggaagc ctgagtacaa aggcagcctg
     4741 ctgctgaccg acgatgcccg tgaagtgttc cagatggcgc tgcgtaagct gggctactcc
     4801 ggtaacacca ccgatccgaa agagattgaa gctgcataca acgagctgaa aaaactgatg
     4861 ccaaacgtcg cggcgtttaa ctccgataac cccgctaacc cgtatatgga aggcgaagtt
     4921 aacctcggca tgatctggaa cggttctgct ttcgttgcac gccaggcggg tacgccaatt
     4981 gatgtggtat ggccgaaaga aggcggcatt ttctggatgg acagcctggc gatcccggca
     5041 aatgccaaaa acaaagaagg cgcgctgaaa ttgatcaact tcctgctgcg cccggatgtg
     5101 gcaaaacagg ttgctgaaac tatcggttat ccaacgccaa accttgcggc gcgtaagctg
     5161 ttaagtccgg aagtggcgaa cgataaaacg ctctacccgg atgctgaaac cattaaaaat
     5221 ggtgaatggc aaaatgacgt aggctccgcc agcagcattt atgaagagta ttatcagaag
     5281 ctgaaagcag gacgttaatt cagcaagtgg gcggtggtgg tattaccgcc cgttttttat
     5341 ttagcaagga tgtggctaat gagccaggac agtagagttt tctttcgtat attcttcggt
     5401 atcggtctgg tactgatcct catttctgtt gttatctttt ataaccagtt cacctatagc
     5461 aaagacgcga ttcatactga aggcgtcatt gttgataccg tctggcactc cagtcattct
     5521 catcgcacgg gtaaaaatgg ttcgtggtat cccgtcgttg cctttcgtcc gacgccagat
     5581 tacacgctaa ttttcaattc gagtattggc agcgattttt atgaagacag cgaaggcgat
     5641 aaagttaatg tttattattc tccaggacat cctgaaaagg ctgagataaa taacccgtgg
     5701 gttaattttt ttaaatgggg gtttatcggc attatgggcg ttatttttat cgccgtcggt
     5761 ttattaatca gtatgccctc ctcaaaaaag acacgcagga aacggaagtc ccgcccatga
     5821 aaaaaatcat aatattgctc agtttactga tactcctgcc tctaacggcg accagtaatc
     5881 cattgattcc aataatgaag actcttttta cagatgttac cggtactgta ccagatgctg
     5941 aagagatcgc gcataaagcc aagctttttc gccaacaaac cggagttgca ccgtttattg
     6001 tggttttacc cgacattaat aatgaagcca gtctcaggca aaatggcaaa gcgatgctgg
     6061 cgcatgcggc atcttcaatg agtaatgtaa aaggcagcgt cctgttactc tttactaccc
     6121 gcgagccacg gttcattatg ataaccaacg gccaggtcga aagcagtatg gacgataaac
     6181 atctcggcct tctggtagaa aatcacacgc tggcttattt acatgcggat ctctggtatc
     6241 agggtatcaa taatgcattg gctgttttac aagcacagat attaaaacaa ccgacgccac
     6301 cactgacata ttatccgcat cccggacaac agcatgaaaa tgatccaccc ggcagtacca
     6361 cgacattagg cctttttgcc tgggcagttg cattcatcgt ctttgctgca ttttttaact
     6421 acacaacgcg cctttattat gcgttaaaat ttgcagtggc gatggctgtt gccaatatgg
     6481 ggtatcaggc tttatgtttg tatatcgata acagctttgc gattaccaga atatcgccac
     6541 tctgggcagg gctgattggt gtctgtacgt ttattgccgc gttactatgg acgagtaaac
     6601 ggtaaaagag gcagtaaagt tcgcatgtat tatgccggat gcggcgcgaa cgccttatcc
     6661 ggcccacaaa acccgcaaat tcaattaatt gcgccccttc acaggcctga caagcgtagc
     6721 gcatcaggca atttttcccg ctttataatc ccttcagcaa cttttcaaca aactctggca
     6781 ccacctggct tgccggaccg taatatttct cggcaaattc attaccaacc tgactcggtt
     6841 caagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     6901 ccggataaac atgccccgaa gtaccaatgg caatgaaaat atcggccatc gacaacgcca
     6961 tataaatttc atccatgccg agtggcattt cgccaaacca cactacgtgc gggcgcaagg
     7021 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     7081 ggacctggcc actttgcgaa caacgtactt tcagcagttc cccatgcata tgaatgacat
     7141 tggtattacc tgcgcgttca tgcagattgt cgatattctg cgtcaccagc aaaaagcgat
     7201 cgccgagagc atcttgcagt ttagccagcg caagatgtgc agcgttaggc tgaatttctg
     7261 gctgctgcag ctgtcgacga cgggcgttat aaaacgtttg caccagttca ggatcgcgat
     7321 caaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     7381 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     7441 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     7501 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     7561 gcatagcaac ctctgttgat taatcggtta gatgtaggaa ggccgcacca cgcattcccc
     7621 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     7681 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag cctccaccaa
     7741 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     7801 ccagataacg ctcaacgtgc gcccttgcct gctcatcgtc ttgatcataa agcgcaatga
     7861 tctcaggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     7921 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     7981 ccagccccat catggttaac gcatcaaccg gcagacgcat atggccaaat tcgccggtaa
     8041 tatagctttt accggtaatt ggtttaccgt tgaaaatcag cccgccgcca acgccggtgc
     8101 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccat gcttcagaaa
     8161 gggcaaaaca gttggcatcg ttatcaaggc ggacatcgcg atcaagacgt gcgctcaggt
     8221 cggcacgcag cggtttaccg ctggcagcag ggacattggc ggcatacagc gtaccatctt
     8281 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgccggt
     8341 cagcttcggc taccagctcg cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     8401 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcct agcgcaattt
     8461 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     8521 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     8581 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     8641 aattccgatg gcaagaagtc aataaaatag atatcgctgg agaggaactg atgaccgatc
     8701 agcttttcaa tccactcaat aatcggggta agttgcagtg aaaccactac gccgataatc
     8761 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     8821 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     8881 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     8941 gccagataca taatggcgcg gatcatctga atatcgcgat acatatagcc gtaagtacca
     9001 atccagcttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     9061 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     9121 agatattgct gagcatcggc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     9181 aactgcaaaa taccggcaat gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     9241 ggattcgaat ttgggatcat aatcgacacc caatcgccct gcttcacttt cagcgcatcc
     9301 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccaggcatcc
     9361 ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     9421 acctggattg cgcgcagatt cgcgccgctc tccaccagcc cggtgaaatt gatatacggc
     9481 gcagcggcgg caatgcccgg cactttctgc acgttatcca gtgcttcctg ccagttggtc
     9541 cacggctgat ctaccgcctc aatttcaccg tgcggtacta ccgccagaat gcggttattc
     9601 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgcccagg
     9661 gcaatgccaa tggtagaaat gacggagatc aacgacacca tgccgccgcg tcgccgtccg
     9721 cggctaaaac gcaggccaat caataacgat aaaggcatcg ccattactcc gcccccatca
     9781 ggctcagttc cgccgtcaaa cgtccatcac gcatttccag ttggcggctc atacgtttcg
     9841 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     9901 caagcaactg gaagatgctg tcggcgttac gtgcatcgag gttaccagta ggttcatccg
     9961 ccagtaccag ccgcgggtta ttgaccagcg cacgggcaat cgccacacgc tggcgttcgc
    10021 cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca
    10081 tctccagcgc acggctgttg atttcagcgg gctttttctt gccaatcaac agcggcatag
    10141 ccacgttttc cagggcggta aaatccggca acaggtggtg aaactgataa ataaatccca
    10201 gcttctggtt acgcagttcc gctttggccg ccgacgacag tttgctcatc ggttgaccgt
    10261 taaagatcac atcgccggag gttggcgtat ccagcccgcc taacaggtgc agcaaggtgc
    10321 ttttaccgga accagagctg ccgacgatcg ccatcatttc accttcgccc acgctgaaac
    10381 tgacattgtg cagtacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
    10441 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
    10501 cgccaggaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
    10561 atgacctgta aaggctcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
    10621 atcggcatca gattattcaa ctggctggca agcagtgcgc caagcgccgc tccgaggatc
    10681 gcaccgataa tcccagcgct ggccccttgc accataaaga ccatcatgat ttgacgcgga
    10741 gtcagcccct gagtctgaag aatggccact tcgccctgct tctccatcac catcagtccc
    10801 agcgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag caagcccatc
    10861 atattttttt ccatgcgtac ggcctggaac agctcgcctt tacggtcacg ccagtcctgc
    10921 catttgctgc cttcaggcag tttttgctga cttaatgagt cgactttcag cggctcatcc
    10981 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
    11041 tcaatattca ccagcatttc atagccatcg acttcactgt tggcggcgaa agtgccaatc
    11101 acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggc
    11161 accatcacgc ggatttgatc accgcgattg acgcccaact gtgaggcaag ctgttcaccg
    11221 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc
    11281 gttagtggat ctttttgcgc cgggtcgata cccagcatca ccccgaccgc cacgctgcgc
    11341 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
    11401 ttgacggccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
    11461 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
    11521 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
    11581 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    11641 gcgacaggtt ggtacatgaa atccgtctgg ttgctgttag caaagacacg agtatataag
    11701 cgaaagcggg atgggtaaac gtctgaactg taagtgatcg tcagggctaa tctgaaaagt
    11761 accggcaaat caatcggcta aagtgcctca ttttatcatc ggccatgtgg cctgatcttt
    11821 atctgaaaaa taaccaaata cagacagata cgcattacac cacgcgactc gccccgcagg
    11881 atcaaccgca tgttaaataa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    11941 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    12001 tttatccgca tctgaccact ttccagcatc cgttatcaga agtcctgagc aaatgctgga
    12061 tctatttcaa tctttacctt gcaccctttc gtatgccggt ttttttcttt atctctggct
    12121 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacgcatct
    12181 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ctaagtgcac
    12241 taaatcagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    12301 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    12361 atgcgttaat tgtctatttc gtgatatgta aaatttttaa ccgcctggcc ctgccgttat
    12421 ttgttctgtt tgtactgatg agtgtggctg ttaatttcgt acccacaccg tggtggggaa
    12481 tgaacagtgt gatccgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacat
    12541 taatgacctg tgttaaagcg gtaccgttgc gccgccatct gctgatggct tctttgctga
    12601 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    12661 ttgtggtaat catgaagctg ttttatcagt acgagcagcg tttcggtatg cgttcctcca
    12721 gcctgctgaa tgtgattggc tccaacacca ttgctatcta taccacccat cgcattctgg
    12781 tagaaatatt cagcttaagc ctgattccgc aaatgaacgc ggtacgctgg tcgccgcaag
    12841 tcgaactgac gctcctgctg atttacccct ttgtcagttt gttgatttgt actgttgcgg
    12901 gcttgctggt aagaaaactt tcacagcgcg cattcagcga tctgttgttc tccccgcctt
    12961 ctctgcccgc tgccgtcagt tactcccgct gagccatcgc cccagccggg gcgattcaat
    13021 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    13081 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    13141 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgctgcctgc
    13201 gcgacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    13261 gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc agttcaccga tcagatggtg
    13321 atgaatctgg cggactggga aaccctccct tacgacagtt tttcgcctca tcaggacatt
    13381 atctcctcgc gtctttccac cctttaccag ctaccgacca tgcagcgtgg cgtactgatt
    13441 gttccggtga atacgcttat gcagcgcgtt tgcccgcaca gctttctcca cggtcatgcg
    13501 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    13561 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    13621 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    13681 gaaatcgaca gcctgcgggt gtttgacgtc gatagccagc gcacgctgga ggaagtagat
    13741 gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    13801 cgcagccagt ggcgtgatac cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    13861 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccactatt cttcagcgaa
    13921 ccgctaccgc cactgttcag ttatttccct gccaatacgc tgctggtaaa tactggcgat
    13981 ctggaaaaca gtgccgaacg tttccaggct gacacgctcg cgcgttttga gaatcgcggt
    14041 gtcgacccaa tgcgcccact gttgccacca caatcgctct ggctacgggt agacgagctc
    14101 ttttcagagc tgaaaaactg gccgcgtgtg cagctaaaaa ctgaacattt accgacaaaa
    14161 gcagcgaatg ccaatttagg tttccagaaa ctgccagacc tggccattca ggcacaacaa
    14221 aaagcgccgc tggatgcgct gcgtaagttc ctcgagtctt tcgacggtcc ggtggtgttc
    14281 tcggtagaaa gtgaaggccg ccgtgaagcg ttgggtgaac tgctcgcgcg aattaaaatt
    14341 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    14401 ggcgctgcag agcatggttt tgtcgatacg gtacgtaatc tggcgctgat ttgcgaaagc
    14461 gatctgctcg gtgaacgcgt tgcccgtcgt cgtcaggatt ctcgccgcgc catcaacccc
    14521 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    14581 cacggcgtcg gtcgttatgc cggaatgacc acgctcgaag ctggaggcat tactggcgag
    14641 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    14701 ctgattagcc gttacgcggg tggcgcagaa gaaaacgcac cactgcataa acttggtggc
    14761 gatgcctggt cacgcgcacg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    14821 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    14881 gagcagtatc agttattctg cgacagcttc ccgtttgaaa ccacgccgga tcaggctcaa
    14941 gccattaatg cggtgcttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    15001 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcggtagat
    15061 aaccacaaac aggtggcggt gctggtgcct accacccttc tcgcgcagca gcattacgac
    15121 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    15181 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    15241 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    15301 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg
    15361 aacgtggata ttctgacgct taccgcaacg ccgatcccac gtacgctgaa tatggcaatg
    15421 agcggaatgc gcgatctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    15481 acctttgtcc gtgagtatga cagtctggtg gtccgggagg cgatcctgcg tgaaattttg
    15541 cgcggaggac aggtttatta tctctacaat gatgtggaaa acatccagaa agccgccgaa
    15601 cggctggcag aactggtacc tgaagcgcgg attgccatcg gtcacgggca gatgcgcgag
    15661 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    15721 accaccatta tcgaaaccgg aatcgacatc ccgactgcca acaccattat cattgaacgc
    15781 gcggatcact tcggtctggc gcagctgcac cagttgcgcg gtcgcgtcgg acgctcgcat
    15841 catcaggcat atgcatggct gctgacgccg catccgaaag cgatgactac cgatgcgcaa
    15901 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggctttgc gctggcaacg
    15961 cacgatctgg agatccgcgg cgcgggtgaa ttgcttggcg aagaacagag cggctcgatg
    16021 gaaaccatcg gtttctcgtt gtatatggag ttgctggaaa acgccgtcga tgcgctgaaa
    16081 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagttgcgg
    16141 atgccgtcgc tattgccaga tgacttcatc cctgacgtga atacgcgttt gtcgttctat
    16201 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    16261 gatcgcttcg gtctgctgcc ggatccggcg cgtaccctat tggatattgc ccgtttgcgt
    16321 cagcaagcgc agaagctggg gatcaggaag ctggaaggta atgagaaagg cggcgtgatc
    16381 gaatttgccg agaagaatca cgtgaatccg gcctggttga ttggtttgct gcaaaaacag
    16441 ccgcagcatt accgccttga tggcccgacg cgcctgaagt ttattcagga tttgagcgag
    16501 cggaaaacgc gtatcgaatg gattcgccag tttatgcgtg aactggaaga gaacgcgatc
    16561 gcttgatggt aaatcggggg cgtctctgcg cccccatctg caacatttac aaattctttg
    16621 cacttccctg cactatccga cactgacacc atccataatt cagacttatc tttttattac
    16681 aatagcctta tatttattat ggactttggt gatgatcaaa acgcgttttt ctcgctggct
    16741 aacgtttttt acgttcgccg ctgccgtggc gctggcgcta ccggcaaaag ccaacacctg
    16801 gccgctgccg tcagcgggca gtcgtctggt tggtgaaaac aaatttcatg tggtggaaaa
    16861 tgacggtggt tctctggaag cgatcgccaa aaaatataac gtcggctttc tcgctctgtt
    16921 acaggctaac cccggcgttg atccttacgt accgcgcgcg ggtagcgtgt taacgatccc
    16981 gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt gtgatcaaca ttgcggagct
    17041 gcgtctctat tattatccgc cgggaaaaaa ttcggtaacc gtatatccca tcggcattgg
    17101 tcagttaggt ggtgacacgc tgacaccgac aatggtgacc accgtttctg acaagcgtgc
    17161 aaacccaacc tggacgccaa ccgcaaacat ccgcgcccgt tataaggcac agggaattga
    17221 gttgcctgcg gtagttccgg ctggaccgga taacccaatg ggccatcatg cgattcgtct
    17281 ggcggcctat ggcggcgttt atttgcttca cggtacgaac gctgatttcg gcattggcat
    17341 gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat atcaaaacac tctttagcca
    17401 ggtcacccca ggcaccaaag tgaatatcat caacaccccg attaaagtct ctgccgaacc
    17461 aaacggtgcg cgtctggttg aagtacatca gccgctgtca gagaagattg atgacgatcc
    17521 gcagctgctg ccaattacgc tgaatagcgc aatgcaatca tttaaagatg cagcacaaac
    17581 tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc gggatgccgg tggatgtccg
    17641 ccgtcatcaa gtgagcccac aaactctgta aagctcggat aaaaaaatcc ccgcgacacg
    17701 gcatgtcgcg gggaaaaaac aagcagtagc aggcattaat gagggttaat gcttatttat
    17761 aaattactgc tgttccatgg agggtattcg gaccggttac agaagtaata cggaaagatt
    17821 ttgcgcccat ctcatccgct ttttgcgcca gctgctcttc cagcgatccc agatttgtcc
    17881 ccgcgttagc actgatagta ccgacttttt gttggccttc tggcgttgac tgaacttcga
    17941 cagccgcaaa gctggcaaat gacatggagc ttaaaatcgc cgcagcgatg agggttttta
    18001 cgtttttcat aatagtggcc ttatgcagat gaatgacgtc gcaagcaatt acttaacgat
    18061 cgataactaa atgatagatg tgatctggat cacatacaag atatttttta taacaatcat
    18121 taattaattt aaaaaccttt aaatttcagt taaatagaaa gcgattattt ttatggtctc
    18181 tggactggtg aacggaggtg attattttta taataacctt tcattcacag tcaccaggta
    18241 caacggcatc atggcaactg actcaacaca atgtgtaaaa aaaagccgtg gccgcccaaa
    18301 agtgttcgac agggatgccg cgcttgataa ggccatgaaa ttgttctggc agcacggtta
    18361 tgaagcgact tctcttgcgg acctcgttga agcgaccgga gctaaagcgc ccacgctata
    18421 cgcggaattt accaacaaag aggggttatt ccgcgccgta ctcgaccgct atatcgatcg
    18481 ttttgccgct aagcatgaag cacagctgtt ttgtgaagag aaaagcgtgg agtctgcgct
    18541 ggctgactat tttgctgcca tcgccaactg ctttaccagc aaagacactc cggctggctg
    18601 cttcatgatc aacaactgca ccaccctctc cccggattca ggagatatcg ccaatacgct
    18661 gaaatcacgc catgcgatgc aagagcgcac tttgcagcag tttttatgtc aacgacaagc
    18721 gcgcggggaa atcccgaccc actgtgacgt gacgcatctg gcagaattcc ttaattgtat
    18781 tattcagggg atgtcgatca gcgcacgcga aggtgcatcg ctggaaaaac tgatgcagat
    18841 tgcccgaacg actttgcgtt tatggccgga actgctgaaa taggtcgcaa aaaaagcccc
    18901 tcagcttgag cgaatgaggg gctaaattgc agagtacaac cttgttatgc tttgttattc
    18961 aaaatcagct gcccgttgct atctagcggg atctgcgtac ccggatcgcg gtccattcgg
    19021 attttgccct gctgatcgcc aatcttatag gtcacatcat aaccgagcat tttttctgac
    19081 ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt aagtatcgct ttcctggaga
    19141 gagccctgga tctggttacc tgcatatcca ccccccagcg cccccacaac agtggcgaca
    19201 tctttaccgc gaccaccacc aaactgatgc ccaatcacgc cgccagcaac agcgccgagt
    19261 accgacccgg taatgcgatt ttcatcctgc accggtcgac gatgggtcac tgtaacgttg
    19321 cgacactcct gacgcggtgt tttaaccgtt tccttgattg gggttgcaga aacaacctga
    19381 gcgtattgcg ggccacgttc aaacacgttc agactggcca ctgccgctac gcccagcgca
    19441 gctgcgacac caatcccgat acccgccaac attgacttat tcacgggaat acctccttct
    19501 ttgctgttat gccaatcatg caagcgaagg agaaaagcag ctatcagaca gaagatgaaa
    19561 agcgcgggaa tggctggaaa tacttaggga ttcggaggag tctgaaggaa tgcctgatgc
    19621 gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat ttgtccaggg cggataaggc
    19681 gtttacgccg catccgccag tacaagccga ttaatgcaac ttcaaacgtg gacggataac
    19741 gcggttaata ctccccacca gcatcattaa tccggtttta aagtaaccat gcagcgcaat
    19801 ctggtgcatt cggtacagcg agatgtatac aaagcgcgca attcgtcctt caatcatcat
    19861 tgagccgcgc gtcaggttac ccatcaggct accgactgtg gaaaagttcg acagcgatac
    19921 cagcgaaccg tgatctttat actgataatt tttcagcggc ttaccgttca tctgcgccag
    19981 aatgttgttc attgcacagg ttgccatctg atgtgcggcc tgagcgcgcg gcggaacaaa
    20041 gcccccttcc ggacgcgggc atgacgcgca gtcgccaata gcgtatatgt ctggatcgcg
    20101 agtggtttgc agcgtcggtt ccaccaccag ctggttgata cggttggttt caagaccacc
    20161 gatatctttc aggaagtctg gcgctttgat cccggctgcc cacaccatca gatcagcctc
    20221 aatatattcg ccatctttag tgtgcaggcc gccttcatca gcactggtga ccatggtttg
    20281 cgtcagcacg cgaacgccaa gtttcgttaa ctcactatgg gccgcagcag agatacgtgg
    20341 cggcaatgca ggcaaaatac gttcgcccgc ttctaccagc gttacgttca gggcttcgtt
    20401 ggtcagacct ttataaccgt aactgtgcag ttgcttgacc gcgttgtgca attcagcgga
    20461 gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg tttactttgc cattcgcgcc
    20521 caggttggcg gagtatttca ggaacagatt cagcatctcc tggtggaagc gacgcgcctg
    20581 gtgcgggtta tcgaggaaaa tgcagttctc tttgacacct ggtgtattga aatcgttaga
    20641 ggtgctaccc agcgccatta ccagggtgtc ataggcgatt ttacgttccg gaaccagcag
    20701 ttcacctttc tcgtcgcgca gttctgcaat agtgattgtt ttcgcttcac gatcaatatc
    20761 aattacggaa cccagctgga actggaaacc atgattgcgc gcatgggcca gatagctcaa
    20821 cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg tgcagcagcg gtttccacag
    20881 gtggctgtgg ttacgatcga ccagcgtaat tttggctttt ttcttgcgtc ccagcttatg
    20941 ccccagctgt gttgccattt ccagcccacc agcaccgccg ccgacaatca caattttttt
    21001 caatggcgta gtcaacgtga cccccttaaa tttattaacc aattgttaat taatggttat
    21061 taacatagct cttaattaac aacaggttac agcaatgaaa atgttcttcg ctattgagaa
    21121 taacatgaat ggtgcattgg tcataccaaa attgatgtta atcaagtttt gttgctgaaa
    21181 agataggcga ctttaccggg tgagatcaaa aaacagagtt tgttgtttat tatttgagct
    21241 aatttgtgag aatgatatag agtgaggtgt gtatggaggg cgttcggtag gcctgataag
    21301 aggcgcatca ggcattgatg ctcgactgtc ggatgcggcg gaacgcctta tccaacccac
    21361 agaatccgga gcgcatcagg taccggcaag gttacctgat gcgacgaggg catttacccg
    21421 agggttttga acgctttaat gcgctgtaaa tgcggggaga tattcttgaa tttgtgcgtc
    21481 tgctcttcgt cccagacaat ctcgtaataa tgatgcaact cttcagatgt ccgctggctg
    21541 ttaagcgctt catcattacg cgacaaaatc accaggcaac gatcgcgatt cttctcacgg
    21601 aagttggtca cacacttagt ggcaatatcg gcatactctt ccgggcgatc aatcttccct
    21661 tccatgttct cataagggaa caaattgggg ttgaagatca cctggcggat atcacagaga
    21721 aaaccaatcc gctcagccca ataaccgccc agaccaacac cacaaatgag cgggcgctcg
    21781 tcgacgttca gttgcaacat tttgtccact tctttgagca aatgctgcat atcatgtttc
    21841 ggatgccgcg tactgtagct tattagccgt acatccgggt caataaactg caattgtaag
    21901 actttctcgt ggttacccgg actgttagag tcaaaaccgt gcaaatagat aatcatcgct
    21961 tcctcacata agccagggtt aatgacctgc cttctcttcc tgccagcgtt catgtaactg
    22021 attcagacgg gcgctgatcg ctttccagcg agccgagtcc atcagttcct gtcgcgaaaa
    22081 tgaaccttta tgatacaaac gtgtaacacg ttctgccttg atcggtgaca gattatctaa
    22141 cacgctgacg gcccctttac gattattgca gaccaggatc atatcgcaac ctgcatccag
    22201 tgatgcctgt ccacgctcgg cataactgcc cataatcgct gcgccttcca tcgataaatc
    22261 gtcagagaaa atcacgccgt caaaacccaa ttcctgacgc aaaacggttt tcagccagta
    22321 ggtagaaccg ctcgccggac gcggatcaac atcactgtag atcacatgcg caggcataat
    22381 ggcgtcgagt ttattttcgc ggattaagga actgaagacc gacatatctt tagcgcgaat
    22441 ctccgcttgc ggacgtgggt cgcacggtgt ttctttgtgt gagtctgccg ttactgcacc
    22501 gtgacctggg aagtgtttcc cggtagtttt cattcccgct tcatgcatac catcaataaa
    22561 acggctggca attgccagag ctttttgtgg atcggcatga taagaacgct cgccaatcgc
    22621 cgcgctgata tgcccgacgt ccagcactgg cgcaaagctg atatcaatat ccatagcaat
    22681 catttcgctg gccatcaacc aacctgcctc ttgcgccagt tttccgcctt cttccattcc
    22741 caacagcgca gcgaatgatt gtgccgctgg caagcgggta aaaccttcac gaaaacgctg
    22801 cacgcgtcca ccttcctgat ctaccgccac caccagatga ttgcgcgatg ctgcgcggat
    22861 ctggcgcacc agttcacgta actgggcagg atcatgatag tttcgcgtaa agagaatcag
    22921 ccctcccacc agcggatgcg ccagtatttc acgctcttcc gcgtccagtt cgtaaccttc
    22981 gacatccaac attactggac ccacactgct ctccttattg ttttattaat agctgccgcc
    23041 aggtgtcatc ggccagcctg ataaattgtt gatcgccggt ttgtcgccag cggtactcaa
    23101 accaccctgc tttgagcatc agcagccagg gaaaccatcg cctgacctga cgccataatt
    23161 gcgccggata aatcttcgcg cgagtggcat agtcattgac caattgccga tgctggtcag
    23221 tattttccac ccacaccgcc gccagttcca gcgcgatatc accatctccg gcatactccc
    23281 agtcgatgag ttttaaccct gacgcgctat gcactaaatt tccggcgtgg acatccatat
    23341 gcaatggact taagcgtaaa agccgtggtt cccgcgcttt acgcagacgt tttaacattc
    23401 gcagccaacc cactgtccgc cgcgccggat cgctttgctg ccagtacagt tccagtaacg
    23461 gcaacagcgt tattcgccag ccaaaacgcg gttgttgatg tagataatac agcaagcctg
    23521 ccagttcgtt ggtatccggc aaatacgttt ttacctcgcc gggcagatag tcaaccacca
    23581 tccagtcacg gagatataaa tgcggcttcg gtgcaatgca tgcgggtagt tgtgataaag
    23641 cccgatactg gcgcaagaac gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa
    23701 caaaacgctg gccctgatgt tcaatgagaa aactcccgcc actaagccct cgattaaacg
    23761 tcgtgacggg atgaaactgc gggaaaaagc gcgacagcaa ttcgtcgcgc gtgaggggat
    23821 tattgctgcg aaacggcacc tttacctgac cagataattt cgcccgtctg caccagcatc
    23881 agctgcattt gtagtgtcgg agcgttaacg ttgccagagg cgctggagta cagcacgtaa
    23941 tgagcgccaa cattgcgggc aatgcctatg gctttactac gggtgcctaa actgtcctgc
    24001 ggcgacaaac ctaattgttg cttagccatc gacagctgct gggcggaaac cagggtaaat
    24061 ttaccgttat tagccagcgc atttcgcagc gtttcggtcg cttctgcggc attcaacgaa
    24121 ccgttagtac ggttgttaac gctatcaacc agcaggacgc tacccgcagt cacgccgtca
    24181 gccccaagca tcttactgac catcggctgc attgcgccat tccagtcata atggcgaata
    24241 tgcggcgcag gcggtgcagt ttgatcttcg tgctcaattg ggcctggctg ctgcggtatc
    24301 gtcggcaccg atggcactgt ggggacaggc tgttgtggct cagcaggttg ttccggcgct
    24361 ggtttcactt cttctaccgg tgcaggttca cgttgcccca cacacccggc aagaaacatc
    24421 gccagcgcgg taatcaaggc gtagcgactc atttttgtca tcaagattca ccccttacaa
    24481 atatagataa agtctgactt tgtgcgcccc cagaaaattg gcgctgccgt acagcgtgac
    24541 cgccgaatgt gcgggaatgg taacgctgcg tggcctttcc agaggatgca tctccagccc
    24601 tctggcgtca taccagtaaa aacgataatg aacggtaacg ggttcttgcc tttcgttata
    24661 aagcgttgag gatgctgaag gttgaatatc agatgtcgaa aggacgggct tttctgcact
    24721 gatgcccgca gctaataaag atgactccat caccagcgat tgctcatcat tcaccggaat
    24781 ttccggatgt gaacgacagc ccaccagcac taacaacgcc agagacatca gcccaaagca
    24841 tccttttctc atcgttacag acctttatgc gccagcattg gtcccagcgg acagccaccc
    24901 aacaaatgca tatggatgtg gtaaacctct tgtccgccat ggcggttggt gttcatgatc
    24961 agacgatagc catcttcggc aataccttct tgctcagcaa tttttgccgc tacggtgatc
    25021 atgcgtccca gcgcctgctc atgctcagct gacacgtcgt tcacagtcgg aatgaggata
    25081 ttcggaatga tcagaatatg cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc
    25141 agatcatcct ggtagacgat atcggagggg atctcacgac gaataatttt gctgaatata
    25201 gtttcttctg ccacgacatt ttcctttttc aaatagccat gcgttgcgcc atgctacgcc
    25261 gcgccacact gcgagtatag agtatggtcg agttaaacct gctctttcaa ccttaactca
    25321 cgatttctta agcaaaaaat cgttttgctg ccgtatatat cgccattatt cccatttctg
    25381 cctgcgaatg cgctgaaaag acattctctg tttggtttac actccaaata tgaatgcgta
    25441 tatttctcat ttgcatttac acacaaaaat tattcgcaca ctaaatataa ccgctcatca
    25501 ccctgcttcg gcatgagcct gacgccaaca taaaccaaga gatttcaaat gctttcgaca
    25561 caatttaaca gggataatca acatcaagcc atcatcaaac cgtcactcct tgccggttgc
    25621 atagcactgg cactattacc ttccgccgct tttgctgcac cagccactga agaaacggtg
    25681 attgttgagg gttcagccac agccccagat gatggcgaaa atgattacaa cgtaacgtct
    25741 acctctgcgg gtacaaaaat gcagatgact caacgtgata ttcctcagtc ggtcactatt
    25801 gttagccagc agcggatgga agatcagcag ttacaaacgc tgggcgaagt gatggaaaac
    25861 acgctgggga tcagcaaaag tcaggcggat tccgatcgtg ctctttatta ttcccgcgga
    25921 ttccagatcg ataactatat ggttgatggt atccccacct attttgaatc gcgctggaat
    25981 ctgggcgacg cactttctga tatggcactg tttgaacgcg tagaagtagt gcgtggcgcg
    26041 acaggcctca tgaccgggac gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac
    26101 gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat acggtagctg gaacaaagaa
    26161 cggtatgtgg cggatttaca aagcccactc accgaagacg gtaaaatccg cgcacgaatt
    26221 gtcggcggct accagaataa cgactcatgg ctggaccgct acaacagtga aaagaccttc
    26281 ttctccggca ttgtcgatgc tgatttaggc gatcttacga tgctttcagc cggttacgaa
    26341 tatcagcgta ttgatgttaa tagcccaacc tggggcggtt taccgcgctg gaatactgat
    26401 ggcagcagca acagttacga tcgcgcacgc agtaccgcac ctgactgggc gtacaacgat
    26461 aaagagatca acaaggtctt tatgaccctg aagcagcggt ttgctgatac ctggcaagcg
    26521 acgctgaatg ccacccactc tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc
    26581 tatgtaaaca aagcggatgg tatgctggtt ggaccataca gtaattatgg acctggtttt
    26641 gattatgtcg gcggaaccgg ttggaacagc ggcaaacgta aagttgatgc gctggatctg
    26701 ttcgctgacg gtagttatga attgtttggt cgtcagcaca atttaatgtt tggtggcagt
    26761 tacagcaaac aaaacaatcg ttacttcagt tcatgggcca acattttccc ggatgaaatt
    26821 ggcagtttct ataactttaa tggcaatttc ccacaaaccg actggtcacc acagagcctg
    26881 gcgcaggacg ataccacaca tatgaaatcg ttatatgctg ccactcgtgt cacccttgcc
    26941 gatccgctgc atctgatcct cggcgcacgt tataccaact ggcgggttga tacgctgact
    27001 tacagcatgg agaaaaacca caccacgcct tatgctggac tggtgtttga catcaatgac
    27061 aactggtcga cctacgccag ctatacctct attttccagc cgcaaaatga tcgtgacagt
    27121 tcaggcaaat atctgactcc aatcaccggt aacaactacg agctgggtct gaaatcggac
    27181 tggatgaata gccgtctgac caccacgtta gccatcttcc gtattgagca ggataatgtc
    27241 gctcagtcca ccggtacacc tatccccggc agcaacggcg aaaccgccta taaagcggtg
    27301 gatgggacag tcagtaaagg tgtggaattt gaactcaacg gcgcaattac cgacaactgg
    27361 cagctgacgt ttggcgcaac gcgctatatt gccgaggata acgaaggaaa cgccgttaat
    27421 cctaatctgc cacgcaccac ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca
    27481 gagttgacgg tcggcggtgg tgttaactgg caaaatcgcg tgtataccga caccgtgacg
    27541 ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc tggttgatct cttcacccgc
    27601 taccaggtga cgaaaaactt ctcgttacag gggaatgtca ataacctgtt cgacaaaacc
    27661 tacgatacca acgaggaagg ttctatcgtc tacggcgcac cgcgtaattt cagcattacc
    27721 ggcacgtatc aattctgaat aacacctgta aaaaaggcag ccatctggct gccttagtct
    27781 ccccaacgtc ttacggatta gtggttacgg atgtactcat ccatctcggt tttcaggtta
    27841 tcggatttag taccgaaaat cgcctgaaca ccagaaccag caaccactac gcccgctgcg
    27901 cccagtttct tcaggccagc ctgatccact ttagacacat cagcaacgct gacgcgcaga
    27961 cgggtaatac atgcgtcgag gttagtaatg ttttctttac caccaaatgc agcaaccaga
    28021 gccggtgcca tttcgctggt acctgtcgct tttgcgtctt cagtcgcgtc ttcacgaccc
    28081 ggcgttttca gatccagtgc tttaatcagc acgcggaaga tggtgtagta aacaatcgca
    28141 taaccgatac cgacgatcgg gaacagccac agtttgctgc tgttaccaga cagaacgatg
    28201 aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca tccccaaaag aatacagatt
    28261 gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg
    28321 aaggagaact cgatcggctc ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc
    28381 ataataccgc ccactttcgc gcggttttct ggtttagcag agtgccagat agcaattgcg
    28441 gcagctggca gaccgtacat tttgaacagg aagccaccag acagtttacc cgcagtcggg
    28501 tcacccgcca tataacgcgg aatgtcgccg tggaaaacct gacctgctgc gttggtgtat
    28561 tcaccaattt gcatctggaa aggtacgttc cagatgtggt gcagaccaaa cggtaccagg
    28621 caacgttcga tgaaaccgta aatgccaaac gcaactaccg ggttctggta agcagcccac
    28681 tgagagaagg tctggattgc agaaccaatc ggcggccaaa tgaaggacag cacaacgcca
    28741 gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc gtttaccggc aaagaagcca
    28801 agatactcag gcagcttaat acggtagaaa cggttaaaca tatacgctgc aatcgcaccg
    28861 gagataatcc ccccgagtac gccagtatcc gccaggtgtt tagaggcgat ttcttcagca
    28921 ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt taaccatgat gccataggca
    28981 acaactgcgg ccagcgcgga tacgccatcg ttattggtaa agccgagggc gacaccgatc
    29041 gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg cttctgccat aacatgcgat
    29101 acaacggcgg gcagccagct gaaattcgcg gaaccgacgc ccagcagaat acctgcgata
    29161 ggcagtacgg ataccggcag catcagcgat ttaccgacct tttgcaggtt agcaaatgca
    29221 ttcttaaaca taattgagag tgctcctgag tatgggtgct tttttacgtt ctcacgcgtg
    29281 gcaagggggg agagcctcgc cgtgtacagg gcatctaagc gccctttatt tattacacag
    29341 agtaaaataa ttcagtgccg atttgtttga cggctatcac gtttcaactt cagatcattg
    29401 gagaagttgc acattttgac aaaacatatg tcacaatatt tcaaacgtgg ttattcaccg
    29461 cccaattttc ggcggtgaac tttactcgtt ttagccatta attacgagct ttaaaaaaac
    29521 tcattcaacg gatggattga aggcgggaag cgtcgatgtg aaacagacgg gcgaagttat
    29581 cggtggttac ctgcgccagt tcttcaacgg caacaccttt caacacagcc atgtattctg
    29641 caacgtcacg aaccatcgca ggttgattct cttttcctcg atgcggtacc ggcgcaaggt
    29701 aaggtgagtc agtttccacc agtaaccgat ccagggggac atatcgcgca gcatcgcgca
    29761 gttgctccgc attacggaag gtcacaatgc cggaaaagga gatgtaaaat ccgagatcca
    29821 gtaatttacc cgccgtttct ctgtcctctg taaaacagtg tagtacgcca ccgcaatccg
    29881 tcactttttc ttcgcgcaga attgccagcg tatcggcgcg ggcgtcacgg gtatggacga
    29941 taaccggctt gttcagttca cgaccaatct ggatatgatg aatgaaggac tcttgctgac
    30001 gtactttagt ttccggtgtg taataataat ccagcccggt ttcacccagc gctacaacac
    30061 cctcttctgc cgccagacgg cgtaaatctt ctacatcgta gggatcattc tggttgagtg
    30121 gatgcacgcc acaagaaaat acgacgttgt cgcgttcgcc taccagatcc cgcatgtgta
    30181 aataacccgg taatgttgtg gcgactgcca gacaaaattt cacatcgcgt gcggcggctt
    30241 tcgccagaac gtcatccacg tccttatgca aagattcata atccagacca tcgagatggc
    30301 agtgtgagtc gactaaaaac atgatgtctc tcttaaagat gaggaaccgg tagcacaacg
    30361 cccggttgca ggtaatgctc aatacgcagt aaaagatcgg tgatgagaag ctcgcggttg
    30421 atgcctgtga cagacattaa ctgttcacga atgtggcaaa catcccccag tatagcctgc
    30481 aggcgcgagg gagaaagatg gtttgccagt tcgacgacca ggcccggcac atcaacattg
    30541 gtcacctgcg cagcgccatg atggcgtttt agcgcatcca tcagcaacgt tgccagccag
    30601 tgtaaacgtg ccggagcttg ttcatgatta agggccgcta gcagcgaata ccagtcgccc
    30661 gattgcacgc tatatgccag cgcctgacac aatgtttcac gagcctgcca gttatctccc
    30721 tgaaacaacg ccagtgccgc cccaggcgaa ccggcgctta agcgcaatgc ggcaagtagt
    30781 gcagcctgtg acattgtcac ttcgcgtgaa agccaggtca cggcgtactg ttccggcggc
    30841 ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg ccagtaaacg ttctggctcg
    30901 cgggtagcaa ggaaaaacca cgtctctgct ggtggctctt caagcgtttt cagcaatgcg
    30961 ttagccgcgg cgtcggttag taaggcagca tcggttaccc agacaacttt cgcaccacct
    31021 aagcgtgcgt gctcattcag cttttcggtg acctcacgta ccgcatcaat gcccagcgca
    31081 tttttccctt tttcgggagc cagggtgtag taatcgggat gtgtgccagc ctgcatcaac
    31141 tgacatccac gacagtgacc gcaacttttg tggccctgcg gttgttggca gagtaaataa
    31201 cggctcaggg cgtagattaa agcatcatcg cccatgcccg gtaacgcctg aatgagtagc
    31261 gcatggtgac ctcttctggc ctgatagctg gctaccagtt tttcgaaatc aggtcgtaac
    31321 catggatacc atctcatgcg tccaactcct tcacccagtt ggtcacggta gtgcggattg
    31381 catccatcac ggcctccagc ggctgggtgg catcaatggt atgaatgctt ttatcttgtg
    31441 ctgccagttc cagatagcgg gcgcgggtgc gattaaagaa atcgaatgac tcttgctcaa
    31501 tacgatccag ctcaccacgg gcgcgagcac gttttaagcc gacctctggg gtgacatcaa
    31561 gataaagcgt taagtcaggg cgaaaatccc cgagaacagc atcacgcagt gttgccagca
    31621 tatgttggtc aatacctcgt cctccaccct gataggcttg agtagagaga tcgtggcgat
    31681 cgccaatcac ccaggtgccg ttagccagcg caggtttgat gaccgtttct accagttgaa
    31741 cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt aatgacttca tcgcctaccg
    31801 atttgatatc cagcaccagg cttcttaact tttcggcaag ttgcgtaccg ccaggttccc
    31861 gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt ctcaaccacc acgttacgcg
    31921 cggtagtttt gcctgcgcct tccagcccct caatgacgat atacttactg cgcatttttt
    31981 tccttaagca ctttcagata atcctgcaca gacttgttat gactggcaag attggtatta
    32041 aacgtgtgac cacctttacc atcggccaca aaatagagat acggcgtttt tgccggatgc
    32101 gcagcagcct tcagcgaatc cgcccccggc gtcgctatcg cacctggcgg cagaccggta
    32161 atggtatagg tgttatacgc tgtcggcgtt tccaggtctg cacgagaaag tttgccatta
    32221 taacgctctc ccatcccgta aatcacggtc gggtcggtct gcaggcgcat accaatgcgt
    32281 aaacggttga taaatactga ggcaacctga tcgcgttcac tggcaacggc ggtttctttt
    32341 tcgataattg atgccatcgt caccaactgg tttttatctt tataaggcag accgtccgca
    32401 cgcccttccc aggcgctatc gaccgctttc accattttct tgtgcgctcg cttgagtaac
    32461 gcgacatcgg tggtattggc ggtatacatc caggtgtctg gccagaacca accttcaatc
    32521 cactccgggt tttccagttc aagtgcctgc gctacggtgg cgtacttatc gtcgctcagc
    32581 gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt agtcgctcag acgcatccct
    32641 tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt ccaacaattt cagcatctcg
    32701 cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt taaagtgaga aagatccggt
    32761 tcgatacgca gcaaccattg aaaaacccgt gggcgattga tgatcttatc ggcataaagc
    32821 tgttcaccta gcgccagacg tccggtccct ggcttaaggg taaatatcgt ctcttctttg
    32881 ataagcaatt tgctgtcggc aagatggcga accttccaga cgcccacacc agcggcgata
    32941 cccagtacca ccagcaataa caagattatc aataacactt ttttcatgac taattcgggc
    33001 gctcacaaag tggggctaaa tattcatata acgttgctga cgaaaaggag acatcgccac
    33061 aggcacgtac gggcatcact ggcattaacg cattacaaat aaccatctca tctgcctgca
    33121 acgcctcttc cagagaggct tgcacttcga caagctgata agaggattgt gccagcaaac
    33181 ggatacagaa ttgtcgcata atgccgttaa cacctgcctg atccagtcgc ggcgtataaa
    33241 ctacgttgcc cttccgccag aacaaattag ccgcacagca ttccgtaacc cacccttcgc
    33301 tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag atgagagcgg atcaatactt
    33361 gctcaagccg attaagatgt ttaatacctg caagatgagg attgcgcccc agccgcaccg
    33421 ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc gtaatgtgca ggataagccg
    33481 taacggagag aatccgcgtt gccggtccac tgttcaatgt gctgtaccct cgcccgccac
    33541 taccgcgact gatcacgact ttcagtacac cattttgctg ttctgctgcc agcgttttca
    33601 tctcttgttc aagctgaggc cagaagtcac aggaaatcat caaccgctga caagcatcct
    33661 gtagtcgctg gatatgcgcc gataacaaac tgactttacc gtcgataact ctggcggtgg
    33721 taaaacaacc atcaccaaac tgcgttgccc gatcgcttac tgccagcgat tcctgcttat
    33781 gaccgttaat taagaacata ccggctcctt atcagagaat tgttagtgtg gcagcatgtt
    33841 cactacggaa caagtcggaa taaaagctaa gaaaaaaggc ccgcaagcgg accttttata
    33901 agggtggaaa atgacaactt agatcttttt aaagatcaaa gaaccattag tgccaccgaa
    33961 gccgaaggag ttacacagag tgtattccat tccgctaacc tgacgcgctt cgtgcggtac
    34021 gaaatccaga tcgcaacctt catccgggtt atccaggttg atggttggcg gaacagcctg
    34081 atcgcgcagc gccaggatgg agtagataga ttctactgca cccgccgcac ctaacaggtg
    34141 accggtcata gatttcgtgg agcttaccaa cacgcggctt gcagcttcac cgaagatggt
    34201 tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta gaagtaccgt gcgcgttaac
    34261 gtagccaatc tgactcgctt caatgcctgc atcacgcaga gcatttgcca tcgccagagc
    34321 tgcgcctgcg ccattttctg gcggtgacgt catatgataa gcatcgctgc tcataccaaa
    34381 gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc gcgtgttcgt actcttcaag
    34441 taccagcata ccggcaccat cgcccagtac gaaaccatca cgctctttat cccacgggcg
    34501 gctcgccgct tgcgggttat cattgcgggt agataatgca cgtgccgcgc caaaaccacc
    34561 aacgcccagc ggcgtactgg ctttctctgc gccacctgca accatcacgt cagcatcgcc
    34621 atacgcgata atacgcgcag catggccaat gttgtgcacg ccggaagtac aggcggtcgc
    34681 gatagagatg cttgggccac gcaggccata catgatagtc agatgacctg ccaccatgtt
    34741 cacaatcgtt gacggaacga agaatgggct gatcttacgt ggaccaccgt tcatcagaga
    34801 tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg gagccaattg cggcaccaat
    34861 gcgggttgcg ttctcttccg ttatttcaag gccagaatcc tgcatggcct gaacgccagc
    34921 gacaattcca tattgaatga aggcatccat cttgcgctgt tctttgcgcg agataatgtc
    34981 ctcacagtta aaatccttta ctaagccagc aaatttcgtt gcataggcgc tagtatcgaa
    35041 atggtcgatt aggctgatgc cactctgacc ggcaagcaga gctttccagg tagactctac
    35101 ggtattgccg acaggagaca acatgcccag tccggtcaca actacacgac gcttagacac
    35161 gtttgtcctc cagggaggga aaaaatgatt ctagtgggac aaaagataaa actcaggcgg
    35221 tcgaacgacc gcctggagat gttcacttac gcctggtggc cgttgatgta atcaatggca
    35281 gcctgaacgg tggtgatttt ctcagcttct tcgtccggaa tctcagtatc aaactcttct
    35341 tccagagcca ttaccagctc aacggtgtca agagaatccg cgcccaggtc ttcaacgaaa
    35401 gaagcattgt tggtaacttc ttcctgctta acgcccagct gttcgccgat aattttctta
    35461 acgcgttctt cgatagtgct catactctta aatttcctat caaaactcgc tttcgcgatg
    35521 gttttcgtag tgtataaaat gttgaaaaat ttgcaactaa atcccggcag gtcttaccac
    35581 gattttacgt tattttgagg ccttaccccc caataacgca aatcattttg cactaattat
    35641 tactcataac cacgcagact gcgcgcaaca tgagcatttt gtgcaaatcg cggtcagacc
    35701 atgtacatcc cgccgttcac atgcaaagtt tcacccgtga tgtaagctgc ttcgtcggat
    35761 gccaggaatg caaccgcgtt ggcgatttcc tgtgcgccgc cgaggcgacc cgcaggaacc
    35821 tgcgccagga tacccgcacg ctggtcatcg ctcagcgcac gtgtcatgtc cgtttcaata
    35881 aagcccggag caacaacgtt tacagtaata ccgcgtgacg caacttcgcg cgccagtgat
    35941 ttactgaagc cgatcaagcc cgctttcgcc gcagcgtagt tggcctgacc gccatttccc
    36001 atggtaccaa ccacagaacc gatagtgata atacgaccat gacgcttttt catcatagcg
    36061 cgcattaccg cttttgacag acggaaaaca gatgaaaggt tggtttcgat aatatcgttc
    36121 cactcttcat ctttcattcg cattaacagg ttatcacgag tgataccggc attattgacc
    36181 aggatatcca cttcaccaaa ttctgcgcga attttttcca gaacagattc gatagatgcc
    36241 gggtcggtca cattcaacat cagacctttg ccgttggcac ctaaataatc actgatcgcc
    36301 tgagcgccat tttcactggt cgcagtgcca ataactttcg cgccacgggc tgcgagcgtt
    36361 tcagcaattg cgcggccaat tccgcggctt gcaccggtta ccagtgcgat ttttccttca
    36421 aaattcatga ttttcctctt ttaaagctcg agcgccgctg ccatcgctga aggttcgttc
    36481 agcgccgagg cggtcagagt gtcgacaatg cgtttcgtca ggccagtaag cactttgccc
    36541 gggccgactt catagagatg ttctacgcct tgcgctgcca tgtactcaac agacttcgtc
    36601 cactgaaccg ggttatacaa ctgacgtacc agtgcgtcac ggatggcatc accattggtt
    36661 tcgcatttca catcaacgtt attcacaaca ggaactgttg gtgcgttaaa ggtgattttc
    36721 gctaattcta ctgccagttt gtcggctgct ggtttcatca gcgcacagtg agacggtacg
    36781 ctcactggta acggcagagc gcgttttgcg cccgcagctt tacaggcagc gccagcacgc
    36841 tcaactgctt ctttatgacc ggcaataacc acctgtcccg gagagttaaa gtttaccgga
    36901 gaaacgacct gaccttctgc agcttcttca cacgctttcg caatagacgc atcatccaga
    36961 ccgatgattg ccgccatagc gcccgtgcct tccggtacgg cttcttgcat gaacttgccg
    37021 cgcatctcaa ccaaacgcac cgcatcagcg aaatcaatta caccagcgca aaccagcgcg
    37081 gagtattccc ccaggctgtg accggccatc attgccggtg ctttaccgcc ctgctgctgc
    37141 catacgcgat acagcgcaac agatgcagtc aacagcgctg gctgagtttg ccaggtttta
    37201 ttcagttctt cagctggccc ctgctgggtc agcgcccaca gatcgtagcc cagcgccgca
    37261 gaagcttctg caaacgtttc ttcgacaatt ggatagctcg ccgccatatc agccagcatt
    37321 ccaacggttt gagaaccctg tccagggaac acaaatgcaa attgcgtcat gttttaatcc
    37381 ttatcctaga aacgaaccag cgcggagccc caggtgaatc caccgccaaa ggcttcaagc
    37441 agaaccaact gtcccggctt aatgcgcccg tcacgtacag cttcatccag cgcgcacggg
    37501 acagaggccg cagaggtatt accgtggcga tccagcgtca ccacgacatt gtccatcgac
    37561 atgccgagtt ttttcgccgt tgcactgata atacgcaggt tagcctgatg cggaaccagc
    37621 cagtccagtt gagaacggtc aagattattc gccgccagcg tctcatcaac gatgtgcgcc
    37681 agttccgtta ccgcaacctt gaagacttcg ttgcccgcca tcgtcagatg aattgaattc
    37741 tctggattca cacggtcagc attaggcagc gtcagcaatt caccatagct accgtcggca
    37801 tgcagatggg tggagatgat tcccggctct tcagaggcag ccagcaccgc agcgcccgcg
    37861 ccatcgccaa aaataataat agtcccacga tctgttggat cgcaggtgcg cgccagtaca
    37921 tcggaaccga cgaccagagc atacttcacc gccccagatt tcacgtattg atcggctacg
    37981 cttaatgcat aggtgaaacc tgcgcaggct gctgcaacgt caaatgccgg gcaaccttta
    38041 atgcccaaca tgctttgaat ctgacaagct gcgctcggga aagcgtgcgt cgcagaagtc
    38101 gttgccacaa cgatcaggcc aatctggtct ttctcaatac ccgccatctc aattgcgcgt
    38161 gtcgccgctt caaagcccat ggttgaaacg gtttcgtttg gcgcggcaat gtggcgttcg
    38221 cggataccgg tacgagtgac aatccactcg tcagaggtat ccaccatttt ttccaaatcg
    38281 gcgtttgtcc gcacttgttc gggcagatag ctgccagtac caataatctt cgtatacatg
    38341 tacgctcagt cacttttcgg ttatataccg tcacttgcaa actgcgagtt cgctggcagc
    38401 gtcctgctac cgcagagttc cgcttttgcc accgtccagc agctcaaaac cagctgggta
    38461 tacagattcc aggcgagcgg caattcgctg aggaacttgt cgctgcaccg cctgcactgc
    38521 ctgttcaatc gcgaccgcaa aagctcgctg attggctgca ccatgacttt ttatcaccgt
    38581 gccgcgcaat cctaacagac aggcgccgtt atactggtcg gggttgaggt gactgaatcg
    38641 cctcgtcagg ctcttttgta gccaacgctt taataacagt agccaccacg accgtttttt
    38701 cccttcaccc tgagatttca gcagagaaag gaacatcctg acaacacctt ccatcgtctt
    38761 taatgtgaca tttcctgtaa agccgtcaca aaccagcaca tctgtcttgc cagttaacaa
    38821 ctcattggct tcaagatagc cgatataatt gatagaaggg attgttttaa gcaccgctga
    38881 ggcatcccga atactgtcga gaccctttac ttcttcttca ccaatattga gcaacgccac
    38941 gcgaggatta ggaatttcca ccacctcttc agccagaact gagcccataa tggcaaattg
    39001 caccaacatt gtactgtcac aatcgacgtt ggcccctaaa tcgaggacca ccgttttgcc
    39061 cttttgctga tgcggtaata ccgtcaccag cgccggacgc tcaatcccct ccaggggctt
    39121 gagtaataat tttgccagcc ccatcagcgc cccggtatta ccggcactga cacaggcttg
    39181 cgctcgacct tctttcacca gctccagggc cacgcgcatt gaactcccac gactggcgcg
    39241 gatagcttgc gaaggccggg catcactggc gataactgac tgcgcaggaa taatctgcag
    39301 acgcgaacgt tgttcaaagt cagctttagc aagtaatggc gtgatggcgt cgggattgcc
    39361 gactaaaaga agagtgagtt gcgaattaga attcagtgcc tgcaatgctg caggcactgt
    39421 cacggaaggg ccaaaatccc ctcccatgac atctaacgcc agggttagac gtgtcaaggt
    39481 atcgtcgccg cccggtttgg tctttcccca gttgcctggg gaaaatcctc actaagcttc
    39541 atcacgcaga cgcgtgatta cttagcgatg accttgcggc cgcggtagta accgtcggca
    39601 gtgatgtggt gacgcaggtg tttttcacca gaagttttgt ctacagacag gctggtgact
    39661 gcggtcagcg cgtcatggga acgacgcatg ccacgtttgg aacgggttgg tttattctgt
    39721 tgtacggcca tggaccttac tcctcaatta cggtttatcc ccgatccaac ggggagcacc
    39781 aattacttac gctttaagct ggctaatacg gcaaatgggt ttggcttttg cgcttcttca
    39841 ggcagttcac caaagaccat gtccgcttcg gacacttcac agtgttcaga atcatgcacc
    39901 ggaactaccg gcaaggcgag gatgatttca tcttcaacca ttgcaagcag atcgatttca
    39961 ccgaattcgt taacctcaat cggttcatac gcttccggca gtgcttcagc ctgttcgtct
    40021 gaacgcacag gactaaaaca atacgttgtg tagacctgat gagtaaacgg cttcccgcaa
    40081 cgctgacatt cgagcgttac cgttaccttc gcatcaccag taatcacggc gagacgttgg
    40141 ttatcgatag cgaacgacat ggagcattcc acatcactgt ccacactgac cacagattcg
    40201 gcgacacgct caacctgatc cggagtataa ataccctggt aatcaaggcg tttttgagcc
    40261 gtgcgaaccg gatcaagagt caggggtaat tttacctttt gcatagggcg cgcatattaa
    40321 cttcgtaacg tcgtatagtc aaagaaaaag gcagcctgcg gttgcctttt gccaataatt
    40381 cgcacacatt gcgggttaca gccttatttt cgttcagtgc tggcaaagtt gccagtcagc
    40441 aaaacgaagc gcatagttta aattggctat catcaatacg ctatatctga tggaaaaaaa
    40501 tatgcctaaa cttattttag cctccacctc gccctggcgt cgcgctcttc tggaaaaact
    40561 gcaaatctct tttgaatgtg cagcacccga ggtcgacgaa actccacgca gcgacgaatc
    40621 accgcgacag ttggtgcttc gactggcaca agaaaaagcg caatctctgg cgtcatgtta
    40681 tccggatcat ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg
    40741 taaaccgtta acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt
    40801 caccttctat actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt
    40861 ggagcctttt gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg
    40921 taaagagcat cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct
    40981 gtttgagcgt ttagaggggc gtgatcctaa cacgctggtt ggtttaccgc taatcgcgct
    41041 gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg ggatagtttt ttgcgccgga
    41101 tgcggttact tcgcatccgg caatcggtgc tggcggcctg atgcggcgta aacgccttat
    41161 caggccaacc gcccattatc aggtttatat gcttgtatta gcgcgcgtta cgcagctttt
    41221 gcaaacaacg cttcaaacct tcatccatcg gcgcttcaat acgcatcacc tcaccggtcc
    41281 ccggatgagt aaacttcaac gctgcggcgt gcaggaacag gcgatttaat cccgtgcctg
    41341 cttcagtgag ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat
    41401 gacccgcata ttgtgtatgc acacggatct gatgagtacg ccctgttacc gggctacaac
    41461 gcaccagggt ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc
    41521 cttcctgact cacacgcacg atacgttcgc cgctttgcag aatatttttc agtaacggcg
    41581 cttgaacgct cttcacatac gactgccact gaccgcgcac cagcgccagg taatcttttt
    41641 gcatcccttt ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta
    41701 ccagcaacac acctgaggtg tcccggtcaa gacgatgaac cagttcaagg aaccgcgctt
    41761 ccgggcgcaa cgcccgcaaa ccttcaataa cgccgaagct taaaccactg ccgccatgta
    41821 ccgccgtacc ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt
    41881 ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa
    41941 cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac
    42001 gttttttgtt cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta
    42061 cgcctttcaa ttgggtacgc aaaaagttgt cgatacgttg ccccgcttcg tcagcggtga
    42121 tagcaacaat ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa
    42181 caggattcgc gccactcatt tttctatgct tatatttagt tttacacctt attacttcac
    42241 taggtgatca ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt
    42301 gagtaagcgg gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata
    42361 atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt
    42421 tgcccgaccg atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag
    42481 cgggctgcgg gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag
    42541 tctgtttctc tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc
    42601 gcctctttga gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg
    42661 ttcacacccg gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag
    42721 taagttacga tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc
    42781 cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa
    42841 aaggcaaaca tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt
    42901 gttgattacg gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat
    42961 ttccctgcta actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt
    43021 caggaagtca ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc
    43081 acctttatca gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggc
    43141 ggtatttctc gtcgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcaagc
    43201 cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct
    43261 gaggcgctgc aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc
    43321 gctgaaagcc gcccggctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca
    43381 ttccgcgatt acttacgcca ggacatcggt gaaatcctta tcgataaccc gaaagtgctc
    43441 gaactggcac gtcagcatat cgctgcatta ggccgcccgg atttcagcag caaaatcaaa
    43501 ctgtacaccg gcgagatccc gctgttcagc cactaccaga tcgagtcgca gatcgagtcc
    43561 gccttccagc gtgaagttcg tctgccatct ggtggttcga ttgttatcga cagcaccgaa
    43621 gcgttaacgg ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa
    43681 accgcgttta acactaacct cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt
    43741 gacctcggcg gcctgattgt tatcgacttc atcgacatga cgcctgtacg ccaccagcgt
    43801 gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc
    43861 catatttctc gctttggcct gttggaaatg tcccgtcagc gcctgagccc atcactgggt
    43921 gaatccagcc atcacgtctg cccgcgctgc tccggtaccg gtaccgttcg tgacaacgaa
    43981 tcgctgtcgc tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacacccag
    44041 gaagttcacg ccattgttcc tgtgccaatt gcttcttacc tgctgaatga aaaacgttct
    44101 gcggtaaatg cgattgaaac tcgtcaggac ggcgtacgct gtgtgattgt gccaaacgat
    44161 cagatggaaa ccccgcacta ccatgtgctg cgcgtgcgca aaggggaaga aaccccaacc
    44221 ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag
    44281 ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat gccggatgtg
    44341 ccgccagcac caacgccagc tgaacctgcc gcgcctgtcg tagccccagc acctaaatct
    44401 gcaccggcaa caccagccgc tcctgcccaa cctgggctgt tgagccgctt cttcggcgca
    44461 ctgaaagcgc tgttcagcgg tggtgaagaa accaaaccgt ccgagcaacc aacaccgaaa
    44521 gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt
    44581 gaccgtaatg agcgccgcga ccctcgtagt gaacgtactg aaggcagcga taatcgcgaa
    44641 gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt
    44701 cagcaggcag aggtaacgga aaaagcgcgt accaccgacg agcagcaaac gccgcgtcgt
    44761 gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagt gaaggcgctg
    44821 aatgttgaag agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag
    44881 ccacgtcgta aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa
    44941 gaagcggtag tcgcaccggt ggttgaagaa actgtcgcag ccgaaccaat tgttcaggaa
    45001 gcaccagccc cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca
    45061 ccagaacagc cagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt
    45121 tctcgccgct cgcctcgtca cctgcgcgta agtggtcagc gtcgtcgtcg ctatcgtgac
    45181 gagcgttatc caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg
    45241 gcctctggca aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaggttgaa
    45301 gagcagcgcg aacaggaaga agtacaagtg cagccgatgg tgactgaggt ccctgtcgcc
    45361 gccgctgccg aacctgttgt tagcgcgcca gttgttgaag aagtggctga agtcgtagaa
    45421 gcccccgttc aggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg
    45481 attgctgccg cggtaactga acagccgcag gtgattaccg aatctgatgt tgccgtagcc
    45541 caggaagttg cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt
    45601 gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct
    45661 gccgcgccag tcgtcgctga agtcacagca gaagttgaaa cggtagctgc ggttgaacct
    45721 gaggtcaccg ttgagcataa ccacgctacc gcgccgatga cgcgcgctcc ggcaccggaa
    45781 tatgttccgg aagcaccgcg tcacagtgac tggcagcgcc ctactttcgc cttcgaaggt
    45841 aaaggtgccg caggtggtca tacggcaacg catcatgcat ctgccgctcc tgcgcgtccg
    45901 caacctgttg agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt
    45961 tatttcatct ttgagtcaac atattaaaat tcatcaaatg gcatccttgc taaccaacaa
    46021 tacaaaatag gcaaaaaaat gcccgccccg gcgatcccag agcgggcaga caaaacatac
    46081 ccagtttcat gatatgtttt aaagcgagct tatttgttga gctggaagag cgacaatccc
    46141 tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat
    46201 gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa
    46261 gcgcgatcgc tacctaatga atccagcgac tccagttcgt tcagttgcgt gcctaattcc
    46321 gcgcgaacag tcagcacatt gttcagcgag tttttcagtc cgcggttggt tttatccagt
    46381 gccgcagcgg cggtttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg
    46441 atggcgctat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct
    46501 accgcgttgc tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa
    46561 cgcgaagcat cgacctgttg tttaatactt tctgttccgc cgacataatc accgtcagcc
    46621 tcactaaacg gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc gttaccgtca
    46681 gtggtgttcg ccagattcag taactggtca cgaagcccct gaatatccgt agccagcgag
    46741 gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga caattttttc ctgagcattc
    46801 tggatagcag tggtgacctg gctaagcaca ctctcttcca gtgacacttt ttgagtggcg
    46861 aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aacaacagct
    46921 tgtgatgcag caatgggatc atctgaaggg ttaacgactc gcttacccgt cgacatctgt
    46981 tcgccgtact tcatccattc tgcctgagaa ttggtgatgc cacgcatgtt ttgctggtac
    47041 atcatctggg tactgaaacg cattttatct ccccttagcg aatgttaatc agcgcatcaa
    47101 aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt
    47161 gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa
    47221 gctgcgtcac cacattacct tgcgtggcgc tactggtttt caacgtcgcg gttttattcc
    47281 cgatatcact cactaatgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt
    47341 tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta tcacccgcat
    47401 cttctgcgct cgccatggcg attttcgctt cgtcggtgat taatacatcc atgttgacga
    47461 tggcgtcact taccggtttc agtgttaagc tgtcgttaac ggcaggcgtt cctgtaaacg
    47521 tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg
    47581 tattactggc aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg
    47641 tcgccagtac cgcggaggca tcagttaccg tggcaccgat cgcaacgtca ccgttgtttt
    47701 tcgtgttttg cagtaccgcg ggcttaccga tagcaaagaa atcttcaccg gcatcgccgt
    47761 tagcatcaaa tccggctttg tgttgggtgt tgaaagcctc ggcaaatgcc agcgccagtt
    47821 gtccaagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga atgccgccca
    47881 gcgacccggt attcagtaat ttctccggga tctcaatatt gccagccgtc ccatcaatat
    47941 aagcgacagt cgtacgcgaa gggtcggcgg tggaaggaac tgccgccagc tgccgcgccg
    48001 tacttccctg aaccagtgag tagccattgg ccatcgtgat gttataagtg ccaccatcct
    48061 gaacgctaac ttctacgcca acaatctggt ttaattcact caccagttga tcgcgttgat
    48121 ccagcagatt gttaggtgac gcccctgccc ccacgcctgt cagacgcgag atttgatcgt
    48181 tcaggctggc gatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga
    48241 tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca
    48301 acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttacttacca
    48361 gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg
    48421 agagcatatt gtcgattttc gacatctgct catagcgggc agtcaggccg ctactttgcg
    48481 tctgcgccgc acgtaactgg ttggtaataa atgcatcata ctctcgctgc acaccagaaa
    48541 cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa
    48601 tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat
    48661 ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg
    48721 aggttcctta taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt
    48781 tattcagaac agattatcaa tgttcatact gtaggttttg ctcactttgt cgcttatcga
    48841 tttcatctgc tgaatcatgt tggtgagttt gcgggcatag tgaggatcgg tggcatagcc
    48901 cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca cggcagcgta
    48961 gcgcggatta cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta
    49021 gacccgaaat ttcgctttta ctttcttcgc ttcgccgttt tcatattcag tcgtggtgat
    49081 ttcagtgact ggccctttcc agttgccaga ggctttgacg ccaaacaggt tataactcgg
    49141 ctcgccgttt tcgcggcgga tttgccgttg cccccagcca gattcaagcg ccgcctgagc
    49201 gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagtgagag
    49261 ttgcgcgagg aatgctttac tgttacccgg caacgaatca tcgtagttac gtggtacggc
    49321 cttttgcacc agctgagaaa gcgcctgatt ttgataacgc accacagttt cgagcgggaa
    49381 tttcatcggt gctgctggcg tgggctcctc tggcaatggt tgttctggcg tcatctgttt
    49441 aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatctgctg
    49501 gtcatacata ctggtataca ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa
    49561 agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct ggcgggccac
    49621 cggacggata tttgccgctg gatcttcgcc agctttcgcc tttagttcgt tgagtgattg
    49681 cgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt
    49741 tttgcccgca gacatcccgc actttgcatt gattgcaaaa tagacatcag atccatcggc
    49801 gtagcgccca gcgcattgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg
    49861 ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc
    49921 tgtccaccac caaacggtgt atctggctgg ctgacattgg cctgacgatt gaccgtaacc
    49981 gagagattcc catgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc
    50041 gaaccggtac gcgagttaat cactactttg gcgtcctgcg gggtgacatt aacctgcata
    50101 ttctggatat cggcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga
    50161 atagtccgcg catctaacgc ggtggcgctg ccatatccac gcacgcgatt gatggtgtca
    50221 gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg
    50281 acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg
    50341 ttcagttggt ttacctgaac actgctaccg ccagcagagg ctcctgcgcc gccaaccaga
    50401 atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg tgtcatcaac
    50461 aacgtaccgc cacgcaagct tttggcatta cccatggaag aaaccaccac atcgatggtt
    50521 tgcccctggc gtccaaacgg tggaagtgac gctgtcacca taaccgcagc gacgtttttt
    50581 agctgcatat tagtgcccgt cggaacggta attcccagct gtgaaagcat gttattaagc
    50641 gtttgtgtgg taaacggcgt ctgagttgtc tggtcacctg taccatcaag ccccaccacc
    50701 aggccatagc caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata
    50761 cgctcagcct gagcagccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc
    50821 accacagcct cacttacatt ggcgacaggt tgaggaagaa acgctgcaac caacccatat
    50881 tttgcgcttc gttaatgtag ccattgccta cgtattcaat acgcgcatcc gccacctgag
    50941 tagacggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa
    51001 taaattcggt accctgatta atggcgatct gtttttcacc caccacatgc agattgccgt
    51061 tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct aaaggtatta ctggcattgg
    51121 ccccgccttt tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac
    51181 caaacagccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac
    51241 ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca
    51301 gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa
    51361 tcggctgagc agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt
    51421 gtgcactggt cgccccctgt accagcggcg tggagggtat ccaggcgcag ccggttagtg
    51481 aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca
    51541 tcttcaaaaa tcagtaaacc ggtgaatctg ccaccggtta tgccttagag ttgcgtcagt
    51601 ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc
    51661 acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct
    51721 tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc accggaggat
    51781 tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagtc cggtgtcatt cataaaggtg
    51841 gtgagattga gctgcccaac ctgaactgga gctgcctggc cttgttgggt tacgctgacc
    51901 acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc
    51961 acctgaaaac caccggccgt caccagctgc ccgttctgat ccacctggaa agagccgtca
    52021 cgggtatagg ctgatgaacc atctggcaac atcacctgga aaaagccctg ccctttaatc
    52081 gcgacatctt tgctgttgtt ggtctgcgac aggttgccct ggctgtgtaa gcgttcagtg
    52141 gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt ttgttcggaa
    52201 gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac cgcgcgctga
    52261 cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc catattggtt
    52321 tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa atgtcctgta
    52381 gtggattagt gacaaagtgc atatgtcttc ttgccggatg cggcgtaaac gccttatccg
    52441 gcctacaagt gctcgcaaaa tcgataaatt gcgattgccc ccatagaccc gataaacttg
    52501 cgcatcgggc aacttatact tgtcatgtat cctttcaatt aactcatcga tagcagttgg
    52561 ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc
    52621 cgtgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact gccttccaga
    52681 acccccgaca tcacacgcaa ggttggatct gcctgcagta ccggcccacg cgtagcctgg
    52741 ctttctgcgc ttaaacgaaa aatgccatcg tcaccgcgct ggacttcgct gcccgtggct
    52801 ttcaccagct tcagacgccc cactggcgca accgtatttg ccggatcgcc cggattcagc
    52861 gccgagattg tgccatcggc agcaatagtg atttccgccc cttccggcac agcaattggc
    52921 cccgcctcgc ctaccaccgg atgtccctga attgtcagtt gcccggtggg atcaacctga
    52981 atgctgccat tacgcgtata cccttcactg ccgtcagcgg tctgcacggc cagccaacca
    53041 tcctgctgca acgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc gggtgtcatg
    53101 tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc
    53161 ggcactgcgc gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga
    53221 ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcggtataa
    53281 attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca gcgtattgag
    53341 gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct gggcaacgat
    53401 catattgacc agttctttac tgagatcgac gttggacgct tccagcgcac cgttggtcag
    53461 tgtgccaaag ttgcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtagc
    53521 ggaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac
    53581 aatctgcccc agcagttggg tttgttcgtt ggaatagttg ccgacaaccg taccgtcatc
    53641 attgatttga taactcacca gatcgcccgg tttgtagccg ttctgggtgg ttgccacaat
    53701 gttgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa acgtggcggg
    53761 atctgcgccg ttaattgcgc cagtggaaat attcgctgtt ggatttgagg tcagaacgcc
    53821 attggcatta aacaccagtg ttgtcgctgt cttcgcaatg ctgtttggat cactactatc
    53881 ctgggtatag acctgccagt tattatcccc ggtcttcaca aagtagacgc tcatgtcatg
    53941 agcattaccc tgactatcga aaacagtcac cgaacctttt ttgttatagc tatccgcatt
    54001 gctggcatca aatgtgttaa cagagggaag cggatcactg gaattcaggt tgatctgcat
    54061 cgacgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa tattggtcgg
    54121 attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaccccg tcagctgtaa
    54181 accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt tacggctgta
    54241 gaacaccgaa ccattgctgt ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc
    54301 cagaccacgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga taccagcaac
    54361 ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga
    54421 tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga ggttggtggc
    54481 ggcaacgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct gaaatgtgaa
    54541 ggcttaaatt atctgccgta cttcgtcgag ggtagtggtg ccgtaagtgc caagatccag
    54601 cgtattaccg ttgttgccgc ggatcacccc ctgcaccaga gcaaactgca gcggttgggc
    54661 aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac cgttcggcgc
    54721 agtgctgccg tcagtcaacg taccgtccca ggtgaaactg tggactccgg cagtcagttc
    54781 accgatatca atggtacgga cgaccgcgcc gtttttatcg gtgatggtgg ccgtcacttt
    54841 gtctgccgcc tgttgcaact caacaccaaa cggcgtggtc gtggtcacag ccccttcttc
    54901 actgccggtt cccgcaagaa cagtggtacc ggggatcatc acgccgtgac caatcaggtt
    54961 actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccaa gcgtggtatt
    55021 gagtttttca atcccactga ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt
    55081 ttccattgga ttggtcgggt cctggttttt cagctgcgcc accaacaaag tcagaaaact
    55141 gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc
    55201 ggtattcgtc ggatcggtgg tggttaccgc aatggacata gctttctcct ttattgaccg
    55261 agcgtaaggg ttttcagcat catacttttc accgtgttga gcacttcaac attggcctga
    55321 taactgcgtg acgccgacat ggtgttaacc atctctccga cgacatcaac gtttggcatt
    55381 ttcacgtagc cctttgcatc cgccagcgga ttacccggtt cataaaccag tttgtccggg
    55441 gcctgacttt ctataacatc ggcaaccttt acgccgcctg tcgcagcacc tggcgcagcg
    55501 ttaacctgga ataccacctg ttttgcccga tatggctgtc catcgggacc ggtgacgcta
    55561 tcagcattcg ccagattact ggccgccacg ttcaggcgct gggactgggc agttaacgcc
    55621 gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct ctgtaaaacg
    55681 ttcatcatgc ctttgatctg cccgctcaac gcgctaaggc tcatctggta ttgcaggctg
    55741 ttatcggcaa actgggtgcg ttcgcgatcc atatcgacag tattaccgtc aagcgaaggc
    55801 tggtccggaa tacggtattg cagttctgcg gaaggaggcg tcagcgcctg cgccggaatg
    55861 tgtttcgttg aggtcatcgt cagagcaacc acactggttg catcccgtcc acgttgcatg
    55921 acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg ggtatcggca
    55981 ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag cgcctcttgt
    56041 tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt atcaaaattc
    56101 ggccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc
    56161 gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta gaaatggctg
    56221 gggacgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact gttcagtccg
    56281 ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc ccaactcgcg
    56341 ggggtaagtg atgaggttcg tgtttctatt cgtacagcac ccaatctgct accgccgtgc
    56401 gatcagccat tgctttcgat gagcaataat tcccgtctgt ggggcaatgt gaatgtgttg
    56461 gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac aggaaattat
    56521 gtggttgccg cgatgcccat tgtgcgtgga ggaaagctgg aagctggcaa tgtcaaactg
    56581 aaacgcggac ggctggatac cttgccaccg cgtacggtgc tggatatcaa tcaacttgtt
    56641 gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac ccactttcgc
    56701 caggcatggc gggtaaaagc gggacaacgt gtcaacgtga tcgccagcgg tgatgggttt
    56761 agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa tgcgcgggtg
    56821 cgcatgatat cgggacaagt ggtcagcggc gttgttgatg cagatgggaa tattcttata
    56881 aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac acatgataaa
    56941 agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct gaagccagta
    57001 agcaccgttc aaccgcgcga aaccactgac gcgccggtaa caaacacccg ggcggcaaaa
    57061 acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa
    57121 cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaagctggc gattcgtaac
    57181 ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga agcgcagcaa
    57241 gacttgcaga gtaactgatc gtatgacgcg tcttgcagag atcctcgacc agatgtccgc
    57301 tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct atatggggca
    57361 gatcaacggc agccagttgc aatggattac agaacaaaaa agctctctgc tggcgacgct
    57421 ggattacctc gaacagttac gcaggaaaga acccaattca gcaaatagcg ttgatattag
    57481 tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga atcaacataa
    57541 cggctggtta ctggaaggac agattgaacg caatcaacag gcgctggaaa tgttgaaacc
    57601 gcatcaggaa ccgacgctat atggagcgaa cggtcagacc tcaacaaccc atcgcggcag
    57661 taaaaagatt tcgatctgaa gattatctcc ggcctgcact gcaggccgga atgcattgtt
    57721 acaccgtccg gcgggcaaac tctttaactt tgaagcccag taccgccagt gcagcgaagt
    57781 acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taaacgccag ggcatggtac
    57841 ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac atcaccagta
    57901 ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggggta aagattttct
    57961 gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga ccaatagaaa
    58021 gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat tgcgttaaaa
    58081 tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa taaaagccag
    58141 gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca attaacgccc
    58201 gctgggtcat cagcgcatca aaggcggtaa atttaccgta ctggaacagc gaaacggtta
    58261 acggaccgga aagaatgccc aacgcaaccg cacttggcag cgccaacagg aaacaaagac
    58321 gcagccccca gtccatcaaa cggttgtatt catcatgatt gccactggca aaacttttcg
    58381 acagcgacgg tagcaaaatg gtgccaagcg ccacgcccag cacaccggac ggaaactcca
    58441 ttaagcggtc ggcgtaatac atccacgaca ccgaaccgga agcaagaaac gaggcaaaaa
    58501 tggtgttgat gattaaggag atctggctca cagagacgcc aaggattgcc ggtcccatct
    58561 gtttcaccac acgcattgct ccggcatcgt ggaagttaat gcgtggcaaa accagcatgc
    58621 cgatcttctt cagatgaggt agctgataca ccagctgcag gatgccgccg accgtaacag
    58681 cccaggccag cgccagcacc ggtggattaa agtacggcgc ggcaaacaac gcaaaaccaa
    58741 tcatgctgat attgagcaat gtcggggcaa acgccggaat tgagaagcgg ttccaggtat
    58801 tcagaatcgc tcccaccagc gacgccagag agatcagcaa gatataggga aaggttatct
    58861 ttagtagctg gctggtcagg gcaaatttgt cggcagtgtc agcgaagcca ggcgcggtca
    58921 ccatgatcac ccacggtgca gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg
    58981 tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc tgcttgcttt
    59041 tatattctgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca aagatacggc
    59101 gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa
    59161 agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac atggtcatcg
    59221 agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag acccacgggc
    59281 aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt atggtgaatt
    59341 gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat cttgtcaacg
    59401 atacgttgcg ccagcacggc ctgttcgccg gcggtttgcg gaactgtctg attttgcacg
    59461 cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcgagcgt actctgccaa
    59521 ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc gcgcatatcc
    59581 gtaatgtcga tgagcgcacc atcagtcacg gcctgcacgg tttcacgctg acttccggca
    59641 cggcgatgca tacaggtggt aatctgcaaa ggaccagccg aaaaatggtg ctcggcaaac
    59701 agcatttcgc cggactcatt ggtcagcaac gtaccgcttt cgagagttgc attgccgcca
    59761 gacaaccaca gcgcggtatc aaccacatgc aaataatcat ccagcaacgt gaaataaaga
    59821 tcgtgtggcc cgacgctatt gctacgatgt ttatccattc ttagcgaggc tgcggtggcg
    59881 agttgctttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac
    59941 gtcagttttt tccgcgccgc cagttccacc agccgttcag catcgcgcag attttctgcc
    60001 agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac cacgtcaaag
    60061 tggctggtgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag gctggataac
    60121 gaatcggcat aaggaatgcg ccagctgtca caaattggca aggctttggc gcgcgtaggc
    60181 gaccacgcac cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa ccacgctttt
    60241 tgcgcgatgc cgcctaaccc cactacgccg atacgtaatt ttttcacttt aatctcccag
    60301 atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt ccagggcttc
    60361 gacgcgggcc tgtaaatcac cgtcagctgc gttcgacata tccgttaccg ccggcggatt
    60421 ctcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg gttcgcgggc
    60481 cagacgcacc acaaaaggac catcttcgcg actcgccagt tgttccagcg tcgactccac
    60541 ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt
    60601 ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc tcagtttcag
    60661 atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat taccaaaacc
    60721 gctcaccgtg cgtagataat gacgtttgac cagattatcc agctgttcct gcacttcaga
    60781 ttcactcaga ttcataaccg gttcacggtt cgttttctga ttacaggccg tgactacacc
    60841 attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca agcaaccaat
    60901 cacgcgagct tccagggcag taagttgata tttcatgaga ttctccttag cggccgggcg
    60961 tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca
    61021 gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa tcaccactac
    61081 gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg cgctgcatat
    61141 aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat ttttgcccaa
    61201 tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa aaattggcaa
    61261 cgccaataat ctctttttcg tccgggtcga ataagccaaa gtaaaaagct gaaccttgtt
    61321 tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa cagtggcttt
    61381 cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa taatccgcca
    61441 gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg gttaagcgca
    61501 cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc gtaacgtctt
    61561 taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag tccagaatgc
    61621 cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac gttttgataa
    61681 aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc
    61741 tcatctttta tttcccggga ggggaaatgt cccgcgtgtc gcaggcgagg aacctgggta
    61801 aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc
    61861 tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc ggtattgccc
    61921 tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca attgccgacc
    61981 gctttggtgc caaaccgatg attgtcaccg gtatgctgat gcgtgccgcc ggattcgcca
    62041 caatgggtat cgcccacgaa ccgtggctac tgtggttttc atgcctgctt tcgggactcg
    62101 gcggcacgtt gtttgatcct ccgcgttcgg cgctggtggt gaaattaatc cgtccacagc
    62161 agcgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg gtcattggcg
    62221 cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc acaggggcag
    62281 ttttgtttgt gctgtgtgca gcgttcaatg cgtggttgtt accggcatgg aaactctcca
    62341 ccgtgcgcac gcccgttcgc gaaggcatga cccgcgtgat gcgcgacaag cgttttgtca
    62401 cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg ctgatgctgc
    62461 caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg tatgccattg
    62521 aagcgtgtct gtcgttaacg ttgctctatc ctatcgcccg ctggagtgaa aagcattttc
    62581 gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg atgccggtgg
    62641 gcatggtcag cggtctgcaa caacttttca ccctgatttg tctgttttat atcgggtcaa
    62701 tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcagacgca agagctcgcg
    62761 gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt ggttatatcg
    62821 gtggcggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt ccgtggatga
    62881 tgctgggcat tattggcatc ttcactttcc ttgcgctggg ttggcagttt agccagaaac
    62941 gcgccgcgcg tcgtttgctt gaacgcgacg cctgatttga tactcccccc tgactcctcc
    63001 atactgaaag tgaactgata gccaaatgga ggagtcacgt gaagctatac atttacgatc
    63061 actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat
    63121 tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt caaaaacagg
    63181 ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggac atcgttcact
    63241 atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct gccattgaag
    63301 agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt tttgccaaat
    63361 cggcatttga tgagttttct actcctgccg cgcgcaaata tttcgtcgac aagaaagagg
    63421 ccagcgcggg taattctgcc gacctgctgg cccactctga cggtctgatt aagaatatca
    63481 gcgatgattt acgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac
    63541 tttcggaaga tgatattcag ctatttccgc tcctgcgtaa tctgacgcta gtagccggaa
    63601 tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag acacaaatca
    63661 atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt attcgcgggt
    63721 tgccgccgtt ttcagttttt tctgctggcg cgtttatcgc tggtgacaca tgctgcgttg
    63781 tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt tttatttgcc
    63841 gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca atacaccatc
    63901 accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa agatatcggt
    63961 ttacccggcg tggctgacgc ccatattgtt ctgacaaacc tgaccagcca aattggtcgc
    64021 gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa ctccctgttc
    64081 ggtagccaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa
    64141 aaaggcgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca accggaaaaa
    64201 atgcaaacgg tgatgcaaac gttgcttccc tatttaaacc aggcattacg caattacttt
    64261 aaccagcaac ctgcttacgt cctgcgcgaa gatggcagcc agggcgaagc aatggcgaag
    64321 aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt tactgattaa
    64381 tcacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc
    64441 tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac tgcaccatcc
    64501 caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga tggcgacatg
    64561 ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt aatgcccaat
    64621 ctggctccgc ccgtgaccac cgttgaggct gccgtggcgt atcgccagcg cattcttgac
    64681 gccgtacctg ccgggcacga tttcaccccg ctgatgacct gttatttaac agattcgctg
    64741 gatcctaatg agctggagcg cggatttaac gaaggcgtgt tcaccgctgc aaaactttat
    64801 ccggcaaacg caaccactaa ctccagccac ggcgttacgt cagttgacgc aatcatgccg
    64861 gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tacatggtga agtaacacat
    64921 gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat ggaaccgctg
    64981 cgccagcgcc tgactgcact gaaagtcgtt tttgagcaca tcaccaccaa agacgctgcc
    65041 gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca gcatctgatg
    65101 tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg tctacccatc
    65161 ctcaaacgca atatccacca acaggcattg cgtgaactgg tcgccagcgg ttttaatcgt
    65221 gtattcctcg gaacggattc tgcgccacat gcacgtcatc gcaaagagag cagctgcggc
    65281 tgcgcgggct gtttcaacgc cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag
    65341 atgaatgctt tgcagcactt tgaagcattc tgttctgtaa acggcccgca gttctatggc
    65401 ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt tgctgaaagc
    65461 atcgcactga ctgatgacac gctggtgcca ttcctcgccg gagaaacggt acgctggtcc
    65521 gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct gtataaataa
    65581 ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga aaacttctcc
    65641 attgccagca ggggctattc acgccctggc tggcgaactt tcccgccgta ttcagtatgc
    65701 gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcaaata atttatcggt
    65761 tattggcgcg acaaaagaag ataaacagcg cattagcgaa attctccagg aaacgtggga
    65821 aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt ttgccgggtc
    65881 gccccggctt ttttatttta cttgcgaatt gtgagtacaa aatttctaca cttcctcaaa
    65941 ataattgcac aatatgctaa aaaaatatga atgacatgct gttt
//
LOCUS       DADSZD010000003        64957 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.003, whole genome shotgun sequence.
ACCESSION   DADSZD010000003 DADSZD010000000
VERSION     DADSZD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64957
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.003"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(18..497)
                     /gene="folK"
                     /locus_tag="J1Z28_000131"
     CDS             complement(18..497)
                     /gene="folK"
                     /locus_tag="J1Z28_000131"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBA7715005.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW"
     gene            complement(494..1912)
                     /gene="pcnB"
                     /locus_tag="J1Z28_000132"
     CDS             complement(494..1912)
                     /gene="pcnB"
                     /locus_tag="J1Z28_000132"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBA7715006.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1951..2877)
                     /gene="gluQRS"
                     /locus_tag="J1Z28_000133"
     CDS             complement(1951..2877)
                     /gene="gluQRS"
                     /locus_tag="J1Z28_000133"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBA7715007.1"
                     /translation="MLPPYFLFKEMTDTHYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2914..3369)
                     /locus_tag="J1Z28_000134"
     CDS             complement(2914..3369)
                     /locus_tag="J1Z28_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding transcription factor
                     DksA"
                     /protein_id="HBA7715008.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3547..4251)
                     /gene="sfsA"
                     /locus_tag="J1Z28_000135"
     CDS             complement(3547..4251)
                     /gene="sfsA"
                     /locus_tag="J1Z28_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBA7715009.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITSDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4266..4796)
                     /gene="thpR"
                     /locus_tag="J1Z28_000136"
     CDS             complement(4266..4796)
                     /gene="thpR"
                     /locus_tag="J1Z28_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBA7715010.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4870..7299
                     /gene="hrpB"
                     /locus_tag="J1Z28_000137"
     CDS             4870..7299
                     /gene="hrpB"
                     /locus_tag="J1Z28_000137"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBA7715011.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQTLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWMDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMATLGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7393..9927
                     /gene="mrcB"
                     /locus_tag="J1Z28_000138"
     CDS             7393..9927
                     /gene="mrcB"
                     /locus_tag="J1Z28_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBA7715012.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLTNQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10147..12405
                     /gene="fhuA"
                     /locus_tag="J1Z28_000139"
     CDS             10147..12405
                     /gene="fhuA"
                     /locus_tag="J1Z28_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBA7715013.1"
                     /translation="MAPSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVAVGTRGASNTYDYLIIRGFAADGQSQNNYLNGLKMQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMIGYSFDHEFNDTFTVRQNLR
                     FAQNKVSQKSVYGYGMCSDPLYTKDQEALKASPCLSIPQSQWGHTLTRQYVIDNEKLE
                     NFSVDTQLQSKFATGSVDHTLLTGVDFMRMRNDIDSWFGYAGSVAPSDIYNLDRSDFD
                     FGAHPNPSGPYRVLLKQKQTGLYVQDQAQWDKVLVTLGGRYDWADQSSFNRDYGNKSE
                     RDDKEFTWRGGVNYLFDNGVTPYFSYSESFEPASQTDANGDLFAPSKGKQYEVGVKYV
                     PEDRPIVVTGALYQLTKTNNLMADPNGSLFSVEGGEIRARGVELEAKAALSASVNVVG
                     SYTYTDAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYG
                     DPANSFKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGA
                     ERQVVATATFRF"
     gene            12456..13253
                     /gene="fhuC"
                     /locus_tag="J1Z28_000140"
     CDS             12456..13253
                     /gene="fhuC"
                     /locus_tag="J1Z28_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBA7715014.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13253..14143
                     /gene="fhuD"
                     /locus_tag="J1Z28_000141"
     CDS             13253..14143
                     /gene="fhuD"
                     /locus_tag="J1Z28_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBA7715015.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAVYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14140..16122
                     /gene="fhuB"
                     /locus_tag="J1Z28_000142"
     CDS             14140..16122
                     /gene="fhuB"
                     /locus_tag="J1Z28_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBA7715016.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16157..17437)
                     /gene="hemL"
                     /locus_tag="J1Z28_000143"
     CDS             complement(16157..17437)
                     /gene="hemL"
                     /locus_tag="J1Z28_000143"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBA7715017.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17662..19050
                     /gene="clcA"
                     /locus_tag="J1Z28_000144"
     CDS             17662..19050
                     /gene="clcA"
                     /locus_tag="J1Z28_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBA7715018.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLA"
     gene            19107..19172
                     /locus_tag="J1Z28_000145"
     CDS             19107..19172
                     /locus_tag="J1Z28_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715019.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19165..19509
                     /gene="erpA"
                     /locus_tag="J1Z28_000146"
     CDS             19165..19509
                     /gene="erpA"
                     /locus_tag="J1Z28_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBA7715020.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19556..20179)
                     /locus_tag="J1Z28_000147"
     CDS             complement(19556..20179)
                     /locus_tag="J1Z28_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBA7715021.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20217..21017)
                     /gene="btuF"
                     /locus_tag="J1Z28_000148"
     CDS             complement(20217..21017)
                     /gene="btuF"
                     /locus_tag="J1Z28_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBA7715022.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21010..21708)
                     /gene="mtnN"
                     /locus_tag="J1Z28_000149"
     CDS             complement(21010..21708)
                     /gene="mtnN"
                     /locus_tag="J1Z28_000149"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBA7715023.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21792..23309
                     /gene="dgt"
                     /locus_tag="J1Z28_000150"
     CDS             21792..23309
                     /gene="dgt"
                     /locus_tag="J1Z28_000150"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBA7715024.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23439..24863
                     /gene="degP"
                     /locus_tag="J1Z28_000151"
     CDS             23439..24863
                     /gene="degP"
                     /locus_tag="J1Z28_000151"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBA7715025.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25018..26175
                     /locus_tag="J1Z28_000152"
     CDS             25018..26175
                     /locus_tag="J1Z28_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family transcriptional regulator"
                     /protein_id="HBA7715026.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26228..26614)
                     /locus_tag="J1Z28_000153"
     CDS             complement(26228..26614)
                     /locus_tag="J1Z28_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBA7715027.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26926..27750)
                     /gene="dapD"
                     /locus_tag="J1Z28_000154"
     CDS             complement(26926..27750)
                     /gene="dapD"
                     /locus_tag="J1Z28_000154"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBA7715028.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEALFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27781..30453)
                     /gene="glnD"
                     /locus_tag="J1Z28_000155"
     CDS             complement(27781..30453)
                     /gene="glnD"
                     /locus_tag="J1Z28_000155"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBA7715029.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLGKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30515..31309)
                     /gene="map"
                     /locus_tag="J1Z28_000156"
     CDS             complement(30515..31309)
                     /gene="map"
                     /locus_tag="J1Z28_000156"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBA7715030.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31677..32402
                     /gene="rpsB"
                     /locus_tag="J1Z28_000157"
     CDS             31677..32402
                     /gene="rpsB"
                     /locus_tag="J1Z28_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBA7715031.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32537..33388
                     /gene="tsf"
                     /locus_tag="J1Z28_000158"
     CDS             32537..33388
                     /gene="tsf"
                     /locus_tag="J1Z28_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBA7715032.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33535..34260
                     /gene="pyrH"
                     /locus_tag="J1Z28_000159"
     CDS             33535..34260
                     /gene="pyrH"
                     /locus_tag="J1Z28_000159"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBA7715033.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34410..34967
                     /gene="frr"
                     /locus_tag="J1Z28_000160"
     CDS             34410..34967
                     /gene="frr"
                     /locus_tag="J1Z28_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBA7715034.1"
                     /translation="MISDIRKDAEVRMNKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35059..36255
                     /gene="ispC"
                     /locus_tag="J1Z28_000161"
     CDS             35059..36255
                     /gene="ispC"
                     /locus_tag="J1Z28_000161"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBA7715035.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQSACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36444..37202
                     /gene="ispU"
                     /locus_tag="J1Z28_000162"
                     /gene_synonym="uppS"
     CDS             36444..37202
                     /gene="ispU"
                     /locus_tag="J1Z28_000162"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBA7715036.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37215..38072
                     /gene="cdsA"
                     /locus_tag="J1Z28_000163"
     CDS             37215..38072
                     /gene="cdsA"
                     /locus_tag="J1Z28_000163"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBA7715037.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLVTAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38084..39436
                     /gene="rseP"
                     /locus_tag="J1Z28_000164"
     CDS             38084..39436
                     /gene="rseP"
                     /locus_tag="J1Z28_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBA7715038.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39466..41898
                     /gene="bamA"
                     /locus_tag="J1Z28_000165"
     CDS             39466..41898
                     /gene="bamA"
                     /locus_tag="J1Z28_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBA7715039.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42020..42505
                     /gene="skp"
                     /locus_tag="J1Z28_000166"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42020..42505
                     /gene="skp"
                     /locus_tag="J1Z28_000166"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBA7715040.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42509..43534
                     /gene="lpxD"
                     /locus_tag="J1Z28_000167"
     CDS             42509..43534
                     /gene="lpxD"
                     /locus_tag="J1Z28_000167"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBA7715041.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQKIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43639..44094
                     /gene="fabZ"
                     /locus_tag="J1Z28_000168"
     CDS             43639..44094
                     /gene="fabZ"
                     /locus_tag="J1Z28_000168"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBA7715042.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44098..44886
                     /gene="lpxA"
                     /locus_tag="J1Z28_000169"
     CDS             44098..44886
                     /gene="lpxA"
                     /locus_tag="J1Z28_000169"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBA7715043.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44886..46034
                     /gene="lpxB"
                     /locus_tag="J1Z28_000170"
     CDS             44886..46034
                     /gene="lpxB"
                     /locus_tag="J1Z28_000170"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBA7715044.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46031..46627
                     /gene="rnhB"
                     /locus_tag="J1Z28_000171"
     CDS             46031..46627
                     /gene="rnhB"
                     /locus_tag="J1Z28_000171"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBA7715045.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDNRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46664..50146
                     /gene="dnaE"
                     /locus_tag="J1Z28_000172"
     CDS             46664..50146
                     /gene="dnaE"
                     /locus_tag="J1Z28_000172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBA7715046.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50159..51118
                     /gene="accA"
                     /locus_tag="J1Z28_000173"
     CDS             50159..51118
                     /gene="accA"
                     /locus_tag="J1Z28_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBA7715047.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51216..53357
                     /gene="ldcC"
                     /locus_tag="J1Z28_000174"
     CDS             51216..53357
                     /gene="ldcC"
                     /locus_tag="J1Z28_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBA7715048.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53414..53803
                     /locus_tag="J1Z28_000175"
     CDS             53414..53803
                     /locus_tag="J1Z28_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA7715049.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53868..55166
                     /gene="tilS"
                     /locus_tag="J1Z28_000176"
     CDS             53868..55166
                     /gene="tilS"
                     /locus_tag="J1Z28_000176"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBA7715050.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTQLIRPLLARTR
                     GELVQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLS"
     gene            complement(55215..55475)
                     /gene="rof"
                     /locus_tag="J1Z28_000177"
     CDS             complement(55215..55475)
                     /gene="rof"
                     /locus_tag="J1Z28_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBA7715051.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLALELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55462..55662)
                     /locus_tag="J1Z28_000178"
     CDS             complement(55462..55662)
                     /locus_tag="J1Z28_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBA7715052.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55828..56373
                     /locus_tag="J1Z28_000179"
     CDS             55828..56373
                     /locus_tag="J1Z28_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBA7715053.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56370..56792
                     /gene="arfB"
                     /locus_tag="J1Z28_000180"
     CDS             56370..56792
                     /gene="arfB"
                     /locus_tag="J1Z28_000180"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA7715054.1"
                     /translation="MIVISRHVAIPDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56806..57516
                     /gene="nlpE"
                     /locus_tag="J1Z28_000181"
                     /gene_synonym="cutF"
     CDS             56806..57516
                     /gene="nlpE"
                     /locus_tag="J1Z28_000181"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBA7715055.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(57672..58496)
                     /locus_tag="J1Z28_000182"
     CDS             complement(57672..58496)
                     /locus_tag="J1Z28_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBA7715056.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHPSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58549..60267)
                     /gene="proS"
                     /locus_tag="J1Z28_000183"
     CDS             complement(58549..60267)
                     /gene="proS"
                     /locus_tag="J1Z28_000183"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBA7715057.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELADKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60378..61085)
                     /gene="tsaA"
                     /locus_tag="J1Z28_000184"
     CDS             complement(60378..61085)
                     /gene="tsaA"
                     /locus_tag="J1Z28_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBA7715058.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61082..61486)
                     /gene="rcsF"
                     /locus_tag="J1Z28_000185"
     CDS             complement(61082..61486)
                     /gene="rcsF"
                     /locus_tag="J1Z28_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBA7715059.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61604..62419)
                     /gene="metQ"
                     /locus_tag="J1Z28_000186"
     CDS             complement(61604..62419)
                     /gene="metQ"
                     /locus_tag="J1Z28_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBA7715060.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62459..63112)
                     /gene="metI"
                     /locus_tag="J1Z28_000187"
     CDS             complement(62459..63112)
                     /gene="metI"
                     /locus_tag="J1Z28_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBA7715061.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63105..64136)
                     /gene="metN"
                     /locus_tag="J1Z28_000188"
     CDS             complement(63105..64136)
                     /gene="metN"
                     /locus_tag="J1Z28_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBA7715062.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKTRRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAITWLQEHH
                     VKVEVLGYV"
     gene            64324..64896
                     /gene="gmhB"
                     /locus_tag="J1Z28_000189"
     CDS             64324..64896
                     /gene="gmhB"
                     /locus_tag="J1Z28_000189"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBA7715063.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     VTPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15298 a        16111 c        17914 g        15634 t
ORIGIN      
        1 attcacacga aaaataatta ccacttatct aattcattaa agccttttgc ctgtagagct
       61 tcaactaatg ataagccatc aggaaatacc aattccggcg caatttcaaa cagtggccac
      121 agcataaagc cgcgattttt catatcgtag tgcggaacgg tcagacgttc ggtatttatt
      181 acttcattac caaacagcat gatgtcgaga tccagcgtgc gcggtcccca gcgttcagct
      241 ttacggacgc gaccttgctg caattcgata cgttgtgtgt gattgagtag ctcttcaggg
      301 gcaagagtgg ttttcagcgc gacggcggca tttaagtaat cgggttgatc ttgcggcccc
      361 agcggtgggg tgcggtaaaa cgaagaaaca gcaagaatgc ggctttctgg aatatcgcct
      421 aatgctttca gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca
      481 atatacgcca ctgtcatgcg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag
      541 tacgacgacg cggtgacggt tcttcatcca gttcgttaag catccctttt tggtctggtg
      601 gcgcggaaac ctggaactca ccccaccatt tcaccagacg ttgcagttca gcgttacgct
      661 caacttcggc tcgtaaggcc aacaggtcgt atgccgcacg gaacttagga tgctccagca
      721 gtttccatgc gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc
      781 gggttagcgt ggtcagccgt ttcgggatcg ccagtgaacg gcaggcttcg tccagcacgt
      841 cgttcatcgc cagtgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct
      901 gtgccgtctc cagcaatggg taccagaaca tggcggcaaa caggaacgcc gggttcacgc
      961 gcatatcgtt atggatacgc gtatcggtat tcttcagtac ctgctcaata atgcgttcca
     1021 tcgggctgtc gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca
     1081 gatgatattc acacagcagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa
     1141 gcgattcttc aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga
     1201 tcggttctgc ggtttccggg ctgatgcgca tgcccaattt ggcggcaaaa cgaaccgcgc
     1261 ggagcatacg taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa
     1321 cgccgtcttt cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tctgccacgc
     1381 tgtaatacag gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga
     1441 aaatgttgtc gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga
     1501 cgttaccttc gtgatgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatca
     1561 catgagccag acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct
     1621 gctctggcgt ggcgttggtg gtcacgtcaa aatctttcgg ctttttgcca agtaacaggt
     1681 cgcgcacgcc gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta
     1741 ccttcagggc attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca
     1801 ccgtcacctg tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct
     1861 tgcggcaaaa attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt
     1921 caggacaaaa aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt
     1981 tacaattgcc gactcaggca cggcggtcag cgtccagttt ttgacggctg actgaaggat
     2041 ttgctcgacg ctgaaatcct gccagtgtgt ttctacctgc tgacccagaa aatgaagtgc
     2101 cgcgattaac accgggcgcg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga
     2161 aagtttagcg ccttgtggat taagcgccag cggtaaatgg atgtaatctg gcactttcca
     2221 gccaaaaagc tggtacagcg agatttgccg caccgtcggt tcgatcaaat ctgccccgcg
     2281 gacaatttcg ctcacgccct ggaagtgatc atccaccacc accgccaggt tataggcaaa
     2341 caaaccatca cggcgatgaa taataaaatc ttcccgtgcc agtttttcgt cggcgtgaat
     2401 aatgcctcgc agcagatcag taaactgggt gaccggatgc tgctggcgga tacgtactgc
     2461 ggcgttgtct ggtccatgat gcaaatcccg gcaatgaccg tcgtaaatac cgccaatgct
     2521 ttgaatacgc gcacgcgtgc aggtgcagta ataacttagt ccttgctcat gtaaccaggc
     2581 gagtgcttcg cgataggcgt ggtgacgttg cgattgccag agaacgtcgc cgtcccagta
     2641 caggccgtaa tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg
     2701 cgggtcgata tcttcaatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata
     2761 gctgccaagc gcagcaatca gagaaccaaa atgaagctcg ccggaaggag agggggcgaa
     2821 gcggccaata tagtgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc
     2881 ccgcctgtgg taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat
     2941 ttcagccagc gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg
     3001 aataccgatt tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt
     3061 cagcgtcttc tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag
     3121 gctgaactct tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc
     3181 ctgcatatgt gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag
     3241 aatacgacgg aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg
     3301 atatggttcc accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc
     3361 ttcttgcatg ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg
     3421 aggccgctat aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac
     3481 aactgtgtga ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt
     3541 actctactac aatgtaaccg gtagtgattt tttaagagcc atgccttcgg cagaaagttc
     3601 cgctttgtaa gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta
     3661 tttctcatcg atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa
     3721 aaagataacc gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg
     3781 acctcgttca gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga
     3841 tttcacttca atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct
     3901 gcgttcggcg ccgtatttta cttcgctttt cagcgagcta tagcctgcca gctctgaaat
     3961 tgattcatta aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat
     4021 aattgcgccg ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt
     4081 gtctgaagtc gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt
     4141 attcgggcag tgtagcgtta attcgcgacc atcggatgtg atcacatcgg ctaaaaaacg
     4201 tttgtaacgc tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat
     4261 ccttgttatt gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca
     4321 aacgaggagg cgtaaagggt gaactccgtc accgtatacg accagttaaa acctggcggc
     4381 gggattgtca ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga
     4441 ttgctttgaa aacaaccgct gcgggcagcc tgtgagcgga gcatattcgc cagctggatt
     4501 aagccgcgag gcggctgtcg catccctaac cacaccacac gcgaacgcag ccattgaccg
     4561 gcgtcatcaa gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc
     4621 gccttctctt tctctgcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg
     4681 gcggcgaccg gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccagtg gataatctgt
     4741 tcgcggattt ctgcaggtaa gtcgatagca aagaacagcc gttgcggttc agacatgtga
     4801 ggcactcggt tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg
     4861 gagcgttttg tgtcgtcgtt gcccgttgct gccgtcttac ctgagttact tgctgccctc
     4921 gatggtgcat cgcaggtgtt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg
     4981 ccgctgcaac tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt
     5041 cgtctggcgg cgcgtaacgt tgcgcaacga ctggcggagc tgcttaacga aaagccaggc
     5101 gataccgttg gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa
     5161 gtagttaccg aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt
     5221 ggattggtga tccttgatga atttcatgag cgcagcttgc aggcggattt ggcgttggcg
     5281 ctgttactcg atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct
     5341 acgctggaca acgaccgctt gcagcaaacg ctgccagaag cgcccgtcgt catctcagaa
     5401 gggcgctcgt ttccggttga acgccgttat ttgccgctgc ccgctcatca gcgttttgac
     5461 gaagccgtcg cggttgctac cgccgaaatg ctgcgtcagg aaagtggatc attactgtta
     5521 tttttacctg gcgtcggaga aattcagcgt gtgcaggaac aactgacttc acgcatcggc
     5581 agtgatgtgt tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg
     5641 atcctcccgg caccgcaagg gatgcgtaaa gtggtgctgg cgaccaatat tgctgaaaca
     5701 agtttaacca tcgaaggtat tcgtctggtg gtggattgtg cccaggaaag ggtggcgcgt
     5761 tttgatccgc gtacagggct tacgcggttg attactcaac gcgttagtca ggcgtcgatg
     5821 acgcaacgtg ccgggcgtgc cgggcgtctg gagccgggga tctgcctgca tttaatcgcc
     5881 aaagaacaag cagaacgcgc tgccgcacaa agtgaaccgg agatcttaca aagcgatctt
     5941 tccggtttgc tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg
     6001 atggatcaac cgccaacggt gaatctactg gccgcgaaac gcctgttacg gatgctgggg
     6061 gcgctggacg gtgaacggct tagtgcgcaa gggcaaaaaa tggcaacgct gggtaacgat
     6121 ccgcgtttag cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca
     6181 aaaattgccg ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg
     6241 ttttcgcgca atcaacccgc ctggcagcaa cgtagtcagc aactgttaaa acgtttaaac
     6301 gtacgcggcg gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg agcgtatgcc
     6361 gatcgtattg ctcgccgccg tgggcaagag ggacgctatc aactggcgaa cggcatgggg
     6421 gcgatgctcg atgccgacga tgcgctaagc cgccacgaat ggttgatcgc accgttatta
     6481 ttgcagggca gcgcctcgcc ggatgcgcgg attttactgg cgctgccggt cgatattgat
     6541 gagttagtac aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac
     6601 gcgcaaggta cgctgaaagc ctggcgtcgg ctgcaaattg gtcagttgat ggtaaaagtg
     6661 caaccgctgg cgaaaccctc ggaagacgag ttgcatcagg cgatgcttaa cggcattcgt
     6721 gataaaggtt taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg
     6781 ttatgcgccg caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg
     6841 ttggcgacgc tggaaacgtg gctactgccg catatgacag gcgtacattc gctacgcggc
     6901 ctgaaatcac tcgatattta tcaggcactg cgtggattac ttgattgggt aatgcagcaa
     6961 cgtctggata gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt
     7021 cgttaccatg aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag
     7081 gccactaatc cgactatcgc ccaggggcgc gtaccgttgg tgctggagtt gctttcccct
     7141 gcccaaaggc cgctgcaaat cacgcgtgat ttgagcgcct tctggaaagg agcgtaccgt
     7201 gaggtgcaaa aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca
     7261 aacaccgcac cgacgcgacg aacgaaaaag tattcgtgaa gaccaaagca aatttgagag
     7321 atatcttctt ctgtcttgca acagaagaac agaaaatcgg gcttttgcgc ctgaatattg
     7381 cggagaaaaa gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg
     7441 cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat
     7501 tatgatgact atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg
     7561 cgtaagcctc gtggcaaacg cggctggcta tggctgctgc taaaactggc tatcgttttt
     7621 gccgtgctga tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat
     7681 ggcaaggtct ggcaattgcc cgcggcagtt tatggccgaa tggtcaacct tgagccagac
     7741 atgaccatca gcaagaacga gatggtgaag ctgctggagg cgacccagta tcgccaggtg
     7801 tcgaaaatga cccgtcctgg cgagtttacc gtacaggcca acagcattga gatgatccgc
     7861 cgtccgtttg atttcccgga cagcaaagaa gggcaggtgc gcgcgcgtct gacctttgat
     7921 ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt
     7981 cttgatccgc gcctgatcac catgatttct tcgccaaacg gtgagcagcg tctgtttgtg
     8041 ccgcgcagtg gtttcccgga tttgctggtg gatactttgc tggcgacaga agaccgtcat
     8101 ttctacgagc atgatgggat cagtctctac tcaatcggac gtgcggtgct ggcaaacctg
     8161 actgccggac gcacggtgca gggggcgagt acgctgacgc aacagttggt taaaaacctg
     8221 ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg aagcctacat ggcgctgatc
     8281 atggacgcgc gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc
     8341 ggtcagagcg gcgacaacga aattcgcggc ttcccgctgg cgagcttgta ttactttggt
     8401 cgcccggtag aagaattgag cctcgatcag caggcgctgt tggtgggtat ggtgaaaggg
     8461 gcgtccatct acaacccgtg gcgtaacccg aaactggcgc tggagcgacg taacctggta
     8521 ctacgtttgc tgcaacagca acaaattatt gatcaagaac tctacgacat gctgagtgcg
     8581 cgtccgctgg gtgttcagcc gcgcggtggg gtgatctccc ctcagccagc ctttatgcag
     8641 ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg taaaagatct ctccggcgtg
     8701 aagatcttca ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa
     8761 ggcattccgg cactgaagaa acagcgtaag ttgagcgatc ttgaaaccgc gattgtggtt
     8821 gttgaccgct ttagcggtga agttcgtgcg atggtcggtg gttctgagcc gcagtttgcg
     8881 ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact
     8941 tatctaacgg ccttaagcca gccgaaaatc tatcgtttga atacgtggat tgccgatgcg
     9001 ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac cgcagaacga tgatcgtcgt
     9061 tatagcgaaa gtggcagggt gatgctggta gatgcgttga cccgttcgat gaacgtgccg
     9121 acggtgaatc tgggcatggc gttggggcta cctgcggtta cggagacctg gattaaactg
     9181 ggtgtgccga aagatcaact gaatccagtt ccagcaatgt tgttgggggc gttgaactta
     9241 acgccaatcg aagtggcgca ggccttccag actatcgcca gcggtggtaa tcgtgctccg
     9301 ctttccgcgc tacgttcagt catcgcagaa gacggcaagg tgttgtatca gagcttcccg
     9361 caggcggaac gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag
     9421 gttgtacaac gcggtacggg acgtcagctt ggggcgaaat atccgaacct gcatctggca
     9481 gggaaaacag ggactaccaa caataacgta gatacctggt ttgcgggcat tgatgggagc
     9541 acggtgacca tcacctgggt cggccgtgat aacaaccagc cgaccaaact gtatggtgcc
     9601 agcggggcaa tgtcgattta tcagcgttat ctgactaacc agacgccaac gccgctgaat
     9661 cttgttccgc cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc
     9721 agcggtggca tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag
     9781 agtgagatgc agcagcaacc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa
     9841 cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg
     9901 attaaagata tgtttgggag taattaacgt ctaagcgtga tataccggat ggcgagctgc
     9961 catccggtaa aataacatcc cctccaaagt attaaccctt tcttttcatc tgattgttta
    10021 ttgacccttc aggaatgcgc agattgcgta ccgcttgcga acccgccagc gtttcgaata
    10081 ttatctcatc tttataataa tcattctcgt ttacgttatc attcacttta caccagagaa
    10141 ataccaatgg caccttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt
    10201 gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa
    10261 gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggccggct
    10321 gcaactattg cggcgcgaca gtcagctacc ggcactaaaa ccgatacgcc gattcaaaaa
    10381 gtacctcaat ctatttctgt cgtcaccgct gaagagatgg cgctgcatca gcctaagtcg
    10441 gtgaaagaag cgcttagcta tacccctggc gttgctgtgg gaacccgtgg cgcatctaat
    10501 acttatgatt acctgatcat ccgcggattt gcggcagacg gccaaagcca gaacaactat
    10561 ctgaatggcc tgaagatgca gggcaacttc tataacgatg cggttattga cccatatatg
    10621 ttggagcgcg ctgaaatcat gcgtggcccg gtttccgtgc tgtatgggaa aagcagccca
    10681 ggcggcctgt tgaacatggt cagcaagcgc ccgaccactg aaccgttgaa agaagttcag
    10741 tttaaggccg ggactgacag cctgttccag accggttttg acttcagcga tgcactggat
    10801 gatgacggcg tttattctta tcgcctgacc ggtcttgcgc gttctgccaa tgcccagcag
    10861 aaagggtcag aagagcagcg ttatgctatt gcaccggcgt tcacctggcg tccggacgat
    10921 aaaaccaatt tcaccttcct ttcttacttc cagaacgagc cggaaactgg ttattacggc
    10981 tggctgccga aagaggggac cgttgagccg ctgccaaacg gtaagcgtct gccgacagac
    11041 tttaacgaag gggcgaagaa caacacctat tctcgtaacg agaagatgat tggttatagc
    11101 ttcgaccacg aatttaacga cacctttact gtgcgtcaga acctgcgctt tgcgcaaaat
    11161 aaagtttcgc aaaagagcgt atatggctac ggtatgtgtt cagatccgct gtataccaaa
    11221 gaccaggagg cactcaaggc gagtccatgt ttaagtatcc cgcagtctca atggggccat
    11281 acactgactc gtcagtacgt tatcgataat gagaagttag aaaacttctc tgttgatact
    11341 cagttgcaaa gcaaattcgc aaccggttct gtggatcaca ctctgctgac tggcgttgac
    11401 tttatgcgta tgcgtaatga catcgactcc tggttcggat atgccggttc agttgcacca
    11461 tctgatatct ataatttaga ccgtagtgac tttgattttg gcgctcaccc gaatccgtct
    11521 ggtccatacc gcgttctgct gaaacagaaa caaaccggcc tgtatgttca ggatcaggcg
    11581 cagtgggata aagtgctggt tacgcttggc ggtcgctatg actgggccga tcagtcatct
    11641 ttcaaccgtg attacggtaa taaatctgaa cgtgacgaca aagagttcac ctggcgtggt
    11701 ggtgtaaact acctgttcga caacggcgta acgccttact tcagctacag tgaatcattt
    11761 gaacccgctt cacagactga tgcgaacggt gatctgtttg caccttcgaa aggcaaacag
    11821 tatgaagtcg gcgtgaaata tgtgccagaa gatcgcccaa ttgtggtgac tggcgcactg
    11881 tatcagctta ccaaaactaa taacctgatg gcggatccga acggttcact gttctcggtt
    11941 gaaggcggtg agattcgtgc gcgtggcgta gagctggaag cgaaagcggc actgtcggcg
    12001 agtgttaacg tagtcggttc ttatacttac accgatgcgg aatacactac cgatactacc
    12061 tacaaaggca atacgcctgc acaggtgcca aaacacatgg cttcgctgtg ggctgactat
    12121 accttctttg acggtccgct ttcaggtctg acgctgggca ccggtggtcg ttatactggc
    12181 tccagctatg gtgatccggc taactccttt aaagtgggaa gttatacggt cgtggatgcg
    12241 ttagtgcgtt atgatctggc gcgagtcggc atggcgggct ccaacgtggc gctgcatgtc
    12301 aacaacctgt ttgatcgtga atacgtcgcc agctgcttta acacttatgg ttgcttctgg
    12361 ggcgcagaac gtcaggtcgt tgcaaccgca accttccgtt tctaatttct cttttggggc
    12421 acggatttcc gtgcccgttt cacaagttgg ctgttatgca ggaatacacg aatcattccg
    12481 ataccacttt tgcactgcgt aatatctcct ttcgtgtgcc cgggcgcacg cttttgcatc
    12541 cgttgtcgtt aacctttcct gccgggaaag tgactggtct gattggtcac aacggttctg
    12601 gtaaatccac tctgctcaaa atgcttggtc gtcaccagcc accgtcggaa ggggagattc
    12661 ttcttgatgc ccaaccgctg gaaagctgga gcagcaaagc gtttgcccgc aaagtggctt
    12721 atttaccgca gcagcttcct ccggcagaag ggatgaccgt gcgtgaactg gtggcgattg
    12781 gtcgttaccc gtggcatggc gcactagggc gctttggtgc ggcagatcgc gaaaaggtcg
    12841 aggaagctat ctcgctggtt ggcttaaaac cgctggcgca tcggctggtc gatagccttt
    12901 ctggcggcga acgtcagcgg gcgtggatcg ccatgctggt ggcgcaggat agccgctgtc
    12961 tgttgctcga cgaaccgact tcggcgctgg atatcgccca ccaggttgat gtactggcgc
    13021 tggtgcatcg tttaagtcag gagcgaggcc tgacggtcat tgccgtgctg cacgatataa
    13081 atatggcggc gcgctactgt gattatctgg tcgctctgcg cggcggtgaa atgattgctc
    13141 agggaacgcc tgcggaaatt atgcgcggcg aaaccctcga aatgatttat ggcatcccga
    13201 tgggtattct gccgcatccg gcgggtgctg cacctgtgag ttttgtttat tgatgagcgg
    13261 cttacctctt atttcgcgcc gccgactgtt aacggcgatg gcgctttctc cgttgttatg
    13321 gcagatgaat accgcccacg cggcagttat tgatcccaat cgtattgtgg cactggagtg
    13381 gttgccggtg gagttactgc tggcgctcgg catcgtgcct tacggcgtgg cggacaccat
    13441 caactatcgc ctgtgggtca gcgaaccgcc attgccggac tcagtgattg acgttggttt
    13501 gcgtacagaa cccaaccttg aactgctgac cgaaatgaaa ccgtcgttta tggtctggtc
    13561 tgcaggatat ggcccttcac cagaaatgct ggcacgtatt gcgccggggc gcggatttaa
    13621 cttcagcgac ggcaaacagc cactggcgat ggcgcgtaaa tcactgacgg aaatggcaga
    13681 tttacttaac ctgcaaagcg cagcggaaac gcatttagcg cactatgaag actttatccg
    13741 cagcatgaaa ccccgctttg tgaagcgtgg tgcacgcccg ttattgctga cgacgcttat
    13801 cgatccgcgc catatgttag tcttcggtcc aaacagcttg ttccaggaaa ttctcgatga
    13861 gtacggcatc ccaaatgcct ggcaagggga aaccaacttc tggggcagta ccgccgtcag
    13921 tatcgatcgt ctggcagtgt ataaagacgt tgatgtgctc tgttttgatc acgacaacag
    13981 caaagacatg gatgcgctaa tggcaacccc gctgtggcag gccatgccgt ttgtccgcgc
    14041 cggacgcttt cagcgcgttc cggcggtctg gttctatggt gcgacgctct cggcaatgca
    14101 ctttgtgcgt attctggata acgccatcgg aggtaaagcg tgagtaaacg aattgcgctt
    14161 tttccggtgt tattgctggc gctgctggtg gttgctgctg cagcgttgac ctggatgaac
    14221 ttctcgcagg cgctgccgcg cagtcagtgg gcgcaggccg cctggtcgcc ggatattgac
    14281 gtcatcgagc agatgatttt tcactacagc ttgttgccgc gtctggcgat ttcgctgctg
    14341 gtgggcgcgg gcctggggct ggtaggcgtg ctgtttcagc aagtgctacg taacccgctg
    14401 gcggaaccga cgacgttggg tgttgcaaca ggcgcgcaac tggggattac cgtcaccacg
    14461 ctctgggcga tccccggcgc gatggcgagc cagtttgctg cgctggcagg ggcttgtgtt
    14521 gttggcttaa tcgtctttgg cgtcgcgtgg gggaaacggc tttcgccggt aacgctgatc
    14581 ctcgcggggc tggtagtgag cctttattgc ggcgcaatca atcagttact ggttatcttc
    14641 catcatgacc aactgcaaag catgttcctg tggagcaccg gaacgctgac gcaaaccgac
    14701 tggggcggcg ttgagcgttt atggccgcag ctgctgggcg gcgtgatgct gacgttattg
    14761 ctacttcgcc cgttaactct gatggggctt gatgatggcg tggcgcgcaa tctcgggctg
    14821 gccttgtcgc tcgctcgtct ggcggcgttg tcgctggcga ttgtcatcag tgcgctgctg
    14881 gtgaacgcgg tggggattat cggctttatc gggttgttcg cgccactgct ggcgaaaatg
    14941 ctgggggcgc ggcgtttgtt gccacggttg atgctggcat cgctgattgg tgcgttaatt
    15001 ctgtggcttt ctgatcaaat catcctctgg ctgactcgcg tgtggatgga agtgtccacc
    15061 ggttcggtca ctgcgttgat cggtgcgccg ctgctactgt ggttgctgcc gcgtttacgc
    15121 agcattagcg cgccggatat gaaggtgaac gatcgtgtcg cgactgaacg ccaacacgtg
    15181 ctggcgtttg ccctcgcggg cggcgtgctg ctgttgatgg ctgtggtggt ggcgctgtcg
    15241 tttggtcgtg atgcgcacgg ctggacgtgg gcgagcgggg cgttgctcga ggatttgatg
    15301 ccctggcgct ggccgcgaat tatggctgcg ctgtttgcgg gcgtcatgct ggcggtggcg
    15361 ggctgtatta ttcagcggct gaccggaaac ccgatggcaa gcccggaagt gctggggatt
    15421 agctccggcg cggcgtttgg cgtggtgtta atgctgtttc tggtgccggg taatgccttt
    15481 ggctggctgt tacccgcagg cagtctcggg gcggcggtga cgctgttgat cattatgatc
    15541 gccgccggac gtggtggatt ttccccacac cgcatgttac tggcggggat ggcgttaagt
    15601 accgcgttca ccatgctttt gatgatgttg caggcaagtg gtgacccgcg aatggcgcaa
    15661 gttctgacct ggatttccgg ttcgacctac aacgcgaccg atgcgcaggt ctggcgtacc
    15721 ggaattgtga tggtgatttt gctggcgatt accccgctgt gccgccgctg gctgaccatt
    15781 ttaccgctgg gtggtgatac cgcacgagcc gtggggatgg cgctgacgcc gacgcgaatt
    15841 gcgctgctgc tgttagcggc ttgcctgacg gcgaccgcga caatgaccat tggaccgttg
    15901 agttttgttg gtttaatggc accgcatatt gcgcggatga tgggctttcg acggacgatg
    15961 ccacacatcg taatttcggc gctggtgggt ggtttactgc tggtgttcgc tgactggtgt
    16021 gggcggatgg tgctgtttcc attccagatc cccgcggggc tgctgtcgac ctttatcggc
    16081 gcgccatatt ttatctattt gttgagaaag cagagccgtt aattgaatgc ctcgcccggt
    16141 gggcgaggca aaatcatcac aacttcgcaa acacccgacg tgcagcatcg atggtgttat
    16201 tgatatcttc catgctgtgc gccacagaca taaagcccgc ttcaaacgct gacggtgcca
    16261 ggtaaacacc ttcgtccagc atcatatgga agaaacgctt aaagcgttcc acgtcacagg
    16321 ccatcacatc ctgatagcac gtcacggact cggcgtcggt aaagaaaata ccgaacatgc
    16381 cgccaacgtg gtttaccacc agcggaattc cggcttcttc tgccgcttcc cgcagacctt
    16441 ctgccagacg tgttgtcagc tcatccagcg tctcgtgaac gcccggctgt gcgacttcat
    16501 tcagacaggc gaaaccagca gccatcgcaa tcgggttacc ggaaagcgta cccgcctgat
    16561 agaccggacc ggtcggggcc agcgcatcca ttacatcacg acgaccaccg aatgcgccta
    16621 ccggcattcc accgccgatg attttgccga ggcaggtgag atccggttcc acaccgtaat
    16681 aatcctgtgc gccagctagc gctacgcgga aaccggtcat cacttcatcg atgatcagca
    16741 acgcgccaaa ttcgtcgcac agcgcgcgca gacctggcag gaactctggc agcggtggaa
    16801 cgcagttcat attgcctgcc accggctcga cgataataca ggcaatctct tgcgggtatt
    16861 gctcaaacgc ggcgcgtaca gaggccagat cgttataagt acaggttaag gtatgtttgg
    16921 cgaaatctgc cggaacgccc ggcgaatttg gctggcctaa cgtgagtgcg ccagaaccgg
    16981 ctttcaccag caggcagtca gcgtgaccgt ggtaacaacc ttcaaattta ataattttgt
    17041 cgcgaccggt aaaaccacgg gccaggcgga tggcgctcat cgtcgcttca gtgccggagt
    17101 tcaccatgcg caccatatcc atggtcggga ccagttcggt caccagttgc gccattttca
    17161 cttccatttc ggttggtgca ccaaagctta aaccacgctc ggcggcttca atcacggcat
    17221 tgcggatcgc cggatggtta tggcccagca ccatcggccc ccaggaaccg acataatcga
    17281 tataggcttt gccatcaaca tcgtacagat aagcgccgtc cgctttttcg ataaacagtg
    17341 gagtgccgcc cacgccagta aaggcgcgaa caggggagtt cacaccgcca gggatcagct
    17401 cgcgcgctgc gctgtaaaga ttttcagact tactcatgga gggttcctga ttcgtagaaa
    17461 aagtgaatgg ctgctattct atgttattca taacaagtta aatactcgtc aaacatcagg
    17521 ctgcttgtac tggtgcaatc ctgatttctt agagtataaa agttttgtgc atttgaaaca
    17581 ttacgctttg caaaggattt tcatggaacg tgcgagtaaa atgccgtcat cttatttgta
    17641 tgaccaataa gtgatcattg gatgaaaact gatactccct ctttagaaac accgcaggcc
    17701 gcgcgcctgc gacgcagaca actgattcgc caacttcttg agcgcgataa aaccccgtta
    17761 gccattttgt ttatggcggc agtcgtcggc acgcttgtcg ggctggctgc ggttgctttt
    17821 gacaaaggtg tcgcctggtt gcagaaccaa cgtatggggg cactggtaca tactgctgat
    17881 aattatccgc tactgttaac cgtcgctttt ctctgttcgg cggtgctggc gatgttcggc
    17941 tactttttgg tgcgcaaata tgcgccggaa gcaggtggtt cggggatccc ggaaattgaa
    18001 ggggcgctgg aagatcaacg tcccgttcgc tggtggcgtg tgttgccggt gaaattcttt
    18061 ggcgggctgg gaacactcgg cggaggcatg gtgttggggc gcgaagggcc aaccgtgcag
    18121 ataggcggca atatcgggcg gatggtactc gatgttttcc gcctgaaagg tgacgaagct
    18181 cgtcatacgc tgttggcaac cggtgctgct gcggggctgg ctgcggcctt taacgcgccg
    18241 ctggcgggta ttttgtttat tatcgaagag atgcgtccgc agtttcgcta tacgttaatt
    18301 tcgattaaag cggtatttat tggcgtcatt atgtcgacca ttatgtaccg gatttttaat
    18361 catgaagttg cgttgattga cgtcggtaaa ctttctgatg cgccgcttaa tacgctgtgg
    18421 ctttatctga tcctcggtat tatttttggc attttcggcc ctatttttaa taaatgggtg
    18481 ctggggatgc aggatttgct gcaccgtgtg cacggtggca atattaccaa atgggtactg
    18541 atgggcggtg cgattggcgg tctgtgtgga ttgctggggt ttgtggcacc agcaacttcg
    18601 ggcggcggtt ttaacctgat tcctatcgct accgcgggga atttcagcat gggaatgctg
    18661 gtgtttatct tcgtcgcgcg ggtcattacc actttactct gcttctcttc cggcgcgccg
    18721 ggcggtattt ttgccccgat gctggcgctg ggtactgtgc tgggaaccgc tttcggaatg
    18781 gttgccgttg agttgtttcc gcaatatcac cttgaggcgg ggacgtttgc tattgccgga
    18841 atgggggcgt tactggcggc atctattcgc gcgccgttaa cggggatcat tctggttctg
    18901 gaaatgaccg ataactacca gctcattttg ccaatgatta ttaccggtct tggcgcaaca
    18961 ctattagcac aatttaccgg tgggaaaccg ctatactcgg cgattcttgc gcgcacgctg
    19021 gcaaaacagg aagctgagca actggcgtga agcaaggccg catcagcccg cgagaatact
    19081 tgaacgaaat accagggtat tagataatgg cgattattat tgggttagaa tttgcccaat
    19141 tgccgatgtc gtttggagca aaatatgagt gatgacgtag cactgccgct ggagtttacc
    19201 gacgcagcag ccaacaaagt taaaagcctg atcgctgacg aagataaccc gaatctgaaa
    19261 ttacgcgtgt atatcaccgg tggcggttgc agcggcttcc agtatggttt cacctttgat
    19321 gatcaggtga acgaaggcga tatgaccatc gaaaaacagg gcgttggcct ggtggttgat
    19381 ccgatgagcc tgcaatatct ggttggcggt tccgttgact ataccgaagg tctggaaggt
    19441 tctcgtttca tcgtgaccaa cccgaacgcg aaaagtacct gcggttgcgg ttcttccttt
    19501 agtatctaat ctgctgttca tgtgccggat aaactatccg gcacatcttc tctcttcaac
    19561 gaccattctc atccagcgca aacgtcggta gcttaagatg ccaacgaata gccgccagcc
    19621 gaatcaatag cgtcacgacc atgcccatca tactggctgt ttccagtggt acggaaaatg
    19681 tgtaataagc cgtagcgtgg acaataccgc cgataataca ggcagttgcg tagatttctg
    19741 tacgtaaaat catggggatt tcgcgggcca gtacatcacg aatgatcccg ccgccaacgc
    19801 cagtaatgac gcccatacaa accgcgatta acggaccggc ttccgcatta aaggctttat
    19861 tcacgccaat gccgacaaac accgccagac caacggcgtc cagcaccggc aacatccatt
    19921 ttggtaagcg tcttggctgg cgcaccagca cgatggtcag catgctggtg accattgcaa
    19981 cgaccagatc ggtgggatct ttcacccaaa ataccgggcc gtgatccagc gccatgtcgc
    20041 gaattgttcc gccgcctact gcggtaacca cgcccaatac cagaacacca aaagggtcca
    20101 tacgcaattt tccggctaac aaaacgccag agatagcaaa taccgctgtg ccgactatat
    20161 ccagccaata gacgagcatt gttcaatccc cactgagcac ctgaaaaggt caggcactaa
    20221 tctacctgtg aaagcgcatt acagagctgt tgtgcagcga ggataatacg tgggcttgca
    20281 cgttcaaacc agtcactcgt gagaggaata acgggaattt tgagctgttc accccagtat
    20341 tgtttgattt taggaatttg gtccggtccg cctgtaatga caatcgcctg tggcgagcgt
    20401 gctaacacct gttcgcggct aacttgcggc cagggaaccc ggctgtcttt aaagatgttt
    20461 tctccgccac aaacttcgag tacctggttc tgaatcgact cttttccact ggtaaatggc
    20521 ggattaatgc cgaattgcag aaaaacacgt tttttaggtt tatcagcata ttgcgctttc
    20581 aattgcgcgt actgatccag cagggattgc gcggcttgtt cggccttatc cggttgcgga
    20641 ctccaggggg ccagttgacg taacgcattg gcaatttgtt caatgcttgt cgcatcgacc
    20701 cacatcactt ttattcccag cgaagccagc tggtcaacct gccgctcggc attacctcca
    20761 cgccaggcaa tcaccagatc gggtttcagc gcgacaatgc gttccagatt catcccctgc
    20821 caggtggaaa cctgctcaat cttttgcgct tgtggaggat agtcggaata gctgctgacc
    20881 ccaaccggcg tgatcccggc ggcaaaggca agttcagtgt tggcgggaga aagcgtgatg
    20941 acgcgcggcg cggcattgag ccacagcggc gcgagaaaag acagggcgac cagcgccctg
    21001 aacagtgact tagccatgtg ccagtttctg caccagtgac tcaaccatca ggctggactg
    21061 tttagcggca acagccagga actcatcgaa gctaagatga gactgttgat cggccacgtc
    21121 ggagatggcg cgtactacga caaacgggac gttgaaattg tggcagacat gggcgattgc
    21181 cgtcgcttcc atctctacag caatggcctg tgggaagttg tggcggattt tcgccagacc
    21241 aacagaaccg ttgatgaaag cgtcgccgct aacaatcagg ccacgtacag cgttaagatt
    21301 cagttcggca atgcaggcct cagcggcagc gatcagttta tcgtcagctt tgaagcctgc
    21361 cgggcagcct ggtaactgac cgtattcata accaaatgcc gtgacatccg cgtcgtgata
    21421 acgcgcttcg tccgagacaa cgatatcgcc cactttcagc gttggtgcca ggccaccggc
    21481 agaaccggtg ttaataatca catctggctt gcagtgttcc aacagcagag tggcacccag
    21541 cgccgcagcg actttaccga tgcccgattt cagaagcgca acctcggttc cattcagttg
    21601 gccggtatag atttcgcaac cgcccagact gatagtttga cggttttcga ttttgtcacg
    21661 cagcagcgta acttcttctt ccattgcacc aatgatgccg attttcatag atttactcgc
    21721 gataagcccg atttgaaggc atagtttacc atgcgcttac ggggaagcgt atttctcacg
    21781 cgggagagga catggcacag attgatttcc gaaaaaaaat aaactggcat cgtcgttacc
    21841 gttcaccgca gggcgttaaa accgaacatg agattctgcg gatcttcgag agcgatcgcg
    21901 ggcgtatcat caactctccg gcaattcgtc gtctgcaaca aaagacccag gtttttccac
    21961 tggagcgcaa tgccgccgtg cgcacgcgtc ttacccactc gatggaagtc cagcaggtgg
    22021 ggcgctacat cgccaaagaa attttaagcc gtctgaaaga gcttaaatta ctggaggcgt
    22081 atggtctcga tgaactgacc gggccctttg aaagcattgt tgagatgtca tgcctgatgc
    22141 acgatatcgg caatccgccg tttggtcatt ttggcgaagc ggcgataaat gactggtttc
    22201 gccagcgttt gtacccggaa gatgccgaaa gccagcctct gactgacgat cgctgcagcg
    22261 tggcggcact acgtttacgg gacggggaag aaccgcttaa cgcgctgcgg cgcaagattc
    22321 gtcaggactt atgtcatttt gaaggtaacg cacagggtat tcgtttggtg catacattga
    22381 tgcggatgaa tctcacctgg gcacaggttg gcggtatttt aaaatatacc cgtccggcgt
    22441 ggtggcgtgg cgaaacgcct gagacacatc actatttaat gaaaaagccg ggttattatc
    22501 tttctgaaga agcctatatt gcccggttgc gtaaagaact taatttggcg ctttacagtc
    22561 gttttccgtt aacgtggatt atggaagctg ccgacgacat ctcctattgt gtggcagacc
    22621 ttgaagatgc ggtagagaaa aggatattta ccgttgagca gctttatcat catttgcacg
    22681 aggcgtgggg ccagcatgag aaaggttcgc tcttttcgct ggtggttgaa aatgcctggg
    22741 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
    22801 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
    22861 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
    22921 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccagatgtcg
    22981 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
    23041 tattaaacct gccgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
    23101 tccctattga aacgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
    23161 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattatc
    23221 gttgccgcct gctgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
    23281 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
    23341 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
    23401 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
    23461 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
    23521 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
    23581 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
    23641 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gagggttctc
    23701 cgttccagag ctctccgttc tgccaggggg gcctgggcgg taatggcggc ggccagcaac
    23761 agaaattcat ggcgctgggt tccggcgtta tcattgatgc cgataaaggc tatgtcgtca
    23821 ccaacaacca cgttgttgat aacgcgacgg tgattaaagt ccaactgagc gatggtcgta
    23881 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccagatcc
    23941 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcgctg cgcgtgggtg
    24001 attacaccgt agcaattggt aacccgttcg gcctgggcga gacagtaact tccgggattg
    24061 tctctgcgct gggacgtagc ggcctgaacg ctgaaaacta cgaaaacttc atccagaccg
    24121 atgcagcgat caaccggggt aactcaggtg gtgcgttggt taacctgaac ggcgaactga
    24181 tcggtatcaa caccgcgatc ctcgcaccgg acggcggaaa catcggtatc ggttttgcta
    24241 tcccgagcaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
    24301 gtggtgagtt gggtattatg ggcactgagc tgaactccga tctggcgaaa gcgatgaaag
    24361 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc gaattcttcc gccgcgaaag
    24421 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccaatc agcagctttg
    24481 ccgcactgcg tgctcaggtg ggcactatgc cggtaggtag caaactgacc ctgggcttac
    24541 tgcgcgatgg gaagcaggtt aacgtgaacc tggaacttca gcagagcagc cagaatcagg
    24601 ttgattccag caccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
    24661 atcagggcgt ggtggtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
    24721 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
    24781 gtaaagttct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
    24841 ccatctacct gttaatgcag taatctccct caaccccttc ctgaaaacgg gaaggggttc
    24901 tccttacaat ctgtgaactt caccacaact ccatacatct tcatcatcct ttgggcattt
    24961 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataatgg aggacttatg
    25021 gctggctggc atcttgatac caaaatggcg caggatattg tggcacgtac catgcgcatc
    25081 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
    25141 gagcgtattg gtgaattgca cgaaggagca ttgctggtac tttcacaagg acgagtcgtc
    25201 gatatcgatg acgcggtggc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
    25261 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
    25321 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
    25381 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctcattcag
    25441 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat cgatctcaat
    25501 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
    25561 atggcggagt tacagcaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
    25621 gcgattgtct cgctaaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
    25681 tgggacgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtat
    25741 ggccagttgc gttttcgcgt ttcgctgggc aactatttta ccggtcctgg cagtattgcc
    25801 cgatcctatc gtacggcgaa aacgacgatg gtagtgggga aacagcggat gccagaaagt
    25861 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
    25921 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataacggc
    25981 ttgctgcggc gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggcaacgtca
    26041 aaggcgttgt ttattcatcg taatactctg gaatatcggc ttaatcgtat atcggaactg
    26101 accgggcttg atttgggcaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
    26161 gatgaagagc ggtaggtcga ttacaaaatt acagccgggt gagactttac ccggccacag
    26221 aatggcatta tttacgcgtc agtttttcca gatcggcttc aatctcgctg atcttattgg
    26281 ttactactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga tcaacttcgc
    26341 tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga ttcgggctaa
    26401 tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt ttacgctgac
    26461 gcgggtattt aaacttcacg ctcttggcga aaaactcgcc tttgtctttc tggaaataga
    26521 ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt tcttcaggat
    26581 tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat gagtcagtaa
    26641 attgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc gcagaaagcc
    26701 tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct ttattgtggt
    26761 gatattctat gggatggatt agcacattta tcttcatttt gcgaattaca tgacagtccg
    26821 aaacaagatg aaaaagtatt taaaaaaaag ggggaaaatg agtaggacga attattctga
    26881 gccagaaagc aatcgggcag cgtcgtgctg cccgtgtaca tacttttagt cgatggtacg
    26941 cagcagttcg ttaataccaa ctttgccgcg agttttcgcg tcaactttct taacgataac
    27001 tgcacagtag aggctgtatt tgccatcttt cgacggcaga ttacctgaaa caaccacaga
    27061 ccctgccgga acgcgaccgt agtggatttc gccggtttca cggtcataaa tacgggtgct
    27121 ctgaccaatg tatacgccca tagagatgac ggaaccttct tcgacaatca ccccttcaac
    27181 cacttcagag cgtgcgccga tgaagcagtt atcttcaata atggtcgggt tagcctgcag
    27241 cggttccagc acgccgccga tacccacgcc accggaaagg tggacgtttt taccaatctg
    27301 cgcacaggaa ccgacggtcg cccaggtatc aaccatggtg ccttcatcaa catatgcgcc
    27361 gatgttgacg taagacggca tcagcacggt gttacgggca atgaacgcgc cctgacgcac
    27421 tgccgctggt ggcacgacgc ggaagccttc tttctggaaa agtgcttcat cgtagttagc
    27481 gaatttcatc ggcactttgt cgaagtagcg gctttctgcc ccttcgatca cctgattatc
    27541 attaatacgg aaagagagca gcaccgcttt tttcaaccac tgatgcgtca cccactgacc
    27601 gtcaattttt tccgctacac gcagtgcgcc ggaatccagc agggcgatca cctgatttac
    27661 cgcttcgcgg gtaacggtgt ctgcattggc tggcgtgatc tcggcacggc gttcaaaagc
    27721 ggtttcaata atgttctgta actgctgcat tgttaaactc ttttcatatc agtaaacaca
    27781 tcacccttta tcgtttggat tgagggcctc tgtcaaccgc tgatgcactt cctgctgcaa
    27841 ctcgttatta agcgcacgcc ggtcagcggt ggcaattatg aataaatctt ctactcgctc
    27901 gccaatggtt gtaattcggg cgccatgaag cgaaattccc agatcggcaa aaattttccc
    27961 gactcgcgcc agcagtccag gttggtcgag ggcgatcagt tcgaggaacg atttgcggtc
    28021 ggtatgggtc ggcaaaaacg tcacttcggt ttcaacagta aaatggcgca atttagcggg
    28081 ttggcgacgg ggctgcggtg gctgccagct actttgtgtc agtacttgct ccagaccaaa
    28141 ccgaataacc tcatgacgat ctgcggatag cgggctacca tcgggttcca gcactataaa
    28201 ggtatccatc gccataccgt cgcgagtggt gaaaatttgt gcgtcgtgaa cacttaaatt
    28261 gcggcggtct aattcggcac agacggcggc aaacagatag gggcggtccg ggctccagat
    28321 aaaaatctcg gtgcctccac gcgtagcctg cgggctaagc agtaccagcg gtttgcctaa
    28381 atcatgctgt aataaatggc gtgcgtgcca ggccagttga tttgggctat ggcggacaaa
    28441 atagttagcg cgacagcgtg accaaatttg gtgcagcgcc tcttcgtcga tgttatccat
    28501 gcgcagtagt gccagcgcct ggagttggtg atggcgaacc cgttcgcgca tatccggcgt
    28561 gttttgcatt ccgcgtcgta gctgcttttc ggtggcaaaa tagagttcgc gcaacagact
    28621 ttgcttccag ctattccaca gtgtttcgtt ggtggcgcaa atgtcagcca ctgtcaggca
    28681 taccagatag cgcagacgat tttccgtttg aacttcttcg gcaaactgct tgatgacttc
    28741 cgggtcctga atatcgcggc gctgagcggt caccgacatc agcaagtgct ggcgaaccag
    28801 ccaggcgacc agctgtgttt cgcgtgagtt cagcccgtgg agttcggcaa aatgcactac
    28861 atcctgagca ccgagaatgg agtggtcgcc gccgcgtcct ttggcgatat cgtgaaatag
    28921 cgcggcgatg aaaatcagct cagttgacgg caggcgcggc cagacgtcca cacacaacgg
    28981 atggcgctgg cgcgtttctt cactggcaaa actctctagt ttcaacatca cgcggatagt
    29041 atgttcatcc accgtgtagg cgtggaacag atcaaactgc atctgcccga cgatatgcga
    29101 ccattgcggc atatacgcgc cgagtacgct atggcgatgc attggcaata gcccgcgccg
    29161 caccgctccg gggtgacgaa gaatgctcaa aaacagtttt cgcgcttccg gaatattaca
    29221 cagaggttgt tgcagatggc gacgggcgtg gcgtaactgg cgtagcgtgg tggagtaaat
    29281 gccggtgatc gcactgttgc gcaccatggt gtagaacata cgcaagatgg cttccggctg
    29341 gcgcataaat agtgtttcat cacgcaggtc gatcagcgtt ccgcgtagct gaaactcatc
    29401 gtcgattgga cgtggttttt cgtcggcggg aagggcgagg atggcttcat cgaacagttg
    29461 cagcagcatc tggttgagtt cactgacgcg gcgtgtaacg cggaagtaat ccttcatcat
    29521 ccgctcgacc ggttcgttac cttcaccact gtaattcaaa cgctgggcga cgctaagctg
    29581 gcgatcgaat aacaggcgat tatcgtaacg actgacgacc agatgcaggg caaagcgaat
    29641 gcgccacaat atatgcaggc attcgtttaa ttccgctctc tccgcagagg ttaagaagcc
    29701 aaaccctacc atttcatcca gcgatgttgc gccaaaatga cggcgggcca cccattgcag
    29761 agtgtggata tcgcgcaagc cgccggggct acttttgatg tctggttcaa ggttgtagct
    29821 ggtgccgtgg taacgctgat ggcgctggtt ctgttcttca actttcgccg cgtagaactt
    29881 gtcggaaggc cagaatcctt cgctaaaaat atgtttttgc agttcgagga acagcgcaac
    29941 atcgccaatt aataagcggg attcgattaa attggtggcg acggttaaat ccgataaccc
    30001 ttccagcatg cactcttcaa gcgtgcgcac gctatgacct acttccagct ttacatccca
    30061 gagcagcgtt aacagctcgc ccactttttg cgcctgatcg tccgggagct ttttacggct
    30121 taaaatcagt aaatcgatgt cagaaagtgg atgcagctcg ccacgaccgt agccaccgac
    30181 ggcgaccaat gccaggtcgg caatctggct gaatcccgct tcaatccata atcgttgaag
    30241 aagctggtcg ataaactcgg tacgcgcctc aatcaactgt tctgcggaga taccattgtc
    30301 aaaggcatca cccagccaac gctggaaagt atcgatatgg gcttttatcc caccgacggt
    30361 taattcatca cggggccagg cgcatggatt ttgcggttga ccgggcaggg tggggagagc
    30421 ggtgtttgcg tactgttctg gaagggtatt cattgtgcgc cacccataag attaaattat
    30481 tgcattaaaa aagccggcat tcgccggctt cattttattc gtcgtgcgag attatcgccg
    30541 ggatggtgtc atccttgcgt agcgtcagaa tttcgcagcc gttatcagtc accacaatag
    30601 tatgctcata ttgtgcagac aagctgcgat ctttggtttt taccgtccag ccatctttca
    30661 tggtgcggat ctctttttta cccgcgttga ccattggctc gatggtgaac gtcatccccg
    30721 gtttcagcac gacgttggtt tcacgggagt catagtgcag cacctgcggt tcttcgtgga
    30781 agccgcgacc aataccgtgt ccgcaatatt cacgaacgac ggagaagcct tctgcttcga
    30841 caaatttctg aattgccgca ccgatttcgc gcagattaat gccaggtttt accatgcgta
    30901 gcgccaggta caggctttct tgcgtgatgc ggcacagacg ttcgcccatg atggtcggct
    30961 taccgacgat aaacattttc gaggtatcgc cgtggaaacc atctttgatt acggtgacat
    31021 caatgttaac gatatcgcca tctttcagca gcttggcgtc gtccgggata ccgtggcaca
    31081 ccacttcatt aatagagatg caaacggatt tcggatagcc gtgatagccg aggcaggcag
    31141 aaaccgcgtg ttgttcatta acaatgtaat cattacagat gcgatccaac tcgccggtgc
    31201 tgacgcccgg tttaacatat ggttcgatca tctccagcac ttcggcagcc agtcggccag
    31261 cgacgcgcat tttttcgata tcttctgggg tcttgattga gatagccatt aattctgtcc
    31321 atcagcgtcg gtgataccga caatatatat gtaagtgccg tcaatggtat cacacccggg
    31381 caaattgaga atcattctga atttcgccaa acgtgccact gaaggttttc tataatagaa
    31441 aattcaacgt ctgatgctgt acacagcgcc aacaattatt ggtgtccacg acgtatttgt
    31501 ggtataaagc gcgccggact tccgatccat ttcgtataca cagactggac ggaagcgaca
    31561 atctcacttt gtgtaacaac acacacgtat cggcacatat tccggggtgc cctttggggt
    31621 cggtaatatg ggatacgtgg aggcataacc ccaactttta tatagaggtt ttaatcatgg
    31681 caactgtttc catgcgcgac atgctcaagg ctggtgttca cttcggtcac cagacccgtt
    31741 actggaaccc gaaaatgaag ccgttcatct tcggtgcgcg taacaaagtt cacatcatca
    31801 accttgagaa aactgtaccg atgttcaacg aagctctggc tgaactgaac aagattgctt
    31861 ctcgcaaagg taaaatcctt ttcgttggta ctaaacgcgc tgcaagcgaa gcggtgaaag
    31921 acgctgctct gagctgcgac cagttcttcg tgaaccatcg ctggctgggc ggtatgctga
    31981 ctaactggaa aaccgttcgt cagtccatca aacgtctgaa agacctggaa actcagtctc
    32041 aggacggtac tttcgacaag ctgaccaaga aagaagcgct gatgcgcact cgtgagctgg
    32101 agaaactgga aaacagcctg ggcggtatca aagacatggg cggtctgccg gacgctctgt
    32161 ttgtaatcga tgctgaccac gaacacattg ctatcaaaga agcaaacaac ctgggtattc
    32221 cagtatttgc tatcgttgat accaactctg atccggacgg tgttgacttc gttatcccgg
    32281 gtaacgacga cgcaatccgt gctgtgaccc tgtacctggg cgctgttgct gcaaccgtac
    32341 gtgaaggccg ttctcaggat ctggcttccc aggcggaaga aagcttcgta gaagctgagt
    32401 aataaggcac gctcgtacga gcccttatag accaggttgt acacgtttgg ttagggggcc
    32461 tgcatatggc cccctttttc acttttatat ctgtgcggtt taatgccggg cagatcacat
    32521 ctccgaggat tttagaatgg ctgaaattac cgcatccctg gtaaaagagc tgcgtgagcg
    32581 tactggcgca ggcatgatgg attgcaaaaa agcactgact gaagctaacg gcgacatcga
    32641 gctggcaatc gaaaacatgc gtaagtccgg tgctattaaa gcagcgaaaa aagcaggcaa
    32701 cgttgctgct gacggcgtga tcaaaaccaa aatcgacggc aactacggca tcattctgga
    32761 agttaactgc cagactgact tcgttgcaaa agacgctggt ttccaggcgt tcgcagacaa
    32821 agttctggac gcagctgttg ctggcaaaat cactgacgtt gaagtactga aagcacagtt
    32881 cgaagaagaa cgtgttgcgc tggtagcgaa aattggtgaa aacatcaaca ttcgccgcgt
    32941 tgctgcgctg gaaggcgacg ttctgggttc ttatcagcac ggtgcgcgta tcggtgttct
    33001 ggttgctgct aaaggcgctg acgaagagct ggttaaacac atcgctatgc acgttgctgc
    33061 aagcaagcca gaattcatca aaccagaaga cgtatccgct gaagtggtag aaaaagaata
    33121 ccaggtacag ctggatatcg cgatgcagtc tggtaagccg aaagaaatcg cagagaaaat
    33181 ggttgaaggc cgcatgaaga aattcaccgg cgaagtttct ctgaccggtc agccgttcgt
    33241 tatggaacca agcaaaactg ttggtcagct gctgaaagag cataacgctg aagtgactgg
    33301 cttcatccgc ttcgaagtgg gtgaaggcat cgagaaagtt gagactgact ttgcagcaga
    33361 agttgctgcg atgtccaagc agtcttaatt atcgaaaagg agccgcctga gggcggcttc
    33421 tttttgtgcc catcttgtaa attcagctaa cccttgtggg gctgcgctga aaagcgacgt
    33481 acaatgtcgc tagtattaat tcatttcaat cgttgacagt ctcaggaaag aaacatggct
    33541 accaatgcaa aacccgtcta taaacgcatt ctgcttaagt tgagtggcga agctctgcag
    33601 ggcactgaag gcttcggtat tgatgcaagc atactggatc gcatggctca ggaaatcaaa
    33661 gaactggttg aactgggtat tcaggttggt gtggtgattg gtgggggtaa cctgttccgt
    33721 ggcgctggtc tggcgaaagc gggtatgaac cgcgttgtgg gcgaccacat ggggatgctg
    33781 gcgaccgtaa tgaacggcct ggcaatgcgt gatgcactgc accgcgccta tgtgaacgct
    33841 cgtctgatgt ccgctattcc attgaatggc gtgtgcgaca gctacagctg ggcagaagct
    33901 atcagcctgt tgcgcaacaa ccgtgtggtg atcctctccg ccggtacagg taacccgttc
    33961 tttaccaccg actcagcagc ttgcctgcgt ggtatcgaaa ttgaagccga tgtggtgctg
    34021 aaagcaacca aagttgacgg cgtgtttact gctgatccgg cgaaagatcc aaccgcaacc
    34081 atgtacgagc aactgactta cagcgaagtg ctggaaaaag agctgaaagt catggacctg
    34141 gcggccttca cgcttgctcg tgaccataaa ttaccgattc gtgttttcaa catgaacaaa
    34201 ccgggtgcgc tgcgccgtgt ggtaatgggt gaaaaagaag ggactttaat cacggaataa
    34261 ttcccgtgat ggataaataa gggtaagatt ccgcgtaagt atcgcggggg atcttacccg
    34321 taatatgttt aatcagggct atacttagca cacttccact gtgtgtgact gtctggtctg
    34381 actgagacaa gttttcaagg attcgtaacg tgattagcga tatcagaaaa gatgctgaag
    34441 tacgcatgaa caaatgcgta gaagcgttca aaacccaaat cagcaaaata cgcacgggtc
    34501 gtgcttctcc cagcctgctg gatggcattg tcgtggaata ttacggcacg ccgacgccgc
    34561 tgcgtcagct ggcaagcgta acggtagaag attcccgtac tctgaaaatc aacgtgtttg
    34621 atcgttcaat gtctccggcc gttgaaaaag cgattatggc gtccgatctt ggcctgaacc
    34681 cgaactctgc gggtagcgac atccgtgttc cgctgccgcc gctgacggaa gaacgtcgta
    34741 aagatctgac caaaatcgtt cgtggtgaag cagaacaagc gcgtgttgca gtacgtaatg
    34801 tgcgtcgtga cgcgaacgac aaagtgaaag cactgttgaa agataaagag atcagcgaag
    34861 acgacgatcg ccgttctcag gacgatgtac agaaactgac tgatgctgca atcaagaaaa
    34921 ttgaagcggc gctggcagac aaagaagcag aactgatgca gttctgattt cttgaacgac
    34981 aaaaacgccg ctcagtagat ccttgcggat cggctggcgg cgttttgctt tttattctgt
    35041 ctcaactctg gatgtttcat gaagcaactc accattctgg gctcgaccgg ctcgattggt
    35101 tgcagcacgc tggacgtggt gcgccataat cccgaacact tccgcgtagt tgcgctggtg
    35161 gcaggcaaaa atgtcactcg catggtagaa cagtgcctgg aattctctcc ccgctatgcc
    35221 gtaatggacg atgaagcgag tgcgaaactt cttaaaacga tgctacagca acaggggagc
    35281 cgcaccgaag tcttaagtgg gcaacaatcc gcttgcgata tggctgcgct tgaggatgtt
    35341 gatcaggtga tggcagccat tgttggcgct gctgggctgt tacctacgct tgctgcgatc
    35401 cgcgcgggta aaaccatttt gctggccaat aaagaatcac tggttacctg cggacgtctg
    35461 tttatggacg ccgtaaagca gagcaaagcg caattgttac cggtcgatag cgaacataac
    35521 gctatttttc agagtttacc gcaacctatc cagcataatc tgggatacgc tgaccttgag
    35581 caaaatggcg tggtgtccat tttacttacc gggtctggtg gccctttccg tgagacgcca
    35641 ttgcgcgatt tggcaacaat gacgcccgat caagcctgcc gtcatccgaa ctggtcgatg
    35701 ggacgtaaaa tttccgtcga ttcggctacc atgatgaaca aaggtctgga atacattgaa
    35761 gctcgttggc tgtttaacgc cagcgccagc cagatggaag tgctgattca cccgcagtca
    35821 gtgattcact caatggtgcg ctaccaggac ggcagtgttc tggcgcagct gggtgaaccg
    35881 gatatgcgta cgccaattgc tcacaccatg gcatggccga atcgcgtgaa ctctggcgtg
    35941 aagccgctcg atttttgcaa actaagtgcg ttgacatttg ccgcaccgga ttatgatcgt
    36001 tatccatgtc tgaaactggc gatggaggcg tttgaacaag gccaggcagc gacgacagca
    36061 ttgaatgccg caaacgaaat caccgtggct gcttttcttg cgcaacaaat ccgctttacg
    36121 gatatcgctg cgttgaattt atccgtactg gaaaaaatgg atatgcgcga accacaatgt
    36181 gtggatgatg tgttatctgt tgatgcgaac gcgcgtgaag tcgccagaaa agaggtgatg
    36241 cgtctcgcaa gctgaggata atccggctac agagagtcgc gctatttgtt agcgtagggc
    36301 ttcagtgata tagtctgcgc catctgatcg taagtagttg gctttataag gtcaggtatg
    36361 ccgtggtttt acacggcttt tttttgtata ggcttcagta ttcctgagta ccgtaaaccc
    36421 tgtcagggaa taagaaacgc gtgatgttgt ctgctactca accacttagc gaaaaattgc
    36481 cagcgcatgg ctgtcgtcat gttgcgatca ttatggacgg caatggtcgc tgggcaaaaa
    36541 agcaagggaa gattcgtgcc tttgggcata aagccggggc aaaatccgtc cgccgggctg
    36601 tctcttttgc ggccaacaac ggtattgagg cgttaacgct gtatgccttt agtagtgaaa
    36661 actggaaccg accagcgcag gaagtcagtg cgttaatgga actgtttgtg tgggcgctag
    36721 atagcgaagt aaaaagtctg caccgacata acgtgcgtct gcgtattatt ggcgatacca
    36781 gtcgctttaa ctcgcgtttg caagaacgta ttcgtaaatc tgaagcgcta acagccggga
    36841 ataccggtct gacgctgaat attgcggcga actacggtgg acgttgggat atagtccagg
    36901 gagtcaggca actggctgaa aaggtgcagc aaggaaacct gcaaccagat cagatagatg
    36961 aagagatgct aaaccagcat gtctgtatgc atgaactggc ccctgtagat ttagtaatta
    37021 ggactggggg ggagcatcgc attagtaact ttttgctttg gcaaattgcc tatgccgaac
    37081 tttactttac agatgttctc tggcccgatt tcgatgaaca agactttgaa ggggcgttaa
    37141 atgcctttgc taatcgagag cgtcgtttcg gcggcaccga gcccggtgat gaaacagcct
    37201 gatgggggtc gcttttgctg aagtatcgcc tgatatctgc ttttgtgtta atacccgtcg
    37261 tcatcgcggc gttgtttctg ttgccgccgg tggggttcgc cattgtaacg ctggtggtct
    37321 gtatgctggc agcgtgggaa tggggacagc ttagcggttt taccactcgt tcgcagcgag
    37381 tatggttggc ggtgttatgt gggttattgt tggcgctgat gctttttctg ttgccggaat
    37441 atcaccgaaa tattcatcaa ccgctggttg aaatctcact ttgggcttcg ctgggttggt
    37501 ggattgtcgc gctattgctg gtgctgtttt acccaggttc cgcagcaatc tggcgtaact
    37561 ctaaaacatt gcgcattatt tttggcgtgc taaccattgt tcccttcttc tggggcatgc
    37621 tggcgttacg ggcctggcac tacgacgaga atcattacag tggcgcaata tggctgctct
    37681 atgtcatgat cctggtatgg ggcgctgact ctggcgcata tatgtttggc aaattgtttg
    37741 gtaaacataa actggcaccg aaggtttctc cgggtaaaac ctggcaaggc tttatcggtg
    37801 gactagtgac cgcagcggta atctcatggg gttatggcat gtgggtgaat ctcgacgtcg
    37861 ctcccgtcac cttactcatt tgctctattg tcgcagcgtt agcctcagtg ctcggcgatc
    37921 tgaccgaaag tatgtttaag cgtgaagcag gaattaagga cagcggtcat ttaattccag
    37981 gacacggtgg tattttagat cgtattgata gcctgacggc tgcggtaccg gtctttgctt
    38041 gcttgttgtt actggtattc aggacgcttt aacggaaggt aatatgctga gttttctctg
    38101 ggatttggct tcgttcatcg ttgcactggg tgtacttatc accgtgcatg aatttggtca
    38161 tttctgggtt gcccggcgtt gtggtgttcg cgttgagcgt ttctcaatag ggtttggtaa
    38221 ggcgctctgg cggcgaactg ataagctcgg caccgaatat gttatcgccc tgatcccgtt
    38281 gggcggttat gtcaaaatgc tggatgagcg cgcagaaccg gtcgttccgg aactccgcca
    38341 ccatgccttc aataataaat ctgtcggcca acgagcggcg attattgccg caggtccggt
    38401 tgcaaacttc atttttgcta tctttgccta ctggctggtt tttattattg gtgtgcctgg
    38461 cgtacgtccg gtggttggtg aaatagcagc caattcgata gctgcggaag cacaaattgc
    38521 accaggtacg gaactaaaag ccgtagatgg tatcgaaacg cctgattggg atgccgtgcg
    38581 tttgcagttg gtcgataaaa ttggcgatga aagcaccacc attacggtag cgccatttgg
    38641 cagcgaccaa cggcgggatg taaagctcga tttacgtcac tgggcgtttg agcctgataa
    38701 agaagatccg gtatcttcgt tggggattcg tcctcgtggg ccgcaaattg aacctgtact
    38761 ggaaaatgtg cagccaaact cggcggcaag caaggcaggt ttgcaagcag gcgacaggat
    38821 cgttaaagtc gatggtcagc ccttaacgca gtgggtgacc tttgtgatgc ttgtccggga
    38881 taacccgggt aaatccttag cgttagaaat cgaaaggcag gggagtccct tgtctttgac
    38941 attaatcccg gagagtaaac cgggtaaggg taaagcgatt ggttttgtcg gtattgagcc
    39001 gaaagtcatt cctttgccag atgagtataa agttgtacgc cagtatgggc cgttcaacgc
    39061 tatcgtcgaa gccacggaca aaacgtggca gttgatgaag ctgacggtca gtatgctggg
    39121 aaaattgatc accggtgatg tgaaactgaa caacctcagt gggccgatct ctatcgccaa
    39181 gggggctggg atgacagcgg aactcggggt agtttattac ctgccgtttc ttgcgcttat
    39241 tagcgtgaac ttagggataa ttaacctgtt tccgttgccc gtacttgacg gggggcatct
    39301 gctgttcctt gcgatcgaaa agatcaaggg cggaccggta tccgagcggg ttcaagactt
    39361 ttgttatcgc attggctcga ttctgctggt gctgttaatg gggcttgcac ttttcaatga
    39421 tttctctcgg ttatgagagt tagttaggaa gaacgcataa taacgatggc gatgaaaaag
    39481 ttgctcatag cgtcgctgct gtttagcagc gccaccgtat acggtgctga agggttcgta
    39541 gtgaaagata ttcatttcga aggccttcag cgtgtcgccg ttggtgcggc cctcctcagt
    39601 atgccggtgc gcacaggcga cacggttaat gatgaagata tcagtaatac cattcgcgct
    39661 ctgtttgcta ccggcaactt tgaggatgtt cgcgtccttc gtgatggtga tacccttctg
    39721 gttcaggtaa aagaacgtcc gaccattgcc agcattactt tctccggtaa caaatcggtg
    39781 aaagatgaca tgctgaagca aaacctcgag gcttctggcg tgcgtgtggg cgaatccctc
    39841 gatcgcacca ccattgccga tatcgagaaa ggtctggaag acttctacta cagcgtcggt
    39901 aaatatagcg ccagcgttaa agctgtcgtg accccgctgc cgcgcaaccg tgttgaccta
    39961 aaactggtgt tccaggaagg tgtgtcagct gaaatccagc aaattaacat tgttggtaac
    40021 catgctttca ccaccgacga actgatctct catttccaac tgcgtgacga agtgccgtgg
    40081 tggaacgtgg taggcgatcg taaataccag aaacagaaac tggcaggcga ccttgaaacc
    40141 ctgcgcagtt actatctgga tcgcggttat gcccgtttca acatcgactc tacccaggtc
    40201 agtctgacgc cagataaaaa aggtatttac gtcacggtga acatcaccga aggcgatcag
    40261 tacaagcttt ctggcgttga agttagcggc aatcttgccg ggcactccgc tgaaattgag
    40321 caactgacta agatcgagcc gggcgagctg tataacggca ccaaagtgac caaaatggaa
    40381 gatgacatta aaaagcttct tggtcgctat ggttatgcct atccgcgcgt acagtcgatg
    40441 cctgaaatta acgatgccga caaaaccgtt aaattacgcg tgaacgttga tgcgggtaac
    40501 cgtttctatg tgcgtaagat ccgttttgaa ggtaacgata cctcgaaaga tgccgtcctg
    40561 cgtcgcgaaa tgcgtcagat ggaaggtgca tggctgggga gcgatctggt cgatcagggt
    40621 aaggagcgtc tgaaccgtct gggcttcttt gaaactgtcg ataccgatac ccaacgtgtt
    40681 ccggggagcc cggatcaggt tgatgtagtt tacaaggtga aagaacgtaa cactggtagc
    40741 ttcaacttcg ggattggtta cggtactgaa agtggcgtga gcttccaggc tggcgtgcaa
    40801 caggataact ggttaggtac aggttatgct gttggtatca acgggaccaa aaacgattac
    40861 cagacctatg ctgaactgtc ggtaaccaac ccgtacttca ccgtagatgg tgtaagcctc
    40921 ggtggtcgtc tcttctataa tgacttccag gcagatgacg ccgacctgtc cgactatacc
    40981 aacaagagtt atggtacaga cgtgacgttg ggcttcccga tcaacgaata taactcgctg
    41041 cgtgcaggtc tgggttatgt acataactcc ctgtccaaca tgcagcctca ggttgcgatg
    41101 tggcgttatc tgtactctat gggtgaacat ccgagcacct ctgatcagga taacagcttc
    41161 aaaacggacg acttcacgtt caactatggt tggacctata acaagcttga ccgtggttac
    41221 ttcccgacag atggttcacg tgtcaacctg accggtaaag tgaccattcc gggatcggat
    41281 aacgaatact acaaagtgac gttagacacg gcgacttatg tgccgattga tgacgatcac
    41341 aaatgggttg ttctgggtcg tacccgctgg ggttatggtg atggtttagg cggcaaagag
    41401 atgccgttct atgagaactt ctatgccggt ggttccagca ccgtgcgtgg cttccagtcc
    41461 aataccattg gtccgaaagc agtttacttc ccgcatcagg ccagtaatta tgatccggac
    41521 tatgattacg aatgtgcgac tcaggacggc gcgaaagatc tgtgtaaatc ggatgatgct
    41581 gtaggcggta acgccatggc ggttgccagc ctcgagttca tcaccccgac gccgtttatt
    41641 agcgataagt atgctaactc ggttcgtact tccttcttct gggatatggg taccgtttgg
    41701 gatacaaact gggattccag ccaatattct ggatatccgg actatagtga tccaagcaat
    41761 atccgtatgt ctgcgggtat cgcattacaa tggatgtccc cattggggcc gttggtgttc
    41821 tcctacgccc agccgttcaa aaagtacgat ggagacaagg cagaacagtt ccagtttaac
    41881 atcggtaaaa cctggtaagt gttctccaca aaggaatgta gtggtagtgt agcgatgact
    41941 ttaggcgatc aatataagat cgccgggcca cgcaaagaac tgcaccctcc ggtgcaaatg
    42001 ggatggtaag gagtttattg tgaaaaagtg gttattagct gcaggtctcg gtttagcact
    42061 ggcaacttct gctcaggcgg ctgacaaaat tgcaatcgtc aatatgggca gcctgttcca
    42121 gcaggtagcg cagaaaaccg gtgtttctaa cacgctggaa aacgagttca aaggccgtgc
    42181 cagcgaactg cagcgtatgg aaaccgatct gcaggctaaa atgaaaaagc tgcagtccat
    42241 gaaagcgggc agcgatcgca ctaagctgga aaaagacgtg atggctcagc gccagacttt
    42301 tgctcagaaa gcgcaggctt ttgagcagga tcgcgcacgt cgttccaacg aagaacgcgg
    42361 caaactggtt actcgtatcc agactgctgt gaaatccgtt gccaacagcc aggatatcga
    42421 tctggttgtt gatgcaaacg ccgttgctta caacagcagc gatgtaaaag acatcactgc
    42481 cgacgtactg aaacaggtta aataagtaat gccttcaatt cgactggctg atttagcgca
    42541 gcagttggat gcagaactac acggtgatgg cgatatcgtc atcaccggcg ttgcgtccat
    42601 gcaatctgca caaacaggtc acattacgtt catggttaac ccaaaatacc gtgagcattt
    42661 aggcttgtgc caggcgtccg cggttgtcat gacccaggac gatcttcctt tcgcgaaaag
    42721 tgccgcgctg gtagtgaaga atccctacct gacttacgcg cgcatggcgc aaattttaga
    42781 taccacgccg cagcccgcgc agaagattgc acccagtgcg gtgattgacg cgacggcgaa
    42841 gctgggtaac aacgtatcga ttggcgctaa cgcggtgatt gagtccggcg ttgaactggg
    42901 cgataacgtg attatcggtg ccggttgctt cgtaggtaaa aacagcaaaa tcggtgcagg
    42961 ttcgcgtctc tgggcgaacg taaccattta ccatgagatc cagatcggtc agaattgcct
    43021 gatccagtcc ggaacagtgg taggcgcaga cggctttggt tatgccaacg atcgtggtaa
    43081 ctgggtgaag atcccacaga ttggtcgggt aattattggc gatcgcgtgg agatcggtgc
    43141 ctgcactact attgatcgtg gcgcgctgga tgacactgtt attggcaatg gtgtgatcat
    43201 tgataaccag tgccagattg cacataacgt cgtgattggc gacaatacgg cagttgccgg
    43261 tggcgtcatt atggcgggca gcctgaaaat tggtcgttac tgcatgatcg gcggagccag
    43321 cgtaatcaac gggcatatgg aaatatgcga caaagtgacg gttacgggca tgggtatggt
    43381 gatgcgtccc atcactgaac caggcgtcta ttcctcaggc attccgctgc aacccaacaa
    43441 agtctggcgc aaaaccgctg cactggtgat gaacattgat gacatgagca agcgtctgaa
    43501 atcgcttgag cgcaaggtta atcaacaaga ctaacgttcc atcttttgtt cgccaaactt
    43561 tacggcctgt ctcattctta cgattgcggc aggccgtgtt attattgtcg tttcttatat
    43621 tttgacagga agagtatctt gactactaac actcatactc tgcagattga agagatttta
    43681 gaacttctgc cgcaccgttt cccgttctta ctggtggatc gcgtgctgga ttttgaagaa
    43741 ggtcgttttc tgcgcgcagt aaaaaatgtc tctgtcaatg agccattctt ccagggccat
    43801 ttccctggaa aaccgatttt cccgggtgtg ctgattctgg aagcaatggc acaggcaaca
    43861 ggtattctgg cgtttaaaag cgtaggaaaa ctggaaccgg gtgagctgta ctatttcgct
    43921 ggtattgacg aagcgcgctt caagcgcccg gtcgtgcctg gcgatcaaat gatcatggaa
    43981 gtcactttcg aaaaaacgcg ccgcggcctg acccgtttta aaggggttgc tctggtcgac
    44041 ggtaaagtag tttgcgaagc aacgatgatg tgtgctcgta gccgggaggc ctgatacgtg
    44101 attgataaat ccgcctttgt gcatccaacc gccattgtgg aagagggcgc gtcgattggc
    44161 gctaacgctc acattggtcc tttttgtatc gttggacccc atgtcgaaat tggtgagggt
    44221 accgtactga aatctcacgt tgtcgtgaat ggccatacta aaattggccg cgataatgag
    44281 atttatcagt tcgcctccat cggcgaagtt aaccaggatc tgaaatatgc tggcgaaccg
    44341 acccgcgtgg aaatcggcga tcgtaaccgc attcgcgaaa gcgtcaccat tcatcgtggc
    44401 acagtccagg gcggtggatt gacgaaggtg ggcagcgaca acttactgat gatcaacgcg
    44461 cacattgcgc acgattgtac ggtaggtaac cgctgtattc tcgccaacaa cgcaacgctg
    44521 gcgggtcacg tatcggttga cgacttcgcg atcatcggcg gcatgaccgc agtccatcag
    44581 ttctgcatca ttggtgcgca tgtgatggtt ggcggctgct ccggtgtggc gcaggacgtc
    44641 cctccttatg tcattgcgca gggtaaccac gcaacgccgt tcggtgtcaa tatcgaaggg
    44701 ctgaagcgcc gcggattcag ccgtgaggcg attaccgcta tccgcaatgc gtataagctg
    44761 atttatcgta gcggtaaaac gctcgatgaa gtgaaaccgg aaattgctga actggcggaa
    44821 acatatccgg aagtgaaagc ctttaccgat ttctttgcac gctcaacgcg cggtctgatt
    44881 cgttaatgac tgaacagcgt ccattaacga ttgccctggt cgccggagaa acctccggcg
    44941 atatcctggg ggccggttta atccgcgctc tgaaagaacg tgtgcccaac gcccgctttg
    45001 ttggtgttgc cgggccacga atgcaggctg aaggctgcga agcctggtac gaaatggaag
    45061 aactggcagt gatgggcatt gttgaagtgc tcggtcgtct gcgtcgctta ctacatattc
    45121 gtgccgatct gacaaagcgt tttggcgaac tgaagccaga tgtttttgtt ggtattgatg
    45181 cgcctgactt caatattact cttgaaggta acctcaaaaa gcagggtatc aaaaccattc
    45241 attacgtcag tccgtccgta tgggcgtggc gacagaaacg cgttttcaaa ataggcagag
    45301 ccaccgatct ggtgctcgca tttctgcctt tcgaaaaggc gttttatgac aaatacaacg
    45361 taccgtgccg ctttatcggt cataccatgg ctgatgccat gccattagat ccagataaaa
    45421 atggtgcccg tgatgtgctg gggatccctt acgatgccca ctgtctggca ttgttgccgg
    45481 gcagccgtgg tgcagaagtc gaaatgctta gtgccgattt cttgaaaacg gcccagcttt
    45541 tgcgccagac atatccggat ctcgaaatcg tggtgccgct ggtgaatgcc aaacgccgcg
    45601 agcagtttga acgcatcaaa gctgcagtcg cgccagacct gtcggttcat ttgctggatg
    45661 gaatgggccg tgaggcgatg gtcgccagtg atgcggcgct actggcgtcg ggtacggcag
    45721 cactggagtg tatgctggct aaatgcccga tggtagtggg atatcgcatg aagcctttca
    45781 ccttctggct ggcgaagcga ctggtgaaaa ctgattatgt ctcgctgcca aatctgctgg
    45841 cgggcagaga gttagtcaaa gagctattgc aggaagagtg tgagccgcaa aaattggctg
    45901 cggcgctgtt accgctgttg gcgaacggga aaaccagcca tgcgatgcac gacaccttcc
    45961 gcgagctgca tcagcagatc cgctgcaatg ccgatgagca ggcggcacaa gccgttctgg
    46021 agttagcaca atgatcgaat ttgtctatcc gcacacgcag ctggttgcgg gtgtggatga
    46081 agtcggacgc gggccgttag ttggcgcggt cgtcaccgct gcggtgatcc ttgacccggc
    46141 gcgcccaatt gccgggctga atgattccaa aaagctgagc gaaaaacgcc gtctggcgct
    46201 ctgtgaagag atcaaagaga aagcgttgag ctggagtctg ggccgcgcgg aacctcacga
    46261 aatcgacgaa ctgaatattt tacatgcgac catgctggcg atgcagcgtg ccgtcgctgg
    46321 actgcatatt gcgccggaat atgtgttgat tgatggtaac cgctgcccga aattaccgat
    46381 gccttcgatg gctgtggtga aaggcgataa ccgcgtaccg gaaatcagtg ccgcgtctat
    46441 cctggcgaaa gtgacgcgtg acgccgaaat ggcagcgctg gatattgttt tcccgcaata
    46501 tggttttgcc caacacaaag ggtatccaac cgcttttcat ctggaaaaac tggctgaaca
    46561 cggcgcgacc gaacaccatc ggcgcagctt tgggcctgtc aaacgcgcac tgggacttgc
    46621 gtcctgattc ttgtgtcgag attaagtaaa ccggaatctg aagatgtctg aaccacgttt
    46681 cgtacacctg cgggtgcaca gcgactactc gatgatcgat ggcctggcca aaaccgcacc
    46741 gctggtaaaa aaggcggcag cgttgggtat gccagcactg gcgatcaccg atttcaccaa
    46801 cctttgcggt ctggtgaagt tctacggagc gggacatggc gcagggatta agcccatcgt
    46861 cggggcggat tttaacgtcc agtgcgacct gctgggtgat gagttaactc acctgacggt
    46921 gctggcggcg aacaataccg gctatcagaa tctgacgttg ctgatctcaa aagcgtatca
    46981 gcgcgggtac ggtaccgccg ggccgatcat cgatcgcgac tggcttatcg aattaaatga
    47041 agggttgatc cttctttccg gcgggcgcat gggcgacgtt ggacgcagtc ttttgcgtgg
    47101 taacagcgcg ctggtagatg agtgtgtcgc gttttatgaa gaacacttcc cggatcgcta
    47161 ttttctcgag ctgatccgca ccggcaggcc ggacgaagaa agctatctgc acgctgcggt
    47221 ggaactggcg gaagcacgcg gtttgcccgt tgtggcgacc aacgacgtgc gctttatcga
    47281 cagcagcgac tttgacgcac acgaaatccg cgtcgcgatc cacgacggct ttaccctcga
    47341 cgatcctaaa cgcccgcgta actattcgcc gcagcaatat atgcgtagcg aagaggagat
    47401 gtgtgagctg tttgccgaca tccccgaagc ccttgccaac accgttgaga tcgccaaacg
    47461 ctgtaacgtg accgtgcgcc ttggtgaata cttcctgccg cagttcccga ccggggacat
    47521 gagcaccgaa gattatctgg tcaagcgtgc aaaagagggc ctggaagagc gtctggcatt
    47581 tttattccct gacgaggaag aacgtcttaa gcgtcgtccg gagtatgacg aacgtctgga
    47641 tactgaactt caggttatca accagatggg cttcccgggt tacttcctca tcgttatgga
    47701 atttatccag tggtcgaaag ataacggtgt accggtaggg ccaggccgtg gttctggtgc
    47761 agggtcactg gtggcctacg cgttaaaaat caccgatctc gatccgctgg aatttgacct
    47821 gctgttcgaa cgtttcctta acccggaacg tgtctccatg cctgacttcg acgttgactt
    47881 ctgtatggag aaacgcgacc aggttatcga gcacgtagcg gacatgtacg gtcgcgatgc
    47941 ggtatcgcag attatcacct tcggtacgat ggcggcgaaa gcggtgatcc gcgacgtagg
    48001 ccgtgtattg gggcatccgt acggctttgt cgaccgtatc tcgaaactga tcccgcccga
    48061 tccgggtatg acgctggcga aagcgtttga agccgagcca cagctgccgg aaatctacga
    48121 agcggatgaa gaagttaagg cgctgatcga catggcgcgc aaactggaag gggtcacccg
    48181 taacgccggt aagcacgccg gtggggtggt tatcgcgccg accaaaatta ctgattttgc
    48241 gccgctttat tgcgatgaag agggcaaaca tccggtcacc cagtttgata aaagcgacgt
    48301 tgaatacgcc gggctggtga agttcgactt cctcggtttg cgtacgctca ccatcatcaa
    48361 ctgggcgctg gagatgatca acaagcggcg ggcgaagaat ggcgagccgc cgctggatat
    48421 cgccgcgatc ccgctggatg acaagaaaag cttcgacatg ctgcaacgct cggaaaccac
    48481 ggcggtattc cagcttgaat cgcgcggcat gaaggacctg atcaagcgtc tgcaacctga
    48541 ctgcttcgaa gatatgatcg cactggtggc actgttccgc cccggcccgt tgcaatcagg
    48601 gatggtggat aactttatcg accgtaaaca tggtcgcgaa gagatctcct atccggacgt
    48661 gcagtggcag catgaaagcc tgaaaccggt actggagcca acctacggca tcatcctgta
    48721 tcaggaacag gtcatgcaga ttgcccaggt gctttctggt tataccctcg gtggcgcgga
    48781 tatgctgcgt cgtgcgatgg gtaagaaaaa gccggaagag atggctaagc agcgttctgt
    48841 atttgctgaa ggtgcagaaa agaacggaat caacgccgaa ctggcgatga aaatcttcga
    48901 cctggtggag aaattcgcgg gttacggatt taacaaatcg cactctgcgg cctatgcttt
    48961 ggtgtcatat caaacgttat ggctgaaagc gcactatccg gcggagttta tggcggcggt
    49021 aatgaccgcc gatatggaca acaccgagaa ggtggtgggc ctggtggatg agtgctggcg
    49081 gatggggctg aaaatcctgc caccagatat aaactccggt ctttaccatt tccacgtcaa
    49141 cgacgacggc gaaatcgtgt atggtattgg cgcgatcaaa ggggtaggtg aaggcccgat
    49201 tgaggccatc atcgaagccc gtaataaagg cggctacttc cgcgaactgt ttgatctctg
    49261 cgcccgtacc gacaccaaaa agttaaaccg gcgggtgctg gaaaaactga tcatgtccgg
    49321 ggcgtttgac cgtcttgggc cacaccgcgc ggcgctgatg aactcgctgg gtgatgcgtt
    49381 aaaagcggca gaccaacacg cgaaagcgga agctatcggt caggccgata tgttcggcgt
    49441 gctggccgaa gagccggaac aaattgaaca atcctacgcc agctgccaac cgtggccgga
    49501 gcaggtggta ttagatgggg aacgtgaaac gttaggtctg tacctgacgg gacaccctat
    49561 caaccagtat ttaaaagaga ttgagcgtta tgtcggaggc gtaaggctga aagacatgca
    49621 cccgacagaa cgtggtaaag tcatcacggc tgcggggctc gttgttgccg cgcgggttat
    49681 ggtcaccaag cgcggcaatc gtatcggtat ctgcacgctg gatgaccgtt ccgggcggct
    49741 ggaagtgatg ttgtttactg acgccctgga taaataccag caattgctgg aaaaagaccg
    49801 catacttatc gtcagcggac aggtcagctt tgatgacttc agcggtgggc ttaaaatgac
    49861 cgctcgcgaa gtgatggata ttgacgaagc ccgggaaaaa tatgctcgcg ggcttgctat
    49921 ctcgctgacg gacaggcaaa ttgatgacca gcttttaaac cgactccgtc agtctctgga
    49981 accccaccgc tctgggacaa ttccagtaca tctctactat cagagggcgg atgcacgcgc
    50041 gcgattgcgt tttggcgcga cgtggcgtgt ctctccgagc gatcgtttat taaacgatct
    50101 ccgtggcctc attggttcgg agcaggtgga actggagttt gactaataca ggaatactat
    50161 gagtctgaat ttccttgatt ttgaacagcc gattgcagag ctggaagcga aaatcgattc
    50221 tctgaccgcg gttagccgtc aggatgagaa actggatatt aacatcgatg aagaagtgca
    50281 tcgtctgcgt gaaaaaagcg tagaactgac gcgtaaaatc ttcgccgatc tcggtgcatg
    50341 gcagattgcg caactggcac gccatccaca gcgtccttat accctggatt acgttcgcct
    50401 ggcatttgat gaatttgacg aactggctgg cgaccgcgcg tatgcagacg ataaagctat
    50461 cgtcggtggt atcgcccgtc tcgatggtcg tccggtgatg atcattggtc atcaaaaagg
    50521 tcgtgaaacc aaagaaaaaa ttcgccgtaa ctttggtatg ccagcgccag aaggttaccg
    50581 caaagcactg cgtctgatgc aaatggctga acgttttaag atgcctatca tcacctttat
    50641 cgacaccccg ggggcttatc cgggcgtggg cgcagaagaa cgtggtcagt cggaagccat
    50701 tgcacgcaac ctgcgtgaaa tgtctcgcct cggcgtaccg gtagtttgta cggttatcgg
    50761 tgaaggtggt tctggtggtg cgctggcgat tggcgtgggc gataaagtga atatgctgca
    50821 atacagcacc tattccgtta tctcgccgga aggttgtgcg tccattctgt ggaagagcgc
    50881 tgacaaagcg ccgctggcgg ctgaagcgat gggtatcatc gctccacgtc tgaaagagct
    50941 gaaactgatc gattccatca tcccggaacc gctgggcggt gctcaccgta acccggaagc
    51001 gatggcggca tcgttgaaag cgcaactgct ggcggatctg gccgatctcg acgtgttaag
    51061 cactgaagat ttaaaaaatc gtcgttatca gcgcctgatg agctacggct acgcgtaatc
    51121 ggcaaatgtt ctgaaaaggg tcacttcggt ggcccttttt tatcgccacg gtttgagcag
    51181 gctatgatta aggaaggatt ttccaggagg aacacatgaa catcattgcc attatgggac
    51241 cgcatggcgt cttttataaa gatgagccca tcaaagaact ggagtcggcg ctggtggcgc
    51301 aaggctttca gattatctgg ccacaaaaca gcgttgattt gctgaagttt atcgaacata
    51361 acccacgaat ttgcggcgtg atttttgact gggatgagta cagtctcgat ttatgtagcg
    51421 atatcaatca acttaatgaa tatctcccgc tttatgcctt catcaacacc cactcgacga
    51481 tggatgtcag cgtgcaggat atgcggatgg cgctctggtt ttttgaatat gcgctggggc
    51541 aggcggaaga tatcgccatt cgtatgcgtc agtataccaa cgaatatctt gataacatta
    51601 cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga gcggaagtac accttttgta
    51661 cgccggggca tatgggcggc accgcatatc aaaaaagccc ggttggctgt ctgttttatg
    51721 attttttcgg cgggaatacc ctcaaggcgg acgtctctat ttcggtcacc gagcttggtt
    51781 cgttgctcga ccacaccggg ccacacctgg aagcggaaga gtacatcgcg cggacgtttg
    51841 gcgcggaaca gagttatatc gttaccaacg gaacatcgac gtcgaacaaa attgtgggta
    51901 tgtacgccgc gccatccggc agtacgctgt tgatcgaccg caattgtcat aaatcgctgg
    51961 cgcatctgtt gatgatgaac gatgtagtgc cagtctggct gaaaccgacg cgtaatgcgt
    52021 tggggattct gggtgggatc ccgcgcggtg aatttactcg cgacagcatc gaagagaaag
    52081 tcgctgccac cacgcaggca caatggccgg ttcatgcggt gatcaccaac tccacctatg
    52141 atggcttgct ctacaacacc gactggatca aacagacgct ggatgtcccg tcgattcact
    52201 tcgattctgc ctgggtgccg tacacccatt ttcatccaat ctaccagggt aaaagtggta
    52261 tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac gcagtcgacc cacaaaatgc
    52321 tggcggcgtt atcgcaggcg tcgctgatcc acattaaagg tgagtatgac gaagaggcgt
    52381 ttaacgaagc ctttatgatg cataccacca cctcgcccag ttatcccatt gttgcttctg
    52441 ttgagacggc ggcggcgatg ttgcgtggta atccgggcaa acggctgatt aaccgttcag
    52501 tagaacgagc tctgcatttt cgcaaagagg tccagcggct gcgggaagag tctgacggct
    52561 ggtttttcga tatctggcaa ccgccgcagg tggatgaagc cgaatgctgg cccgttgcgc
    52621 ctggcgaaca gtggcacggc tttagcgatg cggatgccaa tcacatgttt ctcgatccgg
    52681 ttaaagtcac tattttgaca ccggggatgg acgagcaggg caatatgagc gaggagggga
    52741 tcccggcggc gctggtggca aaattcctcg acgaacgtgg gatcgtagta gagaaaaccg
    52801 gcccttataa cctgctgttt ctctttagta ttggcatcga taaaaccaaa gcaatgggat
    52861 tattgcgtgg gttgacggaa tttaagcgct cttacgatct caacctgcgg atcaaaaata
    52921 tgctgcccga tctctatgca gaagatcccg atttctaccg caatatgcgt attcaggatc
    52981 tggcgcaagg gatccataag ctgattcgta aacacgatct tcccggtttg atgttgcggg
    53041 catttgatac cttgccggag atgatcatga cgccacatca ggcatggcag agacagatta
    53101 aaggcgaagt agaaaccatt gcgctggaac aactggtcgg tagagtatcg gcaaatatga
    53161 tcctgcctta tccacccggc gtaccgctgc tgatgccggg agaaatgctt accgaagaga
    53221 gccgcacggt actcgatttt ctactgatgc tttgttccgt agggcaacat taccccggtt
    53281 ttgaaacgga tattcacggc gcgaaacagg acgaagacgg tgtttaccgc gtacgagtcc
    53341 taaaaatggc aggataactt gccagagcgg cttccgggcg agtaacgtgc tgttaacaaa
    53401 taaaggagac gttatgctgg gtttaaaaca ggttcaccat attgcgatta ttgcgacgga
    53461 ttatgcgatg agcaaagctt tctactgcga tattcttggt ttcacgctgc aaagcgaagt
    53521 ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg cttaatgggc aatatgtgat
    53581 tgagcttttc tcatttccgt tcccgccgga gcgccccagc cgaccggaag cttgcggcct
    53641 gcgtcatctg gcttttagcg ttgatgatat cgatgcggca gtggcgcacc ttgaaagcca
    53701 taacgtgaag tgtgaagcca tccgtgtcga tccctacacg caaaaacgct tcacgttctt
    53761 taacgatccg gacgggctgc cgttggaact gtatgagcag taaggcttgt catcgccgca
    53821 tttgcccggt aacgtgcccg gcattgctac tgtaaaatcg caccatcatg acactcacgc
    53881 tcaatagaca acttctctcc tcacgccaga ttctggtggc ctttagcggc ggccttgatt
    53941 ctactgtctt actgcatcaa ctggtgcagt ggcggacgga aaatccgggc gtcactctgc
    54001 gcgctatcca tgtgcatcac ggtttaagcg ccaatgctga tgcctgggtg aaacattgtg
    54061 aaaacatctg ccaacagtgg caggtgccgc tggtggtcga acgtgtgcag cttgcgcaag
    54121 aggggctagg tattgaggcc caggcgcggc aggcacgtta tcaggcattt gcccgcacct
    54181 tgttgcccgg tgaagtgctg gttacagcgc aacatctcga cgatcaatgt gaaacctttc
    54241 tgctggcgct aaaacgcggt agcggccctg ccgggctttc ggctatggcg gaagtttcgg
    54301 agtttgccgg aacacagctt atccgcccgt tgcttgcccg cacgcggggg gaactggtgc
    54361 agtgggcgtt ggcgcatggt ttacgctgga ttgaagacga aagtaatcag gatgatagct
    54421 acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt gcagcagcgt tggccgcatt
    54481 ttgccgaagc aacagcccgc agcgccgcac tttgcgctga acaagagagc ctgttagatg
    54541 aactgctggc ggatgattta gcgcactgtc aaactccgca ggggacgctg cagattgcgc
    54601 caatgctggc gatgagtgat gcccgtcgcg cggcgattat ccgccgctgg ctggcagggc
    54661 agaatgcacc gatgccttcc cgcgacgcgt tggtgaggat ctggcaggaa gtggcgctgg
    54721 cgcgggaaga tgcctccccc tgtttacgtt tgggcgcgtt tgaaatccgc cgctatcagt
    54781 cgcaactatg gtggattaaa tccgttgccg ggcaaagcga aaccattgtg ccgtggcaga
    54841 cgtggctaca accgctggaa ttaccggcgg ggctgggaag tgtacagctt actgcgggag
    54901 gcgatattcg ccctccgcgt gcagatgaag cggtcagcgt gcgtttcaaa gcgccaggac
    54961 tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa gaaaatctgg caagagttag
    55021 gcgttccgcc gtggctacgt gataccacgc cactgctgtt ttatggcgaa acgctgattg
    55081 cggcggcagg ggtatttgtg acgcaagaag gtgtggctgt aggtgagaat ggcgtcagtt
    55141 ttgtctggca gaaaacgctt agttaagtga aagccggata agacgcttca aacgtcgcat
    55201 ccggcgaaag tcaatcagga ctcgctcacc accaccgtac cgatttccgg gtggctaaag
    55261 ctggtaattt tatccagacg cagctcgcgg gttgcaccag cggcttcgac gaccagatat
    55321 tccacatttt tgcgggagac taaatcgctg gccttcgcct gcaatttttc gccatctttc
    55381 agctcaagtg ccagcattaa atgatgctgg caggcgagct caagattatc gtaatcatca
    55441 caattgattg gttgatacgt atcattcatt gacataatcg ctcaccagta agtttgccgc
    55501 agcgtatgct gctttttccc tgacagcctc agaaagggcg tcgtcggcag ccatttcatt
    55561 cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat cctaagtctc cgctggcgat
    55621 ttccgcgtac cgcttgcgta ttaactcaca atatttttcc acatgccctc ctgtcagcac
    55681 tctgacttaa ccgtggatgc aagtctaagc ctacgaagat aaactctgtt tcgcaaggtg
    55741 actataccac actcatttct gcaatatcag cgccgcaact gcacgtattc cgttacaatg
    55801 gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag cgacaattta taaagcgacg
    55861 gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg cctctctgac gctggcgcgc
    55921 catccttcag aaacccagga gcgcatgatg ctgcgcctgc tggcgtggct aaaatatgcc
    55981 gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg atgagccgga agcgtggctg
    56041 cgtaacgatc acctgggcat tgatttgtgg attgagctgg ggctgccgga tgagcgacgg
    56101 attaagaaag cctgcaccca ggccgcagaa gtggcgctgt ttgcctataa tagtcgggcg
    56161 gcgcaaatct ggtggcagca aaatcagagc aaatgtgcgc agtttgccaa tctttccgtc
    56221 tggtatctgg acgacgagca gctggcgaaa gtgagcgcct ttgccgatcg tactatgacg
    56281 ctgcaggcaa cgattcagga tggtgttatc tggttatcgg atgataagaa taatctggaa
    56341 gtgaacttaa ccgtctggca acaaccttca tgattgtgat ttcccgacat gttgctatcc
    56401 ctgatggcga gtttgagatc accgccattc gtgcgcaggg cgcgggcggg cagcatgtta
    56461 ataagacctc aacggctatt catctgcgtt ttgacattcg ggcgtccagc ctgccagagt
    56521 attacaaaga gcgtctgctc gccgccagcc atcatttgat cagcagtgac ggagtgattg
    56581 tcattaaggc acaggaatac cgcagtcagg aactgaaccg cgaagcggcg ctggcccggc
    56641 tggtggcagt gattaaagat ttaacaacag aacaaaaagc ccgacgacct acgcggccca
    56701 cccgtgcatc aaaagagcgc aggctggcat cgaaagcaca aaaatcaagc gtgaaggcga
    56761 tgcgcggcaa agtgcgcagc ggtcgggaat aaaaagaagg aatggatggt gaaaaaagcg
    56821 atagtgacag cgatggctgt aatcagcctc tttactctga tgggatgtaa taatcgggcc
    56881 gaagtcgata cgctttctcc ggcgcaggct gccgaactga aaccgatgcc gcaaagttgg
    56941 cgtggcgtgc tgccgtgtgc tgattgcgaa ggaatcgaaa cctctctgtt cctcgaaaaa
    57001 gacggaacct gggtgatgaa tgagcgttat ctgggggctc gtgaagaacc ttcctccttc
    57061 gcttcctacg gtacatgggc gcgaaccgct gacaagctgg tattaaccga tagcaaaggt
    57121 gaaaagtcat attatcgcgc gaaaggagat gcgctggaga tgctcgatcg tgaaggtaat
    57181 ccgattgaat cgcagttcaa ctacacgctg gaaccggcac aatccagctt acccatgacg
    57241 ccgatgaccc tgcggggcat gtatttttat atggctgatg cggcgacctt cactgattgc
    57301 gcgaccggaa aacgtttcat ggtggcgaat aacgcagagc tggagcgtgg ctacctggct
    57361 gcgcgcggta acagtgaaaa accggtgtta ctgtcagtag aaggtcactt tacgcttgag
    57421 gctaatccgg ataccggtgc gccgaccaaa gtattagcgc ccgatacggc aggtaaattt
    57481 tacccaaacc aggattgcag tagtttgggg ctgtaacccg tcttgagaca gaaacaaatg
    57541 aaatattgcc tcatgcgctg cgcttatcag tcctaaagca tacaatgcaa tattttgaat
    57601 tagcacaatt ttgtaggccg gataaggcgt tcacgccgca tccggcattt tgcgcacgag
    57661 gctggctttt atcactgagt cagcccaaca aagcggcctg ccttgatata aatcccaggc
    57721 ttcatgtgcc caacatactg taactgcgtt tctggcttaa acgcagagat atcgccagta
    57781 cgcatatgca tcagatcggc agggctaatc cagcctgatt gcgccagcgt aagcggatgc
    57841 ccggctttgg caaatgcatc agtgacaaat tccgaacaaa accacgactt tttgtctcct
    57901 tcgcccacac tgcttaactg cgctttcgcc aggccgctga cgcactgttg acgaaaatcc
    57961 tcggagaacg gatttagtga gcacatctgg cgagtcacca taaatggaat aaattcgaca
    58021 atgccgcgat agttgtaacc gctatcttta attttgttgg cgaacgcggt gatttctgtg
    58081 gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa gcttatcact atgctttatg
    58141 gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg cttcggcaac gttattatct
    58201 cccaaataga ttgcaacgtg actcacagag gaagtgctga atacgcggat accaaatgag
    58261 gttaccccaa ggcttgagga gaaaagcaaa tcgccgggtt tgagatctgg ttccgttatt
    58321 tcttttattg attgttcggt gaaagagctt ggatgctgga atttaacagc ccatgttttc
    58381 gcctcggcat ttaccactgt ggctgatgag tctggctggc tgatatcaac ggtacaggcc
    58441 gaaagtaaaa gaaagcaggg gagaagcagg cggcagtacg cctttggttt atccatttta
    58501 tacaatccat gtaaaaaagg gccccgaaat tcaggaccct ttctggcatc agcctttaat
    58561 ctgtttcacc agataatcga cgatgtcacc agtcttaatt aactgtttct cgccgttacg
    58621 acgatattta tattcgatat catcgttgtc gaggttacgg tcgcccagca caatagtgtg
    58681 cggaataccg atcagttcca tatcagcaaa catcacgccc ggacgctctt tgcggtcatc
    58741 cagcagcact tcgatacctt gtgcgcgcag ttcgctgtac agtttatcag caagttcctg
    58801 tacgcggaag gatttgtgca tgttcattgg cagaatcgcc acctggaacg gcgcgatagc
    58861 gtcaggccat acgatgccgc gttcgtcgta gttctgctca atcgccgctg ctaccacacg
    58921 cgttacgccg ataccgtagc aacccatcgt caggatttgg ttacggccat cttcaccctg
    58981 tacggaggct ttcagtgctt cggagtactt ggtacccagc tggaagatgt gaccgacttc
    59041 gataccacgt ttgatcagca gcgtaccctg accatccggg cttggatcgc cagccaccac
    59101 gttgcggata tcagcaattt ccggggtagc gacatcgcga tcccagttga taccgaagta
    59161 gtgtttacca tcgatgttag caccagcagc gaaatcactc atcgccgcaa cggtacggtc
    59221 aatcaccacc ggaatcggca tgtttaccgg acccagtgaa cccggaccgg ctttaaccac
    59281 ggcacgaatt tcttcttcgg tcgcaaaagt cagcgggctg gcaacctgcg gcagtttttc
    59341 tgctttaact tcgttcagct cgtggtcacc gcgcaccagc agcgcaacca gcgggaagct
    59401 actgccttca acggctttaa ccagcagagt cttaaccgtt ttctcaatcg gcagattgaa
    59461 ctgttcaacc agttccgcga tggttttcgc gttcggcgta tcaaccagcg tcatttcctg
    59521 ggtagcagca gcgcgcggtt ctttcggcgc gatagcttct gccagttcaa tgttcgctgc
    59581 atagtcagaa gtatcggaga agaccacatc gtcttcaccg ctctgcgcca gcacctgaaa
    59641 ttcgtgagag gcgctaccgc cgatagaacc ggtatcggcc tgtacggcgc ggaaatccag
    59701 ccccatgcgg ctgaagattt tgctgtaggc cgcatacatt gcatcgtagg tttcctgcag
    59761 ggattcctga gaagtatgga aagagtaagc atctttcatc aggaattcgc gggaacgcat
    59821 gacgccaaaa cgcggacgca cttcgtcgcg gaacttggtc tggatctgat agaagttcag
    59881 cggcagctgt ttgtaagagc tcagctcgtt acgaatcagg tctgtaatca cttcttcatg
    59941 agttgggccg agtacgaacg gacggtcacc acggtcagca atacgcagca attccgggcc
    60001 atactgttcc caacgaccac tctcttgcca cagttcagat ggctgaacca ccggcattaa
    60061 cacctcgatc gcaccggcgt tgttcatctc ttcacgcacg atgttttcga cttttttcag
    60121 aacgcgcacg ccggtcggca gccaggtata taacccggag gccagcttgc ggatcatccc
    60181 ggcgcgcagc atcagctgat ggctgatcac ctcggcgtcg gcaggtgtct ccttgagagt
    60241 ggagagcagg tattggctag tacgcatgtt gttacggttc cagttggaag gtagaacagg
    60301 ctcaaggcga gcctgggaca aaaaaagtga tttagtttac cagtgcaaaa gaaatgtcaa
    60361 aagagaaggg cgtgaattta acgcggttcc agcgcaaaga cttcaaaacc tgcgtcggtg
    60421 acgcgccagc gaacgttaaa atcatgcagc cagacggcat aggttttgcc cgtttcctca
    60481 cctttacgat aggccgggcg cgggtcctgc gccagtactt cgcggataaa cagcgttaac
    60541 tgcggataac gcttcttcag cgtcaaaagc tgcttttcga cctctgcggt aaaacacacc
    60601 gccatttctg ccgctggcgc actttgcgca tagctggcac tggcatcggg aagcgattca
    60661 gcaaagggga gatacggttt gatatccact accggcgtac catcgaccag atccagactg
    60721 ccgagcttca gaatcacgct atctttatgg caaacaacct ctttcagctc taccagcgac
    60781 ataccaatcg ggttagggcg aaaagtggag cgcgtagcga aaacacccat tctggcatta
    60841 ccgccgagac gaggcggacg cacagtcgga cgccagccgc cttccatcgt ttgatgaaag
    60901 acgaaaagga tccataaatg gctgaatgct tccaggccgc gaacggcgtc ggcctggttg
    60961 tagggagcaa tgagatgtag ttcgccgttg gcgcttttta ccagacctgg ctggcgcgga
    61021 acggcgaatt tttctttata gggcgagcga ataacgccta tttgctcgaa ctggaaactg
    61081 ctcatttcgc cgtgatgtta agcgcagaac cgatacatac agcctgacga tagcaacctg
    61141 gcgtaccgct ggtgacttcg cagctatgca gtaataccgc attggctttc attttcgagg
    61201 cgttgatttg catccgctta cgtgcggttg gaatgctcgg cggagagtcc tgattagagg
    61261 cctggcaaga gtcgccactg acttcaccga gatcgcggaa cggtttgccg actaattctt
    61321 ctgcattggt ataaattcgg accggcgtgg cgcgcggcgc tttcggtttt gcaggctccg
    61381 ctttcggctg gggtgcagtg ctttgaacgg gttcgacagg ggatctgctt aacatggaac
    61441 agccgcttag catgagtgct actaaacaga tcggtaaagc acgcatagta tttcctcaat
    61501 gtatgatcaa aacgtcaata ttgaatcagg cgcttgtaaa aatgacaaga cgggcaagcg
    61561 cccgtcctga atgatattac aaattgtgga aacagcctaa atattaccag cctttaacag
    61621 cgccgccgtt aaacactttg tttgctgctt cgtaaacttc gtcagactga taagcctgaa
    61681 cgaatttctt cacgttttcg gcgtctttgt tgtcttcacg cgttacgatc aggtttacgt
    61741 acggggactc tttatcttcg acaaagatac cgtctttcgc tggagtcagg ccaatctggc
    61801 tggcataggt ggtattgata actgccagag cgatttgcgc gtcgtccaga gagcgcggca
    61861 gctgcggtgc ttccagttca acaattttca gattttttgg gttctcaaca acatcaagaa
    61921 cggtcggcag caggccaacg ccatctttca gtttgatcaa gcccactttt tgcagcagca
    61981 gcagtgaacg accaaggtta gttgggtcgt ttggcacggc aacctgcgaa ccatcctgca
    62041 gttcatccag tgatttgatt ttcttggagt aaccagcaat cggataaaca aatgtgttgc
    62101 ctactgcgac cagtttgtag ccacgatctt tcagttgctg atcaaggtac ggtttatgct
    62161 ggaaggcgtt ggcgtcgata tcgcctttgc tcaatgcttc gtttggcaga acatagtcgt
    62221 tgaaggttac cagctcaacg tccaggccat atttgtcttt cgcaactttc tgcgcgactt
    62281 ctgcaacctg ctgttcggca ccaacaatca cgccgacttt aatgtggttt ggatcttttt
    62341 catcctgacc gcagcctacc agtgccagtg atccaatcag ggctcccact gccgcaaagg
    62401 ttttgaattt gaacgccata ccttattcct tcttcaatta tttatgttgt gttgaacgtt
    62461 acttgcgagt gacagcccgg acgatgcggt cgcctgcgaa ctgaattaaa taaaccagaa
    62521 tgaccagcaa taccagtacc gtattcatca ccgtcgcgtt gtagccgatg tagccatact
    62581 gatagccaat ctgacctaaa ccaccggcac cgactgcacc acccatcgcg gaataaccaa
    62641 ccagggtaat cagggtaata gttgccgcat ttaccagacc cggcaacgct tccggtaaca
    62701 gcactttacg gacgatctgc attggcgtgg cacccattgc gcgggaagct tcaattaacc
    62761 cggttgggat ctccagcaga gcattctcga ccatacgggc aataaacggt gctgcaccaa
    62821 cggttaacgg aacaatcgct gcctgcaaac caatcgatgt accaacaata acgcgggtaa
    62881 acggaatcat ccataccagc aagataatga acgggatgga acggaaaatg ttcacaatcg
    62941 cagaaacggt acgatacagc ttcgcgttag caataatttg ccccggacgc gtgacataaa
    63001 gcaggacgcc aaccggcaga ccaatcacaa atccaaaaaa accggatacg aaggtcattg
    63061 ccagcgtttc ccatacgcca cgaaccagca gccacatcat cggctcagac ataacccagt
    63121 acctctactt ttacatggtg ttcctgcagc caggtaatgg cggcttgcgt atcttgttgt
    63181 gtgccgtgca tttcagtcag catgatgccg aacttaacgc caccggcgta atccatctgc
    63241 gcgctaataa tgttgttgtt gacgttgaaa cgacgcgcgg tttcagaaag cagtggggca
    63301 tcgaccgatt gaccggtaaa ctccagacgc aacatcggca cgcagtcagt gaatggctcc
    63361 gcttgcagac gttcctggta atcttccggg atatccagat gcagggtcga ctgaataaac
    63421 ttctgcgcca gcggcgtttt cggatgcgag aacacttcac ttaccgtgtc ctgctcgatc
    63481 agttcgccat tgctgatgac cgccacgcaa tcacaaatac gcttcacaac gtccatttcg
    63541 tgggtgatca acagaatcgt caaacccaga cggcggttga tgtctttcag cagttcgaga
    63601 atagaacgtg tcgttgccgg atccagcgcg ctggtggcct catcacacag caatactttg
    63661 ggattgctgg ctaacgcacg ggcaatcgcc acacgctgtt tctgcccacc ggaaagattc
    63721 gacgggtagc tatcatgctt atcgccaaga ccaaccaatg acagcaattc cgtcacgcga
    63781 cgtttgatct catctttcgg tgtgttgtcc agctccagcg gcagagccac gttgccaaaa
    63841 acagtacgcg aagagagcag gttaaaatgc tggaaaatca taccaatctg gcggcgagtt
    63901 ttggtcaact cggattctga cagcgtggtc agttcctggc catcgaccag cacgctaccc
    63961 tcggttgggc gctccagcag gtttacacaa cgtataagcg tactcttacc cgcgccagag
    64021 gcaccgataa cgccataaat ttgtccagct ggcacatgca ggctgacgtt gttcaacgcc
    64081 tggatggtgc gggtgccctg gtggaacact ttggtgatat tcgaaagttt tatcattgat
    64141 tatttattat cgtcattaag ttagtcgtgg catttcgaat gcctggaacg ggcaacgcct
    64201 tcaatgaaat ggatgttaag gcatccagac gtctaaatca atcaggttta tgcgaagagc
    64261 actttcttgc tggtcgaaac atgcgatact agcgtcacat gccttattaa ggagctataa
    64321 aaggtggcga agagcgtacc cgcaattttt cttgaccgtg atggcaccat taatgtcgat
    64381 cacggctatg tccatgagat cgacaacttt gaatttatcg acggtgttat tgacgccatg
    64441 cgcgagctaa aaaaaatggg ctttgcgctg gtggtagtaa ccaaccagtc tggcattgct
    64501 cgcggtaaat ttactgaagc acagtttgaa acgctgaccg agtggatgga ctggtcgctg
    64561 gcggaccgcg atgtcgatct ggatggtatc tattattgcc cgcatcatcc gcagggtagt
    64621 gttgaagagt ttcgccaggt ctgcgactgc cgcaaaccac atccggggat gcttttgtca
    64681 gcacgcgatt atttgcatat tgatatggcc gcttcttata tggtgggcga taaattagaa
    64741 gatatgcagg cagcagctgc ggcgaacgtg ggaacaaaag tgctggtgcg tacgggtaaa
    64801 cctgttacgc ctgaagcaga aaacgcggcg gattgggtgt taaatagcct ggcagacctg
    64861 ccgcaagcga taaaaaagca gcaaaaaccg gcgtaatgat taaaagatga gcggttgaaa
    64921 taaaaatgca tttttccgct tgtcttcctg agccgac
//
LOCUS       DADSZD010000004        63471 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.004, whole genome shotgun sequence.
ACCESSION   DADSZD010000004 DADSZD010000000
VERSION     DADSZD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.004"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(81..233)
                     /locus_tag="J1Z28_000190"
     CDS             complement(81..233)
                     /locus_tag="J1Z28_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA7715064.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(498..1232)
                     /gene="cysH"
                     /locus_tag="J1Z28_000191"
     CDS             complement(498..1232)
                     /gene="cysH"
                     /locus_tag="J1Z28_000191"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBA7715065.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1306..3018)
                     /gene="cysI"
                     /locus_tag="J1Z28_000192"
     CDS             complement(1306..3018)
                     /gene="cysI"
                     /locus_tag="J1Z28_000192"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBA7715066.1"
                     /translation="MSEKHPGALVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREVGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3018..4817)
                     /gene="cysJ"
                     /locus_tag="J1Z28_000193"
     CDS             complement(3018..4817)
                     /gene="cysJ"
                     /locus_tag="J1Z28_000193"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBA7715067.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLAASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5133..5498
                     /gene="queD"
                     /locus_tag="J1Z28_000194"
     CDS             5133..5498
                     /gene="queD"
                     /locus_tag="J1Z28_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBA7715068.1"
                     /translation="MMSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGE
                     VDPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPL
                     LSAVMVKETCTAGCIYRGE"
     gene            5576..6847
                     /locus_tag="J1Z28_000195"
     CDS             5576..6847
                     /locus_tag="J1Z28_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7715069.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLLPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLIWRHLRRHGLWHLTGDVIRSIRCL"
     gene            6838..7098
                     /locus_tag="J1Z28_000196"
     CDS             6838..7098
                     /locus_tag="J1Z28_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBA7715070.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GGLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7115..7690
                     /locus_tag="J1Z28_000197"
     CDS             7115..7690
                     /locus_tag="J1Z28_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBA7715071.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7837..8697)
                     /locus_tag="J1Z28_000198"
     CDS             complement(7837..8697)
                     /locus_tag="J1Z28_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBA7715072.1"
                     /translation="MNIAIVTINQDHAAIASWLAAQDFSGCTLAHWQIEPQPMIAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELATRLAWRVHGASICQVISFDISTASVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPSGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKAVLEA
                     LVTTIHADCQ"
     gene            complement(8694..9473)
                     /locus_tag="J1Z28_000199"
     CDS             complement(8694..9473)
                     /locus_tag="J1Z28_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBA7715073.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLTQRKNGTSMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLELIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDAPFI
                     TLEQRTENGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9451..10860)
                     /locus_tag="J1Z28_000200"
     CDS             complement(9451..10860)
                     /locus_tag="J1Z28_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715074.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFIAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVATATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLAAVLLVGFVVTWLWAPETKA
                     LPLVAAGNAGGANEHSVSV"
     gene            complement(10882..12336)
                     /locus_tag="J1Z28_000201"
     CDS             complement(10882..12336)
                     /locus_tag="J1Z28_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA7715075.1"
                     /translation="MSLSRVAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPYADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12406..13191)
                     /locus_tag="J1Z28_000202"
     CDS             complement(12406..13191)
                     /locus_tag="J1Z28_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7715076.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13510..14787
                     /locus_tag="J1Z28_000203"
     CDS             13510..14787
                     /locus_tag="J1Z28_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715077.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGMRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRNTNNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            14814..16292
                     /locus_tag="J1Z28_000204"
     CDS             14814..16292
                     /locus_tag="J1Z28_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7715078.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKTWAWSED
                     AAVMEKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPTRRNAINGCASLGAAINTAVGLGPYPDYTKAVDKMVRVKDIFIPIESNAKRY
                     DAMNKSIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
     gene            16457..17377
                     /locus_tag="J1Z28_000205"
     CDS             16457..17377
                     /locus_tag="J1Z28_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HBA7715079.1"
                     /translation="MSTRVWVLGDAVVDLLPESQGRLLQCPGGAPANVAVGIARLGGE
                     SAFIGKVGDDPFGRFMYQTLSKENVDTRYMSLDPQHRTSIVAVGLDEQGERNFTFMVR
                     PSADLFLQPDDLPTFGPGEWLHLCSIALSAEPSRSTAFLAMEKIRQAGGNISFDPNIR
                     SDLWQSEALLRKYLDRALSLANIAKLSEEELLFISGESQVQQGAYSLVQRYSLTLLLI
                     TQGKNGVLVYFQGQFIHYPAKPVSVVDTTGAGDAFVAGLLAGLADSGIPTNTRQLERI
                     IAQAQICGALATTAKGAMTALPRQHDLPSQ"
     gene            17545..19062
                     /locus_tag="J1Z28_000206"
     CDS             17545..19062
                     /locus_tag="J1Z28_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBA7715080.1"
                     /translation="MYKKTTLAVLIALLTGATTVHAQTDISSIESRLAALEQRLKNAE
                     SRAQAAEARAKTAELQVQKLAETQQQNQLTTQEVAQRTVQLEQKSAENSGFEFHGYAR
                     SGLLMNDAASSSKSGPYLTPAGETGGAVGRLGNEADTYVELNVEHKQTLDNGATTRFK
                     AMLADGQRDYNDWTGGSSNLNIRQAFAELGALPSFTGAFKDSTVWAGKRFDRDNFDIH
                     WLDSDVVFLAGTGGGIYDVKWNDTFRSNFSLYGRNFGDLDDIDNNVQNYILTMNHYAG
                     PFQLMVSGLRAKDNDDRKDANGDLIQTDAANTGVHALVGLHNDTFYGLREGTAKTALL
                     YGHGLGAEVKGIGSDGALLSEANTWRFASYGTTPLGSGWYVAPAILAQSSKDRYVKGD
                     SYEWVTFNTRLIKEVTQNFALAFEGSYQYMDLKPKGYQNHNAVNGSFYKLTFAPTLKA
                     NDINNFFSRPELRLFATWMDWSSKLDDFASNDAFGSSGFNTGGEWNFGVQMETWF"
     gene            19123..20493
                     /locus_tag="J1Z28_000207"
     CDS             19123..20493
                     /locus_tag="J1Z28_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HBA7715081.1"
                     /translation="MDFDKIAQSLLPLLGGKENIASAAHCATRLRLVLVDDTLADQHA
                     IGQIDGVKGCFRNSGQMQIIFGTGVVNKVYAAFIQVAGISESSKADTARLAAQKLNPF
                     QRIARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVNADNAIYILLDMCSSAAFIIL
                     PILIGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFQTMNFFGFEIAMIGYQG
                     TVFPVLLAVWFMSIVEKQLRRFIPDALDLILTPFLTVVISGFIALLIIGPAGRALGDG
                     ISFVLSTLIAHAGWLAGLLFGGLYSAIVITGIHHSFHAIEAGLLGNPAIGVNFLLPIW
                     AMANVAQGGACLAVWFKTKDTKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAAL
                     IGGAAGGAWVVSVHVYMTAVGLTAIPGMAIVQPTSLVNYIIGMVIAFAVAFSLSLLLK
                     YKTDEE"
     gene            20493..21902
                     /locus_tag="J1Z28_000208"
     CDS             20493..21902
                     /locus_tag="J1Z28_000208"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBA7715082.1"
                     /translation="MSMPSQMPAILQAVMKGQSKALADAHYPCWHLAPVTGLMNDPNG
                     FCWSGGRYHLFYQWNPLACDHKYKCWGHWSSTDLLHWQHEPLALMPDKEYDRNGCYSG
                     SAVNNQGVLTLCYTGNVKFDDGSRTAWQCLATENNQGGFDKLGPVIPLPDGYTGHVRD
                     PKVWKHNSQWYMVLGAQDKEKRGKVLLYSSVDLYTWSFHGEIAGNGLNEIDNAGYMWE
                     CPDFFALDGEYILLCCPQGMVREHERYLNTYPCAWLHGQFDYETGKFTHGAFSELDAG
                     FEFYAPQTMEAPDGRRLLVGWMGVPDGEEMLQPTRKHHWQHQMTCFRELSFQQGKLFQ
                     MPIRELAQLREAEHFWQGKADHAPHVEIERLEMDIIPSGELYLNFGNALALHLNDDGI
                     QLQRRSLAGQEKLTRYWRGSVTSLKILCDSSSVEIFINNGEGVMSNRYFPHHPASLIL
                     QGESDVTLRYWSLRACMVE"
     gene            21928..22935
                     /locus_tag="J1Z28_000209"
     CDS             21928..22935
                     /locus_tag="J1Z28_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7715083.1"
                     /translation="MRKTKRVTIKDIAELAGVSKATASLVLNGRGKELRVAQETRERV
                     LSIAQQQHYQPSIHARSLRDNRSHTIGLVVPEITNYGFAVFSHELETLCREAGVQLLI
                     SCTDENPSQETMVVNNMIARQVDGLIVASSMLHDNDYQKLSEQLPVVLFDRHMNGSTL
                     PLVITDSVSPTAALVADIARSHPDEFYFLGGQPRLSPTRDRLEGFTQGLQQAGVTLQP
                     EWIIHGNYHPSSGYEMFAALCARLGRPPKALFTAACGLLEGVLRYMSQYNLLDSKIHL
                     ASFDDHYLYDSLSVRIDTIQQDNRQLAFHCFELISQLIEGETPSPLQRYLPASLQKRY
                     R"
     gene            complement(23020..23691)
                     /gene="queE"
                     /locus_tag="J1Z28_000210"
     CDS             complement(23020..23691)
                     /gene="queE"
                     /locus_tag="J1Z28_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBA7715084.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQTHK
                     YLNIA"
     gene            23864..24466
                     /locus_tag="J1Z28_000211"
     CDS             23864..24466
                     /locus_tag="J1Z28_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HBA7715085.1"
                     /translation="MLRIFIAIIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRT
                     DLIPNLVASIKGYSSHERDVLEAVTLARSQANRASSDLQQTPGDEQKLQTWQQAQAQV
                     SRTLGQLTIISERYPELKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVAAVSKAPTIDFSQNTNAH"
     gene            24480..25385
                     /locus_tag="J1Z28_000212"
     CDS             24480..25385
                     /locus_tag="J1Z28_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA7715086.1"
                     /translation="MRIFIFLMAFFCSSVFATQIPVPESPRYVNDLTGKLTNDEVNTL
                     TNQIKELTQKSHAQLVVLVVETTGDETIEQYATRVFDSWKPGDKDRDDGVLLLVAWQD
                     HTVRIEIGYGLEGVITDAQSGKIIRNSIIPAFKNGDLAGGLQKGISDIDNRLTGNNLA
                     TITPTDHPLPFNGWWALLVWAIVLTFISARGYFKTLGLICFTAIVLAFVLPIAGFSGS
                     WGVLATLLCFATPFLAVAIAFTPFGKKVRDSMRSANRPSRYERSNSASWRSSSSSSSS
                     SSSSFDNDNFSGGGSSGGGGASGRW"
     gene            25400..26548
                     /locus_tag="J1Z28_000213"
     CDS             25400..26548
                     /locus_tag="J1Z28_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA7715087.1"
                     /translation="MRIFLLLLMLYGFQALAEQLPSVTDLTGTLTTEEQTALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQSYGLYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLKEMLPPEQAAQIVHYHMQPEFKKNNLFSGLTQGIEGIAQFTYIKANLSPL
                     DALANHLFANPQRSLPCLAWTLLMIVAIVVLWRFTSRPGPGIWMISMITPMVWFFFFQ
                     DDVIIRRVSLVCFSLFFAATCWQRIAVIFHMYRVFPMMLAPKNKNAKVKKQKTTQQVS
                     QSERNSLFVVLLIIVVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGHKSNRKTGNRNAHSRSSSKNSFRGGGGSSGGGGSSDRW"
     gene            26554..27426
                     /locus_tag="J1Z28_000214"
     CDS             26554..27426
                     /locus_tag="J1Z28_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBA7715088.1"
                     /translation="MRIILFLLTLWCLPVLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITQKTRAQVAVLIVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(27486..28784)
                     /gene="eno"
                     /locus_tag="J1Z28_000215"
     CDS             complement(27486..28784)
                     /gene="eno"
                     /locus_tag="J1Z28_000215"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBA7715089.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(28872..30509)
                     /gene="pyrG"
                     /locus_tag="J1Z28_000216"
     CDS             complement(28872..30509)
                     /gene="pyrG"
                     /locus_tag="J1Z28_000216"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBA7715090.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(30737..31528)
                     /gene="mazG"
                     /locus_tag="J1Z28_000217"
     CDS             complement(30737..31528)
                     /gene="mazG"
                     /locus_tag="J1Z28_000217"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBA7715091.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(31600..31935)
                     /gene="mazF"
                     /locus_tag="J1Z28_000218"
     CDS             complement(31600..31935)
                     /gene="mazF"
                     /locus_tag="J1Z28_000218"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBA7715092.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(31935..32183)
                     /gene="mazE"
                     /locus_tag="J1Z28_000219"
     CDS             complement(31935..32183)
                     /gene="mazE"
                     /locus_tag="J1Z28_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBA7715093.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(32261..34495)
                     /locus_tag="J1Z28_000220"
     CDS             complement(32261..34495)
                     /locus_tag="J1Z28_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBA7715094.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(34543..35844)
                     /gene="rlmD"
                     /locus_tag="J1Z28_000221"
     CDS             complement(34543..35844)
                     /gene="rlmD"
                     /locus_tag="J1Z28_000221"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBA7715095.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVEPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            35901..38657
                     /gene="barA"
                     /locus_tag="J1Z28_000222"
     CDS             35901..38657
                     /gene="barA"
                     /locus_tag="J1Z28_000222"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBA7715096.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSLPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(38888..40228)
                     /gene="gudD"
                     /locus_tag="J1Z28_000223"
     CDS             complement(38888..40228)
                     /gene="gudD"
                     /locus_tag="J1Z28_000223"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBA7715097.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(40249..41589)
                     /locus_tag="J1Z28_000224"
     CDS             complement(40249..41589)
                     /locus_tag="J1Z28_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase-related protein"
                     /protein_id="HBA7715098.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVEAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGQALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(41591..42943)
                     /locus_tag="J1Z28_000225"
     CDS             complement(41591..42943)
                     /locus_tag="J1Z28_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715099.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVVGQ
                     "
     gene            complement(43377..43826)
                     /locus_tag="J1Z28_000226"
     CDS             complement(43377..43826)
                     /locus_tag="J1Z28_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBA7715100.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(43844..44626)
                     /gene="truC"
                     /locus_tag="J1Z28_000227"
     CDS             complement(43844..44626)
                     /gene="truC"
                     /locus_tag="J1Z28_000227"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBA7715101.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDLIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(44626..44955)
                     /locus_tag="J1Z28_000228"
     CDS             complement(44626..44955)
                     /locus_tag="J1Z28_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBA7715102.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(45577..46122)
                     /gene="syd"
                     /locus_tag="J1Z28_000229"
     CDS             complement(45577..46122)
                     /gene="syd"
                     /locus_tag="J1Z28_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBA7715103.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            46190..47038
                     /gene="queF"
                     /locus_tag="J1Z28_000230"
     CDS             46190..47038
                     /gene="queF"
                     /locus_tag="J1Z28_000230"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBA7715104.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVCQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            47150..48514
                     /gene="ppnN"
                     /locus_tag="J1Z28_000231"
     CDS             47150..48514
                     /gene="ppnN"
                     /locus_tag="J1Z28_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBA7715105.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            49071..50360
                     /locus_tag="J1Z28_000232"
     CDS             49071..50360
                     /locus_tag="J1Z28_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7715106.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            50418..51785
                     /gene="sdaB"
                     /locus_tag="J1Z28_000233"
     CDS             50418..51785
                     /gene="sdaB"
                     /locus_tag="J1Z28_000233"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBA7715107.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            51807..52652
                     /gene="xni"
                     /locus_tag="J1Z28_000234"
     CDS             51807..52652
                     /gene="xni"
                     /locus_tag="J1Z28_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311685.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBA7715108.1"
                     /translation="MRGLFPISHPAIACSGIECYPYRLIFKGVIVAVHLLIVDALNLI
                     RRIHAVQGSPCVETCQHALDQLIMHSQPTHAVAVFDDENRSSGWRHQRLPDYKAGRPP
                     MPEELHNEMPALRAAFEQRGVPCWSASGNEADDLAATLAVKVTQAGHQATIVSTDKGY
                     SQLLSPTLRIRDYFQKRWLDAPFIDKEFGVQPQQLPDYWGLAGISSSKVPGVAGIGPK
                     SATQLLVEFQSLEGIYENLDAVAEKWRKKLETHKEMAFLCRDIARLQTDLHIDGNLQQ
                     LRLVR"
     gene            complement(52757..53905)
                     /gene="fucO"
                     /locus_tag="J1Z28_000235"
     CDS             complement(52757..53905)
                     /gene="fucO"
                     /locus_tag="J1Z28_000235"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBA7715109.1"
                     /translation="MANRMILNETAWFGRGAVGSLTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(53933..54580)
                     /gene="fucA"
                     /locus_tag="J1Z28_000236"
     CDS             complement(53933..54580)
                     /gene="fucA"
                     /locus_tag="J1Z28_000236"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBA7715110.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            55127..56443
                     /gene="fucP"
                     /locus_tag="J1Z28_000237"
     CDS             55127..56443
                     /gene="fucP"
                     /locus_tag="J1Z28_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBA7715111.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            56476..58251
                     /gene="fucI"
                     /locus_tag="J1Z28_000238"
     CDS             56476..58251
                     /gene="fucI"
                     /locus_tag="J1Z28_000238"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBA7715112.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSSESIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            58360..59778
                     /gene="fucK"
                     /locus_tag="J1Z28_000239"
     CDS             58360..59778
                     /gene="fucK"
                     /locus_tag="J1Z28_000239"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBA7715113.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYVGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            59780..60202
                     /gene="fucU"
                     /locus_tag="J1Z28_000240"
     CDS             59780..60202
                     /gene="fucU"
                     /locus_tag="J1Z28_000240"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBA7715114.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            60260..60991
                     /gene="fucR"
                     /locus_tag="J1Z28_000241"
     CDS             60260..60991
                     /gene="fucR"
                     /locus_tag="J1Z28_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBA7715115.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIKLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(61035..62135)
                     /gene="rlmM"
                     /locus_tag="J1Z28_000242"
     CDS             complement(61035..62135)
                     /gene="rlmM"
                     /locus_tag="J1Z28_000242"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBA7715116.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(62128..62523)
                     /locus_tag="J1Z28_000243"
     CDS             complement(62128..62523)
                     /locus_tag="J1Z28_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBA7715117.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(62542..63459)
                     /locus_tag="J1Z28_000244"
     CDS             complement(62542..63459)
                     /locus_tag="J1Z28_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HBA7715118.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
BASE COUNT        15588 a        16454 c        15438 g        15991 t
ORIGIN      
        1 taaacaatat aaattaaaaa agggtgcagg agaccaaacc atcacaattc agtacgcggg
       61 gttttacccc cgcgtggtgg ctacttcttc gattcgtaag cgagaactgc cttaaactcc
      121 ataccacgtt ctctgatact caactcacac aacgagtcgc ctaccatcag cgtgaatccc
      181 agtactgtac aacacagtac gatgattgcc acaagggcat attttgtcag catgtcttgc
      241 ctccctgcaa agaagagact aacattcgaa ttgtttgggt tcgaacgtaa gcctcaggtt
      301 gatagaaata tcgcctgggg cttttgtccg tctggaacct cgcgaatgct caacgccaga
      361 cggcctcaag cacccaacgc cattctatgc ctgttgattc ttcctgcggc gttcacgttt
      421 aaaatcagga atcctgctcg caaaataaaa acgcccccat cttttcagac agaggcgttt
      481 aatttaagca gcaatattta cccttcgtgt aatccacatt cccttttcaa gccaaagaaa
      541 cgcgtttctt cttccgccat gccgggttcc catttacggg ttgtatgggt atctcctacc
      601 gataaatatc cttcatccca taatgggtga tatttcaggc catgtttttg caggtactga
      661 tatatcgtcc gattatccca gtcaataatc ggcagcacct taaatacgcc acgctgaatt
      721 gccagcaccg gtaaattggc gcggctgccg gattgttcac ggcgcaggcc agcaaaccag
      781 gtttgcgcat tcagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg
      841 tacttttcaa tgccttcaac gccctgctcc cacagtttgc cgtagcgtgc ttcctgccag
      901 gcggcgcttt cggtagcacg atacactttc aggttgagct tgagtttgtc cgttaactcg
      961 tcaataaagc ggtaggtttc cgggaacaga tagccggtat cggtgaggat cactggaata
     1021 tccgggcgaa tctgattcac cagatgcaga ctcaccgccg cctgaatgcc aaagctggaa
     1081 gaaagtacat attcaccggg cagattatcc agcgcccagg ctacgcggcc ttcagcgtcc
     1141 agtttttcca gttgggcgtt agtttccgcc agcgccagaa tccggtctac ctttggcagt
     1201 tcgttcaggg cgtttagatc gagtttggac ataagttcct cactgtttgc cttgcctgat
     1261 gcaacgctta cacatcttat caggccaaca agaccgggct gatggttaat cccacaaatc
     1321 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     1381 ttcacccact tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     1441 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     1501 gccaagatgc aggttgtagc gacccggcgc tttccccacc agacccactt ccgccagcat
     1561 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     1621 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc
     1681 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccattgagt tttcacgctg
     1741 cggcgtgacg gcattcatta acccgctttc tttggcgatc ttctcgatct tcgctttctc
     1801 gctttccggc acgccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt
     1861 gtggatcttc gcgatctcca gcaagccggt tttcagcgga cgtcccggat aatcaagaat
     1921 gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc
     1981 aatacgatct ccgcgtccgg taaactcata cgggcgaatc ggctcgaatt tgatccccgc
     2041 gcgacgttca acttccgctt taaacgtctc aacccccacg cgctccagcg tgtatttggt
     2101 tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc
     2161 cgccaccgcc agcgtatgct ccagcggcag atagccaaac tcactcgccg tgcgggcgta
     2221 ggttttcttg ttgccgtgtt cgatggaaag cccaccgccc accagcaggt taaagcccac
     2281 cagcttgccg ttttcggcga tcgccacgaa gttcatgtcg ttggcgtgca gatcgatatc
     2341 gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag
     2401 gatcggctct tcatcagtag tggcgacttt ttcctgatcg agccagatct ccgcatacgc
     2461 gcgggtacga ggcagcagat gttcagaaat tttcttcgcc cattcatatg cttccgcatg
     2521 cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttggcggt
     2581 cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt
     2641 cagaatgccg tggaactgga atgtctggcg gttggtcagg cgaatgctgc catagatggt
     2701 gttttcaccg gcaaatttgt cgatcgcctg ccactgttta gtggtaatca ccccacccgg
     2761 cagacgacag cgaagcagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     2821 gcgaatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc
     2881 gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     2941 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaagcccc
     3001 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca
     3061 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     3121 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     3181 ccgtcattga tccagcgcca cagctctgcg ccctgttcgc gcagtttgtc ttgtacgtaa
     3241 attttttctt tttgatcgcg cgaccaggcg agatcgatgc gcgtcagcac gccttctttg
     3301 acgtaacgct gccattccac ctggtagagg aaatcttccg taaagtgtgg gttgccaaag
     3361 aatagccagt ttttacccgg cgcttcgtcg gcagcacgct gctgcataaa ggcacggaac
     3421 ggcgcgatgc cggtgccagg gccaatcatg atcaccgggg tttccggatt ggttggcagg
     3481 cggaagttgt cgttatgttc gataaatacg cggacttcac cctcttcttc cacacgatcc
     3541 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     3601 ccaacggtaa cgtgcacttc gttctcgact tccgcctgcg aggaggcgat ggaatacagg
     3661 cgcggggtca gcgggcgcag cagattaatc agcgcttcgg catccagttg cgccggagag
     3721 aaacgcacca tgtcgacaat cggcgtcgtc gcggcgtaat gctgtaactt cgctttatcg
     3781 cccaccagcg gcagcagcgt ttcgctgcgg gtaagcgtgg catagttctc aacaatgttg
     3841 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     3901 ccctcgacgg tgacaggttc atcgcctttc agccacagca gttcgacaag ttctttcacc
     3961 agtgccggat cgttctgata ccagacgccc aacgcgtcac ccggctggta acgcaggcca
     4021 gagtcaccta agtcaatttc aatgtgacga acgtcttttt cagagttacg cccggtaatt
     4081 ttctggttaa ccgaaaggct ggccgccagc ggcgcgtctt tgctatacgg gctggtgtgg
     4141 atttcattta ccgtgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     4201 ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacgtcg
     4261 gcatcgacac ggtcgagcag gcgttcaccg cccagttccg ccagcttact gtcaaaatct
     4321 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg
     4381 gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     4441 tccggcggtt ccccttcccc ttgcgttgac gtcaccacga taagcagttt ttcgctggcg
     4501 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgccgctaat
     4561 aaatcatcgc gtaatgcttc agcaacccga cgcgcattgc cagtttgcga ggcggagata
     4621 atagttatac ccggcatttc tgcggcgggc gctggcgtcg ctgcaagcgc agcaggctgc
     4681 tggttgagca cgccccagaa atagccagaa acccaggcaa gctgggtagg agttaaatcg
     4741 gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt
     4801 gggacctgtg tcgtcatgcg ttgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     4861 cagcgcccat aaaacagaag agaaggtaag gttaacgggg taaacggtgt ggattaaaga
     4921 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     4981 caaaatagat taaccaaccc aatgaaaaac aaatgaatat tgcccatcaa taagataaat
     5041 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     5101 ctacggcggt tttatcgtca tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     5161 tcaccttcga agccgctcac cgcttgccgc acgtcccaca agggcataaa tgtggtcgcc
     5221 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     5281 gctggattat cgatttcgct gaactgaaag cggcgtttaa accaacttat gagcgcctcg
     5341 atcaccatta tctcaatgat attcctggtc tggaaaaccc aaccagcgaa gttttagcaa
     5401 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
     5461 aaacctgcac cgcaggttgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
     5521 ttcccttaag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
     5581 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
     5641 ctgcgcgcga gcgggtttat ccgttttgtt actggaacga gcggagattc ccggcagcaa
     5701 aaatctttcc ggcgggcgtt tatataccca tgcgctcgcg gaactcctcc cgcaatttca
     5761 cctgaccgcg cctcttgaac gacgcatcac tcacgaaagt ctttctctgt taacgccaga
     5821 tggcgcaacg acgttttcca gcttactgcc cggcggtgaa tcctggagtg tattacgcgc
     5881 acgattcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg
     5941 tgcgacggta gatgcactgt atgaagaaaa cggcagggtg tgtggtgtca tttgtggtga
     6001 cgatattctt cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctcgcgga
     6061 acgccacggg ttagtgactc gtcctgctgg cgaagcgatg gcattgggga tcaaagaagt
     6121 gctgacgctg gaaccatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
     6181 agcgttgttg ttcagcggcg ggatctgtga tgacttaccc ggcggcgcat ttctttatac
     6241 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcctccctta cgcaaagtcg
     6301 tgttccggca agcgagctgc tggctcgctt taaaacacat ccggcagtgc gcccgcttat
     6361 caaaaacacg gaatcactgg agtatggtgc gcatctggtg ccagaaggtg gcctgcgcag
     6421 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
     6481 cgtcaatacc ggaatttccg tgcgcggtat ggatatggcg ctaactggcg cgcaggcggc
     6541 ggcacaaacg ctgataagcg cctgccagca ccgcgagccg caaaatctgt ttccgcttta
     6601 tcatcacaac gtagagcgca gcctgctgtg ggatgttcta cagcgttatc agcatgttcc
     6661 ggcgcttttg caacgccctg gctggtatcg ggtgtggcct gggttaatgc aggatatttc
     6721 ccgtgattta tgggatcagg ggaatacacc tgttccaccg ctgcgccagt tgatctggcg
     6781 tcatttacgt cgtcacggct tgtggcatct gacgggcgat gttatcagga gtattcgatg
     6841 tctgtagccc gtaatctctg gcgcgctgct gatgcgccgc acattgttcc ggctgactcc
     6901 gttgagcgcc agacggcaca acggttgatt aacgcttgtc cggctggtct tttttcgctc
     6961 acacctgaag gtggcttacg tgttgattat cacggctgcc tggagtgtgg cacctgccgt
     7021 ttgctgtgcg acgaatcaac actacaacag tggcgctatc cgccttccgg attcggcatc
     7081 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca
     7141 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcaa tcgactctga
     7201 atgccagttt atttccgtgt tgtacggcaa tatctgcacc attagtaata tcgttaaaaa
     7261 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
     7321 taaagaagtg gtgattcagt ttttgaaact ggtaaccgag gcggacggaa ttatcagtac
     7381 caaagcctca atgctgaaag cagcaagagc ggaaggtttt ttctgtattc atcgactgtt
     7441 tattgttgat tcgatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
     7501 ttgtattgag atcctgccag gctgcatgcc taaagtgctg ggctgggtga cggagaaaat
     7561 ccgccaaccg ctgattgctg gcgggctggt gtgcgatgaa gaagatgcgc gtaatgcgat
     7621 taacgcgggt gttgtggcgc tttccaccac gaataccggg gtctggacgt tagcgaaaaa
     7681 attgctttga cgggatactc gtaacgagtt gaatttgtgt gatttgtagg ctgcacgcca
     7741 catccgacat tcagcgcctg atacgacgct tgacgcgtct tatcaggctt acaagtcccc
     7801 agcccagaac cgtaggcagg atcatccggc agcacgttat tggcaatctg cgtgaatggt
     7861 tgtcaccagt gcctccagca ccgctttcca gtcatcaacc acgccaacat ctgcctgtga
     7921 aaataccgcc gcgctggcgt catgattaat cgccaccaca aacttgctat tgcgtacgcc
     7981 cgccattaac gctgctgcgc cggaagcgcc aacaacaata cacacctcag gtgccagcag
     8041 atgtccggaa ataccaatca ctttttcggc atcaacgcca ccgttcatca ctcgtgcccg
     8101 actgtagccc acttcggctc ccatcttttc tgccagcata gcgatttcct ggttatctgc
     8161 ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt cacgggtaac
     8221 atttttcaga ttttcgacgc tgataagcca gtctggaagc gcgccgggga caatgttcag
     8281 ttgctgcatc ccagagggca acgtggcgtt tttatctgct cccgcctgac gtgccagcga
     8341 aaggcatagc gggcgttttt ctgtttgcaa cgtcgccgtt agcgcatttc cccagtgaga
     8401 tttgcgcacg cttgcggttg atatatcgaa tgaaatcacc tggcagatac tggcgccatg
     8461 tacacgccag gccagtcggg tagccagttc atcgccaaac gcgcccggag gaaagaggac
     8521 aacctccgca ggcgttcgtt gccactgttc caccagcgca tccagcacct gttccgcaat
     8581 catcggctgt ggttctattt gccagtgtgc cagggtacaa ccgctgaaat cctgcgccgc
     8641 cagccagctc gctattgcgg catggtcctg atttattgtc acaattgcga tattcatggc
     8701 tgcatccttt ggcgtaaata gtcctgccat agcttttgcg ctttttctgc taccgtttgc
     8761 ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag acactgcatc
     8821 gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc cgccattcgc
     8881 tgacgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc aatcaccgca
     8941 ggcaagcgaa cccggcaaca gcgcagcccg ttttcagtac gctgttcaag ggtgataaac
     9001 ggcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccaa catttccgcc
     9061 agtaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccagt aattatcagc
     9121 tccagcggat tctggtactg ccattcggca atatggcgag cgacaaattc cggcgcaaag
     9181 cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc cataagatag
     9241 cgcagccagt gcaacgcccg ttcatccccc atgctcaacg cggttaaaga catcgacgta
     9301 ccgttttttc tctgcgtcaa cagcagcgcg gcggcagcct gttcatcagc accgagtaaa
     9361 cttcgcagaa gcgaaacatc cggtccgcta ttaccctgag ccgccgcctg ccactctttt
     9421 tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt cattcgcacc
     9481 tcctgcattt cccgccgcca ccagcgggag tgctttggtt tctggtgccc atagccaggt
     9541 cacgacaaaa ccaaccaaca acactgccgc caggagcaat aaggtgactt gcattcccca
     9601 ctgcgccagc acccacggca gcaggccagt acttactgcc gcgcccaacc gactcatggc
     9661 ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg caggcaaaat
     9721 gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa gcagcagcgt
     9781 taatgaactg ccggaaggca aacaggccat tactaccagc gttgccgcca gcagcaaaaa
     9841 acttcccagc aaaaacttgc gatgtgccag caggtgcgtc agaactaatc ccagcagcgc
     9901 gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt ccagaccaat
     9961 agtttgggcg atggtcggca gccaggtata aatcacaaac catgggatta cgaggcagac
    10021 aaagaagacg ctgttaaacg ccgtgcgccg ccagtagcgc gaagagaaca aggttttgat
    10081 gtgtttatgg gtcgccgttg ccacttcatc gcccagtaaa acatgcgggc caaaatagcg
    10141 atgaacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc gtggcgattc
    10201 cggcgtgccc caacgtagca gcgtaatcag caaggctggt aacgccgctg atgccagcag
    10261 ccagcgccag gcctccgggc tttcggaaat aaagtgatgt ccggcaatac ttgccagcac
    10321 atagcctacg gtccacacca cactgaatgc gcccagcaaa ataccgcgat ggcggcgcgg
    10381 ggaaaattca gccagcaagg tgtgaccgac tgagtaatcg cctcccagcc caatgccgat
    10441 caaaatgcgc aatccaataa gatgctctgg cgtggtggca aaaaactgta aaaacgaagc
    10501 aagcgtaatc agcataaaac tgaaggtgaa gattttttgc cgcccgatat ggtcggagat
    10561 ccaccccaaa accaggctgc caagaaacaa accaagtaac gccgagccgc cgatcattcc
    10621 cgctataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt aaccaataac
    10681 gccgaggaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa ttcgacagtg
    10741 aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct ttaccccttc
    10801 attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac gccccgtcat
    10861 cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg tgttcataat
    10921 gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc
    10981 cagtttgctc cagtgaacgc gatgtttacc aataccgtgg tggtgcacca tcgaaccgcc
    11041 gaggcgaatg gtttcttcgc aaatgatttt gttgagcgga ttgtggtact tgtcgatttc
    11101 ctcttccggc ttacagtcaa cgacgttgta gtcgtagacg aagtacatgt tggtgccgtt
    11161 ctggtagctg tgagaggagt gaccgcccag catggtgatg tcatcggcgt acgggaactc
    11221 ggtacgaata cggttaataa cgctttcgta gatttcgtga atgcagctcc agcagccgga
    11281 cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac
    11341 tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg
    11401 ctggcactgc gggtagcgcg ccacgatttc agcaatccct tcacccgtcg ccttcgcaat
    11461 gcgagggtta ccttcagcca taaagatcag cacgcatttg ccgtcggcaa aatgggtgaa
    11521 gtgttgggtg ccatcttcag catcatacaa acgagcaatc gacggacgat acccttccac
    11581 catgatttca cgcaggatgt tgaagccggt tttcatgtct tccagcacat agccatagaa
    11641 gagattgttt tccggcgtaa atttgaagat tttcactgtg acttcagtga tatagcacag
    11701 tgcaccttcg ttgccgatga tgatatgacg aatgtccggg ccagccgcgc gacgtggcac
    11761 gtttttaatg cgtgtgacgg tgccatctgc cagcaccgct tccagaccaa cgaccatatc
    11821 ctcgattgcg ccgtagagtg tggaaaactg cccgatactg cgggtcgcca ccaggccgcc
    11881 catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa
    11941 cgcgttttcc agcacctcca gcggaacgcc acattgcgcc gtggcctgca tattctcaat
    12001 atcaatatta atgatttgat tcatggcgga gccgtcgagc accaccgagt tttccacaac
    12061 ggtttccagc ccaccttcgg tggcggaagc accggtacgt ggcacaccgt taattttgtg
    12121 cgcattcata aaattcagca cacgggatac ttgttctgta gaaccgagtt ttacgaccgc
    12181 tgccggaatc ggcaaagtat aaattccatg aatatccgga aatttacgaa aacggtcaat
    12241 actgtttttc tttaataccg tttcatcggt aattacgcga tctgcaccaa caatttcctt
    12301 tagctggtcg acaatcgcta cgcgagataa agacatagta attccttcct gataataaaa
    12361 atggaatagt tattaataag agaaaacaga aaccaccgtt atgaattagc gcactaaata
    12421 accgccatca accactaata aatgtccgtt gacataattc gatgccggac ttgcgaggaa
    12481 tacagctgca cccattaaat cctgagtatc accccaacgg tttgccggaa tatgatcaag
    12541 aacacgctgg ttagtttctg gattactacg ggtcgccagc gtaatatcgg ttgcataata
    12601 accaggagca ataccattca cctgaatatt atattggcct aattcatcgc aataggcttt
    12661 ggtgaatccg gcaagagcat gtttagtggc agaataagca ggtgaccatt gaccacctaa
    12721 gtaagagaac aatgaacaga tattaatgat tttaccgctt ttctgcggga tcataatttt
    12781 tgccgcttcg tagcttaact cgaatgcggc ggtcaggttc acgtcaatca tcgggtccca
    12841 gtcggcacga ccaaagtcca gcaccttatt cagcttacaa ataccggcat tgttaaccag
    12901 aatatctacg gtaccgaagc gctcacagca ggcagcgata atcttctgcg gcgcgccttc
    12961 tgcggtgata tccacctgca taaaatcaac ttcgacaccc tgtttttcaa tcatttcctt
    13021 agtttcgccg ttatctttga cgaaactagg aataaagaca tttgcgccag ctttggccaa
    13081 cgccatggca aatgcctggc ctaaaccgct attcccgccg gtaacaattg ctgttttacc
    13141 tttcagggag aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc
    13201 caatttccgt aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt
    13261 tctcattatc tctggtaact ggagataatt tttagcataa gaacataact gctaatgtga
    13321 tcgtaatcac agtgtgatgt tagtattgtc aaataattta aaaaagcaga agcgtgatta
    13381 ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag
    13441 aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg
    13501 gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt
    13561 agcggcggcg ttagtttcga cctagcttat ttacgttata tttatcaaat tcccatggca
    13621 aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg
    13681 gccattattt tctatgcccc tagcggcgtt attgccgata aattttcaca tcgcaaaatg
    13741 attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac atatccaccg
    13801 ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg
    13861 tcggtgtcga ttaaagccgc atcgttgctg ggcgatcata gcgagcaagg gaaaattatg
    13921 ggctggatgg aagggatgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg
    13981 gtcttttctc gctttgcacc ggatgacagc gccagcctga aaacggtcat tatcatctac
    14041 agtgtggttt acattctgct gggcattttg tgttggtttt ttgtcagcga taacaacaac
    14101 ctgcgcaata ccaataacga agaaaaacag tcattccagc ttagcgacat cctggccgtt
    14161 ttgcgcatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt caccatctac
    14221 gccattctga gttactccac caactatctg accgaaatgt acggcatgtc actggtggcg
    14281 gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttggc
    14341 ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtggtcca aatcctttcc
    14401 atcatcggtt tgctggcgtt gacagccctg ctcgtcacga actctaaccc gcaatcggtc
    14461 gcgatgggga ttggcctgat tttattactg ggattcacct gttacgcctc acgcgggctg
    14521 tactgggcct gtcctggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt
    14581 atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat cggctactgg
    14641 caagacaccc tgcccgctgc agaagcctac cgcaacatgt ggctaatggg catggcggcg
    14701 cttggcatgg tgatcatctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc
    14761 gcccccgcaa tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga
    14821 aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc
    14881 tggaaggtaa tgtggtttgc gaaggcaaag gcttattgca gccgatgcac acgccagatg
    14941 ccgataccgc agaacatcct gacgacgatt tatgggcatc attatgtttt gccggtcacg
    15001 atttgatgag tcagtttgcc ggaaataaag aagatattgt cggtattggt ctgggatcca
    15061 tccgttgctg ccgtgcgtta ttgaaagccg atggcacgcc agctgcgccg ttgattagct
    15121 ggcaggatgc acgcgttaca cgcccttacg aacatacgaa tcctgacgtg gcatatgtca
    15181 cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac aatatcgcca
    15241 actattttgg tcagtggccg gtggattata agacctgggc atggagtgaa gatgccgcag
    15301 tcatggagaa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg ccgggcactg
    15361 ttcttgggca tatcactcct caagctgcgc tggcgacaca tttcccggct gggctgccgg
    15421 ttgtttgtac caccagtgat aaaccggtag aagctctggg agccgggtta ctggatgatg
    15481 aaacggcggt aatttctctt ggcacctata tcgcgttgat gatgaacggc aaagcactgc
    15541 cgaaagatcc ggtcgcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg
    15601 aaggttacgg tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg
    15661 agtcgttaat tcaggatgcc aaagcacagg atctttcacc ggaagatttg ctcaacaaaa
    15721 aagcgtcttg cgtgccacct ggttgtaatg ggctgatgac ggtgctggac tggctgacca
    15781 atccgtggga accgtacaaa cgcggaatta tgatcggctt tgattccagt atggattacg
    15841 catggatata tcgttcgata ctggaaagcg tggcgctgac gctgaagaat aattacgaca
    15901 atatgtgtca tgaaatgaat cactttgcaa agcatgtgat cattactggc ggcggttcga
    15961 acagcgatct gtttatgcag atttttgccg acgtgttcaa ccttccgaca cgccgaaacg
    16021 ccattaacgg ttgtgcaagt ctgggggctg cgatcaatac ggcggtaggt ctggggccat
    16081 acccggatta cacaaaggct gtcgataaaa tggttcgcgt gaaagatatc ttcataccga
    16141 ttgagagcaa tgccaaacgc tacgacgcga tgaataaaag cattttcaaa gacctaacca
    16201 aacatactga tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatg
    16261 cggattcgat ccagagctgg tcgaatgcgt aagtgagata aacagtgccg ggtagatgat
    16321 taatcccggc atattcacgt ttccactaca aaataccggg taaccactgc tgaatattgt
    16381 tctctcctga gcagcgttta tgctttaatt gctaaaccga tttaatttgt ttagcagtta
    16441 gtatattgga gcctttatga gcacaagagt atgggtactc ggtgatgcgg ttgttgattt
    16501 attacccgaa agccagggga gactactaca gtgtcctggt ggggcgcctg ctaatgttgc
    16561 agtcggtatc gcaaggctgg ggggggaaag tgcctttatt ggcaaagttg gcgatgatcc
    16621 tttcggtcgc tttatgtatc agacactgag taaagaaaat gttgatacac gttatatgtc
    16681 tcttgatcct caacatcgca cctcaattgt ggctgtagga cttgatgagc aaggagaaag
    16741 aaactttacc tttatggtac gcccaagtgc cgatcttttt ttacaacctg atgaccttcc
    16801 tacatttggg ccgggtgaat ggctccatct ttgttccatt gcgctcagtg cagaaccttc
    16861 ccgaagtacc gcatttctgg ctatggagaa aatacgtcag gctggcggaa acatcagttt
    16921 tgatcccaat atccgcagcg atctctggca gagtgaagcg ctattaagaa aataccttga
    16981 tcgcgcgctt tcgctggcga atatcgctaa attgtccgaa gaagagttgc tattcatcag
    17041 tggcgaaagc caggttcagc aaggcgcata ttcattagta caacgttatt cgttgacttt
    17101 attgcttatc acacaaggaa aaaatggcgt acttgtgtat tttcaggggc agtttatcca
    17161 ctatcccgcc aaacctgttt ctgtcgtcga tacgaccggg gcaggagatg cttttgtcgc
    17221 tggattactt gccggtctgg ctgattctgg aataccaaca aataccagac agcttgaacg
    17281 aatcattgca caagctcaga tttgtggtgc tctggcgacc acggctaaag gcgcgatgac
    17341 cgccttaccc cgacaacacg atctcccttc acaatagtga tcctgctgtt gtatctgtaa
    17401 cccgatttgt cgtgcctgtc acatttacta aatcggttta gcgaaaataa ttgctctggc
    17461 taaaaagtca gctttattat caatgcctaa accggtttag cattttagca atttttataa
    17521 ttctctgatg ggacgcgaca aattatgtat aaaaaaacaa ctttggcagt gttaattgct
    17581 ttgctgaccg gtgctacaac ggtacatgcg caaacggata ttagcagtat cgaatctcga
    17641 ctggcggcgt tggaacaacg tttaaaaaat gcggaatccc gcgcccaggc agcagaagca
    17701 agggccaaaa cagctgaatt acaggttcag aaactggctg aaacacaaca acaaaatcag
    17761 ctaacaactc aagaagtagc acagagaaca gttcagctcg aacagaaatc cgcagaaaac
    17821 agtggttttg agtttcatgg ctatgcccgt tccgggttac tgatgaatga tgccgcttcc
    17881 agtagcaaaa gtgggccgta tctgactccc gcaggtgaaa ctggtggagc tgttggccgt
    17941 ctgggaaatg aagccgatac ctatgtcgag ttaaatgtag aacataaaca aacactggat
    18001 aacggtgcga ccacacgctt taaagcaatg ttggctgacg gacaaagaga ttacaacgac
    18061 tggactggcg gctccagtaa cctgaatatc cgacaggctt ttgccgaact gggcgcatta
    18121 ccaagtttta ccggagcatt caaagacagt actgtctggg ctggtaaacg ctttgatcgc
    18181 gacaattttg atattcactg gttagactcc gatgtcgtat ttttagcggg aacgggcggc
    18241 ggtatctatg acgtaaaatg gaacgataca ttccgcagta acttttctct ctacggacgt
    18301 aatttcggcg atcttgatga tatcgacaat aacgttcaga actacatcct caccatgaat
    18361 cattatgcag gccccttcca gttgatggtt agcggattac gggcaaaaga taatgatgat
    18421 cgaaaagatg ccaatggtga tctcattcaa actgatgctg caaatactgg cgtacatgcg
    18481 ttagttggtc tgcacaatga cactttctat ggcctgcgtg aagggacggc aaaaacagca
    18541 ctgctatatg gccatggcct gggtgcggaa gtcaaaggga ttggctccga tggcgctctg
    18601 ctgtctgagg cgaatacctg gcgcttcgca tcttacggca caacacctct gggaagcggt
    18661 tggtatgttg cgccagcaat tctcgcacaa agcagtaaag atcgttacgt caaaggcgat
    18721 agctacgaat gggtgacctt caatacacgt ctgatcaaag aggtaacaca gaattttgct
    18781 ctggcctttg agggtagcta tcaatatatg gatctgaagc caaaggggta tcaaaaccac
    18841 aacgccgtaa acggcagctt ctataaactc acctttgctc caactctaaa agctaacgat
    18901 atcaataatt tctttagccg tccggagctt cgcctgtttg ccacctggat ggactggagc
    18961 agcaaacttg atgattttgc cagcaatgac gctttcggca gcagtggttt caatactggc
    19021 ggagagtgga attttggtgt ccaaatggaa acctggtttt aataccagac agcctcacgg
    19081 atgtgaggct tttactgaaa taacaatgaa ttagagggtt atatggattt cgataaaatc
    19141 gcccaatcgt tgcttccttt attaggtggt aaggagaaca tcgccagtgc agcacattgc
    19201 gccactcgcc tacggctggt actggttgac gacacacttg ccgatcaaca tgccattggc
    19261 cagattgatg gagtgaaagg ttgttttcgt aattcagggc aaatgcaaat catctttggc
    19321 accggcgtgg ttaataaagt ctatgctgcc tttattcagg ttgcaggtat tagcgaatcc
    19381 agtaaagcag ataccgctcg actcgccgct caaaaactca atccttttca gcgaatagca
    19441 cggctacttt ctaatatttt tgtccccatc attccagcca ttgttgcttc tggtttatta
    19501 atggggctcc tgggaatggt gaaaacatat ggctgggtga atgccgataa tgcgatttat
    19561 atcctgctgg atatgtgcag ttcagccgca tttattattc tccccatcct tattggcttt
    19621 actgctgctc gtgaatttgg tggaaatcct tatcttggcg cgacattagg agggattctg
    19681 actcacccgg cactcacaaa tgcctggggc gttgctgcgg gatttcagac aatgaacttc
    19741 ttcggttttg aaatcgccat gattggctat cagggaacgg ttttccccgt gcttctggca
    19801 gtatggttta tgagcatagt ggaaaaacag ttacgcaggt ttatccctga tgctctggat
    19861 ctcattctga cgccatttct gactgtcgtc atttctggct ttatcgctct tttgattatt
    19921 ggtccggcag gacgagcgtt aggtgacggt atctctttcg ttctcagcac gctgattgca
    19981 cacgcaggct ggttagcagg gttgttattc ggcgggctat attcagcgat tgttattacg
    20041 ggtattcatc atagcttcca cgcaattgaa gcaggacttt taggtaaccc tgcaatcggt
    20101 gttaatttcc tgctgcctat ttgggcaatg gcaaacgttg cgcaaggcgg tgcatgtctg
    20161 gcggtatggt ttaaaaccaa agatacaaaa attaaagcaa tcaccctacc ctcggccttt
    20221 tccgcaatgt tagggattac tgaagccgct atttttggta tcaaccttcg ttttgttaaa
    20281 ccttttattg cggccttaat tggtggcgct gccggcggtg cctgggttgt ctctgttcac
    20341 gtttatatga ctgccgttgg tctgaccgca atacccggca tggcaatcgt tcagccaaca
    20401 tcgttggtta attatattat tggaatggtg attgctttcg ctgtcgcttt tagcctctct
    20461 ttattactca aatacaaaac ggacgaggag taatgtcgat gccttcacaa atgcccgcga
    20521 ttttacaggc agtgatgaaa ggtcagtcca aagcactggc agatgcccac tatccttgtt
    20581 ggcatctcgc tccagtcacc ggactaatga acgatccaaa tggcttttgt tggtctggag
    20641 gccgctatca tctgttttat cagtggaatc cactggcttg tgatcataag tacaaatgtt
    20701 gggggcactg gagttcgacg gatttattgc actggcagca tgaaccatta gcgctaatgc
    20761 ccgataaaga gtacgaccgt aatggttgtt actcaggaag cgcggttaat aatcagggcg
    20821 ttctcacact ttgctatacc ggtaatgtta agtttgatga tggcagcaga acggcatggc
    20881 aatgcctggc caccgaaaat aaccagggcg ggtttgataa actaggccct gtcataccgt
    20941 tacctgatgg ttacaccgga catgttcgtg atccaaaagt ctggaagcac aacagccaat
    21001 ggtacatggt gcttggcgcg caagataaag agaaacgggg gaaagtgctg ctgtattcct
    21061 ctgtcgatct ctatacctgg tcttttcatg gagaaattgc tggtaacggt ctgaatgaaa
    21121 tagataacgc aggatacatg tgggaatgtc ctgatttttt cgcccttgat ggtgaataca
    21181 ttctcctctg ttgcccccag ggaatggtgc gcgaacatga gcgctatctg aatacttatc
    21241 cctgcgcctg gctacatgga cagtttgatt acgagacagg caaatttacg catggcgctt
    21301 tctcagagct ggatgcggga tttgaattct atgcaccaca gacgatggaa gccccggacg
    21361 ggcgacgatt gctggtcggc tggatggggg ttcctgatgg cgaagagatg ttgcagccaa
    21421 ccagaaagca tcattggcag catcaaatga cctgttttcg cgaactatca ttccagcagg
    21481 gaaaattgtt tcagatgcca atcagggaac tcgcgcaact gcgtgaagct gaacacttct
    21541 ggcaaggaaa agcggatcat gcgccgcatg tcgaaattga acgcctggaa atggacatca
    21601 ttccgtcggg tgagctgtat ctgaattttg gcaatgcgct ggcactgcat cttaatgacg
    21661 atggtattca actacagagg agaagccttg cagggcaaga gaaactaact cgttactggc
    21721 gaggaagcgt aacgtcactg aagattctct gtgacagttc cagcgtagag atattcatca
    21781 ataatggtga aggtgtaatg agtaatcgtt attttcccca tcatccagct tcgctgatat
    21841 tacagggtga gtctgacgtc acgttacgct actggtcgct acgcgcctgc atggtagaat
    21901 gagggttttg aattacggat cctcgtcgtg agaaaaacca aacgcgttac tattaaagac
    21961 attgctgaac tggcgggagt gtcgaaagca accgccagtc tggttctcaa tggtcgtggc
    22021 aaagagttac gtgttgcgca ggaaacgcgt gagcgtgtgc tgagtattgc ccaacagcag
    22081 cattatcagc ccagcattca tgcccgctca ttacgggaca accgcagtca taccattggt
    22141 ctcgtcgttc ctgaaatcac taactacgga tttgcagtat tttcccatga actggaaaca
    22201 ttgtgtcggg aagcgggcgt ccagctcctc atatcctgta ctgatgaaaa tccttcacag
    22261 gaaacaatgg tggtgaacaa tatgattgcc cgccaggttg atgggttaat cgtcgcatcc
    22321 agtatgctgc atgataatga ctatcaaaaa cttagcgagc aattacctgt tgtgttgttc
    22381 gatcgccaca tgaacggcag tacattgcca ttggtcatta ctgactctgt ttctcccacc
    22441 gccgctctgg tggcagatat tgcaaggagt cacccggatg aattctattt tctgggcgga
    22501 caaccccgtt tgtcaccgac tcgcgatcga ctggaaggat ttacacaagg cttgcagcaa
    22561 gccggagtaa cgctacaacc tgaatggatt attcacggta attatcatcc gagctcaggc
    22621 tatgaaatgt ttgccgcgct atgcgctcgt ttaggacgtc cccctaaagc cttattcact
    22681 gccgcttgtg gattactgga aggcgttctg cgctatatga gccaatataa tttactcgac
    22741 agtaaaatac acctggcgag ttttgatgat cattacttgt atgattcact ttcggtaaga
    22801 atcgacacaa tacaacagga taatcgccaa cttgcttttc actgctttga acttatttcg
    22861 caactgattg aaggtgaaac gccatcccca ttacagcgtt atcttcctgc cagtctacaa
    22921 aaacgctacc gataatcccg tcactatctt gcatcgcccc tgtaaaacgg ggcgattatt
    22981 ttcatcctga acgttaaata ttaatgatga ataatcaaat caggcaatat tcaaatattt
    23041 atgtgtctgc atcgacaaac gccagttgcg cgcaatgcag gtatcgatac ataaacgcgt
    23101 agcatcttcc ttctggctaa ttggctgaag tgcaatgact cgcggtttat catcggtcag
    23161 cgtcgccagc aattcatcca gtgcttcaat atcacgtacg cgtcctactg ggtgtttgat
    23221 ttcgttggct cgctccaggg cttgtgacaa cacttcatag ccaccgcgca tatttagctt
    23281 tggtgatacg gtaacccagg tattcggtgt gcagcgtact tcatgagtac cactggtttc
    23341 gatctggcag ctaaaaccgt tcttttcgag caggtcagtc agtggcagca aatcatgaat
    23401 gcaaggctca ccacccgtaa tcaccacatg acgcgcggtg tatccctgac ggccaatgac
    23461 agccagcaaa tcttcactgc tcgccgcccc ccacttatca ctctctttgg tcttcgccag
    23521 aatgctgaaa agggagactt cccgatcctc aagcttttcc caggtgtgtt tggtgtcgca
    23581 ccaggcacag ccaaccggac atccctgtaa acgaataaaa atggcgggga cgccggtaaa
    23641 gtaaccctca ccttgcaggg tctggaacat ctcgttaatc gggtactgca tagcattctc
    23701 tgtgaagtgg ataattgtta attattgcag atcctgccac aacaatcatg tcttattaac
    23761 attctgttac aggcaggttt gatgcaaaaa ttggctcaga tcattaagaa taaaatgaag
    23821 cggcgtatta tgttttaagg aattaattaa aaggaattta accatgctca gaatattcat
    23881 cgcaattata tttattttca atttatcagg ctgtggatat aacgatattc aaacctatga
    23941 tgagcaggtt aacgcctcct ggtctgaggt attgaatcaa tatcaacgtc gcaccgatct
    24001 tattcctaat cttgtcgcca gcattaaagg ttattccagc catgaacgag atgtactgga
    24061 agccgtaaca ttggctcgta gccaggctaa tcgcgccagt agcgatcttc agcaaactcc
    24121 gggagatgaa caaaaattac aaacctggca acaagctcag gcgcaggtgt ctcgcactct
    24181 cggtcagtta acgataatta gtgaacgtta ccctgagcta aaggcccagg aactctatca
    24241 aaatttgatg gtacagctcg aaggaagtga aaatcgaatt gccgttgcgc gaggaagata
    24301 tattaaagct attgaacaat ataatgtcac tatccgcaaa ttccccgcag tgttaaccgc
    24361 aaaggtcatg gattacacac caaagaaaaa ttatttgccg gatgatgtcg ccgctgtgag
    24421 taaagccccg acaatagatt ttagccagaa tactaatgct cattaagcgg ggaagtcctg
    24481 tgcgaatttt tatcttcctt atggctttct tctgttcatc tgtgtttgcc acacaaattc
    24541 cagtgcctga atcacccagg tatgtgaatg acttaaccgg taaattgacg aacgatgaag
    24601 taaatacttt gaccaatcag attaaagagc tgacgcaaaa aagccacgca caactggtcg
    24661 tgttagtggt tgaaacaacg ggcgatgaaa ccatcgagca gtacgctacg cgggtattcg
    24721 atagctggaa accaggtgat aaagaccgcg atgatggcgt tctactgtta gtcgcctggc
    24781 aggatcatac cgtgcgtatt gaaatcggtt acggactgga aggcgttatt acggatgcgc
    24841 agtccgggaa aattattcgc aacagtatta ttccggcatt taaaaatggc gacctggctg
    24901 gcggcttgca aaagggaata agcgatattg ataatcgtct gacggggaat aatttagcaa
    24961 cgataacacc gaccgatcac cctttgccct ttaacggttg gtgggctttg ttagtctggg
    25021 caatagtact gacctttatt tccgccagag gatatttcaa aacgctggga ctaatttgct
    25081 tcacagctat tgtactggcc tttgttttac ctatagcagg tttcagcgga agctggggag
    25141 tactcgcaac attactctgc ttcgctacac cgttcctggc tgtcgctatc gcttttaccc
    25201 ccttcggcaa aaaggttcgg gattcgatgc gtagtgccaa ccgaccgtca cgttacgaaa
    25261 gaagtaactc ggcctcctgg cgttcttcat catcgtcgtc atcttcctct tcatcctctt
    25321 ttgacaatga taatttcagc ggcggcggtt catctggcgg cggtggcgcc tccgggcgct
    25381 ggtaacaaaa ggaattgaaa tgcgtatttt tctcttactt ctgatgctat atggtttcca
    25441 ggcattagct gaacaattgc cttcagtaac tgacttaacc gggacattaa cgacagaaga
    25501 acaaactgct ttaaatcaac aattacagac tctcgagcag caaaaccact tccaggtggc
    25561 cgtgttggtt acgccgacca cagggggcaa aaacattaaa cttgcagcct ctcagagcta
    25621 cgggttatat cactggaaac ccgttggaga gaagcggtac ggcgaaggta tcttaattct
    25681 cgtcgtttgg ccggaagggc tggcgtcaat gaagattggg catggactga aagagatgct
    25741 gccaccggaa caagctgcgc aaattgttca ttaccatatg caaccggaat tcaaaaaaaa
    25801 taatctcttc tccgggttaa cgcagggaat tgaaggcatc gcacagttta cctatataaa
    25861 agcgaatctt agccctttgg atgctttagc caatcatctg tttgctaatc cgcaacgctc
    25921 gctgccatgt cttgcctgga cattactgat gatcgttgcc atcgttgtcc tctggcgatt
    25981 taccagccgt cccgggcctg gcatatggat gatatcgatg attactccta tggtctggtt
    26041 tttcttcttt caggatgatg tcatcattcg gcgggtatcg ctcgtttgtt ttagtttatt
    26101 tttcgcagca acctgctggc agcgtatcgc ggtaattttt catatgtatc gcgtatttcc
    26161 tatgatgctg gccccaaaaa ataaaaacgc taaagtgaaa aaacaaaaga cgactcagca
    26221 ggtttctcaa tccgaacgaa actcattgtt tgtcgtgctg ctgataattg ttgtgggatg
    26281 gtgcctggta ttcgccagta ataaagatat agcattcatc tcaacaatta ttgggctaat
    26341 tgatcatttt atcggcccaa taaccattgc tggcatcgtt ctgctgatca ttgtggccaa
    26401 atttactggc aatcttaaac tgggatcagg acataaaagc aacaggaaaa cggggaatcg
    26461 taatgctcat tctcgctctt cgtcgaaaaa ttctttccgc ggtggcggcg gttcttcagg
    26521 aggcggcggt tcatccgacc gttggtgatc gttatgcgta taatcctttt tctactcact
    26581 ttatggtgcc ttcctgtgct tgcccagcaa attgctgtgc cggagctgcg ccaacaggtg
    26641 accgatatta ctggtacctt aagcacgtct gaacaacaat cactaacaca acagctgcag
    26701 gatattacac aaaaaacacg ggcacaggtc gctgtattaa tagtgccatc caccggagac
    26761 gattctattg aacaatatgc tacaagagtt tttgacaatt ggcgtttagg agatgccaaa
    26821 cgcaatgatg ggatactgat cattgttgcc tggtcggatc gcactgtccg catccaggta
    26881 ggttatgggc tggaagaaaa ggtaaccgac gctctggctg gagatatcat ccgtagcaac
    26941 atgatacccg catttaaaca acaaaaatta gctaagggat tagagttagc tataatcgct
    27001 ttgaataacc gactcacttc acaacaccaa tattcggtta atccttcaga aagtgaatca
    27061 gcatcttcca gcgatcatta ctactttgct attttttggg tatttgcagt gatgttcttc
    27121 cctttctggt tttttcatca aggcagtaat ttttgtcgcg catgtaaaag cagtgtctgt
    27181 atttcagcaa tctatctttt agatttattc ctgttctccg ataaaacttt ttccattgct
    27241 gtatttttct tcttttttac tttcaccacc attatggtct ttacctgttt atgtgtactt
    27301 cagaaaaaag catctggtag aagttatcat tcagacaaca gcggttccgg tggtggctca
    27361 agtggcggcg gatttagcgg cgggggcggt tcttctggcg gtggcggcgc gtccggtcgc
    27421 tggtaactca accataaaaa atgccagccc gaaggctggc atttttaaat cagataaagt
    27481 cagacttatg cctggccttt gatctcttta cgaccgttgt acggtgcttt ttcgcccaga
    27541 gcttcttcga tacgaatcag ctggttgtat ttagcaacac ggtcagaacg gctcatagaa
    27601 ccagttttga tctggcctgc agcagtacca acagccaggt cagcgatggt agcgtcttca
    27661 gtttcgccag aacggtgaga gataactgca gtgtagccag cgtctttcgc catcttgatt
    27721 gcagccagag tttcagtcag agaaccgatc tggttgaatt tgatcaggat ggagttagcg
    27781 ataccttttt cgataccttc tttcaggatc ttggtgttgg ttacgaacag gtcgtcacca
    27841 accagctgga ttttgtcgcc cagaactttg gtctggtatg cgaaaccgtc ccagtcagat
    27901 tcgtccagac cgtcttcgat ggaaacgatc ggatactgtt tggtcagttc ttccaggaag
    27961 tgagtgaatt cttcagaggt gaacgctttg ttgccttcgc cagccagaac gtatttaccg
    28021 tctttgtaga attcagaagc tgcgcagtcc atcgccagag tgatgtcttt gcccagttca
    28081 taaccagcag ctttaacagc ttcagcgata acagccagag cttcagcgtt ggaacccagg
    28141 ttcggcgcat agccaccttc gtcaccaaca gcagtgttca tgcctttcgc tttcagaact
    28201 tttgccaggt gatggaaaac ttcagaaccc atgcggatgg cttctttcac agttttcgcg
    28261 ccaaccggct gaatcatgaa ttcctggata tcaacgttgt tgtcagcgtg ctcaccaccg
    28321 ttgatgatgt tcatcatcgg aaccggcata gagtatttgc ccggagtgcc gttcagttca
    28381 gcgatgtgct cgtacagcgg cataccttta gcagctgcag cagctttggc gttagccaga
    28441 gatacagcca ggattgcgtt cgcgccgaat ttggatttgt tttcggtgcc gtccaggtcg
    28501 atcatgatct tgtcaatgcc agcctgatct ttagcatctt tgccaatcag cgcctgagcg
    28561 atcgggccgt ttaccgcagc aacagctttg gttacgcctt tacccaggaa acgggatttg
    28621 tcgccatcgc gcagttccag agcttcacgg gaaccagtag aagcacctga cggagcagct
    28681 gccataccga cgaaaccacc ctccagatgt acttcggctt caacagtcgg gttaccacgg
    28741 gagtcgatga tttcacgacc gatgattttt acgattttgg acattaggtt ttcctcaagt
    28801 cactagttaa actgaaactc cagacaaaca acgcgtaccc agggtacgcg ttgccgttct
    28861 aactttttta cttacttcgc ctgacgtttc tggaactcgc tggcggcttt cacaaagcct
    28921 gcaaacagcg ggtgaccatc acgtggagta gaagtaaact ccggatggaa ctggcaagcc
    28981 acgaaccacg gatgattcgg aacttcgatg atctcgacca actgatcatc cccggaacgg
    29041 cccgcaacgc gcagacctgc atcttcaatc tgtttcaaca gcatgttgtt gacttcgtaa
    29101 cggtgacgat gacgctcaac aattgtcggc gcattgtaca gctggcgaac caggctatcg
    29161 tcaaccaact ggcactgctg tgcgccgaga cgcatggtac cgccgagatc gctcttctcg
    29221 ctacgaactt caacgttgcc gttttcatcg cgccactcgg taatcagcgc cacaaccggg
    29281 tacttacagt ctggcacaaa ttccgtagag ttggcgttct ccatgttggc aacatggcga
    29341 gcgtaatcaa ttaacgccac ctgcataccc aggcaaatgc ccagataagg aatattgttc
    29401 tcacgcgcaa aacgcgcggt cgtaatcatg ccttctacgc cacgatagcc gaaaccgcca
    29461 ggtacgagga ttgcgtccag acctttgagg atttcaacgc cgcgcgtttc aacatcttgt
    29521 gaatcgatca gtttgatgtt gacgctgaca cgattcttca gcccaccgtg tttcagtgct
    29581 tcgatcacgg atttgtatgc atccggcagc tcaatgtatt tgccgaccat accaatggtg
    29641 acttcactta ccggattcgc ttcttcgaag ataacctgtt cccattcgga cagattcgct
    29701 tccgggcagt ttaagctgaa tcgtttacaa atataatcgt ccagcccctg agatttcaac
    29761 aggcccggaa ttttatagat ggaatcgacg tctttcagag aaataaccgc tttttccgga
    29821 acattacaga acaatgcaat cttcgcacgt tcgttcgccg gaacggcgcg atctgaacga
    29881 caaatcagga tatcaggctg gataccgatt gaaagcagct cttttacaga gtgctgagtc
    29941 ggtttggttt tgacttcacc agacgctgcc atgtacggca ccagcgtcag gtgcataaac
    30001 agggtgtgct cacggccaat ttcaacagcc atctggcgaa tcgcttcgag gaacggcagg
    30061 gattcgatat cacctacagt accgccgatt tcgaccagta ctacgtcatg accttcgccg
    30121 ccttccagca cgcgctcttt gatagcgtta gtgatgtgcg gaataacctg tacggttgcg
    30181 ccgagatagt caccgcggcg ttctttacgc agaacgtcag agtagatacg ccccgtggtg
    30241 aagttgttgc ggcggctcat tttggtacga atgaaacgct cgtagtgccc caggtccagg
    30301 tcggtttcag cgccgtcttc agtaacgaac acttccccgt gttggattgg gctcatagta
    30361 ccgggatcga cgttgatgta cggatccagt ttcatgatgg tcacattgag gccacgggct
    30421 tcaagaatgg ctgcgaggga ggctgcggca atgcctttac ccagagagga tacgaccccg
    30481 ccggtcacaa aaatatagtt cgttgtcatg ctgaacctga gaagttaggt tgaaaagacg
    30541 atggaataac caggacggga aagcagtata cccgaacatg acctgtgcca caaactttca
    30601 ttatccctcc tcttcgccag cgcactattg aaatcaggag tgagaaaata gcccctttgg
    30661 ggtaaatgtt tttgacgcaa atcaagcgct tgtcatttaa aaaatgacac aaatcgcgct
    30721 tgaccgcgta attcccttag agatcaattt cctgccgttt tacctgttgc cagacttctt
    30781 ccattgtttc gaggtcaaca cctgtcattt ccagtccacg cgcggcaaca atacgctcca
    30841 cttcgcgaaa acgacgctcg aatttttcgt tcgctttttg caatgcgatt tccgcttttg
    30901 tccctaaatg acgagccaga ttgaccgtgg caaacagcag gtcacccatt tcctcctcca
    30961 gtttagcctg gtcgacaaca gcctgccgcg cctcgtacat cacctcgtcg atctcttcgt
    31021 agactttatc tactaccgga ccaagcgtcg tccagtcgaa accaacgttg gcgcaacgtt
    31081 tctggatttt ttgcgcacgc attaaagccg gtaaactacg aggaatatcg tccagcgccg
    31141 aatgctgcgc tttctgcgcg cgctcttcgg ttttgatttg ctcccaacgg gcaagcactt
    31201 cactactatt ttcggcagaa ctgtcagcaa aaacatgcgg atggcgacgc tctaatttat
    31261 cgctaatagc agcgcaaata tcattaaagt caaagcgccc ttcttcctga gccatttgtg
    31321 cgtaaaacac cacctggaac agcagatcgc ccagttcacc gcgcagatcg tcaaaatctt
    31381 cacgggcgat ggcgtccagc acctcgtagg tttcttcaag ggtgtaaggc gcaatggtgg
    31441 caaatgtctg ctctttatcc cacgggcagc cgttttccgg gtcgcgcagg cgctgcatga
    31501 tggtgagcaa acggtcgatt tgattcattg aattatcctg aaaattgcgg gtccgtcagg
    31561 tggaaacctg tgaccagaat agaagtgagt tagtaacacc tacccaatca gcacgttaat
    31621 tttggcttta atgagttgta attcctctgg ggcaactgtt cctttcttcg ttgctcctct
    31681 tgcccgccag gcgatacttt ttacctgatc agctaacgct acgccatcac gttcctgacc
    31741 ggatagaaca acttcgaacg gatatccttt tgattgcgtt gtacaaggaa cacacagaca
    31801 catacctgtt ttgttgttgt acatgaacgg actcaggaca acagccggac gatgtccggc
    31861 ttgctcgcta ccttttgtcg ggtcaaaatc aacccaaatc agatcgcccg tatcgggtac
    31921 gtatcggctt accattacca gacttcctta tctttcggct ccccccagtc gatattctcg
    31981 tggaggtttt ccggcgtgat gtcgttgacc agttcagcaa gcgtaaatac gggctcttta
    32041 cgcactggct caataattaa tttgccatcc accaggtcaa tcttcacttc atcatcaata
    32101 ttcagattga gcgcctgcat taacgtagcc gggatccgca ccgccggtga atttccccaa
    32161 cgctttacgc tactgtggat cataaccctt tcctcaaacc gcgatcatat gtagatacag
    32221 tatatatcaa tctacattgt agatacgagc aagtttcggc ctaactcccg tgcaaccgac
    32281 gcgcgtcgat aacatccggc acctggttga gtttacccag cacgcgaccc agcacttgca
    32341 ggttgtaaat ctcaatggtc atgtcgatgg tcgccagttg ctgtttggtg tcgctacggc
    32401 tggcaacgcc aagtacgttc accttctcgt tggcgagaat ggtcgtgata tcacgtaaca
    32461 acccactacg atcattagcc accacgcgga ccaccagcga atatccggcg gagtagctct
    32521 cgccccatac cgcgtcaaca atgcgttctg gcgcatggga gcgcagttcc gccaattgtt
    32581 cgcaatcggc gcggtgtact gaaataccgc gcccttgggt aatgaagccg acaatctcat
    32641 ctccaggaat cggctggcag cagcgcgcga tgtggtgcat caggttaccg accccttcta
    32701 ccaccacacg accgttatct ttactgcggt tttgcggcgt gtagcttttt tgctgaagct
    32761 gtttcagcgc ggcggcgtcc tgctcttcgg cactcggctt attaaattgc gattgcagga
    32821 agttcaccat ctgattgaga cggatatccc cgccaccaat cgccgccagc aactcgtcga
    32881 catcattgaa gttgtaacgc ggcagcagat gtttttctgc ttctttcagg ctgatcccca
    32941 gatgttccag ctcgtcgtca agaatttgcc gcccagccag aatgtttttg tcacggtcct
    33001 gtttacggaa ccaggcgtga attttcgaac gcccacggct ggttgtgacg taaccgaggt
    33061 ttgggtttaa ccagtcacgg ctggggttcg gctgtttctg ggtgataatt tcaatctggt
    33121 cgcccatctg cagctggtag gtgaacggca caatgcgccc gccaattttt gccccgatgc
    33181 agcggtgtcc gacatcactg tggatgtggt aagcgaagtc cagcggcgtt gatcccgcag
    33241 gcaaatcaac gacatcacct ttcggcgtaa agacgtacac tcggtcgtca aagacctgac
    33301 tacgtacttc gtcgagcatt tcgccggaat cagccatctc ttcctgccac gcaatcagtt
    33361 tacgcagcca ggcaatccgg tcttcatgtc ccgaacgtgc gccgccagca gccgcgccct
    33421 ctttatattt ccagtgcgca gcaacaccca actctgcatc ttcatgcatc tgtttggtgc
    33481 ggatttggat ctcaacggtt ttgccacccg gccccagaac cacggtatga atagactgat
    33541 aaccgtttgg tttcgggtta gcgacgtaat cgtcaaactc atccggcagg tggcgatagt
    33601 gagtgtgcac tatccccagt gcggcataac aatcctgtaa acgctcggcg acaatacgta
    33661 ccgcacgcac atcaaacagc tcatcgaagg cgaggttctt tttctgcatt ttgcgccaga
    33721 tactgtagat gtgtttcgga cggccataca cttcagcttt aacgccttca gctttcatct
    33781 cagcgcgcag atggccgaca aactcttcga tatagtgttc gcggtcgaga cggcgttcat
    33841 gcagcagttt tgcgatgcgt ttgtattcgg tcgggtggag ataacggaag cagtaatctt
    33901 ccagctccca tttcagttgc ccaatcccaa gacggttcgc caacggcgcg tagatattgg
    33961 tgcactcttt tgccgccagt acgcgttcat cttccggcgc atcttttact tcgcgcaggt
    34021 gagcaatacg ctccgccagt ttgatgacca cgcagcgaaa atcatcgacc atcgccaata
    34081 acatccggcg aacgttatcg acctgttcgg aggaaacaga atcagtgtgc gtcgctttca
    34141 gctggcggat cgccgccata tcacgcacgc cgtgaataag gttaacgacc gacttaccga
    34201 cgctctcacg cagcacatct tcgctgacta cgttggcatc agccagaggg aacagcagcg
    34261 ccgcccgcag cgtgtcaatg tccatactta atgtcgagag gatctccacc atctcaacac
    34321 cacgccacaa taacagactg gcatccggat gcccctgcgt ctgttgcaga caatacgccc
    34381 aggtttcggc taagcactca cacgacttct ggctggtaat acccagactt gcgatccatt
    34441 tttccggatc aaattcacca gccttattga tatgtgcact tcttaccgca accatcgtcc
    34501 tctcctttag ggaccagacc tgccgaaatc ggcaaatcgt aactatttaa cgcgcgagaa
    34561 aagtaccatc gattccagat gtcccgtgtg tgggaacata tccagcatcg ccagtcgcgc
    34621 aatggtatat cctgctttta ataacgcttc gctatcccga gccagcgttg cagggttaca
    34681 ggatacataa actatacgaa taggttccag ttttataatt tgctgcataa cacctgcggc
    34741 acctgctcgc gccgggtcca acaacacttt atcgaagccg tttttcgccc acggctgctt
    34801 tgtgacatct tcttcaagat tttcgtgata aaacgtcaca ttctgtaagc cattaagacg
    34861 cgcattctgc tggccttttt ccaccagcgc cggaacacct tctacaccca ccacactggc
    34921 agcttgtgtc gccaatggca gtgtaaagtt gcccataccg cagaacagat ccagtacgcg
    34981 atcttcaggt tccacatcca gccattccaa cgcacgcgct accattttct ggttcacacc
    35041 cgcattgacc tgaataaaat cgcgcgggct aaaagttaag cgcaacccat ttgagtcata
    35101 ccagggcatc tcaccagaga cggtttcgag tatctcacta tcgggggcga gatacagatc
    35161 caggccttca gaatgcgaaa agcattccag tttttcgcga tctgccgaac ttagcggtgc
    35221 ggtatggcgc aaaatcatta gcgtgccgct ggttgcctgt accagttcaa catgaccaag
    35281 atggcgcata gcttgtaagc tgcccaggca tgccctgact ttgggcagca atgcttcaag
    35341 ttggggcact aaaatggggc attgtttcac gtcgacaatg tcactggagc ccgctttgcg
    35401 aaaccccatc tgaagttgct gtgttttcgg taagtagttc agacttaaac gcgcgcgacg
    35461 gcgatagccc cagggaacat cggcgatcac ttcagagact tcgtgtttca ttaatcgggc
    35521 gagtgccgca cttttgcttc gctgctgtaa atccacactg gcgtgttgtt gctgacagcc
    35581 gccgcatacg ccaaaatgag gacagcgtgg cgtttcgcgt tccgggctat cgcttaatcg
    35641 gcgtacgact ttagcgcggg catactgttt tttatcttca gtaacagtaa cttccgcgtt
    35701 ttcctgcggc aataatccgg ggataaataa tgttttgccg ttatgtcgcg ccacaccctg
    35761 accaaaagag tcgaggtcgt tgactgaaac ggttatgatc tgacgcgtcg tcgtgcgtcg
    35821 ttttgcagag tagaattgcg ccattggcga gactttctca atttaacagt gtgaccttaa
    35881 ttgtcccata acggaactcc atgaccaact acagcctgcg cgcacgcatg atgattctga
    35941 tcctggcacc gaccgtcctt attggtttat tgctgagtat ctttttcgtc gtgcatcgct
    36001 ataacgactt gcagcgtcaa ctggaagatg ccggtgccag tattattgag ccgcttgcag
    36061 tttctactga atatggcatg agcctgcaaa atcgcgaatc tatcggtcag ttaataagcg
    36121 tactgcatcg tcgccattcc gatattgttc gcgcgatttc ggtttatgat gaaaataacc
    36181 gactctttgt cacctccaat tttcatcttg atccctcatc aatgcagctc ggcagcaacg
    36241 tgccgtttcc tcgccagctc actgtcactc gtgacggcga tattatgatc ctccgcacgc
    36301 cgattatttc tgaaagttac tcccccgacg aatcgcccag tagcgatgcc aaaaatagtc
    36361 aaaatatgct gggatatatt gcgctggagc tggatcttaa atcggttcgc ttgcagcaat
    36421 ataaagagat ctttatttcc agcgtgatga tgctgttttg tatcggtatt gcgcttattt
    36481 ttggctggcg cttaatgcgc gatgtaaccg gtccgattcg caacatggtg aataccgtcg
    36541 accgcatccg tcgcgggcaa ctcgacagcc gggtggaagg atttatgctc ggcgagctgg
    36601 atatgctgaa aaacggtatc aactcgatgg caatgtcgct ggcggcttat cacgaagaga
    36661 tgcaacacaa tatcgaccag gcgacgtccg atctgcgtga gacgctggag caaatggaga
    36721 ttcagaacgt tgagttggat ctggcgaaaa agcgcgccca ggaagcggcg cgtattaaat
    36781 ccgagtttct ggcaaatatg tcacacgagc tgcgtacacc actgaatggt gttattggct
    36841 ttacccgcct gacgctgaaa acagaattaa caccaacgca gcgcgatcac ctgaatacga
    36901 ttgaacgttc ggcaaataat ttgctggcaa ttattaatga tgttctcgac ttctcgaaac
    36961 tggaagccgg taagctgatt ctggaaagta ttccattccc actacgcagc acgctggatg
    37021 aagtcgttac cctgctggca cattcttctc acgataaagg gctggaactg acgctcaata
    37081 ttaaaagcga cgtgcctgat aacgtgatcg gcgacccact acgattacag caaatcatca
    37141 ctaacctggt agggaatgca attaaattca ccgagaatgg caacatcgat attctggtag
    37201 aaaaacgtgc gctgagtaat accaaagtgc agattgaagt gcagattcgg gataccggca
    37261 ttggtattcc tgaacgcgat caatcgcgct tattccaggc cttcagacag gctgatgcca
    37321 gtatttcccg ccgtcatggt ggcaccgggc tggggctggt gattacacaa aaactggtta
    37381 atgaaatggg cggcgatatt tcgttccata gcctgccgaa tcgtggttca actttctggt
    37441 tccacattaa tctcgatctg aacccgaaca ttattatcga agggccatcc acccagtgcc
    37501 tcgctggtaa gcgcctggcc tatgtcgaac caaactccgc agcagcacaa tgcacgctgg
    37561 atattttaag tgaaacgccg ctggaagtgg tttatagccc aacgttctcc gcgctgcctc
    37621 ccgcgcatta cgacatgatg ttattaggca tagcggtgac cttccgcgag ccgctaacaa
    37681 tgcaacatga gcgattagcg aaagcagtat cgatgaccga tttcctgatg ctggcacttc
    37741 cttgccatgc acaagtcaat gctgaaaaac ttaagcaaga tggtatcggc gcgtgtctgc
    37801 tgaaaccatt aacacctacg cgcctgttgc ctgccctgac ggaattttgt catcacaaac
    37861 aaaacacgct tttgcctgta accgatgaaa gtaagctggc aatgacagtc atggcggttg
    37921 atgacaaccc cgctaacctg aaacttatcg gcgcattgct ggaagatatg gtgcaacatg
    37981 tggaactttg cgatagcggg catcaggcgg ttgaacgggc gaaacagatg ccgttcgatt
    38041 taatcttaat ggatattcaa atgccggaca tggacggcat tcgggcatgt gagctcatcc
    38101 accagctccc gcatcagcag caaacgccgg ttatcgcggt aacggcgcat gcgatggccg
    38161 ggcaaaaaga gaaactgctt ggcgcgggga tgagtgatta tctggccaaa ccgatcgaag
    38221 aagagcgatt gcataatttg ctgttgcgct acaagcctgg cagcggtatt tcctctcgcg
    38281 tcgtgacgcc cgaagtcaac gaaattgtgg tcaacccgaa tgcgaccctc gactggcaac
    38341 tggcactacg ccaggcagca ggaaaaaccg atttagcgcg cgatatgctg caaatgttac
    38401 tcgatttcct gcctgaagtt cgcaacaaag ttgaggaaca gctggttgga gaaaacccgg
    38461 aaggcctggt tgatttgatt cataaactgc atggcagttg cggctatagc ggtgtaccgc
    38521 gtatgaagaa tctctgccaa ctgatcgaac aacagctacg tagcggtact aaagaagaag
    38581 atttagagcc ggagctgctg gaactgttgg acgagatgga taatgtagcg cgcgaagcca
    38641 gcaaaattct cgggtaatgg acgtcttgtc gagtagcaac cggattttta tgacgctggt
    38701 ttcagattgg tgacaagtgc ctgtgtttat gccggatgcg gcgttaacgc cttatccggc
    38761 ctgcaaaacc aattaaattc aatgaattaa aaaatgatgt agccctgata cgtagcgcaa
    38821 caggcaattt tgcgtttggc atcagtctca aaccggctcc agatagagcc ggttttggtt
    38881 ttctgtctta acgcaccatg cacggacgct tgttatcgaa cgtccagccg gggatcaggt
    38941 actgcattcc catcgcatcg tcacgcgcgc caagcccgtg tttctgatac agctcgtgcg
    39001 ctttcatcac ttgatccata tcgatttcta cacccagccc cggtttttct ggcacctgaa
    39061 ccagaccgcc tttgatctct aacggttctt tggtcaggcg ctgattacct tcctgccaga
    39121 tccagtgcgt atcaatcgcg gtgattttac ccggtgcggc agcggcaaca tgggtaaaca
    39181 tcgccaggga aatatcgaag tggttgttgg agtgtgaacc ccaggtcagg ccaaattcat
    39241 gacacatttg cgccacacgt accgaacctt gcatcgtcca gaaatgcgga tccgcaagcg
    39301 ggatatcaac ggattgcaac gagagcgtat ggcccatctg ccgccagtcg gtggcgatca
    39361 tattggttgc ggtcggcagg ccagttgcgc gacggaactc tgccatcact tcacgcccgg
    39421 agaaaccttg ctccgcacca cacggatctt ctgcataagc cagcgaacct ttcaggtatg
    39481 taccgatttt aatcgcttcg ttcagcgacc aggcaccgtt aggatcgagc gtaatacgcg
    39541 cctgcgggaa acgttgcgcc agtgccacaa tagattcggc ttcttcttcc ccggccagta
    39601 caccgccttt cagtttgaaa tcgttgaagc catatttttc atacgccgct tccgccaggc
    39661 gcaccaccgc atccggcgtc atcgcttctt catgacgcag gcgataccag tcgcatgagt
    39721 catccggctg gctttgatac ggcagcggtg tagctttgcg attaccgacg aagaacagat
    39781 aaccgagcat ttcgacttcg ctacgctgtt gaccatcgcc cagcagcgat gccacgttta
    39841 cccccagatg ctgccccagc agatccagca ttgccgcttc tatcccggta actacatgaa
    39901 tagtggtacg caaatcaaat gtctgcaaac cgcgcccacc ggcatcacga tcggcaaaag
    39961 tattacgcac cagcgtcaga acgtttttgt attcacccag cgttttaccc accaccagcg
    40021 gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc gggaatttcc cctacgccag
    40081 tgtgaccgga attatctttg ataatcacaa tattacgcgt gaagaacggt gcatgtgcac
    40141 cactcagatt catcagcata ctgtcatgac ccgccaccgg gatgacctgc atttcagtaa
    40201 caacaggcgt cgtaaattga gaactcatat ttatgtcctt tttcagaatc aatgacggcc
    40261 gaaaacggga cgtttacggt caaaggtcca gccggggatc aggtactgca tcgggcctgc
    40321 gtcgttacgc gcaccgccag gcagacgttt ataggcctca tgtgcctttt gtacctgttc
    40381 ccagtccagt tccacgccca gaccgggcgc atcaggaacg gcaatttttc cgtttttaat
    40441 ctccagcgga tttttggtca gacgacaatc gccctcctgc caaatccagt gggtatcgat
    40501 agcggtagga ttacccggtg ccgccgcgcc cacatgggta aacatcgcca gagagatatc
    40561 aaagtggtta ttagaatggc agccccaggt cagcccccag tcgtcgcaaa gttgcgccac
    40621 gcggactgca ccggaaagcg tccagaagtg cggatcggca agtggaatat ctaccgcatt
    40681 gagcatcacc gcatgaccca tttcgcgcca gttggtggcg atcatgttgg tcgcgacggg
    40741 caagccggtc gcccgtcgaa actccgccat cacttcacga ccggagaaac cctgctcagc
    40801 gccgcacggg tcttccgcat aggtaagaac atcattcagc cctttgcaca gagaaatggc
    40861 ttcatcaagc agccaggcac cattgggatc aacggtaatc cgcgcatcag ggaagcgttt
    40921 cttcaatgca cgaacagtgt cgatttcttg ctcgccaggt aacacgccac ccttaagttt
    40981 gaaatcttta aagccatagc gatcctgtga ggcttccgcc agacgcacaa cggcttcgct
    41041 gttcatcgct ttctgatgcc gcaactgata ccactcatgg ttgcccggcg tattttccag
    41101 ataaggaaga tcggttttgg tccgatcacc gatataaaac agataaccga gaacggtaat
    41161 agcatcacgt tgcttgcctg gccctaacag ttcgcagacc ggaacattca gcgcctgacc
    41221 taacaggtca agcaaagcgg cttccagcgc cgccacggca ttaacgcgca attcaaaagt
    41281 ccaggcacct ttgccgaagg tatcaaaatc ggctgcctga ttacctttat gcacctgctg
    41341 aaccacttta ttcaggcgcg caacttcctg gcccagcacc atcggaatag cctcgaccag
    41401 tgtttgataa atcacctctc cgcccggcgc ttcaccaatg ccggtatgcc cggcgttatc
    41461 ggtgagtacc acaatattgc gggtgaaata tgcgttatgt gcaccaccaa tattaagcaa
    41521 catgctgtca tgtccggcga ccggaatgac tttcatatca gtaataacag gactggattg
    41581 tgtcgtcatc ttattgcccc acgacaggtt tcagctcaat acgcttgata tcgcccacca
    41641 gcaccaggta gctgagtacc gcgattaagg catgaacacc aacataaatc agcgccccat
    41701 taaaggagcc agtcgtgcca acgatataac caattgcgat tggcgtgacg atgccagaaa
    41761 tgttaccaaa catgttgaac aggccaccgg aaagaccgct gatctctttt ggcgcagtat
    41821 cagccattac agcccaaccc agcgccccga tccccttacc gaagaaggcc agcgccataa
    41881 agccgatgat catccactca acgttgacgt agttgcagaa caccatcacc atcgacaaca
    41941 acatgcccat cacgatcggt gttttacgcg caatgttcag tgaacctgtg cgacgcatca
    42001 gccagtcgga aataatcccc cccaacacac cgccgataaa accgcaaacc gccggaacag
    42061 aagccacaaa gcctgctttc agaatcgaca tcccgcgcgc ctgcaccaga taaaccggga
    42121 accaggtaat aaaaaagtaa gtcagggcgt tgatacagta ctgaccgata taaacgccga
    42181 tcatcatccg tgacccgagc agctgtttga tctgccccca cttcacgctg aacggcactt
    42241 taactttggt gttttgctga tccatattga tcagcgcgcc acccgcggcg atgtactcca
    42301 gctctttctg atttacgccc ggatgttgat ttggctcgtg gatgactttc aaccagataa
    42361 agctgatgac aatgcccaga ccgcccataa agaagaagac gtgtgaccag cccacttcat
    42421 gagtcaacca gcccataatc ggcgcgaaga taaccgttgc gaagtattgg gcggagttaa
    42481 aaatcgacac cgccgttccc ctttcctgcg ccggaaacca ggccgcaaca atgcggctgt
    42541 tgccagggaa agagggcgct tcagcaagcc cgaccaggaa gcgcagtgta aacagggcaa
    42601 cgataatgcc gaatccacta aagatatcga cgaagccttg cagcaaggta aacatcgacc
    42661 agataaagat cgaccagaag tagacgcgtt ttgaaccaaa acgatccagc aaccagccac
    42721 cagggatctg cccgataaca tacgcccatg agaaggcaga gaacacatag cccattccca
    42781 cgggatcaag gccgatatct ttggccattt ccgaaccggc aatagaaagc gtagcgcggt
    42841 cgccgtagtt gaaggatgtg acgataaaca acatcaccac tatccagtaa cgagcattcg
    42901 tgcgtttttc cacactgctc gcagcctgac ttaaagaact cattgttgca ctcctgaaaa
    42961 ttcgcgttag ccacgctcac tctggactgc gacatcgcca ggaaatcaga ggtgacgtag
    43021 ggtgtttttg ccgtttttat aggtcgttcg ccgaatacgg cgcgtgttta tatctggcaa
    43081 tagcagtata aaaagcgcgc catagcggct caccgtgcaa caacacaaca ttaatgcgtt
    43141 caatgaggcc cgattttggc attagccctg gacggtggaa tccacttcac ggaaatgaaa
    43201 acaagaacaa gaaaggaagg gttaaaacga agaaataaag aagagtatga aatggatcgc
    43261 ttgactccag gcaaacgcca gtaaaaatcc gctctatgaa gcagttttta ctggcatttg
    43321 cctgaaaaga ttcgattcag caccgctaaa acggcattta ccgctcgctg aacatatcag
    43381 gacaacatcg tgccccaatg ttcgacccac ggatttgatt ccgtttccgg ttccgggttc
    43441 tcactggcgt caatcaacag catttcgcca acccgctgag cgctctgttc ctgcaacaag
    43501 gcatcgaact gtttgccgcc attgcagaaa ttcacataac tactgtcgcc gagcgcaatc
    43561 acgccataac gcagattcgg ctggaagccc agactatctt tgattccctg aaagagtggc
    43621 acaatgctat caggaaggtc gccctgcccg gtcgtggacg taaccaccag aacatactta
    43681 tcctgatagg gcagccagtc gcttaattca ggatcttcaa ataccgttgc tttgtggccc
    43741 tgcgcggtca gaatcgcttc cgcttcttcg gccactaaca gtgaattccc gtacatggtg
    43801 ccgacaaaaa taccaatttc cgccataccg ttttctccct ggattaggaa cttatctctc
    43861 catcctgacc cgatggcgca ctgaactcaa ccctttcatt ttcaggaagc agaccccgcc
    43921 agccaaattg tgataacgcc tgcatccagg tgtcgtccag gcccgcgtga atagtcagcg
    43981 gctcgccagt aaaaggatgc gtcaatgaca actgactagc atgcagcatt aaccgctgaa
    44041 ggccaaaatg ttcagcaccg ctgcgattct ggcgtaaatc accatgtttg ctgtcgccaa
    44101 taatcgggtg gcgcaaatgg gcaagatggc gacggagctg gtgcttacgt ccggttttcg
    44161 gctccagttc taccaggccg tagcgcgtgg tcgggtaacg tccggtcgct accggcattt
    44221 ctacggtcgc cagaccgcga taatgcgtca ctgctggctg cgggccttta tcttcgcggg
    44281 caaatttatc agcgattttg tccagttctt ccaccagtgg ataatccagc accgcttctt
    44341 ccatcaacca gccgcgcaca atcgcatggt aacgtttctg gatttggtgc tgttcaaact
    44401 gttgtgccag cagccgtccg gcctcgctgg ataatcccat caacaacaca ccagaagtgg
    44461 gtcggtccag acgatgagca gtaaacacat gctggcctat caggtcacgc acggtttgca
    44521 tgaccactac tttctcgtcg cgatccagcc agctgcggtg aaccagccag ccggagggtt
    44581 tatttaccgc aaccagccat tcatcctgat agagtatttc cagcattagc tcgcatcatc
    44641 cgcaaaaaga gcatccagtt tttccagctc agccagaata agcgcgcgtt gcgggtggtc
    44701 cgtcgccagc gccatttcat aatagggcgc aacagcaaaa gcgcccggta acggctggtt
    44761 gttatctaac aaatcgtgca tccgcggaat cagtacccat tgcaaccact ccagtggttc
    44821 catggtgtcc ataaagaacg gctgggtact gttaaattga tgcggcaggg gttcatcgtt
    44881 tcgccagtgc tgatgttcac gcagtaacgc ttcaagcgcc tgcaactgga gacgaacgcg
    44941 gtcatgagtg gtcataaagc aacctcaata agaaaaactg ccgcgaagaa tagcaggaat
    45001 aaaaaaaggg agcactgtat tcacagtgct cccggttcgt ttcgcagcat tccggctact
    45061 tttgttgctc cctgctcatc cttgacaact tttcctctgg ccttgcggcc aatcgttcat
    45121 cctgaactat tgcttcctgc tcacaccacc ccgatgtgat acttcatcct gaagtgttcc
    45181 tggccttcct gacccaccga atcatcctga ccggttctca ttctccatcc tggaggtgtc
    45241 ctttaacgcg tcctgcgtca tcctcttcgc ttcatccaga agcctttccc tgaaacacca
    45301 tcctggtgtg tccttcagaa gtgtcatcat cctgatgttc acttcgttgt ctgactccct
    45361 gtcgacgaag atagaatcgt ctttttctcg aagtcttaca aggcgcttac agacaatgcc
    45421 ttaaggaaaa tttcatatga aagttaagaa ttaacaagca acatgttgtt tttaaatgag
    45481 aataacgatt cttatctttt taagtattcg cttatttcct ggcgatctcc tacagagtct
    45541 gtaagagatc tctcacaacc agatggggta tttggattac agaagaggct taagttgatt
    45601 aaggaattcc gcaagatttg aggctaaatg cgtccgttta cgtgttccca gcgtctcttt
    45661 gcacacttca ccggagagat tacacaccga gatgacctct aactcttctt ccagcgtcgc
    45721 gataaatagc gtgggcggca gttttagcct tttctgagtc accagatgac caatcagatt
    45781 ttcctgcacc cgacggaagt cgtcttcact ccaggtttgc agcaatgtca gtttgatatc
    45841 gccaaactgc gcgtgcatat ccccggcaaa ctgagtcgta taaaaggtat gaattgtggg
    45901 ttgtatcaca atatcaaaag cgcgttcaac cgcgtttaca ttttgttccc cggtaaacgg
    45961 ctggggttgc cagtatacgg catcttcggt agttgaaata atgcacggtg aaggaacgcc
    46021 gtacagttcc tcacttaacg gccagctttt atgctcttca tgccacgcat cgcagtagcg
    46081 tgcagtaaaa tctttcaggg cttgtgcggt caaatcgtcc actaatttct ctcttcactt
    46141 aaaccagata cacttgtcgt ttagtttatc tggtttatga cggtgaaaca tgtcttctta
    46201 tgcaaaccat caggcacttg cgggcctgac acttggaaaa tcaaccgatt accgggatac
    46261 ctatgacgcc agcctactgc aaggcgtacc acgcagcctg aatcgcgacc cgctgggtct
    46321 gaaagcggat aacctgcctt ttcacggtac ggatatctgg acgctgtatg aactttcctg
    46381 gctgaatgcg aaaggtttgc cgcaggtcgc tgtcggtcat gttgaacttg attacaccag
    46441 cgtaaatctg attgagtcga agagttttaa gctctatctc aacagtttta accagacgcg
    46501 ttttaataac tgggatgagg tgtgccagac gctggagcgc gacttaagca cttgcgccca
    46561 gggtaaggtt agcgtggcgt tatatcgtct tgatgaactg gaaggccagc cgataggtca
    46621 tttcaatggc acctgcattg atgaccagga tatcactatc gataactatg aattcactac
    46681 tgactatctg gagaatgcca ccagcggtga aaaagtagtg gaagagacgc ttgtcagcca
    46741 cctgctgaaa tcaaactgcc tgatcaccca tcaaccagat tggggttcca tccaaattca
    46801 gtatcgtgga cgccaaattg acagagaaaa actgctgcgt tacctggtct ctttccgtca
    46861 tcacaacgag ttccacgaac agtgcgtgga acgcatcttt aatgacctgt tacgcttctg
    46921 ccagccagag aaattgagcg tttacgcacg ttatacccgt cgtggcggtc tggacattaa
    46981 cccgtggcgc agtaatagcg attttgtccc atcgaccaca agactggttc ggcaataaat
    47041 tttttctcaa ttttgcgtgc tggattcacg cagaaggttg tgaaaggtca gcaggcaggg
    47101 ctattgtaat caaagggaat gacgatattc gtcccataag gagtttttct tgattacaca
    47161 tattagcccg cttggctcca tggatatgtt gtcgcaactg gaagtggata tgcttaaacg
    47221 caccgccagc agcgacctct atcaactgtt tcgcaactgt tcacttgccg tactgaactc
    47281 cggtagtttg accgataaca gcaaagaatt gctgtctcgt tttgaaaatt tcgatattaa
    47341 cgtcctgcgc cgtgaacgcg gcgtaaagct ggaactgata aatcccccgg aagaggcttt
    47401 tgtcgatggg cgaattattc gcgctttgca ggccaacttg ttcgcggttc tgcgtgacat
    47461 tctcttcgtt tatgggcaaa ttcataacac cgttcgtttt cccaacctga atctcgacaa
    47521 ctccgtccac atcactaacc tggtcttttc catcttgcgt aacgctcgcg cgctgcatgt
    47581 cggtgaagcg ccaaatatgg tggtctgctg gggcggtcac tcaattaacg aaaacgagta
    47641 tttgtatgcc cgtcgcgtcg ggaaccagct gggcctgcgt gagctgaata tctgcaccgg
    47701 ctgtggtccg ggagcgatgg aagcgccgat gaaaggtgct gcggtcggac acgcgcagca
    47761 gcgttacaaa gacagtcgct ttattggtat gacagagccg tcgattatcg ccgctgaacc
    47821 gcctaacccg ctggtcaacg aattgatcat catgccagat atcgaaaaac gtctggaagc
    47881 gtttgtccgt atcgctcacg gtatcattat cttccctggc ggtgtgggta cagcagaaga
    47941 gttgctgtat ttgctgggaa ttttaatgaa cccggccaac aaagatcagg ttttaccatt
    48001 gatcctcacc ggcccgaaag agagcgccga ctacttccgc gtactggacg agtttgtcgt
    48061 gcatacgctg ggtgaaaacg cgcgccgcca ttaccgcatc atcattgatg acgccgctga
    48121 agtcgctcgt cagatgaaaa aatcgatgcc gctggtgaaa gaaaatcgcc gcgacactgg
    48181 tgacgcttac agctttaact ggtcaatgcg cattgcgcca gatttgcaaa tgccgtttga
    48241 gccgtctcac gagaatatgg ctaatctgaa gctttacccg gatcaacccg ttgaagtgct
    48301 ggctgccgac ctgcgccgtg cgttctccgg tattgtggcg ggtaatgtaa aagaagtcgg
    48361 tattcgcgcc attgaagagt ttggtcctta caaaatcaac ggcgataaag agattatgcg
    48421 tcgtatggac gacctgctac agggttttgt tgcccagcat cgtatgaagt tgccaggctc
    48481 agcctacatc ccttgctacg aaatctgcac gtaatctccc ctcccccggt gacgtttgtt
    48541 gccgggtttt ctcgtttttg gtcgcttact catcaactca tttcatttgt tatatgaatg
    48601 tttcttacca ccctcacgcg acaaatatca ccacagttaa tatgtcatac aatttatgtt
    48661 aaaacgcaaa cgtttcccta ttttcataaa ccacatttta tcgccatgaa aaagaaatta
    48721 tcgccatgat taactaaaag tattgatttt ttcagttcaa cctacatata ttgcgcaccc
    48781 cgtaagaagt cagatgtcgt ttaatgggta aatattgccc ttaaattctc ttttactttt
    48841 gatttacaga gtaaagcgtt gtgattatct atcttccaag tagattattg tatttgggat
    48901 caagatcact gatagataca taacttgtgt gtatctttcc gccctcaaat tattacggtg
    48961 gaaaatgatt aagccatcgc cgatagacag atttcatttt tacggtcagg caccttcccg
    49021 ggctgaactg gctaaaagct gaattatttg cattcctcca ggagaaatag atggaaacga
    49081 ctcaaaccag cacgattgcg tcgaaagact ctcgtagtgc ctggcgcaag acagacacca
    49141 tgtggatgct gggcctttac ggcacggcaa tcggcgcggg cgtgctgttc ctgccaatca
    49201 acgctggtgt tggcggtatg atcccgctga tcatcatggc tatccttgcg ttcccgatga
    49261 cgttttttgc tcaccgcggc ctgactcgct tcgtactgtc tggtaaaaac ccgggcgaag
    49321 acatcaccga ggttgtagaa gaacactttg gtattggcgc aggtaaactg attactctgc
    49381 tctacttctt cgctatctac ccgatcctgc tggtttatag cgtggcaatc accaatacag
    49441 ttgaaagctt catgtctcac cagctgggta tgacgccacc gccgcgtgcg attctgtcgc
    49501 tgatcctgat cgtgggtatg atgaccatcg ttcgcttcgg tgagcagatg atcgttaaag
    49561 cgatgagtat tctggtattc ccgtttgttg gcgtactgat gctgctggct ctgtacctga
    49621 tcccgcagtg gaacggcgca gcactggaaa cgctgtctct ggacactgca tctgcaaccg
    49681 gaaacggtct gtggatgaca ctgtggctgg caattccggt aatggtgttc tcgttcaacc
    49741 actctccgat catctcttct ttcgccgttg cgaagcgtga agagtacggc gatatggcag
    49801 aacagaaatg ctcgaagatc ctggcattcg cacacatcat gatggtgctg accgtaatgt
    49861 tcttcgtctt cagctgcgta ctgagcctga ctccggcaga cctggctgcg gctaaagagc
    49921 agaacatctc gattctgtct tacctggcta accactttaa cgcgccgatc atcgcgtgga
    49981 tggctccgat tatcgcgatt atcgctatca ccaaatcctt cctcggccac tacctgggcg
    50041 cacgtgaagg cttcaacggt atggtgatta aatctctgcg tggtaaaggt aagtctatcg
    50101 aaatcaacaa gctgaaccgt atcactgcgc tgttcatgct ggtaacgacc tggattgttg
    50161 ccaccctgaa cccgagcatc ctgggtatga ttgaaaccct gggcggccca atcatcgcga
    50221 tgatcctgtt cctgatgccg atgtacgcaa ttcagaaagt accggcaatg cgtaaataca
    50281 gcggtcacat cagcaacgta ttcgttgtcg tgatgggtct gattgcaatc tccgcaatct
    50341 tctactctct gttcagctaa gtcctttcgc gccgctttcg ggcggcgctt cctccgtttt
    50401 aacgcgatgt atttcctatg attagcgtat tcgatatttt caaaatcggc attggccctt
    50461 ccagttctca taccgttgga ccaatgaaag cgggtaaaca atttaccgac gatctgattg
    50521 cccgtaacct gcttaaagac gtgacccgcg tggtggttga cgtgtacggc tcgctctctc
    50581 tgaccggtaa aggccaccac acagatatcg ccataattat gggtctggcg ggtaacctgc
    50641 cggataccgt ggatatcgat tccatccctg gttttattca ggatgtgaat actcatggtc
    50701 gcctgatgct ggcaaacggt cagcatgaag tggagttccc ggttgatcag tgcatgaact
    50761 tccatgccga caacctttct ctgcatgaaa acggtatgcg cattaccgcg ctggcgggcg
    50821 ataaagtcgt ttacagccag acttattact ctatcggcgg tggctttatt gttgatgaag
    50881 aacattttgg tcagcaggat agcgcaccgg ttgaagttcc ttatccgtat agttccgctg
    50941 ccgatctaca aaaacattgt caggaaaccg ggctgtcact ctctggcctg atgatgaaaa
    51001 acgagctggc gctgcacagc aaagaagagc tggaacagca cctggcgaac gtctgggaag
    51061 tcatgcgtgg cggtattgag cgcggtattt ccaccgaagg cgtgttgcct ggcaaactgc
    51121 gcgttccacg ccgtgctgcg gcactacgcc ggatgctggt cagccaggat aaaaccacca
    51181 ctgacccgat ggcggttgtt gactggatca acatgtttgc actggcagtg aacgaagaga
    51241 atgctgctgg cggtcgcgtg gtgactgcgc cgactaacgg tgcgtgcggg attattccgg
    51301 ccgtgctggc gtactacgac aagtttatcc gcgaagtgaa cgctaactca ctggctcgtt
    51361 acctgctggt agccagcgcc attggttctc tttataagat gaacgcctcg atttctggtg
    51421 ccgaagtcgg ctgccagggt gaagtcggcg tggcgtgctc aatggcggca gctggtctgg
    51481 cagaactgtt aggcgcaagc ccggcgcagg tatgcatcgc ggcggaaatc gccatggagc
    51541 acaacctcgg tctgacgtgt gacccggtcg ccgggcaggt acaggtgccg tgcatcgagc
    51601 gtaacgccat tgcggcagtg aaagcagtaa acgctgcacg tatggcgctg cgccgtacca
    51661 gcgagccacg cgtctgcctc gacaaagtta tcgaaaccat gtacgaaaca ggtaaagata
    51721 tgaacgccaa gtaccgtgaa acgtctcgcg gcggcctggc aatgaaaatc gttgcctgcg
    51781 attaatcgct ctccagaggc ctctttttgc gaggcctctt cccgatttct catccagcca
    51841 tagcctgttc tggcatcgaa tgttaccctt atcgcctgat ctttaagggg gttatcgtgg
    51901 ctgttcattt gcttattgtc gatgcactga atcttattcg tcgcattcat gccgttcagg
    51961 gttcgccctg tgtcgaaacc tgccagcacg cgctcgatca gctcattatg cacagccaac
    52021 caacccacgc ggtcgccgtt tttgatgatg aaaaccgcag tagcggctgg cgtcatcagc
    52081 gtttaccaga ttacaaagcg ggtcgaccgc caatgccgga agagttgcac aacgagatgc
    52141 ctgcattacg cgccgccttt gagcaacgcg gcgtcccgtg ctggtccgcc agcggcaacg
    52201 aagccgatga cttagccgcc acgctggcgg tcaaagtgac acaggccggg catcaggcaa
    52261 cgattgtttc gacagataaa ggctacagtc agttactttc accgacatta cgtattcgtg
    52321 attacttcca gaaacgttgg ctggatgcgc catttatcga taaagaattt ggcgttcaac
    52381 cgcagcagtt gcccgattac tgggggcttg cggggatcag cagttcaaag gtaccgggtg
    52441 ttgcggggat cggaccaaaa agcgccacac agctactggt cgagtttcag agtctggaag
    52501 ggatatatga gaatctggat gcagttgccg aaaagtggcg caaaaaatta gaaacccata
    52561 aagagatggc gtttctgtgc cgcgatattg cccgcttaca aaccgatttg catatcgacg
    52621 gcaatttaca gcaattgcgg ttggtaaggt aacggcgtgc cggatgcaaa cgattgtcaa
    52681 cacattgaat ttaatgcact tatatgccgg ataagacgcg gctagcgtcg catccggcat
    52741 tatcacatcc gcgcaattac caggcggtat ggtaaagctc tacaatatcc tcaagcgttg
    52801 cttcacgcgg gttgccaccg gtacaaacat catccagtgc cgcctgcgcc agtgccggaa
    52861 tgtcttcctt gcgtacacca acatcacgta aatgtggcgg aataccgaca tcacggttga
    52921 gagcaaacac cgcttcaaca gcggcattac gcgcctcttc caggctcata ccttccactt
    52981 tcacgcccat aacgcgcgcg atatcgcggt acttctcacc ggtaaagtca gcgttatagc
    53041 gcatgacatg cggtaacagg atggcatttg caacaccgtg tggcgtgttg taaaacgcgc
    53101 ccagtggatg cgccatacca tgcaccaacc ctaacccaac attcgagaat cccatacccg
    53161 caacatactg cccgagcgcc atttcttctc cggcatcctt atcaccagca accgatcctc
    53221 gcagcgcccc agcaatgatt tcaatcgctt taatgtgcag cgcatcggtt agcgcccacg
    53281 cgccacgggt aatatacccc tcaatagcat gagtgagcgc atcgacaccc gtcgcggctt
    53341 tcagcgctgg aggcatacca tccatcatgt cagcgtcaat aaacgccacc tgcgggatat
    53401 catgcggatc aacgcaaaca aacttgcgcc gtttctcttc gtcagtgatc acgtagttaa
    53461 tggtcacttc tgccgcagtg cctgctgtgg tggggattgc cagaatcggt acactgggtt
    53521 tattggtcgg ggaaagccct tccaggctac gcacatcggc aaactccggg ttgttgctga
    53581 taatgccaat cgctttacac gtatcctgtg gagaaccacc accaatagcg atcaggtaat
    53641 ccgcgccgct attctggaat acatcgagcc cttctttgac gacagtaatt gttgggttag
    53701 gtactacgcc gtcgtaaatc gcccatgcca gtcctgccgc atccatctta tcgatcactt
    53761 tcgccaccac gccgcattgc accagcgttt tatcggtgac gatcagcgcc ttctgataac
    53821 cacggcgttt cacctcatcg gttaaagacc caacagcccc ccgaccaaac catgccgttt
    53881 cgttcagaat cattctgtta gccatcatcc ttctccttgt tgctttacga aattactctt
    53941 caatccgtaa cccgtaggtt ttgaatttct ccagcactac ggcaatctct tcatcgctca
    54001 gcactggcac cgggtccgta atcgccaggg tcgtcaggta aagttgtgcc agcacttcaa
    54061 cttcatgcgc cagccataac gctttctcca gattcgcctc acaagcgata agcccatgat
    54121 gttgtaacaa agttgcctta cgatttttga gagccagcgc aacatgttca gaaagttcgc
    54181 gtgttccaaa ggtcgcataa ggcgcgcaag gaatagaatt accgccagcc gccgcaatca
    54241 tgtagtgaat cgcggggatc ggtcggttaa gaatggaaac tgccgtacaa tgaacggcat
    54301 gattgtgaac aaccgcgttg gcatccggtc tgctttgata ggccgccata tggaaacgcc
    54361 actcgcttga ggggagtttt ccttcctcat gtttgccgtt gccatcaata aagacaatat
    54421 gcgactccgt cagtttttca tatggaatac ctgtaggcgt aatcagcatc ccatcctgat
    54481 aacgtacact gacgttaccc gctgtcccct ggttcagccc caggcgagtc atttccaggc
    54541 aagtgtcaat aatctgacga gcaagtttat ttcgttccat tagctacctc tctctgattc
    54601 aaaacagggc aataatgttg ttcctttcac actattgaat tagccgttta attacccacc
    54661 atcttcttcc tgattaacca gaaagaaatt cacaagctta tattttgtga cctggttcaa
    54721 ctaatcacag taaataactg caagttctct ttttataact ccattaaaaa tgaccgctct
    54781 taaaaatatt tatcaaaacg gtcatttttc tactcctccc tgccaggaat gcccgttttc
    54841 ggcacaaaca attaatacgg tcatctggtt tgtgtttttt atgatttatt ttctgaaacg
    54901 ggcatgaaat ttcgattatt aaagtgatgg tagtcacata aagtcacctt ccagctaata
    54961 agtgtgaccg ccgtcatatt acagaccgtt ttttatttga aaatgaatcc atgagttcat
    55021 ttcagacagg caaatattca ctgatatgaa gcccgaactc gctggttttg cacttttgaa
    55081 aacataaccg attacgtgct taagcttctg aacctaagag gatgctatgg gaaacacatc
    55141 aatacaaacg cagagttacc gtgcggtaga taaagatgca gggcagagca gaagttacat
    55201 tattccattc gcactgctgt gctcactgtt ttttctttgg gcggtagcca ataaccttaa
    55261 cgacatttta ttgcctcaat tccagcaggc ttttacgctg acaaatttcc aggctggcct
    55321 gatccaatcg gccttttact ttggttattt cattatccca atccctgccg ggatattgat
    55381 gaaaaaactc agttataaag cagggattat taccgggttg tttttatatg ccttcggcgc
    55441 tgcattattc tggcccgccg cagaaataat gaactacacc ttgtttttag ttggcctatt
    55501 tattattgca gccggattag gttgtctgga aactgccgca aacccttttg ttacggtatt
    55561 agggccggaa agcagtggtc acttccgctt aaatcttgcg caaacattta actcatttgg
    55621 cgcaattatc gcggttgtct ttgggcaaag tcttattttg tctaacgtgc cacatcaatc
    55681 gcaagacgtt ctcgataaaa tgtctccaga gcaattgagt gcgtataaac acagcctggt
    55741 attatcggta cagacacctt atatgatcat cgtggctatc gtgttactgg tcgccctgct
    55801 gatcatgctg acgaaattcc cggcattgca gagtgataat cacagtgacg ccaaacaagg
    55861 atcgttctcc gcatcgcttt ctcgcctggc gcgtattcgc cactggcgct gggcagtatt
    55921 agcgcaattc tgctacgttg gcgcacaaac ggcctgctgg agctatttaa ttcgctacgc
    55981 tgtagaagaa attccaggta tgacagcagg ctttgccgct aactatttaa ccggaaccat
    56041 ggtgtgcttc tttattggtc gtttcaccgg tacctggctc atcagtcgct tcgcaccaca
    56101 caaagtcctg gcagcctacg cattaatcgc tatggcactg tgcctgatct cagccttcgc
    56161 tggtggtcat gtgggtttaa tagccctgac tttatgcagt gcctttatgt cgattcagta
    56221 cccaacaatc ttctcgctgg gcattaagaa tctcggccag gacaccaaat acggttcgtc
    56281 cttcatcgtt atgaccatta ttggcggcgg tattgtcact ccggtcatgg ggtttgtcag
    56341 tgacgcagcg ggcaacatcc ccactgccga actgatcccg gcactctgct tcgcagtcat
    56401 ctttatcttt gcccgtttcc gttctcaaac ggcaactaac tgaacatatt ttccgaataa
    56461 agtgaggaat cagtaatgaa aaaaatcagc ttaccgaaaa ttggtatccg cccggttatt
    56521 gacggtcgtc gcatgggtgt tcgtgagtcg cttgaagaac aaacaatgaa tatggcgaaa
    56581 gccacggctg cactgctgac cgagaaactg cgccatgcct gtggagctgc cgtcgagtgt
    56641 gtcatttccg atacctgtat cgcgggtatg gctgaagccg ctgcttgcga agaaaaattc
    56701 agcagtcaga atgtaggcct caccattacg gtaacccctt gctggtgcta tggcagtgaa
    56761 accatcgata tggatccaac ccgcccgaaa gccatttggg gctttaacgg cactgaacgt
    56821 cccggcgctg tttacctggc agcggctctg gcagctcaca gccagaaagg catcccggca
    56881 ttctccattt acggtcatga cgttcaggat gccgatgaca catcgattcc tgccgatgtt
    56941 gaagaaaaac tgctgcgctt tgcccgcgcc ggtttagcag tcgccagcat gaaaggtaaa
    57001 agctatctgt cgctgggcgg cgtttcgatg ggtatcgccg gttccatcgt tgatcacaac
    57061 ttctttgaat cctggctggg aatgaaagtc caggcggtgg atatgaccga actgcgtcgc
    57121 cgtatcgatc agaagattta cgacgaagcc gaactggaaa tggcgctggc ctgggctgat
    57181 aaaaacttcc gctatggcga agatgaaaat aacaaacagt atcaacgcaa tgccgagcaa
    57241 agccgcgcag ttctgcgcga aagtttactg atggcgatgt gtatccgcga catgatgcaa
    57301 ggcaacagca aactggccga tattggtcgc gtggaagaat cacttggcta caacgccatc
    57361 gctgcaggct tccaggggca acgtcactgg accgatcaat atcccaatgg cgataccgct
    57421 gaagcgatcc tcaacagttc atttgactgg aatggcgtgc gcaaaccgtt tgtcgtggcg
    57481 accgaaaacg acagtcttaa cggcgtggca atgttaatgg gccaccagct caccggcacc
    57541 gctcaggtat ttgccgatgt ccgtacctac tggtcatcag aatcaattga gcgtgtaacg
    57601 gggcataaac tggacgggct ggcagaacac ggcatcatcc atttgatcaa ctccggttct
    57661 gctgcgctgg acggctcctg taaacaacgc gacagcgaag gtaaaccgac gatgaagccg
    57721 cactgggaaa tctctcagca agaggctgac gcttgcctcg ccgctaccga atggtgcccg
    57781 gcgatccacg aatacttccg tggcggcggt tactcttccc gcttccttac cgaaggcggc
    57841 gtcccgttca ccatgactcg tgtcaacatc atcaaaggcc tgggaccggt actgcaaatc
    57901 gcggaaggct ggagcgtgga attgccgaag gatgtgcatg acatcctcaa caaacgcacc
    57961 aactcaacct ggccaaccac ctggtttgca ccgcgcctca ccggtaaagg gccgtttacg
    58021 gatgtgtact cggtaatggc gaactggggc gctaaccacg gagttctgac catcggccac
    58081 gttggcgcag actttatcac tcttgcctcc atgctgcgta tcccggtatg tatgcacaac
    58141 gttgaagaga ccaaagtgta tcgtccttct gcctgggccg cgcacggcat ggatattgaa
    58201 ggccaggatt accgcgcttg ccagaactac ggcccgttgt acaagcgtta atacattact
    58261 ccctgatgtg atgcccggtc gctccggcta ccgggcctga acaagcaaga gtggttagcc
    58321 ggataagcaa tgttatccgg ctatattgca ggagcgatta tgaaacaaga agttatcctg
    58381 gtactcgact gtggcgcgac caatgtcagg gccatcgcgg ttaatcggca gggaaaaatt
    58441 gttgcccgcg cctcaacgcc taatgccagc gatatcgcga tggaaaacaa cacctggcac
    58501 cagtggtctt tagacgccat tttgcaacgc tttgctgatt gctgtcggca aatcaatagt
    58561 gaactgactg attgccacat ccgcggtatc gccgtcacca cctttggtgt ggatggcgct
    58621 ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg tccgcgaaca
    58681 gccgcggtaa tggacaatat tgaacggttg atctccgcac agcagttgca ggctatttct
    58741 ggagtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa agaaaatcat
    58801 ccacaactgc tggaacgcgc gcacgcctgg ctctttattt cgtcgctgat taaccaccgt
    58861 ttaaccggcg aattcactac tgatattacg atggccggaa ccagccagat gctggatatc
    58921 cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg ccgactcttc
    58981 cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacagcgc cgcagcaatg
    59041 ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca gttcgccctt
    59101 tttggcgctg gtgccgaaca aaatgaaccc gtgctttctt ccggtacatg ggaaatttta
    59161 atggtccgca gcgcccaggt tgatacttcg ctgttaagtc agtacgtcgg ttctacctgc
    59221 gaactggata gccaggcagg gttgtataac ccaggtatgc aatggctggc atccggcgtg
    59281 ctggaatggg tgagaaaact gttctggaca gctgaaactc cctggcaaat attgattgaa
    59341 gaagctcgcc tgatcgcgcc tggcgcagat ggagtgaaaa tgcagtgtga tttattgtcg
    59401 tgccagaacg ctggctggca aggcgtaacg cttaatacca cgcgggggca tttctatcgc
    59461 gcggcgctgg aagggttaac cacgcaatta cagcgcaatc tacagatgct ggaaaaaatc
    59521 gggcacttta aagcctctga attattgtta gtcggcggag gaagtcgcaa cacattgtgg
    59581 aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga tgccgaaacg
    59641 accgtcgcag gagctgcgct gtttggttgg tatggcgtag gggaatttaa cagcccggaa
    59701 gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac tgaacctgaa
    59761 tttatagagg aggtgtgaaa tgctgaaaac aatttcgccg ttaatttctc ccgaactatt
    59821 gaaagtgctg gcagagatgg ggcatggaga tgaaattatt ttttccgatg ctcactttcc
    59881 cgcccattcg atgggaccgc aggtgatccg cgctgatggc ctgttggtga gcgacttgct
    59941 ccaggcgatt atcccgttat ttgagctgga cagttatgca ccaccgctgg tgatgatggc
    60001 ggcggtagaa ggtgacactc tcgatcctga agtagaacga cgttatcgta atgcgctttc
    60061 actacaagcc ccctgtcctg acatcatccg catcaatcgt tttgcgtttt atgaacgggc
    60121 gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga atattctttt
    60181 aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg ggcggttatc
    60241 gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg acctgctgct
    60301 gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca gtaaagaaac
    60361 cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca atcatgggcg
    60421 cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca tccggctgaa
    60481 aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga ttgaagaagg
    60541 gatggtgata gccttagacg ccagttcaac ctgctggtat ctggcacgcc agttgcctga
    60601 catcaacatc caggtcttca ccaatagcca tccgatttgc catgaactcg gtaaacgcga
    60661 acgcattaaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct gttacgtcaa
    60721 tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt tttcttgtga
    60781 agggatcgat agcagcggcg cactgtggga ctccaatgcc atcaacgctg attacaaatc
    60841 aatgttatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta aatttaatcg
    60901 ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt ctgatgagcg
    60961 ccaggttaca acttctttgg taacagcctg acggaaaggg tagcaggccg gagacgacgc
    61021 cccggcctta cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca gatacggcgg
    61081 acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat ctgagcattt
    61141 atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgacac ttcttcgtag
    61201 cgttttttca tcggcagttt gagattgaag atagtttcac ggcaccagcc attaaccagc
    61261 cactgcgcca ttaatgccgc aactttcgcc ggtttttcaa ccatatcgca taccatccag
    61321 gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca cgtcacctgc
    61381 ccagtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac ccacatgttg
    61441 cgcttcacca gttggtaggt ccagccgcct gggcaagcac ctaaatccac cgcccacatc
    61501 ccgttcgcca ggcgttcatc ccactcatcc gcgggaataa acacatgaaa tgcctcttcc
    61561 agtttgagcg tggaacgact cggcgcatct gccggaaatt tcaggcgcgg aatgcccata
    61621 tagaacggcg aattattgct gctgtatgag taaccagtat agcaacagcc tggtgcaata
    61681 aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac ccccgcatca
    61741 cgcaaggcag cgcgtagcgg cacggtgaat ttacggcaga atttcagcaa ctctttgctt
    61801 tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac gccctgtaac
    61861 atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag ttcccccact
    61921 acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat taacttatcg
    61981 ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt cacgcgggca
    62041 aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca ctctttttca
    62101 aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc gctttaaacg
    62161 gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc caaccggtgt
    62221 gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc cgctgaacag
    62281 caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga tgcgacgctg
    62341 cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt cgaggccggt
    62401 ctggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg cgccaaaagc
    62461 gcccagagct acaaaaatga agccgctaat ggcggcgaaa atcagcataa aacggctggt
    62521 catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt cttgttcagc
    62581 agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt ctgcctggct
    62641 gtcatgacaa accagataaa aagcgttttt actgaccaga acatcattaa acgggcaaac
    62701 aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg ccactccctg
    62761 cccgtggata gccgcttgca gcaccatggc gctatggcta aaaattggcc cttgctgaac
    62821 gttgatatga tttaacccca actgtcgggt atatgtctgc cagtcacggc gtgacgcatc
    62881 atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt cgccagtcag
    62941 cagtagcggc gagcacaccg gcaataaata ttcggcgtac agtttttcca cccgtagccc
    63001 cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca gcttatcttc
    63061 ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat taaagctgga
    63121 aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg tcaacgcccc
    63181 cttagcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa atatctcttt
    63241 gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat tacggcggcg
    63301 gaacagtttt agccccagaa aatcctcaag agacttgatt tgatgactta ctgcggcctg
    63361 ggtcacaaaa agctcttctg ctgcgcgagt gaaacttaaa tggcgtgctg cggcatcaaa
    63421 aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac t
//
LOCUS       DADSZD010000005        63461 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.005, whole genome shotgun sequence.
ACCESSION   DADSZD010000005 DADSZD010000000
VERSION     DADSZD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.005"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(110..2044)
                     /locus_tag="J1Z28_000245"
     CDS             complement(110..2044)
                     /locus_tag="J1Z28_000245"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBA7715119.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTFSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(2112..3239)
                     /locus_tag="J1Z28_000246"
     CDS             complement(2112..3239)
                     /locus_tag="J1Z28_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBA7715120.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDPLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESLRWRSSYEV
                     EKLPEEHTKSSTAELCQLANTLSLHPISLSLLEKLLNSTRVNTQPGNLLAALLCARIN
                     GSPACFAACMNSSNEYKKISPLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            complement(3384..4172)
                     /gene="fabI"
                     /locus_tag="J1Z28_000247"
     CDS             complement(3384..4172)
                     /gene="fabI"
                     /locus_tag="J1Z28_000247"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBA7715121.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            4650..5216
                     /locus_tag="J1Z28_000248"
     CDS             4650..5216
                     /locus_tag="J1Z28_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7715122.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            5365..6486
                     /locus_tag="J1Z28_000249"
     CDS             5365..6486
                     /locus_tag="J1Z28_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBA7715123.1"
                     /translation="MKYIATSVVAMLLLSGCDNTQSNNSSPSETEVGVVTLKSQPVSV
                     VSELTGRTSAALSAEVRPQVGGIIQKRLFKEGDLVKAGQPLYQIDAASYQAAWNEARA
                     ALQQAQALVKADCQKAQRYARLVKENGVSQQDADDAQSTCAQDKASVEAKKAALETAR
                     INLDWTTVTAPISGRIGISSVTPGALVTASQDTALTTIRGLDTMYVDLTRSSVDLLRL
                     RKQSLATNSDTMSVSLILEDGTTYSEKGRLELTEVAVDESTGSVTLRAIFPNPQQQLL
                     PGMFVRARVDEGVMENAILAPQQGVTRDAKGNATALVVNKDNKVEQRTLETGETYGDK
                     WLVLNGLHNGDRLIVEGSAKVTSGQTVKAVEVQANGGNA"
     gene            6486..9574
                     /locus_tag="J1Z28_000250"
                     /pseudo
     CDS             6486..9574
                     /locus_tag="J1Z28_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309891.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
     gene            9597..10970
                     /locus_tag="J1Z28_000251"
     CDS             9597..10970
                     /locus_tag="J1Z28_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBA7715124.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            10979..12142
                     /locus_tag="J1Z28_000252"
     CDS             10979..12142
                     /locus_tag="J1Z28_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HBA7715125.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMILLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSCIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(12190..12996)
                     /gene="sapF"
                     /locus_tag="J1Z28_000253"
     CDS             complement(12190..12996)
                     /gene="sapF"
                     /locus_tag="J1Z28_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBA7715126.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(12998..13990)
                     /gene="sapD"
                     /locus_tag="J1Z28_000254"
     CDS             complement(12998..13990)
                     /gene="sapD"
                     /locus_tag="J1Z28_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBA7715127.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            complement(13990..14880)
                     /gene="sapC"
                     /locus_tag="J1Z28_000255"
     CDS             complement(13990..14880)
                     /gene="sapC"
                     /locus_tag="J1Z28_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBA7715128.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            complement(14867..15832)
                     /locus_tag="J1Z28_000256"
     CDS             complement(14867..15832)
                     /locus_tag="J1Z28_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antimicrobial peptide ABC transporter permease
                     SapB"
                     /protein_id="HBA7715129.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            complement(15829..17472)
                     /gene="sapA"
                     /locus_tag="J1Z28_000257"
     CDS             complement(15829..17472)
                     /gene="sapA"
                     /locus_tag="J1Z28_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBA7715130.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEVRLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            complement(17785..18030)
                     /locus_tag="J1Z28_000258"
     CDS             complement(17785..18030)
                     /locus_tag="J1Z28_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBA7715131.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            complement(18164..18271)
                     /locus_tag="J1Z28_000259"
                     /pseudo
     CDS             complement(18164..18271)
                     /locus_tag="J1Z28_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine importer"
     gene            complement(18455..19432)
                     /gene="pspF"
                     /locus_tag="J1Z28_000260"
     CDS             complement(18455..19432)
                     /gene="pspF"
                     /locus_tag="J1Z28_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBA7715132.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            19599..20267
                     /gene="pspA"
                     /locus_tag="J1Z28_000261"
     CDS             19599..20267
                     /gene="pspA"
                     /locus_tag="J1Z28_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBA7715133.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREIEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLVRMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            20321..20545
                     /gene="pspB"
                     /locus_tag="J1Z28_000262"
     CDS             20321..20545
                     /gene="pspB"
                     /locus_tag="J1Z28_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBA7715134.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            20545..20904
                     /gene="pspC"
                     /locus_tag="J1Z28_000263"
     CDS             20545..20904
                     /gene="pspC"
                     /locus_tag="J1Z28_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBA7715135.1"
                     /translation="MAGINLNKKLWRIPQRGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            20913..21134
                     /gene="pspD"
                     /locus_tag="J1Z28_000264"
     CDS             20913..21134
                     /gene="pspD"
                     /locus_tag="J1Z28_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBA7715136.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            21209..21523
                     /gene="pspE"
                     /locus_tag="J1Z28_000265"
     CDS             21209..21523
                     /gene="pspE"
                     /locus_tag="J1Z28_000265"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBA7715137.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            21734..23413
                     /locus_tag="J1Z28_000266"
     CDS             21734..23413
                     /locus_tag="J1Z28_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HBA7715138.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYPQWLQRIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGEGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPENEILALVEVLQQEGALVNWKNNPDGTRSPYEMNVTYMDALS
                     RRECSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     SSKEITTELNNKATLSYSVYYKLSHLITLRRSHKEFHPDNNFTIDAINSFVMRIQRSN
                     ADGDCLTGLFNVSENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            23427..24719
                     /locus_tag="J1Z28_000267"
     CDS             23427..24719
                     /locus_tag="J1Z28_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7715139.1"
                     /translation="MIKSKIVLLSALVSCTLISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIELH"
     gene            24740..25621
                     /locus_tag="J1Z28_000268"
     CDS             24740..25621
                     /locus_tag="J1Z28_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBA7715140.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            25608..26450
                     /locus_tag="J1Z28_000269"
     CDS             25608..26450
                     /locus_tag="J1Z28_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBA7715141.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALLVVIMYALSEHFIKSGLTAG
                     GVKG"
     gene            26481..27533
                     /locus_tag="J1Z28_000270"
     CDS             26481..27533
                     /locus_tag="J1Z28_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBA7715142.1"
                     /translation="MKKLVATAPRVAALVEYEERAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFINDDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            27552..28340
                     /locus_tag="J1Z28_000271"
     CDS             27552..28340
                     /locus_tag="J1Z28_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA7715143.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KVAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            28350..29405
                     /locus_tag="J1Z28_000272"
     CDS             28350..29405
                     /locus_tag="J1Z28_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7715144.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTAEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            29402..31669
                     /gene="ycjT"
                     /locus_tag="J1Z28_000273"
     CDS             29402..31669
                     /gene="ycjT"
                     /locus_tag="J1Z28_000273"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBA7715145.1"
                     /translation="MIRPVTLTEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEIFSLSREAWQRELNFASGELR
                     RNVVWRTSNGAGYTITSRRFVSADQLPLIALEITITPLDADALVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTC
                     AQLHAGETLTLQKRVWIDWRDDRQAALDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWRTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALNIARQFGCSDDA
                     FIHRAEMYLKELLLPEIQPDGVLPQDDSFMAKPVINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLIYVAEESVFCLGDF
                     ILPFNGTATTYQEGE"
     gene            31666..32325
                     /gene="pgmB"
                     /locus_tag="J1Z28_000274"
     CDS             31666..32325
                     /gene="pgmB"
                     /locus_tag="J1Z28_000274"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBA7715146.1"
                     /translation="MKLQGVIFDLDGVITDSAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIEAINASGMRSVGIGAGLTGAQLLLPSTDSLTWPRLSAFWQN
                     V"
     gene            32339..33421
                     /locus_tag="J1Z28_000275"
     CDS             32339..33421
                     /locus_tag="J1Z28_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7715147.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAREVIDERVNWAAQILGLREYLKRKPDALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            33466..34371
                     /gene="ompG"
                     /locus_tag="J1Z28_000276"
     CDS             33466..34371
                     /gene="ompG"
                     /locus_tag="J1Z28_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBA7715148.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDRDKFHYAGVGVNYSF"
     gene            complement(34422..35420)
                     /locus_tag="J1Z28_000277"
     CDS             complement(34422..35420)
                     /locus_tag="J1Z28_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HBA7715149.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTDALIESGCKNIACLHTPLDVHVSVDRVNGYKQSLATHNIAVRD
                     EWIVDGGYTHETALKAARELLSQSPLPEAVFATDSLKLMSIYRASAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            35576..36973
                     /locus_tag="J1Z28_000278"
     CDS             35576..36973
                     /locus_tag="J1Z28_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBA7715150.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYFSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            36970..38031
                     /locus_tag="J1Z28_000279"
     CDS             36970..38031
                     /locus_tag="J1Z28_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBA7715151.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            38179..39720
                     /gene="tyrR"
                     /locus_tag="J1Z28_000280"
     CDS             38179..39720
                     /gene="tyrR"
                     /locus_tag="J1Z28_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBA7715152.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSTLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(39764..40270)
                     /gene="tpx"
                     /locus_tag="J1Z28_000281"
     CDS             complement(39764..40270)
                     /gene="tpx"
                     /locus_tag="J1Z28_000281"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBA7715153.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            40389..41354
                     /gene="ycjG"
                     /locus_tag="J1Z28_000282"
     CDS             40389..41354
                     /gene="ycjG"
                     /locus_tag="J1Z28_000282"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBA7715154.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(41329..42057)
                     /gene="mpaA"
                     /locus_tag="J1Z28_000283"
     CDS             complement(41329..42057)
                     /gene="mpaA"
                     /locus_tag="J1Z28_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBA7715155.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANCVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(42348..42986)
                     /locus_tag="J1Z28_000284"
     CDS             complement(42348..42986)
                     /locus_tag="J1Z28_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7715156.1"
                     /translation="MKNILILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(42983..43057)
                     /locus_tag="J1Z28_000285"
     CDS             complement(42983..43057)
                     /locus_tag="J1Z28_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715157.1"
                     /translation="MKHIQIRNSDMAWHIAANNLGKVS"
     gene            complement(43057..43977)
                     /locus_tag="J1Z28_000286"
     CDS             complement(43057..43977)
                     /locus_tag="J1Z28_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7715158.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSDARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSKDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            44115..45014
                     /locus_tag="J1Z28_000287"
     CDS             44115..45014
                     /locus_tag="J1Z28_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7715159.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            45351..46964
                     /locus_tag="J1Z28_000288"
     CDS             45351..46964
                     /locus_tag="J1Z28_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7715160.1"
                     /translation="MKHSVSVTCCVLLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDSRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNDPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(47015..48046)
                     /gene="ynaI"
                     /locus_tag="J1Z28_000289"
     CDS             complement(47015..48046)
                     /gene="ynaI"
                     /locus_tag="J1Z28_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBA7715161.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGDRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVTEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            48290..48547
                     /locus_tag="J1Z28_000290"
     CDS             48290..48547
                     /locus_tag="J1Z28_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBA7715162.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(48597..49547)
                     /gene="uspE"
                     /locus_tag="J1Z28_000291"
     CDS             complement(48597..49547)
                     /gene="uspE"
                     /locus_tag="J1Z28_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBA7715163.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(49699..50451)
                     /locus_tag="J1Z28_000292"
     CDS             complement(49699..50451)
                     /locus_tag="J1Z28_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FNR family transcription factor"
                     /protein_id="HBA7715164.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(50646..51161)
                     /gene="ogt"
                     /locus_tag="J1Z28_000293"
     CDS             complement(50646..51161)
                     /gene="ogt"
                     /locus_tag="J1Z28_000293"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBA7715165.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKESYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(51172..52698)
                     /gene="abgT"
                     /locus_tag="J1Z28_000294"
     CDS             complement(51172..52698)
                     /gene="abgT"
                     /locus_tag="J1Z28_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HBA7715166.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASYVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQHFGLRIAGVVSLLFIASI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRHQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            complement(52732..54177)
                     /locus_tag="J1Z28_000295"
     CDS             complement(52732..54177)
                     /locus_tag="J1Z28_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBA7715167.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPISATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYEQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFSTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMADVQHIYDRVSK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEEQAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFTLRHRETVLANEVAPYATTDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFLDSGL
                     LQECQQEHQQVTDTQPYHCPIPKSVTPSPLK"
     gene            complement(54177..55487)
                     /locus_tag="J1Z28_000296"
     CDS             complement(54177..55487)
                     /locus_tag="J1Z28_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBA7715168.1"
                     /translation="MDFLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAAFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEGRDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQRHQGQAS
                     YMVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            55663..56571
                     /locus_tag="J1Z28_000297"
     CDS             55663..56571
                     /locus_tag="J1Z28_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7715169.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDICQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQART
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            56901..57464
                     /gene="smrA"
                     /locus_tag="J1Z28_000298"
     CDS             56901..57464
                     /gene="smrA"
                     /locus_tag="J1Z28_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBA7715170.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPARNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(57485..58717)
                     /gene="dgcM"
                     /locus_tag="J1Z28_000299"
     CDS             complement(57485..58717)
                     /gene="dgcM"
                     /locus_tag="J1Z28_000299"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBA7715171.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTRLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            58972..59955
                     /gene="zntB"
                     /locus_tag="J1Z28_000300"
     CDS             58972..59955
                     /gene="zntB"
                     /locus_tag="J1Z28_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBA7715172.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            60433..61806
                     /gene="dbpA"
                     /locus_tag="J1Z28_000301"
     CDS             60433..61806
                     /gene="dbpA"
                     /locus_tag="J1Z28_000301"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBA7715173.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVSLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(61935..62870)
                     /gene="ttcA"
                     /locus_tag="J1Z28_000302"
     CDS             complement(61935..62870)
                     /gene="ttcA"
                     /locus_tag="J1Z28_000302"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBA7715174.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(62922..63152)
                     /locus_tag="J1Z28_000303"
                     /pseudo
     CDS             complement(62922..63152)
                     /locus_tag="J1Z28_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            63194..63319
                     /locus_tag="J1Z28_000304"
                     /pseudo
     CDS             63194..63319
                     /locus_tag="J1Z28_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
BASE COUNT        15781 a        15347 c        16601 g        15732 t
ORIGIN      
        1 aaaaacgcca gttttcaaac gaaagtcagt taaaaatctg ccgggatatg acgaaggttg
       61 agcggggaaa taaacggccc atccatgagg aatgggccgt gaaaggagat tacgcgactg
      121 ggcgcgcaat aatgctgcgg gtttccatgc ggacttcggc aatggtgacg tcaataacgt
      181 cagtcacttt gtaagccgtt tcacctttaa tttgtacagt gccgttttcc tggctgcaaa
      241 ccagttcatc gcgcacagcg tgtaagaaag gtgctggaat aaaggcgata gcgccgttat
      301 caaccaaacg aacacgcatg ccgccacggc tgatatcgac aatttccgcc gcgaaacggg
      361 tgtcggtccc ggctttgtct ttcaggaagc gtgcgtataa ccagtcgcca acatcacgtt
      421 ctgccatccg gttgagacgg cgacgctcgg ccatttggac agtgatttca tcctgtggac
      481 gcgtcgcagt ttcgcctttg ataaccgctt tcagcagacg gtggttgatc atgtcgccat
      541 atttacggat cggcgaagtc caggtggcgt atgcttccag accgaggcca aagtgaggac
      601 cgggttcagt gctaatttca gcaaatgact ggaagcgacg aatgcggctg tcgaggaaac
      661 cagttggttg cgcatccagt tcacgacgca gtttgcagaa accgtccagc gtgagcactt
      721 cttcagcatc gacatgcaga ccgtgcgttt tcagcaacgc tgccagcgcg tcggcattcg
      781 ccggatcaaa gcccatatgc acgttataga tgccaaaacc gagcttatcg cgcagtacgc
      841 gggccgcaca aatgttagcg gcaatcatcg cttcttcgac gatacggttg gcaatgcgac
      901 gaggctcggc gacgatatcc agcacttcac ctttttcacc gagaataaag cggtaatccg
      961 ggcgatcttt aaacaccagt gcgtggttat gacgccactc gccgcggcgt tggcaaattt
     1021 gtgctagcaa acggacttgt tcggcaattg cttcgctttc aggctgccag tcaccggtat
     1081 tttccagcca gtcagaaacc tggtcataca ccagcttcgc tttggattcg atggtggcgg
     1141 caaagaattc gatattatct tcaatggtgc catcagtgga gaacgtcatg cggcatgcca
     1201 gtaccgggcg gacttcattg gcgcgcagtg agcagagatc gtcagaaagc tcgcgaggca
     1261 gcatagggat gttgaagcca ggcagatagt tggtgaatgc gcgaattttc gcggctttgt
     1321 ccagcttgct gccttcagca atccacgcgg ttggatcggc aatcgccaca atcagctgaa
     1381 gtttgccatc cggtaacgcc ttggcgaaaa gggcgtcatc catatcttct gtgctggcac
     1441 tgtcgatggt gacaaaatcc agcgcagtca gatcttcacg aaccagacct tcatcgagca
     1501 tttcggtagc gacgccgtct ggtgcttctt tttccagatt atgacgcgca agggtaaccc
     1561 accacggtac aaagtgatcg tcaccaaaag tgatgtattg tgtcagttca gcatagaaag
     1621 aacgatcgcc tttcagcgga tgacggcgca tttcggcaac cgcccagtcg ccttctttaa
     1681 actcgtggtt caggccacgg gctgcgcggc aaggaatggc gtcttttaag agtggatgat
     1741 caggaacgat ggccagacgg tcatttttgc cctgaacttt acccacgaaa cgagtcagga
     1801 acggttcaac cagttcttct ggctctgcgg attcacgttc tttttcactg tggatcaccg
     1861 cgataattcg gtcgccatgc atgacttttt tcatctgcgg cggcggaatg aaataacttt
     1921 tttgcgcgtc gacttccagg aagccaaagc ctttttctgt ggcttttacc accccttcag
     1981 cgcgtggcgt ctgggaatgc agttgctgtt taagctgcgc tagcagcggg ttgtcctgaa
     2041 acataattgt ctattttggt ggccattgga gcggctgaca gttttacgcg aatctgtctg
     2101 acgcggcaag gttaatatgt ctcacccaac gcgattttta agcgatttat ccagccacac
     2161 agcccgctcc ataccagcag attaataatc tgcgttgatg atttttcgtg ttcgagtaat
     2221 gggctgaact gggcggcgct aaagcgatcg ggtgctcggg tcagccattg tatcgcctga
     2281 acggtagcgc gctcaacaga atgacggtca gcccatcggt taatttcatt ttcgcccttg
     2341 cgcagaaggg ggctgatttt tttatattca tttgatgaat tcatacaggc ggcaaaacaa
     2401 gcaggactgc catttatccg cgcgcataac aacgccgcaa gcaggttacc cggctgtgta
     2461 ttgacccgtg tgctgtttaa caacttttcg agaagggaaa gtgaaatagg gtggagagag
     2521 agtgtgttgg ccagttggca aagttctgca gttgaacttt ttgtgtgctc ttcagggagt
     2581 ttttccacct catagctgct tcgccagcgt aatgattcat cagcaaacag tgacgcgtcg
     2641 gcgtagtttt gtatctccag cccgggtaac cagcgtactg gatgcccgag ataagcctga
     2701 aatgtcgcaa ttgtccgcgc ctgaaagcca ataaaaccaa tgatttggtt aatgatcaca
     2761 cagtcacaga cagtaagacc aacgtcattc agatgctggc gcgatcggtt gtcgataata
     2821 gaaggcgagc tagccagttg gcgggcatat tgcgtgattt gtgccaaccg atgattactt
     2881 tccctggagg aatcaggccc ggggagcggc gtaagtcgcg ccgaatagta gttacataac
     2941 cgttggacgc cgcaaacctg agcaaccgtc agggccgtcg acaagcgttc ataagcggtg
     3001 aaggtctgtg aacgggtcac ggtaagcggg tcagggaaca actctactgc aagaacgcgc
     3061 gcagggacca gccatgaaag gaagggtgcc agcgtttctt catcaaggat cacgtcgagc
     3121 agaaagggat cgctgacctt tgcggcttcc gggaccagag gcagaacgtc atactccgca
     3181 gtactggatt gcgtttcatg ataccagtgg cttttgccgg tgatgtggcg ttgttccatg
     3241 ggcgctcctt ggtcgtaaag gaaatcgtta tcctgacgca aggcgggaag gggagaaaga
     3301 cggatcggcg ataacaaata tcagaaaggt ataacagaga taacgggcgg cagaacgccg
     3361 cccaatattt accaacagaa cgattatttc agttcgagtt cgttcattgc agcgatgctg
     3421 aaaccgccgt caacgtggac cacttcaccg gagataccgg cagagagatc ggagcacagg
     3481 aatgccgcgg aattacccac atcttcaata gtaacggtac ggcgaatcgg ggtaacggct
     3541 tcgcaatgag ccagcatttt gcggaagtct ttgataccgg aagccgccag agtacggatc
     3601 ggacccgcag agatggcgtt aacacgcaca ccttccggac ccatcgcgtt cgccatatag
     3661 cgcacgttcg cttccagaga cgcttttgcc agacccataa cgttgtagtt cgggatagcg
     3721 cgctcagcgc caaggtagga aagggtcagc agggcagaac ccggattcag catggagcgg
     3781 caagcttttg ccattgcaac gaagctgtag gagctgatgt cgtgggcaat tttgaagcct
     3841 tcacgggtaa cggcgttaac atagtcacca tccagctgat cgccaggtgc aaaaccaata
     3901 gagtgtacga aaccgtcaaa tttcggccaa actttcccca gttcagcgaa catggtgtcg
     3961 atgctggcat cttcggcaac atcgcactgc agaacgatgt cagaacccaa ttgagcggca
     4021 aattcttcta cgcggccttt cagtttgtcg ttctggtagg tgaatgccag ttcagctcct
     4081 tcgcggtgca tcgcctgagc gataccgtag gcgatggaca gtttgctggc aacaccggtt
     4141 accagaatgc gcttaccgga aagaaaaccc atagctttaa tccttattgt tgatgcttgt
     4201 tgtgcctgaa aatcaggcga ttcgtcgttt tagtaaacag tacgaacaga taaacggtta
     4261 ttataatcaa cctggctgtg ggtggctata gttgcgcttt ctgtagcgga gagaagctgc
     4321 ggtacagagc attttgcgtt atctactctt gacgcctgca aggggtaggt tggggaaata
     4381 tcttatagaa tctgactatg aaactattcg tattagctcc gataagtttg atgatgaaaa
     4441 cagaactggc gacaacaagt cgctgccttt ctgatgaatt gaccccaact taatattgtc
     4501 aactatcttc acagaatctc cacaatctct tcattatcag aatatgcacc atgaataaga
     4561 taaaaatact ttccattact ttggagaaac gtataatcag caaaaatgag aacgatcatt
     4621 ctcgccaagt gataggccag gagacagaaa tgaccagcaa gctggagata cgccacaaac
     4681 agcgtcagga tgaaatcatt aacgccgccc gtcggtgttt tcgtcgctgc ggatttcatg
     4741 ccgccagtat gtcgcaaatt gccagcgaag ctcagcttag cgtgggtcag atatatcgtt
     4801 acttcgccaa caaagacgcc attattgaag agatggttcg tcgtatcatc gactttcgta
     4861 ttgcccaaat ggacattgac gcgcgtaccg atcacttccc ggaagtcctt gccttgcgta
     4921 aatccttaaa tgaggacgac gacgcgctga tgctggaagt ggctgcagaa gccacacgca
     4981 acccgcgggt tatggcaatg cttgaagaag ctgacgccag aatgttcgct aacggttgtg
     5041 cacatatgaa aagaatgcat ccccatctta gcgacgagca cattcgttgc tgcgttgaag
     5101 tgtttgctac catgatggaa ggtacggttt atcgtcgttt aacaccgcaa aaaagcgatc
     5161 cacaacatct gcaggaaata tatcaggata ttgttagtat gttgataaat aaataagaac
     5221 agaattaacc aatagcgggg gatattaatc ccccttattt cattgcgaaa ttcatttcgc
     5281 gatgcgggtt cgttataact gaatatccct gacatatcct cattaatttc aatgaggagg
     5341 gtatttctca ccctggagaa aataatgaag tatatagcaa catctgtagt ggcaatgctg
     5401 ctcttatcgg gttgtgataa tacgcaaagt aacaattcat ccccgtcaga aacagaagtc
     5461 ggcgttgtta cgctcaaatc tcaaccggtt tcggtagtca gtgaattaac cggacgcacc
     5521 agtgctgcgc tcagtgctga agtacgtccg caggttgggg gaattatcca gaaacgctta
     5581 tttaaggaag gtgatctggt caaggctgga cagcctctct accagattga tgcggccagt
     5641 tatcaggctg catggaatga agcccgggca gcattacaac aagcacaggc actggtaaaa
     5701 gccgattgcc agaaagcgca gcgttatgcc cgactggtga aagagaacgg tgtttcacaa
     5761 caggatgctg atgatgctca gtctacctgt gcacaagata aagccagtgt agaggcgaaa
     5821 aaagccgcac tggaaactgc gcgcattaat cttgactgga ccacggtaac cgcaccgatt
     5881 tcggggcgta ttggcatttc gtcggtaacc cctggcgcac tggtgaccgc gtcgcaggat
     5941 acagcgttaa cgactattcg tggtctggat acaatgtatg tcgacctcac tcgctccagt
     6001 gtcgatttat tacgtctgcg taaacagtca ctggcgacca acagtgacac tatgagcgtc
     6061 tcacttattc tggaagatgg cacaacctac agcgaaaaag ggcgtctgga actcaccgaa
     6121 gtcgcggtgg atgagtctac cggttcggtg acattacggg caattttccc caatccacaa
     6181 cagcagttat taccgggaat gtttgttcgc gctcgtgtcg atgaaggcgt gatggaaaac
     6241 gctattctcg cgcctcaaca gggcgttacg cgcgatgcta aaggcaatgc aactgcgctg
     6301 gtggtgaata aagacaataa agtagagcag cgaacgctcg aaacgggaga aacgtatggt
     6361 gataaatggc tggtgctgaa cggcctgcac aacggcgacc gactgattgt tgaaggttct
     6421 gccaaagtca cttcagggca gaccgtcaag gctgttgaag ttcaggctaa tggaggcaac
     6481 gcctgatgtt ttcgcgcttt tttgttcgcc gcccggtctt tgcctgggtt atcgccattt
     6541 tgattatgtt ggcggggatt ctggctattc gcacattgcc cgtcgcgcaa tatcctgacg
     6601 ttgcaccgcc gaccattaaa atttcagcca cttatactgg tgcttctgcc gaaacgctgg
     6661 agaacagcgt gactcaggtt atcgagcaac aactcactgg gcttgataat ttactctatt
     6721 tcagctcaac cagtagctct gatggttcgg tcagtattaa tgtgaccttt gaacaaggta
     6781 ccgatccaga tactgcacag gttcaggtac agaataaaat tcagcaggcg gagtcgcgcc
     6841 tacccagcga agtgcagcaa acgggtgtta cggtggagaa atcacaaagc aactttttgc
     6901 tgattgctgc cgtgtatgac accactgaca aagcctccag ttcggatatc gccgactggc
     6961 tggtcagtaa cgttcaggac ccgctggcgc gtgttgaagg tgtggggagt ctgcaagtct
     7021 ttggcgcgga atacgctatg cgcatctggc ttgacccggc caaactggcg tcttactcgc
     7081 tgatgccttc agacgtgcaa agtgctattg aagcgcaaaa cgtgcaggtt actgccggga
     7141 aaataggggc attgccttca ccgaatactc agcaactgac cgcaacggta cgtgcgcagt
     7201 ctcgtttgca gacggtggat cagttcaaaa atattatcgt gaaaagccag tcagacggcg
     7261 cagttgttcg tataaaagat gtggctcgcg ttgagatggg cagtgaagat tataccgcta
     7321 tcggcaaact taacggtcac ccgtctgccg gggttgctgt aatgctttcg cccggtgcga
     7381 atgcgctgaa tacggcgacg ctggtcaagg ataagattgc cgaattccag cggaacatgc
     7441 cgcagggata cgacatcgca tacccgaaag acagcactga atttattaaa atctccgtag
     7501 aggacgtaat tcagacgctg tttgaagcca tcgtattggt ggtttgcgtg atgtatttat
     7561 tcctgcaaaa cctgcgtgcc acactgattc cggcgttagc tgtccccgtc gttttgctgg
     7621 gcacattcgg cgttcttgcg ttgtttggct attcgattaa taccctgacg ttgtttgcaa
     7681 tggtgctggc gatcggcttg ctggtggacg atgccattgt ggtggtagag aacgtcgaac
     7741 gcattatgcg tgacgaaggg ttacccgcgc gtgaagccac ggaaaaatca atgggcgaga
     7801 tttctggcgc actggttgcc attgcgctgg tgttgtcagc cgtattcctg ccgatggcct
     7861 tctttggggg atctacgggg gtaatttatc gtcagttctc catcaccatc atctccgcaa
     7921 tgctgctttc cgtggtggtg gcattgacct tgactcccgc cctgtgcggt tccgtcctcc
     7981 agcatgttcc gccacataaa aaaggctttt tcggcgcatt taaccgcttt tatcgccgta
     8041 ctgaagataa atatcagcga ggcgtaattt atgtcctgcg ccgtgcagcc cgaacgatgg
     8101 ggctttatgt cgtgctgggt ggtggaatgg ccctgatgat gtggaaactg ccgggcagtt
     8161 tcttacccac tgaagaccag ggcgaaatca tggtgcagta cacgctgccg gccggggcga
     8221 ccgctgcccg tacagcagaa gtgaatcgcc agattgttga ctggttcctg attaacgaga
     8281 aagcaaatac cgatgtcatc tttaccgttg atggtttcag ttttagcggc agcggacaga
     8341 acaccgggat ggcgtttgtt tcgttgaaaa actggtctca acgtaaaggg gcagaaaaca
     8401 ccgcccaggc tatcgcccta cgggctacca aagagctggg cacaattcgt gatgccacgg
     8461 tatttgcgat gacgccgcca gccgttgatg ggctgggcca aagcaatggt tttacgtttg
     8521 aattgttagc taacggtgga accgatcgtg aaacactgct gcaaatgcgt aatcagttga
     8581 tagaaaaagc gaatcaaagt ccggagttac attctgtacg cgccaatgat ttgccacaaa
     8641 tgccgcaatt gcaggtagat attgatagca ataaagcggt gtcattaggg ttgagtttga
     8701 atgatgtcac cgacaccctg tccagtgcgt ggggcggtac ttatgtgaat gactttattg
     8761 accgtggtcg tgtgaaaaaa gtctacattc agggcgacag cgaatttcgt tccgcgccgt
     8821 cagacttagg taaatggttt gtgcgcggta gcgataacgc catgacacca ttctctgctt
     8881 ttgcgaccac ccgctggctg tatggaccgg aaagactggt gcgctacaac ggctcggcag
     8941 cctatgaaat tcagggcgaa aacgcgactg gctttagttc cggcgatgca atgacaaaaa
     9001 tggaggaact ggcaaacagt cttcctgcgg gaacaacctg ggcctggagt ggtttgtcat
     9061 tgcaggagaa actggccagc ggtcaggcat taagtctgta tgcagtttct attctggtgg
     9121 ttttcctctg ccttgcagca ctgtacgaaa gctggtcagt cccgttctcg gtcattctgg
     9181 tgatccctct ggggcttctt ggcgcggcgc tggcggcctg gatgcgtgat ttaaacaacg
     9241 acgtttactt ccaggtagcg ctattaacta ctatcggctt gtcgtcgaaa aacgccattc
     9301 tgattgtgga atttgctgaa gcggcggttg cagagggcta ttccttgagt cgtgcggcat
     9361 tacgcgcggc gcagactcgt ttacgcccaa tcatcatgac ctcgctggcg tttattgcgg
     9421 gggtaatgcc gctggcgata gcaaccggcg caggggcgaa cagccgcatc gccattggta
     9481 cgggcattat tggcggcacg ctgaccgcta cattactggc cattttcttc gttcctctgt
     9541 tttttgtact ggtgaaacgt ttgtttgccg gtaaaccgcg ccgtcaggag taagttatgt
     9601 tgcgtcgttc tcttattttt ctggtgttgt tgagtgcggg atgtgtctcg ctcgatcctc
     9661 actacagtac gcctgaatcc ccaatcccgg cgaccttgcc tggagcacag ggccagggga
     9721 aggcaatcag ccatgactgg cagcaggtga tccacgatcc ccgattgcag caggtagtaa
     9781 ccattgcact gaacagtaac cgcgatgtgc aaaaagcgat tgctgatatt gactcagcac
     9841 gggcgctcta tgggcaaact aatgcgtcgt tatttccgac ggtgaatgcg gccttaagtt
     9901 cgactcgcag tcgttcgctg gcgaatggta cgggaacaac tgccgaggct gacggcacgg
     9961 tgtccagtta tacgctagat ctgtttggtc gtaatcagag tttatctcgg gcggcgcggg
    10021 aaacctggct tgccagtgaa tttacagccc agaacactcg tttaacgctg attgctgaaa
    10081 tcagcaccgc ctggctgaca ctggcggccg ataacagcaa cctggccctg gcaaaagaaa
    10141 cgatggccag tgcagaaaac tcattgaaaa ttatccagcg ccagcaacag gttggcacgg
    10201 cggcagcgac agatgtcagt gaggcgatga gtgtttatca gcaagcgcgc gccagcgttg
    10261 ccagctatca gacacaagtg atgcaggata aaaatgcgct gaacttactg gcaggtacaa
    10321 cgcttgcgga aaatctgcta ccgggaacac tggaaagcct gccggaacaa atgataagtc
    10381 tggttcctgc gggagtatca tctgatgttc tgctgcgtcg cccggatatc caggaagcgg
    10441 aacataattt gaagagcgcc aatgcagata ttggcgcggc gcgggctaac tttttcccga
    10501 cgatttcgct gactgccagt gccggtgttg gcagtgacgc attgtcgtcg ttgttcagcc
    10561 acggaatgca gatctggtca tttgcgccat cggtcacatt accgctgttc actggcggaa
    10621 gtaatctggc gcagcttcgt tacgcagaag cacaaaagcg tgggctcatt gccacctatg
    10681 aaaaaaccgt tcagagcgca tttaaagatg tcgctaacgc actggcgcgg cggaccacgc
    10741 ttgaagagca actggatgca cagcgccagt atgttaaagc tgagcaacaa acggtcgatg
    10801 ttggcttgcg tcgctatcag gcgggtgttg gtgattacct gaccgtgttg acggcgcagc
    10861 gcagcttgtg gagtgcacag caggagctgc tggcactgca actgactgat tttacaaacc
    10921 gaatcacgct ctggcagtcg ctgggcggcg gtatgtcatc actcaagtaa ggaataatat
    10981 ggctagagtc tctctttcat gggcattgat tcttggtctt ttagccggta tcggcccgat
    11041 gtgtaccgat ctttatttgc cggctttgcc ggagatgtca gagcaactgg cggcaaccac
    11101 gaccataacg caattaaccc tgaccgcatc actgattggt cttggcgtcg gacaactgtt
    11161 atttggccct ctgagcgaca aaatagggcg taaacgtccg ttaatcttgt cgttgctatt
    11221 gtttattgtt tcttccattt tatgcgcgac aacgaacaat atttactggc tggtggtctg
    11281 gcgttttatt caagggatcg cgggggcggg tggttcggtg ctctctcgtt ctattgctcg
    11341 tgacaaatat cagggagtaa cgttgaccca gttttttgcg ctgttaatga cggtgaatgg
    11401 cctggcaccg gtgttgtcgc cagtgctggg cgggtacatt gttagcactt ttgactggcg
    11461 cactttattc tgggtaatgg ctgaaattag caccgtactg ttgctgggct gcctgttatt
    11521 tattaatgag accttgccag aaaataaaag gggctcatca ttgctattaa ccggacgaag
    11581 cgtggtgcag aaccgccgtt ttatgcgctt ttgcctgatt caaagtttta tgctggctgg
    11641 tttgtttgca tatatcggct cttcgtcgtt cgtgttgcag aaggaatttg gctttagtcc
    11701 aatgcaattt agcctggtgt ttggccttaa cggcatcgga cttatcattg cttcatggat
    11761 cttctcgcgc ctggcgcgac ggattaacgc gatgatattg ttgcgaggtg gcctgatagc
    11821 ggcaattttg tgtgcattgc tcacggtctt atgcgcatgg gtacaattgc ccattccggc
    11881 actggtggca ttatttttca ccatcgcatt ttgtagctgc atcggcactg ttggcggggc
    11941 agaggctatg agtgcagtag ggacgcagga atctggaacg gcgtctgcgt tgatggggat
    12001 gagcatgttt gtcttcggcg gtatagccgc gccattgtcg ggaattggcg gagaaacact
    12061 gttaaaaatg agtctggcaa ttacggtgtg ttatacgctg gcattgctgg ttgctctcac
    12121 cagaatcgac aatcaaaagt aatgaacacc tcatcctgag gggggcctgt caggatgagg
    12181 tgcaaaacac taacggtcct tacgccacgc atccgccgtc aatgcttcgc caaaatgacc
    12241 ggcgatcagc cgtttggtga gttcatgcag cggcgatgcc agcacatctg cggtgctgcc
    12301 tcgctcgaca acctcgccct gatgcatcac cagcacctgg tcgctaatgt gcttcatcat
    12361 tccgatatgc tgggtaacat aaatatacga aatgccctgt ttttcctgta actccagcat
    12421 cagattaatc aactgcgaac gcatcgacat atccagtgag gcgagggctt catcggcaat
    12481 aatgactttt gggcgcaata tcagcgcgcg tgccagaccc agacgctgtt tttgcccggg
    12541 tgctaacata tgcggatagt aactgacgtg atccggtagc agcccaacca tacgcatcgt
    12601 ttcaataatc tgtttgcgac gctgttccgg ttccaggtcg gtgttcaggc gcagtggaaa
    12661 atccagaatt tgcgagatac gttgacgggg attcaacgag gtcgaaggat cctgaaaaat
    12721 catgcgaata cgctgactgc ggaaggaata atcgccaaaa tgcagcggat gatcgtcaat
    12781 caataactcg ccgctggtcg gttctatcat tcccgccagc atttttgcca gcgtggattt
    12841 acccgaacca ttctcgccga taatcgccag tgtctggcct tcacgtagcg taaagctcaa
    12901 gggttttacc gcttctacgg tctgacgacg aaaccagccg gtccggtagc ggaacgtttt
    12961 acttaaattg cgcacttcaa gtagcgtttc gatcatctca ctctttctcc atgttcagcg
    13021 ggaaatgaca ggcatagaga tgatttttcg cccccgtcaa acgtggcgtc acaatgcatt
    13081 ctcgttgtgc atacgggcaa cgtggcccca gacgacaacc aatcggtaac tgttccagca
    13141 gcgggatagc gccgggcagc gtattgaggc gacttttatg cggcatcgcg ctgccgaagt
    13201 ctggtatcgc gcggatcagc gcctgggtat aaggatggtg tggcatcgtc accaactcct
    13261 tactcggcgc ggtttccacc gtttgaccgc agtaaagcac gttaatttta tccgcccatt
    13321 ggctaagcat ttgtaagtca tggctgataa gcaaaatagt ggtattgctg ttttggttga
    13381 gacgcgtcag caggcgaaag atttgcgcct gggttgttgg ctccattgag ttggtcggtt
    13441 cgtcagcaat tagcagacgc ggttgattcg ccagtgcaat ggctatcatc actttctgac
    13501 attcaccttc ggtcaactca tagggaaaac tgcgcatcgc atctttgtga tctttaatcc
    13561 ccacgcggtg cagcagttca atggcacggc gtttgcgcca gccaaaacgc tgccaccaac
    13621 ggcctttata ggtccaggca gggatgtttt gcatcaactg gcgacccaca cgttctgaag
    13681 ggtcaagaca cgactgtggt tcctggaaaa tcatcgacac gttgtgacca accagcttgc
    13741 gccgttcgcg tgcggagaga cgcagcaaat cgatatcatc aaaacgcata cggtcagcag
    13801 taacacgcca gttatcttta ttcaccccac aaatcgcttt agcaatcaaa cttttgccgg
    13861 aaccggattc gccaacaaga ccgcggattt caccttcggt taaggtcata cttacgcggt
    13921 cgacggcttt aacccactca tcaccggttt taaattcaat ggtcaggtta cgaatatcga
    13981 gtaatggcat tattccaccc ccgcaataat tgcacgacgg acaccgtcgc caaggaggtt
    14041 aaccaacagc acgctaatca taattgccgc acctggcagc atgacagtcc atggggcgac
    14101 ataaatcagt tccagcgcat caccgagcat cgcgccccat tcaggcgagg gcagttgtgc
    14161 gccgaggtca aggaagccca gcgcggcgat atcgagaatt gccatcgaca atgcgcgagt
    14221 gatctcggtg accagtccgg cggtgatgtt gggcatcaca gcaaaccaga gaatattcag
    14281 cgttgatgcg ccatccagac gggcggcgat aacgtactct ttttccagtt cgtcatgcac
    14341 catgctgtaa atcgaacgta ccatacgcgg cagcaatgcc agccagacag caaacatggc
    14401 gtgagacaaa ctcggtccgg caaacgctac aacgataatt gccagcagca gcgaagggat
    14461 cgccagtaag gtatccagaa tatggttaag caccgctgag cgtaggccgt gggtcgcccc
    14521 ggcgaaggtg cccagtacca ggccacagat cgtcgcggcg agcgtcacca caaatgcgcc
    14581 acctacggtt ggcgcagctc cgctcagtaa acggcttaaa acatcgcgtc cgaggtcgtc
    14641 agtccccagg aagaaagaaa cttcgccata gcgcgaccat gacggcggca ataattgata
    14701 accgagaaat tgctggtcga tgccgtaagg cgcaaaccag ccgccaaaaa tacacagtac
    14761 agccagcccc gcgcagccgt acaggccgac catcgcagag gcgtcactat aaaatttgcg
    14821 ccaggcggta cgcagcgtgc ccggcgggcg cttttcgctg tatacgctat cgtaaggcat
    14881 accattcctt atgtttcaga gggttagcca tggcacccaa aatatcagaa atcacgttaa
    14941 caataatgac cagtgagcca cacaccatca ctccggcgga aatggctgcg taatcctgct
    15001 ggcggatggc gttaattaac cagcgcccta aacccggcca gctaaaaacc atttcggtga
    15061 tcatcgccag cgtcagcatg gtagaaaact gtaagcccag gcgaggaata accggaggta
    15121 acgcgttatg cagaacgtgg cgacgcaaaa tagtaaagcg cgacaaaccg cgggtcgccg
    15181 ctgcttttac ataattctgg tcatacactt cgatagtgct gatacgcatt agccggatca
    15241 cttcagttgt tggcgcaacc gacagggtga tcacgggcaa tatcatatgg cggattgcgc
    15301 tcatgaccat ttcatcccgc cacggtgagt ccgaaagcca ggcatcaatc aacgcaaaac
    15361 cggtaatcgg tttcacttcg tagagcaaat cgaaacgccc agaaacgggc aaccaaccta
    15421 gcgtgagtga acaaaacagc gtcagcagaa gcgccagcca gaacaccggg attgaaaagc
    15481 ccagtaaggc gatggcgttg atcaagttat cctgccactt atggcgcgta atcccggcaa
    15541 tcattcctac ggggatgcca acaatcagcg caaaaccgaa ggcgaggatg cacagttcca
    15601 tcgtcgccgg gaagacctct tttaactgtt cggcaatcgg ttgaccgtta atgctggaaa
    15661 caccaaaatc ccagtggatc aggccgttaa accagaacac ccaggcattc cacagtgacg
    15721 cgccttgtaa cggcgcatgt ggggtgaaat agctcaagct gaagccaacg aaggtcagca
    15781 ggaacaaggt cacaatcaat aacaaaatac ggcgtaaggt gaagataatc atggcttttt
    15841 cacctcatcc tgtttctcgc gatacacccc agcaaaggag gcgttaccaa acgggctaag
    15901 taccagacct ttgatatcat accgataggc ctgcaaacgc aatgacgacg ccagcggcag
    15961 aataggtaat tcctgcgcca gaatactttg cgcttcgtca taggcttcaa tacgcgccgc
    16021 cagctgctgc gaagagagcg ccttacgcaa tacgctgtcg aatttcggat cgcaccagtg
    16081 ggcgaggttg gtctgtgaat gaattgccgc gcagctcagt aacggacgga agaaactgtc
    16141 cgggtcgtta ctgtccgtcg cccaaccgga taacgtcaga tcatggctca tatccatcaa
    16201 ccgcacctcc tgaaaacgac cttctaccgg cacaatcacc acttttacgc caacctgtgc
    16261 catatccgcc tgaatcagtt cggcggtttt cagtggactg gggttccacg cctgcgaacg
    16321 tgtgggcacc cacagtttca gcgttaagtt ttccagcccc aacgccttca actgttcgcg
    16381 cgattttgct ggattgtatt cagtaatttt agcctcgttg tcataggccc acgaggcgcg
    16441 cggtaaaata gaggccgccg tttcagccgt accataatag atggattgca tcaggcgctg
    16501 gttattaatc gccagtgcca gcgcatggcg gaccgccgga ttatttagcg gcggtttggc
    16561 ggtgttgaat gccagatagg cgacattcat cccaggacgc agcgttaaac gcaagcgtgg
    16621 gtcgtcacgc aaaatggata gctgactggc agcaggccag gccagaacgt cgcactcccc
    16681 ggtcaggagt ttcgacaatc gtcctgtacc gccagagcct aaatccacca ccacttgtgg
    16741 catcaacggc ttaccacgcc agaaatcatc atgacgttgt aagcgaatat attgcccggc
    16801 gcggtattcc gacaactgat acggaccggt gccgaccggt tgacggtcga gttgctcctg
    16861 gcgatcttct ttctctaact tccctgcata ttctgccgac atgaccgaag cataatgggt
    16921 cgccaggtgc cacaaaaaag aagcatccgg ctgggcgaga cggaactcaa cggtatgatt
    16981 atccagcttg cggacgcttt tcacgttatc ggcaaattgc agactgtcga aataggggaa
    17041 gttactgccg ttgacgttat gccacgggtt gttgcggtca aaaatccgtt ggaaggtaaa
    17101 caccacatca tcggcattca ttttacgagt gggagtaaac cagtcggttt tttgaaacgg
    17161 aacatcgcga cgcaggtgga agcgataggt cgcgccgttg tcgagtactt cccagctttc
    17221 ggcaagttcc ggcatcaggc gataggtata gggatcgaca tccagcagtc gatcataaaa
    17281 ctgggcggca agggtatcga caattaaccc actgctcgct ttggatgggt taaaggtgtt
    17341 gacttgcccg ctgacgcaat agacaaaacc gctgtcgcgg atatcagcat gcggggggga
    17401 ttcaggcgcg gcgattgcct gaccactcac aagtccagca atcaccaaaa gagacgataa
    17461 tacctggcgc ataatattaa gggattttat gtaaagaggc tatcttacta atatttaatg
    17521 acatttgcca ttaccgtttg tgttcagagg tcgtaatgag ggccgtgtgg ttggtcattg
    17581 aacaggtggc gtcgccatca gttcaacatt cgtacccgtg atttctttat aactataact
    17641 cacagaacaa cttagcgagg agcatagcgg tacgatcgtg agaaacccat taaagcatgc
    17701 tgcagtgcgt gtcattttta gccggatgcg gcgcgaacgc tttatccggg caacgatact
    17761 gaccgatagt ctgcaattaa ataattactc attaccccat tgattcaaaa actctgcgat
    17821 ctcatcaatg cgtacgggat taatccccgc ttcagcagcc atttcatgtt gggcttcttc
    17881 actgatctct tcattattca tcaaacgggt gagcagtaac tggaagtagt gggccagcgg
    17941 tgtgcgttca gcgtctgcga caggctctgc gcattcggtc gcgtactcat cggcaatatc
    18001 aaaatatttt agcgggatat cgtggttcat tatttgcccc tggggttaat gcgctaagca
    18061 taaaggcgga tgatagcact cgtgatccgg gtcatacata tttttatagt taatggcgcg
    18121 gtgcattacc ctcaggcagg ataatgcgcc gcgcattcga ctattacgtt tcgctcaccg
    18181 gcgttctgtc gccatcatac aacggcactt tgcgatagcg gcggatcaaa taccacaaat
    18241 atgcgccgcc taacgaagcc cataccatcc cggttgcgca attcgatgcg gcaatgaaaa
    18301 ttttgcagga tgcgctgaga gattaatacg tagaagcagg gttaacgcaa taatgcctga
    18361 tgcgcagcgc ttatcaggcc tgcaaacgac gtattgattt tgtaggcctg ataaggcgtt
    18421 cacggcacac ccggcaagtt gcattgtcga aatgctaaat ctggtgcttt ttcaacaacg
    18481 cgcggaactg atgataggtt aaccccagca attcagcggc gcgcttctgg ttatatttcc
    18541 cctgttgcaa actgagttgc agcaactctt tttcctgctg catctgaaac tcgcgtaaat
    18601 ccagtggcag cgtcggaagc gaggtgtttt ctgaaacggc gatggcttct tcagatggac
    18661 ggcgtttaaa gggatcaata atgatgtcat caagcggata atcgctggtg ccgtgacgat
    18721 acactgaacg ttccaccacg tttttcaatt cacgtatatt tcccggccag cgataattca
    18781 gcaatgtttc tctggcgtgc tcggtaaaac ccgggaacag aggcagcttg atttcccgac
    18841 acatctggat ggcaaagtgt tctgccatca acataatgtc gctttcacgc tcgcgcagtg
    18901 gtggcagttg cacaacatcg aacgctagtc ggtcgagcag gtcagcgcga aaagtacctt
    18961 cattgaccat cgccgggaga tcggcattcg tcgcgcatac caaccgcaca ttcacctgca
    19021 atggttggct gccgccgacg cgctccagtt caccgtactc aatcacacgc aataattttt
    19081 cctgcaccat catcggtgcc gtagcgagtt catcaagaaa tagcgtaccg ccgtcggcac
    19141 gttcaaatct ccctggatga cgtttttgcg caccggtaaa cgcccccgct tcgtgaccaa
    19201 acagttcgga atccagcaga ttttcattta acgccgcgca gttaagggaa ataaacggcc
    19261 cttgccaacg ggaggagaga taatgcaggc ggctggcgat cagctcttta ccggtgccgc
    19321 gctcgccgat gatgagcacc ggtttgtcca gcggtgcgag atgcgaaacc tgttccagca
    19381 cttcgagaaa gctgttcgcc tcaccgagta aattatcttt gtattctgcc atgatgaaat
    19441 tcgccacttg ttagtgtaat tcgctaattc atcctggcat gttgctgttg attcttcaat
    19501 cagatcttta taaatcaaaa agataaaaaa ttggcacgca aattgtatta acagttcagc
    19561 aggacaatcc tgaacgcaga aatcaagagg acaacattat gggtattttt tctcgctttg
    19621 ccgacatcgt gaatgccaac atcaacgctc tgttagagaa agcggaagat ccacagaaac
    19681 tggtgcgtct gatgatccag gagatggaag atacattggt tgaagtacgt tctacttcgg
    19741 cgcgtgcgct ggcagaaaag aaacagctga ctcgccgtat tgaacaagcg tcggcgcgtg
    19801 aaattgaatg gcaggaaaaa gcggaactgg cactgctgaa agatagagaa gatctggcgc
    19861 gtgcggcgtt aattgaaaaa cagaaactga ccgatctgat taagagcctg gaacatgaag
    19921 tgacgctggt ggacgatacg ctagtgcgta tgaagaaaga gatcggcgag ctggaaaaca
    19981 aattgagcga aacacgcgct cgccagcagg cattgatgtt acgccatcag gcggcaaact
    20041 cgtcgcgcga tgtgcgtcgt cagctggaca gtggcaaact ggatgaagca atggctcgtt
    20101 tcgaatcttt cgaacgtcgt attgaccaga tggaagcgga agcagaaagc cacagcttcg
    20161 gtaaacaaaa atctctggac gatcagtttg ccgaactgaa agccgatgat gcaatcagcg
    20221 agcaactggc acaattaaaa gccaaaatga agcaagacaa tcaataataa catccaggcg
    20281 gcgtccgaac gcgccgccgc tcatcgtcta aggagtcctt atgagcgcgc tatttctggc
    20341 tattccgtta accatttttg tgctgtttgt tttaccgatc tggttatggc tgcattacag
    20401 caatcggtct ggtcgcagtg aattgtcgca aagtgagcag cagcgattag cgcaactggc
    20461 tgatgaagca aaacggatgc gcgaacgtat tcaggcgctg gaatctattc ttgatgcaga
    20521 acatccgaac tggagggatc gctaatggcg ggcattaatc tcaataaaaa attatggcgt
    20581 attccacagc ggggcatggt acgcggcgtc tgcgccggga ttgccaacta ttttgatgta
    20641 ccggtaaaac tggtgcgtat cctggtggtg ctgtcgattt tcttcggtct ggcgctgttt
    20701 accctggttg cttacatcat tttgtcattt gcgcttgatc caatgccgga caacatggcc
    20761 tttggtgagc agctaccttc cagtagcgaa ttgctggatg aagtcgaccg tgaactggcg
    20821 gcaagtgaaa cgcgtttacg cgagatggaa cgttatgtca cttccgatac tttcacgtta
    20881 cgtagccgtt tccgtcaact gtgaggaaag ttatgaatac tcgctggcaa caggccgggc
    20941 aaaaggtgaa gcctggtttc aaattagcag gcaagctggt acttcttacc gcactgcgct
    21001 atggcccggc gggtgtggcg ggctgggcga taaaatcagt tgctcgccga ccgctgaaaa
    21061 tgttgctggc tgtggcgctg gaaccgctgt taagtcgggc tgctaataaa ctggcacagc
    21121 gttataaaag gtgaggggag tttcgcaaaa atggttaaat ctcaggccta taatggatgg
    21181 caattttcat ccatagaagg acgcttacat gtttaaaaaa ggcttacttg ctctgacact
    21241 ggtgttttca ctgcccgttt tcgccgctga acactggatc gatgttcgtg ttccagagca
    21301 gtatcagcaa gagcacgttc agggagccat caatattccc ctgaaggaag taaaagagcg
    21361 cattgccacc gccgttccgg ataaaaacga caccgtgaaa gtgtattgca atgccggacg
    21421 ccagtcaggg caggcaaaag agatccttag cgagatggga tatacccacg ttgagaacgc
    21481 cggtggcctg aaagacatcg caatgccgaa ggtcaaaggt taaaagaatt caccatgagc
    21541 ggcgcttatg cgccgttttt ttctgtcaca cattatttac atccccatag attatttgcg
    21601 tcagctcaca aatacgcttt ttcccaggta aaaaaagatt tcctgcgtga ctaaacccct
    21661 tgagctcaat tgacagttta ttttatgcgg agtagtctct cgcttcatgg gaccgctacc
    21721 acggaaaggc aacatgaaac agaaaattac ggattacctg gacgaaatct acggtggaac
    21781 atttaccgcg actcatttac agaaacttgt aacgcgtctt gagagtgcga aacgattaat
    21841 tacacagcga cgtaaaaaac actgggatga aagtgatgtc gtgttaatta cctatgccga
    21901 tcaatttcac agtaatgatt taaagccact acccacattt aatcagtttt accctcaatg
    21961 gctgcaaaga attttttcac atgttcattt attacctttt tatccatggt catctgatga
    22021 tggattttcg gtaattgatt atcatcaggt cgccagtgaa gcgggggagt ggcaggatat
    22081 tcagcaactc ggtgaatgca gccatttaat gtttgatttt gtctgcaacc atatgtcggc
    22141 aaaaagtgaa tggtttaaaa actatttaca acagcagcca ggttttgaag atttttttat
    22201 tgccgttgat ccgaaaaccg atctcagcgc cgtcactcgc ccgcgtgcgt taccgttatt
    22261 aacgccattc cagatgcgcg atcattcaac gcgccattta tggaccacct ttagcgacga
    22321 tcaaattgac ctcaattacc gtagccctga agtgttgctg gcgatggtgg atgtattgct
    22381 gtgctacctg gaaaagggcg cagagtatgt ccgtctggat gccgttggct ttatgtggaa
    22441 agagccggga acaagctgca tccatctgga aaaaacacat ctgattatca aactgttacg
    22501 gtcgattatt gataacgtcg cgccaggcac agtgatcatt accgagacca atgttccgca
    22561 taaagacaac attgcttact ttggcgaagg cgatgacgaa gcgcatatgg tgtaccagtt
    22621 ctcgctgccg ccgctggtgc tgcatgcggt gcaaaaacag aacgttgagg cactttgtgc
    22681 gtgggcgcaa aacctgacac taccttccag caacaccacc tggtttaact tccttgcctc
    22741 tcacgatggc atcgggctta acccactgcg ggggttgttg cccgaaaacg aaatattagc
    22801 gctggtcgag gtgttacagc aggaaggagc attagtaaac tggaaaaata atcccgacgg
    22861 tacgcgtagc ccctatgaaa tgaatgtcac ctatatggat gcgttgagcc gccgtgagtg
    22921 tagcgacgaa gaacggtgtg ccaggtttat tctcgcccat gcgattttgt taagtttccc
    22981 cggtgtgcca gcgatatata ttcaaagcat tctggggtcg cgtaatgatt acgcaggtgt
    23041 cgaaaaactt ggatataatc gtgcgattaa ccgtaaaaaa tactctagta aagagataac
    23101 gacagaactg aacaataaag cgacattaag ttattcggta tattataagt tgtcgcattt
    23161 aattacactt cgtcgcagtc ataaagagtt tcatccggat aataatttca ccattgatgc
    23221 gattaattca tttgtaatgc gaattcaaag aagcaatgcg gatggcgatt gcctgacggg
    23281 attgtttaat gtcagtgaaa atattcagca tataaatatt actgacctgc acggtcggga
    23341 tctgattagt gaagttgata tattgggtaa tgaaataaca ctgcatccct ggcaggttat
    23401 gtggattaaa taaaaaggaa catttcatga ttaaatcaaa aatcgtgctg ttatcagcac
    23461 tggtttcctg caccctgatt tcaggctgta aagaagaaaa taaaaataat gtatccatcg
    23521 aatttatgca ttcttcggtg gagcaggagc gccaggccgt catcagcaaa ttgattgccc
    23581 gttttgaaaa agaaaaccca ggtatcaccg taaaacaagt gcccgtggaa gaagacgcct
    23641 ataacaccaa agtcattacc ctttcacgta gcggttcgct gccggaagtg atcgaaacca
    23701 gccatgacta cgccaaagtg atggacaaag agcagcttat cgatcgccag gcggttgcca
    23761 cggtcatcag caacgtcggt gaaggcgcgt tctacgatgg cgtactgcgg attgtgcgca
    23821 ccgaagatgg cagcgcctgg accggtgttc ctgtcagcgc atggattggc gggatctggt
    23881 atcgcaaaga tgtgctggca aaagctgggc tggaggagcc aaaaaactgg cagcaattgc
    23941 tggacgttgc acagaaactg aatgacccgg cgaataaaaa atacggtatt gcgctgccta
    24001 cagcagaaag cgtgttgacg gaacagtctt tctcccagtt tgcgttatcc aatcaggcta
    24061 acgtctttaa cgccgaaggc aaaatcaccc ttgatacacc agagatgatg caggcgctga
    24121 cctattaccg caaccttgct gccaacacca tgccaggttc taacgacatc atggaagtga
    24181 aagacgcttt tatgaacggc accgcgccga tggcgattta ctccacctat atccttccgg
    24241 ctgtgattaa agaaggcgat ccgaaaaacg ttggtttcgt ggtgccaacc gagaaaaatt
    24301 ctgcggtcta cggcatgttg acctcgctga ccattaccgc cgggcaaaag actgaagaga
    24361 ccgaagcggc tgaaaaattt gtcaccttta tggagcaggc ggacaacatt gccgactggg
    24421 tgatgatgtc accaggcgcg gcgctgccgg tgaacaaagc ggtggtgact accgccacct
    24481 ggaaagacaa cgacgttatt aaggcgctgg gagaattgcc gaatcagcta atcagcgaac
    24541 tgccaaatat tcaggtcttt ggcgcagtag gggataaaaa ctttacccgc atgggcgatg
    24601 tcacggggtc tggtgtggtg agttcaatgg tgcataacgt caccgtgggt aaagccgatc
    24661 tccctggcac gctgcaagcg agccagaaaa agctggatga actgatcgaa ctgcactaaa
    24721 cccaggacag gaatccgcaa tgaacaggct tttttcaggt cgttccgata tgccttttgc
    24781 actgctgctt ctcgcgccca gcttattact gctgggcggt ctggtggcgt ggccgatggt
    24841 gtcgaatatc gaaatcagtt ttttacgtct gccgctcaat cccaacatcg agtcaacctt
    24901 tgttggggtg agtaactatg tgcgcattct ctccgatcct ggtttctggc actcgctgtg
    24961 gatgacggtc tggtataccg cgcttgtggt ggcgggcagc accgtgctcg ggctggccgt
    25021 ggcgatgttt ttcaaccgcg agttccgcct gcgcaaaacc gcgcgttcgc tggtgatcct
    25081 ttcctacgta acgccgtcta tttcgctggt gttcgcctgg aaatacatgt tcaacaacgg
    25141 ctacggcatt gtgaactacc tcggcgtcga tcttctgcat ctctatgagc aggcgccgct
    25201 gtggttcgat aacccgggca gcagctttgt gctggtggtg ttgttcgcta tctggcgcta
    25261 cttcccgtat gcctttatct cgtttctggc gattttgcag accatcgaca aatcgctgta
    25321 cgaagcggca gagatggatg gcgcgaatgc ctggcagcgg tttcgcatcg tcacgctgcc
    25381 cgcgattatg ccggtcctgg cgacggtggt gaccctgcgc actatctgga tgttctacat
    25441 gttcgcggat gtttatctgc tgacgaccaa ggtcgatatt ctcggtgtct atctctacaa
    25501 aaccgccttt gcctttaatg acttagggaa agcggcggcg atctcggtgg tgcttttcat
    25561 cattattttc gctgtcattc tgctgaccag gaaacgggtg aacctcaatg gcaacaaata
    25621 aacgcacact cagtcgaatc ggtttttact gcgggctggc gctgtttctc atcatcacgc
    25681 tgtttccatt ttttgtgatg ctgatgacct cgttcaagag cgcaaaagag gcgatctcgc
    25741 tgcatcctac gctgctgcca cagcagtgga cgctggagca ttacgtcgac atttttaatc
    25801 cggtgatttt cccgtttgtc gactacttcc gtaacagcat ggtggtgtcg gtggtttcat
    25861 ccgtggtggc ggtatttctc ggcattctcg gggcttacgc gctttcccgc ctgcgcttta
    25921 aagggcggat gaccatcaac gccagctttt acacggtgta catgttctcc ggcattttgc
    25981 tggtggtgcc gctgttcaaa atcatcaccg cacttggcat ttacgacacc gagatggcgc
    26041 tgatcattac catggtgacg caaaccctgc ccaccgccgt gtttatgctg aaaagctact
    26101 tcgacaccat ccctgatgag atcgaagaag cggcgatgat ggatggcctc aaccgtctgc
    26161 aaatcatctt ccgtatcacc gtgcccctgg cgatgtccgg gctgatatcg gtgtttgtct
    26221 actgctttat ggtggcgtgg aacgactatc tgtttgcgtc gattttcctc tccagcgcca
    26281 gcaatttcac cttaccagtg ggcctgaacg cgctgttcag tacgcctgat tacatctggg
    26341 gacggatgat ggcggcctcg ctggtgaccg cattgctagt ggtgattatg tatgcgcttt
    26401 cggaacattt tattaagagt ggtttgaccg ccggtggcgt gaagggctaa agcggccagt
    26461 ttctttacaa ggagttttac atgaaaaagt tagtagccac agcaccgcgt gttgctgcgc
    26521 tggttgagta tgaagagcgg gcgattttag ccaatgaagt gaagatccgc gtgcgtttcg
    26581 gcgcgccgaa acacgggacg gaagtggttg atttccgcgc cgccagcccg tttattaatg
    26641 acgactttaa tggcgaatgg cagatgttca ccccgcgtcc ggcagatgcg ccgcgcggca
    26701 ttgagtttgg caagttccag cttggcaaca tggtggttgg cgacattatc gagtgcggca
    26761 gcgacgttaa cgactacgcg gtgggcgaca gcgtatgcgg ctacggcccg ctctccgaga
    26821 cggtcatcat taacgcagtg aataactaca agctgcgcaa aatgccgcaa ggcagctcct
    26881 ggaaaaacgc cgtctgctac gacccggcgc agtttgccat gagcggcgtg cgcgatgcca
    26941 acgtacgcgt aggggatttt gtggtggtgg tagggcttgg cgcgatcggt caaattgcca
    27001 tccaactggc gaaacgcgct ggcgcatcgg tggtaattgg cgtcgatcct atcgcccatc
    27061 gctgtgatat tgcccgtcgc cacggtgcgg atttctgtct taaccccatt ggcgctgacg
    27121 taggtaaaga gatcaaaacg ctgaccggca agcagggtgc cgatgtgatt atcgaaacca
    27181 gtggctatgc cgacgcgctg caatcggcac tccgcggtct ggcctatggc ggcaccatct
    27241 cctatgtcgc gtttgccaaa ccgtttgccg aaggttttaa cctcggacgc gaagcccatt
    27301 tcaataacgc caaaattgtt ttctcccgtg cgtgcagcga gccgaacccg gattatccgc
    27361 gctggagccg taagcgtatt gaagaaacct gctgggaact gctgatgaac ggttatctca
    27421 attgcgaaga tttaatcgat ccggtggtga cctttgccaa cagcccggaa agctacatgc
    27481 agtatgtcga ccagcatccg gaacagagca tcaaaatggg cgtcacgttt taattcaaag
    27541 gaatgaaaag aatgaaaatc ggcacacaga atcaggcgtt ctttccggaa aacattctgg
    27601 agaaatttcg ttatatcaaa gagatgggtt tcgatggttt tgagattgac ggcaaactgc
    27661 tggtcaacaa tctcgaagaa gtcaaagtag caatcaaaga aaccggttta ccggtgacca
    27721 ccgcctgcgg tggctatgac ggttggattg gcgactttat cgaagagcgt cgacttaatg
    27781 gcttaaagca gatcgaacgc attcttgaag cgctggcaga agtgggcggt aaaggtatcg
    27841 tcgttccggc tgcgtggggc atgtttacct tccgcttacc gccgatgacc tcgccgcgca
    27901 gccttgacgg cgatcgtaaa atggtgagtg attccctgcg cgtactggaa caggtcgccg
    27961 cgcggactgg aactgtggtg tatctcgaac cgttaaaccg ctatcaggat cacatgatca
    28021 acaccctcgc cgatgctcgt cgttacatcg tcgaaaacga tcttaaacat gtacagatta
    28081 tcggcgattt ctatcacatg aatatcgaag aagataacct ggcgcaggcg ttgcacgaca
    28141 accgcgactt gctcggtcat gtgcatattg ccgataacca tcgctaccag ccgggcagcg
    28201 gcaccctcga tttccacgcg ctgtttgaac agctgcgcgc cgataactat cagggctatg
    28261 tggtgtatga agggcgtatc cgggcagaaa atcctgccca ggcgtaccgt gattctttgg
    28321 cctggttgcg tacctgctaa gaggtctttg tgaaaagtgc aatgacaagc tctccactgc
    28381 gggtcgcgat aatcggcgca ggccaggtgg cggataaggt tcatgcttcg tactactgca
    28441 cccgcaacga tctggaactg gtggctgtct gtgacagccg cctttcccag gcgcaggcgc
    28501 tggcagaaaa atacgggaat gcatccgtgt gggacgatcc gcaggccatg ctgctggcgg
    28561 tgaaacctga tgtggttagc gtctgctcac ctaaccgttt tcattacgaa cataccctga
    28621 tggcgctgga agcgggctgc catgtgatgt gcgaaaaacc gcccgccatg acggcagaac
    28681 aggcgcggga aatgtgcgat accgcgcgca aacagggcaa ggtgctggcc tacgactttc
    28741 accatcgttt tgcactcgat acgcaacagc tgcgtgaaca ggtgaccaac ggcgttttgg
    28801 gagagattta cgttaccacc gcccgcgccc tgcgtcgttg cggtgttccc ggctggggtg
    28861 tctttactaa taaagaattg cagggcggag gcccgctgat cgacatcggc attcatatgc
    28921 tggatgcggc gatgtatgtg ctgggttttc cagcggtgaa aagcgtgact gcgcatagct
    28981 ttcaaaagat cggcacgcaa aagagctgcg gtcaatttgg cgagtgggac ccggcaacct
    29041 acagcgtcga agattcgctg tttggcacca ttgaatttca taacggcggc attctgtggc
    29101 ttgagacctc atttgcactc aacatccgcg aacagtcgat tatgaacgtc agcttttgtg
    29161 gtgataaagc cggggcgacg ctgtttccag cacatatcta caccgataac aacggtgagt
    29221 taatgacgct gatgcaacgg gaaatggcag acgacaaccg tcatctgcgc agtatggaag
    29281 cctttatcaa ccacgtacaa ggtaagcccg tgatgatagc cgacgccgag cagggataca
    29341 tcatccagca actggtggcg gcgctttatc aatccgcaga aacagggacg cgtgtggaat
    29401 tatgatcagg ccagtaacgt taacggaacc ccatttcagc cagcataccc tgaacaagta
    29461 tgcttcgctg atggcgcagg ggaacggcta tcttgggctt cgcgccagcc atgaagaaga
    29521 ttacacgcgc cagacgcgcg gcatgtatct ggcagggctt tatcatcggg cgggtaaagg
    29581 tgaaatcaac gaactggtga acctgcctga tgtcgtgggg atggagattg ccataaatgg
    29641 tgagattttc tcgttatccc gcgaagcatg gcagcgtgag cttaactttg ccagtggcga
    29701 attacgccgc aacgttgtct ggcgtaccag caacggcgca ggttacacca tcaccagccg
    29761 tcgctttgtt tcggcagacc aactgccgct cattgcgctg gaaatcacta ttacgccact
    29821 ggacgccgac gcgttagtgc tgatttcaac aggcattgac gccacgcaaa ccaaccacgg
    29881 tcgccaacat ctcgacgaaa cccaggtgcg ggtatttggt cagcatctga tgcaggggat
    29941 ctacaccacc caggatgggc gcagtgatgt ggccatcagc tgttgctgta aggtgagcgg
    30001 tgatgtgcag caatgctata ccgccaaaga gcgccgttta ctgcaacata cctgcgcgca
    30061 gcttcacgca ggtgagacgc tgacgctgca aaaacgggtg tggatcgact ggcgggacga
    30121 cagacaagcc gctttagacg aatggggcag tgcgtcgctt cgtcagcttg aaatgtgcgc
    30181 gcagcagagt tacgaccaac ttcttgcagc atcaacagaa aactggcgtc aatggtggca
    30241 gaaacgtcgt atcacggtaa acggcggcga tgcgcacgat cagcaagcgt tagattatgc
    30301 gctttatcac ctgcgcatca tgacgcctgc ccacgatgag cgcagcagca ttgcggcgaa
    30361 aggtttaacc ggcgaaggct acaaaggcca cgttttctgg gatacagaag tatttttgct
    30421 accgttccat ctgtttagcg atccgacggt tgcccgaagt ttactgcgtt atcgctggca
    30481 caacttgcct ggcgcgcagg agaaagcgcg acgcaacggc tggcagggcg ctctgtttcc
    30541 gtgggaaagc gcgcgtagtg gcgaagaaga gacgccggaa tttgccgcca ttaacattcg
    30601 taccgggttg cggcaaaaag tggcctctgc gcaggcggaa catcatctgg tggccgatat
    30661 cgcctgggcg gttattcaat actggcggac cacgggggat gaaagtttca ttgctcatga
    30721 aggcatggcg ctacttctgg agacggcaaa gttctggatt agccgcgcgg tgagggttaa
    30781 cgaccgtctg gaaattcatg atgttattgg gccagatgaa tataccgaac atgtcaataa
    30841 taacgccttc accagctata tggcgtatta caacgtccag caggcgctga atatcgctcg
    30901 tcagtttggc tgtagcgacg acgcgtttat tcatcgtgca gaaatgtacc tcaaagaact
    30961 gctgctgcca gaaattcagc ccgacggcgt tttgccgcag gatgattcgt ttatggctaa
    31021 gccggtgatt aatctggcga aatacaaagc ggcggcgggg aagcaaacca ttctgctgga
    31081 ttattcacgc gcagaagtga acgagatgca gatcctcaaa caagctgatg tggtgatgct
    31141 caattacatg ctgccggagc agttctcagc ggcatcgtgt cttgccaatc tgcaatttta
    31201 tgaaccgcgc actattcacg actcgtcatt aagtaaagca atccacggca ttgttgccgc
    31261 acgctgtggc ctgctgaccc aaagttatca gttctggcgc gaggggactg aaatcgatct
    31321 tggtgctgat ccacatagct gtgatgacgg tatccacgct gccgcaactg gcgctatctg
    31381 gctgggggcg attcagggtt ttgccggggt gagcgtgcgt gacggtgaat tacatctcaa
    31441 tccggcgtta cctgagcagt ggcaacagtt gtctttccct ctgttctggc agggctgcga
    31501 attacaggtc actctcgacg cgcagcgtat tgcgattcga acttctgcgc ccgtttcact
    31561 gcgcttgaac ggtcagctta tttacgtcgc tgaagaatct gttttctgtt tgggggattt
    31621 tattttgccc ttcaatggga ccgctaccac gtatcaggag ggtgaatgaa actgcaaggg
    31681 gtaattttcg atctggatgg agtaatcaca gatagcgcac atctgcattt ccaggcgtgg
    31741 cagcagattg ccgctgaaat tggcatcagc attgatgcgc agtttaacga attcctaaaa
    31801 gggatcagcc gcgatgagtc cctgcggcga attctgcagc acgggggtaa agagggcgat
    31861 tttaacccac aggaacgggc gcaactggcg tatcgcaaaa atctgcttta cgtccactca
    31921 ctgcgcgaac tgacggttaa tgctgtgctg cctggcattc acaatttgct ggtagatctc
    31981 cgagcacagc agattccggt tgggctggct tctgtctccc tgaatgcgcc gacgatttta
    32041 gcagcgctgg agctgcgcga gtttttcacc ttctgcgcgg acgcttccca actcaaaaat
    32101 tcgaaaccgg acccggaaat ctttctcgcc gcctgtgcag ggctgggcgt gccgccacag
    32161 gcatgtatcg gcattgaaga tgcgcaggcg ggcattgaag ccattaacgc cagcggtatg
    32221 cgctcggtgg ggatcggcgc gggcttaacc ggggcgcaat tattgttgcc ctcaacggac
    32281 tccctgacct ggccgcggtt atcggccttc tggcaaaacg tatagcaaag gaatcaacat
    32341 ggcacagctt tcgttacaac atattcaaaa gatctacgat aaccaggtgc atgtggtgaa
    32401 ggacttcaac ctggagattg ccgataaaga gttcatcgtg tttgtcggtc cgtcgggctg
    32461 cggtaaatcg accaccctgc gtatgattgc cgggcttgag gagatcagcg gcggtgatct
    32521 gttgatcgac ggcaaacgaa tgaatgacgt tccagccaaa gcacgcaata tcgcgatggt
    32581 gttccagaac tacgcgctgt atccgcatat gacggtctac gacaacatgg cgtttggcct
    32641 gaagatgcaa aaaatcgcca gagaggtgat tgatgagcgg gtgaactggg cggcgcaaat
    32701 tctcggcctg cgtgagtacc tgaaacgcaa gccggacgcg ctttccggcg ggcaacgtca
    32761 gcgagtggcg cttgggcggg cgattgtgcg cgaagcgggc gtgtttttaa tggatgagcc
    32821 gctctctaac ctggatgcca agctgcgcgt gcaaatgcgc gcagagatca gcaagctgca
    32881 tcagaaactg aacaccacca tgatctacgt gacccacgat cagaccgaag cgatgaccat
    32941 ggcgacgcgg attgtcatta tgaaggatgg gattgttcag caggtcggtg cgccgaaaac
    33001 ggtttataac caacctgcga atatgtttgt tgccggattt attggatcac cagcgatgaa
    33061 ttttattcgc ggcacgatcg atggcgataa attcgttacg gaaacgctta aattaaccat
    33121 acccgaagag aaattagcgg ttctgaaaac acaggaaagt ttgcataagc ccatcgtaat
    33181 gggaatacgc ccggaagata ttcatccgga cgcgcaagag gaaaataaca tttccgccaa
    33241 aattagcgtg gcggaattaa ccggtgcgga atttatgctc tacaccacgg ttggggggca
    33301 cgagttagtg gtccgtgctg gtgcgttaaa tgattatcat gcaggagaaa atatcactat
    33361 tcattttgat atgactaaat gtcatttctt tgatgcagaa acggaaatag caatttgcta
    33421 aatacagggg gaaggcattc tcccaggata atacaaggaa caataatgaa aaagttatta
    33481 ccctgtaccg cactggtgat gtgtgcggga atggcctgcg cacaggccga ggaaaagaac
    33541 gactggcact ttaatatcgg tgcgatgtac gaaatagaaa acgtcgaggg ttatggcgaa
    33601 gatatggatg ggctggcgga gccttctgtc tattttaatg ccgccaacgg gccgtggaga
    33661 atttctctgg cctattatca ggaagggccg gtagattata gcgcgggtaa acgtggaacg
    33721 tggtttgatc gcccggagct ggaggtacat tatcagttcc tcgaaagcga tgatttcagt
    33781 ttcggtctga ctggtggttt ccgtaattat ggttatcact acgttgatga accgggtaaa
    33841 gacacggcga atatgcagcg ctggaaaatt gcgccagact gggatgtgaa actgactgac
    33901 gatttacgtt tcaacggttg gctgtcgatg tataaatttg ccaacgatct gaatacgacc
    33961 ggttatgctg atacccgtgt cgaaacggaa acgggtctgc aatatacctt caacgaaacg
    34021 gttgccttgc gagtgaacta ttatctcgag cgcggtttca atatggatga tagccgcaat
    34081 aacggtgagt tttccacgca agaaattcgc gcctatttgc cgctgacgct cggcaaccac
    34141 tcggtgacgc cgtatacgcg cattgggctg gatcgctgga gtaactggga ctggcaggat
    34201 gatattgaac gtgaaggcca tgattttaac cgtgtgggtt tattttacgg ttatgatttc
    34261 cagaatggtc tttccgtttc gctggaatac gcgtttgagt ggcaggatca cgacgaaggc
    34321 gacagagata aattccatta tgcgggtgtt ggggtgaatt actcgttctg ataatgtgct
    34381 aaattgccgg atgcggggcg agcgccaaat ccggcatata tttatttcaa cgacatatgc
    34441 gtagcaacgg taatattttg tggtgacggt tttccggcaa ttagctggaa taataactcg
    34501 cagctttgtt gacctaactc ctgcgtcgga acatcgatgc cgcctggtgc aggcgttaat
    34561 ataaacgaca gcgtttcatt actataaccc accaccgcta actgctgcgg aatagcaatg
    34621 tttttctctg ccgaagcacg ataaatactc attaatttca ggctgtcagt agcaaacact
    34681 gcctcaggca acggtgactg gcttaataat tcccgtgctg cctttaatgc tgtctcatgg
    34741 gtataaccgc cgtcaacaat ccattcatca cgcactgcaa tattatgcgt agccaggctt
    34801 tgcttataac catttacccg atccactgaa acatgaacat caagcggcgt atgcaggcat
    34861 gcaatattct tacacccgct ttcaattaac gcatcggtta gcgcaatact gtcgccataa
    34921 ttatcggtat cgacggaata aacatggctg tattgacctt caactttacc gatcaccacc
    34981 accgggatct cgtatttgtc gagttgtgca aagaaggatt catcggcagg cgaacttagc
    35041 ataataatgc ctttaatcat tttctgctta attttgcttt cgcatttttg cagatcttct
    35101 gctggattgt gcgacgtttg taatatcacg tcgaaacctt cttcttccgc tttggcggtg
    35161 atggcatgca aaacttcaga gaaaaacgga ttacccgccg tagttttggt cgaacgggta
    35221 gaaatcacca taatggcatc aaaacccgaa gaggtcagcg cgcgggccag cttgtttggt
    35281 tgatactgta attcttcaat ggcccgtaac actttttcgc gcgcttccgg ggagatattg
    35341 gtttgcttat tcagcacgcg tgatacggtg gattttgata cgcctgcaac cctggcaata
    35401 tcataaatag taggggacat aggtcaggaa ctccgtccga acgtgaatgc tgcacatctt
    35461 atggagttcg cccgtcgatg acaacacggg tagttgataa tcaatggcct ggcccccaca
    35521 ttcatattct tacgaacgat tttttttctg gtcttcagag cagcacagga cagcaatgaa
    35581 gcgacttaaa aatgaactca atgcgctggt gaatcggggt gtcgacagac atctgcgcct
    35641 cgctgtaacc ggacttagcc gcagcggcaa aacagcgttt atcaccgcga tggttaatca
    35701 gttgctcaat attcatgccg gagcacgttt gccgctttta agtgcggtgc gtgaagagcg
    35761 cctgctgggc gtaaaacgca ttccccagcg tgactttggc attccgcgct tcacctatga
    35821 tgaagggttg gcgcagttat atggcgatcc tcccgcctgg ccgacgccta cgcgcggcgt
    35881 cagtgaaatt cgcctggcgt tacgttttaa atcgaacgat tcgctgctgc gccactttaa
    35941 ggacacctcc acgctgtatc ttgagattgt ggattatccc ggcgaatggc tgctcgacct
    36001 gccgatgctg gcacaggact attttagctg gtcgcgccag atgacgggct tactcaatgg
    36061 tcagcgcggc gaatggtcgg cgaaatggcg aatgatgtgt gaaggtctgg acccgttagc
    36121 gcctgccgac gaaaatcgcc tggcggatat tgccgctgca tggaccgatt atctccacca
    36181 ctgcaaacag caggggctgc actttattca gccaggacgc tttgttttgc ctggcgatat
    36241 ggcaggagcg cccgcgctgc aattcttccc atggccagat gtcgatgcct ggggcgagtc
    36301 caaactggcg caggccgata aacacaccaa cgccggaatg ttgcgcgagc gttttaatta
    36361 ttactgcgag aaagtggtga aggggttcta taagaatcac ttcctgcgtt ttgaccgcca
    36421 gattgtgctg gtggactgcc tgcaaccgct caacagtgga ccacaggcat ttaatgatat
    36481 gcgtctggcg ctgacgcagc tgatgcaaag tttccactac gggcagcgta ccctgttccg
    36541 gcgtttgttt tcgccggtta tcgataagct attgtttgct gccactaaag cggaccatgt
    36601 gaccatcgat cagcacgcta atatggtttc attgctgcaa caactgattc aggatgcctg
    36661 gcaaaatgcg gcgttcgaag ggatcagtat ggactgtctg gggctggcgt ctgttcaggc
    36721 gaccaccagc ggaattattg atgttaacgg tgagaaaatc ccggcgctgc gcggtaatcg
    36781 actcagcgat ggcgcaccgc tcactgttta tcctggcgaa gttcccgcac gtttgccagg
    36841 tcaggcattc tgggataagc aaggcttcca gtttgaggcg tttcgtccgc aggtgatgga
    36901 tgtcgacaaa ccgctaccgc atattcgtct tgatgctgcg ctggaatttt taataggaga
    36961 taaattgcga tgaccgaacc gttaaaacca cgtattgatt tcgacggtcc actggaggtc
    37021 gatcagaatc caaaattcag ggcgcagcag acctttgacg aaaatcaggc gcaaaatttt
    37081 gccccagcca cgctcgatga agcgcaggaa gaagaggggc aagttgaagc ggtgatggac
    37141 gcagcgttac gtccgaaacg cagcctgtgg cgcaaaatgg tgatgggcgg gctggctctg
    37201 tttggcgcaa gcgttgtcgg gcagggcgtg cagtggacaa tgaacgcctg gcaaacccag
    37261 gactgggtgg cgctgggcgg atgtgccgct ggggcattga ttatcggcgc tggcgtaggt
    37321 tctgtggtaa cagagtggcg gcgcttatgg cgcttgcgac agcgcgccca tgaacgcgac
    37381 gaagcgcgcg atttgttgca cagtcacggc acgggtaaag gccgtgcatt ctgcgaaaaa
    37441 ctggcgcagc aggcgggcat cgatcagtca catccggcgc tgcagcgctg gtatgcctca
    37501 atccatgaaa cgcagaacga tcgtgaagtg gtcagtttgt atgctcacct ggtccagcca
    37561 gttttagatg ctcaggcgcg gcgcgaaatc agccgctcag cggcggaatc aacgttaatg
    37621 attgccgtta gcccactggc gctggtggat atggcgttta tcgcctggcg caatctgcgt
    37681 ttgattaatc gcatcgccac gctgtatggc attgaactgg gatattacag ccgtttgcgc
    37741 ctgtttaagc tggtattgct gaatattgct tttgccggag ccagcgaact ggtgcgcgaa
    37801 gtggggatgg actggatgtc gcaagatctc gctgcgcgtt tgtctacccg ggcagctcag
    37861 gggattggtg ctggacttct gacggcacga ctggggatta aagctatgga gctttgccgc
    37921 ccgctgccgt ggattgacga tgacaagcca cgcctcgggg atttccgtcg tcagcttatc
    37981 ggtcaggtga aagagacgct gcaaaaaggc aaaacgccca gcgaaaaata atgcaatatc
    38041 gggtgctgac cggatatctt tacgccgaag tgcccgtttt tccgtctttg tgtcaatgat
    38101 tgttgacaga aaccttcctg ctatccaaat agtgtcatat catcatatta attgttcttt
    38161 tttcaggtga aggttcccat gcgtctggaa gtcttttgtg aagaccgact cggtctgacc
    38221 cgcgaattac tcgatctact cgtgctaaga ggcattgatt tacgcggtat tgagattgac
    38281 cccattgggc gaatctacct caattttgct gaactggagt ttgagagttt cagcactctg
    38341 atggccgaaa tacgccgtat tgcaggtgtt accgatgtgc gtactgtccc gtggatgcct
    38401 tccgaacgtg agcatctggc gttgagcgcg ttactggagg cgttgcctga accagtgctc
    38461 tctgtcgata tgaaaagcaa agtggatatg gcgaacccgg cgagctgtca gctttttggg
    38521 caaaaattgg atcgcctgcg caaccatacc gccgcgcaat tgattaacgg ctttaatttt
    38581 ttacgctggc tggaaagcga accgcaagat tcgcataacg agcatgtcgt tattaatggg
    38641 cagaatttcc tgatggagat tacgcctgtt tatcttcagg atgaaaatga tcaacacgtc
    38701 ctgaccggtg cggtggtgat gctgcgatca acgattcgta tgggccgtca gttgcaaaat
    38761 gtcgccgccc aggatgtcag cgccttcagt caaattgtcg ctgtcagccc gaaaatgaag
    38821 catgttgtcg aacaggcgca gaaactggcg atgctaagcg cgccactgct gattacgggt
    38881 gataccggca ccggaaaaga tctctttgcg tatgcctgtc atcaggcaag ccccagagcg
    38941 agcaaacctt acctggcact gaactgtgcg tctataccgg aagatgcggt cgagagtgaa
    39001 ctgtttggtc atgctccgga agggaagaaa gggttctttg agcaggcgaa cggtggttcg
    39061 gtgctgttgg atgaaatagg tgagatgtca ccacggatgc aggcgaaatt actgcgtttc
    39121 cttaatgatg gcaccttccg tcgggttggc gaagaccatg aggtgcatgt cgatgtgcgg
    39181 gtgatttgtg ctacgcagaa gaatctggtc gaactggtgc aaaaaggcgt gttccgtgaa
    39241 gatctctatt atcgtctgaa cgtgttgacg ctcaatctgc cgccgctacg tgactgtccg
    39301 caggacatca tgccgttaac cgagctgttc gtcgcccgct ttgccgacga gcagggcgtg
    39361 ccgcgtccga aactggccgc tgatctgaat actgtactta cgcgttatgc gtggccggga
    39421 aatgtgcggc agttaaagaa cgctatctat cgcgcactga cacaactgga cggttatgag
    39481 ctgcgtccac aggatatttt gttgccggat tatgacgccg caacggtagc cgtgggcgaa
    39541 gatgcgatgg aaggttcgct ggacgaaatc accagccgtt ttgaacgctc ggtattaacc
    39601 cagctttatc gcaattatcc cagcacgcgc aaactggcaa aacgtctcgg cgtttcacat
    39661 accgcgattg ccaataagtt gcgggaatat ggtctgagtc agaagaagaa cgaagagtaa
    39721 gcgtgaatat gcctgatggt gcaacaccat caggcatatt aacttatgct ttcagtacag
    39781 ccagagctgc ttcgtaatcc ggctcggtgg tgatttcatc caccagctgg ctgaaaatca
    39841 cattgtcatt ttcatcaata accacaacgg cacgcgctgc cagacctttc agtgggccat
    39901 cagcaattgc cacaccgtag gcttgcagga attcagcgtt acggaaagtg gagagagtga
    39961 taacgttgtt cagaccttct gcgccgcaga aacgagactg ggcgaacggc agatcggcag
    40021 agatacacag cacaacggtg ttgtcgatct cggttgccag ttggttaaac ttacgtactg
    40081 atgcggcgca aacaccggta tcaatactcg ggaaaatgtt cagcactttg cgtttacccg
    40141 caaactgacc gagggtgacg tcagacagat cttttgccac gagagtaaaa gtctgcgctt
    40201 tgctacccgc ctgcgggatg gaattggcga ctgtaaccgg gttgccctgg aaatgaacgg
    40261 tttgtgacat gattatcttt cctgtttaca tatagttaac gtcacaccta gtttatgcta
    40321 actgtcaata acacagcaaa cgctatttgc gcttaatccg cagaccaccg cgacaacaag
    40381 gagtaaagat gagaaccgtt aaggtatttg aggaggcctg gcccttacat accccgtttg
    40441 tgattgctcg gggaagtcgc agtgaagcgc gcgtggtggt ggttgaactg gaagaagagg
    40501 gtattaaagg caccggcgaa tgcacgccgt atccgcgtta tggggaaagt gatgcctcgg
    40561 taatggcgca aattatgagc gtcgtgccgc aattagagaa agggctgaca cgggaggagt
    40621 tgcaaaaaat cctccctgcc ggcgcagcac gtaatgcgct ggattgtgca ttgtgggatc
    40681 tggcggcgcg aaaacagcag caatcgctgg ctgatttgat cggcataacg cttcccgaga
    40741 cagtcaccac ggcacagacg atagtcatcg gtacgcctga tcagatggcc aatagtgcat
    40801 caacactctg gcaggcaggc gcgaaattac tgaaagtgaa gctggataac catcttatca
    40861 gtgagcggat ggtggcaatt cgcacagctg tgcccgatgc gacgctgatc gttgatgcaa
    40921 atgaatcctg gcgtgcagaa gggttggcgg cgcgttgcca gctattggca gatttaggcg
    40981 ttgcgatgct tgaacaaccg cttcctgcgc aggacgatgc ggcgctggag aattttattc
    41041 atccgttacc gatttgtgct gatgaaagtt gtcatactcg tagcaatctg aaggcgctga
    41101 aagggcgcta tgagatggtt aacattaagc ttgataaaac cggtggtctg acggaagcgc
    41161 tggcgctggc gactgaagcg cgtgcacaag gttttcgtct gatgctgggc tgcatgttgt
    41221 gtacctctcg tgccattagc gccgctttac cgctggtgcc gcaggtcagt ttcgccgatc
    41281 ttgacggacc gacctggctg gcggtagatg tggaaccggc gcttcagttc acgacgggcg
    41341 aattgcatct ttaggatgcc agcgcagcaa gtttgccatc gcaaagagat atttttcgct
    41401 ggcttcgtcg gaggagatgg gtggaaactc tgcggtgatg caatgcaagt tgagatctgc
    41461 acaccaactg ccgaaagagc caggcgtttc ataaccgacg ctggttacca gaggcaattc
    41521 aaacgcctgg gccagccatt cacctaattc gctgtgtctg ggatcttcaa tacaggccag
    41581 cggatcgtgg aatgagacca cccaggcagg ttgaatgcgg tgtataagct ggcacagcgc
    41641 ctgggtttca ggttcggagc caggtttgtc acctgtcagt aaaacgacat cacgttcttc
    41701 agcggcgctg ttccagcgat aaactgtttc accttccttc cagttcgctg ccggaaagtt
    41761 tcggtttaaa tccacacaat tagcattggc ccgtaaccct aactggcagc cgtcaggatt
    41821 cacacacagc accacatgat ggcggcgcaa agaaggtgtc aaagtccgca gagcacagga
    41881 gagcgtgacg atcgaagagt tttcatcacc gtgagtgcca gccagaatca aaccactttc
    41941 gtgactggct gcaggggccg gaaaccagat taacggcgcg cccaataatg aacgtccgta
    42001 atgttctgtt ccgggcggaa atgcgccgcg ttcggcgcgt gggcgagtta cggtcatgat
    42061 catctctggt tcaggaagta ttacaggcag tgttgtgcaa attttgcgga ttatcaaatc
    42121 cttttcttca acgctatgtc atcgatcttg tatcactttt ttgccggaag ttgtttgtat
    42181 tttcattgat gtgcagatat tagaattgtc gtaatgttat atacatggcg tattttattc
    42241 ttactggttc tacacatgtt aatcgcatct gattgatcag ttcaccttaa taagtttaat
    42301 aaatcgtact gcacatagta cagtacgatg gtagctatat aattgattta catgaagaag
    42361 ggcttatcac catcagtccc gggttgattg atgcctatat tctcaccaat gtgtttctca
    42421 ggtttatcga tgatatctgt gattaatgct gcgacactct tacgtgatac gatcgtacct
    42481 ttgaatggtt cattgcgtga ggtaagctca taatcaataa tatcttcatc agtcagccag
    42541 gcagggcgaa gaattgtata ttccagtcca gaagcctcaa ttgcatccgc agccctgcgg
    42601 aatggtttta ggggttcacc aataactgca ttattccatt cgccaaattt tcctggaact
    42661 tcatcataaa taccgaggga aagaacaaag atcagacgtt taacatcaca ggctttcatc
    42721 gcagcgatca ctgaatttgc ctgaatatct aaatcttccc ccgtaaggtt ggcatacaca
    42781 acatcctgtc cctgcatggc ctgcttcagt gctgcatgat tcagtacatc ccccataata
    42841 atcttgctat tagttggata gggtttgtgg atttttgctg gctgacgggc aaataaagtt
    42901 tgtttaatgg tttgtttatc tgccagttga ttaatcacat ggcgggctat ctgaccgcca
    42961 gcaccaagaa tcagtatatt tttcatgaaa cttttcccag attattggca gcaatatgcc
    43021 aggccatatc actattacgg atttgaatat gtttcattac agagtttcct caaagaacgg
    43081 agctaatact gaaattgctt cagccacata gactttgccg tcataaagat ccatatggtt
    43141 tgcgccttca acaatgtgat agcgtttatc cttgcttgat gctcgatcgt acaggtcgtc
    43201 actcatccat ttgctcccag cctggctgcc cgccacaatc tgtatcggct gagtcaggta
    43261 cacttccgcc atatggtaag catcataggt aataatctgg ttaaggctgc gcaaagtagc
    43321 gtaacccggt gctgttggat actgcgcgcg aggggtgtgg taatactccc aggcctgacg
    43381 cagttcttcg ttcggcgcat cggactcctt cattggtgcc agtggcatag tggcgtattc
    43441 tccgctgcta atatcgctgg ttcgggcgtc tgaacccgcg tcaacgtatg gtagggcatc
    43501 aatagatttc acattgtttt cccaaccatt acggaacatc gaaccaatat tgaccgcact
    43561 aacagtaccg atggccttga tgcggcgatc ctgaattgca gcattggctg tatatcctgc
    43621 tccggcacaa attcccatcg caccaattcg ggtattgtcg acataagaaa gcgttgtcag
    43681 gtaatcaatc acggcactga cgtcttcagt acgaatgtat gggttttcta actgacgcgg
    43741 ctcgccgccg ctttcacctt gataagatgc gtcataagca atagtgacaa atcccttttc
    43801 cgccagtttt tcggcatagg ttccggccgt ttgctcttta acgcccccac ctgggtgaga
    43861 taccacaatt gccggatact gacgggtttc atcaaatttt ggagggaaat agatcactgc
    43921 agacaaagag atggttggat tattgctgtt agtgaagctg actttattat tcatcattcc
    43981 gttcctcatg gagttgtcgg ttcgttttaa cggttggtga tatcactata gatattgatc
    44041 attaagttga ttagacccaa aatcatgatt agacctatca tttaaatgat taatagattg
    44101 tatggagata ctgaatgaag cgtgaagaaa tcgctgatct gatggcgttt gtcgttgttg
    44161 cagaggagcg tagcttcact cgcgcagcag cccgcctgag catggcgcag tcagctttaa
    44221 gccagatagt gcgccgtata gaagaacgat tgggattgcg gcttctgacg cgaaccacgc
    44281 gcagcgttgt acccactgaa gcgggcgagc atcttttgtc tgttcttggc cctatgttgc
    44341 atgacataga ttctgccctg acatccctga gcgatctgca gaaccgccca tccgggacaa
    44401 tacgtattac tactgtagaa catgcagcaa aaacgatatt gttaccagct atgcgcacat
    44461 tcctgaaatc gcatcctgaa attgatattc agcttaccat tgattatggt ttgaccgatg
    44521 tcgtttctga acgttttgat gcaggcgtcc gtctgggtgg ggagatggat aaagatatga
    44581 tcgccattcg aatcgggcca gacataccaa tggctattgt tggctcaccg gattattttt
    44641 ctcgtcgaag tgctccaacg tcagtgtcac aattaataga tcatcaggca attaatttgt
    44701 atcttcccac atcgggtaca gcaaatcgct ggagattaat acgcggtgga cgtgaagttc
    44761 gtgttcgcat ggaaggtcag cttttactga atacgataga cctgatcatt gatgctgcaa
    44821 ttgatgggca tggattggcg tatctacctt atgatcaggt tgagcgggct attaaagaaa
    44881 aaaaactgat acgtgttttg gataaattca caccagattt acccggttat cacctgtact
    44941 atccacaccg tcgacatgct ggctcggcat tctcattatt tatagatagg ctgaagtata
    45001 aaggtgctgt ttagcactac ttgctgatac attaatttaa ttcttctctt aacgtattct
    45061 cagttccttt caacgttttg gttatttttt attcttcgca caatggcgac agatgctgat
    45121 tatgataccg aaaacgggtt tgaacgtgcg aagcccgaac gtagtgttcg gagttctatg
    45181 tgctttaccg cattttggag actattattt acactaaatc tgatttgata tattgatact
    45241 taaaacattt gatgcttcct ttgtcacttt tttgatggaa gttgtttgca tttctttaag
    45301 gcgaaacaaa taattacgca tcaattttaa tgtcggttag agggaaactt atgaagcact
    45361 ctgtttcagt cacgtgttgt gtgctgttgg tcagcagcat ttctctttcg tatgctgctg
    45421 aagttccgag cggcacagta ctggcagaga agcaggagct ggtgcgccac attaaagatg
    45481 agccagcgtc gctggatccc gctaaagccg tgggcctgcc ggagattcag gtcattcgcg
    45541 atctgtttga aggactggtg aatcagaacg agaaagggga gattgtcccc ggcgttgcga
    45601 ctcagtggaa aagtaatgac agccgtatct ggacttttac cctgcgcgat aacgcaaaat
    45661 gggcggatgg cacaccggta acggcgcaag attttgtcta cagctggcaa cgtctggtgg
    45721 acccgaaaac gctatcgcca tttgcatggt ttgccgcgct ggcgggaatc aacaacgcac
    45781 aggcgattat tgatggtaaa gccacgcctg accagcttgg cgtcaccgca gttgatgccc
    45841 atactttgaa aatccagctt gataaaccat tgccgtggtt tgtgaattta accgctaact
    45901 ttgccttctt cccggtgcaa aaagccaacg tagaaagcag taaagagtgg acgaaacccg
    45961 gaaatctgat cggcaatggc gcttatgttc ttaaagagcg cgtagtcaat gaaaaactgg
    46021 tcgtagtgcc gaatacccat tattgggata acgccaaaac ggtactgcaa aaagtgacct
    46081 tcctgccaat taatcaggaa tccgcagcca ctaagcgtta tctcgcgggg gatattgata
    46141 tcaccgaatc cttcccgaaa aatatgtatc agaagttgtt gaaggatatt ccggggcagg
    46201 tttatacgcc gccgcagctc gggacctatt attatgcgtt taacacgcaa aaagggccga
    46261 cggcagatca gcgcgttcgc ctggcattaa gtatgacgat agatcgccgc ctgatgaccg
    46321 aaaaagtgtt ggggacgggc gaaaagccag cgtggcattt tacaccagat gttaccgcgg
    46381 gatttacgcc ggaaccttcg ccgtttgaac aaatgagtca ggaagaactg aatgcgcagg
    46441 caaaaacttt gttgagcgca gctggttatg gtccgcaaaa accgctgaag ttgacgcttt
    46501 tgtataacac ttcagaaaac catcaaaaaa ttgcgattgc tgtagcatcg atgtggaaaa
    46561 agaacctggg cgtagatgtt aaattgcaaa accaggaatg gaaaacctat atcgatagcc
    46621 gtaataccgg caattttgat gttatccgcg cctcgtgggt aggggattat aatgacccct
    46681 ccactttcct gacattatta acgtcaacgc attccgggaa tatttcacgc tttaacaatc
    46741 cggcatatga caaagttctg gcccaggcat cgacggaaaa taccgttaaa gcgcgtaatg
    46801 ccgattacaa cgcggcagaa aaaatcctca tggagcaagc accgattgca ccaatttatc
    46861 aatataccaa tggacgatta atcaagccgt ggctgaaagg ttatcccatt aataaccctg
    46921 aagatgtggc gtacagtcgg actatgtata ttgtgaagca ttgatgtgat gggaactggc
    46981 gttacccttg tggataacgc cagtgatgtc tgatttagcg accctggtcc ggcggtgtaa
    47041 tgttatccat atacagcgtc tggctgggga aggcaaagtc cgcgccgtgt gactgtacaa
    47101 tatcgataat cttcaaataa acgtcttgct gtgcagcaag ccactcagcc cataccgtag
    47161 ttttggtaaa gcaataaacc ataatattta atgacgaatc agcaaactgg ttgaaataaa
    47221 ccagtaaggt ttgtcgctgg tcgatggccg ggtgattttt cagcatctca cgtacggctt
    47281 cgacaataac gcccactttt gccgcatcct cataacgtaa accaatggtc gtggtaatgc
    47341 gccggttggt cattcgtcca ggattttcta cgctgatcga cgaaaacagc gagttcggta
    47401 cgtacaatgg acgattatca aaggtcttaa ttttggtaat tcgccagcca atttccgtta
    47461 ctgtaccttc gatatttctg tccggtgaac ggatccagtc gccaatactg aaaggacggt
    47521 cgaaatagag cataatcccg gaaaagaagt tactcagaat atctttaccg gccataccga
    47581 cagccagacc accaatacca ccaaaggtca acaagccaga aaggctcatg ccgaaatgtt
    47641 cgccataaag cagaacaagc accacaataa tggtgatttt gataatgcgc gacatgatcc
    47701 gcgcactggt gatatcacga ccttttttaa tctgctgttt ttcaaactga ttaatcagca
    47761 gaaatagctt aatcgtcaga ataaccgcaa tcagggacgt acagataaaa tcgataacgc
    47821 ctggggtgat aaatttgagt ttatagtttt ctataacata gttaatgatg ctaccaacag
    47881 cactaataat tatggtgtag attaaaaatt gcaccgcatg gaataaaaat ccttttcttt
    47941 tacgatctcc acggcgaaac caaaagctca tcagaatcaa tgctgcgcag ctaccgaaaa
    48001 taatgaccag attaagcgca ttatttgtaa acagttcagc gatcattgtt ttatcaggct
    48061 cctccagata attgtcgtca tgccggaaac ccctggcggg actattttac cgcgacaatt
    48121 cattcagatc atcaatagtc agggaaggaa gtagcaacat tagctaacta atcaatgcgt
    48181 tgattgtaaa tccagctaag aggtgagatt ttcagagcag acaacggtga aatgtcatgg
    48241 tattgttatg tttaggtaac aagaaatttg tctgcacaag gattacatca tgattatggc
    48301 gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg ttttcattat
    48361 tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt acaatattcc
    48421 gctgtctgag tggacgacat caatgtatgt gattcagtca tcgatgattt ttgtttatag
    48481 cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg gcggcgaaga
    48541 gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa gagtcgttaa
    48601 tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt tatgaccagc
    48661 aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccaaggaa tgctgctgaa
    48721 ataccggtgc gtccgaccgt gcccagaacc acaatccccg cctgtaagtg ctccgccaaa
    48781 tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc attaatgccg
    48841 aatttctgcc gcagagcttt cattgccagc aaatgttgcc cacgaatggc atcgttataa
    48901 acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac cggataagcg
    48961 ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt ctctttgacc
    49021 agtttttcat tgagcgcatt atggtacggc tcttcactgg cgagattcac cgccaccagc
    49081 gccttacccc cttccggcca cggctggtct ttcaccatcc acaccgggct tgggcatttg
    49141 cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc atgttggtgc
    49201 gccattttta gcaccaaatc gtgcccgccg ctgatcactt cctgaatgat ggcttcgaaa
    49261 ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag ataatatttt
    49321 gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat agcggtacgt
    49381 tcgtccggag agagcagggt ggtcatttcg tatgagaagt catagatcgg caaaaaggct
    49441 ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc tggttggtcg
    49501 tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca gggtctcctt
    49561 acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt gaatgaaaca
    49621 gtgatgaacc ttctgtcaga tcaataaatc agaaaaattt aatggtatga cagaaggata
    49681 gtgagttatg cggaagaatc aggcaacgtt acgcgtatga ccagcaagct gggccagcgc
    49741 atcgttattt tcgatagtga tgtatttacc tttgactgcc agcataccgc ttttctggaa
    49801 gcgacccagc agacggctga tggtttcaac cgtcaggccc agatagttac cgatatcacc
    49861 acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa aacgacggga
    49921 caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca acagcaggat
    49981 catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct gacgcagatt
    50041 cggcatttta ccagacaaat cgtccagcgt ttcgaacggg atttcacata ccatcgaggt
    50101 ttccagcgcc tgcgcgaaac tcgggtgatg accgctgccg atggcatcaa atcccaccag
    50161 atcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga tggtataact
    50221 tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatctccag ccttaaacag
    50281 cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct gatcaagctc
    50341 atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct ggcaatggat
    50401 agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca taggtctgct
    50461 caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt aagtctaagc
    50521 aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc tatttgaaaa
    50581 cccttaagtt gttaagggtg actttacata aaagtgtgaa caagctggca caaattgttt
    50641 aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg ctgaactccg
    50701 cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg tacgacgatg
    50761 ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg gcggcctaat
    50821 tgttcagcca gttggccata atgcattacc tgcccgcagg ggatagtgcg cagtgttttc
    50881 cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc aataatgcta
    50941 agattaccgg cgaaatattc acgaagcttg tcgcttaaac cacctgggtt ggtggcagaa
    51001 atgcgctcat agctttcttt gcgataatgg atgtccagca gctgcaccat gcgttcgctg
    51061 tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac ccacagtgga
    51121 cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct cttaagacaa
    51181 acgtgggtaa atacccggac ctatcggcaa gccaacaaga taccacgcca gcaacatcag
    51241 cagccatacc accaaaaaga taagtggata gggtaagact aacgaatagt aagtacccag
    51301 tttcgcgtct ggtttgtagc gttgcaggaa tccaagaaac agtggaacaa aaggagatac
    51361 cggcgctaaa ggcaatacgg atgagtcggc aatacgaaag agaatttgcg caaatgccgg
    51421 gtgaaagcca agtagcataa acattggtac gaaaatgggg gccagaatcg accaaattgc
    51481 ggaaccgctg gcgataaaca tgcataagaa agaggaaagc aacgccagac cgacaaacgc
    51541 cgggatgccg ctaagccctg aactttccag tatatcggtc agccccacgg cgatgaattt
    51601 ccccatgttg ctccagttaa acatggcgac aaactgggcg aggggaaaaa ccatcacgat
    51661 aaatcccgcc atctctttca tcggttcaat cattaaatgc ggtaaatccg cctgatgtcg
    51721 aattgtgcgg gtagcgatgc cataagccag cgagaccaca aagaaaaaaa gaatgatcag
    51781 tggcacgata cctttaataa agggtgatgg catcacggtg tgattaatcg gatcgcgcaa
    51841 tatcccgttt tccgggatca ccatcagcgc aatcgaagca ataaaaagta gcgatacgac
    51901 acctgctatg cgtaaaccaa aatgctgact ttcggtcaat gtctgcagtt tctcatcgct
    51961 gtttccctgc cattgaccta accgtggctc gatgattttg tcggttatca ggccgccaac
    52021 aatcgtcagt acgactacgg agctggccat aaaataccag ttatcaatta cactgacgtg
    52081 catttgcgga ttgaacgcag ccgcagcttc tgtgctgatc cccgacagca acacgtcggt
    52141 tgtgacaatc agtaaattag ccgtaaagcc gcaacctacg cctgcaatgg cggccagtaa
    52201 acctgcaact ggatgcctgc ccaccgccag aaaaatcagc gcgcccatcg gtggcatgat
    52261 cactaacgcc gcatcggatg aaatatggct aaaaaaagca ataaacagca ccatataact
    52321 ggcgtagcgg gcattaacat acgatgccat tttaaccatt aacgctggca gtaagccgac
    52381 gcgttccgcc agaccggcac ctaaaaccaa cgccaggatc gcgccaagtg gggcaaaacc
    52441 gctaaagttt ttaataacat tgggtaaaaa ccagtgtaat ccttccacac tgagcaggtt
    52501 tttcaccacc accggcgtac catcggtcgg gtttttcgca ctgacgccaa aggccgacaa
    52561 aattgccgtc gtcaccatga gtacgataat caaatagata aagagcaaaa aaggatgggg
    52621 aaccttgtta ccaattcttt cgacccagcc atagagcttc ccggattggg aagacgacgg
    52681 tatggatgac atactcatgg gcattcctcg gttgttgtgt ttgcgttgtt gttattttaa
    52741 aggtgacggt gtcacgcttt tcgggatagg gcagtgatac ggttgcgtgt ccgtaacttg
    52801 ctgatgctct tgttggcatt cttgcaatag ccctgaatca aggaagagat tgactgtggt
    52861 tgctgccata gttttcgccg ccagcagcat tcctttatga gcaatagatg ttcgcccctg
    52921 gctaaccagt tgccacgtat gtagcggtgt accgacggca aaacaggggc tgaaacactg
    52981 ggcaacaggc agtttccaac tgacgtcgcc gacatcagtc gatgccgcaa gcacgttatc
    53041 ggtggtggca tatggagcga cttcattcgc cagtaccgtt tcacgatgac gtagtgtaaa
    53101 agccttgccg ttttcgccac cggttgcggc aatattattc agactgtttt gccgatcgtt
    53161 gggggtgagc gtagcctgaa tttgtttcgc aaaagcctgt tcttcggagt tccattccgg
    53221 ggtaccaaaa tgggataggg cctggtacat ggcattttct aaggtgcgat tcgggagata
    53281 actggaacag gctttgtcga atcggcattc aaccgtggtt tcggtcatca atgccgcacc
    53341 ttcggcgatt tttgagaccc gatcataaat atgctgcaca tcggccattt cgggggcgcg
    53401 gataagataa agcacttctg cctgcgcctg gaccacgttg ggcgagatcc cgccgctatc
    53461 tgtgatggca tagtgtacgc gcgctttttc aataatatgt tcgttgagga agttggtgcc
    53521 agtggtcatc aacgttacgg catcaagggc gctgcgtccc aaatgagggg aattcgcggc
    53581 atgtgctgcg atccctttaa agcgccatga tgcctgaatg tttgccagcg tgcgggtact
    53641 gaacataccg gcaaaggctt ccgggtgcca ggtgagtgcc gcatccacat catcaaatac
    53701 cccctcgcgg accatgaacg ttttacctga gccaccttct tcgccaggac aaccataaaa
    53761 gcgcaccgtg ccgccttgct catattgttc cagccatttc ttgacggcta ttgcagcggc
    53821 aaaggcggcg gttcccagca aattgtgtcc gcaaccgtga ccattttcac cgggcgtcgc
    53881 agatataggt tgcgcgcaac ctgcttgctg acttaaacct gccagggcgt catattcccc
    53941 caacagggcg ataaccggtt tgccttgacc aaacgaagca ataaaggcat ttgggatatt
    54001 gcctacgttg cgggtaacgg tgaagcctgc agattccagc gccgaagcca gatgctccgc
    54061 tgaccagaac tcttcaaaac gtgtttctgg atgatcccag atttgatcgg caatatcggt
    54121 ataacgttgg cgatcggctt caatcgcatc gtcgataaaa cgatagattt cctgcatcag
    54181 atacctcgcg tccagggaaa attgagcgcg gtgcgcgcca gcgtttcgac ggcaatagcg
    54241 agaacctgct cgtcaaaatc gaatttttca ttgtgatgac ctgccgccag ctgtgtgcca
    54301 aacaccatgt aggaggcttg cccttgatgt cgctgcacgc gggccatcat taatgtggca
    54361 tcttcggaac ccgcaggcgc ttcaacacgt tcaatggcct gattgacccc cgcgacctga
    54421 gccgcctggc tttgcaacca tgcgacccat tgcggcgaag gagaactggc ggtagcagca
    54481 cccatcagac gagtttcaac gccgacacca tacatggttg ctgcgccctg aatcgcttgt
    54541 tgtgcacggt caaaaacata ttgattaatg acgtcgctgg ccccgcgtgt ttcaactttc
    54601 agcaacgccg aggcaggaac aacattacga ccgcttcctg cctgcataac gcccacgttt
    54661 actctggaag ctccttcgct gtgcggggcg attgcatgca gtgcaagagt tgcttgtgct
    54721 gcagccaaca aggcattgtg accgtcttct ggttttgcgc ctgcgtgagc ggcggtcccg
    54781 gtgaagtgcg cgtcaaattt ggtggttgcc ataaaattat cactgccgca caccacggtg
    54841 cccgcaggta cgccagtgcc aatgtgaacg gcagtaaaat aatcaacatc atctactaca
    54901 cctgcatcga ccatcgcccg cgcgccacgc gtaccttcct ctgcaggttg aaaaatcagt
    54961 ttgatgacgc catgtagtcc ggactcgaac tgtttaaggg tatgtgccag cccaagccca
    55021 atggcggtat gcccatcatg accacaggca tgcatcattc cggcgttaca tgacgcaaaa
    55081 ccgtcgcggt aggggcgatg gctgacatcc cgtccttcac tgagatccag cgcgtccata
    55141 tcgacacgga aagccataac cggaccgggg cgaccggtat ccagggtggc gacaatacca
    55201 gtgaaaccac cttcaaaagc cgcaatccat tgtgctagcg caccctgttg acgagcgcgc
    55261 tcgaattcgc gttgtagagt gagttcatca ggtaatccca tccggctact ttcattaact
    55321 acttcgcggc ccagcgccag tgaatagccg agctggtgca attcttccgc aacaagggtg
    55381 gcagtgcgga attccaccca gccagactct gcatagtgat gaaaatcacg tcgccagtgc
    55441 gataattttg gggcaagcga attaacaaat tgattcaaaa aatccataac ctttcccgtc
    55501 atcagtaaaa agtgtgaccc ggttcacgta gcgatagttt ttacttatcg ctaactgatt
    55561 tttcacagtt ttaaccgttc acaaattacc ctgacacaat catctgcatt aaagtagatg
    55621 ccagtttctt tggtctgata aataacggtt atcggtggcg tcatggcttt tcaggtaaaa
    55681 attcatcaaa ttcgggcttt tgttgaagtg gctcgtcagg gcagcattcg cggagcgagc
    55741 cgaatgttga atatgtcgca accggcactg agtaaatcta ttcaggagct ggaagaaggg
    55801 ttagcggcgc aactcttttt tcgccgtagt aaaggcgtga cgttaactga tgccggtgaa
    55861 agtttttatc agcacgccag tcttattctt gaagagctgc gcgcagcgca agaggatatt
    55921 tgccaacgac aagggcaact ggcagggcag attaatatcg gcatgggggc cagtatttcc
    55981 cgcagtctga tgccagctgt catatctcgt tttcatcagc agcatccgca ggtaaaagta
    56041 cgcattatgg aagggcaact ggtgtcgatg attaatgaat tgcgtcaggg agaattggat
    56101 ttcaccatca atacctatta ccagggaccg tacgaccacg aatttacttt tgagaaatta
    56161 ctggaaaagc aattcgcgat cttctgccgc ccgggacatc ccgccattgg tgcccgttcg
    56221 atcaaacagt tactggatta cagctggaca atgccgacgc cacacggcag ctactacaaa
    56281 cagttgagtg aattgcttga cgatcaggcg cgaacgccac aggtcggcgt agtctgcgag
    56341 acgttctcag cctgtattag tctggtggca aaaagcgatt ttctcagcat actgcctgaa
    56401 gaaatgggct gcgatccctt gcacggacag gggctggtga tgttgccggt tagcgaaatt
    56461 ttaccgaaag cggcctatta tttgattcag cggcgtgata gtcgccagac accactgacc
    56521 gcgtcattaa tcacgcaatt tcggcgagaa tgcggctatc tgcaaagtta aaactgcata
    56581 aaaaaataga gtctgtcgac atccgccaga ctctatagta cacacagcag tgcatccgcg
    56641 tcttaaatcc ggcattgtct cctctgcgcc ggtgactgtg ttataacggt tttaaccttt
    56701 agttgccaat tttcttcagt gacagatttc acgaaaatat aagttacatt attaatatcg
    56761 tgaatgaata atcatgcata agtattttgc ttaaaatatc ggcaatattt ggaacttatt
    56821 actggaaatt tgggtaatac gttgttggac tgacccggtc tggttatcat atcgcgctct
    56881 taattgcggg aggatgtaac atgaaccttg acgacaaatc gctgtttctt gacgccatgg
    56941 aagatgtcca gccgctgaaa cgtgctacag atgtccactg gcatccagcg cgtaaccaac
    57001 gtgcgccgca gcgtatcgac acgctgcaac ttgataattt cctcaccacc ggatttctcg
    57061 acatcatccc gctaagtcag ccgctggagt ttcggcggga agggttgcaa catggggtgc
    57121 tggataagct gcgcagtggt aaatatccgc aacaggcgag cctgaatctt ttgcgccagc
    57181 cggtggaaga gtgccgcaaa atgatgttca gtttcattca acaagccctg gcggatggtt
    57241 tgcgtaacgt gctgattatt catggcaaag gacgggatga taaatcgcat gccaatattg
    57301 tccgcagcta tgtggcgcgc tggctgaccg aatttgatga tgttcaggca tattgcactg
    57361 cgctaccgca tcatggcggc agcggggcgt gttacgtcgc actacgtaaa acagcgcagg
    57421 cgaagcaaga aaactgggag cgccacgcta agcgcagtcg ttgatcacga gacgcatccg
    57481 tggcttatgc cgccagcacg cggttgcgtc catcattttt cgctcgatac aaagcatcat
    57541 caacgcgttt aaacagttca tcgatgcttt catttccttc gtggtgcgcc acaccaatgc
    57601 tgacagtaaa gcgtggtaag cccgaaatac tcacttttgc cacgcttacg cggatagttt
    57661 cagccagcga aagcgcggta tccagtgggg ttcttggtag caataagaca aactcttcgc
    57721 ctccccaacg aaacaccaaa tcgcctttgc gagcgcaact ttcgagagtg cgggcaaggg
    57781 cgcataacac ctcatcacct ttagaatgcc catagagatc gttaatgtgt ttaaaacgat
    57841 cggtgtcgat gagcaacaag ctgtaatcct gagcgatggc gaggtgctgc atttggcctg
    57901 gctcggtaat gtgataaaac tgtcgccgat tcagtaatct tgtcatcgca tcatggtggg
    57961 cagcatgttc cagctgctcc tccagccgtt tttgctcagt aatatcatgc acaatacata
    58021 acatgagctt gtcgccataa atttcaatcg gcccggcata ggtctgcaca tgacgagtcg
    58081 aaccatccgc cagtttatga ataaaattca aaggtttatg accaccgggt aaatgcgaga
    58141 tttcatgcat gataggcatg acgcgacgcc cgagcatatt aatttcccag gtatgtttct
    58201 ggcacatcgt ttcatggtta taaccataga aattgagcgc ggcgaggtta gcatcgacga
    58261 tttggccatc tcgggagggg tcaatcaaca acattggtgc agagttagtc agaaaaaagc
    58321 gcgcataaaa accttgtttt ttgcgctgat aatttgccga gcgactggct tttaaaccca
    58381 gcgttgccgg tgcttcgata ccttcgaaaa taatcaccgg ttctgtttct gtcagctctc
    58441 gcaaaacaag ccgacagctt aatgctgttt cctcttcttt acgctgaacg gtgaggattt
    58501 cgataatatc gtgttgattt tgcagatcgg agaggtattt cggcagttct ttttgtgagg
    58561 agacggaata gggaccggtt cgtagctgac taaacgtgag gtcttgcatc aacagtcgcg
    58621 ccgcgctgtt ggcataaatt aactgttcct caaagggcga aacgatccag acaggactgg
    58681 tgagtaagtc cagggtattg aagttgtgtg taatcattga gatcccatta tttttatcaa
    58741 tttttgttgc tatccgatcg caaaaaagcc acgtcatatg atcagataat tctgataatg
    58801 atagacgcta tttaacactt cacacggttt gtatacggaa aagcattttg ctttttgtat
    58861 tcaatttaga cagaatttta ttaatcattt cagggtaatg gggtgatgag atgttgcgta
    58921 acagggccag aaggctagac tacaaaataa tgcgttgatg atggaggcac tgtggaagcg
    58981 attaagggat cggacgttaa tgtcccggat gcagtatttg catggatgct ggatggtaga
    59041 ggcggcgtta aaccgctgga aaatacagat gtgattgatg aagcgcatcc ctgctggctc
    59101 caccttaatt atgtacacca tgatagcgcc caatggctgg cgacaacacc gctgcttccc
    59161 aataacgtac gtgatgcgct ggcgggcgag agcacacggc cccgagtcag ccgtctcggt
    59221 gaaggtacgc tgattacatt gcgctgtata aacggcagca ccgatgaacg ccccgatcaa
    59281 ctggtcgcca tgcgtgtata tatggacggg cggttaattg tttcgacccg acaacgcaaa
    59341 gtgctggcgc tggacgatgt ggtgagcgat cttgaagagg gcactggtcc gaccgattgc
    59401 ggggggtggc tggtggatgt gtgcgatgcg ttgaccgatc attccagcga atttatcgag
    59461 cagctgcacg ataaaattat cgacctcgaa gataatcttc tcgatcagca aattccaccg
    59521 cgtggatttc tggctctgct gcgcaaacaa ttaattgtga tgcgtcgcta tatggcaccg
    59581 caacgtgatg tttatgctcg tcttgccagt gaacgtttgc cgtggatgag cgatgaccaa
    59641 cgccgtcgga tgcaggatat tgccgatcgc cttgggcgcg gtcttgacga aatcgacgcc
    59701 tgtatagcac ggactggcgt gatggcggat gaaatcgctc aggtgatgca ggaaaattta
    59761 gctcgtcgta cctatacaat gtcgttgatg gcaatggtct ttttacccag tacgtttctg
    59821 acagggttat ttggcgtcaa ccttggtggg atccctggcg gcggatggca atttggattt
    59881 tcaatttttt gtattctgtt agttgttctt attggtggtg ttgctttatg gttgcatcgt
    59941 agtaaatggt tgtaacaaaa gcaatttttc cggctgtctg tatacaaaaa cgccgtaaag
    60001 tttgagcgaa gtcaataaac tctctaccca ttcagggcaa tatctctctt gcaggtgaat
    60061 gcaacgtcaa gcgatgggcg ttgcgctcca tattgtctta cttccttttt tgaattactg
    60121 catagcacaa ttgattcgta cgacgccgac tttgatgagt cggctttttt ttgcctgtta
    60181 tttatcagcg tctacccttt aagagtccac ccaatgaccg gagggaaata tgacgacact
    60241 tatttatttg caaattcctg tccctgaacc gattcctgac gatcctgttc cagtacccga
    60301 tccgatccct cgcccgcaac ccatgcctga cccaccaccc gatgaagaac cgattaaatt
    60361 gtcgcatcgt gagcgtagat ctgcgaggat acgcgcctgc tgactttgcg tcgatgacca
    60421 cgagaataga ttgtgaccgc tttttctacc ctgaatgttt tgcctcccgc ccaactcaca
    60481 aaccttaatg agttgggtta tttaaccatg acgccggtgc aggccgccgc gcttccggcg
    60541 atccttgccg gaaaagatgt tcgcgtgcag gcgaaaaccg gcagcggcaa aacggcggct
    60601 tttggcctcg gcttgttaca gcaaattgat gtgtcgctat ttcaaaccca ggctttagtg
    60661 ctgtgtccta cgcgtgaact ggcggatcag gtggcaggtg aattgcgtcg gctggcgcgt
    60721 tttctgccaa ataccaaaat tttgacgttg tgcggtggtc aaccgttcgg tatgcagcgt
    60781 gattcgttgc aacatgcgcc gcatattatc gtggcaacgc cgggccgttt gctggatcac
    60841 ctgcaaaaag gcactgtatc actggatgcg ttgaatacgc tggtgatgga tgaggccgac
    60901 cgcatgctgg atatgggatt tagcgacgcc attgatgatg tcatctgttt tgcgccagca
    60961 tctcggcaga cacttctgtt ttcggcaacc tggccggaag ccattgccgc aatcagcgga
    61021 agagtgcaac gcgatccttt agcgattgaa attgactcaa cagatgctct gccacctatt
    61081 gaacaacaat tttatgagac atccagcaaa ggcaaaattc ctctgttgca acggttatta
    61141 agcttgcatc agccatcctc ttgcgtggtg ttttgcaata ccaaaaaaga ttgccagtct
    61201 gtctgcgacg cgctgaatga agtagggcaa agtgcattgt cgttacacgg cgatctggag
    61261 caacgcgatc gcgatcagac cctggtacgt tttgctaacg gtagcgcccg tgtactggtc
    61321 gcgactgatg ttgctgcgcg tggtctggat ataaaatcgc ttgagctggt ggtgaacttt
    61381 gagctggcgt gggaccctga agttcatgta catcgcatcg gtcgtacagc tcgtgcagga
    61441 aatagcggtc tggcgatcag tttctgtgcc ccggaagaag cacagcgggc caatatcatt
    61501 tctgacatgt tgcagataaa acttaactgg caaacgccgc cagcgaatag ttccattgtg
    61561 ccgctggaag cagaaatggc aacgttgtgt atcgatggcg ggaaaaaagc caaaatgcgc
    61621 ccgggtgatg tattaggtgc gctgacagga gacatcgggc ttgatggcgc agatattggc
    61681 aaaatcgccg tgcatccggc gcatgtttat gtcgcggtgc gtcaggctgt tgctcataaa
    61741 gcatggaaac agctacaggg cgggaagatt aaaggaaaaa cgtgccgggt gcggttatta
    61801 aaataatgaa atgttgaatt gcagggtgca agagtaaaca tcttcttcgg gattgccgga
    61861 tgtgacgctg gccgcgtctt atccggcctc cataagagta gcccgatacg cttgcgcatc
    61921 gggcgctatc ctggttattt cacttcaacc acattcagcc gtaactcatc caactgattt
    61981 tcatcttctt caggctgcca gcccgctggt tgtagtggga tctcttcgcg atcaaacgcc
    62041 agatcacccc cgttaaccac ttcagaaccg tgggtaatgc ctttgaaatc gaacaggttg
    62101 gtatcgcaca gatgcgacgg caccacattc tgcatcgcgc tgaacatcgt ctcgatacgc
    62161 cccggataac gtttatccca gtcacgcaac atgtcagcaa tcacctgacg ttgcaggtta
    62221 ggctgtgaac cgcacaggtt gcacggaata atcgggaacg cttttgcatc ggcaaagcgc
    62281 tgaatatctt tctcgcggca gtaggccagc ggacgaatca cgatatgttt gccatcatcg
    62341 ctcatcagtt tcggaggcat acctttcatc ttgccaccgt agaacatatt taagaacagc
    62401 gtttgcagga tatcgtcacg atggtgaccc agcgcgatct tcgtcgcccc cagttccgtt
    62461 gcggtgcgat aaaggatacc gcgacgaaga cgagaacaca gtgagcaagt ggttttgccc
    62521 tccggaatct tctctttcac gataccgtaa gtgttctctt caacaatctt gtactcaacg
    62581 cccagcgttt caagatactc gggcagaacg tgttccggga agcccggttg cttttgatcg
    62641 aggttaacag ccaccagcga aaaattgatt ggcgcgcttt gctgcaaatt gcgcagaatc
    62701 tccagcatgg tatagctgtc tttacccccg gagaggcaaa ccatgatgcg atcgccttct
    62761 tcaatcatat tgaagtcagc aatggcttcg cccacgttac gacgcagacg tttttgtaat
    62821 ttgttcaggt tgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt
    62881 ctctggtact aaatggggca tattgagggc aaactttgca actacgataa ccgcgcattc
    62941 aacatggcta cctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc
    63001 atctgcgcag tttcatgccc catctggctg gctataaatg ccgggttcgc tcctgccgtc
    63061 aacagccagc aggcaaaagt atgtcgcgta tggtacggat tacggcggcg aataccagca
    63121 cgttttactg ctgcattcca tctcgctccc aaatagtcgt agtgaaatct gatgggaaag
    63181 ccttgcttag catgctggtg ttgcgctacc cgtacaggat gcggtaacac aggaagaaat
    63241 tagaacagaa actgtggcgg acattgtgca ttcgttgcca ggcggagaaa tagaacgcta
    63301 caacaagtgc agaatataat ttttcgcaag tgccagaata gcgatattat gcagtgtttt
    63361 ttagttttct atcaggggat attttgtgaa acttttcggt gaataaaata attctctgta
    63421 ataaatgatg ggtatggttg gtggttatat ttgcgcatgg a
//
LOCUS       DADSZD010000006        60594 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.006, whole genome shotgun sequence.
ACCESSION   DADSZD010000006 DADSZD010000000
VERSION     DADSZD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.006"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            36..203
                     /locus_tag="J1Z28_000305"
     CDS             36..203
                     /locus_tag="J1Z28_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBA7715175.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(311..1396)
                     /locus_tag="J1Z28_000306"
     CDS             complement(311..1396)
                     /locus_tag="J1Z28_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA7715176.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGSEFLTCGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKSGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDRYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGAQALEVLRGLVLAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKLGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRGGEETILRILRESIASI"
     gene            complement(1408..2712)
                     /locus_tag="J1Z28_000307"
     CDS             complement(1408..2712)
                     /locus_tag="J1Z28_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715177.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGILILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGMSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2724..3077)
                     /locus_tag="J1Z28_000308"
     CDS             complement(2724..3077)
                     /locus_tag="J1Z28_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBA7715178.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(3088..3966)
                     /locus_tag="J1Z28_000309"
     CDS             complement(3088..3966)
                     /locus_tag="J1Z28_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBA7715179.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITSLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGVLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3963..5189)
                     /locus_tag="J1Z28_000310"
     CDS             complement(3963..5189)
                     /locus_tag="J1Z28_000310"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA7715180.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLTDSQCILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLNEFNTYPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSFRFAGDTL"
     gene            complement(5190..6353)
                     /locus_tag="J1Z28_000311"
     CDS             complement(5190..6353)
                     /locus_tag="J1Z28_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBA7715181.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVAQGTDVITVFTLDKAREVSAAAVKAGRVQSVLLKVYSDDDFLY
                     PGQESGFALKVLPEIVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCISLPLLAQYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(6437..6799)
                     /locus_tag="J1Z28_000312"
     CDS             complement(6437..6799)
                     /locus_tag="J1Z28_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA7715182.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6816..7721)
                     /locus_tag="J1Z28_000313"
     CDS             complement(6816..7721)
                     /locus_tag="J1Z28_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715183.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7881..8885)
                     /gene="trpS"
                     /locus_tag="J1Z28_000314"
     CDS             complement(7881..8885)
                     /gene="trpS"
                     /locus_tag="J1Z28_000314"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBA7715184.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8878..9636)
                     /gene="gph"
                     /locus_tag="J1Z28_000315"
     CDS             complement(8878..9636)
                     /gene="gph"
                     /locus_tag="J1Z28_000315"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBA7715185.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMVLYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9629..10306)
                     /gene="rpe"
                     /locus_tag="J1Z28_000316"
     CDS             complement(9629..10306)
                     /gene="rpe"
                     /locus_tag="J1Z28_000316"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA7715186.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10324..11160)
                     /gene="dam"
                     /locus_tag="J1Z28_000317"
     CDS             complement(10324..11160)
                     /gene="dam"
                     /locus_tag="J1Z28_000317"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBA7715187.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11267..12553)
                     /gene="damX"
                     /locus_tag="J1Z28_000318"
     CDS             complement(11267..12553)
                     /gene="damX"
                     /locus_tag="J1Z28_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBA7715188.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12645..13733)
                     /gene="aroB"
                     /locus_tag="J1Z28_000319"
     CDS             complement(12645..13733)
                     /gene="aroB"
                     /locus_tag="J1Z28_000319"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBA7715189.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13790..14311)
                     /gene="aroK"
                     /locus_tag="J1Z28_000320"
     CDS             complement(13790..14311)
                     /gene="aroK"
                     /locus_tag="J1Z28_000320"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBA7715190.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14712..15950)
                     /gene="hofQ"
                     /locus_tag="J1Z28_000321"
     CDS             complement(14712..15950)
                     /gene="hofQ"
                     /locus_tag="J1Z28_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBA7715191.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15862..16266)
                     /gene="hofP"
                     /locus_tag="J1Z28_000322"
     CDS             complement(15862..16266)
                     /gene="hofP"
                     /locus_tag="J1Z28_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBA7715192.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16256..16696)
                     /locus_tag="J1Z28_000323"
     CDS             complement(16256..16696)
                     /locus_tag="J1Z28_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBA7715193.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTSFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            complement(16680..17219)
                     /locus_tag="J1Z28_000324"
     CDS             complement(16680..17219)
                     /locus_tag="J1Z28_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBA7715194.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17219..17998)
                     /gene="hofM"
                     /locus_tag="J1Z28_000325"
     CDS             complement(17219..17998)
                     /gene="hofM"
                     /locus_tag="J1Z28_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBA7715195.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            18118..20670
                     /gene="mrcA"
                     /locus_tag="J1Z28_000326"
     CDS             18118..20670
                     /gene="mrcA"
                     /locus_tag="J1Z28_000326"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBA7715196.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVDDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20837..21397)
                     /gene="nudE"
                     /locus_tag="J1Z28_000327"
     CDS             complement(20837..21397)
                     /gene="nudE"
                     /locus_tag="J1Z28_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBA7715197.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            21717..23852
                     /gene="igaA"
                     /locus_tag="J1Z28_000328"
     CDS             21717..23852
                     /gene="igaA"
                     /locus_tag="J1Z28_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBA7715198.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQEIR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            23917..24585
                     /gene="yrfG"
                     /locus_tag="J1Z28_000329"
     CDS             23917..24585
                     /gene="yrfG"
                     /locus_tag="J1Z28_000329"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBA7715199.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24596..24997
                     /gene="hslR"
                     /locus_tag="J1Z28_000330"
     CDS             24596..24997
                     /gene="hslR"
                     /locus_tag="J1Z28_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBA7715200.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            25022..25900
                     /gene="hslO"
                     /locus_tag="J1Z28_000331"
     CDS             25022..25900
                     /gene="hslO"
                     /locus_tag="J1Z28_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBA7715201.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25963..27687)
                     /locus_tag="J1Z28_000332"
     CDS             complement(25963..27687)
                     /locus_tag="J1Z28_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBA7715202.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSGWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNLLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFKTLFFATDWFIYLTLGLATALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLVLQGKVNLAVSVLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEHSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQSLQKVVSEKS
                     LAENVLIAPGSGKPDVAFWSALIKERYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSARKEWDALDMSLLPRQITKEKLLAAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            28066..29688
                     /gene="pckA"
                     /locus_tag="J1Z28_000333"
     CDS             28066..29688
                     /gene="pckA"
                     /locus_tag="J1Z28_000333"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBA7715203.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29765..31117)
                     /gene="envZ"
                     /locus_tag="J1Z28_000334"
     CDS             complement(29765..31117)
                     /gene="envZ"
                     /locus_tag="J1Z28_000334"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBA7715204.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGATKEG
                     "
     gene            complement(31114..31833)
                     /gene="ompR"
                     /locus_tag="J1Z28_000335"
     CDS             complement(31114..31833)
                     /gene="ompR"
                     /locus_tag="J1Z28_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBA7715205.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            32025..32537
                     /gene="greB"
                     /locus_tag="J1Z28_000336"
     CDS             32025..32537
                     /gene="greB"
                     /locus_tag="J1Z28_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBA7715206.1"
                     /translation="MRIIKQTKGINEMKTPLVTREGYEKLKQELNYLWREERPEVTKK
                     VTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAW
                     VEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASW
                     YVNAIEYVKP"
     gene            32634..34955
                     /locus_tag="J1Z28_000337"
     CDS             32634..34955
                     /locus_tag="J1Z28_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBA7715207.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVNGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGILQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVAADTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35393..35620
                     /gene="feoA"
                     /locus_tag="J1Z28_000338"
     CDS             35393..35620
                     /gene="feoA"
                     /locus_tag="J1Z28_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBA7715208.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35637..37958
                     /gene="feoB"
                     /locus_tag="J1Z28_000339"
     CDS             35637..37958
                     /gene="feoB"
                     /locus_tag="J1Z28_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBA7715209.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCISAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            37969..38205
                     /gene="feoC"
                     /locus_tag="J1Z28_000340"
     CDS             37969..38205
                     /gene="feoC"
                     /locus_tag="J1Z28_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBA7715210.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            38438..39031
                     /locus_tag="J1Z28_000341"
                     /pseudo
     CDS             38438..39031
                     /locus_tag="J1Z28_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(39060..39830)
                     /gene="bioH"
                     /locus_tag="J1Z28_000342"
     CDS             complement(39060..39830)
                     /gene="bioH"
                     /locus_tag="J1Z28_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBA7715211.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVLAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWTHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            39868..40551
                     /gene="gntX"
                     /locus_tag="J1Z28_000343"
     CDS             39868..40551
                     /gene="gntX"
                     /locus_tag="J1Z28_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBA7715212.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            40610..41185
                     /gene="nfuA"
                     /locus_tag="J1Z28_000344"
     CDS             40610..41185
                     /gene="nfuA"
                     /locus_tag="J1Z28_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBA7715213.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            41549..42865
                     /gene="gntT"
                     /locus_tag="J1Z28_000345"
     CDS             41549..42865
                     /gene="gntT"
                     /locus_tag="J1Z28_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBA7715214.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(42976..45060)
                     /gene="malQ"
                     /locus_tag="J1Z28_000346"
     CDS             complement(42976..45060)
                     /gene="malQ"
                     /locus_tag="J1Z28_000346"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBA7715215.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVMGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYLANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTTLKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRSNMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKT"
     gene            complement(45070..47463)
                     /gene="malP"
                     /locus_tag="J1Z28_000347"
     CDS             complement(45070..47463)
                     /gene="malP"
                     /locus_tag="J1Z28_000347"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBA7715216.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKDLESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYLDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            48086..50791
                     /gene="malT"
                     /locus_tag="J1Z28_000348"
     CDS             48086..50791
                     /gene="malT"
                     /locus_tag="J1Z28_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBA7715217.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKTLPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(50834..51850)
                     /gene="rtcA"
                     /locus_tag="J1Z28_000349"
     CDS             complement(50834..51850)
                     /gene="rtcA"
                     /locus_tag="J1Z28_000349"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBA7715218.1"
                     /translation="MKRMIVLDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGMVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGTGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(51854..53080)
                     /locus_tag="J1Z28_000350"
     CDS             complement(51854..53080)
                     /locus_tag="J1Z28_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HBA7715219.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            53269..54867
                     /locus_tag="J1Z28_000351"
     CDS             53269..54867
                     /locus_tag="J1Z28_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-dependent transcriptional regulator"
                     /protein_id="HBA7715220.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYAFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKERPDSPGAVTIIDLD
                     LSRYNAIANRFAEERQQTLDFLKSGIATRNSHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVGRHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDTVEDEINRLRYNWQES
                     RPSTLTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRELFDISRQGKASVNDA
                     DRLRKYLARFGLTWEAMQDQHSSS"
     gene            complement(54849..55607)
                     /locus_tag="J1Z28_000352"
     CDS             complement(54849..55607)
                     /locus_tag="J1Z28_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBA7715221.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(55624..56454)
                     /gene="glpG"
                     /locus_tag="J1Z28_000353"
     CDS             complement(55624..56454)
                     /gene="glpG"
                     /locus_tag="J1Z28_000353"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBA7715222.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGGGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(56499..56825)
                     /gene="glpE"
                     /locus_tag="J1Z28_000354"
     CDS             complement(56499..56825)
                     /gene="glpE"
                     /locus_tag="J1Z28_000354"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBA7715223.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            57015..58520
                     /gene="glpD"
                     /locus_tag="J1Z28_000355"
     CDS             57015..58520
                     /gene="glpD"
                     /locus_tag="J1Z28_000355"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBA7715224.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLNRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDEDGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQRLSLAS"
     gene            complement(58574..59179)
                     /locus_tag="J1Z28_000356"
     CDS             complement(58574..59179)
                     /locus_tag="J1Z28_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715225.1"
                     /translation="MIKFRLYIPPIILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
     gene            complement(59182..59949)
                     /locus_tag="J1Z28_000357"
     CDS             complement(59182..59949)
                     /locus_tag="J1Z28_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715226.1"
                     /translation="MHPTPAMQALIEQIYHIFRRYPAPKQFVVCCEYCLSQQEQKALR
                     NTSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEREILQQFACQYMTDWVSGDEAVELQYKLEMFFRADIALSPLLDAIIS
                     VPGFWSAASLACLLNTYRDGYIRDNQDDIDKAITAQINAWAFNNQSILKERARQAIEN
                     PLKQPEQGTQYQIWEDDWMIDECLCAMYDASSESPGK"
     gene            complement(59952..60530)
                     /locus_tag="J1Z28_000358"
     CDS             complement(59952..60530)
                     /locus_tag="J1Z28_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715227.1"
                     /translation="MDEGEKFAGAAGLEGLSRYDVTLNIGLTSSNVWPGFSLDTATIS
                     RLCACGADFGFDLYISDVPDVQCDLNTTNDFTVQFTAMLNPDERVIIAKRPLKKCDAW
                     IEDVYIFQVFKEAWQFQNDNSLRGFRDKQAELKLYARHYSVENCTEESCWDCNYCIRP
                     SFSLSRSAIIRLNAANARFVYQPFTRDQRARG"
BASE COUNT        14628 a        16560 c        15180 g        14226 t
ORIGIN      
        1 aatattaatt aatatataat acatagggaa tgtaaatgaa caaatttatt aaagttgcac
       61 tggtaggcgc agtactggct acgttaactg catgtactgg tcatattgaa aaccgtgata
      121 agaactgctc ttacgactac ctgctgcacc cggcaatttc tatttctaaa atcattggcg
      181 gttgcggtcc gactgcacag taagctattg acagaaatcg gctaacgccg gtttaattgc
      241 ctgacgccct cggagttatc cgagggcgta tttgtttctg cattcttttc aaaaatatcg
      301 tttacaacga ttaaatgctg gcaatacttt cacgcaagat ccgcaatatc gtctcttcac
      361 cgccgcgatt tgggttaatg cgaatcgcgc aatgttctaa ctgcggattc gcctggcgaa
      421 acgttccgga aagtcgataa aagagcggcg ggatttcata tttcgactct gcaccaaccg
      481 ggtaaggcaa ggcaccgagt ttctgcgcct cttccagcac tctggcggca atcggctgat
      541 gaaactccac aatcaatacc ttcgactgtg cattggcaat gacggcgctt ttcacttccg
      601 ccaccgcgcc gccgttaagc aaagccagca accgttcaga tacccccgcc tgcaccgcgt
      661 gcatcactgg cgcgagcacc agaccacgca acacctctaa tgcctgcgcc ccctgaatct
      721 gactgccgcc ggaatagagc gtggcgcgaa tccggctaat aacatcggca tcgcctacca
      781 ccgcaccaac gccctctggc ccaaatagct tgaagcagga aaatgtcgac acattcgccc
      841 cgcattcgca gccgattcgc gctaccttca tcaccgcata gttgtcatcg gttaacgctg
      901 gaacacctgc cgcgcgcaac gttgccagca cgtctgccag cacatagcgg tcctgcggct
      961 gctggcgcgt atgctgcacc agcgctgcat caggttgttg ctcgtcaaca acctgcttca
     1021 gtgccgacag atcattgaaa tcagccgtaa tgagcgttag ccccatctgc tcaataataa
     1081 cccgtgtcgt cgggtaaaca ggcgcgtcat gcaccagaag acgctgcccc gatttgagca
     1141 aagccgccag tgccgcacgg atcgcgccag tacccgcgcc ctgtaccagc gccgcagcct
     1201 gtgcgtgaaa tgcatcagcc agcacctgtt ccacacgctg ggtaatgcgg ggctgattta
     1261 gccccggcgt taagcccaaa tcaccgcagg taagaaactc gctgccagga aaatgacggc
     1321 aaatcgtgtc caccagcgca aactgctttt gctgcgcctc aataagcgtc aggctttgca
     1381 gaggaaacgt cttcataggt gccttcctta agccggaacg aacaggccaa gccagtaaag
     1441 aacattcagc aggatgccgg tgatcattac tgctacgacc ggtgccgcca ttttctgtac
     1501 cggacggccc agggattcgt tgaggaagta aatggcaacc gcgatagaga atccggtata
     1561 gcccgccatt ttgattgccg caaagatcga accgaccagc aacgccactt ccatcagcat
     1621 attcatggcg ttacggatgt tatccgacgc attacgtacc gacgggtagc gccccaacca
     1681 tttgccgatc gaacgaagca gcaagacttc cgccgaaata accactgcgc ctaataccgc
     1741 tgcaaccatc ggattcggcg agagatagcc caccgcataa acaaaggtaa agcccgcaac
     1801 tgcatacaca ccggtggcta acgcggtggt ggcaatcaac ggcacaaaac ccagcccgcg
     1861 cataaattct gccagtgccg cctggttgat tagtgtttgc gattgttccg gcgttacgcc
     1921 tgcggagtac gctttctcca gtgtgaagat cgacacttca ctaccggcga aaatcttcat
     1981 gctggcaacg gcagcaatca atgcacccac gatggcgata tacggtaagt ttttgataat
     2041 ccgtgatgta cgctcttcaa acaccgacat accgctggca tcgatatcat tttcatcacg
     2101 atggcgcagg tcatgagtta ttgcgatccc cagcagcatc accatgccaa taaagatttc
     2161 gatggattca gggttaagat gtgggaaata gcgcactacc accacacgag tcatcagtac
     2221 caccacggcg gcgatcagac tttgcttcca gccaaactgg tagaaaatcg ccaccaacgg
     2281 gaacagtgca aaagctgaaa ccaccggcga gcttaattcc cccaggctac ctaatacatc
     2341 caccggtagc gcggtcagca gctggtttac tggcaacaga caggtgagga tcaatattcc
     2401 ccagatagca ccaagaccaa acgccatcag gctgtttatc gccagtacgc cgaggatatc
     2461 ggtaggaaga aagagtaacc atgcgttgag cagcccggtt ttcagggtaa aagagatccc
     2521 caccgaggcc acaaaaccaa tgctcagacc aaaagcgata ctccccgcct cgcgacggtt
     2581 catatagcct tcaatcagtt gcggcaggat cgggcggata ccgtcatgaa aaacggctgc
     2641 cgagcgatgc gccagaagcg atgtcatacc cgtcaggcac gccaccacga taatctgaat
     2701 atacagatcc atagtcgtgc cttttatttc agatggttaa tcagcatcgg aatcgcgtgt
     2761 tcaacgtgct caacggaaag gccaaacgcc acttttcctt cagcgatcat tttggcgata
     2821 tgttcgtctt tcgctttaat gcccggcttg gcaatggtgc aacttttgtt atagccaatc
     2881 accgcgatag caatcgacaa ggccgcgccc gcaccggtat tacacgcgcc gatgtagtaa
     2941 tccagttgcc cggatttcac tttcattgcc gcttccatgt cgttgtgaat gaaaacttca
     3001 aaacagccag gagccgtcgc ttcaatggtt tttttaatct gctcacgctg taagcctgca
     3061 acgccaatct ttttcatgag tctgtcctta ttggaaaaat tgagagggat tttcacgtaa
     3121 catcacatcc acatcggcct gactgaatcc tgactggcgc aattgcggaa taaaagtggt
     3181 caataaaaag tcatagccat aaccaccgtt ggcttttaaa tgagagcgcc gcgtaatatc
     3241 catcgacagc atgacgcgat tcagcacccc acggtcacgt agcgcatgga gcatcgcaat
     3301 gcgcttttcg tccgggtagt aactgttctt gccgatggtg tcgaactgca catacgcgcc
     3361 gagatcgatc atcttcaaaa tgttgtcgag attgtctttc agatcgcagt ggccgacggt
     3421 gacgcgcgaa agatcaactc cgtgggcttg caacagagcc agttgctcca gccccatcgt
     3481 gctgaacgac gtatgcgtgg agatcgggcg tccggtttgg ttatgtgcca gcgcggcggc
     3541 aataaacacc ttctcttcca gcgacgtaat cttcccttcg ctggagccga tctccgcgat
     3601 aattccggct ttcaggtcag tgccatcgat accctgctca atttcatcga ccatctcctg
     3661 cgccagttcc tgcacgctgc gggtcgccac atgttccggg aaaaacgcgt cctggtaata
     3721 accggtacag gccaccacgt tgatcccggt ttcgcgcatt acatcgagca taaattgcgc
     3781 attgcgcccc atgtaacggt tggtcatctc aatcacatta cgcacgcccc gggccatcag
     3841 gtcgttcatc tcctggcaaa tgaacgcata ctgatcaagg cggcagtcca cgttgttttt
     3901 aaagccggaa agatcaatat gcagatgctc atgggcgagg gtgtagcccg tcggatcaaa
     3961 actcataggg tgtctcctgc aaaccggaag gaggaaagaa aagagcgacc attttgtggc
     4021 ggtggcgcac ggaaaaattc acacacggta gcccccacat cagaaagcgt ggtgcgcaca
     4081 ccgagctgcg tagcgaccag tccttgctga taaaccagca ctggcaccac ttcgcgggta
     4141 tggtggctgt gaccaatggt cggatcgtta ccgtgatccg ccatcacgac caggcaatca
     4201 tctggctgca tcgcctcaac aagccgggca aggttacggt caacgacctg caaacgttcg
     4261 gcataacgtg cgacgtcttc tgcatgacca gcgaggtcgg tttcctggat attggtacaa
     4321 ataaacgccg tcggataggt gttaaattcg ttgagggtga tatccataat ccgctggcta
     4381 tccaccaggt tttgccagct cacgccataa ggattgctga cgatatctgc caccttaccc
     4441 accagcacgg ttggcacgcc cgcttcatac agtttttgcg gtacctgcac tttttcatcg
     4501 acaccgtagc ccatatgtac gacctggaaa ccgttgtcat aggcgccaga acgcggcgca
     4561 ttgatgccaa taaagcgccc ttctttgctt tctgcggcat cgaggatgca ctggctgtcg
     4621 gttaacaggc caccaaatgt aatgacccga ccgacctgta cctgctcacg cacgatacga
     4681 cccattttga ttgcgtcgtc aaaagagatc acagagagat tggcggtgat gttatagacc
     4741 tgacctaaat ccgcctcgag attatcgcca atcgcaaccg cctgattgac ccacagaaat
     4801 tgcagatcat cgccacggcg ctccacctgc cagccagcgg aaactaatgc ctgctcaaca
     4861 cggtcaatca catcgcgaaa gggcatccgc agcggcggta acgggcgcgt gcctaaaatt
     4921 tcctgatgcc ccataaaggt atcgccacct tcatgttgca gctctgccac gccccaggtt
     4981 gcggagtctg acggctgcat atcgcctggc gcataaccca atgcgttgat tagccccagc
     5041 ttctccagcg ttggtagctg caaatgcggc aactggctca ggatgtgacc acaggtattc
     5101 gccccggcat cctgcggacg caccagcgtg acatctttca ttgcgcctac gccaaagcta
     5161 tcaatcacta acaccacaaa tcgcgccatt tacgcctcca gagggttgcc gaggctgtca
     5221 taacgaccaa ctatttctgg ttcgccgcgc tgaataccag acaccagcac cacatcgcta
     5281 cgggtgacaa aaatctgcgt gcgaaaacag agcactactg cactgcttac cggaaactcg
     5341 cctgccagcg gcagggtgta gtcgatactg ctgtcatcca cagttttgag attggtttca
     5401 gtaatctttt gattttctgg cgtaaacacc agcgcatgtt gcgcgtgacc acgacggtag
     5461 taaccgccgc cgtagcagta gctgtcgcca cggaaatgat gggagatttc acttaaccag
     5521 agcatcgcga tacgttcagg ctgatcgccc tgctggtttg ccggaatagt gcccgtcagc
     5581 gcatgaccgg gttcggcatg agtcacaccg tattgcgcca gcaatggcag cgaaatgcag
     5641 ctggtcgctg aaggcgcgtt cagttgctca atcgcaatac cagatttcgc cagttgatcc
     5701 cgtgcctgta ccagcgtgtg aagattcggt gtcggcaaaa ctttcccggc agcctcatcc
     5761 caaagcaggc aagggaaatg ggtaagtccc gccaaatgta gccctggcag attctggatt
     5821 tcggcgacaa tctccggcaa caccttgagg gcaaaaccgc tctcctgacc cggataaaga
     5881 aaatcatcgt cgctataaac tttaagcagc acagactgga cacgtccggc cttcaccgct
     5941 gccgcagaaa cttcccgcgc tttgtcgaga gtaaacacgg tgatgacgtc ggttccctgt
     6001 gcaacggcgt cagcaacctg atgacaaggg atttgtacca gatgcccctg atgcgccacc
     6061 ggcaaaccag cgcggcgcat gactcgcgcc tctttgtaat ccaccgccac aatgccgctg
     6121 tagcccagag ccaacagttt ttctgccagc cacggattac gaccgaattg tttggtcatc
     6181 agatacagtt caatgccgta aagccgcgcc gtctcaatca gccgcttgcc gttttccaga
     6241 acttgatcca cgtcgatcac ccaactgtcc ggggcgatct tgccctgctg ccacaaactt
     6301 agtgcggcgg aaatcagcgc cgggttctgg cgtttcaatg cttctacaaa catcttgttg
     6361 cctcgtttct gttccttgaa ttaaatattc atttttttga atatttaggt tgtgcgacgt
     6421 ggatttatcg ggagcttcaa acctcttcgt tagcagccat ccataatcca taaaggttgg
     6481 ccagcaaata gccttcttca cacgggttaa cttccagtgc aaattccttc aacaacacct
     6541 gatgcaattg cacaacggct tgccagtggc tggattgcgc cagttccgcc agcaattcgt
     6601 catccagcgg ctctatttct tcaccacggc gactgcgcat cagtgcactc gccatatgtg
     6661 tcatcgccat cgttccttgc tcactgcgca ccggtagatg gcactctgtt tccagtacat
     6721 tgacgacctg catcatgcct ttgcaaatgt ccttatcaat aacgcctgca tcgcaaagca
     6781 ggttgagtct ggtttccata tcagatgcct ttcgtttaat aactgggttc ctgttcccca
     6841 ctgactactc gctgttgatg agcaagcagg gcatgtttat caacaactgc acgcagcagt
     6901 tgttgcattg ggtaatcatc gactcgcgtc aggatcacgg cttcggtggc ctgtaaaaat
     6961 cgcggatcgt ctgtgagcgg cgtcgcttct aatcccagca tggtcagttc gttttccgcc
     7021 actacgttcc agataaccgc gtcaacatcg cctttaacaa tgcgttgtaa actctcgtga
     7081 taagagagat cgactcgttc cacatcacta tcgccaaaaa agacatcggt catgattttc
     7141 tgatccgccg aacggttatc cagccccacg cgcttcacgt ttgcggactc gcctttacgg
     7201 caaatcaact ggtgctcgcc aacgtaggta tgcggtccca actccagcgc gaggcataat
     7261 cctttttgcg tgagataact ttccgccgcc agtcgcgaaa ccaccgccat gtcatacacg
     7321 ccgttaagta aacactccac gcgaatatcc gcgccgcgca tatgcgcata gtaaaaagga
     7381 atgccatcaa actgggcttt caatccgctc gccaggcctt cgtacaaacg ggtatagggc
     7441 aagggcatcg cacataccac gttgttgata tccacatgag tcagcaatgc tttgttatcc
     7501 atctcgacca gataactgcc attgcgccca cggcgttcaa tccgtatcgc tccgcttgat
     7561 tccagcgttt tcaacgcggc ctgcgtcagg ccaaccgatg aacggcattc agttgccagc
     7621 tcatctatgg ttttcagtcg attaccgcac ttttcaccca acaaataacg ggccagtgtc
     7681 gtgataacga cgccttcttt tttgataaac gttcgacgca taatatattt tcaataaaat
     7741 gaatgtttat atcttcagga atttgaagat aaggcgcaac agcgagatgt ggaaacggcg
     7801 aggcgcttca cattttttcg gattattgca aatgaggata aaaaaaccgg gtttccccgg
     7861 tttttggcat taatgttaaa ttacggcttc gccacaaaac caatcgcttc gtacaccgct
     7921 tttagcgtac gggaagcgtg tgcgctggct ttttctgcgc cgtctttcat cacctgttgc
     7981 aggaaggctt catcgttgcg gaaacggtgg taacgttcct gcaattcagt cagcataccg
     8041 gaaacggcat cagccacttc acctttcaga tgaccataca tcttgccttc gaactgtttt
     8101 tccagttccg ggatgctctg gcccgttacc gcagaaagga tatccagcag gttggaaacg
     8161 cccgctttgt tctgcacatc gtagcgaact accggcggct cgtcggagtc agtgaccgcg
     8221 cgtttgattt tcttcactac cgatttcgga tcttccagca ggccgataac gttattgcgg
     8281 ttatcgtcag acttggacat cttcttggtc ggctccagca gcgacattac gcgcgcgcca
     8341 gatttcggaa taaacggctc tggcacctta aagatatcgc cgtacagcgc gttgaaacgc
     8401 tgggcgatat cgcggctcag ttccaggtgc tgtttctggt cttcacccac cggcaccaga
     8461 ttagtttgat acagcaggat gtccgcagcc atcagcaccg gatagtcaaa cagaccagcg
     8521 ttgatgttct cggcataacg cgcagattta tctttaaact gcgtcatgcg gctcagttcg
     8581 ccgaagtagg tatagcagtt cagtgcccag cctaactgcg catgttctgg tacgtgggac
     8641 tgaacaaaaa tggtgctttt ctcaggatcg ataccacaag ccagatacaa ggccagcgta
     8701 tccagcgtcg ctttacgcag cttctgtgca tcctggcgca cggtgatcgc gtgttggtca
     8761 acgatacagt aaatgcaatg gtagtcatcc tgcatgttta cccactgacg cagcgcaccc
     8821 atgtagttac caatggtcaa ttcacctgag ggctgtgcgc cactaaaaac gatgggctta
     8881 gtcatttttc gattcctgat tttcgctatg cggaagccct aatgcgggca gaaggtcatt
     8941 tatagactga taaattacat caggctggct gagatcgata gcctcgccgt agttatatcc
     9001 gtaggttaag ccaactgatg gacaacctgc cgcttttgct gcctgaatat cattgcgtga
     9061 gtcgccaaca aacagcatct gttgtggagc aatgcccatc cgctcagcca ccagtagcag
     9121 cgggtccggg tgcggttttt tattttgcac atcatcacca ccaatcacca cgctgaagta
     9181 tttggcgata tctaaggctt cgagcagcgg cgcgacgaac ggcgtcggtt tgttggtgac
     9241 caggcccagc ggcaggcctt tagcctgcaa cgcgcccaac gtatcggcaa cgtgcgggaa
     9301 caaaaacgtc ccctcttcgg caacctcgcc atagtagcga tcgaacagtt tacgcaggat
     9361 acgtacctgt tcttctgccg gaatgtcgtc atcaacgggc ggtttaccca ttgttttacg
     9421 cagagtcgca cgttcctgac gcgcccaggt caatgcgcgc tccatcagaa tatctgcgcc
     9481 gttaccaatc caggtaataa cgcgttcttc acctgcgaca ggcaactcca gcgcatacag
     9541 caccatatct accgcagcag caagaccggg tgcactgtca accagcgtac cgtcgagatc
     9601 aaaagcgacg ccgcgaatat cttcaaactt attcatgact tacctttgcc agttcactgc
     9661 gcatttcatc aatgactttt ttgtagtctg gctggtcgaa gattgccgaa ccagcaacaa
     9721 acatatccgc gcccgccgca gcgatttcgc caatgttgtt caccttcacg ccaccgtcca
     9781 cttccagtcg aatgtcaaag ccagactcgt cgatacgacg acgtacttcg cgcagtttat
     9841 ccagtgtttg aggaatgaaa gactgaccgc cgaaaccagg attgacggac atcagcagga
     9901 tcacatccag cttatccatc acgtaatcca gataactcag aggtgtcgcc gggttaaata
     9961 ccaaacccgc tttacagcca ttttctttaa tcagttgcag cgtgcggtca acatgctcgg
    10021 aggcttctgg atgaaaggta atgatgctgg caccagcggc agcgaaatca ggcacaatgc
    10081 gatcgacggg tttcaccatc agatgtacgt cgataggggc ggtaatgccg tagttacgca
    10141 aggatttcag caccattggc ccaatcgtca gattgggaac atagtggtta tccatgacgt
    10201 caaaatgcac gacatcagcg ccagctgcca gggcttttgc ggtatcttca cccaggcggg
    10261 caaaatcagc cgacagaatt gagggggcaa tcaaatactg tttcatccgc ttctccttga
    10321 gaattatttt ttcgcgggtg aaacgactcc tggtttgtac aaagccagca gttcgtccac
    10381 ctttttacgt gtgccgccgt tgctgcttat actgcgtcga actttgacga catgcaattt
    10441 tgcgcgctga taccactcac gcgttaacat cgtatcgtga ttggagatca gcactggaat
    10501 atggcgatca accagacctt cagcgatctc cgccagatgc gcttgttgtt caagcgtaaa
    10561 actgtttgtg tgatacgccg taaagttggc agtcgcagac agcggcgcat aaggcggatc
    10621 gcaatagacg acagatgcat catctgcgcg cgccatgcta tcggcgtaag actcacaata
    10681 gaaaaaggca ttctgcgctt tttcagcgaa gtgatacaac tctgcttccg ggaaataggg
    10741 ttttttgtag cggccaaacg gcacgttaaa ctcaccgcgc agattgtaac gacacaggcc
    10801 gttgtaaccg tagcggttca aatataaaaa cagtaccgcc cgacggaacg gatcctggct
    10861 tttgttgaac tcttcgcgga actgatagta aacctcggcg caatttgttt cgggaacaaa
    10921 cagctcgcgt gcggcctgta cgtactcatc agtacgcatc ttcacaatgt tatagagact
    10981 gatcaggtcg ctattgatat cggcaaggat ataacgagaa aagtcggtgt tgagaaacac
    11041 cgacccggca cctacaaaag gctcaaccag acattcgccc ttgggcaaat gccgtttaat
    11101 atcatcaagc aggggatact tgccccctgc ccacttcaaa aaagcgcgat ttttcttcat
    11161 gctgactaac taattacacc ttctccggct gtggagaaag ctccgacagc atccagcgct
    11221 tcaggcaacg cccgcaatga taaaccattg cgggagataa ccttgattac ttcagatcgg
    11281 cctgtacctg acgcagcggt ttcgcccacg ggtttttggc ctggacatct gctggcaatg
    11341 tagataccgc ttttttcgcc tcttctttcg aagcatacac gccagaaacc aggacatacc
    11401 acggctgacc attacgcgtc gtttcataga caacgtagtt tttcagattc tctttcttcg
    11461 cccaaccgtt caggttgtcg tagttagagg aactgctcag ctgcagagtg taatggctgg
    11521 acggtgccga tttcaacgaa ccaacattac ctgcggtctt ccctccagcg gctggtgttg
    11581 ccgtggtttg cgccggggat gccgtctgta ctggagccgt agtcgccgtc tcttttggtg
    11641 ctggcgtaga agtcgcagcc ggtgcctttg tgctcgccac aggggcagct ggttcagttc
    11701 gcttcggcgt ctgtgcgact ggcttcggtt ccgttttcac ggttgcttgc ggttttttcg
    11761 gttcaatcac cgcctgctga cgagccgggc gcgtagtggc cggacgttca gcggtttgcg
    11821 ttttcgccgt gtcacgcgat gcattgccat tgcgtactgg cgcaaccgtt gcgggttcgg
    11881 tcggcaatgt agaattgacc gccacattat tcagctgttg ctgattttgt ggctgggtca
    11941 gcgcattgtt caggtcaccc tgcacttcaa cacgttgctg accatccgtt gccgccgggg
    12001 tttgcccttg agtcggcgta gaagagatcg gcggcagaga aacatcctgc tgagtatttt
    12061 ccgcagacgt ggttcccggc gctggctgca cgccattggc ctgatcggtc gcattgcctg
    12121 caagatcaat actcttctcg ccagacgcgg tttgatcgct ggaagaggtc gagggggctt
    12181 ttagcgcaga accgataccg atgatcaaca gcagtagaac cagaatgccg acacccatca
    12241 tcatatactg acgagaagcg ggtttactgg ctgctttttt gcgcttacgc ggacgacgct
    12301 ctacgcgctc ttcatccacg gtttcatctt cggattcgtc aatttcttct tcgatttccg
    12361 gttcctcatt acgcgctttt tgcgcacgag tcggacggcg atcgtcagtg tcatcaagtt
    12421 caatatcatc aaaattgatc tgcggttcgc cacgttcagt acgctcagaa cgttcagaag
    12481 attgacgaga acgaccagta cgacgatcgc tgggatcggg tttcagctcg tcttctggtt
    12541 tgaattcatc catttaacac cccactaaaa ggttaatgct taccacgttg caattaacct
    12601 gaagctaata gaccgcttga taagcggcct gacctttctt gttgttacgc tgattgacaa
    12661 tcggcaatgg cgttaagaac aagctcgtgc gaaacgccgc tgcgaacttc actcttacca
    12721 attgccaacg gaagaattaa gcgcatctct cccgcaagga ctttcttgtc acgcagcata
    12781 tgcggtaaat acgcctgcgc ggacatttcg cgcggcccat tgaccggtaa cccagcccgc
    12841 gtgagcaggg ttataatacg ctgcgtttcg gcagaactaa actgcccgag acgttccgac
    12901 gtccgcgccg ccatcaccat acccgcagcg accgcttcac catgtaacca attgccatac
    12961 cccatttcag cttcaatggc atgaccaaag gtgtgtccca gattcagtaa agcacgtaac
    13021 ccggtttcgc gctcgtcggc ggcgacaact tctgctttca gctcacaaca acggcgaata
    13081 cagtacgcca ttgccggacc gtccagacgc aacaacgcat ccagattctc ttccagccag
    13141 ttaaaaaacg caccgtcaag aataatgccg tatttgatga cttctgccag ccccgacgct
    13201 aactcacgcg ggggaagcgt tttcagacag tcgagatcca ccaccactga agcgggctgg
    13261 tagaacgcgc caatcatgtt tttaccgagg ggatggttga ccgcagtttt gccgccaacg
    13321 gaggaatcga cctgcgacag taacgtcgtc gggacttgaa tgaaacgtac accgcgctga
    13381 taactcgccg ccgcgaagcc ggtcagatcg cccactacgc cgccgccaag cgccaccaga
    13441 gtagtatcgc gaccatgcgg cttttgtaac aatgccgtaa agacggtatc gagtacagcc
    13501 aggcttttat actgctcgcc gtcagggagg ataacgctat cgacgttaac acccgcctgt
    13561 tcaagtacgc cgcggacctt atcgagatac agaggagcca gggtttcgtt ggtgaccaac
    13621 atgacctgct cgcccgattt cagcggtaag aatgaagctg gttcattaaa caaaccagat
    13681 gcgatggtaa ttgggtaact acgttccccg agagtaacga caatcctctc cataacgcga
    13741 catccccctt aattactgta cccgcagacg agtgtatata aagccagaat tagttgcttt
    13801 ccagcatgtg aataatctgg tttgcaacca ctttagcgct ttgatcatca gtacgaatgg
    13861 tcacgtcggc aatctcttca tacagcggat tgcgttcatt ggccaacgct tccagaactt
    13921 cacgcggcgg tgtttcaacg tgcagcaacg ggcgtttttt atcacgctgc gtgcgtgcaa
    13981 gttgcttttc gatggtcgtt tcaagataaa cgacaacgcc acgagcggaa agacggttac
    14041 gcgtttcacg ggatttcaca gagccgccgc cagtagccag cacaataccc tgtttctcgg
    14101 tcaactcatt gatgaccttt tcttcgcgat cgcggaagcc ttcttcgcct tctaaatcga
    14161 aaacccagcc cacatcagct ccggttcgtt tctcaatctc ttgatcggaa tcgtaaaatt
    14221 ccatattgag ttgttgagct aactggcgcc caatagtgct ttttccggca cccataggcc
    14281 caaccagaaa gatattgcgt ttctctgcca ttttttcggt actactaaga ctattcgtta
    14341 atgataaacc cgcttcgctc agagagcgcc gcaggacatg aactgaaacc tcataagata
    14401 ttgcgagagt cagactgaaa attatctcaa tactccagcg ggtttggcaa ctgaataaat
    14461 cacccatccc tctgcatatc tggtcgctgc aagcgcgctg ccttgctacc accgctctgg
    14521 cgataaatca ccgggtaaga ttagcgtaaa aaagacagca aaatgccgcc tgaatgataa
    14581 atcatcatca tggaacgcag gcagcgaaga gcaccaactc tcaaatcggt actccttgta
    14641 tgctaaatac ctgcgcgcgt caaatagatg aaacacgttc agcgtaaaaa cattaccgct
    14701 tttacggctg tttactcact ggaaaccagt cgtggcgtga taaacaccac taactcgcgt
    14761 cgttcatctt cttttccgtc atgacgaaat aattgcccga accagggaat gtcgccaagc
    14821 aacggtacgc tatcctgacc cgatttattt ttacgggtaa aaatgccgcc cagcgccaac
    14881 gtttctccgc ttttgacctc gacctgcgtt tcgatctcct gcttatcaat cgccagcact
    14941 tcgccatctg cctgctgtag cacctgcccc ggaacgttct ggctgatgtg taatttcagc
    15001 cggatgcgac ctttttgtaa caccgtgggc gtgacttcca tccccaggac ggcctcttta
    15061 aattccaccg acgtcgcgcc actttccccg ctggaaacct gatatggaat ttcgctcccc
    15121 tgtttaatgc tggcaggctg aagatgtgag gccagcagac gcggactggc gataatatcc
    15181 agctgctgtt tttgttcgag cgcggaaagc tcaagatcca gtaaacgtcc gttgatgcgt
    15241 ccaatgttaa aaccgatatg cgttgtcgcc gtcgctacgg agaggtcgct gccaagcgtg
    15301 gtgacttgcc caacgccacc agcttgttgc gcatcggcca gcgtccattt cacacctaac
    15361 tcacgcaaac ttttttcatt aatggtgaca atatgcgccg agagctcaac ctgcccgacc
    15421 ggcagatcca tttgcgatac ccactgttca agcgtgctta acgccgtttt gttatctcgc
    15481 agcaaaaggc gattggtgcg tttatcgacg gtcatactcc ctttggcact cagtagcttc
    15541 tcccccgctt tcgccagctc tccggcgtcg gcgtattgca gagtaatatt gcgattttcc
    15601 agcggcagat ttgcctgcgc ccgcgtctgc tccgcctcct ggcgggcgat attgtcattc
    15661 tgccaggcaa cggaatgcac cgagagaatg ttgccctcct ggcgcgttat caatccggcg
    15721 cttttcacta cagtttgtag tgcctgcttc cagggaacgt cagttaaatg taacgacacc
    15781 gtaccgctga cgtctggcga aaccaccagg ttcaacttct cctgttcagc cagcgcctgc
    15841 aacacctgag ctaccggaac gtcatccacc atcagcgtca ctttttgcgg ctttgccgcc
    15901 tgtacgccgg gtatcagcat caacagtagt gcggctatcc attgcttcat ttgtatctcc
    15961 ttgtcgttgc cacaaccagt gtggcggttc gcagtttgtc ccggttccca gcgttagtgc
    16021 gtctggcgtc agctgtaaga tcgtccagcc gttttccagc acatcgtttt gctgcacccg
    16081 tcgccatttt ttttgcccat cttttattac accgatgatg cgctcgcctc gccctaccat
    16141 cccctgatag cgccactggc taagttcgct aatccggcat agatcttcgg gcggtttaaa
    16201 agggtcacgc ataccggtta aaaggcacaa tgcaatgcct gctaacaacc agcgtttaac
    16261 cctcatgcgg cgtctccagt tgtagcgtga acaaaaggtc atcaccttcc acgcttaaca
    16321 aaaaacggct cacgctgacg ttgcgctctg ccagccgtgt aaatgccgac ggcactgctt
    16381 cccacagcgt tttcaacgcc aactcacctc cctgcgcgga tggatgccag taaaccagtt
    16441 gcgcgccgga taactgaaaa tccagtggcg aaaagggcag cgttttttct tcgctgaagg
    16501 aagtggtgtc taccaggcga tacagtgcag cccactgctg gtgatgattt gcccgtagtc
    16561 gaattaatgc atcgctctct tcatggtgtg tcaatgacag aaaaatgagc gtaactaaca
    16621 ccagcaacca gactgcccag caaaactggc ggaggcggag tgatgtggcg aaccaccagt
    16681 caaagaacat gttcatcgct aaccttcctt gttaactgat actcaaattg ccagcgtccc
    16741 tgcgcatcct gctgcgtggc ccccctctga ttgagatgaa aagaagcatc ctggcggagt
    16801 gacgtttcca gcgcgtttaa cgcggcaatg cttgttgtca gccccttgat ctccagcgtt
    16861 ccctgctgcc agcttatcgt tgtcagccag gcatgttcgg gtaaaagcgc cgccagtgct
    16921 tccagcgcag attgccagtc gcgggtaaat tgtcgctggc gctgcctttg caaacgctgt
    16981 tcgcgtaact gctggcgttc cagcaaacgt ggtttcgtta tctgtaagct gcgggcgagt
    17041 tgttgttccg cctgaagcaa aacggcgttt acgcgtgctt cggcattgct tgtcagacgc
    17101 agtattagcg ttatcccgac ggccagcagc agaggcgcaa cgaacatcag caaccagaaa
    17161 cgaagaaaag cagtccggcg ttgctgtcgc cagggcaaaa aattaattgg cgggttcatc
    17221 agtacacctt cccaagcgcc agccccagcg cgatggcaaa gtctccacct ggtggcggta
    17281 gcggcggctg acgaacagaa accgcctccc aggggtcaaa tccgccttcg ccacatagcg
    17341 cgacgctttc gggatcaaca gataacgctg ccgccagctc cttcgcgcta gtcatcccca
    17401 ccgccagttt gcgcccccag ctatagcgcg tcgcccacag ccactgttcg ttatcacgcc
    17461 aggccagaca ttgctgatga gaaggtaaaa aaggcaggaa tcgctgtaat gcactggcat
    17521 ccggagtgat cgcgctaaca tgaacacgca acctttctgc cagagtaagc agcgttgcca
    17581 gctctttgct ttgcgcggca gtcacgttat aggcgggact gagtgagtct tcgctgtaat
    17641 cgaagcgcag ggagtccgga tccatatcca gttcacgggc catcgtcccg gacagccagg
    17701 ccatttgctc ccgctcaccg agggacatcg acgggcgtgg aaatgaccgc tgtaatgtgc
    17761 gactggcggg aaacgccaac ataatgtgat gacgctgcgg cagttcacga ctccacggta
    17821 acaacgtgtt agccagccgc tgcacatcaa caatccgccc atctttgata atgtcgttct
    17881 ccagtggcaa ccgccaccag cggtgcaata agcattcttt tgcgctccgc acgatagcaa
    17941 ccgctaccgc ttcttgctgt tgtaaatgca aaccaatttg ccagttctta aatgccattg
    18001 tgatgatctc cttatcaccc gtcactctga cgggtatatc aatgcgtctg gcttgccttt
    18061 atactaccgc gcgtttgttt ataaactgcc caaatgaaac taaatgggaa atttccagtg
    18121 aagttcgtaa agtatttttt gatccttgca gtctgttgca ttctgctggg agcaggctcg
    18181 atttatggcc tataccgcta catcgagcca caactgccgg atgtggcgac attaaaagat
    18241 gttcgcctgc aaattccgat gcagatttac agcgccgatg gcgagctgat tgctcaatac
    18301 ggtgagaaac gtcgtattcc ggttacgttg gatcaaatcc cgccggagat ggtgaaagcc
    18361 tttatcgcga cggaagacag ccgcttctac gagcatcacg gcgttgaccc ggtggggatc
    18421 ttccgtgcgg caagcgtggc gctgttctcc ggtcacgcgt cacaaggggc gagtaccatt
    18481 acccagcagc tggcgagaaa cttcttcctc agtccagaac gcacgctgat gcgcaagatt
    18541 aaggaagtct tcctcgcgat tcgcattgaa cagttgctga cgaaagacga gatcctcgag
    18601 ctttatctga acaaaattta cctcggttac cgcgcctatg gtgtcggtgc tgcggcacaa
    18661 gtctatttcg gtaaaactgt cgaccaactg acgttgaacg aaatggcggt gatagccggg
    18721 ctgccgaaag cgccttccac cttcaacccg ctctactcaa tggatcgtgc cgtcgcgcgg
    18781 cgtaacgtcg tgctgtcgcg gatgctggat gaagggtata tcacccaaca acagttcgat
    18841 cagacacgca ctgaggcgat taacgctaac tatcacgcgc cggagattgc tttctctgcg
    18901 ccatacctga gcgaaatggt gcgccaggag atgtataacc gttatggcga aagtgcctat
    18961 gaagacggtt atcgcattta caccaccatc acccgcaaag tgcagcaggc cgcacagcag
    19021 gcggtacgta ataacgtgct ggactacgac atgcgccacg gctatcgcgg cccggcaaat
    19081 gtgctgtgga aagtgggcga atcggcgtgg gacaacaaca aaattaccga tacgctgaag
    19141 gcgctgccga cctatggtcc gctgctgcct gccgcagtca ccagcgccaa tcctcaggaa
    19201 gcaacggcga tgctggcaga cgggtcgacc gtcgtattga gtatggaagg tgttcgctgg
    19261 gcgcgtcctt accgttcgga tactcagcag ggaccgacgc cgcgtaaagt gaccgatgtt
    19321 ctgcaaacgg gtcagcaaat ctgggttcgt caagttgacg atgcatggtg gctggcgcaa
    19381 gtgccggaag tgaactcggc gctggtgtcg atcaatccgc aaaacggtgc cgttatggcg
    19441 ctggtcggtg gctttgattt caatcagagc aagtttaacc gcgccaccca ggcactgcgt
    19501 caggtgggtt ccaacatcaa accgttcctc tacaccgcgg cgatggataa aggtctaacg
    19561 ctggcaagta tgttgaacga tgtgccaatt tcccgctggg atgcaggtgc cggttctgac
    19621 tggcagccga agaactcacc accgcaatat gccggtccaa ttcgcttacg tcaggggctg
    19681 ggtcagtcga aaaacgtggt gatggtacgc gcaatgcggg cgatgggcgt cgactacgct
    19741 gcagaatatc tgcaacgctt cggcttcccg gcacaaaaca ttgtccacac cgaatcgctg
    19801 gcgctgggtt cagcttcctt caccccaatg caggtggcgc gcggctacgc ggtcatggcg
    19861 aacggcggct tcctggtgga cccgtggttt atcagcaaaa ttgaaaacga tcagggcggc
    19921 gtgattttcg aagcgaaacc gaaagtagcc tgcccggaat gcgatattcc ggtgatttac
    19981 ggtgatacgc agaaatcgaa cgtgctggaa aataacgatg ttgaagatgt cgctatctcc
    20041 cgcgagcagc agaatgtttc tgtaccaatg ccgcagctgg agcaggcaaa tcaggcgtta
    20101 gtggcgaaga ctggcgcgca ggagtacgca ccgcacgtca tcaacacacc gctggcattc
    20161 ctgattaaga gtgccttgaa caccaatatt tttggtgaac caggctggca gggtactggc
    20221 tggcgagcag gtcgtgattt acaacggcat gacataggcg gaaaaacggg taccactaac
    20281 agttcgaaag atgcgtggtt ctcgggttac ggtccgggcg ttgtgacctc ggtctggatt
    20341 ggctttgatg atcaccgtcg taatctcggt catacaacgg cttccggagc gattaaagat
    20401 cagatctcag gttacgaagg cggtgccaag agtgcccagc ctgcatggga cgcttatatg
    20461 aaagccgttc ttgaaggtgt gccggagcag ccgctgacgc cgccaccggg tattgtgacg
    20521 gtgaatatcg atcgcagcac cgggcaatta gctaatggtg gcaacagccg cgaagagtat
    20581 ttcatcgaag gtacgcagcc gacacaacag gcggtgcacg aggtgggaac gaccattatc
    20641 gataatggcg aggcacagga attgttctga ttaaaaaggc gcttcagcgc ctttttaagt
    20701 ttgctgacaa agtgcacttt gtttataccg gatgcgggtg aacgcgttat ccggccaaca
    20761 aaaaattgaa aattcaataa attgcaggaa cttcgtaagc ctgataagca ttatgcatca
    20821 ggcaaacttc acgcatttac actcgcccct gccctttcaa ccattcgcgc acgaggaaca
    20881 gcgcgctgac attacgcgct tcgttgaagt caggggcttc cagcaaatcc atcatatgcg
    20941 ccagcggcca gcgcacctgt ggtaacggct ctggctcatc gccttccagt gattccgggt
    21001 agagatcttg cgctaccacg atattcattt tgctggaaaa gtaagacggt gccatgctga
    21061 gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac ctcttctttt agctcgcggt
    21121 tagcggcttc aaagacgctt tcacccggat caattaatcc tttcgaaaaa cctaattcgt
    21181 aggattcagt tcccactgcg tattcgcgga tcaggatcag gtgatcgtcc acaatcggca
    21241 caatcatcac tgcttcccgg ttggttggac gcattcgttc ataaacacgc cgcacgccat
    21301 tgctgaactc cagatccacg ctttcgacgg taaacagtcg ggaacgggct acagtttcaa
    21361 cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt gggatctatg ctgtgaaatc
    21421 agcggttatt gtgcgatatc ggacacgctt tcggcaatgt gaattgcgtg ttatttacat
    21481 ttatgtaact taataaataa tcgtcctcaa atcaaattaa aagtcaatag gttgaaataa
    21541 ctccaggaat ttgctgatat tccgccttca gagggtttgc tatgatcagc ggttactgtg
    21601 atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg atagccaacc gcagaatcat
    21661 gtattgtgtc cggtgcgact gaccacgcct gacagactaa gtaagatggg gaaagcatga
    21721 gcaccattgt gattttttta gctgctttgc tggcctgctc actacttgcg ggatggctga
    21781 taaaagtgcg atccagacgg cgtcagctgc cctggaccaa cgccttcgcg gatgcgcaaa
    21841 cgcgtaaact cacatctgaa gaacgtagcg ccgttgaaaa ttatcttgag agcctgacgc
    21901 aggtattaca ggtgcctggc ccaacgggag ccagcgcggc accgatctct ctggcgctga
    21961 atgccgaaag caacaacgtc atgatgctga cacacgctat cacgcgttat ggcatctcta
    22021 ccgacgatcc gaataaatgg cgttactacc tcgattcggt agaagtccac ctgccgcctt
    22081 tctgggaaca gtacatcaac gatgagaata ccgttgaact gattcatacc gattcgctgc
    22141 cgctggttat ttcactcaac ggtcatacgc tgcaggagta catgcaggaa attcgcggct
    22201 atgccttgca acctgttccg tcaacgcagg cgtcgattcg cggggaagag agcgagcaaa
    22261 tagaactgct caatattcgc aaagaaacgc acgaagagta tgcgctgagt cgtccgcgcg
    22321 ggctgcgcga agcgttgctg attgtcgcct ccttcctgat gttctttttc tgcctgatta
    22381 ccccggatgt ctttgttccg tggctggcag gcggcgcgtt actgctgctg ggcgcaggtc
    22441 tgtgggggct attcgcgccc ccggcaaaat cctccctgcg ggaaattcat tgtctgcgcg
    22501 gtacaccccg tcgttgggga ttgtttggcg aaaacgatca ggaacagatc aacaatattt
    22561 cactcggtat tatcgacctg gtctatcccg cacactggca gccatacatt gctcaggatc
    22621 ttggtcaaca aaccgatatc gatatctatc tcgaccgcca cgtagtgcgt caggggcgat
    22681 atctttcgct gcatgatgaa gtaaaaaact ttccgttaca gcactggctg cgcagtacga
    22741 ttattgctgc gggctcgcta ttggtgctgt ttatgctgtt attctggatc ccgctggata
    22801 tgccgctgaa attcaccctc tcgtggatga aaggcgcgca gaccattgaa gccaccagcg
    22861 tgaaacaact ggctgacgct ggcgtgcggg ttggcgatac attgcgtatt agcggtacgg
    22921 gaatgtgtaa tattcgcact tccggtacct ggagcgcgaa aaccaactca ccttttttac
    22981 cgtttgactg ctcgcagatc atctggaatg acgcccgctc attgccatta ccagaatctg
    23041 aattggtcaa caaagcgacg gcattgaccg aagcagttaa tcgccagctg caccctaaac
    23101 cggaagatga atctcgcgtc agtgcctcat tacgttcagc aattcaaaaa tccggtatgg
    23161 tattgcttga tgattttggc gacattgttc tgaagacagc agatctgtgt tctgccaaag
    23221 atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg caacagtaaa gactgggacg
    23281 cgctggtaaa acgcgccaac gccgggaaac tcgatggcgt gaatgtgtta ttacgcccgg
    23341 tgagtgcgga atcgctggat aacctggtgg ccacctctac cgcgccgttt atcacgcatg
    23401 aaacggcgcg agcggcacaa tcactaaaca gtccggcccc cggcggattc ctgattgtca
    23461 gcgacgaagg cagcgatttt gttgatcagc cctggccttc ggcatcactt tacgactatc
    23521 cgccgcaaga acagtggaac gctttccaga aactggcaca aatgctgatg catacgccgt
    23581 ttaacgccga aggtatcgtc acaaaaatct tcactgacgc caatggtacg cagcatattg
    23641 gccttcatcc gatcccggat cgttccggcc tgtggcgcta tctcagcacc acgctgctgc
    23701 tactgacgat gctgggtagc gccatttata atggcgtaca ggcctggcgt cgttaccagc
    23761 gccatcgtac tcgcatgatg aagattcagg cctattatga aagttgcctg aacccgcaac
    23821 tgatcacccc ttcagaaagc cttatcgaat aacacgtttg cgcggcaggt tatgctaccc
    23881 tgtcgcgcaa attgcttcac tctggagatt tccctcatgc atatcaacat tgcctggcag
    23941 gacgtagata ccgttctgct ggatatggac ggcacgttgc tcgacctcgc cttcgataac
    24001 tatttctggc aaaagctggt gcctgaaaca tggggcgcga aaaacggagt tacgccacag
    24061 gaagcgatgg aatatatgcg ccagcaatat cacgacgtac agcatacgct aaactggtac
    24121 tgtcttgatt actggagtga gcaactgggt ctggatatct gtgcgatgac caccgagatg
    24181 ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc ttgaggcact gaaagccagc
    24241 ggtaaacagc gaattttgct caccaatgcg catccgcaca acctggcggt aaaacttgag
    24301 cataccggtc tggacgcaca ccttgattta ttactttcca cccacacatt tggttatccg
    24361 aaagaggatc agcggttatg gcatgcggtg gccgaagcta cgggtcttaa agctgaaaga
    24421 acgctgttta ttgatgacag cgaagcgatt ctcgatgctg ccgcgcaatt tggtattcgt
    24481 tactgcctcg gtgtgactaa tcctgactcc gggattgccg agaaacagta tcaacgccat
    24541 ccgtcactga atgactaccg ccgcctgatc ccctcgctaa tgtgaaggag atgccatgaa
    24601 agaaaaacct gctgttgagg ttcgactgga taaatggcta tgggctgccc gtttttataa
    24661 aacccgcgcg ctggcccgtg aaatgattga aggcggtaag gtgcattaca acgggcagcg
    24721 cagcaagcca agcaaaatcg tcgagctgaa tgccacgctc actctgcgcc agggaaatga
    24781 cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt cgccccgcca gcgaggcagc
    24841 cttgctgtat gaagagactg cggaaagtgt agagaaacgc gaaaaaatgg cgctggcacg
    24901 taaacttaat gccttaacca tgccgcatcc ggaccgacgc ccggacaaaa aagagcgccg
    24961 cgacctgtta cgatttaaac acggcgacag tgaataactg tcacctgcaa gagagatgat
    25021 tatgccgcaa catgaccaat tacatcgcta tctgtttgaa aactttgccg ttcgcggcga
    25081 actggtaacc gtttcggaaa ccctgcaaca gatccttgag aatcacgatt atccgcagcc
    25141 cgttaagaac gtgctggcag aactgctggt tgcgaccagc ctgttaaccg ctacgttgaa
    25201 gtttgatggt gatatcaccg tacagctgca aggcgacggt ccgatgaatc tggcggttat
    25261 taacggtaac aacaaccagc agatgcgcgg tgtggcgcgc gtgcagggcg aaattccaga
    25321 aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg gtgatcacca tcaccccgag
    25381 cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt gataccctgg cggcctgcct
    25441 ggaagattac tttatgcgtt ctgaacagct gccgacgcgc ctgtttattc gcaccggcga
    25501 cgtagacggc aaaccggctg caggcggaat gttgttgcag gtaatgcctg cgcaaaatgc
    25561 ccagcaggac gactttgacc acctggcgac gctaaccgaa accatcaaaa ccgaagaact
    25621 gctgacttta ccggcaaacg aagtgttgtg gcgtttgtat cacgaagaag aggtgacggt
    25681 ttacgatccg caggatgtgg agttcaaatg cacctgctcg cgtgaacgtt gcgccgatgc
    25741 gctgaaaacg ctgcctgatg aagaagtcga tagcatcctg gcggaagatg gcgaaattga
    25801 catgcattgt gattactgcg gtaaccacta tctgttcaat gcgatggata ttgccgagat
    25861 ccgcaacaac gcgtctccgg cagatccgca agttcattaa tgagtatgtc cggcagagag
    25921 gactctgccg gattttacga ctaatacacc tgatgcgctt tgtcactcat tcagattcac
    25981 atccagcctt tcaccatcca ctacaagatc gcgccacgct ttaggcctcg tccccagctt
    26041 tccatccttc gcagcagcga gtaatttttc tttcgttatt tggcgcggaa gtaaactcat
    26101 atcaagcgcg tcccattctt ttctggctga gtcaaagcca tagacaatga ctctgtcatc
    26161 attaaaagca aacaggatcc gctccgcctg accatcacta ttcagatctt gctcgaccag
    26221 tacgcaggcg tctttttcaa tacaggtcat cacgttatag cgttctttga ttaatgccga
    26281 ccagaatgcc acatccggtt tgccagaacc tggtgcaatt aacacatttt cggctaatga
    26341 tttttccgac accaccttct gaagactttg ctccccatct aacgccatca acagatcccg
    26401 cgcacgtttc gggtctttca taaacccggc atcgcttttc agcgactcaa gcgccgcacg
    26461 tccatagcga ccgctgtgtt cgagcatgta gatagttacc tggtctggcg tgtttttacc
    26521 gctctgataa cgcgccatat gactgttcac gctaatgcgc atactgtcca gcaccggcga
    26581 attaagaagc accagtatca ccaacaccaa taccgaaacc gcaaggttca ctttgccttg
    26641 aagaacaagg ggattttgcc ctttacgcca gacaatgctg acaaaatacc ccagcgacca
    26701 caccagtaac accagcaccg ccagcgcgcc ctgcaagcgg tcgacagtcc agccgtactg
    26761 agcgacccgc agccataacg cccaggcggc gacgaacaca tacagcggag caaccagcaa
    26821 agcggtttta atcaggcaac gcaatggccc cgtccaggga agtgacgctt tttgcggatc
    26881 gcggacaata gccattaaga tcaattgcaa aaaggccagc gttaacagca acccagcggc
    26941 ggagatgtgg cgagaaatcg cgctcaggcc cgtaaacggc agggtgataa taaacatcag
    27001 ggttagcaat gatactaacg gcagcaaccc cgtggcgatt aacgtgaaca acttttgaat
    27061 agagtcgatt aaacgtgact gtgttcgcgc gaggatcacc gccagcgcgg tagccagacc
    27121 taacgtgaga taaataaacc agtcggttgc aaaaaagagc gttttgaaaa acgtgatgcc
    27181 aacaagttta aacaactcac tccacagtaa aagcaccagc cacgtcaggc cattggcgag
    27241 aaaaatcacc agtaatataa gtaagttatg ccatacagac tggtaaaagt agcgatagcg
    27301 gctactgtca ttgcggatgc gaaggctttg ttgtatccac ggtagcaaca acattgccat
    27361 cagcagcaga tagcagccaa aatcccaaag cgccttttca gctctccagg gggtcatgcc
    27421 atcagtttgc cacttcagcc agccgctcat tcccagcgtg gcaataaaca ccagcgccag
    27481 ccatccccaa aggcgattct gtttaaagga gatcacggag aagagtaaaa cggatgaaaa
    27541 cgcgacggtc gcaggcacgc cataaacaat ccaactgaga ttttttccgg caagccaggc
    27601 gatcagcagg tagcaaacca acccctgaag taatccggta gcaatcatcc cccagcgtgt
    27661 tgccggtgaa agttcaacgt tatccatgtt gtttatccag aatcaaaagg tgggttaatt
    27721 atcgcatccg ggcagtagta ttttgctttt ttcaaaaaat aatcaaaaaa agttagcgtg
    27781 gtgaatcgat actttaccga ttgaatttgc atcaatttca ttcatgaatg cgattccact
    27841 cacaatattc ccgccatata aaccaagatt taaccttttg agaacatttt ccacacctaa
    27901 aatgctattt ctgcgataat agcaaccgtt tcgtgacagg aatcacggag ttttttgtca
    27961 aatatgaatt tctccagata cgtaaatcta tgagccttgt cgcggttaac acccccaaaa
    28021 agactttact attcaggcaa tacatattgg ctaaggagca gtgaaatgcg cgttaacaat
    28081 ggtttgaccc cgcaagaact cgaggcttat ggtatcagtg acgtacatga tatcgtttac
    28141 aacccaagct acgacctgct gtatcaggaa gagctcgatc cgagcctgac aggttatgag
    28201 cgcggggtgt taactaatct gggtgccgtt gccgtcgata ccgggatctt caccggtcgt
    28261 tcaccgaaag ataagtatat cgttcgtgac gataccactc gcgatacttt ctggtgggca
    28321 gacaaaggca aaggtaagaa cgacaacaaa cctctctctc cggaaacctg gcagcatctg
    28381 aaaggcctgg tgaccaaaca gctttccggc aaacgtctgt tcgttgtcga cgccttctgt
    28441 ggtgcgaacc cggatactcg tctttccgtc cgtttcatca ccgaagtggc ctggcaggcg
    28501 cattttgtca aaaacatgtt tattcgcccg agcgatgaag agctggcagg tttcaaacca
    28561 gactttatcg ttatgaacgg cgcgaagtgc actaacccgc agtggaaaga acagggtctg
    28621 aactccgaaa acttcgtggc gtttaacctg accgagcgca tgcagctgat tggcggcacc
    28681 tggtacggcg gcgaaatgaa gaaagggatg ttctcgatga tgaactacct gctgccgctg
    28741 aaaggtatcg cttctatgca ctgctccgcc aacgttggcg agaaaggcga tgttgcggtg
    28801 ttcttcggcc tttccggcac cggtaaaacc accctttcca ccgacccgaa acgccgcctg
    28861 attggcgatg acgagcacgg ctgggatgat gacggtgtgt ttaacttcga aggcggctgc
    28921 tacgcgaaaa ctatcaagct gtcgaaagaa gcggaacctg aaatctacaa cgctatccgt
    28981 cgcgatgcgc tgctggaaaa cgtcaccgtg cgtgaagatg gcacaatcga ctttgatgat
    29041 ggttcaaaaa ccgagaacac ccgcgtttct tatccgatct atcacatcga taacatcgtt
    29101 aagccggttt ccaaagcggg ccacgcaacg aaagttatct tcctgacagc agacgccttt
    29161 ggtgtgctgc cgccggtttc tcgtctgact gccgatcaaa cccagtacca cttcctctct
    29221 ggcttcaccg ccaaactggc cgggactgag cgtggcatca ccgaaccgac gccaaccttc
    29281 tccgcctgct tcggcgcggc gttcctgtcg ctgcacccaa ctcagtacgc agaagtgctg
    29341 gtaaaacgta tgcaggcggc gggtgcgcag gcgtatctgg ttaacaccgg ctggaacggt
    29401 actggcaaac gtatctccat taaagatacc cgcgccatta tcgacgccat cctcaacggt
    29461 tcgctggata acgcagaaac tttcactctg ccgatgttta acctggcgat cccaaccgaa
    29521 ctgccgggcg tagacacgaa gatcctcgat ccgcgtaaca cctacgcttc tccggaacag
    29581 tggcaggaaa aagccgaaac gctggcgaaa ctgtttatcg acaacttcga taaatacacc
    29641 gacacccctg cgggtgccgc gctggtagcg gctggtccga agctgtaatg atttgaagct
    29701 ggagaatatc tatccagtat cttatagaaa gcaaaacggg aggcaccttc gcctcccgtt
    29761 ttatttaccc ttcttttgtc gcaccctgcg cccgcgttac cggcacaggc agccaggcgc
    29821 gaatggaaag cccgccccgt tcgctggtgc caagctccag catcccgtta tggttatcca
    29881 cgatacgctg cacaatcgcc agccctaatc ccgtgccgct aatagtgcgc gcactgtcgc
    29941 cacgaacaaa cggctggaac aggtgcttac gttgttccgg cgcaataccc ggaccgtcat
    30001 cttctacctg gaaccaagcg cgattcggct ccgtgccgct gctgactttg atccagccgt
    30061 tgccatagcg ggcggcgttc accaccatat tcgctaccgc gcgtttgatc gacagcgggt
    30121 gcattttcac ctcaatgcta ccggggtaaa gcgcagtttc aatttcccgc tcatagccac
    30181 tttcggcagc aataacctca ccgagtactg cgttgagatc cgccatttcc atcggcatct
    30241 cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac tcttcgatat
    30301 ctttattgat cgattctgcc aggtagccat cctgctcgct catcatctcg gttgccaggc
    30361 gaatacgcgt cagaggcgtg cgcagatcat gacttacccc tgccatcagc agcgtgcggt
    30421 catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcgcgggta acggaacgca
    30481 cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttaccc acctgcaagg
    30541 ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc cacgccccgc
    30601 ctatcgccaa tagcataatc gccaacgtat aacggaacag cggggagaaa tcgccctgat
    30661 gaatttcggt tagcggcacg cgtacccaga tattgggcga caaccaggtt ttcagccaga
    30721 cgacaggcga acttttgtta acctcgacgc gaacttccgt cggaccgccc agttgctgcg
    30781 ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc tcttcggcag
    30841 cttcgttgga atagagagag atccccagct cacggtagat ctcccgacgg aaagcgggag
    30901 gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac atacgcactt
    30961 cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag ttcagcacca
    31021 ccagataagt cgtcaccagg ctgacgaaca gcaaggtgac gatgagcaat aacgtacggg
    31081 caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagaaccgt ccggtacaaa
    31141 gacgtagccc agaccccaga cggtctgaat gtaacgcgga tgcgccggat cttcttccac
    31201 catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg aatattcacg
    31261 accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat ggctgaccag
    31321 cgccttaagt accgcaaact caccactggt gagcggcatc ggctcgtctt cgcggaacat
    31381 ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct cttcctgtga
    31441 cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatgc gggccagcaa
    31501 ttcacgcggg ttaaacggtt ttggaatgta atcgtcagcg ccaatctcta ggccaacgat
    31561 acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcggat tgctctggct
    31621 acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacatcaaat ccagtaccat
    31681 aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag cgacgcttcg
    31741 aacctggaag ccttgttcgg tgagataacg ttccagcagc gcgcgcaggc gcatgtcgtc
    31801 atcaaccacc agaatcttgt agttctcttg cattgtttat actcccaaag gttcgcaaca
    31861 atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat ctggtatgaa
    31921 tttcagccta aattgttaca aagcatatta aacagcagct taagtataca atttattcgg
    31981 cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc attatcaaac
    32041 agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg tatgaaaaac
    32101 tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca aagaaagtga
    32161 cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat aataaaaagc
    32221 gcctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcatggaa aatctcaaaa
    32281 tcgtcgatta ctccccgcag caggaaggca aagtcttttt tggtgcgtgg gtggagattg
    32341 aaaacgacga tggtgtgact caccgtttcc gtattgtcgg ctacgatgaa atttttggcc
    32401 gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa aaagaagtcg
    32461 gcgatctggc ggtggtgaat acccctgccg gggaagcgag ctggtatgtt aatgctatcg
    32521 agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca tttttgccag
    32581 ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa accatgatga
    32641 atgattcgtt ctgccgcatt attgcgggtg aaattcaggc acgcccggaa caggttgacg
    32701 ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt tatcgtaagg
    32761 aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt ctgagctatc
    32821 tgcgcgagct ggaagagaga cgtcaggcga tcctcaagtc tatttccgag caaggcaaac
    32881 tcaccgatga tctggcgaat gccatcaacg ccaccctaag caaaaccgaa ctcgaagacc
    32941 tctacctgcc ctacaaacct aaacgccgca cccgtgggca aatcgccatt gaagcagggc
    33001 ttgagccgtt ggccgatctg ctgtggagcg atccgtcaca cacgccagaa gtcgccgctg
    33061 cacaatatat tgatgccgat aaaggcgtgg cagataccaa agccgcgctg gacggcgcgc
    33121 gctatatcct gatggaacgg tttgccgaag atgccgcgct gctggcgaaa gtgcgtgatt
    33181 atctgtggaa gaacgcgcat ttggtctcaa ccgtggtgaa tggtaaagaa gaggaagggg
    33241 cgaaattccg cgactatttc gatcatcacg aaccgctgtc cacggtgcct tctcaccgcg
    33301 cgctggcgat gttccgtggt cgtaacgaag gcatacttca gctttcgctg aatgccgatc
    33361 cgcagttcga agagccgccg aaagagagct attgcgagca aatcatcatg gatcaccttg
    33421 gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aggtgtggtg agctggacgt
    33481 ggcgcatcaa ggtgttgatg catctggaaa ctgaactgat gggcaccgtg cgcgaacgcg
    33541 cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg atggcggccc
    33601 ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg gtaaaagtgg
    33661 cggtagtcga tgcgacaggt aaactggtgg cggccgatac catttatccg cataccgggc
    33721 aggccgcaaa agcagcgatg accgttgctg cgctgtgtga aaagcataac gttgaactgg
    33781 tggcgattgg caacggtaca gcttcccgcg aaaccgaacg tttctatctc gacgtgcaga
    33841 agcagttccc gaaagtgacc gcgcagaaag tgatcgtcag cgaagctggc gcgtcggttt
    33901 actcggcttc cgagctggca gcacaggaat tcccggatct cgacgtttcg ctgcgtggcg
    33961 cggtgtctat cgcccgtcgt ttgcaggatc cgctggcgga gctggtgaaa atcgatccga
    34021 aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcagctg gcccgcaaac
    34081 tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac accgcttctg
    34141 ttcctctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac atcgtggcct
    34201 ggcgcgatga gaacggccag ttccagaacc gtcagcaact gttaaaagtg agccgtctgg
    34261 ggccgaaagc cttcgagcag tgcgcgggct tcttgcgcat taaccacggt gataacccgc
    34321 tggacgcttc taccgttcac ccggaagcct atccagtggt ggaacgcatt ctggcagcaa
    34381 cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac ctgaaagcat
    34441 ctgactttac tgatgagaaa ttcggtgtgc cgacggtaac cgacatcatc aaagagctgg
    34501 aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc gatggcgtcg
    34561 agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg tgcggtgacc aacgtcacca
    34621 actttggcgc gtttgtcgat attggtgtgc atcaggacgg cctggttcac atctcttcat
    34681 tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac attgtgaagg
    34741 tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgaccatg cgtctggacg
    34801 agcagcctgg cgaaaccaac gcccgtcgcg gtggcggtaa cgatcgcccg caaaacaacc
    34861 gcccggcagc caaaccgcgt gggcgtgaag cgcagcctgc cggtaatagc gcgatgatgg
    34921 atgcgctggc ggcggcaatg ggcaaaaaac gttaaacgcc cggactggcc cctaaacctg
    34981 cgcgattagg cgttcacgcc gcatctggca atgaacgcct tgcctgacat aaaagtgccg
    35041 gagaatatct ccggtctttt tattccacaa gcaaactcat aatagattcc gttaatattt
    35101 atcacttcat taacaactga aaccttaatt aaacattaac cagccctaat aattcgtcat
    35161 gcgaattata ttttcgctgc gatataacct tgagccatat caacattgag tcagattatt
    35221 attcaaacca acattcacac acattttaag tattgctgat agaaaccatt ctcattatca
    35281 ttgtactgtt gattatttaa tctctccttc gttggcaaat catctggtct catgtcgctg
    35341 tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac ctatgcaata
    35401 cactccagat actgcgtgga aaattactgg cttttcccgt gaaatcagcc cggcatatcg
    35461 ccaaaaactg ctttctcttg gcatgttacc tggctcctct tttaatgtgg tgcgcgtcgc
    35521 tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat tacgcaaaaa
    35581 agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata acaacaatga
    35641 aaaaattaac cattggctta attggtaatc caaattctgg caagacaacg ttatttaacc
    35701 agctcactgg ctcacgtcag cgtgtaggta actgggctgg cgttaccgtc gaacgtaaag
    35761 aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc ggcacctatt
    35821 ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt cactacattt
    35881 tgagtggcga cgcagacctg ctgattaacg tggtggatgc gtctaacctt gagcgtaacc
    35941 tgtacctgac gctgcaactg ttggaactcg gcattccatg catcgtggcg ctgaacatgc
    36001 tcgacattgc cgagaagcaa aatattcgca ttgaaattga tgcgctgtcg gcgcgtcttg
    36061 gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg ctcaagctgg
    36121 cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgct cagccgctgc
    36181 tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg aaacaacgtc
    36241 gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac gccggtgaag
    36301 cgtcgcagca tctggatgcc gccctcgccc gtctgcgtaa tgagatggac gatccggcgc
    36361 tgcacattgc cgatgcgcgt taccagtgca tttctgccat ctgtgatgtg gtaagcaaca
    36421 ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg ctcaaccgtt
    36481 tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcttgctg gctatcaaca
    36541 tcggcggggc gttacagccg ctgtttgacg tcggctccgt agcactcttt gtgcatggta
    36601 ttcagtggat tggctacacg ctccacttcc cggactggct gactatcttc ctcgcccaag
    36661 gcctgggcgg tggtattaac accgtgctgc cactggtgcc gcagatcggc atgatgtacc
    36721 tgttcctctc cttccttgag gactccggct atatggcgcg tgcggcgttt gtgatggacc
    36781 gtctgatgca ggcgctgggc ttgccgggga aatcctttgt accgctgatc gttggtttcg
    36841 gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt gaacgtctga
    36901 tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc ttcgcagtat
    36961 tcgcggctgc cttcttcggg cagaacggtg cgctggcggt cttctcgctg tatatgctgg
    37021 gtatcgtaat ggcggtgctg actggcctga tgctcaagta caccatcatg cgcggtgaag
    37081 cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa agcctgatta
    37141 tccagacctg gcagcgtctg aaaggcttcg ttctgcgtgc tggtaaagtg attatcatcg
    37201 tcagcatttt cctgagcgcc ttcaacagct tctcactgag cgggaaaatc gtcgataaca
    37261 tcaacgactc ggcgctggct tccgtcagcc gggtgatcac cccggtcttc aagccgattg
    37321 gcgtacatga agataactgg caggcaacgg ttggcctgtt tacaggtgcc atggcgaaag
    37381 aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag gacgaagagt
    37441 tcaatccggc ggaatttaac ctcggtgaag agctgttcag tgcggtagat gaaacctggc
    37501 agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa gccagcaaag
    37561 gcgacggcga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc ggtagcgcag
    37621 ctgccgcata cagctacctg attttcgtcc tgctgtacgt accgtgcatc tcggtgatgg
    37681 gggctatcgc gcgtgaatca agccgtggct ggatgggctt ctccatcctg tgggggctga
    37741 atattgctta ctcactggca acattgttct atcaggtcgc cagctacagc cagcatccaa
    37801 cttacagcct ggtgtgcatt ctggcagtta tcctgtttaa catcgtggtt atcggtctgc
    37861 tgcgccgcgc gcgtagccgg gtagatgtcg aactgctggc aacgcgcaaa tcggtaagca
    37921 gttgctgtgc ggccagcacc actggcgatt gccactaagg acacgacaat ggcttcgctc
    37981 attcaagtgc gtgatttact ggcattacgg ggccgtatgg aagcggccca gattagccag
    38041 acactgaaca ctccgcagcc aatgattaac gccatgctga aacagctaga aagtatggga
    38101 aaagcagtgc ggattcagga agaacctgac ggctgtcttt ccggcagttg taaaagctgc
    38161 ccggaaggaa aagcctgtct gcacgaatgg tgggcgttgc gttaaccttg tttcactgcc
    38221 tgttttgaaa gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa
    38281 aatgcaaccc ttgcagcgcg acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact
    38341 cgctaatgca caggtgtgga gtggcgcgta gagtcgcggc attcaaataa caggtgaagg
    38401 aacgccatga gcaaaaagca gagttccacc ccacacgatg cgttacgcca ttgccgcaat
    38461 gcaaaatcac cttgatgccg gatacaaaac attgccgatg gtggtgccac tgttgtttta
    38521 tcacggtatt gaaagctcct atccctattc gctgtgttgg ctggattgtt tcgccgctcc
    38581 caacctggca aggcagcttt atgcctctgc atttccgctg attgatatca ccctcatgcc
    38641 tgatgatgaa atcatgctgc accgacgtat ggcgctactg gagttaattc aaaaacatat
    38701 tcgtcaacgc gacctgatgg ggctggtaga gcaaatggcc tgcttattaa gtagtggata
    38761 cgctaatggc agacaaatca aagggctgtt taattacata ctgcaaaccg gcgacgcggt
    38821 acgttttaac gattttatcg atggtgtggc cgaacgttca ccaaaacaca aggagagttt
    38881 aatgactatt gcggaaagat tgcggcagga aggggaacaa tccaaagcct tgcatatagc
    38941 aaaaataatg cttgaatccg gagtccctct tgcagacatc atgcgcttta ccggggtgtc
    39001 agaagaagag ttggctgcgg cgagccagta aagttctgtc ccgccatttc gaaagccacc
    39061 tacaccctct gcttcaacgc caccagcaga tgacaaaact cgtccggatg tgaaataaac
    39121 ggcgcatggg ccgctttggc gaagatatat gattcgctgt gagtccaaag tttatccagc
    39181 atcggcacca ctttgcgcgg caccagaccg tcgagatagc catacaatcg caaaaacggc
    39241 atgggcacgt tttgcagcgg ctgacggaga tcgaccgttt tcaggatttc cagcccgcca
    39301 ttaagcacgt caacctccgg catcggtaac gccagaacgg ttttcttcaa cgcccgcgca
    39361 tcctggcgcg ccgtttcagt acccatggtt tgtaacgcca ggaaccgctc cactgtacgc
    39421 tgaaaatcat cactgagttg ctgctgaaat cccgccagca cgtccggttt tatccccggc
    39481 cactcgtcgc gagcactaaa acaaggtgac gacgccacgg tgaccagcgc cagaacccgc
    39541 tcgggatggg ttaacgcaat ctggcttgcc accagcccgc ccagactcca gcctaaccaa
    39601 atggctttat ctggtgcctg tcgcagcacg acttcggcca tatcagcaag tgatagcgca
    39661 ccaaatcccc ggctacgccc gaagccgggc aggtcaacaa ggtgcagcgt aaaatgcgag
    39721 ctaagttcct cgtcaatgca acgccacact tcggcattca gtccccatcc gtgcagcagc
    39781 acaagatgaa cattcccctg acctttggtc tgccaccaga tgttattcat ccgctattgt
    39841 tctcttttta cttacaagga tgaacatatg ctaacagtac cgggattatg ctggctatgc
    39901 cgaatgccac tggcgttagg tcattggggg atttgttcgg tctgctctcg cgccgcccgt
    39961 acagataaaa cgttatgccc acaatgtgga ttacccgcca cacactctca tcttccctgc
    40021 ggtcgctgcc tgcaaaaacc gctgccctgg caaagactgg tcacggtcgc cgactatgcg
    40081 ccgccattaa gtccgctcat tcaccagctt aagttttccc ggcgcagtga aatcgccagc
    40141 gccctgtcac gtctgttact attggaagtc ttacacgctc gtcgcaccac cggcttgcaa
    40201 ctgcctgatc gcatcatcag cgttccgtta tggcagcgcc gtcactggcg tcggggattt
    40261 aatcagagcg atttgctgtg tcagccgtta tcacgctggc tacactgcca atgggatagc
    40321 gaagccgtca cacgaacacg ggccaccgcg acccagcatt ttcttaacgc ccggctacgc
    40381 aaacacaacc tgaaaaatgc ctttcgcctt gaattgcctg tgcaaggtcg ccatatggtg
    40441 attgtggatg atgtcgttac caccggaagt accgtcgcag agattgcgca gttgctttta
    40501 cgcaatggtg cggcgactgt ccaggtctgg tgcctttgtc gaaccttgta gagcctcgat
    40561 gatgggcgta ttataaccaa ctaaaatagt caactattag gccattacta tgatccgtat
    40621 ttccgatgct gcacaagcgc actttgccaa actgctggca aaccaggaag aagggacaca
    40681 aatccgcgta tttgtgatta accctggcac gcctaacgct gaatgtggcg tttcttattg
    40741 tccgccggac gctgtggaag ccaccgacac tgccctgaaa tttgacctgc tgaccgcgta
    40801 tgttgatgag ttaagcgctc cttacctgga agatgcagag atcgattttg ttaccgacca
    40861 gttgggttcg cagctgacgc tgaaagcgcc taacgccaaa atgcgtaaag tggctgacga
    40921 tgcgccgctg atggagcgcg tggagtatat gctgcaatca cagatcaacc cacagcttgc
    40981 tggtcacggt ggtcgcgttt cgctgatgga aatcaccgaa gacggttacg ccattttgca
    41041 atttggcggt ggctgtaacg gttgttccat ggtcgacgtc accctgaaag aagggatcga
    41101 aaagcagttg ctgaacgaat tcccggagct gaaaggtgta cgcgatctca ccgaacacca
    41161 acgcggcgag cactcttact actaagttct ctcccctttt gtcgattgcc cgatgtgacg
    41221 ctaaagcgtc ttatcgggcc tgtacgttgc acgagagtct gacgttagcg cagaattacc
    41281 tcagcaaatc caatctcttc gtttccccac gataatatga ccaacctctc ataatttaaa
    41341 tttaccccgc tctggtgatt ctcaagcacg taatgttacc cgtatcattc ccatgggcac
    41401 caaacacact cataacatct gactacacta actcgtttta ggggtcatca gccgtggtgc
    41461 cgtaacatct ctgttcccag ttgggacaac caggattttc cgtcagaaac ctgacgttac
    41521 ccataacaaa ttaaaggcca ggtaaatcat gccattagtc attgttgcta tcggtgtaat
    41581 cttgttatta ctcctgatga tccgcttcaa aatgaacggc ttcatcgctc tcgtcctcgt
    41641 ggcgcttgct gttggattaa tgcaagggat gccgctggat aaagttattg gctccatcaa
    41701 agctggtgtc ggcggaacgc tcggtagcct tgccctgatc atgggttttg gtgcgatgct
    41761 gggcaaaatg ctggcagact gcggtggtgc acaacgtatc gctaccacac tgattgccaa
    41821 atttggtaaa aaacacatcc agtgggcggt ggtactgacc ggttttaccg ttggttttgc
    41881 cctgttctat gaagtgggtt tcgtgctgat gctgccgctg gtgtttacca tcgcggcttc
    41941 cgcgaatatt ccactgctgt atgttggtgt accaatggcg gctgcactgt ctgtgaccca
    42001 cggcttcctg ccaccgcatc cgggtccgac tgcaattgcc accattttca atgccgatat
    42061 gggtaaaact ctgctgtacg gtactattct ggcaatcccg accgtgattc tcgccggtcc
    42121 ggtttacgct cgcgtgctga aaggtatcga taagccgatc ccggaaggtc tgtacagcgc
    42181 gaaaaccttc agcgaagaag agatgccgag ctttggcgtc agcgtctgga cctctctggt
    42241 gccggtggtt ctgatggcga tgcgtgcgat tgccgaaatg atcctgccga aaggtcacgc
    42301 tttcctgccg gtggcggagt tcctcggtga cccggtaatg gccacgctga ttgccgtgct
    42361 gattgcgatg ttcacctttg gtctgaaccg tggtcgttca atggatcaga ttaacgacac
    42421 gctggtttct tccatcaaaa tcatagcgat gatgctgttg attatcggtg gtggcggtgc
    42481 gttcaagcag gtgctggtag acagcggcgt ggacaaatac attgcttcca tgatgcacga
    42541 aaccaacatt tctccgctgc tgatggcctg gtcgattgct gccgtactgc gtatcgcgct
    42601 gggttccgca accgtcgcgg caatcactgc gggtggtatc gcggcaccgc tgattgcaac
    42661 gacgggtgtt agcccggagc tgatggttat tgcggttggt tccggtagtg tgattttctc
    42721 tcacgtgaac gatccgggct tctggctgtt caaagagtac tttaacctga ctatcggcga
    42781 gaccatcaaa tcctggtcga tgctggaaac cattatctcg gtgtgcggtc tggtaggctg
    42841 tctgctgctg aatatggtga tttgataata ctgatggccg gatgcggcgt gaacgcctta
    42901 tccggcctac gattcggcat taattagtag gccggataag acgcgtcaag cgtcgcatcc
    42961 ggcatcaacc gcactctacg tcttcttcgc tgccgctctg cgccgtctgt ccaaatcctt
    43021 cagcaacttg ttcacaccat catcggcaaa catcgactca agcgttgcgg aaagtttacg
    43081 tcgccagttt ttatactggt aactggtgcc gggaatattt accggatctg ccatatccag
    43141 ccagtcttcc ggctgtagcc ctaacagggc gctgttgcta tcggcgatgt agcgctgtaa
    43201 gccacgattc agcgtcggtg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
    43261 caggcaacca tatttatgca gagcatccag cagcccttgc ttcgccagtt cgcgatcctg
    43321 atacagaccg cgcagtacca cttcatccgg atacagcccc agcgttttgc ccagcgttaa
    43381 atccccactc tcccagtagc cgcgtagcgt aggcaggtcg tgtgtcgccg caaccgccat
    43441 cgactgctct gggtacgctt tcggtgcgcg gaatgtcttc tcgtggtcgt tttcgaaata
    43501 gagcactttg taggagtaca caccgctgct gcgcagctta ccgacaatct ctaccggtac
    43561 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
    43621 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
    43681 cggtatccac cacaaacgca gcatcgacat cacgtgatca attcgtaatg cgccgcagtt
    43741 ttgcatattg gaacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
    43801 cgggtccatt ggcggcaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
    43861 gccaaccgac gctttcaggc aatacagttc acggtcgcac caggtttctg ccccaccttc
    43921 tgctacacca acggccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
    43981 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
    44041 gtcatcacga tgttcttcgc agaactggcg cacttctggt gaatccacgt tctgatacat
    44101 ctctggccat gctggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
    44161 cgcatcaaag gctgcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
    44221 cgcggtcatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
    44281 catttttagg gtggtaaccg tggagtaatc aacccagtcg gcatcccgcg cctgttgcag
    44341 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
    44401 ttcaactgcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
    44461 cgggctggca ctttccggat ttgccagata gagcgcatga atcgggttca gaccaataaa
    44521 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
    44581 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
    44641 tttattcagc aacgcctgcg gttcgtaaca gcgtttcggg gcgacaatca cccggcaatg
    44701 cgcgcgctgg tcgtcctggg taagcgtcag cgtgtgataa ccttccggca gcttcgtcgg
    44761 tagattgaac gctttgcccc ccatcacatg gcctttgtac tgctttcctt cttcggtggt
    44821 cagcagccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
    44881 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
    44941 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
    45001 gatgtaattg gggctgatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
    45061 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
    45121 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
    45181 gtcgaggtac agcacatcca cctgcttttg tgcctctaca taagcggcga aatctgccat
    45241 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
    45301 cttatcgccg tcgctgtatt taccgctttc cagatctttc aatactgcgt ccagcacctt
    45361 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaattgcct tcacttgttc
    45421 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcagcga tttcaacgtt
    45481 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
    45541 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
    45601 tttttccgcc gccgaaacac aataatccgg caagaacacc accttcaact tgtcgccaac
    45661 cagcggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tatttttcgc
    45721 caggtagtag cccggtgccg ctttcgcgcc aaagaggaag acgcgcggta cgcggtcaga
    45781 ctgcgggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcagatgctg
    45841 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
    45901 aataccggta cgaactttca caaactctgc cagacggact ttattcgcct gcttgatctc
    45961 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagttgatc
    46021 gagatcgtta gcccactctt ttttcagtga tttatccaac agagccgcca gtgccgggtt
    46081 gcactgtttg atccagcgac gtggggtaat accgttggtg acattatgga atttgttcgg
    46141 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatctgagt gcagcgccgc
    46201 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttgtc
    46261 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
    46321 cgttttaaag cgagtattaa tttcgttaat aatctgcatg tggcgcggca gtaagccttt
    46381 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
    46441 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcgatcag
    46501 cacgcgcagc agttctggaa tggcgatagt tgggtgggta tcgttcagct gaataacttc
    46561 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
    46621 taccgagcag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
    46681 gttgtcgttt ggatagagaa ctttggtcag tttttccgcg ttgatgccct gctgttcggc
    46741 acgcaggaaa tcaccatcgt taaatttagt cagatcaaac ggatgcgcat gcgtcgcctg
    46801 ccacagacgc agtggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
    46861 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcgtcactt taccgccaat
    46921 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac cgcgatgcca
    46981 gtcatccggc gcctcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
    47041 atagttcaga ccgtaacccg tcgcagactg accaacagtt gccattgagt cgaggaagca
    47101 cgccgccaga cgtcccagac caccgttccc cagcgccggg tcgatctctt cttccagcaa
    47161 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
    47221 attcaacagg ttgttgcccg tcaggcgacc aattaaaaac tccattgaga tgtagttaac
    47281 atgtcgctga ttcgccactg gcttggcgaa tggctgagca cgcagcattt cggccagtgc
    47341 ttcgctcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
    47401 acgctgccac tgacgtgaaa gcgcttcctg aaattgctta tcgttaaaaa taggttgtga
    47461 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
    47521 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
    47581 ggaaatgtga tctcgaccac ttaaagctag tgcaatccac aggattagca gcaaatcaat
    47641 gccataccgc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
    47701 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
    47761 caaatgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
    47821 tcatcagaaa tgaagaagaa acccttactt aaatgaaact gatgaacata aggaaaatac
    47881 cgtacaaccc agtattcacg ctggatcagc gtcgttttag gtgagttgtt aataaacatt
    47941 tggaattgtg acacagtgca aattcagaca cataaaaaaa cgtcatcgct tgcattagaa
    48001 aggtttctgg ccgaccttat aaccattaat tacgaagcgc aaaaaaaata atatttcctc
    48061 attttccaca gtgaagtgat taactatgct gattccgtca aaattaagtc gtccggttcg
    48121 actcgaccat accgtggttc gtgagcgcct gctggctaaa ctttccggcg cgaacaactt
    48181 ccggctggcg ctgatcacaa gtcctgcggg ctacggaaag accacgctca tttcccagtg
    48241 ggcggcaggc aaaaacgata tcggctggta ctcgctggat gaaggtgata accagcaaga
    48301 gcgtttcgcc agctatctca ttgccgccgt gcaacaggca accaacggtc actgcgcgat
    48361 atgtgagacg atggcgcaaa aacggcaata tgccagcctg acgtcactct tcgcccagct
    48421 tttcattgag ctggcggaat ggcatagccc actttatctg gtcatcgatg actatcatct
    48481 gatcactaat ccagtgatcc acgagtcaat gcgcttcttt attcgccatc aaccagaaaa
    48541 tctcaccctt gtggtgttgt cacgcaacct tccgcaactg ggcattgcca atctgcgtgt
    48601 tcgtgaccag ctgctggaaa ttggcagtca gcaactggca tttacccatc aggaagcgaa
    48661 acagtttttt gattgccgtc tgtcatcgcc aattgaagcc gcagaaagca gtcggatttg
    48721 tgatgatgtt tccggttggg cgacggcact gcagctaatc gccctctccg cccggcagaa
    48781 tactcactca gcccataagt cggcacgccg cctggcggga atcaatgcca gccatctttc
    48841 ggattatctg gtcgatgagg ttttggataa cgtcgatctc gcaacgcgcc attttctgtt
    48901 gaaaagcgcc attttgcgct caatgaacga tgcactcatc acccgtgtga ccggcgaaga
    48961 aaacgggcaa atgcgcctcg aagagattga gcgtcagggg ctgtttttac agcggatgga
    49021 tgataccggc gagtggtttt gctatcaccc actgtttggt aacttcttgc gccagcgctg
    49081 ccagtgggaa ctggcggcgg agctgccgga aatccaccgt gccgccgcag aaagctggat
    49141 ggcccaggga tttcccagcg aagcgattca tcatgcgctg gcggcaggcg atgcgctgat
    49201 gctgcgcgat attctgctta atcacgcctg gagtttgttc aaccatagcg aactgtcgct
    49261 gctggaagag tcgcttaaga ccctgccgtg ggacagtttg ctggaaaatc cgcagttggt
    49321 gttattgcag gcgtggctga tgcaaagcca gcatcgctac ggcgaagtta acaccctgct
    49381 ggcccgcgct gaacatgaaa tcaaggacat cagagaaggc accatgcacg cagaatttaa
    49441 cgctctgcgc gcccaggtgg cgattaacga tggtaacccg gatgaagcgg aacggctggc
    49501 aaaactggca ctggaagagc tgccgccggg ctggttctat agccgcattg tggcaacctc
    49561 ggtgctgggt gaagtgttgc actgcaaagg cgaattgacc cgctcactgg cgttaatgca
    49621 gcaaaccgaa cagatggcac gccagcacga tgtctggcac tacgctttgt ggagtttaat
    49681 ccagcaaagt gaaattctgt ttgcccaagg gttcctgcaa accgcgtggg aaacgcagga
    49741 aaaagcgttc cagctgatca acgagcagca tctggaacag ctgccaatgc atgagtttct
    49801 ggtacgcatc cgtgcgcagc tgttatgggc ctgggcacgg ctggatgaag ctgaagcgtc
    49861 ggcgcgcagc gggattgaag tcttgtcgtc ttatcagcca cagcaacagc ttcagtgcct
    49921 ggcaatgttg attcaatgct cgctggcccg tggtgattta gataacgccc gtagccagct
    49981 gaaccgtctg gaaaacctgc tggggaatgg caaatatcac agcgactgga tctctaacgc
    50041 caacaaagtc cgggtgattt actggcaaat gaccggcgat aaagccgccg ctgccaactg
    50101 gttgcgtcat acggctaaac cggagtttgc gaacaaccac ttcctgcaag gtcaatggcg
    50161 caacattgcc cgcgcacaaa tcttgctggg cgagtttgaa tctgcggaaa ttgtcctcga
    50221 agaactcaat gaaaatgccc gcagtctgcg gttgatgagc gatctcaacc gtaacctgtt
    50281 gctgcttaat caactgtact ggcaggccgg acgtaaaagt gacgcccagc gtgtgttgct
    50341 ggacgcatta aaactggcga atcgtaccgg atttatcagt cattttgtca tcgaaggcga
    50401 agcgatggcg caacaactgc gccagcttat tcaactcaat acgctgccgg agctggaaca
    50461 gcatcgcgca cagcgtattc tgcgagaaat caatcaacat catcggcata aattcgccca
    50521 tttcgatgag aatttcgttg aacgtctgct aaatcatccg gaagtgccag agctgatccg
    50581 cactagcccg ttgacccaac gtgaatggca ggtcctgggg ctgatttact ccggttacag
    50641 caatgagcaa attgcaggcg agctggaagt ggcggcaacc accatcaaaa cgcatatccg
    50701 taatctgtac caaaaactcg gtgtcgccca ccgtcaggcc gcggtacaac acgcgcagaa
    50761 actgctgaag atgatgggat atggggtatg agtttagccg gataatgcgc cagatccggc
    50821 ttacatcttt acatcattca atgctcaccc gcgttacgcc atctgtttct atcaaaccaa
    50881 accgcaccgg caagaaacgc tccaccacgg cgatattggt cagcagatgg catgagggat
    50941 gggcgaccgt aaattccccc gtacccgcca gcgccatcgg tagcaccagt tggtcggcga
    51001 gatattcccc caccgccgcc gggcttgcca ggtagcgttt cacctctttc accaactgtg
    51061 ccgcgaccac ctcggcactg acgcgctttt caccgacgac aaaaaagcgt tcggtgatat
    51121 tttcactttc gacttcaagc gagacggtat tacccggccc ctggtcgcgc ggcaggttat
    51181 gaatattctg ttcatgcaag gaaaaactac ccgccagtgt agcgatttca cgctcagcaa
    51241 catggcgcgg cacaccagcc agtagcacct ctccacgcat ctgcacaatg ttcccgcgct
    51301 cgccaagttg caagctatta aacgatgcca ccggtgagac ttccgttgct accatgccgc
    51361 ctccggcagg ataaaaaccg tgacgtaaca gcgtggtttg ctgatgaact cctattttcg
    51421 ccagcagcgg ctccagcacc cgacggataa aatccgcagg cggggccgac gggttatcgg
    51481 tgccgccgct cacttcaaca cgcgaaggtc catcggcaaa ccacagcgcg ggcagcaccg
    51541 tttgcagcac cagcgtacaa cttccggcgc taccgatagc aaagcggtaa tcgccgccgc
    51601 gcacggtgcc aggtcggaag accagacgct gcgaccccag ctcagcgcct tccaccgttg
    51661 ccctacaaat ttccgcagcc gcttttaccg cggtcagatg ttggcgcaac agccccggtt
    51721 tcgcccgccc ggcacgaatg ccggtgatgg taaatggctg gccggttatc atcgacaggc
    51781 tcagcgccga gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcacaatca
    51841 tccttttcat catttatcct tttacgcaca ccacctgacg cagggtatag ataacttcca
    51901 ccagatcgct ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt
    51961 cgatcacctc ggcatcttta cggcactcca catgcgcggt ggcacggatt tgatcttcca
    52021 cactgaacag ttttttcgct ttagtacggc tcattacccg cccggcaccg tggctgcacg
    52081 aacagaacga ctcttcattt cccagcccac ggacgataaa gcttttcgcc cccatcgaac
    52141 cgggaataat tccatattga ccagcacgcg cagacaccgc gcctttacgc gtcacataga
    52201 tctcttcgcc aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca
    52261 tcgccagcgt ttgtggctgt tttaccgttt tctgcgtaac gctctgcaac gccgttacca
    52321 cgttttccat catcgcatcg cggttaaggc tggcaaaaag ctgcgcccag gccacggctt
    52381 tcaggtaatc atcaaagtat tccgtacctt ccataaagta cgccaggtca cgcgatggca
    52441 acgtttcaag cgtttcctgc atctcttttt gcgccagatc gataaaataa gttccgatgg
    52501 cgttaccaat tccgcgtgaa ccggagtgca gcataatcca cacctgatcc gactcatcaa
    52561 ggcaaatttc aataaagtgg ttacccgtac ccagcgttcc caggtgttta tagttattag
    52621 tgttaaggaa acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc
    52681 atttcgcatc gacgttaaca ggcggatttt cccatgcgcc tttatcacgt ttacaacgcc
    52741 cggtagtacg tccgtgcggt acggccgttt caatcgcctg acgcagctct gccaggtttt
    52801 ctggcaggtc cgccgccgtt aacgcggtac gcagtgcgtt cattccacag ccaatatcca
    52861 cgcccaccgc cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac
    52921 ctttacccag gtgtacatca ggcattaccg caatatgttt gaaaataaat ggcatcttcg
    52981 cggtattaat aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt
    53041 ttaccggagc attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt
    53101 gtttgctgtc ctgataattg caaccgtcgt gccagaaaat caaataagca tcttaatttt
    53161 ttaattcatt gtttttaaaa atattatatt tttacgtccg taaacggaga tttcccgcaa
    53221 agccgattta ccgataatga aatctcgtct tttataagga tatctaagat gcgtaaaaca
    53281 gtggcttttg gctttgtcgg aacggtgctg gattatgccg ggcgcggcag tcagcgctgg
    53341 tcaaaatggc gtccgacact ctgtctatgc cagcaagaat cgttagttat cgatcggctg
    53401 gaattattgc acgacgcccg ctcgcgctcg ctgttcgaaa cgcttaaacg cgatatcgcc
    53461 agcgtttcgc cagaaacaga agtggtgggc gttgagatag aactgcataa cccttgggat
    53521 ttcgaagagg tttacgcctg cctgcatgat ttcgcccgtg gttacgcgtt ccagccagaa
    53581 aaagaagact atttaattca catcaccacc ggtacccacg tcgcgcagat ttgctggttt
    53641 ctgctggcag aagcgcgtta cctgcccgcc cggttgatac aatcttcacc accgcgcaaa
    53701 aaagagcggc ccgatagccc tggcgcagtg acgattatcg atctcgattt aagccgttat
    53761 aacgccatcg ccaaccgctt tgccgaggaa cgccagcaaa cgcttgattt tcttaagtcc
    53821 ggcattgcca cgcgtaactc ccacttcaac cgcatgattg agcagattga aaaagtggcg
    53881 ataaaatccc gcgcaccgat cctgcttaac ggcccgaccg gtgcaggtaa gtcatttctg
    53941 gcacgacgca tcttcgagct aaaacaggcg cggcatcagt ttagcggcgc gtttgtggaa
    54001 gtgaactgcg ccaccctgcg cggcgatacc gccatgtcga cgctgtttgg tcatgtgaaa
    54061 ggcgcgttta ccggggcgcg ggaatcgcgt gaaggattat tacgcagcgc caacggcgga
    54121 atgttgtttc ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa
    54181 gccattgaag agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt
    54241 cagcttatcg ccggaacggt gcgcgatttg cgccagttgg ttgccgaagg caaatttcgt
    54301 gaagacctgt acgcgcgaat aaatctctgg accttcacct tgccaggatt acgccaacgc
    54361 caggaagata ttgaaccgaa cctggattat gaagtggggc gccacgcctc actcactggc
    54421 gacagcgtgc gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc gacctctccc
    54481 caggcaacat ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc
    54541 tttgccacta gcggacgcat cacactggat acggtggaag atgagatcaa ccgtctgcgc
    54601 tataactggc aggagagtcg cccctccacg cttacggcgt tgctgggcgc agaggcagaa
    54661 aacatcgatc tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca
    54721 aagtcgcttt ctgctgccgg acgtgaactg tttgacattt cgcgccaggg taaagccagc
    54781 gtcaatgacg cggatcggct acgcaaatac ctggcgcgtt ttggtctgac atgggaagca
    54841 atgcaggatc agcacagctc cagttgaata tgatggtcct tcagcacctg catcacgctc
    54901 gcaggcggca tcgtgtcggt gtagacagca tcgaccatgc tgatgctacc catattgacc
    54961 attgcgttac ggccaaattt tgagtggtcg acaaccagca taacgtggcg cgagttctca
    55021 atgatggcgc gtttggtgcg aacttcgtgg taatcgaact ccagtagcga gccgtcgcta
    55081 tcgatgccgc ttatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc
    55141 gttgcttcgc caatgatccc gccatcgcgg ctgcgtaatt cgccaccggc aagaatgata
    55201 cggaaatcct cttttaccat cagcgtgtta gcgacgttga gattgttggt cacaatgcgt
    55261 aaattgctgt gattaagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata
    55321 aacagcgtcg agccattggg aatttgctcc gccactttgc gggcgatgcg ctctttttct
    55381 tcggtctggg tggccttgcg atcgtgccac ggcgtattaa ccgaactgga aggcagcgcc
    55441 gcaccgccat gatggcgcag gatcaggttt tgctctgcca gctcgttgag atcgcggcga
    55501 atagtctgcg ggctaacgga gaaatgctct accagctcct cggtgctgac ataaccctgc
    55561 tgtttaacca gttcgataat accgttgtga cgttgtgttt gtttcattga taaatccctg
    55621 gaattatttt cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc
    55681 taacccggcg atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc
    55741 gacaatccag atcaacgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg
    55801 atcgcgttcg ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc
    55861 gccaaaccac ggcccgctga atttttgctg cacatagccg cttaacaggg cgctaatgag
    55921 agtaatgaca attagcttac cgccaccgag gcgtttttcc accgcaccgc cgagatacca
    55981 ccaccagagc aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa
    56041 gtaacgccag aactcaaact tcagcgtcgg atcgaatggc caggccagcc ataacatcac
    56101 ttcctgatcg ccgagaattt gcatggcgat aaacaccacc acgcaggcga tcatcatcac
    56161 ccaggttacc ggacctgcgc gttcacgcaa ggcggcaaag aaaggataac ggcgataatg
    56221 caggccactg ccggtatggc ccgcctgcca gctggccgcc agataacgcg gatctgccgg
    56281 gttttcgaga aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag
    56341 ccagacatcg ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta
    56401 atcaacaaac gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc
    56461 tgtcgcttat tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct
    56521 gcgggaaact ggcgctgcca ggcttcaaaa ccgccatcaa tgctgtagac cacatcgtag
    56581 ccctgttgca gcagatactg cgccgcgcct ttgctgctat tgccgtgata acacatcacc
    56641 atcaccggag tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa
    56701 tggaaagcct gcaccgcatg ccccattgcg aaactctgtg gatcgcgaat atcgaccagc
    56761 accgcctctt tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga
    56821 tccatgcgtc tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga
    56881 agcggcagat aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac
    56941 ccaaagcgcg attctttgct aatatgttcg ataacgaaca tttatgaact ttaacgaaag
    57001 tgaatgaggg cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg
    57061 ctggtatcgc ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg
    57121 atctcgcttg cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc
    57181 ttgagcacta tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctactga
    57241 aaatggcccc gcatatcgct ttcccgatgc gttttcgcct gccgcatcgt ccgcatctac
    57301 gcccggcgtg gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca
    57361 gcctgccggg atcaaccggt ttgcgttttg gcgcaaactc agtgttaaaa ccggaaatta
    57421 agcgcggatt cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg
    57481 cccagatggt ggtgcgtaaa ggcggtgaag tgcttactcg tactcgtgcc acgtctgctc
    57541 gccgcgaaaa cggcctgtgg attgtggagg cggaagatat cgataccggc aaaaaatata
    57601 catggcaggc gcgcggcctg gttaatgcta ccggcccgtg ggtgaaacaa tttttcgacg
    57661 acgggatgca tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg
    57721 tgccgcgtgt gcatacccag aagcaagcct acattctgca aaacgaagat aaacgtattg
    57781 tcttcgtgat cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca
    57841 aaggcgatcc aaaagcagtg aagattgaag agagtgaaat caattacctg ttgaaagtgt
    57901 ataacacgca ctttaaaaag cagttaaacc gggacgatat cgtctggacc tactccggcg
    57961 tgcgtccgct atgtgatgat gagtccgact cgccacaggc cattacccgt gattacaccc
    58021 ttgatatcca tgatgaagac ggcaaagccc cgctgctgtc ggtattcggc ggtaagctga
    58081 ccacctaccg caaactggcg gagcacgcgc tggaaaaact gacgccatat tatcagggca
    58141 ttggtccggc gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgtg
    58201 acgattacgc cgcccgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc
    58261 actacgcgcg cacttacggc agcaacagcg aactgctgct cggcaatgca ggggcgataa
    58321 gcgacctcgg tgaagatttc ggtcatgagt tctacgaagc ggagctgaaa tatctggtcg
    58381 accacgaatg ggtccgccgc gccgacgatg ccctgtggcg tcgcacgaaa caaggcatgt
    58441 ggctaaatgc ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga
    58501 ggttgtcgct ggcgtcgtaa ggatgatgcc cggttgtgaa gtgcaaccgg gctatttgaa
    58561 ttccttagac tattcattgt aaataattga ataacggccc cagagctaaa atcgccaaac
    58621 ttaagcacgt tacccaatcg ccccacttct tcctatggct tctatttttg ctacgccact
    58681 ggcaataata tcccaacgca aaaccaagct gggcactaat cgggccgaca aaatcagtcc
    58741 acggctgcgg ccaaaaaggg gaaaaattca ggggaagcca gacaaatgtc agaaagaaat
    58801 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
    58861 gaattaacca gaaatagacc ggagaaaaga ccagccagca atgtagcctg ttttttaatt
    58921 caggaggcgt tctgcgggag aattgccaac cgatgccacc cattacactg aaaaaaagtg
    58981 catattcaac caataactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
    59041 gaaaggagaa tattatttcc gccagaaaag ttaacgtgaa tataagtacg gctaaggcaa
    59101 taaccgtcgg ccataccaat aatggtacga taacaaaacc gagaattata gggggaatat
    59161 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
    59221 gcataaacac tcatcaatca tccagtcgtc ttcccagatc tgatactgtg tgccctgttc
    59281 gggctgctta agcgggttct caatcgcctg ccgcgcgcgt tctttcagga tggattgatt
    59341 gttgaaggcc caggcattga tttgggcggt aatggcttta tcgatatcgt cctggttatc
    59401 tctgatataa ccgtcacgat acgtattcaa taaacaggca agagacgctg ccgaccaaaa
    59461 tccgggaact gaaataatgg catcaagcaa tggcgaaaga gcgatatcgg cacggaaaaa
    59521 catttccagc ttgtactgta attcgacagc ctcatccccg gatacccaat cagtcatata
    59581 ctggcaagca aattgctgca atatttcccg ttcatcctca cgccagtttt ctttactggc
    59641 aaggtttata cgccgtagag aaaatacttc gtgaatattg tcaaattgcc cctgcgcaac
    59701 aaactccagt aaacgaggca gaaaataacg aacctcatcg ctgttttgcg gatcaggtcc
    59761 tggactacta ttccaggcat tgattaagga ataaggaatc gctcgcaacg atgtattccg
    59821 taatgctttt tgttcctgtt gactcagaca atattcacaa caaaccacaa attgcttcgg
    59881 cgccggataa cgacgaaaaa tatgatatat ctgctcaatt aatgcctgca tggcaggagt
    59941 ggggtgcata attaccccct cgcccgctga tcgcgtgtaa agggttgata gacaaaacga
    60001 gcattcgccg cattcaggcg aataatggca ctacgagaga gggaaaagct gggccgaatg
    60061 caataattac agtcccaaca gctttcctct gtgcaatttt ccactgagta atgacgcgcg
    60121 tagagtttca gttcagcctg cttatcgcga aatccgcgta aactgttatc gttttgaaat
    60181 tgccaggctt ctttaaacac ttgaaagata taaacatcct caatccaggc gtcacatttt
    60241 ttaagcggac gctttgctat gatcacccgc tcatccgggt tgagcatcgc cgtaaattgt
    60301 acggtgaagt catttgtcgt gttcagatca cattgaacat ctggtacatc actgatatag
    60361 aggtcaaatc caaagtcagc accacaggca caaagacggc taatggtggc ggtatcgagg
    60421 gaaaatcccg gccatacatt gctggacgtt aaaccaatat ttaatgtgac atcatatcga
    60481 gataatccct caagaccggc agcaccggcg aacttttccc cttcatccag gacctggtta
    60541 atgagttgct gaacaatgcc ttcatcccag tatgttgatt tgcagttggc aatc
//
LOCUS       DADSZD010000007        60068 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.007, whole genome shotgun sequence.
ACCESSION   DADSZD010000007 DADSZD010000000
VERSION     DADSZD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.007"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            1..948
                     /locus_tag="J1Z28_000359"
                     /pseudo
     CDS             1..948
                     /locus_tag="J1Z28_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(968..1078)
                     /locus_tag="J1Z28_000360"
     CDS             complement(968..1078)
                     /locus_tag="J1Z28_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA7715228.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHAC"
     gene            complement(1136..2155)
                     /locus_tag="J1Z28_000361"
     CDS             complement(1136..2155)
                     /locus_tag="J1Z28_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HBA7715229.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFELDQKHCCKVL
                     LTFSE"
     gene            complement(2167..3381)
                     /gene="rspA"
                     /locus_tag="J1Z28_000362"
     CDS             complement(2167..3381)
                     /gene="rspA"
                     /locus_tag="J1Z28_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBA7715230.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(3587..3913)
                     /locus_tag="J1Z28_000363"
     CDS             complement(3587..3913)
                     /locus_tag="J1Z28_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBA7715231.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4048..4389
                     /locus_tag="J1Z28_000364"
     CDS             4048..4389
                     /locus_tag="J1Z28_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBA7715232.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            4424..4984
                     /gene="speG"
                     /locus_tag="J1Z28_000365"
     CDS             4424..4984
                     /gene="speG"
                     /locus_tag="J1Z28_000365"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HBA7715233.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLAAR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(4987..5696)
                     /locus_tag="J1Z28_000366"
                     /pseudo
     CDS             complement(4987..5696)
                     /locus_tag="J1Z28_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
     gene            5804..6109
                     /locus_tag="J1Z28_000367"
     CDS             5804..6109
                     /locus_tag="J1Z28_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBA7715234.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
     gene            6308..8734
                     /locus_tag="J1Z28_000368"
     CDS             6308..8734
                     /locus_tag="J1Z28_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HBA7715235.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAEAAV
                     QQANEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVKQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     SAFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDLVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            8849..10735
                     /locus_tag="J1Z28_000369"
                     /pseudo
     CDS             8849..10735
                     /locus_tag="J1Z28_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            10746..11363
                     /gene="dmsB"
                     /locus_tag="J1Z28_000370"
     CDS             10746..11363
                     /gene="dmsB"
                     /locus_tag="J1Z28_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBA7715236.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            11365..12219
                     /locus_tag="J1Z28_000371"
     CDS             11365..12219
                     /locus_tag="J1Z28_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBA7715237.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            12262..12876
                     /gene="dmsD"
                     /locus_tag="J1Z28_000372"
     CDS             12262..12876
                     /gene="dmsD"
                     /locus_tag="J1Z28_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBA7715238.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTNDSWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            13036..14328
                     /gene="clcB"
                     /locus_tag="J1Z28_000373"
     CDS             13036..14328
                     /gene="clcB"
                     /locus_tag="J1Z28_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBA7715239.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLISNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWLPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(14281..14976)
                     /gene="bioD"
                     /locus_tag="J1Z28_000374"
     CDS             complement(14281..14976)
                     /gene="bioD"
                     /locus_tag="J1Z28_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBA7715240.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(15101..16321)
                     /gene="mlc"
                     /locus_tag="J1Z28_000375"
                     /gene_synonym="dgsA"
     CDS             complement(15101..16321)
                     /gene="mlc"
                     /locus_tag="J1Z28_000375"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBA7715241.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLERIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(16456..17349)
                     /locus_tag="J1Z28_000376"
     CDS             complement(16456..17349)
                     /locus_tag="J1Z28_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7715242.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            17456..18709
                     /locus_tag="J1Z28_000377"
     CDS             17456..18709
                     /locus_tag="J1Z28_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715243.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            19107..19442
                     /locus_tag="J1Z28_000378"
     CDS             19107..19442
                     /locus_tag="J1Z28_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBA7715244.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQP
                     AKPAAQPAA"
     gene            19535..19618
                     /locus_tag="J1Z28_000379"
     CDS             19535..19618
                     /locus_tag="J1Z28_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715245.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            19718..20539
                     /locus_tag="J1Z28_000380"
     CDS             19718..20539
                     /locus_tag="J1Z28_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBA7715246.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20578..20907)
                     /gene="mdtI"
                     /locus_tag="J1Z28_000381"
     CDS             complement(20578..20907)
                     /gene="mdtI"
                     /locus_tag="J1Z28_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBA7715247.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRCKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(20894..21259)
                     /gene="mdtJ"
                     /locus_tag="J1Z28_000382"
     CDS             complement(20894..21259)
                     /gene="mdtJ"
                     /locus_tag="J1Z28_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBA7715248.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            21670..22704
                     /gene="tqsA"
                     /locus_tag="J1Z28_000383"
     CDS             21670..22704
                     /gene="tqsA"
                     /locus_tag="J1Z28_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBA7715249.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(22729..24117)
                     /gene="pntB"
                     /locus_tag="J1Z28_000384"
     CDS             complement(22729..24117)
                     /gene="pntB"
                     /locus_tag="J1Z28_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBA7715250.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(24128..25660)
                     /gene="pntA"
                     /locus_tag="J1Z28_000385"
     CDS             complement(24128..25660)
                     /gene="pntA"
                     /locus_tag="J1Z28_000385"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBA7715251.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            26184..27128
                     /locus_tag="J1Z28_000386"
     CDS             26184..27128
                     /locus_tag="J1Z28_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7715252.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            27314..28696
                     /locus_tag="J1Z28_000387"
     CDS             27314..28696
                     /locus_tag="J1Z28_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7715253.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASTLLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            28733..29455
                     /gene="folM"
                     /locus_tag="J1Z28_000388"
     CDS             28733..29455
                     /gene="folM"
                     /locus_tag="J1Z28_000388"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBA7715254.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLMNAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSLPLDGGRHLR"
     gene            complement(29452..29787)
                     /locus_tag="J1Z28_000389"
     CDS             complement(29452..29787)
                     /locus_tag="J1Z28_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBA7715255.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            29916..30635
                     /gene="rstA"
                     /locus_tag="J1Z28_000390"
     CDS             29916..30635
                     /gene="rstA"
                     /locus_tag="J1Z28_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBA7715256.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVSLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            30639..31940
                     /gene="rstB"
                     /locus_tag="J1Z28_000391"
     CDS             30639..31940
                     /gene="rstB"
                     /locus_tag="J1Z28_000391"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBA7715257.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLSERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            32016..32945
                     /gene="tus"
                     /locus_tag="J1Z28_000392"
     CDS             32016..32945
                     /gene="tus"
                     /locus_tag="J1Z28_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBA7715258.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQSALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(32942..34345)
                     /gene="fumC"
                     /locus_tag="J1Z28_000393"
     CDS             complement(32942..34345)
                     /gene="fumC"
                     /locus_tag="J1Z28_000393"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBA7715259.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVDSMKAGG"
     gene            complement(34488..36134)
                     /locus_tag="J1Z28_000394"
     CDS             complement(34488..36134)
                     /locus_tag="J1Z28_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBA7715260.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAILAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            36333..37508
                     /gene="manA"
                     /locus_tag="J1Z28_000395"
     CDS             36333..37508
                     /gene="manA"
                     /locus_tag="J1Z28_000395"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBA7715261.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPDDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKM"
     gene            37609..39117
                     /locus_tag="J1Z28_000396"
     CDS             37609..39117
                     /locus_tag="J1Z28_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBA7715262.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPTLNVPAVPAIPQQ"
     gene            complement(39162..40427)
                     /gene="uidC"
                     /locus_tag="J1Z28_000397"
     CDS             complement(39162..40427)
                     /gene="uidC"
                     /locus_tag="J1Z28_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBA7715263.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGTGRDIYAWVQGGLLDFNSGYYSNIIGVEGGVYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(40466..41839)
                     /gene="uidB"
                     /locus_tag="J1Z28_000398"
     CDS             complement(40466..41839)
                     /gene="uidB"
                     /locus_tag="J1Z28_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBA7715264.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGAVCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            complement(41836..43647)
                     /gene="uidA"
                     /locus_tag="J1Z28_000399"
     CDS             complement(41836..43647)
                     /gene="uidA"
                     /locus_tag="J1Z28_000399"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBA7715265.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQLADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVGANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(44038..44628)
                     /locus_tag="J1Z28_000400"
     CDS             complement(44038..44628)
                     /locus_tag="J1Z28_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7715266.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(44849..45616)
                     /gene="hdhA"
                     /locus_tag="J1Z28_000401"
     CDS             complement(44849..45616)
                     /gene="hdhA"
                     /locus_tag="J1Z28_000401"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBA7715267.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(45728..46756)
                     /locus_tag="J1Z28_000402"
     CDS             complement(45728..46756)
                     /locus_tag="J1Z28_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mal regulon transcriptional regulator MalI"
                     /protein_id="HBA7715268.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGESGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECSSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            46931..48523
                     /locus_tag="J1Z28_000403"
     CDS             46931..48523
                     /locus_tag="J1Z28_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBA7715269.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            48533..49705
                     /gene="malY"
                     /locus_tag="J1Z28_000404"
     CDS             48533..49705
                     /gene="malY"
                     /locus_tag="J1Z28_000404"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBA7715270.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQPDGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            49809..50810
                     /gene="add"
                     /locus_tag="J1Z28_000405"
     CDS             49809..50810
                     /gene="add"
                     /locus_tag="J1Z28_000405"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBA7715271.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNDLHYVELRFSPG
                     YMAMAHQLPVSGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(50846..51886)
                     /locus_tag="J1Z28_000406"
     CDS             complement(50846..51886)
                     /locus_tag="J1Z28_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA7715272.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEECLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            52129..52254
                     /gene="blr"
                     /locus_tag="J1Z28_000407"
     CDS             52129..52254
                     /gene="blr"
                     /locus_tag="J1Z28_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBA7715273.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            52527..52742
                     /gene="ydgT"
                     /locus_tag="J1Z28_000408"
     CDS             52527..52742
                     /gene="ydgT"
                     /locus_tag="J1Z28_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBA7715274.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            52828..53268
                     /locus_tag="J1Z28_000409"
     CDS             52828..53268
                     /locus_tag="J1Z28_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBA7715275.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            53345..53926
                     /gene="rsxA"
                     /locus_tag="J1Z28_000410"
     CDS             53345..53926
                     /gene="rsxA"
                     /locus_tag="J1Z28_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBA7715276.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            53926..54504
                     /gene="rsxB"
                     /locus_tag="J1Z28_000411"
     CDS             53926..54504
                     /gene="rsxB"
                     /locus_tag="J1Z28_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBA7715277.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAPE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            54497..56722
                     /gene="rsxC"
                     /locus_tag="J1Z28_000412"
     CDS             54497..56722
                     /gene="rsxC"
                     /locus_tag="J1Z28_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBA7715278.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDDATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQIDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDPRKAAVE
                     AAIARAKARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQQANAEPEEQVD
                     PRKAAVEAAIARAKARKLEQQQANAEPEEQINPRKAAVAAAIARVQAKKAAQQKVVNE
                     D"
     gene            56723..57781
                     /gene="rsxD"
                     /locus_tag="J1Z28_000413"
     CDS             56723..57781
                     /gene="rsxD"
                     /locus_tag="J1Z28_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBA7715279.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDTIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLATLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            57785..58405
                     /gene="rsxG"
                     /locus_tag="J1Z28_000414"
     CDS             57785..58405
                     /gene="rsxG"
                     /locus_tag="J1Z28_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBA7715280.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            58409..59104
                     /locus_tag="J1Z28_000415"
     CDS             58409..59104
                     /locus_tag="J1Z28_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBA7715281.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAA
                     AERALPNGETGNV"
     gene            59104..59739
                     /gene="nth"
                     /locus_tag="J1Z28_000416"
     CDS             59104..59739
                     /gene="nth"
                     /locus_tag="J1Z28_000416"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBA7715282.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT        14916 a        15414 c        14617 g        15121 t
ORIGIN      
        1 tcaaaaatat cctgagcata atcggtgaaa aaaatcttgc ctcatcgatt aataaagaaa
       61 aattactgga ggttcgtaaa gagctactga ctggatacca gatccccaaa agtaactata
      121 ttgttacaca accagggaga tcggctgtaa ctgtaaataa ttacatgaca aatcttaacg
      181 ccgtgttcca gtttggtgtt gataacggtt acctggcaga taatccgttt accattaaag
      241 gaatcaagaa ccattccgga tcctctttcg cgggaggaat ttatccgtct tatcgatgcg
      301 tgcagaaatc agcaagcaaa aaatttatgg tgtgtttctg tttatactgg cgttcgccct
      361 ggtgagctgt gtgcacttgg atgggaggac atagatctga aaaatggaac aatgatgatc
      421 aggagaaatt tagcaaaaga ccgtttcacg gtaccaaaaa cacaggcggg aaccaatcgg
      481 gtcattcatc ttattaagcc agcaatcgac gctctccgga gtcagatgac attaacgaga
      541 ctgagcaaag agcatatcat tgatgttcac ctcagagagt atggcagaac agaaaaacaa
      601 aaatgcacct ttgtttttca acctgaagtg tcagcgagag taaaaaatta tggtgaccat
      661 tttaccgttg actcaataag gcagatgtgg gacgcagcga taaaacgtgc cggactccgc
      721 catcgaaaat catatcagtc gagacatact tatgcctgct ggtcgctgac agctggtgct
      781 aacccggcat ttatagcaaa ccagatgggc catgcagatg cgcaaatggt atttcaggta
      841 tacggaaaat ggatgtctga aaacaataat gcacaggtag ctttgttaaa tacacagtta
      901 agcgagtttg ccccaaccat gccccataac gaagcaatga aaaattaatt taatatttat
      961 caaatagtta acacgcatga ctcttgaaat ccataaattc aagcgcagtg cccaaccatc
     1021 ccgatactgc tgctttcacc aaatccttag tacttctttc gtgtttttct attgtcataa
     1081 tggttatctc taaaaaagag gtaagatgcg tactacttac tcgccgttat tggtattatt
     1141 cagaaaaagt gagtaagact ttgcagcaat gtttttgatc cagttcaaat aaactaatgg
     1201 catccgcaac atgctggaaa tcaaatgtat gggtaattag tttttctggt ttaattaacc
     1261 ctttacttaa ccagtcgata acgaccggga atttatttgc atttaagcgt gaagagaaaa
     1321 tagagagttc ttttccggta attccttgct gaatcacttc agacggttca ctggagaagc
     1381 ccatcaatac aatacgtgcc gctggagaag ccagcgttac ggcctctttc aagattgaag
     1441 gatgacaagc ggcatcgata attaaagtcg gcttgatgcc tttttcagcg aaactctcgc
     1501 caagcggtgt ctggctgtta ttaatcgccc agtctgcccc gctctctttc gctttttcca
     1561 gtcgttcatc aatgcgatcg gcaacaatca cattcttaac gttatagacg ccttttaata
     1621 cctgaacgat cgtcaggccg attggacctg caccgtaaac cagaacggta tcattttcag
     1681 tcggttgacc atgaccggta acgttagccg caatggtaaa aggttcaatc atcaccgcat
     1741 attgatcggc cacagcttca ggaattttcc acgcattttt tgccggcacc acggcatatt
     1801 cactgaaacc accgtcagcg tgcacaccta atacagccag tgtcgtacaa acgttcggct
     1861 tacctataga gcacggatag caatgcccac agctgaccac cggatcgaca gcaacgcgtt
     1921 caccgactct ggcgctttcc acgccttcac ccactgcatc aatgacgcca aaaaattcat
     1981 gtccaatgac gcgtggatat ttcgcaaaag gattatgccc acggtaaata tggctatctg
     2041 aaccacaaat tccggcaagt ttcactttta ctcgtacttc acccgctgac ggggtgggta
     2101 tttcacgttc gataatcgac agttgattcg gtttttcaat taaaatgctt ttcattacct
     2161 tactccttac cagttccaca gcgtgccatc ttccagacgt gcgactggca gataagcagg
     2221 ttcataggga tatttcgccg ccagcttttc atcgaattcg atgccaagac ccggtttttc
     2281 tcccggatgc atatagccgt tatcgaaagt ccagttgtgc gggaagactt cgagcatttg
     2341 ttcggaataa cccatgtatt cctggacacc gaaattgggg acccacagat caaagtgcag
     2401 cgccgcagcc atgcagactg gtgacaaatc ggaaggaccg tgtgagccag tacgtacctg
     2461 atacagcgaa gcaaaatcgg caatccgacg cataccggta attccgcctg catgggtcag
     2521 cgtggtacgg atataatcga tgagttgctc ttcaatcagt tgtttgcagt cccagatgct
     2581 gttgaagact tcacccaccg cgatgggtgt gacggtatgt tggcgaatga gacggaagca
     2641 ttcctggttt tccgcaggcg tcgggtcttc catccagaac atgcgataat cttcaatgct
     2701 cttaccaaag cgcgccgctt caataggcgt taagcgatgg tgcatgtcat gcagcagatg
     2761 ttcatcaaaa ccaaacttgt tacgtaccgc gtcaaacaat ttcggcatga aatcgaggta
     2821 tttctccgtc gaccacagct gctcttccgg ccactgtcct ttggttgcgg gttcataagc
     2881 cagaccttta cctttcgaca tgccgtaggt ggttttcata ccagggattc cgcactgcac
     2941 gcggatggct ttgaatccca gctcctgatg acgggcataa tcatccagag cttcatcaat
     3001 actgtgaccg gtggtatggc aataaaccat caccccttca cgagacgcgc cgccgagtaa
     3061 ctgataaagc ggcatgttgg cagctttggc tttaatatcc cacagcgcca tatcaaccgc
     3121 tgaaatggcc gacatcgtaa ccggaccgcg acgccagtaa gcacctttat agaaaaactg
     3181 ccagatatct tcgatacggt gtgcatcgcg accaataagc tgcggacaaa ggtgatcctg
     3241 caaataagag gccacggaaa gctcacgtcc atttagggtg gcatccccaa ggcccgtaat
     3301 accgtcctca gtggtgattt ttaatgtgac gaaattacgc cccggacagg taacaaaaac
     3361 ttcagccttt acgatcttca tgttcgattc cttgcatcgc ttgtcgtgat gcatgaaatc
     3421 tacgcaactg agctactacc atacaagtat gaagatcaaa aaaagccgga gtgatcacaa
     3481 aaaaaggcgt atatttgcgc cgtgaatggt tgacaaaaga tgaaacagaa taccttttgt
     3541 cagctgacac ttcctcgtat cttattgata aaatggattt atgttcctac gtgcgccccc
     3601 agcccgcaac aatgatcaac atgccgcaaa gcgcaattaa cgcccccgtc cagtcataaa
     3661 gagtcagttt cacgccatcc acaacgcgca gccaaatcaa cgccgtgcag acataaacgc
     3721 caccataagc cgcgtaaaca cgcccactcg ccgcaggatg caacgttaac aaccagacaa
     3781 acagcgccag tgaaatcccc gccggaagca acagccagat actggcgttt cgcttcaacc
     3841 acagccaggg caaaaagcat ccaataattt cacacagcgc agtagcaaaa aatagtaacg
     3901 ttgttttaat catctttgtc acttattgac atcacgtata gttatagggc gacataatat
     3961 catcaatata aacaccctcc ttgaacgttt tgcgttcgca gtggatggtg tagaatcagc
     4021 tttaatcatt catacaggga atgaattatg aaaatcactc tcagcaaacg aatcggcctg
     4081 ctcgcttttc tgctgccttg cgcactggca ttgagcacaa ctgttcatgc cgaaactaac
     4141 aaactggtga ttgagtctgg cgacagtgca caaagccgcc agcgcgccgc tatggaaaaa
     4201 gagcaatgga atgacacgcg taatctgcgc cagaaagtga ataaacgcac tgaaaaagag
     4261 tgggataaag ccgacgctgc ttttgataac cgcgataaat gtgagcaaag cgccaacatc
     4321 aatgcctact gggagccaaa tactttgcgc tgcctggacc gtcgaactgg ccgcgttatt
     4381 atcccctaac ctgttattga tttaaggaat gtaaggacac gttatgccaa gcgcccacag
     4441 tgttaagcta cgcccgctgg agcgtgaaga tttacgctat gtacatcaac tcgacaataa
     4501 cgccagtgtg atgcgttact ggtttgagga accctacgaa gcctttgttg aactctctga
     4561 tctgtatgat aagcatattc acgatcagag cgaacggcgc tttgtggtgg aatgtgacgg
     4621 cgaaaaagcc ggtctggtgg agctggtgga aattaaccat gttcatcgcc gcgcagaatt
     4681 tcagataatt atctccccgg agtatcaggg gaaaggtctg gcagcccgtg ccgccaaatt
     4741 agcaatggac tatggcttca ccgttctcaa tctctataag ctgtatctga tcgttgataa
     4801 agagaatgaa aaagcgattc acatttaccg caagcttggc tttacggttg aaggtgaatt
     4861 gatgcatgag ttctttatta atggtcaata tcgtaatgcc attcgcatgt gtatattcca
     4921 gcatcagtat ctggctgagc acaaaacacc gggtcagact ctcctgaagc cgaccgcaca
     4981 atagtattaa taataatcga tcgtattttt gatggtgtaa acccgttcga cggcgggttt
     5041 tactccttca tcaacaatga ttagctgaca gtccaccgga ttggcgtgat tgtcatattc
     5101 acagctctgt tttacattac caacccgttg attattcagt aaagtaaccg ctgtgtaatc
     5161 taattttttg atcgggtccg ttgatggcgt ggcgctgaca gataatgttt tatcgttact
     5221 tgttgtggtt ttacccagcg gatacccctg atcatcatag cgatattcca tctgcatctg
     5281 tttgctgctg gctttaatca cgaaaccatt atcatcggtt tcccaactca ccccgcagaa
     5341 ggtaattctg ctagctggca ttttccctgc aaacgaactc ttttctccag cgtctcggca
     5401 tcacggtaat aattggcgtc cagtaccagg gcaaccacgg tattattttc cagatccagt
     5461 aattcgagag aatcaaaaca gccttcttcc gacaaagtcc ccgaaacacg tttcgtcact
     5521 tcaccttgct catccattaa tgtctgagtg aaatctttta cgggaccacg cagcggatca
     5581 aaatcgaatt cattagagaa actggccatc tccgggctaa atgaaagtgt tacctctgtg
     5641 cggtcacatc ctgtgagtaa tatcgcgagt aagcatggca gtaatttgta tttcacaaca
     5701 gtcacctgag agtagagatg attctcaatc atagtagcaa atacagtact ttacacgtta
     5761 aatgctatgc ttaaagaagt tatcttcgcg taaggagctt atgatgaaac tctcaacctg
     5821 ctgtgccgcg cttctgctcg ccctcgcctc acccgcggta ctcgccgcgc cgggatcctg
     5881 tgagcgcata cagagcgata tatcacagcg cattatcaat aatggcgtac ccgaaagcag
     5941 cttcacgtta agtattgtac ccaatgacca ggttgatcag cctgattccc aggtcgtcgg
     6001 gcattgcgct aatgatacgc ataaaattct ctacacccgt accaccagcg gtaacgtctc
     6061 tgctcccgcg cagtctaccc aggataacgc gcctgctgaa ccgcagtaac acattatcga
     6121 ctgaacgccg gatacgacac atccggcgat ttgaacatac aacataatcc caccttatta
     6181 ctcataccct tctattgata tggattaata attattaacc ccaaatgggt agactctctc
     6241 tattgttagc gcgctaaata ttcaatatat aaacatttat ataacgataa agaacaggga
     6301 gtgagttatg tccaaaaatg aacaaatggt gggcatcagc cgcagaacac tcgttaaatc
     6361 taccgcgata ggttctctgg cgctggctgc aggcggtttt tctttgccgt ttaccctgcg
     6421 cagtgcagaa gcagcggtac aacaggccaa cgaaaaagtg gtctggggtg cctgttccgt
     6481 caactgtggt agccgctgtg cacttcgtct gcatgttaaa gataatgaag tgacctgggt
     6541 ggaaactgac aacaccggca gcgacgagta tggcaaccat caggtacgcg cctgtttgcg
     6601 cggtcgctcc atccgtcggc gtattaatca tcccgatcgc ctgaattacc caatgaaacg
     6661 cgtgggcaaa cgcggcgaag gcaaattcga acggattagc tgggatgaag ccctggatac
     6721 tatcgccagt agcctgaaaa aaaccgtcaa acaatatggc aatgaggctg tatatattca
     6781 gtactcttcg gggattgttg gcggcaatat gacccgctct tctccatcag cctcggcggt
     6841 caaacgcctg atgaactgct acggcggttc actaaatcag tatggctcct acagcactgc
     6901 gcaaatttcc tgtgccatgc cctacaccta cggcagtaat gatggcaaca gcaccacgga
     6961 tattgaaaac agcaagctgg tcgtgatgtt tggtaacaac ccggctgaaa cccgcatgag
     7021 cggcggtggc atcacttatc ttcttgaaaa agcacgcgag aaatcgaacg caaaaatgat
     7081 tgttatcgat ccgcgatata ccgatacggc tgctggtcgt gaagatgaat ggcttcctat
     7141 tcgcccgggc accgatgccg cgctggttgc gggtattgcc tgggtattga ttaacgaaaa
     7201 tctcgttgat caaccttttc tcgataaata ctgcgtcggc tatgacgaaa aaaccttacc
     7261 cgcagatgca cccaaaaatg gtcactataa agcctatatt cttggtgaag gcgatgataa
     7321 aacagcgaaa acgccgcagt gggcttcgca aattacaggt atcccggtag accgtatcat
     7381 caaactggcg cgtgaaattg gcacagcaaa acccgcttat atctgccagg gctgggggcc
     7441 gcaacgccag gcaaacggcg agctgacggc acgcgctatt gctatgttgc ctattttgac
     7501 gggcaatgtc ggcatcagcg gcggaaatag tggtgcgcgt gaatcgacct acaccattac
     7561 catcgaacgc ttgccggtgc tggataatcc ggtcaaaacg tcaatctcct gcttcagctg
     7621 gacagatgct atcgatcatg gcccgcaaat gacggcaatc cgcgacggcg tccggggcaa
     7681 agataaacta gatgtgccca ttaagttcat ctggaactac gcgggaaata ccctggttaa
     7741 tcagcattct gacatcaaca aaacgcatga aattctgcag gacgaatcga aatgcgaaat
     7801 gattgtggtc atcgaaaact ttatgacctc atcggcaaaa tatgccgaca ttctgctgcc
     7861 agacctgatg accgttgagc aggaagatat tattcctaac gactacgccg gtaacatggg
     7921 atatctcatt ttcctccagc ctgtcaccag cgaaaaattc gaacgcaaac caatttactg
     7981 gatcctgagt gaagtcgcga aacgtcttgg acctgacgtc tatcaaaagt tcactgaagg
     8041 tcgcacgcag gaacaatggt tacaacatct gtacgccaaa atgcttgcca aagatccggc
     8101 gttaccgtct tacgacgaat taaaaaaaat gggtatctat aagcgtaaag atcccaatgg
     8161 tcattttgtc gcctacaaag catttcgtga cgaccccgag gcaaatccac ttaaaacgcc
     8221 ttccggtaag attgaaattt attccagcaa gctggcggaa attgcccgta cctgggaact
     8281 ggaaaaagat gaagtgataa gcccactgcc ggtttatgcc tcaacctttg aaggctggga
     8341 ctcccctgaa cgtagtgcct tcccactgca actgtttggt ttccattaca aatcccgtac
     8401 gcactcgacc tacggcaaca ttgatgtcct gaaggcagcc tgccgtcagg aagtgtggat
     8461 caaccctata gatgcacaga aacgtgggat tgccaacggc gatttggtgc gggtgtttaa
     8521 ccatcgtggc gaagttcggc taccagccaa agtgacacca cgcattctcc ctggggttag
     8581 cgctatgggc cagggagcct ggcacgaggc caatatgtct ggcgataaaa tcgaccacgg
     8641 cggctgtgtg aatacgctaa ccactctgcg cccttcacca ctggcgaagg gaaacccgca
     8701 gcacactaat ctggtcgaga tcgaaaaaat ataacccact acaaccataa attctggcat
     8761 gacattttgt ttgaaaagca ataagtgagt aatgatgaaa atccatacca cagaggcgct
     8821 catgaaggct gaaatcagcc gtagaagtct gatgaactct tgcggtggtt ttctcagtcg
     8881 ctatggcagc tacagtaccg cacagatcag tgccgcgatg agttatatgt tcggtgccaa
     8941 tgatggcaac agcccggatg atatcgccaa tacgaaactg gtcgttatgt tcggaaataa
     9001 cccggcagaa acccggatga gcggcggtgg tgtcacttac tacgtcgagc aagcccgcga
     9061 acgttcaaac gcacgcatga tcgtcattga tccacgttat aacgacactg ctgccgggcg
     9121 tgaagatgaa tggctgccca ttcgccctgg caccgatggc gcactggcct gtgcaattgc
     9181 ctgggtactg attactgaaa acatggtcga tcagccattt ctcgacaaat attgtgttgg
     9241 ttacgatgaa aaaacgctgc cagctaacgc tccacgtaac gcgcattata aagcctatat
     9301 tctgggcgaa gggcctgacg gcatagctaa aacgccggaa tgggcagcaa aaatcaccag
     9361 catcccggca gaaaaaatta tccagttggc acgagagatc ggttcagcaa aaccggctta
     9421 tatttgtcag gggtgggggc cacaacgaca ttccaacggc gagcaaacat cccgcgctat
     9481 tgccatgctt tccgttctca ccggtaacgt cggcataaac ggcggcaact caggtgtacg
     9541 cgaaggtagc tgggatctgg gggtagaatg gttcccgatg ctcgagaatc ctgttaaaac
     9601 gcagatttcc gtctttacat ggacagatgc tatcgaccat ggtacggaaa tgaccgcgcc
     9661 ccgtgatggt gttcgtggaa aagaaaaact ggatgtcccc atcaagtttt tatggtgcta
     9721 cgccagtaac acattgatca atcaacatgg cgacatcaat cacacccatg aggtgcttca
     9781 ggatgacagc aagtgcgaga tgattgttgg cattgaccac ttcatgactg cctcggctaa
     9841 gtattgcgat atcctgttac ccgacctgat gccaacagag caagaagacc tcatctctca
     9901 tgagtctgcc gggaatatgg gctatgtgat cctcgcccaa cccgcaacct cagcaaaatt
     9961 tgaacgcaaa cccatctact ggatgctgag tgaagtcgcc aaacgcttag gaccagacgt
    10021 ttatcaaacc tttactgaag gtcgcagtca gcatgaatgg atcaaatatc tccatgcgaa
    10081 aacgaaggaa cgtaaccctg agatgcccga ctacgaggag atgaaaacga ccgggatctt
    10141 taagaaaaaa tgcccggaag aacactacgt cgctttccgc gcattccgtg aagatccaca
    10201 ggcaaaccca ttgaaaacac cttcgggaaa aatcgaaatt tattctgaac gactggcgaa
    10261 gattgcggat acctgggaat tgaaaaaaga tgaaattatt catccccttc cagcttatac
    10321 gccaggtttt gatggctggg acgatcccct gcggaaaacc tttccactgc agctaacggg
    10381 cttccactac aaagcgcgta cccactccag ttacggcaat attgatgtat tacagcaagc
    10441 ctgcccacaa gaggtgtgga tcaaccctat tgatgctcag gcacgcggta tccgtcatgg
    10501 cgataccgtg cgggtattta acaataatgg agaaatgttg attgccgcga aagtgactcc
    10561 gcgtattctg cctggcgtta ccgccatcgg gcaaggtgcg tggcttaaag cggatatgtt
    10621 tggtgaccgg gtcgatcacg gcggcagtat caatattctg acctcgcacc gcccttcacc
    10681 gctggcaaag ggaaacccgt cgcacagcaa tcttgtccag atcgaaaagg tttaaggagt
    10741 agcccatgac cacacaatat ggatttttta tcgattcaag ccgctgtact ggctgtaaaa
    10801 cctgcgaact ggcgtgcaaa gattttaaag atcttggccc ggaagtcagt ttccgccgta
    10861 tttatgaata tgctggcggc gactggcagg aagataatgg cgtctggcac cagaacgtgt
    10921 tcgcttatta tctctccatc tcctgtaacc attgcgatga ccccgcatgt acaaaagtct
    10981 gccccagcgg cgcaatgcat aagcgtgaag atggctttgt ggtggttgat gaagatgttt
    11041 gtatcggctg ccgctactgc cacatggctt gtccgtacgg cgcgccacag tacaatgctg
    11101 aaaaagggca catgacgaag tgcgatggtt gttattcgcg cgtcgccgag gggaaacaac
    11161 ccatatgtgt cgaatcctgc ccactgcgtg cacttgagtt cggtccaatt gaagaattgc
    11221 gccagaagca cggcactctg gctgccgtcg cgccactgcc gcgcgcgcat ttcacaaaac
    11281 ccaatatcgt tatcaaacct aacgccaaca gccgcccgac cggcgatacc actggttatc
    11341 tggctaatcc ggaggaagtg taaaatggga aatggatggc atgaatggcc actggttatc
    11401 tttaccgtat tgggtcaatg cgtagtaggc gcgctgattg ttagcggaat tggctggttc
    11461 gccgccaaaa atgacgcaga ccgtcagcac attattcgtg gcatgttttt cctctggctg
    11521 ttgatgggca tcgggtttat tgcctctgtc atgcacctgg gatcgccact gcgggcgttt
    11581 aactcgctga atagaatcgg agcttctggt ctgagtaacg agattgctgc cggttccata
    11641 ttttttgccg ttggcggatt atggtggctg gtggcggtca tcggtaaaat gccgcaagcg
    11701 ttggggaaac tctggttact tgtcagcatg gcgctcggcg tcattttcgt ctggatgatg
    11761 acctgcgtgt atcaaatcga cactgtgcca acctggcata acggttacac tacgctggct
    11821 ttcttcctga cggtattgtt gagcggtcca atacttgctg ccacaattct acgtgcagcc
    11881 cgcgttacat ttaataccac accatttgcc atcattagca ttctggcgct gatcgcttgt
    11941 gccgggttga ttgtgctgca aggtttgtct ctggcgtcta ttcactcatc cgtacaacaa
    12001 gccagcgcac tggtaccaga ttacgcctcc ttacaggtct ggcgtgtggt attgttatgc
    12061 gcaggtcttg gatgctggct ttgtccgctc attcgtcgcc gtgaacctca cgttgccgga
    12121 cttgttctgg ggttaatcct cattcttggc ggtgagatga tcgggcgtgt gctcttttat
    12181 ggcctgcaca tgacggtagg aatggctatt gcaggttaac aaattggtgc ggggcaaccc
    12241 gcacatttca ggatgttagg aatgacccat ttttcacagc aagataattt ttctgttgcg
    12301 gcgcgcgtgt tgggcgcgct gttttattac gctccagaga gtgcggaagc cgcacctctg
    12361 gttgcggtac tcaccaatga cagctgggaa actcagtggc ctctaccaga agcgtcatta
    12421 gcgccgctgg tgacggcttt tcagacacag agtgaagaga ctcacgccca agcctggcag
    12481 cgtttgtttg tcggcccgtg ggcactgccg tctccgccgt ggggatcggt ctggctggat
    12541 cgcgaatctg tgctgtttgg cgattcaaca ttggcacttc gtcagtggat gcgcgagaaa
    12601 ggcattcagt tcgaaatgaa gcaaaacgaa ccggaagatc attttggttc actgctgctg
    12661 atggctgcgt ggttggcgga gaatggtcgc cagacagaat gcgaagaatt gctggcatgg
    12721 cacctttttc cgtggtcaac acgttttctt gacgttttta tcgaaaaagc agaacaccct
    12781 ttctaccgtg cactgggtga actcgctcgc ttaacgctgg cgcaatggca atcacaactg
    12841 ttgattcctg tcgcggttaa accgctgttt cgataggatc accgtattat tgccggatgg
    12901 tgacgattac acgcaattcg gcaattaata ggttacagtg tcacgttttt ttatctctta
    12961 aagcacgcac tgcttttgcg gctggcctct tttgccgcaa aatagtcgcc cgtgtttcat
    13021 tgcccatttc tgctcatgca tcatctacac atctatccgg atctgcgcac tatgttccgc
    13081 cgtctgctta tcgcaacaat cgtcggtatt cttgcggcct ttgccgttgc cgggtttcgt
    13141 catgcgatgc tgctactgga gtggctgttc ctcaataatg actccggcag tctggtcaat
    13201 gcagcgacaa atctttcccc gtggcgacgg ctgctaaccc cggcgctcgg cggactggcg
    13261 gcgggtttgt tgctgatggg ctggcagaaa tttacccagc aacgccctca tgcgccgacc
    13321 gattacatgg aagcgttgca aaccgatggt cagtttgatt acgcagcaag cctggttaaa
    13381 tcgcttgcct ctctactggt agtaaccagc ggcagtgcaa ttggtcgcga aggtgcgatg
    13441 attcttttag ctgcccttgc agcctcgtgt tttgcccaac gttttacgcc acgccaggag
    13501 tggaaattat ggatcgcctg tggagctgcg gcgggaatgg ctgcggccta tcgcgccccg
    13561 cttgctggca gtttatttat agccgaagtg ctgtttggca ctatgatgtt ggcctctctc
    13621 ggcccggtga ttatttccgc cgtcgtggcg ttgctgatta gcaatcttat taatcatagc
    13681 gacgcattac tctacagcgt acaactctca gtgacggttc aggctcgtga ctatgcgctg
    13741 attatcagta caggtgtgct ggcaggtctg tgcggaccac tgttgttaac gttaatgaac
    13801 gcctgtcatc gtggatttgt gagtctcaag cttgcgccgc cctggcaact ggcactgggc
    13861 gggttgatcg tgggtctgct ttccctgttc acacctgcag tgtggggcaa cggctatagc
    13921 accgtacaat cctttttaac cgctccgcca ctgttaatga tcattgccgg gatcttcctc
    13981 tgtaaactgt gtgccgtgct ggcgagtagt ggttctggcg cgcccggtgg tgtctttaca
    14041 ccgacgctat ttatcggtct tgccattggc atgttgtatg gtcgtagcct gggattatgg
    14101 ttacctgatg gcgaagaaat tacgctttta ctcggattga cagggatggc gacactgttg
    14161 gcggcgacca cgcacgcgcc gattatgtcg acgttgatga tatgtgaaat gaccggggag
    14221 tatcagctac tccccggttt attgattgcc tgcgtaattg cgtcggtaat ttcgcggacg
    14281 ttacaccgtg actctatcta ccgccagcac actgcgaagc atagctaagc ggatgtattg
    14341 ccccagttca cgctgttcgg cgcgcggcag ataaggcagt tcaccaatga gcggtgccgg
    14401 aagtttttta ccgagcacat caatgatttc cgcataatgc gccagtcctg ggttgattcg
    14461 gttagccacc cagccaatga gcggcagccc gtcgttggca atcgcctgag ctgttagcag
    14521 tgcatggtta atgcaacctt cctgaatacc gacaaccatc aacaccggca gttgttcctg
    14581 caccacccat tcagagagcg gacgcaaatc attcatcaga ctgcgccagc cgccagtccc
    14641 ttctaccacg acatgatcga ctttatcggt caggtttgcc aggccgtttg aaatgagggt
    14701 gtaattgatt gggcaactgt gcgccacgct actttcttct tcgcttaacg cgataggatt
    14761 aactgcttca taaggcagtt cgatggttga aacactctgc aacaccaggg catctttatt
    14821 acgcagccct tcgggtgtct ctttgctccc cttcgctacg ggtttatatc ccgcaaccgt
    14881 ttttccctgg gaggctaacg cttgtagcaa tgcgcgggaa accaccgttt tccctacaga
    14941 agtgtctgta ccggtaataa agaaacgctt cagcatcact aactccaccg ttatgcttca
    15001 caaatataaa ccaggaaaat aattaacctt gaaagtctaa gttatgcttt cctggcccaa
    15061 attgagatag cgcaaatttt ggtagaacag ttaaaaaatg ttaaccctgc aacagacgaa
    15121 tcaacaaaga accgttatac atcgcgtctt ttaccagcgc agcgcctgcc atcgtgccct
    15181 ggttagaaaa ctgagtactc tcaacgctaa tgtgctgact atacgcagga agggcctgct
    15241 gacggatgct gtccgagatg actgggaaga ggatatctgc cgctttactt aacggtgagc
    15301 caatcagtat tttttgtggg ttaaataaat tcaccatgat ggcaagaatg cgaccgacat
    15361 gcgcgcctac cccggtaatg atgtcttttg ccagtagatc gccgcgcaat gccgcctgac
    15421 acaatgagtc cacggttaac ggttgtccat gtaacatcga gctcatggat tgattgagac
    15481 gcagctgtgc cagctcaaga atactgtcca cactggcgat ggtttcgagg caaccgtgat
    15541 tcccgcaata acagcgtttc ccatacgggt cgacctgtgt gtggcctatt tccacgaggc
    15601 tactgctgcc tgcgtgtagc agatgaccat cagtaatgac gcccgccccc acgttgtgat
    15661 cgataaccac ctgaatcaca tcgcgcgccc cgcgtgaggc accaaacaag gcctcagcca
    15721 tcgtccatgc gctgatatca tgctgaatat aaaccggtac gccggtatgc tgctccagcg
    15781 cctcgccgag cggcatctct tttacatcct cgtagaacgg catgcgatgt acaataccat
    15841 tttccgtatc aataattccc ggcaaggtta tggcaatcga agtcagacgc tcaagttttt
    15901 tctggtggcg gataaaaaac tgatcgatat gggaaatgat acgctccagc aatggcgagt
    15961 catcttttaa cgccagttcc tgcgactcct ccactaccag tttgctgctc agatcgcgca
    16021 aagcaaggaa aatctccccg cgactaatgc gcagagaaag atagtgccag gcttcagttt
    16081 caaccaccag ccccactgcc ggacggccac ggttccccgc ttctttgatt tccagctctt
    16141 gcaccaggtg tgcttcgagc atctcacgga caattttagt gatactggca ggagccagtt
    16201 gcgccagacg ggaaagatcg atacgcgaga ctggaccaag ctgatcaatc aggcgataaa
    16261 ccgcgcccgc gttggtctgc tttatttgat caatgtgccc aggctgattt tcagcaacca
    16321 ccgcatactc cctatatttt cgcgctccga aataatctgt aggctatggt gaagcacttc
    16381 aatacgtgtc gtcaaatttt tacttaggca tgtgattaac agcacatttt tcgggctttt
    16441 tcgctgaaat ttccctcacc tgagagcatt cagcagatga atacgaaagt tacgcgcagc
    16501 cggactttgt tcatgatgtt tcggccagac caaccacatt tcagaaaccg catcctcttc
    16561 agcaatcggc acccagcgca tttcgttgag ctgaacacgt ttaaatgacg caggcaaaat
    16621 tgaaacaccc agaccagcgg aaaccagacc gatgatggtc attgcctcac ccacctcctg
    16681 agtgatgacg ggcgtcaaat tgtaacgtct catcagcccg agaatatcgt catacagccc
    16741 tgttccgacg tgcggatcaa aaaagacaaa gggttctttc gccagttcag ccagcgttac
    16801 actcgggtta tttgccaggg gatgatcgtg cgggatcatc gccataagcg gttcatggac
    16861 gatgactgcg tgttcaagcg tctccggtaa cgctgtgtta cgcagcaatc ccatatccag
    16921 cgttccttca atgagcggag cgatttgctc gcgagtgttc atttcgcggg tttgtaaatg
    16981 gacatcggga taatcacggc gaaacagcga taacgtatcg gacaccgccc gaataaaagg
    17041 agcggacgaa gtaaaaccaa tgcgcaactc ccccgcttcg ccctgatgca gcctttcagc
    17101 gcgagcggca gcatcatcca ccatagacag gatttgccga ctatctacaa gaaactgttt
    17161 tcctgcttcc gtcagcaata cactgcgatt ggtccgtgcc agcagtcggg caccaatttg
    17221 ttgctccagc gcctgaatct gctgacttag cggtggttgc gaaatattca ggcgggcagc
    17281 ggcacgcccg aaatgcagct cttctgcaac agcgacaaag taacgcagat gacgaagttc
    17341 aatattcata tttaaaacat cttatttgag attattaata tattagacag aacaattcga
    17401 ttttcctaat ctattcataa gcctgatcca caggcatatt tagcaaggat ttcaagtgag
    17461 ccgtactaca actgttgatg gcgctccggc aagcgacact gacaagcaaa gcatttctca
    17521 gccaaatcaa tttattaaac gcggtactcc gcaatttatg cgcgtcaccc tggcgctttt
    17581 ctctgccgga ctggcaacct tcgcacttct ctattgtgtg caacctatcc ttccggtgct
    17641 ttcgcaggag tttggcttaa cacccgcgaa cagtagtatt tcactgtcca tttcaacggc
    17701 gatgttggct atcggtttgc tgtttactgg cccgctatcc gatgccattg gtcgcaaacc
    17761 agtgatggtc acggcgctac tgttggcctc catttgtacg ttactttcga caatgatgac
    17821 cagctggcac ggcattttga ttatgcgcgc cttgattggg ctttcgttaa gtggggtggc
    17881 agccgttggc atgacttatc ttagcgagga aatccacccc agtttcgtgg ccttttcaat
    17941 ggggttgtat atcagcggca actcaattgg cggcatgagc ggacgcttaa ttagcggtgt
    18001 cttcacggac ttttttaact ggcgaattgc tctggcggca atcggttgtt tcgcgttggc
    18061 ctcggcgttg atgttctgga aaatcctccc tgaatcacgc cattttcgcc cgacttcgct
    18121 gcgccctaag acgttattca tcaactttcg tctgcactgg cgtgaccggg gattaccgtt
    18181 attgttcgca gaaggctttt tgctgatggg gtcgttcgtc acgctgttta attacatcgg
    18241 ctataggttg atgctttccc cctggcatgt cagtcaggcc gtggttggct tattatcgct
    18301 ggcttatttg accggtacat ggagctcacc caaagccgga accatgacca cccgctatgg
    18361 acgcggtccg gtgatgttgt tttcgacggg ggttatgctg tttggtttac tgatgacctt
    18421 attcagctcg ctgtggctga tctttgccgg aatgttactc ttctcagccg gattcttcgc
    18481 agcccactcc gttgccagca gctggatcgg cccccgcgca aaacgcgcta aaggccaggc
    18541 ctcctcgctg tatctgttca gttactatct ggggtcgagt attgccggga cgctgggtgg
    18601 tgttttctgg cataactatg gctggaacgg cgtcggcgca tttattgctc tgatgctggt
    18661 cattgctctg ctggtcggga cgcgtttgca tcatcgtctg cacgcctgaa aaaataagtc
    18721 cggactgagg taaatacccg tccggactta ttgcctgctc aaaccaacgt taatagccat
    18781 cctaaaatag acgaagcgcc agccaattcc cgcagcgcat ctagcgtcat caggattata
    18841 agtacccaaa taaacggatt cattttgctg tgtgtcattt attactgatg cgcagttatt
    18901 ctaccgcttt gtaagtagta aaatagttaa cacgatcaag actactatta ttggtagcta
    18961 aattttcctt aagtcacaat acgttattat caatgctgta atttattcag cgtttgtaca
    19021 tatcgttaca cgctgaaacc aaccactcac ggaagtctgc cattcccagg gatatagtta
    19081 tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa
    19141 aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgctgcc tttgctgcag
    19201 agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgcct gcgaaaacca
    19261 cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagc
    19321 atcataaaaa tgccaaagct gaacagaaag cccctgaaca aaaagcgcag gcggcgaaga
    19381 aacacgccaa gaaacacagc catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt
    19441 aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaagcgg
    19501 gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt
    19561 cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgatc
    19621 gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt
    19681 tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtacaacca ttgctgtagt
    19741 gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccaa
    19801 atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa
    19861 tgacacgacc caatcaccgt gggatgcggt tggccaactg gaaacggcca gcggcaattt
    19921 atgtacggcg acactgattg cacccaatct ggcattaacg gcaggacact gtttattgac
    19981 gccgccaaag ggtaaagccg ataaagcggt agcgctgcgt ttcgtgtcaa ataaaggtct
    20041 ttggcgctat gagatccacg acatagaagg ccgcgttgat ccgacactgg gaaagcggtt
    20101 aaaagcagat ggggatggct ggattgtacc tcccgcagct gcgccctggg acttcggttt
    20161 gattgtgcta cgtaatcccc cttctggcat tacaccgttg ccgttatttg agggagataa
    20221 agccgcgctt accgccgcat taaaagcggc tggtcgtaaa gtgactcagg cgggctaccc
    20281 tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt gaagtgactg gctgggcgca
    20341 aacgtcggtg atgtcgcatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat
    20401 gttgcatacc gatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa
    20461 agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga
    20521 tcaactgtcg caaaaataat gttcaaacgc tgcccgacag tgcgggcagc gtgttcatca
    20581 ggcaagtttc accatgatca ttccagccaa cagcaagacc aggccaatcc agcctttacg
    20641 atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc
    20701 gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag aaagcgcact
    20761 aaaggcagcc agcaccgccg ccagggagag caagccaaat attttgcaac gaaagccgtc
    20821 agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaagc
    20881 ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta cgcgctttac
    20941 gggtacctga cttgatcaac acaatcccgg ccaccagggt ggttaacccg gcaattttca
    21001 tcagcgataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga
    21061 taccttccca cagcgcataa gctacgccta aggcgatttt tttaacggcg aaagagagaa
    21121 atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga
    21181 cgctcgccca tttcattgac agcgtaccgg taatttctgt agcaatagcc agacctaata
    21241 aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat tttcgcttaa
    21301 ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgctta gcgcaagctt tggcagaaga
    21361 aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt ggatgaggta
    21421 cgaagatggg taaatgtaga aaataacgtc ctgaacaaat tgtccataat attacaatta
    21481 tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctcggtagtt gaacatgcct
    21541 gatttgtatc atagcttaag aattaactca aaatattttc acttctttac ctgagcggtt
    21601 tgattttcgt tatgatgatg gagcgaaaaa gacattatta ttagcaaaga agaaaaaacg
    21661 gggacaagca tggcaaagcc gatcatcacg ctcaatggcc taaaaatcgt cattatgttg
    21721 ggaatgctgg tcattattct ctgcggtatc cgttttgccg ccgagatcat cgtgccgttt
    21781 attctcgcat tatttattgc tgttattctt aacccgctgg tgcaacacat ggtccgctgg
    21841 cgtgtgccgc gtgtactggc agtgtcaatt ttgatgacca tcatcgtgat ggcgatggtg
    21901 ttgctgttag cttatctggg ttccgcgctc aatgagttga cgcggacgtt accgcaatat
    21961 cgcaactcta ttatgacgcc gctgcaagcg attgaaccgt tgttgcaacg cgtagggatt
    22021 gatgtctcag ttgaccaact ggcgcattac attgatccga acgcggcgat gacgttgctc
    22081 accaacttat tgacgcagtt atctaatgcc atgtcatcaa tctttttatt gctgctgacg
    22141 gtgctgttta tgttgctcga agtgccacaa ttgcccggaa aatttcagca aatgatggcg
    22201 cgtccggttg aagggatggc ggcgattcag cgtgcgattg acagtgtgtc tcattatctg
    22261 gtgctgaaaa cagccatcag catcatcacc ggcctggtcg cctgggcgat gctcgccgca
    22321 ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct ttgcgcttaa ttacatcccg
    22381 aatattggtt cagttctcgc ggcaatcccc cctatcgctc aggtactggt gtttaatggc
    22441 ttctacgaag cgttgctggt gctggcggga tatctgctga ttaatctggt cttcggcaat
    22501 attctggagc cacgcatcat ggggcgtggg ctggggcttt ccacattggt ggtatttttg
    22561 tcgttgattt tttggggatg gttgttagga ccggtgggta tgctgctttc cgtgccgttg
    22621 acaattattg tcaaaattgc gcttgaacaa acagcgggag gtcaaagcat cgccgttctg
    22681 ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc cgtcagggtt acagagcttt
    22741 caggattgca tccacgctgg ctttggcgtc accaaacagc atgtgggtgt tttccttgaa
    22801 gaagagtggg ttttgcacac cagcatagcc agtgttcatc gaacgtttaa agacaatcac
    22861 gttctgcgct ttccacactt ccagcacagg cataccagca atcggactct tcggatcatc
    22921 ctgcgccgcc gggttaaccg tgtcgttagc accaatcacc agtacggtat cggtatcagc
    22981 aaagtcatcg ttgatctcgt ccatttccag cacgatgtca tacggtactt ttgcttcagc
    23041 cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc gggtggatac cgaaacgcac
    23101 gttaatacca cgagcacgca atttctcagt aatttcagcg acaggatatt gcgcctgcgc
    23161 aactgccatg ccgtaccccg gagtaatgat cactgaatgg gagtttttca gcagttccgc
    23221 tgtctcttct gcggtgattt cgcggtgctc gcccacttcc tgatcatcac cagtagaaga
    23281 gccgtcggtg ccgaaaccac ccgcaataac gctgataaag gaacggttca tcgccttaca
    23341 cataatgtaa gaaaggatag cccccgaaga accgaccagc gcaccggtca caatcagcag
    23401 gtcgttgcta agcataaagc ccgcagccgc agccgcccag ccggagtacg agttcagcat
    23461 cgacaccacc actggcatat ctgcaccacc gatggaggcg actaaatgcc agccgaatac
    23521 cagcgcaatg gcggtcatta tcagcaatgc cagcacttgc aggccgacgc tgtccgtgcg
    23581 aacaaataca atcagcagca ggaaggaaac gaccagagcc gccaggttca ttttgtgacg
    23641 gtttggcagc atcaacggtt tagacgaaat cttgccacac agtttgccaa acgccaccac
    23701 cgaacccgta aacgttaccg ccccgatgaa gataccgagg aacacttccg tcaggtgaat
    23761 attgaccaga atcggtgcca ttcccgcgtc atgatgcaga tagctgttaa aaccaaccag
    23821 cactgccgcc agacccacga agctatgcag gatcgccacc agttctggca tttcggtcat
    23881 ttcaactttc ttcgccagac ggataccaat tgccccacca ataaccatcg ccagcaagat
    23941 ccagccaaca ttacccgtat ccggtccaaa aatggttgcg attaacgcaa tcgccatacc
    24001 ggcgataccg aaattgttac cctggcgaga cgtttcatgt ttcgaaagcc cggccagact
    24061 gaagataaac aggatcgcgg caacaatgta tgcagctgta actaatcctc cagacatatg
    24121 ttacccctta atttttgcgg aacattttca gcatgcgctg agtcacggtg aagccaccga
    24181 aaatattaat gctggctata agcaccgcga taaaactaag gaagctaacc cagccgccct
    24241 ggccaatctg caacagtgct ccgacaacaa taatccctga aatcgcgttg gtgaccgaca
    24301 tcaacggtgt atgcagcgcg tgcgatacat tccacaccac gtaataaccg acaacgcagg
    24361 ccagcgcgaa cacagtgaag tgtccaagga actcttttgg cgcaacgctt gccatccagc
    24421 cgaaaaggat gattgccaac gccatcaacg cgtatttacg ccacggtgag caggcacatt
    24481 tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc ctgcggctga gctgatacct
    24541 gaatcggcgg tgccggccag gtaatttcgc ccgcacggat cacggtcacg ccgcgaatca
    24601 ccacatcatc aaaatcaaca gtgatgttgc cgtctttctc tttgcacaac agtttcagca
    24661 gattaacgag gttagtgccg taaagctgtg aggattgcgt cggcagacgg cccggaagat
    24721 cggtgtaacc aattactttg acaccatttt ccgtagtgaa gatttcaccc ggcacggtgt
    24781 attcacagtt gccgccgttt tgggctgcca ggtcgacaat cacactgccc gccttcatgg
    24841 agtcaaccat ttcacgggta attagcttcg gcgctggttt gcctggaata agcgcggtgg
    24901 tgacaatgat atcgacctct tttgcctggg cggcaaagag ttccatttcc gctttgatga
    24961 acgcgtccga catcactttg gcatagccat cgccgctgcc cgcttcctct ttaaaatcca
    25021 gctcgaggaa ttctgcgccc atactttgaa cttgttcttt cacttccggg cgggtgtcga
    25081 atgcacgcac aatcgcgccg agactgtttg ctgcgccaat ggcagccaga cctgcgacac
    25141 cggcaccaat caccatcact tttgccggtg gcactttccc ggccgcagtg atttgtccgg
    25201 taaagaagcg cccaaattca tgtgccgctt caacaatggc gcgataaccg gcgatattcg
    25261 ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat acgcggcaca gagtccatcg
    25321 ccatcacggt cacattacgc tccgcaagtt tttgcattaa ttccggattc tgcgcaggcc
    25381 agataaaact caccagcgtt gtcccaggat tcagtaacgc aatttcatca tctaacggcg
    25441 cattgacctt cagaatgatc tctgactgcc agacgctatt cccttctaca atttcagcgc
    25501 ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg acccgcgccg ctctctaccg
    25561 cgacggtaaa acccagtttc agcagctgtt ccactgtttt tggcgttgct gcaacacggg
    25621 tttcattggt taaccgttct cttggtatgc caattcgcat gatattccct tccatcggtt
    25681 ttattgatga tggtttgctt gtgtcaggag ccacacaagc cgctcatgta cgagctaaat
    25741 gttactccgt taaaataaat tagtaacaaa cgccctataa cgtactgaaa attatgcctg
    25801 tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc aaaatattaa cgattcagcc
    25861 gtgatagtgg gataaacacc ttagaacgcc ggataaagac tgataattgt cttcggcggc
    25921 cgggtaaaac gagacatcgc cccggtacaa atcactactt aacattaaat taacttatac
    25981 aattcagttg cttcagtagt attgatggtg atacggctgt tttttaagca tagacggtca
    26041 tttgagcaga attaaaattg gtttaaggaa tgtgatatga aaaatgacgc agacagttac
    26101 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
    26161 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
    26221 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
    26281 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
    26341 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
    26401 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
    26461 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
    26521 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
    26581 agccagaagt caatgatgcc atcaccaaag cggcaaaagc gaaaggtgcc tactctttct
    26641 acattgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
    26701 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtcatcccg gcagattccg
    26761 aagcaggacg tgcagctctg gctgccggtg gcgaagctgc gaagaaagtt gagatcccgg
    26821 gggttgcgac taccgcatca ccaagttctg aagtcggtcg cttctttgaa acccagtcat
    26881 caaaaggcgg gcgttacacc gtcacgctcc cggatggcac taaagtcgaa gaactgaaca
    26941 aagcgaccgc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
    27001 acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
    27061 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt
    27121 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
    27181 cacaaatttc cccgcgatca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
    27241 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
    27301 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
    27361 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
    27421 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
    27481 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
    27541 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt
    27601 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
    27661 gccggaattg cgcctgtttg gcgatggtaa tacctggcaa tcgatcgtcg gtgcatcgac
    27721 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
    27781 cctggtggcg acactggcaa aactgttgcc gctgggtctg tttgttgtac tggcgatgat
    27841 gatgttcaaa ctggatacct tcaagctcga cttcaccgga cttgcacttg gcgtacccgt
    27901 gtgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
    27961 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
    28021 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggtgtggt
    28081 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
    28141 gatgggacca tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
    28201 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggccgcca ctcataaagc
    28261 attcccgcgc atttttgcgc gccagaacgc acaagctgcg ccatccgctt cactgtggct
    28321 gaccaacatc tgcgtgcaaa tttgtctggt gctcatctgg ctgaccggtt ccgattacaa
    28381 cacattgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
    28441 cttgctaaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
    28501 tggcttatgg ttattgtacg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
    28561 tgcaccgggg ctgctggttt tcctgtacgc gcgtaaaacg catacccatg ataatgtact
    28621 gaatcgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
    28681 gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa
    28741 agcccaaccc ttgccaatat taattactgg cggtggtcgt cgcatcggcc tcgccctcgc
    28801 ctggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacgc actatcccgc
    28861 cattgatggg ctgatgaatg cgggtgcgca gtgtattcag gctgattttt cgaccaacga
    28921 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
    28981 gcataacgcc agcgcgtgga tggcggaaaa accgggtgcg ccactgaccg acgtactggc
    29041 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgctc tggaaagatt
    29101 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
    29161 gcgcggtagc gacaaacata ttgcctatgc tgcaagcaaa gcggcactgg ataatatgac
    29221 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
    29281 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact
    29341 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
    29401 ctgctttgtc accggacgca gtctcccact tgatggcggt cgtcatctgc gttaatgcag
    29461 cttaatccag catataatca acacccatgc gcttattccc cagcacgcaa cagagccaac
    29521 aaaagcagcg ggcaagcgca tcattccggt gaaataccat agggacgcca ggtaaacaaa
    29581 atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg
    29641 ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc
    29701 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
    29761 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
    29821 agtagaatac ctgatgaaac cttgtttaga aacgattgat agtaagtaaa aacagtgcgg
    29881 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
    29941 atgatgcgga agtcggtgca ctgattgccg catacctggc aaaacatgat atgcaggtta
    30001 ccgtagagcc gcgcggcgac cgggccgaag aaactattct gcgagaaaat ccggatttgg
    30061 tgttactcga catcatgcta ccaggcaagg acggcatgac catttgtcgt gatttacgcg
    30121 caaagtggtc cggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
    30181 tggcactgga aatgggcgcc tgcgactata ttctcaaaac gacgccccct gctgttttgc
    30241 tagcgcgttt acgcttgcat ttgcgtcaga atgagcaagc cacagtgacc aaaggtattc
    30301 aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccca
    30361 tcaaccgcgt agtctccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
    30421 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
    30481 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
    30541 taagaaaaaa actgctcgat aacgccgcag aaccttatcg tattaaaacc gtgcgtaaca
    30601 aaggctatct ttttgcgcct catgcgtggg aataagcgat gaaaaaactg tttatccagt
    30661 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggtcggg ctggtgtaca
    30721 aatttaccgc cgaacgcgca ggcaaacagt cgctggatga tttgatgaac agttcgctgt
    30781 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag
    30841 agatggattt aaatctctct ttcgatctgc gtgtcgagcc gctgagtaaa tatcatcttg
    30901 atgatatttc catgcaccgg ctgcgtggcg gcgaaattgt cgctctggac gatcagtaca
    30961 cttttttgca acgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
    31021 tttattacct ccatcagatg cgattgctgg atatcgccct gatcgctttt attgccattt
    31081 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
    31141 aagcagcggc gcaacgattt ggcgatgggc atctcagtga gcgtatccac tttgatgaag
    31201 gttcgagctt tgaacgactt ggcgtcgcat ttaaccagat ggcggacaat atcaacgcct
    31261 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
    31321 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
    31381 tgaatcgcga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
    31441 tcgatcgccc acaaaatgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
    31501 cgcatctggc agatattcag gcagtaacgc ccgataaaac ggtacggata aaaacgctca
    31561 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
    31621 tcaataacgc cctgcgctac tgtcattcaa cggttgaaac cagcctgcta ctgtcgggga
    31681 atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac
    31741 atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
    31801 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
    31861 acaccagcga actgggtggt gcccgcttct cgttcagctg gccgttatgg cataacatcc
    31921 cgcaatttac ctctgcctga cactatgcgc acggtggtca agtcaccacc attgtgctat
    31981 aaaataagga tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
    32041 gactcaacac tacttttcgc cagatggaac aagagctggc tgcatttgcc gctcatcttg
    32101 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
    32161 agcataatcc gcttaatcgt attgaggtaa aacaacatct cggcaacgac gcgcagtcgc
    32221 tggcgttgcg tcattttcgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
    32281 aggccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacttt tcgcaatcag
    32341 cgttagtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
    32401 cggttgaatc agaactcccc tccgcggcac gttttgaatg ggtgcatcgt catttgccgg
    32461 ggctgattac acttaatgct taccgcacgc tcaccgttct gcacgacccc gccactttac
    32521 gttttggctg ggctaataaa catatcatta aaaatttgca tcgtgatgaa gtcctggcac
    32581 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
    32641 aaagaaaact ggagcgagaa tatcaggata tcgctgccct gccacagaac gcgaagttaa
    32701 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
    32761 aacaagtaca acacgcctgc cctacgccgc tgattgcact gattaatagg gataatggcg
    32821 cgggcgtgcc ggacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt
    32881 ataaaccaca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
    32941 attaaccccc ggctttcata ctgtcgacca tctgttctgg ccgtacccag cggtcaaact
    33001 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
    33061 gcgctttttt ggcgatctcg gcggctttgt cataaccaat gtgggtgtta agcgcagtca
    33121 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaatgccca
    33181 ctgcgcagtg tttgttgaaa ctttccatgc catctgccag caagcgcacc gattgcagga
    33241 aattatggat caccatcgga cggaagacgt tcagttcaaa gttaccggaa gcgccaccca
    33301 tgttgatcgc cacgtcgttc cccatcacct gacagcagag catggttaat gcttcgcact
    33361 gtgttgggtt cactttccct ggcatgattg agctgcccgg ctcattttcc gggattgaga
    33421 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcaattttca
    33481 tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg
    33541 ccagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
    33601 catctgctac gcgacgcgca tattccggat gggtatttag tccagtaccc accgctgtac
    33661 cgcccagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct
    33721 ccagcatcgc tacccagccg gaaatctcct gccccagcgt taacggcgtg gcgtcctgca
    33781 agtgggtacg accgattttg acgatatcag caaatgcgcg ggatttttca ctcagcgtct
    33841 gggtcagggt tttaagttgt ggaatgagtt gcttgcgcag cgccagtagt gccgcaacgt
    33901 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
    33961 gaactttacg ttccatcccg cgcacgccac cgagtaattc actggcccgg ttagccagca
    34021 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
    34081 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
    34141 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
    34201 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
    34261 agcgctgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgct cccatcgaat
    34321 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgattaattt tttctttctg
    34381 ttttgctttc gttaagcaac ttttagctca ctcattattt accatttgat aacaaacgtt
    34441 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
    34501 cgggtgcatt gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
    34561 ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc tagttccgga
    34621 tattcaacac attccaggct cttaatgctt ccctgcgcca atatagctgc cggaccaccg
    34681 atactaccga ggtagaaacc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
    34741 ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa
    34801 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
    34861 gccggacccg cgtagtaaat cggatgatct ttaatgtact gcggcagccc ttcaccgtta
    34921 tccatccgct ctttcagttt ggcgtgagca atatcacgac cgacgataat cgtgccgtta
    34981 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
    35041 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tacgcagctc ttccgggata
    35101 tatttgcctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
    35161 ttgatattac ggtcagcaga gcaggagacg cccataccga ccgggcagga tgcgccgtga
    35221 cgtggcaggc gaatcacgcg gatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc
    35281 agaccaagat tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaacgcc
    35341 tgaccgtgct cattcccttc cgttggcagt tcgtcatagt atttagcgga agccagtttc
    35401 accgttttaa ggttcgtttc cgcagaagtt ccaccaatta cgaacgcaat atgatacgga
    35461 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc
    35521 ggcgttagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
    35581 ttggcgatac agaggaattt gtactcgtcg ccatcaacgg cataaagatc gatctgcgct
    35641 ggcaagttgg tgccggtatt cacctcttta tacatatcca gcggcgcgtt ttgcgagtag
    35701 cgcagattat cttcgatata agtgttatag acaccgcgtg ccagcgccgc ttcatcaccg
    35761 ccaccggtcc atacacgctg cccttttttg ccaacaataa tcgcggtgcc ggtatcctga
    35821 caggttggca gaacgccttt cgccgcgatg tcggagttgc gcaggaattg cagcgccaca
    35881 tatttatcat tttcgctggc ttccgggtca cgcagaatgt cggccacctg ttgttggtga
    35941 gccggacgca gcataaatga cgcatcatga aacgcctgac gcgccaacaa agttaacgct
    36001 tcgggtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg
    36061 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    36121 ggtttgtttg acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt
    36181 tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag
    36241 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagcgg
    36301 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    36361 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca
    36421 gccagccgat ggccgagctg tggatgggcg cacatccaaa aagcagttca cgagtgcaga
    36481 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtaataaa tcgactctgc
    36541 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    36601 cagcacagcc gctctccatt caggttcatc caaacaaacg caattctgaa atcggttttg
    36661 ccaaagaaaa tgccgcaggt attccgatgg atgccgccga gcgtaactat aaagatccta
    36721 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    36781 aattttccga gattgtctcc ctactccagc cagtcgcagg tgcacatccg gcgattgctc
    36841 actttttaca acagcctgat gccgaacgtt taagcgaact gttcgccagc ctgctgaata
    36901 tgcaggggga agaaaaatcc cgcgcgctgg cgattttaaa atcggccctt gacagccagc
    36961 agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggat gacagcggtc
    37021 tgttctcccc gctgttgctg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt
    37081 tcgctgaaac gccgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcgaactccg
    37141 ataacgtgct gcgtgcgggt ctgacgccta aatatattga tattccggaa ctggttgcca
    37201 atgtgaagtt cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggtg
    37261 cagaactgga cttcccgatt cctgttgatg attttgcctt ctcgctgcat gaccttagtg
    37321 ataaagaaac caccatcagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg
    37381 caacgctgtg taaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    37441 ccgccaacga atcaccggtg actgtcaaag gccacggccg tttagcgcgt gtttacaaca
    37501 agatgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    37561 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    37621 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    37681 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    37741 aaactgacag ctcctgaatc caacctggaa gtcagttatc aaaactatca tcgcggcgta
    37801 ttcagcagcc agttgcaact gttggtgaaa cccattgccg gaaaagtaaa tccgtggatt
    37861 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccgcttgcc
    37921 cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat
    37981 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    38041 tcccgcattg gttacagcgg agattccagt tccgacattt cgctcaagcc actgaattac
    38101 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgcagacaga
    38161 gacggcaaag ctatctccct ttccggggag gcgcaaagtg gtcggataga cgcggttaac
    38221 gaatacaacc agaaagtgca gttaaccttt aataatctga aaaccgacgg ttccagcacg
    38281 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cgctggaaaa aatgaccatt
    38341 tcagtggagg gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat
    38401 ctggtcaatg acggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    38461 gtacagaatc aggatctggg cagtggcaag ctgactttaa aagtcggcca aattgatggc
    38521 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    38581 ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc
    38641 gctctgccgc tgatgctgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa
    38701 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgacg
    38761 aaagaagcgc cgcaaacgct ggcgcaggaa gtagaccgct cggttaaatc tctggatgcg
    38821 aaactgacca ttccggtgga tatggcaact gagtttatga ctcaggtagc gaagctggaa
    38881 ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca
    38941 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcaa
    39001 tatgctaacg gtcagataac gttaaacggg cagaaaatgc cactggaaga tttcgtgggt
    39061 atgtttgcga tgccgacatt aaacgttccg gctgtacccg ctattccgca gcagtaattc
    39121 acagccaaaa cgccggagat ttttctctcc ggcgtttttt cttacttcag cataaagtca
    39181 tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg
    39241 cgcttttccc gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg
    39301 ctgacccgat agttcatacc ccctaagata cccgcgcctt caatccgcac atctcgttta
    39361 ctggattcat agccgcgggt cacaatcggt gcaaatgtca gcgtaaattg cggagttaaa
    39421 cgatagttga cgcccaattg ccaggactgc attccttcac ggttacgatc aagacttaat
    39481 gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa
    39541 ccaccactgc caaatacgct tccccacggc gcattccagg tcaattgtcc actaaccaac
    39601 gccgtttcat tgggctggct ggtattacga cttaaacctt caagttgcgc gtaaaagccg
    39661 agcaactccc ctttcaacgt ataattcggg tcaagcggta tgcttttctc gagaatactt
    39721 tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca
    39781 tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg
    39841 ctgttataaa ccccttcatc acgaataccg gttgttgaat cggtccataa aaatactcgt
    39901 gaacgccaca ttccccacag atcaatattt ggcgttaaag cccaataacc taatgctcct
    39961 tcagaaacag tcggtaatgt acgttgcgaa ctgccagcca ttaatggaat acgataaggt
    40021 aagctaccat aactataatc cgtcccgaag cgacctaatt ttagcaggct attttcactg
    40081 ggttttattt ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac
    40141 cgggtactca tatcagcacg agcacctaat ttataaacat aatacacccc accttcaacg
    40201 ccaataatat tggaataata accactattg aaatcgagca atcctccctg tacccatgcg
    40261 taaatatctc ttcccgtgcc agcactgggc ttatcagccc tgcgcaattc gtttttcagt
    40321 ctgatgcgta gtcccgcttc atcgtcagcc agttgcgccg cgtaagcaga ggtaaggcca
    40381 gaggcagccg tcaggcaaat aacggccatg gccactattt ttctcatagt tgcacctttg
    40441 atgttctgat tatttttatt gaatattaac tagtgatatc gttgattaat tgctgctgaa
    40501 cttttttacg attatcaatt tcaacaacga tttctttgaa ttttttatcc gtgagcggat
    40561 aaaaccagat aatgacgaat gccagtagca taaatccgca aggtactaag gcaattgatg
    40621 tgcggatgcc cataataact tccggcgttt gcacctgatt ggcgatatat ccgcttaatc
    40681 ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc gcatttacgg gtaaatgaga
    40741 atagtgaata ggtgagccct tcaattcgca cgccggtcag gtattcaccg tattctacgg
    40801 tatcagcttc cagtgcccac atcacggtca tggtaacgcc ctggccaatt gatgcgatgg
    40861 ccaacgcaac caacgccacc ggcagtgacc agacggaaac ccagaagaac agcagataac
    40921 cgcaggttcc cagcaaagcg ccaatcagga aggtattctt tttaccgatc ctcgcgacca
    40981 tccccggcac cagcggtgcc gatgccacag taccgaccag gttttgcacc agtaccagca
    41041 cagtgaacag cccggtatca tttaacacat agcgcacgta gaacaacgac gaggcgctga
    41101 ccgcgaaggt cgaaatcagc acacacaccg caccgatgca caacataaac agcgggcgat
    41161 tccgtttcag ggtttgcaga ctgatcttca atgacggctg cgccacgata cgtaccacat
    41221 tctcacgcgt cgatttgaag cagatgaagt aaagcaccat tccggcaatc gccagcacaa
    41281 ttgtccagaa atgatatacc gacaccatct cttccgggct ggagttctta atgctcggtc
    41341 ctatcagaaa tgccaggcag acaaaggtca atgaagcggc aatcccgcga gccgcgccca
    41401 gacgggcgcg ggattgtggt tgttgggtca tcgcggtagc aagtgaacca taaggaatat
    41461 tcaccaggct gtagcaaagc ccgagtccca tgtaggtcaa atatgcatac acaactttgc
    41521 tgctatggct ccagtcggtc ggcacccaga ataccagcac gctgaagatc attaacggcg
    41581 cagtaccgaa gagtaaaaac gggcggaatt ttccccatcg ggtattcaca ctgtccacca
    41641 ctcgtccggc aaagacatcg gcgaaggcat cgaatacccg caccagtaac agcatggtgc
    41701 ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta actcaacagg aagagcgccc
    41761 ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct gtagccgacg atggtgcgcc
    41821 aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc
    41881 ggtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gggtgaagat
    41941 ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt
    42001 ccatacctgc tcaccgacga cagcgctgac gcgatcaaag acgcggtgat acatatccag
    42061 ccaggcgcac tgatactctt cactccacat gtcggtgtac attgagtgca ggccggctaa
    42121 cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag
    42181 aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccaacc
    42241 gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc
    42301 gcagaacatt acattgacac aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc
    42361 cgccagtggc gcaaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat
    42421 actccacatc accacgcttg ggtggttttt gtcacgcgca atcagctctt taatcgcctg
    42481 taagtgcgcc tgctgggttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt
    42541 gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagctg cagtttcatc
    42601 aatcaccacg attccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg
    42661 cgaggtacgg taggagttgg ccccaatcca gtccattagt gcgtgatcgt gcaccatcag
    42721 cacgttatcg aatcctttgc cgcgcaaatc cgcatcttca tgacgaccaa agccagtaaa
    42781 gtagaacggt ttgtggttga tcaggaactg ttcgcccttc actgccactg accggatacc
    42841 gacgcgcagc aggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata
    42901 gagataacct tcacccggtt gccagaggtg cggattcact acttgcaaag tcccgctggt
    42961 gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgacatcacc
    43021 atttgccccc acctgccagt caacagatgc gtggttacag tcttgcgcga catgcgtcac
    43081 cacggtgata tcgtccaccc aggtattcgg cgtggtgtag agcattacgc tgcgatggat
    43141 cccggcgtag ttaaagaaat catgaaagta agactgcttt ttcttgccgt tttcgtcggt
    43201 aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacagtgat
    43261 acgtacactt ttcccggcaa taacatacgg agtgacatcg gcttcaaatg gcgtatagcc
    43321 gccctgatgc tccatcactt cctgattatt tacccacact ttgccgtaat gagtgaccgc
    43381 atcgaaacgc agtacaatac gctggcccgc ccaacctttc ggtataaaga cttcgcgctg
    43441 ataccagacg ttgcccacat aattacgaat atctgcatcg gctaactgat cgttaaaact
    43501 gcccggcaca gcaattgccc ggctttcttg taacgcgctt tcccaccaac gctgatcaat
    43561 tccacagttt tcgcgatcca gactgaatgc ccacaggccg tcgagttttt tgatttcacg
    43621 ggttggggtt tctacaggac gtaacataag ggactcctca ttaagataaa aatactggtc
    43681 aacctttaat cttattagtt aactaattct gtgttgcaca cagttatctg tgagaaaccc
    43741 atggaagaga aatggaaata aaaagaactg acaccgcgat caaaaaacca aaaactgcaa
    43801 tacccacagc gtgatattga tcgcattaat ctttaaaaca gcgttgacag aagccacaaa
    43861 ataagcaacc gaacgttcgt tcgaacatat tgagaacgat aaactctcat gacatatggc
    43921 tacagtgaat attttggggg gaattctccg aacccgggaa ggataatttt tgcactgaga
    43981 aattacgata tgaaacgcgg gctcagttgt caccagcccg cggaaagaga gagaggatca
    44041 ggatgcggtt aagataccgc caatcatcgc gcgtaatccc tgagcaaacg acgcttcacg
    44101 cgccagatct tccgcttcga tatcggccag tacaccgtag gtcagatcca gcagtaaacg
    44161 tgaagtcatt gccgtgttga tgtctgggct tatctcgcct ttttgctggg catcacgcat
    44221 ccgctgggca acaaattccg tgatcgtcat atgtttattt ttaagcatgg cggcgacctg
    44281 tgggttacgc atcccttccg ccataatttc gaccaccagc gcccgttgtc caaaggcttc
    44341 atgggtgaga gagacaatag actcgaccat atagtcaacg aaatgaatcc cttcaatcgg
    44401 ttcccggaaa cgggccagcg ccctctcctg gtcctgtaag ataatcgcct gaatcagggc
    44461 ttctttggag atgaaatggt gatagagcgt cccgggacta atggcgcaag atttacagat
    44521 ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa atctctctgg cagcattgag
    44581 gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta tccatcataa aaccattata
    44641 gttagcgtga ataactagtt taccgcgttg tcaggtgagc gcctatcatt gctgttgaat
    44701 agtgcgaagg cacactctat tcgtatactt ttcactcgga aattggttaa ccaatagccg
    44761 taatttggct gccagttatt tagctgagac gttgatgagt ttatcccggt aaacaacaca
    44821 tacggctgtt taccggtccg ttagtgtatt aattgagctc ctgtacccca ccaccggaga
    44881 ccgtgagaat ttgtccgctt acccagctgg ctgcaggcga gcaaaggaac agcgccgcgt
    44941 tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt gtgttgtaac attttttgtt
    45001 caatttctgg tgtaataaca gatttcaggg catcggttaa tattgccccc ggcgcaatgc
    45061 cattcacccg aatatttttt tcacccaggt caaacgccat atttctgacc agatgactgg
    45121 ccgcagcttt agatgatgca taggaggtca tgtttatatt tttattttct gccgccatag
    45181 aagtgatagt cagaataacg ccaccgccat ttttttccat ttctggcgca acaagttgtg
    45241 acagatggaa aaaagaaaac acattcagtt cataagcgcg acgaaaatct gccattggca
    45301 tatcaaacgg tttaggccca ccgccaccgg cgttgttaac caggatatca actttaccca
    45361 gcttactgac ggcaaagtct gccagtgcag agagttcctg ttcggaagta atatcgcagc
    45421 ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc tacaacatga ttagctgcat
    45481 cggcattaat atcactgacc accacagatg cgccagctgt cgcgaatgta atggcaattt
    45541 ctttaccaat acctgcaccc gcacctgtga tgatggcgca ttttccgtcg agtctcaggt
    45601 tgtcagaatt aaacacataa acctcctgtg aacttcagta aaaataaaag gcaccgcctg
    45661 tgtgccacat tttttatcat taaccttgag gaaaggacac taccaggtat agcagataaa
    45721 gagaggatta tttcgccgca atgagccggg cgggaataat aagattgcgt gaatgcgtct
    45781 cttcatgggt gattttttgc atcatgcgat ccgcaagtgt ggtaccaagt tcccgcgcag
    45841 gcgtgctggc ccaagtaaca ggtatatcat caagtgtggt tggcgttgca tcggtaaatg
    45901 ccgccaacga aacctgttgt tcaaagtaac gatcgactcc gctttccccg ctttgccgcc
    45961 ctgctttcag caaaccaaac catgccccca tcgcaatagt ttcgttatag caaaccacag
    46021 cactgatggt cggattatga cgtaaaagcg ccgtgatagc ttccgcggct tgcttctggc
    46081 tggaagagca ctccaacacc caatcgctgt gaaacggcag gccaaatttt agtagagttg
    46141 cacaatagcc ccccacccgc tctgcccggg ttaatgagga actttgccct cccagccagg
    46201 cgatacgctg atgcccattg cgaatgagat gctccgtcaa caactgtgca gcctgcatgt
    46261 tgtccgggcg aaccgtatca acatcatcaa gataactggc acgggaagcg aaaatcaccg
    46321 ggatagcttt ttcttctgcc atccgtcgca ggtcatcgct gcttcctgca gccccggcaa
    46381 tgaccacacc atcgaccccc tgattcagta acagtgaaaa ccgctgtgcc agctgctcgc
    46441 cgtctttacc gccgtgaagc aaaaaaacca tccttccctg cgcttccaga gcttccgtca
    46501 atccggccgt taattcggcg taaaacggcg cagataaatc acggacgatc aaaccaatga
    46561 cgccgctttc cccgccgcgc agcgccgacg cctgacgatt gcgcacaaat cccagctctt
    46621 caatggcggc gttgacgcgt tctcctgtgg cggtagagat tcgccctttg ccacttagca
    46681 ccagcgaaac ggtgcttacc gacacgcccg cagccagcgc aacatcatga atggttattt
    46741 ttttggcggt agccatgcag atgacctact ccctgattat gtgacagata aaacgtttta
    46801 ccttttattt attttatacc cgctattatc gttgcgtaat gtgatttatg cctcactaaa
    46861 atttgataaa acgttttatc ttctcgcgca atttactgaa tccagattgt tctctacgag
    46921 gagtcgtttt atgacggcga aaacagcacc gaaagtcacg ctgtgggagt tcttccagca
    46981 gttaggcaaa accttcatgt tacccgtggc attattgtcg ttctgcggca ttatgctcgg
    47041 cattggtagt tctcttagca gccatgatgt catcaccctg atcccggtcc tgggcaaccc
    47101 cgtgttgcag gctatcttta cctggatgag taagattggc tcgtttgctt ttagtttcct
    47161 gccagtcatg ttctgtatcg ccatcccgct gggcctggca cgcgaaaaca aaggcgtagc
    47221 ggcattcgct ggcttcgtcg gttatgcggt aatgaacctc gcggtaaact tctggttgac
    47281 caataaaggc attctgccaa ccacggacgc cgcggttctg aaagccaata acattcagag
    47341 cattcttggg atccagtcga tcgacaccgg gatcctcggt gcggtgatcg ccggtattat
    47401 cgtctggatg ctgcatgagc gtttccataa tatccgcctg ccagatgcgc tggcattctt
    47461 cggcggtacg cgcttcgtac caatcatctc ctcgctggtg atgggtcttg tcggcctggt
    47521 gattccatta gtctggccaa ttttcgccat gggtattagc ggattaggcc atatgatcaa
    47581 cagcgcgggt gatttcggac cgatgctgtt tggtaccggt gaacgtctgc tgttgccgtt
    47641 tggtctacat cacattctgg tggcattaat tcgctttacc gacgcaggcg gcacgcagga
    47701 agtctgcggt caaaccgtca gcggtgcact gaccatcttc caggcgcaat tgagttgccc
    47761 gaccactcac ggtttttctg aaagcgccac gcgtttcctt tcgcaaggta aaatgcctgc
    47821 gtttctcggc ggtctgccag gtgcagcgtt agctatgtat cactgtgcgc gcccggaaaa
    47881 tcgccataaa attaaaggtc tgctgatttc tggcctgatc gcctgtgtcg ttggcggcac
    47941 gaccgaaccg ctggaattcc tgttcctgtt cgtagcgcca gttctgtatg tcatccacgc
    48001 gctgttaacc ggcctcggct tcactgtcat gtctgtgctc ggcgtcacca tcggtaatac
    48061 cgacggcaat atcatcgact ttgtggtgtt cggtattttg catggtctgt caaccaagtg
    48121 gtacatggtg ccagtagtgg cggcaatatg gtttgtcgtt tactacgtca tcttccgttt
    48181 cgctatcacc cgcttcaatc tgaaaacccc ggggcgcgat agtgaagttg ccagttcaat
    48241 cgaaaaagcc gttgccggtg cgccgggtaa atcaggttac aacgttccgg caatcctcga
    48301 agccttaggc ggagccgaca atattgtcag tctcgataac tgcattaccc gtctgcgttt
    48361 gtctgtaaaa gatatgtcgc ttgttaatgt gcaggcactg aaggacaatc gggcaattgg
    48421 cgtagtacaa cttaatcaac ataacctgca ggttgttatc gggccacaag ttcagtcagt
    48481 aaaagatgaa atggccggtc tgatgcatac tgtccaggca taaggataag atatgttcga
    48541 tttttcaaag gtcgtggatc gtcatggcac atggtgtacg cagtgggatt atgtcgccga
    48601 ccgtttcggc actgctgacc tgttaccgtt cacgatttca gacatggatt ttgccactgc
    48661 cccctgcatt atcgaggcgc tgaatcagcg tctgatgcac ggcgtgtttg gctacagccg
    48721 ctggaaaaac gatgagtttc tcgcggctat tgcccactgg ttttccaccc agcattacac
    48781 cgccatcgat cctcagtcgg tggtgtatgg cccttctgtc atctatatgg tttcagaact
    48841 gattcgtcag tggtctgaaa caggtgaagg cgtggtgatc cacacacccg cctatgacgc
    48901 attttacaag gccattgaag gtaaccagcg cacagtaatg cccgttgctt tagagaagca
    48961 gcctgacggc tggttttgcg atatgggcaa gctggaagcc gtgttggcga aaccagaatg
    49021 taaaattatg ctcctgtgta gcccacagaa tcctaccggg aaagtgtgga cgtgcgatga
    49081 gctggagatc atggctgacc tgtgcgagcg tcatggtgtg cgggttattt ccgatgaaat
    49141 ccatatggat atggtttggg gcgagcagcc gcatattccc tggagtaatg tggcgcgcgg
    49201 agactgggcg ttgctaacgt cgggatcaaa aagtttcaat attcccgccc tgaccggtgc
    49261 ttacgggatt atagaaaata gcagtagccg cgatgcctat ttatcggcac tgaaaggacg
    49321 tgatgggctt tcttcccctt cagtactggc gttaactgct catatcgccg cctatcagca
    49381 aggcgcaccg tggctggatg ccttacgcgt ctatctgaaa gataacctga cgtatatcgc
    49441 agataaaatg aacgctgcgt ttcctgaact caactggcag atcccgcaat ccacttatct
    49501 ggcctggctt gatctacgtc cattgaatat tgacgacaac gcgttgcaaa aagcgcttat
    49561 cgagcaagaa aaggtcgcga tcatgccggg atatacctac ggtgaagaag gtcgtggttt
    49621 tgtccgactc aatgccggct gcccacgttc gaaactggaa aaaggtgtgg ctggattaat
    49681 taacgccatc cgcgctgttg gttaacccca attgcgcaac gtaaaaaatc gttgcgcaat
    49741 cgtggatttt taccctgctt tgtttttata atggtgcgca cttttatatc cagaaaaaga
    49801 gtgcgaccat gattgatacc accctgccat taactgatat ccatcgccac cttgatggca
    49861 acattcgtcc ccagaccatt cttgaacttg gccgccaata taatatctcg cttcctgcac
    49921 aatccctgga aacactgatt ccccacgttc aggtcattgc caacgaaccc gatctggtga
    49981 gctttctgac taaacttgac tggggcgtta aagttctcgc ctctcttgat gcctgccgcc
    50041 gcgtggcatt tgaaaacatt gaagatgcag cccgtaacga cctgcactat gtcgagctgc
    50101 gtttttcacc aggctacatg gcaatggcac atcagctgcc tgtatcgggt gttgtcgaag
    50161 cggtgatcga tggcgtacgt gaaggttgcc gcacctttgg tgtgcaggcg aagcttatcg
    50221 gtattatgag ccggaccttc ggcgaagccg cctgtcagca agagctggag gcctttttag
    50281 cccaccgtga ccagattacc gcacttgatt tagccggtga tgaacttggt ttcccgggaa
    50341 gtctgttcct ttctcatttc aaccgcgcgc gtgatgcggg ctggcatatt accgtccatg
    50401 caggcgaagc tgccggaccg gaaagcatct ggcaggcgat tcgtgaactg ggggcggagc
    50461 gtattggaca tggcgtaaaa gccattgaag atcgggcgct gatggatttt ctcgccgagc
    50521 aacaaattgg tattgaatcc tgtctgacct ccaatattca gaccagcacc gtggcggatc
    50581 tggctgcaca tccgctgaaa acgttccttg agcatggcat tcgtgccagc attaacactg
    50641 acgatcccgg cgtgcaggga gtggatatca ttcacgaata taccgttgcc gcgccagctg
    50701 ctgggttatc ccgcgagcaa atccgccagg cacagattaa tggtctggaa atggctttcc
    50761 tcagcgcaga ggaaaaacgc gcactgcgag aaaaagtcgc cgcgaagtaa caaaaatgga
    50821 tggtgctaat tgcaccatcc ccttttcacg caaggcacaa agtcgcgcga tgtttggcgg
    50881 attcgatgcc cagctcaatc aactccatta cctggattgc ctgacttgcc ggaaccggat
    50941 tttcaccatc gccatttaac gcatcacgaa tagccgcata gtaagccgga taattccccg
    51001 gcactgtcaa cagcgtttct tcgacacgtt cctcaccttc cacgcgggta agtacgccat
    51061 cgcgcatatc gtagccccag tcttcctgcg gcagacgctc gccatttttc agacattctt
    51121 cctgtggatc gaggccatat ttcacataac tgcctcggga tccatgcacg atataccgtg
    51181 ctgactcggc tgctgccagc atggtgccgt gtaaaatcac tcgccgctgc ggataggaca
    51241 agatagcgtg gaaataatcg gtcgactgcg ctccgggccg taactgtgcc aaatctaccg
    51301 tcatgctaac cggtaaacca aatagcgtaa tggcctgatc aagaaggtgc ggtgctaaat
    51361 cgtaccagat accgctgcct gggccgccct gttcacgcca acgatcgcgc acctgcggac
    51421 ggaagcggtc aaaatgagac tcaaagtaag caacttcacc cagcacgcct tccgcgagca
    51481 aaccttttag cgtcaggaaa tcgctatccc agcgacggtt atggaataca gacagcactc
    51541 gccccaggct ttttgccagc gcatccagct ctcgcgcttg tgacagtgtc acggtaaagg
    51601 gtttatcaac gaccacatgt ttacccgcct caagcgccgc tttggctaac ggaaaatggg
    51661 tatcgttggg tgtaggaatg acaatcaggt ctatgttggg atcgttaaac agatgcttcg
    51721 gctcagagac aaccgtaacc gttggccagt cagcttttac ttttgtttca tcgctgctgg
    51781 agattaccgc cagctccagc ccgggcgtgc ccgcaatcag gggcgcatgg aaggttttgc
    51841 tcgcataacc atacccaatc aacccaacac ggatgttgtc gctcatgtca ttgcctctca
    51901 ttgaagtatg atggctattt gacactatcc tttatcctcg ctcaacagtt taataacctg
    51961 ccagcaataa gggatgttgt ttaacttaag tcaaaaaaat agcgattttt ccaacgacaa
    52021 aagctaaata tcgcaaaaac ctcagtaaaa atcttgctgg agctattatt gctaagtaac
    52081 atttaccccc tgaagttaat ggatcaatca acagagatgt gggctgtaat gaatcgtctt
    52141 attgaattaa caggttggat cgttcttgtc gtttcagtca ttcttcttgg cgtggcgagt
    52201 cacattgaca actatcagcc acctgaacag agtactacag tacaacacaa gtaagctctg
    52261 cacttgttga gcgacatgct gcccgtccgg gtgcatgttt tcacttgtcg gatattaaac
    52321 caggaattta ttatcttgtt cgatgttgtt gatgattgtc agggatagta aagcgcgtta
    52381 ctctttccag agcagttttt catccctgaa tatttgccat cagcacattc ttattattgt
    52441 aataacacaa gcgttcactt ttggtgaaat tttatcatca ctaataatac ctaccccttt
    52501 ggtattaatt attttatgga ccttttatga ctgttcagga ctacttatta aaatttcgca
    52561 aaatcagttc actcgaaagt ctggaaaaac tctacgacca tcttaattac accctgacgg
    52621 acgatcagga actgatcaat atgtatcgtg ctgccgatca ccgtcgcgca gagctggttt
    52681 ctggcgggcg tttgtttgac ctcggccagg taccgaagtc cgtctggcac tatgtccagt
    52741 aaagaaagta gcgatcggtt ataaaagcct tataataacg cccctaaaat tagatttgtc
    52801 tgtttgcgtc gctattctgg agaacctatg accaccacaa cgccacaacg tattggaggc
    52861 tggttgcttg gcccgttggc ctggctttta gtcgctctgt tgagtacgac gctggcgttg
    52921 ttgctgtata ccgctgcgtt atcttctccg caaacatttc aaacgctcgg cggacaagca
    52981 ctgacgacac aaattttatg gggcgtttct tttattaccg ctatcgccat gtggtattac
    53041 acactgtggt tgaccatcgc tttctttaaa cgtcggcgtt gtgttcctaa acactatatt
    53101 atctggctgc ttatttccgt attgctggca gtaaaagcgt tcgctttttc acccgtagaa
    53161 gatggcatag ccgttcgcca gttgctgttt actttgttgg caaccgcatt gatcgtcccc
    53221 tatttcaaac gctcgtcgcg ggtgaaagcg acgtttgtga atccgtaata accttacagt
    53281 taacctgttg tcgcctgctc tggattaacg gataataggc ggctttttta tttcaggccg
    53341 aaaaatgact gactacctgt tactgtttgt cggaactgta ctggtcaata actttgtact
    53401 ggtcaagttt ctcggtctct gtccgtttat gggggtttcc aaaaagctgg aaaccgcgat
    53461 gggcatgggg ctggcaacaa cgtttgtaat gacgctggcg tctatttgcg cctggcttat
    53521 cgatacgtgg attttgatcc cacttaatct gatttacctg cgcaccctgg cgtttattct
    53581 ggtgattgct gtggttgtgc agttcaccga aatggtggtg cgcaaaacca gcccggtact
    53641 ttaccgtctg ctggggattt ttttgccgct tatcaccacc aactgtgccg tgctcggcgt
    53701 ggcgttgctg aatatcaatc tcgggcacaa tttcttgcag tcagcgctgt acggtttttc
    53761 cgccgctgtc ggtttctcgc tggtgatggt gctcttcgcc gccatccgcg aacgccttgc
    53821 tgtggctgat gtcccggcac cttttcgcgg taatgccatt gcgttaatta ccgcaggtct
    53881 tatgtctctg gcctttatgg gctttagtgg tttggtgaag ttgtaatgaa tgctatctgg
    53941 attgccgttg ccgccgtgag cctgctgggc ctggcgtttg gcgccattct gggttatgcc
    54001 tcccgccgtt ttgcggtgga agacgatccg gtcgttgaga aaattgacga aatcttaccg
    54061 cagagccagt gtggtcagtg cggttatccc ggctgtcgcc cctacgcgga agccatcagc
    54121 tgtaacggtg aaaaaatcaa ccgttgcgcc ccaggtggcg aagctgtgat gctaaaaatt
    54181 gccgagttgc ttaatgtcga gccgcagccg ctggatggcg aagcgccaga gctaacgcca
    54241 gcgcggatgg tggcggttat tgatgaaaat aactgtattg gctgcactaa atgtattcag
    54301 gcgtgtccgg tagacgccat cgttggcgct acccgcgcca tgcatacggt aatgagtgat
    54361 ctctgtacgg gctgcaattt atgtgttgat ccgtgcccga cgcactgcat ctcgttgcaa
    54421 ccggtcgcag aaactcctga ctcctggaaa tgggatctga acaccattcc cgtgcgtatc
    54481 attcccgtgg aacaccatgc ttaagttatt ctctgcattc agaaaaaata aaatctggga
    54541 tttcaacggc ggcatccatc caccggagat gaaaacccag tccaacggta cgcccctgcg
    54601 ccaggtacca ctggcgcagc gttttgtcat tccactgaaa caacatattg gcgctgaagg
    54661 tgagttgtgc gttagcgtcg gcgataaagt attgcgcggc cagccgctta cccgtggtcg
    54721 cggcaaaatg ctgccggttc acgcgcccac ctcgggtacc gttacggcta ttgcgcccca
    54781 ctctacggct catccttcag ctttagctga attaagtgtg attattgatg ccgatggcga
    54841 agactgctgg atcccgcgcg acggctgggc cgattatcgc tctcgcagtc gcgaagagtt
    54901 aatcgagcgc attcatcagt ttggtgttgc cgggctgggt ggtgcgggat ttccgacagg
    54961 cgttaaattg cagggcggcg gagataagat tgaaacgttg ataatcaacg ctgctgagtg
    55021 cgagccgtac attaccgccg atgaccgttt gatgcaggat tgcgcggctc aggtcgtaga
    55081 gggtatccgc atccttgcgc atattctgca gccacgcgaa attcttatcg gtattgaaga
    55141 taacaaaccg caggcgattt ccatgctgcg cgcggtgctg gcagactctc acgatatttc
    55201 tctgcgggtg attccaacca aatatccttc tggcggtgct aaacaattaa cctacattct
    55261 gaccgggaag caggttccac atggcgggcg ttcatccgat atcggcgtat taatgcaaaa
    55321 cgtcggcacc gcttatgcag tgaaacgtgc cgttattgat ggcgagccga ttaccgagcg
    55381 tgtcgtgacc ctgactggcg aagcaatcgc tcgcccaggc aacgtctggg cacggttggg
    55441 gacgccagtg cgtcatttat tgaatgatgc cggattctgc ccctctgccg atcaaatggt
    55501 gattatgggc ggcccgctaa tgggctttac cttgccgtgg ctggatgtcc cggtcgtaaa
    55561 gattaccaac tgtcttttgg ctccctctgc caatgaactt ggcgaaccac aggaagaaca
    55621 aagttgcatc cgttgtagcg cctgtgctga cgcctgccct gcggatcttt tgccgcaaca
    55681 gttgtactgg ttcagcaaag gtcagcaaca cgataaagcc accacgcata acattgccga
    55741 ctgcattgaa tgtggagctt gcgcgtgggt ttgcccgagc aatatccccc tggtgcaata
    55801 tttccgtcag gaaaaagctg aaattgcggc tattcgtcag gaagaaaagc gcgccgcaga
    55861 agccaaagcg cgtttcgaag cgcgccaagc tcgtctggag cgcgaaaaag cggctcgcct
    55921 tgaacgacat aagagcgcag ccgttcaacc tgcagccaaa gataaagatg cgattgctgc
    55981 cgctctggcg cgggtgaaag agaaacaggc ccaggctaca cagcctattg tgattaaagc
    56041 gggcgaacgc ccggataaca gtgcaattat tgcagcacgg gaagcccgta aagcgcaagc
    56101 cagagcgaaa caggcagaac tgcagcaaac taacgacgat gcaaccgttg ctgatccacg
    56161 taaaactgca gttgaagcgg ctattgcccg cgccaaagca cgtaagctgg aacagcaaca
    56221 ggctaatgcg gagccagaag aacagattga tccgcgcaaa gccgccgtcg aagctgctat
    56281 tgcccgtgcc aaagcacgca agctggaaca gcaacaggct aatgcggagc cagaagaaca
    56341 gattgatccg cgcaaagctg ccgtcgaagc ggctatcgcc cgcgccaaag cacgcaagct
    56401 ggaacagcaa caggccaatg cggagccaga acaacaggtc gatccgcgca aagctgccgt
    56461 cgaagcggct attgcacgcg ccaaagcgcg caagctggaa cagcaacaac aggctaatgc
    56521 ggaaccagaa gaacaggttg atccacgcaa agccgccgtc gaagcggcta ttgcccgtgc
    56581 caaagcacgc aagctggaac agcaacaggc taatgcagag ccagaagaac agattaatcc
    56641 gcgcaaagcg gcagttgctg cggctattgc ccgcgttcag gccaaaaaag ccgctcagca
    56701 aaaggttgta aatgaggact aaatggtatt cagaatagct agctcccctt atacccataa
    56761 ccagcgccag acatcgcgca ttatgctgtt ggtattgctc gcagccgtgc caggaatcgc
    56821 agcgcaactg tggttttttg gttggggtac tctcgttcag atcctgttgg cgtcggtcag
    56881 tgctcttctg gccgaagctc tcgtcctcaa actacgcaag cagtcggtag ctgcaacgtt
    56941 gaaagataac tcagcattgc tgacaggctt attgctggcg gtaagtattc cccccctcgc
    57001 gccatggtgg atggtcgtgc tgggtacggt gtttgcggtg atcatcgcta aacagttgta
    57061 tggcggtctg gggcaaaacc cgtttaatcc ggcaatgatt ggttatgtgg tcttactgat
    57121 ctccttcccg gtgcagatga ccagttggtt accgccacat gaaattgcgg tcaacatccc
    57181 tggttttatc gacaccatac aggttatttt cagtggtcat accgccagtg gtggtgatat
    57241 gaacacgcta cgcttaggta ttgatggcat tagtcaggcg acaccgctgg atacatttaa
    57301 aacctctgtc cgtgccggtc attcggttga agagattatg caatatccga tctacagcgg
    57361 tattctggcg ggcgctggtt ggcaatgggt aaatctcgcc tggctggctg gcggcgtgtg
    57421 gttgctatgg cagaaagcga ttcgctggca tattcccctc agcttcttag caacgctggc
    57481 gttatgcgca acgttgggct ggttgttctc accagatact ctggcagcac cgcaaattca
    57541 tctgctgtct ggtgcgacca tgctcggcgc attctttatt ttgactgacc cggttaccgc
    57601 ttctacgacc aatcgtggtc gtcttatttt cggcgcgctg gcgggcttat tagtctggtt
    57661 gatccgcagt ttcggcggct atcctgacgg cgtggctttt gccgtcctgc tggcgaacat
    57721 cacggttcct ctgatcgatt actacacgcg tccgcgcgtc tacggccatc gcaaagggta
    57781 aaccatgctg aaaactatcc gaaaacacgg cattacgttg gcgctctttg cagcgggttc
    57841 aacagggtta actgcggcca tcaaccagat gaccaaaacg acaattgctg aacaggccag
    57901 tctgcaacaa aaggcgttat ttgatcaggt gctgccagct gaacgctata acaatgcgct
    57961 ggcacagagt tgctatctgg taactgcgcc agagttaggt aaaggtgagc atcgggttta
    58021 catcgccaaa caggatgaca aaccggtagc tgccgttctg gaagcaaccg cgccagatgg
    58081 ctattccggt gcgattcagc tgctggtggg agccgatttt aacggcacgg tacttggcac
    58141 gcgcgtgact gagcaccacg aaacgccagg gcttggcgat aaaatcgaac tgcgcctttc
    58201 tgactggatc acccattttg cgggtaaaaa aatcagtggt gcggatgatg cgaactgggc
    58261 ggtgaagaaa gatggtggtg atttcgacca gttcaccggc gcgacgatta ctccccgcgc
    58321 ggtggttaat gcggtaaaac gcgccggatt gtacgctcag acgttaccgg cacaactttc
    58381 tcaacttcct gcctgtggag aataaaacgt gagcgaaatt aaagacgtta ttgttcaggg
    58441 gttgtggaaa aacaactctg cgctggtcca gttgctcggc ctttgtccgc tgttggcggt
    58501 cacgtccact gccactaacg ctctgggttt aggacttgcg actacgctgg tactgacgct
    58561 aaccaacctg accatttcga cgctgcgtca ctggacgccc gccgagatcc gcattcccat
    58621 ttacgtgatg atcatcgcct cggtggtcag cgctgtacag atgctgatca acgcctacgc
    58681 ctttggcctg tatcaatcat tagggatttt tattccgctg attgtcacta actgtatcgt
    58741 tgtgggccgc gctgaagcct tcgccgccaa aaaaggtccg gcgctttcgg cactggacgg
    58801 cttttcaatt ggtatgggcg caacctgcgc catgttcgtg ctgggttcac tacgcgaaat
    58861 tatcggcaat ggcacgttgt ttgacggtgc agatgcgctg ttaggtagct gggcaaaagt
    58921 attacgcgtg gagattttcc acaccgactc ccctttcctg ctggcgatgc tgccaccagg
    58981 tgcatttatt ggcctgggac tgatgctggc aggaaaatac ctgattgatg aaaaaatgaa
    59041 aaagcgccgt actgaagcag ctgccgaacg tgcattgcca aacggtgaaa cagggaatgt
    59101 ctgatgaaca aagcaaaacg cctggagatc ctcactcgcc tgcgtgagaa caatcctcat
    59161 cccaccaccg agcttaattt cagttcgcct tttgaattgc tgattgccgt actgctttcc
    59221 gctcaggcga ccgatgtcag tgttaataag gcgacggcga aactctaccc ggtggctaat
    59281 acgcctgcag cgatgcttga actgggcgtt gaaggggtga aaacctatat caaaacgatt
    59341 ggcctttata acagcaaagc agaaaatatc atcaaaacct gccgtatatt actggaacaa
    59401 cataatggcg aggttccgga agatcgtgct gcgcttgaag ccctgcctgg tgtaggtcgt
    59461 aaaacagcca acgtcgtatt aaacactgca ttcggctggc cgactattgc tgtcgacacg
    59521 cacattttcc gcgtttgtaa tcgtacacaa tttgcgccgg ggaaaaacgt cgaacaggta
    59581 gaagaaaagc tactgaaagt ggttccggca gagtttaaag tcgactgcca ccattggttg
    59641 atcctgcacg ggcgttatac ctgcattgcc cgcaagcctc gctgtggctc ttgtattatt
    59701 gaagatcttt gtgaatacaa agagaaagtt gacatctaaa gatcaggggt aacaccgatt
    59761 accctattca taacctttct ttatcctctt ttaaaacatc tttttaacgt caatgatgcc
    59821 attgcttagc gttatcatca ggtaatccgt ttgcagataa ccaaaaatgc aggttaattg
    59881 tttttttaat agcgaaattt tctattcatt cgtgatcaag atcacgatgc tgtggcaatg
    59941 taaaaacatt gcaataatca ggttaaaatc atttaaattt acacacgcaa caaatattga
    60001 cctacaaaac attacactgg ctatttttca gaaactggac tatctcacta ggaaaaacgc
    60061 caaatagt
//
LOCUS       DADSZD010000008        58632 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.008, whole genome shotgun sequence.
ACCESSION   DADSZD010000008 DADSZD010000000
VERSION     DADSZD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.008"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..192)
                     /locus_tag="J1Z28_000417"
     CDS             complement(<1..192)
                     /locus_tag="J1Z28_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715283.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKK"
     gene            complement(255..1727)
                     /gene="der"
                     /locus_tag="J1Z28_000418"
     CDS             complement(255..1727)
                     /gene="der"
                     /locus_tag="J1Z28_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBA7715284.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1845..3023)
                     /gene="bamB"
                     /locus_tag="J1Z28_000419"
     CDS             complement(1845..3023)
                     /gene="bamB"
                     /locus_tag="J1Z28_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBA7715285.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTVWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3034..3654)
                     /locus_tag="J1Z28_000420"
     CDS             complement(3034..3654)
                     /locus_tag="J1Z28_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HBA7715286.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3672..4946)
                     /gene="hisS"
                     /locus_tag="J1Z28_000421"
     CDS             complement(3672..4946)
                     /gene="hisS"
                     /locus_tag="J1Z28_000421"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBA7715287.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQVL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5057..6175)
                     /gene="ispG"
                     /locus_tag="J1Z28_000422"
                     /gene_synonym="gcpE"
     CDS             complement(5057..6175)
                     /gene="ispG"
                     /locus_tag="J1Z28_000422"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBA7715288.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6202..7209)
                     /gene="rodZ"
                     /locus_tag="J1Z28_000423"
     CDS             complement(6202..7209)
                     /gene="rodZ"
                     /locus_tag="J1Z28_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBA7715289.1"
                     /translation="MNTEATHDQNETLTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7494..8648)
                     /gene="trmG/rlmN"
                     /locus_tag="J1Z28_000424"
     CDS             complement(7494..8648)
                     /gene="trmG/rlmN"
                     /locus_tag="J1Z28_000424"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBA7715290.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8798..9229)
                     /gene="ndk"
                     /locus_tag="J1Z28_000425"
     CDS             complement(8798..9229)
                     /gene="ndk"
                     /locus_tag="J1Z28_000425"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBA7715291.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAARESAYFFGEGEVCPRTR"
     gene            complement(9378..11690)
                     /gene="pbpC"
                     /locus_tag="J1Z28_000426"
     CDS             complement(9378..11690)
                     /gene="pbpC"
                     /locus_tag="J1Z28_000426"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBA7715292.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSHERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAIPLLNQVNNILLS
                     RSVNFPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11691..16652)
                     /gene="yfhM"
                     /locus_tag="J1Z28_000427"
     CDS             complement(11691..16652)
                     /gene="yfhM"
                     /locus_tag="J1Z28_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBA7715293.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTTVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSHNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKNGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPVKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMISIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDAMRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16859..17704
                     /gene="sseA"
                     /locus_tag="J1Z28_000428"
     CDS             16859..17704
                     /gene="sseA"
                     /locus_tag="J1Z28_000428"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBA7715294.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPEK"
     gene            complement(18201..18977)
                     /gene="sseB"
                     /locus_tag="J1Z28_000429"
     CDS             complement(18201..18977)
                     /gene="sseB"
                     /locus_tag="J1Z28_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBA7715295.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPVQMIDSLTT
                     LFKTIKPVKRAFICLIKESEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19119..20402)
                     /gene="pepB"
                     /locus_tag="J1Z28_000430"
     CDS             complement(19119..20402)
                     /gene="pepB"
                     /locus_tag="J1Z28_000430"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBA7715296.1"
                     /translation="MTEAMKITLSPQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRNQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20461..20661)
                     /gene="iscX"
                     /locus_tag="J1Z28_000431"
     CDS             complement(20461..20661)
                     /gene="iscX"
                     /locus_tag="J1Z28_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBA7715297.1"
                     /translation="MGLKWTDSREIGEALYDAYPDIDPKTVRFTDMHQWICALEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20673..21008)
                     /gene="fdx"
                     /locus_tag="J1Z28_000432"
     CDS             complement(20673..21008)
                     /gene="fdx"
                     /locus_tag="J1Z28_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBA7715298.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21010..22860)
                     /gene="hscA"
                     /locus_tag="J1Z28_000433"
     CDS             complement(21010..22860)
                     /gene="hscA"
                     /locus_tag="J1Z28_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBA7715299.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDAVSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22877..23392)
                     /gene="hscB"
                     /locus_tag="J1Z28_000434"
     CDS             complement(22877..23392)
                     /gene="hscB"
                     /locus_tag="J1Z28_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBA7715300.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23488..23811)
                     /gene="iscA"
                     /locus_tag="J1Z28_000435"
     CDS             complement(23488..23811)
                     /gene="iscA"
                     /locus_tag="J1Z28_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBA7715301.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23828..24214)
                     /gene="iscU"
                     /locus_tag="J1Z28_000436"
     CDS             complement(23828..24214)
                     /gene="iscU"
                     /locus_tag="J1Z28_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBA7715302.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDETVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24242..25456)
                     /gene="iscS"
                     /locus_tag="J1Z28_000437"
     CDS             complement(24242..25456)
                     /gene="iscS"
                     /locus_tag="J1Z28_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBA7715303.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25568..26056)
                     /gene="iscR"
                     /locus_tag="J1Z28_000438"
     CDS             complement(25568..26056)
                     /gene="iscR"
                     /locus_tag="J1Z28_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBA7715304.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26327..27067)
                     /gene="trmJ"
                     /locus_tag="J1Z28_000439"
     CDS             complement(26327..27067)
                     /gene="trmJ"
                     /locus_tag="J1Z28_000439"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBA7715305.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27186..27989
                     /gene="suhB"
                     /locus_tag="J1Z28_000440"
     CDS             27186..27989
                     /gene="suhB"
                     /locus_tag="J1Z28_000440"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBA7715306.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28134..28988
                     /locus_tag="J1Z28_000441"
     CDS             28134..28988
                     /locus_tag="J1Z28_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7715307.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLTHGKADHVIPWQHSEKLYSLTKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29179..30459
                     /gene="csiE"
                     /locus_tag="J1Z28_000442"
     CDS             29179..30459
                     /gene="csiE"
                     /locus_tag="J1Z28_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBA7715308.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTANTATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSRFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGATLIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(30451..31590)
                     /locus_tag="J1Z28_000443"
     CDS             complement(30451..31590)
                     /locus_tag="J1Z28_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBA7715309.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31959..32381
                     /locus_tag="J1Z28_000444"
     CDS             31959..32381
                     /locus_tag="J1Z28_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBA7715310.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIILGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNISIAGAFLLLAITGPGAISLDRR"
     gene            complement(32541..33413)
                     /locus_tag="J1Z28_000445"
     CDS             complement(32541..33413)
                     /locus_tag="J1Z28_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBA7715311.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSRSDDSLC
                     LVYEHLSGVYHYRVSQAFHLTADTLTVTLAVTNQGAETLPFGTGWHPYFPLSPQTRVQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLTPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33425..34519)
                     /locus_tag="J1Z28_000446"
     CDS             complement(33425..34519)
                     /locus_tag="J1Z28_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBA7715312.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVVVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34552..35550)
                     /locus_tag="J1Z28_000447"
     CDS             complement(34552..35550)
                     /locus_tag="J1Z28_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715313.1"
                     /translation="MSASSLPLPHGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35575..37086)
                     /locus_tag="J1Z28_000448"
     CDS             complement(35575..37086)
                     /locus_tag="J1Z28_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7715314.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37109..38092)
                     /locus_tag="J1Z28_000449"
     CDS             complement(37109..38092)
                     /locus_tag="J1Z28_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBA7715315.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38189..41470)
                     /locus_tag="J1Z28_000450"
     CDS             complement(38189..41470)
                     /locus_tag="J1Z28_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBA7715316.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHEHQSQALPLPDVAKA
                     PLAAQDITSTDETWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVVYASQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDTGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPAL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDDREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAKEMAKTSIEADFFAVSQPDLL
                     SLYSDLQQQHKEKCLMVAMLAAAGLGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41588..42781
                     /locus_tag="J1Z28_000451"
     CDS             41588..42781
                     /locus_tag="J1Z28_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA7715317.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQVRDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDHSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42845..44098)
                     /locus_tag="J1Z28_000452"
     CDS             complement(42845..44098)
                     /locus_tag="J1Z28_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBA7715318.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44427..45617
                     /gene="hmpA"
                     /locus_tag="J1Z28_000453"
     CDS             44427..45617
                     /gene="hmpA"
                     /locus_tag="J1Z28_000453"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBA7715319.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGLSLPRFTAHTWYRQPNEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45662..46000)
                     /gene="glnB"
                     /locus_tag="J1Z28_000454"
     CDS             complement(45662..46000)
                     /gene="glnB"
                     /locus_tag="J1Z28_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBA7715320.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46061..47395)
                     /gene="glrR"
                     /locus_tag="J1Z28_000455"
     CDS             complement(46061..47395)
                     /gene="glrR"
                     /locus_tag="J1Z28_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBA7715321.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMTRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47385..48098)
                     /gene="qseG"
                     /locus_tag="J1Z28_000456"
     CDS             complement(47385..48098)
                     /gene="qseG"
                     /locus_tag="J1Z28_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBA7715322.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNRPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     LHESEKPAPSTDEVTPDEP"
     gene            complement(48263..49690)
                     /gene="qseE"
                     /locus_tag="J1Z28_000457"
     CDS             complement(48263..49690)
                     /gene="qseE"
                     /locus_tag="J1Z28_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBA7715323.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50266..54153)
                     /gene="purL"
                     /locus_tag="J1Z28_000458"
                     /gene_synonym="purI"
     CDS             complement(50266..54153)
                     /gene="purL"
                     /locus_tag="J1Z28_000458"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBA7715324.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEHLFAHHQPTPVTS
                     IDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFEITPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTREVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHVL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DGAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            54411..55967
                     /gene="mltF"
                     /locus_tag="J1Z28_000459"
     CDS             54411..55967
                     /gene="mltF"
                     /locus_tag="J1Z28_000459"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBA7715325.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTTKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55964..56500)
                     /gene="tadA"
                     /locus_tag="J1Z28_000460"
     CDS             complement(55964..56500)
                     /gene="tadA"
                     /locus_tag="J1Z28_000460"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBA7715326.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(56525..57160)
                     /gene="yfhb"
                     /locus_tag="J1Z28_000461"
     CDS             complement(56525..57160)
                     /gene="yfhb"
                     /locus_tag="J1Z28_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBA7715327.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57369..58217
                     /locus_tag="J1Z28_000462"
     CDS             57369..58217
                     /locus_tag="J1Z28_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7715328.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVAMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58273..58533
                     /gene="yfhL"
                     /locus_tag="J1Z28_000463"
     CDS             58273..58533
                     /gene="yfhL"
                     /locus_tag="J1Z28_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBA7715329.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        13894 a        16342 c        14593 g        13803 t
ORIGIN      
        1 ttttttagat attaaaccgt gcccatgttg gcagaaataa tccacagcac cacaagcttt
       61 taacacttgt aacgcctgat gacactcagg gcaaagtgcc tggacggtaa agtctttgtc
      121 gcaagttgca cagtgagcag tctcgccatt gcgttcgaga ggggttttgc aggttggaca
      181 gttgagtgcc ataaatactc ctcgctcgtt attcggtttc cctttccgtg tggaaaggga
      241 aaccagataa gtgattactt acttttcttg atatgcttca tcagacgctt acgtttacgc
      301 atctgggttg gcgtcagggt gttacgcttg ttcgcatacg ggttttcccc ttctttgaac
      361 tgaatacgaa tcggcgatcc cattacgtcc agcgatttgc ggaagtagtt catcaggtaa
      421 cgcttgtagg aatcaggcag gtctttcacc tgattgccgt gaatcaccac aatcggcggg
      481 ttataacccc cggcgtgggc gtatttcagc ttcacacgac gaccgcgtac cagcggtggt
      541 tggtgatctt caacagccat cgtcatgatg cgcgtcagca tagaggtccc cacacgacgg
      601 gtggagctgt catacgcttc acgtactgat tcaaacaagt taccaacacc actgccgtgc
      661 aaggcagaga taaagtgcac acgagcaaaa tcgataaagc ccagacggaa gtccagcgtc
      721 tctttcacct gctctttcac ttcctggctc aggccgtccc acttgttcac tacaatcaca
      781 agtgagcgcc cactattgag aataaagccc agcaaggaga gatcctgatc ggaaatacct
      841 tcacgcgcat caatcaccaa catcaccacg ttggcatctt caatggcctg caacgttttg
      901 attaccgaga atttctctac cgcatcggtg attttgccgc gtttacgtac gccagcggtg
      961 tcaatgagca catactcacg tccgtcgcgt tccattggaa tgtagatact gtcacgcgtc
     1021 gtgccaggca tgtcgtaaac aacaacgcgc tcttcaccaa gaatacggtt agtgagtgta
     1081 gacttaccta cgttcggacg acccacaatc gccagtttga tcggcagact ttgcgggtcg
     1141 aagtcgtctt cctcttcttc ttcgccgttc tcttccgctt caaattgcgc ccagtattcg
     1201 gcgtcttcgt caacttcctc ttgcggtgcg agatcttcca tccacggcag cagcacgtgc
     1261 tccagcagac ttaatacgcc acgaccgtga gacgcggcga tcgggtagat ttcacctaaa
     1321 ccaagcgcgt agaaatcaac cacggcctga tcgggatcca gaccgtcagt tttgtttgcc
     1381 accaggaagg tcggtttttc acgggagcgc agatgtttag caatcgcttc atccgccggc
     1441 atcaggcccg cgcgcgcatc caccataaac agtacgacgt ccgcttcttc aatcgccagc
     1501 agcgactgtt ccgccatgcg ggtttctaca ccgtcttctg tgccatcaat cccgccggta
     1561 tcaatgcaga taaactcacg gccttcaatt tccgcacgac cgtacttacg gtcacgagtc
     1621 agacccggga aatccgcaac cagcgcatct cgggtgcgag ttagacggtt aaataacgtg
     1681 gattttccta cgttagggcg cccgacaagc gcgaccacag gtaccatgtt taaagcctca
     1741 tttttataaa tcatcagaca acgcacgcta tattcgtgtc gttgttaaaa acaggaaaac
     1801 ggcccctgtc caggagccgt tttcaaagtg aacgacagag acgattaacg tgtaatagag
     1861 tacacggttc cgtcttttgc ctggatcagc agtttgccgt cagcagcaac cggttcagtc
     1921 tggaaaccgg aactatcaac tttttgctgg gcaacgaaac gaccatcttc gacgttaatc
     1981 cagtgcagat aaccttcact gtcacctacc accaggttgc cattatacag caccggagaa
     2041 gtcagcaggc gatgcagcag atcgctttgt gtccacagcg taacgccgcc atcaatggtc
     2101 aacgccatca cccggtcatt ttgatcgacc agatagatgc gattgccgtc gacgatgaaa
     2161 tcattcaccg aacccagttc gcgtttccac ataatctgac cactgcgcag atcaagcgcc
     2221 gtcaggttac cattataggc cagcgcgaaa acaacgccgt taacaacgac gggagtcgtg
     2281 tcaacatcgc tcagacggtc aatttcggta gaaccggtcg cctgggaaat acgctgctgc
     2341 caaatcatct ggccctgttc catcagcact gcgctgacgc gaccattatc gccacccacg
     2401 acggccgcac caaaagccgt tgccggcgca gactcgccac gcaaagagag cgaaggcata
     2461 tcgaggttaa ctgtccattt gacagcgccg tcagcttcgt tcagcgcttg taactgaccg
     2521 ttactggtgt ggattaacac cagaccgtcg ctgaccaccg ggcgcgaaag tgcttcaccc
     2581 gcgactttag tttgccatgc cacagtacca tcgctggtat tcagcgcgta aacctgcgcc
     2641 ttttcggtgc caatgtagac atgcccacca gacacggtca cgccgccaga aagtaatgca
     2701 ggatctttag agaaccagcc atctttctcg gccaggttga cagaccagat ttctttgccg
     2761 tcatccgcat tcagcgcttt tactaaacca gcgcggtccg ctgcatagac aacgttgtcc
     2821 gccagtgccg gatgaagatt ggaatagaag ttgccaatgc cgctaccaac ggaagtgctc
     2881 cacacagtag tcggcgtaaa ctggttttca acggttggca atggggacat ctttaccaca
     2941 tcttcttcgc tgttaaacag cgaacagccg cttaaaaggg taacggaaag cagtcctggc
     3001 agcagtaatt tacgcaattg catcgggtcc ctctcagatg gacaaattat taattttcat
     3061 ctgcatcatt tcgctcagtg ccggggtaac atcgcttttc acgcctgctt cccatgcact
     3121 acgcgcacct tgcttatcac ctttgctcag caatgcttca ccacgcaggt cggcaacaat
     3181 ggcagcccac ccttcacctt tgatggtatc aagggttttt aacgcggcat cagcctgctt
     3241 gagctgtacc tgaacgcgag caagacgcag atttatcacg gctttgagat tttcatcgct
     3301 cgtgtctgcc agcccctgtt gtaactgggc ggcagctttc tctagttcat ttttgtcaac
     3361 aaattgctgc gcaagttcca aagaagccag cgcaccataa gtatttttat tttcagcagc
     3421 aaatttttcc gccgctggga tgctatccgg tttgccttcg ctcactgcgg taaccgcatt
     3481 ttgataggca agagaagcgg agcgtgcaga atcaacctga tggctgttcc agtagcgcca
     3541 gccaatcagt gcgccaacgc ccaaaatcac cccaacagcc agtgctttgc cattttcagc
     3601 aaaaaagcgt ttaaccgctt caacctggtc gttttcgttc tcgtaaattt ccacgctgtc
     3661 cttctccttc cttaacccag taacgtgcgc aaatgcgcgg ctacgctatc ctgcgcaact
     3721 gcagtttgct caccagagcg caaatccttc actactgctg tgccgttagc cacttcagac
     3781 tcacccagca ccacagcaac gcgggcaccc catttatcag cacgggcaaa ctgtttctta
     3841 aagttgccgc cgccgtggtt ggtcatcagt ttcacgcccg gtaattcatc acgcagacgc
     3901 tctgctaatg ccatagccgc agattgtgtg tcagcacctg aagccaccag gtatatatcg
     3961 acaacaggat cggctttaaa ttccggatta acggcctgaa ctaacaatac aagacgttcg
     4021 aggcccatcg caaaaccgac tgccggtgtt gcacgaccac ccagttgttc caccagaccg
     4081 tcataacgac cacctgcaca cacggtgccc tgggaaccga ggctgttagt cacccactcg
     4141 aaaacggtac ggttatagta atccagaccg cgcaccagac gctggtttac ggtgtaagcg
     4201 atccccgcgc tctcaagcag tttgcacaga ccggcaaagt gctcacgaga ttcctcgtcc
     4261 agataatcgc ctaatgccgg agcgtcgttg agaagcacct gcacttccgg attttttgaa
     4321 tcgagtacgc gcagcgggtt agtgtacatg cggcgtttgc agtcttcgtc cagcttttct
     4381 ttatgctgct caaggaatgc caccagcgca tcgcggtaat tcgcacgagc ttccagcgaa
     4441 ccgatagagt tcagctcaag agttacgtgc tcggaaatac ccagcgcgcg ccaccagcgg
     4501 gcagtgagca taatcagttc ggcgtcgata tccggacctt gcagaccgaa aacttcgcag
     4561 cccaactgat ggaactgacg ataacgccct ttctgcggac gctcgtgacg gaacataggc
     4621 ccgatatacc acagacgctg ttcctgattg tacagaagac catgctcgat gccggcgcgt
     4681 acacagcccg ccgtcccttc agggcgcaga gtcaggctgt cgccattgcg atcctcaaag
     4741 gtgtacatct ctttttcaac cacgtcggtg acttcaccaa tcgcacgttt gaatagcggg
     4801 gtctgctcta caatcggcaa gcggatttca ctgtaaccgt agctgccgag cacgtttttc
     4861 agtgtgcctt caatgcgctg ccagatggcc gtttcgccag gcaggtaatc gttcatgccg
     4921 cgaatggctt gaatgttttt tgccacgttt attctctttc tgaatataaa aatgaaccct
     4981 caacgcttcc ctcaatgttt cgggagccat gcgggttcaa tcatacacgg gaagcgaggc
     5041 gcttcccatc acgttattat ttttcaacct gctgaacgtc aattcgacgc gcttcgtcca
     5101 gctgactggc tttcgcacga atgcgtgctt ccagctggtc gatcatatcg ttgttgtcca
     5161 gacggtcttt gcgcacgcca tcttcataga ggccgctttt cttgttgccg ccggtgacgc
     5221 cgagtgtaga aaccagcgcc tcaccagggc cattcaccac gcagccgata atcgaaacgt
     5281 ccatcggagt gatgatatct tccaggcgtt gctccagcgc gttaaccgta ccgataacat
     5341 caaattcctg acgcgaacag gtcgggcagg cgatgaagtt gatcccgcgc gaacggatac
     5401 gcagcgattt caaaatatcg aaaccgactt tgatctcttc gaccggatcg gccgccagcg
     5461 atacgcgcag cgtgtcgccg atgccttcag acagcagcag acctaaacca atagcggatt
     5521 ttactgcccc gctacgcgca ccaccggctt cggtgatccc cagatgcagc ggctgatcga
     5581 tttgttttgc cagcaaacga taagactcaa cagcgaggaa gacgtcagac gctttcacgc
     5641 tgactttgaa ctgatcgaag ttcaggcgat cgagatgatc aacatgacgc atggcagatt
     5701 ccagcaacgc ctgcggcgtc ggttcgccat acttttcttg cagatctttt tccaacgatc
     5761 cggcattaac gccaatacgg atcggaatgt ttttatcgcg cgcacagtca accaccatgc
     5821 gaatacgctc ttcattaccg atattgccag ggttaatacg cagacaatcg acgccgtatt
     5881 ccgctacttt cagcgcaatg cgatagtcga agtggatgtc agccaccagc ggcacgttaa
     5941 cctgctgttt gatgagtttg aacgcttctg ccgcgtccat cgtcggtacg gagacacgga
     6001 cgatatcagc gccaacacgt tccagcgcct tgatttgatt gaccgttgct tcgacgtctg
     6061 tcgtacgcgt attggtcatg gactgtacgg cgatgggagc accatcgcca atcggcacat
     6121 tcccaacgta aatacgtgtt gattttctac gttgaattgg agcctggtta tgcatgaaaa
     6181 atctcccgcg ttacccgtct gttactgcgc cggtgattgt tcggcattga gggtcagacg
     6241 cgcaacctgg ttagttctga taaaacgact cagatcgaca ggtttccctt gatactggat
     6301 ctgtactgcg gctggcgcac caattttcag tttgtacggt gcctggccgg ttaagttcaa
     6361 attaccgtct ttacgctgca taccgctaaa cagtttttta ccggtagcat cggtgacttc
     6421 cagccagcaa tcggcagtaa agttcatcac cagcgcattc gggtcaaccg ccggcgtggt
     6481 cacgccagcc tgatcggttg gcaacggcgc agcgccatct ggtgttgttg ttgccgctgg
     6541 tgccggagtc gcagcggtat caacatttgc ctgcgaaggt ggaacaaccg cattctgttg
     6601 cggatcaaca gctggtgctg gcgcagttac ggcaggtgtt tgcgtgttgg ttgcggtggt
     6661 atccacaggt gatgtcggcg tggtcgccat agcctgatct gtggttgtcg acgtatctaa
     6721 cggtacactc tggctttgat tattattcag ttccgcagaa gattgatcgg ccatagtggt
     6781 gatctcttcc tgctgagctt tgtggtcttg ccaccaccag gcaccgctca ggccaataac
     6841 cacaaacaat accagccagg tgaaggtcat cagccagccg tcacgttttt tgcggcgttt
     6901 accgagggaa aaactctgca tcggcgcaac ttttgcagcc cgtagtggag cctgcttttc
     6961 cagccctggc agcagttctt cttctggaat atgtaccaga cgcgcataag agcggatata
     7021 tccgcgcagg aatgttgaag caagatcggc gggtgcctta tcttcttcaa tgtcgcgtac
     7081 cgtggaaacc ttcaggcaaa gtcgctcggc aacggcctgt tgactaagtc ctagttgttc
     7141 gcgagcatta cgcaggcgag cgccggtagt aagtgtttca ttttggtcgt gcgtggcttc
     7201 agtattcatt cgctgcaggt acgtttaaat gagaattgag atgccggtga accatcatgc
     7261 ccacccacac cgcgaaacat ccgttaagtt aaccgttatc atacagtata agaccgtctg
     7321 tgtgctcgtg acaaagccca cattcgctaa ggctaactta ctgttgcatc gttacatact
     7381 gccttaaagt cagcaaaaac gcaccgttaa tattgaccag acaattgcaa cttaatactt
     7441 cattcataaa agattacgcc acggtacata aagtaaccgt ggcgtaatgg ctatcagacc
     7501 gctttaatgt cgatggcttc accctgcatc cgcttacgca gagtacgttt cgtacggtcg
     7561 ataacatcgc ccgccaactg accacaagca gcatcgatat catcaccacg agttttacgc
     7621 acaatagtgg tgaaaccgta gctcatcagc acttttgaga aacggtcgat acggctgttc
     7681 gaactgcgtc catacggcgc gcccgggaac gggttccacg ggatcaggtt gatcttacac
     7741 ggcgtatctt tcagtagttc cgccagttgg tgcgcgtgtt cagtgccgtc gttaacgtgg
     7801 tcaagcatca cgtattcaat agtgactcgg ccctgattgg cgttggattt ctccagataa
     7861 cggcgcaccg ccgcaaggaa cgtttcgata ttgtactttt tgttgatcgg cacaatttcg
     7921 tcacgaattt cgtcgttcgg cgcgtgcagg gaaattgcca gcgctacgtc gatcatatcg
     7981 cccagtttat ccagcgccgg aaccacaccg gaagtggaaa gcgtgacgcg acgttttgac
     8041 aggccaaaac cgaaatcatc aagcatgatt tccatcgccg gaacgacgtt gttcaggttg
     8101 agcagcggct cgcccatacc catcatcact acgttagtga ttgggcgctg accggtgact
     8161 tttgctgcgc cgacaatttt cgccgcacgc cacacctggc cgataatttc cgatacccgc
     8221 aggttgcggt taaagccctg ctgggcggtg gaacagaatt tacactccag cgcacacccc
     8281 acctgcgaag agacgcagag cgtggcacgg tcgtcttccg ggatatatac cgtttcaacg
     8341 cgctgatcgc caacggcgat cgcccattta atggtgccgt cagatgaacg ctgttcttca
     8401 accacttccg gtgcacggat ttccgccacc tctttcagtt tgccgcgcaa cactttattg
     8461 atgtcggtca tctcatcaaa gttgtcgcag caatagtgat acatccactt catcacctga
     8521 tcggcgcgga agggtttttc acctaaatct ttaaaaaact cccgcatctg ctgacggttg
     8581 agatccagca ggttgatttt tccatctttt gtggtgacgt tttcaggtgt gactaattgt
     8641 tcagacatat gctattccgg cctcgttatt acacgttatg gcccctggag ggttgaaaaa
     8701 agaaacgccc cggtgagcgg ctgctcgtcc gggggcgctg cattgtacaa attctggcgc
     8761 acggatgcca cgtttgcacg cggcatttac gaaattatta acgggtgcgc gggcacactt
     8821 cgccttcgcc gaagaaataa gcgctttcgc gagcggcaga ttcgacggaa tcagaaccgt
     8881 gggtaccatt ttcggtcagg ctgtcagcgt aatcagcacg cagagtaccg gccagtgcat
     8941 ttgccggatt ggtcgcgccc agcagatcgc ggtgacgctg aacagcattt tcaccttcca
     9001 gcacggaaac aacgatcggg ccagaagtca tgaattcaac cagaccatca aagaacggtt
     9061 ttccatcgtg ttcagcataa aagccacgag cctgttcaac ggtcaggtgc agcattttgg
     9121 tgccgacaat tttgaaccct gcagcttcaa agcgcgcaaa gatactacca atcacgtttt
     9181 ttgctaccgc gttcggtttg atgatggaaa aagtacgttc aatagccatt tttacctctg
     9241 taaattgttc tgttgttgtc tgtaccagcg tacgagatgg cgcggattat aatgagcaac
     9301 agggccgttg actattgatg aaggtaacat ttttttaaaa taaacccagt ttaagcaaca
     9361 atcagggaca aaacagacta ttgcatgaca aatttcactg tcgctatttg ccccacgtca
     9421 tccatcacca gcaattgata atcgccttta tccgtcaaat gcagggtcac gttgcgccca
     9481 cgttcagtta acggttcgcc attcagaaac caccagcgtt cacctgcccc tccacttgat
     9541 tgcaacggca aagtcgcttc cgctgcgccc ggtaaacgtt taataatcgc gccatcgcgc
     9601 acacctgtta gctgcagcgg gagttgtgca tcgtgaccgt atggcgggca aattgtcgag
     9661 gccggtggca agcgcacagc gcgacgttct gatgcgggca gccagggttc cagcggtagc
     9721 ggccagacat ttatcatttc ttgtcgcgcc tgagggcaat cagcggcaac acgtttgccg
     9781 ttttcatcca gccagatggg gaaacgaatg ccattaatgc cttcctgctc cggcagtaat
     9841 agagttggcg gctggctccc gtccagcagc caggttgcca ggcggcggcg acagttaccg
     9901 tcaccttccg gcaaagactg tccgcccggc cagcagataa cgccacgact tacagagtca
     9961 ggacgtgggt cttccggaaa attcacactg cgcgacagta agatattatt aacctgattc
    10021 aacagcggta tggcactggc aaagccaaac tgaccaacaa cgggcgtgcc gtccggtctg
    10081 ccagtccaga tcccaatgac atagcgagcg ttaaccccaa tcgcccaggc atcacgatag
    10141 ccatagctgg tgcccgtttt ccatgccagt ggggcgacgc gcggcaaggc accatccggc
    10201 aagggttgcg cttcatcagc cataatccgg cgaatgatcc acgcagcccc cgacgacatt
    10261 aaaggccgtt caagcagcgg atcgtcaggc tgtaagcgca atttacctgc cttgccgtgg
    10321 cgagcaaacg cggtataggc cgccgccata tcttccagtt ttgcaccagc gccgccgaga
    10381 atgagcgaaa gattcggcgc agcaccgttg ggcagataca acggcaatcc aacattgcgt
    10441 aattttgccg caaaccgttt cggtccatag gcttccagca cctgcacagc tggtaaattc
    10501 agcgagcgca ccagcgcctc gctcatgctg atcggcccat gaaaaccgct gtcaaagttg
    10561 ccaggtcggt aatcaccggt acgccggggg acgtcttgca acagagatgc cgggtggatc
    10621 aagccttcat ccagcgccag accgtaaaca aacggtttga gcaccgatcc tggcgatcgg
    10681 atcgcattga ccatatcaac atgaccaaag cgtgaatcat cgttgagatc aaccgaaccc
    10741 acccagccgc gaacacgcat atcggtatga tcaaccacga tcatcgccag tgagctgcgc
    10801 ggtggtaatc gtcctttcca gttttgcgcc agttcttcca gacgtcgttg aagaccggca
    10861 tccagcgtag taacgatttt gtcgcttttg cttttaccga gcatcatgcg cgaaaacagc
    10921 ggtgccagtt gcggcatttg tcggggtgcc agccagatgg gttcttccct tgactctttt
    10981 acccgctcat gggaccacac accttgcacg gccatccgtt cgagcacttt attacgcgcg
    11041 gcttcggcac gctccggcca acgatccggg cgaagacggc tgggcgcttg cggcaaaacc
    11101 gccagcattg ccgcctcgga atagcttaaa ttcgcaggcg atttcccgag gtaagcccaa
    11161 cttgccgcac caatcccctg caacgtaccg ccaaacgggg cacggttaag atacaaggtc
    11221 agaatttcac gtttagacag atgccattcc agttgcaacg cgcgccagag ctggcgaatt
    11281 ttgccgccaa acgttttggg gtgaggatca agcaggcgcg ccacctgcat ggtgagcgtg
    11341 ctgccgccgg aaatgacccg acccgacgtg agatcttgcc aggctgcacg cgccaccgag
    11401 aacggattca cccccggatg cttccagaac cagcgatctt cataattgat cagcgcttca
    11461 aggtaacgtg gagaaacatc ttcgattgtt accggataac gccagatgcc gtcagcatcg
    11521 gcgaaacgcc agagtggcgt accatcctgc gccaccacca cgcgtgcagg attcacttct
    11581 tgcaatggca gaggccagag tttatctgct gcccatgctg ccagaatgat gataaaaggc
    11641 gcggctgcca gcattatcca gcagccgcgt ttggttaaca ggcgaggcat ttacggtctg
    11701 actatcaaca gatcgtcagc cgcaccagtc gcccgccatt gaggaacata cattgattcc
    11761 accataggct gcggcacctg atacgttccc ggcgtcaccg cccgcgccag atacaccagc
    11821 gttaccggtt gatattcatc aacggcaacc gccgccacaa agcgatcgtc acggaactct
    11881 atgtgcttaa tgctcgcctg ctgcatctgg ttcagcaagt tctgcacttc accaccactt
    11941 tgctccaggc tggcgctgcc gttcgccaga ttctggtttt ccagttccag ccccgcaggc
    12001 aacagatcca cgactaacgc atccggtaca ctgttactgg ctttaacctg caaccacacc
    12061 agcaccagat cgccgctacg taacgagtcc agcgatttgc tcttaccgtc agtacccaga
    12121 atatgacgct caatttgcag cacattgctc gccggtaaag gtgcggattg cggataaccg
    12181 ctggcatcca cacgcagcca taacggctga tcgccactgt tggtcacctg caaggtggca
    12241 agttgatcgc tattcagatt gctggtttgc gctttctcgc ctgtcagcgg ctcagcagag
    12301 aaagtggttt gcgcctgcca tttgcccggt aaatcctgaa gagtacgggc agccaggaac
    12361 aacgcgttac tttcctgcgt cgatagccag cgttcaccga acgcctgctg cgaaagtgtg
    12421 ttcaacaggg agtactgctc atcgggtagc agtttgttct cttccagcaa tgagagcatc
    12481 aacgcgctgt cgcgcagtgg actaccgtaa tcacccagcc atatccgctc atcactatta
    12541 cgcggcgttt tcagcgccag cacaatcgct tcttcaccac gcattgcatc acccatggtt
    12601 ttcagcgcaa cgccaagttg cagcagcggt aaaccagaag cggcatctgc acgatgctcc
    12661 cagatttcac gcagcgcacc cagtggtgct ttttgctgac gggccaacac cagcgcagcg
    12721 taagactgta cggcgaattt actggctttg agattatcag cgtacgggat cgatatcatg
    12781 cccggatctt gtaaatagcg cagcagacgc tcattacccc ggttaatggc gtctgtcggc
    12841 acgctgtaac cctgttcgcc tgcgcggacc aggaaatcca tcacgtaagc cgtcagccag
    12901 tactcttcgt caccgttttt atcccacagc gcaaagccgc cgttatcacg ttgcatttgc
    12961 agcaaacggg aaatgccgat atcgaccgat gcacggcgtt tctcatcact gtcgcctttg
    13021 atgcccaacg cctgcagttg ggctgcattg gtataaagtg acgggaacag gccgctggcg
    13081 gtttgctcga gacagccgta cggatacgct tttaactctt tgatataacg tgcgatgttc
    13141 agcggtggtt tgccgctcaa caacaattgc ccttccagcg taacaggcga gaagttttgc
    13201 aatccatcag ccgggagcgc ccaggtttca ccaggctgta acgccgtacc gtaattgacc
    13261 gtttgtgccg ggaatgccgg acggacgccg attttccact gcttatgctg atcggcaacg
    13321 gtttccccgg gtaacgctaa cccgctaatg gtggcctgaa tgtctccatc gccataaccc
    13381 ggcaatgcac gcaccgggat aaacagcgta gtacgcaccc ctggcgctaa ttcaacaggt
    13441 gcgggggaat tactcaccag ttcaagcaag ccactggcgg tcagagcaac attaagtttt
    13501 tgcggtttat cggtaagatt agtgatatcc agcgtcaggc gcgaggtatc gccactcgcc
    13561 ataaagcgtg gcatgttcag ttcagcaatc accggtgcag caacaatcac tttgctttcg
    13621 ttgcttccga agtcatctgc cgtccaggct tgcgccatga cgcgcaattc accgttaaaa
    13681 tcgccaattg gcagtgtaac cgagccttcg ccctgttcgt tgagcgttac cggcagcgcc
    13741 tgctgcgcga caatattgac gtgattgacc ggcggtttac caccacgttt cagctcatca
    13801 ccatcaccac caaaacgcag agctgccaga cgcccctgac cttcaatgac ctgaccgtaa
    13861 atatcgtaaa tgtctgcgcc atagcgtttc tgaccaaaga acgcctgcca cggatctggc
    13921 gtgacgtaat cagtaatatt cagcacaccg ctatcaacgg cagacaccag cacattcacc
    13981 tgttttggca tctcaccatt tttattgctg gctttaattt tcacggttaa tggctgattg
    14041 gggcgcattt tggtaggggt ttccagcgcc agatcgaggc gacggttttc atcaccaagc
    14101 ggcagatgca acaccccaac cgcgcgtttt ggcgtcgcgg agcgagattt atcgccagga
    14161 cgtaccacca gcgtacttaa atacagatca tgacgattcc aggttttatc gaccggaatc
    14221 gtcagatcca gcccttgagc cggaacatca atctcttgcc accacagcgg cccttcactg
    14281 gactcgacca tcgcataacc tttacccgcc gttggcgcgg caatatgcaa cttaatggta
    14341 tcgccagggc gatagctggc tttatccagt ttcagcgtga cacggtcggg tcgcactgca
    14401 ccacccccgt cgctgttatc ctgccagcta taaccagccc agaaacgcac actactgacc
    14461 gcttcattcg gcgctttaac ttccagacga taagcgcccc actctaccgg gaaactgacc
    14521 ttaccggttt cgtccgcttt cagatccaga gtttgttcat tttcgatcag atctttttga
    14581 tcaaactgtg actgccagcc ttcatattcc gaccagttcc agtagtaatc gcggcgttcg
    14641 cgaatcaggc gcacctgcaa gcccgacacg gcttttttca cgccttgcgc atcgctataa
    14701 acgatgtcaa aagcggcgtt actgccttca tcaacaatgg gctgtttcac cgtgctgtcg
    14761 gtgcgataat cgtaaaccga tttcgaggcg aactgcggac ggatccccgg caatgcatcg
    14821 gcaggccaga tagcctgctc agcacggcgc gtcaccgggc gaccgcccga ttccagcaaa
    14881 ctaccctgga aaataacctg taatggggaa tgcgtttcct tccactggct ttctgtagaa
    14941 acttcgccgc gccctttatc atccaacgtc aactgaactt catccagcgt gcgggaaaga
    15001 ttttcggcag ctatatctcc gaattcaaaa ccaggtaagg ctgacacagc ttcacgcagt
    15061 gggcgcagga aaagttgccc ttgcaaagta ttaccattag caggtgcacc atacaggtag
    15121 taccccacca cggagaattt cacttcatct ttcggcgtta gcggggtttt ctcaccggtc
    15181 agattcagcg ccatacgctc tggcataaaa tcttcgacgt ggaaatccca catccgatac
    15241 tggttatcgc ccgtgttagc gcgaatatgc cacataccgg ttgccgcatt gctatcgagt
    15301 ggccaggtaa agtggtagag gccattctcc ggctgactaa cgacgctcct gagtacctgc
    15361 ccgtcgggtt taatcacgtc cagcttgaca ggttgatcgg gcaacgcttt accgtctgca
    15421 tcacgcagca aaccattgag gatgaccgtt tcacccgggc gataaagatc gcgcgggcca
    15481 aacatgaaaa actgtttgct atagcccggc gcgccagcaa tgttaaattc cgctaagtcc
    15541 agcgccggga gttttaaatc gagcagcgtt gtctgaccgt ttttacgcgc cagcagtaat
    15601 gccgcgtttt tatcattttc cagctgcaca tgcccctgag cgtcactggt tgcctgagtc
    15661 agagtctgcc ctttctcatt taataaagag acttcaattc cttgctgggc cgcgccgttt
    15721 tccagacttt gggtaaagat atccagacga ttgtgataac ggtgagctga aacgccaata
    15781 tcacttaacg taaacagcgt cgcgggatta ctgtaatcgt aacgtccagc ctgattcatc
    15841 acagccagat acacgcccgc ctgctgaagc ggtttgatat cgcccagcgg cagcaataat
    15901 ttttcacggg tgttacgcgc aggattgaga tcaaaccgtc cggtgtagac cagatccgcc
    15961 atctgcagca gtttatctga ctgccagttc gccagcgaat tgcggtattc ccactggcta
    16021 atgaaagcgg gcagtgattc tggtttcaca cggaagaagt taacatcgac attgttaacg
    16081 ttaagcgcca ttaccggcag cccttcaacg accttaccag gaagcaacga accacgactg
    16141 gcaaaaccga cgctgggttg gatatcgcgg gtagttatag ttttttcgtc atctttacta
    16201 aaggttgcgt tattgagcgc tttgacctcc ttgccaatag tgacgatcaa atcacgtttc
    16261 ggttcgaggt ggcgtaaacg cagctcttta agattatgtg acagctccca ggcaccatcc
    16321 actttgccgc tttttttatc gacgacatga ataacgcgtg agaaatcctg atccgggtcg
    16381 agagggatgg agaacgtcag caccagcgtg gctgcgccat cgagttgaac ttcagagaga
    16441 tccagcaatg tcagcgcctt accggcactc tgttgggcca gtttttgtcg ctccggcgcg
    16501 gaaagttttg ctgaactcac gttttctgaa gaggccgctt tagtaacttc agaaggcgca
    16561 tcttttttca ccgttgttgg cgcgttatcg ttgttgtcgc accctgccag cgccagcatt
    16621 agcatgcagg cggctacgcg taactttttc atttttcatc cctgccacaa tggcccgtta
    16681 gcaacgtcga ataattatta tgcgtgagaa tttacgttat gtaattcagt atcacgccca
    16741 tacaatccga attttagtga gaattgtggc tcagacagcc aaatcagcct cttttgcttg
    16801 tcgcttttca ctaaacatcc gacaatttag cctaaccccg gcaaaaatgg agatgcctat
    16861 gtccacgaca tggtttgtag gagccgactg gctcgccgaa catattgatg acccggaaat
    16921 tcagattatc gatgcccgca tggcgtcgcc tggacaggaa gatcgtaacg ttgctcagga
    16981 gtatctgaat ggacatattc ccggcgcagt gttttttgat atcgaagcgc tttctgatca
    17041 cacttccccg cttccgcaca tgctgccgcg cccggaaacg ttcgccgtgg cgatgcgtga
    17101 attaggcgtt aaccaggata agcacctgat tgtctatgac gaaggtaatc ttttctcagc
    17161 cccacgagca tggtggatgc tgcgcacctt tggtgtagag aaagtgtcaa ttctgggtgg
    17221 tggacttgca ggctggcagc gtgatgatct gctattagaa gaaggcgcag tagagctgcc
    17281 ggaaggagag tttaacgccg cgtttaatcc tgaagccgtg gtgaaagtaa ccgatgtatt
    17341 attggcaagc catgaaaata cggcgcaaat tattgatgcc cgcccggcta cacgttttaa
    17401 cgcagaagtt gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa
    17461 cgttccgtgg acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc
    17521 gatatttttt ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg
    17581 tgtaacagca gccgtggttt tgttggcact cgcgacgctg gatgtgccaa acgtgaaact
    17641 gtacgacggc gcatggagtg aatggggcgc acgagcagat ttaccggttg agccagagaa
    17701 ataagctttt aacaggcaat aaaaaaccgc cgaatttggc ggttttttat tgctggtctg
    17761 gttcgcggcc tttccagcag attggcttgt gttgcatgag caacgcaggt gcttcacagc
    17821 aaagcaatac tcgccagtaa ctctcttttt gtcaagcgaa agagagtaat cattgtttat
    17881 ttagcgtatt atcgacaccg gccctttccg ccgtctcgca aacgggcgct ggcttaagga
    17941 aaggatgttc catggccgta aatgcaggtg tttcacagcg cttgctatcg cggcaatatc
    18001 gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga tacggtgctt
    18061 ttgtatcgta cttattgttt ctggtgcgct gttaaccgag gtaaataata accggagttt
    18121 ctccggcaac aatttactgg tggttaacaa acttcagagc agcaagtaag cccgaatgcc
    18181 gccctttggg gcggcatatt ttagattatc cggttctgtt taaagtcacg caaaaaacca
    18241 ccccagcgac gttcatagaa tggcgcaata tgttcggtaa taaagtggct aattcctttt
    18301 tccccttttt tcacctgaca aatatcgatg ggttcatcgc caggtaatgt atcggtcgct
    18361 acacttcccg ccgcctgaat aatttcttcg atatcaccat cggcttcaat gccaataagt
    18421 aaattaggct gtgcctcttc gctctcttta attaaacaaa taaaagcacg cttcactggc
    18481 ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtactggcgg ctctgcgact
    18541 tccgataata tcagcgattc accgccttcc agtacttcct ggctactcag cggattccct
    18601 tcttcaccaa tcaacaaact gatttcacgc ggcataaact ctttaccggt tggcagtttg
    18661 gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac gggcattacg
    18721 acaaatgcct gttcgtcttc aaccgcctgt tgaagtgctt ctaacgaggt aaaaaaagga
    18781 atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcgct atcttcaact
    18841 actgcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag tagagtacgg
    18901 aaaaaggccg ggcggtgagc cggttcggtt gctgcttttt ccagcaggtc ttcaagttcg
    18961 ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggagca agtgcctgat
    19021 gcgccacgct tatcaggcct acaagggagt cgcaatctat tggattttcg caatttgtag
    19081 gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa cagattagca
    19141 atcgtgcgca caccaagtcc cgtagcaccc gcagaccact gttcaaccgg cgctttacgg
    19201 taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac aaagtgcgac
    19261 aggaagcccg ccgctgtgct cgcgcctgcc ggatacgccg cgcttccggt gttgttcagt
    19321 tcggcaaagt tagacggcaa ttgattgcgg tggaactccg ccagcggcag acgccagaac
    19381 ggttcattct cctgcgcggc actcgccaac aagcgaccgg caagcgcatc gtcaaaactg
    19441 aacagcgcgt gataatcatt acccagcgcg gttttcgccg ccccggtgag ggtcgccgca
    19501 tcaatgatca gttccggttt ctgcgcactg gcatcaatca ggccatcggc cagcaccaga
    19561 cgcccttcgg catcggtgtt catcacttca acttttttac cgttgcgata ggtaataata
    19621 tcgcccagtt tgaacgcgtt gccgctgatc aggttatccg cacagcagag gaacagcttc
    19681 acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac cgttgccgcg
    19741 ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct gtagccgccg
    19801 gaatcaaaag tgatgccttt accaaccagg cacgcgtaca ctggcgcttc tttatcgcca
    19861 gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg cccgaccgtg
    19921 tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg gtaagtcaca
    19981 cgatcccctg cgacgttgct gatcagatca acagcacgct gtgccagttg cgatggcccc
    20041 agctcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat caggcggtta
    20101 tccagttcct gacgctgggc atcgtccaga tccggccact ccactttacg tatgcctttc
    20161 ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc ttcaccgctt
    20221 aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg gatcaacccc
    20281 agatcgtctg ccccgttcaa atgcagggta atgccatcat tattaatgct gtaagtcgct
    20341 ttttctcccc aacgtgcgtc ggcaggttgg ggagagaggg taatcttcat cgcttctgtc
    20401 attttagtta tccttattcg tttaacagca aaacgggcta cccccaggca gcccgttcat
    20461 tcactcggct tcgtccagcc agaccaacaa aatcgcttcg aggattttct cgttcgatgc
    20521 ctgcgggtcg tcgtcgaaat cttccagagc gcaaatccac tgatgcatat cggtgaatcg
    20581 aaccgttttc ggatcaatat cgggatacgc atcgtacagt gcttcgccaa tttcgcggct
    20641 atcggtccac ttaagtccca tactaacctc tgttaatgct cacgcgcatg gttgatagtg
    20701 taacgcggga tttcaaccac taaatcttcg tcggtgacgc gcgcctggca gcttaaacgg
    20761 ctttccggct ccagtcccca ggctttgtcc agcatgtcgt cttcctgctc tgagctttcc
    20821 ggcagcgagt caaaaccttc acgaacgatg cagtggcagg tggtgcaagc acaggatttt
    20881 tcacaggcgt gttcaatctc gataccgtta cgcagcgcaa cgtcgagaat ggtttcaccg
    20941 ctattcgctt ccagaacagc gccatcaggg cagagatcct gatgaggcaa aataacaatc
    21001 tttggcatat taaacctcgt ccacggaatg gcctttcagc gcacgacgaa ccgactggtc
    21061 catgcggcga gcggcgaaat cctgggtttg tttgtctacg tttttaatcg cttgttcgat
    21121 ggcgtcaaca tcatcgccct gcgccacttc actcaggtga gcggcagcat cgtcgataac
    21181 ctgacgttct gcggcgctta acagcgcggc atcagcagcc agcgcaccgt gcagactttc
    21241 cagcacacgc gccgcttcta ctttttgttc tgccagcatt cgggctttta cgtcctgctc
    21301 ggcatagctc attgagtctt tgatcatcga ggcgatttcg ctatcggtca ggccataaga
    21361 cggtttgacc tgaatagacg cctcaacgcc ggtggatttc tccatcgccg tcacgctcaa
    21421 aagaccgtcg gcatcgacct ggaacgtcac gcgaatatgc gcaccgccag ccggtagcgc
    21481 cggaatacct cgcagcgcaa aacgcgccag cgagcggcag tcctgcacca gttcgcgctc
    21541 accctgcatc acatggatag acatcgccgt ctgaccatct ttaaaggtgg tgaaatcctg
    21601 ggcgcgggcc accggaatcg tggtattacg cgggatcact ttctccacca ggccgcccat
    21661 cgtttcgagg cccagcgaca gcgggatcac atccagcaac agcatttcgc tgtccggctt
    21721 gttacccacc agaatatccg cctgaatcgc cgcgccaata gcgacgactt tatccgggtc
    21781 gatagaagtc agcggtggac gaccgaaaaa ttcgcctact cgttcacgca ccagcggcac
    21841 gcgagtagaa ccgcccacca tcaccacttc cagcacttca tcagcttcta cgcccgcatc
    21901 tttcagcgcg cgacgacaag ccagtaaggt tcgttttacc agtggcgcga tcagttcatt
    21961 gaattgttca cggctgattt cgccctgcca gcccgcaacg ttaacggtca cggagaccgc
    22021 atcgctcagc gcgattttgg ccgcaatggc agcatcgagc agttcacgct gaacgcggtt
    22081 atcgctacga tcaggaatgc ccgcctgctc gcgaatgtaa tccgccagca gatggtcgaa
    22141 atcatcgccg ccgagcgcgg aatcaccgcc ggtcgccagc acttcaaaca cgccgcgact
    22201 taagcgcaga atggaaatat caaacgtccc gccgccgagg tcataaacag cgatcacgcc
    22261 ttcctggccg gaatccagcc cgtaggcaat agccgcagcg gtcggttcat taagtaagcg
    22321 cagaacatgc agccccgcca gacgcgccgc gtctttggtg ccctgacgct gggcatcgtc
    22381 aaagtacgcc ggaacggtga taacgacacc atccagctcg cctgccaggg cttcagttgc
    22441 ccgcgccgcc agtgctttga ggatgtccgc agaaacgcgc accgggttca gcagccccgc
    22501 cgccgtttca atcatcggca ggccattttc gctggcctgg aattgataag gcagatgcgg
    22561 atagcgttgc tggatatcag ccagcgagcg ccccatcagg cgtttaacag aactaatggt
    22621 gttggcggtg tcgagcgctg cgttagtacg cgcgtcataa cccaccgaat gcccttgctg
    22681 ttgatagtga acaacagatg gcagcaggtg acggccttca tggtcagcta acgtttcggc
    22741 ctgaccgctg cgcactgttg ccaccagcga gttggttgtg cccaggtcaa taccggccgc
    22801 caggcgacgc tgatgcggcg cagcactcaa accaggttca ctaatttgta ataaggccat
    22861 gtttagcttc cagaaattaa aaatcgagca gtttttcttc gagttgttcg gcactgctcc
    22921 gcagtttatc gagaaaacgc agcttacgca cggtatccgc cgccgcgtcc cacgtctcgt
    22981 tgtctaactg ttcaaccatc aactgatggc gggtatcaaa catctttttc acacgtttga
    23041 taaagctttc cagccgcgct tcatctttcg cctgttcgat ctcgtccagt tcttcgcgca
    23101 gttccagctg ttccatcagg aacgcggtgt cgcgcacagt atgctgctcg ctggcgagat
    23161 caaaaccgtg caaagaaagc aaatattccg cgcgcattaa cggatgacgc agcgtttgcc
    23221 aggcctggtt aatggtcgcg gattgctgta cggcggcgag ttgttccgcc tggcttccgc
    23281 tggcgaattt atcaggatga tactgacgtt gtagatcctg aaaacgcagg ctcagcgcct
    23341 gggtatcgag ttgataacgg gcaggcaagc caaagagggt gaagtaatcc ataacattct
    23401 cagggctgcg atccgccgcg ataacggcga atcaggtaaa acaaacccca cgcgcaggcg
    23461 accacggtgg ggttatcggt atgcgcatca aacgtggaag ctttcgccgc aaccacactc
    23521 atctttgacg ttcgggttgg tgaatttaaa cccttcgtta aggccttctt tgacgaagtc
    23581 cagctgcgta ccgtccagaa attgcaggct tttgccatcg accacgactt tcacgccttt
    23641 gtcttcaaac acgatgtctt ccggcgtcgg ttcgtcaaca aattccagta cataagccat
    23701 acctgaacac ccggaggttc tcacgcccag acgcaggcca aaccctttac cgcggttagc
    23761 cagaaaggta tttactcgcg ctgctgcact gtcgctcagt gtaatcgaca taaccaaacc
    23821 tcaactctta ttttgcttca cgtttgcttt tatagtccgc aatggcggct ttgatcgcgt
    23881 cttctgccag aatagaacag tgaattttca ccggcggcag ttcaagttct tcagcaatat
    23941 cggtgttttt gatcgcctgc gcttcgtcga gagacttccc tttcacccat tcggtgacca
    24001 gggagctgga agcgatagcg gaaccgcagc cgtaagtttt aaaacgcgcg tcttcaatga
    24061 taccttcatc gttgacttta atctgcaact tcatcacgtc gccacaggcc ggtgcaccca
    24121 ccattccgct gccgacggtc tcgtcgttgt tgtcaaagga acccacgtta cgcggattct
    24181 cgtaatggtc gataactttt tcgctgtaag ccattataaa ttctcctgat tccgataccg
    24241 attaatgatg agcccattcg atgctgttca gatccacgcc ctgcttgtac atttcccaca
    24301 gcggagaaag gtcacgcaga cgaccgatgg atttacgaac taactcgatg gtgtagtcga
    24361 tctcttcttc agtagtaaaa cgacctaaag agaaacggat agagctatgt gccagctcgt
    24421 cgttcagccc cagcgcgcgc agcacgtagg acggttcgag gcttgctgac gtacaggcgg
    24481 aacctgaaga aaccgcgagg tctttcagcg ccataatcag cgactcacct tcaacgtagt
    24541 tgaagctgac gttgagaatg ttcggcgcac cgtgttccag gtcaccgttc aggtaaactt
    24601 cttcgatatc tttgatgccg ttccacagac gattacgcag gccgcgcaga cgttccatct
    24661 cggtcgccat ctcttctttt gcgatgcgat aggcttcgcc cataccgacg atctggtgaa
    24721 caggcagagt gccggaacgc ataccgcgct catgaccgcc gccgtgcatt tgcgcttcga
    24781 tgcgtacacg cggtttacga cgcacataca gcgcaccaat acctttcggg ccatagattt
    24841 tgtgaccgga gaaagacatc aggtcaactt tcaactggct cagatcgata ggcagtttgc
    24901 ccacgctctg ggttgcatca acgtgataga taatgccacg agcacggcac atttcgccga
    24961 tagccgcgat atcctgcacc acgccgattt cgttatttac gtgcatgatg gaaacaagga
    25021 tggtgtcatc acgcatcgct gcttccagtt ctttcaggtc gataatgccg ttacgctgcg
    25081 gtgccaggta agtgacttca aaaccttcgc gctccagctg acggcaggta tccagtaccg
    25141 ctttatgttc ggttttactg gtgatgatgt gcttgccttt tttctgataa aagttggctg
    25201 cacctttgat cgccaggttg tcagattcgg ttgcaccaga ggtaaagacg atttcacgcg
    25261 gatcagcgcc gaccagatcg gcaatctgat tacgggcgat atctaccgct tcttcagcct
    25321 gccagccgaa acggtgagaa cgggaggccg ggttaccaaa ggttccgtcc atcgtcataa
    25381 actgcatcat tttctcggca acacgcgggt ccaccggcgt ggttgcggag tagtcgagat
    25441 aaatcggtaa tttcattgct ctataaactc cgtacatcac tcaatgcaag gaatcaggct
    25501 accggctgga tgtacgaccg tgtttacgga gtatttagca ctccggcctg attctgaatt
    25561 ctttttatta agcgcgtaac ttaacgtcga tcgcgtcttg tgtgcgggtg cgtggcgcgt
    25621 cgtgagtatg ctgacgacca gacacatcca gcacttcctg gttattaacc agttcgccta
    25681 aagtaatgtt attgagaaaa ccggtaagac ggtcgctcaa atcacgccac agcgcgtggg
    25741 tcaggcattt atcgccgccc tggcagccgc ctttaccctg acaacgggtg gcatctacag
    25801 attcgtcaac ggcgctaatc acttcgccaa cggcgatgct gctggcatct ttgcctaaca
    25861 gataaccacc gcctggtcca cgtacgctgg aaaccagacc atttttacgc agacgggaaa
    25921 acagttgttc cagataagaa agggaaattc cctgacgttc ggaaatatca gccaacggta
    25981 ccgggcccgc ttcagagttg agcgcaacgt caagcattgc ggtcacggca tagcgccctt
    26041 tagatgtcag tctcatgtct tacttcacct caaactcgcc cctgcccggg gttttttatt
    26101 gtaaaagtgg gggtattgca tagcagggtc aagtctgaca ttcccgagta aattggtcaa
    26161 ctatttactt gactgattta gtcgggtatt taaccttcag tgccattttt ttatcgtggc
    26221 gtttgtacct gttgtcggat gcggtgtgaa caccttatcc gacctgggat tccgtgcact
    26281 tacaggcctg ataagacgct ttaagcatcg catcaggcaa ccactgttat tcggccttgt
    26341 tacctttatt ctgctgctca atagaagcca gaatcccgcg caggatattc aattcctggc
    26401 tttccgggcg tgcacgggta aacagacggc gcaatttatt catcacctgc cccggatggt
    26461 tttcacggat aaaaccggtt gccagcagcg tttgctccag atgaccataa aaacgctcca
    26521 gatcatcgac cagcggatac ggcgtctctt catgctcgac ctgctcgcca ttttcctgag
    26581 ttgccagcca ggccatgcgc acttcatagg caataacctg aaccgccatc gccaggttca
    26641 gcgaactgta ttccgggtta gccgcaatcg cgacatgata atggcatttc tgcagctctt
    26701 cattggtcaa gccgacgcgc tcgcgaccaa acaccagcgc caccggggta tttgccgctt
    26761 cagcgacgct tttcagaccg cattcgcgcg ggtcgagcat cggccacggc agcgtgcggg
    26821 agcgagcgct ggtgcccacc accagactac aaccggctaa cgcttcgtcg agcgtatcga
    26881 cgatgtgagc attaccaatc acatcgctgg cccctgctgc cagggcaatc gcctgggagt
    26941 caggtttcac tagtggatta accagccaca gattggttaa tcccattgtt ttcatggcac
    27001 gggcaacaga acccatattg ccggtgtgtg acgtctccac cagcacaatt cgaatatttt
    27061 gcagcattgt ctttcttcat ctaaagatta ttcacgcatc ttatcataaa acgaagacag
    27121 atgccgatct cgctgctata ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga
    27181 gaccgatgca tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa
    27241 ttgccaaaaa ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc agtaacgatt
    27301 tcgtgaccaa cgtagataaa gctgccgaag cggtgattat cgacacgatt cgtaaatctt
    27361 acccacagca caccatcatc accgaagaaa gcggtgaact tgaaggtact gatcaggatg
    27421 ttcaatgggt tatcgatcca ctggatggca ctaccaactt tatcaaacgt ctgccgcact
    27481 tcgcggtatc tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg gtatacgatc
    27541 ctatgcgtaa cgaactgttc accgccactc gcggtcaggg cgcacagctg aacggctacc
    27601 gtctgcgcgg cagcaccgct cgcgatctcg acggtactat tctggcgacc ggcttcccgt
    27661 tcaaagcaaa acagtacgcc actacctaca tcaacatcgt cggcaaactg ttcaacgaat
    27721 gtgcagactt ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc gctgcgggtc
    27781 gtgttgacgg tttctttgaa atcggtctgc gcccgtggga tttcgcggca ggcgagctgc
    27841 tggttcgtga agcaggcggc atcgtcagcg acttcaccgg tggtcataac tatatgctga
    27901 ccggtaacat cgttgctggt aacccgcgcg ttgttaaagc catgctggcg aacatgcgtg
    27961 acgagttaag cgatgctctg aagcgttaat gactcaggcg ggtgatatca ctcacccgcc
    28021 ctcgcctttc aggcgctatt ccgaaatact tcctcaccgc tttactttct ttcgtcactc
    28081 tcccaccatc tttccccgat taatggatga taaagaagtc gcaaggaaag gttatggcgc
    28141 tgccagtgaa caaacgcgtt ctcaaaattc tgtttattct ctttgttgtt gccttctgcg
    28201 tttatttagt gccgcgcgtc gccatcaact tcttctatta tcctgacgat aaaatatacg
    28261 gtcccgaccc ctggtcggcg gaatccgtcg aatttacggc taaggacggt actcgtctgc
    28321 aaggctggtt tattccttct tcgacgggcc ctgctgacaa cgccatcgca accatcattc
    28381 atgctcacgg caatgccgga aatatgtccg cccactggcc gctggtcagt tggttacccg
    28441 agcgtaattt caacgttttt atgtttgatt atcgcgggtt tggtaaatca aaaggcacgc
    28501 cgtcccaagc cggattgctg gacgatacgc aaagtgccat caatgtggtg cgccatcgca
    28561 gtgatgtaaa cccacaacgg ctggtgctgt tcgggcagag tattggcggg gcgaatattc
    28621 tggatgttat tggtcagggt gatcgtgaag gcatacgtgc agtgatcctc gactccacct
    28681 ttgcctctta tgcgaccatt gccaaccaga tgatccccgg cagtggctac ttacttgatg
    28741 agagttacag cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt ctacttacac
    28801 atggtaaagc tgatcacgtt atcccatggc agcacagcga aaagttgtat agcctgacaa
    28861 aagagccaaa acggttgatc ctaatcccgg atggcgaaca cattgatgct ttttccgatc
    28921 gtcacggcga tgtttatcgc gaacagatgg tggattttat ccttagcgcg ttgaatccgc
    28981 agaactaacc catgatcgct agcacgataa tcattcacaa aactacctta agacatgcta
    29041 atccactggc cagaacagtt taagatgaga aaaattctgt gacgcttgcc aacatttctg
    29101 atgattagca ttcccttcgc catttccctg agcaaacttt agctattctt atcaattatg
    29161 cttatgggag atctacagat gatgcctacg cttgctccac catctgtcct ttcggctccc
    29221 cagcgccgct gccagatctt gctgacgctc tttcagccgg ggttgaccgc caacacggca
    29281 actttcagcg agcttaatgg tgtggatgat gatattgcca gtcttgatat cagcgaaaca
    29341 ggacgggaga tcctgcgcta tcatcaactc acactgacta ctggttatga cggcagctac
    29401 cgggttgaag gtacagtgct taaccaacgt ttgtgtttat ttcactggct acgacgtggt
    29461 ttccgtctgt gtccgtcatt tattaccagc cgattcaccc ccgccctgaa gagtgaactg
    29521 aagcggcgcg gaattgcgcg taacttttac gacgatacca atctacaagc gttagtgaat
    29581 ctctgctccc gacggctgca aaaacgcttt gaaacgcgcg atattcattt cctgtgtctg
    29641 tatctgcaat attgtttgct gcaacaccac gctggaatta cgcctcagtt taatccgctc
    29701 caacgtcgct gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat tggacgccac
    29761 tggcagcgtc gggcgctcca gcctgtaccg cctgatgagc cactgtttat ggcattactt
    29821 ttttccatgt tgcgggttcc cgatccactg cgggatgcgc atcagcggga cagacaactg
    29881 cgtcagtcta tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat
    29941 gatgaacagg ggttatgcga tcagctttat acccacctcg cccaggcgtt aaatcgcagt
    30001 ttgtttgcca tcggtattga taacaccctg ccggaagagt tcgcccgcct gtatccacgc
    30061 ctggtgcgca ccacccgcgc ggcgctggcc ggatttgaaa gtgaatacgg cgtccatctt
    30121 tctgatgagg aaagcggtct ggtagcggtg atttttggtg cctggctaat gcaggaaaac
    30181 gacctgcatg agaaacagat tattctactg accgggaatg atagcgagcg agaagcgcag
    30241 attgaacagc agctacgcga actaacgtta ctgccgctca acattaaaca tatgtcggta
    30301 aaggcatttt tgcagacagg cgctccgcgc ggcgcgacac tgattattgc gccttatacc
    30361 atgccgttac cgctcttttc accaccgctg atctatacgg acctgacgtt gacaacacat
    30421 caacaggagc agatccgcaa aatgctcgaa tcagcatgag ggaacaactt tcgggcgcaa
    30481 aaacattgcc ggaagcgcca ccagcgccat cacccagaac acgccgtggc cgagatattg
    30541 atacaggaaa ccggcgaaaa cggtcatgat ggcgatactg ccgcccatcg cgacggcaga
    30601 gtaaaccgcc tgtaaacgga tgacttcgct accctggcga gcagcaatat agcgcatggc
    30661 agccaggtgg cagaccgtga aggtaccgca atgcagaatt tgcaccacta tcaaccacgg
    30721 caacgccgta gttgctccca taatgcccca gcgcactacg ccgcaaatcg ccgagatcaa
    30781 cagcatatcg cgtgcactac aacggcggaa aagtttatta ctcagcgcaa agataatgac
    30841 ttccgccacc acgcccagcg accacaaata ccccaccgcc gaggccgagt agccagctgc
    30901 ctgccagtaa atggcgctaa aaccgtaata ggccgcatgt gccccctgca ataaacaaac
    30961 gcaggccaga aagcgccagt tctggcgaac caacgccaac cacgcagacc agccggtgct
    31021 ctcctgctgg cggcttgccc cttgtggctg aatcgtcgga cggatgagaa agccgagcag
    31081 catggatgcc acgcccaacg tcaacagcgc gaggatcacc cgataatcaa acatactgac
    31141 cagtttgccc gtcagcgccg agccaatgac aaacgccacc gagccccaca gtctcacttt
    31201 gccgtaatca agcgggaact gcttttgcca cgtattcgcc agtgcatcgg tcaacggtac
    31261 cagcggtgag aaaaagaggt taaagccaat catcaccagc atcagccacg ctacgtgcgc
    31321 ccccgcccag aaggcgacag caaagagaag tgtcagcagt gccagcacgc gcaaggcgga
    31381 aatcaggcgg gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga ggaaacgggc
    31441 aaccagaccc gcccccaata acaggccgat ggtttctggc gttaaaccaa tccctttaag
    31501 ccagacgctc cagaaaggta gaaaaatgcc gtaactaaaa aagtatgtga aatagccgag
    31561 cgccaaccag cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg cgttagtctg
    31621 aggttaatcg aatgttggtg caagttgaaa aataagaata ttcgagcctg cttcctgacg
    31681 gttatcctgc atatcaggaa atgacaaaaa agtgcggccc acaacacaac atgtaaaaaa
    31741 gaacgatgaa acaggtgaaa atcgcaattt attgaactga caaactcatg tgagacggat
    31801 aaggcgtgca tgtcgcatcc ggcaggaaac aataagcaca ttgtcagcca tctgcgttat
    31861 gccgttatac cagaaacgaa tatctcaacg cctgggcgtg gcgaggtacc agagtgtgta
    31921 ttacgtttaa atcacatcat cttgcaaagg gattggttat gaactcacta cgttattttg
    31981 attttggcac tgcccgcccc gttttattat taattgcccg tatcgccgtg gtcttaattt
    32041 tcattatttt tggttttccc aaaatgatgg gctttgacgg tacggtccaa tatatggcct
    32101 cgctgggcgc gccaatgccg atgctggcag cgattattgc ggtagttatg gaagtgcccg
    32161 ccgcgatatt aatcatactt ggctttttca cccgtccgct ggcggtgctg tttattttct
    32221 acacgctggg aactgcggtg attggtcatc attactggga tatgactggc gatgcggttg
    32281 ggccaaatat gattaatttc tggaagaata tcagcattgc tggcgcattc ctgctgctgg
    32341 caattaccgg gccgggggca atttctctcg atcggcgtta ggaaagatgc cggatgcggc
    32401 gtaaacgcct tatccggcct accgctccaa caccgattgt aggcctgata agacgcagca
    32461 agcgtcgcat caggcatcgt gcaccgagtg ctacaaaatc gaaattaccc cgtaggcctg
    32521 tgcatccggc attttataaa ttacagcaac gcaacccgca acgacatctc tgaaattgtt
    32581 gattcccctg gtgcaagcgc aatgaggtca ccaccttccg gacgatgatg gtcatccggc
    32641 gcatgactca tcggttccag acagaagaaa tcaaacgcat atcctttatc aaacgcaggg
    32701 tctgaaacaa agatgaaata acacggtgca ggtggcgtcg tttccatgat gatggcataa
    32761 ccctcctgcg gctgctcgat gcgggcctga ccattccatc cggcaaaacc attgtttacc
    32821 cactggcgcg gcaacggcgt gagctggtta aaatccagtt cctgcggtag ctgctcgcaa
    32881 aactcacccg ccagccactg ctcccgctcc agccagtaac cgctcgcctg tgcctgaacc
    32941 cgcgtttgcg gcgacaacgg aaaataagga tgccagccgg taccaaatgg cagcgtctct
    33001 gccccttgat tggtgacagc gagcgtcacc gtcagcgtat ccgccgttaa atgaaatgcc
    33061 tggcttaccc gatagtgata aacaccgctg agatgttcat acaccagaca caagctatca
    33121 tcgctgcgcg agacacattg ccactcgccc aaccagccat cgccgtgtag atagtgcgca
    33181 tcccactcaa cattcggttg cagttgatac tcacgcccct gccagacaaa acggttgccg
    33241 ctcacccggt ttgcaaatgg taccagcgga aaacaagagg catcggttgc cacaccgctt
    33301 tttttaccag gacgtagcag cggcgtcgta tcgcgccaga agccttcgat tacgccgccc
    33361 tgatcagaaa cgtccagctt tagcggcccg tgagataggg tatagatggt catccgcaga
    33421 ctccttaatc cgggaaatta atcacaactt tcccgcattt gccgctcgcc attagcgcgt
    33481 aagcatctcc cgcctgctcc agtgagaagc gatgggtaat agcgttacgc ggccacagtt
    33541 tccagtcagt caaatcgtgg gcgcattttt ccatatggaa caagctggtc acccaggagc
    33601 caataattct gcgttggtga tgcatcaggt cagcgctgac ctcaaattcc acttttccgg
    33661 tttcgccaat gtaaaccact cgcccccagt cggcggtaga ttgcaatgcc agcaagcgcc
    33721 ccgcagcatt accggaacaa tcaagcgcaa catccgcacc gccatgagtg agttcagcga
    33781 tgatctgcgg cagaccttcg gtggtcgcta aatagccgtg atccatcacc cctagctgtt
    33841 tcgccatcgc cagacgttcc ggcaacatat caacaccgat gatccgtttt gcaccgcgac
    33901 ctttcgccag catcatcgcc atcatgccga ccggccccag accaaccacc accacgttat
    33961 cgctaccgga aacctcgccg cgcaaaatcc cttcatacgc cgtaccaaca ccgcaactaa
    34021 taaacgcgcc atcttcgtaa ctcagcgcgt ctggcaggag gatcagatct ttttcttccg
    34081 ccagtaggta ttcggcatga ccgccgtcac gctgccagcc gtaagcggct tttccttcac
    34141 cggtgcagga gataggaaaa ccgcgacggc agttcgggca aaagccacaa ccggaaatgt
    34201 gatacaccag cacgcggtcg ccctctttaa aatggcgaca gccttgcccc atcgccacaa
    34261 tctgcccgca cggttcatga ccgttgataa atccctggta taacggttta tcgggtgctg
    34321 ccgccgtagc gcggtgctgg tggtagatat agtggacatc gcttccgcaa atcccggagg
    34381 atttcatttt gatcagtacc tggttaatcc ccggcgtcgg caccgcaact tcccgcagat
    34441 cgacggtcga atttcctggt aaataagctg ccagcatcgt tttcataaat ccctcattaa
    34501 caatacgatt aattttcatc cctgccgcac ccgcgccagg ggcgttaagg tttagcgttt
    34561 cgctttactg ctacgctggg tcagcaagat attcgccagc accgccacca cgatgatgac
    34621 gccgcgcacc acctgctgga aaaaggagtt aatcccgagc agcaccagac cgttaccgat
    34681 tagcgtaatc accagtacgc caagcagcgt accgaacaac gagccgcgac cgccggaaag
    34741 cgccgtaccg ccgaccacga ccgcggcgat gacgtcaaac tccagaccgt ttgcggcacc
    34801 tgcgttaccg gaaccgaggc gggccgccaa caaaatgccg gtcaccgccg ctaataatcc
    34861 cgaaagggta aaaataagaa tgcgtacccg acgaacgtta ataccgcaca actgcgctgc
    34921 agtggcatta ccgccaacag caaaaaccga gcgcccgaag gcagttttgc ggctaatgaa
    34981 cacaaacagc gcaaataaca ccatcatgat cagcgcggat accggcaccc cgagaaattg
    35041 tccgcccagc cagtccagca cttcgttttc gttaattggc actggcaagg cgttcgtcat
    35101 aaacagcccc attccgcgca gggcgctcca taaacccagc gtggcaacaa aacttggcac
    35161 gttaaacacg ccgcgcagca ccccggcgag cgttcccatc agcgcaccta acagcaacac
    35221 cagcagacaa gcaatcgcca gcggaacgtc aaattgcagc aaaaacgcca ggcacactga
    35281 aacaaaagcc accatcggcc caacgctgac atcaatttca ccggagataa taatcagcgt
    35341 catcgcccag gcggcgatcc caatggtggc ggcatcgcgc agcacgttca tctggttatt
    35401 caatgagata aagccaggcg cattcaggga gaagaccaga taaagaatgg caatcaccac
    35461 cagcaaaccg atctcattaa tatggcgact gacaaattgt ttgagcgaga cgctcttgcc
    35521 gtgcggcaat ggtaatgacg aagcagacat cttcaggttc ctcggtaaaa ttcatcagtg
    35581 cacagacaga atggcggaca tcagctcatc cacattgacc ggagagtgaa actcctgcga
    35641 gaacgtgccg tgctgtaata acaggatgcg gtcacacacc agcggcaact cctccacttc
    35701 actggagata aacaccacgc tttttccttc ggcagccagc tcgcggacaa tacggtaaat
    35761 ctgctgtttg gcttcgatat cgacgccgcg cgttggctcg tcgagcaaca aaatctggct
    35821 ggcggcatag acccaacgac cgatcaccac tttttgctga ttgccgccag aaagcgtgcc
    35881 gatgggcgtt tcgctactgg cggccttgac cgtcatccgc tgcatcacct cttcggtcag
    35941 gcggcggatg gtggaccatt gcagcacacc gttggcgctg attttttgcc gattggtcag
    36001 cactgtattt tcgtcaacgc ccaaccaggg aatgatcccc gcttctttgc ggttttctgg
    36061 cgtatagcca atgccgcgtt tcagcatgtc gccgtaatcg gggcgcgtga ttttctcgcc
    36121 gttgataaca atttcgccct gttcatacgt ctccagccca acaatcgcct tcagcaattc
    36181 actgcgccct gcccccagca gaccagcaat gccgagcact tcgccacgac gtagcgtaaa
    36241 actgatatcc tccagcttgg gcttatggcg taacgcacgg acttccagca cggcctgatc
    36301 cataatttcc tgaggggcaa ccggcgcaat atcaacgtga tcgcgcccga gcattagcga
    36361 cacaatatga tgcgtagagg tgttttcgag catcacatcg cccgccacct gaccatcgcg
    36421 cataacggtg gcacaggagg caatgcggcg aatttcttcc atccggtggc tgacataaat
    36481 caccgccacg cccagtgccg acatcttttt caccgcgctg atcaccagtt caacttccgc
    36541 actggcaagc gagctggtgg gttcatcaag aatgaccacg cgcggctcgc ccttcatcac
    36601 ccgcgcaatt tccaccagtt gcttttgcgc cgggcttagt gttgaaacaa gttgttcagg
    36661 actaacgtca acgcccaacg cctgcaagca acgttgggcg tcctgcgcca tttgcaggta
    36721 atcaatcatg ccgttgcggc ggggccactg accgaggcag aggttttccg ccactgtcag
    36781 cccttccacc agacttaact cctgataaac cgcacgaacc cccagttcag cggcacggcg
    36841 agtcagcgta gcttcgtcac cttccagtcg cgtctcgcca atccagatat caccgctatc
    36901 cggacgttcg ctaccggtca gcattcgaat gagggtcgat tttcccgcac cgtttttgcc
    36961 taacagcgcg cgaacttcgc ctttattgag cgtgaagtta acgttatcca gcgcaacgac
    37021 gccgggataa cgcttatttc ctgccaccac ttttgctacc gggactgcct ctgttgccgt
    37081 gaacataggg acctctgcga atcagcgatt agggcagacc atcaacgtgc gttgccagcc
    37141 actgtttacc gtcttccgtt ttggtataga gatcgattgg cacctgaatc actttttcac
    37201 cgtcggcttg tttattgata accttcaatg tttgcgtaaa aacagcattg cccattttct
    37261 taccggaaat atccaccacc gctttcagca cctggttgtt ttccagctcc tgagcaattt
    37321 cagtggtcat atcagaaccg aaaacagcaa tttttccggc ctgattttga ttacgtaccg
    37381 cttttaccgc gccgagtgtc gctccgcccg attcccccat aatagcgttg agatccggcg
    37441 tggagataat cagtttttca ccgacagaaa tcgctttatc taaaacagtc ccttcctgat
    37501 tagcgacaat ttgcgcgccg ggaacgcggg tttttaatac ttcttcaaac cctttacgac
    37561 gttgcacaca aacttcaaag gcttcgcaat tgatgacggc aattttcggc tggtcaattt
    37621 tgttggcaat aaaataatcg gcggctgcgt tacccagttt tttaccaaat tccagcgggt
    37681 caccaaccag atatgccgag acatatttat cgacaccctt ttgattaata caggtgttgt
    37741 agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta ctgccatttt
    37801 cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta tcaacaaagg
    37861 tactttcttt cgaaatatcg ccctgagcgt tagtttcaat taattgtacc tgtactgaag
    37921 aatcttttgc cgcatcctga acgccctgac gcactccggc gtaatatccc tgggtatcaa
    37981 ggtatattgc gccaatagtc atttctttat cagaagccct ggcaaatagt gcgctaccca
    38041 atagcgtagc cattaataat aaattacggg ttcttgtcat ttttttaggc atagtgcctc
    38101 ctgtagggtt tttatttaca acggcttatt ttaattattt gagcgtgagc ggcagcgcgt
    38161 gccgctcaaa tattacagtt aatagggttt agtgaacgta gctaaaaata aaaggcatca
    38221 cggtggtgaa taatgccgct ttcggccagg ctggattaat cgccatcaat tcagcgcgag
    38281 cagattcata gtgcgcaacc tcccctaatc ccgcggccgc cagcatcgcc accatcaggc
    38341 atttttcttt atgctgctgt tgtaaatcgc tatacagcga caacaagtca ggctgcgaga
    38401 cggcaaagaa atccgcttcg atactggttt tcgccatctc tttcgcccac tgtttcattt
    38461 cactaaacag ttgctgtgcg gtgtgttgtt cgcccagcag tcgcagcgcc attccttgcc
    38521 agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg gtgcgatcgc
    38581 cggtcgccgc cagacgtaaa caacgcgtcg cttcagtttc atcgccctgc gcgttggcgc
    38641 atactgcctg ccagaaccag atgtcgttgt cagtttgccc cggtaaacgg ccttcgctta
    38701 aattctccgg ataatgcagc gcggcatgaa gcagttcgct ggcctgctgc ggctctctgt
    38761 catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactggctg gtgaccttcc
    38821 cttccccacc ttcccacggg tggaatttgc gcgtggcgag aatgtccgcc gctttgtctg
    38881 cctgaccagt gagatgccac aaattgagca gttctgcggt catgtcgtcg cgtttcagcg
    38941 caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtgaca ccgcttaact
    39001 tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc tgataagcat
    39061 tatcaagata acgcgcggcc agctcataat cgtgctgctt attccacgca tggatcgcca
    39121 acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgctgc cataaggcaa
    39181 tggctttgcc gtagctacgc ttgttgtagt agaagcaggc cagtaaatgc cgggcaaacc
    39241 agcactcttc gatactctcc agcgccgcca cttcttccag cgtattcggg aaacggacaa
    39301 actgcgggaa ggcatcaata gcttttgcga ccagttctcc gcgttcagct tttggtagca
    39361 aactggcttg caggtaaagc ggtagcgtgc gctgacagtc cagtgcgttc agcatctctg
    39421 ccgccagggc gggcatgccc cagttaatca gttgcccggc ggtcatcagg gcgttaacgt
    39481 cgcgtccctg acaaagacct cgccactgcg cgagggctga ttcgctacga ccatcgaacc
    39541 agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc tcgcgctgca
    39601 aacgcgcgtt gtcctgacga ccacttaaca ccagtagcag gttatgcagg caaagcactt
    39661 cctgattggt tgggcaggcg cgaagacttt gttggcaaaa atccagacca gtgtcgaagt
    39721 taccattacg cgccgccagt cgtgccagac cataatagcc gcccgcttta ctgttgccgc
    39781 tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgcccctga cgttcgtaag
    39841 cactggcgcg aatcaaactc gcctgtccgc actgcggatt tttgttcagc gcatgtgcgc
    39901 gtttcagtgc ctgactggca tataccaccg cttgcgggaa atctgcacgg ttatattcca
    39961 gcattgccag cgccagatta cagcgataat ccagcggatc cagcgccacg ccgcgcagat
    40021 agtaatcgaa cggtgaacgg ctcgcgtgat gatattgctc cagatgttga ccgataaacc
    40081 aggtttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg acgtccggca
    40141 acggcaacgc ttgcgactga tgttcatgat aactcagtac aatattgccg tcggcatcgg
    40201 agagctcaat ggtcagcctt tcaggattga taccgtgcaa cacgccctgg atggcggtcg
    40261 ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt tcgcggatcg
    40321 ccaggcggta tccgttcaac ggagagatgg catacagccc ccactcaatc ccccgctcac
    40381 tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc agagaatgat
    40441 aaggcaggaa atactgctcg aaacgcttct cctcgtatgc atcaagccag gtaaaatcag
    40501 gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta ttgtcagtca
    40561 ggctcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt tttttacctg
    40621 gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga caccacgcgc
    40681 caacaaaatc gtactgtgat ttttcagcca tatatgaggt tggaacgggc acatttttat
    40741 agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg gtggcgatgg
    40801 ggaaagcgga gacggcccgt ttaccgtgat caaacaccgc agtcacatcc ggtgggaaaa
    40861 cgctctgatg cccttcaccc cctttcactg ccgggttggc ccaccacaag aaatgacgcg
    40921 gcgtggcgtt gccgttatag acgcgactgg cgatttccag cgccgcccgg tcagggcgca
    40981 gggtgaaacc tgtcatcacc tgtaaaccgt gcatcggctc cgtttcgccg acccacaccg
    41041 tctgtgcgcc gtcgtcatgg gcttcgaggg tgaaatcaac gggcataaag gtggtcgggc
    41101 gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggcccc agcagtccca
    41161 ccagcgccgg tttaatgact tcattgtgat aaacaaaatc gcgctgtttc actttatccc
    41221 acgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg tagtcgtttt
    41281 ccagccacac cgcctgccag gatttcaggg ttttctgctc gctcagcgta tcggtcacgc
    41341 cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg aacatgggat
    41401 gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc cacactttta
    41461 ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat ttgtggcagt
    41521 gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta aacaaggttt
    41581 agtaaagatg agagcctgca ttaacaatca acagattcgc caccataaca aatgcgtgat
    41641 tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc ggctggcgca
    41701 aatttcgatt ccggcggtca gtaatatttt gcaggaactg gaaagcgaaa aacgggtggt
    41761 gaatatcgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc tgattgcgcc
    41821 ggaaggagac tggacgctgt gcctgaacgt gacgcccacc agcattgagt gtcaggtcgc
    41881 taatgcctgt ttaagtccga aaggcgaatt tgagtattta cagattgatg caccgacgcc
    41941 gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat tgtggccgga
    42001 ccgcaccatc aatctggcgc tggcaatcca cggtcaggtt gatccagtga ccggcgtgtc
    42061 gcaaaccatg ccgcaagcgc cgtgggcaac accgattgag gtgaagtacc tgctggaaga
    42121 gaagctgggt attcgggtaa tggtcgataa tgactgcgtg atgctggcgc tggcggagaa
    42181 atggcaaaat aattcgcaag tgcgtgattt ctgcgtaatc aacgttgatt acggcattgg
    42241 ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggca gtggacagat
    42301 tggccacacc atcgttaatc cggatggcgt cgtctgcgac tgcgggcgtt atggctgcct
    42361 ggaaaccgtc gcctcgttaa gcgcattaaa aaaacaggcg cgggtatggc taaaatcaca
    42421 accggttaat actcagcttg atcctgaaaa attgactacc gcgcagttaa tcgctgcctg
    42481 gcagagtgga gaaccgtgga tcaccagttg ggttgaccac agtgccaacg ccattggttt
    42541 gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt acggtcgcag
    42601 ttgtgccttt ggtgagaact ggcttaatac cattattcgc cagacgggat ttaacccgtt
    42661 cgaccgcgac gaaggaccga gcgtgaaagc gacacagatt ggctttgggc aattaagccg
    42721 cgcacaacag gtactgggaa ttggctattt gtatgttgag gcgcagttac ggcagatttg
    42781 atggcgcgat aacgtagaaa ggcttcccga aggaagcctt ttttcaacag attaaccgtt
    42841 tcgcttatgc gtaaaccggg taacgtgcgc agatgtcgag aactttacct ttgatgcgct
    42901 cgataacggc ttcatcattg atgctgtcca gcacgtcaca catccagcca gccagttctt
    42961 tcgcttcggc ttctttaaag ccacgacggg taatcgccgg agtacctaca cgaataccgg
    43021 aagtcacaaa cgggctcttc ggatcgttcg gtacgctgtt tttgttcacg gtgatgttag
    43081 cacggcccag agcggcgtct gcttctttac cggtcaggtt tttatcaacc agatcaacca
    43141 ggaacaggtg gttatcagta ccaccagaaa ccactttgta gccgcgttcg aggaacactt
    43201 ctaccatcgc tttggcgttt ttcgcgacct gctgctggta agttttgaac tcaggttcca
    43261 ttgcttcttt cagagcaacc gctttaccgg cgattacgtg catcaacgga ccgccctgac
    43321 caccagggaa aacggcagag ttcagttttt tgtacagctc ttcgctacca cctttcgcca
    43381 ggatcaggcc gccgcgcgga cccgccaggg ttttgtgagt ggtggtagta acaacgtgag
    43441 catgaggaac cgggttaggg tagacaccag cggcaaccag gcccgcaacg tgcgccatat
    43501 caacgaacag gtaagcaccg atgctgtcag cgatttcacg cattttcgcc cagtcaacca
    43561 cgccggaata tgcagagaag ccaccgataa tcattttcgg tttgtgttct ttggcttgtt
    43621 tttccagatc ggcgtagtcg atatgaccgg tagcatcgat accgtaagga acgatgttgt
    43681 acagtttacc ggagaagtta accggagaac cgtgagtcag gtgaccgcca tgcgccagat
    43741 tcatacccag aacggtatcg cctggttcca gcaacgcggt gtagaccgca aagttagcct
    43801 gggagccaga gtgcggctgg acgttagcgt agtcagcgcc gaacagttct ttcgcacgat
    43861 cgatcgccag ttgttcaacg atatcaacat actcgcaacc gccgtagtag cgtttgcccg
    43921 gataaccttc agcatatttg ttggtcagct gagaaccctg cgcctgcatt acgcgcgggc
    43981 tggtgtagtt ttcggaggcg atcagttcga tgtgctcttc ctgacgtact ttttcctgct
    44041 ccatagcctg ccacagttcg gcatcataat cggcaatgtt catttcacgc tttaacatcc
    44101 gcatctcctg actcagctaa caataaaatt tttggccttt ataggcggtc ctgttggaca
    44161 acggcgaaca gtataaccga atcattgtgc gataacaggt cttgacaaag gaatttacgc
    44221 aaacgattac cttcaggcta cgcaaggctt tggagaataa agagcttgca accggaaacg
    44281 gatttctttt caggtttgtg atgcaaattt ttcacttcat cacattcttt ctgaaaaaca
    44341 ccaaagaacc atttacattg cagggctatt ttttataaga tgcatttgag atacatcaat
    44401 taagatgcaa aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct acagtaaaag
    44461 ccaccatccc tttactggtg gaaacgggac caaagttaac cgcccatttc tacgaccgta
    44521 tgtttactca taacccagaa ctcaaagaaa tttttaacat gagtaaccag cgtaatggcg
    44581 atcaacgtga agccctgttt aacgctattg ccgcctacgc cagcaatatt gaaaacctgc
    44641 ctgcgctgct gccagcggta gaaaaaatcg cgcagaagca caccagtttc cagatcaaac
    44701 cggaacagta caacatcgtc ggtgaacacc tgttggcaac gctggacgaa atgttcagcc
    44761 cggggcagga agtgctggac gcgtggggta aagcctatgg cgtcctggct aatgtattta
    44821 tcaatcgcga ggcggaaatc tataacgaaa acgccagcaa agccggtggt tgggaaggta
    44881 ctcgcgattt ccgcattgtg gctaaaacac cacgcagcgc acttatcacc agcttcgaac
    44941 tggagccggt cgacggtggc gcagtggcag aataccgtcc ggggcaatat ctcggcgtct
    45001 ggctgaagcc ggaaggtttc ccgcatcagg aaattcgtca gtactctttg actcgcaaac
    45061 cggatggcaa aggctatcgt attgcggtga aacgcgaaga gggtgggcag gtatccaact
    45121 ggctgcacaa tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg gcaggtgatt
    45181 tctttatggc tgtcgcagat gacacaccag tgacgttaat ctctgccggt gtcggtcaaa
    45241 cgccaatgct ggcaatgctc gacacgctgg caaaagcagg ccacaccgca caagtgaact
    45301 ggttccatgc ggcagaaaat ggcgatgttc acgcctttgc tgatgaagtt aaggaactgg
    45361 ggctgtcact gccgcgcttt accgcgcaca cctggtatcg tcagccgaat gaagccgatc
    45421 gcgctaaagg tcagtttgat agcgaaggtc tgatggattt gagcaaactg gaaggtgcgt
    45481 tcagcgatcc gacaatgcag ttctatctct gcggcccggt tggcttcatg cagtttgccg
    45541 cgaaacagtt agtggatctg ggcgtgaaac aggaaaacat tcattacgaa tgctttggcc
    45601 cgcataaggt gctgtgattt gatgttgccg gatggaaaca tccggcaacc cttgacccgt
    45661 tttaaatcgc cgcatcatcc tcttcaccgg tacggatacg aatgacccgt gccacgtcga
    45721 agacgaagat tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata atggtatcga
    45781 cacaggtatc gacaatgtcg tccggtacga caatctcaat tttcactttc ggcagaaaat
    45841 ccaccatata ctccgcaccg cggtacagct cggtatggcc tttctggcgg ccaaaacctt
    45901 tcacctcagt gaccgtcatg ccggtaatac cgacttcagc cagtgcttcg cggacatcgt
    45961 ccagcttgaa gggtttaata atcgcatcaa tctttttcat gctattcctt gaaaaggtcg
    46021 cctgtctttt gatctgctaa acgtaacaca taacgccaat tcattccttg aaatcgtttg
    46081 catccagttc gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc cccgccattc
    46141 tcgccgcgtg ggtgacgttg cctttggtga tttgcagcag cttacgcaaa tagttgagtt
    46201 caaactgatt acgcgcctca acaaaggttg gcagcgccgt gttttcgccc tccagcgcct
    46261 gctccaccag cgcatcacta attaccggag atgatgtcag cgccacgcac tgttcaatca
    46321 cgttgaccaa ctggcgcacg ttgcccggcc agctcgcagt catcaggcgt ttcattgcat
    46381 cggtggagaa cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc aacaggtgat
    46441 tagccagtag cggaatgtct tctgtgcgct ccgccagggc cggaattttc aggctgacaa
    46501 cgttgaggcg gtaatagagg tcttcacgga attccccgcg cgtcatcgct tttggcaggt
    46561 cgcggtgggt cgcagaaata atccgcacgt cgatatcaat atcgcggtta ctgcccagcg
    46621 ggcgcacttt acgctcctgc aacacgcgca gcagtttgac ctgcaacggt gcgggcatat
    46681 cgccaatctc atcgagaaat agcgtaccgc cttccgccgc ctggaataaa ccttcgcgat
    46741 tgctgacagc gccagtaaac gcgccacgtg catgaccaaa cagctccgac tccagcaatt
    46801 gctcgggtaa tgcgccacag ttaatagcaa taaatggctt gctgttgcgc gggctggcgt
    46861 tgtggatagc ctgggcgaaa atctctttcc cggtgccgct ctggccgtta atcaaaacgc
    46921 taacgtctga ttgtgccacc agccgcgcct gttccagcaa gcgcagcatc agcgggctgc
    46981 gggtgacaat cgcctcgcgc caacgttcgt cggtggctgg cgcggattgt tccagcgcat
    47041 cgtcaattgc ctgatatagc gcatctttat cgacaggctt ggtgagaaaa ctgaacacgc
    47101 cctgctgtgt tgcagcaacg gcatcaggaa tagaaccatg cgcggtaaga ataattactg
    47161 gcattcccgg ctgcactttc tggatttcag caaacagctg cataccgtcc atttcatcca
    47221 tccgcaggtc gctgatgact aaatctactt tttcgcgatt cagtacccgc aatccttcag
    47281 caccactttc cgccgtgacc acactgtagc cttcgctggt caggcgcagg ccaagcagtt
    47341 tcagcaatcc cggatcgtca tcgaccagta ataaatgcgc aggtttatgg ctcatcagga
    47401 gtgacctcat cggtggatgg cgcgggcttt tcactctcgt gcagcgtatc cggcgagaaa
    47461 tttccggccg gtttgcgggt tgagagctgg cgttcaatat cggtcaggtt ttccagcttg
    47521 cgggtagtga gttccagttg ctgttgtaga acgtgatgtt gctggcgcaa agtatccagc
    47581 tcgctgtcgc tggactgctg gagtttgctg tagcgttggc gctcttccgc cagttgcagt
    47641 tgcagcgcct gaccgtcgcg ccaaagttga tacagtgggc gaacctgtgc cgggatctcg
    47701 gtacttagcg catcaatgcg cgcgaccagc tggcggcgct catacggagt aattttggcg
    47761 tcggcgagca aaatcccttg tttaaaggta ttttgccagt tgccgtcgtc atattggcgg
    47821 gcttgctgac gcgactgcgc aggcattaaa cgatcagcac aatccatcgc ccgcagccag
    47881 taaagcggat tggtttcggt agatttacct tgcagcgccc agatgtcgct acattcagta
    47941 gaaagatagt cagccagttg ataaaccgga attttttctt ctgctggcgt atcaatggct
    48001 ggcctattgt gactctgcac gcaacccagc aatgccagac atggcagccc tgccagccac
    48061 agccgtcggg gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact taaagcctat
    48121 cccagtgggc gtaattgttg caggcagttt ggacatggac agcgcggaga aaccggagcg
    48181 tacatgtcgt acgtgaagat ttcgagcact gcccggggcc gaactgacaa ataaaatagc
    48241 ctgatgggat gggctgttag cattatttcg tgtttttcga cgacggtaat tcaatgcgga
    48301 aacaaacgtc ttgcccgctc tcgtcgacca gatacagttc cccttgcata cggcgaatac
    48361 aatccctggc aatgcttaat cccagaccgc tgcccttcac cgcccctttt cgctggtggc
    48421 ttccctgaaa aaagggttcg aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg
    48481 tattgatgac atcaatataa acccgcgcac catgtaaact gctgcgaagg caaatgttac
    48541 cggattcagc cccgtagtgc accgcattgg agtaaagatt atccagtacg ctcatcagca
    48601 gcattggctc cgccaggcaa gctgttgctt tgagatcaac gtcggtatgc atcattttag
    48661 cccgtgcggg caggctatga gcagaaacca ctgtctccac cagcggtgct aactcaacat
    48721 tctccagttc caccgcactg tccgcctgtt tacggttgta atcaagcagt tgttcgatca
    48781 gcttttgcaa attgcggctg ctgctatcaa gaatgctcac cacctctttt tgctctggcg
    48841 taagcggccc gacaacctga tcagccagta attcagtgcc ctcgcgcata ctcgccagtg
    48901 gcgtttttaa ttcatgagat aaatgtctta aaaattgatg gcgttgggat tccagccatg
    48961 acaggcgctc acttaaccaa agaatacgtt gcccaaccga gcgtaactcg ctcggtccac
    49021 tgaacgagac gctattgccc agagaacgcc cttcccccag ccggttgatc atgcgctcga
    49081 tatttttcac cggaccgata atcatcctcg tgaaaagcag taccatcacc agactcacca
    49141 gaaatagcac cagcgattgc caaccaaaat attgcccacg ttcggcgatt tcacgctgaa
    49201 gttgctgccc acgagagaac accactgtgc gcgtggcctg caccatttcg gtattggcac
    49261 tggcaaaggc ttccagacgc gcggcggcag cagcatcggg accgctgttg ttacactgaa
    49321 gttgagccag attgttcaaa tcctgacgta atgcctggta gagtttatcg tccggcagca
    49381 cgcctgcgtg ggcatcgagc atttcgctgt aacgcttgcg ctggctttga taaaccttcg
    49441 caagcgtagg gtcgtccagc acgcagtact gacggtagct gcgctccatc tccagtgccg
    49501 cgttggtcat cgcttcactg cgccgggcat caataagcgt agtgcggtta accagcgccg
    49561 cctgatcgct aagtgcattc aggctttgcc aggcttgcca tgccagcacc aacaggggca
    49621 gcagaatcag caaaaatgcc agcattacca gttgtcgtaa tgagcgggga aaaacgggcc
    49681 agcgtttcaa ggcgttactc tcgtcagacg cgaatagcct gatgctaacc gaggggaagt
    49741 tcagatacaa caaagccggg aattacccgg ctttgttatg gaataaggcg gtgcctaact
    49801 cgacgtttcg cccgatggtt gatatagcta cgctgatatc agaagttgga cggcaggcac
    49861 cttgttgtgc gtcattcgaa ttttatgtag cacgtcccga aggggctgac ataagtcggt
    49921 gaatgagcca ctgcttacta ttatgcagta actgtgccaa taaagaaaat agtttggtaa
    49981 catactgatt atacgctgct ttgaggggtt tatggcttct ccgctatttg aatgtaaatc
    50041 agccactgtg tcgctaaaaa gagacaactt aagataaact tattagataa tattaaaatc
    50101 aatgagttaa gtgtcaccaa aaagcgacac ggcaaaccac ccattgtcgt gatttacaga
    50161 cacaaaaaag ctcccgaagt tgggagctca taatagcggt tggtgcttaa tgccggatgc
    50221 ggcgtgaatg tctcatccgg gctgcaatac caatgggttg acgacttacc ccaactgctt
    50281 acgtgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt ccggatgcca
    50341 ggagttgctg acggtacgga aaactcgttc cgggtgcggc atcataatgg taactcgacc
    50401 actttcagtc gtgaccgccg taataccgtt cggtgaaccg ttcgggttag ccgggtaggt
    50461 ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt tgctttccag
    50521 tgccgccaga tgcgccccat cacgtacttc cacgcgccct tcaccatgag agacagcaat
    50581 cggcatctgc gagcccacca tcccctgcaa cagcagcgac gggctttggg ttacttctac
    50641 cagactgaaa cgcgcttcaa agcgatcgga ggtgttacgc acaaaacgtg gccacaactc
    50701 gctacccggg atcagttcac gcagattaga catcatctgg caaccgttac atacccccag
    50761 cgccagcgtt tgcggacgat ggaagaaggt tgcaaactca tcgcgtacac ggtcattgaa
    50821 caggatagac ttcgcccaac cttcaccagc gcccagcaca tcaccgtagg aaaaaccacc
    50881 gcacgctacc aggacgtgga aaccctccag gcccgtgcgt ccagccagca gatcgctcat
    50941 atgcacgtcg atagcatcga agcccgcacg gtggaaagct gccgccattt caacatggga
    51001 gttcacaccc tgctcacgca gtacggcaac tttcgggcgc gcgccagtgg caataaacgg
    51061 tgctgccaca tcttcgttga tatcaaacga cagtttcaca ttcaggcccg gatcggcgtc
    51121 gtttgatttc gcctggtgtt cctgatcggc acactccggg ttgtcacgca gacgctgcat
    51181 ctgccaggta gtttctgccc accagacacg caacgtggtg cggctttcgc taaatacagt
    51241 ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccaa cataatggac
    51301 gcaatcagca agcccatgct gtgccagtac cgcttcaacg gcttcacggt cagcggctcg
    51361 aacctgaatc accgcaccca gttcttcgct aaacaatgca gccaggcgat cgtcacccag
    51421 agtggcgata tccgcgtcaa tgccacaatg accagcaaac gccatttccg ccagcgtcac
    51481 cagcaggcca ccgtcagagc ggtcgtggta cgcgagcagc ttacgctgtg caaccagtgc
    51541 ctgaatcgcg tcatagaaac ctttcagttg ggcaacatcg cgtacatctg ccggtttgtc
    51601 gccaagctga cgataaacct gcgccagtgc cgttgcgccc agcgcgttat tgcctttgcc
    51661 caaatcaatc agcaacagtg cgttatcttc ggtagaaagc tgcggcgtaa tggtgtgacg
    51721 tacatcttcc acgcgggcaa atgcggaaat caccagcgac aacggcgacg tcatttcgcg
    51781 ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac ccaccgggat
    51841 cgtcaggccc agcgccggac aaagttcttc acccacggct ttaacggctt catacaggcc
    51901 tgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt taatgcgttt
    51961 gatatcgcca atttgtgttg cggcgatgtt ggttaacgct tcaccgaccg ccagacgggc
    52021 agaagcggcg aaatccagca gcgcaaccgg cgcacgttcg ccaatcgcca tcgcttcacc
    52081 gtagtagctg tcgaggctgg cggtagtgac tgcgcagtta gcgaccggca cctgccacgg
    52141 acccaccatc tgatcgcgcg ccaccatgcc ggttacgctg cggtcaccaa tggtcaccag
    52201 gaaagttttc tccgccacgg tcggcaggtg cagcacgcgt ttcaccgcgt cagcaatggt
    52261 aatcccttca cgaaccagcg catcaccctt cgctttcagc gtttgtactt cgcgggtcat
    52321 cttcggcgtt ttaccaagca gtacgtccag cggcagatcg atcggctgat tatcaaaatg
    52381 acgatcgtgc aaagaaagat gcagttcttc ggtcgcttca ccaatcaccg cgtatggcgc
    52441 gcgctcacgc ttacacagtt cgtcaaacag cggtaactga tcggcagcaa ccgccagcac
    52501 gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg gttcgtcgct
    52561 caggatatcg cgcagttcaa atttaccgcc gcgcccgccg tcgctcacca gttccggcat
    52621 ggcgttagaa agaccgcctg cgccaacgtc gtggataaac aggattgggt tggcatcacc
    52681 aagctgccag caacggtcga tcacttcctg gcagcgacgc tccatctccg ggttgtcgcg
    52741 ctgtacggaa gcaaagtcga ggtccgcatc agactgacca gatgccatgg aagacgccgc
    52801 gccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct ttgcaccaac
    52861 gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgattccac ccgccagcat
    52921 gatcggtttg tgataaccgc gcagctcttc gccattgtgg ctgttcactt tttcttcata
    52981 agtacggaag tagccgttca gtgccgggcg accaaattcg ttgttaaacg ccgcgccgcc
    53041 cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg cgctcaggct taccgaaatc
    53101 ttcttcccac ggctgttcaa agcccggaat acgcaagttg gatacggaga aaccgaccag
    53161 accagctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt caccgccgga
    53221 accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtggg tttcaacttt
    53281 catcagaata tgcgccggtt cctgatggaa atcgtagcgg cccgtttcgt ggtcagcaaa
    53341 gtagcgaccc acttcagaac cttccattac ggcggcgtta tctttataag cagagagaac
    53401 gtggtctggc gtgatttcga aagtattttt gatcatcttg aacagcgatt tcggctgctg
    53461 ttcaccgtcg ataatccagt cggcgttaaa aattttgtgg cggcagtgct cagagttcgc
    53521 ctgagcaaac atatacagtt cgatgtcgtt cgggttacga cccagctttg tgaacgcatc
    53581 ctgcagatag tcaatttcat cttccgccag cgccagacca agacgcaggt tagcgtcgat
    53641 tagcgcctga cggccctgcc ccagcaaatc aatgctggtg accggggttg gctgatgatg
    53701 agcaaacaag tgttctgcat catctaaagc aaaaaagacc gtttccatca tgcggtcgtg
    53761 cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt ctatatagta
    53821 agcaacgccg cgctcaaggc ggttaacctg ttgcagcccg cagttatggg caatatcggt
    53881 cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca agagtttgcc
    53941 ttgcggggcg tggctggcga gtgccgggcc atatttcagc aggcgttcaa gttgtgcgtg
    54001 ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg cgtaaatatt
    54061 gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc ggaatgccga
    54121 caatgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta agctttcggt
    54181 gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga cgaaaccgtt
    54241 tgcgtagaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg gctgtaataa
    54301 gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa cctttcacta
    54361 ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa ttgaaaaaat
    54421 taaagattaa ttatctgttc atcggtattc tggcactgct actcgcggtc gctctctggc
    54481 catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa gcgcggggag
    54541 agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac gggaaacctt
    54601 ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggtgta aaactgaaag
    54661 tgaccgtgcg gcagaatatc agccagctgt ttgacgacct cgataatggt aacgccgacc
    54721 tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag ccaggtccta
    54781 cctattattc cgtgtcacaa caactggttt ataaagtcgg tcagtatcgc ccacgtacgc
    54841 tgggcaacct gacggcggag caactcaccg ttgcaccggg tcacgtggtg gttaacgatc
    54901 tccagaccct gaaagacaca aaattcccgg aattaagctg gaaggtagac gacaaaaaag
    54961 gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc attgctgatt
    55021 ctgtcgccat cagcctgttt cagcgcgttc acccggaact cgccgtagcg ctcgatatca
    55081 ccgatgaaca accggtgacc tggtttagcc cgttagatgg cgataatacc ctttccgccg
    55141 ccctgctcga cttcttcaac gaaatgaatg aagacggtac actggcacgc attgaagaga
    55201 aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt ttacgcgccg
    55261 tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa gaaattgact
    55321 ggcgtttgct ggccgctatt gcttatcaag aatcgcactg ggatgctcag gccacttcac
    55381 cgacgggtgt gcgcggcatg atgatgttaa ccaaaaacac cgcgcaaagc ctcggcatta
    55441 cggatcgtac cgatgccgaa cagagcatca gcggcggcgt gcgttatttg caggatatga
    55501 tgagtaaagt gccggaaagt gtgccggaga acgagcggat ctggtttgcc ctcgccgcgt
    55561 acaatatggg ctatgcgcat atgctggatg cccgcgccct gacgacaaaa accaaaggta
    55621 atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag aaaccctatt
    55681 acagcaagct gacttacggc tacgctcgtg ggcatgaagc ctacgcttat gtcgaaaata
    55741 ttcgtaagta tcagattagc ctggtgggtt atctgcagga gaaagagaag caggctacag
    55801 aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag ctgggcaaag
    55861 agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg acccactctc
    55921 cctctctgct gttttccaga aaagggagtg aagagaaaca aaattaatct gtcgaggatt
    55981 gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca ctgagcaacg
    56041 ccgcgcactc atccgccagt attccttccg taatttccac ccggtgattc atacccggat
    56101 gatgtagcac atccattaaa gatcccgcag cgccagtttt cgcgtcacgc gcaccaaaga
    56161 ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacag ggttcaagcg
    56221 tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagaccg ccctgccgta
    56281 gggccatgat ttctgcatgt gcggtgggat catgtcgacc aatcggacgg ttccagcctt
    56341 cgccaataac acgattgtta tgcactaata ccgcgccgac cggtacttcc cgctcatccc
    56401 aggcacgctt cgccagcgtc attgcatgac gcatccagta ttcgtggcta aattcgactt
    56461 cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga cgctatgctt
    56521 tactctattc cagttgctgg agttcaccgc gcggagttac tcgccaacga tgctgacaga
    56581 aataaagcag cgggttgtcc tgattactgt cgctatagcc actgtacagc cgcagcggcg
    56641 tgccgatttt gcgctccagt tgcgcgacct tttcatgtcc cagacaacgc atcgtcaata
    56701 cccaaccacc atagccacgc tgaatctgac tggcgataag attaacccgc ggcagccagg
    56761 gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc aaccagatat
    56821 cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga aaggcggtaa
    56881 cattgtcacg aaaccagcgt acgaaatcgg cctgcaacgc ctgcaaacgc gcttcattgt
    56941 gaccaaaagt gcatccccac agaagcagac tcatcggcca gcgtgccgca cgacctttta
    57001 tcaataacgc aatggctatg atcggcaaca gcggcagaac aagtaacgca ttcagcggtt
    57061 ggcgacgcag taaataacgc aaaaaactgc cgaacatatc ctgctgatgc aatgttccat
    57121 ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc tctggactga
    57181 aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca cttttccggc
    57241 aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta gcggcaatgg
    57301 aatataaaat tcactcgcgt ttgtctcata ttgcctgttg tcgccactat ttcatcgcga
    57361 ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt gcccagagtg
    57421 ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat ttaagctctc
    57481 agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc gcgcaaaaac
    57541 tcggctataa aggttttccg gcacttaagt tagcgttgag tgaagcgctg gcaagccagc
    57601 cggaatctcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg ttacggctag
    57661 tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgtta aacgttaata
    57721 gtgaagagaa actgcatgaa tgcgtggcaa tgttgcgctc tgcgcggcgg ataattctga
    57781 ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg atgaagattg
    57841 gcttcaatgc tgccgcagtg cgcgatatgc atgcgctact cgctacagta caggcgtcgt
    57901 cccctgacga tctgttatta gccatttcct acaccggtgt gcgacgcgag ttaaacctgg
    57961 cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc tttactccca
    58021 atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa caagcgacaa
    58081 acagtgcttc aatctctgct tgtcacgcgc agggaatgtt aacggatttg ctgttcattg
    58141 cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcatagc gaagcgctgg
    58201 tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt gtgttgtttt
    58261 gagagtttcc ttatggcgct gttaatcact aaaaaatgca tcaattgtga tatgtgtgaa
    58321 cccgaatgcc cgaatgaggc gatttcaatg ggcgatcata tctacgagat taacagcgat
    58381 aagtgtaccg aatgcgtagg gcactacgag acacccacct gccagaaggt gtgcccgatc
    58441 cccaatacta ttgtgaaaga tccgcaacat gtagagacgg aagaacagtt gtgggataaa
    58501 tttgtgctga tgcaccacgc ggataaaatt taatattttg ctctggaagt agagtgttaa
    58561 ttatcttata gagaaacctt aacgctatta tatttatttg attgatgaca ttagcataat
    58621 tattcactaa gt
//
LOCUS       DADSZD010000009        56976 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.009, whole genome shotgun sequence.
ACCESSION   DADSZD010000009 DADSZD010000000
VERSION     DADSZD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.009"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            106..291
                     /locus_tag="J1Z28_000464"
     CDS             106..291
                     /locus_tag="J1Z28_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715330.1"
                     /translation="MSRRYSLATITLVILFCGRPHCRKISVRQYYQKRVIFYPTTLQR
                     TAFKKCLFLIRDIIISS"
     gene            252..371
                     /locus_tag="J1Z28_000465"
     CDS             252..371
                     /locus_tag="J1Z28_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBA7715331.1"
                     /translation="MPLSYTGYHHFIMMSLMSGEHNTFALSLQDEAPGLVPAD"
     gene            406..748
                     /locus_tag="J1Z28_000466"
                     /pseudo
     CDS             406..748
                     /locus_tag="J1Z28_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(1122..1388)
                     /gene="minE"
                     /locus_tag="J1Z28_000467"
     CDS             complement(1122..1388)
                     /gene="minE"
                     /locus_tag="J1Z28_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBA7715332.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(1392..2204)
                     /gene="minD"
                     /locus_tag="J1Z28_000468"
     CDS             complement(1392..2204)
                     /gene="minD"
                     /locus_tag="J1Z28_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBA7715333.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2228..2923)
                     /gene="minC"
                     /locus_tag="J1Z28_000469"
     CDS             complement(2228..2923)
                     /gene="minC"
                     /locus_tag="J1Z28_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBA7715334.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3443..3811
                     /locus_tag="J1Z28_000470"
     CDS             3443..3811
                     /locus_tag="J1Z28_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715335.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSVQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVN
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3914..4315)
                     /locus_tag="J1Z28_000471"
     CDS             complement(3914..4315)
                     /locus_tag="J1Z28_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HBA7715336.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4557..4850
                     /locus_tag="J1Z28_000472"
     CDS             4557..4850
                     /locus_tag="J1Z28_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715337.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4922..5581
                     /locus_tag="J1Z28_000473"
     CDS             4922..5581
                     /locus_tag="J1Z28_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBA7715338.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5658..6119
                     /locus_tag="J1Z28_000474"
     CDS             5658..6119
                     /locus_tag="J1Z28_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415699.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBA7715339.1"
                     /translation="MAEHLMSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDED
                     TDEIYFTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLA
                     EGKGLPAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(6326..6715)
                     /locus_tag="J1Z28_000475"
     CDS             complement(6326..6715)
                     /locus_tag="J1Z28_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin E"
                     /protein_id="HBA7715340.1"
                     /translation="MEAFAQKGILIKVLDDGIKKLCSIAAGVVEGKLIPELKNKLKSA
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKIINTCNEYQKRHGKKTLFEVSEV"
     gene            6912..7331
                     /gene="umuD"
                     /locus_tag="J1Z28_000476"
     CDS             6912..7331
                     /gene="umuD"
                     /locus_tag="J1Z28_000476"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBA7715341.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLTPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            7331..8599
                     /locus_tag="J1Z28_000477"
     CDS             7331..8599
                     /locus_tag="J1Z28_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBA7715342.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRAMVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(8644..9174)
                     /gene="dsbB"
                     /locus_tag="J1Z28_000478"
     CDS             complement(8644..9174)
                     /gene="dsbB"
                     /locus_tag="J1Z28_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBA7715343.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(9320..10861)
                     /gene="nhaB"
                     /locus_tag="J1Z28_000479"
     CDS             complement(9320..10861)
                     /gene="nhaB"
                     /locus_tag="J1Z28_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBA7715344.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRNQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            11083..11802
                     /gene="fadR"
                     /locus_tag="J1Z28_000480"
     CDS             11083..11802
                     /gene="fadR"
                     /locus_tag="J1Z28_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBA7715345.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(11853..13385)
                     /locus_tag="J1Z28_000481"
     CDS             complement(11853..13385)
                     /locus_tag="J1Z28_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBA7715346.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            13715..15013
                     /locus_tag="J1Z28_000482"
     CDS             13715..15013
                     /locus_tag="J1Z28_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBA7715347.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            15023..16093
                     /gene="dadX"
                     /locus_tag="J1Z28_000483"
     CDS             15023..16093
                     /gene="dadX"
                     /locus_tag="J1Z28_000483"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBA7715348.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPRARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVLTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(16150..17886)
                     /locus_tag="J1Z28_000484"
     CDS             complement(16150..17886)
                     /locus_tag="J1Z28_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBA7715349.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(17981..18895)
                     /gene="ldcA"
                     /locus_tag="J1Z28_000485"
     CDS             complement(17981..18895)
                     /gene="ldcA"
                     /locus_tag="J1Z28_000485"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBA7715350.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            18995..19606
                     /gene="emtA"
                     /locus_tag="J1Z28_000486"
     CDS             18995..19606
                     /gene="emtA"
                     /locus_tag="J1Z28_000486"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBA7715351.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(19668..20342)
                     /locus_tag="J1Z28_000487"
     CDS             complement(19668..20342)
                     /locus_tag="J1Z28_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBA7715352.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQL"
     gene            20543..20797
                     /locus_tag="J1Z28_000488"
     CDS             20543..20797
                     /locus_tag="J1Z28_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA7715353.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(20847..22817)
                     /locus_tag="J1Z28_000489"
     CDS             complement(20847..22817)
                     /locus_tag="J1Z28_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7715354.1"
                     /translation="MRLKKRYLCTVLSLAFTQQSVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQISGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(22843..23697)
                     /locus_tag="J1Z28_000490"
     CDS             complement(22843..23697)
                     /locus_tag="J1Z28_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBA7715355.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFHRQGG
                     CVSGISVACKMLTTLGLTIDNAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(23694..24506)
                     /locus_tag="J1Z28_000491"
     CDS             complement(23694..24506)
                     /locus_tag="J1Z28_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA7715356.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            complement(24516..25274)
                     /locus_tag="J1Z28_000492"
     CDS             complement(24516..25274)
                     /locus_tag="J1Z28_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7715357.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLAARRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(25271..26251)
                     /locus_tag="J1Z28_000493"
     CDS             complement(25271..26251)
                     /locus_tag="J1Z28_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7715358.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIFCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(26251..27273)
                     /locus_tag="J1Z28_000494"
     CDS             complement(26251..27273)
                     /locus_tag="J1Z28_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBA7715359.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            complement(27381..27515)
                     /locus_tag="J1Z28_000495"
                     /pseudo
     CDS             complement(27381..27515)
                     /locus_tag="J1Z28_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303184.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27610..29307)
                     /locus_tag="J1Z28_000496"
     CDS             complement(27610..29307)
                     /locus_tag="J1Z28_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBA7715360.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLHHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLCSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
     gene            complement(29626..31044)
                     /gene="dhaM"
                     /locus_tag="J1Z28_000497"
     CDS             complement(29626..31044)
                     /gene="dhaM"
                     /locus_tag="J1Z28_000497"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBA7715361.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELQAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(31055..31687)
                     /gene="dhaL"
                     /locus_tag="J1Z28_000498"
     CDS             complement(31055..31687)
                     /gene="dhaL"
                     /locus_tag="J1Z28_000498"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBA7715362.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(31698..32768)
                     /gene="dhaK"
                     /locus_tag="J1Z28_000499"
     CDS             complement(31698..32768)
                     /gene="dhaK"
                     /locus_tag="J1Z28_000499"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBA7715363.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGGSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            32987..34915
                     /gene="dhaR"
                     /locus_tag="J1Z28_000500"
     CDS             32987..34915
                     /gene="dhaR"
                     /locus_tag="J1Z28_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBA7715364.1"
                     /translation="MRDMSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGV
                     TFASIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLST
                     LGFNDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRL
                     TGTIALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDG
                     VISWDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQSHPLKHVEA
                     TFESQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDD
                     PQTRRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGD
                     AALAEEFIGGDRTDSENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITR
                     LDARRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPAL
                     VNNKLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSD
                     LPEHLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTL
                     WRKMKQHGIDVSQFKRRG"
     gene            complement(34953..35183)
                     /locus_tag="J1Z28_000501"
     CDS             complement(34953..35183)
                     /locus_tag="J1Z28_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715365.1"
                     /translation="MRLLCDNNHSALTVQEFVMKKTFIFSLLAIVFASLLSACVPHHH
                     HRHNDGPRGPAASGKPMPPSNGGPNHGSHGRY"
     gene            complement(35319..36410)
                     /gene="ychF"
                     /locus_tag="J1Z28_000502"
     CDS             complement(35319..36410)
                     /gene="ychF"
                     /locus_tag="J1Z28_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBA7715366.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(36527..37111)
                     /gene="pth"
                     /locus_tag="J1Z28_000503"
     CDS             complement(36527..37111)
                     /gene="pth"
                     /locus_tag="J1Z28_000503"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBA7715367.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            37389..37667
                     /gene="ychH"
                     /locus_tag="J1Z28_000504"
     CDS             37389..37667
                     /gene="ychH"
                     /locus_tag="J1Z28_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBA7715368.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(37722..39401)
                     /gene="dauA"
                     /locus_tag="J1Z28_000505"
     CDS             complement(37722..39401)
                     /gene="dauA"
                     /locus_tag="J1Z28_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBA7715369.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(39526..40473)
                     /locus_tag="J1Z28_000506"
     CDS             complement(39526..40473)
                     /locus_tag="J1Z28_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBA7715370.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(40624..41475)
                     /gene="ispE"
                     /locus_tag="J1Z28_000507"
     CDS             complement(40624..41475)
                     /gene="ispE"
                     /locus_tag="J1Z28_000507"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBA7715371.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(41475..42098)
                     /gene="lolB"
                     /locus_tag="J1Z28_000508"
     CDS             complement(41475..42098)
                     /gene="lolB"
                     /locus_tag="J1Z28_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBA7715372.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTSDDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            42312..43568
                     /gene="hemA"
                     /locus_tag="J1Z28_000509"
     CDS             42312..43568
                     /gene="hemA"
                     /locus_tag="J1Z28_000509"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBA7715373.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            43610..44692
                     /gene="prfA"
                     /locus_tag="J1Z28_000510"
     CDS             43610..44692
                     /gene="prfA"
                     /locus_tag="J1Z28_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBA7715374.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            44692..45525
                     /gene="prmC"
                     /locus_tag="J1Z28_000511"
     CDS             44692..45525
                     /gene="prmC"
                     /locus_tag="J1Z28_000511"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA7715375.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            45522..45914
                     /locus_tag="J1Z28_000512"
     CDS             45522..45914
                     /locus_tag="J1Z28_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBA7715376.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            45918..46727
                     /locus_tag="J1Z28_000513"
     CDS             45918..46727
                     /locus_tag="J1Z28_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBA7715377.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            46763..47617
                     /gene="kdsA"
                     /locus_tag="J1Z28_000514"
     CDS             46763..47617
                     /gene="kdsA"
                     /locus_tag="J1Z28_000514"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBA7715378.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(47765..47872)
                     /locus_tag="J1Z28_000515"
     CDS             complement(47765..47872)
                     /locus_tag="J1Z28_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic polypeptide LdrA/LdrC"
                     /protein_id="HBA7715379.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(48278..49378)
                     /gene="chaA"
                     /locus_tag="J1Z28_000516"
     CDS             complement(48278..49378)
                     /gene="chaA"
                     /locus_tag="J1Z28_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBA7715380.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGGSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            49648..49878
                     /gene="chaB"
                     /locus_tag="J1Z28_000517"
     CDS             49648..49878
                     /gene="chaB"
                     /locus_tag="J1Z28_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBA7715381.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            50036..50731
                     /locus_tag="J1Z28_000518"
     CDS             50036..50731
                     /locus_tag="J1Z28_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBA7715382.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(50775..51128)
                     /gene="ychN"
                     /locus_tag="J1Z28_000519"
     CDS             complement(50775..51128)
                     /gene="ychN"
                     /locus_tag="J1Z28_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBA7715383.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            51313..52707
                     /gene="ychO"
                     /locus_tag="J1Z28_000520"
     CDS             51313..52707
                     /gene="ychO"
                     /locus_tag="J1Z28_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBA7715384.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(52708..53358)
                     /gene="narL"
                     /locus_tag="J1Z28_000521"
     CDS             complement(52708..53358)
                     /gene="narL"
                     /locus_tag="J1Z28_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBA7715385.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(53351..55147)
                     /gene="narX"
                     /locus_tag="J1Z28_000522"
     CDS             complement(53351..55147)
                     /gene="narX"
                     /locus_tag="J1Z28_000522"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBA7715386.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            55486..56877
                     /gene="narK"
                     /locus_tag="J1Z28_000523"
     CDS             55486..56877
                     /gene="narK"
                     /locus_tag="J1Z28_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBA7715387.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT        14111 a        14030 c        14729 g        14106 t
ORIGIN      
        1 ctcattgatt gacaatattt ttattcaaga agtgtcattg actgttaacg caatgttgta
       61 aaggtaagat aatctgattt atcaatatta ttgtgtgatt gttatgtgag cagaagatat
      121 tcattagcaa cgattacatt agtcatttta ttttgtggac ggcctcattg tcgaaaaata
      181 agcgtacgac agtattatca gaaaagagtg attttttatc caacaacact tcagcgcact
      241 gcgtttaaaa aatgcctctt tcttatacgg gatatcatca tttcatcatg atgtctttga
      301 tgagcggtga acacaataca tttgcgctgt ctcttcagga tgaagcccct ggtctggtgc
      361 ctgcggactg atgttgcagc agagctaagg tcacttagac gctactatca tttatccaat
      421 gcatggaatc gaaatcagtc gatacccgca gtatatatcg tgaactgggc gcaacgctga
      481 gttacaacat gcgtctgggg aacggtatgg aaattgaacc ctggctgaag gcggctatgc
      541 gcaaagaatt tgtcgatgat aaccgggtga aggtgaatag tgacggtaat tttgtcaatg
      601 atttgtcggg cagacgtgga atataccagg caggtattaa agcctcattc agcagtacgt
      661 taagcgggca tctcggggtg ggtataaccg tggtgccggt gtggagtccc cgtggaatgc
      721 ggtggccggt gtgaactggc cgttctgacc atcaacgatt aaactgcgtt tcggcgctgt
      781 ttttgtttac cgggtgctaa aagggaaaat tctggggcaa aaaaaaagcc cgtcagttac
      841 ggcgggaaac ctcatcctat gggagaacaa tgaataatga aattgcgggg ttatcacctc
      901 ccaggataac catactaacg ataaggttat ttgctcaacc aggcgtaaac attttgttct
      961 gtgcgtggga acagccttaa ggtgtaaagg gggaggggga aatagcaatg agtagtatca
     1021 gcaagaatac tcgccgcttt accacaacgt ggatgagagg gatgaaaaac tcaaggcaga
     1081 gatgactctg ccttgaagat aaatgcgctt ttacaggggg cttatttcag ctcttctgct
     1141 tccggtaagg tcacgttcag ctcaagaata gaaatatcgc catctttttg ctcaagctgt
     1201 acggttacca tctcaggatc aatttgtaca tatttacaaa tgacctcaag aatatcttta
     1261 cgcaactgcg gcagataatg cggttctgca tcgctgcgac ggcgttcagc aacaataatc
     1321 tgcaaccgtt cttttgcaat gttggctgtg tttttcttcc gcgagagaaa gaaatcgagt
     1381 aatgccataa cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg
     1441 aagcggaaag gacgttcttc tcccaacaga cgttctacgg tatctgcgta ggctttaccc
     1501 gcatcggcgt taatgtcgag aatgaccggt tcaccctggt tagaggcgcg caatactgat
     1561 tgatcctctg ggatcacgcc gacgagtttg atgcgcagga tctccagcac atcttccatg
     1621 ctcagcatgt cacctctgct tacgcggcct gggttatagc gcgttaacag taggtgctct
     1681 ttaatcggct cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat gcctaaaata
     1741 cggtcagagt cgcgtactga ggagacttcc gggttggtgg taataatggc ttcgtctgca
     1801 aaatagagtg ccattaacgc accggtttca atccctgccg gggagtcaca aacgataaat
     1861 tcaaaatcca tcgctttcag atcatcaaga actttggcga ccccttcacg agtgagggca
     1921 tctttatcgc gagtttgcga tgccggaaga atatagagat tttcagtacg tttatcttta
     1981 attaacgcct ggtttagcgt tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc
     2041 cggcgttcac aacccataat caggtcgaga ttacgcaggc cgatatcaaa atctatcacg
     2101 acagttttct ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta
     2161 ccaacacccc ctttgcccga agtaacaaca ataatgcgtg ccatagaaat tccttgttaa
     2221 aaagggatca atttaacggt tgaacggtca aagcgttttc gactaactgc agtcgtgccg
     2281 ctttgccata aaattctgct gggatttgat cactcagcca gtattcacct gcgatggaca
     2341 ccagttccgc catcaggttc gtacaaaata tttgcgtttc ccggtcacca cttgcccctg
     2401 ccagcgcacg accgcgcatc atgccataga catgaatatt accatcggca atcaattcgg
     2461 cccctgcgct aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac
     2521 cggaacgcac tggggtatca attaaacgcg tttttgtgac cggcgttgta ttttgcgctg
     2581 gagcctgcgg tgcgggagct ggacgcggcg ctttttcctt tccttccgtc aggataggca
     2641 gccccatctt ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa
     2701 cccgcaaacc agttgccgaa accgccttat gcatcgccga ccagtttacc gggtcttcca
     2761 gtgcactgac gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt
     2821 tgtcttccag cgcctgatga ataaccttag gttctgcctc atgcagatga accacagata
     2881 aagtgaagct actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt
     2941 agctattaat catcgccagc gcgcgatgat gttccgaaga ctataaggca tgttatagtc
     3001 tggattatat tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat
     3061 aaatggcgct gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac
     3121 ttttgattgt tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatcttcag
     3181 acctgtttaa atatccagtg ttgaggcgag tgtatttttt caccacgcca ggctttctta
     3241 actttccatt ttttgcagca tcattaaaga acagataccc gttatcttaa tatccgccac
     3301 agaattgctc tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa
     3361 ctcgtccagg tcagcggttt tgattacgga ttgatgatga aatgagcata agtgttgtac
     3421 ggttggatta tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg gattgtcagg
     3481 tgtggggatg atgttctcat caatggcgtc tggaaatgac gctggcggac ttcaatctcc
     3541 ggcgtgcggc gttgtttgcg acccgtatat ttgtgttaac tcagatggca tttccccaga
     3601 gttaacaagg aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcag tacaaggtta
     3661 cgatcccagc gaatttacat tctctaacgg tgtattttgc gatgttaaag aaaaattatg
     3721 tcgtgatgat cgttattttg gtgtgaatgg taagcggagt ggaaaaatca atcaaaccac
     3781 cacaaagatg ttatttatgt gtcgtgagtg atgtcttgtt tacattatgt tctattcgtc
     3841 attaacctct taagcgacag agaggagtgg ggcgttaacg tttatgacgg ggagagtccc
     3901 cgtcggttga tatttactta atttgaatat cgacgttata ttttttagtt ttgtttttac
     3961 gggcgtcgtt tcgggtttgc agcactcgca gctcatattt gccagaagca gggagtgagt
     4021 attgtccgtg actatcgagt tctggggaat atctggataa atcaacagaa tcatcgattc
     4081 ctggcccgaa gaggtaagta tcagcgcctt cattagagat actcacgtgc actttctgac
     4141 cttttttggc atagaaggta tatgtatcgt aatcgtatcc ctttatttcg ccggagtact
     4201 gagcgctgct atggcctttt ctgaactcaa cattgacatt cttacccgca gcaaagctcg
     4261 tggaggttag cagtgcaagc aacaatacag ccttactgat gcccttaatt ttcattggta
     4321 gtctcctgtt cagaatgagt acgatgaact gttataatat aacaatcctt cacgggagaa
     4381 ttccagtggc tttcctgtgt tcggttaaat cccggcgcgg catttttgcc ggggggatta
     4441 cttataaagg gggcatggac tgaacaaacc gtagaaatct acagctggct ggcttgtggc
     4501 gcgggtttca ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt
     4561 tttgtgtgat ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag
     4621 acgatttttc gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga
     4681 tgattcttcc gctggatggg cggaagaaac tggtcaatgc cgatattgaa aaggttaaac
     4741 aggcattaac cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga
     4801 agcaacatct ttccgtcatg gggcagaaaa cagacgacac gaacaaataa ccgatatccg
     4861 gcggtggcat tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt
     4921 tatgtatcaa catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt
     4981 ctgtgttggc agtaactatg ccaaacatat taaagagatg ggcagcgcag tacccgaaga
     5041 gccagtgctg tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat
     5101 cccatccgat ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac
     5161 gctgcgtcag gctacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct
     5221 cgatctgaca ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa
     5281 ggccaaagcg tttgataact cttgtccgct ttccgggttt attcccgcgg cggagtttac
     5341 cggcgacccg caaaatacaa cgctgggcct gagcgtaaac ggcgagcaac gccagcaagg
     5401 tacgactgcg gacatgatcc ataaaatcgt tccgctgatc gcttatatga gcaagttttt
     5461 taccctcaag gccggtgacg ttgtgctgac aggcacgcct gatggtgtcg gcccgttgca
     5521 aagcggtgat gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta
     5581 atactttttg ccgcctgaaa gcggcggcaa aacttgcatc gctgtgccag actggttata
     5641 aggtgcgttt taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta
     5701 aaaccctgga cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt
     5761 gttgcctgca taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct
     5821 gtcgccagct caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg
     5881 aacccgactg cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga
     5941 cctgcgctta tcgtttgctg gcggaaggta aaggtttacc tgcgtggcat ccgctactta
     6001 ctggttcgaa agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtaaaag
     6061 aatcagaagt gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat
     6121 aaatttaata gatgttgatg ggggctttct taattcacgt ctggatgctc gccatttcgt
     6181 agccctgttt ttgaaagcgc tctgtcataa tgagagttac tcgatacctt attatcaaca
     6241 ggtaactctc aatctttaaa aaacgtctgt atttttaaat gtgataaact ttataccttg
     6301 agtacataga gagaataatc gcttatcaga cttcagatac ctcaaagagt gtcttcttac
     6361 cgtgtctttt ctgatactca ttacaggtgt taattatttt tttggccgct tcttttagta
     6421 aagaaagcat taaatcatca taatcaacgt agaatctggt tgtttcagtt tccgttttta
     6481 tctccccgat ggcggctatt tcggtagtta atttcaattt ggcggcatcg atatctttat
     6541 tcgcttgttt aaccgtgtta gacagggtgg taaagaaact ctgcgcagat tttaacttgt
     6601 tcttcaattc tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa cacagtttct
     6661 tgatgccgtc atccagtacc ttaatgagaa tgcctttctg ggcgaatgct tcgattctat
     6721 tttaaaccag gtgatatttt tcctttctgc tggatggacg ctgtcgccag aaggccgcgt
     6781 gagcacagga taagagaacg aaaaatcagc agtctatgca gcgacaaata ttgatagcct
     6841 gaatcagtat tgatctgctg acaagaacag actactgtat ataaaaacag tataacttca
     6901 ggcagattat tatgttgttt atcaagcctg cggatctccg cgaaattgtg acttttccgc
     6961 tatttagcga tcttgttcag tgtggctttc cttcaccggc cgcagattac gttgaacagc
     7021 gcatcgatct gaatcaactg ttgatccagc atcccagcgc gacttatttc gtcaaagcaa
     7081 gtggtgattc tatgattgat ggtgggatta gtgacggtga tttactgatt gtcgatagcg
     7141 ctattaccgc cagccatggt gatattgtca tcgctgctgt tgacggtgag tttacggtga
     7201 aaaaattgca actacgcccg acggtacagc ttactcctat gaacagtgcg tactcgccca
     7261 ttaccatcag tagcgaagat acgctggatg tctttggtgt ggtgatccac gtcgttaagg
     7321 cgatgcgctg atgtttgccc tctgtgatgt aaacgcgttt tatgccagct gtgagacggt
     7381 gtttcgccct gatttatggg gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt
     7441 tatcgcccga aacgctgagg caaaggcgct gggcgttaaa atgggcgatc cctggttcaa
     7501 acaaaaagat ctgtttcgtc gctgtggcgt ggtttgcttt agcagcaatt atgagcttta
     7561 cgcagacatg agtaatcggg tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat
     7621 ttacagtatt gatgaggcat tctgcgatct gacaggtgtg cataattgtc gcgatctgac
     7681 tgattttggc agagaaattc gcgcaatggt gctacaacgt acccatctta ctgttggtgt
     7741 ggggatcgcc cagaccaaaa cgctggctaa gcttgccaat catgcggcaa aaaaatggca
     7801 gcggcagacg ggcggggtgg tggatttatc aaatctggaa cgccagcgta aattaatgtc
     7861 tgctctcccc gtggatgaag tctgggggat tggacggcgg atcagcaaaa aactggacgc
     7921 gatggggatc aaaaccgttc tcgatttggc ggattcagat atccggttta tccgtaaaca
     7981 ttttaatgtc gtgctcgaaa gaacggtgcg tgaactgcgc ggtgaaccct gtttgcaact
     8041 ggaagagttt gcaccgacga agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat
     8101 cacggattat ccgtcgatgc ggcaggccat ttgtagttac gctgcccggg cggcggaaaa
     8161 acttcgcagc gagcatcaat attgtcggtt tatctccacg tttattaaga cgtcaccatt
     8221 tgcgttcaat gaaccttatt acggcaatag cgcatcggta aaactgctga cgcccactca
     8281 ggacagcagg gatatcatta acgccgctac gcgatctctg gatgccatct ggcaagcggg
     8341 ccatcgctac caaaaagcgg gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca
     8401 gctcaattta ttcgatgaca acgcaccgcg ccccgggagt gagcaattga tggcggtaat
     8461 ggatacgctg aatgctaaag agggcagagg aacactctat tttgccgggc aggggatcca
     8521 gcaacaatgg cagatgaagc gtgccatgct ttcaccatgt tatacaacgc gaagttctga
     8581 tttactgcgg gtcaaataaa catagcggca ggaaaaagcg ctcccgcagg agcgccgaat
     8641 ggattagcga ccgaacaggt cacgtttttt cgctttaaac ggctgggaaa tcaccaccag
     8701 caccgcgaca atcaggtaag cgataaaaat accgagcagc cactgcggca tttccagacc
     8761 taaaaattcc cactgacgct cggcgcaatc gccagaggcg acaaacactt gcggcaccca
     8821 tttatccagc ggcagccatt ccgggaaacg agccataaaa tcacaggtcg caaacggcga
     8881 aggatagagc tgaagcatgg tgtgctcgta agttaactgc acaccgcgga acgcactata
     8941 caaccagata accatcgcta cataacgcag cggagttttc ggggcgatcg cgccaatcag
     9001 cgcagcaccc agaacgccga ataacgcgca gcgttcataa atacagagca cgcaaggttt
     9061 cagtaacatc acatgctgga accacagcgc cgtcagttcc agtgccagag cagtaaacgc
     9121 catcaacagc catgcgcccc ggccttgtga acattggttc aaaaatcgca acataatcat
     9181 ttccctgcaa tatgcataga gtgcgcagtt taaaccaatt cattcgctgc gccaccagga
     9241 ggcagcaaaa ataggacgca attggacaat tatcgggcga aaaggcaaac cgggcattta
     9301 tatgcccggt aagttgttat caaagcgttg ctatccagcc catttgcata aaccattcgg
     9361 ttacaggagc aagcgtaaac tcgacgcaga gcagcccgac gagtgtcagg acgagggtgt
     9421 aaggcagggc catccacacc atgcggccat aagagagacg aatcaatggc gcgagtgcag
     9481 aggtcagcag gaacaggaac gcagcctgac cgttcggcgt agcgacggag ggcagattgg
     9541 taccggtatt aatggcgacc gccagcagct catattgctt caacgtgata gcgccacttt
     9601 ccattgccgc tttcgcttcg ttgatataaa tcgtccccac gaagacgtta tccgaaatgg
     9661 acgacagcag accgttgaaa atatagaaca acgacagctg agcatgttca gatgcctgca
     9721 acacaaactg aataatcggc gaaaacagtt gttggtcgat aatcaccgcg actactgaga
     9781 aaaacactgt caacagtgcg gtgaatggca gagattcggt gaaggcttta ccgatagcat
     9841 gttcatcggt gacaccggtc aatgatgtcg ccagaataat gaccgacaaa ccaatcaaac
     9901 caacttccgc cagatgcagt gccagcgcag tcaccagcca gacgccgata atcgcctgga
     9961 caatcagacg gattttatcc tgacgggtac gctggttgcg gctttgatcg tcaaactgtt
    10021 gcagtacttc gcggactttc tccggcagcg tttcaccgta gccaaaccaa cgcagcttct
    10081 ctaccagcag gcaggttaac aggccacaaa tcagaaccgg gacggtcacc ggcgacatgc
    10141 gcaggaagaa atcgccaaaa tgccagccag ccgctttagc gatgatcagg ttctgtggtt
    10201 cgcccaccat ggtcatcacg ccgcctaatg cggtgccgac accggcatgc atcatcagac
    10261 tacgcagaaa gccacggaac tgttccagaa ccactttgta atgcttgtcg atatgactat
    10321 cgtcttgcag gtcggtatct tcagtacggg aagaggctac gcggtgataa ataccataaa
    10381 aaccgactgc aacgctgatc accaccgcca cgacggttaa ggcatcgagg aacgcggaga
    10441 ggaacgccgc cgccacgcaa aaagagagcg acagcagcat tttggagcga atacttaaca
    10501 gcaaacgggt aaatatgaac aacaacagct gtttcataaa atagataccc gccaccataa
    10561 acatcagtaa cagcaagact tcaagatttg ccgccacttc ttcacgaaca tgttccgcgc
    10621 tggtcatgcc gatgaatacc gcttcgatag ccaacagacc accggggagc agcgggtagc
    10681 atttcagggc catcgccaga gtgaaaataa attccgctac cagcaaccag ccagcgacga
    10741 aagggctgat gaggaaaatt aacggattta cgattaagaa aatgatgagg gcgagtttgt
    10801 accagtcggg ggactggccc aaaaaattgc gccatagtgc gcggccccag gagatctcca
    10861 tgatggtttc ccttacctta gaaataatca atgatgtttt tatgtttaaa cgcaaagctt
    10921 aacggtcaga caggagtgag gcaagtcttg atagtcaagg ggaaagagat gcagaaaatg
    10981 aagccttgat cccttttttc ttctttttgt cttctatcag cgtagttagc cctctggtat
    11041 gatgagtcca actttgtttt gctgtgttat ggaaatctca ctatggtcat taaggcgcaa
    11101 agcccggcgg gtttcgcgga agagtacatt attgaaagta tctggaataa ccgcttccct
    11161 cccgggacta ttttgcccgc agaacgtgaa ctttcagaat taattggcgt aacgcgtact
    11221 acgttacgtg aagtgttaca acgtctggca cgagatggct ggttgaccat tcaacacggc
    11281 aagccgacga aggtgaataa tttctgggaa acctctggtt taaatatcct tgaaacactg
    11341 gcgcgactgg accacgaaag tgtgccgcaa cttattgata atttgctgtc ggtgcgtacc
    11401 aatatttcaa ctatttttat tcgcaccgcg tttcgtcaac atcccgataa agcgcaggaa
    11461 gtgctggcaa ccgctaatga agtggccgat cacgccgacg cctttgccga gctggattac
    11521 aacattttcc gcggcctggc gtttgcttcc ggcaacccga tttacggtct gatccttaac
    11581 gggatgaaag ggctgtatac gcgcattggt cgtcactatt tcgccaatcc ggaagcgcgc
    11641 agtctggcgc tgggcttcta tcacaaactg tcggcgttgt gcagtgaagg cgcacacgat
    11701 caggtatatg aaacagtgcg tcgctatggg catgagagtg gcgagatttg gcaccggatg
    11761 cagaaaaatc tgccgggtga tttagccatt caggggcgat aatcccttcc gtttaaaaag
    11821 caaacccctc aaacgagggg ttttcattgt ttttacagat ttcccattct cggcgggcaa
    11881 cgttccagca actcgacgct gccgtcttcg ttttgctgtt ccagcatcac atcaaatccc
    11941 cacaggcgat gcacatgctt cagcacttct ttgcgccccc gatccagcgg tgcgcgatta
    12001 tgtggaatgt aacgcagcgt cagcgaacgg tcgccgcgca aatccacgtt ccagatctga
    12061 atattcggct ccagattact taagttatat tgcgatgata accggttacg gatttcccga
    12121 taaccttctt cattatgaat agcggaaatc tccagataat tatgccgatc atcatccagc
    12181 acggtgaaga agcggaaatc acgcatcact ttcggtgaca ggaactggct gataaaactc
    12241 tcatctttga aatcacgcat cgcgaaatgc agcgtttcca gccagtcgga accggcgata
    12301 tccgggaacc agtatttgtc ttcttccgtt ggcgactgac aaatccgttt aatatcctgg
    12361 aacatggcaa acccgagggc atacgggttg atgccgctgt accacgggct gttatagggc
    12421 ggctggaaga ccacattggt gtggctgtgc aaaaactcca gcataaaacg ttccgttact
    12481 ttcccttcat catacagatg gttaaggatg gtgtagtgcc agaaggtcgc ccagccttcg
    12541 ttcatcacct gagtctgttt ttgcggataa aaatactggc tcaccttacg cacaatacgc
    12601 aggatttcac gctgccatga ttccagcagt ggcgcatttt tctccataaa atagagcagg
    12661 ttttcttgtg gttcggacgg atagcggcgc gcttcagcaa ccgttttctc ttcctcgcgc
    12721 ttcggcaggg tacgccagag catattgacc tgactttgca gatactcttc gcgactcttc
    12781 tgccgggctt tctcttcttg cagcgagatt ttttgcgggc gtttgtaccg gtccacgccg
    12841 tagttcatca gcgcgtggca cgagtccaga agccgttcta cttcatcaac gccataacgc
    12901 tcttcgcact cggtaatata tttacgggca aaaatcagat aatcgacaat cgaactggcg
    12961 tcggtccagc tacggaataa gtaattgttt ttgaagaaag agttatgccc atagcaggca
    13021 tgagccatca ccagcgcttg catggtaatg gtgttctctt ccatcaggta agcgatacac
    13081 gggttagagt taatgacgat ttcataggcc agtccttgct gaccgtgctt atacagccgt
    13141 tcagtctcga taaacttttt accgaatgac cagtgcggat agttaattgg catgccgacg
    13201 ctggagtagg catccatcat ctgttctgag gttatcactt caatctggtg cgggtaggta
    13261 tccagccggt agagtttcgc cacccggtct atctctgcca gataaacatc cagcaggtcg
    13321 aacgtccagt cgggtccatc gctcaaacgt gtggtgtcct tattcataga atcgatcgtc
    13381 gccatacgcg cacctcattg ttgtcggcgc tctctgtgtg gagcacctca tttcaagcat
    13441 agaacacctg ttaaaaaccg cgtcgccgga gaattttttt ctttgcgatt tcttattatc
    13501 agagtgccac taatccgctt ctgaacggaa ttttatgctg gataaaaagg gcgttcagca
    13561 ggagatacta aagacgccat attgccgcag agtcagggag atgtgagcca gctcaccata
    13621 aaaaagctgt atgttgaata atattttcaa ctgagttatc aagatgtaat tagattattg
    13681 ttcttttact gtatctaccg ttatcggagt ggctatgcga gttgtcatac tgggaagtgg
    13741 tgtggtaggc gttgccagcg cctggtactt aaatcaggca ggacatgagg tcaccgtcat
    13801 tgatcgggag ccgggggccg ccctggaaac cagtgccgcg aatgccgggc aaatctcccc
    13861 cggatatgct gcaccgtggg cggcaccagg tgtgccttta aaagcgatta aatggatgtt
    13921 ccagcgccat gcgccgctgg cggttcgtct cgacggtacg cagttccagt tgaaatggat
    13981 gtggcaaatg ttacgtaact gcgacaccag ccactatatg gaaaacaaag ggcggatggt
    14041 gcgtctggcg gaatacagcc gtgattgcct gaaagcattg cgcgccgaaa ccaatattca
    14101 gtatgaaggg cgtcagggtg ggacgctgca actgttccgt accgaacaac agtatgaaaa
    14161 cgcgacccgc gatatcgccg tgctggaaga tgccggcgta ccgtatcagc tgctggaatc
    14221 cagccgcctg gcggaagtgg agcccgcgct ggcagaagtg gcgcacaaac tgacgggcgg
    14281 cctgcaactg cccaatgatg aaaccggaga ctgccagctg tttacccaga atctggcgcg
    14341 gatggcggag caggcggggg ttaaattccg ctttaatacg cccgttgacc aactgctttg
    14401 cgacggcgag caaatctacg gcgtgaagtg tggtgatgaa gtgattaagg ccgatgcgta
    14461 tgtgatggcg tttggttctt actcgacggc gatgctcaaa ggcattgttg atattccggt
    14521 gtatccgctg aaaggctact cgctgactat tccgattgcg caggaagatg gtgcgccggt
    14581 gtctactatc cttgatgaaa cctacaaaat cgccattacc cgtttcgata accgcattcg
    14641 tgttggcgga atggcggaaa ttgttggctt taacaccgag ctgttgcaac cgcgtcgtga
    14701 aacgctggag atggtggttc gcgatctcta tccacgcggc ggtcatgttg agcaggcgac
    14761 tttctggact ggcctgcgcc cgatgacgcc agacggcacg ccggttgtcg ggcgtacacg
    14821 ctttaaaaat ctgtggctga ataccggtca cggcacgctc ggctggacga tggcttgcgg
    14881 ttccggtcag ttgttaagcg atctgctctc ggggcgcacg ccagcgatcc cgtatgaaga
    14941 tctaagcgta gcgcgctaca gccgtggatt tacgccatca cgcccgggcc atttacatgg
    15001 cgcacacagc taaggaaacg agatgacccg tccgatacag gccagcctcg atctgcaggc
    15061 attaaaacag aatctgtcca ttgtccgcca ggccgcgccg cgcgcgcgcg tctggtcggt
    15121 ggtaaaagcg aacgcttacg ggcacggtat tgagcgtatc tggagcgcgc tcggggctac
    15181 cgatggcttt gcattgctta acctggaaga ggcaataacg ttacgtgagc gcggctggaa
    15241 aggaccgatc ctgatgctgg aaggattttt ccatgctcag gatctggaga tttatgacca
    15301 gcaccgcctg accacctgcg tacacagcaa ctggcagctc aaggcactgc aaaatgcgcg
    15361 gctaaaagca ccgttggata tttatcttaa agtgaacagt gggatgaatc ggttgggctt
    15421 ccagcccgat cgcgtgctta ccgtctggca gcagttgcgg gcaatggcga atgttggcga
    15481 aatgaccctg atgtcgcatt ttgccgaggc ggaacatcct gatggaattt ccggcgcgat
    15541 ggcgcgtatt gagcaggcgg cggaagggct ggagtgtcgg cgttcgttgt ccaattcggc
    15601 ggcgactctg tggcacccgg aagcgcattt tgactgggtt cggcctggta ttattttgta
    15661 tggcgcttcg ccgtccggtc agtggcgtga tatcgccaat accggattac gtccggtgat
    15721 gacgctaagc agtgagatta ttggtgtcca gacgctaaaa gcgggcgagc gtgtgggcta
    15781 cggcggtcgc tatactgcgc gcgatgaaca gcgaatcggc attgtcgccg caggatacgc
    15841 cgacggttat ccgcgccacg cgcctaccgg tacccctgtt ttagtggacg gcgttctcac
    15901 catgacggtg gggacagtct cgatggatat gctggcggtc gatttaacgc cttgcccgca
    15961 agcgggtatt ggtacgccag ttgagctgtg gggcaaggag atcaaaattg atgatgtcgc
    16021 cgccgctgcc ggaacggtag gctatgagtt gatgtgcgcg ctggcgttac gcgtcccggt
    16081 tgtgacggtg taacttgtta tcgctggatg cgaccagcgt cgtatccagc gttataaaac
    16141 acattaaact taagattcgg cttcttcctc cgctacccga acaccaatct tcaacacttc
    16201 attatcttct ttctcggcca ccgtccagat catcccggca aactctacct ggtcaccgac
    16261 aaccggtgct gcgcctaaca actgctggac gatttcaccc agcgtttgct gcttatcgcg
    16321 atattctcgc ccgtcttcca gaccatatat cagcgccaca tcagcatatt tggcgctggc
    16381 ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggcggcg actggctgaa
    16441 cagtttaccg agcgccggga gatcgcgttc ccgaccaatt acacacaaca catcgccttc
    16501 gcgcagtcgg gtgctgccgg tgggatgaag caactggtta tcacgaaaca gtgccgcaat
    16561 acgcgtctct tttggcatat gcaaatcacg cagtgccgcg cccacgcacc atttatcggc
    16621 actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca ggccaacgcg
    16681 tgacaccgga cgtcccactg gcggaacgac cactttggct tttttcgccg cccacgagag
    16741 tgatgttccc tgcaatagca gtgaaacaag gaccacaaag aaggcgacat taaagaacag
    16801 tcgcgcattc tccagccccg ccatcatcgg gaacacagcc agaataatcg gcaccgcgcc
    16861 gcgtaatcct acccagctga taaacacgcg ctcacgcaga ttgaagccac ggaacggtag
    16921 caatccggca aataccgaaa gaggacgggc gaagaaaatc atccatgcgg acaaaatgag
    16981 cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca gcaccaggaa
    17041 catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt
    17101 gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa taccgctacc
    17161 ttccagcgcc gtggttaaag caaaaattaa aattccgccg cttaatgcca gcaatggata
    17221 taatccggcg ggcagggcga tgcgattaat catttgcagc agtaaataac caccgccaag
    17281 cccgatgaca attccgaggc caaattgctg cagaatatcg accacgaaca tccagctgac
    17341 actgctttca tgctgctgga tcatcgcaat cagggtaatc gtcagaaaga ccgccattgg
    17401 atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa gccccttacc
    17461 acccagcaga gaaaagaccg ctgcagcatc ggtggagccg acgatagcgc cgattaataa
    17521 tccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta aaccagaggt
    17581 gataagcacg cccagcgtcg ccagcgacag tgccggtcct aacgccacac gaaaggaact
    17641 ggcctgagtg cgcatcccac cgtcgagcaa aataatcgcc agcgccaggt tacttaccat
    17701 gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcaaccc ccgccagcat
    17761 gccgatcgcc agaaaaataa ccagaatggg aatgccaaga cgggaagaaa atgaactaag
    17821 taaaatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta ttgttgtggc
    17881 atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac catattagcg
    17941 gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta agaacaggat
    18001 gaccgctaat cgtcaactga gtgccttcct gggtgttatt cagaatggca tgtgcgccca
    18061 acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg agtggaatcg
    18121 acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg tcataatcat
    18181 taggcgtgct gccgctaaag ctaccgagaa taatcgcctt ctgacgtggc aaaattccag
    18241 catgatagag ctgcaacagc atacgttcga cgcggaaagg gtgctcgtta atatcttcca
    18301 gcaccagaat accgttctca atttttggca tccacggtgt accaatcagt gaaatcagca
    18361 tcgcaagatt gcctccccac agcgtgcctt cggtctgaca tgtcgggcct tccccttgcc
    18421 attcaatggt gaacgtttta ttgcgtaacg ccagccagaa gtggtgctcg gtaaaggcat
    18481 ttagttcatc cgcgccaaag tttgctacca gcatggggcc gctaaaggtg atgacattgc
    18541 cttgcgccag aagaccgcac tgaatggcgg taaaatcgct atgtccgcaa atgagtaacg
    18601 ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt aaacgactgg
    18661 caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtagtcagt ctggcaagag
    18721 aattgagatc ttccagacgc tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa
    18781 taacctcgac gttattgacc tgatgccccg cgtcggtaag gcgctggata ccacgcagcg
    18841 cggcgtgctg tttaatgcag taacccgatg gggcaattaa gtgaaacaga gacatggcaa
    18901 ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc
    18961 tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt ttgccttttt
    19021 gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc cgtggaacgc
    19081 gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag ccggtgcagc
    19141 ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg gtggtaatcc
    19201 gaacgcggtg agtaaatcga atgccattgg tttgatgcag ataaaagctt caacctccgg
    19261 acgtgatgta tatcgtcgta tgggctggag tggtgagccg acgaccagcg agctgaaaaa
    19321 tccggagcgt aatatttcaa tgggagcggc ttacctgaat attctggaaa ccggcccgct
    19381 ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat acgctaacgg
    19441 ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa
    19501 cgatctggat gctgatgagt tcctcgatca cgtagcgcga aatcatcctg cgccgcaggc
    19561 tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca gtcgcgcaca
    19621 ttgtccgctt ttttccgggc ttctcgctcg agagagaaaa taatccgcta taattgccgc
    19681 tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt gatggtttca
    19741 tttttgccat caatcacttt gcgctcgctg atggagatta actgggcgtc aaagtgaaaa
    19801 acaccccatt gccccatgtt gacttcaatc tgagcgaagc gcatgccttc gtgtaattcg
    19861 gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc atacaggcgg
    19921 aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg atggagtggg
    19981 gcggtgatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg cggtacggta
    20041 ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt aaactcgact
    20101 ttcgcaccct gagtttcggc ggtaatggta atgtgctgcg cctttagcac ggcgatgttg
    20161 tcttcggcct ggctgccgaa atccagcacc agtttatccg gagttattgc cagtattttg
    20221 ctgatcagct gcccgccatt ccagctgata cgcaaaggaa ttgcggcttt gtgcaaatcg
    20281 cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg gtaatgactc
    20341 acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcgcag ttaacaaaac
    20401 ttaatacaaa tgcgcgaata ttttttacat gttgttctta aatcagccga catacgccca
    20461 gcatcgatcc cgcccttgcc tatacttaga gcgttgacgt aagcatttct tgcgtccgat
    20521 tcatcgaaac gagggcctga acatgggaat tattgcctgg attatttttg gcctgatagc
    20581 cggtattatc gccaagctaa tcatgccggg gcgtgatggt ggtggatttt tcctgacctg
    20641 tattcttggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt ttggcattgg
    20701 cggctccatc agcggtttta atttgcacag cttcctggtg gcggtggtgg gcgctatcct
    20761 ggtgctgggc gtattccgcc tcctgcgaag agaataagat tttcataagg cggatagcga
    20821 tacagatgcc gctatccgct ttcacatcag aacgtatact cgataccagc ccagaaattg
    20881 cgaccttctt caataaaccc ttcactgtag gcatatttgg tatcaaacag gttattgacc
    20941 gacgcattaa cgctgaagcc atgacctaag gtgtaatcgg ctcgaatgtg ggtcaccgca
    21001 aaaccggcgg ctttttgtga accgtcactg ttgctgtagc tggaggaacg cgcctcttcc
    21061 gacagcgtta cgcttaacgg ctcccacggt ttgagagtca tccatgcggt cattgtctgc
    21121 gttggcagat cggttatctt gccgatgtct ttacgtttgg catcagcgtg gatcagggcg
    21181 tagctcaatc ctacatccag tatattgctg attttcccct taataccggc atccagaccg
    21241 ctgtaatcca ccgtgccgct gttctgattt tgaatggtat cggcatcgat attgtgcgag
    21301 aggatggcat cactcacccg gttatagtaa acgctgacct caaatcccca gtcgtgcgtg
    21361 aaggcaccat tccaggttaa atccaccccg cgcgcgcgtt ccggcttgag ctgcgggtta
    21421 acaatcgcta tctggttata cgcaggtttg gacgtggtat agcgttcttt cagcgtcgga
    21481 aagcgtgtgc ggtcatagta cgaaagcgcc agcgtgtctt cattggcaaa gtggtatttc
    21541 cccatcacct gccagttaaa agctgactga ttgttgtcgt cataatgggt gatactgcca
    21601 tctttctcat gttttttcgc ttctacgcta tcgcgccagt catagctgat tccagccacg
    21661 acatcgacat tatcggcagc agcccattga tattcactgg cgagcgacca ggtacgatct
    21721 tcatagcgat cgtaagcggc gtgcggcgca cctttttccc ggtgtacgtc atctttccag
    21781 ttaacggcaa acgacagcag atcgttttca cgcacatcgg ctgccagttg taacccggca
    21841 ccgtcgctgt aatcggaata atggctgtag ctgccttttt tatttttcaa atcagccagc
    21901 gagttgtaca tcattagcgt attttcaaag gtgtcgcgat acagccgact tttcagggta
    21961 aaacgatcgt ttagttgggt cgttccctga taataaaaac tttctttgtc atactctggc
    22021 cactgccagt agcgtgattt ctgaccacta tttccactgt atggcgggtt atctttttca
    22081 ccatcctgct taatgtaagt caatgtgtat tcatcgtttt cgcgtggtgt aaaaccgagc
    22141 ttcacaatgc cgcgtttatc atcagccgat gaattaatca tcttgccgtg tttgcctgca
    22201 atatcattat ttacaccatg cggcaggccg agaaaatcct gctttagctg gctaccgctg
    22261 atttgcaaat accccagatc gctgctggcg gcaaatgaag catgcatatc gtaggcattg
    22321 tcctggctac ggctccatcc ctggcgatat cccagatttg cttccagagg ttttgttggc
    22381 ttctgggtgg tgatattaat ggctccgccc atctgattag gcccctgaag cagcgacgaa
    22441 taccctttgg aaacttcaac tgcccccaaa ttgttggtca gaatccgcgc cagatcgagg
    22501 ttgccgtcat agggaacata aatgggcaca ccgtcgaaat agaccggcac ctgacgacta
    22561 tcaaagccac gaactttgac ctgttcttcg ttgcggctac ctgacttttg cagcaccacg
    22621 ccggggacga cacttaatgc ctgagcgaca ttctgtttat ccagggcctt catggtgggt
    22681 tgatcgagaa cggtggtcgt cgttgatgat accggactgg accatacggt cagcgtgtca
    22741 ctctcctgag ccgctacgga ctgctgggta aaggcaagtg ataatactgt gcagagataa
    22801 cgttttttga gtctcataga ataaattccg tttaacaaag aattaaatag aggctgcggg
    22861 ttgcagactg actttaatgt cagcaggtgc cgcgtaataa ggcgcggagg taataaaaag
    22921 gcgaatgccg cagtcgaggt aatttttgag tgttgtcaga ttaattccgc cggtcagagc
    22981 cagcgtgcag tggggagcca gcgacggtgc tatttgcgca atttcagttg cctgttgcgg
    23041 actaaatttg tcgagctgaa gtacgtctgg ttgcgcgcgt aacgcggcga ttgcctcttt
    23101 cggcgtgtcg gcttcaacaa ctattttctt ctcgggtgcg tggcgacgta actgattgat
    23161 tgcgcctgac cagtcctgat tgtcatgaag aaaatggcgg tggttggcaa acaataatat
    23221 ggtttccgca catccggcac gatgaatcag tcctccggca gccagaattg cctgcgaggc
    23281 cagtaagcgg gtgcccggaa ttgcttttcg ggtgcaggcg atattgccat cagggtaacg
    23341 ttcacgaagt aacgccagca tttgagcgag ataatcagaa acaccgcaac tccactccag
    23401 cacattctgg actgccttcc atccctgatg aagtgctgcg gcattacctt gcgcacggat
    23461 tagccgctga cccgcgttcg cttgtgaacc gtcgctgacc gcgttatcaa tagttaatcc
    23521 cagcgtagtt aacatcttac acgcgacaga aattccgctg acgcagccgc cctgacggtg
    23581 aaaaaactct atatagccat gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc
    23641 gccctggata tcttccagaa gtaacgcatc gatttgtgcc tgagaaagga aaatcatcgc
    23701 agtgtctcct gcggtacgct atcccaccag atgagcgccc agtcgcggct ggcaggggcg
    23761 agggcacgag catcttgttg ttgataccag cgatataacc gctcacgctc atcgtcgtta
    23821 agcgcgccca gactccagct gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt
    23881 tggcaatttt gtccacggat aaaatcaacg tgagcataaa ttcccatctg atacaggacg
    23941 ttgagtgcaa agatatagtt gggtaattcg atcacttccc gaccagccgc ccgctgaatg
    24001 gctggagaga cgaacgaggt gctgaccaga tgagtcgtat agacgcgaag ccgtgcctga
    24061 ttattcagtt tgctcattgc ctggcgcata tcagccacca gagtggagcg ggaagccacg
    24121 gcaatatcgc agcgtggcag gtcactccag tcctcttccc aggcgcgctg gatccagtgg
    24181 acattgtctg cctttagcgc ggcggcgcga cgggcggcaa cgttcagcat cccctgactg
    24241 taatcaacgc cataaatcgt cgtgagtttg tccgccagcg ccagcgatac ggtacccggt
    24301 ccgcagccca tatcgaataa ggtttgcgcg ccttgcaggt caatttttga ggtcagttgt
    24361 tgcaggtagc tgtccgtcgg cgaggcgcaa ttttccgcca ttttttccgc ccgttgatcc
    24421 cagtgatccg gcgttttttc tgtgcgatgg gccagcctga gttgctgaag gtaaaggtct
    24481 gcaaaatcaa tatcgtcaat gagcatgatt tttccttagt gcttaattgc agagaggtga
    24541 tggtgaatct ggtcggcaga aacccggtac agggcagcca gttttttcgt tgttaactgt
    24601 tcggtcggta agccttgtgt tactcgtcca tctggctcta cctggataat gctgtcggca
    24661 atggcgttgg catggagcgg atgatgagtc gacattaaca ttgtcatgcc gttatttttg
    24721 agctgcgcca gcgtgtccag aagttggatc tgatgaccga aatcgaggct ggaggcaggc
    24781 tcatccagta ataacaagcg cggttgctgg acgagtgcac gggcgattaa agccagctga
    24841 cgttcaccgc cgctaagcgt attccagcgt cgtgccgcca gatgcaaaat gcccagcttt
    24901 tccagttgct ccgttgcttt caggcgttct tgcttaccgg gaacggaaaa agcgcctatg
    24961 gtgggggcca ggcccatcaa caccatatcc agcacactaa aagcaaaaat gccgtcgtgc
    25021 gcctgcggca cccaggcaat agcctgcgcg cgctggcgat ggctgagtgt ctgcacagag
    25081 aggctatcca gtaagatctc gccttttaat aacggtataa cgccaagaat ggagcgcatg
    25141 agtgtggttt tgccgcaacc attggctcct aacagacaac aaattgttcc ttgcgacagg
    25201 gtgaaggaca cgtcgcgtag caccggatgg cgggagtaac ccagagacgc atgacggacg
    25261 gcaagcaaag tcactggcga cctccgcgta acaacaaaaa caggaaaaac ggtgcaccaa
    25321 caaaagcggt cagaatgcca agtgggattt ctgttgttcc aatgctgcgt gccagcgtat
    25381 cggtaagcag gagcagaatc gcgccagtac acattgccat gggcaacagc tgctgatggt
    25441 tatgaccggt caacaagcgg gcaatgtgcg gaacgaccag gccgacccaa ccaattatgc
    25501 ccgctattgc taccgtgctg gcggtgatga gtgtggcgca gaaaattaat ccaaagcgca
    25561 gacgtgtcac gttcagtccc agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca
    25621 tgcgccagcg gagaaaaaac aggggcaagg agcctatgag aatgatcggt gcagcatagc
    25681 agagatcgcg taaggtcacg gtggacagcc cccccagcaa ccagaaggta attgacggta
    25741 actgggtgta aggatcggcc agcgttttta ttagcgatat ccccgctccg cacaacgttc
    25801 ctaacgcaat gcctaccagc accagggtta agatcggatc gtggcgggtc acccgacgcg
    25861 taatcaacca gacgcccgcg accaccatca gcccgccgca aaatgcgtac agctggatgt
    25921 aaacaatgga taatccccac aggattgccg cgcaggcacc aagtccggca ccggcggaga
    25981 cgcctaaaat atccggtgac actaagggat tacggaacat tccctgatag gtggttccgg
    26041 cccctgccag cgccgcgccc agaaacagcg cagcaagaat acgcggcacg cggatttgcc
    26101 agaagacaat ttgttcttgc acaggaacat tttcttgacg aagaatgagc gccagcagtt
    26161 gctgcatatc gagatgataa gccccactta cggcggcaaa agtgatactg atagctgcca
    26221 gcagcaaaat acatccgttt acggttctca ttagctcgcc accaattttt gatagtcggc
    26281 gtcactgagt gaacaatgcc agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg
    26341 gcgattgatg gcgggatcca gccaggcgtg aagtctgcgt aatcccagaa gacggttgat
    26401 tcccggcggg gcatccagcc agccaaaggg caggccactt aaaaataaga tgcgttgttc
    26461 cgcaaccgct ttcacgccct gccagagagg atcacgacga ataaaatctg cagtaacggc
    26521 ctcctgaacc agaataatat ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt
    26581 cagaccgtgg cgatcggcta tttgcgcgac gttatgcaaa cctaataatt ccgccgcctc
    26641 ggtatgcaac gaaccctgta agcccgtttc cagccctcga ggaccgcgcg cagcataaaa
    26701 gctgaggtta gcggcgggtg aggtggcgaa tgcctgagct tcaccaacga agcggctggc
    26761 gagattggct tgttctgcgg cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa
    26821 ctgttctgct gattgttcca gtttcccgtt aagcaataac cagggtattt gtgtctgttc
    26881 gctaacctgc cgtgcctggg agatccaggt ttcatccgta ttgccgcaat caacgaccaa
    26941 atcgggatgt aacgccataa gcccttccag cgagagtgtg ctggcgcgcc cggcgagtcg
    27001 tcccagcctg gggaactggc gaatgtgctc tggcaatgga attaatgcct gacgggcaaa
    27061 atcaaaagag gaaaaaccga ccattttttc tggcgcgaca gccagcaata gtaaatcggc
    27121 gggcgcgcct gcgctgacga tgcgtgtaat gttttgggct tcgggaagcg tccgtgaggc
    27181 ccagaatttg actgattgtg caaaggacgg cagatgatgc aggaacaatg tcgacgcaag
    27241 aatctgtgta aacctccgtc tggttagtga cataagtaat atcctgataa ttaaaaccgc
    27301 tatatactta cacatatagc gaaaaagatc atgtttgatg tcaaaatttg tctaaagaga
    27361 cagaatgatt tgcttgttgc ttaaataaca ctttgttcca caagaaaatt gtcagggaga
    27421 atggggagtg ggggaaaata taaatagagg tgtaatgatt aacaagttga taaaataaaa
    27481 ttaaactctt ctctaataaa agaaaataaa ggcatagacc gtagaaagag tggtatttgg
    27541 tatttcgtga tgaagaaaga gcaatgccaa ttttatgcac gtaatggcat tgctcgatat
    27601 acagtaaagt taaggcgtag gttccgcctc tttttgtttt agcggctgtg ttgttgattc
    27661 actgagtgga cgcgtcgccg gaacattgtc acacggttgc tctttcgggc agatcaaatc
    27721 cagcattttc agcgtcacgc cattggtcca gccaaagcca tcctgtaatg gatattcgcc
    27781 accgccgccc cctgttccgg tggtgctgac atcatatttt tccaccagct ttttctcccg
    27841 gtcataggtg tgctgaacat tggtcaggaa gtgccagcta atgtccatcg ctacctcttt
    27901 ttgcccgtag ttttgtaatc cttctgtcgc gacccactgc aacggtgccc agccgtttgg
    27961 cgcatcccat tgttgcccac ttttcaccga cgtggtgttc aggccgcctg gttgcagcag
    28021 atgtgttttc gtcgccgtcg ccattttgct ggcgcgatct ttcgctgccg cattgacgta
    28081 cagtgggaac agggcggccg cggttaactg attgcgcact ttatgacttt tcaggtcgta
    28141 atcggcatac cagccttgtt gatcgttcca caggtatttt tcgatccctt tttgacgggc
    28201 atttgccagc gtttcgtact ggtttgccat cgcgttatct ccggcagctt tgctggcgcg
    28261 ggagaggatt ttttccattt tgaacatcaa gctgttcaga tcgaccggta cgatgctggt
    28321 ggtgcgtaag gtgtttaact gctgcggatt gtccatccag cgcgagctga aatcccagcc
    28381 agacgcagcg gcagagcaca ggtcgcggta aatttcagtg gcaggtcgat tcggattgct
    28441 tttggcagtg gcaatatctt ccacccatga ctctggtcgt ggcgtatcgc gatcgtccca
    28501 gtagcggttg agaagggtac catcctgaag ttttacaacg cgtttttcct gttgtccggc
    28561 ttgcaggttt tcaacaccgt ccatccagta agcatattct ttttgcattt gcggcaggta
    28621 ttgtttcaac gcggcgtcgc cttcatgctg cgccagtaac tctaccatca gggcaaagaa
    28681 gggcggctgc gagcggctta aatagtaact gcggttgccg ttgggaatat gaccgtaagt
    28741 gtctatttca tgagcaaaat tggccaccat atccgcgact ttatcccagt gaccgctttc
    28801 ggcaagtcct aacatggtga agtaactgtc ccagtaatat acctcgcgaa aacgtccgcc
    28861 tggcacgaca taaggttttg gcagcggtaa tagagaatcc catttttcgg tgttttcggt
    28921 agaacgcgtt aataccggcc aaagtccgtc aatatgttcg cgcagtgatt gcccctctgg
    28981 cggaacatat ttctcgccct ctttcggcag ggtgaaattg acgttaacga aatggtgtag
    29041 atcaaatccg ctctggtttt gctgcatccg ataatcagca aggatcatca gcggatcgct
    29101 gttcggcacg gcatcggcaa aggttttttg gtccggaaaa agtttggcgt tttgcacatc
    29161 attaaacagc ggccctaata aaatatcagg cggttgtggt gttaccgatg tttcttctgc
    29221 ctgcaccgat agcgcagcga aacacaaaaa gatgcaggct ggaattaacg ccattttttg
    29281 cgggcgagaa ggggcggggg atttcatcaa tcattctcct ttggcgaaac cgaataaaac
    29341 ggtattcaag tatcagaaaa ccttagttca ggatcgcact agctgcatga tcgaaatcac
    29401 attctgcgaa ctgacagaca ttttagaaaa gggaattaaa gggataaata acggcgaaat
    29461 agagtgataa aataacatct gtttattagt cagccagcga cgttgggttt gcgattagcg
    29521 aagaaaaatc atcataattt ggtgaaaaat ataacaggta gttaaaaaca tcagtgcaga
    29581 gtaaattgcc ggatgacatc agaatgatgc catccgaacg gtggtttaac cctgacggtt
    29641 gaaacgttgc gttttaacgt ccagcgttag cgtttcttct ggttgtatcg catacagttt
    29701 ctcaccctgc tggcaaatcc agccaattcc cagttcacgg gcgattaggg cactgtggga
    29761 tagcggactt ccggcgctaa ggcagatacc ttttacaacc gctggatcca gttgcagtac
    29821 tgtggaggga taaatgttct ccgccagtag aatagttggc gagttaaact gcgggagttc
    29881 ttctttcgtt tgggtcaggt ggaccagggt gcgatgcaga agatcgtcca catcgatata
    29941 gcgagcctgt agatattcat catccagctg ttggtattgc tggctaagtt ctttaagaac
    30001 ttgctgccag gcatattctg ccgtgcaatg ttcatgctga aggagttcgc ttgccgccgc
    30061 ctgcagttcc ggatcatcta acagtgtatg gtgaccagaa aagattgcgg caatatcgtc
    30121 aagcccactg gtttctgctt tcgctgttaa cgtcatcaga tctaataacg tgaagtcaat
    30181 ggcctggcgt aatcgttctt gttcttcttc cacggtcagg gttgattttg cctgtaccgt
    30241 acataaaaca ggttgataat aaaaggcttt acccgaaaca ggcggaacgg gacgcagagt
    30301 aggtggagca acttcctccg tttcaccaaa gttatcttca gccagctgac ggaaagcgat
    30361 cagtgcctct tcagcttctg gtcctttcgc aatcaggcgc agcgtatcgt tatagcgaac
    30421 ttgtagtaac gcaatctggt taatactctc cggtgtaacg catttgccgt ttttctccag
    30481 caacatatcg gcattaaatg tcgataaggt ataaaccagc cgggaggccg gacgtacatg
    30541 caggccgtta cggtttttta tgaccaccga cagagaacgg gcttcttcat cgtacggagg
    30601 acatgtgtca gagatttcag tgtcggagga cggtaaaccc agttgttcac gtttggcttc
    30661 cagcgcatgc atggcgtcaa agatcacttt gtcgatatcc gcccccgagg ccgcgctgac
    30721 cgttgctgcc agtgtacctt cgaccaacgg cgcagcacac aaacgtactt ttgcggcgat
    30781 ctcgggagcc agcaattcca gcgcagtttc agcactcaat aatgcgctac ccatatccat
    30841 catgaccagc acatggtcgg catcggcaac agattcgatg gcctccatca ctttgacggc
    30901 atcggtacca atgggatttt gtggatcgtc aattcccgcg gcaatggcga ttttacaatt
    30961 atcactcatt aacatctgac gggctaattc accgacacct tctcccagtc ggctgctatg
    31021 tgaaactatg accaggttta ccatcaccaa ttccttactc ttttgcggct aacgccaaca
    31081 tttgcatcat aaacatcacc gaggtcgcgc cgggatcctg gtgaccaata ctgcgttcac
    31141 cgagataact ggcgcggcct ttgcgggctt gcatcgtaat cgtactttgt gcagcggatt
    31201 cggcgatgct actggcagcg tcgagcgccg ccggaacagg gagattttgc tcgcaggact
    31261 gacgtaacga ttccaccacc ggcacccaca catcacacat ggttttatcg ccaggttcgg
    31321 ctttcccgcg actgattacg ccgtccgcgc catcgcggaa catctgataa agctcttcca
    31381 gcgtcaggct ttgccgtgcc tgggttgtct gtgcggcgcg gataaagaag gtaccgaaca
    31441 gcggaccact ggcacctccg acgctggaaa gtagcgtcat gccggtattc ttgagaatga
    31501 aaccgatatc tttatctgcg atagcaggga gtttttccac cactttgcta aagcctcgat
    31561 tcatatttag cccgtggtca gcgtcgccaa tctcgcgatc cagtccagta agatactcgc
    31621 tctcggtgct gaaaatatcg ccacaacgag tgagccagtt aacaatttga gttctgctca
    31681 gtgacattgc tttctcctta tttaccccag ttaagggccg gggtgtggac aggggcgtcc
    31741 cagagtgcca gcgtttcgtc atcaactttc agtaaggtga ttgagaaacc ggtcatatcc
    31801 agtgaggtgc agtacgcgcc aattaaatta cgttcgatag tcaatcccgc ttgctggcaa
    31861 cgtgtggtca ggcggttata gacgccgtac agctcagaaa gcggagttgc gccaagattg
    31921 ttaaccagcg caatcacccg atcgccagac tggagcggtt gtttggtttg tggttcttcc
    31981 tgccaactgc cttgttgata atcccagaaa cgcaaagtgc gatggtatga gccattttcc
    32041 agcagggtgt cgaacatttc atcgacggtt tgatcaaggg aagagaaggg gcggcggtca
    32101 atacctggct caccatgaat gccgacgcca aactccatct cattatccgc cagggtaaaa
    32161 gaaggtttgc ccgcggcagg aacggtacag gcaccgagag cgatacctat tgaatggcct
    32221 tgattattca acttacgccc cagttccgca caggcgtcca gtgagccgcc acgctccgcc
    32281 gctgcaccta cgagtttttc aattaatacg gtgttggcaa cgccgcgccg cccggcggta
    32341 taaagactgt cttttactgc aacgtcgtca tcaatgacca cagtggtcac ttttacgccg
    32401 ctatcgtgca gtaactcggt tgctgtttcg aagttaagaa tatcgccggt gtaatttttg
    32461 ataatcaaca gtacaccttc gccgccatca atttgcatgg cgcattcaaa gattttatcg
    32521 ggcgtcggtg aggtgaaaat ttcgcccgga caggccccag aaagcatccc ctgaccgata
    32581 tagccacagt gcatcggctc gtgtccgctg ccgccacccg acagcagggc gactttccct
    32641 gcaacggggg catcagctcg ggtgacatac accggatcct gatgcagtgt cagcgatgga
    32701 tgcgccttcg ctagtcctgc cagttgttcg tccagtacgt cttgcacatc attgatcaat
    32761 tttttcatta ttttgctcca gcaattacgg tagggcatgg atgatgttca acgacacggc
    32821 gaccactgac tgccgatgaa tccattgtgc atcaggcaag ggaaaagaaa attagcccaa
    32881 attaggtttc atagtgaaac atatgcttta atgaatgttc catattgaaa cttttacgtg
    32941 tattaatact taaaattgcg agccggaaca ccttttgtca taagggatgc gggatatgag
    33001 tggcgctttt aacaacgatg gtcggggcat atctccctta attgcaacct cctgggagag
    33061 atgcaataag ctgatgaaac gggagacatg gaacgtacca caccaggccc agggcgtgac
    33121 atttgcttct atttatcggc gtaagaaagc gatgctgacg ctcgggcagg ctgcgctgga
    33181 agatgcctgg gaatatatgg caccgcgaga gtgtgcgttg tttatcctcg atgaaaccgc
    33241 ctgtattctc agccgtaatg gcgatccgca aaccttgcag caactaagta cactgggatt
    33301 caatgacggc acgtattgcg ccgagggaat tattggtact tgtgcgctat cgttagcggc
    33361 tatctctggt caggccgtga aaacgatggc cgatcaacat ttcaaacagg cactctggaa
    33421 ctgggccttt tgcgcaacgc cgttgtttga cagcaagggc cgattgacgg gaacaatagc
    33481 gctggcgtgt ccggttgagc aaactaccgc agctgatttg ccgttgacgt tggcaatcgc
    33541 acgcgaggtc ggaaatttac tgctgacgga cagtttgctc gctgaaacta accgtcattt
    33601 aaatcaactt aatgccctgt tagaaagtat ggatgatggc gtgattagct gggacgagca
    33661 gggtaatttg caatttatca atgcccaggc ggcgcgggtc ttgcgccttg acgcgacggc
    33721 aagtcaggga cgagcaatca ctgaactctt aacgttaccc gccgtattgc aacaagcaat
    33781 aaaacagtca catccgctca aacacgtaga agccaccttt gaaagtcagc atcagtttat
    33841 tgatgcggtg ataaccctta aaccgataat agaaacgcag ggaaccagct ttattttatt
    33901 gctccatcct gtggaacaga tgcggcagtt aatgaccagt caattaggaa aagtcagcca
    33961 taccttcgct catatgccac aggacgatcc gcaaacccgc cgcttgattc attttggtcg
    34021 ccaggcggca cgcagtagct ttcctgtcct gctttgtgga gaagagggcg tgggcaaggc
    34081 actgctaagt caggcaattc ataatgaaag cgagcgtgca gcggggcctt atatcgccgt
    34141 caattgtgag ttatatggtg atgcggcgct ggcggaagaa tttattggtg gcgatcgcac
    34201 ggatagtgaa aatggccgtc tgagtcggct ggaactggcg cacggcggca cgctgtttct
    34261 tgaaaagatt gaatatctgg cggtggagtt acagtctgcc ttgcttcagg ttatcaagca
    34321 gggggttatc acgcgactgg atgcgcggcg tttaataccc attgatgtca aagtgattgc
    34381 cacaacgacc gcggacctcg caatgctggt ggaacaaaat cgttttagtc gccagctgta
    34441 ttacgcgctg catgcatttg aaattaccat cccgccactg cgtatgcggc gtggcagcat
    34501 tccggcgctg gtgaataaca aattacgcag ccttgaaaaa cgcttctcta cgcggctgaa
    34561 aattgatgac gatgccctcg ctcgcctggt ttcctgtgca tggcctggca acgattttga
    34621 actttacagc gtcatcgaga atcttgctct gagtagtgat aatgggcgca ttcgcgtcag
    34681 tgatttgccg gaacatctgt ttaccgagca ggcgacagat gatgtcagcg ccacccgcct
    34741 ttccaccagt ctgtcatttg cggaagttga aaaagaggca attattaacg cagcccaggt
    34801 cacaggcggt cgcattcagg aaatgtcggc tttacttgga atcggccgca ccacgctgtg
    34861 gcggaaaatg aagcaacatg gcattgatgt gagtcagttt aagcgccgtg gatagcggga
    34921 gagtatgcac tctcccgcgt atgacttgct aattaatagc gaccatgact gccatgattt
    34981 ggcccaccat ttgaaggtgg cattggctta ccggaagccg caggaccacg cgggccatcg
    35041 ttgtggcggt gatgatggtg aggaacacaa gcacttaaca ggctcgcgaa gacaattgct
    35101 aacaggctga agataaatgt ttttttcatg acaaactctt gaacggttaa tgcactgtga
    35161 ttgttatcac ataaaagccg cattgttaat attgccagga gtctgattat gactttcggt
    35221 aactcagaat tgaaagaagc agcgtagcag gcgtaaaaaa gccattatgt gcgactcaca
    35281 taatggctcg ttattaaccg ccaggatatt acgcggaatt agacgttgaa caggaagttc
    35341 atcacatcgc catctttaac gatgtaatct ttgccttctg cacgcatttt gcctgcttct
    35401 ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa acgagatggt ttgtgcacgg
    35461 ataaagcctt tttcaaaatc agtatggatt ttgcccgctg cctgcggcgc ggttgcacca
    35521 accggaatgg tccatgcacg cacttctttc accccagcgg tgaagtaagt ttgcaggttc
    35581 agcagcttat aaccggcacg gatcacacgg ttcaggcccg gctcttccag cccaagctcc
    35641 tgcataaact cgtcacgctc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
    35701 caaaccggaa ctacaacaga accttctttc gctgcgattt cacgcacctg gtcaagatac
    35761 gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg ttttagcgtc
    35821 aggaagctca ggtaacgaac agccgctttc tcttcagcgc ttaaatccag cgcacgcagc
    35881 atacctgcgt tttccagctg gggcaggcat ttttccagta ccgccagctc ggctttcgcg
    35941 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc acaggtgtcg
    36001 agatccgcca gcgccagttc ggtgttgata acttcaatat catccgccgg gttaactttg
    36061 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttcg
    36121 gtttcacgga tgttggtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
    36181 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gtgtacgctg cggtttcacg
    36241 atttcagcca gttgatccag gcgagggtca ggcattggta cgacgccagt gttcggctcg
    36301 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
    36361 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat gatttaaatc
    36421 accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat ggaaataact
    36481 ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa
    36541 aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt tcagtacaac
    36601 gcgccgcttc gtcgatggct tcatcaatta acttctgttc actaacaggc ggtttgccta
    36661 acacaaaacc gacaacttta tttttatcgc ccggatgacc gattccgatg cgtaaacggt
    36721 gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg tgaccaccat
    36781 gaccgccacc caacttaaat ttggcgacgc caggaggcag atccagttcg tcgtgggcca
    36841 ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg gctttgccgc
    36901 tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca agggtgactc
    36961 gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaat cgctctgcca
    37021 gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca gcaccggggt
    37081 tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg tcctgagtgt gtacataact
    37141 ggcgcgtagt ttactggttg cggccccgct tgacaaaaaa ctgcgtatca aatgcagata
    37201 atgcaattat tgcctgagtg gactattaga aagtcaaggt gttcaggcgt ttatttgtaa
    37261 agttttgttg aaataagggt tgtaattgtg atcacacccg cacataacct acagggtgtt
    37321 gtctatactt tacacataag gaagaggggt attccctgtt acaacccaga aagttccgga
    37381 ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa cgtgctcatg gggttgggtc
    37441 tggtggtaat ggtggtcggc gtggggtatt caatcctcaa ccagttacca cagtttaata
    37501 tgccccagta tttcgcacat ggtgcagtgc taagtatttt cgtcggtgcg attctctggc
    37561 tggcaggtgc ccgtgttggc gggcatgaac aggtgtgcga ccgttactgg tgggttcgcc
    37621 actatgacaa acgttgccgc cgtagcgata atcgccgtca tagctaacaa ataatgcagt
    37681 ttgctgacca gtcagtttgc gctgactggt caatctcgta cttataaatc cgccatcgcc
    37741 gcgcgacgat tcgggaagaa tgcgaggcgt cccgggatcg gttgaatgcc tgcgcgagcc
    37801 atagtgcgca gtggctggaa ttccaggttg cacacgcgca gttcacatcc ttcgggcaga
    37861 cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat caagtaccgg aacggcatcc
    37921 cacttcagaa tcacaatccg tttgccttca agacgtgact ccaggtccgt gaataagcct
    37981 tctgcggcag caaaaaacag cgggccaata acgcgcagaa ccaggacatc gtctggaacg
    38041 tctacgacta ccggtgccag gcgagtcata cgtgcgatac gacgcataaa cagcagcgat
    38101 gccagcacga tccccacgct gatggcaata accatatcga acagcacggt cagcgacatg
    38161 cacagcagca tgacgatgat gtcatctttc ggtgcatgac gcagcaagtc gaccactttg
    38221 tgcgcttcac tcatgttcca cgccaccatc aacagcaggg ctgccatggc ggaaagcggc
    38281 agccaggaga gcagcggtgc cagtaccagc agggcaagaa taaccagaat agagtggatc
    38341 accgccgaga taggggaagt tgccccggca cggacgttag cggcagaacg cgcgatggca
    38401 gctgtagcgg taataccacc aaagaacgga gcgataatat tccccagtcc ctgtccaacc
    38461 agttcgctgt tcgccttgtg tttcgtcccg gtcataccat ccagcaccac ggcgcagagc
    38521 agagattcga ttgcgccgag cattgccatt gagaatgccg caggcagcag ggtgcgaata
    38581 gaatcccagg ttagcgtgaa ttctgaatta ggcagatccc acggcagcac cagttgcggc
    38641 agcagttgcg gaataccgtt accctgagaa ccatcggcca ggacgtagtg gaattgcgaa
    38701 ccgatggtag caacatgtcc gccgagcagg ttaacaatcc ccatcaccgc gcaaccagcc
    38761 agcaaggccg gaaggtggcc gggtaaacgt atgcccagac gcggccagaa aaccagaatg
    38821 ccaagcgtca caatgccaat ggctgcatca cccacattaa tggtcggcag cgccataaat
    38881 aatgcgccga ctttttgtag ataatgttcc gggacatggg ccatttgcag accgagaaaa
    38941 tctttaatct gcatggtacc gatggtgatc ccgatacccg aggtgaaacc taaggtgacg
    39001 gaaaccggaa tatactcaat caggcgccca aagcgtgcca gccccatcag aatcaaaaag
    39061 atccccgaca gcaaggtcgc aaccagcagt cctgccagtc caaactgttg cgaaacggga
    39121 tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc taaagcgtga cccccccgtc
    39181 agagcaatga caatccccgc aacagctgcg gtatataaac cgtactgggg ggccacacca
    39241 ctaccaatag ccaacgccat cgccagcggg atagcaataa tcccgacggt tatcccggca
    39301 atcaggtcac gggtaaaccg tgcggcagta tatttttctt tccagcaagc gtcgatcaga
    39361 gcgcggaaag gcatcacatg tgaggaaaat attttgttca caataatgtt tcatccgtga
    39421 gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat aggtcataca aatggatatt
    39481 acagacaaaa aaacccgccg cagcgggtct ttgagccggg ttcgattagt gttcgaacat
    39541 ggcagagatc gattcttcgt tgctgatacg acgaatcgct tcggccagca tacctgacag
    39601 ggtcagagta cgcacgttcg gcagtgattt gatttcatcg ctcagcggaa tggtatcgca
    39661 gacaacgact tcatcaatta cagagttacg caggttgttc gccgcgttgc cagagaagat
    39721 cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt tctttcagag cttcagcagc
    39781 tttacacagc gtaccgccag tgtcgatcat atcatcgacc agtacgcagt cacgacctgc
    39841 aacgtcaccg atgatatgca tcacctggga aacgttcgca cgcggacgac gtttgtcgat
    39901 gattgccata tcggtatcgt tcagcagctt agcgatagcg cgggcacgca caacgccgcc
    39961 gatgtccgga gaaaccacaa ttgggttatc cagattcagc tgcagcatgt cttccagcag
    40021 gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc
    40081 gtgcagatcc actgtcagca cacggtcaac accgacgctg gagaggaagt ctgcaaccac
    40141 tttcgcagtg attggtacac gagcggaacg gacgcgacgg tcctggcgcg catagccaaa
    40201 gtaggggata acagcggtga tacgacctgc ggaagcacga cgcagggcat caaccataac
    40261 gactaattcc atcaggttgt cgttagtagg ggcacaagtg gactggatga tgaaaatatc
    40321 accaccgcgt acattttcat taatttgtac actgacttcg ccgtcgctaa agcgacctac
    40381 agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata cgttgtgcta gttccggggt
    40441 ggcgttacca gcaaaaagct tcatatcagg cacgagaaga acctcaggca tgcgtccatt
    40501 ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg attctttcca ggcggtgtat
    40561 taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt caccgaaact cagcttgccc
    40621 ggcttaaagc atggctctgt gcaatgggga aagattaacg cctttcgcca caaagccatt
    40681 gagccattcc ggggcttgct ctagcacctg gcgggcttca gactctgtat caaattcagc
    40741 aaagacacag gcccctgtcc cagtcaggcg cgacggggcg tattctaaca gccaggaaag
    40801 caccgcatca acctcgcgaa aacgttttct tgcgataacc tcgcaatcat tgctgaattc
    40861 acattttagc aacgtttcta ttgacctttt tggcgtattg cgcgggagtt caggatcttt
    40921 aaaaatcacc ggcgtcggaa tactcacgcc agggtgtgcg accagatacc atttctctgg
    40981 cgggtctacc ggcgttaata tttcgccaac accttcggca aaggcggcat gaccacggac
    41041 aaagacagga acatctgcgc ccagcgtcag ccccatttcc gccagctcat ccatgcttag
    41101 tccgcattgc cagagatgat ttaacgccac cagaaccgtc gcggcattgg atgaaccacc
    41161 gccgagaccg ccgcccatcg gcaaacgctt gtcaatgctg atattcgcac cgcttcccgt
    41221 cgaaagacgc ccgctgtctg ccgcagtttt catcaacaac ctcgctgcgc gaacgatcag
    41281 gttatcttca tgttccacgc cttcaacggg cgttaacaga cgaatatccc catcgtcacg
    41341 aagctcaatg ctgatggtgt cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg
    41401 gtaaccatcc gcacgctgac cggtaatgta taaaaacaga ttaagttttg ccggagaggg
    41461 ccactgtgtc cgcattattt cactatccag ttatccattt ttaacttgat gcgttgacca
    41521 ccgtcggtga gttccatatt ggctggcatc gcaggttgag tttttgtgtc ataaccaccg
    41581 taaacaactt tccagttttt gccattctgg ctgtaggtga tttcactcag gcggtactgg
    41641 tcgtcaagtt tgtagtcagt cgcatcaccc ggtaaaccta aaatccactg gcgcaagctg
    41701 ttgagcggaa ttggcattcc ggtcaatttg ccaatcatct cttcggcgtc atcggaggta
    41761 taacgctgac ctttattgtc gactaactgc acgttacccg gttgagcatt cagctccagt
    41821 tccgtgctgc ccaatgggtt agtcagcagc agacggtagc gatcctggcc agtttgttgc
    41881 cagaagaaac gggcgtacac tttttgttgg tcagaaatat aagcgaacgc gccgcgagtt
    41941 tgatactgat taagattgcg cacgtcttgc tgatgctgac gccattgtgg cgaatccggg
    42001 cttttgccag gacctttggg cgtggtaacg gaacaggcag tgagcacaag agcagccagc
    42061 ggtagcaggc ggataagacg aaaatcgggc aggggcataa taatgacaag tccttgagat
    42121 acgttgcagt tataaccctt aatgctagcg ttaccgttag ccatcgtcta tgttcacgtt
    42181 gtcttaattg ccagaatcta acggctttcg gcaattactc caaaaggggg cgctctcttt
    42241 tattgatctt acgcatcctg tatgatgcaa gcagactaac cctatcaacg ttggtattat
    42301 ttcccgcaga catgaccctt ttagcactcg gtatcaacca taaaacggca cctgtatcgc
    42361 tgcgagaacg tgtatcgttt tcgccggata agctcgatca ggcgcttgac agcctgcttg
    42421 cgcagccgat ggtgcagggc ggtgtggtgc tgtcgacgtg taaccgcaca gaactttatc
    42481 tcagcgttga agaacgggat gacctgcaag aggcgctcat tcgctggttg tgtgattacc
    42541 ataatctcaa cgaagacgat ctgcgtaata gcctctactg gcatcaggat aacgatgcgg
    42601 tcagtcattt aatgcgcgtt gccagtgggc ttgattctct cgtccttggc gagccgcaga
    42661 tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa aggtcatatg aaggccagcg
    42721 aactggaacg catgtttcag aaatctttct ctgtggcgaa acgcgttcgc actgaaacag
    42781 atatcggtgc cagtgctgtg tctgtcgctt ttgcggcttg tacgctggcg cggcagatct
    42841 ttgaatcgct ctctacggtg acggtactgc tggttggcgc gggcgaaacc atcgagctgg
    42901 tggcacgtca tctgcgcgaa cacaaagtac agaagatgat tatcgccaat cgcactcgcg
    42961 aacgtgccca aattctggca gatgaggttg gcgcggaagt gattgccctg agtgatatcg
    43021 acgaacgtct gcgcgaagcc gatatcatca tcagttccac cgccagcccg ttgccgatta
    43081 tcggtaaagg catggtggag cgcgcattaa aaagccgtcg caatcaacca atgctgctgg
    43141 tggatattgc cgttccgcgc gatgttgagc cggaagttgg taaactggcg aatgcttatc
    43201 tttatagcgt tgacgatctg caaagcatta tttcgcacaa cctggcgcaa cggaaagctg
    43261 ctgctgtgga ggcggaaact atcgtcgccc aggaggccag tgaatttatg gcatggcttc
    43321 gagcacaaag cgccagcgaa actattcgcg attatcgtag ccaggcagag caggttcgtg
    43381 atgagttaac tgctaaagcg ttagcggccc ttgaacaggg cggcgatgcg caaaccatta
    43441 tgcaggatct ggcatggaaa ctgactaacc gcctgatcca tgcaccaaca aaatcacttc
    43501 aacaggccgc ccgtgacggg gataacgaac gcctgaatat tctgcgcgac agcctcgggc
    43561 tggagtagca gtacatcatt ttcttttttt acagggtgca tttacgccta tgaagccttc
    43621 tatcgttgcc aaactggaag ccctgcatga acgccatgaa gaagttcagg cgctgctggg
    43681 tgatgcgcaa accatcgctg accaggaacg ttttcgcgca ttatctcgtg aatatgcgca
    43741 gttaagtgat gtttcgcgct gttttaccga ctggcaacag gttcaggaag atatcgaaac
    43801 cgcacagatg atgctcgacg atcctgaaat gcgtgagatg gcgcaggatg aactgcgcga
    43861 agctaaagaa aaaagcgagc aactggaaca gcaattacag gttctgttac taccaaaaga
    43921 tcctgatgac gaacgtaacg ccttcctcga agtccgtgcc ggaaccggcg gcgacgaagc
    43981 ggcgctgttc gctggcgatc tgttccgtat gtacagccgt tacgccgaag cccgccgctg
    44041 gcgtgtagaa atcatgagcg ccagcgaagg tgaacatggt ggttataaag agatcatcgc
    44101 caaaattagc ggtgatggtg tatatggtcg tttgaaattc gaatctggcg gtcatcgcgt
    44161 gcagcgtgtt cctgctacgg aatcgcaggg tcgtattcat acttctgctt gtaccgttgc
    44221 ggtaatgcca gaactgcctg atgcagaact gccggacatc aacccagcag atttacgcat
    44281 tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt aacaccaccg attcggcaat
    44341 tcgtattact cacttgccga ccgggattgt tgttgaatgt caggacgaac gttcacaaca
    44401 taaaaacaaa gctaaagcac tttctgtact cggtgctcgc atccacgctg ctgaaatggc
    44461 aaaacggcaa caggccgaag cgtctacccg tcgtaacctg ctggggagtg gcgatcgcag
    44521 cgaccgtaac cgtacttaca acttcccgca ggggcgcgtt accgatcacc gcatcaacct
    44581 gacgctctac cgcctggatg aagtgatgga aggtaagctg gatatgctga ttgaaccgat
    44641 tatccaggaa catcaggccg accaactggc ggcgttgtcc gagcaggaat aatggaatat
    44701 caacactggt tacgtgaagc aataagccaa cttcaggcga gcgaaagccc gcggcgtgat
    44761 gctgaaatcc tgctggagca tgttaccggc agagggcgta cttttattct cgcctttggt
    44821 gaaacgcagc tgactgacga acaatgtcag caacttgatg cgctactgac gcgtcgtcgc
    44881 gatggtgaac ccattgctca tttaaccggg gtgcgagaat tctggtcgtt gccgttattt
    44941 gtttcgccag cgaccttaat tccgcgcccg gatacggagt gtctggtgga gcaggcactg
    45001 gcgcggttgc ctgaacaacc ttgccgtatt ctcgatctcg ggacgggtac cggggcgatt
    45061 gcgctggcgc tggccagcga gcgtccggac tgcgaaatta ccgctgtaga tcgtatgcca
    45121 gatgctgtct ctctggcgca acgtaatgcc cagcatctgg ctatcaaaaa tatccacatt
    45181 ctgcaaagcg actggtttag cgcgctagcc gggcagcagt ttgcgatgat tgtcagcaat
    45241 ccgccgtata ttgacgagca ggacccacat cttcaacaag gcgatgtccg ctttgagccg
    45301 ctcactgcgc tggttgcggc agacagtgga atggcagaca tcgtgcatat catcgaacag
    45361 tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg agcatggctg gcagcagggt
    45421 gaagcggtgc gacaggcatt tatcttcgcg gggtatcatg acgtcgaaac ctgccgtgac
    45481 tatggggata acgagcgcgt aacgctcggc cgctattatc aatgacaagt ttttctacgt
    45541 tgcttagcgt tcatctcatt agtatcgcgc tttctgttgg gctattgacc ttacgtttct
    45601 ggctacgtta tcagaagcat ccacaggcat ttgctcgctg gacgcgcatt gtgccgccgg
    45661 ttgtcgatac gctgttattg ttaagcggca ttgcgttgat ggctaaagcg cacatccagc
    45721 cattttccgg gcaggcacag tggctgactg aaaagctgtt tggagttatc atttatattg
    45781 ttttgggttt tattgcactc gattatcgtc gtatgcacag tcagcaggcg cgcattattg
    45841 ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa actcgccacc acaaaagtac
    45901 cgttactggg gtaagtcatg agatcgttag ctgatttcga atttaataaa gcgccattgt
    45961 gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga ttttccctcg caagatgttt
    46021 acgacgaact ggagcgtctc gttagtctgg cgaaggaaga aattagccag cttctgccat
    46081 tagaagagca gctagaaaaa ttaatcgcgc tgttttacgg cgaatggggg tttaaagcct
    46141 cacgcggcgt ttatcgtctt tccgatgcat tatggctgga ccaggtgtta aagaatcgac
    46201 agggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt ctcgatttgc
    46261 cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt ccggatggcg
    46321 aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg ctggatgtgt
    46381 ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt gatgaagctg
    46441 ataacattga ggtaatccgc aaattgctgg atacactcaa agcttcgttg atggaagaaa
    46501 atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac ccggaagatc
    46561 cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa cacgttgcgt
    46621 tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc gaaatgatcc
    46681 gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta atcgacattt
    46741 tactcaagat taaggcgatc ctatgaaaca aaaagtggtt agcattggcg acatcaacgt
    46801 agcaaatgac ctgccgttcg tactgtttgg cggtatgaac gtgttggaat ctcgcgatct
    46861 ggcgatgcgc atttgcgagc actacgtaac cgtgacccag aaactgggta tcccttacgt
    46921 gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc ggggaccggg
    46981 cctggaagaa gggatgaaaa tcttccagga gttgaagcag acttttggcg tgaaaattat
    47041 caccgacgtt cacgaaccaa gccaggcaca gcccgttgct gatgttgtag atgtgattca
    47101 gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga aaaccggtgc
    47161 ggtaattaac gtcaagaaac cgcagtttgt cagcccggga cagatgggta atatcgttga
    47221 taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgcggtg ctaacttcgg
    47281 ctatgacaac ctggttgttg atatgctggg cttcagcatc atgaagaaag tgtctggtaa
    47341 ttcgccggtg attttcgacg ttacccacgc actgcaatgt cgcgatccat ttggcgcagc
    47401 ttccggcggt cgccgtgctc aggtggctga gctggcaaga gccggtatgg cggtaggtct
    47461 ggcggggctg tttattgaag cgcatccgga tccggaacat gcgaaatgtg atggtccatc
    47521 cgcgctgccg ctggctaaac tggaaccgtt cctcaagcaa atgaaagcga ttgatgatct
    47581 ggtgaaaggt ttcgaagaac tggataccag caagtaatct ttttgtttga aaaataaagt
    47641 cttagcgttc tgcgttaaga ctttttttat gggtgccgga tacaaaaaag gccgcaggct
    47701 gttacccctg cggccggttt cgggcgcata ttgccatcac ggcagcctga cgcccgtttt
    47761 caccttactt ccggttacgc caccagccga caatcgctgc ggtaataatt cccgccagga
    47821 tcggtgctgc caggtcgtgc cagaaaatca tggcaaactg cgcgagcgtc atatagccgc
    47881 cttgttgtaa tgacaacatt ttgcggctat tcttgaattg ttgatggttc aagattagcc
    47941 cccgttctgt tgtcaggttt tacctctcaa cgtgcggggg ttttctcttt ccagcaacca
    48001 atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc cggcttggtg
    48061 tggatactac gtcgcaattc atcttcactt catccctgaa atgtttgcat tgaagagtac
    48121 attccggctt ttcaacagct gttgcagtgg tttcgtgcag gttgtggtac aggctcgcaa
    48181 ttctgattaa cgacctgtcc aggtagtatg aagaaataca ataagcagga atttatatta
    48241 tctcccccga tttccgtcat cggaggagat ataactgtca ggcaaatatc gtcatcaaat
    48301 aggcggcaaa cagtgccaga tgcgctgcgc cattgagcac gttagtacgt ccggtggaga
    48361 aggagatatg gcacagcact aaagaggcca ccatcaccac catttctggc gcaccaagtg
    48421 caaactgcaa ttcgttaccc gtcataaagg caattagcgt gacgacaggt acggtaagcg
    48481 aaatggttgc taacactgaa ccaaagaaca gattcatcgc gcgctggacc tggttgttca
    48541 acactgcttt taacgcacct aaaccttccg gcgacagaat caacagagcc accaggaagc
    48601 cagtaaaggc gacaggggca ttcatgctgt cgagcaatgt ctccagcggg ctggcgttca
    48661 ttttggtcac cgcaataacg gcaatcagat ggacaatcaa ccagatagca tgccacaggc
    48721 tgctatgggc agacggttta ccgtggtgcg ggtcgtcatc atcactgtca tcttcgtgct
    48781 cgtagacaaa taaactttga tgcgttttgg tctggatcag caaaaatacg ccatacattg
    48841 ccgcagaaat taatgctacc agtaacgcct gacctgttga aaaattcgcc gcaggcagag
    48901 ccattggaaa taccagtacg attatcgcca gggggaacag cgcaattaaa tactgcttga
    48961 taccaaacag attcatatat tgggtggcaa acttgcgtcc gcccaacaat aatgaaaagc
    49021 caaccagccc accggtaaca atcataatga ttgaatagag cgtatcacgc attagcgttg
    49081 gcgcggcgtc gccggttgcc attaaagctg aaatcaaact gacttcaaga ataaccactg
    49141 aaaggctaag aataagcgaa ccgtaaggtt ctcccaggcg atgggctaat acgtccgcat
    49201 gacggacaac actaaaggcg ctacttaaaa taccaataag cgcaagaaga ttgatggcaa
    49261 tgaccactgg tagtgtctgg ctgcctcccc acaggaacag cactaccagc gccagaaccg
    49321 ggaaaataag cgaagtctcc ttgtggcggg tttttaccgc ctcttgagca tttgacatta
    49381 tggttatccc tttgcagatg aatttatcga aaatgtaaaa aataggtagg aaaaataaca
    49441 gaaattgtct ggatatcggt aacattttac gaatttttac cccgctgtcg attttttact
    49501 catttgggca taaaaataag taatacgttt agacaatgtt tgttttagca cttcttaaga
    49561 agagtctgac atgaaaattc ttatgttttg gcaagagtag atattgttga ccacacttaa
    49621 tgttcaactt tgtaaaagga gtcaacgatg ccgtataaaa cgaaaagcga tctgccggaa
    49681 agcgtaaagc acgtgcttcc gtctcatgcc caggatatct ataaagaagc gttcaacagc
    49741 gcatgggatc aatataaaga taaagaagat cggcgtgatg acgccagtcg cgaagaaaca
    49801 gcgcataaag tggcctgggc tgctgtgaag catgaatatg ccaaagggga tgatgataaa
    49861 tggcataaaa aatcgtaaaa ctggtagctt agttaaagct attcgtgcgg tgttgccttg
    49921 caagtggtcc gtggattgca tattgtcccg ttagtggttt caaaatgagc agtaaaaatg
    49981 tccggaagac accaaaaagt tgtcgcaggg aagtatgcag tggcggaggt gtaaggtgat
    50041 aacgcgtgat ttcttgatga atgccgattg taaaacggca tttggtgcca ttgaagaatc
    50101 actcttatgg tcagcagaac aacgggcggc ttcgctggcg gcgacgctgg cttgtcgacc
    50161 tgatgaggga ccggtgtgga tcttcggtta tggatcgttg atgtggaatc cggcactgga
    50221 gtttaccgag tcgtgcaccg gtacactggt tggatggcat cgcgcgtttt gcctgcgcct
    50281 gaccgccggg cgcgggactg cgcaccagcc gggacgaatg cttgcactga aagagggcgg
    50341 acgcaccaca ggcgtcgcct atcgactgcc agaagagacg ctggagcagg aactaaccct
    50401 gctgtggaag cgcgagatga ttaccggctg ttatctgcca acctggtgtc agcttgatct
    50461 tgatgatgga cgcacagtaa acgccattgt gtttattatg gacccgcgac atccagaata
    50521 tgaatctgac actcgcgctc aggtcatagc gccgttgatt gcggcggcga gcggtccgct
    50581 gggaaccaat gcacaatacc tgttttcact ggaacaggag cttatcaaac tgggaatgca
    50641 ggacgatggg ctgaatgaat tgctggtatc ggtaaaaaaa ctgctggcgg agaattatcc
    50701 ggatggtgtg ttacggccgg gattcgcctg agtaaacttt cctcatagtg gggcgtcaga
    50761 cgccccctca aacattaaaa tgtgagcact ttatcggctg acagcgtcca ttgcgccagt
    50821 tcaacaagag taccgatttc caccccatca atcagaggaa gtgtactaat cccgcgcccg
    50881 tcggtacagg ttttgcacaa tttcaccggt acattctgag cggtaaggat ctccagcatt
    50941 tgctgaatgt tgtagccttc ccctggtttt tgcccgcgca acccggctgt gaccgcatca
    51001 gacatcagga acagacgcag atccagattg ttctcctgct ctcgtaacgc aatggccagc
    51061 cgcaagctgt taaacaagga ttcgctcccg taaggtgcgc cattggcaac gatcacgatt
    51121 ttttgcatta tttactcctg tattcaggga attagacact catcttctat cttactgctt
    51181 ctgcagcgtc tgaccaatcg gtcacatttt taaggatttt cctgaaagcg cgagaaaata
    51241 cgacaaaagg tgccagtaat cgttattctt taaggctatg gtttttcatt tttaccggaa
    51301 gttaccgacg ttttgagccg tttcgttcct cgcattattc cgttttattt actcttgctg
    51361 gcggcaggcg gtacagctaa cgcacaatct accttcgagc aaaaagcggc aaatcccttt
    51421 gataataaca atgatggtct gccggattta ggcatggcac ctgaaaatca tgatggggaa
    51481 aaacactttg ctgaaattgt gaaagatttc ggcgaaacca gtatgaatga taacgggctg
    51541 gatactggcg agcaggcaaa agctttcgca ttgggaaagg tccgcgacgc gcttagtcaa
    51601 caggttaatc agcacgtaga gtcctggcta tcaccgtggg gaaatgccag tgttgacgtc
    51661 aaagtggata acgaaggaca tttcaccggc agtcgtggaa gctggtttgt gccgttacaa
    51721 gataatgatc gttatctcac ctggagccag cttggtctta ctcagcagga tgatgggctg
    51781 gtgagcaatg tgggcgttgg gcaacgctgg gcgcgcggca gctggctggt gggttataac
    51841 actttttatg acaacttgct ggacgaaaat cttcagcgag cgggctttgg cgctgaagcg
    51901 tggggcgaat atttgcgact atcggcaaac ttttatcagc cgtttgctgc atggcatgaa
    51961 cagacagcca cgcaggaaca acggatggcg cgcgggtacg acctgacagc ccggatgcgc
    52021 atgccgttct atcaacacct caataccagt gtcagcgtag aacagtattt tggtgatcgt
    52081 gtcgatttgt ttaactctgg tacgggttat cacaatcccg tcgcgttgag tctgggatta
    52141 aattacaccc ctgtgccctt agtcactgtg acggcccagc ataaacaggg tgaaagtggc
    52201 gagaatcaaa ataacctcgg gctgaatctt aactaccgct ttggtgtacc gctcaaaaaa
    52261 caactttctg cgggcgaggt tgccgaaagt cagtcgttac gtggtagtcg ctatgacaat
    52321 ccgcagcgaa ataatctgcc gactcttgag taccgacagc gaaaaacgtt aacggtgttt
    52381 ctggcgacac ctccgtggga tctaaaacct ggcgaaacag tgccgctgaa attacaaatc
    52441 cgcagtcgtt acggtattcg gcaactgatt tggcagggcg atacgcagat attaagtttg
    52501 acgccgggcg cacaagccaa cagtgaggag ggctggacgc tgatcatgcc tgactggcaa
    52561 aacggggaag gcgcaagcaa tcactggcga ttgtcagtgg tggtggaaga taaccagggg
    52621 cagcgtgtct cctccaatga gatcacgcta acgcttgtcg aaccgttcga cgcattgtca
    52681 aacgacgaac tgcgctggga accgtaatca gaaaatgcgc tcctgatgca cccataccgc
    52741 agcttccacg cgagacttta gcttcatttt cttcagcatg tgcttgacgt gaacttttac
    52801 tgtgctttcg gtgatgtcca ggcggcgggc aatcatcttg ttcggcaaac cctgggcaat
    52861 tagcttgaga atatcgcgct cgcgtggggt taactggtta acatcgcgct cagtagtggc
    52921 acggttagcg cgcaagctgg cggccagaac aggcgttaat gcttcgctta ataccatttc
    52981 gccagcagct gcctgatgta acgctttcag cagatcttcc ggttccatat cttttaacag
    53041 atagccatcc gcgccgcgtt tcagtgcggt gaccacatct tcttcatggt tagagacgct
    53101 gaataccaca atgcgcccgg agagggactt ttcgcgcagt ttatccagcg tttccagacc
    53161 gttcatgccg ggcatattga gatctaacag gatcagatcg ggatcaagag actccgccag
    53221 ttcaataccc tgttcgccat tactcgcttc gccaaccacg gtgatatctg gtgccatact
    53281 gataagctgt tttacgccag ttcgcaacat cgggtgatcg tcgatcagca ggatagtagc
    53341 cggttcctga ttactcatgg gtatctcctt ggacgtctgt gaaagttttt tcgggaataa
    53401 aggtgaccac cacttcggtg ccacctgatt cacgacggcg gacgcggcaa tcgcctcgta
    53461 aactttgcgc acggtcgcgc attattatca tgccgtagtg attgctgcgg atggcatttt
    53521 caggcacgcc gcagccgtta tcctggacgg tcagtttgac ctgattatcg ttttgcgcca
    53581 ccgtcaccac gacctcactc gcttgcgaat gtttgagggc gttacttaat gcctcacggg
    53641 caatttgcaa caagtggatt gcctgatgcg aaggcaccag acgaggcggc aattgataat
    53701 ccagcttcac cgggaagcca aatttggcgc tgtactcttc gcaactcgcc tccagcgccg
    53761 gacgtaatcc aggctcggtg agctgcaaac ggaatgtggt gagcaattca cgcaactgca
    53821 cccaggatgc attcagttcg ttacggatct gacttaacag ttcgcggctg ctttctggca
    53881 gcgcatcgcc ctgcatctgt aagcaactca cctgcatctt catgcaagag agagattggg
    53941 caatagaatc atgcagttcg cgcgcaatgg tggcacgctc ttccatcacg atcaactgtt
    54001 gctgacgttc ctgatggcga tccagcgcca gcgtggcggt gagttgttca accagggtat
    54061 ccaccagttg ttgttgatca tggctaagat gacgcccctg cggcagggtc gccagcaaaa
    54121 taccgtactg cgtatgagtg tcagccagcc gccacttcag ggttgtgccg cgatcgccaa
    54181 cgggtaatac gccgcgcggg cagagctggc agcctttatc atcacaagtc atatctggct
    54241 ggcaggtaaa ctcctgatga ttctcttcat catcagtgtc atacacccgc agttcgatat
    54301 cacgtagcag ggttaaattc tgtaatccgt tgagtacagg tgacaggcgt tcacacagcg
    54361 gggcgcggga atgcaatcgg cggttggcct gccataaaaa agagaggatc tgatttttat
    54421 gctccagccc ggcggttttc tcctgaaccc gctgctcaag tacggcataa ctttcggcca
    54481 gttctgcaga catattgttc aacgcagttc caagcatcgc catttcgttg cgcccgctga
    54541 tgtttgcgcg ttgggtaaaa tcgcgatggc tgacggcact cgccattgcc agcagttgcc
    54601 gccacggttg tagcaatctc gcccgcaacc agataatagt gaataccagt aaaagtgcca
    54661 taaataccgc cattacccga tggaccagca ccactgtctc aatgcgcatt tccgtggtgc
    54721 gatcaaaacc agataccagc tgatcaagcc cggcaacaaa ctggctgaca tccgctgaca
    54781 ccgtttcgcg gttttgtgca cgcatcagtg cagggatcag ttcattacgc cagtaatctt
    54841 gtaaaccctg taattgcgcc agttgcccgt ctcgttctgc tgctcgagtc aactcggcgc
    54901 taaatgctgt ttgttccatc tctttaatta agggcttgtc tttctcgctt aatggcactg
    54961 ctgccaacag acggtaactt tgcatgcgca gcgatcccgc tttgttgatc gcatgggcgc
    55021 tgccctgaac gccttgcacc agccagccag aaaccgccat ccctgccagt ccaatagcag
    55081 tagaaagcaa cacaataagc gcaacctgat taaccagggt gagcggagag agacaacgtt
    55141 taagcatgta aacctcttcc ttcaggcttt aaatgagcaa taaccttaat gaatgtgacg
    55201 atacattctg gaatggcagt attctcggct attggctgaa gtatacccat acccggaaag
    55261 agttactcct tatttgccgt gtggttagtc gctttacatc ggtaagggta gggattttac
    55321 agcaccgtga aaaatctcat aatttttatg aaatcactgt actcactatg gataatgata
    55381 aatatcaatg atagataaag ttatcttatc gtttgattta catcaaattg cctttagcta
    55441 cagacactaa ggtggcagac atcgaaacga gtatcagagg tgtctatgag tcactcatcc
    55501 gcccccgaaa gggctactgg agctgtcatt acagattggc gaccggaaga tcctgcgttc
    55561 tggcaacaac gcggtcaacg tattgccagc cgcaacctgt ggatttccgt tccctgtctg
    55621 ctgctggcgt tttgcgtatg gatgttgttc agcgctgttg cggtgaactt accgaaagtc
    55681 ggttttaatt ttacgaccga tcagctattt atgttgactg cgctgccttc ggtttctggc
    55741 gcgttattac gtgttccata ctcctttatg gttcctatct tcggtggtcg tcgctggacg
    55801 gcgttcagca ccggtattct gattattcct tgcgtctggc tgggttttgc cgtgcaggat
    55861 acctccacgc cttatagcgt cttcatcatc atttctctgc tgtgcggctt tgctggcgcg
    55921 aacttcgcat ccagtatggc aaacatcagc ttcttctttc cgaaacagaa gcagggtggc
    55981 gcgctgggtc tgaatggtgg tctgggcaac atgggcgtca gcgtcatgca gttggttgct
    56041 ccgctggtgg tatcactgtc gattttcgca gtatttggta gccagggcgt caaacagccg
    56101 gatgggactg agctgtatct ggcgaatgcg tcctggatat gggtgccgtt ccttgccatc
    56161 ttcaccattg cggcgtggtt tggcatgaac gatcttgcta cctcgaaagc ctccatcaag
    56221 gagcagttgc cggtactcaa acggggtcat ctgtggatta tgagcctgct gtatctggca
    56281 accttcggct ccttcatcgg cttctccgcg ggctttgcga tgctgtcaaa aacgcagttc
    56341 ccggatgttc agattctgca atacgctttc ttcgggccgt ttattggtgc gctggcgcgt
    56401 tctgcaggtg gtgcattatc tgaccgtctg ggcggaactc gtgtcactct ggtgaacttt
    56461 atcctgatgg cgattttcag cggcctgctg ttcctgacct taccgactga cggacagggc
    56521 ggaagcttca tggcgttctt cgcagtcttc ctggcgctgt tcctgaccgc tgggctgggt
    56581 agtggttcca ctttccagat gatttccgtg atcttccgta aactgacaat ggatcgtgtg
    56641 aaagcagaag ggggttctga cgaacgtgcg atgcgtgaag cggcaaccga cacggcggcg
    56701 gcgctgggtt tcatctctgc gattggcgcg attggtggct tctttatccc gaaagcgttt
    56761 ggtagctcgc tggcattaac gggttcgcca gtcggcgcaa tgaaagtatt tttgattttc
    56821 tatatcgcct gcgtggtgat tacctgggcg gtatatggtc ggcattctaa aaaataaatc
    56881 gtaatatttg attatccttt gcgcggcatg atgtcgcgct ttttttatgc gtcatttagt
    56941 tacaacatac taatgttgta tggcttattt cgctgg
//
LOCUS       DADSZD010000010        54022 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.010, whole genome shotgun sequence.
ACCESSION   DADSZD010000010 DADSZD010000000
VERSION     DADSZD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.010"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            167..376
                     /gene="mokC"
                     /locus_tag="J1Z28_000524"
     CDS             167..376
                     /gene="mokC"
                     /locus_tag="J1Z28_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBA7715388.1"
                     /translation="MQNTCRVPLTDRKVKEKRAMKQHKAMIVALIVICITAVVAALVT
                     RKDLCEVHIRTGQTEVAVFTAYESE"
     gene            complement(481..1611)
                     /gene="dnaJ"
                     /locus_tag="J1Z28_000525"
     CDS             complement(481..1611)
                     /gene="dnaJ"
                     /locus_tag="J1Z28_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBA7715389.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1700..3616)
                     /gene="dnaK"
                     /locus_tag="J1Z28_000526"
     CDS             complement(1700..3616)
                     /gene="dnaK"
                     /locus_tag="J1Z28_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBA7715390.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3989..4393
                     /locus_tag="J1Z28_000527"
     CDS             3989..4393
                     /locus_tag="J1Z28_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBA7715391.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEEQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4419..5132
                     /gene="msyB"
                     /locus_tag="J1Z28_000528"
     CDS             4419..5132
                     /gene="msyB"
                     /locus_tag="J1Z28_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBA7715392.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5281..5847
                     /gene="satP"
                     /locus_tag="J1Z28_000529"
     CDS             5281..5847
                     /gene="satP"
                     /locus_tag="J1Z28_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBA7715393.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6052..6639)
                     /gene="mog"
                     /locus_tag="J1Z28_000530"
     CDS             complement(6052..6639)
                     /gene="mog"
                     /locus_tag="J1Z28_000530"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBA7715394.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6754..7707)
                     /gene="tal"
                     /locus_tag="J1Z28_000531"
     CDS             complement(6754..7707)
                     /gene="tal"
                     /locus_tag="J1Z28_000531"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBA7715395.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7986..9416
                     /locus_tag="J1Z28_000532"
     CDS             7986..9416
                     /locus_tag="J1Z28_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBA7715396.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVAQLMQGFVPLMAIIWVLT
                     CLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSFPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9486..10262
                     /gene="yaaA"
                     /locus_tag="J1Z28_000533"
     CDS             9486..10262
                     /gene="yaaA"
                     /locus_tag="J1Z28_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBA7715397.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10415..10714)
                     /locus_tag="J1Z28_000534"
     CDS             complement(10415..10714)
                     /locus_tag="J1Z28_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBA7715398.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPPRHHKKAHHDHHGGNGPGKHHR"
     gene            complement(10929..12215)
                     /gene="thrC"
                     /locus_tag="J1Z28_000535"
     CDS             complement(10929..12215)
                     /gene="thrC"
                     /locus_tag="J1Z28_000535"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBA7715399.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12216..13148)
                     /gene="thrB"
                     /locus_tag="J1Z28_000536"
     CDS             complement(12216..13148)
                     /gene="thrB"
                     /locus_tag="J1Z28_000536"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBA7715400.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICQLDTAGARVLEN"
     gene            complement(13150..15612)
                     /gene="thrA"
                     /locus_tag="J1Z28_000537"
     CDS             complement(13150..15612)
                     /gene="thrA"
                     /locus_tag="J1Z28_000537"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBA7715401.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     gene            complement(15693..15758)
                     /gene="thrL"
                     /locus_tag="J1Z28_000538"
     CDS             complement(15693..15758)
                     /gene="thrL"
                     /locus_tag="J1Z28_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBA7715402.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15972..16658)
                     /locus_tag="J1Z28_000539"
     CDS             complement(15972..16658)
                     /locus_tag="J1Z28_000539"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBA7715403.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17058..17198)
                     /locus_tag="J1Z28_000540"
     CDS             complement(17058..17198)
                     /locus_tag="J1Z28_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715404.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            17294..18010
                     /gene="arcA"
                     /locus_tag="J1Z28_000541"
     CDS             17294..18010
                     /gene="arcA"
                     /locus_tag="J1Z28_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBA7715405.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18070..19422)
                     /gene="creD"
                     /locus_tag="J1Z28_000542"
     CDS             complement(18070..19422)
                     /gene="creD"
                     /locus_tag="J1Z28_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBA7715406.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRYS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSALVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19480..20904)
                     /gene="creC"
                     /locus_tag="J1Z28_000543"
     CDS             complement(19480..20904)
                     /gene="creC"
                     /locus_tag="J1Z28_000543"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBA7715407.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVASEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20904..21593)
                     /gene="creB"
                     /locus_tag="J1Z28_000544"
     CDS             complement(20904..21593)
                     /gene="creB"
                     /locus_tag="J1Z28_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBA7715408.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21606..22079)
                     /gene="creA"
                     /locus_tag="J1Z28_000545"
     CDS             complement(21606..22079)
                     /gene="creA"
                     /locus_tag="J1Z28_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBA7715409.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22290..23159
                     /gene="robA"
                     /locus_tag="J1Z28_000546"
     CDS             22290..23159
                     /gene="robA"
                     /locus_tag="J1Z28_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBA7715410.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23156..23803)
                     /gene="gpmB"
                     /locus_tag="J1Z28_000547"
     CDS             complement(23156..23803)
                     /gene="gpmB"
                     /locus_tag="J1Z28_000547"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBA7715411.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23855..24382
                     /gene="yjjX"
                     /locus_tag="J1Z28_000548"
     CDS             23855..24382
                     /gene="yjjX"
                     /locus_tag="J1Z28_000548"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP phosphatase"
                     /protein_id="HBA7715412.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEVLGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(24455..24781)
                     /gene="trpR"
                     /locus_tag="J1Z28_000549"
     CDS             complement(24455..24781)
                     /gene="trpR"
                     /locus_tag="J1Z28_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBA7715413.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(24871..26808)
                     /gene="sltY"
                     /locus_tag="J1Z28_000550"
     CDS             complement(24871..26808)
                     /gene="sltY"
                     /locus_tag="J1Z28_000550"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBA7715414.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27015..28682
                     /gene="ettA"
                     /locus_tag="J1Z28_000551"
     CDS             27015..28682
                     /gene="ettA"
                     /locus_tag="J1Z28_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBA7715415.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDSLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28738..29022)
                     /locus_tag="J1Z28_000552"
     CDS             complement(28738..29022)
                     /locus_tag="J1Z28_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004088972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7715416.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(29024..29356)
                     /locus_tag="J1Z28_000553"
     CDS             complement(29024..29356)
                     /locus_tag="J1Z28_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7715417.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(29448..30680)
                     /gene="nadR"
                     /locus_tag="J1Z28_000554"
     CDS             complement(29448..30680)
                     /gene="nadR"
                     /locus_tag="J1Z28_000554"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBA7715418.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNFLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30701..32083)
                     /gene="radA"
                     /locus_tag="J1Z28_000555"
     CDS             complement(30701..32083)
                     /gene="radA"
                     /locus_tag="J1Z28_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBA7715419.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(32132..33100)
                     /gene="serB"
                     /locus_tag="J1Z28_000556"
     CDS             complement(32132..33100)
                     /gene="serB"
                     /locus_tag="J1Z28_000556"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBA7715420.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            33206..33850
                     /locus_tag="J1Z28_000557"
     CDS             33206..33850
                     /locus_tag="J1Z28_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBA7715421.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33878..34894
                     /gene="lplA"
                     /locus_tag="J1Z28_000558"
     CDS             33878..34894
                     /gene="lplA"
                     /locus_tag="J1Z28_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBA7715422.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFTETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWIA
                     GAVR"
     gene            complement(34895..36226)
                     /gene="yjjJ"
                     /locus_tag="J1Z28_000559"
     CDS             complement(34895..36226)
                     /gene="yjjJ"
                     /locus_tag="J1Z28_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HBA7715423.1"
                     /translation="MSELTDLLLQGPRSAPELRQRLAISQATFSRLVAREDRVIRFGK
                     ARATRYALLRPYRGIERIPVWRVDDAGKAHKFADIRLCWPQGSCLVTGADGDERWFDG
                     LPWYLTDLRPQGFLGRAWGRKLAAQLNLTEDIRLWQEEDVLYALTVFSGEYTGGWLVG
                     EGNYQRWITAQRPAAIPLDQKLTHYEQLASDALAGEIVGSSAGGEQPKFTCYAQTPSG
                     NKHVLVKFTVPQQTAVSQRWGDLLIAESIAAQILRDGGIHAIESTVLVTSNRQVFLEA
                     ERFDCKGNDGRLPIVSLEAVQSEFISSPGSWPEAMRRLCEQQLVTHQSVAQTEVIWAF
                     GRLIANSDMHAGNLSFYLSEPPFALTPVYDMLPMAYAPNSAGMLRDAAIEVRFDLNVS
                     KSAWLTAIPLAQQFWQTVAGDPRISEAFRHIAQEMPEKIRQIEGKVARMGG"
     gene            complement(36393..37112)
                     /gene="deoD"
                     /locus_tag="J1Z28_000560"
     CDS             complement(36393..37112)
                     /gene="deoD"
                     /locus_tag="J1Z28_000560"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBA7715424.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(37169..38392)
                     /gene="deoB"
                     /locus_tag="J1Z28_000561"
     CDS             complement(37169..38392)
                     /gene="deoB"
                     /locus_tag="J1Z28_000561"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBA7715425.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPKELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(38444..39766)
                     /gene="deoA"
                     /locus_tag="J1Z28_000562"
     CDS             complement(38444..39766)
                     /gene="deoA"
                     /locus_tag="J1Z28_000562"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBA7715426.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKNDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(39844..40623)
                     /gene="deoC"
                     /locus_tag="J1Z28_000563"
     CDS             complement(39844..40623)
                     /gene="deoC"
                     /locus_tag="J1Z28_000563"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBA7715427.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            40881..42431
                     /locus_tag="J1Z28_000564"
     CDS             40881..42431
                     /locus_tag="J1Z28_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBA7715428.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            42403..43266
                     /gene="yjjW"
                     /locus_tag="J1Z28_000565"
     CDS             42403..43266
                     /gene="yjjW"
                     /locus_tag="J1Z28_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBA7715429.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPCQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(43483..44262)
                     /locus_tag="J1Z28_000566"
     CDS             complement(43483..44262)
                     /locus_tag="J1Z28_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7715430.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(44259..45332)
                     /locus_tag="J1Z28_000567"
     CDS             complement(44259..45332)
                     /locus_tag="J1Z28_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBA7715431.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(45454..45615)
                     /locus_tag="J1Z28_000568"
     CDS             complement(45454..45615)
                     /locus_tag="J1Z28_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBA7715432.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(45742..46347)
                     /gene="osmY"
                     /locus_tag="J1Z28_000569"
     CDS             complement(45742..46347)
                     /gene="osmY"
                     /locus_tag="J1Z28_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBA7715433.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(46741..48330)
                     /gene="prfC"
                     /locus_tag="J1Z28_000570"
     CDS             complement(46741..48330)
                     /gene="prfC"
                     /locus_tag="J1Z28_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBA7715434.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(48421..49098)
                     /gene="yjjG"
                     /locus_tag="J1Z28_000571"
     CDS             complement(48421..49098)
                     /gene="yjjG"
                     /locus_tag="J1Z28_000571"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBA7715435.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(49113..49559)
                     /gene="rimI"
                     /locus_tag="J1Z28_000572"
     CDS             complement(49113..49559)
                     /gene="rimI"
                     /locus_tag="J1Z28_000572"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBA7715436.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LKQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(49528..49941)
                     /gene="holD"
                     /locus_tag="J1Z28_000573"
     CDS             complement(49528..49941)
                     /gene="holD"
                     /locus_tag="J1Z28_000573"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBA7715437.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VTSPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            50044..51075
                     /gene="rsmC"
                     /locus_tag="J1Z28_000574"
     CDS             50044..51075
                     /gene="rsmC"
                     /locus_tag="J1Z28_000574"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBA7715438.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRCGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANSVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            51266..51352
                     /locus_tag="J1Z28_000575"
     tRNA            51266..51352
                     /locus_tag="J1Z28_000575"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51300..51302,aa:Leu,seq:cag)
     gene            complement(51391..51627)
                     /locus_tag="J1Z28_000576"
     CDS             complement(51391..51627)
                     /locus_tag="J1Z28_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBA7715439.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            51768..52556
                     /gene="fhuF"
                     /locus_tag="J1Z28_000577"
     CDS             51768..52556
                     /gene="fhuF"
                     /locus_tag="J1Z28_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBA7715440.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(52594..53271)
                     /gene="bglJ"
                     /locus_tag="J1Z28_000578"
     CDS             complement(52594..53271)
                     /gene="bglJ"
                     /locus_tag="J1Z28_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBA7715441.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRYCET
                     SNVLHPY"
     gene            complement(53229..53954)
                     /locus_tag="J1Z28_000579"
     CDS             complement(53229..53954)
                     /locus_tag="J1Z28_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA7715442.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHSERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        12770 a        14642 c        13457 g        13153 t
ORIGIN      
        1 caaaaatgcg aggcgtcttc agaaaaagaa ttgataaaac agaagtgaat taaaagcgca
       61 gtataatagc ggcgggtgct tgaggctgtc tgtctcaggc attggctgaa cggcagatag
      121 agaaaagccc cgagtgatat tttaccatca acccgaggcc ccctatatgc agaacacatg
      181 tagagtgcct cttactgacc gaaaggtcaa ggagaagaga gcaatgaagc agcataaggc
      241 gatgattgtc gccctgatcg tcatctgtat caccgccgta gtggcggcgc tggtaacgag
      301 aaaagacctc tgtgaggttc acatccgaac tggccagacg gaggttgctg ttttcacggc
      361 ttacgaatcc gagtaagagc aacggcgggg agtgatcccc gccactttac aggtgctcgc
      421 atatcttcaa cgcaccctgg tttttaccct ggcctgccca cgggcaggct tttggggagg
      481 ttagcgagtc aggtcgtcaa aaaacttctt cacgccatca aagaagctct ttgaacgcgg
      541 gctgttgtgc tcgccggttg ggccaccaaa gctttcttgc agctcttgca gcagctgttt
      601 ctgcttctcg ttcaaaccta ccggtgtttc gacaacaacg cggcatagca aatcaccctg
      661 tgcgccaccg cggacagact tgacgccttt accgcgcata cggaacagct taccggtctg
      721 ggtttcgcca ggcactttca gtttgacgcg accatcaagg gtcggtactt cgatttcacc
      781 acccagcgcc gccatagcga agttgatcgg gacttcgcaa tacaggttgt tgccttcacg
      841 ctcgaaaatc gggtgctgtt taacctgaac ctgaacgtac agatcgcctg ccggtgcgcc
      901 gtgttcaccc gcttcacctt cgcccgcaag acggatgcgg tctccagtgt ccacccctgc
      961 cgggatttta acggacagcg ttttgctgcg ctcaacacga ccatgaccat gacatttgtt
     1021 gcacggatct ttgatcagcg taccgcggcc ctgacagtgt ggacaggtct gctgcacggc
     1081 aaagaaaccc tggcgcatct gcacctggcc agaaccatga caggtcggac aggtctgcgg
     1141 ctgtgtacct ggttttgcac cgctaccgtg gcaaacgtca cactcttcca gagtcggaat
     1201 gcggatctct ttggtcacgc cacgtacagc ttcttcgagg gtgagctcca tgttatagcg
     1261 taaatcagca ccgcgcgccg cacgttgacg accacgtccg ccgccaaaaa tatcgccgaa
     1321 aacgtcacca aaaatatcgc tgaagtctgc gccgccgcca aaaccgccgc cgcccatgcc
     1381 gccttgctca aacgcagcat gaccatactg atcgtatgcc gcacgttttt gcgagtcggt
     1441 cagaacttca taagcttcct tgatctcttt aaatttcgcc tcggcctctt tgtcaccctg
     1501 gttacggtcc gggtggtatt tcatggccag gcgtttgtag gcctttttga tttcacgctc
     1561 ttccgctgtt ttggaaacgc ctaaaatctc gtaataatct tgcttagcca tcttttttga
     1621 tttgccccta gatgaatgca cgggcggaga ggaaattccc cttcgcccgt gtcagtataa
     1681 ttacccgttt atagggcgat tattttttgt ctttgacttc ttcaaattca gcgtcgacaa
     1741 catcgtcatc tttcgcgttg tttgcagaag catcagcacc ggcagtctgc tgctgggcat
     1801 gttgctgctg ggcgatttcc atcagtttct gggaaacctg tgccagttcc tgcattttcg
     1861 cttcgatagc ggctttgtct tcacctttca gagcagtttc cagtgcagtc agcgcagact
     1921 cgatagcagt tttgtcgtca gccggcagtt tgtcgcctgc ttcttcaacc tgcttacggg
     1981 tgctgtgcag cagatggtcg ccctggttgc gagtctgtac cagctcttca aacttacggt
     2041 cagcttcggc gttagcttct gcgtcgcgta ccattttctg gatttcatct tcgttcagac
     2101 cagaagaagc cttgatagtg atcttctgct ctttaccgct gtttttgtct ttcgcggaaa
     2161 cgtgcaggat accgtcagca tcgatatcga aggtaacttc gatctgcggc atgccgcgcg
     2221 gtgccgggtt gataccatcc aggttgaact gacccagaga tttgttatca gccgcacgtt
     2281 tacgttcacc ctgcagcaca tggatggtta ccgcagactg gttgtcttca gcggtagaga
     2341 acacctggct gtgcttggtc gggatagtgg tgtttttcgc gatcagcgtg gtcatcacac
     2401 cgcccatggt ttcgataccc agagacagcg gggtaacgtc cagcagcagt acgtctttta
     2461 cgtcaccagt cagaacacca ccctgaacag cagcaccgat ggctacagct tcgtccgggt
     2521 taacatcttt acgcggctct ttaccaaaga attcagcaac tttcttctga accattggca
     2581 tacgagtctg accaccaacg aggataacgt cgtcgatatc agatacggac aggccagcgt
     2641 cctgcagtgc aactttcagc ggctcaatgg aacggtttac cagatcttca accaggcttt
     2701 ccagtttcgc acgagtcact ttgatgttca tgtgtttcgg accggtcgcg tctgcagtga
     2761 tgtacggcag gttaacgtcg gtctgctgag cggaagacag ttcgattttc gctttttctg
     2821 ccgcttcttt caggcgctgc attgccagcg gatcgttgcg caggtcaatg ccctgatctt
     2881 tcttgaattc ttcaaccaga tagttgatca gacgactgtc gaagtcttca ccacccaggt
     2941 gggtatcacc gttggttgcc agaacttcga aggttttttc gccgtcaact tcgtcgattt
     3001 cgataatgga aatatcgaaa gtaccaccac ccaggtcata aaccgcgata gtacggttgc
     3061 cagtaccttt gtccagaccg taagccagcg cagctgcggt cggttcgttg atgatacgtt
     3121 ttacttccag accagcgata cggcctgcgt ctttggttgc ctgacgctga gcatcgttaa
     3181 agtatgccgg tacggtaata acagcttcag ttaccggttc acccaggtaa tcttcagcgg
     3241 ttttcttcat ttttttcagc acttcagcag agatctgcgg cggtgccatt ttctggcctt
     3301 taacttcgac ccatgcgtcg ccgttatcag cagcaataat tttgaacggc atgatggaaa
     3361 catcacgctg tacttcttcg tcctggaagc ggcggccaat caggcgttta atcgcaaaca
     3421 gggtgttttg cgggttcgtc actgcctgac gtttagccgg ctgaccaacc agagtttcac
     3481 catcctgggt ataggcaatg atagaaggcg tggtgcgatc gccttcggcg ttctccagta
     3541 cacgaggagt ggtgccatcc ataatcgcta cacaagagtt ggtagtaccc aggtcgatac
     3601 caattatttt acccatctaa acgtctccac tatatattcg gtcatcatgt ggttgtgagt
     3661 ctgtaatagg ggcgaaacgt ctggtttcaa ctgcccaatt tcattttttt tgcagactca
     3721 ctcactgcgg ttgactacta aatggggtcg taacccacgt catcaagggg gagatgcaaa
     3781 aattttttgt gcttttgcgc agaaatttcg cgatatggca ggacaagacg tgaaactgac
     3841 aacaatttta ttcatataaa gaatgaatcg cagctaaggc attcatcaaa aaattgtaat
     3901 aaaaagaaaa tattacgtac ctgaaacttc tctttatcag cagtaaacta gtttcatccc
     3961 cttacccctc cccaccaaga gaatccttat gaaatccgtt attacgattt ccgccagcct
     4021 ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaaa tcctcggcgt
     4081 cattgcaatg ccgcgaaacg aaaccaacga tctggcgctg aaacttcctg tttgtcgcat
     4141 tgttaaacgc atacaactct cagccgacca tggcgatttg cagttaagcg gtgcatcgat
     4201 ttatttcaaa gccacccgaa gcgccagtca gaccctgaat attccttcag aaataaaaga
     4261 agagcaaacc actgactgga tcaacattaa cagcgataac gacaacaaac gctgcgtctc
     4321 aaaaatcacc ttttccggtc atacagtgaa ctcatcggat atggccacgc tgaaaattat
     4381 cggcgacgac taacgccaga atatagaaac cacaaaaaat gaatgttaat tacctgaatg
     4441 attcagatct ggattttctc cagcattgta gtgaggaaca gttggcaaat ttcgcccggc
     4501 tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg cgcaacgaac
     4561 tgtttaaatc gatggaagga catcccgagc gacaccgccg caactggcag ctgattgccg
     4621 gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga cacggtaaat
     4681 tgtatcgcgc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc gacaaagaga
     4741 tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat acctggaaga
     4801 aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg gtgaatgagc
     4861 tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt gtgtcgcatt
     4921 tgctttccag ccaattgacc cgcattttac gcacccacgc agcaatgagc gtgcttgggc
     4981 atggtttgct gcgcggcgcg ggtctgggag gccctgtggg cgcagcatta aatggggtta
     5041 aagcggtcag cggcagtgcc tatcgcgtga caattccagc cgtactgcaa atcgcctgcc
     5101 tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa attttcattt
     5161 tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc gccctgaaaa
     5221 cactacagtt attcagggaa attatttcac cattcattcg atgatgattt ttgaggaatt
     5281 atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt cggcatgacc
     5341 accattctgc ttaacctgca caacgtgggt tattttgctc tggacggtat tattcttgcc
     5401 atgggcattt tctacggcgg catcgcgcaa atttttgccg gtctgctgga gtacaaaaaa
     5461 ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct gacgctggtt
     5521 gcaattctgc tgatgccgaa actggggctg accgatgcgc caaatgccca gttccttggt
     5581 gtctacctgg gtctgtgggg catatttacg ctgtttatgt tcttcggcac gctgaaaggc
     5641 gcacgcgttc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct gctggcgatc
     5701 ggtaacattg ccggtaacgc cgcgatcatc cactttgccg gctggattgg tcttatctgc
     5761 ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt tggtcgcacc
     5821 gttctgccga ttggtgagtc tcactaattg ttaagctccc cccgctcgaa gggataagat
     5881 tgttaacaac gtgcgctttg tttatgccgg atgcggcgtg aacgccttat ccggcctaca
     5941 aaatcgtgca aattcaatat attgcaagaa gatcgtaggc ctgataagcg tagcgcatca
     6001 ggcagttttg cgtttgtcat cagtctgatc cccccgcctg gggggatttt tttattcgct
     6061 aacttcgcgt cttgcactct tcggtctgaa tgctgcaacc acttccggtg ccgtttcaac
     6121 gtatggccct tccagcaact gaatgcagta cggtacgctg gcaaaaatac cgtgcaccac
     6181 aacgttaccc tcagcgtcct tcacaccttc cagcgtctct ttaatagact tcggttgacc
     6241 gggtaagtta aggatcagcg cctgtttgcg aatcaccccc acctgacgcg aaaggatcgc
     6301 agttggtaca aaatgcaggc tgatctggcg catctgttca ccaaagcctg gcatctcgcg
     6361 gtccgctact gccagcgtcg catcgggcgt tacgtcacga cgcgcagggc cagttccgcc
     6421 cgtggtgagc accagatggc aactcatttc atccaccagc tcacacaacg tttgctcgat
     6481 gatcgcctgc tcatcgggga ttaagcgggt ttccagttca aacggcgtgg ttagcgccga
     6541 tgtcagccat tcttccagcg cagggatgcc tttatcctga taaacgccgc tggatgcgcg
     6601 atcagagatg gaaactaagc caatacgtaa agtattcatc aagatttttc cgtttaaaca
     6661 gtctcgttaa acagaatgat acactgcgaa gggagtgaca gacaggtttc agaagaggta
     6721 gcgtgaccga cttcccggtc acgctaagta tgattacagc agatcgccga tcattttttc
     6781 cagtttttcc tggtcaacag caaacttacg gataccttcc gccagtttat ctactgccat
     6841 tggatcctgg ttgtgctgcc acaggaactc ggactcagtg atacgcgccg gacgcgcttt
     6901 cacttcacca gtgtaagaca gtttacgttc gatagcccct tcgctctccg ccagctcttt
     6961 cagcagtgcc ggtgcgatgg tcagacggtc gcagcctgcc agttccagaa tttcgccgat
     7021 gttacggaag cttgcgccca taaccacggt ttcataacca tgctctttgt agtactggta
     7081 gatttcagat acagaaacca cgcccggatc ttctgccgga gcgtactctt tcttatcggt
     7141 attcgctttg taccagtcaa gaatacggcc aacaaacggc gagatcagga acacgcccgc
     7201 ttccgcacaa gcacgagcct gagcgaagga gaacagcagg gtcaggttac agttaatacc
     7261 ttctttttcc agctgttctg cagcacggat accctgccag gtagaagcca gtttgatcag
     7321 aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg atcaggcgtt ttgctttcgc
     7381 aattgacgct tcggtgtcat aggaaagacg cgcatcaact tcagttgaga tacggcccgg
     7441 aaccagtttc aggatttcca gaccaatatt tacggccagt ttgtcggtcg cgtccacgat
     7501 ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg acagcatcat caatcaactt
     7561 acggtattcc ggaatctgcg ctgcgttaag aatgagagaa gggttggttg tggcatcctg
     7621 cggttgatac agcttcattg ccgctatgtc cccagtgtcg gccactacgg tggtgtactg
     7681 acgaagggag gtcaatttgt ccgtcatgat agtatttctc tttaaacagc ttgttagggg
     7741 gatgtaaccg gtctgccctg atgatatcac gacgcaacgt cagcgcaacc gccgacaaga
     7801 cggttatcgc caggcgagac tgtttcggat ttctgacact gcgttgatat cgcccgccat
     7861 ttttatacaa aacctcatgt atgctacgca gaagttatca agtacctcgt agcgtatata
     7921 cttcttaaac aattggtaac gtttacacag gaaagtcatc gcgaccggca ataagaggga
     7981 tatgcatgcc agattttttc tcctttatta acagcgtcct ttggggatcg gtaatgattt
     8041 acctgctctt cggcgcaggt tgttggttca cttttcgcac cggatttgtg cagtttcgct
     8101 acatccgcca gtttggcaaa agtcttaaaa atagcattca tccacagcca ggcggtttaa
     8161 cctcatttca gtcattgtgt accagtcttg cagcgcgcgt gggtagcggc aacctggccg
     8221 gcgttgcgct ggcaattacc gccggtgggc ctggagccgt cttctggatg tgggttgccg
     8281 catttatcgg catggcgacc tcgtttgccg aatgttccct cgcacagctt tataaagaac
     8341 gtgatgccaa tgggcagttt cgtggcggac cggcatggta tatggcgcgc gggctgggga
     8401 tgcgctggat gggcgttctg ttcgccgtct ttttgctcat cgcctacggc ataattttca
     8461 gcggagttca ggcgaacgcc gttgcgcgag ccctgagttt ttcttttgat tttcccccgc
     8521 tggtgacagg tattattctc gctgtctttg ctctgctggc gatcactcgc ggtcttcatg
     8581 gcgtcgccca gctcatgcag gggtttgtcc cgttgatggc gataatctgg gtactgacct
     8641 gcctggtgat ttgcgtaatg aatatcgggc aacttcccca cgtcatttgg tctatttttg
     8701 agagtgcttt tggctggcag gaagctgcag gcggcgcggc gggatatacc ttaagccagg
     8761 cgattactaa cggttttcag cgcagtatgt tttccaatga ggcgggaatg gggtcgacgc
     8821 caaacgcggc agcggcagct gcgtcctggc ctccgcatcc ggcagcgcaa ggaattgtcc
     8881 agatgattgg catttttatc gacaccctgg tcatctgcac ggcaagcgcc atgctgatat
     8941 tactggcggg taacggcaca acctacatgc cgctggaagg tattcagctt atccagaagg
     9001 cgatgcgggt gctaatgggt tcctggggtg cggagtttgt taccctcgtg gttattctgt
     9061 ttgccttcag ctccatcgtt gccaactaca tttatgccga aaacaatctc ttctttttac
     9121 gcctgaacaa ccctaaagcg atctggtgtc tgcggatctg caccttcgca acggtcatcg
     9181 gcggcacctt gctaagtttt ccgctgatgt ggcaactggc agatatcatt atggcctgca
     9241 tggctattac caatttgacc gccatattac tgctctcgcc tgtggttcat accattgcca
     9301 gtgattatct acgccagcgt aaactcggcg tgcgcccggt gtttgatccg ttgcgttatc
     9361 cggagatcgg tcgccagctt tctccagacg cgtgggatga tgtttcgcag gagtaatcac
     9421 aactatcgat caactcattc tctttttttg ctaaagtcgg cataaatttc ctgcaaggac
     9481 tggatatgct gattcttatt tcacctgcga aaacgcttga ttaccaaagc ccgttgacca
     9541 ccacgcgcta tacgctgccg gagctgttag acaactccca gcagctgatc catgaagcgc
     9601 ggaaactgac gcccccgcag attagctccc tgatgcgcat cagcgacaaa ctggcgggta
     9661 tcaacgccgc tcgctttcat gactggcagc cagatttcac accggagaat gcccgccagg
     9721 cgattctggc gtttaaaggt gatgtctaca ccggcttgca ggccgaaacc ttcagcgaag
     9781 acgatttcga ttttgcccaa cagcatttgc gaatgctttc cggcttgtat ggcgtactcc
     9841 gcccgctcga tttaatgcag ccttatcgtc tggaaatggg gatccgtctt gagaatgccc
     9901 gagggaaaga tctgtatcaa ttctggggag atatcatcac caacaagctg aacgaggcgc
     9961 tcgcagcaca aggcgataat gtggtgatta atctggcgtc agatgagtat tttaaatcgg
    10021 tgaagccgaa gaaattgaat gccgagatta tcaagccggt gttcctcgat gagaagaacg
    10081 gcaaatttaa gatcatcagc ttctacgcta agaaagcacg cggtctgatg agtcgtttca
    10141 ttattgaaaa tcggctgacc aaaccagagc aactgacagg ttttaatagc gaaggttact
    10201 tctttgatga agcttcctcc agcaatggcg aactggtgtt taaacgctac gagcagcgtt
    10261 aagtttgtag tcaatgtcgg atgcggcgtg aacaccttat ccggcctaca aaagcatgca
    10321 gattcaatat attgcagaat tcacgtaggc ctgataagac gcgccagcgt cgcatcaggc
    10381 gctgaatgcc ggatagttac ccggcatttg ttatttagcg gtgatgtttg cctggaccat
    10441 taccgccgtg atgatcatga tgagctttct tatggtgacg cggcggcggt ggcggcggtc
    10501 cgtgtgggtg ccagcgattg cctcgccact cataatgttg tttccaccag ccgtggtcgc
    10561 gccagtgacc gccatcccag taatagccac gattatcacg atcgcctatc tgtaatttta
    10621 ctgacgggac taacgtaatt tccgcagcct gcgctgccat gggagcgacc agaaccaggg
    10681 aaagtgcgag tacgatagat tgcatctttt tcacaggtta ctccttttat cattgcccgt
    10741 tatgggtcga tatccggaga ttacgcgatg agaacggcag ttgttattct ccgcaatcct
    10801 aatctctaag ttaccgcatt tattgagaat ttctcctttt tccctgtcgt ttttctccat
    10861 attttcttca taaaaaaagc cgggctgcat aaaagcaaac ccggcctgat tgagataatg
    10921 aatagatgtt actgatgatt catcatcaat ttacgcaacg cagcaaaatc ggcgggcagg
    10981 ttatgcgaaa gcaagggtaa atcagcacgt tctgccagct cttttggcag atccaacgtt
    11041 tcaccgagaa tcgcttccac gctctcttta aatttcgccg gatgcgcggt gccgaggaac
    11101 aagccatatt cgcctggatt caactggtca cgcagcgcac gataagctac ggcagcgtgc
    11161 ggctccgagg tatagcccag ttcttttaac tcacgcattg tctgttgcgt ggtttcatca
    11221 tccacggctg cataccccag ctctttcagt tgccagattt tgcggcggaa caactcttcc
    11281 acacgcggcc agttgtttgg ctggctaaca tccatcgcat tggataacgt cgcctgagtc
    11341 gctttgggtg accactgacc gtcgtgcagg aaacgtggta cggtatcgtt cacgttggtc
    11401 gcagcaataa aacgcttcac cggcagaccg agtgacttcg ccagcaggcc tgccgttaaa
    11461 tcaccgaagt ttccactcgg taccgagaca accaactggt tacgtgcttc ttgcggcaac
    11521 tgtgcgacag cctcaaagta ataacaaatc tgcgccagca atcgactgat gttgatggag
    11581 ttagcagaat tcagccccag cgccactttc agttcttcat catcaaacgc ctgcttcacc
    11641 agcgcctgac aggcatcgaa atcgccgtcg atggcaacag tttcgatatt gccgcccaat
    11701 gtacagaaca gtttttcttg cagtggactg attttgcctc gtggatagag gataaccact
    11761 ttcacattcg gtaaaccgta gaaagcatga gccactgccg ctccagtatc accggatgtc
    11821 gcggtcagaa tggtcactgg cttatcgccc gcaatatggg tcagcatttg tgccataaag
    11881 cgaccgccga aatctttaaa tgccagcgtt ggcccgtgga acaattccag acaaccgaca
    11941 tcgctttcaa cattggcgac cggagccggg aaggcaaacg ccgcacgtac gcgctcttcc
    12001 aggatttcct gcgggatttc atcaccaata aacgccgaga ggatcttcgc actgcgggtg
    12061 acaaaatcca gcttcagcat ctcatcaatt tcagtcaggc tgaattccgg caggtcgtgc
    12121 ggaaaaaaca gcccctgatt tttgcctaac ccctgggtta cggcttgcgc aaagctgacc
    12181 tgctcattgt gatctttcag attgtagagt ttcatttagt tttccagtac tcgtgcgccc
    12241 gccgtatcca gctggcaaat atgaacaaaa ccttcctgat tttgcaggta gttcttaccc
    12301 aaccagtcgg caacgcgctg ggcggtatcc ggcttgtcac acagagcgaa caaggtcggg
    12361 ccggagccgg agataccgct cgctaccgcg ccgatttccg caaccgcctg ccgcgcctgc
    12421 cggaagccag gcaataaccg ttcacggtag ggttcagcga taacatcttt catcagcttc
    12481 gcggcaagct caggctgacg ggaatagcag gcgtgaatga agcctgccaa atgtcgcccg
    12541 tgcgcaatgc aatcctggcg gcgatactgc gccggtaaaa tagccctagc ttctgccgtc
    12601 gagactttaa tccccggata cgccagcacc cacagccact catcaaaccc aggcacttgc
    12661 tggctgatga tgtcgttttc ttcgatcatc aactgcatac caccaagaaa acacggtgcc
    12721 acgttgtcgt aatgaatgct gccggagata cgcccttcca gctcgcccat caaagccagc
    12781 aaacgagtgt cattaagcgg cttgccacag tgttcattca tcgccatcag cgccgcgacc
    12841 accgaacagg cgctggagcc taagcccgaa ccgatcggca tattcttttc cagagtcatc
    12901 gccactggaa tttgcttgcc aagctcctgg caaaaacgct cccagcactg ataaacgata
    12961 ttttcccgtg gctctgacgg cagcttatcg gcaaagcgtc cgaggttgtt gagactgaat
    13021 gtctctgccg cctcaaccgt gactacatct ccgagcaatg caccatcaac aggtgtcacc
    13081 gccgccccga gcacatcaaa cccgacgctc atattggcac tggaagccgg ggcataaact
    13141 ttaaccatgt cagactccta acttccatga gagggtacgt agcagatcgg caaagacacc
    13201 ggcagctgta acgtcattgc ccgcaccata tccgcgcagt accaacggca gcggctgata
    13261 atagtggcta tagaaggcca gggcgttttc gccatttttc actttgaaca gcggatcatt
    13321 accatccact tcggcaatct tcacgcggca gacgccatct tcatcaatat tgccaacata
    13381 gcgcaaaact tttccttcat cacgggcctt cgccacgcgc gcggcaaaga gatcgtcgag
    13441 ctgtgacaga ttcgccataa aagcggcaac atcaccctcg gcgttaaact ctgcgggcag
    13501 cacaggttca atttcaatat ccgccagctc cagttcacgt cccgtttcac gagcgagaat
    13561 caatagttta cgcgccacat ccataccaga aagatcatct cgcgggtccg gttcggtata
    13621 acccatttcc cgcgccagcg tggtcgcctc ggagaaactc atgccttcgt ctaacttgcc
    13681 gaagatataa gaaagcgaac cagaaagaat gccggagaac ttcatcaatt catcacctgc
    13741 attgagcaga ttttgcaggt tctcaataac cggtaatcca gccccaacgt tggtgtcata
    13801 gaggaattta cgccgcgatt tttccgccgc atgacgcaac agatggtagt aatccatcga
    13861 cgaggtgtta gcctttttgt tcggcgtaac aacgtggaaa ccttcgcgca ggaagtcggc
    13921 atattgatcc gccactgcct ggctggaagt acagtcaaca atgaccgggt tcagcagatg
    13981 atattctttc acgaggcgaa ttaagcgccc gagattaaac ggctctttgg cttgcgccag
    14041 ttcttcctgc cagttttcca gattaaggcc atgtacattg gtgagcagtg ccttcgagtt
    14101 agcaacaccg cagacacgta agtcgatatg tttattcttc aaccagcttt gctgacgctt
    14161 cagttgctcc agcagcgcac cgccaacgcc accgacgcca atcacaaaca cttcgataac
    14221 ctgatcggta ttgaacagca tctgatgagt aacgcgcacg ccagtggtcg catcatcgtt
    14281 attgaccacg acagagattg agcgttcaga agatccctga gcaatggcga caatgttgat
    14341 attggcgcgg gccagcgcgg caaagaattt cgccgagatc ccacgtaagg tgcgcatacc
    14401 atcacctacc accgagataa tggccagccg ttccgtcacc gccaacggct ccagtaagcc
    14461 ttctttcagt tccaggtaga actcttcctg cattgcccgt tcagctcgca cacagtcgct
    14521 ttgcggaacg cagaaactga tactgtattc ggaagatgat tgcgtaatca gcaccacgga
    14581 aatacgggcg cgtgacatcg ctgcaaagac gcgcgccgcc atgccaacca tccctttcat
    14641 ccccgggccg gaaacgctga acattgccat gttattcaga ttggaaatgc ccttgaccgg
    14701 taattcgtct tcatcacggc tggcaccaat gagcgtacct ggtgcttgag gatttccggt
    14761 atttttaatc aggcaaggga tctggaactg ggcgatgggg gtaatggtgc gggggtgaag
    14821 aactttagcg ccgaagtaag aaagctccat cgcttcctga taggacatcg acttcaacaa
    14881 cctcgcatcg ggcacctgac gcggatcgca ggtataaaca ccgtcaacat ccgtccagat
    14941 ctcgcaacaa tcggcgcgta aacaggccgc cagcaccgca gcggagtagt cggaaccgtt
    15001 gcgtcccaga accaccagct cgcctttttc attaccggca gtgaaaccag ccatcagcac
    15061 catgtggtca gccggaatgc ggcttgccgc aatacggcgg gtggattcag caatatcaac
    15121 ggtagattcg aggtaatgcc ccactgcaag cagtttttcg accggatcga taacggtaac
    15181 gttgtgacca cgcgcttcta acacgccggc cataatggcg atcgacattt tctcgccacg
    15241 gcaaatcagc gcagcgttga tgctatccgg gcactgcccc aacaaactga tgccatgcag
    15301 gacatgtttt atttgggcaa attcctggtc gacgaaagtt ttcaattgtg ccagcggaaa
    15361 tcccggctgg gcggcggcga gtcccgtcag aagttcggca aaaatacgtt cggcatcgct
    15421 gatattgggt aaagcatcct ggccgctaat ggttttttca atcatcgcta ccagatggtt
    15481 ggtgattttg gcgggggcag agaggacggt ggccacctgc ccctgcctgg cattgctttc
    15541 cagaatatcg gcaacccgca gaaaacgttc tgcatttgcc actgatgtac cgccgaactt
    15601 caacactcgc atggttgtta cctcgttacc tttggtcgaa aaaaaagccc gcactgtcag
    15661 gtgcgggctt ttttctgtgt ttcctgtacg cgtcagcccg caccgttacc tgtggtaatg
    15721 gtgatggtgg tggtaatggt ggtgctaatg cgtttcatgg atgttgtgta ctctgtaatt
    15781 tttatctgtc tgtgcgctat gcctatattg gttaaagtat ttagtgacct aagtcaataa
    15841 aattttaatt tactcacggc aggtaaccag ttcagaagct gctatcagac actctttttt
    15901 taatccacac agagacatat tgcccgttgc agtcagaatg aaaagctgaa aatcacttac
    15961 taaggcgttt tttatttggt gatatttttt tcaatatcat gcagcaaacg gtgcaacatt
    16021 gccgtgtctc gttgctctaa aagccccagg cgttgttgta accagtcgac cagttttatg
    16081 tcatctgcca ctgccagagt cgtcagcaat gtcatggctc gttcgcgtaa agcttgcagt
    16141 tgatgttggt ctgccgttgt gtcacttttc gccggttgtt gtattaatgt tgctaattga
    16201 tagcaataga ccatcactgc ctgccccaga ttgagcgaag gataatccgc caccatcggc
    16261 acaccagtaa gaacgtcagc caacgctaac tcttcgttag tcagcccgga atcttcgcga
    16321 ccaaacacca gagcggcatg gctcatccat gaagattttt cctctaacag cggcaccagt
    16381 tctactggcg tagcgtagta atgatatttc gcccgactgc gcgcagtggt ggcaacagtg
    16441 aaatcgacat cgtgtaacga ttcagctagt gtcttgaaaa ctttaatatt atcaataata
    16501 tcaccagatc catgtgcgac ccaacgggcg actggctcca ggtgtgcctg actatcaaca
    16561 atccgcagtt cgctaaaacc catcgttttc attgcccgcg ccgctgcccc aatattttct
    16621 gctctggcgg gtgcaaccag aataatcgtt atacgcatat tgccactctt cttgaccaaa
    16681 taaccgcgaa cagggtgatc attgtcaact tattacgcgt tgcgaattta caaattctta
    16741 acgtaagtcg caggaaaagc cctttgctta gcttaaaaaa agctaaacta tttcctgact
    16801 gtactaacga ttgaattgtt aaaaaatgct acatatcctt ctgtttactt aggataattt
    16861 tataaaaaat aaatctcgat aattggattc accacgttta ttagttgtat gaagcaacta
    16921 gttggattat taaaataatg tgacgaaagc tagcatttag atacgatcat ttcatcaaac
    16981 tgttaacgtg ctacaattga acttgatata tgtcaacgaa gcgtagtttt attgggtgtc
    17041 ctgcccctct tagcctgtta tattgctgtt aaaatggtta ggatgacagc cgtttttgac
    17101 actgtcgggt cctgagggaa agtacccacg accaagctaa tgatgttgtt gacgttgatg
    17161 gaaagtgcat caagaacgca attacgtact ttagtcatgt tacgccgatc atgttaattt
    17221 gcagcatgca tcaggcaggt cagggacttt tgtacttcct gtttcgattt agttggcaat
    17281 ttaggtagca aacatgcaga ccccgcacat tcttatcgtt gaagacgagt tggtaacacg
    17341 caacacgttg aaaagtattt tcgaagcgga aggctatgat gttttcgaag cgacagatgg
    17401 cgcggaaatg catcagatcc tctctgaata tgacatcaac ctggtgatca tggatatcaa
    17461 tctgccgggt aagaacggtc ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc
    17521 gttgatgttc ctgactggcc gtgacaacga agtcgataaa attctcggcc tcgaaatcgg
    17581 tgcagatgac tacatcacca aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa
    17641 cctgctgtcc cgtaccatga atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag
    17701 ctacaagttc aatggttggg aactggacat caacagccgt tcgttgatcg gccctgatgg
    17761 cgagcagtac aagctgccgc gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc
    17821 aggcaaaatt cagtcccgtg ctgaactgct gaagaaaatg actggccgtg agctgaaacc
    17881 gcacgaccgt actgtagacg tgacgatccg ccgtattcgt aaacatttcg aatctacgcc
    17941 ggatacgccg gaaatcatcg ccaccattca cggtgaaggt tatcgcttct gcggtgatct
    18001 ggaagattaa tcggctttac caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat
    18061 ttttctacct tatttccaga tacgtaattc atcgtccgtt gtaacttctt tactggcttt
    18121 cattttcggc agtgaaaacg cataccagtc gatattacgg gtcacaaaca tcatgccggc
    18181 cagcgccacc accagtgcac tggttcccaa caacagcgcg ctatcggcag agttgagcag
    18241 tccccacatc acaccatcca gcaacaacag cgcgagggta aacaacatgc tattacgcca
    18301 gcctttcaat accgcctgca aataaatacc gttcattaac gccccaatca gactggcgat
    18361 tatccatgcc acggtaaaac cggtatgttc agaaagcgcc agcaagagca aataaaacat
    18421 caccaatgaa agccccacca gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt
    18481 ttcaaaaaca aagaatgcca taaaagtcag cgcaatcagc agaatggcgt acttagtcgc
    18541 ccggtccgtt aattggtatt gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa
    18601 gttttcccag ccggtatcat tgcctgaagc aaaacgctca ccgagattat tagcaaacca
    18661 gctactttgc cattgcgcct gaaaacctga ctcgctaact tcccgtttgg ctggtagaaa
    18721 atcacctaaa aaactgggat gcggccagtt gctggttaag gtcatttcgc tataacgccc
    18781 gccaggcacc acagaaagat cgccggtacc gcttaaattc agcgccacat tcagcttcag
    18841 gttctgcttg cgccagtccc cttcgggtaa agggatatgc acgccctgcc cgccttgctc
    18901 taacccggtg ccgggttcaa tggtcagcgc cgtaccgtta acttcaggcg ctttcaccac
    18961 accaatacca cgcgcatccc cgacgctaat cacaataaat ggcttgccta aggtgatatt
    19021 tggcgcgtcg agttcgctaa gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact
    19081 gtgccagacc tgaccggtat agatgcctat cttgcgttct tccacattct gattgccatc
    19141 aaccatcaat gactcaggta accaaaaatg aataaaactt cgtttccgct ccacggtttt
    19201 atcgtcttcc tgcaccgtat aaagctcggt cacaggaata gcgatgagcg gcccaacgag
    19261 tttttgcggc ccgctggtac tttggcgaat cacatcttcc acatcgctac ggtaatcggc
    19321 acgttcgaca atcacctgcc gaatcagcat tatcggaatc aacaacagca atactgcacc
    19381 aaacaaggtg gtcattttcc agaacagggg ggatttcaac atagtcttct cctttgcaat
    19441 gacagcagga tacgaaggct atgtgggaag aatttgaagc tatgtgaagt gacggtgaag
    19501 tcgaagcgag gccagcacgc caccttcctg cacgttgcgc agcgtgactt cgccgttaaa
    19561 caaacgggcg acctcactga caaacgccaa ccccagaccg ctgctttttt gcccatttgc
    19621 acgcggcaaa gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag gaatcccact
    19681 accggtatcc agcaccttaa gggtgacgta ttcctgatcc acttcggcgc ttagcgttat
    19741 gcaaccgctc tcgggagtaa aatcgatggc gttatccagt aaattcccca gcgcctgctc
    19801 cagtaacgcc ggttcagaag caacgttaac ctcagtaggc ataacatgca aagtgatgtt
    19861 tttttctgcc aactgcacgg tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc
    19921 aacagcagtc agaacgactt cctgacgatt ctccagtctt gcctggcgta gtaacgtttc
    19981 caccagtgcc tgcattcgcg cattttgcgt cagaatgttg tcggtaaaac gagccaccac
    20041 ttccggcggc ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct
    20101 ttttagctca tgagttaacg cataaacata ctgctcaata tagtttttcc cttccagctt
    20161 cacgcgcata ctttccagcg cctgcgcgag tttacgcaac tcgctactac cgagatcggg
    20221 gagaggaacg ggcttattgt cagtgacgga atcagcatag cgagtgagcc tggcaataga
    20281 gcggttgatc caccaaacca tgcctgcgcc aatcaccagt gcaatcccca acaaaatggc
    20341 gctggcccat aaaattcgcc gctcgctacg cttaatgacc ggagccatcg ccgcgttcgg
    20401 tttgcctacg ctcaaaacgc caataagccg cgagccgtcc ataatcggcg cggcaacata
    20461 catcacagaa ctttcgggat cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc
    20521 acgcaacgtt agccagacgt cattccagcg cgaataatcc tgtccaacgg ctttatttgc
    20581 cgaatcgaac aatactttgc cctgcgcatc ggtcatatag acatgatatt cgttgcgcac
    20641 tttgttaatg ccaccgatat tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg
    20701 cgccagttgc ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca gctccgccag
    20761 caacgttgcg gtgtcgatta acgtcccctc ggttgctctt cgcacgcccg gtttaacttc
    20821 tttgacaaaa atagccagaa cgaaccaggc cgccaccgcc accagtaaaa aatagcccag
    20881 caacaaccgc atgccgatac gcattacagg cccctcaggc tatatcccat gccgcgatga
    20941 gtattaatcg gtgaaagatc ggggttgatg gcgcgcagct tggcacgcag cgttttaatg
    21001 tgggtatcga cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgct atccatcagt
    21061 tgctggcggg accagacgcg gcccggcgac ttgagtaacg tcttcagcaa taaaaactca
    21121 taccgcgtca gcgttaatgg cgtgtcaaac cagctgatct gcgccgcagg ttcattcaat
    21181 tcaaaatgac caatacggat aacgggagac ggcgtcgaga acttcttcac ccgacgcagt
    21241 aaggtgcgca ctctggcgca cacttcgcga ggtgaaaacg gtttagccac gtagtcgtca
    21301 gcgccaattt ccagcccaag caggcgatcg acctcttcac ttcgggccgt caggaacagt
    21361 acaggtaacg ccggatggag cgccagtaac tggcggcaca attcaaagcc gctgatatca
    21421 ggcaaaccaa catcgagaat catgacatcg ggaacctgct ggcgagcttt atccagcact
    21481 ggcaagcctc gctcaaagac ctcgacgtca aaaccttcct gttgcaacat gtagaccagc
    21541 gtatcggcta tcccttgctc gtcttcgact aaccagaccg tttcccgttg cataaatcgc
    21601 ctctgttatt gccgccacgg catgacagga accgcgctaa tcgcgttttt cggcgaacct
    21661 tctacaactt tgtcggagta agccagataa gcgagcgcgt tgcgtttagc gtcataaaag
    21721 cgcacgacct gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc
    21781 tgagctttgc cgtttttaat acgatccgac agttcaatcg gcccgacttg ttgacaagaa
    21841 atggccgcat cggaggtatc ttctgccagg cccaatcccc ctttaatacc accggttttc
    21901 gcccggctca cataacaggt gacatttttc acatcgggat cgtcaaaggc ttcaacaaca
    21961 attttgtgat ccgggccgat cattttaaat acggtgtcga ccgaaccaat ctcttctgca
    22021 tgagccaatg gccccagcat tatcaggctt aaagacagga ttaaatgctt gtatttcata
    22081 tcgttaccat ttttatctat tacacgagca attagttcgt cacggtaaat aacatattga
    22141 ttaagaacaa aatctcaata cttttatttc cgtcaagacc taaaacatac tcgactaaga
    22201 aaaaaacact gaatgctaaa acagcaaaaa atgctattat ccaattacct gatgtcaggt
    22261 gctcgttgtt gaaaggatga ggatatttta tggatcaggc cggcattatt cgcgaccttt
    22321 taatctggct ggaaggtcat ctggatcatc ccctgtcgct cgacaatgta gcggcgaaag
    22381 caggttattc caagtggcac ttacagagaa tgtttaaaga tgtcactggc catgctattg
    22441 gcgcgtatat tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc
    22501 gtccgattct ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca tttacccgcg
    22561 ctttcaagaa gcagtttgcc cagactcctg cactttaccg ccgttctcct gaatggagcg
    22621 cctttggtat tcgcccgccg ctgcgtctgg gtgaattcac tatgccagag cacaaatttg
    22681 tcaccctgga agatacgccg ctgattggtg ttacccagag ctactcctgt tcgctggagc
    22741 aaatctctga tttccgccat gaaatgcgct accagttctg gcacgatttt ctcggcaact
    22801 cgccaaccat tccaccagtg ctttacggtt tgaacgaaac acgtccgagc caggataaag
    22861 acgacgaaca agaggtgttc tacaccaccg cgttagctca ggatcaggca gatggctatg
    22921 tactgactgg gcatccggtg atgttgcagg gcggcgaata tgtgatgttt acctatgaag
    22981 gtctgggaac cggcgtgcag gagtttatcc tgacggtata cggaacgtgc atgccaatgc
    23041 ttaacttgac gcgccgtaaa ggtcaggata ttgagcgata ctacccggca gaagatgcca
    23101 aagcgggaga tcgcccaatt aatctgcgct gtgaactgct gattccgatc cgtcgttaac
    23161 gctgcagctc atctaatgca ggggcgtcca gatgcgagat gtcccctgcg gtttcaacca
    23221 cccagccaga cgccagccac aagctttcct gataatccac gcgcgaaata gaacagttac
    23281 gcagacgtaa gcggcgctct gcccatgctg gtaatccgag aatcgtactc accaggcatc
    23341 ccagtgcaat accatggctc accagtagcg gtcggcttcc ctgcggtaag tcccggcaag
    23401 attccagtgc cgcattaacg cgatcgctga gctcctgcat tgactcgcct tcaggaatac
    23461 gcccgtcaac ggtgccattg accagttgcc gacgccagtt ctcttcttct tcggtgagtg
    23521 aatcgatatt tcttgtttcc agtacaccca tatttaattc acgcaggcga gaatcaaaga
    23581 tgatgtcaca gccgcaggcc tgggcgatga tttccgccgt gcgccgggtc cgtcctaaat
    23641 cgctactgat gatatgcgta atgccaagct ctttggcacg ggttgccacc tgcatcgcct
    23701 gttgctcacc tttggcggtc agcgggctgt cagactggcc ctgaatacgt cgctcggcgt
    23761 tccactgcgt ttcaccgtgg cggactaggt atacctgtaa catgcttttt ttccgtatac
    23821 tgctgtaacg ttaattttct gagtaatgct gattatgcac caagttgtct gtgcgaccac
    23881 caatcccgct aaaattcagg ccattctgca ggcatttcac gagatcttcg gcgaaggatc
    23941 ctgccatatt gcatccgttg ccgtcgagag cggtgtaccg gaacagccct ttggcagtga
    24001 ggaaacgcgc gctggcgcac gaaatagggt agccaatgcc cgccgtttac ttcctgaggc
    24061 tgatttttgg gtggcgattg aagctggcat cgatggtgac agcactttca gttgggtggt
    24121 gatagaaaac gccagccagc gcggcgaggc gcgttcggct actttgccgt tgcctgcggt
    24181 gattctggaa aaagtgcgtg aaggtgaggt gcttggtccc gtgatgtcgc gttataccgg
    24241 tattgatgag attggtcgta aggaaggggc gatcggcgtg tttaccgccg ggaaactcac
    24301 tcgcaccagc gtctatcacc aggcggtgat cctggcgctg agtccgtttc ataatgccgt
    24361 gtatcagcca ttgcaggcct gattaggcgt tcacgccgca tccggcattc ggtgcacgat
    24421 gcctgatgcg ccatgtccta tcaggcctac aaaatcaatc gcttttcagc aacacgtctt
    24481 ccagccactg gcgcagctcg acgggcgcgg ctttcagact attagatcca cgcgtaatcg
    24541 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg ccgcgcaaca
    24601 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
    24661 ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
    24721 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
    24781 tagtatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
    24841 gagtacgata gcatatcata aacgtgtgga tcagtaacga cgtccccatt ccgtggcgct
    24901 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
    24961 tttcacataa ccgcgcgtct cggagaatgg aatactctcg acaaatgcca ctgcgtcgat
    25021 acgccctgcg ctgttgccaa gccaggttcg cacccgccct ggcccggcgt tataagctgc
    25081 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcaggt aactggtgcc
    25141 aatgttgatg tttgtttccg gatccagcaa ttgcccaggg ctgctataac cgggaataga
    25201 gaacatcttc accgtatggg tcgcggtacc aggcataatc tgcatcaggc cgctggcccc
    25261 taccggtgat ttcactttcg gattccaggc actctcctga cgggcaatcg ccatcgcata
    25321 gctttgcggg atctccttac cactggtgta gcgtttgaaa agatcgttgt aagccagcgg
    25381 gaatcgctct tccagatgat cccacagctt cccggcgatc gttgcctgaa cgctaagatc
    25441 ccaccattgg ttattgaaag cataccgagc cagttgagcc tgctctgttt ttgacttgct
    25501 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
    25561 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
    25621 atcaatcttc agctcatact cttcgccgat gcgttgtgca gcaaccatcg ggtagaaacc
    25681 acgctgttgc atgagttgat gcaaaatctc ttttgcttca gcttcacgtc cgcgttccag
    25741 caataaatcc gcctgccagt aacgccattc atctttctct ttcgcttcca taggcagacg
    25801 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agcgccattc gcacacggcg
    25861 ttcaataagc gaagtagatt gcgaacgcat aatcgcgtca tcgcgccatt tcgcctgctc
    25921 gtcggtgaca tcgttgccca tcaaacgcca tgcgacaata tcgcgcagct cctgaatctg
    25981 atcttcatta agctgctgtg cctgagcaag cgacgggatc atcagccgtg cattttcagc
    26041 atcctgccgc gccacactgg caaacgccac cgccgccatt tgacgggtaa aatcggtcgc
    26101 gccggttgta cgcgcgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
    26161 cgaagcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccggt
    26221 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
    26281 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
    26341 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
    26401 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
    26461 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
    26521 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
    26581 gtcatcagtg atctggcggt attccaggta gggataaagt gggtaatctt tcagtccagg
    26641 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
    26701 acgactacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
    26761 gacaccgaca gccaacagcc gccaggtaac gtgtttggct ttttccacaa gcgcatcctc
    26821 taagtgtcaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    26881 tgccgtttac gccacgttac ggacactttt ttacttttac ggggtgatcg actggctggg
    26941 attagacagg gaaacaggct acacttcgcc tttgaaaact catcatcacg ataaacatag
    27001 aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc
    27061 cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg
    27121 gtgtcctggg tctgaacggc gcgggtaagt ccaccctgct gcgcattatg gcgggcattg
    27181 ataaagacat cgaaggtgaa gcacgtccgc agccagacat caagattggt tatctgccgc
    27241 aggaaccgca gctgaacccg gaacacaccg ttcgtgagtc cattgaagaa gcggtttctg
    27301 aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtatgcc gatccggatg
    27361 ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg
    27421 acggtcacaa cctgaacgtt cagctggagc gtgcggcgga tgcgctgcgt ctgccggact
    27481 gggacgcgaa aatcgctaac ctttccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc
    27541 tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgcgg
    27601 aatccgtagc gtggctggaa cgcttcctgc acgacttcga aggcaccgtg gtggcgatta
    27661 cccacgaccg ttacttcctc gataacgttg cgggctggat cctcgaactt gaccgcggtg
    27721 aaggtattcc gtgggaaggt aactactcct cctggctgga gcagaaagat cagcgtctgg
    27781 cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg
    27841 tacgtcaggg aactaaaggc cgtcagtcaa aaggtaaagc acgtctggcg cgctttgaag
    27901 aactcaacag caccgaatat cagaaacgta acgaaaccaa cgaactgttt attccacctg
    27961 gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tatggtgatc
    28021 gtctgctgat tgactccctg agcttctcga tcccgaaagg agcgatcgtc gggatcatcg
    28081 gtccgaacgg tgcgggtaaa tcgacgctgt tccgtatgat ctctggtcag gaacagccgg
    28141 acagcggcac catcactttg ggtgaaacgg tgaaactggc gtcggttgat cagttccgtg
    28201 actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg gatatcatga
    28261 agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg
    28321 ttgatcaggg taaacgcgtt ggcgaactct ccggcggtga gcgcggtcgt ctgcatctgg
    28381 cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg
    28441 atatcgaaac cctgcgcgca ctggaaaacg ccctgctgga gttcccgggc tgcgcgatgg
    28501 ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac ccacattctg gattaccagg
    28561 atgaaggtaa agttgagttc ttcgaaggta actttaccga gtacgaagag tacaagaaac
    28621 gcacgctggg cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt attgcgaagt
    28681 aatgcgtaaa atgccggatg cggcgcacca gcatcgcatc cggtattagt taaatgatta
    28741 atgggaagca agttcccgga aaaaggcagg attttcttta attgtggcaa gtacttttgc
    28801 agccaatccc gtaggatttc ttcgcttcga ttcccagctt ttaatggtat ccacactggt
    28861 tcccagagct tttgccatct cactttgaga aacgttgagt tgctcacgaa tagctttaac
    28921 atccgcaatt tcgtagcggg tcacccgtgc aggttctttt aaaccttgtt taatctcgac
    28981 agcctcttcc agagaggttt tcaactcatc aaaaaaactc attttatatc tcgcctttga
    29041 gcagtttcgt caacttcttt agttgagctt tttccgtatc tgtcagacta tctttggcat
    29101 ttttcggata ggccataatc aaataaataa tctcttccgt cgccagaaag taaattattc
    29161 tgacactgcc actttttccc tgtgaacccg cagccattcg gactttacgc aatccaccag
    29221 tctgctgaat gagatcacct ttatcgggag ttccgatgag ttctttttgt aattccttta
    29281 attcatcatc ggttgcaatc tgcttgatct gacgcgtaaa cattggcgtc tctataaata
    29341 ttatcgcctt acccattccc cctccctggc aggtacattg tacaccgaaa tggagtacta
    29401 agtacacttt tgaatcagta atcccactca acatcttata aagatgatta tctttgctcc
    29461 cccatcatct cccgcaccag ctccacgcag cgcaggaaac ggctgtcgta atcgtcctct
    29521 tcaacccgca cgaattcgat attgttctct tccagcatct ccaccagaaa gttctggaac
    29581 tctttgcgat ccaccgaact gccgaggctg cgtaaaccat ccgccaccca cggcgtgttg
    29641 ttctccagca ggatcaccag atcgaaacgg tactcatcaa tcaacgcctg cacgaacgga
    29701 tgctcacgcc cttcgtactt tttgcagaac gcctgggtag tgacaaaatc ggtgtcgata
    29761 aacgccactt tattggcata tttcactgca aaatcaatat attgcgcatg tcccagcgcg
    29821 attttatcgt agtcggaata ctgcaacgcg atctcatcgc caccgaggtg cgaaaagaca
    29881 taatcacgac catattccca cgcactggta gtgttgaaga tattggcaag tttgtttacc
    29941 agggtggatt taccgctcga ttcaccaccg aggatcgcca cggtacgcac aaagaatggc
    30001 ttcacttccg taggaatata ttcccagtag cggaacgggt tttcgcggat ctgcgcaccg
    30061 ctgatactca taaaggtacg cttcggatcg accagcaccg tctcgatccc cagatgttcc
    30121 atatactgcg gcgcatcggc ttcttccgag gtgtagatca ggtccggctg aatgcctttt
    30181 tcggccataa actttttgat gccgttgctc cacacatccc agccgtgtgg atacggttcc
    30241 atgccctctt cgttaaaagc atgaatgcgg atattttttt gatacttaaa ggtctgcaat
    30301 aaccagcgca gacgatccgg cacggttggc tgctgcgaca tggcgctgtc ttcgaacaat
    30361 gcgcggtcgc gggtatcgtc aaaacccata atgatatgca gctcgtcaac ctggctacag
    30421 gcgcgctgga taaggtagat atgtccagta tgcagcgggt aaaacttacc gaatacgaca
    30481 ccgatcgttt tcttctgccg gggaaattca agtcccaaat aacggtgcaa tgcctccagc
    30541 ttttgtgcgc tggggctttt aattttggca ttcagtaact ggcttaaata ccctttggtc
    30601 ataccgctgg catcagctac ctgctgtaga gtgcagccct gttgcttgat ggcagttttc
    30661 aggtaatcaa atgacgacat atctccctcc gtatctctca ttataagtcg tcgaacacgc
    30721 taagcgcgtc ggagagtttt ttaacgccaa aaatctgcat cccttccggc gcttttttcg
    30781 gcacgttagc tgccggaaca attgcccggc gaaaaccgtg tttcgccgct tctgagattc
    30841 gttcctggcc gctgggcacc gggcggatct cccctgccag cccgacttca ccaaacacca
    30901 ccagatcctg cggtagcggt ctgtcacgca ggctggaaac catcgccagc agtaacgcta
    30961 aatcggcact ggtttccgtc accttcacgc caccgaccac gttaacaaac acatcctgat
    31021 cggccatttg tagaccaccg tgacggtgaa gcaccgccag caggattgcc agacggtttt
    31081 gctccagccc gactgccacg cggcgtggat tcgccatcat cgagtgatcg acgagcgcct
    31141 gaatctccac cagcagcgga cgcgttcctt cccacactac catcactgag ctaccggagg
    31201 tcacttcatc gccgcgactt aagaaaattg ccgaagggtt gctgacttca cgcagcccct
    31261 gctcggtcat tgcgaagaca cccagctcat tcaccgcgcc gaagcggttt ttatggctgc
    31321 gtaaggtgcg aaaacgtgag tcggcatcac catccaaaag caccgaacag tcgatacagt
    31381 gttccagcac tttagggcca gccagcgagc catctttggt tacgtgcccg accatgacaa
    31441 tcgccacacc gcgcgttttg gcgaagcgcg tcagataagc cgccgtttca cgcacctgtg
    31501 ccacgctgcc aggcgatgat tgtacatccg ccatatgcat cacctggatg gagtcaatta
    31561 ccatcagctt cggttgctct tcttcggcaa tcaggcagat ctgctcgatg ctggtttccg
    31621 acagcatatt aagattgtca gtcggcaggc caaggcgatg agcgcgcatt gccacctgtt
    31681 gcagcgactc ttcgccggtg acatacagcg ttttcatctg ctgcgccagt ttgcacagcg
    31741 tttgcagcaa cagcgtggat ttccccgcac cagggttacc gccaatcaga atggcgcttc
    31801 ccggcaccac gccgccgcct aatacgcggt cgaactcttt aaatccggtg gaaaaacgcg
    31861 gcagctcttc aaggctgatg tcggaaagtt tctggacttt tgccactccg gcgctaccgg
    31921 catagccact gagacgctcg ttacgcgcca ccgttggcga cgctgcaaga cgcacctcgg
    31981 tgatggtgtt ccaggcatga caggcactgc actgcccctg ccagcgcgga taatcggccc
    32041 cgcattcatt acaaacaaag gcgcgttttg gagcttttgc cacgatgtac ctcgttaatg
    32101 ctgtgccgcc cgcaggatga cgggcggaca attacttctg attcaggctg ccagagagga
    32161 tgcagaatac ccccatcagg tcagcgtgac ggatggtgac ttccgtcttt tcattcactt
    32221 ttggcttggc atggtaggca atccccagcc ctgccgcttt gatcatcggc aggtcattgg
    32281 ctccatcgcc aatcgccacg gtctgcgcca gcgggatttc atactcctgc gcgaggcggg
    32341 tcagggtttt cgctttatat tgcgcgtcaa cgatgtcgcc gatcacattg ccggtaaatt
    32401 taccgtccat gatctccagt tcattggcta ccacggctgt caggcgcagc ttgtcgcgca
    32461 ggtattcagc aaagaaagta aagccgccgg aggcaatcgc cactttccag cccagcgttt
    32521 ccagcttgag taccagctgc gttaagcctg gcatcagcgg cagattttca cgcacctgtt
    32581 gcagaatatt ggcgtcagcg cctttcagcg tcgccacgcg gctgcgcagg ctggcggtaa
    32641 aatcgagttc gccgcgcatc gcccgttcgg ttacctccgc caccctctcg cccgttccgg
    32701 ccagtttggc aatttcatca atacattcaa tctggatggc ggtggaatcc atgtccatca
    32761 ccagcaaccc cggcgtgcgc aggtgtggga ttttccccag cggcgcgaca tccagttgcg
    32821 cttcatgggc caggcgcgta gcccgcgcag tgagtgaacc tgccagacga atcacctgat
    32881 aatcttccac gcaccaggcg gcaacaatca ccatagccgc gcccagtttg ctctggtatt
    32941 gggtcagacg ttgtttatcc agcccacgac catacagcag ccagccgcta cgacctgcgt
    33001 ggtaatccag tggcatcact tcatcaccac ttaatgaaag aggcagacca ggccataaag
    33061 agacatcttc aggcaggtcg caccaggtaa tgttaggcat taaggctcct gtaaaatcgt
    33121 tcgaagcagg gaaaataacg catgaggcta ccttgtatcc attgcttctg gcaacattaa
    33181 gtctcaaatt ttcaaagggt ggaagatggc tcgcacaaaa ctgaaattcc ggctgcatcg
    33241 ggcagtgatt gtcctgttct gtcttgcctt gttagtggcg ctgatgcagg gggcgtcatg
    33301 gtttagtcaa aaccaccagc gacagcgtaa tccgcaactg gaagaactgg cccgcaccct
    33361 ggcgcggcag gtgacgctaa acgttgcacc gctgatgcgt accgactcac cggatgaaaa
    33421 acgcattcag gcgatcctcg atcagttaac ggatgaaagc cgcattctcg acgcaggcgt
    33481 atatgatgag caaggcgatc ttatcgcccg ttctggcgaa agcgtcgacg tgcgcgaccg
    33541 gctggcgctc gacggcaaaa aagcgggcgg ctattttaac cagcagattg tcgaacccat
    33601 tgcgggtaaa aacggaccgc tcggctatct gcgcctgacg ctcgacaccc atacgctcgc
    33661 caccgaagcc caacaggtgg ataacaccac taacatttta cgcctgatgt tgctgctctc
    33721 gctggcaatc ggcgtagtgc tgacccgcac gctgctacag ggtaaacgca cccgctggca
    33781 gcaatcgccc ttcctgttaa ccgccagtaa accggtgccg gaagaggaag aaagcgagaa
    33841 aaaagagtga ctcattacta caagaaagga aatcgttatg tccacattac gcctgctcat
    33901 ctctgactct tacgacccgt ggtttaacct ggcggtagag gagtgtattt ttcgccagat
    33961 gcccgccacg cagcgcgttc tgtttctctg gcgcaatgcc gacacggtag tgattggtcg
    34021 cgcgcagaac ccgtggaaag agtgtaatac ccggcggatg gaagaagata acgtccgcct
    34081 ggcgcggcgc agtagcggtg gcggcgcagt gttccacgat ctcggcaata cctgttttac
    34141 ctttatggct ggcaagccgg agtacgataa aaccatctcc acgtcgattg tgctcaatgc
    34201 gctgaacgcg ctcggcgtca gcgccgaagc ttccgggcgt aacgatctgg tggtgaaaac
    34261 cgccgaaggc gatcgcaaag tctcaggctc ggcctatcgc gaaaccaaag atcgtggctt
    34321 ccaccacggc actttgctgc tcaattccga ccttagccgc ctggcaaact atctcaatcc
    34381 ggataaaaag aaactggcgg cgaaaggcat tacctcagtg cgttcccgcg tgaccaatct
    34441 gaccgaattg ctaccgggga tcacccatga gcaggtttgc gaggccataa ccgaggcctt
    34501 tttcgcccat tatggcgagc gcgtggaagc ggaaatcatc tccccggaca aaacgccaga
    34561 cttgccaaac ttcaccgaaa cctttgcccg ccagagtagc tgggaatgga acttcggtca
    34621 ggctccggca ttctcgcatc tgctggatga acgctttacc tggggcggcg tggaactgca
    34681 tttcgacgtt gaaaaaggcc atatcacccg cgcccaggtg tttaccgaca gcctcaaccc
    34741 cgcgccgctg gaagccctcg ccgggcgact gcaaggctgc ctgtaccgcg cggatatgct
    34801 gcaacaggag tgcgaagcgc tgctggttga cttcccggaa caggaaaaag agctacggga
    34861 gttgtcagcg tggatagcgg gggcggtgcg atagttaccc gcccatgcgg gcaactttcc
    34921 cttcgatttg ccggattttt tccggcattt cttgtgcaat gtggcgaaat gcctcgctga
    34981 tacgcggatc tccggcgacc gtttgccaga actgctgcgc cagcgggatc gccgttaacc
    35041 atgcactttt actgacgtta agatcaaacc tcacctcaat ggcagcatca cgcaacattc
    35101 cggcgctgtt tggtgcatag gccatcggca gcatgtcgta gacgggcgtc agcgcaaatg
    35161 gtggttcaga taaataaaat gataaattgc ctgcgtgcat atcgctgttg gcgataagtc
    35221 gcccaaacgc ccagatcact tctgtttgcg ccacgctctg gtgagtgaca agttgttgct
    35281 cacacaaacg gcgcatagcc tccggccacg atcccggaga agagataaac tcactctgca
    35341 ccgcctccag cgacacaatg ggcaagcgac catcgttacc tttgcagtca aatcgttccg
    35401 cttcaaggaa tacctgcctg ttacttgtta caagcaccgt tgactcgatg gcgtggatcc
    35461 cgccgtcgcg caggatttgc gcggcaatag attccgcaat aagcaagtcg ccccaacgtt
    35521 ggctgaccgc ggtttgctgt ggcacggtga atttaaccag cacatgttta ttgcctgacg
    35581 gcgtttgtgc atagcaggta aattttggct gctcaccgcc cgcagaagaa cccacaattt
    35641 ctcctgccag tgcatcgctt gccagttgtt cgtaatgggt gagtttttga tccagaggaa
    35701 ttgccgcagg gcgttgtgca gtaatccatc gctgataatt cccctccccg accagccagc
    35761 cgccggtgta ttcaccgcta aatacggtca gggcgtagag cacatcttct tcctgccaga
    35821 gacggatatc ttcagtaaga ttcagttgcg cggctaactt cctgccccac gcgcgcccca
    35881 aaaacccctg cggtcgcaga tcggtcaaat accagggcaa accatcaaac caccgttcgt
    35941 cgccatctgc gcctgttacc agacaacttc cctgcggcca gcacaaccgg atgtcggcga
    36001 atttatgcgc ctttccggca tcatccactc gccatacggg aatacgctca attccgcgat
    36061 aaggacgcag cagtgcatat cgcgttgccc gtgctttacc aaagcgaatc acccgatctt
    36121 ctctggcaac aaggcgtgag aacgtcgcct gactgattgc cagacgctgg cgcaattccg
    36181 gggcagaacg cggcccttgc aataaaagat cggtcaattc gctcattaat gaatagattc
    36241 gtgaatagat acttgaatag atttattaac acatttcact gaaaagtata gcgaaaatat
    36301 tcaagctgga gagagaaatg aatagataat aaaaagccgg agcagtctcc cactccggct
    36361 tcacacgaca atcgcctttc agcaaaacac aattactctt tatcgcccag cagaacggat
    36421 tccagcgcga ttttgatcat gtcgttgaag gtggtctgac gctcagcggc agtggtctgc
    36481 tcgtgagtgc ggatgtggtc agacacggtg cagatggtca gggctttcgc gccaaattct
    36541 gcagcgacgc cgtagatacc agccgcttcc atttccacgc cgaggatgcc gtatttttcc
    36601 atcacgtcga acatttcgcc gtccggagag tagaacaggt cagcggagaa caggttacca
    36661 acgcgagcat caacgcccag tgcttttgcc gcgtctaccg cgttacgcac catgtcgaag
    36721 tcagcgatag cggcaaagtc atggtcttta aaacggatgc ggttaacttt ggaatcggtg
    36781 caggcaccca taccgataac gacgtcgcgc agttttacgt gcggcagaac agcgccacag
    36841 gaacccacgc ggataatttt cttcacgccg aaatcagtga tcagttcttt ggtgtagatg
    36901 gagcaggacg ggatacccat accgtggccc attacggaaa ttttgcgacc tttgtaagta
    36961 ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac gggcatcttc aaggaaagtt
    37021 tcagcaatat acttcgcacg cagcgggtcg cctggcatca aaactacgtc agcaaaatcg
    37081 cccatttctg cattaatgtg tggagtagcc atcgttttat cctttttgtg acatattaaa
    37141 ggcggagtca ctgctccgcc caaatccatc agaacatggc tttgccatat tccatatcag
    37201 aagtaccaaa atattttgcc agagtctggc cgatatccgc gaaggtttca cgatgcccca
    37261 gcgaacctgg tttcactttc gggccgtaaa ccagtaccgg aatgtgttca cgcgtgtggt
    37321 cagtaccggt ccaggtcggg tcacaaccgt ggtcagcagt gagaatcaga atatcgtcat
    37381 cgcgcagcaa agacatcagc tccggcagac ggcggtcgaa cagttccaga cccgcagcat
    37441 aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa gtcaacgaag ttggtgaaga
    37501 cgatggtgtt atcacccgct tctttcatct ctttgatggt ggcgtcaaac agcgcgtcca
    37561 ggccggtcgc tttcactttt ttggtgatac cgcagttggc gtagatgtct gcaattttac
    37621 cgaccgaaac cacctggccg tgtttttcat caaccagttt ctgcagcacg gtcggtgctg
    37681 gcggctcaac agccaggtcg tgacggttac cagtacgctg gaagttacca gctttgtcgc
    37741 cgataaacgg acgagcgata acacgaccga tgttgtagcc gccgttggtc agctcttcac
    37801 gagcgatttc gcacagttcg tagagtttgt ccagaccgaa agtttcttca tggcaggcaa
    37861 tctggaacac ggagtcagcg gaggtataga aaatcggctt gccggttttc atgtgctctt
    37921 cgcccagctg atccagaatg accgtaccgg aagagtggca gttaccgagg taacccggca
    37981 gattagcgcg ttcaaccagt ttatccagca gctctttcgg gaagctgttt tcgtgatcgg
    38041 agaagtatcc ccactcaaac agaaccggga caccggcgat ttcccagtga ccagacgggg
    38101 tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc gccgataact tcagcgttgc
    38161 cgtccattcc cgccggaatg aaaccggtag aaccttcgtg tgctttcgcc agccccagac
    38221 gggtcagatt tggcagattg agcgggcctt tacgaccgtt atcagcttcg cctttggcac
    38281 aagcttctgc gatatgaccc agggtgtcag ccccgacgtc accaaagcgt tctgcatctt
    38341 ctgtagcgcc gatgccgaat gagtccagca ccataataaa tgcacgtttc atatgttctc
    38401 cgtactcagt gcttcaaatt taaatgatca gatcagtata ccgttattcg ctgatacggc
    38461 gatagacagt tggtgtgctt tccggtgctt tatcggcaag tttaattgcc gctttcaccg
    38521 ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgtg gataactgcc agcggacgct
    38581 gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa gccgacgctg taatcgatgg
    38641 tgtcagatgc ctgacggcgt ccgccgccca ttgcaaccac tgccatcccc agcgcgcggg
    38701 tatccatttc actgacaaac ccttcggtat cagcatagac tgctttcgtc agcatcgctg
    38761 tcggcagata cttcgcgtag ttctcaacga agtcggtcgg acctttttgt gccgctacca
    38821 tacgaccaaa gacttctgcc gctttaccgt tgtccagcac cgcctgcaat ttcgcgcgcg
    38881 cttcggcgtc atttttcgcc agtttgccgg agatcagcat ctccacgcac agtgccatcg
    38941 tgacatcaaa cagacgcggg ttacgatatt cacccgtcag gaactgcacc gcttcacgaa
    39001 cttcaaccgc gttacctgca ctggaggcca gtacctgatt catatcggtg agcagcgcgg
    39061 tggtgcgcac gccagcgccg ttagccacgc caacaatcgc ttcggcaagg gcttcagaga
    39121 gttcgtaggt cggcataaac gcgccgctac ccactttcac gtccatcacc agcgcgtcca
    39181 gaccttccgc cagtttcttc gccagaatag aggcggtgat cagcgggatt gaatccacgg
    39241 ttgcggtaat atcgcgggtc gcgtagaaac gtttatccgc cggagccagt gagctggtct
    39301 ggccgataat cgccacgccg acgtctttaa taatttcgcg gaaacggttg tcatccggga
    39361 aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt accgccagta tgaccgaggc
    39421 cgcgaccgga gatcatcgga atatagccgc cacaggcagc gaccatcggc cccaacatca
    39481 gcgaagtcac atcgccgaca ccgccggtcg agtgtttatc aacaatcggg ccattcagat
    39541 gcaggctttt ccagtcgaga acggttcctg aatctcgcat cgccatggtc agcgagacac
    39601 gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc gagggcggca atctgccctt
    39661 cggagatagt gttgtcgcga atgccgttga taaagaaacg aatttcttca tcactcagcg
    39721 catgaccatc acgtttttta cgaataattt cttgtgcgag aaacaaggta acctcccgtg
    39781 gaaaagagac gaggtaatcg gggaaccgga tgagcatcgc tgcatcatcc ggcaaatcaa
    39841 gccttagtag ctgctggcgc tcttaccgtc gccgtggccc agagctttca gcaggcttgc
    39901 cagcaagctg gaagcaccaa agcggtagtg acgcgcatca gcccagtcag caccgaacag
    39961 ttcatctgca atggcgagat atttctgcgc atcttccgca gtacgcacgc cgcccgccgg
    40021 tttgaaacca acggtttttt ctacgcccat atcgcggatc acttccatca tgatgcgcgc
    40081 gctttccggc gtcgcattca cagccacttt accggtagag gttttgatga agtcagcacc
    40141 cgctttgatg gagatttcag acgctttacg gatcagcgct tcgtctttca gttcgccggt
    40201 ttcaataatc actttcagca gtacgttcgc cgcagcgcaa gcctctttac aggctttcac
    40261 caggtcaaaa ccaacctgct cgttacctgc catcagcgca cgatacggga acactacgtc
    40321 aacttcatcg gcaccgtagg cgattgccgc acgggtttct gccagcgcga tttcgatgtc
    40381 atcgttaccg tgtgggaagt tagttaccgt agcgatacgg atttccgggg tgccctgctc
    40441 tttcagcgtt ttgcgagcaa ttgggataaa gcgaggatag atacagatag cggcggtatt
    40501 gccgaccgga gttttggcct gatgacacag ggcgatcact ttctcatcgg tgtcgtcgtc
    40561 attcagggtg gtcaggtcca tcaatttcag tgcacgcagg ctgcttgctt tcagatcagt
    40621 catttcattc tcctggcttg tcgccaataa aattcacctt gcgagtttgt tagtattcta
    40681 acatctactc cgcaacacac ttcgatgtac atcacaatta aggaaatcta cttacaagtt
    40741 tgcatcactg taatgcgatc tggttcaaat aattcacttt caaatgaatg cgtcagtggt
    40801 ggcaaacgta tcaggatcaa agtgaacatc acgaaacttc ttacaatggc gcatcacctt
    40861 cggcataaaa ggaaaagaag atgcccactt ctcatgaaaa tgcactgcaa caacgttgcc
    40921 agcaaattgt caccagcccg gtacttagcc cggagcagaa gcgccatttt ctggcactgg
    40981 aagcagaaaa taatctgcct tacccacagc tgcctgccga agcccgccgc gcgctggatg
    41041 agggggtaat ctgcgatatg tttgaaggtc atgcgccgta caaaccgcgc tatgtcttac
    41101 ccgattacgc ccgttttttg gccaacggtt ccgaatggct ggagctggaa ggcgcaaaag
    41161 atcttgatga cgcactctct ctgctgacca ttctttacca ccacgttcct tcggtcacat
    41221 cgatgccggt ctacctgggg caactggatg cgttgttgca accgtatgtt agaattctaa
    41281 cacaagacga gatcgatatt cgaataaaac gtttctggcg ttacctcgac agaaccctgc
    41341 cagacgcctt tatgcatgcc aatatcggcc cgtctgattc gcccattacc cgtgcgatat
    41401 tacgtgcaga cgcagagctg aagcaggttt caccgaacct gacctttatc tacgatcctg
    41461 atatcacccc tgatgacctg ctgctggaag tggcgaaaaa catctgtgaa tgtagcaaac
    41521 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    41581 tgagctgtta caattcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    41641 tgaaagccat tgccgagcgc agtgaatcgc tggaggactt ctttacgcgc actctaccgc
    41701 actactgcca acagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    41761 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    41821 tcccgatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gaaaaagaag
    41881 gaattgccgc gcgttacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    41941 cgcaactggc ggagtttgtc gccaatactc ctgtgaaata tggctggcaa aaacgcgcca
    42001 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttac
    42061 catatggcga tgagccagat ccgattaccc atctgcaaac tgtcgccccg catcatgctt
    42121 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaatccgc
    42181 aggcgctggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    42241 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    42301 aaaaatatcg cgccgaaggt tcacgcacca acactacctg gctgggagaa gaagccgcac
    42361 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    42421 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    42481 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatggggcgc
    42541 tgtaatgatt gcggagagtg cgtgccgcag tgtccgcatc aggcgttgca gattgtcgac
    42601 ggcaaagtgg cgtggagcgc ggctgtctgc gagcaatgtg atacctgcct gaagatgtgt
    42661 ccgcaacatg ctacgccaat ggcgcaatcc atgagcgtgg acgaggtgct taaccatgtc
    42721 cgcaaagcgg ttctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccag
    42781 ttgccgtttg tggtggcgct gtttactgcc atcaaaaacg atccgcaact gtgccatctc
    42841 acctgcctcg tggacagtaa cggcatgttg agcgaaaccg gctgggaaaa attgctcccg
    42901 gtgtgtgatg gcgcaatgct cgatctcaaa gcgtggggga gcgaatgtca tcagcaatta
    42961 accggacgcg ataatcagca gatcaagcgc agcatctgtt tgctggcaga gcgcggtaag
    43021 ctggcggaac tgcgtttgct ggtaatccct tgccaggtgg attatttgca acacatcgaa
    43081 gaactggctg cgttaatcaa gggacttggc gatgttccgg tacgcctgaa cgcgtttcat
    43141 gcccacggcg tgtatggcga ggcgcaaagc tggccgagcg ccacgccgga ggatgtggaa
    43201 caactggctg atgcgttacg ggagcgcggg gtgagccggt taatatttcc ggcgctatat
    43261 ttgtgacatg ccggatgtgg cctacggatc ggcacagacc tgtaggcatg ataagacgcg
    43321 tcaagcgtcg catcaggcat tgtgcgccag ctgccggatg cggcgtgaac gccttatccg
    43381 gcctacggtt cggcacagac ttgtaggcat gataagacgc gtcagcgtcg catcaggcat
    43441 tgtgcgccaa cggccggatg cggcgtaaac gccttgtccg gcctacggca cgttaaacaa
    43501 cgtataggtg ttattaagca acacttcggc aatctcatcc gccggttccg gtcgtaactc
    43561 gcaaagcacg gcaaacacac gggcggcctg ttcagggcga ttaggctgcc cctgaaaacc
    43621 attgagcggc atatctggtg cgtcggtttc cagcaacaac gatgccagcg gtaattttgc
    43681 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtcccgccta cgccaatttt
    43741 gtagcccagt tgcacaaacc gttcggcctg ttgcaggctg ccggaaaaac catgaaccac
    43801 gccagtgcac ggtaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgtcg
    43861 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    43921 ccactgctgc ctctcaaatt gcggatcgtc gccaaagaga tccagaccga tctcccccac
    43981 cgccaccacc ttcgccggat gcctttccag cgcctgctgt agctgatcaa gagacacatc
    44041 gctatgtttt tccaacatac caggatgcaa gcccaatgcg gcatacagcg gttgatattt
    44101 ttccgctaac gcctgcacgc gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    44161 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccac taaacggcgg
    44221 gaaatcaaaa tggcagtggg tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    44281 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    44341 actggcgcag gcggaataac gatcgactcc ggcgtcacca ccggcaccag atggcggctc
    44401 agcggcgctt tttcagtcaa taatttaccg accgttgcca gaaaataacg cccgcataaa
    44461 cgtccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccgagcgc gatactatgc
    44521 aatggcttcg gcggataaat ttcgaatatc cgcagctggc ccggtggttt ctcaataaat
    44581 tgctgaatct cacgatagct tgtttcatga tgctgcacca gattgaccag cggctgcagg
    44641 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    44701 gacggcacag tgcggatgac gaccaacgtt tttgctccct gccttgccgc ctctttaacc
    44761 gggatcgcat cactgatccc accatccagg tagttaatac cttccagcga cactccgcta
    44821 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttagtt
    44881 ggcaaaaagt aattcggcgc atagtcatcc tgacgacagg cgcacatata aaacgatttg
    44941 ccgctgtcaa ataaccgcgc tgcggtatcc atttgcaacg gcatctggct tgcggtggcc
    45001 tccaccagcc aatcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    45061 aattcgcgtt ttgtggtgta acgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    45121 atatacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    45181 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ttgtccgccg
    45241 ccttcgcaca ccagagcaat ccgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    45301 ttaccaagcg ttacaggtat tcgctgcccc acctctgcct tcctgttttt gttgtgaaag
    45361 aatgacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    45421 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    45481 actcaccagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcagt
    45541 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    45601 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    45661 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    45721 gggaggtcga attacgacga attacttagt tttcagatca tttttaacgc ttttcacacc
    45781 atctaccgct ttggcgatac tttcagcacg gtcgctctgt gcctgagaat ctacggtacc
    45841 ggagagctgg accacgccat cggtggtttc aactttcaca tgacgggaag ggacgatatc
    45901 gtccgccagc agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt
    45961 cacagagcct tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccctc
    46021 aacacctttc gccactttca ccgcctcttc ggcctgagcc tggctttcaa cgaaaccgct
    46081 cagggtcacg actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg
    46141 atccaccagg gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac
    46201 tttattcata gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt
    46261 ttctgcgtag gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt
    46321 cgaaatcttc agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga
    46381 gcctggcaac acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca
    46441 atggtcgcca tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag
    46501 ttattaatat cggtagatat gacaatcgac ggctttagat actgatatct acgcattgaa
    46561 cggtatttaa cgccgtcaga aatgtcatta ctttgttaaa tatagatcac aattttgaaa
    46621 ccgctcggga tatcagctaa aacataagca aaagtgaatg ttttaagaac attccgtaag
    46681 cggctaataa ggaagggaaa ttgacagggc gcagcggcta ccgcgccctg gaggctagaa
    46741 ttaatgctcg cgggtctggt ggaactgaac gtccggataa cgttcctgcg ccaggcgcag
    46801 attgaccatg ctggtagcga tgtaagcgag gttatcgccg ccatcaagcg ccagttggct
    46861 ttcgttctta cgcttgaact cttcgaattt cttcgcgtcc gcacattcta cccagcgggc
    46921 ggtggcaacg ttgactgact catacactgc ttcaacgttg tattcgctct tcaggcgcgc
    46981 taccaccaca tcaaactgca gcacaccaac cgcgccaacg atcagatcgt tgttggagat
    47041 cggacggaac acctgcaccg ccccctcttc ggaaagctgt accagccctt tgagcagctg
    47101 tttttgcttc agcggatctt tcaggcggat acgacggaac agttctggcg cgaagttcgg
    47161 aataccggtg aacttcatca tctcaccctg ggtaaaggta tcgccgatct gaatggtgcc
    47221 gtgattgtgc aggccgagga tatcgcccgg atacgcttct tcaacgtgcg aacggtcgcc
    47281 tgccataaag gtcagcgcgt cggagatcac tacatctttc gcggtgcgca cctggcgcag
    47341 cttcatgcct ttttcatatt taccggatac cacgcgcata aacgccacgc ggtcgcgatg
    47401 tttcgggtcc atgttggcct gaattttaaa tacgaagccg gtaaatttat cttcgctcgc
    47461 ttctacggta cgggtatcgg tctgacgcgg catcggcgca ggcgcccact ccaccaggcc
    47521 atccagcata tgatcgacgc cgaagttacc cagcgcagta ccaaagaata ccggggtgat
    47581 ttcgcccgca aggaacagct ctttgtcgaa ctcattagac gcgcctttca ccagctccag
    47641 ttcgtcacgc agctgctgtg ccagatcttc accaaccgca gcgtcgagat ccgggttatt
    47701 cagcccttta acaatgcgga cttcctgaat ggtgtggcct ttaccgctct gatagagata
    47761 ggtttcatct ttataaaggt ggtaaacgcc tttaaacagc ttgccgcagc caatcggcca
    47821 ggtgatcggt gcgcagccga ttttcagctc gttctcaact tcatcgagca gttccatcgg
    47881 gtcgcggata tcacggtcaa gtttgttcat aaaggtgagg atcggcgtat cgcgcagacg
    47941 ggtaacttcc atcagcttac gggtacgatc ttctacacct tttgcggcgt cgataaccat
    48001 caggcagcag tccaccgccg tcagggtacg ataggtatct tccgagaagt cttcgtgccc
    48061 cggggtatcg agcaagttaa ccaggcagtc gtgatacgga aactgcatca cagacgtagt
    48121 gatggagatc ccacgctgct tttccatctc catccagtcc gacttagcgt gctggttaga
    48181 gccacggcct tttactgtac cggcggtctg aatggcctgt ccgaacagca gcaccttctc
    48241 ggtgatggta gtcttaccgg cgtccgggtg agaaataatg gcaaaagtgc ggcgcttcgc
    48301 cacctcttgc aaataaggag acaacgtcat aattctttct tcttgagtaa gcggcatcgc
    48361 gccgcgcttg ttgaaaacga aaaattgcgg ctattttacc catcaacggg ggggaggcaa
    48421 tcagtgttta cacaggagct gctccagttc gtgcaacgaa gaaacggtcc aggtgggcgc
    48481 gatgccttct ggttgctcgc gattgtgcgc attcagccag caggtcgcaa gcccggcgtt
    48541 gatgccaccg agaatatcgg actcagcagt gtcgccaacc atcagcacgc gtgaacggtc
    48601 aggattgccc gcctgttcca gcgcataatc gaaaattttc ttattcggtt tggcaacgcc
    48661 aacttcttcg gaaatcacca gcaaatcgaa ataatcacgc aggcccgtgc gttccagacg
    48721 cacttgctgc aaggcgctaa agccgttggt gatgatgcca attttagcgt tgccacgaat
    48781 ggcgttaagc agagaaaccg cgcccggcag cggcgtacag atttccgcca tcgcattaat
    48841 aaaggcttcg ttgagtttac ccggctcgac gttcagccgt tcggcccagc tctcaaaccg
    48901 cccgtgctga agctgtaatg aagtgatcgc gccgttttga taatccaccc acagtggctt
    48961 gttaacggcc tgatagtcct gaaaatcttc agcggtaaag gtgacgctgt aatcaagaaa
    49021 catccgctgc aggccggtga atgagtcaaa ggtaaacagc gtttcatcgg catcaaagaa
    49081 aatccagtcc cacttcatta ttccaccttg tcttacatac tgattggtaa cgccatgatg
    49141 atggcgtctt cgcgaccgtc cgtggtgggg tagtaattgc ggcgaatcgt cgcctcgtta
    49201 aagcctaaac tttcgtacag ggcaatggcg gcagcatttg aggcacggac ttccagccat
    49261 agcgtcgcca cgccgcgttt ttccagttcg tcgatcagat gttccagcag cacccttccc
    49321 agtccctgac gctgatagtc aggatcgacc gcaatattga acaatgtagc ttcatccagc
    49381 accacttgcg taatcgcaaa cgccgccatt ttgccgtttt gctttaactg aaagttgaga
    49441 tagcgctcgc cctgattact ggcaaaggtt ttttcactcc acggaaaggc gtgggcgcgt
    49501 tgttcaatgt ggtaagccgc tggtaaatca gtcgtttcga gggaagaaat cgtgttcata
    49561 tgtgcaaatt tgttgccata acgcggcgcg tgccgttggg tttgcccgta attcggtgag
    49621 cgccggtgat gtcacctgag cgccttccag tgatagcggt tcgtcagtac ccaaccgcca
    49681 gctgttgcag cggctacctt gcggcagcat cgcgattttt tctggcgtca gttgcagcac
    49741 ttgatcgggg ctgacagtta acgcacgcag aacatcgctc accagaggat cggtcagggc
    49801 gggaagatcg tttgccacca tcaccagacg gacgtgtggc gggatcgcaa tggcaatctc
    49861 cccctgcaac gcgccagggc gacgcagcga ccactgggta atgcccagtt gctgtaactg
    49921 ccagtctcgt cgggatgtca tagcgaatcg ctcctgttgt caggggcgca aatatagcaa
    49981 attcgtcgat accgcgccaa catatggcta taatcgccgc cagtatcaat tgaggagcat
    50041 tccatgtctg catttacccc ggcaagtgaa gtcttgctgc gtcacagtga tgatttcgaa
    50101 caaagccgta ttctgtttgc cggagactta caggatgacc tgcccgcgcg tttagatacc
    50161 gcggccagcc gtgctcatac ccagcaattc caccactggc aggtgttaag ccgccagatg
    50221 ggggataacg cccgttttag cctggtcgcc acggcagatg acgtcgcaga ttgcgatacg
    50281 ctgatctact actggccgaa gaacaaaccg gaagcccagt tccagttgat gaatttactt
    50341 tctctgcttc cggtgggcac ggatattttt gtcgttggcg agaaccgctg cggcgtgcgc
    50401 agcgccgagc agatgctggc cgattatgcg ccgttgaata aggtagacag cgcccgtcgc
    50461 tgcggcctct attttggtcg tctggaaaag cagccggtat ttgatgccaa taaattctgg
    50521 ggcgaataca gcgtcgatgg cttgacggtc aaaacgctgc cgggcgtgtt tagccgcgac
    50581 ggtctggatg tcggcagtca gttgctgctc tcgacgctaa ctccacacac gaaaggtaaa
    50641 gtgctggatg tcggctgcgg cgcaggcgtt ctttccgttg cttttgcgcg ccattcaccg
    50701 aagatccgcc tcactctgtg cgatgtctct gcaccagcgg tagaagccag tcgcgcaaca
    50761 cttgctgcca acagtgttga aggtgaagtc tttgccagca acgtcttttc cgaggtgaaa
    50821 ggtcgctttg atatgataat ctccaacccg ccgttccacg atgggatgca aaccagcctg
    50881 gatgcggcgc aaacgctgat tcgcggcgcg gtgcgtcatc ttaacagcgg cggcgagttg
    50941 cgaattgtag cgaacgcctt cctgccttac ccggacgtgc tggatgagac atttggtttc
    51001 cacgaagtga ttgcgcaaac cgggcgcttc aaggtgtatc gcgccattat gacccgccag
    51061 gcgaagaaag gttaagtatc tcgccgcatg ccttgtcgga tgcggcgtac acgccttatc
    51121 tgaccgacaa acgaattatc cacaatccgc tgatggacat ttttccagca attacacctc
    51181 tgtcgataat taactattga cgaaaagctg aaaaccacta gaatgcgcct ccgtggtagc
    51241 aattcttttt aagaattgat ggtatgcgaa ggtggcggaa ttggtagacg cgctagcttc
    51301 aggtgttagt gtccttacgg acgtgggggt tcaagtcccc cccctcgcac caattatctt
    51361 tacttctttt cttgtctcgt cactgtttta tcacccctga ttcaaattca ttatcatcag
    51421 catgatgcca gcaataagcg ccaggcacga tggtagcaaa acgtaatgcc gtaactgttt
    51481 gtgatacagc atcaccggcg tcaataccaa tcccatcaga atgacaatcc gccattgatc
    51541 cggcgataaa tccgcataca tcagccctgc aatcagcaat cccggcagca acactcccca
    51601 gccactgccc agcgtacgtt gcaacatggt ttcatctctt tcattgataa tgataactaa
    51661 tatcatatga taatttttat catttgcaag ccagagaaat cccttgctat cgggtaaacc
    51721 tatcgatagg attagcaata attatcattt agattactat cacgattatg gcctatcgtt
    51781 ccgcaccgct ctatgaagat gttatctggc gaacgcatct ccagccgcag gatgctgggc
    51841 ttgcacaagc cgtgcgggca acgatcgccg aacatcgtga acatttgctg gagtttatcc
    51901 gcctggatga acccgcccca cttaacgcca tgacgctggc gcaatggtca tcacctaatg
    51961 cgctaagttc tctactcgcg gtctattccg atcatatcta ccgcaaccaa cctttgatga
    52021 tccgcgaaaa caaaccgctt atctcactgt gggcgcaatg gtatatcggc ctgatggtac
    52081 caccattaat gctggcgctg ctgactcagg aaaaagcatt agatgtgtcg ccggaacatg
    52141 tccacgttga gtttcacgaa accggacgcg ccgcctgttt ctgggtcgat gtgtgtgaag
    52201 ataaaaacgc aacactacat tcgccgcagc agcgaatgga aacgttaatc agccaggcgc
    52261 tggttccggt tgtgcaggca ctagaagcaa cgggagagat taacggcaaa cttatctgga
    52321 gcaataccgg ttatttgatc aactggtatc tgactgagat gaaacagctg ctcggcgagg
    52381 cgacggttga atcgctgcgc tatgccctct tttttgagaa aacgcttact accggtgaag
    52441 ataatccact ctggcgtacc gtggtgctac gcgacggcct gctggtgcgc cgcacttgct
    52501 gccagcgtta tcgcttaccg gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc
    52561 actgccgggg aagtaaaccc ggcatcatgc ggattaataa ggatgcaaca cattacttgt
    52621 ttcgcaatac cgcatacata acagaatatc tgcggcctcc aacaaccctg catcagaact
    52681 gacgcccagt ttcgacatca cattaaattt atgtgcacgg atcgttttaa tattgcgttt
    52741 aagctgctcg gcaatttgtg tcattgagta gccacgcgac ataaagcgca atatttctct
    52801 ctccgtcggg cttaacgtcc ggctttggtt aatgtaccac tgattgttca gtcgatcggt
    52861 cgcctgacgt acaccattta atgacaaaaa gagttcctga tgaaaaatct ccagcgttga
    52921 cgctttactt aatacaccgt ccagcggtga tggcgacaat gaaccaatca gccgagcttc
    52981 aatatcatca tccgcaataa ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt
    53041 caggcaggat aatccctctc tgcgcgcact tctcatggca gaaaaagaaa aaatgaccgc
    53101 cgcaaacgag atccgcgaca tggcatcctg aaatgactcc tgactggtat aggtatggag
    53161 cttataaggg ttacccgcaa actttctgaa taaactctca ataccgatac tactcatgac
    53221 gcatttttct atgagtgcga catttctctt cttaattcgg ctgtgttcca ttctgcggca
    53281 tctccataaa cagaaggcaa gttaagttct cttgcaccct gcgcgctatt tatccatgca
    53341 tacatttcag cgttgctgtg aattgccagt cgacgcatcg cactgttttt ttgggcgctg
    53401 atagttttat tacttttctt aagcaatgaa gctatctggt tgatgcccca tcctttacct
    53461 aatagccgca gtaccttgcg ctctgaaaga gtgagaatga cggagcgata gctaaaatca
    53521 tgagtttcag tcattgccgg agaggtcagc atggcgctga tacgctctga gtgcgtattg
    53581 ccggaacgta cggtcttgac cagattctca atgggttcaa catcagacaa taacgtcgcc
    53641 gtagggcaca tgagcaattc tactgcctgg gaataccagg agcgtgacac catgaaaacc
    53701 cagtgaattt cccgatattg tgagattaaa gagtaataat gctcacaaac ggaacgtgga
    53761 tcttcacttt caccggccaa atcagcaatg actaatccgg aacgacgtaa ttgtaataag
    53821 gtaaggtcct ccgcagaggc gctggatatt atttcatatt cagggaagtg agtcctcatg
    53881 acctctttta accctgcttg cataacaggt attttactga taacaattcc atttttgcag
    53941 catcctggca acatatttcc tccacatcca ctgtctaaaa atcatgatgg tttattcaag
    54001 gtattcattc cttaaatatt tc
//
LOCUS       DADSZD010000011        51364 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.011, whole genome shotgun sequence.
ACCESSION   DADSZD010000011 DADSZD010000000
VERSION     DADSZD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.011"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            210..881
                     /locus_tag="J1Z28_000580"
     CDS             210..881
                     /locus_tag="J1Z28_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBA7715443.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            891..2087
                     /locus_tag="J1Z28_000581"
     CDS             891..2087
                     /locus_tag="J1Z28_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBA7715444.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2065..2646
                     /locus_tag="J1Z28_000582"
     CDS             2065..2646
                     /locus_tag="J1Z28_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA7715445.1"
                     /translation="MKNFLKLNVKEKNIQIADQVIIDESAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVRIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2648..3421
                     /gene="yaiO"
                     /locus_tag="J1Z28_000583"
     CDS             2648..3421
                     /gene="yaiO"
                     /locus_tag="J1Z28_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBA7715446.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNNPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3610..3885
                     /gene="frmR"
                     /locus_tag="J1Z28_000584"
     CDS             3610..3885
                     /gene="frmR"
                     /locus_tag="J1Z28_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBA7715447.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3920..5029
                     /locus_tag="J1Z28_000585"
     CDS             3920..5029
                     /locus_tag="J1Z28_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBA7715448.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5122..5955
                     /gene="fghA"
                     /locus_tag="J1Z28_000586"
     CDS             5122..5955
                     /gene="fghA"
                     /locus_tag="J1Z28_000586"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBA7715449.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6182..6721)
                     /locus_tag="J1Z28_000587"
     CDS             complement(6182..6721)
                     /locus_tag="J1Z28_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBA7715450.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKITQTQLINGRLAIARLLVDNNNEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6823..8034)
                     /gene="mhpT"
                     /locus_tag="J1Z28_000588"
     CDS             complement(6823..8034)
                     /gene="mhpT"
                     /locus_tag="J1Z28_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBA7715451.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8210..9223)
                     /gene="dmpG"
                     /locus_tag="J1Z28_000589"
     CDS             complement(8210..9223)
                     /gene="dmpG"
                     /locus_tag="J1Z28_000589"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBA7715452.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALNAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9220..10170)
                     /locus_tag="J1Z28_000590"
     CDS             complement(9220..10170)
                     /locus_tag="J1Z28_000590"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBA7715453.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEENVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLSAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10167..10976)
                     /gene="mhpD"
                     /locus_tag="J1Z28_000591"
     CDS             complement(10167..10976)
                     /gene="mhpD"
                     /locus_tag="J1Z28_000591"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBA7715454.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNETIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10986..11852)
                     /locus_tag="J1Z28_000592"
     CDS             complement(10986..11852)
                     /locus_tag="J1Z28_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxononadienedioate/2-hydroxy-6-
                     oxononatrienedioate hydrolase"
                     /protein_id="HBA7715455.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVKAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARA"
     gene            complement(11870..12814)
                     /gene="mhpB"
                     /locus_tag="J1Z28_000593"
     CDS             complement(11870..12814)
                     /gene="mhpB"
                     /locus_tag="J1Z28_000593"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBA7715456.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12816..14480)
                     /locus_tag="J1Z28_000594"
     CDS             complement(12816..14480)
                     /locus_tag="J1Z28_000594"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBA7715457.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDNVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTVHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRVLGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14557..15504
                     /gene="mhpR"
                     /locus_tag="J1Z28_000595"
     CDS             14557..15504
                     /gene="mhpR"
                     /locus_tag="J1Z28_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HBA7715458.1"
                     /translation="MIFCCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLATRPGDDYQLAR
                     EPLKLEAILARTRKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIKEGVESQAILVAGRRSGMHLR"
     gene            15581..16663
                     /locus_tag="J1Z28_000596"
     CDS             15581..16663
                     /locus_tag="J1Z28_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7715459.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLPPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16786..19860
                     /locus_tag="J1Z28_000597"
     CDS             16786..19860
                     /locus_tag="J1Z28_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBA7715460.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWSFAWFPAPEAVPESWLERDLPDADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFQVTTRFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPELWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGEMRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQYFQFRLSGRTIEVTSEYLFRHSDNEFLHWMVALDGKPLASGEVPLDVGPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHAIPQ
                     LTTSGTDFCIELGNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGHGEMVINVDVAVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19912..21165
                     /gene="lacY"
                     /locus_tag="J1Z28_000598"
     CDS             19912..21165
                     /gene="lacY"
                     /locus_tag="J1Z28_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HBA7715461.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21231..21842
                     /gene="lacA"
                     /locus_tag="J1Z28_000599"
     CDS             21231..21842
                     /gene="lacA"
                     /locus_tag="J1Z28_000599"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HBA7715462.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEF
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVTKDIPPNVVAAGVPCRVIREINDRDKQYYFKDFKVESSV"
     gene            complement(21963..23246)
                     /locus_tag="J1Z28_000600"
     CDS             complement(21963..23246)
                     /locus_tag="J1Z28_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HBA7715463.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLHDGKISAIDTQSGVMPVTE
                     NSLDAEQGLVLPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVMASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(23236..24495)
                     /gene="codB"
                     /locus_tag="J1Z28_000601"
     CDS             complement(23236..24495)
                     /gene="codB"
                     /locus_tag="J1Z28_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBA7715464.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
     gene            complement(24820..26706)
                     /gene="prpE"
                     /locus_tag="J1Z28_000602"
     CDS             complement(24820..26706)
                     /gene="prpE"
                     /locus_tag="J1Z28_000602"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HBA7715465.1"
                     /translation="MSFSEFYQRSINEPEKFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVATRIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDREVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(26746..28197)
                     /gene="prpD"
                     /locus_tag="J1Z28_000603"
     CDS             complement(26746..28197)
                     /gene="prpD"
                     /locus_tag="J1Z28_000603"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HBA7715466.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSRVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAIASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(28231..29400)
                     /gene="prpC"
                     /locus_tag="J1Z28_000604"
     CDS             complement(28231..29400)
                     /gene="prpC"
                     /locus_tag="J1Z28_000604"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HBA7715467.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPGEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(29560..30450)
                     /gene="prpB"
                     /locus_tag="J1Z28_000605"
     CDS             complement(29560..30450)
                     /gene="prpB"
                     /locus_tag="J1Z28_000605"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HBA7715468.1"
                     /translation="MSLHSPGKAFRAALSKETPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNILRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARGQVK"
     gene            30689..32275
                     /gene="prpR"
                     /locus_tag="J1Z28_000606"
     CDS             30689..32275
                     /gene="prpR"
                     /locus_tag="J1Z28_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HBA7715469.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFSRHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGKLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMQQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETMLVHYNWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPIPGLLTAQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(32376..32651)
                     /locus_tag="J1Z28_000607"
     CDS             complement(32376..32651)
                     /locus_tag="J1Z28_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7715470.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVASQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            32837..33469
                     /locus_tag="J1Z28_000608"
                     /pseudo
     CDS             32837..33469
                     /locus_tag="J1Z28_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
     gene            complement(33486..33729)
                     /locus_tag="J1Z28_000609"
                     /pseudo
     CDS             complement(33486..33729)
                     /locus_tag="J1Z28_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34351..35400)
                     /gene="yahK"
                     /locus_tag="J1Z28_000610"
     CDS             complement(34351..35400)
                     /gene="yahK"
                     /locus_tag="J1Z28_000610"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBA7715471.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPHDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDYMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSESKREAAKALG
                     ADEVVNSRNADEMVAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTITLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(35487..36443)
                     /locus_tag="J1Z28_000611"
     CDS             complement(35487..36443)
                     /locus_tag="J1Z28_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715472.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKSIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(36440..37411)
                     /locus_tag="J1Z28_000612"
     CDS             complement(36440..37411)
                     /locus_tag="J1Z28_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715473.1"
                     /translation="MAELKKRHEFWLALLIMVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGGIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(37404..38888)
                     /locus_tag="J1Z28_000613"
     CDS             complement(37404..38888)
                     /locus_tag="J1Z28_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7715474.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMISINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYQNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSEAKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(38937..39920)
                     /locus_tag="J1Z28_000614"
     CDS             complement(38937..39920)
                     /locus_tag="J1Z28_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7715475.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(40174..40584)
                     /locus_tag="J1Z28_000615"
     CDS             complement(40174..40584)
                     /locus_tag="J1Z28_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA7715476.1"
                     /translation="MTIKEKISQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTAELARKEGEGDLSL
                     EYWRNEHRRFFQAEGCYSPEMDVIFEEYALIDVV"
     gene            complement(40882..42264)
                     /locus_tag="J1Z28_000616"
     CDS             complement(40882..42264)
                     /locus_tag="J1Z28_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBA7715477.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGPSVPLAHAGGAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPAGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDKLAHRMVAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDTSCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(42274..43224)
                     /locus_tag="J1Z28_000617"
     CDS             complement(42274..43224)
                     /locus_tag="J1Z28_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBA7715478.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLMFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(43461..44879)
                     /locus_tag="J1Z28_000618"
     CDS             complement(43461..44879)
                     /locus_tag="J1Z28_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA7715479.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTAKTILHAG
                     PPVTWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSSCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAIFGWCERYGV"
     gene            complement(44879..46426)
                     /gene="fdrA"
                     /locus_tag="J1Z28_000619"
     CDS             complement(44879..46426)
                     /gene="fdrA"
                     /locus_tag="J1Z28_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA7715480.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGVDNEQLLAEIEELFNTKAQSGSHEARYG
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVACFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETLFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     REAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(46416..47279)
                     /locus_tag="J1Z28_000620"
     CDS             complement(46416..47279)
                     /locus_tag="J1Z28_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA7715481.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFNGLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNDKQNI
                     ASAVASMMGLGIGLTPSADDYLTGLVLILFISGHPAEKYKEEFYLGLQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(47319..47924)
                     /locus_tag="J1Z28_000621"
     CDS             complement(47319..47924)
                     /locus_tag="J1Z28_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBA7715482.1"
                     /translation="MTIKNLPADYLLAAQQGDIDKVKICLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGANINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLANTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            48182..48679
                     /locus_tag="J1Z28_000622"
     CDS             48182..48679
                     /locus_tag="J1Z28_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBA7715483.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIKSLAVNYSGMVWAFFAALAAGWLAPVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            48771..49703
                     /locus_tag="J1Z28_000623"
     CDS             48771..49703
                     /locus_tag="J1Z28_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7715484.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMAQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(49745..50833)
                     /locus_tag="J1Z28_000624"
     CDS             complement(49745..50833)
                     /locus_tag="J1Z28_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA7715485.1"
                     /translation="MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTE
                     ISQYRNRSAKTISHQKKQLFEKLGIQSDITFWRDIFFQYNPEIISATGNNSHKYINDN
                     HYHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGMIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
BASE COUNT        12278 a        13895 c        12867 g        12324 t
ORIGIN      
        1 aaataaccat acaaaccaaa cacacaaaca cttaacatat gaactacata aaagattaca
       61 cactcttatc cccgtacctg acagcccctt gtttttcagc tatcagctat ttttaacact
      121 taaaaattag catctgtctg cgtcagaggt tacgtctctg ggggcgtcgt tgtgcttgta
      181 gtataataaa taaaatggag tgtcataaag tggataaggt tttagattca gcgatccttt
      241 cttcggcaaa taaaagaaag ggtatccttg ccattggcgc gcatcctgac gatatagaat
      301 taggctgtgg cgcatcgcta gctcgccttg cgcaaaaagg aatttatatt gctaccgtgg
      361 tgatgactac cggtaattct ggcgtagatg gaataatcga tcgccacgaa gaatcacgca
      421 acgctctaaa gatattaggg tgccaccaaa ctattcatct taattttgct gacacccgcg
      481 ctcatttaca gctcaatgat atgatttctg ccctggaaaa catcataaaa aatcaaattc
      541 cctctgatgt tgaaattata cgtgtatata ccatgcatga tgctgaccgc catcaggatc
      601 atcttgcagt ctatcaagcc tcaatggttg cctgtcgcgc tattccgcaa attctcggct
      661 atgaaacacc aagtacctgg ctttcattta tgcctcaggt ttttgaatcc gtgaaagaag
      721 aatatttttc tcttaaactg actgcgttaa aaaaacataa aagtcagagc cagcgagatt
      781 atatgcgccc ggagcgtctg cgtgcagttg ctcaatttcg cgggcaacag gtcaatagcg
      841 atctgggtga aggttttgtt attcataaaa tgattctttg agcaaattgc atgaaaacct
      901 ggatctttat atgtatggcc gtagcaatat tgctatggtt tctgagtacg ttaaggcgta
      961 agcccagcca aaagaaaggc tgtattgacg ccattatacc tgcttataac gaaggcccat
     1021 gtctggcgca gtcactggat aatctgctgc gtaaccctta tttttgccgg gtaatttgcg
     1081 ttaacgacgg ctccacggac aataccgaag cggtcatggc ggaagtcaaa cgcaaatggg
     1141 gcgaccgctt tgttgccgtc acgcaaaaaa ataccggtaa aggtggtgcg ctgatgaatg
     1201 gcctcaatta cgccacctgc gatcaggttt ttttaagtga tgccgacacc tatgttccgc
     1261 ccgatcaaga cggaatgggc tatatgctgg cggaaattga gcgtggcgct gatgccgtag
     1321 gcggcattcc ctctactgcg ttgaaaggcg cgggtctgtt accacacatc cgcgcgaccg
     1381 taaagttgcc gatgattgtt atgaagcgca cgctacagca gctcctgggc ggcgcaccgt
     1441 ttatcatcag cggtgcctgc gggatgttcc gtactgatgt attgcgtaag ttcggtttct
     1501 cggatcgtac taaagtcgaa gaccttgatc tcacctggac gttggtggca aacggctacc
     1561 gtattcggca ggcgaatcgc tgcatcgtat acccacagga atgtaacagt ccgcgtgagg
     1621 agtggcgccg ctggcggcgt tggattgtgg gctacgcggt ctgtatgcgc ctgcataaaa
     1681 gacttttatt tagccgcttc ggtatcttca gtatatttcc tatgctgctg gttgtgcttt
     1741 atggcgttgg gatttatctc actacctggt ttaatgaatt catcaccacc gggccacatg
     1801 gtgtggtgtt ggcaatgttc ccgcttatct gggtcggcgt agtttgtgtt attggtgcat
     1861 ttagcgcctg gtttcatcgt tgctggttgt tggtgccttt agcaccgctt tccgttgtgt
     1921 atgtattatt agcttatgcc atctggatta tttatggact tattgccttt tttactggac
     1981 gcgaacctca gcgcgacaaa cccacccgct attccgcact ggtggaagcg tcaaccgctt
     2041 attcccaccc ttctgtcaca ggaactgaaa aactttctga agcttaatgt taaagagaaa
     2101 aatattcaga ttgcagacca ggtgattatt gatgaaagtg ccggtgaggt tgttatcggc
     2161 gcgaataccc gtatttgcca cggtgccgtt attcagggtc cggtagtgat tggcgcaaac
     2221 tgcctgatag gtaattatgc gtttattcgt cctggcacaa taatcagcaa tggcgtaaga
     2281 attggttttg ccaccgaaat taaaaatgcg gttattgaag cggaagccac gattggtccg
     2341 caatgcttta ttgccgactc ggtagttgca aaccaggcat atttgggcgc acaagtacga
     2401 accagtaatc atcgtctgga tgaacagccc gtgtctgttc gaactccaga tggaattatc
     2461 gctaccggat gcgataaatt aggttgttat atcgggcagc gttcacgcct tggcgtacaa
     2521 gttattattt tgcctgggcg aattatttcc ccgaacacgc aacttggccc gcgcgtgatt
     2581 gtagaacgta atttacctac cggaacttat tcactccgac aagaacttat ccgtacagga
     2641 gattaaaatg ataaaacgga cgttattagc ggcggcaatt tttagcgcat tacccgttta
     2701 tgccgggtta acttccatta ccgcgggcta cgattttacc gattattctg gcgatcatgg
     2761 caaccgtaat ttagcgtatg ctgaactggt ggcgaaagtt gaaaacgcaa cgctgctttt
     2821 taatctttcc caggggcgtc gtgattatga aactgaacat ttcaatgcca ctcgcggcca
     2881 gggtgcagtc tggtacaaat ggaataactg gctgacaacc cgaacgggta ttgcctttgc
     2941 ggataatacg ccggtctttg cccgccagga ttttcgtcag gatattaacc tggccctgtt
     3001 gccaaaaacg cttttcacga ccggttatcg ctacactaaa tattacgatg atgtcgaagt
     3061 cgatgcctgg caaggtggca tatcactcta tactggcccg gtgatcacca gctatcgcta
     3121 tacccattat gactccagcg atgcaggtgg tagttacagc aatatgattt ccgtgcgtct
     3181 gaataacccg cgcggcactg gttatacgca actatggcta agccgcggaa caggcgctta
     3241 cacctatgac tggacgccag aaacacgcta cggcagcatg aagagcgtca gtttacaacg
     3301 tattcaaccg ctgactgagc aacttaatct cgggctgacg gcaggtaaag tgtggtacga
     3361 caccccaacc gatgattata acggtctgca acttgcagcc catctgacct ggaaattctg
     3421 attccttctg ccgcccgcta tccggggcgg ccttccctgc cgattagccc ctaccccctt
     3481 tcctctttgt tttccgacta cattcaccgg ataaatttta ttctccagtg ttatatacta
     3541 taggggggta tgcattgaca tatagcatac ccccctatag tatattgcgt gcagatgatg
     3601 aggtgcgaaa tgcccagtac tccggaagag aagaaaaagg tccttactcg agttcgtcgt
     3661 attcgggggc agattgatgc tctggaacgg tcgctggagg gtgatgccga atgccgtgcc
     3721 atactccaac agatcgctgc cgttcggggc gcggctaacg ggctgatggc agaagtgctt
     3781 gaaagccata tccgggaaac gtttgaccga aatgactgct acagccgcga agtcagccaa
     3841 tccgttgacg acactattga actggttcgt gcctatctta aatagctgaa tctattacca
     3901 tattgaggaa gagcgagaga tgaaatcacg tgctgccgtt gcatttgctc ccggtaaacc
     3961 gctggaaatc gttgaaattg acgttgcacc accgaaaaaa ggtgaagtgc tgattaaagt
     4021 cacccatacc ggtgtttgcc ataccgacgc atttaccctc tccggcgatg acccggaagg
     4081 tgtcttcccg gtggttctcg gtcacgaagg ggccggggtt gtagttgaag tcggtgaagg
     4141 ggtaaccagc gtcaaacctg gcgaccatgt gatcccgctt tacaccgcag agtgcggcga
     4201 gtgtgagttc tgtcgttctg gcaaaactaa cctgtgtgtc gcggttcgcg aaacgcaggg
     4261 caaaggcttg atgccagacg gcaccacccg tttttcttac aacgggcagc cgctttatca
     4321 ctacatggga tgctcaacat tcagtgaata caccgtggta gcggaagtgt ctctggccaa
     4381 aattaatcca gaagcaaacc atgaacacgt ctgcctgctg ggctgtggcg tgaccaccgg
     4441 tattggcgcg gtacacaaca cagctaaagt ccagccaggt gattctgttg ccgtgtttgg
     4501 tcttggcgcg attggtctgg cagtggttca gggcgcgcgt caggcgaaag cgggacggat
     4561 tatcgctatc gataccaacc cgaagaaatt cgaactggcg cgtcgcttcg gtgccaccga
     4621 ctgcattaac ccgaatgact acgacaaacc aattaaagat gttctgctgg atatcaacaa
     4681 atggggtatc gaccatacct ttgaatgcat cggtaatgtc aacgtgatgc gtgcagcgct
     4741 ggaaagcgcg caccgcggct ggggtcagtc ggtgatcatc ggggtagcag gctccggtca
     4801 ggaaatctcc acccgtccat tccagttggt caccggtcgc gtatggaaag gttccgcgtt
     4861 tggcggcgtg aaaggtcgtt cccagttacc gggtatggtt gaagatgcga tgaaaggtga
     4921 tatcgatctg gaaccgtttg tcacgcatac catgagcctg gatgaaatta atgacgcctt
     4981 cgacctgatg catgaaggta aatccattcg aaccgtaatt cgttactgat ttcccgctgg
     5041 tttaccccgt ccagttcaga cggggttctt aatactctcc ccgggcagcc gtccggggga
     5101 ttaaccgtga gataatgact gatggaactc attgaaaaac atgccagctt tggcggctgg
     5161 caaaatgtgt atcggcatta ttccccatca ctgaaatgtg aaatgaatgt cggcgtctat
     5221 ctcccaccaa aagccgagaa tgaaaaattg ccggtgttgt actggctttc aggcctgacc
     5281 tgcaacgagc agaatttcat tactaaatcg gggatgcagc gttacgcggc tgagcacaac
     5341 attattgttg ttgcgccgga caccagtccg cgaggcagtc atgtcgcaga tgctgaccgt
     5401 tacgatctcg ggcaaggtgc cgggttttac ctgaacgcga cgcaagcgcc gtggaatgaa
     5461 cattacaaaa tgtatgacta tatccgcaac gagctgccga atttagtgat gcatcatttt
     5521 ccagcaacgg ccagaaagtc tatctcaggt cattctatgg gcgggctggg cgcgctggtg
     5581 ctggcgttac gtaacccaga tgaatatgcc agcgtctcgg cgttttcgcc cattgtctcc
     5641 ccatcgcaag taccgtgggg acagcaagcc tttgccgcat atcttggtga aaataaagat
     5701 gcctggttgg attacgaccc ggtgagtctt atttcacaag gtcaacgcgt tgcggaaatc
     5761 atggttgatc agggattgag tgatgatttt tacgcagaac agctgcggac tccaaatctt
     5821 gaaaagatct gccaggagat gaatatcaag acgttaatcc gttatcacga gggttatgat
     5881 cacagctatt attttgtctc cagttttatt ggcgagcata ttgcgtacca cgccaataaa
     5941 ctgaatatgc gttgataata gtgcacgact gccggatgcg gcgtaaacgc cttatccggc
     6001 ccacatttcg ctcgtaaacc gtaggcctga taagacgcga aaagcgtcgc atcaggcatc
     6061 tgcgcacggc tgccggatgc ggcgtgaacg ccttatccgg cctacacttc gcccataaac
     6121 cgtaggcctg ataagactcg caaagcgtcg catcaggcat ctgcacacga tttatctatg
     6181 tttaccacat caaatcatca ggcactttga agtcggcata cggatcgtct tcatcctgct
     6241 cttcggcgct gagcgcgctg tgcaacacaa tactgctggc atcacgctga gcaattttat
     6301 cggcaacgct ggcgggaata atggcgtatt caccttcgtt attgttatcg accaacaagc
     6361 gggcaatcgc cagacgacca ttaatcaact gcgtttgcgt gattttatcg acaaagattt
     6421 tcttaatcag attgccgtca gtgaagttaa aaccaatatc gccattggca atggtaattc
     6481 ggttcatttc aatgagctgc ttcacctggg ctttatattc tttcgccaac gccgcttgtt
     6541 tttgttgctc gctaagctgt ttatcacgct caagctgtgc ctttttattt tcttctaccg
     6601 ccgcccgagc ttcacgcgcc tgaacacgcg attttttcgc cgttctctcc accttcgccg
     6661 cttttttgct ggtgactagt cccgctttga gcaactgctc ttgtaaggta agttttgcca
     6721 tctttgtttc caaacccgct gaataatttt tagcgagtat acctgtaaac gccgtaggtg
     6781 tattgggggc aagtcccttt cgcccctact taataaggcg cgtcaggcat cggcgcacgg
     6841 ctgcattcgt gatctccggc tcatcaaaat aaacactgcc aggccggcaa caagaatacc
     6901 cggcgcagaa gcggccatta cgccaaccgt gccagtgcct aatgccagca ttttcccggc
     6961 cagtaacgga ccgctcatag cccccagacg ccctaccgcc acggctgttc ccacacctgt
     7021 tgcgcggatc tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc tttgcccacc
     7081 tgtcgcaaac aaccccgcga cgaatcccgc cagcaacata ccgttaaatg acgacaccgt
     7141 tcccagcgcc agcagcgaag ctaacatgcc gctataaatc agtagcgaca tggttactgg
     7201 acgcagctta tccatcaatg cgcccaacat taacgtcccg cttgccgcac ccatttgtag
     7261 ggcaaacatc acccctgccg cctgcgatgg ctggaatcct tgctccacca aaagcagcgg
     7321 tagccagttg atcaacatgt agaccaccag cagagtgaag aaataacaca accacagcag
     7381 cagcgtcgcg gtagccgttt ctggcgcaaa taacgcacgc agtggtggcg cagcctgttt
     7441 ttcgccagca aaaaccgccg actccggcag ccagcgcatt aatagcggca ccagaatcaa
     7501 cggcaccaca ccacctaccc aaaacaccgt ttgccatgct aagtttgccc ccgcgaaacc
     7561 cagcgtcgcc gccagcgccg cgccaatggg aacaccgcaa tacatcaggc tcactgccgt
     7621 cccacgaaaa cgtggccccg cggcttcaga cgtcagggcg ataagattcg gcaacgccgc
     7681 ccccagcccg acaccggtca tcagtcgcgc aaagaccagt gaggggaaat cccaggcaat
     7741 cgccgttgcc agtgagaaca aaccaaacag cgcaactgag ctaattaaaa tacgcttgcg
     7801 accataacgg tccgccagca ttccgccaac caacgcgccg ggtagcaaac cgagtattcc
     7861 ggcgctgaat atccagccca tttgcatttt atcgagtgcg aaagcctggg cgatgccacc
     7921 cgccgcaatg ccagccgcct gaagatccag tccttccatc agagcgacca aaaaacaaag
     7981 cccgatggtc agcatcaggc gggatgaaga tgatgaaggg gtacgagtcg acataagatg
     8041 aacctcattg cgagtgcagg tgaatcgcga ctgatacatt gccagccgcg taatcagaac
     8101 tatttaatga ctgcctacgg gcgaaatgta ggccggataa ggcgttcacg ccgcatccgg
     8161 cagtcgtgct gggatgggca tacaaaatgc acgttctcgg cagtcaatat tatttgttgt
     8221 tgcgcagatc cagcgccacg tcgacgatca tatcctcctg gccgccaacc atccggcgtt
     8281 tgcccagctc aacgagaata tccaccgcgc ttaagccata acgcgccgcc gccgtttcac
     8341 agtgacgcag gaagctggag taaacaccag cgtatcccag cgccagcgtt tcgcgatcga
     8401 ctcgtaccgg tcgatcctgc aacggacgca ccaggtcgtc agcggcatcc attaacgcat
     8461 agagatcggt cccatgctgc caacccagtt tatccgcggc ggcaataaat acttccagcg
     8521 gcgcgttacc tgcgcccgcg cccattcccg cgaggctggc gtcgattcgg tcgcagccct
     8581 cttccaccgc cgcgatagag ttcgccacgc caagactcag gttatggtga gcgtgcatgc
     8641 cggtttgcgt ttctggtttc agcactgcgt tcagggcgcg gaaacggtca cggatatcgc
     8701 tcatgttcat cgcaccgcca gaatccacca cataaataca ggtcgcaccg tagccttcca
     8761 tcagctttgc ctgctttgcg agattctccg gcgtggtcat atggctcatc atcagaaaac
     8821 caacggtgtc cattccgagc tcgcgggcat actgaatatg ctgggcggaa acatcagctt
     8881 cggtacagtg cgttgccaca cgaaccaccc gcgcgccagc ctgccaggca tttttcagat
     8941 cgtgaatagt gccgattcct ggcagtaaca acgtcgcgat tttggcgtgc ttcaccacat
     9001 ccgccgccgc ttcaatccat tcaaggtcgc tatgtgcgcc gaaaccatag ttaaagctgg
     9061 aaccctgcaa accgtcgccg tgggccactt caatcgaatc cacgcgggca tcatccagtg
     9121 ctttggcaat ctggcggaca ttttccagcg aatactgatg acgaatggcg tgcataccgt
     9181 cacgcaacgt gacgtccgaa atataaagtt ttttaccgtt catgccgctt ctcctgcctt
     9241 gcgcgccagt gactgggcca ttttttccgc tgtcgccagc gcactggaag tcataatgtc
     9301 gaggttgccc gcataggcag acagataatg cgctgcgcct tcgacttcca gccagaccgc
     9361 cgttttcagc ccggagaact gccccacgcc cggcaaatta accggtttat cctgcgggat
     9421 aacttcaaac tgcacgcgct gtttcaggcg atatcccggt acgtaagcct gcaccgcctc
     9481 agccatttca ttgattgagg cttcgatatc atcttgtgaa gcttcgtcgc tcaatacata
     9541 caccgtgtca cgcatcatca acggtggctc tgctgggtta agcacgataa tcgccttccc
     9601 tttcgccgcg ccgcccacca cttcaatggc tcgggaagtg gtttccgtaa attcatcgat
     9661 attggcacgc gtgccaggtc cggcagattt actggcgata gaagcgataa tttcggcgta
     9721 atgaacacgc gccacgcgtg aaactgccgc caccattgga atggtggcct ggccgccgca
     9781 ggtgaccatg ttgacgttca gttgatcgac gttctcctcg aggttaacca ccggcacgca
     9841 gtaagggccg atggcagcag gcgtcaggtc aattaagcga atatccggtt tcgcttcgcg
     9901 caaagcggca tcgtttttca catgcgcgcc cgcgctggtc gcatcaaata caatgtcgat
     9961 atcagcaaat tcaggcatgt tcatcagccc gatcacccct tcatgggtgg tggcgacgcc
    10021 catacgtctg gcacgcgcca gaccgtcgga ctgaggatca atgccgacca tcaccgccat
    10081 ctccagatgc tgaccgtgac gcaaaatttt aatcatcaga tcggtgccaa tgttgccaga
    10141 accgataatg gcgactttac gcttactcat gacagacttc cttttggggc tgcgcttgaa
    10201 aatgtcgcag caactgaacc tatgccttca atatgggctt caaaacgatc gcccgcattc
    10261 accgccacca tcggacctaa tgccccggta agaatgatat ctccggcgcg cagcggttca
    10321 cccaggctgg ccattttgcg tgccagccag acggcggcat taagcggatg tcccaggcat
    10381 tcgctgccgc gcccgctgga aacctcttcg ttattacgcg tcatcttcat ggcgcagttt
    10441 ttcaggtcta accccgccgg acgttgcgcc ggaccgccga tgacatacac cccacaggag
    10501 gcgttatctg ccacggtatc gacaaactga atcgaccagt cgcgaatgcg gctccccacc
    10561 acctccagcg ccggaagtac ccattcaatg gcgttataca attcgtcgaa ggtgatatca
    10621 gttgcgggta aatcgcggtt caacaccagt gcgatctccg cttcaatgcg gggttgcaga
    10681 acacgggaaa aaggaatggt ttcgttatcg ccataacaca tgtcggcaaa taatgtcccg
    10741 aaatccggtt gatcaacgcc cagttgttgc tgcactttcg gatgtgtcag gcccacttta
    10801 cgccccacca cgcgacgccc ctgcgcaacg tcatattgca catttatgtg ctgaatggcg
    10861 taggccgctt cagcgttatc gataccaatc agatcgcgca gcggcgcaat cgcttcgccc
    10921 tgctctgcgg cgcggcgtaa atccgccgcc agttgctcaa gagtatgctt cgtcatgacc
    10981 gttccttaag cacgtgcgag gaaattcagc accagttgat tgaaagcgtc ggcatgttcc
    11041 cactgcgccc agtgaccaca gtcgcggaag atatgcagtt cagaaccggc aatgccggac
    11101 agcagacgca gacccgcatc catcggcaca aagcggtcgt tgcgccccca gacaatcagg
    11161 gtttgcgctt tgatttccgc cagacgtggg ccaaaatccg ggaactgttt cggattagct
    11221 tccaggctct taacgaaatt ttccaggtga tcgcggcgcg acagcatatt attcaggcgc
    11281 gcttcaaaca gggcgtcggt caaatcgctg gtatcaaaaa cgaagatatc catcatcagc
    11341 ttcaggtttt cgatagtcgg ctgacgataa agctgattca gtcgcttaat accttcggtt
    11401 ggcatcggcg taaacaaact catgccgccc gtaccaccgc ccatcagcac cagtttgccg
    11461 acgcgctccg gccagttaag ggtgaacgcc acagaactat ggccgcccat cgagttgcct
    11521 agcaggtgga ttttggcgat atccagttga tccaccacgc ttttcaggat tcgcgcatta
    11581 agatccgatc gtgaaccact attaacgatc gaatcgctct taccccaacc cggacaatcc
    11641 agcaggatca cccgatagcc cgcctttacc agcggatcga tattgcggct gaagttcgcc
    11701 cagccagtag cacccgggcc ggaaccatgc agcaggacaa cagtttcgtc gccttgtccg
    11761 cagtcattaa aatggatgcg cagcgtttta cccgcttctt ctacattcag aaaacggctg
    11821 gtggcggctt cggtttgtgg ctgataactc atcatcttct cctgcatatt cagttctctg
    11881 ttctggcgct taacgagcca aatccggcaa tccactccgg gattgggcgg taataacgcc
    11941 cttcgctacg ccagttgcca aacgcagaaa tagcggcaaa agcggcgacc caggttttga
    12001 tttcatgtgt cgactttccg gcaatggcgg aaagctcttc gttactgacg gcatccagtt
    12061 cctgtatgcg tccctgctcc agcaaagtca tgaactggtt atcccaaatc gggttgagcg
    12121 gatgcagcgt tctctgatcc tcaacaaact tctcagcggc gctaatcacc cgctgctgac
    12181 gcaattcacg ctcagtggcg ggtaaatctt tcccgctccc caacagacgg tcgcgcatat
    12241 gggcatcggc tttcgccagt tctggcaccg gcggctgatg ggaaagccca ccggacccaa
    12301 ggaacagcac gcgtttattg agagtgctgg tgaaacgtcc aatggcttca cccaacatgc
    12361 gggtacgctg gaaaccgggc agcggcgtgg cgacaccgtt gatgaacaca ggcagaactg
    12421 gcaccttatc cagcccaccg agcaggaact ccagcggctg ggcgaacccg tggtccacct
    12481 gcatacagta agaaacggca agatcgattc cgctcttcat gacggcatgc gcacaggcct
    12541 ccgccagctc cacaggcacg ggcagctcac ctgccgcact gccgaaatca ccaattgccg
    12601 tcgctccaac gcctaaacag aacggtggca tcacgtcata gaaaaagccg ttgtagtgat
    12661 ctggcgcaaa cagcaccacc agttcagggg agaaagccgc aatacgctcg cgggcgctgg
    12721 caatcacgcc attgacctca tcgagtacct cttgtgccgg gtcgacatac cccaccagcg
    12781 gcgagtggga aagacagtga agataagcgt gcatatcagg ctaccttttc gacagaaacg
    12841 tcggcatcag ggcgggtcag cgtcatcacc gacgccagtt tattcagggt cttgcccagg
    12901 gtttgcggaa tggcggtggc ggcaacaaag cgatccgggc gcatcaccac cagcgaagca
    12961 ttgtgttgcg cgaaccagct acgcaggcga ccttgcgtat cccccacgcg tagtacgccg
    13021 tcgtggttat cctgtgcggt atgaatttgc acttccggca ccacctgaat gaagcgtgtg
    13081 cccaacacgc gccactgctg gatttgctcg tcgctcatcc cccacagtgg attgcatccc
    13141 cagccaatta ccgcgaagtt cgcgccgatc gcgttatcga gcagcgtcac gtcgccgttt
    13201 tccagcgtga ctttcggctg aataaacatc ttgccgaccg gagagtgctt cgcttcgccc
    13261 tcacgcacca gcgcaccgcc gtaatattgc ggcatcggct tgaagcgcat ttcgaggaag
    13321 tagcgtttta ctggcggcag ataattcaac agccaggaaa cgccgtcacg taacgtaccc
    13381 tgccagcgtt tcggcggagc cagcacgttg cccgccgtca cggacaggtc aatcatcgct
    13441 ttggcgtgat cgcgacgttc ttgttgatag gtatcgagca gcgcatcgcg ggctttcccc
    13501 tggataacca acgccagttt ccatgcgagg ttaaaggcgt cgcgcatacc actgttatag
    13561 ccctgcccct gccacaccgg catgatgtgc gcggcatcgc ccgccagcag tacgcgatca
    13621 atacgaaaac gctgcgccag tcgcgcgttg tgggtgtaga cacgctggcg aatcaattca
    13681 acattgtccg gattaggcag cactttgctt aacagcttgc gcatattttg cggctcacgc
    13741 agctgttctt cggtttctcc cggcatcacc ataaattcaa agcgacgtac cgcatgaggc
    13801 agcgcggcag aaacatacgg gcgcaccgga tcgcaacaca aatagatatg cggcgtactt
    13861 aacggatcgt tggcgatatc taccacaatc cactgatttg gcgcagtttt accttcaaac
    13921 ggcacattca gggtgcgacg gacaaaactt gccccaccat cacaggccac cagccactgg
    13981 gctttgacta tttcccgctg cccttctgcc gttttcaggt gcacggtcac ttcgtcatct
    14041 tgctggctga aggcctccag ctcgcgggaa aacaggcagc gcacattcgg aaaacgcgac
    14101 aatccttcca gcatcaccgc atcgacctgc ggctgaatga aggcgttacg gcgcggccag
    14161 ccaaattcat cggtcattgg ctgaatatca gcaaaacagc ggccttttgg ggtaagaaaa
    14221 cgcatcgcat gccacggcgt agtgtgcggc agaacattat cgaccaggcc aaccgactgc
    14281 atggtgcgca gcgcctcgtc atcaatacca atcgcacgcg ggtagtcgat caacttatcg
    14341 agtttctcca ccaccagcac gtcaatgccc atctggccga gatagttcgc catcatcagc
    14401 ccgaccggac cggcaccagc gatcgccacc tgaacgctat ggttaacagc aggctggatg
    14461 tcagggtgtt gtattgccat ttcagtacct cacgactcgg acaaaatgcc gttgcgcgca
    14521 cagtacagcg caacttattt tgttaaaaac atgtaaatga ttttttgttg tgcgctcagt
    14581 ataggaaggg tgttttcggc tacaatcaaa acatgcccga atgtgcacca ggtgcacaac
    14641 gttgttttaa ctatagaaat gtcaattaat atgcagaaca atgagcagac ggaatacaaa
    14701 accgtgcgcg gcttaacccg cggtctaatg ttattaaata tgttaaataa acttgatggc
    14761 ggtgccagcg tcgggctgct ggcggaactc agcggcctgc atcgcaccac tgtgcggcga
    14821 ctgctggaga cgctgcagga agagggatat gtccggcgta gtccctccga tgatagtttc
    14881 cgtttgacca tcaaagtgcg gcaattaagc gaaggatttc gtgacgaaca gtggatttct
    14941 gcactggcgg ccccgctgct gggcgatctg ttgcgcgaag tggtttggcc aacagatgtg
    15001 tccaccctgg atgttgatgc aatggtggta cgcgaaacca cccaccgttt cagccgctta
    15061 tcctttcacc gggcaatggt cgggcgacgt ttaccgctcc tgaaaaccgc ctcgggcctg
    15121 acctggctgg ccttttgccc ggaacaagaa cgcaaggaat taatcgaaat gttagccacc
    15181 cgtcctggtg atgactatca actggcacgg gaaccgttaa aactggaagc cattctggcg
    15241 cggacgcgca aagaggggta cggacagaac taccgcggct gggatcaaga agaaaagatc
    15301 gcctccattg ccgtaccgct gcgcagtgaa caacgggtga ttggctgtct gaatctggtg
    15361 tatatggcga gcgcaatgac cattgaacag gcagcggaaa agcatcttcc ggcgctacaa
    15421 cgggtggcaa aacagatcaa agaaggggtt gaatcgcagg ctattctggt ggccggaagg
    15481 cgaagcggca tgcatttacg ttgacaccat cgaatggcgc aaaacctttc gcggtatggc
    15541 atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgctata
    15601 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
    15661 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
    15721 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
    15781 cacttccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
    15841 cgatcaattg ggtgccagcg tggtggtgtc gatggttgaa cgaagcggcg tcgaagcctg
    15901 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt ggactgatca ttaactatcc
    15961 gctggatgac caggatgcca ttgctgtgga agcagcctgc gctaatgttc cggcgttatt
    16021 tcttgatgtc tctgaccaga ctcccatcaa cagtattatt ttctcccatg aagacggtac
    16081 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcagg
    16141 tccattaagt tctgtctcgg cacgtctgcg cctggcgggc tggcataaat atctcactcg
    16201 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
    16261 acaaaccatg caaatgctaa atgagggcat cgttcccact gcgatgctgg ttgccaacga
    16321 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
    16381 tatctcggta gtgggatacg acgataccga agacagctcg tgttatatcc cgccgttaac
    16441 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
    16501 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
    16561 aaccaccctt ccgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    16621 gcagctggca cgacaagttt cccgactgga aagcgggcag tgagcgcaac acaattaatg
    16681 tgagtcagct cactcattag gcaccccagt ctttacactc tatgtgtccg gctcgtatgt
    16741 tgtgtgaaat tgtgagcgga taacaatttc acacaggata cagctatgat tatgattacg
    16801 gattctctgg ccgtcgtatt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    16861 aatcgccttg cggcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    16921 gatcgccctt cccaacagtt gcgaagcctg aatggcgaat ggagctttgc ctggtttccg
    16981 gcaccagaag cggtgccgga aagctggctg gagcgcgatc ttcctgacgc cgatactgtc
    17041 gtcgtcccct caaactggca gatgcacggt tacgatgcgc ctatctacac caacgtgacc
    17101 tatcccatta cggtcaatcc gccgtttgtt cccgcggaga atccgacagg ttgttactcg
    17161 ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
    17221 ggcgttaact cggcgtttca tctgtggtgc aacgggcgct gggtcggtta cggccaggac
    17281 agccgtttgc cgtctgaatt tgacctgagc gcatttttac gcgccggaga aaaccgcctc
    17341 gcggtgatgg tgctgcgctg gagtgacggc agttatctgg aagatcagga tatgtggcgg
    17401 atgagcggca ttttccgtga cgtctcgttg ctgcataaac cgaccacgca aatcagcgat
    17461 ttccaagtta ccactcgctt taatgatgat ttcagccgcg cggtactgga ggcagaagtt
    17521 cagatgtacg gcgagctgcg cgatgaactg cgggtgacgg tttctttgtg gcagggtgaa
    17581 acgcaggtcg ccagcggcac cgcgcctttc ggcggtgaaa ttatcgatga gcgtggcggt
    17641 tatgccgatc gcgtcacact acgcctgaac gttgaaaatc cggaactgtg gagcgccgaa
    17701 atcccgaatc tctatcgtgc ggtggttgaa ctgcacaccg ccgacggcac gctgattgaa
    17761 gcagaagcct gcgatgtcgg tttccgcgag gtgcggattg aaaatggtct gctgctgctg
    17821 aacggcaagc cgttgctgat tcgcggcgtt aaccgtcacg agcatcatcc tctgcatggt
    17881 caggtcatgg atgagcagac gatggtgcag gatatcctgc tgatgaagca gaacaacttt
    17941 aacgccgtgc gctgttcgca ttatccgaac catccgctgt ggtacacgct gtgcgaccgc
    18001 tacggcctgt atgtggtgga tgaagccaat attgaaaccc acggcatggt gccaatgaat
    18061 cgtctgaccg atgatccgcg ctggctaccc gcgatgagcg aacgcgtaac gcggatggtg
    18121 cagcgcgatc gtaatcaccc gagtgtgatc atctggtcgc tggggaatga atcaggccac
    18181 ggcgctaatc acgacgcgct gtatcgctgg atcaaatctg tcgatccttc ccgcccggta
    18241 cagtatgaag gcggcggagc cgacaccacg gccaccgata ttatttgccc gatgtacgcg
    18301 cgcgtggatg aagaccagcc cttcccggcg gtgccgaaat ggtccatcaa aaaatggctt
    18361 tcgctgcctg gagaaatgcg cccgctgatc ctttgcgaat atgcccacgc gatgggtaac
    18421 agtcttggcg gcttcgctaa atactggcag gcgtttcgtc agtacccccg tttacagggc
    18481 ggcttcgtct gggactgggt ggatcagtcg ctgattaaat atgatgaaaa cggcaacccg
    18541 tggtcggctt acggcggtga ttttggcgat acgccgaacg atcgccagtt ctgtatgaac
    18601 ggtctggtct ttgccgaccg cacgccgcat ccggcgctga cggaagcaaa acaccaacag
    18661 cagtatttcc agttccgttt atccgggcga accatcgaag tgaccagcga atacctgttc
    18721 cgtcatagcg ataacgagtt cctgcactgg atggtggcgc tggatggcaa gccgctggca
    18781 agcggtgaag tgcctctgga tgttggcccg caaggtaagc agttgattga actgcctgaa
    18841 ctgccgcagc cggagagcgc cggacaactc tggctaacgg tacgcgtagt gcaaccaaac
    18901 gcgaccgcat ggtcagaagc cggacacatc agcgcctggc agcaatggcg tctggcggaa
    18961 aacctcagcg tgacactccc ctccgcgtcc cacgccatcc ctcaactgac caccagcgga
    19021 acggattttt gcatcgagct gggtaataag cgttggcaat ttaaccgcca gtcaggcttt
    19081 ctttcacaga tgtggattgg cgatgaaaaa caactgctga ccccgctgcg cgatcagttc
    19141 acccgtgcgc cgctggataa cgacattggc gtaagtgaag cgacccgcat tgaccctaac
    19201 gcctgggtcg aacgctggaa ggcggcgggc cattaccagg ccgaagcggc gttgttgcag
    19261 tgcacggcag atacacttgc cgacgcggtg ctgattacaa ccgcccacgc gtggcagcat
    19321 caggggaaaa ccttatttat cagccggaaa acctaccgga ttgatgggca cggtgagatg
    19381 gtcatcaatg tggatgttgc ggtggcaagc gatacaccac atccggcgcg gattggcctg
    19441 acctgccagc tggcgcaggt cgcagagcgg gtaaactggc tcggcctggg gccgcaagaa
    19501 aactatcccg accgccttac tgcagcctgt tttgaccgct gggatctgcc attgtcagac
    19561 atgtataccc cgtacgtctt cccgagcgaa aacggtctgc gctgcgggac gcgcgaattg
    19621 aattatggcc cacaccagtg gcgcggcgac ttccagttca acatcagccg ctacagccaa
    19681 caacaactga tggaaaccag ccatcgccat ctgctgcacg cggaagaagg cacatggctg
    19741 aatatcgacg gtttccatat ggggattggt ggcgacgact cctggagccc gtcagtatcg
    19801 gcggaattcc agctgagcgc cggtcgctac cattaccagt tggtctggtg tcaaaaataa
    19861 taataaccgg gcaggctatg tctgcccgta tttcgcgtaa ggaaatccat tatgtactat
    19921 ttaaaaaaca caaacttttg gatgttcggt ttattctttt tcttttactt ttttatcatg
    19981 ggggcctact tcccgttttt cccgatttgg ctacatgaca tcaaccatat cagcaaaagt
    20041 gatacgggta ttatttttgc cgctatttct ctgttctcgc tattattcca accgctgttt
    20101 ggtctgcttt ctgacaaact cgggctgcgc aaatacctgc tgtggattat taccggcatg
    20161 ttagtgatgt ttgcgccgtt ctttattttt atcttcggac cactgttaca atacaacatt
    20221 ttagtaggat cgattgttgg tggtatttat cttggctttt gttttaacgc cggtgcgcca
    20281 gcagtagagg catttatcga gaaagtcagc cgtcgcagta atttcgaatt tggtcgcgcg
    20341 cggatgtttg gctgtgttgg ctgggcgctg tgtgcctcga ttgtcggcat catgttcacc
    20401 atcaataatc agtttgtttt ctggctgggt tctggctgtg cactcatcct cgccgtttta
    20461 ctctttttcg ccaaaacgga tgcgccctct tcagccacgg ttgccaatgc ggtaggtgcc
    20521 aaccattcgg catttagcct taagctggcg ctggaactgt tcagacagcc aaaactgtgg
    20581 tttttgtcac tgtatgttat tggcgtttcc tgcacctacg atgtttttga ccaacagttt
    20641 gctaatttct ttacttcgtt ctttgctacc ggtgaacagg gtacgcgggt atttggctac
    20701 gtcacgacaa tgggcgaatt acttaacgcc tcgattatgt tctttgcgcc actgatcatt
    20761 aatcgcatcg gtgggaaaaa tgccctgctg ctggctggca ctattatgtc tgtacgtatt
    20821 attggctcat cgttcgccac ctcagcgctg gaagtagtta ttctgaaaac gctgcatatg
    20881 tttgaagtac cgttcctgct ggtgggctgc tttaaatata ttaccagcca gtttgaagtg
    20941 cgtttttcag cgacgattta tctggtctgt ttctgcttct ttaagcaact ggcgatgatt
    21001 tttatgtctg tactggcggg caatatgtat gaaagcatcg gtttccaggg cgcttatctg
    21061 gtgctgggtc tggtggcgct gggcttcacc ttaatttccg tgttcacgct tagcggcccc
    21121 ggcccgcttt ctctactgcg tcgtcaggtg aatgaagtcg cttaagcaat caatgtcgga
    21181 tgcggcgcga gcgccttatc cgaccaacat atcagggcgg aatgatcgca atgaacatgt
    21241 cgatgacaga aagaataaaa gcaggcaagc tatttaccga tatgtgcgaa ggcttacctg
    21301 aaaaaagact tcgtggaaaa acgttaatgt atgagtttaa tcactcgcat ccatcagaat
    21361 ttgaaaaaag ggaaagtctg attaaagaaa tgtttgccac ggtaggggaa aacgcctggg
    21421 tagaaccccc cgtctatttc tcttacggtt ccaacatcca tataggccgc aatttttatg
    21481 caaatttcaa tttaaccatt gtcgacgact acacggtaac gatcggtgat aacgtactga
    21541 ttgcacccaa cgttactctt tccgttacgg gacacccggt acaccatgaa ttgagaaaaa
    21601 acggagagat gtactctttt ccgataacga ttggcaataa cgtctggatc ggaagtcatg
    21661 tggttattaa tccaggcgtc accatcgggg ataattctgt tattggcgcg ggtagtgtcg
    21721 tcacaaaaga cattccacca aacgtcgtgg ctgctggcgt tccttgtcgg gttattcgcg
    21781 aaataaacga ccgggataag caatattatt tcaaagattt taaagttgag tcttcagttt
    21841 aaataagcaa aattacctga tgcgctccgc ttatcaggcc tacaatttca gtgatctacg
    21901 taggccggat aaggtgttca cgccgcatcc ggcataaact acgctcgcgg tagcccagcc
    21961 gattaccgtt tgtaatcgat ggcttctggc tgctccagat atacggtggt ttgtgccggt
    22021 tgtgtgctgg ccatcacctt accgccacgt accgaataac gtaccggaac ctgacggcgc
    22081 aacgcatcaa acccattttc agccggcagg ataatcaggt tggcgctgtt tccggcggca
    22141 atgccgtaat cctgcaaatt caacgtcctg gcgctgtggt gggtgattaa attcaggccg
    22201 tcgttaatct gcccgtagcc catcagctgg caaacatgca gccccatatg cagcacttgc
    22261 agcatattcg ccgttcccag cggataccac ggatcgaaga catcatcgtg accaaagcag
    22321 acgttaatgc cggactccag catctcttta acgcgcgtga tgccgcgacg ttttggatac
    22381 gtatcgaaac gtccttgcag atgaatattg accaacgggt tggcgacaaa gttaataccg
    22441 gacattttca gcaaacgaaa caggcgcgaa gtatacgccc cgttatagga gtgcattgcc
    22501 gtagtatggc tggcggtgac tcgcgcgccc atgccttcac ggtgcgccag ggcagcaacg
    22561 gtttcgacaa agcgtgattg ctcatcatca atctcgtcac agtgaacatc gatgagacgg
    22621 tcgtattttt gcgccagggc gaaggtttta tgcagcgact ccacgccata ttcacgggta
    22681 aattcaaaat gcggaatcgc cccaacaacg tctgccccta agcgtaacgc ctcttccagc
    22741 aacgcttcac cgttgggata cgacaaaatc ccttcctgcg ggaaggcgac gatttgcaga
    22801 tcaatccacg gcgcaacttc ctgtttcact tccagcatcg ctttcaacgc agtcagcgtt
    22861 gcgtcggaga catcgacatg ggtacgcaca tgctgaatgc cgttggcaat ctgccatttc
    22921 agcgtttgcc aggcgcgttg tttcacatcg tcatgggtta ataacgcttt gcgctcggcc
    22981 cagcgttcaa tgccttcaaa cagcgtgccg gactgattcc agttcggttg tccggcggtt
    23041 tgcgtggtgt ccaggtgaat atgcggctcc acaaacggcg gtaaaactaa accttgttcg
    23101 gcatccaggc tgttttcagt tacgggcatc acaccagatt gcgtatcaat ggcgctgatt
    23161 tttccgtcgt gaagatgaat ctgccacagc ccctctttgc cgggtaaccg ggcgttaata
    23221 attgtttgta aagcgttatt cgacactgtt agcctccaca tgcgtcattg ctgctgtcgt
    23281 tttacgattc aaaatcgggt taaggatcag atagctcagc gcgccaccta ataccgcgtt
    23341 gaccggaaca attcccggta accagtggcc tgcggcaatc cccagcgcga ccgccagaat
    23401 cgccacccaa ttgacactca tcatacgcgt ggtcgcaaag tgctcatagc ggcgacggtt
    23461 catcagataa tcagcgatga tcacgccgcc caccggagga atagctgccg aaaggaaggt
    23521 caaccagccg acaaaattgt tatacagcca taatgcgcag accgtaccga taataccgtt
    23581 aatcaccgaa agggttttgc tcgacatacc ggtaatgttg gcgaaaccta aacccgacgc
    23641 atagagcgcg ttatcgttgg tggtccagat attcagcccc agcaccacaa tcgcaggcag
    23701 cagaaggccc tgagcaatca tcacatcaga gatatccgcc atccccagtg ccgccgcgcc
    23761 cgctgcaccg aaaataaaca tcaacgagtt gccgaggaaa aaggccacca tcgccaccag
    23821 caccgccagt ttggcattac gaccaaaccg gacaaagtca gcggtgagcg tgcccgcact
    23881 gataaatgac cccacaacca gcgccagcgc gacattgaaa tctaacggtt gtgcgggaac
    23941 gaccgctttt aatgcgtcca ggccgcccat gccgttaacg gccaaccaca cggaataacc
    24001 gccaagacag gcgatagccg gaaccgcaat caccgaaaga actgtcagcg ccgaaatgcc
    24061 aaagaagacg gtgacggtca tcagtaaacc ggagacggca atcagcaaat taatatccag
    24121 cccggttgcc ttgcccaccg gaatagcaaa catcgccacg ccaacgccaa accagccaac
    24181 ctgggtgccg cccagcagca gtgaaggcaa ccatgagcct ttaacaccaa acgagaagcg
    24241 agcaagaaga tgggtggtca ggccggtttt tgcgccaatg taaccaagaa atgaagtgta
    24301 aataccgagg agaagattac cgatgagaac tgcgaggaag aaatcatgat agctaagacc
    24361 ggttccgaga gtgccgccgg tccacatact ggcggaaaag aaggttaatc ccagcatgac
    24421 gaacgtcaat gccaataccc ctttccgcgc cgactgcggg actggcccct ggctaaagtt
    24481 gttatcttgc gacacgaaat tccccctttt tgctgtttga aacccaaaaa atccgccgca
    24541 ttctattcat ctgaagataa aaagcaatcg ttttcgtagg gggatatatt ttttatatga
    24601 atgagtgcaa aaaggcgggg gaaacttatc tgtgtaaaaa cagtgggtag gcatgataag
    24661 acgcgtcagc gtcgcatcag gcatctgcgc atggtgtcgg atgcggcgcg agcgccttat
    24721 ccgacctacg gttcaggcct gtaggcatga taagacgcgt cagcgtcgca tcaggcatct
    24781 gcgcacggtg tcggatgcgg cgcgagcgcc ttatccgacc tactcttcca tcgcctggcg
    24841 gatctgatcc aacgacgccg gatcatcaat ggtcgtcaga tccccaggat cgcgtccttc
    24901 gcaaatcgcc tggatcgtgc ggcgcagcat ttttccggat cgcgtttttg gcaattgcga
    24961 gacaaaccag acgtgcgccg ggcggccaaa gttgccaatc tggctgtcca ccagcgccat
    25021 aatcgccttc tcttgcgagt gcgccacctc acggtcttcc agactgtcgc tctctttcgg
    25081 aatgacaaac gccaccgcca cctgcccttt cagcgcatct ttcaccccaa ccaccgccac
    25141 ttcggcaacg cccggatgac tggagatact ctcttcaatc tcacgcgtac ccagccgatg
    25201 tccggcaacg ttaatcacat cgtcagtgcg cccgagaata aagtgataac cgtcagcatc
    25261 gcggatgccc cagtcaaaag tggcgtacac cggacgggaa aacagcgacc agtacgtctt
    25321 cacaaagcgg tcgtcgtcgc cccagatggt ttgaatacag cctggcggca atggcccctc
    25381 cactaccagc atccctttct cattgacgcc acacggttcg ccggtgactt cattgagcaa
    25441 ctgcacgtta tagccataca tcggcacgcc ggggcttccc agacgcgtcg gtctgtcatc
    25501 cagaccgcga gcaatcgcca taatcggcca gccggattcg gtctgccagt agttgtcgat
    25561 gaccggcaca tccagcgtat tgctcaccca actggcggtc ggctcgtcca gcggttctcc
    25621 agccagatag agcacttcca gcgacgaaag atcgtgtttg cgaatttcag cggtagggaa
    25681 ttttttcagc acgcgaatgg cggtcggcgc tgagaacatg cggctaacct gatatttctc
    25741 gacaattttc caccacacgc cgcagtccgg ccaggtcggc aatccttcgt aaacgatagt
    25801 cgccatcccc gccagcagcg gcgcgtaaac gatatacgaa tgccctacca cccagccgat
    25861 atccgaagca caaaagaaca cgccgcccgc tttgccgcca aaaatggtgt ccatcgaggt
    25921 cgccagcgcc accgcatatc cgccgacatc acgctgcaca cctttaggtt tgccggtcgt
    25981 gccggaggtg tagagaatgc aggaggtttc gttggattcc agccatgcca ccggcacccg
    26041 cgcgccgatg tgttgatggc gcaacgacgc gaaatcgaca tcccgcccgc taacgcgcgc
    26101 cattttcgcc agcccgcgat ccaccagtaa aacgtgacgc ggctgatgct gtgcctgact
    26161 tatcgcatcg tcgagcaatt ttttatacgg aatgatttta ccgccgcgcg ccccggcatc
    26221 agccgagaca atcagcaccg gtttagcgtc atcaattcgc gttgccacgc tgtgcgaagc
    26281 aaatccccca aacaccaccg agtgaatagc accaatgcgc gcgcaggcca gcagggtaat
    26341 atgcgcttcg gcaatcatcg gcatatacac cagcacccga tcgccacgct gcacgcccag
    26401 tgagcgcagc attgacgcca ccgcattcac ttcgtcatgt aactggcgga aggtaaaggt
    26461 acgctcttcc tctgtttccg aagagacggc aatcaatgcc agcgcctctg gctgtttctc
    26521 cagccagcga tcgatagcgt tgtgacacaa gttggttcgg ccttcacaaa accaacgggc
    26581 aaacggcggg ttgctgtggt cgagcgtttg cgtaaagggc gtctgccagt caatacgccg
    26641 ggcctgctcg gcccagaact tctccggttc gttaatcgaa cgctgataaa attcgctaaa
    26701 agacataatg ctctcctgtt gaacttacgc cttaccgccg tttacttaaa tgacgtacag
    26761 gtcgagatac tcattgaccg gcatctgttc caggcgagtt ctgtcgagag aaacctccag
    26821 aatgcgctgc tgctggcgag tcgggaactg gcgcgcgaga ttgattttga atttatcgac
    26881 cagcttcgga atgccatcct gacggcggcg agcatgacca attgggtact ccaccaccac
    26941 ttcttcaaat cgtgtgccgt cggtgaactc aagggttatg gcattggcga tggcgcgttt
    27001 ttccgggtcg tggtagtcag cggtaaacgc cggatcttca aagcaattga tcttctcgcg
    27061 cagggcgtcg atgcgtttat cttgcgcaac gttgtcctcg taatctgccg ccgttaagcg
    27121 tccgaacagc agcgggatcg ccaccatgta ctgaatgcag tggtcgcggt ctgccgggtt
    27181 attgagcggc ccttttttgt cgatgatgcg aatacaggct tcgtgggtgc gaatggtcac
    27241 tttttcgata tctgccgccg ttttgcctgc tgcctgcatc tgttcataga gcgtcatcgc
    27301 cgcttcaact gccgtctggg agtggaactc cgccgggaag gagattttga acagcacatt
    27361 ttccatgacg taggaaccgt acggacgctg gaagcggaat gactcacctt taaaggagac
    27421 gtcgtagaaa ccccacaccg gcgcggtcag ggctgacggg taacccattt cgcccgtttt
    27481 cgccatcagc gccagacgta ccgcgcggga tgtagcatcg cccgccgccc aggatttacg
    27541 cgtaccggtg ttcggtgcat gacgataagt gcgcagcgac tgtccgtcta cccatgccag
    27601 cgaaacggcg ttgagaattt cctcgcgggt caggccgagc atttcggcga ccacggcggt
    27661 ggaagccact ttcactaaca gaacgtggtc gagaccaacg cggttaaagg agttttccag
    27721 cgcgatgcag ccctgaattt catgggcttt gatcattccg gtcagcacct gtttcatggt
    27781 caacggcgct ttgccgctgg cgatcgcgtt gcgcgaaagc cagtccgccg ttgccagaat
    27841 gccgccgagg ttgtcggaag gatgccccca ctccgccgcc agccaggtat cgttgaaatc
    27901 gagccaacgg atcatcgcgc caatgttaaa tgccgcctgg acggggtcga gctgaaactg
    27961 agttccggga acgcgcacgc cgttgggtac gacggtgccg gggacaattg gccccagcag
    28021 ttttttacag gccggatatt cgagagcttc aagaccgcag ccgagcgtgt caagcaggca
    28081 gtaatgagcg gtgtcgtagg ctactctgga gctgatttcg tagttcatca cgtaatcgac
    28141 gatatcaacg atttcacgat caaattccgg gcggatgttg ttgatttgag ctgacattgg
    28201 gtacgtttcc ttattgttat tcgtagaggt ttactggcgc ttatccagcg cgacaaactg
    28261 gcggtcttcc ggtccaacat aattggcgga aggacggata attttgttgt cctgacgttg
    28321 ttcgataatg tgcgccgccc agccagtgac gcgggcgata acaaacagtg gtgtgaacat
    28381 ctcggtggga acacccatca tgttgtagga aacagcggag aaccagtcga gattggggaa
    28441 catctttttg ctctcccaca tcaccgtttc caggcgatcg gcgatgttgt acatcttcag
    28501 cgagccgcct tcctgcgaga gctgcttcgc cacacgtttg atcacctgat gacgcgggtc
    28561 ggcgatggta taaaccggat gaccaaaacc aatgaccact tctttgtttt ccacccgctt
    28621 gcggatatcg gcttcggctt cgcccggcgt ttcgtagcgt tgctggatct ccagcgacac
    28681 ttcattcgcc ccgccgtgtt tcggcccgcg cagtgcgcca atcgcgccaa taatggcgga
    28741 atacatatca gagccagtgc ccgcaatcac ccggctggta aaggtggaag cgttaaactc
    28801 gtgttcggcg tacagcacca gcgagatatg catcgccttt tcccagcttt gcgacggctt
    28861 ttcgccgtgc agcagatgca ggaagtgacc gccgatagag tcgtcatcag tttccggctg
    28921 gatgcgttcg ccgttgtggc tgtagtgata ccagtagaga agaatcgaac taagtgacgc
    28981 cagcagtttg tcggcaatat cccgcgcacc agaaacggtg tgcccctctt tttctggcag
    29041 cgtgcagccg agcgcggaaa cgccggtgcg cataacatcc atcgggtgtg acgccgccgg
    29101 taaggcttcc agcacggtac gcacgttagc cggtaaacca cgcagggctt tcagtttcgt
    29161 tttgtaggcg gcgagttcgt cacgggttgg cagtttgccg tggatcagca ggtgcgccac
    29221 ttcttcaaat tcacaatgtt ccgccagatc aagaatatcg tagccacggt aatgcaggtc
    29281 gttgccgctt ttacccacgg tgcagagcgc cgtattgccc gccggaacgc cggaaagtgc
    29341 caccgatttt ttcggtttaa tgacatgggt actgttttgc aggatcgttg tgtcgctcat
    29401 attgtcctcg tcattgttat catttgtagg gtcgagattg ttgtcggatg cgacgctatg
    29461 cgtcttatcc gacctacagt tcagttcacc tcgtaggcct gataagtcgc gtcagcggcg
    29521 catcaggcat cggttgtcga atacaaccaa cgggcgtttt tatttcacct gaccacgggc
    29581 aaacaggtcg tcgagcttct cttcgtactg gtagtagttg atgctttcgt acagctcgtt
    29641 gcgggtctgc atggtgtcga tgacgctttt ctgtgtgcct tcctgacgca ggatgttata
    29701 gacatgttca gcggcgcggt tcatggcgcg aaaagcagaa aggggataca gcgccattgc
    29761 gacatgggcg ctgcgtaatt cgtcggtggt aaacagcggt gttgcgccaa attcggtaat
    29821 gttggcgagg atcggcacct gcaccgcatc ggcaaactgg cgatacatgg cgagttcggt
    29881 aatcgcctcc gggaacagca tttcggcacc cgcttcaaca taggcctgcg cacgctcgat
    29941 cgccgcatcc agcccctcta ccgccagcgc atcggtgcgc gccatgatca caaaatcagg
    30001 atcggttttc gcatccaccg ccgcacggat ccgatccacc atttcctctt tcgagacgat
    30061 cgctttattc ggacgatgac cgcatcgttt cgcaccaacc tgatcttcaa tatgcaatcc
    30121 tgccgcaccg gctttaatca tcgatttcac ggtgcgcgcc acgttaaagg ctgaagaacc
    30181 aaaaccgata tccgcatcca ccagcagcgg cagcgaacaa acgtcggtaa tacggcgaat
    30241 gtcagtcagc acatcatcaa gagtagaaat accgagatcg ggcagcccca gcgatcctgc
    30301 cgccacgcca ccgccggaga gataaatcgc ctgatatccg gcacgctgcg ccagcagcgc
    30361 atggttagcg ttgatggtgc caacaatttg caacggggtt tctttgctaa gtgcagcgcg
    30421 aaacgcttta cctggagagt gtagagacat agcccatcct ttgttatcaa cttgttattt
    30481 gcgttgataa agacaaagca aggggtgtgc cacaattgac tgaataaaat taaatgttta
    30541 atttcaattt gttataaata aagtcatgaa gtaatccgca aatatgcgtt tcagttaacg
    30601 tttcaggcaa tgtttcaccg cgtttcattg caacaattat gaaacaagac taaacccaat
    30661 attcggtttc ttaactttgc ggtgcgctat ggcacatcca ccacggctga atgacgacaa
    30721 accggttatc tggacggtat ctgtaacgcg cctgttcgag ctgtttcgcg atatcagcct
    30781 cgagtttgat cacctggcga atatcacccc tattcagctt ggctttgaaa aagcggtgac
    30841 ctacatccgc aagaaactgg caaacgaacg ctgcgacgcc atcatcgctg ctggctctaa
    30901 cggcgcatat cttaaaagcc gcctgtcagt gccagttatt ttgattaaac cgagcggcta
    30961 cgatgtgtta caggcactgg caaaagccgg aaaactcacc tcttctatcg gcgttgtcac
    31021 ctatcaggaa accattccag cgctggtggc gtttcaaaag acctttaatt tgcgcctcga
    31081 ccaacgtagt tacatcaccg aagaagacgc ccgcgggcaa atcaatgagt tgaaagccaa
    31141 cggcaccgaa gcggtggtcg gcgcggggct gattaccgat ctggcagaag aagccggaat
    31201 gaccggaatt tttatctatt ccgccgccac cgtgcgccag gcgttcagcg atgcgctgga
    31261 tatgacgcgc atgtctctac ggcataacac tcacgatgcc acccgcaacg ccctgcgtac
    31321 tcgttacgtg ctgggcgata tgctcggtca atcaccacag atggaacagg tgcggcagac
    31381 tattttgctg tatgcccgct ccagtgcagc ggtgttgatt gaaggggaaa cggggacggg
    31441 caaagagctg gcggcccagg cgattcatcg ggaatatttt tcccgccacg atgcgcgaca
    31501 gggcaaaaag tcacatccgt ttgttgccgt taactgcggg gcgattgccg aatcgctgct
    31561 ggaagcggag ctgtttggct atgaggaagg ggcgtttacc ggctcgcgac gcggaggacg
    31621 cgccgggctg tttgaaatcg cccacggtgg caagctgttt cttgacgaga taggcgaaat
    31681 gccgctgccg ttgcagactc ggctgctgcg ggtgctggaa gaaaaagagg tcacccgcgt
    31741 cggcgggcat cagcctgttc cggtggatgt gcgggtcatt agcgccactc actgcaatct
    31801 ggaagaagat atgcagcaag ggcagtttcg ccgtgacctg ttttatcgac tgagtatttt
    31861 gcgtctacaa ctgccgccgc tacgcgagcg ggtggcggat attctgccgc tggcggaaag
    31921 ctttttgaaa gtgtctctgg cggcgctctc cgccccgttt tctgccgcat tacgccaggg
    31981 gttacaggca agcgaaacca tgctggtgca ctacaactgg ccgggcaata ttcgtgaact
    32041 gcgcaatatg atggaacgac tggcgttatt tttaagtgtg gaaccgacgc cggatttaac
    32101 gccgcaattt ttgcagctgc tactgccgga actggcgcgc gagtcggcga agactcccat
    32161 tccaggcttg ctgacagcac aacaggcact ggagaaattt aatggcgata agacagcagc
    32221 ggcgaattat ttaggtatca gccggacgac gttctggcgg cggctgaaaa gctgaactgc
    32281 cgaatgcagc gcgaacgctt tatccggcat aaatttcgat agatgatgat caccgataaa
    32341 cttacgcatc ggggatgttt acataggatt cagaattact tcttcttata aatatttgcc
    32401 gtgccgtgga tcttattgtc agtttgacca gaggtcagta ccaacacatc agcccctttt
    32461 tcatcggctt ttttgatcaa atcttctttc gcatctgcgg tcgacatttc attactggtt
    32521 gaaatatcac ctattttttc atattgcgat gcaacctttt caaattccgc tttggtcatt
    32581 aattctgcgg catagacatt ggtaacggca aacgctaacg caccgactaa cattttagag
    32641 attattttca tattacggct ccagagttgt tatcacacta tcacccaccc acattattgg
    32701 gttgattaat aattagttca gtcagggcat aaacgccagt aaccggaaat aagacacttt
    32761 gtgatctctc tcgcacccga ttatttttgg tagaaatatg atgaagttac ttcacttatt
    32821 tatggatgaa atcactatgg atcctttgca cgccgtttat ctgaccgtag gactgttcgt
    32881 gattactttt tttaatccgg gagccaatct ctttgtggta gtacaaacca gcctggcttc
    32941 cggtcgacgc gcaggggtgc tgaccggact gggcgtggcg ttgggcgatg cattttattc
    33001 cgggttgggt ttgtttggtc ttgcaacact aattacgcag tgtgaggaga ttttttcgct
    33061 tatcagaatc gtcggcggcg cttatctctt atggtttgcg tggtgcagca tgcgccgcca
    33121 gtcaacaccg caaatgagca cactacaaca accgattagt gccccctggt atatcttttt
    33181 ccgccgtgga ttaattaccg atctctctaa cccgcaaacc gttttatttt ttatcagtat
    33241 tttctcagta acattaaatg ccgaaacacc aacatgggca cgattaatgg cctaggcggg
    33301 gattgtgctc gcatcaatta tctggcgagt ctttctaagc caggcgtttt ctttgcccgc
    33361 tgtgcgtcgt gcttatgggc gtatgcaacg cgttgccagt cgggttattg gtgcaattat
    33421 tggtgtattc gcgctacgcc tgatttacga aggggtgacg cagcggtgag cagataattt
    33481 actctctacg tcatcaacct gatttgccga atcccaacca tcggcgatga tagcccccgt
    33541 aatgcgtggt ctttatttcc gataagttta cttgagtctt tagcttccca tttcagccac
    33601 tctcccctgt gtgggctttt tcattggtct gacccgtcct gaatagccaa acaatggcaa
    33661 tgttccactt tgcagggatg ataataagag gatctgattc agaaaagttt tgaaaagttg
    33721 gaccgccatc gaggcctcga accccgtacc atacaacaat cctggttgtc gtctgctctt
    33781 cctgatgagc taatggcggt ttttgatatg agccttacag catattttct gtcaggcttg
    33841 agtgcgatgc gtaagataca gaaaagaccc cacacaaaca tgatggaatt atgatttttt
    33901 tcatatgaat gtcaacccac acattcacaa aacatctgta tgagaacatt ttttatacac
    33961 catatagctg cgaaaccact actggtgctt ttttaagcga aactccgccg ttcacaacaa
    34021 aatatcggcg aaaaaggagg gggaatgcca tttggcatac agatatacag atgaaatgat
    34081 gacctgcttt gcgattcact ctctggtgga ataacaactt gcaggtggct ctcttaaata
    34141 aaaataaacg tcgcagataa taaagctctg attaaaccag acactatcag aaatcgctca
    34201 taactaacgt gtaaagtagt ggagttaatt taacaatctg aatttacgct ataggtttac
    34261 cactactttg atattatttc gctctcctta actaaaaagt taagtggagc gaagttaata
    34321 tgttaaacca caggatgttt attaattttt tcagtctgtt agtgtgcgat tatcgataac
    34381 aaaacgatat ttcacatcac cacgcagcat tcgctcatag gcttcattaa tttgatcggc
    34441 ccgaatcatc tctatatcag ccacgatgcc atgttcggcg caaaaatcga gcatctcctg
    34501 agtttctgga atgccgccaa tcatagaacc agctatcgcg cggcgtttca tgatcaggtt
    34561 gaaaacttcc ggcgatttat gcggtgtcgc aggcgcacca accagcgtta tggtgccatc
    34621 gcgcttcagc aaggtggtaa aatcgtcgag attatgtggc gcagctactg tattcaaaat
    34681 gaaatcgaaa ctcttcacat gagccaccat ctcatcggca ttgcgtgagt taacaacttc
    34741 atcggccccc agggcttttg ccgcttcgcg ttttgactca gaagtggtaa atgccaccac
    34801 atgcgccccc atcgcgtggg ccagtttaat ccccatatgt cccagaccgc cgatgccgac
    34861 cacgcccact tttttacccg gcccggcctg ccagtgacgt agcggcgaat acgtggtgat
    34921 ccctgcacac aacaaaggag ccaccgccgc cagctgctct tgcgggtgac gaatacgcag
    34981 aacatatcgc tcatgaacga cgatctgttg tgagtagccg cccagagtat ggcccggttc
    35041 atccggtgtc ggcgagttat aggtgccggt catgtaatca cagtagtttt ccagcccgtc
    35101 ttcacactct tcgcaatgtt tacaactgtc gacaatgcag ccgacaccga ccagatcgcc
    35161 cggcgcatgt ttttctacct gatcaccaac ggctaccacg cgccccacaa tttcatgacc
    35221 cggcacgcag gggtaaaccg tccccgccca ctccgaacgg acctggtgga gatcggaatg
    35281 gcagacgcca cagtaagcga tttcgatttt gacatcatgc ggtcccggtt cacgccgggt
    35341 gatatccatc ggttcaagcg gttgtttagc ggaatatgca ccaacagctt tgatcttcat
    35401 tgtgtttact cctgattagc tatgtgtatt tggcgatgcc tgatatgtca tcgcggcaaa
    35461 acgcgtccat tgaatatagc caatatttaa accacccgat ccagccccaa catgccgcga
    35521 gcctgaatga aacagagcag caacgttccc cataaggcca tcgtcaggta ggggcttata
    35581 ccgagaatat tgaagccgct ttcaataatt tgcagaagaa acagcgccag caccatacca
    35641 attatccgcc cgctgccgcc gtccggatta accccgccga gtactgccgc gaggattgac
    35701 accagcaagt acgattcgcc ataagaggcc ttagccgagt tgagtttcga catcatcaaa
    35761 aatgccgcta ccgcacaaag caaggcggaa atgacataca cccatatcag cacccgacga
    35821 gtgttaatac cggagtaaag tgttgctcgt tcgttagaac caatcagata aatggattta
    35881 ccgagcggcg ttttctccag caatatccac agccccagtg ccactgcggc aaacaaccac
    35941 atcggtagcg gaataccgaa ccactgtgca tgattcaacc acagcaccca tgagggataa
    36001 ttggcaatgg cgctaccgcc tgtcaccaga atatttatcc ctttgagcaa cgtcatcatc
    36061 ccaagcgttg caagaattgg cgacactcga ataccagcaa tcaatacgcc gttacataaa
    36121 ccgattatca ccgccgcgcc agcgcccgcc agtaacgtag cgacaaccgt agcaataccc
    36181 ggtggatact gagtcgccac ccacgccatc accagtgaac aggcgttagc agtggcaata
    36241 attgacaagt taatgccacc gcaaagcatg gtcaccgcca tagccaacgc cagaatgccc
    36301 agcacgggca tttgcgaagc gacggactgg aaattactta tcgaccagaa aatctccggc
    36361 atagtaatgc tgaacgcggc aacagttagc accagcaggc cgatcaagta aaactcaacg
    36421 ttattacgcc acgatttttt catagcagac tccgattctg atgctgactc cacgccgtgg
    36481 cactaatgct ggcaacgatg atgatgccgg tgatcaatgt ttgccagtaa gacgagactc
    36541 ccaataaatt tagcccgttt tgcatcactg ccaacaacac cacgccgagc aaagtaccgg
    36601 ttaacgtgcc gcgcccgccg agcaaactag tgccaccaag caccaccgca gccagtactg
    36661 tcagctcata acccagaaga gaatcggggg cgacagtcat caccgtccac gactgcacta
    36721 cacccgcagc accagacatc aatcccatat agccgtagac gaaaagttgc agtttcagca
    36781 cgctaaagcc gatgcgactg gcggattcac ggttcccgcc aagggcataa attttgcgcc
    36841 ctacactggt gaaattcatg ataaacgctg tcagcagcac caccgtaatc atggcgatca
    36901 gcggcagacc aaggccatag tcatagccgt cagcatcggt gtacttgaac aacattaccc
    36961 cctgctcaaa ccacggcgga aagtcgtaca gccacacacc tttactcaac cataacagca
    37021 ggccataaaa aatattcagc gtcgaaatgg tgatgatgat agaaggcacc cgtaggcgat
    37081 taaccagtaa ggcgttaatt aagccaagta aaatgccgat gccgcccgcc agtgcaaacg
    37141 cgacagcaaa gttaccacca attttttgta gcaacagcac catgccgtat tgcgcaatga
    37201 tggtcattgc cggaaacgaa atatcaattc caccggaaat caacaccaca aacaaaccac
    37261 aggcgagaat agtcaacatg gcatagttat tggcgagatc gtacaaatta ccgaatgtca
    37321 gaaactcgtc gctgcgccag gcaaggccaa caaacagcac cataatcagt aatgccagcc
    37381 agaattcatg acgttttttc agttcagcca tttaccacct ccgcaatcct ggcctgagaa
    37441 ctgctgtcag gcaggaagct atgggtgatc tgaccttttt gcattaccaa aatccggtgg
    37501 ctttgatacc acgcttcttc aatttcatcg cagatcatca ataccgcaat gccatgagcc
    37561 gccagatcgc tgataatgcc gtatattcct gctttgttgg cgatatcaac accgacagtc
    37621 gggctatcga gaatcaacag tcgtggccca atcgccagcc atttggcgat cgatactcgc
    37681 tgcgcgttac ccccggaaag cgtattcact gggagatgtg ggtcagagac tttgatggtc
    37741 aatgccttga ttagcctgtc caccaggtca cgcactttag cttcactcag aaagccgaag
    37801 cgatctttta ccttgtgaaa aacagtgcta atgatgttgt cttcaataga ttgcgccatc
    37861 accaaaccgc gcgacatgcg atcttccgaa acataaccaa taccgtggcg gatggcgtcc
    37921 tggttactat gcagtgtaac cggctgaccg ttgatgagga tctcaccagc atccggtcgg
    37981 gtcataccaa acagactcag acataattcc gtgcgccccg caccaagcag gccaacaatc
    38041 gacaccactt cgcccgcttc cacccgcaag ttaatatttt gatattcccc atgacgactt
    38101 aaattacgca cctccagcac tggggtgctg gattttccct gccacaactc tcgtacctga
    38161 tattcaaact cctggccggt cataaggaaa ccgagttgtt ttgtggtcat ctctgcagcc
    38221 ggaaaggtcc cgaccagctc accatctttc aacactgaaa tacggtcgga aacttccatc
    38281 acctcttcca gacgatgact gacgaagacc acgcagatcc cgcgttcccg caattgatgc
    38341 accacctgta ataacccttg cacttcctga tgggtcagcg aagcggttgg ttcatccatc
    38401 acgatcaacc gtgcgtcctg cgccagtgca cggcaaattg ccaccagctg acagcgggca
    38461 attgaaagct cagaaacgag cgtatccaat ggcaatgtga cgttaatact gatcattgcc
    38521 gcctgcgcca cctcacgcag acgacggcgg ttaacaaaca gaccgtggtg atagtgattc
    38581 acggcgatat tctcccagac actcaggtta ggaaataaag agaggtcctg ataaatcacc
    38641 tgaatcccct gcgccaccga agcggcgggt gtcagctttg accagttttt cccaccaagc
    38701 gtaatttccg ccccctcatc cgggcgataa acaccggaca taattttaat caacgtcgat
    38761 ttaccacagc cgttttgccc ggcaaggcag tgcacctcac ccgacataaa gtcgagagaa
    38821 acatcgcgca gcgctcgcgt ggcatgaaac gttttgttga tatgacgaag ggaaaggaag
    38881 gtttccataa catcccctgg caataagaat cggggctttc gccccgaaga aatggattaa
    38941 taaagtgagt cgatattgtc tttgttgacc agtaatacct tatggaaacg gataattcgc
    39001 ttatccatat ctacgtcggc cttacccaga ttctgcattt ctaatccagg tttgatctct
    39061 tcacccttca gcaacgtgct ggcgacggcc gcgagtgcgt aacctgcaga ggccggatcg
    39121 taagtaatcc cttcggtgat atcaccactt ttaatcagtg atgccgcctg tgaagggatc
    39181 atcatgccat agactgcgac tttatttttc gcccgtttct ctttcactgc acgtcccgcg
    39241 ccaatcggac cgtttgaacc aaaggagaca accgctttca agtcaggata ggttttcatc
    39301 aggtccagtg tagtacgacg tgagtcatcc acactctcgg caaccggcat gcggcgggta
    39361 acttcatgca tatccgggta atgctctttc tggtatttca ccagcaagtc agcccataag
    39421 ttatgctgcg gcacggtcaa actacccacg taaatcacat agccgccctt gccacccatg
    39481 cgtttcgcca tatgttcaac atattcagcg gcaaattttt cgttatcaat gatttcgata
    39541 tcccagttag cacttggctg accgggggat tcgttggtca gaaccacaat tccggcatct
    39601 cgcgcttttt tgaataccgg ttccagcacg ttggcatcgt ttggcacaat tgtaattgca
    39661 ttaaccttac gggcgattaa atcctcaata attttaacct gttgcggagc atcagtactt
    39721 gaaggtccca cctgtgaggc attaacacca aaggctttac ccgcctcgac cacaccttcg
    39781 cccatgcgat taaaccacgg cataccatcg actttagaga tattcaccac gactttttcc
    39841 gctgcctgga gcggcgcaga aattagcgca gcgcctaata acagcgaaga caccatattg
    39901 ataacaaaac gtttattcat catttcagcc tcattgcagg gtagtcagga agtgttcgcc
    39961 caaattagcg cgctggagag aataagaatt ttcgaatatt tttatgcaga ataaacagcc
    40021 ctatttcagg cagctattaa aaatttggtt ataaatatgg acaaaacggg cagcaataat
    40081 aagtgtgaca ttcatcacgt aaaaaatagc aaaataaaca aaattagcga tattttatgt
    40141 ttgtgagctt atttcgcgta gaaaaatagt tatctacaca acgtcaataa gggcatattc
    40201 ttcaaagata acgtccattt cgggtgaata gcatccttct gcctggaaaa aacgacggtg
    40261 ttcattacgc caatattcaa gactgaggtc accttcacct tccttacggg caagctccgc
    40321 ggtgacatca gaaaagcgca gtaaatgcag gccaatcact ctgataacac agacaggctc
    40381 accacggcta ttttctacaa tcttatactc accaatcatc ggaaatgcgt tgtcttcaat
    40441 acatcccgcc agcgaaccac aggaagctgt tttaacacct gtcgcaatca acgttgcgag
    40501 gtgatcggcc aacgctgctg aatcgccaaa agtacaaaag gaagcatggg gatatttttg
    40561 tgagattttt tcttttatgg tcatgagaat attttcgcga ttacggaaaa aaatcacatc
    40621 ttaattactg ttgataacca tcagcaagag gatcattggt ttttctatgt ttaatctttt
    40681 ttatccgaag ccggtcacat ttatggcttg ataaatacct ttgttaaaac tcacttttgt
    40741 gtatttctct caaataatcc cctggaaatt atgccgggag attataaagt tcaggagcat
    40801 tcattagcaa accattgctg ccccactccc tggggcttta aatctcttac gattcaccat
    40861 tgcaggcgtt tcaaatgcgt atcaacctgc cacactcccc cacaccagtt gccctttatg
    40921 gaacgttgcg gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggtgtccac
    40981 cagcacaaaa ctggcgtcat cctgcgcttt tggccacacg cgctcgcctt tttcatttag
    41041 cggcaataca tcaccggtgg ctaaaaacag cgagcgggat aaattctgtt cgttagggcg
    41101 aatatagagc tgcgcgtaca gattggcttt ttccagcatg tcgcccaggc cgtagggtga
    41161 ccagtggtcg ataacgctgt cggtgccggt catcactttt acgcctttgt cgtgcagctg
    41221 tttgagcggc atatgcaacg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc
    41281 caccatccgg tgcgccagtt tatctacctg ttgttcgttg agcgttgcca gcgcaaaggc
    41341 gtggctgatg gtcagcttgc cttttagctg cggcgttttc tccactgttt caaccatata
    41401 attgatggct gccacgcctg ccggcgtggt ttcgtgcagg tgaatatcga cgcctttgtc
    41461 atagtccagc gcaatctgga acatggtgtc gagggatttt tccatcgcgc catcaacact
    41521 ggtcgggtcc agcccgccga cgtaatgcgc ccccgcctgc atcgcttcgc gcattaaggg
    41581 ttccgatttc gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc
    41641 cgcctgacgt cgcgccagca ccgcctgcaa attttgcaga tttttcaggc cggaaaccgg
    41701 ttcgatattg cagtggctgc gggcaatggt ggtgcctttc gactgcaata aatcgatcag
    41761 tttttccgcc cgttcctgcg tgtacggttg cagttccggc agcattttct gctcgagttt
    41821 gatcatgtcc tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta
    41881 gaaggttttg tcgagatgaa tatgcatgtc gcgggtggtg ggcagcatca gcttaccgcc
    41941 agcgtcatag tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcaacaat
    42001 tttaccgttc tgaatttcca cagtctggcg cgccgtgcgg gtctgcaccg ccacgccatt
    42061 ttcgtagtca aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat
    42121 tttcatggtg ttgttcgctt cgcagtttag ggtgccagct ccccccgcat gggcgagcgg
    42181 cactgaggga ccaaacagcg cggcggcggt aaccatctta ccgctctggc tcagaaattc
    42241 acggcggcta ttgttttctt tcatcttacg tccttaggtt ttaataatat gggtgcccgt
    42301 ttcgccgcgt agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc
    42361 ttgttccagg aacatcaggc tggcgatgat ttttggcaac atgctgccag gtgggaaatg
    42421 cccttcctgc atatagcggg tcatggtggc aatatctacg cgatccagcg cctgctgctg
    42481 cggtttgcca aagtgaatac acactttttc gacgccagtg gtgatcacca gaatgtcggc
    42541 gtgaatttcg cgagccagta gcgcggtaga gagatctttg tcgataaccg cgtccacgct
    42601 ttggtaatcc cccgcgtcag tacgcactac cggaattccg ccgccgcccg cgccaatcac
    42661 cacaaagcct tgttggatca gcgccttaat ggcaggtgct tcgacaatac gtttcggttc
    42721 cggcgaggcg accacgcggc gatagccccg tccggcatct tcaacaaaac gccagtcagg
    42781 gtttgccttt tgtagttcgt cacgctggct ttcactaaag aattcgccga tgggcttggt
    42841 gggatgggca aaccccggat cgtttttatc cacttccacc tgagtcacca cggtgacggc
    42901 ttttttctcg ccgtgacgcg ccagtcggtt gttcagcgcc tgctggatca gatagccaat
    42961 gccaccttgc gtatccgcca cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc
    43021 gtgggcaatc tccgcgcggc gtaaatccag cccgacctgc ggcccgttgc cgtgggtcag
    43081 cacaatgtcg taatcggaag ccagcatttc cagcaccgta tcggccacgg ctttcaccgc
    43141 ctccgcctga tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat
    43201 ggcaacgacc acaagttctt tcatgttgtt atgtccgcta cagagggtga tggggttatg
    43261 accaacgcca gactgcctga tgcgctacgc ttatcaggcc tacagattct tgcgccactc
    43321 gtaggccgga taagacattc atgtcgcatc cggctgtggt ggccaatgcc tgaagcgcca
    43381 cgattatcag gcctacagat ttccgcgcca ttcgtaggcc ggataaggca ttcatgtcgc
    43441 atccggcata aacatgtaac tcagacgccg taacgttcgc accagccaaa aatggctttc
    43501 tcgaagcagg cgaagggtgg atgcacaatg cccgcgccaa tcatgccgac gcccgcatct
    43561 ttatgggcga tggcggtgtt gatcactggc agaatgccgc tgctacccac gcgggtgatg
    43621 tcgattgccg acggcacgcc cataaaaccg agcagcggaa tggtgacgtt ggggttttcg
    43681 ccaagggtga tttcacgcat ctgacgggag aaatcgatag cctcttccac cgtgcccccc
    43741 accagcgcga cgatcgcggg cgcggtcgcc atcgcaaatc cgccaatacc gtaggtttcg
    43801 gtgatggcgc tgtcgccaat atccagccct gaatcttccg gcttataacc ggcaaacatc
    43861 gggccgatga cctgctgcgc cgggccggta aaccactgcc ccggcaggcc gctgacccgc
    43921 aggccgaact cgacgccgtt acgcgccatg gtggtcacca cggtgctgta ttcgatgccg
    43981 tgcgccgcat ccatcgccgc tttacacatc gccatccacg tcgggccgga gaagtagtcg
    44041 ctgctggcga caaactcaaa cacttcgcgc tgttgctcga cggaataacc tgcctgaata
    44101 atccccggcg tcagcgcctg aatcagcagc gtcgtcccgg cgttattgcg gttatggcat
    44161 tcatcgccca tatgcagcgc ctgcgccagc attaagcgca gatcgatttc gccgataatt
    44221 ttcatcgcgt cgcgcagcat gggtccctgc acatcgcgca tccagttgag gcggtcgatc
    44281 acgctctggt cgttagcgcc catacgcaaa atcttcgcca tctgctcgct catgttggta
    44341 taagcgatgt tgccataggt tttgtttttc acgatgtgca taaacatcga ggccgaggtg
    44401 acgcccgcca tcgatcccac acagtcgtgt tcgtggcacg acgagaaggt gatctccccg
    44461 gaagccgcca gctcagccgc ctcgtcgaga tctttcgcca gtccttcaaa caccagcgct
    44521 ccggtgaccg cgcctttcat cgcgccgcac attttttccc aggtgaccgg tggcccggcg
    44581 tgaagaatgg ttttcgccgt catgcccggc accacgttaa tcgcctgatc aaagccgatt
    44641 agcaccggat gcgactggat aatgcgctcc agcgcctgct ggttagcggc ggcgattttg
    44701 tctgccagcg gcgaatcggc aatgttatcc agcgcctgca ccacctgcat attgccctgc
    44761 cccggcggcg tccagtcgag ttgggtcact tctacatgct gctttttcag atcatcgcta
    44821 aacatggcga taccgacgtt aataacattc aacggttggc taaacagtga ctggctcatc
    44881 aggcttcctc ccctttgcag ataaattcac gcgccagcaa tccggtattg gtgctgctgc
    44941 tcgccagaat cactccggca tcaagcagca tctggctttg ttgttctaat gatggcgtat
    45001 cgagatcggt gcccagcaca taggcgagaa tgatcaactc gcgtccttcg gcagcggcga
    45061 tcgctttcgc ttctctgatc gcctcgatgg tggaaccgac cggatcttca tgcgatccaa
    45121 atccgagcac aaaatccatc acgatcaccg ccacttctgg atcgcgcgct tcttcgatca
    45181 agcggctgat acggttggtc gggtcaatca ttggatgcgg cttgccgttg gtgaagtcat
    45241 catcgccaaa atcgaggaag gtgtgtttga tgctgcggtt gatatcttgc aggcggaatt
    45301 caggatcggg ctggatattg ctgtagacat cgccatgttt ttccatcacc gcgaacaggg
    45361 tttcgtcaca cagcgtaccg ccgcagaaca gaccacgaat gtatttctgc tgcggttgca
    45421 gacgcgcacg cacatccgca atcaacggct ggttaagcgt atgcaggtcg agatgttctt
    45481 gtttcacgcc ggagagcatc accgctttca atgccgcctc tttgctgccg cgagcaaact
    45541 gtagcccctg ctcatccact ggcgtttcgc cacgaccgag gaagcaggcc accactggct
    45601 tgcggcaggc gcgtgcgcgt tccagcactt tgcgggccac cgcaggcgca ggcggtttgg
    45661 agataagcac aatgatttca gtttgcggat cgttttccag catcccgatg gcatcgagca
    45721 tcatcaggcc accgattttc tcgctcaggt cgcgcccgcc ggtgccgatc agttgtgaaa
    45781 cgccgccacc aaattcatgg atacggacgc tcaattcctg gctgccggtg ccagatgcgc
    45841 caacaatacc gatgttgccg cgacgcacgg cgttaccaaa gcagagcgcc gcgccgttga
    45901 taatcgccgt gccgcagtct ggccccatca tcagcagccc tttttcatgg gccaactgtt
    45961 tgagcgccag ttcatcttca actgagacat tatcggaaaa cagcatcacg ttgaggtcat
    46021 tttgcagcgc ctgacgcgct tcgcgagcgg caaacagacc gttgaccgaa atcactgcca
    46081 ggttactttc cgggatatgc tttttggcgc tggcaatagt gccgtaacgc gcctcgtgcg
    46141 agccgctttg cgctttggtg ttgaacagtt cttcaatctc cgccagcaac tgctcgttgt
    46201 ccacacccga tttaccattg atgacaatca tcaggtcgcc gtttttcgcc tgctccagct
    46261 ccggcgtcag cagtcctaaa ttcttcaaca cgcctttgtt catttcggtc gccatcgcca
    46321 caaatgcctg ctcgacgccg tcgagtttat ttgcacgcgt ggagatagac atcagcgaga
    46381 cagaatcaaa ataggtattc ggtttaatga ctattttaac tgacataatt ccctccgtag
    46441 gtttgactca gcgcacaacc atcggccatg ccatacagca tgtcgcagcc ggaactggag
    46501 ccaatatgtt taattttttc tattgcctga gttgcgttcc cagggatgtc ataaataatg
    46561 ttgtgaataa aacgatgaat attttcccgg cagcgttgtt gtaatgcggc ttccagcgtg
    46621 atggcactta ataacgtggt attatttctg ccgcgttgca aaccgagata aaattcctct
    46681 ttgtattttt ccgccggatg tccggaaata aataaaataa gtaccagtcc ggtcagataa
    46741 tcatcggctg atggcgtaag gccaatccct aagcccatca tactggcgac tgctgaggcg
    46801 atattttgtt tatcgtttac ggcctgaata actgcccgtc gtttaatatg tagttcttta
    46861 tttaacgcct gataaaacgg attatcgccg cgataattaa acagggtttc attttccccc
    46921 aggtgctgat gaataatgtc gtgccactgt tgccaggcga taaggtgaaa attcacagcg
    46981 gtcatttgca aggttgggga cagccaacgc cgacaacgac tcatctctat atgaagatgt
    47041 tgaccaaccg taatgccttg atcgtcaaac tgaaccttat caccatgccg gaacagacca
    47101 ttaaagtgag tgagtgccaa ccgacaactg tttggcgcat tgtcgtactc ttcacaaagc
    47161 agcgttagca actgctggcg agccgggagt gcgatattta ccgctctggc aaagacctgt
    47221 tcaacctgtc cttgcccccg ctgcgccaga aaatcagcat ccgcagttag cgcccacaca
    47281 caccgttttt ctggtgtgtt gatagcaagc ctcccggttt atgcacctgc ggcgatcagc
    47341 aactgcgcaa tctcttcaaa gccccgttcc cgcgccagtt ccagcggcgt tttgccatat
    47401 ttatcggtca gatgcgggct ggcaccgtgt tccagcaata actggacaat cgcctgctgt
    47461 ttaatcccgc catcgttaag cacaatcgct tccagcaacg gtgtccagcc aacatggtta
    47521 gtctggttaa cgttaatctc cgtatttgcc aaaagctctt ttacaatact taaatggcct
    47581 ttttcacagg caggcgtcag gccgacaccg ccaaaacggg ttacgcaatt aagatcgggt
    47641 ttagccggta aaataattcg tagtagcgtt aaatcatcgt tcagacagct aattaaaaaa
    47701 ggatttaaac aggtatgatc ttgtttatta atattcgctc cggcgtcaat taatgcctga
    47761 acgcaagcat attgctgata taaacttgcc agcgtaattg ccgttttccc ctgacgatcg
    47821 caggtattaa tatcgacacc cagcgcaagg cagattttta ctttatcgat atccccctgt
    47881 tgcgcagcca ataaatagtc tgcaggtaga ttttttatag tcattcacat tcacctggtt
    47941 tttgtggctg ttttcgatga attgagaata gcaacgataa tctgataaaa gaacccgctt
    48001 aaatttaatt cgccgtattt cgcctttttg ttgaatgatg ttcaacaatc ttttcgccgt
    48061 gtgcgatagt gcaatatttg tttctttgac ccgataccct ttcactcaat caggttttat
    48121 ttctctatag tctgcacatg taagagtagc tttcgtccat gtctaaccag aggggaacaa
    48181 gatgaacgga ctcacggcaa cgggtgtcac cgtcggtatt tgtgcagggc tttggcagtt
    48241 agtgtcttca catgtcgggc tctctcaggg atgggaatta ttaggaacca ttggcttcgt
    48301 cgccttttgc agtttttatg ccgcaggcgg cggtaaatcg gggtttatca aaagcctggc
    48361 ggttaattat tccgggatgg tgtgggcgtt tttcgccgcg ctggctgccg gttggctggc
    48421 cccggtgagt ggactgtccg cgttctgggc gagcgtaatt acgactgtac ccttctcggc
    48481 ggtggtggtc tggcaagggc gtttctggtt actgtctttt attcccgggg gctttctcgg
    48541 catgacgcta ttttttgcca gcgggatgaa ctggacggtg acattactgg gttttctggc
    48601 gggtaactgc gtggggataa tttcagaata cggcggacaa aaattaagcg aagcgacgac
    48661 taaacgcgac gggtattaaa agttattcaa aaatgattat ctatctttaa ccgatacctc
    48721 tacagtcatg gctggagaaa ttcatattag ataatggcgg gaatggttta atgaactcaa
    48781 tttttaccga agagaatctg ttggcattta ctaccgctgc gcgttttggc agcttcagta
    48841 aagccgcaga agagttgggt ttaaccactt ccgccattag ctacaccatt aaacgtatgg
    48901 aaacagggct ggatgtggtg ctgttcactc gcagtacccg cagcattgag ttaacggagt
    48961 ccgggcgtta ttttttccgc aaagctaccg acctgctgaa tgattttcac gccattaaac
    49021 gcagtatcga tactatttcg cagggcatag aagcgcgtgt gcgcatttgt attaatcagc
    49081 tgctttatac gcccaaacac accgcgcgct tattacaggt gctgaaaaag cagtttccta
    49141 cctgccagat taccgtgacg accgaagtgt ataacggcgt ctgggatgcg attattaata
    49201 atcaggcaaa tatcgccatc ggcgcgcccg acacgctgct ggacggcggc ggcattgatt
    49261 acacggagat tggcgcgatt cgctgggctt ttgccatcgc cccggatcac ccattagcct
    49321 ttgtgccgga gccgatcgcc gaaagccagc tgcgtctcta ccctaatatc atggtggagg
    49381 acaccgcgca tacgattaat aaaaaagtgg gctggttgct gcatgggcag gagtcaattc
    49441 tggtgccaga tttcaacacc aaatgtcagt gccagatttt aggtgaagga attggttttt
    49501 tgccggatta catggtccgt gaggcgatgg cgcaatcctt gctggttacc cgacaaatcc
    49561 ataacccgcg ccaggattca cgcatgttac tcgcaaccca gcactctgcc accggtcagg
    49621 tgacgcagtg gattaaaaaa caatttgcgc caaatggcat attaacgggg atctatcagg
    49681 atttactgca tcgggagaat taatcgctgc caacctcccg atgcgctgtg aatagcggtt
    49741 tacatcaacc acctgctttc attacccatt cagagataaa tttattaccc ggtactggcg
    49801 gagagtacaa atagccctgt aaaaagtgaa cgcctttacc gatcattaaa tccgcctgct
    49861 cctgggtttc taccccttcc gccacgatac tcagaccagg cttacgcgcc agttcgacaa
    49921 tattgtccac aatatgaccg gagatttcat cgacactcgc catttgcaca aacgacttgt
    49981 caattttaat aaaatcgacc gggaacgcct gcaagtaacg ataggtcgca taacccgtac
    50041 caaagtcatc cagcgcaaag gtaatattgt gctgatgaag gctgtcaaat atcgctctgg
    50101 cttctggcgt taccggaata ggattacgtt ccgtcagctc aagaaccagc ttgattttat
    50161 cgttacctaa tttcttaacc aggttcagac actctttttc aaatcccgca gccaaaaaac
    50221 agcccgccga gacgttgata ccaatatgga aattgtccgg cagcaaatgt tttaccggca
    50281 tcagaatatc cgcagtctgt ttcatcagct ggcgagtcat tatgacaata agaccggatg
    50341 actccgccag aggaataaac tgatccggtg ggatcattcc cgtttgtgga tgttcccagc
    50401 ggacaagcac ttcacagcct gtcagtaccc cagtctgcgc gcagaaaacc ggttggatcc
    50461 acggtttaaa ctcgtggttt tccaacgcca gactgatggc ttcaggcgtg acgatatggt
    50521 gatagtgatt atcattaata tatttgtgac tattattacc tgtggcggat atgatctccg
    50581 gattgtactg aaagaaaata tcccgccaga aagtaatatc gctctgaatc cccagtttct
    50641 caaagagctg tttcttttga tgtgaaatgg tctttgcact gcgatttctg tactgtgata
    50701 tttctgttac agacattccc tgcatcagca gcttgagtag cagtcgttct ttctcggata
    50761 cgctaccagg caatgacaaa tgaaccgttg taccgaactc ttgcagaaaa acacgaaaat
    50821 cacatgaatt catgaacaca ccttttatct tttatcgctg ttcagtatga gtgattacct
    50881 gtggattcct gagcacacat aaggggtaac cataaacatt agcgatgcca tacgtataac
    50941 atcccacatc actatcaaga agattatctt tacctctgat gctcgctcaa aaacagttac
    51001 gctaaatacc ttaaaaaggt aatcacaagt caaactcagg agataatcac gcaagaatgc
    51061 aatattaaaa ttcagatact catttttggt aataaagaag tagttggact atttcgatat
    51121 ttataatttc ccactacgta ttgacgccca ttcatgagta aaggcgcatt atatgaattt
    51181 aacaatccag atcaactgaa aatacctcaa cctcataaac acatagcgtc aacattcaat
    51241 gacataatga gtaaagatgt tggttatgca tacacgtcat tactctatgc ctatccctta
    51301 acaacctgct ttttaagact gaatccattc agcaatagat agccaacatt ataacaatga
    51361 caat
//
LOCUS       DADSZD010000012        50183 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.012, whole genome shotgun sequence.
ACCESSION   DADSZD010000012 DADSZD010000000
VERSION     DADSZD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.012"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            185..1132
                     /gene="cyoA"
                     /locus_tag="J1Z28_000625"
     CDS             185..1132
                     /gene="cyoA"
                     /locus_tag="J1Z28_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBA7715486.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1154..3145
                     /gene="cyoB"
                     /locus_tag="J1Z28_000626"
     CDS             1154..3145
                     /gene="cyoB"
                     /locus_tag="J1Z28_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBA7715487.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3135..3749
                     /locus_tag="J1Z28_000627"
     CDS             3135..3749
                     /locus_tag="J1Z28_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBA7715488.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKVFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVVSWL
                     ALTWLFGAGFIGMELYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3749..4078
                     /locus_tag="J1Z28_000628"
     CDS             3749..4078
                     /locus_tag="J1Z28_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBA7715489.1"
                     /translation="MSHSNVSGGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4090..4980
                     /gene="cyoE"
                     /locus_tag="J1Z28_000629"
     CDS             4090..4980
                     /gene="cyoE"
                     /locus_tag="J1Z28_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBA7715490.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5129..6493
                     /locus_tag="J1Z28_000630"
     CDS             5129..6493
                     /locus_tag="J1Z28_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715491.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMANAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(6621..7112)
                     /gene="yajQ"
                     /locus_tag="J1Z28_000631"
     CDS             complement(6621..7112)
                     /gene="yajQ"
                     /locus_tag="J1Z28_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBA7715492.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7280..8191
                     /gene="panE"
                     /locus_tag="J1Z28_000632"
     CDS             7280..8191
                     /gene="panE"
                     /locus_tag="J1Z28_000632"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBA7715493.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTATWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8154..8744
                     /gene="yajL"
                     /locus_tag="J1Z28_000633"
     CDS             8154..8744
                     /gene="yajL"
                     /locus_tag="J1Z28_000633"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBA7715494.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(8798..10246)
                     /gene="thiI"
                     /locus_tag="J1Z28_000634"
     CDS             complement(8798..10246)
                     /gene="thiI"
                     /locus_tag="J1Z28_000634"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBA7715495.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10452..10694
                     /gene="xseB"
                     /locus_tag="J1Z28_000635"
     CDS             10452..10694
                     /gene="xseB"
                     /locus_tag="J1Z28_000635"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015571292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBA7715496.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            10694..11593
                     /gene="ispA"
                     /locus_tag="J1Z28_000636"
     CDS             10694..11593
                     /gene="ispA"
                     /locus_tag="J1Z28_000636"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBA7715497.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            11618..13480
                     /gene="dxs"
                     /locus_tag="J1Z28_000637"
     CDS             11618..13480
                     /gene="dxs"
                     /locus_tag="J1Z28_000637"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBA7715498.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDATGMEAKIKAWLA"
     gene            13535..14509
                     /gene="yajO"
                     /locus_tag="J1Z28_000638"
     CDS             13535..14509
                     /gene="yajO"
                     /locus_tag="J1Z28_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBA7715499.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDSANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLLRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14563..15081)
                     /gene="pgpA"
                     /locus_tag="J1Z28_000639"
     CDS             complement(14563..15081)
                     /gene="pgpA"
                     /locus_tag="J1Z28_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBA7715500.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDHNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15059..16036)
                     /gene="thiL"
                     /locus_tag="J1Z28_000640"
     CDS             complement(15059..16036)
                     /gene="thiL"
                     /locus_tag="J1Z28_000640"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBA7715501.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(16114..16533)
                     /gene="nusB"
                     /locus_tag="J1Z28_000641"
     CDS             complement(16114..16533)
                     /gene="nusB"
                     /locus_tag="J1Z28_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBA7715502.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(16553..17023)
                     /gene="ribE"
                     /locus_tag="J1Z28_000642"
     CDS             complement(16553..17023)
                     /gene="ribE"
                     /locus_tag="J1Z28_000642"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBA7715503.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17112..18215)
                     /gene="ribD"
                     /locus_tag="J1Z28_000643"
     CDS             complement(17112..18215)
                     /gene="ribD"
                     /locus_tag="J1Z28_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBA7715504.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQAQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(18219..18668)
                     /gene="nrdR"
                     /locus_tag="J1Z28_000644"
     CDS             complement(18219..18668)
                     /gene="nrdR"
                     /locus_tag="J1Z28_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBA7715505.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            18819..19358
                     /locus_tag="J1Z28_000645"
     CDS             18819..19358
                     /locus_tag="J1Z28_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBA7715506.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            19657..20541
                     /locus_tag="J1Z28_000646"
     CDS             19657..20541
                     /locus_tag="J1Z28_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBA7715507.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(20579..20926)
                     /locus_tag="J1Z28_000647"
     CDS             complement(20579..20926)
                     /locus_tag="J1Z28_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBA7715508.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21056..22027)
                     /gene="secF"
                     /locus_tag="J1Z28_000648"
     CDS             complement(21056..22027)
                     /gene="secF"
                     /locus_tag="J1Z28_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBA7715509.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22038..23885)
                     /gene="secD"
                     /locus_tag="J1Z28_000649"
     CDS             complement(22038..23885)
                     /gene="secD"
                     /locus_tag="J1Z28_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBA7715510.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(23913..24245)
                     /gene="yajC"
                     /locus_tag="J1Z28_000650"
     CDS             complement(23913..24245)
                     /gene="yajC"
                     /locus_tag="J1Z28_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBA7715511.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24268..25395)
                     /gene="tgt"
                     /locus_tag="J1Z28_000651"
     CDS             complement(24268..25395)
                     /gene="tgt"
                     /locus_tag="J1Z28_000651"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBA7715512.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(25450..26520)
                     /gene="queA"
                     /locus_tag="J1Z28_000652"
     CDS             complement(25450..26520)
                     /gene="queA"
                     /locus_tag="J1Z28_000652"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBA7715513.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            26613..27194
                     /gene="acpH"
                     /locus_tag="J1Z28_000653"
     CDS             26613..27194
                     /gene="acpH"
                     /locus_tag="J1Z28_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBA7715514.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFINLNNYLWSERWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(27199..29016)
                     /gene="malZ"
                     /locus_tag="J1Z28_000654"
     CDS             complement(27199..29016)
                     /gene="malZ"
                     /locus_tag="J1Z28_000654"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBA7715515.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTSEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVAGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29172..30545)
                     /gene="proY"
                     /locus_tag="J1Z28_000655"
     CDS             complement(29172..30545)
                     /gene="proY"
                     /locus_tag="J1Z28_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBA7715516.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(30621..31940)
                     /gene="brnQ"
                     /locus_tag="J1Z28_000656"
     CDS             complement(30621..31940)
                     /gene="brnQ"
                     /locus_tag="J1Z28_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBA7715517.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(32347..33642)
                     /gene="phoR"
                     /locus_tag="J1Z28_000657"
     CDS             complement(32347..33642)
                     /gene="phoR"
                     /locus_tag="J1Z28_000657"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBA7715518.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(33700..34389)
                     /gene="phoB"
                     /locus_tag="J1Z28_000658"
     CDS             complement(33700..34389)
                     /gene="phoB"
                     /locus_tag="J1Z28_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBA7715519.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            34579..35781
                     /gene="sbcD"
                     /locus_tag="J1Z28_000659"
     CDS             34579..35781
                     /gene="sbcD"
                     /locus_tag="J1Z28_000659"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBA7715520.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHTPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            35778..38921
                     /gene="sbcC"
                     /locus_tag="J1Z28_000660"
     CDS             35778..38921
                     /gene="sbcC"
                     /locus_tag="J1Z28_000660"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBA7715521.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEDEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSTALAHTRQQIEEVNTRLQSTMALRVSIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQETRIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     ITAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFEDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAMK"
     gene            39047..40231
                     /gene="araJ"
                     /locus_tag="J1Z28_000661"
     CDS             39047..40231
                     /gene="araJ"
                     /locus_tag="J1Z28_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBA7715522.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(40500..41408)
                     /gene="mak"
                     /locus_tag="J1Z28_000662"
     CDS             complement(40500..41408)
                     /gene="mak"
                     /locus_tag="J1Z28_000662"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBA7715523.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYLQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            41533..42444
                     /gene="rdgC"
                     /locus_tag="J1Z28_000663"
     CDS             41533..42444
                     /gene="rdgC"
                     /locus_tag="J1Z28_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBA7715524.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(42525..42605)
                     /locus_tag="J1Z28_000664"
     CDS             complement(42525..42605)
                     /locus_tag="J1Z28_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715525.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(42602..42931)
                     /locus_tag="J1Z28_000665"
                     /pseudo
     CDS             complement(42602..42931)
                     /locus_tag="J1Z28_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(43088..43372)
                     /gene="ppnP"
                     /locus_tag="J1Z28_000666"
     CDS             complement(43088..43372)
                     /gene="ppnP"
                     /locus_tag="J1Z28_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBA7715526.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(43444..44121)
                     /locus_tag="J1Z28_000667"
     CDS             complement(43444..44121)
                     /locus_tag="J1Z28_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBA7715527.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(44379..44570)
                     /gene="yaiA"
                     /locus_tag="J1Z28_000668"
     CDS             complement(44379..44570)
                     /gene="yaiA"
                     /locus_tag="J1Z28_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBA7715528.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(44620..45144)
                     /gene="aroL"
                     /locus_tag="J1Z28_000669"
     CDS             complement(44620..45144)
                     /gene="aroL"
                     /locus_tag="J1Z28_000669"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBA7715529.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(45327..45785)
                     /locus_tag="J1Z28_000670"
     CDS             complement(45327..45785)
                     /locus_tag="J1Z28_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBA7715530.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            45905..46714
                     /gene="proC"
                     /locus_tag="J1Z28_000671"
     CDS             45905..46714
                     /gene="proC"
                     /locus_tag="J1Z28_000671"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBA7715531.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(46731..47846)
                     /gene="adrA"
                     /locus_tag="J1Z28_000672"
                     /gene_synonym="dgcC"
     CDS             complement(46731..47846)
                     /gene="adrA"
                     /locus_tag="J1Z28_000672"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBA7715532.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNALPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(47948..48268)
                     /gene="psiF"
                     /locus_tag="J1Z28_000673"
     CDS             complement(47948..48268)
                     /gene="psiF"
                     /locus_tag="J1Z28_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBA7715533.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(48387..49802)
                     /gene="phoA"
                     /locus_tag="J1Z28_000674"
     CDS             complement(48387..49802)
                     /gene="phoA"
                     /locus_tag="J1Z28_000674"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBA7715534.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQSDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(49903..50163)
                     /gene="iraP"
                     /locus_tag="J1Z28_000675"
     CDS             complement(49903..50163)
                     /gene="iraP"
                     /locus_tag="J1Z28_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBA7715535.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        12185 a        13396 c        12698 g        11904 t
ORIGIN      
        1 ctttcgttat aacgcccttt tgcaacagct tcttaaaatc aacttgatat gttttgcaac
       61 atatgtgacc tgacagccaa atccaagtaa caggaattta atcatgttta cagtaatgta
      121 accttcccgt aaaatgccca cacactttaa acgccaccag atcccgtgga attgaggtcg
      181 ttaaatgaga ctcaggaaat acaataaaag tttgggatgg ttgtcattat ttgcaggcac
      241 tgtattgctc agtggctgta attctgcgct gttagatccc aaaggacaga ttggtctgga
      301 gcaacgttca ctgatactga cggcatttgg cctgatgttg attgtcgtta ttcccgcaat
      361 cttgatggct gttggtttcg cctggaagta ccgtgcgagc aataaagatg ctaagtacag
      421 cccgaactgg tcacactcca ataaagtgga agctgtggtc tggacggtac ctatcttaat
      481 catcatcttc cttgcggtac tgacctggaa aaccactcac gctcttgagc ctagcaagcc
      541 gctggcacac gacgagaagc ccattaccat cgaagtggtt tccatggact ggaaatggtt
      601 cttcatctac ccggaacagg gcattgcaac cgtgaatgaa atcgctttcc cggcgaacac
      661 tccggtgtac ttcaaagtga cctccaactc cgtgatgaac tccttcttca ttccgcgtct
      721 gggtagccag atttatgcca tggccggtat gcagactcgc ctgcatctga tcgccaacga
      781 acccggtact tatgacggta tctccgccag ctacagcggc ccgggcttct caggcatgaa
      841 gttcaaagct attgcaacac cggatcgcgc cgcattcgac cagtgggtcg caaaagcgaa
      901 acagtcgccg aacagcatgt ctgacatggc agcgttcgaa aaactggccg cgcctagcga
      961 atacaaccag gtggaatatt tctccaacgt gaaaccagac ttgtttgccg atgtgattaa
     1021 caagtttatg gctcacggta agagcatgga catgacccag ccagaaggtg agcacagcgc
     1081 acatgaaggt atggaaggca tggacatgag ccacgcggaa tccgcccatt aaaggggttg
     1141 aggaagaata aagatgttcg gaaaattatc acttgatgca gtcccgttcc atgaacctat
     1201 cgtcatggtt acgatcgctg gcattatttt gggaggtctg gcgctcgttg gcctgatcac
     1261 ttacttcggt aagtggacct acctgtggaa agagtggctg acctccgtcg accataaacg
     1321 cctcggtatc atgtatatca tcgtggcgat tgtgatgttg ctgcgtggtt ttgctgacgc
     1381 cattatgatg cgtagccagc aggcccttgc ctcggcgggc gaagcgggct tcctgccacc
     1441 tcaccactac gatcagatct tcaccgcgca cggcgtgatt atgatcttct tcgtggcgat
     1501 gcctttcgtt atcggtctga tgaacctggt ggttccgctg cagatcggcg cgcgtgacgt
     1561 tgcgttcccg ttcctcaaca acttaagctt ctggtttacc gttgttggtg tgattctggt
     1621 taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc tggctggcct atccaccgct
     1681 atcgggaata gagtacagtc cgggagtcgg tgtcgattac tggatatgga gtctccagct
     1741 atccggtata ggtacgacgc ttaccggtat caacttcttc gttaccattc tgaagatgcg
     1801 cgcaccgggc atgaccatgt tcaagatgcc agtatttacc tgggcatcac tgtgcgcgaa
     1861 cgtactgatt attgcttcct tcccaattct gacggttacc gtcgcgttgt tgaccctgga
     1921 tcgctatctg ggcacccatt tctttaccaa cgatatgggt ggcaacatga tgatgtatat
     1981 caacctgatt tgggcctggg gccacccgga agtttacatc ttgatcctgc ctgtattcgg
     2041 tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt ctgtttggtt atacctcgct
     2101 ggtatgggca accgtctgta tcaccgtgct gtcgttcatc gtctggctgc accacttctt
     2161 tacgatgggt gcgggcgcga acgtaaacgc cttctttggt atcaccacga tgattatcgc
     2221 catcccgacc ggggtgaaga tcttcaactg gctgttcacc atgtatcagg gccgcatcgt
     2281 gttccattct gcgatgctgt ggaccatcgg ttttatcgtc accttctcgg tgggcgggat
     2341 gactggcgtg ctgctggcag taccgggcgc ggacttcgtt ctgcataaca gcctgttcct
     2401 gattgcgcac ttccataacg tgatcatcgg cggcgtggtc ttcggctgct tcgcagggat
     2461 gacctactgg tggcctaaag cgttcggttt caaactgaac gaaacctggg gtaaacgcgc
     2521 gttctggttc tggatcatcg gcttcttcgt tgcctttatg ccactgtatg cgctgggctt
     2581 catgggcatg acccgtcgtt tgagccagca gattgacccg cagttccaca ccatgctgat
     2641 gattgcagcc agcggtgcgg tactgattgc gctgggtatt ctctgcctcg ttattcagat
     2701 gtacgtttct attcgtgacc gcgaccagaa ccgtgacctg actggcgacc cgtggggtgg
     2761 ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc tataactttg ccgttgtgcc
     2821 gcacgttcac gagcgtgatg cattctggga aatgaaagag aaaggcgaag cgtacaaaaa
     2881 gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc ggcgccggta tcgtcattgc
     2941 ggctttctcc accatcttcg gtttcgccat gatctggcat atctggtggc tggcgattgt
     3001 tggcttcgca ggcatgatca tcacctggat cgtgaaaagc ttcgacgagg acgtggatta
     3061 ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag catttcgatg agattactaa
     3121 ggcagggctg aaaaatggca actgatactt tgacgcacgc gactgcccac gcgcacgaac
     3181 acgggcacca cgatgcaggc ggaaccaaag tcttcggatt ctggatctac ctgatgagcg
     3241 actgcattct gttctctatc ctgtttgcta cctatgccgt tctggtgaac ggcaccgcag
     3301 gcggcccgac aggtaaggac attttcgaac tgccgttcgt tctggttgaa actttcctgc
     3361 tgttgttcag ctccatcacc tacggcatgg cggctatcgc catgtacaaa aacaacaaaa
     3421 gccaggttgt ctcctggctg gcgttgacct ggttgtttgg cgccggattt atcgggatgg
     3481 agctctatga attccatcat ctgattgtta acggcatggg tccggatcgc agcggcttcc
     3541 tgtcagcgtt ctttgcgctg gtcggcacgc acggtctgca cgtcacttcc ggtcttatct
     3601 ggatggcggt gctgatggtg caaatcgccc gtcgcggcct gaccagcact aatcgtaccc
     3661 gcatcatgtg cctgagcctg ttctggcact tcctggatgt ggtttggatc tgtgtattca
     3721 ctgttgttta tctgatgggg gcgatgtaat gagtcattct aacgtgagcg gcggcgcatc
     3781 ccatggcagc gtaaagacct acatgacagg ctttatcctg tcgataatcc tgacggtaat
     3841 tccgttctgg atggtgatga caggggctgc ctctccggcc gtaattctgg gaacaatcct
     3901 ggcaatggca gtggtacaga ttctggtgca tctggtgtgc ttcctgcaca tgaataccaa
     3961 atcagatgaa ggctggaata tgacggcatt tgtcttcacc gtgctaatca tcgccatcct
     4021 ggttgtgggc tccatttgga ttatgtggaa cctcaactac aacatgatga tgcactaaga
     4081 gcggcggtta tgatatttaa gcaatacctg caagtaacga aaccaggcat catctttggc
     4141 aacctgatct cggtgattgg gggattcttg ctggcctcaa agggcagcat tgattatccc
     4201 ctgtttatct acacgctggt tggggtgtca ctggttgtgg cgtcgggttg tgtgtttaac
     4261 aactacatcg acagggatat cgacagaaag atggaaagga cgaagaatcg ggtgctggtt
     4321 aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca ccttgctggg tattgctggc
     4381 tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct ggctgggggt gatgggcttt
     4441 gtggtttatg tcggcgttta tagcctgtac atgaaacgtc attctgtcta cggcacattg
     4501 attggttcgc tctccggcgc tgcgcctccg gtgatcggtt actgtgcggt aaccggcgag
     4561 ttcgatagcg gcgcagcgat cctgctggct atcttcagcc tgtggcagat gcctcactct
     4621 tatgccatag ccattttccg ctttaaggat tatcaggcgg caaacattcc ggtactgccg
     4681 gtggtgaaag gcatttcggt ggcgaagaat cacatcacgc tgtatatcat cgcctttgcc
     4741 gttgccacgc tgatgctctc tcttggcggc tacgctgggt ataaatatct ggtggtcgcc
     4801 gctgcggtga gcgtctggtg gttaggcatg gctctgcgcg gctataaagt tgctgatgac
     4861 agaatctggg cgcgcaagct gttcggtttc tctatcatcg ccatcactgc cctctcggtg
     4921 atgatgtccg ttgattttat ggtgccggac tcgcatacgc tgctggctgc tgtgtggtaa
     4981 cacaacctct cagttaaaaa ggtgctacgg cacctttttt cttagcatta gaatcatatc
     5041 cctctcgaaa tatttactaa aaaatccgca tgtttacccc attcgtttgc cgctttacac
     5101 tagtcgcgaa tttaaaacag aggtggtaat gaacgattat aaaatgacgc caggtgagcg
     5161 gcgcgcgacc tggggtttag ggaccgtatt ttcattacgc atgctgggca tgttcatggt
     5221 tctgccggtt ctgaccacgt acggcatggc tctgcaaggt gccagcgaag cattaatcgg
     5281 tattgccatt ggtatttatg gtctgactca ggccgttttt cagattccgt ttggcctgct
     5341 ttcagaccgc attggtcgca aaccattaat tgtcggtggg ctggcggtgt ttgccgccgg
     5401 tagcgttatc gctgcgctct ctgactccat ctggggaatt attctgggtc gggcgctaca
     5461 aggctccggt gcgattgccg ctgccgttat ggcgctgctt tccgatctca cgcgcgaaca
     5521 aaaccgcacc aaagcgatgg cgtttatcgg cgtgagcttt ggcattacct ttgccattgc
     5581 gatggtgctt ggcccgatca tcactcacaa acttgggctg cacgcgctgt tctggatgat
     5641 cgctattctg gcaacaaccg gcattgcgtt gaccatttgg gttgtgccca acagtagcac
     5701 tcacgtactt aatcgtgagt ccggaatggt gaaaggcagt ttcagtaaag tgctggcgga
     5761 accgcggctg ctgaaactca actttggcat tatgtgtctg catattttgc tgatgtcgac
     5821 ctttgttgcc ctgcccggac agatggctaa tgcggggttc ccggcggctg aacactggaa
     5881 ggtctacctg gcaacgatgt taatcgcctt tggctcggtc gtgcctttca ttatctacgc
     5941 tgaagttaag cgaaaaatga agcaagtctt tgtcttttgc gtcgggttga tcgtggtcgc
     6001 ggaaattgtg ttgtggaacg cacaaacgca gttctggcaa ctggtggtcg gcgtgcagct
     6061 tttctttgtg gcgtttaatt tgatggaagc ccttctgcct tcacttatca gtaaagagtc
     6121 gccagcaggt tacaaaggta cggcgatggg tgtttactcc accagccagt ttcttggcgt
     6181 ggcgattggc ggttcactgg gcggctggat tgacggcatg tttgacggtc agggcgtatt
     6241 tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc gccagtacca tgaaagaacc
     6301 gccgtatgtc agcagcttgc gcattgaaat cccggcgaac attgccgcaa acgaggcgtt
     6361 aaaagtgcgt ttgctggaaa ctgaaggcat caaagaagtg ttgattgcag aagaagaaca
     6421 ttcagcttat gtgaaaatcg acagcaaagt gacgaatcgc tttgaggttg aacaggcaat
     6481 tcgccaggca taaaagcgag gcgaaattgc ctgctgcgct acgcttatca ggcctactaa
     6541 gttcctgagt gacaaacctt tgtaggccga atcaggcgtt catgccgcat ctgacatgaa
     6601 caaagcgcag gttgtcgcga ttaatcgcgg aagtttttga actggaacgg ctgaccgaga
     6661 tcgccaccac gtaccatcgc catgacagcc tgtaaatcat cacgagattt gcccgtcaca
     6721 cggatctcat cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcattttg
     6781 acgattttct tctgagtcgc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag
     6841 gttttaccgc tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc
     6901 ttcagcaact tggcacgcag aatatccagc aactgattga cctggaaatc ggactcgctc
     6961 aacactttga tggttttgct ggcgtcgttc agctcaaatg aggcttcaac attacggaag
     7021 tcaaaacggg actccacttc gcggctcgcg ttatcgaccg cgttacgtgc ttcctgaaga
     7081 tcaacttcag agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg
     7141 aagcataata cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat
     7201 tgtgcatagt cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg
     7261 ttgcgggtga ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt
     7321 atggcttaca gcactttgta aacagggtca cgacgttcag ggctggctgc gcgtaccgca
     7381 accttattgt agcgtgaatc tggttgagac agatggttcg atatttaacg agtctctgac
     7441 cgccaacgat cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg
     7501 gcaggtttcc gatgccgtca aaagcctcgc gtccacactg cctgtaacca cgccaatact
     7561 gttaattcac aacggcatgg gcaccatcga agagttgcaa aacattcagc agccattact
     7621 gatgggcacc accacccatg ccgctcgccg cgacggcaat gtcattattc atgtggcaaa
     7681 cggtatcacg catattggtc cggcacggca acaggacggc gattacagtt atctggcgga
     7741 tattttgcaa accgtattgc ctgacgtcgc gtggcataac aatattcgcg ccgagctgtg
     7801 gcgcaagctg gcagtcaact gtgtgattaa tccactgacc gccacctgga attgcccgaa
     7861 tggtgaatta cgtcatcatc cgcaagaaat tatgcagata tgcgaagaag tcgcggcagt
     7921 gatcgaacgc gaaggacatc atacttcagc agaagatttg cgtgattacg tgatgcaggt
     7981 gattgatgcc acagcggaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca
     8041 caccgaaatc gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc
     8101 cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg
     8161 catcggcact ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accactatcg
     8221 acctcttggt tcgcggcggt atcaaagtca ccactgccag cgtcgccagc gatggtaacc
     8281 tggcgattac ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gttgaagtgg
     8341 ctgatggcga atatgacgtg attgtgctgc ctggtggcat taaaggcgca gagtgttttc
     8401 gcgatagcac cctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg
     8461 cggctatttg cgccgcacca gccaccgtgc tggtcccgca cgatatcttc ccgattggca
     8521 atatgaccgg cttcccgacg ctgaaagaca aaatacccgc cgaacaatgg caggacaagc
     8581 gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca ggggccaggt acagctatcg
     8641 actttggtct gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat
     8701 cacaactggt gatggcggca gggatttata attattacga gtagtgtcgg atgcggtaaa
     8761 cgtcgcatct gaccagatgc gacgttacaa ccacatatta cgggcgatac accttcacat
     8821 tgttaaagcc ctgctcgcgc agatagagcg cctgcaggcg gctcatcacc ccgcgctcac
     8881 accacaacag ccaggttttg ctctggtcga gatcgccaaa tttggtgctc agtttataga
     8941 acggcagaga aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga
     9001 tagaacggat atcgaggatc acgtcgttcg ggccgaagcc attgacggtt tccacttcca
     9061 ccacttcctg ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcca
     9121 ccactttatc gagaatgctg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa
     9181 ccgctttcac cgtcgggctt ttggaaatca cgccgcaata ttccggcatc gtgcgggcaa
     9241 agtcttcggt gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagagatca
     9301 gcggacgcag gatcagcgta tcggagacgt tatcaatcag gcgtaggttg gtcagcgtct
     9361 ggctggacac ctggccgagc gcttcaccgg taaccagcgc ctgtacgccg taacgttctg
     9421 ccactttgga cgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt
     9481 cgattttctc gagaatttcc ccgacgaccg gttcgaaatt aatagcgaca aaacgtacgc
     9541 ggtgggagct gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat
     9601 gcgccgcgcc gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca
     9661 tataactgga aacgccggag tcgaaaccac ctgaaatgag cgacaacaca tcttcctggg
     9721 tgccgatcgg gaaaccgcca ataccttcgt agcggccttt aatcagcagc agacgatcgt
     9781 cttccacttc cagatggaca gtcacttccg gattggtcag cttcacgcgc gcggattcaa
     9841 tatgctgatt taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt
     9901 tgccgcggcg cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatattgaa
     9961 ccaacgcttt ctcgaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa
    10021 tatggtggat acccggaata cgggtcaggg cgtcgcgaat agccagacgc tggttttcat
    10081 cttttgcgcg aacttcgatg ttatcccagt gacggacgac agcgagcgtc tcatcatagt
    10141 gctttaaaac gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt
    10201 ggcttttgat ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc
    10261 gttggcaagc cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc
    10321 atccaggcgc ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc
    10381 gcgttatttg ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc
    10441 agacattcat tatgccgaag aaaaatgagg ctcccgccag ctttgaaaag gcgctgagcg
    10501 agctggaaca gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga
    10561 acgagttcga acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg
    10621 aacagcgcgt acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac
    10681 cggacaatga gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca
    10741 ggcgctgagc cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat
    10801 gcagtatggc gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccactgg
    10861 tcatatgttc ggcgttagca caaacacact ggacgcaccc gctgccgccg tagagtgtat
    10921 ccacgcttac tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg
    10981 cggtttgccg acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggtgacgc
    11041 tttacaaacg ctggcgttct cgattctaag cgatgccgat atgccggaag tgtcagatcg
    11101 cgacagaatt tcgatgattt ctgagctggc gagcgccagc ggtattgccg gaatgtgcgg
    11161 tggtcaggca ttagatttag acgctgaagg caaacacgta cctctggacg cgcttgagcg
    11221 tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag
    11281 cgccggagat aaaggacgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg
    11341 ccttgccttc caggttcagg atgacatcct ggatgtggtg ggagatactg caacgttggg
    11401 aaaacgccag ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct
    11461 tgagcaagcc cggaagaaag cccgggatct gatcgacgat gcccgtcagt cgctgaaaca
    11521 actgactgaa cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca
    11581 gcgtaataaa taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata
    11641 cccgaccctg gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt
    11701 accgaaactc tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg
    11761 gcacttcgcc tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa
    11821 taccccgttt gaccagttaa tctgggatgt ggggcatcag gcttatccgc ataaaatttt
    11881 gaccgggcgc cgcgacaaaa tcggcaccat ccgtcagaaa ggtggcctgc acccgttccc
    11941 gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag
    12001 tgccggaatt gggatcgcag ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg
    12061 tgtcattggc gatggcgcga ttactgctgg catggcgttt gaagcgatga atcacgcggg
    12121 cgatatccgt cctgatatgc tggtggttct caacgacaat gaaatgtcga tttccgaaaa
    12181 tgttggcgcg ctcaacaacc atctggcgca gctgctttcc ggtaagcttt actcttcact
    12241 gcgcgaaggc ggaaaaaaag ttttctctgg cgtgccaccc attaaagagc tgctcaaacg
    12301 taccgaagaa catattaaag gcatggtagt gcctggcacg ttgtttgaag agctgggctt
    12361 taactacatc ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgcttaagaa
    12421 catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta
    12481 tgaaccggca gaaaaagacc caatcacctt ccacgccgtg cctaaatttg atccctccag
    12541 cggttgtctg ccgaaaagta gcggcggttt gccaagctat tcaaaaatct ttggcgactg
    12601 gttgtgcgaa accgcagcga aagataacaa gctgatggcg attactccgg cgatgcgtga
    12661 aggttccggc atggtcgagt tttcacgtaa attcccggat cgctacttcg acgtggcaat
    12721 tgccgagcaa cacgcagtga cctttgctgc gggtctggcg attggaggct acaaacccat
    12781 tgttgcgatc tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc
    12841 gattcaaaag cttccggtcc tgttcgccat cgaccgtgcg ggcattgttg gtgctgacgg
    12901 tcaaacccac cagggcgctt ttgatctctc ttacctgcgc tgcataccgg aaatggtcat
    12961 tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa
    13021 cgatggcccg tccgcggtgc gctacccgcg cggcaacgca gttggcgtgg aactgacgcc
    13081 actggaaaaa ctgccaattg gcaaaggcat tgtgaagcgt cgtggtgaga aactggcgat
    13141 ccttaacttt ggtacgctga tgccagaagc ggcgaaagtc gctgaatcgc tgaacgctac
    13201 gctggtcgat atgcgtttcg tgaaaccgct tgatgaagcg ttaattctgg aaatggccgc
    13261 cagccatgaa gcgctggtta ccgtagaaga aaacgccatt atgggtggtg caggcagcgg
    13321 cgtgaacgaa gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggtctgcc
    13381 ggacttcttt attccacaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc
    13441 caccggtatg gaagccaaaa tcaaagcctg gctggcataa tccctactct actcctgcta
    13501 tgcttaagaa attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac
    13561 cgaccttcgt gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg
    13621 taatcacgca tggacactgc cggaagagag cagccgtccc atcattaaac gcgcactgga
    13681 aggcggcata aatttctttg attccgccaa tagctattcc gatggcagca gcgaagagat
    13741 cgtcggtcgc gcactgcggg atttcgcccg tcgtgaagac gtggtcgttg cgaccaaagt
    13801 gttccatcgc gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat
    13861 cgacgacagc ctgctccgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg
    13921 ggattacaac acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg
    13981 gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcactgga
    14041 actacaaaaa cagcacggct gggcgcagtt tgtcagtatg caggatcact acaatctgat
    14101 ttatcgtgaa gaagagcgcg agatgctgcc gctgtgttat caggaaggcg tggcggtgat
    14161 tccatggagt ccgctggcgc ggggccgtct gacgcgtcct tggggagaaa ccaccgcacg
    14221 gctggtgtcg gatgaagtgg ggaaaaatct ctaccaagaa agcaatgaaa atgacgcaca
    14281 aattgcagaa cgcttaacgg gcgtcagtga agaacttggt gcaacgcgag cacaagttgc
    14341 gctggcctgg ttgttgagta aaccgggcat tgccgcaccg attatcggaa cttctcggga
    14401 ggaacagctt gatgaactgc taaacgcggt ggatatcacc ttgaagccgg aacagatcgc
    14461 cgagctggaa acgccgtata aaccgcatcc ggtcgtagga tttaaatagg tatgcaggcc
    14521 tgatgagacg tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag
    14581 cggccagtga tgaccgataa aatacaggat gccagcggag atcaccccgg cgacaatatc
    14641 gtcaatcatg atccccatgc cgccatgtac attgtgatca aaccagcgga tcggccacgg
    14701 cttccacata tccagaatac ggaaaatcac aaatccggcg gtaacccact gccagtcatt
    14761 ggtcggcagc gccatgagcg taatccacat accaataaat tcgtcccaga caatactgcc
    14821 gtgatcgtgc acgcccatgt ctttcgccgt ttgatgacaa agatagacgc cgatacagat
    14881 ccccagcatt accaccagcg aatagagctg ccagggcaaa aaagtcatca gataccagaa
    14941 gggaatcgcc gccagcgacc ccatcgtgcc aggaacgatc gggcttaatc cacttccgaa
    15001 tccgacagca agcagatgcc acggattact cattttcagg cgacttttcg cgacatcttt
    15061 atggcgtggc aaaatggtca tatcctttcc agtctaacgt gacaggttcg ccgtcacgaa
    15121 taaaacaaag cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccca
    15181 ggtgtccgag agccacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact
    15241 cgtaatcttc accaccagag agcgcccaac gcagcgcctg ttccggttca acatggcgag
    15301 aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt
    15361 tcacgatatg cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca
    15421 gatcgcgcag tgcctgcccc tgtaaaatac gcggcgacgg acggagatga cgtttgatca
    15481 agtagtccgc atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg
    15541 cagcgctatc gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc
    15601 gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc
    15661 cacgcgtggt atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaagc
    15721 tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca
    15781 gccaggccgg atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat
    15841 aagccagatc agcaggatcg atatcaggga ggaagtggtt gcccgccacc agcgtatcag
    15901 tgctgatcgc cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc
    15961 ccagttcgac atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga
    16021 actcgccaca tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg
    16081 gaacggtctt ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg
    16141 tgctgcttta tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt
    16201 cgccagttcg atcgcttcgt taatggccac tttgtatggc acatcgctac gtttagacag
    16261 ctcgtacagc gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcagacg
    16321 ggacaggtac ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag
    16381 cagctcacgg aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta
    16441 ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca
    16501 ctcacgagcg cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct
    16561 tgatggcttt caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt
    16621 tgccagcttt ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc
    16681 caaaagcaac cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac
    16741 cagcgacata ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg
    16801 cgtcgtattt accggtttta gccagtgcac ccgccgccag cggcagctca taggcacctg
    16861 gcacccaaac aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa
    16921 ttgcaccttc cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga
    16981 cgcgagcgtc cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc
    17041 gagtatggcc ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc
    17101 gagccgggag atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc
    17161 tctttgaatt taaattgggg agcgtcggct aatttctcaa gccctggcag cgtgcataat
    17221 ccacgggcgt cgttgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa
    17281 cccgcctgta gcaatgcgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc
    17341 tgctgtttac ccagttgcat cattagtaca accagatcca ggtgaccttt atgctctgga
    17401 atcagcaagg tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac
    17461 caggtttcgc cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca
    17521 atcacaatac gtaccggctg gcggagattt tgttgcgggt agagtccctg agcttgttca
    17581 tccagttcag accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg
    17641 gttaaaatgg catgactttg cgcgcgcagt cgttgtacgt cgcgcctcgc ctgaggtgaa
    17701 gtgatccact ggctttcgcc actcgccatc gccgtgcgac catcaagcga tgcgccaagt
    17761 tttaactgaa tataaggaaa gccggtgcgc atccgtttga gaaagccttt attcaattgc
    17821 tcagcttcac tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa
    17881 agtccacgtc cagcgacctg cggattgggg tcttgcattg cagcaaccac gcgcgccacg
    17941 cccgcagcaa ttaatgcatc acaacacggt ggcgtacgac catgatggct acagggttcg
    18001 agtgtgacat acgccgttgc gcctttggct ttttcgccgg ccatgcgcaa cgcgtgtacc
    18061 tcggcatgcg gttcacccgc gcgttgatgg taaccttcac cgacaatttc gccatctttg
    18121 acaatgacgc acccgacatt cgggttggga tgcgtggtaa aacgtcctcg ttgcgccagc
    18181 tttagcgccc gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc
    18241 tcttcgccaa attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag
    18301 gcgactttat cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggc
    18361 acttcgcgct caccggtggc gcgcagctgc gattttatat gattgattgc catttcgacg
    18421 tcatcggaac tcaccggacg tttttccagc gcccgcagca ttccgctacg taatttctct
    18481 tcattaaacg gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc
    18541 acttcaaagg tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat
    18601 gaaccctcgc ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg
    18661 caatgcatac ggcgtcctga ccaggtggtt aacagaaatc tattttaccc tgaactgacg
    18721 atacaacaaa ggcgcaacgc ttttgagata aaggatcgat gctttcactg cttttaccct
    18781 ctaccattag gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac
    18841 tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac
    18901 agatgaaaca cagcgtcagc acgctgaacc aggagatgac gcaactcaat aaagaaacgg
    18961 tcaaaatcac tcagcaaaac aagctgaatg caaaatccag cagtggggtt taccttctgc
    19021 ctggagcaaa aacaccagca agactggaaa gccagatcgg tactttacgt atgtcgctgg
    19081 tgaatattac gcctgacaca gatggcacta ccctgacgct acgtattcag ggcgaatcca
    19141 atgacccttt acccgcattc agcggaaccg ttgaatatgg gcaaattcag ggaacaatag
    19201 acaactttca ggaaatcaat gtgcagaatc aattgattaa cgcacctgcc agcgtcctcg
    19261 cccccagcga tgttgatatt ccgttacagt taaagggtat gtctgtggat cagttaggct
    19321 ttgtacgtat ccacgacatt caacctgtta tgcagtaaac gtattgcggg gcgatattgt
    19381 gcgtcccgca acatctttct ccgtcatttt gttactccgc ttacatcacc tggattgata
    19441 gtaaaagttt gcaacaaggg tgaaagtcag tacaatcccc gcccgaatgt gtgtaaacgt
    19501 gaacgcaatc gattacgtaa atgatagaac tgtgaaacga aacatatttt tgtgagcaat
    19561 gatttttata ataggctcct ctgtatacga aatatttaga aacgcaattt gcgccttttt
    19621 cactcccgca agggattttc aaacagtggc atacatatga aaaaaacatt actggcagcc
    19681 ggtgcggtac tggcgctctc ttcgtctttt actgtcaacg cagctgaaaa cgacaaaccg
    19741 cagtatcttt ccgactggtg gcaccagagc gttaacgttg tcggaagcta tcacacccgt
    19801 ttcggaccgc agctccgcaa cgatacctac cttgagtacg aagcattcgc taaaaaagac
    19861 tggttcgact tctatggtta tgcggatgcg ccggtattct tcggcggtaa ctccgatgca
    19921 aaaggtatct ggaaccacgg ttctccgctg tttatggaaa tcgaaccgcg tttctccatc
    19981 gacaagctga ccaatactga ccttagcttt ggtccgttca aagagtggta cttcgcgaac
    20041 aactacattt acgacatggg tcgtaataaa gatggccgcc agagcacctg gtacatgggt
    20101 ctgggtaccg acatcgatac tggtctgccg atgagcctgt ccatgaacgt ctatgcgaaa
    20161 taccagtggc agaactacgg cgcagcgaac gaaaacgagt gggacggtta ccgtttcaaa
    20221 gtgaagtact ttgtgccgat taccgatctg tggggcggcc agctgagcta catcggcttc
    20281 accaacttcg actggggttc cgacctgggc gatgacagcg gttatgcgaa caacggtatt
    20341 aaaacacgta ccaataactc tattgcgtcc agccatattc tggcgctgaa ttacgatcac
    20401 tggcactact ctgtcgttgc ccgttactgg cataacggtg gtcagtggaa tgacgatgca
    20461 gaactgaact tcggcaacgg caacttcaac gttcgctcta ccggctgggg tggttatctg
    20521 gtagtaggtt acaacttcta attgtgagaa tgccggattt catatccggc attttttatc
    20581 acttcttctt gttcatcatc gctttcaaat cagcgaatgg gttgtacttc gcttcaccga
    20641 catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc gcttcggtgt
    20701 atttcgaatg ctcatgatcg tggcaataga gacaaagtaa ttcccagtta ctgccatctt
    20761 ccgggttatt agtatggtcg tgatcaatgt ggtgaacggt aagttcacgc aggttggaat
    20821 aaacaaactc gcgggaacag cgaccgcaga cccacggata gatttttaat gctttttcgc
    20881 gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaaa aagttacctg
    20941 gatgaaatgt agggttacgc gcagtttccc aagatgcggg gattttcgca atcaccttac
    21001 atcaaaaaaa atcccgatct tctgaccggg attttcaaca tcagctgaaa cttgattaag
    21061 gcagaattga cggctgatcc gccccttctt tttccacttt ctgctgcaac atgtgttcgc
    21121 gcttcatacc cagtttcaga gccagcgcag atgccacata gatggaagat gcagtaccga
    21181 tggaaacacc gataagcatg gtcagcgaga agccttccag taccggacca ccgaagaggt
    21241 acagcatcag gataaccatc aaggtagtac cggatgtgat caaggtacgg tgcagcgtct
    21301 gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg acggatcttg cggaagtttt
    21361 cacgaatacg gtccgatacc acgatactgt cgttaagcga gtaaccgata accgacatca
    21421 acgatgccac aatggtcagg tcaatctcga tatggaataa cgacaaaata cccagcgtaa
    21481 tgataacgtc gtgcgccagc gcaataacaa cccctgccgc cagtcgccac tcaaagcgga
    21541 aacctacgta cacgaggata gacagcagcg ccgccatcaa cgccatcgca ccggtttgcg
    21601 caaggtctgc ccccacgctc ggaccgacga actcaatacg cttcactgcc gcattctgat
    21661 tggtggattc gttaatcacc ttcagaacct ggctgcccag cacctgaccg ccggtttcgc
    21721 cttcagcagg cggcatacgg accatgatgt cgtggctgct accaaagttt tgcagcatcg
    21781 gctcttcaaa accggctttt tgcaatgcat cacgcattac gtcaatttca gccggttttt
    21841 cgagcgtaat ttcaataacc gtaccaccgg taaaatccag cccccagtta aagccgcgca
    21901 cgcccataat aacgatagcg gcgattaaca gcagaccaga gatgccgaaa gcccagtagt
    21961 cccagcgcat aaagtcatag actttacggc cgtggtttag ttgttcaaca gtatattcct
    22021 gtgccacatc gcactcctca gattgacagc ttcttgacgc gcttgccgcc atatagcagg
    22081 tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg acgtcgccac accgataccg
    22141 gtagtaatcg cgaacccttt aattgccccg gtacccactg catacaggat gatgacttta
    22201 atcagcgtgg tgatgttcgc atcgaagatg gaactgaatg cgccacgata accttcatca
    22261 attgcctgtt gaacagtacg tccgttgctc aactcttctt taatacgttc gttgatcagt
    22321 acgttcgcat cgaccgctac cgcaagggtt aagacgatac ccgcgatacc tggcatactc
    22381 agcgttgcgc ctggcaacag cgacataata ccgacaatca agatcaagtt ggcaatcaga
    22441 gcactggtcg caatcagacc aaacttctta tagaagatga tcatgaacag aatagaaacc
    22501 agcagaccgg caaggcaagc ctccagcccc tgctcaatgt tctgcatacc cagggttggg
    22561 ccaatggtac gttcttcaac aatctgaatc ggcgcgatca acgcaccggc acgcagcagc
    22621 agtgaaagct gacgcgcttc gcttgggtta ttgatgccag taatacggaa actgttaccc
    22681 agacgagact ggatgttggc gatgttaatc acctcttcct gtttcaccag aaccgcacga
    22741 ccgtttgcat ctttcttacc gctgtctttg tactccacaa acagagttgc catcggtttg
    22801 ccgatgttgt ccttggtgaa gttagacatg atgttaccac ccgcgctatc gagcgagatg
    22861 ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg agtcggtgat atggtcaccg
    22921 gtcagaatta cgcgtttgta cagcacaacc ggctgacctt cgcgggtctg tttcacttca
    22981 gagtcgcccg gtacgcgacc ggatgccgca gcggcctgat caacgttggt gtttaccaga
    23041 cggaattcca gcgttgccgt cgcacccaga atctctttcg cacgcgcagt gtcctgaata
    23101 cctggcagtt caacaacgat acggtcagca ccctgacgct gaaccaccgg ctccgccacg
    23161 ccaagttggt ttacacggtt acgcaggata ttgatgttct gctgcaccgc atattcacgc
    23221 gcttcactca gacgagcatc gctcattacc gcacgcagct ggttgctgcc ctggctgcta
    23281 atcaccaggt ccggatggcg cttgctcaga tacgcaatgg cttcatcacg agctttagca
    23341 tcgcggaaag tgatgctcag gccgtagttg ttttctttac gaacggtggt atacgggatg
    23401 cccttttcac gcaggtcgct gcgcaggcta tcgatatttt gttcctggag tttgccaagc
    23461 gcggtgtcca tatccacttc catcaggaag tgaacgccgc cacgcaggtc aaggccgagc
    23521 ttcatcggct cagcatgaat ggctgccagc cagcgcggcg ttgccggggc aagattaagc
    23581 gccacgacgt atttgtcacc cataacgccc attaatgctt cacgagcgcg cagctgggtg
    23641 tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca gtgccacaga cttagcagtt
    23701 attttttctt cttgtaacgt tttctggacc tggatcagcg tttgctcact ggcggcgact
    23761 ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa acaggttggg aagcgcatac
    23821 agcagaccga tgacaatcac cacgatcagc atgacgtact tccacaaagg ataacggttt
    23881 aacacggcaa ttcccttagg gaaaaatttt aattacagcg ccttcatggt gcctttcggc
    23941 agaacggcag ctacgaagtc acgtttaata actacttcag tggtgtcatt cagtgcgata
    24001 gcaatgtagc cgttttccgc tactttggtt acgcgaccga ccaggccacc gttcgtcagc
    24061 acttcatcac ctttagcgat ggagtccatc agctttttgt gttctttggt gcgcttctgc
    24121 tgtggacgca ggatcatgaa atagaaaatc agaccgaata ccaccagcat caaaatcaaa
    24181 gacatcgggc taccttgcgc cggtgcaccc gttgccgcta ccgcatcaga aataaaaaag
    24241 ctcattaaat ttccctcatt attaatatta atcaacgttc aaaggtggta cttctcgccc
    24301 ctgacgctgg taaaaatcag ttacgaagct ctctaattta ccctcttcaa tagccttgcg
    24361 taaacccgcc atcaaacgct ggtagtaacg aaggttatga atggtgttga gtcgcgcgcc
    24421 taatatttcg ttgcaacggt cgagatgatg caagtaagcg cgtgaataat tgcgacaggt
    24481 gtagcaatca cactcaggat cgagtgggcc agtatcgctc ttatacttcg cattgcggat
    24541 tttcaccacg ccatcggtca cgaacaaatg accatttcgg gcgttgcggg ttggcattac
    24601 gcagtcaaac atatcgatac cgcgacgtac gccctcaacc aggtcttctg gtttaccaac
    24661 gcccatcagg taacgcggtt tgtctgccgg aatttgcggg catacatgct ccagaatgcg
    24721 gtgcatatct gctttcggct cacccacagc cagaccgccg acagcgtagc catcaaaacc
    24781 gatatctacc agacctttaa cagaaatatc acgtaaatct tcgtaaacac tgccctgaat
    24841 gataccaaac agcgcatttt tgtttccgag actgtcaaaa cgctcacggc tacgcttcgc
    24901 ccaacgcaga gacatctcca tggagcgttt tgcgtaatcc cagtcagcag gatacggcgt
    24961 acactcatca aagatcatga cgatatccga accaagatcg tactgaatct ccatcgactt
    25021 ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggtta cggaagtgaa cgccctgttc
    25081 ggtgatttta cgaatatcac caaggctgaa gacctggaag ccgccggagt cggtaaggat
    25141 tggtcctttc cactgcataa aatcgtgcag atcgccgtgc agtttcatga tttcctggcc
    25201 cgggcgcagc cacaggtgga aggtgttgcc gaggataatt tgcgcgccag tggcttcaac
    25261 ttcttccggc gtcatccctt ttacggtgcc gtaagtgcca acaggcataa aacaaggcgt
    25321 ttccactacg ccacgatcaa agaccaggcg gccacggcgt gcgcgaccgt cggtggtgtc
    25381 cagttcaaat ttcatttttt ctccactacg tcagaaaaac agtccaacgt ttaaaccagc
    25441 gccgcggaat tactccccaa cgcgctcatt aattgcctgc ggattgtacg tgataaacat
    25501 cgcatcaccg taactaaaaa agcgatattt ctcttctacc gccgctttat aggcattcat
    25561 ggtgtgttga taaccagcaa atgccgaaac cagcataatc agcgtcgatt ccggaaggtg
    25621 gaagttcgtc accagcgcat cgaccacttt gtactggaag cccggataga taaagatttg
    25681 ggtatcgtca aagaacggtt caatgagatc gtttttcgct gcctgagccg cactttccag
    25741 cgaacgtact gaggtggtac caaccgcaat cacccggtta ccgcgcgctt tcgccgccag
    25801 taccgcgtct accacatcct gcggcacttc agcgtattcc gagtgcatga tgtgatcttc
    25861 aatggtgtcg acgcgcaccg gctggaaggt gcccgcacca acgtgcaacg tcacaaacgc
    25921 catctccacg cctttggcgc gtaatttttc cagcaaaggt tcgtcaaagt gcagtccggc
    25981 ggtcggtgct gctaccgcgc cgggtttttc gctataaacc gtttgataaa gttcgcggtc
    26041 tgcatcttcg tccggacggt cgatgtacgg cggcagcggc atatggccga tgctgttgag
    26101 aatatccagc accgaacgtt gatcattaaa ttcgacttca aacagtgcgc cgtggcgcgc
    26161 ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc agttctgcgc caggttttgg
    26221 cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg tcgagcatcc gctcaaccag
    26281 cacttcaatc ttgccgccgc tggctttacg cccaaacaga cgcgccggga tcacgcgggt
    26341 attattaaaa accagaagat cgccggggtt gagcttatcg agtaaatcgg tgaaagtacc
    26401 gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa cgacagctac tgcgttcagg
    26461 catgggatag tgggcaatca gggattcggg caattcaaaa gagaaatcgg taacgcgcat
    26521 gacactgact cagactaaaa taagaggcgg gtagtctagt gccggggcgc tctccctgca
    26581 acctttaccc ccctttggat agaatataat cgatgaattt tttagctcac ctgcatttag
    26641 cccatctcgc ggaaagctcg ctttccggca atttactggc tgatttcgta cgcggaaatc
    26701 ctgaagaaag ttttccgccc gacgtcgtgg ctggcattca tatgcatcgg cgaatcgacg
    26761 tattaaccga caatctgcca gaagtccgcg aagcgcggga gtggtttcgt aatgaaacgc
    26821 gccgtgtcgc gcctattacg ctggatgtga tgtgggatca ctttctgtcc cgccactggt
    26881 cgcagctgtc gcccgatttt ccgctacagg aatttacctg ttatgcccgg gagcaagtga
    26941 tgactattct tccagactca ccgccacgct ttatcaatct gaacaactac ttatggtcag
    27001 agcggtggtt ggtgcgctat cgcgatatgg atttcatcca gagcgtgtta aacggcatgg
    27061 caagccgtcg cccacgtctg gatgccctgc gtgactcctg gtacgattta gacgcccatt
    27121 acgatgctct cgaaacccgc ttctggcagt tttatccgcg gatgatggag caggcgtcac
    27181 gcaaggcgtt ataagacgtt aattcatcca caccgtagcc gaaatggcag gcaaagccag
    27241 aatcccgtcc gtcagttgcc cgtgaccttc tttgcgttgc cattgcgcga cattaagcaa
    27301 cggcgacgcg ggtagcacca cttcacaggc ttcgccacgg ttgattgcca ccagtacacg
    27361 ctgctgattc agcacgcgga caaataccac cacgttatct tccgcataca gcacctgaca
    27421 gccgccacga cgcaacgcct gacttttctt acgcagcgca atcattcgct ggtacagcgc
    27481 gaataacgcc gaatcctgct tttccacctg ccaggggaac ggcttacggc aaaacggatc
    27541 gtttttgcca tccagtccca cttcatcacc gtaataaatg cacggtacac caggccaggt
    27601 gaacagccag accaccgcca ggggcaggcg cgcaacatcc cgaccgagca gcgttttaaa
    27661 tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt aattgttgtt gatgagaaag
    27721 ccctgcgcgg taattatcca tccaggccat acaggtttgg gcgtcaattt gctgcggatc
    27781 gtaagagata tcggtgttgg caagaaatcc ccacaacggg aatgtgaagc cgcgatagtt
    27841 catggcggca tcttccacat cggcctgtaa ccattgccgt gcatcgccaa aatgttcgcc
    27901 gacaatatac gcatccggct gggtttcttt cgccgcttcg gtgatcccgg caacgtgctg
    27961 caaattattg cgcgccccac ccgcttcccc cagcatatgc accacatcca gccgccagcc
    28021 atccatattc cacggcgctt tcagccagtg gcggacaata ctgtcttccc cgcgataaat
    28081 ttcattcacc agactttccg actgataatc cagcttcggc aagctggcat agcccagcca
    28141 gtcgagcgcc gtgccatcat cactaaacga gtaccagtca cgccagggtg attcggggtt
    28201 gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac caggcatggg aatcgccact
    28261 gtggttaaac acgccgtcca gcaccagtcg tattcccagc tgctgcgtat tgtgtcgcaa
    28321 acgcagcaac gccccatcac cgccaaactg cggatcgaca tggcgataat cctcggtatc
    28381 gtatttatgc acgctgggag ctttaaacac cggattgaga tacagcgccg tcacgccaag
    28441 ttttttcaga tacggcagtt tttcacttat cccgtccaga tcgccgccat agaacgttga
    28501 tccgccagcc tgcgccgtga ccggttcatc ccagtcacgc aatatgatct cttgtccggc
    28561 tgcgtgatgg taatagacat gatcctgttc agcttcacga ggaagattac gggcgaaacg
    28621 atcagggaag atctgataaa aaatctgatc cgcagcccat tgtgggccga tatccggtac
    28681 atcaatggca aactgctcca gtcgtgctgg cggcattcgg ctgaagcctt gcggtgtaaa
    28741 ccaacgctgg cgatcgtgcc acagcaattt gaaactataa cgccgccgag gttgtccgct
    28801 ggagagatcg atcgccgccc gccatgcggt gacgccaggc tgcggctgac tgcgctgctt
    28861 atgcatcggt actgacattt cttcgttatc gtgttctgta cgcagcataa tgcgctgcgg
    28921 tgggtcttcg ctcgtcagcc acagtgtaat gagcagttga tctttgcttt gtttaacaaa
    28981 tgggggcacc ggcagatgcc atgcatttaa catcataatt cccctgtgat gaaaatctga
    29041 tcaccttgcc atagcctaat gcaggatcac tcatcatctg aaggatattt gggggaggag
    29101 gacgggggcg gataactttc ggaatagccg gatcaggatt tcctcctgaa ccggcttaca
    29161 gaacgcaagg attactggtt ttcagccagc tgacgatcgt ggcggcgctt aaacatccag
    29221 ccaatcaaca gcacaacaat ccacgcgaaa ccaacataca gcgagatacg cgtatcaggg
    29281 tgataaccaa tcaacccgat aataaagagc aggaaaatca aaccgccgat ggtggttgcg
    29341 accccacccg gcactttaaa tttcagcgcc ttaacttctt ctggcggcaa acggcgacgg
    29401 aaggcgattt gcgacagcag gatcataatc cacacccaca ccgtggcgaa ggttgccagc
    29461 gaagcgatca ccaggaagac gttttccggc atgatgtagt tcagatacac cgcaaacagc
    29521 agcgcggtag tcatcaccag caccgtaacc cacggaatac cgcgacgcga ggttttgctg
    29581 aaaatttttg gcgcgctgcc ctgctctgcc ataccgtgga gcatacggcc tacaccaaat
    29641 acgtcactgt taattgccga cagcgaagcg gtcagcacaa caaagttaag aatgctggcg
    29701 gcaaaggtaa tgcccatatg ctggaacgtc agcacaaacg ggctaccggc agtgccaacc
    29761 tgattccacg ggtagataga cataatgacg aacagcgtac cgacgtagaa caccagaata
    29821 cgcatcggca cggagttaat cgcacgcgga atcgatttct caggatcttt cgcttcaccg
    29881 gcggtaatcc cgataatttc gatcccaccg taagcaaaca tcaccatttg caacgacatc
    29941 accataccaa gccagccgtt actgaagaag ccgccgttgc tccacaggtt atgaataccg
    30001 gtcggttgcc cgccgttgcc aatcccccag atgatgatgc cgaaaccggc gacaatcatg
    30061 atgatgatag tggcgacttt aaagaacgag aaccagaact ccagctcacc gaataccttc
    30121 acgctcatca ggtttacggc gcaaatgatc agcaccacac tcagtaccca aatccagtgc
    30181 ggcaccgtcg ggaaccagac acccatatag ataccaaaag cggtcacatc ggcgatggcg
    30241 acaataagga tttcaaagca gtaggtccag ccggtaatgt aacctgccag cgggccgagg
    30301 ttttcctgcg cataacgcga gaaagagctg gctgccgggt tatgtaccga catttccccc
    30361 agcgcacgca taatgatata cgccgcgata ccaccgataa tataggccaa caacacgctt
    30421 ggaccggcca ttttgatggc atctgccgaa ccgtaaaaca gcccggtgcc aattgctgaa
    30481 cccagtgcca taaagcgtat gtgtcgggta cttagcccac gctttagctt gttcttactt
    30541 tccatcaatt cctaacccat caacacaata aaaaaccacg gggcatcgca gccccgtggt
    30601 taaaacaaat gttcagtgat ttagtgagcg ctggaggtca cctgacgacc tgccgcacga
    30661 tcccagataa tggccagaac caccatcacc actgttggca ttaaccacgc cagaccttgt
    30721 tcggccagcg gtaaacgctg cgcccaagac ggtaagatat cgctgaatgc agatgccttg
    30781 atcccgtcga gaataccaaa aagcaggctg ataaacatcg gcggagcaat cacgcgggac
    30841 gaattatgcc accatgagcg tgtaaaactt aatacaacca gtgcgataca cggcggataa
    30901 atggcggtca gcaccggtac agagatctga atcagctggc tcaagccgag gttagacacc
    30961 accatcgaga agccgccgag gataaacacc agcgtacgat aagagagcgg tacgtactgg
    31021 gcgaagaatt ctgcacaagc acaggtcagg ccaaccgccg tgaccaggca ggcgatgaag
    31081 attaacgccg ccagcaggaa gctaccgccg ccgccaaagg tatgctgaac gtaagcatgc
    31141 aggatcgccg caccgtttgc agactgatcg accagcgacg cgctgtctga acccagacgg
    31201 aacagcgcca ggtacagcag agtcagacca acacccgcca tcaggccagc ccagacggta
    31261 taacgggtca gcagacgcgc ttcggtaacg ccacgagaac gcgccgcgtt aacaataacg
    31321 ataccaaaca ccattgcgcc cagcgtatcc atggtcagat agccgttgac gaagccgtta
    31381 gaaaacgcag cgttttgata agcctcagtc gccgtgctga tagaacccgc cggccagata
    31441 attgcggcaa cagacaggat gaccagcgcg ataattttca gcggcgcaag gaagttgccc
    31501 acggtatcca gcagcttgcc cggatagagc gaaaccagaa taacgatagc gaaatagacc
    31561 aggctgtaaa taaacagcgg cagcgcggaa tcacccgtca gcggcgcaat gcccacttca
    31621 aaagaaacgg tagctgtacg cggcgtagcg aaaagcggcc ccaccgccag gtaacaaact
    31681 gttgccagca gtacgccagc gactttacca attggcgtgc tgagactgtc aacaccgccg
    31741 ccaacttttg ccagcgccac taccgttaat accggtaggc caacggcagt aatgaggaag
    31801 ccgaatgccg cagtccagac gtgttcgcct gcctgcaagc cgaccattgg agggaaaata
    31861 atgttacctg cgccgacgaa caacgcaaat gtcataaagc ccagagcgat gatatcgcgc
    31921 gatcttaatt gatgggtcat aaatcatact gcctgtggat gtggtgttgt aacgttaaat
    31981 ttttgtgcca atccctgggg atgatacagc ggtatattta aacaatttta gcgggaaata
    32041 ctcccaacca ggcgcggcga agaatagttt ttcgatttac cgcgtaaatg cagtcagtat
    32101 gacagcacct gaagcgcaat ttaaacgctt ataacgtttt aaagcaagaa gggatctaaa
    32161 aaccagaggt atatagcgat aagaaatcac caaccagtat aaaacactat ttcaatgaaa
    32221 aagacatggc caatcatgcg aacaaaaaat caccaaccgt tgtaaaatct aacgcccgta
    32281 tttcgactgc aaagaaaata agccagcatc ggctggctta tggcaataaa agatgacaaa
    32341 ggcgaattaa tcgctgtttt tggcaattaa acgttccggg ataacaaaac tgaaacgagt
    32401 accttttcct acagtactct caatattcag gcgactttcg tgatgattca ccgcatgttt
    32461 cacgatcgct aaccctaatc cgctaccacc ggtttgccgg gaacgcgctt tatcaacgcg
    32521 ataaaaacgc tcggtcaggc gcggaatatg ctccggtgca atgcccggtc cgttatcttc
    32581 aacactaaat tcggcaccgt gcggcactcg ctgccagcgt acggtgatat gcgtgccttc
    32641 cggcgtatga ttcacggcgt tatagaccag gttcgaaatc gcactgcgta gctgatcttc
    32701 gttgcctgac accttgaggc cgttatctat ctcaaaagta aatgtctgtt ttttctgact
    32761 cagagtctga gcctcgcgct caacgacgcg cagcatcatc ggcacatcaa ccttttcatt
    32821 gagcaaatgc gtcggtgcgg cttctatttt cgacagcgtc agcaattgct tcaccaaccc
    32881 ttccatccgc tgggtctgct cgcgcatggt gtgcaacgct ttttcacgta ccgcgccttc
    32941 cagcggttgc tcatccatca tctccaggta accctgtaac acggtcaatg gcgtacgtaa
    33001 ctcatggctc acgttggcaa aaaagttacg ccgcgcccct tccagttgat gcatttgcgt
    33061 gacatctcgc gccaccatca gcaactgttt gtgggtataa ggcatgacgc gaatctccag
    33121 atgccgcccg gtattgagca ccagattgag cgggcgagag aaatcacgcg ttttcagata
    33181 ttgcgtaaac tccgggtaac gcagcaagtt aaggatgttc tgcccgttat cttccggcca
    33241 gcgcaagcca agaatctgtt gcgccagacc gttgcaccag aaaataccgc cctcttccgt
    33301 ggtcagcacc accgcgtcgg gcagcgactc cgcgccgcta cgaaagcgtt taatcagatt
    33361 gcccagttca cggcggcgtt ttttatttcg cagctgcatc tggtgtaagc cgtatagtag
    33421 cggttcccag ctaccacgcc ccggtggcgg ggtcatacta cgatccaccc acagccacca
    33481 tgaaaggcgc aataaattcc agaaatgcca gataagcagt cctgttaccg atgccagcaa
    33541 aaaccagggc aggtaaccaa aaaatgcacc caggatgaaa gccgggaggc agcaaagtag
    33601 cagctccagc accagccttt tccacgacag ccgttccagc acgtaagata ctccagttaa
    33661 gaaatcataa gccctgctct acgtccgatg agcaaggcct taaaagcggg ttgaaaaacg
    33721 ataccctgta ccgcgcacgg tctgcaccat gcggtcatgc ccgccgggct ccagtgcttt
    33781 acgcaggcga cgaatgtgga catcgaccgt acgatcttcc acatacacgt tggttcccca
    33841 tacgtgattt aacagttgct cgcgactgta cacgcgctca ggatgcgtca taaaaaagtg
    33901 cagcagttta aattctgtcg gccccatctc cagcggttct tcgcccgcca tcacccggtg
    33961 agatgtcggg tccagactta gcccctgcat ctcgatcacc tcttccaccg ccattggcga
    34021 aatacggcgc attaccgctt tgattcgcgc caccagctcc ttcggagaaa acggcttggt
    34081 gatatagtca tccgcaccgg tttcaaggcc gcgcacgcga tcttcttctt cccctctggc
    34141 ggtcaacatc accactggaa tatcccgggt catcgactcg cgcttgaggt gtttgatgaa
    34201 ctggataccg gagccgccag gtaacatcca gtcgaggaga attaaatccg gccagggttc
    34261 attcagttga ttcacagcac tgtcataatc ttccgcttcg accggctgaa agccattttg
    34321 ttcgagcacg aagcagacca tttcgcgaat tggagcttca tcttctacga ccagaatacg
    34381 tctcgccatg atttgccctg ttgtaataaa taggttgcga tcattaatgc gacgtcatta
    34441 tgcgtcagat ttatgacaga tttatgaaaa gctcgtcgca catcctttca ggttattgat
    34501 ttccgtggcg cagaaaaaag caaatggcac atctgtttgg gtataatcgc gcccatgctt
    34561 tttcgccagg gaaccgttat gcgcatcctt cacacctcag actggcatct cggccagaac
    34621 ttctacagta aaagccgcga agctgaacat caggcttttc ttgactggct gctggagaca
    34681 gcacaagccc atcaggtgga tgcgattatt gttgccggtg atgttttcga taccggctcg
    34741 ccgcccagtt acgcccgtac tttatataac cgttttgttg tcaatttaca gcaaactggc
    34801 tgtcatctgg tggtactggc aggaaaccat gactcggtcg ccacgctgaa tgaatcgcgc
    34861 gatatcatgg cgttcctcaa tactaccgtg gtcgccagcg ccggacatac gccgcaaatc
    34921 ttgcctcgtc gcgacgggac gccaggcgca gtgctgtgtc ccataccttt tttacgcccg
    34981 cgtgacatta ttaccagcca ggcggggctt aacggtattg aaaaacagca gcatttgctg
    35041 gcggctatta ctgattatta tcaacaacac tatgccgatg cctgcaaact gcgcggcgat
    35101 cagcctctgc ccatcatcgc cactggacat ttaacgaccg tgggtgccag taaaagtgac
    35161 gccgtgcgtg acatttatat tggcacgctg gacgcgtttc cggcacaaaa ctttccacca
    35221 gccgactaca tcgcgctcgg gcatattcac cgcgcgcaga ttattggcgg catggaacat
    35281 gttcgctatt gcggttcccc cattccactg agttttgatg aatgcggtaa gagtaaatat
    35341 gtccatctgg tgacattttc aaacggcaaa ttagagagcg tggaaaacct gaacgtaccg
    35401 gtaacgcaac ccatggcggt gctgaaaggc gatctggcgt cgattaccgc acagctggaa
    35461 cagtggcgcg atgtatcaca ggagccgcct gtctggctgg atatcgaaat cactactgat
    35521 gagtatctgc atgatattca gcgcaaaatc caggcattaa ccgaatcatt gcctgtcgaa
    35581 gtattgctgg tacgccggag tcgtgaacag cgcgagcgtg tgttagccag ccaacagcgt
    35641 gaaaccctca gcgaactcag cgtcgaagag gtgttcaatc gccgtctggc actggaagaa
    35701 ctggatgaat cacagcagca acgtctgcag catcttttcg ccacgacgtt gcatagcctc
    35761 gccggagaac acgaagcatg aaaattctca gcctgcgcct gaaaaacctg aactcattaa
    35821 aaggcgaatg gaagattgat ttcacccgcg agccgttcgc cagcaacggg ctgtttgcta
    35881 ttaccggccc aaccggtgcg gggaaaacca ccctgctgga cgccatttgt ctggcgctgt
    35941 atcacgaaac cccgcgcctc tctaacgttt cacaatcgca aaatgatctc atgacccgcg
    36001 ataccgccga atgtctggcg gaagtggagt ttgaagtcaa aggtgaagcg tatcgcgcgt
    36061 tctggagcca gaatcgggcg cgtaaccagc cggacggtaa tttgcaggtg ccacgcgtag
    36121 agctggcgcg ctgcgcagac ggcaaaattc tcgccgacaa agtgaaagat aagctggaac
    36181 tgacggcgac gttaaccgga ctggattacg ggcgcttcac ccgttcgatg ctgctttcgc
    36241 aggggcaatt tgctgccttc ctgaatgcca aacccaaaga acgcgcagaa ttgctcgagg
    36301 agttaaccgg cactgaaatc tacgggcaaa tctcggcgat ggtttttgag cagcacaaat
    36361 cggcccgcac agagctggag aagctgcaag cgcaggccag cggcgtcgcg ttgctcacgc
    36421 cggaacaagt gcaatcgctg acagcgagtt tgcaggtact tactgacgaa gaaaaacagt
    36481 taattaccgc gcagcagcaa gaacaacaat cgctaaactg gttaacgcgt ctggacgaat
    36541 tgcagcaaga agccagccgc cgtcaacagg ccttgcaaca ggcattagcc gaagacgaaa
    36601 aagcgcaacc tcaactggcg gcgctcagtc tggcacaacc ggcacgaaat cttcgtccgc
    36661 actgggaacg catcgcagaa cacagcacgg cgctggcgca tactcgccag cagattgaag
    36721 aagtaaatac tcgcttacag agcacaatgg cgcttcgtgt gagcattcgc caccacgcgg
    36781 cgaagcagtc agcagaatta cagcagcagc aacaaagcct gaatgcctgg ttacaggaac
    36841 acgaccgctt ccgtcagtgg aacaacgaac tggcgggttg gcgtgcacag ttctcccaac
    36901 aaaccagcga tcgcgagcat ctgcggcaat ggcagcaaca gttaacccat gctgagcaaa
    36961 aacttaatgc gcttgcggcg atcacgttga cgttaaccgc cgatgaagtt gctaccgccc
    37021 tggcacaaca tgctgagcaa cgcccactgc gtcagcgcct ggtcgcactg catgggcaga
    37081 ttgttcccca acaaaaacgt ctggctcagt tacaggtcgc tatccagaat gtcactctgg
    37141 aacagacgca acgtaatgcc gcacttaacg aaatgcgcca tcgttataaa gaaaaaacgc
    37201 aacaacttgc cgatgtgaaa accatttgcg agcaggaaac tcgcattaaa acgctggaag
    37261 ctcaacgtgc acagttacag gcgggtcagc cttgcccact ttgtggttcc agcagccacc
    37321 cggcggtcga ggcgtatcag gcgctggaac cgggcgttaa tcaggcccgg ctattaacgc
    37381 tggaaaaaga agtgaaaaag ctcggcgaag aaggtgcggc gctacgtggg caactggatg
    37441 cattaacgaa gcagcttcag cgcgatgaaa acgaagcgca aagcctccga caagatgagc
    37501 aagcacttac tcaacaatgg caagccgtca cggccagcct caatatcacc ctgcagccac
    37561 aggacgatat tcaaccgtgg ctagatgcac aagatgagca tgaacgccag ctgcggttac
    37621 tcagccaacg gcatgaatta caagggcaga ttaccgcgca taatcagcaa attatccagt
    37681 atcaacagca aattgaacaa cgccagcagc aactcttaac ggcattggcg ggttatgcgc
    37741 tgacattgcc acaggaagat gaagaagaga gctggttggc gacgcgtcag caagaagcgc
    37801 agagctggca gcaacgccag aacgaattaa ccgcgctgca aaaccgtatg cagcagctga
    37861 cgccgattct ggaaacgttg ccgcaaagtg atgaactccc gcacagcgaa gaaactgtgg
    37921 cgctggaaaa ctggcggcag gttcatgaac aatgtctcgc attacacagc cagcagcaga
    37981 cgttacagca acaggatgtt ctggcggcgc aaagtctgca aaaagcccag gcgcagtttg
    38041 acaccgcgct acaggcgagc gtctttgagg atcagcaggc gtttcttgcg gcgctaatgg
    38101 atgaacaaac actaacgcag ctggaacagc tcaagcagaa tctggaaaac cagcgccgtc
    38161 aggcgcaaac gctggtcact cagacagcag aaacgctgac acagcatcag caacaccgac
    38221 ctggcgggtt atctctcact gtgacggtgg agcagattca gcaagagtta gcgcaaactc
    38281 accaaaagtt gcgtgaaaac accacgagtc aaggcgagat tcgccagcag ctgaagcagg
    38341 atgcagataa ccgtcagcaa caacaaacct taatgcagca aattgctcaa atgacgcagc
    38401 aggttgagga ctggggatat ctgaattcgc taataggttc caaagagggc gataaattcc
    38461 gcaagtttgc ccaagggctg acgctggata atttagtcca tctcgctaat cagcaactta
    38521 cccggctgca cgggcgctat ctgttacagc gcaaagccag cgaagcgctg gaagttgagg
    38581 ttgttgatac ctggcaggct gatgcggtac gcgatacccg taccctttcc ggcggcgaaa
    38641 gtttcctcgt cagtctggcg ctggcactgg cgctttcaga tctggtcagc cataaaacgc
    38701 gtattgactc gctgttcctt gatgaaggtt ttggcacact ggatagcgaa acgctggata
    38761 ccgcccttga tgcgctggat gccctgaatg ccagtggcaa aaccatcggg gtgattagcc
    38821 acgttgaggc gatgaaagag cgtattccgg tgcagatcaa agtgaagaag atcaatggac
    38881 tgggctacag caaactggaa agtgcgtttg caatgaaata actattcagc aggataatga
    38941 atacagaggg gcgaattatc tcttggcctc gctggtcgtt atcctgcaat ctatcacttt
    39001 attggctacg gtgattggta gccgttctgg tggttgtgat ggtggtatga aaaaagttat
    39061 tttatctttg gctctgggca cgtttggttt ggggatggcc gaatttggca ttatgggcgt
    39121 gctcacggag ctggcgcata acgtaggaat ttcgattcct gccgccgggc atatgatctc
    39181 gtattatgcg ctgggggtgg tggtcggtgc gcctataatc gcgctttttt ccagccgcta
    39241 ctcactcaaa catatcttgt tgtttctggt ggcgttgtgc gtcattggca acgccatgtt
    39301 cacgctctct tcgtcttacc tgatgctcgc cattggtcgg ctggtttccg gctttccgca
    39361 tggtgcattt tttggcgtcg gcgcgatcgt gttatcaaaa attatcaaac ccggcaaagt
    39421 caccgccgcc gtggcaggga tggtttccgg gatgacagtc gccaatttgc tgggcattcc
    39481 actgggaacg tatttaagtc aggaatttag ttggcgttac acctttttgt tgatcgctgt
    39541 ttttaatatt gcggtgatgg catcggtcta tttttgggtg ccggatattc gcgacgaggc
    39601 gaaaggaaaa ctacgcgaac aatttcattt tttacgcagc cctgccccgt ggttaatttt
    39661 cgccgctacg atgtttggca acgcaggtgt gtttgcctgg ttcagctacg taaagccata
    39721 catgatgttt atttccggtt tttcggaaac ggcgatgacc tttattatga tgttagttgg
    39781 gctagggatg gtgctgggga atatgctaag tggcaggatt tcaggacgtt attcaccact
    39841 gcgcattgca gcagtgactg actttatcat tgtactggca ctgctgatgc tctttttctt
    39901 cggcggtatg aaaacaacgt cgcttatttt tgcttttatt tgttgcgcgg gattatttgc
    39961 cctatcagca cctctacaaa tattattact gcaaaatgcc aaaggcggag agttattagg
    40021 tgccgcaggt gggcaaatag cgtttaacct cggtagcgcc gtcggcgcat attgcggtgg
    40081 tatgatgctg acgctggggc tggcatataa ttacgtggcg ctgcccgctg ccctgctttc
    40141 gtttgctgcg atgtcgtcgt tgctgctgta tggtcgctat aagcgccagc aagcggcgga
    40201 tagttcggtg ctggcgaaac cactggggta gatagcagtc gcagtgcgca tcgcttgttg
    40261 ccggatgcgg cgtgaacgcc ttatccggcc tacgggactg ttcattttgt aggcctgata
    40321 agacacgcca gtgtcgcatc aggcatcgga gcactggggg gttgccgcca ctgtgcgcgt
    40381 tatttgttgt cggatgcggc gcgagcgcct tatccggcct accggacggc tcactttgta
    40441 ggcctgataa gacgcggcaa gcgtcgcatc aggcatgata cgccaattgc ctggtttttt
    40501 tactcttgtg gccataacca tgcagcgccg cgtacgccgc tcgaatcacc atgtttcgcc
    40561 ttgcgtaccg gcgtttcaca ttctccgccg aagacaaatt gtttaatcaa ctgtggaacc
    40621 gtttgatata aacggtctac attgctcatt ccgccgccca ggacaatcac atccggatcg
    40681 agaatattca cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc
    40741 agttccgcta ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca
    40801 tgtccgctca aacgacgata atccgtcgcg aatcccgtgc ctgaaataaa ggtttcaata
    40861 caaccttgtt taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc
    40921 atccacggta gcggattgtg tccccactcg cctgccgtgc cattgccgcc gatatgcgcc
    40981 cgcccattga atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc
    41041 tgcgctcccg ctgccgcgcc atctaccgct tctgaaaccg ccagacagtt ggcgtcattt
    41101 gccagccgca cttcccgctg caaccgcgcg cttaagtctt tatcgaatgg ctgaccgttg
    41161 agccaggttg aattggcatt cttcaccacg ccggtgtagg gcgaaatcga acctggaatg
    41221 cccataccta ccgttccgcg ctgccctgtt gcctgctccg ccatatcaac caacgtggcg
    41281 atcgtttcaa tagtctgcag gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac
    41341 tgctcccctg catcgcccaa tgcaatcact tcagttttgg tgccgcctaa atcgatacct
    41401 atacgcacgg tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga
    41461 ttggcaatgc aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc
    41521 gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt
    41581 tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc
    41641 agccaggaca tggcgaagat gggctgggta cctccgatgg gatcgcacag cgatgcgtta
    41701 acgcacgttg ccaatggtca aattgttatc tgtgcgcgca aagaagaaaa aatcctcccg
    41761 tccccggtga ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg
    41821 cgtaagctga agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg
    41881 ccgcgtgctt tcagccgttt tagccagaca atgatgtgga tcgacacggt taacggtttg
    41941 attatggtgg actgcgccag cgccaaaaaa gcggaagata cgctggcact gctgcgtaaa
    42001 agcctggggt cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg
    42061 accgaatggg tacgttccgg cagcacggca cagggtttcc agctgcttga tgaagccgag
    42121 ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc
    42181 gaagagatca ccaaccacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg
    42241 cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct gaagttctgc
    42301 gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat
    42361 gccgatttca tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga
    42421 ttaggtggcg aagcacaacg ttaattgctg atttaccttt aatgccggat gcgacgcctg
    42481 ccgcgtctta tccggcgtac gaagccacac caggaatata atcgttagct acagcgggca
    42541 ataattctta gcgcagcaat attatgcgtt ttatgctgta acttgctcca ggggcgttgt
    42601 gtcattgttt ttcctcaagc cggtgacgcg cttcactgcg aatttttcgc gttgccccgt
    42661 ttttcactaa atcacgtaac agcgaaaaat cgggttgttc gacaaataag agtaatgatg
    42721 gctcctcttt taaccacgtc gttttcacat ccgcagccag ctgtgaatta ttaatagcca
    42781 gcgaccgctc ctgaggctca gtcaatgtcg ttaacaacac cggagtatgt gcgttgtgca
    42841 atgccgtttg gcgcaaatga tcattgcgac tttgcagcaa tgcacaccac cattcatcat
    42901 ctgatttatc ttttaacgtc gccagttccg caatagtatc tggagatctt cgctgatatt
    42961 tatatcaacc ttgatgaatt atgaatatat cggaaatcga aaaaatccca gcaaaggaaa
    43021 tattattatg ggttaaagag aggacatttc agaaaaatgc cctcccgttc aaggggaagg
    43081 cgaggaatta cagatagcgg cacagataag aggtgggttc ggcaacttgc agatgaaact
    43141 cgctgtgacc gggaacatta aacaccgaac cggcttcata cacttgccag tcggtcgcgt
    43201 caggcagtaa cacattcagc gcgccactga ttaccgtcat ctcttcaggc tcagcggtgc
    43261 tgaaggtgta ttcaccttca accataacac ccacgctggc gcgaccagtg ctgctgctgg
    43321 aaaagccgat cgatttcact ttgccggaaa agtactcatt actttgaagc ataaactggc
    43381 cctgtctggt agttgaacat ctacttcact ataggggcca gaggcgctgg ctgtcacgca
    43441 aaattacatc agtaattccg cagccagccg tgcaatcaat acgttagaga gcaagacagg
    43501 aacatcgagc tgtttttgca gtaaatcgcg atgacgctgg ttaaatccca aacaatccag
    43561 catgatgaca tctgcacctt ttgccagtaa ttctttcccg gcatcaatga ttttttgttc
    43621 cgaatcatga atgggattac ccaatgaaaa tactggcggt ttctgcaaaa tttgccattt
    43681 ttgcgcctga acggtcagca actcctcaac cggtacgata acccccacct gatgatcttc
    43741 aacaatagag gaaaccagcg gtggcaatat ccgcgacggc tcaagaaaga tcgtattacg
    43801 cgcggtcata ctactaatgt ttgctgtact cattaatata atgacgtcgt aaccctgatt
    43861 atcgagcact tcaaccacac cttgcaggtc tcgctccact ttgcgacgcg aaacatgggc
    43921 cagctggttg tcatttaata atgtgagaat ggtgtcttcg cctgcttctg gcgcgtactc
    43981 cgccatcact tcttcgcggc ttaacttccc cagcaggcta tgatgggaaa tattatcttc
    44041 gtcaatgtat tctgtcagga gcggcaaaac ttcctgcata ggtacaatgc cgatagtcag
    44101 gatcgccaac gacgcactca ttgttcttcc accttacttt tatttcgtcg ttaattaccg
    44161 ggcaagtgtt aagcaccact gctgacactt acttgtgcta caaatgacaa aaagcgtagc
    44221 agcagacctg cactgcataa tgtaaagatt ccaaatgatt ccagtaatgg attgttattg
    44281 tttaatattc taattatttg aaaaacatga attgtgaaaa atgtgacgca gatcccttgt
    44341 ggcggcactc agaatccctt ccgagcagtc tgatgtggtc actctttgtc agggtcctca
    44401 tagcgttttt tcgcatccat cgcttcctga gcggtcggat gttcactgat caacgagtct
    44461 ggtttaggat gatcggctct gagttggtac caggttaccg tctgtcctgg ctttcctttt
    44521 tcaatcgtca ctatatatgc ttcacgagga taaggcggtt tcgttggcat cgttcttcct
    44581 tatttcacgg gatgaacgtt aagtataggc gctcgaaaat caacaattga tcgtctgtgc
    44641 cagagcgctg cgaatttcag aaatcacctg gctgggttcg tttgttgcgt cgatgataat
    44701 atgcgcaact tcgcgatata gcgcatcgcg ttcttccagc acttcctgaa cttcttcgct
    44761 cagtggtttt cccgttaagg ttggccgtaa atcttcttcc ggtgcagctt gcagtcggtt
    44821 aaccaggact gatactggcg cacacaaata aaccacgatc ccgttatttt gcatgaagtg
    44881 acgattaaat tccgtcagaa taatgccgcc gcccgtggcg ataacggtgg atggcgcagt
    44941 taccgcttcc agtgccgccg tttctctggc gcggaatccc gcccactctt ccctttcgac
    45001 gatctccgcg accgtcatat tgagctgtga ttgcaaccac tggtcggtat cgacaaaccg
    45061 acggttaagc gaatcggcaa gggccattcc gaccgttgtt ttaccacagc cccgaggccc
    45121 gatcagaaaa agaggttgtg tcatcgtggg ttttccccaa taggtcgcaa tgcggcgaaa
    45181 gccggtgtca tgagaatagc gatcatacca tcaaactagt acaatttcga ttgtaaagaa
    45241 aaaattccac ttaaagtgaa aatctcaata catcccttac tataccaata aatattcaag
    45301 aatgaagtgt aaataataaa ttacatttag ccacgactac gttgcacttc cagccaccac
    45361 ttctccagct ccgcagcaaa agcctggcgg tcacgttgtg aaagactatc tggtcctccg
    45421 gtctggatcc cactggcacg taaggtatcc ataaaatcgc gcatcgtcag gcgctcacga
    45481 atggtcgctg gcgtgtaacg ttcgccgcgc ggattaagcg ccgccgcgcc tttctcgatg
    45541 gcttcagcag ccaaaggtat atctgcggtg atcaccaaat cgcccgcttc acactgccga
    45601 acaatttcgt tatcggcaac gtcgaaacct gccgcgacgc gcagcgtacg aataaatcgc
    45661 gatggcggca cgcgtaaact ctggtttgct accagtacca gcggcatctg catacgttcc
    45721 gccgcgcgat acaaaatctc tttaattaca ttgggacacg cgtcggcatc cacccaaatt
    45781 gtcataaagt catcctttgt tgggtaatcc tctattgtgt cgcgcttttg ccttccggca
    45841 tagttctgtt tatgcttctg ccagcgatta tcaaaacaat gaatttcacg gcaggagtga
    45901 ggcaatggaa aagaaaatcg gttttattgg ctgcggcaat atgggaaaag ccattctcgg
    45961 cggtctgatt gccagcggtc aggtgcttcc agggcaaatc tgggtatata ccccctcccc
    46021 ggataaagtc accgccctgc atgaccagtt cggcatcaac gccgcagaat cggcgcaaga
    46081 agtagcgcaa atcgccgaca tcatttttgc cgccgttaaa ccaggcatca tgattaaagt
    46141 gcttagcgaa atcacctcca gcctgaataa agactctctg gtcgtttcta ttgctgcagg
    46201 tatcacgctc gaccagcttg cccgcgcgct gggccatgac cggaaaatta tccgcgccat
    46261 gccgaacact cccgcgctgg ttaatgccgg gatgacctcc gtaacgccaa acgcgctggt
    46321 aaccccagaa gataccgctg atgtgctgaa tattttccgc tgctttggcg aagcggaagt
    46381 gattgctgag ccgatgatcc acccggtggt cggtgtgagc ggttcttcgc cagcctacgt
    46441 ctttatgttt atcgaagcga tggccgacgc cgccgtgctg ggcgggatgc cacgcgccca
    46501 ggcgtataaa tttgccgctc aggcggtaat gggctccgca aaaatggtgc tggaaacggg
    46561 agaacatccg gggacactga aagatatggt ctgctcaccg ggaggcacca ccattgaggc
    46621 ggtacgcgta ctggaagaga aaggcttccg tgccgcagtg atcgaagcga tgacgaagtg
    46681 tatggaaaaa tcagaaaaac tcagcaaatc ctgatggctt ttgccggacg tcaggccgcc
    46741 acttcggtgc ggttacgtcc ggctttcttt gctttgtaga gcgccaaatc tgccgatttc
    46801 aaccactcgc gatagtgact catttgtgga ttcagcggcg caacccccac actaatccgt
    46861 aaagttacct gtggcgtatt cggcaagcgt aacgtgttta gcccttcatg cacccgtaac
    46921 atggcggtga tggcgctctc agctggcgta ccggacatga ttactgcaaa ctcatcgccg
    46981 ccaaaccgac caatcacatc gctaccgcgc agggtaattt gtaactgtcg ggtaagcgcc
    47041 acaatcgctt catcacccac atcatggccc caggtatcgt tgatgctctt gaaatggtcg
    47101 atatcgataa tcagtaacgt tgcatcgcga ttatgccgcc gacagttatc aaattcattg
    47161 cgtaacatag tttcccaatg acgtcggtta tacacgcccg tcataccgtc gcgggtactc
    47221 atgacctgca acctgcgttt atgttccgcc agtttagtcg ccgtctggta gctgacccag
    47281 ccaaacagca gaggataaat gacaataatg ggaagggaga gccaccattc cagcggtgcg
    47341 ctattgaacg acacggtgat gcccgtcagc tcgagggtga caaggcagga gaccaccatc
    47401 aacaccagac ccgcgacaaa cagacggggg ccgcctgccc ccatcaaatt cagacacata
    47461 atcatcaaca tcgcggtgga aggcagtgcg tttacgccca ttacgcctac ccacattccc
    47521 gctaatactg catcggtttt taagttgtaa atttcccggc taagcggatc gacggccctg
    47581 ctcgctattt gccaggctaa atgcggccag acgaacgccc agccgaccaa caacagccac
    47641 caccagcccg gcgacggatg cgaaaccagc gttgaagcaa tcggtaagaa catgccagcc
    47701 aggccaaccg cgcggggtag tctgacgcga cgggcaaagc gcagcccgga ccgctgatgt
    47761 tcgttttgag gagttaaagg aggttcctcc ccatgcgccg ccgctttttt gaaaaagttt
    47821 tcatcattca ttatttttgg gaacattcag aaacaatttt cccaaattat agagacgggg
    47881 ctaaaggcag aagtatgaaa tttcgggtga gccattttcg tagctcaccc gttagcacta
    47941 tcaggtatca ggcggctttc ttgaggcagg cactcataaa cttattacga tcatcccctt
    48001 tcagagattg ttgtgttgct tgattattac attcgcgcat cttttgctgc tgtggcgtca
    48061 aacttttttc accaggcgca gatttgctgt tcttcaggca atcactcatg taggtcttac
    48121 gagcatcccc tttcaacgcc tgcgccgtcg cctgctgatt acaagaggtc atacgctgtt
    48181 gttgtggggt taaggttctc tcggcagcgc cgacggtggt taaaaaaacc agaccgaaaa
    48241 gcaaggtaac cagtaatgta attttcatcg caccatccct cttcatgttt taaccatgag
    48301 cgtatgcgcc cgtgatctgt cattaagtct ggttgctaac agcaaaaaaa ccacccggca
    48361 gcgaaaattc actgccgggc gcggttttat ttcagcccca gggcggcttt catggtgtag
    48421 aagagatcgg tctggtcggt cagtccaaca acattggcgg catgcgggcc atacgccgca
    48481 atacgcaact gactgccggt atgttcttgt gaatcctctt cggagttccc gtaactcatc
    48541 accatcactg cgccatcttt ggtatttagc gcctgggtga ggcccggagc tttggtatct
    48601 ggcgcaacta tctggctggc atgggcgtga tcagcggtga ctatgaccaa cgtgttacca
    48661 tcctttttag cgaattccag cgcccgttgt acggcttcat cgagatcgac cgtctcgcca
    48721 atttgcccac aaggattcgc agcgtgatcc tgtttatcga ttgacgcacc ttcaacttgc
    48781 aggaaaaagc ctttctcatt tttactcaac aattcaatgg ctttgtcggt catctgcgcc
    48841 agggtcggta cactgtcatt acgttgcgga ttaggcgtac aggtgactgc gggcttatct
    48901 atattaccgt ggtacgttgc tttcggtcct tgccagcgca ctggcatatt gccgtcagca
    48961 aacagtccta ataggggttt ttgctgattc gcttccgtca ccgaattcag tgaggcagca
    49021 tcgctcacca actgataacc acgcgcctgt gcctgttcac gcagcgtttt tccctgccat
    49081 tcaccggcgg ttgccgtttc agcaaaggtt tttgcgccgc cgccaagcgt aacatcggca
    49141 cgggcgttaa gcagctgttc ggtaatcgat ccttttccgc ctttttccag agcgttaccc
    49201 ggacattttt cactggtcgc actcggaccg tagcatttgc gcgaggtcac atgcgccacc
    49261 agcgcagcgg gcgtggcatc ctgcaactct gcggtagaaa cgttaccggt cgccagacct
    49321 gcggcttttg ccatttccag aatcgttggg tgatcttttt cgtgaatatc gacgcccagc
    49381 gcgccgttat aggttttgac accagttgac caggcggttg ctgatgcagc cgagtcggtg
    49441 acgtagtccg gtttgccggt ttttttattc agcgcatagt gagtgtattg cccggtaagc
    49501 ggtaaggcat cgataccttt aaaaaagccg cccgcacctt cggcataatt gcgtgcggca
    49561 gtaatttccg agtcccccat cccatcgcca atcagcaaaa taatattttt tgcaggttta
    49621 tcgctaagag aatcacgcag agcggcggtc tgatcacccg ttaagcggcg agcaccgccg
    49681 ggtgcagtaa tatcgctctg agcagcccgg ttttccagaa caggcatttc tggtgtccgg
    49741 gcttttgtca caggggtaaa cagtaacggt aagagtgcca gtgcaatagt gctttgtttc
    49801 actttatttt ctccatgtac aaatacatta aaaaataaaa acaaagcgac tataagtctc
    49861 ggccgtgaca actttatgac agctgttgaa aagattaact ttttactgac gaggatgcct
    49921 caataacttc tttacgtaat cgcgcagcag ctccgtatcg tcgtcaggaa tgctggcatc
    49981 gggctttacc tcgtacagcg ccccctctac ctgatcaatc aaccgctgtt gatcattttg
    50041 cgccatattg cgaagcattg ccgtgacgat aatctccaaa gcttctacct gcgcgcacag
    50101 ttctttcgac tcttcttctt tttgggcaag cttaaataac aactcagcaa tgagattttt
    50161 catgtctgta tttccttatc caa
//
LOCUS       DADSZD010000013        49868 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.013, whole genome shotgun sequence.
ACCESSION   DADSZD010000013 DADSZD010000000
VERSION     DADSZD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.013"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            83..1132
                     /locus_tag="J1Z28_000676"
     CDS             83..1132
                     /locus_tag="J1Z28_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBA7715536.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1206..2063
                     /gene="nfo"
                     /locus_tag="J1Z28_000677"
     CDS             1206..2063
                     /gene="nfo"
                     /locus_tag="J1Z28_000677"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBA7715537.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLHGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2067..3155
                     /locus_tag="J1Z28_000678"
     CDS             2067..3155
                     /locus_tag="J1Z28_000678"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7715538.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3423..4364)
                     /gene="rihB"
                     /locus_tag="J1Z28_000679"
     CDS             complement(3423..4364)
                     /gene="rihB"
                     /locus_tag="J1Z28_000679"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBA7715539.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGVTIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            4494..5192
                     /gene="yeiL"
                     /locus_tag="J1Z28_000680"
     CDS             4494..5192
                     /gene="yeiL"
                     /locus_tag="J1Z28_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBA7715540.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPEHKFSGMMQ"
     gene            complement(5259..6509)
                     /locus_tag="J1Z28_000681"
     CDS             complement(5259..6509)
                     /locus_tag="J1Z28_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBA7715541.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGALDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(6603..7541)
                     /locus_tag="J1Z28_000682"
     CDS             complement(6603..7541)
                     /locus_tag="J1Z28_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBA7715542.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(7529..8470)
                     /locus_tag="J1Z28_000683"
     CDS             complement(7529..8470)
                     /locus_tag="J1Z28_000683"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBA7715543.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(8926..10617)
                     /gene="fruA"
                     /locus_tag="J1Z28_000684"
     CDS             complement(8926..10617)
                     /gene="fruA"
                     /locus_tag="J1Z28_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBA7715544.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(10634..11572)
                     /gene="fruK"
                     /locus_tag="J1Z28_000685"
     CDS             complement(10634..11572)
                     /gene="fruK"
                     /locus_tag="J1Z28_000685"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBA7715545.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(11572..12702)
                     /locus_tag="J1Z28_000686"
     CDS             complement(11572..12702)
                     /locus_tag="J1Z28_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBA7715546.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            13070..14251
                     /gene="setB"
                     /locus_tag="J1Z28_000687"
     CDS             13070..14251
                     /gene="setB"
                     /locus_tag="J1Z28_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBA7715547.1"
                     /translation="MHNSPAVTSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(14248..14502)
                     /locus_tag="J1Z28_000688"
     CDS             complement(14248..14502)
                     /locus_tag="J1Z28_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBA7715548.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            14657..15229
                     /gene="yeiP"
                     /locus_tag="J1Z28_000689"
     CDS             14657..15229
                     /gene="yeiP"
                     /locus_tag="J1Z28_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBA7715549.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            15452..16918
                     /locus_tag="J1Z28_000690"
     CDS             15452..16918
                     /locus_tag="J1Z28_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA7715550.1"
                     /translation="MNTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQAIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQNPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPNNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATNESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLDE
                     QAPTLRIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLERE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAVSSSEQRVTALLSLRE
                     IFGDDLPDNPHFVQAIEQAWQQIAQFGAHQALLNTLKI"
     gene            17036..18022
                     /locus_tag="J1Z28_000691"
     CDS             17036..18022
                     /locus_tag="J1Z28_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBA7715551.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQHSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            18061..18774
                     /gene="lpxT"
                     /locus_tag="J1Z28_000692"
     CDS             18061..18774
                     /gene="lpxT"
                     /locus_tag="J1Z28_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBA7715552.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVIFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            19186..19752
                     /gene="mepS"
                     /locus_tag="J1Z28_000693"
     CDS             19186..19752
                     /gene="mepS"
                     /locus_tag="J1Z28_000693"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBA7715553.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            19933..21489
                     /locus_tag="J1Z28_000694"
     CDS             19933..21489
                     /locus_tag="J1Z28_000694"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBA7715554.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            21571..23385
                     /locus_tag="J1Z28_000695"
     CDS             21571..23385
                     /locus_tag="J1Z28_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7715555.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            23386..24480
                     /locus_tag="J1Z28_000696"
     CDS             23386..24480
                     /locus_tag="J1Z28_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBA7715556.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            24480..25505
                     /locus_tag="J1Z28_000697"
     CDS             24480..25505
                     /locus_tag="J1Z28_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715557.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            25507..27096
                     /gene="yejF"
                     /locus_tag="J1Z28_000698"
     CDS             25507..27096
                     /gene="yejF"
                     /locus_tag="J1Z28_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBA7715558.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIAAGETLALVGES
                     GSGKSVTALSILRLLPCPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHPLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEKNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PYARVFAAPQQEYTRQLLALS"
     gene            complement(27100..27444)
                     /locus_tag="J1Z28_000699"
     CDS             complement(27100..27444)
                     /locus_tag="J1Z28_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715559.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(27777..28967)
                     /gene="bcr"
                     /locus_tag="J1Z28_000700"
     CDS             complement(27777..28967)
                     /gene="bcr"
                     /locus_tag="J1Z28_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBA7715560.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(28995..29690)
                     /gene="rsuA"
                     /locus_tag="J1Z28_000701"
     CDS             complement(28995..29690)
                     /gene="rsuA"
                     /locus_tag="J1Z28_000701"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBA7715561.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            29840..31600
                     /locus_tag="J1Z28_000702"
     CDS             29840..31600
                     /locus_tag="J1Z28_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA7715562.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            31725..32009
                     /gene="rplY"
                     /locus_tag="J1Z28_000703"
     CDS             31725..32009
                     /gene="rplY"
                     /locus_tag="J1Z28_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBA7715563.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(32148..33155)
                     /gene="yejK"
                     /locus_tag="J1Z28_000704"
     CDS             complement(32148..33155)
                     /gene="yejK"
                     /locus_tag="J1Z28_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBA7715564.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            33337..33564
                     /locus_tag="J1Z28_000705"
     CDS             33337..33564
                     /locus_tag="J1Z28_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBA7715565.1"
                     /translation="MPQISRYSDEQVEQLLAELLNILEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            33584..35344
                     /gene="pbgA"
                     /locus_tag="J1Z28_000706"
     CDS             33584..35344
                     /gene="pbgA"
                     /locus_tag="J1Z28_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBA7715566.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            35419..35495
                     /locus_tag="J1Z28_000707"
     tRNA            35419..35495
                     /locus_tag="J1Z28_000707"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35453..35455,aa:Pro,seq:ggg)
     gene            complement(35606..36549)
                     /locus_tag="J1Z28_000708"
                     /pseudo
     CDS             complement(35606..36549)
                     /locus_tag="J1Z28_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247409.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            36869..37516
                     /gene="narP"
                     /locus_tag="J1Z28_000709"
     CDS             36869..37516
                     /gene="narP"
                     /locus_tag="J1Z28_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBA7715567.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(37551..38603)
                     /locus_tag="J1Z28_000710"
     CDS             complement(37551..38603)
                     /locus_tag="J1Z28_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HBA7715568.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(38600..39157)
                     /gene="dsbE"
                     /locus_tag="J1Z28_000711"
     CDS             complement(38600..39157)
                     /gene="dsbE"
                     /locus_tag="J1Z28_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBA7715569.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(39154..41097)
                     /locus_tag="J1Z28_000712"
     CDS             complement(39154..41097)
                     /locus_tag="J1Z28_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBA7715570.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFLRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLLDPRYRKRVNPQKTVPEAA"
     gene            complement(41094..41573)
                     /gene="ccmE"
                     /locus_tag="J1Z28_000713"
     CDS             complement(41094..41573)
                     /gene="ccmE"
                     /locus_tag="J1Z28_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBA7715571.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKGPAS"
     gene            complement(41570..41779)
                     /gene="ccmD"
                     /locus_tag="J1Z28_000714"
     CDS             complement(41570..41779)
                     /gene="ccmD"
                     /locus_tag="J1Z28_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBA7715572.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(41776..42513)
                     /gene="ccmC"
                     /locus_tag="J1Z28_000715"
     CDS             complement(41776..42513)
                     /gene="ccmC"
                     /locus_tag="J1Z28_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBA7715573.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(42555..43217)
                     /gene="ccmB"
                     /locus_tag="J1Z28_000716"
     CDS             complement(42555..43217)
                     /gene="ccmB"
                     /locus_tag="J1Z28_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBA7715574.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(43214..43831)
                     /gene="ccmA"
                     /locus_tag="J1Z28_000717"
     CDS             complement(43214..43831)
                     /gene="ccmA"
                     /locus_tag="J1Z28_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBA7715575.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(43850..44452)
                     /gene="napC"
                     /locus_tag="J1Z28_000718"
     CDS             complement(43850..44452)
                     /gene="napC"
                     /locus_tag="J1Z28_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBA7715576.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(44462..44911)
                     /gene="napB"
                     /locus_tag="J1Z28_000719"
     CDS             complement(44462..44911)
                     /gene="napB"
                     /locus_tag="J1Z28_000719"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBA7715577.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(44908..45771)
                     /gene="napH"
                     /locus_tag="J1Z28_000720"
     CDS             complement(44908..45771)
                     /gene="napH"
                     /locus_tag="J1Z28_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBA7715578.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(45758..46453)
                     /gene="napG"
                     /locus_tag="J1Z28_000721"
     CDS             complement(45758..46453)
                     /gene="napG"
                     /locus_tag="J1Z28_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBA7715579.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(46460..48946)
                     /gene="napA"
                     /locus_tag="J1Z28_000722"
     CDS             complement(46460..48946)
                     /gene="napA"
                     /locus_tag="J1Z28_000722"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBA7715580.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYDVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(48943..49206)
                     /gene="napD"
                     /locus_tag="J1Z28_000723"
     CDS             complement(48943..49206)
                     /gene="napD"
                     /locus_tag="J1Z28_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBA7715581.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(49196..49690)
                     /gene="napF"
                     /locus_tag="J1Z28_000724"
     CDS             complement(49196..49690)
                     /gene="napF"
                     /locus_tag="J1Z28_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBA7715582.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
BASE COUNT        12198 a        13383 c        12275 g        12012 t
ORIGIN      
        1 taaagattaa ttataaatat ataatcaatt ttatttttaa accagttagt cgtaacctta
       61 tgcccggtta aaggagaggg ttatgacgaa tatcacctta cagaaacaac atcgtacact
      121 gtggcatttt attccggggt tagccctgag tgcagttatc accggggtcg ccctgtgggg
      181 tggttccatt cccgctgttg cgggtgccgg gtttagtgcc ctcaccctcg caatcttgtt
      241 ggggatggtt ttaggcaaca ccatttaccc gcacatctgg aaaagctgtg acggtggtgt
      301 gctgtttgcc aagcaatatt tattacgtct gggtatcatt ctttatggct tccgtctgac
      361 gttctcgcaa attgccgatg tcggtatcag tgggatcatc attgacgtgt tgacgctgtc
      421 cagtaccttc ctgctggctt gcttcctggg gcagaaagtg tttggtctgg ataagcacac
      481 cagctggttg atcggtgccg gtagcagtat ctgtggtgct gccgcggtac tggcgactga
      541 gccggtagtg aaagcagaag ccagtaaagt aaccgtggct gttgcaaccg ttgttatctt
      601 cgggaccgtc gcgattttcc tctacccggc gatatatccg ctgatgtctc aatggtttag
      661 tccggaaact ttcggtatct acattggttc tactgtgcac gaagtggcgc aggtggtggc
      721 ggcaggtcat gccatcagcc cggatgcaga aaacgcagca gttatttcca aaatgctgcg
      781 cgtgatgatg ctggctccgt tcctcatcct gctggcggcg cgtgttaaac agctgtctgg
      841 ggcgaacagc ggcgagaaaa gcaaaatcac tattccgtgg tttgccatct tgttcatcgt
      901 cgttgccatc tttaactcgt tccacctgtt accgcagagc gtggtgaaca tgctggtaac
      961 gctggatacc ttcctgctgg caatggcgat ggcggcgctg ggtctgacca ctcacgtcag
     1021 cgcgctgaaa aaagccgggg cgaaacctct gctgatggca ctggtactgt ttgcctggct
     1081 gattgttggt ggtggtgcga ttaactatgt gattcaaagc gtcatcgcat aaaccactac
     1141 atcttgctcc tgttaacccg ctatcattac cgttttcctc cagcgggttt aacaggagtc
     1201 ctcgcatgaa atacattgga gcgcacgtta gtgctgctgg cggtctggca aatgccgcaa
     1261 ttcgcgccgc cgaaatcgac gcaaccgcgt ttgccttgtt caccaaaaac caacgtcagt
     1321 ggcgtgccgc accgctcacg acgcaaacca tcgatgaatt caaagccgcc tgtgaaaaat
     1381 atcactacac atcggcgcaa attcttcccc acgacagtta tctgattaac ctcggacatc
     1441 cggttgctga agctctggaa aaatcgcgcg atgcctttat agatgaaatg cagcgttgcg
     1501 aacagctggg gctttctttg ctcaacttcc accctggcag ccatctgatg cagatttcag
     1561 aagaggattg ccttgcgcgt attgccgaat ccatcaacat tgcgctggat aaaactcaag
     1621 gtgtcaccgc ggtgattgaa aacaccgccg gtcagggcag taacttaggg tttaaattcg
     1681 aacatctcgc ggcgattatc gacggcgtgg aagataaatc ccgcgtcggc gtctgcattg
     1741 atacctgcca tgctttcgct gccgggtatg atttgcgtac tcctgccgaa tgcgagaaaa
     1801 cgttcgctga ttttgcccgt attgtaggct ttaagtatct gcacgggatg cacctcaacg
     1861 atgcgaaaag cacctttggc agccgcgttg accgccatca tagcctcggc gaaggcaata
     1921 tcggtcatga tgcgttccgc tggatcatgc aagacgaccg tttcgacggc attccgctga
     1981 tccttgaaac catcaacccg gatatctggg cagaagagat cgcctggctg aaagcgcaac
     2041 aaactgaaaa agcggtggcc tgatcgatga acgacaggga aaagcagatt cttaaaattt
     2101 tacggcgtaa cccgctgatt caacagaatg aaatcgccga tattctgcaa atcagccgct
     2161 cacgtgttgc cgctcatatt atggatttga tgcgcaaggg tttgattaaa ggcaaaggat
     2221 atatccttac cgagcaggat tactgtgtgg ttgtgggtgc aatcaatatg gatattcgcg
     2281 gaatggcgga tatccgttac ccgcaagcag cctctcatcc aggttctgtt cactgttccg
     2341 caggcggcgt cggacgtaat atcgcgcata acctggctct gcttggtcgg gatgtgcatt
     2401 taatatcagc tattggcaac gatttttacg gcgagacatt actggaagaa acgcgccgcg
     2461 caggcgtgaa cgtttcaaac tgcattcgtc tgcatggaca cagcactgca acttatctcg
     2521 ctatcgctaa taagcaggaa gaaactatcc tggcaatcaa tgacacacat attctgcaac
     2581 agctaacgcc tcaactgctc aatacttcac gggacttgat tcgccatgct ggtgtggtgc
     2641 tggctgattg taatctcaca cctgaagccc tggagtgggt ctttaccatt gctgatgaaa
     2701 ttccgatgtt tgtcgatacc gtttccgaat ttaaagcgaa caaagtaaaa aactggtaca
     2761 gccggatcca cactctgaaa ccaacacaaa atgaactgga aattttgtgg gggcaaccga
     2821 ttaaagacga caacgatcgc atcagagctg taaattcgct gcatcaacaa ggtgttaaac
     2881 gcatttttgt ttatctgaaa gatgaatctg tattttgcag cgataaggat ggagaacaat
     2941 ttttgctgac cgcgccagcg catacgacag tagacagttt tggtgctgac gatggtttta
     3001 tggcgggcct ggtatatagc tttcttgaag gatacagttt ccgcgacagc gcccgttttg
     3061 cgatggcctg cgcggcaatt tcgcgcgcca gcggcagctt aaacaaccct accctgtctg
     3121 ccgataacgc gctttcatta gtgccaatgg tgtaacaatg ttgccggatt cgacgcttga
     3181 ccgcatctta tccggctcat aaattgcgca ctacgctaaa ccaataaaga atcctgcaat
     3241 agtagcactc atcaggttac tgacagcaaa aacgcaatag ccagtaatac caccattccc
     3301 agaacacttc tcatgacatc catagttatt atctcttcat gtcattttgg gcgacggctc
     3361 tctgcccatc ataaatagcc gggcctgcat cttgcgatac tgacccggct tgttatcaac
     3421 acttaatggg tttttatgta gccgcgtacg cactcttcga ctaatcccca gaaccagtcg
     3481 gtatcaatag tgatgccgac tttggtattg gcgggcttgc caagaacgcc cagctcgtcg
     3541 cagacggtac gcccatagca agggccactg ttaacgtcca cttcgacgta catctcctgg
     3601 gttttaatgc catcagggtt aatcaaataa ccgatgcagg tggcgtcgtg caccgggccg
     3661 ccagccaggc cgtagttttc gaactgcgtt ttgagagtga agttcatgat gtcgctgaac
     3721 agctctccgg cagggccgcc agccctttcc atccgagcaa tcacatccgg ggtgcaaacg
     3781 gtctggttgg taagatcgag gcccatcatc actaatggaa cgccggaggt gaacaccacg
     3841 cgtgcggcct ccgggtcagc aaagatgttg aactcagcag atggggtgaa gttgccagta
     3901 ccataagcac cgcccatcag aacaatttca cggattttcg gcaggatcgc gggttgcata
     3961 cgcattgcca ccgcgatgtt tgacaacgga ccaaccggca ccagagtgat atcgccatcg
     4021 cttgccataa gggtatcgat gatatatttc accgcatgag tgttttctgc ctggcgggtc
     4081 agcggctcga ataccgggcc atccagtccg gtttcaccgt gaatattatc ggcaacaatt
     4141 tgttgacgca taatgggctg cggcatcccc gcataaaccg gaacattaat ctccagtttc
     4201 tggcaaacat tcaggccatt aattaatgtt ttatcgaggg tttgattacc cgctacaatg
     4261 gtgacaccta ataaatcaat cgccggatgt ttcgccgcca tcattatagc aatagcatca
     4321 tcatgacccg gatcacaatc cagaataatt tttctctttt ccattgttta tttcctctgt
     4381 ttccagttgc gttattgttt ctacagcaaa gaaaattcga cctggccgat gattgaatct
     4441 taacaacagc gtacgtatgc taaatatgag aaatctcata gcggataaac atcgtgaaag
     4501 aaatccacaa taatgatctt aagcagcaat tgatgagtga atctgcgttt aaggattgct
     4561 tttcaacgga tgtttcagcc gatacgcggc tgtttcattt tttagcgcgt gactacattg
     4621 tgcaggaagg gcaacagccg tcctggctgt tttacctgac gcgaggccgc gccaggcttt
     4681 acgtcacgct tgctaatggt cgcgtgtcgc tgatcgattt ctttgccgcg ccctgtttta
     4741 ttggcgagat tgagttaatc gacaaagacc atgaaccgcg tgcagtgcag gctattgaag
     4801 agtgttggtg ccttgcgctc cctatgaagc attaccgccc gttgttatta aacgacacgc
     4861 tatttttacg aaaactctgc gtcaccttaa gtcataaaaa ttatcgtaat attgtttctt
     4921 taactcagaa tcaatcattt ccgttagtga atcgcctggc agcttttata ttactctcgc
     4981 aggaaggtga tctttatcac gaaaagcata cgcaagcggc agagtattta ggcgtttctt
     5041 atcgacatct tttatatgtt cttgcgcaat tcattaatga cggtttatta attaaaagca
     5101 agaaagggta tctcattaaa aacagaaagc agttgtcagg actggcgctg gagatggacc
     5161 cggagcataa attctccggg atgatgcagt aaaaattatt ggcaatagtg cgattgccgg
     5221 atgcaacgct taataaggcg tacatccggc aatgaaactc aagctaaacc aataaagaac
     5281 ccggcaatgg tcgcgctcat caggttggaa agcgtcgccg ccgccagcgc ccgtaaacca
     5341 agctgggcga tttccggcgc gcgttttggc gaaatagccg aaaacgctcc aacgacaaca
     5401 ccgatagaac caaagtttgc aaacccacag agggcgaacg agataatggc aatggtcttg
     5461 acatccagcg cgccgctcgt ttgcagatag ggcgagaaat tgagataagc aacaaactca
     5521 ttaatcgcca gtttctgccc aatcaaactt ccggcaagat ttgcatcact ccagtccacc
     5581 cccataatcc atgccaacgg ggccagcaca tagccgaaaa tgctttccag agagacattg
     5641 gcaaaaccaa accacccgcc aactccacca ataatgccgt tgatcagcgc aatgatcgcc
     5701 acaaatgcca ttaccaccgt cgccacaccg gcggcgattt tcagcccggt cattgcaccg
     5761 ttcgccgcag cttcaataat gctttttggc ggtgtttcgc taaacgacag attttcaaac
     5821 gtaacttgcg aaggttccgt cgccggacta agaatacgtg caaacaggat accgccagga
     5881 atagccatca gcgaagcggc taacaaatag tcaatcggca cgcccattcc ggcatagcca
     5941 atcatcatcg aacccgcaat agacgccatg ccgctacaaa tagccgtaaa tagctcgttg
     6001 cgattcatgc gatcaataaa aggtttaacg atcgccggga tctcattttg cccgaggaaa
     6061 atagtggtga ctgcgacaaa agattcgatt ttgctgatgt tgagggcttt ctgaaaaata
     6121 ctgccaagaa tacgaatcag cagccccatt acgccaatgt agtacagcaa gctgataagc
     6181 gcggtaacga aaataatcgc cgggagtacg cgaaaggcga agataaagcc cgcaccgtca
     6241 aacagaacat ccattttggg accgaccagc gaaccaaaaa taaaggcact tcctgcgtca
     6301 ctgtaggaca tcactttatg gacgcctaat gccgcctgct ctactgccca ttttcccggg
     6361 ggaaaataga gcatgatgcc accaatggca atttgcagca gcagcgcagc cccaaccgtg
     6421 cgcaagctga tgctcttttt atttaccgac aacagaaatg ctattgccag taacaccacc
     6481 atccccacaa cacttctcat tatatccata atgattttcc cttcatgcca gtaaacccgg
     6541 cgtcagcgcc aggttttggt atgtttgatg agtacgggcg acggctttct gcccaccaga
     6601 aattaaccca cgagacgctg atattctttg gcaatttcgc tcgccagaat ggcgttatta
     6661 aagaccagtt gaatattgga tttcaggcta tcaccgccgg ttagctccgc aacacgcgcc
     6721 agcaggaacg gtgtactctc tttcccaata acaccctgtt cttcggcttc agcaacggct
     6781 tgatcaattg ctgcattgat ggtttcttct ggcatcgcaa actgttccgg gatcgggttc
     6841 gctaccacca ggccgccgtt caaaccggtt tgccacttca ctgccatcgc gcgagcaatt
     6901 tcgcgggcac tgtcaagacg aatgctgact tcaaatgggc tggttcggca gaaaaacgca
     6961 ggcagcgctt ttgtctgata accaattaac ggtacaccga aggtttctaa atattctgtt
     7021 gttaatccta aatcgagaat agattttgcc cccgcacaaa caacagtgac attggtattt
     7081 gccagttcct gtaaatcggc agaaatatcg aaggtatgtt ccgctccgcg atgcacacca
     7141 ccaatacctc cggttgcaaa aactttaatc ccagccagtg ctgcaataat catggttgat
     7201 gcaacggtag tcgcgccatt tttccctgca gcaacgacaa aaggtaaatc acgacgacta
     7261 actttcgtca cgttatgtcc ttcacggccc agcaattcga tttcttcttt acttaagccc
     7321 actttcatca caccgccaat aatggcgata gtggctggta ctgcgccctg tttacgaatt
     7381 gtttcttcta cttcaattgc ggtttgggca ttttgtggga atggcattcc gtgagaaatt
     7441 atggtcgatt ccagcgcaac aaccggtttt ttatttttta aagcatcctg tacttcagcg
     7501 gatatttgta ataattcagg ggacagagtt aattcagaca ttctgtgttc tccactaacg
     7561 atgtaacatt ggcaattgat aattcagggt tgttggtata ttcacaggca agtgccattg
     7621 acgaacatcc ctgcgcaaat cgaacagaat cgataaacgg cataccgtct acccaacagg
     7681 aggccagccc cgccatcatg gcgtcgcctg ccccagtaac attgatgaca ttcgttttaa
     7741 taggggccga ccacccactt tcaccgttga tatcactgta gtaaacgcca tcccctccca
     7801 tgctaagcac caggcggttc aggccgtgtt gatgaaacca ggcggcgacc tttgcgacat
     7861 cttcacgtcc ggaaagtgca attccgctca acgtttcggc ttcaagacgg ttaggtttta
     7921 acgtatggat tttgctcaga tgatcgcgga ttttgacgca tttccatgcc gataccggat
     7981 caataaatac aggcgtttcc ccgctatttt ccagaaccca gaccagcgcc tcttcactaa
     8041 ggttgcaatc cgcgacaata acctttgcac cgcgaataaa ctcctgatgc tgggcaagaa
     8101 attctgcaga aatacagtca ctaatactca tatcatttat cgcgacaagc atttctcctg
     8161 tattatcgag cagtgataaa tagctggatg tattctcacc cggaacaatt aagcatttat
     8221 cgacataaac accggattga ttagtttgcg ccagtaatga ttgcccataa aaatcaccac
     8281 ctactgcact taaaagccag gcatttttcc ccagtaacgc cagattatga gcgatattcc
     8341 ttcccacgcc acctggggta aatttaattt ttccgggatt cgaatccgca taattcaatg
     8401 gatgatgtga atacccagcc acatcaatat tcgccgagcc aataattact atgtagtcct
     8461 tttcgcgcat agcacgcccc ctggcaataa attgccaacg aagaaattgg gtaaaatagt
     8521 cattacagtc aataagacta ttagagcacg tgttcagatt taaacacatg ttcataataa
     8581 gcatgtttaa cgcaatgtaa atcgatcaga aaaaacgaaa tcggccagat atgaaggcgg
     8641 tcacaaaatt tgtctattta tgcagcaaca aaagagaaag ttgtgatgtg taacacacaa
     8701 tatggcaggc aagaaaaagg gcagagatgt ttctgccctc agattgttga caatgcgcgc
     8761 tttgttcatg ctggatgtgg cgtaaacgcc ttatccgacc tacaaaagcg caaaaattca
     8821 atatattgca gggttcatgt aggtctgata agcatagcgc atcaggcaat tttgcgtttg
     8881 tcatcatcgg ggcagagatg tctcagcccc gatgatgaag agaaattatg cgtttttttc
     8941 tactatttgt gtttccgggc gtttgaggaa ggcataggcc aaacccgcca ccagcgtacc
     9001 ggcaataatt gctaccaggt aacccaatac cggcgtaata gcgccaggga tcagcagaac
     9061 aaacagacca ccgtgtggtg ccatcagttt tgcaccaatc gccattgaga ttgcgccagt
     9121 cagcgcccca ccaacgatac agcacggtag cacacgcatc ggatcacgag cagcaaacgg
     9181 aattgcacct tccgaaataa agcacagacc aagtaccaga gcggctttgc caccttcctg
     9241 ctgcgctttg tcgaatttgc gacgcgccac cattgttgcc agacccattg ccagcggtgg
     9301 caccatacct gccgccataa tcgccgccat cgggccatag gtttgagtac tcagcagacc
     9361 cacaccgaat gcgtacgctg ctttgtttac cggaccgccc atgtcagtac acatcatgcc
     9421 accgaggatc gcccccagca gaaccgcatt cgcagtcccc atggtctgca gccagtgagt
     9481 caacccatcg agaatgccag caaccggttt accgattagg tagatcatcg ccagaccgac
     9541 caccagactg gaaattagcg gaatgatcag gatcggtttc agtgcctcca tactctgtgg
     9601 cagtttcagt tgcgtactga ttaacttcgc aatgtaaccc gccaagaagc ccgcaataat
     9661 accaccaatg aagccagaac cggtgctaac cgccagcata ccgccaatca gacccggcgt
     9721 gagacccgga cgatcggcaa tggagaaggc aatataacct gccagtaccg gcaccatcag
     9781 cgcaaaggct gaaccaccac caatctgcat cagcgccgct gccaacgtac ccggctcttt
     9841 aaacgcttcg ataccaaaag caaaagaaag cgcgatacac agaccacctg caacaaccat
     9901 cggcagcatg taagagacgc ccgtcagcaa gtgacggtaa gcgcctgcac tctctttctt
     9961 accttcagtg gtcgctgttt gagttttgcc cgccggttca tacggcgttg cttcagcaac
    10021 cgctttatcc agttcctgcg cagttttctt cagcgccaga ccggtagagg tgcgatacat
    10081 cggtttacca gcaaatttcg ccagatctac ttcgatatct gccgccacaa tcaccagatc
    10141 cgctgctgcg acttcttcag gggtgattgc attacccgcg ccaacagaac cacgggtttc
    10201 aactttcacc caccagccac gttttttcgc ttcggtttca atggcttcag ccgccataaa
    10261 ggtgtgtgct acgccagtcg ggcaagcagt caccgcaact acgcgtttcg gaccgctggc
    10321 agcaaccggt gctgtcgcag cgaccggcgc agtgtaaggt ttcgcatggc ctttggcttc
    10381 actcaggaac agctcagggt gcgcaactgc ccgggaaata tcgcccagcc agacattttt
    10441 accgttcagc gcgctgtcgt tcgggatgga atcaccgaga acaatcgcca tttcagcgtc
    10501 gttcggattg tcgatgattt ccagttttgc ttttcgcgcc gccgcgccca gcagggtctt
    10561 cgccatatag gcgcgtgcct gaccgagatt agcgtcaata atcagcagcg ttttcattat
    10621 gcctctcctg ctgtcagtta aaaggttgta agtcgacgcg cgccatcatt gcggccaact
    10681 gcggacgatc ggtaataccc acattgcttt gacttaccgc cagggctgca acagctgtcg
    10741 ccagacgcag tgtgtgttca ctggattcac gcatcagcaa gccataaatc aggccaccaa
    10801 ccatagaatc ccctgcgcca acggtgctta cgacatcgac tgacggtggt ttggcgatcc
    10861 attcgccgga ggcattaacc caaagcgcgc cttcggcacc cagtgaaata acaacatgcg
    10921 caataccttg ttcacgtagc gcatgtgcag cttcaatcac atctttcatt tcaggcagtt
    10981 tacggcctgc ccagatttcc aactcgcggc ggttaggttt caccagccac ggtgccgctt
    11041 tcaaacctgc tactaacgct tcacggctac tatcaaagat aatgcaagga cactgactac
    11101 gcaggcgagt catccagtcg gtgaacgctt ccgggctgac gcctgacggt aagcttccgc
    11161 tgacacagac catatcgaac tgaccgagcc agctcagaga atcagtcaca aagcgttccc
    11221 agtcggcggg ggtgacttca aaacccgaga agttgaagtc ggtcacttcg ccgtcttttt
    11281 ccgtcagctt aacgttaatt cgggtgcgcc cctgtacaac ctggaaacgg ttggcaatgc
    11341 ccagctcgct gaacagttgc tgaaaaccat cctgattgtc tttacccagg aagccgccaa
    11401 cggtgacatc aattcccagg tcttttaata ctttggctac gttgatgcct ttacccgccg
    11461 catgcagacc agtggttttc accaggttca cttcgccgcg ttcaatttcc gggcagaaac
    11521 caacaaggtc ataagccgga ttaagggtga tcgtagcaac acgtctgctc attatgcgcc
    11581 ctccccaaga ccagcagcga tagcgtcgcc aatggctttc agcgcctgtt cagcatctgc
    11641 accctgggcg gtaaagcgta ggcgatgacc tttcttaacg ccaagtgcca caactttcat
    11701 cagactacgt ccgtttgccg gtttgccggt gccatcaagg tttgtcacgg taatatcact
    11761 gttaaattgt ttaatggtat tgaccagcat ggtacctgga cgagcatgca ggccgtgttc
    11821 attgcgcacc acaaactccg cgcttaacac gtcgtcggtc ggcgcatcat cgctggtcag
    11881 cagcgccagc aacgttgccg catccgcttt cagcaagcgg tcagctttat tatcgagcaa
    11941 caaatcagcg agacgcttaa gaaccgcgat gggctgatcg tcattcatcg ccacactcac
    12001 cagcatggct gccgtttcgc cgtccacatc aaaagcattt gccgcacggc ttaccgcaat
    12061 cgcgctacgc agattgcctt cggcgctatc gctcagccag ataccctgcc cgaggttcag
    12121 cggttgttca ttgatggctt tggtgacgaa agtggcgtca actgcccccg cttctttcag
    12181 acgcgcagcg ttcagcgcct gaagagtcag cagatcgctg gcgacgatat ccagcgtcag
    12241 catttcgttg tcgagcttca gctgctcact ctgcttttcg cccatcagca atgcgcgaag
    12301 ttcttctgct gttgttgctg acttcagttg ttcagcaacg gaatcatcgc tcagtacgtg
    12361 ggtcagctgg cgtagcaggc ccagatgttc atccgagctg gcagcaatac cgatcgccac
    12421 gtacgctacc tggccgtcac cccaggtgac gccttccggg aactgaaata cctgaacgcc
    12481 ggttttcagc acctgatcgc gggtgtcggt agtgccgtgt ggaatagcaa taccattgcc
    12541 gaggaacgtt gaggtttgct gttcgcgcgc cagcatgcca ttgacgtagc cttcagctac
    12601 attaccggcc tgcaccagcg ccgcagcgac ctggcgaatc gcctcttctt tgtctccggc
    12661 cttttcgccc ggatggatgt cctgtacgga taactggaac atagttctcc tctcttgctg
    12721 aattgaaacg attcagcttc tatgagaaaa aaagcgccaa cctggcttag ggttaaagac
    12781 aagatcgcgc tgaaacgttt caagaaagca taatactttt gtttcagcac gcaaggaaag
    12841 ccgtaaagtt atgagcaaaa gtttgatgtg ctgcacaatt ttttgtcatt tttcctaatt
    12901 gctgatggga aaatccgtta tcagcaattt catttcagca gcgtcagcag ccaatcagca
    12961 acagtgaaat ggcattttga ttattcgtgg cgcttttgat ttcgctgtct gtttattttc
    13021 tgacaagcag cgtaaactcc gcgtcttcct cttccagtga tcgaccagca tgcataactc
    13081 ccccgcagtc accagcgcga aatcgtttga cctgacctcg atggcgtttt taatcgttgc
    13141 ctttctcacc ggtattgcgg gcgctctgca aaccccgaca ctcagtattt ttcttactga
    13201 tgaagtacat gcccgtccgg cgatggtggg attcttcttt accggcagcg ctgtcattgg
    13261 gattctggtg agtcagtttc tcgccgggcg ctctgataag cgcggcgatc gcaaatcgct
    13321 gattgtcttt tgctgcctgt taggcgtgct ggcctgcacc ctttttgcct ggaatcgcaa
    13381 ctactttgtt ttgctattcg ttggcgtctt tcttagcagc tttggctcaa ccgctaaccc
    13441 gcaaatgttt gcccttgccc gtgaacatgc tgacaaaacc ggacgtgagg cggtgatgtt
    13501 cagctctttt ttacgcgctc aggtttcact ggcatgggtc attggcccac cgctggctta
    13561 tgccttagcg atgggtttca gctttacggt aatgtatctg agcgcagcgg tagcgtttat
    13621 tgtttgcggc gtgatggtgt ggctgttttt gccgtcgatg caaaaagagc ttccgctggc
    13681 gaccggcacg gttgaagcgc cgcgccgtaa ccgtcgcgat acgttgctgc tgtttgtcat
    13741 ttgtacattg atgtggggct cgaacagcct gtacatcatc aacatgccgc tatttattat
    13801 caacgaactg catcttcccg agaaactggc cggtgtgatg atggggaccg ccgcagggct
    13861 ggaaatcccg accatgttga ttgccggata ttttgccaaa cgtctgggta agcgcttctt
    13921 aatgcgcgtt gctgccgtgg gtggcgtctg tttttacgca ggaatgctga tggcgcattc
    13981 acctgtcatt ctgttgggct tgcagctgct aaatgctatt tttattggca ttctgggcgg
    14041 tatcgggatg ctctattttc aggatttgat gcccggtcag gcaggttcag ccaccacgct
    14101 ctataccaac acgtcgcgcg tgggctggat catcgcagga tcagtggcgg gcatcgtcgc
    14161 cgagatctgg aattatcacg ctgtgttctg gtttgcgatg gtgatgatta tcgccactct
    14221 gttttgctta ctgcgaatta aagatgttta aggcgcggtc aacatttcca gatcgataag
    14281 ccaggtcatt gcctgctgac gcgagttacc gcacatttca gcgctggctt gtaagcctgc
    14341 acagactttc ggtcgcagtg gcgaggtgaa aattttacag cgttgctgtt catcaagctg
    14401 aatgcagggc gtattggcgg gcttgccatc tggcataccg ggaatagggc tggaaattga
    14461 aggggcagtg caacacgccc cgcaacccgg acggcattcc atacgttgtt ctcttaaggt
    14521 taaccaccgg attgatgagc gcgcacagta ccaccttttt tgcaccagca aaagtgcgaa
    14581 taccacttgc ccgaaaggcc cgtcgcgagt actttgtcgc gatatttttg acattttcga
    14641 ctacaggaat ttttcgatgc caagagcgaa cgaaattaaa aaaggtatgg tactgaatta
    14701 caacggcaaa ctgctgctgg ttaaggacat tgatattcag tcgcccactg cccgcggtgc
    14761 cgctacgctg tacaaaatgc gtttttctga tgtccgtacc gggctgaaag tagaagagcg
    14821 tttcaaaggt gatgatatcg ttgacaccgt gacgctgacc cgccgttacg ttgacttctc
    14881 ctatgtcgat ggcaacgaat atgtctttat ggataaagaa gactataccc cgtatacctt
    14941 caccaaagat cagattgaag aagagttgct gtttatgcct gagggcggca tgccggacat
    15001 gcaggtgctg acctgggacg gtcaattgct ggcgcttgag ctgccgcaga ccgttgatct
    15061 ggaaatcgtt gaaactgcac caggcatcaa aggggcatct gccagcgccc gtaacaaacc
    15121 ggcgacattg agcactggtc tggtgattca ggtaccagaa tacttaagcc cgggcgaaaa
    15181 aattcgtatc catatcgaag aacgccgtta tatgggtcgt gctgactaac ttcagccgca
    15241 tgcagaaaag ggatagctca ggctgtccct tttttaattt attataccaa tcttctattt
    15301 tgcgcttcct gaacacctta cgcccgcaat tttcactcgt tgattgatcg ccctcacact
    15361 tcatcgcatt aacaatccag accaatttca attgttgtca tataacttta cactgtcatt
    15421 gttaactaat cgttactaag acgtgactcc tatgaataca atcgcctccg ttacgctccc
    15481 gcatcatgta cacgctccac gctacgatcg acagcagttg caatcacgta tcgttcattt
    15541 tggctttgga gcctttcacc gcgcacatca ggcattactg accgatcgtg tgctgaatgc
    15601 ccagggcggc gactggggga tctgtgaaat cagcttgttc agcggcgatc aactgatgag
    15661 ccagctccgc gcacagaacc atttatatac cgtgctggag aaaggtgcgg acggcaatca
    15721 ggcgataatt gtcggtgccg ttcacgaatg tctgaatgca aaactggatt ccttagcggc
    15781 aattattgag aaattttgcg agccacaggt ggcaattgtt tccctgacga ttaccgaaaa
    15841 aggctattgt attgacccgg ccaccggtgc actcgacacc agcaatccgc gtattattca
    15901 cgatctacaa aaccctgaag aacctcactc cgcgccaggt attctcgtcg aagcactgaa
    15961 acgccgccgt gagcgcggcc ttacaccgtt taccgtgctc tcctgcgaca atattcccaa
    16021 caatggccat gtggtgaaaa acgcggtgct gggaatggca gaaaaacgtt cgccagaact
    16081 cgccgggtgg ataaaagaac acgtcagttt tccgggaacc atggtcgacc gcattgttcc
    16141 ggctgcaacc aacgaatcac tggcggaaat cagccagcat ctgggggtga atgatccctg
    16201 cgcgattagc tgcgaaccgt ttatccagtg ggtggttgaa gataacttcg tcgctgggcg
    16261 tcctgcctgg gaagtcgcag gtgtacaaat ggtgaatgat gtcctgccat gggaagagat
    16321 gaaactgcgg atgcttaatg gcagccactc ttttctcgct tatctgggtt acctctcagg
    16381 attcgcccat atcagtgatt gcatgcagga tcgcgcattt cgccatgccg ccagaacatt
    16441 aatgctggat gagcaagcgc cgacactgcg gattaaagat gtcgatttaa cacaatatgc
    16501 ggataagtta attgcacgtt ttgctaatcc ggcactgaaa cataagacct ggcaaatcgc
    16561 gatggatggc agccaaaaat taccgcaacg catgctggca ggtattcgca tacatctgga
    16621 gcgcgaaacg gactggtcgt tgctggcatt aggcgttgca ggctggatgc gttacgtcag
    16681 cggcgttgat gatgccggaa atgccattga tgttcgcgat ccgcttagcg ataaaattcg
    16741 tgaacttgtt gcggtcagca gcagtgaaca acgcgtaacc gccctgcttt ccctgcgtga
    16801 aatttttggt gatgatctgc cagataaccc gcattttgtg caggccatcg aacaagcctg
    16861 gcaacaaatc gcacaattcg gcgcgcatca ggcgctatta aacaccctca aaatttaacg
    16921 atttctgcgg ttaaagcgga tgaagctcgc cttcgtccgc tctccccttc tcttttctgc
    16981 cttttttagc caggattaac gctcagttaa cttaccagag tattgcggag ccgttgtgac
    17041 cagaaccaac ctcatcaccg gttttctcgg cagcgggaaa actacgtcga ttcttcatct
    17101 gttagcccat aaagatccca acgaaaaatg ggcggtactg gttaatgaat ttggggaagt
    17161 cggaattgat ggtgctttgc tcgccgatag cggcgcactg ttgaaagaga tccccggcgg
    17221 ctgcatgtgc tgcgttaatg gtttacccat gcaggtaggg ttgaatacct tactgcgtca
    17281 gggaaaacca gaccgcttgt tgatagagcc gaccgggctg ggccatccga aacagatcct
    17341 cgatctctta accgcacccg tctatgaacc gtggatagat ctgcgcgcca cgttgtgcat
    17401 tctcgatcca cgcctgctgc tggacgaaaa aagcgccagc aatgaaaact tccgtgacca
    17461 gctggctgcc gcagacatta ttgtcgccaa taaatccgac cgtgcgacgc ccgaaagtga
    17521 gcaagcgcta gagcgctggt ggcagcaaaa tggtggcgat cggcaattaa ttcacagcga
    17581 gcatggaaaa attgacggtc atcttctgga tttgccgcgt cgcaatttag ccgagttgcc
    17641 cgccagcgcc gcgcattctc atcagcatag cgtgaaaaaa gggttagcag cgttaagcct
    17701 gccagagcat caacgctggc gtcgcagtct gaacagcgga caagggcatc aggcctgtgg
    17761 ctggatattc gacgctgata cggtattcga caccattggt attctggaat gggcgcgact
    17821 tgcaccggtg gaacgcgtca aaggcgtgct gcgtattccc gaagggctgg tgcgaatcaa
    17881 ccgtcagggc gatgacctgc acattgaaac gcaaaacgtt gcgccaccgg acagtcgtat
    17941 tgagctgatt tccagcagcg aagctgactg gaatgccttg cagagcgcgc tgttgaagct
    18001 tcgtttagcg actaccgcgt aaggttgcct gcgtttttca gtaagataat tagagaaaat
    18061 atgattaaaa atttgccgca aatagtgttg ttgaatattg tcggcctcgc gctgtttctt
    18121 tcctggtata ttcccattaa tcatggattc tggttgccga ttgatgcgga tattttttat
    18181 ttctttaatc agaaactggt cgaaagtaag gcctttttgt ggctggttgc attgaccaac
    18241 aatcgcgctt tcgacggttg ttcactgctg gcgatgggaa tgttgatgct gagtttctgg
    18301 ctgaaagaaa acgccccagg cagacgacgt atcgtgatta ttggtctggt catgctatta
    18361 actgcagtgg tattaaacca gctgggtcag gcactaattc ctgtaaaacg ggccagccca
    18421 acgttgactt ttaccaatat taaccgcgtc agcgaactgc tctctgttcc cacgaaagat
    18481 gcctcacgag atagctttcc cggcgatcac ggtatgatgc tgcttatttt ttcggcattc
    18541 atgtggcgtt atttcggcaa agttgcaggc cttatcgccc ttattatttt tgtgattttt
    18601 gcgtttccca gagtaatgat tggcgcacac tggtttactg acatcatcgt cggttccatg
    18661 accgtgatat tgatcggctt gccctgggtg ttgctgacgc cattaagtga tcgattaatc
    18721 accttttttg acaaatcact acctggaaaa aacaaacatt tccaaaacaa ataactcaca
    18781 gtaattaaca tcatcaggtt tatttttata gtgaggataa tcctgatgat gcgcctcctg
    18841 ctttcatcca tcgagcgcaa aaatcattct ctaagtaaat gaatggattg catgcgtttc
    18901 actcaatcgt actttaattg accaatcccg cttattaact ttctgtatca ctttttctta
    18961 taaaaaatca tgtaaaaccg ctcgccaaga ccgcaccaat cgggtaatct cgaactcgtt
    19021 ttgcctcggc ggtagattat cctcacagca tataattttg tgcgttagtc cacagatttg
    19081 gccttaagga attgtttcaa catgcccagg taattagtct cgtgtcgctt ggcatttttt
    19141 tataacgata tttgtcgtta aggacttcaa gggaaaacaa acaacatggt caaatctcaa
    19201 ccgattttga gatatatctt gcgcgggatc cccgcgattg cagtagcggt tctgctttct
    19261 gcatgtagtg caaataacac cgcaaagaat atgcatcctg agacacgtgc agtgggtagt
    19321 gaaacatcat cactgcaagc ttctcaggat gaatttgaaa acctggttcg taatgtcgac
    19381 gtaaaatcgc gaattatgga tcagtatgct gactggaaag gcgtacgcta tcgtctgggc
    19441 ggcagcacta aaaaaggtat cgattgttct ggtttcgtac agcgtacatt ccgtgagcaa
    19501 tttggcttag aacttccgcg ttcgacttat gaacagcagg aaatgggtaa atctgtttcc
    19561 cgcagtaatt tgcgcacggg tgatttagtt ctgttccgtg ccggttcaac ggggcgccat
    19621 gtcggtattt atatcggcaa caaccagttt gtccatgctt ccaccagcag tggtgttatt
    19681 atttccagca tgaatgaacc atactggaag aagcgttaca acgaagcacg ccgggttctc
    19741 agccgcagct aataaaccgt ttggatgcaa tcccttggct atcctgacga gttaactgaa
    19801 agcactgctt aggcagtgct tttttgtttt cattcatcag agaaaatgat gtttccgcgt
    19861 cttgatccag gctatagtcc ggtcattgtt atcttttaaa tgttgtcgta atttcaggaa
    19921 attaacggaa tcatgtttat acgcgctccc aattctggac gtaagctcct gcttacctgc
    19981 attgttgcag gcgtgatgat tgcgatactg gtgagctgcc ttcagttttt agtggcctgg
    20041 cataagcacg aagtcaaata cgacacactg attaccgacg tacaaaagta tctcgatacc
    20101 tattttgccg accttaaatc cactactgac cggctccagc cgctgacctt agatacctgc
    20161 cagcaggcta accccgaact gaccgcccgc gcggcgttta gcatgaatgt tcgtacgttt
    20221 gtgctggtaa aagataaaaa aacattctgt tcatctgcaa ctggtgagat ggacattcca
    20281 ctcaatgaat tgattccggc gctcgacatt aataaaaacg tcgatatggc gatcttaccc
    20341 ggcacgccga tggtgccgaa caaacccgca atcgtcatct ggtatcgcaa ccctttgctg
    20401 aaaaatagcg gcgtgtttgc cgctctgaat ctcaacctga cgccttcact cttttatagt
    20461 tcacggcagg aagattacga tggcctcgcc ctcattattg gtaatactgc gctatctacc
    20521 ttttcttcac gtttgatgaa cgttaatgaa ttaaccgaca tgccggtccg tgaaactaaa
    20581 attgcgggca ttcctctgac cgttcggctt tatgcagatg actggacatg gaacgatgtg
    20641 tggtacgcat ttttactggg tggcatgagt ggaactttcg ttggacttct ctgctattac
    20701 ctgatgagtg tgcgtatgcg cccaggcaga gaaatcatga ccgccatcaa gcgcgaacaa
    20761 ttttacgtgg tgtatcaacc ggtggttgat acacaagctt tgcgggtaac gggcctggaa
    20821 gtactgctac gctggcggca tccagtagca ggtgaaatcc ccccggatgc cttcattaac
    20881 tttgccgaag cgcaaaagat gattgtgcca ctgactcagc acctgtttga gttgattgcc
    20941 cgcgatgccg cagaattaga aaaagtactg ccggtaggcg tcaaatttgg cattaacatt
    21001 gcgccggccc acttgcacag cgaaagcttt aaagcggata tccagaaact gctcacttcc
    21061 ctgcccgcac accatttcca gattgtgctg gaaattaccg agcgcgatat gctgaaagag
    21121 cgagaagcca cacaactctt cgcctggctg cactcggtcg gcgtagaaat tgctattgat
    21181 gacttcggta ccgggcacag cgcgcttatc tatcttgagc gttttacgct cgattatctg
    21241 aaaattgatc gtggatttat caacgccatc ggtacggaaa cgatcacttc acccgtactt
    21301 gacgcggtgc tgacgctggc gaaacgtctc aatatgctga cagttgctga aggggtcgaa
    21361 acgccagaac aggcacgatg gctaagcgaa cgcggcgtta atttcatgca aggctactgg
    21421 attagtcgcc cgttaccgct ggacgatttt gttcgctggc tgaagaaacc gtatacgccg
    21481 cagtggtaag gtgtgcttac gtcccttatt attcatagtg aaagcatgcc ggattgcggc
    21541 taatgatgag taaaaggaaa tccgttgcag atgattgtgc gcatactgct gctgtttatc
    21601 gctctgttca cctttggtgc gcaggcgcag gctatcaagg aaagctatgc ctttgccgta
    21661 ctgggcgaac cccggtacgc atttaatttc aaccattttg attatgtgaa ccccgccgcg
    21721 ccaaaaggtg ggcaaataac gttgtctgcc ctcggcacct tcgataattt caaccgctat
    21781 gcactgcgcg gcaacccggg cgcacgcacc gagcagctgt acgacacgct atttacgact
    21841 tccgatgacg aaccaggcag ttattacccg ctgattgctg aaagcgcacg ctatgctgac
    21901 gattattcct gggtggaggt cgctattaat ccacgcgccc gttttcatga tggttcgccc
    21961 attactgccc gcgatgtaga gtttactttt caaaaattta tgaccgaagg cgtgccgcaa
    22021 tttcgtctgg tctacaaagg caccaccgtc aaagccattg cgccgttaac cgtgcgcatt
    22081 gagttagcta aacccggcaa agaagatatg ctgagtctgt tttcgctgcc ggtatttcca
    22141 gaaaagtact ggaaagatca caaacttagc gacccgctcg ccacgcctcc gcttgccagt
    22201 ggtccgtacc gcattacgtc ctggaaaatg gggcaaaata ttgtctattc ccgcgtgaaa
    22261 gattactggg cagcaaactt accggtaaac cgtggacgct ggaatttcga caccattcgc
    22321 tacgattatt acctcgatga taatgtcgcc tttgaagcgt ttaaagcagg agcctttgat
    22381 ttgcgtatgg aaaacgacgc caaaaactgg gccacgcgct ataccggtaa aaatttcgat
    22441 aaaaaataca tcatcaaaga tgagcaaaag aacgaatcag cccaggatac gcgctggctg
    22501 gcgtttaata tccaacgtcc ggtattcagc gatcgccggg tccgggaagc gatcactctc
    22561 gcctttgact ttgaatggat gaacaaagcg ttgttttaca atgcctggag tcgcacgaac
    22621 agttattttc agaataccga atatgcggcc agaaattacc ccgacgccgc ggagctggtg
    22681 cttctggcac caatgaaaaa agatctaccg ccagaagtct tcacgcaaat ctaccagccg
    22741 ccggtatcca aaggcgatgg ctacgatcgt gacaacctgt taaaagccga caaacttctc
    22801 aacgaagcag gctgggtgtt gaagggtcag caacgcgtta atgtcacaac gggtcagcca
    22861 ctcagctttg aattattgct tcccgcaagc agcaatagtc agtgggtatt gccgttccag
    22921 cacagcctgc aacggctggg tatcaacatg gacattcgca aggtggataa ctcgcaaatc
    22981 accaaccgca tgcgcagtcg cgactatgac atgatgccgc gcgtatggcg ggcgatgccg
    23041 tggcccagtt ccgatttaca gatttcctgg tcatcggaat atatcaattc cacttataat
    23101 gcccccggcg tgcaaagccc ggttatcgac tcgctgatca accaaattat tgccgcgcag
    23161 ggaaataaag aaaaattact gccgttgggg cgagcactgg atcgcgtatt aacgtggaat
    23221 tattacatgc tgccaatgtg gtacatggcg gaagaccgtc tcgcctggtg ggataaattc
    23281 tcccaacccg ctgtacgccc tgtttacagc ctgggtatcg atacctggtg gtatgacgtt
    23341 aataaagcgg ccaaactgcc gtcagccaga caacagggag agtagatggg cgcttatctg
    23401 atccgccgtc tgttgctggt gatcccaacg ttatgggcga ttatcactat caactttttc
    23461 atcgtgcaaa ttgcgcctgg cggtccggtc gaccaggcca tcgccgccat tgagtttggt
    23521 aatgccggag tattacccgg cgcaggcggt gaaggtgttc gtgccagcca tgcgcaaacg
    23581 ggtgtcggca atatcagcga cagtaattac cgtggcggac gcggattaga tccagaagtg
    23641 atcgctgaga tcactcatcg ctacggtttc gataagccga tccacgaacg ttacttcaaa
    23701 atgctctggg actacatccg ttttgatttt ggcgacagcc tttttcgcag cgcctcggtg
    23761 ctgacgctga ttaaagacag tctgccggtc tccatcaccc tcggattgtg gagcacgctg
    23821 attatctatc tggtgtcgat tccgttaggc attcgcaaag ctgtttataa tgggagccgt
    23881 tttgacgtct ggagtagtgc atttatcatc atcggctacg ccattccggc ctttttgttt
    23941 gccatcctgc tgattgtctt cttcgcaggc ggcagctatt tcgacctgtt ccctctgcgc
    24001 ggcctggttt ccgctaactt tgattcgctg ccgtggtatc agaaaatcac cgattatctg
    24061 tggcatatca cgctgccggt gctggcgaca gtgattggcg gctttgcggc tctgaccatg
    24121 ctgacaaaaa actcattcct tgatgaagtg cgtaagcaat acgtggtgac ggcacgtgcg
    24181 aaaggggtaa gtgaaaaaaa tattctctgg aaacatgtgt tccgcaacgc catgctgctg
    24241 gtgattgccg gttttccggc gacgtttatc agcatgtttt ttaccggctc gctgctgatt
    24301 gaggtgatgt tttcactcaa tggtcttggc ttactgggct acgaagcgac cgtctcgcgc
    24361 gattatcctg taatgtttgg taccttgtat attttcaccc tgattggcct gctgctgaat
    24421 attgtcagtg atatcagcta tacgctggtc gatccgcgta tagactttga gggacgttaa
    24481 tgtcgcgact cagccccgtc aatcaggccc gttgggcgcg ttttcgccat aaccgtcgcg
    24541 gctactggtc gttatggatt ttcctcgtct tgtttggttt gagtttgtgt tctgaactta
    24601 tcgccaacga taaaccgttg ctggtgcgtt atgacggcag ttggtatttc ccgttgttga
    24661 aaaactacag cgaaagcgat tttggaggcc cgctggcaag tcaggctgat tatcaggacc
    24721 cgtggctgaa acaacggctg gaaaataacg gctgggtact gtgggcaccg attcgctttg
    24781 gtgctaccag tatcaacttt gctaccgata agcccttccc ttctccaccc tcccggcaaa
    24841 actggctggg aacggatgcc aatggcggcg atgtgctggc gcgcattctc tatggcacgc
    24901 ggatctcagt tctgtttggc ctgatgctga ccctctgctc cagcgtgatg ggcgtgctgg
    24961 cgggggcgct acaaggctat tacggtggta aagtcgatct ctgggggcaa cgttttattg
    25021 aagtatggtc ggggatgccg acgctgtttt tgattatttt actttccagc gtcgtacagc
    25081 ctaacttctg gtggctgttg gcaattaccg tcttgtttgg ctggatgagt ctggtcggtg
    25141 tggtgcgggc ggagttttta cgtacccgta atttcgacta catccgcgcg gcgcaggcgc
    25201 ttggcgtcag cgatcgcagt atcatcctgc gtcatatgtt gccaaatgcc atggtcgcta
    25261 ccctcacttt tttaccgttt attttatgta gttcgataac caccctgacc tcactcgatt
    25321 tcctcggctt cggtctgccg ctcggttcac cgtcactcgg tgaactgctg ttacaaggga
    25381 aaaataacct tcaggccccg tggcttggga tcaccgcctt cttatcggtg gcgatattgt
    25441 tgtctttgct gatctttatt ggtgaagccg tccgcgacgc atttgatcct aataaggcgg
    25501 tgtagcatga cgcaaactct gttagcgatt gaaaatttgt cggtgggttt tcgccatcag
    25561 caaaccgtac gtacagtagt caatgatgtt tcactacaga ttgcggctgg cgaaacgctg
    25621 gcgctggtag gtgagtctgg ttcaggtaag agcgttaccg cgctgtcaat tttacgcctg
    25681 ctcccttgcc cgccggttga atatctctcc ggcgatattc gttttcatgg cgaatcgctg
    25741 cttcatgcca gcgatcaaac gttgcgcggt gtacgcggta ataagatcgc catgattttt
    25801 caggagccga tggtgtcgtt aaatccatta catcccctgg aaaaacagct ttatgaagta
    25861 ctttcactcc accgcgggat gcgtcgggaa gcggctcgtg gcgaaattct taactgcctt
    25921 gatcgcgtcg gtatccgcca ggcggcaaaa cggctgacag attatccgca tcagctctcc
    25981 ggcggcgaac gccagcgggt gatgattgcg atggcgctgt taacgcgacc ggaattatta
    26041 attgccgatg aaccaaccac cgcgctggac gtctctgtcc aggcgcagat tttacagctg
    26101 ttgcgcgaac tgcaaggcga gctgaatatg ggtatgctgt ttattactca taacctcagc
    26161 attgttagaa aactggccca ccgcgtggcg gtaatgcaaa acggtcgctg tgtcgagaaa
    26221 aataacgccg ctacgctgtt tgcctcaccc actcatcctt acacacaaaa gctactcaac
    26281 agtgaaccat ctggcgaccc ggtgccattg ccagaacctg cctctacgtt gctagatgtt
    26341 gaacaacttc aagttgcctt ccccattcgt aaagggatct tgaagcgcat tgtggatcat
    26401 aatgtggtgg tgaaaaacat cagttttacg ctacgggcgg gtgaaacact gggtttagtg
    26461 ggcgagtccg gttccgggaa aagcacgacg ggactggcgc tgctgcgact gattaattct
    26521 catggcagca tcgtctttga cggtcagcca ctgcaaaatt taaatcgccg ccagctgtta
    26581 cctattcgtc atcgcattca ggtggtattt caggatccaa actcctcact caacccacga
    26641 ctcaacgttt tgcaaattat tgaggaaggc ttacgggttc accagccgac gctttctgcc
    26701 gcacaacgcg aacaacaagt gatagccgtg atgcatgaag tgggattaga tcctgaaaca
    26761 cgccaccgtt atccggcgga gttctctggt ggtcagcggc aacgtattgc gattgccagg
    26821 gcgttaattc ttaagccctc gctgatcatt cttgatgaac caacatcatc actcgacaaa
    26881 acggtgcagg cgcaaatatt gacgctattg aaatcattgc aacaaaagca tcaactggcc
    26941 tatttgttta tcagccacga tttgcacgtt gtccgcgcgt tatgtcatca ggttatcgta
    27001 ctgcgacaag gggaagtagt ggaacaagga ccgtacgcgc gcgtgtttgc cgcaccgcag
    27061 caggagtata cgcgtcagct actggcgttg agctgacgct taaaaaggat tgtagtttga
    27121 aaagggttcg gcaattgcca caccaaaatt tttcaatcgg catgttgcag caaattcgtc
    27181 ctggcgattt acaaacaggc acggctcacc ttcacactcc agcaccgaac atggaacttc
    27241 gatgtcgctt aacgcctggc ttagtttgta catcaccgac catgcattat caccatccgg
    27301 gctaagcaat ttaagcgcca ccaggctgtt gtcaccgcac ggtccatttt gcggaatcgg
    27361 ttcaaccttc gaacctgcga aaccagcaga ccagcgatag ttctgcggca gtcgatggac
    27421 gattgagagt tgtaatgaag tcacttattt tccccggaag tacattttac ttcacaattt
    27481 gtttacatca attttaacac atcatcaaca atccgtcttt atacagatcg taaagatgat
    27541 gcctcgcctg cgtggcctct atggctctga tttaaataat atctgcgggc gtgctcacgt
    27601 ttgcggggct atatgtaccc gtttctgcga tctaactcaa cctttttaac tacaatgatg
    27661 tgacttttta cataaattga ttttacataa aataaacata tatcggggga atgatagatt
    27721 tgtggttgac atgcatcaac aaaggaagcc ttttagcttc ctcgttgtgc aatagatcac
    27781 cgttttttcg gccgactggc gtacaaacag aagagaatgg agctggttgc gcagaatgca
    27841 attgaccaaa tcatcggcca tgcagagtta aaggtcgcaa gagaaagtaa tgcgccaaca
    27901 attgccccta tgccgaaacg gaaggttcct gccagcgaag atgccgttcc cgccatatgg
    27961 ggaaactcat caagaatgac cgccatcgca ttggatgaca ccatcgacac gcagcccaca
    28021 aacgccgcaa cgccaaccac cagcgaccag aatcccagcc ccagcagtgc actgatgacc
    28081 atccacgctg ccataataaa ctgtatccac aaccccgagc ggaacatatt taacgcgcca
    28141 atacggcgga cgaagcggct gttaaagatg gtcatcacga acagaaaaag aatgtttagc
    28201 gcaaagtaat aaccaaagtt ttccggcgcg acgtggttga tttcaatgta gacaaacggt
    28261 ccggcgctta agaatgagaa catcccggca aagctgaaac cgctggccag catataactc
    28321 aggacacgct tatgacggaa cagcgccgca aagttaccaa tagtggtacg aatgtgaaaa
    28381 ggctggcgac gctccggcgg taaggtttct ttgatcagaa agaagatcat tgccgaagcc
    28441 agaatcgccg ccacggcgag gatccagaag atgtaatgcc agctcagcca caccagcacc
    28501 cagccgccaa ctatcggtgc catcagcggt gcaatggtcg tcaccagcat gacaaacgac
    28561 atcatccgtg agaactcttc tttcgggtag atatcacgca tcaaagcgtt gataaccacg
    28621 ctggccgcag ccgcagccag cccgtggaag aaacgcatca caatcagctg atcgatggtt
    28681 tgcgccaacg cacacgccac cgcggcggcg gcaaacacca gcgtaccgcc aagaaccacc
    28741 ggcttacgcc cgaagctgtc tgccatcggc ccgtagatta actgccccaa cgcaaagccc
    28801 agaatataag tactcagggt catctgcgta ctgcccgccg gtacaccaaa ctgcgctgaa
    28861 attaccggta gcgcaggcag atacatatca atcgacagcg gcatcaacat ggccagcagg
    28921 ccaaggataa aaacaatagc aaacgacgaa tgctgtcggg tggtcacaac gggctcctga
    28981 aagtcattga aaagttagac gacgctggca atttcttctt cagttaacgg acgatattca
    29041 ccgggggcta aatcagcatc cagcgtaatg ccgccaatac gttcacgatg cagctcaacc
    29101 acatggttac ccacggcggc gaacatgcgt ttcacctgat gataacgccc ttcgctgatg
    29161 gtcagacgaa cctgcgttgg ggtaatcact tccagcaccg ccggcttagt gagatctttt
    29221 tcgttatgca gctgcacgcc tttagcaaat tgctctgccg tatcgtcagc tacgggtgat
    29281 tccagcgtta ccagataggt cttctcgcaa tgatggcgcg gggaagtaat gcggtgcgac
    29341 cactgaccat catctgtcat tagtaccaga ccggtggtat cgatatccag acgccctgca
    29401 gcgtgcagtt tccacgctac cggttcatca agaaaataga gcaccgttgg gtgatccggg
    29461 tcgtccgtgg agcaaacata gccctgaggc ttattgagca tgaagtaacg tggaccgtgt
    29521 tgctgcgcca gcgggttgcc atcgtaagcg acatcatgtt cagggagcag tttgaacgct
    29581 gcattacgga cgatttcgcc atcgacggtg acacgattgc cgcggatttc acgcccggca
    29641 atagcacggc taacgccgag ttgctgtgcg ataaatttat caagtcgcat gtgtgtgatt
    29701 ttgcctgtaa aaaacggagg tcgggtaatg acccgaaaat ctgaactgtt gtctgcccag
    29761 tatagcggtc taataacttc cctcaaggga aaaagatccg tggcatacta ttagcagaat
    29821 aatctaccta cgcgagacca tgatttttac actccgccca tatcagcaag aagccgtgga
    29881 tgccacgctc aaccattttc gtcgtcataa aacccctgcc gttatcgtgc tgcccaccgg
    29941 tgcaggtaaa agcctggtga tagcggaact ggcgcggctg gcgcgtggtc gcgtgctggt
    30001 gctggcacac gttaaagaac tggtggcaca aaaccatgca aaatatcagg cgctggggct
    30061 ggaagccgat atttttgccg ccgggctaaa gcgcaaagag agccacggta aagtggtatt
    30121 tggcagcgtg cagtcggtcg cccgtaatct tgatgccttt cagggtgaat tttcgctgtt
    30181 gattgtcgat gaatgccacc gtattggtga cgatgaagag agccagtatc agcaaatcct
    30241 cactcacctg acaaaagtga atccccactt acgcctgctg gggctgactg ccacgccttt
    30301 tcgactgggc aaaggctgga tttatcagtt ccattatcac ggcatggtac gcggcgatga
    30361 gaaagccctt ttccgtgact gcatttatga gctgccgctg cgttatatga ttaaacacgg
    30421 ctatctgacg ccgccagaac ggctggatat gccagtagtg caatacgatt tcagccgctt
    30481 gcaggcacag agtaacggac tgttcagcga agccgatctc aaccgcgagc tgaaaaaaca
    30541 gcaacgcatt acgccacaca tcatcagcca gattatggag tttgctgcaa cgcgcaaagg
    30601 ggtgatgatt ttcgccgcga cggtcgaaca cgcaaaagag attgtgggat tactgcctgc
    30661 cgaagatgca gcactgatta ctggcgacac acccggcgct gagcgcgatg tgttaattga
    30721 agattttaaa gcccagcgtt ttcgctatct ggtcaacgtc gcggtactga ctaccggatt
    30781 tgacgccccg cacgtcgatc ttatcgccat tctgcgccct accgaatcag tgagtcttta
    30841 ccaacaaatt gtcgggcgcg gtctgcgtct cgctcctggc aagactgatt gcttaattct
    30901 tgattatgcg ggtaatcctc acgatctcta cgcgccggaa gttggtacac cgaaaggcaa
    30961 aagtgacaac gtcccggtac aagttttctg ccctgcctgc ggttttgcca acaccttttg
    31021 ggggaaaacg accgccgacg ggacattgat tgaacacttt ggtcgtcgtt gtcagggatg
    31081 gtttgaagat gacgacggtc atcgcgaaca gtgtgacttc cgtttccgtt ttaaaaattg
    31141 cccgcaatgt aacgcagaaa atgatattgc cgctcgccgc tgccgcgaat gtgacacggt
    31201 actggttgat ccggatgata tgttaaaagc ggcgctacga ctgaaagatg cgctggtatt
    31261 acgctgtagc ggcatgtctt tgcagcatgg acacgacgag aaaggcgaat ggttgaaaat
    31321 cacctattac gatgaagacg gcgcggatgt gagtgagcgt ttccgtctgc aaacgcccgc
    31381 ccagcgtacc gccttcgagc agctttttat ccgcccgcat acgcgcacac cgggcatccc
    31441 tctgcgctgg atcaccgccg ccgatatcct cgcccagcaa gcattactgc gacacccgga
    31501 ttttgtcgtc gcccgcatga aaggtcagta ctggcaagtg cgtgaaaaag tgttcgatta
    31561 cgaaggtcgt tttcgtcggg cgcacgaatt acgcggttaa tggtagtttt gattgatgta
    31621 taaggcgatt gagtatagaa tctcgcccgc ttttgcatac gcaaagcaga tcacttacct
    31681 gttgctgggt cgcctgtagc aggattaatt taagagagaa agaaatgttt actatcaacg
    31741 cagaagtacg taaagagcag ggtaagggtg cgagccgccg cctgcgtgcc gctaacaagt
    31801 tcccggcaat catctacggt ggcaaagaag cgccgctggc tgtcgaactg gatcacgaca
    31861 aagtcatgaa catgcaagtt aaagctgaat tctacagcga agttctgacc atcgttgttg
    31921 acggtaaaga aatcaaagtt aaagctcagg acgtacagcg tcacccgtac aaaccgaagc
    31981 tgctgcacat cgacttcgtt cgcgcttaat tgctgaataa gttgtaaaaa accccgctcc
    32041 ggcggggttt tttgtctctg caaattatgc ctgattacgg tattgctatt ttttgcaggc
    32101 cagataaggc gttcacgccg catctggcat tcagaacaca aaaccgatta attgccgcca
    32161 gacgtccggc gctgcaattg atcgcgcaag ttcggcggcg tgcctttaat ggtcagggta
    32221 tcggttgctg gatcccagaa gatgcgttca cctaacagca tcgcatcaaa gttaatagtc
    32281 aagccaccgc cgctaccggc aaacttcgtc aactgacgca gtgtgctacg gtctgccggg
    32341 aagctctctt ccagttcgta gcctttttca gccgcaaact ccgtgaagct cacttcgctg
    32401 acgcctgcca gctctttcga cagcgattcc agttcaatct cttcacctgc ctgcagctgc
    32461 tcattacagt agctataaac ctgctggcgc acgttctgtc gctctgcttt atccagttgt
    32521 gcttcggcag tgaaatcatc gaccgcttgc aacagcccac ggttttgcgc tttcgcgttt
    32581 aacccttcgc tggcaccgag gaaatccata aagaaatccg ccactttgcg ccctactcgc
    32641 cctttcagga aagtgagata gcgggtcgac tctggattgg tttcccactc ggttaaatcg
    32701 atacgcgcaa caatatccgc atgattgata tcaagataat gggttgggtt gatgtcgaga
    32761 ttttcgttaa cacgcatact gctcaggttg ctcagaactg ccaccagcag atactccacc
    32821 gccagatagc gatagtggca aaacagcacg aatccaccgt cagcaaaagg atatttcgct
    32881 aattcgtcac gcaggcgacc ggttgccgcg cggctaaatg ccagaaagtc ctcttcgccc
    32941 tgacgctgta agcgcagcgt ctgcgccagt tcactctctt cgctaaacaa gccgtaagct
    33001 ttatttttgg cgctatagac ccgatgcagt tctgccacca tctcaacgac ggtttccgtc
    33061 ggttccagca atgaatcgcg caacaccagc tcaaggttct gctcatcacg cttgataagc
    33121 tggtgcagag caatctggtt gatatccaga ctcatgataa actctccttt aagaccgggc
    33181 ggtattcaac caccgccggt taagcgacgc aataaaagat ggcggtcatt ttacagaagg
    33241 cggcgtaacg agaatgattt aacgcataaa aaagcagaaa aaaaaccgtt gctacggtaa
    33301 tatgttgccc tttcatcaac taactgattt cgatttatgc cacaaatttc ccgctacagt
    33361 gatgaacagg ttgaacaatt gctcgcagag ctgctcaaca tactggaaaa acataaggct
    33421 ccgaccgacc tttccctgat ggtgttaggc aacatggtca ctaaccttat caacaccagc
    33481 attgccccgg cccaacgcca ggcaattgcc aactcttttg cccgcgcctt acagtcctct
    33541 atcaacgaag acaaagcgca ctaagggaaa cagataacag gttatggtaa ctcatcgtca
    33601 gcgctaccgt gaaaaagtct cccagatggt cagttggggg cactggtttg cactgttcaa
    33661 tattctgctt tcgctcgtca ttggcagccg ttacctgttt atcgccgact ggccgacaac
    33721 gcttgctggt cgcatttatt cctacgtaag cattatcggt catttcagct tcctggtgtt
    33781 cgccacctac ttgctgatcc tcttcccgct aacctttatc gtcggctccc agaggctgat
    33841 gaggtttttg tccgtcattc tggcaacggc gggaatgacg ctattactga tcgacagcga
    33901 agtctttact cgtttccatc tccatcttaa tcccatcgtc tggcagctgg ttatcaaccc
    33961 agacgaaaat gagatggcgc gcgactggca gctaatgttc atcagtgtgc cggttatttt
    34021 attgcttgaa ctggtgtttg cgacgtggag ctggcaaaag ctgcgcagcc tgacgcgtcg
    34081 tcggcgcttc gcgcgcccgc tggccgcatt cttatttatc gcctttatcg cctcgcatgt
    34141 ggtttatatc tgggccgatg ccaacttcta tcgcccgatc accatgcagc gcgctaacct
    34201 gccgctttcg tacccgatga cggcacgacg ttttcttgag aagcatggcc tgcttgatgc
    34261 gcaggagtat caacgccgtc ttattgagca aggcaatcca gacgccgttt ccgttcagta
    34321 cccgttaagc gaactgcgct atcgcgatat gggcaccggg cagaatgtgc tgttgattac
    34381 tgtcgatggc ctgaactact cacgcttcga gaagcagatg cctgcgctgg caggttttgc
    34441 tgagcaaaat atttcgttca cgcgtcatat gagctccggc aacactacag acaacggcat
    34501 ctttggcctg ttttatggca tctcgccaag ctatatggac ggcattttgt cgacccgtac
    34561 gcctgcggcg ttaattactg cgcttaatca gcaaggctat cagctggggt tattctcgtc
    34621 agatggcttt accagcccgc tgtatcggca ggcattgttg tcagatttct cgatgccgag
    34681 tgtacgcacc caatccgacg aacagaccgc cacgcagtgg atcaactggc tgggccgcta
    34741 cgcacaagaa gataaccgct ggttctcgtg ggtctctttc aatggcacaa acattgacga
    34801 cagcaatcag caggcatttg cacggaaata tagccgggca gcaggcaatg tcgatgacca
    34861 gatcaaccgc gtgctcaatg cactgcgtga ttctggcaaa ctggacaata cggtggtgat
    34921 tatcactgcc gggcggggta ttccgctgag cgaagaggaa gaaacctttg actggtccca
    34981 cggtcatctg caggtgccat tagtgattca ctggccaggc acgccggcgc agcgtattaa
    35041 tgcgctgact gatcataccg atctgatgac gacgctgatg caacgcctgc tacatgtcag
    35101 cacacctgcc agcgaatatt cgcaaggtca ggatttgttc aaccctcaac gccgccatta
    35161 ctgggttacc gccgcggata acgatacgct ggcaattacc accccgaaaa agacgctggt
    35221 gctgaacaat aacggtaaat accgcactta caacttacgt ggtgaaagag tgaaagatga
    35281 aaaaccacag ttaagtttgt tattgcaagt actgacagac gagaagcgtt ttatcgctaa
    35341 ctgattaatt ataaatcagt tagcaaaata tcttacttgc aatcggtgtg gaaaacggta
    35401 gtattagcag ccacgagtcg gcacgtagcg cagcctggta gcgcaccgtc atggggtgtc
    35461 gggggtcgga ggttcaaatc ctctcgtgcc gaccaaaaat cccaagaaaa ccaaccaatt
    35521 gcggttggtt tttttatatc tgcatttaat tcgataaaca gacagcgaca catcacggcc
    35581 tgtttatttt ctgttatcag aacgtccaga ctacacccgc ctgagttgtg ctgctttctt
    35641 cacgggagaa acggctgtcg ataccaagcg ttagccccgt caatgtttgc tcaaagccag
    35701 cgcctacatc agtggtcacg ttgttgcggc tgttaattgc cgaactccac atcgccgtat
    35761 cgtaattaac gccttttacg ctaccaagac gcccacgcac ggtgtccagt tctgcatcaa
    35821 ataccagcgg ttgtgctgcc gccatattca gcacatccgt ggttgaaggg ggcatttgtg
    35881 cgcgattctc tgcgagactc cagctatggt tgccattatc cagcaaggta tattcatacg
    35941 tactgatatc aacaacgcct ccggcattgc ccaacgtaaa tgccgcatca ccgcgcccgt
    36001 tgttaccagt gtaaggttat ctcctgctgc cgggctggca ccggtgtccg tcacgaatat
    36061 tttgaaatca cctgttgctt taacctccag tagccctccc ttttctataa cggcaaagac
    36121 ggcaatccca tctttttcaa caattaagcg ccctccttta atcgtgggat cagtcgccgc
    36181 gccataaacc tgtagcactc cattattgat cgtagtctgg tttaccgcgc cacccatttg
    36241 aacaatttga taaccatcat ttaatactga gtattctgcg gtgccattta tttcaatgta
    36301 ctgatatccc ccgttaattt cagtattact acttacacca aattcttata tatgctgttc
    36361 tccgccagga ttgattttcg tattattggc ggtccaataa accagctgga tatctttttc
    36421 caggacaaca ccatcaactg gctcaccata ctcaacagtt gatgccaata ctatgggcga
    36481 atataacgct gtagccacca gaacagatat tgcggaacga caaagagaaa cagaaccaga
    36541 ttggtgcatt aagctttcat cctatgaaat taattgctgt taaaagcatt ggggacagaa
    36601 aatacccata gctccatacc cggagtcagt tttaaaaaac tgtttaaaga aatgcacaag
    36661 tattgtgatt gattttttag ttgtttttcc tgatgagaag ctgatgcaaa attccgtctt
    36721 tataatgaag atgatgccaa agcgaacgac aaagttgtag ttttcactac atgtccatac
    36781 ataaaatggg gtaacattca cgcgcctggt agcgttacca acgctacgct caaacataat
    36841 gattctaata aaacctcagg agactactat gcctgaagca acgccttttc aggtgatgat
    36901 tgtggatgat catccactta tgcgacgcgg tgttcgtcag ttactggagc ttgattctgg
    36961 ctttgaagtg gtcgccgaag cgggcgacgg cgcaagcgcg atcgatctgg cgaatagact
    37021 ggatatcgac gtgatcttgc tggatctcaa tatgaaaggc atgagtggcc tggataccct
    37081 caatgccttg cgcagggatg gcgttaccgc gcaaattatt atcctgaccg tatcagatgc
    37141 ctccagcgat gtctttgcac tgatagacgc aggcgcagac ggttatctgt tgaaagacag
    37201 cgacccggaa gtgttactgg aagcgattcg tgccggagcg aaaggcagca aagtctttag
    37261 cgaacgcgtc aatcagtact tacgtgaacg tgaaatgttt ggcgcggaag aagacccctt
    37321 cagcgtgctg acagaacgcg agctggatgt tctgcacgag ctggcacagg ggctgtcaaa
    37381 taaacagatt gcctcggtgt tgaatatttc cgagcagaca gtaaaagtcc atattcgcaa
    37441 tctgctgcgt aaactcaacg tccgctcacg cgtggcggcc accattctgt tcctgcaaca
    37501 acgcggggca caataaaaat agcccgatgg catcaccatc gggccacttt ttatttactc
    37561 tcctgcggcg acaaatgttg catcgcctgc gcgatactac gttcgatcac cgcacggcga
    37621 gtatcgttgg caggtaagag tttcaacatc atctcccacg cggcaacggc ttcgccaaat
    37681 cgttgctgct caaaggcatt aaatgcatac atgcttaaca cacggatatt gctatggtcc
    37741 gttctcacca actggcgcag cagttcgccg ccgaggcggt tgtcgttggg atcagatgaa
    37801 cgtgtcaacg cttcagcgta tcccagtgca gcatcgctgt ttttcggatc gaggcgatac
    37861 gcagtggcgt atgcatcggt ggcgatactg gcgttaccca gcgccatgcc aacgcggccc
    37921 aacataatcc agccttctat atctcccgga tttttttgca gttgagtacg catccccagc
    37981 gcaaggcgcg acatctcttc ttcgttgagc ggatcggctt tcggatccag cgccctgtcc
    38041 agtaacgccg gagcctgtgc cgtggcctgc tgccagattt tcacctgctg ataattgcca
    38101 gtctggtagt agctgacgcc agccacaatt aacgccacca caatacccgg cagataaaca
    38161 acatatccgg cacgcttacc ttccggcacg ctttgttctg gaaatgcttc cggcaccacg
    38221 cgtacccgac gccgcgaacg ggcgtatatg acccaaccgc caatgccaat agccactact
    38281 ggcagcaccc acagcagcac ggtcagcggc gttaacggcg gatcgtaagt gacgaagttg
    38341 ccgtaacgcg ccaccatata atcgacaatc tctttcttac ttttgccttc ctgcatcagt
    38401 tcatacactt tctggcgcag gtcggtggca atcatcgagt tggaatcggc aatgctgttg
    38461 ttctggcatt tcgggcagcg cagttcttca gtgagctgac ggaactgttg ttcctgtgct
    38521 tcatctttaa actgcaacac gtcgatggtc gccagcgctg agccggagat catcagcatc
    38581 agcacgccca ataaaaacct cattgtgcgg cctccttact gtatttctcc cacagtggct
    38641 tgatctcttc ttcccagacg cgaggattga gatcgcccgc atggcgatag cgaatgatgc
    38701 cgttgccgtc aataaggaac gtttctggcg cgccatagac accgagatcc agccctaaca
    38761 tgccgtcgcc atcaaacagg cttagcgcgt aaggattgcc cagctctttc agccagctga
    38821 ttgccttctg gcgatcgtct ttatagttca tgccgaccac gcggatgccc tgcgcagaaa
    38881 gctgattcag atattgatgt tccgcacggc aggtcggaca ccaggtcgcc cagacgttaa
    38941 gcagcaccgg tttgccctga gtcagcacat ccgcctgata aaactgcccc ggattgtcca
    39001 gtgattcgag gcgaaacttc ggcactggct tgccaatgag cgccgattcc agattggtcg
    39061 gatcatcccc ttcggcatta cgcgccagct gccacagcag cgccgcggca atcgccagga
    39121 agataatcaa cggaattaac aataccttgc gcttcatgca gcctccggca cagttttctg
    39181 cgggttcacg cgcttacgat agcgaggatc aagcagacac agcagtccgc ccaacgccat
    39241 catcagcccg cccgcccaaa tccagcgaac aaatggtttg tagtaaagac gcacggccca
    39301 cgcgccgttt tccagctctt caccgagcgc cgcgtacagg tcacgcgtga tgccaccgtc
    39361 aatcgccgct tcggtcatca tcgacccggc agtgttgtaa taacgttttt ctgcatacag
    39421 cactgtttcc ggcttgccat ctcgcgttac gccgatagtc gccacaccgc cacgccagtt
    39481 cggaccagtc acctctttga catcacggaa ggtgaagcga tattcatgaa tatcgacgct
    39541 atcgccggac ttcatgcgca catcacgctc aacgctatag ttctgactaa aagcaatgcc
    39601 aacaattgtc actgccagcc ccaggtgagc cgccaccatc ccccaataac taaaggtggt
    39661 tttcgtgccg cgtgaaatac gcagcgcagc ttccgcaatg gccagcactg caatccagca
    39721 ggccattgcc aggccgagca ccgtcatcgc cacaactttg ctttcgaaca gccacggcaa
    39781 cagcagagac agcaccagcg tagagatgaa ggcgataatc aataaattgc ggattttacg
    39841 tgggcgatcg cgcccccagc gcaccagagg accgacaccc agcagtagcg caaacggcac
    39901 catcagccag gtaaacatgg tgttgaagaa cggttcgcca atcgaaatgc tgcccagtcc
    39961 cagttgcttg tgcaccagcg gcagcagcgt ccccagcaac accaccagca tcgcggcgac
    40021 cagcaaaacg ttgttcgcta acagcaaaga ttcccgcgac cacagcgcat tgtttacgcg
    40081 tgagcgaact ttgtgtccac gcgcggcaaa cagcagcagc gaaccgccaa tcaccagcac
    40141 cataaaagcg aggataaaca taccgcgcgc cggatcagac gcaaacgcgt gtaccgatac
    40201 cagcacgccg gaacgcacaa ggaaagtccc cagcagacac agcgagaagg cactgattgc
    40261 cagcagtaat gtccacgctt tgaagctggc acgctgttca gtgaccgcca gcgagtgcat
    40321 cagcgcagta cccaccaacc acggcataaa tgaggcgttt tctaccggat cccagaacca
    40381 ccagccgccc cagccgagtt cgtaataggc ccatgcggaa ccgagcacaa tgccgagcgt
    40441 caggaagatc cacgccgcca gcgtccacgg acgggtaaag cgtgcgtaag tgctgtccag
    40501 acgcccactc agtaaagaag caatggcaaa agcaaacgcc accgagaaac cgacgtaccc
    40561 catatagagc agaggcggat ggaagatcag ccctggatcc tgcaacagcg ggttaagatc
    40621 gcgaccttca atcggaaaat tcggcaacgt gcgtaagaac gggttagagg taaagagaat
    40681 gaacagcaaa aagccgacac tgaccatccc cattatcgcc agtacacggg ccacaatatc
    40741 cagcggaata cgctgactaa aaatcgccac cgcaaaggtc cagccgctca tcagcagcac
    40801 ccacagcagg agcgagcctt catgcgcacc ccaggttgcc gccacgcgat accacacagg
    40861 aagctgggta ttggagttgc tggcaacata ggtgacagta aagtcattca ccacgaaagc
    40921 attgaccagc accagaaatg cgccagccac agacataaat agcagccagg caaacaagcg
    40981 cgaagaagcc atcatgcgcg catccccgcg cgccacgccc catagcggat acacggacag
    41041 caacagcgca attcctagcg ccaggcacag cagtccgtta ccaatttctg gcatcatgat
    41101 gctgggccct tataaacact cgccgggcga cggtgattag cttccatcgc tttctcgact
    41161 tctggcggcg tgtagttttc atcgtgtttc gccagcactt ctttcgcgag gatatgattg
    41221 cctttttcca gttcgccctg caccacaacg ccctgccctt cacggaacag atccggcaaa
    41281 atgccttcgt aagagacatc tactgaacct tcagcgtcgt aaatggtgaa agttactttt
    41341 agcgaattgg gatcgcgctg cacactcccc ggcatcacca tcccgccaac gcgcagacgc
    41401 tgaccgactt ccggcatctg ctgagtttca cgcttgccgt agagaatctc ccccggcgta
    41461 taaaagagat caatattcga gcgcagcgca tatagcacca gaccgatagt cagcgccagc
    41521 cccgccagca cggcacaggc aatccacaag cggtttttac ggcgaatatt catgcagcct
    41581 cctgctgttg cgcagcacgt aaacgcgcct cacgcgcccg ctgttgcgcc acgccacgca
    41641 gaattgcgcg atgttgcatc accgagtgca cgaccaaaac caccagcgga ataacggtca
    41701 tcaccaccgc cagccagaca aaaaaggcgt aaccgcccat tgcgaaaaat tcattccagg
    41761 aagcaaatgc aggggtcatt tacggcctct tttcagtatc agttcactca cccacggacg
    41821 gcgtttttcc atcagcaaaa tcaaattacg catccgcatc agcgtcagcg tggcagacag
    41881 gagcaggaag ccaaaaatcg accagcgcag cggcgaacgc atcgccggat cgatactttg
    41941 ctgcattcgc gttgatccct gatgcagggt gttccaccac tccacggagt aatgaataat
    42001 cggcagattc accacgccaa tcagcaccag gatacctgcc gcacggccag ccagacgacg
    42061 gtcgtcgaag gcgtgccaca gggcaatgac accaacatac agaaacagca gcaccagttc
    42121 agaagtcaga cgtgcatccc atacccacca ggtgccccac atcggttttc cccatgcaga
    42181 gccggtaacc agggcaataa aggtaaacac ggcaccaatg ggggccatcg ccgccaccgc
    42241 caggttggcc attttcatct gccagaccag gccaataaac gctgccaccg ccattgatgc
    42301 ataaatgccc atcgaccaga tcgccgcagg cacatgcagg tagataatgc ggtagctatt
    42361 tccctgctga taatcagccg gagcaaagcc gaatccccag atccagccga cggtaagcac
    42421 gaccacactg gcaattgcca gccacggtat aaaccagcca cagatttgat acagccgtgg
    42481 tgggatcgcc agttgatgca gtgttttcca catagtttcg ataccagact cgaacaaaaa
    42541 tcagtaatcc agcgttattg aatgctgatt cgtaacgctg ccgccgtcgc aaaaggactt
    42601 aatgtcgcgg tgcctgccag caacgcgcct aaaattgcca gatacccatc aacgggcaaa
    42661 tgcatagaag ccgcgtccat cgcggcggtg gcaaagatga gtaatgggat agtcagcggt
    42721 aacaccagta tgctgagcag cacaccaccg cgcttaagtc ccactgtcag cgccacgccc
    42781 ggcgcaccaa gaaagccaag cgtaggggtt cccagcagta gcgttaacgc catcacttgc
    42841 cagccataaa catccattcc cagtagcatt gctaccaggg gcgaaaggat gagtaacggc
    42901 agaccggtta ccatccagtg cgccatcacc ttcgccagca cagcggcggg caagggtaac
    42961 ggcaacaaca tcaattgttc aagactgccg tcctgcaaat cgtcacggaa cagtcgctcg
    43021 agtgccagca aggatgaaag cagcgcggca acccagataa tgcccggcgc aatacgcgcc
    43081 agcagttgcg gctccggacc gatactgagc ggaaaaaggg taattacaat caggaagaac
    43141 cacagcgggt tggcgatttc ggcgctatgg cgaaacgcta cgcgcagctc aagacggaaa
    43201 atgcgccaga acatcatgcg gcccccgttt gcgtcagtga aatgcggcga attttacttt
    43261 cagcaacgtt gagcggctgg tgggtagtca gaatcacaat ccccccctgc tccgtatgct
    43321 gcgccatacg ctgggtcaga cgatcgacgc cgttaacgtc aatcgcggta aaaggctcgt
    43381 cgagtatcca taacgtggca cgggtcagcc acagacgcgc taaagcgacg cggcgttgtt
    43441 gcccggcaga aagttgattt acaggaatat cttcgaatcc ggcaagcccg gcctgcgcca
    43501 gggcttccag acattgtgcg gtatcgccat cgcgatgata aaagtgcaga ttttctaacg
    43561 ccgtcagccg ggttttgatc cccggctgat ggcctatcca taacaggttt tgatggtagc
    43621 tgtcgcgtac ctgatgcaag ggctgccctt gccagagaac ctcgcctgcg tcagggcgag
    43681 acaaccccgt cagcaaacgg agaagcgttg tcttccccgc gccgttgcta ccggtgattt
    43741 gtacccactc tcctgcgttc agcgtaaatg ataaaccact aaataaggtt cgttcatccc
    43801 gctcgcaaag taactctctg gcttcaagca tacccacgca ataaccctgt taaaaacctg
    43861 gctcgacttc acgcatgtcg ggcagcttgt gcgctatccc tttatggcaa tcaatacagg
    43921 tttgcccatc tttcaccgcc tggtcatgca tcttcgcggc aaccgatttc tgggcggttg
    43981 tatccatata ctcgaagttg tgacagttac ggcactcctg cgagttgttg tccttcatgc
    44041 gccgccactc attctgtgcc atcgtcaggc gatgagcttc aaatttctgc ggcgtgtcaa
    44101 taacgccaaa aattttacca tacagctctt tacttgcttt gagcttgcgt atcatctttg
    44161 gcacaaactc gtgcggaacg tgacaatccg gacaggtcgc acggacgccg ctacggttgt
    44221 tgtagtgcac ggaatccatg tattcctgat acaccgtgtt acgcatttcg tggcagctaa
    44281 tgcagaactc ttcggtattg gctttttcca tcccggtgtt aaagccgccc cagaagacga
    44341 tgccgccgac aaaaccgatc aacagcagcg tccctagcgc cagacggctg ggggtacgcc
    44401 accatttcca caggcgctta atcagaccag gcttacggtc agaatttccc ataataacct
    44461 cttatttccc gtaacctttt gatggggtaa aggtattccc cacgattggc gcggtatcgg
    44521 cctgcggtac gtgacattgc agacagaaat aacgacgcgg agccacttcc gcgcccactt
    44581 tgccgtcgct gtccataaag tgagtaggac taatgcgcgg cgcgccagtg gtgcgataac
    44641 tttcgacacc gtggcactgc aagcagcggt tggtattggt cgttacctga taaccttcaa
    44701 cgctatgcgg gatcattggc ggctgattca catagttcag cggcatccga tcctgctctt
    44761 ttggcatccg aatggcccct tcctgcgtcc cggatacttc cggcgactgg ctaaaatcga
    44821 cgccattagc cgcccaaacc gcgccgctta ccaccagggc cagcatcgcc gtccattgac
    44881 acagcgcttt cttcaggtca tggcttttca tgatttcgct cccgaactcc atcgtgtagt
    44941 tattgtaaat acatcctcag aacagacatc cacgcagcga ccgcacgtca tgcaatcgcg
    45001 gctggtgacc tgcaccgggc tttgttcatc cagcaccggg gcacgtagca catgcggttc
    45061 cgggcaaaca tgaaaacaat ccatacagcg gttacatttc tgccgatcgg tggccgcaac
    45121 ggtaatcaca cctttgctac ccagcacgcc atacagtgca ccaaccggac aaatgtgccc
    45181 gcaccagccg tgttcaacga ccagtagatc aaataaaaac agcgcgagaa taagcagcgc
    45241 gccgctgccg aagcccatta ccaggctacg ccccatcaga gaaacggggt tgatccattc
    45301 ccagatgagc gtgccagtta aggccgatcc caccagaatg accaccaaca gcacgtagcg
    45361 aatatggcga gggatcgtcg cagactgatt gaggtcaaat cgtctgcgta accaatttgc
    45421 taagtcggta atcgggttca gcggacagac ccagctgcaa aataatcgct ttcctgccag
    45481 ggcgtagagc acggtgataa tcaccgcacc ggtcaacgcc accgtggcag gcagatgacc
    45541 gctggcaaga ctttgcagtg tcatcagcgg atcggtcagt ggaacggtgt cgaataacag
    45601 gctactgctg tagttgccgt gcaagatcca cacaccaaac cacggaccgc tcaaaaacat
    45661 ccccagcacg aagaactgac aaaggcgacg taacactaac caacggtgac tgcgccacca
    45721 gccttttttc tccagcgcct cgcgcccggc gtcacgttta cgatttgcca ttgttcccct
    45781 ccagccagcc gaagcggtaa tggtgaccta actccccttt cgccagtgac agcggtaaca
    45841 ccttgattgc cggttgttcc agcacgcaca ctttttcgca cttaccgcag ccggtacagg
    45901 cgtcgctgtg aaccgtcggc agaaagcggg cgtgcttacc ggttcgcgtg ttgcgctcca
    45961 gctccagggt gatggcctca tcaattttcg ggcattcgcg ataacaaaca tcgcagcgca
    46021 gcccctgaaa gttgagacag ttttcctggt ccaccagtac cgccagcccc atccgcgcgt
    46081 cgtcgatcga ttcgatctca cgatccagcg caccgctcgg gcacactttg gcgcacggaa
    46141 tgtcctcgca catttcgcaa ggaatatccc gtgcgacaaa atatggcgtg cccgccgaca
    46201 gaccagaggc cagcgtcgcc agttttaagg tgtcgtaagg gcaagcctga acacactgac
    46261 cgcaacgcac acaggcactg gcaaaggcgt tctcgtttat ggctccgggc gggcgcaacc
    46321 gcacaccaga tgcgcgtgcg gtttgctgtt gtaaccccag cgccacaccc acggcagcca
    46381 gcccgcctgc tgtgcgaacg acatcgcgca gaaagcggcg gcgaccattt tgaggtttcg
    46441 ctgaccggga cataatggct tacaccttct ccagtttgac cgcgcacttc ttgaagtccg
    46501 tctctttcga gagcggatcg gtcgcatcca gcgtcagttt gttaaccagc tgtgcggcgt
    46561 cgaagaacgg catgtacacc aggccctgcg gcggacggtt acgaccgcgc gtttcgacaa
    46621 tcgagatcac ttcgccacgg cgagaaacca ctttcacttt gtcaccacgg cgcagatcgc
    46681 gcgctttcgc atccagcggg tgaataaaca gaaccgcttc cgggaaggcg cggtgcagtt
    46741 ccggtacacg gcgagtcata ctgccggtat gccagtgctc caggacgcgt ccggtagaga
    46801 gccacaggtc gtactcttca tccggtgctt ccgccgcagg ttcgaacggc agcgcgaaga
    46861 tcaccgcttt gccatccggt ttaccgtaga acttgtagcc ttcgcccgct ttcacgtacg
    46921 ggtcgttacc ttcgctgtaa cgccactgcg tttctttacc gttcaccacc ggccagcgca
    46981 gaccgcgtgc tttgtggtaa tcatcgaacg gcgcgagatc gtgaccgtga ccgcgaccaa
    47041 accatgcgta ctcttcgaac agcccttttt gcagatagaa gcccagctcg cgggattcat
    47101 cgttcagctg atcttccgcc agttcggata ccgggaattt gctcacttcc ggagtggcat
    47161 acagaacgtc gtacagcgtt ttgccgcgca gttctggttt cttcgccagc aactcttccg
    47221 gccacacttc ttcagttttg aagcgacggg agaactgaac taactgccag agatccgatt
    47281 ttgcttcacc cggtgcctgt acctgctgac gccagaactg agtacggcgt tcggcattac
    47341 cgtaagcgcc ctctttctct acccacattg cggtcggcag gatcaagtcg gctgccagcg
    47401 cactgaccgt cggatacgga tcggaaacga tgataaagtt gcgcggatcg cgccagcccg
    47461 gcatacgctc ttcattaatg ttcggtccgg cctgcatgtt attggtacac atggtccagt
    47521 aaacattcag tttgccgtct ttcagcgcac ggtcttgtgc caccgcgtgc agaccgattt
    47581 tcgccggaat ggtgccactc gggatattcc acttcttctc gcaaatatca cgatgtttct
    47641 cgttagtcac caccatgtcc gcaggcagac ggtgagcaaa ggtgcccact tcacgcgcag
    47701 taccacacgc ggaaggctgc ccggtcaggg agaacggacc gcaacccggc tgggaaattt
    47761 tgccggtcag caggtgcagg ttgtagacca ggttgttggc ccacacacca cgggtatgct
    47821 ggttgaagcc catcgtccag taggagatga ctttcttgtt cggatcggca tacagctgcg
    47881 ccagttgttc taactggtct ttcggcacgc cggtcatttc ggcggttttt tccagcgtat
    47941 attcagcaac gaaggctttg taatcttcaa agctcatcgg ttcggaggcg tcagaacccg
    48001 gattcttcgc tgctttttcc agcggatggg tcggacgtaa accgtagccg atgtccgtcg
    48061 ctcctttgcg caggttaacg tgtttgctga agaagtcctg atttatcgca ttgttttgaa
    48121 tgatatagtt ggcgatatag ttcaggatca ccaggtcaga ttgcggcgta aagatgatgc
    48181 cgttatccgc cagctcgaag ctacgatgct ggtaggtaga aagcaccgcc acggtgacgt
    48241 tctggttaga aagacgacgg ttagtgatgc gcgaccagag gatcgggtgc atctccgcca
    48301 tgtttgcgcc ccacagcaca aacgcgtcag cctgctcgat gtcgtcatag cagcccatcg
    48361 gctcatccat accaaaggta cgcataaagc caactactgc cgacgccata cagtgacgcg
    48421 cgttcgggtc gatgttgttc gaacggaagc ccgctttaaa cagcttggac gcggcataac
    48481 cttcccagat agtccactga ccagaaccga acataccgat cgattccggc cctttttctt
    48541 tcagggcggt tttgaatttc tcttccatca catcgaaggc ttgatcccag gtgattgggg
    48601 taaattcgcc ttctttgtca tatttaccgt ttttcatacg cagaagcggc tgcgtcaaac
    48661 ggtctttacc gtacatgatt ttgggcagga aatagccctt aatgcagttc agaccacggt
    48721 taaccggtgc gtccgggtcg ccctgacagg ccaccacacg cccctgctgc gttccgacca
    48781 gaacgccgca accagtaccg cagaaacggc acggcgcttt atcccattta atggcttcct
    48841 gctgaccaac aacggcgcgg gcaacgcccg gcacgctgag accggcagcc gccgcagcgg
    48901 ccgcaacggc gttagctttc ataaagctac gacgactgag tttcatggtg tttcctcacc
    48961 ttgctcttcc tgctggtgat aaaccagcga caccgccagc acgccctcta cgttgcgtac
    49021 tgactcaatg gtttggatca gcgtttcgct gtcttctgct tccaccacca caatcaactg
    49081 accgctcggc gcgtcgctga cagcaacttc acagccggga aaggcgttca gttgggtgct
    49141 gatgtctgaa attcgttcgc ttttggcctg cacgaccagg ctgcaaactt gccagttagt
    49201 gtgcatggag atactccgca gttatggctg ataccggaca gctggctgca catgctccac
    49261 atccgttgca aagttggcta ttaagttgcg gctggtagat cccggagagc gtcgggcgaa
    49321 agataattgc cattggctca cagctatcct gacagcggcg gcattcaact gactgatacg
    49381 ccaggcacgc gtccccaatg gtgaactgca aatcccaggc cctggtgtgg cgcggagaaa
    49441 atagcgattc ggggcaagcc tgcgcgcagg cgtaacagaa gctgcactcg ttatttttga
    49501 aattaacgct cggatagcca cccgcgccgc gttgcagaat gttgttttca caagcattaa
    49561 tacaggcgtc gcagcgggtg caatgggtca gaaaatgaga ttcatcaccg gaccagggcg
    49621 gacggatacc gttactggct ttgcgccagc gaccagtgag tatgccccga cgggatgcat
    49681 caatcttcac attgaccttc catcattaac gcgctctgaa aattgagagc gaccaaataa
    49741 accgcataat taataagcca tttttatagc cgctaagata ttaaaggatg tgtcaaagat
    49801 gcataccccg atcggggtaa aacctgtagc aggatcaaaa gagtggcggg aagcgtggca
    49861 aaaacggg
//
LOCUS       DADSZD010000014        49200 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.014, whole genome shotgun sequence.
ACCESSION   DADSZD010000014 DADSZD010000000
VERSION     DADSZD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.014"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            347..1690
                     /gene="argG"
                     /locus_tag="J1Z28_000725"
     CDS             347..1690
                     /gene="argG"
                     /locus_tag="J1Z28_000725"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBA7715583.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(1698..3323)
                     /locus_tag="J1Z28_000726"
     CDS             complement(1698..3323)
                     /locus_tag="J1Z28_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA7715584.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLIAVPFVLLIAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFFAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDNQGNPTPEGQG"
     gene            complement(3783..3869)
                     /locus_tag="J1Z28_000727"
     tRNA            complement(3783..3869)
                     /locus_tag="J1Z28_000727"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3833..3835),aa:Leu,seq:gag)
     gene            complement(3884..4216)
                     /gene="secG"
                     /locus_tag="J1Z28_000728"
     CDS             complement(3884..4216)
                     /gene="secG"
                     /locus_tag="J1Z28_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBA7715585.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4444..5781)
                     /gene="glmM"
                     /locus_tag="J1Z28_000729"
     CDS             complement(4444..5781)
                     /gene="glmM"
                     /locus_tag="J1Z28_000729"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003905541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBA7715586.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELTIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5774..6622)
                     /gene="folP"
                     /locus_tag="J1Z28_000730"
     CDS             complement(5774..6622)
                     /gene="folP"
                     /locus_tag="J1Z28_000730"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBA7715587.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLLDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6712..8646)
                     /gene="ftsH"
                     /locus_tag="J1Z28_000731"
     CDS             complement(6712..8646)
                     /gene="ftsH"
                     /locus_tag="J1Z28_000731"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBA7715588.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8746..9375)
                     /gene="rlmE"
                     /locus_tag="J1Z28_000732"
     CDS             complement(8746..9375)
                     /gene="rlmE"
                     /locus_tag="J1Z28_000732"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBA7715589.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9501..9794
                     /gene="yhbY"
                     /locus_tag="J1Z28_000733"
     CDS             9501..9794
                     /gene="yhbY"
                     /locus_tag="J1Z28_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBA7715590.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9950..10426)
                     /gene="greA"
                     /locus_tag="J1Z28_000734"
     CDS             complement(9950..10426)
                     /gene="greA"
                     /locus_tag="J1Z28_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBA7715591.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10674..12107
                     /gene="dacB"
                     /locus_tag="J1Z28_000735"
     CDS             10674..12107
                     /gene="dacB"
                     /locus_tag="J1Z28_000735"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBA7715592.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDGYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVTTLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELSFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12148..13320)
                     /gene="cgtA"
                     /locus_tag="J1Z28_000736"
     CDS             complement(12148..13320)
                     /gene="cgtA"
                     /locus_tag="J1Z28_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBA7715593.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKVEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13336..14301)
                     /locus_tag="J1Z28_000737"
     CDS             complement(13336..14301)
                     /locus_tag="J1Z28_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7715594.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLLVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTTALFPLADPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14428..14685)
                     /gene="rpmA"
                     /locus_tag="J1Z28_000738"
     CDS             complement(14428..14685)
                     /gene="rpmA"
                     /locus_tag="J1Z28_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBA7715595.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14706..15017)
                     /gene="rplU"
                     /locus_tag="J1Z28_000739"
     CDS             complement(14706..15017)
                     /gene="rplU"
                     /locus_tag="J1Z28_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBA7715596.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15276..16247
                     /gene="ispB"
                     /locus_tag="J1Z28_000740"
     CDS             15276..16247
                     /gene="ispB"
                     /locus_tag="J1Z28_000740"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBA7715597.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPGQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16480..16758
                     /gene="sfsB"
                     /locus_tag="J1Z28_000741"
     CDS             16480..16758
                     /gene="sfsB"
                     /locus_tag="J1Z28_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBA7715598.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16806..18065)
                     /gene="murA"
                     /locus_tag="J1Z28_000742"
     CDS             complement(16806..18065)
                     /gene="murA"
                     /locus_tag="J1Z28_000742"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBA7715599.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18120..18374)
                     /gene="ibaG"
                     /locus_tag="J1Z28_000743"
     CDS             complement(18120..18374)
                     /gene="ibaG"
                     /locus_tag="J1Z28_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015585657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBA7715600.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18534..18827)
                     /gene="mlaB"
                     /locus_tag="J1Z28_000744"
     CDS             complement(18534..18827)
                     /gene="mlaB"
                     /locus_tag="J1Z28_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBA7715601.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18827..19462)
                     /gene="mlaC"
                     /locus_tag="J1Z28_000745"
     CDS             complement(18827..19462)
                     /gene="mlaC"
                     /locus_tag="J1Z28_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBA7715602.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19481..20032)
                     /gene="mlaD"
                     /locus_tag="J1Z28_000746"
     CDS             complement(19481..20032)
                     /gene="mlaD"
                     /locus_tag="J1Z28_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBA7715603.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(20037..20819)
                     /gene="mlaE"
                     /locus_tag="J1Z28_000747"
     CDS             complement(20037..20819)
                     /gene="mlaE"
                     /locus_tag="J1Z28_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBA7715604.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20827..21636)
                     /gene="mlaF"
                     /locus_tag="J1Z28_000748"
     CDS             complement(20827..21636)
                     /gene="mlaF"
                     /locus_tag="J1Z28_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBA7715605.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21846..22823
                     /locus_tag="J1Z28_000749"
     CDS             21846..22823
                     /locus_tag="J1Z28_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBA7715606.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22837..23823
                     /gene="kdsD"
                     /locus_tag="J1Z28_000750"
     CDS             22837..23823
                     /gene="kdsD"
                     /locus_tag="J1Z28_000750"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBA7715607.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23844..24410
                     /gene="kdsC"
                     /locus_tag="J1Z28_000751"
     CDS             23844..24410
                     /gene="kdsC"
                     /locus_tag="J1Z28_000751"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBA7715608.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24407..24982
                     /gene="lptC"
                     /locus_tag="J1Z28_000752"
     CDS             24407..24982
                     /gene="lptC"
                     /locus_tag="J1Z28_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBA7715609.1"
                     /translation="MSKARRWVIIVLSLGVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRNKNAELIEKVRTSYEIQNKQTQP"
     gene            24951..25508
                     /gene="lptA"
                     /locus_tag="J1Z28_000753"
     CDS             24951..25508
                     /gene="lptA"
                     /locus_tag="J1Z28_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBA7715610.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25515..26240
                     /gene="lptB"
                     /locus_tag="J1Z28_000754"
     CDS             25515..26240
                     /gene="lptB"
                     /locus_tag="J1Z28_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBA7715611.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26288..27721
                     /gene="rpoN"
                     /locus_tag="J1Z28_000755"
     CDS             26288..27721
                     /gene="rpoN"
                     /locus_tag="J1Z28_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBA7715612.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27744..28031
                     /gene="hpf"
                     /locus_tag="J1Z28_000756"
     CDS             27744..28031
                     /gene="hpf"
                     /locus_tag="J1Z28_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007369896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBA7715613.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28149..28640
                     /gene="ptsN"
                     /locus_tag="J1Z28_000757"
     CDS             28149..28640
                     /gene="ptsN"
                     /locus_tag="J1Z28_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBA7715614.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28686..29540
                     /gene="rapZ"
                     /locus_tag="J1Z28_000758"
     CDS             28686..29540
                     /gene="rapZ"
                     /locus_tag="J1Z28_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBA7715615.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29537..29809
                     /gene="npr"
                     /locus_tag="J1Z28_000759"
     CDS             29537..29809
                     /gene="npr"
                     /locus_tag="J1Z28_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBA7715616.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            30022..30654
                     /gene="yrbL"
                     /locus_tag="J1Z28_000760"
     CDS             30022..30654
                     /gene="yrbL"
                     /locus_tag="J1Z28_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBA7715617.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30651..31379)
                     /gene="mtgA"
                     /locus_tag="J1Z28_000761"
     CDS             complement(30651..31379)
                     /gene="mtgA"
                     /locus_tag="J1Z28_000761"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBA7715618.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31376..32029)
                     /gene="elbB"
                     /locus_tag="J1Z28_000762"
     CDS             complement(31376..32029)
                     /gene="elbB"
                     /locus_tag="J1Z28_000762"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBA7715619.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32259..34595)
                     /gene="arcB"
                     /locus_tag="J1Z28_000763"
     CDS             complement(32259..34595)
                     /gene="arcB"
                     /locus_tag="J1Z28_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBA7715620.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34691..35620)
                     /locus_tag="J1Z28_000764"
     CDS             complement(34691..35620)
                     /locus_tag="J1Z28_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBA7715621.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHNKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVGLDRYLNEHGVQGSALGRPWLPPTA"
     gene            36295..40755
                     /gene="gltB"
                     /locus_tag="J1Z28_000765"
     CDS             36295..40755
                     /gene="gltB"
                     /locus_tag="J1Z28_000765"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBA7715622.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVEQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGADIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKALYCTEK
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40768..42186
                     /locus_tag="J1Z28_000766"
     CDS             40768..42186
                     /locus_tag="J1Z28_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7715623.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTDKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42370..43497
                     /locus_tag="J1Z28_000767"
     CDS             42370..43497
                     /locus_tag="J1Z28_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 domain-containing protein"
                     /protein_id="HBA7715624.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLILCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(43557..44021)
                     /locus_tag="J1Z28_000768"
     CDS             complement(43557..44021)
                     /locus_tag="J1Z28_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA7715625.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(44018..44893)
                     /locus_tag="J1Z28_000769"
     CDS             complement(44018..44893)
                     /locus_tag="J1Z28_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBA7715626.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIATAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEIL"
     gene            complement(44890..45579)
                     /locus_tag="J1Z28_000770"
     CDS             complement(44890..45579)
                     /locus_tag="J1Z28_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBA7715627.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVLVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45627..47117)
                     /gene="nanT"
                     /locus_tag="J1Z28_000771"
     CDS             complement(45627..47117)
                     /gene="nanT"
                     /locus_tag="J1Z28_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBA7715628.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNNLVKTKS"
     gene            complement(47226..48119)
                     /gene="nanA"
                     /locus_tag="J1Z28_000772"
     CDS             complement(47226..48119)
                     /gene="nanA"
                     /locus_tag="J1Z28_000772"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBA7715629.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(48241..49032)
                     /gene="nanR"
                     /locus_tag="J1Z28_000773"
     CDS             complement(48241..49032)
                     /gene="nanR"
                     /locus_tag="J1Z28_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBA7715630.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        12239 a        13212 c        12188 g        11561 t
ORIGIN      
        1 ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc tgggatttgg
       61 tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccacgcca cattttgtca
      121 acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt gaatactttt
      181 accttctcct gctttccctt aagtgcatta ttttacaaaa aacacactaa actcttcctg
      241 tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat tcagtgagag
      301 cagaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga cgacgattct
      361 caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc tggacaccag
      421 tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata ctgcaaacct
      481 gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg aatacggcgc
      541 ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta ttgccgctat
      601 tcagtgtggc gcatttcata acaccactgg tggactgacc tatttcaaca cgacgccgct
      661 gggccgcgcc gtgaccggca ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat
      721 ctggggtgac ggcagcacct ataaaggaaa cgatatcgaa cgtttctacc gttacggtct
      781 gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact ttattgatga
      841 actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg actacaaaat
      901 gtctgtcgaa aaagcttact ccacggactc caacatgctt ggtgcaacgc atgaagcgaa
      961 ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaagtt
     1021 ttgggatgag agcgtgaaaa tcccggcaga agaagtcaca gtacgctttg agcaaggtca
     1081 tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc tggaagctaa
     1141 ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgaaaacc gtatcatcga
     1201 agcaaaaagc cgcggcattt atgaagcccc tgggatggca ctgctgcaca ttgcgtatga
     1261 acgtttgttg accggtattc acaacgaaga caccattgag cagtatcacg cgcatggtcg
     1321 tcagttgggc cgtctgctgt accagggacg ttggtttgat tcccaggcgc tgatgctgcg
     1381 ggactctctg caacgctggg ttgccagcca gatcactggt gaagttactc tggagctgcg
     1441 ccgcgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct acaaaccaga
     1501 gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta tcggtcaact
     1561 gaccatgcgt aacctggata tcaccgatac ccgcgagaaa cttttcggtt atgccaaaac
     1621 tggcctgctc tcctcctctg ccgcttcagg cgtgccgcag gtggagaatc tggaaaataa
     1681 aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg attatcctct
     1741 gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc cacaacccgt
     1801 gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc cggctttgtt
     1861 acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc
     1921 gttgtgcgat ccacgccatc ccctagaacg gggctatacc agataaacat cgggacatga
     1981 tacgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt cgtagggtcg
     2041 cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt taataattca
     2101 aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca ggcatcctga
     2161 tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc cggttcatgg
     2221 cttccattta aatgaagaac aatcagtttt ttttgctgcg tcttttgctg taacgcttgc
     2281 gataaatgcg gcaacaacaa ttcatcaaat cctctgacat agactgtttc catggcgcgc
     2341 atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga gcttagccag
     2401 aaagtctgaa atcctgcttg attagccata ttaataatgt tgtccgggta attatgaatg
     2461 tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt
     2521 gcaccgctta ttgcttgatt aaacagtttg atctgctttc tttgtgcttc gacttgcggt
     2581 gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga ctcccccaca
     2641 atcaacacgt aggtatcaat acctgtatcc ctgactgata gttgaaaata cggcaccgtg
     2701 tttgcaattg agagtaatct ttgatgctct ttcgctgcta aagcaaaata attgagattg
     2761 aaaaacggcg tataggtagc aaatcgcgac gctagtatat atggactgaa cgcctcttta
     2821 ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga aatgacaatc
     2881 agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt tattattact
     2941 gcaaaaaaaa gaagggataa aaaggcaaaa gcacatagat aagggatata cattcccagc
     3001 attttgacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc atcattgaaa
     3061 gttgtaccaa agatacacca tgaatagagg ctaatactca tatctgccgc aatcagtaac
     3121 acaaatggta cggcaataag caggcgaagc ggaagggatg atatacgttt aacaaccagg
     3181 atggtcagaa cgaagaacag caatcgctga accatatgcg ctccggaaga agcgactaag
     3241 ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt aaaactttta
     3301 acaaatttgt tgaatactgt catccaggaa tccaagttca caaccaatat aaacaattat
     3361 caacaatgtt caattttata tcagacatgt ttttcaactc tgatccatat cagaccgaaa
     3421 cactgttaga ttttttcttt ttaaatgatg cggccattgc gttctcagta attaaataac
     3481 attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta tactgacagg
     3541 ggcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaaat ggagttgaca
     3601 caataaagtg ccaattatgt cagcagaagg gaaaagctta taactaaggc ataccggcac
     3661 catcgtttca aggtaccagc tacgagtaaa acaactggac gagatacaga tacctgacaa
     3721 ccattcctca gactaggacc aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa
     3781 tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc acctcaaggt
     3841 agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc gggatatcgc
     3901 tggtcggctt agccggagct gctggctgag tttgttcggt tttcgccggc gcactcaggt
     3961 tttcccattc gctaccttta ttggttttgt tgctattgat gttacccagc accagactga
     4021 tgatgaagaa taacgttgcc agcagcgctg tcatgcgggt catgaagtta ccagaaccac
     4081 ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata tcagcgcctt
     4141 taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg aaaactacta
     4201 aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa
     4261 ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac taaccaaagc
     4321 gaatgacctt cgcaagggca attttattgc attgtatcaa ctgcggaaaa aaacagcaaa
     4381 accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca gccatttatc
     4441 gttttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca cctgcgcttc
     4501 gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac gcaacagcac
     4561 acgtccacgg ctacccagcg ccgcttcaac ttctgcggtc acagctttga ctgattcatg
     4621 ctccaacgga tcgccgctac ctgcggtgta acgcacatta accagaatct gcgggaacat
     4681 tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgcgccatcg ccgccagcac
     4741 ctgcaagcca gcaacaatgc cgtcaccggt agtggtttta tccagcagga tcacatgacc
     4801 ggagttctct gcaccgatac gccagccttt ctcctgcatt ttttccagta cgtagcggtc
     4861 acccactttc gcgcgcgcaa atggaatacc taactgtttc agcgccagtt caagccccat
     4921 gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa gaccttcacg
     4981 cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt caaccataat
     5041 cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag ccagcacacg
     5101 agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatattta caccgttcgg
     5161 ctcacaaccg atagcgataa cgttcgcccc cagttcacgc agcacgttcg gtgcgatgtg
     5221 ataagtcgca ccgtttgcac aatccaccac aatcgtcagt tcactgaggc taagttcgtt
     5281 cgggaacgtg gctttgcaaa actcgatata acgacccgcg gcatcaacga tacggctggc
     5341 tttacccagt tctgccgaat caacacagct gatctctttt tccatttccg cttcgatggc
     5401 ttcttcaact gcatccggca gcttagtacc gtcaatagaa aagaatttaa tgccgttatc
     5461 gtagaatggg ttatgcgatg cagaaattac aattccagcc tctgcgcgga aggtacgcgt
     5521 cagataagcc acggccggtg ttggcatcgg gccagtgaag agcgcggaaa gaccggcagc
     5581 cgcaagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg tgtctttacc
     5641 aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc aacccagctt
     5701 aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc catcggtacc
     5761 gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg gcttccacca
     5821 cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc gcgccttgca
     5881 ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc cccacgttca
     5941 gcaattgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg ttgaaatggt
     6001 gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta ccgaaaccga
     6061 atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt
     6121 gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcggc gcttcctgca
     6181 tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt tctgcagccg
     6241 cctccagagc accaggttcg gaaagggagc ggatatcgtt aataatgtga gcgccaactt
     6301 tcgctgactc acggatgact tctggtttgg atgtatctac cgagatccag acttcgaagc
     6361 gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca acgctaactt
     6421 ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg ccagcgttga
     6481 tcatcagatt cgcatgtttc accgcatcta acagcgagtt atgcgtgcca ccatccgaaa
     6541 aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg tccagtgaag
     6601 taccctgggc aaagagtttc atgatgttat ccctggtatg aattgataag aaaaaacccc
     6661 ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatacgggaa cttacttgtc
     6721 gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat caaccggacg
     6781 aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt cttcccagcc
     6841 cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg cgtcgatagt
     6901 ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt cggtcagaag
     6961 ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt cgatgatacg
     7021 tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga acacttcacc
     7081 ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact gagtcaccat
     7141 gttacgtgcc aggttggtcg caactttaat gtcgttggac gccccggtag aaacatgttc
     7201 cggtccgtag atgatctctt ccgccagacg accaccgtac agcgtggaga tctgactttc
     7261 cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag tcacacccag
     7321 cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg
     7381 accgataatc gcgtggccag cttcgtggta agccgtcgat tctttctgcg cttccgtcat
     7441 caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct cgaactcaac
     7501 catcgaaaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt tcaccaggtt
     7561 cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg cgtcgatatc
     7621 gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc
     7681 tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcaggg ctgggtcgag
     7741 aacgtccgga cggttagtcg cagcgataac gatgatacct tcgttacctt caaaaccatc
     7801 catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac cgcccaagcc
     7861 tgcgccgcgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc acggcgccgc
     7921 tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga cgaacatttc
     7981 tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg caattgcttt
     8041 cgccagcaac gttttaccgg tacccggagg acctaccatc aggacgcctt tcgggatctt
     8101 accgccgagt ttctggaagc ggctcggctc gcgcaaatac tcaaccagtt cagcaacttc
     8161 ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtg gttttgatct gatcttccgt
     8221 cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc cgccctgcat
     8281 ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc aggagatgaa
     8341 gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga cgttcttggt
     8401 caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat aacggttact
     8461 atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt
     8521 attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact cgctgggccc
     8581 aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta ttaggttttt
     8641 cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca gcgtcaggat
     8701 actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg gtttacgccc
     8761 ggtcgctaca atatacactt cccgcgaacg tgcacgagaa gagtccggct tacgaacttt
     8821 gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt cgccctggaa
     8881 caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt ctagcgccag
     8941 ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca tgtttggtgc
     9001 catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca gtgctttcat
     9061 caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag gatccatagg
     9121 taaaagatcg caagcgatga tgcggccttt gccgccgatt tgggtgacca catattgtga
     9181 ccaaccaccc ggagctgcac caaggtcgac aaccgtcatt cccggtttaa agagtttgtc
     9241 actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc
     9301 ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc tggcagaacg
     9361 cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat
     9421 ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg ttttcaatcc
     9481 caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac ctgaaaggtc
     9541 tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc
     9601 tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa atcgccaccg
     9661 aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta
     9721 atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa cgtaaaatct
     9781 cgctgccacg ctaagattat cctaaagtta cacacattcg ctgtgtaaaa cgaggggttt
     9841 tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc gcatagcggc
     9901 ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct tacaggtatt
     9961 ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc acaacatcat
    10021 cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc aggttttgtt
    10081 taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca gaatcgagat
    10141 tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt ttggtgacat
    10201 caatcacctg cgcgttcgac agcttggctt cgatgtcttt gatacggcct tcgcagaaac
    10261 cctgctgctc acgagctgcg tggtattcgg cgttttcttt caggtcgcca tgctcacgcg
    10321 cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa tccagctctt
    10381 cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt tatacctctt
    10441 gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc ccggcataac
    10501 tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca ggtcagctac
    10561 aattcacatt ttgatagtca ttttaccctg gagttcccga agggtcatcg tttactttat
    10621 agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag attatgcgat
    10681 tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg
    10741 ttgatgggta cattactcaa ctccccgcag gtgccaacct tgccctgatg gtgcaaaaag
    10801 tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgcta cccgccagta
    10861 cgcagaaagt gattactgcg ctggcggcgt tgattcaact cggccctgat tttcgtttta
    10921 ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt gacttagtag
    10981 cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg gtcacgactt
    11041 tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc tccattttcg
    11101 ccagccacga taaagctccc ggctggccat ggaatgacat gacacaatgc tttagcgctc
    11161 cgcctgccgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac agtgccccaa
    11221 aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg atgttcagtc
    11281 aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac
    11341 caggtgacct gaaccgcttt acgctaacgg gatgcttgcc acaacgttct gagccgctcc
    11401 cgttggcttt tgccgtgcag gatggtgcca gctatgctgg ggcaattctg aaagatgagt
    11461 taaaacaggc gggtatcacc tggagcggaa cgctgctgcg ccagactcag gttaacgaac
    11521 ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt aagattatgc
    11581 tgaaaaagtc ggacaacatg attgccgata cggttttccg catgatcggc catgcgcgct
    11641 tcaatgtgcc tggaacatgg cgggccggat cggacgccgt gcgtcagatc ctgcgccagc
    11701 aagccggtgt cgatatcgga aacaccatta ttgccgatgg ttcagggctt tcgcggcata
    11761 acctgattgc ccccgccacc atgatgcagg tgctgcaata tattgcccaa cacgacaatg
    11821 aacttagctt tatctccatg ctgccgctgg cgggctatga cggctctttg cagtaccgtg
    11881 caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg
    11941 gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc
    12001 aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt attccgttag
    12061 tgcgttttga aagccgtttg tataaagata tttatcagaa taattagcct aaagagaaac
    12121 cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc
    12181 gtcgtcttcg tcccagtcgt catcccagtc ctcatcatcc tcttcagcaa tctcttcaag
    12241 ctgctgacgg tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc
    12301 agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac
    12361 gcccagtccg ctcgccgcag agatcagata atatttatct tcccagccca gcgcttcagc
    12421 gatcgctttc gctttctctt cggcttctac cttatccagc agatcgatct tgttgaagac
    12481 taaccaacgc ggtttcgccg ccagatcctg gctgtatttt tccagctcac tgataataat
    12541 acgcgcgttt tcaaccggat cggtgccgtc aatcggatcg atatcgatga ggtgcaacag
    12601 gacgcggcaa cgttccaggt gcttcaggaa gcgaatgccc agacctgcgc cttctgcagc
    12661 gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccatacgcac
    12721 cacgcccagg cttggtacca gagtggtaaa cggataatcc gccactttcg gtttagccgc
    12781 cgataccgca cgaataaagg tcgatttacc cgcgtttggc atccccaaca taccgacgtc
    12841 agccagcagc atcagctcca gcagcagctc acgcttatcg cccggcgtac cgttagtttt
    12901 ctgccgtggt gtacggttaa ctgacgactt gaaacgggta ttgcccagac cgtgccagcc
    12961 gcccttagca accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccggt
    13021 accctggtcg attacacgcg ttcctactgg tactttaatc gtgacgtctt taccgcgctt
    13081 accggtacag tctcggcttg caccgttctg accgcgctct gcacggaaag atttttcaaa
    13141 acgataatcg ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccaccatc
    13201 accgccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaaactcac
    13261 gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat
    13321 tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg
    13381 taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa
    13441 cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag
    13501 tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac
    13561 ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaatgtatt
    13621 cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg
    13681 atctgccaga gggaagagcg cagttgtaca taaagtgtac agtaaaaaca ggatctgcgg
    13741 tgaggccagc cgacgcaata aaaccttttg cgccacgcca taactcaccc aaaccgtcgc
    13801 cgcaccgacc ccaaagataa ctccccaggt gtaatcggtg agctttgtaa atatctcgac
    13861 cagactggtg ttaaaaaaca tcaccaggcc gcttaggagc atcaatgccc ctacaacctg
    13921 agtactgcgc attttctctt tcaggataaa tacgctggca accatcatgc caactggcga
    13981 gagttgccca atcacctgcg aagcggtcgg actcaggtat tgcaaggatg agctgaacag
    14041 gatgaagttc ccaaacagcc cggcagtcgc cactgctaac aaaatcaacc agcgtggctt
    14101 acgaaacacg agtaatggcg gcaaccgctt cttcaccgca agaatggcac caaggccaat
    14161 actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg
    14221 cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc
    14281 aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatatgcgg attaacgtcg
    14341 caccatgcgc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct
    14401 ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt
    14461 tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc
    14521 acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat
    14581 gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga
    14641 atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc
    14701 aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc
    14761 tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg
    14821 tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact
    14881 tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg
    14941 tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt
    15001 tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt
    15061 gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt
    15121 gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca
    15181 ttttttcagt acaatcaccc tatattccta accataaacc ctaagttgcc tttgttcaca
    15241 gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt
    15301 taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg
    15361 tccaactgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc
    15421 cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca
    15481 ttgctgccct gatcgagttt atccacacgg cgactctgct acacgacgac gttgtggatg
    15541 aatcagatat gcgcaggggt aaagctaccg ccaacgccgc atttggcaat gccgccagcg
    15601 tgctagtagg cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggttcac
    15661 tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc
    15721 aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctaca
    15781 gcaagactgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta
    15841 cgccggagga ggagaaaggc ctacaagatt atgggcgcta tctcggcact gctttccagt
    15901 tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa aacgtcggcg
    15961 acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat catggcacac
    16021 caggacaggc acagatgatt cgtaccgcca tcgaacaggg taacggtcgc catcttctgg
    16081 aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgtgccg
    16141 aggaagaagc agacaaagcc atcgcagcgt tacaggtgct cccggacacc ccgtggagag
    16201 aagcacttat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccctca
    16261 tcccgcgcag tgcgcgggat gattccagta aattccataa aacacttatt cagctctata
    16321 actcctgaaa aacagggctg tgtatcttta tatattctga atattcacac tctttacagg
    16381 aactttttag agcaataggc catcaggagt atagtgatgc tcgacagaaa cagaagaagt
    16441 gttctgaatg aaagcgacta acttaaggag tgaggaaaga tggaaagtaa tttcattgac
    16501 tggcatcccg ctgacatcat tgcaggtttg cgcaagaagg gaacgtcaat ggcggcggag
    16561 tctcgcagaa atggtttgag ttcctcaacg ctggcgaacg cattatcacg cccatggccg
    16621 aaaggagaga tgattattgc gaaagccctg ggaactgacc cctgggttat ctggccatca
    16681 cgctaccatg atccgcagac ccatgagttt atcgacagaa cgcagttgat gcgtagctac
    16741 actaaaccga aaaaatgagt ggcctggcgg tagccccgcg aacggggctg ccagctctca
    16801 gacaattact cgcctttcac acgctcaata tttgcaccta aagcgcgtag tttgtcttca
    16861 atgcgttcgt agccacgatc gatgtgataa atacgatcaa ccaccgtcgt cccttccgca
    16921 atacagccag ccagcaccag gcttgctgat gcacgcagat cggttgccat aacctgtgcg
    16981 ccagaaagtt tttcaacacc gtgacaaata acggtattgc tttcgatttc ggcgtgcgcg
    17041 cccatacggc tcagctctgg cacatgcata aagcggtttt caaagaccgt ttcggtgatg
    17101 aatccggtcc cttctgccac caggttcaac agcgtgaact gggcctgcat atcggtcggg
    17161 aatgccggat gcggcgcggt acgtacgtta acagccttcg gacgtttgcc atgcatatcc
    17221 aggctaatcc agtcttcgcc gacttcgatg tccgctccag cgtcacgcag tttcgccagc
    17281 acggcgtcca gagtatctgg ctgcgcgtta cggcagataa ttttgccgcg agagatcgcc
    17341 gccgccacca ggaaagtacc ggtttcgata cgatccggca gcacacgata gacaccgccg
    17401 cctaaacgtt ccacaccttc gatgacgata cgatcggtgc cctgaccgct aattttcgca
    17461 cccagcgtaa tcaggaagtt cgcggtatcg acgatttccg gttcacgcgc tgcgttttca
    17521 ataatcgtgg tgccttccgc aagggttgca gcacacatga tggtcaccgt tgcgccaacg
    17581 ctgactttat ccatcacgat atgtgcgcct ttcaaacgac catcgacgga agctttaacg
    17641 taaccttctt ccagtttgat ggtcgcgcct aattgttcga ggccagaaat gtgtagatca
    17701 accggacgtg cgccgatcgt acaaccgcca ggcagtgaaa cttgcccctg accaaagcgc
    17761 gctaccagcg gccccagcgc ccagatagaa gcacgcatgg ttttaaccag atcgtaaggt
    17821 gcgcagaata cattaacgtc gcgggcatca atatgcacag aaccattacg ttctactttc
    17881 gcacccagct ggcttagcag cttcattgat gtatcgacgt cttttagttt cgggacgttc
    17941 tggatctcta ccggttcttc cgccagtagt gcggcaaaaa ggataggcag agcagcattt
    18001 ttagcgccgg aaattgtgac ttcgccctgg agcttcgttg gcccctgaac acgaaattta
    18061 tccatttagt ttgttctcag ttaacaattc atatccgcta ccggcgaatc gcccatagct
    18121 caaaagccgt tcagtttgcg atcgcgcgcc cactccgcag gggtatacgc tttgatcgac
    18181 acagcatgaa tgcggttatc cgcaatatat tccatcagcg ggccatagac cgtctgctgt
    18241 tttttaaccc gactcatgcc gtcaaacaac tcacccacgg caataacctg aaagtggctg
    18301 ccatcgccgg aaacgtggac ttcctggagg gagagagcgt tcatcaacac gctctgaatt
    18361 tcattatttt ccatgggatc ttcaatcatc agttaataaa tcagcgaaac atcttagagc
    18421 aaagttgcgc tggcataaat aagcaaaaag cctcgctgat aaatcagaca aggctcgact
    18481 tgcaggcagg tttgccggac aggcggttaa cgccatatcc ggcctgaaaa aatttaacga
    18541 ggcagaacat cagcaggcaa attatacaat ttcgccaggg tatacacttt gtcgtttacc
    18601 ccctgaagcg tcacattgtt gccctgcttt ttcgccagat cgataagatg gagcagcagt
    18661 gccagtccac ccgtatccac gcgagacaca cggctaagat cgatgctggt aatccccttc
    18721 accgcttcct cacgtatttc ccaaagcggt agcagaacgt cctgatccag ctctccggat
    18781 aacgccagcg tgtcacccgt ctgcatccag ctcagtgact cgctcattat tttttctctt
    18841 ccagagtgat tttctgttga gaaatcgatt tcagttgcgc agtcaggccg tcgatacctt
    18901 tggtacgcag cagcgttccc cactcgtttt gtttggtggt gatcatactg acgccttcag
    18961 caatcatgtc gtaagcctgc cagttgcccg tctgggagtt tttacgccac tggaagtcca
    19021 gacgcaccgg cgggcggcca ttcgggtcaa taatggtaac gcgaatcggc acaatggttt
    19081 tatcacccag cggctgttct ggcgcaatct gataggtttg accgtgatac atcgccagcg
    19141 cctgaccgta agcctgcttc aggtactcac ggaaagcggc aaagtaagca tcacgttgag
    19201 ctggggtcgc actcttgtaa tactggccca gcaccagcgc accggcgtat ttcacctgta
    19261 cgtatggcaa cagttcctga tcaacaatgg tacgcagata atccgggtta gcccgaattt
    19321 gcggttgctc attcttcagg cgatcgaacg ttttctgcgc cgcctcgtcc atcagcttat
    19381 acggattggt ctggtctgcc gcggttgccg cactcagcgg tgcaatcacc agcaaagcga
    19441 ccatcattaa acgtttaaac atgcgtcggt tctcctgaaa ttatttcgtt gtacccacag
    19501 gttcagtggt ttcattatta cctggcgcag cagctggcgc atcgccacta ttcttattgt
    19561 catcgccttt actaccgtaa aggaactgac caatgagatc ttccagcacc atcgcagact
    19621 tggtgtcctg aattgtatcg ccatccttca ggatagcagt tcccagttcc gggtcttcaa
    19681 aaccgacgtt taatgccaga tattgttccc ccagcaggcc ggaagtacga atgctcagcg
    19741 aactggtatc aggaatgtgg ttataacgtt gttcaatttc cagcgttacg cgcggcagat
    19801 aggttttcgg gtccagcgta atatccgcca cccggcccac aacaacgcca ccaatgctga
    19861 ccggagagcg ggctttcagg ccgccaatgt tatcgaacgt cgcataaagc gtataggtcg
    19921 gttcagtacg tatagacgtc acgttcgctg ccttcaggca aacaaagagc gccgcaagca
    19981 gtgctgctaa taaaaagata cccacccaaa tttcattttt tttcgtttgc atgaactcaa
    20041 ttcccaaaca tcaatgcggt cagcacaaaa tccagcccca gaacagccag agacgagtgg
    20101 acaacggtgc gagtggttgc ccggctaatc ccggcagacg tcgggatggc gtcgtagccg
    20161 ttaaacaacg aaatccacgt caccgtgatg gcgaacacca cgctcttaat cagacagttg
    20221 acgagatcca tacgccagtc gacggcattt tgcattgccg accagaagaa cccactatca
    20281 atgcctttcc aactgacgcc gaccagcgat ccgccccaga tccccacggc gacgaagata
    20341 accgtcaaca gtggtaatga aataactcca gcccagaaac ggggagaaat aacccgacgc
    20401 agcggatcca ccgccatcat ctccatactg gagagttgct ctgtagcgcg catcaggccg
    20461 atttctgcgg ttagcgccga accagcacgc ccggcaaaca acaacgcggc aaccaccggc
    20521 cccagttcac gcagtagcga taacgccacc agcataccca gactggtttc cgcactataa
    20581 gtggtcagaa ccagataacc ttgcagcccc aacaccattc cgatgaacac gccagaaacc
    20641 acaataatca gcatcgacag gacgccgaca ttatagagct ggcgcaccag cagcggtgca
    20701 tgtttgcgaa attccggttt gccgaccagc gcattgaata acattaaccc ggcccgcccg
    20761 aacgttctca gggttttaat ccctttatgt ccgagcgacg ccagcgcatt taacagcatg
    20821 agtggcttaa ctccctggta aaagatcagc gtgataatcg ccggcaggat agcggaacgg
    20881 aacaggcccg tcagctatcc cgtccagaaa ctgacgtacg cgcggatcag gattcgcctg
    20941 caacgcctgg gcactgccat gagcgacaat ttttttgtcc gccaggatcc aggcgtgatc
    21001 cgcaatactt aacacttccg gcacatcgtg agaaaccacc acacaagtca cgcccagcgc
    21061 gctgttcagc tcagaaatca gcttcaccag cacgcccatg gtaatgggat cttgcccaac
    21121 aaagggctca tcaaacatga tgagatccgg ctccagcgca atcgcacgtg ccagcgctgc
    21181 acgccgcgcc atcccaccgg aaagttcaga aggcataagt ttagccgctc cacgcagccc
    21241 cacggcctcc agcttcatca tcaccgtact atgcaacaat ggcgcgggaa gttgggtatg
    21301 ttcccgcagt ggataggcga cgttgtcaaa tacgttcata tcagtgaaca acgccccgga
    21361 ctgaaataac atgctcatcc gtttgcgcac tgtatacagg cgcgaacgag acatcgccgg
    21421 aatattctca ccatcgaaaa ggatctcacc atgatctggt gcgatttgcc cgccaatcag
    21481 acggagtagc gtcgttttac cgatgcccga tggccccatg atcgccgtga tcttccctcg
    21541 cggcacggtc agggaaatat tatcgaagat gcagcgattg ccacgcgtaa aactgacatc
    21601 gcgcatatcg actaaattcg ccacagactg ctccataatt cacccttcgt cttgcgttga
    21661 ttttctaagc atggcgctca atttaacctt gaacccaaca tatttacaga atattacctg
    21721 ccgtggttag cgaaagctgg catttgtttt actttttagc cgcataaagt caaaattaag
    21781 aatctgttac ggctttctga aaatcttcag cggaccggcg agtatacctg aagaaagggc
    21841 gttagatgct tttagctacg gcactgttaa ttgttggttt acttttggtc gtttacagtg
    21901 ccgaccgcct ggtttttgcc gcgtctattc tttgccgaac ctttggcatc ccgccgttga
    21961 tcatcggcat gacggtggtc agtattggta catcgttacc agaaatcatc gtctcgcttg
    22021 ccgcgtcttt gcacgaacaa cgcgatttag ccgttggtac agccctcggc tcaaacatta
    22081 tcaatatatt gctgatcctc ggtctggctg cgctggttcg tccttttacc gtccattctg
    22141 atgttctacg ccgtgaatta cccttaatgt tgttggtcag cgtggtggcc ggttccgtac
    22201 tctatgacgg acaacttagt cgcagcgatg gtatctttct cctctttctg gctgtgctat
    22261 ggctgctgtt cattgttaaa cttgcacgtc aggctgaacg tcaggggact gacagcctga
    22321 ccagagagca gcttgcagag ctgccgcgtg aaggtggatt gcccgtcgca tttttatggc
    22381 tcggcatcgc actgatcatt atgccagtgg ccacgcggat ggtagttgat aacgccacgg
    22441 tgctggcgaa ttactttgcc attagcgagt tgacgatggg tctgacggca attgctatcg
    22501 gaaccagcct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca
    22561 ttgcggtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac
    22621 ccgcgctgat aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga
    22681 tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc
    22741 gtggtgtagg ggtattatta actggcggat ttatcgtatg gctggcgatg ttgtactggt
    22801 tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg
    22861 ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg
    22921 gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt
    22981 aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggca
    23041 gcaacatttg ccagcaccgg tacaccttca tttttcgtcc atcctggcga agccgcgcat
    23101 ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa
    23161 tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc
    23221 atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa
    23281 gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcacaac cgccacgctg
    23341 gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat
    23401 tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat
    23461 attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca
    23521 ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg
    23581 attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt
    23641 cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgcgtgcg cccaggcatt
    23701 ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc
    23761 gatggcgacc atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg
    23821 taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg
    23881 gccctgtcag cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg
    23941 ttgatggcgt actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag
    24001 cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg
    24061 ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca
    24121 ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatctg ctggaaaaac
    24181 tggcgattgc cccggaaaat gtggcttatg tcggcgatga tctcatcgac tggccggtaa
    24241 tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg
    24301 ccgattacgt aacgcgcatt gctggcggtc gtggcgcagt gcgagaagtt tgcgacttat
    24361 tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag
    24421 acgttgggtt atcattgtgc tatcactggg ggttctggtg atgatcggca ttaatatggc
    24481 cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga
    24541 gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca
    24601 acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac
    24661 gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa
    24721 tgaccggatg ctctatttat atggacacgt tgaagtcaat gcactcgtgc cggactctca
    24781 acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc
    24841 tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg
    24901 caacttacgc aacaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca
    24961 aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc
    25021 cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat
    25081 ctcttgatat gcaaggcaac gtggttacct ttaccggcaa tgtcatcgtc acccagggca
    25141 ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag
    25201 aagtgattga cggctacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac
    25261 ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga
    25321 cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc
    25381 tggtgaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc
    25441 tggtgccgtc gcagctgcag gacaaaaaca ataaaggcca gaccccggca cagaagaagg
    25501 gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc
    25561 gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg
    25621 ggccaaacgg tgccggtaag accaccactt tctacatggt tgttggcatt gtgccgcgcg
    25681 atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg
    25741 cgcgccgcgg tatcggctat ctgccgcagg aagcctccat tttccgtcgc ctcagcgttt
    25801 acgataacct gatggcggta ctgcaaattc gtgatgactt gtctgctgaa caacgtgaag
    25861 accgcgcgaa cgagttgatg gaagagtttc acattgagca cctgcgtgac agcatggggc
    25921 agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc
    25981 cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgaca
    26041 ttaaacgcat cattgagcac ctgcgcgaca gcggcctggg cgtactgatc actgaccaca
    26101 acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga
    26161 tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg
    26221 gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat
    26281 tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca
    26341 gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc aggagctaca
    26401 gcaggcgctg gagagtaatc cgctgcttga gcaaatcgat actcatgaag aaatcgacac
    26461 ccgcgaaacg caagacagtg aaacactgga caccgccgac gcgctcgaac aaaaagagat
    26521 gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc
    26581 cggcaccagc ggtgactaca ttgacgacga gctgccggtc tatcagggcg aaacgacaca
    26641 gaccttacag gattacctga tgtggcaggt cgagttgaca ccgttttccg acactgaccg
    26701 cgctattgct acctctatcg tcgatgccgt tgatgacacc ggttatctga ctgtcccgct
    26761 ggaagatatt ctcgaaagta tgggcgatga agagattgac atcgacgaag ttgaagccgt
    26821 ccttaagcgg atccaaaggt ttgatccggt cggtgtggcg gcaaaagatc tgcgtgactg
    26881 cctgctgatc caactctccc aattcgataa gaccacgcca tggctggaag aggccagact
    26941 gatcattagc gatcatctcg atctattagc caatcacgac ttccgcactt taatgcgcgt
    27001 cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc
    27061 gcgccccggg cagtcgatcc agactggcga acctgaatat gtcattccag atgtgctggt
    27121 gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtttgca
    27181 aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg atagccagtt
    27241 tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga
    27301 tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg
    27361 cgaagaatat atgaaaccga tggtactggc tgatatcgcc caggccgtcg agatgcatga
    27421 atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga
    27481 actgaagtat ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac
    27541 ggcgattcgt gcgctggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag
    27601 cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggctc gccgcactgt
    27661 tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg
    27721 acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca
    27781 ccgaggcact gcgcgaattt gttacagcca aatttgccaa acttgagcaa tattttgacc
    27841 gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa
    27901 cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg
    27961 ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa gataaactga
    28021 aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg
    28081 ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag
    28141 gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat
    28201 gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc
    28261 tggcggcgaa acaacttagc ctggcgcctc aggtggtttt tgaagctatc ctgacgcggg
    28321 aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc aaactggaag
    28381 aagatactct gcgcgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg
    28441 ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta
    28501 aaacgcacct gcatactctg tcgctggtag cgaaacgtct ggcggacaaa accatctgcc
    28561 gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag
    28621 gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac
    28681 agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc
    28741 gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg
    28801 atctggctcg aactctggcc gatcgagaga tttctgccgc cgtcagcatt gatgttcgta
    28861 atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct
    28921 caccgcaact actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca
    28981 cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag
    29041 aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgac acctcagaaa
    29101 tgtccgttca cgagctggca gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgtg
    29161 aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt
    29221 acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga
    29281 caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta
    29341 tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta
    29401 gctacctgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag
    29461 agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcggacgc
    29521 tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat
    29581 gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct
    29641 gttacgtaat gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt
    29701 ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc
    29761 actggccgcc gttatcgccc tctttaactc tggttttgat gaagattaat cttcatcact
    29821 ttgacattca aatcccttca agctcccccg gcaataagaa aagcgcgtac tgaaatcgtt
    29881 atcaatcaac atcaattgta catttcgtgc gctttttact ttcagaagaa ccttaagaaa
    29941 accttaagaa gcattgttta gatttcgttt aagttaatcg accatactgg agatcgtcag
    30001 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30061 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtattaa gattgtctac
    30121 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30181 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30241 tgcggaaccg gttatgtcta cgatgtgatt gccgatttcg acggcaagcc ttccattacg
    30301 ctgaccgaat ttgcagaaca atgccgttac gaagaagaca ttgcacaact gcgccagtta
    30361 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagcca
    30421 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tccccgtcgt ctgcgacaac
    30481 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30541 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30601 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30661 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30721 tgacggctac gcacgtagcc cgatggcgcg gagactttga aacgaagtgg attaggtaat
    30781 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30841 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30901 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccggcttcc
    30961 agcccttttc gaacccagct acgcccatcc cataaaaaga gatttttggc tgtctgctga
    31021 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggcttgc
    31081 tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc
    31141 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcgga atgtgccacg
    31201 taacgaaaat tgccatgcag ccaggcgctg acttgccgct cgaccattac tgctgagaaa
    31261 ggaacaggcg caacgctaaa caacgcgatg cccccgcccc agaaaatggc gaggacaacc
    31321 attaaccgca gaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31381 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31441 tctgcgccag catatatgcc ggggtggtga caatcttatt gtcttcatca accacgatat
    31501 catcgacagg acacggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31561 tatcagtacc gatggtcaaa cgtagcggga aatcgaaaat tttcggcagt atcgccggtg
    31621 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31681 attcacggtc aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg
    31741 cgccaaaacc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31801 gacgaatttc accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca
    31861 tcgcttcacc cgttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31921 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31981 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcttcc
    32041 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atgctacgca tcacacattt
    32101 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32161 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32221 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32281 acccacgctt tcagtacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32341 tcaccgacgt tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt
    32401 tgcagatggc gtaaccccac tgaccccgct gcacctttaa ttttatgccc ttcctcaaca
    32461 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32521 attctttcaa acaccgctaa tccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32581 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32641 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32701 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtattc ttgtttgtct
    32761 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgcttc
    32821 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc
    32881 aggtcgtatt cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca
    32941 acgctgttac ctaatttttc cagcacagag cgcgcgacaa tcacgttcag ttcaatgtct
    33001 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatca
    33061 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33121 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33181 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33241 gcgaagattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33301 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgagtgaat
    33361 ttgacggcgt tactgatgag gttccacaag atctgccgta aacgcgtccc gtcggtaatg
    33421 acctgatgcg gtaatggcag cgtcggctcc agattaaagc gcaatccttt ttgttgcgcc
    33481 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33541 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt gaagatattc
    33601 cccagcgtga cagccgaaac atggatggtt ttgagatatt tttcctgctc ggcggtgagt
    33661 tcagtatcca gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg
    33721 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga
    33781 taccgcttac gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg
    33841 cggtcgtagt acggtacttt acggatttca aagcaggctt tgcgtccgtc gggataatcc
    33901 agccactgct cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33961 actttcgcag cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac cagttgtttt
    34021 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34081 ttacgataaa aaaccaggtc gggtgaagcg tcaaggaagg aacgtaagaa tgaggattgc
    34141 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34201 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34261 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34321 tgcaccagtc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34381 aagtagaccg cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg
    34441 ctttcgacct ggccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34501 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcagatca
    34561 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34621 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34681 cctcaatggc ctacgccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34741 catgttcatt cagataacga tccagcccga ccattcccgt ccatcgattt tcgcaccaca
    34801 gcggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34861 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaattccgt
    34921 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcactata tgcagcggat
    34981 gcagctttat gccgtccacg ccggtttcaa ccactcgctc cagcgtttgc aaacattcag
    35041 cctgcccttc gccaggcagg ccaacaatta agtgagaaca caccttcagc ccacgctcac
    35101 gcgccagctg cgtcgtgcgc tgatagcagg caaaatcatg accgcgattg atgcgatgca
    35161 gtgttttgtt gtgggcggtt tgtagcccca gctccagcca cacttcgtag ccctggtcct
    35221 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac
    35281 acaaaccgac aatattggcc tggctcaccg cctgctgata catcgaacgc agcacctgaa
    35341 cttccgcaaa ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa
    35401 ctaaattcgc ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg
    35461 caaacgaggc aacattacag aatgtgcagc cgccacgccc gatggtaccg tcacggttag
    35521 ggcagctaaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35581 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35641 gaaaagaggc caaagcctgc catttttagc ccaattcggc gatgacctgg atcaatcgtc
    35701 ccggcctgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcacaatca
    35761 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35821 aaaacagtag catgaaacgt tattaccaat taaggcagta taaaatgctg gttttctcgt
    35881 cagttcaagg caggataagg gttaacacgc ctttatgaca gtcaggtatt ggctgtttct
    35941 ctaacgacgc cccttttagc cttaaataga aaatccaatt taatttcaga taattaataa
    36001 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36061 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36121 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36181 gggcgaatgc gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct tactgtgccc
    36241 gtgcgcagcg gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36301 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36361 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36421 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36481 gatcgttttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgcc
    36541 gtcgggatgc tcttcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36601 gaagaagagc tgcaacgcga aacgttgtcg attgtgggct ggcgtgatgt ccccactaac
    36661 gaaggcgtgc tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg
    36721 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36781 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg
    36841 gtcaatattt ataaaggtct gtgtatgccg gcggatctgc cgcgcttcta cctggacctc
    36901 gctgacctgc gtctggaatc ggccatctgc ctgtttcacc agcgcttttc cactaacacc
    36961 gtaccgcgct ggccgctggc gcagccattc cgctacctcg cgcataacgg tgaaatcaac
    37021 accatcaccg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg
    37081 atccctgacc tgcacgacgc cgcgccgttc gtcaacgaaa ccggctctga ctccagttcg
    37141 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg tgccatgcgt
    37201 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37261 tttgacttta actccatgca tatggagccg tgggatggcc cggcaggcat cgtgatgtct
    37321 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37381 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttatcagcct
    37441 gacgaagtgg tcgaaaaagg ccgcgtgggg ccaggcgaac tgatggttat cgacacccgc
    37501 agtgggcgta ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat
    37561 aaagagtgga tggagaaaaa cgtccgccgt ctggtaccgt ttgaagatct gcccgatgaa
    37621 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acaatttaac
    37681 tacagcgcgg aagagctgga ttcggtgatc cgcgtactgg gtgaaaacgg tcaggaagcg
    37741 gtcggttcga tgggcgacga caccccattc gccgtgctct ccagccagcc gcgcattatt
    37801 tacgactact tccgccagca gtttgctcag gtaaccaacc cgccaatcga cccgctgcgc
    37861 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttctgcgaa
    37921 gcagaaggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ttccgatttc
    37981 aaacagctca cgacgatgaa agaggatcac taccgcgcag atacgctgga tatcaccttt
    38041 gacgtcacta aaaccacgct cgaagcgacg gtgaaagaac tgtgtgacaa agccgaaaaa
    38101 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38161 ctgccggttc cggctccgat ggcggttggc gcgatccaga cccgtcttgt cgagcaaagc
    38221 ctgcgttgcg atgccaacat catcgtagaa accgccagcg cccgtgatcc gcaccacttc
    38281 gccgtgttgc tgggtttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38341 ggccgtctgg tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactat
    38401 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38461 gcctcttatc gctgctcgaa actgtttgaa gcggtcggtc tgcacgatga tgtcgtgggc
    38521 ctgtgcttcc agggggcggt tagccgcatt gggggggcga gctttgaaga cttccagcag
    38581 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38641 ctgctgaaat acgtgcacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38701 ctgcaacagg cggtacagag cggcgagtat agcgactatc aggaatacgc gaagctggtt
    38761 aatgagcgtc cggcaaccac gctacgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38821 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgccgcg
    38881 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38941 atcggcggta actcaaactc cggcgaaggc ggtgaagacc cggcgcgcta tggcactaac
    39001 aaagtgtcgc gcatcaagca ggtggcgtcc ggtcgctttg gggttactcc ggcgtatctg
    39061 gtcaatgccg acgtcattca gataaaagtc gcccagggcg cgaagccggg tgaaggtggt
    39121 cagttaccgg gtgataaagt cactccttac attgccaaac tgcgctattc ggtgccgggc
    39181 gtgacgctga tctccccgcc gccgcaccac gatatctact ctattgagga tttagcgcag
    39241 ctgattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtgtcc
    39301 gaaccgggcg tcggcaccat cgcgaccggc gtggcgaaag cttatgcgga cttgatcacc
    39361 atcgcaggct atgacggcgg caccggcgca agtccgcttt catcggtgaa atacgcaggc
    39421 tgtccgtggg agctggggct tgttgaaacc caacaggcgc tggttgccaa cggcttgcgt
    39481 cacaagatcc gtttgcaggt cgatggcggc ctgaaaacgg gtgccgatat catcaaagca
    39541 gcgattcttg gcgcggaaag ttttggcttc ggcactggcc cgatggtagc gcttggctgt
    39601 aaatatctgc gaatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac
    39661 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgactaatta ctttgagttt
    39721 atcgcacgtg aaacccgcga gctgatggca cagctggggg taacgcgtct ggtggatctg
    39781 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39841 gcgctgtcga agctgctgga aactgccgag ccacatgcag gtaaggcact ctactgcacc
    39901 gaaaaaaacc cgccgtttga taacggccta ctgaacgcgc agttgctgca acaggcaaaa
    39961 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa caccgaccgt
    40021 tctgtcggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggttggct
    40081 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagcttcgg cgtgtggaac
    40141 gcgggcggcg tggaactgta cctgaccggt gatgccaacg actatgtcgg taaaggcatg
    40201 gcgggtggct taatcgccat tcgtcctccg gttggctcag ccttccgcag ccatgaagcg
    40261 agcattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40321 cgcgcgggtg aacgtttcgg cgtgcgtaac tccggtgcta tcaccgtggt agaaggcatt
    40381 ggcgacaacg gctgtgaata tatgacgggt ggtatcgttt gtattctggg taaaaccggc
    40441 gttaacttcg gcgcgggcat gactggcggc ttcgcttacg ttctcgatga aagcggcgat
    40501 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40561 catgaagagc atctgcgtgg tcttatcacc gagcatgttc agcataccgg ttcccagcgt
    40621 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40681 aagtccagtg atgtaaaagc actgctgggt caccgtagtc gtagcgcagc agagttgcgc
    40741 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40801 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40861 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40921 aacccatact gcgagtggaa atgcccggta cacaactaca tcccaaactg gctgaagctc
    40981 gccaatgagg ggcgtatttt tgaagcggcg gaactgtcgc accagacaaa caccctgccg
    41041 gaagtttgcg gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac
    41101 gatgagttcg gtgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41161 gagatgggct ggcgtccgga tatgtccggc gtgaaacaga ccgataaaaa agtggcgatt
    41221 atcggcgcag gtccggcggg tctggcgtgc gcggatgtcc tgacgcgcaa cggcgtaaaa
    41281 gcgatcgtct tcgaccgtca cccggaaatt ggcggcttgc tgaccttcgg tattccggct
    41341 ttcaagctgg aaaaagaggt aatgacgcgc cgccgtgaaa tcttcaccgg catgggtatt
    41401 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41461 tacgatgccg tgttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41521 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41581 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41641 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg ccagggggca
    41701 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41761 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc gctgggtata
    41821 gaagtgaatg gcaacggcaa agtcagcggc gtaaaaatgg tgcgcacaga aatgggcgaa
    41881 ccggatgcca aaggccgtcg ccgcgcggag attgtggcag gttccgaaca tatcgttccg
    41941 gcagatgcgg tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    42001 cacagcgtcg agctggattc gcaagggcgc attatcgccc cggaaggcaa cgacaacgct
    42061 ttccagacca gcaacccgaa aatctttgct ggcggtgata tcgtccgagg ttccgatctg
    42121 gtggtgaccg ctattgccga aggtcgtaag gcggcagacg gcattatgaa ctggctggaa
    42181 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42241 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42301 gttaccggta gatgcccggc gtatagctga ttccattgtt caacgcagcg ccatataaag
    42361 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42421 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42481 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42541 gccagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42601 tataagcgtg ggttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42661 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42721 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaatctttc
    42781 cccctgccct ggtccactta cgtccgtttg ctttctgtta aaaacgctga cgctcgtagc
    42841 ttttatgaaa aagagacgct ccgctgtggc tggtctgttc gccagctaga gcggcaaatt
    42901 gcgacccaat tttatgagcg aacactactg tcacatgaca aatcagccat gctgcaacaa
    42961 cacgctccta ccgagacgca tattcttccg cagcaggcga tacgcgatcc ctttgtgctc
    43021 gaatttctgg aattgaaaga tgaatattca gaatctgatt tcgaggaggc gctgatcaac
    43081 cacctgatgg atttcatgct ggaacttggg gatgattttg cctttgttgg tcggcagcga
    43141 aggttacgca ttgatgacaa ctggtttcgt gtcgatctgc tgtttttcca ccgccgttta
    43201 cgctgcctgc taatcgtcga tctaaaagtg ggcaaattca gctatagcga tgccggacag
    43261 atgaatatgt atctcaacta cgccaaagag cactggacgc taccggatga aaatccgccc
    43321 atcggtctga ttctctgtgc agagaaagga gccggcgaag cgcattatgc actggctggt
    43381 ttgcctaaca ccgttctggc gagcgaatat aagatgcaac tacctgatga aaaacgactc
    43441 gcggatgaac tcgttcgaac acaggcggtg ctagaggaag gctatagacg ccgttaattt
    43501 cagcggtttc gaattctgca gacagtgtct gcagaatctt taaaacgaac taagttttaa
    43561 gccattaaat cagccttaac cttcactacc acctttttaa tctcaccagg ttcgccaaca
    43621 acgcatcccg gtttatgcgg ttctcctggc ataaacacgg cgaacattcc cggtttcagg
    43681 atgatggctt gctcgttttc aatggcgctg caaagctgat aatcatcttc atggtggaac
    43741 tcttcacact gacgcgcagt gcctgccatg ccaaacagaa tccgttcctc accgtttaat
    43801 aacagctgga tatcaatgta ttgttcgtgc aattcggctt ttttctcaac gggcgattga
    43861 gtattaaatg tcatgacatt cataaagata ttgtcgccct gtaattcgta acggccaggc
    43921 gctttttctt gcggtctggc agctaatgcc aacgttaacg cgtcctgtaa cgcaggatgt
    43981 aatccggcag acggtaatga ctgtacttca cccatcatca taatatttct ccctgggcca
    44041 acagcgcagc cccaagtaaa cctgcatcgt ggcggtagtg cgccgccagt aaatcaacat
    44101 gaaatgccgc tggctcctgc gccagatacg tttccaccag cgccagatac ccttctgcca
    44161 gaccaacgct gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga
    44221 tcagccttgc aagcgtacgt gcggagcggt gaatcagttg ctgcgcctgc tcatcaccct
    44281 gcccggcgcg cgtgaaaata gttttcgcat tcgcaccagc caactccccc tgcgctgccg
    44341 ttgcaatgcc gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga
    44401 ctggaccatg cggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg
    44461 tacgcagttt accgccgctc actacaccgc cgccaacgcc ggtggaaaca gtgataaaca
    44521 ccatatcggt tatatcgcca tccagcgcct gatactccgc ccatgctgcg gcctgcgcat
    44581 cattaatggc aatggtcggc aaattggtaa gttgctccag cgttttgact aacggaaagt
    44641 gtaacaatcc accgagatta tgcggattca gcgccagcaa gctgccgtcg cgaataattc
    44701 cggttgaagc gatggcaacc cgctgcgcat gtgcttgcaa cggagagact aatgcggata
    44761 aggcatcacg cagcgcctgc ggagtctggc tggcaggcgt aggaagttct cggcgatcgc
    44821 ggatctgccc gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg
    44881 ccagtgtggt catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc
    44941 aagacgcgta atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatccgc
    45001 cgcctgagca ggcgtgttgt aacgcccttc ggcaatcacc cggcatccgg cgtcactcaa
    45061 cgttttcacc agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag
    45121 cgtagtgcca ataatttcgg ctcccagctt ttggcatgcc aggccgtctt ccggcgttga
    45181 gcagtcggtc atcgccagta aaccgtgatg gtgaatacgt gccagcagcg tttcaacaag
    45241 taccggacgc ggtcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag
    45301 cgcatcaaca tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgctt
    45361 cacaatccca ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc
    45421 ttcaatacga atggcgaccg cgcccgcctg ctctgccgct aatgccatgg cagcgacgat
    45481 ttcgggttta tcgagcgggc tgtccggaac cggctggcag gagacaatca ggccaccgtt
    45541 agcagcgatt ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg
    45601 cccggtacag agaccgggca acaggattaa cttttggttt tgactaaatt gtttttggcg
    45661 ctgccaaatg gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat
    45721 gcttccgggc gcagccaacg ctgaacacga gaaggcatat ccagaccaat cagcaggatc
    45781 accacaaatg tcagactgaa cgagagcgat gccagcgcag tacccagatc cagacgttga
    45841 gcgatcaacg cgccgatgat tggggccagt gcaccgccca atgcgccaac gttgtaggta
    45901 aagcccagcc ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag
    45961 atcccggcga tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag
    46021 acgtttgcgc cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcagacta
    46081 caaacgtacg ctttgcgggt tcccagccag tcaccgagga agcccccgac gcagcacccc
    46141 accgccgcgc caaagccact aaagaacagc acattggcga cagtatgcgg gtcataagcc
    46201 agatcggttt tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagcg
    46261 aacaacacga ccaccatcag cattacgccc gttggccagc gtttgcccgt actctgcacc
    46321 ataaagctga taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt
    46381 tgcaggttac cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata
    46441 ttggcaatgc gatgttcacc acggtagaga atgtctacca tcgtgcgtac tggtgcttta
    46501 cccccgtgtt tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca
    46561 aagatgattg gcaaaatgcc gataaagaac agcgcacgcc agccccagac cggaaccacc
    46621 agactataga cctgagcggc aacgacggcc cccactgaga agcctgaaat cagaaaacca
    46681 ctggctttgt tacgcagatg ctttggccag ctttcaatga cataggtggc gctggaaccg
    46741 tattcacccg ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct
    46801 ggcgcaaagc cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc
    46861 agacgacgcc cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag
    46921 ataaaggctg cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt
    46981 acttcggtga gtactagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac
    47041 caggcagcgg aaaatgcccg ccattgtgcg cggttaagat ggcgatacca cgggatattc
    47101 tgggttgtag tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg
    47161 aaaattgtag ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa
    47221 acaactcacc cgcgctcttg catcaactgc tgggccagcg ccttcagttc tggcagatat
    47281 ttttcatcta ccggaccaaa cggtttgcgg cacagcggca cagaaacgac atccatataa
    47341 tgcaggacag ttttcaggcc gcggaatacg ccagttttga tcagtaaatc aatgactttg
    47401 ttgcactcag tttgcagttt ctgcgcggtc tggatatcgc cttctttcag cgccttcacg
    47461 atcccctgat aacgccagcc catgatgttg taagtgctgc cgataccgcc gtctgcgccc
    47521 gccagaagac cagaggcgaa gatttcgtcg taaccgttat agagcaccag atcaggatgt
    47581 tcacgacgga tctgctccat ctgatagaga tcgccagagg tctgtttcag cgcacctaca
    47641 ccaggcagcg taaccagggt gttgatctga tccagggtca gttttacccc gctcagggct
    47701 ggaatgttgt acaccaccat cggcaaccca tccgccgaat caataattgc ccgatagtga
    47761 tcgcagtgtt cttcaaagct gaaaggatag tagaacggcg tgacggcgga gacggcatcg
    47821 aagccataac gtttagccga tgccgcaagt tgttggcttt cggcggtgct gacgcaaccg
    47881 acgtgggcga tgagtttaat cttacctttc gcctcttcag cgacgatttc cagtacctgt
    47941 tcacgctctg aaaggctctg tacaaaggct tcgcctgtcg aaccacccac gtacaaaccg
    48001 tcgatgccct gctgaatatt gaactgaacc aggcggcgca gactcgcttt atccagtgct
    48061 tgttgttggt caaaaggagt caggagtgca gccattacgc cacgtaaatt cgttgccata
    48121 aatacctctg aagtgatgct tgtctgataa acgatatacc tttatacctg ttataccaga
    48181 tcaattaagc aacaccccat acagaaagct tataatgcga tctgcttcac taaagtggca
    48241 ttatttcttt ttgttggtgg tctgaccgaa ggcgtgccag gtagcagaga cgctgttgag
    48301 atgcgattgc aacgcgcgat cggcttcgtc aggatcatga cggcggatcg cgtcaacgat
    48361 cgcaatatgc tgttgatagc taacgttgtt atgctcatgc agagcctgat cggccacggt
    48421 tggacgtgcg gcgattagcc agtcgagcag ggcaacgtgg atcgccatga agattgggtt
    48481 accagggatc tccgccagaa cgcggtggaa atcaacgtct gagcgaatga atgccgcgtt
    48541 gttatccagc gactgactgt tgatttccag tgcttttgcc agcaaatcga tttgctcatc
    48601 ggtggcgtgt tcagccgcat agcgcaccag gctggattca aagaacagac gtaattgttc
    48661 gaaatgggca atcccaccag gatgagaaag gaaatctttc gccatgccgg aaagctcgcc
    48721 gatgatggtg tccgcagaag gacgtgagac gcgggcacgt tcgccgttgt ttatttgcac
    48781 cagacctttg cgttttaacg ctgccagcgc ttcgcgcacc gaaggacgcc cgacgttaaa
    48841 gaacgccatc agttcgcgtt cagacggtaa ttgttcacct tcgccaaatt cacgacggcg
    48901 gatcatctgt tccagctctt cttccaccat ttcggagagt tttttacgcg ccagcgggcg
    48961 gctacgcaag ttgcgaccaa ttgcaggtga agaatcttcg gtttgcgaat caaatgcgtt
    49021 cataaggccc attctgtaag gtcagtgtga ttaacatcat cagtgacatc ctatcacagg
    49081 attgaaagta ggggaaaatg gcagggtttt ctctttgtgc ctcatcatta ccataattaa
    49141 cggaataatt aactattgcg aaaaattaat gtaacgcaga taaaaacatc ccgattaaat
//
LOCUS       DADSZD010000015        47640 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.015, whole genome shotgun sequence.
ACCESSION   DADSZD010000015 DADSZD010000000
VERSION     DADSZD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.015"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            156..1373
                     /locus_tag="J1Z28_000774"
     CDS             156..1373
                     /locus_tag="J1Z28_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBA7715631.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1457..2056
                     /gene="yfbR"
                     /locus_tag="J1Z28_000775"
     CDS             1457..2056
                     /gene="yfbR"
                     /locus_tag="J1Z28_000775"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBA7715632.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2115..3947)
                     /locus_tag="J1Z28_000776"
     CDS             complement(2115..3947)
                     /locus_tag="J1Z28_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBA7715633.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4034..4684)
                     /gene="hxpA"
                     /locus_tag="J1Z28_000777"
     CDS             complement(4034..4684)
                     /gene="hxpA"
                     /locus_tag="J1Z28_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HpxA"
                     /protein_id="HBA7715634.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4695..5189)
                     /locus_tag="J1Z28_000778"
     CDS             complement(4695..5189)
                     /locus_tag="J1Z28_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBA7715635.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5271..5726)
                     /locus_tag="J1Z28_000779"
     CDS             complement(5271..5726)
                     /locus_tag="J1Z28_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBA7715636.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6064..7266
                     /gene="ackA"
                     /locus_tag="J1Z28_000780"
     CDS             6064..7266
                     /gene="ackA"
                     /locus_tag="J1Z28_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBA7715637.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7341..9485
                     /gene="pta"
                     /locus_tag="J1Z28_000781"
     CDS             7341..9485
                     /gene="pta"
                     /locus_tag="J1Z28_000781"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA7715638.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9675..11195
                     /gene="yfcC"
                     /locus_tag="J1Z28_000782"
     CDS             9675..11195
                     /gene="yfcC"
                     /locus_tag="J1Z28_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBA7715639.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11228..11770)
                     /gene="yfcD"
                     /locus_tag="J1Z28_000783"
     CDS             complement(11228..11770)
                     /gene="yfcD"
                     /locus_tag="J1Z28_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBA7715640.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11828..12379)
                     /gene="yfcE"
                     /locus_tag="J1Z28_000784"
     CDS             complement(11828..12379)
                     /gene="yfcE"
                     /locus_tag="J1Z28_000784"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBA7715641.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12435..13079)
                     /gene="yfcF"
                     /locus_tag="J1Z28_000785"
     CDS             complement(12435..13079)
                     /gene="yfcF"
                     /locus_tag="J1Z28_000785"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBA7715642.1"
                     /translation="MSKTAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPAWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13215..13862
                     /gene="yfcG"
                     /locus_tag="J1Z28_000786"
     CDS             13215..13862
                     /gene="yfcG"
                     /locus_tag="J1Z28_000786"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBA7715643.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHATPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            13919..14281
                     /gene="folX"
                     /locus_tag="J1Z28_000787"
     CDS             13919..14281
                     /gene="folX"
                     /locus_tag="J1Z28_000787"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBA7715644.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14302..15195
                     /locus_tag="J1Z28_000788"
     CDS             14302..15195
                     /locus_tag="J1Z28_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family protein"
                     /protein_id="HBA7715645.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQNDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRFGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15354..16127)
                     /gene="hisP"
                     /locus_tag="J1Z28_000789"
     CDS             complement(15354..16127)
                     /gene="hisP"
                     /locus_tag="J1Z28_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBA7715646.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(16135..16851)
                     /locus_tag="J1Z28_000790"
     CDS             complement(16135..16851)
                     /locus_tag="J1Z28_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715647.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(16848..17534)
                     /locus_tag="J1Z28_000791"
     CDS             complement(16848..17534)
                     /locus_tag="J1Z28_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311219.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBA7715648.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(17624..18406)
                     /gene="hisJ"
                     /locus_tag="J1Z28_000792"
     CDS             complement(17624..18406)
                     /gene="hisJ"
                     /locus_tag="J1Z28_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBA7715649.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(18627..19409)
                     /gene="argT"
                     /locus_tag="J1Z28_000793"
     CDS             complement(18627..19409)
                     /gene="argT"
                     /locus_tag="J1Z28_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBA7715650.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(19675..20244)
                     /locus_tag="J1Z28_000794"
     CDS             complement(19675..20244)
                     /locus_tag="J1Z28_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBA7715651.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(20339..21856)
                     /gene="purF"
                     /locus_tag="J1Z28_000795"
     CDS             complement(20339..21856)
                     /gene="purF"
                     /locus_tag="J1Z28_000795"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBA7715652.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(21893..22381)
                     /gene="cvpA"
                     /locus_tag="J1Z28_000796"
     CDS             complement(21893..22381)
                     /gene="cvpA"
                     /locus_tag="J1Z28_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBA7715653.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(22731..23393)
                     /gene="dedD"
                     /locus_tag="J1Z28_000797"
     CDS             complement(22731..23393)
                     /gene="dedD"
                     /locus_tag="J1Z28_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBA7715654.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKLVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(23383..24651)
                     /gene="folC"
                     /locus_tag="J1Z28_000798"
     CDS             complement(23383..24651)
                     /gene="folC"
                     /locus_tag="J1Z28_000798"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBA7715655.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(24721..25635)
                     /gene="accD"
                     /locus_tag="J1Z28_000799"
     CDS             complement(24721..25635)
                     /gene="accD"
                     /locus_tag="J1Z28_000799"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBA7715656.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(25791..26450)
                     /locus_tag="J1Z28_000800"
     CDS             complement(25791..26450)
                     /locus_tag="J1Z28_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7715657.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(26533..27345)
                     /gene="truA"
                     /locus_tag="J1Z28_000801"
     CDS             complement(26533..27345)
                     /gene="truA"
                     /locus_tag="J1Z28_000801"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBA7715658.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(27345..28358)
                     /locus_tag="J1Z28_000802"
     CDS             complement(27345..28358)
                     /locus_tag="J1Z28_000802"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA7715659.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATATWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(28424..29560)
                     /gene="pdxB"
                     /locus_tag="J1Z28_000803"
     CDS             complement(28424..29560)
                     /gene="pdxB"
                     /locus_tag="J1Z28_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBA7715660.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLSDADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLSEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            29659..30654
                     /locus_tag="J1Z28_000804"
     CDS             29659..30654
                     /locus_tag="J1Z28_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBA7715661.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSHHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSTYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(30651..31829)
                     /locus_tag="J1Z28_000805"
     CDS             complement(30651..31829)
                     /locus_tag="J1Z28_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HBA7715662.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(32061..33281)
                     /gene="fabB"
                     /locus_tag="J1Z28_000806"
     CDS             complement(32061..33281)
                     /gene="fabB"
                     /locus_tag="J1Z28_000806"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBA7715663.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            33440..35446
                     /gene="mnmC"
                     /locus_tag="J1Z28_000807"
     CDS             33440..35446
                     /gene="mnmC"
                     /locus_tag="J1Z28_000807"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBA7715664.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNHLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPLPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGSTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDLLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLKLAQQQGLQIHYQYQLQDLSRKDDGWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSDEDQQQNRQRLIDCFPHAQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(35567..35845)
                     /locus_tag="J1Z28_000808"
     CDS             complement(35567..35845)
                     /locus_tag="J1Z28_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBA7715665.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(35879..36427)
                     /locus_tag="J1Z28_000809"
     CDS             complement(35879..36427)
                     /locus_tag="J1Z28_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBA7715666.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALS"
     gene            complement(36427..37236)
                     /locus_tag="J1Z28_000810"
     CDS             complement(36427..37236)
                     /locus_tag="J1Z28_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBA7715667.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(37236..38060)
                     /gene="mepA"
                     /locus_tag="J1Z28_000811"
     CDS             complement(37236..38060)
                     /gene="mepA"
                     /locus_tag="J1Z28_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBA7715668.1"
                     /translation="MNKTAIALLTLLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(38064..39149)
                     /gene="aroC"
                     /locus_tag="J1Z28_000812"
     CDS             complement(38064..39149)
                     /gene="aroC"
                     /locus_tag="J1Z28_000812"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBA7715669.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQKIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(39184..40116)
                     /gene="prmB"
                     /locus_tag="J1Z28_000813"
     CDS             complement(39184..40116)
                     /gene="prmB"
                     /locus_tag="J1Z28_000813"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBA7715670.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            40282..40833
                     /gene="smrB"
                     /locus_tag="J1Z28_000814"
     CDS             40282..40833
                     /gene="smrB"
                     /locus_tag="J1Z28_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBA7715671.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPELP"
     gene            complement(41157..41999)
                     /locus_tag="J1Z28_000815"
     CDS             complement(41157..41999)
                     /locus_tag="J1Z28_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBA7715672.1"
                     /translation="MKIFRWLCCLVMLTCAAESLAAKKIATLVTPVAYSGYLTQIRYY
                     LEILTPEGVEYGVYKQHSIVLQAENLDLVSWTGSTAAPTLKMEELNDASKSTCVGLDA
                     YDARSSKSDWICYNASLGVYYDGEVHGCPWLISTYTDSNISSVDGVGLYIGPKVHNSS
                     CPVSVAAYDISWSEDYVVHSKVLSLQSTGGTIEKTLPTFLMESGKLCDGSVMDDRGAY
                     CRFVAQMITFSTSGCDSSKVTVIPNQHPITDKQLHDMVVRVDTSSRQPIDSTCRFQYT
                     LNEL"
     gene            complement(42001..42528)
                     /locus_tag="J1Z28_000816"
     CDS             complement(42001..42528)
                     /locus_tag="J1Z28_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7715673.1"
                     /translation="MNLKRISHCAAALAAVLLTPHVQAADNLHFYGNLLSKSCTLVVQ
                     GESLAEVHFPTVSRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDASSGAQGVGIGMEKTDGTAVPINNTSGATFLLSNGNNNLNFNAWLQAKSGREVT
                     MGDFTASLTATFEYI"
     gene            complement(42525..43004)
                     /locus_tag="J1Z28_000817"
     CDS             complement(42525..43004)
                     /locus_tag="J1Z28_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7715674.1"
                     /translation="MKRLAWCLIYGFAGLAQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSDGTFSAYATLQVDYQ"
     gene            complement(43001..43504)
                     /locus_tag="J1Z28_000818"
     CDS             complement(43001..43504)
                     /locus_tag="J1Z28_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7715675.1"
                     /translation="MKAFFLTRSAALLLACGAIVSPVMAGGKIVNLTLRVLVDGPPPC
                     SVKGSAVEFGNVIIKSIDGTNYRQPVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     AGISGFGIRIQNAADHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            complement(43521..44273)
                     /locus_tag="J1Z28_000819"
     CDS             complement(43521..44273)
                     /locus_tag="J1Z28_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7715676.1"
                     /translation="MSYKLSCPMLVSTALMALLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKITSPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRRPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKLASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(44293..46941)
                     /locus_tag="J1Z28_000820"
     CDS             complement(44293..46941)
                     /locus_tag="J1Z28_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA7715677.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFDPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKNWDWSRYYAWRALPSLKAKLSLGEDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWKHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNADEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            complement(47022..47588)
                     /locus_tag="J1Z28_000821"
     CDS             complement(47022..47588)
                     /locus_tag="J1Z28_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7715678.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANTDNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIMMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT        11500 a        12974 c        12000 g        11166 t
ORIGIN      
        1 ttgccattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
       61 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
      121 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
      181 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
      241 aagaagaagg taacaaggta ctgaaactga acatcggcaa ccctgccccg ttcggttttg
      301 acgcgccaga tgaaattctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
      361 gcgattccaa aggtctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
      421 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
      481 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcacctgatt
      541 acccactatg gaccgcagcg gtttcgctct ccagcggtaa agcggtgcat tatctttgcg
      601 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
      661 cgcgtgggat cgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
      721 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
      781 tttatgacaa aattctctac gacgacgctg agcatcactc cattgcgccg ctggcacctg
      841 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
      901 aggggtggat ggtattgaac gggccgaaaa aacacgccaa aggttacatc gaaggtctgg
      961 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
     1021 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggaggtcgt ctttatgagc
     1081 agcgtaaccg cgcgtgggaa ttgatcaacg atattccggg cgtttcctgc gtgaaacctc
     1141 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt cacgacgatc
     1201 agaaaatggt gctggatttc ctgttgcagg aaaaagtcct gttggtgcaa gggacggcat
     1261 tcaactggcc atggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
     1321 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
     1381 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
     1441 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
     1501 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
     1561 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcgtaaat ttggcggtaa
     1621 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcttcag aagtgctcac
     1681 cggagatctc cctactccgg tgaaatactt caattcgcaa atcgctcagg aatacaaggc
     1741 tattgaaaaa atcgctcagc aaaaactggt cgatatggtt ccggaagagc tgcaggatat
     1801 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
     1861 ggcagatgca ctgtgtgcat atctgaaatg tctggaagag ctcgcggccg gaaataatga
     1921 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgccgca gccaggagat
     1981 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
     2041 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc accggatgcg
     2101 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
     2161 acgataatgg tgaacggtac ccccaactta acaaagtcac tgaagctgta attccccgga
     2221 cctaaaacca gtgtgttaac aggtgaagat atcggtgtca taaaggcggc ggatgctgcc
     2281 atcgccacga ccatcgcaaa tggataaggc gacacaccca tcgttttggc agcagccagc
     2341 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaatagccc aataaccgcc
     2401 gacaagacaa acaaacaacc cagcatcata tgtggcccgt aaccgccgcc aatgtccatc
     2461 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaatggcatc
     2521 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
     2581 atacagcgga atttccccat cagcaggcag gcgataatag cggcaacagg attaggaatt
     2641 tcatcggtca gcattaacgc caccattagc accagacaga aaatcgcatg gggtgcctgg
     2701 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
     2761 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
     2821 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg ctttagcccc
     2881 actacattca atccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaataagt
     2941 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
     3001 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
     3061 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
     3121 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagca
     3181 ccataacgtt cacgtaattt gagatcgtcc agccgttgac caatcattgg cgatccgggg
     3241 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
     3301 gttcggcgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
     3361 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
     3421 aagaaactaa aaccatgata gccttcacgc agcaattcac tgttgactac caggttcggc
     3481 ggcgtcgcca ccagcgtcat catgccgctt atcagcccgg caaaacttaa cggcatcatc
     3541 aggcgcgacg gcgacgtttg catacgcatg gcaacgctta acaccacggg aataaagata
     3601 gcgacaacgc cggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
     3661 accaacattt tgatttcact gttgcccgcc actttgacca gccatgtccc cattacagtg
     3721 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
     3781 ttaggatcgg aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
     3841 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
     3901 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
     3961 taattgaggg ggataacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
     4021 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
     4081 gctccagact gtggaggact aaatcgactt cattcaggcg cggggtatct gccggggcat
     4141 taaccgcaat aacatggcaa cccgccgcca gacctgaaag cacgccagcg ggagcatctt
     4201 ccaccacaac acactcctgc ggcgcaagcc ccagcagctg cgcgcctaac agatacgcat
     4261 caggttctgg ttttccgcgc ttcacccgct cagcggttac aaacacctct ggcgcgggaa
     4321 gccccgctat tttatgacgc gctcgcgcta ccggcatgga gccagaagtc acaatagccc
     4381 acggaatacc tgctttattc aaatgattga gtaaggcgat tgcccccgga agcgcggtaa
     4441 tgccttcggt ttccgtggcc tcgatgtgct ccagacgcgt aaactcggcg gcaatgtctg
     4501 cctcggattt gcccgccata aaatggcgca gagaggtgat cgcctgttta ccgtgaatga
     4561 aagccagcac ctcttccggc gataacccat gacgtctggc ccagttgctc cacgcccgtt
     4621 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
     4681 gcacgcgggc ctcctcaggc gttaataatt tgattaattt cgttggcgct caaatggtac
     4741 tgacgcgggc aggcatgcca gacattaagc atgcgctgat atttttccca cattggcgtc
     4801 tgggcgttaa aaccgtgagt tccagcgtca aaatgggtat agcgcccttc cacattaacc
     4861 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
     4921 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca
     4981 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
     5041 tcgcgatcca gttcacgcat ctgtaatcca taaccacgct caatgattgt ttgcaggcga
     5101 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg gttagacaaa
     5161 ataagacgtt gcgcgttggt catttccatt attgactcct gtatcactct actacggtga
     5221 aaaaaagaag gctgagtatg ccttctttta tatgcgtaat caggggtcaa ttacaaatca
     5281 tcaaggaaag ttttatccag ttgtttgaag gcacgcttaa gcgtgtcagc taatgcctgg
     5341 taatcaggct tgccttcaac gggtgccaac acctgtccag actcctgcaa tttaccgcga
     5401 acttcataaa accagttgag gattgcagga ggtaatggcg ttacagaacg cttgcccagc
     5461 caccacaatc cctgcatggg taaacttaag gcgaacagcg cagtggcaac tgccggccca
     5521 agctgaccgc ccagggcaat ctgccagcag agagtaaata cggcgatcgg cggcataaaa
     5581 cggatcgcat aacgcgtcat cttgataacg cgattttcga caaagactgg ggcaaggcgt
     5641 ttttccagcg gccacgtctt tgagtaatgc tgtccccggc gaaacaagct aaaaaaatta
     5701 acagaacgat tatccggcgt tgacatgctt cacctcaact tcacatataa agattcaaaa
     5761 atttgtgcaa attcacaact cagcgggaca acgttcaaaa cattttgtct tccataccca
     5821 ctatcaggta tcctttagca gcctgaaggc ctaagtagta catattcatt gagtcgtcaa
     5881 attcatatac attatgccat tggctgaaaa ttacgcaaaa tggcatagac tcaagatatt
     5941 tcttccatca tgcaaaaaaa atttgcagtg catgatgtta atcataaatg tcggtgtcat
     6001 catgcgctac gctctatggc tccctgacgt ttttttagcc acgtatcaat tataggtact
     6061 tccatgtcga gtaagttagt actggttctg aactgcggta gttcttcact gaaatttgcc
     6121 atcatcgatg cagtaaatgg tgaagagtac ctttctggtt tagccgaatg tttccacctg
     6181 cccgaagcac gtatcaaatg gaaaatggac ggcaataaac aggaagcggc tttaggtgca
     6241 ggcgccgctc acagcgaagc gctcaacttt atcgttaata ctattctggc acaaaaacca
     6301 gaactgtctg cgcagctgac tgctatcggt caccgtatcg tacacggcgg cgaaaagtat
     6361 accagctccg tagtgatcga tgagtctgtt attcagggta tcaaagatgc agcttctttt
     6421 gcaccgctgc acaacccggc tcacctgatc ggtatcgaag aagctctgaa atctttccca
     6481 cagctgaaag acaaaaacgt tgctgtattt gacaccgcgt tccaccagac tatgccggaa
     6541 gagtcttacc tctacgccct gccgtacaac ctgtacaaag agcacggcat ccgtcgttac
     6601 ggcgcgcacg gcaccagcca cttctatgta acccaggaag cggcaaaaat gctgaacaaa
     6661 ccggtagaag aactgaacat catcacctgc cacctgggca acggtggttc cgtttctgct
     6721 atccgcaacg gtaaatgcgt tgacacctct atgggcctga ccccgctgga aggtctggtc
     6781 atgggtaccc gttctggtga tatcgatccg gcgatcatct tccacctgca cgacaccctg
     6841 ggcatgagcg ttgacgcaat caacaaactg ctgaccaaag agtctggcct gctgggtctg
     6901 accgaagtga ccagcgactg ccgctatgtt gaagacaact acgcgacgaa agaagacgcg
     6961 aagcgcgcaa tggacgttta ctgccaccgc ctggccaaat acatcggtgc ctacactgcg
     7021 ctgatggatg gtcgtctgga cgctgttgta ttcaccggtg gtatcggtga aaatgccgcg
     7081 atggttcgtg aactgtctct gggcaaactg ggcgtgctgg gctttgaagt tgatcatgaa
     7141 cgcaacctgg ctgcacgttt cggcaaatct ggtttcatca acaaagaagg tacccgtcct
     7201 gcggtggtta tcccaaccaa cgaagaactg gttatcgcgc aagacgcgag ccgcctgact
     7261 gcctgatttc acaccgccag ctcagctggc ggtgctgttt tgtaacccgc caaatcggcg
     7321 gtaacgaaag aggataaacc gtgtcccgta ttattatgct gatccctacc ggaaccagcg
     7381 tcggtctgac cagcgtcagc cttggcgtga tccgtgcaat ggaacgcaaa ggcgttcgtc
     7441 tgagcgtttt caaacctatc gctcagccgc gtaccggtgg cgatgcgccc gatcagacta
     7501 cgactatcgt gcgtgcgaac tcttccacca cgacggccgc tgaaccgctg aaaatgagct
     7561 acgttgaagg tctgctttcc agcaatcaga aagatgtgct gatggaagag atcatcgcga
     7621 actaccacgc taacaccaaa gacgctgaag tcgttctggt ggaaggtctg gtcccgacac
     7681 gtaagcacca gtttgcccag tctctgaact acgaaatcgc caaaacgctg aacgcagaaa
     7741 tcgtcttcgt tatgtctcag ggcactgata ctccggaaca gttgaaagag cgtatcgaac
     7801 tgactcgcaa cagcttcggc ggtgcaaaaa acaccaatat taccggcgtt atcgttaaca
     7861 aactgaacgc tccggttgat gagcagggtc gtacccgtcc ggatctgtcc gagatttttg
     7921 acgactccac caaagcaaaa gtgaacaacg ttgatccggc gaagctgcaa gaatccagcc
     7981 cgctgccagt tctcggcgct gtgccgtgga gctttgacct gatcgcgact cgtgcgatcg
     8041 atatggctcg ccacctgaat gcgaccatca tcaacgaagg cgacatcaat actcgccgcg
     8101 ttaaatccgt cactttctgc gcacgcagca ttccgcacat gctggagcac ttccgtgccg
     8161 gttctctgct ggtgacttcc gcagaccgcc ctgacgtgct ggttgccgct tgcctggctg
     8221 ccatgaacgg cgtagaaatc ggtgccctgc tgctgactgg cggctacgaa atggacgcgc
     8281 gcatttctaa actgtgcgaa cgtgctttcg ctactggcct gccggtattt atggtgaaca
     8341 ccaacacctg gcagacttct cttagcctgc agagcttcaa cctggaagtt ccggttgacg
     8401 atcacgagcg tatcgagaaa gttcaggaat acgttgctaa ctacatcaac gctgactgga
     8461 tcgattctct gactgccact tctgagcgca gccgtcgtct gtctccgcca gcgttccgtt
     8521 atcagctgac tgaacttgcg cgcaaagcgg gcaaacgtat cgttctgccg gaaggtgacg
     8581 aaccgcgtac cgttaaagca gccgctatct gtgctgaacg tggtatcgca acttgcgtac
     8641 tgctgggtaa tccagcagag atcaaccgtg ttgcagcctc tcagggtgta gaactgggtg
     8701 caggcattga aatcgttgat ccagaagtgg ttcgcgaaaa ctatgttggt cgtctggtcg
     8761 aactgcgtaa gaacaaaggc atgaccgaaa ccgttgcccg cgaacagctg gaagacaacg
     8821 tggttctcgg tacgctgatg ctggaacaag atgaagttga tggtctggtt tccggtgctg
     8881 ttcacaccac cgcaaacacc atccgtccgc cgctgcagct gatcaaaact gcaccgggca
     8941 gctccctggt atcttccgtg ttcttcatgc tgttgccgga acaggtttac gtttacggtg
     9001 actgtgcgat caacccggat ccgaccgcag aacagctggc agaaatcgcg attcagtccg
     9061 ctgattccgc tgcggccttc ggtatcgaac cgcgcgttgc tatgctctcc tactccaccg
     9121 gtacttctgg tgctggtagc gacgtagaaa aagttcgcga agcaactcgt ctggcgcagg
     9181 aaaaacgtcc tgatctgatg atcgacggtc cgctgcagta cgacgctgcg gtaatggctg
     9241 acgttgcgaa atccaaagca ccgaactctc cggttgcagg tcgcgctacc gtgttcatct
     9301 tcccggatct gaacaccggt aacaccacct acaaagcggt acagcgttct gctgacctga
     9361 tctctatcgg accgatgctg cagggtatgc gcaagccggt taacgacctg tcccgtggcg
     9421 cactggttga tgatatcgtc tacaccatcg cgctgactgc gattcagtct gcacagcagc
     9481 agtaatctcg tcatcatccg tagctttacg ctgcggatat ctgaaccgga aatagtcact
     9541 atttccggtt ttttattctc ttaatttgca ttaatccttt ctgattatct tgcttaactg
     9601 cgctgcatca atgaattgcg ccatctcact ttgcatactt accactttgt tttgtgcaag
     9661 ggaatatttg cgctatgtcc gcaatcactg aatctaaacc aacaagaaga tgggcaatgc
     9721 ccgatacgtt ggtgattatc ttttttgttg ccattttaac cagccttgcc acctgggtag
     9781 ttccggtggg catgtttgac agtcaggaag tgcagtatca ggttgatggt caaacaaaaa
     9841 cacgcaaagt cgtagatcca cactcatttc gcattctgac taacgaagca ggcgaacctg
     9901 agtatcaccg cgtacagctg ttcacgacgg gcgatgaacg cccgggcctg atgaacttcc
     9961 cgtttgaagg gttaacctca ggatcgaaat acgggacagc cgttggcatc atcatgttta
    10021 tgctggtgat tggcggcgcg tttggcattg tgatgcgtac aggaaccatt gataacggta
    10081 tcctggcgct tattcgccat acccgcggaa atgaaattct ctttattcct gcgctgttta
    10141 ttctgttttc acttggcggc gcaatatttg gtatgggaga agaggccgtc gcctttgcca
    10201 ttatcatcgc accgctaatg gtccggctgg gctatgacag tattaccacc gtccttgtga
    10261 cctatattgc cacgcaaatc ggttttgcca gttcgtggat gaacccattt tgtgtggttg
    10321 ttgctcaggg tattgccggc gttccggtgc tttctggctc cgggttgcgc atcgtggtgt
    10381 gggttatcgc cactctgatt ggcctgatct ttaccatggt gtacgcctca cgagtgaaaa
    10441 agaatcctct tctgtcacgc gtgcatgagt ccgaccgctt ctttcgtgaa aagcaggctg
    10501 atgttgaaca acgtccgttt acctttggtg actggctggt attgattgtc ctgaccgccg
    10561 taatggtctg ggttatttgg ggcgtgatcg ttaatgcctg gtttattcct gaaattgcca
    10621 gccagttctt caccatgggt ctggtgattg gcatcatcgg cgttgttttc cgccttaacg
    10681 gcatgacggt taataccatg gcttcatcct ttaccgaagg ggcgcgaatg atgatcgccc
    10741 ctgccctgct ggtgggtttc gccaaaggga ttttgctgct ggtcggtaat ggtgaagcgg
    10801 gtgatgccag cgtgttaaat accatcctca acagcattgc caatgccatt agcggtctgg
    10861 ataacgcagt cgcggcctgg tttatgttgc tcttccaggc ggtatttaat ttcttcgtga
    10921 cgtccggttc tggtcaggcg gcgttaacca tgccgttact ggcaccgctt ggcgatctgg
    10981 tcggtgttaa ccgtcaggtt accgtgctgg cgttccagtt tggtgatggc ttcagccaca
    11041 tcatttaccc aacctcagct tcgttaatgg cgacgctcgg tgtttgccgg gtggacttcc
    11101 gtaactggct gaaggtgggt gcgaccctgc ttggactgct gtttattatg tccagcgtcg
    11161 tggtgatcgg cgctcagttg atgggctacc actaaaaatg ttaagagccg cgattgcggc
    11221 tcttttttca ttctgccgtt tcagtctcga cggcttcatt tttggcattg cgcttcatcc
    11281 acaacgccag cgctttcagc gagtctggag tgaactcatc gcagcgtgcg gtgatttctt
    11341 ccggcgtcag ccagcaaact tcactgactt catcttcctg tagagcgaag ggaccgtgag
    11401 agacgcagct gaacaacgcg ccccagacac ggcaattttt atcttcgaaa tagaactgcc
    11461 cgtgctcggc aaaggggacc ccggcaatgc ccaactcttc ttccgcttcg cgacgcgcgg
    11521 attccagcag ttgctcatcg gcctggacta caccgcctgc ggtcgcatct aacatgccgg
    11581 gtaaaaagtc ttttgtctcg gtacgacgct ggaccagaat tttgcccatg ccatcatgca
    11641 cgacgatata agttgcacga tgacgcagac actgtgcccg catttgttcc cggctggctt
    11701 gtgcaatgac ttcgttctct tcattgacaa tatccaccca ttcagtactt gccaaacgac
    11761 gctgttccat tatcaggaaa ccttcttttt ctggcgctct tacggcgcat tttgagttgt
    11821 gggtaaatta cggattaatc gcgacctgcg caatgatact ttgatcattg agtgcgatta
    11881 cactaagtac gtcattatcc agcatgccat aactcgccgg attaccgcct ttcggaatac
    11941 tcaccgagcc ggggttgaag tggaaaatct ccccccgctg ttctgccact ggcagatggg
    12001 tatgaccgta caccagcaca tcgttctggt ttaaagcagg tagattttcc gggccaaaaa
    12061 gatgaccatg cgtcaaaaac agacgttgtt tttccagtaa tacctgttgc cacggcgcgg
    12121 ttatcgggaa atgcagcagc atttgatcca cttcgctgtc gcagttgccg cgcacagcga
    12181 taaccttatg tgccacttca ttaagccgtt cagcgacttt ggctggcgcg taaccctccg
    12241 gtaaagcatt acgcgggcca tgattcaaca cgtcgccaag gatcaccagc cactgggcac
    12301 cgctttggac aaacaactcc agaacacgtt ccgtcgccgg taacgaccca tgaatgtccg
    12361 atgcaaacat cagtttcatc actcactcct cgtcgaaaaa acgtaagcta tgatactgga
    12421 ttctgtcgtt gctatcagcc agattgcttc gccgaaagtg caataaaacg ctggacagac
    12481 gctcgctgcc actggaatgt cgcataatcc accaggcgtt ccggcacctc atcgccatgt
    12541 agtaccaggc ggttaatcat tagcgccaga tcagtatcag caatgcacca ttcaccaaat
    12601 aaattcggct gacccagtgc taacaaatgt tctgccatcg cgaacagttt ctctgcactg
    12661 gcttttccct cggccgttag tggcgctttt ttcgcccccg caaagacaac atccgtcggg
    12721 cgttcttcgc ggatgggcat cagatcgctg cgcaaccagg cctgaatctg tcgtgcacgc
    12781 gcacgatttt ctaaatcaag cggataaata cgttcccagg tcggtggcgc aaatcgatct
    12841 tccagatact ccgcaatggc agaagattca ctcaactcaa aatcatcgat ttgtaataac
    12901 ggcacacggc gtgtctgacc gtaaccttgc cacgccggct gcaaatgttc accgctgtcg
    12961 aggtcgatgg tcttgatatg aaacgacagg cctttctcct gcaacgccac ccaggcggat
    13021 aacacataag gggagaaaaa atgggcatct gaccaaagcg tgatagcggt tttactcatt
    13081 atgtcctcgg caataaaggg ggcttgctat caaactatag agcctttttg tcactgtcac
    13141 gtgacgaata ctcacgcaat tcgccgcgct ttctatactt gtggtgttca gatgattaga
    13201 caaccggagc tgcaatgatc gatctctatt tcgccccgac acccaatggt cacaaaatta
    13261 cgctgtttct cgaagaagca gggctggatt atcgcttaat aaaggtagac ctggggaaag
    13321 gaggtcagtt tcgcccggaa tttttgcgca tttcgcctaa caacaaaatt ccggcaattg
    13381 ttgatcattc tccagccgat ggcggcgaac cgctaagcct ctttgagtct ggtgccattt
    13441 tgttgtatct ggctgagaaa acgggactct tcttgagtca tgaaacgcgt gaacgcgccg
    13501 tcacattaca gtggttattc tggcaggtag gcggactggg gccgatgctt gggcaaaatc
    13561 atcattttaa tcacgcaacc ccccaaacca ttccttacgc tattgaacgt tatcaggttg
    13621 aaactcagcg tctgtaccat gtactgaaca agcggctgga aaactcgccc tggctgggtg
    13681 gcgagaacta cagcattgcg gatattgcct gctggccgtg ggttaatgcc tggactcgcc
    13741 agcgaattga cctcgcaatg tatccggcag tcaagaactg gcatgagcgg atccgttcgc
    13801 gccctgccac cgggcaggca ctgctaaaag cacaacacgg tgatgagcgt tcggatagtt
    13861 aacagaaaca ggttctcgtg tattatttca ttctaagtaa aacaacggag aacctgcaat
    13921 ggcacaacct gccgctatta ttcgtataaa gaaccttcgt ttgcgtacgt ttatcggaat
    13981 taaggaagaa gaaattaaca accgtcagga tattgttatc aatgtgacta tccactaccc
    14041 cgccgataaa gcgcgcacta gcgaagatat caacgatgcg ctgaattatc gcaccgtaac
    14101 gaaaaacatt attcagcatg tagagaataa ccgtttctct ttgctggaaa aattaactca
    14161 ggatgtgctc gatatcgcac gtgaacatca ctgggtgacg tatgctgaag tggagatcga
    14221 taaactgcac gcgctgcgct acgccgattc ggtatccatg accttaagct ggcagcgtta
    14281 accgccatat cgggaggctg catgaacata gtgatcaccg gagggacggg attaattggt
    14341 cgccatttga ttccacgttt gctggacctg ggccatcaaa tcacggtagt gacgcgtaac
    14401 ccgcagaaag ccagttccgt tctcggccct cgggtgacac tatggcaagg gcttgccgaa
    14461 caaagcaacc tcaacggcgt tgatgcggta atcaacctgg ccggagaacc gattgctgat
    14521 aaacgctgga ctcacgagca aaaagagcgt ctctgccaaa gccgctggaa tatcacgcaa
    14581 aaactggtcg atttgattaa tgccagcgac acgccgccgt cggtactcat ttccggctcg
    14641 gcaacgggct attatggcga cttaggtgaa gtggtggtta ccgaagagga accgccgcat
    14701 aacgaattta cccataaact ctgcgcccgc tgggaagaga ttgcctgtcg ggcgcaaaat
    14761 gacaaaacgc gagtgtgcct gctgcgtacc ggcgtagtgc tggcaccaga cggtggtatc
    14821 ctcggtaaaa tgctgccgcc atttcgtttt ggcctgggcg ggccgattgg ctctggtcgg
    14881 cagtatctgg cctggattca tattgatgat atggtcaacg gtattctctg gctgctggat
    14941 aacgagctgc gcgggccatt taatatggtt tcgccctacc cggtgcgcaa tgaacaattt
    15001 gcccatgcac tgggtcatgc gctgcatcgc ccggccattt tgcgcgtccc tgcgaccgcc
    15061 attcggttgt taatgggcga atcttcagtt ctggtattag gcggtcaacg cgcgctgcca
    15121 aaaaagctgg aagaagcggg ttttgcgttt cgctggtacg atttagaaga ggcgctggcg
    15181 gatgtcgttc gctgatgtgg tttactgcaa acatccgcca gcggaaacgc tgatgaataa
    15241 atatagataa ttcagaacta tttttagcgg actgcaactt ttacatttca gcggaagcgg
    15301 actcgcaaat tccccatgaa atactccggc agcgttaacc accggagagg aaattatttc
    15361 agcgaacctt taaggaactg ttgcagacga gggctttgcg ggttaccgaa taactgctcc
    15421 ggcgcccctt cttcttctat tttcccctga tggaggaaaa tgacgtgagt agaaacatga
    15481 cgagcaaagc ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc
    15541 tgcataatac gcaacacttc gcctaccagt tcaggatcga gcgctgaagt aggttcatca
    15601 aacagtaaaa cttccggttc catcgctagc gcccgcgcga tagaaacacg ctgttgctga
    15661 ccaccggaca ggtgtaccgg gtatttcccc tgcgcacgtt cgtctatccc gacttttgcc
    15721 agatatttca ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc
    15781 gcttccatga cgttttccag caccgtcata tggctccaga gattgaagtg ctggaatacc
    15841 atcgtcaggc gtgtgcgcaa taagcgcagc tgatttttat cggcgacttt gagttgaccg
    15901 tctttatcgc gcaccagatt gatcgtctgg ccattgacca cgatcgatcc ttcgctcggt
    15961 ttttcgagga agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg
    16021 atgcttatga catctccggc attcgcttgc aatgagaccc ctttcagcac ttcgtgttcg
    16081 ccgtagcgtt tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg
    16141 cgttgaagaa ggtttcacat gctgcaacca gcgtttttca gctctacgaa agaggctgat
    16201 caggacataa gagatgatta aatagagcac cgcggcaatg ccaaaagcgg taaaaggttg
    16261 atatgtggcg gcgttaatat cgcgggcaat tttcagcaga tccggcaccg tggcagtaaa
    16321 tgccagcgca gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat
    16381 acgcagcgca gaaggcaaaa taatgcagcg atacatttta aaggtcgaga agccataggc
    16441 tctggcggct tcaatttccc catgcggcac tgaacggatt gccccggcaa aaatctcggt
    16501 agtgtaagcg caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcgaaa
    16561 aaaggcgtta aggaactcag ttcccttaac aatctccagc gtgtacatgc cggaatagaa
    16621 caccagcaac tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg
    16681 aaactggatg tatttattac ttgagacgcg accaatcgcc agaaacagcg ccagaactcc
    16741 gcctatcact accgacaaaa tcagcagcca cagggtgatt gctacgccag taaagcgata
    16801 accgtcagtc cacagcagtg gtttccagta ttcatgtaag atttcgatca caggtcagcc
    16861 ctcttcacac ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacggtg
    16921 gtgaaaacaa gataaatcac gccgcagacg atagcgaaat agaacggttc ccaggtactt
    16981 ttgcctgcca gctgcgtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg
    17041 gtagatttga ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc
    17101 gccggaaaca tgatccgccg aaacacttgc ccacgagtaa aaccgaacgc cgtcgccgcc
    17161 tctatatgtc ctttcggcac tgccataaac gctccacgaa acgtttcggt aaaataagca
    17221 ccgtagataa aaccgagagt gataatacct gcgaccatcg gatcaatatc aatctgcccg
    17281 acgcccatcg cctccgtcac cgtgtttagc gcaatctgca aaccgtagaa aatcagcagc
    17341 atcaatacta aatccggcac gcctcgaatc agcgtggtgt acccttcgaa aataaggccc
    17401 gaaagccgat tttgtgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc
    17461 actacagagc tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa
    17521 aacccataca acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc
    17581 tccggatacc cgaagggagc gggctttctt tcaccgatgg cgattaacca ccataaacat
    17641 caaaatcgaa gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa
    17701 aggctttgtt cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc
    17761 ctacgccaaa cagtttttca tctttaacag acgggccacc gaatttgtaa tctttaccga
    17821 cgggttgttt gaggaaacct tcgctggctg cgacctcatc ctggaacgcg gcatcaatac
    17881 gtccggcagt caggtcagaa taaatattgt cctgcccctg ataagaaacg atttcaatgc
    17941 cttttggtgc ccagtgttcg ttaccgaacg tctcctgggt ggtgccctgc aatacgccta
    18001 cccgtttgcc tttcagcgat tcgactgtcg gctgaatgtc ggaatttttc gccaccacca
    18061 gacgagaatc ggcagcgtac agtttgtcgg tgaaggctat ttcttgctga cgtttttccg
    18121 taatggaaag cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg
    18181 catccagtgg gttttcgaca aacgtacatt gcgtattgat gcgtttgcac aactccttcg
    18241 ccagatcgat atcgaagcca accagttcac cttgtgaatt ttttgattca aatggcgcat
    18301 aggtcggatc ggtaccgatg cggatgtttt gcggaatcgc agcaaatgcc gcagttgcgc
    18361 tggagaaggc cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg
    18421 acagtctttt tatcgggaat atttacaggt ttgttacact tatcgtgcca taaaattgac
    18481 catcaaggca aaatatcccg ccccgctaga cgtattattt ttcattaaaa atgcttaagt
    18541 atgcatttca atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc
    18601 atcaggtaca gcttccctgc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt
    18661 ttcgccatct tgtcgtaggt accgtcctga cgcagctcgc caagcgcctt attaaacgca
    18721 gccgtcagtt cagcatcatc tttacgcagc cctacaccgg tgccgtcacc gaagtatttt
    18781 ttgtctttca ctgatgggcc tgcaaaggcg aaatctttac cggcgggttg cttaaggaaa
    18841 ccttcgctgg cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg
    18901 gaatagacta aatcctggtt ggcataggcc accacatcca cgcctttgct acgccaggtc
    18961 tcgttagcgt atgcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt
    19021 gaatccagcg ttggctgaat cggtgaacct ttggcggcaa tcaaacgaga atctgcggcg
    19081 tacaacttgt cggagaaagc aatctcctgc tgacgtttat cggtaatgga gagcgacgaa
    19141 ataatggcgt cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcgctggca
    19201 acccaggtac atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag
    19261 ccaacaaaat cacctttagc atctttcgac gagaacggcg cataggtggt atcggttccg
    19321 atacgtaccg tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaac
    19381 aaagacagag cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta
    19441 tgttgtgtat tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac
    19501 gacagcagta acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt
    19561 tgaagatagc aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat
    19621 gtcgaggatt tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc
    19681 gccctgccag cgggcaaaga gatcttcagg aagggttacc gcaaactggt caagaacacg
    19741 attaaccgtc tgatttatca catcatcaag ggattgtggg cgatgataaa acgccggaac
    19801 gggaggcata atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa
    19861 gtgcagtggc gtttcacgca cgcagagcac caacgggcga cgttctttca gcaccacatc
    19921 tgccgcacgg gtcagtaagc catcagtata gctatggaca atgccggaca gggttttgat
    19981 tgaacagggt aaaatcacca tccccaacgt ctggaaagaa ccggaagaaa tgctggcagc
    20041 aatatcgcgc gcatcgtgtg tcacatcagc taacgcctgc acttcgcgca gggaaaaatc
    20101 cgtttcgagg gataaggtct ggcgcgccgc ctggctcatt accagatgcg tttcgatatc
    20161 tgtgacatcg cgcagaacct gtaataaacg cacgccataa atcgcgccgc tggcaccgct
    20221 gatgcctaca atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg
    20281 caggatttcg gcgggagttg caagtcaggg tgccagaccg gcaccctgag tgaaggcatc
    20341 atccttcgtt atgcatttcg agattttcca cttcgttctg acgttgcact gctttggcgt
    20401 cgtcattacg taaagtatca aggaaatcga ggtagccctg atcaacgtct ttggtgacgt
    20461 agacgccgtt gaacaccgag cattcaaact gctggatatc cgggttttca gcgcgaacgg
    20521 cttcgatcag atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg atctggcgaa
    20581 tttcatctac ttcgcgaccg tgagcgatca gttccgtggc gctcggcata tcgataccgt
    20641 aaacgttcgg gaagcgaatt tccggtgccg cagaagcgag gtacactttc ttcgctccgg
    20701 cttcgcgtgc catctcgata atctgctcag aagtggtgcc acggacgata gagtcgtcga
    20761 ccagcaggac gtttttatcg cggaactcgg cgcggttggc gttcagttta cggcgcacgg
    20821 acttacgacg cagctgctga cccggcatga tgaaggtgcg gccaacataa cggtttttaa
    20881 cgaagccctg gcggtacggt ttgcccagaa tacgcgcaat ttccagcgcg atatcacacg
    20941 aggtttccgg aatagggatc accacgtcga tatccagatc ttcccattcg cgggcaattt
    21001 tctcgcccag tttggtgccc atattcacgc gcgcgctgta aacggaaatt ttgtcgatga
    21061 acgagtccgg gcgggcaaag tatacatact caaacaggca cggattgctg accggattgt
    21121 cagcacattg acgggtaaat aactgccctt cttcagtgat gtaaatcgct tcgcctggcg
    21181 cgacgtcacg caggaaatca aagcccagcg tatcgagcgc aacgctttcg gaagcgacca
    21241 tatattctgt gcggttctcg tcaatatcac gttttcccag taccagcgga cgaatcccgt
    21301 taggatcgcg gaaagcaacc ataccgtggc cgatgatcat cgccacacag gcatacgcgc
    21361 cgcggattaa gcggtttgta gcagcgatgg cggcgaaaat gttgtcggct tccagcgggt
    21421 agtggcggaa gttgtccagc tcgctggcga agatattaag cagaatttcc gagtcggaag
    21481 tggtgttgat gtggcggcgt ttttcttcaa acagtttttt acgcaactcg tgagcgttgg
    21541 tcagattgcc gttgtgggca agcgtaatgc catacgggga gttaacgtaa aacggctgcg
    21601 cttcagaggc gctggagctg ccagccgtgg ggtaacgcac atgaccaatg cccatattgc
    21661 cctgcaaacg ctgcatatgg cgagcttcaa atacatcgct caccagcccg ttcgctttac
    21721 gcaaacggaa gcagttattg gcatctatgg tgatgatgcc ggcggcatcc tgaccgcgat
    21781 gctgaagcac cgttaaggca tcataaatcg actggttaac cggcataaca ccggcgatac
    21841 cgacaatacc gcacatacgt ctttttcctc gttaagccac atctcagagc acttacgctc
    21901 tgggcaagaa acttgacgag ctttgcagat aatcaaaaaa ccatctgatg ataaaactga
    21961 actgcgggat cagctgtgat ttgctccagt cttcgctttt cgacaccccg gtaaaggagt
    22021 cgagaaagaa gagaatggca gcaacaatca acacaccgcg caacgcaccg aaacagacgc
    22081 ccaacacccg atcggtgcct gacaaccccg ttttctccac caactggcct atcacgaagt
    22141 tcacgatagc accaacgatc agggtagcga taaacagtac cgcgatggca atcccatttc
    22201 gaaccagttc gtcttcaaag cccgtaaacc agactgacag gtaagtgtag taatgactgg
    22261 caacaaagaa agcacaaccc catgtcacca gcgataacgc ttcacgaaca aagccgcgga
    22321 tcaggctaac cagagaggaa aaagcaatca ccgcgattat ggcgtaatca atccagacca
    22381 tatgtgtccc acgattttac gccctgtcat cctgttcggg gcgcattcta acagaaaaag
    22441 aaaacgtttg cgtagggatt tccttcccgc gcatcaataa aaatggcgct gaaaaaatat
    22501 tcaacgccat cgacttttta tgcctttgcg gcatcgggca atgcgtgtcg gatgccgcat
    22561 gaacgcctta tccgacctac ggttctaccc ctgcgtaggc ctgataagac gcgctggcgt
    22621 cgcatcaggc aacggctgtc ggatgcggcg tgaacgcctt atccgaccta cggttctacc
    22681 cctgcacagg cctgataaga tgcgccagcg tcgcatcagg caagaccgta ttaattcggt
    22741 gtatagccca ttaccacgcc acttaagcca gaaagctgct tgagctcacc cagcgatcct
    22801 ttcagcttct ctttggaagc atccggccca accagaatac gggtaatttt accctgcact
    22861 ggcgtggatg gcgacgtata aacccgataa ccggcaccgc gcagcttacc gacaatctca
    22921 ttcactttat cggcattttt cagcgcgccc agttgcacaa cataagcttt acccgttggc
    22981 gctgcttttt cttccacaac cggcttcggt tctggcttcg gcgcaggtgg tgcttcaacc
    23041 tttggcttaa gcggctccac cagtttcggc ttcggtgggg cgaccggtgc aggttccggt
    23101 tcaaactcgg tgttattggc tgcaatagtg gccggatcga gcgacggtgc tgcggcatca
    23161 cctgcccgta cctcttccgc tgcgccttcc ggcggctgcg tcggcaacgc ctgggtggcg
    23221 gctggcatca tatcaggctc atcgcgatcg cccgcttttg gcaccagcgg gatagccgcg
    23281 aactcatcct gataatgttt tttctgcccg tccagcagcc ctggaagtac aatcaccccc
    23341 agcgctacca gcacgatcgt gcccactaac cgattctgaa acttacttgc caccgcttct
    23401 cctcgcgtca atcacttcca tgacatgtgc gaccgtgtgg aacgaaccac acaccagcac
    23461 ggtgtcttcc gctttagcgt ccgccattgc ggcatcccat gcctgcgcaa cgctatcaaa
    23521 tgatttgccg ttacccaaat gctcaagcag ttgttctgcc gtggcaccgc gcggcccttc
    23581 cagtggcgca caataccagt catcaaccac gcttttcaac caggccagag ttccggcaat
    23641 atctttatca tgtagcatac cgataaccgc cagcacgcgc ccgtttttcg gtagcgcttt
    23701 catacgcccg gtgagatatt ccgccgcatg tggattatgc gcgacatcaa aaataacgcg
    23761 tggcgactcg ctcacaatct ggaaacgtcc cggcaaaatt gcgctggcaa tcccgtcgcg
    23821 aatggcattt tcactgacct ccagcccgct ggcacgcagt gccgccagcg ccgttgcggc
    23881 attcggttgc gggacaagcg gcaacggcag attttccagc gtgccgtgag catcgctaaa
    23941 cgtccagtca tgatcggtga cggaatagtt ccactcaacg ccccgacgtt gtagcagtgc
    24001 acctttttcc tgcgccacat cagcaatggt agaaggcatc tccggctcac cgacaattgc
    24061 cggtttttcg ctgcgaaaga tacctgcttt ctcgcgacca atactttcac gatctggccc
    24121 cagccagtcg gtatgatcca gcgcaatact ggttactacc gcgacatcgg catcgacaat
    24181 attggttgcg tccagacgac cgcccagccc tacttccaga atcactacgt caagttgtgc
    24241 ctgcttgaac agccacaacg ccgagagcgt accgtactcg aaataggtca gggaaatatc
    24301 accgcgtgcc gattcaatct ccgcaaaaga agcggtgtgg gccgattccg gcaattcctg
    24361 accctgcaca cgtacgcgct cggtataacg caccagatgc ggcgaactgt agacgcccac
    24421 tttgtaccct gccgccatca gaatcgactc cagcgtacgg caggtggtgc ctttgccatt
    24481 cgtacccgca acggtaaaca caaatggcgc tggtttcaga acgccaagac gcgccgcgac
    24541 ctggctcacg cgctcaaggc cgagatcgat agttttactg tgcaggtttt ccagataaga
    24601 aagccacgaa gccagaggcg acgcggcttg aggagtgcgt ttgataatca tggtatccgc
    24661 tgattcgtta cggtgagaat agcaaaaggg cagagccagt ggccctgccc ttatcagtta
    24721 tcaggcctca ggttcctgat ccggtaccgg tggcaccact acgccttcac gcggcgcttc
    24781 aggattcggc gctggcagat tcatcaactt cgccagaatg ctcgccagtt tcaggcgcat
    24841 ttccggacga cggacgatca tgtcgatcgc gcctttctcg atcaggaatt cactgcgctg
    24901 gaatcctggc ggcagttttt cgcgaacggt ctgttcgata acacgcggac cggcaaagcc
    24961 aattaacgct ttcggttcag cgatgttgag atcgcccagc atggcgaaac ttgcagaaac
    25021 accgcccatg gtcgggtcgg tcagcacgga gatgtacggc aagccgcgct cctgcatttt
    25081 tgccagtgcc gcagaggttt tcgccatctg catcagcgac atcagtgctt cctgcatacg
    25141 tgcgccacca gaggcggaga agcagatcag tgggcagtta tcttccagcg cctgctcaac
    25201 ggcacgcacg aaacgcgcac ctaccacaga ccccatggaa ccgcccataa aggcgaactc
    25261 gaatgccgca gcgacaaccg gcattccata cagagtgcct ttcatcacta ccagcgcatc
    25321 tttttcgccg gtttctttct gcgcagatgc cagacggtct ttatacttct tggagtcacg
    25381 aaacttcagc acatctttcg gctcaagctc gctacccagc tccacaaggc ttccttcatc
    25441 taacaggcta tgcaggcgat tacgcgctgt catacgcatg tgatggtcac acttcggaca
    25501 gacctcaaga ttacgttcca gctcagcgcg gtataaaacc tgaccgcagc tatcacactt
    25561 agtccacacc ccttcaggaa tgctcgcctt gcgggtggga gtaatgttgc ttttaattcg
    25621 ttcaatccag ctcattaggg acctttctgt ctgaacctgg ttcgatgcca gttttatctt
    25681 tggggacgca taatgccatt tttgccccca acagaccatg aatgttgcac attaaaacat
    25741 aacagcccga aactttggat aaaaaagtgg tcgaaccgca gagttacttt ttattttgcg
    25801 gcgcgtgccg ctgcgcgttt gtggcgaata aattcgaaaa cacctggcaa tattgaaaca
    25861 aaaataatgc caacaatcat cagtttaagg ttgctctgaa tgaaggggag cgtgccgaag
    25921 aaatagcctg cgtaggtaaa aagcagtacc cacaacagtg cgccaatcac gttataagcg
    25981 gcgaaatgac ggtacgacat gtggcccatt cccgcaacaa acggggcgaa cgttctgacg
    26041 atcggcacaa aacgggcgag aataatcgtt ttgccgccat gtttctcata aaactgatgg
    26101 gttttgtcga gataactgcg acggaaaatt ttcgaattgg gattactgaa taacttttca
    26161 ccgaacaacc gcccgatagt gtaattgacc gcgtcaccca caatcgcggc gatcagcatc
    26221 agcaccacca tcatatggac gttgagatcg ttggtctcca gtgatgccaa cgctccggca
    26281 acaaacagca atgaatcacc cggtaaaaac ggcgttacca ccagaccggt ttcacagaac
    26341 aaaattaaga acaaaatggc ataaacccag acgccgtact ccgcgaccag ctctgccaga
    26401 tgcacgtcaa tgtgcaggat aaaatcgatg aggaaataaa tcaggtccat atttgcctat
    26461 gccttgtact cgtcatctat caggctgtaa ctacgttggc tacagcctga aatgttccga
    26521 gcattattct cgttagtccg ccagaaatag cgggcccatt ggcggttttg gaagatcata
    26581 tcggtcaggg taatccaccg cgaccagata tagcccttcc gcttttgccg ttgctgccgc
    26641 cagcgttctg tcctttgccg ccagcaactc tgctatccag ctctccggct ggttgtgggc
    26701 accgacttcc atcaggctgc cgacaatatt cctgaccata tgatgtacaa aggcattcgc
    26761 tttgatatct accaccacat aaggaccgtg acgcgtgaca ttaatatgca taacgttgcg
    26821 ccacggggtc cgggactgac actgcaccgc acggaacgag gtgaaatcat tctcgcccag
    26881 caaacattgc gcagcccgat gcatccgttc agcgtccagc ggttcgtaaa aatgggttac
    26941 ccctttactc aataccgccg ggcgcagccg atgattgtag atgatgtagc gataacggcg
    27001 agccgtggcg ctaaatcggg catgaaaatc atcaggtaca gctttaaccc aacgcacggc
    27061 gatgtcacca ggtaaattcg catttacgcc taacgtccac gcagcgtctt tgcgctgcgc
    27121 ggtcgtttcg aaatgcacca cctgcccggt accgtgtaca ccggcgtcag tacgcccggc
    27181 gcagaagacg gtgatgggtt cgttcgccac ctgggagagc gccttttcca gcttctcctg
    27241 cacactgcgt acttcattct gccgttgcca gccgtaatac ctactgccgt cgtactcaat
    27301 gcccagcgca attttataaa ctggcggttg ttgctggtcg gacattagta cagatactcc
    27361 tgcaccagtt tctcggcgat ttttactgcc atcagcgcgc cgccaaagcg aacgttatcg
    27421 gccaccgacc agaactggac ttgctccggc ataccgtagt cattacgtac gcagccaaca
    27481 gaaagatgcg gcgtaccaga agcatcccct acctgagtcg ggaattcgtt ctcttcagag
    27541 agcacaatat cttcgccctg agcaaacgca tcacgcgctt cttctgccgc cagcggacgc
    27601 agtgcttcaa agttgaccat ctgggcatga ccgtagaata ccggtgcctg gacgacgctt
    27661 gccgaaatca tcagcccttc gtcctgcagg attttgcgta cttcgtcaac gatacgacgt
    27721 tcttcacgca cgctaccttc gctatccggc agtaacggca gcatgttgaa cgccagctga
    27781 cgcccgaaga aatcttcttc gtcaatcgga atgccattga gtaatttagc actctgcccc
    27841 gctaacgcat cgaccgcttt tttaccctgg gcggaggctg aaatcaggct ggtaacgctg
    27901 atacgtgata aaccgccctg atcaattagc ggtttcagtg ccgccagcag ctggctggtc
    27961 agactgtctg gtacggcgat gacattccgg ttgcggtaat cggtcagtac aaacgggttt
    28021 acttccggca ccaccagcgg tacgtcgggt tcgagagcaa acaatccgct gctgtcgatc
    28081 accagacaac ctgagttggt tgcttcttca acccaggtag cggtagcttc tttgcctgcg
    28141 acaaaaaacg ccagctgcgc ctgcgtccag tcgaattcag cggcatcctg cacggtgatt
    28201 gtcttaccac caaagcgcag ttgttctcct gcgctttcgt tacgtgccag tgcataaatt
    28261 tccccaaccg ggaactgacg ttcagccagc gtttcaagca gggcttcgcc cacagcgcca
    28321 gttgcgccca ggacggcaat gttccagcct tcagacatgg tggtttactc cagaaatagc
    28381 aaagctccct gccaatgtta cagcagagag catgaagaag agattaacgt gccggatgat
    28441 gaacggcgtt aaaacccagt ttacacagca atgatgccgc actggcgtca tcacaaatca
    28501 catacagaga tgaccattca cggcgctcaa gatagttttt gcgcagttta tcgaactcac
    28561 ccggtatccc ggcgacttta cgcagcggtg cgtcatcgcg gcgcacatca tacaccaaat
    28621 gcaccagcct tttcagcgtc ggttgatcga gcgggccatg cagcgtaatg cgaccaaact
    28681 ctggcgcggg cagtaatgta tccagcgcaa cgtgctgttc atgcccaata aacttgctat
    28741 aagcttcaaa cacttgcgta gtaccgcgtg ctttaccttc cagggtatag cctgcgatat
    28801 gcggcgtgcc gatatccact tttttcagca attccacgtt aagttccggt tcgccttccc
    28861 agacatccag cactacgctt aacttctggc cttcgctcag gcaggtcagc agggcggtat
    28921 tatcgacgac tgcgccacgg caggcgttaa tcaggatcgc tcccggtttc aggctgcgga
    28981 tcagtttttc atccgccaga tgcagcgttt tgtacggacc atctttaaag agtggcgtat
    29041 ggaaagtcag aatatccgca tgctggacta actcatccag cgagcggaaa tcaccctcat
    29101 ccccacggtc ggcgcggggc ggatcgcaaa gtaaggtttt gatccctaac gcttccagtc
    29161 gcgcctgtaa acgacgacca acgttaccaa cgccaacgat ccccacggta cgctcgtgca
    29221 gtgaaaatcc atcgcgttcg gcaagcatca gcagggagga gaaaacatat tccactaccg
    29281 caatcgcatt acagccaggt gcagcggaaa aaccaattcc cgcctgcttt aaccaggctt
    29341 catcgacatg gtcggtcccc gcggtggcag tgccaacaaa tttaatgggt tttcctgcca
    29401 gcaaagattc attcactttc gtgaccgaac gcaccataag cgcatccgcg tctgacagtt
    29461 gagcgacggg aattggacgc ccgggaaccg cggtcacctc acccaaacgg ctaaataagt
    29521 cgcgggcata aggcatattt tcatcaacaa ggattttcac gtttgtgtta cctgtatgag
    29581 acgagagtta accagacaag tgtgccataa tctcgcggcc aggcatactt gcgaagattt
    29641 caggtataag gatacgtaat gatacaacct atttccggcc ctcctcctgg gcaaccacca
    29701 ggtcagggag ataacctgcc gtctggcgcg ggcaatcagc ctttatccag tcagcaacgt
    29761 acttcgctgg aaagcttaat gacgaaagtg acctcactga cacaacaaca aagagcagaa
    29821 ctgtgggcgg gtatcaggca cgatattggt ctgtcgggag attcaccgct gctttcgcat
    29881 cacttccctg ccgctgagca taatctggcg caacgtctgt tggccgcgca aaaaagccat
    29941 tctgcccgcc agcttttagc gcaattaggg gagtatttac gtctggggaa taatcgtcag
    30001 gcggtcacgg attatatccg tcataacttt ggtcagacgc cgctgaatca gctctcaccg
    30061 gagcaattaa aaaccattct caccctgttg caggaaggga agatggttat tccgcaacca
    30121 cagcagcgcg aggcgaccga ccgtccttta ttaccggcgg agcacaatgc gctcaaacag
    30181 ctggtgacca aacttgcggc agcaacgggg gaacccagca agcagatctg gcaatcgatg
    30241 ctggaacttt ccggggtgaa agatggcgag ttaattccag cgaaactgtt taaccatctg
    30301 gtgacctggc tacaggcgcg tcagacgcta agccagcaaa atacgccgac actggaatca
    30361 ctacagatgg cgctaaaaca acctttagat gccagtgaac tggcggcgtt atcgacatat
    30421 atccaacaaa aatatggcct ttctgcgcaa tcatcgcttt cttctgccca ggccgaggat
    30481 attcttaatc agctttatca gcggcgggtt aaagggattg atccgcgtga tatgcaaccg
    30541 ctgcttaatc cttttccacc gatgatggac acgttgcaaa atatggcaac gcgtcccgcg
    30601 ctgtggatac tgttagtcgc gattatcctg atgctggtct ggctggttcg ttaaccccga
    30661 cgaaacgaca gtatcgtgac aataattccc agcaccgcag agatcgcccc ggcaagaaat
    30721 accgaagagt aaccaaacgt ggtcgccagc attcccgcaa gcggcccgga gacgccgagg
    30781 gcgatatcct gaaacgcggc gtaaccgccc agtgcggtgc cgcgaacttg tgaggggacg
    30841 cgtttaacca cctccacgcc cagcgcagga aagataagcg aacatccggc tccggttaac
    30901 gccgcgcccg ctaatgcgac ccacgcccct ggtgcttgcc agagcagcaa caagcccacc
    30961 gtttctacaa gcagagagac gatcgccact ttcacgccgc caaaacggtc cggcatccag
    31021 ccaaacatga cgcgcatcac gacaaatgcg ccgccaaacg cggtaagagt aaagcccgcc
    31081 atcgcccatc ctttgctggc aaagtagagc gaaacgaaag tcccgataac cgcaaaacca
    31141 acgccttgta gtgccagacc taaccctggt ttccagataa gcccgacaac gctccacagc
    31201 gatggacgtt ctcccgccag ggccggtact ttgcgcactg tgccgttaca ggcccacgcc
    31261 agtaagggta atgccattgt ggtgagcgcc agtgcggcaa aaccgtaatg gctatgaatc
    31321 aacaggccaa gcggcgcacc aacagcgagg gcaccgtaaa tcgccattcc gttccatgac
    31381 atcactttgc cggaatgttt tggcccaacg atgcctaatc cccaggtcag agcgcccgtc
    31441 agtaactggc tttcaccaaa gccaagaatc aaacgcccga cgaccaacag ggcaaatttg
    31501 aacggtgcgg agacgggcaa aatcgccgcc agcagcagcg cgccgcccgc cagaccacaa
    31561 gctaacatcc cctgaagcgc cgaacgtttt gcgccatatt gatcggccag tcgcccggcg
    31621 taaccacgcg tcagcaccgt agccagaaac tgaatgccga cggcaatgcc gaccatggta
    31681 ttgccatagc ccaattcatg atgaacaaac agcgggataa ccggcaacgg taaacctacg
    31741 gtcatgtagg tgagaaaaac cgcaaaagcg atgcggaaga gcgaaaaatt ggcagaagat
    31801 cgtgtttcgg tttggcttac agcagtcatg cattactcca gaatgcagcg caaggcgagg
    31861 agtatccccg tctcatctct ctggtttcag ggttacggtg cgttggcagg atttaacgca
    31921 tacgtctttt tagcaggata tcgacaaagc gggaagtttg cctggaactg gcggcgattg
    31981 tcaatgatgt gaaaaaggga accatcgggt tcccttttgc gttagtgccg gaggccgcat
    32041 tggtgcgtaa ccactccgaa ttaatctttc agcttgcgca ttaccagcgt ggcgttagtg
    32101 ccgccgaagc cgaagctgtt agacataacg gtggtcagtt cgcgatcggt cgtttcggtc
    32161 acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct cggggcgata
    32221 aaaccgtgtt ccagcatcag cagagagtag atagcttcct gtacgccagc agcgcccagt
    32281 gagtgaccgg tcattgcttt ggttgcagaa atcgccgggc tcttatcgcc gaacacttca
    32341 cggatagctg ccagttcttt cacgtcgcca accggagtcg aagtaccatg agagttcagg
    32401 tagtcgattg gggtatcaac accgtgcatc gccatcttca tgcagcgcac tgcaccttcg
    32461 ccagacggag caaccatgtc tgcgccgtca gaggttgcgc cgtagccaac gatttcagca
    32521 tagatgtgag caccacgcgc cagcgcgtgt tccagctctt caaccactac cataccgcca
    32581 ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc tttttccggg
    32641 gtgtcgttgt atttagtaga cagcgcaccc atcgcgtcga attcgcaagc catttcccag
    32701 cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg gatctgctct
    32761 actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga gtagttaacg
    32821 ccgtgaattt taaacggcgt ggcgaggcag gcagaaacgc cggatgccat cgctttggtg
    32881 accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc agcgccgaac
    32941 acctgaaaac gcggggaacc gccgccggaa cctgcaatca ggccaacgcg cgggttattc
    33001 tggtaagctt ccggggagag gccagcatct gcaattgcct gctccataga aaggaatgca
    33061 taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc agtggtatcc
    33121 agttttacgt tgccccagac gtggctacgc atgccggaat ccttcagctc ctgagagaaa
    33181 gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt attaccgatg
    33241 ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct ctgtaagtcg
    33301 cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac aagtccgatc
    33361 agccaattaa tagagaaatt tgcgcagcct gacacacatc gctaagatcg agccaccgcc
    33421 tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc tcgaatttaa
    33481 tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca acgataacgg
    33541 actggaagag acgcgttatg tttttcttgg gggtaaccat ttagaggcac gttttcctga
    33601 gcatccacat cctctgtttg tggtagcaga gagcggcttc ggcaccggat taaacttcct
    33661 gacgctatgg caggcatttg atcagtttcg cgaagcacat ccgcaagcgc aattacaacg
    33721 cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag cgctggcgca
    33781 tcaacactgg ccggagctgg ccccgtgggc ggaacagctt caggcgcaat ggcctttgcc
    33841 cttgcccggt tgccatcgtt tattgctcga tgaaggccgc gtgacgctgg atttatggtt
    33901 tggcgatatt aatgaactga ccagccaact ggacgattcg ctaaatcaaa aagtagatgc
    33961 ctggtttctg gacggctttg cgccagcgaa aaacccagat atgtggacgc aaaatctgtt
    34021 taacgccatg gcccgactgg cgcgtccggg cagcacgctg gcgaccttta cgtctgccgg
    34081 ttttgtccgc cgcggtttgc aggaagccgg attcactatg caaaaacgta agggctttgg
    34141 gcgcaaacgg gaaatgcttt gtggggtgat ggaacagaca ttacccctcc cctgctccac
    34201 gccgtggttt aaccgtacgg gcagcaacaa acaggaagcg gcaattattg gaggcggtat
    34261 tgccagcgcg ttgttgtcac tggcgctatt gcgtcgcggc tggcaggtaa cgctttattg
    34321 cgcggatgag gctcccgcac tgggtgcttc cggcaatcgc cagggggcgc tgtatccgct
    34381 tttaagtaaa cacgatgaag cgctaaaccg ttttttctct aatgcgttta cttttgctcg
    34441 tcggttttac gatttattgc ccgttaaatt tgatcatgac tggtgcggcg tcacgcagtt
    34501 aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcgatgg atttacccgc
    34561 agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg ttgcgacaaa
    34621 ttgcagcggc attacttatc cgcaaggtgg ctggctgtgt ccagcagaac tgacccgtaa
    34681 tgtgctgaaa ctggcgcaac agcagggttt gcagattcat tatcaatatc agttacagga
    34741 tttatcccgt aaggatgacg gttggttgtt gaattttgca ggagatcagc aagcaacaca
    34801 cagtgtagtg gtactggcga acgggcatca aatcagccgt ttcagccaaa cgtcatctct
    34861 gccagtatat tcggtggccg gtcaagtcag ccatattccg acaacgccgg aactggcgga
    34921 gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg cgaatcagca
    34981 ccattgtatt ggtgccagtt atcatcgcgg cagcgaagag acggcgtaca gtgatgaaga
    35041 tcagcagcag aatcgccagc ggttgattga ttgtttccca cacgcgcaat gggcaaaaga
    35101 ggttgatgtc agtggtaaag aggcgcgctg cggtgtgcgt tgtgccactc gcgatcatct
    35161 gccaatggta ggcaacgtgc ctgattatga cgctacactc gtggaatatg cgtcgctggc
    35221 ggagaagaaa gatgaagcgg taagcgcgcc ggtttatgac gatctcttta tgtttgctgc
    35281 tttaggttcg cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg cagcacagat
    35341 gagtgaagaa ccgattccga tggatgccag tacgctggcg gcgttaaatc cgaataggtt
    35401 atgggtgcgg aagttgttga aaggtaaagc ggttaaggcg gggtaatctg ctttggcatt
    35461 gtttgtcgga tgcggcgtaa acgccttatc cgacctacgc gttacctgta ggcctgataa
    35521 gacgcgataa gcgtcgcatc aggcatggtg ctccagacgt cacaacttac tgctgtgagg
    35581 cctgctgaaa caggtgttcc cacatatcgg taaccagcgc ctggtcacgc ggtgacaatt
    35641 cacccgcgcc aatggctttt tccagactct ggctaacggt tgtatgtacc gcctgagcgg
    35701 agtggtcgtc accactttcc agttctgcga tggctaatgt caggtggcca cgcaaatacc
    35761 cactggcaaa taactcatca tcactggcat ggtcaaccat accgtcgatt aatgccagaa
    35821 tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg cgctggcttc
    35881 agctgagcgc ttccggccac gggaattgtt ccgccgtaag ttccggcgtg tgataataat
    35941 tttgtagtgc tttaatgaag cgtgccggac gttcgggaat gccgtttgcc agataatcca
    36001 tcacctgcgc atgtacccgc cgttgaaaca ccacgcggtc cggttcgaaa tccccttcca
    36061 gattgtcgca gctaacatta aacggatatc ccgccgccac gcagaacaac cagtccagcg
    36121 cctgcggctt cacttcaaca tcttcaaact gactttgcgt ctgggcatcg cgtccgtccg
    36181 ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc tttcccggca atacaccagt
    36241 gcgaaatctc gtggatggcg ctggcataaa agccatgagc gaagacgatt ctgttatacg
    36301 gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc agacgggtat
    36361 taaattcatc ggcaaagcag ctattaaaaa tctcaatcaa ctgctcatag tggtgtgtac
    36421 tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat cgtaaagtaa
    36481 tttggcactc atcaccgccg agacgataac gatcatcggg cggatcagct tttgtccttt
    36541 gctcaacacc agtcttgaac ccatgcgcgc gccgaggaac tgccccacca gcatcacaaa
    36601 gcccgtcgcc caaatcactt tgccgccgag aataaacagc agcagaccgc caatgtttga
    36661 cgttgcgtta agtaatttgg cgtgagcagt ggctttggcg aggttgaatc cgcacagcgt
    36721 aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aagaatccat cgtaaaaacc
    36781 gacgcagccc cccgccacca gcgcaaatgg cagtccatac atccgacgct ggcggtcttc
    36841 ttcacccagc tttggcatca gcaagaaata gaggccgata caaatcacca gaataggcaa
    36901 aatctgccgt aagacatcgg cctgaacata ctgcaccagt agcgcgccgc tcattgagcc
    36961 gacaaaggtc atggcgatat tgagtttttg atcgcttaaa ctaaccactt tacggcgaat
    37021 aaagtagata gtggcggaaa tagagccacc gcaggcttgc agtttattgg ttgccagcgc
    37081 gttagcggga gacatccccg ccgccatcaa tgccggaatg gtgagtaacc caccgccacc
    37141 ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc ccagcagcag
    37201 cggggaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc atccagtagc
    37261 gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt tgttcccggt
    37321 tttggaggtt caaaccagct ttgcagttct gccccgcaac catcgcctgg tggcggtaaa
    37381 ggttgatcct cacactccag actatcggca ggacaacgta atcgtacatg catatgcgcg
    37441 cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc cgcatcaagg
    37501 caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt gtcctgggcg
    37561 gcgagtttga tcaagctgaa aatttctggc ttccacagcg cggggacaac gtgtttaccg
    37621 tcgcgggaaa ctaaatctaa cgcttgcggg cgcaagagct gcgcggaggt ccagcgtgtt
    37681 ttcggcagtt gcaggaagat atcgacatcc agtccggtct ggtggctggc gtgaccgccg
    37741 ttgaaacgac cgccagcagg catccccata tcgccaatca gcaccgtacc catgccaaga
    37801 ttgctcacct ggcgactcag acgctggata aacatcacca gatccgggtg cccgaaatag
    37861 cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa cgtgtcagca
    37921 ccgacgatac agccattaga aaaactgcct atcgattgtg cgctacccgg cacaggttgg
    37981 gttatttttt gccacggcgt cgctgccagg ctggcgctac tggcaagcag agtcagcagc
    38041 gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac atcggcattt
    38101 tgcgcccgct gccgtagcag gtgatccatt aaaacgatcg ccagcatcgc ttctgcgatc
    38161 ggcaccgcgc ggatcccaac acaaggatcg tgacggcctt tggtgatcat ctcaacttct
    38221 tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg tttcagcgcc
    38281 atatgggcaa tgattttctg cccgctgctg ataccaccaa gaatgccgcc cgcatggttg
    38341 ctctggaaac cgtctttggt gatttcgtcg cggttctggc taccacgcag cgctaccacg
    38401 tcaaaaccat caccaatttc cacgcctttc accgcgttga tgctcattag cgcatgggcg
    38461 atgtcggcat ccaggcggtc aaagaccggc tcgccaagtc cggcgggtac gccactggca
    38521 acgacggtga ctttcgcacc aatagagtcg ccttcttttt tcagcgcgcg catcagttca
    38581 tctaacgcgt cgattttgtc cgggtccggg cagaagaatg gattttgctc gacctgagac
    38641 cagtctttga tttccagtgg aatgtcgccc atctgggtca ggcaaccgcg aatctcaatg
    38701 ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg catagcggtt
    38761 tcacgggcgg aagaacgtcc accgccgcga taatcacgca ggccgtattt ttgttcgtaa
    38821 gtgtaatcgg catggcctgg acggaagacg tctttaattg cgctgtaatc ctgagaacgc
    38881 tggtcggtat tttcgatcaa caagccaatg ctggtgccag tagtaacacc ttcaaaaaca
    38941 ccggagagaa ttttgacctg atccggctca cgacgctggg tggtatagcg cgatgtccca
    39001 gggcgacgac gatcaaggtc atgttgcagg tccgcttccg taagcggaat gcctggcgga
    39061 acaccatcga cgatgcagcc gagcgccagc ccgtgcgatt cgccgaaggt ggttacgcga
    39121 aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg tttgcgtgtt
    39181 tacttaatct ttataaatcg cgaaatgttc acgggccgcc agcagctgct ctttggtgag
    39241 cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa catccggata
    39301 ttgttccata agatgtacca tgctgttgcc gacttcacaa atcagcacgc catcatcagc
    39361 gaggtaatct gccgcgttac cgagaatgcg acgcgtcagt ttcaggccgt cggtgccaga
    39421 tgccagaccc agttccggct cgtgacggta ctcatttggc aggtcggaca tatcttccgc
    39481 atcgacatac ggcgggttag tgacaatcag gtcgtactgc actttcggca agtcgcggaa
    39541 cagatcggag cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga tgttctgttc
    39601 agcaaccgcc agcgcgtctg gagagatgtc caccgcgtcg acttccgctt ccgggaaggc
    39661 ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa tgtgctgcgg
    39721 ttgcttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg gcgagcgcgg
    39781 caccagcacg cgttcatcga cgtaaaattc atggccgcag aaccaggctt tgttggtcag
    39841 gtaggccacc ggaatgcgct cgttgacgcg gcggatcaca cgctcaacaa tgcggtgttt
    39901 ttcgctggag gtcagacgcg cggtgcgcat atcttccgga atatccagcg gcaagtagag
    39961 cgaaggcaac accagttgca cggcttcatc ccacgggtta tcggtgccgt ggccgtacca
    40021 gatgtttgcc gcgctgaagc ggctcaccga ccagcgcaac atgtcctgaa tggtttgcag
    40081 ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg catgatcgca
    40141 ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca cacgcggtga
    40201 gtgaggaaaa atacgtttta agcgatcgat tgtacggtga gtcgggtaaa ctgtaggaaa
    40261 attagaaata gagacagtta aatgaaaaag aaaacaacac ttagcgagga ggaccaggcg
    40321 ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat tgtccatcga
    40381 ccacaacgca agaaaatcag cgaagtaccg gtcaagcggt tgattcagga gcaggccgat
    40441 gccagccatt atttctctga cgagtttcaa ccattattaa atactgaagg tccggtgaaa
    40501 tatgttcgcc cggatgtcag ccattttgaa gcgaagaaac tgcggcgcgg tgattattcc
    40561 ccggagttgt ttcttgattt gcacggtctg acgcagctac aagccaaaca ggaactgggg
    40621 gcgttgattg ccgcctgccg ccgtgaacat gtgttttgcg cctgtgtgat gcatggtcat
    40681 ggtaagcata ttttaaagca gcaaacgcca ctgtggctgg cgcaacatcc ccatgtaatg
    40741 gcatttcacc aggcaccaaa agagtatggt ggtgatgcgg cattgctggt gttgatagaa
    40801 gtagatgagt ggctgccgcc ggaattgccc tgaatattta aaggtggacg agtctgtcca
    40861 tttagccttg aatactggcg tctcaaccct ggcgataatg aacgtaataa tcgggcatta
    40921 attgaaaaac gtaagcagct ttccgacagc cttctgtggg ctaatggcat tgccccttca
    40981 gaggggcttg aggatgacga cctgctgcaa gcggttatcg acgaattact cacccccgtc
    41041 gacggttatc tggcaccttt cgctcctgtg gtggcgaata ttctaaaaaa ctggatgacc
    41101 gcatgacata acggacaacg gggccagatg gccccgcatc gtgtgtggtg tatacgctac
    41161 agctcgttga gcgtgtactg gaagcggcag gtggaatcaa tcggctggcg gctgctggta
    41221 tcgacacgca ctaccatgtc gtgcagttgc ttgtcagtga tgggatgctg gttgggtatg
    41281 accgtcactt tcgaactgtc acagccggag gtggagaagg tgatcatctg cgccacaaag
    41341 cggcagtatg cgcctcgatc gtccatcacg ctgccgtcac agagcttgcc gctttccatc
    41401 aaaaaggtgg gcagcgtttt ctcaatcgtt ccacccgtcg attgcagcga cagcactttc
    41461 gaatggacaa cataatcttc actccacgaa atatcataag cggcgacgga gacggggcat
    41521 gatgagttgt gaactttggg gccaatatat agaccaacac catctaccga agagatattg
    41581 ctgtcagtat atgtcgagat caaccacggg caaccgtgca cctcaccatc atagtacact
    41641 ccgaggctgg cgttgtaaca gatccaatca gatttactcg accgagcatc atacgcgtca
    41701 agaccaacac aggtagattt tgaagcatca ttgagctcct ccattttcaa cgtcggtgcg
    41761 gcggtactcc ctgtccagga aactaaatct aaattctcag cttgaagcac aatactgtgt
    41821 tgcttataaa cgccatactc aaccccttca ggggtcagaa tttcaagata ataacgtatc
    41881 tgtgttagat accccgaata tgccaccggg gtcaccagtg tcgcaatttt cttcgctgcc
    41941 agactttctg ctgcgcaagt cagcatgacc aggcagcaca accatcggaa tattttcatt
    42001 ttatatgtac tcaaatgtcg cagtcaggga ggcggtaaaa tcccccatcg tcacttcccg
    42061 gccgcttttc gcctggagcc aggcattaaa attcagattg ttattgccgt tactcagcaa
    42121 aaaggtcgcg ccgctggtgt tgttgatcgg caccgccgtt ccgtcggttt tttccatgcc
    42181 aatccccacg ccctgcgccc ctgaggacgc atccagcgcc agaaagccgg gctgttcact
    42241 gtcttctgta ccggtcagcg tcacccggac ttcatactga gctggccctt tgcaatcttt
    42301 cagctgaaac actaccggca cccgcgccga ctgcccggtc accatcaggt ctttgcggct
    42361 aacggtgggg aaatgcacct ccgccagcga ctcgccctga acgactaacg tgcaggactt
    42421 gctgagtaaa ttgccgtaga aatgcaggtt gtcagccgcc tgaacatggg gcgtcagcag
    42481 caccgccgcc agtgcagccg cgcagtgaga tatccgtttt aggttcattg gtaatccacc
    42541 tgtaatgttg cgtaggcaga aaaggttccg tcgctgagcg ccgcatcatt ggctttcacc
    42601 ggcacagctt gcagcgttgg cggcgttgac gggtcaattt tcaacggtgt tcccagctta
    42661 aacggctggc cgttttgctg tagctcaatc cccaggccgc taacgtccgt ttcaatggcg
    42721 gaatcatcat aaggggtttg gctgcccgtc caggtcaggc tcatctccca ggcgtcgtcg
    42781 cgcacatcgg ggtcacatgt gatggtgtaa ggcacatcct gacggaaatt atcgccgtta
    42841 atgttgtcga taatgacgtt gcgaaactca acttcgatcg tcttaccatc gctgatagta
    42901 caggcgggcg gcgaaaccag cgtgccatga aaagtaacgt cgttaatcgc ggcctgcgcc
    42961 aggccagcaa aaccgtaaat taagcaccat gcgagtcgtt tcattggtag tccaccacca
    43021 tggtcatggt cgcattaaac tccgatgcca tcagttccac accgctctgc ttcaccggaa
    43081 cggcttcgaa gtctggctgt gtattgatgt tgaacggcag ccagctactg ttacccactg
    43141 ccaccagcga atggtcagcg gcattctgaa tacgaatgcc aaaaccgctg attccagcac
    43201 tcagcaccgt ttcgccgttg atggtcgtgg tggtggcctg aagctgcatt tgcaggtcgt
    43261 cgctgacact gtttgagcag ttcagggtgt agcccactgg ctgacggtag ttggtgccat
    43321 caatgctttt aataatcaca ttgccgaact ctaccgcgct tccctttacc gaacagggcg
    43381 gcggcccatc caccagcacg cgcaacgtca aattgacgat tttgccgccc gccattaccg
    43441 gtgaaacgat cgcgccacag gccagcagta gcgcggcaga tctggtcaga aagaacgctt
    43501 tcatgttgct tctcccgtta tcaacctttg ctgctttgat cttcatccac cttgcaggtg
    43561 ttcccttcac aacggaagat cagcggcatg cgcgcgccgt agtcgttgat ataggtcagc
    43621 accggggcgc tcgccaattt tgcctttaac ggttgtgacg ttttcggtgg catcaccagc
    43681 ggtttaaacc cggcggcagt cagccctttc ttctgtgtgc tggcatcgct gatgatgacg
    43741 tggtacggcg tcgggttatt gacggtatag tcctggccac tgcgcgtgag tgtgacctta
    43801 aactgccacg gtcttctcat gtcaatcttc tccagcgcct ttggtcgcca gaacagcttg
    43861 atgcgcgttt gcagcgcaat ttgcagggtg ttggctttcc cggactttgg cgggatctca
    43921 cgcacgttgt agtagaacag gctttctcgg tcggacggca gtgtgtgaat gtccggcagc
    43981 gcctgaatct tcacctggcc gttcatcata gcgtcaatac gctgcaccgg cggcagtact
    44041 gccagcggcg aagtgatttt gttgcccttc tcgtcttcca gccagctttg cgccaggtaa
    44101 ggcagctttt cgttgttgtt acgcaacgtc acgctgatcg atttatcgct ttcgttaaac
    44161 accagacggg tgcgatccgg ggtgaccgat gcatgcgccg tcaggctggc ggtggtaagc
    44221 agtgccatca gcgctgtgct gacgagcatt ggacaggata atttataaga cattgatcgt
    44281 tccttaatca gattattgag ttgctgaaat tgacgtcggc gcttccgttg gtgtgacttg
    44341 ccgcgtctgg tcctgaataa caggcttaat ttctggcgct gaagaatcag gggctgccgt
    44401 tcccttttgt tcacacggca acaacagacc gctgaacaaa tcggcaggta gcggcttcgg
    44461 caatacgatc tcgcattgtg cgctgccttc ccagaacacc atcatatgct cgtccgcatt
    44521 gacccccgcc agatagacat tgccttcatc atccacaatg ccaacctgct gctgctcgtc
    44581 gtttttcact tccgcgccaa acggcgggta gctaccgtca cgcaaccgca gtaccgccat
    44641 cgctttttgc ccgctgatca ctttgaattt acgatagccg attgcgcctt ccgtcagagt
    44701 cgcctgcacc accgactggg tggcttccgc atcttccggc aagttgttta aatctatata
    44761 cgcctgattg cgatagtagt tgttaatatc ggcgaccacc gccttgccaa acatattggt
    44821 gtacaccggc gcgccgttgc tttcgaccgg cacgttggca atcccgtcgg cgtcaatcag
    44881 caggcgcgta ccgcccatgt tctgggtacg gtgcagcgcc ccaccatgcg cggtcagcgt
    44941 tgccccgccc tgtaaggcga tccccgccga acggtattcc ccttcatggt agttggcgct
    45001 gagatcaacc ttcgccagcg aaccgtcgtg gctcagataa ccatccacgc tgccgtgctg
    45061 ctcgctggtg ccaacgttaa cctggtagtg cgttgcgtca tcgacgcgct taaagtaacc
    45121 gacctggctg ctgtccgacc cgctgccgta ggaaccgttg taggtcacgg tgctgctgtc
    45181 gctccacgga atgctcatac tgaggtacat ccccttatcc gcattatcgt catattcgta
    45241 gcgatagccg gtcaccgaga tgctcatgtt gcgaatgctc cccatattaa aatagtggga
    45301 aaacatcagg ttatagtttg tctgttccgg gcgatcccag taagtacgat gggagtagtt
    45361 gagatagatc gagacacccg cagcggcaaa gttctggtta taggtgatcg tatacatctc
    45421 tttgtcgtta ccggtccgcg ccatgtccga ctggttcgcg tccagatact cgctcatggt
    45481 catgaagttc ttttcagaaa aacggtagcc tgcaaaggtg acgcggctgt taagttcgtc
    45541 aaagtctttg gcatagctca cgcgaaagga gttgccgtcc agttttcctt tgccgtatgc
    45601 gctgtcatga tccaggttga cgtgcgagtg agtcacatcg aacgccagcg cgccaaactg
    45661 tgcgaggtcg cgccccaccc ccatcgccgc cgactgatag tgtttatctg ccagcgcacc
    45721 accgtagagc gaccagccat ctgccacccc ccatgacgct tctccgccgg agaaaaagcc
    45781 gccttcggtc ttgtgcttcc agtcttccgg acgccccatc atcactttgt aacgcacctg
    45841 cccctggcgc gtgaggaatg gcatagatgc ggtcgtaacg tcatattcct gcacctgacc
    45901 attctgttct tcaacgcgga cgtgcagtgt gccggagacg gagtcgccga tatcctgaat
    45961 gcggaatggc ccggcgggaa cctgggtttc gtaaagtacc cggcccatct ggctaatggt
    46021 gacttttgca ctgctgtgcg ccacgccgga aacatccggt gcatagccgc gcaggttggg
    46081 cggcagcatc tggtcatcgg tgctgacgct gctgccgata tagttaaagc cgtcgaaaat
    46141 atcggaattg agataatctt cccccagcga cagcttcgct tttaaagagg gtaaggcccg
    46201 ccaggcgtaa taacggctcc agtcccagtt tttgctcgtt gtactgttac tgctgtcatc
    46261 gtcgtcatcg ttgctgcggg tgtgctgata atcactttgc cagtctgcac ggaaacgcca
    46321 tgcccccagg ttcgccccaa cggtaccgtt gccgctgata tcatgtgagt cctcgccccc
    46381 ctgctcctga tggcgagttt gcgcattgag gctgtagtcg gcaatcaggc caggaatacc
    46441 gtcgtcccag cgtgagggag ggtcccagtc gctgctggta tattcgaggt aagcctgtgg
    46501 cactgtcagc agcaacgccg actggcttaa atcattctcg acttccatcc cgtctaattg
    46561 acctggttta aggcattcat cgttgtgcgt ccactgcagg ctttttgcta tcccttcttt
    46621 cagccccagc gccgccacta actcaggtgt caggcaggca tagttttttg ttggatcgtt
    46681 ttcagagacg taccagttaa tgtcgtattc atcagaaagg ggttgtttat ttataaatac
    46741 acgtaaatta tatttacccg cgtcgacata cccttttttt gaaaacttac cgagatcaat
    46801 tttcgtatcg ccctttaact caaggaaacg ggggtcaaat tgaattgaat catcagccca
    46861 tgcattgact gacccaccag agattgccag cgcaatatag caggcgattc cccgcagccg
    46921 aaaatttgag tgattaggca tacacatatc cgtttgtgaa acagtcgtaa atctgttgcg
    46981 tgttgaataa ccccacagca gaacgctgtg gggttgtcac atcagagata ggtaatctgg
    47041 aatgtggtca gggtttcgaa ctggccgaga tccggcgcat cggtttcacc caccagccag
    47101 gctttaaagt tcagcgtttg cttgtcttta cccttgctgg ttgaagtatc catcatgatt
    47161 ggctgaacaa tcttttcgcc cactttataa cctttgccat gggtatcgcc gattaccagg
    47221 ctcacgtggt tcatggcaac accgctgtca acattgaata acgcatagtt atccgtgttg
    47281 gctggccccg cgtagttagt accggtgaac gtggtctcca tgtttgtttc agtggtaaac
    47341 acgcaatcct gtaaacggat ctggaaatct ttcggtgtgg tagtgccacc gccggtcagt
    47401 ttagccgcac cgatgtcacc catatcgact tccagtttgt gatcgtctgg aacgatagag
    47461 cacggggagt cttcaatggt gccgtagaag cgcgcggtac cgttgttgcc tgctgcaaaa
    47521 gcagaactgg aaacaactgc gcccatgacc atcgccgcag caatggctgt tttggcaaac
    47581 ttactcatta tttgtcctta aatcattaag agttataata taagcacctg acataaaatt
//
LOCUS       DADSZD010000016        45458 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.016, whole genome shotgun sequence.
ACCESSION   DADSZD010000016 DADSZD010000000
VERSION     DADSZD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.016"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..198)
                     /locus_tag="J1Z28_000822"
     CDS             complement(<1..198)
                     /locus_tag="J1Z28_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2574 family protein"
                     /protein_id="HBA7715679.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVS"
     gene            complement(461..1570)
                     /gene="apbC"
                     /locus_tag="J1Z28_000823"
     CDS             complement(461..1570)
                     /gene="apbC"
                     /locus_tag="J1Z28_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBA7715680.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1702..3735
                     /gene="metG"
                     /locus_tag="J1Z28_000824"
     CDS             1702..3735
                     /gene="metG"
                     /locus_tag="J1Z28_000824"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBA7715681.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3876..7679
                     /locus_tag="J1Z28_000825"
     CDS             3876..7679
                     /locus_tag="J1Z28_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBA7715682.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTQGQSQVKSFAD
                     AVAAEKAELKLIAEKTKKGYVEGNSANVHIPPVTKESIPSEVKTAVVSKNKHPWLADD
                     AAIPVTDDINRFAFPHRSRPREINYLHKDGQIWKRIANNTRAYDPDNNYRSYPENWQQ
                     AFAELQSRILDNQQTGSAQSDAALLWSFWNSYSADELVDDLVIRCGLESAVEIALFAL
                     QLKYKPVKGAVTTIIPPGHKAESLPSWHQRLCHHLSLASEDEWQRCVNKVLTAIPTLS
                     PARQPFAALLLPERPDIANAMALRYADQNVPAMTWLRLVVTDDAALTAAEKYDFPPLY
                     RDFRAYLAMLLANNGVSGVSRILTEFVEEHSDNPTYLFERISETEDLVKWLWKTNHPD
                     AIQILILGVIGKKKHLEYLSKACQKHSAAAIAAYATLLAIHEDKEWHKALVKLITITP
                     ELVCDVIPWVNAKAAGILSECRPQSVAEECEYATADMLPQLFVAPPWVINKKKNVIPV
                     FDLPVLPIPAVTDITPGITELISHTDISRFYEIAQYQASQQTLFTDLPLIEKESWETS
                     FIPFTPEQQILWQLGFNEWLHCEDDLHEKKYIPQSAVDALLRFDFPALKAEFAKYHNN
                     ANKSWNLSALCYLPGQQAISFLNQIIIEEQYSGEKEILAVFGSTAIPAFMTCLQRDHQ
                     RLWIFTLFIGASELALPMAQRLQKKMAYKDAVNWLANNPRHAAAGLLPLALGKPCQNR
                     EYARQALLLLVKLNQRETIEEIAQGYNQPDILAALATLFDSDPLEEHPAKIAPLPGFY
                     QFTLWRRPRLKSNNLPLPDDAMRHLGTMLSFPRDITAYAGLDIIRETFTRESLADFGW
                     DLYTAWTEAGAPAKENWAFTSLGILGNDDTARKLTPLIRAWPGESQHKRAVYGLDVLA
                     SIGSDIALMLLNGIAQKIKFVALQEHASDRINMVAENRGLTMAELEDRLAPDLGLDIN
                     GSLILDFGPRKFTVGFDETLKPVVRDANGKVLKDLPKPNQSDDKTLATDAVNLFKQLK
                     KDVRAIASQQIDRLEQAMCQHRRWTAEQFRLFLVEHPLVRHLTRRLLWGVYTEENTLI
                     ACFRVAEDSTYSDAQDELFTLPAGNIGIPHVLEISPESAAAFGQIYADYEKLPPFRQL
                     DRGYYHLTDNERDTHELIRWQGRLCQAGRIVGLERRGWQRLEESGSVYAMRKSTPYGA
                     LELETEHFSLIYGETGYSDLLPVESVKITSPGERYSTQPSLTFSALDAITASELINDI
                     ESLFD"
     gene            7692..11486
                     /locus_tag="J1Z28_000826"
     CDS             7692..11486
                     /locus_tag="J1Z28_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HBA7715683.1"
                     /translation="MKTYIYQDEKSHKFWAVEQQGNELNISWGKVGTNGQSQVKSFAD
                     AAAAGKAELKLIAEKTKKGYVEQAENAPSKHTRTVTKSLELSDFSTIIQEQPSVTPEI
                     YVADKNTDVILPWLAKDIAVVFPLEVVPTTLSHRRFPGDPVTPVDKLTQLRRLACGVS
                     QRDNKTATFDFSACTLEWQNFIAQAIDLINSPKTTKLPSPVMAVLTALELHCTRYKER
                     EDVMDQIIQEGGLEYATDVIIHLQQISIEWDYVNNNIVFLSSGISPDYLQQYSSFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPGIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     VASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAALAALAELLVQK
                     EENSWRIMLMTMLISQPTLAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAIV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPISYLTEEISNQLLAKYIWYSKHITVSHEESTA
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLISEFKVFHSPTGHYWHLGILTTL
                     PLEKAVRAWNALTLSPHTDTEYAMLHFGFKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRQDARSWLLKYPQHAITGLLPAALGKTGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPTFYQPSLWTRPLLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCTTDSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     ITQKLKFKALQERAKEKIANIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDASGSRLKDLPKPNKSDDESQANDAVNRYKLLKKDARTVAAQQVAR
                     LESAMCLRRRWSPENFQLFLVEHPLARHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGIPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWTGRKCPSGRVMGLANKGWIKGTPQDAGWIGWMINPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            11496..15128
                     /locus_tag="J1Z28_000827"
     CDS             11496..15128
                     /locus_tag="J1Z28_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBA7715684.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEETCWQRCADKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDQKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPWLSTQAQAVVNNYLSTSPKPVIDGTDNSQMPEILVSPPWRSKKKMTAPRLDLASL
                     ELTPQVYWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYPGRVEEIMNGWSSPEAQLAEQALRSGHVEVLINIWEND
                     SYSRYRREKSIWNLYLLAQLPREMALPFWLRINEKKHLSAGEDYFLSIFGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKSSDNSEAALLALRLLYEQGHGELLQTAANRWQRTDVWPALEHLLKQSPIEIYPA
                     RMPKAPDFWHPQMWSRPRLIINNQLITDDALEIIGEMLRFTQGGRFHSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSTR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRTDDDQLKAPE
                     ALARLKGLKKDTTQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTEGMRSEFAQLF
                     ADYEIMPPFRQLARRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            15189..15505
                     /locus_tag="J1Z28_000828"
                     /pseudo
     CDS             15189..15505
                     /locus_tag="J1Z28_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16285..17373
                     /locus_tag="J1Z28_000829"
     CDS             16285..17373
                     /locus_tag="J1Z28_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBA7715685.1"
                     /translation="MSPQNNHLQRPPAAVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDEAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            17384..19663
                     /locus_tag="J1Z28_000830"
     CDS             17384..19663
                     /locus_tag="J1Z28_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715686.1"
                     /translation="MSEPLIVGIRHHSPACARLVKLLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDESPDTQDDSQTLLLRATCMDNSDTLWDHLFEDESQQTALPSALARYFA
                     QLRGDSPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVINPDTDPILVTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERRSQAIHAPALDRGATLGAL
                     MRLEHPNASAETALTMLAQLSPAQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQAIAYH
                     QQLELQALASLQHWGVFHV"
     gene            19656..20792
                     /locus_tag="J1Z28_000831"
     CDS             19656..20792
                     /locus_tag="J1Z28_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA7715687.1"
                     /translation="MSELNDLLTPRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDMAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            20789..22792
                     /locus_tag="J1Z28_000832"
     CDS             20789..22792
                     /locus_tag="J1Z28_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715688.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVALAVQAFVDAKTQQAEFTHLIWQMRSEHATSSDDPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCAQKMARRDSVGEVPPVPWRTVVGADIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADGELLFATRNRLSSVVPLSPDAWQMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            22917..23378
                     /locus_tag="J1Z28_000833"
     CDS             22917..23378
                     /locus_tag="J1Z28_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBA7715689.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(23419..23889)
                     /locus_tag="J1Z28_000834"
     CDS             complement(23419..23889)
                     /locus_tag="J1Z28_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBA7715690.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(23936..24655)
                     /gene="yehT"
                     /locus_tag="J1Z28_000835"
     CDS             complement(23936..24655)
                     /gene="yehT"
                     /locus_tag="J1Z28_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBA7715691.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(24652..26337)
                     /gene="btsS"
                     /locus_tag="J1Z28_000836"
     CDS             complement(24652..26337)
                     /gene="btsS"
                     /locus_tag="J1Z28_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBA7715692.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            26559..27290
                     /gene="mlrA"
                     /locus_tag="J1Z28_000837"
     CDS             26559..27290
                     /gene="mlrA"
                     /locus_tag="J1Z28_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBA7715693.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGD
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            27350..27457
                     /locus_tag="J1Z28_000838"
     CDS             27350..27457
                     /locus_tag="J1Z28_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBA7715694.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(27438..28169)
                     /gene="yehW"
                     /locus_tag="J1Z28_000839"
     CDS             complement(27438..28169)
                     /gene="yehW"
                     /locus_tag="J1Z28_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBA7715695.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGQQPAIIALILYGVLPVLQATLAGLGSIDASVTEVAKGMGMSCSQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(28174..29100)
                     /gene="yehX"
                     /locus_tag="J1Z28_000840"
     CDS             complement(28174..29100)
                     /gene="yehX"
                     /locus_tag="J1Z28_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBA7715696.1"
                     /translation="MIEFSHVSKLFGTQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALEERMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(29093..30250)
                     /gene="yehY"
                     /locus_tag="J1Z28_000841"
     CDS             complement(29093..30250)
                     /gene="yehY"
                     /locus_tag="J1Z28_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBA7715697.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(30257..31174)
                     /gene="osmF"
                     /locus_tag="J1Z28_000842"
     CDS             complement(30257..31174)
                     /gene="osmF"
                     /locus_tag="J1Z28_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBA7715698.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTVWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQIAQWLQPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLTQKGWTK"
     gene            complement(31364..33661)
                     /gene="bglX"
                     /locus_tag="J1Z28_000843"
     CDS             complement(31364..33661)
                     /gene="bglX"
                     /locus_tag="J1Z28_000843"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBA7715699.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            33857..35572
                     /gene="dld"
                     /locus_tag="J1Z28_000844"
     CDS             33857..35572
                     /gene="dld"
                     /locus_tag="J1Z28_000844"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBA7715700.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEANTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(35611..36543)
                     /gene="pbpG"
                     /locus_tag="J1Z28_000845"
     CDS             complement(35611..36543)
                     /gene="pbpG"
                     /locus_tag="J1Z28_000845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBA7715701.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNSTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(36717..37304)
                     /locus_tag="J1Z28_000846"
     CDS             complement(36717..37304)
                     /locus_tag="J1Z28_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBA7715702.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            37474..38052
                     /locus_tag="J1Z28_000847"
     CDS             37474..38052
                     /locus_tag="J1Z28_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7715703.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(38172..38933)
                     /locus_tag="J1Z28_000848"
     CDS             complement(38172..38933)
                     /locus_tag="J1Z28_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7715704.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     RKVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(38986..40512)
                     /gene="mdtQ"
                     /locus_tag="J1Z28_000849"
     CDS             complement(38986..40512)
                     /gene="mdtQ"
                     /locus_tag="J1Z28_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311052.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBA7715705.1"
                     /translation="MLLMYCHAKLKNISQHTVISAHLFLPDYSPMNRDSFYPAIACFP
                     LLLMLAGCAPMHETRQALSQQTPAAQVDTALPTALKNGWPDSQWWLEYHDNQLTSLIN
                     NALQSAPDMQVAEQRIQLAEAQAKAIATQDGPQLDFSADMERQKMSAEGLMGPFALND
                     PAAGTTGPWYTNGTFGLTAGWHLDIWGKNRAEITARLGTVKARAAEREQTRQLLAGSV
                     ARLYWEWQTQAALNTVLQQIEKEQNTIIATDRQLYQNGITSSVEGVETDINASKTRQQ
                     LNDVAGKMKIIEARLNALTNHQTKSLKLKTVALPKVASQLPDELGYSLLARRADLQAA
                     HWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTLPIFD
                     SGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIERDAL
                     RVVGLAQARFNAGLIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGGGYKR
                     "
     gene            40646..40729
                     /gene="yohP"
                     /locus_tag="J1Z28_000850"
     CDS             40646..40729
                     /gene="yohP"
                     /locus_tag="J1Z28_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBA7715706.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(41117..42067)
                     /gene="dusC"
                     /locus_tag="J1Z28_000851"
     CDS             complement(41117..42067)
                     /gene="dusC"
                     /locus_tag="J1Z28_000851"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBA7715707.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMVGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            42306..42704
                     /locus_tag="J1Z28_000852"
     CDS             42306..42704
                     /locus_tag="J1Z28_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA7715708.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            42701..43396
                     /locus_tag="J1Z28_000853"
     CDS             42701..43396
                     /locus_tag="J1Z28_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBA7715709.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            43526..44410
                     /gene="cdd"
                     /locus_tag="J1Z28_000854"
     CDS             43526..44410
                     /gene="cdd"
                     /locus_tag="J1Z28_000854"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBA7715710.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            44560..45279
                     /gene="sanA"
                     /locus_tag="J1Z28_000855"
     CDS             44560..45279
                     /gene="sanA"
                     /locus_tag="J1Z28_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBA7715711.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            45282..>45458
                     /locus_tag="J1Z28_000856"
     CDS             45282..>45458
                     /locus_tag="J1Z28_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBA7715712.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMV"
BASE COUNT        11245 a        11969 c        11397 g        10847 t
ORIGIN      
        1 agaaacctga tgacgggtat caacattata cgttaactga ccacaatgct gttgaggttg
       61 accattaacc atagccatac tgcatgttgg gggactgatc ctaccattaa tcgttaatgt
      121 agcagtttct gaagagaacg cgggagaagc caatccatag gcaagaaaaa taattcctga
      181 cagccagtac ttattcattg aacgttatcc ctgtagtaaa ggttatgcct ggaaagaatg
      241 agtgcataac aaactatagc tgtacatcca ctacacagcc acgaaggatg ataatgaagc
      301 atcgccggta cgatcaatcg actttgtaga gtttcggacg aaggtccgca gaatattcgc
      361 agcattaaat aagtgttcat taatgttgca aaaacaaata caccgcatca atgtaacatc
      421 tctgcggtgt atgaagaata accagaatgg ttaatacgcg ttagaccgcg cggaaggaaa
      481 tctcgcctgg aatgacttca ccctgccagt agagctgagc tgcaacgcgg tcagccagct
      541 ggcggtagat agcggtaaat tcgctctcag gacggctgat aaccgttggc gtgcctttat
      601 cgagatcctc acgtaaggag atgtgtaacg gcatctgccc cagcagctgc gtgtggtatt
      661 tctcggccag tttctctgca ccaccggtac cgaaaatcgg ttcatggtga ccacagttac
      721 tgcaaatatg cacactcatg ttctcgacaa taccgagcac cggaacctcg actttctcga
      781 acatcacaat gccttttttc gcatcgatca atgcgatgtc ctgcggggtg gtgaccacga
      841 ctgcaccggt tactggaatg ttttgtgcca atgtcaattg aatgtcaccg gtacctggcg
      901 gcatatcaag caccagatag tcgagatccg gccacaaggt ttcctgcaac atttgcatta
      961 acgccttgct cgccattggg ccacgccaca ccatcgcatt gtcgtctgtc accagataac
     1021 caattgaatt agttgccagg ccatgagaca taatcggtgc catatgggta ccgtccggcg
     1081 aagttggacg ctgattttct gcgcccagca tggtagggat tgatgggccg tagatatcgg
     1141 cgtccagaat gccgactttc gccccttccg ccgccagtgc caacgccagg tttaccgccg
     1201 tggaggattt acccacgcca cctttaccgg aactgaccgc gataatgttc ttcacgccgt
     1261 taattcccgg ctgattcttc acgcgtttca gagtggctat gttatgcgac agtttccagt
     1321 cgatagcttt cgctccggtg atgcgcagca gttcggcgct acattgctct tttaactctt
     1381 cgaacgcgct atgccagacg aatggcataa ccagttccac atgcagcgtg tcgtccatcc
     1441 aggcgacatg gtgcaacgct ttcagcgttg tgaggttatg cttcagggtt gggtgctgaa
     1501 aattggccag tgtcccggcg accattgcgc gcaaggcttc tggtgatttg gcctgggatt
     1561 gttcgttcat cccgactcct tttttgtata gataaaccat cagttgatag tttacctgaa
     1621 gaatatagag aagtacttac ttaacatttt cccatttggt actatctaag cccttttcac
     1681 tattaagaag taatgcctac tatgactcaa gtcgcgaaga aaattctggt gacgtgcgca
     1741 ctgccgtacg ctaacggctc aatccacctc ggccatatgc tggagcacat ccaggctgat
     1801 gtctgggtcc gttaccagcg aatgcgcggc cacgaggtca acttcatctg cgccgacgat
     1861 gcccacggca cgccgatcat gcttaaagct cagcagcttg gtatcactcc ggagcagatg
     1921 attggcgaaa tgagtcagga acatcagact gatttcgcag gctttaacat cagctatgac
     1981 aactatcact cgacgcacag cgaagagaat cgccagttgt cagaacttat ctactctcgt
     2041 ctgaaagaaa acggttttat taaaaaccgc accatctctc agctgtacga tccggaaaaa
     2101 ggcatgttcc tgccggaccg ttttgtaaaa ggcacctgcc cgaaatgtaa atccccggat
     2161 caatacggcg ataactgcga agtctgcggc gcgacctaca gcccgactga actgatcgag
     2221 ccgaaatcgg tggtttctgg tgctaccccg gtaatgcgtg attctgaaca cttcttcttt
     2281 gatctgccct ctttcagcga aatgctgcag gcatggaccc gcagcggtgc gttgcaggag
     2341 caggtggcaa ataaaatgca ggagtggttt gaatctggcc tgcaacagtg ggatatctcc
     2401 cgcgacgcgc cttatttcgg ttttgaaatt ccgaacgcgc cgggcaaata tttctatgtc
     2461 tggctggacg caccgattgg ctatatgggc tctttcaaga atctgtgtga caagcgcggc
     2521 gacagcgtaa gcttcgatga atactggaag aaagattcca cagccgagct gtatcacttc
     2581 atcggtaaag atatcgttta cttccacagc ctgttctggc ctgccatgct ggaaggcagc
     2641 aacttccgca agccgaccaa cctgtttgtt cacggctatg tgacggtgaa cggcgcgaaa
     2701 atgtccaagt ctcgcggcac ctttattaaa gccagcacct ggctgaatca ttttgacgcc
     2761 gacagcctgc gctactacta caccgccaaa ctgtcttcgc gcattgatga tatcgatctc
     2821 aacctggaag atttcgttca gcgcgtgaat gccgatatcg tgaacaaagt ggttaacctg
     2881 gcgtcccgta acgctggctt tatcaataag cgttttgacg gtgtgctggc aagcgagctg
     2941 gctgacccgc agttgtacaa aaccttcact gatgccgctg aagtgattgg tgaagcgtgg
     3001 gaaagccgcg aatttggtaa agctatccgt gaaatcatgg cgctggctga cctggctaac
     3061 cgctatgtcg atgaacaggc tccgtgggtg gtggcgaagc aggaaggtcg cgatgccgat
     3121 ctgcaggcga tttgctcaat gggcatcaac ctgttccgcg tgctgatgac ttacctgaaa
     3181 ccggtactgc cgaaactgac cgagcgcgca gaagcattcc tcaatacaga actgacctgg
     3241 gatggtatcc agcaaccgct gttgggccat aaagtgaatc cattcaaggc gctgtataac
     3301 cgcatcgata tgaaacaggt tgaagcactg gtggaagcat ccaaagagga agtaaaagcc
     3361 gctgccgcgc cagtaactgg cccactggca gatgatccga ttcaggaaac catcaccttt
     3421 gacgactttg ctaaagttga cctgcgcgtg gcgctgattg aaaacgcaga gtttgttgaa
     3481 ggttctgaca aactgctgcg cctgacgctg gatctcggcg gtgaaaaacg caatgtcttc
     3541 tccggcattc gttctgctta cccggatccg caagcactaa ttggtcgtca caccattatg
     3601 gtggctaacc tggcaccacg taaaatgcgc ttcggtatct ccgaaggcat ggtaatggct
     3661 gctggtcctg gtgggaaaga tatcttcctg ctaagcccgg atgccggtgc taaaccgggt
     3721 catcaggtga aataatcccc cttcaaggcg ctgcatcgac agcgcctttt ctttataaat
     3781 tcctaaagtt gttctcttgc gattttgtct ctctctaacc cgcataaata ctggtagcat
     3841 ctgcattcaa ctggataaaa ttacagggat gcagaatgag acactttatc tatcaggacg
     3901 aaaaatcaca taaattctgg gcggtggagc aacagggaaa cgagttgcat atcagttggg
     3961 gaaaagttgg tacccaaggg caaagccagg taaaaagttt tgctgatgct gtggcagcgg
     4021 aaaaagcgga gctgaagcta attgcggaga aaacgaaaaa ggggtatgtg gaaggcaatt
     4081 cggcaaacgt gcatatcccg ccagtcacaa aagaaagtat cccttctgaa gttaaaactg
     4141 ctgttgtaag taaaaacaaa cacccatggc tggcagatga tgcagccatt ccggtaaccg
     4201 acgatattaa tcgatttgct tttccacatc gctctcgccc cagagagatc aattatctgc
     4261 acaaagatgg tcaaatatgg aagcgcatcg cgaataacac cagggcatat gaccccgaca
     4321 ataattaccg ctcttatcct gaaaattggc agcaagcgtt tgccgagtta caaagtcgaa
     4381 ttctggacaa tcaacaaaca gggagtgcgc aatctgatgc ggcattgctc tggagttttt
     4441 ggaactctta ctctgcagat gaactggtcg atgacttagt cattcgctgt ggtctggaaa
     4501 gtgccgttga aatcgccctt tttgctcttc aactcaaata taaaccagta aaaggcgcag
     4561 tcaccaccat cattccacct ggtcacaaag cagaatcgct accgagctgg catcaacgtc
     4621 tatgtcatca tctttcactc gcttcagaag atgagtggca acgctgtgtg aataaagtgc
     4681 ttactgctat tccgacgcta tccccagcac ggcaaccttt tgcggcgtta ctgctccctg
     4741 aacgcccgga tattgccaat gctatggctc tacgttacgc agaccaaaac gttccggcga
     4801 tgacctggtt aagactggta gtaaccgatg atgccgctct gactgcagca gaaaaatacg
     4861 acttcccacc gttatatcgt gattttcgcg cttatctggc aatgttgctg gcaaataatg
     4921 gggtgagcgg tgtaagccgg atacttactg agtttgtgga agagcattct gacaatccca
     4981 cttatctctt cgagcgcatc agcgagactg aagatcttgt caaatggtta tggaaaacga
     5041 atcacccgga tgcgattcaa attctgatcc tcggtgtaat tggcaagaaa aagcacctgg
     5101 aatacttaag caaagcctgt caaaaacatt ccgctgcggc tattgccgct tatgcaactt
     5161 tgctggcaat acatgaagat aaagagtggc ataaagcgct tgtcaaattg attaccatca
     5221 cgccagagtt agtatgcgac gtcattccct gggttaatgc taaggcggca ggcatcttat
     5281 ctgaatgtcg tccccagtca gtcgctgaag aatgtgaata cgctactgcg gatatgctac
     5341 cgcagctatt tgtcgctcca ccgtgggtaa taaataagaa gaaaaatgta atacccgtat
     5401 ttgatttacc ggtacttccc atccctgccg tcactgatat cacaccagga attacagagt
     5461 taatcagtca tactgacata tccagattct atgaaattgc gcaatatcag gcatcacagc
     5521 aaaccttatt taccgacctg ccactaattg aaaaagaaag ctgggagaca tctttcattc
     5581 cttttacacc agaacaacaa atcctttggc aattaggttt taatgaatgg ctgcattgcg
     5641 aagatgactt acatgaaaaa aaatatatac cgcaaagcgc tgttgatgcc ttgttgcgct
     5701 ttgattttcc agcactaaaa gctgagtttg cgaaatatca caacaatgcc aacaaaagct
     5761 ggaatctatc tgcgctttgt tacttaccgg gacaacaggc gatttcattt ctcaaccaaa
     5821 taatcattga ggagcaatat tcaggtgaga aagagattct ggcggttttt ggtagtacgg
     5881 ccattcctgc gtttatgacc tgcttgcaac gagatcatca acgactgtgg atattcaccc
     5941 tctttattgg ggccagtgaa ctagccttac caatggcgca gcggttacag aaaaaaatgg
     6001 cttacaagga tgccgttaat tggctggcaa ataacccgcg ccatgccgcc gccgggctac
     6061 tgcctttggc gctgggcaaa ccgtgccaaa atcgggaata tgcccgccag gcactcctcc
     6121 tgctagtgaa attaaaccag cgagaaacga ttgaggaaat cgcccaagga tataatcaac
     6181 ctgatatttt agcagcgctg gcaacattat ttgatagcga tccgcttgag gagcatcccg
     6241 caaaaatcgc cccactgccc ggcttttatc agttcacctt atggcgcaga cctcggctta
     6301 aaagtaacaa cctgcctctg ccagatgacg ctatgcgcca cctcggcaca atgttgagct
     6361 ttcctcgcga cattaccgcc tacgctgggc tggatatcat tagagaaacc ttcacccgcg
     6421 aatcactggc tgactttggc tgggatctgt ataccgcctg gacggaggct ggcgctcccg
     6481 caaaagaaaa ctgggcattt acttcgcttg ggattttagg caatgacgac accgcgcgta
     6541 aattaacccc gcttatccgt gcctggcctg gggaatccca gcataaacgg gcggtttacg
     6601 ggctggatgt gttagccagt atcggtagcg acatagcgct aatgttgctt aatggcattg
     6661 cgcaaaagat taaattcgtt gcattacagg aacatgccag cgacagaatc aatatggtcg
     6721 ccgaaaatcg cggactgaca atggctgaac tggaagaccg cctggctcca gatttagggc
     6781 ttgatatcaa cggctcgctg atactggatt tcggcccccg caaattcacc gttggttttg
     6841 acgaaaccct aaaacctgta gtgcgcgatg caaacggcaa agtcctgaaa gatttaccca
     6901 aaccaaatca gagcgatgat aaaacactgg caactgacgc cgttaacctc tttaaacaat
     6961 tgaagaaaga tgtgcgcgcc attgccagcc agcagattga tcgtctggag caggctatgt
     7021 gccagcacag acgctggacg gcagagcagt tccgcctgtt tctggtggag catccgctgg
     7081 tacgtcactt aacccgacgg ctgttatggg gggtatatac tgaggaaaac acgctgatcg
     7141 cctgcttccg agtcgcagaa gacagcactt acagcgatgc gcaggatgag ctatttacgt
     7201 tgccagcagg aaacatcggt attccgcatg tgctggaaat atctcctgaa tccgccgctg
     7261 catttgggca aatttatgct gattacgaaa agcttcctcc ctttcgccaa ctcgatcgtg
     7321 gttattacca ccttactgat aatgaacgtg atacccatga attgatacgc tggcaaggac
     7381 gtctttgcca ggccgggcgc attgtcgggc tggaacgtcg aggctggcaa cgtctggaag
     7441 aaagcggcag cgtctatgcg atgcgcaaat ctacccctta tggcgctctc gaacttgaaa
     7501 cagaacattt ttcattaatt tatggcgaaa cggggtatag cgacctgcta cctgttgaaa
     7561 gcgtaaaaat cacttcacca ggggaacgct acagtacaca gccctcgctc acgttctccg
     7621 cgcttgatgc catcaccgcc agcgaactca ttaacgacat cgaatcactt tttgattaaa
     7681 aggactttgc aatgaaaacc tatatttatc aggacgaaaa atcacataaa ttctgggcgg
     7741 tggagcagca gggaaacgaa ctgaatatca gttggggcaa agtcggcacc aacgggcaaa
     7801 gtcaggtaaa aagctttgca gatgctgcgg cggcgggaaa agcggaactc aagctgattg
     7861 cggagaaaac gaaaaagggg tatgtggaac aagcggagaa tgctccatcg aaacatactc
     7921 gaacggtaac gaaatctctc gaattatctg acttttctac catcattcag gaacagccct
     7981 cagttacacc agaaatctac gtggctgaca aaaatacaga cgttatttta ccgtggctgg
     8041 caaaagatat tgctgtcgtt tttccgcttg aagtcgtacc caccacctta agccatcgcc
     8101 gctttcccgg agatcctgtt acacctgtag ataaattaac ccagttacgt cgcttagcct
     8161 gtggcgtgtc gcaacgggat aacaaaacag ctacatttga cttcagcgcc tgtactttag
     8221 agtggcaaaa cttcatcgct caggcgatcg atctgattaa cagcccgaaa acgacaaaat
     8281 taccatcacc ggtaatggct gtactcacgg cacttgaact gcactgcacg agatataaag
     8341 agcgtgagga tgttatggat caaattattc aggaaggcgg tctggaatat gctactgatg
     8401 taataataca tcttcaacaa atatctattg aatgggatta tgtcaacaat aatattgttt
     8461 tcctgtcgtc tggcatttca cctgattact tgcagcaata ttccagcttt gaattacgac
     8521 tacgtaaaca tttatcactg gcggaagagt ctctctggca aaaatgtgca caaaaactta
     8581 ttgccgcaat tccacatatt ccagaatggc ggcaaccatt aattgctttg ttattacccg
     8641 aaaaaccagg aattgcacat gaaattgccc agcgtctact ggggcaaaag aaattaccct
     8701 cgcttgagtg gctaaaaata gtggcgactg atgagcacat tcttgcctca ttagaaaaat
     8761 atcacgagcc atatgccatt tttgatgatt actattgtgg tgcgatatgg tcagccaccg
     8821 tattacagga gcaaggtgtt gcagccctgc cccgatttgc tccctatgtc gcaagtgact
     8881 actgcgccga tgtgttgcgt catatcaatc atccgttcgc attaacactg cttatacgtg
     8941 tagccgggca tactaaacgc tgtcacgatc gaatgacgaa agcctgcgct gcgttcccac
     9001 acgcagcact ggcagcattg gcggaacttc tagtgcaaaa agaagagaat agttggcgca
     9061 ttatgctaat gacgatgctt atctcgcaac cgacactagc agaacaggtc attccctggc
     9121 tttcgacacc cgcagttgca gtgctgaaat catgccagca acaactgaca cagccctcaa
     9181 accatgccag cgccgatcta ctgccggcca tagtagtctc cccaccatgg ctttcgaaaa
     9241 agaaaaaatc gccgattcca gtgctggatt tagcaccatt aggtattgag ccaatcagtt
     9301 atctgacaga agaaatcagt aatcaacttt tggcgaaata tatctggtat tcaaaacaca
     9361 tcacggttag tcatgaagaa agtactgcca atctgttggc aaggatgggt tttcaacgac
     9421 ggatcgctgg tacatatatt aaagctcccg aagcggtagt tgaggcatgg ctaaatgaag
     9481 attattcaac cttaataagt gaatttaagg tgtttcattc acctaccggg cattattggc
     9541 atttggggat tttgacaaca ttgccgctgg agaaagcagt aagagcatgg aatgccctta
     9601 ctctatctcc gcataccgat accgaatacg ccatgttaca ttttggattc aaagggttac
     9661 ctgggttagt gaactcactt gcacgctatc cacaggaagc cttgcccata acgaattact
     9721 tcgcagcgag tgagctggca cctgccgtcg cccgcgcctt caacaaactc aaaaccctac
     9781 gacaagatgc ccgtagctgg ctgctgaaat acccgcaaca cgccattacc ggcctgctgc
     9841 ctgcagcgct cggcaaaacc ggtgaagcac aggacaacgc ccgtgctgcc ttgcgtatgc
     9901 ttactgaaaa cggtcatcag ccattgctgc aagaaatcgc ccggcgttat aaccagccgg
     9961 aagtaaccga tgcggtgaac gctctccttg cgctcgatcc cttagataat cacccgacaa
    10021 aaatccccac tcttccgacc ttttatcagc catcgctctg gacgcgcccg ttattaaaag
    10081 caaacgccca atcactgcct gatagcgcac tcctccacct cggtgaaatg ctccgcttcc
    10141 ctcaggaaga ggcactgtat ccgggattat tgcaggtgaa agatgtctgt accaccgact
    10201 cactggctga atttgcctgg gatctgttta ccgcctggca gaccgctggc gcgccgtcga
    10261 aagagagttg ggcgttcact gcgttaggcg ttctcggtaa cgatgacacc gcccgcaaac
    10321 tgacgccttt gattcgcgcc tggcctggtg aatcccagca taaacgcgcc accgtcgggt
    10381 tggatattct cgctgctatc ggtagtgata tcgccctgat gcagcttaac ggcatcaccc
    10441 agaaactgaa attcaaagca ttacaggagc gggcaaaaga aaaaatagcc aacattgccg
    10501 agagccgcga actcacggtg gcggagcttg aagatcggtt agcaccggat ctcggtctgg
    10561 atgataatgg ttcgttgttg ctggatttcg gcccacgcca gttcaccgtc agctttgatg
    10621 aaaccttaaa accgtttgtg cgtgacgctt ccggcagtcg tctgaaagac ctgcccaaac
    10681 caaacaaaag cgatgatgaa tcacaggcga acgatgctgt taaccgctac aaattgctga
    10741 aaaaagatgc gcgtaccgtc gccgcccagc aggtagcaag gctggaatcc gctatgtgcc
    10801 tgcgtcgccg ctggtcaccg gaaaactttc agctcttcct ggttgagcat ccgctggccc
    10861 gccacttaac ccgccgtctg atttggggcg tttatagcgc cgaaaaccag ctactggctt
    10921 gctttcgcgt agcggaagat aacagctaca gcaccgctga cgatgatctt ttcaccctgc
    10981 cggaaggcga tatctctatt ggtattcctc acgttctgga aatatcaccg acggatgctg
    11041 ccgccttcgg gcagcttttt gccgactacg aactgttacc accgttccgc cagcttgatc
    11101 gtaacagtta cgcgctgaca gaagccgagc gcaacgccag cgaactgaca cgctggacag
    11161 gcagaaaatg cccgagtggt cgggtaatgg ggctggcgaa taaaggctgg ataaagggca
    11221 ccccgcagga tgctggctgg atcggctgga tgatcaaccc cctgggccgc tggtcgttaa
    11281 tcatggaaat cgatgaaggt tttgcggtag gcatgtcgcc agccgaactg agtgccgagc
    11341 aactcttaag caagctgtgg ctatgggaag gcaaagcgga aagctatggc tgggggagta
    11401 attcaacaca ggaagcgcag ttctccgtac tcgatgccat caccgccagc gagctaatta
    11461 acgatattga agccctgttt gaataaggaa agagcatgga caaggaatta ccgtggctgg
    11521 cggataacgc ccaactggaa cttaaatata aaaaaggcaa aacaccactc agccatcgca
    11581 attggccagg cgaaccggtg cctgttatca ctgaaagcat cattcagaca ctgggtgatg
    11641 aattgctaca aaaagcagag aagaaaaaaa acattgtctg gcgttatgaa aacttttcac
    11701 tggagtggca gtccgccatc acgcaggcca tcaacttgat cggcgaacac aaaccctcaa
    11761 tcacggcccg gacaatggcg gcgctagcct gtatcgcgca aaatgatagc caacagttgc
    11821 tcgacgaaat cgtccaacaa gaggggctgg aatatgcaac tgaggtggtg attgcacgcc
    11881 agtttattgt acggtgttat gagagtgatc ctctggtagt aacattgcag tatcagaacg
    11941 aggactatgg ctatggttat cgctcagaaa cctataacga attcgatctc cgattacgta
    12001 agcatctctc tctggcagag gaaacctgct ggcagcgttg cgccgacaaa ctcattgccg
    12061 cactgccagg aataaccaaa gttcgccgcc cttttattgc gctgaacctc ccggaaaaac
    12121 ccgaaatcgc caacgagctg gtaagccttg aatgttcgca aactcatttt cgctcaaaag
    12181 agtggttaaa agttgttgct gatgacccta aggcggtgaa agaactcgca cgttactgga
    12241 gccaggatat atttagcgat cgagaagcca gctacatgtc gcatgaaaac cacttcggtt
    12301 acgcggcctg cgctgccctt ttgcgcgaac aaggactggc agccgttccg cgcctcgcga
    12361 tgtatgcaca taaagaagat tgcggcagtc tgctggtaca aattaaccat ccgcaagtca
    12421 tccgcacctt actactggtg gctgataaaa acaaacccag cctgcaacgt gtggctaaat
    12481 acagtaaaaa cttcccccat gcgacgctcg ccgcactggc agaactgctg gcgttaaaag
    12541 ccccccctgc gcgccctggt tatcccatca tcgaagacca aaagctgcct gcacagcaaa
    12601 aagcacgtga tgaatactgg catacgctgt tacaaacgct gatggcatcg cagccacaac
    12661 tggcagaaga ggtgatgccg tggttaagta ctcaagccca ggcagtggtg aataattatt
    12721 tatcgacatc gccaaaaccg gttattgatg gcaccgataa cagccaaatg cctgaaatcc
    12781 tcgtttcacc accgtggcgt agtaagaaaa aaatgacagc tccacgtctt gatttggcat
    12841 cgctcgaatt aactccgcaa gtttactggc aaccaggcga acgagagaga cttgccgcca
    12901 cagagtctgc ccgttatttc agcacgggat cttttacgga acgcatggaa cgaaaaagtg
    12961 gtcgagttgt actacaggaa ctgggttttg gggatgatgt atggctgttt ctgaattata
    13021 tactccccgg aaaactggat gctgcacgca attcactcat tgttcagtgg cattactacc
    13081 cagggcgggt tgaagagatc atgaatggct ggagctcccc ggaagctcaa ttagcagaac
    13141 aggcgcttcg cagcggtcac gtagaagtgt taattaacat atgggaaaat gacagctact
    13201 cacgctatcg tcgggaaaag agtatctgga acctgtattt attggcgcag ttgccgcgtg
    13261 agatggcttt gcctttctgg ctacgtatca atgagaaaaa gcatctgtct gcgggtgagg
    13321 actattttct cagtatcttc ggattggatg ctctaccagg tctgctgttg gctttttcac
    13381 atcgtccaaa agaaacattt ccgttaattt taaatttcgg cgcaacagaa ctggccctgc
    13441 ccgttgcccg cgtctggcga cgtttcgcgg cgcagcgtga tctggctcgc cagtggattt
    13501 tacaatggcc ggaacatacg gctactgcac ttatccctct tgtcttcacc aaatccagcg
    13561 ataatagcga agctgcatta cttgccctac gtttacttta cgaacaggga catggtgaat
    13621 tgctgcaaac cgcggcaaac cgctggcagc gtacagatgt atggcctgcc ctggagcatt
    13681 tacttaaaca gagtccaata gagatttacc cggcacgcat gccaaaagcc cctgatttct
    13741 ggcatccgca aatgtggtcc aggccgcgcc taatcattaa taatcaactt attaccgatg
    13801 acgctctgga aattatcggc gaaatgctgc gctttaccca ggggggacgt tttcatagcg
    13861 ggctggaaca actgaaaacg ttctgccagc cgcaaacgct ggcagctttt gcctgggatc
    13921 tcttcactgc gtggcaacaa gctggtgccc ccgcaaaaga caactgggca tttctggcgt
    13981 taagtctctt tggtgacgaa agcacggcac gggatctaac gacacagatc ctcgcctggc
    14041 cacaagaagg caaatctacc cgtgctgtca gtggcctgaa catccttacc ctgatgaata
    14101 atgatatggc gctgatacag ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac
    14161 gtgataacgc ggcggaattt cttcaggtgg tcgcagaaaa tcgcgggcta agccaggaag
    14221 agttagcgga cagattagtc ccaacactgg gccttgatga tccgcaggcg ttgagttttg
    14281 attttggtcc ccggcagttt accgttcgct tcgatgaaaa ccttaacccg gttatctttg
    14341 atcagcaaaa cgttcgccag aaaagcgttc cccggttgcg caccgatgac gatcaactga
    14401 aagcgcccga ggcactggcc cgattaaaag ggctaaaaaa agatacgact caggtgagca
    14461 aaaacctgct cccccgtctt gaagctgccc tacgtaccac ccgacgctgg tcgctggcag
    14521 attttcattc tctgtttgtt aatcatccct ttacccgtct ggttacccag cgattaatat
    14581 ggggcgtgta tccggcaaat gaaccgcgtc gtttactcaa cgcctttcgt gtggccgcag
    14641 agggggagtt ctgcaatgcg caagatgagc ccattgacct gcctgcggat gctctgattg
    14701 gcattgccca cccgttagaa atgacagaag gaatgcgcag tgaatttgca cagctttttg
    14761 ccgattacga aattatgccg ccttttcgcc agttggcgcg gcgcacggtg ctgctcacac
    14821 ctgacgagtc aaccagtaac agcctgactc gctgggaagg taaatccgct accgttgggc
    14881 agcttatggg aatgcgatac aaaggctggg agtcaggcta tgaggacgca tttgtctatg
    14941 acttaggtgc gtaccggctg gtccttaagt tttcacccgg ttttaaccac tacaatgttg
    15001 atagcaaagc gctaatgagc ttccgttctc ttcgggttta ccgtgacaat aaatccgtca
    15061 cttttgccga acttgatgtg tttgatttga gtgaggcatt aagcgcacct gacgtcattt
    15121 tccattaaaa cacagcgggc agtgatgcaa ctgcccgtta tcaacacgac ctaccagcgg
    15181 gggaagcgat gatcgtgcaa aaagagctgg ttgctattta cgattatgag atccctgtac
    15241 ctgaagagcc gttttccttc agacttgaaa tccataaatg ctctgaatta tttacaggtt
    15301 ccgtctatcg actggagcga ttccggctac atccaacatt tcatcaacgt gatcgagaag
    15361 atgccgacca ctaataaatg atgcgttgat ttatataaga gatgagttta ttgatgagcg
    15421 gaaattacga ggtgaatcac atgaaacagt aatagcaatt tttaattgtg aactacagaa
    15481 tatattcaac caagaaatag aataatatac tctagataat tcaagtcgca ggaaggcgat
    15541 aagcgagtga atccccagga gcttactaaa gtaagtgaca ggggtgaacg acaaatccgc
    15601 atggagcgga tttgaacgct acttgtagcg gccccgtaag gggtgaggtc catggatggg
    15661 ccgaataacc cgcagtcgca gcacatgcaa cttgaagtat gacaagtaaa gctttattaa
    15721 agggggaaac ggtattcccc cttaaatgcg tttcaatttg cccgttactt caacaaatca
    15781 atcaaccgta acagagccac taataactcc agtacggcaa tgagcacggt taacttttgt
    15841 gtcagcgtgt ccattcgctc tccttattaa ggagcgcaac accagcatct tacccttaca
    15901 ctgcctgtcg atactggttt acgtatgttg agtcgccatg ttgcactccg gatcagagca
    15961 ctacctgcgg caccacccgt ccggtagcca ggacttgaaa ttggttcgat ccggcgcaac
    16021 ttcccaacag tcaggattat aaatctcatt atcttaattg caacggggtc cgtaggtggt
    16081 tataattcgt tcgttggggc gccatgttgc ataatcagta acttaacgtc aaagttgtaa
    16141 ataaaagaac ctgcctcacc agcaggtttt tttatttttt gcgatctgct ttccagatat
    16201 tttccgctca aacaactaat gcgccaaaca tttattgcgc gtaaaatatc gtttatttca
    16261 ttaatacatt tcagggatga atatatgtca ccgcagaata atcacctgca gcgtccgcct
    16321 gctgctgtgt tatacgccgt tgaactggca aaattaaaac aaaatgataa cgcaccttgc
    16381 ccgccaggtt ggcagttaag tttgcctgcg gcccgtgctt ttatccttgg cgacgaagcg
    16441 caaaatatta gccgtaaagt ggtgattagt ccctctgctg tcgaacgcat gttagtcact
    16501 cttgccaccg ggcgcggttt gatgttggtg ggggaaccgg ggaccgcaaa atctctcctg
    16561 tctgaattac tggcaaccgc tattagcggc gatgccgggt taaccatcca gggcggcgca
    16621 tctaccaccg aagatcaaat caagtacggc tggaactacg ccctgcttat caatcatggt
    16681 ccgtcaacag aagcgctggt tcctgccccg ctttaccagg gaatgcgcga cggcaaaatc
    16741 gtccgttttg aagagataac gcgtacaccg ctggaagtgc aagactgtct gttaggtatg
    16801 ctttctgaca gggtgatgac ggtgccggaa ctcactggtg aagccagcca gctctatgca
    16861 cgggaaggct ttaatattat tgccactgcc aatacgcgcg atcgcggcgt caatgaaatg
    16921 agtgccgcgc taaagcgtcg cttcgatttt gaaactgtct ttccgattat ggatttcgcc
    16981 caggagctgg agcttgtcgc cagtgcttcg gcgcgtttgc tggcccatag cggcattccg
    17041 cataaagtgc ctgacgcggt actggagtta ctggtccgca cctttcgcga tctgcgtgcc
    17101 aacggcgaaa agaaaacgtc aatggataca ctaacagcga ttatgtccac cgctgaagcc
    17161 gtgaacgtgg cccacgcggt gggcgtccgc gcctggtttt tagcgaatcg agcaggtgag
    17221 ccagccgatc tggtggactg tattgccgga accatcgtca aagataacga agaagaccgc
    17281 gcgcgcctgc gccgttactt cgaacaacgt gttgccacac ataaagaagc tcactggcag
    17341 gcttattatc aagctcgcca ccgcctgccg tgaggaaaga tgcatgagcg agccgttaat
    17401 tgtcggcatc cggcatcata gcccggcctg cgcccggctg gtgaaattgt taatcgaaag
    17461 ccagcggcca cgatacgtat tgattgaagg cccggctgat tttaatgacc gggtggacga
    17521 actgttttta gcccaccagc ttccggtagc tatttacagt tattgccagt atcaggacgg
    17581 tgcggccccc gggcgtggtg cctggacgcc atttgctgaa ttttcgccgg agtggcaggc
    17641 gctacaagcc gcacgtcgta ttcaggcaca aacttacttc atcgatttgc cttgctgggc
    17701 gcagagtgaa gaagaagacg aatcacccga tacgcaagat gacagccaga ccttactgct
    17761 gcgtgccacc tgcatggata acagcgatac cctgtgggat cacttattcg aagatgaaag
    17821 ccagcaaact gcattaccct ctgcactggc gcgctatttt gcccaactgc ggggcgattc
    17881 ccccggagat gctctcaatc gtcagcgcga agcctttatg gctcgctgga ttacatgggc
    17941 gatgcagcaa aacaatggcg acgtgttagt cgtctgtggc ggctggcacg ctcctgcact
    18001 ggcaaaaatg tggcgcgaat gcccgcagga aattaacaag ccagaattgc cctctctggc
    18061 agatgccgtt actggttgtt atctcacacc ctacagtgaa aagcgccttg atgtgctggc
    18121 aggatacctt tccggaatgc ctgccccggt ctggcaaaac tggtgctggc agtgtggctt
    18181 acagcaggca ggtgaacagc tgctgaaaac ggttcttacc cgtttgcgcc agcacaaatt
    18241 gcccgcttct accgctgata tggctgctgc tcatctgcat gctatggcac tggcacagtt
    18301 gcgcggccat acactacctc tacgcactga ctggctggat gccatagctg gttcgctgat
    18361 taaagaagcc ttgaacgcgc cgttgccgtg gagctaccgc ggagtcatta atcccgatac
    18421 cgatccgatt ctggtaacgt tgatagacac attagcgggt gacggattcg gtaaacttgc
    18481 cccttctacg ccacaaccgc ctctgccaaa agatgtcacc tgcgaactgg aacgtaccgg
    18541 aatctctctt ccggcggagc ttaccttaaa tcgctttacc cccgatggac tggcgcaaag
    18601 tcaggtgtta catcggttgg caatactgga gatcccaggg gttgtgcgcc agcagggaag
    18661 tacactgaca cttgcaggca acggtgaaga atgctggaaa ttaacccgcc cgcttagcca
    18721 acatgcggca ttaattgagg ccgcctgctt tggtgccaca ctccaggaag ccgcacgcaa
    18781 taaattagaa gccgatatgc tggacgcggg tggaatcggc agtatcacca catgtcttag
    18841 ccaggcagcg ttagcgggtc tggcgtcttt cagtcagcaa ttactggaac aactcacatt
    18901 attaatcgcc caggaaaatc aattcgccga aatgggccag gcgctggaag tgctatatgc
    18961 cttatggcgg ctggatgaaa ttagcggtat gcaaggcgcg cagatattac agacgacgtt
    19021 atgcgcggct atcgatcgca cactctggct atgtgaatcc aacggcagac cggatgaaaa
    19081 ggagtttcac actcacctgc atagctggca ggcgctttgc catatcctgc gcgatctaca
    19141 tagcggcgtt aatttacccg gcgtttcgct ttctgcggca gtagccttac tggagcgccg
    19201 cagtcaggcg attcatgccc cggcgctgga tcgcggcgcg actcttggcg cactgatgcg
    19261 tctggaacat cccaacgcca gtgccgaaac ggcgctgacg atgctggcgc agttatcccc
    19321 ggcacaatcc ggcgaggcgc tgcacggttt gctggcatta gcccgtcatc aactggcctg
    19381 tcagccggta tttatcgccg gtttcagcag tcatttaaat caactgagtg atgccgattt
    19441 tatcaatgcc ctgcccgatt tacgcgcggc gatggcctgg ctaccaccac gagaacgcgg
    19501 gacgctggcg catcaggtgc ttgagcatta tcaactggtg caacttcccg tttcggcact
    19561 gcaaatgccg ttgcattgtc caccacaagc cattgcatat catcaacaac tcgaactgca
    19621 ggcactggca tcgctgcaac actggggagt tttccatgtc tgaactgaac gatcttctga
    19681 ccccccgtga gctacaacgc tggcgattaa ttcttggcga agcggcagaa acgacccttt
    19741 gtgggctgga tgacaacgcc cggcagatag accacgcgct ggagtggctg tatgggcgcg
    19801 atcctgaacg gctccagcgt ggtgaacgtt acggtggatt aggtcgttca aatctcacca
    19861 cccctgagtg gatcaacagc attcacacgc tgtttccgca acaggtgatt gagcggctgg
    19921 aaagcgatgc cgtgctgcgc tacggcattg aagatgtggt gacgaatctc gacgtgctgg
    19981 aacgtatgca gccttctgaa agcctgctac gcgccgtttt gcacaccaaa catctgatga
    20041 atcccgaagt actggctgcc gcccgccgga tagtgcacca ggttgttgaa gaaattatgg
    20101 ctcgactggc aaaggaagtt cgtcaggctt tttctggagt acgcgatcgc cgccgtcgct
    20161 catttattcc actggcgcgt aatttcgatt tcaaaagtac gctgcgtgcc aacctgcaac
    20221 actggcaccc gcaacacggc aagttgtata tcgaatcccc ccgctttaac agccgcatta
    20281 aacgccatag cgaacaatgg caactggtcc tactggttga tcaaagcgga tcgatggttg
    20341 attcggtgat ccactccgca gtgatggcgg cctgtttatg gcagttaccc ggcattcgta
    20401 cccatctggt cgcttttgac accagcgtgg ttgatctcac ggcagacgtt gccgatccgg
    20461 tagagttatt aatgaaagta caactgggcg gcgggaccaa tatcgccagt gccgtggagt
    20521 atggtcggca acttattgaa caacctgcaa aaagtgtcat tattctcgtg agcgattttt
    20581 atgaaggtgg gtcatcatca ttgctgacgc atcaggtgaa aaagtgtgtc cagagcggca
    20641 tcaaagtgct ggggctggca gcgctcgaca gcaccgcaac gccttgctat gaccgcgata
    20701 tggcccaggc gctggttaat gtcggcgcac aaatagccgc aatgacaccg ggcgagctgg
    20761 cttcctggct tgcggagaat cttcagtcat gaattcacta cgtccggaat tattagaact
    20821 gacgccgcag gccctgacgg cgttaagcaa tgccggtttt gttaagcgca gccttaagga
    20881 actggaaaat ggcaacgtcc cggaaatcag tcatgagaac ggcgctttaa tcgccacctt
    20941 cagtgacggt gtccgtaccc agctggcgaa cggccaggca ctgaaagagg ctcagtgcac
    21001 ctgcggggcc agcggcatgt gccgtcatcg cgtgatgctg gtgttaagtt atcaacgact
    21061 ttgtgccacc gcccagccta cggaaaaaaa agaagaagag tgggatccgg caatctggct
    21121 gaaagaactg gctaccctgc ccgatgccac ccgcaaacgc gcgcaggcgc tggtcgctaa
    21181 aggcatcacc attgagttgt tctgtgcgcc tggcgaaatt ccctctgccc gattaccgat
    21241 gagcgatgtg cgtttttatt cccgcagcag cattcgcttc gcacgctgtg attgcattga
    21301 aggcacactt tgcgaacatg tcgcactggc tgtgcaagcc ttcgtcgatg ccaaaactca
    21361 gcaagcggaa tttactcatt taatctggca gatgcgcagt gaacacgcca catcatctga
    21421 tgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcagc aattaagcca
    21481 ggcattatgg ctgggcggga taagtcaacc gccaatccat tacgaggccg ctttcagtcg
    21541 cgcgcagcaa gcggcggaac gctgcaactg gcgatgggtg agtgaatcgc tacggcaatt
    21601 gcgcgccagt gttgatgcct tccacgcccg tgccagccac taccatgccg gagaatgctt
    21661 acgtcagctt gcggcattaa acagtcgatt aaattgcgca caaaagatgg cgcggcgcga
    21721 cagtgttggt gaagttcctc ctgtgccctg gcgcacggtc gttggcgctg acattgccgg
    21781 agaagcaaaa cttgatcatc tgcggctggt gtctttaggt atgcgttgct ggcaggatat
    21841 tgagcagtat ggtttacgca tctggtttac cgatcccgac acaggaagta ttttgcatct
    21901 ttcgcgtagt tggcagagaa gcgaacagga aaactcaccg gcagcgacgc gtcggctgtt
    21961 tagttttcag gctggcgcac tggcaggtgg gcaaattgtt tcacaagcag caaaacgcag
    22021 tgccgatggc gagctgctgt tcgcgacccg taaccgcttg agtagcgttg ttccactgtc
    22081 gccagacgcc tggcaaatgt tgagcgcgcc gttgcgccag ccgggcattg tggctttgcg
    22141 ggaatattta cgccagcgtc cccccgcctg catacgacca ctcaaccagg tcgataactt
    22201 atttattctg ccggtcgcgg agtgtatttc gctcggttgg gatagcagcc gccagacact
    22261 ggatgcgcag gtaatcagcg gcgaagggga agataatgtg ctgacgttat cccttccggc
    22321 atctgcctgt tctccttttg ccgttgaacg catggcggcg cttttgcaac aaacagacga
    22381 ccccgtgagc ctggtttctg gctttgtcag ttttgtagag gggcaattga cactggaacc
    22441 acgggtgatg atgacaaaaa cccgcgcctg ggcgctggac gcagaaactg cgcctgtggc
    22501 accgctacct tctgccagcg ttctgcctgt gccgtctacc gctcatcagt tgctgatgcg
    22561 ctgccaggcg ttacttattc aactgctcca taacggctgg cgctatcagg aacagagtgc
    22621 tattggtcag gcagagttgc tggcgaatga cctcaccgcg gtgggttttt atcggctggc
    22681 acatgtgttg ggacaatttc gtaatacaga aagcgaggca cgggtagaag caatgaataa
    22741 cggtgttttg ctttgcgaac aattattccc catgcttcag caacaaggat gaaatagtgc
    22801 tttttattaa gagttctact cctgttccgg actgctcacg ccacagtatt aggcatatcc
    22861 tatatagccc ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga
    22921 aggctttcaa taagctgttt tcccttgttg ttgcatctgt tctggttttc tctcttgctg
    22981 gctgcggtga caaagaagaa tcaaagaaat tcagcgccaa tctgaacggc actgaaattg
    23041 ccattaccta tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc
    23101 aatttgcctc cattggtgca accaccaaag aagatgccgc caggacactt gagccgttaa
    23161 gcgctaaata caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg
    23221 cgcaggaaaa cgtgactatt gatatggaaa aagtggattt taaagccctg cagggtattt
    23281 caggaatcaa cgtttctgct gaagatgcta aaaaaggtat cactatggcg caaatggaac
    23341 tggtgatgaa agccgctggt tttaaagaag tgaaataatc tttcggcggc catgtttcgc
    23401 atggccgcca tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag
    23461 aaaataacgt aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcacgcat
    23521 catcgccgta atttccggca tcgaaacgcg gaactgctgt tcggtgagga ttgccagaat
    23581 gtcatcggtt ttaagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat
    23641 gcgacgttcc ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag
    23701 gccattgagg aatgaagaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc
    23761 tttacgtagc cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat
    23821 acgcaccagg tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt
    23881 acttagcatg aggccttcag gtgttgatga ggcaaaaagc cattttagca gtcttttaca
    23941 ggccaatcgc ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat
    24001 tacgcaaaat caactcggcc tggccgttat cttccagacg gatctcctgt aaatgcgcga
    24061 ggttaaccag atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta
    24121 atgtcagttc ggtaaagccc tctttccctt cgtgactggt cacgtagaca ccgctcatcc
    24181 gactgctgac aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac
    24241 aagggataaa tttcagcgcc tgttgatttt ccggtaacaa cgaaacatcc tgcttgctgc
    24301 gctcctgacg caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca
    24361 gataatcaaa ggcatgttct tcaaaggctt taatggcgta ttcgtcaaac gcggtgagaa
    24421 aaacaatata cgggcgatgt tccgggtcga gcatccccac catttccaga ccactgatgc
    24481 gcggcatctg gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc
    24541 cttctacggc gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata
    24601 cgcgcaggtt ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc
    24661 gtccctccat ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct
    24721 tatcccatag tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag
    24781 cccactggca ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa
    24841 atgttgcccc tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt
    24901 cccatgttta atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg
    24961 ggataattct tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc
    25021 aatttgcaga taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg
    25081 ccgctttaag tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc
    25141 gctgtcgcgg cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatggggatt
    25201 cacctgggcg tgaagcagtt tgatctctga ctgggtgagc atcgcttttt gccgctcata
    25261 ttgcccggcg aggatctgcg ccgaaagcag ttgcgcaatc ccctcgccca acgtgcggtt
    25321 gattgaactg aataaacggt ttttggcttc atacaatttg atggtgccca tcacccgctg
    25381 attttcgcca cgcaacggaa tgaccagcgt cgaccccagt ttgcattgcg gatgcaaaga
    25441 gcaacgataa ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt
    25501 taaggtataa gtcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt
    25561 aaaggccagc aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata
    25621 cagcacctgt gccactttca tgctgttcac ttcgttaaac ccctggcgca aaatgccttc
    25681 cgtcgaggcc gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat
    25741 cgcgcgttta tcgagcaata tacgcataaa caacgccgcg ccgacggtat tggtgaccat
    25801 cattggcgca gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc
    25861 caggataatc aacatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg
    25921 gttaaagact ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag
    25981 caatccttca acgatggtcg agatcatgca acttagcgcg gtcatgcccc ccatcgaata
    26041 tcgatgtaac ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc
    26101 gcccattacc gcacctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa
    26161 ccaggtgccc atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag
    26221 acgaaccgtg acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat
    26281 gactaaaaaa acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc
    26341 cgcaaaccac acttcccttt aaaacgcgta acatacattg cttgcgttta actttctttg
    26401 aactcttgca gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat
    26461 aaaatcaccc tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc
    26521 taaagttaaa ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg
    26581 aagtggcgtt gctttgtgat attaaccctg tcacgttacg cgcgtggcag aggcgttacg
    26641 gattgctgaa accgcaacgg acagacggcg gtcatcggct attcaacgat gccgatattg
    26701 accggatccg cgagatcaaa cgctggatcg acaacggcgt gcaggtcagc aaagtaaaaa
    26761 tgctgctcag taatgaaaat gttgatgtgc agaacggctg gcgcgatcag caagaaacat
    26821 tactgactta cctgcaaagc ggcaatctgc atagcctgcg aacgtggatc aaagagcgcg
    26881 gtcaggatta ccccgcccag acactcacca cacatctgtt tattcctctg cgccgacggc
    26941 ttcagtgcca acaaccgact ctccaggcgc tgctggcgat cctcgacggc gtactgatca
    27001 actacatctc tatttgcctg gcttcggcgc gcaaaaaaca gggtaaagat gcactggtgg
    27061 ttggctggaa tattcaggat accactcgtc tgtggctgga gggctggatt gccagtcaac
    27121 aaggatggcg cattgatgtc ctcgcccact cgctcaatca actacgccct gaactgttcg
    27181 aaggccgtac attgctggta tggtgcggtg ataatcgaac ctccgcccaa cagcagcaac
    27241 tcaccagttg gcaggaacaa ggccatgata ttttcccact cggcatttaa tgattcgtta
    27301 acaaatgcgc tttactgtac aatcctttcg ttaacataag gagtgcatta tgcgcatagc
    27361 taaaattggg gtcatcgccc tgttcctgtt tatggcgtta ggcggaattg gtggcgtcat
    27421 gctcgcaggt tataccttta ttttgcgtgc tggctgaacg cctgcaccag cctttcaaac
    27481 aggcggtctg cgatgatcgc cgccagtgcc accagtaacg ctccctggat cacatacgcg
    27541 gtattaaatc cgctaagtcc gatgatgatg ggcgtaccca gcgtgttggc ccctaccgtt
    27601 gaggcgatcg tcgccgtgcc aatgttgata atcaccgaag ttcgtacacc cgccagaatc
    27661 accggagctg ccagcggtag ctcgacctta cgcagtcgct gactacagct cattcccatg
    27721 cctttggcaa cttcggtcac gctggcatca atcgatccca gcccggcaag tgtcgcctgc
    27781 aggacgggca gcacaccgta aaggatcaag gcgataatcg ccggttgctg accaaagccg
    27841 atcaccggaa cggcaatcgc cagcactgca acgggcggaa aagtctgtcc aacggcggca
    27901 atagtttcca ccagtggacg aaattccgcg ccccaagggc gagtgacagc aattccggca
    27961 ccagtaccaa tgatcaccgc aaacaaactc gaaattccca ccagccagaa atgagccagt
    28021 gccagagctg caaagctttc ctgctgataa acgggtcgtg gcagttgtgg gaacaaggca
    28081 gcaaacagcg gctggctgta aggcagccag aaaatcagcg ccacaaacag agcaatcagc
    28141 cagaacagcg gatcgcgcaa catcttcata cgattacgcc tcctccagca gatcctgaaa
    28201 atgcagcgtg ccgcaaggct cgccctgcgt gttcaccacc ggcagcacct cgcatccccg
    28261 cgcgacaaac agagaaagcg catcgcgtag cgtcatcctt tcttccagtg cctcaccttc
    28321 agcccgttct tcacggcgca cgtaatctgc cacgctacgt aacgaaagca ggcgtacacc
    28381 cagctcacta cgaccaaaaa actggcggac aaaatcattc gccggacgag tcagcatcgt
    28441 cagcggatta ccctgctgca ccacttcacc gtgatccatc aatacaagat gttctgccag
    28501 ccgtagcgcc tcatcaatat catgagtgac cagcacaata gtgcgcccct gcaaacggtg
    28561 aatgcgcgtc atctcttgtt gcaacgcgcc acgcgttacc gggtccagcg cgccgaatgg
    28621 ttcatccatc agcaagactt gcggatcggc agccagcgcg cgcgccactc ccacacgttg
    28681 ctgctgacca ccggaaagct gatgcggata acgctcacgc aaatttggct ccagccccag
    28741 tagcgccatt aattcgtcga tacgatcgtc gatccgcgcc cgtgaccatt tttgtaattg
    28801 cggcacggtg gcaatatttt gcgccacgct ccagtgggga aacaggccaa tagattgaat
    28861 ggcatagccc atccggcggc gcaactccag cactggcagc gagcgaattt cttctccggc
    28921 aaagcggatc acgccactgt catgctccac caggcggtta atcattttca gagtggtgga
    28981 tttaccggag cctgatgtac caatcagcac cgaaaaactc ccctcctgaa aattgagatt
    29041 gagatcgtta acggcttttt gtgtgccgaa cagtttgctg acatggctaa attcaatcat
    29101 tacgtttcac cttcagcagt gcgataagca aatcgaacag cgcgtcggta agcaccgcca
    29161 gaacaattac cgggatcacc cccagcaaca ctaaatcaat ggcactgctt aacagcccct
    29221 ggaaaaccag cgcaccaaaa ccgcctgcgc cgattaacgc tgcaatcacc gccatgccaa
    29281 cggtttgcac catcaccacc cgcaggctgc gcagaaatac cggtaacgcc agcggcaact
    29341 gaacatgcag gaaacgctgc gccccgctca tccccatcgc tctggcgctc tccagcacat
    29401 cgcgcgggat ctggttcaag ccgactacca cgccgcgcac cagcggcagc aaggcataga
    29461 gcaccagcgc aatcagtgcg ggtgtcattc cggttcctgc tatgccgagc gttcccagcc
    29521 acggaaaggc cgtaaccagc gcggcaagcg gcgcaatcag caggccaaag agcgctaccg
    29581 aaggcacggt ctgaatgaca ttcagcagag aaaaaatcgc cccctgccgc gcggtggaaa
    29641 agtagcacca gatgcccaac ggcacaccaa tcactaacgc aggcagcacc gcaccaaaca
    29701 gcaacgtcag atgttgtgcc agcgcgtcgt caaacacatc ctgacggttg gcgtattctt
    29761 tcattaacga gagatcgtta agcgtgccgg agtacagcaa ccacagcgga acaatggcaa
    29821 tctgcatatg caacaaccag cgccacagcg gatgcgtgga gattcggcgg atggcatcgc
    29881 tacaggccag cagtgccagc gccgcagcca gccagaaacc actgccgagg ctggtacgcg
    29941 ccagcgcact gccattttgc gccagttgtg tcgccgcctt tccagctccc cacaccagca
    30001 atacgaagac gaattgcgcc agaatgagtg caaaaatgct gcctttttta gcgggaataa
    30061 aacaggctgt cagccaggcg caaccaacgc ccaccagcat ccagagagtt tgcggccaca
    30121 gctgccagag atgacgcccc tcacccgaaa ctaaacgatt aggtgcgtaa ctgataaacg
    30181 gcagcgccgc tgcgattgcc gtcagcaaca gcagcagcgc cagaacagga ttaatacgga
    30241 aataagtcac gggaaattac ttcgtccacc ctttttgtgt caggtagtcg gctgccactt
    30301 ttttggcatc cagtccttcc actgcaatgc tggcattcag ctgctgcaat gttttttcat
    30361 cgaggctggc gaagactggc tgtagccact gtgctatttg cggatactct ttcagcaccg
    30421 actcacgcac cactggcgca ggcgcgtaga taggttgcac gccttgcgga tcgcttaagg
    30481 tttgcagccc cagcgccgca accgggccgt cggtgccgta agccattgcg gcattaacgc
    30541 cagaagtttg ctgggcagcg gctttgatcg tcaccgccgt gtcaccaccg gccagtgaca
    30601 gcaactgatc ctgaccgagc ttaaagccat aggctttttc aaacgcgggt aaggcatcgg
    30661 cgcgttcgat aaactctgcc gaggctgcca gtttgaagtt gccaccctct ttcagataac
    30721 gactcagctc ggcaagcgaa gtgagtttgt ttttctctgc cacatcctga cgcacggcga
    30781 tggtccaggt gttatttgca ggcgcgggcg tcagccagat taacttgttt tgctctgcat
    30841 cgagtttttt gactttctca tagccttgct gcgcgttttt ccataccgta tcgttttcat
    30901 ctttaaagaa aaacgcgcca ttgccagtat attccggata gatatccagc tcgccagaag
    30961 tgatcgcccc gcgcactacg ggagtcgttc ccagctgcac tttatttacc gtaggaacgc
    31021 cgtgactttc cagcacctgc aaaatgatat tgccgagcag cgcgccttcc gtatcgattt
    31081 ttgaaccaac cttaacgggg gaagccgcct gtagcggcag gctcacggct gccaacaaaa
    31141 ccagtgaacc tgcccagagc tttgagagtg gcatgatgct ttcctcattc ttttactgtt
    31201 gttttcagcg aattaagaga aaagcatagt tgataatgac agggttagcc tgtctggacg
    31261 gattcagttg cagaatcaga taaatactta aagggcatat tttgcccggc gtttcttgcc
    31321 ggatgcggcg taaacgcctt atccggcata caaacatcga aaattacaac aactcaaact
    31381 cgcctttctt aacgcgtgcg gaatcagtgc cgatgaagac attgaacttg ccaggctcgg
    31441 cgtcatattt catctgttga ttccagaact tcagcgcctc aatatcgatt gggaagctga
    31501 cggtctgagt ttcgcccggt ttcagggtga ttttctcaaa gcctttcagc tgcttcaccg
    31561 ggcgactcat ggaagccgtc acatcctgca agtacatctg tactaccgtc gccccttcgc
    31621 gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt gccgtcacgc ttcatggtcg
    31681 gcgcagaaag tttcacatca gagacggtga aagtggtata gctcagacca tagccgaacg
    31741 gataaagcgc cccgttagct tcatcaaaat aacgcgaagt gtatttgttc ggcttgtcgg
    31801 cattatacgg acgcccggta ttcagatggc tgtagtacac cgggatctgc ccgacagaac
    31861 gcgggaagga catcggcagc ttgccggacg ggttataatc gccaaacaat acatcggcaa
    31921 ttgcattacc gccttcagtc cccgcaaacc aggtttccag aatcgcatca gcctgctgat
    31981 cttctttcac cagcgccagc ggacgcccgt tcatcagcac cagcaccagc ggtttaccgg
    32041 tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg aatagtgata tcggtacgac
    32101 tggaagcttc gtgcgccatc ccctgagctt caccgaccac agccaccacc acatcagatt
    32161 gtttcgcggt ttgcactgct tcatcaatca tctcttgcgg cgaacgcgga tcaactttga
    32221 ccgcctcttc atactgattc aggaaatcga taatgccttt gtcactggta acgttcgccc
    32281 ctttggcata cagcacttta ccgttttcac cgacggcgtt tttaatccct gttagcacag
    32341 taacagattg atcggcgaca cctgccgccg accagcttcc catcacgtca cgcttgctgt
    32401 ctgccagcgg gccaaccacc gcaatggttg cagatttttt cagcggcagc gtttcgagac
    32461 gatttttcag caacaccagg ctttcgcgcg ccacttcacg cgcttctttg cggtgcaggc
    32521 ggctttcggc attggtatcc accgggtcag actctttcgg ccccaaatgg ctgtatggat
    32581 cgttaaacaa tcccatatca tatttaacgt tcagtacatg gcgggcagca tcgtccagct
    32641 cttccatcgt cactttgccg gatttgatca acccaggcag atacttcgag taatactcgt
    32701 cgctcatgct catgttgatt ccggacttca gcgccacgcg caccgcatct tccgggtctg
    32761 ccgccgtgcc atgtttaatc agctctttga ttgcgccgtg atcggaaacg gtgatgcctt
    32821 taaagcccca ctggtcgcgc agaacatctt tcagcagcca ggagtcggag gtggctggcg
    32881 tgccgttcag cgagttcagc gccaccatca ccgcaccgct gcctgcatcc agccccgctt
    32941 tgtacggcgg catatagtcg ttaaacaggc gctgcggact catatcgacg gtgttgtact
    33001 ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt gacgctggtc atcaccgagt
    33061 agcgatctgc cgggctttta ccctgcatcg cttccaccat ggttttgccc attattgagg
    33121 tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc ccagcgcgga tcgcgcgaga
    33181 catcgaccat cggtgcccag gtcatattca ggccatcatc tgccgcttca taagcagaga
    33241 cacgcccgac tgttttcacc gcatcgaggt taaaagacga ggccagaccg agactaatcg
    33301 ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa gaaaagagga attttcaggc
    33361 ggcttaattc catcacctga tcctgcatgg cgcggatatc ctgacgggtt acagtgttga
    33421 aaatcgcccc aacctgaccg tctttgatca tctcgcggat cgcctctttc gggttatccg
    33481 ggccgacgct gattaagcgc agctgaccaa ttttctcatc aactgtcatt ttcttaagca
    33541 gttcggtgac gaacgcatcc cgcgcttcgg gcgttaatgg atggttgccg aataaatcat
    33601 ccgccagtgc tggctgcagg gccagactca ccgcgattcc tactgaacat agccatttca
    33661 tatggatttt tcttcctttg ttgcccgacg tggcagcgaa aatggtgcaa aaaccgtagt
    33721 ttgccataag catgatggag agagaaaaag aatgctcagt ttattgtctg aattttcaaa
    33781 atattcactc gctgaattgt catacaaggc gctattctag tttgtgatat tttttcgcca
    33841 ccacaaggag tggaaaatgt cttccatgac aacaactgat aataaagcct ttttgaatga
    33901 acttgcccgt ctggtcggtc attcacacct gctcaccgat cccgccaaaa cggcccgcta
    33961 tcgcaagggc ttccgttctg gtcagggcga cgcgctggct gtcgttttcc ctggctcact
    34021 actggaattg tggcgggtgc tgaaagcctg cgtcaccgct gacaaaatta ttctgatgca
    34081 ggctgccaat acaggcctga ccgaaggatc gacgccaaac ggtaacgatt atgatcgcga
    34141 tatcgtgatc atcagcaccc tgcgtctcga caagctgcac gttctcggca agggcgaaca
    34201 agtgctggcc tatccgggca ccacgctcta ttcactggaa aaagccctca aaccgctggg
    34261 acgcgaaccg cactcagtga ttggatcatc gtgtataggc gcatcggtca tcggcggtat
    34321 ttgtaacaac tcgggcggct cgctggtgca acgtggcccg gcgtataccg aaatgtcatt
    34381 attcgcgcgt ataaatgaag atggcaaact gacgctggtg aaccatctgg ggattgatct
    34441 gggcgaaacg ccggagcaga tccttagcaa gctggatgac gatcgcatca aagatgacga
    34501 tgtgcgccac gatggtcgtc acgcccacga ttatgactat gtccaccgcg ttcgtgatat
    34561 tgaagccaac acgcccgcac gttataacgc tgatccggat cggttatttg aatcttctgg
    34621 ttgcgccggg aagctggccg tctttgcggt acgtcttgat accttcgaag cggaaaaaaa
    34681 tcagcaggtg ttttatatcg gcaccaacca gccggaagtg ctgaccgaaa tccgccgtca
    34741 tattctggct aatttcgaaa atctgccggt tgccggggaa tatatgcacc gggatatcta
    34801 cgatattgcg gaaaaatacg gcaaagacac cttcctgatg attgataagt taggcaccga
    34861 caagatgccg ttcttcttta atctcaaggg acgcaccgat gcgatgctgg agaaagtgaa
    34921 attcttccgt ccgcatttta ccgaccgtgc aatgcaaaaa ttcggtcacc tgttccccag
    34981 ccatttaccg ccgcgcatga aaaactggcg cgataaatac gagcatcatc tgctgttaaa
    35041 aatggcgggc gatggcgtcg gtgaagccaa atcgtggcta gtggattatt tcaaacaggc
    35101 cgagggcgat ttctttgtct gtacgccgga ggaaggcagc aaagcgtttt tacaccgttt
    35161 cgccgctgcg ggcgcagcaa ttcgttatca ggctgtgcat tccgatgaag tcgaagacat
    35221 tctggcgctg gatatcgctc tgcggcgtaa cgacaccgaa tggtatgagc atttaccgcc
    35281 ggagatcgac agccagctgg tgcacaagct ctattacggc cattttatgt gctatgtctt
    35341 ccatcaggat tacatcgtga aaaaaggcgt ggatgtgcat gcgttgaaag aacagatgct
    35401 ggaactgctt cagcagcgtg gcgcgcaata ccctgccgaa cataacgtcg gtcatttgta
    35461 taaagcaccg gagacgttgc agaagtttta tcgcgagaac gatccgacca acagtatgaa
    35521 tccggggatc ggtaaaacca gtaagcggaa aaactggcag gaagtggagt aataataacg
    35581 gatggcagag tatcgccatc cgtcattcat ttaatcgttc tgtgccgtct gccccgccgc
    35641 tgccatttgg gcggcttttt gttttttata gctcaacgct gctgccggaa caggcatcac
    35701 tttaccggtt tcaatccagg tacgcaggcg gctggcatcg gcaaaatggg tatatttgcc
    35761 aaacgcgtcc atcactacca gcgccaccgg tttattattg ataaccgtgc gcatcaccag
    35821 acaatggccc gccgcattgg taaaaccggt tttggttaac tggatattcc agttatcacg
    35881 atacaccaga tggttagtat tgcggaacgg cagcgtatac gtcgggttag agaaggttgc
    35941 catatcttcc cgggtagtac ttaactgccc gatcaacgga tattgtttgc tggcaataag
    36001 tagtttggtt aagtcacggg cggttgaaac gttatgcacc gacaatccgg ttggttcaac
    36061 aaagcgcgtg ctgttcattc cgagcgattt cgctttcgca ttcatcgcct taataaaggc
    36121 tttgtaacca ccgggataat ggtgcgcaag gcttgccgcc gcgcggtttt ctgaagacat
    36181 cagcgccagc aacagcatat ctttacggct gatttcgctg ttcagtcgta cgcgcgaata
    36241 gacccctttc atctccggcg tctggctgat atccactttt agtttttcat ccagcggcag
    36301 tcgtgcatcc agcacaacca tcgcggtcat taatttgctg atagacgcaa tcggacgcac
    36361 cagatccggg tggttcgaat agatcacttt gttggtattc agatcaacaa tcatcgcgct
    36421 accggaggca atttccggtt gtgacgcggt ggtagcggct gccgttttcg caacggcctg
    36481 cggtgcaaaa ggcacagcca gcatcagggc caggctaaat aaagaaactc gaaatttcgg
    36541 catgatgagc attcagatag tggttcacgc gcacgggttg cgcaccgccg gagtaaggat
    36601 ttactgaggc tagcgacgcc atcataacga gcaaaaagtg cgatcgtcaa agaagaatcg
    36661 tgaggaaatg ctgcattgct gacatttacg ccagcaatgc agcgcaaaag aactttctag
    36721 aacaaacgat aaccgtagcc ccatagtata acggtaaggg cgagcagcac ttccagcacc
    36781 agcacgccta tcgccagtgt cgaactggag aagctaagtc cttcctcttt gttgatattc
    36841 aggaagctcg gaataccgag atacagcagg tagccggtgt aaaacagcgc caccgtgccg
    36901 accagcgcac acaaccagac cagcgggtaa agcgccacca gaccgcttaa aaacagcgga
    36961 gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg acggacgctg cggataatta
    37021 cgcgccatcc accagatgac ccgccccatc accgcaaccc cagccagcat aacgccgtaa
    37081 aacaagacag ccagcgccag tccggtaaac caggataact tcaggatagt gccatcgcca
    37141 aaattccagc caatctgtgt agtgccaatg aaggcgcaaa tcaccggaat cgccgccatc
    37201 agcaaaacgt ggtgggtgta gtgatgagaa atcgtttcgt tttcgcgatt aatcacctgc
    37261 atttcacgat cgggatggga aaacagtccc cagacatggc tcataccgcc cccttgttgt
    37321 gagttcatga acctgacagt tcaagtataa gtcagcttgt gattattttt tgttcgctca
    37381 tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg aatgattcac agggtgtatg
    37441 cttacagtca agcaaaggga gacagactgt cctatggatc tcaatacact catctcacaa
    37501 tatggttatg ccgcgctggt gatcggtagc ctggcggaag gtgaaaccgt gactttgctg
    37561 ggaggcgttg cggcacatca ggggctatta aagttcccgc tggtggtact ttctgtggcg
    37621 cttggcggca tgattggcga ccaggtgctt tatctgtgcg ggcggcggtt tggcggcaag
    37681 ctgttacgcc gtttctcgaa acatcaggat aaaattgagc gggcgcagaa acttatccaa
    37741 cgccatccgt atctgtttgt cattggtacg cgttttatgt atggctttcg ggtcattggc
    37801 ccgacgctga ttggtgccag ccagctgccg ccgaaaatct ttctgccgct gaatattctc
    37861 ggcgcatttg cctggtcgct gatttttacc actatcggtt acgctggtgg gcaggtgatt
    37921 gcgccgtggt tgcacaatct cgaccagcat ttgaagcact gggtctggtt gattctggtc
    37981 gtggttctgg tggtaggcgt gcgctggtgg ctgaaacggc gcgggaagaa aaagccggat
    38041 catcaggcgt aaaactattg ccctggataa ggtgttcgcg ccgcatccga catccggtgc
    38101 accatgcctg atgcgacgct ggcgtgtctt ttctggccta acccaatgaa ataaccacgc
    38161 acctcactgc gctattctgg gttgaactgc ggattcgcca acataaagcc gccatccact
    38221 atcaacgact gcccggtggt gtaatttgcg ccctccgaac aaagccatac caccaggctg
    38281 gcaatctcat gcgttgtgcc aaaacgtcgc aagggaatcg aaggctccgc gtcgggcttc
    38341 acgtcgccgc catccatgcc gttcattggc gtggcgatcg ccccaggtgc gactgcgttc
    38401 accaaaattt tatgcctgac caactccagc gccattgctt tggttaaccc gccgagcgca
    38461 tgtttagcgg ctgtgtaggc gctggcatcc ggcagcggcg tatgttcatg taccgatgta
    38521 atgttgatga tgcgaccgcc ctgcccttgc ttcaccatct ggcgggcagc aatttgcgag
    38581 cataaaaatg caccatcgac atcaacggta aaaatcttgc gccactcatc gaaagccata
    38641 tcaagaaacg gcgctttggt cattgcgccc gcattattca ccagcacatc aatacgcccc
    38701 agccgttgaa taagtttctc cagcgcctgc gcaccttctg gtagattgcc gagatccagt
    38761 tgcacgatct ccgcacgtac gccgtggcta accaccttac gcgcggtatc ttttgcccct
    38821 tcttcatctg agtgccaggt aatgccaata tcaaacccct gctgcgccag taataacgcg
    38881 cactctttgc cgatccccga atcggaggcg gtaattatcg cgacctgtgc catcgagttc
    38941 tccacttaac gctgaataaa tgttaagtat agaaggcgca tatcatcagc gtttgtaccc
    39001 cccgcccagc gcaccggtga gttgaatgga ggcatccagc cactgccctt gcaataacag
    39061 gccattggcc cgctcacgca gcgccgggat tctggcttcg ctgacccggg aaccagcaat
    39121 gaggcccgcg ttaaagcggg cctgcgcaag acctactaca cgcaaggcat cgcgctcaat
    39181 ttgcgcctga tgctggtttt tctccgccag tgtctgaacc tgactggctg cccgcgccac
    39241 gtcattcacc gcttcaacca ccgctttgtt gtagctggcg atagacaagt tgctttcggc
    39301 ttttgcgata tcgaggttgg cgttaagacg accactatcg aaaatgggta gcgtcaggcc
    39361 tgccgtaacg cccatttgct gcgcggaatg acggaacaga tcgcttaagt gcaacgcatc
    39421 ctgttgcaga aaggccatca ggttgatgtc aggataaaat gccgcttttg ccgcatcaat
    39481 agtgcttaac gatgactcaa cgtaccagtg cgccgcctgc aaatctgccc gccgcgccag
    39541 taaggagtac cccagttcat caggaagctg gcttgccact ttcggcaacg cgaccgtttt
    39601 aagcttcagt gactttgtct ggtgatttgt gagtgcattt aaccgtgcct caataatttt
    39661 cattttaccc gcgacatcgt tgagctgctg ccgggttttg ctggcattaa tatcggtttc
    39721 cacaccttca actgaagaag taatcccgtt ctgatatagc tggcgatcgg tcgcgataat
    39781 ggtgttctgc tctttttcga tttgctgcaa gaccgtgttt aacgccgcct gggtttgcca
    39841 ctcccagtac aggcgggcta cgctgccagc cagcaattgg cgggtttgct cgcgttccgc
    39901 cgcccgtgct ttaaccgtac ccaggcgagc agtaatctcc gcccgattct ttccccagat
    39961 atcgagatgc cagcccgccg ttaagccaaa agtaccgttg gtgtaccacg gaccggtcgt
    40021 acctgcggcc ggatcgttca aagcaaacgg ccccattaac ccttctgccg acattttttg
    40081 ccgctccata tccgcagaaa agtcgagctg cggaccatcc tgagtggcaa ttgccttcgc
    40141 ctgggcttca gccagctgaa tgcgctgttc agccacctgc atatccggtg cgctctgtag
    40201 tgcgttatta attaaggagg tgagttgatt atcgtgatac tccagccacc attggctgtc
    40261 tggccaacca tttttcagcg ccgtgggtaa tgcggtgtca acttgtgccg cgggtgtttg
    40321 ctggcttaac gcctggcggg tttcatgcat aggcgcacac ccggccagca tcagtaacag
    40381 cggaaaacag gcgatggctg gataaaaaga atcacgattc atgggggaat aatcaggtaa
    40441 gaaaaggtgc gcggagatta ccgtgtgttg cgatatattt tttagtttcg cgtggcaata
    40501 catcagtagc aataaaacga catatccaga aaaacataca ctaagtgaat gatatctttc
    40561 gatttatctt aatcgtttat ggataacggc aaagggcttc gttttttcct atacttattc
    40621 agcactcaca aataaaggaa cgccaatgaa aattatactc tgggctgtat tgattatttt
    40681 cctgattggg ctactggtgg tgactggcgt atttaagatg atattttaaa aatcagatat
    40741 ggcatataag ttgtcaggtc aggaactaga tattttgtgt acttgatttg caaacagctc
    40801 cggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa ggccggggca gcccacatgg
    40861 atgtgggctg agggcgcgtc ttacaaggac gttacctcgc gcccgacccg gtagccgaag
    40921 gggataagct gaaggtaccg cgcagcggcg attttgttcg ccagagcccg ggggtgcagg
    40981 gggaggaggc gattgtcctc cccctgcgcg ctccttgcgc cagtggcaat atgtggctta
    41041 gctcatgaaa ggagcgcaac aagatgatga agctgaataa gcgattcatt gtctcagata
    41101 agctgcgatt ccactattat cgcagcttat cgatatcaat ggcctgaata gcccttgcaa
    41161 tatcagggga attattcaac acccgaacat gctgaaataa ttccgttgct tcatcgtatt
    41221 ctttacgcaa ataactcaac cactgtttaa tccgcgcaac gtgatataac ccggtatcgc
    41281 cctgcttttc cagacgggta tatttttgca gcaaagcaac cacctccggc cacggcattc
    41341 gcggttcgtt atatttcacc acacggctca ggttgggaat attgagcgcc ccgcgaccaa
    41401 ccatcactgc gtcgcagccg ctgatcgcca tgcattgttg cgcactctgc cagtcccaga
    41461 tttcaccgtt ggcaatcacc ggaatattaa gccgctggcg aatctcgcca atcgcctgcc
    41521 agtcaatatg ttccgcgcgg taaccctgct cttttgtccg cccatgtact accagctccg
    41581 tagcaccagc ctgttgaacc gcatcggcga tttcaaattt cttctcaccg ctgtcccagc
    41641 ccagacgcac ttttacgctg acaggcaaat gcgccggtac agcttcgcgc atcgcttttg
    41701 caccctggta gatgagttca ggatctttca gtaacgttgc cccaccaccg ctaccgttaa
    41761 ccgttttcga cgggcagccg cagttaagat ccacgcccca ggaacctaac tccaccgcac
    41821 gggcggcgtt ctctgccagc cactgtggga actgccctaa caactgcacg cgaaccagcg
    41881 taccggatgg tgtccggctg gcattttgta gctcagggca aatgcgatga aagactttta
    41941 ctggcagcag ttgatccacc acgcggacaa actcggtgat gcacagatcg tagtcgttaa
    42001 cttcggtcag taattcccgc accagagagt caagtacacc ctccatcgga gccagtaaca
    42061 cacgcatatc atcacccgca aaaaaatgag gcgctatgtt agcgcctccg gtcaacggat
    42121 taaaggtctg caaagaggga ttgctgcaaa ctgcaatccc tctctatgct tgatgtatgc
    42181 ctgagttcgc tgcgcattgt gtaataccgg ggagcgaaaa aataagcgct gatttcttaa
    42241 tgtgatcggt agcacgtttt acgaattaat tgtatgatga atccatctca tctggggtgt
    42301 tgattatgag taagacactg aacattatct ggcaatattt acgcgctttc gtcctgattt
    42361 atgcctgcct gtatgcaggc attttcattg cttccctgct accggtaacc attccgggca
    42421 gcatcatcgg gatgctgatc ctgtttgtcc tgctggcctt gcaaattctt ccggcgaaat
    42481 gggtcaatcc ggggtgctac gtactgattc gctatatggc gctattgttt gtgccgattg
    42541 gcgtaggcgt catgcaatat tttgatttgc tccgcgcaca gtttggtccg gtagtggttt
    42601 cctgtgcagt cagtacgctg gtggtttttc tggtggtgag ctggagttcg caactggtgc
    42661 acggtgaacg taaagtcgta ggtcagaaag gatcagaaga atgatggcga atatctggtg
    42721 gtcattaccg ctgacgttga ttgtcttttt tgccgcccgc aaactggcgg cacggtataa
    42781 gtttcccctg cttaacccgt tgctggtagc aatggtggtg atcattcctt ttttaatgct
    42841 gactggcatc tcttacgaca gctactttaa aggtagcgaa gtgctaaacg atctgctgca
    42901 accggcggtc gtcgcactgg cctatccttt atatgagcag ctacaccaga tccgcgcgcg
    42961 ctggaaatcg atcatcacca tctgttttat cggcagcgta gttgcgatgg tgacggggac
    43021 ttctgtggca ttgttgatgg gcgcttcacc ggaaatcgcc gcgtcaatcc tgccgaaatc
    43081 agtcaccacg cctattgcaa tggcagttgg cggcagtatt ggcggtattc cggcaatcag
    43141 cgcagtttgc gtgattttcg tcggcatcct cggcgctgtg tttggccata cattgcttaa
    43201 tgcgatgcgt attcgtacca aagctgcgcg cggtctggca atggggacag cctcgcacgc
    43261 cctcggtacg gcgcgctgcg ccgagctgga ttatcaggaa ggtgcattta gttcgctggc
    43321 gctggtgtta tgcgggatta ttacttcgct gatcgcaccg ttccttttcc cgattattct
    43381 ggcagtaatg ggctaaaatt tgcgatgcgt cgcgcatttt tgatgtaagt ttcacgcgtt
    43441 gcataattaa tgagattcag atcacatata aagccataac gggttcgtaa actgttatcc
    43501 cattacatga ttatgaggca acgccatgca tccacgtttt caaaccgctt ttgcccaact
    43561 tgcggataac ttgcaatctg cactggaacc tattctggca gacaagtact tccccgcttt
    43621 gttgaccggg gagcaagtct catcgctgaa gagcgcaacg gggctggacg aagacgcgct
    43681 ggcattcgca ctacttccgc tggcggcggc ctgtgcgcgt acgccattgt cgaattttaa
    43741 tgttggcgca attgcgcgcg gtgtgagcgg aacctggtat ttcggtgcca atatggaatt
    43801 tattggtgcg acaatgcagc aaaccgttca tgccgaacaa agcgcgatca gccacgcctg
    43861 gctgagtggt gaaaaagcgc ttgcagccat caccgttaac tacacgcctt gtggtcactg
    43921 ccgtcagttt atgaatgaac tgaacagcgg tctggatctg cgtattcatc ttccgggacg
    43981 cgaggcacac gcgctgcgtg actatctacc ggatgccttt gggccgaaag atctggagat
    44041 taaaacgctg ctgatggacg agcaggatca cggctatgcg ctgactggtg atgcgctttc
    44101 tcaggcggcg attgcggcgg caaaccgttc gcacatgcct tacagtaagt cgccaagcgg
    44161 tgtcgcgctg gaatgtaaag acggtcgtat tttcagcggc agctacgctg aaaacgccgc
    44221 attcaacccg actctgccgc cgttgcaggg ggcgttaatt ctgttgaatc tcaagggtta
    44281 tgattacccg gatatccagc gcgcggttct ggcagaaaaa gccgatgcgc cgttgattca
    44341 gtgggatgcc acctccgcaa cgctgaaagc tctcggctgt cacaatatcg accgcgtgct
    44401 tctcgcttaa gcctggtgcc ggatgcggcg tgaacgcctt gtccggcttg ccagcccttt
    44461 cctggtgtcg aaaatcccgg caaacagttt gccgtttctt gcgcaaaacc agcgggtaaa
    44521 gtagcctgat ggaaattttc cttagatcga gtctcctgca tgttaaagcg cgtgttcctc
    44581 agcctgttag tcctgatcgg cttgctgctg ttgactgtgc tcggcctcga tcgctggatg
    44641 agctggaaaa ccgcgcctta tatctacgac gaattgcagg atctccccta ccgccaggtc
    44701 ggtgtggtgc tcggcacagc aaaatattat cggactggcg taattaatca gtattatcgc
    44761 taccgcattc aaggagcgat taatgcctat aacagcggta aggtaaatta tctattactg
    44821 agcggcgata acgcattgca aagttataat gagccgatga ccatgcgcaa agatttaatc
    44881 gcagccggtg tcgacccatc agatattgtt ctcgattacg caggctttcg tacgctggat
    44941 tccatcgtac gtacacgcaa agttttcgat actaatgatt tcattattat cacccaacgt
    45001 ttccactgtg agcgggcatt atttattgcg ctgcatatgg ggattcaggc tcagtgttat
    45061 gccgtaccgt caccgaaaga tatgctgtca gtacgtattc gcgaatttgc cgcccgtttc
    45121 ggtgcgctgg ctgaccttta tatttttaaa cgtgaaccgc gttttttagg gccgctggtc
    45181 cctattccgg ctatgcacca ggtgccggaa gatgcgcagg ggtatcccgc cgtcacaccc
    45241 gaacagttac ttgaattaca aaagaaacaa ggaaagtagt tatggatgta cagcagtttt
    45301 ttgtcgttgc cgtttttttc cttatcccga tattttgttt ccgcgaagca tggaaaggct
    45361 ggcgcgcagg cgcgattgat aaacgggtta aaaatgcacc ggaaccggtg tatgtctggc
    45421 gagcgaaaaa tcccggactc ttcttcgctt atatggtg
//
LOCUS       DADSZD010000017        43727 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.017, whole genome shotgun sequence.
ACCESSION   DADSZD010000017 DADSZD010000000
VERSION     DADSZD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.017"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            227..1321
                     /gene="gcvT"
                     /locus_tag="J1Z28_000857"
     CDS             227..1321
                     /gene="gcvT"
                     /locus_tag="J1Z28_000857"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBA7715713.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1345..1734
                     /gene="gcvH"
                     /locus_tag="J1Z28_000858"
     CDS             1345..1734
                     /gene="gcvH"
                     /locus_tag="J1Z28_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBA7715714.1"
                     /translation="MSNIPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1852..4725
                     /gene="gcvP"
                     /locus_tag="J1Z28_000859"
     CDS             1852..4725
                     /gene="gcvP"
                     /locus_tag="J1Z28_000859"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBA7715715.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAHLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(4891..6324)
                     /locus_tag="J1Z28_000860"
     CDS             complement(4891..6324)
                     /locus_tag="J1Z28_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA7715716.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWELRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            6369..6680
                     /locus_tag="J1Z28_000861"
     CDS             6369..6680
                     /locus_tag="J1Z28_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA7715717.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDSYFCTIEVTATSTVTLDTLTEKHAKQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            6844..7503
                     /locus_tag="J1Z28_000862"
     CDS             6844..7503
                     /locus_tag="J1Z28_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBA7715718.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(7580..8560)
                     /gene="ygfZ"
                     /locus_tag="J1Z28_000863"
     CDS             complement(7580..8560)
                     /gene="ygfZ"
                     /locus_tag="J1Z28_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBA7715719.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            8803..9069
                     /gene="sdhE"
                     /locus_tag="J1Z28_000864"
     CDS             8803..9069
                     /gene="sdhE"
                     /locus_tag="J1Z28_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBA7715720.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9050..9457
                     /locus_tag="J1Z28_000865"
     CDS             9050..9457
                     /locus_tag="J1Z28_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715721.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9497..10018)
                     /gene="fldB"
                     /locus_tag="J1Z28_000866"
     CDS             complement(9497..10018)
                     /gene="fldB"
                     /locus_tag="J1Z28_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBA7715722.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            10130..11026
                     /gene="xerD"
                     /locus_tag="J1Z28_000867"
     CDS             10130..11026
                     /gene="xerD"
                     /locus_tag="J1Z28_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBA7715723.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11051..11761
                     /gene="dsbC"
                     /locus_tag="J1Z28_000868"
     CDS             11051..11761
                     /gene="dsbC"
                     /locus_tag="J1Z28_000868"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBA7715724.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPI
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            11767..13500
                     /gene="recJ"
                     /locus_tag="J1Z28_000869"
     CDS             11767..13500
                     /gene="recJ"
                     /locus_tag="J1Z28_000869"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBA7715725.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            13591..14689
                     /gene="prfB"
                     /locus_tag="J1Z28_000870"
     CDS             join(13591..13665,13667..14689)
                     /gene="prfB"
                     /locus_tag="J1Z28_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBA7715726.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            14699..16216
                     /gene="lysS"
                     /locus_tag="J1Z28_000871"
     CDS             14699..16216
                     /gene="lysS"
                     /locus_tag="J1Z28_000871"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA7715727.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(16259..16807)
                     /gene="idi"
                     /locus_tag="J1Z28_000872"
     CDS             complement(16259..16807)
                     /gene="idi"
                     /locus_tag="J1Z28_000872"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBA7715728.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGMWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            16862..16933
                     /locus_tag="J1Z28_000873"
     CDS             16862..16933
                     /locus_tag="J1Z28_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715729.1"
                     /translation="MINQVSVYRQPPILSGCRQVKTI"
     gene            complement(16930..17055)
                     /locus_tag="J1Z28_000874"
     CDS             complement(16930..17055)
                     /locus_tag="J1Z28_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715730.1"
                     /translation="MNLLMRAIFSLLLIFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17057..18505)
                     /locus_tag="J1Z28_000875"
     CDS             complement(17057..18505)
                     /locus_tag="J1Z28_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBA7715731.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSQDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            18941..20860
                     /gene="ygfT"
                     /locus_tag="J1Z28_000876"
     CDS             18941..20860
                     /gene="ygfT"
                     /locus_tag="J1Z28_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBA7715732.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLNEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIDVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDISFNEL
                     TAEYDAVFLGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESAEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHTMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            20860..21348
                     /locus_tag="J1Z28_000877"
     CDS             20860..21348
                     /locus_tag="J1Z28_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7715733.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADIFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQANSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(21384..22751)
                     /locus_tag="J1Z28_000878"
     CDS             complement(21384..22751)
                     /locus_tag="J1Z28_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7715734.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGVLFLMILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(22787..24103)
                     /gene="guaD"
                     /locus_tag="J1Z28_000879"
     CDS             complement(22787..24103)
                     /gene="guaD"
                     /locus_tag="J1Z28_000879"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HBA7715735.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFIDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(24121..25521)
                     /gene="xanQ"
                     /locus_tag="J1Z28_000880"
     CDS             complement(24121..25521)
                     /gene="xanQ"
                     /locus_tag="J1Z28_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HBA7715736.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(25686..28556)
                     /locus_tag="J1Z28_000881"
     CDS             complement(25686..28556)
                     /locus_tag="J1Z28_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBA7715737.1"
                     /translation="MIIHFTLNGAPQELTVNPGKNVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIDAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEDIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEPVADVQLATPGVVRGKKGEVSFGEIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(28553..29332)
                     /locus_tag="J1Z28_000882"
     CDS             complement(28553..29332)
                     /locus_tag="J1Z28_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBA7715738.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWIDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIATRQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAITDHDGMRIALDGVASKALRLHDVETQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(29383..30711)
                     /gene="ssnA"
                     /locus_tag="J1Z28_000883"
     CDS             complement(29383..30711)
                     /gene="ssnA"
                     /locus_tag="J1Z28_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBA7715739.1"
                     /translation="MLILKNVTAVQLHPAKVQESVDIAIENDVIVAIDDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     SGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     STGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIGSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLGDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(30714..33812)
                     /gene="ygfK"
                     /locus_tag="J1Z28_000884"
     CDS             complement(30714..33812)
                     /gene="ygfK"
                     /locus_tag="J1Z28_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBA7715740.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDALNKLLQDEAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWELNHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATVVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISVTLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(34134..34712)
                     /gene="mocA"
                     /locus_tag="J1Z28_000885"
     CDS             complement(34134..34712)
                     /gene="mocA"
                     /locus_tag="J1Z28_000885"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBA7715741.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     EHYSVEIENAEIILDIDTPDDFITAKKRYTEI"
     gene            34816..35586
                     /gene="yqeC"
                     /locus_tag="J1Z28_000886"
     CDS             34816..35586
                     /gene="yqeC"
                     /locus_tag="J1Z28_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBA7715742.1"
                     /translation="MKSIIDPSALFIDLGAQKRPTVISIVGAGGKTSLLFWLAELFQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASFISPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDAPLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQIELLQPLQQHNVEAIWLGDIQEHPAIARRFVN"
     gene            35634..37259
                     /locus_tag="J1Z28_000887"
     CDS             35634..37259
                     /locus_tag="J1Z28_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBA7715743.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPTCSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(37300..38232)
                     /gene="arcC"
                     /locus_tag="J1Z28_000888"
     CDS             complement(37300..38232)
                     /gene="arcC"
                     /locus_tag="J1Z28_000888"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7715744.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAELLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(38280..39665)
                     /locus_tag="J1Z28_000889"
     CDS             complement(38280..39665)
                     /locus_tag="J1Z28_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HBA7715745.1"
                     /translation="MRVLIKNGIVVNADGQAKQDLLIESGIVRQLGTDISPQLPCEEI
                     DASGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRISPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(39718..40929)
                     /locus_tag="J1Z28_000890"
     CDS             complement(39718..40929)
                     /locus_tag="J1Z28_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBA7715746.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(40987..42183)
                     /gene="dpaL"
                     /locus_tag="J1Z28_000891"
     CDS             complement(40987..42183)
                     /gene="dpaL"
                     /locus_tag="J1Z28_000891"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBA7715747.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYHP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(42241..43428)
                     /gene="ygeW"
                     /locus_tag="J1Z28_000892"
     CDS             complement(42241..43428)
                     /gene="ygeW"
                     /locus_tag="J1Z28_000892"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBA7715748.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT        10608 a        10959 c        11488 g        10672 t
ORIGIN      
        1 actgatgtgt tttttggttt tttattctgt tgcgattttt gcatttttta accataagct
       61 aatgtgatga tcaattttac cttatggtta acagtctgtt tcggtggtaa gttcaggcaa
      121 aagagaacga ttgcgttggg gaccgggagt ggctccgatg ctgggtttcg tggtgataat
      181 ttcgccatgg aaaagttgtc agccccgctt attcaatgag gacaagatgg cacaacagac
      241 tcctttgtac gaacaacaca cgctttgcgg cgctcgcatg gtggatttcc acggctggat
      301 gatgccgctg cattacggtt cgcaaatcga cgaacatcat gcggtacgta ccgatgccgg
      361 aatgtttgat gtgtcacata tgaccatcgt cgatcttcgc ggcagccgca cccgggagtt
      421 tctacgttat ctgctggcga acgatgtggc gaagctcacc aaaagcggca aagcccttta
      481 ctcggggatg ttgaatgcct ctggcggtgt gatagatgat ctcatcgtct actactttac
      541 tgaagatttc ttccgcctcg ttgttaactc cgccacccgc gaaaaagacc tctcctggat
      601 tacccaacac gctgaacctt tcggcatcga aattactgtt cgtgatgacc tttccatgat
      661 cgccgtacaa gggccgaatg cgcaggcaaa agctgccaca ctgtttaatg acgcccagcg
      721 tcaggcggtg gaagggatga aaccgttctt tggcgtgcag gcgggcgatc tgtttattgc
      781 caccaccggt tacaccggtg aagcgggcta cgaaattgcg ctgcccaatg aaaaagcggc
      841 cgatttctgg cgcgcgctgg tggaagctgg cgttaagcca tgtggcctgg gcgcgcgtga
      901 cacgctgcgt ctggaagcgg gcatgaattt gtatagccag gagatggacg aaactatttc
      961 tcctttagcc gccaatatgg gctggactat cgcctgggaa ccggcagatc gtgactttat
     1021 cggtcgtgaa gctctggaag cgcagcgtga acatggcaca gaaaaactgg ttggtctggt
     1081 gatgaccgaa aaaggcgtgc tgcgtaatga actgccggta cgttttactg atgcgcaggg
     1141 caaccagcat gaaggcatta tcaccagcgg tactttctcc ccaacgctgg gttatagcat
     1201 tgcgctggcg cgcgtgccgg aaggtattgg tgaaacggcg attgtgcaaa ttcgcaaccg
     1261 tgaaatgccg gttaaagtga cgaaacctgt ttttgtgcgt aacggcaaag ccgtcgcgtg
     1321 atttactttt ttggagattg attgatgagc aacataccag cagaactgaa atacagcaaa
     1381 gaacacgaat ggttgcgtaa agaagccgac ggcacttaca ccgttggcat caccgaacac
     1441 gctcaggagc tgttaggcga tatggtgttt gttgacctac cggaagtggg cgcaacggtt
     1501 agcgcgggcg atgactgcgc ggttgccgaa tcggtaaaag cggcgtcaga catttatgcg
     1561 ccagtaagcg gtgaaatcgt ggcggtaaac gatgcactga gcgattctcc ggaactggtg
     1621 aacagcgaac cgtatgcagg cggctggatt ttcaaaatca aagccagcga tgaaagcgaa
     1681 ctggaatcac tgctggatgc aaccgcatac gaagcattgt tagaagacga gtaacggctt
     1741 tattcctctt ctgcgggaga ggatcagggt gaggaaattt acgcctcacc ctcactctct
     1801 tcgcaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
     1861 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
     1921 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
     1981 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
     2041 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
     2101 tacatcggca tgggttacac cgctgtgcag ctaccgccgg ttatcctgcg taacatgctg
     2161 gaaaatccgg gctggtatac cgcgtatact ccgtatcaac ctgaagtctc ccagggccgc
     2221 cttgaagcac tactcaactt ccagcaggta acgctggatt tgaccgggct ggatatggct
     2281 tcggcttctc ttctggatga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
     2341 gtcagtaaac tgaaaaatgc caaccgcttc tttgttgctt ctgacgttca tccgcaaacg
     2401 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
     2461 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt cggcactact
     2521 ggtgaaattc acgactacac cgcgcttatc agcgaactga aatcacgcaa aatcgtggtc
     2581 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
     2641 gatattgttt ttggttcggc gcaacgcttt ggtgtaccga tgggctatgg tggcccacac
     2701 gcggcattct ttgcggcgaa agacgaatac aaacgctcaa tgccgggccg tattatcggt
     2761 gtatcgaaag atgcagctgg caacaccgct ctgcgcatgg cgatgcagac tcgcgagcaa
     2821 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
     2881 attgccagcc tgtatgccgt ttatcacggt ccggttggcc tgaaacgtat cgctaatcgc
     2941 atccaccgtc tgaccgatat cctggcggcg ggcctgcaac aaaaaggtct gaagctgcgc
     3001 catgcgcact atttcgatac tttgtgtgtg gaagtggccg acaaagcggg cgtgctggcg
     3061 cgtgccgaag cggctgaaat caacctgcgt agcgatattc tgaacgcggt tgggatcacc
     3121 cttgatgaaa caaccacgcg cgaaaacgtg atgcagcttt tcagcgtgct gttgggcgat
     3181 aaccacgggc tggacatcga cacactggac aaagacgtgg ctcacgacag tcgctctatt
     3241 caggctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctaccac
     3301 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
     3361 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
     3421 ccgatcacct ggccggaatt tgccgaactg cacccgttct gcccgccaga acaggctgaa
     3481 ggttatcagc agatgatcgc gcacctggct gactggctgg tgaaactgac cggttacgac
     3541 gccgtttgta tgcagccgaa ctctggcgca cagggcgaat acgcgggcct gctggcgatt
     3601 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
     3661 gcgcacggca ctaacccggc ttccgcgcat atggcaggaa tgcaggtggt ggttgttgcg
     3721 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagccga acaggcgggc
     3781 gataacctct cctgtatcat ggtgacctat ccttccaccc acggcgtgta tgaagaaacg
     3841 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
     3901 aacatgaacg ctcaggttgg catcacctcg ccgggcttta ttggtgcgga cgtttcccac
     3961 cttaacctgc ataaaacttt ctgcattccg cacggtggcg gcggccctgg tatgggaccg
     4021 atcggcgtga aagcgcatct ggcaccgttt gtgccaggtc atagcgtggt gcaaatcgaa
     4081 ggcatgttaa cccgtcaggg cgcggtttct gcggcaccgt tcggtagcgc ctctatccta
     4141 ccaatcagct ggatgtatat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
     4201 gtggcaattc ttaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
     4261 tataccggtc gtgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgttgaaa
     4321 gaagaaaccg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
     4381 gcgccgacca tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
     4441 agcaaagtgg aactggatcg ctttatcgat gcgatgctgg ctatccgcgc agaaattgac
     4501 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
     4561 cagagcgaac tggtcgccga gtgggcgcat ccgtacagtc gtgaagttgc agtattcccg
     4621 gcaggtgtgg cagacaaata ctggccgacc gtgaaacgtc tggatgatgt ttacggcgac
     4681 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaatttac tgattcgact
     4741 atcttataaa ggcgcttcgg cgcctttttc gcttgttaat aaagtgcgct ttgtgtgttt
     4801 cggctgaggc gtaatataaa atattgcgag ctcaatatat taaaagagtg ccaggcctga
     4861 tgagcggagc acatcaggca catcggctaa ttacagattc tcgccgttgc tggcaatcac
     4921 ctctttgtac cagttaaagc tcttcttacg tgaacgcgac atatcgccag taccgtcgtc
     4981 atgtttattc acatagataa agccgtagcg tttgctgtac tgcccggtgg tgaacgacac
     5041 acagtcgatg cagccccacg gcgtgtagcc catcaggtcc acgccatcgt aagtcaccgc
     5101 ttttttcatc tcttcaatat gggcgcgcag gtagtcaatg cggtaatcgt cattgatgct
     5161 gccgtcatct tccactttgt cgtaagcgcc aaaaccgttt tcgacaataa acagcggctt
     5221 ctggtaacgc tcatacagct cgcaaagcgc gtaacgcaga ccaaccggat caatctgcca
     5281 gccccagtca gacgctttaa catacgggtt cggtacgctg ccttcaaaac cagagatcgc
     5341 atcgccggtg ccgccttcgg ccttcaccgc gttggtcatg taatagctga agccgagata
     5401 atcgcaggtg ccttcgcgca gtacatccag atcgccgtct tccattttga tgttaaatcc
     5461 gcggagttcc cactcgttca acacatagga cgggtaatag ccacgcaact gcacatcggt
     5521 aaagacgtaa cgttcgcgca tcgactcctg ggcaaacatc acatcgtccg ggttacagga
     5581 gtaaggatag agcggcacca tcgccagcat acaaccgact ttcatctccg ggttgatgcg
     5641 atgcgcggct ttcaccgcca gggcgctggc gacaaactgg tgatgcagca cctgatacat
     5701 cgtctcttcc gggttttcat gctcggtata caccacgccg gagcagcagt aaccgaacag
     5761 cggtgcacgc cagttacgct ggttgttaat ttcgttgaag gtcatccagt atttgacttt
     5821 atgcttatag cgttcaaata ccacttccgc gaaacggaca aagaaatcaa ccactttacg
     5881 gttggtccag ctaccatatt gctgcaccag atgcagcggc atttcaaagt gggagagggt
     5941 gatcaccggt tcgatgttgt atttcagcag ttcatcgatc atatcatcgt agaacttcag
     6001 cccttcttcg tttggctgag cttcatcgcc ttttggaaaa atgcgcgtcc aggcgatgga
     6061 agtacgaaaa catttgaagc ccatttcggc aaatagcttg atgtcctcct tgtagtgacc
     6121 ataaaaatca acggcttcat ggtttggata gtatttgcca ggcacaactt ctttggtgat
     6181 ttcgcgcggc acgccgtgtg cgccaccggt cagaacgtca caaatgctcg gccctttgcc
     6241 gcctttgttc cagccgcctt cgacctgatg agcggcaact gcgccgcccc ataagaaatc
     6301 tttcggtaag gtgagttttt tcactatcat aggctccaga tgagtcaatt cttacaggaa
     6361 atagtaccat gcagccaaac gacatcactt tttttcaacg tttccaggat gacattctgg
     6421 ctgggcgtaa aaccatcacc atccgcgacg agtctgaatc gcacttcaaa acgggtgatg
     6481 tgcttcgtgt cggacggttt gaagatgaca gttatttttg cacgattgaa gtcaccgcaa
     6541 cctcaaccgt aacgctggat acgctgacgg aaaaacatgc aaagcaggaa aatatgaccc
     6601 tgactgaact gaaaaaggtc attgccgaca tctatcccga ccagacacag ttttatgtga
     6661 ttgaatttaa atgcctttaa attgaactca cgcctgttag ttgaaatttc gcatttaatt
     6721 tcatgatttt tcggagatta atctcaaatc agaatttatt ttagctaaca ggtgttcact
     6781 ggaactattc tcagttacgc tggagaggta tacatgtgcg tgtacgtttt tccggagtaa
     6841 gttatggttc agaagcccct cattaagcag ggatattcgc tggcagagga aattgccaac
     6901 agcgtcagtc acggcattgg gctggtgttt ggtatcgttg ggctggtgtt gctactggtt
     6961 caggcggtgg atctcaatgc cagcgccacg gcgataacca gttacagcct ctatggcggc
     7021 agtatgatcc tgctgttcct cgcttcgacg ctctatcacg ccattcccca tcaacgggca
     7081 aaaatgtggc tgaagaaatt tgaccactgt gccatttatc tgttgattgc cggaacctat
     7141 acgccgtttt tgctggtggg gctggattct ccgttagcgc gcgggttgat gattgttatc
     7201 tggagcctgg cattgctggg tattctgttt aaactgacca tcgcgcaccg attcaaaatt
     7261 ttatctctgg tgacctatct ggcgatgggc tggctgtcgc tggtggtaat ttatgaaatg
     7321 gcagttaagc tcgcggcggg cagcgttacc ttactggcgg taggcggtgt ggtttattcg
     7381 ctcggggtga ttttctacgt ctgcaagcgc attccataca atcatgccat ctggcacggc
     7441 tttgtgctcg gcggtagtgt gtgccacttt ctggcgatct atttgtatat tgggcaggcg
     7501 taattggtgc gtcgttgtag gcctgaaaag acacaccagt gtcgcatcag gcgataaatg
     7561 cctgatgcga tacagagatt tactcttcca gcgaatatgg cagtggctcg atacgcaatg
     7621 tattcgcatc gtcacgcacg cggaagatgc tgtccggttc catatcgtta ttcatgacaa
     7681 cctgcaccac aacctgacca tcttccagtt tgaccgcagc cagcaccgta ccggtacgac
     7741 gccagttctc gcccattttc agctctaagt cttcacccgc ttccggcagt cggctggcgc
     7801 tacccttcag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
     7861 tctcttgtcc ggtatagcag cctttcttaa agctgatgcc gcccagcgcc tggaggttgg
     7921 tcgcctgggg gataaactgc ccgctgttgg cggcgtcaat caccggaaaa cccgcttcaa
     7981 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
     8041 tcagcatatt ggcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
     8101 accatagcaa cgtggtcgcg ccttctttga ctacctgttt ttctctcgaa ggtagttcac
     8161 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
     8221 cgtcgtctgg cgcgatggtc actttagaga acaccgcata ttttttcagt tcagtcagct
     8281 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
     8341 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
     8401 ctgtcatctg gctgacatct gctgtcacct ggccttgcat atatttttcg ctgtccgcgc
     8461 cagtaatagt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagtggc aaacgggcag
     8521 aagccgtagg ctgacgggga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
     8581 aacggcgtat taatcactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
     8641 gcgatctgtg ccttctgcga ggcgacttcc aaaaaaatga agcccttgaa ttgttgtgaa
     8701 ttaatttcgc gatcgcgatt gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
     8761 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
     8821 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
     8881 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
     8941 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
     9001 gcagaactgg aaatgatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
     9061 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
     9121 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
     9181 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggcgtatta atgctcgcca
     9241 gggggaaatt cgcctgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
     9301 cgtcaaagcg ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgacagcgg
     9361 aaaacggcaa catttatggc tggcagctga cagcatggac gaagccgaat ggcgggattt
     9421 acggcggata ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgtgc
     9481 taagccgtca gtgacatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
     9541 agctttgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
     9601 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
     9661 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
     9721 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaacaattt
     9781 taccttcgag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcctggattt
     9841 cgccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
     9901 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
     9961 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    10021 cgtaagcttc tcgattctgc tgtggctttt gcggctagtg tatcagaatc gctttagtca
    10081 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    10141 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    10201 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    10261 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    10321 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    10381 gtatctttat cgcgaaaagt ttcgtgaaga tgatcccagt gcgcatctcg cttcaccgaa
    10441 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    10501 accattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    10561 taccggactg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    10621 gggcgtggtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggtgaaga
    10681 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgt tgaatggtgt
    10741 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    10801 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    10861 tgtgttgcgt cacgcttttg ccactcactt attaaatcat ggtgcggatt tacgcgtggt
    10921 gcagatgcta ctgggccaca gcgatctctc caccacgcaa atttatacgc atgtcgctac
    10981 cgagcgtctg cgacaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    11041 aggaaaattt atgaagaaag gttttatgtt atttaccttg ttggcagcgt tttcaggctt
    11101 tgttcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    11161 cgatattcag cccgcgccca tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    11221 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    11281 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    11341 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    11401 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    11461 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    11521 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    11581 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    11641 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    11701 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    11761 attcgcgtga aacaacagat acaacttcgt cgccgagaag tcgatgaaac ggccgacttg
    11821 cccgctgaat tgcctccctt gctgcgccgt ttatatgcca gccggggtgt gcgtagcgcg
    11881 caggaactgg aacgcagtgt taaaggcatg ctgccctggc agcaactgag cggcgtcgaa
    11941 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    12001 gatttcgacg ccgacggtgc caccagcacg gctctaagcg tgctggcgat gcgctcgctt
    12061 ggttgcagca atatcgacta cctggttcca aaccgtttcg aagacggtta cggcttaagc
    12121 ccggaagtag tcgatcaggc tcatgcccgt ggcgcgcagt taattgtcac ggtggataac
    12181 ggtatttcct cccatgcggg ggttgaacac gctcgttcgt tgggcattcc ggttattgtt
    12241 accgatcacc atttgccagg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    12301 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    12361 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    12421 attcctaatc tggcagaact gctggatctg gtagcgttgg ggacagtggc ggacgtcgtg
    12481 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    12541 aagtgccgtc cggggattaa agcgctgctg gaagtggcca atcgcgatcc acaaaaactc
    12601 gccgccagcg atttaggttt tgcgctgggg ccacgtctca atgctgccgg gcgactggac
    12661 gatatgtccg ttggcgtggc gctgttgttg tgtgacaaca ttggcgaagc gcgtgtgctg
    12721 gcgaatgaac tcgatgcact aaaccaaaca cgtaaagaga tcgaacaggg aatgcaggtt
    12781 gaagcgctca ctctgtgcga gaagctggaa cgtagccgcg acacgctgcc aggtgggctg
    12841 gcaatgtatc accctgaatg gcatcagggc gttgttggca ttctggcatc gcgtatcaaa
    12901 gagcgttttc accgtccggt tatcgccttt gcgcctgcgg gcgacggtac gctgaaaggt
    12961 tccggtcgct ccattcaggg gctgcatatg cgcgatgcgc tggagcggct agacacgctt
    13021 taccccggca tgatgctgaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    13081 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggac
    13141 ccttcgctat tgcaaggcga agtggtgtcg gacggtccgt taagtccggc cgaaatgacc
    13201 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    13261 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgagcg tcatttgaaa
    13321 gtcatggtcg agccggtcgg cggcggtccg ctgctggatg gtattgcttt taatgtcgat
    13381 accgccctct ggccggataa cggcgtgcgc gaagtgcaac tggcttacaa gctcgatatc
    13441 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    13501 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    13561 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    13621 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact atgacgccaa
    13681 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    13741 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    13801 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    13861 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    13921 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgctacct
    13981 cgatattcag gcggggtctg gcggtacgga agcacaggat tgggcgagca tgcttgagcg
    14041 tatgtatctg cgctgggctg aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    14101 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttatgg
    14161 ctggctgcgt acagaaaccg gcgttcaccg cctggtgcgt aagagcccgt ttgactccgg
    14221 cggtcgtcgc cacacgtcgt ttagttcagc gtttgtttac ccggaagttg atgatgatat
    14281 cgatatcgaa atcaatccgg cagatctgcg catcgacgtt tatcgcgcgt ccggcgcggg
    14341 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt actcacatcc caaccgggat
    14401 cgtgacccaa tgccagaacg accgttccca gcacaagaac aaagaccagg ccatgaagca
    14461 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    14521 ggaagataac aaatccgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    14581 ctcccgcatt aaagatctgc gtaccggggt agaaacccgc aacacgcagg ccgtgctgga
    14641 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    14701 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    14761 gcgtcgtgag aagctggcga acctgcgtga gcaggggatt gccttcccga acgatttccg
    14821 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    14881 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgtc gtattatggg
    14941 taaagcgtct ttcgttaccc tgcaggacgt tggcggtcgc attcagttgt acgttgcccg
    15001 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgatat
    15061 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    15121 cgagctgcgt ctgctgacca aagcactgcg tccgttgccg gataaattcc acggcttgca
    15181 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    15241 caacaccttt aaagtgcgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    15301 cggctttatg gaagttgaaa cgccgatgat gcaggtgatc cctggcggtg ccgctgcgcg
    15361 tccgtttatc actcaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    15421 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    15481 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    15541 tatggcttac gcagattaca aagatcttat cgagctgacc gaatcgctgt tccgtactct
    15601 ggcacaggat attctcggta agacggaagt gacctacggc gacgtaacgc tggacttcgg
    15661 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaactga
    15721 catggcggat ctggacaact tcgactctgc gaaagcgatt gctgaatcta tcggcatcca
    15781 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    15841 agcacatctg atccagccga ccttcattac tgaatatccg gcagaagttt ctcctctggc
    15901 gcgtcgtaac gacgttaacc cggaaatcac agaccgcttt gagttcttca ttggtgggcg
    15961 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc agcgcttcct
    16021 ggatcaggtt gccgcgaaag atgcaggtga cgacgaagcg atgttctacg acgaagatta
    16081 tgtcaccgca ctggaacatg gcttaccgcc gacagcaggt ctgggaattg gtatcgaccg
    16141 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    16201 gcgtccggta aaataagcat tacgttatgc tcacaacccc ggcaaccgtc ggggtttttt
    16261 atttaagctg ggtaaatgca gataatcgtt ttctggcttc gcgatttgtc gcctgcatca
    16321 ccatccacgg actgaacgcc cacggcgtag catcaatacc gcgtaaaacc gctgctaaat
    16381 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtcgtgcgtg
    16441 cggcaaatac cggacacact tcattttcca caatgccatt cggatcggtg gcgcggtagc
    16501 gaaagtcagg atagatagat tcaggaggcg taatttctac gccaagctca taacggcaac
    16561 ggcggatcac cgcctcttcg ttgctttctc ccagttgtgg gtgcccacaa accgagttag
    16621 tccacatgcc aggccatgct tttttgctaa gggcgcggcg ggtaactaat aattgccctt
    16681 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    16741 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    16801 tttgcataat atctcacatg taattctgat cactcatcat tatagataat agaagagtga
    16861 aatgattaac caagtgagcg tttatcgaca accgcccatt ttgagcggat gtcgacaggt
    16921 aaaaaccatt taaaaaagca gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    16981 acaatcagaa atgacaggaa tagagagagt aaaaatcaac agcagactaa atatagcgcg
    17041 cattaaaaga ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    17101 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    17161 catgatgctg gtagccatta aagaaaacgt tcagcacaac ggcgctaagg gttgcgagca
    17221 taatgccgct atgcagcaac ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    17281 agagcgttgg cgtcatgcca acaccgagac tgatcgccac aatatagagg ttgtaacggt
    17341 tagtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    17401 tcaccagccc cgcgccgccc agcacaaatt gcggaatcga ggccaccagc accgccattt
    17461 tgggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctgt
    17521 ggacgcgcgt cacgctgacc agcccgacgt tctgcgaaaa tgacgtatgt gggaagctgt
    17581 taaacgtgcc tccaatcatt gtcccgaccc catctacgcg taacccacga ataatgtcct
    17641 gggaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    17701 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    17761 taccaaatga catcggcgtg acaatcgcaa accatgaagc atcatgtagc ccggataaat
    17821 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    17881 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    17941 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccggcggccc
    18001 agtcaatacc cacctgaatg atgctaagcc cgatagaagt aataaccaca ccggtaacca
    18061 gtggcgggaa taaaggcatc aagcgaccga taagtggcgc taataatgtg gtgataaaac
    18121 ccgcggcgat agtggcaccg aatatcccca gcaggccgat atccgggttc ataccaatgg
    18181 ctatcattgg tgttacagcg gcgaaggtca ccgacataat caccggcagg cggatcccca
    18241 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    18301 agctaatgag catcgcaata gcttcttttg ataggccgag tcggtcacca atcataagag
    18361 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    18421 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    18481 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag agtaaaaagc
    18541 aaccgtttat gttcatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    18601 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtggtt aaaccacgat
    18661 tatgagaaga gaacctgaca atggaattat ctgtaatttt ctcatattga tatttcatta
    18721 atcattctgg catgtcgcac tctcgcattt aatcgttttt atctggatag cattcttttg
    18781 atcggcaatg ttgaaataat gaaagcggca tagattgagg ggcaataatt tgaactcagt
    18841 tgcaaaatta taattaaaac ggtcatgcta cctaatatat gcccgtttat tgcatcgcga
    18901 aatttgagaa gacataactc attaaatgaa agggatgcaa atgaataagt ttatcgctgc
    18961 tgaagctgcg gaatgtatag gctgccatgc ttgtgaaatt gcctgtgcgg tggcacataa
    19021 tcaagaaaac tggccgctga gtcacagtga ctttcgaccg cgtatccacg ttgtagggaa
    19081 aggccaggct gcgaatcccg tggcctgcca tcactgcaac aatgcccctt gcgttacggc
    19141 ttgtccggtt aatgctctga ctttccagtc cgatagcgta caactgaacg aacaaaaatg
    19201 tattggttgt aaaagatgcg caatcgcttg cccctttggc gtcgttgaga tggtcgatac
    19261 gattgcgcag aaatgcgacc tttgtaacca gcgcagttcc ggcacgcaag cctgtattga
    19321 cgtctgccca acacaggcgt tacggctgat ggacgataaa gggttgcagc agataaaggt
    19381 ggcccgccag cgcaaaacgg cagcaggaaa agcgtcatca gacgctcagc catctcgcag
    19441 tgcagcgttg ctccccgtta actcgcgtaa aggcgcagat aaaatttcag cgagtgaacg
    19501 gaaaacccac tttggcgaaa tctattgcgg gctggatcca caacaagcga cttatgagag
    19561 tgaccgctgt gtttattgtg ccgaaaaagc taactgcaac tggcattgtc cgctgcataa
    19621 cgctattccg gattacatcc gtctggtaca ggaaggaaag attattgaag cggcagaact
    19681 ttgccaccag accagttcct tacccgaaat ctgcggcagg gtatgtccac aggaccgtct
    19741 ttgtgaaggc gcatgtactt tgaaagatca ctctggcgca gtctctatcg gtaatctgga
    19801 acgctacatc accgatactg cgctggcgat gggctggcgt cccgatgtca gcaaagttgt
    19861 tccccgtagc gaaaaagtgg cggtgattgg cgctggacct gcaggattag ggtgtgctga
    19921 tattctggcg cgcgcgggag ttcaggttga tgtctttgat cgccatccag aaattggcgg
    19981 tatgctgact tttggcattc ctcctttcaa actcgataaa acggtattaa gccagcggcg
    20041 agagatattc accgcaatgg gaatcgattt ccatctcaac tgtgaaattg gccgcgatat
    20101 ctctttcaac gaattaacgg cggaatatga tgctgttttc ctcggcgtgg ggacttacgg
    20161 gatgatgcga gcagatctgc cgcatgaaga tgcgcctggt gtcattcagg cgttaccgtt
    20221 cctgaccgcc catacccgcc agctcatggg attgccggag tctgcagagt atccgctgac
    20281 ggatgtggaa ggtaagcgag ttgtggtatt gggcggtggc gatacgacaa tggattgttt
    20341 gcggacttcc atccgcctta atgctgccag cgtgacctgt gcgtatcgtc gtgatgaagt
    20401 cagtatgccg ggttcgcgca aagaggtggt caatgcccgc gaggaaggtg ttgagtttca
    20461 gttcaatgtt cagccgcaat atatcgcttg tgatgaagat ggacgcttaa cggcggtggg
    20521 cctgattcgt accgctatgg gtgagccggg gccggatggc cgccgtcgtc ctcgtccggt
    20581 tgcgggttca gagtttgaat tgcccgccga tgttctcatt atggcctttg gtttccaggc
    20641 acataccatg ccgtggttgc agggcagcgg aattaaactc gataaatggg gcctgattca
    20701 aaccggcgac gtcgggtatt tacccaccca gacgcatctg aaaaaagtct ttgctggtgg
    20761 tgatgcagtt catggcgcag atctggttgt cactgcaatg gccgcaggaa ggcaggcggc
    20821 gcgcgatatg ttaactctgt ttgatacgaa ggcatcgtga tgaaatcgtt aattatcgtt
    20881 aatccggctg actgtattgg ctgccgtact tgtgaagtgg cctgtgtggt cgctcaccct
    20941 tcagaacagg agttgaatgc cgatatcttt ttaccccggc tgaaggtaca acggctggat
    21001 agcatcagtg cgccggtgat gtgccatcag tgtgaaaacg ccccttgtgt tggcgcttgt
    21061 cccgtggggg cgctgacgat gggcgagcag gtggtgcagg caaattctgc ccgttgtatt
    21121 ggctgtcaga gttgcgtcag tgcgtgcccg tttggaatga tcaccattca gtcattgccg
    21181 ggggataccc ggcaacaaat cgtgaaatgc gatctctgtg aacagcggga agaggggcca
    21241 gcctgcgttg aatcttgccc gacacaggcg ctgcagttgc tgactgaaag agaactcagg
    21301 cgagtccgcc agcagcgtat tgttgccagc ggtgagaatc ctctctgaaa gaaatctccg
    21361 cgcggaaagg cgcggagaat ctattagatt gcccaaccac ccgcgtaaaa cgcgaccagt
    21421 gcggcagtaa tgatcaccgt accaatattc agcttttgcc attcgcgcgc aaagacgcga
    21481 cccaccacca gtgttacaaa gcccagcata ataccggtaa cgatattaca agtcagaacg
    21541 atgaacacgg cacacaccag gccagccatt gcgtcaataa aatcgttgaa atccagcttc
    21601 gagacgttgc ttaacatcag caaacccacg tacatcagtg cgggagctgt ggcataaccg
    21661 gggatcagaa acgataacgg tgataaaaac agaatcatca ggaacaacac accaaccacg
    21721 gttgcggtta aaccggtttt ccccccggcg gcggttcctg ccgctgattc gatataaacc
    21781 gccgcgggcg ctgcgcccac caggccggag aatattgaac ttactgagtc actggtcagg
    21841 gctttgccgc cgttgatgat ctggttgtct ttatccaaca aattcgcctg accggcgacg
    21901 gcacggatgg tgccagtggc gtcgaacact gcggtcatca ccaatgccag tacactcgga
    21961 agtacagttg gctggagtgc gcccataata tcgaggctga aaatcagaga cttaccatct
    22021 tcgccagtca ggcttggcat cgccaccaga ccgtggtact tcaccgctgg gtcaaagatt
    22081 aagccgatga tcgaaattgc aataatcacc aacaagatcc cgccgggtac gcggcacttt
    22141 tccagaccga agatgaccgc cagccccagt aagctcatca tcaccgggaa ggaggtaaag
    22201 gcaccgagcg ccactggcaa gccttcaatc gggtttttta taaccattcc cacaccgtta
    22261 gcagcaatca gcagcagaaa caggccgata ccgatacctg taccgtgagc gatccccatc
    22321 ggcaaattac gtaagatcca ggtacgcaca ccggttacgg aaatagcggt gaagatgacg
    22381 cccatcagaa agactgcgcc cagtgcgaca ggaacgctaa tttgttgccc gagtaccaga
    22441 ctgaatgcgg taaacgccgt caaggaaata gcgcaaccaa tcgccattgg caaattggcc
    22501 cataacccca tcagcaacga gccgaagccc gcgaccagac aggtggcaac aaacacagct
    22561 gcgggaggaa aacctgcttt gcccagcatt cccggaacga cgataacgga ataaaccatg
    22621 gccagaaagg tcgttaagcc agccagtact tcctgacgaa cggtactgcc acgagcggta
    22681 attttaaaat aattatccag cgcgccctgt ggctttggtg cgtccggtgt ttgtaggatg
    22741 tctccagaca taatgatgtc ctcacgagga tgttaaagtt ttattattag ttgcgttcgt
    22801 acaccagacg accatcaacg taggtgcggt agatcgaacg gtcatcgccc aacgtcatca
    22861 tcacgaacaa tttgtcgact aaagaaacag agttgtcata gcgcagctgc tgtagcggag
    22921 tggcagtggg ttccatcacc acgaaatcag cctctttgcc aggtaaaaag ttgccaatca
    22981 aatcgtcaag gcccagagat ttcgctccgc cgagcgtggc caggtaaaac gcttcatatg
    23041 ccgagaggcg atagccttgt aattgcaata ccttgtaggc ttcgttcagc gtttgcagca
    23101 tgttaaaagt tgttccggca ccgatatccg ttcccatgcc cactttgact ttcttctgcc
    23161 atgctttttt caagttgaat aagccgctgc cgaggtaaag gttggaggtc ggacagaaag
    23221 caatgctgga tttggtttcg ctgagacgat cccactcttt ttcttcgaga tggacgcagt
    23281 gagcaaagac gcagttttta ccggtcaggc cgtactgatg gtaaacatca agataaccat
    23341 catggtcagg ataaagcgat ttcacccagg caatttcatc tttgttttca cagagatggg
    23401 tatgtaccca tgtatccgga tattcttctt tcaggcgttg cgccatcgcc atctgttcag
    23461 gagatgaggt aggggcgaag cgtggcgtaa tcgcatacaa cagacgacca tttttgtgcc
    23521 agcgttcaat cagttctttg ctttggtgat agctgctttc ggcagtgtcg agcagataat
    23581 ccggtgcgtt tcggtccatc atcactttac cggcaatcat acgcatattg atatgactgg
    23641 cggcttcaaa cagcgcatca acggattgtg gatgaacagt gccaaacacc agcgccgtgg
    23701 tggttccgtt acgtaaaagc tgcttgatga agaacgccga catctcgcgg gcgtactcta
    23761 aatcctcata acgacgttca gtagggaagg tgtgtttatt caaccactcc agcaattgtt
    23821 caccataggc ccccaccatt tcactttgcg gataatggat atgtgtatcg ataaagcccg
    23881 gtactatcag tttgccgcga tagtcgcgca cgcgaatggt gtcaggaatt tgatgctttc
    23941 cgtcttccca ttcgccaaac cattccactt ttccctgttt aatgagtaat aaaccatcct
    24001 caataaaccg cagcgcagag gcaatttctt ccgggttatc gaccgtacgg gtgacatcaa
    24061 taaaactgcc tcgtaccgct tttaacgtgt gttctcctga catcattgac tcctttactg
    24121 ttaatccatc tcttccgctg aggtaatacc aggcagaacg ttttcctgtc ggtagccacc
    24181 agggaggata atatttaata aaatcgcagt taacccgcca gcacaaatag ggttttcaac
    24241 taatacataa atagaggctg gcaatatttt aaaaatttcg ggatcgtagg agacgccaag
    24301 tcctaaacct aaagaagtgg cgacaataag tgtttctcgg cgctttaaac cgttggtgat
    24361 gatgatgcga atccctgcga tggcaatcat ggaaaacatc aacgtcattg cgcctcccag
    24421 aactgccgag ggaatggtcg taaaaaagcc gccaatcatc ggaaataagc cgaggataac
    24481 cagcattacc gcgatggttc gcccgacata acgtgaagcg acgccagtca tctgaataac
    24541 cccattattt tgcgcaaacg tggttaatgg taatgaaccg acagcggagg cgataacaga
    24601 aaccagacca tctgccagca cgccgccttt cagtcgggac tgatactctt ccccctgaat
    24661 gggacggcgg gaaaccattg ccgtggcggt gatatcgccg acagcttcca gcacgctaag
    24721 cagataaatc gtgccaacca ccaggaactg atggaagcta aaactaaagc cgtatttgaa
    24781 cggatgcggg atggtgatta acggcaaatt gcgcatactg ctgaaatcca ccatgcccag
    24841 gcataacgat gcgatatagc cgacacatag cccaatggcg atcccaccca tgcgtagcaa
    24901 cggactgcgg cagcagttaa agccgatcac cacaatcaaa accaataagc caacgccgag
    24961 atgttcgtaa ttgccgaacg taccgctgct tttggctgca aatcctccac caaaatcaat
    25021 aatgccgact ttaatcaggc ttaagccgat catcagcacc acaatgccgc tgacggtagg
    25081 cgtaataacc cgacgtaaat agggcaggat aaaagaagag ccgacaacca gaaatgcgcc
    25141 aacgaaggag acgccgagga gcgacgacat gattaactct tcgtgaaaac cgtcgctttt
    25201 catgctgctg cccagcgcaa tcatgaccgt aacaaatgaa aaattgactg actgaattga
    25261 aagtaaacca gaaccgacga tgccgtagcg gtttacttgt aaccaggtac caataccaga
    25321 ggcgatcatc gccatagaaa caagataggc agttgtttca gcggaaagct gtaaggccgc
    25381 acccacgatt aacgcggggg ttaccatcgg aacgaaaatt gccaacagat gggtaatggc
    25441 acctacgaga gcctgatgaa agggagggcg atcctccagt tcaaatataa ggtcagaacc
    25501 tgcatggttt atatcagaca tcccttcact ccttttaaag taaatttgat catctgaatt
    25561 agtaaaataa attcccacga atttggggcg caggacgcct tgagaacggc ttatctcatt
    25621 ttcgcagtca ctatttagtc atcgttcaaa cagagaaaca ggccgccgaa gcggccattt
    25681 attacttata ttttttccag cgcggtgagt attttctccg gtgtgaaatg ccattcgcgt
    25741 aaccagatgc cgcaggcatc gtgaattgcg gtagcaatcg ccggagcggc gccatttaca
    25801 ccgatttccg agatcgattt cgccccgaat gggccgactt tatcgtcgct cggcaccagc
    25861 acagcgcgga aatcacgcgg aatgtcacca attttcggtg cgccgtaact gcgtaaatca
    25921 cgcgttaacg ggtgaccttc ggcgtcataa atgatctctt cgctcatact gtggccgata
    25981 gcacgcaggg tggcaccgta gatttgtccc agcgctaact ctggattgac cggtgtaccg
    26041 cagtccagca aggcgtagaa tttatccagg cggatttcac ccgtacgcgt gttgacggca
    26101 acttcagcga agtttgcgcc atacgggaag gcgaaatcag gcgtgatata actgccagtt
    26161 cccaccagtg aaccaaagcc ggtgccggtt tcgcctttat gggctatctc tccgaaacta
    26221 acttcgcctt tcttgccgcg aacgacgccc ggcgttgcta gttgaacatc tgccactggc
    26281 tcacccaaca tttgcgcgcc gtggaacagg attttctccc gcagattttc cgctgccaaa
    26341 cgcgcagcgt tacccgagaa gcaagtaccg gacgaggcat atgcgccttt atcaaacaac
    26401 gcatgatcgg tatcaccgga gataacatgc acgtcctgcg gggggcagtg cagcacttct
    26461 gctgccagtt tcgtcaccac agtatccagg ccagtaccaa tatccgcacc gccagaatga
    26521 acgataaagg taccgtctga ttccagtttg atcatgcagt tagcctgatc gatatctggg
    26581 atccctgatt tctgcatgat aatggcgacg ccgcgaccga tgtgccagtc accgttttgt
    26641 ggttttggtg aagaccattg gatcatctcg cgaccctgac gcaggatctc ttccagtgcg
    26701 cagctggcgg cggaaggaac ggaggtcggc gctttacctt caccgattgc accgagaatt
    26761 ttcagctctt gcccttcgtg tacccggtta cgttcgataa tttccagttg gtcgatctgt
    26821 aactgttcag ccagttccgc taatgccatg gtgatagcga agttaccttt cggtgcgcca
    26881 taaccctgat aagcaccatt tgggcaaatg ttgctgtagt aggtggtgac ctggaaatcg
    26941 acgttatcgc atggatataa cggcagcgac agcgccggtc cgttacacgg tacggtgagt
    27001 gagtggttac cgtaagggcc agtgttggcg cggaaatcca tcttcactgc cgtcaggcga
    27061 ccatcttttt tcgcgcccag tttgacggtg actttcgcta cgtgacgaga ggtgttagca
    27121 ataaactctt cttcacgggt gtagcggaaa agtaccggac gcccggtcac gcaagttgcc
    27181 caggcgcaca cttcttccag caggatgtcc tgtttagagc caaaaccgcc gcctacacgt
    27241 tctttaatga catgaacttt atgttgtttc atgcccacga ggcgcgcgac ctggcggcgt
    27301 aagtgccatg gtacctgggt ggaggcgtgg atcaccagac gatcgccgtc catacgggta
    27361 aagcagatat gtgtttcagt cgggcactgc tgcgcctgcg ttgagttata ggttcgctca
    27421 atgatcacat cggcatcggc aaagcctttg tccatatcgc caatatgacc atgaatactg
    27481 gcagcgatat ttttgcgtgg gcgagaaccg atcgggaagt tgatgatcat atgctcgccg
    27541 cgctgggctg catggctgtt atcatcttct agagtatctg gcgcaccagc aacatacacc
    27601 accggttcat cgtgcacgac aggcgcatct tcagccattg cctcgtcaat cgacattacc
    27661 ggcttaagca cttcatattc aacgtcgatg agcttcagtg cttcgagcgc aatgtcttca
    27721 ctttcggcta ctaccgcagc aacgcgatcg ccgacgtgac gcattttctt gccgaacatt
    27781 cggcggtcaa gcggtgacgg ttccggtgcg ctctgaccac ccggggtata gtaaatatcc
    27841 gggcaattca ggtgagtaat aacgtgaacg acgcccggta aggcttcagc tttgctgaca
    27901 tccagatgag taatcagtgc gtgagcgtgt gggctacgta acattttaat gacgcaggcg
    27961 tcagccgtta cgcgatcttc aacatagcag ggtttcgcct gcaccatttt cgcggcatca
    28021 gttttaggat aatgcttgcc aatgacttca aggtcgtcac ggaaagtcgg agcgatatca
    28081 atggtggcct gcggattatt tttacgtgca accgccagtt caatgacctg atagtattgc
    28141 tgccagccag catcgcggct aaacagacct gagagtgcgt cgtcgatctc ttcgcgggta
    28201 ggtgcgtcga tgcgatcgag aagatcggtg ataatcagag ccgcagctgg atcgttataa
    28261 ccagactgca ccacgccaac atcaaccatg gcctgttgaa ccagacttaa ctcattccat
    28321 ttgcccagag attctgcggt acgaatatct gccttctcta actgtgcggc gataagcaag
    28381 gacgcgttaa cgatattacc gttaaagata attgcgtcag aaccggcaaa cccaaaacca
    28441 tcatcactgt tgcgtacaga gtgcattccc atgttaaaca acagcttttg cacgtttttg
    28501 cctggattaa cggttagctc ctgaggcgcg ccatttaaag taaagtggat gatcatacgg
    28561 cttcctcccc agcctgttgg cagtcggcat acagatcggc taccagaact cccgtgatat
    28621 agcgtttata ggccacgctg ccccgcaaat cttcctgcgg gaaaatggcg ttggcgacag
    28681 cctgttcaag tgcattgcct tccagatttt gagtttcaac atcatgcaga cgcagtgctt
    28741 tactggcgac gccatccagc gcaattcgca ttccgtcgtg gtctgttatt gcaacggctg
    28801 ccgtcacgac ggttaaacct gcctgagaac ggctaatttt gcgagtcgca caggtgcgat
    28861 acggatcttt aatgataatt tcggttaaca ggcgatcgca tgggcaggcc aggtagtcct
    28921 cgattgacag cgtttcgccg ttgccaaaaa ccagttcagc atccagtgcc agcaggacgg
    28981 gaagcagcac cgactcttcc tgccgggtgg cgatttcacc accaatggtc gactgattac
    29041 gaacatggcg tgagtaaaca aaaccgaggg cttcacgcag tgctgcagga ataaatcgtg
    29101 catcacgcag tggctgcaag cgagacattg cgccaattcg tagtgcaccg ttatcccagt
    29161 caatccagtc cagctccaga tcctgcaagg aaatggcaat ctttttatcg gtacgggttg
    29221 gtgtagcgtt gagtttgctg cccccggcga accagacggc ttcatcctgg tagcggcgct
    29281 tcagttccag cgcctgttcg acagagtcgg gtctgaaaaa ttgttcaatc atcgtctttc
    29341 cttaatttcc cctcttctct gaaagaagag gggagctgtt atttatgcca gcgcatccat
    29401 ccgacgccac atactggcag cggcttttct ggcttgcgca taaatggaat cgcaatcgaa
    29461 gttaaactga cggtcttcat agaccatcac accattgacc atcacgctgt gaacgctgcc
    29521 tgagcccata ccgaaagcga tatgcccggc gatattgtct gccagcagcg gcgttggcga
    29581 gttgtaatcg caaatggtta aatcggcttt gtaaccggct tccagaagcc caaatttcgc
    29641 gccaaagttg cggctcatca gttcgttacc gttagtcagg gctttggcaa aactgtcagg
    29701 ccacagcggg ccacccgcat cgcgatgttt aaagaaggca aatttcatct cttcaaacat
    29761 gtccgaacca atgccgtccg ttcccaacgc cagattgcgg atgtcgccaa ggtgatggtt
    29821 gtagccgaca tggttgttca tgtttgaacg ggcgttatgc accaggaacg catcgcgctg
    29881 attgagtagg gcgatgtcat ctttcgacaa gtacagccca tgagcgacca gcgttttgct
    29941 gccgatgaga tcgaattgcg ccagtcgtgc cagcaggtct ttgccgtacc aatggtgact
    30001 gtaggaaacg tcgtaaaggt cttccgcagc gtgaatatgc aaaccacgac ctgtgctttt
    30061 cacggcttca cgcagcatct ccagaccggc atccggtacg gtaaacggcg cgtgagcgcc
    30121 gatatgtgct tccaccagat acggctcggt tgctgctttc ttcgcctcat caatctgacg
    30181 ggcgaagcgg atgttttctt ctacaccttc ctgcaactct ttgatgccgc tgttacggtc
    30241 agtagtttca aaacaggtca tcgcgcgcag gccaactttt aaaaatgcgt cgcgcaatgt
    30301 ggagagcgac ccgccgatat aggctggaga ggcatggtga tcgataaccg atgtacatcc
    30361 gctcttaatt gcttccaggg aacaaatcag tccgctgtaa tagagcgact cttcatcaag
    30421 ggcgcgatcg agccgccacc agagattttt cagcgttgag atgaaatccg ggcagggggc
    30481 gatgtttgcc ataattccgc gggacagccc cgagtaaaaa tggttgtgcg agcagacaat
    30541 tcccggcatc acaatccgac catgcatctc tttgtagctg gcatcgggat agcgttgcgt
    30601 cagggcatcg tcgatagcga caatcacatc attttcgatg gcgatatcaa cgctttcctg
    30661 cactttcgcc gggtgtaact gcactgcggt gacattcttc agaatcaaca tgattacacc
    30721 tccacgcggc ccagcagata atgatgatgc tggtggacat ggctaataat gcggcacata
    30781 tcgttcagct ccggtggtac gttgttgaac tgaccttcgc tgtcgatgtt taacacccag
    30841 ctttggttat tcagacgtac tcgtacccgg caatcttcca caaggaagcc agggttgctg
    30901 ctgttatcaa agtcttgcga caggctgaag acggtgattt tgtctttgta cggtttaccg
    30961 ttccacggac agaactgagc gcagttgccg cattcgttac agtaagcgtc gaggtgcagc
    31021 gtctggaaac ggttctggaa gccagggacc gcaatggata cgttggcgcg gttcgggcag
    31081 acatccacac acttgctgca aacgtagtta cattcaaggc agcgtgcggc ttcctgggcg
    31141 acaaacgcat cacgatcgtc actgttcacc agagtgacag agatatcgcc tttacgttga
    31201 tagatttccg ccggattgac gttattccag tatttatcgt tctggtggga acggatattt
    31261 tcccgactca ggatggcatc ggtcgcccga cgcgcggttc cgacagcagc gacaatggag
    31321 gatggtccgc gctgtacgtc gccgatcata aagacgtcag tcagacgtgt ttcgccatta
    31381 tggtcgacgt ctggccagcc gtttttgtcc agcggcacgc ccattgcatt cagggcttca
    31441 gtatcctgct gttcaccaat ggcggtgatc aggctgtcta catgcagcgt tactgtttcg
    31501 ttggtttcta ccggacggcg acgacctttc tcatcaggtt cgccaagcga cataacgcgc
    31561 aaggttaagg tgccatcagc atcgaaacgt tccggattat tcagaaaacg gaactccacg
    31621 ccgtcgtgca acgcttcttc atactcttcg cgccatgctg gcatctcttg cagtgaacga
    31681 cggtaaacga ccgttgcttt ttctacgcct ggaacgcgta acgccgcacg agcgcagtcc
    31741 attgcggtgt tacccgcccc gacaacgacc acatgtttgc ccagcttgag cgcggtgccc
    31801 ttgttgtatt cacgcaggaa ggggagtgat ttccagacat tttggttgtc gcccgccagt
    31861 ttcacaccgc tatttttatc ggtaccggtg gcaatcagaa catagtggaa gccctgattt
    31921 tttaactgtt cgacggtcag atccggtgag caaccgtact caaatttcac gccgtgatcg
    31981 gcaacaaaat cgatatcgtg ctgaattaac tctgcaggaa tacggaactg aggaatgata
    32041 tttttcacca cgccgcccgc attggcttct cgttcaaaca gcgtaaccgg atggcccgct
    32101 ctggcaagga agtaacctgc cgccagacct gccggacctg caccaatcac ggctaccgga
    32161 tggcgtgaac cggaaccggc tggtttgtgc cagcgttgct tatattcatc ccaacctttt
    32221 tccagggcga cttttttcag ttcgcggata ttcagcgcac tgtcgtaatc caggcgggta
    32281 cagttgtatt ggcactggtg atcgcaaata tgaccggtaa tggcgggcag ggcgttgcgt
    32341 tgatagatga gttccagcgc gtcggcatag cggtgttcgc caagcaaacg gatgtattcc
    32401 ggaatatctt gcttgatagc gcaggcagta acacaggggg caacgtagca atcggtcagc
    32461 ggcaggtctt cagccacttc aatacgctct tctggcttcc agtgtttctg ggtgtattcc
    32521 atggttaacg cgtctgctgc cagtctgttc agtcgttcga cgtcaacgtg gttaagctcc
    32581 caggcgtcgg agccttccag ctcgcgcatg caggcactta agcgcagata gccgccaggt
    32641 ttcagcaggt cggttgccat agtaataggg cgaataccag tatcaaaaat atcgcggata
    32701 gtcagctgac tggcaccacc ggaataagaa atgggcagtt tgccgtcaaa ggcgcgagag
    32761 agaactgctg caacgttgat ggagagcggg aacagcgcac ggcctgacat atacatctct
    32821 tcaccgggca atgcgccttt attgttgatg gtgccgagag tgttagtcag ttttacgcca
    32881 aagccgagtg atttttcttt tgccagtgcc atcaggcgtt ccagcatttc cagtgcttgc
    32941 gtcagcttga ggtcgtgatc aaatgactct tcttttaagc cgatgtaacc gaaaccgcag
    33001 acatcgagga tctcacgaac acgcgcgtac cccagtaagg tcgggttgag tttcacaaag
    33061 gtgttgagcc ctttttcttc cagcatgtag cggcaaatgg cttcgatttc atgcggagga
    33121 cagccgtgca tggtggagag ggtaacccct tgtaccatgc tggtggggat gcgagcgggt
    33181 aaggcttgca agctttcgcg tttttcctgc aatccgtggc gggctaaaaa tgcctcatcc
    33241 tggagtagct tattaagtgc atcgcggtat tgagcgaatt tcggatggtc agatgcgtcc
    33301 atcatattgt cgatgaactg ctgcatcggc ggttgcttaa taccttcgag gttgtaaccg
    33361 acgctcatat taaagatgaa cgatttaccg gaatcagaag gctggaacat cgcttcgaga
    33421 aggtgcaggg caaaccaggc tttgaggtat tcatcccagg ctttaagcag ggtaaattcg
    33481 gtagaccatt cggtgttaaa gcactcgtct tcggcatcga tacagggctt ttccagctcc
    33541 aggcggtcaa gaatttggac ggtttttagt tcgatgaagc gtccgccagt cagccaggag
    33601 gtgacgatgt tttgcgcaag ttgcgtgtgc gggccagcag cagggccgac gggagtggca
    33661 caggtttcac cgaagacgct aacagtttta ccttttacgg gtgagtaaaa ctgttgctcg
    33721 ggaataccaa agattgagcg ttgttgttgg tattcatcaa atatgcgcgt caaaagttcc
    33781 tcaaacggaa tgggacgcat aatatccccc ataaccctct ccttagctta tacagatatc
    33841 attgagtgac cggtgttgag aaatccggtg gtttaggggg atagagcaac aatcgcacca
    33901 ctcttgccag gtttggcggg aaatataaaa aaatgtgagg cggctcgaaa cttaatggct
    33961 ttttgtgaat gaactcacag gtgctaaatg agaattaaaa tcactcctaa aaattattat
    34021 tgccagcaca tttatcactt tttctctttt ctcaaaatca gtgattcctt atcattatga
    34081 taaagagtgc aggagggatt aaatatttga acgcaatcgt ttaactacgt tttctaaatt
    34141 tcagtatacc ttttcttcgc tgtaataaag tcgtccggag tatcaatatc taaaattatt
    34201 tctgcatttt ctatttcgac ggaataatgt tctcccataa ggagtgcctg acgcatattg
    34261 gtgacattgg gtcgctggat tgcctgcatc aggcatggtt ttgacactaa aatcggatgg
    34321 ccggggatgc cattatggag tggcagtatt gcgccatcat ttcgtaacga ccagattttt
    34381 ctaaaaatat cgatggtgag ggttggcata tctccgtggg tgagaaaaca atgttctgtt
    34441 tgcaccgcgg gtactgcggc ctttactgat gtcagtaaac cctgcgcata atctgggttg
    34501 tggataatag tgatattgct ctggttcgca taacgttcgt gcagttcatt accacgatag
    34561 ccggtgacta aaataattcg gctacaaaac tgcaacgcat ttttgatact tgtatcaaga
    34621 attgttccct gttgccaggg taacatcatt ttccattgcc ccattcttga tgataatcct
    34681 gcggcggtaa ttatacagtc gatggctgac atagtgattc cattatttac aggagctaat
    34741 aacgcctgat aattgcgtta cttcctgatg gattaagttg agggtaattt atcccagaga
    34801 ggtccataaa gacgcgtgaa aagtataatt gacccatcgg cattattcat tgatttaggc
    34861 gcgcagaaac gtcccactgt gatttctatt gttggtgctg gggggaaaac cagcttgctt
    34921 ttttggctgg cagaactgtt ccaggcgagc ggcaggcgtg tattaatcac tacgacgaca
    34981 catatgttta tgccaacatc tcactggccc gtggttttct gtcgtgatcc cgccatgctt
    35041 cctcatgcgt cttttatatc ccccatttca ttttgttttc acagctggaa agcgaaccag
    35101 ggaaaagtgc agggatttac gccagaagcg attgatgcac tggtacaacg accagagtgt
    35161 gacgtaattc tcattgaggc agatggctcg cgtggaatgc cgttaaaagc gcctgatgag
    35221 cacgaacctt gcatacctaa aagcagttgc tgcgtgattg ctgtgatggg aggacatatt
    35281 ttgggtgcga aagtgagcac agaaaatgtc catcgctggt cgcagtttgc tgatattact
    35341 gggttaacac ctgatgcacc cttgcaactg agcgatctcg ttgcgctggt tcgccaccct
    35401 cagggggcgt ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat taaccgtttt
    35461 tctcaatgtg agaatgcgat tgcgcaaatc gagctccttc aaccgctgca acaacacaac
    35521 gtagaggcaa tctggctggg cgatatacaa gaacatcctg caatcgcgcg cagatttgtg
    35581 aattagcggc ccgggtattc cgggttactg aaaggatatc cattgaggtt ggtatgaata
    35641 ttttcacaga ggctgcaaaa ctcgaagagc aaaattgtcc gtttgcgatg gcgcaaattg
    35701 ttgatagccg aggctcaacc ccccgccatt ctgcacaaat gttagtgcgc gccgatggtt
    35761 ctatcgtcgg tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa gagtcgcttc
    35821 aggcattgca ggaacgtaag ccgcgattat tccatggacg tatggctcgt aacggtgcgg
    35881 atgctgtcgg gtcagattgt ggaggtgcaa tgtcagtatt tatcagcgtc catggtatgc
    35941 gtccacgtct ggtgttgatt ggtgcggggc atgtcaaccg ggcgatagcc cagagtgcgg
    36001 cgctattagg atttgatatc gccgttgctg atatttatcg cgaaagcctc aatcctgaac
    36061 tattcccacc atcaaccacg cttctccatg ctgagtcgtt tggtgcggca gtggaagcac
    36121 tggatattcg ccctgataat tttgtcctga ttgccacgaa taatcaggat cgtgaagccc
    36181 tcgacaaact cattgaacaa cccattgcat ggctggggtt gctggcaagt cgccgcaagg
    36241 ttcagctttt cctgcgccaa ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt
    36301 tacatgcgcc cgttggttac aacataggtg cggaaacgcc gcaggagatc gccattagcg
    36361 tgctggcaga aatattacag gtgaaaaata acgcgccggg tgggctgatg atgaagcctt
    36421 ctcatccttc cggacaccag ctggtggtga ttcgcggtgc gggggatatc gccagtggtg
    36481 tagcgttacg tctgtatcat gcgggtttta aagtgatcat gttggaagtg gaaaaaccga
    36541 cagtgattcg ttgtaccgtg gcgtttgccc aggccgtgtt cgatggcgaa atgacggtcg
    36601 aaggcgtcac cgcccgcctg gcaaccagct ctgcggaagc gatgaaactt accgaacgcg
    36661 gatttatccc tgtgatggta gatcccacct gttcattgct tgatgaactg aaaccgcttt
    36721 gcgtggtgga cgctattctg gcgaaacaga atttgggaac gcgggcagat atggcaccag
    36781 taacaatcgc gcttgggccg ggctttactg cagggaagga ttgtcatgcg gtaattgaaa
    36841 caaatcgcgg gcactggctc ggtcaggtga tttactctgg ttgtgcgcag gagaataccg
    36901 gtgttcccgg caatattatg gggcatacca cccgacgggt gatccgtgct cctgctgcag
    36961 gcattatgcg atccaacgtg aaattaggcg atctggtgaa agagggcgat gtcattgcct
    37021 ggattggtga gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg
    37081 acggactggc cgtggtcggt ggttttaaaa ttggtgatat cgatcctcgt ggtgaaacgg
    37141 ctgatttcac cagcgtttct gataaagctc gggcgattgg cggcggcgta cttgaggcgt
    37201 taatgatgtt gatgcatcag ggcgttaaag cgacaaaaga agtgctggaa gtggcttaaa
    37261 tgaaaatggc cggatggtat tacccatccg gcctttaaat tactgacaaa tcaccgttcc
    37321 ggttttgcct tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc
    37381 tgcgcgagag cgagcaaacg aggcggcagc ttccacttta ggcagcatag aacctttcgc
    37441 aaaatgacct tcttcgataa agcgttcagc atcacttaac gacaggcgat cgagccactg
    37501 ttcattctct tttccgaagt taatggccac tttttctacc gcagtcagaa tgatcagcat
    37561 atcggcatcg atcatttctg ctaaacgggc gctggcccag tctttatcga taaccgcgct
    37621 ggcaccacgc agatggttac cttcacgaat aacagggata ccaccaccgc caacggtgat
    37681 caccacctgt ccggcatcta ccagagcttt aaccgtttct ttttcaatga tatcaaccgg
    37741 ttttggcgaa gcaacgacac gacgatagcc gcgacccgca tcttctttca gagtgtaacc
    37801 ctgttttgtc agtagctctg cttcttgctc ggtaaagaac gaaccaatcg gcttggtcgg
    37861 gttgaggaat gccggatcgt tagcatcgac ttcgacctgg gtaaccagcg ttgctacagg
    37921 tttattaatg ccacgagaaa gcagttcttc tcttaaggcg ttttgtagat cgtaaccaat
    37981 ataaccctgg cttaaggcaa cacagacaga catcggcagc attggcgagt gcgcttcagt
    38041 ttttgctgca gcttcaaagg cctggttaat catgcccacc tgtggcccgt tgccatgagt
    38101 gacgatgacc tcatgtccct gggcaattaa atcaacaatt gcctgagaag taatttttac
    38161 cgctttcatt tgcccagcca ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat
    38221 tttcttactc atttttcctc actcatcatt cttgattgtg tatagatccc tggcagtggt
    38281 tagagcacgg gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct
    38341 gtaaaggtgc cgtcgcagaa aatcgtttca ccacgagata aggttctgac aatcgcgccc
    38401 tgacaggtaa aaccctccca tggcgagtag tcagcgttgt cgtggagatg gcgatgctga
    38461 atttgttggc tctgacgtgg gtctataatc accacgtcgc catcggaacc gggcgctaat
    38521 aatccttttt gtggccacag gccaaacagc ctggcgggca ttgcactggt taactcaaca
    38581 aaacgctccg gtgagatacg tcccgtcatc acgccactgg agaacagcag ttgcatgcgg
    38641 ttctccacac cgggtaagcc atttgggcag cggctgaaat cgcctttaga aatttgcagg
    38701 cgttgagcca tcgagaaggt gcagtgatcg gttgccacca cgtcaatcgc accatcgctg
    38761 atgccacacc acagtttgtc ctgctcgcgt acgttacgca gcggtgggct aagaatgaac
    38821 ttcatgccat cttctgtatc gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt
    38881 tcaacccaga ctggctggtg attcgcacgg gcaagacgca gataatccag acctaagccg
    38941 ttagacaggt gcacgatata gagcggggcg ttaccggcaa tttgtgccag gttaatcatg
    39001 cgggcgatgg cttccgcttc gcattccaga ggtcgactca aggcatgata acgcggcgcg
    39061 gttaaccctg cggcgataaa ctccgcccgc ttgctggcga tagctgcgtc attttccggg
    39121 tgcacggtgg tcagcgcgcc ggattcatgc aagcggcgta atgcctgcaa cacttcatca
    39181 tcgttgagtt tatattgata ggttaaatag agtttaaaac tgctcagtcc ttcctcaacc
    39241 atcatcggaa tttcgtcgag gatagcgtga ttaatatgtt gaatgacacc gtgaaagctg
    39301 tagtcgatga ctgctttgtg ggcggcataa ccgcgataaa cttccagttg atggcgtaac
    39361 cggcagccat ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc gccacacgca
    39421 gcagcgcggg taccggtaaa aaaatcatca caactgcgcg cgatgccgac atcaatattg
    39481 aaatgcgtat ggacatccac gccgccaggg aaaacgtaac agccagaggc atcaatttct
    39541 tcgcaaggga gttgcggcga aatatcggta cccaactggc gaacaatccc gctttcaatc
    39601 agcaaatcct gcttcgcctg tccatctgca ttaacaacaa tgccattttt tatcaatacg
    39661 cgcatagcaa actccaaaaa ccctccggcg aaccggaggg gaataagtga agagtaatta
    39721 ttcggttgcc agccagctta atgggattgc agcgtacata gcggcacagg ttaccaggtg
    39781 agatttccag gttttttcgt ttggcgcgtg cgcttccggt tctttaccag ggccaaaacc
    39841 gatgaccgga atgccgtggc gacccatgat ggatacgcca ttagtcgaga aggtccactt
    39901 atcaacgaca ggcgctttac caaacagccc ttcataagca ttcaccagtg ctttaacggt
    39961 gaagtgatct tcttccactt tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc
    40021 agtccaggac gggcggtcgt agttgtacat agaaacaacc gcgttagctt tctgtactgc
    40081 aggtagagcg cggatctcgt ccagcgcgcc ttcccaggtt tcgccccagg tcagacgacg
    40141 gtcgattgaa actgcgcagc tgtctgctac agcgcaacga cttggggagg tgaagaagat
    40201 ttcagaaaca gtcagcgtac ctttgccgag gaattcgtca tagcccagac gttgagacag
    40261 ttcctgtaat tcgccaagaa ttggacccat tttgaaaatg gcgttatcac cacgttctgg
    40321 tgccgaaccg tggcagctaa caccctgaac atcaatgcga atttccatac gaccgcgttg
    40381 accacggtat acctggcagt cggttggttc ggtactgacc acaaattccg ggcgaatgcc
    40441 ggattgttca ataatgtatt gccagcacag accgtcgcag tcttcttcct gcacagtacc
    40501 ggtgaccagc agggtatatt catcttccag accgagatct ttaatgattt ttccggcata
    40561 taccatagac gccatgccgc cttcctggtc ggaagtacca cgaccaccga tcagctcgtc
    40621 ggtttccatg ccttcatacg gatcgaagtc ccagtttttg atgttgccaa tgccgactgt
    40681 atcgatatga gcgtccattg ccaccagacg cgggccgtgg ccgatataac cgagaacgtt
    40741 gcccattggg tcgatttcaa ctttatcgaa gccgactttt tccatctctt ctttaatacg
    40801 atgtactacg cgtttctcgt cgcagctttc actgggaata gcaaccatat cgcgcaggaa
    40861 gcgagtcatg tctgcctggt aatcttttgc tttttcaaga atcagtttga atggaatatt
    40921 cttagccatt atttttctcc agatttttta ctccccgttt tgcagggagc agttccaata
    40981 gggtgattaa ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac
    41041 gtcggtgtcg ccttcagtgc tgataaccag caccacggca tctttgttca gcgccagttt
    41101 ttccatcagg ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac
    41161 agcgccggat tcaccggaga tgatgcgcgg gtcgttgcca tacggattac ccagcacgcg
    41221 catacctaat gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc agttacgtag
    41281 gatttcccag cccagtgggt taggttcgcc gcaggccagg cccgccatga tggtggccat
    41341 atcaccgcca acgttgacga tgtcgccttt gacgccggag cgataaatac agtcagcttt
    41401 gtcaggttca acaataatgc tgtgcagatt ttgcgggcta tagacgtcga ccagataacc
    41461 cagcacgcca ccggccattg caccgacacc ggcttgcagc agaacgtgtg tcggggttac
    41521 gcccatttca cgcatttgct cgacggcttc atccgccagt gttgcgtagc cttgcatgat
    41581 ccaggttggg attttggtgt agccttccca tgccgtgtcc tgtaccactt cccagccgtg
    41641 ctgctgcgcg tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat ccgtgacgat
    41701 gcactcggca ccgaggttta gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat
    41761 gtaaatcacc gcattctgtc cgagttgctg tgctgcccat gccacaccgc gcccgtggtt
    41821 gccgtcggtg gtagtcgcga aagtcatttt ttcgccgatg gcatttttca ggtgctcaaa
    41881 tgacagcgtt tcgatatcaa gatgatattt ttcacacaat aattgagcga tggcgtacgc
    41941 accgccgagc attttgaagg cgttcagacc gaatcgtttt gattcgtctt tgacgagaat
    42001 tttctttaca ccaaacaggt ttgcgagatc gtccagcgca caaagcggtg ttgggtgata
    42061 accagcaatt ttctggtgga actggcgtgc cagttttgcc tggctttgcg aaaaaagcgg
    42121 tgatgtttcg ccgttgaaaa atttgttatc ggcgatatca atcttcaatg agaaaacgga
    42181 catatcctat ccttttggcc tctggaaata gagggccgac cagctgatcg gcccgagggg
    42241 ttatttcacg cgttcttgcg cttctttcag cagttgctcg agcagtgcac ctggtttggc
    42301 gtatttacgg gacaggatca tcgcggcgat gatataaggc ttccagctgg cttctttgta
    42361 ggtagcgata cggtattttt cgaatacgcc ttcagtcact tcaccttctt tgcaggatac
    42421 gccgctgata tcagctggca ggcagtgcat gtacagggct tcgccatcac gggtcagttc
    42481 catcatctct tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca gacactgttt
    42541 ttccagtgct tttaagcctt cgtgatcgtt cgcacgcagc aattcagtac gctcttccat
    42601 cactttgtaa ggtgcccatg acttcggata aacgatgtct gcgtctttga aggcttcttc
    42661 catgctggtg acctgacgga agctaccacc ggaggcttta gcattgtttt tagcaacttc
    42721 aacgacatcc gggatcaggt cgtagccttc cggatgggcc agggtgacat ccataccgaa
    42781 gcgggtcatc agaccgatga tgccttgtgg tacagagagc ggtttgccat agcttgggga
    42841 gtaggcccag gtcatggcga tttttttacc tttcaggttt tccagtgaac caaagtgttc
    42901 acgtaaccat gccagatcag ccatagactg agtcgggtgg tcaatatcgc attgcaggtt
    42961 cactaaagcc ggacgctgtg gcagaacccc ttgtttgtag ccgtcatcaa gtgccgcgcc
    43021 aacttcacgc atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa taccgatagc
    43081 gtcagcgcag aaggagatca tattggcggt ttcacgcacg gtttcgccgt gagcgatttg
    43141 tgatttacct tcgtcaagat cttgttgtgc aaggccgagc aggttaagcg cggaagcata
    43201 agagaagcgg gtacgggtgg aattgtcgcg gaatacggaa atacctaatc cactattaaa
    43261 gactttggtt gagatgtttt cagcacgcag tgcttttaac gcggcggcaa cgtccagtac
    43321 ttgtttcagt tcgtctggcg tctgttccca cgttaacaaa aaatcttttt catgcaggtg
    43381 agaggtcagc gaattgatat ccttaatcag ctcattaaca gttttcatca taaagtcccg
    43441 atagtggaat gacaggcaaa gtggtttatt tcactgtgac gtgccaaagt gattggtgtt
    43501 ttcacagcta tacctgggac taacaataag tgtgccagaa ttatccacgc ggctgcgaaa
    43561 ggctaatttg acggataatg tgtggggttg atcacatatt gatgccagtt tccggttgtt
    43621 tgtaaaatac gaaaacgttt ggcttttttt atcacgttat ttatttgctg ctaagtatca
    43681 gaaataagat aaagaggcag gctatctctc ttcagtttga taaactt
//
LOCUS       DADSZD010000018        43129 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.018, whole genome shotgun sequence.
ACCESSION   DADSZD010000018 DADSZD010000000
VERSION     DADSZD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.018"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(49..1332)
                     /locus_tag="J1Z28_000893"
     CDS             complement(49..1332)
                     /locus_tag="J1Z28_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBA7715749.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(1445..3379)
                     /gene="yeaG"
                     /locus_tag="J1Z28_000894"
     CDS             complement(1445..3379)
                     /gene="yeaG"
                     /locus_tag="J1Z28_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBA7715750.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            3815..4561
                     /locus_tag="J1Z28_000895"
     CDS             3815..4561
                     /locus_tag="J1Z28_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MltA-interacting protein"
                     /protein_id="HBA7715751.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            4651..5505
                     /gene="yeaE"
                     /locus_tag="J1Z28_000896"
     CDS             4651..5505
                     /gene="yeaE"
                     /locus_tag="J1Z28_000896"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBA7715752.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLITQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(5556..6440)
                     /locus_tag="J1Z28_000897"
     CDS             complement(5556..6440)
                     /locus_tag="J1Z28_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBA7715753.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WALKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(6524..7519)
                     /gene="gapA"
                     /locus_tag="J1Z28_000898"
     CDS             complement(6524..7519)
                     /gene="gapA"
                     /locus_tag="J1Z28_000898"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA7715754.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            7861..8274
                     /gene="msrB"
                     /locus_tag="J1Z28_000899"
     CDS             7861..8274
                     /gene="msrB"
                     /locus_tag="J1Z28_000899"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBA7715755.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPLSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8316..8588
                     /locus_tag="J1Z28_000900"
     CDS             8316..8588
                     /locus_tag="J1Z28_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBA7715756.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            8958..10034
                     /locus_tag="J1Z28_000901"
     CDS             8958..10034
                     /locus_tag="J1Z28_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA7715757.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHSLWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            10061..11440
                     /locus_tag="J1Z28_000902"
     CDS             10061..11440
                     /locus_tag="J1Z28_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715758.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            11457..12500
                     /locus_tag="J1Z28_000903"
     CDS             11457..12500
                     /locus_tag="J1Z28_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HBA7715759.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            12521..13357
                     /locus_tag="J1Z28_000904"
     CDS             12521..13357
                     /locus_tag="J1Z28_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA7715760.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAIKERALDKIKLFGSDGK
                     AE"
     gene            13362..14309
                     /locus_tag="J1Z28_000905"
     CDS             13362..14309
                     /locus_tag="J1Z28_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBA7715761.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            14319..15299
                     /gene="ydjG"
                     /locus_tag="J1Z28_000906"
     CDS             14319..15299
                     /gene="ydjG"
                     /locus_tag="J1Z28_000906"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBA7715762.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGE
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            15436..16194
                     /locus_tag="J1Z28_000907"
     CDS             15436..16194
                     /locus_tag="J1Z28_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7715763.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLTDFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            16312..17670
                     /locus_tag="J1Z28_000908"
     CDS             16312..17670
                     /locus_tag="J1Z28_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7715764.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKRIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(17763..18404)
                     /gene="pncA"
                     /locus_tag="J1Z28_000909"
     CDS             complement(17763..18404)
                     /gene="pncA"
                     /locus_tag="J1Z28_000909"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBA7715765.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(18415..19431)
                     /gene="ansA"
                     /locus_tag="J1Z28_000910"
     CDS             complement(18415..19431)
                     /gene="ansA"
                     /locus_tag="J1Z28_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBA7715766.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPVPHGEGALIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(19598..21454)
                     /gene="sppA"
                     /locus_tag="J1Z28_000911"
     CDS             complement(19598..21454)
                     /gene="sppA"
                     /locus_tag="J1Z28_000911"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBA7715767.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            21615..22166
                     /locus_tag="J1Z28_000912"
     CDS             21615..22166
                     /locus_tag="J1Z28_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBA7715768.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAVGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            22283..23326
                     /gene="selD"
                     /locus_tag="J1Z28_000913"
     CDS             22283..23326
                     /gene="selD"
                     /locus_tag="J1Z28_000913"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBA7715769.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            23331..25286
                     /locus_tag="J1Z28_000914"
     CDS             23331..25286
                     /locus_tag="J1Z28_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBA7715770.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGRE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(25363..26706)
                     /gene="gdhA"
                     /locus_tag="J1Z28_000915"
     CDS             complement(25363..26706)
                     /gene="gdhA"
                     /locus_tag="J1Z28_000915"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013366584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBA7715771.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            26942..27214
                     /locus_tag="J1Z28_000916"
     CDS             26942..27214
                     /locus_tag="J1Z28_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBA7715772.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(27180..27587)
                     /gene="nudG"
                     /locus_tag="J1Z28_000917"
     CDS             complement(27180..27587)
                     /gene="nudG"
                     /locus_tag="J1Z28_000917"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBA7715773.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            27674..28294
                     /locus_tag="J1Z28_000918"
     CDS             27674..28294
                     /locus_tag="J1Z28_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBA7715774.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(28303..29610)
                     /locus_tag="J1Z28_000919"
     CDS             complement(28303..29610)
                     /locus_tag="J1Z28_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBA7715775.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(29677..30330)
                     /locus_tag="J1Z28_000920"
     CDS             complement(29677..30330)
                     /locus_tag="J1Z28_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBA7715776.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPVLDMAQWSENYNKLR"
     gene            complement(30330..31865)
                     /locus_tag="J1Z28_000921"
     CDS             complement(30330..31865)
                     /locus_tag="J1Z28_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715777.1"
                     /translation="MATPLRYALIFLLWAMIAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLIFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWQEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(31838..33004)
                     /locus_tag="J1Z28_000922"
     CDS             complement(31838..33004)
                     /locus_tag="J1Z28_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7715778.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQTLLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(33014..33562)
                     /locus_tag="J1Z28_000923"
     CDS             complement(33014..33562)
                     /locus_tag="J1Z28_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA7715779.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(33562..34269)
                     /locus_tag="J1Z28_000924"
     CDS             complement(33562..34269)
                     /locus_tag="J1Z28_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA7715780.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(34283..34960)
                     /gene="ydjY"
                     /locus_tag="J1Z28_000925"
     CDS             complement(34283..34960)
                     /gene="ydjY"
                     /locus_tag="J1Z28_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBA7715781.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(34965..35675)
                     /locus_tag="J1Z28_000926"
     CDS             complement(34965..35675)
                     /locus_tag="J1Z28_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBA7715782.1"
                     /translation="MNAERKFLLACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLMARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(35842..36648)
                     /gene="xthA"
                     /locus_tag="J1Z28_000927"
     CDS             complement(35842..36648)
                     /gene="xthA"
                     /locus_tag="J1Z28_000927"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBA7715783.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            37094..38314
                     /gene="astC"
                     /locus_tag="J1Z28_000928"
     CDS             37094..38314
                     /gene="astC"
                     /locus_tag="J1Z28_000928"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBA7715784.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            38311..39345
                     /gene="astA"
                     /locus_tag="J1Z28_000929"
     CDS             38311..39345
                     /gene="astA"
                     /locus_tag="J1Z28_000929"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBA7715785.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRVIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            39342..40820
                     /gene="astD"
                     /locus_tag="J1Z28_000930"
     CDS             39342..40820
                     /gene="astD"
                     /locus_tag="J1Z28_000930"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBA7715786.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGERRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGLPDEEVFGPLLRVWRYDNFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            40817..42160
                     /gene="astB"
                     /locus_tag="J1Z28_000931"
     CDS             40817..42160
                     /gene="astB"
                     /locus_tag="J1Z28_000931"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBA7715787.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFRGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPATESLLKAIFNDEEKFA
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVSGFMAIEVPTAQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFNLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            42153..43121
                     /gene="astE"
                     /locus_tag="J1Z28_000932"
     CDS             42153..43121
                     /gene="astE"
                     /locus_tag="J1Z28_000932"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBA7715788.1"
                     /translation="MDNFLALTLTGKKSVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT        10532 a        10543 c        11370 g        10684 t
ORIGIN      
        1 tacagtgagc taaaaatcag ccaggcgata atgacccggc tgacagtttt agtctttagc
       61 tgttgcattt tgtttatgaa acagttcacg gaacaccgga taaatatcat cctggtcgcg
      121 gatatgctgc atcgcaaagt tgtcgaaagt agattgcaga tgctcatatt ctcgccacag
      181 tgtctgatgc gcgcgacgtg taatttcgat atagctgtaa taacgaacaa cgggtaatat
      241 ttttttcgcc agaatttcat ggcaaagcgg agagtcatcg gcccagttat cgccgtctga
      301 tgcttgggct gcgtaaatat tccactgtgc cgggttataa cgctctttca ctacctcatc
      361 catcagtttc agggcgctgg aaacaatggt gccgccagtt tcctgcgagt agaaaaactc
      421 atgttcatcg acttctttcg cctgggtatg atggcggatg tatacgactt ccacgttctt
      481 atacgttctg ctgaggaaca gatacagcag aatataaaaa cgtttagcca tatccttagt
      541 ggactgatcc attgaaccgg aaacgtccat caggcaaaac atcactgcct ggctggaggg
      601 atcgggccgt ttttcgtagt tcttgtaacg taaatcgaag gtgtcaatga aagggacgcg
      661 ttcaattttg gcacgtaatt ctgcaatttc tttgcgcaga cgttcctctt ccagcagttg
      721 cgcaggttca ctgttgctga tgatggccaa attctcttcc agtgcatgaa gttctcgccg
      781 cttgcccgcc gtcatagctg tgcgccgcgc cagtgagttc tgcaatgaac gtacaacgct
      841 gatattggct ggaacgccgt tagcggtata acccgcccga tgcgttttat attcggtcag
      901 ctggcgttgt tggttttgtt tcagattcgg taaggccaaa tcttcaaaga gcagatcaag
      961 atactcatct ttcgaaatct gaaagacaaa ttcatcctga ccttcaccat cctggctggc
     1021 ctggccctga ccactgccgg aacctccgcc accaccctgg ggacgttcaa ttcggtcgtt
     1081 ctggacgaaa tggtcattgc ccggatgtac gcggtggcga aggccaccac gcccctgatg
     1141 aaacatcggt tcgctaatat cttccgtggg aatggatacg gactcgccgc tgtcgacgtc
     1201 agtcaccgaa cgcttattta tggcctcgga gatcgactgt ttaatttgcg ctttataacg
     1261 gcgtaagaaa cgctggcgat tcaccatgct tttatttttg ccgttcagac gccggtcaat
     1321 aaaccaggtc atatgccccc cgtactacat ttgccaacgg tatgctacgc ctgtgttgcc
     1381 gcttctctgg cccacggttc gctgtcgttg taggcccggt agcatgtgta ccgggccagg
     1441 gttgttaaga cgatttacgt acgcgcaaat accattcgca cagtaaacgc acctgtttac
     1501 gggtgtagcc tttttccatc atacggtcga caaagtcgtc gtgtttcttc tgctcgtcgg
     1561 ttgacgtttt ggcgttaaac gaaataaccg gcaacagctc ctcggtatta gagaacattt
     1621 tcttctcaat aaccgtgcgc agtttttcat agctggtcca gttcggatta cgtccgctgt
     1681 tattggctct ggctcgcagt acgaagttaa caatctcgtt gcggaaatct tttggattac
     1741 tgatccccgc cggtttctcg attttctcca gctcggcgtt aagagactcg cggtcaaaca
     1801 gctgcccggt atccggatcg cgatactcct gatcctgaat ccagaaatcc gcgtaggtaa
     1861 cataacggtc aaaaatgttc tgcccatatt ctgaatagga ttcaaggtag gccgtctgga
     1921 tctctttgcc gataaactcg gcatatttcg ggatcagata acctttcagg aactccagat
     1981 agcgttctgc ctgctcttgc gggaactgct cgcgctcgat ctgctgttcc aggacgtaga
     2041 acagatggac cgggtttgcc gccacttcta catgatcgaa gttgaacacg cgggagagga
     2101 tcttaaacgc aaaacgcgtc gacagaccgt tcatcccttc atcgacaccg gcgtagtcac
     2161 gatattcctg atacgacttg gctttgggat cagtgtcttt caaactttca ccatcataaa
     2221 cccgcatctt tgaataaatg ctggagtttt ctggctcttt cagacgcgaa agaatagaaa
     2281 aacgtgacag cgtttccagc gtgccagggg cgcagggggc gtgagtcaat tcactgtgat
     2341 taagcaattt ctcgtagatt ttgatctctt cggaaatgcg caagcaatac ggcaccttca
     2401 cgatgtaaac acggtcgagg aaggcttcgt tgtttttgtt attacggaaa gtgacccatt
     2461 cggattcgtt cgagtgggcg agaataatcc cgttgaacgg cagggcggag ataccttccg
     2521 tcccgttgta gttaccttcc tgggtggcgg ttaacaaggg atgcagcact tttatcggtg
     2581 ctttaaacat ctcgacaaat tccatgatcc cctgattggc gcggcacagc gcaccggaat
     2641 agccgtaggc gtccgggtca ttctgcgcgt agtgttcgag tttacgaata tcgactttcc
     2701 ctaccagggc ggagatgtcc tggttgttct catcgccggg ttctgttttg gcgatagcaa
     2761 tttgttgcag aattgacggc cagaccttca ctacccggaa cttagtgata tcgccaccaa
     2821 attcatgcag gcgttttgcc gcccatggcg acatgatggt gccgagataa cggcgaggaa
     2881 tgccatactc tttttccaga atctgcgcat cttcctgcgg attgaaaaga cagaacggat
     2941 gatcgttgac cgggctacgc tcaccgttgg cgctcaatac ataaatcggt acgagctgca
     3001 ttaatgattt cagtcgctca gcaagcgatg atttaccccc acccacaggc cccagcagat
     3061 acaggatttg tttcttctct tccagcccct gagccgcgtg tttcagataa gagacaatct
     3121 gttcaatcgc gtcttccatg ccgtaaaact cttcaaacgc cggataacgt gcaatgaccc
     3181 ggttagaaaa gagtcgagaa agtctgggtt cctgggctgt atcgaccatg acaggctcac
     3241 caatagccat caatagccgc tcagccgcgt tggcataagc actgcgatct tgccgacaag
     3301 tggtaagaaa ctcctgcagt gtgaactctt cgtccttggc agcttcatat cgctggcgat
     3361 agtgatcgaa tatattcatg gtatgccgtc ctttcgtttt ttagcacagg ttaagagccg
     3421 ttcatatgaa tagttgaggc tcccggaaga ggtaaactga acgacgtctc ttgctaaaac
     3481 acagcaactc tcatgccatt ttattgacga tgagaacaac gtgcccggtg atacaccttc
     3541 aaaattaagc gtagatggca aatggaaaac ttgcctgcac ggctttctta atttcaatgg
     3601 catatcaaca actcatccat aaattttgca taattaatgt aaataatagg gtcgccggta
     3661 acgctaaatt catttggctg taagcgtggt gtcatctgcg tcaggaaaat taaacagtta
     3721 ctataaaaaa tgaaaacgta aaaaggttgg gtttcgatgt attgacgggt aaactttgtc
     3781 gcccgctaaa catttgttta aggaatgatt aattgtgacc aaactcaaac ttctggcact
     3841 tggagtgctt atcgcaacgt ctgcaggcgt agcgcacgct gaaggtaaat tttccctggg
     3901 cgcaggcgta ggtgtcgttg aacacccata taaagattac gataccgatg tttacccagt
     3961 accggtaatc aactatgaag gcgataactt ctggttccgt ggcttaggtg gtggttacta
     4021 cctgtggaat gacgcaacgg acaaactttc aattaccgct tactggtcgc cgctttactt
     4081 caaagctaaa gacagtggcg atcaccaaat gcgtcacctg gatgaccgta agagcaccat
     4141 gatggctggt ctgtcttatg ctcactttac ccagtacggt tacctgcgta ccactctggc
     4201 tggcgatacc ctggataaca gcaacggcat cgtctgggat atggcctggt tgtatcgtta
     4261 caccaacggt ggcctgaccg tgactccggg tattggtgtg cagtggaaca gcgaaaacca
     4321 gaacgaatac tattatggcg tatcgcgcaa agagtccgct cgcagcggtc tgcgtggcta
     4381 taacccgaac gacagctgga gcccttacct ggagctgagc gccagctaca acttcctcgg
     4441 tgactggagt gtttacggta ccgcgcgcta cacccgtctg tctgatgaag ttactgacag
     4501 cccaatggtg gataaatcct ggactggcct gatttctacc gggatcacct acaaattctg
     4561 ataatgcatt tttccagggc attttgtgca taaaaacagg gcgtcatgcc ctgttttgca
     4621 ttacggtggt gcaatacctg ggaggaagca atgcaacaaa aaatgattca atttagtggc
     4681 gatgtctcac tgccagccat agggcaggga acatggtata tgggcgaaga tgccagtcag
     4741 cgtaaaacag aagttgctgc actacgcgcg ggcattgaac tcggtttaac cctcattgat
     4801 accgccgaaa tgtatgccga tggcggtgcc gaaaaagtgg ttggggaagc attaatcggt
     4861 ctgcgtgata acgtctttct cgtctctaaa gtctatccgt ggaatgctgg cgggcaaaaa
     4921 gcgataaatg catgcgaagc cagtttacgc cgtctcaata ctgattatct cgatctctac
     4981 ttattacact ggtctggcag tttcgctctc gaagagactg tcgcagcgat ggaaaaattg
     5041 atcacccagg gaaaaatccg ccgctggggc gtttctaacc ttgattatgc cgatatgcag
     5101 gaactctggc agctgccggg gggaaatcag tgtgccacta atcaggtgct ttaccatctc
     5161 ggttcacgag ggattgagta cgatctactt ccctggtgcc agaaacagca gatgccggtg
     5221 atggcttaca gtccattagc ccaggccggg cggttgcgca atggactgtt aaaaaatgcg
     5281 gtagtcaacg aaattgcaca tgctcacaat atcagtgcgg cacaagtatt gttggcgtgg
     5341 gtaatcagcc atcagggtgt gatggcgatt ccaaaagcgg caactgtcgc acatgtccaa
     5401 caaaatgcgg cagtgcttga ggttgaactt tcgtcagcgg aattagttat gctggataaa
     5461 gcgtatccgg caccaaaagg aaaaactgcg ctggatatgg tgtgatgttg atacgtgccg
     5521 gatgcatcgc acccggcaca caatattaac gcaaattaac gtttcgcaac gcgaatggat
     5581 tgcgccagat gtgcaggttt ctctttggtg actttttgcg tttctgaagc gtaagccgtt
     5641 tctacacaaa caaaggtctt gtagccatca tccggcatat cgcccatgct aactgaaagc
     5701 gccggtcctg ggttccagcc tacaacgttc agatgatgct ggtggcctac ggcgataata
     5761 cgattcagcg cttcgtcatt aattacgctg caatcttgcg gattcagata cacgcgatcg
     5821 gtacggtcag ggaaggtctg aataccgtcg gtcagtacat cttctttcgc gtcatttact
     5881 ttatcaatga agcgatcgcc tagtccactg acgcttacct tagcgatatc acccacgtta
     5941 aagtaggtat gcagagcaga ggtagtttca aattcgccgt gtgcttcaag atcgatttca
     6001 caggttttac ccacgtggaa atgcgctaac agcgtaaagt cgtgcggcca gaattttttc
     6061 gtctcttcgc tttgtgtcaa ttcaaaagtc agcgctacgc catcagcatc ttcacgatgt
     6121 gatttcagcg cccacggcag gttgcgggca aaaccgtgcg caggcagacc ttgttgtgca
     6181 gccggaccaa accacggcca gcaaaccggt atgccaccgc gaatggcgac gccatttttg
     6241 aacggtgtgt tgttactcaa ccacagaact tcttcttcac ccgcaggttt ccacgagaga
     6301 aggtgtgcgc cctgtaatgc aaaagaggct tttacctggg gatgatcgac cacaatgagg
     6361 tccagttcat ccagtttacg acgggagagg acaggggaga tttgttcgat gaccggaagg
     6421 gcaaaaattt tcttaatcat gacgcagtcc tttaacttca ttttatcagg taaaaaaaag
     6481 agcgaccgaa gtcgctcttt ttagatcaca gagtcatctc aacttatttg gagatgtgag
     6541 cgatcaggtc cagaactttg ttggagtaac cggtttcgtt gtcgtaccag gataccagtt
     6601 tcacgaagtt gtcgttcaga gcgatacccg ctttagcatc gaacacggaa gtgcaaactt
     6661 cgccgttgaa atcggtagat actacgtcat cttcggtgta gcccagaacg cctttcattt
     6721 cgccttcagc agcagcttta acggcagctt tgatctgctc gtaagttgca gctttttcca
     6781 gacgaacggt caggtcaact acggatacgt tcggggtcgg aacgcggaac gccataccag
     6841 tcagtttgcc attcagttct ggcagtactt tacctacagc tttagcagca ccggtagaag
     6901 acgggatgat gttctgggaa gcgccgcggc cgccgcgcca gtctttgtga gacgggccat
     6961 caacggtttt ctgagtagcg gtagtagcgt gaacggtggt catcagacct tcgatgatgc
     7021 cgaagttatc gttgataact ttagccagcg gagccaggca gttggtggtg caggaagcgt
     7081 tggaaacgat gtcctggcca gcatatttgt cgaagttagc gcctttaacg aacatcggag
     7141 tgttgtcttt agacggacca gtcataacca ctttcttcgc accagcggtg atgtgtttac
     7201 gagcagtttc gtcagtcagg aacagaccag ttgcttcagc gacaacgtca acaccaactt
     7261 cgtcccattt caggttagcc ggatcacgtt cagcggtaac acggattttt ttaccgttaa
     7321 cgatcagatg accgtctttc acttcaacgg taccgtcgaa acggccgtga gtggagtcat
     7381 atttcagcat gtatgccatg taatcagcgt ctaacaggtc gttgattgca acgatctcga
     7441 tgtcagaacg tttctgagca gcacggaaaa caatgcgacc gatacggcca aaaccgttga
     7501 tacctacttt gatagtcata tattccacca gctatttgtt agtgaataaa aggttgcctg
     7561 taaaattaca aaaaccttac gcagcgtcaa gcggaatcgt gtcaatcatt gcgacaaatc
     7621 aatcctgtgc ctaagcatta cgcgactgac tcgcctcact cttcctttgg gcttgagacc
     7681 acatggggtc ggcgccccga attttaaagg gcaattacga taaaaatgtg attttcatca
     7741 cgatttaggt gcagccattt cgtcgagatc aagtttagga caaaattttg ccagttggtg
     7801 ttaatgtttt gttagaatca gtcacgtaat gtgagcacga ttaaagtgag atgtgagcaa
     7861 atggctaata aaccttcggc agaagaactg aaaaaaaatt tgtccgagat gcagttctac
     7921 gtgacgcaga atcatgggac agaaccgcca tttacgggtc gtttactgca taacaagcgt
     7981 gacggcgtat atcactgttt gatctgcgat gccccgctgt ttcattccca aaccaagtat
     8041 gattccggct gtggctggcc cagcttctac gaaccgttga gtgaagaatc tattcgttat
     8101 atcaaagact tgtcgcatgg aatgcagcgc atagaaattc gttgcggtaa ctgcgatgcc
     8161 catctgggcc atgtctttcc tgacgggccg cagccaacgg gcgaacgtta ttgtgttaac
     8221 tctgcttctt tacgcttcac tgatggcgaa aacggcgaag aaatcaacgg ttgaaaaaac
     8281 gattcagcaa cttattccac aggagcggaa aaattatgaa tcttgatgac attatcaaca
     8341 gcatgacgcc tgaagtatac cagcgtttgt cgaccgccgt tgaactgggg aaatggcctg
     8401 atggcgttac gttgaccgag gaacaaaaag agaactgcct gcaactggtg atgctgtggc
     8461 aagcccgcca taataccgaa gcacagcata tgacaattga cacaaatggt cagatggtca
     8521 tgaagagcaa acagcagtta aaagaagatt ttggtatctc agcaaagcct attgccatgt
     8581 ttaagtaagt ggtttatccc ctttttgccg cggttataaa ggggatagcc aacaatgacc
     8641 ccacgactca tctcaattat ctcgccccga cctggctaag tcccagtccg atctcttgca
     8701 gtcgcgttct cacctgaatg acttctcctg aacttaagtc tcaggcctat ttggccggta
     8761 atccctctcg aatttgctct ttttgcgtcc cgccttccat caacctgtaa cagccccagc
     8821 cattttctgc gtaattgtga tctaagttgt aaaacgcaga tcattatctg tgttttacag
     8881 atttaatgtt gacctcaaaa atcatgtggg taagtatgat gaccatctaa gcaaaacaaa
     8941 acacagagga tgacgcaatg aaagcactgg ctcggtttgg caaggccttt ggcggctaca
     9001 agatgattga tgtcccacaa cccatttgtg gcccggaaga tgtcgtgatt gaaattaaag
     9061 ccgcggcaat ctgcggcgca gacatgaagc actacaatgt cgatagcggt tcggatgagt
     9121 ttaactctat ccgcggccat gagttcgcag gttgtattgc gcaggttggt gaaaaagtca
     9181 aagactggaa agtggggcaa cgcgtcgtgt cggataacag cggtcacgtt tgcggcgttt
     9241 gcccggcctg tgaacaaggt gattttctgt gttgtacaga aaaagtaaac cttggtctgg
     9301 ataacaatac ctggggcggt ggtttttcca aatattgtct tgttcctggt gaaattctca
     9361 aaattcatcg tcattcgttg tgggaaatcc ctgatggtgt tgattatgag gacgcagccg
     9421 tacttgaccc tatctgtaat gcctacaaat ccatcgcgca gcaatcgaaa ttcctccctg
     9481 gtcaggatgt ggtcgtcatc ggcactggcc cactcgggct gttctccgta caaatggcgc
     9541 ggattatggg ggcggtaaat atcgtcgtcg ttggtctaca agaagatgtg gcggtccgct
     9601 tcccggttgc aaaagaactg ggtgcgacgg cagtagtaaa tggttctacc gaagatgtgg
     9661 tggcgcgctg ccagcaaatt tgtggcaaag acaatctggg actggtgatt gaatgctccg
     9721 gtgccaatat cgcactgaaa caagccatcg aaatgctccg tccgaacggg gaagtggtac
     9781 gcgttggaat gggcttcaaa cctcttgatt tctcgattaa tgacattacc gcctggaaca
     9841 aaagcatcat tgggcatatg gcctatgact ccacctcatg gcgtaacgct atcaggctat
     9901 tagccagcgg cgcgatcaaa gtcaaaccga tgatcacgca tcgtatcggc ctgtcgcaat
     9961 ggcgcgaagg gtttgatgcg atggtcgata aaactgcaat caaagtgatc atgacttacg
    10021 actttgatga ataactactc tcctgaagga acgttatcgc atggaacaga taacaaaacc
    10081 gcattgtggt gcacggctgg atcgcttacc ggattgccgc tggcattcgt caatgtttgc
    10141 catcgtcgcg tttggcttgc ttgtctgctg gagtaatgcc gttggtggct tgatcctcgc
    10201 gcagctgaaa gcgttgggct ggacagataa ttccaccact gccacattct cagcaatcac
    10261 gaccgccgga atgtttctcg gtgcgctggt gggcggcatc atcggtgaca aaaccggtcg
    10321 cagaaatgcg tttatcctct atgaggccat tcatattgct tcgatggttg tcggtgcttt
    10381 ctcaccgaat atggatttcc ttattgcctg ccgttttgtg atgggcgttg ggctgggagc
    10441 tttactggtt acactgtttg ctggtttcac cgaatatatg cccggaagaa atcgcggaac
    10501 gtggtccagt cgggtttcct ttattggtaa ctggtcatat ccgctctgtt cattgattgc
    10561 gatgggactc acgccgctga ttagtgcaga gtggaactgg cgggtacaac tgcttatccc
    10621 tgcaatattg tcacttatcg ctacggcgct ggcctggcgc tactttcctg aatccccgcg
    10681 ctggctggaa tcgcgcggac ggtatcagga agccgagaaa gtcatgcgga gtatagaaga
    10741 aggcgtcata cgccagacgg gtaaaccttt gccgcctgtg gttattgctg atgacggtaa
    10801 agcaccacaa gcggtgccgt attcagcctt actgacagga gtattactga aacgcgtgat
    10861 attaggctct tgtgtgctga ttgccatgaa cgttgtgcag tacacactaa ttaactggtt
    10921 gccaacgata ttcatgaccc agggaattaa tttaaaagac tcgattgttt taaataccat
    10981 gagtatgttt ggcgcgccat ttggtatttt tattgccatg ctggtgatgg ataaaattcc
    11041 gcgtaaaaca atgggggtgg ggctattaat cctgattgcg gtgctcggat atatctattc
    11101 actgcaaacc agtatgttgc tcataacgct gattggtttc ttcctgatta ctttcgtcta
    11161 tatgtacgtt tgctatgcct cggcagtgta tgtccctgag atctggccga cagaggccaa
    11221 actccgtggc tccggtctgg cgaatgcggt agggcgaatc agtggtattg ccgcgcctta
    11281 tgccgttgca gtgctgctca gtagttatgg cgtaacggga gtctttattc ttctgggggc
    11341 tgtttcaatt attgtcgcaa ttgctatcgc caccattgga attgaaacta aaggtgtctc
    11401 cgttgaaagt ttaagtattg atgcagtagc caataaataa tactggtgga ttcaaaatga
    11461 aaaattcaaa agcaatattg caggtgccgg gcacaatgaa aattatttca gcagaaatac
    11521 ctgtgcctaa agaagatgaa gttttgatta aagtggaata tgtcggtatt tgtggttcag
    11581 atgtacatgg ttttgaatca ggcccgttta ttcctcctaa agacccaaat caagaaattg
    11641 gcctgggtca tgaatgcgcc gggacggttg tggctgtggg aagccgcgtg cgcaaattta
    11701 aaccggggga tcgggtaaat atcgaacctg gcgttccttg cggtcactgt cgttactgtc
    11761 tggaaggcaa atataacatt tgcccggacg ttgattttat ggcgacacaa cccaactacc
    11821 gcggcgcatt aacgcactat ctgtgtcatc cggagagctt tacttacaaa ctgccagaca
    11881 atatggacac gatggaaggg gcgctggtgg agcctgccgc agtcgggatg catgccgcga
    11941 tgctggcaga tgttaaacct ggtaagaaga taattattct gggagcgggt tgtattggtt
    12001 tgatgacgtt gcaagcgtgc aaatgcctgg gagcaacgga aattgccgtc gttgatgtgc
    12061 tggaaaaacg tctgacaatg gcggaacagc tcggcgcgac agtggttatt aacggcgcaa
    12121 aagaagacac tattgcacgc tgtcagcaat ttaccgaaga catgggcgca gatattgttt
    12181 ttgaaacagc gggttctgcg gtcaccgtta aacaggcacc ttatctggtc atgcgcggcg
    12241 gtaaaattat gattgttggt actgtacccg gcgattcggc aatcaatttc ctcaaaatca
    12301 atcgcgaagt cactatccag acggtattcc gctatgccaa tcgttatccg gtcacgattg
    12361 aagctatttc ttcagggcga ttcgatgtga aatcgatggt gacgcatatt tacgattatc
    12421 gggatgtaca acaggcattt gaagagtcag ttaacaacaa acgcgacatt attaaaggcg
    12481 ttattaaaat tagcgattaa ttaatacggg agaacagaac atgctcgcag atatcagata
    12541 ttgggaaaac gatgccacaa ataaacatta tgcaattgcc cattttaacg tatggaatgc
    12601 agaaatgttg atgggcgtta tcgacgcagc cgaagaagcg aaatcaccgg ttattatttc
    12661 ttttggcaca ggttttgtcg gcaacacctc atttgaagat ttctctcaca tgatggtatc
    12721 aatggcacaa aaagcaacgg tgccggtaat aactcattgg gatcatggtc ggagtatgga
    12781 gattattcat aacgcctgga ctcatggtat gaattcatta atgcgtgatg cttctgcatt
    12841 tgatttcgaa gaaaatattc gtttaaccaa agaggctgtt gatttcttcc atccgctggg
    12901 tattccggta gaggcggaat tagggcatgt cggtaatgaa accgtttatg aggaagcgct
    12961 ggcaggctat cactataccg atcctgacca ggcggctgaa tttgttgaac gtacgggctg
    13021 tgactcattg gctgtcgcca tcggcaacca gcatggggtt tatacgtcag agccacaatt
    13081 gaactttgag gtcgtcaaac gcgtacgcga tgctgtttct gttccgctgg ttttgcatgg
    13141 cgcatcaggg atcagtgatg ccgacattaa aactgcaatt tcgttgggta ttgcgaaaat
    13201 caacattcat acggagctct gtcaggccgc aatggtagca gtgaaagaaa accaggatca
    13261 gcctttcctg catttagaac gtgaagtgcg taaagctata aaagaacggg cattggataa
    13321 aattaaacta ttcggttctg atggcaaagc ggaataataa tatggataat ctcgacgtta
    13381 tttgtatagg tgccgctatt gttgatattc cattgcaacc ggtcagtaaa aatatctttg
    13441 atgtggattc ttatcctctt gaaagaatcg caatgaccac cgggggggat gcaataaatg
    13501 aagcaacaat tatttctcgt ctgggccatc gcacagcatt aatgagtcgt attggtaaag
    13561 atgccgcagg gcaatttatt ctcgaccatt gccgcaaaga gaatattgat attcaaagcc
    13621 tgaagcagga cgtcagcata gatacctcta ttaacgttgg actggtgacg gaggatggtg
    13681 agcggacctt tgtcacaaac cgtaacggca gtctgtggaa attgaatatt gacgatgttg
    13741 attttgcgcg gttttctcag gcgaaattat tatcactggc cagtattttc aacagtcctc
    13801 tattggatgg tagagcgcta acagaaattt ttacgcaagc taaagcccgg cagatgatta
    13861 tctgtgccga tatgatcaaa ccgcggttga atgaaacgct gaatgatatt tgcgaagcat
    13921 tgagttatgt cgattatctg tttcctaatt ttgccgaggc aaaattactc accgggaaag
    13981 agacactgga tgaaattgct gactgctttc ttgcgtgcgg cgtaaaaacg gtggtgatta
    14041 aaacgggtaa agacggctgc tttatcaagc gtggtgacat gacgatgaag gtgccggcgg
    14101 tcgcaggaat aaccgctatc gacaccattg gtgcgggcga taactttgct tcaggtttta
    14161 ttgcagcact gttagaaggt aaaaatctgc gtgaatgcgc acgctttgcc aatgcaacgg
    14221 cggctatctc ggttctaagc atcggcgcca ccactggtgt aaaaaacaga aagctggtcg
    14281 aacaattgct ggaagaatac gaaggataat gaaggcaaat gaaaaagata cctttaggca
    14341 caacggatat aacgctttcg cgaatggggt tggggacatg ggccattggc ggcggtcctg
    14401 catggaatgg cgatctcgat cgacaaatat gcattgatac gattcttgaa gcccatcgtt
    14461 gcggcattaa cctgattgat actgcgccag gatataactt tggcaatagt gaagttatcg
    14521 ttggtcaggc gttaaaaaaa ctgccccgtg aacaggttgt agtagaaacc aaatgcggca
    14581 ttgtctggga acgaaaaggg agtttattca acaaagttgg cgaacggcag ttgtataaaa
    14641 acctttctcc ggaatctatc cgcgaagagg tagaagccag cttgcaacgt ctgggtattg
    14701 attacatcga tatctacatg acgcactggc agtcggtgcc gccatttttt acgccgatag
    14761 ctgaaactgt cgcagtgctt aatgcgttaa aggccgaagg gaaaattcgc gctataggcg
    14821 ctgctaacgt cgatgctgac catatccgcg agtatctgca acatggtgaa ctggatatta
    14881 ttcaggcgaa atacagtatc ctcgaccggg caatggaaaa cgaactgctg ccgctatgtc
    14941 gtgataatgg cattgtggta caggtctact ctccgctaga gcagggattg ttgaccggga
    15001 ccatcacgcg tgattacgtt ccgggcggcg ctcgggcaaa taaagtctgg ttccagcgtg
    15061 aaaacatgct gaaagtgatt gatatgcttg aacagtggca gccactctgt gctcgttatc
    15121 agtgcacaat tcccactctg gcactggcgt ggatattaaa acagagtgat ttaatctcca
    15181 ttcttagtgg ggctactgcg ccggaacagg tacgtgaaaa tgtcgcggca ctgaatatca
    15241 acttatcgga tgcagacgca acattgatga gggaaatggc agaggccctg gagcgttaaa
    15301 tactcaacac gattcacctc tgtttctgtg aaaactgtga tgaaaatctg tgattcaaac
    15361 aggttatttt gaaagtaaac atcggttatg cgataattgc gctaatgtga tgtaaaaaga
    15421 tacaggggtg taatcgtggc ggcaaaagac aggattcagg caattaagca aatggttgcc
    15481 aacgataaaa aggtgacagt ctcaaatttg agtgggattt ttcaggtaac cgaagaaacc
    15541 attcgccgcg atcttgagaa gttggaagat gaaggctttt tgaccagaac ctatggtggt
    15601 gctgttttaa atacagcgat gttgacggag aatatccatt tttataagcg cgcttcatcg
    15661 ttttatgaag agaagcagct tattgcacgc aaggcactac cctttatcga caataaaacc
    15721 accatggcag ccgattccag tagtaccgta atggaattgc tcaaattgtt acaggaccgt
    15781 agtgacctga cgttgctaac caactcggcg gaagcgattc atgttctggc tcagtcagaa
    15841 attaaagtcg tttcaacggg tggggaacta aacaaaaata cactttcact gcaaggaaga
    15901 attactaaag agatcatcag ccgctaccat gtcgatatca tggtaatgag ttgtaaaggt
    15961 cttgatatta acagcggcgc gctggactct aatgaagcgg aagctgaaat caaaaagaca
    16021 atgatccgtc aggcgacaga agttgcgtta ttggttgatc actctaagtt tgatcgcaaa
    16081 gcttttgtcc agttaactga ctttagtcat attaattaca taataactga taaatcaccg
    16141 ggtgcagagt ggatagcatt ttgcaaagac aataatatcc agctggtgtg gtaatttaag
    16201 cacacattgt caatattatt tgttcctgcg cctggaaata aaatgatgat aaagatgcgg
    16261 caagtgaatt tgccgcaatt gctacctttt ttagcaaatc cgaggcaccc aatggaacaa
    16321 tatgatcaaa ttggcgcaag actggaccgc ttgcctttgg cccggtttca ttatcgtata
    16381 tttggtataa taagctttag tctgttatta acggggtttt tgagttactc cggtaatgtc
    16441 gtcttagcaa agctggtaag caatggatgg tcaaataatt tcctcaatgc cgcctttacc
    16501 tcggcattaa tgtttggtta tttcatcggc tctcttaccg gtgggtttat tggtgactac
    16561 tttgggcggc gcagggcgtt tcgcataaat cttctcatcg tcggtattgc ggcaacaggg
    16621 gccgcttttg tccctgatat gtactggctc atcttctttc gcttcctgat gggaacagga
    16681 atgggggcgc tgattatggt tggctacgcc tcatttacgg agtttatccc cgcgacggtg
    16741 cgtggaaaat ggtccgcgcg gctctcattt gttggtaact ggtcgcccat gctctctgcg
    16801 gcgataggcg tggtggttat cgcttttttt agttggcgga taatgtttct gctgggcggt
    16861 attggcatac tgttagcgtg gtttctctca ggtaaatact ttattgagtc gccacgatgg
    16921 ctggcaggga aagggcaaat cgcaggtgca gaaagccaac ttcgtgaagt agagcagcaa
    16981 attgaaagag agaagaggat tcgtttaccc ccgcttactt tgaaccagag caacagcaag
    17041 gttaaggtaa tcaagggtac cttctggctc ctgtttaaag gggaaatgtt acgacgtaca
    17101 ttagtcgcga ttactgtttt aattgcaatg aacatttcgc tttataccat caccgtatgg
    17161 ataccgacca tatttgttaa ctccggcatt gatgttgata aatcaatatt aatgaccgct
    17221 gttattatga ttggcgctcc ggtagggata tttattgcgg cattaattat tgatcatttt
    17281 cctcgtcgat tatttggctc tgccttactt attattattg ctgtgttagg ctatatctat
    17341 tcaattcaga ctacagagtg ggcgatttta atctatggcc tggtgatgat cttcttttta
    17401 tacatgtatg tgtgcttcgc gtcggcggtt tatatcccgg aactttggcc aacgcattta
    17461 cgcctgcgtg gttcgggttt cgttaatgcc gtcggacgga tcgtcgcagt cttcacgccc
    17521 tatggcgttg cggcattatt aacacattat gggtcgatca cggtgtttat ggtgcttggt
    17581 gtcatgttag tgctctgtgc gctggttctc tccatttttg gcatcgaaac gcggaaggtg
    17641 tcgctggaag agatttctga ggtgaattaa atctttcgtt gatggcgtca attaccagat
    17701 gtatcactga tgtgttttat ctggcctaca atgccagtac agaattgtag gccagcgtaa
    17761 aattactcct gcgtttcttc ccagtctgcc agcgtatata gcgttgcccc agctgctgac
    17821 atctccataa acgcgtgcgc actgtcctgt ggctggatat tcacgccacg acaaccatcg
    17881 gtaatcacgt tcaccttata acctaactgt aacgcgtcca gcacggtaaa cttaacgcaa
    17941 tagtcagtag ccagacccat aacgatcaat tcattgatta catgagcgcg caaccagtca
    18001 tcgagcgcag ttttctgccg acggccgtta tcaaaaaagg cactgtaact gtcaactaaa
    18061 ggattttcgc ctttatggaa caccgctgcg atcgcttttt gcttcagtaa cggatgtaat
    18121 tgtgcgcctt cactgttctg cacacagtga tcgggccaga aggtttgtgg caaaccgtcg
    18181 agttggcccg gcgtataagg ctctacaccg tgctgactgg caaaactgcc gtgattcgcc
    18241 gggtgccagt cctgactggc gataaccgct tcaccgcgcg actggcacca gtcaatcagg
    18301 cggttagcga catccaccgt actgtcacct tccggcacgg cgagcgcgcc accagcacag
    18361 aaatcatttt gtaaatcgac cagtaacagg gcgcgagggg gcattgcagt ctccttaatc
    18421 atccggcgtc agttcgccac gcaggttttg gctcatggcc ttgcgaatgg tttcagtatc
    18481 aagttcctgg ctaagcaggt aatgcagttt ggttagcgtg gcttctacag tcatatctgc
    18541 accgccaata acgccggcat gggcgagggc gttaccagtg gcataaccac ccatgttcac
    18601 tttaccggac atacattgtg tcaggttgac caccacaata ccgcgatcgc tggcttcttg
    18661 taattcctgc aggaaggctt tgttttgtgg cgcattaccc acgccatatg agcgcagaat
    18721 caatgctttc acaggttggc gcagaaaatt gcgcacgacg tcagcagaaa tccccggata
    18781 aatcgtcact acgccaattg gttgcggcgt gattggatga acgatcaatg ccccttcacc
    18841 gtgcgggacg ggtggcgtat tcaaacgacg aatatggata cctgcttcca gtaacggagg
    18901 aaggtttgga gaggcaaacg catcaaaacc atcggcatgg gctttggtgg ttctgttgcc
    18961 acgatacagt cgattattaa aaaagagagt tacttcgttg ataggataat tcgccgcaac
    19021 gtacaacgca ttcagcagat taatttgtcc gtcagagcgt aactcagcca gcgggatttg
    19081 tgaccctgtc acaatgaccg gtttaccgag attctcgagc atgaacgaca gtgcagaggc
    19141 ggtatacgcc atcgtgtctg taccgtgcag aatgacaaaa ccatcataat cgtcatagtg
    19201 cgctttaata tcttcagcaa tatgctgcca gtcttctggc gtcatatcag aagaatccat
    19261 caatggcgta tattcatgaa tggtgaaatc tggcatctcc gggcgatgga attccggcat
    19321 cagcgccagt tggcgctgta gatgacctga caccggaata taaccctgct cggaacgctg
    19381 catcccgatg gtcccgcccg tgtaggcgac gtaaattgat ttcttttgca tgatattgat
    19441 atgaatgata tcgaaagagc aaaagtatat acgtgaggca gggggagcgc acctgatact
    19501 atgaggataa ggaaatatgt ggcgagacag cgggaagagg attcgtcgac gtaaaaagta
    19561 atgtggccga cgatctgtcg gccacttagt acaagactta acgcacgttg gcgcaggtca
    19621 ggcaaaacgc ataacggttt tgtgggtcgt taaacgcggc tagcttgtcg ctttcacttt
    19681 taacggtaga ggctaccgag gccagcggtg caggtaacat ggcctggaac gcatctggca
    19741 acattgcccg gacagaacca gacatgttgt ccatcacttt gtcgaagaag gtcggttcat
    19801 caacgtagta ttccagatgc cactgtttca cttttgccag ctctgctgct ttggcgaccg
    19861 catcatcgaa atccccgaga ctatcgacca gcccgttagc ttttgcatcc tgaccggtcc
    19921 agacgtgacc ctgggcgatt ttatcaatct gctccggcgt tgaatgacgc gcatcagcaa
    19981 ccagcgtgat aaagcgttta tagccattct caatgcttaa ctgcatcatc tgctgcgctt
    20041 ccggcggcag tgccttggtg atagaaacat ctgccagcgg tgaagttgag acaccatcag
    20101 tatgaacacc aatcgaatcc agactatttt ctacggtggt gatcacgccg aagataccga
    20161 tagaaccggt cagggtgctg gggttagcca caatgtaatt agctggtgtg gaaatccagt
    20221 aaccaccaga tgccgccatg ccgcccatcg atacaaccac aggcttaccc gctgcccggg
    20281 ctgctgccag ttcagcgcga atcacttcag acgcggtaac gctgccgccg ggactattaa
    20341 cacgcagaac aatcgctttc actttcgggt caaggcgagc gtcgcggatt tgtgccgcag
    20401 tggtatcacc gccaacattc ccctgagttt cctcgccatc cataattgcg ccattagcga
    20461 agacgacacc gatactgtca ccggtatctg ccggcgtttt caatgcgtaa tcgtaataac
    20521 tgatggcgcg ataattttta tcagttttac tccagccgaa ctctttggtc agagtttttt
    20581 cgatttcggc actcgatgcc agtgcatcga ccagcttgtt ttccagcgca tatttcgcgg
    20641 tatcgccacc ggttttggtt aaaccctcaa gcaacccttg cgcgccaggg aatacctgct
    20701 gagcagggat ctggcggtta gcggcaacag tattcagata gttttgccac agctcaccaa
    20761 tccagcggct gtcagcttcg cgggctgccg gcgacatatc atcacgaata aacggttcaa
    20821 cggcagattt atacgtaccc acgcggaaca catgggtgga aactttcagc ttatccagta
    20881 acgatttgta gtacagaccg ttggtggcaa agccgtgcag atcaaccacg ccttgcggag
    20941 acagccaaat tttattggcg aaactggcga gataatattg cccctggcta tagttctcgc
    21001 caacggcata aaccggtttc ccgctatcac gaaactcttt cagagctttg ccgatgtact
    21061 gcatagacgg ctggtcgccg cctgcgaagt ttttcagatc catcacaata ccggtgatat
    21121 tgcggtcgtc cttcgcctgg cgaatggtgt tgacgatatc aaacagtgag ttttcctgca
    21181 gacgatcgga actggcacca agcagctggc ggcttaattt actaaaccgc tgagaactgt
    21241 cgggtttatc gacgatcaca ccagaaatgt ccagcagcag tgcgccacga ctggccgttt
    21301 ctttcgaatc accaccactg acctgcatcc agatccccac accaaccagc acgagaaaaa
    21361 taaagaacag gttaagtacc atttcacgga cgaaattcag cagacgccac gtccatttaa
    21421 aaaatccggc aataaatcgc caaagggttc gcatgtattc tcccaactta aggtcacacc
    21481 tgtttcaggc gcaatagatg tggttatcgt aatgacccaa ccggcacttg tcagcaggaa
    21541 ttgcggactg ggctgtaaca aaatccactc tcgtgttaat tttgtgaata aatatcatga
    21601 caggagttaa tcaaatggat gcactcgaac tattgatcaa tcgccgtagc gcctcccgcc
    21661 tggctgaacc cgcgccaacg ggtgaacaac tgcaaaacat cctgcgtgcg ggtatgcgtg
    21721 cgccggacca taagtccatg caaccgtggc atttctttgt gattgaaggt gaagggcgcg
    21781 agcgtttcag cgctgtactg gagcaggggg cgattgctgt cggtagtgat aacaaagcaa
    21841 tcgacaaagc gcgtaatgcg ccgttccgcg caccgctcat cataacggtg gtggcaaaat
    21901 gcgaagagaa tcataaagtc ccgcgctggg aacaggaaat gtctgcggga tgcgcggtca
    21961 tggcgatgca aatggcagca gttgcccagg ggtttggcgg catctggcgc agtggcgcat
    22021 taactgaaag tccggtagtg cgtgaagcat tcggttgccg tgagcaggat aaaattgtcg
    22081 gttttctcta cctcggtacg ccgcagttga aagcatctac gtcgataaac gtcccggacc
    22141 cgacgccgtt tgtgacttat ttctgataat tgtcgcgaaa ctgtctggat tgtgagcaaa
    22201 ggcgcaggaa ttcagacact ctcacttatc actttatgga atgagggtta ccatacccgg
    22261 attgcattga caggagatgt ccatgagcga gaactcgatt cgtttgaccc aatacagcca
    22321 cggcgctggt tgcggctgta aaatttcccc aaaagtgttg gaaaccatcc tgcacagtga
    22381 gcaggcgaag tttgttgatc cgaatttgct tgtgggtaat gaaacccgcg acgatgcggc
    22441 ggtatacgat ctgggcaatg gcaccagcgt tatcagtacc accgacttct ttatgccgat
    22501 cgttgataat cctttcgatt ttggccgcat tgcggcgact aacgccatca gcgatatctt
    22561 tgctatgggc ggcaaaccga ttatggcgat tgcgatcctc ggctggccga ttaacaaact
    22621 ttccccggaa attgcccgcg aagtgaccga aggtggacgc tatgcatgcc gtcaggcggg
    22681 tattgcgctg gctggcggtc actccatcga tgcgccggag ccgatttttg gtctggcggt
    22741 aacggggatc gtaccgaccg agcgggtgaa gaaaaacagt accgcacaag ccggatgcaa
    22801 actgttcctg acgaaaccgc tggggattgg cgttcttacc acggctgaga aaaaatcact
    22861 gctgaaacca gaacatcagg gactggcgac ggaagtgatg tgccggatga acatcgcagg
    22921 cgcgtccttt gccaacatcg aaggcgtaaa agcgatgacc gacgttacgg gttttggttt
    22981 gctggggcat ttaagcgaaa tgtgtcaggg cgcaggtgtg caggcgcgcg tcgactatga
    23041 cgcgatcccg aaattgccgg gggttgaaga gtacattaag ttgggcgcag tgcctggcgg
    23101 caccgaacgt aactttgcca gctacggtca tctgatgggt gaaatgccgc gtgaagtgcg
    23161 cgatctgctg tgcgatccgc aaacttctgg cggtttgctg ctggcggtca tgccggaagc
    23221 agaaaatgag gtcaaagcta cagccgccga gtttggcatt gaactgacgg cgattggcga
    23281 actggtgcca gcgcgcggcg gtcgtgccat ggttgagatc cgttaattca atgcggttgt
    23341 ttattgccga aaaaccgagt ctggcgcgcg ccattgctga tgtcctgcct aaaccgcacc
    23401 ggaaaggcga tggctttatc gagtgcggta atggtcaggt ggtgacctgg tgtatcggtc
    23461 acctgcttga gcaggcgcag ccagacgcct acgacagccg ctatgcgcgc tggaatcttg
    23521 cggatttgcc gattgtcccg gaaaagtggc aattacagcc ccgaccctcc gtgactaaac
    23581 aacttaatgt catcaaacgc ttcctgcatg aagccagcga aatcgttcac gccggagacc
    23641 cggatcgcga agggcaactg ctggtggatg aagtgctgga ctacctgcaa ctggcaccgg
    23701 aaaagcgcca gcaggtacag cgttgtttga taaacgacct gaacccgcag gcggttgagc
    23761 gggcgatcga tcgtcttcgc tccaatagcg agtttgtacc gctgtgcgtt tctgcactgg
    23821 cgcgggcgcg ggctgactgg ctgtacggca ttaatatgac ccgtgcgtat accattctcg
    23881 gtcgcaatgc cggttatcag ggcgtgcttt ccgtgggacg cgtgcagacg cccgtactcg
    23941 gactggtggt gcgccgcgac gaagagattg aaaacttcgt ggcgaaagac ttctttgaag
    24001 tcaaagcaca tatcgtgaca cctgccgatg agcggtttac cgctatctgg caaccgagcg
    24061 aagcgtgtga accgtaccag gatgaagaag ggcgcttgtt acatcgtcca ctggcggagc
    24121 atgtggttaa ccgcattagt ggtcaaccag ctattgtcac cagctataac gataaacggg
    24181 aatcagaatc cgcgccgctg cctttttcgc tttcggcgtt gcagattgaa gcggctaaac
    24241 gttttggtct gagcgcgcag aacgtgcttg atatctgcca gaagctgtac gaaacgcaca
    24301 agctaatcac ttatccgcgt tccgattgcc gctatttgcc agaagaacat tttgccggac
    24361 gccacgcggt gatgaatgct atcagcgttc atgcaccgga tctgttgccg cagccagtgg
    24421 tagatccaga tatacgcaac cgctgttggg atgacaaaaa ggtcgatgcg caccacgcca
    24481 tcattccgac cgcacggagt tctgcgatca acctgacgga gaacgaagcg aaggtctata
    24541 acctgattgc ccgtcagtac ttgatgcagt tctgcccgga tgcggtgttc cgcaagtgtg
    24601 ttatcgaact ggacattgcc aaaggcaaat ttgtcgctaa agcccgtttt cttgctgaag
    24661 caggctggcg cacgctgtta ggcagcaaag agcgcgatga agaaaacgac ggcacgccgc
    24721 tgcctgtggt ggcgaaaggc gatgaattgt tgtgcgaaaa aggcgaagtg gtagagcggc
    24781 aaacccagcc gccgcgccat tttaccgatg caacactgct ttcggcgatg accgggatcg
    24841 cgcgctttgt gcaggacaaa gatctgaaaa agatccttcg tgcgaccgat ggtttgggga
    24901 cagaagcaac gcgtgccggg attattgaat tgttgttcaa gcggggtttc ctgaccaaaa
    24961 aagggcgcta tatccactcc accgacgccg gaaaagcgct attccattcg ctgccagaaa
    25021 tggcgacgcg accggacatg accgcgcact gggaatcggt gctgacgcaa atcagcgaaa
    25081 agcagtgtcg ctatcaggac tttatgcagc cgctggtggg gacattgtac cagttgattg
    25141 atcaggcaaa gcgcacgtcg gtaaggcagt ttcggggaat aatggctccc ggcggtagag
    25201 aagggaagaa aaaggattcg ccacgcaaga gagcgccgaa aaaaagcccg ccatcagaag
    25261 aggcgggcaa tggagtgata acataaaggt tctttcaacc atctgatact gaagattttt
    25321 atggccggat aaggcgtttg cgctgtatcc ggcacaattg tcttaaatca cgccctggct
    25381 taacatcgca tccgcaacct tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt
    25441 ttgctcacct tcaccaccat gctcaacaca ggcatggtgg atatccagca tgatgtgatg
    25501 caaacgtgcg tcaactttct cggctttcca gcccaggcgc gcagcgtttt gcgccatttc
    25561 caggcccgat gttgctacac cgccagcatt agccgcttta cccggtgcaa acagtacgcc
    25621 tgcctgctgg aacagttcag tcgcttcgat ggtggtcggc atatttgccc cttcggcgac
    25681 ggctttaacg ccattggcga taagctgatg cgcggcgtca acatccagtt cgttctgggt
    25741 tgcgcaaggc agggcgatat caaccggtac agaccacggt tgttggcctt cgagatagac
    25801 cagaccaaat tctttggcgt aatctgccac tcgaccatcg cggctggctt tgatttcgat
    25861 aagacgcgcc agtttttctt tcgtgaatcc gctttcatca actacagtgc cgctggagtc
    25921 tgacgcagtg atcactcgag caccaaattc catcgctttt tcgatagcgt actgggcgac
    25981 gttgccggag ccagaaacgg aaacgcgcat tccttcaaaa cccataccgt ggcgttttag
    26041 cattgcttct gtgaaataaa ccagaccgta gccggtagct tccgggcgaa taagactgcc
    26101 gccaaatgaa aggcccttac cggtgaagac gcaggcggta ttgttggaga gctttttcat
    26161 catccccgcc ataaagccga cttcacgacc accaacgcct atatcacctg ccggaacgtc
    26221 agtatccgcg cccaggtggc gatacagttc agtcatcagc gcctggcaga aacgcataac
    26281 ttcgccttcg cttttccctt tcggatcaaa gtcgctgccg cctttaccac cacccatcgg
    26341 cagagttgtc agggcatttt taaaggtttg ctcgaagccg aggaatttga gaatggaaag
    26401 gtttactgac ggatggaagc gcataccgcc tttgtacggg ccgatggcag agctgaactg
    26461 cacacgccat gcacggttga cctgcacctg gttgcgatcg tcaacccata ccacgcgaaa
    26521 ctggatcacg cgctccggtt caaccagacg ctccagtaat gacatctgac gatatttggg
    26581 attttgttca agaaaaggcc agagtgtggt cattacttca cgaacggctt gcgcgaactc
    26641 ggtttgattc gggtcgcgct tttggacatg gttgaggaat gactccagag aatatgtctg
    26701 atccatagat ataaaaccct tatatattaa tacgattatg cttatgttgt ttatgtcatg
    26761 tgcttttgca gttttcgact ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac
    26821 ccaggaaagc aaattaataa cgagagtaat ctcataacca aatgcgataa ctcagacgct
    26881 aaagttttgc cgtccggcta ccgttatctg tagaagagac aataacgtta aggagttccc
    26941 tgtgagtcga gcattgatcg ccgtagtatt agcatttcca ttaattacac tggctaaccc
    27001 gcattatcat ccggacgtgg aggtaaacgt accgccagaa gtgtttagtt caggcgggca
    27061 gtcagcgcag ccctgtactc agtgctgcgt ttatcaggat caaaattatt ctgaaggggc
    27121 agtaattaaa gccgaaggaa tactgctgca atgccaacgg gatgacaaaa ccctaagcac
    27181 taatccgctg gtctggcggc gcgtgaagcc ataaacgcct ctaataatgg aatgtcagca
    27241 ggggccagcg gatatcgcag cgcctcttca ggtgagcacc agaccagcgc ctgatgttca
    27301 tgtgcctgta acgtcccgtg gaagtcgggt acgtgccagg catgaagatg gataatccgc
    27361 cccgaaactt ctcgctgatg gctggcaaca tagtcaccaa cagttgcttc gatgcccagt
    27421 tcttcgttta actcacgcac cagcgcctgc cgctggcttt catccggctc gactttacca
    27481 ccggcaaact cccataatcc cgcctgatcg ctatgggcag ggcgttgcgc gagtaaaatt
    27541 ttgccatcac gttcgatgat ggcggcaaca acttcaatca ttttcataaa ttacacacgg
    27601 gcagataagc gaaaaatgcc ataatatccg cactgttgct caatcagaaa ggctttatat
    27661 ttaaggagtg cctgtgctag accgccatct tcatccccgg attaaaccgt tgttgcatca
    27721 gtgcgtgcgg gttcttgata aaccgggcat tacgccagat ggtttaacat tagtgggatt
    27781 tgccatcggc gtgctggcac tgccttttct ggcgctgggc tggtatctgg cggcactagt
    27841 cgccattttg ttgaacaggc tgcttgatgg tctggacggt gcgctggcgc ggcgcagagg
    27901 gcttaccgac gcgggcggct ttctcgatat ttctctcgat tttctttttt acgcgctggt
    27961 gccttttggg tttatcctcg ccgcaccgga gcaaaacgcc ctggtaggcg gctggctgct
    28021 gtttgcgttt atcggcacgg gcagcagttt tctcgctttt gctgcgctgg cggcgaaaca
    28081 tcagattgat aatcctggtt atgcgcataa atcgttttat tatctgggcg ggttaaccga
    28141 aggcagcgag acgattttgc tgtttgttct gagttgcctc tttccggcct gttttccgtg
    28201 gtttgcgtgg gtatttggtg cgttgtgctg gatgacgacc tttacgcggg tgtggagtgg
    28261 gtatctgacg ctgaagtcgc tccagcgtca gtaattgcaa tgttatttac tgctgtccgg
    28321 gccgcgttca ccggttgcta ccggattttt tggatcgctg ctccattcgt accagccacc
    28381 gtcgtaaacg gagacgttat tccagcccat tgcgcgtgcg tacataaagg tttctgacgc
    28441 gcgccagcca gtgccgcagt agaatgaaac ttgctgatct ggtttgatat tccatgcttt
    28501 ccacatagcg gttatatcat cggcgctgcg catggtgcca tccgggttat ggaaatcttc
    28561 catatgcgtc gagtcgctac ctgcgtgtcc ccaacgtgct ccggctattt caccttttgg
    28621 tttaatatag ctgtaaccgc tggtcgtacc gataaattct ggccacgaac gaatgctcac
    28681 cagcgatgca tcctggcgat gcagcagtcc acgcgcttgt tccatatcaa gcatcagttg
    28741 cggttgtgcc gggatcttca cgccgaaatc cggttccgct ttcactttcg gtggcgttcc
    28801 gcgctcaacg ggcagacccg cgtcggacca ggtttgccag cctccgtcca gcaggcgcac
    28861 atctttcacg ccggcataaa gcataatttg cgccacacgc gctgcagcgt atacgtcacg
    28921 cccgtacaga atgaccgtgg tgtcatggcg aatgccgtgt tttgccagca tcgctttcag
    28981 ttgttcatca gaaactttgt tccacagcgg ttcgctttcc acttcgttgg tgtcgatgta
    29041 gtcagcgccg ggaatatggc tgataaggta aaattttgga gcgccccagg ccgcttcaat
    29101 gactttccag tcaccggcag gtttcgccgt aacctcttta ccttgttgca ggtcatgcag
    29161 ccactgcgga tataccagct gttcaaagtg cggcagtttt tgcagacggg aaggttcgct
    29221 tagcgcgtca ctcaggatgg agatatgcgt aaaacctgct ttttgcagtc gcgttttgac
    29281 ggcgtcgaca tctttgtcat taccgtacag cgccaccggg gcatcggctt tcaggttatg
    29341 ttgcttgatc cactcgttga gctgttcggt gctcattttg tcgagccagc tagcagagag
    29401 gtttaaggcg gcaggttcat gaccggaagg gccatttaag gtttgtggcc agccgttata
    29461 aaacgcgctg gggcgagtat caatcgcttt gccattttgt tgttgaagct ggtcaagtgt
    29521 aagaggcttc gccagttcag cggcccacga agaagcgcaa gccagcccta aagccattgc
    29581 cagcgcggtc atttgagaaa cacgtttcat cgaaaatccc gacattgtag ttttgagagc
    29641 gccattctga actgacgcat cgggaaaaac tttgcgttat cgcagtttgt tgtaattttc
    29701 tgaccactgc gccatatcca gaacggggct atcaggagga acatcctgaa gatcgtgcgt
    29761 cacctgcaca acagggatcg ccatggcgcg aatttcgctg aacacccaat ggcgaaaatt
    29821 atcgcgcagg gccacatcaa gacggctgaa gggctcatcc agtagcaacg cttttggctg
    29881 agcgagaagg gcgcgcagca gagcaacgcg cgctcgctga ccgccagaca aagtggcagg
    29941 atcctgatgg aacgctccgt caaggcctga acgctcaagt gcatcattca cggcattacg
    30001 tcgggcattc cccttaagtg tcgccggtag cgccagcagt aaattttgcc cgacgctgaa
    30061 ctggtcaaat aacagtgcat cctggaaaag aatgccaatc tgacgctgtg cggtgggtag
    30121 catgtcaatc cgttgctcat taagccatag ctcccctgta caagaaaact gtccggccag
    30181 cgcaccaatc atccacgaaa acagagtgga tttcccgcag ccggatggtc ccattaacgt
    30241 gacaatgtca cctttatcta ctgtaaagtt aacgtttgtc agcaagcggc tttctggtaa
    30301 acgtagcgaa acatttttca cgcagagcat tagcggagtc cttgtctgac ataacctacc
    30361 cattttgcga ctaacgcggt cagggcaaaa ataataagcg gtaacagcag ttgccataaa
    30421 gcctgggcgg cgagaatacc gttgctgccg ccgctgctta atgccaccgc ttcagtggtg
    30481 agcgtcggaa aacgccccgc gcccagccac agcgtcggca tatactgcgc aatactgact
    30541 gaaaatccca ccgcgaaggc aatcagcgca gggcgcaaca taagtggaca ttttacgtaa
    30601 aagaagattt tggcccgcga ccagcccagt gtttgcgcaa tcaaaattaa ccgtgaatca
    30661 atgcgctgcc aggcgggttg caggataaac agcatccacg gcattaccca cagcagatgc
    30721 ccccagacca ccgccgtcca gcttccatcc aggttcagcc atagcgccag cgtgtactgg
    30781 cccgccacca gaggcagagc gggtaataaa atgggcaacc atagccacaa ctggcgacgc
    30841 tgtggccccc attcctgcca cagcagtaac aggagcaaag cgatgaatgc cgccaccagc
    30901 cccattgtca ggctgttgat gagcgcttca ctattgatcg tcgactgatc cgcgaggatc
    30961 gccagcagaa ccacacacag cacaccggtt aagggtaaaa aactcgccag cgtattgcct
    31021 ggcaataaag gcgtggcagg cttacgaacg ccatctacgc ggggaatagt acgtcgccag
    31081 ccacgccata gcagatagcc cagcaaaacg taggcggcga gtaacagcat cagcagcagg
    31141 ctggcgagtg cacctttcgt ttgttgatcg gcgtcgccct gagttaacca ctgccagcta
    31201 attaccgcca gcgtcggggg attacctggc ccaagaataa tcgccacatc cacgaccgac
    31261 agcgaccagg cgacaatcgc cagcatcgcc attgccagcg cagacgcgac ggagggcaac
    31321 agcaaccagt tcaggcattg ccagcggctg tagcccagcg aatccagcac aatgagttgc
    31381 tgcaacagcc gtttttcgct caatactgcc gctaagatcc acagcagaaa tgcgctttct
    31441 tttaccgcca gggtgaggcc cagcccaata cccaatttgt ccattggcgg agtgaaatac
    31501 gggaaatagt cataaagcag ccctccgtca gcaaagatca gaagggcgct ggtggcaaaa
    31561 gccacatggg gaatggcgag cagccacggc agacgggcgc acatacgctg ccatttcggc
    31621 cctggccaca gcgccacaat caccagcagg gcaatcaaca acgccccgac cgccgcaatg
    31681 gttgtcgaca ccagcgttgc cagtaatgcc tgcggtaact gtggatcggc aaacaacgcc
    31741 tgccagtgcg tcaacgacaa agcaggcgat atcagcgtca gagctgccgg gatcagcggt
    31801 gcataaatca ccgctatcat cgcccacagc aaaaagatta atgcgtaccg taacggtgta
    31861 gccattcttg ttccagcgca tttacccaac ctgcgtgcgg ttcagccagt accggcggca
    31921 gatcctgcgg cattctcgat tgcaatgttt cgcgctgccc gtcaggcagt ttttgcggat
    31981 cgagaacaga aggatcgccc cagacagcgg gatctgcttt acgcagttgc gcatcgggtg
    32041 agagcaggaa attggcaact accttcgccg cagcactggc attcgcgttg gcaggaatgg
    32101 tgacgaaatg gacgttgcca atcatcccct cgcgaaaacc aaaactgtaa ctgcttgcag
    32161 gcaaatcgcc gctggcgatt ttttgctgcg catgtgcagg gttaaaggtc agcgacaggc
    32221 gcaatgtgtt agctttcagc agagcatcca tccgcgcggg cgaaggcggg aaatcctttc
    32281 cttcgcgcca taaatacgga tgcagtgcat caagatattg ccacaagggc gcagtgacgc
    32341 gggcgaaagt cgcatcgtcc ggcgcttctt ttaatgctgt gggatctggc gttagcataa
    32401 tcagcaactg ttcaagaaac gccgtgccgg taaagtccgg tgggcgcgga taggtaaccg
    32461 tgccgggatt ggctttagca aactccagta aggtttgcgg cgtttgtggt ggctgtggcg
    32521 taacatcgcg gcgggcgata aatgtcagtt gtgcgccgcc ccagggcgat tcagcccctt
    32581 ctgtaggcac tgaaaaatct tcccgcaccg gaagctgtgt gtcgacatag cgccagttgg
    32641 gcagagtctc cgcccagccc gtttgtaata aattggcctc ttttaaggtg cggaagtttt
    32701 cgccgttcac ccagagcaga tccaccgaac cgcccgtttt gcgtccggca gcagcttcgg
    32761 tctgaatgcg cttcaccgcg tctgcggcat ccgcgagacg aacaatcttc aggtttatag
    32821 cgtaatgggt tttcatctcg ccgctaaccc agtcgagata gcggttaatt gcggtatcgc
    32881 cgccccaggc gttaaaccag acggtttgtc ctttggcctc attttttatt tcctgccagt
    32941 cactggcatg tgttgccaaa gaaaacagcg ataacaatcc cagcaaccac ccacaatggc
    33001 gcatcttctc tccttaagaa cgtttttcag gaatacggca cagcccctga gcgggaatgt
    33061 ccatggcaga attaaaacgg gctgacagat tttgcaggcc tatcaatgcc gtcagttcgg
    33121 tgagcgcctg agcgtcaaaa tgcgtagcca gtcgggcgcg cagggcatca tcgaccgttg
    33181 gcggcgttac gcttgcggct tcggcatact ccagcgccag ccgttcttca tcgctaaaga
    33241 gcgggctttg ccgccagtca gccactgcca gcagtttatc ggtgctgccg gtacgctcgg
    33301 cgactttcat actggtgata tccacgcaaa actcacacag gcacatttgc gcaatgcgcg
    33361 cgctgacaag cgatcgtact accggatcga gcggtgagcg tttgcgctcc agccagccga
    33421 caaacatcga caccagataa aagataaacg ggatccgccc ccaccagcga atggggctta
    33481 gcacctcgcc gtaatggcga cgatgcagcc acgcctgcgg acgtaaaatg aaaggaattt
    33541 tgctaagcgg cggtaaaccc atcaggcatt cctcttctgg cgctcaagcc atattttctt
    33601 cgccataaaa atcaccacgg tcagggcaaa cagaataaac agtccggtga caaaccagaa
    33661 agtaccgcct gttaacatgc tgcccgccca ggaataaaca atagtcgccg gtaattgacc
    33721 aagcccggtg gcgataaaaa acgagcgaaa acgtattgaa gtcaaaccgg cagcatagct
    33781 gattggatcg aaagggacaa aaggcaataa ccgacatacc aggatggtgt gtttgccgta
    33841 acgagtgaaa aagccgtcca tactgtcaag tacggttttg ccggttaatt tttccaccac
    33901 ttcgcggccc atcactctgg cgataaaaaa gcacagtgcc gcgccggcca tcgaactggt
    33961 ccacgacagt aagccgcccc agaacgcacc aaacagcgac gcattggcaa aggtgatcaa
    34021 aaacgcaggt agcggtgcag caatggcctg taaaatcatc aacaagaacg agacaactgc
    34081 cgctagtgcg ccgtaagact gaataaagcg ttctataccc tgttggtcca cggcggcaaa
    34141 cgccgcaacg ctgcgattga taaactcatg gacgccggga agcagcgccc atgccagcag
    34201 cacagcgaac aacaggatga tgagggttat acggtagtac cagattttac gcgattgcat
    34261 cttcatcatc cgttatcctg ctttattcgg tgattttaaa ggtaaccgtt gccagcgtgt
    34321 tatccgccgg gagaactgag gcattgcctt tgaatttcac ttcgccgcgt ttttccaccg
    34381 caccataagt gtatgttgca ttgctgacga tgccgaccgg gcagctatcc agacacacca
    34441 ggcaacccgt tttcttctct tctgctgccg ttaaattacc gccaaagcgc atgtccagtt
    34501 tcttgccatt actgtcaaca atcacttcat cgaaggaata cgcttttgcc gccccttgcc
    34561 agttgaccga aatatccagt ttgctgcctg tgacatgagt cgtttcttta ttatccatcg
    34621 tcatgttttc gcccggcgta ccacctgcct ctttcagggc ttcataaaat gctttcgggg
    34681 tcgcataacc cataaacagc gatttatgtc cgttggagcc atctttaaac acaataccgt
    34741 gacgagtgtc gtcggtgagg aaacgaccat taatttgcac cagcatggtt accgtatggt
    34801 tattggcatc gacacgcagc ggttggctgt tcgaaagtcc gtcgtattcc acttttgcag
    34861 ccgcattctc tttttgatcg cagccgctaa gcccagcaac agccagtaag cagagtgcag
    34921 ccagtccttt tttccatgac actgaataat gttgcaacat cgttctatcc ttcatttttt
    34981 gggtgagaaa gtgggctgcg gcgagaagca gacagatcca catgtttgtg tcgggcgtag
    35041 agttttgcga gctggacgag aataaacagc gccaggcccg ccagacagag ttgtaaaata
    35101 aagcgcagcg taatgccttc attggcgaga tcgcttgcca tcacggtata aataacaata
    35161 ccgggcaggg tcgtgagtgc tgaaataagg gtataaggcc agaaggcgat tgtggttaat
    35221 ccgtaagcgt aattttgaat attgtaagga aacaacggga ttaagcgggt cagaataaga
    35281 aaatcaatac cgttacgcgc aatgcctttt tcaatggcct gaaaggtatg gctatgacca
    35341 acgtatttca gcagtaaatc acgtcccagc cagcgcgcca tcaggaatga gcacgaggag
    35401 gccagcgtgg cggcaattaa tgatagtagt gtccctaaga gcgggccaaa aactattcct
    35461 cccgcgatca ccagtatgct tcctggtaac agaaagaggg tggcaatgat gaataacaga
    35521 atatagaggc tatagccgaa caatccgctc tggcgaatga gtgtctgtaa gtggggtaaa
    35581 tcggtgagca gatcgaataa accgaaagcg tggatagcgt aaatgaccag cgcaaaaata
    35641 agacaggcta aaagaaattt acgctcagcg ttcatcgtgg agtttcctat atcaaacttg
    35701 ataatagaga agtggtcaga caactaaaaa cgcagtgacg gtataacaaa ggacggcagg
    35761 caacaaatca ttactgacag tttgttgatt tttctcaagg ttaattctcc tgacccagtt
    35821 tgagccagga gatcagctaa attagcggcg gaaggtcgcc cagacggggg cgtgatcgga
    35881 cggtttttcc atgctgcgaa tttcatagtc gatgccggtt tctacgcaac attctgccag
    35941 cggttggctg gcgagcagca ggtcgatgcg cagaccgcga ttatcgtcaa aaccttttga
    36001 gcggtaatca aaccatgaga aacgatctgc tgtttgcgga ttcgcatggc ggaaggtatc
    36061 gaccaacccc cagctcatca gcctctccat ccattcgcgt tcctccggaa ggaaagaaca
    36121 ttttccggta cgcagccagc gcttacggtt ctcttcgcca atgccaatat ccaggtcgcc
    36181 aggactaata ttcatatcgc ccataatcag caccggattt tcacgcttga gttcggtttc
    36241 caggtagttt tgcagattct gataaaactg cgccttcgca gggaatttta tcggatggtc
    36301 gcggctttca ccctgcggga agtaaccgtt gataacggtg acattgccca gcggcgaggg
    36361 gatttccgcc ataataatcc gccgttgcgc ctcttcgtcg tcaccaggaa agccgcgacg
    36421 cacggcaatc ggcgtctctt tggtcagcag tgccacgcca taatggcctt tctgcccgtg
    36481 ataaaacacg ttgtagccga gcttcgccac ctcttcgagc ggaaacatat cgtcatgaac
    36541 ttttgtctcc tgcaggccaa tcacatccgg ttggtgcttt tcgacgatgg cttcaagctg
    36601 gtgaggtctg gcgcgcaggc cgttgatatt aaaagagaca aatttcatag tcgctgccat
    36661 ttattcagat aaagagtgcg tggatggtag cagaatttcg ctttgcttac cgcctgttgt
    36721 taagtgattt gatcgatggt tgttaatgat gtcaacgatg gcgcaaaaaa tgcccgcttt
    36781 tggtgcgcgt tgcgtcagaa tggcgcagta atttccagta aatttcgtca atgatcacaa
    36841 ttccagaatt atattttact tctgcataac attgcgtttt ttattctttt tattcaccca
    36901 catcgcaaac gtattcactt tatatgcact ttaaatgcat atgatgtggt tataactgct
    36961 tgatttccag ttagcctccg ccgtttatgc acttttatca ctggctggca cgaaccctgc
    37021 aatctacatt tacagcgcaa acattactta ttattaacat ataaataacg aattatttac
    37081 tgtagaggtc gctatgtctc agccaattac gcgtgaaaac tttgatgaat ggatgatacc
    37141 tgtttacgct ccggcaccgt ttataccggt acgtggcgaa ggttcgcgct tgtgggatca
    37201 gcaggggaaa gagtatatcg acttcgcggg tggcattgcg gtgaacgcgc tgggccatgc
    37261 gcatccggaa ctgcgtgagg cgctgaacga acaggcgagt aagttctggc ataccggcaa
    37321 cggttacacc aacgagccgg tactgcgact ggcgaaaaaa ttgatcgacg ccacgtttgc
    37381 cgatcgcgtc ttcttttgta actccggtgc ggaagccaac gaagcggcgc taaaactggc
    37441 gcgtaaattc gctcacgacc gctacggcag ccataagagc ggcatcgtgg cgttcaaaaa
    37501 tgcgtttcat ggtcgcacgc tgtttactgt cagtgcgggt gggcagccag cctattcaca
    37561 ggattttgcg ccactgccgc cggatattcg tcatgctgca tataacgata ttaactctgc
    37621 cagtgcgctg attgacgacg ccacctgtgc ggtgattgtc gaacccatcc agggggaagg
    37681 cggtgtggtg ccagccagca acgcattttt acaaggtctg cgtgaattgt gtgaccgcca
    37741 caatgcgttg ctgatctttg atgaagtaca aaccggtgtc gggcgcaccg gggaactgta
    37801 tgcctatatg cactacggcg tgacgcctga tctgttaact accgccaaag cgctgggcgg
    37861 cggtttcccg gttggtgcgt tgttggcaac cgaagagtgc gccagcgtga tgaccgttgg
    37921 cactcacggc accacctatg gcggcaatcc gctggcctcg gcggtggcag gcaaagtgct
    37981 ggatctcatc aacacgccag agatgcttaa cggggttaaa cagcgtcacg actggtttgt
    38041 tgagcgtcta aattccatta atcaccacta cagtttgttc agtgaagttc gcggcttagg
    38101 tcttttgatt ggctgtgtac tgaatgccga ttacgccggg caagcgaaac agatctctca
    38161 ggaagcggtg aaagcaggcg tgatggtgct gattgcgggt ggcaacgtgg tgcgctttgc
    38221 gcctgcgctc aatgtcagcg aagaagaggt gacgaccgga ctggatcgct ttgcagctgc
    38281 ttgcgaacac tttgttagcc gaggttcatc atgatggtca ttcgtcccgt tgagcgctca
    38341 gatgtctcgg cgctgatgca gcttgccagc aaaacgggcg gcggcctgac gtcgcttccc
    38401 gccaatgaag ccacgctttc ggtgcgtatt gaaagggcaa tcaaaacctg gcaaggcgaa
    38461 ctgcccaaaa gtgagcaggg ttatgtgttt gtactggaag atagtgagac aggcactgtg
    38521 gcggggattt gcgccattga ggtggcggtt gggctgaacg atccctggta caactatcgt
    38581 gtcggcacgc tggttcacgc ttcgaaagag ctgaatgtct ataacgcatt gccgacgctg
    38641 tttctcagta acgatcacac cggcagcagc gagctgtgca cgctgtttct cgacccggag
    38701 tggcgcaaag agggcaacgg ctatttgctg tcgaaatcgc gctttatgtt tatggcggct
    38761 tttcgcgaca agtttaatga caaagtggtc gccgaaatgc gcggggtgat tgacgaacac
    38821 ggctattcac cgttctggca aagcctcggt aaacgcttct tttcgatgga ttttagccgc
    38881 gctgattttc tctgcggcac cgggcaaaag gcatttattg cagaactgat gccgaaacat
    38941 ccgatctata cccacttttt atcccaggaa gcccaggacg tcatcggtca ggtacatccg
    39001 caaaccgcgc ctgcccgtgc ggtgctggag aaagaaggtt ttcgctaccg taactatatc
    39061 gacatctttg acggcgggcc gacacttgaa tgtgatatcg accgcgtgcg cgtcatccgt
    39121 aaaagtcggc tggtggaagt ggccgaaggg cagcctgcgc agggcgattt tccggcctgc
    39181 ctggtcgcca atgaaaatta tcaccatttc cgcgtggtgc tggtgcgtac cgatccagcg
    39241 accgagcgtt tgattttaac cgccgcacaa ctggatgccc tcaaatgcca cgcaggggat
    39301 cgcgttcgtc tggtgcgcct gtgcgcagag gagaaaacag catgacttta tggatcaacg
    39361 gtgactgggt aacgggccag ggcgcattgc gtgtgaagcg taatccggta tcgggcgagg
    39421 tgttatggca gggcaatgat gccgatgcag ctcaggtcgg gcaggcttgt cgggctgccc
    39481 gtgcggcgtt tccgcgctgg gcgcggctct catttggcga tcgtcaggta agagttgaac
    39541 gctttgctgg attgctggaa agcaataaag ccgaattaac cgcgattatt gccagagaaa
    39601 ccggcaagcc gcgctgggaa gcggcaaccg aagtgacggc gatgatcaat aaaatcgcga
    39661 tatcaattaa ggcgtatcac gttcgtaccg gtgagcggcg tagcgaaatg ccggacggag
    39721 cagcgagcct gcgacatcgc ccgcacggcg tgctggcggt gtttgggccg tataatttcc
    39781 ctggtcattt gccgaacgga catatcgttc cggcattgct ggcaggtaac accattatct
    39841 ttaaacccag cgaactgaca ccgtggagtg gcgacgcggt aatgcgttta tggcagcagg
    39901 ctggcttgcc gccgggcgtg ctgaacctgg tgcagggcgg gcgtgaaacg ggtcaggcgc
    39961 tgagtgcgct ggaggatctc gacggcttgc tgtttaccgg cagcgccaat accggctacc
    40021 agctgcatcg ccagctttcc ggtcagccgg agaaaattct cgcccttgag atgggcggta
    40081 ataacccgct aattatcgat gaggtggcgg atatcgatgc cgctgtccat ctgaccattc
    40141 agtcggcgtt tgtcactgcc gggcaacgct gcacctgcgc ccgccgttta tttctgaaaa
    40201 gtggaacgca gggcgatgca tttcttgctc gcctggttgc cgtcagccag cggttaacgc
    40261 cgggcacttg ggatgacgaa ccgcagccat ttattggtgg gctgatttct gagcaggccg
    40321 cacagcaggt ggttactgcc tggcaggaac tggaggcgat gggcggacgg accctgcttg
    40381 cgccgcgctt attacaagca gggacatcgt tgctgacgcc ggggatcatt gaaatgacag
    40441 gcgttactgg cttgccggat gaagaagtgt ttgggccgtt attgcgcgtc tggcgttatg
    40501 acaatttcga tgaagcgatt cgaatggcga ataacactcg cttcggcctc tcttgcggtc
    40561 tggtttcccc cgagcgggaa aaattcgatc aactgttgct ggaggcacgg gcggggattg
    40621 ttaactggaa caaaccgctt accggtgctg ccagtaccgc gccattcggc ggcattggcg
    40681 cttccggcaa tcatcgcccc agcgcctggt atgccgcaga ttattgtgcc tggccgatgg
    40741 caagtctgga gtcggactcg ttaacgttgc ccgcaacgct taaccccggg ctggattttt
    40801 ccgatgaggt ggtgcgatga acgcctggga agtcaatttc gacgggctgg tagggctgac
    40861 gcaccattac gcgggcctgt cgtttggtaa tgaagcctct acccgtcacc gttttcaggt
    40921 gtctaacccg cgactggcgg cgaagcaggg cttactgaaa atgaaagccc ttgccgacgc
    40981 gggattcccc caggcggtga tcccgccgca cgagcgtccg tttattccgg tgctgcgtca
    41041 gttgggattc aggggtagcg atgagcaggt actggaaaaa gttgcacgcc aggcaccgca
    41101 ctggctttcc agcgtcagtt ccgcttcgcc aatgtgggta gccaatgcgg caacgatcgc
    41161 gccatccgcc gatacgctgg atggcaaagt gcatctcact gttgctaacc tgaacaataa
    41221 atttcaccgt tcgctggaag cccccgccac cgaatcgctg ttaaaagcga tttttaacga
    41281 tgaagagaaa ttcgctgtcc attcggcgtt gccgcaggtg gctttgctcg gtgacgaagg
    41341 agccgcgaat cataatcgtc tcggcggtca ttacggtgaa ccgggtatgc aactttttgt
    41401 ttacgggcga gaggagggca atgatacccg accgtcccgc tatccggcgc ggcagacccg
    41461 tgaagccagc gaggcggtgg caaggttgaa tcaggtgaat ccccaacagg tgattttcgc
    41521 ccagcaaaac ccggacgtta tcgaccaggg cgtttttcat aatgacgtga ttgccgtgag
    41581 taaccgccag gtgctgtttt gccatcaaca ggcgttcgct cgccagtcac agttactggc
    41641 aaatctgcgt gcacgggtca gtggttttat ggcaatagag gtgcccacgg cgcaggtttc
    41701 cgtgtcagat gcggtgtcta cttatctgtt taacagccaa ctgctgagcc gcgatgatgg
    41761 ttccatgatg ttggtgctgc ctcaggaatg tcgggaacat gctggagtat ggcgttatct
    41821 caatgaattg cttgctgccg ataacccaat aagctcacta aaagttttta atttacgtga
    41881 aagcatggcg aatggcggtg gcccggcgtg cctgcggttg cgcgtggtgt tgacagaaga
    41941 agaacgccgg gcggtgaatc cggcggtgat gatgaacgat acgctgttta atgcgctcaa
    42001 tgactgggtg gatcgttact accgcgatcg tcttactgcg gcggatcttg ccgatccaca
    42061 attgctgcgc gaaggaaggg aagcgctgga tgtattgagc caattactga atctcggttc
    42121 ggtttacccg ttccagcgcg agggaggggg aaatggataa ttttcttgct ctgaccttaa
    42181 cgggtaaaaa atcggttatc accgagcgag aaatcaacgg cgttcgctgg cgctggctgg
    42241 gcgatggcgt gctggaactg acgccattaa cgccaccgca aggcgcgctg gtgatttcag
    42301 cggggatcca cggtaatgag acggcacctg tggaaatgct ggacgcgttg cttggcgcga
    42361 tatctcacgg tgagatcccg ttacgttggc ggttactggt gatcctcggg aatcctcctg
    42421 cgctgaagca agggaaacgt tattgccata gcgatatgaa tcgaatgttt ggcggtcgtt
    42481 ggcagctatt tgctgaaagc ggggaaacct gtcgggcgcg cgaactggaa cagtgcctgg
    42541 aagattttta tgaccagggc aaagaatctg tgcgctggca ccttgatcta cataccgcaa
    42601 ttcgtggctc cttgcatccg cagttcggtg tattaccgca acgcgacatt ccctgggacg
    42661 agaaatttct gacgtggctg ggtgcggcgg ggctggaggc gctggtgttc catcaggagc
    42721 ctggtggtac gtttacccat ttcagcgcca gacattttgg cgcgctggcc tgtacgctgg
    42781 aacttggcaa agcgttgccc tttgggcaaa acgatcttcg ccagtttgca gtaactgcca
    42841 gcgcaattgc tgcgctgcta tctggtgaga gtgtcggtat cgtgagaaca ccgccgctcc
    42901 gttatcgggt ggtttcgcaa attactcgcc actcgccgtc cttcgaaatg catatggcaa
    42961 atgacacgct gaattttatg ccgtttgaga aaggaacatt gctggcgcag gacggggagg
    43021 aacgttttac cgtaacccat gatgtagagt atgtgttatt ccctaatccg ttggtagcgt
    43081 tgggattacg tgcgggatta atgctcgaaa aaataagcta atacttacc
//
LOCUS       DADSZD010000019        41664 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.019, whole genome shotgun sequence.
ACCESSION   DADSZD010000019 DADSZD010000000
VERSION     DADSZD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.019"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            249..2387
                     /gene="nrdD"
                     /locus_tag="J1Z28_000933"
     CDS             249..2387
                     /gene="nrdD"
                     /locus_tag="J1Z28_000933"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBA7715789.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            2545..3009
                     /gene="nrdG"
                     /locus_tag="J1Z28_000934"
     CDS             2545..3009
                     /gene="nrdG"
                     /locus_tag="J1Z28_000934"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBA7715790.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIIRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     MWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(3054..3440)
                     /gene="cybC"
                     /locus_tag="J1Z28_000935"
     CDS             complement(3054..3440)
                     /gene="cybC"
                     /locus_tag="J1Z28_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBA7715791.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AVQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(3623..4975)
                     /gene="pmbA"
                     /locus_tag="J1Z28_000936"
     CDS             complement(3623..4975)
                     /gene="pmbA"
                     /locus_tag="J1Z28_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBA7715792.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            5069..5620
                     /locus_tag="J1Z28_000937"
     CDS             5069..5620
                     /locus_tag="J1Z28_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBA7715793.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(5778..7151)
                     /gene="mpl"
                     /locus_tag="J1Z28_000938"
     CDS             complement(5778..7151)
                     /gene="mpl"
                     /locus_tag="J1Z28_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBA7715794.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            7327..8325
                     /gene="fbp"
                     /locus_tag="J1Z28_000939"
     CDS             7327..8325
                     /gene="fbp"
                     /locus_tag="J1Z28_000939"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBA7715795.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(8358..9353)
                     /gene="yjfF"
                     /locus_tag="J1Z28_000940"
     CDS             complement(8358..9353)
                     /gene="yjfF"
                     /locus_tag="J1Z28_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBA7715796.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGILLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(9340..10362)
                     /locus_tag="J1Z28_000941"
     CDS             complement(9340..10362)
                     /locus_tag="J1Z28_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7715797.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            complement(10376..11878)
                     /locus_tag="J1Z28_000942"
     CDS             complement(10376..11878)
                     /locus_tag="J1Z28_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7715798.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(12018..12974)
                     /gene="ytfQ"
                     /locus_tag="J1Z28_000943"
     CDS             complement(12018..12974)
                     /gene="ytfQ"
                     /locus_tag="J1Z28_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBA7715799.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEILVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            13284..13814
                     /gene="ppa"
                     /locus_tag="J1Z28_000944"
     CDS             13284..13814
                     /gene="ppa"
                     /locus_tag="J1Z28_000944"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBA7715800.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(14228..15700)
                     /locus_tag="J1Z28_000945"
     CDS             complement(14228..15700)
                     /locus_tag="J1Z28_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA7715801.1"
                     /translation="MKKSLLAVMLTGLFALVSLPALGNVNLEQLKQKAESGEAKAQLE
                     LGYRYFQGNETTKDLTQAMDWFRRAAEQGYTPAEYVLGLRYMNGEGVPQDYAQAVIWY
                     KKAALKGLPQAQQNLGVMYHEGNGVKVDKAESVKWFRLAAEQGRDSGQQSMGDAYFEG
                     DGVTRDYVMAREWYSKAAEQGNVWSCNQLGYMYSRGLGVERNDAISAQWYRKSATSGD
                     ELGQLHLADMYYFGIGVTQDYTQSRVLFSQSAEQGNSIAQFRLGYILEQGLAGAKEPL
                     KALEWYRKSAEQGNSDGQYYLAHLYDKGAEGVAKNREQAISWYTKSAEQGDATAQANL
                     GAIYFRLGSEEEHKKAVEWFRKAAAKGEKAAQFNLGNALLQGKGVKKDEQQAAIWMRK
                     AAEQGLSAAQVQLGEIYYYGLGVERDYVQAWAWFDTASTNDMNLFGTENRNITEKKLT
                     AKQLQQAELLSQQYIEKYAPEAWARMQKLKAQSAVKTGNK"
     gene            complement(15850..16191)
                     /locus_tag="J1Z28_000946"
     CDS             complement(15850..16191)
                     /locus_tag="J1Z28_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBA7715802.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(16194..19973)
                     /gene="tamB"
                     /locus_tag="J1Z28_000947"
     CDS             complement(16194..19973)
                     /gene="tamB"
                     /locus_tag="J1Z28_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBA7715803.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(19970..21703)
                     /gene="tamA"
                     /locus_tag="J1Z28_000948"
     CDS             complement(19970..21703)
                     /gene="tamA"
                     /locus_tag="J1Z28_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBA7715804.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21910..22548
                     /gene="msrA"
                     /locus_tag="J1Z28_000949"
     CDS             21910..22548
                     /gene="msrA"
                     /locus_tag="J1Z28_000949"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBA7715805.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            22871..24214
                     /locus_tag="J1Z28_000950"
     CDS             22871..24214
                     /locus_tag="J1Z28_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA7715806.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(24275..24481)
                     /locus_tag="J1Z28_000951"
     CDS             complement(24275..24481)
                     /locus_tag="J1Z28_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBA7715807.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            24806..25363
                     /locus_tag="J1Z28_000952"
     CDS             24806..25363
                     /locus_tag="J1Z28_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003830421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBA7715808.1"
                     /translation="MTLSKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(25353..26093)
                     /gene="cysQ"
                     /locus_tag="J1Z28_000953"
     CDS             complement(25353..26093)
                     /gene="cysQ"
                     /locus_tag="J1Z28_000953"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBA7715809.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVHAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            26283..28226
                     /gene="cpdB"
                     /locus_tag="J1Z28_000954"
     CDS             26283..28226
                     /gene="cpdB"
                     /locus_tag="J1Z28_000954"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBA7715810.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(28355..28735)
                     /locus_tag="J1Z28_000955"
     CDS             complement(28355..28735)
                     /locus_tag="J1Z28_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBA7715811.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            28824..29684
                     /gene="qorB"
                     /locus_tag="J1Z28_000956"
     CDS             28824..29684
                     /gene="qorB"
                     /locus_tag="J1Z28_000956"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBA7715812.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKPIT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            29792..30757
                     /locus_tag="J1Z28_000957"
     CDS             29792..30757
                     /locus_tag="J1Z28_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7715813.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLSCVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            30865..31527
                     /gene="ytfE"
                     /locus_tag="J1Z28_000958"
     CDS             30865..31527
                     /gene="ytfE"
                     /locus_tag="J1Z28_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBA7715814.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(31572..32984)
                     /gene="cycA"
                     /locus_tag="J1Z28_000959"
                     /gene_synonym="dagA"
     CDS             complement(31572..32984)
                     /gene="cycA"
                     /locus_tag="J1Z28_000959"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBA7715815.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWICMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(33293..33913)
                     /locus_tag="J1Z28_000960"
     CDS             complement(33293..33913)
                     /locus_tag="J1Z28_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA7715816.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            34131..34769
                     /gene="ytfB"
                     /locus_tag="J1Z28_000961"
     CDS             34131..34769
                     /gene="ytfB"
                     /locus_tag="J1Z28_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBA7715817.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(34753..35400)
                     /locus_tag="J1Z28_000962"
     CDS             complement(34753..35400)
                     /locus_tag="J1Z28_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7715818.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKQNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            35613..36404
                     /locus_tag="J1Z28_000963"
     CDS             35613..36404
                     /locus_tag="J1Z28_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBA7715819.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            36414..37256
                     /locus_tag="J1Z28_000964"
     CDS             36414..37256
                     /locus_tag="J1Z28_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBA7715820.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            37266..38042
                     /locus_tag="J1Z28_000965"
     CDS             37266..38042
                     /locus_tag="J1Z28_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBA7715821.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTALRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQVANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            38052..39593
                     /locus_tag="J1Z28_000966"
     CDS             38052..39593
                     /locus_tag="J1Z28_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HBA7715822.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQELVKSNAIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRKVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELAAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SSQEVLYITERAVFQLTAEGVELIEIAPGVEIDRDILPYMAFRPIIRHPRLMESSLFT
                     PMEDA"
     gene            39590..41662
                     /locus_tag="J1Z28_000967"
     CDS             39590..41662
                     /locus_tag="J1Z28_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBA7715823.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARMVIDPQGKCLP
                     QTRRGAKEEWRFRSELAEDKNHKLTIQYSQGSFITEVKSLRMQPCINGIYFEKNWPDF
                     LKGDTYTQ"
BASE COUNT        10097 a        11121 c        10500 g         9946 t
ORIGIN      
        1 tgagctacat caaaaaaagt tcaaacatcc ttgatgcaaa gcactatata tagactttaa
       61 aatgcgtccc aacccaatat gttgtattaa tcgactataa ttgctactac agctccccac
      121 gaaaaaggtg cgacgttgtg gataagcgga tggcgattgc ggaaagcacc ggaaaacgaa
      181 acgaaaaaac cggaaaacgc ctttcccaat ttctgtggat aacctgttct taaaaatatg
      241 gagcgatcat gacaccgcat gtgatgaaac gagacggctg caaagtgccg tttaaatcag
      301 agcgcatcaa agaagcgatt ctgcgtgcag ctaaagcagc ggaagtcgat gatgccgatt
      361 attgcgccac tgttgctgcg gttgtcagcg agcagatgca gggccgcaac caggtggata
      421 tcaatgagat ccagaccgca gttgaaaacc agctgatgtc ggggccatac aaacaactgg
      481 ctcgtgctta catcgagtac cgtcacgatc gcgacattga acgtgaaaaa cgcggtcgcc
      541 tgaaccagga gatccgtggt ctggtcgagc agaccaactc ctcgttactc aacgaaaacg
      601 ccaacaaaga cagcaaggtg attccaaccc agcgcgacct gctggccggg atcgtggcta
      661 aacactatgc acgccagcac ctgctgccgc gtgacgtggt gcaggcacat gaacgtggtg
      721 atattcacta tcacgatctc gattactcac cgttcttccc gatgttcaac tgcatgttga
      781 tcgacctgaa aggcatgctg acccagggct ttaaaatggg gaacgccgag attgaaccgc
      841 cgaaatcgat ctctacggca accgcggtaa ctgcgcagat tattgctcag gtcgccagcc
      901 atatttatgg cggcaccacc attaaccgta tcgatgaagt gctggcaccg tttgtcaccg
      961 ccagctacaa caaacatcgc aagaccgcag aagagtggaa catcccggac gccgaaggct
     1021 acgctaactc tcgtaccatc aaagagtgct acgatgcctt ccagtcgctg gagtacgaag
     1081 taaacaccct gcacaccgcc aacggtcaaa caccgtttgt gacctttggt tttggtctgg
     1141 gcaccagctg ggagtcgcgc ctgattcagg aatctatcct gcgtaaccgt atcgccggcc
     1201 tcggtaaaaa ccgtaaaacg gcggtgttcc cgaaactggt atttgcgatt cgcgatggcc
     1261 tgaaccataa aaaaggcgat ccgaactacg acatcaaaca gctggcgctg gagtgcgcaa
     1321 gcaagcgcat gtatccggat atcctgaact acgatcaggt agtgaaagtc accggttcgt
     1381 ttaaaacccc gatgggctgc cgcagcttcc tcggcgtgtg ggaaaatgaa aacggcgagc
     1441 agatccacga tggtcgtaac aacctcggcg tgatcagcct gaacctgccg cgtattgctc
     1501 tggaagcaaa aggcgatgaa gccaccttct ggaagctgct ggatgaacgt ctggtgttgg
     1561 cacgtaaggc gctgatgacc cgtatcgctc gtctcgaagg cgtgaaagcg cgcgtagccc
     1621 cgatcctcta tatggaaggc gcttgtggcg tgcgtctgaa tgctgacgac gacgtttctg
     1681 aaatattcaa aaacggtcgt gcgtctattt cgctgggtta catcggcatc cacgaaacca
     1741 ttaacgcgct gttcggcggc gagcatgttt acgacaacga gcagctgcgc gcgaaaggta
     1801 ttgctattgt tgagcgtctg cgtcaggcgg tggatcagtg gaaagaagaa actggctacg
     1861 gtttcagcct ctacagtacg ccgagtgaga acctgtgcga ccgcttctgc cgtctcgata
     1921 ccgctgagtt tggcgtggtg ccgggcgtga ccgataaagg ttactacacc aacagtttcc
     1981 acctcgatgt ggagaagaag gtgaacccgt acgacaagat cgactttgaa gcgccttacc
     2041 cgccactggc gaacggtggc ttcatttgct atggcgagta tccaaacatt cagcacaacc
     2101 tgaaggcgct ggaagatgtc tgggattaca gctatcagca tgtaccgtat tacggcacca
     2161 acacaccgat cgatgaatgc tacgagtgtg gctttaccgg cgaatttgag tgcaccagca
     2221 aaggcttcac ttgtccgaaa tgtggtaacc atgacacctc ccgtgtgtcg gtgactcgcc
     2281 gcgtgtgcgg atatttaggt agcccggatg cacgtccgtt taacgctggt aagcaggaag
     2341 aagttaagcg ccgcgttaaa catttgggaa atgggcagat aggttaatct tcactctgtg
     2401 ttaagcaaat gccggatgcg gcgtaaacgc cttatccggc ctacatttgg caacggctgt
     2461 aggcctgata agacgcgaca gcgtcgcatc aggcgttgat taccggatgc agcctacacg
     2521 cggactgata agccaggtga aataatgaac tatcatcagt actatcctgt cgacatcgtc
     2581 aacggccccg gcactcgctg caccctgttt gtctccgggt gtgttcatga atgccccggt
     2641 tgctataaca aaagcacctg gcgggtaaat tccggtcagc catttaccaa agcaatggaa
     2701 gaccagatca ttaacgatct gaatgacact cgcatcatac gccagggtat ttccctctcc
     2761 ggcggcgatc cgctgcatcc gcaaaacgtg ccggatattc tgaaactggt acaacgcatc
     2821 cgcgccgagt gtccgggtaa agacatctgg atgtggacag gctataaact cgacgaactc
     2881 aacgctgcgc aaatgcaggt tgttgatttg attaacgtgc tggtcgacgg caaatttgtg
     2941 caggatttaa aagacccttc cctgatctgg cgcggcagca gcaatcaggt ggtgcatcat
     3001 ttgcgatgat aagttccctg ttacctgagt gcaggcaaca gggaaataaa gaattaacga
     3061 tacttctggt gataggcgtt gcgggtcgtt ttcagttgct ctgcggcagc ctgcgcttct
     3121 tttactttac cttcatttgc cagcttcagc gcgtcgtcaa tctgaccgac cagaatgtcg
     3181 aaaccgtggc ggaagtcttt catttccggg ctgtccggtg atttgccttc aagcttcggc
     3241 ggcgtcgctt tttgcgcatc cagcgccgca gcgcgcattt tagttaacgc gtctttgact
     3301 tgcaccgcgt tatcggcttt ttcgaccact tttaaattgt cgttgagggt ttccatattg
     3361 tcttccagat cagcggcaaa cgacgccgaa ctgaatacca atgaggagac tgcaagaata
     3421 gctaacaggc ttttacgcat tgctcacttc cttttttatt tacacttcat ccttcacact
     3481 gcctcatcga cggctacgct cattcgcccg agtcacgtac tacgagtacg ctccccggct
     3541 ctcattcact ttccgtctcg atgcagtgcg aatgattttg tgtaattttt tagtttatag
     3601 cgcggcaggt cgcgccagtt ttttactgcc cggcgatttt catctccggc aacagcacag
     3661 aaccacactg tatattactg cgtgtttcaa tatcgttacc gacggtgaca atattgcgcc
     3721 acatatcttt taaattacca gcgatggtga tttcgctcac cggatactga atctcgccat
     3781 tctctaccca gaagcccgct gcaccacggg aataatcacc ggtaatggca ctcacgccct
     3841 ggcccatcag ctccgtcacc accagcccgg tgcccatctc tttgagcatt tgttcgaagc
     3901 ttagcccttg tccggcaatc cgccagttgt gaataccgcc cgcatgtccg gtgcttttca
     3961 gccccagttt ccgcgccgaa tagctggtca gcagccactg agtcaggatg ccatctttga
     4021 cgatatcgcg gcgctcggtg cgcacacctt cgctgtcgaa tggtgtagat gccagccctt
     4081 tcagcagatg cggatgctct tcaatggtca gccaatccgg cagaatttgt ttacccagcg
     4141 aatccagcaa gaaggtcgat ttacggtaga ccgcaccacc cgctatcgcc cccaccaaat
     4201 gaccaaacaa ccctgttgcc acttcattgg caaaaatcac cggcgctttc atggtagaaa
     4261 gtttacgcgg tgacaggcgc gataaggtac ggcgagcaca gtcggccccg acccactctg
     4321 gcgtttgcag atcgctcatc gcacgcccaa tggtgtaggc gtaatcacgc tccatatcgc
     4381 cattttcttc ggcgattaca caactggaga gcgaatgacg cgttgagcag taaccctgca
     4441 acatgccgtg gctgttgcca aaaactttga caccgtagtg gctgttaaag ctaccgcctt
     4501 cggtgttggt aatgcgttta tccgcctgta atgccgcctg ttcagcacgg gccgccagtt
     4561 caatggcttc atccggagaa acgtccgcag ggtggaacaa gtcgagatcc ggtgcgtcaa
     4621 aggccagcag ttctttgtct gccacgcctg catacggatc tggcgaagtg taacgggcaa
     4681 tatccagcgc cgcctgtacg gtgcgggcaa tggcctgcgg gcttaaatcg gtagacgatg
     4741 cgctaccttt acggttctgg tgataaacgg tgatccccag cgcgccatcg ctattgaatt
     4801 caacattctc cacttcacca taacgcgtgc ttacgctaat gccggtggtc ttgctgacgg
     4861 caacttctgc accgtccgat ttgcctgagg ccaactccag cgcagtcgaa actgcttctt
     4921 ccagaatctt gcgctgcgct tcaacttgag agattacttt cattgcaagt gccataatgt
     4981 agagagtttc tctgaagtct aacagagaac cgtttttcag tgcgcatctt aactggtaac
     5041 attagcctct ttttttaagg agcctgagat gactaagcaa cccgaagact ggctcgacga
     5101 cgttcccggt gatgacatcg aagacgaaga cgatgaaatt atctgggtca gtaaaagtga
     5161 aattaaacgc gatgccgagg agctaaaacg cctgggcgca gaaattgttg atctggggaa
     5221 aaacgcgctg gataagatcc cgttagatgc ggatctgcgc gcggctattg agctcgccca
     5281 gcgtattaag atggaaggtc gccgccgcca gttgcagctc atcggtaaga tgctgcgcca
     5341 gcgcgatgtt gagccgatcc gtcaggcgct ggataagctg aaaaaccgtc acaaccagca
     5401 ggtggtgctg ttccataaac tggaaaacct gcgcgatcgt ctgatcgacc agggtgatga
     5461 cgccatcgct gaagtattga atctgtggcc cgatgccgat cgtcagcaac tgcgaactct
     5521 gatccgcaac gcgaagaaag agaaagaagg gaataagccg ccgaaatccg cgcgccagat
     5581 tttccagtat ctgcgtgagt tagcggaaaa cgaagggtaa ttttcgttgt tgatgtgagt
     5641 atggtttgcc ggatacggtg cacctgtagg cctgataaga cgcatcaagc gtcgcatcag
     5701 gcactatgct caaatgccgg atgcggcgtg aacgccttat ccggcctaca cacactctat
     5761 caggctgaag ctgtgaatta ctgcgcagct tccgctttct tcgccagccc atccagcagt
     5821 ttctgatgga tcccaccaaa accgccgttg ctcatcacca gaatatggtc gccaggctga
     5881 gcggttttca ccaccatatc tgccagcgta tccacatcgc cgctccagtg tgcaggctga
     5941 acgcaggctt ctgccacttc tgccacctgc cacggaatat gtgccggttg cagcaggaag
     6001 acttcatcgg cacgacctaa tgaaggtgcc agatcgtctt tgcagatccc cattttcatg
     6061 gtattcgagc gaggttccag cacggcaata atgcgcgccg taccaccaac tttgccacgc
     6121 agcgccgcaa gcgttgccag aatcgccgtc gggtgatggg caaaatcgtc atataccgtg
     6181 acgccattcg cttcaccacg caactccaga cggcgacgag cgttaataaa cgaacccagc
     6241 gcgttagcgg catctgccgg cgctacacca acatggcgag ccgctgcaat cgccatcagg
     6301 ccattgtgca tattatgttc gcctaccagc gaccatttca cttcgcccac tttttcgcca
     6361 tccagcaaga cttcccattc ggaagcatcg gtggtcagct ttttcgcctg ccagtgcccc
     6421 tgctcgccca ccagctcctg ctcgctccag cagcccatcg ccatggtttg tttcaggttg
     6481 atgtcgtttt ccggccagat aatacgcccc tgccccggaa cgatacgcac cagatggtgg
     6541 aactgtttct ggatcgcttt caggtcgtca aagatatcgg cgtgatcgaa ctcaaggttg
     6601 ttgaggatca gcgtacgcgg gcagtaatgg acaaatttag agcgtttgtc gaagaaggcg
     6661 cagtcgtact catccgcttc gataacaaag aagtcgcttt cgcccagacg cgccgaaacc
     6721 tcaaagttcc ccggcacacc gccgatcaca aatcccggtt tgtaaccaca ctgttccaga
     6781 atccaggtcg ccattcccgc cgtggtggtt ttgccgtgtg taccggcaac ggccagcacc
     6841 cagcggtcgc gcagcacaaa atcgtgcagc cactgcggac ctgacatata agggatgttt
     6901 ttttccagta ccgcttccac acacggattt ccacgggtca tggcgttacc aataatcacc
     6961 agatccggct gtggatcgag ctggctggca tcgtaaccct gaatcagttc aatgccttgc
     7021 ttctcaagta aggtgctcat cggcggatac acattggcgt ccgaacccgt tacttcatgg
     7081 cctaactggc gcgccagcat cgccagaccg cccataaacg tgccacaaat tcctaaaata
     7141 tgaatgcgca tacgtcacta tcctttttta atctggcgct cattttacgc atatgtcggg
     7201 caagtgagaa acgcatttca ggataatccg taatttgctg gcgcgattca cctgagcgca
     7261 acattgggat tatttgttaa gattgttgcg gtcgctttac tccataaaca ttgcagggaa
     7321 agttttatga aaacgttagg tgaatttatt gtcgaaaagc agcacgagtt ttctcatgct
     7381 accggtgagc tcactgcttt gctgtcggca ataaaactgg gcgccaagat tatccatcgc
     7441 gatatcaaca aagcaggact ggttgatatc ctgggtgcca gcggtgctga gaacgtgcag
     7501 ggcgaggttc agcagaaact cgacctgttc gctaatgaga aactgaaagc cgcactgaaa
     7561 gcacgcgata tcgttgcggg cattgcctct gaagaagaag atgagattgt cgtctttgaa
     7621 ggctgtgaac acgcaaaata cgtggtgctg atggacccac tagatggctc gtccaacatc
     7681 gatgttaacg tctctgtcgg taccattttc tccatctacc gccgcgttac gcctgttggc
     7741 acgccggtaa cggaagaaga tttcctccag cctggtaaca aacaggttgc ggcaggttac
     7801 gtggtatacg gctcctctac tatgctggtt tacaccaccg gatgcggtgt tcacgccttt
     7861 acttatgatc cttcgctggg tgttttctgc ctgtgccagg aacggatgcg cttcccggag
     7921 aaaggcaaaa cctactccat caacgaagga aactacatta agtttccgaa cggggtgaag
     7981 aagtacatta aattctgcca ggaagaagat aaatccacca accgcccgta tacctcacgt
     8041 tatatcggtt cactggtcgc ggatttccac cgtaacctgc tgaaaggcgg tatttatctc
     8101 tacccaagca ccgccagcca cccggacggt aaactacgtt tactgtatga gtgcaacccg
     8161 atggcattcc tggcggaaca agcgggcggt aaagcgagcg atggcaaaga gcgtattctg
     8221 gatattatcc cggaaaccct gcaccagcgc cgttcgttct ttgtcggcaa cgaccatatg
     8281 gttgaagatg tcgaacgctt tatccgtgag ttcccggacg cgtaagctcc tgcgcctctc
     8341 cacacggaga ggcgttttta tcgctgcgca atgttgactc ttgtcactgg cgaactctga
     8401 cgattctccc acagcaccgt taatccacgc tgtaatgcta taaaaataaa caacaaaata
     8461 ccgatggcga ttttcgtcca ccaggaactc agcgtgccat caaagtttat gtaagtctga
     8521 atcagtccct gaatcgccac gccaaaaagc gtccctaata ccgttccaac gccaccactc
     8581 aaaagcgtac cgccaattac cactgaggcg atagcatcca gttccacacc tacgcccgcc
     8641 agtgcatatc cggcctgggt ataaatcgag aagacaatcc ccgccagcgt tgccagtccg
     8701 gtggagagca tataaatgcg aatagtggtg ctgcgagtgg aaatccccat caggtttgcc
     8761 gacgttgcgt tgccgccaat ggcgtatacc tgattaccaa aacgggtacg atgcgcgagt
     8821 aatatgccga taaccaccac cgccagcatc agtagtccca tcgcacttaa gcgaccaccg
     8881 ccagggattt tccacgcaag gcttgagagc gtgtcataaa caggatggtt tatcgggatc
     8941 gactcttccg aaacgagata gctgacgcca cgcaaaaaga acattcccgc aagggtaatg
     9001 ataaacgccg ggatcttcag ggcatcaatc agaagcccca taaatgcgcc gaaggcacag
     9061 cccatcacca acaccagcgg aaacgccagc agcggcgaga ggccgaaatc gccaatcact
     9121 tttgccagaa acacgccggt aaaggcgatc accgaaccga cggagagatc gatcccaccg
     9181 gagaggatca caaaggtcat gccaacggcg attatcccaa gaaaggcatt atcggtcagg
     9241 atattgcaga tcactcttgt ggaagcaaaa ccgggaaact gggtcagaca gtagatataa
     9301 cccaacacaa agacgccgat ggtgatcatc agtggcaaat tacgttttat cacggttacg
     9361 taccccttta atcagactga taaagcgttg cgactgaaca atcagcacgc aaagcaccac
     9421 caccgctttc accacctggt tcatctccgg cggaaagcca gaaagcaaaa ttccggtgtt
     9481 cattccctga ataatcagcg cccccaccac cgaaagcagc aggttaaagc gcccgcccat
     9541 cagcgatccg ccgccaatca ccactgcaag aatggcgtcc aactccagcc ataacccggc
     9601 gttattggca tcggcaccgc gaatatccgc cgccacgata atgcccgcaa tcgccgcaca
     9661 tagcccgctc aaaacgtagg tgagcatgac gatgatccgc gtgtttaccc cggcattttt
     9721 tgccgcccga atgttgatac caacggcttc gataaacatc cccagtgccg ttttgcgggt
     9781 caacagccag aacaggagaa gcgtcagcac cgcaataatg actggcgttg gcaggaacaa
     9841 cagcgatcca ctgccaaacc aggagagatc cggtgagtta aatgtgacga tctgtccgga
     9901 ggtgatcagt tgcgccacgc cgcgcccggc gaccatcagg atcagggtgg cgacaaacgg
     9961 ctgaattttg aggatcgcta ccagtatgcc gttccacaat cccgccagga tgccagtgcc
    10021 gagggcgctt aacaaaacaa ttggcaggct gaatcccgcg accgtcatcg cagccgttgt
    10081 ggctccggcg atagccatca ccgcccctac ggagagatca atcccaccgg tggcgataac
    10141 cagcgtcata ccaatcgcca gtagcgcaac gggggccgca cggttaagaa tgtcgatagg
    10201 gctaccgaac aaacgcccat cctgaagcac cacctgccag aaatgcgggg ccaccaggct
    10261 atcaaccagc agcaccagca acagtgccgc cagctgcggc attcctgttg gccagcgaaa
    10321 acgccttttc ggcggcgtag tgtccgggag agattgaggc atcacactgt tctccttacg
    10381 ccgcaatggc gttcatgatc gccggaacgg aaagctctgc cagtgggatc tccgctacct
    10441 gtttgcgatc gcgcatgata atcacccggt cggcatagcc caccagctct tccagctcgg
    10501 aggagatcac cagcagcgcc agcccatcgg cgcacagcgt ttcaatcagg cggatgatct
    10561 cggcgtgggc accaacatca attccgcgcg ttggctcgtc gagaatcaga aattgcggtc
    10621 gggtcagtag ccaacgtgaa agcaacactt tttgctgatt gccgccggag agaaattcaa
    10681 tcggttgttc ggtggaaggt gtgcgaatgc caagctggcg aataaagcgt tcggcaatct
    10741 cttgctgctc tttgcgggaa attggtcgca gccagccgcg ctgggcctgg agagcgagga
    10801 tgatattttc ccgcaccgag gcggcggcga taatgccatc ggttttcctg tcttccgggc
    10861 agaagccgat gcccagtacc gaagcctgat gtggcgagcg tagcgtttgc agtttgcctt
    10921 tgatcaacgc cgtgccgcta tcagcaggtt tgataccgaa gatcacttcg gcggtttcgg
    10981 tacgtcctga tcccagcaag cctgccagac cgacgatctc gccggggcgt acttcgagat
    11041 caaacggcgc gatcgttcct tttttgccgt aatttttgaa cgcggcaaca ggtttgtcgc
    11101 tcagcaatgt tctcccggca cgctgaagcg cgtgggtgtc cagttcacgc cccagcatca
    11161 tttttaccag ttcgatctgc ggtagctcgc gggtttcccg acagcccaca aaactgccgt
    11221 tacgtaagac ggtgatccga tcgctaacct gataaacctg atcgagaaaa tgagtgacga
    11281 agatcaggct gacgccgcga tcgcgaagct gacgcatcag gccaaacagt aactctactt
    11341 cctgagtgtc gagactggcg gtgggttcat cgaggatcag cactttggcg gagagatcga
    11401 tagcccggca aatggcgacg atttgctgca tcgcgactga aaagcggttg agcggttcgc
    11461 gcacgtcgag ggagaaaccg taagatgcca tcagttcggt ggcgcgcttt tccatctctt
    11521 tacggcgtag aaggccgaaa cgtttgggtt cgcgacctat gaatagatta tcagcgaccg
    11581 acatattggg tagcaggttg acttcctgat agacggtgcc gatgccgagt tgttgtgcgt
    11641 gggcggtgtt tttcggtgag atagcctggc cttccagcca gatagtgccg cgatcggcgt
    11701 ggtatacacc ggttaatgct ttgattagcg tcgattttcc cgccccgttt tcaccgagca
    11761 gcgccatgat ttcgccacgg cgcaggctga aatcaacgtt gtctaacgct ttgacaccgg
    11821 ggaaaaattt acttaatcct tcggtgcgga ggatttcctg gtgttggtcg gtcgtcatgg
    11881 tttcccccgg tgtaaagcgt gctcccagta ggcatgataa gacgcggtaa gcgtcgcatc
    11941 aggcttcagc gcaccaccgc cggatgcggc gcgagcgcct tatccggcct acgaatcggc
    12001 atcaggcata gcaaccctca ataccccata tttttcttct tctctaactc ttcttttgcg
    12061 gtatcaggca gataaagggt ggatttggtc agggttagct tttcaggcat ggtgccgtct
    12121 tttttatatt tctccagcgc atcaaaagcg gggcctgcca tgttcggcgt cagttcaacg
    12181 ctggcgttcg cttcgccatc aatcatcgct ttgtagatgt ccggcacgcc gtcgatagag
    12241 cctgtcagga tatctttgcc cggcttcagg cccgcttctt taattgcctg aattgcacca
    12301 atcaccatgt cgtcgttatg ggcgtaaacc atgcagatgt ttttgccgtt gttttccgct
    12361 ttgataaagc tctccatgac ctctttgcct ttactacggg tgaagtcacc tgactgcgag
    12421 cggataattt tgatatttgg cgcattcttt atggcttcgg caaagccttt cttacggtca
    12481 atggcgacgc tggccccaac ggtgccctgc agctccacca cgttgcatgg tttgccattc
    12541 acttctttta ccagccagtc accgatcaac ttgccttcga ggatgttgtc ggcggtgaca
    12601 gtggtcatat agagagattt gtctttcaca tcgatggaac ggtcaagcaa gaagaccagg
    12661 atttcggcat ctttcgcctc ttttaatacc ggctcccaac cggtcgctac cactggagcg
    12721 ataaagatcg catccacccc ttgcgcgacg aaggaacgta ccgctttaat ctggttttcc
    12781 tgcttttgct gaccatcggc aattttcaac gtaattccgc gcttttcggc ttcacttttc
    12841 gccacattgg tttctgcggc gcgccagccg gattccgatc cgacctgcga aaatcctacg
    12901 gttaatggag cggccaacgc catagacgac atggctgccg agactgcaga gactacaagt
    12961 aagcgtttcc acatagggtt gtcctcgtcg gggtaattat tattggtgaa aagatgttcg
    13021 caaaaaaact atagacaatt cgttatgtaa cggattgcgt tacatcacac ttcaaaaaag
    13081 taaataaaac gttaatcaca agtttgtaat cgctttcatc tcactatgaa aaatgcggct
    13141 acggttatgg attttcctgc tctgtatacc gtcttaaaac tggcgaaaaa ggaaaatgaa
    13201 gacgaaaaca agcgaagaca ttcggcgcga gttggctata atactcggca cttgtttgcc
    13261 acatattttt aaaggaaaca gacatgagct tactcaacgt ccctgcgggt aaagatctgc
    13321 cggaagacat ctacgttgtt attgagatcc cggctaacgc agatccgatc aaatacgaaa
    13381 tcgacaaaga gagcggcgca ctgttcgttg accgcttcat gtccaccgcg atgttctatc
    13441 cgtgcaacta cggttacatc aaccacaccc tgtctctgga cggtgacccg gttgacgtac
    13501 tggtcccgac tccgtacccg ctgcagccgg gttctgtgat ccgctgccgt ccggttggcg
    13561 tactgaaaat gaccgacgaa gccggtgaag atgcgaaact gattgctgtt ccgcacacca
    13621 agctgagcaa agaatacgat cacattaaag acgttaacga tctgccagag ctgctgaaag
    13681 cgcaaatcgc tcacttcttc gagcactaca aagacctcga aaaaggcaag tgggttaaag
    13741 ttgaaggttg ggaaaacgca gaagccgcta aagctgaaat cgttgcctcc ttcgagcgcg
    13801 caaagaataa ataagttctt ctagcgtaat aaccctgaac gccgggcttc ggttagtagg
    13861 ggttttttta tgcccgcaat ttataaactc tctactccac tattattatt ttcagcagtt
    13921 gaaaggcttg aacggtaaga acaagctaag ctcgaccacc attttgctgt tcatagccac
    13981 ttgctggaag ttagccggcc tcactcatac tcaccgttca aagcgtgtcc ccgttttacc
    14041 cctgtacatt tgttaacgcc agcagagttt tttatgcgat tgcagatatg gaaagcggcc
    14101 cggagagaaa agcaaaagat gggaaagtta caaaaaaaaa tcgcgagata catcgcataa
    14161 aacaacgcca cccgcaggtg gcattggttg agctgtatta gcgtagacct gatctgacag
    14221 ttacccgtta tttattaccc gtctttaccg ctgattgcgc tttaagcttt tgcattctcg
    14281 cccaggcttc cggggcatat ttttctatat attgttgcga taataattca gcctgttgca
    14341 gttgtttggc tgtcagtttt ttctctgtaa tgttgcggtt ttctgtacca aaaagattca
    14401 tatcattggt cgatgcggta tcgaaccacg cccaggcctg cacataatca cgttctacgc
    14461 ccaagccata ataatagatt tcacctaatt gtacctgcgc tgcacttaat ccttgctctg
    14521 cggcttttcg catccagatt gcggcctgtt gctcatcttt tttaacacct tttccctgga
    14581 gtaaagcatt acccaaatta aattgcgccg ctttctcacc ctttgcggcg gccttgcgaa
    14641 accattccac cgccttttta tgttcttctt ctgaaccaag tctgaagtaa atagcgccga
    14701 gattagcctg tgcggtagca tccccctgtt ctgccgattt cgtgtaccag gagatggctt
    14761 gttctcgatt ttttgctaca ccttctgcgc ctttatcata cagatgtgcc aaataatact
    14821 gaccatcgga atttccctgt tctgccgatt tacggtacca ctccagtgct tttaacggct
    14881 ctttcgctcc cgctaaacct tgctccaata tatatcccag acgaaactgc gcaatagaat
    14941 ttccctgctc tgccgactgg gaaaataata cccgtgactg agtgtaatcc tgagtaacac
    15001 cgataccgaa atagtacata tcggctaaat gaagctgccc caactcgtcg cctgatgtcg
    15061 ctgattttcg ataccattgt gccgatatgg catcgtttct ttcaacgcct aaacctctgg
    15121 aatacatata accaagctgg ttacaggacc agacgttacc ttgttccgct gctttgctat
    15181 accactcacg tgccataacg taatcccgcg tcacaccatc gccttcaaaa tatgcgtctc
    15241 ccatactttg ctggccgctg tcacgacctt gctctgctgc caggcgaaac catttcacag
    15301 attcggcttt atcaaccttc acgccattac cttcatggta cattacaccc agattctgct
    15361 gcgcttgcgg aagccccttc agtgccgctt ttttgtacca gataactgcc tgagcataat
    15421 cttgcggcac accttcaccg ttcatatagc gtaaccccag tacatattct gccggggtgt
    15481 atccctgctc tgcggcacgg cgaaaccagt ccatcgcctg ggtgagatcc tttgtcgttt
    15541 cattaccctg aaaatagcga tatcccagct ccaactgtgc tttagcttct ccactttcag
    15601 ctttttgctt taattgttcg agattgacat ttcccagagc gggaagagaa acgagagcaa
    15661 acagtcctgt cagcattaca gccagaagag attttttcat tgtggtcgcg tccttgttga
    15721 gcgtgccagg tgtttataac actgcgcaac aaatcagcat ttaagaaaag ataagaatgg
    15781 cgtcaaattc ggaatatatt caggctggtg gataaaacaa cgccacccga aggtggcgtg
    15841 cttttattat tacttatccc tgtctaacca gtcgccgctt tcaattagct ttaatccatc
    15901 gacgggtcgt tgataaacgt acatccatgc actcccgtac ggcgtctgaa tcaactggcg
    15961 cgcgtattca ccgcccctgg tgcgcaaggc atcaagttcg gccagcgtgg cgttgtcaat
    16021 acgataaact tcaccatgta ctgttccgtt ccccggaact gcgcctggat agtggcccag
    16081 gctatacaac tggtagttat caatactgaa atcgcccagt aactgggcat tggtcatcca
    16141 gtgactgttg ccttgtttgt ggcgtaaact gccgtagaca aatattcgca ttgctaaaac
    16201 tcgaactgat agagcaaatc cagtgcctgg tctacaccag acacggcttc cagatatagc
    16261 ttaggcatca ggcgataacg taacgtgagt gttgctatag agtcaaatat acccacgccg
    16321 tatttcactt gcaggcctgg caatacatag ccgctgacca ccacctggga ggagtcgcca
    16381 actccctcgg tgtcgagcgc taaattgctt acgccaaacg tctcgccgat tttacccaca
    16441 atctggccac tttgcgcaac ccccaggcca atcagcatcg aggtcattgc cgcactgtcg
    16501 ctctgatcgc tctccagccc ttgtccacgt agcaaataag acaatgcagc ttgttgcgac
    16561 atcgccgggt cagagaagat ctccgctttc ggttcgtccg ccagaccagt gacgcgaact
    16621 ccggcgatga cgtcgtcttc tgtagcatcc gggttacgaa tagcttcgat attaagatac
    16681 ggttgatctg gcggaccaga gaacagtagc tcacccttac gcacaatcag atcctgacca
    16741 taggcatgga agcgcccttc agggatgttg atctgcccgt tcaggcccag cccttgtttg
    16801 tcctgaacga cgttgagatc gcccgtcagc cgcgctttca ggccaaaggc gtcaatgcgc
    16861 acattgttgc caacgtggac aatcaggtta ctgttaatcg gaatcgacgc cgttttcggc
    16921 tcttccggtt gcaggttatc gttaagcatc accacatcgc tggaaacgcc tactgcgctt
    16981 tccggcagat cgtgcaccac gatgcgcgcc cacggaacat ccacgcgacc atcgagggta
    17041 aacaggtttg gtgtagcctc gaatacaaca tctggcgata catccattcg taccatcggc
    17101 ggcacggtga tacgcacttt actgcccttc gctgttaccc gcgcccgcca gttttcaatc
    17161 tggctccagt cggcatcacc gttcaggtag atttcaccct gctgggtccg cacggtacct
    17221 gcaagcgtcg agcgcatacc gttaaagttg accgcaagct ggcttggttg catatcaaac
    17281 ggcataaagt tgccgtcgat atccaccccc gtaacctgaa gctgaccaaa caactgcggg
    17341 ctttgcacat caccacccag acgcaagttg gcactcacca tccccgctgc tttttcccca
    17401 cgggtaaaga tggggtttat catcgcaagg ttgaagttac ggatattgac gttgccacca
    17461 agattacggc ggccttgcgg atcggtcacc tgcacctgac catcaaactg gccgttattg
    17521 gtcaggcgga tggtccagcc caattcggca cggttgttac gcaactccgc cgtcagattc
    17581 agcgtctgaa acgccacggg cagcgccgca tcgttgacgg tttgcgtcac ctgcacatta
    17641 cgccccgaaa gagtgatact gccctgcggc agcccctctt tcgtggtgtc ccaggcaacg
    17701 tccgctttac ccgtgaagat accgctggcc tgagtggttt ctggcataaa tggtttcagc
    17761 atggccaggt cgaagcggtt gagattcacc accgcacgcc cttcggcacc cgcatcgata
    17821 gtttgcggca cgcacagttc cgcattcggg ttaagccagc agtgtggccc gatgctgatt
    17881 ttttgctcct tattgcggta atccagcgca atatcgcggg tcagcgacca cgggccgacc
    17941 ggcgtctgga agcgggtatt gctaagagtt cccttccagc gttcttcttt gcgatcaaaa
    18001 cttcctgcca gattaagctg cccggagaca ggctcgccct gaatccgcaa ctgtagctcg
    18061 tgctgctttt cactgccttt ggcattcagg gtgacgagat tgatatttac atccggttgc
    18121 gaaatttgct caacgcgtac gtcgagtttc ccggcgatct gatcggtgga tttaatgtcg
    18181 ccttccacgc gaacctgcgc cacggaaagt tcctgccagc gcaggccgcg cgcggtgata
    18241 tctgccagta gttgtggcgc ttccaccgtg ccgcgtactt tcaccagccc tttcgctgta
    18301 ccgccaagcc ccggcagcgc gttatccagc cccggcgcgt tgatggtggc atcaagattg
    18361 agatctttta cccccagctc gcctttcact tcggcactgt ttggccccag ttccagatgc
    18421 agccctggga tcatccactg catgtaactg ttgcctttca gcgtgccgtc aacgttcact
    18481 ttgttctgtt tgacgttacc ggtcagcttc agttctggca cctccatctg ccaggtgccg
    18541 ccgtacaggc taccgcgggt tttaatcaag ccattgagtt tcgacggcca ctccgggatc
    18601 tctttggcgg tgttaatgcc gttaagcgtt agctcaccgc gccaactaat ggcctgctgc
    18661 cagtcgagca acgccttaag ttccgttttc ccttccagcg ccgcgacggt aagtttgtcg
    18721 agattgacct gctgttcatt acctttggca tcaagggtaa tggtggctgg cgggatctcc
    18781 tggcccttaa cagccgtgcg catagagagc gtgtaatcgg tcattttgcc ggtaagtttc
    18841 agtttcagat catccgcctg atactgcttc tcaccagtga acggccagta aagctgtttg
    18901 ctgttcactt ccacgttgag cggcaatccg gcttccgcca gtcgcgtctg ggcgcgtaaa
    18961 tccatatcca ccggaccgga aaggttaacg cctatctcca gctgttcgcg cagcgcaccg
    19021 cccactttca gcttcacctt ttcacctttc aacggctcca cgttcagtgt gctgttgagg
    19081 gtgatatcca ccggccagtt gtctgacagc tgcgccgtgc cgctggcgtt gacgatccct
    19141 tgactggagt cgatatccag ggcgtccagt ttagtattgc cgtcaatgct gctcactttc
    19201 agcagcatgg tgctcacggt gatgtccgtg tcgcccgtca cgcgcagctg ctcgccctta
    19261 aactcttcaa tgttcaggtt aagcggcaaa tgcacgtcgg tcatttccgg caataccggg
    19321 cgagaaaaga gatctttcag cgtttcgccg agcggctttt cctccggctg tggattttca
    19381 attttcggtt cgaccacttc ttcctgcgcc gcttccgcca ctttcggcaa agcaatcaac
    19441 aggcctttca gcgatgtcgg tttcagggtc agggttttct cctgccagtt gaggccggag
    19501 gtgaagtcca tcaccgatac cgtggtgtca tcaatcttga tgttgacgtt gtccagcgcc
    19561 acccgtgtca gggtaatggg atacggcgtg gagagatcca gcggaccgct atcttcctct
    19621 tcttcaacct gttcagaagg aggcattttt ttactgtcga tgttgacctg aatgtctttc
    19681 agcgccagat cattaataca aacgctactg ttccacaggc actcaagccc gaccgccaga
    19741 tgcagattgc ccgcttttac cgccacgcct ggctgctcat aacgaacgtc agacaaggtg
    19801 agatcacgcc agccgccggt gaccttgcca atatccagtc ctggcaccca gcgatccgcc
    19861 gctttaaata ccagatgcag gccgctggtg gtgcccacca gaaacgccac cgatcccagc
    19921 aacagtaaga taacgataac cacgccgagg ctgatttttt tccataaact cataattctg
    19981 gccccagacc gatgtaaaac tgtaacccgt gttcgtcttt atccgcgacc ggcacggcaa
    20041 aatcgagttt gattggcccg accggcgatt cccagcgcac gccgacaccg gtaccggttt
    20101 taaagtcgct gcggcgaata tcgcttaccg cttcgccgct atcgacaaac accgcgcccc
    20161 accattttcc ggtcacgttg tactggtatt ccagcgatcc ggttatcagc ttcgaggccc
    20221 ctttcaggtc accgttggcg tatttcgggg cgatagattt gtatttgtag ccgcgaatac
    20281 tgcgatcgcc cccggcgaag aaacgcagat ccggcggtac tttgtcgaaa tcacccgttt
    20341 caatccagcc cagcgtgccg cgtgtcacaa aacgatggcg atcgtacagt gtgcggatcc
    20401 agacgttctg cgcctggaaa acggagaaat caacatccga gccccaggcc gtgttggagt
    20461 agtcgataga gtagcgttgc gagtcgcccc aggttggcat caggccacca cgagaacgcg
    20521 tgcggctaat catcacccca ggataaaaca gcatcgtggt gttggtaatt tcaccctggg
    20581 taaagtggtc gaaactccag cgcaggttaa tggcacgctg ccagccgctg gagagatccc
    20641 agtagcgaga agccaccagc gtagtggagt cagactcggt atcgttcagg tcagtgcgct
    20701 taaaaccgcc ctgcaccaaa taatattgtt ccagcggatt cttcagcagc ggcattttat
    20761 agctgaagtc gaggatctgt tccggcgcgg aaatactggt actggtggtc aaactgtgac
    20821 cgtaagagtt catccacggc tttttccacg tcgctttcac gcgcggtccc acgtccgtag
    20881 agtaaccgac gccggtttcg atggtgtttt ctgttcgcgg cgaaaccacg cccgtcaatg
    20941 gtaatacttt cgtttcgcgc gctttatcaa attgtggagc caccaccacc gagttaaacc
    21001 agccggtagc agaaagtcga cggttcagtt ctgccagatc tttcgattcg tactcatcgc
    21061 cctctttaaa cggcaccaga ttttgcaggt attcatcgcg gatttgtgat ccttcaaagg
    21121 tcacatgccc aaagcggtaa cgttcgccac tgttataatc aatatcccag aaggctttat
    21181 gcaggccgag cgcaatgccc agctgtgctt tggtaaattc gctatcgaaa taacctttac
    21241 gcaacgcaat gctggttaag gactttttga aattttcata atcaccctgg ttcagtaccg
    21301 tgccaatagc cgggcgagta tcgagcaatt tcaaatagtc tttatcggtc cgcgcgccgc
    21361 cgcgcaatac cacatcggtg ccgccaatta acaccggcac gcctggcgtg actttggcga
    21421 tcaatacctg ccgccctttc tttggcggtg gacggagatc aaattcaatg gtcggctggt
    21481 aatagcccag cgctttcaga ccttcgcgga tggcatcatc gacgcgtgcg cgaaagcgac
    21541 ggtctggcgt cacctcatca ctttcaatcg tagaaagctg cgcgcgaacg ttcttttcca
    21601 gctgtcccga taacccctcg acctgtagac ggacgttcgc ggcgacggca gatccgctta
    21661 agcagagtaa gcttacacag cataactgtc ggatatagcg cacattttct cctgaatatc
    21721 ctttttttcc tgcccctgga acgccgttaa acggcttaac aaaaatccag taatatggat
    21781 taaaaagcag actaaaccca aaatatttct tatgttttac tttagaccta ttcacggtgg
    21841 ttattgtgtg caaatacgcc tcttgttaca accttaaccc caatgaccga ttttcgggag
    21901 agcgacacca tgagtttatt tgataaaaag catctggttt cccccgccga tgccctgcct
    21961 ggacgtaaca ccccgatgcc cgtagccaca ctgcatgcgg tcaacggtca ctcaatgacc
    22021 aatgtacctg acggaatgga gattgccatt tttgcgatgg gttgtttctg gggtgtggag
    22081 cgtctgttct ggcagttacc cggcgtttac agcaccgccg caggctatac cggcggctat
    22141 acgccaaatc cgacttatcg ggaagtgtgc tccggcgata ctggccatgc cgaagcggtg
    22201 cgcattgttt acgatccatc cgtcatcagc tatgagcagt tgctacaagt attttgggag
    22261 aatcacgatc ccgcccaggg aatgcgtcag ggcaatgacc acggcacgca gtatcgttca
    22321 gcgatttatc cgctgacccc agaacaggat gccgccgctc gcgccagtct ggaacgtttt
    22381 caggcggcga tgcttgccgc cgatgacgat cgtcgcatca ccacggaaat cgctaacgcc
    22441 acaccgtttt attatgccga agatgaccac cagcaatatc tgcataaaaa cccgtatggt
    22501 tactgtggaa ttggcggaat tggcgtctgt ctgccgccgg aagcatagcg ttacgggtac
    22561 aaatgtagat tgttgataaa gtgcattttg tttatgccgg atacggcgta aacgccttgc
    22621 caggcctaca aaatcgtcca aattcaatat attgcgggga ctgcgtaggc ctgttcggca
    22681 tagcgcatca ggcagttttt catttttcag ctgtctgaat tttaaccctc tggcggcttt
    22741 acagtacctt acgctatact agccactgaa aatgccggtt cactttcttc gaatcggctt
    22801 tcaatgtgta tttcacacaa attaatcaac ttccccttcc gaggatctgg cctgaaaggt
    22861 cggataagat atgttaaaca gtattttagt catactctgt ttgatcgctg taagtgcgtt
    22921 cttctcgatg tccgagatct cgcttgccgc ctcacgcaaa atcaaactta aactgctggc
    22981 tgatgaaggc aatataaatg cccaacgcgt tttgaatatg caggaaaatc ccggcatgtt
    23041 ctttaccgtg gtccagatcg gtctgaacgc agtggcgatt ctcggcggta tcgtcggtga
    23101 tgcggcattt tctccagctt ttcacagcct gttctcccgc tatatgtcgg cagaactctc
    23161 tgagcaactg agctttattc tctctttctc gttagtgact ggcatgttta tcctgtttgc
    23221 ggatttaacc ccgaaacgca tcggtatgat tgcgccagaa gctgtggctt tgcgtatcat
    23281 caacccgatg cgcttctgcc tgtacgtttg caccccgctg gtgtggttct tcaacggact
    23341 ggcgaacata atcttccgta ttttcaaact gccaatggta cgtaaagatg acatcacttc
    23401 tgatgatatc tacgcggtag tggaagccgg tgcgctggcg ggcgtgttac gtaaacagga
    23461 acacgagctg attgaaaacg tctttgagct ggaatcccgt accgttccgt cttcaatgac
    23521 accgcgtgaa aacgtgattt ggtttgatct ccacgaagat gagcaaagtc tgaagaataa
    23581 ggtggcggaa catccgcact ctaagtttct cgtctgtaat gaagatattg accacatcat
    23641 cggctatgtc gattctaaag acctgctgaa ccgcgtgctg gctaaccaaa gcctggcact
    23701 gaacagcggc gtacaaattc gcaacacgct gattgtgccg gatacgttaa ccctttcaga
    23761 ggcgttggaa agttttaaaa ccgcaggtga agacttcgcg gtgatcatga acgagtacgc
    23821 gctggtagtg gggatcatca ccctcaacga cgtgatgacc acgctgatgg gcgatctggt
    23881 cggccagggg ctggaagagc aaattgtcgc ccgtgatgag aactcatggc tgatagacgg
    23941 cggcacgcct attgacgacg tcatgcgcgt gctggatatt gacgagttcc cgcagtcggg
    24001 caactacgaa accatcggcg gctttatgat gtttatgctg cgtaagatcc cgaaacgcac
    24061 cgattcggtg aaattcgccg gctacaaatt tgaagtggtg gatatcgata actaccgcat
    24121 cgaccagctg ttggtgaccc gaatcgacag caaagccacc gccctttcgc caaaactgcc
    24181 tgacgctaaa gataaagaag aaagcgtcgc gtaaccccag aaacatcaac ggctcctgaa
    24241 tcaggagccg tcttattact gcatagcact ttggttaagc catctctgtt tgcagacgca
    24301 taacctgacg gttaacttcg gacatcactg acaaatgcag cttatccttc acttttggga
    24361 taagaatctt acccttatca aattcaaaag cgccaacgtc cttgatgtat aaccgtccac
    24421 ggaacaggat ctttacgtac ttcgccacct gaagtgggtt gtaccgttgg aaaattttca
    24481 ttgttatctc ctgctgagta ttacgccttt gcggtgccac aatcggccca actattatga
    24541 ggcgcaaatt ttaatgccta gtgactatag actatccggg caatgtttcc accgcgtata
    24601 acttttttta cctaaaggtt acaattattc agaattatct ttttaccgaa gcgcggtctt
    24661 cagtataagc attcattttt catatgattt gtgcgcttga ccgcaaactg gcatcacact
    24721 tgcgggaaat tcgataaata gcacatatga ttaaaactca gacccaagtg gtcggatcac
    24781 ctgcatatca taagaaggaa acaccatgac cctaagcaag attctggcgc tcacctgcct
    24841 gctgttgccg atgatggctt ccgcacatca gttcgaaacc ggtcagcgag tgccgccgat
    24901 tggcatcacc gatcgtggcg agttggtgct tgataaagat cagtttagct acaaaacctg
    24961 gaacagcgcg cagttagtgg gaaaagtgcg agtactgcaa catattgctg gtcgcacctc
    25021 tgcaaaagag aaaaacgcga cgctgattga agcgattaaa tcagcgaagt taccgcacga
    25081 tcgttaccag accaccacca ttgttaacac cgacgacgcg attccgggtt cagggatgtt
    25141 tgtgcgcagc agtctggaga gtaataaaaa gctttatccc tggtcgcagt ttattgttga
    25201 tagcaatggc gtcgcacgcg gtgcctggca gctggatgaa gaaagttccg ctgtagtggt
    25261 gctggataaa gacggtcgcg tgcaatgggc caaagacggg gcgcttactc aggaagaggt
    25321 gcagcaagtg atggacctgc tgcataaatt aattaataaa tagacactct gaaccccgga
    25381 ttcaggaacg actcgcgcgg agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc
    25441 gctccggcag ctgcagccac agcatgacca gcggcggtat cccaaatatt cgttggcccg
    25501 aaacgcgggt acagctgcgc ctgtccttcc gccaccaggc agaatttcag cgaagagccg
    25561 atggacgtgg tctgatgttc gccaagctgt tgcagatact ctttcagctc cgcatctgca
    25621 tgagaacggc tgatcaccac cagcggcggg cgcgcatcgc ggacctgaat ctgcttgcgc
    25681 acaccgcact cttctttcca ggctttgcct tctgccgcgc tgtacattac gttcattacc
    25741 ggcgcatgca ccacacctaa aatcggtttg ccgtggtcga tgagcgcaat gttaacggtg
    25801 aattcgccat tacgtttaat aaactcttta gtaccatcca gcgggtctac cagccagtaa
    25861 cgctgccagt gctgacggac ttcccaaccg ggaggatctt cttcagaaag gaccggaata
    25921 tccggtgtca gcgtacgtaa accgtccatg ataacggtgt gagcggcaat atccgctgcc
    25981 gttaccggag aattgtccgc tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc
    26041 tgcataatgg catcgcctgc attccgtgca agctggcata cttgatctaa catttctcca
    26101 cctcgtctct gtgagcggtg ttaacttatt gttttactta taccctatcg ttaatgaatg
    26161 cgccaactgt gatagtgtca tcattttcaa agcgtaaaat tgtggcattc ttcactgttc
    26221 tataagtaag acgtttattc tcccttttct ttcgtattcc cgatgataaa aggatgtccc
    26281 ggatgattaa gtttagcgca acgctcctgg ccacgctgat tgccgccagt gtgaatgcag
    26341 cgacggtcga tctgcgtatc atggaaacca ctgatctgca tagcaacatg atggatttcg
    26401 attattacaa agacaccgcc acggaaaaat tcggactggt acgtacggca agcctgatta
    26461 acgatgcccg caatgaagtg aaaaacagcg tactggtcga taacggcgat ttgattcagg
    26521 ggagtccgct ggcagattac atgtcggcga aaggattaaa agcaggtgat attcacccgg
    26581 tctataaggc attaaatacg ctggactata ccgtcggcac gcttggcaac cacgagttta
    26641 actacggtct ggattatctg aaaaatgcgc tggcgggagc gaaattccct tatgtaaatg
    26701 ccaacgtcat tgacgccaga accaaacagc caatgtttac accgtattta attaaagaca
    26761 ccgaagtggt cgataaagac ggaaaaaaac agacgctgaa gattggttat attggcgtcg
    26821 tcccgccaca aatcatgggc tgggataaag ctaatttatc cgggaaagtg acggtgaatg
    26881 atattaccga aaccgtgcgc aaatacgtgc ctgaaatgcg cgagaaaggt gccgatgtcg
    26941 ttgtcgttct ggcgcattcc gggctgtctg ccgatccgta taaagtgatg gcggaaaact
    27001 cagtttatta cctcagtgaa attccgggcg ttaacgccat tatgtttggc catgctcacg
    27061 ccgttttccc aggtaaagat tttgctgata tcgaaggggc tgatatcgcg aaaggcacgc
    27121 tgaatggtgt tccggcggta atgccaggca tgtggggcga tcatcttggt gtggtcgact
    27181 tacaactcag taatgacagc ggtaaatggc aggtgacgca ggcgaaagcg gaagcacggc
    27241 cgatttacga catcgctaat aaaaaatccc tcgcggcgga agacagcaag ctggtagaaa
    27301 cactcaaagc cgatcacgat gccacacgcc agttcgtcag caagccaatc ggtaaatctg
    27361 ccgacaatat gtatagctat ctggcgcttg tgcaggacga tccgaccgtg caggtggtga
    27421 acaacgcgca aaaagcgtat gtcgagcatt acattcaggg cgatccggat ctggcaaaac
    27481 tgccagtgct ttcagctgcc gcaccgttta aagttggtgg tcgcaaaaat gacccggcaa
    27541 gctatgtgga ggtggaaaaa ggccagttga ccttccgtaa tgccgccgat ctttatctct
    27601 accccaatac gctgattgtg gtgaaagcca gcggtaaaga ggtgaaagag tggctggagt
    27661 gctccgccgg acagtttaac cagattgatc ctaacagcac gaaaccacag tcactcatca
    27721 actgggatgg tttccgcacc tataactttg atgttattga tggtgtgaat tatcagattg
    27781 atgttaccca gcccgcccgt tatgacggcg agtgccagat gattaatgcc aatgcggaaa
    27841 ggattaagaa cctgaccttt aacggcaagc cgattgatcc gaacgccatg ttcctcgttg
    27901 ccaccaataa ctatcgcgct tacggcggca aatttgccgg tacgggcgac agccatatcg
    27961 cttttgcttc accggatgag aaccgctcgg tgctggcagc gtggattgct gatgagtcga
    28021 aacgtgcggg ggaaattcac ccggcggcag ataacaactg gcgtttagca ccgatagctg
    28081 gcgataagaa actggatatc cgtttcgaaa cctctccgtc agataaagcc gcagcgttta
    28141 ttaaagagaa agggcagtat ccgatgaata aagtcgcgac cgatgatatc gggtttgcga
    28201 tttatcaggt ggatctgagt aagtaaaaca cttctttttc ggcctacaaa tcatcaaccg
    28261 catccggcat ttattggcgg atgcgatgat gacgcatctt atccgcccta caagccatga
    28321 accgtagtcc ggataagcgc agcgcatccg gcagttatgc cgcacgttca tcccgcaccg
    28381 ctaacacctc gggcaaattc aactcaatcc agtccgccag tgcagcaacc ttttcgctca
    28441 cctgctcgcc cagcggcgtg aggctatatt ccacatgcgg cggcaccacc ggatacgcga
    28501 tacggttaag aaaaccatcc tgttctaatg cctgtaacga ctgcgcaagc atcttttcac
    28561 tcaccccgcc cattttgcgc cgcaggtcgc taaagcgatg agtaccttcg cgtagcgcca
    28621 ccagaatcaa caccccccaa cggctggtga cgtgtttcaa cacctcgcgc gacgggcact
    28681 gttccgcaaa gagatttccc tctttcagtt gttgcgacag gctaacctga ctcatttcat
    28741 acttaccttt ttgtgcgtac ttactaaaag taagtttagg tgttagcgta tttaaacaca
    28801 agacaaaacg atggagactt cccatgatcg ctattactgg tgccactggc caacttggtc
    28861 actatgttat tgaatccttg atgaaaaccg ttcctgccag ccaaatagtg gctatcgttc
    28921 gtaatccggc aaaagcacag gcactgacag cacaaggcat taccgtgcgt caggctgatt
    28981 acggcgatga agccgcactg acatctgcgc ttcagggagt ggaaaaacta ctgctgatct
    29041 cttccagcga agtgggtcaa cgtgccccgc agcatcgtaa tgttattaat gccgcaaagg
    29101 cggccgacgt gaaatttatc gcttatacca gcctgctaca tgccgattcc tccccgctcg
    29161 gcctcgccga tgagcacatc gagacggaga aaatgctggc tgattctggc atcgtttaca
    29221 ctctgctgcg caacggctgg tacaccgaaa actacctcgc cagcgccccg gcagcactgg
    29281 aacacggcgt atttatcggt gccgcgggcg atggcaaaat tgcctcagca acgcgggctg
    29341 attatgcggt agctgcggcg cgcgtaatta gcgaagccgg tcatgaaggc aaggtttacg
    29401 aactggcggg cgatagtgcc tggacgctga cacagttagc ggcagaactc accaaacaga
    29461 gcggcaaacc gattacctat caaaacctga gcgaagccga tttcgctgcg gcactgaaaa
    29521 gcgtcggact gcctgatgga ctggcggata tgctggcgga ttctgacgtt ggcgcatcga
    29581 aaggcggtct gtttgatgac agcaaaacgc ttagcaaatt gattggccgc ccaacgacaa
    29641 cgttagccga aagcgtaagc catcttttta atgttaataa ctagttaatt aaagtggcat
    29701 cctcccgcat cctctctgat aatgacggga tgccgggagc aatcatgtct gcttcctgaa
    29761 ctttcttctg acagaccaat ggatgccagt aatgattagc ggcgtgctgt acgccctgtt
    29821 agcagggttg atgtgggggc ttatttttgt cgggccgttg atcgtaccgg aatacccggc
    29881 gatgctgcag tcgatggggc gttatctggc gttagggtta attgcgctgc ccattgcctg
    29941 gctgggacgc gtgcgtctgc gtcagttggc gcgtcgcgac tggcttaccg ccttgatgct
    30001 caccatgatg ggcaacctta tctattactt ctgccttgcc agtgccattc aacgtactgg
    30061 cgcgcctgtt tccacgatga ttatcggcac cctgccggtg gtgattcccg tttttgccaa
    30121 tctgctttat agccagcgcg acggcaaact cgcatgggga aaactcgccc cggcactggt
    30181 ttgtattggc atcggcctgt cgtgtgtgaa tattgctgag ttaaaccacg gactccccga
    30241 ttttgactgg gcacgttata cctcaggcat cgtgctagcg ttagtttccg tggtctgctg
    30301 ggcatggtat gccctgcgca acgcccgctg gctgcgggaa aatcccgaca aacatccgat
    30361 gatgtgggcg acggcgcagg cgctggtcac gctgccggtt tctctcatcg gctatctcgt
    30421 cgcctgttac tggctgaata tgcaaacgcc ggacttctcc ttaccctttg gcccccgtcc
    30481 gctggtgttt attagtctga tggttgcgat agccgtgctt tgctcatggg ttggcgcact
    30541 ctgctggaac gtcgccagcc agcgattacc gacagtgatt ctcgggccgc tgattgtttt
    30601 cgaaacgctg gcaggtttgc tgtacacctt tttactccgc cagcaaatgc cgccgctaat
    30661 gacgctgagc ggtatcgcgc tgttagtgat tggcgtggtc atcgcggtca gagcaaaacc
    30721 agaaaaacct ttaactgaat ctgtctcaga aagttgacac gctggcagtg agttaaataa
    30781 gcctctgcta cgtaagggtt atagcttttg ccttaaagat gcatttaaaa tgcatcttat
    30841 cttattaaga atgaggtatc agctatggct tatcgcgacc aacctttagg tgaactggcg
    30901 ctctctattc ctcgcgcttc ggctctgttt cgtaaatatg atatggatta ctgctgtggc
    30961 ggtaagcaga cgctggcgcg cgcggcggca cgtaaagaac tggatgttga cgtcattgaa
    31021 gccgaactgg caaagcttgc tgaacaaccg attgagaaag actggcgtag cgcgccgctg
    31081 gcggaaatca tcgaccacat tattgtgcgc tatcacgatc gccatcgcga gcagctgccg
    31141 gagctgatcc tgcaagcgac taaagtcgag cgcgttcacg ccgacaaacc aagtgtgcca
    31201 aaagggctga caaaatacct gactatgctg catgaagagc tttccagcca tatgatgaaa
    31261 gaagagcaaa ttctcttccc gatgattaaa caaggcatgg gcagccaggc aatggggcca
    31321 atcagcgtaa tggaaagcga gcacgatgaa gcgggcgaac tgctggaagt gatcaaacac
    31381 accaccaata acgtcacacc gccgccggaa gcctgcacca cctggaaagc gatgtataac
    31441 ggcattaatg aactgattga tgacctgatg gagcacatca gtctggaaaa caatgtacta
    31501 ttcccgcgcg cgctggcggg tgagtgatct aaaagctgga tggcattgcg ccatccagca
    31561 tgataatgcg attatttccg cagttcagca gcccgcttct taccaataaa cagccagccc
    31621 agccccagcg cgataaacca cagcggggta accagcagcg cctggcgggt gtcatcttcc
    31681 agtgtcaaca acaccagaac aaacacaaag aacgccatac atatccagca catcagcttg
    31741 ccgagtggca tcttgtagat cgacttctca tgcagatgag gacgctgttt gcgatacacc
    31801 aggtacgagc aaaggataat cgtccagaca aacataaaca gaatcgcgga aacggttgta
    31861 atcatcgtga acgcgccaat cacgctggga ttcacataca acatcaccac gccgccgagc
    31921 agacagatac aagagaaggt cagccctttc gcgggtactg cgcgcttaga gagtttagcg
    31981 aacgctttcg gtgccacacc ttcctgggcc agaccaaaca gcatacggct ggtagagaag
    32041 acaccgctgt tagcggaaga cgccgcagag gtcagcacca caaagttgat cacgctggca
    32101 gccgcaggca aacctaccaa cacaaacagt tcaacgaacg ggcttttctc cgggactacc
    32161 gaactccacg gcgtcacgga cataatcaca atcagcgaga agacgtagaa cataatgata
    32221 cggatcggaa tggagttaat cgcgcgtggc agtgatttct ccggatcttt ggtttccgca
    32281 gctgttgtac ctaccagctc aatccccacg aaagcaaaaa ccgctatctg gaatccggca
    32341 aagaagccgc ttaagccttt cgggaaccag ccgccgtcat tccacaaatg tgcaaatgat
    32401 gcttccacac cggtcggtga ctgaaagtgc atcgccacca tgaccaggcc gacaacaatc
    32461 agcgacacga tggcgacgat tttgatcatc gcaaaccaga actccatctc accgaacatt
    32521 tttaccgtgg cgaggtttag aaccagtaac agaatgatca cggataacga agcaacccag
    32581 tcagaaagcc cagggaacca gaattgcgca taggcggtaa tggcaaccac gtctgccata
    32641 ccggttacaa cccagcagaa ccagtaagtc cagccggtga aatatcctgc ccacggcccg
    32701 agtaaatcgg aagcgaagtc actaaaagat ttgtattcca gattcgaaag cagcaattcc
    32761 cccattgccc gcatcacgaa aaagagcata aaaccgatga tcatataaac gaaaatgatc
    32821 gacggcccgg caaggctaat cgttttgccg gaccccataa acagccctgt accaatggca
    32881 ccgccaatgg caataagctg aatatgtcgg tttgtgagat tgcgccgtag cgactgttca
    32941 gccggagcct gatcatcggc aacgactttt acctgatcta ccatgttttt ttcttcctgt
    33001 acctgtctgt gttgttcagg ctctacggcc tttagtcagt ctatgatacg acgaatctgt
    33061 atcgggactc atcgatatta ggtaacaata cgcgggatga atactaagat ttagatctaa
    33121 tgttaatttt atgtttaaag tgagtgtcat atcacggtga taacgcgaaa cagctcacaa
    33181 aaatacacgc ttacaggggc tttgcaacat aaaatagcgc cgtttggcaa actgaagggt
    33241 ttattgctga atgcctgctc ccctctggtt cgaggggaac agaatgcgct gcttagagga
    33301 tttccagcag ttcgacttca aacaccaggg tgctgaacgg agggatggac gcgcctgcgc
    33361 cacgctcgcc atatgccagt tcctgcggga tagtcagttc ccatttagaa cctaccggca
    33421 tcagagtcag tgcttcaatc cagccaggga tcacaccatt aaccgggaat tcagctggct
    33481 cgccgcgagc aacggagctg tcaaacacgg tgccgtcgat cagttttccg gtgtaatgaa
    33541 cacgaacgcg gtcggtacgt gccggaattg cgccttcacc ctggttgatc acgcggaatt
    33601 gcaggccaga ttcggtgcta tttacacctt cttttttggc gttttcttcc aggtatttca
    33661 caccttcagc agccatcgcc tggaaacgct gacgacgaac ggcatcggcg cgttcgtgga
    33721 tttcacgcag cgcgcggtgc accacatcaa ccggaacagc cggatgtttg ccttccagcg
    33781 catcggcaat gcctgcaacc agtgcttctg gcagcagccc ttccaggcca gattcactca
    33841 gttgttgccc tacctgcaaa ccaatgccgt agcttgcttg cgcttcgatg gtgtcaaaag
    33901 ttggggtggt catcgtattt cctttcttag ggtataaagt agcgggcagc atatcagcca
    33961 cacttctttg ggtaaaactt tgtcgcggga atgatgacaa atcccgggga aaaagaaaca
    34021 atagcaatat cccgtatgaa tataataaag gcgggtttac cacggacatc atagcgttag
    34081 cttattcata ggctatgatt gaggaacaag acgcggagca ggaggaaacc atgcccgggc
    34141 gctttgaact aaaaccaacc ctggagaaag tctggcacgc gccggacaat tttcgcttca
    34201 tggacccgct gccgccgatg catcgtcggg ggatcattat tgccgccatt gtattggtag
    34261 tcggatttct gcttccatct gatgatacgc ccaacgcgcc ggtggtgacg cgtgaagcgc
    34321 agttagatat tcagtcgcaa tcacaaccac cgacagaaga acagctgcgg gcgcaattag
    34381 tcacgccaca aaatgatcct gatcaggtgg ctccggtcgc tccagaacca atacaggaag
    34441 gccaaccaga agagcagccg caaaccaccc atacgcaacc attccagcca gatagcggaa
    34501 ttgataatca atggcgctcc tatcgtgtcg aacccggtaa aacgatggcg caactgttcc
    34561 gcgaccacgg actgcccgct accgatgtct atgccatggc gcaggtagaa ggcgcggata
    34621 agccactgag taatttgcaa aacgggcaga tggtgaaaat ccgccagaac gccagcggcg
    34681 tggtgacggg cttaaccatt gatacgggga ataatcaaca ggtcctgttt acccgccagc
    34741 cggatggcag ttttattcgc gcgcggtaga tgaagggtca accaaaagcc agctaaggat
    34801 cgtctctttg atgaccttac gtctggcttc cagtgcctgc ggtgaaacca tactgatgtg
    34861 ataaaccagg ccaagcgtat gctggttgat caaataatat cccccaagcg cagcaatatt
    34921 aatattcacc tgcaatgggt cgatatctgg cttaaaaata ttttgtttct tcccttcatc
    34981 caataaggat tccattaact gcaagtgcgc atggttaatt tctaaaagac gctgtgattt
    35041 tgcataatgc acacccttac tttgattttc actatgcaca atttttaaaa accagggatt
    35101 agaaatataa taatcccatg taaaatcgat tattttctca atggctttct gtggcggaag
    35161 atctgagaaa tcaagggcct tttctttttg tcgaatatcg ttccaggtat attccagtac
    35221 ttcgataaat aaattttctt tattaccaaa gtggtgataa accgtctgtt tactgcaccc
    35281 cgcggccttg acgatattat cgactctggc cccttcgtaa ccatattcgg caaattcatt
    35341 aaccgcgcat aacagcagct tttctttcag gctctgtacg ttgctggtat caagatacat
    35401 aacgcttccc cacgacctct tcgtggtgac tatcataacc tgatttgatt tcagcttcag
    35461 aaaaaagggc aaaaacttgt actttcatca ggttatcaaa tgtcctgtga tcgccatcgc
    35521 atatcaacca actagatggt tgatcttttt atcctccagc cgcaagctaa tcatcactaa
    35581 ggaaacaaaa ttcaaaaaaa gagagctatt gtatgaaatc cactcgtcct gttgccgtga
    35641 ttaccggagc cgctcgtggt attggaaagg gctgtgcgct ggagcttgcg cgtggcggat
    35701 ttaatctgct gattaatgat cgtccggatg cggatagcgt agaaaaatta catgccacac
    35761 aacaagaatg tctggcggaa ggcgtggaag tgatttgctt ccctgctgat gtgggcgatc
    35821 tctccctgca tgaagagatg ctcgacgcag cgcaaaacca gtggggacgt ctggactgcc
    35881 tgctcaacaa cgcaggcatt tcagtgaaaa aacgcggtga ccttctcgac ctcgaaccag
    35941 acagcttcga ccaaaatatt gccattaaca cccgcgcacc gttcttcctt gctcaggcat
    36001 tcagtaagcg cttactggca cagccgaaac cggaagcaga actgccgcac cgctcgatta
    36061 ttttcgtcag ttccatcaac gccatcatgc tggcgatgaa tcgcggcgaa tataccattg
    36121 ccaaaactgc cgtctctgcc gctgctcgtc tgtttgctgc tcgcctttgt aatgaacaaa
    36181 ttggcgtata tgaagtgcgt cctggtctca tcaaaaccga tatgactatt ccggcaacgg
    36241 cctattacga cgaactgatt gccaaaggtc tggtgccgtg gggacgttgg ggttatccgg
    36301 ctgatattgc ttccactgtc cgcgcgatgg cggaaggtaa actgatttac acctgtggtc
    36361 aggcggtcgc catcgacggc ggcttaagta tgccgcgctt ctgaggctga atcatgagag
    36421 atctgttaca acgcccggat ttattcagta tcaataccgc gacactgggc tataaaacgc
    36481 cgctacccgc cattattgat gcctgcgccg cacgcggtat tggcgcgatt gccccctggc
    36541 gcagggagtt gcagagtgaa gatctccagc aaatcgcccg ccagcttgct gccagtaaca
    36601 tgaacgtttc cggtttgtgc cgtagcacct actacaccgc accgacgctg gcggagcgca
    36661 aactggcgat tgacgataac cgccgggcgc tggacgacgc cgctgtactg aacgccgcct
    36721 gttatatgca ggtggttggc ggtctgccaa tgggaacaaa agatctgtac gaagcacggg
    36781 aacaggtgaa acaagggatt cgccagttgc tgccacattc gaaagaggtc ggcgtgccaa
    36841 ttgcactgga gccactgcac ccgatgactg ctgccgatcg ttcttgtctg tgcacgttac
    36901 gtcaggcgct ggactggtgt gatgaactcg atcccgatgg cgaatttggc ctcggcgtgg
    36961 cggtagatgt ctaccatgtc tggtgggatc cggatctcgc cagtcagatc ctgcgtgccg
    37021 gaaaacgcat cctcgcgttt catgtttccg actggttagt gccgaccacc gatctggtca
    37081 atgaccgtgg aatgccggga gatggtgtca tcaatattcc gtcaattcgt cggctggttg
    37141 aaaacgccgg atttaatggc gctattgaac tggaaatatt ctcaccgtac tggtggcaga
    37201 aagatattaa cagcacgctg gatattagcg tcgatcgcat cgcgcattat tgctaaggaa
    37261 taatcatgag tgaccaaccc gttctgttca gcagagcggc ggcttcttgc cgcctgacat
    37321 tgaaccgtga agataaatgt catgctatta atgaagaaat gatcgaatct ctcgaccatt
    37381 atttaaatga aattgaaaat gacacggcat tacgcttagt cgaattaacg gcaaccggcg
    37441 ataaattctt ttgtgcgggc ggcgatatta aatcgtggtc ggcctattcg ccattagata
    37501 tgggcagaaa atggattaaa cgcggtaatg acgtttttaa tcgcctgcgc aacttacccc
    37561 aattaaccgt tgcgaatctc aacggtcata ccatcggtgg cggtattgaa cttgccttgt
    37621 gctgcgatat tcgtattgcc cgccccggcg cgaaattctc taacccggaa gtgatgctcg
    37681 gcatggtgcc tggctggatg ggcattgaac gcgtgcttaa tcaggttggc ccggtggtcg
    37741 gtcgccagat gctgatgctg ggtaaacgcc tgacggcgca ggaagctcag gtggcgaatt
    37801 taattgatga agtggtagaa aaagaacagg tggaaagctg gatggcgaac cagttagcgc
    37861 agctggaaaa atgtggtccg gtggcactgg cacatattaa gcaactgatt ctggcgctgg
    37921 aaaataaaca cgccgattac tcccaccagt tactggctgg attaatgtcc gccactcagg
    37981 attgccaaca ggcgacgcac gcatttgccg aaaaagccgg cgtgagcttc cacaatcaat
    38041 aaggaaattg tatgcgtaag ttatcaaccg ccgagacact ggcggcgcaa attcaggacg
    38101 gcgcaaccat tgccattagc ggcaacggcg gcggcatggt ggaagctgac catattctgg
    38161 ccgccattga agcgcgcttt ctgcaaaccg gacacccgcg cgatctgaca ttaatccact
    38221 cgctgggtat tggcgatcgc gacagcaaag gcactaaccg ttttgctcac gccgaaatgc
    38281 tcaaacgcat tattgccgga cattttacct ggtcgcccaa gatgcaggaa ctggtgaaaa
    38341 gcaatgctat tgaagcctac tgtttccctg gtggcgtaat tcaggcactg ctgcgggaga
    38401 tcggtgccgg acgtccgggg ctttttaccc acgttgggct gggatcgttt gttgatccac
    38461 gcaatggtgg cggtaagtcg aatgaatgca cgaccgacga cctggtagaa ctgattgaaa
    38521 tcgacggtga aaccaaactc cgctatcgcc ctttcaaagt ggactacgcg attttgcgcg
    38581 gcacctatgc cgatccgcgt ggcaacgtta gcctcgaaga agaagcgatt gatatggata
    38641 gctattcgat ggcgctggca gcacacaaca gtggcggcaa agtgtttgta caggtgcgcg
    38701 atgtactgga agctggcgcc attgaacctc gcaaggtcaa gctaccgggg attctggtcg
    38761 atggtatcgt tgaacaccgc gaacaaccgc aaacctatct tggcggttac gacctgacca
    38821 tcagcggtca gcatcgccgt ctaagttcta acgacgctat tgaactggtt agtcatccgg
    38881 tgcgtcgcct gattgcccgt cgggcagcgc gggaactggc ggcaggtgct tcaaccaact
    38941 ttggctttgg tattcccggc ggtattcctg gggttgcact gcgcgaaggc gtgccttatc
    39001 aaagtttgtg gctgagtgta gaacagggtg tacataacgg catgatgctg gatgatgctc
    39061 tgttcggctg cgcccgtaac gccgatgcca ttattccatc actcgatcaa ttcgaattct
    39121 acagtggcgg cggtatcgat atcaccttcc tcggcatggg agagatggat cggtacggta
    39181 acgtcaacgt atcccatctc aatggcaatc tcattggccc cggcggcttc ctcgaaattg
    39241 cgcaaaacgc ccgtaaagtg gtgttctgcg gtacgttcga cgctaaaggc agcaagattg
    39301 atgtaacgcc tgatggcctg catatcgccc agtcaggtca aatccccaaa ctggttaccc
    39361 aggtggaaaa aattactttt agcgccgcat acgcacagca aagcagtcag gaagtgctgt
    39421 atatcaccga acgtgcggta ttccagttaa cggcagaagg cgttgaatta attgagatcg
    39481 cccctggcgt agagattgac cgcgacattc tgccgtatat ggccttccgt ccgattatca
    39541 ggcacccacg cctgatggaa agtagcctgt ttacgccaat ggaggatgca tgagtcagct
    39601 tgatgaggcc atccttgatg cactgacaca cgttactttc ccaaagggtt ttgcacaggc
    39661 agaacccgca tgggttgtca cggtagacgg tgttgattac ccactctgga aaacagatgc
    39721 cctggtagtc ggcagtggtg cagccgggct acgtgcagct gtggaactta aacgccgcca
    39781 gcaaaatgtg ttaatcgcca ccgccgggtt atatatgggg acatcggcat gttctggctc
    39841 tgataaacaa acactgttta ccgccgctac cgcgggcaac ggcgacaact tcgccaaact
    39901 ggcagaggca ctggcgagcg gtggggcgat ggatcacgac accgcttatg tcgaagcggt
    39961 aggttctctg cacactcttg gcgggttgca atatcttggt ctggaattac cggaagatcg
    40021 ctatggcgcg attcttcgtt atcaaaccga ccatgacgaa gccgggcgtg caacctcgtg
    40081 tgggccgcgt acctcaaggt taatggtgaa agtgctgctg gaagaagtac agcgtcttgc
    40141 tatcccaatg ttgaccagtg caacagtgat taaactgctg catcagcgtg acgaaaacgg
    40201 tgaagaccgt gtggcggggg caatcctcgc gaccggtcat cgcgcccata acccatgggg
    40261 gctggcaatt gtgactgcgc ccaatgtggt actggcaaca ggagggcctg gcgagcttta
    40321 tcgcgacagt gtgtatccac acaaatgttt cggctcgctg gggctggcgc tggaggaagg
    40381 tctgacgcta accaatctga ctgaaagcca gtttggtatc ggcacaccgc gcagcacgtt
    40441 tccgtggaat ttgtccggga cttatgtaca ggtgatccca tatatctatt ccgtggatgc
    40501 cgggggtaac gagtataact tcctcgcgga ttactatcgc accacccagg agctggcttc
    40561 aaacattttc cgtaaaggct atcagtggcc gttccacgcc acacgggtaa tggattttgg
    40621 ctccagcttg ttagatatgg cagtagcgca agagcagcaa tcagggcgtc aggtatttat
    40681 ggatttcaat cgcaatcctg aagcggtgcc gggtgacctg ccgttctcat tagatcgact
    40741 ggacgacgac gttcgcgcgt atctggaaaa taacgacgct ctggcaccat cgcccatcga
    40801 acgcctgcaa cgaatgaatc cgctgtctat ctcgctgtat aagatgcacg gttacgatct
    40861 caccacgcag ccattgcagt ttgccatgaa taatcagcac atgaatggcg gcattgaagt
    40921 cgatatctgg ggacaaacat ctctgcccgg ttgttttgca gtgggggaag ttgctggcac
    40981 acacggcgtc acccgacctg gcggcgcggc gttgaatgcc gggcaggttt ttgctgttcg
    41041 tctggcgcgc tttattggtt gcacgcaaaa gcggaatatt gatggagata ttgcacagct
    41101 ggcagctccg gcactggctt ctataagaga gatcattact caggcgcacg ataacgggac
    41161 cgggatgccg ttgtcggttg tgagagaaaa aattcaggca cgaatgtctg accatgcggg
    41221 atttatttgc catgccgata aagtccgacg cgccactcgt gatgccctgc tattgagtga
    41281 atttgtgcaa cggcatggat tggcaatcaa acatgtgggt gaagttgccg agctgtttat
    41341 gtggcggcat atggcgctga cttctgccgc ggtattaaca caattaacgc attatattga
    41401 tgctggtggt ggtagccgtg gggcgcgtat ggttattgat ccacaaggca aatgcctacc
    41461 acaaactcgt cgcggcgcaa aagaagaatg gcgttttcgc tctgaactcg ctgaagacaa
    41521 aaatcacaaa ttaacaattc aatattcgca aggttctttt attaccgaag taaagtcttt
    41581 acgtatgcaa ccgtgtatta acggtattta ctttgaaaaa aactggccag actttttaaa
    41641 gggagacact tacacacaat aatt
//
LOCUS       DADSZD010000020        40787 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.020, whole genome shotgun sequence.
ACCESSION   DADSZD010000020 DADSZD010000000
VERSION     DADSZD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.020"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(795..1832)
                     /locus_tag="J1Z28_000968"
     CDS             complement(795..1832)
                     /locus_tag="J1Z28_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HBA7715824.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRVREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGVGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            complement(1885..1979)
                     /locus_tag="J1Z28_000969"
     tRNA            complement(1885..1979)
                     /locus_tag="J1Z28_000969"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1943..1945),aa:Sec,seq:tca)
     gene            2272..3654
                     /locus_tag="J1Z28_000970"
     CDS             2272..3654
                     /locus_tag="J1Z28_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBA7715825.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3664..5982
                     /gene="yicI"
                     /locus_tag="J1Z28_000971"
     CDS             3664..5982
                     /gene="yicI"
                     /locus_tag="J1Z28_000971"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBA7715826.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGAEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPDADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     IVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            6277..7830
                     /locus_tag="J1Z28_000972"
     CDS             6277..7830
                     /locus_tag="J1Z28_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBA7715827.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEEQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            7857..8327
                     /locus_tag="J1Z28_000973"
     CDS             7857..8327
                     /locus_tag="J1Z28_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017801221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HBA7715828.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            8345..8659
                     /locus_tag="J1Z28_000974"
     CDS             8345..8659
                     /locus_tag="J1Z28_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBA7715829.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADISAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            8684..9775
                     /locus_tag="J1Z28_000975"
     CDS             8684..9775
                     /locus_tag="J1Z28_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009112672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA7715830.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            9787..10638
                     /locus_tag="J1Z28_000976"
     CDS             9787..10638
                     /locus_tag="J1Z28_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HBA7715831.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVALALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEISRCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            10719..11579
                     /locus_tag="J1Z28_000977"
     CDS             10719..11579
                     /locus_tag="J1Z28_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBA7715832.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFSHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMDILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILVKESWWDPNVIYPDAINAAREVIRHKMKL
                     FGSLGKASLY"
     gene            11627..12559
                     /locus_tag="J1Z28_000978"
     CDS             11627..12559
                     /locus_tag="J1Z28_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBA7715833.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            12540..13220
                     /locus_tag="J1Z28_000979"
     CDS             12540..13220
                     /locus_tag="J1Z28_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HBA7715834.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CGEYGILR"
     gene            complement(13264..14973)
                     /locus_tag="J1Z28_000980"
     CDS             complement(13264..14973)
                     /locus_tag="J1Z28_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBA7715835.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQVNVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(15094..16485)
                     /gene="xanP"
                     /locus_tag="J1Z28_000981"
     CDS             complement(15094..16485)
                     /gene="xanP"
                     /locus_tag="J1Z28_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBA7715836.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16765..17970
                     /gene="gltS"
                     /locus_tag="J1Z28_000982"
     CDS             16765..17970
                     /gene="gltS"
                     /locus_tag="J1Z28_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBA7715837.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(18004..20085)
                     /gene="recG"
                     /locus_tag="J1Z28_000983"
     CDS             complement(18004..20085)
                     /gene="recG"
                     /locus_tag="J1Z28_000983"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBA7715838.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRIDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(20091..20780)
                     /gene="trmH"
                     /locus_tag="J1Z28_000984"
     CDS             complement(20091..20780)
                     /gene="trmH"
                     /locus_tag="J1Z28_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBA7715839.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(20787..22895)
                     /gene="spoT"
                     /locus_tag="J1Z28_000985"
     CDS             complement(20787..22895)
                     /gene="spoT"
                     /locus_tag="J1Z28_000985"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBA7715840.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(22914..23189)
                     /gene="rpoZ"
                     /locus_tag="J1Z28_000986"
     CDS             complement(22914..23189)
                     /gene="rpoZ"
                     /locus_tag="J1Z28_000986"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBA7715841.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(23244..23867)
                     /gene="gmk"
                     /locus_tag="J1Z28_000987"
     CDS             complement(23244..23867)
                     /gene="gmk"
                     /locus_tag="J1Z28_000987"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBA7715842.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            24125..25807
                     /gene="ligB"
                     /locus_tag="J1Z28_000988"
     CDS             24125..25807
                     /gene="ligB"
                     /locus_tag="J1Z28_000988"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBA7715843.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGIYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLKRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNAGWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRTLHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(25804..26421)
                     /locus_tag="J1Z28_000989"
     CDS             complement(25804..26421)
                     /locus_tag="J1Z28_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBA7715844.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(26713..27537)
                     /gene="dinD"
                     /locus_tag="J1Z28_000990"
     CDS             complement(26713..27537)
                     /gene="dinD"
                     /locus_tag="J1Z28_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBA7715845.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AVGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(27758..28621)
                     /locus_tag="J1Z28_000991"
     CDS             complement(27758..28621)
                     /locus_tag="J1Z28_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBA7715846.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            28748..29464
                     /gene="rph"
                     /locus_tag="J1Z28_000992"
     CDS             28748..29464
                     /gene="rph"
                     /locus_tag="J1Z28_000992"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBA7715847.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            29530..30171
                     /gene="pyrE"
                     /locus_tag="J1Z28_000993"
     CDS             29530..30171
                     /gene="pyrE"
                     /locus_tag="J1Z28_000993"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBA7715848.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(30208..30804)
                     /gene="slmA"
                     /locus_tag="J1Z28_000994"
     CDS             complement(30208..30804)
                     /gene="slmA"
                     /locus_tag="J1Z28_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBA7715849.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QLLAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(30911..31369)
                     /gene="dut"
                     /locus_tag="J1Z28_000995"
     CDS             complement(30911..31369)
                     /gene="dut"
                     /locus_tag="J1Z28_000995"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBA7715850.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(31347..32567)
                     /gene="coaBC"
                     /locus_tag="J1Z28_000996"
     CDS             complement(31347..32567)
                     /gene="coaBC"
                     /locus_tag="J1Z28_000996"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBA7715851.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKALLGQLL
                     LDEIVTRYDEKNRR"
     gene            32739..33407
                     /locus_tag="J1Z28_000997"
     CDS             32739..33407
                     /locus_tag="J1Z28_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HBA7715852.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            33624..33860
                     /gene="rpmB"
                     /locus_tag="J1Z28_000998"
     CDS             33624..33860
                     /gene="rpmB"
                     /locus_tag="J1Z28_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBA7715853.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            33881..34048
                     /gene="rpmG"
                     /locus_tag="J1Z28_000999"
     CDS             33881..34048
                     /gene="rpmG"
                     /locus_tag="J1Z28_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBA7715854.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            34146..34955
                     /gene="mutM"
                     /locus_tag="J1Z28_001000"
     CDS             34146..34955
                     /gene="mutM"
                     /locus_tag="J1Z28_001000"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBA7715855.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(34994..35473)
                     /gene="coaD"
                     /locus_tag="J1Z28_001001"
     CDS             complement(34994..35473)
                     /gene="coaD"
                     /locus_tag="J1Z28_001001"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBA7715856.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(35481..36758)
                     /gene="waaA"
                     /locus_tag="J1Z28_001002"
     CDS             complement(35481..36758)
                     /gene="waaA"
                     /locus_tag="J1Z28_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HBA7715857.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVSLGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATSLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            37171..38229
                     /gene="rfaQ"
                     /locus_tag="J1Z28_001003"
     CDS             37171..38229
                     /gene="rfaQ"
                     /locus_tag="J1Z28_001003"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBA7715858.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKITNFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNVRVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            38226..39350
                     /locus_tag="J1Z28_001004"
     CDS             38226..39350
                     /locus_tag="J1Z28_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA7715859.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAVADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            39343..40140
                     /gene="rfaP"
                     /locus_tag="J1Z28_001005"
     CDS             39343..40140
                     /gene="rfaP"
                     /locus_tag="J1Z28_001005"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBA7715860.1"
                     /translation="MVELKEPFATLWRGKDPFGEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWAINPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSSKEEELKISVIDLHRAQIRTCVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEEKATKIRERTIRKSL"
     gene            40156..>40787
                     /locus_tag="J1Z28_001006"
     CDS             40156..>40787
                     /locus_tag="J1Z28_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HBA7715861.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPEASVASSFHVAYGIDQNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPDADIQRLSQLAKSYHTCIQIHLVNCERLKVLP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGTLKPLITMDLANNVAAVVTER
                     DANWWSLRAQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKA"
BASE COUNT         9802 a         9890 c        10776 g        10319 t
ORIGIN      
        1 tcgcagttca attttattat cgttttcgaa taaataaagg gcattattgt tttgaaacag
       61 attgaatata ttgttagcgc aagtttgagt tgctagccag gatgccgctg gatgactcaa
      121 attaatatgt gtgctaaaga ctttgacgcg actacttctg tttttctctt tactttcaat
      181 taaatgccta gcgtttttgg ctgctttaca agttccatgc cccacacggc aaaaataatc
      241 tcctgaaagg atacttacaa tcagtattac accaatgacg acagaaaaaa tagttccgat
      301 gagtttgatg atgacaatcc ttgtgcagtg ttattttcac catggattat aatgttatag
      361 tgaaattatt gcgcatgtaa tttttctctg attgttttaa catcacattt atgcacttct
      421 tcatctttgt agcaggtaaa agccatatat ggatggctgg tgtccggtgc agataaaatg
      481 caacttattc ttgatagctt gaaaaaaaga gaggtttttt tgagtgacgg ttgatagtcg
      541 gtggcaagga agacgttttc gtacactcgg caagaaaaac gaatcactct gtcatactac
      601 cagatacatc agcagggtca ggttgctcag aaataagcag gatgaggatc actgaagagg
      661 cattttggcg atcacaggag tcgcatttga tggttaagtt attgatttaa tgtttatatt
      721 ttgaatttga taaatgagta accccataag tacccccaaa tggttttgcg tatggcgttt
      781 ggggggattc cgggctactt tttcgactct ttattccact gtagcaaacc agcgatatcc
      841 ttctcgcctt cggcttcttt taagcgccgt tgttgttcgg caaactgaac atactccgcc
      901 tgcgcttttt catcggccat tttcttgctg attttaccaa cgccctgtag aacctctcgg
      961 tcgttaaatt gcaggaactg atccagctta tcctgccagt cgcgcaaaaa gacttgctga
     1021 cgacgacggg cctgatcctc ggcaaagtcc agccacatat taaccacgcg attaagttcg
     1081 ctgacctcat cctgggtgag ataatttttt gccaccgtca cgtcgccctt acgtacctct
     1141 tcacctttat aactggtcaa gcccatgtgc ggctggctgg catctgctcg tttgtggatg
     1201 agttccgcag cggtatgccc ggtacaggca aaatgcagtt tattctggat ggtttgaaaa
     1261 aattgcgtgg tttctttaag cgatggctga tagtcggcag ctaaagcaaa gatctcccgc
     1321 acccgcaaat atacccgacg ctcgctggcg cgaatatcgc ggatgcgttc aagcatctca
     1381 tcaaaatagt cgggcacaac cgatgaaccg acaggcggat tcttcagccg ctcatcgtcc
     1441 atcacaaaac ctttgatcag gtattcctga agcgtctggg tggcccactg gcggaattgc
     1501 gtgcctcgag gcgagcgaac gcgatagccg acagcgagaa tggcttcgag gttgtaatgg
     1561 tctatctctc tggaaacctg acgttttcct tcctggcgaa ctatccggaa tttccggatg
     1621 gttgagtttt gaacaagttc accttcggaa aatatattga ttaaatgttc gttaatagtg
     1681 cgaacatctt tatcgtacag ttcagccatt gccgcctgag ataaccagat tgtgtctgac
     1741 tcaaagcgac attcaacacg cactcttccg tcaccgctgg caaacataat aaactcgcct
     1801 gctgggggct gggttaagtc tttgtctgac atgtcacctc cgaaggagtg ttgttgacgt
     1861 cgggagagtt cagtagaggc attttggcgg aagatcacag gagtcgaacc tgcccgggac
     1921 cgctggcggc cccaactgga tttgaagtcc agccgcctca ccggagacga cgatcttccg
     1981 cgcctcgatt gctacatgga ggcggggcgc attatagcta cttccttgag tttctacatc
     2041 ccccagatca gttttttccg cctaatacac gcagcgtatt atcacgaaaa cattatagtt
     2101 catataaaac tcatatttta caatgatata tgagcgaaat taacgctacc acgatcacat
     2161 aaaacaagaa tcataaatta ataaccagat atcggaatat tcgctctcgc agggatggtt
     2221 tacaaaatgc gttatcggtc tcagcacccc tatagcatca aggaaaagca gatgaagagt
     2281 gaagtgttgt ccgttaaaga gaaaattggt tatggcatgg gagacgccgc cagccacatt
     2341 attttcgata acgtaatgtt atatatgatg ttcttttata ccgatatttt tggcattcct
     2401 gccggatttg tcggaaccat gtttttggtc gctcgtgcac tggatgcgat ttccgatcct
     2461 tgcatggggt tgttggccga tcgaacgcgc tctcgctggg gtaaatttcg tccgtgggta
     2521 ctgtttggcg cactgccatt cgggatcgtc tgtgtactgg cctatagcac gccagatctc
     2581 agtatgaacg gaaaaatgat ttatgcagca attacttaca ctctacttac cttactttat
     2641 accatcgtca atatccctta ctgcgcattg ggtggtgtaa tcaccaatga cccgactcag
     2701 cgtatctcgc tgcaatcctg gcgttttgtg ctggcgaccg cgggaggcat gctctctact
     2761 gttctgatga tgccactggt taatttaatt ggtggtgata ataaaccact cggtttccag
     2821 ggcggtatcg cggtcctttc cgtggtggca tttatgatgc tggcattctg tttcttcacc
     2881 actaaagaac gcgttgaagc accacctaca acgacgtcta tgcgggatga tttacgtgat
     2941 atctggcaaa acgaccagtg gcgtattgtc ggtttcctga caattttcaa tatcctggcg
     3001 gtgtgcgtac gcggtggggc gatgatgtat tacgtcacat ggattttggg tacgccggaa
     3061 gtgtttgtcg cttttctcac cacttattgc gtgggtaacc tgattggctc cgcactggcg
     3121 aaaccgctga ccgactggaa atgtaaagtc actatcttct ggtggacgaa cgccctgctg
     3181 gcagtgatta gcctggcgat gttctttgtt cctatgcagg ccagcatcac catgtttgtc
     3241 ttcatcttcg tgattggtgt gctgcatcaa ctggtgacac ctatccagtg ggtaatgatg
     3301 tccgataccg tcgactacgg cgagtggtgc aatggtaaac gcctgaccgg gatcagtttt
     3361 gctggcacgc tgtttgtgct caaactgggg ttggccttcg gtggtgccct tatcggctgg
     3421 atgctggctt atggcggata tgatgcggca gaaaaagcgc agaacagcgc cacaattagc
     3481 atcattatcg cgctattcac gattgttccg gcgatctgtt acttgctgag cgcgattatc
     3541 gctaaacgct actactcgct cacgacgcac aatctgaaaa ccgttatgga acagctggcg
     3601 cagggcaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa ctaaggaacg
     3661 gcaatgaaaa ttagcgacgg aaactggttg attcaacctg gcctcaattt gattcacccg
     3721 cttcaggtgt tcgaggttga acagcagggt aatgaaatgg tggtctatgc tgcccctcgt
     3781 gatgtgcgtg aacgtacctg gcagcttgat actcctttat ttacgctgcg ctttttctcc
     3841 ccacaggaag gtattgtcgg tgtacggatt gagcattttc agggggcgct gaataacggt
     3901 cctcattacc cgctcaatat tttgcaggac gtgaaggtca ctatcgaaaa cacagagcgt
     3961 tacgctgagt ttaaaagtgg caacttaagt gcgcgtgtca gtaaaggtga gttctggtca
     4021 ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa taacggctac
     4081 gtgcaggaca cgaataatca acgaaattat atgtttgagc ggctggatct tggcgttggc
     4141 gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg ccagacggta
     4201 gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa tatcccgttt
     4261 tatatgacta accgtggtta tggggtactg gtcaatcatc ctcaatgcgt ctcttttgaa
     4321 gtgggagcag agaaagtctc caaagtacag ttcagcgttg agagtgaata tctcgaatac
     4381 tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg ttttactggc
     4441 cgtccggcgc tgccgcccgc gtggtccttc ggtctgtggc taaccacttc atttaccacc
     4501 aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg caatctgccg
     4561 ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg cgattttgag
     4621 tgggacccgc tgacttttcc ggacccggag gggatgatcc gccgtctgaa agcgaaaggg
     4681 ctgaaaatct gcgtctggat taacccctat attgggcaaa aatcccccgt ctttaaagag
     4741 ttacaagaga aaggttattt actcaaacgc ccggacggtt cgctgtggca gtgggataaa
     4801 tggcagccag gtctggcgat ttatgacttt accaatccgg atgcctgcaa atggtacgcc
     4861 gacaaactga aaggtctggt cgcgatgggc gttgattgct ttaagaccga ctttggcgaa
     4921 cgtattccaa ccgatgttca gtggtttgat ggttccgatc cgcagaaaat gcataaccat
     4981 tatgcgtaca tctacaacga actggtgtgg aacgtgctca aggacaccgt tggtgaggaa
     5041 gaagccgtct tgtttgcccg ctcggcctcc gttggtgcgc agaaatttcc ggtacactgg
     5101 ggtggcgact gttacgctaa ctacgaatca atggcggaaa gcctgcgcgg tggtttgtct
     5161 attggccttt caggttttgg cttctggagc cacgatatcg gcggctttga aaataccgct
     5221 ccggcgcacg tttacaaacg ctggtgcgcg tttggtttgc tttccagcca tagccgttta
     5281 catggcagca aatcttatcg tgtgccgtgg gcctacgatg atgagtcctg tgatgtggtg
     5341 cgcttcttca cgcaactgaa atgccgcatg atgccgtatc tgtatcgtga agctgcacgt
     5401 gcaaacgccc ggggtacgcc gatgatgcgg gccatgatga tggagttccc ggacgatccg
     5461 gcttgtgatt accttgaccg tcaatatatg ttaggcgaca acgtgatggt tgcgcctgtg
     5521 ttcacagaag cgggcgatgt gcagttctac ctgccggaag gtcgctggac acacctgtgg
     5581 cacaacgatg aactcgacgg tagtcgctgg cataaacagc agcacagctt cctgagtctg
     5641 cccgtttatg tgcgtgatag caccctactg gcgctgggca acaacgatca acgtcccgat
     5701 tacgcgtggc acgaaggtac ggcattccac ctctttaatc tgcaagacgg gcatgaagcc
     5761 gtctgtgaag tgcccgatgc tgacggttcg gtgatcttta ctttaaaagc agcacgtact
     5821 ggcaacacga ttactgtgac tggtacgggc gaggcgaaga actggacact gtgcttgcgc
     5881 aatattgtga aagtaaatgg tctgcaaggc ggttcgcagg ctgaaagtga gcagggactg
     5941 gtggtgactc ctcaagggaa tgcgctgaca attacgttgt aatctattga agaatgccgg
     6001 atacgaatgg ttttgtgtcg tatccggtaa ataatctcac atctggtaat aaatatttct
     6061 tccatcgtat ttgcgagcat gatcacgatt attcatcacg aaaatgtgat ctgtgctgaa
     6121 aaatatccct taaccgcctt gctttgtttt atcactgtcc ggattacgga cagattttag
     6181 tattgttgtg aaattattct gctttatatt taccctcaga gatggcaact gaagctcttc
     6241 catatggggc ttcttaatct gtgataaggg taaaaaatgc aaatgattac ctcgcgacaa
     6301 aacagattat tacgattcct tttacctcga agggaatata cgactattgt tacgattgcc
     6361 ggttatttaa atgtttcgga aaaaaccatt caacgtgatt tacgtttact tgagcaatgg
     6421 ctgggacaat ggagaataaa tgttgagaag cgtgctggcg cgggtgtgat gttaagtgcg
     6481 gagaatattg ctgatttgct gcatcttgat catttgttgg tggcagaatg tgaagagatt
     6541 gatggtgtaa tgaataatgc caggcgtgtt aaaatagcgt cgcagttatt aagtgaaaca
     6601 ccgaatgaaa cgtcgatcag taaattgtca gaacgctact ttatcagcgg agcctctatt
     6661 gttaatgacc tgagagtaat tgagtcctgg cttgcgccgt tggggttatc gttgatccgc
     6721 agtccaagtg gtacgcatat tgaaggtagt gaagggcaag tccgacaggc aatggcatta
     6781 ctgattaacg gcattattaa ccataatgag ccgcaaggtg tcgtgtattc acgtctggat
     6841 cccggaagct ataaagcatt agtccattat tttggagaag aagaggtgtt atttgtccag
     6901 tcattgttac tggatatgga aaatgaatta agttggtctt tgggagaacc ttattacgtt
     6961 aacattttta ctcatatcct tattatgatg tatcgcaaca cgcacgggaa tgcgttatca
     7021 agagaagaag aacaaaccag gcaatatgat gaaaatatct ttaatgttgc cagtcagatg
     7081 attcataaga tagaacaacg aattgcacat acattgcccg atgatgaagt ctggtttatt
     7141 tatcaatata tcatttcatc aggtgtggcg attgatggac aaaaagatgt tagcattatt
     7201 tcacatatgc aggccagcaa tgaagcgcgt ctgattacct ggcgtttaat tacggtattc
     7261 agtgacatcg tggactgcga ttttagtgaa gacagcgcat tatatgatgg cttattggtg
     7321 catattaagc cgctgattaa ccgtctaaat tatcgcattc atatccgtaa tccattgctg
     7381 gaagatatta aagcagaatt agcggatgtc tggcggttga cgcaatatgt ggtgaatcag
     7441 gtatttaaaa cctggggtga gaatgcagtg agcgaggatg aagtgggtta cctgaccgtt
     7501 cattttcagg ctgcgatgga gcggcaaatt gcccgtaaac gtgtattact ggtctgttca
     7561 accggaatcg gaacttcgca tctactgaaa agccgtattc tgcgagcatt tcctgaatgg
     7621 acgattgttg atgttatttc agcagcgaat ttatcacagg ttttgcctga caatatcgaa
     7681 ctgattattt cgaccattaa tttgcctaca gtcactatgc cggtcgctta tgttaccgct
     7741 ttttttaatg atgccgatat taagcgggtc actgaaatgg tgattacgga aaaattacat
     7801 catgcgacgt ctcgggtcgt tgaaatttaa tgtcagaatt ttatgaggta tgacgaatgg
     7861 acataacaaa aatcctgaat acgaatcgcg ttattttaga tatgcaagca acgaataaag
     7921 cggaggcgat tgaagaatta actaatcttc tcaaaaaaga tggtgcgatc gattgtcggg
     7981 aaacctttat taaagatgtc tggcagcgcg aagcggaagg ctcgactggt tttgaaaatc
     8041 atattgcgat cccgcatggt aaatcttctg cggtaattaa tactacgctg gcaattggcc
     8101 gtacccgcca ggatattccc tgggaaacgc tggatggcag tagcgtgcgc tgtattattt
     8161 tatttgcggt acgtcttgaa gatcaaaata ccacccatat ccgtttactt tcccaggtcg
     8221 cgggcgcgct ggcggatgat gacatcatag aacaattgtt agttgagacg agtccgcaaa
     8281 aaatcattga tctctttagt cagtatgcac aatccgatgt ctgctaaata acaggaagta
     8341 tgtcatgaat attgtctgtg ttgccgcctg tacggcgggt attgctcata cctatattgc
     8401 tcgtgaaaag ttaatcaaag gagcaaaagc tctgggccat accattaaag ttgaaaccca
     8461 gggaaccatt ggtacagaaa atgaattaca ggcggcagat atctctgctg ctgacgtcgt
     8521 gattcttgct gtagatgtga aaataaaagg agaagaacgc ttcaccaata aacgtattgt
     8581 gcgagtaaag acagagatcg tcattaaatc gcccgttcaa tttcttgaaa aggtcgaaaa
     8641 atcactggga aacaaatgat ccttatactg gagggatctt aatatgaaag aaaataaaat
     8701 tccggtttca caggaaatta aaaaacattt attaaccggc atctcgtgga tgatacccct
     8761 tattgtggcc gccgggatat gtattgcgtt ggggcaagtt cttggcggta cagatgttgg
     8821 tgaaaaaaca ggaacaatcc cgtggatgct taatcagatt ggcggctggg gaatggggct
     8881 gattgtgccg ctgattagcg cggcaattgc ctattctatt gccgatcgtc ccggatttgc
     8941 cccaggcctg attgtcggct ttctctgcgg gcaaatccat accggattta ttggcggtat
     9001 gttgggtggt ttcctggtgg gctacaccat tttgctactt aaacgctata tccgcctgcc
     9061 gcagtcgatg caaggactca tgccaatcat ggtgttgccg gtattaagca ccattatcgg
     9121 tgggctgtta atgatgacgc tgattggtaa acccatcgcc tggttacaag aagcactgat
     9181 tcatttgctc gagtcgatgc agggcggatc gcgcttcctg atgggggcca tacttggcgc
     9241 gatggcaacc ttcgatttcg gtggtccggt caacaaaacc atgtcgctgt tttccgatgg
     9301 cttgctggtc agtggcgtct acggaccaga agccgtgaaa tttgtcggct caatcatccc
     9361 tccgtttggc atcacacttt cgttcttact gacacgccat aaatacaccc gtgcggagcg
     9421 cgaggcgctg aaagccgcgt ttccgatggg gatctgcatg atcactgaag gagtcattcc
     9481 gatcgccgcg cgtgacctgc tgcgagtagt gggatcttgt gtggtggcat cggcggtcgc
     9541 gggcggctta atcatggtgt ggggcgtgga gagtccggtt ccgcatggcg gcatgtttgt
     9601 tgtgcctctg ttcacccatc ctctgctgtt ttgcctggcg ctgggtatag gcacggtgat
     9661 ttgtggcgtg atgctttcac tctggaagaa accggtaacg gaacgcgacg aagagtttga
     9721 cgaactgagc gatcaaaagc tgaaagatga agagatcact ttcaccctcg aataaccgga
     9781 ggccctatgt ttgccgatat gaaaagtatg gtgatgaaag cctggcatga acattacgcc
     9841 ttgctggcga tcaactgtat gaatctggaa agtgctcacg cagctatccg cgtggcggag
     9901 aagaatcgcg ctccgatcat tctcaatctc tatcaggggc atctggcgca ttttcctgca
     9961 cccgtcgcgg cggcagtggt taaaacgctc gcggaatctg cctcagtacc ggtcgctctg
    10021 gcgcttgatc atggcaaaaa tccggactgt attcgtcagg cgtttcgtgc tggtttcagc
    10081 ggattaatga ttgatgcttc tgcttttccc ctggaggaaa acgtgcgcca gaccagggcg
    10141 gtggtggaac tcgccgccag cgcgcagctc tgcgtggaag gcgaactggg ccatctggca
    10201 gatgcgccgc gttacgacca ggccgccaat gcagacctga tgacgcaacc cactgatgtg
    10261 gaaccgttta tcgcgcagac aggcattgat ctactggccg tctcggtagg cacggcgcac
    10321 ggcatgtacg cgcctggcgt tgtacctgct attgattttc agcgtctggc ggagatttcg
    10381 cgctgctcga gcgtgcctct ggcattacat ggcggtagcg gtacgccgtt cgatcagctc
    10441 cagctctgta cgacgctggg ggtagcaaaa atcaacgtgg gggcggccat ttttgaacgg
    10501 gggaaatccg cgctcctgca taccctctgt cacgatatat ccattgaact ggtggacgcg
    10561 ctgaaagcta tggaactggc cttcgaagaa gcgattacac cgtatttaca ggcgagcggc
    10621 tctatcggca aagcctgatt cattttctgt catggccttt atgccagtgc aataacgcac
    10681 cggcagggcg gactacacgc tgaaactgga gatttaacat gttagtttcg atgaaagaat
    10741 tactgcaacc tacccgccag cacggctttg ccattggcgc atttaatgtg gcggataact
    10801 gtttccttcg cgcagtcgtg gatgaagcag aagcgacaaa tactccggcg attattgcca
    10861 tccatcccag cgaacatgat tttgtgggcg atgccttttt ctctcacgtt cgcgaaatta
    10921 cccagcgtag tccggtcccg tttgtactgc acctcgatca tggtgcctct gtagagcatg
    10981 ttttgcgtgc gattcgctgc ggatttacct cagtaatgat cgacggttcg ctgctgccgt
    11041 atgaagaaaa cgtggcgctg accgcagaag tggttcgcct cgctcacgcg gttggagtat
    11101 cggttgaggg tgaactggga accatcggac aaacaggaac gtcggtagaa ggtggtgttt
    11161 cacaagtgac ctacacagat ccggcgcagg ccgccgattt tattgctcgt acaggtgccg
    11221 acacactggc tgtggcgatt ggtacagcgc atggcatcta tccaaaaggg atgcagccga
    11281 agctgcaaat ggacattttg cgcgacattg ccggacgtat tgatattccg ctggtgctac
    11341 acggtggttc ggcaaacccg gacgcggaga ttgctgaatc agttacgctc ggcgtcggca
    11401 aaatcaatat ctccagcgat atgaaatacg cctatttcca gaaagtgcgt gaaattctcg
    11461 ttaaagagag ctggtgggac ccgaacgtca tctatccgga tgcgattaac gcggcgcggg
    11521 aagtcattcg ccacaaaatg aaactgttcg gttcgctcgg taaagcatca ctgtactaag
    11581 ccagaagggc ggggtgaccc gccttttacg caacaaggaa cgagatatgc actatctggg
    11641 gttggatatt ggtgggacca aaatcgccgc cgttgtcatg gatgcgcatg gctgggagat
    11701 tcgccgttac cgctgcccga cgcaaaagtc gacatatcaa caatttgtct catgcgttgt
    11761 ggcgcttatc gagcagatta ggcgggacgt tcaacgaccg atgctgacag ggatcgcctt
    11821 acccggcagt atctcgccac tcactggcct gattaaaaac gcgaatattc aggtgattaa
    11881 cggtcatgcg ttacaggctg atttgcagca attgcttggg caaccggtgg tgatagccaa
    11941 tgatggtaac tgtttcgcgc tatcagaagc ttgcgacggt gccgggcaag attatgacgt
    12001 ggtatttggt attacgcttg gtacgggctg cggcggtggc attgccatca agcaacgacc
    12061 gtttataggg gcctggggaa atgctgccga atgcggtcat atcacgttgc caggctatac
    12121 ggagcaggaa gatggtccat cagtcagttg ctattgcggc aaacacaact gcgtggagtc
    12181 gtttgtttcc ggcagcggtt ttagtgaacg ctatcaacag atgactggta acttgctcac
    12241 tcctgcggcg attgtcaccc tggcacaacg tggtgatgct tgtgccatgc agcaggtggc
    12301 acgttttcgc caacagcttg cccgcacgct ggcaaccatc gttaacgttg ttgaccctgg
    12361 cgtgattgtg atcggcggcg ggctttcgaa tgtggaactg cttatcaccg atctgaacgc
    12421 agaagtcgct ccactggttt tcaccgatca attcaccact cccattgtaa aagcacagca
    12481 cggcgacagt agcggaatgc gtggcgctgc ctggcttgct atgcgcaacg gagaagccaa
    12541 tgaaacgttc acaaattaat tatgttattg ataaagcaca tgccattgcg cagaccttcc
    12601 gcgtttgttt gccagagttc gcctatttta cggtggatga ctggctgcag cgggaacggg
    12661 ataactggca agaagtggtc gacttacagc tcggttggga tatcaccgat tttggtcgtg
    12721 gtgatttcaa ccaaacggga ctaacactgc taacgatgcg caacggcgcg ctggggtcgg
    12781 cggcatatcc aaaaccgtat gctgagaaaa tgttgcagat ccaacaagat cagcagactc
    12841 cctggcattt tcacacccac aaaatggaag atatcctcaa tcggggcggt ggagacttat
    12901 gcatgcagtt ggcctgggcc aacgaagcca atctctgtga tgatcaacga gtcattaccg
    12961 tgagtgttga tggtcagcgg cgtaccatga aacctggtga aacgctggtg ctaaagccgg
    13021 ggcagggcat ttgcctgcca ccgcgtctgt atcatcgctt ctgggcggaa aaagcgtttg
    13081 ttctcggctg ggaaatctcg atggtaaatg atgaccaaca cgataattat tttcttgagc
    13141 cgggaggacg ttttccggcg atagaagaag atgaaccggt gaaatggtta ttgtgcggtg
    13201 agtatggaat tttgcgctaa taacggggaa cgctagcgct gatatccgct agcgtcctta
    13261 tttttactgc accggctccg tcgacgtaac ttcaccactg gaaactacgc ccgcattcat
    13321 ggtttgagtc acttgctgct ttgcggcatt cacggcatgc aattgcccgc taaccgtagg
    13381 tttcagcggg acattgacct gaatatcgcc actggcggta agctgaatat tgccgtcgcc
    13441 agtcaacggt aattcaggcc atccccaatg ttgcaaaata ttcaccggca caccgcgtcc
    13501 attcaggctg atatgggtct gacgttgtgg cgtttgtgaa acactggcgg tggcttccag
    13561 aatgcctttt tcagtaaatg cactcagttc gctgatattc accgtgctgc tgttggcagt
    13621 tagcgccagc gacggacgac ggacatcaac acgattaaac gttgcggcgg cggcattcag
    13681 attcgccgag ccactccaga cgccccattt atgatcggta accagcgtca gattggcacc
    13741 gtaaccatcg agcgcggtga gctgccacgg gaagtcaggg tcgatatcaa tgatcagatt
    13801 gcggctggcg ctaaatctct tcagttgcag gctgtttaac caaccgggtg tcgtttccat
    13861 ccacaactgt tgccagtttt tgggcaaggt atattccagt ccggcaattg ccgcatcatc
    13921 gaggatcagc gttttcccgt cgcgttgcca gtttcctgac gttctgacca tacccccttc
    13981 ccagcggctg gtgaactggc gcagtgctac gccctgcggg gaaaattccg cgtttataat
    14041 cggatcaaat aaatgcagcg agccataaat aaactcgctg gcgttcatcg acagtttacc
    14101 ttcctgtgtc tgccagtcat ctttactgaa ggtcatgttg cgcaagctga gatcgaggtc
    14161 ggtcaccgcc cagtccggac cttgcaaacg agcgtcgatg acttccaggc gaccaatctg
    14221 caacgacggg acagagcgta atggcgcaaa gaagtcggtt agcgattttt cgctttgtag
    14281 acggatatcc gccatgcgca ggttttccac ttgccagctg ccgtcagcgt tacgctgcgc
    14341 gtttccggtt aatgtcccgc gggcgatgtc ggcacccagg ttagtcagcg taacgcgatc
    14401 gttatcaata ctgccttcaa tcagtacatt ggtggcagga acatcattga gcgaaagcga
    14461 tccggcacta aactgaatct gcgccttcgt acccagcact ttaccggctt ctggtgacca
    14521 cggaaccacg ccgccattta cccgctgcgc gctcagtttc cattcgctat tcgggctatt
    14581 aaacgccata tcacgcagtt gcagacgatc ggctttgaac ggtagcggcg cggtctggtc
    14641 ggtgagattc agcgtcccgt tttccagcag gatggtatcg acatggcgtg gttcggtcag
    14701 ttgccgactg cttagcgcaa tgtcgacact ttttgccacc agggtcgcgg gctggccatc
    14761 acggccaaac gtgacgttct ccagcacgat atgagatggc gcggaaaaac ggtgatccat
    14821 cgccccgaag gccagatgat agtcgctatt ctcggaaacc catgcgctga tatgttctgc
    14881 tccccagcgg gtttgcagaa gaaaatagag gccagcgatc accactaaca gagcgatgag
    14941 aatgtagaga agcagcttcc caataaattt catggtcttc catcccgtga aatgcacata
    15001 agggagttat gcacgattta cgcgcaatcc tcaaggcggg aatggtgaaa gaggtgagac
    15061 tgcggcggta attatcttac cgccgccagt gaattactgt ttttctgggg ggaaaatcag
    15121 attcagcacg atggcagtaa taccgcccgc ggcaatcccg gaggagagca ggtttttcag
    15181 ccattcaggg gcaaactgca aaatcagcgg ctgctgagac acgcccagac cgaccgccag
    15241 cgacagcgcg ataatcagaa tcgcccggcg gttcagcggc tcacgagaaa cgatacgcac
    15301 accggaggcg gcgatggtgc caaacattac aagcgttgcg ccgcccagaa ccggttctgg
    15361 aatgtgttgt acaaaaccgc tcactgccgg gaacagaccc agcacgatca acatcagcgc
    15421 gacgacaaaa ccgacatagc ggctggcaac accggttaac tggatcaccc cgttgttctg
    15481 cccgaagcag gagttcggga aggtattaaa taccgccgag acaaacgagt tcaggccgtt
    15541 tgccagtacg ccgcctttca ggcgtttcat gtacagcgga ccggacactg gctgttcgga
    15601 aacgtcagag gtcgccgtga tatcgccaat ggtttccagc gaagtgatca taaagaccag
    15661 catcagcggc agcagcagac tccattcaat gccaagaccg taatagagcg gcgttggcac
    15721 cataatcagt tcttgtgtca tcggttcgtt gctttctggc aacatgccca taaaccacgc
    15781 cagcgcatat ccggccgcca tcgcaatcac cagtgaggcc acgcgtaagt aagggttacg
    15841 ttgacggtta agcaggataa ttaaggctaa gaccacgcct gccagcagca gatttttcgg
    15901 tgcgccgaag gtgttatcgc tcatggctgc gtaaccgccg ccaatggagg tcagcccaac
    15961 ctgaattagc gacaggccga taatcatcac cacaacgcca gaaaccagcg gcgtaataat
    16021 gcggcgcgcc agatgcagaa cgcgggagat caccatctcg gtacaacttg ccagcatcaa
    16081 cgtgccgaac aaagctgcca tcatggtagg aacatcagca ccaccggtct tcagcgctgt
    16141 gccgcccata atcagcggag caacaaagtt gaagctggtg ccctgaatag acaacaaccc
    16201 ggagccaacc ggaccccagg ccttaatttg aataatcgat gccacaccgg aggcaaacag
    16261 cgacatacta ataatgtgtt gcgtgtcttg tgccggtaaa cccagcgcct ggcagattaa
    16321 tagcgctggc gtgatcaccg caacgaacat cgccagcaaa tgctgacagg cggcaaacag
    16381 ggtttgagga agcggcggac gatcttcaag acggtaaatc agttcgctgt tttgagtctg
    16441 cgcaaccggt tgcgcatttt ctgactcgag ggtggaaaca gacatcgaaa acaatcccgt
    16501 ggtagcaaag cgggcatttt agctgaccga aagagaaaag caaacgtttg ccacatcctc
    16561 atctaaatat ttaaatgaga taaatgtact tttttatata acttttagtt gttttctgct
    16621 taaaatccat gccatttttc tagtttcagc taagaattcg aaccaggaaa gcatgacccg
    16681 atactcgtca tatttcgcct tactgcaaat gcgcgatgaa gtatgacgag tatgaaagag
    16741 tgatgcggac acaaaggagt aactatgttt catctcgata ctttagcaac gcttgttgcc
    16801 gcaacgctga cgttgctgct cgggcgcaag ttggtccatt ccgtctcctt tttgaagaaa
    16861 tacaccatac cggaacctgt tgcgggtggt ttattagtgg cgctggcgct gctgatactg
    16921 aaaaaaagca tgggctggga agtcaacttt gatatgtccc tgcgcgatcc gttgatgctg
    16981 gctttcttcg ccaccattgg cctgaacgcc aaccttgcca gtctgcgagc tggtgggcgt
    17041 gtggtggggg ttttcctgat tgtggttgtt ggcctgttgg tgatgcaaaa tgccattggc
    17101 attggtatgg ctagcctgtt agggcttgat ccgctgatgg ggctgctggc cggttctatt
    17161 acgctttccg gcggtcacgg tacaggtgct gcatggagta aattgttcat tgaacgttat
    17221 ggcttcacca acgcgacaga agtggcgatg gcctgtgcaa cgttcggttt ggtgctgggc
    17281 ggcttgattg gcggtccggt ggcgcgctat ctggtgaaac actccaccac accgaacggt
    17341 attccggatg accaggaagt tccgactgcg ttcgaaaagc cggatgtggg ccgcatgatc
    17401 acctcgctgg tgctgattga aactatcgcg ctgattgcta tctgcctgac agtggggaaa
    17461 attgttgcgc aacttttggc tggtactgct tttgaactac cgaccttcgt ctgtgtacta
    17521 tttgttggcg tgattctgag caacggtctg tcaatgatgg gcttttaccg cgtctttgag
    17581 cgagcggtat ccgtgctggg taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg
    17641 gggctgaaac tgtgggagct ggcttcgctg gcgttgccga tgctggcgat tctggtggta
    17701 cagaccatct tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac
    17761 tacgatgcgg cagtgctggc tgcgggtcac tgtggttttg gccttggtgc gacaccaacg
    17821 gcaatcgcca acatgcaggc gatcactgaa cgctttggcc cgtcgcacat ggcgttcctg
    17881 gtcgtgccga tggtcggtgc gttctttatc gatatcgtca atgcgctggt gattaagctg
    17941 tatttgatgt tgccgatttt tgccggttaa ccgatgaagc ggcggtcttc tcaccgccgc
    18001 tttttacgca ttcgagtaac gttctgtttc tggcatccag cgttctatca gggcttttgc
    18061 ctgttgtggg taacgttcgt gaatatgtcg cgccaggcgc tgaacttccg ggatcatcgc
    18121 ctgatcgcgc agtaaatccg ccactttaaa ttcagcattc cccgtctgac gtgtacctaa
    18181 caattcgcca gggccgcgaa tctccagatc tttctgtgca atcacaaaac catcgttgct
    18241 gtcgcgtagt acctgcaggc ggatttgcgc ggttttggag agcggcgttt tgtagagcag
    18301 cacgcagtga gaggccaccg cgccacgacc cacgcggcca cgtaactggt gtaactgcgc
    18361 cagacccaga cgctccgggt tttcgataat catcagactg gcattgggta catcaacacc
    18421 gacttcaatg acagtagtgg caaccagcag gtgtagctcg ccttgtttaa acgatgtcat
    18481 caccgcctgt ttctcggcgg gtttcatccg tccgtgtacc aggccaacat tcaactctgg
    18541 tagcgccagt ttcaactctt cccaggtcgc ttccgccgcc tgcgcttcca gtaattccga
    18601 ctcttcaatc aacgtacaaa cccagtacgc ctggcgccct tcagtcatac aggcgtggcg
    18661 cacgcggtca atgatatcga tacggcgggt atcaggaata gcgaccgtag tcactggcgt
    18721 gcggcctggt ggcagctcat ctatcaccga ggtatcgaga tcggcatacg cagtcatcgc
    18781 cagcgtgcgg gggatgggcg tggcggtcat gatcaactga tgcggatgga agccctgctg
    18841 ctggcctttc tcccataacg ccagacgctg atgtacgcca aaacgatgct gttcgtcgat
    18901 aatcaccagc gccaggccgt taaactgcac ctgttcctgg aaaatggcgt gcgtaccaac
    18961 aatcatctgc acctgaccgc tggcgatggc ttcttgctga gccagtcgtg ctttaccttt
    19021 ctgttttccg gcgagccagc ctacttcgat accgagcggt gcaaaccagt tacggaagtt
    19081 attggcgtgc tgctcggcga gtaattcggt tggtgccatc agtgccacct gtttgccgtg
    19141 ggcaaccgca cgcaacgcgg cgagggcggc gaccagcgtt ttaccggaac ctacatcgcc
    19201 ctgcaccaga cgcatcatcg gcacatccag cgccatatcg cgctcgatct ccgccactac
    19261 gcgtgcctgt gcgcccgttg gcttgaacgg taaggcggcg aggagtttat ttttcagcgc
    19321 gtcattggcg ctcagcggct gagcatgaaa acgttgtgca ccggcacgta acgccaacat
    19381 gctgaggttg tgcgccagca actcttccag aatcagccgg cgttgtgccg gatgctgccc
    19441 ggtttcgaga tcgctaagct gtagcgtcgg cggcgggcgg tgcaaagtgc gcaacgcttc
    19501 cggtagcgtc atcattcctt gtgacagttc cggcggcagg agttcttcaa tggcgcatgt
    19561 gtcgagcaga tccagcgcct ggtcggttaa tttacgcagc gtagcctgct ttacgccttc
    19621 cgtagttgga taaaccggtg tgagcgtttc ctgtaactct ggcgtgctga gatcgccctg
    19681 tacgcggtat tccgggtgga tcatctccgc accatatttg ccgcgctttg cttcgccata
    19741 ggccagcacg cggcggcctg tcgccaggct atttttcatt gccgcattga agttgaaaaa
    19801 gcgcatagtg agaatgccgg aaccgtcgct gatctggcag gtcatcatcc gccgaccgcc
    19861 gaaggagata ttgcagttca gcacttcgcc ttccaccgtg gcataaacac ccggcagtag
    19921 ttcaccgatg gggtagagat gtgtgcgatc ttcgtaacgc agaggaaggt gtaagagtaa
    19981 atcctgtacg gtatgcaggt tgattttcgc cagcttgtta ctaagcgctg cgccaacgcc
    20041 cgttagggaa ctgagcggga cagcatctaa caggcgacct ttcatcgcac ttaccctgca
    20101 gcctgcatgg tggaccacca gtcagcatcg gcttcaatct cgccttgctg attaacgtgg
    20161 gggtagggca gacctttgcg ttttgcgact ttcgccagca ccggatagcc gccttcaaac
    20221 agcaggcgtt gctgctcggc ttccggcaac atgctgtttt cacgcaggta catgcccgca
    20281 ttttgccgct ggcgctgggc ttcgtaaaga atgagggctg aggcaacgga aacattcagc
    20341 gactgcacca tgccgatcat cggaatgatg atatcctgat ccgccagggc caacgcttcc
    20401 tgcgtgatgc ccgttttctc ctgtcccatc aaaatacagg tcgggcgagt gtaatcaatt
    20461 tcgcggaaat caacagcgtt atcagaaaga tgggttgcca gaatctgcat gccctggcct
    20521 ttcagatgag cgacggcatc gccaatggtg cggtgcgttt tcacctgtac ccagctgtta
    20581 ctacccgcag ctgccgaagc catggtgcgc atccggctgc caggccagac agcatgaact
    20641 tcatgtacgc caacggcatc tgcggtacga ataatcgcag aaacgttatg gggtttgtgg
    20701 acctgctcca tgcagacggt cagatcaggc tgccgcctgg cgagcatttc gcagatgcgt
    20761 gcataacgtg ttgggttcat aaaacattaa tttcggtttc gggtgacttt aatcacgtct
    20821 ggcatcacgc ggattttgcg catgatattc gccagatgca cacggtcacg ggcggtcaga
    20881 cgaataaagg cgctatagac gcgaccatct ttctcttccg tattcaaact ttgaatattc
    20941 gaggtcgtgg tgttaattgc cgccgtcagg tttgccagcg cgccctgatg attgaacatc
    21001 tccaccttga tttcggtgat gaattcctgc gccgtctctt tatcccattc aacagccata
    21061 aacttctctg gctctttctg gtagccacgg atgttacggc aggattcatg gtggatcacc
    21121 agacctttac cggggctgac gtgggcgata atcgggtcgc caggaattgg gcggcagcat
    21181 ttcgcaaagg tgatcagcac gccatcggca cctttaatgg gcagatgtcc gtgactttgg
    21241 gttgccggtg gaatggaggc gtccccatgt tgcagatttt tcgcgaccac cacgctcatt
    21301 gcgttaccaa ggccgatttc agccagcaga tcgtcaagcg tagccagctt catgcggtcc
    21361 agctcgcgct gaatattttc ctgcgggatt tcattgagct tgcggctgcc acccaacgca
    21421 tggttgagca gacgacggcc caggcttaca gaatcatcac gcttgaggtt tttcagcaat
    21481 tgacgaattt tggcgcgcgc tttcgagcta acgacaaagt tcagccacgc ggcattcggg
    21541 cgagcgcccg gtgcggtaat gatttcaacg gtttgaccgc tggtcagcgg ctgcgacagc
    21601 gggtaaggct gacggtcgac acgcgcgccc acgcaggcat gaccgatatc ggtatgcacc
    21661 gcataagcga agtcgacggg cgttgcaccg gcaggcagct ctacaatgcg cccttccggt
    21721 gtgaaaacgt aaatctcatc ggggaagaga tcggatttaa cgctctcgat aaattcaaac
    21781 gaactaccgg cgctctgttg cagctccagc aggctttgca tccagcgctg ggcgcggatt
    21841 tgtgcggtag tactggtttc gccgtgctct ttataagccc agtgcgcggc aacacccatc
    21901 tccgccatct gatccatatc ttcggtacgg atctggactt caaccggcac gccgtgcggg
    21961 ccaatcatcg aggtgtgcaa agactgatag ccgttcgctt ttggaatggc gatgtaatct
    22021 tttacgcgac ccggacgcgg cttgtacagg ctatgcattt gcccgagcac gcgataacag
    22081 gtgtcggaat cattgacgat cacgcggaag gcgtagatgt ccatgattga gtgaaaacgc
    22141 tgctctttga gcaccatttt gcagtaaatc gaataaagat gcttctcgcg accactgacg
    22201 cggcacggta ttcccgcttc ctgcaaacgc ccttcgattt cagaaagaat cttctggatc
    22261 atctctttac ggttgccgcg cgcggctttc accacttctt tgattacgcg ataacggttg
    22321 ggatacagcg cctcaaaacc cagctcttcg agttcggttt taatgtggtg gatacctaaa
    22381 cggtgcgcca gcgggctgta aatttcgagt gtttcacggg cgatgcggcg acgtttgtcc
    22441 gggcgaagtg agcccagcgt gcgcatgttg tgggtacggt cggcaagttt gatgagaatg
    22501 acgcggatat cctgcaccat cgccataatc atcttgcgaa agttttcggc ctgcgcctct
    22561 ttcttatcgc ggaacttgag cttatcaagt ttcgacaccc cctctaccag ctcggcgacg
    22621 cttttaccaa aaagctgttc catgtcctgg taggtggcgg gagtatcttc aatcacgtca
    22681 tgcagcagcg ccgccatcag cgtttcatag tcgagtttca tctcggccag aatgcaggca
    22741 accgccaccg ggtgcgtgat atagggttca ccgcttgaac gtgtttgccc ctcgtgagca
    22801 tcacgtgcaa cgagatacgc ctgccgcaga cgcttgattt ggtcttccgg caggtaggtt
    22861 tgaatcagtt gattcaggct ttcaaacaga tacaagggcg acccgctttg tgattaacga
    22921 cgaccttcag caatagcggt aacggcttgt aattcagcgg cttcctgctc ttgctgttcc
    22981 tggcgttcac gaacgtcgag gatctggttg ttgatcagac cttcttcgat ttcgcgcagc
    23041 gcgattacag tggttttatc gttttcttcc ggtaccagcg gatcctttcc gcctacctgc
    23101 atctgacgag cgcgacgcgc ggcgaccagt accaggtcaa aacggttacc aattttctct
    23161 acagcgtcct gaacagttac gcgtgccata cttaaaaagc tccacaggtg aagaaatgac
    23221 tgggcatgat actgaaatca ggttcagtct gccaacaatt tgctgattaa agcgtcatga
    23281 cgctgctttt ggcggctcat gcgcagacgt tcggcgcgaa taatggtctt caaatcggtc
    23341 aacgcggtat cgaagtcatc attcacaatc aggtaatcat attcggcgta atggctcatt
    23401 tctgcaacag cttgcgccat acgctttgca atgacctctt cgctgtcctg accgcgaccg
    23461 cgtagacggc ggtccagttc aattttggac ggcggtaaaa taaagatact ccgcgcgtgc
    23521 ggcatcttct ggcgaatttg ctgcgcgccc tgccagtcga tatcgagaaa aacatcgaca
    23581 ccggtcgcca gtacttgctc aatggcctca cgcgaagtgc catagtaatt accaaaaact
    23641 tctgcgtgtt cgaggaacgc atctctgcta atcatttctt taaattcatc atgattaaca
    23701 aagaaataat gttcaccgtg gacttcacca ggacgcggtt ggcgtgtggt gtgtgaaaca
    23761 gaaacttggg tgtcatacaa cggttgggtt tttaataaag cctgaatcag gctggattta
    23821 cccgcgccac tgggggcaga aacaatataa agcgtgcctt gagccatgag tatctttagt
    23881 atgtgattag cgaaataaag cctacatacg agcttattat acacggcgcg gccatgtgac
    23941 gtagcccttg ctcacacttt ttgcgcaaag ttgttgcgtt ttgcgtagct gcttccgcat
    24001 ttgcctgtga ttcactgcaa taaagacgat tctcaggaag cgtcctcgca gactccgttt
    24061 ttgccactcg gctttgcttt catcacgttg tatatctgcc tgactatcta cagcggaggt
    24121 gatgatgaaa gtatggatgg cgatattaat aagtatcttg tgctggcaat catcggcgtg
    24181 ggcagtctgt ccggcctggt cgccagccag agcacaggaa gaaatttccc gcctgcaaca
    24241 gcaaataaaa cagtgggacg atgactactg gaaagaaggg aaaagtgagg tggaagacgg
    24301 tatttacgat cagttaagtg cccgtcttac gcagtggcaa cgctgttttg ggaacgagac
    24361 ccgcgatgtg atgatgccgc ctttaaacgg tgcggttatg catcctgttg cccatacggg
    24421 tgtacgcaaa atggcggata aaaacgcatt aagtctgtgg atgcgcgaac gtagcgatct
    24481 ttgggttcag ccaaaagttg atggcgttgc ggtaaccctg gtttatcggg acgggaaact
    24541 gaacaaagca atcagtcgcg gtaatggcct gaaaggcgag gactggacgc agaaagttcg
    24601 cttaatttcc gctgtgccgc aaaccgttag cgggccttta gccaacagca cgcttcaggg
    24661 ggaaatattt ctcaagcgta aggggcatat ccaacaacaa atggggggaa taaatgctcg
    24721 cgcaaaagtt gctggcctga tgatgcgcca ggggaatagc gatacgctaa actcactggc
    24781 tgtttttgtc tgggcatggc cggatggacc gcagttaatg accgatcgtt taaaggatct
    24841 ggctaccgca ggttttactt taacgcagac gtatacccgc gcggttaaaa atgctgatga
    24901 ggttgcgcac gtacgcaatg cggggtggaa agcgaaatta cccttcgtca ccgatggcgt
    24961 ggttgtgcga gcggctaagg aacccgaatc ccgtcattgg ctaccgggcc aggcggagtg
    25021 gctggtggcc tggaaatatc aacctgtagc tcaggttgcc gaagtgaagg cgattcagtt
    25081 tgcggtgggt aagagcggta aaatatcggt ggttgcgtca ctcgcacctg tcatgctgga
    25141 tgataaaaaa gtccagcggg tgaatattgg ttccgtcagg cgctggcagg agtgggatat
    25201 tgcgcctggt gatcagatcc tcgtcagcct tgccggtcag ggtattcctc gcattgatga
    25261 tgtggtctgg cgcggtgcag aacgtacaaa accgacacca ccagaaaacc gctttaactc
    25321 gttgacctgc tactttgctt ctgatgtttg tcaggaacag ttcatttcac gcttagtctg
    25381 gctggggtca aaacaggttc tggggcttga tggcattggt gaggccggct ggcgcaccct
    25441 gcatcagact catcgctttg agcatatctt ttcctggctt ttattaacgc cagagcaatt
    25501 acagaacacg ccggggatcg cgaaaagtaa aagtgcgcag ttatggcatc agtttaatct
    25561 ggctcgtcag cagcctttta ctcgctgggt gatggcaatg ggaataccgc taacccgggc
    25621 ggcgcttaat gccagtgatg aacggtcctg gtcgcaactt ttatttagca cggaacagtt
    25681 ctggcagcag ctgccgggga ctggatcggg gcgcgccaga caggttattg aatggaagga
    25741 aaatgcgcaa atcaagaagc tgggcagttg gctggctgcc cagcagatca caggttttga
    25801 accttagtga ccttcgtggc tgtagtaaaa gaccggtaat cccagcttaa gacgtagcgc
    25861 cagtaaacgg gcaaaaaagc cgaataccag ggtggagata atcaccacat catggttaga
    25921 aacatagtgt tgcagcgcga tgtacagcac ggcggaggca aatgagacac cggcatacaa
    25981 ctctttctgg aataccagtg gaatgcgttt acagaacata tcgcgcaaaa cgccgccaaa
    26041 cacgccggtg gttaccgccg cgacaacggc aataatcggg ccgtgcccca tatccagtgc
    26101 aacctgtgcg ccgatgatag aaaagaccac cagtccgagc gcatcaagca ccagaaacac
    26161 tttgcgcaga taaggcatta caggagcaac tatagtggtg agtactgcgg cggtagcgac
    26221 gataatcaca tattccgggt gcttgaccca accgagcgga taatggccca gcagaatatc
    26281 gcgcacggac cctccgccaa ttgcggtagc agtcgcaata ataattacgc caaatgtatc
    26341 catgcgccgt cgtccggccg ccagcgcccc cgtcatggct tcggcagtaa tgccaaccaa
    26401 atacaaaatg tgtaacagca tggttctctc cctaaaaatc tgtctggaag ggtagcgatt
    26461 tgtgcgtatt gtcacgattg agatttttta aggtgagagg aaattgatga cttgtagtta
    26521 tgtgaaatcg ctattttctg tagcgtagat gcattcttct gacttcttgt ctgagaaaat
    26581 gcataagaaa aaataggtta aaattgaggg gtggaaaaga aaggtgcaat gtttagccat
    26641 tgtgttcacc gcacctggac tttggcttac gcatcgggta tctcaatcaa caaaaagagt
    26701 ttttagtttc tgctatttct tctctgtaat ttttacactg ttttccaact gcttgatgct
    26761 gacctgcggg gtcggcaact cctcaggcat ggttccgcca agttcctgaa tggtttgcct
    26821 caccttgcga cccacgtcaa agtgtgtggt attagcttgc tgttttgaat tcacgccatc
    26881 ccgcttgagt ttttcttctg tttgggtagc tcgaaagaga ttagccgcca gttctgttga
    26941 acccatatga tccaggatct tctgactctt tttcagcccc ttccgctgat gaatagcttt
    27001 ctgatctaat ccaccataca gtccccggta accatgattc tgaaatatgg cgaaatcgat
    27061 agctgtcgct accccagctt gctgcgctgc ctcaactaat tgtttgttat gttctttcaa
    27121 ctcatttctc agaaaaagac gcttttcgtc ttcacgaagt tgcctgaaag cttcatcgtc
    27181 agccagctcc tgccgtcggg tctggatagc aaaataagtt tgccctaccg caataaccgg
    27241 tttcgcaggg tcgccgtttt gtactaccag gtaacaggca tagcgggaga gatgaacatc
    27301 ttcaagttct cgttgagccc ctgaacctaa tacaaccatt ttggtggttt caacgaaatg
    27361 gtcagatgca gcttgattgc tggcttcgca cgcttgagtc gcgcgggcca gtaccttttg
    27421 aaaattgcgc cagtcccgat aatccagtaa cggtgccaat tctctcgctg accaaaattc
    27481 ttgcccttca gtgccataat gccgtatctc ttcaaaaggt tgatgatgtt cgttcattgc
    27541 ccactccatg tgatatcgat tgcaaagaaa gtaaatctgt aactgtattt atatacagtt
    27601 gtgagtatct cacgttctca gtgtgctggg caaaaaaaca tcgcaaaatc tggagcaggc
    27661 acgcaaaaca gaattgaagt tgcggggaag aaaaatgccg ctctgtttga gtgggcggca
    27721 tttgtgaagg ctgaaaagta agaattggcg ttacgagtta ttcaatgttc tgaatctgct
    27781 cgcgcatctg ctcaatcaac actttcagct caatcgcgga gtttgtcact tcggcattga
    27841 tagacttcga agcaagagtg ttcgactcgc ggttgaactc ctgcatcata aagtccagac
    27901 gacggccaac cgcttctttt ttcttcagaa tgttgtaggt ctctttgaca tgcgcttcga
    27961 ggcggtccag ttcttcggca acgtcaattc gttgtgccag cagaaccaac tcctgctcca
    28021 gacggttgtt ttccagttgc acctgagcat cttccagctt cgctaccaga cgctcacgct
    28081 gccattgcag gatttccggc atatgggcgc ggactttgac cacttcggcg gtgacgcctt
    28141 ccagacgctg ctcaatcaac actttcagcg cctgaccttc ggtttcgcgc gcgatgataa
    28201 agtcatccag ggtgccatcc aacgccgcga gaatttcagc tgcaatcgcg tcgagatcct
    28261 gctcctgggc tgccatcacg cccggccagc gtagaatatc aaccgggttg atttctcctt
    28321 cgtcgctctg catttttacc cagttcgcgg cagttaccag ctgtttagcc agtttttcgt
    28381 tgaggatcag ctcaccttgc gcgctaacat ctggctcata gcgcagagta cattctactt
    28441 taccgcgcgt caggcgagaa cgaatacgct cgcgaacgac aggttcaagg ctacggaact
    28501 gctccggcag acgaaagtaa gtttccagat aacgctggtt taccgagcgc atttcccagg
    28561 ttgcgctccc ccattcaccc ttgatttcac gccgggcgta ggcggtcata ctgcggatca
    28621 tagacgttcc tgtttataaa aggagaggtg gaaggattat agccatcgat gccttgtcag
    28681 gataggaata accgccggaa gtccgtataa tgcgcagcca catttgtttc aagccggaga
    28741 tttcaatatg cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta ccctgactcg
    28801 taactataca aaacatgcag aaggctcggt gctggtcgaa tttggcgata ccaaagtatt
    28861 gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg gccagggctg
    28921 gatcaccgca gagtacggca tgctgccacg ttctacccac acccgtaacg cgcgtgaagc
    28981 ggcgaaaggt aagcagggtg gacgcaccat ggaaatccag cgtctgatcg cccgtgctct
    29041 tcgtgcggca gtagatttga aagcgctggg tgagttcacc attacgctgg actgcgacgt
    29101 gcttcaggct gatggtggca cgcgtaccgc gtcgattacg ggtgcctgcg tggcgctggc
    29161 agatgcgcta cagaagctgg tggaaaacgg caagctgaaa accaacccga tgaaagggat
    29221 ggtagccgca gtttctgtcg gaattgtgaa cggcgaagcg gtttgcgatc tggaatacgt
    29281 tgaagactca gccgcagaga ccgacatgaa cgtagtgatg accgaagacg ggcgcatcat
    29341 tgaagtgcag gggacggcag aaggcgaacc gttcacccac gaagaattac tcaccttgtt
    29401 ggctctggcc cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg cgctggcaaa
    29461 ctgattttta aggcgactga tgagtcgcct tttttttgtc tgtagaaaag taagatgagg
    29521 agcgaaggca tgaaaccata tcagcgccag tttattgaat ttgcgcttag caagcaggtg
    29581 ttaaagtttg gcgagtttac gttgaagtcc gggcgcaaaa gcccctattt cttcaacgcc
    29641 gggctgttta ataccgggcg cgatctggca ctgttaggcc gtttttacgc tgaagcattg
    29701 gtggattccg gcattgagtt cgatctgctg tttggccctg cttacaaagg gatcccgatt
    29761 gccaccacaa ctgccgtggc gctggcggag catcatgacc tggacctgcc gtactgcttt
    29821 aaccgcaaag aagcaaaaga ccatggtgaa ggtggcaatc tggtcggtag cgcgttacaa
    29881 ggacgcgtaa tgctggtgga tgatgtgatc accgccggaa cggcgatccg cgagtcgatg
    29941 gaaattattc aggccaatgg cgcgacgctt gctggcgtat tgatttcgct cgatcgtcag
    30001 gaacgggggc gcggcgagat ttcggcaatt caggaagttg agcgtgatta caactgcaaa
    30061 gtgatctcta taatcaccct gaaagacctg attgcttacc tggaagagaa gccggaaatg
    30121 gcggaacatc tggcggcggt taaggcctat cgcgaagagt ttggtgtttg ataaaaaacg
    30181 ccggatggta aatcatccgg cgtcatctta ctgcaactgc gcagcaatca gtggccagcg
    30241 ggcgtcaaaa tcatccgtcg ggcggtattt aaattcgctg cggacaaagc gtgacagcat
    30301 accttcacaa aatgccagca actggcttgc cagcagcgtt tcatcggtgg tgtaaccttc
    30361 accctcacgc attctctttt cacgcaatac ctgacgcaac tgcgcctcaa tacgctcgaa
    30421 cagctggttg atgcgccctt gcaggcgatc ctgttcaaac attagtgcat gaccagtgag
    30481 gatgcgggtc aggccaggat tacgctcacc aaaaccgaga agcagcaaca caatcagacg
    30541 caggcgcgct gtggtgtcct tctcatcttt cagaatcaga ttgatgcgag taatcaggct
    30601 atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga agtggcgata
    30661 cagtgccgct tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga tacgttggct
    30721 tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc gacggttcct
    30781 tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc acttgccagg
    30841 cggcacccac gctatgaccg caaacgaaat gtttgcggct atgttatgac gttattcgga
    30901 tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg tcggtggcgt
    30961 cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc atctgggcga
    31021 tgcgttcgcc aggttgaatg gtgaagctgt cctgaccacg gttccacacg gaaatcatca
    31081 actggccctg atagtcagaa tcgatcaatc ctaccaggtt accaagcacg ataccgtgct
    31141 tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg gcaatatgaa
    31201 tcgccagccc ggtcggaacc agcgtagtgt cacccggagc cagttctacg gcgtcgtcga
    31261 gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg agcggaaatt
    31321 ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata acgggtcacg
    31381 atctcgtcga gtaataattg gccaaggagc gctttgcgct caagcggtaa gactttatct
    31441 ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg agttggctgg
    31501 gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg ccgggcgtat
    31561 tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg gtcttttagt
    31621 gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgttaa ttcatcaccc
    31681 tgcgtggcct gctttttgat tttctctggg gccacggtag ctgcgcgata atccgccacg
    31741 gcggcgcaac cgataaaaat attttgctgc tgtacagatg cattcacggc ggcttccatt
    31801 tccagcgcgg tcatcacatc aacacgttta acaaacggtg gcgtcggtag cgaaaccgga
    31861 cctgatacca gcgtgacgtt cgcgccacga cgggcagcgg cggcggcgat agcaaaaccc
    31921 atcttgccgg agctgtgatt agagatataa cgcaccggat cgagcggttc acgcgtcggg
    31981 ccggcggtaa tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa atgcgccacc
    32041 gccatatcca caatggttaa cggatcgagc attcgcccag ggccgatatc accacaagcc
    32101 tgactgccac tgtctggccc ccagatgagc aaaccacggg aagcaagtac ctctaaatta
    32161 tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac ggctacaggc
    32221 gcaggtgtgg ccagacaaat cgtcgatacc agatcattcg ccattccggc agcaacacgg
    32281 gcaatcaaat ctgccgtggc aggggcgaga atcactaaat cagcccattt acccagctca
    32341 atatggccca tagcggcttc ggctgccggg tccagcagac tgtcggaaac gggataacca
    32401 gaaaccgcct gcaagctaag tggggtgata aaggcttttg ccgcttcggt catggctacg
    32461 cggacatcgg ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt ataggcagca
    32521 ataccgccgc taacgccgag aacgattttt ttaccggcca ggctcatgat gatttttcct
    32581 gttgggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg tgccttcacc
    32641 tgagattcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat ttaacggaac
    32701 ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat gcacagctgt
    32761 tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgc cttaacggat gtcgagctgc
    32821 tggcgctatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg gcaaaagaga
    32881 tgctggagaa tttcggctct ctttatggct tgttaacctc tgaatatgag caatttagtg
    32941 gcgttcatgg aattggcgtg gcgaaatttg cccagttaaa ggggattgct gaactggcgc
    33001 ggcgttacta caacgtacgg atgcgtgaag aaagcccttt actcagcccg gagatgacgc
    33061 gggaattttt acaaagccag ctcacgggcg aggagcggga gatctttatg gtgatctttc
    33121 tcgactccca gcacagggtt ataacgcata gccgtctttt ttccggcacg ctaaaccatg
    33181 ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaacgcc tcggcgctga
    33241 tccttgcgca taatcaccct tcgggttgtg ctgaacccag taaagcggat aaactcatta
    33301 ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac catatcgtga
    33361 ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc gctatgcgcg
    33421 atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag cgtatactac
    33481 gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt tcacatagaa
    33541 ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga agctcgagct
    33601 aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc aagcgtccgg
    33661 tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc ctgccgaatc
    33721 tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg cgcgtatctg
    33781 ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct gaactgcgtg
    33841 cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag gtattcgtga
    33901 gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta cgaagaacaa
    33961 acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc gccagcacgt
    34021 gatctacaaa gaagcgaaaa tcaaataatt tccgctttga tgtaacaaaa aacctcgctc
    34081 cggcggggtt ttttgttatc tgcttgcccc catattgact gcatctgttc attcctggag
    34141 atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg cggcatagaa ccgcatctcg
    34201 ttggtgcaac cattcttcat gcggtggtgc gcaacggacg cttgcgctgg ccggtttcag
    34261 aagagatcta ccgtttaagc gaccaaccag tgcttagcgt gcagcgccgg gctaaatatc
    34321 tgctgctgga gctgcctgag ggctggatta tcattcattt ggggatgtct ggcagcctgc
    34381 gcatccttcc agaagaactt ccccctgaaa agcatgacca tgtggatttg gtgatgagca
    34441 acggcaaagt gctgcgctac accgatccgc gccgctttgg tgcctggcta tggaccaaag
    34501 agctggaagg gcacaacgtg ctggcccatc ttggaccgga gccgcttagt gacgatttca
    34561 acggtgagta cctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa ccgtggctga
    34621 tggataacaa gctggtggta ggcgtaggga atatctatgc cagcgaatcg ctgtttgcgg
    34681 cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcggagtgt gaattgttag
    34741 ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggcggtaca acgctgaaag
    34801 attttctgca aagtgatggt aaaccgggct atttcgctca ggagttgcag gtttacgggc
    34861 gaaaaggtga gccgtgtcgg gtgtgcggta cgccgattgt ggcgactaaa catgcgcagc
    34921 gggcaacgtt ttattgtcgg cagtgccaga agtaattgat gcgcgccgga tggcatacca
    34981 tccggcataa acgctacgct aacttcgcca tcagcgcctg atggacattc tccggcagga
    35041 aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa gagataaacg
    35101 accactcttt cgacggcatc agaaacacac tttccagctc cggcattaag tggcgattca
    35161 tatgcgccag ctgcatttca tattcaaaat ccgccaccgc acgcaggcca cgaatcagca
    35221 ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg accacttcca
    35281 cgttccccag atgcgcggtt gcctgctgtg ccagctccac acgctcttca agggtaaaca
    35341 tcggtttttt actgggacta gcggcaatcg ccagaataac gtgatcgaac atttgcgtgg
    35401 cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtaccc ggataaatcg
    35461 cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc cagcagttga
    35521 agcagacgct gtagcgcgcc ctggttttga tacagtactt caacggcatg acggccatag
    35581 aaactacggt aatcggcgtc ggtgagtaaa gaggaaacct cttttgcaag cgacgtggca
    35641 tcggtaacgg taatcagccc gcttgcctgc tccagccgcg cgcaaatgtc tttaaagtta
    35701 aaagtatgcg gccccatcaa taccggaata gcgtgtgcgg cagcttccag cggattatgc
    35761 ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc atacagtaac
    35821 atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg gacttcccct
    35881 gaagagcgtg tgatatagct tagtccagcc tggcggacaa ggttaatcgc atccgggaag
    35941 cgttccggat gacggggtac caggatgagc aataaattcg ggaattgctg taacaatgcc
    36001 tgatgtgcgg cgatcaccac actctcttcg ccttcgtgag tgctggtggc aatccatacc
    36061 gggcggtgtg gtgcccactg gcggcgcagc gtcactgctt tagcagccag ttgcggcgtt
    36121 acagaaatat cgaatttcag gctaccggta acggtcacct gattattttt tgcgcccagt
    36181 gatacaaaac gtgcaccatc ttcttcattt tgcgcagcaa tcagcgtaat acgacgcagc
    36241 aagcgacgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc agagagtcgc
    36301 gcgttagcga tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag gttaggccat
    36361 agttcggttt ccataatcaa caccagttta gggtcgactt tattcaggaa acgattaagt
    36421 gcatcgggca ggtcatatgg cagataaacg tgctgaacat ccttcccgaa agccgattgt
    36481 acgcgctccg aaccggttgg cgtcatggtt gtaacggtaa tcggtaaatc aggataacga
    36541 tggcgcagcg cgcgcaccaa cgggatcgcc gccagagttt caccgacgga gacggagtgc
    36601 agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc accccagcgt
    36661 tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat cagcggctga
    36721 ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg acttatggat
    36781 gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttcccg tttttgactt
    36841 aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt atttttatgc
    36901 gattcattga tttctgcagt acgtgcagga aggaatcgca gagcaacagt aaaagaatat
    36961 ggttgctaat gattttattt gattgatttc tttattgaaa atcaacaatg tgcttaaaaa
    37021 tcattcgagt cggattgccg cgaaagaagt gatacactag cgcgtcaata tcagtgcggt
    37081 atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg cttcacctta
    37141 atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag aattttgctc
    37201 attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag ttcgctgaaa
    37261 aaaaattacc ctgacgcaaa aatcgatgtg ctgctttatc aggacaccat cccgatcctg
    37321 tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc aaaagcctca
    37381 gaaaaaatta ccaacttttt tcatctcatc aaggtattac gtgccaataa gtatgacctt
    37441 atcgttaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt aaatgtccgt
    37501 gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa tagtttcact
    37561 catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt gctgacgcca
    37621 ttgggacttg aatcgttggt gaagcagaca accatgagtt acccgcctgc aagctggaaa
    37681 cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt tatccaacct
    37741 acggcgcggc aaatcttcaa atgctgggac aacgccaagt tttccgctgt gattgatgcc
    37801 ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga cgatctggcc
    37861 tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct ggccggaaag
    37921 gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat tggcgttgat
    37981 tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct gtttggtgcg
    38041 acagaccata ttttctggcg tccctggtca aataacatga ttcaattctg ggcgggagat
    38101 taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct ttcggttatt
    38161 ccggcggcag atgtcattgc agctgtcgat aaattacttc cctcctccac gacaggtacg
    38221 tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt ttgcagcgcg
    38281 attttatgcg tattgctcag acagttgccg cccgaggtca tcatgttcgg gtttataccc
    38341 agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg gttaaatcgc
    38401 ataccaatca cgggcgcaat gcggaatatt ttgcctgggt gcaaaaacat ttacgtgaac
    38461 accccgtcga tagagtcgtt ggatttaata aaatgccggg gctggacgtt tattatgccg
    38521 ctgatgtttg ttatgccgag aaagtagcgc aggagaaagg ctttttctat cgcctgacgt
    38581 cacgttatcg ccattatgcc gcctttgagc gggcaacctt cgaacagggc aagccgacac
    38641 agctgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat cagactgaag
    38701 cggagcgttt tcatattctg cctccgggga tttatcctga tcgtaaatat agccagcagc
    38761 cagccaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa caatatttat
    38821 tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga tcgttccatt gaagcacttg
    38881 cttcgttacc ggattcgctg cgccacaaca cattactata tgttgttggg caggataaac
    38941 cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt cacttcttct
    39001 cggggcgcaa cgatgtctcg gaattaatgg cggtggcgga tttattactt catcctgcct
    39061 accaggaagc ggcgggaatt gtgctgctgg aagcgataac tgcaggatta ccggtactaa
    39121 caacagccgt ttgtggctat gcgcattata ttgtcgacgc caattgcggc gaggctattg
    39181 ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta acgcaatctt
    39241 cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa gatttataca
    39301 gtctgccaga gaaagcggcg gacatcataa cgggtggtct ggatggttga acttaaagag
    39361 ccgtttgcca cgttatggcg cggtaaagat ccttttgggg aagttaaaac cttgcagggt
    39421 gaggtatttc gtgaactgga aactcgccgc actctgcgct ttgaaatggc gggcaaaagc
    39481 tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa tttactctca
    39541 ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg actgcgggat
    39601 gtcggcgttg atactatgta tggggtggca ttcggcgaaa aaggtattaa tccgctgacc
    39661 agaacctcgt ttattattac cgaagatctg acaccaacca tcagtctgga agattactgt
    39721 gctgactggg cgatcaaccc accagatgtt cgcgtaaagc gtatgcttat taagcgtgtc
    39781 gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg ttatatctgt
    39841 catttcctgc tgcacttgcc tttttccagt aaggaagagg agttaaaaat ttcagtaatt
    39901 gacctgcacc gggcgcagat acgcacgtgc gttccacgtc gttggcgcga taaagatctt
    39961 attggacttt atttttcttc gatgaatatt gggctgacgc agagagatat ctggcggttt
    40021 atgaaggttt attttgtcgc cccgcttaaa gacattctca agcaggaaca agggctgctg
    40081 tcgcaagcag aagaaaaagc cacaaaaatc agggaaagaa cgattcgaaa atcgttgtaa
    40141 tttccggaga gaaatatgag tgcccactat tttaatccac aagagatgat caataagaca
    40201 atcatcttcg atgaaaggcc agaggcgtca gtggcatcat cattccatgt tgcttatggc
    40261 attgatcaga actttctttt tggttgtggt gtttcaatca cgtcagtttt gttacataac
    40321 aacgacgtga gttttgtctt ccacgttttt attgatgata tccctgacgc cgatatccag
    40381 cgtttatccc aattggcgaa aagctatcat acctgtatcc agatccatct ggtaaattgt
    40441 gaacggctta aggtattacc gacgaccaaa aattggtcta ttgccatgta tttccgtttt
    40501 gtaattgcag attactttat tgatcaacaa gataagatcc tgtacctgga tgctgatatc
    40561 gcctgtcagg gaaccttaaa accgctgata acaatggatc ttgccaataa cgttgctgct
    40621 gttgttactg aacgcgatgc taactggtgg tcgttacggg ctcaaagttt gcagtgtaat
    40681 gaacttgaaa agggttactt taattcaggt gtcctgttaa ttaatacact agcgtgggcg
    40741 caggagtccg tttctgctaa agcgatgtcg atgcttgctg ataaagc
//
LOCUS       DADSZD010000021        40697 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.021, whole genome shotgun sequence.
ACCESSION   DADSZD010000021 DADSZD010000000
VERSION     DADSZD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.021"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            130..894
                     /locus_tag="J1Z28_001007"
     CDS             130..894
                     /locus_tag="J1Z28_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7715862.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(933..1859)
                     /locus_tag="J1Z28_001008"
     CDS             complement(933..1859)
                     /locus_tag="J1Z28_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7715863.1"
                     /translation="MNHSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     VILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            1949..2947
                     /locus_tag="J1Z28_001009"
     CDS             1949..2947
                     /locus_tag="J1Z28_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBA7715864.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2944..3162)
                     /locus_tag="J1Z28_001010"
     CDS             complement(2944..3162)
                     /locus_tag="J1Z28_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBA7715865.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKIEGLTQLVQPLKRPL"
     gene            complement(3164..5179)
                     /gene="ligA"
                     /locus_tag="J1Z28_001011"
     CDS             complement(3164..5179)
                     /gene="ligA"
                     /locus_tag="J1Z28_001011"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBA7715866.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLMQFKAWGLPVSDRVTLCESAEEVLAFYHEVEKD
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(5250..6248)
                     /gene="zipA"
                     /locus_tag="J1Z28_001012"
     CDS             complement(5250..6248)
                     /gene="zipA"
                     /locus_tag="J1Z28_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBA7715867.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEVVIIMNVAAHHGSELNGELLLN
                     SIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6478..7239
                     /gene="cysZ"
                     /locus_tag="J1Z28_001013"
     CDS             6478..7239
                     /gene="cysZ"
                     /locus_tag="J1Z28_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBA7715868.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVLQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7424..8395
                     /gene="cysK"
                     /locus_tag="J1Z28_001014"
     CDS             7424..8395
                     /gene="cysK"
                     /locus_tag="J1Z28_001014"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBA7715869.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8779..9036
                     /gene="ptsH"
                     /locus_tag="J1Z28_001015"
     CDS             8779..9036
                     /gene="ptsH"
                     /locus_tag="J1Z28_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBA7715870.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            9081..10808
                     /gene="ptsI"
                     /locus_tag="J1Z28_001016"
     CDS             9081..10808
                     /gene="ptsI"
                     /locus_tag="J1Z28_001016"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBA7715871.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10849..11358
                     /gene="crr"
                     /locus_tag="J1Z28_001017"
     CDS             10849..11358
                     /gene="crr"
                     /locus_tag="J1Z28_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBA7715872.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(11401..12252)
                     /gene="pdxK"
                     /locus_tag="J1Z28_001018"
                     /gene_synonym="thiJ"
     CDS             complement(11401..12252)
                     /gene="pdxK"
                     /locus_tag="J1Z28_001018"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBA7715873.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            12357..12725
                     /locus_tag="J1Z28_001019"
     CDS             12357..12725
                     /locus_tag="J1Z28_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBA7715874.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(12728..13639)
                     /gene="cysM"
                     /locus_tag="J1Z28_001020"
     CDS             complement(12728..13639)
                     /gene="cysM"
                     /locus_tag="J1Z28_001020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBA7715875.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(13774..14871)
                     /gene="cysA"
                     /locus_tag="J1Z28_001021"
     CDS             complement(13774..14871)
                     /gene="cysA"
                     /locus_tag="J1Z28_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBA7715876.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(14861..15736)
                     /gene="cysW"
                     /locus_tag="J1Z28_001022"
     CDS             complement(14861..15736)
                     /gene="cysW"
                     /locus_tag="J1Z28_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBA7715877.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(15736..16569)
                     /gene="cysT"
                     /locus_tag="J1Z28_001023"
     CDS             complement(15736..16569)
                     /gene="cysT"
                     /locus_tag="J1Z28_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBA7715878.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(16569..17585)
                     /locus_tag="J1Z28_001024"
     CDS             complement(16569..17585)
                     /locus_tag="J1Z28_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7715879.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNSEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(17743..18534)
                     /gene="ucpA"
                     /locus_tag="J1Z28_001025"
     CDS             complement(17743..18534)
                     /gene="ucpA"
                     /locus_tag="J1Z28_001025"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBA7715880.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            18814..19710
                     /gene="murQ"
                     /locus_tag="J1Z28_001026"
     CDS             18814..19710
                     /gene="murQ"
                     /locus_tag="J1Z28_001026"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBA7715881.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            19714..21138
                     /gene="murP"
                     /locus_tag="J1Z28_001027"
     CDS             19714..21138
                     /gene="murP"
                     /locus_tag="J1Z28_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBA7715882.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            complement(21316..22215)
                     /gene="yfeX"
                     /locus_tag="J1Z28_001028"
     CDS             complement(21316..22215)
                     /gene="yfeX"
                     /locus_tag="J1Z28_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBA7715883.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(22311..22886)
                     /locus_tag="J1Z28_001029"
     CDS             complement(22311..22886)
                     /locus_tag="J1Z28_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBA7715884.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGVNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(22947..23396)
                     /locus_tag="J1Z28_001030"
     CDS             complement(22947..23396)
                     /locus_tag="J1Z28_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBA7715885.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(23383..23808)
                     /locus_tag="J1Z28_001031"
     CDS             complement(23383..23808)
                     /locus_tag="J1Z28_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase YpeA"
                     /protein_id="HBA7715886.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            24022..24891
                     /gene="amiA"
                     /locus_tag="J1Z28_001032"
     CDS             24022..24891
                     /gene="amiA"
                     /locus_tag="J1Z28_001032"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBA7715887.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            24895..25794
                     /gene="hemF"
                     /locus_tag="J1Z28_001033"
     CDS             24895..25794
                     /gene="hemF"
                     /locus_tag="J1Z28_001033"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBA7715888.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(25800..26852)
                     /gene="eutR"
                     /locus_tag="J1Z28_001034"
     CDS             complement(25800..26852)
                     /gene="eutR"
                     /locus_tag="J1Z28_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBA7715889.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(26898..27398)
                     /gene="eutK"
                     /locus_tag="J1Z28_001035"
     CDS             complement(26898..27398)
                     /gene="eutK"
                     /locus_tag="J1Z28_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBA7715890.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKELD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(27411..28070)
                     /gene="eutL"
                     /locus_tag="J1Z28_001036"
     CDS             complement(27411..28070)
                     /gene="eutL"
                     /locus_tag="J1Z28_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBA7715891.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(28080..28967)
                     /locus_tag="J1Z28_001037"
     CDS             complement(28080..28967)
                     /locus_tag="J1Z28_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBA7715892.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTTCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(28988..30349)
                     /locus_tag="J1Z28_001038"
     CDS             complement(28988..30349)
                     /locus_tag="J1Z28_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBA7715893.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(30361..31764)
                     /gene="eutA"
                     /locus_tag="J1Z28_001039"
     CDS             complement(30361..31764)
                     /gene="eutA"
                     /locus_tag="J1Z28_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBA7715894.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSRSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLCPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(31761..32987)
                     /gene="eutH"
                     /locus_tag="J1Z28_001040"
     CDS             complement(31761..32987)
                     /gene="eutH"
                     /locus_tag="J1Z28_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBA7715895.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGA
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVLIVAVLVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVMKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATAAKTEAEAQS"
     gene            complement(33104..34291)
                     /gene="eutG"
                     /locus_tag="J1Z28_001041"
     CDS             complement(33104..34291)
                     /gene="eutG"
                     /locus_tag="J1Z28_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBA7715896.1"
                     /translation="MQSELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGQSLPKAVGYGHDLVARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRDRFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRMGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(34281..35117)
                     /gene="eutJ"
                     /locus_tag="J1Z28_001042"
     CDS             complement(34281..35117)
                     /gene="eutJ"
                     /locus_tag="J1Z28_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBA7715897.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAALFCKQFPALQVHLPQHSLFMTPLAIASSGREKAEGIY
                     AK"
     gene            complement(35128..36531)
                     /locus_tag="J1Z28_001043"
     CDS             complement(35128..36531)
                     /locus_tag="J1Z28_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBA7715898.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPTATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLKVPEQTRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIVLAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(36543..36830)
                     /gene="eutN"
                     /locus_tag="J1Z28_001044"
     CDS             complement(36543..36830)
                     /gene="eutN"
                     /locus_tag="J1Z28_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBA7715899.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(36937..37230)
                     /gene="eutM"
                     /locus_tag="J1Z28_001045"
     CDS             complement(36937..37230)
                     /gene="eutM"
                     /locus_tag="J1Z28_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBA7715900.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(37269..38285)
                     /gene="pta"
                     /locus_tag="J1Z28_001046"
     CDS             complement(37269..38285)
                     /gene="pta"
                     /locus_tag="J1Z28_001046"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBA7715901.1"
                     /translation="MIIKRCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARTGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(38282..39085)
                     /gene="eutT"
                     /locus_tag="J1Z28_001047"
     CDS             complement(38282..39085)
                     /gene="eutT"
                     /locus_tag="J1Z28_001047"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBA7715902.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLAFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(39082..39783)
                     /gene="eutQ"
                     /locus_tag="J1Z28_001048"
     CDS             complement(39082..39783)
                     /gene="eutQ"
                     /locus_tag="J1Z28_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBA7715903.1"
                     /translation="MKKLITANDIREAHARGELAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASASADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGALQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(39758..40237)
                     /gene="eutP"
                     /locus_tag="J1Z28_001049"
     CDS             complement(39758..40237)
                     /gene="eutP"
                     /locus_tag="J1Z28_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBA7715904.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDNGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(40250..40585)
                     /gene="eutS"
                     /locus_tag="J1Z28_001050"
     CDS             complement(40250..40585)
                     /gene="eutS"
                     /locus_tag="J1Z28_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBA7715905.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
BASE COUNT         9382 a        11410 c        10397 g         9508 t
ORIGIN      
        1 tttttcacta ttaataggct gaaaaaactg tattggtact ctcctttccc ttgcattaac
       61 aatatgccgc ttttaatcaa ccttttatta agcaatcaac taataacgat cggcataggg
      121 aattatgcta tgaaaaaaca tctttttact ctgacactct cttctgtatt ggcgatacca
      181 gcagtatccc atgcagaatt taaaggcggt tttgctgata taggtattca ttatcttgac
      241 tggactagca gaaccactga gaaatcgtcg accaaatcac acaaagatga ttttggatat
      301 ctggaactgg aaggcggcgc taactttagt tggggagaaa tgtacgggtt cttcgactgg
      361 gaaaactttt ataatgatcg tcatgataaa ccgggtagcg agcagcgtta tacatttaaa
      421 aataccaacc gtatttacct gggtgacacc ggatttaatc tctacttgca tgcgtatggc
      481 acctacggtt ctgcgaatcg tgtgaatttc catgacgata tgttccttta cgggatcggc
      541 tacaatttca ccggcagcgg ttggtggttc aaaccgttct ttgcaaaacg ctatacagac
      601 caaacctatt ataccggcga taacggttat gtcgccggtt gggttgcagg ttacagcttt
      661 atgctgggca gtgagaaatt caccctcacc aactggaacg agtacgagtt tgatcgtgat
      721 gccacctacg cggcaggtaa tggcggcaaa gaaggtctga acggtgccgt cgcactttgg
      781 tggaatgcaa cttcacacat caccacaggg attcagtatc gttatgcgga tgacaaactg
      841 ggcgaagatt tttaccagga tgcgataatc tactccatca aatttaattt ctgattgatt
      901 actgccggag ctcccggcag tatgaattca ctctatatct gatacaacgg atccccttcc
      961 cgcccagcca ttagcgcatt gccctggtct cgtactacgt cccataacgc ttcagcggcg
     1021 gtggaaagtg aacggttctt cctgcgcacc agcatcaact ggcgttcaac cactggcgta
     1081 atgcgcttta ccaccagtgg gctaccttca ggcaatggta gcgccagtgc gggaagaata
     1141 ctgatgccga tgcctgcggc taccatcggg aacagcgtcg ccggatgacc aatctcctgc
     1201 acaatattcg cctgaatacc gttgcgcgcc agcgctgcgt caatcagcgg tcggctgcct
     1261 gacgcgtaat cctgcaacac caatttcgct ccctgtaatg cctgccaggg gacataatct
     1321 tccacggcca aagcgctatc gcgatggcaa agcagaaaga aaggctcgga aagaatcact
     1381 tcacattgca gatcgcccac tgggccagga tcgatgacga tgccaaaatc gacatctccc
     1441 tggcgaatac tttccatcac ccactgctgc ggacgatcgt gcaggacaaa ctgaatatct
     1501 ggatagcggc gatggctttc agcaatgcat tgcggaataa gatgcgcgga aatggtctgg
     1561 ctggcagcga cccgaacttt tccgctcagt tgttgcccca tacgcccggt atcgcgcaac
     1621 gtgctgttaa gttcatccag cagtcgctca agacgcaaag ccaactgctg ccctgcatct
     1681 gtaagcacca cttcacgcgt ggttctgtcc agcagtcgaa caccagtatg attttccagc
     1741 tccttcacac tgtgactcac tgccgactgg ctcaggccga tacgctctcc tgcacgacta
     1801 aaacttttct cctgcgctac tgtgacgaaa acctttaatt gctttaaaga atgattcatc
     1861 tgtttttttc atgaatggat gcaataaatc aattttattt ctcaaactgg aagaagcaca
     1921 atagaaccat cgatcatctg gagtctttat gaaacttttt cgtatcctcg atcctttcac
     1981 cttaaccctg atcacggtgg tgttgctggc ctctttcttt ccggccagag gcgatttcgt
     2041 ccccttcttt gaaaatctga ccactgcagc gattgctctg ctgttcttta tgcacggcgc
     2101 gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac tggcgactgc atttgtgggt
     2161 aatgtgcagc accttcgtgc tgtttccgat tctgggcgtg ctgtttgcct ggtggaaacc
     2221 ggtaaatgtc gacccgatgc tctactctgg ttttctctac ttgtgcattc tccccgcgac
     2281 cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt aacgtcgcgg cggcggtttg
     2341 ttctgcgtca gcatccagcc tgctggggat tttcctttca ccattgctgg ttggtctggt
     2401 gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc ggtaaaatta tgctgcaact
     2461 gttgctgcca tttgtgttgg ggcatctttc ccggccttgg attggtgact gggtgtcgcg
     2521 taataaaaaa tggattgcga aaactgacca gacgtccatt ctgttggtgg tttatacggc
     2581 attcagcgaa gccgtcgtta acggtatctg gcacaaagtt ggctggggat cattgctgtt
     2641 tatcgtggtg gtcagttgcg ttcttctggc tatcgtgatt gtagttaacg tctttatggc
     2701 acgccgactg ggcttcaata aggcagatga aattactatc gtcttttgtg gttcgaaaaa
     2761 gagtctggca aatggcatcc cgatggcaaa cattctgttc cccacatcgg tgatcggtat
     2821 gatggtgctg cccctgatga ttttccatca gattcaattg atggtctgtg cggtgctggc
     2881 gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag caggaaagca gcgccgataa
     2941 agcttaaagc ggacgcttca ggggctggac cagttgcgtc agcccctcga ttttaatcag
     3001 cagcgtgatt tgcattagct caccgagctt ccctgccggg aactcatctt tgcgggcaaa
     3061 ccacagcaga tactcctccg gcaggtcgat taagcgacgc cctttgtact tgccaaacgg
     3121 cattatcgta ttggctattt caatcagctg ctctttttcc atctcagcta cccagcaaac
     3181 gcagcatttc cgtttcgtcg atgacttcaa tgcccagttc ctgcgccttc gccagtttag
     3241 atcctgcagc ttcacccgct atcaccagat cggttttctt cgacacgctg cccgcgactt
     3301 tcgcccccag ttcgaccagt cgagctttag cgtcatcacg cgacatctgg cttaagctgc
     3361 ccgtaagcac cacggtttta ccagcaaacg gactgtcaat ctcttccgca ttgataacga
     3421 tcggctcagg ccagtgaaca ccttccgcca acagctcgct gatgacattg cggttgcttt
     3481 cttcggcaaa gaagttgtga acgtgggagg caacgacaat tccaacatca ggcacctttt
     3541 gcagctcttc aatcgacgcg gcttccagcg cttccagcgt gccgaaatat gccgccagac
     3601 ctgctgcggt agcctcgccg acttcacgga tgccaagtgc atagaggaag cgggcaaagg
     3661 tggtttcttt cgctttttcc agcgcgttaa ccacgttttg tgccgatttc ggccccatac
     3721 gctccagtcc ggtcagtttg cctgcggtga gtttgaacag atccgccgga gtgtggacat
     3781 attctttttc aaccagttga tcgatgattt tgtcgcccat tccgtcaaca tccatcgcac
     3841 ggcgggaaac aaagtgtttc agagactctt tacgctgcgc accgcaaatc aggccaccgg
     3901 tacagcgggc aaccgcttca ccttccacac gctcgacgtc agaaccacat accggacaat
     3961 gcgtcgggaa tacaacctca cgggtatctt ccgggcgttc agaaagcacg acgttaacca
     4021 cctgcgggat cacgtcgcca gcgcggcgaa taaccacttt atcgccaatg cgtaaaccaa
     4081 gacgttcgat ttcgtcagca ttatgcagtg tagcgttact caccaacacg ccagcaacat
     4141 ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt acgcccaacc tgaaactcga
     4201 cgtcacgcac aaaggtcatc tgttcctgcg ccgggaattt aaacgctacc gcccagcgcg
     4261 gggcacgcgc gacaaagcca agctgctcct gctgtgccag tgagttgacc ttaatcacca
     4321 cgccgtcgat atcaaagccc agcgtcggac ggtctttttc tacttcgtga taaaacgcca
     4381 gcacttcttc cgccgattca caaagtgtta cccgatcact gacaggtaat ccccacgctt
     4441 taaattgcat taagcgtccc aaatgggtat ccggcaattc accgccttcc agaacaccaa
     4501 cgccgtagca gaagaaagtg agcggtcgct tcgctgtaat acgcggatca agctgacgca
     4561 gtgaaccagc tgccgcatta cgtgggttag caaacacttt cccgcccgtg cgtcgcgcat
     4621 cttcgttaat cttttcgaac cccgcctgcg gcaggaacac ttcaccacgc acttccagac
     4681 gcgccgggat attctcaccg tgtagcttca gcggaatggc gcgaatagta cgcacattag
     4741 acgtgatatc ttccccggtg gtgccatcgc cacgagtcgc ggcactgact aaaacgccat
     4801 tttcatacag aatactgacg gcaagaccat ccagcttcag ctcacagcac caggtgactt
     4861 tctcgttgct tttcagacgg tcctgcacac gtttgttgaa agcaagaaag ctttcttcat
     4921 caaaaacgtt atccagcgac agcattggca cttcatggcg gatctggctg aaagccgcca
     4981 gcggcgctgc acctacacgt tgggtaggcg aatcaggcgt aatcagttct ggatgtttgg
     5041 tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc agcgtcggga atttccggcg
     5101 catccatcac atgataaaga tattcatgat ggcgaagcgt cgttcgcagt tctgtcagtt
     5161 gttgttcgat tgattccata tcgcaccatc aatgctaaaa acccccgaca agcgggggtt
     5221 cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg cgtctttgac ttcgcggatg
     5281 atgtcctggt actcgcgcaa tttctgcgga gtcatcatac ggcgctgatc gtcaagcact
     5341 acaccgccca cttcatcggc aatatgctgc gcagattgca gcatcagctt aaagttctgc
     5401 agctcgtcac cgtaagacgg tacctgcata aagatagtga cacccggagt agtgaaatcc
     5461 ttcatttcag gatcaaaggt tcccggtttc accatattcg ccaggctgaa taacgccggg
     5521 ccgctgccat ccgggctaag atgacgatgg taaatgttca tatcgccaaa aatgaagccc
     5581 gcttgttgaa tgctgttaag aagcagttca ccgtttaact cgctaccgtg atgcgccgcg
     5641 acgttcatga taatcaccac ttctttgcgc ttcggtttat ccataactgg agccggttcc
     5701 gctacaggct caggctgtgg tgccgctacg ggttctgcag gctgaaaagc ctgttgtgcc
     5761 ggttgcggtg ctgaatgcac tggctgcggc gctgacgcga cctgtggcga aactggctgc
     5821 tgcaacggct gttcaggctg cggctgatag gcaggctgct gcacgggttg ctgcaccggc
     5881 tgcgttggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
     5941 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
     6001 gcctcatgct cctgggcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
     6061 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
     6121 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
     6181 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
     6241 tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact
     6301 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
     6361 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
     6421 acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg
     6481 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
     6541 ctcgtcttgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
     6601 ttgatggggg gcgcattctg gtggctcttt acgcaactcg atgtctggat cccgacgctc
     6661 atgagttatg ttccggactg gttacaatgg ctgagctatc tgttgtggcc cctggctgtc
     6721 atctctgtgc tgttagtatt tggctatttc ttttcaacga ttgctaactg gattgccgct
     6781 ccgttcaacg gtttattggc tgaacaactg gaagcaagac tgactggcgc tacaccgcca
     6841 gataccggaa ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
     6901 aaatttgcct ggtatctgcc gcgcgcaatt gtattgctaa ttctttactt catccctggc
     6961 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
     7021 cagtattgtg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
     7081 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg
     7141 atcccgctgc ttaatctgtt cattatgccc gttgccgttt gtggcgcgac ggcgatgtgg
     7201 gtcgattgct atcgcgataa acacgccatg tggcggtaac aatctaccgg ttattttgta
     7261 aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt
     7321 atttcccttc tgcatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
     7381 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
     7441 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
     7501 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
     7561 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
     7621 tgaaccaacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
     7681 caaacttacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
     7741 gttaggtgca aacctggtgc tgaccgaagg tgctaaaggc atgaaaggcg caatccaaaa
     7801 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
     7861 tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga
     7921 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
     7981 ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac
     8041 cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg gtccgcataa
     8101 aattcagggt attggcgctg gttttatccc ggctaacctt gatctcaagc tggtcgacaa
     8161 agtcattggc atcaccaatg aagaagccat ttctaccgca cgtcgtctga tggaagaaga
     8221 gggtattctt gctggtatct cttctggagc agctgttgct gcggcgttga aactacaaga
     8281 agatgaaagc tttaccaata agaatattgt ggttattctg ccgtcatcgg gtgagcgtta
     8341 tttaagcact gcattgtttg ccgatctttt cactgagaaa gaactgcaac agtaatgcca
     8401 gcttgtaaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa
     8461 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
     8521 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
     8581 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
     8641 acccgccagc aatggactgt attacgctct tcgtgcgtcg cgtctgttaa aaactggcgc
     8701 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
     8761 ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg
     8821 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
     8881 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
     8941 ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt
     9001 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
     9061 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
     9121 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
     9181 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
     9241 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
     9301 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
     9361 aagataagca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
     9421 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
     9481 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
     9541 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
     9601 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
     9661 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
     9721 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
     9781 aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
     9841 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa gtgtgcgcta
     9901 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
     9961 tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
    10021 ttgctgctta caaagcagtg gctgaagcgt gtggctctca ggcggttatc gttcgtacca
    10081 tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt
    10141 tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc
    10201 tccgcgctat cctgcgtgcc tctgctttcg gtaaattgcg catcatgttc ccgatgatca
    10261 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
    10321 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
    10381 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
    10441 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    10501 accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    10561 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    10621 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    10681 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    10741 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    10801 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    10861 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    10921 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    10981 aaaatcgttg gtgatggcat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    11041 gacggcacca ttggtaaaat ctttgaaacc aaccacgcgt tctctatcga atctgatagc
    11101 ggcgttgagc tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    11161 aagcgtattg ctgaagaagg tcagcgtgtg aaagttggtg atactgtcat tgaatttgat
    11221 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    11281 gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt
    11341 atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt
    11401 ttatgcttca gccagaggcg gcaaaatcaa ttcatcgctc tcatgctgct gggtatagcg
    11461 catcacttcc agtacgcgca accccgcgcg gtgtaccgca tcggttaacg ccttcccttt
    11521 cagcaaacca ctgataagct gagcacaaaa caggtcgcca gtccctttca ggtcagtttt
    11581 aacccgggaa tgggaaatga cattcacgct gtcggcactg accaccacca cctgcatctc
    11641 ctgattttct tcattgccgg aggcgctggt aatcaccacc cactttaatg tgtctgaaag
    11701 cagacttttt gcggcagcaa tggcactgtc gagatcgcgg caatctttac cggtcaggat
    11761 ttccagctca aagatattgg gggtaattcc ctgtgccagc ggcagtaaat attgtcgata
    11821 cgcctcggga aggtcaggtt tgacataaat tccgctatca atatcgccaa tcaccggatc
    11881 gaccatgatc aataggtcag gatggtcttt gcgtagcgca gtcagccact cggcaaggat
    11941 tttgatttgc gatgccgttc ccatatagcc cgtggttaca gcacgaagtt ggcgcagcgc
    12001 atcacgctcc tgaagcgcac gcaaataacc gctaaaccat tcatccggaa tcgcaccacc
    12061 gtagaaagtg tcataatgcg gcgtattgct cagcaatacc gtcggcacgg caaagacatt
    12121 caggccgttc tgtttgatag caggcaccgc aatgctgttg cctacgctgc cgtacactac
    12181 ctgcgactgc acggcaacga tatccgcctg cagtgccctg ctcttatcgt taaacaacaa
    12241 caaactactc atttaatttt ttctccttgc cgatgatcct catcgtattc cagccgaaac
    12301 tttacctgat tctggcagtc aaatcggcta ttacaaaaca aggataaggt aattcagtga
    12361 agaaaatcat ttgtctggtc gttacgctgc taatgacact ccccgcttat gcgaagttaa
    12421 cggcccatga agaagcccgt atcaacgcca tgctggaggg attagcacag aaaaaggatt
    12481 tgatatttgt gcgcaacggt gatgaacata cctgcgatga agcggtttct catctgcgcc
    12541 tgaagctggg caatacccgt aatcgcattg atacagccga gcagtttatt gacaaggtgg
    12601 cctcgtcgtc atcgattacc gggaagccgt atatcgtgaa gatgcccggt aagagcgatg
    12661 agaacgcaca gcctttttta catgcgttaa ttgcgcagac ggataaaacg gtgcctgcgc
    12721 aataatctta aatccccgcc ccctggctaa aatgctcttc cccaaacacc ccggtagaaa
    12781 ggtagcgatc gccacgatcg cagatgatcg ccaccaccac cgcgccaggg ttagcttttg
    12841 ccacccgcag tgctccggca accgcgccgc cggagcttac gccgcagaaa atcccttccc
    12901 gcactgccag ttcgcgcatg gtgttttccg catcgcgctg atgaatatcc agcacctcat
    12961 ccaccagaga agcattgaaa atccccggca gatattccgc aggccagcgg cgaatgccgg
    13021 gaatactgct gccttcttcc ggctgcaggc caacaatggt caccggtttg gattgttcgc
    13081 gcataaagcg tgacacgccg gtgatagtgc cggttgttcc catgctggag acaaaatggg
    13141 taatacgccc gccagtttgc tgccagattt ccggcccggt agtggtgtaa tgggcgtaag
    13201 ggttatcggg attattgaac tgatcgagca actttccttc gccgcgattc gccatctcca
    13261 gcgccagatc gcgtgcgcct tccatgccct gttctttggt gaccagaatc agttctgcac
    13321 cgtaggcacg catcgccgca cggcgttcct ggctcatgtt gtcgggcatc agcaatttca
    13381 tgcgatagcc tttaagcgcc gcaatcattg ccagcgcaat gccggtgtta ccactggtgg
    13441 cttcgatcag cacatcaccc ggtttaatct cgccgcgctt ttccgcctcg acaatcatcg
    13501 aaagcgccgc acggtctttt accgatcccg ccgggttatt gccttccagt tttaaccaca
    13561 cttcactgcc gttatccggc gccattcgct gcaacttcac cagaggcgta ttgcctattg
    13621 tttgttctaa tgtactcacg atctctgtcc atacgtggtg ttgcctgatg cgacgcttac
    13681 gcgtcttatc agacctacag gttacaaaac cttgccatta aaaaacccgg atcgcgggaa
    13741 gcgcctccgg gcgtttaaca ttcactcaac ctatcaggcg ctttgtgcga gagcaagttc
    13801 ctcatcgcgg gtttcaatac gctcatcgcc gttatacagc cgcgcatgtt gcagaccaac
    13861 gtataaacga tcgccacgct gcggggcatc gtcgccatgc atcacgaccg tcagcggttc
    13921 gttgtaccac cccagcggct gcaccactaa ttgggtgtag tgacctttcg ggctggcttc
    13981 cagtacctgt acgggtagcg gcgaatcgag gctggtacgg cggctgatat ccacttccca
    14041 cgggcgcagg aagagatcca ccggcccctg atacgcgggg gtgtatccca gcggccagcg
    14101 atgtgcgcca acgtggaact gcccgccgcg aatggttccc tgcaggcggt ttacctcgcc
    14161 cataaactcc agcacaaaac gagtcgccgg ttcacgccat acctgatccg gtgcgtcagc
    14221 ttgttcaata ttgccctgac tcatcaccac tacacgatca gctacttcgg tcgcctcttc
    14281 ctggtcgtgg gtcacgaaca cgctggtgaa tttcagttct tcatgcagtt gacgcagcca
    14341 gcgacgcagc tctttacgca cctgcgcatc cagcgcgcca aacggttcat caagcagcaa
    14401 aatttgcggt tccacagcca gcgcgcgtgc aagcgccaca cgctgtttct gaccgccgga
    14461 aagctgcgcc ggataacgat ccgccagatg agcaagctgc accatctcca gcaattttgt
    14521 cactttcgct ttgatcgccg ccgcattcgg gcgttcacgc cgtggcaaca ccgtcaggcc
    14581 aaaagcgata ttgtcaaaca ccgtcatatg gcgaaacagc gcgtaatgct ggaacacgaa
    14641 accgacttta cggtcccgcg cgtgcagacg gctcacgtcg gtaccgtgaa agcgaatatg
    14701 cccactggtt tgatgctcca gcccggcgat aatgcgcagc agcgtagttt ttccggaacc
    14761 ggacggccca agcaacgcga ccatctgacc agaaggaata tccagtgaga tatcgttcag
    14821 cacctgggtg cgaccaaaag acttcttaat attggcaatc tcaatgctca tgatgttcct
    14881 cctgctgcgc gcgtttttcc tgattttcca ggcgccactg caacatactc tttaaaaaca
    14941 gggtgataat cgccatcagc gtcaacaacg cggcggcggt aaaggagccg acggtgttgt
    15001 agtcctgctc cagcaattca atctgtaacg gcagcgacag ggtttcgcca cgaatcgagc
    15061 cggaaaccac cgataccgcg ccaaactcgc caatcgcgcg ggcgttggtc agcaccacgc
    15121 cgtaaagcag cgcccagcgg atgttcggca gcgtgacgcg acggaacatc tgccagccgg
    15181 acgcgccaag taaaatcgcc gcttcgtctt cctggctgcc ctgacttaac attaccggta
    15241 ccagttcgcg caccacaaac ggacacgtca cgaagatggt gaccagcacc atacccggcc
    15301 aggagaacat aatttgcaga ttatgttcat ccagccagcc gcccagcggg ccgttagagc
    15361 cgtagaacag cagatacacc agaccagcaa ccaccggcga cacggcaaac ggaatgtcca
    15421 gcagcgtcag caatagctgg cgtccgggga agttaaagcg cgtcaccagc caggccagca
    15481 gaataccgaa caccaggttt accggtacgg caatcagcgc gatcatcacc gtcagccaga
    15541 tggcgtgcag catgtccgga tcggccagat tttgtaacac cggcatcagt cctttgctga
    15601 acgcctgtac gaagatataa atcatcggca ccagcaggat gaacgccgat accagcatcc
    15661 cgatgccaat cagaaaccat ttgccccagt taatcgggcg cgcgtcataa cgcttcaatt
    15721 gtgtcacttc cgccattaat gacctaccac acgccgacca aagcgacttt gcagagtgtt
    15781 aattgagaac agcaacagca gcgatgccgc gaggatcacc gaggcaatcg cgctcgccgc
    15841 cgggtaatca aactcctgta agcgaacaaa gatcatcagc gacgtcactt ctgttttcca
    15901 ggcgatattc ccggcgataa aaatcaccgc gccaaactca ccgaggctgc gggtaaacga
    15961 cagcgccaca cccgccacca gcgccggaga tagttccggc agcaccactt tgcgaaaact
    16021 ctgccagcgt gttgcgccga gcgtttctgc cgcctcttca tattccgggc ctaactcttc
    16081 cagcaccggc tgcacggtgc gcaccacaaa cggaatgctg gtaaaggcca tcgccaccgc
    16141 aatccccagc caggtatagg tgactttgat atcaaacttc gccagccatt caccgtaaaa
    16201 accgttcacg gaaaagagcg aggccagcgt taaaccggcg actgccgttg gcagcgcaaa
    16261 gggtaaatcc atcagcgcat caagcagcgt gcgacccgga aagcgatagc gggttaagat
    16321 ccacgccatc agcagaccga aaacgccgtt aaaaatcgat gccacaaacg ccgacagcag
    16381 cgtcacttta taggctgcga ccacctgcgg gttggtgatc acctcccagt actgcgccca
    16441 gctcatctgg gccagttgca tcaccagcgc ggagagcggc agcagcaaaa tcaggcacac
    16501 aaacagcaga ctggtgccga ggcttaaggt aaagcccggc agcacgcgtc ttgaagaaac
    16561 tgcaaacatt acttacgccc cgccgctaac agcttgtcta actcgccgcc gctggtgaag
    16621 tgggttttca tcacttccgg ccaggagcca aatttatctt ccacgcggaa cagctcggtc
    16681 tgcgggaatt tatctttcag tttgcccatg acttccgagt tatttacgcg gtagtaatag
    16741 tcggtgatga tggtttgcgc ctgcgggctg tagagccagt tcaggtaggc ttttgccgct
    16801 ttttccgtgc cgttggcctg tacattttta tcgacccacg ccaccgggaa ttccgccaga
    16861 atgttggttt tcggaatcac cacttcaaag ccctgcgctt catactgttt acggatgttg
    16921 ttcacttccg actcgaagct gatcagcaca tcgcccaggc cgcgctcggc aaaagtggtg
    16981 gtcgcgccac gaccgccagt atcgaacact tcaacgtttt tcaggaactg ggtcataaac
    17041 tgttcggttt tggctttatc accaccgtcg gctttgtccg ctgcgcccca tgccgcaagg
    17101 taggtataac gcgcgttacc cgacgttttc gggttcggga aaatcagctt cacgtcagag
    17161 cgtaccaggt cgttccagtc gtggatattc ttcgggttgc ccttacgcac caggaagccc
    17221 atggtggagt agaacggcga gctattattc ggcaggcgcg actgccagtc cgctgggatc
    17281 agcttgcctt tatcgtgcag gatttgtacg tcggtcacct ggttataagt gacaacgtcg
    17341 gcttttaaac cctgcaaaat tgccagcgcc tgttttgatg atccggcatg agattgttta
    17401 atcgtcagtt tgtcgccacc gttatctttt gcccattgtt gctcaaacgg cgggttcagg
    17461 gcggcaaaca gctcgcggga gacgtcataa gaactgttca gcagttccgt tgcctgtaca
    17521 tggcccgcca gtagcagaga agcgaccagc acgagtgagt tctttttcag taagttaacg
    17581 gccattgcgc acccttataa atttaacgac tttctaatag ccatcatatt tataacggac
    17641 gtgaaaggag taacggtttt atataccgtt tggtgatttg gaagttgaaa agggaataag
    17701 acgaaatcct ccccacaaat gcagggagga aacagaggtg aatcagatgc cgacgctaac
    17761 cgtctccggc agtgtgctgc cgccatcaat cacattctgt gtaccggtta aatagctgga
    17821 ttcatccgat gcgaggaagg ccgccagttc gccgacttcc agcggatcgg cgaggcgacg
    17881 catcgggatt gctttcgcca tttcagtcag caccgattct ggatcttccg ggttcgactg
    17941 gcgggcaatg ctttccgcca ttggcgtgcg aacgtatcca gggcagatgg cgttaacgcg
    18001 aataccagac tgcgcgtact ccaccgccag cgatttagtc aggccaacaa tcgccgcttt
    18061 cgttaaggcg tacgccgttt cgcccgggtc tgccaccata tcgccagtga ctgaagacat
    18121 catcacaatc cggccatctt tgcgggcaat catctccggc agcaccgcct tcgtgacgtt
    18181 ccatacgcct tttatattga tatcaatatg gaaatcgcga tcttcatcgc tcatatcaag
    18241 gaaactgccc agacgacaaa cgcctgcgtt attcaccaga atatcaatgc gcccttcttt
    18301 ttccttagcg cgcttaatag ctgcggctac cgacgccggg tcacgcacat cggcgacaac
    18361 cgccgtacag cgatgaccac gaccacacag ttcgtccgcc agcttttcga tctcagggga
    18421 gatatccagc aagattaggt ttgcgccatg acgcgcaaaa gttctggcaa ttccttcgcc
    18481 aattccctgc aatgcgcccg taatcagtgc tgtcttgccc gtgagtttac ccattttaat
    18541 gctctccttg ttaatacaga gcgttacact ccattccctc tgaaaataca ggataaacaa
    18601 aatattgctg cggatcagct cactaattca ggaaaatctc aatttagtcg ctgctttatg
    18661 cagcacctca agcgttacgt cagcgcgcaa tcattttcag tgacatcaca tgagtaatat
    18721 tcggacttaa tgattccttt gtgtctatca aatcgggata tatgatccca tttatctgca
    18781 attatttgat cgcaaatatt aaggtcacca ccgatgcaac ttgaaaagat gattaccgaa
    18841 ggctcgaacg ccgcctcggc tgaaattgac cgcgtatcaa cgctggaaat gtgccggatt
    18901 atcaacgatg aagataaaac cgtaccactc gccgttgagc gcgtactgcc ggatatcgcc
    18961 gcggcgatcg atgttatcca cacccaggta agcggcggcg gccgtctgat ttatctcggt
    19021 gcgggaacat ccggtcgtct ggggattctg gatgccagcg aatgtccgcc gacctacggc
    19081 gtgaaaccgg gcctggtagt tggtttgatt gctggcggcg aatatgccat tcagcacgcg
    19141 gtggaaggcg cagaagatag ccgggaaggt ggtgttaatg atctgaaaaa tatcggctta
    19201 accgcgcagg atgtggtggt tggtattgcg gccagcggac gcacgccgta tgtgattgcc
    19261 gggctggaat acgcgcgcca actcggctgc cgtacagtgg gcatttcctg taatccgggg
    19321 agtgccgttt catccaccgc agagtttgct attacgccgg tagttggtgc cgaagttgtt
    19381 accggttctt cgcgaatgaa agcaggcacg gcgcagaaac tggtgctcaa catgctctcc
    19441 accgggctga tgattaaatc cggcaaagtg ttcggcaacc tgatggtcga tgtggtcgcc
    19501 accaacgaaa aactgcatgt gcgccaggtc aatatcgtca aaaacgctac cggatgtaat
    19561 gccgaacaag cggaagcggc gttaattgct tgcgagcgca actgtaaaac ggccattgtg
    19621 atggtactga aaaatctcga tgccgatgaa gctaaaaaat gcctggatca acacggcggt
    19681 tttattcgca aggctctgga aaaggaataa cccatggcta aagagatcag cagtgaactt
    19741 ctgaacacca ttcttacccg ggtcggcgga ccgggaaata tcgccagttg tggtaactgt
    19801 atgacgcgcc tgcgtctggg tgtacatgac agttcattgg ttgatcccga tatcaaaacg
    19861 ctggaaggcg tgaagggcgt cattttgacc agcgatcagg tgcaggtggt ttttgggcca
    19921 ggcaaagcgc atcgtgccgc aaaagccatg agcgagctgc tgggcgaggc accggtacag
    19981 gatgccgcag aaatcgccgc ccagaataaa cgccagttaa aagccagaca aacttccggc
    20041 gtgcaacaat ttctcgccaa attcgccacc atcttcacgc cactgatccc cggttttatt
    20101 gccgctggtc tgctgctggg gatagcgacg ttaattgcca cggtgatgca cgttccggca
    20161 gatgctcaag gcacgctacc cgatgcgctg aattttatga aggtgttcag caaaggcctg
    20221 ttcaccttcc tggtcattct ggtgggctat aacgcggccc aggcatttgg tggcacgggc
    20281 gtaaatggcg caattatcgc tgcgctcttt ttgcttggtt ataacccaac tgcaaccacg
    20341 ggttactacg ccggatttca cgatttcttt ggtctgccca tcgatccgcg cggcaatatt
    20401 atcggcgtgt tgatcgccgc gtgggcctgc gcccgcatcg aatgcatggt gcgccgcttt
    20461 atgccggacg acctcgacat gctgttgacc tcattaatca ccctgctgat caccgctacg
    20521 cttgcgtacc tgatcattat gccgctgggc ggctggctgt ttgaaggcat gtcgtggctg
    20581 tttatgcacc tgaacagtaa tccgctcggt tgtgcggttt tagccgggct gttcctgatc
    20641 gccgtggtat ttggcgtgca tcagggcttt attcctgttt atctggcgtt aatggacagc
    20701 cagggattca acagcttatt tcccatcctt tcaatggcag gcgcaggcca ggtgggcgcg
    20761 gcgctggcgc tctactggcg ggcgcaaccg cacagtgcat tacgcagtca ggtccgcggg
    20821 gcgattattc ccggtctgct tggcgttggc gaaccgctga tttacggtgt caccctgccc
    20881 cgcatgaaac cgtttgttac cgcctgttta ggcggcgcgg cgggcggttt gtttatcggg
    20941 ctgatagcct ggtggggtct gccgatgggc ttaaacagcg cctttgggcc gtctggtctg
    21001 gtggcgctgc cgctgatgac ttccgcgcaa ggcatccttc cggcaatggc ggtttacgct
    21061 ggcggtattc tggtggcatg ggttagtggg tttattttca ccacactctt tggctgccgt
    21121 aacgtcaatc tggactgatt atgaaacgga caatgctcta tctttctctg ctggctatta
    21181 gctgtagcgt cagcgccaca aaataccctg ttctgacaga aagtagttga tcaagtgtat
    21241 gcggcgttaa agcagtaata aaaaggccag tcagttgtgt tactggctgg cctttaatca
    21301 atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggcgcaa agtaatagcc
    21361 gccggttacc ggtttggtga aacgcaacat cgcatcacgc ttaccatcgg tatcgccaaa
    21421 catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc agaagtacag
    21481 accgtgagtg ccactggcgg tgccgtacgg caggctctga cgaacaatct tcagcccttt
    21541 gccatcttct ttcagatcaa cgcgggtcag gtgagaggtt tccggacgtt cgtcgccgtc
    21601 aatctcttcg ttggcctctt tggtacgccc gatcatcatc tcttgatcgt gaacgctcat
    21661 ccgattgagc tgtttcaggt tgtgctccca acgctgcaca aacacatagc tgccgcccgc
    21721 atccacgccg tctttgataa cagccacttc gcggcgcgtc tcttcacccg ccgggttttc
    21781 tgtaccgtca acaaagccgc tcaggtcacg ctcttcaacc caacggaagc cgtggatctc
    21841 ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga cagagaagtt
    21901 tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg tcggcgcaag
    21961 gcctttaccg tagcccggaa aatctttcag ctcttcggcc ccaacgccgc cgctcagagc
    22021 gcgccaggta ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg ggaatttcgc
    22081 ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg cgtcaacttc
    22141 ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca aaatgccact
    22201 ctgaacctga gacattgttc ctcctgaaaa taataatgcc acgcaatccg tggcattgtt
    22261 gggcgctatt ttacgtgttt tttcgcggcg gcggtttgtt cagacgcaaa ttaacgccgc
    22321 cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat taacccttca
    22381 gggccactcc attccccgct gaactggtag ctaatatgtt gactgccttc ggctttacat
    22441 tcgacggcac gattatcatc actgtcggcc ttttggcaat tgccaaatgc tttgctgtaa
    22501 aggtcactaa acggtgtacc gattttaaca ccggtgtcag caggaatatc gctatccagc
    22561 acatcaatgc ggctgatcgt gccttgatca ccgttaatca ccatcgccac gttgacgcct
    22621 ttcatcactt caaaaaagcg caccacgttg ccgttcgcgg ttttcattcc gctgcgcagg
    22681 cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt ggacgccgtt
    22741 aattcaccca cgccctgctc gctcactttg gtggatgatc caaaccagtt ccacgggtta
    22801 gcggccgacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa cggcatagcg
    22861 cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata acgttgcttg
    22921 gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca agcgcgtaaa
    22981 cgtcgattgg tcagcagcca gattaacgcc acgatatccg ccactaccag cgccagaccg
    23041 ataccgctaa cggattcacc gttcagccac agccacggtt gccaacaaag taaaaccact
    23101 tgcgccaaca gcaacagaaa atagagcaca tgccagatac ggggaaatga agcccgccga
    23161 ccactcagca aaaatgccag caccgccgga ataccgggaa tcaaccccag ccagaaatta
    23221 tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcgcgcga cgctccggca
    23281 atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa gagcaagggt
    23341 aaacgcaggc gaccgtgcgc atcataatgg acaggatgaa actcagtact cttcatcttc
    23401 aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc gttcatacat
    23461 tccgagcacc atgtcgttat cttccggcac gttgatctga attttcggac agccgcgagc
    23521 aatcagcttt ttctccagcc gattaagcaa cgcattagca atcccacgcc cacgaaactc
    23581 gggatgcacg ccaagataat aagcagaccc gcgatgccca tcataaccgc ccatcaccgt
    23641 tccgaccact tcaccgttca cctcagcgac cagaaacaaa ctgacgtcat ggttcatctt
    23701 acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac gctcccaaag
    23761 ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg tattcgttac
    23821 ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca atatctgacg
    23881 aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg aaaagtaaaa
    23941 caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca tctggaactt
    24001 tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact cacttcgcgc
    24061 cgccaggtgc tgaaagccgg tttggctgcc ctgacgttgt caggaatgtc gcaagccatc
    24121 gccaaagaag aaccgttaaa aaccagtaac ggacacagca agccgaaagc aaaaaaaagc
    24181 ggcggcagac gtgtcgttgt tctcgatcct ggccacggcg ggattgatac cggagcgatc
    24241 ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcaa ttgccaagaa tgtccgttcc
    24301 atattgcgta atcatggcat tgatgcgcgt ttaacgcgtt ctggcgatac gtttatccca
    24361 ctgtacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat gtcaattcat
    24421 gccgacggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct ctctaaccgc
    24481 ggtgcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc cgatgaagtt
    24541 gccggtaaaa aagcgactga caaggatcac ctattacagc aagtgctgtt tgatctggtg
    24601 caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa gaagattaag
    24661 ccggtgcata aactgcacag ccgcaatacc gaacaagccg cgtttgtggt gttgaaatcg
    24721 ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccaga agaagagcgg
    24781 ctgttaggca ctgcggcgtt tcgccagaaa atcgccacag cgattgccga aggcgtgatc
    24841 agttatttcc actggtttga caaccagaaa gcacactcga gaaagcggta agtgatgaaa
    24901 cccgacgcac accaggttaa acagttcctg ctcaaccttc aggatacgat ttgtcagcag
    24961 ttgagcgccg tcgatggcgc agaatttgtc gaagatagct ggcagcgcga agctggcggc
    25021 ggcgggcgta gtcgggtttt gcgtaatggt ggtgttttcg aacaggcagg cgtcaacttt
    25081 tcacatgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgtcc ggaacttgcc
    25141 gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa tccgtatgtc
    25201 cccaccagcc acgcgaatgt gcgctttttt attgccgaaa agccgggtgc agagcccgtc
    25261 tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga agacgccatt
    25321 cactggcacc gcaccgcccg tgacctgtgc ctgccatttg gcgaagacgt ttatccgcgt
    25381 tacaaaaaat ggtgcgacga atacttctac ctcaaacatc gtaacgaaca gcgcggcatt
    25441 ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt tgcctttatg
    25501 caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg acgaaaagcg
    25561 atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg ttatgtcgag
    25621 ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg gcgcaccgag
    25681 tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca gccaaaagat
    25741 ggcagcccgg aagcggcttt aagtgagttt attaaggtca gggattgggt gtaactccct
    25801 cacccccact ctcgcatccg ctgatgcagc gtcagtgacg gcttctcggc aaacagttgc
    25861 tgataatccg tggcaaattg cccaagatgc cagaatcccc actgcatggc ggcgtctttt
    25921 accgttgtgc tttgtgacca cggacttatg agttcgcggc gcacggcgtt caggcgaata
    25981 cgtttcagcc acgcgttcgg accaatgccc aaaatggcgt gaaacgcgtt ttgtagcgtg
    26041 cggcggctga catgcagttg attacacaag tccagcaccg tcaccggttc ggacatgttt
    26101 tccagcacat attcacgggc gcgggaaagc aatcgacggt aactctgatg actgatgctt
    26161 tccgccgtca ccattggctg cgcttcttcc agcatcgccc ccatcgccat tagcaaatta
    26221 tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc gcaaaacgtc
    26281 gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt cacttccagc
    26341 gccgactggc tacgcagcat atgtaatacc cgatccggat tatgcaaaaa gttagcctga
    26401 cgagtgatga catcttcaga aagcactacg ccaaggatcg tgtaatcgtc cggcgtactt
    26461 aattcaaact cggttccacc agggcgcgtc gcaatttctg cgcttcccag acattgcgaa
    26521 ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga attcggccag
    26581 accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg aatatcatcg
    26641 agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc gtaaatctgc
    26701 tgccaggcgg taattgtcaa agcatgttca tagacatccg ttgtctgtcg ttgatgaaca
    26761 ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc atgataaaga
    26821 tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt tgtaggacac
    26881 ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg tttacgtaac
    26941 gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg ccagccaaag
    27001 tgggctgcca cttctcctgc cgtcattccc tgacgtactg atgttaacag cgccaacaac
    27061 tcgtcagcag attccgcgac gataactggc gcgtcgagtt cctttacggg ttgcttaggc
    27121 tggcggttaa agccagtaac cagccactgg gtgtcatcgt ctggccgacc gatctccttg
    27181 cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag cgccgcacga
    27241 cacgccgcca gatcgccttc caccaccagc gttaggcgac cagggtcaag cacttcgtga
    27301 ctgagcagac gaacgttagc tgctttgagc atggcatccg cagcatctat cgcggcgacc
    27361 atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg ttacgcacgc
    27421 tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca ggctgcttta
    27481 cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga aggcggcggg
    27541 acataggtca ccagctgcac gtcggcagat ttcaacgctg catcaatgcc gtaggtcgct
    27601 tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc ggcggttgat
    27661 gagagataag aaccggtacg cgaaactaca tgtgccagga aggctgtgtt ttctgcgtca
    27721 ttagcccact ggaaagcagc gccattttca atatgcgcaa ccatcgcatc cagaccagca
    27781 cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc agtcggtgac
    27841 gggccgtgtg cggctcccgc atacagcgag cggccataca ccacttccac catcgcctgc
    27901 ttggtcgctt cgtcagccgc aatgtaggtg acgtcatctg aatcagcaga aatcagcccg
    27961 aggctacgaa tatgcggcgg caatttcagt tcacgcgcaa attcggcgtt aacggaggca
    28021 atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat gatgcctcct
    28081 tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc caaatccaca
    28141 atgacggctg cggcttcaac cggcggcgtg ccgccctggt gaatgttaga gatacaggta
    28201 cggtcggcct caacggtggt tgccatacgc ggagagtaaa cggcgtagca ggagaggctt
    28261 tctgactgcc ctaatcccgg gcgttccccc accagtagga tcactacttt cgcgccgagg
    28321 atctcaccga tctgatcttc aatcttcacg cgaccataac gaacaaagaa tggcgtaccg
    28381 actttcagcc cggcctgttt caggcccgcc atcagcggtg gcaggatctc ttcgtagttc
    28441 acggtgatcg catcagttga caggccatca gaaataacga cctgtacgtc cggattagca
    28501 acacactgtg ctttcagcgc ttcaacagct tctgcacaca ggcggcggcc catatccgga
    28561 cgagtcaggt agagattttt gtcgctaatt tcagagcgga cttccagcaa gccctgcgct
    28621 ttcacccact cttccggcac ttctttcagt acggtatctt tcgaacgaga gtgatcggcc
    28681 aggaaacgca gcaacgcctg ggtacgcgga cgcggaccgg cacgaccggt acaaacgcgg
    28741 gccacggtgc tgcgacgcag ttctgttaat acgtctgcgc gatgcgggtt ttcaacgcca
    28801 atccacgctt ttgcttccgc agaacctaaa tccagcgcgc agctttccga ggtcaccggt
    28861 gccgcacagg tggtggtggc gcactttgct tctgacggtg cgggggccgc ttgtcccatt
    28921 gacgccatca cgctgcgtac aatttcttca atctgttttt gatccatgat gtgttatctc
    28981 cgcgtcatca gaagaacagt gacggatcgc ccgcccgttt ggtcaggcga ccgtttgcca
    29041 taatgcccat gctttccagc cagcgttcaa actccggtga cggacgcaga ttcagtaact
    29101 gacgcacagt ggcagtgtcg tggaatgccg tggtctgata gttgagcatg atgtcatcgc
    29161 ccagcggcat ccccatgatg tagttgcagc ctgcggtggc gagcaggatc atgaggtttt
    29221 cgttgaggtt ctggtcagcg tcagcgtggt tggtgtagca gcagtcacag cccatagaga
    29281 tgccgctcag cttacccata aagtgatctt ctaagcccgc acggataatc tggcggtcgt
    29341 tgtagagata ctccggccca ataaagccga ccacggtgtt gacgataaac ggatcgtaat
    29401 gacgcgccag cccatagtta cgtgcttcca tcgtcacctg gtctgcgccg aagttagcgc
    29461 cagcggacag cgcagagcct tgtccggttt cgaagtagag gcagttttcc ccggcgatac
    29521 ggttgaactc cgcgcccact gcgcgcgctt catcgagcat cgccagctct acgccaaact
    29581 ctttcagccc tttttcgctg ccgcagatac tctggaaaat cagcccgcct ggtgcgccac
    29641 gacggatcgc ttcgatctgg gtggtgacgt gcgccagtac gcagccctga gttgggatgt
    29701 tgaatttgtc gatcacgccg tagatggtgt ccagcacgcg gcttaagttt tccacgtcat
    29761 ccgtcaccgg gttgacgccg atcaccgcat cgcccacccc gaaggaaagc ccttcgtaga
    29821 tttgcgcagc gatactttgc acgtcgtcac gggtgtcatt tggctgcaaa cgggcgctga
    29881 aggtgcccgg aataccgatg gtggtattgg cctttttgat taccggcatt ttcttcgcgc
    29941 cgtagatcag gtccgcgttg gagcaaatct tcgctaccgc cgcgaccact tccgaggtca
    30001 gccctttgcg ggtaaaggca atgtcgtcca cgctggtttc atcgctcagc acatactcac
    30061 gcagttcgct gatgctccag tttttaatct ggttgtaggc cgtttcgtta acatcgtcct
    30121 gaatcagccg cgtcacgcag tcatcttcat aggcaatcac cggattattg cggatgtccg
    30181 ctacggtcat ttccgacaac acctgctttg ccgccacgcg ctcctgtgaa cttgccgctg
    30241 caacgcctgc cagcacatcc cccgaacgca gttcgttggc tttagccagc acctctttta
    30301 catccttaaa ctgatataca ttgccgaaca atgtggtctt tagtttcata agtcgttccc
    30361 tcaggaagga aatgcgagtg atttcaccgt caccggcaca accgatccgc caaaaagagg
    30421 cgtaccaatg tcgatatagt cccccgcgcg gacaatcact tcgtcaatga ctgccaacgg
    30481 gagttgttgt agctgtgggc gcaacaacat gcccagagct ttaccaaagt cctgcccggc
    30541 caccaccagc aggggatgcg gattcggaaa acgcgcgacg aaatcgacca gcgcgttgat
    30601 gaccgtcagt accgcggcgt aacgcacagg cagcgaggcg ggaagcgcca gcacgtacgc
    30661 gtcagttttg gggcaaagat ccagctgaat cagcgcctgt tgccaggcac tcaccagatc
    30721 cgtttcatca atcgggatcg ccaccggcaa attgcgcagc ggcagttgta cgccctccag
    30781 ccagatcgtg ctgccagaga gtgaaagggt atgtgcaccc gcaccaatca ccgtggcgcg
    30841 cacggtttgc gccggaaact gcacgttcat ctcacgcagg cgcggatggt catgcagcgc
    30901 cgttgccaga agcgggccga tatcggcaaa acagaacggg tcggcgggct ggtggcgata
    30961 acattcgccc acgccgccag aaagcgtaat gatttcgggc gtaacacctg cgggcagcaa
    31021 accggtttgc attaatgcct gcgcgagcgg cgagagcgtt ccgtcaatca cttcgacaat
    31081 cagctctgcc atccgccggg taacctgcac cagctgcgcg ccggtcagcg aacgggcgtc
    31141 agtgcctgca ccgaagcact catccactat catctgcccc ggtttatgag cgtaaaccac
    31201 gcgcccctgg ctgtcggttt ccagcaggcg accgccgacg ttgaggcagg cagtgccgct
    31261 gatttttccg gcatcgaaca gggcgtagtt cgcggtgcca ccgccgatat cgatattcag
    31321 gacccgacac agccgttgtt cagaaagggt ttgcgccccg gcaccgtgtc cggcgatcac
    31381 ggattcaaga tgcggcccgg cgctggcgac gacaaaatcg cccagcgacc gggagagcgc
    31441 catcaccgcc gggcgagcgt tgcgggtttt tgcgctttca ccggtgataa tgatggcacc
    31501 agaatcaacg ctttccggcg caatgcccgc agcctgatat tgctcgagga ttaaggtttt
    31561 cagttccgct tcttttaaac caccctgttt atcgacaggg gtaaagaaca ccgggctttg
    31621 ccagctaatt tcgcgtttga tgaattcgta gcgcggcacc tgcgacaccg ccgcacggtt
    31681 aaccagctcc aggtgggaga agatcacctg ggtggtggtg gtgccgatat cgataccgac
    31741 gctcagtagc tggcgagtgt tcacgattgc gcctccgctt cagttttagc cgcggtcgcg
    31801 tcttcttttg gcaccagcat catcgccacg ccaattgccg ttacgccgcc gatcaatttg
    31861 ccgacaatca tcgggaagat catggcgttc atgttggcag cggcgaagcc taaatggtcg
    31921 cccagggcga aagcagcgga aacggcgaag gcgcagttga tgactttgcc gcgggtatcc
    31981 atctgcttca tcatgccaaa catcggaatg ttgttggcaa gcgttgccac catgccggct
    32041 gccgcgatat tgttcatatt cagcacttta ccgacactca tcagcggttt ttcaaaccag
    32101 cgagtcagca gcagtaccat cggatacgcc cctaacagaa cgcaggagat cgagccgata
    32161 acttcaatgg cgcgcatcac ttcaccgggt ttatcgccag gggccataaa gataggatca
    32221 agacccggga tcagttccca gccaaggagg aatttcatta ccgcagcggc aagaccgagg
    32281 gtgatcaatg caacgaggaa tttggcgaag atctggaagc cgttgatcat tttttccggg
    32341 atgaatttca gccccagcgc caccagcacc gcaacgataa gtaccgggat catgttcatc
    32401 aggatcagcg caaaggtgaa ttcaacaggc tggccgttga tctgcacacc ggagtacatc
    32461 gctaccagac caccagcgat acagccaatc ggaatggtca ctatgcccgc cagcacgccg
    32521 agcgccagat aacgacggtc agaaggttcg ataatgccga gcgccactgg aatggaaaac
    32581 acaatcgttg gccccatcat cgacccgaga attaacccag agtatagcca cgctgctacg
    32641 tcaccgcccg ccagctcttt ggcgaggaag aagccgccca tatcgcacgc cagcagtgtt
    32701 ccggcgaaca ttgacgggtt tgcgccgagc atttcgtaaa ccgggataat caccggcccg
    32761 agtacgtggg ccagtaccgg tgccagcgcg gtcataccga ccatcgccag gcccagagcg
    32821 cccattgcca taaagccttc ttcaaactgg ccgccggccc cttcgatact tttgccgaac
    32881 ttaccgagga aacgagcaga accgccgaac tgcgacagga tcctgtctac ggcagctatc
    32941 agcataaaga acatcatgat gtacatgatg atttcgttaa ttcccatcgc ctttactccc
    33001 tgttagttgt tatttattgg cggatgcggc gtgaacgcct tatccgccct acatgtgcaa
    33061 tcccgaaggc cagataagac gcggcaagcg tcgcatccgg catttattgc gccgctgcgt
    33121 acaggccgac aatctgctcg aggctggcgg tacgcgggtt actgcgcaga caaatatctt
    33181 ccagcgcggc ctgtgcccat gcgccgtaat gcgcagatgt cgcaccaaca tcgcccattc
    33241 gtttaccaat cccaacttcc gctatcagct cacttaccgc gttaatagcg tcacgatcgt
    33301 cggatttttt agttcgcagc gcccgaccaa tctgactaaa ccgatcacga cagaccatcc
    33361 ggttgaactc catcaccgtt ggcaacaaca tggcgttcgc cagaccgtgc ggaatatgca
    33421 gcgccgcccc tggctgatgc gccatcgcgt ggcataaccc aagccccgcg ctggaaaacg
    33481 ccattcccgc catacatgaa gccagcaaca tgctctcgcg cgcgacaagg tcgtgaccgt
    33541 agcccaccgc tttcggcagc gattgaccaa tcatcgcaat ggcaccaatc gccaggctgt
    33601 cggtaaacgg tgtagcgttc agcgcgctgt acgcttcaat ggcatgggtt aacgcatcaa
    33661 tgccagtcat cgccgtgaca tgcgacggca cgccttcggt caacgcggcg tcgagaatcg
    33721 ccacatccgg catcagcgag gcatgggcta acacctgttt gcgcccgctt accgcatcga
    33781 taatcaccgt cacgttagtc gtttcagagc cggttccggc ggtcgttgga atggcaatca
    33841 acggcaggcg cggttgcaga acgctggttt ctgacatttc tgccagcgtg ctatccgggt
    33901 tcgtcaccag caacgccacg gctttcgccg catccagcac cgagccgccg ccaaacgcga
    33961 tcaccccgtc acaacctgat tcacgcaact gcgccacagc tgcacacacg tcggtgatgc
    34021 acggttcgcc caccggacat ggccagagcg tcatggcgat acctttaacg gccaggctac
    34081 gtgtcagccc ggcggtcatc cctgcctgat gcaaaaagct gtctgccatc acgaacagat
    34141 gtttcaaccc acgcgtttgc gcttgctgcc cgcaactgct caccgcgccc ggaccgcaaa
    34201 gcgtcaccgg tggaacgcta aatgttttta cccgttgcag attcagggta tcgaacgcct
    34261 gaaaaagcgc cgtttgcaat tcactttgca tagatcccct ccgctttctc tcgcccacta
    34321 ctggcgattg ccagcggggt cataaacaaa ctgtgctgcg gtaaatgcac ctgtaacgcc
    34381 ggaaattgtt tgcaaaacag cgccgctacg cccggttgca tacaggaacc gcccgccagc
    34441 cataaatccg taatgccctg cccttcaata tgccgggcca caatgtccgc cattttttcg
    34501 tacaccggtt tcaccgcggg ccaaatctcg tcaccgtgac cgcgtttgta ctgctctgcc
    34561 tcttccagcg aaatacggcg atttccggcg agagtcagag agatgtgatg tccgccggtg
    34621 gcttcatccg ccgagtacgt cactttgccc tttttcacga tggcaatgcc ggtagtgccg
    34681 ccgccgatat ccactacacc ggcgttgtcc agttgcagca gatccgcaac tgcggttggc
    34741 tcatccagca catggctcac ttccagtccg gcagactcca gcacgttgat ggaaatacgc
    34801 gggtccgtac caggaggaaa tgaggtcgcc gcatggctaa aacggcgacc gaattgctgc
    34861 tcaagggtgt cgagatggcg acgaacaatg gtgaccgcac cgaagaaatc ccagacgatg
    34921 ccgtcgcgga ccacatcggc ccagtcgagg cataccgcca ccggctgacc gtcgcggtcg
    34981 acaaccatcg acaccacatc gcaagtgccc agatccaccc ccagccacag cggagattcc
    35041 gtcgcggcag gcgtctggtt acacagcgtt gccgccgttt gcagacgtgg ggtgagccat
    35101 tgttcatcgt gcgccatcgg ttactcctta aacaatgcga aacgcatcga ctaatacaca
    35161 gcgacgcaga cggacaaacg tccgcgcgct ggttacccct tcaccggttg gcgtggtgat
    35221 ggtcatcgtg gtccagcctt ccccgcccag ccccagcccg gcaatacacg gtccgttctt
    35281 aacgaaaatg ctggtatcaa tggcgttcgc catctggttc atgttttcga tattgcgcga
    35341 gtgcattgcc gccgtgtggt ggcaaccgcc ttccagtttc accgccagca caatggcatc
    35401 cgccacgttg gcgacgcgca cgacgggcag caccggcatc atcagttcgg tcacggcaaa
    35461 cggatgttct gcggtggttt ctacaaacag cagtcgcgtt tgttccggaa ctttaaggcc
    35521 gattgccgcc gcgattttcg ccgcatcgcg accaacccag tcacggctga cggtgccttt
    35581 tccgcgctcg tcgatatttt tcagcaacac cggttgcagt tgctgcgcct gctctgcggt
    35641 cagtttcacc gcatgctggc cttccatcag acgcatcagt tcatcggcta cgctatccac
    35701 aacaatcagt accttttcgt cggcacaaat gatgttgtta tcgaaagaag cgcctttgac
    35761 gatggactga gctgcacggg cgaggtcggc ggtttcatcc accactaccg gcgggttgcc
    35821 agcgcctgcg gcaatcagac gtttattggt gtgtttacgc gccgcttcca ctaccgcttc
    35881 gccgccggtc accaccagca ggccgatacc cggaaacttg aacaagcgtt gggcggtttc
    35941 gatatccgga tttgctacgg taaccagtaa gttttccggc ccgcctgcgg caacaatcgc
    36001 ctggttgagc agcgtaatcg cccgctggga gacttttttc gccgccggat gcggggcaaa
    36061 aatgacgctg ttgcccgcgg caatcaggct gatggcgttg ttaattacgg ttgccgtcgg
    36121 gttagtggaa ggcgtcaccg aagccactac gccccagggt gcgttttcaa ttaaagtcag
    36181 gccgttgtcg ccagtcagca cctgtggaga gaggcactca acgcctggtg tgccgcgcgc
    36241 ctgagcgacg tttttggcaa atttatcttc aacgcgcccc atgccggttt cactgacggc
    36301 aagttccgct aaatctctgg cgtgtttttc gcctgcttca cgaatggcag caatggctaa
    36361 ctggcgcatt gccacgcttt ttaacccttg ctgggcgact ttggctgccg caacggcgtc
    36421 atccagggac gcgaaaacgc ccatctcatg aacggcggcg ggcggcgtgt cactgctttg
    36481 cattttcagc agtaccgctt tcaccacctg ttcaatatcc tgttgattca tgatgttctg
    36541 ccttatttgt ggaaaatgac ctgaccgcca gacaccacct catcgacgat gccaatcacg
    36601 cacagatcga ccggtgacgt ttcgctttta tgcgcctggc gggcggaact gccactcacc
    36661 agtaacaccc actccccggt tcccgcgcca atattgtcga tggcgacggc gcattgcccg
    36721 tcaggattac cttgtggatc aatcatttcc accatcagca atttgtcatg cgccagtccg
    36781 tgatggcgta cggtacaaac aatttgtcca gtgacgactg ccagtttcat acccgcctcc
    36841 gtggcgtatt tcaggtaaaa gctcccccta ccctccgcag aaggtaaaat gaaaaaggag
    36901 agagcgtgac gcccgaatcg acgtcacaca gggtgattac aggttgctgc tatcgccttt
    36961 caggccgatc gggaagactt cttccaggtc gccgtgcgga cgcgggataa cgtgtacaga
    37021 taccagctcg ccgatacgct gtgccgctgc ggcacctgca tcggtcgcgg ctttacaggc
    37081 tgcaacatca ccacgaacca tggcagtaca caggccgccg ccaatctgct taacaccaac
    37141 cagcttgacg cgcgcagctt taaccatcgc gtcagaagcc tcaatcagtg caaccaggcc
    37201 ccgggtttcg atcattccta atgcttccat tgtgctttcc tctttatcag ggtccagaac
    37261 gggaccgttc attcaaccag tgtttgtaaa ctgctttcgc ggttcacttc tgtctgacgc
    37321 ggcacagctg ccaccagcgc cagttcgata atttcctgca cgctacaacc acgagagaga
    37381 tcgtgcatcg gcgcggcaag tccttgtatc aatggcccga cggcacgata tccgccgagt
    37441 cgttgtgcga ttttgtagcc aatatttcct gcctccagcg acggaaaaac catcacatta
    37501 gctttgccct gtaacgggct ggcaggcgct ttttgcgccg ccacttccgg cacgaaggcg
    37561 gcgtcaaact gcaactcgcc atcgacaacc agctttggtg cgcgctcacg gacgatttct
    37621 gtcgcctgct ggacgttggc aacgcagggg tgacgggcac taccgttgct ggaaaacgac
    37681 agcatcgcca cgcgcggttc ttctccggtg atggcgcgcc aggtttcggc gctggcaagc
    37741 gcgatatccg ccagttgtgc cgccgtcggc tgcggcacca cgctgcaatc ggcaaagccc
    37801 aacgccggac cgctgtactg tggcagcatc aggaaaatgg atgagagcgt tttacagcct
    37861 ggctgcaagc cgataatgcg taatccggca cgcagcacat tcgccgtgga agagaggttg
    37921 cccgcgatac agacatccgc tttaccggcg ctgaccattg cggcggcgaa catcagcgga
    37981 tcggtaagtt tttccagcgc atccggcggc gttttttcgc ccgtgcgggc cagccagcga
    38041 tgagcaaatt cttcccgcat tgcgaggttg ccatgcggat ctatcacctg tagcccgtcc
    38101 atcgccacgc cgtgactgag cgcaaactga cgaagttcaa acggattggc gaccagaatg
    38161 ggcgttgcca gaccttgttg atgtaaatat tgcgcagctt tcagcacacg ttgatctaac
    38221 gcatccggaa aaactactct ggcgggcgct cgcaacgcca gttcacgaca acgtttaata
    38281 atcattgcgt ttcccccagt cgttgttgga tctgcttcac cgttagcggc tgcttcgtta
    38341 cgctcaggat catcatcacg tagaccgtgc tggaaaggcg gttcagtgcc tgcaaaatgt
    38401 ccgggcgtaa tacttcaaag ctgcgggtga taaagacctg cgccgcgacg gtttcggttt
    38461 cccgcacttt ggtgcgtaac agattcagca acgccgcatc gcgcccgtgg ctggcttccg
    38521 gcaccagatg atcgtggtcg agataacgca gcggctggtg agaaagccga tgcagatctt
    38581 cgtcagaaag cccgacaatc gcctgacagc ccaacggttc ccccagcgca tcggcacgca
    38641 taatgttgcc aagacgcgaa cggatatccg ccagccacgg ctgccacggt tccgccagtt
    38701 caatttgcag ccacactgcc agcgcaatgg tgctgtcgag aacagcgcga aaagccaggc
    38761 gcggatcgct tttggcaacc attttttccg ccgacaggtg agtcagcgta tccggctttt
    38821 ttgccaccgg ctggcggcac agttcgcagc acgcctgcgg atgttcatcg ctactggtca
    38881 gcccgtgaac gggctgcggc tgctgctgtt catcgtcaac aaacaagcgg ccctgctcgt
    38941 caataaactt gatgcgcaga tggcggcttt ccagtaactc ccgggcagag ggcgtcaggc
    39001 gactgtccgc agggagatgg atctctgcgc cttcgctgag cgtatggttc gctcttagcc
    39061 atgcttcggt gatgaaatct ttcataggga ttgccagtta gccggccagg cgacatacag
    39121 gaatttcacg ctggatgtcg taccaaattc gatactggag cctttcggga taaacatcac
    39181 atcgcccgct ttggcgatca tagtttcgcc ttcgtggcga acatgcagct ccccttccag
    39241 caccatgtcg atttcgtcgt agttcagggt ccacggaaag aaggcgttct cccactgcat
    39301 gaaaccagcg gccatactgc tgccgtcgtc tccggtaacg agatcagtca agcctacgca
    39361 gtgcggttcc gcgccatcga aacgaccaaa cttgacgctg ctgccatcaa taactttgat
    39421 gccgcctttg ccggtcaccg atttaaagct cggctgcagt gcaccctgtt ccagcgactg
    39481 tttttccttc atcacttttt ccatcagctg cgcgaccagg ctttcggtaa actgaccttc
    39541 cggcagttgg gcgataatgg tttcacggat gcgctggctt tcggttttat cggctgatgc
    39601 gctggcggga acagacgccg ttaccgggat cgattcatca cattcggtga tggtaaaacc
    39661 caacagatca gccacttcac gagcttccgg ggtaataatg ctggcgcgca gaaccacgga
    39721 cattgccagt tcgccgcgcg cgtgtgcttc acgaatatca ttcgctgtga tgagtttttt
    39781 cacctgcttc ctcctgtttg gtgagagatg ccagataatc caccagttgc tgcacgcttt
    39841 gcgggtcgtg gctgtttagc tcgaaaatcg gctcttcgaa gcccgtctca agcaataact
    39901 ttcgcgttgc ggcgacatca gcatctggca tgtccgtttt actgatgacg gcgatttgcc
    39961 gtttgctgac gccaatatcc agcaacccgg caggcaaccg actttccggg tcattcgcgc
    40021 cgtggacata gatcagcatg tccacatcct gtagcgtggt aattaaggcg tgataccagc
    40081 ggggatggct aaaatattca cccggcgtat caatatcgcc attatcatta aattccacgg
    40141 cctgtgtttt tcttgcgagg gtataattcc cctgcaacgc attaaataac gttgtttttc
    40201 ctgccccgac ggtacccaca aacgcaattc gtttcatgaa tgcctcatat taacttttgg
    40261 tcatttcaca aagcgtataa tttaataagc gtcctaaacc actgacggtt tgtgataacg
    40321 cttcttctac tgcgcccacc gaaccataaa tcaccagcgc gccgctaaaa cggtcgagaa
    40381 aaccgatatg cacatcggcg gctttcaacg ctaaatcacc ggcgatcatc gccgtttctc
    40441 cgggagttag cgtcataatg ccgattgcgc ccgcatccgg aacgccgatt ttcttcgcca
    40501 gttcctcgcc gggatgcgcg atgagatgcg ccagtgtgac ctgtttaccg ggcacaaatt
    40561 cctgaatgat gcgttcttta tccatgagtc gccgcctgtg aaaaactttc gtgtatcgtg
    40621 acaatttttc gtcagagtaa ataacaaaat tcggcaagcg cgtttaaaag gtgaggtaga
    40681 tcactaagaa tggagag
//
LOCUS       DADSZD010000022        39081 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.022, whole genome shotgun sequence.
ACCESSION   DADSZD010000022 DADSZD010000000
VERSION     DADSZD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39081
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.022"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            265..924
                     /locus_tag="J1Z28_001051"
     CDS             265..924
                     /locus_tag="J1Z28_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA7715906.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            921..1682
                     /gene="pphC"
                     /locus_tag="J1Z28_001052"
     CDS             921..1682
                     /gene="pphC"
                     /locus_tag="J1Z28_001052"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBA7715907.1"
                     /translation="MSWRLVYASTVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELWLNDVLATDIVLTIRQRLFAEAEA
                     KELAVRDFSCTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYTNMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            1679..3619
                     /gene="yegI"
                     /locus_tag="J1Z28_001053"
     CDS             1679..3619
                     /gene="yegI"
                     /locus_tag="J1Z28_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBA7715908.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHILFGGR
                     HPYSGVPLISDAGNALETDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRPLPSGLLRREYI
                     ILIEIALSALSLLFCGLQAEPSYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRARLEKMKDEILGLPEEEKRALVTLHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKIAAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(3632..4984)
                     /gene="yegD"
                     /locus_tag="J1Z28_001054"
     CDS             complement(3632..4984)
                     /gene="yegD"
                     /locus_tag="J1Z28_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA7715909.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSNANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            5118..5966
                     /gene="alkA"
                     /locus_tag="J1Z28_001055"
     CDS             5118..5966
                     /gene="alkA"
                     /locus_tag="J1Z28_001055"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBA7715910.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYAHSLTVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNLQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            complement(6076..9393)
                     /locus_tag="J1Z28_001056"
     CDS             complement(6076..9393)
                     /locus_tag="J1Z28_001056"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7715911.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHSERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFQQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLIDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNSSYFAIN"
     gene            9711..10352
                     /gene="udk"
                     /locus_tag="J1Z28_001057"
     CDS             9711..10352
                     /gene="udk"
                     /locus_tag="J1Z28_001057"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBA7715912.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            10444..11025
                     /gene="dcd"
                     /locus_tag="J1Z28_001058"
     CDS             10444..11025
                     /gene="dcd"
                     /locus_tag="J1Z28_001058"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBA7715913.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            11047..12900
                     /gene="asmA"
                     /locus_tag="J1Z28_001059"
     CDS             11047..12900
                     /gene="asmA"
                     /locus_tag="J1Z28_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBA7715914.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTLSLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTSNDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(12968..14551)
                     /locus_tag="J1Z28_001060"
     CDS             complement(12968..14551)
                     /locus_tag="J1Z28_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA7715915.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGEMEYEV"
     gene            15209..16348
                     /locus_tag="J1Z28_001061"
     CDS             15209..16348
                     /locus_tag="J1Z28_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBA7715916.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            16354..16797
                     /gene="wzb"
                     /locus_tag="J1Z28_001062"
     CDS             16354..16797
                     /gene="wzb"
                     /locus_tag="J1Z28_001062"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBA7715917.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFTAVYTLLERSARQWAQALNAEQV"
     gene            16800..18962
                     /gene="wzc"
                     /locus_tag="J1Z28_001063"
     CDS             16800..18962
                     /gene="wzc"
                     /locus_tag="J1Z28_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBA7715918.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMADLVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            19055..19894
                     /gene="wcaA"
                     /locus_tag="J1Z28_001064"
     CDS             19055..19894
                     /gene="wcaA"
                     /locus_tag="J1Z28_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBA7715919.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            19897..20385
                     /gene="wcaB"
                     /locus_tag="J1Z28_001065"
     CDS             19897..20385
                     /gene="wcaB"
                     /locus_tag="J1Z28_001065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBA7715920.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            20382..21599
                     /gene="wcaC"
                     /locus_tag="J1Z28_001066"
     CDS             20382..21599
                     /gene="wcaC"
                     /locus_tag="J1Z28_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBA7715921.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLLNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALSDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSVLNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            21574..22791
                     /gene="wcaD"
                     /locus_tag="J1Z28_001067"
     CDS             21574..22791
                     /gene="wcaD"
                     /locus_tag="J1Z28_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBA7715922.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWCAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            22802..23548
                     /gene="wcaE"
                     /locus_tag="J1Z28_001068"
     CDS             22802..23548
                     /gene="wcaE"
                     /locus_tag="J1Z28_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBA7715923.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIEMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            23564..24112
                     /gene="wcaF"
                     /locus_tag="J1Z28_001069"
     CDS             23564..24112
                     /gene="wcaF"
                     /locus_tag="J1Z28_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBA7715924.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRKRVETE"
     gene            24139..25260
                     /gene="gmd"
                     /locus_tag="J1Z28_001070"
     CDS             24139..25260
                     /gene="gmd"
                     /locus_tag="J1Z28_001070"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBA7715925.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            25263..26228
                     /gene="fcl"
                     /locus_tag="J1Z28_001071"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             25263..26228
                     /gene="fcl"
                     /locus_tag="J1Z28_001071"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBA7715926.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTRPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            26231..26710
                     /locus_tag="J1Z28_001072"
     CDS             26231..26710
                     /locus_tag="J1Z28_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBA7715927.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDEMLEAAFERLTMAELGLRLPITAGQFYGVWQHFYGDNFSGTEFTTHY
                     VVLGFRIRVAEDELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLSEKRAGVPGL"
     gene            26707..27930
                     /gene="wcaI"
                     /locus_tag="J1Z28_001073"
     CDS             26707..27930
                     /gene="wcaI"
                     /locus_tag="J1Z28_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBA7715928.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAASSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNRAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            27933..29369
                     /gene="cpsB"
                     /locus_tag="J1Z28_001074"
     CDS             27933..29369
                     /gene="cpsB"
                     /locus_tag="J1Z28_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBA7715929.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            29474..30844
                     /gene="cpsG"
                     /locus_tag="J1Z28_001075"
     CDS             29474..30844
                     /gene="cpsG"
                     /locus_tag="J1Z28_001075"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HBA7715930.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINFKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            30899..32293
                     /gene="wcaJ"
                     /locus_tag="J1Z28_001076"
     CDS             30899..32293
                     /gene="wcaJ"
                     /locus_tag="J1Z28_001076"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBA7715931.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRIEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            32295..33773
                     /gene="wzxC"
                     /locus_tag="J1Z28_001077"
     CDS             32295..33773
                     /gene="wzxC"
                     /locus_tag="J1Z28_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M-antigen undecaprenyl disphosphate flippase"
                     /protein_id="HBA7715932.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDLLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWSSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIIIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLAVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            33846..35126
                     /gene="wcaK"
                     /locus_tag="J1Z28_001078"
     CDS             33846..35126
                     /gene="wcaK"
                     /locus_tag="J1Z28_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBA7715933.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEEF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACALTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNTRLNEAVSRERQTGMQMVKSVLERIGEAK"
     gene            35123..36343
                     /gene="wcaL"
                     /locus_tag="J1Z28_001079"
     CDS             35123..36343
                     /gene="wcaL"
                     /locus_tag="J1Z28_001079"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBA7715934.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVAIPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVIKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            36354..37748
                     /gene="wcaM"
                     /locus_tag="J1Z28_001080"
     CDS             36354..37748
                     /gene="wcaM"
                     /locus_tag="J1Z28_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBA7715935.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALSARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPGGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMRGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEVKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGMFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            37923..38816
                     /gene="galF"
                     /locus_tag="J1Z28_001081"
     CDS             37923..38816
                     /gene="galF"
                     /locus_tag="J1Z28_001081"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBA7715936.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         9550 a         9833 c        10508 g         9190 t
ORIGIN      
        1 tactcatcat cgtggtgctc ttagtcatta gctggccagc ttactaagac taccagggcg
       61 ggggaaaccc cgctctaccc tcactcctga aattatgtca ctccgccctt agtgtcaatc
      121 gacggctctc cgttcttgcg aggcgaatag caaaattttc tgcgccgccg ggcctaattc
      181 tgaaagtttc ttcgtttgct cttatttcat tattgcaatc ggtatgatac cggacctcaa
      241 taattctcta agaaaggatt tcttatgagc gaacaaatca cgtttgccac cagcgatttt
      301 gccagtaacc cggaaccacg ttgcccctgc attttgctgc tggatgtttc tggctcgatg
      361 agcggcagac ccattaatga acttaacacc ggattggtta cctttcgcga tgagctgctt
      421 gccgattcgc tggccttaaa aagagtggaa ctcggcattg tgacgttcgg cccggtgcat
      481 gtggaacaac cctttaccag tgccgctaat tttttcccgc ccatcctgtt tgcccagggc
      541 gatacaccaa tgggcgctgc cattaccaaa gccctggata tggttgagga gcggaaacgt
      601 gaatatcgtg ccaatggtat ttcctattat cgtccgtgga ttttcctgat caccgatggc
      661 gcaccaaccg atgagtggca ggctgccgcc aacaaagtgt ttcaggggga agaagataag
      721 aagtttgcct tctttaccat cggtgttcag ggggcggata tgaagacgct ggcccaaatc
      781 agcgtccgtc agcctttgtc tctgcaggga ttacaattcc gtgaactgtt tagctggtta
      841 tccagttcgc tgcgttcggt ttcccgctcc acgccgggaa cggaagttgt gctggaagcg
      901 ccaaaaggct ggacgtcagt gtgagctggc gtctggtcta tgcatcaacc gttggaacgt
      961 cgcacatcag cgcggacttg ccttgccagg atgcctgcca gatgcaggtt gcctggttaa
     1021 atgaccagca gccattattg tcggtatttg tcgctgatgg cgcaggcagt gtctcgcagg
     1081 gtggcgaagg cgcgatgctc gccgtcaatg aagcgatggc gtatatgtcg caaaaagtgc
     1141 agggcgggga attgtggctt aatgatgtcc tcgccacgga tattgtgctg accattcgtc
     1201 aacgactttt tgccgaggcg gaagccaaag aattagcggt tcgcgatttt tcctgcacgt
     1261 ttttgggatt gatttcatca gctaatggca ccttgattat gcagattggc gatggcggcg
     1321 tggtggtaga tttggggcac ggtttgcaac tgccgctcac gccgatggtg ggtgaatata
     1381 ccaatatgac gcacttcatt accgatgaag atgccgtttc cagactggaa actttcacca
     1441 gcactgagcg tgcgcacaaa gttgcagcat ttacggatgg tattcagcga ctggcgttaa
     1501 atatgctgga taattctccg catgtgcctt tctttacccc gtttttcaat ggactggcgt
     1561 cagcaacgca ggaacaactc gatttattac ctgaattgtt aaagcagttt ttgtccagcc
     1621 cggcggtaaa cgagcgtacg gatgatgata aaacgttggc actggcgctg tggttaccat
     1681 gaaacccact ttatatactg ctactggtga gtgcgttacg cctggccgtg aactgggcaa
     1741 aggcggcgaa ggtgcggttt atgatatcga ggagtttgtc gatagcgtcg ccaagattta
     1801 tcacacgccg ccacccgcct taaaacagga caaacttgcc tttatggctg cgacagctga
     1861 cgcgcagttg ttgaattatg tcgcctggcc gcaggcaacg cttcacggtg gacgaggcgg
     1921 aaaagttatc ggttttatga tgccaaaagt ttctggtaaa gaaccgattc atatgatcta
     1981 tagcccggca catcgtcgcc agagttaccc tcattgtgcg tgggattttc tactctatgt
     2041 tgcgcgcaat attgcttcat cttttgctac tgttcatgag cacgggcacg tcgtgggtga
     2101 cgtaaaccag aacagcttta tggtaggtcg cgacagcaaa gtggtgttga ttgatagcga
     2161 ctcctttcag attaatgcca atggtacact gcatttatgc gaagtcggcg tgtcgcattt
     2221 tacgccgcca gagctgcaaa ccatgccatc atttgtcggc tttgaacgca cagaaaatca
     2281 cgataatttt ggccttgcgt tgctgatatt tcacatcttg tttggtgggc ggcatcctta
     2341 ttccggtgta ccgcttatct ctgatgcagg taatgcgctg gagacggata ttgcccattt
     2401 ccgttatgcc tacgcctcag ataatcagcg acgtggttta aaaccgccgc cacgatcgat
     2461 tccgctgtcg atgttaccgg gcgatgttga agccatgttt cagcaggcat tcacggaaag
     2521 tggcgtggca accgggcgtc cgacggcaaa agcgtgggta gcggcacttg atttactacg
     2581 ccaacagtta aagaaatgta ccgtttcggc aatgcatgtt taccccgctc atttgactga
     2641 ctgcccgtgg tgtgcgctgg ataatcaagg cgttatctat tttattgatc tcggtgaaga
     2701 ggtcattacc accggcggtg attttgtgct ggcgagagtc tgggcgatgg tgatggcatc
     2761 agtagcaccg ccagcattgc aattgccatt acccgatcat ttccaaccga ctggcaggcc
     2821 gcttccttca ggcctgttac ggcgcgaata catcattctg attgagatcg cactgtcagc
     2881 gttatcgctg ttgttttgcg gccttcaggc agaaccgagt tatattattt tgattcctgt
     2941 gctggcggct atctggatta ttggcagcct gacaagcaaa gcgtacaaag cagaaatcca
     3001 gcaacgccgt gaggcgttta atcgcgcgaa aatggactat gaccatttag tcagccagat
     3061 ccagcagttg ggcgggctgg aaggttttat cgccaaacgg gcgaggctcg aaaaaatgaa
     3121 ggacgaaatt ctcgggttac cggaagaaga gaaacgcgct ctggtaacac ttcacgacac
     3181 cgcaagggaa cggcagaagc agaagtttct ggagggattt tttattgatg ttgcctctat
     3241 tcccggcgtt ggccctgcgc gtaaagcggc gttacggtcc tttggtattg aaacagcagc
     3301 ggatgttacc cgacggagcg ttaagcaagt aaaaggtttt ggtgatcatc tgacccaggc
     3361 ggttatcgac tggaaagcga gctgtgaacg ccgttttgtg ttcaggccga acgaagcggt
     3421 aacgcctgca gacagacaag cggtaatggc aaaaatagcc gccaaacgac atcggctgga
     3481 atcggcgctg actgtcggtg cgacagagtt gcagcgattc cgccttcatg cgccagcacg
     3541 gaccatgccg ttgatggaac cgctacgtca ggcggcagaa aaactggctc aggcgcaggc
     3601 agatttaagc cgttgctgag ccatttttca attaacgaaa caccacttcc gcccagcgtg
     3661 ccagcccggc ggtgacggag ccaaagtcat cgccgcctgc aatcggaatg cccggcaact
     3721 gttctgccag cgctttttta atcagcggcg aacgggcgct accgccggtc aggtagataa
     3781 cgtccggttt ttcctgggcg ttatccagcg ccagttgcac ctgttccaga atccgcgcca
     3841 gtggctggct gagggcgctt tccagccctt gctggctaat cagcgtagcc agttcatcgc
     3901 tgataaacgg cagtgaggcg cgggtttccg ctacacttga aagggcgatt ttgctctctt
     3961 ctgcgctacg caccaggcga tagcttaaac gctgacgcca gactttctgt aacagggcca
     4021 ctttttccgg ttcacgggca tcgcgtacca gatcgttaag cagacgaccg ttagcattac
     4081 tgtagaaatc actctgcgca ggaacgtcgt tgatcgcaac cgcattccac cacggcagaa
     4141 tcggcagggc gatgcctttt tcggtttcgc cacccatgcc cagtaacggc atcaggttct
     4201 taaacgccag tgcgatatcc agatcgttac caccaatacg gcaaccacta tgacccagca
     4261 ggctggcttc acgatcgaga cgcgaacgcc attgcggccc catcagcagc aatgaacagt
     4321 cagtcgtacc accgccgata tccactacca gcactcgttt ttcttcctgc aaggtggctt
     4381 cgtaatccag cccagccgcg accggctcgt actggaatac cacatcctta aacccggcac
     4441 gcttcgccgc gcgttccaga atcccttgcg cctgggtgtt tgcttcatca ccgcccagcc
     4501 cctggaagtt gatcgggcga ccaatcaccg cctgagtaat tgcctccggc agctgcgcct
     4561 gcgcctgttg gcgaatgtgc aacatcattg cgcagaccag atcctcaaac agcgctacct
     4621 gctgcggttt taagccgctg gcaccgagga acgattttgg tgatttcaca aaccacactt
     4681 cttctggatc atcaatgtac tgtgccagtg aggaaagacc gaactgcacg cttttcgccg
     4741 taacatcgat atcttcttcg cggttataac gaatcgcccg acgcaacagc gcctgcgttt
     4801 catcgtcgtc tgccggaaca tcatgatggc ggtacagcca ttcgcttaca gcttcacgcg
     4861 ttggcgcgca aagcattgaa ggcagcagcg tgctgtcgtt ttccattttt agcaaatgcg
     4921 gtttaccgtc gcgcatgacc gccactgaac agtttgctgt accgtaatca aaaccaataa
     4981 acacgaaata atccccatgc cggtgaagaa ggggcgtgac tttagcgaaa tgttgccgtc
     5041 gcgacaaccg gaatatgaaa gcaaagcgta acgtctgaat aacgtttatg ctgaaagcgg
     5101 attaacaagg agatgcgatg tataccctga actggcagcc gccgtatgac tggtcgtgga
     5161 tgttgggatt tctcgccgcc cgtgcggtga gcggtgttga aacggttgcg gacgattatt
     5221 atgcccatag tctgacggtg ggcgaatatc gcggcgtggt gactgctatt ccggatatag
     5281 cccgccatac tctgcacata aatttaagtg caggtttaga acctgttgcc gctgagtgtc
     5341 tggcgaaaat gagccgcctg tttgatctgc aatgtaacct gcagattgtt aacggtgcgt
     5401 tgggcaagtt aggcgcggcg cggccaggat tgcgtttacc tggttgtgtt gacgcttttg
     5461 agcagggcgt gcgggcgatt ttaggccaac tggtgagcgt ggcgatggcg gcaaaattga
     5521 cctccagagt ggcacagctt tatggcgaac ggctggatga ttttccggat tatgtctgct
     5581 tccccacgcc tcagcggctg gcagtagccg acccgcaggc attaaaagcg ttaggtatgc
     5641 cgttgaaacg ggcagaggcg ctgattcatc tggcaaatgc ggcgctggag ggcaccttac
     5701 caatgacaat accgggcgat gtagaacaag cgatgaaaac gctgcaaacc tttccgggta
     5761 tcgggcgctg gacggcaaat tattttgctt tgcgtggctg gcaggcgaaa gatgtttttc
     5821 tgccggatga ttatctgatt aaacagcgat ttccgggaat gacaccggca caaatccgcc
     5881 gttatgccga gcgctggaaa ccctggcgtt cttatgcgct gttgcatatc tggtatacgg
     5941 aaggctggca accagacgaa gcatgacgtt ggttgccgga tgcggtgcga gcgccttatc
     6001 cggcctgggg aatgagcgat ttgtaggcct gataagacgc gttagcgtcg catcaggcaa
     6061 tgggcaaacg ctcccttagt taatcgcgaa ataactgcta ttcaccagca aatccagcgg
     6121 ttgggcattg gcaatcacat caccataaat cagatcgacg ccgatgcccg aaagcgtatc
     6181 catcactaag ggtaaaacga ctggcccggc gatagttttc atcccgagtc gctgagcgtg
     6241 cccctgaata atcgtaatca gcatctcatc tatcaaatta ccctgcacgt tggcgcataa
     6301 ctcaccatca agtagcaggt aatctgccat atttgctttc agcgagttga agatttgcag
     6361 atctcggccc acctgactga atacgatccg acatcccgcc agccgcagtt tttgcacgct
     6421 ttctgcgtga tcgaaaatcg cttctgccgg aataatcaga tgtaataacc gtgctggtag
     6481 agggctattt tccagctgct caagcagttc attcaccagc gtggcgctac tcaaaccagc
     6541 aacggaaagg gggagggcga tgcttaagcc tttactggca accgcttttg cggcctgctg
     6601 gaaaaaatcg tggcataccc gttggtcaag agcatggcta agtgccggat cgctgaagct
     6661 acgacgaaat gtttgttcat caataatctc gctttcgcaa ctccagagct taagtgaaat
     6721 cagccacaaa ttacttgctt ccgggagccg tggcgaagcg atgccgtggg cgatcatcat
     6781 caactgattc tctttaatca tccgccactg ttcatcaagc gatattgccg cccgctcgct
     6841 atgtgcggca gcttgctgcg gttcgtaaat cgtcacccgg ccccggccac catttttgga
     6901 ggcataacag gcgatatcag cctgcgacat tacttcagcc gcctgatgat tgttgtcatc
     6961 aatcaaggta atcccggcac tggcacctac ccgatgcaca cgtccttccc atataaagtg
     7021 atagtcattc acggcactga taatgcgtgt agcgataaaa cgagcgcttt caacattgca
     7081 gtctggcagt agcagaccaa attcatcacc accgagccgc gccagcacgt cgctggagcg
     7141 cagcatactc agcatcaacg acgccagttc gcgcagtaaa gcgtcgccag cggcgtgccc
     7201 ggcgctgtca ttcaccgctt taaagcgatc aagatcgata aacaccaggg catgtcgctg
     7261 atgcgtactg tttaccgttt gcagcaggat acgcagttgc ttctcaaaac tggcgcgatt
     7321 ggcgagatgc gtcagtgcat catgggaggc gctgtagctc agctggcgca gcattttgcg
     7381 tgattcggta acgtcctgaa tcaccagtac cgagcctata ttgctgccgt ccagagtact
     7441 taacggcgta atactgtaat gcacgtcgta gctgccgccg ctccggcagt gcaataccac
     7501 atcttgttca atcgcggaac gtgaggtgtc agcactgtaa atgttctcca ttaatggtcc
     7561 gttgtcgcca aaagtaatat gcaacaccgt caggagcgga acacctaacg cttcttcctg
     7621 cgtccagccg ctcatcttct ccgccactgg attcataaag gtaattttca ttgccatatc
     7681 aatacagacc acggcttcgc cgatggagtc cagcgtaatg tgcaggcgct ctttttcctg
     7741 aaacaatgcc tcgttaagct gtttaacctc cgtcatatcc atattaatgc cgagcaggcg
     7801 ttcaacttcg ccttctttat tcagtacccg gttggcgaga gcgcggatat ggcgaatacc
     7861 gtctttcacg gcaatgcgaa attccagttt aaagggcgag cgtgattgca acgaatcacg
     7921 aatcactttt tcggcgtgct ggcgatcttc cggcagcacg cactcgtacc atacctgcca
     7981 gttcggtttg atatgcggag gaatttcata cagctcgaac atccgcttat cccaactaaa
     8041 aatattcggc ttcagctccc actcccagat gccaatcccg cccgcttcgt tggccagcgt
     8101 gatgcgctcc atcagttgtt gattgacctg ttcggtgcgt tttagctcgt taatgtcttc
     8161 aatctgagcg ataaaataga gcggcgtgcc atccgtgtgg cgcaccagtg agacggcaag
     8221 caacgcccag acaacatcgc cattgcggtt gtagtaacgt ttttccattg aataggtgtt
     8281 tatttcaccg cttatcagct tttcaacctg ttggagatct ttattgagat cctccggcca
     8341 ggtcagttgc tgaaaggtga gtccgcgcag ctcttcctga ctgtaaccga gaaactggca
     8401 gagcgctttg ttggattgca gccattgtcc ctcggtgccc actaacgcca tgccgatagc
     8461 ggaatattcc atcgcgttcc gaaagcgggt ttcgctttcg gaaatgtgtt tgcgttccgc
     8521 acgaaaggca tacatcacca tcgtcatgat gttggcgggc agcaggatca gcaaaaacgg
     8581 tagccacggc atatggctca tcaggtacgt acgcggcgta gcaagcaggg agggatccgc
     8641 ggccatcatc agcgatacca tcatcactgt ggtaaggaag atcaaaaagg cttccattcg
     8701 tggcaggcgc acggcgctcc acatcaacag cacaataatg aaagtaaaag gccacggcag
     8761 atacagcatc gaaagccagc ttaacgtcag cgtgatggct aacgtgagca gcgactcaaa
     8821 aagcaacctt gggttgcgat ggcgcagtag atagtgtggt ttaaataaca atcccagcgg
     8881 caccagtgcc agagcgccga tggattctga cagtacccat atcaaaaatg ccctgagagg
     8941 atcgtctccg ggcgtcagca ggacaaccag aacacccccc aacagaggcg gaataatggc
     9001 gctgccgagt gccagacgca gccagtcagc cagattttgc aaggggttat accacggcag
     9061 caatttacgt agcagcactg ccccgaccac ggcttcaaca atattgatgg tcgtccaggt
     9121 catgttcagc gagctggtgg aaaaaagcag gatggatgcg gcgatatttc ccagcgagca
     9181 gctcagcgca attcccggcc acatgcgccc ggcatggcga taaaacgcca ccatcatgat
     9241 ggacgtcggg aaccacagtg gggcgagttg ggtgccaaac tgcgaaagct caagtgagaa
     9301 aagggtgaag ataaacgaga ctaaacctaa actgaccagg tgaagcagcg ggtggggcag
     9361 ggcaattaat acatgctgtg attgtttgct cattaccgct ttatccgcaa ggccaggtcg
     9421 ccagtattcg tttctgtgtt tatgctagta caaaagtttt acattttata tgcgaattgc
     9481 tcataaagtg acgtaaaggc ggataatttg cgcaactgcg tataacattt tttaccttgt
     9541 ataaaactga tcaacgtaat ttgccaccgg gaattcttcg tcaacaatta actctgagcg
     9601 cgcggcaacg ctattcgact ggtatcaggc ggatgaaatc cctataattg ccgcgtttgg
     9661 cgcttcgtcg cccccttcct aacatccagg ttaatcaggt cgctaaatat atgactgatc
     9721 agtctcatca gtgcgtcatt atcggtatcg ctggcgcatc ggcttccggc aagagtctta
     9781 ttgccagtac cctttatcgt gaattacgtg agcaagtcgg tgatgaacac atcggcgtaa
     9841 ttcccgaaga ctgctattac aaagatcaaa gccatctgtc gatggaagaa cgcgttaaga
     9901 ccaactacga ccatcccagc gcgatggatc acagcctgct gcttgagcat ttacaagcgt
     9961 tgaaacgcgg ctcggcaatt gacctgccgg tttacagcta tgttgaacat acgcgtatga
    10021 aagaaacggt gacggttgag ccgaagaagg tcatcattct cgaaggcatt ttgttgctga
    10081 cggatgcgcg tttacgtgac gaacttaact tctccatttt cgttgatacc ccgctggata
    10141 tctgcctgat gcgccgcatc aagcgtgacg ttaacgagcg tgggcgttca atggattcag
    10201 tgatggcgca atatcaaaaa accgtgcgtc cgatgttcct gcaattcatt gagccttcta
    10261 aacaatatgc ggacattatc gtgccgcgcg gcgggaaaaa ccgcatcgcg atcgatatat
    10321 tgaaagcgaa aataagtcag ttctttgaat aagcttgata aattgtgtac cgttcagtga
    10381 taacctggta tgcccttgac gtaattggcg ttaagggagt gatgctcgaa aggagaaaat
    10441 gccatgcgtc tgtgtgaccg agatattgaa gcctggcttg atgaaggccg tttgtcgatc
    10501 aacccacgtc cgccagtgga gcgtattaac ggcgcgacgg tggatgtgcg cctgggcaat
    10561 aaatttcgta ccttccgtgg tcacacggca gcgtttatcg atctgagcgg tcctaaagat
    10621 gaagtgagcg ccgcgcttga ccgcgtgatg agcgatgaaa tcgtgctcga cgagggcgag
    10681 gcgttttatc ttcacccagg agagctggcg ctggcggtga cgctggagtc ggtgacgctg
    10741 ccagccgatc tggtgggctg gctggacggg cgttcttcac tggcgcgtct ggggctgatg
    10801 gtgcacgtca ccgcgcaccg tatcgatccg ggctggtctg gctgcattgt gctggagttt
    10861 tacaactccg gtaagctgcc gctggcgctg cgtccgggca tgttaattgg tgcgctgagc
    10921 tttgagccgc tctccggccc ggcggcgcga ccttacaacc gccgtgaaga tgcgaaatat
    10981 cgcaaccagc agggcgcggt agccagccga atcgataaag actaattcag gcccattgag
    11041 gataccatga gacgatttct gacgacgctg atgatactcc tggtcgtgct ggtggccggg
    11101 ttatctgcgt tagtgttgct ggtgaatccg aatgatttcc gcgactatat ggtcaagcaa
    11161 gttgctgcac gtagcggtta tcaattgcag ctcgacgggc cactgcgttg gcacgtctgg
    11221 ccgcagctta gtatcctctc cgggcgaatg tctctcaccg cccagggcgc aagccagcca
    11281 ctggttcgcg ccgacaacat gcgtctggac gtggcgcttt taccactact gagtcatcaa
    11341 ctgagcgtta agcaggtgat gctaaaaggg gcagtgatcc aactgacgcc gcagacggaa
    11401 gcggtgcgca gtgaagacgc tccggttgca ccgcgcgaca ataccttgcc ggatctgtca
    11461 gacgatcgcg gatggtcgtt tgatatatcc agtcttaagg tggcggacag cgttctggtg
    11521 ttccagcatg aagatgacga gcaggtgaca atccgcaata tccgcctgca aatggaacaa
    11581 gatccccaac atcgtggctc atttgagttt tccgggcggg ttaatcgcga tcagcgcgat
    11641 ctcacattat cccttaacgg tacggtggat gcttctgatt atccgcatga tttaacggcg
    11701 gctattgaac aaattaactg gcagttgcag ggtgccgatt taccaaaaca aggtattcag
    11761 gggcagggga gtttccaggt ccagtggcag gagtcacata aacgcctttc atttaaccaa
    11821 attagtttga cctccaatga cagtacgctg agcgggcaag cacaggtcac gttgacagag
    11881 aaaccggaat ggcagctgag actgcaattc ccgcaactga atcttgacaa cctcatcccc
    11941 cttaatgaaa cagcgaatgg tgagaacggt gccgcgcagc aagggcagag ccaatcaacg
    12001 ttgccgcgcc cggttatttc ttcgcgtatt gatgaaccgg cctatcaggg actgcaaggc
    12061 tttacggctg atattttgtt gcaggccagt aacgtgcgct ggcgcggaat gaattttaca
    12121 gatgttgcca cgcaaatgac caacaagtcg ggtttgctgg aaattactca actgcagggc
    12181 aaacttaacg gtggacaggt ttcactgccg ggcacgttgg acgcgacatc aataaatccg
    12241 cggataaact tccagccacg gctggaaaac gttgagattg gcaccattct gaaggcgttt
    12301 aactatccga tttcgttgac cggaaagatg tcactggctg gtgatttctc cggtgctgac
    12361 atagatgccg acgcattccg ccacaactgg caaggacagg cacatgtcga aatgaccgac
    12421 acacgcatgg aagggatgaa cttccagcag atgattcagc aagcggtaga acgtaatggt
    12481 ggtgatgtga aggccgctga aaacttcgat aacgtgacgc gtcttgaccg ctttaccacc
    12541 gatttgacgt tgaaggatgg cgtcgtgacg ttaaacgaca tgcaaggtca atcgccagtg
    12601 ctggcgctga caggggaagg catgttgaat ctggcagatc aaacctgcga cacccagttt
    12661 gatattcggg tcgtgggtgg ctggaacgga gaaagcaaac tgattgattt cctcaaagaa
    12721 acgccagtac cgctgcgggt ttatggcaac tggcagcaac ttaattacag cctgcaagtg
    12781 gatcagttac tgcgtaaaca tctacaggat gaagcaaaac gccgtcttaa cgactgggcc
    12841 gagcggaata aagattcccg caatggcaaa gatgtgaaga agttgctgga gaagatgtag
    12901 gttaccaggt gagctgtcct gtaggcctga taagacgcgt aaagcgtcgc atcaggccta
    12961 catgccatca cacctcgtac tccatctcgc catccttatg cagcggaata atctgcacct
    13021 tctgcacgcg atggctttcc acctgcaacg ttttaagcaa ataatccccc acctgaactt
    13081 cttcgccagg ttttggaata cgctgcaaat attccatcaa tagcccggca atggtgtgat
    13141 attcacgttt ttcatccagc ggcagcggca catattgcac cagatcttcc agcggcatat
    13201 gaccattcgc cgtccaggaa ccgtccgcat tcttctgaat atcatggcgg gcgtcgatct
    13261 cttccacttc attcggtaag ttaccggcaa tggtttcagt gacgtcactt aatgtcacaa
    13321 tcccttccac cgagccaaac tcatccacca caaaagcaaa gtgtgtgcgg gcattacgga
    13381 actgctccag ggcaggtaac aacggcaagg tttccgggaa caccagcggc tgacgaatca
    13441 acacccgcag gttgagcggt tcgccgcgca gtgactgttg taacaggtcg ataacgtgaa
    13501 caacaccgag caaatcttct gcgtcatcgc catcagtaac aacaagccgc gtatgctgat
    13561 ttcgctccag taactggcgg atctcgtctt ccggcgcgtt gagatcgata tgctcaatat
    13621 cgtggcgcga cgtcataatg ctactgacgg tacgctggtt aagattaagt acccgctcaa
    13681 tcatccgccg ttcctgcgga ttaaagatct gctggttacc atgatccacc agcatggacg
    13741 cggtttcggc gtccagttcc gcatcttctt tttgcccgct aagcaggcgc attaccgctt
    13801 cggtagtccg ctggcgcagt gtctggttag cggaaagaaa acgtcgccgg ttaaagatag
    13861 ccaactgatt gagcgcctcg atcatcaccg agaaaccaat ggcagcgtac aggtagcctt
    13921 tcggaatgac gaagccgaaa ccttccgcca ccaggctaaa gccaatcatc aacaggaagc
    13981 ttaagcagag aataacgatg gtcgggtgac tgttaacgaa ctgggttaac ggcttgctgg
    14041 ccatcaacat caggctgata gcgataacca cggcggccat catgaccagt aaatggtcga
    14101 ccatcccgac ggcggtaatc accgagtcga gtgagaagat ggcgtccagt accacaattt
    14161 gtgtcaccac cccccagaac ttcgcacctt tgcgttgggt ggggttattg ctgtctttcc
    14221 cttccagccg ttcgttcagc tccatcgtgg ctttgaacaa caggaagaaa ccaccaaaca
    14281 gcatgattaa gtcgcgggcg ctaaaggtaa acgagcggaa gctgaacagc ggttgagtca
    14341 gggtgaccag ccaggagatt gacgccagca gtaacaggcg cattaacatc gccagcagga
    14401 gcccggtaac ccgtgcgcgg tcgcgctgct taggcggtag tttttcggcg aggatggcaa
    14461 taaagaccag gttatcaatg ccgaggacca gttcgatcac aatcagcgtg attaacccag
    14521 cccagataga cggatcggca atccattcca tagtaagtac aaaacctatt cgttgtatga
    14581 caaatgtcac aattcgatca tggataaatc ggggcaaaaa tgcaatgttg cagcactatt
    14641 tatctcatat tatttgaggg ttataacctc acattatccc gaattataag tggtaataat
    14701 aaagcgttat ttaagaaata tcgcatgtgg agacgccata ttaaatattg tcaacctaaa
    14761 gaaactccta aaaaccatat tgaatgacac ttaatataat tcttaaaaat agccaattgc
    14821 cgaattgtta ccttgcctgc tattccgtta gctgtaacac ttcctcctgc attattggag
    14881 agccaatatt caattaccat aatgtcacct tatattcgga aattaatttc tgcaataacc
    14941 ctttctcatc aacgactgta tgcagagagg gtttgaaaaa ttaaactaaa tcagtgtatt
    15001 ggtagctaaa aagccagggg cggtagcgtg tctggatgcc tgagagacca gtctgaatta
    15061 ttctgtcaat agcctgcgga tgaaatcaat ttttttagga ctgatgccag ttaaattttt
    15121 attcaattaa ctgcaaggta aatgtgcatg gcgacattgc tattaatagt gcacaggata
    15181 attactctgc caaagtgata aataaacaat gatgaaatcc aaaatgaaat tgatgccatt
    15241 attggtgtca gtaaccttga taagcggttg cacagtactt ccgggcagca atatgtcgac
    15301 gatggggaaa gacgtcatca aacagcagga cgctgatttc gacctcgaca aaatggtgaa
    15361 tgtttatccg ttaaccccgc gcctgattga tcaattacgc ccacgaccca acgtagcgcg
    15421 ccccaatatg acgctggaaa gtgagatcgc gaattaccag tatcgcgtcg ggccggggga
    15481 cgttcttaat gtcaccgtct gggatcaccc ggaactcact acaccagccg gtcagtaccg
    15541 cagctccagc gacaccggca actgggtaca gcctgacggc accatgttct acccgtatat
    15601 cggcaaggtt cacgtcgtgg ggaaaacgct cgctgaaatc cgcagtgata ttaccgggcg
    15661 cttagcgacg tatatcgctg acccgcaggt ggacgttaat atcgccgcct tccgctcgca
    15721 aaaagcctat atctccggtc aggtgaataa atccggtcaa caggcgatta ccaacgtgcc
    15781 actgaccatt ctcgacgcca tcaacgccgc aggtggcctg accgacaccg ctgactggcg
    15841 caacgtggtg ctaacacaca atggtcgtga agagcgcatt tctttgcagg cgctgatgca
    15901 aaacggcgac ctcaatcaga accgactgct ttaccccggc gatattcttt acgtgccacg
    15961 taatgatgat ctgaaagtgt ttgtgatggg tgaagtgaag aaacagagca ccctgaaaat
    16021 ggactttagc ggcatgaccc tgactgaagc cctgggcaat gctgaaggca tcgacatgac
    16081 cacctccaac gccagcggca tctttgtcat tcgtccgctg aaaggcgaga gcgggcgtaa
    16141 cggcaagatt gccaatatct accagctgga tatgtccgat gcgacgtcgc tggtaatggc
    16201 gacagaattc cgcctgcaac cttatgacgt ggtgtatgtc accaccgccc cggtatcccg
    16261 ctggaaccgt ctgatcaatc agttgctgcc aactattagc ggtgtccgtt acatgacgga
    16321 tacagccagc gacattcata actggtaatc gtcatgttta acaacatctt agttgtctgt
    16381 gtcggcaata tttgccgttc cccgacggcg gaacgcttac tgcaacgtta tcacccggag
    16441 ctaaaagtgg agtccgctgg actcggcgcg ctggtcggta agggcgctga tcctaccgct
    16501 atcagcgtcg ccgcagaaca tcaactgtcc ctggaaggtc attgcgcacg gcaaatcagc
    16561 cgccgtctgt gtcgcaacta cgacctgatt ctgaccatgg aaaagcgcca tatcgaacgc
    16621 ttatgcgaga tggcaccaga gatgcgcggc aaagtgatgc tgtttggtca ctgggataac
    16681 gaatgtgaaa tccccgatcc gtatcgcaaa agccgggaaa cgtttacagc ggtgtacaca
    16741 ttacttgaac ggtctgcccg ccagtgggcg caggcattga acgcagagca ggtataagaa
    16801 tgacagaaaa agtaaaacaa catgccgctc cggtaacggg cagtgatgaa atcgatattg
    16861 gtcgcctggt cggcaccatc attgaagcac gctggtgggt gattggcatc accgccgtat
    16921 tcgccctctg tgccgtggtt tacaccttct tcgctacgcc gatttatagc gccgacgcac
    16981 tggtacaaat cgagcaaagc agcggcaatt cgttagtgca ggatatcgga tcggcattag
    17041 ccaacaaacc gcctgcatcg gacgccgaaa tccagttgat tcgttcgcgc ctggtgcttg
    17101 gtaaaacggt ggacgatctc gacctcgata ttgcagtgag caaaaacacg ttcccgattt
    17161 tcggtgcggg ctgggagcgc ctgatgggac gccagaacga gacggtaaaa gtgaccacct
    17221 ttaaccgccc gaaagagatg gcggatctgg tgtttacgct taatgtgctg gacgataaaa
    17281 actacactct gagcagcgac ggcggcttta gcgcccgtgg gcaagcgggc cagatgctga
    17341 aaaaagaagg cgtcacactg atggttgaag ccattcacgc cagcccgggc agtgagttta
    17401 ccgtcaccaa atactccacg ctggggatga tcaaccaact gcaaaacagc ctgacggtaa
    17461 cggagaacgg caaagacgca ggcgttttga gcctgactta taccggtgaa gatcgcgaac
    17521 agatccgcga cattcttaac agcatcgccc gtaactatca ggaacaaaat attgagcgca
    17581 aatcggcgga agcgtcgaaa agcctcgctt tcctcgcgca acagttaccg gaagtacgta
    17641 accgccttga tgttgccgaa aacaaactga atgccttccg tcaggataaa gattcagttg
    17701 atctgccgct ggaagcgaaa gcggtgctcg attcgatggt gaacatcgac gcgcagttga
    17761 acgaactgac ctttaaagag gcggaaatct ccaagctgta caccaaagtt cacccggcgt
    17821 accgcacgct gctggagaaa cggcaggcgc tggaagacga aaaagccaaa cttaatggtc
    17881 gcgtaacggc gatgccgaaa acccagcagg aaattgtgcg tctgacccgc gatgtcgagt
    17941 ctggtcagca ggtctatatg caactgctga ataaagagca ggagctgaaa atcaccgaag
    18001 ccagcaccgt cggcgatgtg cgcattgttg acccggcaat cacccagcca ggtgtactga
    18061 aaccgaagaa agggctgatt atccttgggg cgattatcct tggcctgatg ctctctatcg
    18121 tgggtgtgct gctgcgctcg ttgtttaatc gcggtatcga aagcccgcag gtgctggaag
    18181 aacacggtat cagcgtttat gccagcatcc cgctgtcgga gtggcagaaa gcgcgcgata
    18241 gcgtcaaaac catcaaaggg attaaacgct ataaacagag ccagctactg gcggtgggga
    18301 atccaaccga tctggcgatt gaagccatcc gtagtctgcg taccagtctg cacttcgcga
    18361 tgatgcaggc acagaacaat gtgttgatga tgaccggggt tagcccgtca atcggtaaaa
    18421 cctttgtctg cgccaacctg gcggcggtga ttagccagac caataaacgg gtgttgttga
    18481 tcgactgcga tatgcgcaaa ggctacaccc acgagctgtt gggcactaat aacgttaatg
    18541 gcctgtcgga aattctgatt ggacagggcg atattactac cgctgctaaa ccgacctcta
    18601 ttgccaaatt tgacctgatc ccgcgcggtc aggtaccgcc aaatccttct gaactgttaa
    18661 tgagcgaacg ctttgccgaa ctggtgaagt gggcgagtaa aaactacgac ctggtgttga
    18721 ttgatacgcc gccgattctg gcagtgaccg atgcggcaat tgttggtcgt catgtcggaa
    18781 ccacgttgat ggtggcgcgt tatgcagtca acacattgaa agaagtggaa accagtctga
    18841 gccgctttga gcaaaacggt attccggtga aaggggtgat tctgaactcc atcttccgcc
    18901 gcgccagcgc atatcaggat tatggctatt acgaatacga atataagtcg gatgcgaaat
    18961 aacgacgcct gcattcgcac cgccccgtag gccggataag gcgttcacgc cgcatccggc
    19021 aagcaaacca gctcataagc cgggagaaca acctatgaaa gacaatccgc tgatctcaat
    19081 ctatatgccg acctggaatc gccaacaact ggcgattcgg gcgataaaat cggtcctgcg
    19141 ccaggactat aacaactggg agatgatcat cgtggatgat tgctccacct cctgggaaca
    19201 actccaacag tacgtcaccg ccctcaacga tccgcgtatc acttacattc acaacgacat
    19261 taacagcggg gcgtgcgcgg tacgtaacca ggcgattatg ctggcgcaag gggaatacat
    19321 caccgggatc gatgacgacg atgaatggat acccaaccgc ctgagcgtct tcctcgccca
    19381 taaacagcaa ctggtcacac acgccttttt gtacgctaac gactacgttt gccagggcga
    19441 agtttattcc caaccggcaa gcttgccgct gtatcccaaa tcgccgtatt cacgccgcct
    19501 gttctacaaa cgcaatatca ttggcaatca ggtctttacc tgggcgtggc gtttcaaaga
    19561 gtgcttgttc gataccgaac tgaaagcggc acaggattac gacatcttcc tgcggatggt
    19621 ggtggagtac ggcgaaccgt ggaaagtgga agaggcgacg cagatcctgc atatcaatca
    19681 cggcgaaatg cagatcacct cgtcaccgaa aaaattctcc ggatacttcc acttttaccg
    19741 caagcacaaa gacaaattcg accgcgccag caaaaaatat cagctgttta ccctctacca
    19801 gatccgcaat aaacgcatga cctggcgcac tttgctgacg ctgctgtcgg tgcgtaacgg
    19861 caaacgtctg gctgatggta ttcgggggcg ctaaagatgc tggaagatct gcgcgccaac
    19921 agctggagtt tacgcccgtg ctgcatggtt ctcgcctatc gcgtcgctca tttttgttcg
    19981 gtgtggcgca aaaagaacgt cctcaacaat ctgtgggcgg ccccgctgct ggtgctgtat
    20041 cgcattatca ccgaatgttt tttcggttat gaaatccagg ccgccgcgac cattggccgc
    20101 cgctttacta tccatcacgg ttacgccgta gtgatcaata aaaacgtggt agcgggggat
    20161 gatttcacca ttcgccacgg cgtcactatc ggcaatcgtg gtgccgataa catggcatgt
    20221 ccacacattg gcaacggcgt cgaactcggt gccaacgtca ttattcttgg tgatatcacg
    20281 cttggtaaca acgtcaccgt gggcgcgggc agcgtggtgc tcgactctgt cccggacaac
    20341 gcgctggtgg tgggagaaaa agcgcgagtg aaggtaatta aatgaatatt ctgcaattta
    20401 atgtgcgact ggcggaaggc ggggccgcag gtgtggcgtt agatctccac cagcgtgcgc
    20461 tgcaacaggg gctggcgtca cattttgtct acggctacgg caaaggcggc aaagagagcg
    20521 tcagccatca aaactatccg caggtcatca aacatacgcc gcggatgacc gcgatggcga
    20581 acattgcgtt gtttcgtctg cttaatcgcg atctgttcgg caatttcaat gagttatatc
    20641 gcaccattac tcgcacaccg ggcccggtgg ttctgcattt tcatgtgctg cacagctact
    20701 ggttgaatct taagagcgtg gtgcgctttt gtgaaaaagt gaaaaaccat aaaccggatg
    20761 tcactctggt ctggacactg cacgaccact ggagcgttac cggacgctgc gcctttaccg
    20821 acggttgcga aggctggaaa acgggctgcc agaaatgccc gaccttaaat aattatccgc
    20881 cggtgaagat tgatcgcgca caccaactgg tggcgggcaa acggcagtta tttcgcgaga
    20941 tgctggcgct gggctgtcag tttatttctc ccagccagca tgtggctgat gctttcaata
    21001 gtctgtacgg tccagggcgt tgccggatta tcaataacgg tattgatatg gcaaccgaag
    21061 cgattctggc ggatctgcct ccggtgcgcg aaacccaggg caagccgaaa atcgcagtgg
    21121 tggcgcatga tctgcgttac gacggcaaaa ctaaccagca actggtgcgc gagatgatgg
    21181 cgctgagcga caaaatcgaa ctgcatactt tcggtaagtt ctcgccgttc accgctggta
    21241 acgttgttaa tcacggcttt gaaactgaca agcgcaagtt aatgagcgtg ctcaatcaga
    21301 tggatgcgct ggtgttcagt tctcgcgtcg ataactaccc gctgattttg tgtgaggcgt
    21361 tatcgattgg cgtgccggtg atcgccaccc atagcgatgc ggcgcgggaa gtgctgcaaa
    21421 aatccggtgg taaaaccgtc agcgaagaag atgtgctgca actggtgcag ttaagcaaac
    21481 cggaaatcgc acaggcgata tttggtacca cgctggctga attcagccag cgcagccgcg
    21541 ccgcctacag tggacaacag atgctggagg agtatgtcaa cttctatcag aatctgtagc
    21601 tacctgctgc tgccgctgat ctacctgctg gttaacgtca aaatcgccca gcttggcgaa
    21661 agtttcccca tcaccatcgt cactttttta cctgtcttgt tactgctgtt tttagagcgc
    21721 atcagcgtta aaaaattgat gattgcctta gggattggcg cggggctcac ggcgtttaac
    21781 tacctgtttg gtcagtcgtt ggatgccagt aaatatgtca cttcaaccat gctgtttgtc
    21841 tatattgtga tcattattgg catggtgtgg agtattcgct ttaaaacaat ttcgccacac
    21901 aaccatcgca agatattacg tttcttttat ctggtggtcg ggttggtggt ggtgctggcg
    21961 gcggtggaga tggcgcaaat tatcctcaca ggcggtagca gtattatgga gtcgatttcg
    22021 aaatatctga tttacagcaa cagctatgtg ctgaatttca ttaaattcgg cggcaagcgc
    22081 acgacagcac tttatttcga accggcattt ttcgctctgg cattaatctc aatttggctc
    22141 agcatcaaac agtttggtat caaaacgcct aaaaccgatg ctatgattct cgcagggata
    22201 atattatccg gatcgttttc aggggttatg acctttatcc ttttttattt gctggagtgg
    22261 gcatttcaat atctgaataa agaggcgatt aagaaaaagt taccgttagc attgatttct
    22321 ctggctgtat tcctggttgg tgtggtaatc gcgttccctt atatttccac ccgtctgggc
    22381 gatttaggta cagaaggatc gtcatcgtat tatcgtattg tcggtccgct ggtgatggtc
    22441 ggttattctt tgacccatat tgacggtgta gtcagatttg gctcacttta tgaatatgtc
    22501 gcatcattcg gaatatttaa cggtgcggat gtcggaaaaa ccatagacaa tggtttgtat
    22561 ctgctgatta tttatttttc ctggtgcgca gtgtttttat cgctgtggta catgggaaaa
    22621 gtgataaaaa tgatgatcaa cgcttttggt gataaccgca attttcgcgt gcaattatat
    22681 ctttttactc cggtatcgct gttttttacc ggttcgatat ttagcccgga atatgcattt
    22741 ttaatcgtct gtccgtttat tttgcgaaaa gcgttaaata ttacgaggta agaataagaa
    22801 catgttgctt agcataatca ctgtcgcgtt tcgtaacctc gaaggaatag tcaaaacaca
    22861 cgcctcgctg gcgcatctgg cgcaggcgga cgatatcagc ttcgaatgga ttgttgttga
    22921 tggcggttcc aatgacggca cccgtgagta tctggaaaat ctcaatggta tctataacct
    22981 acgctttgtc agcgagccag ataacggtat ctacgacgcc atgaacaaag gtattgaaat
    23041 ggcgcaaggc aagttcgcgc tgtttctcaa ttcaggcgat atttttcatc aggatgccgc
    23101 aaattttgtc cgtaagttaa aagtgcaaaa agataacatg atgatcaccg gcgatgcgct
    23161 gctggatttt ggcgacgggc ataaaattaa acgtagcgcc aaaccgggtt ggtatattta
    23221 tcacagcctg cccgccagtc atcaggcgat atttttcccg gtatccggtt tgaaaaaatg
    23281 gcgttatgac ctggaatata aagtttcttc cgattacgcg ctggcagcca aaatgtataa
    23341 agcaggttat gcatttaaaa aactcaatgg cctggtgtct gaattttcta tgggtggggt
    23401 atcaaccacc aataatatgg aattgtgtgc tgacgccaaa aaagtccaac ggcagatatt
    23461 gcacgtgcct ggcttttggg ctgaattatc ctggcattta cgccaacgta cgacctcaaa
    23521 gacgaaagcc ttatataaca aaagctgaat ataagggaaa acgatgcagg atttaagtgg
    23581 attctcggtg ccgaaagggt tccggggtgg caacgctatt aaagtgcaat tgtggtgggc
    23641 agtacaggca acaatatttg cctggtcgcc acaagtattg tatcgctggc gggcattttt
    23701 attacgttta ttcggcgcaa aaataggaaa aaacgtagtt attcgtccgt cagtaaaaat
    23761 tacctatccg tggaaattaa ccttaggtga ttacgcgtgg gtcggcgatg aggtcaattt
    23821 atataccctc ggtgaaataa ccattggcgc acattcggtg atatcgcaaa aaagttattt
    23881 atgcaccggg agtcacgacc atgcaagtca acatttcacc attaacgcca cgcctattgt
    23941 gattggcgag aaatgctggc tggcaaccga tgtctttgtt gcccctggcg tcacgatcgg
    24001 cgacggcacc gtcgtgggtg cgcgaagcag tgtttttaaa tcgcttccgg caaatgtggt
    24061 ttgccggggg aaccccgcag tggtgatacg caaacgcgtt gaaactgaat aaattcaaaa
    24121 aatacagagg aataagacat gtcaaaagtc gctctcatca ccggtgtaac cggacaagac
    24181 ggttcttacc tggcagagtt tctgctggaa aaaggttacg aggtgcatgg tattaagcgt
    24241 cgtgcatcgt cattcaacac cgagcgcgtg gatcacattt atcaggatcc gcacacctgc
    24301 aacccgaaat tccatctgca ttatggcgac ctgagtgata cctccaacct gacgcgtatt
    24361 ttgcgtgaag tgcagccgga tgaagtgtac aacctgggcg caatgagcca cgttgcggtt
    24421 tcttttgagt caccggaata taccgcagac gtcgatgcga tgggtacgct gcgcctgctc
    24481 gaggcgatcc gcttcctcgg tctggaaaag aaaacccgtt tctatcaggc ttctacttct
    24541 gaactgtacg gtctggtgca ggaaattccg cagaaagaga ccacgccgtt ctacccgcga
    24601 tctccgtatg cggttgccaa actgtacgct tactggatca ccgttaacta ccgcgaatcc
    24661 tacggcatgt acgcctgtaa cggtattctc ttcaaccatg aatccccgcg ccgtggcgag
    24721 actttcgtta cccgcaaaat cacccgcgca atcgccaaca tcgcccaggg gctggagtcg
    24781 tgcctgtatc tcggcaatat ggattccctg cgtgactggg gccatgccaa agactacgta
    24841 aaaatgcagt ggatgatgct gcaacaggaa caaccggaag atttcgttat tgccaccggc
    24901 gttcagtact ccgtgcgtca gttcgtggaa atggcggcgg cacagctggg tatcaaactg
    24961 cgctttgaag gcacgggtgt tgaagagaag ggcattgtgg tttccgtcac cgggcatgac
    25021 gcgccgggcg ttaaaccggg tgatgtgatt atcgccgttg acccgcgcta cttccgtccg
    25081 gctgaagttg aaacgctgct cggtgacccg accaaagcgc acgaaaaact gggctggaaa
    25141 ccggaaatca ccctcagaga gatggtgtct gaaatggtgg ctaatgacct tgaagcggcg
    25201 aaaaaacact ctctgctgaa atctcacggc tacgacgtgg cgatcgcgct ggagtcataa
    25261 gcatgagtaa acaacgcatt tttatcgctg gccatcgcgg gatggtcggt tccgccatca
    25321 ggcggcagct tgaacagcgc ggtgatgtgg aactggtatt acgcacccgc gacgagctga
    25381 acctgctgga cagccgcgcg gtgcatgatt tctttgccag cgaacgtatt gaccaggtct
    25441 atctggcggc ggcgaaagtg ggcggcattg ttgccaacaa cacgtatccg gcagatttca
    25501 tctaccagaa tatgatgatt gagagcaaca tcattcacgc cgcgcatcag aacgacgtga
    25561 acaaactgct gtttctcgga tcgtcctgca tctacccgaa actggcaaaa cagccgatgg
    25621 cagaaagcga gttgttgcag ggcacgctgg agccgaccaa cgagccttac gccattgcca
    25681 aaatcgccgg gatcaaactg tgtgaatctt acaaccgcca gtacggacgg gattaccgct
    25741 cagtcatgcc aaccaacctg tacgggccgc acgacaactt ccacccgagt aactcgcatg
    25801 tgatccctgc attgctgcgt cgcttccacg aggcgacggc acagaatgcg ccggacgtgg
    25861 tggtgtgggg cagcggtacg ccgatgcgcg aatttctgca cgttgatgat atggcagcgg
    25921 cgagcattca tgtgatggag ctggcgcacg aagtctggca ggagaatacc cggccgatgc
    25981 tgtcgcacat taacgtcggc acgggcgttg actgcactat ccgcgagctg gcgcaaacca
    26041 tcgccaaagt ggtgggttac aaaggtcggg tagtttttga tgccagcaaa ccggatggca
    26101 cgccgcgcaa attgctggat gtgacgcggc tgcatcagct tggctggtat cacgaaatct
    26161 cactggaagc ggggcttgcc agcacttacc agtggttcct tgagaatcaa gaccgctttc
    26221 gggggtaatg atgtttttac gtcaggaaga ctttgccacg gtagtgcgct ccacaccgct
    26281 tgtctctctc gactttattg ttgagaacag tcgcggcgag tttctgcttg gcaaaagaac
    26341 caaccgcccg gcgcagggtt actggtttgt gccgggaggg cgcgtgcaga aagacgaaat
    26401 gctggaggcc gcatttgagc ggctgacgat ggcggaactg gggctacgtc tgccgataac
    26461 agcaggccag ttttacggtg tctggcagca cttttatggc gataacttct ccggcacgga
    26521 attcaccact cactatgtgg tgctcggttt tcgcatcaga gtcgcggaag atgagctgtt
    26581 actgccggat gagcagcatg acgattaccg ctggctgacg ccggacgcgt tgctcgccag
    26641 tgacaatgtt cacgctaaca gccgcgccta ttttctctct gagaagcgtg ccggagtacc
    26701 cggattatga aaatactggt ctacggcatt aactactcgc cggagttaac cggcatcggc
    26761 aagtacaccg gcgagatggt ggaatggctg gcagcacaag gtcatgaggt gcgggttatt
    26821 accgcgccgc cttactaccc gcagtggcag gtgggcgaga actattccgc ctggcggtac
    26881 aaacgggaag agggggccgc cacggtgtgg cgctgcccgc tgtacgtgcc aaaacagccg
    26941 agcaccctga aacgcctgtt gcatctcggc agttttgccg ccagcagttt ctttccgctg
    27001 atggcgcaac gtcgctggaa gccggatcgc attatcggcg tggtgccaac gctgttttgc
    27061 acgccaggaa tgcgcctgct ggcgaaactg tctggtgcgc gcacggtgct ccatattcag
    27121 gattacgaag tggacgccat gctggggctg ggccttgccg gaaaaggtaa aggcggcaaa
    27181 gtggcacagc tggcaacggc gttcgaacgt agcggactgc ataacgtcga taacgtctcc
    27241 acgatttcgc gttcgatgat gaatagagct atcgagaaag gcgtggcggc ggaaaacgtc
    27301 atcttcttcc ccaactggtc agaaattgcc cgttttcagc atgtcgcaga cgccgatgtt
    27361 gatgcccttc gtaaccagct tggcctgccg gataacaaaa aaatcattct ttactccggc
    27421 aatattggtg aaaagcaggg gctggaaaac gttattgaag ctgccgatcg cctgcgcgat
    27481 gaaccgctga tttttgccat tgtcgggcag ggcggcggca aagcgcggct ggaaaaaatg
    27541 gcgcagcagc gtggactgcg caacatgcaa ttttttccgc tgcaatcgta tgacgcttta
    27601 cccgcactgc tgaagatggg ggattgccat ctggtggtgc aaaaacgcgg cgcggcagat
    27661 gccgtattgc cgtcgaaact gaccaatatt ctggcggtag gcggtaacgc ggtgattacc
    27721 gcagaagccc acaccgaact ggggcagctt tgcgaaacct ttccgggcat tgcggtttgc
    27781 gtggaaccgg aatcggtcga ggcgctggtg gcagggattc gtcaggcgct cctgctgccc
    27841 aaacacaaca cggtggcacg tgaatatgcc gaacgcacgc tcgataaaga gaacgtgtta
    27901 cgtcaattta taaatgatat tcggggataa ttatggcgca gtcgaaactc tatccagttg
    27961 tgatggcagg tggctccggt agccgcttat ggccgctttc ccgcgtactt taccccaagc
    28021 agtttttatg cctgaaaggc gatctcacca tgctgcaaac caccatctgc cgcctgaatg
    28081 gcgtggagtg cgaaagcccg gtggtgattt gcaatgagca gcaccgcttt attgtcgcgg
    28141 aacagctgcg tcaactaaat aaacttaccg agaacattat tctcgaaccg gcggggcgta
    28201 acaccgcacc tgccattgcg ctggcggcgc tggcggcaaa acgtcatagc ccggagaacg
    28261 acccattaat gctggtgctg gcggcggatc atgtgattgc cgacgaagat gcgttccgcg
    28321 ccgccgtgcg taacgccatg ccgtatgctg aagcgggcaa gctggtgacc ttcggcattg
    28381 tgccggatct accagaaacc ggttatggct atattcgtcg cggtgaagtg tcggcgggtg
    28441 agcaggatac ggtggccttt gaagtggcgc agtttgtcga aaaaccgaat ctggaaaccg
    28501 cgcaggccta tgtggcaagt ggcgaatatt actggaacag cggtatgttc ctgttccgtg
    28561 ccggacgtta tctcgaagaa ctgaaaaagt atcgtccgga tattctcgat gcctgtgaaa
    28621 aagcgatgag cgccgtcgat ccggatctcg attttattcg tgtggatgaa gaggcgtttc
    28681 tcgcctgtcc ggaagagtcg gtagattacg cggtcatgga acgcacggca gatgccgttg
    28741 tggtgccgat ggatgcgggc tggagcgatg tcggttcctg gtcctcttta tgggagatca
    28801 gtgcccacac cgccgagggc aacgtttgcc atggcgatgt gattaatcac aaaactgaaa
    28861 acagctatgt gtatgccgaa tctggcctgg tcaccaccgt cggggtgaaa gatttggtgg
    28921 tagtgcagac caaagatgca gtgctgattg ccgaccgtaa cgcggtgcag gatgtgaaaa
    28981 aagtggtcga gcagatcaaa gctgatggtc gccatgagca tcgggtgcat cgcgaagtgt
    29041 atcgtccgtg gggcaaatat gactctatcg acgcgggcga ccgctaccag gtgaaacgca
    29101 tcaccgtgaa accaggcgaa ggcttgtcgg tacagatgca ccatcaccgc gcggaacact
    29161 gggtggttgt cgcgggaacg gcaaaagtca ctatcgacgg tgatatcaaa ctgcttggtg
    29221 aaaacgagtc catttatatt ccgctggggg cgacgcattg cctggaaaac ccggggaaaa
    29281 ttccgctcga tttaattgaa gtgcgctccg gctcttatct cgaagaggat gatgtggtgc
    29341 gcttcgcgga tcgctacgga cgggtgtaaa cgtcgcatca ggcaatgaat gcgaaaccgc
    29401 gatgtaaata acgacaaaaa caaaattggc cgtttcggtc agggccaact attgcctgaa
    29461 aaagggtaac gatatgaaaa aattaacctg ctttaaagcc tatgatattc gcgggaaatt
    29521 aggcgaagaa ctgaatgaag atattgcctg gcgcattggt cgcgcctatg gcgaatttct
    29581 caaaccgaaa accattgtgt taggcggtga tgtccgcctc accagcgaaa ccttaaaact
    29641 ggcgctggcg aaaggtttac aggatgcggg cgtcgacgtg ctggatatcg gcatgtccgg
    29701 caccgaagaa atctatttcg ccacgttcca tctcggcgtg gatggcggca ttgaagttac
    29761 cgccagccat aacccgatgg attacaacgg catgaaactg gtgcgcgaag gggctcgccc
    29821 gatcagcggc gataccggac tgcgcgacgt ccagcgtctg gcagaagcca acgactttcc
    29881 tcccgtcgat gaaaccaaac gcggtcgcta tcagcaaatc aatctgcgtg acgcttacgt
    29941 tgatcacctg ttcggttata tcaatttcaa aaacctcacg ccgctcaagc tggtgattaa
    30001 ctcagggaac ggcgcggcgg gtccggtggt ggacgccatt gaagcccgct ttaaagccct
    30061 cggcgcaccg gtggaattga tcaaagtgca taacacgccg gacggcaatt tccccaacgg
    30121 tattcctaac ccgttgctgc cggaatgtcg cgacgatacc cgcaatgcgg tcattaaaca
    30181 cggcgcggat atgggcattg cctttgatgg cgattttgac cgctgtttcc tgtttgacga
    30241 aaaagggcag tttatcgagg gttactacat tgtcggtttg ctggcagaag cgttcctcga
    30301 aaaaaatccc ggcgcgaaga tcatccacga tccgcgtctc tcctggaata ccgttgatgt
    30361 agtgacggcc gcaggcggca caccggtgat gtcgaaaacc ggacacgcct ttattaaaga
    30421 acgtatgcgc aaggaagacg ctatctacgg tggcgaaatg agcgcccacc attacttccg
    30481 tgatttcgct tactgcgaca gcggcatgat cccgtggctg ctggtcgccg aactggtgtg
    30541 cctgaaagga aaaacgctgg gcgaactggt gcgcgaccgt atggcggctt ttccggcaag
    30601 cggtgagatc aacagcaaac tggcacaacc tgttgaggcg attaaccgcg tggaacagca
    30661 ctttagccgc gaggcgctgg cggtggatcg taccgatggc atcagcatga cctttgccga
    30721 ctggcgcttt aatctgcgct cctccaacac cgaaccggtg gtgcggttga atgtggaatc
    30781 gcgcggtgat gtgccgctga tggaagcgcg aacgcgaact ctgctgacgt tgctgaacga
    30841 gtaatgtcgg atcttccctt accccactgc gggtaaggag ctaaaaacag gaacaacgat
    30901 gacaaatcta aaaaagcgcg agcgagcgaa aaccaatgca tcgttaatct ctatggtgca
    30961 acgcttttca gatatcacca tcatgtttgc cgggctatgg ctggtttgcg aagtgagcgg
    31021 actgtcattc ctctacatgc acctgttggt ggcactgatt acgctggtgg tgttccagat
    31081 gctgggcggc atcaccgatt tttatcgctc atggcgcggt gttcgggcag cgacagaatt
    31141 tgccctgttg ctacaaaact ggacgttaag cgtgattttc agcgccggac tggtggcgtt
    31201 caacaatgat ttcgacacgc aactgaaaat ctggctggcg tggtatgggc tgaccagcat
    31261 cggactggtg gtttgccgtt catgtattcg tattggggcg ggctggctgc gtaatcatgg
    31321 ctataacaag cgcatggtcg cggtggcggg ggatttagcc gccggacaaa tgctgatgga
    31381 gagcttccgt aatcagccgt ggttagggtt tgaagtggtg ggcgtttacc acgacccgaa
    31441 accgggtggc gtttctaacg actgggcggg caatctgcaa caactggtcg aggatgcgaa
    31501 agcgggcaag attcataacg tctatatcgc gatgcagatg tgcgacggcg cgcgagtgaa
    31561 aaaactggtc catcaactgg cggacaccac ctgttcggtg ctgctgatcc ccgatgtctt
    31621 taccttcaac attctccatt cccgtatcga agagatgaac ggcgtaccgg tggtgccgct
    31681 gtatgacacg ccgctttccg gggttaaccg cctgctcaaa cgtgcagaag acattgtgct
    31741 ggcgacgctt attctgctgc tgatctcccc ggtgctgtgc tgcattgcgc tggcggtgaa
    31801 actgagctca ccggggccgg ttattttccg ccagactcgc tacggcatgg atggcaagcc
    31861 gatcaaagtg tggaagttcc gttccatgaa agtgatggag aacgacaaag tggtgactca
    31921 ggcgacgcag aacgatccgc gcgttactaa agtggggaat tttctgcgcc gcacttcgct
    31981 ggatgaactg ccgcagttta tcaatgtgct gactggcggg atgtcgattg tgggtccacg
    32041 tccgcatgcg gtggcgcata acgaacagta tcgccagctc attgaaggct acatgctacg
    32101 ccataaagtg aaaccgggca ttaccggctg ggcgcagatt aatggctggc gtggcgaaac
    32161 cgacacgctg gagaaaatgg aaaaacgcgt cgagttcgac cttgagtaca tccgcgaatg
    32221 gagcgtctgg ttcgatatca aaatcgtttt cctgacggta ttcaaaggtt tcgttaacaa
    32281 agcggcatat tgatatgagc ttacgtgaaa aaaccatcag cggcgcgaag tggtcggcga
    32341 ttgccacggt gatcatcatc ggcctcggac tggtgcaaat gactgtgctg gcgcggatta
    32401 tagataacca ccagttcggt ttgcttaccg tgtcgctggt gatcatcgcg ctggcagata
    32461 cgctttctga cttcggtatc gctaactcca ttattcagcg aaaagaaatc agccaccttg
    32521 aactcaccac gctgtactgg ctgaacgtcg ggctggggat cgtggtgtgc gtggcggtgt
    32581 ttttgttgag tgatctcatc ggcgacttgc tgaataaccc ggacctggca ccgttgatta
    32641 aaacattgtc gctggcgttt gtggtgatcc cccacgggca acagttccgc gcgttgatgc
    32701 aaaaggagct ggagttcaac aaaatcggca tgatcgaaac cagcgcggtg ctggcgggct
    32761 ttacttttac ggtggttagc gcccatttct ggccgctggc gatgaccgcg attctcggtt
    32821 atctggtcaa tagtgcggtg agaacgctgc tgtttggcta ctttggccgc aaaatttatc
    32881 gccccggtct gcatttctcg ctggcgtcgg tggcaccgaa cttacgcttt ggtgcctggc
    32941 tgacggcgga cagcatcatc aactatctca acaccaacct ttcaacgctg gtgctggcgc
    33001 gtattctcgg cgcgggcgtg gctgggggat acaacctggc gtacaacgtg gccgttgtgc
    33061 caccgatgaa gctgaacccg atcatcaccc gcgtgttgtt tccggcgttc gccaaaattc
    33121 aggacgatac agaaaagctg cgcgtcaact tctacaagct gctgtcggtg gtggggatta
    33181 tcaactttcc ggcgctgctg gggctaatgg tggtgtcgaa taactttgta ccgctggtat
    33241 ttggcgaaaa gtggagcagc attattccgg tgctgcaatt gctgtgtatt gtggggctac
    33301 tgcgctccgt gggtaacccg attggttcgc tgctgatggc gaaagcgcgg gtcgatatca
    33361 gctttaaatt caacgtattc aaaacctttc tgtttattcc ggcaatcatc attggtgggc
    33421 agatggcggg cgcaatcggc gtcacgcttg gcttcctgct ggtgcagatt atcaacacca
    33481 ttctgagcta tttcgtgatg attaaaccgg tgctcggctc cagttatcgc cagtacatcc
    33541 tgagtttatg gctgccgttt tatctctcgc tgccgacgct ggcggtcagt tatgcgctgg
    33601 gcattgtgct gaaagggcaa ctggcgctgg ggatgctgct ggcggtgcaa atagccgcag
    33661 gcgtgctggc gtttgtggtg atgattgtgc tgtcacgcca tccgctggta gtggaagtga
    33721 agcgtcagtt ttgtcgcagc gaaaaaatga aaatgctttt acgggcgggg tgaatggctg
    33781 ttttcggttc cggcaacgaa cacaacaaac atagcctcac ctggcatcaa atttatgagg
    33841 aaaaaatgaa attacttatt ctgggcaacc acacttgcgg caatcgcggc gacagcgcca
    33901 tcttgcgcgg cttacttgat gccatcaaca tcctcaatcc acacgccgaa gtggacgtga
    33961 tgagtcgcta tccggtcagt tcttcctggc tgctcaaccg cccggtaatg ggcgatccgc
    34021 tgttcctgca aatgaaacaa cacaacagcg cggcgggcgt tgtcgggcgc gttaaaaaag
    34081 tcctccgtcg ccgctatcag caccaggtat tgctctcgcg cgtcaccgac actggcaagc
    34141 tgcgtaatat cgccatcgcc cagggattca ccgacttcgt gcgcttgctg tcaggctacg
    34201 acgccattat tcaggtcggc ggatcgtttt ttgtcgatct ctatggcgtg ccacagtttg
    34261 aacatgcgct ttgcacattt atggcgaaaa agccgctgtt tatgattggt cacagcgtcg
    34321 gccccttcca ggatgagcaa tttaaccaac tggcgaacta cgtttttggt cactgcgacg
    34381 cgctgatcct gcgcgaatcg gtcagccttg atttgatgaa acgcagcaat atcactaccg
    34441 caaaagttga acatggcgtt gatactgcct ggctggtcga tcatcacaca gaagaattca
    34501 ccgccagcta tgccgttcaa cactggctgg acgttgccgc acaacagaaa acggtggcaa
    34561 ttaccctgcg cgaactggca ccgttcgaca aacgtctcgg caccactcag caagcgtatg
    34621 aaaaagcctt tgccggggtg gtcaatcgca ttctcgacga aggctatcag gtgattgcgc
    34681 tctccacttg tacgggcatc gacagctata acaaagacga ccgcatggtg gcgctcaacc
    34741 tgcgccagca catcagcgat cctgcccgtt accacgtagt gatggatgaa cttaacgatc
    34801 tggaaatggg caaaattctc ggagcctgtg cactcactgt cggtacgcgc ctgcactctg
    34861 ccattatctc gatgaacttt gccactccgg cgattgccat caactatgaa cataaatccg
    34921 ccgggattat gcaacaactg gggctaccag agatggcaat tgatatccgt catttattag
    34981 acggcagcct gcaagcgatg gttgcggata ccttaggcca gcttccggca ctgaacacgc
    35041 gacttaacga agccgtcagt cgcgaacgtc agacgggaat gcagatggtg aaatctgtgc
    35101 ttgaacgcat cggggaggcg aaatgaaggt cggcttcttt ttactgaaat ttccactgtc
    35161 gtcggaaacc ttcgtcctca atcaaattac cgcgtttatt gatatgggct ttgaggtgga
    35221 gattgtcgcg ctgcaaaaag gcgacacaca aaacacccac gcggcatgga caaaatacaa
    35281 ccttgccgcc agaacccgct ggttacagga cgaacctacg ggcaaagtgg cgaaactgcg
    35341 ccaccgcgcc agccagacct tacgcggcat tcatcgtaaa aatacctggc aggcgctcaa
    35401 cctcaaacgc tatggtgccg agtcgcggaa cctgattttg tctgccattt gtggtcaggt
    35461 cgcaatacca ttttatgccg atgtctttat cgctcatttt ggcccggcgg gggtaaccgc
    35521 agcaaaacta cgcgaactgg gtgtcattcg cggcaaaatt gccactatct tccacggcat
    35581 tgatatttcc agtcgggaag tgctcaacca ttacactccc gaatatcaac aactgtttcg
    35641 ccgtggcgac ctgatgctac caataagcga tctgtgggcc ggaaggctgc aaaaaatggg
    35701 ctgcccgagg gaaaaaatcg ccgtatcgcg catgggcgtg gacatgacgc gttttagtcc
    35761 gcgtccggtg aaagcgcccg caacgccgct ggaaatcatt tccgtcgcac gcttaaccga
    35821 gaaaaaaggc ctgcatgtgg cgatcgaagc ctgccgtcag ttgaaagagc agggcgtggc
    35881 atttcgctat cgcatcctcg gcattggccc gtgggaacga cgcctgcgca ccctcatcga
    35941 acaatatcaa ctggaagatg tggtagagat gccgggcttt aaaccgagcc atgaagtgaa
    36001 agcgatgctc gacgatgcgg atgtcttcct cttgccatcg gttacaggtg cggacggcga
    36061 tatggaaggc attccggtgg cgctgatgga agcgatggcg gtcggcattc cggtggtttc
    36121 tactctgcat agcggaatac cggaactggt ggaggccgat aaatccggct ggctggtgcc
    36181 tgagaacgat gctcgcgcac tggcgcaacg actggcggcg tttagccaac tggacaccga
    36241 cgaactggct cctgtcatca aacgtgctcg cgaaaaagtc gaacacgatt ttaaccagca
    36301 ggtgattaat cgagaactcg ccagcttgct gcaggcttta tagaggagaa cgtatgccat
    36361 ttaaaacact ctcccgacgc accttcctga cggcaagctc cgcgcttgcc ttccttcata
    36421 cccctttcgc tcgcgcacta tccgcccgac aaagcgtaaa cattaacgac tacaaccctc
    36481 acgactggat cgcctcattt aaacaagcct tcagcgaagg gcaaacggtt gtcgtgcctg
    36541 gcggattggt ttgtgacaat atcaacaccg gcatctttat ccctcccggt aaaacgttac
    36601 acattcttgg aagcttgcgc ggcaacggca gagggcgatt tgtcttacag gacggcagcc
    36661 aggtgacagg ggaggagggc ggcagtatgc ataacatcac cctggatgtg cgcggctctg
    36721 actgcaccat caaagggctg gcgatgagag gctttggccc ggtaacgcag atttatatcg
    36781 gcggcaaaaa taaacgggtc atgcgcaacc tgaccatcga taacctgacc gttagccacg
    36841 ctaattacgc catcttacgc cagggatttc ataaccaaat aatcggtgcc aacatcacca
    36901 actgtaagtt cagtgactta cagggcgacg ctattgaatg gaacgtggcg attaacgacc
    36961 gtgatatttt gatatctgac catgtcatcg agcgcatcaa ctgtactaac ggcaaaatca
    37021 actggggcat tggcataggt cttgcgggaa gcacttatga taacaactac ccggaagacc
    37081 aggcagtgaa aaactttgtt gtggcgaata tcacgggatc ggattgtcgg caattgatcc
    37141 atgttgaaaa tggcaaacat tttgttatcc gtaatatcaa agcccgcaat atcacgccgg
    37201 atttcagtaa gaaagcaggt attgataacg ccacggttgc tatttacggt tgtgacaatt
    37261 tcgtgattga taatattgaa atgattaata gcgccgggat gttaattggc tatggggtaa
    37321 ttaaaggcaa atatctttcg ataccgcaaa atttccgagt gaataatatt caactggata
    37381 acacccatct tgcttataaa ttgcgcggca tccaaatctc cgccgggaat gccgtctcct
    37441 ttgtggcact gactaacatt gaggtgaagc gtgcgtcgct ggagttacac aacaaaccgc
    37501 aacatctttt tatgcgaaat atcaaagtga tgcaggaatc ctctgttgga cccgcattga
    37561 gcatgaactt cgacatgcgc aaagacgttc gcggcatgtt tatggcgaaa aaagaaacac
    37621 tgctgtctct tgcaaatgtt catgcggtga atgaaaaagg acaaagctcc gtcgatatcg
    37681 acaggattaa tcaccatatt gttaatgtgg aaaagattaa ctttagattg ccggaacgga
    37741 gagagtagat ttgcgaccat tcctggaaaa atggagccat acttaggaac aatgctactg
    37801 caatccacaa cgaagcggcg taacatcaca agtaattcag taatcaattc agggtaattg
    37861 atgctggcga aaaaaatcga acaagctata attcagcaac cattttacag gtggatgaaa
    37921 taatgacgaa tttaaaagca gttattcctg tagcgggtct tgggatgcat atgttgcctg
    37981 ccactaaggc gattcccaaa gagatgctac cgatcgtcga caagccaatg attcagtaca
    38041 ttgttgacga gattgtggct gcagggatca aagaaatcct cctggtaact cacgcgtcca
    38101 agaacgcggt cgaaaaccac ttcgacacct cttatgaatt agaatctctc cttgagcagc
    38161 gcgtgaagcg tcaactgctg gcggaagtac agtccatttg cccgccgggc gtgactatta
    38221 tgaacgtgcg tcagggcgaa cctttaggtt tgggccactc cattttatgt gcacgacctg
    38281 ccattggtga caacccattt gtcgtggtgc tgccagacgt tgtgatcgac gacgccagcg
    38341 ccgacccgct gcgctacaac cttgctgcca tgattgcacg cttcaacgaa acgggccgca
    38401 gccaggtgct ggcaaaacgt atgccgggcg atctctctga atactctgtc atccagacca
    38461 aagaaccgct ggatcgcgaa ggtaaagtca gccgcattgt tgaatttatc gaaaaaccgg
    38521 atcagccaca aacgctggac tcagacatca tggccgttgg tcgctatgta ctttctgccg
    38581 atatttggcc ggaactggaa cgcacgcagc ctggtgcatg gggacgtatt cagctgactg
    38641 atgccattgc tgaactggcg aaaaaacagt ccgttgatgc catgctgatg acaggtgata
    38701 gctacgactg cggtaaaaaa atgggttata tgcaggcatt tgtgaagtat ggactacgca
    38761 acctcaaaga aggggcgaag ttccgtaaag ggattgagaa gctgttaagc gaataatgaa
    38821 aatctgaccg gatgtaacgg ttgataagaa aattataacg gcagtgaaga ttcgtggcga
    38881 aagtaatttg ttgcgaatat tcctgccgtt gttttatata aacaatcagg ataacaacga
    38941 gttagcaata ggattttagt caaagttttc caggattttc cttgtttcca gagcggattg
    39001 gtaagacaat tagcgtttga atttttcgga tttggcgcga gtgggtaacg ctcgccacat
    39061 cgtagacatg catgcagtgc t
//
LOCUS       DADSZD010000023        38494 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.023, whole genome shotgun sequence.
ACCESSION   DADSZD010000023 DADSZD010000000
VERSION     DADSZD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.023"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            8..817
                     /locus_tag="J1Z28_001082"
     CDS             8..817
                     /locus_tag="J1Z28_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007669241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBA7715937.1"
                     /translation="MGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYPEYTVAG
                     RKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKLTGLNLAFGPFK
                     EWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNYGAANEN
                     EWDGYRFKIKYSIPLTNLFGGRLVYNSFTNFDFGSDLADKSHNNKRTSNAIASSHILS
                     LLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1035..3467)
                     /locus_tag="J1Z28_001083"
     CDS             complement(1035..3467)
                     /locus_tag="J1Z28_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBA7715938.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADNLISAF
                     VSDLEHLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3470..4630)
                     /gene="metB"
                     /locus_tag="J1Z28_001084"
     CDS             complement(3470..4630)
                     /gene="metB"
                     /locus_tag="J1Z28_001084"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBA7715939.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAENPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4907..5224
                     /gene="metJ"
                     /locus_tag="J1Z28_001085"
     CDS             4907..5224
                     /gene="metJ"
                     /locus_tag="J1Z28_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBA7715940.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5284..5892
                     /locus_tag="J1Z28_001086"
     CDS             5284..5892
                     /locus_tag="J1Z28_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBA7715941.1"
                     /translation="MKIRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAAGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDSSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(5953..6165)
                     /gene="rpmE"
                     /locus_tag="J1Z28_001087"
     CDS             complement(5953..6165)
                     /gene="rpmE"
                     /locus_tag="J1Z28_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBA7715942.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6368..8566
                     /gene="priA"
                     /locus_tag="J1Z28_001088"
     CDS             6368..8566
                     /gene="priA"
                     /locus_tag="J1Z28_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBA7715943.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVEAGSRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8722..9747
                     /gene="cytR"
                     /locus_tag="J1Z28_001089"
     CDS             8722..9747
                     /gene="cytR"
                     /locus_tag="J1Z28_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBA7715944.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9839..10798
                     /gene="ftsN"
                     /locus_tag="J1Z28_001090"
     CDS             9839..10798
                     /gene="ftsN"
                     /locus_tag="J1Z28_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBA7715945.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10891..11421
                     /gene="hslV"
                     /locus_tag="J1Z28_001091"
     CDS             10891..11421
                     /gene="hslV"
                     /locus_tag="J1Z28_001091"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBA7715946.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11431..12762
                     /gene="hslU"
                     /locus_tag="J1Z28_001092"
     CDS             11431..12762
                     /gene="hslU"
                     /locus_tag="J1Z28_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBA7715947.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTITIDADYVSKHLDALVADEDLSRFIL"
     gene            12829..13755
                     /gene="menA"
                     /locus_tag="J1Z28_001093"
     CDS             12829..13755
                     /gene="menA"
                     /locus_tag="J1Z28_001093"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBA7715948.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13848..14333
                     /gene="rraA"
                     /locus_tag="J1Z28_001094"
     CDS             13848..14333
                     /gene="rraA"
                     /locus_tag="J1Z28_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBA7715949.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14417..14662)
                     /gene="zapB"
                     /locus_tag="J1Z28_001095"
     CDS             complement(14417..14662)
                     /gene="zapB"
                     /locus_tag="J1Z28_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBA7715950.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15087..15932
                     /gene="glpF"
                     /locus_tag="J1Z28_001096"
     CDS             15087..15932
                     /gene="glpF"
                     /locus_tag="J1Z28_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBA7715951.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15955..17463
                     /gene="glpK"
                     /locus_tag="J1Z28_001097"
     CDS             15955..17463
                     /gene="glpK"
                     /locus_tag="J1Z28_001097"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBA7715952.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17598..18608
                     /gene="glpX"
                     /locus_tag="J1Z28_001098"
     CDS             17598..18608
                     /gene="glpX"
                     /locus_tag="J1Z28_001098"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBA7715953.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGVIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18705..19451
                     /gene="fpr"
                     /locus_tag="J1Z28_001099"
     CDS             18705..19451
                     /gene="fpr"
                     /locus_tag="J1Z28_001099"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBA7715954.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEIE
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLERFKNMVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRLPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19456..19884)
                     /gene="uspD"
                     /locus_tag="J1Z28_001100"
     CDS             complement(19456..19884)
                     /gene="uspD"
                     /locus_tag="J1Z28_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBA7715955.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19911..20210)
                     /locus_tag="J1Z28_001101"
     CDS             complement(19911..20210)
                     /locus_tag="J1Z28_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBA7715956.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(20422..20862)
                     /locus_tag="J1Z28_001102"
     CDS             complement(20422..20862)
                     /locus_tag="J1Z28_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA7715957.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            20963..21562
                     /locus_tag="J1Z28_001103"
     CDS             20963..21562
                     /locus_tag="J1Z28_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBA7715958.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            21670..22437
                     /gene="tpiA"
                     /locus_tag="J1Z28_001104"
     CDS             21670..22437
                     /gene="tpiA"
                     /locus_tag="J1Z28_001104"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBA7715959.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(22492..23247)
                     /gene="cdh"
                     /locus_tag="J1Z28_001105"
     CDS             complement(22492..23247)
                     /gene="cdh"
                     /locus_tag="J1Z28_001105"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBA7715960.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(23354..24343)
                     /locus_tag="J1Z28_001106"
     CDS             complement(23354..24343)
                     /locus_tag="J1Z28_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7715961.1"
                     /translation="MKKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKDTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(24663..25625)
                     /gene="pfkA"
                     /locus_tag="J1Z28_001107"
     CDS             complement(24663..25625)
                     /gene="pfkA"
                     /locus_tag="J1Z28_001107"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBA7715962.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(25806..26708)
                     /gene="fieF"
                     /locus_tag="J1Z28_001108"
     CDS             complement(25806..26708)
                     /gene="fieF"
                     /locus_tag="J1Z28_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBA7715963.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(26857..27357)
                     /gene="cpxP"
                     /locus_tag="J1Z28_001109"
     CDS             complement(26857..27357)
                     /gene="cpxP"
                     /locus_tag="J1Z28_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBA7715964.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            27507..28205
                     /gene="cpxR"
                     /locus_tag="J1Z28_001110"
     CDS             27507..28205
                     /gene="cpxR"
                     /locus_tag="J1Z28_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBA7715965.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            28202..29575
                     /gene="cpxA"
                     /locus_tag="J1Z28_001111"
     CDS             28202..29575
                     /gene="cpxA"
                     /locus_tag="J1Z28_001111"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBA7715966.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(29681..30355)
                     /gene="yiiM"
                     /locus_tag="J1Z28_001112"
     CDS             complement(29681..30355)
                     /gene="yiiM"
                     /locus_tag="J1Z28_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBA7715967.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(30504..31487)
                     /gene="kdgT"
                     /locus_tag="J1Z28_001113"
     CDS             complement(30504..31487)
                     /gene="kdgT"
                     /locus_tag="J1Z28_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBA7715968.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(31747..32367)
                     /gene="sodA"
                     /locus_tag="J1Z28_001114"
     CDS             complement(31747..32367)
                     /gene="sodA"
                     /locus_tag="J1Z28_001114"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBA7715969.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            32653..33687
                     /gene="rhaT"
                     /locus_tag="J1Z28_001115"
     CDS             32653..33687
                     /gene="rhaT"
                     /locus_tag="J1Z28_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBA7715970.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(33684..34622)
                     /gene="rhaR"
                     /locus_tag="J1Z28_001116"
     CDS             complement(33684..34622)
                     /gene="rhaR"
                     /locus_tag="J1Z28_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBA7715971.1"
                     /translation="MVFCNNANLLNVFVRHIANNQLRSLAEVATVAHQLKLLKDDFFA
                     SDQQAVAVADRYPQDVFAEHTHDFCELVIVWRGNGLHVLNDRPYRITRGDLFYIHADD
                     KHSYASVNDLVLQNIIYCPERLKLNLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVI
                     GQLEHESSQHVSFANEMAELLFGQLVMLLNRHRYTSDSLPPTSSETLLDKLITRLAAS
                     LKSPFALDKFCDEASCSERVLRQQFRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISD
                     ISTECGFEDSNYFSVVFTRETGMTPSQWRHLNSQKD"
     gene            complement(34606..35442)
                     /gene="rhaS"
                     /locus_tag="J1Z28_001117"
     CDS             complement(34606..35442)
                     /gene="rhaS"
                     /locus_tag="J1Z28_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBA7715972.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNYSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            35730..37199
                     /gene="rhaB"
                     /locus_tag="J1Z28_001118"
     CDS             35730..37199
                     /gene="rhaB"
                     /locus_tag="J1Z28_001118"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBA7715973.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            37196..38455
                     /gene="rhaA"
                     /locus_tag="J1Z28_001119"
     CDS             37196..38455
                     /gene="rhaA"
                     /locus_tag="J1Z28_001119"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBA7715974.1"
                     /translation="MTTQLEQAWELAKQRFTAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVARDQIKPEHFKNWVEWAKTNQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRAYEKEILSRRG"
BASE COUNT         9432 a        10051 c         9941 g         9070 t
ORIGIN      
        1 accttgcatt ggaaacgctc aggaaattaa aattgatgaa agctggttac atcaaagctt
       61 gaatgtcatt ggtcgcacag actctcgctt tggcccaaga ctgactaacg acctctaccc
      121 tgaatatact gtagcaggaa gaaaagactg gtttgatttt tatggttatg ttgatctacc
      181 gaaattcttt ggcgtcggca gtcactatga tgttgggatc tgggatgagg gctcaccact
      241 atttacggaa atagaacctc ggttttccat tgacaaattg accggattaa atcttgcgtt
      301 cggcccattt aaagaatggt tcattgcaaa caactatgtc tatgatatgg gtgacaacca
      361 gtcatcccgg caaagtacat ggtatatggg gcttggtaca gatatcgaca cgggtctacc
      421 aattaagctt tctgccaata tatacgccaa gtatcagtgg caaaactatg gtgccgctaa
      481 tgaaaatgaa tgggacggat atcgattcaa aataaaatat agcatccctc ttacaaattt
      541 attcggagga cgattggtat acaatagttt tactaacttt gattttggct ccgatcttgc
      601 ggacaagtca cacaataata aacgaaccag taatgctatt gcttcaagcc atatcctttc
      661 ccttctatat gaacactgga aatttgcatt tacactacgt tattttcaca acggtggaca
      721 atggaatgcg ggagagaagg ttaacttcgg agatggtcca tttgaattaa aaaatacagg
      781 atggggaacc tatactacta ttggttatca attttaaatt aggtgacatt ttagatattg
      841 ttacttaatg tcattttgta attgtcatta ataacttcac cattcactct gttacttcat
      901 cgctcaacac aaaagccccc ttattaccgg gggcactgac caaaggcaaa atgacaatca
      961 atcaccactt ccccaggaag aggtttaggg gtgacaacaa aagctggcaa ctgccagctt
     1021 ttgttcaata atcactacaa caactgcgcc agtcggttaa tatccgactg aatcgccccg
     1081 gcggtgacgt cgcgccctgc acctggcccg cggatcacca gcgggttgtc gcgataccag
     1141 cggctttcga tggcaaagac gttatcgcat ggcagtagtg atgccagcgg atggtcttca
     1201 cgcaccgctt ccacgcccac acgcgctttg ccgttggcat cgaaacgcgc cacgtagcgc
     1261 agcaccagcc ccatttcgcg ggccgcttcc agtcgttgca ccatctgctc gttaagttca
     1321 tcgccatttt caaagaaatg gtcgatgctg ccgccttcgc aatgggcagg taccagtgat
     1381 tcaacacgta cctggtccgg ttcaatgttg taacccgctt cacgcgccag aatcaccagc
     1441 ttgcgcatca cgtctttgcc ggaaaggtca tcacgcgggt caggctcggt taagccctgc
     1501 tgccacgcct gatccaccag ttcggtaaac ggcacgctac cgtcgaattg caggaacagc
     1561 caggagagcg tgccggagaa gatcccgctg atcgacaaaa tagtatcgcc gctgtcgatc
     1621 agatcgcgca cggtgtggtt gatcggcaaa cctgcgccga cggtagcgtt gtacagccag
     1681 tgacgcccgg ttttttcgaa ggcgtcgtgg atctggcgat atttattgct gtcgctcgct
     1741 cctgccagtt tgttggcact gatgacgtgg aaaccgtggc tggcgaaatc aagatactgg
     1801 tcagcgagct gctggctggc ggtaacgtcc agcaccacta aatcatcata cggatgggcg
     1861 cgcatccaca ggaacagcga ctcttcatcc tgctcgaccg cttcatcgtt gaagaaggct
     1921 agcgcgcggc tggcgtccag cccgtcatag ctcaacaggc tacggcggct gtccaccacg
     1981 cctgccagca caaattcaaa gccggtacgt gccgaaagcg tactctgctc acgggcgaac
     2041 agttccagcc agcgggaacc gatattgccc ttaccgaaca ataccaggcc gatgcgtttt
     2101 tccgcgcgga agacggactg atgcagcccc tgaatcaggc tttcggtcgg gccggtgcgc
     2161 agtactgcca ccaggctgat gccatcatca gactgccagg taaactcgac cggctggcct
     2221 ttcagttgct gccagaagcg gtggcaatgc agcgggttac gggtgacgcc cgccccaacc
     2281 atcgccacca gcgccagccc ctgacgcagg cgcagttcgc caggtaatcc cgcttcgtcg
     2341 aggattttca gcgcactgtc ggccacttct gaggtgtagc aaaattgcag caactggcga
     2401 tcgttatgca cgccaaccgc cagtgggcgc acctgagcac gtttcaggat ctggtcgatc
     2461 tctttatgcg ccagtttgaa atcctggctg gcgggcacct gaaactcaat caagcagaca
     2521 tcatcatggc tggtgacaat acgcgcacca gtaccggagg ccagcacgcg ttcaatgcgc
     2581 gtggaacctt gatccggcgt gtagctacag cgcagttgca ggtcgatttc gctgccagaa
     2641 acgggctgta aagtacgggc atgaagaacg ggagccgcca gacgcgccag ttcgctggct
     2701 tcatccagac gcagcaacgg cagcaggcag gcatctttca ctttacgcgg gtcggcgcta
     2761 tataccccgg cgacgtcact ccaaatggtt acgcgagaaa cacccgccag cgcaccgatt
     2821 tgtgtcgcgg aatagtcgga accgttacgc ccaagcagca ccgtttcacc ggcgttgtta
     2881 cggctgataa acccggtcac caccagacgt ttgcccggat gttgtaccag tagctgttgc
     2941 agcaacgggt acgaaagccc ttcatcaacc tgcggttgcg cagcgcgttc agcgcgtaaa
     3001 aactcgcggg catcaagcca ggccgctggc agcccttgtt gattaagcac cgcagacatc
     3061 aggcgcgccg accacacttc accgtggccc accacttccg catacaccgc atcgttaata
     3121 ccgctgtcga gcaacgcagc caggtgctca agatcgctga caaaagcgct aatgaggtta
     3181 tcggcttctt cggcgggtag caggccgcta atcagatcgc actgataacg acgtaatgtt
     3241 tgttgaacct gatgcgcaga gagacgatcg gtctggctta gtttcaacca gttaatcaac
     3301 tggttagtgg tgctaccggc ggcggaaacc accatcatat cgtcaggctg agagtactcc
     3361 gccataatgc ccgcgacacg caaataacac ttcacatcag ccagactact gccaccaaat
     3421 ttatgcagtt gacgaccttt cgcccctgcc tgcgcaatca cactcatttt taccccttgt
     3481 ttgcagcccg gaagccattt tccaggtcgg caattaaatc ttcgccatct tcaataccgg
     3541 tggagatacg cagcagcgtc tcggagatcc cggcggcagc acgcgcttct ggcgccatgc
     3601 ctgcatgtgt catggttgcg gcgtgagaga ttaaactttc cactccccct aatgattccg
     3661 ccagcgtaaa caacgacagc ccgcccagga aacgacgcag cgtctgctca tcgccatcca
     3721 gttcaaaact caacattgcg ccaaagcctt tttgctggcg cgcggcaatt tcatgaccct
     3781 gattttccgg cagcgacggg tgatacagtt ttttcaccaa cggctgggtt tgcagatatt
     3841 tcacaatcgc ctgcgcgttg cgctgcgcca gctccatacg cggcaccagc gttcgcaacc
     3901 cgcgtagcag cagatagctg tcaaacgcgc cgcccgtcac gccaatattg tttgcccacc
     3961 aggcaagttc agtgacaacg tccgggtctt tagcaatcac tacgcctgcc accacgtcag
     4021 agtgaccgtt cagatatttc gtgcatgaat gcaacaccag atcggcacct aatgccagtg
     4081 gattttgtaa tgccgggctt aagaaggtgt tatccaccac gctcaccgcc ccgacttccc
     4141 ttgccagatg gcagattttc gcaatatcca cgacgcgtaa caatggatta cttgggcttt
     4201 ctaccagtac cagtttgggg ttttctgcca gcgctgcccg taatgcctgt tcatcgcttt
     4261 gatcaacaaa caacacgcga tagcaaccgc gtttcgccag gctgtcgaac aggcgatagc
     4321 taccgccgta gcagtcgtgc ggcgcaacca gcagatcgcc aggtttcaaa aagacggtcg
     4381 ttaccaggtg aatcgcggac atgccggtat tagtaagtac tgcaccagca ccgccttcca
     4441 gttctgccag cgctcgctga accacatcgc gcgttgggtt gccgcgccgc gagtaatcat
     4501 gcgcgcgcgg ttcattaaat ccggtaaagt tataggtact ggaaagatgg atgggaggga
     4561 caacgcaacc atactgttcg tcgtcattta acccgctacg cactgcgatg gtggcctgtt
     4621 tacgcgtcat gtgatgaagt tccctgggct tggtcggtga aatgtcaggc accagagtaa
     4681 acattgtgtt aatggacgtc aatacatctg gacatctaaa cttctttgcg tatagattga
     4741 gcaaatctca aatagccgtt aaaattatat gcattatcac gccgacaggt gcattacacg
     4801 atgtcacggt aacgcctgta cggtaaacta tgcgggttta cggtcagtac ccatattaat
     4861 tgtgtggtct ggcctcaatt tattgacgaa gaggattaag tatctcatgg ctgaatggag
     4921 cggcgaatat atcagcccat acgctgagca cggcaagaag agtgaacaag tcaaaaaaat
     4981 tacggtttcc attcctctta aggtgttaaa aatcctcacc gatgaacgca cgcgtcgtca
     5041 ggtgaacaac ctgcgtcacg cgaccaacag cgagctgctg tgcgaagcgt ttctgcatgc
     5101 ctttaccggg caacctttgc cggatgatgc cgacctgcgt aaagagcgca gcgacgaaat
     5161 tccggaggcg gcaaaagaga tcatgcgtga gatggggatt aacccggaga cgtgggaata
     5221 ctaaagcgga aaagagcggc gcggagttaa ccagccgctt ttttcataca tggacgttta
     5281 acgatgaaaa tcaggctact gattctcagc ctgctggttt ctgtgcctgc atttgcctgg
     5341 caaccacaaa ccggcgacat catctttcag atctctcgct catcgcaaag taaagcgatc
     5401 caactggcga cccattccga ttacagccac accggtatgc tggtgatacg caacaaaaag
     5461 ccctacgttt ttgaagcggc cggcccggta aaatacaccc cactcaagca gtggatcgcc
     5521 catggtgaaa agggcaaata cgttgttcgc cgcgttgaag gcggactgag tgttgaacaa
     5581 cagcaaaaac tggcgcaaac ggcaaaacgt tatctcggta aaccgtatga ctccagcttc
     5641 tcgtggagcg acgatcgcca gtactgttcg gaagtggtgt ggaaggtcta ccagaatgcg
     5701 ctgggaatgc gtgtgggcga gcagcagaag ctgaaagagt tcgatcttag taacccgcta
     5761 gttcaggcaa agctcaaaga acgctacggc aaaaatattc cgcttgagga aacggtagtt
     5821 tcaccacaag ccgttttcga cgcgccacaa ctcaccacgg tcgccaaaga atggccgctg
     5881 ttttcctggt aatacgacca aaattcaggc acaaaaaaag cgccgtgcgg cgcttttttc
     5941 ggaaatccgg tcttatttgc tgcccgggat gttgaaacgc ttgttgaagc ggtcaacacg
     6001 gccaccggta gcaacatcac gctgtttgcc agtgaagaac gggtggcact tgctgcacac
     6061 gtcgaggttc aggtcatgac caacggtgga gcggattttc attacgttac cgcaagagca
     6121 gctagcagta atttcttcgt atttcgggtg aatatctttt ttcatgggaa aacctcggtt
     6181 taaggccgcg tcgctcttcc agccctaacg ccagacacca cgcgatgtta aaagtatagc
     6241 ttcaatacga tcatttcgta cgaaggcgcg aaatcataca gaaattaacc agcgtatgca
     6301 aactgatccg cactcttcta cggcaatgtg tatactaacc caccgaattt caagtcagga
     6361 tgatgctatg cccgttgccc acgttgcctt gcccgttccg cttcctcgta cctttgacta
     6421 tctgctgcca gaaggcatga cggttgaagc cgggagtcgc gtgcgcgtgc cgtttggcaa
     6481 acaacaggag cgtatcggtg ttgtggtgtc agtgagcgat gtcagtgagc tgccgcttaa
     6541 cgagctgaaa gcggtagttg aagtgctgga cgttgagccg gtatttactc actccgtctg
     6601 gcgattactg ctctgggcgg cagattacta tcatcatccg attggcgatg tgctgtttca
     6661 tgccttgccg attttactgc gccaggggcg gcctgcggcg aacgcgccga tgtggtactg
     6721 gtttgccact gaacaaggtc aggcggtgga tctgaacagc ctgaaacgct cacctaagca
     6781 acaacaggcg ctggcagcgt tacggcaagg caaaatctgg cgtgatcagg ttgccacgct
     6841 cgaatttaat gatgccgcgt tgcaggcgct gcgcaaaaaa ggtctgtgtg atttagcaag
     6901 tgaaacacca gagtttagtg actggcgaac gaactatgcc gtttctggtg agcggttgcg
     6961 attgaatacc gaacaggcca ccgccgttgg cgcaattcat agcgcggcag atactttttc
     7021 tgcctggctg ctggcgggcg ttaccggttc cggtaaaacg gaggtttatc tcagtgtact
     7081 ggaaaacgtg ctcgctcagg gcaaacaggc gctggtgatg gtgccggaaa tcggcctgac
     7141 accgcaaact atcgcccgtt ttcgtgaacg ttttaatgcc ccggtggaag ttctgcattc
     7201 cggcctgaac gacagcgagc gtctttcggc gtggttgaaa gcgaaaaatg gcgaggcggc
     7261 gattgtcatc ggcacccgct ccgcgctgtt tacgccgttt aaaaatctcg gcgtgattgt
     7321 tattgatgaa gagcacgaca gttcctacaa acagcaggaa ggctggcgct atcatgcccg
     7381 cgacctggcg gtgtatcgcg cgcatagcga gcaaatccca attattcttg gctccgcaac
     7441 gcccgcgctg gaaacgttat gcaacgtcca gcagaaaaaa taccgcctgc tgcgcctgac
     7501 ccgtcgggcg ggcaatgcgc gcccggcaat tcaacatgtg ctggatttaa aaggtcagaa
     7561 ggtgcaggca ggtctggctc cggcgttaat cacccgtatg cgccagcatt tacaggccaa
     7621 caatcaggtt atcctctttc ttaaccgccg gggttttgcg cctgcgctac tgtgccacga
     7681 ctgtggctgg atagccgaat gcccacgttg cgatcactac tacacgctgc atcaggcgca
     7741 acagcatttg cgctgccacc attgcgacag tcagcgtccg gtgccgcgcc agtgcccttc
     7801 ttgcggttcc acgcacctgg tacccgtggg gctgggcacc gaacagcttg aacagacgct
     7861 cgcgccgttg ttccctggtg tgcccatttc tcgtatcgac cgcgatacca ccagccgcaa
     7921 aggcgcgctg gaacaacaac tggcggaagt acatcgcggt ggcgcgagga ttttgattgg
     7981 cacgcaaatg ctggcgaaag gtcaccactt tccggatgtg acgctggtgg cattactgga
     8041 cgtggacggc gcgctgtttt ctgccgattt ccgctcggcg gagcgttttg cccagcttta
     8101 cacccaagtg gcaggccgtg ccggacgtgc cggtaaacag ggcgaagttg ttctgcaaac
     8161 gcaccatcct gaacatccgc tgttgcaaac gttgctctat aaaggctacg acgcctttgc
     8221 cgaacaggca ctggctgaac gacggatgat gcagctgccg ccgtggacca gccatgtgat
     8281 tgtgcgtgcg gaagatcata acaatcagca cgcgccgctg ttcctgcaac agttgcgtaa
     8341 tctgatcctc gccagcccgc ttgcagacga caaactgtgg gttctcggtc ccgttccggc
     8401 actggcacct aaacgtggcg gccgctggcg ctggcaaatt ttgttgcagc acccttcccg
     8461 cgtgcgatta cagcacatca tcaatggtac gctggcgctt attaatacaa taccggattc
     8521 ccgtaaggtg aaatgggtgc tggatgtcga tcccatcgag ggctaatgcg caaacgatgc
     8581 gaggcggatc gaaaaattca atattcatca cacttttcat gaaaattctg taaccgtttt
     8641 cacgcgctat ctgctaaaaa tgttgccgat gtgaagtaaa catggaagta gtacgcctgc
     8701 cgtgccaggc gaggagtgag tgtgaaagcg aagaagcagg aaactgccgc gaccatgaaa
     8761 gacgttgccc tcaaggcaaa agtctctaca gcgaccgtct cccgagcatt aatgaatccc
     8821 gataaagtct cccaggccac ccgtaatcgg gttgaaaaag cggcccggga agtgggttat
     8881 ttacctcagc ctatggggcg caacgtcaag cgtaatgaat cccgcaccat tctggtgatt
     8941 gtcccggata tctgcgatcc cttctttagc gaaattattc gcggtatcga agttacggcg
     9001 gcaaatcacg gatatctggt gctgattggc gactgtgcgc atcaaaatca gcaggaaaaa
     9061 acctttatcg atttgatcat caccaagcaa attgatggca tgttgctgct gggttcaagg
     9121 ctgccgtttg atgccagcat tgaggaacag cgtaatctgc cgccgatggt gatggcgaac
     9181 gaatttgcac cggaactgga gctgcctaca gttcatatcg acaatctgac cgccgcattt
     9241 gatgcagtaa attatttata tgagcaaggg cataaacgga ttggctgtat agccggtccc
     9301 gaagagatgc cgctgtgtca ctaccgcctg caaggctacg ttcaggcgct gcgtcgctgc
     9361 ggcattatgg ttgatccgca atacatcgcc cgtggcgact tcaccttcga agccggaagc
     9421 aaagcaatgc aacagctgct tgatcttcca caaccgccta ctgctgtctt ctgccatagc
     9481 gatgtgatgg cgctcggcgc actttcgcag gcaaaacgcc aggggctgaa agtaccggaa
     9541 gacctttcca taatcggttt tgataacatc gacctgacgc aattttgtga tccgccgctg
     9601 acaaccatcg cgcagccgcg ttacgaaatc ggtcgggaag ctatgctgtt attgcttgat
     9661 caaatgcagg ggcaacacgt tggcagtggc tctcgtttaa tggactgcga acttatcatc
     9721 cggggatcaa cacgcgcgtt accttaaagt aaacctttaa gactgccgtg actggtcaaa
     9781 ggcccgccgc ttaagtaaca tggcgggctg acgaacgaat aaatacagcg aaacgatagt
     9841 ggcacaacga gattatgtac gccgcagcca accggcacct tcgcggcgaa aaaagagcac
     9901 ctcacggaaa aagcaacgaa atctgcctgc ggtttctccc gctatggtcg ctattgctgc
     9961 cgccgttctt gtgaccttta tcggtggtct gtacttcatt acgcatcaca agaaagaaga
    10021 gtccgagacg ctgcaaagcc agaaagtgac cggaaacgga ctaccaccaa aaccagaaga
    10081 acgctggcgc tacattaaag agctggaaag tcgccagccg ggtgtgcgtg cgcccacaga
    10141 accttctgcc ggtggtgaag tgaaaacgcc ggagcaactg acaccagaac aacgtcagct
    10201 tcttgaacaa atgcaggctg atatgcgcca gcagccaacg cagctggttg aagttccgtg
    10261 gaatgaacag acgccggaac agcgccagca aacgctacag cgccagcgtc aggcgcagca
    10321 attagcggaa cagcaacgcc tggctcagca gtcgcgtacg actgaacaaa gctggcagca
    10381 gcagacgcgt acgtcgcaag ccgcgccggt acaggcgcaa ccgcgccagt ctaaaccggc
    10441 ttctactcag cagccttacc aggatctgct gcaaactcct gcacacacga ctgcgcagtc
    10501 gaagccgcag caagctgcgc cggttgctcg tgccgctgac gcgccaaaac cgacggcgga
    10561 gaaaaaagac gaacgccgct ggatggtgca gtgcggttcg ttcagaggcg cggaacaggc
    10621 agagacggta cgtgctcagc tggcgttcga aggctttgac tcgaaaatca ccaccaacaa
    10681 tggctggaat cgtgtggtca ttggcccggt gaaaggcaaa gagaacgcag acagcaccct
    10741 caatcgcttg aagatggcgg gtcatacaaa ctgcattcgg ctcgccgccg ggggttgaaa
    10801 ccctcaaaat cccccccatc tataattgca ttatgccccg tacttttgta cggggtttgt
    10861 actctgtatt cgtaaccaag gggtcagctc gtgacaacta tagtaagcgt acgccgtaac
    10921 ggccatgtgg tcatcgctgg tgatggtcag gccacgttgg gcaataccgt aatgaaaggc
    10981 aacgtgaaaa aggtccgccg tctgtacaac gacaaagtca tcgcgggctt tgcgggcggt
    11041 actgcggatg cttttacgct gttcgaactg tttgaacgta aactggaaat gcatcagggc
    11101 catctggtca aagccgccgt tgagctggcg aaagactggc gtaccgatcg catgctgcgc
    11161 aaacttgaag cattgctggc agtcgcggat gaaactgcat cgcttatcat caccggtaac
    11221 ggtgacgtgg tgcagccaga aaacgatctt attgctatcg gctccggcgg cccttacgcc
    11281 caggctgcgg cgcgcgcgct gttagaaaac actgaactta gcgcccgtga aattgctgaa
    11341 aaggcgttgg atattgcagg cgacatttgc atctatacca accatttcca caccatcgaa
    11401 gaattaagct acaaagcgta aggatctccc atgtctgaaa tgaccccacg cgaaatcgtc
    11461 agcgaactgg ataagcacat catcggccag gacaacgcca agcgttctgt ggcgattgct
    11521 ctgcgtaacc gctggcgtcg catgcagctc aacgaagagc tgcgccatga agtgaccccg
    11581 aaaaatatcc tgatgatcgg cccgaccggt gtcggtaaaa ctgaaatcgc ccgtcgcctg
    11641 gctaagctgg cgaatgcgcc gttcatcaaa gttgaagcga ccaaattcac cgaagtgggc
    11701 tatgtcggta aggaagtgga ttctattatt cgcgatctga ccgatgccgc cgtgaaaatg
    11761 gtacgcgtcc aggctatcga gaaaaaccgt tatcgcgctg aagaactggc agaagaacgt
    11821 attctcgacg tgctgatccc acctgctaaa aacaactggg gacagaccga acagcagcag
    11881 gaaccgtccg ctgctcgtca ggcattccgc aaaaaactgc gtgaaggcca gcttgatgac
    11941 aaagaaatcg agatcgatct tgccgcagca ccgatgggcg ttgaaattat ggctcctccg
    12001 ggcatggaag agatgaccag ccagctgcag tccatgttcc agaacctggg cggtcagaag
    12061 caaaaagcgc gtaagctgaa aatcaaagac gccatgaagc tgctgattga agaagaagcg
    12121 gcgaaactgg tgaacccgga agagctgaag caagatgcta tcgacgctgt tgagcagcac
    12181 gggatcgtgt ttatcgacga aatcgacaaa atctgtaagc gcggcgagtc ttccggtccg
    12241 gatgtttctc gtgaaggcgt tcagcgtgac ctgctgccgc tggtagaagg ttgcactgtt
    12301 tccaccaaac atgggatggt caaaactgac cacattctgt ttatcgcttc tggcgcgttc
    12361 cagattgcga aaccgtctga cctgatcccg gaactgcaag gtcgtctgcc aatccgcgtt
    12421 gagctgcagg cactgaccac cagcgacttc gagcgtattc tgaccgagcc gaatgcctct
    12481 atcactgtgc agtacaaagc actgatggca actgaaggcg taaatatcga gtttaccgac
    12541 tccggtatta agcgcatcgc ggaagcggca tggcaggtga acgaatctac cgaaaacatc
    12601 ggtgctcgtc gtttacatac cgttctggag cgtttaatgg aagagatttc ctacgacgcc
    12661 agcgatttaa gcggtcaaac tatcactatt gacgcagatt atgtgagcaa acatctggat
    12721 gcgttggtgg cagatgaaga tctgagccgt tttatcctat aatcgcgttc aatcattttc
    12781 atcattgttt gatggggctg aaaggcccca tttttattgg cgcgtattat gactgaacaa
    12841 caaattagcc gaactcaggc gtggctggaa agtttacgac ctaaaaccct ccccctcgcc
    12901 tttgctgcaa ttatcgtcgg gacggcgctg gcatggtggc aaggtcactt cgatccactg
    12961 gtcgccctgc tggcattgat taccgccggg ctattacaga tcctttctaa cctcgccaat
    13021 gattacggcg atgcggtaaa aggcagcgat aaacctgacc gcattgggcc gctacgcggc
    13081 atgcaaaaag gggtcattac ccagcaagag atgaaacggg cgctcattat taccgttgtg
    13141 ctcatctgtc tttccgggct ggcactggtt gcagtggcgt gccatacgct ggccgatttt
    13201 gtcggtttcc tgattcttgg tgggttgtcg atcattgccg ctatcaccta caccgtgggc
    13261 aatcgtcctt atggttatat cggtttgggt gatatttccg tactggtttt ctttggctgg
    13321 ttgagcgtca tggggagctg gtatttacag gctcatacat tgattccggc actgatcctt
    13381 ccagcaaccg catgcggcct gctggcaacg gcagtactga acattaataa cctgcgtgat
    13441 atcaatagcg accgcgaaaa cggcaaaaac acgctggtgg tgcgcttagg tgcagtaaac
    13501 gcgcgtcgtt atcatgcctg cctgctgatg ggctcactgg tgtgtctggc gctgtttaat
    13561 ctcttttcgc tgcatagcct gtggggctgg ctgttcctgc tggcggcacc gttgctggtg
    13621 aagcaagctc gttatgtgat gcgcgaaatg gacccggtgg cgatgcgacc aatgctggaa
    13681 cgcactgtca aaggagcgtt actgactaac ctgctgtttg ttttagggat attcctaagc
    13741 cagtgggcag cataactgac aaatatcaat taacaattga tgattttgcc aacagcccac
    13801 atagcgcgat atactgaaaa ttctcgcagc aactgaatgt taagcctatg aaatacgata
    13861 cttccgagct ttgtgacatc tatcaagaag atgttaacgt cgtggaaccg ctgttctcca
    13921 actttggcgg acgggcgtcg tttggcggac aaataatcac ggtaaaatgt ttcgaggaca
    13981 acgggttgct gtacgatctg ctcgaacaga atggccgtgg tcgtgttctt gtcgtcgatg
    14041 gcggaggttc tgttcgtcgc gcactggtcg atgctgaact ggcgcgtctg gcagtacaaa
    14101 atgaatggga aggtctggtc atttacggcg cggtgcgtca ggtagatgac ctggaagagt
    14161 tggatatcgg catccaggcg atggcggcaa ttccggttgg tgccgctggc gaaggcattg
    14221 gcgaaagcga tgtccgcgtc aattttggcg gtgtcacctt cttctccggc gaccatcttt
    14281 atgccgacaa taccgggatt attctttcag aagatccgct ggatattgaa tgataaaaaa
    14341 ggcaccgcaa ggtgcctttt ttctgcgtta cctgttggcc tacacagtaa agaaattacg
    14401 cgaaagatga atgtaatcag acctcttcca tgcgacccag cagggcctgt agacgttcct
    14461 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
    14521 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
    14581 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
    14641 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc
    14701 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
    14761 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgctacact
    14821 tttcatcctt ctcctggtga cataatccac accaatcgaa aatgttaata aatttgttgc
    14881 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
    14941 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa
    15001 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
    15061 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
    15121 ttgctgaatt cctcggtacc gggttgttga tttttttcgg tgtgggttgc gttgcagcac
    15181 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
    15241 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
    15301 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
    15361 cacaagttgc cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat
    15421 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
    15481 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
    15541 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggac gatggcaacg
    15601 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    15661 catctatggg cccattgacg ggctttgcca tgaacccagc gcgtgacttc ggtccgaaag
    15721 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    15781 acttcctggt gccgcttttt ggccctatcg ttggcgcgat tgtaggtgca tttgcctatc
    15841 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggagacca
    15901 caactccttc agaacaaaaa gcttcgctgt aatgtgacta cgggacaact aaacatgact
    15961 gaaaaaaaat atatcgtcgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtgatg
    16021 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    16081 ccgggctggg tggaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    16141 gaagtgctgg cgaaagccga tatcagttcc gatcagattg cagctatcgg tattacgaac
    16201 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    16261 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    16321 gattatatcc gtagcaatac cggtctggtg attgacccgt acttctctgg caccaaagtg
    16381 aagtggatcc tcgaccatgt ggaaggctcc cgcgagcgtg cgcggcgtgg tgaattgctg
    16441 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    16501 gattacacca acgcctcccg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    16561 aaaatgctgg aggtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    16621 gaagtgtacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    16681 atcgccggtg accagcaggc ggcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    16741 aagaacacct atggcactgg ctgctttatg ctgatgaaca ccggcgagaa agcggtgaaa
    16801 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    16861 ctggaaggtg cggtgtttat ggcgggcgca tccattcagt ggctgcgcga tgagatgaag
    16921 ttgattaacg acgcgtacga ttccgaatac tttgccacca aagtgcaaaa caccaacggc
    16981 gtatatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    17041 ggggcgattt tcggtctgac tcgtggggta aacgcgaacc acattatccg cgcgacgctg
    17101 gagtctattg cttatcagac gcgtgacgtg ctggaagcaa tgcaggccga ctctggtatt
    17161 cgactgcacg ccctgcgcgt ggatggcggc gcagtagcaa acaatttcct gatgcagttc
    17221 cagtccgata ttctcggtac ccgcgttgag cgcccggaag tgcgcgaagt caccgcattg
    17281 ggtgcggcct atcttgctgg tctggcggtt ggcttctggc agaatctcga cgagctgcaa
    17341 gagaaagcgg tgattgaacg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    17401 cgttacgcag gctggaaaaa agcggtcaaa cgcgcgatgg catgggaaga acacgacgag
    17461 taatgtaaat gccggatgaa gcgtttatgc cgcatccggt agtcctgaat cgtgcggggg
    17521 caaccccgca cacatcaata atcccgccct tcccctgtgc tacacttcgc gccattcctt
    17581 actgcttaga gtttgctatg agacgagaac ttgccatcga attttcccgc gtcaccgaat
    17641 cagcggcgct ggctggctac aaatggttag gacgcggcga taaaaacacc gcggatggcg
    17701 cggcggtaaa cgccatgcgt attatgctca accaggtcaa tattgacggc accatcgtca
    17761 ttggtgaagg tgaaatcgac gaagcaccga tgctctacat tggtgaaaaa gtcggtactg
    17821 gtcgcggcga cgcggtagat attgctgttg atccgattga aggcacgcgc atgacggcga
    17881 tgggccaggc taacgcgctg gcggtgctgg cagtaggcga taaaggctgc ttcctcaacg
    17941 cgccagatat gtatatggaa aagctgatcg tcgggccggg agcgaaaggc gtcattgacc
    18001 tgaatctgcc gttggcggat aacctgcgca atgttgcggc ggcgctcggc aaaccgttga
    18061 gcgaactgac ggtaacgatt ctggcaaaac cgcgccacga tgccgttatc gcagaaatgc
    18121 agcaactcgg cgtgcgcgta tttgctattc cggatggtga cgttgcggcc tcaattctca
    18181 cctgtatgcc ggacagtgaa gttgatgtgc tgtacggcat tggcggcgcg ccggaaggcg
    18241 tggtttctgc ggcggtgatc cgcgcattag atggtgacat gaacggtcgt ctgctggcgc
    18301 gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat tggcgagcag gagctggcac
    18361 gctgcaaagc gatgggcatc gaagccggta aagtattgcg tttgggcgat atggcgcgca
    18421 gcgacaacgt catcttctct gccaccggta ttaccaaagg cgatctgctg gaaggtatta
    18481 gccgcaaagg caatatcgcc accaccgaaa cgctgctgat ccgtggtaaa tcacgcacca
    18541 ttcgtcgcat tcagtccatc cactatctgg atcgtaaaga cccggaaatg caggtgcaca
    18601 tcctctgatt gatttgatcg attgagcctt ccagtccttc gggactggaa tttttttgtt
    18661 cggagaacga agataaggca agtcaatcaa aacaggagaa aaacatggct gattgggtaa
    18721 caggcaaagt cactaaagtg cagaactgga ccgacgccct gtttagtctc accgttcacg
    18781 ctcccgtgct tccgtttacc gccgggcaat tcaccaaact tggccttgaa attgaaggcg
    18841 aacgcgttca gcgcgcctac tcctatgtta actcgcccga taatccggat ctggagtttt
    18901 acctggtcac cgtccccgat ggcaaattaa gtccacgact ggcggcactg aaaccgggcg
    18961 atgaagtgca ggtggttagc gaagccgctg gcttctttgt gctggatgaa gtaccagatt
    19021 gcgaaacgct atggatgctg gcaaccggta cagcgattgg cccttattta tcgattctgc
    19081 aattgggcaa agatttagaa cgcttcaaaa acatggtgct ggttcacgcc gcacgatatg
    19141 ccgccgactt aagctatcta cctctgatgc aggaactgga aaaacgctac gaggggaaac
    19201 tgcgcattca gacggtggtc agtcgggaaa cggctgcggg ttcgctcact gggcgattac
    19261 cggcgttaat tgaaagtggt gaactggaaa gcgcggttgg cctgccgatg aataaagaaa
    19321 ccagccatgt gatgctgtgc ggcaatccac agatggtgcg cgatacacaa cagttgctga
    19381 aggagacccg gcagatgacg aaacatttac gtcgccgacc gggccatatg acagcggagc
    19441 attactggta agcggttact tgtcgataaa cggcacgatc aagagatccg cactcaactt
    19501 attgatcatc ccgcgatatg ccggcatcaa acggttgata aatgagtggt gatgaccaca
    19561 gacaaggagg tcgcactgct ctttttgcat aatttccagc agtgtttccg gcatttctcc
    19621 gcgttcaata cgcagttttg tcttcggcca ttgaatattt ttcgtcagtt tatacagctt
    19681 gttatccgac ttattcttca acaattgaag aatatcttct gttgcaggga agtagatacc
    19741 cggatacaac tcgcttaagc catcatcaat atgaattaac gtcaggtgag cgtcattatg
    19801 tctggcgagc tccagggctt tattcaccag taaggcatct tcttcattcc cggaaattgc
    19861 cacgccaatg tgtttataag ccatatttaa ctccttctaa agcaactcca tcaagctaac
    19921 gaacgcagtg ataactcaaa aataatcatc tcggcctggc atgagaaggt gaaggccgca
    19981 tcaagctcaa cttgcccttc cagatcctta atcttatagg ccacgctggc cccttcatct
    20041 gcagaggatg cagcggcggc aagctctttc agtttggtca taaaggactc tgcttcttcg
    20101 cgggtggcaa aaaagcgcga aaacttactg gtacagttgt cgttatcaat caccgtaccg
    20161 atatcaatcg cacatccttt agtactgcat ttatctacga catctttcat agggcacctc
    20221 tgtatattcg cctctctgtc gattacgact taagaataat gcgccgttaa aggaagaaca
    20281 aacaagtaat gatcttatgg attaccattt tttttgatat tgcttaaggt gaagagttca
    20341 gaaggatttt ccgggcattg tcacttgcat cgtggaaata acaatcccgg aaaaaaatag
    20401 cgataagaga tatattaaag gttaattact tgatttattg tcggctttat acttcacatc
    20461 ctgggtatct ttaccatatt tattttcgcc ttgtgtgcca acaaacgcac caagatcgat
    20521 aagcatcatc accagaatca atgtcgggac aaaacgcccc accgcccatt gccagacacc
    20581 cggtaaaatt gcccagttac ccgccagcag catccacgcc acaatcatca gaaatgccca
    20641 tgcgccggaa cgcccgcgat catgcaagcg cttaacggtt actgccgctg ttggccaaag
    20701 caagcacaca aggcaaaacg ccgcggtctg aatatcgagt aaatttttac ccgccagtga
    20761 gaaaagcacc agcatgcctg cgaaccacag gcctatccaa atccagaaat cacggcgtcc
    20821 aatacgccct ttaaatgaga ataaccattg ctgtatggtc atgtaagttc cttgatggtt
    20881 gtcttttcca ggattctacc cttttgacaa gggcgacaga tatcgtttta atcggagcca
    20941 gtcataacaa aaggtactgt caatgaagcc agggtgtacg ctgttttttc tcttatgttc
    21001 tgcattaacc gttacaacaa cggcgcatgc acaaacacca gatacggcaa cgaccgcgcc
    21061 ttatctgctg gctggagccc ctactttcga tctctccatc agccagtttc gagaagactt
    21121 taacagccag aatcccagcc tgccactgaa cgaatttcgt gccatcgaca gcagtcccga
    21181 caaagccaat ctcactcgtg cagcaagtaa aattaacgag aacttgtatg cttctactgc
    21241 gctggagcgc ggtaccttaa aaatcaaaag cattcaaata acctggctac ccatccaggg
    21301 gccagagcaa aaagccgcga aagcgaaagc tcaggcatac atggcagcgg tgatccgcac
    21361 actcacccca ttaatgacca aaactcaaag ccagaaaaaa ctgcagtcgc tactaacggc
    21421 ggggaaaaac aaacgttatt acaccgagac agaaggtgca ctgcgttatg ttgtcgcgga
    21481 caacggcgaa aaggggctga ccttcgctgt tgaaccgatt aagctggcgc tatctgaatc
    21541 gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg tgccgatgaa tctctatact
    21601 gtttcacaga cctgctgccc tgcggggcgg ccatcttcct ttattcgctt ataagcgtgg
    21661 agaattaaaa tgcgacatcc tttagtgatg ggtaactgga aactgaacgg cagccgccac
    21721 atggttcacg agctggtatc taacctgcgt aaagagctgg caggtgttgc tggctgtgcg
    21781 gttgcaatcg caccaccgga aatgtacatc gatatggcga agcgtgaagc tgaaggcagc
    21841 cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt ccggcgcatt caccggtgaa
    21901 acctctgctg ctatgctgaa agacatcggc gcacagtaca tcatcatcgg ccactctgaa
    21961 cgtcgtactt accacaaaga atctgacgaa ctgatcgcga aaaaattcgc ggtgctgaaa
    22021 gagcagggac tgactccggt tctgtgcatc ggtgaaaccg aagcggaaaa cgaagcgggc
    22081 aaaactgaag aagtttgtgc acgtcagatc gacgcggtac tgaaaactca gggtgctgcg
    22141 gcattcgaag gtgcggttat cgcttacgaa cctgtatggg caatcggtac tggcaaatct
    22201 gcaactccgg ctcaggcaca ggctgttcac aaattcatcc gtgaccacat tgctaaagtt
    22261 gacgctaaca tcgctgaaca agtgatcatt cagtacggcg gctctgtaaa cgcgtctaac
    22321 gctgcagaac tgtttgctca gccagacatc gacggcgcgc tggttggcgg tgcttctctg
    22381 aaagctgacg ctttcgccgt gatcgttaaa gctgcagaag cggctaaaca ggcttaagtc
    22441 tgacaggtgc cggatttcat atccggcact tactttccta aactcctcgc cttaacgcaa
    22501 aatctcacac tgatgatcct ggatttcttc ggctgaagca cggttcagcg tcagtagatt
    22561 tcgttgtgtc gccagcaata caaatgagtt atcactctgc cgtaccatcg ccagcccgta
    22621 gcttcccata tgttcccgcg cctcaggcac ttcttccgcc agcatcataa atgggctgcg
    22681 ttgtaccagt tcgctttccg ttacccgacg cgccaggtac tcatgcccac gcaaaccacc
    22741 tggcagtggc aaccagcggc tactgatgtt cgccagattg ttatccagct gttcgcgcac
    22801 atcaggacga atacaagaaa tatgaatatg aaaatggttt tgcgtacgcc cggtgcggga
    22861 gttgatcgcc agagataccg cgcgatcggg aacaggctgg ccgtattttt ggctcataaa
    22921 atcacgcgcc tgccaggcta accagaaaaa attaggcgtt gacggatcgg tcaacaatgg
    22981 gctttcagta ccgttaatac ggtacgttgg catcaacaga tattgcagtg ggccatgacg
    23041 atcttttaaa accacgtatc cggcgttggg tttgacttcc gcacatggcg acgggttttg
    23101 gttttcctgc tggttgggca aacattgctc aagaacaatc ttacgtaacg tgtctgactc
    23161 ttcaccggtt aatttccagt aaccaatacc ggcagccaca acggcgataa ctatcatcac
    23221 caaaaaaaga agacccgctt ttttcatctt tttttccctg tacctcaaag agatgcaagg
    23281 gtaacgcaaa atcgtgacaa ataaaaaacc cagtggcgag aaaccaccgg gcttgataat
    23341 tatcctgggg aaatcagcgt ttgctgatct gatcgaacgt accgccgtta gcaaaatgct
    23401 ctttttgcgc tttcgtccag ccgccgaact cttcatcaat ggtgaataac ttcaattttg
    23461 gaaacgcatt ttcgtacttt ttcgctacct cagcgtcgcg cggacggtag tagtttttcg
    23521 ctgcaatttc ctgaccttct ggcgagtaga gatatttcag gtaggcttcc gccacctctt
    23581 tggtgtcttt tttctcgacc actttatcga ccaccgacac ggttggctct gcgaggatag
    23641 actcactcgg cgtgacgatt tcgaatttat ctttccccag ttcattcgct gccagcagag
    23701 cttcattttc ccaggcaatc agtacatcgc caattccgcg ctcgacaaaa gtgttagtgg
    23761 atccgcgcgc gccagaatcc agaacttcga cgtttttata cagtgcccga acaaaatcct
    23821 gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta gccccaggct gccaggtaat
    23881 tccagcgcgc gccaccagag cttttcggat taggcgtgat caccgaaaca cccggtttaa
    23941 tcagatcgtt ccagtcatgg atctgcttcg gattaccctt acgtaccagg aaaacaatgg
    24001 tggaagtgta cggtgcggag ttatccggta gacgtttgat ccactcttta tcaatccgcc
    24061 cgcgttccgc aattgcgtcc acgtcatagg ccagagccag cgtgacaaca tcagcttcaa
    24121 taccgttgat taccgacgtc gcttgtttgc ctgaaccacc gtgtgactga cgaatcacca
    24181 cgttatcgcc agtttgctgt ttccagtggg cgctgaatgc cttgttgtac tgttcgtaca
    24241 attcgcgcgt tggatcatat gaaacgttaa gaagctgaat atcctttgcc ataacgctgg
    24301 ttgccgccag caaaaatgtt aaccctacgc cccacttttt catcgcccga ctctcttatg
    24361 ttgtgttgtg atgagcaaag cgtgccagaa ggttgaccaa acattaaaga ataaaaaaag
    24421 attggctata acttgcgagt atatgttggg ggattaaaaa ggcggaagaa atccgcctca
    24481 tattgctgac aaagtgcgct ttgtccatgc cgaatgcggc acaaacgcct tatcctgcct
    24541 acaaaagttt gcaaattcaa tagattacag aattcatgta ggcctgataa gcgaagcgca
    24601 tcaggccttt ttgcttctgt catcggtttc aggctaaagg aatctgcctt tttccgaaat
    24661 cattaataca gttttttcgc gcagtccagc cagtcgcctt tgaacggacg cttcatgttt
    24721 tcgatagcgt cgatgatgtc gtggtgaacc agctgttcgt tctggatacc tacgcaacga
    24781 ccaccgtaac ctgccagcag cagatcgata gcgtaagcgc ccatacggga agccagaata
    24841 cggtcgtaag gcaccggaga accaccgcgc tggatgtggc ccagcacagt tgcgcgggtt
    24901 tcacgaccgg tttctttctc gatgaaatgc gccagttcgt caacatcaca catatgttcg
    24961 gtaatcgcca cgatcgcgtg ttttttacct ttcgcgatac ccgctttgat ttcgtttacc
    25021 aggtcttcac ggctgaattc aacttccgga accacaacga attcacagcc gccggcaata
    25081 gccgcagcca acgtcagatc gccacaataa cggcccatca cttccaccac ggaaatacgc
    25141 tggtgagaag aagaggtgtc acgcagacgg tcgatcgctt ctacaacggt gctcagcgca
    25201 gtgaagaaac cgatagtgta gtcagtgcct ttgatgtcgt tgtcgatagt gcccggcagg
    25261 ccgatgcacg ggaagcccat ttcggtcaga cgcattgcac ccatgtagga accgtcaccg
    25321 ccgataacca ccagcgcgtc gatcccacgt tttttcaggt tttcgatagc cacggcgcgg
    25381 atgttctcgt cgcggaattc cgggaaacgc gcagaaccga ggaacgtacc accacggttg
    25441 atcatgtcag aaacgctgta acggtctagc tgtaccatac ggtcttcata cagacccaga
    25501 tagccgtcat aaatgcccat tacttccaga ccttctgtca gcgcagaacg aacaaccccg
    25561 cgaattgcgg cgttcatgcc tggcgcatca ccgccgcttg tcaacacacc gattttctta
    25621 atcatgacta cctctgaact ttggaatgca aaatgaaatc tgttgccgga agtcttcttg
    25681 cacatcgaag tgatccaacg aatgtgcaaa tagtataaca atcacttcct gctgaattga
    25741 ttcaggtcag gccaaatggc ggtattttat acacaaaatg cgggtctggc tctcttttat
    25801 actgattatg aaagcataga ccgtttaccc tccctgggta cgacggaaca ggggtcctga
    25861 tggataatca catccgatcc cggaaaacgc cgtaaaatag cctgctctac ctgatccgcc
    25921 accatatgtg cctgaaccaa aggcagagag tcttccattt ccaaatgaat ctgaataaag
    25981 cgggtcggcc ctgactgccg cgtgcgaaga tcgtgagcgc cgctaacacc cggccaggaa
    26041 gtcacgatat caataatttc ttgccgttcc tcatcaggca atgcgcgatc cagtaatgac
    26101 tgtaccgcct catatcccat gcgtaacgcg ctatataaaa tatagatgcc gatccccaat
    26161 gcaaacagag catcggcgcg atgccagccg taccaggaca accccagcgc cagcagaatt
    26221 gcgccgttca tcataacatc agactggtaa tgtagcatat cagcccgcac cgcctggctt
    26281 tgcgtccggc gcaccaccca acgctgaaac gagacaagga taatcgtaca aattagcgcc
    26341 acaattgtca cgataacccc gacgcctgga tctgtcatcg gtgttggaga taccagatgt
    26401 tgaatacccg tcaaaaacag gaatagtgcc gaaccggaga taaacatact ttgcgccagc
    26461 gccgcgaggg actcagcttt accgtgacca aacgagtgat tatcgtcggc aggttgcagg
    26521 gaatatcgca ccaccagtaa attcgtcaac gacgcgccga tatccaccag cgaatccacc
    26581 agcgcggcga gaatactcac cgacccggta taccaccatg caaaaatttt aatcagcaat
    26641 agcagcgaag ccatcgccgt cgcagcaatc gccgcccgac tgaccagccg tccataagat
    26701 tgattcataa atactcccgc tatcaactga cggtagtata acggaagcaa atcatctgca
    26761 atgcattaag cagcaggcaa attgaggata aaaaaaaccc ccacatcatg tgggggaaga
    26821 cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact
    26881 actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcgcgca actgctccat
    26941 tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat
    27001 ttggttgcgg acttttgcca tctcaacctg acgagctatt tgctcgttcg ccattttttc
    27061 tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt
    27121 ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg
    27181 catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact
    27241 gcgctgcgta agttcttcac ccggatgcca gttatcgcct gaaccgactt cagcagcgtg
    27301 gcttaatgaa ctgactgcca gcgttgaggc catgacggca gcggtaacta tgcgcatcat
    27361 ttgctcccaa aatctttctg tcgcgattca acgatagaga gtttacgatt caggctgcaa
    27421 acatgcgtca gggggtgtaa aacaacgtaa agtcatggat tagcgacgtc tgatgacgta
    27481 atttctgcct cggaggtatt taaacaatga ataaaatcct gttagttgat gatgaccgag
    27541 agctgacttc cctattaaag gagctgctcg agatggaagg cttcaacgtg attgttgccc
    27601 acgatgggga acaggcgctt gatcttctgg acgacagcat tgatttactt ttgcttgatg
    27661 taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc acttcgccag acacaccaga
    27721 cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga tcgcgttctc ggccttgagc
    27781 tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg tgagctggtg gcacgtattc
    27841 gcgcgatcct gcgccgttcg cactggagcg aacaacagca aaacaacgac aacggttcac
    27901 cgacactgga agttgatgcc ttaagtctca atcctggtcg ccaggaagcc agcttcgacg
    27961 ggcaaacgct ggagttaacc ggtactgagt ttaccctgct ctatttgctg gcacagcatc
    28021 tgggtcaggt ggtttcccgt gagcatttaa gccaggaagt gctgggcaag cgcctgacgc
    28081 ctttcgaccg cgctatcgat atgcacattt ccaacctgcg tcgtaaactg ccggatcgta
    28141 aagatggtca cccgtggttt aaaaccctgc gtggtcgcgg ctatctgatg gtttctgctt
    28201 catgataggc agcttaaccg cgcgcatctt cgccatcttc tggctgacgc tggcgctggt
    28261 gttgatgttg gttttgatgt tacccaagct cgattcacgc cagatgaccg agcttctgga
    28321 tagcgaacag cgtcagggtc tgatgattga gcagcatgtc gaagcggagc tggcgaacga
    28381 tccgcccaac gatttaatgt ggtggcggcg tctgttccgg gcgattgata agtgggcacc
    28441 gccaggacag cgtttgttat tggtgaccac cgaaggccgc gtgatcggcg ctgaacgcag
    28501 cgaaatgcag atcattcgta actttattgg tcaggccgat aacgccgatc atccgcagaa
    28561 gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc gtgcgtgatg gcgaagataa
    28621 ttaccaactt tatctgattc gtccggccag cagttctcaa tccgatttca taaacttact
    28681 gtttgaccgc ccgctattac tgctgattgt caccatgctg gtcagtacgc cgctgctgtt
    28741 gtggttggcc tggagtctgg caaaaccggc gcgtaagctg aaaaacgctg ccgatgaagt
    28801 tgcccaggga aacttacgcc agcacccgga actggaagcg gggccacagg aattccttgc
    28861 cgccggtgcc agttttaacc agatggtcac cgcgctggag cgcatgatga cctctcagca
    28921 gcgtctactt tctgatatct ctcacgaact gcgcaccccg ctgacgcgtc tgcaactggg
    28981 tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa ctggagcgta ttgaaactga
    29041 agcgcaacgt ctggacagca tgatcaacga tctgttggtg atgtcacgta atcagcagaa
    29101 aaatgcgctg gttagcgaga ccatcaaagc caaccagttg tggagtgaag tgctggataa
    29161 cgcggcgttc gaagccgagc aaatgggcaa gtcgttgacg gttaacttcc cacctgggcc
    29221 gtggccgctg tacggcaacc cgaacgccct ggaaagtgcg ctggaaaaca ttgttcgtaa
    29281 tgctctgcgt tattcccata cgaagattga agtgggcttt gcggtagata aagacggtat
    29341 caccattacg gtggacgacg atggtcctgg cgttagcccg gaagatcgcg aacagatttt
    29401 ccgtccgttc tatcggaccg acgaagcacg cgatcgtgaa tctggcggta caggtttggg
    29461 gctggcgatt gttgaaaccg ccattcagca gcatcgtggc tgggtgaagg cagaagacag
    29521 cccgctgggc ggtttacggc tggtgatttg gttgccgctg tataagcgga gttaaactcc
    29581 gcatttgtag gcaggataag gcgtttacgc cgcatccggc atttgagcag gatgcctgat
    29641 gcgacgctga tagcgtctta tcaggcctac actccggggg ttattttccc cacaatcggc
    29701 gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg catcgttcgc gtccagcttt
    29761 tcgataaccc tgcggcggag agtaaacggt gatactgatc gtcatcaaac ggcatatgcc
    29821 atgcgatggc ggcagcctcc tgcacggtca catcgctgac acgggaaacc aactccagtg
    29881 gcgcatcagc agataccaac cctggtgcaa ttacgctata caaccacccc actttacccg
    29941 tgttttgcat caactgcgct atatcgctga tatcaaaatg gtaattgagt ttatagcaag
    30001 gtgagcgtgg ctggctgacc tgaattaatg cctctcccca gcggaaaata tcaccaatat
    30061 aaacattact ttccgtcaga ccgtcggttg agagattttc gccaaacgca ggcgcaacaa
    30121 acagctccgc ctgttccgga aattcccgcg cccagtagag ataatgctca cgaggataat
    30181 gacacagcgc tctgtctggc ccgccgtgga tctttttctc cgcctgttcg tcaccttcca
    30241 gccccaggtc cgtcagcatc aactcaccat caacctggat tttggcaatt gcgctgggtt
    30301 tgccttcggg ataagcctga atcttgcctg tgtatacatc aaccggatat cgcatctgtt
    30361 ccctctccac gagaaatttc cccattagac catatttatt ttatgtcagg cgtgctgttt
    30421 tcgcggtaag tatccaggca gaaacgcaat tgaaaaccgg acgaacgtta gttcatccgg
    30481 ctgtggggat taaggaagtt ggtttatttc accgtaccta aaatttcgat tttcgccgct
    30541 ctggctttga ctttacgtga ccagatagag gtaagaattg gcaccagaat cgaagtcaca
    30601 atgaccgccg tcgctaccag cgaagttgct gccggagcca taggtttaaa cgcaggcacc
    30661 atttctgcaa tcagcacagg tgtcgctacc gcggcccctg cggaactgga agcggcaatt
    30721 ccggcagtgc catcgccacc gccaatcaat ttatcggcga taatcaacgg aataccggtc
    30781 acgataatta ctgccacacc caacaggatc cccagcaaac ctgtctgggc aattacagtc
    30841 aaatcaatgg tattgcccag tgcgaaggca aagaatggaa tcagcgtctg caccgctttg
    30901 ctaaaaaatt ctcgtaactc agggtcaagg ttccccaggg caaagcccac caggaacggc
    30961 aatactgcgc cgacgaaaac atgcggttca aacgaggcaa tcccggcagt gcccagaata
    31021 atcatcgtca tgagcggccc ggactccaac gacatcaaca caaatgcccc agcttcttct
    31081 tttgtgccgt actgctgcat gatggaagcg taaagtccgc cgttggtcat atccatcgcc
    31141 gccaccagcg ccaacgttga aagtccggca aaaaatccaa cttcaacacc atgttccgga
    31201 ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg caattttagt taccaccagc
    31261 gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt ttattgacgc ccccatgcaa
    31321 aaaaaccaca ccgccagaat gggcaccgta ccggtaatca tcccgttggt gaatgatcca
    31381 aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg cgccaaggaa tagcgggacg
    31441 agcatcatcc cccccgggat tttctcaatc gagcgtttta tctgcatttc catcacctgt
    31501 cggcatattt ttaagggctg atgcgtcatt cctcaacgga gcatcagtgt tgatggaaac
    31561 gaagatagac attcatttga tcaataaaag tgatcaaaac cacaccaatt gaaacgctgt
    31621 tttattttta tcggatcatt gtttttattt tgaaattgat cacaaaaaaa ccaccgccgt
    31681 tggcgatggt tcatttttta agctgcaatg cggcgtaaac gcctcattgc agcaggcggc
    31741 aaatgattat tttttcgccg cgaaacgtgc cgctgcttcg tcccagttca ccacgttcca
    31801 gaactcttta atgtagtccg gacggcggtt ctggaatttc agatagtaag catgttccca
    31861 cacatccagg cccagaatcg ggaagccgga agcgccagaa atggcttcgc ccatcagtgg
    31921 agagtcctgg ttagcagtag aaaccaccgc cagtttatcg cctttttgca ccagccatgc
    31981 ccagccggaa ccaaagcggg aagctgccgc tttttcaaat tctgctttga aattatcaac
    32041 ggaaccgaaa tcacgttcga tagccgcttt caggtcgccc tgcagggtgg tgcctttttt
    32101 cagacctttc cagaacagac tgtggttagc gtgaccacca gcgttattgc gcagtacggt
    32161 tttcttgtct gctggcagct ggtccagttt ggtgatcagc tcttcaaccg gcaggttggc
    32221 aaattctggc aggttttcca gcgccgcgtt ggcgttgttg acgtaagtct gatggtgttt
    32281 ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt tccagggcat cgtaagcata
    32341 cggcagggat ggcagggtat aactcatatt catctccagt gttgtcgggc ggccgattgt
    32401 taatgccgcg taagcagttg gttcattata gttaattaaa tgatattgaa aatgattatc
    32461 aatgccgtac ttttcataat ggtatggttt tgcaggaaaa tgcccaagat gtgaagcaaa
    32521 tcacccactt aatgccgtga ttgccagtaa atcggcagcg gcggcaacag gcgaaaggtt
    32581 aatcgacagc tcaattttta cactcatctc gtcggagatg tgacgcgacg aaaaaatgat
    32641 gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg
    32701 gccagtgcag cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc
    32761 atgtggtcag tcggtgggat tgtttcgtgg attatcctgc cgtgggccat cagcgccctg
    32821 ttactaccga atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt
    32881 tttctgttcg gcgctatgtg ggggatcggt aatatcaact acggcctgac catgcgttat
    32941 ctcggcatgt cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg
    33001 atgacgccaa ttatcaacgg caatttcgat gtgctgatta ataccgaagg cggacgcatg
    33061 acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga ttgtaactcg cgccgggcag
    33121 ttgaaagagc gcaagatggg cattaaagcc gaagagttca atctgaaaaa agggctggtg
    33181 ctggcggtga tgtgcggcat tttctctgcc gggatgtcct ttgcgatgaa cgccgcaaaa
    33241 ccgatgcatg aagccgctgc cgcgctgggc gtcgatcccc tgtatgtcgc tctgccaagc
    33301 tatgttgtca tcatgggcgg cggcgcaatc atcaacctcg gcttctgctt cattcgtctg
    33361 gcaaaagtga aagatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt
    33421 cacaatgtgt tactctcagc actgggcggg ttgatgtggt atctgcaatt ctttttctat
    33481 gcctggggcc acgcccgcat tccggcgcag tatgactaca tcagctggat gctgcatatg
    33541 agcttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaatgca
    33601 ggacgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgctaac
    33661 atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg
    33721 ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca
    33781 cattcggtcg aaatatcact gattaacagg cggctatgct ggaggagata ttgcgcatga
    33841 cacactctga cctgtcgcag atattgattg atggtcattc cagtctgctg acgaaattgc
    33901 tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga
    33961 cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt
    34021 ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac
    34081 agcaactcag ccatttcgtt agcaaacgac acatgctgac tactttcatg ctcaagctga
    34141 ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct
    34201 gcgctggcgc taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc
    34261 gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg
    34321 tcagcatgaa tataaaagag atcgccacgg gtaatgcgat aaggacgatc gttgagtaca
    34381 tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca
    34441 aagacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa
    34501 tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt
    34561 tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaaacc
    34621 atcccgtccc tgacgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga
    34681 aaagtggtta ctgtcgctga atccgcagcg ataggcgatg tcagtaacgc tggcctcgct
    34741 gtggcgtagc agatgtcggg ctttcatcag tcgcaggcgg ttcaggtaac gctgaggcgt
    34801 cagccccgtt tgctgcttaa gctggcgatg cagcgtacgc aatgaaagag aaaattgatc
    34861 cgccacagcg tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa
    34921 gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag
    34981 caataattgc ataaataaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc
    35041 ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt agttaacacg
    35101 ccagtgagac ggatactgcc catcctgctc ttgtggcagc aactgattca gcccggcgag
    35161 aaactgaaat cgatccggcg agcgatacag cacattggtc agacacagat tatcggtatg
    35221 ttcatacaga tgccgatcat gatcgcgtac gaaacagacc gtcccaccgg tgatggtata
    35281 gggctgccca ttaaatacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa
    35341 atcatgatga tgttcaggaa aatccgcctg cggaagccgg ggttctatcg ccacggacgc
    35401 gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg
    35461 tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa
    35521 ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcagaaaa tgcggtgagc
    35581 atcacatcac cacaatgaag caaattgtga acatcatcac gttcatcttt ccctggttgc
    35641 caatggccca ttttcctgtc agtaacgaga aggtcacgaa ttcaggcgct ttttagactg
    35701 gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc
    35761 tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga
    35821 cgctgcgcga aatccatcgt tttaacaatg gcctgcatag ccagaacggt tatgtcacct
    35881 ggaatgtgga tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag
    35941 ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc
    36001 aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctaa
    36061 tggcgcaggc acaacaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt
    36121 ttctgccctt caatacgatc tatcagctac gtgcactgac agagcagcaa ccagaactta
    36181 ttccacacat tgctcacgct ttgctgatac cggattactt tagttatcgc ctgaccggca
    36241 agatgaactg ggaatatacc aacgccacga ccacgcaact ggtcaatatc aatagcgacg
    36301 actgggacga gtcgctactg gcgtggagcg gtgccaacaa agcctggttt ggtcgcccga
    36361 cgcatccggg taatgtcata ggtcactgga tttgcccaca gggtaatgag attccggtgg
    36421 tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac
    36481 gcgccgctta tctctcttct ggcacctggt cattgatggg cttcgaaagc cagacgccat
    36541 ttaccaatga cacggcgctg gcagccaata tcaccaacga aggcggggcg gaaggtcgct
    36601 atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgagtgctt caggagcggc
    36661 aaatcaacga tcttccggcg cttatcgccg caacacaggc acttccggcc tgccgcttca
    36721 tcatcaatcc caatgacgat cgctttatta atcctgacga gatgtgcagc gaaattcagg
    36781 ctgcgtgtcg ggaaacggcg caaccgatcc cggaaagtga tgctgaactg gcgcgctgta
    36841 ttttcgacag tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgtg
    36901 gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc
    36961 agctgtgtgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcgacgc
    37021 tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc
    37081 gtcaggtcgt cagcactacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa
    37141 ttgcccacta tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac
    37201 cactcaactg gaacaggcct gggagctggc gaaacagcgt ttcacggcag tagggattga
    37261 tgtcgaggag gcactgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg
    37321 cgatgatgtt tccggttttg aaaacccgga aggttcgctg accggtggga ttcaggctac
    37381 tggtaattat ccgggcaaag cgcgtaatgc cagcgagcta cgtgccgatc tggaacaggc
    37441 tatgcggctt attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga
    37501 cacgcccgtc gcgcgcgacc agatcaaacc agagcacttc aaaaactggg ttgaatgggc
    37561 gaaaaccaat cagctcggtc tggattttaa cccctcctgt ttttcgcatc cgctaagcgc
    37621 cgatggcttt acgctttccc atgccgacga cagtattcgc cagttctgga ttgatcactg
    37681 caaggccagc cgccgtgttt cagcctattt tggcgagcaa ctcggcacac cgtcggtgat
    37741 gaacatctgg atcccggatg gcatgaaaga tatcaccgtt gaccgtctcg ccccgcgcca
    37801 gcgtctgctg gcagctctgg atgaggtgat cagcgagaag ctggatccgg cgcaccatat
    37861 cgacgccgtt gagagcaaat tgtttggcat tggcgcggag agttacacgg ttggctccaa
    37921 tgagttttac atggggtatg ccaccagccg ccagacggcg ctgtgcctgg acgccgggca
    37981 tttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtgccgca
    38041 gttgctactg cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga
    38101 tgatgaaacc caggcgattg ccagtgagat tgttcgtcac gatctgtttg accgggtgca
    38161 tatcggcctc gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac
    38221 acgcaatatg aaaaaagccc tgctacgtgc gttactggaa cctaccgctg agctgcgcaa
    38281 gctggaagcg gcgggcgatt acactgcgcg tctggcgcta ctggaagagc agaaatcgtt
    38341 gccgtggcag gcggtatggg aaatgtattg tcagcgtcac gatacgccag cgggtagcga
    38401 atggctggag aacgtgcggg cttatgagaa agaaattttg agtcgccgcg ggtaaacact
    38461 gccggatgcg gcacaagtgc cttatccggc ctac
//
LOCUS       DADSZD010000024        38167 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.024, whole genome shotgun sequence.
ACCESSION   DADSZD010000024 DADSZD010000000
VERSION     DADSZD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.024"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            54..1709
                     /locus_tag="J1Z28_001120"
     CDS             54..1709
                     /locus_tag="J1Z28_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HBA7715975.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNTKYAGSSPARTSYGDCMVEMNDIFA
                     NLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHGRTPFRGAKGSTWEGGVRVPTFVYWK
                     GMIQPRKSDGIVDLADLFPTALDLAGHPGAKVANLVPKTTFIDGVDQTSFFLGTNGQS
                     NRKAEHYFLNGKLSAVRMDEFKYHILIQQPYAYTQSGYQGGFTGTVMQTAGSSVFNLY
                     TDPQESDSIGVRHIPMGVPLQTEMHAYMEILKKYPPRAQIKSD"
     gene            complement(1800..3035)
                     /locus_tag="J1Z28_001121"
     CDS             complement(1800..3035)
                     /locus_tag="J1Z28_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBA7715976.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALSLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGKLTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMVDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAYLQVVNK"
     gene            complement(3182..3258)
                     /locus_tag="J1Z28_001122"
     tRNA            complement(3182..3258)
                     /locus_tag="J1Z28_001122"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3222..3224),aa:Pro,seq:tgg)
     gene            complement(3301..3387)
                     /locus_tag="J1Z28_001123"
     tRNA            complement(3301..3387)
                     /locus_tag="J1Z28_001123"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3351..3353),aa:Leu,seq:cag)
     gene            complement(3408..3483)
                     /locus_tag="J1Z28_001124"
     tRNA            complement(3408..3483)
                     /locus_tag="J1Z28_001124"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3448..3450),aa:His,seq:gtg)
     gene            complement(3542..3618)
                     /locus_tag="J1Z28_001125"
     tRNA            complement(3542..3618)
                     /locus_tag="J1Z28_001125"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3582..3584),aa:Arg,seq:ccg)
     gene            complement(3721..5106)
                     /locus_tag="J1Z28_001126"
     CDS             complement(3721..5106)
                     /locus_tag="J1Z28_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7715977.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(5297..6037)
                     /gene="rffM"
                     /locus_tag="J1Z28_001127"
     CDS             complement(5297..6037)
                     /gene="rffM"
                     /locus_tag="J1Z28_001127"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBA7715978.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(6040..7392)
                     /gene="wzyE"
                     /locus_tag="J1Z28_001128"
     CDS             complement(6040..7392)
                     /gene="wzyE"
                     /locus_tag="J1Z28_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen assembly polymerase"
                     /protein_id="HBA7715979.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(7389..8468)
                     /gene="rffT"
                     /locus_tag="J1Z28_001129"
                     /gene_synonym="wecF"
     CDS             complement(7389..8468)
                     /gene="rffT"
                     /locus_tag="J1Z28_001129"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBA7715980.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCLALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFYWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(8465..9715)
                     /gene="wzxE"
                     /locus_tag="J1Z28_001130"
     CDS             complement(8465..9715)
                     /gene="wzxE"
                     /locus_tag="J1Z28_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBA7715981.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(9717..10847)
                     /gene="rffA"
                     /locus_tag="J1Z28_001131"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(9717..10847)
                     /gene="rffA"
                     /locus_tag="J1Z28_001131"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBA7715982.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(10852..11526)
                     /locus_tag="J1Z28_001132"
     CDS             complement(10852..11526)
                     /locus_tag="J1Z28_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-fucosamine acetyltransferase"
                     /protein_id="HBA7715983.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(11504..12385)
                     /gene="rfbA"
                     /locus_tag="J1Z28_001133"
     CDS             complement(11504..12385)
                     /gene="rfbA"
                     /locus_tag="J1Z28_001133"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBA7715984.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(12404..13471)
                     /gene="rffG"
                     /locus_tag="J1Z28_001134"
     CDS             complement(12404..13471)
                     /gene="rffG"
                     /locus_tag="J1Z28_001134"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBA7715985.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHSVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     EIWWKQVQDGSYQGERLGLKG"
     gene            complement(13471..14733)
                     /gene="wecC"
                     /locus_tag="J1Z28_001135"
     CDS             complement(13471..14733)
                     /gene="wecC"
                     /locus_tag="J1Z28_001135"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBA7715986.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(14730..15860)
                     /gene="wecB"
                     /locus_tag="J1Z28_001136"
     CDS             complement(14730..15860)
                     /gene="wecB"
                     /locus_tag="J1Z28_001136"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBA7715987.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(15916..16962)
                     /gene="wzzE"
                     /locus_tag="J1Z28_001137"
     CDS             complement(15916..16962)
                     /gene="wzzE"
                     /locus_tag="J1Z28_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBA7715988.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(16974..18077)
                     /gene="wecA"
                     /locus_tag="J1Z28_001138"
     CDS             complement(16974..18077)
                     /gene="wecA"
                     /locus_tag="J1Z28_001138"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBA7715989.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(18317..19576)
                     /gene="rho"
                     /locus_tag="J1Z28_001139"
     CDS             complement(18317..19576)
                     /gene="rho"
                     /locus_tag="J1Z28_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBA7715990.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(19661..19762)
                     /gene="rhoL"
                     /locus_tag="J1Z28_001140"
     CDS             complement(19661..19762)
                     /gene="rhoL"
                     /locus_tag="J1Z28_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide rhoL"
                     /protein_id="HBA7715991.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(19903..20232)
                     /gene="trxA"
                     /locus_tag="J1Z28_001141"
     CDS             complement(19903..20232)
                     /gene="trxA"
                     /locus_tag="J1Z28_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBA7715992.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            20363..21628
                     /gene="rhlB"
                     /locus_tag="J1Z28_001142"
     CDS             20363..21628
                     /gene="rhlB"
                     /locus_tag="J1Z28_001142"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBA7715993.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            21764..23248
                     /gene="gppA"
                     /locus_tag="J1Z28_001143"
     CDS             21764..23248
                     /gene="gppA"
                     /locus_tag="J1Z28_001143"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBA7715994.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(23295..25316)
                     /gene="rep"
                     /locus_tag="J1Z28_001144"
     CDS             complement(23295..25316)
                     /gene="rep"
                     /locus_tag="J1Z28_001144"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBA7715995.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMLKGQSHLANLKAMMAAKRGK"
     gene            25403..25684
                     /gene="ppiC"
                     /locus_tag="J1Z28_001145"
     CDS             25403..25684
                     /gene="ppiC"
                     /locus_tag="J1Z28_001145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBA7715996.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25730..27205)
                     /gene="ilvC"
                     /locus_tag="J1Z28_001146"
     CDS             complement(25730..27205)
                     /gene="ilvC"
                     /locus_tag="J1Z28_001146"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBA7715997.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            27355..28245
                     /gene="ilvY"
                     /locus_tag="J1Z28_001147"
     CDS             27355..28245
                     /gene="ilvY"
                     /locus_tag="J1Z28_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBA7715998.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSAEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(28242..29786)
                     /gene="ilvA"
                     /locus_tag="J1Z28_001148"
     CDS             complement(28242..29786)
                     /gene="ilvA"
                     /locus_tag="J1Z28_001148"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBA7715999.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29789..31639)
                     /gene="ilvD"
                     /locus_tag="J1Z28_001149"
     CDS             complement(29789..31639)
                     /gene="ilvD"
                     /locus_tag="J1Z28_001149"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBA7716000.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDIIVTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31704..32633)
                     /gene="ilvE"
                     /locus_tag="J1Z28_001150"
     CDS             complement(31704..32633)
                     /gene="ilvE"
                     /locus_tag="J1Z28_001150"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBA7716001.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32653..32916)
                     /gene="ilvM"
                     /locus_tag="J1Z28_001151"
     CDS             complement(32653..32916)
                     /gene="ilvM"
                     /locus_tag="J1Z28_001151"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBA7716002.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32913..34559)
                     /locus_tag="J1Z28_001152"
     CDS             complement(32913..34559)
                     /locus_tag="J1Z28_001152"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBA7716003.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNIDDWQQHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCIVTTDVGQHQMWAAQHIVHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGILGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(34562..34612)
                     /locus_tag="J1Z28_001153"
     CDS             complement(34562..34612)
                     /locus_tag="J1Z28_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716004.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34699..34797)
                     /gene="ilvL"
                     /locus_tag="J1Z28_001154"
     CDS             complement(34699..34797)
                     /gene="ilvL"
                     /locus_tag="J1Z28_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBA7716005.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            35150..36670
                     /locus_tag="J1Z28_001155"
     CDS             35150..36670
                     /locus_tag="J1Z28_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBA7716006.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36695..37033)
                     /gene="maoP"
                     /locus_tag="J1Z28_001156"
     CDS             complement(36695..37033)
                     /gene="maoP"
                     /locus_tag="J1Z28_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBA7716007.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            37152..37991
                     /gene="hdfR"
                     /locus_tag="J1Z28_001157"
     CDS             37152..37991
                     /gene="hdfR"
                     /locus_tag="J1Z28_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBA7716008.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IATLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(38086..38161)
                     /locus_tag="J1Z28_001158"
     tRNA            complement(38086..38161)
                     /locus_tag="J1Z28_001158"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38126..38128),aa:Trp,seq:cca)
BASE COUNT         9332 a        10431 c         9635 g         8769 t
ORIGIN      
        1 atcaatgctt aattacgtca tcattttgac gacatgaaac cggagcattt tgcatggaat
       61 tttcgttttc acccaaactt cttgttgttg ctgtcgccgc agctcttcct ctcatagcca
      121 gcgcagcaga taccccgtca accgccaccg cacgcaaagg ctttgccgga tacgatcacc
      181 caaaccagta tctggttaaa ccggcgacca ctattgccga caacatgatg ccggtgatgc
      241 agcatcctgc acaagacaaa gaaacccagc agaaactggc ggaacttgag aaaaaaaccg
      301 gtaagaaacc gaacgtggtt gttttcttgc tggatgatgt gggctggatg gacgttggct
      361 ttaacggcgg cggcgtggcg gtgggtaacc ctacaccaga tatcgacgcc gttgccagcc
      421 aggggctgat tttaacttcg gcgtattctc aaccgagttc ttccccaacc cgcgccacga
      481 ttctcaccgg acaatactcc atccaccacg gcattctgat gccgccaatg tacgggcaac
      541 cgggcggtct gcaagggtta accacgctgc cgcagttgct gcacgatcag ggctacgtca
      601 cccaggctat cgggaaatgg catatggggg aaaacaaaga gtcgcagccg cagaacgttg
      661 gctttgatga tttccgcggc tttaactcgg tgtccgatat gtataccgaa tggcgtgatg
      721 ttcatgtcaa cccggaagtc gccctgagcc cggatcgttc tgaatacatc caaaaactgc
      781 cgtttagcaa agatgacgtt catgccgtgc gcggcggtga acaggaagcc attgccgaca
      841 ttacgccgaa atatatggaa gatctggatc aacgctggat ggaatatggc gttaagttcc
      901 tcgacaagat ggcgaagagc gataagcctt tcttcctcta ttacggcact cgcggctgtc
      961 acttcgataa ctacccgaac accaaatatg cgggcagctc tccggcgcgc acctcttacg
     1021 gcgactgcat ggtggagatg aacgatatct tcgccaatct gtataaagca ctggagaaaa
     1081 acggtcagct tgataacacg ctgattgtgt tcacctctga taacggtccg gaagccgaag
     1141 ttccgccgca cggtcgcacg ccgttccgcg gtgcgaaagg ttctacatgg gaaggcggcg
     1201 ttcgtgtgcc aaccttcgtt tactggaaag gcatgatcca accgcgtaaa tctgacggca
     1261 ttgtcgatct ggcggatctc ttccctacgg cgctggatct ggcggggcat cctggggcga
     1321 aagtagcgaa tttagtaccg aaaaccacct ttatcgatgg tgtggaccag acatctttct
     1381 tcctgggaac aaatggtcag tctaaccgta aggccgagca ctacttcctc aacggcaaac
     1441 tttctgctgt gcgtatggat gagttcaagt atcacatcct gattcagcaa ccttacgctt
     1501 atacccagag cggatatcag ggtggattca ccggcacagt aatgcaaacg gcgggatcgt
     1561 cggtgtttaa cctgtacacc gatccgcagg aaagcgactc tatcggcgtg cgccatatcc
     1621 caatgggtgt accgctacag accgaaatgc atgcgtatat ggagatcctg aaaaaatatc
     1681 caccacgcgc gcagattaaa tctgattaag ccggcgttat cgctgagatc tacctttgcc
     1741 ggatgcgatg ctgacgcatc cgacaatcaa ccgcaggcgg ccacaaaatg gctgcttttt
     1801 tacttattca ccacctgcaa atacgcctgc ataatgtcgc tggccggacg accgtgcgcc
     1861 agcaaatcag ccattgcttt aagatatggc ggtagatggc ggaaataacg ctgatacccg
     1921 gcacacaaat aattcagccc gggtttgccg ctggcatcga gcataaagcg gtgtttcgga
     1981 cagcctcccc agcacgcttt taacacgtta caactgcgac actgcgccgg taactgctta
     2041 aatttatctt caccaaacac ctgctgttgc ggggaatcta ccatttctgc aattgtctgc
     2101 tgaagcatat ttcctagtcg atactgcgga taaacatagt gatcgcaggc ataaacgtca
     2161 ccgttgtgct caacaatcac cgagcgccca caggttggct gatgatggca aaccgcgccc
     2221 ggtgcgccga caaaattggc gaacgcccat tcgatattca ttacgaaaat cttgccgacg
     2281 tcgcgcttaa tccagtggtc gaaaatcgcc accagaaact caccgaactc ttgcgggcac
     2341 accgaccatt ccgtcagttt gccctgaata tcgccgggag catgtagctt aagtccagca
     2401 tgagcagccg tttcatcagc caggcgctcg accaccggaa taaactggat gaactcaaca
     2461 cctgcatcac acagaaaatc atagacttgc aaaggttgta acgcactggt gcgattgacg
     2521 cagaccagca cgttatagtc gacatgatgt ttttgcagga gcgacagggc acgcatcacc
     2581 agcttatgcg ttggtctgcc acctttggtc acgcgatatt gattgtggat ctcagccgga
     2641 ccatccagcg ataaccctac aagaaaatga ttttctgcca gaaatgcaca ccattcgtca
     2701 tcgagcagca cgccgttagt ctggaagcta ttacttatct tcctgccagc accatatttc
     2761 gcctgtagcg ccacggcacg gcggtaaaaa tccagcccga gtaacgttgg ttcgccgccc
     2821 tgccaggtaa aagccacttc gttttgggtt tcgctggcag cgatatagtg acggacatac
     2881 gcttccagcg tgtcatcgtc catatgcgtg actggctttt cgtggtaaag ggattgtttt
     2941 tcgagataaa aacagtagtc acagttcaga ttacaatcag aaccgctcgg tttcgccatc
     3001 acatgaaaag cacgcgttgg aacctgttgc tgcatggtcg ctccttgcct gaagtcatcc
     3061 ttcaataagt taagggcgaa atcataccgt ggcgcaatgc gttattacgg gcgtttattg
     3121 cgaagggaaa gtgacgaaat gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa
     3181 ttggtcggcg agagaggatt cgaacctccg acccactggt cccaaaccag ttgcgctacc
     3241 aagctgcgct actcgccgaa atactgcttt ttgaattttt agttcaattc tttaaagtcg
     3301 tggtgcgagg ggggggactt gaacccccac gtccgtaagg acactaacac ctgaagctag
     3361 cgcgtctacc aattccgcca ccttcgcatt gtcacaactt ctaataatgg ggtggctaat
     3421 gggattcgaa cccacgacaa ctggaatcac aatccagggc tctaccaact gagctatagc
     3481 caccactaca aatcttgtta cgcggtatta ctaccaccgc agctcaagcg ccgggactaa
     3541 atggcgcgcc cgacaggatt cgaacctgag acctctgcct ccggagggca gcgctctatc
     3601 cagctgagct acgggcgctt agcgccgttg cgggggtgga taatacggac ttcccacccc
     3661 tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc gttttgctgc
     3721 ttattcctcc agtttatgcg ctttaccgtg gcgattaagc ccaaaaactt tataaatcgc
     3781 cgtcaccacc agcataaaga tgatgccgac aaacagcgac atacgcgtat cttcattaaa
     3841 gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtagttgg cccaagggaa
     3901 caggattgag cggaacggat ggctggcaat tgccgctttg tgtgcacggc gaaaacgcag
     3961 ttggcttatc aaaatcacaa accatggcac catacccgga agcacgctgg cgctgtagac
     4021 gtagacaaac acacgctgtg gattgggaat gatgtagttc aggcatgaac caattagcag
     4081 aatagcaata gatactgcca cacccgcaac cggtacgccg tgacgggaaa ctttcgccat
     4141 tgccgccggt aactgacggt ttttcgccag tgcatagagc atacgtccgc aactgtacat
     4201 gccgctgtta cagccagaga gcgcagccgt cagcaccaca aagttgataa tgcccgccgc
     4261 tgcggtaatg ccgattttgg cgaaagtcag tacgaacggg ctaccgttgc tgcctatttc
     4321 attccacggg aagatagtaa cgataacgaa aatcgcgcca acgtagaaaa tcaggattcg
     4381 ccacagcacc ttgcctacgg cactgcgcag cgtcacctgc ggattcttcg cttcaccggc
     4441 agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca atgcggtcag
     4501 gaaccctttc cagccaccag caaagaaacc gccatgctct gtgagattgc taaaaccaat
     4561 cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga caatcatcac
     4621 gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt acaacctcac
     4681 cgcagccaga ttcgccaacg ccaccagcgc caccgcgatc aatgcgggta tccactgcgc
     4741 catctccggg aaccagaact ggacataaac gccaatggcg gtgatttctg agatccccac
     4801 cgccatccac ataaaccagt aagaccaggc ggtgagataa ccaaagaacg ggctcatata
     4861 acgatgcgcg taaacggcga acgaaccggt aaccggttcg aggaacaaca tttcgcccat
     4921 tgaacgcatg atgaaaaaga cgaacagccc ggcgatgata taggccaaca atacggatgg
     4981 cccggcccat ttcagggtac tggcggcccc cataaacagg ccgacgccaa tggtgccccc
     5041 cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg gtttgttatc
     5101 tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta tcgttatggg
     5161 tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa
     5221 atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg aagtgaccac tccgtcgctt
     5281 tacaaagaga ggaaaatcag aggttgccgg tgtaatgcca gcgtaaataa cgcagcaaac
     5341 gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac tccagcccca
     5401 gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg taggtcccgc
     5461 caacgcccat atacagcgca tctggatgca ccagacggca gtcgcgcatg aagatctcct
     5521 gctttggcga tcccatcgca acggtgacga tttgcgctcc gctggcatga atgcgttcaa
     5581 acagcgcctg acgctgctcg ggtttaaaat aaccatcctg actgccaacg atattcacat
     5641 tccactggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta ccgcccacaa
     5701 gaaataccgg tgtcccttct ttgcctgcgc gcgccatcag ctcttcccag agatcggccc
     5761 cagcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt acaacgctga
     5821 tgccatccgc atatttaaat tcggcagcgt taattaactc cctgacctcg gcgttatctt
     5881 caatggtcag cattttttcg gcattaatgg caaccagcgt tccctgctta agctgcccgt
     5941 cagcgaacag ataatcgagg gcatgctgca tatcacgcca accaatcaac tgtaagccac
     6001 gcagcgtata ggtcggtgcc gtggtgttgt tattcattgt tatccttcaa cctgcgtccg
     6061 gagcgatgat tttgttcgtt tatgaatgag tccggcgctt tcaaaaagcc agtacaacag
     6121 ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagaccacgc gtgagacaaa
     6181 cgaatccagc ccttcacgcg ccagcacgat catattgaag atcgccccga agcagaaact
     6241 gtgcaatatc gcagccttat agcgattagt ctcgcggttg cccagctcat acagccagtc
     6301 gaaccatttg ataatcagtc caaccacaat cgccccgagc gggatgaaca acgcgccgcc
     6361 cataacaacc agtgagccta taagcgtagg cgagatcgcc agtccggagt ggttattcag
     6421 cacttcccag gtaaagtagt tggcggagtt cagcaccata ctcgggcgac ccggccacag
     6481 ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct ggaagtcgat
     6541 gttgtcgtag ttctgcaaca gcaacgccag attctcccac ggcgagaagg tgtcgcgagt
     6601 gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat agcgttttag
     6661 cgccagccag aacatgccca caatccccag cacgcccgcc gccgccagca tccacaacga
     6721 aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga tgatattggc
     6781 gcgagtgccg ccgacaatca tgtaagtcaa caagccaaag gcaaccgtgc tgacgaggaa
     6841 aaacagccac gctttgctat cctggcgcag aaaataaacc accagcatcg ccgggatgaa
     6901 aaagtaaaag aagcgtttta atgccacgcc ggagacttcg ctggaaaaga tctgactgta
     6961 agaattaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga cgctcaccag
     7021 agcgatgccc atcaggatca cccacgtgag attggtctcc acgcggttca tggtaaacag
     7081 cggacggcgc ggtgcatcag caacgcgttt gcgtagacgg gttttgtagg tgacatagta
     7141 aaccgcgtag aagcagcctg cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac
     7201 accaacatca aagcgaaata ccagcacgct ggtcagcgga aagccgaaga aaaaggtgag
     7261 caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa actcaaacca
     7321 ggtcagcgtg gcaataaaca gcgtgcagag cagccagaca acaaacaggc cactgaattg
     7381 cagcagactc atgcgacctc cccggcggca atcgccaacg cccgctgcca gccttgtaga
     7441 tagttagggc taaagaaggc aatggtgttt ttatccaccg acgccaactg gcgctgcgct
     7501 tcacgcacaa tatcctcgtt gagatcatcg gtagtaaaca gtactggcaa atgctgttcc
     7561 gtcatatcct gccagaacgg attttcccgg ttaagcacgc aaggaatgcc cgcctgaatc
     7621 agtaagcaaa gcgtaccaat gccctgctgg cgggcaaaaa taaagtaacc gagatcgcac
     7681 tggcgaagta gcgtcagata ggcgtcaaat tccagttttt cgctcagaac ttgtaggttt
     7741 tcttcgctga ataactccag tcccgcctga cgaacttcct cgatgtacgc ttcgttatta
     7801 ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg atgaacggcg
     7861 cgcaaggcag caatatgctc attgctgcgg tcaccggagt tccccaccag aatggtcatt
     7921 tttccttcac gttgccgatc gttcgccatc gtattaagag aagggtccat ccgcgtcggg
     7981 aagtacagca gttctccccg cacctttgga tgcgttttgg caaaaaagct caaatcaccg
     8041 cgggtggcaa atacacagcc gactctcttt tgcgccaggc gacgtagcgg gtaaaaaagc
     8101 ttatatctca agccactgga aagctcatac aggtctgctc cccagatatg ccagtaaaac
     8161 tggctgggct taatgccacc actcagcaga gccagccaca gtgtgggatt gaactgaccg
     8221 tggaagaaaa aacgctgctg acggttagct tttgctttcg cgatgaccgc ttccgccagc
     8281 gattttttcc cagggaaaaa ttgcacagaa agcgccagac agctatcact taagccgtcg
     8341 tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcggccag cgcgtcgttg
     8401 aaaaaccgca gaacggttcg gttatggtga gggatatccg atcccagtac gtgaatcagt
     8461 acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa aaatagacga
     8521 tataggttgc catataggcc tgtgctgcgc ccagtgcgcc atgcgcaggg attagccagt
     8581 gggcaaatac catcaataaa gtgaactggc tgatttccgc cagaatataa aaccgcagtg
     8641 acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac acatcaccca
     8701 ccaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac agcagccaga
     8761 tagcaaaatc acgcagcaac cagacggtaa aactcgccgc cgccactgcc ggtaagacga
     8821 atttcagcga tttaaccact tcccgggtga tatcgcgctt ttccgttaac cgcgacaacg
     8881 tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca tcggaaatac
     8941 tgctcacccc ttgccagatc cccacctcat cccagctata ctgcgccgcc agcagtttac
     9001 gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt
     9061 tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt aacgggatga
     9121 caccacgttt gatcaacatg atggcggcag gaattaccac cagcgcggga atcagcgcca
     9181 gacccagcaa cgcaccttca taaccgccca aacgataact gacgtagtac gcgagaacgc
     9241 caatcaagct accgacaatc agagataacg cattacctgc ggcatcgcga aagcctttca
     9301 tcagcgccag taacaggttg ccccaggcga tccccatttg caccagcgcc actaaacgca
     9361 ccagcccctg atagtcggta ttaccaaaca acccttggct gattggcgca gctgccagca
     9421 caaaaaccag cgccattagc gtagagaagc caagtaccat cgctgatgaa gtgccgacca
     9481 cgcggcgcag ctgttgggga ttatcatgat actgggcaac gtatttggtt acgccgttaa
     9541 agatgcctgc cccggcaagc acgccgagca cggtaatcaa ctggcggaaa tttgccgcca
     9601 gcccaagccc cgccggacca aatgacactg ccagcaactt accgaccagt aacccggcac
     9661 caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac gacatatcag
     9721 gagaagtagt tcaacaaggt cgcaattacc gtacgctgat tgacgggcga caggttgtag
     9781 aacaacggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg
     9841 aactcaccaa agcgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc
     9901 atgatttccg cttctttcag aaagttaatc aatgcgctcc ggtcatcaat atcccgcagt
     9961 ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg cagctcgata
    10021 cgcccggctt tcgccagagg tgctaacgca tcgtagtagt tttgccacag cgccagacgc
    10081 tgctgattga tacgatccgc tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg
    10141 gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg accacggaag
    10201 aactggctgc ggtttgtgcc tttttcacgg atgatctctg ctcgttcgat caacgcttta
    10261 tcgttaatca gtgtcgcgcc gccttcacca cccgccgtgt agtttttggt ttcatggaag
    10321 ctaaagcagc caatatgacc aatggttccc agcgcacgcc ctttgtaagt ggacatcacg
    10381 ccctgagcgg catcttccac cacaaacaga ttatgcttct tcgccaacgc cataatggtg
    10441 tccatttcgc aggccacacc cgcgtaatgg accggcacga taacgcgcgt tttgtcggtg
    10501 atcgccgctt caatcagcgt ttcgtcgatg ttcatagtgt ccgggcgaac atccacaaag
    10561 acgattttcg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt gtagctcggc
    10621 atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc cagcgaagcg
    10681 gtgcaggatg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag ccactgctgg
    10741 cagcgacggg taaaaccacc atcgccgcac agtttgccgc tacccattgc cgactgcata
    10801 tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg atcacctgta
    10861 taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt taagcgcggc
    10921 ggtgttgccc atctgggtcg ccacccgcaa agttgtttta ccgcgagcat acgcccagtt
    10981 tagcgccgtt tgcattagct cagcacctgc accgcgtcca gccagcaggc caattcgcgc
    11041 atctgtcgca ttgagttccc gtaaagagac ataaccgcga atatcgccgg acgccgcacg
    11101 taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc actgtgcata
    11161 aaagcgaccg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc
    11221 ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta ctgcaccgct
    11281 atcactgaca ttgttcacgg gtagcgccaa atcaacttca ccttctacca gggagaatcc
    11341 cagctgttgc agggcatcca gttcacccgt atttgatgcc gcaattttgg cctgcacccg
    11401 tgaccacggc gctaacgcgt ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat
    11461 ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac tggcgcggac
    11521 gggcacgaag taactccagc agatattgac cgtagccagt tttcgctaat gaactggcag
    11581 cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcaatctct tccaggcagg
    11641 caatcttaaa gccctggcgt ttttctactg tctgcacaaa agtgctggcc tcaatcaggc
    11701 tgtcgtgagt gccggtatcc agccaggcaa atccgcgccc gagcagttca acggtcaggt
    11761 tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcacca cgctccgacg
    11821 gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc ccggtcaccg
    11881 cccagtttga tttcggctgt tttggttttt cttccagcga gatagcgcgg aaattgtcgt
    11941 caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaat accgtcgccc
    12001 cttccgtgcg cgccgcaaca tgacgcagct tcggactgaa gccctgtccg aagaagatgt
    12061 tatcgcccag caccagacaa gaaggttcac cgttgaggaa ggtttcaccg atgataaagg
    12121 cctgcgccag accgtccgga ctgggctgtt cggcatattc cagctggata ccgaactcac
    12181 tgccgtcacc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg atgataagaa
    12241 tttcgcggat accggccagc atcagcaccg acagcggata gtaaatcatt ggcttatcgt
    12301 aaatcggcaa cagttgcttc gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac
    12361 cgcccgccag gataatacct ttcatgtctg cctccgctga aaattagcct ttcagaccta
    12421 aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagatttca ttagccagat
    12481 accactgcac cgttttacgc attccacttt caaaggtttc ctgcggcagc cagccaagtt
    12541 cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga cggtcagcga
    12601 caaaggtgat caggtcacga tagtgcgcca cgctgtgcgg cttattcgga gccagttctt
    12661 ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg tgaccaccaa
    12721 tattataggt ttcaccgact ttccctgtgg tcgccacgca atacagcgcg cgggcgtgat
    12781 cttcaacata cagccagtca cggatttgct gcccgttgcc atataccggc agcggtttac
    12841 ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg taaggaccgt
    12901 agttattcga gcagttggtg ataagcgtcg gcaaaccata ggtccgcagc caggcgcgca
    12961 ccagatggtc gctgctggct tttgacgcgg aataagggct gctcggcgca tacggcgtgg
    13021 tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg gtggagatat
    13081 gatgaaaacg gaacgctgat tttttatctt ccgtcagcgc agtccagtaa gcccgcgccg
    13141 cttcaagcag tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa
    13201 tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc tgatactcag
    13261 tgaatacgcg cgccagttct gcccgatcgc agatatcaac tttctcaaag gcaaagcgct
    13321 cgctttgcgc gaccggtgcc agagacatca ggtttccggc gtaggtcagc ttatcgacca
    13381 ccaccaccgc gtcgctcgtt tcgttgatga tataacgcac cagcgccgag ccaataaacc
    13441 cggcaccacc tgttatcaga atctttctca tcagcgccag actcctttgg catcgacgac
    13501 atactgctga tggacgttgt cgccattgat aactttgaac tgactatgat cgaccagcat
    13561 caccagcaca tctgccgttg ccagcgcctc gtcaagctgc gccagagtac aaagcccggt
    13621 cagtttcttc ggcaactggt ggatgttagg ctcaacaacc agcgtttcgc cgctgtgcca
    13681 ctgggcgatc agttcagcga tttccatcgc agggctttca cgcaggtcat caatattcgg
    13741 tttaaacgcc agaccaaagc aggcgatttt cagttcactg gcgcgtttat cggtagcagc
    13801 cagacaatca gccaccgccg ctttcacctg atcgataacc cagaacggtt tgtgatcgtt
    13861 cacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca cgataaacca
    13921 cggatcaaca gcgatgcagt gaccgcccac gccagggcct ggctgaagaa tattgacgcg
    13981 agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat cggcacaaat
    14041 cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt cggtgagctt
    14101 acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga aaattttgta
    14161 cagttcgctg gcgcgggccg aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt
    14221 aatcagctcg accataacct ggcccggtaa tacgcgttcc gggcagtaag caatgttgac
    14281 gtccgccaac tcgcccacct gctgtgggaa agtgagatct ggacgcatct ctgctaacca
    14341 ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca ccaacgcgcc
    14401 ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca tatctggctc
    14461 atgatcgcct ttaaacggcg tgggtacagc aatcaaccag gcatccgctt caactggcgt
    14521 cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacgctcg ccaaatcagg
    14581 ctcgacgata tggatttcgc cacgattgat ggtatcaacc gcgtgttggt tgatatcgac
    14641 accaatcacc tgtttttgcc ttgaggcaaa cgcagctgct gtaggcagcc cgatgtaacc
    14701 cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt ttttaacgct
    14761 tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg gctcatagct
    14821 tgatattcgt tttcgtcttt taaaagacgc gtcacttcct cgacaattcg ctgcttatcc
    14881 gtgccgacca gacgcaccgt ccctgctgtc accgcttccg gacgctcagt ggtatcgcgc
    14941 atcaccagca ccggtttccc cagcgaaggc gcttcttcct gaatgccgcc tgagtcagtc
    15001 aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg gggatcgatc
    15061 agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac gttcggattg
    15121 agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgca
    15181 tggcagattt cttcaaagcc acgaccgaag ctctcacgcc tgtgaccggt caccagaatc
    15241 atctttttat cggggtcgat aaacgggtaa tttgccgcca gttctgaacg cagcgtatcg
    15301 ctgctcatca cctggtcacg cacccataac agtgcatcaa tgactgtatt accagtaatg
    15361 aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt ttcggttgga
    15421 gagaagtgat acatcgccag atgcccggtc aatgtacggt tagcctcttc cggccacggc
    15481 gaatagagat cgcccgtgcg cagaccagcc tcaacgtgac caacaggaat acgctgataa
    15541 aacgccgcca ggctggttgc cagcgtcgtc gtagtatcgc cgtgaaccag cacgacgtct
    15601 ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt tatctctgta
    15661 aggccctgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga aaagagtttc
    15721 agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa
    15781 ggatcttttg ccaacgcatg caccaacggt gccatcttga tggcttccgg gcgcgtacca
    15841 aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc ctttgcgctc
    15901 accgcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag ccccgatcag
    15961 ccccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat cgcgttttac
    16021 cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca gggttggacc
    16081 aacattcagg gtgtttaaca tggcccgatt ttgatcatag tcgagatcaa aggccggacc
    16141 gacggcctgt aaattttcca gtcgagcctg gagcattgga cgcccaagca ggaacatttc
    16201 tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat tatgctgctc
    16261 agcaattttc agcgcctgtt caatactgtt catccggcga tcgtagatag ctttcgccac
    16321 ctcttcctga cgcttcacct gagctttcat ctggatggtg cgtgccgccc atgcgccttt
    16381 cagctcatca ttcagatggc tggctgcacg ctggctggca aaagcaacat actgacgtaa
    16441 caggttatta gcgtcaggcg cagtttcggc aatcagcttc acgctgtcgt tgaccgcgcg
    16501 ggtgaagtca ccgggaataa acacgatgtt gttgatcatt tcatccagca acgccgcatc
    16561 ggctttgcta ttgcccacca tccgctgttt gtaatagtcg gtttgcagcc agaactctct
    16621 gcgggtatcc cacgaggcca gttgcataac aaactcttta taggcttcgt ccatgaccga
    16681 tggttggtcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa attgctgctg
    16741 tgagtaatat ccccccagca tattcaccgt tggacgatcg gtaatcgctg tcgagctcca
    16801 ctcctggcga gcaaaaaaag tatacgccag cgcgattaac gcaaacgcca gccccatgcc
    16861 aataatccac agcttcccag cccacaaggt acgaaacaac ccacgaatat ccagttcatt
    16921 ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct catttatttg
    16981 gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg cttaataaag
    17041 cgagcaactt tccaggcacg cttgatgcaa tatccataga ggaagaatgc tagcaaaaag
    17101 agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac gccaatggaa
    17161 gcgagcagtg ctgcggcaag ggtaatcagc acaaacgcct ggcgggaagt aaacccggca
    17221 cgcatgatca aatggtgaat atgctgacgg tctggagaga atgggctcat gcctttacgc
    17281 aggcgacggt acataatcgc caccatatcc attagcggaa tggcgattat ccacaaagcg
    17341 gtaaccgggc tgatgggatg agttttgccc tgggtcgttt cgagcaggat ccagataacg
    17401 gtaaaaccaa tcagcgtact gcccgcatcc cccataaaga ctttgtagcg gcgacccagg
    17461 ataccaaggt taagcatgat gtatggcagg atggcagcga tcatcgcaaa gcaccagatt
    17521 gcgagactgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa cgagacgcag
    17581 gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc
    17641 cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc ccaggagcca
    17701 aagatataac ccaggctact gagataaagt ttgccgaaca ccatcataac aatgccaaca
    17761 gcagcctgta tggtggcacg gatttttacg ctgatatcaa aacggtcatc cagcgcgcca
    17821 atgaaaacaa gcacaccagc acaagcgaga tagagagatg catgcggaat atagtaatca
    17881 acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac gagaggtatc
    17941 aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt ttttgccacc
    18001 ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag atcagtactc
    18061 actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa gtataaccac
    18121 gaagaccttt attcagaagg gaaagtccgt tccgaaccca ccagccctgt gcaaaaatta
    18181 accatttgat atgcaaattg cataacacac cagcaaattc caggaatttt cctagcatcg
    18241 ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt
    18301 ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca ttggtcttgg
    18361 tcattgccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc gggtgaatga
    18421 ttttgcgcag gatccacatt ttctgcagtt cttcctgagt cgtgagcagc tcttctttac
    18481 gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg atcttacgag
    18541 agagatgcag ttccatgttg cctgtacctt taaactcttc gtagataact tcgtccattt
    18601 tagaaccggt atcgataagc gccgtcgcga taatggttag gctgccgccc tcttccacgt
    18661 tacgcgccgc accgaagaag cgtttcggac gatgcagggc gttggcgtcc acaccaccgg
    18721 tcaacacttt acctgacgcc ggaacaacgg tgttgtaagc gcgcgccaga cgagtgatgg
    18781 agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttcgcc ttctcgatca
    18841 ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt
    18901 caccttttac cagtcgctgc atctcggtta cttcttccgg acgttcgtcg atcagcagaa
    18961 ccatcagcac acaatccggg tggttgtaag caatgctctg agcaatgttc tgcagcagca
    19021 tggttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga ccgataggtg
    19081 atgccagatc cagtacgcga gcggttaaat cttccgtaga accgttacca cgttccatac
    19141 gcagacgaga gtttgcgtgc agcggggtta agttctcaaa gaggattttg ttgcgggcgt
    19201 tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag cgttcacctt
    19261 ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg aaacggcgga
    19321 tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg tctgcggaac
    19381 ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag atatcttcgc
    19441 cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc atacgagcca
    19501 ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc gtattcttta
    19561 attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg
    19621 ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc atgtcttttc
    19681 gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg agtgacgagc
    19741 cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat tacaagattc
    19801 aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac tcacgccggg
    19861 cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca ggttagcgtc
    19921 gaggaactct ttcaactgac ctttagacag tgcgcccact ttggttgccg ccacttcacc
    19981 gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg cagtgccagg
    20041 gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt cagcgatttc
    20101 atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga aatcgacgag
    20161 gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat
    20221 tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc gttgttggtg
    20281 taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt aaagcaatag
    20341 taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga agttttccga
    20401 cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac
    20461 gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc
    20521 gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt atcttctctc
    20581 tcatcccgcg attgccgatc gcaaggtgaa tcagccgcgt gccttaatta tggcaccgac
    20641 gcgtgaactt gccgtgcaga tccacgccga cgcggaaccg ctggcggaag ctaccggcct
    20701 gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag
    20761 cggcgttgac attctgattg gcaccacggg tcgtttaatt gactacgcca agcagaacca
    20821 cattaacctc ggtgccattc aggtggtggt actggacgaa gccgatcgca tgtacgatct
    20881 gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct
    20941 caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt tcgagcagat
    21001 gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc gtattaaaga
    21061 agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga
    21121 agagtggcca gacagagcga ttattttcgc caacaccaaa caccgttgtg aagagatctg
    21181 gggccacctg gcggcagatg gtcatcgtgt cggtttattg acaggcgatg tcgcgcagaa
    21241 aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc tggttgccac
    21301 cgacgttgcc gcgcgtggtt tgcatattcc tgctgtgaca cacgtcttta actacgattt
    21361 acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg caggcgcaag
    21421 cggtcactct attagcctgg cgtgtgaaga gtatgcattg aatttgcctg ctattgagac
    21481 ctatattggt cactcaattc cggtaagtaa atacaatccg gacgcattga tgaccgatct
    21541 gccaaaaccg ctgcgcctca cgcgcccgcg cacaggcaat ggtccgcgtc gtactggcgc
    21601 tccgcgtaat cgtcgtcgtt caggttaaag aaaaaatgtc atactccaaa tcattcaagc
    21661 tgtatcaaga tggcaagaga atgaatcccg atgcgcttac tgtcgtaagt gattcaggtg
    21721 aaggaatgca gccaacgcag agacagattg aaggatgaag agtatgggtt ccacctcgtc
    21781 gctgtatgca gccattgatc tcggttcgaa tagttttcat atgctggttg tgcgcgaggt
    21841 ggctggaagc atccagacgc tgacgcgaat aaaacgcaaa gtgcgtctgg ctgccggcct
    21901 gaatagcgaa aatgccctgt ctaatgaagc aatggagcgc ggttggcaat gtctgcgcct
    21961 gtttgctgaa cgtctgcaag atatccctcc ctcgcaaatt cgcgttgtcg ctacggcgac
    22021 attacgactt gccgtcaatg cgggtgattt tattgccaaa gcgcaggaaa tcctcggttg
    22081 tccggtacag gtgatcagcg gtgaagagga agcgcgtctg atttatcagg gcgttgctca
    22141 caccactggc ggtgccgatc agcgcctggt ggtggatatc ggcggagcca gtactgaact
    22201 ggtaaccggc acgggtgcac aaaccacctc tttgttcagc ctgtcgatgg gctgcgttac
    22261 ctggctggaa cgctattttg ccgatcgtaa tctggggcag gaaaattttg atgctgcaga
    22321 aaaagcggca cgcgaagtgt tacgtccggt tgccgatgaa ttacggtatc acggctggaa
    22381 agtgtgcgtt ggcgcttccg gcaccgtgca ggcgttacag gaaatcatga tggcacaggg
    22441 gatggatgaa cgcattaccc tggaaaagtt gcagcaactg aaacaacgcg ccattcattg
    22501 cggtcggctg gaagagctag agattgacgg gctgacgctg gaacgtgcgt tagtgttccc
    22561 gagtggtctg gcgatcctga tagccatttt taccgaactg aatattcagt gtatgaccct
    22621 ggcgggcggt gcgctgcgtg aaggcctggt ctacggtatg ttgcatctta ccgtcgagca
    22681 ggatattcgc agccgtacgc tgcgtaatat tcagcgccgc tttatgatcg atattgatca
    22741 ggcacagcgc gtagccaaag ttgcggctaa cttcttcgat caggtagaaa atgaatggca
    22801 tcttgaagca ataagccgcg atttgctcat cagcgcctgt cagcttcatg aaatcggcct
    22861 gagcgttgac ttcaaacaag cgccgcaaca cgctgcttat ctggtacgta atctggatct
    22921 tcccggtttt acccccgcac agaaaaaact gctggcgacg ctactgctca accagactaa
    22981 tccggtcgat ctctcatcgc tgcatcagca aaatgccgta ccaccgcgcg tcgcagaaca
    23041 actctgccgt ttactccgcc tggctatcat ttttgccagc cgtcgccgtg acgatctcgt
    23101 gccagagatg acattacagg ctaaccatga actgttgacc ttgacgcttc cgcaaggttg
    23161 gctaacccaa catccgctgg gtaaagagat tattgatcag gaaagccagt ggcagagcta
    23221 tgtccactgg ccgctggaag tgcattaatg agtaagtgcc ggatgcgatg ctgacacatc
    23281 ttttccggcc ttgattattt ccctcgtttt gctgccatca tcgctttcag atttgccaaa
    23341 tgactttgcc ctttcagcat ccgttcttcg gcgctgacca ctttgcgctc ctgttcccaa
    23401 atcaaatcat cctgcggcag ctccagcagg aagcggctcg gctccgggcg caccagttcg
    23461 ccgtactgac ggcgttcttt acacagcgta aaggtcaatt ccttctgggc gcgggtaatg
    23521 ccgacatagg ccagccgccg ctcctcatcg atattatctt catcgatact gctctggtgg
    23581 ggcaaaaatc cttcttccat accaaccatg tagacatagg gaaactccag tccttttgac
    23641 gcgtgcaaag tcatcagttg cacctgatcc agctcttcct cgctctcgcc gcgctccatc
    23701 atgtcgcgca gagtaaagcg cgtcaccacc tgggtgagcg tcatcggctc atccagttcg
    23761 ctgccttcca gcatctccgt catccagcta aacagttggt tgacgttctt catgcgcatt
    23821 tcggcagctt tcgggctggg cgatgtttcg tacagccagg attcataatc catgccgtgg
    23881 atcagatcgc gcaccgcggc aatcggctcc cgctccgcca gacgctggat ttctgccagc
    23941 cagtgagtga agcgggtcaa tgcttcataa ccacgtccgc taagcgtctg actcaggccc
    24001 atatcaaagc tggcggtaaa catgctttta ttgcgcgtca tcgcccattc acctagcttt
    24061 ttcagcgtcg ccgggccaat ctctcgcttc ggcgtgttaa cgatacgcag aaatgcgctg
    24121 tcatcatccg ggttagtcag cacgcgcaga taagccagca aatccttgat ttcaggacga
    24181 gagaaaaacg acgtaccacc agaaattttg tacgggatgc ggttttgcat caggaatttt
    24241 tcgaataccc gcgactgatg gttgccacga taaagaatgg cgtaatcttt gtactgcgtt
    24301 ttattgacga agtgatgggc gatcagctcg ccggtgacgc gctcggcttc atgttcctcg
    24361 ttattcgcgc ttaaaacttt tagctctgtg ccgtaaccca gttcggagaa cagacgcttt
    24421 tcaaagacgt gcgggttatt ggcgatcagg atgttcgccg ctttcaaaat acgtccggaa
    24481 gagcggtagt tctgctcaag cttaatcacc ttcagcgccg gaaaatcctg gctcagcagc
    24541 accagattct gcggacgtgc gccgcgccag gagtagatcg actggtcgtc atcgcccacc
    24601 acggtaaagc gcgcgcggct gcccaccagc agtttcacca gttcatactg gctggtgttg
    24661 gtgtcctgat actcatccac cagcaggtag cgaattttgt tctgccagcg ctcgcggact
    24721 tcttcattgc gttgcagcag caacgttggc aataaaatca gatcatcgaa atcgagaacg
    24781 ttacaggctt tcaggtgtgc atcatacagc ccataacaat gggcaaaaat acggtcccgc
    24841 tcgccctttg cctctgccgc cgcctgcgcc ggtgttttga gatcgttctt ccagttggag
    24901 atggtcgaaa ttagctgttg caggagaact ttgtcatctt caatcagccc ctcggtcaac
    24961 tctttaagca aagcaagctg atcggtatcg tcaaacagcg aaaagttagc cttcatcccc
    25021 agcgccgcat actcgcgttt gatgatatcc agccccagcg tgtggaaggt ggagatcatc
    25081 agcccacgcg cctctttgcg ccccagcgtc tgcccgacac gctctttcat ctcgcgcgcc
    25141 gccttattgg taaaggtcac cgccgcgata tgtcgcgcct gataaccgca accgcggatc
    25201 agatgggcga ttttattggt gataacacga gttttaccag aacccgcgcc cgccagcacc
    25261 aggcaggggc cggtaacgaa ttcgacagct tgttgttggc cggggtttag acgcatagga
    25321 aaatcgctca atgaaagtcg ggaggggaaa ataacagcgt ggtagtatag ccagcctcat
    25381 ccttaccact caaggcacga tcatggcaaa aacagcagca gcactgcata tccttgtaaa
    25441 agaagagaaa ctggctctgg atcttctcga gcagatcaag aacggtgccg atttcggcaa
    25501 gctggcgaag aaacactcca tttgcccgtc aggcaaacgc ggcggtgatt tgggtgaatt
    25561 ccgccagggt caaatggttc cggcgttcga taaagtggtt ttctcttgtc cggtactgga
    25621 gccaaccggc ccgctgcata cccagttcgg ttaccacatc attaaagtgc tgtaccgtaa
    25681 ctaacagaaa ggccttctcc gggagaaggc cttgaactct gaatcgctat taacccgcaa
    25741 cagcaatacg tttcatatcc gtcatatagc cgcgcagttt cttacctacc tgctcaatcg
    25801 catggctgcg aatcgcttca tttacatcac gcagctgcgc gttatctacc gcaccttccg
    25861 gaatagcttt acccaggtcg cccggttgca gctctgccat aaacggtttc agcagcggca
    25921 cacaagcgta agagaacaga tagttaccgt actcggcagt atcggagata accacgttca
    25981 tttcgtacag acgcttacgg gcgatggtgt tggcaatcag cggcagttcg tgcagtgatt
    26041 catagtaagc agattcttcg atgatgccgg aatcaaccat ggtttcaaac gccaactcaa
    26101 cgcctgcttt taccatcgcg atcatcagta cgcctttatc gaagtactcc tgttcgccga
    26161 ttttgccttc atactgcggc gcggtttcga atgcggtttt gccagtctct tcacgccagg
    26221 tcagcagttt cttatcgtcg ttggcccagt cagccatcat gccggaggag aattcgccgg
    26281 agataatgtc gtccatatgt ttctggaaca gcggcgccat gatctctttc agttgctcag
    26341 agagcgcgta agcacgcagt ttcgccgggt tagaaagacg gtccatcatc agggtgatgc
    26401 cgccctgttt cagcgcttcg gtgatggttt cccaaccgaa ctgaatcagt ttttctgcgt
    26461 atgccggatc ggtgccttct tccaccagct tgtcgaagca cagcagagaa ccagcttgca
    26521 acataccgca caggatggtt tgctcgccca tcaggtcaga tttcacttcc gcaacgaaag
    26581 aggattccag aacgcccgca cggtgaccgc cggttgcagc tgcccatgct ttagcgatcg
    26641 ccatgccttc gcctttcgga tcgttttccg ggtgaacggc aatcagcgtc ggtacgccga
    26701 atccacgttt gtactcttca cgtacttcgg tgccagggca tttcggcgca accattacga
    26761 cggtgatgtc tttacggatc tgctcaccca cttctacgat attgaaacca tgagagtagc
    26821 ccagcgccgc gccgtctttc atcagtggct gtacggtgcg cactacatca gagtgctgct
    26881 tgtccggcgt caggttaacc accagatccg cctgcgggat cagttcttcg taagtaccca
    26941 ctttaaaacc attttcggtt gctttacgcc aggatgcgcg cttctcagca atcgcttctt
    27001 tacgcagagc gtaggagata tcgagaccag aatcacgcat gttcaggccc tggttcagac
    27061 cctgtgcacc acagccgacg atgactactt ttttaccctg aaggtagctc gcgccatcgg
    27121 cgaattcatc gcgccccata aagcgacatt tgcccagctg tgccaactgc tggcgcagat
    27181 tcagtgtatt gaagtagtta gccatggtga ttcctcgtga tgttgtgctt cttattgttc
    27241 ggttcgcgtt tgcgagattg aattcactat atgacaggaa atttattgcg gaaattgata
    27301 tattcacaac gtcacattgc aatttctgca acgtcaacat taagggctgc gccattggat
    27361 ttacgcgatc tgaaaacctt cctgcatctg gcggaaagcc gccattttgg ccgcagcgcg
    27421 cgggcaatgc acgttagccc gtccacgctc tctcggcaga ttcagcgcct ggaagacgat
    27481 ctcggtcagc cgctgtttgt gcgcgataac cgcacggtaa cgctaaccga agcaggcgaa
    27541 gaactgcgcg ttttcgccca gcaaacgctg ttgcagtatc aacagctgcg ccacaccatc
    27601 gatcagcaag gaccgtcgct ctctggcgaa ttacatatct tctgctcggt gaccgctgcc
    27661 tacagccatc tgccgccgat tctcgatcgc ttccgcgcgg aacacccgtc ggtggagatt
    27721 aaactcacga ctggtgatgc ggcagatgcg atggaaaagg tcgtcactgg cgaagcggat
    27781 ctggcgattg ccgggaaacc ggagactctg cccggcgcag tggcgttttc gatgctggag
    27841 aatctggcag tagtgctgat tgcccccgcg ctaccctgcc cggtgcgtaa ccaagtttcg
    27901 gcagagaagc cggactggtc tacagtgccg tttatcatgg ccgatcaggg accggtgcgc
    27961 cggcgcattg aactgtggtt tcgacgcaac aaaatcagca acccgatgat ttacgccacg
    28021 gtcggcgggc atgaagcgat ggtgtcgatg gtagcgctcg gctgtggcgt ggccttgttg
    28081 ccggaagtgg tgctggaaaa cagcccggaa ccggtgcgta accgcgtgat gattttagag
    28141 cgcagtgatg aaaaaacgcc gtttgagctg ggcgtgtgcg tacaaaaaaa gcggcttcat
    28201 gagccgctga ttgaggcgtt ctggaagatt ttgccgaacc actaacccgc caaaaagaac
    28261 ctgaacgccg ggttattggt ttcgtcgtgg caatcgtagc ccagctcatt cagccgggtt
    28321 tcgaaatccg gttcatggtc gccaagttcg aacgccgcca gtacgcgccc gtagtcagta
    28381 ccgtggctgc gataatggaa cagcgagatg ttccagtgcg tacccagcgt gttgaggaag
    28441 cgcagcagcg cgcccggtga ttccgggaat tcgaagctgt agaggcgttc ctgcaacgga
    28501 tgcgatggac gcccgccgac catatagcgc acgtgcagct tcgccatttc gtcgtcggag
    28561 agatcaacca cgctgtagcc gccgtcgttg agcatctgca aaatttcttt gcgctcttcg
    28621 aggccgcggc tcaggcgcac gccaacaaaa atgcaggcgt ttttggcatc ggcaaaacgg
    28681 tagttgaact cggtgaccga gcgcccgcca agcagttggc agaatttgag gaagctgccc
    28741 ttctcttccg ggatggtcac cgccaacaac gcttcacgct gttcgcccag ttcgcaacgt
    28801 tccgagacgt agcgcaggcc gtggaagttc acgttggcac cggaaagaat atgcgccagg
    28861 cgttcgccgc gaatattatg ctgggcgata tattttttca tccctgccag cgccagcgcg
    28921 ccagagggtt ccgccaccgc gcgcacatct tcgaacaggt ctttcatcgc cgcacatatg
    28981 gcatcgctat cgacggtgat gatgtcgtcg agatactcct ggcataaacg gaaggtttcg
    29041 tcgccgatgc gtttcaccgc aacaccttcg gcaaacagcc ccacgcgtgg cagatcaacc
    29101 ggatgacctg catccagcgc cgctttcagg caggcggagt cctccgcttc cacggcgatc
    29161 actttgattt gcggcatcag ttgtttgatc agtaccgcca cgcccgccgc cagaccgccg
    29221 ccgccgacgg gtacaaatac gcggtcgaga tgggcgtcct gctggagcag ttccagcgcc
    29281 agcgtgcctt gcccggcgat caccatcgga tggtcgaacg gcggcaccca ggtgaagccc
    29341 tgctgctgtg acagttcgat cgctttcgct tttgcttcat caaagttcgc gccgtgaaga
    29401 agcacttcgc cgccgaagcc gcgcaccgcg tcgactttga tatcggcggt ggcggttggc
    29461 atgacgatca gtgccttcac gcctaaccgt gcggaagaaa acgcgacgcc ctgcgcgtgg
    29521 ttacccgcag aagcggtgat cacgccgtgc gctttctgtt cttccgtcag gcccgccatc
    29581 atggcgtatg cgccgcgcag cttaaagcta tgaactggct gacggtcttc gcgtttcacc
    29641 agaatcacgt tatcgagacg cgacgagagt ttttccattt tttgtagcgg cgtgacctgc
    29701 gccgcttcgt agaccggcgc gcgcagcacc gctcttaaat attcggcacc ttcaggggca
    29761 ccggacaggg gttgtgagtc tgccatcatt aaccccccag tttcgattta tcgcgcaccg
    29821 cacctttgtc ggcgctggtt gccaggctgg cgtaggcacg cagcgcaaag gaaacctgac
    29881 gttcacggtt tttcggcgtc caggctttgt caccccgagc ttcctgcgct tcacgacgcg
    29941 ccgccagttc ggcatcgctt acctgtaact gaatgccacg gttcggaatg tcgatagcga
    30001 taagatcgcc gtcttcaatc aggccaatgc tgccgccgct tgccgcttcc ggtgagacgt
    30061 ggccgataga aagaccggac gtgccgccgg agaagcgtcc gtcggtgatc agcgcacagg
    30121 ctttaccgag acccattgat ttcaggaaac tggttgggta gagcatttcc tgcatgccag
    30181 ggcctccttt cgggccttca tagcggatca ccaccacatc gcccgcgaca actttgccgc
    30241 cgagaatcgc ttctaccgcg tcgtcctggc tttcgtatac tttcgccggg ccggtgaatt
    30301 tgaggatgct gtcatcgacg cccgcggttt taacgatgca gccgttttcc gcaaagttac
    30361 cgtagagcac cgccaggccg ccgtctttgc tgtaggcgtg ttccagcgag cggatacagc
    30421 cattggcgcg atcgtcatcg agagaatccc aacggcaatc ctgcgagaat gcctgtgtag
    30481 tacgaatgcc agcagggcct gcgcggaaca tattttttac cgcatcatcc tgggtcacga
    30541 tgatgtcgta ttgttccagc gtttgcggca acgtcaggcc aagtacattt ttcacatcac
    30601 ggttcagtaa ccccgcacga tccagctcgc cgagaatacc gataacacca ccagcacggt
    30661 gaacatcttc catatggtat ttttgggtgc tcggcgcaac tttacacagc tgcggtactt
    30721 tgcgggagag cttatcgata tcactcatgg tgaagtcgat ttccgcttcc tgcgccgccg
    30781 ccagcaggtg aagtacggtg ttggtagagc cgcccattgc gatatccaac gtcatggcgt
    30841 tttcaaacgc cgctttgcta gcaatattgc gcggcagtgc actttcgtca ttttgctcgt
    30901 agtaacgttt ggtcaattca acaatgcgtt taccggcatt gaggaacagc tgcttacgat
    30961 cggcgtgagt tgccagcagc gagccgttgc ccggctgcga caggcccagc gcttcggtca
    31021 ggcagttcat tgagttagca gtaaacatcc cggagcagga accgcaggtt ggacaggcgg
    31081 aacgttcaac ctgatcgctc tgggagtcag aaactttcgg gtctgcgccc tggatcatcg
    31141 catcaaccag atcgagctta atgatctgat cggacagttt ggttttcccg gcttccatcg
    31201 ggccgccgga aacaaagatc accggaatat tcaggcgcag ggaagccatc agcatccccg
    31261 gggtgatttt gtcgcagtta gagatgcaga ccatggcgtc ggcgcagtgg gcgttgacca
    31321 tatactcaac ggaatcagcg atcagttcgc gagatggcag tgaataaagc atgcccccgt
    31381 ggcccatagc gatcccatca tccaccgcaa tggtgttgaa ctctttggca acgccgccag
    31441 ccgcttcaat ttgttcggca accagtttac cgagatcgcg cagatggacg tgacccggta
    31501 caaattgggt gaacgagttc acaaccgcga taatcggctt accgaaatcg gcgtcggtca
    31561 ttccggtggc gcgccacagc gcacgagcac ccgccatatt acgaccatga gtggtggtgg
    31621 cggaacggta cttaggcata ctttatttac tcccagtgtc tgtctcgtaa atgggacggt
    31681 gcgtgccgtc ccattttttg tatttattga ttaacttgat ctaaccagcc ccatttatct
    31741 tcggtttcgc cagtgaagag gccgaagaag gcttgctgaa tgcgtttggt aaccgggccg
    31801 caacggcctt caccaacctg aataccatct acactgcgca ctggcgtgat ttctgccgcc
    31861 gtaccggaca taaagacttc atccgccagg tacagggatt cgcgcgacag cacctgttca
    31921 cgcacttcaa ttcccagctc tttcgccagt ttgatgatgg catcacgggt aatacccggc
    31981 agcgcggagg aggtgaacgg tggagtgaac agcacaccgt ctttcacttc aaacaggttt
    32041 tcgcctgcgc cttcagagat gtaaccattc acatccaacg cgataccttc ctgataaccg
    32101 tggcggcgcg cttcgctacc caccagcagg gaagagaggt agttaccacc ggcttttgcc
    32161 gccgtcggga tggtgtttgg tgctgcgcgg ttccaggagg aaaccatcgc atcgatcccc
    32221 tgctccagcg cttctgcgcc cagatacgct ccccacggga aagcggcgat aatcacgtcg
    32281 gttgagtatc ccgctggcgg gtttacgccc atgccaacat caccaacgaa gatcagcgga
    32341 cggatatagg cgctggtgag attgtttttg cggatcacgt cacgacaagc ttccatcagc
    32401 tcatcaatgc tctgcgaaac cgggaagcga tagattttgg cggagtcatg cagacgctgc
    32461 atatgctcac gatggcggaa tacaaccggt cctttgtgcg agtcgtagca acggatgcct
    32521 tcaaaaaccg aggtgccata gtgcagcgcg tgcgacatca catgcacctt cgcgtcttcc
    32581 cagcgaacca tctccccatt gaaccaaatg taatcagctt tcttcgtggt catttttata
    32641 ttccttttgc gctcaggcgc ggatttgttg tgatgtggtt gtgctctggc agatggcaac
    32701 gtgtgcgacg tccaccagtt tatttaactg actaaacagt aagtcgaccg accgtgggct
    32761 ggcaacggtc aattcgatat ttatattttg tgcatcgctg gcggcggcca tattcattga
    32821 gcagacgtgg aaaccacgat gacgcaccac gcgtaaaaca cgttctaagg tttccggatt
    32881 gaagcgagcc gatacattga cctgatgttg catcatgata atttctccaa catttctgaa
    32941 ttactggcgc caggcggcac cagcggccag acgttctcaa gttcgtcgat tgagacatga
    33001 agcaggtatg gcccatcact gttcagcatg gtgtcgagtg ccgcttcaac ctggtcttta
    33061 cgggtgatgt gttggccaag gatgccgaag gcgctggcta acatgaggaa atcggggtta
    33121 tcggtaaggg ttgtttcgct gtatcgttcc tggaaaaaca gttgctgcca ttgtcgaacc
    33181 atccctaacc gttggttatc gagtaagacg attttcaacg gtaactgctt gcgttttacg
    33241 gtgcccagct cttgcacatt catcatgaaa gagccgtcac cggagataca gacgacagta
    33301 tcgttcggtc gcgcgacttg tgcgccaacc gccgccggta aaccgaaacc catggtgcct
    33361 aagccgctgg aggtgatgaa gttttccggg cgagtgtgga cgatgtgctg ggcagcccac
    33421 atctggtgct gccccacatc tgtggtcacg atgcaatccg caggtttacg atccgacagt
    33481 tgttttaaca acaacggcgc gtagatagcg tcaccaggat ggtcgtaacg ccaggcatgt
    33541 tcatcacgca gttgcgcgca gtgttgctgc cagtcatcga tatttaacgg ctgctgtaat
    33601 gctggtaaca gagcatttaa atcaccctgt aatgccacat gtgcctgacg cagcttgttc
    33661 atttctgccg gatcgatatc catatggata acgctggcgt gtggtgcgaa ggtgttcagt
    33721 ttgccggtca cccggtcatc aaaacgtgcg cccacggcga tcagtagatc gcactcctgc
    33781 actgcgaagt tcgccgcttt ggtgccgtgc atccccagca tgcccagata gtacggataa
    33841 tctgcttcta ctgcgcccag ccctttcaac gtacaggtgg caggcatttt tgtggcagcg
    33901 agaaattcac gcaaagccga aactgcctgc gccatgccca cgccaccgcc aacgtacagc
    33961 atcggttttt gcgcttttgc cagcatctga cgcgcttgct cgacttcggc atgtgggaaa
    34021 gtcacttcgt tttcaacggt ggtgaaccac ggttccagat cgccgctggc taactggata
    34081 tcttttggaa tatcgaccaa aaccggacca ggacgaccag agctggcgac gtcaaacgct
    34141 tcggccatga tacgcggtaa ctcttccagt gattgcacca ggaaactgtg cttggtgcag
    34201 gctaacgaca accccagaat atccacctcc tgaaatgcgt cagtgccgat aaacggtgcg
    34261 gatacctgac cggtgatagc aacaacgggg atggaatcta acagcgcgtc cgcaagcccg
    34321 gttatcaggt tggttgcacc tgggccagac gtggcgatgc aaacgccagt tttgccggta
    34381 gcacgggcat aaccgatagc cgccattgct gcaccttgct cgtgtcggca cagcaagtgt
    34441 tccacgccac cgtcatacaa tgcatcgtaa accggcataa ttgcgccacc cggataaccg
    34501 aaaacggtgt ttacaccctg tgcccgcaac gcatgtacca cccactgtgc gccattcata
    34561 gttagttccc cgtcctgaat cttgagaaac agaattttgt gctgttattc attgtctgct
    34621 cctcagttat gtttttaagg tcaaaaaaaa cccccggacc tttcggtgcg ggggtcttag
    34681 ttcgttaagg cttgatctct aagcctttcc tcgtccaagt gcagccccgc acggtgggat
    34741 aataatcacc accacgctaa tcacgaccag gctaatcact cgtagaaggg ctgtcatttt
    34801 gtcttttctt gcatcttgtt cgaaggaatg cctaaagagt taccatagat tttgcgaata
    34861 gcacaagata ttttttggcc gatttttatg atgtctttaa taatttcaag agaaattgtt
    34921 gttttttaaa aagaaaatag tgagtgaaaa aaattcaatt tcagtatgaa tgttgttccc
    34981 ttcctcgtag ttcatccttt gcacgctgca tttttgcgag catcattcca ccgccataac
    35041 aactcaactt cacttgcaca aataaacagg aatcagccac taaaattcac gaattagcgg
    35101 gctacagcag cgatatctta tgcacataat ggtgactcaa ggagggctta tgtcactgtc
    35161 aattgttcat acccgcgcag ccctgggagt aaatgcgccc ccaatcactg ttgaggtaca
    35221 tatcagtaaa ggtctaccag gcttaacgat ggtgggctta ccagaaacaa cggtaaaaga
    35281 agctcgcgat cgcgtgcgca gcgccattat caatagcgga tatgaatatc cggcgaaaaa
    35341 aatcaccatc aaccttgctc cagccgatct accaaaagaa gggggacgat atgatttgcc
    35401 tatcgctatt gcgttactgg cggcctcaga acagcttaca gccaataagt tagatgaata
    35461 tgaattagtc ggagaactgg cgcttacagg cgctctgcgt ggcgttcccg gcgcaatctc
    35521 cagtgcaact gaagctatta agtcgggcag aaaaattatc gtcgcgaaag ataacgaaga
    35581 tgaagtgggg ctaattaacg gtgaaggatg cctgatagcc gatcatctgc aggctgtctg
    35641 cgcgtttctg gaaggtaagc acgctctcga acacccgaaa ccaactaatg cagtatcccg
    35701 ggcgctacaa catgatctca gtgatgttgt cggtcaggag caaggaaagc gaggactgga
    35761 aattaccgcc gctggcgggc acaacctttt actgattgga ccgccgggaa caggtaaaac
    35821 aatgctcgcc agccgtatta atggcctttt gccagattta agcaatgaag aggcactgga
    35881 gagcgctgcg atattaagtc tggtaaatgc tgaatcagta caaaaacaat ggcggcagcg
    35941 cccgttccgc tcacctcatc acagtgcgtc gttaactgcg atggtgggcg gtggtgcaat
    36001 tccagggcca ggtgaaattt cgctggcgca taacggcgtg ctttttcttg atgagctacc
    36061 tgaatttgaa cggcgtacac tggatgcctt gcgagagccg attgaatccg ggcagatcca
    36121 tctttcacgc acacgagcaa aaataaccta tccagcccgt ttccagcttg tcgcggcaat
    36181 gaatcccagc cctaccggac attatcaggg aaaccataac cgctgcacac cagaacagac
    36241 attacgttat ctcaaccggc tctcggggcc ctttctcgac cgcttcgatc tctcactgga
    36301 gatcccatta ccgccccccg gcattttgag taaaacggta gtgccgggag aaaacagcac
    36361 caccgttaaa caacgtgtaa tggccgccag agagcgccaa tttaagcggc agaataaact
    36421 gaacgcctgg ctggataatc cggaaatacg ccaattctgc aagcttgaga gcgaagatgc
    36481 gcagtggctg gaagaaacgc tgatccatct ggggttatcg attcgtgcct ggcagcggtt
    36541 attgaaagtt gcacgaacca ttgctgatat tgatcagtct gacattatca cacgtcagca
    36601 tttgcaggag gcagttagct atcgtgcgat tgaccgtttg ctcatccatc tgcaaaaact
    36661 actgacataa aaaaagggca tttcgccctt tttattaatc gtcagaatcg gtgtagtctt
    36721 cagcaccttc aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttga
    36781 tacgcgtcat atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac
    36841 ggcatactgc tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca
    36901 actcattgaa ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaagtcac
    36961 catgacgaga gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa
    37021 agctttccgc catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta
    37081 ttagttacgc ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg
    37141 cggagagcac tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc
    37201 actttggtcg agcggctgaa tcgctctatc tgacccaatc agccgtgagc tttcgaatca
    37261 gacaactgga aaatcaactg ggtgtgaatc ttttcacccg ccacagaaac aatatccgtt
    37321 taaccgctgc cggagaaaag cttctgcctt atgcagaaac gctcatgagc acctggcagg
    37381 ccgcccgcaa ggaggtggcg catacctcac gacataatga gttttctatc ggtgccagcg
    37441 cctcgctgtg ggagtgtatg cttaatcagt ggctgggacg cttgtatcaa aatcaggatg
    37501 tccatacagg cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagt
    37561 tgcatgaacg ccagcttgat cttcttatta caactgaagc ccccaaaatg gacgaatttt
    37621 gtagtcagtt gctggggtat ttcactttag cgctttatac cagtgcccct tctaaactaa
    37681 agggagatct taactatctg cgactagagt gggggccaga ttttcaacag catgaggcag
    37741 gtttgatcgg tgctgacgaa gttcccattc tgacaaccag ttctgctgaa ctggcacagc
    37801 aacagattgc gacgcttaat ggttgcacct ggctacccgt cagttgggcg cgtaaaaaag
    37861 gcggcctgca taccgttgtc gatagcacaa cactttcacg gccgctttat gccatatggc
    37921 tgcaaaatag cgataaaaat gcgttgattc gcgatctatt gaaaattaac gtgctggatg
    37981 aagtgtatta atctgaatgg ctggcaagga tgccggtaga aggatttact tcggagaggt
    38041 tatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggca ggggcggaga
    38101 gactcgaact cccaacaccc ggttttggag accggtgctc taccaattga actacgcccc
    38161 taattag
//
LOCUS       DADSZD010000025        37723 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.025, whole genome shotgun sequence.
ACCESSION   DADSZD010000025 DADSZD010000000
VERSION     DADSZD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.025"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            181..807
                     /locus_tag="J1Z28_001159"
     CDS             181..807
                     /locus_tag="J1Z28_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBA7716009.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            828..2930
                     /locus_tag="J1Z28_001160"
     CDS             828..2930
                     /locus_tag="J1Z28_001160"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBA7716010.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2943..3581
                     /locus_tag="J1Z28_001161"
     CDS             2943..3581
                     /locus_tag="J1Z28_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBA7716011.1"
                     /translation="MNHQDELPLAEVSEIDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLENIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYHFQEAQIEA
                     AIATMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3645..4313
                     /locus_tag="J1Z28_001162"
     CDS             3645..4313
                     /locus_tag="J1Z28_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7716012.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4310..5095
                     /locus_tag="J1Z28_001163"
     CDS             4310..5095
                     /locus_tag="J1Z28_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBA7716013.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5099..5911
                     /locus_tag="J1Z28_001164"
     CDS             5099..5911
                     /locus_tag="J1Z28_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716014.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATHPGERPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(5923..7527)
                     /locus_tag="J1Z28_001165"
     CDS             complement(5923..7527)
                     /locus_tag="J1Z28_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBA7716015.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNLDLPSETFDLVVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVTFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIITDQALNAEIQKG
                     EVGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEENL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARKRLSFD"
     gene            complement(7653..7958)
                     /locus_tag="J1Z28_001166"
     CDS             complement(7653..7958)
                     /locus_tag="J1Z28_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBA7716016.1"
                     /translation="MATLLQLHFSFNGPFGDTMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNVPLSQINQAKLA"
     gene            complement(8067..8143)
                     /locus_tag="J1Z28_001167"
     tRNA            complement(8067..8143)
                     /locus_tag="J1Z28_001167"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8107..8109),aa:Val,seq:gac)
     gene            complement(8148..8224)
                     /locus_tag="J1Z28_001168"
     tRNA            complement(8148..8224)
                     /locus_tag="J1Z28_001168"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8188..8190),aa:Val,seq:gac)
     gene            8532..9788
                     /locus_tag="J1Z28_001169"
     CDS             8532..9788
                     /locus_tag="J1Z28_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716017.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGARAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="J1Z28_001170"
     CDS             complement(9829..11202)
                     /gene="mdtK"
                     /locus_tag="J1Z28_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBA7716018.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            11417..12058
                     /gene="ribE"
                     /locus_tag="J1Z28_001171"
     CDS             11417..12058
                     /gene="ribE"
                     /locus_tag="J1Z28_001171"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBA7716019.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="J1Z28_001172"
     CDS             complement(12098..13246)
                     /gene="cfa"
                     /locus_tag="J1Z28_001172"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBA7716020.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13537..14748)
                     /locus_tag="J1Z28_001173"
     CDS             complement(13537..14748)
                     /locus_tag="J1Z28_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBA7716021.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14861..15793
                     /locus_tag="J1Z28_001174"
     CDS             14861..15793
                     /locus_tag="J1Z28_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716022.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15790..16815)
                     /gene="purR"
                     /locus_tag="J1Z28_001175"
     CDS             complement(15790..16815)
                     /gene="purR"
                     /locus_tag="J1Z28_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBA7716023.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17114..17203
                     /gene="ynhF"
                     /locus_tag="J1Z28_001176"
     CDS             17114..17203
                     /gene="ynhF"
                     /locus_tag="J1Z28_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBA7716024.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17369..18527
                     /locus_tag="J1Z28_001177"
                     /pseudo
     CDS             17369..18527
                     /locus_tag="J1Z28_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(18673..19254)
                     /gene="sodB"
                     /locus_tag="J1Z28_001178"
     CDS             complement(18673..19254)
                     /gene="sodB"
                     /locus_tag="J1Z28_001178"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBA7716025.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19382..20209)
                     /locus_tag="J1Z28_001179"
     CDS             complement(19382..20209)
                     /locus_tag="J1Z28_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBA7716026.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20544..20891
                     /gene="grxD"
                     /locus_tag="J1Z28_001180"
     CDS             20544..20891
                     /gene="grxD"
                     /locus_tag="J1Z28_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBA7716027.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21263..21910)
                     /gene="rnt"
                     /locus_tag="J1Z28_001181"
     CDS             complement(21263..21910)
                     /gene="rnt"
                     /locus_tag="J1Z28_001181"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBA7716028.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(22013..22420)
                     /gene="gloA"
                     /locus_tag="J1Z28_001182"
     CDS             complement(22013..22420)
                     /gene="gloA"
                     /locus_tag="J1Z28_001182"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBA7716029.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(22501..23598)
                     /gene="nemA"
                     /locus_tag="J1Z28_001183"
     CDS             complement(22501..23598)
                     /gene="nemA"
                     /locus_tag="J1Z28_001183"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBA7716030.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYSGGAEGYTDYPTL"
     gene            complement(23635..24234)
                     /locus_tag="J1Z28_001184"
     CDS             complement(23635..24234)
                     /locus_tag="J1Z28_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7716031.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            24337..24576
                     /locus_tag="J1Z28_001185"
     CDS             24337..24576
                     /locus_tag="J1Z28_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBA7716032.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKPAPSDEPEQPSLF"
     gene            24625..25521
                     /locus_tag="J1Z28_001186"
     CDS             24625..25521
                     /locus_tag="J1Z28_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBA7716033.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            25602..26123
                     /gene="sodC"
                     /locus_tag="J1Z28_001187"
     CDS             25602..26123
                     /gene="sodC"
                     /locus_tag="J1Z28_001187"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBA7716034.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCRPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(26124..28136)
                     /locus_tag="J1Z28_001188"
     CDS             complement(26124..28136)
                     /locus_tag="J1Z28_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA7716035.1"
                     /translation="MNASSWSLRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     ESYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDRARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(28136..28993)
                     /locus_tag="J1Z28_001189"
     CDS             complement(28136..28993)
                     /locus_tag="J1Z28_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7716036.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNFYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHASSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(28996..29232)
                     /locus_tag="J1Z28_001190"
     CDS             complement(28996..29232)
                     /locus_tag="J1Z28_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBA7716037.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            29433..29867
                     /gene="slyA"
                     /locus_tag="J1Z28_001191"
     CDS             29433..29867
                     /gene="slyA"
                     /locus_tag="J1Z28_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBA7716038.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(29914..30381)
                     /gene="slyB"
                     /locus_tag="J1Z28_001192"
     CDS             complement(29914..30381)
                     /gene="slyB"
                     /locus_tag="J1Z28_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBA7716039.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            30655..31764
                     /gene="anmK"
                     /locus_tag="J1Z28_001193"
     CDS             30655..31764
                     /gene="anmK"
                     /locus_tag="J1Z28_001193"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBA7716040.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATVAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            31862..32191
                     /gene="mliC"
                     /locus_tag="J1Z28_001194"
     CDS             31862..32191
                     /gene="mliC"
                     /locus_tag="J1Z28_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBA7716041.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            32250..32906
                     /gene="pdxH"
                     /locus_tag="J1Z28_001195"
     CDS             32250..32906
                     /gene="pdxH"
                     /locus_tag="J1Z28_001195"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBA7716042.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHDQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            33035..34309
                     /gene="tyrS"
                     /locus_tag="J1Z28_001196"
     CDS             33035..34309
                     /gene="tyrS"
                     /locus_tag="J1Z28_001196"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBA7716043.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            34371..35231
                     /gene="pdxY"
                     /locus_tag="J1Z28_001197"
     CDS             34371..35231
                     /gene="pdxY"
                     /locus_tag="J1Z28_001197"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HBA7716044.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(35275..35880)
                     /gene="gstA"
                     /locus_tag="J1Z28_001198"
     CDS             complement(35275..35880)
                     /gene="gstA"
                     /locus_tag="J1Z28_001198"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBA7716045.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(35986..37488)
                     /gene="dtpA"
                     /locus_tag="J1Z28_001199"
     CDS             complement(35986..37488)
                     /gene="dtpA"
                     /locus_tag="J1Z28_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBA7716046.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         9658 a         9087 c         9783 g         9195 t
ORIGIN      
        1 acattatttt ttctcctaac cataaaggat tagtttatca gcgttaaatc ttttctcata
       61 cactatcggg ttagggaaaa tatctggtgt tgaggacagc gtcagcagcg tattagttga
      121 gtgcctaata tcaggctgct gaattgtcag cactgtgttt gcgaagagga tgcgaaataa
      181 atgaacccgg ttgatcgtcc actattaaat attggcttaa cgcgattaga attcttgcgt
      241 atatcaggaa aaggccttgc tggtttaaca attgcccctg ctttactgtc acttttgggt
      301 tgcaaacagg aagatattga tagcggcaca gtagggttga taaatacacc caaaggggtg
      361 ctggtgaccc aacgtgcgcg atgcacgggt tgccaccgct gtgaaatctc ttgtaccaac
      421 ttcaacgatg gctcagtagg gacattcttc tcccgtatca aaatccatcg caattatttc
      481 tttggcgaca acggggttgg ctctggcggt ggcctgtatg gcgatctcaa ctataccgcg
      541 gatacctgcc gtcaatgcaa agagccgcaa tgcatgaacg tctgcccgat tggtgcgatt
      601 acctggcagc agaaagaagg ctgtattacc gtcgatcata aacgttgtat tggctgtagc
      661 gcctgtacta cggcatgccc gtggatgatg gccaccgtaa ataccgaaag taaaaaatcc
      721 tcgaaatgtg tgttatgcgg tgaatgcgca aacgcctgcc caacaggggc gttaaaaatt
      781 atcgagtgga aagatattac tgtgtgaatc ttgcaaagga aaaaatcatg gctaacggtt
      841 ggacaggtaa tatattaaga gtcaatctca cgacaggaaa tattaccctc gaagattcca
      901 gtaagtttaa aagttttgtc ggtggcatgg gcttcggcta caaaattatg tatgacgaag
      961 taccgccagg cacgaaacct ttcgatgaag cgaataaatt agtttttgct accggcccat
     1021 taactggatc tggtgccccc tgtagttctc gcgtaaatat cacttcactt tctactttta
     1081 ccaaaggaaa tttagtcgtc gatgcccata tggggggctt ttttgcagcg caaatgaaat
     1141 tcgctggata cgacgtcatt attatcgaag ggaaagcgaa atcaccggta tggctgaaga
     1201 ttaaagatga caaagttagc cttgaaaaag ccgatttctt atggggaaaa gggacgcgcg
     1261 caacgacgga agaaatttgt cgcttgacca gtccggaaac ctgtgtggcg gctattggtc
     1321 aggctgggga aaaccttgtt cctctctctg gcatgttgaa tagccgtaac cacagcggcg
     1381 gtgcgggaac tggcgcaata atgggttcga aaaacctgaa agcgattgcg gttgaaggga
     1441 cgaaaggggt caacattgcc gatcgtcagg agatgaagcg tctcaacgat tacatgatga
     1501 ctgaacttat cggtgcgaat aacaaccatg tcgtgccaag tacgccgcaa tcgtgggcag
     1561 agtattcaga tcccaagtca cgttggacag cacgtaaagg gctgttttgg ggcgcggctg
     1621 aaggtggtcc gattgaaacg ggggaaattc cgccaggcaa tcagaacacg gtcggctttc
     1681 gtacctataa atccgttttt gacttaggtc cggcggcaga gaaatacaca gtaaaaatga
     1741 gtggctgcca ctcttgcccg atccgttgta tgacccaaat gaatattcct cgggtgaaag
     1801 agtttggcgt gcccagcaca ggtggtaaca cttgtgtagc aaactttgtc cataccacca
     1861 tcttcccgaa cgggccgaaa gattttgaag ataaagacga tggtcgtgtg attggtaacc
     1921 tggttggtct gaatttgttc gatgactacg gcctatggtg taactacggg cagttgcatc
     1981 gcgactttac ttattgttac agcaaaggtg tgttcaagcg tgttctgcct gctgaagagt
     2041 atgcggaaat tcgctgggat caactggaag cgggtgacgt taactttatt aaagattttt
     2101 actaccgtct ggcgcatcgt gtgggtgagt tgagtcacct ggctgatggt tcatatgcca
     2161 tcgcagaacg ctggaatttg ggtgaagagt actggggcta cgcgaaaaat aaactctggt
     2221 cgccgtttgg ctatccggtt caccatgcta atgaagcgtc agcgcaagtc ggttccattg
     2281 ttaactgtat gttcaaccgt gactgcatga cgcataccca tatcaatttt attggttccg
     2341 gcttgccatt gaaactgcag cgtgaagtgg cgaaagagct ttttggttct gaagatgctt
     2401 acgatgaaac caaaaactac acaccgatca atgacgcaaa aattaaatat gctaagtggt
     2461 cgctgttgcg ggtctgtttg cataacgccg tcaccctgtg caactgggtc tggccaatga
     2521 ccgtttcgcc gctgaaaagc cgtaattacc ggggcgatct ggcacttgaa gccaaattct
     2581 tcaaagcgat cactggcgaa gatatgactc aggaaaaatt agatttagct gcagagcgta
     2641 tctttacgtt gcatcgcgcc tacacggtaa aactgatgca aaccaaagat atgcgtaatg
     2701 aacacgatct tatctgttcc tgggtattcg acaaggatcc gcagatcccg gtctttactg
     2761 aaggtacaga caaaatggat cgtgacgata tgcatgcttc gctcacgatg ttctacaaag
     2821 aaatgggctg ggacccacag cttggttgtc cgacccgcga aacattgcag cgtctggggc
     2881 tggaagatat tgccgccgat ctggcagcac acaatctact gcctgcgtaa ggaatgggag
     2941 agatgaacca tcaggatgaa ttgccgttag cggaagtcag cgaaattgat gaggcgaagc
     3001 ggcaatggtt gcaggggatg cgtcaccccg ttgatacggt aaccgaacca gaaccggctg
     3061 aaatcctggc agagttcatt cgccagcatt ctgcggctgg gcagctggtg gcgcgcgctg
     3121 tgtttctctc gccgccctat tcggttgcag aagaggaact gtcagttttg ctggaaaaca
     3181 ttaagcagaa cggagactac gcggatatcg cttgtatgac gggaagccag gatgattact
     3241 attactcaac gcaggccatg agtgaaaact acgcggcgat gtctttgcaa gttgtggagc
     3301 aggacatttg tcgcgccatt gcacacgccg ttcgttttga gtgccagacg tatcctcgtc
     3361 cttacaaagt cgccatgctg atgcaagcgc catatcactt ccaggaagcg cagattgaag
     3421 ccgcgatcgc cacgatggat gttgcacctg aatatgccga tatacggcag gtggagtcat
     3481 cgacggcagt gttgtacttg tttagcgagc gatttatgac ctatggcaag gcgtatggct
     3541 tatgcgaatg gttcgaggta gagcagtttc agaacccctg aaatttcagg gggatgtcat
     3601 ggatagggtg gacagttgca gccactgcat taggggataa tcagatgtcc ttcactcgac
     3661 gcaaatttgt tctggggatg ggaacagtaa tcttttttac tggttctgcg tcttctctgt
     3721 tagcgaatac gaggcaagaa aaggaagttc ggtatgccat gattcatgac gagtcacggt
     3781 gcaatggctg taatatctgc gcccgcgcat gccgtaaaac taaccacgtc cccgctcagg
     3841 gaagccgttt atcgatagca cacattccag tcatcgataa cgacaatgga acgcagtatc
     3901 acttttttcg ccagtcatgt cagcactgcg aagatgctcc ttgcatcgac gtttgcccga
     3961 caggggcgtc gtggcgtgat gagcagggga tcgtgcgggt agaaaaatcg cagtgcatcg
     4021 gctgcagtta ttgcattggc gcttgcccgt accaggttcg ctaccttaac cctgtcacta
     4081 aagtggcaga taaatgtgat ttctgcgctg agacccgact ggcaaaaggc tttccgccca
     4141 tttgcgtcag tgcctgcccg gaacacgcat tgatctttgg tcacgaagat agcccagaaa
     4201 ttcaggcgtg gttgcaggaa aataaatact atcaatatca gctgcctggc gccgggaaac
     4261 cgcatctgta tcgccggttc ggtcaacatt tgattaaaaa ggaaaatgta tgaacccgtc
     4321 gcaacatgct gaacagtttc agagccagtt ggcgaactat gtgccacagt tcacccccca
     4381 attttggccg gtgtggttga tcattgccgg actattgctg gtagggatgt ggttggtgct
     4441 ggggctgcat gccttgcttc gtgctcgtgg cgtgaagaaa tcagccaccg accatggtga
     4501 gaaggtttat ctttacagca aagcggtcag attatggcac tggtcgaatg cgttactctt
     4561 tgtattgttg ctggcaagtg ggctgataaa tcactttgcg ctggtgggcg caactgcggt
     4621 taaaagtctg gttgcggtgc atgaagtttg cggatttttg ttactggcat gctggcttgg
     4681 ctttgttctg atcaatgccg ttgggggtaa tggtcaccac tatcgcattc gtcgtcaggg
     4741 gtggctggag cgagcggcaa aacaaacgcg attctatttg tttggcatta tgcaggggga
     4801 agaacatcct ttcccggcaa caacccagtc taaatttaat cccttacagc aggtcgccta
     4861 tgttggtgtc atgtatggat tgctgccatt gttactattg acggggctgc tgtgtctcta
     4921 tccgcaagcc gtgggagatg tgtttcctgg cgtaagatac tggttattgc aggcgcattt
     4981 tgctctggca tttataagcc tcttttttat cttcggtcat ctttatcttt gcaccacggg
     5041 gcgtacgcca cacgaaacct ttaaaagcat ggtcgatggc tatcaccggc actaacgaat
     5101 gatcattaca cgagcagacc tgcgagaatg gcgtatcgga gcggtgatgt atcgctggtt
     5161 cctgcgccat ttcccacgcg gcggtagtta tgccgatatt catcatgcac tgattgagga
     5221 aggatatacg gactgggcgg agagtctggt tgagtatgcg tggaaaaaat ggctggcgga
     5281 tgaaaacttc gcgcatcagg aagtttcttc tatgcaaaaa ctggccacac atcccggaga
     5341 gagaccattt tgcagtcagt ttgcccgcag cgacgatcat gctcgcatcg gctgttgtga
     5401 ggataacgcc agaatcgcaa ccgctgggta tgctgcacaa attgccagta tgggttatag
     5461 cgtgcggatt ggaagtgtcg gctttaacag tcacattggc agctccggtg aacgcgcacg
     5521 ggttgctgta accggtaatt cttcgcggat aagcagcgct ggagatagca gtcggattgc
     5581 gaataccgga atgcgagtac gcgtttgtac attgggtgaa cggtgtcatg tcgccagtaa
     5641 tggtgacctg gtgcaaatcg ccagctttgg cgcaaacgcc agaattgcca acagcggcga
     5701 caatgtacac atcattgcca gcggtgaaaa ttcaacggta gtgagcacgg gcgtggtcga
     5761 ctcaattatt ctcgggccag gaggaagcgc cgcactggca tatcatgacg gtgaaagagt
     5821 tcgcttcgcc gtcgccattg aaggtgaaaa caatattcga gccggagtcc gctatcgtct
     5881 taatgaacag catcagtttg ttgagtgctg atagctttac ctttaatcaa acgaaagacg
     5941 tttacgtgca cgggaggcag gttttacgac agcccgagcc atcgcctcac caatttctgc
     6001 acatgccgta agcccctgaa cgaaaggctg gtcgtgcatt aaccagggca acgcgccgaa
     6061 cgctacgcgc ccacgaatat cttcgggttg ctgtaacgta taatcttcgc caacatcagg
     6121 gatttcatcc cctgttttct gtaattgttc gcgtagtcca gggaaaggaa tatctttcac
     6181 tttaagcgga cgctgcccgc gggcatcaat aaaaacgtca aacgagtaac tgttgtcttc
     6241 cgttttcagg acggtgcgcg actcgttaat ttccatttcg tagtcttcac cgagagcgag
     6301 aatatgaatg attcccgctt cacgtaaggc caatagtcga cgaatagact ctgaagggat
     6361 tgccgcataa ttatcgatga aaacccgggc aaggccttta ctgaaccgtt tatggtcgtg
     6421 ttcattcaga tgtggaacaa tttcctgtac ggcttcatgc aggcgcagaa tgacataacg
     6481 ccagggaaca gtgtgttttt ctcgtttatt gcgttcgact tcctggagat tttcttctgc
     6541 ccagtcaaat gggtcgcgtt gtttgcgctc ggcaaaccag gcttgagcaa aggaatcgac
     6601 attcaggctc tctaaggcta tgcgttggct ccagtctgga tcagcaaact tgatttcctc
     6661 tactatcaat ctaaataccc gatccaaaag gcccacttcg cctttttgaa tctcagcatt
     6721 taatgcctga tcggtgatga tgtgtaaggg ctcgtaggga atagggcaat agaaatcggc
     6781 ttcgggtaaa atacctgtgc gcgacattaa cgtaatattt agcttttcac ttgcgttatc
     6841 gcggtgaaaa gtgacgtgtt gtttatcatc ttcaatgaac gaaccatgct gaatagccac
     6901 tgccattgcc gcatccagtc cgctcaagga tgttcccata atacccacgt tacacgcatc
     6961 gacctttgct tccatcaggc ctgaccacgg gctgggaaaa tacgttctgg ttgcttcttc
     7021 ttcatcaggc cagacgtgcc ccgttgcgat caccactaaa tcaaacgtct cgctgggtaa
     7081 atccagattt gtagcgagca tgacgccagc atttgtaatt tgcagatcgg taacctggca
     7141 tgattcataa acagccactg caaatttttg ctgtcgtgcc tggtctacta atcgtaaaaa
     7201 ttgatcgcgg aaatattcgc ccagcagaat tcgcggtaaa aactgacgat cgtgcaaggt
     7261 ttctttttta acgccataac gctggagatg gctggcttct tgcttttgta gccattcgag
     7321 atacgtgcaa taaatcggcg gtatttcaat actggcaatg tttgccagca tcagttttga
     7381 gttttcatca tcactgtatg gcatcccgac accggcttcg tcagcctgct cgaagataga
     7441 aatagaaagt ggcgtttgtt gctgtagaag cgagaataag gtgtagatcc ctgtaggccc
     7501 ggcacccaca atagcaattt ttttcattgt tacttacctg tacgcattct ttcgaattta
     7561 gtgttatcag gtaagtttgg ttgagatttt tagaatttga gaaatttcag acagggatga
     7621 aaagggcact aaccgccctt gattaaaatc tgtcaggcaa gttttgcctg attgatttga
     7681 ctcagtggca cattgacatc gaaaactttg gcgacaactt cctcaacgcc gagatttttc
     7741 agtcgggcag tatgtttttc cagataggca agggcgcttt tttcatcagt gaaaaggtag
     7801 atcccaccgg cttcgtggtt cttttcactt tctgtccaaa ccttccacag aaaaccaggt
     7861 tcctgattaa tcgactcagc aagcggttta agctgctcag ccattgtgtc gccaaatggg
     7921 ccattgaaag aaaaatgaag ttgtaacaaa gttgccatag cgattctcct tctgataacg
     7981 catacgaaga cagtgtagat cttttaacgt attggataca tagaagaaac agagggaaaa
     8041 aaggataaga ccagattaag aaaatctggt gcgtccgagt ggactcgaac caccgacccc
     8101 caccatgtca aggtggtgct ctaaccaact gagctacgga cgcaggatgg tgcgttcaat
     8161 tggactcgaa ccaacgaccc ccaccatgtc aaggtggtgc tctaaccaac tgagctatga
     8221 acgcaacgtt gtaggtgaca acggggacga atattagcgg cagagtggga aggtggcaag
     8281 aggcaaaacg taattttctg cgctatttcg accgtttgca gagcttttaa gcaaattggc
     8341 tatattttgt tgatttgcaa gggcgatttt tattcaggat cgcatttaca tctgatacaa
     8401 cccaataatc cgtgcatcag agtttaaaca gagttgtttg ccaccatctc aaatctgtcg
     8461 tggataaatc cttcgtagcc tttacactta ggtagcaata aaggcacaca gatgcagcaa
     8521 aggaataaat catgggatct gatgcgaaaa acttgatgag cgacgggaac gtgcaaattg
     8581 ttaagaccgg cgaggttatt ggcgcgacgc aacttactga aggtgagtta atagttgaag
     8641 ctggcgcaag agccgaaaat accgtggtca cgggggctgg ctggttgaaa gtggcaaccg
     8701 gtgggatcgc caaatgcaca cagtacggta acaatggcac gctatcggtc agcgacggtg
     8761 ccattgccac agatattgtt cagtccgagg gaggcgcaat tagtctctct acgctcgcta
     8821 cggttaatgg ccgccatccc gaaggtgaat tcagcgttga tcagggttat gcctgcggtt
     8881 tgttgctgga aaatggcggt aacctgcgtg tactggaagg acatcgcgcg gaaaaaatta
     8941 ttctcgatca agagggtggc ctgttggtca atgggacaac ctcagcggtc gtggtagatg
     9001 aaggtggtga attgttggtg tatccaggtg gggaagccag caattgtgag attaatcagg
     9061 gcggcgtttt tatgctggcg gggaaagcca atgatacgtt gcttgctggt ggcaccatga
     9121 ataatctcgg tggtgaagac tctgacacta ttgttgagaa tggagccatc tatcgtctgg
     9181 gtacggatgg tcttcagctc tacagttccg gtaagacgca aaacctgtcc gttaatgtgg
     9241 gtggtcgggc tgaagtgcat gccggtacgc tggaaaatgc ggtaatacaa ggtgggacag
     9301 tgatcctgtt gtcacccacc agcgcggacg aaaattttgt cgtagaggaa gatcgcgcac
     9361 cggttgaact gaccggtagt gttgcattac tggacggcgc ttcaatgatt attggctatg
     9421 gcgcagatct gcaacaatca acgattactg tacagcaggg cggtgtattg attctcgacg
     9481 gcagtacggt aaaaggtgac agtgtcactt ttggtattgg taacatcaat ctgaatggcg
     9541 gaaaactgtg gctgatcact gatgcggcaa cgcaggtgca actgaaagtg aaacgcctgc
     9601 gtggagaggg agccatttgc ctgcaaacca gtgcgaaaga aatttcacct gacttcatca
     9661 atgtgaaagg ggaagttacc ggtgatatac acgttgagat aacagatgcc agtcggcaaa
     9721 ctctgtgtaa ctctctgaaa ttacagccag accaagatgg catcggcgca acgctccagc
     9781 ctgcgtaaaa acacaggcgc aacccgtgaa ggttgcgcct tgtctttatt agcgggatgc
     9841 tcgttgcaga atgatggctg acggcagacg ttgcaggaac cgcatacgca gcatcatcat
     9901 aatggctgcc gacgtcaggc caataataaa gcctatccag aagcctgctg gccccatagg
     9961 ttcaacgacc agatcggtca atgccagaat atagccgctt ggcaagccca gcacccagta
    10021 agccgtaaag gtgatataga aaatggaacg cgtatcttta taaccacgca aaattccact
    10081 gccaatcacc tggatagagt cagaaatctg atataccgcc gccagcaaca tcaaatgtgc
    10141 agccagcgtt acaacctcgg gattgtcgtt gtacaacagg gcgatttgct cccgcagtga
    10201 aaccgtgaat atggccgtca gggttgccat acagacaccc accataagcc cggtccgcgc
    10261 agcggtttgc gcatccagcg ttgagccttg acccagacga taacctacgc ggatggttac
    10321 cgctgccgcc agcgacatgg gaagcacgaa catcagtgaa ctaaagttca gggcaatctg
    10381 atgtcctgcg acatcaacaa taccgagcgg agacactaac agagccacga cggcaaacag
    10441 cgttacttca aagaacagtg ccagcgcaat tggcaaaccg agttgaatca gtcgtttcat
    10501 aaccgcagga tcgggcttcg cggtgccttt ttcgttacga atatcgcgca tcgagcgggc
    10561 gcgtttaatg taagaaacca tggcaaggaa catgacccaa tacaccgccg cagtagccac
    10621 gccacaacca actccaccga gctcaggcat accgaaatga ccataaataa agatatagtt
    10681 aaccggaatg ttcaccagca ggccgataaa gcccatcacc atacccggct tggtttttgc
    10741 cagaccttca cactggttac gggcaacctg gaagaaaaga tagcccggcg cgccccatag
    10801 caacgcacgc agataaccca cggctttgtc cgccagagcc ggatcgatgt tttccatgga
    10861 gcggataatg tagcctgcat tccacagcac cagcataatg agaacggaaa caaaacccgc
    10921 cagccagaaa ccttgtcgca cctgatgcgc aatgcgctca cgtcgaccgg aaccatttaa
    10981 ttgcgcgata accggcgtta atgccagcag cagtccgtga ccaaagagga tcgccggaag
    11041 ccagatagaa gtaccgatag cgaccgccgc catgtcggtg gcactatagc cgcccgccat
    11101 cacggtatcg acaaaaccca tcgcagtttg ggcgatttgc gcgagaatca ccgggattgc
    11161 taatgctaat aacagacgcg cttcactgat atacttctgc acgtgaacac cttttatttg
    11221 tagttatatg agagactaaa aagccacctg aatgggcagc aaaaagaaga agcaggggaa
    11281 attcagtcta ttgtagcgag gatttattaa ttctccagtg aaaaaatagc cattcttacc
    11341 ccttcactgg taagggcgtt ttccacctgc tattgtgctg aacagaatgt cttaactgat
    11401 ttcaggagtt gtaagtatgt ttacggggat tgtacagggc accgcaaaac tggtgtcgat
    11461 tgacgagaaa ccaaattttc gcacgcatgt ggtggagtta cccgaccaca tgctggacgg
    11521 tctggaaacc ggtgcttccg tggcgcataa cggttgctgc ctgaccgtga cggaaattaa
    11581 cggcaaccat gtcagttttg acctgatgaa agaaacgtta cgcattacta atcttggcga
    11641 tttaaaagtg ggggattggg taaacgttga gcgtgcggcg aaattcagcg atgaaattgg
    11701 cggacactta atgtcaggtc atattatgac caccgctgaa gtagcgaaaa tattaacctc
    11761 ggaaaataat cgtcagatct ggtttaaagt ccaggatagt cagttgatga aatatatcct
    11821 gtacaaagga tttattggca tcgacggtat tagcctgacc gtcggcgaag tcacgccaac
    11881 gcgtttttgc gtccatttaa ttccggaaac actggaacgc acgactcttg ggaagaaaaa
    11941 acttggcgca cgcgtcaaca ttgaaatcga tccacaaact caggcagtgg ttgatacggt
    12001 agaacgtgtg ctggcggcac gagaaaatgc catgaatcaa ccaggtacag aagcctgatc
    12061 tgaaatcgcc cccggagagg gggcgataga atagccttta gcgagcgact cgaaggccgt
    12121 tttcaacacc gcgtgagaac acgacctgcc agagctgaat atcacgggcg cggaaagcac
    12181 ctgcacaggc attcagataa taggtaaaca tccgtttaaa gcgttcactg tagttatccg
    12241 caatttctgg ccatgcggcg aggaatcgtt cataccacgc catcaatgta gtatcgtaat
    12301 cagcaccgaa gttatgccag tcttccatca caaagtgggg ttcgctggac tgagcaatct
    12361 ggcgtacaga gggcaggcaa ccgttcggaa aaatatattt attaatccag ggatcaacat
    12421 tcagatcggt ttttttcgaa ccgatggtat ggagcaggaa tataccttcc ggtttcaaat
    12481 tacgatccac caccgcaaaa taggtatcgt aatttttcgg tccgacgtgt tcgaacatcc
    12541 ccacagaaac aatacgatca aactggtcgt tcaggtcacg ataatcttgc agcaaaatgg
    12601 tgacatccag gccttcacag cgttcctgag ccattttttg ctgttcggca gaaatggtga
    12661 cgccgaccac gcttacgtca taattagacg ccatgtagtg cgccagtcca ccccagccac
    12721 agccaatatc cagtacgcgc atccctggtt ttaactgcaa tttttcacaa atcattttga
    12781 gcttcgcctg ctgggcagat tccagattat cggcatcttt ccagtaagca caggaatatt
    12841 gcatgaaggg atcaagcatg cggctgaaca agtcattacc caaatcgtaa tgctctttgc
    12901 cgactatcca ggcacgtttt ttactctgca gattgaagag acgagcgccg gcaatacgca
    12961 gcgtgtcttt gaaatgatgg ggaagttggt tctcgagacc tgcgcgtaag actttgctaa
    13021 aaaacatatc cagtcggtca cattcccacc agccatccat ataactttcg cctaacccca
    13081 aagaaccttc ctgcagaacg cgtttaaaaa aatcggggtt tttgacacga atatccgccg
    13141 gggcagaacc gttaatggct ataccggcac ggctaagtaa ttcgttggcg atacggtacc
    13201 agttatcatc cggtacactg acttcttcta tacacgatga actcatagtt tctccatcac
    13261 tggtcggtga tcagaacctt aaaacagcgt agacgctttt tgggctttgt gagaaatctc
    13321 gcagaaaaaa ccgcgagtct ataatccgac gtagaacaga gggaaaggag gaacccttgc
    13381 cgaaatgacc atccatagtg agtcgggaac gatcctgtcc ccgtaataac ataatattta
    13441 tcgtagtttt aacaactcga aattagtata ggccgctaat ttcaatcata caacaaaatt
    13501 ttgttagcag cctaagtata agtatatcga tatagatcag tgtgattcgc tatgagcgac
    13561 ttcggcattg ccatgattct ggcagccaac ttcttcacaa cgttgcatca tgtaacccag
    13621 agcgaccagc actactgttg ataacatcac gctggtggtg gtgagcaatg gcgtgctgat
    13681 actgataagc caggaaacta ccagacttgc gaggaagcac agacccagtt gtagagtgtt
    13741 ctgcaacgct gcggcgcgac cagttgcatg tgggaaggga cgcagcgcct gggcgacaac
    13801 aatcgggtag atcgcgccat tggcaatcgc catcacacag aatgggatca ggatttcgac
    13861 caacgacaca tggctaatga agcccgcagc ccaggtcgca atgacgctga cagcaaacag
    13921 taccagcaac cacggtaata actgcttgcc ttgccatttc tgcagcgcgg cgcgacagcc
    13981 ataaccacca atcagaaacg cgatagtttg cgggacataa cttaaaccaa taactgccgg
    14041 gctgtaaccc atttcactaa ggatgaacgg tgaaccggtc agccatgcaa aaaaactggc
    14101 tgaacaggcc gcgtatatca gcacgttgcc gcgataggtt ttagaacgta gcaggtcggt
    14161 aaaggtcaga ccatcctgac tattgttacg ggccttcgtc gtgggtttga gccagaaaat
    14221 aggcagaatc agcaccacgg taatggcaaa cagggtggcg aaaatcgcct gccaggaaaa
    14281 atggaccagc agccagcttc ctaacagagg agccagtgcc ggagatagac ccaccagcgg
    14341 catgatggtc gcaaaaatac ggttaacttt ctgtgaagga taataatctg tcactaatgc
    14401 ttgccagata accgccgcgg cgcagacacc cacagcctgt acaaaacgca atatcagcag
    14461 cgtggcggcg ttttctaccc acagcatccc cagactacct aacgcaaaaa ttgtcaggcc
    14521 gatgaataac accggtttac gaccataacg gtcggagagt ggcccccaca gaagctgggc
    14581 tgcggcaaaa ccggcaagga acagactaag gctggcactg acagcagacg caggcgtttg
    14641 caggtcggcc tgtatggcgg cgaaagcagg cagatacata tcggttgcca gaaaaccgag
    14701 aacgctcaaa cctgccagcc agactaaaaa tcttttccca ggttgcatgt atattctcta
    14761 aatctctcaa ttaaacaccg ccgcaaagtg tagggagtga aattagtgtt gtgaaacggt
    14821 aatatttgat gattggtttc aaaaattttg caggcagact atgtggtcag aatattctct
    14881 ggaagttgtt gatgcggtag cgcgcaatgg tagttttagc gccgcagccc aggagttgca
    14941 tcgcgttcct tctgcggtca gctacaccgt gcgtcagctg gaagagtggc tggcggtgcc
    15001 gctttttgaa cgacgtcacc gtgatgtgga actgaccgct gccggggcgt ggtttctcaa
    15061 agaaggacgc tctgttgtca aaaaaatgca gatcacccgc cagcaatgtc agcagatagc
    15121 gaacggctgg cgcggtcagt tagctatcgc agtggataat attgtcaggc cagagcgaac
    15181 acggcagatg atcgttgatt tttatcgcca ttttgatgat gtcgaacttc ttgtctttca
    15241 ggaagtgttc aacggtgtct gggatgcact ttccgacggg cgcgtggaac tggcgattgg
    15301 cgcaacacgc gcgattccgg taggcggtcg ttatgccttc cgggatatgg ggatgttaag
    15361 ctggagttgc gttgttgcca gccaccaccc gctggcgttg atggatggcc cgttcagcga
    15421 tgatacgttg cgcaactggc cgtcattggt gcgcgaagac acctcgcgaa cgctacctaa
    15481 acgtattacc tggttgctgg ataaccaaaa aagacttgtc gtgcctgact gggaatcatc
    15541 ggcaacctgt atctcggcag gattatgcat agggatggtg ccaacacatt tcgccaaacc
    15601 gtggcttaat gaggggaagt gggtagcact agaactggaa aaccccttcc cggattcggc
    15661 atgttgtctg acatggcagc aaaatgatat gtcgcctgcg ttaacctggt tgcttgagta
    15721 tctcggcgat agtgagacgc tgaataagga gtggctgcgg gagccggaag agactcccgc
    15781 aacgggtgat taacgacgat agtcgcggaa cgggccgtca gccacggagc ggcgttcaat
    15841 caaccgcgga tgcacttcaa tggactgcgg ttcttcacgt ttgttgacga tacgatccaa
    15901 cagcatgttg aacgctgttt cgcccagcga atcttttggc tgatggatcg tggtcagcgc
    15961 cggcgtaaaa tagcgggcgt tgcgcacgtt atcataaccg atcagcgaaa catcctgcgg
    16021 cacgcgcaga cccatttcat cagcagcaca aagtgcgccc attgccatga tatcgccacc
    16081 acagaagacg gcagtagggc gatgcgactg cgacaggatt tgctgcatgg cgcgataacc
    16141 ggattcaggt tcaaagtcac cctgcacaat ccagctttcc ggcactttga tcatcgcttc
    16201 ttccatcgcc ttcataaaac cggcaaggcg gcctgcgccg gtgttacgtt ccagcgggcc
    16261 agggataacg ccgatttcgc gatgaccgcg ttcaatcaga taacgcccgg ccatgtagcc
    16321 gccttcgaat gcgttatcaa tgaccgcatc ggtgaagtca gcttttgctt caccccagtc
    16381 catcaccacc attgggatat ggcgatactc ttccagcatc gccagcaacg gctctgggta
    16441 ctcagaacac atcaccagca gaccatcgac gcgtttttgc gccatcatcg acagataagc
    16501 ccgctgtttc tcaagattgt tccacgcatt gcccagaatc agggtgtaac ctttctggaa
    16561 gcaatttttt tcgaccgctt caatgatctc ggcaaaatag gccgcttcgc tgctggtcgc
    16621 cagcaaaccg atagacttgg tgtggttaac cttcaggcta cgcgccaccg cgctagggga
    16681 gtagtgtaat tctttgattg ctgcccacac ggcattgcgc gtttcttcag cgacgaaacg
    16741 tgttttgttg atcacgtgtg acacagttgt agtggaaacg tttgctcgtt tcgctacatc
    16801 ttttattgtt gccattccat ttcactccag accctaactt cagctcctgc aaattcgcaa
    16861 ggtaaacgtt tgcctttaca caccttacgc acaacaaaaa tgttgcggta cgccggaaaa
    16921 acgacacgga gcgtcaggaa ggggtcaatg gccagtacgc aaataatgta agcgtggaat
    16981 tttggccgat cttgacgaaa aggggaagta gtaaaaacac ttatcattat aaatgacggg
    17041 agattttttc atcacagtgt gtaaaaatgc gtaatatcac tttttgtgtg gtaataaaaa
    17101 aggagaaaac ttgatgagca ccgaccttaa attttcactg gtaacaacga ttatcgtcct
    17161 cggtttgatc gtggccgtgg gtttgactgc cgcgctgcac tgatttcctc tcgagggagc
    17221 gcgttctccc tcgcatctcc ttcctgtttg ttagcaaaat ggcaaaagtg ttttctcttt
    17281 ttgttatttt catttttttg tgattactgt catgctttcg ccactttaat gaatatcgcg
    17341 taatgacgcg aagaggtgga gataaggcat gaaaattaac tatccgttgc tggcgctggt
    17401 ttggtatcgg gacaacggag ttctcgccaa tgggcttgtt gcccgtcatt gcgcgcggtg
    17461 tggatgtctc gattcccgct gccggaatgt taatcagtgc ttatgcagtt ggcgtaatgg
    17521 ttggcgcgcc gctgatgacg cttctacttt ctcatcgtgc ccgccgcagt gcgttgattt
    17581 tcctgatggc gattttcacg cttggcaacg tactttccgc catcgcgccg gattatatga
    17641 ccctgatgct ttcacgcatt ttgaccagcc tgaatcacgg ggcatttttt ggtttgggtt
    17701 cagtcgtggc cgcaagcgta gtgccaaaac ataaacaggc tagcgcagtt gccactatgt
    17761 ttatggggtt aaccctggca aatatcggtg gcgtgccggc ggcgacctgg ttgggcgaaa
    17821 ccatcggctg gcggatgtca tttctggcaa cagcggggct gggcgtgatt tcaatggtaa
    17881 gcctgttctt ctcattacct aaaggtggcg caggggcgcg acctgaagtg aaaaaagagc
    17941 tggcggtatt aatgcgtccg caggtgctgt ctgcattgct gacgacggta ctgggcgctg
    18001 gtgcaatgtt taccctctac acctatatct ctccggtacc gcaaagtatt acccacgtaa
    18061 cgccggtgtt cgtcacggca atgctggtgc tgattggtgt cggattttct atcggtaact
    18121 atctcggcgg caaactggca gatcgttcag ttaacggcac gttgaaaggc tttttgttgt
    18181 tgctgatggt gattatgctg gtaatcccgt tcctggcccg caatgagttc ggcgcagcta
    18241 tgagcatggt agtgtgggga gctgcaacct ttgcggtcgt accgccgtta cagatgcgcg
    18301 tgatgcgtgt cgccagtgaa gcgccaggtc tgtcttcatc agtcaatatt ggtgccttta
    18361 atcttggaaa tgcgctggga gcagctgctg gtggtgcggt aatttccgct gggctgggat
    18421 acagttttgt gccggtgatg ggggcgattg tcgcgggact ggcattattg ctggtgttta
    18481 tgtcagtcag aaaacaacct gaaacagttt gcgttgccaa cagctaatta aacaaaaacg
    18541 cagaaggata accttctgcg ttacagttgc tggcaacgtg cgatttatct tgccgaatgc
    18601 gctgttaacg cctcatccgt gttggcatgg cgcgcgaagc gcatcaggta atgttgcatt
    18661 tgccatcagt tattatgcag cgagattttt cgctacgaat tcccagttca ccagcgccca
    18721 gaagtgctcc agatagccag gacgtgcatt gcgatagtcg atgtaataag cgtgttccca
    18781 gacatcaacg gtcagcagcg gagtcgcatc ggtggtcagt ggagtacccg cgttagaggt
    18841 tgaaacgata gccagtttgc catcgctgtt tttcaccagc caggtccagc cagaaccaaa
    18901 gtttttgatc gctgcatcag taaactgagc tttgaaatcg gcaaagctgc caaaagatgc
    18961 ggcgatagct tcagcgactt ttccagtcgg ttcgccaccc gcgttcggtg ccaggcagtt
    19021 ccagtagaaa gtatggttcc agacctgagc tgcgttgttg aatacgccac cttcagagct
    19081 gcgaataatc tcttccagtg atttaccttc aaacgcggta cctttgatca ggttgttcag
    19141 gttagtgaca taagtctgat gatgcttgcc gtagtgatac tcgatggttt ccgcagaaat
    19201 gtgcggtgcc agagcatctt tagcatatgg tagtgcaggt aattcgaatg acattgctac
    19261 tctcctttat tattaatttg catacgtaca atagccttat tgtgcgtatg gatagggtag
    19321 caaattaaaa ggtgagacaa aagatgaact taccctgttg ccgtaacaac agggtaaagt
    19381 tttagcgaag tgtttttggg gtcattaccc ggcgagcgcc aacatagtgg cgctgccagt
    19441 agtcttcact gagagaagtg atttgaattt cttggcccgt gcgcggtgac tgaataaatt
    19501 tgccgttgcc gacatacacg ccgacatgat cggctgtacc gcgtccctga gtacggaaga
    19561 agaccaggtc gccgtttttc agttcactac gttcgattgg cgctgcatcg cgcaggtgat
    19621 acatttcatt cgccgtacgc ggaatacgaa ttttcaccaa atctttataa gcgtaataaa
    19681 ccagaccgct gcaatcaaaa ccggtacgcg gtgaactgcc accccaacga tatggcttac
    19741 caatttgctg catcagttta ttcattgcca cttttgtggc tttttgcacc ttcgctttgt
    19801 gcgcgtcggc aagggttcct gacgcggcat ttttgacttt cacacaatgc gatttatagc
    19861 ccttacgttt ggtacacttt tcggtaaagc tggctgaggc cgttttattg acggcgtgtt
    19921 tgctgcgagt tttggcgttt ttagaagcgg taatggaaga ttttttaacg gaagaagcgg
    19981 ttttgctttt ggtcgtactt ttactggcgg cttttttcgc tgttttctgg ctggctttct
    20041 gggtcttttg ggtggttgcc gtgcttttct ttttatctgc ttttttggtg atatgagtgg
    20101 tagcagaact ctccctggct tgctttgaag catgggccat aggcgttaaa ggcagggtgg
    20161 taaaaagtaa agcacagagc gtgatcgaaa tacggtttat ccgcgccact gagcgtccct
    20221 ctgttaaggc aggtctatca tgcctgcgtc tgatttacaa aacttgatga ttctaatgca
    20281 tagaagaaga gaaagttaat agcttttatt gttaaaaaat acgtttcgtt ggcgagcgca
    20341 aaaaaatgca gcagtgaaag ctaaaaatgg ttataaatac gccagtttgc cgtgcatcag
    20401 ggaagaaacg aatatttgaa atgcaacaaa tagagtaaag ggataaaata gctaaagcgt
    20461 gatgaaaatg ttattttccg atgccgcact gacccgctac aatgtcagat tactgccgta
    20521 aagaagtttg aggaagcaag acaatgagca ccactatcga aaaaatccaa cgccagattg
    20581 ctgaaaaccc gatcctgctg tacatgaaag gttcaccgaa actgccgagc tgcggtttct
    20641 ctgcccaggc agtccaggcg cttgccgcat gtggcgaacg ttttgcctat gttgatattc
    20701 tgcagaatcc ggacattcgt gcggaactgc cgaaatatgc gaactggccg accttcccgc
    20761 aactgtgggt tgacggcgag ctggtgggcg gttgtgatat cgtgatcgaa atgtatcagc
    20821 gtggtgaact gcagcagctg atcaaagaaa ctgccgctaa atacaagtct gaagagccgg
    20881 acgcggaata atccgtcctg caatactgac ggatgcgaac gggcatccgt cagtcctttc
    20941 tctatcccca atccagccct tgtggcgaac ttgaaaatcc cgcctcacgc agcagcgtaa
    21001 acatcggcgt ttgccggacc ggtaggtcat tcacttctgt tagcgtaaag cgcagccgtg
    21061 gttcgcgact ggacagctgt cggctgttta aaggcgcgaa gaaaccagtc gcgggtcgcc
    21121 ggtgaaaaca cgtccggcag gggcgatgaa gggtttagat tatctgccat taacgctatt
    21181 ccccgtgaat atcatgcatg atgcgtggaa tagcctcact atagatgcct gaatcattgc
    21241 atgtcatcag gcatcgactc gattacacct cttcggcggc aggcagcggc cagcctccca
    21301 gacgtttcca gcggttgacg atttcacaaa acagcacagc agtgcgttcg gtgtcgtaca
    21361 acgcggagtg cgcctgggtg ctgtcgaagt ccatgccagc ggtctggcaa gcctttgaca
    21421 atacggtttg tccgagtgcc agcccggcca gtgcagcagt gtcaaaagtg gcgaaagggt
    21481 ggaacgggtt acgtttcagt gaggcgcgtt ctgcggcggc catcataaag ctgtgatcga
    21541 aattggcgtt gtgcgccacc ataatggcgc ggttacagcc gctcgcttta atacctttac
    21601 gtacaacttt aaaaatttcg tgcagcgcct cgtactcgct gaccgcgccg cgatcgggat
    21661 cgttcggatc aatgccattg aaggcaaggg cctctggttg caaatttgcg ccgacaaatg
    21721 gttcgacgtg gaaatgtaat gtggtgtccg gcatcagcca gccttgttca tccattttca
    21781 gggtgatggc ggcaatctca agcagcgcat cggttttggc gttaaatccg gctgtttcaa
    21841 catcgatcac aacaggataa aaaccacgaa aacggtcgca cagaccggta agttgagcgt
    21901 tatcggacat ctgattctct taatacggat aaattgcagc gcgcattatg acaaatatta
    21961 cttgtcgatg cagtaaagat gcgggcgcga tgagttcacg cccggcagga gattagttgc
    22021 ccagaccgcg accggcgtct ttctcttcga ttaattcaat tttgtaaccg tccggatctt
    22081 ccacaaatgc gataaccgta gtaccgcctt ttaccggacc cgcttcacgg gttacgttac
    22141 ccccgttttg acggattttt tcgcacgctt cagcggcgtt atctacgcta agcgcgatgt
    22201 gaccataagc agtgccgagt tcgtatttat ccacgcccca gttgtaggtc agttcaatca
    22261 ctgcttcttc ggtttccggg ccgtaaccaa caaacgccag tgagtatttg tattccgggt
    22321 tttcgctggt acgcagcagt ttcatgccca gcacgttggt ataaaaatcg atggagcgtt
    22381 gcagatcgcc aacgcgcagc atggtatgaa gaagacgcat ctttatatcc tcaaaatggc
    22441 taacagattc aaaaagttgt tttagtatag cggcggcttt gcgccgctcg caatgttgga
    22501 ttacagcgtc gggtaatcag tgtaaccttc agcaccgcca ctataaaaac tttcggcacg
    22561 ctgtgggtta agctcagctt tgcgctgcaa gcgggcgacc agatccgggt tcgcaatcca
    22621 gtcacgacca aatgctaccg catcaattaa ccctttgccg atcagcgttt cagctttttc
    22681 tactgtgtat gcacctgcgc cgataatcgg accgtggaaa cgggcgcgta ctttttcgcg
    22741 aaacgcatca gtatatggtt cacctcccgc ccaatctggt tctgacatat gaagataagc
    22801 aatgccgcgt ttacccagtt gttcaatcag atacagtgca tcggcttctt cattcgggcc
    22861 gttatccgtg ttctggaaag taccgattgg cgaaatgcga atgccaatgc gatcggcacc
    22921 ccattcttca atcccggcat cgaccacttc cagcaccaga cgcgcgcgat tttccacgct
    22981 gccaccgtac tgatcggtac gatggtttga agaaggggaa aggaactgat gcagcaaata
    23041 accgtgagca gagtggagct ctaccagatc aaaaccggct tcacgcgcgt tagcaatggc
    23101 ctgacggaaa tcattgacga tacccggaat ctctcccagt tcaagcgcac gcggcatgga
    23161 tgtttcaaca cggatcgcct gaccattttc atcgcgcaga gaagtacgtg ttcccgcgct
    23221 aagtgctgaa ggcgctaccg gtgcctgacc gccaggttgc aggctggcgt gagaaatgcg
    23281 tccggtgtgc cacagctgta cggccatatg accattttca gcatgaacgc cagcggtaat
    23341 ttttttccat gcggcaattt gttcctggct atggatgcca ggtgcacctg cgtatccttt
    23401 tgcctgggca gaaatttgcg tggcttcgct aataatcaaa ccggcactgg cacgttggcg
    23461 atagtattcc gccatcaacg gggtagggat gtcacccggt tcaatactgc gcagacgcgt
    23521 cagcggtgcc ataaaaatac gatttgccgc cgtgatcgcg cccactttca gtggggaata
    23581 cagtttttca gatgacataa aggctcccgg tcgtctggtg ataaaaaatg cctgctaaac
    23641 ggcaggcgtc gcaataatgt tttttacatg ggccagcgcg ttttccagtg gctcgaaact
    23701 gcgcgaaatt ttggcctgca gattcgcgcc aagccacagt gcgtaaagca cttgtgcctg
    23761 ttgcagcggt tcgccacaaa aggttaaaca atggttctca cggccatttt ccagcgcctg
    23821 agagagcagg gcgatcacgc cgcgagcccc tttatccatt gcgctgcgca tatcttctga
    23881 cagatcgcac acttcggcag agagttttac tgtcaggcaa ccactgatgg ttccatgttg
    23941 gcaaaactgg ttcagtgttt gctggtaata agccagtatg cggtcgcggt agttaccttc
    24001 gccggattgc agcaactcag tcagtcgctg gtgatatgcg gcgtaatggc gctcaagcat
    24061 agcaacgcca aacgcttctt tagaacgaaa gtagtgatag aaggaccctt tcggcacttc
    24121 agcggttttt aggagttcgc ttagccccat cccggtgaat ccacgttgca ggcaaagttg
    24181 ctcgcccgtc gccaggagat gttcgcgagt atcatgttcg gtgtgtttgt tcatgcgttg
    24241 gagtgtagta gaccagtcgg tctagtgcaa gcaaagttgc tactttggac attaacagct
    24301 tcaataatgg taataatctt atctgaggag ggcgaggtga cggagcagtt agagttcttt
    24361 cccgtccaga gcccgtgccg gggaatttgc cagtctgacg aacggggatt ttgtcgcggc
    24421 tgttttcgta gccgggatga gcgttttaac tggaataaga tgagcgatgg cgaaaaacaa
    24481 gaagtgttgc gcctttgtcg gcagcgatta atgcgcaaat tacgtgtaaa taagccagcg
    24541 ccatcagacg aaccggagca accatcactc ttttgaccct aatcttgcgt atactcctct
    24601 catatctgat aagaggaagc gattatggtt cagcgtatta ctattgcgcc gcaaggcccg
    24661 gagttttccc gttttgtgat gggctactgg cgattgatgg actggaacat gtccgcccgc
    24721 cagttggtca gttttattga agagcatctg gatctcggcg tgaccaccgt ggaccatgct
    24781 gatatttatg gtggctatca gtgcgaagcg gcgtttggcg aggcgctgaa actggcacct
    24841 cacctgcgtg aacggatgga aatcgtcagt aaatgcggta tcgcgacgac cgcgtgtgaa
    24901 gaaaacgtca ttggccatta catcactgac cgcgatcaca tcattaagag cgccgaacag
    24961 tcgctaatta atctcgcgac cgatcatctg gatctgctgt taatccaccg accagacccg
    25021 ttaatggatg ccgatgaagt ggcggacgcc tttaaacatc tgcatcagag cggcaaagtg
    25081 cgtcattttg gtgtatcaaa ctttacacct gcgcaatttg cgctgttgca atcacgcctg
    25141 ccatttactc ttgccactaa tcaggtggaa atatccccgg tgcatcagcc gttactgctg
    25201 gatggcacgc tcgaccaact gcaacaactg cgtattcgtc cgatggcgtg gtcctgcctt
    25261 ggtggtggtc gtctgtttaa tgatgattat ttccagccgc tgcgtgatga actggctgtg
    25321 gtggcagagg agttaaacgc gggctcgatt gaacaggtag tttacgcctg ggtactacgt
    25381 ttaccgtcgc agccgctgcc aattatcggc tcaggtaaaa ttgagcgcgt acgggcagct
    25441 gtcgaagcag aaacactgaa aatgacccgt caacaatggt ttcgtatccg taaagcggca
    25501 ctaggttacg acgtaccgta agcactttta ggaatagccg ccgttcaaaa atgtgtcact
    25561 ggtttacact tattcaggaa tgcacaatga acggaggtcc tatgaaacgt tttagtctgg
    25621 ccattctggc gctggttgtt gcgaccggcg cacaagctgc cagtgaaaaa gtcgagatga
    25681 acctcgtcac gtcgcaaggg gttgggcagt caattggtag cgtcaccatt actgaaaccg
    25741 ataaaggtct ggagttttcg cccgatctga aagcattacc tcccggtgaa catggcttcc
    25801 atattcatgc caaaggaagc tgccggccag ccaccaaaga tggcaaagcc agcgccgcag
    25861 aatccgcagg cgggcatctt gatccacaaa ataccggtaa acatgaaggg ccagaaggtg
    25921 ccgggcattt aggcgatctg cctgcactgg tagtcaataa tgacggcaag gcgaccgatg
    25981 ccgtcatcgc gcctcgtctg aaatcactgg atgaagtcaa agacaaagcg ctgatggtcc
    26041 acgttggcgg cgataatatg tccgatcaac ccaaaccgct gggcggtggc ggtgaacgct
    26101 atgcctgtgg tgtaattaag taattaagaa gccagcgtac cttgcggcgg tgcttgctca
    26161 agttgcgaaa gcgagcagta cagccgccag acaattgccg ccagttctcg ggcggcaggt
    26221 tgatgatgac gggcaaggct gtcgcaaatt cgctgtaatt cttcaagtgt ggccgccagt
    26281 gatttttgct gaacgccacg ctcactcatc actccccgca acagtgaaat acagttatcc
    26341 cgtactcgcg ataacggatc ggaacgcgat tcccagtcgc gcaattgcca gacaacatga
    26401 gaacagttca gcaacactac accccagcgt aataaccaac ggcgagccag cgcatcctgg
    26461 ctgttactca actgactgac gtgatgataa gtgagcgatt caaattcgct ttcactcagt
    26521 gttggatggc ggcttagttg atcgacaaaa tcccggcgca gcgcgcgaat atggcggcgg
    26581 cttttacgag catccgatcc tggacgcaga atggcgaacg ctaaccacgc caacgcgacg
    26641 ccaacaattt ttgccagatt atcgttaaga aaatcagcaa aatcatacac cgggggatta
    26701 gtgacagcga taaacgaacc cataaaaaca atcagttgcc cccacagtgc ggcaaatttg
    26761 ggcatctgca atttaagaag ctgcattgtc gccagcagtg gaaagagaaa cagtaaaaat
    26821 tgccacagat cgctaatctg caccatcagg ccaaatttga ccacaaagct gaatagcgaa
    26881 agtaacacca gcgtgcgcat cagcagtgac aacgacttaa acggtgccgc gacggcggag
    26941 tagagtacgc agctaattgc tgccagcgtt aaagcattgg caccggcatc ccattgcgaa
    27001 gcaatactcc atgcgccaat catcatcaac gtacaaaatg tacgcagccc gctccacata
    27061 gcttcggcgt tatcggtatg acgagccagg ccggatgtcc gtgggagtct ggcacgatca
    27121 tctacaccgc tttgcagacg atgtaattcc tgactacttt gcagataaag gcggcaaaaa
    27181 taacgtagtc gctgccagaa ggcgacgtgc cggtagtcgg cgacgttggt cgggcgtagc
    27241 ggggagataa tacgtgcgac ggtgtaaaca tctgtttgcg aactggcgag cgccgtcagc
    27301 agctgttcga gaatttctcg cgtggcatct ggcggtgagg gccagttgag caacatacgt
    27361 cgcaggctgg agatgacact ggtcatacgt aattgctggt ggagtagcgc attaagacgc
    27421 gcgttttgct ggcgaaaacg atagtggctc cagaaagcct ggatacgcag caaattcatg
    27481 gtcagtatct gcccaatcac cccttcatgt gctgcacgaa tggcatcggt tgtttcaggc
    27541 cgccagagta aactggcatg ttccagtaat cgggcgtgca tgtttttcaa tgcggttaaa
    27601 agggctgtag catcggaact gctcggcagg atcatcatca tcatgccgcc gcacaaaata
    27661 ccgactatca cctcgcaaac acgcgcctga gcgatatccc acagctggct ggcctcagta
    27721 atattaacca tcggaaaggc gatgatggca gccgtgtagc ccgccagttg aaatgcatac
    27781 gcgacgttat tcgtgaagtg cgcacaggcc caggtacaaa agccaagcca cgccgacatg
    27841 ctcaatagaa aaaaccacgg ctcattgagc gtatgtccgg caagaagcag tgctgcaatg
    27901 gctccgagca aactgccagc gatgcgtccg aggcttttgc tgatgacacc gccaacggtg
    27961 ggaaagctaa ccaccgcagc cgaggtcatc gcccaatagg actcatcaag attcaaataa
    28021 taggcaaccg tcagcgccag acacatggca atggtattgc gtaacgcata acgccattgc
    28081 gccagcgtgg ccctgaacca gggcaaattg cgcaaggacc atgacgatgc gttcattatt
    28141 gacctacggc aatgctacag gtggtgcccg acaccagcgt gacatcgcct gggactttat
    28201 ccagcgtaaa gcggacggga acgcgttggg cgaggcgaac ccaggggacg ttaggtttca
    28261 catccggaat aaggcttgag tcactttcaa cgctctgatc ataaatcgcc cgaccgatac
    28321 tggacgcgtg accctgtaac gttttgttat cactatagag ggtaatttgt gctggtgcac
    28381 cttcgcggat atggcgcaat ttggtttctt caaaataacc aatgacgtaa aacgagtggc
    28441 tgtcaacaag ggcaaacagc ggtttcccgg tgtcagcgta gtcgccgatg cgggtagtga
    28501 gattagtcac ccatccactc accggggcgc gaatttccgt ttgcgccagt tgccattgtg
    28561 cctgttttag cgtcgcctgt gcggcatcaa cgctggcctg catcgcttta acattgaggt
    28621 tagcggtatc gagttcttcg gcagaaataa aattttgtga gagatgacga cgacgattcg
    28681 cttcgttatt ggcttttgcg aggtcagatt gcgctttcgc cagctgagcc tgtgcgttca
    28741 gctcggcgat ctgaaagggc gttttatcga tcgtgagcag aagatccccc gcgttaacca
    28801 gctggttgtc ttttatattc agctcaacaa tacgtcccga cacctgtggg gtgatagaaa
    28861 cctgttctgc gcgtattttc ccgtcgcgtg tccacggaga ttgcatgtaa aaattccaca
    28921 gccaccatcc ggcaagaacc gcaactaccg ctacaatgat tgttgagaaa tacttaattg
    28981 ttttaattga catagttacc acgcaatcag tattgccaga gcaaggcaaa cgcaaatcgc
    29041 aaacagcgat aaatccatta acaagggatg ccagatgtca ccagcgtaga tccagcctcg
    29101 aagcaggcgg tgtatgacaa gccagatgac gaatccaaat gcgaatgctt tgaaaaacgg
    29161 aggaaagtag acggacgcac cgaacaccag atcttgtaag ggcaatcctg ttgcattgag
    29221 cataaacttc acaaagtaga ttcctttacg acagaatata ttgcgttaga ttaataaata
    29281 ttctttaagt gcgaaaaatt tacgcgcaat ttctgaaaaa tatgtctgac ggtaaccaaa
    29341 tgcagcaata catttgtttt agcaatacaa ttgctgcaca ctattctaaa agccgcataa
    29401 tatcttagca agctaattat aaggagatga aattggaatc gccactaggt tctgatctgg
    29461 cacggttggt gcgcatatgg cgtgctctga tagaccatcg cctgaaaccg ctggagttaa
    29521 cacaaaccca ttgggttacg ttacacaata tccatcagtt acctccagac cagtcgcaaa
    29581 ttcaactggc aaaagcgatt ggcatcgagc agccatcact ggtccgtacg ctggaccaac
    29641 tggaagaaaa agggttaatt tcgcgtcaaa cttgtgccag cgatcgtcgg gctaaacgta
    29701 ttaaactgac ggaaaaggca gaaccactga tcagcgaaat ggaggctgtt attaacaaaa
    29761 cccgcgcgga aattttacat ggcatctccg cagaggaact ggaacaactg attaagctca
    29821 tcgcaaaact tgagcataat atcattgagt tacaggccaa agggtgaaat gaagggggcg
    29881 tgtggtcagt aacctgacca cacgcaaact tatttagcgc ggggaaacgg tcacctgact
    29941 gccattgctg gccagtacga cacgttggcc tggagagaaa tgagtgttcc cttgtttctg
    30001 taccaccatg atggtgttgc catcatcttt acgaatttcc agctcgacac cctgcgtttt
    30061 gttcattgca ctctgtacgc cctgaccggc tacaccacct gcaacagccc ctgctgcagt
    30121 agccagagaa cgcccggttc cgccgccaat agtatttccc aggaaaccac caagtacagc
    30181 accaccaatt gcgccgataa cgttggaatc atcaccgccc tgaatctgta cctgacgtac
    30241 attaacgatg gtgccatagc tgacattctg tacttgtttc gcttcagaag cggtataaac
    30301 atcccctgac agggtgtcgt tattaacaca accgacaaga gacagaccta ccattgaaac
    30361 aaccaatacg cgtttaatca ttgaaaaatc tcctgttcac catgaaacgc cacgcaagca
    30421 tccctcatgg tcaaagtata tggcatttgt gaatccatgg ttacatgttc taccaaatat
    30481 gagcaaatca taatgaaagt aggatcaacc aattataaac aaaatattca tgatgattat
    30541 tgatttgaag ataatgtttt actaccaacg gtaaaaattg gtcattccgc atggtattca
    30601 cgaaaagttt atgtttgagt ggtgcgcaaa cgtcagattg aaaaaggatg aactatgaaa
    30661 tcgggccgct ttattggcgt tatgtcaggc accagccttg atggtgttga tgttgtgttg
    30721 gcgacaattg atgaacaccg ggtcgcacag ctggcaagtt tgagctggcc gatcccggtg
    30781 tctctgaaac aggctgtact ggatatttgc cagggccagc agcttacact ttcgcagttt
    30841 ggacagcttg atactcaact cgggcgactt tttgctgatg cggtcaatgc cttgcttaag
    30901 gaacaaaacc tgcaggcgag agatatagtt gcgatcggct gtcacggtca aaccgtctgg
    30961 cacgaaccga cgggcgtggc accacacact ttacagattg gcgataacaa tcaaattgtg
    31021 gcgcgcaccg gaattacggt tgtcggtgat tttcgccgtc gcgatattgc cttgggagga
    31081 caaggtgcac cgctggtacc tgcgttccat catgcgctgc tggctcaccc aaccgagcga
    31141 cgaatggtgc tcaatattgg cggcatcgcc aatctgtcac tgctcattcc tggccagccg
    31201 gttgggggct acgataccgg tcctggtaac atgctgatgg atgcctggat ctggcgtcag
    31261 gccggtaaac cttacgataa agatgccgag tgggcacggg cgggtaaagt tattctccca
    31321 ctgctgcaaa atatgctcag cgacccatat ttctcgcaac ccgcaccgaa aagcaccgga
    31381 cgcgaatact ttaactacgg ctggctggag cgccatttgc gccattttcc gggggttgat
    31441 ccccgtgatg tgcaggccac agtggcagaa ctcaccgccg tgaccatttc tgaacaagtt
    31501 ttgttgagcg gtggctgcga acgattgatg gtatgtggtg gaggtagtcg taatccgcta
    31561 ctcatggcgc gtctggcggc attactgcca ggcacagaag tcaccaccac cgatgccgtt
    31621 ggcattagtg gcgatgacat ggaagcattg gcgttcgcct ggcttgcctg gcgaacgctg
    31681 gcgggattac caggaaatct gccttccgtc actggcgcaa gccaggagac ggtactgggg
    31741 gctattttcc ccgctaaccc gtgatatcag agttaactga atttttctta ccgcgtctgc
    31801 cgttacactt ggaacgttag ggagggcgta ttgccctcca gaccaggaaa gtcttcggga
    31861 tatgtctatg aaaaaactgt tactgatctg tttgccggtg cttctcaccg gctgtagcgc
    31921 gttcaatcaa ctggttgagc gcatgcaaac cgatacgctg gaataccagt gtgatgaaaa
    31981 accgttgacg gtcaaactga ataatccgcg ccaggaagtc agttttgttt acgataatca
    32041 actactgcat ctcaaacagg gcatttcagc gtctggtgca cgttacaccg acggaatcta
    32101 tgttttctgg tcgaaaggcg aggaagcgac tgtctataaa cgcgaccgca tcgtcttgaa
    32161 taactgtcag ttacaaaatc cgcagcgttg agatttttcc aggggcggcg cacaatagcg
    32221 tcacctactg acaatccgta aagaaaacca tgtctgataa cgacgaattg cagcaaatcg
    32281 cgcatctgcg ccgtgaatac accaaaggcg ggttacgccg ccgcgatcta cccgccgatc
    32341 cattaaccct ttttgaacgt tggctctctc aggcttgtga agccaaactg gctgacccta
    32401 ccgcgatggt ggtcgctacc gtggatgaac atgatcagcc ttatcagcgc atcgttttac
    32461 tcaaacatta cgacgaaaaa ggcatggtgt tttacaccaa cctcggcagc cgtaaagcac
    32521 atcaaatcga aaataatccg cgcgttagcc tgctgttccc ttggcatacc cttgaacgcc
    32581 aggtgatggt gatcggtaaa gcggaacgac tttcgactct cgaagtgatg aaatattttc
    32641 atagccgccc gcgtgatagc cagattgggg catgggtttc gaagcagtcc agtcgcattt
    32701 ctgcccgcgg tatccttgaa agtaaattcc tggagctgaa gcagaagttt caacagggcg
    32761 aagtgccatt gccaagtttt tggggcggtt ttcgcgtcag ccttgaacag attgagttct
    32821 ggcagggcgg tgagcatcgc ctgcatgacc gctttttgta ccagcgtgaa aatgatgcgt
    32881 ggaagattga tcgtcttgca ccctgaaaag atgcaaaaat cttgctttaa tcgctggtac
    32941 tcctgattct ggcactttat tctatgtctc tttcgcatct ggcgaaaagt cgtgtaccgg
    33001 caaaggtgca gtcgttatat acatggagat tttgatggca agcagtaact tgattaaaca
    33061 attgcaagag cgggggctgg tagcccaggt gacggacgag gaagcgttag cagagcgact
    33121 ggcgcaaggc ccgatcgcgc tctattgcgg cttcgatcct accgctgaca gcttgcattt
    33181 ggggcatctt gttccattgt tatgcctgaa acgcttccag caggcgggcc acaagccggt
    33241 tgcgctggta ggcggcgcga cgggtctgat tggcgacccg agcttcaaag ctgccgagcg
    33301 taagctgaac actgaagaaa ctgttcagga gtgggtggac aaaatccgca agcaggttgc
    33361 cccgttcctc gatttcgact gtggagaaaa ctctgctatc gcggcgaaca actatgactg
    33421 gttcggcaat atgaatgtgc tgaccttcct gcgcgatatt ggcaaacact tctccgttaa
    33481 ccagatgatc aacaaagaag cggttaagca gcgtctcaac cgtgaagatc aggggatttc
    33541 gttcactgag ttttcctaca acctgttgca gggttatgac ttcgcctgtc tgaacaaaca
    33601 gtacggtgtg gtgctgcaaa tcggtggttc tgaccagtgg ggtaacatca cttctggtat
    33661 cgacctgacc cgtcgtctgc atcagaatca ggtgtttggc ttgaccgttc cgctgatcac
    33721 caaagcagac ggcaccaaat ttggtaaaac tgaaggcggc gcagtctggt tggatccgaa
    33781 gaaaaccagc ccgtacaaat tctaccagtt ctggatcaac actgcggatg ccgacgttta
    33841 ccgcttcctg aagttcttca ccttcatgag cattgaagag atcaacgccc tggaagaaga
    33901 agataaaaac agcggtaaag caccgcgcgc ccagtatgtg cttgcagaac aggtgacacg
    33961 tctggtgcat ggtgaagaag gtttgcaggc ggcaaaacgt attaccgaat gcctgttcag
    34021 cggttccttg agtgcgctga gtgaagcgga cttcgaacag ctggcgcagg acggcgtacc
    34081 gatggttgag atggaaaagg gcgcagacct gatgcaggcg ctggtcgatt ctgaactgca
    34141 accttcccgt ggtcaggcgc gtaaaactat cgcctccaat gccatcacta ttaacggtga
    34201 aaaacagtcc gatcctgaat acttctttaa agaagaagat cgtctgtttg gtcgttttac
    34261 cttactgcgt cgcggcaaaa agaattactg tctgatttgc tggaaataat gcattaagtg
    34321 gaaaggggga gtgagaaatc actccccctg gtttttatac agggaacatg atgaaaaata
    34381 ttctcgctat ccagtctcac gttgtttatg gtcatgcggg taacagtgcg gcagagtttc
    34441 cgatgcgccg cctgggcgcg aacgtctggc cgctgaacac cgttcaattt tctaatcaca
    34501 cccaatacgg caaatggacc ggttgcgtga tgccgcccag ccatttaacc gaaattgtgc
    34561 aaggcattgc cgccattgat aaattacaca cctgtgatgc cgtattaagt ggctatctgg
    34621 gatcggcgga gcagggtgaa catatcctcg gtatcgttcg tcaggtgaaa gcagcgaatc
    34681 cacaggcgaa atatttttgc gatccggtaa tgggtcatcc ggaaaaaggc tgtatcgttg
    34741 caccgggtgt cgcagagttt catgtgcggc acggtttgcc tgccagcgat atcattgcgc
    34801 cgaatctggt tgagctggaa atactctgtg agcatccggt aaataacgtc gaagaagcgg
    34861 ttctggcagc gcgcgaactc attgcgcaag ggccacaaat tgtgttggtt aaacacctgg
    34921 cgcgagctgg ctacagccgt gaccgttttg aaatgctgct agtcaccgcc gatgaagcct
    34981 ggcatatcag ccgtccgctg gtggattttg gtatgcgcca gccggtaggt gttggtgatg
    35041 tgacgagcgg cttactgctg gtgaaactgc ttcagggggc aacgctgcag gaggccctgg
    35101 aacatgtgac cgctgcagta tacgaaatca tggtgaccac aaaagcaatg caggaatatg
    35161 agctgcaagt ggttgctgct caggatcgta ttgccaatcc agaacattat ttcagcgcaa
    35221 caaagctctg aaatatctca tttcggccca caacgacctg tgggccgatg gctattactt
    35281 taagccttcc gctgacagcg cgtcttgtac ttccggacgt tcagccatac gttgcataaa
    35341 tgccgcaatg tgctctaacc cttccagatt cagtttcacc gcgtatgccc agcgcagaac
    35401 cgtaaacaga taggcatcag caattgtaaa tcgttgcccg cagatccagt gctcatcctt
    35461 cagtgcctcg ttcacatatt gcagcttctt ctccagctgc gcgcgaactg tcgatttgta
    35521 ctcttccggt gtatcagggc gaaacagagg tgtgaaacct ttatgcagct cggtggcgat
    35581 gtaattcagc cattcgatgg ttttatagcg ggaaatactg tttaccggtg ccagcaactg
    35641 gcggtcgggg acgctgtcgg caagatactg cataatcgct acgccttccg tcagcaaagt
    35701 accatcgtcc agcagcaatg caggtacctg tcccttaggg ttaacggcaa agtaatcgtc
    35761 accgttttcg agacgttttt tcattaaatc cacactgacg agcgtaaaat cctttccgct
    35821 ctcacgcagg gtgatatggg aagcgagaga gcaggcgccc ggtttgtaga acaatttcat
    35881 cggtaactcc ttttgtctgt ggtttaaagt atggtagtgc gttgctgaac aaaaaaaaag
    35941 ccgctaacga aaagttagcg gcttgtaaaa gagtttccct gaattttacg ctacggctgc
    36001 tttcgccgct ttgtctgcag cgtcatcctg cgtcatgcgg tgcagtttcg gcgcggtaaa
    36061 cagcatcagt actgcaataa cggcagtagc gacaccaatc tgcaagaata cgcgaccata
    36121 gacttccagt gacatcagcg gatcggtaac gttatccggc acagccatca tacctgcaac
    36181 ataaccacca atcaggtttg caccggcagt ggtcaggaac cagctaccca taatgaagcc
    36241 catcagacgc tgcggaacga gttgtgcaac cattgccaga cccaggccag agatcatcag
    36301 ttccccgata ctctgcaggc catagctcgc gaccagccag cttacagaca cgataccagc
    36361 gtcagacgcg aatttcgcac ccagcggcag aatcaggaac gcaccagaac acatcaccat
    36421 gccgatagca aacttggttg gcattggcag ggtatcgccc atcttgttat agatagcggc
    36481 cagaatcggg ctaccaatga tgatccagaa cgggttcagt gcctgatact gctcaggttc
    36541 tacggccagg cccagaatgg tgtgttcaac gttacgaatc gcaaagaagt tcagtgacgt
    36601 tggcatctgg ctataaagca cgaagaagat aatggcttcg agcatcagga tgaaggcaac
    36661 gatcatttta cgacgcgcag cacctttcat cgcgaaggct tctttaccga agataaccac
    36721 gataccgaag gcaacaacgc ccagcgccat acgcgcaact tcctgattgt gcagcagcca
    36781 ggtggcgata gcgatcagtg ccacaacacc aataatggtc agcagcaggt tacggtagtt
    36841 gataggctcg aagtctggtt ttgaaccgta ctgtttaacc caacgttggc agaaggcgaa
    36901 gttaacgata gtgatcagca ggcctacaac gctcaacgca aacgcaacac tccagccgta
    36961 tttcgcggcc agccacggcg tagcaatcat agagaagaaa gagccgatgt tgacggacat
    37021 gtagtacatg gtgaatgcac cgtccagacg cgggtcgttt ttctcatagc atgtagaaag
    37081 cagagaagac gggttagctt taaacaggcc gttaccgacc gcaatagccg ccatacccat
    37141 ataaacgata ccggcgtcgt gaccagacca ggcaaccaga gcataaccaa tcgccagcac
    37201 aatagcgccg agcataatta cgcgtttagt acccagtacc ttgtcaccta accagccgcc
    37261 gatagcgacc agaccataaa ccagggcact aaaggaagag aaaagggtga ttgaatccgc
    37321 ttcagacata cccagttgtt taaccaggta aacagccata attccttgta ggccgtaata
    37381 accaaaacgt tcccataact caatcgagaa gatgagatag aacgccttcg gttgtttaaa
    37441 agcgttcaaa ctgacgcttt cagttggttt ttggtttgca gtggacactt ttacctcttt
    37501 ttttacatcc catattaacg ggggtgcttt ctcgcgttat gcctaaaaaa catacgcgca
    37561 attgttatag tgggagggga aacggcaggt aatgttcact atcttgcact ttcagacaag
    37621 tcctttgtaa taatctgtta catataactg aggcggggaa ttcaaccatt ccgggaaatg
    37681 ttatccagcg ttaaattttt caaaaaacgt aacggcagat ttt
//
LOCUS       DADSZD010000026        36398 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.026, whole genome shotgun sequence.
ACCESSION   DADSZD010000026 DADSZD010000000
VERSION     DADSZD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.026"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            267..1640
                     /gene="aroP"
                     /locus_tag="J1Z28_001200"
     CDS             267..1640
                     /gene="aroP"
                     /locus_tag="J1Z28_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBA7716047.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYRYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGAWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1683..2537)
                     /gene="ampE"
                     /locus_tag="J1Z28_001201"
     CDS             complement(1683..2537)
                     /gene="ampE"
                     /locus_tag="J1Z28_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBA7716048.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDGVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2534..3085)
                     /gene="ampD"
                     /locus_tag="J1Z28_001202"
     CDS             complement(2534..3085)
                     /gene="ampD"
                     /locus_tag="J1Z28_001202"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBA7716049.1"
                     /translation="MLLEQEWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3173..4066
                     /gene="nadC"
                     /locus_tag="J1Z28_001203"
     CDS             3173..4066
                     /gene="nadC"
                     /locus_tag="J1Z28_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBA7716050.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANH
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFC"
     gene            4269..4709
                     /gene="ppdD"
                     /locus_tag="J1Z28_001204"
     CDS             4269..4709
                     /gene="ppdD"
                     /locus_tag="J1Z28_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBA7716051.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4719..6104
                     /gene="gspE"
                     /locus_tag="J1Z28_001205"
     CDS             4719..6104
                     /gene="gspE"
                     /locus_tag="J1Z28_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HBA7716052.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHQVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQEEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLEMHARQAGMHTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6094..7296
                     /gene="hofC"
                     /locus_tag="J1Z28_001206"
     CDS             6094..7296
                     /gene="hofC"
                     /locus_tag="J1Z28_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBA7716053.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7331..8374)
                     /gene="guaC"
                     /locus_tag="J1Z28_001207"
     CDS             complement(7331..8374)
                     /gene="guaC"
                     /locus_tag="J1Z28_001207"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBA7716054.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREEWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            8599..9219
                     /gene="coaE"
                     /locus_tag="J1Z28_001208"
     CDS             8599..9219
                     /gene="coaE"
                     /locus_tag="J1Z28_001208"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBA7716055.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9219..9962
                     /gene="zapD"
                     /locus_tag="J1Z28_001209"
     CDS             9219..9962
                     /gene="zapD"
                     /locus_tag="J1Z28_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBA7716056.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9972..10169
                     /gene="yacG"
                     /locus_tag="J1Z28_001210"
     CDS             9972..10169
                     /gene="yacG"
                     /locus_tag="J1Z28_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBA7716057.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10384..10773)
                     /gene="mutT"
                     /locus_tag="J1Z28_001211"
     CDS             complement(10384..10773)
                     /gene="mutT"
                     /locus_tag="J1Z28_001211"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBA7716058.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMSE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     KWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10833..13538)
                     /gene="secA"
                     /locus_tag="J1Z28_001212"
     CDS             complement(10833..13538)
                     /gene="secA"
                     /locus_tag="J1Z28_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBA7716059.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13600..14112)
                     /gene="secM"
                     /locus_tag="J1Z28_001213"
     CDS             complement(13600..14112)
                     /gene="secM"
                     /locus_tag="J1Z28_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBA7716060.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14343..15260)
                     /gene="lpxC"
                     /locus_tag="J1Z28_001214"
     CDS             complement(14343..15260)
                     /gene="lpxC"
                     /locus_tag="J1Z28_001214"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBA7716061.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15361..16512)
                     /gene="ftsZ"
                     /locus_tag="J1Z28_001215"
     CDS             complement(15361..16512)
                     /gene="ftsZ"
                     /locus_tag="J1Z28_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBA7716062.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16573..17835)
                     /gene="ftsA"
                     /locus_tag="J1Z28_001216"
     CDS             complement(16573..17835)
                     /gene="ftsA"
                     /locus_tag="J1Z28_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017374018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBA7716063.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17832..18662)
                     /gene="ftsQ"
                     /locus_tag="J1Z28_001217"
     CDS             complement(17832..18662)
                     /gene="ftsQ"
                     /locus_tag="J1Z28_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBA7716064.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGMRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18664..19584)
                     /locus_tag="J1Z28_001218"
     CDS             complement(18664..19584)
                     /locus_tag="J1Z28_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBA7716065.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19577..21052)
                     /gene="murC"
                     /locus_tag="J1Z28_001219"
     CDS             complement(19577..21052)
                     /gene="murC"
                     /locus_tag="J1Z28_001219"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBA7716066.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21106..22173)
                     /gene="murG"
                     /locus_tag="J1Z28_001220"
     CDS             complement(21106..22173)
                     /gene="murG"
                     /locus_tag="J1Z28_001220"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBA7716067.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22170..23414)
                     /gene="ftsW"
                     /locus_tag="J1Z28_001221"
     CDS             complement(22170..23414)
                     /gene="ftsW"
                     /locus_tag="J1Z28_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBA7716068.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23414..24730)
                     /gene="murD"
                     /locus_tag="J1Z28_001222"
     CDS             complement(23414..24730)
                     /gene="murD"
                     /locus_tag="J1Z28_001222"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBA7716069.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24733..25815)
                     /gene="mraY"
                     /locus_tag="J1Z28_001223"
     CDS             complement(24733..25815)
                     /gene="mraY"
                     /locus_tag="J1Z28_001223"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBA7716070.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25809..27167)
                     /gene="murF"
                     /locus_tag="J1Z28_001224"
     CDS             complement(25809..27167)
                     /gene="murF"
                     /locus_tag="J1Z28_001224"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBA7716071.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LSGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27164..28651)
                     /gene="murE"
                     /locus_tag="J1Z28_001225"
     CDS             complement(27164..28651)
                     /gene="murE"
                     /locus_tag="J1Z28_001225"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBA7716072.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGHRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28638..30404)
                     /locus_tag="J1Z28_001226"
     CDS             complement(28638..30404)
                     /locus_tag="J1Z28_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBA7716073.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30420..30785)
                     /gene="ftsL"
                     /locus_tag="J1Z28_001227"
     CDS             complement(30420..30785)
                     /gene="ftsL"
                     /locus_tag="J1Z28_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBA7716074.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30782..31723)
                     /gene="rsmH"
                     /locus_tag="J1Z28_001228"
     CDS             complement(30782..31723)
                     /gene="rsmH"
                     /locus_tag="J1Z28_001228"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBA7716075.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31725..32183)
                     /gene="mraZ"
                     /locus_tag="J1Z28_001229"
     CDS             complement(31725..32183)
                     /gene="mraZ"
                     /locus_tag="J1Z28_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBA7716076.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32785..33789)
                     /gene="cra"
                     /locus_tag="J1Z28_001230"
     CDS             complement(32785..33789)
                     /gene="cra"
                     /locus_tag="J1Z28_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBA7716077.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33871..33957)
                     /locus_tag="J1Z28_001231"
     CDS             complement(33871..33957)
                     /locus_tag="J1Z28_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBA7716078.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33969..34460)
                     /gene="ilvN"
                     /locus_tag="J1Z28_001232"
     CDS             complement(33969..34460)
                     /gene="ilvN"
                     /locus_tag="J1Z28_001232"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBA7716079.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34463..36187)
                     /gene="ilvI"
                     /locus_tag="J1Z28_001233"
     CDS             complement(34463..36187)
                     /gene="ilvI"
                     /locus_tag="J1Z28_001233"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBA7716080.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTHGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
BASE COUNT         8756 a        10188 c         8894 g         8560 t
ORIGIN      
        1 tcatatgtaa tgaatttata ttcattgtat atattgaggg gatttgaggc tgacctttac
       61 aaacggttcc tttttaagca actcatcttc aaccatgcat aaagcgggtg cattcgctgc
      121 ggcataccat tattcttgat ctgacggaag tccttttgta acaattcaaa cttccttgat
      181 gtaaacaaat caatacaaca aacggaatta caaaattaca catgcattgc tgcgtagttc
      241 aaaaaaacaa cacaacacga ggtttcatga tggaaggtca acagcacggc gagcagctaa
      301 agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg atagggacag
      361 ggctattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt atcctgggtt
      421 acgccattgc tggttttatc gcctttctga tcatgcgtca gctaggtgaa atggtggtcg
      481 aagaacctgt cgcaggctcc tttagccact ttgcttatag atactggggc agttttgccg
      541 gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg gcagagctga
      601 ctgccgtggg taaatacatt cagttctggt atccggaaat cccaacctgg gtttctgccg
      661 ccgtgttctt tgtggtgatc aacgccatca acctgacaaa cgtaaaagtg tttggcgaga
      721 tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc atcttcggcg
      781 cctggctgct attcagcggc aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc
      841 agggtggttt cctgccacac ggcttcaccg gtctggtgat gatgatggcg attatcatgt
      901 tctctttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat aacccggagc
      961 aaagtatccc aaaagcaact aaccaggtta tctaccgcat cctgattttc tatattggtt
     1021 cgttagccgt tctgctctca ctaatgccgt ggacccgcgt taccgccgat accagtccgt
     1081 ttgtgctgat cttccacgag ttaggcgata cctttgtagc gaatgcgctg aatatcgtgg
     1141 tactgactgc tgcgctctcc gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt
     1201 tcggtctggc acaacaaggg aacgcgccaa aagcgctggc gtctgtcgat aaacgcggcg
     1261 taccggttaa cactattctg gtgtctgcgc tggttacggc attgtgcgta ctgattaact
     1321 accttgcccc agagtccgct ttcggactgt taatggcgct ggtggtatct gcactggtca
     1381 tcaactgggc gatgatcagt ctggcgcata tgaaattccg tcgtgccaaa caagaacaag
     1441 gcgtggtaac tcgcttccct gccctgcttt atccgctggg taactggatc tgcctgctgt
     1501 ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt tcggtatacc
     1561 tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag aaaaccgcca
     1621 aagccgtaaa agcgcattaa tctctctacg ccctcacccg tgcagggtga gggcaataat
     1681 ctttacacca acgccccgta aatcgtcagt agcgcaatta ccaccacgac cacgaacgag
     1741 gttttcttcg ccattgaaac cgctgccttt ggcgtctcca ccttatcgac atgcggttca
     1801 cgcgccagag agaactgtgc cagacgcgtt aatacctgat attgcgaagt atggaaatca
     1861 cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa ggcatatacc
     1921 acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacgccatc aatgccggac
     1981 tgtaaacgat gatgcggcgt ctggtatcgc gccagccagt attgccatgc acgcaaaaac
     2041 gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca gaacagcgga
     2101 gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg caaatactca
     2161 cgttcatcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc catcgtggca
     2221 cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg aagacgaact
     2281 ttacctgcgc caatacacag caaaccaatc agcagccaca ccagcagcgt gggaacgttg
     2341 aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc aataatggtc
     2401 atgcctaatg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga
     2461 tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa caccagtaag
     2521 gttgtaaata gcgtcatgtt gtctccttgc tgaccagcgc acgaaaccgt gcccaatcaa
     2581 atgcagggcc gggatcggtt ttccgatccg gcgcaatatc acaatggccc gtcatgttat
     2641 tagcgatatc cggatagcga tcaatcagtg cccgcgtaac cgccgcaagc tgttgatact
     2701 gcgcatcggt atacgccagc gtatcggtgc cttccagctc aatcccaata gaaaaatcat
     2761 tgcagcgttc acgcccctga tactgagaga ctcccgcatg ccaggcacgt ttatcgaaag
     2821 gaacatactg gactatttca ccatcgcggc gaatcaaaca gtgagcggag acgcgcaaat
     2881 gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg aataatgcgt
     2941 cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc accagcaggg
     3001 tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa
     3061 ccagccactc ctgttctaac aacatgcagg atctccttat atgtggtgct aatacccggt
     3121 tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta acatgccgcc
     3181 tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta atctcgatat
     3241 ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg atgccaacaa
     3301 tgacattacg gcaaaacttt taccggaaaa ctctcgctct catgccacag tgatcacccg
     3361 cgagaatggc gtattttgcg gcaaacgctg ggttgaagag gtgtttattc aactggcagg
     3421 cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaacg ccaatcaccc
     3481 cttgttcgaa cttgaaggcc catctcgcgt gctgttaacg ggcgaacgca ctgcgcttaa
     3541 ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg aattactgga
     3601 aggcaccaac acgcagctgt tggatacgcg caaaacctta cccggcctgc gttcagctct
     3661 gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt
     3721 cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg tcgaaaaagc
     3781 gtcctggttg cacccggatg cgccagtaga agtcgaagta gagaatctgg aagaacttga
     3841 tgaagccctg aaagcaggtg ccgatatcat catgctggat aacttcgaaa cagaacagat
     3901 gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac
     3961 tgacaaaaca ctgcgcgaat ttgccgaaac gggcgtggac tttatctccg tcggtgcgct
     4021 aactaaacac gtacaggcac tcgacctatc aatgcgtttt tgctgattgc tgacagattt
     4081 atccgctcca tcgcgggcgg ataatctccc ctttcccaac tttcttcgta acgcctcgca
     4141 aatttcttgt ctttcgctgc aacgaaataa aaaactccgg aagctgcttt cccgtttcgt
     4201 tttttgcccc tcgcttgtgc tgatggcgtt tgccaaagta gcgccaacta aatcaaggag
     4261 cgaaacaaat ggacaagcaa cgcggtttta cacttatcga actgatggtg gttattggca
     4321 tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg cgcaaagccg
     4381 cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc
     4441 tggaacatgg tggattaaat acctgcgacg gtggcagcaa tggcattcct tcgcctacca
     4501 ccacccgcta tgtttcagcc atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag
     4561 aaagtctcaa tgggctaagc gtcgtcatga caccgggttg ggataacgca aacggcgtca
     4621 ccggctggac gcgtaactgc aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag
     4681 atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca cagctcaccg
     4741 ccctgtgtct gcgttatcag ggagtcttgc tggatgccag cgaagaggtg gttcatgttg
     4801 cggtagtcga tgcaccttcg catgagctac tggacgcatt gcatttcgct accaccaaac
     4861 gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt cgcacacaac
     4921 agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg ctgactcgaa
     4981 cgttacagtc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca gcggacaatg
     5041 cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggac gtttcaccgg
     5101 atgccggagt cgcattaacc gccagattaa aagtgctggg aaatctggat attgcggaac
     5161 atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc gtctcatttc
     5221 gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg ttacagcagg
     5281 tgagccaggc actggatgtt aacacgctgg gaatgcagcc gttacaactg gcggactttg
     5341 ctcatgcatt gcaacaacca cagggattgg tgctggtaac tggccctaca ggcagcggca
     5401 aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt aatatttgta
     5461 gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa atccatccgc
     5521 gtgccggact cacctttcag ggcgttttgc gtgcgttatt gcgccaggac cctgacgtca
     5581 tcatgatcgg agagatccgc gatggcgaaa cggcagaaat tgccataaaa gccgcgcaaa
     5641 ccggtcacca ggtgttgtcc acgctacaca ccaattccac ctgcgaaacg ctggtgcgtt
     5701 tacagcaaat gggcgttgct cgctggatgc tctcatcggc acttacgctg gtaatagccc
     5761 agcgtctggt acgtaaactt tgcccacatt gtcgccgaca gcaagaggag cccatccata
     5821 ttccagacaa tgtatggccg tcgccgctgc cccactggca ggcacccggt tgtgtacatt
     5881 gctaccacgg tttttatggt cgcacggcct tatttgaagt tctgcccata acaccggtca
     5941 tacgtcagct tatttccgct aataccgacg ttgaatcgct ggaaatgcac gcccgacagg
     6001 cgggtatgca tacgcttttt gaaaacggct gcctggccgt ggagcaaggc ttaaccacct
     6061 ttgaagagtt aatccgcgta ttggggatgc cgcatggcga gtaagcaact ctggcgctgg
     6121 catggcataa ccggcgacgg taatgcgcaa gatgggatgc tatgggcaga gagtcgtgct
     6181 ttactgctta tggcactaca gcaacagatg gttaccccac ttagcctgaa gcgaatcgcc
     6241 atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca actggcgacg
     6301 ctactcaaag ccgggttaac gctttctgaa gggctggctc tgctggcgga acagcatccc
     6361 agtaagcagt ggcaagcgtt gctgcaatca ctggcgcacg atcttgaaca gggcgttgcc
     6421 ttttccaatg ccttattacc ctggtcagag gtatttccgc cgctctatca ggcgatgatc
     6481 cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc gcgtcagcaa
     6541 aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc cattatcatt
     6601 ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgtactgcc ggagtttgcc
     6661 gctatctata agacctttaa cactccgcta ccggcgctaa cgcaggggat catgacgctg
     6721 gcaagtttta gtggcgaatg gggttggttg ctggtgctat tcggctttct gctggcgata
     6781 gccaataagc tgctgatgcg ccacccaacc aggcttatcg cacggcagaa attgctgtta
     6841 cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat ctttacgatt
     6901 ctgacactga cacaaagtgc aggcatttct tttttacagg gcgttgagag cgtcagagaa
     6961 acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga tatcagtaac
     7021 ggtcacccca tctggctggc gctaaaaaat gccggtgagt ttagcccgct ctgtttgcaa
     7081 ttagtgagaa caggagaggc atccggctcg ctggatctca tgttagacaa cctcgcccat
     7141 catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact ggaaccggcg
     7201 ttgctgatca taacgggagg aattatcggt acgctggtgg tggcaatgta tctgccaatt
     7261 ttccatttag gcgatgcgat gagtggaatg ggataacgct ggcgtgttgc tccacgccag
     7321 cgttgggaga ttacaggttg ttgaagatgc ggttttcttg ttccagcaca cggataaatg
     7381 tggtgcgctt ggtcagctct ttcaggcgtg aagccccaac gtatgtacaa gctgaacgca
     7441 ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc agcttaacgg
     7501 ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc atcgcagact
     7561 cggagctcat gccgtagaac agcataaatt tctcgccatt ctcctcaacg atgcgaccgc
     7621 cgctctcttc gtggcccgcc agcataccgc caagcatgac gaaatcggca ccgccgccaa
     7681 aggctttcgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc attccgccca
     7741 gaccgtgcgc agcatcggca cattcgatca ccgcagaaag ttgcggataa ccgacgcctg
     7801 ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg atatcagcac
     7861 cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca caaatggttt
     7921 tggtcggcca ctcttcacgc gctttcgcaa cgaactgcac gaagtgttcg gaataaccat
     7981 tcgccacgtc aatacaaacg aaatttaatg ccgggttcag gtcgagaatc tgtttagttt
     8041 tttcgaaatc cgcatctgat gtaccggtag aaaccatcac atgtttcagc acatcagcgg
     8101 aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc acagcagtca
     8161 gaatatcaaa agaagccagc gcagaggcca tagaaaatgt gcctacggtg tccatatttg
     8221 cggcgataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg aattgacgtt
     8281 ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg agaacgtctt
     8341 taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg ttaatggcta
     8401 taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg cggcgcaaga
     8461 ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa atagtgagcg
     8521 aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa tcatgatgaa
     8581 ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat tggcagtggc
     8641 aagagtaccg ttgccaatgc gtttgcggat ctcggaatta acgtcattga tgccgatatt
     8701 attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc tgatcacttt
     8761 ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg cgagcggatc
     8821 ttcgccaacc cggaagagaa aaactggctt aacgacctgc tgcatccgct gattcagcaa
     8881 gagacgcaac accagatcca gcaagctact tccccctatg tactgtgggt tgtgccattg
     8941 ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga tgtcagccca
     9001 gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca tgtcgaacaa
     9061 atccttgctg ctcaggcaac gcgcgaagct cgccttgccg tggcagatga cgtcattgat
     9121 aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc acactatttg
     9181 cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag gtcctttttg
     9241 aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg attcagcaac
     9301 tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc cgtaatgtca
     9361 gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg aaagaacttg
     9421 accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac cagagccgta
     9481 ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc gcgccgcgta
     9541 tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt acgtcagcga ctgagcatcc
     9601 caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat ctaccccagg
     9661 cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc acccaggcac
     9721 ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa accagcctga
     9781 atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat ctgtcgctcg
     9841 attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt cgttttatgc
     9901 cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg gcctgttgct
     9961 aaggagtaaa aatgtcagaa actattacgg tgaattgccc aacctgcggg aaaacggtgg
    10021 tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag ctgatcgacc
    10081 tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt tccgaaagcg
    10141 atgactggag cgaagagcca aagcagtgac attctatctg atgcgacgct ggcctgataa
    10201 aacgcggaca gcgtcgcatc aggcatcttg tgcaaatgtc ggatgcggcg tgaacgcctt
    10261 atccgaccta taaaaacaaa tttatcaatg agttagaaat ccctgtaggc ctgataagac
    10321 gcgccagcgt cgcatcaagc atcgtgtgcg aatgtcggat gcggcgaaaa cgtcttatct
    10381 gacctacaga cgtttaagct tcgcaattac cggttcattg gctggcggaa aatcatcggc
    10441 attaagaccg accagcgaca tccacttacc gggttgccct tctttacccc agggctcccc
    10501 ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga attcatattc
    10561 cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acctcttcct gaagttcacg
    10621 caccaccgcc tgttccggcg tttcgctcat ttcaatttta ccgccgggaa actccagttt
    10681 attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt tctcgttgcg
    10741 aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa aaaggcgcag
    10801 aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg gcactgcttg
    10861 tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg ctcaccggtt
    10921 tgcgccgcca gtgcagctgc agctgcagag tcgtcatcct gatggctaag ctgctgcatt
    10981 tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac ctcttcaggc
    11041 atacgtacct gaactttgct cagcgtactg ataacttcat attttaacga ctccagcatc
    11101 gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt ctgtgcatag
    11161 ccacgcaggt ggataccctg acgcagatag tccatcgctg ccaggtgctc tttccacagg
    11221 gagtcaagcg tttgcagcat gacgcctttc tcgaagtgac gcatcatctc tgcaccaacc
    11281 acttcttctt tacgctgata cacttcgatg gactgcgcca gaatgcgctc acgcagcgtc
    11341 tcttcatgca gttctggttc tttatccagc cactcagcaa ttggtaaatc gaggtcgaaa
    11401 tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccaa cgactgtggc
    11461 ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt gatggtttcg
    11521 ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg acgctgatcg
    11581 ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg actttcaact
    11641 ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc ttcgcctggc
    11701 ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat acgcatcaac
    11761 gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg accagaacga
    11821 ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat gatatgcagg
    11881 ccacctgctg ccagtaccgc atcgtgacgt acctgccagt cggctttaat tttttcaatt
    11941 tgctctgcag tcggattttc cagcgcggca acttctgcct gccagctacc accgagcaca
    12001 atatcggtac cacgacccgc catgttggtc gcgatagtca ccgcagccgg ataacctgcc
    12061 tgagcaacaa tcgccgcttc gttggcatgg aatttggcgt tcaggacgtt gtgcttaata
    12121 ccggctttgg tcagttcatt tgacaccagc tccgattttt cgatggagat tgtacccacc
    12181 agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc ctgaattttt
    12241 tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg acggttggtc
    12301 ggaacaacga cagtatccag cttatagatg gagctgaatt cgaaagcttc ggtatcagca
    12361 gtaccagtca tccccgccag tttttcatac agacggaagt agttctggaa tgtgatcgaa
    12421 gccagcgtct ggttttcgtt ctggatctgc acaccttctt tcgcttccac agcctggtgc
    12481 agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc aacgatgata
    12541 acttcaccat ctttaacgat gtagtcgacg tcacgggtaa acagcgcatg agcgcgcagc
    12601 gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga ctccccttca
    12661 tccatgatgc cttctttaac cagcagttct tcaatcagaa ccagaccacg ttcggtcagg
    12721 ttcacctggc gagatttttc atccaccgag aagtggcctt caccctggaa ggtttcggag
    12781 tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt atacatttcc
    12841 gagctgtctt ctgctgggcc ggaaatgatc agcggtgtac gcgcttcatc gatgaggatg
    12901 gagtccactt cgtccaccag cgcatagtgc agtttacgtt gtacacgttc ttcagggctg
    12961 aatgccatgt tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc gtaagtgatg
    13021 tcagctgcat aagcttcacg ctttgccggt gctggcatgc ccggcaggtt gataccaaca
    13081 gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg cgccaggtag
    13141 tcgttgacgg taactacatg cacgccttta ccggttagtg cgttcaggta agcaggcagc
    13201 gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg ttcgttaaga
    13261 accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg cttacttgcc
    13321 tcacgcacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc tttttccaga
    13381 cgcgcacgaa actctgcggt tttccctttc agttcttcat cggagagttt ttccatctcc
    13441 ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt gcgatcgtta
    13501 cgactaccga aaactttagt taataatttg attagcataa taaaatctca aacgccccgc
    13561 gttgcggagt taataaaatg aagtaaaggt ttattgttgt tagctgaggc gttgagggcc
    13621 agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg cttgtggggt
    13681 aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct gggtcaacag
    13741 cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag attcttcagc
    13801 aacgggcagc gtttgcggtg ccattgcgaa ggaaagatga cggattaccg tgcgaatggc
    13861 atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgcgtgt tcgcttccag
    13921 caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg ttgtcgcttt
    13981 tgcgggcgca tttggttcgg cggcgttgct gagcgcaggc aaacctaaac tcgccgcgac
    14041 catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc agcgcgtcag
    14101 tattccactc acgttattgc catcccgatc gaattcaaac gtatttagcg cacaaatctt
    14161 atcattaaag cctgaagtcc acagcacgaa tgaagataag ttgtgcgtaa aagtgcttaa
    14221 gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa aacgactggt
    14281 tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca gtataaatgt
    14341 cgttatgcca gtacggctga aggcgctttg aaggccaacg gcagttctgc gtcatcctgg
    14401 aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag tttgttattc
    14461 agtgcatgac cagatttata agcggtaaat gcaccaataa tattgtgacc acacatgaac
    14521 aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa acgcaggccg
    14581 tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct gccgcccagg
    14641 cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt acgcgcacgg
    14701 ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg gttgctggaa
    14761 tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt gtacggctta
    14821 aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttaatgcg aacaaatttc
    14881 ttagcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa cggagcggcg
    14941 ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt atcgatgccc
    15001 aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgcacatc atgctcgttg
    15061 accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg gaaatctacc
    15121 ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc agggcgtaac
    15181 gtcagggtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac gatacgttta
    15241 agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac cgaagtgtac
    15301 tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc cagtcaattc
    15361 ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct ctttcgcagt
    15421 ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg tcagcggagc
    15481 catcccgtgc tgctggtagc gatccatcac tggctgctga acctgcttat tggtcaccag
    15541 agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacagtta cgcgcagctc
    15601 gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt cggaagcaaa
    15661 tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga agcccgccgt
    15721 gatgttaacc agcacgccgc gtgcgccaga caggtcgata tcttccagca gcggagaaga
    15781 gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc cagaacccat
    15841 cattgcgtag cccatttcag acattacggt gcgtacgtct gcaaagtcca cgttcatcaa
    15901 acccggacga gtaatcagtt cggcgatacc ttgcacagcg cctttgagta catcgttcgc
    15961 tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca gtttgtcgtt
    16021 cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct gctccgcgaa
    16081 tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa cggtcaggat
    16141 acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg taccaccacc
    16201 catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca gtgcatcgcg
    16261 atcctcatca gccgcattgc ggccaacttc tggattagcg ccagcgccca gtcctttggt
    16321 gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg cttgtgcatc
    16381 ggtatttacc gcgaagaatt caacaccttc aatgcgctca cgcaccatgt gttcaacagc
    16441 attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg taagttccat
    16501 tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta atcatcgtcg
    16561 gcctcataaa aattaaaact cttttcgcag ccaactattg agtcgcttga tccacgagcc
    16621 aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg actctttccc
    16681 atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat ccgttaaacc
    16741 ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct gagcacaggc
    16801 tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg ccaggtgatg
    16861 tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt tgaccagatt
    16921 gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt gcagactccg
    16981 cggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa cgatggagcc
    17041 cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtca cttggcggtg tgccaaaggc
    17101 gtaagcgata tcactggtca cgacattgcc agcataagga attaccttcg tgtggcgcaa
    17161 tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga caacgcagac
    17221 acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc cggcaaatat
    17281 cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt tcgccatatc
    17341 gttgtgacat gtgatcaggt gcacttttgc ctgcatacgc acgcccgaaa gtcctaccgg
    17401 attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat gcagcacacg
    17461 atgctcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca catcttcttg
    17521 cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc tgatgtgctt
    17581 accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt ctgcctggtc
    17641 aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc ctttatccat
    17701 accacgcgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg gcagaacttc
    17761 ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca gttttctgtc
    17821 cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt agattcctct
    17881 ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc aacgtagcta
    17941 atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc tacaaagcga
    18001 gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt attcagcgtc
    18061 aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct gtcctttgcc
    18121 agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc ttccgggcca
    18181 tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa agtgtttcct
    18241 tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc aaccagatga
    18301 atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca cggcaggcgt
    18361 tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc cggctcaccc
    18421 aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc accggtcaac
    18481 accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac gacccagccg
    18541 ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag acgcattcca
    18601 ttattgcggc gagaagaaac ctcttcttcg ctgtttcgcg tgttcagagc agcctgcgac
    18661 atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca tacctgcctg
    18721 acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat tggcttccag
    18781 cagataaaac tgtccatcac tgtccagcat aacgtcaata cgtccccatc ctttgcaacc
    18841 taacgtcgtc catgctttta gcactaatgc ctgcaaattg gcctcttgtg acgcttccag
    18901 acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat aatcatagaa
    18961 ggttccggcc ggttgaatac gtattgacgg taaaatttct tcaccgagta tcgcaaccgt
    19021 gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct gaaatgccaa
    19081 tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca cactggaacc
    19141 ttcgcggctc ggcttaacga taaccggcaa acccagagaa gaaatttctg ctaactgctt
    19201 atcgttaagg cctttttcaa actctgcgcg ggttaacgct acccacggcg cgaccggtaa
    19261 tccggcaccc tgccacagaa gtttgctgcg tagtttatcc attgaaagcg cagatgccat
    19321 cactccgctg ccggtataag gcaagcccat cagctcgagc attccctgca gcgtaccatc
    19381 ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg acttcagttg
    19441 cgtcacgtcg acttctttcg ggtcgacagg atacgcgtca ataccgcctt cacgcagccc
    19501 ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggagg tcccgcccaa
    19561 caggaccgcg attttatcag tcatgttgtt cttcctccgg agtttgcggc ttcagtttga
    19621 tttcagctaa agaacgagca atttttccga tattaccagc cccctgaacg agaatcaggt
    19681 cgttaccggt taataccggt gccagcatct cggctacctg cgccgggtcc ggcaccagaa
    19741 tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg tccgctcccg
    19801 gaattggcgc ttcaccagcc ggatacactt ccagcatcaa cagggtatca acctgcgtca
    19861 gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc ggctgaaaca
    19921 gcattaccag gtttttatcc ggccagcctg cgcgcgccgc tttaatagtg gcgtccactt
    19981 ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgcttttt ccattcactg
    20041 gctccagcgg gaattcaccg aggaaatcga aacggcgccc cgtcccctgg aagctctcca
    20101 gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca accgcagctg
    20161 cggcgttcag cgcgttatga cgacctggcg cattcagggt gacgcgcatc ggctctttgt
    20221 cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct tctacacgca
    20281 cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc ggtaacaatt
    20341 cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc aggttgtgca
    20401 gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta tccatgtggt
    20461 cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac gatgcatcac
    20521 tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga acccccgccg
    20581 cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg tagatgctgg
    20641 aaaccatcgc ggtggttgtc gttttgccgt gtgttccggc aatggcgatg ccatgacgaa
    20701 aacgcattaa ctcagccagc atttcagcac gacggatcac cggaatacgc gcttcatgag
    20761 ctgcaacaat ttccgggtta tcggcagaaa tcgcgctgga aacaacgacc acgctggcat
    20821 cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc attaactgct
    20881 gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca ttggccagaa
    20941 cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg tgccgaacgc
    21001 gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc attctttacg
    21061 ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg cccgggcagc
    21121 ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag cgcgggcgcg
    21181 ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg cgacagcatc
    21241 cacgctaagc tgtgactgct cgataatttt ggctgcgccc gctttttcca gcggtagcgc
    21301 attccagtat tgctgacggt ctttatgttg aaacggcaca aacaacgccg gtagtcctgc
    21361 cgcggcgatt tcactcaccg ttaacgcacc ggagcggcaa acgacgacat ccgcccacgc
    21421 atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt gtcccgcttc
    21481 ggcatacgcc tgttcaacgg attgttgcga acctttgccg ctctgatgcc agatagtgac
    21541 tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc gtgcgccctg
    21601 ggaaccaccc actaccaaca cacgaaccgg accttcacgt ccagccaaac gttgctgcgg
    21661 caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg cattagggaa
    21721 agcacctgga aacgcctgca tcactttggt agcaatcttc gccagccatt tattggttaa
    21781 gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc acgcggccag
    21841 accacctgga cctgatacat agccgcccat accgagcacc acgtcaggtt tgtacgcttt
    21901 cataatcgcc cgcgcctgac gccaagcgtt gaagatacgc agcggcgcag ctatcagtgc
    21961 ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga tgccatgttt
    22021 tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt gccaaccctg
    22081 agccattaga tggtgcgcaa ccgccagccc cgggaataca tgtccaccgg ttccgcctgc
    22141 catcaccatt aatcgcttcc cttgaccact catcgtgaac ctcgtacaaa cgcctgcgct
    22201 ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt cgacataatc
    22261 agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt cggtaacatc
    22321 cccgccgccg cgcctacgtt aaccagcgcc tggaaactaa accagatacc aatagaacag
    22381 gcgagaaaac cggaaaaacg gtggtcaatt tctaatgctt tgcggccaat cgacatcgcg
    22441 cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata ccccagttct
    22501 tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc cagtttttgt
    22561 accgagttac ctaaaccttg tccccacagt tcaccgcgac caaacgccat caacgattgc
    22621 gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc ggtaacacgg
    22681 cggatacggt acggttcggc gaggatcagc aacacaaccg ctgaaatgcc cataccgata
    22741 atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag cgtagtcaca
    22801 aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc caacaccaga
    22861 attacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc gcctttacgc
    22921 accagatagt tggcgatata gcaaaacagc gacagttttg tcagctccgc aggctgaata
    22981 cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc cactaccaga
    23041 acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg ttgccagaac
    23101 tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag atagacgcca
    23161 tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg cattgacgcc
    23221 gaggtcacca tgataaagcc aatcgccgcg aggccgaagg tcagccacag taaggtgcga
    23281 tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca gccctttagc
    23341 gccgtggaga tccagaccag aatactgaat cctggcaggc gcggcatttt caggcgaggg
    23401 agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg cctcgttgtt
    23461 caaagttctt gaattgatca aggctggcac aggccgggga gagcagaacc atatcgcccg
    23521 gctgaacacg cgtagcaagc aagcgcatcg cctgttccat agtttcggtt tgttctgcca
    23581 cttccgggcg tagcgccgcc agctgcgcgc cgtcccgacc gaaacaatac agacgtacgt
    23641 tatcgccatt caggtaacgc gccagtgggc taaagtccgc cgatttacca tcgcccccca
    23701 gcaacaaatg cagcgtgccg tctacgtgca ggccattcag cgccgcttcc gtactgccga
    23761 cgttggtcgc ttttgaatcg ttaacccagc gcacaccgtt atgctccagc acaacttcaa
    23821 agcgatgcgg cagaccagtg aatgtggtta acgctttcag gctgctggca cgcggtaacc
    23881 ctgcggcatc tgccagcgcc agcgccgcca gcgcattggt gtagttatgc tgcccggaaa
    23941 gtttcatttc tttcacgttc agcactttct cgcctttcac ccgcagccag gtttcaccct
    24001 gctgatgatt caggtgatag tcgcccatgt tgacgccaaa gctgacgcag cgttcatccg
    24061 caccgcgaat cggcattgtt aaggcatcat cagcattaac cacgcaaact tttgcgtttt
    24121 cgtaaatgcg cagttttgct gcacgatact gttgtaaacc aaacggatag cgatccatat
    24181 gatcttcagt cacgttcaga atggtcgcag ctaccgcctg taagctggaa gtggtttcca
    24241 gctggaagct cgacagttcc agcacgtaca gttcgcactc atcatccagc agcatcaacg
    24301 caggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc gccatttcac
    24361 ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt gatcgccaca atcggtgctt
    24421 gtgcttcacg acagaacagc tcgatatcgc caacgatttc gattccggca tcagcggcag
    24481 cgcttaagga tggatgcgcc agtgcaatac cgggactggc gacaatcaga tctgccgcca
    24541 tcagccattc atcattcaga ccgcccgtgt ggcgttctac ggcttcgggt aatttatcca
    24601 ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga gcgaggaaaa
    24661 agtccacgca agaaagcccg gtgaggccca ggccgataat gacgacattt ttaccctggt
    24721 aatcagccat gattaacgta ccttcagcgt tgccagacca atcaggacca gcatcagcga
    24781 aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt catagtggtg
    24841 atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc cgacctgcag
    24901 gatgacggaa agcgtttcta ccacgaacac gccccccata atcaccagca ggaattcctg
    24961 acgcagcagt acggcgataa tacctaacgc accacctaac gccagcgaac ctacatcgcc
    25021 cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagccctg ccccgactat
    25081 cgcggtacag acaatgacca gttccccggc gtgtcgtaga tacggtatgt gtaaatagct
    25141 ggcaaagttc atgttgccgg tcgcccacgc caccagcgca aaaccaccgg cgacaaatac
    25201 ggtcggcata attgccaaac cgtcgagacc atcggtcagg tttaccgcgt tgccagtacc
    25261 cacaatgaca aagtaagcca gcagaatgta gaacagcccc agttgcggca tcacatcttt
    25321 aaagaacgga accaccagct gcgttgcggg cgtgtctttg ccggcaaggt acagcgcgaa
    25381 ggcaacaccc agtgcaatga ccgacatcca gaaatacttc caacgggcaa tcaggccctt
    25441 ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac cgtaacctac
    25501 cagcaccacc agtacgcacc agacgtacgg attggacggg taagcccaca gcagcacgga
    25561 gatcacaatc gccgtcagga tcattatccc gcccatggtc ggtgtaccgc gcttgctgaa
    25621 gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt gcaaatgagc
    25681 aatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc tgacgatggc
    25741 gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca aatgttcggc
    25801 cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc catggcagca
    25861 ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag tgatttaaga
    25921 cgcgcgataa gcgcagtttt atcggaaaaa tgttcgccaa cgccgctggc agtgctgata
    25981 gcatggcttt gtttacccac acttaacacg cggtcaatac cagcagcttt tgccgcttcg
    26041 cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc gcccaccacc
    26101 agcacgcggt agcccggcat ttcagccaga acctgaacag cggcggtcat tgaaccgaca
    26161 ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttttat ggggaacaga
    26221 cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag cgttgcgccc
    26281 acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg ccccgacaac
    26341 ggcagcagaa catcgacgct acctgtaggg gtttgcaggg taaattccgt accgtgtgag
    26401 gttacatgga tattggtggc ggtgaaatcg ctgttggcgg cattaggtga gaaacgccac
    26461 actttgcgtg agccaattac gctctgccag ttcaaccagt cgttgttgtc ggcgttcata
    26521 atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt cgcgacaccc
    26581 gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag cgccgcttcc
    26641 gggcgagtca gactcacagt ccaggcaatt tcaccctgat ggttcgcgcc aagttcaata
    26701 actgcgtaat cgtattccgg cgttaagcgc aacagcgtca tcggtacacc gatgtcgtta
    26761 ttgagattgc ctgccgtata aagtgtgttg ccgcactggc ttaaaattgc cgccgtcatc
    26821 tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc cggaacttgc
    26881 tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac gattaactgc
    26941 ggcagatcaa tatccagcgg acggctaacc agtagtgcgc ctgcgccgcc agctttcgcc
    27001 tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa caggcagccc
    27061 ggcgtcagtt ttcgcgtgtc agtggttaca gcatcaaggg taatatctgc accttgcagt
    27121 tcaccgttga gaatatcggt aagttggcta agggttacgc taatcatgca atcaccccca
    27181 gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca acaatctggt
    27241 aatcctcatg gcctttgccc gcaaccagta ccacatcatt ctctttagcc tgcataacgg
    27301 cgcaagtcac cgcttcagca cggccttcca tcactttggc atatccggca tctaacattc
    27361 ccgccagaat atcgttgatg atggcacgag gctcttcggt acgcgggtta tcgtccgtca
    27421 ccaccgccac gtcagcaaac tcttcagcaa ttgcgcccat cagtggacgt ttgcctttat
    27481 cacgatcgcc accgcagcca aagacgcacc acagcttgcc cgcacagtgc aggcgcgccg
    27541 cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc accgtcggtt
    27601 tgcctggtgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc gcggcggttt
    27661 tcagcaaatc agccagcgga tagccgagtg ccagcagtgt cgccagcgcc aacagcaggt
    27721 tgctgacgtt gaatgcgccc atcaggcgac tttcaatttc cccatcgccc caactcgagc
    27781 taaagcgaat ggtcgcaccg ctgtcgtgat agttcacgtc ggtcgctttc aaccagcgtc
    27841 cgtgacagtt cggattgata tgatcttcca ttgataccgc aaccgcgtcc ggcagttttg
    27901 ccagccagcg gtggcccact tcatcgtcgg cgttaataat cgcctgaccg caatgatgtg
    27961 cagaataaag cagccatttc gcggcttcgt agtgttccat atcaccatga taatcaaggt
    28021 gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc acacggtgtt
    28081 gtaccagccc gtgggatgaa acttccattg cgcaaaaagt cgcgccctga tccaccagcc
    28141 ccgccagctc atgctgaaca tcgactgccg aaccggttgt attttctgtc gggatcactt
    28201 tccccagcag gccgttacca acggtgccca ttaccgcgct ggtttcgcca agcagttggc
    28261 tccactgcgc caacagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt acgcccacga
    28321 gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat aaacgctcgt
    28381 tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca ccgtcggtcg
    28441 cctcatcttt cgcctctgca ataatggcag ccacaccttg cgctatcgcc tgcgggatat
    28501 atcgacgccc gtccgcctga tgacctacta cagctacaaa gagatcgccc gccgcagcca
    28561 cacggctgtc gagtgtcatc tctcgcagtg ctcgcgaagg tgcgtctggc acccacggag
    28621 caagaaggtc gcgcaaatta cgatctgcca cctgtaccct cgccttgatt aatcacaaat
    28681 tcatttttat cgcccgttgt cagcgcatcc ggctcgatgt tcatggtacg caatacgccg
    28741 cccatgatgg caccaaagac cggcgcggaa acggcgccgc cgtagtattt acccgcctgc
    28801 ggatcgttga taacaacaac cagcgcgaag cgcggctgac tcgcaggcgc aacgcctgcg
    28861 gtataagcaa tatatttatt gatgtagcga ccgtccggcc cgaccttttt cgcggtaccg
    28921 gttttaatgg cgatacgata gcctttaatc gccgccttca cgccgccgcc gcccggtagc
    28981 gccacgcttt ccatcatatg caccacggtg cgaacaatgg attccgggaa gacacgttca
    29041 ccgggaaccg gggggtcaac tttggtaatc gacagtgggc gataaatgcc gtagctgccg
    29101 atagttgcgt agactcgcgc taactgtaac ggtgttacca ttagcccgta gccgaaagag
    29161 aaggtggccc tctctatgtc agaccaccgt tgtttttgag gatataagcc actgcgttct
    29221 ccgaccaacc ccaaattggt cgcttttccc agtccaaaac gtgagtaagt atctactaac
    29281 gctgaggacg gcatcgctaa cgccagtttg gaaacaccga cgttactcga cttctgtaat
    29341 accccggtca gggttaattc gctgtagcgc gccacgtctt tgatttcgtg gccgttaatt
    29401 cgataaggaa cggtattgag taccgagttt tcccgcacca cgccacgttg caacgcggtc
    29461 atcaccacca tcggtttaac cgttgagccc ggttcaaaca cgtcggtgat ggtacggtta
    29521 cgcatcgctt ctttcggcgt gccgctcaga ttatttgggt tgtatgacgg gctgttagcc
    29581 atcgccagca cttcaccggt gttgacatcc accagcacgg cgctaccaga ttcagccttg
    29641 ttaaaggcca ctgcgttgtt cagttcgcga taaaccagcg cctgcaggcg ttcatcaata
    29701 ctcagcgtca ggttgtgcgc tgcctggctg tcagtagaag aaatatcttc aattacgcga
    29761 ccatagcggt ctttacgcac aatgcgctca cccggctgcc cggtaagcca tttatcgaaa
    29821 ctcttctcga cgccctcaat cccttggcta tcgacgttag taaaaccgat gaggtgagca
    29881 gtcacttcgc cggatggata ataacgacga gattcttcac gcagatgaat ccccggcagt
    29941 ttcagttttt tgatgtagtc cgccatgtca gggtttacct gacgcgccag ataaataaag
    30001 cgccctttcg ggttggcgtt aatgcgggct gagagctgat ccagcggaat attgagcgcg
    30061 ttagccagcg ccttccagcg gtcaccgacg ctgataccgc cagcgtcatg cacttctttt
    30121 gggtcagccc aaatcgcttt tactggcaca ctcaccgcta acgggcgacc agaacggtca
    30181 gtaatcatgc cgcgggaggt ggaaacttgc tgaacgcgaa gagaacgcat gtcgccctct
    30241 ttcaccagca tatccgggga gataacttgt aaccacgcta cgcgtccgag cagaaaagcc
    30301 agcgccagga gaatacagcc gcataacaac gcaaaacgcc aactgataaa gttggcatgt
    30361 tcttcctgac gttttggttt ctgcgttttc gccgctgctt tcatgcgtcg cgtttatcct
    30421 tatttttgca ctacgatatt ttcttgtgac ggatcaacat gctgcatttg cagcttttcc
    30481 gtggctattc tttccacccg gctatggtcg ccgagcgcat tctcttcaag gatcaggttg
    30541 cgccattcaa tgtctaaagc atctcgctcc agcaccagtt gttcgcgctg agcggtcagt
    30601 aaacgggtat ggtgcgccgt ggttaccacg gtcaccgccg tcaaaataat gcaaatgaac
    30661 aggcagagtg gcagcttccc aaatcgcaaa agatcgtcac cgataacgcc aggcaaggca
    30721 tggcgctcgt ggcttcccat cgatccttta actttgctta gagcttctgt cactctgctg
    30781 atcatgcgtt cgtcctctct gcaatacgca gaactgaact acgggcacga gggttctcag
    30841 ccacctcttc ttcgcccggc atcaacttac ctagtgctcg caactgacgg ccccccagtt
    30901 ttttgagctg ctcttctgtc atcggtaacc ctgccggaac ttgcggaccg cggctgtttt
    30961 cacgcataaa acgtttcaca atgcggtctt caagtgagtg gaagctgatg atcgaaagcc
    31021 gcccacccgg ggccagcaca ttgagcgagc tttttagcgc ctgctctatc tcctccagtt
    31081 cactgtttac ccaaatgcgc accgcctgga aggtacgggt cgcgggatgt ttaaacttat
    31141 ctttcaccgg cgttgcggca gccacgactt ccgccagttc tttggtgcgg gtcatcggct
    31201 gttcgcggtt acgctcgaca atggcgcggg caatgcgttt ggcaaaacgc tcttcaccat
    31261 aggttttcaa tacccaggcg atatcggctt cttctgcggt ttgtagccat tcagcggctg
    31321 actgcccacg ggttgggtcc atacgcatgt ccagcggacc atcgcgcata aaggaaaagc
    31381 cgcgctcagc atcatcaagt tgcggtgaag agacgccaag atcgaggaga atgccgtcga
    31441 tcttgccgat aagatcgcgc tcggcaacat attcgcccag cgcggagaaa ggtccgtgga
    31501 tgatggagaa gcgcggatca tcaatagtct tcgcaacggc gatagcctgc gggtcgcgat
    31561 cgatcgccag caaacgcccc tcttcgccaa gctgcgagag gatcagacgt gagtgaccac
    31621 cgcgaccaaa agtcccatca atgtagatgc catcaggacg gatattgagg ccattaacgg
    31681 cttcatccag cagcaccgta gtatgtttat agttttccat cattttatag agacaagtcc
    31741 tgcagtcgct ccgataagtc tccggtagcc aactgctctg cgtcgatatc ttccttgacc
    31801 tgttgatgcc aggttgtttc atcccacagc tcaaacttgt tgaactgtcc aaccagcatc
    31861 acttcttttg tcagcccggc atgttgccgc agtactggcg cgattaacaa tcgacctgcg
    31921 ccatccatct gacattcgct ggcatgacct aacagtaggc gctgcacacg gcgctcaacc
    31981 gggttcatgc tcgacagacg cgataatttt tgctcgataa tttcccattc aggcaggggg
    32041 taaagcagca ggcacgggtg atgaatgtca atggtgcaaa ccatttgacc ggcagcattc
    32101 tcaagcagct gttcccgata acgggtaggc actgataagc gccctttgct gtcgagattg
    32161 actaacgttg ctccccggaa catgccagcc tcacccctta ttcccacttt gccccacaat
    32221 ttcccactga aaggagttta cggagcggag gaaaagcttg tcaagccagt cacaaggcat
    32281 accgaggtaa aaaacccgat gtttacggct aatatcagcg ttgtttattt tacgtccaac
    32341 gtgcgaagca aattaacgtt atgaatagct gtaagaaaaa atccctcatc gtcatcaccg
    32401 ttttaaaatg actcactatt ttccacagca aatataaagt gtcagtttgc gacgcgagcg
    32461 gcattttagg acatatcttc cccggttaac agtccctgtt gcgtctgtgt ggcgcttgat
    32521 gccagcgggt ttgcgcaaaa gcaaagaaaa gtgtttgtta aatcggcaat tcatctctgc
    32581 atgtaacaaa ataatgcaaa aacgcgctat cgatatcact taattattgt gaatttcagc
    32641 tggtgaggta attaacgggc aggtttaaaa gcatttatca ataatagcca ggaatagtct
    32701 tatttacttt aaggatattg atgtccattc ccgtactcaa cgcgccagag agaaattcac
    32761 ctggcgcgta tttttgttcg cggcttagct acggctgagc acgccgcggc gatagagatt
    32821 acgtttaatg cgcgttaaac caggttttgg cttacgcggt tcgtccaggc ttgccaggac
    32881 aatctccagc acacgctctg cgacatcgcg gtgacgttga gccactgcca gcaccgggca
    32941 ctgtaagaag tcgagcagtt cgttatcgcc aaaggtggca attgccaggt cagaaggcag
    33001 tttgccgtca cgacgtagcg tgacatccat caccccttgc aacaacgcaa acgacgttgt
    33061 gaacagtgcc tgcggcatcg gatgcgtttc cagccatttt tcgaataact gggcagccgc
    33121 ctcccgctca tagctgttgg cataaaggaa atgcacttcg cgcggatcat ctttccaggc
    33181 agtacggaaa ccttgttcac gcaggaagct gacagaaagc tccggcagcg cgccaagata
    33241 aagtaccgtc tcggcgggaa acttacgtaa ctcttccgcc agcatttcgg catcatcctg
    33301 atcggcacca accacgctgg tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat
    33361 cgggaacggg tcgttagccc agcgttgata aaaaggatgc tcaggtggca acgacgtcga
    33421 aacaataatg gcatcaacct gacgctgtaa aagatgctcg atacaacgca tttcgttgtc
    33481 gggctgatct tccgagcagg caatcagcag ttgataaccc ctttgccgcg cctggcgttc
    33541 aagatagtta gcgatgcggg tatagctggt gttctccaga tcggggatca caagaccaat
    33601 agaacgtgtg cgtccagcac gaagcccagc tgccacggcg ttcgggtggt aattgtgctc
    33661 acgcaccaca gccatgactt tttcaacggt tttgtcgctc acacggtatt gcttcgcttt
    33721 gccgttaata acatagcttg cagtggtccg cgacactccc gccagccgag cgatttcatc
    33781 cagtttcaca attgcccctt gcgtaaaatg taaaaaccat agccattgta ctggtatgga
    33841 ttaaattcat taacatctaa gcgcagaaaa ttaactgcgg caactgcttt tattccaggt
    33901 tcccactgat ttcgcaaaaa aagcccaacg tgacatgttg ggctgcaaat tgcgcattga
    33961 gatcattctc aacgcattat tttatcgccg cgcgaaagtc cgaccacacc agagcgagca
    34021 acttccacaa ttttcgccac atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg
    34081 gtgcctgcta attgaacggt ataaagcgag ggtgtgacat cgataatttg cccacggaat
    34141 atttccgtat tacgtttcac ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc
    34201 atgatttccc gctcaacgtg cgcgccctgc cccaactcgc tcacgcgcaa gacatcgacc
    34261 agcttgtgta attgcttttc gatttgctca agcacttttt catcgcccac ggtctggatg
    34321 gtcatacgcg ataatgtcgg atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag
    34381 ccacgctggg aaaaaaggcc aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag
    34441 actgataata tccggcgcat aatcaggttc tctccgtttt gcttaaccac atttcatcca
    34501 ttccgccccc gcgaatctgc atcgggtaga cgtgctcact gccatcgacg gtaacatcaa
    34561 caaacaccag gcgattattg cgcacctgtt ccagcgcctc gctaagtttg ctttccagtt
    34621 cttgcggatg agagatctgg atcccgacgt gaccataggc ttccgccaga cggacgaagt
    34681 ctggtaagga ctgcatataa gattgtgaat gacggccgga atagatcatg tcctgccact
    34741 gcttcaccat ccccagatag cggttattga gattcaccac cagtacgggc aactcatatt
    34801 gcaacgcggt agacagttcc tggatgttca tctgaatact gccgtcacca gtgacgcaaa
    34861 ccacggtttc ttctggcaac gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca
    34921 tcgtgccgag gccgccagag ttgatccagc gacgcggttt gtcgaatgga taataaagcg
    34981 cagcaaacat ctggtgctgc ccgacatcgg acgtcacgta agcgtcccca tgagtcaacc
    35041 gccaaagagt ctcgatcacc gcctgcggtt taatcttttc actgtgagtg tcatatttca
    35101 ggcactgacg agcgcgccac tgttcaatct gctgccacca gtcgcggatc tcatccagcg
    35161 gttgatgggc ggattcttgc gacaggagtt caagcatttg ttctaggacc tggcgagcat
    35221 cccccacaat cgggatatca gcagtcacgg ttttagaaat tgaggttgga tcaatatcga
    35281 tatgcagaac agtggcattt gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa
    35341 aacgtacccc gacggcgaaa atcacatccg cgttatgcat cgtcatattg gcttcgtagg
    35401 taccgtgcat tcccaacatg cccagtgcct ggcgatgcgt tgccggaaac gcccctagcc
    35461 ccatcaaaga ggaaacaacg ggcagattca acgcctccac cgcttctttc aactgcagat
    35521 ggcagcccgc cgtgattgcc ccaccgccta cgtagacaac cggttttttt gccgccacca
    35581 gcgtttgcag agcgcgctta atttgccctt tatgcccgga ggtcgtggga ttgtaagaac
    35641 gcatactgac cgactccggc cagacatagg gtaatttgtt cgccggattt agaatatctt
    35701 tcggtaaatc aacgaccacg ggtccaggac gaccacttgc cgctagccag aaagcctttt
    35761 tcagcacctg cggaatgtct tccgtttgct taaccagaaa actgtgttta accaccgggc
    35821 gagaaatccc caccatgtcg cactcctgaa aggcatcata acctatcaac gaagtcgcta
    35881 cctgcccgga aaggacaact aatggaatgg aatccatata agcggtggca atgccagtaa
    35941 ttgcattggt cgcccctgga cccgacgtta ccagcacgac gccgacttcc ccggtcgcgc
    36001 gcgccagacc atcggccata tgcaccgccg cctgctcatg gcgaactaaa acatgatcga
    36061 taccacccac ggtatgcaat gcatcgtaaa tatcaaggac cgcgcctcca ggataaccga
    36121 atacttgttt aacgccctga tcgataagcg atcggacgac catctcggct ccagacaaca
    36181 tctccatggc ctgcctcact gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga
    36241 tcttgcttaa gcaattggca gacagagaca taaacacggg gtaataagaa taaacaggag
    36301 gaaaggtgaa aagaaacgat aaaatcctcc attacagagg atgaaattga taataaatcg
    36361 gctgaatcct acaacttaca gcaatttctc tatttttt
//
LOCUS       DADSZD010000027        36318 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.027, whole genome shotgun sequence.
ACCESSION   DADSZD010000027 DADSZD010000000
VERSION     DADSZD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.027"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            110..352
                     /locus_tag="J1Z28_001234"
     CDS             110..352
                     /locus_tag="J1Z28_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716081.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTR"
     gene            467..1105
                     /locus_tag="J1Z28_001235"
     CDS             467..1105
                     /locus_tag="J1Z28_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBA7716082.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIIDGATWTRTMGWTEYQQVRYATARSVFKWNGTDTVKVGSDET
                     PVRVLDEEVFTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1102..1839
                     /locus_tag="J1Z28_001236"
     CDS             1102..1839
                     /locus_tag="J1Z28_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBA7716083.1"
                     /translation="MIKQTIVALLLSAGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1839..3935
                     /locus_tag="J1Z28_001237"
     CDS             1839..3935
                     /locus_tag="J1Z28_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBA7716084.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLVQKRVEPVVPKFTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3982..4260)
                     /locus_tag="J1Z28_001238"
     CDS             complement(3982..4260)
                     /locus_tag="J1Z28_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716085.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4475..4885
                     /gene="psiE"
                     /locus_tag="J1Z28_001239"
     CDS             4475..4885
                     /gene="psiE"
                     /locus_tag="J1Z28_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBA7716086.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4979..5869)
                     /gene="malG"
                     /locus_tag="J1Z28_001240"
     CDS             complement(4979..5869)
                     /gene="malG"
                     /locus_tag="J1Z28_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBA7716087.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(5884..7428)
                     /gene="malF"
                     /locus_tag="J1Z28_001241"
     CDS             complement(5884..7428)
                     /gene="malF"
                     /locus_tag="J1Z28_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBA7716088.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(7582..8772)
                     /gene="malE"
                     /locus_tag="J1Z28_001242"
     CDS             complement(7582..8772)
                     /gene="malE"
                     /locus_tag="J1Z28_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBA7716089.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            9137..10252
                     /gene="malK"
                     /locus_tag="J1Z28_001243"
     CDS             9137..10252
                     /gene="malK"
                     /locus_tag="J1Z28_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBA7716090.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10324..11664
                     /gene="lamB"
                     /locus_tag="J1Z28_001244"
     CDS             10324..11664
                     /gene="lamB"
                     /locus_tag="J1Z28_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBA7716091.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            11896..12816
                     /gene="malM"
                     /locus_tag="J1Z28_001245"
     CDS             11896..12816
                     /gene="malM"
                     /locus_tag="J1Z28_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBA7716092.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFSSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            12995..13492
                     /gene="ubiC"
                     /locus_tag="J1Z28_001246"
     CDS             12995..13492
                     /gene="ubiC"
                     /locus_tag="J1Z28_001246"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBA7716093.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            13505..14377
                     /gene="ubiA"
                     /locus_tag="J1Z28_001247"
     CDS             13505..14377
                     /gene="ubiA"
                     /locus_tag="J1Z28_001247"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBA7716094.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(14532..16955)
                     /gene="plsB"
                     /locus_tag="J1Z28_001248"
     CDS             complement(14532..16955)
                     /gene="plsB"
                     /locus_tag="J1Z28_001248"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBA7716095.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            17126..17494
                     /locus_tag="J1Z28_001249"
     CDS             17126..17494
                     /locus_tag="J1Z28_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBA7716096.1"
                     /translation="MANNTTGFIRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            17604..18212
                     /gene="lexA"
                     /locus_tag="J1Z28_001250"
     CDS             17604..18212
                     /gene="lexA"
                     /locus_tag="J1Z28_001250"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBA7716097.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            18286..19611
                     /gene="dinF"
                     /locus_tag="J1Z28_001251"
     CDS             18286..19611
                     /gene="dinF"
                     /locus_tag="J1Z28_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBA7716098.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            19727..19936
                     /locus_tag="J1Z28_001252"
     CDS             19727..19936
                     /locus_tag="J1Z28_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBA7716099.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(19978..20493)
                     /gene="zur"
                     /locus_tag="J1Z28_001253"
     CDS             complement(19978..20493)
                     /gene="zur"
                     /locus_tag="J1Z28_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBA7716100.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            20649..21644
                     /gene="dusA"
                     /locus_tag="J1Z28_001254"
     CDS             20649..21644
                     /gene="dusA"
                     /locus_tag="J1Z28_001254"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBA7716101.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            21778..22020
                     /gene="pspG"
                     /locus_tag="J1Z28_001255"
     CDS             21778..22020
                     /gene="pspG"
                     /locus_tag="J1Z28_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBA7716102.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(22185..23168)
                     /locus_tag="J1Z28_001256"
     CDS             complement(22185..23168)
                     /locus_tag="J1Z28_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBA7716103.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            23251..24666
                     /locus_tag="J1Z28_001257"
     CDS             23251..24666
                     /locus_tag="J1Z28_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase DnaB"
                     /protein_id="HBA7716104.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            24719..25798
                     /gene="alr"
                     /locus_tag="J1Z28_001258"
     CDS             24719..25798
                     /gene="alr"
                     /locus_tag="J1Z28_001258"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBA7716105.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLNEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            25821..26378
                     /locus_tag="J1Z28_001259"
     CDS             25821..26378
                     /locus_tag="J1Z28_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HBA7716106.1"
                     /translation="MSYSEIAACLAYAVSVWLAARNNVHTWWIGIIGSILYGWVFWSV
                     QLYADVTLQLFFIATSITGWIHWLKGQGGDILPVRRTQASHFFLLLLCAVVVAGGYGF
                     LLHTFTNAWAPWLDSLILTFSVLAQFMLMGRRIENWFVWLAVNTLAVPLYMTRGLNLT
                     AGLYFLFWINAWHGLYQWRKELQTS"
     gene            26375..27379
                     /locus_tag="J1Z28_001260"
     CDS             26375..27379
                     /locus_tag="J1Z28_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA7716107.1"
                     /translation="MKPFATGLVVGKFAPLHCGHEKLINTALAQCEELFIISYSVPEM
                     PDCEPEKRLTWLQVRFPQATILVLTPELVARYNLPAIPHNDADADIHRHYVATLCLQI
                     LRCRPHAVFTAEDYGDGFANVLARRFAQPVEHVRMARPVGDEAPSGTLIRSDVHRYRY
                     MLANDVYYSFVRRICLLGGESTGKSTLSKALADGLDTVYVAEFGRDYWEEKNGILTAD
                     DLLHIACEQVRREQQAEANHYLICDTSPLTTLFYALDQYGHAPQELHQLAEREYSVVV
                     LCGAEFPFVQDGTRQGEVFRARQQVWYEQELSRRNIPYLSVSGPLQERVEQILRQLPD
                     "
     gene            27461..28654
                     /gene="tyrB"
                     /locus_tag="J1Z28_001261"
     CDS             27461..28654
                     /gene="tyrB"
                     /locus_tag="J1Z28_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBA7716108.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYNEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PEHNFDYLLNQRGMFSYTGLRTAQVDRLREEFGVYLIASGRMCVAGLNAQNLHRVAKA
                     FAAVM"
     gene            28836..31580
                     /locus_tag="J1Z28_001262"
     CDS             28836..31580
                     /locus_tag="J1Z28_001262"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBA7716109.1"
                     /translation="MENMTSPGTLLSGDNATWLEDYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAIIQLINAWRTQGHLRAKLDPLGLNPPADVPSLLPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNAPQADVQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPIDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDGAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            31613..32767
                     /gene="odhB"
                     /locus_tag="J1Z28_001263"
     CDS             31613..32767
                     /gene="odhB"
                     /locus_tag="J1Z28_001263"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBA7716110.1"
                     /translation="MIEITVPVLPESVTEGTLTTWCKQEGEHVKRDDVIAELETDKVI
                     LEIPAPHDGVLSNIIVSEGSTVTSAQLLAHLKPQAVIEETVTPVTETLAMPSARLEAQ
                     RSGVELADVAGSGRNGRILKEDVQRVTPAPVIQPERVAEIAPAKPLTPGARQERREPM
                     SRLRQRIAERLLASQQNNAILTTFNEVNMQSVMDLRARWKDRFAEKHGVKLGFMSFFV
                     KAVTRALERFPVVNASVDGNEIIWRDYCDIGIAVSSNRGLVVPVLRNAQSLSLVEIER
                     QIAEYATQARNGKLPLEALQGGTFSITNGGTFGSMMSTPIINPPQSAILGMHAITPRP
                     VAENGQVVIRPMMYLALSYDHRIIDGQEAVQTLVAIRELLESPEQLLLDL"
     gene            32779..34197
                     /gene="lpdA"
                     /locus_tag="J1Z28_001264"
     CDS             32779..34197
                     /gene="lpdA"
                     /locus_tag="J1Z28_001264"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA7716111.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLHVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            34219..35388
                     /gene="sucC"
                     /locus_tag="J1Z28_001265"
     CDS             34219..35388
                     /gene="sucC"
                     /locus_tag="J1Z28_001265"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBA7716112.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            35401..36273
                     /gene="sucD"
                     /locus_tag="J1Z28_001266"
     CDS             35401..36273
                     /gene="sucD"
                     /locus_tag="J1Z28_001266"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBA7716113.1"
                     /translation="MSVLINKHTRVLVQGITGKNGTFHTEQAIAYGTQIVGGVTPGKG
                     GQRHLDRPVFDSMKEAMRQTEADASVIYVPAPFVLDSVVEAIEAGVKLVVVITEGVPT
                     LDMVKVRRLLDCHPDVRLIGPNCPGVITPGECKIGIMPGEIHRPGRIGIVSRSGTLTY
                     EAVAQTTALGLGQSTCIGIGGDPVPGTNFIDALRLFENDPQTDAVIMIGEIGGNAEER
                     AAEFIRHEMNKPVVGYIAGVTAPKGKRMGHAGAIISGGSGSAEDKFRAFDKAGMAWTR
                     NPALLGETLWSVIK"
BASE COUNT         8497 a         9105 c         9942 g         8774 t
ORIGIN      
        1 cccatcacta attccccgcg ccgtaattcg catgctttag ttgtgtatac tcgatcccgc
       61 ccgaaatgtt ttgggtaaat ctccattcat tcaatgaagg gaaattgtta tgaaaaaagt
      121 tctgtatggc atttttgcca tatctgcgct tgcggcgact tctgcgtggg ctgcacctgt
      181 acaggtgggc gaagcggcag gatcggcagc aacgtcggtt tcggcgggga gttcctccgc
      241 gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg ctcgcggcaa ccggtggcgg
      301 tgatggttct aataccggga ccaccacaac cacgaccacc agtacccggt aataaagtat
      361 gtatccccaa aataattcga gtcatcgcat ctgaagcttg aagtatgaag gggattaacc
      421 ataaccacac tccggtgtgg ttattctgcc cctctggaga agagtcgtga agcgacctgc
      481 actcattctt atctgcctgc tattacaggc ctgttcagcc acgactaaag agctgggcaa
      541 ttcactgtgg gacagtctgt tcggtacgcc aggcgtacag ctgacggacg atgatattca
      601 aaatatgccc tacgccagcc agtacatgca gcttaatggc gggccgcagt tatttgtggt
      661 gctggccttc gctgaagacg gacaacaaaa atgggtcact caggatcagg ccacccttgt
      721 cacacaacat ggtcgtctgg tgaagacttt gcttggtggc gacaacctga ttgaagtgaa
      781 taaccttgcc gccgacccgc tgattaaacc cgcacaaatc attgatggtg caacctggac
      841 gcgcacgatg ggctggaccg agtaccagca ggtacgctac gccaccgcgc gctcagtctt
      901 caaatggaat ggcaccgata ccgtcaaagt cggcagcgat gaaaccccgg ttcgcgtgct
      961 ggacgaagaa gtcttcactg accaggcgcg ctggcataac cgctattgga tcgacagcga
     1021 agggcaaatt cgccagtcgg aacagtatct cggcgcggat tacttcccgg tgaaaaccac
     1081 cctcatcaag gcggcaaaac aatgattaaa caaactattg tcgcgttgct tttgagtgcg
     1141 ggggcgtcat cagtcttcgc ggcgggaacc gtcaaggtgt tcagcaatgg cagcagtgag
     1201 gccaaaacgc tgacgggcgc agagcattta atcgatctgg taggccaacc gcggctggca
     1261 aacagctggt ggcccggagc ggtgattagc gaagagctgg ccacggcggc tgcattgcgt
     1321 cagcagcagg cgttgctgac acggctggca gaactggctg cagattccag cgccgacgat
     1381 gccgccgcga ttaacgcctt acgccagcaa attcaggcgt tgaaggtgac gggcaggcaa
     1441 aaaatcaatc ttgatcctga tatcgtccgc gttgccgaac gcggtaaccc gccgttgcag
     1501 ggcaactaca cgctgtgggt cggaacgccg ccgtccacgg tcacgttgtt cgggcttatc
     1561 agtcgtcctg gcaagcagtc attcactcca ggtcgcgacg tggcgagcta tctctctgac
     1621 cagagcctgc tcagcggtgc ggatcgcagc tacgcgtggg tggtttaccc ggacggacgc
     1681 acgcaaaaag cgccggtggc ttattggaac aagcgtcacg tagagccgat gcccggcagc
     1741 attatttatg ttggcctcgc ggactccgtc tggagtgaga cacctgatgc ccttaacgcc
     1801 gacattcttc agactctgac gcagcggata ccccaataat gaaaaaaaga catctgctta
     1861 gcttactggc gctgggcatt agcacagctt gctacggcga aacatatcct gcgcccattg
     1921 gtccgtcgca atcggatttc ggtggcgtag gattattaca aacgcccacc gcgcgcatgg
     1981 cgcgggaagg ggagctgagc ctgaactatc gcgataacga tcagtaccgt tattactcag
     2041 cttcagtgca actcttcccg tggctggaaa caacgctgcg ctacaccgac gtgcgcaccc
     2101 ggcagtacag cagcgtcgaa gcgttctctg gcgatcaaac gtataaagat aaagccttcg
     2161 atctcaaact gcgtttgtgg gaagagagtt attggctgcc gcaagtggcg gttggtgcgc
     2221 gggatatcgg cggtacgggg ctgtttgatg cggaatatct tgttgccagc aaagcctggg
     2281 ggccgttcga ttttacgctc ggcctgggat ggggatatct gggcaccagc ggtaatgtga
     2341 aaaatccgct ctgttcagcc agtgataaat attgctatcg cgataacagc tacaaacaag
     2401 cgggatctat cgacggcagt cagatgttcc acggcccggc ctcactgttt ggcggcgtgg
     2461 aataccagac gccctggcaa ccgctgcgcc tgaaactgga gtatgaaggt aataattatc
     2521 agcaggattt tgccgggaag ctggagcaaa aaagtaagtt taacgtcggg gcgatttatc
     2581 gcgttaccga ttgggccgac gttaacctta gctatgaacg tggcaacacc tttatgtttg
     2641 gcgtcacgct gcgcaccaac tttaacgatc tgcgcccgtc ctacaacgat aacgcccgac
     2701 cgaaatacca gccgcaaccg caggatgcca ttttgcagca ctcggtggtg gcgaatcagt
     2761 taacgctgtt gaaatacaat gccggactgg ccgatccgca gatccaggcg aaaggcgata
     2821 cgctgtatgt caccggcgag caggtgaaat atcgtgattc gcgcgaaggg atcatccgtg
     2881 ctaatcggat cgtgatgaac gatctgcctg atgggatcaa aacgatccgc attacggaaa
     2941 atcgccttaa catgccgcag gtgacgacgg aaaccgatgt cgccagcctg aaaaatcatc
     3001 tcgccggaga gccgttgggc cacgaaacga agctggtgca aaaacgcgtc gagccagtgg
     3061 ttccgaagtt caccgagcag ggctggtata tcgacaaatc acgctttgat ttccatatcg
     3121 atccggtgct gaaccagtcg gtcggtggcc cggaaaactt ttacatgtat cagctgggcg
     3181 tgatgggaac ggcggatttg tggctgacgg accatctgct gaccaccggc agcctgtttg
     3241 ctaaccttgc caacaactac gacaagttta actacaccaa tccaccgcag gactcgcact
     3301 taccgcgcgt gcgtacccat gtgcgcgagt atgtgcagaa tgatgtctat gtgaataacc
     3361 tgcaagccaa ctacttccag catctgggca acggcttcta cggtcaggtc tacggaggtt
     3421 atctcgaaac catgtttggc ggcgtggggg cagaagtgtt gtatcgcccg ctggatagca
     3481 actgggcgtt tggtctggat gccaactacg ttaaacagcg cgactggcgt agcgcaaaag
     3541 atatgatgaa attcaccgac tacagcgtga aaaccggaca tctgaccgcg tactggacgc
     3601 catctttcgc ccaggacgtg ttagtgaaag cgagcgtcgg gcagtatctg gctggggata
     3661 aaggcggcac gctggagatc gccaaacgct ttgatagcgg cgtggtagtg gggggctatg
     3721 ccacgatcac gaatgtttcg aaagaggagt acggcgaagg ggacttcacc aaaggcgtgt
     3781 atgtctctgt accgctggat ctcttctcgt ctggcccgac acgcagccgt gcggcgattg
     3841 gctggacgcc gctgacgcgt gacggtggtc agcaacttgg gcgtaagttc cagttgtatg
     3901 atatgaccag cgaccgtagc gtcaatttcc gctaagtcat gggaaaggtg ccagttttcg
     3961 cactcactgg caccttcatt cttaatggcc tgtcgagcca tcaggaacgc agttcacccc
     4021 cggtttgcga tctggtgtgt aaaggcgaaa acattctgtc ttcctgtccg tcgatatcgg
     4081 ttgcaggtct ggttgcctga gcgactgggc catcattgga ctggagacat tgctttgcgg
     4141 taggtcggct ttatcagcag taccgagcgg accagcataa gcaggaagaa cagagactga
     4201 taacatcaaa gcagcaaaat aaggcttcat ttttaccacc tttatcaggt tacgtttcat
     4261 ttgttccaga ggaacattgt cgatttttcg cgcattgctg gtggctggga atcacctgaa
     4321 tgggtgattt ttttgggctg tgtgttttgc cagatgcgac gttgatggga gatgcccact
     4381 taattgagct gaacaaaaca tacacaaaaa atatagatct ccgtcacatt tttgcgttat
     4441 acaggaagct cgccactgtg aaggaggtac tgctatgacg tcactctctc gtccgcgcgt
     4501 ggagtttatc tccaccattt tgcagaccgt actcaatctt ggcctgttgt gcctcggcct
     4561 gattttggtt gtcttcctcg gcaaagaaac ggtgcatctg gctgatgtgc tgttcgcgcc
     4621 agaacaaacc agtaaatatg agctggtaga agggctggtg gtttactttc tctatttcga
     4681 atttatcgcg ctgattgtga agtactttca gtccggtttt cacttcccgc tacgttactt
     4741 tgtctatatt gggatcaccg ccattgtgcg cttgatcatc gtcgatcata aatcgccact
     4801 ggatgtgttg atctactcgg cagcgatcct gttactggtg atcaccctgt ggctgtgtaa
     4861 ttcgaaacgg ctgaagcggg agtaaaaaaa ggcgctccgt ggagcgccga ataacagtca
     4921 caagttggga taacgtaagt tgagggtgca gcggcataac attggcagaa caacatcttt
     4981 aacctttcac accacctgcc gtcaggccgt tgaccagcca gcgttgagcc agcaagaaga
     5041 cgatggtgat cggtaatgca gacatcacgg cagcggcggc aaagtcaccc cacaggtagt
     5101 tttgcgggtt gaggtattgc tgcatcccca cggccagggt gtagctgttt acgtcacgca
     5161 gtaatagcga cgcgaccgga acttcagtaa tggcagcgat aaacgacagg atgaacacaa
     5221 ccgccagaat cggtactgac agcggcaaca ggacaaggcg gaaggcctgc cacggtgtcg
     5281 caccatccag cgccgccgct tcttccagcg aactgtcgat ggtttcgaaa taacctttga
     5341 tggtccagac atgcagcgca atcccaccca gatacgcgaa aattacgccg ccgtgagtat
     5401 tcaggccaat gaatggaatg tactcaccca gacgatcaaa caacgcatac aacgcgacca
     5461 gtgaaagtac tgccgggaac atctggaaaa tcagcattcc tttcagcagc gtcgctttgc
     5521 ctggaaagcg catacgggcg aaagcgtagg cgcaggtggt ggagagcgcc acaatgccaa
     5581 tcgcggaaat cccggcgacc tttaccgagt tccacagcca cagcagtacc gggaatggcg
     5641 gtggcgtaat gcgaccatca gcctgttcca cgctaaaacc taacgccagt ttccagtgat
     5701 cccaggagat ttgctccggg atcagactac cggtcgcgaa gttcccctga cgcagcgaga
     5761 tggcgacgac catcagcagc gggaacataa ttgccgcgat aaaaagtagc aatagcaggt
     5821 gagtaataaa caaacgtgct ttttgcgatt tcggttggac cattgccatt tttgttatct
     5881 cccttaatca aacttcattc gcgtggcttt caggttcact atcgccagcg cgcccaccag
     5941 caggaagatc agcgtggcaa ttgctgctgc cagaccgaag tcctgacccc cgccgccttc
     6001 aaaagcgata cggtaggtgt agttaacaag caggtcggta taaccggctg gcgtggtcgt
     6061 gccaagacga tccgggccgc cgttggttaa cagttgaatc agcacgaagt tgttaaagtt
     6121 aaaggcaaag ctggcgatca tcagcggtgt cagcggttta atcagcagcg gcagcgtaat
     6181 cttaaagaag ttctggaatg gacctgcacc atccattgct gaggcttcat acaaatcgtc
     6241 cggaatcgct ttcagcaagc ccatgcagag gatcatcatg tacggataac ccagccaggt
     6301 gttgacgata atcagcatcg tgcgggcggt ggtcggatcg ctgaaccagg cgggcttcac
     6361 gccaaacagc gcgctcaaca tcatgttgat ttcaccgaaa ctctggttaa acaacccttt
     6421 gaaaatcaag attgaaatga acgatggcac cgcgtagggc agaatcagca ggacgcgata
     6481 gaccgctttg ccgcgcaacg cttcccactg caccagacac gccagaacca tgccgaccgc
     6541 caccgttaaa aagacagtga tcagcgagaa caccacggtc cagacgaaaa tggcgaggaa
     6601 cggtttctga atgccttcgt cggtaaagac gcgggtaaag tttttccagc cggtggtcac
     6661 ggtgtaaccg gggcttagct tttcatcccc ccagttgcca tcggcggtaa tggactggta
     6721 aaagccaatt tggttattcg gacgatattt cacgccgctc tggttattcg tcaacgtgcc
     6781 gtcaccgtcg agtgtgtaga gcggttgcgt gccagaaaac tggcgcaggg agctcatcat
     6841 cactttgttg ccatccggca gaatggcggt aatgtcactc agtgcctgac gattctgggt
     6901 aatcacgcgc agattcgcgc gctcgccttc aggctgcgtg gcgctttctt tcaattgcag
     6961 tttttgctcg ccgccaaatt tgaaagcgtc ggagaggtaa tttttgccgg tttcgccgtc
     7021 gctgagcgcc agttgccact catcgcccgc cggataaaga ccaaaattat agattttgcc
     7081 tgcttgccag gagcgatcta gcaacacttc ctgcgcacgt tcaaaagtca gctggttagt
     7141 gctgctgtag ttggtgaagg caatggcgat ggtgcagacc agagggaaga ggacgaataa
     7201 tcccattcca gccattcccg ggtaaacata gcgccaggcg taggctttac gattggcgaa
     7261 aatatacagc cccgctgagc tcaatatcag cgtggtaatg gcgaacaggt attccccttg
     7321 tgcgtacatt aaaacaacaa ggtaacccac cagcaggccg agcagaccta acactgacca
     7381 tttcagcgcg tcgctttgcc accaatgttt ctttttaatg acatccatgg ggttcttcct
     7441 cattccagga cggataaggc tttcatacct tatccgacaa caactgcctg atgcgacgct
     7501 gacgcgtctt atcaggccta catacgtttc aatttcgtag gccggataag gcgttcacgc
     7561 cgcatccggc atttcacagc attacttggt gatacgagtc tgtgcatctt tcagcgcagc
     7621 atccacagtc tgacgaccgc tggcggcgtt gatcaccgca gtacgcacgg cataccagaa
     7681 agcggacatc tgcgggatgt tcggcataat ttcacctttc tgagcgttat ccatggtggc
     7741 ggcaatacgt ggatctttcg caagttcatc ctgataggac tttagcgcta ctgcacccag
     7801 cggtttgtct ttattgaccg cttccagacc ttcgtcagtc agcagatagt tttcgaggaa
     7861 ctctttcgcc agctctttgt tcggactggc ggcgttaata cctgcgctca gcacgccaac
     7921 gaacggttta gatggttgac ctttgaaggt tggcagtacc gttacaccat aattcacttt
     7981 actggtgtcg atgttggacc atgcccacgg gccgttgata gtcatcgctg tttcgccttt
     8041 attaaaggca gcttctgcga tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat
     8101 cagatcaacc aggaaggtca gacccgcttt cgcgccagcg ttatccacgc ctacgtcttt
     8161 aatgtcgtac ttgccgtttt catacttgaa cgcataaccc ccgtcagccg caattagcgg
     8221 ccaggtgaag tatggttctt gcaggttgaa catcagcgcg ctcttacctt tcgctttcag
     8281 ttctttatcc agcgccggga tctcttccca ggtttttggc gggttcggca gcagatcttt
     8341 gttataaatc agtgataacg cttcgacagc gatcgggtaa gcaatcagct tgccgttgta
     8401 acgtacggca tcccaggtaa acggatacag cttgtcctgg aacgctttgt ccggggtgat
     8461 ttcagccaac aggccagatt gagcgtaacc accaaagcgg tcgtgcgccc agaagataat
     8521 gtcagggcca tcgccagttg ccgcaacctg agggaatttc tcttccagtt tatccggatg
     8581 ctcaacggtg actttaattc cggtatcttt ctcgaatttc ttaccgactt cagcgagacc
     8641 gttatagcct ttatcgccgt taatccagat taccagttta ccttcttcga ttttggcgag
     8701 agccgaggcg gaaaacatca tcgtcgttaa tgcggataat gcgaggatgc gtgcacctgt
     8761 ttttattttc ataatctatg gtccttgttg gtgaagtgct cgtgaaaaca cctaaacgga
     8821 ctctagtttc tttatacggc aacctctttc catcctcctt gcccctacgc cccatcgttg
     8881 ctttgtgtga tctctgttac agaattggcg gtaatgtgga gatgcgcaca taaaatcgcc
     8941 atgatttttg caagcaacat cacgaaattc cttacatgac ctcggtttag ttcacagaag
     9001 ctgcgttctc atcctcccgc ctcctccccc ataaaaaagc cagggggtgg aggatttaag
     9061 ccatctcctg atgacgcata gtcagcccat catgaatgtt gcagtcgatg acaggttgtt
     9121 acaaagggag aagggcatgg cgagcgtaca gctgcaaaat gtaacgaaag cctggggcga
     9181 ggtcgtggta tcgaaagata tcaatctcga tatccatgaa ggtgaattcg tggtgtttgt
     9241 cggaccgtct ggctgcggta aatcgacttt actgcgcatg attgccgggc ttgagacgat
     9301 caccagcggc gacctgttca tcggtgagaa gcggatgaat gacactccgc ctgcagaacg
     9361 cggcgttggt atggtgtttc agtcttacgc gctctatccc cacctgtcag tagcagaaaa
     9421 catgtcattt ggcctgaaac tggctggtgc gaaaaaagaa gtgattaacc agcgagttaa
     9481 ccaggttgcg gaagtgctgc aactggcgca tttgctggat cgcaaaccga aagcgctctc
     9541 cggtggtcag cgtcagcgtg tggcgattgg ccgtacgctg gtggccgagc caagcgtatt
     9601 tttgctcgat gaaccgctct ccaacctcga tgctgcactg cgtgtgcaaa tgcgtatcga
     9661 aatctcccgt ctgcataaac gcctgggccg cacaatgatt tacgtcaccc acgatcaggt
     9721 cgaagcgatg acgctggccg acaaaatcgt ggtgctggac gctggtcgcg tggcgcaggt
     9781 tgggaaaccg ctggagctgt atcactatcc ggcagaccgt tttgtcgccg gatttatcgg
     9841 ttctccaaag atgaacttcc tgccagtaaa agtgaccgcc accgcaatcg atcaagtgca
     9901 ggtggagctg ccgatgccga atcgtcagca agtctggcta ccggtcgaaa gccgtgatgt
     9961 ccaggttgga gccaatatgt cactgggtat tcgcccggaa catctactgc cgagtgatat
    10021 cgctgacgtc atccttgagg gtgaagttca ggtcgtcgag caactcggca acgaaacgca
    10081 aatccatatc cagatccctt ccattcgtca aaacctggtg taccgccaga acgacgtggt
    10141 gttggtagaa gaaggtgcca cattcgctat cggcctgccg ccagagcgtt gccatctgtt
    10201 ccgtgaggat ggcactgcat gtcgtcgact gcataaggag ccgggcgttt aagcacccca
    10261 caaaacacac aaagcctgtc acaggtgatg tgaaaaaaga aaagcaatga ctcaggagat
    10321 agaatgatga ttactctgcg caaacttcct ctggcggttg ccgtcgcagc gggcgtaatg
    10381 tctgctcagg caatggctgt tgatttccac ggctatgcac gttccggtat tggctggaca
    10441 ggtagcggcg gcgaacaaca gtgtttccag actaccggtg ctcaaagtaa ataccgtctt
    10501 ggcaacgaat gtgaaactta tgctgaatta aaattgggtc aggaagtgtg gaaagagggc
    10561 gataagagct tctatttcga cactaacgtg gcctattccg tcgcgcaaca gaatgactgg
    10621 gaagctaccg atccggcctt ccgtgaagca aacgtgcagg gtaaaaacct gatcgaatgg
    10681 ctgccaggct ccaccatctg ggcaggtaag cgcttctacc aacgtcatga cgttcatatg
    10741 atcgacttct actactggga tatttctggt cctggtgccg gtctggaaaa catcgatgtt
    10801 ggcttcggta aactctctct ggcagcaacc cgctcctctg aagcgggtgg ttcttcctct
    10861 ttcgccagca acaatattta tgactatacc aacgaaaccg cgaacgacgt tttcgatgtg
    10921 cgtttagcgc agatggaaat caacccgggc ggcacattag aactgggtgt cgactacggt
    10981 cgtgccaacc tgcgtgataa ctatcgtctg gttgatggcg catcgaaaga cggctggtta
    11041 ttcactgctg aacatactca gagtgtcctg aagggtttta acaagtttgt tgttcagtac
    11101 gctactgact cgatgacctc gcagggtaaa ggtctgtcgc agggttctgg cgtcgcgttt
    11161 gataacgaaa aatttgccta caatatcaat aacaacggtc acatgctgcg tatcctcgac
    11221 cacggtgcga tctccatggg cgacaactgg gacatgatgt acgtgggtat gtaccaggat
    11281 atcaactggg ataacgacaa cggcaccaag tggtggaccg tcggtattcg cccgatgtac
    11341 aagtggacgc caatcatgag caccgtgatg gaaatcggct acgacaacgt cgaatcccag
    11401 cgcaccggcg acaagaacaa tcagtacaaa attacccttg cacaacaatg gcaggctggc
    11461 gacagcatct ggtcacgccc ggctattcgt gtcttcgcaa cctacgccaa gtgggatgag
    11521 aaatggggtt atgactacac cggtagctct tcaaccaacc catactacgg caaggctgtg
    11581 tcggctgatt tcaacggcgg tagcttcggt cgtggcgaca gcgacgagtg gaccttcggt
    11641 gcccagatgg aaatctggtg gtaatagcaa aacctgggcc ggataaggtg cttgcgccgc
    11701 atccggcaac caatgcctga tgcgacgctg ttgcgtctta tcaggcctac aactgctgcc
    11761 gaaagtaggc cggataaggc gtttacgccg catccggcat aaaaacagct ttaaggggcg
    11821 aaagcccctc tgattatcgg gtttagcgcg ctattgcctg gctaccgctg agctccagat
    11881 tttgaggtga aaacaatgaa aatgaataaa agtctcatcg ctctctgttt atcagcaggg
    11941 ttgctggcaa gcgcacctgg aatcagcctt gccgatgtta actacgtacc gcaaaacacc
    12001 agcgacgcgc cagccattcc atctgccgcg ctgcaacaac tcacctggac accggtcgat
    12061 caatctaaaa cccagactac tcaactgtcg accggcggcc aacaactgaa tgtccctggc
    12121 atcagtggtc cggttgctgc gtacagcgtc ccggcaaaca ttggtgaact gacactgacg
    12181 ctgaccagcg aagtgaacaa acaaaccagc gtatttgcgc cgaatgtgct gattcttgat
    12241 cagaacatga ccccatcagc cttcttcccc agcagttatt tcacctacca ggaacccggc
    12301 gtgatgagtg cagatcggct ggaaggcgtt atgcgcctga caccggcgtt ggggcagcaa
    12361 aaactttatg ttctggtctt taccacggaa aaagatctcc agcagacgac tcaactgctc
    12421 gacccggcta aagcctatgc caaaggcgtc ggtaactcga tcccggatat tcccgatccg
    12481 gttgctcgtc ataccaccga tggcttactg aaactgaaag tgaaaacgaa ctccagctcc
    12541 agcgtgttgg taggaccact atttagttct tccgctccag ctccggttac ggtaggcaac
    12601 acggcggcac cagctgtggc tgcacccgct ccggcaccgg tgaagaaaag cgagccgatg
    12661 ctcaacgaca cggaaagtta ttttaatacc gcgatcaaaa acgctgtcgc gaaaggtgat
    12721 gttgataagg cgttaaaact gcttgatgaa gctgaacgcc tggggtcgac atctgcccgt
    12781 tccaccttta tcagcagtgt aaaaggcaag gggtaattac gccccacagt gctgattttg
    12841 caacaactgg tgcgtctcct ggcgcacctt tttttctcct cctgtactat tgttgcgttt
    12901 ttgttgcgca ttagttcact taatttgctt tacatctccc gtaaatattt ttctgcgata
    12961 caatgccttt acgttatgta acggagagtt cggcatgtca caccccgcgt taacgcaact
    13021 gcgtgcgctg cgctatttta cagagatccc tgccctggag ccgcaactgc tcgactggct
    13081 gttgctggaa gattccatga caaaacgttt tgaacagcag ggaaaaacgg tgagcgtgac
    13141 gatgatccgc gaagggtttg tcgagcagaa tgaaatcccc gaagaactgc cactgctgcc
    13201 gaaagagtct cgttactggt tacgtgaaat tttgttatgt gccgatggtg aaccgtggct
    13261 tgccggtcgt accgtcgttc ctgtgtcaac gttaagcggg ccggagctgg cgttacaaaa
    13321 attaggtaaa acgccgttag gacgctatct gttcacatca tcgacattaa cccgggactt
    13381 tattgagata ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc gattaagcgg
    13441 taaaccgctg ttgctaacag aactgttttt accggcgtca ccgttgtact aagaggaaaa
    13501 aaatatggag tggagtctga cgcagaataa gctgctggcg tttcatcgct taatgcgtac
    13561 ggataagcca attggcgcgt tactgctgct ctggccaaca ttatgggcgt tgtgggtggc
    13621 gacaccgggc gttccccagc tctggatcct ggcggtgttt gtcgcgggcg tctggctgat
    13681 gcgcgctgct ggttgcgtgg tgaatgatta tgctgaccgc aagtttgatg gtcatgttaa
    13741 gcgcacggcg aaccgaccac ttcccagcgg cgcggtaacg gagaaagagg cgcgcgcgct
    13801 gtttgtagtg ctggtactga tttcattttt actggtgctg acgctgaata cgatgactat
    13861 tctgttgtcg attgccgcgc tggcgctggc gtgggtgtac ccgtttatga agcggtatac
    13921 ccatctaccg caagtggtgc tgggcgcggc gtttggctgg tcgattccaa tggcttttgc
    13981 cgctgtgagt gagtcggtgc cattgagttg ctggttaatg ttcctcgcca atattctctg
    14041 ggcggtggct tacgacacgc agtatgcgat ggttgaccgc gatgatgatg tgaagattgg
    14101 cattaaatcc accgcaatcc tgttcggcca atacgataaa ttgattattg gtattttgca
    14161 gattggcgta ctagcgctga tggcgatcat cggtgagtta aatggcttag gctggggata
    14221 ttactggtca attctggtag ctggcgcgct gtttgtttat caacaaaaac tgattgccaa
    14281 ccgcgagcgt gaagcctgct ttaaagcatt tatgaataat aactatgttg gtctggtact
    14341 gtttttaggg ctggcaatga gttactggca tttctgatga tgtaaaaaag ccggatgatc
    14401 atccggcttt cttctgggtt gcctgaggcg ctgcgcttct ctggcctata caacatatcg
    14461 caatttattg aatttgcagg tcatggtagg ccgaataagg cgtttgcgcc gcatccggca
    14521 attctcactg attacccttc gccctgcgtc gcactctcaa tcgtcaaacg cacgtctgat
    14581 gtaatcaact ctgccagcaa ctgataaacc ttcatcgttt ctgccggttc ggcgtcgccg
    14641 ctatcgctga tatacccttc atcacgcagc gtcagcacca gagaactgaa caccgccttg
    14701 tcgaagaact ccggcgcgtt gataccgtgc agcacggaga gacgttgcgc gacggtgcgg
    14761 ctctcttttt ccagcgtacc gcggttgatc gacgggttgg cactcaacag ccagaaggtg
    14821 atggcataac gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag cgtgcgagaa
    14881 tgcgccgggt tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg acgttgcatc
    14941 tcatttgcca gcgcatcaat aacgtccggt aactcgtcgc gatcccagcg caggaacagt
    15001 tccgctttca gcattgggta aagcacattg acgtgctcca tcaatacgtc gcgggagatg
    15061 tggcgatgct gggtgacaat tgccgccatc agcgaaggca gcaccaacat atgcgcaatg
    15121 ttgttgcgat agtaggtcat cagcaccgct tgctcgcgcg gcagaatgat gatgtcgccg
    15181 atagtgtctt tctcaacttc aaacttgttc atttgcagcg cgtgatcgat aagctcgctg
    15241 gcgctggctg aaggaacggt agagtccgtg gagtaaggta cgttgcgcat caaatccagg
    15301 tagcagttga gttgctcggt taactgctcg cgggtgagtg agcgctgacg tgacgccaga
    15361 agcgcagtac agcacaggtt catggcgttt gccgcgcctg cgttgttaat gcgtaccatc
    15421 agatcggcag caatattatt gaccgtcggc gttaaccagg ccggacgcac cgcttcgatg
    15481 ggatcgatag attcacgcca gtctggtacg tgctggttaa ggtaggtcat caacggcatt
    15541 ggttcaccga agttgacgta accctgaccg agattacgca gcttgcttaa accgcgcagc
    15601 atctgcggca ggctctcttt ctctttcgtc gcaccgcgca gttctttggc gtaagtaccc
    15661 acttccatga cgtgctcata accgatatag atcggaatca gcgtaatcgg acgagtgccg
    15721 ccacgcagca tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc cagcaaacgc
    15781 cccgtacggg aacgaccgcc ttccacgaaa tactcgacgg aataaccacg gctgaacagt
    15841 tcgccgagat actcacggaa aacggtggaa taaagtttat tgcctttaaa cgtacggcga
    15901 ataaagaacg cccccagacg gcggaaaatc ggcccggccg gccagaagtt caggttgatc
    15961 ccggcggcga tatgcggcgg caccagcccc tggtgataca gcacgtaaga aagcagcagg
    16021 tagtccatgt gactgcggtg gcaaggcaca tacactagct catggccgtc gtgggccagc
    16081 tggcgaacgc gctcggcgtt atggacgttg ataccctggt aaagtcggtt ccaggtgaag
    16141 cccagaatgc ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc aatctcttcc
    16201 atcagcgcaa tcgcgttctg ctgcgctttt tcatgggaga tttttttgct gcgtgcttca
    16261 tcttctaccg ctttcgcaat ggcgcgggag gcgagcagct tattaaacag atcctgacga
    16321 gcaggcagac gtggacccac ggcagccaga cgttgacggg caaagtgcat acgcgccacg
    16381 cgcgccagtt tctgagcgat agttttatcc gtgccgtgtt catccgccat acggcgcagc
    16441 gaaactgacg gcgagaaacg cacaaaactg tcgcgaccga gccacagtac agcgaaaaat
    16501 ttctgtacgc cgttaagcat acgcagcggc gggttcactt cgcctttttc acgccccggc
    16561 gcgcgaccaa acatcaccga tactggcacc atctgcacat ccagatttgg gttgctgcgg
    16621 tgcaaatcga gatagtcgtg gaacagctta atagactctt ctttcggcgt gtaataggtg
    16681 aacacacgcg gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc gtcgatttcc
    16741 agcggctcta acgggtcagg caggtcatgt gccagacact gggcgcgcaa cgtcagcaag
    16801 tcagctttcg agttgtacgg caaaacgtac ataattggac gagaggtatc cagccccagt
    16861 tccggggcag gatccgccgg aatagacttg ctttttacca ggatgcttaa tggtaaattc
    16921 agtaatttgt agtaaattcg tggccagccg gacataaacg atgtaaagcc tctggttaat
    16981 aatgcaaatg cgcggcaagg atagcagaaa gtcatgggaa attctgtggt atccgctcat
    17041 gtttcgcgcg gcgttacgca aacccgaatc atcggattta acggtacact gatattgacg
    17101 ctcataatgt aaaaaggtta tttcaatggc caataatacc actggattca tccgaattat
    17161 caaagctgct ggctattcct ggaaaggttt acgcgctgca tggatcaacg aagcggcatt
    17221 ccgtcaggaa ggcgtagcgg tattgttagc ggtggttatc gcctgctggc tggatgtgga
    17281 cgcgattacc cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg tggaaattct
    17341 caatagcgcc atcgaagctg tggttgaccg aattggctct gaataccatg agctttccgg
    17401 acgcgctaaa gatatgggat ccgccgcagt gctgattgcc attatcgtcg ccgtgattac
    17461 ctggtgcatc ctgttatggt cgcattttgg ataacccttc cagatttcga taaatctctg
    17521 gtttattgtg cagtttatgg ttccaaaatc gccttttgct gtatatactc acagcataac
    17581 tgtatataca cccagggggc ggaatgaaag cgttaacggc caggcaacaa gaggtgtttg
    17641 atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg gaaatcgcgc
    17701 agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg ctggcacgca
    17761 aaggcgttat tgaaattgtt tccggcgcat cacgcgggat tcgtttattg caggaagagg
    17821 aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accgcttctg gcgcaacagc
    17881 atatcgaagg tcattatcag gtcgatcctt ccttgttcaa gccgaatgct gatttcctgc
    17941 tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggcgat ttgctggcag
    18001 tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt gatgacgaag
    18061 ttaccgttaa acgcctgaaa aaacagggca ataaagtcga actgttgcca gaaaacagcg
    18121 agtttaaacc aattgtcgtt gaccttcgtc agcagagctt caccattgag gggctggcgg
    18181 ttggggttat tcgcaacggc gactggctgt aacatatctc tgagaccgcg atgccgcctg
    18241 gcgtcgcggt ttgttttttc atctctcttc atcaggcttg tctgcatggc attcctcact
    18301 tcatctgata aagcactctg gcatctcgcc ttacccatga ttttctccaa tatcaccgtt
    18361 ccgttgctgg gactggtcga tacggcggta attggtcatc ttgatagtcc ggtttatttg
    18421 ggcggcgtgg cggttggtgc aacggcgacc agctttctct ttatgttgtt gctgttttta
    18481 cgcatgagca ccaccgggct gactgcgcag gcttatggtg ccaaaaatcc tcaggcacta
    18541 gcccgtgcgc tggtgcaacc gttgctgttg gcgttggggg ccggggcgtt aattgcgctg
    18601 ctgcgtacgc cgattatcga tctggcgctg catattgttg gtggtagtga agcagtcctg
    18661 gaacaggcgc gacgctttct tgaaatccgc tggttaagcg caccggcgtc gctggcgaat
    18721 ctggtattac ttggctggtt actcggcgtg caatatgccc gtgcgccagt aattttgtta
    18781 gtggtcggca atatcctcaa cattgtgctg gatgtctggc tggtgatggg gctgcatatg
    18841 aacgtgcagg gcgcggctct ggcaacggtt attgcggaat atgcaacatt gctgattggt
    18901 ctgctaatgg tgcgtaaaat cctcaaacta cgcggaatct ccggcgaaat gctgaaaaca
    18961 gcctggcgag gaaacttccg tcacttgctg gcgcttaacc gcgatatcat gctgcgctcg
    19021 ctgttgctgc aactctgttt cggcgcgatc actgtacttg gcgcgcgact ggggagtgac
    19081 attatcgctg ttaacgcggt tctgatgacg ctactcacct ttaccgccta tgcgctggat
    19141 ggttttgcct acgcggttga agcacattcc ggtcaggcat acggtgcgcg cgacggtagt
    19201 caattgctgg atgtctggcg ggcagcgtgc cgccagtcgg ggatcgtagc gttactgttt
    19261 tcggtggttt atttgctggc aggggaacac atcattgcgt tgctgacgtc gttaacccag
    19321 attcagcagc tggctgaccg ctatcttatc tggcaggtga ttttgccgtt ggtcggcgtc
    19381 tggtgttatc tgctggacgg catgtttata ggcgcaacgc gtgccgccga aatgcgtaac
    19441 agtatggcgg tggccgccgc aggttttgca ctgacgctcc ttacgctgcc gtggctgggg
    19501 aatcatggtt tgtggctggc gttaaccgtc tttctggcgt tacgcgggct ttctctggcg
    19561 gctatctggc ggcgtcactg gcgtaacgat acctggtttg tcgcaacgtg acggttaaaa
    19621 attctgaata aataatccta agccaaattg ctgactacac ttaatctcac gttcagaaga
    19681 aaagtgaacg tactctcatt cacaacctaa cgatgaggtc ttgattatga ataaagatga
    19741 agccggcggt aactggaaac agtttaaagg taaagtgaaa gagcaatggg gcaaactgac
    19801 cgatgatgat atgacgatca ttgaaggaaa acgtgatcag ttagtgggta aaatccagga
    19861 acgctacggt tatcagaaag atcaggcaga aaaagaggtt gatagttggg aaaaacgcca
    19921 tgattaccgc tggtaattaa tccctcctgc ccgacatgta caaggatgta caccctttta
    19981 gcgaggtttc tttttcacct gaatagagtg gtcatgatgg cactgttcag gatgacgaca
    20041 cgcttctact tctacacatg ctgaacataa tccatgtgct tcaataacat tatgccgcag
    20101 ggcaaaaccc atttttgccg ccagcgtatg catgatgtct tccacgcctt cagcacattc
    20161 ttctttcacc gcgccgcagc gatcgcaaat aaacatggct gaagtatggg tgggctgatc
    20221 gaacagatga cagagcacat aactgttggt ggattccacc ttatgcacaa aaccttgttc
    20281 aagcagaaac tccagcgcgc gataaaccgt tggcggcttg gcttgcggtt cagcttcgcg
    20341 cagcaaatca agcagatcat aagcgctgat agcgccatcc tgcagactca tcaggcgcaa
    20401 cacttcgaga cgctgtgggg tcaggcgcac attacgctgt gcgcagattt tttcagcctg
    20461 cgctaataac tcctgcgttg tggtcttttc cattgggcac ctcgacagtg gggttaagaa
    20521 tgtccttact ttaccatagt tctcacttaa acgccgagaa cagggtgtca tggctgtcac
    20581 attgcctgat cgcatattcg ttatgctata gtagcgcccc caatttacca gatgcttaaa
    20641 aagtcgccat gccagcagaa tcacacactg tttatcccgc ttaccgtttc tcgattgccc
    20701 cgatgcttga ctggacggac agacattgcc gttatttctt gcgtctgctt tcccgcaata
    20761 cgttgctgta taccgaaatg gtgaccacag gggcgattat tcacggtaaa ggtgattacc
    20821 tggcgtacag tgaagaagaa catccggtag cgttgcaact cgggggtagc gatccggcgg
    20881 cgctggcaca gtgtgcgaag ctggcagaag cgcgtggata cgatgagatc aacctgaatg
    20941 tcggctgccc gtctgaccgg gtgcagaacg gcatgtttgg tgcgtgtctg atgggtaatg
    21001 cgcagctggt tgccgactgc gtgaaagcga tgcgcgatgt ggtgtcgatt ccggtgacgg
    21061 tgaaaacgcg tattggcatc gatgatcagg acagctatga atttctctgc gatttcatca
    21121 acaccgtttc cggcaaaggt gagtgtgaga tgttcatcat ccacgcacgt aaagcctggc
    21181 tttctgggtt aagtccgaaa gaaaaccgtg agatcccgcc tctcgattat ccgcgtgtgt
    21241 atcaactgaa gcgtgacttt ccgcatctga cgatgtcgat taacggtggt atcaagtcgc
    21301 tggaagaagc taaagcgcat ttgcaacata tggatggcgt gatggtcggg cgcgaggcgt
    21361 atcagaatcc ggggattctg gcggcggtag accgagagat ttttggttcc tcggataccg
    21421 atgccgatcc ggtggcggta gtgcgcgcca tgtatccgta cattgagcgt gaactcagcc
    21481 agggtacgta tctcggccat attacccggc atatgctggg cttgttccag ggtattcctg
    21541 gcgcgcggca gtggcggcgt tatttaagtg aaaatgccca taaagcgggt gcagacatta
    21601 atgtgctgga acacgcgctc aaactggtgg cggataagcg ttaacttttc accaaaaagt
    21661 agtcaaattc accacgccct gcgccccgtc gcggggcgtt ttgctgttaa atcaatagat
    21721 tatttttggc atgattcttg taatgccagc aagagatttc atatttggga gagcatcatg
    21781 ctggaactac tttttgtaat tggctttttt gtcatgctga tggtcaccgg tgtttcgttg
    21841 ctgggtatta tcgccgcgct gattgtggcg acggccttta tgttcctcgg cggtatgctg
    21901 gcattgatga ttaagttgct gccgtggctg ctgttggcgg ttgcggtggt gtgggtgatc
    21961 agggcgataa aagcaccaaa agtgccgaaa tatcagcgtt atgaccgctg gcgttactaa
    22021 gggattgtgc ggatgatcac aacctaaggt tttatcctta gaacaaaata ggaattgata
    22081 atcaaatctg tcactattgc gcgtcaaaca aattcatcga gctgtaccct acatacagcc
    22141 gaactataaa agaaagggct tcccaggtgg aagcccaatt tcttttatgg aatcaatagg
    22201 ctggaaccct gcgtcgcccg gctttccaga atctcatgcg cacgccgcgc atccttcagc
    22261 ggatatttct gctgctcggc gacatcgacc ttaatcacac cgctggcaat caaagagaac
    22321 agttcattac tggcctcggt taattcctcc cgcgtggtga tatagccttg cagggaaggg
    22381 cgtgtcacat acaacgagcc tttctgattg agaatgccta agttcacacc ggtaaccgca
    22441 cctgatgagt tgccaaaact caccattaag ccgcggcgtt gcaggcaatc cagcgaccgt
    22501 tcccaggtgt ctctgcccac tgaatcgtac accacgcgca ctttcttacc accggtgatc
    22561 tctttcaatc gctcgaccag attctcttca cgatagttaa taacctgcca cgcgcccgct
    22621 tttagcgcac tctgcgcttt ttgcgcggtt cctacagtgc cgataagttt cgcgcccagg
    22681 gcttttgccc actggcaggc aatcaagcca acgccgccag ccgctgcgtg gaacaggaac
    22741 tgttcatcgg gtttaatttc ataggttttg cgcagcagat aataaaccgt taagcctttc
    22801 aggaaggatg ccgcagcttg ctcaaaagaa attgccgcag gcagaatcgc cgctttatct
    22861 gcattaatgt tatgcacaga gctgtaagcg cctaatgccg attgtgcata gactacgcga
    22921 tcgcctgcct taatatgctt tacaccactg ccgactttac tcacgatgcc tgccgcttcg
    22981 gtacctaatc cgctgggtag cgatggcggc gggtaaaggc cgctgcggat atacgtgtcg
    23041 ataaaattga tgccgatggc tttattttcg acctggattt cattctccgc cggatcggca
    23101 ggagtgaact ctacggcttg aagtacttcc gggccaccgt gcttgtgaaa ttcaattcgt
    23161 gttgccatgt gtcctccaga acgtatcgtc agggtctgct tcatatggta aagtttcgac
    23221 ccattcttta tctcggtaac tccattcact atggcaggaa ataaaccctt caacaaacag
    23281 caggctgaac cccgcgaacg cgatccacaa gttgccgggc tgaaagtgcc tccgcactcg
    23341 atcgaagcgg agcagtcggt gttgggcggt ttaatgctgg ataacgaacg ctgggatgat
    23401 gtagctgagc gtgtggtggc agatgatttt tacacccgcc cacaccgtca tatctttact
    23461 gaaatggcgc gtttgcagga aagcggtagt cctatcgatc tgattaccct tgcggaatcg
    23521 ctggaacgcc aggggcaact cgatagcgtc ggtggttttg cttatctggc agagctgtca
    23581 aaaaatacgc caagtgcggc gaacatcagt gcatatgcgg atatcgtgcg cgaacgtgcc
    23641 gttgttcgtg agatgatctc cgttgctaac gagattgccg aagctggttt tgatccacaa
    23701 gggcgtacca gtgaagatct gctcgacctt gctgaatccc gcgtctttaa aattgccgaa
    23761 agtcgtgcaa acaaagacga agggccgaag aacatcgccg atgtgctcga cgcaaccgtg
    23821 gcgcgtattg agcagttgtt tcagcagcca cacgatggcg ttaccggggt aaataccggt
    23881 tatgacgatc tcaacaaaaa aaccgctggc ttgcagccgt cggatttgat catcgttgcc
    23941 gcgcgtccgt cgatgggtaa aacgacattt gcgatgaacc tcgtcgaaaa cgcggcgatg
    24001 ttgcaggata aaccggtgct tatcttctcg ctggagatgc catcagaaca gatcatgatg
    24061 cgttctctgg cgtcgctgtc gcgcgttgac cagactaaaa tccgtaccgg gcagctcgat
    24121 gatgaagact gggcgcgcat ttccggcacc atgggtattt tgctcgaaaa acgtaatatc
    24181 tatatcgatg attcctccgg cttgacgcca acggaagtgc gttcccgcgc acgccgtatt
    24241 gcccgtgaac acggcggcat cgggcttatc atgatcgact acctgcaact gatgcgcgta
    24301 ccggcgcttt ccgataaccg tacgctggaa attgcagaaa tctcccgctc gctgaaagca
    24361 ctggcgaaag aactgaacgt gccggtggtg gcgctgtccc agttgaaccg ttctctggaa
    24421 caacgtgccg acaaacgccc ggtcaactcc gacctgcgtg aatctggctc tatcgagcag
    24481 gatgcggact tgatcatgtt tatctatcgt gatgaggtgt atcacgaaaa cagtgattta
    24541 aaaggcatcg cggaaattat tatcggtaaa caacgtaacg gcccaatcgg gacggtacgc
    24601 ctgaccttta acggtcaatg gtcgcgcttc gacaactatg cggggccgca gtacgacgac
    24661 gaataataat tattttatga attaggtaat taaagcaaac acttatcaag gaacacaaat
    24721 gcaagcggca actgttgtga ttaaccgccg cgctctgcga cacaacctgc aacgtcttcg
    24781 tgaactggcg cctgccagta aaatggttgc ggtggtgaaa gcgaacgctt atggtcacgg
    24841 tcttcttgag accgcgcgaa cgctccccga tgctgacgcc tttggcgtag cccgtctcga
    24901 agaagctctg cgactgcgtg cggggggaat caccaaacct gtacttttac tcgaaggctt
    24961 ttttgatgcc agagatctac cgacgatttc tgcgcaacat tttcataccg ccgtgcataa
    25021 cgaagaacag ctggctgcgc tggaagaggc tagcctgaac gagccggtta ccgtctggat
    25081 gaaactcgat accggtatgc accgtctggg cgtaaggccg gaacaggctg aggcgtttta
    25141 tcatcgtctg acccagtgca aaaacgttcg tcagccggtg aatatcgtca gccattttgc
    25201 gcgcgcggat gaaccaaaat gtggcgcaac cgagaagcaa ctcgctatct ttaatacctt
    25261 ttgcgaaggc aagcctggtc aacgttccat tgccgcgtcg ggtggcattt tactctggcc
    25321 acagtcgcat tttgactggg tgcgcccggg catcattctt tacggcgtat ctccgctgga
    25381 agatcgctcc accggtgccg attttggctg tcagccagtg atgtcgctaa cctccagcct
    25441 gattgccgtg cgtgagcata aagccggaga gcctgtcggt tatggtggaa cctgggtaag
    25501 cgaacgtgat actcgtcttg gcgtagtcgc gatgggctat ggcgatggtt atccgcgcgc
    25561 cgcgccgtcc ggtacgccag tgctggtgaa cggtcgcgaa gtgccgattg tcgggcgagt
    25621 cgcgatggat atgatctgcg tagacttagg tccacaggcg caggacaaag ccggggaccc
    25681 ggtcatttta tggggcgaag gtttgcccgt agaacgtatc gctgaaatga cgaaagtaag
    25741 cgcttacgaa cttatcacgc gcctgacctc aagagtcgcg atgaaatacg tggattaata
    25801 ttcgtctcca gggagggaca atgtcttatt cagaaatcgc cgcgtgtctg gcatacgcgg
    25861 tttccgtctg gctggctgcc agaaacaatg tgcatacatg gtggatcggc ataattggca
    25921 gcatattgta cggctgggtt ttttggtccg tgcaactcta tgccgacgtt acgctccagt
    25981 tattcttcat cgcgaccagc atcactggct ggatccactg gctgaaaggt cagggtggcg
    26041 acatcttgcc ggtgcgccga acgcaagcca gtcacttttt ccttttgttg ctctgtgctg
    26101 tcgtcgtggc aggcggttac ggctttttac tccacacctt taccaatgcc tgggcaccct
    26161 ggctggattc gttgattttg acctttagcg ttctggcaca attcatgttg atgggaagac
    26221 gtatcgaaaa ctggttcgtc tggttagcgg tgaataccct ggcagtgcca ctgtatatga
    26281 cgcgcggttt aaacctgacc gctggcttat atttcctgtt ctggattaac gcctggcatg
    26341 gtttgtatca atggcgcaaa gagttgcaaa catcatgaag ccttttgcaa cgggcctggt
    26401 ggttggtaaa ttcgcgcctt tgcattgtgg tcatgaaaag ttgatcaata ccgcactggc
    26461 gcaatgtgaa gaactgttca tcatcagtta ttccgtgccg gaaatgcctg actgtgaacc
    26521 tgaaaagcgc ctgacgtggc tgcaagtgcg cttcccgcaa gcgacgattt tggtactgac
    26581 gccagagctt gttgcgcgtt ataacttacc tgccataccg cataacgatg ccgatgccga
    26641 catccaccgt cattatgtgg cgacgctgtg cctgcagata ttacgctgtc gtccacatgc
    26701 tgtatttact gcagaagact atggcgatgg ctttgctaac gtgctggcgc ggcgttttgc
    26761 ccaacccgtt gaacacgttc gaatggcgcg cccagttggc gatgaagcgc cttcgggaac
    26821 gcttattcgt tccgatgttc accgctatcg ctacatgttg gcgaatgatg tttattacag
    26881 tttcgttcgc cgtatctgtc tgcttggtgg cgaatctacc ggtaaaagca cgctttcaaa
    26941 agcgctggcg gatgggcttg atacggtgta tgtcgctgaa tttggccgcg actattggga
    27001 agaaaaaaac ggaatcttaa ccgcggatga tttgttgcat atcgcctgcg aacaggtacg
    27061 ccgggaacaa caagcagaag ctaatcacta tcttatatgt gacacgtcgc cactcacgac
    27121 cttgttctat gcccttgatc aatatggtca tgctccgcaa gaacttcatc aactggcgga
    27181 gcgggaatac tctgtggtgg tgctttgcgg cgcagaattt cccttcgtgc aggatggcac
    27241 ccggcaagga gaggtatttc gcgcacggca acaggtgtgg tatgaacagg aattgtcgcg
    27301 caggaacatt ccttatcttt cggtttcggg gccactgcaa gagcgagtgg agcaaattct
    27361 tcgccaactt cccgactgac tttgaacatc ctctcgatct tcgccttctt ccggtttatt
    27421 gtgttttaac cacctgcccg taaacctgga gaaccatcgc gtgtttcaaa aagttgacgc
    27481 ctacgctggc gacccgattc ttacgcttat ggagcgtttt aaagaagacc ctcgcagcga
    27541 caaagtgaat ttaagtatcg gtctgtacta caacgaagac ggaattattc cacaactgaa
    27601 agccgtggcg gatgcggaag cgcgcctgaa tgcgcagcct catggtgctt cgctttattt
    27661 accgatggaa gggcttaaca gctatcgcca tgccattgcg ccgctgctgt ttggtgcgga
    27721 ccatccggta ctgcaacaac agcgcgtagc aaccattcaa acccttggcg gctcaggggc
    27781 attgaaagtg ggcgcagatt tcctgaaacg ctacttcccg gaatcaggcg tctgggtcag
    27841 cgatcctacc tgggaaaacc acgtagcaat attcgccggg gctggattcg aagtaagtac
    27901 ttacccctgg tataacgaag cgactaacgg cgtgcgcttt aatgacctgt tggcgacgct
    27961 gaaaacatta cctgcccgca gtattgtgtt gctgcatcca tgttgccaca acccaacggg
    28021 tgccgatctc actaatgacc agtgggattc cgtgattgaa attctcaaag cccgtgagct
    28081 tatcccattt cttgatattg cctatcaagg atttggtgcc ggtatggaag aggatgccta
    28141 cgccattcgc gccattgcca gcgctggatt acccgctctg gtgagcaatt cgttctcgaa
    28201 aattttctcc ctttacggcg agcgcgtcgg cggactttct gttctgtgtg aagatgccga
    28261 agccgcaggc cgcgtactgg ggcaattgaa agcaaccgtt cgccgcaact actccagccc
    28321 gccgaatttt ggtgcgcagg tggtggctgc ggtgctgaat gacgaggcat tgaaagccag
    28381 ctggctggcg gaagtagaag agatgcgtac tcgcattctg gcaatgcgtc aggaactggt
    28441 gaaggtattg agcacagaga tgccagagca caatttcgat tatctgctta atcagcgcgg
    28501 catgttcagt tataccggtt taagaaccgc tcaggttgac cgactacgtg aagaatttgg
    28561 tgtctatctc atcgccagcg gtcgcatgtg tgtcgccggg cttaacgcgc aaaatttgca
    28621 tcgcgtggca aaggcgtttg ctgcggtgat gtgattttaa tcaacaggcg gatctctccg
    28681 cctgttcctg actccagcca tcccctacta ttctgtccct tttttcgccc acttttacat
    28741 catctatttt ctataagttt ttgaaaatta gtgtttttaa ctgttggtac ggattgtgca
    28801 gtaatgcggc tgtctatgac catcaggaga aaaacatgga gaacatgacc tcgcccggca
    28861 cgttgcttag tggagataac gcgacctggc ttgaagatta ctaccagacc tggttgcgta
    28921 cgccggaaca actcccggaa gactggcggc gtttttttct ttcacctgaa ctgactgtcc
    28981 aaagcgtatc cggtgataac aacgtcagcg gtgcgacctt aaaaaaacag gctgcgataa
    29041 tacaactgat caatgcctgg cgcacgcagg ggcatctgcg cgcaaagctt gatccacttg
    29101 gtttgaatcc cccggctgac gttccctccc tcctgcctgg tttttgggga ttaagcgaag
    29161 aagacttgtt gcaggagttt agcgtcacct ttggcgcaca caccacgcaa atgccactta
    29221 agcaactgct taacctgctg gagcaagcct gggcgggtag ccaggcttat gaacttgctc
    29281 atctggaaaa tcgtgaagaa atcaactggt tgttatcacg gattgagagc agcaatgcgc
    29341 cacaagcaga tgtacaaacc tgtattgctc gttttgaaaa actgatggcg gcagagacgt
    29401 tagagcgtta tctgcatacc cgttatgttg ggcagaaacg gttttctctg gaagggggcg
    29461 aaagtgcaat cccggcgctc gatacgctga caaaacgcct gcgtgcgcaa ggtgtggagg
    29521 aaatggtcat tggcatggcg caccggggaa gacttaatgt tctggttaat ctgttgaata
    29581 aagacccggc acagcttttt gctgagtttg aaggcaagca gacgatcggc agtggttcag
    29641 gcgatgtgaa ataccacatg gggtattcca gcaatctgga aacacctgcc ggttcgctgc
    29701 atgtggcgct ggcgtataac ccctcacatc tggagatcgt gaatccggtg gtgctgggcc
    29761 aggtgcgagc caggcaggaa cgccgtggtg aggatggtca ggcaaaagtg gtgggcgtac
    29821 ttattcatgg ggattccgcg ttgggcggcc tcggcgtcaa ccaaaccacg tttaacttat
    29881 cgcagactca gggctacggc actggcggta cgctacatct ggtgattaac aatcagattg
    29941 gcttcaccac ttcccgcttg caggatatgc gttcttcacg gtactgcacc gatattgcca
    30001 aaatggtagc ggccccaatt atccacgtta atggcgatga cgttgacgct gtttgccagg
    30061 taatggaact ggcgtgcgaa tggcgagaca ccttccggcg cgacatcatc atcgatattt
    30121 gctgcttccg taagcacgga cacaacgaaa gcgatgagcc acgtcttacc cagccacaaa
    30181 tgtatcaggc agtggatgct caccctggta cgctcgcccg ttatggtgaa tctcttgccc
    30241 gccgcggcct gttaactcag gcgcaacaag atgagatgac ggcacgctat cgcgactggc
    30301 tggatagctg ccagaaacgt gaaccgcagc ctctcaaacc tgcaattcac tcttttagcg
    30361 ctaactggta tgggttgacc aaccctcact ggagcgcccc ggtatctacg gcattgcctc
    30421 ggcaaaagct ggcagcgtat ggtgaaatca tttctacact gccacccgat gtggtcgcac
    30481 atcctaccat taaacggcaa ctggcgttac gccaggatat ggccgcaggt actcagccaa
    30541 tagactgggg aatggctgaa atgctggcct atgcatcgct tgtggatgcg ggtgttggtg
    30601 tgcgtctttc tggtgaagac tcagggcgtg gcacgttcag tcatcgccat gcagtggtac
    30661 atcatcaaac tgaagcccgt cgttatctgc cgttacagca tatccgcgct ggtcaggcat
    30721 ctttcgatgt ttatgattcg gtgcttaatg aagaagcgct cttagccttt gaatatggct
    30781 attcaacgtc tgcgcctcag caactggtta tttgggaagc acagtttggt gactttgcca
    30841 acggcgctca ggttgctatt gatcaattta tctcgtctgg cgaaaccaag tgggatcgct
    30901 atagcggttt aaccattctt ctgccgcatg gctatgacgg acaagggccg gagcactctt
    30961 ctgcgcgtcc tgagcgctgg ctgcaactgt gcgcagaaaa caacatgcag gtggtgatgc
    31021 ccagtgagtc agcacaaatg ttccatttgt tgcgcggtca ggcattacgc ccaatgcgca
    31081 aaccgttagt gataatgatg agtaagcgtt tacttcgttt taaaggcgct atgagcgagc
    31141 tttgtgaatt cactgatggc gcttataaac cggtaatcac cgatccgcaa ttgcatcagt
    31201 cccagaaagt taagcgcgtt attttgtgta gcggccaggt ctattacgac gtactggaag
    31261 cccgtaagca gcgtgagcat gaagacgaag tggcgattgt tcgcctggag caactctacc
    31321 cgttcccggt cgcagaactg aatgatgttc tggcgagttg gccgaactgc tgcgaatgga
    31381 tttggttgca ggaagagcca gaaaaccagg gcgcgtggcg gcagattcgt catgagctgg
    31441 cggcactgaa aattaacact ccttactggc agtatgcagg ccgcccggca gcggcagctc
    31501 cggcgacagg gtatgggcgt gtacataaac aacaaatcga cgaatttctt gctgctgcgt
    31561 ttgcagatat tcagccatga aaggcgcaca aaaataataa ggagaacatt ttatgatcga
    31621 aattactgtc cctgtattac cggaatctgt cacggaaggc acgctgacaa cctggtgtaa
    31681 acaagaaggc gagcacgtaa aacgcgatga tgtgattgcc gagctggaaa ccgataaagt
    31741 cattctggaa ataccggccc cgcacgatgg cgtgttaagc aatattatcg tcagcgaagg
    31801 tagcacggtc acgtccgcgc aactgttggc gcatcttaag ccgcaagctg tcatagaaga
    31861 aaccgtcaca ccagtcaccg aaaccctggc gatgccttcc gcacggctgg aggcacagcg
    31921 tagtggtgtc gaacttgctg acgttgcggg gagcgggaga aatggacgta tcctgaaaga
    31981 ggatgtacag cgcgttactc ctgcaccagt cattcagcct gaacgggtgg cggagatcgc
    32041 gccagctaaa cccttgacac cgggtgctcg tcaggaacgt cgtgagccga tgtcgcgctt
    32101 acgtcagcgg attgctgagc gtctgctggc ttcccaacaa aataacgcca ttcttaccac
    32161 attcaacgaa gtaaacatgc agagcgtgat ggatttacgc gcccgctgga aagatcgttt
    32221 tgccgagaag cacggcgtga agctgggctt tatgtccttc tttgttaagg ccgttacccg
    32281 ggcgctggag cgcttccctg tagtgaatgc cagcgttgat ggcaacgaga ttatctggcg
    32341 tgattattgc gatatcggca ttgcagtgag cagcaaccgc ggcctggtag tgcctgtgtt
    32401 acgcaatgcg caatcactct ctttggtgga aattgaacgg caaattgccg aatacgccac
    32461 acaggcacgc aatggcaaat tgccgctgga ggcgttacag ggcggcacgt tctctatcac
    32521 caacggcggt acttttggtt cgatgatgtc aacgccaatc attaacccgc cgcagtcggc
    32581 cattcttggg atgcatgcca tcacgccgcg cccggtcgca gaaaacggcc aggttgttat
    32641 tcgtcccatg atgtaccttg ctctcagcta tgaccatcgc attatcgatg gtcaggaagc
    32701 cgtgcagaca ttggtggcaa tacgtgagct actggaatca ccggaacaac tgttgctgga
    32761 tctttaagga gggtgccgat gagtacaatt tttgatgtgg cagttatggg gggtgggcca
    32821 ggtggttacg tagcggcact gcgggctgcg cagaatgggc tttccgtagt atgtattgat
    32881 gatggtgtta atgcacaggg cgaaccttcg ccgggcggta cgtgtctcaa tgtcggctgt
    32941 attccgtcaa aatcgttgct gcaatcttcc gaactgtacg cgcaagtgca gcatgaggcg
    33001 agcattcatg gcgttaatgt tgacggcgta tctttcaatg cagctgcgat gattcaacgt
    33061 aaagacgcca tagtaagccg cctgacaatg ggcatcggct tgctgtttaa gaaaaataaa
    33121 gtgaagtacc tgtgcggcct cgcaacgtta gaacatgctc gggatgagat ttggcaatta
    33181 catgtaaacg atcaacaaat ccgcgcccgt aatgtggtga ttgcaacagg ttctcagccg
    33241 cgtcaactgc ctggcgttac cattgataat cagcaaattc tcgataatcg cggcgccctg
    33301 gcgttaaccg ctgtgccgcc gcgtctcggt gtgatcggcg ctggcgtgat tggccttgaa
    33361 ctggggtctg tgtggaatcg ggtcggttca gacgtcacgt tgctggagat ggcgccaacg
    33421 ttcttgcctg cactggaagc tcgcctgtcg aacgaagtcc gtaaagcaat gattgccagt
    33481 gggatgaaaa tgcaactggc agtagagatt gaggctattg aacaacggga tgacggtgtg
    33541 catgtgcgct ggcggcaggg tgagaagcga gaagaaagcc gcttcgataa attaattctt
    33601 gccattggac gtgtaccgcg actttctggt gtcgatctcg tgcaactcgg tcttgaagcc
    33661 gataaccgtg gcggtattgc cgtcgataac ttatgccgta ccgggaaagc cgggctgtgg
    33721 gcaattggtg atgtcgtgcg agggccgatg ctggcgcata aagcgatggc tgaaggtgta
    33781 gtggttgctg atcaaattgc cggactggcg gtagagccga ttaattttgc gctgatccct
    33841 tcggtgattt acacccaacc ggaagttgcg tgggtagggg aaaacgaggc ctcactgaaa
    33901 gccgctggca gagtctttaa caaaggaaat tctctgtttg ccggtaacgg tcgcgcctta
    33961 gcattaggcc aggaaggtgg gcgttgcacg ttatacagtg ataaacacac tgaccgcgta
    34021 cttggcggag cgattgttgg cccgcaggca tcggagctga tcaatgaaat tgcgctggcg
    34081 atgactttta gcgcctctgg cgaggatatt gcctgtgcga tccatgcgca tccgacctta
    34141 agcgaagtca tccacgaagc agcaatggcc ttgaataata aagcattaca cggataacac
    34201 ataccactga gggaatatat gaatttacat gagtatcagg ccaaatcgtt actggctggc
    34261 atggggatgc cgtgtccaaa agagatagcc atccaacaga tttcgcaact ggcagatgcc
    34321 tggcaacata ttgcttgccc gagtaaaggc gcggtactta aagcgcaggt acatgctggt
    34381 gggcgcggta aagctggcgg cgtaaaagtg cttaagcagc tgccggaagc acaggctttt
    34441 gtacagcaaa tgttgggatc gcaactggtg acttatcaga ccgggccaga aggacagtat
    34501 gtcagcagta ttttactgtg cgaaaacatt tatccggtac gccaggaact ctattttggc
    34561 atggtggtgg atcgtgaaag ccagcgggtc acgtttattg tcagcccgga aggaggtgtg
    34621 gaaattgaaa aagtcgccca tgagacaccg gagaaaatca gcagtgtcag cattgatcca
    34681 ctgacaggcg tgcagccttg ccatatccgc gaaatgtttg ctgttctgca actggaacac
    34741 ggactgtttg ctgcctttag tcgcctggtt aatcaggcgt ggaaagcctt taacgaactg
    34801 gattttgccc tgctggaaat taaccctctg gtattgcggg aaacgggcga atttatgtgt
    34861 gcagacgcga aagtatcgct ggatgataac gcgctgtatc gtcacccgga actccaggtg
    34921 ctacgtgatg aaacccagga agatccacgc gaaagccagg cagcgaagct tgatcttaat
    34981 tatgtctcgc tggacggaaa tatcggctgc atggtcaatg gtgcagggct ggctatggca
    35041 accatggaca tcatcaagct atacggtgaa caacccgcaa acttcctgga tgttggcggt
    35101 ggcgcaaccc aggagcgcgt cagtgaagcc tttcggctga ttgtttccga cagcaaagtg
    35161 aaagccattc tggttaatat ttttggtggg attgtccgtt gcgacatgat cgccagagcc
    35221 attattcatg ctctgaatga ggcccgtatc accctgcctg tcgtggtgcg tctctccggt
    35281 aataacgctg ccgaagggca gcggttactg gcagaaagtg gcctgacggt ggaagccgtc
    35341 aattctctgg atgatgccgc taaacgcatc attgcgttac tgaattaaca ggaggttaag
    35401 atgagcgttt tgatcaacaa acacacgcgg gtactggtgc agggcattac cggcaaaaac
    35461 ggcacatttc atacagagca ggctattgcg tatggcacgc agattgtggg tggagtgacg
    35521 ccaggtaagg gcgggcaacg gcatctggat cgcccggttt ttgattcaat gaaagaagcg
    35581 atgcgacaaa ctgaggctga tgccagcgtg atttatgtgc ctgcaccgtt tgtgctggat
    35641 tctgttgttg aggctattga agctggcgtc aaattggttg tggtgatcac tgaaggtgta
    35701 ccgacactgg atatggtgaa agttcgtcgg ctgcttgact gccacccgga tgtgcggctg
    35761 attgggccaa actgcccagg agttatcacg ccgggtgagt gcaaaatcgg gattatgcct
    35821 ggtgaaatcc accgcccagg gcgtattggg attgtttccc gctccggtac attaacctac
    35881 gaagctgtag cgcaaaccac ggcgctggga cttgggcaat ccacctgtat tggtattggc
    35941 ggtgatccgg ttccggggac taactttatt gatgcgctgc gtttattcga aaatgatccg
    36001 caaacagacg cggtaattat gatcggtgaa attggtggta atgcggaaga acgggccgcg
    36061 gaatttattc gccatgaaat gaataaaccg gtcgtaggtt atattgccgg tgtcactgca
    36121 ccaaaaggaa aacgcatggg acacgctgga gccattattt ctggcggtag cggcagtgcg
    36181 gaggataaat tccgggcgtt cgataaagca ggcatggcat ggacacgaaa tccagcatta
    36241 cttggcgaaa cgttatggag tgttattaag taatccataa atgaatatca agggagcata
    36301 tcgctccctt tttttatc
//
LOCUS       DADSZD010000028        35823 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.028, whole genome shotgun sequence.
ACCESSION   DADSZD010000028 DADSZD010000000
VERSION     DADSZD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.028"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            4..1377
                     /locus_tag="J1Z28_001267"
     CDS             4..1377
                     /locus_tag="J1Z28_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7716114.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(1423..2304)
                     /gene="htpX"
                     /locus_tag="J1Z28_001268"
     CDS             complement(1423..2304)
                     /gene="htpX"
                     /locus_tag="J1Z28_001268"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBA7716115.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(2496..4544)
                     /gene="prc"
                     /locus_tag="J1Z28_001269"
     CDS             complement(2496..4544)
                     /gene="prc"
                     /locus_tag="J1Z28_001269"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBA7716116.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLESDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(4564..5262)
                     /gene="proQ"
                     /locus_tag="J1Z28_001270"
     CDS             complement(4564..5262)
                     /gene="proQ"
                     /locus_tag="J1Z28_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBA7716117.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(5359..5856)
                     /locus_tag="J1Z28_001271"
     CDS             complement(5359..5856)
                     /locus_tag="J1Z28_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HBA7716118.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            5986..7269
                     /gene="yebS"
                     /locus_tag="J1Z28_001272"
     CDS             5986..7269
                     /gene="yebS"
                     /locus_tag="J1Z28_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBA7716119.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANI
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNACFDD"
     gene            7238..9871
                     /gene="yebT"
                     /locus_tag="J1Z28_001273"
     CDS             7238..9871
                     /gene="yebT"
                     /locus_tag="J1Z28_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBA7716120.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPSEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            9951..11390
                     /gene="rsmF"
                     /locus_tag="J1Z28_001274"
     CDS             9951..11390
                     /gene="rsmF"
                     /locus_tag="J1Z28_001274"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBA7716121.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGD
                     LFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPHAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            11509..11745
                     /locus_tag="J1Z28_001275"
     CDS             11509..11745
                     /locus_tag="J1Z28_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBA7716122.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            11766..12041
                     /locus_tag="J1Z28_001276"
     CDS             11766..12041
                     /locus_tag="J1Z28_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBA7716123.1"
                     /translation="MAGYLSWLFPRCKISPKLNGTAPHFGDEMFALVLFVCYLDGGCE
                     DIVVDIYNTEQQCLYSMSDQRIRHGGCFPIEDFIDGFWRPAQEYGDF"
     gene            complement(12042..12698)
                     /gene="pphA"
                     /locus_tag="J1Z28_001277"
     CDS             complement(12042..12698)
                     /gene="pphA"
                     /locus_tag="J1Z28_001277"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBA7716124.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(13093..13434)
                     /locus_tag="J1Z28_001278"
     CDS             complement(13093..13434)
                     /locus_tag="J1Z28_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBA7716125.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(13447..14319)
                     /gene="copD"
                     /locus_tag="J1Z28_001279"
     CDS             complement(13447..14319)
                     /gene="copD"
                     /locus_tag="J1Z28_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBA7716126.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(14323..14697)
                     /gene="yobA"
                     /locus_tag="J1Z28_001280"
     CDS             complement(14323..14697)
                     /gene="yobA"
                     /locus_tag="J1Z28_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBA7716127.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            14836..15066
                     /locus_tag="J1Z28_001281"
     CDS             14836..15066
                     /locus_tag="J1Z28_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBA7716128.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            15168..15824
                     /locus_tag="J1Z28_001282"
     CDS             15168..15824
                     /locus_tag="J1Z28_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBA7716129.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLRCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            15848..16510
                     /gene="exoX"
                     /locus_tag="J1Z28_001283"
     CDS             15848..16510
                     /gene="exoX"
                     /locus_tag="J1Z28_001283"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBA7716130.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(16507..18567)
                     /gene="ptrB"
                     /locus_tag="J1Z28_001284"
     CDS             complement(16507..18567)
                     /gene="ptrB"
                     /locus_tag="J1Z28_001284"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBA7716131.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWKTLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQVYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     QSAD"
     gene            complement(18774..19433)
                     /locus_tag="J1Z28_001285"
     CDS             complement(18774..19433)
                     /locus_tag="J1Z28_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBA7716132.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(19760..20116)
                     /gene="yebF"
                     /locus_tag="J1Z28_001286"
     CDS             complement(19760..20116)
                     /gene="yebF"
                     /locus_tag="J1Z28_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBA7716133.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(20183..20473)
                     /gene="yebG"
                     /locus_tag="J1Z28_001287"
     CDS             complement(20183..20473)
                     /gene="yebG"
                     /locus_tag="J1Z28_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBA7716134.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            20607..21785
                     /gene="purT"
                     /locus_tag="J1Z28_001288"
     CDS             20607..21785
                     /gene="purT"
                     /locus_tag="J1Z28_001288"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBA7716135.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(21841..22482)
                     /locus_tag="J1Z28_001289"
     CDS             complement(21841..22482)
                     /locus_tag="J1Z28_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBA7716136.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(22519..24330)
                     /gene="edd"
                     /locus_tag="J1Z28_001290"
     CDS             complement(22519..24330)
                     /gene="edd"
                     /locus_tag="J1Z28_001290"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020244583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBA7716137.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(24565..26040)
                     /gene="zwf"
                     /locus_tag="J1Z28_001291"
     CDS             complement(24565..26040)
                     /gene="zwf"
                     /locus_tag="J1Z28_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBA7716138.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            26378..27247
                     /locus_tag="J1Z28_001292"
     CDS             26378..27247
                     /locus_tag="J1Z28_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7716139.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCINCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            27375..28817
                     /gene="pyk"
                     /locus_tag="J1Z28_001293"
     CDS             27375..28817
                     /gene="pyk"
                     /locus_tag="J1Z28_001293"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBA7716140.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVLPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(28949..29920)
                     /gene="lpxM"
                     /locus_tag="J1Z28_001294"
                     /gene_synonym="msbB"
     CDS             complement(28949..29920)
                     /gene="lpxM"
                     /locus_tag="J1Z28_001294"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBA7716141.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEIRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(30039..31361)
                     /gene="mepM"
                     /locus_tag="J1Z28_001295"
     CDS             complement(30039..31361)
                     /gene="mepM"
                     /locus_tag="J1Z28_001295"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBA7716142.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(31377..32309)
                     /gene="znuA"
                     /locus_tag="J1Z28_001296"
     CDS             complement(31377..32309)
                     /gene="znuA"
                     /locus_tag="J1Z28_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBA7716143.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            32388..33143
                     /gene="znuC"
                     /locus_tag="J1Z28_001297"
     CDS             32388..33143
                     /gene="znuC"
                     /locus_tag="J1Z28_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBA7716144.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            33140..33925
                     /gene="znuB"
                     /locus_tag="J1Z28_001298"
     CDS             33140..33925
                     /gene="znuB"
                     /locus_tag="J1Z28_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBA7716145.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(34070..35080)
                     /gene="ruvB"
                     /locus_tag="J1Z28_001299"
     CDS             complement(34070..35080)
                     /gene="ruvB"
                     /locus_tag="J1Z28_001299"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBA7716146.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(35089..35700)
                     /gene="ruvA"
                     /locus_tag="J1Z28_001300"
     CDS             complement(35089..35700)
                     /gene="ruvA"
                     /locus_tag="J1Z28_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBA7716147.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         8506 a         9277 c         8888 g         9152 t
ORIGIN      
        1 cctatgccaa aagttcaggc cgacggcctg ccattgcccc tgcgatacgg tgcgatatta
       61 accattgtga ttggtatttc gatggctgtc cttgacggcg caatcgccaa cgtcgccttg
      121 ccaacaatcg ctacggacct tcatgccacg ccggccagtt ccatctgggt agtgaacgcc
      181 tatcaaatcg ccattgtcat ctccctgctc tcgttttcgt ttctgggcga tatgtttggc
      241 tatcgacgta tttataaatg cggtctggtc gtttttctgt tgtcttcact gttctgcgcc
      301 ctttctgatt cgctgcaaat gctcaccctt gcgcgtgtca tacaaggttt cggcggtgca
      361 gcgttgatga gcgttaatac cgcacttatc cgcctgatct atccacaacg ttttctgggt
      421 agagggatgg gcataaactc gtttattgtt gccgtctctt ctgctgccgg gccgacaatt
      481 gctgcagcaa tcctctccat cgcatcctgg aaatggttat ttttaatcaa cgtaccgttg
      541 ggtattatcg ccctgcttct ggcgatgcgt tttctgccac ccaatggttc tcgcgccagt
      601 aaaccccgtt tcgacctgcc cagcgccgtg atgaacgcgt taaccttcgg cctgcttatt
      661 actgcattga gtggtttcgc tcaggggcaa tcactgacat tgattggtgc ggaactggtg
      721 gtaatggttg tcgttggtat tttctttatt cgccgccagc tttctcttcc cgtaccgctg
      781 ctaccggtgg atttactgcg tatcccgctg ttttcacttt ctatttgcac atctgtttgc
      841 tctttctgcg cacaaatgct ggcaatggtt tcccttccct tttacctgca aaccgtgctc
      901 gggcgtagtg aagtcgaaac aggtttactt ctgacaccgt ggccgttagc aacgatggtg
      961 atggctccac tggcaggcta tttgattgaa cgcgtacatg caggattgct gggtgcttta
     1021 gggttattca tcatggctgc ggggcttttt tccctggttc tgctgcccgc gtcacctgcg
     1081 gatatcaata ttatctggcc gatgatctta tgtggcgctg gatttggctt gttccagtca
     1141 cccaataacc acaccattat tacctccgct cctcgcgaac gtagcggtgg agccagtggc
     1201 atgttaggga cggctcgtct tctgggtcag agtagcggcg cggctctggt agcgctgatg
     1261 ctaaatcagt ttggtgataa tggtacgcac gtctcgctga tagctgcggc tattctggca
     1321 gtgattgctg cctgtgtcag tggtttacgt atcactcagc cacgatccag ggcataaaaa
     1381 aagcgcgtcg atcaggacgc gctttttagt atttacttca tattacttca ggtattcacc
     1441 cgtacgcaga gcttcaatac gtttatccag cggcgggtga gtcatgaaca actcactgag
     1501 cgatttcgac ttcccgttaa tgcagaaagc catcatgctg gttgcttctt gcggttcata
     1561 gctggttttc aggcgctgca atgcagcaat cattttctcg cgaccaacca gttttgctga
     1621 acccgcatcg gcgtggaatt cacgatgacg cgagaaccac atggtgataa tgctcgccag
     1681 aataccaaac accagttcca gaaccgttgc aaccgcaaag tagatcagcg ggttgccgtt
     1741 gctctcttca ccttcatcac ggtttccgcc cataaaaccc gcggcaagct gcgccagaat
     1801 acgggaaata aagataacga aggtgttcac cacgccctga atcagcgtca tggtgaccat
     1861 atcaccattg gcgatgtggc tgatttcgtg agcaattacc gcctcggctt catccgggct
     1921 catgttctgc agcaaaccgg tgctgacagc aaccagagag gcatcacggc gcgcaccggt
     1981 tgcaaaagca ttgatgtccg gcgcatggta gatagccact tgcggcatag cgatccccgc
     2041 ctgacgagcc tgggttgcta cagtattgac cagccaacgt tccctttcgt tacgcggttg
     2101 ctcgatcact tccccgccaa cagatcgtaa tgccatccat ttggacatca gaagcgaaac
     2161 gaaggaacca ccaaaaccga acagcaaggc catgatcatc agcccctgaa cgctgctcga
     2221 ctgtatccct gtcaggctca gtaccagccc gaaaacgacc attacggcca ggttcgttag
     2281 caggaagagc gcgattcgca tcataatttt ctttttacct cagtttaaca aaacgcaata
     2341 tgcgataccc acatcgtatg ggttacgcga ctattttcaa gtctggatag tgcgtaagtc
     2401 accagaaaga cacaacttta cattttgtag catctgattt acggcatctt gccgctgtta
     2461 aaaaatcagg cacaatttct tgtgcctgat tgatattact tgacgggagc gggttgttcc
     2521 gcgggtctgg ctttttcaag cttcgccaga tcgagtgcga tattcaccgt ctcatccaga
     2581 taaggatctg gctcctggta atctttcggt agatcatcca gtttcttcaa ctccggttta
     2641 ccttcgcgtt taaagcgttc gttcaaacgc gccagacgcg tcgcatcatc ttcattattc
     2701 tctttctcac gcacagcgta attcagagaa acgatattgc gcttgtcctt catagcgttg
     2761 aagcgcgcga tatccttcat gatgttctgg aactcaggat ctttcgcgat acgcgcatta
     2821 tgttccttca gcagctccgg ttcaaaggcc gttaaatctc ctgatttcac ataagtcgcg
     2881 gcatcaatgc tatcccacgg cagcgcgtta tcttcgaatt tctcacccgt ttccgtttct
     2941 tcattacccg tcggcatgat gatgtctggc gttacgcctt tacgttgcgt actgccgccg
     3001 ttaacgcgat agaatttctg gatcgtgtac tgcacagaac ccagcgctgg ccattcagga
     3061 cgtaacatct gatcgtaaat acggttcaat gaacggtact gctgaacggt gcctttacca
     3121 aatgtcggtt cacccacaac cagggcacga ccgtaatcct gcattgccgc ggcaaagatt
     3181 tctgaagccg aagcactgaa gcggtcaacc agcaccacca gcgggccttt atagaagacc
     3241 tgtccgtcgg tgtcgctgtc ttcacgaacc ttgccgttgt tatcgcggac ctgaacaatc
     3301 gggccggatg gaatgaacag accggagagc gatacggctt cagtcaacgc cccaccacca
     3361 ttgctacgca ggtcgataat cacactgctg acattctgtt tttccagttt ctgcagttgc
     3421 actttgacat cgtctgtcaa acccacatag aagcccggta tatccagcac gccgactttc
     3481 tctttaccga cggtcttcac cgacatttta accgcgcggt cttcgagacg aatacgttca
     3541 cgggtcaacg ttacagtacg cgtcttggtc cctttaccag caggtaaaat ttccagacga
     3601 actttactgc ccttaggccc tttaattaag gccaccacat catcaagacg ccagccaatc
     3661 acgtcaacca tcggtttgcc tgtttgacca acaccgacaa ttttgtcacc aacgctgata
     3721 gctttgctct tcgctgccgg acctcctgcc accatcgaat tgataacggt gtagtcatca
     3781 tccatttgca gcactgcgcc aataccttcc agcgacaaac tcatttcagt gttgaactgt
     3841 tcggtattac gcggggaaag atagttggta tgcgggtcga tttcacgcgc aaacgccgtc
     3901 attgccagcg agaaaacatc ttcgctgttg gtttgcgcca gacgacgaat ggcaaatttg
     3961 tagcggcgag tcagggtttc acgaatttcc ttatccgttt ttcctgtcag cttcaggctt
     4021 aactcgtcga atttgacttt actgtcccac agcgcgttca actcagcctc gtttttcggc
     4081 cagggcgctt tgctgcggtc aaggttataa gtgtcgttgc cggtgaaatc catcggcttt
     4141 tccagtaccg acaaagcgta ctggtaacgt tcaaaacggc gcttttgcgc cagattgtag
     4201 agatcgtaga aaacgtcgag tttacctgaa cgcagttcat cgcctaactc agttttcttt
     4261 ttcgcgaact gttcaacatc gctttccagc agcacattgt ggctgtaatc gagcagattc
     4321 aggtagcggt caaagatttt ggccgaaaat gcctgatcga ggtcgaactg gcgataatga
     4381 gaacgggtga agcgcgacgt tacgcgctca ctcaccgtcg catgctgcgt ctcttccttt
     4441 aataccggaa tttgatcagc acgcgtgata tcttctacag cgaaggtctg gcctgctatt
     4501 gcaagcaggc cagctaacgc ggtaagccta aaaaacatgt tcatgcctgg cccggcctcc
     4561 gtttcagaac accaggtgtt ctgcgcgcac aatcaaagac atacccgaat tcagctggac
     4621 gcggacgccg tctttggtga tttctaatac ggtggcatcc atcgcgtttt gacccgcttt
     4681 caccttcagg gcttgtccga cagtcagagc tgaaatgtca gaaaccgggg tgtgctgttc
     4741 ttcgcgaggt gcttttactg tttttggcgc tttctctacc ggcttttgcg aacgaggttt
     4801 acgttcagcg ccttctttgc ggcgtggcgt ggtcggacgt ggcttgcgtt cgcggcgcgg
     4861 tgcgtcttct ttctcaccag cagctgcggc agcttcgcgt tttttcgctt gctgttcagc
     4921 acgctgtgcc tgaacacgcg ctttcgcttc ttcaagctgc ttgcgagcat gctcgacatg
     4981 ttgctcgtcc agctcaccgc atgggttgcc gtcaagatcg acacgcgttg cgccgggttt
     5041 aacaccgtaa agataacgcc agctcgaagt gtagagacgt aaagcggatc gcaattgcgt
     5101 tttgctcagg ttcatttccc cggcaacacg atcgaccaaa tcctgaaaaa taccgatttt
     5161 cagcggacgc gcttcacctt ccgcactgaa acagtgggga aaacgttcgg ccagaaacgc
     5221 gattacttct ttactgctat tcaacttagg ttgattttcc atgaaatttc ctgattacaa
     5281 cggacgtagc caacaagcgc aggcatgaac aggcgtcatt ataatgacgc tatcagtaaa
     5341 tgctacgtta tccgttgatt atcctgcgac gctcgcaaag aattttttgt aatccgtcgt
     5401 tgcaagcact ttttcaagct gtgccacaag ctgacgtaag ccttgctcgt cctcgtctgt
     5461 aaagcgaccg aagacggtac tatcgatgtc gagaacacca ataatctgat ttttcaccac
     5521 cagtggcaga acaatttcag aattactcgc cgcatcacag gcaatatgcc cgtcaaacgc
     5581 gtgaacatcc tcgatacgct gcacttgatt gcgggcaacc gcagtgccgc atacaccgcg
     5641 cccaacgggt atccggacac aggcaatttt gccctgaaat ggtccgagca ccagtgtatc
     5701 gtcctcaagc aaataaaaac ctgcccagtt tacgtcagtg agacgctcat ataacaacgc
     5761 actggtgttc gcaagcgttg ccagaaaact ggtttctccc gccatcagcg cgttaaagtc
     5821 gcgatttaaa tccgcgtaaa attctgcttt gttcattata taatcacttg gttgtcttac
     5881 ctggaactgc cagcctatta aaataagcat taaatgcgtt aatgctcaag atcattccca
     5941 tcatgggtta agattaatgt taattcttat tacatttggc acgtcatggc tcttaacaca
     6001 ccacaaatta cgccgacaaa gaagataaca gtgagggcta tcggcgagga actgccgcgt
     6061 ggtgattacc aacgttgccc gcaatgtgac atgctgttta gcctgcccga gataaattct
     6121 catcaaagtg cctattgtcc gcgctgtcag gcaaaaattc gtgacgggcg cgactggtcg
     6181 ctaacgcgcc tggcggcaat ggccttcacc atgctgttgc ttatgccgtt tgcctggggc
     6241 gaaccgctgt tgcatatctg gctgttaggc attcgtattg acgccaacat tatgcaaggc
     6301 atctggcaaa tgaccaaaca gggcgatgcg ataacggggt cgatggtctt tttctgcgtt
     6361 atcggtgccc ccctcattct ggtgacctcc atagcttatt tatggtttgg taaccgattg
     6421 ggaatgaatt tacgcccggt actgctgatg cttgagcggc tgaaagagtg ggtgatgttg
     6481 gatatctacc tggtcggcat tggcgttgct tctataaagg tacaggatta tgcccatatc
     6541 caggcgggtg tcggcttgtt ctcttttgtg gcgttggtga ttttaacgac ggtgacgttg
     6601 tcacatctca atgtcgaaga actgtgggag cgattttatc cgcagcgccc cgctacgcgt
     6661 agggacgaga aactccgtgt ctgtcttggg tgccatttta ccggctatcc tgatcagcgt
     6721 ggtcgctgcc cgcgttgcca tatcccgcta cgcctgcgtc gccgtcatag tctgcaaaaa
     6781 tgctgggcgg cgctgttagc gtcaatcgtt ttgttgttac ctgccaacct gttgcctatt
     6841 tctatcattt atctgaacgg aggacggcag gaagatacaa ttctttccgg aattatgtcg
     6901 ctggcaagta gcaacattgc cgttgcggga atcgtgttta tcgccagtat tctggtaccg
     6961 tttactaaag tgatcgtcat gttcacttta ctgttgagta ttcattttaa atgccagcaa
     7021 ggtttacgca cacgcattct gttactgcgg atggtgacct ggattggtcg ctggtcgatg
     7081 ctcgacctgt ttgtcatatc tttaaccatg tcgctgatta atcgcgatca gatcctcgct
     7141 tttactatgg gaccggctgc gttttatttc ggcgcagcgg taattttgac tattcttgct
     7201 gtggaatggc tggacagccg cttactttgg gatgcacatg agtcaggaaa cgcctgcttc
     7261 gacgactgaa gcgcagatta aaaataaacg ccgtatctca cctttctggc tgctgccgtt
     7321 catcgcgcta atgattgctg gttggctgat ttgggacagt tatcaggacc ggggcaatac
     7381 tgtcaccatc gactttatgt cggcggatgg tattgttcca ggccgtacgc ctgttcgtta
     7441 tcagggcgtt gaagtcggaa cagtgcagga tatcagcctc agcgacgatc ttcgtaagat
     7501 tgaagtcaag gtcagcatca agtccgatat gaaagatgcg ctgcgcgaag agacacagtt
     7561 ctggttggtg acgccaaagg cctcgttggc aggtgtctcc gggctggacg ccctcgtcgg
     7621 tggtaattat atcggcatga tgccgggtaa aggtaaagag caggatcact ttgtcgcact
     7681 cgacacccaa ccgaaatatc ggctggacaa tggcgatctg atgatccacc tgcaagcccc
     7741 cgatctcggt tcactgagca gtggttcatt ggtctatttc cgcaagatcc cggtgggtaa
     7801 agtctacgac tatgccatca atcccaacaa gcaaggcgtg gtgattgatg tcctgatcga
     7861 gcggcgtttt accgatctgg tgaaaaaagg tagccgtttc tggaacgttt ccggcgttga
     7921 tgccaacgtc agtatcagtg gcgcgaaggt gaaactggaa agtctggcgg cactggttaa
     7981 cggtgcgatt gccttcgatt cacctgaaga gtcgaaacct gccgaagcgg aagatacctt
     8041 tggtctgtat gaagacctgg cccatagcca acgtggcgta ataataaaac tggaactgcc
     8101 aggcggtgcc ggattaaccg ccgactcgac gccgttaatg tatcaggggc tggaagtcgg
     8161 acagctgact aaactggatt taaatcctgg tggtaaagtc actggggaaa tgaccgttga
     8221 tcccagcgtc gtcaccctgc ttcgtgaaaa tacccgcatc gaattacgta atccgaaatt
     8281 atcccttagc gatgctaatc tcagtgccct gctgaccggc aaaaccttcg aactggtgcc
     8341 cggcgatggc gagccacgta aagagttcgt ggttgtgcca agcgaaaaag ccctgctgca
     8401 agagccggat gttctgacac tgaccctgac agcaccagaa agttacggta ttgatgctgg
     8461 tcagccgctc attcttcacg gcgtgcaggt aggccaggtt atcgatcgta aactcaccag
     8521 caaaggcgtc acctttaccg tcgccatcga gcctcagcat cgcgaactgg taaaaggcga
     8581 tagcaaattt gtcgtcaaca gccgcgtcga cgtgaaggtg gggctggatg gcgttgagtt
     8641 tctcggtgcc agcgcctcag aatggatcaa tggcggaata cgtattctgc cgggcgataa
     8701 aggcgagatg aaagccagct atccactgta tgccaatctg gaaaaagcgc tggagaacag
     8761 ccttagcgat ttacccacca caaccgtgag tttgagtgca gagacgctgc cggatgtgca
     8821 ggcaggatcg gtagtgctgt accgtaaatt tgaagttggt gaagttatta ctgtgcgtcc
     8881 gcgagctaac gcgtttgata tcgatctgca tattaaaccg gagtatcgca accttctgac
     8941 cagcaacagc gtgttctggg cagaaggtgg ggcgaaagtt cagctgaatg gtagtggtct
     9001 gactgtacag gcatccccgc tctccagagc attaaagggg gctattagct tcgataacct
     9061 cagcggtgcc agcgccagtc agcgtaaagg cgacaagcgt attctgtatg cttccgaaac
     9121 agcggcccgt gcggttggcg gacaaattac gcttcacgct ttcgatgcgg gaaaactggc
     9181 ggtcgggatg ccaattcgct atctcggtat tgatatcggg caaatccaga cgctggatct
     9241 gattaccgca cgcaatgaag tacaggcaaa ggcggtgctc tatccggaat atgtccagac
     9301 ctttgctcgc ggtggtacgc gcttctcagt ggtcacaccg caaatttcgg cagctggcgt
     9361 tgagcatctt gatactatcc tccagccgta tatcaacgtc gaaccaggcc ggggcaatcc
     9421 tcgccgcgac tttgaattac aagaggccac cattactgat tcgcgttacc tggatggctt
     9481 aagcattatt gttgaagcgc cggaagccgg ttcgttaggt atcggtacac ctgtgctgtt
     9541 ccgtggtctg gaagtaggta cggttaccgg aatgacgctg gggacattgt ccgatcgcgt
     9601 gatgattgcg atgcgcatca gtaaacgcta tcaacacctg gtgcgtaaca attccgtctt
     9661 ctggttggca tcgggttaca gtctggactt tggtctgacg ggcggcgtag tgaaaaccgg
     9721 cacctttaac cagtttatcc gtggcggcat cgccttcgcc acgcctccgg gtacgccact
     9781 ggcaccgaaa gcccaggaag gcaagcactt cctgttgcag gaaagtgaac cgaaagagtg
     9841 gcgtgaatgg ggaactgcgc ttcccaaata atgcccactg ctccggcgtg cctgcgccgg
     9901 agcgtttatg ctaaactgcg cgcctgtttt tttgccagtg gtatatgctc gtggcccaac
     9961 ataccgttta tttcccggac gcctttctga cacaaatgcg cgaagcgatg ccttcgacgc
    10021 tctcttttga tgattttctt gccgcctgtc agcgcccgtt gcgccgcagc attcgcgtta
    10081 atacgctgaa aacctccgtt gctgatttcc tgcaattaac tgctccttat ggctggacgc
    10141 ttacgccaat tccgtggtgt gaagaaggtt tctggattga acgcgacaat gaagatgcat
    10201 tgccgttggg tagtaccgcc gagcatttaa gcggcctgtt ttatattcag gaagccagtt
    10261 caatgttgcc tgttgccgcc ttgtttgctg acggtaatgc accacagcgg gtgatggatg
    10321 tcgctgctgc accaggttcc aaaacgacgc aaattgctgc gcggatgaat aacgaagggg
    10381 caatccttgc caatgagttt tccgccagtc gggtaaaagt gttacatgcc aatatcagcc
    10441 gctgtggcat cagtaatgtt gcgctcacgc attttgatgg ccgcgtgttt ggtgcggcag
    10501 tgccagaaat gttcgatgcc attttgctgg acgctccctg ctccggcgaa ggcgtggtgc
    10561 gtaaagatcc cgatgcgcta aaaaactggt caccagaaag caatcaggaa atcgcagcga
    10621 cccaacggga gctgatcgac agcgcctttc atgcattacg tcctggcggt acgctggttt
    10681 actcgacctg taccttaaac cgggaagaaa acgaagccgt ttgcctgtgg ctgaaagaga
    10741 cttaccacga cgcagtagag tttttaccgc ttggcgatct cttccctggt gcaaataagg
    10801 cgctgaccga agacggcttt ttgcatgttt tcccacaaat ttacgactgc gaaggcttct
    10861 tcgttgctcg tctgcgtaaa actcaggcga ttcccgcctt acccacccca aaatacaaag
    10921 tgggcaattt cccgttcagc ccggtgaaag atcgcgaagc cggacaaatt cgtcaggcgg
    10981 ctgcaagtgt tggcttaaac tgggatgaaa acctgcgact ctggcagcgt gacaaagaac
    11041 tgtggttgtt cccggtgggc attgaagccc tgatcggtaa agtccgattt tctcgcttgg
    11101 ggattaaact tgccgaaacg cacaacaaag gttatcgctg gcagcatgaa gccgttattg
    11161 cccttgcctc ccccgacaat gtgaacgctt ttgaactgac accgcaggaa gcggaggagt
    11221 ggtatcgcgg gcgcgatgta tacccgcatg ccgcgccagt ggcggatgat gtattggtta
    11281 ccttccagca tcagccgatt ggtttagcca aacggattgg ttcacgactg aaaaacagct
    11341 acccgcgtga actggtgcgc gacgggaaac tttttaccgg taacgcctga cagcgcacaa
    11401 aaaaaagcgc actttttgac tggcacattc gactgcctca actaggctga aaaatggtgc
    11461 gatcggactg gtcgtaccac aaccggcagc taaatggaga gcacgaagat gaaaaccagt
    11521 gtgcgcatag gcgcttttga aatcgacgac ggcgaattac acggcgaatc gccgggtgat
    11581 cgaacgttaa ccattccttg taaatcagac cccgatttat gtatgcaact ggatgcctgg
    11641 gatgctgaaa ccagtatccc tgccctgcta aatggcgaac actctgtcct ttaccgtacc
    11701 cgttacgatc aacaatctga tgcctggatt atgcgtcttg cctgatccaa aaagaacccg
    11761 tcggcatggc gggttatttg tcctggttat tcccccgttg taaaatctct cctaaactta
    11821 acggtacggc accacacttc ggggatgaaa tgttcgcgct ggtacttttt gtttgctacc
    11881 tggatggcgg ttgtgaagat attgttgtgg atatctacaa cacggaacag caatgtctct
    11941 attctatgag cgatcaacgg atccgccatg gcggttgttt tccgattgag gattttatag
    12001 atggtttctg gcgacctgca caggagtacg gtgattttta attattgcaa ttgcacaaga
    12061 gtcagttctc ccccaaagac agcaccggta tcaatataat gcaggttgcc aatatccacg
    12121 cgatggcgca aaggtgtatg accaaaccag aaatgatcag cacctgcaat tccctgccct
    12181 ttttggcgtt cacctaatcg cgagcggctc cacaagacct gatgcaaatc gacgtccttt
    12241 tgccattcat agacatcatc tggataatcg gcatgagcaa taacatgttt gccggtgcga
    12301 ctgtgtaatt caagaataaa gggcaaatgc tgacattttt ccagcgccgt tttcgcttgt
    12361 ttctgttgat tatctgccag cgcaataaac cagtcgccgc catttatcaa ccataaagac
    12421 atctgccggg atgccagcgc atccatcgcc atctgttcat gattgcctct taccgcacga
    12481 acccaatgtt gttccagtaa ctgcaggcaa cgtaaacttt gcggcccacg atcgataacg
    12541 tctcccactg agataagtaa atctcgccac ggatcaaaac gacaatgcca taatttgcgg
    12601 cgcaactgct caagacaacc gtgtatatcg cctgaaagcc agatatgtcg ccattgatga
    12661 cccgcaattc tctgataaac gggcgcaggc tgtttcatca atattttcct cccgcgctaa
    12721 agatcacata atcttaacaa gaatgttaaa aaacgctgga ctcagacagt agagtgtgtg
    12781 ttatggttga ctataaagtc agcgaaggga atgcttctgg cttttaacag ataaaaagag
    12841 accgaacacg attcctgtat tcggtccagg gaaatggctc ttgggagaga gccgtgcgct
    12901 aaaagttggc attaatgcag gcttagttgc cttgctcttt aagaatagat gacgacgcca
    12961 ggttttccag tttgcgtgca aaatggtcaa taaaaagcgt ggtggtcatc agcttaaatg
    13021 ttaaaaaccg cccgttctgg tgaaagaact gaggcggttt tttattggaa atcaaaaggc
    13081 tattttaggt aattaacaga gtttttcagc tcgctctata aacggtgcca gactcatttt
    13141 ttcgccggga ttgttaggat catcaatctg aatcaccgaa atgggttggg ctttagtctt
    13201 cccactggca acttcctttt gtgcgatatc gtttaaagga tactgcacga gagtacttgg
    13261 gttaatgaca tacaaagcat tacctggtcg gcaagtcagc atcacctctt cgcgattaaa
    13321 cgcccatttg tctttaccca cttcaaaacg gctgacggta atcacctgcg gtgccgccag
    13381 cgcagctgca gaactggtga gtaacagaaa cgccagaata ctttttttca tcattttcgt
    13441 aatccatcaa aaaggttcga gagtcgcaag caggctgatg atcaccagca ctaccgcacc
    13501 tatcgcccat tcgagtttag tcatccagac aaaccaggga gccgcacgat cttcatcctg
    13561 cctcatgcgc ggtacaagaa cataacgatt agccagcgcg atgaccacca taatcatcac
    13621 caggatggct ttgagcaata agagttggcc ccagtaagtc gtaagcgtgg ggggaaatcc
    13681 ggtaatcaac aatgcattaa gcacgccgct tgccagtacg ccgatcaccg caaaatgccc
    13741 gcaccaggaa aaacgcatca gcgcctgaat agcctggtgt cgccaacggc ctttgatgag
    13801 ttgcatacac cagagcaccg gcagtaatcc cccaaaccac gccgccgcac agatcaggtg
    13861 aattgcgtga ttagtctgat ggatttttgc cgttacccct tcattcagcg tcgcatgccc
    13921 gactcccgcc agcagaataa attgcgcggt agtgagcata aacagcaaac gtggcatatt
    13981 ccgcggttgc ataagggcga cgatcaatgt tacgagggcg agaacaattt gccataacca
    14041 gacgccacca aactgcgttt gcaaaacagc cagccaaatg tttggcgaaa atacatctga
    14101 ccatcccgtt cccatcaacc cgccctgaac ggcgagcatt gcagtggcac tgatcaaact
    14161 ccagacggca gcgtgctgtt gtaaacgcag aaaacgcttc gtcagcaagc gacgaatcgt
    14221 taagggggcc agccaggcac cgtacattgc gaagccaaaa accagcatca gcgaggtgaa
    14281 atggataaat cgcagcgcga tccaggtaaa cgccagcatg atttatttca cactaaaggt
    14341 gtaatgtcct ttcgttttat gcccatccac cgaaacaaca tgccagtcaa cggtgtaggt
    14401 tccgggtttc agcgaatcgg ccagtggaac aatcagttgc ttttggtcct gctcattccg
    14461 cttcgccggt aatgttttaa tattttcgtt ttttggcccc gtgatttttg caccactgaa
    14521 tcctgtttca acgccttccg agaagtttaa agtgattgcc tgcggagctg ctgtcacttg
    14581 tgcatttgcc gcaggatact gatgcgttaa atgcgcatgt gcccagacgg aaggtgtcac
    14641 caacgaagtg gtcaggattg ccagcgcgta gcgaagggag cgtgcagttg aagccatatt
    14701 atctattcct ttttgtaata acttttttac agagcataac cttgtctaat gtctgagtcg
    14761 aggatcatca attccggctt gccatcaagg ctcactctta gtaacttttg cccgcgaatg
    14821 atgaggagat taagaatgct gaagaatctg gctaaactgg atcaaacaga aatggataaa
    14881 gtgaatgtcg atttggcggc ggcaggggtg gcatttaaag aacgctacaa tatgccagtg
    14941 atcgctgaag cggttgaacg tgaacagcct gaacatttgc gcagctggtt tcgcgagcgg
    15001 cttatcgccc accgtttggc ttcggtcaat ctgtcacgtt taccttacga gcccaaactt
    15061 aaataaaact tatacatagt tacactttct tacataacgc ctgctaaatt atgagtattt
    15121 tctaaaccgc ccccataatt tgcagtcatt ttgaaaagga agtcattatg tcgttctgga
    15181 aagttgcagc agcgcaatat gagcctcgta aaacctcact caccgaacag gtagctcatc
    15241 atctggagtt tgtgagggcc gccgccaggc aacaatgtca gctgttagtt tttccatcgc
    15301 tttctttact gaggtgtgat tattcgcgac gtgcccttcc tgccccaccc gatctctcgc
    15361 tattagaccc gctttgttat gccgcaacga cctggcgaat gaccatcatt gccggccttc
    15421 ccgttgaata taacgatcgc tttattcgtg gaattgcggt attcgcaccg tggcgaaaaa
    15481 cgccagggat ctaccatcag agttatggtg catgtctggg ccgtcgttcc agaaccatca
    15541 cggtggtcga tgaacaaccg caaggtatgg atatggaccc gacctgttca ctgtttacga
    15601 cagggcaatg tctgggagaa cccgacctgc tggcgtcggc ccgtcgctta caattttttt
    15661 cacatcagta ctcgattgct gtgctgatgg caaatgcccg tggtaacagt gcactatggg
    15721 atgaacatgg tcgtctcatc gttcgcgccg atcgtggttc attattgtta gtcggtcagc
    15781 gttcatcaca gggttggcaa ggcgatatca ttccattacg ctaggctttt tcggcctgga
    15841 gcatgccatg ttgcgcatta tcgatacaga aacctgcggt ttgcagggag ggatcgttga
    15901 gattgcctct gttgatgtca ttgacggaaa aatcgtcaat cccatgagcc acctggtgcg
    15961 tcccgatcgt cctattagtc cacaggcgat ggcgattcat cgcatcaccg aagccatggt
    16021 cgccgataaa ccgtggattg aagatgtgat cccgcactat tacggtagtg aatggtatgt
    16081 cgcgcataac gccagctttg accgccgcgt actgcctgaa atgcccggtg agtggatttg
    16141 cactatgaaa ctggcccgtc gtttgtggcc agggatcaag tacagcaata tggcgctata
    16201 taaaacacgg aagctcaatg tacagacgcc gccgggcctg catcatcacc gcgcgttgta
    16261 tgactgttat atcaccgccg cgttgcttat cgatattatg aacacctctg gctggacggc
    16321 agaacagatg gccgatatca ccggacgtcc atcgttgatg acgaccttca cctttggcaa
    16381 ataccgtggc aaagcggttt ccgacgttgc cgaacgcgat ccgggctatc tgcgctggtt
    16441 gtttaataac ctcgacagca tgagcccgga gctgcgttta acactgaaac attatctgga
    16501 aaatacttaa tccgctgact gagcgggtaa tgttccctgc gccagcgcga ccagaaaagc
    16561 atattccatc gctacgcctt cgtacgattt aaagcgacca gatttaccgc catgacctga
    16621 gtccatgtcg gtacagagca ataaaagatg gtcatcggtt ttcagctcgc gcaatttagc
    16681 gacccatttt gccggttccc aatattgcac ctgagaatcg tgtaaaccgg tcgttaccag
    16741 taaatgcgga taaacctgtg cagtgacgtt gtcatacggg ctgtagcttt tcatatattc
    16801 gtaatattgc ggatcctgcg gattccccca ctcttcaaat tcgccggtgg taagaggaat
    16861 tgattcatcc agcattgttg ttacaacatc aacaaacggc acctgggcga taacgccgtg
    16921 gaacaattcc gggcgttgat taatcgcaac gcccatcaac ataccccccg cactcccgcc
    16981 catcgcataa cagagcgaag gagagccata gcccagtttt aacaatgcat cgcaggcatc
    17041 aagataatca ttaaacgtat ttttcttctt cagaaattta ccgtcctcgt accattgttg
    17101 ccccagctca ccgccgccac gaacatggac aatggcgtag acaaatccac gatccaacaa
    17161 actcaagcgg ctaaaactga aatcggcatc aatacttgcg ccgtaagaac cgtagccata
    17221 caccagcagc gggttgtgtc ctttgcgaaa atgtttgcga tgatagacca gcgaaaccgg
    17281 aacttcgacg ccatcacggg cgactatcca caggtgttca ctgcggtaat tcgccgcatc
    17341 aaaaccagga acttccgttt gttttaatac acgacgctca ccggtatcca tatccagttc
    17401 aaacaaagta tctggtgtgg tcatggacga ataaccataa cgcaatcgcg cggtttcagg
    17461 ttctggattg taggcaatcc aggtcacata ggccggatca tcaaaggcaa tgccaatgac
    17521 ttcccgggtc ttgcggttaa tttgccgcaa actggttaac ccgcgctgac gctcttcaac
    17581 caccagccag tcggtaaaca gcgtaaaccc ttccagcata atgttatcac gtggcggaat
    17641 taactcttcc cactgttgct catcacgcat acgggtacgg tataagccaa agtttttacc
    17701 gtggcggttg gaacgcagat aaaaccgatg ctggtagtga tcaaggctgt attcgtgatc
    17761 tttgcggcga ggcagaaaaa caaacggctc ggcatcggcc atttccgcgt ccagcaggcg
    17821 aacttcactg gtggtggcgc tggccagatg aatgaccaca taatgcttcg aggtcgtttt
    17881 atgcaggctg acgtaatagg tatcgtcttt ttcttcgtaa atcagtttat cttgcgatgc
    17941 tggcgtaccg atggcgtgac gccagacctg ataaggcagc agcgtcactg gatgcttgcg
    18001 aacatagtag aaagtccagg agtcatttgc ccagacaaag ctgggttcaa cgttatccag
    18061 cagttccggg taccagttgc ctgtttccag attacgaaaa cgaatgccgt actgccgtcg
    18121 ggaaagaaaa tcttctgcca gtgccataat ggtgttatct ggcgtaatcg ccattccgcc
    18181 catcgaataa aactcactat gagccgcgcg cttgttggca tcgagcaatg ttttccactc
    18241 gtcccactct tcactgaacg ccgattgacg ctggtagata gcatattcac agcctggttc
    18301 ataaatatgc cgatagcggt agccattttt tatgtagggc gcagaaactt ctctttgcgg
    18361 aatgcggtcg atgatttcct ttaagatgcg atcctgcaag gcttgttgcg aggccatcac
    18421 ccgatgaccg taactatttt cttgctgcag gtagtccagg acttccggct gagaacgcgt
    18481 atcgtcccgc agccagtagt aattatcgat gcgcgtatcg ccatgaagcg tcatggcgtg
    18541 gggaatgcgg gcggcttttg gtagcatgtt attgttcttt ctggttgaaa catcttataa
    18601 gggtggcaaa actcaccggg gatgcaagcg aaacagggta gtcattgctt agatgatgac
    18661 aggtaatggc gcggatatcg aatgttatgc agacagagaa aatcagcctg ctcaaatggc
    18721 cgtgcggttc tggatagccc gaaatcgtca acttcaggct atccagagcg gaattattcc
    18781 gccaaagtgc gtttttgttg ttcgagatcg cgttcaatgc cttcacgaac gtcctgggga
    18841 attttcagcg catcacccag tgcattcagg taactgcgtt ccataaaatg gtcaatatca
    18901 atagccgcgc agctcaggaa atagatttcc agcgcctctt cttcattgcg gaccccggta
    18961 gccaggcgtt gtggatccag cggttgttcg attgcctgct caatgagtac acgccccttc
    19021 tcttccacac cggcttctcg caattgctgg tcgattgccg cacgttcttt ggcatcaata
    19081 tggccatcac ttttagcggc aaagaccagc gcaaggatca aacgttccgt acgctcatcc
    19141 agcggagtac tttgcgcgcc aaactgcggt tcatcctgat gcgccgcgcg aattttatct
    19201 ttgtatttat tccacagcac cgtacccgct accgctccgc cgccaaccag taacgcgttt
    19261 gtgccatatt tcgtaagaag tttacgtgct gatttatttg cgaccagcag cccagccagt
    19321 ccgcctaatg cgccaggcac taaaagtttg cccaatccct gatccgcaga cgaggaggta
    19381 gaagaacggc tttgcccaag cagggattgc agttgattta accagttagc catatttgct
    19441 cctcaataac catttttagt gcctgtagca tagcggatga ggatgtcaga aaatgtatat
    19501 cgagacaaaa gatgcgcaaa taagcggggc ggtaggccgt tattcaaaga aattcgcgcc
    19561 aggtaaggtg ttcactgtat ataaggtgat aagggtattt taggtgaaag tagtttgacg
    19621 ttttgagtgg aactgtttga tgctgattgc ccggttcgac gctgctgcgt cttctcagga
    19681 ctacgattgt gttgcgttgt agggcggaca aggcactcgc gccgtatccg ccaacggtca
    19741 atcgaattat cagaagccat taacgccgct gatattccgc cattcccgct ttgcagtcca
    19801 cgctgacctg gtaatgaata tcggcacttt taccacgcac ggttagcggt actgaccatt
    19861 tatcatcttt cccctgaatg tcctgcaaac tgacccatgc cacgggatcg gcctgaccga
    19921 caattttttg atcatcagcc cagcgcgcca cgcgattttg ttggtaatca cgttttacgc
    19981 tcgcggcaat tccggcagca tccagacctt cacactttgg gaaagtgacc gacttgctgg
    20041 tttcattatt ggcagcgaaa actgatgcgc aggcagaaac caacaacagc cctaaaaacg
    20101 cccctctttt tttcatgttt ttctccatag cacaatgatt caggagaaag catggtacaa
    20161 attgtcagga gcgcaagttg cttcaggccg cgtgtgaggc atcttcctct tcggattcag
    20221 caccgacgac ctgtggggac ggtaatttgc cggttttcag aatggtgctc agcacatctt
    20281 tttgttctgc cagccatagc gaaagggctt cacgttgctc atcttccatt tgcactggag
    20341 aatttgtcag ccaggtgtca agcagatcgg ccgtatcaag catcttgtca taagcatcgg
    20401 cttccttttt gctggtaaac gacattttct cttcgccctc acgaatgact acgtatttaa
    20461 cttcaaccgc catttgcagc ctctcataat aactgtgatt ttatacagta tatttctttt
    20521 cagttgggaa atcaacatca gcaataaaga cacacgcaaa cgttttcgtt tatactgcgc
    20581 gcggaattaa tcaggggata ttcgttatga cgttattagg cactgcgctg cgtccggcag
    20641 caactcgcgt gatgttatta ggctccggtg aactgggtaa agaagtggca atcgagtgtc
    20701 agcgtctcgg cgtagaggtg attgccgtcg atcgctatgc cgacgcacca gccatgcatg
    20761 tcgcgcatcg ctcccatgtc attaatatgc ttgatggtga tgcattacgc cgtgtggttg
    20821 aactggaaaa accacattat atcgtgccgg agatcgaagc tattgccacc gatatgctga
    20881 tccaacttga agaggaagga ctgaatgttg tcccctgcgc tcgcgcaacg aaattaacga
    20941 tgaatcgcga gggtatccgt cgcctggcgg cagaagagct gcagctgccc acttccactt
    21001 atcgttttgc cgatagcgaa aaccttttcc gcgaagcggt tgccgccatt ggctacccct
    21061 gtattgttaa accggtgatg agttcttccg gtaaagggca gacgtttatt cgttctgcag
    21121 agcaacttgc tcatgcatgg gagtacgctc agcaaggtgg tcgcgccgga gcgggccgcg
    21181 taattgttga aggcgtcgtt aagtttgact tcgaaattac cctgctaacc gtcagcgcgg
    21241 tggatggcgt ccatttctgt gcaccggtag gtcatcgcca ggaagatggc gactatcgtg
    21301 aatcctggca accacagcaa atgagcccgc ttgcccttga acgtgcgcag gagattgccc
    21361 gtaaagtggt gctggcactg ggcggttatg ggctgtttgg tgtcgagctg tttgtctgtg
    21421 gtgatgaggt gattttcagt gaagtctccc ctcgcccgca tgacaccggg atggtgacgc
    21481 taatttctca ggatctctcg gagtttgccc tgcatgtacg tgccttcctc ggacttccgg
    21541 ttggcgggat ccgccagtat ggtcctgctg cttctgccgt tatcctgcca caactgacca
    21601 gtcagaatgt cacgtttgat aatgtgcaga atgccgtagg cgcagatttg cagattcgtt
    21661 tatttggtaa gccggaaatt gatggcagcc gtcgtctggg ggtagcactg gctactgcag
    21721 agagtgttgt tgacgccatt gaacgcgcga agcacgccgc cggacaggta aaagtacagg
    21781 gttaaacccg gcaaaaaaac gctacaaaaa tgcccgatcc tcgatcgggc attttgactt
    21841 ttacagctta gcgccttcta cagcttcacg cgccagctta gtaatgcggt cgtaatcgcc
    21901 cgcttccagc gcatctgccg gaaccagcca ggaaccaccg atgcacagca cgcttttcag
    21961 cgccaggtag tcacggtagt tagccggaga aataccacct gtcgggcaga aacggacctg
    22021 ggagaacgga cccgcgatcg cctgcagggc tttcacaccg ccgttagctt cagccgggaa
    22081 gaatttgaac tctttcaaac cgtagtccat acccagcatc agttcggaaa cagtgctgat
    22141 ccccggaatc agaggaatag tcccttcggt agcagctttc agcagcggct cggtcagacc
    22201 cgggctaatt gcaaactgtg cacccgcttc agtgacttct gccagctgct gtggattcag
    22261 caccgtaccg gcacccacaa tcgcttcagg cacttctttg gcgatagcac ggatagcgtc
    22321 aactgcgcac tcggtacgca gagtcacttc cagaacgcgc accccaccag caaccaacgc
    22381 ttttgccatc ggcaccgcgt gttccagttt ttttaccacg ataaccggta caaccgggcc
    22441 ggtggtcagg attgattctg cacttgtttt ccagtttttc atcagagttt tctctcgcct
    22501 gattacaaat ttgtcgtctt aaaaagtgat acaggttgcg ccctgttcgg caccggacag
    22561 tttttcacgc aaggcgctga ataattcacg tcctgttccc acgcgtgacg cgctcaggtc
    22621 aggaatgtgc ggttcgcgag cagccagttc cgcttcgtct accagcagcg tcagttcgcc
    22681 tgtctgtcca ttcacacgaa tgatgtcccc gtcgcgcact tttgccagca gcccgccatc
    22741 gtaggcctct ggtgttacgt ggatagctga cggcacttta cctgaagcgc cggagagtcg
    22801 tccatcggta actaacgcaa ttttgaaaca ccggtccaat aatacaccaa gtggcggcat
    22861 gagtttatgt aattctggca ttccgttcgc ttttggcccc tgatgacgga caacaacgac
    22921 acaatcgcgg tccagtaaac ccgcttcaaa ggccggcata acgtcatgct ggctttcaaa
    22981 aacaaccgct ggcgcttcaa tcacctggtt ctctaccggc acggcagagg ttttcataac
    23041 cgctcgcccc aggttaccgc ttaacacttt tgtcccacca tgatgagaga aaggttgttc
    23101 gaaggacgcg atcacattgt tgtcgagtga tttttctgcc ccttcccgcc agtccagttc
    23161 accattattc agccatggtt caagggtata acgagacaga ccaaagcctg ccaccgtatt
    23221 gacatcttca tgcagcaggc ctgctttgag cagttcacgt accagaaccg gtacgccacc
    23281 tgccgcctgg aagtggttaa tatcggccgg accgttcggg tagagacgtg ccatcagcgg
    23341 tacaacatca gaaaggtcag agaagtcatc ccagttaatc tgaataccgg ctgcgcgcgc
    23401 catcgccacc aggtgcatgg tgtggttagt ggaaccaccg gtcgccagca gtgcaacgat
    23461 accgttcacc accactttct catcgatcat cttaccgatc ggcatccatt cattaccatt
    23521 accggtcatg cgcgtaacct ggcgcgcagc cgcagcggtc aaagcatcgc gcagcggaga
    23581 atccggatga acaaaagaag agcctggcaa ctgcatcccc ataaactcca ccaccatctg
    23641 gttggtgttg gcagtaccgt agaaagtaca tgttcccggc gcatgataag acgcggcttc
    23701 tgactccagt aatgccatgc ggtccacttt accttcggca taaagctggc gaatacgcac
    23761 tttttcttta tttggcaaac cgcttgccat cggtccagac ggcacaaaca ccgcaggcaa
    23821 atgaccaaac gacagggctg ccatggtcag acctgggaca atcttgtcgc acacaccgag
    23881 aaacagagca ccatcaaaca tgttatggga cagccccacc gccgcagaca tcgcaatcac
    23941 ttcgcggctt aacagcgaca attccattcc atcctgcccc tgggtgacac catcacacat
    24001 cgccggaaca ccgcccgcaa cctgaccaac cgcattcgct tcatgcaggg ctttacgaat
    24061 gatttccgga tagtgttcat aaggctggtg agcggagagc atgtcgttat aggaggtgat
    24121 gatggcgata ttgttacgca acatgctttt caaagaggct ttgtcttctg gctggcaggc
    24181 agcgaaaccg tgtgccaggt taccgcatgc caactgcgaa cgatgaacgg tcgaagtttt
    24241 cgcttgttct atccgggcga ggtaagcaga gcgagtctcg cgcgaacgtt caatgattcg
    24301 atttgttacg cgtaacaatt gtggattcat aaaggctcct gaaattgagt tgtcagagca
    24361 ggatgattca caacacgttt cattcagagg attcatgact gaaacgcctg taaccggagc
    24421 tcatagggca aaaacgtttc agtcagtgta ataaaaaaag cctcgtgggt gaatccgcac
    24481 gaggcctgaa agtgtaaaaa ttgttctaca atctgtgcaa gatcatgtta ccggtaaaat
    24541 aaccataaag gataagccca gatattactc aaactcattc caggaacgac cgtcacgggt
    24601 aatcatcgcc accgaggcaa cggggcccca ggttccggcc tgatacggtt tcggcgcatc
    24661 attgtccatc gcccacgcct cagtaatgga gtctacccat ttccaggctt cttccacttc
    24721 atcacgacgt acaaacagtg cctgaatacc acgcatggtt tccagcagca gacgttcata
    24781 ggcatctgcc agatgcgttt gattaaaagt ttctgaatag ctcagatcca gcttggtgat
    24841 ttgcaggtta tgtttgtggt caaggccagg aactttattc agtacctgga tatccacgcc
    24901 ttcatcaggt tgcagacgga tagtcagttt attctgcggc agatcctgcc acgactcttt
    24961 aaacagattc agttcaggtg ttttgaaata gaccacgact tcagaacatt tagtcggcag
    25021 acgtttacca gtacgcaggt agaatggcac accggcccag cgccagttat caatgtcgac
    25081 gcggatcgcc acgaaggttt ctgtattgct gctcttgttc gcgccctctt cttccagata
    25141 gcccggcact tttttgccct gagcgaagcc cgcagtatat tgcccgcgta cggttttttc
    25201 gcgcacattg gagcggtcga tgcggcgcag agacttcagt actttcactt tttcatcgcg
    25261 gatgctgtct gcgctcaggt cagacggcgg agacatcgca atcatgcaaa gaatttgcag
    25321 caggtggttc tggatcatgt cgcgcatctg accggcttta tcaaaatagc cccagcgccc
    25381 ttcgatcccc acttcttctg ccacggtaat ctcaacatga tcaatggtgc gattgtccca
    25441 gttgttcaca aacagagagt tagcaaaacg cagcgccaac aggttcagca ctgtttcttt
    25501 accaagatag tggtcgatac ggtaaacctg gcactcctcg aagtattcgc caacctgatc
    25561 gttgatttcc tgcgaggtcg ccagcgacgt ccccagcggt ttttccatga ctacgcgtgc
    25621 cggtttagca ttcagttttg cctcaccaag ccctttgcaa attgcgccaa aagtgctggg
    25681 cggcatggca aagtagttaa tggtgatacg atttttttga tccagcatcg cgccgagacg
    25741 gctgaatgca gcagtgtcat tgacatcaag attacaaaaa tccagacgtg cactcagggt
    25801 gtcccataaa ccttcatcaa tggtttcttt catgaaagtt tcgagcgcct cgcggacaac
    25861 tttggtataa gccgctttat cccagtcagc acgccctacg ccgataatcc gggtgtccgg
    25921 gttgagctga ccggcttttt ccagttgata cagggaaggc agcaatttac gacgcgcaag
    25981 gtcgcctttc gcgccgaaaa tgaccaggtc acaggcctgg gctgtttgcg ttaccgccat
    26041 gtcattctcc ttaagttaac taacccggta cttaagccag ggtatacttg taattttctt
    26101 acggtgcact gtactgcttt tacgagcttg cgaaaactgt aaacgcttat ccacccgtgc
    26161 gattacggta aaagcgcgca aagtgcggca aaactgataa aaaaatcatc gttttccatt
    26221 gggtaaaaat ctgacactga tcatgttatg aaaaaaaata acaacttttt tatctgcttt
    26281 tgtcattaac acagcacaca ggcgtaatat ctgacaaaac tgcatttcga tttctttcag
    26341 agcggaaatc gtcattaccc gtgagtctct ttacatcatg aatatgctgg aaaaaatcca
    26401 gtctcagctg gaacatttga gcaaatctga gcgcaaagtt gccgaggtca ttctggcttc
    26461 gcccgataac gcgatccatt cgagtattgc tgctctggcg ctggaagcca atgttagcga
    26521 accgacggtg aatcgtttct gtcgcagcat ggacacacgc ggttttcctg attttaaact
    26581 tcatctggca caaagtctgg cgaatggcac tccctatgtt aatcgcaacg tcaatgaaga
    26641 tgacagcgtt gaatcataca cggggaaaat atttgagtcc gcaatggcaa cgctcgatca
    26701 tgtccgtcat tcactggata aatctgccat caaccgcgcc gtcgacttgc tcactcaggc
    26761 aaaaaaaatc gcctttttcg gattaggttc ttcagccgcc gttgcccacg atgcgatgaa
    26821 taagttcttt cgttttaatg ttccggtggt gtactccgat gatatcgtgc tgcaacgcat
    26881 gagttgtata aattgtagcg acggagacgt ggtggtgctg atttctcata ctggaagaac
    26941 aaaaaatctg gtcgagctgg cgcagctggc acgcgaaaac gacgctatgg taattgccct
    27001 cacctctgcg ggtaccccac tcgcccggga agcaacgctg gcaattaccc tcgacgtacc
    27061 ggaagatact gacatttata tgcccatggt ttctcgactt gcacagctga ccgtgataga
    27121 tgtgctggcg acaggattta ctttgcggcg cggtacaaaa ttcagagata acttgaagcg
    27181 ggtcaaggaa gcgctgaagg aatcgcgttt tgataagcag ttacttaatt taagtgacga
    27241 tcgctaaaaa cgactgtcac tgtcctaatc ttatacgaca tccgaatgag attaatttat
    27301 cgccatcgcg gcgttatttc attcggattt catgttcaag caacacctgg ttgtttcagt
    27361 caacggagta ttacatgtcc agaaggcttc gcagaactaa aatcgttacc acgttaggcc
    27421 cggcaacaga tcgcgataat aaccttgaaa aagttatcgc ggcgggtgcc aacgttgtac
    27481 gtatgaattt ttctcacggc tcgcctgaag atcacaaaat gcgcgcggat aaagttcgtg
    27541 agattgccgc aaaactgggg cgtcatgtgg ctattctggg tgacctccag gggcccaaaa
    27601 tccgtgtatc cacctttaaa gaaggcaaag ttttcctcaa tattggggat aaattcctgc
    27661 tcgacgccaa cctgggtaaa ggtgaaggcg acaaagaaaa agtcggtatc gactacaaag
    27721 gcctgcctgc tgacgttttg cctggtgaca tcctgctgct ggacgatggt cgcgtccagt
    27781 taaaagtact ggaagtccag ggcatgaaag tgttcaccga agtgaccgtc ggtggtcccc
    27841 tctccaacaa taaaggtatc aacaaacttg gcggcggttt gtcggctgaa gcgctgaccg
    27901 aaaaagacaa agcagacatt aagactgcgg cgttgattgg cgtagattac ctggctgtct
    27961 ccttcccacg ctgcggcgaa gatctgaact atgcccgtcg tctggcacgc gatgcaggat
    28021 gtgatgcgaa aattgttgcc aaggttgaac gtgcggaagc cgtttgcagc caggaggcaa
    28081 tggatgacat catcctcgcc tctgacgtgg taatggttgc tcgcggcgac ctcggtgtgg
    28141 aaattggcga tccggaactg gtcggcattc agaaagcgtt gatccgtcgt gcacgtcagc
    28201 taaaccgcgc ggtaatcacg gcgacccaga tgatggaatc aatgattact aacccgatgc
    28261 cgacgcgtgc agaagtcatg gacgtagcaa acgccgttct ggatggtact gacgctgtga
    28321 tgctgtcggc agaaactgct gctggtcaat acccgtcaga aactgttgca gccatggcgc
    28381 gcgtttgcct gggggcggaa aaaatcccga gcatcaacgt ttctaaacac cgtctggacg
    28441 ttcagttcga caatgtggaa gaagccattg ccatgtcagc aatgtacgcg gctaaccacc
    28501 tgaaaggcgt tacggcgatc atcaccatga ccgaatcggg tcgtaccgcg ctgatgacct
    28561 cccgtatcag ctctggtctg ccaattttcg ccatgtcacg acatgaacgt acgctgaacc
    28621 tgactgctct ctatcgcggc gttacgccgg tgcactttga tagcgctaat gacggcgtag
    28681 cagctgccag cgaagcggtt aatctgctgc gtgataaagg ttacttgatg tctggtgacc
    28741 tggtgattgt cacccagggc gacgtgatga gtaccgtggg ttctactaat accacgcgta
    28801 ttttaacggt agagtaagta cgttgccgga tgcggcgaaa acgcctcatc cggcctacag
    28861 ttcaatgata attcaacaga tttcgaatct tctgaagcaa acttgaactt atcatcagtc
    28921 gaaggcctct cctcgcgaga ggcatttttt atttgatggg ataaagatct ttgcgcttat
    28981 acggttggat ttcgcccggt ttgcgagttt tcagcaattt taatatccag gtgtattgtt
    29041 ctggtcgcgg accaacaaaa atctcgactt cttcattcat ccgccgcgca atcgtatgat
    29101 catccgcctc taacagatca tccatcggtg ggcgcacctg aatcgtcaga cgatgcgtct
    29161 tgccatcata aatcggaaat agcggtacaa cgcgcgcacg gcacactttc atcaaacgac
    29221 caatcgcggg caacgtcgct ttataggtgg caaagaaatc aacaaattcg ctgtgttctg
    29281 ggccatgatc ctgatcgggt aaataatatc cccagtaacc ctgacgtacc gactggatga
    29341 atggtttaat accatcattt ctcgcatgca gacgaccacc aaagcgacgg cgcaccgtgt
    29401 tccagacata atcaaaaacc gggttgccct gattatggaa catcgctgcc attttctgcc
    29461 cttgcgaggc catcagcatg gcaggaatat cgacggccca accgtgcggc accagaaaaa
    29521 tcactttctc gttattacgt cgtatctctt cgatgatctc cagcccttgc cagtcaacgc
    29581 gcggctgaat tttctccggc ccgcgtattg ccaactcagc catcattacc atcgcttgcg
    29641 gcgcggtggc aaacatctca tctacaatcg cttcgcgttc agcttcacta cgttctggaa
    29701 agcagagcga cagattgatt aacgcacgac ggcgtgagct ttttcccagt cgtccggcaa
    29761 aacgtcccag ccgtgccaga atgggatcac ggaactttgg cggcgttaaa gcgatacccg
    29821 ccatcgctgc tacgcccagc catgctcccc agtagcgcgg gtggcgaaag gatttatcaa
    29881 actcaggaat gtattcgcta ttattttttt tcgtttccat gcttttccag tttcggataa
    29941 ggcaaaaatc aatctggtga tagtgtagcg gcgcaacttg ccccgcacca aataaaaaag
    30001 ccggtactga ctgcgtaccg gctgcgaatg gatgttaatt aatcaaaccg tagctgcggc
    30061 acaatctctt tggcctgtgc caggaattcg cgacgatcgg agccggtcag cccttcggta
    30121 cgcggcagtt ttgccgtcag cgggtttacg gcctgctggt ttatccatac ttcatagtgc
    30181 agatgcggcc cggttgaacg tccggtatta ccggaaagcg cgatacggtc gccacgtttc
    30241 accttctgtc ccggtttcac caggatcttg cgcaagtgca tataacgcgt ggtgtagctg
    30301 cgaccatgac gaatagccac ataataacct gctgcgccac tacgtttggc aaccaccact
    30361 tcaccgtcac ccactgaaag cactggcgta ccttgtggca tggcaaaatc aacacctctg
    30421 tgtggcgcaa cgcgaccggt caccggatta gtacgacgcg ggttaaagtt agatgagata
    30481 cggaactgtt tcgccgtcgg gaatcgcaag aatcctttcg ccagaccagt accgttacga
    30541 tcatagaatt tgccatcttc agcgcggatt gcgtaataat ctttaccttc tgaacgcaaa
    30601 cgtacgccca gcagctggct ttgctcacgt ttaccatcaa gcatttctcg tgacattaac
    30661 accgcaaatt catcgccttt tttcagtttg cggaaatcca tttgccactg catggcttta
    30721 atcactgcgc tcacttcggc gctggttaaa ccggcgtttc tggcgctggc aacaaagctt
    30781 cccccgacgg tacctttcag cagattgttg acccactctc cttgctgcat ttcgctggtc
    30841 attttaaaac cgttagcggc agtacggtca taggttcggg tttcacgacg agacacttcc
    30901 caggtgaggc gctgcagttc gccgtccgcg gttaatgtcc aggagagttg ttgaccgatt
    30961 ttcaggttac gcaattcttt gtcggcagca gccagttggg tgatatcacc catatcaata
    31021 ccatactgat tgagaatgct gcttagcgta tcgccagtgg aaacaacata ttcatgcacg
    31081 cccgcttcac cggcgatttt gtcatccagt tcgtcctggg gaatggcttc atcttcttgt
    31141 gcagcttgat caatcggctc actggcttca ggtaagagcg aacgaatttc gttctgttcc
    31201 agctcaatgg ttttgacaat tggcgtggca tcacggtgat aaacataggg ccgccagaca
    31261 gcgacggcca gagtaagaac ggtgagcgac cccaacataa cgcggtgtgg tcgcggtaaa
    31321 ttattaaacg ccagggcgac agagcgggct atctgttgca cgtaatcact tcctcattaa
    31381 tctcctttca ggcagctcgc atactggttg gctaattgat tcaggaattc tgaatagctt
    31441 gttttaccca gtttgatatt cgtccccagg ggatccaacg ttcccatacg aacggatgtc
    31501 cctcgtgcga cgctctcaac gaccgctggc ctgaactgtg gctcagcaaa aacgcaggtt
    31561 gctttttgct caaccaactg tgttcttatt tcatgtaaac gctgcgcgcc aggttgaatc
    31621 tcagggttaa cggtaaaatg accaagcggt gtcagtccga actgtttttc gaagtagccg
    31681 taagcatcgt gaaaaacgaa ataacctttc cccttgagcg gcgcgagctc gttaccaacc
    31741 tgcgtttcgg ttgaggctaa ttgtgcctca aaatccttca ggttggcgtc aagtttggct
    31801 cgactttgcg gcataagttc cactaatttt ccatggattg caaccgctgt agcccgcgct
    31861 atctctgggg aaagccaaag atgcatgttg aaatcgccgt gatggtgatc ttcgtcactt
    31921 ttttccgcgt gatcgtgatc atcatcatcg tcgtgaatac ttttcatcag cagcggtttc
    31981 acatcttcta gctgcgcaat cgttacctgt ttcgcttctg gtaatttact taccggtttt
    32041 tgcatgaacg cttccatctc cgggccaacc caaacgacta agtccgcgtt ctgtaagcgt
    32101 tttacatccg atggacgcag tgaataatca tgttctgaag cgccgtcagg cagtaaaacc
    32161 tgcgtttctg ttaccccatc agcaatggca gaagcgatga acccaacggg tttaagcgaa
    32221 gcgacaacgg cggcatctgc ggcctgtgtt gcaccgcccc agagagcggc ggataatgct
    32281 gcgaaaagaa gcgttttttt atgtaacata atgcgaccaa tcatcgtaat gaatatgaga
    32341 agtgtgatat tataacattt catgactact gcaagactaa aattaacatg acaagtctgg
    32401 tttccctgga aaatgtctcg gtttcttttg gccaacgccg cgtcctctct gatgtgtcgc
    32461 tggaacttaa acctggaaaa attttgactt tacttgggcc aaacggcgca ggtaagtcga
    32521 cactggtacg ggtagtgctc ggactggtaa cacccgatga aggggttatc aagcgcaacg
    32581 gaaaactgcg catcggctat gtaccgcaga agctgtatct cgacaccacg ttgccactga
    32641 ccgtaaaccg ttttttacgc ttacgccctg gcacacataa agaagatatt ttgcctgcac
    32701 tgaaacgtgt ccaggccggg catctgatta acgcaccgat gcaaaagctc tcgggtggcg
    32761 aaacgcagcg tgtactgtta gcgcgagcat tgttaaatcg accgcaatta ttagtgctgg
    32821 atgaacccac tcagggcgtg gatgtgaatg gtcaggtggc gttatatgac cttattgacc
    32881 aactgcgtcg tgaactggat tgtggcgttt taatggtatc tcacgatctg catctggtaa
    32941 tggcaaaaac cgatgaagtg ctttgcctga atcaccacat ttgttgttcc ggcacaccgg
    33001 aagttgtttc cctgcatccg gagtttattt ctatgtttgg tcctcgtggt gctgaacaac
    33061 tgggtatcta tcgccatcat cataatcatc gtcacgattt acagggacga attgttttgc
    33121 gtcggggaaa tgatcgctca tgattgaatt attatttccc ggttggttag ccgggatcat
    33181 gctcgcctgt gccgcgggtc cgctgggttc gtttgtagtc tggcgtcgta tgtcttattt
    33241 cggtgatacg ctggctcatg cctcattact tggcgtcgcg tttggtttgt tgctggacgt
    33301 gaatccattc tatgcggtga ttgcagttac gctgctgctg gcgggcggtc tggtatggct
    33361 ggagaagcgt ccacagctgg cgatcgacac gttattaggg attatggcgc acagtgccct
    33421 gtcgctgggc ctggtggtcg ttagtctgat gtctaatatt cgtgttgatt tgatggctta
    33481 cctgttcggt gatttactgg cagtgacgcc agaagatctc atctctattg cgattggcgt
    33541 ggtcatcgtg gtggctattt tgttctggca atggcgcaat ttgctgtcga tgacgattag
    33601 cccggatctg gcgtttgttg atggtgtgaa attacagcgc gtgaaattgt tgttgatgct
    33661 ggtgacggca ttgacgattg gtgtagcgat gaaattcgtc ggcgcgttga ttattacttc
    33721 actgctgatt attcctgctg ctactgcacg tcgctttgcc cgcacgccgg aacagatggc
    33781 tggtgtcgct gttttggtgg ggatggtggc agtgactggc ggtttaacct tttccgcatt
    33841 ttacgataca cctgcaggcc cgtcggtggt gctatgcgcg gcactgttat ttattatcag
    33901 tatgatgaaa aaacaggcca gctaatcttt cgctgaacat atttgtcgga tgcggcgcga
    33961 gcgccttatc cgacctacgg ttcgctatct ctggtaggcc tgataagacg aacagcgtcg
    34021 catcaggcac actgccagtg tcggatgcgg cacgagcgcc caatccgact tacggcattt
    34081 ctggcggcgt tatgccaaag tgattccacg cccgcgtcgt cgccatacgc ccacgcggtg
    34141 tacgctgcaa aaagccttgc tgaatcaaat aaggttccag tacatcctca atggtttcac
    34201 gttcttcgcc aatggctgcc gccaggttat ccagacctac cggcccacca aagaacttat
    34261 cgattaccgc cagcaacaat ttgcggtcca tataatcgaa accttcagca tcgacattca
    34321 acatatccag cgcctgagca gcgatatctg ccgagatggt gccatcgtgc ttcacttcgg
    34381 cgaaatcacg cactcgacgc agcagacggt tggcaatacg tggcgtaccg cgcgcacgac
    34441 gagcaacttc cagcgcgccg tcatcactca tctcaagccc cataaagcgt gcgctgcgac
    34501 tgacgatata ttgcagatcc ggcacctgat aaaactccag acgttgcaca ataccaaaac
    34561 gatcgcgcaa cggtgatgtc agcgaacctg cgcgcgtggt tgcaccaatc agggtaaacg
    34621 gcggcaaatc aattttaatg gagcgtgccg ccggaccttc accaatcatg atatccagtt
    34681 ggtaatcttc cattgccgga tacaacacct cttccaccac tggtgaaaga cggtggatct
    34741 catcaataaa cagtacatcg tgtggttcaa ggttagtgag cattgctgcc agatcgcccg
    34801 ccttttccag caccggacca gaagtcgtgc gtaaattaac gcccatttca ttggcgacaa
    34861 tattggcaag cgtagtttta cccaaccccg gaggaccaaa aatcaacaga tgatcgaggg
    34921 catcgccgcg cagtttcgct gctttgatga aaatctccat ctgcgaacga acctgcggct
    34981 gaccaacata ctcttccagt aatttagggc ggatggcgcg atctgccaca tcttccggca
    35041 aagtggtacc ggcagaaatc agacggtctg cttcaatcat cctttacctc ataacgcggc
    35101 gcgtagggct tcgcgaatta atgtttcact gctggcgtca gggcgagcga ttttgctcac
    35161 catgcggctt gcttcttgtg gtttatagcc cagtgccacc agcgcagcaa ccgcttcctg
    35221 ttcagcatcg tcggtcgccg ggctggcagg agacgtgagt accaggtcgg cggctggcgt
    35281 aaagagatcg ccatgcaaac ctttaaatcg gtctttcatt tcgacaatca agcgttcggc
    35341 ggtttttttg ccaatacccg gcagtttcac cagtgccccc acttcttcac gctcaacggc
    35401 attaacgaac tgctgcgctg acattccgga gaggatcgcc agcgccaact tcgggccgac
    35461 gccgttggtt ttgatcaact ctttgaacaa cgtgcgctct tgtttattgt taaaaccgta
    35521 cagcagttgc gcgtcttcac gcaccacaaa gtgggtgaaa acgatcgctt cctgacccgc
    35581 ttcagggagt tcataaaaac aggtcatcgg catatgcact tcatagccta cgccgcccac
    35641 ttcaattaac accagcgggg gttgtttttc aatgatgatg cctctgagtc tgcctatcac
    35701 atgacgctcc tgcgtaatga atcaaagata atgctgtatg ataaaaaaat gctggataga
    35761 tatccagcga aggatgaaga aaacttgcga ggtgtctcga tgatctgaaa aatggcgcag
    35821 tat
//
LOCUS       DADSZD010000029        34579 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.029, whole genome shotgun sequence.
ACCESSION   DADSZD010000029 DADSZD010000000
VERSION     DADSZD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.029"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(15..1268)
                     /locus_tag="J1Z28_001301"
     CDS             complement(15..1268)
                     /locus_tag="J1Z28_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HBA7716148.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1500..2831
                     /gene="argA"
                     /locus_tag="J1Z28_001302"
     CDS             1500..2831
                     /gene="argA"
                     /locus_tag="J1Z28_001302"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBA7716149.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEKKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2913..4739)
                     /gene="recD"
                     /locus_tag="J1Z28_001303"
     CDS             complement(2913..4739)
                     /gene="recD"
                     /locus_tag="J1Z28_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBA7716150.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(4739..8281)
                     /gene="recB"
                     /locus_tag="J1Z28_001304"
     CDS             complement(4739..8281)
                     /gene="recB"
                     /locus_tag="J1Z28_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBA7716151.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYKRLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHVSELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEL
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(8274..11162)
                     /gene="ptrA"
                     /locus_tag="J1Z28_001305"
     CDS             complement(8274..11162)
                     /gene="ptrA"
                     /locus_tag="J1Z28_001305"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBA7716152.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAIFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFYSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11338..14706)
                     /gene="recC"
                     /locus_tag="J1Z28_001306"
     CDS             complement(11338..14706)
                     /gene="recC"
                     /locus_tag="J1Z28_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBA7716153.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELALRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14719..15042)
                     /locus_tag="J1Z28_001307"
     CDS             complement(14719..15042)
                     /locus_tag="J1Z28_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBA7716154.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLHAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15027..15434)
                     /locus_tag="J1Z28_001308"
     CDS             complement(15027..15434)
                     /locus_tag="J1Z28_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBA7716155.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTLPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIFFSPRGWSDFCPLKERALCQLP"
     gene            complement(15431..15994)
                     /locus_tag="J1Z28_001309"
     CDS             complement(15431..15994)
                     /locus_tag="J1Z28_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBA7716156.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(15985..16455)
                     /locus_tag="J1Z28_001310"
     CDS             complement(15985..16455)
                     /locus_tag="J1Z28_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBA7716157.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(16640..17434)
                     /gene="thyA"
                     /locus_tag="J1Z28_001311"
     CDS             complement(16640..17434)
                     /gene="thyA"
                     /locus_tag="J1Z28_001311"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBA7716158.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17441..18316)
                     /gene="lgt"
                     /locus_tag="J1Z28_001312"
     CDS             complement(17441..18316)
                     /gene="lgt"
                     /locus_tag="J1Z28_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBA7716159.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18467..20713)
                     /gene="ptsP"
                     /locus_tag="J1Z28_001313"
     CDS             complement(18467..20713)
                     /gene="ptsP"
                     /locus_tag="J1Z28_001313"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7716160.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20726..21256)
                     /gene="rppH"
                     /locus_tag="J1Z28_001314"
     CDS             complement(20726..21256)
                     /gene="rppH"
                     /locus_tag="J1Z28_001314"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBA7716161.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            21941..22630
                     /gene="mutH"
                     /locus_tag="J1Z28_001315"
     CDS             21941..22630
                     /gene="mutH"
                     /locus_tag="J1Z28_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBA7716162.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22699..23412
                     /locus_tag="J1Z28_001316"
     CDS             22699..23412
                     /locus_tag="J1Z28_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA7716163.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23550..23768
                     /gene="ygdR"
                     /locus_tag="J1Z28_001317"
     CDS             23550..23768
                     /gene="ygdR"
                     /locus_tag="J1Z28_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBA7716164.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23876..24916
                     /locus_tag="J1Z28_001318"
     CDS             23876..24916
                     /locus_tag="J1Z28_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBA7716165.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24948..26141)
                     /gene="lplT"
                     /locus_tag="J1Z28_001319"
     CDS             complement(24948..26141)
                     /gene="lplT"
                     /locus_tag="J1Z28_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBA7716166.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAACALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26134..28293)
                     /gene="aas"
                     /locus_tag="J1Z28_001320"
     CDS             complement(26134..28293)
                     /gene="aas"
                     /locus_tag="J1Z28_001320"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBA7716167.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERDWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELMRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28879..29910
                     /gene="galR"
                     /locus_tag="J1Z28_001321"
     CDS             28879..29910
                     /gene="galR"
                     /locus_tag="J1Z28_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBA7716168.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29917..31179)
                     /gene="lysA"
                     /locus_tag="J1Z28_001322"
     CDS             complement(29917..31179)
                     /gene="lysA"
                     /locus_tag="J1Z28_001322"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBA7716169.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31301..32236
                     /gene="lysR"
                     /locus_tag="J1Z28_001323"
     CDS             31301..32236
                     /gene="lysR"
                     /locus_tag="J1Z28_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBA7716170.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHGENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32223..32915)
                     /gene="ygeA"
                     /locus_tag="J1Z28_001324"
     CDS             complement(32223..32915)
                     /gene="ygeA"
                     /locus_tag="J1Z28_001324"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBA7716171.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGCAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33044..34462)
                     /locus_tag="J1Z28_001325"
     CDS             complement(33044..34462)
                     /locus_tag="J1Z28_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBA7716172.1"
                     /translation="MVTINTESALTPRPLRDMRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVMAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT         8513 a         9486 c         8528 g         8052 t
ORIGIN      
        1 aataaataaa aacattatcc ccttctcgcc agcgtcgccc catcggcaaa atacgcttta
       61 attcccgcaa gaatagactc cgcaacttcc tgctggaaag tcgccgtttt cagtttacgc
      121 tcttcctcaa cgttactgat aaacgccgtt tcgaccagaa tggagggaat atctggtgcc
      181 tttagtacgg caaacccagc ctgttcaact tgatttttat gcagcttgtt gattttaccg
      241 agcttattca gcaccgcttt accaaactta aggctgtcgg caatggtcag cgactgcacc
      301 atatcgaaca tggtgtggtc gacatagcgg tcaccgcttt tgcttacgcc accaatcaag
      361 tccgaggcgt tctgggtttg tgccagatat tttgccgcag tactggttgc gcctttggtt
      421 gagagcgcaa acaccgagga accgctcggc tggcgactgg taaaggcgtc ggcatggata
      481 gaaacaaaca agtcagcgcg ctgtttctgg gcttttgcta cgcgcacttg caacggaatg
      541 aagatgtctt cattgcgcgt catgtacacc ttcatattgc cctctttctc gatcagagag
      601 cgcagacggc gagctatttg caataccacg tctttttcgc gcgttttgta tttccccacc
      661 gcaccggagt cttcgccacc gtggccaggg tcaagcataa tgacaatcgg acgatcccgc
      721 ccagctttac ccggttgtgg accactttgt gctggcggca cctgcttttc gaggtcgcct
      781 ttgttgtaat cctccagcag cgccagcagc gggtcctgca tatcctgtgc attggccgga
      841 tagaggtcca tcaccagacg ctctttaaac ccggcgaccg gcgcaagggc aaacagctgc
      901 ggttttacgt tttgctttaa ttcaaaaacc atacgtacgg tttgcgggtc aaattgcccg
      961 acgcgcgccg acttgatgaa cgggtcgtca gcgcggattt gcgcagccat ccccttgagc
     1021 accgagttca ggtttacatc ttcgatatcc accaccacgc gttcaggatt actcaacgcg
     1081 aactgcttat atttcagctg acgatttgat tctaccgtca cgcgggtgta gctggacgca
     1141 ggccaaacgc gcaccgccac gacctggctg accgcagcca ggctgacctg acttacgctc
     1201 aatagccaca tggcacccgc gccttgcagt aaacgacgac ggctgattgc agtgttggat
     1261 cctgacatgc ctctcccgag caaaagaaat ctaagctgtg taacaagtaa acgaccaatt
     1321 tgaccggaaa ctttagcgaa agacgcataa tctgtcatct ataaaacggt aaacattctt
     1381 tttatattca cggtattact gataaaaaac tcgctcccgc ataaaattta cacttgcacc
     1441 ctgcgaaaaa acagaataaa aatacaataa tttcgaataa tcatgcaaag aggtatgccg
     1501 tggtaaagga acgtaaaacc gagctggtcg agggattccg ccattcggtt ccctatatca
     1561 atacccaccg gggaaaaacg tttgtcatca tgctcggcgg tgaagccatt gagcatgaga
     1621 atttctccag tatcgttaat gatatcgggt tgttgcacag cctcggcatc cgtctggtgg
     1681 tggtctatgg cgcacgtccg cagatcgacg caaatctggc tgcacatcac cacgaaccgc
     1741 tgtatcacaa gaatatacgt gtgaccgacg ccaaaacact ggaactggtg aagcaggcag
     1801 cgggaacatt gcaactggat attactgctc gcctgtcgat gagtctcaat aacacaccgc
     1861 tgcagggcgc gcatatcaac gtcgtcagtg gtaattttat tattgcccag ccgctgggcg
     1921 tcgatgacgg cgtggattac tgtcatagcg ggcgtatccg gcggattgat gaagacgcga
     1981 tccatcgtca actggacagc ggtgcaatag tgctaatggg gccggtcgct gtttcggtca
     2041 ctggcgagag ctttaatctg acctcggaag agattgccac tcaactggcc atcaaactga
     2101 aagctgaaaa aatgattggt ttttgctctt cccagggcgt cactaatgac gacggtgata
     2161 ttgtctccga acttttccct aacgaagcgc aagcacgggt agaagcccag gaaaagaaag
     2221 gcgattacaa ctccggtacg gtgcgctttt tgcgtggcgc agtgaaagcc tgccgcagcg
     2281 gcgtgcgtcg ctgtcattta atcagttatc aggaagatgg cgcgctgttg caagagttgt
     2341 tctcacgcga cggtatcggt acgcagattg tgatggaaag cgccgagcag attcgtcgcg
     2401 caacaatcaa cgatattggc ggtattctgg agttgattcg cccgctggag caacaaggta
     2461 ttctggtacg ccgttctcgc gagcagctgg agatggaaat cgacaaattc accattattc
     2521 agcgcgataa cacgactatt gcctgcgccg cgctctatcc gttcccggaa gagaagattg
     2581 gggaaatggc ctgtgtggca gttcacccgg attaccgcag ttcatcacgg ggcgaggttc
     2641 tgctggaacg cattgccgct caggcgaaac agagcggctt aagcaaattg tttgtgctga
     2701 ccacgcgcag tattcactgg ttccaggaac gtggatttac cccagtggat attgatttac
     2761 tgcccgagag caaaaagcag ttgtacaact accagcgtaa atccaaagtg ttgatggcgg
     2821 atttgggtta agcgatgaaa atcgccggat gcgacataca taacattcat aggcatgata
     2881 agacccacat gcgtcgcatc aggcaattac gtttatcccc gtgaactaaa caacgccgcc
     2941 agaccactgc gccgctcggt gcgcgtggca attgccgcac ttaatatgcg ctcatcggca
     3001 tacagcgaca gacggcgacg cgcgcgggtc actgcggtgt aaaccagctc tcgcgttact
     3061 accggcgtgc gttgactcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat
     3121 ttatgtaccg tcatcgccca cgtcgtttcg tgctctggca ggcgactcgg ttgcacagac
     3181 ttaatattgc cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg atccagcgca
     3241 ataccaatat cgccattaaa caacccaagc gcgctgtcat tacgggcaat catcactggt
     3301 cggccttcgt accaacgaga gtgcggatga cgatgaattt tgcgcttctg ttgcataaac
     3361 tgctcaattc gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa
     3421 agctggtact cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcagcaga
     3481 tccagataac gcccgtaacc cgcaagagct tcctcaagca tcgcaatata atcttcgcca
     3541 ctctgtaaaa gccgtttttc gatatcagta aaatcctgct gaaaaacggt tttcactgcc
     3601 gttttatcac cacggttaat cgccgcagct aactgaccaa tgccagaatc gctgccgaaa
     3661 cgatagcttt tttgcagcag gcagaggctg tcgcgcaaag atgccgcttc tgtgccagtt
     3721 cctgccggaa cgtggcttcc cgtcaggcgg ctcaactgcc cggcacgctc ggcggtaaag
     3781 cccgcgttgg cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt
     3841 tgatcacgat cgccgagaaa gatcactcgc gcatgatcgg gcaaggcgtc gatcagtctc
     3901 gacatcatag gaagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc
     3961 gggttaccgg catgatgacg taaacgctgg ctacccggct gcgcacccag caatcggtgc
     4021 aaagtgctgg catcttccgg aatgcgtttc ttttgttcat cggtcagcgg taattgtcgc
     4081 aaagccttgc cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga
     4141 cggatacggc agcgttcgcc gtcggccatt tgaattaacg ctgccagtaa cttcgctacg
     4201 gtggtcgttt taccggtgcc aggaccaccg gaaatcaccg agatccgccg tgtcagcgcc
     4261 actgccgccg caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg
     4321 gtttgcgcca gtagagcttc atcaacctca atggcatgat tcacttcgtt gaaaaagcgt
     4381 gccactgtgc gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc acagaggatc
     4441 atcggcgttg gttcatctcc ccggctgacc gcttgtgaag ccagcaagca ttcttcccaa
     4501 ttttgtagct caccgatttc actgacacag gtcgccaaca gcggatgcga tgcctcgtta
     4561 ttttccagtc gtgaaagcgg caaacaaacg tgtccctctc cggcatcatg acttaatagt
     4621 gccgccgcga gggtgacggc aggatgttca tctcccgcca cggtcagggc aaactgcacg
     4681 tccagcgggc gtagctgttt gtgctccaca gcttccagta attgcttttg caatttcatt
     4741 acgcctcctc cagggtcata ccggcaaaca tctcatccat caggtcaatc aacccggcgt
     4801 tgggtcgggt cgcgtagatc ccttgttgcg gatgttcttt atcaacgcca cgcaggaaca
     4861 gataaataac gccgccaaag tggcgctcat agtcgtaatc agcaatacga tggcgcagat
     4921 aacggtgcag cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcattg
     4981 ccgctgccat agcctgttgg gtgtaagccg aactgtcttc acccaaccag ttggatttat
     5041 agtcgagcag gtaataacgc ccttcgtggc ggaacaccag atcgataaag ccttttaaca
     5101 tgccacgtac ctgcatgaac tccagcggcg ggcagccagc ggatagcggg tcaaactggc
     5161 gaattagtgc atcaagctgg ctggcgataa gcagttcact aatcggcagg taaaactcca
     5221 tctccacctg tttatcgcga tcggaaagct gattcaggct aacacccgtt tcattgagag
     5281 gtgcctggag gacagccgtg atccactcag tcaacaccgg ttcccactgc ggttcaaagc
     5341 caccgagctc cagtttttcc tgtacccagt tcgggtcaac cggctgggta aaatcgaggt
     5401 cttcaaacaa actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac tgatgcggtg
     5461 ttaacgtcgg ttcttcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca
     5521 tcagatcctg agcgataccg tgaccacgct gctgcaaacc ggagtagctg gtgacacgcc
     5581 agttatcgcc gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt aatgcatcat
     5641 taacctgcca gggctggtta tcaccaattt gtgccgtttg ccaggcaata tcatcatcgc
     5701 acaacgcttc aatacaggta cgaagccctg ccgcatcttg cggttctcct ttttgcagca
     5761 aacgcccgag cgcactttgg tggacgtcgg tatcaccttt tttatcgcca cgacggcgca
     5821 ccagcggtgc aacgccgaga ctgcaatgcc aaaccgaacg cgtcagcgcc acgtaaagta
     5881 aacgcagatc ttccgccaga cgttcggcct ccgcgaggtc gacgctttct ggcgcagcat
     5941 taagatccag aacggcctca aacgagtggc gatcgtgata aaacgcctga tcctggacgc
     6001 ggaaattggt aataaatggc aaccagacca gcggatattc cagccctttt gatttgtgta
     6061 tcgtgacaat ctgcaccaga tgtttatcac tttcgaggcg catttgttgg ctggaggcat
     6121 tactgtctgg ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca
     6181 gctgcgttcc ggcttcttgt agcagttcgc tgacatgcaa gatatcggta agacgccgct
     6241 caccgcctgc cgttgccagc aagttttctg caatgttacg cgccgacatc agcgcccgca
     6301 gcatcggcat aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcgacga
     6361 ccgcatccca cgcatgttcg tcattattca gcgtttcaat atccagcgca ttcagcccca
     6421 tcattgacgt tgccagcgca ctgcgcaggg tgttctctcg ttcgggtgtc atcaccgcct
     6481 gcaacagcca aagcatctcc tgtgcttcca gagtttcaaa aacactgtcg cggttcgaaa
     6541 ggtaaacgga agggatttcc agcagcgtta aggcatcgcg cacctgagcg gcctcctggc
     6601 ggctgcgcac cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca
     6661 tcagcaacgc ttcgccgcgc tgtccggctt gtaaccagtc gcggatttgc gcagcacata
     6721 cctgcgccat ggtactttga tagtcgccaa cgccgcagct ttcgccttcc atcagccaca
     6781 ttttcatcgc aggctgcgtt tcacctttaa acacaaaacg taacgcctga tttttgccgg
     6841 cagatttcac aggaataaac ggtatttcac gaaacatgaa cgcgtcatca gtctggctga
     6901 aaagtttgtt aacgctgttc accattcccg gtgcggaacg ccagttggta tctaaggtgt
     6961 agtgagcatg aacttcgcta cgcgccttca tataagtgaa gatatccgca ccccggaatg
     7021 cataaatagc ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc tgatggtgcc
     7081 agatacggcg aaaaattcga tactgctggg ggtcggtatc ctgaaattca tcgatcattg
     7141 ccaccgggaa tcgcgtacgg atcgccgctg ccaatacctc gccgctttca ctacgcagcg
     7201 cggaatcgag ccgacttaac atgtcatcaa aacccaattc gccacggcgg cgtttttcac
     7261 gcgctactgt ttcgcggatc tcagccaatg cgcgagtgat caccagatcg cggatcgaca
     7321 atggttctgc aagcagttgt tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc
     7381 cggctttcgt gcgatcttct aagaaacgct gagagaattt ttccagcgac tccggcaact
     7441 gatagctgtt ggtctcttct tctgcccagg cgctgatctt ctcgatccat ttagcctgat
     7501 tgctacggtt aaacttgcgt cgatcaatac cagaagattc gatcagcgca tccagttcac
     7561 ccactgcgtc gcgccactgc tgttttacgg catcaatacg cgccacaatt tgcgcgtggc
     7621 gggaagccag cgtttcatca tcggggggcg gtgctttgat aaccggcgct tcgccttgca
     7681 gataacgatt aatatcgcgc agcaacgcct gcggcccttt ccaggtttca aagacgacct
     7741 gggcaatttc acgcggcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt
     7801 agcgtagcag agactcatct tcaatcagct gctgctcaaa cagcatgccg gattcaaagg
     7861 cattcaggtt gagcatgcgc tggcaaaagc cgtgaatagt aaagactgcc gcttcatcca
     7921 tctgccgctc ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca
     7981 gcaggcgttt gtacagtgga ttgtcggtgg tttcacgcag acaggcgatg cgcaactcgt
     8041 ggatattgct acggatacga ccacgcaatt ctgccgtggc agcctcggta aaggtcacca
     8101 ccagcagttc ttcaacggtc agcgggcggg gaaaggcggc agaaccgcct agtccaagta
     8161 acaggcgcaa atagagcgcc gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa
     8221 tcaggcgctc gccctgtaag ggcaagcgca aaggatctag tgtctcggcg acatcactca
     8281 ttcttttcac tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc
     8341 cagccttcag gatgcacata ttcggctttc ccgttctggc tgccggaaat ctgcgacaga
     8401 atagccatac cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc
     8461 gtcagcagtt ttatctgggc cacgatttta tcacgcgaat agaagcgcat attgccgcga
     8521 tcgaaatctt tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc
     8581 tgagtaatta ccgcctgctg gatttgcgca aactcctctg gcttcatcgc ccgcaatttt
     8641 gcctctgcgg ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca
     8701 ttgctttgca aaaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac
     8761 acggcatagc ccaactgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc
     8821 tgccccaaca gagaactata cgctgagctg gtgtattcat cgtagccagt cggtacaaat
     8881 atcgctgcca gtgcggagtc ggtgctgtta ccagcttttt caaagatgac ggattgtttt
     8941 ttatcgacca cgacatcttt gttacgacac cactctgaac catcagcgcc caactgtttt
     9001 tgcacatggc gtgccagcgt tgttgcctgg gcttcagtca tgttgccgat aaccataaac
     9061 tctggtctag cccctgattt taaggcgtcg cgataggcca gcacctcttt caacgtaatg
     9121 gagggcaaaa ttttgcgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc
     9181 gcgggcataa tcgcctgctc aaacgctttg cccttttctg cggaatccat catctggtta
     9241 taccaggact tcgcctgctc aagctgatct tccgtagcgg tatagctaaa gtagccctcc
     9301 agcagagcct ggaacagctg cggcaggcgc tgagtgtaac cattggcatt aaccataagg
     9361 ccgttgttag cattggtgga aaaacttatg ccaccaaccg acgcctggtt gcttaactga
     9421 tcaagcgcca gccctgcgag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg
     9481 tccatggctt tcggattacg caaaatcagg ctgacatcag ctttgggctc actggcaaaa
     9541 taacggcttg gcgcatacac cacgcgcaga ttcgactcat caacaatcaa ctctggatgg
     9601 tcgtatttct tctctgactt aatcagcgag aaatcgtcag gaatataggg gttaagctcc
     9661 ggtaaggaga gcgcaatatt ggcggctttt ttctgccagt ccgcgaaagt ttgttcgctg
     9721 attttatcga cttgatacgg cgcatcgaca aaataagccg ttttattgtg cggctctttc
     9781 gggctgatat accagatacg cgcattctgc ggcgtcatca tcgccagacg ttcttttact
     9841 gctttagcat cgtaccgatc ggcaatattg actgcatcca gtgtatgctc aacaggaacg
     9901 cgaatcatgg tatcagccag ccattcgacg taatccatat cacgggtgat tgacggataa
     9961 cggaagtcga tatccagcac attcgccagt tcatcgaagt attgtttatc gatccctttt
    10021 tcacgtaaca gattgaggta gctaaaaatg gccgccacaa cctgatcgcg attcgccaga
    10081 cctttatcgg ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga
    10141 tcggaattgg cgctaatacc ctcaactaat ccctgctttt gcagccagtc agaaagtgta
    10201 cctgggctgc gattaccaat cagataggta atcaattcat ccgttttact acggaacttc
    10261 gctgaattgt tatcgatgcg aaactcaacg cgcagaactt tacgcggcaa cgccgggacg
    10321 taatgaatga taatgccctt ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt
    10381 ttgctctctt tgttcggcac gcgaccaaag gtgtccgccg ccattttcgc caactccggc
    10441 aacggtttat tgctgtaaat aaccgccttc atcagattgg cggaatagta cttctcgtgg
    10501 aaatctttca gcgcctgctg taccggatta cctggtttgt cgcttaaagt ttcgaggtta
    10561 ccaccagaaa actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgtgcc
    10621 atgcgcatcc cgtcacgcgt acgcgccatg gttaattcgg cattcactgc attacgttca
    10681 cgttcggcgt atttcttgtc gagcaagggt tctgcaatag cgtccgccag gcggtctacc
    10741 gcaccgggca aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggtgccgtg
    10801 ctggcattgt gactgccgcc gtgcattttg agatattcgg ccagactgtc agcctgcggg
    10861 tactttttcg accccatcag actcatatgt tcaaggtaat gtgccagccc ttggtacgcc
    10921 tcaggatctt ccagcgaccc aacgggcacc accagcgccg agagcgattt aactgcctgc
    10981 ggatcagaaa ccagtaagac caccataccg ttatccagac gtatagcctg atactggcgg
    11041 ttatctttat cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga
    11101 cttaagggtg cccagagggc aacaaacaac aataatgctt tgaaccagat gctgcggggc
    11161 attcacggac ctcataatca actttatttt ctggccagat tcaacacgtt aacgcaaatc
    11221 atctgccaga atttaatctt gtgctgcacg agtcagccta tgtctatata accatcagtc
    11281 cgtgactggt gcgcatcata aagtaagcgg atagattgcg caatttttat acaatactca
    11341 tgactgatta aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa cgatagcctc
    11401 cattgtctct ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc
    11461 acgcaccatc atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgcaacgt
    11521 ggaatcgtca tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc
    11581 gccactttca ggtaacacca gcaatggcgc ggacattcct tcacgatacc cttcaatcag
    11641 ctgtgagagg taatgtaaag cctgttcggc tgcaagcggc ggaaaacgcc actcgccgtc
    11701 tttgcgtaga aaaaggcgac tttcaccatt accaccgctg gcacagtaga caaggtgttc
    11761 cagccaaagt tgcattccct gcgccacact taataaagag ggacgccagc gcaacaggcc
    11821 atccggctgc acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcaat
    11881 ctccatactc tgccctggct ggcgacaggc aatgactctg tcggcaagtt gctgcatctc
    11941 ctgacactgc gtttcccaga aaatttcacc aaaggcacca tacggcaaat cacctgccgc
    12001 ccggaatcgg cggaacaagc gttcggcatc atcctgctca accagtacat ttaataactg
    12061 ctggttgatt tgataacgac taagcccttc aagaataaaa ggttcggtgt cggggatttc
    12121 gctgtcttcg gtacggaagt tcacctgcaa acgcatctgg aaaaatgccc gcaccggatg
    12181 tgcccagaat cgttgtagcg tttccagcgg cacggtttcc ggtaaggtaa atggcagcgg
    12241 ctgaacaaat tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc attcacgagc
    12301 atagctttgt cgttcgcctg gctggtagtt ttgtggatca aacggcattc gggtatggag
    12361 gcaagtaaga tgcgctttta cccttgcctc gctttcatca cagttgagcg cttcatcgcc
    12421 cggtagataa tggctttgcc cgatgtagtc aatcagttcc tgcaccagta ccgacgggaa
    12481 acgttcactg ttatcctgaa tggaacgccc gatatagctg atatagagtt tttgctgcgc
    12541 ggaaattaac gcttccagga acagatagcg gtcgtcatcg cgacggctac ggtcgccacg
    12601 cttcggtttc tggctcatca gatcaaagcc caatggcgca agctgacgtg gataaacgcc
    12661 gtcgttcatt cccagcaggc aaaccacttt gaacggaatt gaacgcattg gcatcagagt
    12721 acaaatgtta accggtccgg cgagaaaacg ctggctgata cgttcttgat ccaggcgcag
    12781 tgccagttca tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc
    12841 ttcggcgata atcgcctgcc attgttgttc gatcagcgtc atcgccgctt cggtttccgc
    12901 atccggcagg aaaaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttccagcgg
    12961 acgctcctgc gccagcccgc gacgccagat atttagctgc attagcagtg aagccagatg
    13021 ccccaccagt tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc
    13081 gccctgcgcg ctctccatcg cgtagcccag caacatgcgc gtcaggccaa accgccaggt
    13141 gtgttgaccg gtagcgggaa gttccagctc gcgaacgttg tcgtcatcta tcccccaacg
    13201 aattccggat tcgttgaccc actgacgtaa ataacgcagc ccttcttcgg tgatgtcaaa
    13261 ccgcgctgcc agcaccggca catccagtaa tgccagcacg tcttccgaca caaagcggct
    13321 gtctggcagc gataacaggc tgataaacgc ttcaagtaca ggatgcgact gccgcgcccg
    13381 acggtcggaa atggcgtaag gcaggtaacg atccgcaggt gcgctaccaa acacagcctg
    13441 aataaacgga ctgtagctgt cgatatcagc caccatcacg atgatgtcgc gcggagtaag
    13501 tgtcgggtct tcctccagca tcgccagcag gcgatcgtgt aaaacttcaa cttcacgctg
    13561 cgggctatgg caaacgtgga aggtgatact gctatccagt ggatcaagcg ggcgtttgtt
    13621 atcgctacgg gaaaactctt cgatgttcac accagcaacg gcgcggtttt ccagttccag
    13681 aatgtcagac tgaatattat gcagcaggtt atctggcgtc acatcgacaa aagcgtccag
    13741 ctcctggctg ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa gcttgcccca
    13801 tgaagccagc agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga gctgcccggc
    13861 attttcgctg tcgcgaaata gcggtaattc gcgatcttca aaactgtgtc ggcgctggcg
    13921 agtcagtagt ttcgccagat aagcaggatc tttaatgtcg ccccagtaat aacggcaggg
    13981 gttggtaaac aggagatgga tttcaatatg tttacccagc gcctgtagcg cctggagata
    14041 aacaggcggt aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc ccggcgggca
    14101 ggtcgtcgcg gactccagcg tttcgataaa gcgctgatag agattggcgc ggtgccagcg
    14161 cggttgccca agttcgtcgg tatattccac caacgccttc cacaacggcg cttgccaggc
    14221 ctgtgcttct cccaaccctt ctaccagatg tcctgtttcc cactgtgcca gccagtccgg
    14281 acgatagacc agatactggt caaacaggtc cgccgctttt gaagaaagct ggaacagttt
    14341 tcgcttgtca ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccaa
    14401 cagttgcggc agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcgctctc
    14461 tttggggatc tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg
    14521 aaaatcaatg tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgtgc
    14581 cataccggta ctttgcacca gaatcatctc tggttcgaaa ggatcgtcca gccgttcgcg
    14641 ttcgacaata aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct
    14701 taacatagcg gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc
    14761 tctgcccccg ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg
    14821 cattcggttg acctgccagt tagcaggtgg cgaaatcgcg tgcagttgcg tttgctgcca
    14881 gccatgccgc cagagctgtt ggtactggtt tctgctggca agactgttca ttaatgttcg
    14941 ctggaaaccc gataacgcag tgacaatcat caccatcaac accatcgcca acattacctc
    15001 cggcagacta aagccttgtt gattcctcag ggaagctgac ataacgccct ctctttcagc
    15061 ggacaaaaat cgctccagcc gcgtggcgaa aaaaaaatgt ttccatcgat gacttcgcct
    15121 gttcgccaca acgaaacgcc ttcataacca gcaatcaata aggcttcatt atctgccagt
    15181 aaacgcagac aaacccgggc accggtggca gcatacagta agcactgaac tgcgggcagc
    15241 gtctgccagg agtgcatttt tccccactcc agcgccgact gaacgatggc ctggcggcgc
    15301 aatgactgac tttccatgct cacgcgagaa gcaaaactgc gatcctgctg actcattcct
    15361 tgcaatagca agctacccaa aatcagcagc atcaggacca gagccagtga cgaaacaccc
    15421 ttttcgcggt tcacaggttg gatcctgtca cgctatagct ggcatccacc acggtttgcg
    15481 gatcagcctt gctggcagcg tgcatattaa ccgtcaacac cggcgagaag ccgctgacat
    15541 cctgacgtac gacctgaaaa gtgtcgataa tgatggcatc cggattagtg actttatccc
    15601 agcccttacc ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac
    15661 gaaatccaat ctggtcggac tcttttaccg gttcgcgatc ccagatacca ttactgttcg
    15721 catcccactg cacaatgaca cagtcaccct gtccgacaat ttccagccct tcgcccgtac
    15781 agctgccatg gcaataaccc gccctctgga gatgcttcgc gacggtaaat acccgcagcc
    15841 agatttcatc ttccagcgct agcttacggg tgttcgttaa actttcacgc tgtaacgcag
    15901 gcagaaagcg tgcagccccc agcaacaata cgctactgat cgccatagca atcaacactt
    15961 ccagcagaga aaaaccttgc tcttttacag gcatccttct gtttctcctt gctgacaaag
    16021 ccggagtctt ccccacggcg aaaccactag ccaccactcg cccgttgagt ttttgaagcg
    16081 aatatgcccg gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc
    16141 gctcatttcg acttcgggcc agcgcggcac aaagaccaat ggtgaactgc catgacaggt
    16201 attcgcccca gcaacggaac tcacaaggca ccataacgtc ccctccctga taagactgat
    16261 actgtggtcg cggttatgcc agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc
    16321 ctggctggcg gtttgccaaa gccgttgcga ctgctgccag tattgccagc cataaagccc
    16381 acttgcgctt agcatgacca gaatcagcat cgcgaccagc gtttcaatca gcgtataacc
    16441 acgttgtgtt ttcatgccgg cagtatggag cgaggagaaa aaaagacgag ggccagtttc
    16501 tatttcttcg gcacatcttc cggactattt acgccgttgc aggacgttgc aaaatttcgg
    16561 gaaggtgtct cgaagaattt aacggagggc aaaaaaaacc gacgcacact ggcgtcggct
    16621 ctggcaggat gtttcgtaat tagatagcca ccggcgcttt aatgcccgga tgtggatcgt
    16681 atccttcaat ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt ttacgtttga
    16741 taatcaactt cggcagcgga cgcggttcgc ggcttaattg cagatgagtt tgatccatat
    16801 ggttgctgta gagatgcgtg tcgccaccgg tccagacaaa atcacccact tccagatcgc
    16861 actgctgcgc catcatatgc accagtaacg cgtagctggc aatgttgaac ggcaggccga
    16921 ggaagacgtc acaggagcgc tgataaagct ggcaagagag tttgccgtct gccacataga
    16981 actggaagaa tgcatggcac ggtgccagcg ccattttatc cagttcgcct acgttccacg
    17041 ctgaaacaat aatgcggcgg gaatccgggt cgtttttcag ctggttcagt acagtagtga
    17101 tctggtcaat atgacgacca tccggcgttg gccaggcgcg ccactgttta ccatacactg
    17161 gcccgaggtc gccgttttca tcggcccatt cgtcccagat ggtgacattg ttttcgtgta
    17221 gataagcaat gttagtgtcg ccctgcagga accacagcag ttcgtggatg atggaacgca
    17281 ggtggcaacg tttagttgtc accagcggga atccatcctg caggttaaaa cgcatctgat
    17341 gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg ccttcgtcga
    17401 gcactttttg catcagttct aaatactgtt tcatggttcc tcaggaaacg tgttgctgtg
    17461 ggctgcgacg atatgcccag accatcatga tcacacccgc gacaatcatc gggatggaaa
    17521 gaatttgccc catgctgatg tactgcaccc aggcaccagt aaactgcgcg tcgggctggc
    17581 ggaaaaactc aacaatgatg cgaaacgcgc cgtaaccaat caggaacaag cctgagacag
    17641 ctcccattgg gcgcggttta cgaatataca ggttgaggat aataaacagc accacacctt
    17701 ccagcagcag ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa gtgtcgaaaa
    17761 tggattgcca ctgcggattg gtttgcagca gcaaaatatc ttctgtacgg gagccaggga
    17821 acagcatggc aaacgggaag ttcgggtcaa cgcggcccca caattcaccg ttaataaagt
    17881 tgcccaggcg cccggcacca agaccaaacg gaatgagtgg ggcaataaaa tcagagacct
    17941 ggaagaagga acgtttagta cggcgggcga agataatcat caccacgata acgccaatca
    18001 ggcctccgtg gaaggacatg ccgccgtccc atacacggaa aagatacagc ggatcggcca
    18061 taaactgcgg gaaattgtag aacagaacat aaccaatacg tccaccgagg aagacgccga
    18121 ggaagcccgc atagagtaag ttttcaactt catttttggt ccagccactg cccggacgat
    18181 tcgcccgtcg tgttgccagc cacattgcaa aaatgaaacc caccagatac atcaggccgt
    18241 accagtgaag cgccacgggt cctattgaga aaatgaccgg atcaaactcc ggaaaatgca
    18301 gatagctact ggtcatctgt caccacaagt tcttgttatt tcgctgaaag agaacagcga
    18361 ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc acaaggggac
    18421 ctggctggtt gccggatacc gttaaaagat atgtatatga tccgcgctat aaccccccgc
    18481 gaatcaaccc gcccatgccg cgacgctcca taaaggctgc aacctgatgg cgaacttcgg
    18541 tcgccagttg cgcttccaga ctacgctgcg caagattttc tgcttcagca aaatcaatgc
    18601 gccgcagcag gtattttacg cgcgctacag aacgtccgtt catagacaga tggcgatacc
    18661 caagcccaat gaggattgcc acgcacatgg gatcgcccgc catttcaccg cacaaacgga
    18721 gatcgattcc atgtatttcc gcttcccggg cgatcatcgc cagagctcgt aacattgcag
    18781 gatgaagact gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg gccaggatgt
    18841 attgagtcag atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc agatgcggca
    18901 gcataaatac cattgacggc acttccagca tgatgccgat acgtggtttg ggaatttcgt
    18961 aaccgatcat ctcctcgact tcacgtccgg cacgttcaat caggcggcgt gcttcgtcaa
    19021 cttcatcgag gcttgtgacc atcggcaaca gaatattcag gttgcccgta gcggcattag
    19081 cacgcagcat cgcccgcacc tggatcaaga agatctccgg ctgatcgagc gtaatgcgaa
    19141 tcccacgcca acccaggcat ggattctctt cgctaatcgg catgtaaggc agctgcttat
    19201 ctgctccgac atccagcgta cgcaaggtga cgggtttatc attaaacatt tgcagcatcc
    19261 cctgatactg cgccacctgt tcttcttccg acggaaaacc actttgcagc atgaatggga
    19321 tttcagtgcg ataaagtcct atgccatcaa tacggctgcc cagtttttct tcatgttccg
    19381 ggcttaaacc ggcattgagc atgactttaa tgcgctcgcc gctttttaac tgggcgggta
    19441 aattgacgtc atcttccgcc agacggctaa gctcgatctc ttcactaatt agccgctgat
    19501 attcttgcag cagtaccggt tccggatcga ccagcaattc accgcgataa ccatcgacga
    19561 tcagcgtccg acgatgcagt accgaaggct gaatatccgc gcccatcacg gtaggtatcc
    19621 ccagcgcacg taccatgatc gcagcatggg agttggcagc gccatcgcgc acgacaacac
    19681 cgactaagcg atcctggggc agctcagcaa gcgttgtcgc tgacagttca tctgccacca
    19741 gaatgaaacg ttccggccag gcgttcggcc cttgattagc gtcatcaaga tgaaacagca
    19801 atcgctgacc cagcgcacgt aaatcgccag cccgctcttt gagatagtta tcgcttagcg
    19861 cggcaaactg ttcggcaaat ttttcaatga ccgtttttac cgcccactct gccaccgagc
    19921 ctttatcaac ctcggcaaac aattcgcgac gcagccgggt atccgaaagc aggtgtgagt
    19981 aaagatcgaa aatagccgcc gtttcttttt gtgcaccggc agcaaagcgt ttgctatagc
    20041 ggcgaaactc gttagccgcc tcttccaacg ccccggtcag tcgttcgcgt tccagagccg
    20101 gatccagcgt tgatgcctga tacacctgtt ccattaaagg taacgtggca tcctgccagc
    20161 cttcggcaat cgccacacca ggtgctgccg gtaatgcacg gattcgcgtc tggcgatatt
    20221 gcccaaacaa ggcagtcaac tgcgactgag aaagaatagc tgccatctgg gtggcaagcg
    20281 tcaccaggaa ggattcttca ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca
    20341 gtacaccaag caactggcga cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt
    20401 cttcttttac ggaggggatg tatttgaagc tggggtgctt ttgcgcatct gcaaggttta
    20461 tcggttccgc cagcctgcca accaggccga cgatcccttc atcaaacgcg agcgttacag
    20521 tgcgaccgcg tggttttttc agtccccggg tcgccatcag gtagtaacaa cgtcgatcat
    20581 gatcggccag gtagaccgaa cagacctcgg tatccatcgc aagacagatg tcggtaacca
    20641 gaatatttaa cgcctcattc aggcgtggtg cgctggctac cttttcgact atttcgcgca
    20701 ggcgagtgag cataattggc gtgacttaac ctcttttacg tcgataagca gatgcgtttt
    20761 gtggttttgg cgtattttcc tgcagtgaca tcaccacact cgcgaactct ttcattaccc
    20821 tacggtagac atcacgttta aatgacacca cctgtctgac tggataccag taacttaccc
    20881 atcgccagcc gtcaaactct ggtgtactgc tggtttgcat attgatttct gcatcgccgc
    20941 tcaccagctg caagagaaac catttttgtt tttggccgat acaaaccggc ttcgtgtccc
    21001 aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa gcaaggattc
    21061 gaacgtcttt gcggcttaat cctacttctt caaacaattc acggtacatc gcctgctctg
    21121 cggattctcc ggggttgatt ccgccttgcg gaaattgcca ggagtgctga ccaaatcgcc
    21181 gggcccacat tacctgcccc tggcgattac aaatcacgat acctacgttt gggcggtagc
    21241 catcgtcatc aatcaccgga ctacctcaaa ataaagcttt atatacgaat gattgtttca
    21301 tactccagga agacggtaaa ccactctctg cagggcatta cacactaata acaattgaat
    21361 aactcacagt tatgtgcaga gttataaaca gaggaagggg tggatagccg tttttattca
    21421 ccttttctgt ggatagagtt gtgaagaact acggaattac tacgggaaaa cccggagcat
    21481 tctgaataag acagatacag agattttaaa atattcatta tcagtcaatg cattacatgt
    21541 gatttgagta ataaaaaaga gtaattcgtg acccagatca cacctctcat ttacgggttg
    21601 gcgaaagatc aaccaatgcc gtatttatcc acagaatgtg ccactaagtt aagcactgaa
    21661 ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag tagtgaaggt
    21721 ttttcacagt tatcccagct ttctgtggat aacatggtgt aagatcctgt ttattttcag
    21781 tgaccagatt tggaaaaccc gttgcagtgt tgcgcaactc gattaccggc aacctaaaaa
    21841 gcaatataaa tcagagaatt gaacaacgca tgtggaaaaa gttacactgc gaatattcgg
    21901 cacttaattg ctgtttgttt tttaaccaag gtatcatgac atgtcccaac ctcgcccact
    21961 gctctctcct cccgaaactg aagaacaatt gttagcgcaa gcacaacaac tttctggtta
    22021 tacattggga gaactggcgg cacttgccgg gctggttacg ccggagaatt taaaacgcga
    22081 taagggctgg attggcgtgt tactggagat ctggctaggt gccagcgcag ggagtaaacc
    22141 tgagcaagat tttgctgctc tgggcgtgga acttaaaact atccctgtgg atagtcttgg
    22201 tcgtccgctg gaaacaacat tcgtttgtgt tgccccgtta acgggcaata gcggggtgac
    22261 ctgggaaacc agccacgtgc gccacaagct caaacgcgtg ctgtggatac cggttgaagg
    22321 cgagcgcagc atcccgctgg caaagcgtcg cgtaggatca ccgttgctgt ggagcccgaa
    22381 tgaagaggaa gaccggcagc tacgcgaaga ctgggaagaa ttaatggata tgattgttct
    22441 cggtcagatt gagcggatca ccgctcgtca cggggaatat ttacagatac gaccgaaagc
    22501 agcgaatgcg aaagcgctga ccgaagctat tggtgcccgg ggcgaacgga ttctgacgct
    22561 gccgcgcgga ttttatttga agaagaattt caccagtgcg ctactggccc gtcattttct
    22621 gatccagtag ccatcgcttt gacctgctgc tttccgggca tataattacc gcttcatttt
    22681 tttggcaggg ctttttagat gttatttgca tggataaccg atcctaacgc ctggcttgcg
    22741 ctcggtacac tgacgctgct ggagatcgtt cttgggatcg acaatattat tttcctttct
    22801 ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct ggggttggcg
    22861 ggagccatgg ttatgcgtct ggcgctgctg gcatccatcg cctgggttac gcgcctgacg
    22921 aatccgcttt ttacaatatt cagtcaggaa atttccgccc gtgatttgat cctgcttctg
    22981 ggtggcttgt tccttatctg gaaagccagc aaggaaatcc acgaatccat cgaaggtgaa
    23041 gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg ctatcgtgca gattatgctg
    23101 ctggatatta tcttcagcct cgactcggtg attaccgctg tgggtctgtc agatcacctg
    23161 tttattatga tggctgccgt ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg
    23221 attggtgatt ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt
    23281 ctggtgggct ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa aggttacatc
    23341 tacttcgcga tgttcttctc tattgcggtt gaaagcctca acctgattcg caacaaaaag
    23401 aatccgctct gatactccgt acgctctcct gcgggagggc gtactcactc ctgttaccct
    23461 cacagtttaa gattttcctg ctttcaagat atataacgtc ggtttataaa cagactatta
    23521 tcataggtgg gccaaatgag gataaccgga tgaaaaaatg ggcagtaata atttctgcag
    23581 tcggactggc gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg accaaagatg
    23641 gccgtatgat tttgaccgat ggaaaacctg aaattgatga tgataccggg ctggtgagtt
    23701 atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg
    23761 aacgttaaca aataaggtca gcatccggct ggccttaaga tttttctctt tcccttttcc
    23821 cttccctctg ccatttttat attccttatg tcgtgattat aaaaaggaaa cggctatgca
    23881 atatcaccgt ataccccaca gttcgctgga agtcagcacg ctggggcttg gcacgatgac
    23941 gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca
    24001 gggcattaac cttatcgacg ttgccgaaat gtacccagta cctccgcgcc ccgaaactca
    24061 agggctaacc gaaacctacg tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt
    24121 aattatcgcc tccaaagtga gcggaccgtc gcgcaataat gacaaaggca tccgcccgga
    24181 tcaggcgctg gatcggaaga atatccgcga agcgctgcat gacagcctca agcgtctgca
    24241 gactgattac ctcgatctct atcaggtgca ctggccgcag cgcccaacca actgcttcgg
    24301 caaactcggt tatagctgga cagattctgc gcctgcggtt tcgctgctgg atacgctgga
    24361 cgcactggca gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac
    24421 tgcatttggc gtaatgcgct acctgcatct ggcggacaaa cacgatctgc cgcgtattgt
    24481 caccattcag aacccctaca gcctgttaaa ccgcagtttt gaagtaggtc tggcagaagt
    24541 cagccagtat gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg gcacactgac
    24601 cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt aatacgctct ttagtcggtt
    24661 cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag
    24721 acgtcatggc ctggaccctg ctcagatggc gctcgcattt gtacgccgtc aaccgtttgt
    24781 tgccagcact ctgctgggcg caaccacgat ggagcagctg aaaactaacg tcgaaagttt
    24841 gcatctggag ttgagcgaag acgtattagc tgaaattgaa gcggtgcatc aggtttatac
    24901 gtatcctgca ccataagatt aacgccggtt aaaaccggcg ttaaatatta atgacggcgc
    24961 tgccagatcc acagcgccgt tattgccagc gcaaacagcg caccgaagcc aatgccaatg
    25021 ggcacgaccg ggatgcctac cattaccgcc agcgagtaaa tgcccagcat caacaacatg
    25081 gcgctgtttt cgccaaggtt ttgtactgca atcgcattcc ccgccccgac gcttttttta
    25141 ccccgctcct gtagcaacgc attgagcgga acgacaaaaa agccccccag cacgccaatc
    25201 agcatcaaca aagcataggc tggcagcagc tcgtgttgca gggagaaaat cagtaccacc
    25261 acgccaatca aaatcccggc tggcatacag cgtgacacgg tttccagcgt aactaacttc
    25321 gctgccgcac ctgcaccaac cacgatacca atcgctacca tcgcgttgag ataggtcggc
    25381 gtagcgttat cggtaatacc cagcgccacc ggtacccaca gcaccaacag gaaacgcagc
    25441 gtgacacccg ctccccagaa taaactggtg cccaccagcg aaaaacgcgt ttcaccattg
    25501 cgccatagcg aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag attccaggac
    25561 tgccccggac gcgccgccgc cagtttggga atgtagatat tggcaacgac cgcgccaccg
    25621 taggccagtg cgcatgcggc cagggcaacc agaatatgcc agtcagccag aacaccacca
    25681 gctacggaac cgagcaaaat cgccgctatg gtagacgctt ccattaaacc gttagctttt
    25741 actaacttac tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct
    25801 gcagcaccaa cacccaccaa cgtatagccg agaaacggat tgataccaaa gcagatactg
    25861 gctgcgccca gtagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta
    25921 tccgccacct gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc
    25981 aggatgggct ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac
    26041 agtagcgcat tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc
    26101 ttcgaccaca acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc
    26161 tacccagctt ttcaaagtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt
    26221 cagatagcga atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg
    26281 ttgtaactta tcgcgcatca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt
    26341 gctggcatcg ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag
    26401 ttgttccacc atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg
    26461 gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca atatcgccag tgtcatacca
    26521 gtcgcgctcc atttcgccgc gaacattctc ggcggtgggc acttccagta cacctggctt
    26581 ctccacccgc agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc
    26641 ttcgatacca ggaaccgaca acaggcgcgc atccattcct ggtagaatac gccctaccgt
    26701 accgggtttc gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt cggtcacacc
    26761 gtagccttca aggatgcgca ggccaaattt atcctgccaa agctgtttag tactttcttg
    26821 tagtttttct gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcaaa
    26881 gcgcgcgtag tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata
    26941 caccaactcc ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc
    27001 tgtgagcagt ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc
    27061 cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacgttcgc
    27121 cagaatgctt ttatggctat ggacgacccc tttcggatgg ccttcagaac cggaggtaaa
    27181 gaggatcagc gcctcttctt ccggctgctg ttttacctgt gccagacgcg gcatcagcaa
    27241 atgagcgaaa atccacactt tgtcggcagt ggtgacatct gcttttaaat cttccagata
    27301 gacccagcgc acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa
    27361 ctgacgggaa gtgaagatgg ttttgatttc agccgccgta atagcactgg tcaacccttt
    27421 taccccggcg gtgtagttca tcattgcggg aatgcggcga cgggcgatgg ccccaaaaat
    27481 cactgccgca ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact
    27541 gtatttttca aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc
    27601 cggggtaaag ttaacgtctt cgacacattt cttcccggct ccgaagcggt acattgcgct
    27661 cagtaaagat tcgtacagcg tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat
    27721 ttgatgcagc atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat
    27781 ctccacctgc gttggtggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac
    27841 cagacctttc aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac
    27901 cgttgcacca gacttcgccg cgacaaatcc cgcgccatcg tagattttca tcagcgagcc
    27961 agtggtggtg atgcgtcctt cagggaaaat caccactggt cgtccctgtt ccaccagacg
    28021 taccagatgt ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga
    28081 tttcagccaa cgcatatacc actgttggct tattgaggtg taaacggcaa ataccggacg
    28141 tacgggtaaa aacaatgcca gcagtatgcc atcaataaaa gagacgtgat taggcgtaat
    28201 tagaacgcgc tcgcccttca gtgccttggt gtcacccgta acgcgaacgc gatacaaaac
    28261 acggcacaaa tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta
    28321 aaagcgaatg gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg
    28381 aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac tgggtacgaa gagcgtaccg
    28441 aataatctca ccaatcaata cctctgggat cttgattgtg gtctgcacaa cgactcttcg
    28501 ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta
    28561 ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt
    28621 catgtgctca acccgaagtt gacttcacct atcaatacct ctgggatcac cactttagca
    28681 acctgaagcc aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa
    28741 ggtttgcaat ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacaatgaa
    28801 aacacgccgc cccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt
    28861 acccactaag gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca
    28921 gtcgccaccg tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctt
    28981 gctgtgcata gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg
    29041 gcgcagcaga ccactgaaac gatcggtctg gtcgttggtg atgtttccga tccgtttttc
    29101 ggcgcaatgg tgaaagcggt cgaacaggtg gcttatcaca ctggtaattt tttattgatt
    29161 ggcaacggtt accacaacga acaaaaagag cgtcaggcca ttgagcaact gatccgccat
    29221 cgctgtgctg cgctggttgt ccatgccaaa atgatcccgg atgccgattt agcctcatta
    29281 atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt
    29341 tgtattgctc tggacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa
    29401 ggtcataccc gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt
    29461 ctgcaagggt attacgatgc ccttgctgaa agtggtattc cggccaatga ccggctggtg
    29521 acatttggcg aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga
    29581 ggcagaaatt tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc
    29641 gtgctcaatg ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat
    29701 gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg
    29761 atggcgacgc aggctgccga actggctttg gcgctggcgg ataatcgccc tctcccggaa
    29821 atcactaatg tctttagtcc gacgctggta cgtcgccatt cagtgtcaac tccgtcgctg
    29881 gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa
    29941 ttcttcaatg gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc
    30001 tggtaacagc ggacggctgt tgtagttgga tgacattgat gcgccatatg cccctgtatc
    30061 atgcagtacc agataatcac ccgccttcac ttccggcaag gcgcgggttt cgacatttcc
    30121 cccttcctgc tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc
    30181 caccgttggt gcgtgttcca gagaacgacc atcagctgcc agggcactga tatggtggta
    30241 actaccgtac attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg
    30301 gcggctaccc atttgtttga cgctccgcac ttgagtaatt aatacgccag cctgcgctac
    30361 cagaaagcga cccggttcaa tttccagttt cacaggatgg cccaaatggc gggcgatttg
    30421 ctcacgcgcg gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg
    30481 ttgataagga atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaattc
    30541 gaggacctga cgcaccatag caccacatac ctgttccaga tgggcataat cgacgccaga
    30601 accaatgtgc atgtgaatgc cgaccagctg tagatgatga cgttgtatca cgtccagtgc
    30661 ggcgggcaga tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt
    30721 ttggctatgc ccgtgaccaa accccggatt aacacgcagc catacccgat gccctggcga
    30781 aacctgaccc agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc
    30841 actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacaa tatcatcggg
    30901 gtgcgtttgc ggattgtaac ccgccgccag tgcacgctct atttcgccta acgagacgga
    30961 atccactttt acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt
    31021 ctgtgcaaag cgcaccacat caaactgttt cagcgctgca atctgccgac gaataatttg
    31081 cgcatcgtag acccacaccg ggcagccaaa ttctgcgggc aaacgcagca gattttcggc
    31141 ggtgagatcg gtatcagtgc tgaacagtga atgtggcata acaaactcca gataagtgct
    31201 tttttatgat tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat
    31261 gcaaaaatga tatggattac cggattgcga gagagcgcta atggccgccg ttaacttacg
    31321 tcatattgaa atttttcatg cggtaatgac cgccggaagc ctgactgagg cggcacacct
    31381 gctacacacc tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg
    31441 gctgaaattg tttgagcgca tacgtggacg attacatcct accgtgcaag gactgcgtct
    31501 gtttgaagaa gtgcaacgat cctggtacgg actggatcgc attgtcagtg ccgcagaaag
    31561 tctgcgcgag tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc
    31621 ttttttaccg cagctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaacat
    31681 cgtgccccag gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg
    31741 actcactgaa acgctccata cgcctgcggg aacagaacgt accgaattac tctctttaga
    31801 tgaagtgtgt gtgttacctc cgggccatcc gctggcggta aaaaaggtat taacgccgga
    31861 tgattttcac ggtgagaact acatcagcct ttcccgtact gacagctatc gccagttgct
    31921 ggatcaattg tttactgaga atcaggttaa acgacgcatg atcgtagaaa cccacagcgc
    31981 cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggcgtt tcggtggtta acccgctcac
    32041 cgcactggat tatgcggcaa gcggtttagt ggtgcggcgg ttcagcattg cggttccatt
    32101 caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag
    32161 tgagcattta caagcgggat taccgaaact ggtcacttct cttgacgcta ttttgtcgtc
    32221 agctacgaca gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca
    32281 ggcagaacac tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg
    32341 ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct
    32401 tcggtaaatt gccccagaca cagttcttca aaaataatct gattaatttt tgcccgttca
    32461 tccgcttcag gaataagaca gttgatggaa aattgttccg tcagccgccc gcgataaaaa
    32521 tcctgttcca tggtgtaacg tgtacccagc agcgccacac gagtcattcc tgccccggta
    32581 attgcacatc cagtggcatc cgctatgtgt aagaaaggca gagagcaacg agactcaatg
    32641 gcatccgcca ctttatgcat cgtattggtg cataacacaa taccttctgc gcccgcccgc
    32701 tgtaagccaa gcgccgcctc agccagaata tccccggttt tgtcccactc accgcgacgc
    32761 tggcactctt ctatttcatg aaaatcgacg ctatgtagca gcacttgtgc agagtgaagc
    32821 ccaccaagcc gctgtttaat gccttcattt atcagacgat agtaaggaat agtggattcc
    32881 cagctcattc ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg
    32941 caacaaggct gaataaggaa cgagacaaac gcctcaacgg ccaggtgcct aatctctatt
    33001 aacgaaaaaa gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc
    33061 tcaacttttc gcctgccatc agtttgcgct cgatatgttc cagcgtgaca tttttggttt
    33121 ccggaatgag ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg tagagccaga
    33181 acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat
    33241 tcgacaccca gtttgtcgtc gttgagcagg tgatgccaaa atcgcggcat ttcagcggct
    33301 gaatttcaga gcacaagatc cacaccactg gcgcggcgct catcgcataa ccggcaatac
    33361 acatcatcgt catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa
    33421 actgcatcag gcagtaaccc agcaccagag tgcctaacgc catcacgctg aaaccaattt
    33481 tcagagccgg cttacgccct gctttatcta ccgtaaagac cgcaatgaag gtagcgaaca
    33541 taaaggtcag ccccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg
    33601 ccattttgaa gatacgtggc gcgtagtaca tgatgatgtt cataccggta aactgctgca
    33661 tcgcctgcaa caacataccg agaaatacag cacgacggac gttacggttg atcttaaaca
    33721 gtgcccaacc gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg
    33781 ctttttccga cgtatcgcgc agcatacgca atacttcttc cgcctcaata tgacgcccct
    33841 tttccgccag ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg
    33901 ctggtaaagc aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat
    33961 cggataaaaa cgccatcacg atgccgagag tgaccatcaa ctggtacatg ctgatcatct
    34021 taccgcgaac gttttcactt gccatttcag aaagatatag aggagcggtg taagacgcga
    34081 tcccgacagc aatgcccaac accacacgag cggcgattaa catctctacg ctggtcgcaa
    34141 aagcggaccc tatcgaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac
    34201 gccccaggcg gaacgacagc caaccattaa acagcgcacc aattgctgcg ccgagcatca
    34261 tgctactgac cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg
    34321 gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag
    34381 ctaccgaaac aaacatattc atacgccgca tatcccgcag aggacgtggc gttaaagcag
    34441 attccgtatt gatagtaacc attttttcct gccagcagag agtaagacat agtgaaaaaa
    34501 tacgtgaaca attcacacag gtgtcaggtc ggaaatagca taaatatgga ttaaattgct
    34561 acgacatgtc gttatgtga
//
LOCUS       DADSZD010000030        34092 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.030, whole genome shotgun sequence.
ACCESSION   DADSZD010000030 DADSZD010000000
VERSION     DADSZD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.030"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            35..895
                     /locus_tag="J1Z28_001326"
     CDS             35..895
                     /locus_tag="J1Z28_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716173.1"
                     /translation="MRTHPHLIKQTIDPRLINEINKRTQSNSEKLSNSDKLTYINDNY
                     CEITRNYTSFYGMNMLSIVLFLPIVILCFASVLFFIYDITFNFERYLDTWRYRADSEL
                     MLHIAYCAIFFMSFVFAAILNYFIFAPRHYPIRFNRKTGKVYICDYILNDMSHKSTYT
                     FWWHYPFYKHTKPEYKEYDWEQIQGIAFFAQNRHSSYSTIRCIVYESLDSTNIIDTFN
                     LITTESAIDALHMTNHKLWLWINSFMNFDDEFLEKSINTAPGIWGREVNWPEDMNKKS
                     MLSSMDEYIL"
     gene            complement(1086..1376)
                     /gene="ilvN"
                     /locus_tag="J1Z28_001327"
     CDS             complement(1086..1376)
                     /gene="ilvN"
                     /locus_tag="J1Z28_001327"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBA7716174.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(1380..3068)
                     /gene="ilvB"
                     /locus_tag="J1Z28_001328"
     CDS             complement(1380..3068)
                     /gene="ilvB"
                     /locus_tag="J1Z28_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBA7716175.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILHEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(3174..3272)
                     /gene="ivbL"
                     /locus_tag="J1Z28_001329"
     CDS             complement(3174..3272)
                     /gene="ivbL"
                     /locus_tag="J1Z28_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBA7716176.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            3673..4857
                     /gene="emrD"
                     /locus_tag="J1Z28_001330"
     CDS             3673..4857
                     /gene="emrD"
                     /locus_tag="J1Z28_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBA7716177.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(4865..5362)
                     /locus_tag="J1Z28_001331"
     CDS             complement(4865..5362)
                     /locus_tag="J1Z28_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBA7716178.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(5359..5721)
                     /locus_tag="J1Z28_001332"
     CDS             complement(5359..5721)
                     /locus_tag="J1Z28_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBA7716179.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHNTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(5711..6058)
                     /locus_tag="J1Z28_001333"
     CDS             complement(5711..6058)
                     /locus_tag="J1Z28_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBA7716180.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLFIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(6118..7611)
                     /locus_tag="J1Z28_001334"
     CDS             complement(6118..7611)
                     /locus_tag="J1Z28_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA7716181.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVVSYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(7608..9323)
                     /locus_tag="J1Z28_001335"
     CDS             complement(7608..9323)
                     /locus_tag="J1Z28_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBA7716182.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPIFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            9490..10356
                     /locus_tag="J1Z28_001336"
     CDS             9490..10356
                     /locus_tag="J1Z28_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7716183.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEVIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKRQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(10446..12107)
                     /locus_tag="J1Z28_001337"
     CDS             complement(10446..12107)
                     /locus_tag="J1Z28_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBA7716184.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(12304..12732)
                     /gene="ibpB"
                     /locus_tag="J1Z28_001338"
     CDS             complement(12304..12732)
                     /gene="ibpB"
                     /locus_tag="J1Z28_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBA7716185.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(12844..13257)
                     /gene="ibpA"
                     /locus_tag="J1Z28_001339"
     CDS             complement(12844..13257)
                     /gene="ibpA"
                     /locus_tag="J1Z28_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBA7716186.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            13563..13895
                     /locus_tag="J1Z28_001340"
     CDS             13563..13895
                     /locus_tag="J1Z28_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBA7716187.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQETNNVI
                     PPAKMPAN"
     gene            complement(13899..15146)
                     /locus_tag="J1Z28_001341"
     CDS             complement(13899..15146)
                     /locus_tag="J1Z28_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418144.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBA7716188.1"
                     /translation="MAGTVLYQDRAMKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPS
                     GASFTGETIERVNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYY
                     DFHHRRGVIVESGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMH
                     ELDPALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYE
                     EGWVGNHALAFIGDTLSAKGEKVPELFIVELPKDEAGWKAAGDVPLSGTETTLPAPPR
                     GVVQRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEP
                     RQLTHNKTDIQSAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVV
                     FSPDGQWLAWMEDGQLWITETDR"
     gene            15214..16314
                     /gene="cbrA"
                     /locus_tag="J1Z28_001342"
     CDS             15214..16314
                     /gene="cbrA"
                     /locus_tag="J1Z28_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBA7716189.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQYGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYRRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(16275..17612)
                     /locus_tag="J1Z28_001343"
     CDS             complement(16275..17612)
                     /locus_tag="J1Z28_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7716190.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17687..18835)
                     /gene="dgoD"
                     /locus_tag="J1Z28_001344"
     CDS             complement(17687..18835)
                     /gene="dgoD"
                     /locus_tag="J1Z28_001344"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HBA7716191.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(18832..19449)
                     /locus_tag="J1Z28_001345"
     CDS             complement(18832..19449)
                     /locus_tag="J1Z28_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBA7716192.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDVLARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALDAGAQVLKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(19433..20311)
                     /locus_tag="J1Z28_001346"
     CDS             complement(19433..20311)
                     /locus_tag="J1Z28_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBA7716193.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGGNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLVGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(20308..20997)
                     /gene="dgoR"
                     /locus_tag="J1Z28_001347"
     CDS             complement(20308..20997)
                     /gene="dgoR"
                     /locus_tag="J1Z28_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBA7716194.1"
                     /translation="MTLNKTDRIVITLGRQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            21276..21932
                     /locus_tag="J1Z28_001348"
     CDS             21276..21932
                     /locus_tag="J1Z28_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716195.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASRPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVKMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPHGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(21976..22788)
                     /gene="yidA"
                     /locus_tag="J1Z28_001349"
     CDS             complement(21976..22788)
                     /gene="yidA"
                     /locus_tag="J1Z28_001349"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBA7716196.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(22903..23310)
                     /locus_tag="J1Z28_001350"
     CDS             complement(22903..23310)
                     /locus_tag="J1Z28_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBA7716197.1"
                     /translation="MIIMGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQS
                     GGLGAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPK
                     IIDALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(23541..25955)
                     /gene="gyrB"
                     /locus_tag="J1Z28_001351"
     CDS             complement(23541..25955)
                     /gene="gyrB"
                     /locus_tag="J1Z28_001351"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBA7716198.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHNIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNADQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(25984..27057)
                     /gene="recF"
                     /locus_tag="J1Z28_001352"
     CDS             complement(25984..27057)
                     /gene="recF"
                     /locus_tag="J1Z28_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBA7716199.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(27057..28157)
                     /gene="dnaN"
                     /locus_tag="J1Z28_001353"
     CDS             complement(27057..28157)
                     /gene="dnaN"
                     /locus_tag="J1Z28_001353"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBA7716200.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(28162..29565)
                     /gene="dnaA"
                     /locus_tag="J1Z28_001354"
     CDS             complement(28162..29565)
                     /gene="dnaA"
                     /locus_tag="J1Z28_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBA7716201.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            29862..29942
                     /locus_tag="J1Z28_001355"
     CDS             29862..29942
                     /locus_tag="J1Z28_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716202.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            30172..30312
                     /gene="rpmH"
                     /locus_tag="J1Z28_001356"
     CDS             30172..30312
                     /gene="rpmH"
                     /locus_tag="J1Z28_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBA7716203.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            30329..30688
                     /gene="rnpA"
                     /locus_tag="J1Z28_001357"
     CDS             30329..30688
                     /gene="rnpA"
                     /locus_tag="J1Z28_001357"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBA7716204.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            30652..30909
                     /gene="yidD"
                     /locus_tag="J1Z28_001358"
     CDS             30652..30909
                     /gene="yidD"
                     /locus_tag="J1Z28_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBA7716205.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            30912..32558
                     /gene="yidC"
                     /locus_tag="J1Z28_001359"
     CDS             30912..32558
                     /gene="yidC"
                     /locus_tag="J1Z28_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBA7716206.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFVYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            32664..34028
                     /gene="mnmE"
                     /locus_tag="J1Z28_001360"
     CDS             32664..34028
                     /gene="mnmE"
                     /locus_tag="J1Z28_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBA7716207.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPS
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         8257 a         9099 c         8615 g         8121 t
ORIGIN      
        1 cctgcgtgat attgtctgta aaaaaggagc agatatgcgc actcaccctc acttaataaa
       61 acaaactatt gatccacgac tcattaatga aataaacaaa agaacacaaa gcaacagtga
      121 gaagttatca aacagtgata aattaacata tattaatgac aactactgtg agataacgcg
      181 aaactacacg tcattttatg gtatgaacat gctgagcatc gttcttttct tacctatcgt
      241 tattttatgt tttgcctctg ttttattctt tatatatgac ataacattta acttcgaacg
      301 atacttagat acgtggagat acagagcaga ctctgagtta atgctacata ttgcttactg
      361 cgcaattttt ttcatgtctt ttgtttttgc tgctattcta aattatttta tctttgctcc
      421 gcgacattac ccaatccgtt ttaatagaaa aacaggaaag gtctatattt gtgattatat
      481 tcttaatgat atgagtcata aatcaactta tactttctgg tggcactatc cattctataa
      541 acataccaag cctgaatata aagagtacga ctgggaacaa attcaaggta tagctttttt
      601 tgcacaaaat cgccattcct catactccac tattcgctgt attgtttatg aatcattaga
      661 cagtacaaat attattgaca cctttaacct gataacgaca gagagtgcta tagatgcatt
      721 gcatatgaca aatcacaaat tatggttatg gattaatagc tttatgaatt ttgacgatga
      781 gtttttagag aaatctatca atactgctcc aggtatttgg ggccgtgaag tgaattggcc
      841 tgaagatatg aataagaaat caatgttgtc ttcaatggac gaatatatac tctgaataat
      901 tcgagttaca gaatatactc gtcatacttc aagttgcatg tgctgcgtct gcgttcgctc
      961 cccccagtca cttacttatg taagctcctg gggattcact cgcttgtcgc cttcctgcaa
     1021 ctcgaattat ttagagtata taagctcgcc cggatgtaaa tatcccatcc aggcgagaaa
     1081 atcacttact gaaaaaacac cgcaatcttg ttaaacatgg tcggatcgga ctgattacgc
     1141 tgcactttca ccacatcttc cagcttgtcg atttggctta tcatctgctc cagacgctgg
     1201 tcgtcattga ccagtagcca gatgtggctt ttgtcgctgt cctgaatcgg caggcagaga
     1261 atgccttcaa cgttaaaagc gcgacgggca aaaaggccac aaacgtgggt cattacaccc
     1321 ggatggttgc gaacggtgag ttccagaatc acgttatcat gagttgtgtt ttgcatggct
     1381 tattccccca ccatttcagt attcgccgca cctggcggca ccatcgggta cactttttct
     1441 tcggcatcaa tgcgcacatg gatcagcgcc gggccagggc gattgatgat ttcctgcaat
     1501 gcagcctgcg gatcggcttc gttattcaaa tcacaggttt cgaggccgaa tccggcggca
     1561 atctgcataa agttgatttt gcccggatag gtagcggcaa aaacgccctg cttgtagaac
     1621 agactctgtt gctgatgcac cagccccagc gcttcgttgt tcatcaaaat gattttaaca
     1681 tccagctgat tttcactggc ggtcgccatc tcctgaatat tcatcatcag gctgccgtcg
     1741 ccggagaagc acaacacttt gcggtccggg ttcgccagcg ccgcgccaat cgccgcaggc
     1801 aggccaaaac ccatcgtgcc cagcccgccg gaggtcagcc actggcgtgg gcgattgagc
     1861 ggataggctt gcgcggtcca catctgatgc tgacccacgt cggtggtgat aatcgcattg
     1921 tcatcgacac aggcggcaac ggcgttgatc aggccgtaat ggcttaatgg atcgcacgct
     1981 ttcgggattg gacacgggaa ttcacgctgc aaatccgcca ccaactggtg ccactctgca
     2041 cgcggttgcg cttccaccag cgggatcaac tgcgccaaca cgtcatcaac atccgcctga
     2101 attgccacat gcggttgctt gattttgccc agctctgcgc ggtcgatatc gacatgaata
     2161 attttggcat tcggacagaa ctgctcggtt ttgccaatcg cccggtcatc aaaacgcgca
     2221 ccgagtacaa tcagcagatc cgcctcatgc aaaatatagt tggtgctgcg cacaccgtgc
     2281 atccccagca tacccagcga cagcggatgc gcttttggca acatacccag cgccattaaa
     2341 gtcatggtgg taggcagttg cgctttctcc gccagttcac gcactcgcgc aggcgcgttg
     2401 atcacaccac cacccagata aagcaccggg cgtttggcgg cgttaatcat cgccgccgcg
     2461 tcacgaatac tttcttcgct aaaggcgggt gcggcggctt tttctgccat agcgggctgt
     2521 gtttcaatct caaaaactgc cgtttgcaca tccttaggaa tgtctatcca caccgggcca
     2581 gggcggcctg attgcgcaat acggaaggca tcgctcatga cctgcgggag ttcttcgata
     2641 tgtctgacca gatagttgtg tttggtgatg gggatagaga tgccgtaggt gtcgacttcc
     2701 tggaaggcgt cggtgccgat catcgaggcg ggaacctgac cagtgatgca aatcagcggg
     2761 atggagtcca gcctcgcatc ggcaatggcg gtcaccaggt tagtcgcacc cggtccgcta
     2821 caggccatac agaccgccgg tttaccgtcg gtgcgcgcca ttccctgagc gataaacccc
     2881 gcgccctgtt catggcgagc cagaatatgg cggatttgcg tgctttggct taaggcatcg
     2941 taaacaggca ggatagaacc gcccggaata cccgtcacaa tcttaatgcc ctgctgttcc
     3001 aggaaatgaa cgataaattc tgcgccggta aagcgcttac gcgtcgatgt tgtgcccgaa
     3061 cttgccatgc tccagtcctt ttcttctggg ccgactttcc gggaggagct taaacgaaaa
     3121 accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt gttgttccag tccctacggc
     3181 gcattgccga cgaccaccac cacacgcacg acgaccactg cggcggatgg cgcagttggt
     3241 agtagttttg cgttgaacat ggaagtagtc attagggacc ttgttggttt tgtgtttaac
     3301 aatatttata caagcacagc tttacagggg agacaatgga aaatttttct tccggataaa
     3361 aatacgtggc agatcacatt ttgaactgaa actcaggcaa taaagcatca tcacctgaaa
     3421 gacggtcaga ttgttgacag agtgcgcctt attcatgtcg aatacagcgt aaacacctta
     3481 ccctaccaac cgcaccatca ctgttcccgg caacatttat tgccgctttt gtttacatat
     3541 tctgccgcta aacaattccc cattcctggc gtatatctgg ctaacattca tcaatgtgat
     3601 agattcctct cccgcattta tgggaatgcg tagtgactta ttctaattat ttttataaaa
     3661 gcatccgtga taatgaaaag gcatagaaac gtcaatttgt tattgatgtt ggtattactc
     3721 gtggccgtcg gtcagatggc gcaaaccatt tatattccag ctattgccga tatggcgcgc
     3781 gatctcaatg tccgtgaagg ggcggtgcag agcgtaatgg gcgcttatct gctgacttac
     3841 ggtgtctcac agctgtttta tggcccgatt tccgaccgcg tgggtcgccg accggtgatc
     3901 ctcgtcggaa tgtccatttt tatgctggca acgctggtcg cggtcacgac ctccagtttg
     3961 acggtattga ttgccgccag cgcgatgcag gggatgggca ccggcgttgg cggcgtaatg
     4021 gcgcgtactt tgccgcgcga tttatatgaa cggacacagt tgcgccatgc taacagcctg
     4081 ttaaacatgg ggattcttgt cagtccgttg ctcgcaccgc taatcggcgg tctgctggat
     4141 acgatgtgga actggcgtgc ctgttatctc tttttgttgg ttctttgtgc cggtgtgact
     4201 ttcagtatgg cccgctggat gccggaaacg cgtccggtcg acgcaccgcg cacgcgcctg
     4261 cttaccagtt ataaaacgct tttcggtaac agcggtttta actgttattt gctgatgctg
     4321 attggcggtc tggccggaat tgccgccttt gaagcctgct ccggcgtgct gatgggcgcg
     4381 gtgttagggc tgagcagtat gacggtcagt attttgttta ttctgccgat tccggcggca
     4441 ttttttggcg catggtttgc cggacgtccc aataagcgct tctcaacgtt gatgtggcag
     4501 tcggttatct gttgcctgct ggctggcttg ctgatgtgga tccccgactg gtttggcgtg
     4561 atgaatgtct ggacgctgct cattcccgcc gcgctgttct ttttcggtgc cgggatgctg
     4621 tttccactgg ctaccagcgg cgcgatggag ccgttcccct tcctggcggg caccgctggc
     4681 gcgctggtcg gcggtctgca aaacattggt tccggcgtgc tggcgtcgct ctctgcgatg
     4741 ttgccgcaaa ccggtcaggg cagcctggga ttgttgatga ccttaatggg attgttgatc
     4801 gtgctgtgct ggctaccgct ggcgacgcgg atgtcgcatc aggggcagcc tgtttaagct
     4861 cacgtcaccg cagcatcgtc atcagctcca tgggagaacg atgctgcttt atcagatcac
     4921 gcatcacccg catatgcggc gcggagtaag aataaaacgc ctgatagccc gcacaaagca
     4981 cgttgtctgg tttatcttcc cgagcgtgcg ggcacagccg atgccagtcg cacgcctggc
     5041 agtcacggcg cggttgcgac ttttcgctgc cacaccattt ttgtaaggtg gtgtcaaaaa
     5101 gttcaatcga aatacgactg atatctgccc gcacccaaca ctcgaacagg gcaattaaaa
     5161 atttccccca cagctctgct gtcaccgaag cggccgtcag atcgccccgg tcatcaaatt
     5221 caagatgggg aataagctgt actgagcgga tgcccagctg tttgaaattg tgataaaaat
     5281 cggcaggttg cagagcatcc ttatactcta caaccagttt atgcctgtcc tcttcagcat
     5341 caatgacctg acttcctgtc atgcgactct ctcaataaat acgataagtt gatgtatatg
     5401 cgttacagca aacagtattg cgagagataa caccgcgagg gagatcaaaa atttgtcacg
     5461 gactacgtga aattgggaaa aatcgctatt cgtgacatcc attaaattgc gattacgggt
     5521 gttgtgccag aggatcagcg ccacaatggc gaggatgcca atcgaaatcc agaataacat
     5581 gcccgcctgg tgccagttgt gtttgatagc caacgccatc aacgcgccgt agcccagcat
     5641 ggtacgaaac cacgccagtg atgtgcgttc cggctgcagc cctgggtcgg caatgcgtct
     5701 ggctttgcgg ctatccggca tacaacacca gtcccatgac gatcactgcc acgaccatca
     5761 atattaaact gatgatgaat aagctgttgg tatagggcag gtcctctttc aggcgcatcg
     5821 ctttttcatt gcgtaaccag cgcaagtagc catacatcgc taacccgccg gaaaacagac
     5881 acaacaacaa cgccagcagt tcgcggatga cgggcgtggc gaaatctggc gcaagttgat
     5941 caagcccaac ccccgccgcc agaaatccta gtgcagtacg gatccacgcc agaaaagtac
     6001 gctcattagc cagcgagaag cggtaatcgg gcgcttctcc gaggcgggaa attttcattg
     6061 ttgctccttg taaactgttc ctcgccgaaa aattaggcca ggaacagcaa taatgcatca
     6121 gaacttctgt tttttctctt ccaccttcac cccttgtgtc ggtaaaccgg tgtcatagtc
     6181 gcgtaccact ggagaataac catcctgtgg gcgtggacga aacgccccca tccagcgcgg
     6241 ttgtgcatct ttgcgccacg gacgcaggtt ccattgataa ctgcggaacg gatcgcgaat
     6301 tttgtccatg taatccaata aggcgtcatg cattttgctg cgaacgtcgg caaaatggat
     6361 atcatcgatc aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact
     6421 ggtgaagagg ttgagcacca gtttaaagtc atccgttacc cagcaacgca ccggaataaa
     6481 accgccaaag ctgtcatgct caatctcgta gcgattaaat tccaccaaca cgccacgtgg
     6541 ttctttcacg gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag
     6601 cgccatcatt gtcggcagta agtcaatatg actgactggc gtatcgacct gtcgccgctc
     6661 cccttgcggc gaacggatga tcagcggaat gcgggtgatg tcgtcataca tcgccgctcc
     6721 tttactgatc agcttatgtg cgcccatcat ttcgccgtga tcggaggtat aaataaccca
     6781 cgtattttca cgttgctctg gcgttaaggc attgatgacc cgtccgattt ggtcatcaac
     6841 aaagtcattg caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga
     6901 tggcatcgcc tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctctgc
     6961 tttctcaccc agttcgtagt aaaaatcagt gtatttctct aaatactcca ccggacaggt
     7021 gaacgggtga tgcggctcat catacgaaac caccatcagg aaaggttcgt cggcgcgcgc
     7081 gggctgttgc agaaaatcca ccgcccgatt gctgatgcgg tgcgcccagg tgaaggtttc
     7141 gtcaatatgg ttcgcctgta aatcctcaac gctgtttagg ccattgcgcc acaggctaat
     7201 ctctttctcc gtcagttcac taagatagtt cgctccatcg aaccagtaat cagcgtccca
     7261 ctcaggcgga cactcgccag tgccgaaata gtcatgtcca tcgagatgcc atttgccgat
     7321 gtagcaggtg tgatagcccg catccttaaa gtagcgcccc atggtggaaa tgtttttgcc
     7381 cggcgcgacg ttgttggtcc acggaccgga ctggttagcg tagataccgg tgaatagtcc
     7441 ggcgcgtgca ggtgtacaaa ccggtgaaca ggtgtaggca gaattaaagc gaataccttc
     7501 tgccgccaga ctatcaatat tttgcgtatt cagcggttta ccactatagc aaccgaccat
     7561 attggtggcc tgggtatcgg tcatgatgaa cagaaaatta gggcgtttca taggctatcc
     7621 ttcgcatcgg gagtaaaggt gacggccggg tcgtgacgat gccgccagct gtcaaaaatc
     7681 aggtagacaa tcactacggc ggcgatgaaa taactgatca tcgctaacca gcgcgtaccg
     7741 tagccaccaa attcagccag cccggcatag acgccaatca tcgcgaacag gataccaatt
     7801 gacgcaatct tgacgttttt ccacggtttc atgtccaccg caaacgcatc tttgaaggtg
     7861 aacggcgtgg cgcgcggttt gataaaaccg atcaccagca tcacgaccac gttgatgcag
     7921 aacgtacagg ccagcacata gaggaaatgg aagtcgaact tcaccagata gttgatggtg
     7981 atgtagctga ttatgccaat ccccatcgcc acttttgccg ccagcgccgg aatgcgcggg
     8041 aagaaaaagc ccataatgat gatggtaacc agcggcacgt tgtaaatacc gttgagctgt
     8101 ttcatccagc tatacagccc ctgcggcgcg ttggcgatcc acggcgcgac catcaccgaa
     8161 acgatggcga taaagaaacc aaatttgcgc ccgacggtga ccagctgttg cggctcggca
     8221 ttctggttaa tgatgcgccg gtaaataccc atactgaata acgtactggc actattcaga
     8281 aagccgttga aggtactgat caccgcacca aataacaccg caccgaagaa gcccaccagt
     8341 ggcaccggca gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt cggtaaatcc
     8401 tgatacagat gaaacgcaat caaccctggc agcaccagta ccagcgggtc gagcattttc
     8461 agcaccgccg ttaacagcgc ccccttttgc ccttccgcca ggctttttga cgccagcgtg
     8521 cgttgcacga tgccctgatt tgtacaccag taaaaggtgt taaccagaat caaaccggta
     8581 aatgccgcgc caatcggcaa gggatcggtc gggccgccga ctgagtttaa tttctcggcg
     8641 tgaacggtgg tgagttgctc aatgccctgc ataaagctgc ccttgcccat cgcgatcagg
     8701 ccaaatatcg gcaccatcaa cccgccaata accagcccaa taccgttaat ggagtccgcc
     8761 actgccattg cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag accaagcaaa
     8821 ataaccagta accagatagc cgcaccatcg gaaagttgta gcgactcacc cacatgaaac
     8881 aggctgttga gcgccaacgc gccggagtag agaacaatcg gcagaaagca gacgccggtg
     8941 gcaatgagga agcagaagtc gatgataata cgcgtcgttt tatcataacg ttcctccaga
     9001 aaatcgggga tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat tagcgcgagg
     9061 aagatcagcg tcactgcaga ggtgacttcc cagcccatca ccgacatgcc gcttttgtag
     9121 gcctgcccgg aaaggccgac cagttgttcc gtggaaaggt tggttagcat taacgaagcg
     9181 gcaataaccg gcgcttttag tgaacgtccg gcaagaaaat agccttgttg cgatccagta
     9241 tctgttttgc ggactttcca ccaggtgatg accgccacca gcagcgtaaa accgacaaaa
     9301 ctcaagattt gtaacgaatt catctcatag cccttattta tgtttatgtc ccggacgcat
     9361 gaatataggg agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa atacaaacgt
     9421 catatccctg aacggattgt tttctgttaa gttcaggttg tgagcataat tcgccggagg
     9481 gatattaaaa tgaatggaaa attgcaaagt tcggatgtaa aaaacgaaac tccgtacaat
     9541 attcccttat tgattaacga aaatgtgatc tccagcggaa tttctctgat atcgctctgg
     9601 catacctacg ccgacgagca ttatcgggtg atctggccgc gggacaagaa gaaaccgctt
     9661 atcgccaact catgggttgc ggtgtatacc gtacaaggat gtgggaaaat tcttttaaag
     9721 aatgacgaac aaataactct gcatggcaac tgtattatat ttttaaagcc aatggatatt
     9781 cactcttatc actgtgaagg tttggtctgg gaacagtatt ggatggaatt tacccccacc
     9841 agtatgatgg atattcccgt cggtcagcaa agcgttattt ataatggcga agtttataat
     9901 caggaactca ccgaagttgc tgagttaatt acttcaccag aagtaataaa aaataatctg
     9961 gcagtcgctt ttctgacgaa aattatttat cagtggattt gtcttatgca cgcagtcggt
    10021 aaaaaagatc cacaacggcg gcaaattgaa aaattaattg ccactttaca tgccagtcta
    10081 caacaacgct ggagcgtagc tgatatggct gccacgatcc cctgtagcga ggcctggttg
    10141 cgtcgtctgt ttttacgcta taccggcaag acgccgaaag aatattacct cgatgcgcgt
    10201 ctggatctgg cgctatcgct attaaagcga caagggaact cggttggcga agtcgctgat
    10261 acgctcaact tcttcgactc ctttcatttc agcaaagcct ttaagcataa atttggttat
    10321 gcgccgtcag ctgtgctgaa gaatacggac aggtaataac cagaatggcg agtgtgtaaa
    10381 tgactcgatg atgttcagaa ataccaagcg gagtaccatt tacggcgact ccacccggaa
    10441 aagagttaac cgatactcca gaaaagcacc gccagtaatt ggggtgtgat aatgcgcagg
    10501 aacatcacta acggatagac agtggcgtaa gagagcgccg ccgcaccgct ggttggatga
    10561 agattattag caaacgccag cgccggagga tcggtcatgg aacctgccag catcccgcac
    10621 atggtcaggt aattcatttt ggctaacatc cgcgccagaa tgccaacagt aatcagcgga
    10681 acggcggtga tcagggcacc ataaccaatc cagcttagcc cttcgccatc caccagtgta
    10741 tggataaaat cgccgccgga tttcaggcca accaccgaga gaaagagcac aatgcctaac
    10801 tcgcgtagcg ccaggttagc gcttggtggc ataaaccagt acagcttgcc gatactgccg
    10861 atacgcccga ggatcaacgc cataatcagc ggaccgcccg ccagcccgag tttcaacgcc
    10921 gccgggaacc caggcacaaa gacgggaata gaacctaaca atacgccaag cccgatacca
    10981 ataaacactg gcagcatctg aacctgttgc agtttttgct gcgcattccc cagcacattg
    11041 gcaacggcat caattgcgga cggacgcccc accaggttga ggatatcgcc gaactgcagg
    11101 ctgatatcgc tacttgcaac taattcaaca ccagcacggt tcaggcgcga gataacaacg
    11161 tcatagcgtt ctttaaagtg caggtcgcga atacgtttac cgagcacgtt ttcattggtg
    11221 accaccacac gcgcgacgcg caaatcagtg cctttcgtag acagcgaggt atcgacctcc
    11281 tgaccaatca ccagttgcgc attatgtaaa tccgcaggct gccccaccag atgcagcaaa
    11341 tcgcccagtt gaataacggt ttctggcgaa ggcactttta gggtttcttc tcgtttcaat
    11401 cgtgagcaaa taactttatc gccattgaga atcggcacat ctttaatggc taaattatgc
    11461 aaattaggat tctcaactcg gatgttaatc gtcctgatta gcgcaccgcc gttggttcgc
    11521 gtggactcgt gctgctgggc ctcagtctca acattgacgc ggaaaataac ccgcagcatc
    11581 cacatagtga acaaaatgcc gcaaatccca aatggatacg ccatcgcata gctcatcccc
    11641 atttgatcga ccatcgccat cggtgtcccc aggtcgcgca agatctgctg ccctgccccc
    11701 agcgctggcg tattggtgac cgcgccggaa aaaatcccca gcactaccgg cagtggaata
    11761 tcaaacagtt tatgcaggat ggcggtaacc agaccaccga tgatgacgat cagaacagca
    11821 aacaggttga ggcgtaatcc ggagacgcgc aatgaggcaa agaagcccgg ccctacctga
    11881 atcccgatgg tataaacgaa caggatcagg ccaaactcct gaataacatg cagcatatcg
    11941 ctacttaatg tcatccccgc ctgagaaaca aaatggccga cgatgatccc accgaacagc
    12001 acgccgccaa tgcccaatcc aacaccgcga aacttgacgt tgccgataaa caacccgacg
    12061 accgccacca aagccagaat actgaccgtt aatgctatat cactcatcgt ccatttctct
    12121 tgaaataaca ttttaattgc tacggattat ggctgaatcg gtgaagcaga tttgccttat
    12181 agcgcacatt atcacgggaa agaacgtgcc gaaatatctt aaacaggcga aatttaacgc
    12241 attgctgaag aaaaaagaca aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
    12301 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
    12361 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaggttg cgccagagac
    12421 ttccatattt tcagccagcg taaagctcag gctaaaaggc tgattcataa gcccttgatg
    12481 cagccatttt ttctcttctt ttggctgctc tggcgtgcct tttacgctca ggcgcgtacc
    12541 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
    12601 gtggttatcg tcgcttttct caatattgta cggcgggaag ctctggcttt caccggcgtt
    12661 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
    12721 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
    12781 ttcgcatatg ctccctgacg gcgagcatgg aaatgtcagg ccgcgccagg cggccttagg
    12841 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
    12901 gatatacagc agaccattca ccaggttagc accacgaaca tgaatgttct cagctaactg
    12961 gaatttacgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
    13021 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
    13081 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
    13141 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
    13201 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
    13261 caatagctcc tgaaatcagc gagaatctaa gaccttccac aatggacagg tcaggtagcc
    13321 agaacaccca tcaggcgcgt cctcatcggc tacgatgtaa aaatgggtct ggaaatgaac
    13381 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
    13441 gggcagcacg aaacgttaaa gtgcgatagc cgccacagtg cgacgtaatg atgatgttat
    13501 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
    13561 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatgaca ttactcggag
    13621 gatgctccag cgtgatgtcc cataccgggg ggaaagaagg aacgtatcct ggcacccgcg
    13681 ccagcgcaac catgattggc gacgatgaaa ccaactgggg cacaaaatcg ctggcgatac
    13741 tcgacatgcc atttaccgca gtactggata caatattgct accgtgggat gtgttccgca
    13801 aagacagctc agtgcgctcg cgcgttgaaa aaagcgaagc caacgcgcaa gaaaccaata
    13861 acgtcattcc ccccgccaaa atgcctgcaa actgagagct agcggtcagt ttcggtgatc
    13921 cacaactgac cgtcctccat ccacgccaac cattgaccat ccggcgagaa aaccacggcg
    13981 tccgcagaag gtggattggc gtggttttcg gttaaatact caacctcgcc gctttgcgca
    14041 tgggcacaag caatccgatt atccagcaca aatcccagcc attcgcctga cggatgccag
    14101 ttaaatgcag actgaatatc cgttttgtta tgggttaact ggcgcggctc gccgccctgt
    14161 ggcgagataa gccacagttg cacaatgccg ttatcatcac gcattaaaaa cgcgatttgc
    14221 gtaccctgcg gattacagcg cacccagtgg cgcgggacgt tgactaatcc cggataagcc
    14281 cgatggtgcg taaaggttaa acgtcgctgc acgacaccac gcggtggcgc aggcagggtt
    14341 gtttccgttc cacttaacgg cacatcacct gccgctttcc agccagcttc atctttcggc
    14401 aactcgacaa taaacagctc cggcactttc tcgccctttg ccgaaagtgt gtcgccaata
    14461 aacgccagcg cgtgatttcc tacccatcct tcttcataag cacgattgat ttcatcactg
    14521 ccaggctgtg gcgtgggcgt ggttttactt accagcacgc accagtggct accgctgtat
    14581 tcacgcggat gctgcttttg tacgttgacc gggccaaacg gtgcagcaac accgacgttt
    14641 cgcaaatcca gcgccggatc gagttcatgc attacatggt cgttgtaggt aaagctcacc
    14701 ctttcaccgt tcgggctaaa gacatgtaca tggctgccgc cacgtagcgc tcctggcgta
    14761 tatggcgcgg taatatccat tgcatcgaga ttgctcacct tgccgctttc aacaatcact
    14821 ccgcgccgat gatgaaagtc gtaataccat gtttcatcag gattttccgg gccgtggatg
    14881 aagacatact tctctgactt cggatgaacg gtcaccacgc cgacgtatgc gccctgtgac
    14941 gcgcgataga taacctcgac ctcgccggta tggatattca cacgctcaat ggtctcgccg
    15001 gtaaacgacg ccccagaagg ccgcacgtca aacaccagcc actggctgtc gggcgtccag
    15061 gtattggtat tggtgagcag gtgattacga ggagcaaagg ttatctgttt catggcgcgg
    15121 tcctgataca aaacggttcc cgccataata cttcacaagg acttcacttt cagacaatag
    15181 tcttttgcca tcgtgatgaa tgtgagtgaa aagatggaac atttcgacgt ggcgattatt
    15241 ggcctcggcc cggcagggtc ggcgttggcg cgaaagttag caggcaaaat gcaggtgatc
    15301 gcactggata aaaagcacca gtatggtaca gaaggtttca gcaaaccttg tggcggtctg
    15361 ctggcaccgg acgcgcagcg atcttttatt cgcgatggac tgacgcttcc tgtcgatgtc
    15421 atcgccaacc cgcagatttt cagcgtcaaa accgtcgacg tcgccgcatc gctcacgcgt
    15481 aactaccagc gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg
    15541 ctgatccccg ccagcgttga ggtttaccac gacagcctgt gtcgaaaaat ctggcgtgag
    15601 gatgataaat ggcatgtcat ttttcgcgca gacggctggg agcagcatat taccgcccgc
    15661 tatctggtcg gtgccgatgg tgccaactcg atggtgcggc ggcatctcta cccggatcat
    15721 cagattcgta aatatgtcgc catccagcag tggttcgcag agaaacatcc ggtgccgttc
    15781 tactcctgca tctttgataa tgcgataact aactgttact catggagtat cagcaaagac
    15841 ggttatttta tctttggcgg tgcctatccg atgaaagacg gtcagacgcg tttcacgacg
    15901 ctaaaagaga aaatgagtgc ctttcagttc cagtttggta aggcggtgaa aagcgaaaaa
    15961 tgcacggtgc tgtttccctc acgctggcag gattttgtct gcggcaagga caacgccttt
    16021 ctgattggcg aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg
    16081 ctggatagcg cagagattct gcgttcggta ttactgaagc agccagagaa gctcaatacc
    16141 gcttacaggc gcgccactcg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc
    16201 cgatgcctga ccgcaccggc tttaagaaag tggattatgc gcagtggagt ggcgcatatt
    16261 ccacagttga aagattatcc aacgcgcttc acatcgccca ccagcaggat gtaagagagc
    16321 gcgccaatca acgcgacggc ggagatataa accagtgcag gtgcgaaacc gtaaccctgc
    16381 gccaggtacc ccaccaccag cggaacggtg atgccgccca gaccaccggc aaagttaaac
    16441 acgccgccgg ttaaaccaat caggcgcatc ggtgccagag aagagaccaa cgaccaggta
    16501 atcgaagcaa aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga
    16561 tcgttggtgt agttcgcacc cataatgcag gtggagatca gcaagccgca gataatcggc
    16621 gttttacgcg caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcct
    16681 gagagcagga cgccaataaa cgccgcaagg aacggcactg tggtcataaa gcccgctttc
    16741 agcgccgtga ttcctttttc ctgagttaaa tagttcggga accaggttaa gaagaaccac
    16801 agtgtagaag ccaccgcaaa ttgcccaaga tagacaccga tcagtttacg atggaacacc
    16861 agtttccagt ctttggctgt taacggctga cgcgcctctt ttttcaccgg cgcatcacca
    16921 tccaccagac cgccgccatc acgaatgtaa tccagttcag ctttgctgat gcctttggtc
    16981 agacgcggcg gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccaccg
    17041 gtgacaatga acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc
    17101 agaaacgcca gaccgacaaa ctgaccagac gtataaaaac caacggcaga agcacgttca
    17161 tgttccggga accaactggt taccatccgg ttattggtcg ggaacgcggg cgcttcgaaa
    17221 ataccggtta tcgcgcgcag accaattaat gacattaagc ccgtggcaaa gccctggaat
    17281 aacgtcgcca ctgaccagcc aaatatcgca ataaaataag tcacgcgtga acctacgcga
    17341 tctaaaaacc aaccaccggg gatctgacat agcgtataaa gccaggcgaa ggccgaaaat
    17401 acatagccca tttgcgcttt agtaatacca aactcttcct gaatatgggc agaagccacg
    17461 gccaggttag cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc
    17521 agataacgcc gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc
    17581 cagattttgg gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt
    17641 ttgtattgct gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta
    17701 cgctgttatc ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact
    17761 caataacttt agcttcgtcg atttccacgc ctaagcccgg tttcgttagc ggtttaaaga
    17821 agccgccgac catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt
    17881 tgttgtaatg aatgcccata ctttgttcct gaagtaccgc gttataggaa acaaagtcga
    17941 tatgcaggca agccgccagt gcaatcgggc cgagcggaca gtgcggcgca agggtcacgt
    18001 cataggcttc cgccattccg gcaattttgt agcattcggt aataccacct gcgtgagaaa
    18061 gatcgggttg cagaatcgaa ataccgccag cttccagcac gcgtttaaaa tcgaagcgtg
    18121 agaacatgcg ctcacccgcc gccagtggaa tatgcgtttg tgccgccagt ttcgggtagt
    18181 attcggcttg ttccgccagc accggctctt caataaacag agggcgatac ggttccagct
    18241 ctttaatcag cactttcgcc atcggcgcgc tgacgcgacc atggaaatca agaccaaact
    18301 caatctgatt gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg
    18361 cgcgggagtt atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc
    18421 cgatttcgcg cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc
    18481 aactgtaggc tttaattttg tcgcgaacca ggccgcccat cagttgccac accggcgcat
    18541 tcagcacttt gcctttgata tcccataacg cctggtcgat accggcgatg gcactcatca
    18601 gaatcggacc gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc
    18661 gtgacgggtc ctgaccaatc aaatagtcac tcagctcgtg gactgccgct tccaccgtac
    18721 gggcgcggcc ttcaatcacg ggctcgcccc agccgaccac gccttcatcg gtttcaattt
    18781 tcaggaacat ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac
    18841 tgcctctcga tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg
    18901 cccggcgcga tagagatcgc tgcctaagcc tgcccctgca caacctgcgt ctatccactg
    18961 cgctaggttt tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac
    19021 cgcttttaac gctttgatgt attgcggacc aaaagccgat gaagggaata ttttcagtac
    19081 ctgcgcaccc gcgtcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac
    19141 ggtcatgccg tagcccaccg cacggcggat cacttcacta tggatattgg gcgtaacgat
    19201 gagctggcag cccatcctgg cgagcacatc gacctgttca ggtttcagta ccgtacctgc
    19261 gccaatcaac gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg
    19321 tggggaattc agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg
    19381 cgccagcgcc tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg
    19441 ccactgcatg agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gctaccgccg
    19501 tcacgtcaca acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg
    19561 cgacaagggt gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc
    19621 caatcaacaa accagagaga aattcgctga cctgttcgcg gggaagcgtt cccagcacat
    19681 gcgaggcgcg aacttcaaaa agctgcggca atatggcggg cgcattaagg ccacgctcaa
    19741 ggccagccgc gaaggcatcg gcagagtttt cctgcggcgg caaacctgcg ccaaccaatg
    19801 agtgatttaa cagtaaatga tgtaattcac cggtcattac ggtgcgaaaa tcgttgattt
    19861 gctggctatc ggcctgcacc catttgcaat gggttccggg cataacataa agagaggaag
    19921 gagccaaagt gcgcgcgccg atcaattgtg tttcttcgcc gcgcatcaca ttgtggttat
    19981 cgtcatgaga gacgcataat ccgggaataa tccagatatt gccgccaacc gacgttaatt
    20041 gatcgccaat agacgaaaaa cgggcaggga cagataaata cggtgcaact ttccagccga
    20101 cattgctgcc gaccattcct gccattacca ctggcgtatt ctcttcacgc cagtcggtcg
    20161 tcacttctgc taacaccgca gccggagatt ttccgttcag gcgcgtgacg cctgcttctg
    20221 attgcctgct gtccaggcag tggtcgccct gataaagcca ggcgcgcaga ttggtcgatc
    20281 cccagtcaat tgcgatgtag cgagctgtca tgtgatttcc tttaaccttc gtgtcgagct
    20341 ggcgatcatg gtaagcgccg cctgctctgc cgcatcgccg tcctgatgcc ttatcgcatc
    20401 gaacagcgcc ttatgttcct ggagcgtttg cggcatgttg gcctcatcgc ccatccaggt
    20461 tcgttcaaaa accgcccgct gcagcgaact gatcgcaatg ctcagttgct gtaacaccgg
    20521 gttatgcacc gactgcagca ccgcctcgtg gtagcgaata tccgcttcgt taaacgcttc
    20581 gcggtcctga ttgttagcaa tcatctcgtt cagcgccgat tcaatctgcg ccagatcgct
    20641 ggaggtcgcg cgttctgctg cccaacgggc aatcgccggt tccaccagat ttcgcacttc
    20701 gctcattgca ctgataagcc gtgggtcgta gtcgttttcc agcacccatt gcagtacgtc
    20761 agtgtcgagg taattccact ggttacgcgg agccacaaac gccccgcgat aacgtttcat
    20821 ttcaatcagc cgcttcgcca tcagcgaacg aaatacctca cggatgatgt tgcgcgaggt
    20881 tgcaaactcc tcacagagtt ctgcctcagc cggaagcggc gagcctggca cgtatttgcc
    20941 gtgaacgatc tgtctaccca gcgtaataac gatgcgatcg gttttattga gagtcatgga
    21001 gagtccttgt gctctgtatg ttcttctcta ctttaccccg atcgatgcat aacgcggcaa
    21061 ttttgtagta ccagcgtgat gacgttcgcg tttgctgtgc gcctaatgta gtacaaagtt
    21121 atattgttgt actacaattt agatcacaaa aagaacaatc cataagaaaa tgttctacgt
    21181 cgggcaaaaa tctgaaaagg gagatcaggc gctaaactgg ggggaaagaa gagtatgctt
    21241 tcaacggctt agtactcttg tttaaaggat taatcatgaa gttgaatctt aagggatttt
    21301 tcaaggctgc cagtttgtgt tctttggcat ttgcgttgac cggatgtatc acctggggat
    21361 tggtttccaa tactgcaagc cgtccacatg ccgagcaatg gcggtcagac acgattaagg
    21421 gtttatcgct ggcagaagat agtaacggta cgaaaggcta tgtttttgtt ggtgaatcgt
    21481 tggactatct gttgaccacc ggcggcgatg aggtggttaa aatgctgaac gatccggcaa
    21541 tccatggtga aagaatcacc gtttctgata acgcgaaatt tattttaagc agcagtaata
    21601 agaatttctc tggtgctatt acgctctatt acgactggaa caacgaagaa gataaggcac
    21661 tggcaacaca atatggtttt atttgtgata cccggcgttg tacatggatg ctggacggtt
    21721 tgaaaggctc aatccaccag aaaaataaaa aagcggatta ctcaaacgtg atggttttcc
    21781 atcaaccctt tacggtggga ttttatgagt acaaagcaac agatggcgtc ccgcacggcc
    21841 tcgttaatgc gctgctgcca gtcactctta cgctggatat agtaacgtca ccactccagt
    21901 tcctgatttt atgcacgacc agaaattgtt aatcgctgcg ggcaaacgca ttcatgccgt
    21961 ttgcccgttc actgattaat tcagcacata cttctcaata gcaaatgcca cgccatcttc
    22021 aaggttagat ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat
    22081 cgccacaccg acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc
    22141 catgatttct tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt
    22201 gttaacgcgt ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtgtattt
    22261 ttctttcact tcctgcggaa tacgcgcaat ggcctggtcg aggatggcgg gttcatcaat
    22321 catcatcact ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag
    22381 cggaatggtg gcaacgaaag attcatgcac cgtgtagtag ctgatatcac gattggcggt
    22441 gtacagcgtg gtgcggtcca gcgcgtggaa atgagaaccg acttcacggg agagtttttc
    22501 caggaaacgg tagtcgtcat agctgagagc ggtttgcgcc acagtgctac catcggcggc
    22561 cttctgtacc agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatgtgcag
    22621 ctctttcagg taattatgaa cacctgcata agggcggccc gtcgtcagca cgacatttac
    22681 gccacgggcg cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg
    22741 cagcagaagg gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg
    22801 tagattggtt cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacaccctgg
    22861 ggaaaagggg gatgcaacgc gctttgcgtg ctccccattt gcttagcgga agagtttccc
    22921 tttcagcagt tccatgcctg cggaaagcag atcgttattg gcttgtggtg acacttcacc
    22981 ttgcggtgag agtgcatcaa taatcttcgg caattgttct gccagtaaac tggaagctgt
    23041 actggtatca acgcccagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga
    23101 ttccagttgc tcgccgctaa ccgattgatt gccctgttga ttactcagcc aggttgagag
    23161 aatggcccct aagccgccac tttgcagttt ttccagcagc acctgaatac cgccctgctc
    23221 ctcaacccag ctcaaaatag cctgatattt ccccgcatcg cctttcagaa aggcgccgac
    23281 aacttcatca aaaaggccca tgataatcac ctgtaaagcg ttacgtgttg actcaaaaag
    23341 tatagttctg tgggatgata attgcggatt gcaggaataa aaagggcgga gatgatctcc
    23401 acccttttct tatagcttct tgccggatgc ggcgttaacg tctgatccgg cctacaaaat
    23461 catgcaaatt caatatattg caggagtctc gtaggcctga taagcgtagt gcatcaggca
    23521 cgctcgcatt gttaacgcca ttaaatatcg atattcgccg ctttcagagc gttctcttca
    23581 ataaacgcac ggcgcggttc aacggcgtcg cccataagcg tagtgaacaa ctggtcggca
    23641 gcaatcgcat ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt
    23701 tcccacagct gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg
    23761 cgacgggact ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga
    23821 cgttcgccac gttcgataaa tgcatcttct tccagcaagc cacgcagttt ctcacccagc
    23881 gtgcagatac gacgatattc gccgccagtg ataaattcgt gatccagcgg atagtcagta
    23941 tccacaccgt gggtacgcac gcgaacaatc ggttcaaaca ggttttgatc agcattggtg
    24001 tggacatcaa acttccactg gctgccgtgc tgttctttat cgttcagttc actgaccagc
    24061 gcgttcaccc agcgggtaac ggtttgctcg tcagaaaggt cggtttccgt cagcgtcggc
    24121 tgatagataa gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt
    24181 ttctgcgtcg cgttgtactc agacaccagt ttctctaacg cttcgccagc cagcgccggt
    24241 gcactggcgt tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc
    24301 atcgcttcgt cgtctttaat gtactgttcc tgcttgcctt tcttcacttt gtacagcggc
    24361 ggctgagcga tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaagaag
    24421 gtcaacagca gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg
    24481 ttgtgataac gcagtttgtc cgggttgtac tcgtcacgac cgataccaca accaagcgcg
    24541 gtgataagcg tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg
    24601 ttgaggattt tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc
    24661 gcagagccgc ccgcggagtc cccttccacc aggtacagtt cagagagcgc cggatcgcgt
    24721 tcctggcagt ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg
    24781 gtcatttcac gcgcgcgacg cgcagcttca cgggcacggg cagcatcgat aattttgcca
    24841 accacgattt tcgcgtcggt tgggttttcc agcaggtatt ccgccagcag ttcgttcatc
    24901 tgctgttcaa ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgtgaggag
    24961 aatttcgggt ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca
    25021 ccagtggcgc tgactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcaga
    25081 gtacgggtca tcgccgcacg gaaacctgcc aggtgagtac cgccgtcacg ctgcggaatg
    25141 ttgttggtaa agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg
    25201 acgccaatac cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg
    25261 ttcttgttca gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct
    25321 ttgccgtcgc gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca
    25381 cgcagacgtt tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg
    25441 ctgggccaga aacgcaccat ggtgcctgtt ttttcagtct cgccggtaac cgccagcgga
    25501 gcctgcggta caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc
    25561 agctccagtt tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg
    25621 gacactttat aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact
    25681 tccgccgccg atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc
    25741 tgtacagaga cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg
    25801 agcgcttcgt cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg
    25861 tcatccgtgt cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg
    25921 actttgatac tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt
    25981 gggttaatct gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat
    26041 gtctataacg tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa
    26101 gcggctggca agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag
    26161 gtagagacac cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc
    26221 gcacatcagc agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc
    26281 gcgaatgcgt aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg
    26341 ttcaaaatta cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa
    26401 agagaaagtc agagaaaact cagggagaaa ttgcttacag gtatcggcca tatcagccgc
    26461 gataccggcg ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagttc
    26521 tttatcccac ggacgtagct gttcgtaacg agtcacctgg cgcagcgccg cattgcgctg
    26581 cttgagcaat cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca
    26641 tccccagtcg aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc
    26701 tggcgttatc agctgcattg gcatcaggtg cgccagctcc gcgactttat gcccgtctgt
    26761 accgtcaata cggactttgc tatcgccttg tttgtctttg gttaaaccaa tcgccgtctc
    26821 gcgctcttcg ccctgtaatc gcccgtggag aacaaacgcc tcctgctcgt ggcgaatgac
    26881 gcgaccaatc tgcaaactgc gaaacgcccg accgtggccg agcgtataga tggcttccag
    26941 cacgctggtt ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaggc
    27001 gagatccgcg gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac
    27061 agtctcattg gcatgacaac ataagccgcg ctctggctgg ccgcatcttc aatctgcacg
    27121 ctggaaaccg aatcggtcag catcatgcgg acgttttcgc atttcagcgc gttgagaaca
    27181 tccagcacat agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg
    27241 aggatctctt ccgcttcttc ctgttctggg ttgttggcgg tgattttcag ctggttttcg
    27301 ctgacataca gacgcacgcc gcggaatttc tcgttagaga gaattgccgc acgggcaaac
    27361 gcctgcttga gcagatcaca gccagcttcc agatgcttgt ccgggttctt cggcagaacg
    27421 cgacggtaat ccgggaagcg accatccacc agtttcgagg tgaagataaa gtcgccaacg
    27481 tgggcgcgaa tgttgttgct gccaatctgc acgcgcagcg gattgtcgcc gccgtcgagc
    27541 atacgcatca gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat
    27601 tgaccaattg gcattgaaca gaccgccaga cggtggccgt cggtcgccac ggtgcgcagt
    27661 tcttcacctt cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc
    27721 atagaaaact gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg
    27781 acttcgctct gccagtcatc gaggttcggg aaatctgccg ctggcagggt agacagcgaa
    27841 aaacggctac gcccggagcg caccagcatc cgctcacctt ccagctgcac ggcaatttcc
    27901 gcgccttcag gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgcc
    27961 cctggttcgt gcggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta
    28021 ccggtcagcg acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc
    28081 gtaggacgac cacctaacgg accgctcacc tgttgtagcg gttttaataa atgctcacgt
    28141 tctacggtaa atttcatagg tttacgatga caatgttctg attaaattgg aaaaatcttc
    28201 tttgatatcg tggctctctt cacgcagctg ctcgatctta cggcaggcat gaagcaccgt
    28261 cgtgtggtca cggccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagttc
    28321 tttcgccagc gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga
    28381 aaggagatcc gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat
    28441 ggtgaccagt ttttcctgca atgccagcaa gtcgcgcagc gcctcacgca cgaagtcgat
    28501 ggtgatcgca cgtccggtaa agttggcgtt agcaataacg cggttcagcg cgccttccag
    28561 ctcacgtacg ttagatcgta gacgcttggc gataaagaac gccacttcgc ccggcaaacg
    28621 aatgtcgttt tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg
    28681 ttcgatcgcc acagtcagtc cccagccgaa gcgggatttc aaacgatcct caacgccgtt
    28741 gatctctttc ggatagcgat cggaggtgag aatgatctgt tgattacctt ccagcagggc
    28801 gttgaaggtg tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc
    28861 gtcgatcagc agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt
    28921 ttgcagagct ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc
    28981 attcggcttg cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccaa
    29041 acccgtgccg ccataaagga acaacgggtt ataagcaccg ccagggttat ctgccacctg
    29101 gcgagccgcc gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg
    29161 tttgacgttt acgttagaac gataggtcgg ttctgccgga gccgggacgt tatcccaacc
    29221 cgagcgcgta gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc
    29281 gacgttgctc gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa
    29341 acgcagctgt ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag
    29401 gtacttgtcc cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt
    29461 atcgctcagt tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc
    29521 atcctgcaat cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg
    29581 aacaaaagtc gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag
    29641 accctgcatg agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct
    29701 gcaaaacgat cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca
    29761 cagattttcc cgatttatcc acaggatgat ccagaactcg ctaagtgtaa acgatcctgc
    29821 cgcgggccgg gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt
    29881 ggcttaatcg atctaataaa gatccaggac gatccttgcg ctttacccgt cagcccgtat
    29941 aatcctccac ccggcgcgcc atgctggttt tcactggtgt gaggccgtac gttttccccg
    30001 cgaaaaggtg cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat
    30061 tattacaatc cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg
    30121 cggatatcca ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc
    30181 acttttcaac cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct
    30241 actaaaaatg gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc
    30301 gtttctaagt aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg
    30361 cttgttaact cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc
    30421 gcaaattacc attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt
    30481 cgccaagaaa aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga
    30541 aagcttccgt ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa
    30601 aggggttgcc gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg
    30661 ccactgtcgc ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt
    30721 agtccgctac tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag
    30781 gcattgcgca ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa
    30841 tgccaccctt tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga
    30901 gaacactaac gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt
    30961 tcatgatctg gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca
    31021 cgcagacaac gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc
    31081 aggggaaact gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg
    31141 gtgatgttga gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt
    31201 tccagctgct ggaaacttca ccgcagtttg tttatcaggc acagagcggt ctgaccggtc
    31261 gtgatggccc ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg
    31321 cttatgtgct ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg
    31381 caggcaacac gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca
    31441 actacaacgt gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga
    31501 agcaatccat cactctgcca ccgcatctcg atactggaag cagcaacttc gcactgcaca
    31561 ccttccgtgg tgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata
    31621 ccattgccga taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc
    31681 aacagtattt cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg
    31741 ctaatctggg taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc
    31801 ctggtcagac tggcgcgatg aacagcaccc tgtgggttgg cccggaaatt caggacaaaa
    31861 tggcagctgt tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct
    31921 ctcagccact gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct
    31981 ccattatcat catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt
    32041 acacctccat ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc
    32101 tgggcgatga caaacagcgc atcagccagg aaatgatggc gctgtacaaa gctgagaagg
    32161 ttaacccgct gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt
    32221 actacatgct gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg
    32281 acctgtctgc acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct
    32341 tcattcagaa gatgtcgccg accactgtga ccgacccgat gcagcagaag atcatgacct
    32401 ttatgccggt catcttcact gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact
    32461 atatcgtcag caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa
    32521 aacgtggcct gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat
    32581 aagggcggtc agttgaccgc cttttttctt ttcgtagggc ggataagcac cgcacatccg
    32641 ccacacaaag caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc
    32701 ctccgggacg tggcggcgtt ggcatcctgc gcatctccgg cctcaaagcc cgtgaagttg
    32761 ccgaaaccgt gctgggtaaa ctgcctaagc cacgctacgc cgattatctt ccgtttaaag
    32821 acgccgatgg cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt
    32881 tcaccggcga agatgtgctg gaactgcaag gtcatggcgg tccggtgatc ctcgacctgc
    32941 tgttaaaacg cattctgacc attcccagcc tgcggattgc ccgccctggt gagttttccg
    33001 aacgcgcatt tctgaacgat aaactcgact tagcccaggc cgaggcgatt gcggatctta
    33061 tcgacgccag ctcggaacag gcggcccgtt cggcgctaaa ctcgctgcaa ggcgcattct
    33121 ccgcacgggt caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg
    33181 caattgattt cccggatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc
    33241 tcaatgacgt aattgccgat ctcgacgcgg tacgcgccga agctcgtcag ggtagtttgt
    33301 tgcgcgaagg gatgaaagtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgc
    33361 tgaacgcgct ggcgggccgt gaagcagcaa tcgtaaccga tatcgccgga accacgcgtg
    33421 acgtgctgcg tgagcatatc cacattgacg gaatgccgtt gcatatcatc gataccgccg
    33481 ggctacgtga agccagtgac gaagttgaac gtattggtat cgagcgcgca tggcaggaaa
    33541 ttgaacaggc agaccgcgtg ctgtttatgg tcgatggcac cacgacagac gccgttgatc
    33601 cggcagagat ctggccggaa tttatcgccc gtctgccagc gaaactgccg atcaccgtgg
    33661 tgcgcaataa agccgatatc accggcgaaa cgttggggat gagcgaagtg aacggtcacg
    33721 cgttaattcg tctttcggcg cggactggcg aaggcgtgga tgtgctgcgt aaccatctca
    33781 aacagagcat gggctttgac accaatatgg aaggcggctt cctggcgcgt cgtcgtcacc
    33841 tacaggcgct ggaacaggcg gcagagcatt tgcagcaagg taaagcgcaa ctgttaggtg
    33901 cctgggcggg tgaactgctg gcggaagagt tgcgcctggc gcagcagaac ttaagcgaga
    33961 tcaccgggga atttacttca gacgacctac tggggcggat tttctccagc ttctgtatcg
    34021 gtaagtaacc gcgcttgcga agccgcattc tgactgtcag atgcggcttc gcttcattgt
    34081 tagcactcct gt
//
LOCUS       DADSZD010000031        33783 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.031, whole genome shotgun sequence.
ACCESSION   DADSZD010000031 DADSZD010000000
VERSION     DADSZD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.031"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            112..396
                     /gene="pduA"
                     /locus_tag="J1Z28_001361"
     CDS             112..396
                     /gene="pduA"
                     /locus_tag="J1Z28_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBA7716208.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAARNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            393..1205
                     /gene="pduB"
                     /locus_tag="J1Z28_001362"
     CDS             393..1205
                     /gene="pduB"
                     /locus_tag="J1Z28_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBA7716209.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQGTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDRPSYI"
     gene            1224..2888
                     /gene="pduC"
                     /locus_tag="J1Z28_001363"
     CDS             1224..2888
                     /gene="pduC"
                     /locus_tag="J1Z28_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBA7716210.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIIHKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2898..3566
                     /locus_tag="J1Z28_001364"
     CDS             2898..3566
                     /locus_tag="J1Z28_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBA7716211.1"
                     /translation="MEINEKLLRQIIEDVLAEMQTSDKSVSFRAPVSATVSSVPDTEN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3581..4099
                     /gene="pduE"
                     /locus_tag="J1Z28_001365"
     CDS             3581..4099
                     /gene="pduE"
                     /locus_tag="J1Z28_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBA7716212.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPSASIQSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTAQDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLERRYQAQICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4109..5941
                     /locus_tag="J1Z28_001366"
     CDS             4109..5941
                     /locus_tag="J1Z28_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBA7716213.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALAETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGLTITPQELLTCPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGSNIGGMLEHVRQTMAELTNKPSHEIFIQDLLA
                     IDTSVPVNVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQKGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5931..6281
                     /locus_tag="J1Z28_001367"
     CDS             5931..6281
                     /locus_tag="J1Z28_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase"
                     /protein_id="HBA7716214.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDQEKLIVHYKNLPASAPLFTLMYQQDNHDRRSIGTNA
                     ARLVKGIPFRELHS"
     gene            6301..6576
                     /gene="pduJ"
                     /locus_tag="J1Z28_001368"
     CDS             6301..6576
                     /gene="pduJ"
                     /locus_tag="J1Z28_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBA7716215.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6600..7022
                     /locus_tag="J1Z28_001369"
     CDS             6600..7022
                     /locus_tag="J1Z28_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBA7716216.1"
                     /translation="MKQSLGLLEVSGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTNKALTVTVETSETP
                     ATTVISEIISCNLCLDPACQRQKGEPRSLCLHANKRGE"
     gene            7025..7657
                     /locus_tag="J1Z28_001370"
     CDS             7025..7657
                     /locus_tag="J1Z28_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBA7716217.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSTADYLRLFPE
                     QPISEKKLLMQPGQFAAEQVVTLEGPKGKLHHVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLVSPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7654..8145
                     /gene="pduM"
                     /locus_tag="J1Z28_001371"
     CDS             7654..8145
                     /gene="pduM"
                     /locus_tag="J1Z28_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBA7716218.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQEIVHQY
                     ASLQIRYVDLPLLRQLAENKTSDRAAIQIHEALAWGLHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHRDRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8149..8424
                     /gene="pduN"
                     /locus_tag="J1Z28_001372"
     CDS             8149..8424
                     /gene="pduN"
                     /locus_tag="J1Z28_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBA7716219.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8436..9443
                     /gene="pduO"
                     /locus_tag="J1Z28_001373"
     CDS             8436..9443
                     /gene="pduO"
                     /locus_tag="J1Z28_001373"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBA7716220.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYISTEEIAALENAIDSAMN
                     AVPPVHSFILPGRSEAASHMHFARTVARRAERRLVELTTETNVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPGA
                     PLYGLESYMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9440..10825
                     /gene="pduP"
                     /locus_tag="J1Z28_001374"
     CDS             9440..10825
                     /gene="pduP"
                     /locus_tag="J1Z28_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
                     /protein_id="HBA7716221.1"
                     /translation="MNNSELETLIRNILREQLVPATPRMQRNAIFQTVDEAVCTAHQA
                     FLRFQQCPLKTRSAIISAIREELMPRLTELAEESASETGMGNKEDKYLKNKAALDNTP
                     GIEDLTTTALTGDGGMVLFEYSPFGVIGSVTPSTNPTETIINNSISMLAAGNSVYFSP
                     HPGAKYVSLKLITTIEDIAFRCSGIHNLIVTVSEPTFAATEQMMAHPKVSVLAITGGP
                     GIVAMGMKSGKKVIGAGAGNPPCIVDETADIVKAAEDIINGASFDYNLPCIAEKSLIV
                     VASVAEYLIQQMQSFGALLLNHEQTEKLRAICLPDGSANKKLVGKSPSVLLEAAGLPL
                     PAKAPRLLIAVVDADDSWVTCEQLMPMLPIVKVNDFDSALALALKVEDGLHHTAIMHS
                     QNVSRLNLAARVMQTSIFVKNGPSWAGIGVGGEGFTTFTIATPTGEGTTSARTFARSR
                     RCVLTSGFSIR"
     gene            10836..11948
                     /locus_tag="J1Z28_001375"
     CDS             10836..11948
                     /locus_tag="J1Z28_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA7716222.1"
                     /translation="MKTFSLQTRLYSGTGSLNILSRFTNRHIWIICDVFLAHSPLIDT
                     LHQALPDSNRLSIFSEITPDPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGTGSEVTSACVISDSEKRIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     STRGKMHNASTLAGIAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRAAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLKTTCTLPTLTNTLKEKKAEWS
                     VRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11945..13288
                     /locus_tag="J1Z28_001376"
     CDS             11945..13288
                     /locus_tag="J1Z28_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex protein RnfC"
                     /protein_id="HBA7716223.1"
                     /translation="MNVGLTSCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARVDTFL
                     VNAAECEPMLKVDQQLMVQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTINGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLTVAKTVCEQCRLCTDLCPRHLVG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLIPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCIGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13291..13845
                     /gene="pduT"
                     /locus_tag="J1Z28_001377"
     CDS             13291..13845
                     /gene="pduT"
                     /locus_tag="J1Z28_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBA7716224.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSVASERAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13845..14195
                     /gene="pduU"
                     /locus_tag="J1Z28_001378"
     CDS             13845..14195
                     /gene="pduU"
                     /locus_tag="J1Z28_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBA7716225.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTVS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14200..14643
                     /gene="eutP"
                     /locus_tag="J1Z28_001379"
     CDS             14200..14643
                     /gene="eutP"
                     /locus_tag="J1Z28_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBA7716226.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYKKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     SMEQISLVKCWLREAGAHNVLVTSAVNNNGVTELFALLHTEEGCR"
     gene            complement(14744..15073)
                     /locus_tag="J1Z28_001380"
     CDS             complement(14744..15073)
                     /locus_tag="J1Z28_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBA7716227.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15244..16302)
                     /locus_tag="J1Z28_001381"
     CDS             complement(15244..16302)
                     /locus_tag="J1Z28_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBA7716228.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLDNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16500..16973)
                     /gene="sbmC"
                     /locus_tag="J1Z28_001382"
     CDS             complement(16500..16973)
                     /gene="sbmC"
                     /locus_tag="J1Z28_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBA7716229.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWIDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17092..18258)
                     /gene="dacD"
                     /locus_tag="J1Z28_001383"
     CDS             complement(17092..18258)
                     /gene="dacD"
                     /locus_tag="J1Z28_001383"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBA7716230.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQIITAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            18467..19894
                     /gene="sbcB"
                     /locus_tag="J1Z28_001384"
     CDS             18467..19894
                     /gene="sbcB"
                     /locus_tag="J1Z28_001384"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBA7716231.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(19968..21326)
                     /gene="plaP"
                     /locus_tag="J1Z28_001385"
     CDS             complement(19968..21326)
                     /gene="plaP"
                     /locus_tag="J1Z28_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBA7716232.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(21316..21378)
                     /gene="yoeI"
                     /locus_tag="J1Z28_001386"
     CDS             complement(21316..21378)
                     /gene="yoeI"
                     /locus_tag="J1Z28_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBA7716233.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(21593..22522)
                     /locus_tag="J1Z28_001387"
     CDS             complement(21593..22522)
                     /locus_tag="J1Z28_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716234.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(22568..23392)
                     /locus_tag="J1Z28_001388"
     CDS             complement(22568..23392)
                     /locus_tag="J1Z28_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7716235.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(23475..23729)
                     /gene="yoeB"
                     /locus_tag="J1Z28_001389"
     CDS             complement(23475..23729)
                     /gene="yoeB"
                     /locus_tag="J1Z28_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBA7716236.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(23726..23977)
                     /gene="yefM"
                     /locus_tag="J1Z28_001390"
     CDS             complement(23726..23977)
                     /gene="yefM"
                     /locus_tag="J1Z28_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBA7716237.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            24260..24310
                     /gene="hisL"
                     /locus_tag="J1Z28_001391"
     CDS             24260..24310
                     /gene="hisL"
                     /locus_tag="J1Z28_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBA7716238.1"
                     /translation="MTRVQFKHHHHHHHPD"
     gene            24456..25355
                     /gene="hisG"
                     /locus_tag="J1Z28_001392"
     CDS             24456..25355
                     /gene="hisG"
                     /locus_tag="J1Z28_001392"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBA7716239.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTDRLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            25361..26665
                     /gene="hisD"
                     /locus_tag="J1Z28_001393"
     CDS             25361..26665
                     /gene="hisD"
                     /locus_tag="J1Z28_001393"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBA7716240.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALCEYSAKFDKTTVTALKVSADEITAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAE
                     MARRVAEAVERQLAELPRAETARQALSTSRLIVTKDLAQCVTISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            26662..27732
                     /gene="hisC"
                     /locus_tag="J1Z28_001394"
     CDS             26662..27732
                     /gene="hisC"
                     /locus_tag="J1Z28_001394"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBA7716241.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQCVIDALRAEQV"
     gene            27732..28799
                     /gene="hisB"
                     /locus_tag="J1Z28_001395"
     CDS             27732..28799
                     /gene="hisB"
                     /locus_tag="J1Z28_001395"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBA7716242.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFEGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            28799..29389
                     /gene="hisH"
                     /locus_tag="J1Z28_001396"
     CDS             28799..29389
                     /gene="hisH"
                     /locus_tag="J1Z28_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBA7716243.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPRMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            29389..30126
                     /gene="hisA"
                     /locus_tag="J1Z28_001397"
     CDS             29389..30126
                     /gene="hisA"
                     /locus_tag="J1Z28_001397"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBA7716244.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVATLLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQKNSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            30108..30884
                     /gene="hisF"
                     /locus_tag="J1Z28_001398"
     CDS             30108..30884
                     /gene="hisF"
                     /locus_tag="J1Z28_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015853929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBA7716245.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            30878..31486
                     /locus_tag="J1Z28_001399"
     CDS             30878..31486
                     /locus_tag="J1Z28_001399"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBA7716246.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(31525..32640)
                     /locus_tag="J1Z28_001400"
     CDS             complement(31525..32640)
                     /locus_tag="J1Z28_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004184789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA7716247.1"
                     /translation="MRVLHVYKTYYPDTYGGIEQVIYQLSQGCARRGIAADVFTFSPD
                     KDTGPVAYEDHRVIYNKQLFEIASTPFSLKALKRFKLIKDDYDIINYHFPFPFMDMLH
                     LSARPDARTVVTYHSDIVKQKRLMKLYQPLQERFLSGVDCIVASSPNYVASSRTLKKY
                     LDKTVVIPFGLEQQDVQHDPQRVAHWRETVGDKFFLFVGTFRYYKGLHILMDAAERSR
                     LPVVVVGGGPLESEVRREAQQRGLSNVMFTGMLNDEDKYILFQLCRGVVFPSHLRSEA
                     FGITLLEGARFARPLISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(32650..>33783)
                     /locus_tag="J1Z28_001401"
     CDS             complement(32650..>33783)
                     /locus_tag="J1Z28_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBA7716248.1"
                     /translation="FATEPIKYPLTGIGRYSLELVKRLAVAREIEELKLFHGASFIEQ
                     IPLVENKSDTKASNHGRLSAFLRRQTLLIEAYRLLHPRRQAWALRDYKDYIYHGPNFY
                     LPHKLERAVTTFHDISIFTCPEYHPKDRVRYMEKSLHESLDSAKLILTVSDFSRSEII
                     RLFNYPAERIVTTKLACSSDYIPRSPAECLPVLQKYQLAWQAYALYIGTMEPRKNIRG
                     LLHAYQLLPMEIRMRYPLILSGYRGWEDDVLWQLVERGTREGWIRYLGYVPDEDLPYL
                     YAAARVFVYPSFYEGFGLPILEAMSCGVPVVCSNVTSLPEVVGDAGLVADPNDIDAIS
                     AQILQSLQDDSWRDIATARGLAQAKQFSWENCATQTINAYKLL"
BASE COUNT         8536 a         8803 c         8612 g         7832 t
ORIGIN      
        1 ttattatatt tgttctctat taatttttct cattagccga ttatgtcagc cattcttttt
       61 cctcgctttt ttttatagtc atatcgtcaa aaaaatccag gcgaggtctt tatgcaacaa
      121 gaagcgttag gaatggtaga aaccaaaggc ttaactgccg ccatagaggc cgcggacgcc
      181 atggtgaagt cggcaaatgt tttgctggtc ggctacgaaa aaataggttc cgggctggtg
      241 accgtcatcg ttcgtggtga tgtcggggcg gtaaaagcgg caacagacgc gggtgccgca
      301 gctgcgcgta acgtcggcga agtaaaagcc gtacacgtta ttccccgccc acataccgat
      361 gtcgaaaaaa tcttaccaaa gggaattaac ccatgagcag caatgaactg gtcgaacaga
      421 tcatggcgca ggtcatcgct cgcgtagcga cgccagagca tgaggttctc ccgaaaacca
      481 ctcatcaaat acgagagacg gctatggcag aaaaaagctg cacattaaca gaatttgttg
      541 gcaccgctat tggcgacact gtaggtctgg tcattgcgaa cgttgatagc gccctgttgg
      601 acgcgatgaa acttgaaaaa cgctatcgtt ccattggcat tctgggggcg cgtattggcg
      661 cagggccaca cattatggcc gctgatgaag cggtaaaagc caccaacact gaagttatta
      721 gcattgaact tcctcgcgat actaaaggcg gtgccggaca cggctcattg atcctcttag
      781 gtggcaacga tgtttccgac gtgaaacgcg gtattgaagt tgccctgaaa gaactcgatc
      841 ggacctttgg tgatgtctat gccaacgaag ccgggcatct tgaactgcaa tacaccgccc
      901 gcgccagcta tgcactggaa aaagcgtttg gcgctccact gggacgggct tgcggcgtta
      961 ttgtcggcgc tccggctgcg gtgggtgttc taatggccga caccgcgctg aagtccgcca
     1021 atgtggatgt cgtggcatac agctcccccg ctcaaggcac cagctacagc aacgaagtca
     1081 ttctgattat ctcgggtgac tccggcgcag tccgccaggc ggtcatctcc gcacgcgaaa
     1141 tcggtaaaac cgtgctttcg actctcggcg atacaccgaa aaacgatcgc ccttcctaca
     1201 tctgaattct ctgaggcaga tttatgagat cgaaaagatt tgaagcactg gcaaaacgcc
     1261 cggtcaacca ggacggtttc gttaaagagt ggattgaaga aggcttcatt gcgatggaaa
     1321 gcccgaacga tcctaaaccg tcaatcaaaa ttgttaacgg cgtcgttacc gaactggatg
     1381 gaaaacccgc cagccagttt gacctgatcg accactttat cgcccgctac ggcatcaatc
     1441 tcgcgcgcgc cgaagaagtc atcgcgatgg attcggtaaa acttgccaat atgttgtgcg
     1501 atccaaatgt caaacgtagc gatatcgttc cccttaccac agcaatgacg cctgcaaaaa
     1561 tcgtcgaagt ggtttcgcag atgaacgtgg tagagatgat gatggcgatg cagaaaatgc
     1621 gcgcccgtcg aacaccatca caacaggctc acgtcaccaa cgttaaagat aacccggtac
     1681 aaattgccgc cgacgcagct gaaggcgcat ggcgcgggtt tgatgaacag gaaaccaccg
     1741 ttgccgttgc tcgctatgca ccgttcaacg ccatcgccct gctggtcggc tcacaggtgg
     1801 gacgtccggg cgtcttgacc cagtgttcac tggaagaagc caccgaactg aaactcggta
     1861 tgttaggcca cacctgttac gccgaaacaa tttctgtcta tggtacagaa cctgttttca
     1921 ccgatggcga tgataccccg tggtcaaaag ggtttctcgc gtcatcttat gcttctcgcg
     1981 gcttaaaaat gcgctttact tccggctccg gctcagaagt acagatgggt tacgcggaag
     2041 gcaaatcaat gctttatctc gaggctcgtt gcatttacat cactaaagcc gcaggcgttc
     2101 agggtctgca gaatggttcg gtaagctgca tcggtgttcc atcagctgtg ccatcaggca
     2161 tccgcgcagt attagcggaa aacttaattt gctcatcgct ggatctggaa tgcgcctcca
     2221 gtaatgacca gacctttacc cactcggata tgcgccgtac cgcacgtttc ctgatgcaat
     2281 ttctgccagg taccgacttt atctcttccg gtttttccgc tgtgccgaac tacgacaaca
     2341 tgtttgctgg ttcaaacgaa gatgctgaag acttcgatga ttacaacgtc attcagcgtg
     2401 acctgaaggt tgatggcggg ctgcgaccag tacgcgaaga agatgttatc gcaatccgca
     2461 ataaagccgc tcgcgcttta caggctgtct ttgccggtat gggactaccg ccaattaccg
     2521 atgaagaagt cgaagcggca acctatgccc acggttcgaa agatatgcct gagcgcaata
     2581 ttgtggaaga catcaagttc gcccaggaga ttatccataa aaaccgtaac agcctggaag
     2641 tggtgaaagc cctggcgcaa ggcggtttta ccgatgtcgc ccaggacatg ctcaacatgc
     2701 aaaaagcaaa actgaccggc gattatctcc atacctcagc catcatcgtc gatgacggac
     2761 aagtgctctc tgcggtcaat gacgtcaatg attacgccgg accggctaca ggttaccgcc
     2821 tgcaagggga acgctgggaa gagatcaaaa atattcccgg tgcacttgat cccaacgaaa
     2881 ttgactaagg gtgagaaatg gaaattaacg aaaaattact gcgtcagatt atcgaagacg
     2941 tgctggcaga aatgcaaacc agcgacaagt ccgtttcatt tcgcgcacct gtctctgcaa
     3001 ctgtctcttc agtgccagat accgaaaatt ttctgaccga aattggcgaa gcccaacaag
     3061 gcactcagca agatgaagtc attattgctg taggcccggc gttcggcctg gcgcaaaccg
     3121 tcaatatcat cggtattccg cataaaaaca ttttgcgcga agtgattgcc ggtatcgaag
     3181 aagaaggcat taaagcccgt gtgattcgct gctttaaatc gtccgatgtc gcattcgtag
     3241 cagtcgaagg agatcgtctt agtggttcag gtattgctat cggtattcag tcgaaaggca
     3301 caacggttat ccaccagcag ggacttcctc ccttgtctaa cctggaactt ttcccacaag
     3361 cgcctttgtt gacgctggat acttatcgtc agattggcaa aaacgccgcg cgttatgcga
     3421 agcgcgagtc tccacaacca gttccgaccc tcaacgatca aatggcgcgg cctaaatacc
     3481 aggccaaatc agccattttg catatcaaag agacgaagta cgtggtcact ggcaaaaaac
     3541 cgcaagaact gcgcgtgacg ttttaacaaa ggaaaccttc atgaataccg atgcaattga
     3601 gtcgatggtc agggacgtgt tgaaccgcat gaacagttta caggatgcag ctcctgtttc
     3661 tgcggtcccc agcgcgtcaa tacagagcgc caaagtgacg gattacccgc tggcaaataa
     3721 acatccggaa tgggttaaaa cagcaaccaa taaaacgctc gacgatttca ctctggaaaa
     3781 tgtgctcagc gataacgtca ccgcccagga tatgcgcatt acccctgaaa cattgcgtat
     3841 tcaggccgcg attgccagag atgctggtcg tgatcgcctg gcgatgaatt ttgaacgcgc
     3901 cgctgagctt acctcagtac cggatgatcg catcctcgaa atctacaatg cgttacgccc
     3961 ttatcgttcg actaagcaag aacttattgc cattgctgac gatcttgaac ggcgttatca
     4021 ggcccaaatt tgtgctgcat ttgtccgtga agctgcggaa ctttatgtcg agcgcaaaaa
     4081 actgaaaggc gacgattaag gacgaattat gcgatatata gcaggcattg atatcggcaa
     4141 ctcatcaaca gaggttgcgc tggcaacgct cagtgcaact ggagagttgt cgttcgtgag
     4201 tagcgccctg gcggaaacca ccggaattaa aggtacgttg cgtaacgtcc atggtattca
     4261 ggaggcgctc gcacaggcaa cgaaaaaagt cggcatcaat gttagcgata tttcgcttat
     4321 tcgtatcaac gaagcaactc cggtcattgg tgatgtggcg atggaaacca tcactgaaac
     4381 tatcatcacc gaatcaacca tgatcggtca caacccgaaa acgccgggcg gagtggggct
     4441 gggcgtaggg ctcaccatca ctccgcaaga gttattaaca tgcccggcgg acacacccta
     4501 cattctggtg gtgtcatcgg catttgattt tgccgatatc gcaacaatga taaacgcctc
     4561 cgtacgagcc ggatatcagc tgactggcgt tattctgcaa cgcgacgatg gcgtactggt
     4621 caataaccgc ctggaaattc ctctccctat tgttgatgaa gtcctctaca tcgatcgtat
     4681 tccgctcggc atgctggctg cgattgaagt tgccgttccc gggaaagtta tcgaaaccct
     4741 gtctaacccg tatgggattg ccaccgtttt cgccctcaat gctgaagaga ctaaaaatat
     4801 tgttcctgtc gcgcgggcgc taatcggcaa ccgttcagca gtggtagtga aaacgccttc
     4861 tggcgacgtt aaagcccgtt cgatacccgc cggaaatatc gaactgcttt ctgccgggcg
     4921 cacaacgcgg gttgatgtag ctgctggtgc cgatgccatt atgaaagcgg tcggtgagtg
     4981 cccaaaactg gaaaacgtga cgggagaacc tggctccaac atcggcggaa tgctggaaca
     5041 tgttcgccag accatggctg aactgaccaa caaacccagc cacgaaattt ttattcagga
     5101 tttgctggcg atagatacct ctgtccccgt caacgtaacc ggtggtctgg caggtgaatt
     5161 ttcactggaa caggctgtgg gtattgcctc tatggtgaaa tccgaccgtc tgcaaatggc
     5221 gatgatcgcc agtgaaatta agcaaaaact gcatgttgat gttcaggtcg gaggcgcaga
     5281 agcagaagcc gccattcaag gggccttgac tacccctgga acgacacgtc cactagcaat
     5341 tctggactta ggtgcaggct ctaccgatgc ctccatcatc aatcagaaag gggaaatagt
     5401 tgccactcac cttgcgggag cgggcgacat ggtaacgatg attatcgctc gtgagctggg
     5461 cttaaatgat cgttatctcg cagaagaaat caaaaagtac ccgttggcaa aagtagaaag
     5521 cctgttccac ttacgccatg aagatggcag cgttcagttc ttccctactc cgctgtcccc
     5581 tcatgtattt gcccgcgttt gtgtggtgaa acctgacgaa ctggtgccta ttcccggtga
     5641 tttgacgctg gaaaaagtac gcgctgtccg tcgcagtgcg aaagagcgtg tcttcgtaac
     5701 taatgccctg cgtgcgctgc gtcaggtaag ccctgccgga aatattcgcg acattccgtt
     5761 cgtcgttctg gtgggtggct cttcgctgga tttcgaagta ccgcagttag tcaccgatgc
     5821 attggcgcat tatcgattgg tcgccggacg ggggaatatc cgtggtagcg aaggtccgcg
     5881 caacgcggta gctactgggc tactcatcgc ctggcacaag gagtcgattc atggaaagta
     5941 aagagcgtgc accagccatc gttgtcatgg agatcggtga ctgtatcact acatgggatg
     6001 aagtgcttct tggcattgaa gaggaaggta ttccctttcg cattcaacac attccgtcag
     6061 gtgaagttat cgatagcgcc tggctggcgg ctcgtcaatc tcccctgttg gttggcattg
     6121 cctgcgatca ggaaaaactg attgtgcatt acaaaaactt accggcatca gcgccactgt
     6181 ttacgctgat gtatcaacaa gataaccatg acaggcgcag catcggcact aacgctgccc
     6241 gcctggtaaa aggaattccc ttccgggaac tccactcata atccacagga gaaacgcagt
     6301 atgaacaacg cactaggact ggttgaaaca aaagggttag ttggcgcaat tgaagctgca
     6361 gatgccatgg ttaaatccgc caacgtgcag ctggttggct atgaaaagat tggttcgggt
     6421 ctgattacgg tgatggtccg tggcgatgtt ggcgcagtaa aagccgccgt tgatgcagga
     6481 agcgccgccg caagcgccgt tggcgaagtg aaatcctgcc atgtcatccc gcgcccacac
     6541 agtgatgttg aggctatttt acctaaatca gcataacccc attcgctaag gagcacagcg
     6601 tgaagcaatc actgggatta cttgaagtca gtggtctggc actcgccatc agttgcgcag
     6661 atgctatggc aaaagccgcg tccatcacgc tggttggcct tgagaagacc aatggctcag
     6721 gctggacgct catcaaaatc accggtgacg tcgcctcggt taatgcggcg atcgccacgg
     6781 gagccagttt cgccggacag cgaaatggcc tcgtcgcaca caaagtgatt gcccgcccgg
     6841 gagacggcat tctgccccac acaaataaag cattgactgt gacggttgaa acaagcgaaa
     6901 caccagcaac aaccgtcatc tcagagatta tcagttgtaa tttgtgtctt gacccggcat
     6961 gtcagcggca aaaaggtgag ccccgctcac tttgcctgca cgccaataag cgaggggagt
     7021 aacagtggat aaacaaacct tgcaggaaac agtgagccag attctggatg agatgcgcga
     7081 gcgccctatc cctctgggtg tttccagtcg tcatattcat ctttcaacag cggattattt
     7141 gcgtcttttt cctgaacaac cgattagcga gaaaaagctg ttaatgcaac ctggccaatt
     7201 cgctgctgaa caggttgtta cgctggaggg accaaaaggt aaattgcacc acgttcgctt
     7261 gttggggccg ctacgcaatg caagccaggt ggaaatttcc cgtacggacg ccagaacgtt
     7321 agggatcacc gccccgctac gcatgtcagg cgatctcagc ggcactccag ggattcgttt
     7381 ggtaagccca tttgcggaac tggagatcac atctggcgtc attgtcgcgc aacggcatat
     7441 tcatatgtct ccgctggatg ccctgattta tggcgttacg catggcgata aagtggcggt
     7501 tgccattgaa ggtgatgagc gacgactgat attcgaaaac gtcgctgttc gcgtctctcc
     7561 ggatatgcgt cttgaaatgc atattgatac cgatgaagcc aacgccgcag gggctgatag
     7621 ccctgacgtt tttgccacac tggtgtcagt gccatgaata acatgctgat gcaacgtatt
     7681 gttgacgaag tggtttttcg ccttaaacaa cgtgcgggaa aaacactcgt gctgactgtt
     7741 tttcaactgc gagacgccag cgttcaagag atagttcatc agtatgcatc attacaaatc
     7801 cgatatgtcg atctgccgtt gttacgacaa cttgcggaga acaaaacgtc agatcgagca
     7861 gcaattcaga tacacgaagc gttagcctgg ggattgcaca ttcaattgtc gttgcagcgt
     7921 catttcctga acgccattga attgaagact ctcgcccgtc taccgctaag ctggtgcgac
     7981 gagcaagggc aaccgatcta ccttcatcgt gatcgacttt taagttatgc cgatattgca
     8041 cagttaagct cgggaatact ggtgttacag cgaaaatgtt gtgtgaccgc actggctcgt
     8101 gaagcagcaa ttacccgaaa tattcagtta atcaggcagg agtaacctat gcatctggct
     8161 cgagtcactg gcgcggtagt atccacgcaa aaatcacctt ctctgatcgg gaaaaaactg
     8221 ctgctggttc gccgggtcag tgctgacggc gaactgcctc tcacgccatt aaccggcgac
     8281 gaagttgccg ttgattccgt cggcgcaggc gttggtgagt tggtactcct cagcagtggt
     8341 tccagcgcca gacatgtgtt ttccggccct aatgaagcca tcgacctggc agtggtcggc
     8401 attgtcgata cgctttcgcg ctaaggagct gaaagatggc aatttatacc cgaacaggtg
     8461 attcaggaag tacgtcttta tttaccggac agcgcgtcag caaaactcat ctacgtgtcg
     8521 aaacctatgg cacgctggat gaactgaacg cgacactcag tctgtgttat tgcgccaccg
     8581 ccatcgaaag ccatcgcatc ttactggaag ccattcaaca acagatattt tggtttagcg
     8641 ccgagctcgc gagtgaaagc gaacagccgt cagcgcagca gcgttacatc agtacggaag
     8701 aaattgctgc gctggaaaac gcgatcgata gcgcaatgaa cgccgtcccc ccagttcaca
     8761 gcttcattct gcctggtcga tctgaagccg caagccacat gcatttcgcc cgcacagtgg
     8821 ctcgacgggc tgaaagacgt ctggttgaac tgacaacgga aaccaacgtc cggaatgttt
     8881 tgctgcacta tatcaaccga ctttccgatt gcctgtatgc cctcgcccga gtggaagaca
     8941 acgttgctca tcaaaacctg atgattcagg aaatcacaaa acgttatcac gaagccaacc
     9001 acacaccagc attgaaggaa cgcacaatgc cgctcacatt tcaggatctt caccagctca
     9061 ttcgtagtgc ggcaatgcgc gcggatgaac tgcatattcc cgttgtcatc agcattgttg
     9121 atgccaacgg aacagaaagt gttacctggc ggatgcctga tgccctgttg gtcagcagtg
     9181 aactggcacc gaaaaaagcc tggactgccg tggcgatgaa aacggcaacc cacaaacttg
     9241 ctgataccgt gcaacccggc gcgccgcttt acgggctcga aagctatatg caaggcaaag
     9301 tcgtcacttt tggtggcggc ttccccctct ggcgtgacgg aaaattgctt ggcgggcttg
     9361 gtatcagtgg cggtagcgtt gaacaagaca tggatattgc ccaaagcgca atggcggcaa
     9421 ttaacgtggg agtaaaccaa tgaataactc tgaattagaa acactgatcc gcaatattct
     9481 gcgtgaacaa cttgtccccg ctacaccacg aatgcaaaga aacgctattt tccagactgt
     9541 tgatgaagcg gtctgcacag cacaccaggc ctttttacgc tttcaacagt gcccgcttaa
     9601 aacccgtagc gccattatca gcgccattcg cgaagaatta atgccccgcc ttaccgaact
     9661 ggcagaagaa agcgccagtg aaacagggat gggaaataaa gaggataagt acctaaaaaa
     9721 caaagcggca ctcgataata cgccaggaat tgaagattta accaccactg cactaaccgg
     9781 tgatggtggc atggttttgt ttgaatactc cccgtttggg gttattggct cggtcacgcc
     9841 aagcaccaac cccacggaaa ccatcattaa caacagcatc agcatgctgg cagcaggtaa
     9901 tagcgtctac ttcagtccac acccgggagc caaatacgtt tcgttaaaac tgattaccac
     9961 aatcgaagac atcgcgtttc gctgtagtgg cattcataac ctgatcgtta ccgtatctga
    10021 accgaccttt gctgccaccg aacagatgat ggcgcaccct aaagtcagcg ttctggcgat
    10081 taccggcggc ccggggatcg tcgccatggg aatgaaaagt ggcaagaaag tgattggtgc
    10141 cggagcgggt aaccctccgt gtatcgtcga tgaaacagcg gatattgtga aagccgccga
    10201 ggacatcatc aacggcgctt catttgatta caacctgccg tgtatcgctg aaaaaagcct
    10261 gattgtcgtg gcgtcagtgg cagagtattt aattcaacag atgcaaagtt tcggcgcact
    10321 tcttcttaac catgaacaaa cggagaaatt gcgtgcaatt tgcctgccag acggtagcgc
    10381 caacaaaaaa ctggttggga aaagcccatc agtcttgctg gaagcagcgg gtctcccttt
    10441 gcccgcaaaa gcgccgcgcc tactgattgc tgttgttgac gccgatgata gttgggttac
    10501 gtgtgaacaa ctgatgccaa tgctgcccat tgtgaaggtc aacgattttg acagcgctct
    10561 cgccctggcg ttgaaagtgg aagacggctt gcaccacacg gcaattatgc actcgcagaa
    10621 cgtctcacgg ctcaaccttg ccgcgcgcgt tatgcaaacc tcaatctttg tcaaaaacgg
    10681 cccctcctgg gcgggtattg gcgtcggcgg cgaaggtttt acgaccttta ccattgcaac
    10741 accgaccgga gagggtacaa cgtcagcacg gacttttgcc cgctcacgac gctgcgtgtt
    10801 aaccagcggc ttttccattc gctagagaaa cggaaatgaa aactttttca ctgcagacgc
    10861 gtttatacag cggaacggga agtctcaaca tactgagccg tttcacgaat aggcacatct
    10921 ggattatttg cgacgtcttt ctggcacact caccactcat tgacacgttg catcaggcgc
    10981 tgcccgacag taatcgtctc agtattttta gcgaaataac gcccgaccca actatccaga
    11041 ccgtggtgaa aggcattgcg caaatgcaga ctttacgccc ggatgtggtt atcggttttg
    11101 gcggcggttc tgccctggac gccgcaaaag cgattgtctg gttcggtcgc cagtgtggca
    11161 ttgaaattga aacctgcgta gccattccta ccaccagcgg taccggttcc gaagtgacca
    11221 gtgcttgtgt catcagcgat tctgagaaaa ggattaaata tccccttttc gataatgcac
    11281 tttatccgga tatcgcgatc cttgatccat ctttggtcgt cagcgtgcca ccagcgataa
    11341 cggccaatac cgggatggat gttttaaccc atgcgcttga agcttatgtt tctccccgcg
    11401 ccagcgactt cacggatgca ctggtcgaaa aagccgtgca aattgttttt caatacctgc
    11461 ccactgcagt gaagaaaggc gattgtcttt ccacccgtgg caaaatgcat aatgcatcga
    11521 cgcttgcagg aatagccttt agccaggccg gactcggttt aaaccatgca attgctcatc
    11581 aactcggcgg acagtttcat ctgccgcacg gcctggcgaa tgcgctcctg ctcacggcgg
    11641 taatccgctt taatgctggc gatccgcgcg cagccaaacg ctacgcccgg ctggcgaaaa
    11701 cgtgccattt atgcccggac aacgccaatg acaccgccag cctgaatgca cttattcaac
    11761 atattgaaca gcttaaaaca acctgtacgc tgccgactct taccaatacg ctgaaagaaa
    11821 aaaaagcaga atggtcagtt cgcataccag atatggttca ggcggcactg gcggatgcaa
    11881 cgttgcgtac caacccgcgt gcggctgatg cctccgcgat tgcagaactg ctcgaggagt
    11941 tgttatgaac gtcggattaa cctcctgcga cgaccagacc atccgcgagc gagtacaagc
    12001 agcaggtgtc gtgggtgctg gcggggcagg ttttccaacc catattaagc tacaagcgcg
    12061 tgttgacacg ttcctggtga atgcggcgga atgcgaacca atgttgaaag tggatcaaca
    12121 gttaatggtg cagcaagcca gccgcctgct tcgcggtgtt cactatgcga tgaaagccac
    12181 cggggccagt agcggaatca tcgcgctgaa agagaaatat caacgggcaa taaacgcact
    12241 cacaccactg ttaccaccgg atattcgcct gcatattttg cccgatgttt accccgcagg
    12301 agacgaagtg ctgaccatct ggatggcaac tggtcgccgc gttccacccg ctgcattacc
    12361 cgttagcgtc ggtgtggtgg tcaataatgt tcaaaccgta cttaatattg ccagagccgt
    12421 cgagcaacag tatccagtga cgcgccgcac actcactatt aatggtgcag tggcgaaacc
    12481 aataacggta accgtcccca tcggcatgtc tttacgtgaa gtcctggcgc tggcgggcgg
    12541 cgcaacagtc gatgcgcctg gttttatcaa tggcgggccg atgatgggaa gtttgatcac
    12601 atcgctcgac acacccgtca gcaaaaccac tggcggcttg ctggttttac ccaactctca
    12661 ttcactgatc caacgtcgaa tgcagaatga ccgcagcgtg cttaccgtcg cgaaaactgt
    12721 ttgtgagcaa tgccgcttgt gtaccgatct ctgcccacgt catttagtcg gtcacgaact
    12781 ggcaccccat ttattagtac gtgcggttaa ctaccaacag ctcgctacgc cacaactgtt
    12841 actgacggcg ctcacctgtt cggagtgcaa cgtctgcgcc agcgtcgcct gtccggtagg
    12901 aatttcacca atgcgcataa accgcctgct taagcaggaa ttacgcgcac aaaatctccg
    12961 ctacgaaggt gccttaaatc ctgcggatcc catggcaaat tatcgactga ttcccgtaaa
    13021 acggctggta accagactgg gcctaatccc gtggtatcag gatgcgccat tgtctgaaca
    13081 ggtgcctcag cccgaaaaag tgacgttatt gttacgccag cacatcggtg ccagcgcaat
    13141 tgcgtgtgta caaaaaggcg atcgcgtcgt tcatgggcaa tgtataggtc aaattccaca
    13201 cggcacatta ggtgccccca ttcatgccag tattgatggc atggtcagcg atgtgacaga
    13261 aaacgcgatt acgcttgtga gaggttaacg atgtcgaaag cggtaggtat tttagaactt
    13321 agcagtatcg cgaaagggat ggaaaccggc gacgccatgc tgaaaagcgc aaacgttgag
    13381 ttactggtca gcaaaacgct atgccccgga aaatttctgc tgatgctcgg tggcgatgtg
    13441 ggtgcggtgc aacaggccat caacagcggt gcttcccttg ctggtgaaat gctggtcgac
    13501 agtcttgtcc tgccgaatat tcatcccagt ctcctttctg caatcagcgg actgaaccat
    13561 gttgagcagc accgggctgt cggcgtagtc gaaacctgga gtgtcgctgc ctgcattgac
    13621 gctgccgatt gtgctgtcaa agctgcgaat gtcattctcg ttcgcgtgca tatggctttt
    13681 ggcattggcg gaaaatgcta catggtggtt gctggcgata tttcagatgt taataacgcg
    13741 gtctctgtcg ccagcgagcg tgcgggagaa aaaggtttgt tggtctatcg gtcggtgatt
    13801 gcccgtccac atgaagccat gtggcgtcag atggtggagg gataatgaca actcagcaca
    13861 caactgaacg gatgattcag gaatatgtcc ctggtaagca ggtcacgctg gcacatgtca
    13921 tcgccaatcc tgggaaggat ctgtttaaga aattgggttt accagacacc gtttctgcta
    13981 ttggcattct gacgattacc cccagcgaag catctatcat cgcatgcgat atcgccacaa
    14041 aatcaggcgc tgtagagatc ggttttcttg atcgttttac cggtgccgta gtgctgactg
    14101 gtgatgtttc tgctattgaa tatgcactta gccaggttac ccgtacgctg ggtgaaatga
    14161 tgcgctttac cgcctgcccc attacgcgga cctgacgtta tgcaacgtat gatgcttatc
    14221 ggccccagcc agtgcggaaa aacgtcactc acgcaatgtc tgcgtggcga aaatatccat
    14281 tataagaaaa cgcaggcaat cgtctggtcc ccgaccacca ttgatacacc aggcgaatac
    14341 ctcgaaaacc gcagcctcta cagtgcattg ttggtcagtg cctgcgaagc agacattatc
    14401 gcgctggtac ttaatgcgga cgcaccatgg tcaccttttt caccaggctt tacctcgtca
    14461 atgaatcgcc cggtcattgg agtagtaacc aaagcagatt tagccagtat ggaacaaata
    14521 tctttggtga aatgttggtt acgggaagct ggagcgcaca acgtgttagt aaccagtgcg
    14581 gttaataaca atggagttac agaacttttt gccttgctgc atacagaaga aggctgtcgc
    14641 taatttcgat aggttctcag atgagaatga tgacggtgat acacagataa gaatcaatga
    14701 gcaatgaggg ttgccgggca accctcattg aataaaacag gaattacttc gcttcgccgt
    14761 ttttcatttc gcccatagct ttcagctttt tggagatatc gcggcgttct ttggagagct
    14821 cggcattttt gatgatgtaa tcgtcaacgc gatcttcata gtcacctttc atgctggcga
    14881 tgatgccctg gattgcttca acgctcatcc ctggcttgat gtaatcgctc aggttgtcca
    14941 gcaacaggac gcgcttctgg ttgtcacgga tctttttctc aacgtcctga atttcacgtt
    15001 gcagtttgtt cttacgacgg aacagacgaa caaattccag tacgtcctgg aatgaaggct
    15061 tggtagtttc catttttata cccctgataa tgtgagagtc ggattcgttt aagcaaccgc
    15121 tattcgttag ggccaacatt actcatagct gccgcagatg acaatgcttt tatccctttt
    15181 cactatcata ccctttatcc ttgctgaatc gaagcagcag caagatgatt ctgaagtcag
    15241 gaattatttg cgcaaggccc ggcaaatcag attcgatagc aattcaagct gatgcgctgt
    15301 ttccatgttc agccacacat aaccatagat tggtgtttcc acgactttca ggtcatggtg
    15361 gttatcgagc aactgacgca gctcttccac agcatcgttc aatttttccg tattggcaaa
    15421 aaccggctgc ggattacctt cgtacagcgc atgaacaagg ctcagcagta tttgctgcat
    15481 catgtgctgg gtatcacgaa gtttttgcgc gttcaataac acgaaatggc tggggcgcgt
    15541 ggcccagtat gcattgattt gcaactccag catacaaacc agattgcgat taatggtctg
    15601 gataccttca tatatcgatt tgggaatacg ggtttctttg ctggcgggcg caatcagccc
    15661 acgcattttc acggcatcgg tcaggagttt ttgtagatgg ctttccagac gtgggcgttc
    15721 gagtaagttc ggtgagaatg cagattgata gacccgatta tactcggtca gacttttcgc
    15781 cagttgaatg cgccagtgga tgaatgcccg ttgtggccag ataccggtaa acaacattgc
    15841 cagtaaagag ccgaggatca catcgccgct tcgccataac gccgtatcaa tttcacctgt
    15901 cggggaaccc acaacaattg ccagcgtcac cccaatcaat aatccttgat acggtttctt
    15961 gcccagcgcc agccaaccgc aaagaaacat cgccgccgcg caccagacta acatcagcgg
    16021 taacgaaatt aactccagtt gcagagcgat aagacctaaa atcgacccca acaccgtacc
    16081 gccaatacgt tcgaaggcac gagggacaac gttcccccag aacgagattg gccccataat
    16141 caccaccatg gtgaccagcg gccaggtgct ttccgggata gtaaacaggc ggataatgag
    16201 aaaagtgagc aggaatgcca gcgcgacccg agtaccatgc acaatgcggt aatggcggta
    16261 tacccggatt tcaaacgggc ttaatgactt atcggcacgc acgcagaaac tccgtaatga
    16321 agattaaaag cgctcataac acatctgtcg gccagaaggc gcgcctctga gttatccgac
    16381 ggacataccg ggaacagtgt aatttcccgg caggagcgaa aaatttgaaa ggcgcatctt
    16441 actcttttcg ctttcaaaaa aagagtggtc ttcgcgttaa cacaccgccc tgagatgaat
    16501 tagtgatgtt ttggctgcac cgcaacatac atttcgatat cccagtaccc atcttccgcg
    16561 ccattgttca agtaaacctc gaagcagggc tttggtaaca tttcataagc actgtcctgc
    16621 aagaggctat taaagaactg ataccagggt ttagcaaaat catcaccgac tacacgagcc
    16681 accgccaccg catactgacc acctgaaatt tctgtcagaa tgacgccctc actgttttcg
    16741 gggagcgtaa agttattcgg taccgtcacg acggtgtcgc agcgtaattt ttcggcgggt
    16801 gtttcatctg gattgtcgta atagacagca acccactcct tcggcacaat atttttgcta
    16861 tctatccaca tcatcaactg ctcaaaacct ttctttaccg tctgctccca cgggccaaca
    16921 agatggaaac ctgcaacggt acgtttatct tcctgcttaa tctcgtagtt catgacgcct
    16981 ccactaacac tgtttttata tacagtatag ttgcaatatt aaaaccacaa ggaatgagtg
    17041 ttgattatgc gagcagactc gcactcctgc cagtctgctg caaaagaaag gtcaggcctt
    17101 atggtggaaa taatcactca ggcgggaaaa catgctgcct tccccgacag attccagggt
    17161 aaccagcggc cagtgcgcca cctgtttatc acggtcgtaa agttcaattt cccctacccg
    17221 ctgatgggcg ctaattggcg cggtgagttc tttaccatca agggtatatt tggctttgat
    17281 atgtggaatt tcggctttcg gtagcaccat ccagaactct tgttccgttc ccagggcgat
    17341 attttcttta tcaccatacc agatgcgttc cgtaccgacc tttttcccac ggtgcaaaat
    17401 ttgcaccgta gtaaagtttt gttgccccca acgcagtaat tttcttgcct cttcctcacg
    17461 accttttgca ctgtcagccc ccattaccac tgcaatgaga cgacgctgcc cgtccacagc
    17521 cgaagcaata agattaaacc cggcaccaga agtatgaccc gttttcaggc cgtcaacatt
    17581 catggtctta tcccacaata acccgttacg gttttgctgg gtgataccgt tccaggtgag
    17641 gcttttctca ctgtacatat gataaaactc gggctcgccg tggatgatag cgcgagaaag
    17701 cacagctaaa tcataagccg agctatgctg gccaggtgca tccagaccat gcactgtttc
    17761 aaaatgcgta tccttgagat gcagcttctc ggcatagttg ttcatcattt caacaaactg
    17821 ccgttgccca ccggcaatat agtcagccag agcaacacaa gcgtcatttc cggaatccac
    17881 aattaaacca cggcttaaat cacgtaccga tacgcgatcg ccctctttca aaaacatcag
    17941 tgaagaaccg acaaacaccg gattatcttt cgcccacgca tcacgcccca cggtgacaat
    18001 atcgtctggc gtaatgcgat gactatcgat agcgcgatcc acgacatagc ccgtcatcag
    18061 ctttgtcagg ctggcgggat tgcgctgttg atgctcatta cccgcggtga taatctgacc
    18121 ggtggtgtaa tccatcaata cccaggaccc ggcatgaatc tctggaggct gaggtgaaaa
    18181 aggaatgttt tccgccgcaa aaccagacga taaattaaaa acgaacaaag aagcagcaat
    18241 aataagacgg cgtttcaaca gcaaaccctc aggtgtttca aatagctgtt ctttttacgg
    18301 aaatacttat gaactggctg gaataaagtg caagaaaatg tgactactat ctcattttta
    18361 tctgacatga tctgttgcca ctcgctgcca aattgtcgcg ctaaagctga ttagcacggt
    18421 gatatttgat actctggcag acagcagaaa taacggattt aacctaatga tgaatgacgg
    18481 taagcaacaa tctacctttt tgtttcacga ttacgaaacc tttggaacgc accccgcgtt
    18541 agatcgccct gcacagttcg cagccattcg cactgataac gaattcaatg tcatcggcga
    18601 acccgaagtc ttttactgca agcccgcgga tgactattta ccccagcctg gagcagtatt
    18661 aattaccggt attaccccgc aggaggcacg ggcgaaagga gaaaacgaag ccgcgtttgc
    18721 cgcccgtatt cactcgcttt ttaccgtacc gaagacctgt attctgggct acaacaatgt
    18781 gcgtttcgac gacgaagtca cacgcaacgt tttttatcgt aatttctacg atccttacgc
    18841 ctggagctgg cagcatgata actcgcgctg ggatttactg gatgttatgc gtgcctgtta
    18901 tgccctgcgc ccggaaggaa taaactggcc tgaaaatgat gacggtctac cgagctttcg
    18961 ccttgagcat ttaaccaaag cgaatggtat tgaacatagc aacgcccacg atgcgatggc
    19021 tgatgtgtac gccactattg cgatggcgaa actggtaaaa acgcgtcagc cacgcctgtt
    19081 tgattatctc tttacccatc gtaataaaca caaactgatg gcgttgattg atgttccgca
    19141 gatgaaaccc ctggttcacg tttccggaat gtttggggca tggcgtggca ataccagctg
    19201 ggtggcaccg ctggcgtggc atcctgaaaa tcgcaatgcc gtaattatgg tggatttggc
    19261 aggagacatt tcgccattac tggagctgga tagcgacaca ttgcgcgagc gtttatatac
    19321 cgcaaaagcc gatcttggcg ataacgccgc cgttccggtt aagctggtgc atatcaataa
    19381 atgtccggta ctggcccagg cgaatacgct acgcccggaa gatgccgacc gactgggaat
    19441 taatcgtcag cattgcctcg ataacctaaa aattctgcgt gaaaatccgc aagtgcgtga
    19501 aaaagtggtg gcgatattcg cggaagccga accgtttacg ccttcagata acgtggatgc
    19561 acagctttat aatggctttt tcagtgacgc cgatcgtgca gcaatgaaaa ttgtgctgga
    19621 aaccgagccg cgtaatttac cggcactgga tatcactttt gttgataaac gaattgaaaa
    19681 actgttgttc aattatcgag cgcgcaactt cccggggacg ctggattatg ccgagcagca
    19741 acgctggctg gagcaccgcc gccaggtctt cacgccagag tttttgcagg gctacgctga
    19801 tgaattgcag atgctggcac aacagtatgc cgatgacaaa gagaaagtgg cgctgttaaa
    19861 agcgctttgg cagtacgcgg aagagatcgt ctgaaacaaa aaagccggag cgacaaactc
    19921 cggctttttg agatttaccg gatgattatg tcatccggtt tgtaagatta cgctacgtct
    19981 tcgtactgcg gaaccgggtt acggaagctc ttggttacgc aagccaggta aatcagaccg
    20041 atagcagccc agatcagacc cagaaccatt gagctttctt ccaggttaac ccacagcgca
    20101 ccaacggtca gcgcaccaca catcggcagg aacagatact ggaagtgatc tttcagcgtc
    20161 ttgttacgct tctcacggat ccagaactgc gagatgaccg acaggttaac gaaggtgaac
    20221 gccaccaacg caccaaagtt aatcagcgcc gtagccatta ccagatcgaa gttgattgcc
    20281 agcagggcaa tcgcgccaac caggatgatg ttcatcgccg gagtacgcca tttcgggtgt
    20341 acataaccga agaagctttt cgggaacacg ccgtcacgac ccattacgta catcagacgc
    20401 gctacgcctg cgtgcgccgc cataccggac gccagtacgg taatggtgga gaagatcagc
    20461 gcgccaacct ggaacgcttt acccgcaacg tacagcatga tttcaggctg tgacgcatcc
    20521 ggatctttaa agcgagagat atccgggaag tacagctgca ggaagtaagt tgcaaagatg
    20581 aagatcatgc cgccgatcag cgcggtcagg aaaatcgcac gcgggatcac gcgctctgca
    20641 tctttggttt cttccgacag gttgctgatg ccgtcaaaac cggtaaagga gaagcacagg
    20701 attgtcgccc cggtaatcat cgggataaca tgcgcatcgc cagaccagaa cggacgagtg
    20761 ctcgccagcg tacccgcgcc ttcaccttca aatacgccat aaacaaccat gcccagaatc
    20821 accgcgatca gcactacctg caacacgacg attacagtgt tgaagttcgc tacggatttc
    20881 agactacgca ggttaaaggc ggtcataaag gccaccagcg ccaccacaaa catccacgat
    20941 ggaatggaag gcaccagagc ttcaaaatag attttcgcca gcagaatgtt gatcatcggc
    21001 gcgaacagat agtcgagcag agaagaccaa cccaccataa agccgacagt cgggctaatg
    21061 gatttctggg cgtaagtgta tgcagagcca gcagaaggat agcggcgaac cagcttcccg
    21121 tagctcagag ccgtaaacag gatcgcaatc aatgcgaacg cataggctgt cggcacatga
    21181 ccatccgtga ggcctgaaac gataccaaat gtatcaaaca gcgtcatcgg ctgcatatag
    21241 gctagaccca tcattacaac cggaactaac gtaagcgttt tacgcaattc cacgcgagag
    21301 gtgtttggag taacgttatg cgacatggtc attctccttt acggtgataa ccgtcgcgta
    21361 agcaaaaaat tgccccattt ttttggattc ctcagcgaca acaactgtcg atttttagta
    21421 aatatctatc cggtacgaag cccggcctct tggtatgaat gattggtttg aagcaaaaaa
    21481 taaccgacgc taattaaacg tcggttttta gtcatttttt gacagcggcg cattgtgcct
    21541 aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat aataattttt ctttaaatgg
    21601 ctgattttcc gtcatcagat tttgcataaa caccggcgat cgcactattt gctaaaattt
    21661 catctcgcca ccagccgctg gcaagccctg cggtctgttc attccagcca atccagacgg
    21721 gttcaaagag agtttgggcg accactttct tctcgattaa cgcgccacta tcgagaaaac
    21781 gttgcgccag ataccgtggt aaatagccgc agccaaggcc gctaatttgc agctccagtt
    21841 tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc gagaagttgt gaggccgtag
    21901 aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg atatcgttta atgatacgac
    21961 ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg cgcgaccgca aagacttgtt
    22021 caagatcgcc caaccgcgag aaaccaaact cactggaaga tggtggctca tgcatcgcgc
    22081 caacgataat atccgctcgc ccctgagtca gggcatccca ggaaccaccg agtacgccat
    22141 tgataaactt caggcgcgtc acgctatgat gttgataaaa agcttcaatg agaggcgcaa
    22201 gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag ctcgttttcc cagccttcat
    22261 gcagtttgat cgcctgcttt tccagttctc gcacggtatg cagaacttcc cgccctttct
    22321 ctaataacat ttttccggta cgggtgaatt tagcgcggtg tccgctacga tcaagcaatt
    22381 gaatattaag gtcgctttcc agcttgtgaa cggtgtaact cagagcggac ggcgtcttgt
    22441 agagtttggc tgacgccgcc gcaaaactgc cttctttttc taaggcatcg aggatcatca
    22501 acacatccag cagtggtttc atacacgccc ccttgcagtg catgcggcgt cctgctggcg
    22561 tgatcgttta ctccagcggc attaccagcg gatcgggata ctggtattca aatcccagtt
    22621 cattacaaat ccgactgcca tcaataatct tacctttgcc gctgtccaga ctatttctga
    22681 actgcggagg ttccagcccc agtaaacggg ccatctgcgg atagaaaaca ttacgcgcag
    22741 ggtgagctgg cgcacatata ttatagatgt gtccgccttt aggtgcctgt aacaacagag
    22801 tgatagcgcc aataacatct tctaaatgga ctaaattaac accatgttca ccatcagggg
    22861 cggtttttcc ggcaaagaag cgtccgggat gacgtcccgg tccgaccagg cccgcaagac
    22921 gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca gtcttcgagt tcttctaata
    22981 ctcgtccact gttggttacc ggattacgcg gggtggtttc tttcaccgtg ccttgtgcgt
    23041 cgccatagac agacgtcgag ctggtaaaaa taatacgggg aatacgatga gccagcgcgc
    23101 tatccactaa ctcttgtacc gcttgtaaat agaactcatc gccggggccg ctacgacgtg
    23161 ccggaagcgt aatgaccagc gcatcggcat ccatcagggc atccagatcg tcagaatcgc
    23221 aaactaactc aggctccatg cgaagcagat agctatcaat gccactcatt cgggccgctt
    23281 cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca gcctcgcgct gaaagtgaca
    23341 tcgccagcgg catgcctaac caccctaacc cgacaattgc gacctttttc atccgttatc
    23401 tcctgataca ccttagatct ataaggctac gctagcgtat caaaactgac aattcattct
    23461 atgaatgaat ctgttcaata atgataacga catgctgcaa tgagcagtga atcatcagta
    23521 accgcgtata ccagacggtg ctcctctgta atgcgtcggg accagaaacc tgacaaatta
    23581 tgtttcaggg gttctggttt ccccttacct tcaaatggtg ttctgcgggt atctttgata
    23641 agttcattga tctttttaac aattcgctta tctgtttcct gccagtacag ataatcatcc
    23701 catgattcct cagaccagat tagtttcact caataatgtc cttttccgtt cctttgcctg
    23761 atttcaggct atcgattgag tccatcaatc tccgggcgtt agcgggggaa cgcagtagat
    23821 aagccgtctc ttccagcgag ttgtattctt cgagtgacat cagaacacaa gcctctccat
    23881 tctgacgagt aatgaggatc ggggcatgat cttcaacggc tttcatcatt gttgccgaca
    23941 aattctgacg cgcttcgctg tagctaattg tacgcatgta aatctcctct tttgtacagt
    24001 tcattgtaca atgatgggtg ttaattaact atttattaat tagtttgtag atcaagacat
    24061 tgtcagcgag acgaaaaatc agactttgct ctttttggtg ctgtcaagtt agaggacagt
    24121 cctcttagcc cccttctttc cccgctcatt cattaaacaa atccatcgcc ataaaatata
    24181 taaaaaagcc cttgctttct aacgtgaaag tggtttaggt taaaagacat cagttgaata
    24241 aacattcaca gagactttta tgacacgcgt tcaatttaaa caccaccatc atcaccatca
    24301 tcctgactag tctttcaggc gatgtgtgct ggaagacatt cagatcttcc agtggtgcat
    24361 gaacgcatga gaaagccccc ggaagatcat cttccggggg cttttttatt gcgcggttgt
    24421 taacggttca gacaggttta aagaggaata agaaaatgac agacaacact cgtttacgca
    24481 tagctatgca gaaatccggc cgtttaagtg atgactcacg cgaattactg gcgcgctgtg
    24541 gcattaaaat caatcttcac acccagcgcc tgatcgcgat ggcagaaaac atgccgattg
    24601 atattctgcg cgtgcgtgac gatgacattc ccggtctggt aatggacggc gtggtagacc
    24661 ttgggattat cggcgaaaac gtgctggaag aagagctgct taaccgccgc gcccagggtg
    24721 aagatccgcg ctactttacc ctgcgtcgtc tggatttcgg cggctgccgc ctttcgctgg
    24781 caacgccggt tgatgaagcc tgggacggtc cgctctcctt aaacggtaaa cgtatcgcca
    24841 cctcttatcc tcacctgctc aagcgttatc tcgaccagaa aggtatctct tttaaatcct
    24901 gcttactgaa cggttctgtc gaagttgcgc cgcgcgccgg actggcggat gcgatttgcg
    24961 atctggtttc caccggtgcc acgctggaag ctaacggcct gcgcgaagtc gaagttatct
    25021 accgctcgaa agcctgcctg atccaacgcg atggcgaaat ggaagaatcc aaacagcaac
    25081 tgatcgacaa actgctgacg cgtattcagg gcgtgatcca ggcgcgcgaa tcaaaataca
    25141 tcatgatgca cgcaccgacc gaccgactgg atgaagtcat cgccctgctg ccaggtgccg
    25201 aacgcccaac cattctaccg ctggcgggtg atcaacaacg cgtagcgatg cacatggtca
    25261 gcagcgaaac cctgttctgg gaaacgatgg aaaaactgaa agcgctgggt gccagttcaa
    25321 tcctggtcct gccgattgag aagatgatgg agtgatcgcc atgagcttta acacaatcat
    25381 tgactggaat agctgtactg cggagcaaca acgccagctg ttaatgcgcc cggcgatctc
    25441 cgcctctgaa agcattaccc gcaccgttaa cgatattctc gataacgtga aaacgcgtgg
    25501 cgatgaggcc ctgtgtgaat acagcgcgaa gtttgataaa accacggtta ccgcgctgaa
    25561 ggtgtctgct gatgagatca ccgccgccag cgaacgcctg agcgacgagc taaaacaggc
    25621 gatggcggtg gcagtaaaga atattgaaac cttccacact gcgcaaaaac tgccgccggt
    25681 agatgtagaa acgcagccag gcgtgcgttg ccagcaggtc acgcgcccgg tggcttcagt
    25741 gggtttgtat attcctggcg gctccgcccc gctcttctca acggtattaa tgctggcaac
    25801 tccggcgcgt attgcgggct gtaaaaaagt ggtgctgtgc tcaccgccgc cgattgccga
    25861 tgagatcctt tatgcggcgc agctgtgcgg tgtgcaggac gtgtttaacg tcggcggcgc
    25921 acaggccatt gccgcgctgg cgtttggtac ggaatctgtg ccgaaagtgg acaaaatctt
    25981 cgggccgggt aacgcctttg tcaccgaagc aaaacgccag gtaagccagc gtctggacgg
    26041 tgcggcgatc gatatgcccg caggcccgtc ggaagtgttg gtcattgctg acagcggcgc
    26101 aacgccggat ttcgtggctt ctgatttgct ctctcaggct gaacacggcc cggactctca
    26161 ggtgatttta ctgacgcccg atgctgagat ggcgcgtcgc gtagccgaag ctgtcgaacg
    26221 ccaactggca gaactgccgc gtgccgaaac cgcccgtcag gcactgagca ccagccgcct
    26281 gattgtgact aaagatttag cgcagtgcgt aacgatctcc aaccagtacg gcccggagca
    26341 cctgatcatt cagacccgca acgcccgcga gctggtcgat ggcatcacca gcgccggttc
    26401 ggtatttctt ggtgactggt caccggaatc ggcaggcgac tatgcctccg gtaccaacca
    26461 cgtactaccg acttacggtt ataccgcgac gtgttccagc ctcggcctgg ctgatttcca
    26521 gaagcgtatg accgtgcagg agctgtcgaa agaaggtttc tccgcgctgg cgtcgaccat
    26581 tgaaacactg gccgccgccg agcgcctgac tgcccacaaa aatgccgtta ctttgcgtgt
    26641 taacgccctt aaggagcaag catgaacacc gtgactatta ccgatttagc gcgtgaaaac
    26701 gtccgcaacc tgacgccgta tcagtcggcg cgtcgtctgg gcggtaacgg cgacgtctgg
    26761 ctgaacgcca acgaataccc cacagccgtg gagtttcagc tcactcaaca aacgctcaac
    26821 cgctatccgg aatgtcagcc aaaagcggtg attgaaaatt acgcgcaata tgcaggcgta
    26881 aaaccggaac aggtgctggt cagccgtggc gcggacgaag gtattgaact gctgattcgc
    26941 gctttttgcg aaccgggtaa agacgccatc ctctactgcc cgccaacgta cggcatgtac
    27001 agcgtcagcg ctgaaaccat tggcgtcgag tgccgcacag tgccgacgct ggacaactgg
    27061 caactggact tacagggcat ttccgacaag ctggatggcg taaaagtggt ctatgtttgc
    27121 agccccaaca accccaccgg gcaactgatc aacccgcagg attttcgcac cctgctggag
    27181 ttaacccgcg gtaaggcgat tgtggttgcc gatgaagcct atatcgagtt ttgcccgcag
    27241 gcatcgctgg ctggctggct ggcggaatat ccgcacctgg ctattttgcg cacactgtcg
    27301 aaagcttttg ctctggcggg gcttcgttgc ggatttacgc tggcaaacga agaagtcatc
    27361 aacctgctga tgaaagtgat cgccccctac ccgctctcga cgccggttgc cgacattgcc
    27421 gcacaggcgt taagcccgca gggaatcgtc gccatgcgcg aacgggtggc gcaaattatt
    27481 acagaacgcg aatacctgat tgccgcactg aaagagatcc cctgcgtgga gcaggttttc
    27541 gactctgaaa ccaactacat tctggcgcgc tttaaagcct ccagcgcagt gtttaaatct
    27601 ttgtgggatc agggcattat cttacgtgat cagaataaac aaccctcttt aagcggctgc
    27661 ctgcgaatta ccgtcggaac ccgtgaagaa agccagtgcg tcattgacgc cttacgtgcg
    27721 gagcaagttt gatgagtcag aagtatcttt ttatcgatcg cgatggaacc ctgattagcg
    27781 aaccgccgag tgattttcag gtggaccgtt ttgacaaact cgcctttgaa ccgggcgtga
    27841 tcccggagct gctgaagctg caaaaagcgg gctacaagct ggtgatgatc actaatcagg
    27901 atggtcttgg aacacaaagt ttcccgcagg cggatttcga aggcccgcac aacctgatga
    27961 tgcagatctt cacctcgcaa ggcgtacagt ttgatgaagt gctgatttgt ccgcacctgc
    28021 ccgccgatga gtgcgactgc cgtaagccga aagtaaaact ggtggagcgt tatctggctg
    28081 agcaagcgat ggatcgcgcc aacagttatg tgattggcga tcgcgcgacc gacattcaac
    28141 tggcggaaaa catgggcatt aatggtttac gctacgaccg cgaagccctg aactggccga
    28201 tgattggcga acaactcacc aaacgagacc gttacgccca tgtagtgcgc aacaccaaag
    28261 agacgcagat tgacgtccag gtgtggctgg atcgcgaggg tggcagcaag attaacaccg
    28321 gcgttggctt ctttgatcac atgctggatc agatcgccac ccacggcggt ttccgtatgg
    28381 aaatcaacgt taaaggcgac ctctatatcg acgatcatca caccgtcgaa gataccggcc
    28441 tggcgctggg cgaagcgtta aaaattgccc ttggcgataa acgcggtatt tgccgctttg
    28501 gttttgtgct gccgatggac gaatgccttg cccgctgcgc gctggatatc tctggtcgcc
    28561 cgcacctgga atataaagct gaatttacct accagcgcgt gggcgatctc agcaccgaga
    28621 tgatcgagca cttcttccgt tcgctttcat acaccatggg cgtgacgcta cacctgaaaa
    28681 ccaaaggtaa aaacgatcac caccgtgtag agagcctgtt caaagccttt ggtcgtaccc
    28741 tgcgccaggc catccgcgtc gaaggcgaca ccctgccctc gtcgaaagga gtgctgtaat
    28801 gaacgtggtg atccttgata ctggctgcgc caacctgaac tctgtgaagt ctgccattgc
    28861 gcgccacggt tatgaaccca aagtcagccg tgacccggac gtcgtgttgc tggccgataa
    28921 actgttttta cccggcgttg gcaccgcaca agcagcgatg gaccaggtgc gtgagcgcga
    28981 gctatttgat ctcatcaaag cctgtaccca accggtgctg ggcatctgct tagggatgca
    29041 actgctcggg cgacgtagcg aagagagcaa tggcgtcgac ttgctgggca tcatcgacga
    29101 agacgtgccg agaatgaccg actttggtct gccactgcca catatgggct ggaaccgcgt
    29161 ttatccgcag gcaggcaacc gcctgtttca ggggattgaa gacggcgcgt acttttactt
    29221 tgttcacagc tacgcaatgc cggtcaatcc gtggaccatc gcccagtgta attacggcga
    29281 accgttcacc gcggcggtac aaaaagataa cttctacggc gtgcagttcc acccggagcg
    29341 ttctggtgcc gctggcgcta agttgctgaa aaacttcctg gagatgtgat gattattccg
    29401 gcattagatt taatcgacgg caccgtggtg cgtctccatc agggcgatta cggcaaacaa
    29461 cgcgattacg gtaacgaccc gctgccacgc ttacaggatt acgctgcgca aggtgccgaa
    29521 gtgttgcatc tggtggatct gaccggggca aaagatccgg ctaagcgtca aatcccgctg
    29581 attaaaaccc tggtcgcggg cgttaacgtc ccggtgcagg ttggcggcgg tgtgcgtagt
    29641 gaagaagacg tagcgacgtt actggaagcg ggcgttgcac gcgtggtggt tggctccacc
    29701 gcggtgaaat caccagaaat ggtgaaaggc tggtttgaac gttttggtgc tgatgcctta
    29761 gtactggcgc tggatgtccg tattgacgag caaggcaaca agcaggtggc ggtcagcggc
    29821 tggcaaaaga actcgggcgt ttcactggaa caactggtgg aaacctatct tcccgtcggc
    29881 ctgaaacatg tgctatgtac cgatatctcg cgcgacggca cgctggcagg ttctaacgtc
    29941 tctttatatg aagaagtgtg cgccagatac ccgcaggtgg catttcagtc ctccggcggc
    30001 attggcgaca ttaacgatgt tgctgccctg cgaggtactg gcgtgcgcgg cgtaatagtt
    30061 ggtcgggcat tactggaagg taaattcacc gtgaaggagg ccatcgcatg ctggcaaaac
    30121 gcataatccc ttgtctcgac gttcgtgatg gtcaggtggt gaaaggcgta cagtttcgca
    30181 accatgaaat cattggcgat atcgtgccac tggcaaaacg ctacgctgaa gaaggtgcag
    30241 acgaactggt gttctacgat atcaccgctt ccagcgatgg ccgtgtggta gataaaagct
    30301 gggtatctcg cgtggcggaa gtcatcgaca ttccattctg cgtggcgggc gggattaaat
    30361 cgctggatga tgccgcgaaa attctttcct ttggcgcgga taaaatttcc atcaactctc
    30421 ctgcgctggc ggacccgacg ttaattactc gcctggcgga tcgctttggt gtgcagtgta
    30481 ttgtggtcgg tattgatacc tggtacgacg ccgaaaccgg taaatatcat gtgaatcaat
    30541 ataccggcga tgaaagccgc acccgcgtca ctcagtggga aacactcgac tgggtacagg
    30601 aagtgcaaaa acgtggagcc ggagaaatcg tcctcaacat gatgaatcag gacggcgtgc
    30661 gtaacggtta cgacctcgaa cagctgaaaa aagtgcgtga agtttgccac gtcccgctga
    30721 ttgcctccgg tggcgcaggc accatggaac actttctcga agccttccgc gatgccgacg
    30781 ttgacggcgc gctggcagct tccgtattcc acaaacaaat aataaatatt ggtgaattaa
    30841 aagcgtacct ggcaacacag ggcgtggaga tcaggatatg ttaacagaac aacaacgtcg
    30901 cgaactggac tgggaaaaaa ccgacggact gatgccggtg attgtgcaac acgcggtatc
    30961 cggcgaagtg ctaatgctgg gctatatgaa cccggaagct ctggacaaaa ccatcgaaag
    31021 cggcaaagtc accttcttct cgcgcactaa acagcgactg tggaccaaag gcgaaacgtc
    31081 gggtaatttc ctcaacgtag taaatattgc gccggactgc gacaacgaca cgttactggt
    31141 gctggcgaat cccatcgggc caacctgcca caaaggcacc agcagctgct tcggcgacac
    31201 tgctcaccag tggctgttcc tgtatcaact ggaacaactg ctcgccgaac gtaaatctgc
    31261 cgacccggaa acctcctaca ccgccaaact gtatgccagc ggcactaaac gcattgcgca
    31321 gaaagtgggc gaagaaggcg tggaaaccgc actggcagca acggtacatg atcgctttga
    31381 gctgaccaac gaggcatctg acttgatgta tcacctgctg gtgctgttgc aggatcaggg
    31441 gctggatctc ggagaagtga ttgataatct gaagaatcgg cattaacgcg ttggcccccg
    31501 cagcaacggc gggggtagca gctctcagga tttgctttcc agcaatgtag tgtagagatt
    31561 gacataggcg tcaatcatat ggtcggcagt aaacatctct tcaaaacgac gacgcgagtt
    31621 ttcgccatag cggttggagg tttcctcgtt attccagagc tcattcatcg cctccaccag
    31681 cgcctggcta tcattcggcg gaatcacgca accactcact ttgtcctggt taatgaaaga
    31741 ggtacctgta ccgatctcgc aagagatcag cggccttgca aagcgtgcgc cttccaataa
    31801 cgtaatgcca aacgcctcag agcgcagatg cgaggggaat accacgcccc ggcagagctg
    31861 gaagagaatg tacttatctt cgtcgttgag catgccggta aacatcacat tgctcagccc
    31921 gcgctgctgc gcttcacgcc gcacttccga ttccagcggc ccgcccccta caaccaccac
    31981 aggcagtcgg ctacgctcag cggcatccat cagaatatgc agccctttgt agtagcggaa
    32041 agtgccgacg aagagaaaga acttatcgcc gacagtttcc cgccagtgcg cgaccctctg
    32101 cggatcgtgc tgcacgtcct gctgctccag accaaacggg atcaccaccg ttttatccag
    32161 atattttttc agggtccggc tggaagccac gtaattgggc gacgaggcaa cgatgcaatc
    32221 tacgccgctg agaaatcgct cctgcagcgg ctggtacagc ttcattaacc gtttttgttt
    32281 cactatatca gagtgatagg tcaccacagt cctggcgtca ggccgcgccg aaagatgcag
    32341 catatccata aagggaaacg gaaaatggta gttgatgata tcgtagtcat ctttaatcag
    32401 cttaaaacgc tttaacgctt tcagcgaaaa cggcgtggag gcaatttcaa aaagctgttt
    32461 attataaatg acccgatgat cttcgtaagc gacaggacct gtatctttgt ccgggctaaa
    32521 agtgaaaaca tcggctgcga ttccccggcg ggcgcagccc tgacttagct gataaatgac
    32581 ctgctcaata ccgccgtagg tatcgggata gtaagtctta tagacgtgta gaactctcaa
    32641 ctgacaccct tagagtaatt tataggcgtt aatggtctgg gtcgcacagt tctcccacga
    32701 aaactgtttc gcctgagcaa gaccgcgcgc ggtggcgatg tcccgccagc tatcatcctg
    32761 caggctctgc aaaatttgcg cgctaatcgc gtctatatca ttaggatcgg caacgaggcc
    32821 ggcatcgcca acaacctcag gcaaagaggt gacattggag catactaccg gcacaccgca
    32881 agacatcgct tcaagaatag gtaaaccaaa tccctcgtag aaggagggat aaacaaagac
    32941 tctggccgct gcgtacaaat acggcaggtc ttcatccgga acatatccga ggtaacggat
    33001 ccagccttcc cgagtaccgc gctcgactaa ctgccacagc acatcgtctt cccagccgcg
    33061 ataaccgcta aggattagcg gatagcgcat gcggatctcc atcggtagca gctggtaggc
    33121 atgtagcagg cctcggatat ttttgcgtgg ctccatggtg ccgatatata gcgcgtaggc
    33181 ctgccacgcc agctgatatt tctgcagtac cggcagacac tctgccgggc tgcgtgggat
    33241 atagtcactg ctgcaggcta gcttggtggt tacgatccgc tccgccggat agttgaacaa
    33301 gcggataatt tcactgcgcg agaaatcaga aacagtcagg atcagctttg ccgaatccag
    33361 actctcatgt agggacttct ccatatagcg aacccgatct tttggatgat attccgggca
    33421 ggtaaaaatg gatatgtcat gaaacgtggt cacggcgcgt tccagtttat gcggcagata
    33481 aaaattgggg ccatggtaga tataatcttt atagtcgcgc aatgcccacg cctggcgccg
    33541 cggatgcagc aagcgataag cctcaatcaa cagcgtctgt cggcgtagaa acgccgacag
    33601 acgaccatga ttgctggctt tggtatcgct tttattctcc accaaaggga tctgttcgat
    33661 aaacgacgca ccgtgaaata gctttaattc ttcaatttcg cgggcgaccg ccagccgctt
    33721 aaccagctcc agggaatacc gaccgatgcc cgttaatggg tatttaattg gctcagtagc
    33781 aaa
//
LOCUS       DADSZD010000032        33185 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.032, whole genome shotgun sequence.
ACCESSION   DADSZD010000032 DADSZD010000000
VERSION     DADSZD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.032"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            427..1494
                     /locus_tag="J1Z28_001402"
                     /pseudo
     CDS             427..1494
                     /locus_tag="J1Z28_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
     gene            1491..4226
                     /gene="rbbA"
                     /locus_tag="J1Z28_001403"
     CDS             1491..4226
                     /gene="rbbA"
                     /locus_tag="J1Z28_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBA7716249.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGSVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGKVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGIPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4226..5359
                     /locus_tag="J1Z28_001404"
     CDS             4226..5359
                     /locus_tag="J1Z28_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7716250.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSI
                     GIFMGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLA
                     QAILYRGAGFEIVWPQFLTLIAIGGAFFTIALLRFRKTIGTMA"
     gene            5432..5707
                     /locus_tag="J1Z28_001405"
     CDS             5432..5707
                     /locus_tag="J1Z28_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBA7716251.1"
                     /translation="MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            5704..6063
                     /locus_tag="J1Z28_001406"
     CDS             5704..6063
                     /locus_tag="J1Z28_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBA7716252.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6113..6973)
                     /locus_tag="J1Z28_001407"
     CDS             complement(6113..6973)
                     /locus_tag="J1Z28_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HBA7716253.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLIRKAS"
     gene            complement(7005..7274)
                     /locus_tag="J1Z28_001408"
     CDS             complement(7005..7274)
                     /locus_tag="J1Z28_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBA7716254.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFSEAE"
     gene            complement(7264..8772)
                     /locus_tag="J1Z28_001409"
     CDS             complement(7264..8772)
                     /locus_tag="J1Z28_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBA7716255.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQSVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAVSLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWESWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(8765..10123)
                     /locus_tag="J1Z28_001410"
     CDS             complement(8765..10123)
                     /locus_tag="J1Z28_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA7716256.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALFFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(10200..10481)
                     /locus_tag="J1Z28_001411"
     CDS             complement(10200..10481)
                     /locus_tag="J1Z28_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA7716257.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(10478..10951)
                     /locus_tag="J1Z28_001412"
     CDS             complement(10478..10951)
                     /locus_tag="J1Z28_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7716258.1"
                     /translation="MQGIQFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMSGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMRALLTMETTEIHTVILNKMKERGEI"
     gene            complement(10976..11722)
                     /locus_tag="J1Z28_001413"
     CDS             complement(10976..11722)
                     /locus_tag="J1Z28_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7716259.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSKRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRELPVEFGNVWLKGNKYYLGTVLQRRELS"
     gene            complement(11921..12322)
                     /gene="nikR"
                     /locus_tag="J1Z28_001414"
     CDS             complement(11921..12322)
                     /gene="nikR"
                     /locus_tag="J1Z28_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBA7716260.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(12328..13134)
                     /gene="nikE"
                     /locus_tag="J1Z28_001415"
     CDS             complement(12328..13134)
                     /gene="nikE"
                     /locus_tag="J1Z28_001415"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBA7716261.1"
                     /translation="MTLLNVSDLSHHYAHGGFSGKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(13131..13895)
                     /gene="nikD"
                     /locus_tag="J1Z28_001416"
     CDS             complement(13131..13895)
                     /gene="nikD"
                     /locus_tag="J1Z28_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBA7716262.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(13895..14728)
                     /gene="nikC"
                     /locus_tag="J1Z28_001417"
     CDS             complement(13895..14728)
                     /gene="nikC"
                     /locus_tag="J1Z28_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBA7716263.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(14725..15669)
                     /gene="nikB"
                     /locus_tag="J1Z28_001418"
     CDS             complement(14725..15669)
                     /gene="nikB"
                     /locus_tag="J1Z28_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBA7716264.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSITFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(15669..17243)
                     /gene="nikA"
                     /locus_tag="J1Z28_001419"
     CDS             complement(15669..17243)
                     /gene="nikA"
                     /locus_tag="J1Z28_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBA7716265.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(17354..17941)
                     /gene="acpT"
                     /locus_tag="J1Z28_001420"
     CDS             complement(17354..17941)
                     /gene="acpT"
                     /locus_tag="J1Z28_001420"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBA7716266.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(17996..19045)
                     /locus_tag="J1Z28_001421"
     CDS             complement(17996..19045)
                     /locus_tag="J1Z28_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA7716267.1"
                     /translation="METPQPDKTGMRILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            19144..20394
                     /locus_tag="J1Z28_001422"
     CDS             19144..20394
                     /locus_tag="J1Z28_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7716268.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFMSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(20398..20955)
                     /gene="dcrB"
                     /locus_tag="J1Z28_001423"
     CDS             complement(20398..20955)
                     /gene="dcrB"
                     /locus_tag="J1Z28_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBA7716269.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(21028..21693)
                     /locus_tag="J1Z28_001424"
     CDS             complement(21028..21693)
                     /locus_tag="J1Z28_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBA7716270.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFDALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            21914..22159
                     /gene="tusA"
                     /locus_tag="J1Z28_001425"
     CDS             21914..22159
                     /gene="tusA"
                     /locus_tag="J1Z28_001425"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBA7716271.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(22420..24618)
                     /gene="zntA"
                     /locus_tag="J1Z28_001426"
     CDS             complement(22420..24618)
                     /gene="zntA"
                     /locus_tag="J1Z28_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBA7716272.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGTTSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGVFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(24692..25318)
                     /locus_tag="J1Z28_001427"
     CDS             complement(24692..25318)
                     /locus_tag="J1Z28_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBA7716273.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            25459..25818
                     /locus_tag="J1Z28_001428"
     CDS             25459..25818
                     /locus_tag="J1Z28_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBA7716274.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(25821..26090)
                     /locus_tag="J1Z28_001429"
     CDS             complement(25821..26090)
                     /locus_tag="J1Z28_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBA7716275.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(26080..26676)
                     /gene="rsmD"
                     /locus_tag="J1Z28_001430"
     CDS             complement(26080..26676)
                     /gene="rsmD"
                     /locus_tag="J1Z28_001430"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBA7716276.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            26826..28352
                     /gene="ftsY"
                     /locus_tag="J1Z28_001431"
     CDS             26826..28352
                     /gene="ftsY"
                     /locus_tag="J1Z28_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBA7716277.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVPAQEP
                     AKASEQTVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAVSPEEWQAEAETVEIVEAAEEEAAKEEI
                     TDEEPEAQALAAEVAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTK
                     ENLGSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAE
                     ALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKS
                     VMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIA
                     DTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVG
                     LTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED
                     "
     gene            28355..29023
                     /gene="ftsE"
                     /locus_tag="J1Z28_001432"
     CDS             28355..29023
                     /gene="ftsE"
                     /locus_tag="J1Z28_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBA7716278.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            29016..30074
                     /gene="ftsX"
                     /locus_tag="J1Z28_001433"
     CDS             29016..30074
                     /gene="ftsX"
                     /locus_tag="J1Z28_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBA7716279.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            30319..31173
                     /gene="rpoH"
                     /locus_tag="J1Z28_001434"
     CDS             30319..31173
                     /gene="rpoH"
                     /locus_tag="J1Z28_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBA7716280.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            31445..32548
                     /locus_tag="J1Z28_001435"
     CDS             31445..32548
                     /locus_tag="J1Z28_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA7716281.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLSWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32736..33119)
                     /gene="panM"
                     /locus_tag="J1Z28_001436"
     CDS             complement(32736..33119)
                     /gene="panM"
                     /locus_tag="J1Z28_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBA7716282.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLKEVLRNNPDVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKR"
BASE COUNT         7927 a         8606 c         9041 g         7611 t
ORIGIN      
        1 cggtatttat tcgcacatca ggcgataata acgctatgtg atagtcatca ggtttccctg
       61 ttacggaaac ctgtgcaaca gaatctgtga acctgtacag aatatttttg gaaaaaacac
      121 cagcgaacgg aagaacatcg cgatgtatca caagttgatg gcaatcaaat agacaacatg
      181 aatataactg ttagtattta attgatttca cttatgagta tgcgtgtttc atttacggcc
      241 aggaatgctg atgatgacaa cattactacc cgttttcact aagccttccc cattggcgct
      301 caatgctctg tgcgctggtc gtatttgcca tttccttctt atcccggatg gaagaatccg
      361 ctgaacatcg ttcggtggca gttgtaacac gcgtcgattg cttacaggga ttatggcgga
      421 tcgattatgg ataagagtaa gcgccatctg gcgtggtggg gtgtcggggc actggcggtg
      481 gctgccgtcg tggcgtggtt gctgttgcgc ccggcgggtg tgccggaagg ctttgctgtc
      541 agcaatgggc gcattgaagc gacggaagtg gatatcgcca gcaaaattgc cgggcgtatc
      601 gacaccattc tggtgaaaga aggccagttt gtttgagaag gtgaagtgct ggcgaagatg
      661 gatactcgcg tgttgcagga acagcgactg gaagccatcg cgcaaatcaa agaagcacaa
      721 agtgccgttg ccgccgcgca ggctttgctg gagcaacgcc aaagcgaaac tcgtgccgca
      781 cagtcgctgg ttaatcaacg ccaggcagaa ctggactccg tagcaaaacg tcatacgcgt
      841 tcccgctcac tggcacaacg gggggcaatt tctgcgcaac agctggatga cgaccgcgcc
      901 gccgctgaga gcgcccgagc tgcgctggaa tcggcaaaag ctcaggtttc tgcttctaaa
      961 gccgctatag aggcggcacg caccaatatc attcaggcgc aaactcgcgt cgaagcggca
     1021 caagccactg aacggcgcat tgccgcagat atcgatgaca gcgaactgaa agccccgcgt
     1081 gacggacgcg tgcagtatcg ggttgccgag ccaggcgaag tgctggcggc aggcggtcgg
     1141 gtgctgaata tggtcgatct cagcgacgtc tatatgacct tctttctgcc aaccgaacag
     1201 gcgggcacgc tgaaactggg cggtgaagca cgtttgattc ttgatgctgc accagatctg
     1261 cgtattcccg cgaccatcag ttttgtcgcc agcgtcgccc agttcacgcc aaaaaccgtc
     1321 gaaaccagcg atgagcggct gaaattgatg ttccgtgtca aagcgcgtat cccaccggaa
     1381 ttactccagc agcatctgga atatgtcaaa accggattgc cgggcgtagc gtgggtgcgg
     1441 gtgaatgaag aacttccgtg gcctgacgac ctggtggtga ggttgccgca atgacgcatc
     1501 tggaactggt tcccgtcccg cctgtcgcgc aactggcggg cgtgagccag cattatggaa
     1561 aaaccgttgc gctgaacaat atcacactcg atattccggc ccgttgcatg gtcgggctga
     1621 ttggaccgga tggtgtcggt aaatcgagct tgttgtcgtt gatttccggt gcccgcgtca
     1681 ttgagcaggg cagcgtgatg gtgctgggcg gcgatatgcg cgacccgaag catcgccgcg
     1741 acgtctgccc gcgcatcgcc tggatgccgc aggggctggg caaaaatctc taccacacct
     1801 tgtcggtgta tgaaaacgtc gattttttcg cccgcctgtt cggtcacgac aaagcggagc
     1861 gggaagtgcg tattaatgag ctgctgacca gcaccggatt agcaccgttt cgcgatcgtc
     1921 cggcagggaa actctccggc gggatgaagc aaaaacttgg gctgtgctgt gcgttaatcc
     1981 acgacccgga attgttgatt cttgatgagc caacaacggg ggttgacccg ctctctcgcg
     2041 cccagttctg ggatttgatc gacagtattc gccagcggca gagcaatatg agcgtgctgg
     2101 tcgccaccgc ctacatggaa gaggccgaac gcttcgactg gctggtagcg atgaatgccg
     2161 gaaaagtgct ggcaactggc agcgccgaag agctgcgaca gcaaacgcaa agcgccacgc
     2221 tggaagaagc atttattaat ctgttaccgc aagcgcaacg ccaggcgcat caggcggtag
     2281 tgatcccacc gtatcaacct gaaaacgcag agattgccat cgaagcgcgc gatctgacca
     2341 tgcgttttgg ttccttcgtt gccgttgatc acgtcaattt ccgcattcca cgcggggaga
     2401 tttttggttt tcttggttcg aatggctgcg gtaaatccac caccatgaaa atgcttaccg
     2461 gattgctgcc cgccagcgaa ggtgaggcgt ggctgttcgg gcagccggtt gatccaaaag
     2521 atatcgatac ccgccgtcgt gtgggctata tgtcgcaggc gttttcgctc tataacgaac
     2581 tcaccgtgcg gcaaaacctt gagttacatg cccgtttatt tcacatcccg gaagcggaaa
     2641 ttcccgcaag agtggctgaa atgagcgagc gttttaagct caacgacgtt gaagatgttc
     2701 tgccggagtc attgccgctc ggcattcgcc agcggctttc gctggcggtg gcggtgattc
     2761 atcgcccgga gatgttaatc ctcgatgagc ctacttctgg cgtcgatccg gtagcgaggg
     2821 atatgttctg gcagttgatg gtggatctct cgcgccagga caaagtgact atcttcatct
     2881 ccacccactt tatgaacgaa gcggaacgtt gtgaccgtat ctcactgatg cacgctggaa
     2941 aagtgctcgc cagcggtata ccgcaggagt tggttgagaa acgcggagcc gccagtctcg
     3001 aagaggcatt tatcgcttat ttgcaggaag cggcagggca gagcaacgaa gccgaagcgc
     3061 cgcctgtggt acatgacact acccacgcgc cacgtcaggg attcagcctg cgccgtctgt
     3121 ttagctacag ccgccgcgaa gcgctggaac tgcgacgcga tccggtacgt tcgacgctgg
     3181 cgctgatggg aacggtgatc ctgatgctga taatgggtta tggcatcagt atggatgtgg
     3241 aaaacctgcg ctttgcggtg cttgaccgcg atcaaaccgt cagtagccag gcgtggacgc
     3301 tcaatctctc cggttcccgt tactttatcg aacagccgcc gctcaccagt tatgacgagc
     3361 ttgatcgtcg gatgcgtgcg ggcgatatca cggtggcaat tgagatccct cctaatttcg
     3421 ggcgcgatat cgcgcgtggt acgcccgtgg aactcggtgt ctggatcgac ggtgcgatgc
     3481 cgagccgcgc cgaaacggta aaaggctacg tgcaggccat gcaccagagc tggctacagg
     3541 atgtggcgag ccgacagtcc acacccgcca gccaaagcgg gctgatgaat attgagacgc
     3601 gctatcgcta taacccggac gtaaaaagcc tgccggcgat tgttccggcg gtgatcccgc
     3661 ttctgctgat gatgatcccg tcaatgctaa gcgcccttag cgtggtgcgg gaaaaagagc
     3721 tagggtcgat tatcaacctt tacgtgaccc ccaccacccg tagcgaattt ttacttggca
     3781 aacagctgcc atacatcgcg ctggggatgc tgaacttttt cctgctctgc gccctgtcgg
     3841 tgtttgtgtt tggcgtaccg cataaaggca gtttcctgac tctcaccctg gcggcgctgc
     3901 tgtatatcat catcgccacc ggaatggggc tgctgatctc cacctttatg aaaagccaga
     3961 ttgccgccat tttcggtacg gcaattatca cgttgatccc ggcgacacag ttttccggga
     4021 tgatcgatcc ggtagcttcg ctggaaggtc caggacgttg gatcggcgag gtttacccga
     4081 ccagtcattt tctgactatc gcccgcggaa cgttctcgaa agcgctggat ctgactgatt
     4141 tgtggcaact ttttatcccg ttgctgatag ccataccgct ggtgatgggc ttaagcatcc
     4201 tgctgctgaa aaaacaggag ggatgatgcg ccatttacgc aatattttta atctgggtat
     4261 caaagagttg cgcagtctgc tcggtgataa agcgatgctg acgctgattg tcttctcgtt
     4321 tacggtgtcg gtgtattcgt cagcgaccgt tacgccagga tcgttgaacc tcgcgccgat
     4381 cgccattgcc gatatggatc aatcgcagtt atcgaaccgg atcgttaaca gcttctatcg
     4441 cccgtggttt ttgccaccgg agatgatcac cgccgatgag atggatgccg ggctggacgc
     4501 cggacgttat accttcgcga taaatattcc gcctaatttt cagcgtgatg tcctcgccgg
     4561 acgccagccg gatattcagg tgaacgttga tgccacgcgc atgagccagg catttaccgg
     4621 caatgggtat atccagaata ttatcaacgg tgaagtgaac agctttgtcg cgcgctaccg
     4681 tgataacagc gaaccgttgg tatcgctgga aacacggatg cgctttaacc cgaaccttga
     4741 tcccgcgtgg tttggcgggg tgatggcgat catcaacaac attaccatgc tggcgattgt
     4801 attgaccgga tcggcgctga tccgcgagcg tgaacacggc acggtggaac acttactggt
     4861 gatgccgata acgccgtttg agatcatgat ggcaaaggtc tggtcgatgg ggctggtggt
     4921 gctggtggtg ctggtggtgt cgggattatc gctggtgctg atggtgaaag gcgtgctggg
     4981 cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc gtggcgctca gtttgtttgc
     5041 caccacgtca atcggcattt ttatggggac gatagcgcgt tcaatgccgc aactggggct
     5101 gctggtgatt ctggtgctgc tgccgctgca aatgctttcc ggtggttcca cgccgcgcga
     5161 aagtatgccg cagatggtgc aggacattat gctgaccatg ccgacgacac actttgttag
     5221 cctcgcgcag gccatcctct accgtggtgc cggattcgaa atcgtctggc cgcagttcct
     5281 gacgctgata gcgattggcg gcgcgttttt taccattgcg ctgctgcgat tcaggaagac
     5341 gattgggaca atggcgtaag gaagggaaac ttgacttcat attgtgatgt catactattg
     5401 atgtcataaa gtgaagtcat aaggacatgc catgagaacc caggtacagg ctttacggaa
     5461 gaaacaaaaa aatacattgg atcaaatatt taaatctcct gttcctcaag ggattaagtg
     5521 gtctgatata gagtcactgg ttaaagcatt aggcggagaa attaaggaag gaagaggttc
     5581 gcgttgtaag ttcatactaa atatgagcgt tgcgtgtttc catcggcctc atccgtcgcc
     5641 agataccgat aaaggcgctg tagaaagcgt gcgtgactgg ttactaagta taggggtaaa
     5701 accatgatca aactgaagac gccaaactct atggagattg ccgggcaacc tgctgttatc
     5761 acttacgtgc cagaactgaa tgcatttcgt ggcaaattcc tgggcttgtc aggttattgt
     5821 gattttgtct ctgacagcat tcaggggctg caaaaagaag gggagctttc cctgcgagag
     5881 tatcttgagg attgtaaagc tgcgggtatt gagccgtatg cgcgtacaga aaagattaag
     5941 acctttacat tacgctatcc agaatcgctg agtgaacgcc ttaataatgc ggctgcgcag
     6001 caacaagtct ctgtaaatac ttatattatt gaaacgctaa atgagcgttt gaatcattta
     6061 taacttatag cggcccaaca atttctgttg cgccgctata tttttgtggc tcttaagatg
     6121 cctttctaat caatcgatgc ccctcattca tcatcaggat tttgctatac acatcttcct
     6181 cgacggcatt ttttgctgat gccatcaccc gtgccagatt cgcctcgttg tgattattca
     6241 catacgcttt gccgactgcg gtgataaacg ctttgcgcag atcgctggcg atattcactt
     6301 tagcgacgcg ataattaacg aaactgcgta atatctccgg ggcgataccg gagccgccgt
     6361 gaataaccag cggcaccggg ctgacttcgg caatgcgctt cagcaggggg atatcgatcc
     6421 gcggtatatc gtccaggcca tgaacgttac caatagaaac tgccagcatg tcgcaaccgg
     6481 tgcgttcgac aaaggtcttc accttttccg gttccgtttt gcaatcggct tcgctgacgt
     6541 gatcatcttc tttgccgaga atcgccccca gctccgcttc aaccggcacg ccataggatt
     6601 tacagaaatc gacagcttcc tgggtgaaag cgatattctc ttcaaaagag aatgcggcac
     6661 cgtcaatcat gaccgaggta aagcccgcgc gcaccgcctg tttaacatcc tccagggttt
     6721 tcccgtgatc cagatgaaga ctgaccggaa catccatttt atcggcgtga cgtttcacga
     6781 tttcatatat gtaatcgtat cccgacagtt gtgcattggt gggcgcaatc tgaatgaagt
     6841 tgggtaatcc cgaacgctca atagcgttta aaattgaaat tgtcgtctcc agattggtgg
     6901 tattaaacgc gcccgctaat acccgttttt cctgaatacg gtcgagcagt atccgtccat
     6961 taactaatgg catattgaaa acctcatttt ttaatatcag ttaattattc agcttcactg
     7021 aagcggtcgc gaaataaggc ggtgagagtc tgtatggcct cgttttcatc atcaccgtcg
     7081 cagcgtattt ccaccgtttt tccgcctttt attccggcgc gcataatatt catcatactg
     7141 gtggcattaa tatcattacc ctcataatgc agcgtcactt tacagctata ctttttggcg
     7201 gctttcgcca gacaggccgc cggacgtgca tgcaggccag tactgtttct gacctccacg
     7261 gttttagtca acatagtgct cctcccgatg atgtaatggc atcacgcctt cttgctgtaa
     7321 tttcaactga taccagcttt cccatttgtg ctgataacgc tgtaattgat gatatcttgc
     7381 ccgattcggt aacaaacgtt gggctatggt aacttgcggc ggcggaatat ccggtgccgc
     7441 ggttaataag gctccgacag aaaccgcctc gctaaatgca attgcttcaa ccggacgctg
     7501 taaaatatct gcctttaatt gcagccaaag ggggttatgg gtcgccggac cgatcacttt
     7561 taacacgctg gtgccaggca cattaaaaca ggcaaagcag ttggcaaatt ccatggttaa
     7621 tcccagtgag acgctggaaa ttaacatgtc gatatttaac gtatcgccaa gcccataaaa
     7681 taatccgcgg gtgtggcgat ttttataggg tgaacccgaa ccgcgaagat gcggaataaa
     7741 gacaggaatg tcatcaagcg accagttccc cgccagataa tccgcatacc cacgattgag
     7801 cgacacggcg atctcctcat cggttagccg gaacgtgtta cgcagccact ccagcgcgaa
     7861 accgcccacg ggcagagaag caaacagggt gaactggttg gcaagcgagt aacagccgtt
     7921 tgccagcttg tcacgtttgg cctgttcatc cagcgtcggt tgtgtatcca gttgcagaat
     7981 gccttccgtg gtgcccgtcg agttaaggat atcgcccggc atcatctgca acgcccgcgc
     8041 acccaccata tggtcatgtc cggccagcgt cacgctgacc gattgcgaga acccaagcgt
     8101 tttacagagc gactcactga cccatccggc gtgttcgcct ggctgcacca atggcgcaaa
     8161 tgctgaacag ggaacgtgta acaacgccgc cgctttcgct gaccattcgc ctttgcgcac
     8221 gtccaggcac agtgtacggc tggctaaggt gatatccgtt cgctgttcgc cagtcagccg
     8281 ccagagcagc acttccggtg catgtagcca gcgcaggccg cgacgattgc gcagcggata
     8341 atgctccagc aaccaacgca ttttgaaggc agagtaattg ctgtgcagtg gtaacccgca
     8401 aatgtcataa agtgccgcac tgtctgcctc gctaagcgtt gccagatact cttcaccgcg
     8461 acggtcatac catgccagca ttggcgtcag gatctcgcca tgttcgtcaa gaaacacccc
     8521 tgactcgcca aaactggcaa tgcttatccg cctgaccggc agcggcgaag cggcgttcag
     8581 ttgcgctatc gcctggcgga cctcctgcca gagggcgtcg atatcgaaat cgacatcgcc
     8641 ctgtggggag atctgctttg ccgtcacgaa tttatgcgcg cccaacgtcg ttgcgtccag
     8701 gcaggaaaag caggtgactt tgcaattggt ggtgccaata tcgataacga tagctgcgct
     8761 gttatcaggc ataaaccgtg ctccgcgtca ttcggaaaat gccatacagt accagggcga
     8821 taaccggcac cagaccaatg gcgatgatat tgccgttgaa gatttccagc agccagaagc
     8881 ggaacgggtt accgccatca aggaaactgg aaacctggcc gctggagcct tcggcaaatt
     8941 tgaagttaac gtctttcgcc atgccggtaa taaacggtgc gattttagtc gcaatccaca
     9001 ggtcggcaat aatcaccggg atcgccgcca gaacggtgcg gaaaatattg ccacggctgg
     9061 ctaaagcaat catcgacgcc attaccgcca ggttggcaag gtcaccaagc ggcaggactt
     9121 cattacctgg gaggacaaac gcgataaaga ccgagattgg cgtcagcagc agggcggtgg
     9181 agataatcgc cgggttacct acggcaacgg cgatatccag gccgatatag agatcgtcac
     9241 ggtcagggta tttggcattg agatactttt taatggcttc agacagcggt aataaacctt
     9301 ccatcaggat gcgtaccatg cgtggcagga tgaacatcac accgccaatg ctgatgccaa
     9361 gcagcaagac ttttttgaaa tcgtatccgg caattacgcc gagcagaatg cccagaatag
     9421 tgccaaccat catcggttcg ccgaagatgc caaacttttt ctgtacggtt tccgggtcga
     9481 tatgaatacg attgaggccg gggatatggt cgatgatttt gtcgaccaac agaccgaccg
     9541 gaaagaacac caccgaagag agcgtcggca aagagatccc ttccaggcca aagtattttt
     9601 gtaccagtgg cgcagaccag tcggcgagtt taagtactac caccgcagca atcgccgctg
     9661 ccagcaaacc aaagaacagg ctgccggtgc tgtaataaac cagcgtaccg gcaagggcaa
     9721 aatgccagta gttccacata tctacgtcaa cggtgcgggt tttatttaac gccagcatcg
     9781 ccacgttaat cagaatagtc aggggaataa caaacggggc aattggcgaa ccccatgtaa
     9841 tagcggaaag cggcggccag cctaaatcca ccaccggtaa attaattccg gtgcgttcaa
     9901 ccatcgcctg ggccgctggc ccgacattgc tggtaagaat atcgaaaatg gcataaatac
     9961 cgacaaaacc aatgccgacc gttaatgacg aacgtaaggc tttagcgggt tttactttaa
    10021 aaaagagagc aagaataaat aacaccaacg gcaacattac cgtgggacca aatcccaata
    10081 tatattgcac aatattatag agggtatgcg cgatatcgtt cataataaac ctctttttca
    10141 ggcgagagcg cacctcgcgt tagcgcctga tggagtgggt tgaaattaat tattattaat
    10201 tactttttaa ggatcgaaac aatttgcgcc agtactttct cttcgccaat accggtaata
    10261 atgggtaagc cgttaaccac cggcacgtcg agttcataag gcactttagt cgttgaaaca
    10321 accagcgcta cgccgtcggt attaccgggt aaagaggcaa cgttggtttg ggaataattg
    10381 acgttaaggc cgtgctcttt gcaatactcc atgacttttt ctgttaccgc cgtggatgtg
    10441 gcaatacccg tggcgcagac aaataaaatg gtttgactca tatttcacct cgttctttca
    10501 ttttgtttaa gattactgtg tgtatttctg ttgtttccat cgtaagcagg gcgcgcataa
    10561 agggcgtatc ctgaatcatc tccataatcc agcccagggc atttaattgt ttattacttt
    10621 cgcttaaggc gagcaaaaaa atcaccctga caggtacggg gtcaggatcg ccgctcatat
    10681 cggcaaactc taccggttcc ggcaggatgc cgacggcaat tgcattgtgc ctgacatgtt
    10741 tatggtccgt gtgcggaatg gcgacgcacg gcggttcggt aggcagcccc gttgggtacg
    10801 tcgcttcacg tttcagtacg tgatcggcaa aatcagggac caccagctca gcagcaacca
    10861 acggttgcgc taactgacgg atgatctgtt caacgcttaa ccctgccgga agtcgttgaa
    10921 tataattttc ctgaaactgt atgccttgca ttttattcct cttaaaaaag tgtatttaac
    10981 tcaattcccg ccgttgcagc acggtgccca gataatattt atttcctttt aaccagacgt
    11041 tgccaaactc aacgggtaac tctcgggaga aaaagactaa ttgctccaga tgcaaaacgg
    11101 gtgcatcttc actgatatct aaaaaatgac cgcgttcatt accaattaat cgcgcggcat
    11161 agcgactttc cgagtaacga atttttcttt tcgacaaact ttctattgtt ggaaataaat
    11221 tgtgttgatt aaaatcgatt tccacgatgc cggggcatag ctcaatattg atacggttct
    11281 ctatcagcat cgctttttca tcaccaattg aacgtaaacg ctcaaggtaa agaatatcct
    11341 gaccgggcgt tattcttaat ttctctgcca cataacgatt agccggttca atccgtgacg
    11401 taataacttc agtggtaaag tgtattttct ggctttccag cgattccgcg aatgacaata
    11461 atccttcacc taacggatat gccacgttct cttttttgac aaatgttccc ttcccctgga
    11521 cctgtatcaa cacgccttct tttaccagtt gggcgaccgc ttttttaacg gttccccggc
    11581 tcacttccag catatccata atctggtatt ccgaaggaat gcgatcgtct tccaccagat
    11641 ccccggtata aatactttct cttatccact ccaccacttg ttgatagagc ggtatttgtg
    11701 acgacttact taatgatttc atggcgcgca cctgaacatt tacagttgtc tatggacaat
    11761 catggacaac ttcaagcgta tagtaggtga acgggagaaa aagaggatcg aagcgtctca
    11821 cattttgagg gaccaaatcg tgatcaaaaa aatgtgacgt gagtcgatat ttgtcagaag
    11881 tttactgcgc cgtcaggaaa taacggcgcg ttggtaggtg ttaatcttcc ttcggcaagc
    11941 actgcaaatg cccgtgccgc acgccgcgct gggcgatgac gtcatcggca aaatgctgca
    12001 cgtcgcccat gtcacctttc aacacggcga tttccagaca gtcgtcgtga ttaatgtgca
    12061 catgcagagt agcaactgag agatcgtggt gatgatgctg ggtggagaca atacggctgg
    12121 ctaagtcgcg tttttcgtgt tcatacacat acgacagcac cgcgaaacct tgtgtgccgt
    12181 gttgttgagt ggcttcctgc gccagagcgc tacgcagaat atcgcggatc gcctcggaac
    12241 ggttgttata accacgacgc tggctcaggc tgtccagcgt ctccagtaaa tcgtcatcaa
    12301 gcgtgatggt gactcgttgc atttgcgtta aaccttttct gtggtgcgac ggcgcacggg
    12361 gaatgcgggt aataccgcgt tttgtagcac acgtccggcg tcagaggaaa aggttaattt
    12421 atctcccacc acctgggttt cgacgatttg tccgttgtcc atcaccatca cccgctgaca
    12481 gaagcgttcc accaggcgta agtcgtgggt gatgaacagg caggcggtgc caaactgttg
    12541 ttgcagcttt ttcagcaggc gaatgacgcc cgcctgtaac acgagatcaa ggttcgaaac
    12601 cgcttcatcc agaatcagca gtttcggttc gaccgccagc gcgcgtgcca ggcagacacg
    12661 ctggagctgg ccgccgctta actgcggtgg gcgtttgtcg agcacgctgt cgtcgagatc
    12721 gaccgccttc agcatttcac tggcgcgcgc cagttgttcg gcttttttca gcgatagcag
    12781 gtggcgcatc ggttcacgca ggatctcgcg cacggttttg cgcggattca cggcgctgat
    12841 ggagtcctga aacaccattt gaatatcacg gcggaacgct ttacgttgcg cgcgattgag
    12901 cttcgccaac ggttcgccgc gccagctaat atttccctgt gagggcgact ctaaccctac
    12961 cagcagccgc gcgagggtac tttttccgca gccgctgcgt cccagcaggg cgacggtttc
    13021 accgcttttc agggccaggg aaacgttatt cagcaccgcc tggtgctgat gtttgccgct
    13081 aaatccaccg tgcgcatagt gatgggaaag gtcggagacg ttaagtaaag tcatgatgcc
    13141 agctccatac cgtagagggc gagatgagcg gaaaccaggc tgcgcgttac cgcatgtttg
    13201 ggggcgttaa acagcgtttc tacatcgccc tgttcaacaa tcttaccgtg agacattacc
    13261 gccacatcgt ctgccagacg cgctaccacg cccatatcat gggtaaccag cagcattccc
    13321 ggtgcttgtt tttgcataat gctttccagt agatcgagga tgcgtgcttg tgctactacg
    13381 tcgaggtcgg tggttggttc atcggcgatg ataaacggtg attcgcacag cactgccatc
    13441 gcaatcatca tgcgctgcaa catgccgccg ctcatctcga acgggtagag cttcagcacg
    13501 cgcgcggcgt tttccagccc caccgcttct atggcagcgg taagcgtagc gtcgtcggcg
    13561 ggtttcccca gcgccaggca ggtttcacgc gcgtgagtgt gcatggtgtg cagcggatta
    13621 aaggcgctgc gcgggttctg catgatggtg gcaattttga tgccgcgcag ggcgcaggga
    13681 gaaaccggtt ttccatcggc caaaatttcc cccgccgtct ggcgaacgcc agcaggcaga
    13741 atgcccagcg tcgcggcgca ggtcagcgac ttcccgctgc cactaccgcc gactaacgcc
    13801 agcacacgcc cacgttgcag ggttaacgat acgccgtgca ccagcggctg cgcggcctgt
    13861 agcgcgatat tacggagttc aatctgttgt ggcattagtg tgcgtgctcc gtcaccagat
    13921 gagggtccag atgatcgcgc agtgcgtcac ccaccaggtt aaaggccatc acgctgataa
    13981 acagcgccag ccccggccag aacatttgca gcggctgggt ccagatatac tgacgcgcgt
    14041 cgttaatcat cacgccccat tcggcggtcg gcgcggtcac gccgagaccg aggaaggaca
    14101 tccccgcgac gtgtagcatc atatgaccga tatccagcgt cgccagcacc agcagcgagg
    14161 ggatcaccgc acctgccaga tgatcaacaa acacccgcac atggcccgcg ccagaaagcc
    14221 gtgatgccag cacaaactcg cgctggcgca gtgaaatcac cagactgcgc accatgcgcg
    14281 cgtaccatgc ccagtgcgaa agggcgatag cgataattac gttggtcaac ccggtgccaa
    14341 gcacgccgac cataaagaac gacagaatcg aggttgggaa ggtcataaac atatccgcga
    14401 cgcgcatggt ggcctgatca acgcgcccgc caatcagccc ggcgctgccg ccaataatca
    14461 gccctaatgt cagcaccaac agcaggcagg ccattaccga gccgagcgac acgcgggtcg
    14521 ctgccatcag ccgcgagaaa atatcgcgcc ctaaatgatc ggtgcccagc cagtgctgcg
    14581 catccggcga gagcaggcgc gacggcaaat caatagcctg agggtcatac ggcagccacc
    14641 actggctggt gagcgcaatc agcgccagca gggcgataat gatcagcgcc aggcgaaccg
    14701 accagcggga ggagaggaaa aagttcacgc gtgcgctcct tcatgacgac gaatacgcgg
    14761 gtccagcgcg gcgttgagca aatcgacaat caaattacag accacaaaaa ccaccaccat
    14821 catcagcgta aagcactgga tcaccggata gtcacggtta aaaatcgccg acaccgcata
    14881 gcgcccgacg cccggccagg caaagatgtt ttcgataatc atcgtcccgc caatcagttc
    14941 gccaatgtgc atccccacgg cagtgatcat cggcagcgag gcattgcgca gaatatggcg
    15001 gcgttcggtc tgtttgtcgc tcagaccacg cagtcgtgcc caggtgacgt gacgctgacc
    15061 ggcgacgtcc agcatactgg cgcgcagcaa acgcgcgtta atcgccagcg acataaaggt
    15121 gatggatacc gcaggcaaaa tgatgtgctg ccagccgccg taacccattg cgggtagcca
    15181 ttgcagatac accgaaaacg ccatcaccag taaaaacgcc agccagaagt taggcatcga
    15241 cacgccgaga aacgcgatga aacggacggc gaaatccggc agacgatcgc gatggcgcgc
    15301 cgcccagata ccgagcggta cggaggtgag cagaattaat accagcgccg cacctgcaag
    15361 ttccagcgtg gcgggcagga aattcagcat atcgtccagc accgggcgtt ggctggcgaa
    15421 tgagatacca aagtcaagat gcagcgcctt ccacaaccag gtgccgtact ggacatacag
    15481 cggctgatcc agccccagca tggtgcgggt agaggccagc atctccggcg tcggcggcag
    15541 gttagataaa cgcaaataat caagcgccgg gtcgccggtg ccgaggcgca gcatcaaaaa
    15601 aatgatcacc gaggcggcga gcaccatcgg gatcagcagc agaaagcggc gtaatacata
    15661 acgcaacatt aaggtttcac cggtttaatc tgttcgaacg ggatttcggt ggcgatcggc
    15721 gcgtagggga tgttacccag ttccggtttt gaaaccacca tcattgagat gtaactgata
    15781 ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt cgcgatacag cgcctgacgt
    15841 tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt tatcaatcag cggcttgtcg
    15901 gctaaacctt gctgtgcctg gaagtcagcg tgcgacggta cgcgcattga actcaggaag
    15961 gcgtgtggat cgtatggcgc gccccaggtg cggtggaaaa tcatgccaaa acgaccgtcg
    16021 cgctgacgag catagatact gctctcttct tcgccaatca gcgagacatc tgcgccaatc
    16081 tggcgcatat cagcctgaat gatttccgcc atcgatttgc ttaacgcatc ggtgccgatg
    16141 aacgacagtt caatgcgcag cggctgacca tttttctcgc ggatgtcttt gcctgcaggc
    16201 agcgtccaac cggctttttc cagcaacgct ttcgctttct gcggatcgta ctggcgcggt
    16261 ttcaggccga ggttggcgta gggcacagaa ggggcaaaca gtgtgtcggc gacctgctgg
    16321 gtaccataca acgcgttatc aatcagcgat tttttgttca ccgcgtaatt aagagcttca
    16381 cgtactgcca gctcgttggt gggggctttg gcggtattga gcgccagcat cacggtttcg
    16441 atcggctgtg acagttgagt gtggtaagcc gggttctggc taaagcgggc gaaggtatcg
    16501 agcggtaata acccttcgtt tccgtacagc aggtcgatat cgccagtttc aaacgccacc
    16561 gcgcgggtag tcgggtccgg gatcacgtta aaggtgatct ttttaatcgc cggcttttcg
    16621 ccccagtagt tttcgttacg gacgaagaca tcgtactgat tcagtttcga ttcctgtaaa
    16681 acccacggcc cggtgccaat cggcgcttta attccgttca tggtttcgtg gtttttaaac
    16741 tgcgagggtg cgataaaacg gaaaggacgg ggcagggcca gttcttgcag gaaaggatag
    16801 taggcgcttt tcagggtgat ttgcagctcg tttttactga gtgctttgac atcaacaatc
    16861 tggtttgcca gctccagcca ggcgtgacgt tgacggttat cgagcactgc acggaagttt
    16921 tctgccgccg cctctgcatc aaacggttcg ccgttggaga atttcacgtc atcacgcagg
    16981 gtgaaggtcc aggttttacc atcttctgaa tgagtccagc tttttgccag ccacgggatc
    17041 accgaaccgt ctgcctgata tttcaccagc ggttcataaa ccatgctctg ggcgaacatt
    17101 tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat tcaccggcca ggcggtggtg
    17161 atttcatctg gtgcagcggc atggacgata aaagacgcac aagccagcag cgcaaataga
    17221 gtgcggcgga gtgtagagaa catggtaacc ccaatggatt aaaatagatg gcggaaataa
    17281 gtatgacgat tttaagtatt cgtcatactg attacctgtt ctggatcaat agatgggcag
    17341 tcgggtgggc ggatcagtta actgaatcga tccattgcac actgtcggcg gtgagcgtaa
    17401 agggggtagg ggtgcagatc gccaggctta aattttctag ctgacaatgg ctgactgaca
    17461 gcgaggagtt ataggtgctg tctacgctga cgatttgcca ggcgctgccg ccacgctgtt
    17521 taacgatggc ttctttgcgc gtccagatgc gccagaacat ttccagttgc tgatcaggat
    17581 gcacggcgtc catctcggca tgttccccaa ggctgaatac ggcgttcgcc agccagcgcc
    17641 agttggcgcg cgggcgaatc acttcgatat cgcagccgac ttcgccttca tcgctcaaca
    17701 gcagggcgat atcgtcaccg ctatggctta agttgaacca tagcggcgtt tccggcgcga
    17761 atgcaggttt gccttgttcg ccatagatga tctccggtag cggggaaagc gtgtgcgaaa
    17821 gcaatgcacg ccccgccagc cagcgttcac gtcgtggacc ttgcggtgct tgctcgcgta
    17881 aacccggtgg cagaggagct gcgcttaagg tcgaaacttt ccccagaact atccgataca
    17941 tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc gtaacacttg tcgcctcatc
    18001 ccggtaaccg acttttcggt ctgcctggcc ccagtaaaat cgccagtttg ctgccgcctt
    18061 tggtggtttc catccagatt ttacacacgc tggttaatgg caccgaaagt agcatcccta
    18121 ccgggccgag cagccatccc caaattaaca atgaaagaaa taccaccatg gtggacatcc
    18181 ccaggcgatg gcccatcatc cgtggttcta aaatattgcc gatgaccata tggaccacta
    18241 aaaacaatgc gccgaccaga atacattcgt aaataccatt aaacagcagc acctgaatca
    18301 ttggcggtac ggcggaaatt accgcaccga tattgggcac gtagttgagc aaaaacgcca
    18361 gtactgccca catcagcgca aactgcaccc ccatcagctc cagccccagc cagacgatta
    18421 tgcctgtcca taaactgagt agcgtcttta acgccagata atgcgaaacg cctttaagtg
    18481 cgcggtgtag tccggcgatg tgaatctgtg gattattcaa cgcgaaacgc attttgtaag
    18541 ggacgtggcg cacttcaaac agcataaaaa ctacggtcat caccagcaaa agcacgctcg
    18601 ccattgcccc ggaaagcccg gtcattagcg ccgtggtgaa ggtaaccact ttttccgagt
    18661 ccatccgctg tagtattcgc tccggcgaca tatgcaaatt aagaaaaggc aacatctcct
    18721 gcaatttaaa aagtttgcgc gtcagctcct tattaaactt cggcagcata gagataaatt
    18781 cgttaaacga tgccgccagt acgccgacca gcgcggtgag tgcgatcagc atcaccacaa
    18841 ccacaatcgt aatggcaacg gggcgttgta ctccccgacg aataaaccag gtgacgagcg
    18901 ggttgagaac gatggcaaaa aacagcgcca gtaatagctg cacaatgata tctgccgctg
    18961 cgtgaatgcc cgcgaggatc actaccagcg aggccaactt gagcagaatg cgcatgcccg
    19021 ttttatcggg ttgaggggtt tccattgggg cttccttgtg actttttgta ttaagtgtag
    19081 cgggagtcac gccagcaata ttctgattct tactgctgat tttcacgtca gcacatggta
    19141 aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc ccgaacccgt agccgaaccc
    19201 gcgctaaacg gattgcgcct gaatttgcgc attgtctcca ttgtcatgtt taacttcgcc
    19261 agctacctca ccatcgggtt gccgctcgct gtattaccgg gctatgtcca tgatgtgatg
    19321 ggctttagcg ctttctgggc aggattggtt atcagcctgc aatatttcgc caccttgctg
    19381 agccgtcctc atgccggacg ttacgccgat ttgctgggac ccaaaaagat tgtcgtcttc
    19441 ggtttatgcg gctgctttat gagcggtctg ggatatctga cggcaggatt aaccgccagt
    19501 ctgcccgtca tcagcctgtt attactgtgc ctggggcgcg taatccttgg gattggacaa
    19561 agttttgccg gaacgggatc gaccctgtgg ggtgttggcg tggttggttc gctacatatc
    19621 gggcgggtga tttcgtggaa cggtattgtc acttacgggg cgatggcgat gggggcgccg
    19681 ttaggtgtcg tgttttatca ctggggcgga ttgcaggcgt tagcgttaat cattatgggc
    19741 gtggcgctgg tggccatttt gttggcgatc ccgcgtccga cggtaaaagc cagtaaaggc
    19801 aaaccgctgc cgtttcgtgc ggtgcttggg cgcgtctggc tgtacggtat ggcgctggca
    19861 ctggcttccg ccggatttgg cgttatcgcc acctttatca cgctgtttta tgacgctaaa
    19921 ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct gtgcgtttgt cggtacgcgt
    19981 ttgttattcc ctaacggcat taaccgtatc ggcggcttaa acgtggcgat gatttgcttt
    20041 agcgttgaga taatcggcct gctactggtt ggcgtggcga ctatgccgtg gatggcgaaa
    20101 atcggcgtct tactggcggg ggcggggttt tcgctggtgt tccctgcatt gggcgtagtg
    20161 gcggtaaaag cggttccgca gcaaaatcag ggggcggcgc tggcaactta caccgtattt
    20221 atggatttat cgcttggcgt gacgggacca ctggctgggc tggtgatgag ttgggcgggc
    20281 gttccggtga tttatctggc ggcggcggga ctggtcgcaa tcgcgttatt actgacgtgg
    20341 cgattaaaaa aacggccccc ggtggaaata cctgaggccg cctcatcatc ttaaaactta
    20401 ctgaataacc agcgtattaa tgatgttttc tgcggtggtc tgtgcttttt gctgattgtc
    20461 agcgggcagc gtaatttgca tggttagcag ttgattaccc acgttaccca gaataacgga
    20521 agagtacgcc gtctggcctt tcgcggagat aatgctgtct aactgctgca ttttgtgacc
    20581 tttcagctca atggctttat tggttaccac ttgcagctgc ggatcgcggc tacgttgctg
    20641 atcttccaga cgcttcgcca gcaccgccag atcttctttc ggatcatcgc ccatgatgac
    20701 gatgactgct ttctgcccgg tggcgtcgga ccagacatgc atgttattgg cctgcgttcc
    20761 cagcttaccg ctctggtcgg tcatatccgc tggcagcgag aaacttaact tgccatcaag
    20821 caggttgacg ggattcccgg tcgcgttact ttctgcgacc gaaccctgcg ccgtagcgtt
    20881 agtgtcttta tcatcacagg ccgcaagccc cataaccagc aggccaattc cgacatattt
    20941 aaccagattg cgcattgact tcttcctttc gataaacggc cataacggct cattcatcca
    21001 tcttatcaca actctgataa cgaaccttta actcgcctgc aaagcgttga tttcggattt
    21061 atctgccagt cttttcaaca gcatattgag taatacgcca tacattggca ggaagaaaac
    21121 gatactgatt aacactttga aacagtaatc gaccagcgcg atttccatcc agtgttcagc
    21181 cataaaggca tccgggctac gccagaaggc aatgaagaag aaggccagcg tgtcgctgac
    21241 gttaccaaac agggtggaag ccgtcggtgc cagccaccag cgacgactct gacgcaggcg
    21301 gttaaaaacg tgcacgtcga ggatttgccc cagcgcgtag gccatgaaac tggcggtggc
    21361 gatacgggcg acaaacaggt tgaagtgggc gagcgcgtcg aatccctgcc aggaacccat
    21421 atagaacagc gacgagatga cgtaggagat cagtaacgcc gggatcatca ccgcaaagat
    21481 gatgcgacga gccagcggtg cgccaaaaat acgcacggtc aggtcagtag cgagaaagat
    21541 aaacggaaag ctaaacgcgc cccaggtggt atgaaaacca aaaatggaga ccggaagctg
    21601 aaccagatag ttactggagg tgatcaccag cagatgaaat aacgataacc agaacaacgc
    21661 cttatagcgt tgcgtttgcg agaaaacgtt catattgtac ctttttgatt aaccattggg
    21721 gtgagggaac ccaatacgta cgacacgtct gctaccttaa cataataacg tgctgaccct
    21781 tttttcgagc cgccgcatga tactgcttta cgttgacaat gcaatggtta attttcacgc
    21841 aatcgttaac ctggtttgct tacggactca cagggcgtaa actagcgccg tttttttatg
    21901 tgatgagaag aaaatgaccg atctcttttc cagtcctgac catacactcg acgcgcttgg
    21961 cttgcgctgc ccggaaccgg tgatgatggt gcgtaaaacg gtgcgcaata tgcaaccagg
    22021 cgaaacgttg ctgattattg ctgacgatcc ggcgacgacc cgcgatattc ctgggttttg
    22081 tacctttatg gaacacgaac tggttgctaa agagacggat ggactgcctt atcgttattt
    22141 gattcgtaaa ggcggttgat gggggaagat gggcttcgat actgcctttc ccttaccctc
    22201 tccccagaga ggcgagggga ccgatcgcac tcaatgttgc tgcttgaaac cggtttgtag
    22261 gccggataag gcgttcaagc tgcatctggc gatacggtgc acaatgcctg atgcgacgct
    22321 gtcgcgtctt atcatgccta cgagttcgat gctctgtata ggccggataa ggtgttcacg
    22381 ccgcatccgg tattgttgga ccgtagactg atagcctctt tatttcctgc gcaataaccg
    22441 taacgcattc gccgttacca gcaccgtcgc accggtatct gccagcactg ccagccacag
    22501 gccggtcatc cctaacagcg tggtgacgag gaaaacgcct ttcagcccca gcgcaatggt
    22561 gatgttctgg cggatattgg cgtgagtggc gcgtgccagt tcaatcattt gcaccaggcc
    22621 gcgcaggtgg ttatgggtta atgctgcgtc ggcggtttcc agggccacgt ctgtgccact
    22681 gcccattgca atcccaatag cggcggcttt catcgctggc gcgtcgttaa taccgtcgcc
    22741 gaccatcgcc agcggcgcat gttgattcag ctcggtcacc gctttaactt tatcttccgg
    22801 caagaggccc gctttaaatt ccagccccag ttccccggcg attgccgctg ctgcgcgtgg
    22861 attatcgcct gtaaggatca cccctttgac gcccagcgcg ttcagttcac tgatagcagt
    22921 tgcagcatcg gcgcgcaggg tatcctgcaa cgcaatgaca cccagcacgt catcattacg
    22981 tactaccagc actaccgttt gcccggcgct ttccagttca ttaatcaaac cagcaaatgc
    23041 atcagcagga tgttttcctg cagcgcatat caacacgcgc tcaccgttaa cctgcgcttc
    23101 aatgccagac ccgaccagcg cccgctgtga ttgggcggtg ggaatggcga gttcagcaac
    23161 ctgtgcttcg cgcacaatgg cctgtgccag tggatgcgtc gcgccttgct cgaccgccgc
    23221 cgccagtgtc agcagttcag attcactaat acccgttgcc ggatgaatcg cggtaacgcg
    23281 cggtttaccg acggtcagcg taccggtttt atcaaacgcc acctgggtaa cacgacccag
    23341 ctgctccagc gccgcgccgc ctttaatcaa cgccccacga cgcgctgctg ccgccaaccc
    23401 ggaggtaatc gccgcgggcg tggagataac taaagcacat gggcaaccaa tcagcagcag
    23461 cgtcagccct ttataaatcc actcctgcca gctggcggca aacagcagcg gcggcaccag
    23521 cgttaccagc agggcgacgg ccataatcgc tggcgtgtaa atacggctga aacggtcgat
    23581 aaaccgctca ataggtgcgc ggcgctcttc ggcttcttca atcagtttca gaatccggtc
    23641 aatggcgctg gcacccggtt ctgacagcac ttccagcgtt accagacggt ctacgctggt
    23701 ggtgcctgca ggaactttat cgcccgtcgc gcgctccaca ggaatggatt ctccggtcag
    23761 ggcactttca tcaaaactgg caaacggtga gagcagttta ccgtcggcag gcaaacgccc
    23821 acctgccgca acttcaatca catcgccagg ccgcaggctg tttattgcca cctcttcacg
    23881 ctcaccgtta cgcagacgcg tggcggtttc tggcttcagc gccattaacg cgctgacccc
    23941 ttgacgcgcg cgactggcgg cccagccttc cagtcgttca ccaatcaaaa acagcagcaa
    24001 caccatcgca gcttcagccg ttgcgccaat aaacagcgca ccaatagcgg ctacgctcat
    24061 taaggtttca atggcgaagt agctgccaga tttgatcagc cgtaatgcct gacgagcaat
    24121 tgggtacagc ccaaccagcg tggtcgcgat aaacgccagt tgaccaaacg gatgattgaa
    24181 ctgctccagc ccccaactga ttgccatcat cacgataagc gtaatcagcg gcagattctc
    24241 tttcaggcgt gattcttgtg gttcgtcgga ggcctgttca tcgcgcaggg aatagcccgc
    24301 tttttgcacc gcagattcaa cttgtgcccg gatgtcattg tcggcatcca ccaccagttt
    24361 ttcggtggcg aacaacacct gtacctgatt cacgcctgca agctggcgca cggcattttc
    24421 cactttgcgc gcacaggcgg cgcagtccat accgctgact ttccagctat agcgggtgcc
    24481 ggagacgttt tcagagagag ttggcgtgct ggaacatgcg ccgtcgcagc aacagtcgtt
    24541 ggtgttctgt accgtagtta gcggtttgaa cgcagcaaat tgaggggctt tcttgccgtg
    24601 attgtcagga gtcgacatgg catcctccgg ttaagttttt tctcattaac cgaaggatac
    24661 actctggagt cgactccaga gtcaagtttt atcagagata cagcgagcgg acgatgagga
    24721 agtgaccagc aaagtagcag gccgcggcaa tggcgttatc cgcacggaag cgtcggcgat
    24781 agtgactacc cagccagaca aagttactga taaacagcaa cgaagcgccg acaaacgcag
    24841 agagcgccgg agcggtcgga cggaagaacc acagttcacc tgccagccac accatcacca
    24901 gcgtcatgcc gataaacgtg cagataggcc aacgatactc ttccagacgc gtccagataa
    24961 tcgccagtaa cagcgcgccc agaaccagca gcaccaacgg cagaggccag aagaaagaga
    25021 gcgtcatctg actggcgaaa tagatggtgt acagcaggtg cgagaggaaa aacgcgccga
    25081 tggcgtacat cagacgttga cgtggcaaca gggttagcgc atcgcccagc agtgaggcac
    25141 acagccccgc cagcaccaga tagctaatgg cgtcgaacat cggcgcttgc caggccagca
    25201 acagcaggag aagaagggtt aacggtttaa acacccagcg ttgccaggtt ggcccacgat
    25261 acgatgcatc cacagatagc catgcggaaa gacagacagc gataaacgac caaagcatct
    25321 tagttccttg aattcgttat tttttcgtaa gcggaatgcc tacggtcttg tttccagttt
    25381 agtggcgagg gtggacggat gacaacacca taactggcag ggtatgctta tttccgcatt
    25441 ttccgatgag ggataaagat gagtaaacca cctcttttct ttattgttat cattggctta
    25501 atcatcgtcg ccgcatcgtt tcgttttatg caacaacgac gggaaaaagc tgataatgat
    25561 atggcaccgc tccagcaaaa gctggtggtg gtgagcaaca agcgggaaaa accgattaac
    25621 gatcgccgtt cgcgccagca ggaagtgacg ccggcaggca ccagtatgcg ctatgaggca
    25681 agcttcaaac cgcaaagcgg aggaatggag cagacgtttc gcctcgacgc ccagcagtac
    25741 cacgccctga cagtgggtga taaaggtacg ctgagctata aaggaacgcg ctttgtcagc
    25801 tttgtaggcg aacaataagg ttatttttta actttaaatt tcttctgcca gaccagcagt
    25861 tcaaacacgc cgaaaaggaa aatccgcacc ttttcggcgg tggtcatctg cgggccatct
    25921 ttcggtaaag tggatttcaa tagcgccagc tgcataccat gcatcagcac ggtaaaaatc
    25981 agcgccacgt taacgaaaat attcagcggg cgtgggaagg gatgcaccag gttgaggatt
    26041 aaaaatcccc aaacacagag cattaacaaa cgaccaatat taatcagcat cactttctcc
    26101 ttgtgcttcg cgttgataca gccgataagc cacctgaccc gccacttttt cccgatgtaa
    26161 tgaccagttt gccggaacag tgggcagacc gttttcgact tcgctttcga cataaatcag
    26221 ggcttcgtca gccagccagc cgttatcttc cagtaaattt atcgtctctt ctaacaagcc
    26281 acggcggaac ggtggatcga caaacacgat attatgcggt gtgccttttt gcgccaggaa
    26341 tgacatcgcg ttgctgttca ccacgcgtgc attgcctgct tttagtgtcg ccagattctt
    26401 aattaactgc tgagaaaccg cacgatccat ctcaatcaac gtggcccccg cagcgtagcg
    26461 cgataacgct tccagcccca gcgcgccgct tccggcgaag caatccagac attgggcgtc
    26521 aacaatgacc ggagccagcc agttaaacaa cgtttcgcgt acgcggtcgg tggtggggcg
    26581 cagacctggg ctatcaggaa ccgggagttt acggcctcgc cactgcccgc caataatgcg
    26641 gatttggccg ctgccggaat gattcggttt tttcatgata aattgctcaa tccgccagat
    26701 aacacatact tgcaggcggt gatgtgaatt aagttaagtg atagactatt tcatcatttt
    26761 tttagctgct atgtacatag cgttaacgct gtgccatgaa gcaacagcga ggagtgtagt
    26821 cgcaaatggc gaaagaaaaa aaacgtggct ttttttcctg gctgggcttt ggtcaaaaag
    26881 agcagacccc ggaaaaagag acagaagtcc agaacgaaca accagttgta gaagaaatcg
    26941 ttccggcgca agagcctgcg aaggcttctg aacaaaccgt tgaagagcag ccgcaggcgc
    27001 atactgaggc cgaagcggaa acttttgctg ccgacgttgt ggaagttact gaacaggttg
    27061 ctgaaagtga aaaagcgcag cctgaagcgg aagtcgttgc acagccggaa ccggtcgtag
    27121 aagaaacgcc ggagccagtg gctatcgaac gtgaagaact gccgttgccg gaagacgtca
    27181 acgccgaagc ggtttcgcca gaagagtggc aggccgaagc ggtttcgcca gaagagtggc
    27241 aggccgaagc ggaaaccgta gagattgtcg aagcggcgga agaagaagcg gctaaagaag
    27301 aaatcaccga cgaagagccg gaggcacagg cgctggctgc cgaagtggca gaagaggcgg
    27361 taatggtggt ttctccggca gaagaagagc agccggtgga agaaatcgct caggagcagg
    27421 aaaaaccgac caaagaaggt ttcttcgcgc gcctgaaacg cagcctgtta aaaaccaaag
    27481 aaaatctcgg ttccggattt atcagcctgt tccgcggtaa aaaaatcgac gatgatctgt
    27541 ttgaagagct ggaagaacag ctgttgatcg ccgatgtggg cgtggaaacc acacgtaaaa
    27601 ttatcaccaa tctgacggaa ggcgcatccc gcaagcagct tcgtgatgcc gaggcgctct
    27661 atggcctgct gaaagaagag atgggcgaga ttctggcgaa agtcgatgag ccgctgaatg
    27721 ttgaaggtaa aacgccgttc gtgatcctga tggtgggcgt caacggtgtg ggtaaaacca
    27781 cgacgattgg taagctggcg cgtcagtttg agcagcaggg taaatcggtg atgctggcgg
    27841 caggcgatac tttccgtgcg gcagcggttg aacagcttca ggtctggggt cagcgcaaca
    27901 atattccggt gattgcccag cataccggtg cggattccgc ttctgttatc ttcgacgcca
    27961 ttcaggccgc taaagcgcgt aatatcgacg tcctgattgc cgataccgcc ggacgcctgc
    28021 agaacaaatc gcacctgatg gaagagttga agaaaatcgt ccgcgtgatg aagaaactcg
    28081 acgttgaagc gccgcatgaa gttatgctga ctattgatgc cagcaccggg cagaatgcgg
    28141 taagccaggc caaactgttc cacgaagccg ttggcttaac cggcatcacg ctaacgaaac
    28201 tggacggcac ggcgaaaggc ggggtaattt tctcggtggc tgaccagttt ggtatcccta
    28261 tccgctacat tggtgtcggc gaacgtattg aggatttgcg tccgtttaag gcggacgact
    28321 ttatagaggc actttttgcc cgagaggatt aacaatgatt cgctttgaac atgtcagcaa
    28381 ggcttatctc ggtgggagac aggcgctgca gggcgttacg ttccatatgc agccgggtga
    28441 gatggcgttt ctgaccggtc attccggcgc agggaaaagt accctcctga agctgatctg
    28501 tgggattgag cggcccagcg ccgggaaaat ctggtttagc ggccatgaca tcacgcgtct
    28561 gaaaaaccgt gaagttccgt ttctgcgccg ccagattggc atgattttcc aggatcacca
    28621 tctactgatg gaccgtactg tctatgataa cgtggcgatc ccgctgatta tcgccggtgc
    28681 cagcggtgat gacattcgtc gccgggtgtc ggcggcgctg gataaagtcg ggctactgga
    28741 caaagcgaag aacttcccta ttcagctttc cggcggtgaa caacagcgtg ttggcattgc
    28801 ccgcgcggtg gtgaacaagc ccgcggtact gctggcggac gaaccgactg gtaacctgga
    28861 cgacgcgctg tcggaaggca ttttacgtct gtttgaagag tttaaccgcg ttggagtaac
    28921 cgtattgatg gcaacgcacg acatcaacct gatttcgcga cgttcctatc gcatgctcac
    28981 cctgagcgat ggtcacttgc atggaggcgt gggccatgaa taagcgcgat gcaatcaatc
    29041 acattcggca gtttggcggg cgtctggatc gcttccgtaa atcggtcggc ggctctggcg
    29101 acggcggtcg taacgcgccg aaacgcgcga aatcctcgcc aaaaccggta aatcgcaaaa
    29161 ccaacgtttt caacgaacag gtacgctatg ccttccacgg cgcattgcag gatctgaaaa
    29221 gcaaaccgtt cgccacgttt ttaacggtga tggttatcgc catctccctg acgctgccca
    29281 gcgtctgtta tatggtgtac aaaaacgtta accaggcggc gacgcaatat tatccgtcac
    29341 cgcaaatcac tgtttatctg caaaaaacgc tggatgatga cgccgctgcg ggcgtggtgg
    29401 cacagttgca ggccgagcaa ggcgtggaga aagtgaatta tctttctcgt gaagacgcac
    29461 tgggcgagtt ccgtaactgg tctggttttg gtggtgcgct ggatatgctg gaagaaaacc
    29521 cgcttccggc agtggcggtg gtgatcccga aactcgattt tcaggggacg gagtcactga
    29581 atacgctgcg tgatcgtatc acgcagatta acggcattga cgaagtgcgg atggatgaca
    29641 gctggtttgc ccgcctggcg gcgttgaccg ggctggtcgg gcgcgtttcg gcaatgatcg
    29701 gcgtattgat ggtggcggca gtgttcctcg tcatcggtaa tagtgtgcgc ctgagtatct
    29761 ttgctcgccg tgattctatt aacgtgcaga aactgattgg tgcgacggat ggtttcatcc
    29821 tgcgtccgtt cctgtacggt ggcgcattgc tgggattttc tggcgcattg ttgtcactaa
    29881 ttttgtcaga aattctggtg ctgcgtttgt catcggcggt tgcggaagtg gcacaggttt
    29941 tcggaacgaa gtttgatatc aatggcttat cgttcgatga atgcctgcta ttgctgctgg
    30001 tatgctcgat gattggctgg gtggcagcgt ggcttgccac ggtacaacat ttacgccact
    30061 ttacgcctga ataataaaag cgtgttatac tctttccctg caatgggttc cgtagcaggg
    30121 aaagagaccc cgttgtctct tcccggtatt ttatctctat gtcacatttt gtgcgtaatt
    30181 tattcacaag cttgcattga acttgtggat aaaatcacgg tctgataaaa cagtgaatga
    30241 taacctcgtt gctcttaagc tctggcacag ttgttgctac cactgaagcg ccagaagata
    30301 tcgattgaga ggatttgaat gactgacaaa atgcaaagtt tagctttagc cccagttggc
    30361 aacctggatt cctacatccg ggcagctaac gcgtggccga tgttgtcggc tgacgaggag
    30421 cgggcgctgg ctgaaaagct gcattaccat ggcgatctgg aagcagctaa aacgctgatc
    30481 ctgtctcacc tgcggtttgt tgttcatatt gctcgtaatt atgcgggcta tggcctgcca
    30541 caggcggatt tgattcagga aggtaacatc ggcctgatga aagcagtgcg ccgttttaac
    30601 ccggaagtgg gtgtgcgcct ggtctccttc gccgttcact ggatcaaagc agagatccac
    30661 gaatacgttc tgcgtaactg gcgtatcgtc aaagttgcga ccaccaaagc gcaacgcaaa
    30721 ctgttcttca acctgcgtaa aaccaagcag cgtctgggct ggtttaacca ggatgaagtc
    30781 gagatggtgg cccgtgaact gggcgtaacc agcaaagacg tacgtgagat ggaatcacgt
    30841 atggcggcac aggacatgac ctttgacctg tcttccgacg acgattccga cagccagccg
    30901 atggctccgg tgctctatct gcaggataaa tcatctaact ttgccgacgg tattgaagat
    30961 gataactggg aagagcaggc ggcaaaccgc ctgaccgatg cgatgcaagg tctggacgag
    31021 cgtagtcagg atatcatccg cgcgcgctgg ctggatgaag acaacaagtc cacgttgcag
    31081 gaactggctg accgttacgg tgtttccgct gagcgtgtgc gtcagctgga aaagaacgcg
    31141 atgaaaaaat tgcgcgctgc cattgaagcg taatttccgc tattaagcag agaaccctgg
    31201 atgagagtcc ggggtttttg ttttttgggc ctctacaata atcagtttcc ccctccggca
    31261 aaaggccaat ccccacgcag attgttaata aactgtcaaa atagctattc caatatcata
    31321 aaaatggggt atgttttagc agagtatgct gctaaagcac gggtagctac gtataaaacg
    31381 aaataaagtg ctgcacaaca acatcacaac acacgtaata accagaagag tggggattct
    31441 caggatgaac ataaagggta aagcgttact ggcaggatgt atcgcgctgg cattcagcaa
    31501 tatggctctg gcagaagata ttaaagtcgc cgtcgtaggc gcaatgtccg gtccggtggc
    31561 gcagtacggt gaccaggagt ttactggcgc agagcaggcg gttgcggata ttaacgctaa
    31621 aggcggtatc aaaggcaaca aactgcaaat cgtaaaatat gacgatgcct gtgacccgaa
    31681 acaggcggtt gcggtggcga acaaagtcgt taacgacggc attaaatacg tgattggtca
    31741 cctctgctct tcatcaacgc agccagcgtc tgacatctac gaagacgaag gcattttaat
    31801 gatcacccca gcggcaaccg caccagagct gaccgcccgt ggctatcagc tgatcctgcg
    31861 caccaccggc ctggactccg accaggggcc gacggcggcg aaatatattc ttgagaaagt
    31921 gaaaccgcaa cgcattgcta tcgttcatga caaacagcag tacggcgaag gtctggcgcg
    31981 tgcagtgcag gacggcctga agaaaggcaa tgctaacgtg gtgttctttg atggcatcac
    32041 cgccggggaa aaagatttct ctaccctggt ggcgcgtctg aagaaagaga atatcgactt
    32101 cgtttactac ggtggttatc acccggaaat ggggcaaatc ctgcgtcagg cacgcgcggc
    32161 agggttgaaa actcagttta tggggccgga aggtgtggct aacgtttcgc tgtctaacat
    32221 tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg aagaactacg atcaggttcc
    32281 ggcgaacaaa cccattgttg atgcgatcaa agcgaaaaaa caggacccaa gcggcgcgtt
    32341 cgtctggacc acctacgccg cgctgcaatc tttgcaagcg gggctgaatc agtctgacga
    32401 tccggcggag atcgctaaat acctgaaagc gaactcggtt gatacggtga tggggccgct
    32461 tagctgggat gagaaaggcg atctgaaagg ctttgagttc ggcgtatttg actggcacgc
    32521 caacggcacg gcgaccgatg cgaagtaatc attaatcggc aactttgggt tgccgccaaa
    32581 ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg taaacgcctt atccggccta
    32641 caagattcaa agaaatcagt aaattgcagc acacattgta ggcctgataa gcgtagcgca
    32701 tcaggcaata tcacttgtga aatcaaactt gacgattaac gcttctccca gccaccctgt
    32761 tgtgccgtaa atcccagtgc tcgcataaac gccgtcatca cgccgcgatc ttccacgcca
    32821 gcatccgcca tccaccaaca tgaaacgtca ggattgttac gcaaaacctc tttcagcaga
    32881 tattgcccca caccgcgacg tcgggtgact tcccgcacgc gtagagaatc cagtgccccc
    32941 tcggtgccgc ttaaggttac ccgcacggca gcgagcaggc gctcgttaaa acgcgcggcg
    33001 tagatacggt ggttatcgtc aacctgtaaa gaggaagggg aatactccgg ccagatcttt
    33061 tgcaggtcaa tccggtcctg gtcgctaaag ttttctaatc gaatgatggt cagcttcatg
    33121 gggaacccgt gtaaatcaca aaagtggaac cagtgtagcg aaataattta atcggaggct
    33181 ttctc
//
LOCUS       DADSZD010000033        32670 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.033, whole genome shotgun sequence.
ACCESSION   DADSZD010000033 DADSZD010000000
VERSION     DADSZD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.033"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            19..1152
                     /gene="hcp"
                     /locus_tag="J1Z28_001437"
     CDS             19..1152
                     /gene="hcp"
                     /locus_tag="J1Z28_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBA7716283.1"
                     /translation="MSDEIYLTITGEQQGCISSRCGTSASIGNRRQIGHEDEIFAFSL
                     SNSITNTGKGSQLHGLSFCKLIDKSSPLLINAINNNEQLFMEFDFYRINRFGRWEKYY
                     NIQLRGALLSAINHLFTENNLDTETITVSYEYILSKHLIANTEFSYLAFPDNYNRLFI
                     PRQKTKDDNGLKTLNSKAVGRLLAAGGIYNGNIEGFRETANKLGGDAPAGYDQVMDNK
                     GLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGRPFNPDLAGGPIENLTIDGVT
                     INREGIAIVEKHISRFDHDPANDVMISRLKKIANKELLPEKYDLNYYTHECREYQRYC
                     NLGWETGEPNGLDGYELWNNVHTATLEDFKIKDTDLFHPDAKK"
     gene            1149..1406
                     /locus_tag="J1Z28_001438"
     CDS             1149..1406
                     /locus_tag="J1Z28_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBA7716284.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            complement(1895..4156)
                     /gene="metE"
                     /locus_tag="J1Z28_001439"
     CDS             complement(1895..4156)
                     /gene="metE"
                     /locus_tag="J1Z28_001439"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBA7716285.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLNAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDTLNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            4274..5227
                     /gene="metR"
                     /locus_tag="J1Z28_001440"
     CDS             4274..5227
                     /gene="metR"
                     /locus_tag="J1Z28_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBA7716286.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(5115..6014)
                     /gene="bioP"
                     /locus_tag="J1Z28_001441"
     CDS             complement(5115..6014)
                     /gene="bioP"
                     /locus_tag="J1Z28_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBA7716287.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(6090..6890)
                     /gene="yigL"
                     /locus_tag="J1Z28_001442"
     CDS             complement(6090..6890)
                     /gene="yigL"
                     /locus_tag="J1Z28_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBA7716288.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6898..7920)
                     /gene="pldB"
                     /locus_tag="J1Z28_001443"
     CDS             complement(6898..7920)
                     /gene="pldB"
                     /locus_tag="J1Z28_001443"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBA7716289.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLGDP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            8031..8651
                     /gene="rhtB"
                     /locus_tag="J1Z28_001444"
     CDS             8031..8651
                     /gene="rhtB"
                     /locus_tag="J1Z28_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBA7716290.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8712..9332)
                     /gene="rhtC"
                     /locus_tag="J1Z28_001445"
     CDS             complement(8712..9332)
                     /gene="rhtC"
                     /locus_tag="J1Z28_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBA7716291.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(9396..11225)
                     /gene="recQ"
                     /locus_tag="J1Z28_001446"
     CDS             complement(9396..11225)
                     /gene="recQ"
                     /locus_tag="J1Z28_001446"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBA7716292.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            complement(11350..12219)
                     /gene="pldA"
                     /locus_tag="J1Z28_001447"
     CDS             complement(11350..12219)
                     /gene="pldA"
                     /locus_tag="J1Z28_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBA7716293.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            12384..12851
                     /locus_tag="J1Z28_001448"
     CDS             12384..12851
                     /locus_tag="J1Z28_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBA7716294.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            12903..13790
                     /gene="rarD"
                     /locus_tag="J1Z28_001449"
     CDS             12903..13790
                     /gene="rarD"
                     /locus_tag="J1Z28_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBA7716295.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(13919..14869)
                     /gene="corA"
                     /locus_tag="J1Z28_001450"
     CDS             complement(13919..14869)
                     /gene="corA"
                     /locus_tag="J1Z28_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBA7716296.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            15333..16235
                     /locus_tag="J1Z28_001451"
     CDS             15333..16235
                     /locus_tag="J1Z28_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBA7716297.1"
                     /translation="MKKTFSALFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTANYWPQGSVFVSVVDPGVGTERKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCTK"
     gene            complement(16290..18452)
                     /gene="uvrD"
                     /locus_tag="J1Z28_001452"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(16290..18452)
                     /gene="uvrD"
                     /locus_tag="J1Z28_001452"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBA7716298.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(18536..19252)
                     /gene="yigB"
                     /locus_tag="J1Z28_001453"
     CDS             complement(18536..19252)
                     /gene="yigB"
                     /locus_tag="J1Z28_001453"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBA7716299.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEQEALTFVQNYHP
                     ALRSFQNEDLQRLRQAVWEAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGHYFEFVLRAGPHG
                     RSKPFSDMYLLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(19252..20148)
                     /gene="xerC"
                     /locus_tag="J1Z28_001454"
     CDS             complement(19252..20148)
                     /gene="xerC"
                     /locus_tag="J1Z28_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBA7716300.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(20145..20852)
                     /locus_tag="J1Z28_001455"
     CDS             complement(20145..20852)
                     /locus_tag="J1Z28_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBA7716301.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPDFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNYTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            complement(20849..21673)
                     /gene="dapF"
                     /locus_tag="J1Z28_001456"
     CDS             complement(20849..21673)
                     /gene="dapF"
                     /locus_tag="J1Z28_001456"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBA7716302.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(21710..21913)
                     /locus_tag="J1Z28_001457"
     CDS             complement(21710..21913)
                     /locus_tag="J1Z28_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7716303.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            22102..23571
                     /locus_tag="J1Z28_001458"
     CDS             22102..23571
                     /locus_tag="J1Z28_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBA7716304.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLLAPFGPWDTLARTQTKIMINGEQLDADPDTFSVVRWMPGSLLTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLLEKKVFWRQGPDCKQEELPGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWPNHSGLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVHLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            23568..24888
                     /locus_tag="J1Z28_001459"
                     /pseudo
     CDS             23568..24888
                     /locus_tag="J1Z28_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            24936..25256
                     /gene="cyaY"
                     /locus_tag="J1Z28_001460"
     CDS             24936..25256
                     /gene="cyaY"
                     /locus_tag="J1Z28_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBA7716305.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(25296..27842)
                     /gene="cyaA"
                     /locus_tag="J1Z28_001461"
     CDS             complement(25296..27842)
                     /gene="cyaA"
                     /locus_tag="J1Z28_001461"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBA7716306.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            28229..29170
                     /gene="hemC"
                     /locus_tag="J1Z28_001462"
     CDS             28229..29170
                     /gene="hemC"
                     /locus_tag="J1Z28_001462"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBA7716307.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNTYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGIALAEELLNNGAREILAEVYNGDAPA"
     gene            29167..29907
                     /gene="hemD"
                     /locus_tag="J1Z28_001463"
     CDS             29167..29907
                     /gene="hemD"
                     /locus_tag="J1Z28_001463"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBA7716308.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            29929..31110
                     /gene="hemX"
                     /locus_tag="J1Z28_001464"
     CDS             29929..31110
                     /gene="hemX"
                     /locus_tag="J1Z28_001464"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBA7716309.1"
                     /translation="MTEQEKTSAVVEETREAVGTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPQADAPAAAPQG
                     E"
     gene            31113..32309
                     /gene="hemY"
                     /locus_tag="J1Z28_001465"
     CDS             31113..32309
                     /gene="hemY"
                     /locus_tag="J1Z28_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBA7716310.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
BASE COUNT         8125 a         8618 c         8299 g         7628 t
ORIGIN      
        1 aaagagagtt catcaaagat gagtgatgaa atttacctga ccataaccgg agaacaacaa
       61 ggatgtattt cctctcgttg cggaacgagc gcatcaatag gtaatcgccg gcagattggc
      121 catgaagatg aaatcttcgc gttctcgcta tctaatagta ttacgaatac aggcaaaggg
      181 tcacaattac atggccttag tttctgtaag ttgattgaca aaagctcccc gttacttatt
      241 aacgctatta acaataacga gcagttgttc atggagtttg acttctatcg gatcaataga
      301 tttggtcgat gggagaaata ttacaacatt caactccgcg gcgcgctctt atcagcaatc
      361 aatcacctgt ttactgagaa caatctggat acagaaacca tcactgtcag ttatgaatat
      421 atactctcca agcatctgat tgctaatact gaatttagtt atttggcatt tcccgataac
      481 tacaaccgtc tgtttatacc acggcaaaaa actaaagatg ataatggcct caaaacactc
      541 aatagtaaag cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa
      601 ggattcaggg aaacagcaaa caagttggga ggagatgcac ctgctggtta cgatcaagtt
      661 atggataaca aagggttact catcgctggt gcttcaatag ttgctggagt tgttctggga
      721 agaatgagat tccctgattt ggaatcattt gagcatttcg gagccagagg agcagtatca
      781 ggaagaccat ttaatcccga ccttgccggt ggtccaatag aaaacctgac gatagatggc
      841 gtaacaatca acagggaagg tatcgctatt gttgaaaagc atatctccag atttgatcat
      901 gatccagcta atgatgtaat gatttcaagg ctaaaaaaaa tagcaaataa agaattgctt
      961 cctgaaaaat acgatctgaa ctactatact catgaatgcc gcgagtatca gcgttattgt
     1021 aatttaggat gggaaacagg tgaaccgaat ggtttagatg gatatgaatt atggaataat
     1081 gtacatactg caactctaga agactttaaa attaaagata ctgacttatt tcatccggat
     1141 gcaaaaaaat gacgattacg atagttgaat ttaacgtgct gaaagttatg gctgagaaag
     1201 atattgactg gagctggatg gtgctggatc gtactttagc tatacgaaat attccaggat
     1261 ttggcaacgt agcgaatatc gtaacaaagc tggttaatca tgggttggtt gatatcgtaa
     1321 atggtgaggg caactctaaa ccacgctaca gagtttctca atatggattg aatcttatca
     1381 aagaacaaca agacaacctc ttctgatcca gccattacag gagcatgatt tacattgtga
     1441 tctttcaata agatactcat caattttggt acagaatatc gtgatcacta aaaatgaact
     1501 taacgtgctg aacgttatga ctgagaaaga tataaactgg aactggatga atctcgatcg
     1561 cacactatcc ataaggcaaa ttccaggatt cgggaatgtt gttaacattg ttaacaaatt
     1621 agctaacgaa ggtttggtta ttattgagga tagcgggaat aagtctatgc ctcactacca
     1681 cgttagtgaa aagggataca atctcgtgca acgtgagaat aaataacgta gcgttgatta
     1741 ctgaacggaa tcactcaaaa ctgtgttttg cgaaaaaaac gattcggtta tttctgctcc
     1801 tgaagatctg cgtcaatctg gcactcatgc aaaatggctt ccagtactgg aagccattat
     1861 cgtactgcaa tttacagtga ctcgttaata ttcgtcatcc ccgacgcaaa ttctgcgccg
     1921 cctgcaccat gttcgccagt gccgcgcggg tttctggcca gccgcgcgtt ttcaggccac
     1981 agtccgggtt gacccacaga cgctctgccg gaatgcgttt tgccgctttc ttcagcaaag
     2041 cttcaatcca ttccacgctc ggtacgtttg gcgagtgaat gtcatagacg ccaggaccga
     2101 tttcatttgg ataatcaaac tcttcaaacg actccagcaa ctccatgtcg gaacgcgagg
     2161 tttctatggt gatgacgtct gcgtccagcg ccgcaatcga atccatgatg tcgttgaact
     2221 cgcagtaaca catgtgagtg tggatttgtg tgtcatcttt cgccacggcg gcgttgatac
     2281 ggaaagcctc cacgccccac tggagatagg catcccagtc gctgcgacgc agtggtaaac
     2341 cttcgcgcaa tgccggttcg tcaatctgaa tgatgccaat tccagcggct tccaggtccg
     2401 cgacttcatc acgcagcgcc agcgcaattt gtttggcgat ggtttcacgg ctgacatctt
     2461 cacgcgggaa cgaccagcag agaatagtca ccgggccggt caacatccct ttcaccggtt
     2521 tatcagtcag ggattgcgca tattttgccc actccacggt aatcggtgcc gggcggctaa
     2581 cgtcaccaat aacaatcggt ggcttcacgc agcgggaacc gtagctctgt acccaaccgt
     2641 tttgcgtaaa gacaaagcca tccagatgct cgccaaagta ttccaccatg tcattacgct
     2701 cggcctcgcc atgtaccagc acatccagtc ccaaacgttc ctgctcaaca atggcctgct
     2761 tgatatgttc cgcaatgccc gtgcggtagt tattggcgtc gagattaccc tttttaaaat
     2821 ccagacgcag ggtacgaatc tccgtggttt gcgggaagga accaatcgtg gtggtcggcc
     2881 acgcgggcag tttaaaacgc gcacgctgag cttcagcacg cacttcatag acattcgcac
     2941 gctgactgtc ctgagcggtg atcgccgcca gacgcttttc tactgccgga ttatgtacac
     3001 gagtagagtg acgacgcgcc tgaatcggag cgctccactc tgccagagct gccgtatcac
     3061 cactgttcag cgtatcgcgc agcaatgcca gttcatgaca tttttgcagg gcgaaggcaa
     3121 accagctttt cacttctgca tcaagacgcg tttccacgct caggtcgatg gggctgtgca
     3181 gcaacgagca ggaagatgcc acccacaaat cgcgtttgcc gacaatgtcc ttaatttgcg
     3241 catatttctc ggtaagatcg gcgcgccaga cgttacgacc attgataaga cccgcagaca
     3301 gcagccagtc agaaggcaga cgcttgtgca gttcagcaac gtcatcttta ccatgtacga
     3361 gatcgacatg cagaccctga acaggcagcg cagtaatcgt gtcgaggttt ggcgttacgc
     3421 cttcaaaata ggtggtcagc agcagtttca cctgtccctg gagcgcgtcg taagcgggtt
     3481 tgtatgcgtt cagccacgcc tgcggcagtt ccagtaccaa cgcgggttca tcaatctgta
     3541 cccactcgat gccgcgtttc gccagttctg ccagcacttg ctgataaacc ggcagaatgt
     3601 cgttcagcag gctcaggcga tcaaactgtt cacctttcac tttacccagc cacaggtagg
     3661 taatcggccc cagcagcaca ggtttcacct tgtggcccag cgccagcgcc tcgtccactt
     3721 cctccagcag ctgcgtccag gtcagtttga actgttggcc tttaacgaac tccggcacca
     3781 tgtagtgata gttggtgtta aaccatttgg tcatttccgc tgccgccgca ggttcgccag
     3841 tcggtgcacg tccacgacca atacggaaca gggtgtctat atctaccgaa ccatctttgt
     3901 tctgatgacg tggcggaaca ttacccagca gcagactggt ggtcagtaca tgatcgtacc
     3961 aggcaaaatc gcccaccggc agcaggtcga tacccgcttg cttttgttga tcccagtgac
     4021 gagcacgcaa ttcacgccct accgccagca gttcttcacg cgtggagttc cccgcccaat
     4081 aactctcttg cgcttttttc agctcgcgac gcaggccaac gcgagggaaa ccgagggtgt
     4141 gattaagaat tgtcattata tgccccttat ggaattttta gtatttagcc gtccagatgt
     4201 ttacacatcc ataattggcg gttactgtat attcctcaag cgcaaattat tcatgccgaa
     4261 gtgaaggact ttcatgatcg aagtaaaaca cctgaaaacg ctacaagcgt tgcggaactg
     4321 cggctcactc gcagccgctg cggcgacgtt gcatcagacg caatccgccc tgtctcacca
     4381 gtttagcgat ctggaacaac gccttggctt ccggctattt gtgcgtaaaa gccagccgct
     4441 tcgctttaca ccgcagggag aaatcctgtt gcaactggca aatcaggtgc tgccgcaaat
     4501 tagccaggca ctgcaagcct gcaatgaacc gcagcagacg cgcctgcgca ttgccataga
     4561 gtgccatagc tgtattcagt ggctgacgcc cgcattagaa aatttccata agaactggcc
     4621 gcaggtggag atggatttta aatcgggcgt aacgtttgat ccgcagcctg cgttgcaaca
     4681 gggcgagctg gatctggtga tgacgtccga tattctgccg cgcagtggcc tgcattattc
     4741 gccgatgttc gactatgaag tacgtctggt gttagcacct gaccatccac tggcggcaaa
     4801 aacgcgtatt acaccggaag atctcgccag cgaaacgctg ttgatttatc cggtgcagcg
     4861 tagccgactg gatgtctggc ggcattttct tcagccagca ggcgtcagcc cgtcactgaa
     4921 aagcgtcgat aacaccttat tgttgattca gatggttgcc gcacggatgg gtattgccgc
     4981 actacctcat tgggtggtgg agagttttga acgccagggt ctggtggtca ccaaaaccct
     5041 gggcgaaggc ttgtggagcc gactgtacgc cgccgtgcgc gatggcgagc agcgtcagcc
     5101 gattacagaa gcgtttattc gttcagcgcg caatcacgcc tgcgatcatc tgccgtttgt
     5161 gaagagcgcg gagcgaccca cttacgatgc acccacagtg aggccaggat caccagcgcg
     5221 cctgtaataa acgttggcca gtgcggctgt tggtgccaga tagccaggtt taccagcagc
     5281 cctgccggaa cgtgcatatt attcataatg cccagcgttc cggcgtccac ctgcgtcgcg
     5341 ccgtagttcc acataaagta gccaatcccg gaagccacca cgccaagaaa caccagaatg
     5401 ccccattgca acgtggtttg cggcattttc tgcgcatttc ccagcaagaa ccatgcaatc
     5461 actgccacca ggaacgcgcc aagatagaac cacgcaaagg cgttatgctg cggcatcggg
     5521 cgagtttcca tcagacgttt gtaacccacc atgccgatgg caaaagtgat attggagagc
     5581 tgcaccagca acaagccagt ccagaaatgg tcggtgacct gatcatagcg aataatcccg
     5641 gcaccaatca ccgccaacaa ggcgctaaaa gcatagcccc agcgcagacg gcgcttactc
     5701 atgatgtcat aaatcagcgt gatgtagagc ggcgtcagta cggtgaacaa cagcagttca
     5761 gaaaccgtca ggtagagata ggcgcggaaa ctcagcatat acatcacacc gagctgcatc
     5821 gcgcccacca gcatatacag gccgaccgtt ttcaggctat tgccacgggt acgcagaaac
     5881 ggcagaaaaa cgagtgccgc caggccaacg cgcaccagca ccgcaaaata gctatcgacg
     5941 tgccccgcaa ggtactcgcc ataaaagcta aaggagaagg cccacagaat cgtggtgatg
     6001 ataagtagcg ccacaatgga aaactctttg attaacgagt atccattgta gcgaagtatc
     6061 aggttgacaa ctgaccaaat aaagaacgat tacgataaat agagcttacg cagataatgc
     6121 ggcactgcgt cttcggcgtt agtaccaatc acttccagct ctggatgcag atctttcagc
     6181 cgttgatgcg cgctgcccat aatgcagcct ttccccgcca tcgacagcat ttcggcatcg
     6241 ttcattccat cgccaaaggc aatacaatct tgcaggctgt agcccagttt cttcgccacc
     6301 gcttccagcg catgaccttt tgaaacgccg cccgccatca cttccaggca ggttaaggta
     6361 gagaaactga cattgacgcg atcgccccag cgagcgttaa tcgcctgctc cagtggcaga
     6421 agtttttcat gggaatcgca ggtgaagaac actttgctga cgccttccgg ctccagcaat
     6481 cccggctcat acagcgcata tttgaacacc gcttctttaa aaaagcgcat ctcttccggg
     6541 cgatggcgat tcataaacca ttcgtcgtcg cgataaacgt tagtaatgat gtccggattg
     6601 tcgttgacta cgccaaacag gtcactggca atgtcgcgat ccaggttatg agcaaaaatc
     6661 aggttgccat cgaggtcgtg aacgcgcgcg ccattggagg taatcatgta agacttaatc
     6721 tccagattat cgcgaatttg ccccacatca acgtggtgac gaccggtcgc aaacacaaag
     6781 ttgatgccgc gcgcggtgag cagcttcaga gtttctttgg cgtaagggga taacgtatgg
     6841 tcgggagaaa gtaacgtgcc atctaaatca gacgcaacaa cctggtacat aagaaattta
     6901 aacctctgta gagcggtttc cgctgggtga gttatgcctg ttgaaaaaat caacgatagc
     6961 gtggagcgcg actgagcgca ttgcgtcctt ttcaaaaagg atctcatggt acgcaccttt
     7021 aattaccaac ggccgtcctc cttcgacagg atggcccgca gcggtgcgta gttcacaaaa
     7081 acggtcgtgc atgcggttat ccaccacgcg ttcctcttca gcttgtaaga gaagcgttgg
     7141 cgtggcgtca tcacccgcac cggctaacac ctgttcgcca gccagaatac tttcgcgtac
     7201 ccaatggtag gtcggcccac cgacgcgaat cgttgggtca tcagcataga aacgtaagtt
     7261 acgtcgatat cgctgtctgc tgtgggtcag tacgttgata gcaaacggca acgcgcgcca
     7321 gcgcccggtg cctatcgcat agccatcacg gaaacgtgga tgcgcttcgg cccagttaag
     7381 gatctgccgt gccataaatg acggcatacg aatcacaatc ccaaacattg gcgcagttag
     7441 cgcaatggcg tcacatacac ctggatggcg ttgcagaaat aatgtggaga tcgcaccgcc
     7501 catcgaatgt gccagtatat agcgtttacg ccacggaccg ggctgaacct cctgttgcca
     7561 gaatgccgcc agatcatcaa cataatcatt aaagcgatta acatgcccga gatgtggatc
     7621 gcctaacagg cgaccggaac gtccctgccc gcgatggtcg atgattaaaa catcaaaccc
     7681 caaatggaac aggtcgtagg ccagttccgc atattttacg tagctctcaa tacgccccgg
     7741 acagatgact accacccggt catggtgctg tgcgcgaaaa cggacaaagc gaaccggaat
     7801 gtcatccaca ccagtaaact ctgcttcatc acgctgacgc cagaaatcag tcagcggtcc
     7861 catggtaaaa gcagcaaacg cgttttctct tgtttcccag tctttttgct gctgaaacat
     7921 cgggtaatct gcctcttaaa ccacgtaaaa tcgttttttt aagcgtgcct gacacaacgc
     7981 tgcgacagta gcgtattgtg gcacaaaaat agacacaccg ggagttcatc atgaccttag
     8041 aatggtggtt tgcctacctg ctgacatcga tcattttaag cctgtcgcca ggctctggcg
     8101 caatcaacac catgaccacc tcgctcaacc acggttatcg cggcgcggtg gcgtctattg
     8161 ctgggcttca gaccggactg gcgattcata ttgtgctggt tggcgtgggg ttggggacgc
     8221 tattttcccg ctcagtgatt gcgtttgaag tgttgaagtg ggcaggcgca gcttacttga
     8281 tttggctggg aatacagcaa tggcgcgccg ctggcgcaat tgatcttaaa tcgctggcct
     8341 ccactcaatc gcgtcgacat ttgttccagc gcgcagtttt tgtcaatctc accaatccca
     8401 aaagtattgt gtttctggcg gcgctatttc cgcaattcat catgccgcaa cagccgcagc
     8461 tgatgcagta tatcgtgctc ggcgtcacca ctattgtggt cgatattatt gtgatgatcg
     8521 gttacgccac ccttgctcaa cggattgctc tatggattaa aggaccaaag cagatgaagg
     8581 cgctgaataa gattttcggc tcgttattta tgctggtggg agcgctgtta gcatcggcga
     8641 ggcatgcgtg aaaataatgt cggatgcggc gtaaacgcct tatccgactt actctgatga
     8701 cgcgtctggc attaacgcga aataatcaaa tgaatgccaa atccggcaaa taacgccccg
     8761 gcaaaaccat caatccactt cgccagacgt tgataaccac ggcgcatttg cggcagagca
     8821 aacaggctgg caacgacggt aaaccacgcc agtgtttcga caataatcaa cgcgaaaatg
     8881 ccccagcgtt cggtggtgcc gacattatca ccgacaaaca gcgagaacac cgaaccaaag
     8941 tagataatcg ctttagggtt cgcgagattg gtcagtaagc ccttaagaaa actgcgcccg
     9001 cttttcgcca gttcgacttg tggcgcaggg gcagaaacaa cctctttttt cagtgcacca
     9061 cgtagcatct ggtaacccat ccagcagaga tacaggccac cgcccaccat aatcagcgta
     9121 tgcagccagg ccattttttc gataatcaaa tgtaggccaa gcaacgcaat cccagcccaa
     9181 accattacgc cgcaggtaat gcccagcacg cccatcatcg cttctttacg ggaacgacta
     9241 acagcggtct gagagacaaa aaagaaatcg ggaccggggc tcataagcgc cacaatgtgc
     9301 accatggcga cggtgaggaa taacatcaac atactttaga ctcgcggggg aaacgtttac
     9361 ggagtcttca tcctggcact tttttatgct gctgactact cttcgtcgtc accatcgaca
     9421 tgcgcacgaa tcagcgccat aaacggtttg ccaaagcgtt ccagcttgcg cataccaacg
     9481 ccgttaacgc tgagcatttc gctggcggtg atcggcatct gctccgccat ctcaatcaag
     9541 gttgcgtcgt taaacaccac gtacggcggg acattgcttt catcggcaat cgatttacgc
     9601 agtttgcgta atttggcgaa cagtttgcga tcatagttgc cgccgaacga tttctgcatc
     9661 gctttcggtt tgagcgccac gatacgcggc acggcaagtt gcaaagagga ttcgccgcgc
     9721 agcaccggac gcgcagcctc tgtcagttgt agggcagaat gctgggcaat attttgcgtc
     9781 accaggccga ggtgaatcag ctggcggatc acgctcaccc aatgttcatg gcttttatca
     9841 cggcccatgc catagacttt cagtttgtca tgaccatagt cgcggatacg ctgattatta
     9901 gcaccgcgaa tcacttccac cacataaccc atcccaaacc gctgattcac acgaccaatg
     9961 gtggaaaggg caatctgcgc atcggttgaa ccatcgtact gttttggcgg atcgaggcag
    10021 atatcgcagt tcccgcacgg ctcctgacgc ccttcgccaa aatagttcag caataccaaa
    10081 cgacgacaag tttgcgcttc ggcaaacgcg cccatcgcat tgagtttatg acgttcgata
    10141 tcctgcaact gcccctgcgg cttctcttcc agacaacggc gcagccacgc catatcagcc
    10201 ggatcgtaaa acagcatcgc ttccgcgggc aggccatcac gcccggcgcg tccagtttcc
    10261 tgataatagg attcgatatt gcgcggaata tcaaaatgca ccacgaagcg aacgttaggc
    10321 ttgttgatgc ccatgccgaa cgccaccgtc gccaccacaa tttgcaggtc atcgcgctgg
    10381 aatttttcct gcacatcagc gcgaacatta ttttccagcc cggcatgata ggccgccgcg
    10441 ctaattccct tgctttgcag gcgcgcagcg gtgtcttcta ctttcgcacg actattacag
    10501 taaataatcc ccgacttacc gcgttgttcc tgtacataac gcatcaactg atcgagcggt
    10561 ttgaatttct ccatcagcat gtagcgaata ttcggacggt caaaactgct gatttgaatc
    10621 agcggatcgt tcagccccag caggcgcacg atatcctggc gcgtggtgtc atcggctgtg
    10681 gcagtcagcg ccataaacgg gagcgtcgga aaacgctgac gcagctgacc gagggcagcg
    10741 tattccgggc ggaaatcgtg accccattgg gagatacagt gcgcttcatc aacggctaat
    10801 aacaccggat tccagtgcgc cagatgctca agaaagttat ccagcatcag gcgttccggc
    10861 gcgatataaa gcaggctgat ttgcccggtg cgacagcctg tcatcacttc aagctgctgc
    10921 tcgcgggttt gcgtcgagtt aaggcacgcc gccgccacgc cgttggcttg cagttgatct
    10981 acctgatctt tcatcaacga aatcagcggt gagacaacca cggtaaggcc gtttagcaat
    11041 aaggcaggga tttgatagca aagggatttt ccgccaccag tgggcatgac gaccaggcaa
    11101 tcgcgaccgg aaagtacggt gtcgataatc tcttcctggc cggggcgaaa ctgttggtag
    11161 ccaaaggttt cttgtaaaac ctgtttagct ccggactcca gattcaacac ttccgcctgc
    11221 gccacattca ccccgattgc ctgaaataaa aacaggcgct attttcagcg cctgcgcgag
    11281 aaactgcaat gcttttagtc tctgagaggg taatcctgga tgctggattc cagatttgcc
    11341 aaccattcat caaaacaggt cgtttagcat aactcccaca ccaacgcggg tctggttgaa
    11401 gttatagtcg atgagcgatt cgccgtaacc gctgtaaacc tgagtataaa ggcgcacatg
    11461 tttggtgatc gggtagctta agcctaactc cgcgccgccg taaccggtgt tccagttgta
    11521 ctgccctttc gcgctcagta ccgcatcacc gaggtgatag ccgattttaa gctggtagta
    11581 acccatatat ttggtgatgt ccgggttatc gtccgtattc cccaccacat accacggctt
    11641 cacctctacc agccagttac cgttttctgc catcaggcgg gtataaaggc ggttccagct
    11701 gcgggaagtc gggtctgaac gcccgtttga gtcgtggttg tagcccattt ccacatcgcg
    11761 cagcgtccag ccagcaaagt tgtaatccgt ggcaaaaccg aggaacaatt gcggttcgta
    11821 gttagtttca cgaaacggtg atgactcatc gctattagaa agctgccacc aggatttctg
    11881 tgtgtaggag gcgcccagta cagagtttgg ccccaaaatc ccacgccaca gcggaaacgc
    11941 caggctcaac tgaaacttta cttcatcctt acgcgcattt tccgcccagt cgtaactggc
    12001 aatcgcttct ttattcagat cgctggtttg ggtgtagatg atgtagttgg tgtcataagg
    12061 ataaagggtg aacggattgt catgctcctg cagcatattg gcgataatac tgccacgcac
    12121 cgctggcgcg tcatgcacct ctttcaccgt tgcctcttgt gcatatactg ccataggcaa
    12181 cataaacacc ggcaacaacc agccctgcag agtccgcatc gctcgtgttc tccgtaacga
    12241 attattgata aggtgaatat ttttctgcgg gctattttac atacttaact ccatagccgt
    12301 tagttatccc ttcctaaagt agaaaccaac aataaataag cataaaatca acatttcatt
    12361 aactaatgaa gtgtaaggat gttatgtctg ccgtactgac cgctgaacaa gccctcaaat
    12421 tagtgggtga gatgtttgtt tatcacatgc catttaaccg cgcattgggg atggaactgg
    12481 agcgttacga aaaagagttc gcacagctgg cctttaaaaa tcagccgatg atggtgggca
    12541 actgggcgca aagcatttta cacggcgggg tcattgcgtc ggcgctggat gtcgccgcag
    12601 gtctggtgtg cgtgggaagt accttaaccc gccacgaaac catcagtgaa gatgaactac
    12661 gccagcggct atcgcggatg gggaccattg atcttcgcgt cgattatctg cgcccaggca
    12721 gaggcgagcg ttttactgct accagtagcc tgttacgtgc aggcaataaa gtcgccgtcg
    12781 cccgcgttga attacacaat gaagaacagc tttatattgc cagtgccacc gccacctata
    12841 tggtaggttg aagacataaa agcaggtaaa ctgctgttac caattaaaaa tgagtattcc
    12901 cgatggatgc aaaacaaacg cggcagggcg tattactcgc tcttgccgct tattttattt
    12961 ggggtattgc gccagcgtac ttcaaactga tttactacgt gcccgccgat gagatcctga
    13021 cgcatcgcgt gatctggtcg tttttcttta tggtggtgct gatgagcatt tgccgccagt
    13081 ggtcctattt aaaaacgctg attcagacgc cacagaaaat ttttatgctg gcagtctctg
    13141 ccgtgctgat tggcggcaac tggctactgt ttatctgggc ggtgaacaat caccatatgc
    13201 tggaagcgag ccttggttac tttattaacc cgctggtgaa cattgtgctg gggatgattt
    13261 tcctcggcga gcgattccgc cggatgcaat ggctggcggt gattctggcg atatgtggag
    13321 tgctggttca gttgtggact tttggttcac tgccgatcat cgcgctgggg ctggcgttta
    13381 gttttgcttt ctacggcctg gtgcgcaaga agattgctgt tgaagcgcaa accggcatgt
    13441 taatcgaaac tatgtggttg ctgcccgtgg cggcgattta cctgtttgct attgccgaca
    13501 gctcaaccag tcatatgggg caaaacccga tgtcgctgaa tttactgctg atcgccgccg
    13561 gtattgtcac caccgtgcca ctgttgtgtt ttactgcagc ggcgacacgc ctgcgtctct
    13621 caacgttagg ctttttccag tacattggtc cgacgctgat gttcctgctg gctgtgacgt
    13681 tttatggtga aaaaccgggt gccgataaga tggtaacttt cgcctttatt tgggtggcgc
    13741 tggcaatttt tgtgatggat gcgatttata cgcagcgtag aaaaagttaa gcattaacat
    13801 gggtaggccg tttaaagagc ctacccacta acttatctat ctttgtatgt tatctgcgat
    13861 accctcggct aaaatgtcgc tgtcagcaca ctaacttact cacagcgaca tcgtaaagtt
    13921 acagccagtt cttccgctta aagtacagat acggtgccag gcccgcgagg atcataaaga
    13981 taatcgcacc tgggtagccg aagctccact tcagttctgg cataaactca aagttcatcc
    14041 cgtagctgga agctaccagc gtcggtggca ggaacacgac ggataccacc gagaagattt
    14101 tgatgatgcg gttctgctcg atgttgataa aacccatcgc cgcctgcatc aggaagttca
    14161 ccttctggaa tagagattcg ttatgcggca gcagggattc gatatctcgc aggatttcac
    14221 gcgcctgctc cagttgcccg cccggtaaac gcgccttacg caccaggaag ttcagcgcac
    14281 gctgggtatc catcagacac aggcgaactt tccagccgat atcttccagt tccgccagcg
    14341 tggagagcgc ctcgtcgtac tcatcgccct gatgcccttc cataattacc cggctcaact
    14401 gctccaggtc gctgtagata ttttcaattt catctgccaa ctgttcgatt ttggtttcga
    14461 acagatccag cagcagctcg taggcgttac cgtctaccat cgactggcta cgggcacgca
    14521 tacggtaaag gcgaaaagca ggcaattcac gctcacgcag agtaaacaga cgaccatcac
    14581 ggatggtaaa tgccacggtg gagttaccgg cgtgatcttc cgcatcttca aagaagaaga
    14641 aggagtgaat atgcaggccg tcgtcgtctt caaagaaacg tgccgatgct tcgatgtctt
    14701 ccagttccgg gcgggttgcc aggctctggc cgagttcaga ttgtacgcgc agtcgctcgt
    14761 cgtcgtccgg ttcgacaaga tcaatccata ctgcatttac aaggggttgt gactcttcga
    14821 cttccagccg ggtcagtcgg ttattttcca gttgaaatgc gctcagcatg accgggactc
    14881 ccaatgctta aaatatcgga cagttcggtg ggcacacaga aacaaattgg gtttcagacc
    14941 attaaacagc ctgactcagc gcgacgggaa aaattgaggt cgctgacaac cgctaaggct
    15001 atcagcaaaa agggatagcc ttaggagttg atcctggatg acaggatagt gagccagtat
    15061 ctactgggtg tgtccaaggc gaatgtcctc ttagagtgat cgtgcgcgca tgttacgcca
    15121 gcaaaatttt gccgtcaaca cgcaacggcg acacattttt ttcctgcttc ctcctgttta
    15181 cgttccccgg aacacttaaa ttttgtggtt aaaatctgaa tccatcaggc gtgctctggg
    15241 taaacaaaag agagagcgtt aaactgataa gttccagatg ctcttcgtct ggcacaacac
    15301 aggacaacat cagataactt aaggaaatat agatgaaaaa aacgttttca gcgttgtttt
    15361 tattaacgtg tctggggtca tcatccgctt atgcggataa cgctttgatt ttacaaacgg
    15421 attttagcct gaaagatggt gccgtctcgg cgatgaaagg ggtcgctttc ggcgttgacc
    15481 ataatcttaa aatatttgat ttaacacacg aaattccacc gtataacatc tgggaaggcg
    15541 cttaccgcct gtatcagact gccaattact ggccgcaagg ttcggtgttt gttagcgtgg
    15601 tcgatcctgg tgttggtaca gagcgtaaat cggtagtgct gaaaaccaaa aatggtcagt
    15661 acttcgtttc accagataac ggtacgctca cgttggtggc ggaatcgctg ggtattgaga
    15721 gcgtccggca aatcgatgaa aaaaccaacc gtctgaaagg ttccgaaaag tcatatacct
    15781 tccacggtcg tgacgtgtat gcctacaccg gtgcacgtct ggcttccggg gcgatcacgt
    15841 tcgagcaggt ggggccagag cttcctgcaa aagttgtcga actttcttat cagaaagcca
    15901 aagcaacaaa aggtgaagtg aagggcaata tcccgattct cgatattcag tacggcaacg
    15961 tctggagcaa tatcagcgac gagttgctga atcaggcagg aatcaaactc aacgacacgt
    16021 tgtgtgtgac catttccgaa ggttcccagc aaaaatacgc agggaaaatg ccgtatgtcg
    16081 caagcttcgg cgatgtgccg gaaggccagc cgatggtgta tctgaacagc ttattgaatg
    16141 tttccgtggc gctgaatatg gataatttcg cgcagaaaca tcaggttgcg tctggtgctg
    16201 actggaatat tgatgtgaag aagtgcacta agtaagttac tgatgccgga tgcggtgtgg
    16261 cgcaccgcat ctggcaacgg aatcgtaaat tataccgtct ccagtcgggc gtaagccgcc
    16321 accagccatt taataccctg gccctggaat gccacctgca aacggctatg ctcgccgctg
    16381 ccttccatat tgacgatggt gccttcacca aacttagcgt ggcgtacgcg ctggccgagc
    16441 ttatagccgc tgtcgttctc gaccatcggc gtacccatac gctgatggct gaccgggcgg
    16501 cttaccgtgg cgcgcaggcg cacctcttcc acacactctt ccggcaactc accgataaag
    16561 cgcgacgggc gatggtaaac ctctttgcca tacagacggc gggtttccgc gtaggtcagc
    16621 gtcagtttct gcatcgcgcg ggttacgcca acgtaggcca gacggcgttc ttcttccaga
    16681 cgcccgcctt catccagcga catctggctt gggaacatgc cctcttccat accaacgata
    16741 aacacctgcg ggaattccag acctttcgcc gagtgtagcg tcatcaactg caccgcatcc
    16801 tgccaggtat ccgcctgccc ctcgcccgct tccagcgccg catgggagag gaatgcctgc
    16861 agcggcatta aatcttcgtc ttcttcgttg tagctgaact ggcgcgttgc cgtcaccagt
    16921 tcctctaagt tttcgatacg cgtctgacct ttttcgccct tctcctgctc gtacatggtg
    16981 cgcaggccag agtctttaat tacccggtca gtctgtacat gcagcggcat atcggcagtt
    17041 tcctgcgcta aggcgtcgat cagctccata aaccgctgta aggcgctggc agcacgtccg
    17101 gcgagggctt tttcctgcaa cagttcacga catgcctgcc agagtgttaa ctggcgatct
    17161 cgcgatgtct gacgtaccac gtccagcgtc cggtcaccaa taccccgcgt tggcgtattc
    17221 accacacgct caaaggccgc gtcgtcgttg cggttggcaa tcaggcgcag atacgagagc
    17281 gcatctttga tttcctggcg ttcgaagaag cgcatcccgc cgtaaatacg atacggcata
    17341 ctcgcctgca ataacgcctc ttccagcaca cgcgactggg cgttgctgcg gtagagaatg
    17401 gcgcactcgg caagcgcccc gccgttgtcc tgccaggttt tgatgcggtt aaccacaaaa
    17461 cgcgcttcgt cgagttcgtt aaaagcgcaa tagagggaaa taggctcacc gtccgcgcca
    17521 tcggtccaca gttttttacc cagacgcccg ttattgtttt caatcagggc gttagcggcg
    17581 ctcagaatat tgctggtaga gcggtagttc tgctccagac ggatagtttc agcaccgggg
    17641 aaatcattga ggaaacgctg aatattctcg acctgcgccc cgcgccagcc gtagattgac
    17701 tggtcgtcat cgccaacgat catcactttg ccagtatcgc ctgccagcag tcggatccac
    17761 gcgtactgaa tgttgttagt gtcctggaat tcatccacca ggatattggt gaaacgttcg
    17821 cggtaatgtt gaaggatatg cggtttgttg agccataatt cgtgggcacg aagcagcaat
    17881 tcagcaaaat caaccagacc cgcgcggtca caggcttcct gatacgcctg atacaccttc
    17941 tgccaggttt gctccactgg gttgccgtag ctctgaatat gatgcggacg cagcccctca
    18001 tctttctggc tgttgatgta ccacattgcc tgacgcggcg gccactgctt ctcgtcgaga
    18061 ttcatcgcct tgatcaaacg cttaagcaga cgtagctggt cttcactgtc gaggatctgg
    18121 aaatcctgcg gcaggttggc gtccatatgg tgcgcacgca gcaggcggtg cgccagcccg
    18181 tggaaggtgc cgacccacat accgccctgg ctagtgccca tcagttgccc gatacgatga
    18241 cgcatctccg ccgccgcttt gttggtaaac gtcaccgcca taatcgaata cggcgagcag
    18301 ttttccacgc tcatcaacca ggcgatacga tgcaccagta cgcgcgtctt accactgccc
    18361 gcgcccgcca gcaccagaag gttgctgcgt ggcgcggcca ccgcttcgcg ctgtttgtca
    18421 ttaaggctgt cgagcaggta agaaacgtcc attggcaccg ccgcgtaaaa ataggttggg
    18481 cggagaagcg caacgccctc cgccaaaaag ctgggtatat atacagattt gctgattata
    18541 tcagcgaggt cagagatgcc aaccgggaaa tttccagatg cggcagtaaa cggctgtccc
    18601 aggtttgcat cagatcgcca ttttccggtc tgatccaaca ggcctgcatt ccgctgcgaa
    18661 ttgccccacc cacgtcagtg gtgagatcgt ccccgacatg taagatctca ccgatcggca
    18721 cgttaagttt ttccgccgcc agtaagtaca tgtcgctgaa cggttttgag cgcccgtgcg
    18781 ggccagcgcg cagcacaaac tcaaaataat gccccaaacc aaacagttcc ggctgggcgt
    18841 taccgttggt gatcgccacc agcggccatt tcttcgccag ctgttttaag gtgtcgtggg
    18901 tttgctgcgg gacgtcgatc cggctgcgcc atttggcaaa gttgatcatt gccgcgtgtg
    18961 cgcctgcact ggcttcttcg gcactcagcc cggcgtcgag catcgcttgt tcaatcgaac
    19021 gaaaacgcca gcgcgtcacg tcgtgataaa tctcgggttc cgcttcccat accgcctggc
    19081 gcaggcgttg cagatcttca ttctggaagc tgcgcagcgc cggatgataa ttttgcacaa
    19141 aggtaagcgc ctcctgctcg gtgcgcaaaa tcaccggacg gttatcgtaa agggtgtcat
    19201 ccaggtcaaa ggtgagcgcc gagatgcgcc ccaaaggccg gtaaaaacgc attatttccc
    19261 ccgtttggcg cgtggatgcg ccgcatcgta caccgaggca aggtgttgaa aatcaagatg
    19321 agtatagatt tgcgtggtgg agaggttggc atgacccagc agctcctgca caccacgaag
    19381 atcgccgctc gactccagca tatgggtagc gaacgagtga cgtaatttat gcgggtgaac
    19441 gtgattattc agcccttgtt ttatgcccca ttcggcaaag cgtttctgca cattacgcgc
    19501 ggagatacgc ttgcccagtt tcgacagaaa aagcgcgtcg tcttcgctac caaacaggtc
    19561 gcgcaaatca agccagtgct caatccacgc cacagcgttg cgaccaatcg gcaggcggcg
    19621 ctctttgctg ccttttccca tcacccacac ttcaccagac tccaggtcga ggtgtttgat
    19681 atccagcccc accagctcgg aaagacgcag acccgcgccg tacatcactt ccagcattgc
    19741 acggtcgcgt acagcgaggg gatcgttgat atcaatatcc agcagccgat tcatatcatc
    19801 gacgtcgatg tttttcggca gatgacgcgg cgctttcggt gccgaaacac ctttcgccgg
    19861 gttggctttg agttcgttct ggctgaccag ccagtcaaaa aagctacgta gcgcagaaag
    19921 ccgtaacgcc agacttgcag ctcccagccc tttacggcga ctgcgtacag caaaattacg
    19981 caccatcgtc acatcacatt gctgccagct ttgcaggcca ttttcgctgg caaaattgat
    20041 gatcgcctca agctgtcgct ggtagttaag cagggttatc gggctaagct ggcgctccac
    20101 gctcagataa cgtaggtagc gttctacatc ggtgtgtaaa tcggtcatac gcgttcaatc
    20161 caacgcgcca gaagctccgg caacatcagt gcaatttcat gaagtaactg cgttccctgc
    20221 ccttgttgat agtgactggc atcgcgactg gtaaacagca caacacccaa atcagcatcg
    20281 cttcccagca tcgacatcgc caccgatccc accgctttcg cttccggtag caccaccagc
    20341 agctccggtc cgttaagcgg tcccagatag tgctgttcct gccccagacg ctgaatacgc
    20401 agcggttcga aagactgacg gcttaatgcc agatgagtgt agttcgacgg cgcacctaag
    20461 cgccagcgat ccggaaacag gcgcagactc gcacctgcca ggccgagatc gcgcgcccag
    20521 cggtgaaagc gcatcagcat atcatcgaga ctgctggcgg cggtgagact gcgctgcagg
    20581 tagagcagac gataaaacag gccttcgttg gcgattgcct gttccatcaa cagcgccatg
    20641 ttctcttcca gaacatgaat atgattacgt gcgcgggcca tgtgccactc gaccaacgaa
    20701 acggtgccgc gtaccggatg cggcacacgt atcgcttcta ctgcgcgcgc attgcggata
    20761 aaaaaatcag gatttttaat cagataatcg acaaccgccc ggtcatcaag ctccgtgagt
    20821 gtttcctgca gttcttcccc tggttgcttc atagatgaat aaatccgtcg tagacatgta
    20881 ccgccgggcc agtcatatat aacggatgac ccggaccttt ccaggcgata tcaagacggc
    20941 caccagggag ttccacacgt acttcttcgg ccagcaaacc ttgctgaatc cctaccgcaa
    21001 ccgccgcaca cgcgccgctg ccgcaggcct gggtttctcc tgccccacgc tcataaacgc
    21061 gtaaacgaat atgctcgcgc ttaaccactt gcataaaacc gatattggcg cgctccggaa
    21121 aacgttcgtg gctttccaga acagggccta gcgtttctac cgccgcggta tcgacatcat
    21181 cgacctgaat cacgcagtgc ggatttccca tcgacaccac gccacataag attgtctgct
    21241 cggcggcgcg cataatatag gtcttttccg ctttgttagc gcgaaacggc acggcggaag
    21301 gttcgaagtt gggttcgccc atatttacgc ggaccagatc atcatcggtg acggtcagaa
    21361 ccatccgccc gttggcggtg ctgacgcgga tatcgcgctt attggtcagc cctttcaggc
    21421 gcacaaaacg ggcaaagcag cgcgcgccgt tgccgcactg cgccacttca ctgccatcag
    21481 cattaaaaat gcgatagtga aaatccagtt caggatcata cggcggctca accaccagca
    21541 actgatcaaa ccctaccccc aggtgccgat ccgccaggcg acgaatcagc tccggtgaaa
    21601 aaaagacatt ctgcgttacc gcgtcgacga ccataaaatc gttgccaagg ccatgcattt
    21661 tcgagaactg catcatttac tccaatcacg cgggtacaga aactgacttt tagtaattca
    21721 cctgggatgg accatcgcca gtggcgcggt cgtttttatc cggcaccgtg gattgcgttt
    21781 gcgtctctac cggtttggtc ggcggcggtg cgtttttatc tgcaggcggg aaatagagcg
    21841 gacctttcag accgcagccc gtcaggctga agagagtaag taatacagtg agtgccttaa
    21901 acacgttttt cattatggat tgcctgtaaa gttcatcgct ttctgctttc tatcatcgca
    21961 ggagaaggcg taaaagcaag cgtttagcga ctctttatga agtctgaaaa gtgaggaaat
    22021 gctgcgagta taacgcttac tcgtggcgat ttatgttttg ctggtagttt cgtgatcggc
    22081 acctcgcgcc aggatggctt tatgcactat cgcaatgtgg tttgtttatt gtcttgttcg
    22141 ttgttcttat catcggcatg gggatgtcgg cttgatgagc cggaacataa tatttaccag
    22201 aaacagggaa aaggcgtggt gtacctgcgc ccttatgaaa agaccaatct ttcgctaccg
    22261 cagattaact acaagcgtct gcgtctgtta ccgaatttgc taattgaccc aaccaaactg
    22321 gaggattggg aaacggtgcc cccagctacc aatctcatga cagacgttgt ctacagcggc
    22381 gcaaacgcaa ctcttcccca ttactcttac tatagcgatg gtcgcgctat tctctacgct
    22441 ggcgagattg tgcaaaaccc tccaggcaca ccgccagtcg atatctcgtc atttcaggca
    22501 tggggtgatt ttgccgcaga taagtacagc ctctattacg aaggcaaacg caccgatagc
    22561 aaccagcaac taaaccgccg aacgttgcgt caggtagaat ttaacccgca atggaaacca
    22621 gactggctag gtttgattct ccgtgacaag cactatcttt acgcaaatgg tcagcgcctt
    22681 gatgatcccg acacctttac ggtactggct caaaagtcat gggatcagcg cggtaaattc
    22741 tctacagcat ttaatccctg ccttcttgcc ccatttggcc cctgggatac gctagcccgc
    22801 acacagacga aaatcatgat caacggagaa caactggatg ccgacccgga caccttttcc
    22861 gtcgtgcgct ggatgcccgg ctcactcttg acctggcgtg ataaaaacgg gttacagcgt
    22921 aaagtcctcg acaaggaaaa tctggcgtgg gatgaggatc taacaaagca ctgtctggat
    22981 ttttctctgc tggaaaagaa agtgttctgg cgtcaggggc ctgactgtaa acaggaagaa
    23041 ttaccgggac tcgatcctga acagttccac cccatcagtg atgctgtcgc ccagtatcag
    23101 gattcgcttt ataccatcat cgaaacagag tctggtgacc gcaagttgga gatcgtaaaa
    23161 ctcgatgacc ccaatcttat tatcaacaaa cgtttcaacg ccgggaaacg ccacggctat
    23221 ttacttacgc gtgccgaagg gtggccaaac cattccggtt tacacgtgtt tgaatctgac
    23281 ggaccgctga tcttactgga taaccgctct ccggatgaac gcgaagcaca tcttaatgac
    23341 catccctttt tgcgcagatg gtatgcccgc gataaccgct acgtttacag ctttgatggc
    23401 gcgcagctct ggcgataccg caccgctgat ccgaaacaag ttcacttaat ctggaaggaa
    23461 caacattcgg gatatggcta tggcgtaaat tacaaaacgg gatatctgga cggaaaaatt
    23521 accgatgacg gcgaatttat tcctgcccca cgcaatgagg caacaaaatg aatggtcaaa
    23581 taatcacagt tgcactcatc ctgttgggga acacatttct gacacctcct gtacaggcca
    23641 tagggttcga ctattacaat gatcacggtg tcatgtctta cggtaaaggt tattgggggg
    23701 ataagaaaat cattgcccaa tttccggaga tgaacagggc cgatttgcgc cttgtgaaaa
    23761 atatctctgg gaagacgggt tatatacctt ccgatgaaat aagcatagaa aatgaggaat
    23821 atcactgggt gacagacggc cacgttattc tctggcgtgg caaaatcgtt agcaatccac
    23881 cgggaacacc cactgtcgat attgccagct ttcaggctat gggccgcttc gcggtcgata
    23941 aatatagcct ctattttgac ggacagcgta ccgaaagtaa tagcggcgcg tcacatgttg
    24001 atctggcgac actaaaagct atcgaaggta actctaccac gctggtggat agtaaaaatc
    24061 tctacttgtc cggtcgacgt caggggagca gcagtaatgt tactgtatta gaaaaaagat
    24121 ggtggggtat taatccacgt cttatgagtg taaacagaaa tttgtattcc aatgatctgc
    24181 ttatccgcag tgggcagaat atttatttaa atggcgctca ccttacggcg aatgcagact
    24241 catttgagat aattcgctgg atacctcatt cactgctggt ttttcgcgac aataagggtc
    24301 tgtctgcatc gttatccctt tggtcaatta tctggcaaag cgatatccgt agatgatgac
    24361 gtctcttttg aagtagggga aagtcgcgtt cgctggcgta aacagctcac gcccgaccgt
    24421 cagtggagca agtggataga cctaccgggt attgaacctg aacaatttca tctgattact
    24481 gacaatattg cgcagtataa agatcgactg tatgtaacaa aattatcgac ttttggtgaa
    24541 gaccagcttg agataatccc gctggatacg ccagacctgg tcattgatga ctcatttaat
    24601 agcggcaaac aacatgctta ctttatccgc caattacggt cacgcaagag cgtgcaaatt
    24661 attccagtta acggtccgct aactaaaaac gatcgcttcg cttatgacga tcgcaatgtt
    24721 tatacctgga ctgatacaga ggtaaggatt acgccctccc actgcccggc gaaaacacgc
    24781 gtcagagaag agaatgtccg tgaaattcat aacagcgaca ttattattcc ggtgacggat
    24841 gaatcatgcc ggaacgcagc agcagacggg caaactttga agccctgacc ccatgccgct
    24901 atactgccgc catcacttaa aaacaggata caacaatgaa cgacagtgaa tttcatcgcc
    24961 tggctgatca actgtggctg accattgaag aacatctgga cgactgggat ggcgacagcg
    25021 atatcgactg tgaaatcaac ggcggtgtac taaccattac ctttgagaac ggcagcaaaa
    25081 tcattatcaa ccgccaggaa ccactgcacc aggtgtggct agcaaccaaa cagggtggct
    25141 accattttga tctgaaaggc gacgagtgga tttgcgatcg tagcggtgaa accttctggg
    25201 atttgctgga acaggcggcg acgcagcagg cgggtgaaac agttagtttc cgctaagatt
    25261 gcatgccgga taagcctcgc tttccggcac gttcatcacg aaaaatactg ctgcaacagc
    25321 ggcgcgtcat gctcctgatt ggcaggtggc atgttaccga tagatttggt gcgaaacgga
    25381 atcacctgtt cacgaccatc aaccttcaca atctgataga actgcggcag gttgaagttg
    25441 atgaagcttg agccgtaggt gaaacggtcg tgcgaagacg agtagaagcg actgacgtca
    25501 cgcaccagtt cctctttgct gccttcgcag tggtgataca cctcaacccg gttgctttcg
    25561 tcgagaatgt agatattaaa gccattctcg tcttgcgttt cttcgaagaa aaactggatg
    25621 atcccttcgc tggcaaagcc gtccaccacc gccggtaatt tgacgtgatt ggtttcaacc
    25681 tgcactgaca ggccgtgcag tttgttatgc gaaatagcgc cataaaactc gatggcgttt
    25741 tccagtttct gtaccgagac attcagccgt tcgaagaaca agccccaggt ttgaccggag
    25801 acgcgcagcg ccttgaagcg cccggtttcc tggcgggtgc tggaaagacg cagttcaata
    25861 cactcagaaa ccagttgctg cacgcgagta cggattaagc cgcgcagatg ctggctataa
    25921 cagaagactt ccacgctatc tggcggtgcg gcgtcctgat gcattttgcc gagaatagtt
    25981 ttcagggctt cgatcatcga ttgctcgccg ttgaagtgca gcgtacgcac ttcgttccac
    26041 gagttgcgat acagcaggtc aacgctacct accaggcaat tttgattctc gccaaagctg
    26101 aagacatcca gcttacggaa atcaaaatgc accacctgat tgcggaacgc agctgtcggg
    26161 tcatattcca ggttaacgat aatcgccaga tggcggatct cacacgggct gtagagcgcc
    26221 ttcggtgtcg gtgcaggtaa gcgcagcggg aaatggtgcg acacgtcggc gaccatctcc
    26281 tgcaacttag gcaaatcgac aatgccgtta cctttaatat acaagcgggt gcgcgaggtc
    26341 agcaggccgt taaaccatgc ccacgccacc agtttattca ggtaacggtt atattccagc
    26401 ggctgatggc tgatgatcga ctcaatattt ggcgcgcggt tatacagata ccaacctgaa
    26461 cggttagccc ggcccggcgg cacataaata aaggtcagat tcggttccga gagatcgggt
    26521 gaaatctgcg ggtttaccag cgtcacttta cccggtaacg cttcaaacgc ggcgtacagt
    26581 ttacgtgtca gaacaccgat atcctgcggg ctggcggaaa cgctaaggtt attgcgacgc
    26641 gcaaagcgga tcagattgcg gtagctctgc atcatcgcgt cgagcaactc gttgtgcgcc
    26701 tcacgcacct gatcaatctt ccagttagcg cgattatcaa gcatcgccag acgagcatcg
    26761 tcccattccc actctttcac caactggctt aacacttcac gacgccagcc tacgcaggcg
    26821 cgttcacggc taagtttttc acacactttt aaatagaagc agcggcgaac taaatccaga
    26881 cgagtaaaat cttcgatcgc cgtcagatat tcagtgacgc gctccagcat catgcaatac
    26941 gggtcgagac caaacgacac tatttcgccg tcgtgcaaac gctgtttgat atctttcgcc
    27001 agcagacgtg ggttcgggta ttcccaggaa taggcttcca gcagcagtgt tttcagcacc
    27061 gctttgtatg gggaatcgat actcttgtag agctgccaaa ggctggcacc aaagtactct
    27121 tcagcagaaa gcgagcttaa gccaccaaga tccagccatt catttggcgt cagcacgccc
    27181 tgcgcgtaaa gcgtcatcac atagtcgtcg taatgctctt cttcgtcgca cggcaccata
    27241 ttccacagaa tacgcttacc ggcgagacgc acggcggtac gataaaattc gtcaagcagc
    27301 agtatatgct gggtggagcc acaatcttcg ccccccaggc tgccgctttc attatgacgg
    27361 aagcggtttt catcaatcag gaagaagctg acttccacac ccagcgaggc ggcccagctt
    27421 tccagcagac tacatttacg ttgtagcaac tggcgctctt cgctatcgag ccaggattga
    27481 tgacagaccc agatatccag gtcagaggaa caactttgcc ctacggacga ggtgctgccc
    27541 atggtgtata caccagtaat tggaagctca cctttcggtg ggtcctgtac tgacattcca
    27601 cgatacagtt caagctcgtt caggtagtgg cgttgagttt catcaggcgt gtaaaggcaa
    27661 atgcctttgg gaacgttacc atcaaggtaa cccggcatta gcggatggtg atagtgcaac
    27721 aatgtcggca gtagactgta gacctgttgg aatgcaggcc ccatagcagc aagcgcgcga
    27781 tccacacgca attgatttat ggcatccagt ctctgtttca gagtctcaat atagaggtac
    27841 aagacgtatc gcctgatttg ctacccgtca tgactgtgat tccgccaaca tcaacggtaa
    27901 cacgcggcat tcgggatatt tcgtatgtca aaggtaaccg ttaccacttt tcgcgcctgg
    27961 tttttttagt ttcacgacga aaaaatggtc taaaacgtga tcaatttaac accttgctga
    28021 ttgaccgtaa agaaagatgc gctacataca agtgtagcac cgtttattct ctgtaaattc
    28081 cttattacga aggctcaaag cgcctgtcag gatccactgc cagacctcat tttacggttt
    28141 gcgcaggcgt ctacgtttca ccacaacact gacatcgctc tggcaaggat gttaggatgg
    28201 accacggatg ataatgacgg taacaagcat gttagacaat gttttaagaa ttgccacacg
    28261 ccaaagccca cttgcactct ggcaggcaca ctatgtcaaa gacaagttga tggcgagcca
    28321 tccgggcctg gtcgttgaac tggtaccgat ggtgacgcgc ggcgatgtga ttcttgatac
    28381 gccgctggcg aaagtaggcg ggaaaggctt atttgtgaaa gagctggaag tcgcgctcct
    28441 ggaaaatcgc gccgatattg ccgttcattc aatgaaagat gtgcccgttg aattcccgca
    28501 aggtctgggg ctggtcacca tttgcgagcg cgaagatccc cgcgatgcct ttgtatccaa
    28561 cacctatgac agtctggatg cgctaccggc aggcagtatc gtcgggacgt ccagtttacg
    28621 tcgccagtgc caactggctg aacgccgccc ggatctgatt atccgctccc tgcgcggcaa
    28681 cgtcggcact cgtctgagca aactggataa cggcgaatat gatgccatca ttcttgccgt
    28741 agccggacta aaacgtttag gtctggagtc ccgcattcgc gccgcgttgc cacccgagat
    28801 ttctcttccg gcggtagggc aaggtgcggt gggcattgaa tgccgccttg atgatgcacg
    28861 cactcgcgag ctgctcgccg cgctgaatca ccacgaaact gcactgcgcg ttaccgccga
    28921 acgcgccatg aatacccgtc tcgaaggcgg atgtcaggtg ccaattggta gctacgccga
    28981 gcttattgat ggcgaaatct ggctgcgtgc gttggtcggc gcgccggacg gttcgcagat
    29041 tattcgcggt gaacgccgcg gtgcgccgca agatgccgaa caaatgggga ttgcgctggc
    29101 agaagagcta ctgaataacg gcgcgcgcga gatcctcgct gaagtctata acggagacgc
    29161 cccggcatga gtatcctggt cacccgccca tctcccgcag gagaagagtt agtgagccgt
    29221 ctgcgcacac tggggcaggt ggcctggcat tttccgctga ttgagttttc tccgggtcga
    29281 caattaccgc aacttgctga tcaactggca gcgctgggag agagcgatct gttgtttgcc
    29341 ctctcgcaac acgcggttgc ttttgcccaa tcacagctgc atcagcaaga tcgtaaatgg
    29401 ccccgactac ctacttattt cgccattgga cgcaccaccg cactggcact acataccgta
    29461 agcggacaga agattctcta cccgcaggat cgggaaatca gcgaagtctt gctacaatta
    29521 cctgaattac aaaatattgc gggcaaacgt gcgctgatat tacgtggcaa tggcggtcgt
    29581 gagctaattg gggataccct gacggcacgc ggtgctgagg tcactttttg tgaatgttat
    29641 caacgatgcg caatccatta cgatggtgca gaagaagcga tgcgctggca atcccgcgag
    29701 gtgacgacgg tcgttgttac cagcggtgaa atgttgcagc aactctggtc gctgatccca
    29761 caatggtatc gtgagcactg gttactacgc tgtcgactat tggtcgtcag tgagcgtttg
    29821 gcgaaactcg cccgggaact gggctggcaa gacattaagg tcgccgataa cgctgacaac
    29881 gatgcgcttt tacgggcatt acaataactc tcataacagg aagccataat gacggaacaa
    29941 gaaaaaacct ccgccgtggt tgaagagacc agggaggccg tgggcaccac gtcacaacct
    30001 gtcgcaacag aaaaaaagag taagaacaat accgcattga ttctcagcgc ggtggctatc
    30061 gctattgctc tggcggcggg cgtcggtttg tatggctggg gtaaacaaca ggccgtcaat
    30121 cagactgcca ccagcgatgc cctggctaac caacttacgg cactgcaaaa agcccaggag
    30181 agccaaaaag ccgagctgga aggcattatc aagcaacaag ctgtacagct tgagcaggcg
    30241 aatcgtcagc aagaaacgct ggcaaaacag ctggatgaag tccaacaaaa ggtcgccacc
    30301 atttccggca gcgatgctaa aacctggctg ctggcccagg ccgattttct ggtgaaactc
    30361 gccggacgga agctgtggag cgatcaggac gtcacgaccg ctgcagcgtt gctgaaaagt
    30421 gcagacgcca gcctggcgga tatgaatgac ccgagtctga ttaccgttcg ccgggcaatt
    30481 accgatgata tcgccagcct ttctgcggta tcgcaggtgg attatgacgg tatcatcctt
    30541 aagcttaatc agctttcaaa tcaggtagat aatctgcgtc tggccgataa tgacagcgat
    30601 ggttcgccga tggattccga cggtgaagag ctttccagtt ccatcagcga atggcgtatc
    30661 aatctgcaaa aaagctggca gaactttatg gacaacttca ttacgattcg ccgtcgtgat
    30721 gacaccgccg taccgctgtt agcgccaaat caggatatct atctgcgcga aaatattcgc
    30781 tctcgcctgc tggtcgcagc acaagctgta ccgcgtcacc aggaagagac ttatcgccag
    30841 gcgctggaga acgtctccac ctgggtacgt gcttactacg atactgatga tgccaccacc
    30901 aaagcgttcc tcgacgaggt ggaccagtta agccagcaaa atatctcgat ggatcttccg
    30961 gaaaccctgc aaagccaggc gatgctggaa aaattgatgc agacccgcgt gcgtaacctg
    31021 ctggcacaac cggcagcagg gacaacggaa gctaaacctg cacctgcacc gcaagctgat
    31081 gctccggcag ccgcgccgca aggagaataa ccatgctaaa agtgttattg ctctttgtgt
    31141 tgctgattgc ggggatcgtg gttggcccga tgattgccgg ccatcagggt tacgtgctga
    31201 tccagaccga caactacaat attgaaacca gcgtcacggg cctcgcgatt attttgattc
    31261 tggcgatggt agtgctgttt gccattgagt ggctactgcg gcgaatcttc cgcacaggcg
    31321 cgcacacccg tgggtggttt gtcggacgta agcgtcgccg tgcacgtaag cagaccgaac
    31381 aggcgctgct gaaactggcg gaaggcgatt atcagcaagt tgaaaagctg atggcgaaaa
    31441 atgccgatca cgcggaacaa ccggtggtga actatctact ggctgccgaa gccgcgcaac
    31501 aacgtggtga tgaagcacgc gccaaccaac atctggaacg cgcagcggag ctggccggca
    31561 atgacaccat tccggtagaa atcacccgtg tacgtctgca actggcccgt aatgaaaacc
    31621 atgcagcacg tcacggcgtg gataagctgc tggaagttac gccacgccat ccggaagtat
    31681 tacgtctggc ggaacaggcg tatatccgca ctggtgcatg gagttcgctg ctggatatca
    31741 tcccatcaat ggcgaaagcc catgttggtg atgaagaaca tcgtgcaatg ctggaacaac
    31801 aggcatggat tggcctgatg gatcaggcgc gtgccgataa cggtagtgaa ggtttgcgta
    31861 actggtggaa aaaccaaagc cggaaaacgc gtcatcaggt ggccttgcag gtggcaatgg
    31921 cggaacatct tattgagtgt gacgatcatg atactgccca gcaaattatc atcgatggcc
    31981 tgaaacgcca gtacgacgat cgcctactgc tgccgattcc tcgactgaaa accaataacc
    32041 cggaacagct ggaaaaagtg ctgcgccaac agatcaaaaa cgtgggcgat cgcccgctgt
    32101 tgtggagcac actgggtcag tcgctgatga aacacggaga atggcaggaa gcatcgctcg
    32161 ccttccgcgc agcgctgaaa caacgtccgg acgcctacga ttacgcatgg cttgccgacg
    32221 cgctggacag actgcacaag ccggaagaag ccgcagcgat gcgtcgcgac ggtttgatgt
    32281 taacgttgca gaacaacccg ccacagtagc tctttctcac ccggaggcaa gcacctccgg
    32341 ggcctttctg atatataaaa aaacgcctgc tcttattacg gagcaggcgt taaaacaggt
    32401 ctgtatgaca acaagtgggt gcttcactca acgttgtgtc catggtgtct gatgaggcat
    32461 aagcgacatc tgtcagtgga cgataagcac cgtaaacggc tctgcgtcat tccggagttt
    32521 atgaggcact aaggcgaaca taagagatgg aatgagcatc tactcgttta ttatgccaca
    32581 gagaaccggg aaataacatc ccttaacact tgttatgaga taattctgta atcctctttg
    32641 cttcctgagt aataacttcc tgagtgaata
//
LOCUS       DADSZD010000034        32059 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.034, whole genome shotgun sequence.
ACCESSION   DADSZD010000034 DADSZD010000000
VERSION     DADSZD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.034"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="J1Z28_001466"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="J1Z28_001466"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBA7716311.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFSEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="J1Z28_001467"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="J1Z28_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBA7716312.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="J1Z28_001468"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="J1Z28_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBA7716313.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="J1Z28_001469"
     CDS             3807..3995
                     /locus_tag="J1Z28_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716314.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="J1Z28_001470"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="J1Z28_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBA7716315.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICTGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..7374
                     /gene="bcsA"
                     /locus_tag="J1Z28_001471"
     CDS             4756..7374
                     /gene="bcsA"
                     /locus_tag="J1Z28_001471"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBA7716316.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMSNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            7385..9724
                     /gene="bcsB"
                     /locus_tag="J1Z28_001472"
     CDS             7385..9724
                     /gene="bcsB"
                     /locus_tag="J1Z28_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBA7716317.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9731..10837
                     /gene="bcsZ"
                     /locus_tag="J1Z28_001473"
     CDS             9731..10837
                     /gene="bcsZ"
                     /locus_tag="J1Z28_001473"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBA7716318.1"
                     /translation="MNVLRSGLVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNLLDWTQNNLAQGSLKEHLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10819..14292
                     /gene="bcsC"
                     /locus_tag="J1Z28_001474"
     CDS             10819..14292
                     /gene="bcsC"
                     /locus_tag="J1Z28_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HBA7716319.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTSDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRGQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPAIDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEALAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDTADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            14374..16362
                     /gene="hmsP"
                     /locus_tag="J1Z28_001475"
     CDS             14374..16362
                     /gene="hmsP"
                     /locus_tag="J1Z28_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBA7716320.1"
                     /translation="MRVSRSLTIKQMALVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQQRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            16545..17831
                     /locus_tag="J1Z28_001476"
     CDS             16545..17831
                     /locus_tag="J1Z28_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBA7716321.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            18051..19547
                     /locus_tag="J1Z28_001477"
     CDS             18051..19547
                     /locus_tag="J1Z28_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBA7716322.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(19643..20572)
                     /locus_tag="J1Z28_001478"
     CDS             complement(19643..20572)
                     /locus_tag="J1Z28_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7716323.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSSAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            20804..21571
                     /gene="pdeH"
                     /locus_tag="J1Z28_001479"
     CDS             20804..21571
                     /gene="pdeH"
                     /locus_tag="J1Z28_001479"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBA7716324.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            21641..23701
                     /locus_tag="J1Z28_001480"
     CDS             21641..23701
                     /locus_tag="J1Z28_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBA7716325.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWL
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(23742..25064)
                     /locus_tag="J1Z28_001481"
     CDS             complement(23742..25064)
                     /locus_tag="J1Z28_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA7716326.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFAVMVPLAGLLADAFGRRKSMVVITTLIILFALF
                     AFNPLLGSGNPALVFVFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(25457..26470)
                     /gene="yhjD"
                     /locus_tag="J1Z28_001482"
     CDS             complement(25457..26470)
                     /gene="yhjD"
                     /locus_tag="J1Z28_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBA7716327.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(26519..27490)
                     /locus_tag="J1Z28_001483"
     CDS             complement(26519..27490)
                     /locus_tag="J1Z28_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716328.1"
                     /translation="MLEKTINNAICALLFRCEQQSVKEMDKIHAMQLFIKVAELESFS
                     RAADFFALPKGSVSRQIQALEHQLGTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSE
                     LDGLFQQDATSISGKLRIDIPSGIAKSLLLPRLSEFLYLHPGIELELSSHDRPVDILH
                     DGFDCVIRTGALPEDGVIARSLGKLTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTP
                     HLGVHPLGFEVASVNGVQWFKSGGMLTVNSSENYLAAGLAGLGIIQIPRIAVREALRA
                     GRLIEVLPGYRAEPLSLSLVYPQRRELSRRVNLFMQWLAGVMKEHLD"
     gene            27938..28540
                     /locus_tag="J1Z28_001484"
     CDS             27938..28540
                     /locus_tag="J1Z28_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA7716329.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(28591..30240)
                     /gene="treF"
                     /locus_tag="J1Z28_001485"
     CDS             complement(28591..30240)
                     /gene="treF"
                     /locus_tag="J1Z28_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HBA7716330.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            30645..32042
                     /gene="ccp"
                     /locus_tag="J1Z28_001486"
     CDS             30645..32042
                     /gene="ccp"
                     /locus_tag="J1Z28_001486"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBA7716331.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNARGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         7697 a         8051 c         8763 g         7548 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag gggtgaaact ctgcggcctt tttcgttctt attgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggaga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcgatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag accagatacc
      421 tgcattctgt cgcccttcag cgcgccgcca tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttttgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacg cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccaa
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcgctc tgcatgccgc cattttcgcg aacttccttc
      781 aaaaaaccac cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gacaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga catcaatccg
     1021 gcggcttcaa tatccgacca ggaaagcgaa cagatattaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag atcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggtttgct gttgttggcg gtgcagtttg tgcgggtatg
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgaccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagaatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac gaataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caagatcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg cattagcaga
     1621 aacgcggcaa acaccagatt gaggagcgga tggaagttaa gatatcccgc ccacaacaga
     1681 ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca ttggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgc taaaggcagc ggcatgcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 tcggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacagcggga gagtggcgca ttccacggga taaccatatt
     2581 tgcaccgcag gccaataata aacgttcatc ggtggcgcgc aggctggcgg tattttcccg
     2641 tacgaggatt ttcatcgcac taccgcgctg gcgacgcaga gtatgaatgc tgcgggccag
     2701 tggctcgatt tgtgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 agcaccctcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ttccgcctcc tggtgttgaa ctaatgtcca gcaaccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaagca atatccagca aatgttggtc
     3001 gccctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gtaacaatga
     3061 aaattgttta tcgttattat ttccggaatt aattaccaaa agcgaacaat ggtttagccg
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tgttttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata atgttatttt
     3301 tcccggccct tgagaatcat ctaattgaaa gattttcgct ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcaac
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagcaggtc acaagacatg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag cacattcccg
     3721 tctcgccgta aacaataaac cgacgaaatg ctcacagtta gaattgtctc atgaacggta
     3781 cggttatttc atagggatca agaaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttttggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gttcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aacgttgatt ttacccaccg tcagggttgg gccagagcta tgctggatgg
     4201 ccaggactgg cgtgacgccg ggttgcgcta tacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgcaccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taattgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 tgcacatatt ttgattaatg acttccgtat tggcagccag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattgct gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgt ctggcagcta agcaaccagt cggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaat tattccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgca
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggcg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatccgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tcttattcaa acatgctggt tactgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg caggcgggca ttttcaggtc tacaaaatat tcgtggacgt
     5101 taccatcaat ggatgaacga gctgcctgag cgcgttagcc ataaaacaca gcatcttgat
     5161 gagaaaaaag agctcggtca tttgagtgcc ggggcgcggc ggttgatcct tggtatcatc
     5221 gtcaccttct cgctgattct ggcgttaatc tgcgttactc agccgtttaa cccgctagcg
     5281 cagtttatct tcctgatgct gctgtggggg gtagcgctga tcgtacggcg gatgccgggg
     5341 cgcttctcgg cgctgatgct gattgtgctg tcgctgaccg tttcttgccg ttatatctgg
     5401 tggcgttaca cctcgacgtt aaactgggac gatccggtca gcctggtgtg cgggcttatt
     5461 ctgctgttcg ccgaaacgta cgcgtggatt gtgctggtgc tcggctattt ccaggtcgta
     5521 tggcctctga atcgtcagcc ggtgccattg ccgaaagata tgtcgctgtg gccgtcggtg
     5581 gatatctttg tcccgactta caacgaagat ctcaacgtgg tgaaaaatac catttacgct
     5641 tcgctgggta tcgactggcc gaaagacaag ctgaacatct ggatcctcga tgacggcggc
     5701 agggaagagt ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc ccgtaccact
     5761 catgaacatg cgaaagcggg caacatcaac aatgcgctga aatatgccaa aggcgagttc
     5821 gtgtcgatat tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat gaccatgggc
     5881 tggttcctga aagagaaaca gctggcgatg atgcagacac cgcaccactt cttctcgccg
     5941 gatccgtttg aacgcaacct ggggcgtttc cgtaaaacac cgaacgaagg cacgctgttc
     6001 tatggtctgg tgcaggacgg caatgatatg tgggatgcca ctttcttctg cggctcatgt
     6061 gcggtgattc gccgtaagcc gctggatgag attggcggta ttgctgtcga aacggtaacc
     6121 gaagatgcgc acacttctct gcgtctgcac cgtcgtggct acacctccgc gtatatgcgt
     6181 attccgcagg cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg tcagcgtatt
     6241 cgctgggcgc gcgggatggt gcaaatcttc cgtctcgata acccgctcac cggtaaaggg
     6301 ctgaagtttg ctcagcggct atgttacgtc aacgccatgt tccacttctt gtcgggcatt
     6361 ccacggctga tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc ctacatcatc
     6421 tatgcgccag cgttgatgat cgccctgttc gtgctgccgc atatgattca tgccagcctg
     6481 accaactcga agatccaggg caaatatcgc cactctttct ggagtgaaat ctacgaaacg
     6541 gtgctggcgt ggtatatcgc accaccgacg ctggtggcgc tgattaaccc gcacaaaggc
     6601 aaatttaacg tcaccgccaa aggtggactg gtggaagaag agtacgtcga ctgggtgatc
     6661 tcgcggccct acatcttcct tgttctgctc aacctggtgg gcgtagcggt tgggatctgg
     6721 cgctacttct atggtccgcc aaccgagatg ctcaccgtgg tcgtcagtat ggtgtgggta
     6781 ttctacaacc tgattattct cggcggcgca gtcgcggtat cggtagaaag caaacaggta
     6841 cgccgatcgc accgcgtgga gatgacgatg cccgcagcaa ttgcccgcga agatggtcac
     6901 ctgttctcgt gtaccgttca ggatttctcc gacggtggtt tggggatcaa gatcaacggt
     6961 caggcgcaga ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg tcagcaggaa
     7021 tacgtcttcc cggctcaggt ggcgcgcgta atgagtaatg aagttgggct gaaattaatg
     7081 ccgctcacta cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg tgcggataca
     7141 tgggcgctct ggcaggacag ctacccggaa gataagccgc tggaaagtct gctggatatt
     7201 ctgaagctcg gcttccgtgg ctaccgccat ctggcggaat ttgcgccttc ttcggtgaag
     7261 ggcatattcc gtgtgctgac ttctctggtt tcctgggttg tatcgtttat tccgcgccgc
     7321 ccggagcgga gcgaaacggc acaaccatcg gatcaggctt tggctcaaca atgatgataa
     7381 cgcgatgaaa agaaaaatat tctggatttg tgcagtggct ctggggatga gtgcgttccc
     7441 ctctttcatg acgcaggcga cgccagcaac gcaaccactg atcaatgctg agccagctgt
     7501 agccgcccag gcggagcaaa atccgcaggt ggggcaagtg atgccgggcg tgcagggtgc
     7561 agatgcgcca gtcgtggcgc agaacggtcc ttcccgtgat gtgaagctga cctttgcgca
     7621 aattgcgccg ccgccgggca gcatggtgct acgtggcatt aacccgaacg gcagcattga
     7681 gtttggtatg cgcagcgatg aagtggtgac gaaggcgatg ctcaacctcg aatacacgcc
     7741 atcgccatca ttactgcctg tccagtcgca gttaaaggtt tatctcaatg atgaactgat
     7801 gggcgtgctg ccagtgacca aagaacagtt gggtaaaaaa acgctggcac aaatgcccat
     7861 taacccgctg tttattactg atttcaaccg tgtgcggctg gagtttgtcg gccattatca
     7921 ggacgtgtgc gaaaacccgg ccagcaccac gctttggctg gatgttgggc ggagcagtgg
     7981 actggatctg acctatcaga ccctgaatgt gaagaatgac ctgtcacact tcccggtgcc
     8041 attctttgac ccgcgtgata accgcaccaa caccttgccg atggtctttg cgggtgcgcc
     8101 ggatgttgag ctgcaacaag catctgccat tgtcgcctcg tggtttggtt cgcgttctgg
     8161 ctggcgtggg cagaacttcc cggtgctcta taaccaactg ccggatcgca atgccattgt
     8221 ctttgccacc aatgacaaac gcccggactt cctgcgcgat catccggcgg taaaagcccc
     8281 ggtgattgag atgattaacc atccgcagaa tccttacgtc aaactgctgg tggtgtttgg
     8341 ccgtgacgac aaagacttgt tgcaggcagc gaaaggtatc gctcagggta acattctgtt
     8401 ccgtggtgaa agtgtggtag tgaatgaagt gaaaccgctg ctaccgcgta aaccgtacga
     8461 tgcgccgaac tgggtgcgta ccgatcgtcc ggtcaccttt ggtgaactga aaacctatga
     8521 agaacaatta caatccagcg gtcttgagcc agcagcgatt aacgtttcgc taaacctgcc
     8581 gccggatctc tacctgatgc gcagtaccgg cattgatatg gatattaatt accgctacac
     8641 catgccgccg gtgaaagaca gttcgcggat ggatatcagc ctgaataacc agttcctgca
     8701 atccttcaac ctgagcagca aacaggaggc gaaccgcctg ctgctgcgga ttccggtatt
     8761 acaaggtttg ctggatggca aaacggatgt ctctattccg gcgctgaaac tgggcgcgac
     8821 caaccagttg cggttcgact ttgagtatat gaacccgatg ccgggcggtt cggtggataa
     8881 ctgtattacc ttccagccgg tgcagaatca tgtggtgatt ggtgacgact ccaccatcga
     8941 cttctcgaag tattaccact tcatcccgat gccggatcta cgcgcctttg ctaacgcggg
     9001 cttcccgttc agccggatgg cggatttgtc gcaaaccatc accgtgatgc cgaaaacgcc
     9061 aaacgaagca cagatggaaa ccttgctgaa taccgttggc tttatcggtg cgcagacggg
     9121 cttcccggcg attaacctga cggtgaccga tgatggcagc accattcagg gcaaagatgc
     9181 cgacatcatg atcgtcggtg gtatcccgga caaactgaaa gacgataagc agatcgacct
     9241 gttggtgcag gctaccgaaa gctgggtgaa aacgccgatg cgccagaccc cgttccccgg
     9301 tattgtgccg gacgagagcg atcgcgcggc agaaactcag tcaacgctga cctcttccgg
     9361 tgcgatggcg gcggtgattg gcttccagtc gccgtataac gaccagcgca gcgtgattgc
     9421 gctgctggca gatagcccac gcggttatga aatgcttaac gatgcggtga acgatagcgg
     9481 caaacgcgcc accatgttcg gttcggtcgc ggtgatccgc gagtccggta tcaacagcct
     9541 acgtgttggc gatgtttatt acgtaggtca tctgccgtgg ttcgagcgct tgtggtatgc
     9601 gctggcaaac catccgattc tgctggcggt actggcggct atcagtgtga tattgctggc
     9661 gtgggtactg tggcgtctgc tgcgtattat tagtcgtcgt cgtcttaacc cggataacga
     9721 gtaattgaag atgaatgtgt tgcgtagtgg actcgtgacg atgctgctgt tggctgcctt
     9781 tagtgttcag gcagcctgta cctggcctgc ctgggagcag tttaaaaagg attacatcag
     9841 tcaggaaggg cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct ccgaagggca
     9901 aagttacggc atgttctttg ccctggcggc taacgaccgt gcagctttcg ataatcttct
     9961 cgactggacg cagaacaatc tcgctcaggg ttctttaaaa gaacatttgc ccgcctggct
    10021 gtggggcaag aaagagaaca gtaagtggga agtgctggac agcaattcgg cctccgatgg
    10081 tgatgtctgg atggcctggt cgttgctgga ggcggggcgt ttgtggaaag agcagcgtta
    10141 taccgacatc ggcagcgcat tgctaaaacg tatcgcgcgg gaggaagtgg tgacggtgcc
    10201 tgggctgggc tccatgttgt taccgggcaa agtgggtttt gctgaggata acagctggcg
    10261 ttttaacccc agctacctgc cgccgacgct ggcgcagtat ttcacccgct ttggcgcgcc
    10321 gtggaccacg ctgcgcgaaa ccaatcaacg tttattgctg gaaaccgccc cgaaaggctt
    10381 ttcgccagac tgggtgcgct atgagaaaga caaaggctgg cagctaaaag ccgaaaaaac
    10441 attgatcagc agctacgacg ctatccgcgt ttacatgtgg gtaggcatga tgcctgacag
    10501 cgatccgcaa aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat tcactgagaa
    10561 aaacggttat ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg gtaaaggacc
    10621 ggtcggtttt tctgccgcca tgctgccctt tttacaaaac cgcgatgcgc aggccgttca
    10681 gcgccagcgc gtggccgata actttcctgg cagcgatgcc tattacaact atgtgctgac
    10741 cctgtttgga caaggctggg atcaacaccg tttccgcttc tcgacaaaag gtgagttatt
    10801 acctgactgg ggccaggaat gcgcaaattc acactaaaca tattcacgct ttccctcggt
    10861 ctggccgtca tgccgatggt cgaggcagca ccaaccgctc agcaacagtt gctggagcaa
    10921 gttcggttag gcgaagcgac ccatcgtgaa gacctggtgc aacagtcgtt gtatcggctg
    10981 gaacttattg atccgaataa cccggatgtg attgctgccc gttttcgttc tctgttacgt
    11041 cagggcgata ttgatggcgc acaaaaacag ctcgatcggc tgtcgcagtt agcgccgagt
    11101 tcaaatgcgt ataaatcgtc acggactacg atgctgcttt ccacgtcgga tggtcgtcag
    11161 gcactgcaac aggcaagatt gcaggcgacg actggtcatg cggaagaagc tgtggcgagt
    11221 tacaacaaac tgttcaacgg tgcgccgccg gaaggtgaca ttgctgtcga gtactggagt
    11281 acggtggcga aaattccggc ccgacgtggc gaagcgatta atcagctaaa acgcattaat
    11341 gcggatacgc cgggcaacac gggcctgcaa aacaatctgg cgctattact gtttagtagc
    11401 gatcgccgtg atgaaggttt tgccgtcctg gaacagatgg cgaaatcgaa cgccgggcgc
    11461 gaaggggcct ctaaaatctg gtacgggcag attaaagaca tgcctgtcag cgatgccagc
    11521 gttcaggcac tgaaaaaata tctctcgatc tttagcgatg gcgatagcgt agctgctgca
    11581 caatcgcaac tggcagaaca gcaaaaacag ctggccgacc ctgctttccg cgctcgtgcg
    11641 caaggtttag cggcggtgga ctctggcatg gcgggtaaag ccattcccga actacaacag
    11701 gcggtgcggg cgaacccgaa agacagtgag gctctggggg cgctgggcca ggcgtattct
    11761 cagaaaggcg atcgcgctaa tgcggtggcg aatctggaaa aagccctcgc gctggatccg
    11821 cacaacagca ataacgacaa atggaacagt ctgctgaaag taaaccgcta ctggctggcg
    11881 atccagcagg gcgatgccgc gctgaaagcc aataatcctg accgggcaga acggctgttc
    11941 cagcaggcac gtaatgtcga taacaccgac agctatgcag tgcttgggct gggcgatgtg
    12001 gcgatggcgc gcaaagatta tcccgccgcc gaacgctatt accagcagac cttgcgtatg
    12061 gacagcggca acactaacgc cgtgcgcggg ctggcgaata tttaccgcca gcagtcgcca
    12121 gaaaaagccg aagcgtatat cgcctcgctc tctgccagcc agcggcgcag cattgatgat
    12181 atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg cagaggcact ggaaaaccag
    12241 gggaaatggg cgcaggcggc agcacttcag cggcaacgac tggcgctgga ccccggcagt
    12301 gtatggatta cttaccgact ttcgcaggat ctctggcaag ccggacaacg cagccaggcc
    12361 gatacgttaa tgcgcaatct ggcgcagcag aagccgaacg acccggagca ggtttacgct
    12421 tacgggctgt acctctccgg tcacgaccag gacagagcgg cgctggcgca tatcaacagc
    12481 ctgccgcgcg ggcagtggaa cagcaatatt caggagctgg ttaatcgact gcaaaacgat
    12541 caggtgctgg aaaccgctaa tcgcctgcga gaaaacggta aagaggcaga agcggaagcg
    12601 atgctgcgcc agcaaccgcc ttcctcgcgt attgacctca cgctggctga ctgggcgcag
    12661 cagcgacgtg attacaccgc cgcccgcgct gcatatcaga atgtcctgac gcgggagcca
    12721 actaacgccg atgccattct tggtctgacg gaagtggata ttgctgccgg tgacaaagcg
    12781 gcggcacgta gccagctggc gaaactgcct gccattgata acgcctcgct gaacacacag
    12841 cggcgcgtgg cgctggcaca ggcgcagctt ggcgataccg cagcggcgca gcagacattt
    12901 aataagttga tcccgcaggc aaaatctcag ccaccgtcga tggaaagcgc gatggtactg
    12961 cgtgatggtg cgaagtttga agcgctggcg ggcgatccaa cgcaggcgct ggaaacctac
    13021 aaagacgcta tggttgcatc cggtgtgacc acgacgcgtc cgcaggataa cgacaccttt
    13081 acccggctga cccgtaacga cgagaaagat gactggctga aacgcggcgt gcgcagcgat
    13141 acggcggacc tctatcgcca gcaggatctt aacgtcactc tcgagcacga ttactggggt
    13201 tcgagcggta ccggtggtta ctccgatctg aaagcgcaca ccactatgtt gcaggtggac
    13261 gcgccctatt ctgacgggcg gatgttcttt cgcagtgatt tcgtcaatat gaacgtcggc
    13321 agtttctcca ctaatgccga tggcaaatgg gatgacaact ggggcacctg tacattacag
    13381 gactgtagcg gcaaccgcag ccagtcggat tccggtgcca gcgtggcggt cggctggcga
    13441 aatgacgtct ggagctggga tatcggtacc acgccgatgg gcttcaacgt ggtggatgtg
    13501 gtcggcggca tcagttacag cgatgatatc gggccgctgg gttacaccgt taacgcccac
    13561 cgtcggccta tctccagttc tttgctggcc tttggtgggc aaaaagactc tccgagcaat
    13621 accgggaaaa aatggggcgg cgtgcgtgcc gacggcgtgg ggctaagtct gagctacgat
    13681 aaaggtgaag caaacggcgt ctgggcatcg cttagcggtg atcagttaac cggtaaaaat
    13741 gtcgaagata actggcgtgt gcgctggatg acgggctatt actataaggt catcaaccag
    13801 aacaatcgcc gcgtcacaat cggcctgaac aacatgatct ggcattatga caaagacctg
    13861 agtggctact cactcggtca gggcggttac tacagtccgc aggaatacct gtcgtttgcc
    13921 ataccggtga tgtggcggga gcgcacggaa aactggtcgt gggagctggg ggcgtctggc
    13981 tcgtggtcgc attcacgcac caaaaccatg ccgcgttatc cgctgatgaa tctgatcccg
    14041 accgactggc aggaagaagc tgcgcggcaa tccaacgatg gcggcagcag tcagggcttt
    14101 ggctacacgg cgcgggcatt acttgaacga cgtgttactt ccaactggtt tgtcggcacg
    14161 gcaattgata tccagcaggc aaaagattac gcccccagcc atttcctgct ctacgtacgt
    14221 tattccgccg ccggatggca gggtgacatg gatttaccgc cgcagccgct gataccttac
    14281 gccgactggt aagttttcag atagcgcctc tcttaatgcc gctgcgatcg ggtatactcg
    14341 ggcggcaatc tgggatttcc ggggggagac aatttgcgcg taagtcgctc gttaacaatc
    14401 aagcagatgg cactggtggc agccgttgtc ctggtgttcg tttttatttt ttgcaccgtt
    14461 ttgctgttcc atctggtcca gcagaatcgc tataacacgg ctacgcaact ggaaagcatt
    14521 gctcgctctg tccgcgaacc cttatcttct gccattttga aaggcgatat tcccgaagcg
    14581 gaagctattc ttgccagcat taaaccggca ggcgtggtca gccgtgccga tgtagtgctg
    14641 cctaaccagt tccaggcgct gcgtaaaagt tttattccag agcgtccggt gccggtaatg
    14701 gttactcgcc tgtttgagct accggtgcaa atctcgctgg gcgtttactc gctggaacgt
    14761 ccggcaaacc cgcagccaat tgcctatctg gtgttacagg cggattcctt ccgcatgtat
    14821 aagttcgtga tgagcaccct ctcaacgtta gtgaccattt acttactttt gtcgttaata
    14881 ttgacggtgg cgattagctg gtgcattaac cgcctgattt tgcatccatt acgcaatatt
    14941 gctcgcgaac ttaacgccat cccaccccag gagcttgttg gtcaccaact ggcattaccg
    15001 cgtctgcatc aggacgatga aatcggtatg ttggtgcgca gttacaacct caaccagcaa
    15061 ttgctgcagc gccattatga agaacagaac gaaaatgcga tgcgcttccc ggtgtcggat
    15121 ttgccgaaca aagccttgct gatggagatg ctggagcagg tggtggcgcg taaacaaacc
    15181 accgcgctga tgatcatcac ctgtgaaacc ctgcgtgata ctgcgggtgt gctgaaagag
    15241 gcgcaacgag aaattctgct gctgacgctg gtggaaaaac tcaaatcggt actgtcgcca
    15301 cgcatgatcc tcgcgcagat tagcggttat gactttgctg tcattgccaa cggtgtacag
    15361 gaaccgtggc acgctatcac attaggtcag caagtgctca ctatcatgag cgagcgcctg
    15421 ccgattgaac gtattcaact ccgtccgcac tgtagcattg gcgtggcgat gttctacggc
    15481 gatctcaccg ccgaacagct ttacagtcgc gctatttctg cggcatttac cgctcgccat
    15541 aaaggcaaaa atcagattca gttctttgat ccgcagcaga tggaagccgc ccagcagcgg
    15601 ttgacggaag agagcgatat ccttaatgca ctggaaaatc atcagtttgc gatttggtta
    15661 cagccacagg tcgagatgac cagcggtaaa ctggtcagtg cggaagtgtt actgcgtatc
    15721 cagcaaccgg atggcagttg ggacctgccc gatggcttaa tcgatcgcat tgagtgctgt
    15781 gggctgatgg ttaccgtcgg tcactgggtg ctggaagagt cctgtcgatt gcttgctgcc
    15841 tggcaagagc gcggcattat gctgcccttg tcggtaaacc tctcagcgct gcaactgatg
    15901 catccaaata tggtggcgga tatgctggaa ttgttaaccc gctatcgtat tcagccggga
    15961 acactgattc tggaagtgac agaaagccga cgtattgacg atcctcatgc tgcggtggca
    16021 atcctccgtc cgctgcgcaa tgccggagtt cgggtggcgc tggatgattt cggcatgggc
    16081 tacgcagggc tgcgtcagct gcagcatatg aaatcgttgc caatcgacgt actgaaaatc
    16141 gacaaaatgt ttgttgaagg tttgccggaa gatagcagca tgattgctgc aattatcatg
    16201 ctggcgcaga gcctgaacct acaaatgatt gccgaaggcg tggagactga agcacaacgc
    16261 gactggctgg caaaagcggg cgttggtatt gcccagggct tcctttttgc tcgcccactc
    16321 cctattgaaa tcttcgaaga gagttacctg gaagaaaagt agctacccca aactgattac
    16381 aaaactttaa aaagtgctgg tttgtgcgag ccagctcaaa ctttttaacc tttttgtttc
    16441 aattatgatc caggtacatt tctgtgatgt tgtctgggtg ttattttaag gccgcaggta
    16501 ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa acctctctgt
    16561 ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc cttggccatt
    16621 tctaccctga aataggcgag caaatgaaac cgcttggcga cgggttcgtt aagctcatta
    16681 agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg ggcatggaaa
    16741 gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa attgtcagta
    16801 ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt gccggaatga
    16861 acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat caggcgaaag
    16921 accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt ggcgcatttg
    16981 ccagcggtaa cattctgcag gtactgctgt ttgccgtact gtttggtttt gcgctccacc
    17041 gtctgggcag caaaggccaa ctgattttta acgtgatcga aagtttctcg caggtcattt
    17101 tcggcatcat caatatgatc atgcgtctgg cacctattgg tgcattcggg gcaatggcgt
    17161 ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg attatctgtt
    17221 tctacatcac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct aaagcgactg
    17281 gtttcagtat cttcaaattt atccgttaca tccgtgaaga actgctgatt gtactgggga
    17341 cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa ctcggctgcc
    17401 gcaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt gatggcacat
    17461 cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt cagatggata
    17521 tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg gcggcagggg
    17581 taacgggtag tggttttatc gtgctggcgg cgacgctctc tgcggtgggc catttgcctg
    17641 tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct cgtgcgctga
    17701 ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg aaagaactgg
    17761 accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa acgcacgaat
    17821 tatcctctta atctcaccac ttatgcccgc actcccttcc ccgagtgcgg gtgtttgcgc
    17881 ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat ttcacttctt
    17941 ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc atttagactg
    18001 accgacaacg ggcagttttt tattaccagg atagttgatc aggggttcac atgcagggca
    18061 caaaaattcg acttttagcg ggcggtttgc tgatgatggc cactgctggc tatgtgcagg
    18121 cagatgcgct ccagcctgat ccagcatggc aacaggggac gctttccaac ggtttacagt
    18181 ggcaagtgct gactacgccc cagcgtccca gcgatcgtgt tgaaattcgc ctgctggtta
    18241 ataccggttc gctcgccgaa agtacacaac agagcggtta cagtcacgcc atccctcgta
    18301 ttgcgctaac gcaaagcggt ggccttgacg cagcgcaggc gcgttcattg tggcagcagg
    18361 ggatcgaccc taaacgcccg atgccgccgg taattgtctc ttatgacacc acgctgttta
    18421 atctgagttt gcccaataac cgtaacgact tgctgaaaga ggcgctctct tatctggcaa
    18481 atgccactgg caaactaact atcacgccag aaaccatcaa ccatgcgttg caaagtcagg
    18541 acatggtggc aacctggcct gccgatacta aagagggctg gtggcgttat cgtctgaaag
    18601 gatcaacctt gttaggtcac gatcctgccg atccgctgaa acaacccgtt gaagcggaaa
    18661 agattaaaga tttctatcag aaatggtaca ccccggatgc aatgacgctg ctggtggtgg
    18721 gaaacgtgga tgcgcgctcg gtcgtcgacc aaatcaataa aacgtttggc gaactgaaag
    18781 gcaaacgtga aacaccggct ccggtgccga cgctttctcc gctgcgtgcg gaagcggtga
    18841 gtattatgac cgacgcggtg cgtcaggacc ggctatctat catgtgggat acgccgtggc
    18901 agccgattcg tgaatcagcc gcactgctgc gctactggcg tgcggacctg gcccgtgagg
    18961 cgctgttctg gcatgttcag caagcgttaa gcgccagtaa cagtaaagac atcggtcttg
    19021 gatttgactg tcgtgtgctg tatctgcgtg cgcagtgtgc catcaacatc gaatcaccaa
    19081 acgacaagct gaacagcaac cttaatctgg tggcgcgtga actggcgaag gttcgcgata
    19141 aaggtctgcc ggaagaagag ttcaatgcgt tagtggcgca aaagaaactg gagctgcaga
    19201 aactgtttgc cgcctatgcg cgagctgata ccgatattct gatggggcag cggatgcgtt
    19261 cgttgcaaaa tcaggtcgtc gatatcgcgc cggagcaata tcagaaactg cgtcaggatt
    19321 tccttaacag cctgacggtg gagatgttaa atcaggatct gcgtcagcag ttgtcgaatg
    19381 atatggcgtt aatactgctg cagccgaaag gcgagccgga atttaacatg aaagcgttgc
    19441 aggcggcctg ggatcaaatc atggccccat cgactgcggc tgccgccaca tctgtcgcca
    19501 cggatgacgt acatcctgaa gtgacggata ttccacctgt acagtaatgc ctgatggtga
    19561 tgagttagca caaccatcag gtatttatgg atgagctgga tagaggggta acactctatc
    19621 cagccttttg catatgctgc ttttacgctg gcatcgcttc acgcgggata atcgcgccgc
    19681 gatactgaat aacggtactt gcagtcaggt gcccgcgttt cgctgcgtct tccgcactgc
    19741 cgcccgtcaa acgtaccgcc agataaccgg cactgaaaga gtcgccagct gcggtggtat
    19801 cgatcacttt ttctttcggc agttttaccg ccggaacatc cactaaccct tcaccagcaa
    19861 tggacaccag gcaagaatcc gccccgcgtt tcaccaccac ttctttcacg cccgcgttat
    19921 gggtgcgcgc aatgacgtct tccaccggct gttgacccca cagcgcgtct tcatcgtcca
    19981 gcgtcaggaa ggcgatatcc gtgcattcca gcatttgttg gtacacctgc tgtgtctctt
    20041 ctttgctggc ccacaggcgc ggacgatagt tattgtcgaa aatcactttt ccgccgttgg
    20101 cgcggcattc gcgcagcagg gaaagcagct tttcgcggct ggtcgggctt aagatcgcca
    20161 ggctaatccc gctcaggtag agataatcga aattcgccag ctcttcgcaa atcgccgagg
    20221 attgctcact ctccagccag aatttggcgg cggcttcgtt acgccagtag tagaacgtac
    20281 gttcgccggt gctgtcggtt tcaatgtagt aaaggcccgg cagacggttt tccatccgtt
    20341 gggtcaggga agtatcaacg ttctcgccgc gccaggcgtc cagcatctgc tggctaaaac
    20401 tgtccgttcc cagcgccgtt acgtaatgaa cggttaatgc cgcaggatcg acctgacggg
    20461 cgatatagac ggaagtgttc agggtatcgc cgccgaaacc gcgcttaacg tccgcgcctt
    20521 tctcggaaag ctcaatcatg cattcgccaa tcacggcaat ctttttggac atagtcgtga
    20581 acctgatctg taaaaaatta gcgttagttt gcgctgtggt ggtttgctgg tcaaccatat
    20641 taaaacagtg ttccattatt tttttgagcc agaacgtatt cctgaaagat tccgttgtgg
    20701 agtggggaaa tttaccccgg ccaggcggta aagttctgcc cttgcgcgcc gataatcttt
    20761 gtcgagtccg ggcagcatca cttttaaaca caggacatct ttgatgataa ggcaggttat
    20821 ccagcgaata agcaaccctg aagcaagcat cgagagcttg caggaacggc gtttttggtt
    20881 gcagtgtgag cgtgcttaca cttggcagcc aatctatcaa acatgcgggc ggttaatggc
    20941 cgtggagcta ttaacggtgg tcacgcatcc cttgaaccct tcgcaacgcc tgccgccgga
    21001 tcgctatttt actgaaatca ccgttagcca tcggatggag gttgtgaaag agcagattga
    21061 tttgctggcg caaaaagccg acttctttgt agagcacggc ctgctggcat cggtcaatat
    21121 tgatggccct acgctcatcg ccctgcgtca gcaaccaaaa atcctgcgcc agattgagga
    21181 tcttccctgg ctgcgtttcg aactggtgga acacatccgc ctgccgaaag attcaacctt
    21241 tgcctcgatg tgtgaatttg gcccgctgtg gctggatgat tttggtaccg ggatggcaaa
    21301 tttctctgca ctaagtgaag tgcgttatga ctacatcaaa atcgcgcgag aactgtttgt
    21361 gatgctgcgt cagtcgccgg aaggacgcac actcttttct cagcttttac atctaatgaa
    21421 tcgctattgt cgcggggtga ttgtcgaggg cgtagaaaca ccggaagagt ggcgtgatgt
    21481 tcagaactcg cccgcattcg ccgcacaagg ctggtttctt tcacgcccgg caccgataga
    21541 aacgctgaat acggcggttc tggcgctata agctgcctca ttttccgcct ggctggacta
    21601 tctttaggac tggcacagga agaatatgag gaagcgaacg atgagcaagg caggcaaaat
    21661 aaccgctgcg atttcagggg ctttcttgtt gttgattgtc gtggcgatca ttttgattgc
    21721 aacttttgac tggaatcgac tcaaaccgac catcaaccag aaagtctctg cggagttgaa
    21781 tcgtccgttc gctatccgtg gcgatctggg cgtggtgtgg gagcggcaaa aacaagaaac
    21841 tggctggcgc agctgggtgc cgtggcccca tgtacacgcg gaagacatca ttcttggcaa
    21901 tccaccggat attcccgaag tcacaatggt gcatttgccg cgagtagaag cgacgctggc
    21961 cccgctggcg ctgctgacca aaacggtctg gttgccgtgg atcaagctcg aaaagcccga
    22021 cgcgcgcctg attcgcctct ccgaaaagaa caataactgg acgtttaatc tcgccaacga
    22081 tgacaacaaa gacgcgaatg caaagccgtc ggcgtggttg tttcagctgg ataatattct
    22141 tttcgatcaa gggcggatcg ccattgatga caaagtaagc aaagcggatc tggaaatttt
    22201 tgtcgatccg ttaggcaagc cgctgccgtt cagcgaagtt accggatcga aaggtaaagc
    22261 ggataaagaa aaggtgggcg attacgtttt tggcctgaag gcgcagggac gttataacgg
    22321 tgaaccgctc actggtacgg gaaaaatagg cggtatgctg gcgctgcgtg gcgaaggaac
    22381 gccgtttccg gtacaggctg atttccgctc tggtaacacc cgtgttgctt ttgatggcgt
    22441 tgtgaatgac ccaatgaaga tggggggtgt cgatttacgg cttaaatttt ctggcgattc
    22501 gctgggtgat ctctatgaac tgacgggcgt tctgctgccc gatacaccgc cgtttgaaac
    22561 ggatggtcgg ctggtagcga aaatcgacac tgaaaaatcg tcggtctttg attatcgcgg
    22621 ttttaatggg cgcattggcg atagcgatat ccacggttct ctgatctata ccaccggcaa
    22681 gccacgacca aaactggaag gtgatgtcga gtcgcggcaa ttgcggctgg cggacctggg
    22741 accgttgatt ggcgttgatt ccgggaaagg ggcagaaaag tcgaaacggt ctgaacagaa
    22801 gaagggcgaa aaaagcgttc agcctgcggg taaagtgctg ccttatgacc gcttcgaaac
    22861 cgataaatgg gacgttatgg atgccgatgt tcgcttcaaa gggcggcgca ttgagcatgg
    22921 cagcagcctg ccgattagcg atctttctac tcatatcatc ctcaaaaatg ctgacctgcg
    22981 cctgcaaccg ctgaaatttg gcatagcggg cggcagcatt gcggcgaata ttcatctgga
    23041 aggcgataaa aagccgatgc aggggcgggc agatattcag gctcgtcgac tgaaactgaa
    23101 agaactgatg ccggatgtgg aactgatgca gaagacgctg ggggaaatga acggtgacgc
    23161 ggaactacgc ggtagcggta actcggtggc ggcgctttta ggcaacagta atggcaacct
    23221 gaaactgttg atgaatgacg ggctggtgag ccgcaacctg atggagattg tcgggctgaa
    23281 tgttggtaac tacattgtcg gtgcgatatt tggtgacgat gaggtgcggg tgaactgcgc
    23341 ggcagcgaat ctggatattg ccaacggcgt ggcgcgcccg cagatttttg ctttcgatac
    23401 tgagaacgcg ttgattaacg ttaccggcac ggcaagtttt gcttcggaac agctggattt
    23461 aactatcgat ccagaaagta aagggattcg cattatcacg ctgcgttcgc cgctgtatgt
    23521 gcgtgggacg tttaaaaatc cgcaggctgg ggtgaaagcc gggccgttga ttgctcgcgg
    23581 agccgttgcg gcggcactgg caacgctggt aacaccggcg gcggcgttac tggcgctgat
    23641 ctcaccttcc gaaggggagg ctaatcagtg caggacaatt ttgtcgcaga tgaaaaagtg
    23701 atgataattg ccggatgtct gacatccggc agttaaaacg actacaacga ctggtgccgc
    23761 gtctcatgcg ttaacagcag ggcaattaac gtcagtccgg ccatcgccgc cagatataac
    23821 cccaccgcgc ctaacccata gttagtctgc aaccaggccg cgatatacgg cgcaacagac
    23881 gccccgagaa tcgacgatac gttataggag aacgatgctc cggtataacg gacttccgtc
    23941 gggaacagct ccggcagcag cgcccccatc ggaccaaagg tcaggcccat caaactcaag
    24001 cccagcagca ggaaaacaaa caccaatgct ggattaccag aacccaacag tgggttaaag
    24061 gcaaacaggg caaacagaat gataagcgta gtgatcacca ccatactttt acggcgacca
    24121 aaagcatctg ccagcagacc cgccaacgga accatcactg caaaaccgat aaccgccatc
    24181 atcagcatcc acaacacttc gttacgcggt aaaccaagcc caactggcgc ggcggcggta
    24241 ctaaaggtca tagagtagac cgtcatgatg taaaacagcg tataggttgc cagcataatg
    24301 aacgtaccca gtacggttac gcgaacatgt ttggtcagca gcgtacccag cgggatcttc
    24361 acctgttttt tcgctttagc gactttctca aacaccggcg actcatgcag cgacacgcga
    24421 acatacaggc cgataatgac cagcaccgcc gagaagataa atggcacacg ccagccccag
    24481 ctcataaact gctcgtcggt cagtagccag gaaagcagca agaaagtgcc gttggcaaag
    24541 aagaagccaa tcggtgcgcc cagctgcggg aaggagccgt acaatgcacg tttgcgcggt
    24601 ggggcgtttt cagtcgccag cagcgccgcg ccgccccatt caccacctaa gcccagaccc
    24661 tgaccaaatc gagccagcgc cagcagcagc ggggcgaaaa taccaatcgt ggcatagcct
    24721 ggcagcagac caatcaccac ggtcgaaatc cccatcgtta gcaacgaggc gaccagcgtc
    24781 gctttacgcc caacgcgatc gccaaaatga ccaaaaacgg cagagccaat ggggcgcgcg
    24841 acgaaggcga tggcgaaggt ggcgagcgac tgtagcgttg ctgccgttgg atcgccctgc
    24901 gggaagaaga tatgcggaaa cacaataacg gccgcagtgg cgtaaatgta aaagtcgaag
    24961 aactcaatgg ccgtgccaat gagagaggcg acaaggactt tattacgcga gttgatcggc
    25021 gtgtattctt gctcgtggtc gagtgttgtg gctgttgctt gcataatctt ttcttatttt
    25081 ttggctaacg aatagccata ttacgcacag caaaagtgat atttcaatgt gtaacaaatc
    25141 gcggaaatgg tcaaaaatca tgcaaaaagg ggataatgtt taggccagtg atttttcttc
    25201 tatgaaatgc ttcacataat tttataaatg gtggattaaa ctggttttag gttaaataaa
    25261 agttaccaac tggatttata tgctggggtt atgagttgga atgaaattgt ttgctcctga
    25321 atagatggct aaagaggctc tgggaaagag tttcaagagg ttatgtgcgc attatagccg
    25381 gatgcgatgc tggcgcatct tatccggcct acggggagcc aggccgaata aattcgtacg
    25441 ccgcatccgg cctattttaa ggctgcgttt tccccggcat tcgcgggtcg tctttatatt
    25501 cggcggtggc aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag tagaaaaatg
    25561 ccatcagccc cagcacggaa ccaaatgctg cgccagaggg tgatttcatc aacgatggca
    25621 gggtgtaggt catcacgatt ttaatcactt caaaaccaat agcagccaga aatgttccgc
    25681 ggatcagggc ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc cagaagaaaa
    25741 gcagatagtt ggcgaagatg gaaatcgcca ggccaatcaa ccgccatgtc ggtttcagcc
    25801 actcaatgct gttcaggtgc agggcgctaa taatcatttg ctgcgcagaa ccggcaaccg
    25861 aggtgataga aagcgtcaca atcagcgcaa tcaacaaacc aatcagcgaa ataaaatcac
    25921 gcagatattt tacccagaac ttctcctgat cttgcggcga gcgttcccag acatcgcgtg
    25981 actgggcgcg aatcgcttca cgcaggttac ccatccagtt gatgccggaa taaagcgcca
    26041 ctgccagacc gacaagccct acagtcgtac gctgctgaac ggcggtgttg atggtgtttt
    26101 tcaacgtggc ggctagcgtc ggatcgctaa tgttttgcag aattttgtcg aagatatcct
    26161 gtagcaacat cggatgggag gccagcacaa agccccctgc ggcaaacgac accatcaaaa
    26221 tggggatcat cgacaaaaat gagaaatagg tgatagccgc cccaaactgg ttacccagcc
    26281 gatcgttaaa gcgttctgtc gcgcgaatca ggtgcgcaat aaccggttgg cgttggactt
    26341 tctcggcagt ggtggtgacg gtttccagcg cctggctaac tttgccgcct ttctcgctat
    26401 tatccaactg cttaatcggc tcgtgctcca gatcctgggt gggacgtttg atctcgtttt
    26461 cctgcgtcat ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt atagtcggtc
    26521 agtccaggtg ctctttcatt acgccagcca gccactgcat aaacaggttt acacgccggg
    26581 aaagctcccg acgctgcgga taaaccagtg aaagggacag cggctcggca cggtagccag
    26641 gcaacacttc aataagccgc ccggcacgca gggcttcgcg cacggcaatg cgcgggatct
    26701 gaataatccc cagaccggca agaccggcgg cgagatagtt ttcgctactg tttaccgtca
    26761 acatgccgcc agacttaaac cactggacgc cattaacgct ggcaacctca aaacctaacg
    26821 gatgtacacc caggtgcggt gtataacgca ctattgcgtg tgaagtcaga tcatcggggc
    26881 tttgcggata accaaagcgc gtcagatagt gtggactggc acagttgacc atggtcagtt
    26941 tgccgagaga acgggcgata acgccatctt ccggtaatgc gccagtgcgt atcacgcaat
    27001 caaaaccatc atgaagaata tctaccggac ggtcatggct actcagttcc agctcaattc
    27061 ccggatgcag atagagaaat tccgacaagc gcggcagtaa caggcttttc gcgattcctg
    27121 acgggatgtc gatgcgtaat ttaccgctga tactggtggc atcctgttga aacagaccgt
    27181 ccagttcgct gagattactc aacacatctt ttgctcgttg ataataggtc atgccttctg
    27241 gcgtgagttt gacccgtcgc gtggtgcgct ggagaagctg ggtgccaagt tgatgttcca
    27301 gtgcctgtat ctggcgcgaa acacttccct ttggcaaagc aaagaaatcc gccgcgcggg
    27361 aaaaactttc cagctccgcg actttgatga acaactgcat tgcgtgaatt ttatccattt
    27421 ctttgaccga ttgttgttca caacgaaaca gtagcgcgca tatcgcattg tttattgttt
    27481 tttctaacat ctaatgccgg gtttcacaat gttgtcatga tgaatgtggc gatttctcag
    27541 caggaaaagg gaggaatagc tttatgagaa aggtgaattc ccttaaacag agcgtatttt
    27601 tcagcgattc cttttaaagt gaatagaaga tgtattggct atttgtgctt atttttatct
    27661 cattgccgtt tgttattttt gtttatggtc gcggaatttc atttttaatt gcatgcgtac
    27721 taactaccga tataaggaaa tcctcaaaaa tgttttaaat agtgccctcg acatgtaatt
    27781 tcaggcgttt agctgtaact atttatatcg gtaactattt tctacgcgtc atctctgtag
    27841 aatggccacc gccaaatggc gaattcttat tttttctcgc gggtaatacc cttccactac
    27901 ttaagtcttt ttaacgtatt caggtggaga ttcagttatg caaatagtca tgtttgacag
    27961 gcagtcaata tttattcatg gaatgaaaat cagtttacag cagcgtattc caggagtgag
    28021 tattcagggg gccagtcagg cagacgagtt atggcaaaag ctggaaagtt accctgaagc
    28081 cttagtgatg ctcgatggcg atcaggatgg tgagttttgc tactggttgc tgcaaaaaac
    28141 cgtggtgcaa tttcctgagg ttaaggtgtt aattacggcg acggattgca acaaacggtg
    28201 gttacaggaa gttattcatt ttaatgtgct ggccattgtg cctcgtgatt caaccgtcga
    28261 gacatttgcg ttggcggtaa atagtgcggc gatggggatg atgtttctgc ctggagactg
    28321 gcgaacgacg ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc gccagcggga
    28381 gattttaacc atgttagcgg cgggcgaatc aaataaagag atcggcaggg cgctgaatat
    28441 cagtaccgga acggtaaaag cgcatctgga atcactgtat cgccgtctgg aagtcaaaaa
    28501 tcgcacccag gcggcaatga tgttaaatat ctcctcctga agaggataat tttacagcca
    28561 gcaggaagtt agcggctggc tgtaaaaata ttatggttcg ccgtacaaac caattaaacg
    28621 gcgtaccaca ccgttagtcc agccaaaccc atcctgcaac ggatactcgc caccgccgcc
    28681 ttcgcgagga acaccatcgg caatatggta tttctcgatc agtttgtgct gttccagata
    28741 gaactgattc accgtcttca gccagttgcg cgcgatttca tcacccagaa ggtcatcgcc
    28801 gtacatctta aatccctgaa ttgccatcca ctgtaacggt gcccagccgt tgggtttatc
    28861 ccactgttca ccggtttcgt actcgcttgc cagaatcccg ccaggtgtca gtaaccggct
    28921 gcgcacggcg tttgccagac gatcggcctg ttcatggttc gccataccga catagagcgg
    28981 cacaatggcg gcagcggaaa acagcgccag ttgctcgcgt cgccagtcgt aatcgcggta
    29041 gatgccgttt tcatcatccc agaggtaacg gtttaccgca tcgcgacggg cactggcctt
    29101 ctggcggaac agtgcttctg tctctttctc gcctttcagc gccgagatgt tggcgatggc
    29161 gctctccagt ttaaacagga aggcattcag atcgatgggg atgaactggg tggtacgaat
    29221 gctcgccaga cgaccagtat cacgcagcca acgggaagag taatcccaac cggaggccgc
    29281 ccccgcacgt aaatcgcggt acacctcgtt aggcgggcga ccagaatgtt tcgcggtttc
    29341 aacgtcctca agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccagt aacggttgag
    29401 cagcgagccg tccggcatcc gcacaacatg gcgataggcc tgattaggga tcaacgattc
    29461 tgcaccgtcc atccagaagg catattccat tttcaggtgg tcgagatagc ggcgcgcgcc
    29521 gcgtacacca tcttcttcaa acaactccac catcagcgca aaaaccggtg gttgcgagcg
    29581 gctcaaataa taggtgcggt tgccgttggg gatgtggccg tagttttcga tcatccaggc
    29641 gaagttatcg gccatgcatt tcagcaaatc ttcccgacca ctttccgcca gccccagcat
    29701 ggtgaaatag gaatcccaat agtaggtttc gctaaaacgg ccgcccggga caatatatga
    29761 ctgcggcagc gccagcagag aagaccacgg aatgtgatcc tgtggttcac gggttagcac
    29821 cggccacagc tggtcgatat gctctttcag ggaattttgc gggtccgata catactcgct
    29881 ggagtagacc tccggcagcc agaagtggtt ttcaacaaac ttgcgcaagt caaaatcacg
    29941 atgacggcgc actttacggt agcggattaa aatatccagc gggtccattt tgggtgcgca
    30001 gtcgggaaag gttttactgt cggggaaaat tttcgccgac tgaacatgct cgaacagttc
    30061 gagatagcga tcggcaggcg tcagcgcatc agaggcgggc agcccttcaa tcatctcggg
    30121 ttccggttct gcctcgatca tctcatccag ttttaattca tacgggtcca gctcatagca
    30181 gagatcgacc tcgatcatca gttcgtctgg attagggttt tgaattttct gattgagcat
    30241 aaggatccga cctccgaaat tcgtcgctaa ttatcgggct tttctttaag ggtagtcatt
    30301 cgctccgcct ggtgggcgac aaagcgtgcg gtggatcacg tttattaacc tgccaaaaaa
    30361 tataacgcac taatatcaat ggaatttata aaaatacgga tttcgtgttg tttttcattt
    30421 tcagaacatt caatcctgct ctcgccgcaa tcatgatatg ccattcctga tctgggtaat
    30481 aggcactggc tatcttattg atagtttata ttcatgtaat tgattgattc ctatcaaaaa
    30541 gcatatctcc ctgtttttta tgatggcttt gtctcgcaga gacgtttgca gcattctgga
    30601 aaattttctg ggtcacaata actcaccacc gtgagggaag aataatgaaa atggtctcac
    30661 gtattaccgc gatcggcctg gctggcgtcg cgatttgcta tttagggtta tctggttatg
    30721 tgtggtacca cgataataaa cgcagtaaac aggccgatgt tcaggcatct gctgtcagtg
    30781 aaaataataa ggttttaggc tttctccgcg aaaaaggatg cgattattgc catacgcctt
    30841 cggcagaatt acctgcctat tattatattc ccggcgcgaa acagttgatg gattacgaca
    30901 taaagcttgg atataaatct tttaacctcg aggccgtgcg tgcggctctg ctggctaata
    30961 aacccgtttc gcaaagcgat ctgaataaga ttgaatgggt gatgcagtat gaaactatgc
    31021 cgccaacgcg ttataccgcg ctacactggg cgggtaaggt gagtgatgaa gagcgggcgg
    31081 aaatactggc ctggattgca aaacagcgcg cggaatatta cgccagcaat gatactgctc
    31141 cggagcatcg caatgaaccg gtgcagccca tcccgcaaaa actgcctacc gatgcgcaaa
    31201 aagtggcgtt gggctttgcg ctgtatcacg atcctcgttt atcggctgat agcaccattt
    31261 catgcgctca ttgccatgcg ttgaatgcga gaggcgtcga tggtagaaaa acatcgattg
    31321 gtgttggtgg cgcagttggg ccgattaacg cgccgacggt atttaactca gtatttaacg
    31381 ttgagcagtt ctgggatggt cgtgcggcaa cattgcagga tcaggctggt ggaccgccgt
    31441 tgaacccgat tgaaatggcg tcgaaatcct gggacgaaat tattgctaag ctggaaaaag
    31501 atccgcagct taaagcgcag ttcctcgaag tctatccgca aggtttcagt ggcgaaaata
    31561 ttactgatgc cattgctgaa tttgagaaaa cattaattac gccggattcc ccatttgata
    31621 aatggttgcg tggagatgag aatgctctga cggcgcaaca gaaaaaaggc tatcaattat
    31681 ttaaagataa taaatgtgca acttgtcatg gtggtattat tctcggtgga cgttcctttg
    31741 aaccgttggg gctgaaaaaa gactttaact ttggtgaaat tacggcggcg gatattggtc
    31801 gtatgaatgt tactaaagaa gagcgtgata aattgcgtca gaaagtaccc ggtttacgta
    31861 acgttgcttt aacggcaccg tacttccatc gcggtgacgt gccgacgctg gacggggcgg
    31921 tgaaactgat gctgcgctat caggtaggca aagagctgcc gcaggaagat gtggatgata
    31981 tcgtagcttt cctgcacagt ctgaacgggg tttacacgcc gtatatgcag gataaacaat
    32041 aattaatttg atcgcccga
//
LOCUS       DADSZD010000035        31243 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.035, whole genome shotgun sequence.
ACCESSION   DADSZD010000035 DADSZD010000000
VERSION     DADSZD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.035"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            91..3861
                     /gene="yfaL"
                     /locus_tag="J1Z28_001487"
     CDS             91..3861
                     /gene="yfaL"
                     /locus_tag="J1Z28_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBA7716332.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMANNASGGAVFLQHSAEFTISPQNETGITLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTDAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATATAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEMSIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGDTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSASDQLVLNGNTAGNTAVV
                     INPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDATPDPTPDPEPTPAYQPVLNAKVGGYFNNLR
                     AANQAFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFSGHW
                     GDDGEWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLD
                     SWLQYVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQ
                     DDFTAANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHSTGIEEDGST
                     ISDDAAKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4001..4723)
                     /locus_tag="J1Z28_001488"
     CDS             complement(4001..4723)
                     /locus_tag="J1Z28_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBA7716333.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LSYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4870..7497
                     /gene="gyrA"
                     /locus_tag="J1Z28_001489"
     CDS             4870..7497
                     /gene="gyrA"
                     /locus_tag="J1Z28_001489"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBA7716334.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7646..9334
                     /locus_tag="J1Z28_001490"
     CDS             7646..9334
                     /locus_tag="J1Z28_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBA7716335.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPKKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPAMVDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYYAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9331..9954
                     /locus_tag="J1Z28_001491"
     CDS             9331..9954
                     /locus_tag="J1Z28_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBA7716336.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQFRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10098..14492
                     /locus_tag="J1Z28_001492"
     CDS             10098..14492
                     /locus_tag="J1Z28_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBA7716337.1"
                     /translation="MRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRI
                     VVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQALPELQLGNAIIKP
                     SRYVQNSQFSPLKKYPLVEQFRYPLWQATSVEPQQGVKLEGASSNFISPQPGNIYIPL
                     GKQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEIL
                     WTDGLGVMTRGVTDGSGTLQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLY
                     IFTDRPLYRAGDRVDVKMMGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDA
                     RNGGQGSFRLPENAVAGGYELRLAYRKQVYSSSFRVANYIKPHFEIGLALDKKEFKTG
                     EAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDDN
                     GHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYTLSTAAQYSNSGE
                     SVVFRYAVLESSKQVPVTYEWLRLEDRTSHSGDLPSGGKSFTVNFDKPGNYNLTLRDK
                     DGLILAGLSHVVSGKGSTSHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLER
                     DRVEQQSLLSHPANWLTLQRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAG
                     IKVAVPQLDIRVKTDKTHYQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEI
                     APNIGKFFYPLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREE
                     VDTAAWMPSLTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKSLY
                     MKWSMPTVYRMGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQ
                     NIQQSGLLSAELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGDNALMLPEQASN
                     IRLQSSETPQEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQ
                     MIQDNRLRLMQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQH
                     MLDSYAEQADNMPLLHRALVLAWAQEMNLPCKTLLKGLDEAIARRGTKTKDFSEADTS
                     DINDSLILDTPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAH
                     TVVLLNSGGDATQAAAILSGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAK
                     HKLTGGGEYWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYR
                     LIPGEEEMSFTLQPVTSNEIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERT
                     TWGISVNKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRYLLRFSQKGQFVL
                     PPARYVRSYAPAQQSVAAGSEWTGMKVK"
     gene            14493..16149
                     /locus_tag="J1Z28_001493"
                     /pseudo
     CDS             14493..16149
                     /locus_tag="J1Z28_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2300 domain-containing protein"
     gene            16154..16930
                     /locus_tag="J1Z28_001494"
     CDS             16154..16930
                     /locus_tag="J1Z28_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBA7716338.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     SRPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17004..18188)
                     /locus_tag="J1Z28_001495"
     CDS             complement(17004..18188)
                     /locus_tag="J1Z28_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBA7716339.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLNPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18219..19541)
                     /locus_tag="J1Z28_001496"
     CDS             complement(18219..19541)
                     /locus_tag="J1Z28_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBA7716340.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19538..20188)
                     /locus_tag="J1Z28_001497"
     CDS             complement(19538..20188)
                     /locus_tag="J1Z28_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HBA7716341.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20188..20850)
                     /gene="atoD"
                     /locus_tag="J1Z28_001498"
     CDS             complement(20188..20850)
                     /gene="atoD"
                     /locus_tag="J1Z28_001498"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBA7716342.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21046..22431)
                     /gene="atoC"
                     /locus_tag="J1Z28_001499"
     CDS             complement(21046..22431)
                     /gene="atoC"
                     /locus_tag="J1Z28_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBA7716343.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22428..24254)
                     /gene="atoS"
                     /locus_tag="J1Z28_001500"
     CDS             complement(22428..24254)
                     /gene="atoS"
                     /locus_tag="J1Z28_001500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBA7716344.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24421..27270
                     /gene="rcsC"
                     /locus_tag="J1Z28_001501"
     CDS             24421..27270
                     /gene="rcsC"
                     /locus_tag="J1Z28_001501"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBA7716345.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHILISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVNKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27470..28120)
                     /gene="rcsB"
                     /locus_tag="J1Z28_001502"
     CDS             complement(27470..28120)
                     /gene="rcsB"
                     /locus_tag="J1Z28_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBA7716346.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28137..30809)
                     /gene="rcsD"
                     /locus_tag="J1Z28_001503"
     CDS             complement(28137..30809)
                     /gene="rcsD"
                     /locus_tag="J1Z28_001503"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBA7716347.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLPLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         7685 a         7638 c         8376 g         7544 t
ORIGIN      
        1 atcaggggca gaatttacaa aagaaaaata ccctatatcg cataatgcga ttaaatacgc
       61 catcttatag aaaatgatgg ttagatgaat atgcggatta tctctctacg caaggagtat
      121 ttatctttac tcccgtcaat gattgcatct cttttctctg ctaacggtgc cgcagcggtc
      181 attgattcat gccagggata tgatatcaaa gcgagctgtc aggccagcag gcaaagcctt
      241 tcgggcaata tacaggactg gagcatcgcg gatgggcaat ggttgatttt ttcgggtatg
      301 gccaataatg ccagcggtgg cgcagtattt ttgcagcaca gtgctgaatt tacgatatca
      361 ccacaaaatg aaacagggat aaccctgttt gctgataact cggttagtgg cgaatataat
      421 aatggtgggg ctatctttgc taaagaaaac tcaacgataa atcttgcgaa tgttattttc
      481 gacagtaatg ttgcaggagg ctatggcgga gcaatctatt ctgccggtac caatgatacc
      541 gatgccgccg atttacgtgt cactaacgcc gtatttcaca ataacatcgc taatgacggc
      601 aaaggcggtg caatttatac catcaataat gacgtctatt taagtgatga tgcttttaat
      661 aataaccagg catatacatc aacaagttat agtgatgggg atggcggcgc tattgatgtt
      721 acggataata gtacggataa cacacatctt tcaggaaaaa cgataattaa taatacctcc
      781 tttaccaata actatgcaga aggttatggc ggagcaattt ataccagcag caccacatcc
      841 ccgtatctta ttgatatttc tgttgatgat aattatgacc aaaacaatgg tgtgatgatt
      901 gatgaaaata acagcgcctc gggatatgac cattcggcga cggcaacggc ggggggcttt
      961 atgtatatcg gtcatagtgt ggctgaattt aacattgccg ccgataaaac gctggtaata
     1021 ggcaatacca gcaacgatgg cgcgattgat tctcttgccg gaacgggagt catcgtgaaa
     1081 gagggagcgg gagagttagt cctcaatgcc gacaataatg cgtttaccgg cgagatgagt
     1141 atccagaatg gcgaggtgac gttggggcgc agcgatgagt taatgaatgt cggcgacacg
     1201 cactgtcaga gcgatccgca ggattgcttt ggtttgatgg tcggcagcac tgttcattct
     1261 gagtatcagg cagaactgaa tgttggcaat acacaacaaa cgtttgtgca ctcattaacc
     1321 ggttttgcta atggcatttt gaatatcgac gcaggcggca atgttaccgt aaaccagggc
     1381 ggtttttccg gctcgattca gggtgaagga cagttgacgg tagcgcaaga tggcagctat
     1441 ctcctgacag gggcacagtc gatggcatta accggcgata ttgtggtgga agataacgcc
     1501 gtattgtcgc tggcggggaa tcaggccgat ttacgggcaa tgcagagcga tccgcaatcg
     1561 atcgtgctaa atggcggagt attggatctc tctgatttca ccacctggga tggcgatagc
     1621 tcatacaatg acggtctgca aatcagcggc agcgggggga cagtgattgg cagtaatgat
     1681 gtcgttgata tcagcagtgg tgatgattta catattggcg gcagtgatgc cagccagaat
     1741 ggcgtttatg tcgtcattaa tgcgggggat cagcgcgtaa cgctggcgaa taacaacggt
     1801 tatctcggta atacgcaaat cgcctccggt acgcttgaag tcagcgataa ttcgcaattg
     1861 ggggacacaa gttacaaccg ttcagtgatc ttcacagatc cccaacaaca cagtgaaatg
     1921 gatgtgacga ctgatgttga cactcgctcg gctacaacag gtcagggcag gaatattgaa
     1981 atgcgcgctg atggcgaaat acacgttgag gatggcgtag atacgcaatg gggcggattg
     2041 atggcggata gtaccgggca acaactggat agcgtaagca cgttgactaa aagtggcggc
     2101 ggtacgctgg agttgaccgc cagcggcact gcgacgtctg cggtacgtgt ggaggacgga
     2161 acgctaaaag gtgaagcgga gaatattatt ccttatgttt cttcactgtg ggtgggggaa
     2221 gacggtgttt ttgaaaccgg gcaaaatcag gatattcgtt caatcgatgc cacctctggc
     2281 ggcgatatcg acataaccga tggtactgtg ttgcgattga cgcagcagga tacgaaccag
     2341 gcgctggatg cctcgctgtt tagcggcgac ggtacgctgg tgaatgccac cgatggcgtg
     2401 acgctggcag gtgagcttaa taccaacctt gaaactgaca gcctgactta tctttccgac
     2461 gtgacggtta atggcaatct gaccaatacg tccggtgcga ttagcctgca aaatggcgtc
     2521 gctggcgaca cgctgacggt aaatggtgat tataccggcg gcggtacgct actgctcgat
     2581 agcgaattaa acggcgatga ctcagcaagt gaccaactgg tattgaacgg taatactgct
     2641 ggcaacacgg ccgtggtaat taaccccatt acgggtattg gtgagccgac atctacaggt
     2701 attaaagtag ttgatttcgc agctgatccc gcgcagtttc aaaacaatgc gcagttcagt
     2761 ctggcaggaa gcggctacgt caatatgggg gcgtatgact acacgctggt ggaagataac
     2821 aacgactggt atctgcgatc acaagaagtc aatccgacgc cgccgcctga tccagacccg
     2881 actccagatc ctgatcccac gccggatccc gacgcaaccc ccgacccaac ccccgacccg
     2941 gaacctacgc ctgcctacca gccggtgttg aatgccaaag ttggtggata ttttaataac
     3001 ctgcgggcgg caaatcaggc atttgtgatg gagcgacgcg atcacgctgg tggcgatggt
     3061 cagacgctga atttacgcgt tatcggcggg cgttatcatt acacggcggt agggcagttg
     3121 gctcagcatg aagacacttc tacggtgcag cttagcggca acctgtttag cgggcactgg
     3181 ggtgatgatg gcgagtggat gcttgggatt gttggtggct acagcgataa ccagggcgac
     3241 agccgctcga atatgaccgg aactcgcgcc gataaccaga accacggtta tgccgttggg
     3301 ctgacctcaa gctggtatca gcacggtaat cagaagcagg gggcctggtt ggatagctgg
     3361 ctgcaatatg tgtggtttaa caatgatgtt tccgaacaag acgatggcgt ggatcattac
     3421 cactcgtcgg ggattatcgc ctcgctggaa gccggttatc agtggttacc ggggcatggt
     3481 gtggtgattg aaccgcagac gcaggtgatt tatcagggcg tgcagcagga tgattttacc
     3541 gccgctaacc atgcgcgcgt gtcacaatcg cagggtgatg atattcagac gcggctgggt
     3601 ttacacagcg aatggcgtac tgctgttggt gtcacgccaa cgttagatct gaattattat
     3661 cacgatcccc attcgacggg aattgaagaa gatggcagca ctatcagtga cgatgcggcg
     3721 aagcaacggg gtgaaataaa agtgggaata acgggcaata tcagtcagcg agtttcgctg
     3781 cgtggtagcg tggcgtggca gaaagggagt gatgattttg cccagacggc agggtttttg
     3841 tcgatgacag tgaaatggta atcgctgtct gaccggcact gttgccggat gcggcgtaaa
     3901 cgccttatct ggcctacggg gagcatttgt aggcctgata agacgcggca agcgccgcat
     3961 caggcttggc tcggttgccg gatgaatcca aacaacaacc tcacttattc tgcgtgtgca
     4021 gcatatagtt cacatccacg ccggggccga gcttaaagga attagtgata gggttgtaat
     4081 gcagcccggt catatgccgt tctttaagac tggtctgatc cacccagccc agcaattctg
     4141 ccggtttaat aaacttcttc acatcatgtg tacctttagg caccatgcgc aaaatatatt
     4201 ccgcaccaac caccgccatc agccatgact tgccgttgcg gttaagcgtc gagaaaaaga
     4261 catcgccgcc cggtttcacc agttgcgcac aggctctgac cactgactga ggatcgggga
     4321 cgtgctccag catctccata caggtcacca cgtcatactg tccggcatgt tttgccgcgt
     4381 gctcttccac ggtttcctgc acgtaatcca cctgaatgcc gctttccagt gcgtgcagtt
     4441 ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg ccctcccgcg
     4501 ccatactctc ggccagaata ccgccgccac aaccgacatc gagcaccttt ttgccaaata
     4561 aaccgccagc acgctcggca atatagctca gacgcagcgg gttaatgcgg tgcagcggtt
     4621 tgaactcacc ttcaagatcc caccagcggg aggcgacggc ttcaaattta gcgatctctt
     4681 cgtggtctac gttgtggttt accggcgatt tttcggcatt cattggcact tctactccgt
     4741 aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta taggtttacc
     4801 tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca gtagagggat
     4861 agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga ggaagagctg
     4921 aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct gccagatgtc
     4981 cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt actaggcaat
     5041 gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat cggtaaatac
     5101 catccccatg gtgactcggc ggtttatgac acgatcgtcc gtatggcgca gccattctcg
     5161 ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg cgactctgcg
     5221 gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact gatggctgat
     5281 ctcgaaaaag agacggtcga tttcgttgat aactatgacg gtacggaaaa aattccggac
     5341 gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt cttccggtat cgccgtaggt
     5401 atggcaacca acatcccgcc gcacaacctg acggaagtca tcaacggttg tctggcgtat
     5461 atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg gccggacttc
     5521 ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg taccggtcgc
     5581 ggcaaggtat atatccgcgc ccgtgccgaa gtagaagttg acgccaaaac cggacgtgaa
     5641 accattatcg tccacgaaat tccttatcag gtgaacaaag cgcgcctgat cgagaagatt
     5701 gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga cgagtctgac
     5761 aaagacggta tgcgcatcgt gattgaagtg aaacgcgatg cggtcggtga agttgtgctc
     5821 aacaacctct actcccagac ccagttgcag gtttctttcg gtatcaacat ggtggcattg
     5881 caccatggtc agccgaagat catgaacctg aaagacatca tcgcggcgtt tgttcgtcac
     5941 cgccgtgaag tagtaacccg tcgtactatt ttcgaactgc gtaaagctcg cgatcgtgct
     6001 catatccttg aagcattagc tgtggcgctg gcgaacatcg acccgatcat cgaactgatc
     6061 cgtcatgcgc cgacgcctgc agaagcgaaa accgcgctgg ttgcaaatcc gtggcagctg
     6121 ggcaacgttg ctgcgatgct ggaacgtgcg ggcgacgatg ctgcgcgtcc ggaatggctg
     6181 gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca agctcaggcg
     6241 attctggatc tgcgtctgca gaaactgacc ggccttgagc acgaaaaact gctcgacgaa
     6301 tacaaagagc tgctggatca gattgcggaa ctgttgcgta ttctgggtag cgccgatcgt
     6361 ctgatggaag tgatccgcga agagctggaa ctggttcgtg aacagttcgg tgacaaacgt
     6421 cgtaccgaaa tcaccgctaa cagcgccgac atcaacctgg aagatctgat cacccaggaa
     6481 gatgtggtgg tcacgctttc tcaccagggt tacgtgaagt atcagccgct ttctgaatac
     6541 gaagcgcagc gtcgtggtgg gaaaggtaaa tctgccgcac gtattaaaga agaagacttt
     6601 atcgaccgac tgctggtggc gaacactcac gaccatattc tgtgcttctc cagccgtggc
     6661 cgcgtctatt cgatgaaggt ctaccagttg ccggaagcca ctcgtggcgc gcgcggtcgt
     6721 ccgatcgtca acctgctgcc gctggaacag gacgaacgta tcaccgcgat cctgccggtg
     6781 accgagtttg aagaaggcgt gaaagtcttc atggcgaccg ctaacggtac tgtgaagaaa
     6841 accgtcctca ccgagttcaa ccgtctgcgt accgccggta aagtggcgat caaactggtt
     6901 gaaggcgatg agctgatcgg cgttgacctg accagcggcg aagacgaagt aatgctgttc
     6961 tccgctgaag gtaaagtggt gcgctttaaa gagtcttctg tccgtgcgat gggctgcaac
     7021 accaccggtg ttcgtggtat tcgtttaggc gaaggcgata aagtcgtctc tctgatcgtg
     7081 cctcgtggcg atggcgcaat cctcaccgca acgcagaacg gttatggtaa acgtaccgca
     7141 gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg ttatctccat caaggttacc
     7201 gaacgtaacg gtttagtggt tggcgcggtg caggtagatg actgcgacca gatcatgatg
     7261 atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg aaatcagcat cgtaggccgt
     7321 aacacccagg gcgtaatcct tatccgtact tcggaagatg aaaacgtggt gggtctgcaa
     7381 cgtgttgctg aaccggttga cgaggaagat ctggatacca tcgacggcag tgccgcggaa
     7441 ggggacgatg aaatcgctcc ggaagtggac gttgacgacg agccagaaga agaataattt
     7501 catcgcttca tgccaaaagg gagccatctc ccttttttga attgaaaagt ccaggctgta
     7561 aagtctgggc ttttgtcgta ttagggcgcg gtaaagtttg gctgtgcccg taaaaaatgg
     7621 ctggctatac acaaggaatg tggcaatgag tggtgaaaaa aaggcgaaag gctggcggtt
     7681 ttatggcctt gttggttttg gggcaatagc gctgctatcc gctggcgtat gggcgttgca
     7741 atatgctggc agtgggccaa aaaaaacgtt gtcgccactg gtggtgcaca acaatctgca
     7801 aatcgatctc aatgagccgg acctctttct cgacagcgac tctctgagcc agcttcccaa
     7861 agatctcctt accattccgt ttctccacga tgttctgagc gaagatttcg ttttctatta
     7921 tcagaatcat gccgatcgtc tgggcattga aggcagcatt cgccgcattg tctatgaaca
     7981 cgatctcacg ctgaaagata agctcttttc gtcactctta gaccagcccg cgcaggcggc
     8041 actgtggcac gataaacaag gccatctttc tcattatatg gtgctgatcc agcgcagtgg
     8101 tttaagcaaa ctgctggagc cattgttgtt tgccgctacc agcgacagcc agttaagcaa
     8161 gacggaaatt agtagcatca agataaatag tgaaactatt cctgtttatc agttgcgcta
     8221 taacggcaat aacgtcctga tgtttgcgac ttatcaggac aaaatattgg cgttttccag
     8281 ttcggatatg ctgtttaaag atgaccaaca ggataccgaa gccacggcga tcgcaagtga
     8341 tttgttgagt ggtaaaaaac gctgggaaac ccgctttggc ctggaagcgc gtactgccga
     8401 aaaaacgcca gtacgccagc gcatcgtagt cagcgccagg ttgctggggt ttggctacca
     8461 gcgattaatg ccttcttttg ctggcgtacg cttcgaaatg ggtaacgacg gctggcacag
     8521 ttttctggcg ttaaacgatg aatccgccag cgtagatgcc agtttcgatt ttacgccggt
     8581 atggaacagt atgcctgccg gagccagctt ctgtgtggcg gtgccgtatt cacacggtat
     8641 tgccgaagag atgctttcgc acatcagcca ggaaaacgac aagctgaatg gggcgttaga
     8701 cggtggcgcc gggctgtgct ggtatgaaga ctcaaaatta caaactccgc tatttgttgg
     8761 tcagtttgat ggcactgccg aacaggcgca attgccaggg aaactgttta cgcaaaatat
     8821 tggtgcgcac gaaagcaaag cgccagaagg tgttttgccg gtaagccaga ctcagcaggg
     8881 cgaagcgcaa atctggcgtc gcgaagtgag ttcccgatac ggtcagtatc cgaaagcgca
     8941 ggcggcgcaa cccgatcaat taatgtcgga ttattttttc cgcgtgtcgc tggcgatgca
     9001 aaacaaaacg ctgcttttct ccctcgatga cacgctggtt aataacgccc tgcaagcact
     9061 gaataaaacc cgcccggcaa tggtggatgt aatacccact gatggcatcg ttccgctcta
     9121 tatcaatcca caaggaatgg cgaaactgct gcgtaacgaa acgctgacca gtctgccgaa
     9181 gaatctcgaa ccggtttttt attacgccgc acaaacttta ttaatgccga agctggacgc
     9241 tttatctcaa caaccgcgtt atgtcatgaa gctggcccag atggaacccg gtgccgcctg
     9301 gcagtggcta cccataacct ggcaaccgct atgagacacg ggctgctggc gctgatttgc
     9361 tggctgtgtt gtgttgttgc ccatagcgaa atgctgaatg tcgaacaatc cgggctgttt
     9421 cgcgcgtggt ttgtgcgcat tgcacaggaa cagttccgcc aggggccaag cccacgctgg
     9481 tatcaacagg attgcgcggg cctggtgcga tttgcggcga acgaggcgct gaaagttcac
     9541 gacagtaaat ggctaaaaag taacggttta tctaaccagt atttgccgcc agagatgacg
     9601 ctaacacctg aacagcgtca actggcgcaa aactggaatc aggggaacgg gaaaaccggc
     9661 ccctatgtga ccgcgattaa tttgattcag tacaacagcc agtttattgg ccaggacata
     9721 aaccaggcac tgcctggcga tatgattttt ttcgatcagg gcgatgccca gcacttaatg
     9781 gtttggatgg ggcgttacat catctaccac accggaagcg ccacgaaaac tgataacgga
     9841 atgcgcgcag tcagtctgca acaacttatg acatggaagg acacccgatg gatacccaac
     9901 gattccaatc ccaatttcat tggcatttat cgtttaaatt ttctggcgcg atagcagcgt
     9961 gcttgtccct gtatcttgtg ggaacagggc ttactaatgc tgatgattcg cttccttcca
    10021 gtaactatgc gccgcctgcc gggggaacat tctttttgct cgctgacagc agttttagca
    10081 gcagtgaaga ggcgaaagtg cgactggaag cgccggggcg tgattatcgg cgctatcaga
    10141 tggaagagta cggcggtgtg gacgttcgcc tgtatcgtat tcctgacccg atggcatttt
    10201 tgcgccagca gaaaaacctg catcgcattg tggtgcaacc gcaatatctg ggcgacggac
    10261 tgaacaatac gctgacctgg ctgtgggata actggtacgg caaatctcgc cgcgtgatgc
    10321 agcgtacttt ctcttctcag tcacggcaga atgtgactca ggcgttaccc gaattacagc
    10381 tcggcaatgc cattattaaa ccttcccgtt atgtacagaa cagccagttt tccccgctga
    10441 aaaaatatcc actggtggaa cagttccgtt acccattatg gcaggctaca tcggtcgagc
    10501 cgcagcaagg ggtaaaactg gaaggcgcat ccagcaattt catttcgccg cagccgggca
    10561 acatctatat tcctctcggc aaacaagagc cgggactgta cctcgtcgag gcgatggtgg
    10621 gtgggtatcg ggcgacgacg gtggtgtttg tttccgatac cgtggcgctt agcaaagtgt
    10681 caggtaacga gcttctggtg tggaccgcgg ggaaaaaaca gggtgaagcg aagcccggct
    10741 cagagatctt gtggactgac ggtcttggcg tgatgacccg cggtgtgacc gatggcagtg
    10801 gtaccttgca gttacaacat atatcgccag aacgttcata tattctgggt aaggatgctg
    10861 aaggcggcgt ttttgtctcc gagaactttt tctacgaaag cgaaatctac aacacccgct
    10921 tgtatatctt taccgatcgc ccgctatatc gcgcaggcga tcgtgtcgat gttaaaatga
    10981 tgggccgcga gttccacgat ccgttgcatt catcccccat cgtcagcgca ccggcgaagc
    11041 tttcggtgct ggacgccaac ggcagtctgt tgcaaaccgt caatgtcacg ctggatgcgc
    11101 gtaatggcgg gcaggggagt ttccgcctgc cagaaaatgc cgtcgccgga ggttatgagt
    11161 tacgtcttgc ttaccgcaaa caggtctata gcagcagttt tcgcgtggca aactacatca
    11221 aaccccattt tgagattggt ttagctctcg ataaaaaaga gttcaaaact ggcgaagcgg
    11281 tcagcggcaa actgcaactg ctctacccgg atggcgagcc ggtaaaaaat gcccgcgtgc
    11341 agttaagttt gcgcgctcag caattatcaa tggttggtaa cgatttgcgt tatgcaggac
    11401 gtttcccggt gtcgctggaa ggcagtgaaa cggtttccga cgataatggt catgtggcgt
    11461 taaatctccc cgccgccgat aaaccaagcc gttatttgtt aactgtctcc gccagtgacg
    11521 gcgcggcgta tcgtgtcacc accaccaaag agatcctcat tgaacgtggt ctggcacatt
    11581 acactttgag caccgccgca caatacagca atagcggtga atcggttgtt ttccgttatg
    11641 ctgtgctgga atcttcaaaa caggttcctg ttacgtatga gtggttgcgt ctggaagacc
    11701 gcacgagcca cagcggagat ctaccttctg gcggcaaatc ctttaccgtc aatttcgata
    11761 aaccaggcaa ttacaacctg acgttacgcg ataaagacgg cttaattctc gccgggttaa
    11821 gccatgtcgt cagcggtaag ggcagcacgt cgcatactgg tacggtagat atcgtggcag
    11881 ataaaacact gtatcagcca ggcgaaaccg cgaagatgct gattaccttc ccggagccaa
    11941 ttgacgaagc attattgacg ctggaacgcg atcgcgtgga acagcagtcg ctgctatcgc
    12001 atccggcaaa ctggctaacg ctacaacgtt taaacgatac ccagtatgaa gcccgcgtac
    12061 cggtgagcaa ttcctttgcg cctaacatca ctttttcggt gctgtatacc cgtaacggtc
    12121 agtacagttt tcagaacgcc gggattaaag ttgccgttcc ccagctggat atccgggtga
    12181 aaacggacaa aacccattac cagcctggtg aactggtcaa tgtcgaatta acctcgtcgc
    12241 tgaaaggtaa acctgtttct gcgcagttaa cggtaggcgt ggtcgatgaa atgatctacg
    12301 cgctgcaacc ggaaatcgcg ccgaatatcg gcaaattttt ctatccgctg gggcgtaaca
    12361 atgtgcgtac cagctccagt ctgtcgttta tcagctacga tcaggcgctc tccagcgagc
    12421 cggttgcgcc tggcgcgact aaccgcagcg agcggcgagt aaaaatgctg gaacgtccac
    12481 ggcgtgaaga ggtggatacc gcggcctgga tgccgtcact cacaaccgat aaacaaggca
    12541 aagcgtattt cacgttcctg atgcctgatt cgttaacccg ctggcgcatc accgcgcgtg
    12601 ggatgaacgg cgacgggctg gtcgggcagg ggcgtgctta tctgcgttcg gaaaaaagtc
    12661 tctacatgaa atggagtatg ccaacggtgt atcgcatggg cgacaaaccg gcggcaggac
    12721 tgtttatctt cagtcagcag gataacgaac cggtggcgct ggtgactaaa tttgcaggtg
    12781 ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg ctgacgcaga
    12841 atattcagca atctggcttg ttaagtgcag aactgcaaca aaatgggcaa gtacaggaca
    12901 gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacaa cagaaaaatg
    12961 tcatgctcgg cggtggcgat aatgcgctga tgttgcccga gcaggcgagc aatatccggc
    13021 tacaaagtag cgaaacgccg caggagattt tccgtaacaa tcttgatgcg ttagtcgatg
    13081 aaccgtgggg tggcgtgatc aacaccggta gccgtctgat cccgctcagt ctcgcctggc
    13141 gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg attcaggata
    13201 accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg tggggtgaag
    13261 atggcaatgg cgacgccttc ctcacggcat gggcatggta cgccgactgg caggccagcc
    13321 aggcgctcgg cgtaacgcag caaccggaat actggcaaca tatgctcgac agttatgccg
    13381 agcaggcgga caacatgccg ttattgcatc gggcgctggt gctggcgtgg gcacaggaga
    13441 tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc cggcgcggaa
    13501 ctaaaactaa agatttctct gaggcagaca ccagcgatat caatgacagt ctgatcctcg
    13561 atacacctga atctccactg gcagatgcgg tggcaaacgt cttaaccatg acgttgctga
    13621 aaaaagcgca gttgaagtcc actgtgatgc cacaggttca gcaatatgcg tgggataaag
    13681 cggcaaacag caatcagccg ctggcgcaca cggttgtgct gctcaatagc gggggcgacg
    13741 ctacccaggc ggctgctatt ttaagtggtt tgaccgctga gcaatctact attgagcgcg
    13801 cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt gttttgcctg
    13861 cgcctgcggg cgcatgggcc aaacataagt taactggagg gggcgaatac tggcgttggg
    13921 ttggtcaggg cgtgccggac attctctctt ttggtgacga attatcgccg caaaatgtgc
    13981 aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg gtgaccgttg
    14041 aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact ctgcagccgg
    14101 tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg cttaccagcg
    14161 agcaggatgc cgttctgcgc tacggtcagg tggaagtacc gctcccgcca ggggctgacg
    14221 ttgagaggac tacgtggggc atttcggtca ataaacccaa cgccgcgaaa cagcaggggc
    14281 aattgctgga aaaagcgcgc aatgaaatgg gcgaactggc ctatatggtg ccggtgaaag
    14341 aactgacggg aacggtcact ttccgctatt tgctgcgctt ctcgcaaaaa gggcaattcg
    14401 ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc acagcaaagt gttgcggcag
    14461 ggagcgaatg gactgggatg aaggtgaaat aagtgaactg gcgcagaatc gtctggctat
    14521 tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg gcgctgcggg
    14581 gcgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa gtctcagcat
    14641 tacctgacac gctcaccacg ccgttaggga gtttatggaa actgtatgtt tacgcctggc
    14701 tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc ccggaagagg
    14761 tttattgttg tcaggctggg gagagtataa cgcgcgatac cgcgctggta cgttcctgtg
    14821 ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg caatactggc
    14881 aacagcgtca ggcacctgcg tggttagcgt ctcttactac gctgaagcca gaaacctcgg
    14941 taacggttaa atcactgctt gattcattag cgacgttacc cgcgcaaaac aaagctcagg
    15001 aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatct atgctcggta
    15061 gtcgggtacg ggtgaaaacc tggagttggt ttgccgatga tagatgataa acaggaaatc
    15121 cgtcagggtg gttttgctgg ctggttaacc gatggtacac cgttgtgggt tactggcagt
    15181 ggcaccagca aaacggtact gacccgttat gcgacggcgc ttaatcgggt tttgccagtg
    15241 ccgacgcaag ttgccagtgg gcaatgtgtg ctggtggatc tgtttgctcg ttatccgctg
    15301 aagaaagtta ctgaggaaaa gagtacgacg gcagtcaaac ccggcgtact taatgggcgc
    15361 tatcgtgtga cgtttgccaa tggtaaccac atgacatttg tcagccacgg tgagacaacg
    15421 ttactgacgg taaagggcaa actgaaatta cagtcgcatc tcgatcgtga agagtacgtt
    15481 gccagagttc tggatcgcga ggcgaaaagt acgcctactg aggcagcgaa agccatgacc
    15541 gtggcgattc gcacgtattt acagcaaaac gccgatcgcg atggtgattg cctgagcatc
    15601 ccggacagta gcgctaccca gcgcgtttca gcttcgccag ccactgtagg cgcgcggacg
    15661 ataaccgcct ggacgcagga tctcatctat gcgggcgatc cggttcatta tcatggcagc
    15721 cgggttaccg aagggacgct ttcctggcgg cacgctactg cccaggcggg gcagggagag
    15781 cgttatgatc agatacttgc gtttgcttat cccgacaaca gccttagccg ctggggagcg
    15841 ccacgctcaa cctgccagtt attacccaaa gcgaaagcct ggctggcgaa aaaaatgccg
    15901 cagtggcggc gtatattaca aggtgagacg ggatacaacg aaccagacgt gtttgcggtc
    15961 tgtcgtttag tctcaggttt cccctatacc gatcggcagc agaaacggct gtttattcgt
    16021 aatttcttta cgcttcagga tcggctggat ttaacccatg agtatctgca cctggccttt
    16081 gatggttatc ccaccgggct tgatgagaac tatatcgaaa cgctgacccg acaattatta
    16141 atggactaat gctatgcgaa agatttttct tccgttgtta ctggtggcgc tttcgcctgt
    16201 cgctcacagt gaaggtgtgc aggaagtcga gattgatgca ccgctctctg gctggcatcc
    16261 ggcagagggc gaagatgcca gtttttcaca aacgatcaat tacccggcgt cgtccgtcaa
    16321 catggcagat gatcaaaata tctcagcgca gatccgcggc aaaataaaga attatgctgc
    16381 ggcgggtaaa gttcagcagg gccggctggt ggtcaacggt gccagtatgc cgcagagaat
    16441 tgaatcagat ggttcttttt cacgccctta tattttcact gaaggcagca acagcgtgca
    16501 ggtcatcagc ccagatgggc aaagccgaca aaaaatgcag ttttactcaa cgccgggtgc
    16561 tggagcaatt cgcgcgcgtt tacggctggt tctctcgtgg gatacggaca ataccgacct
    16621 cgatcttcat gtcgttacgc ctgacggtga acatgcctgg tacggtaaca ccgtgctgaa
    16681 aaacagtggc gcactggata tggatgtcac gacagggtac ggacccgaga ttttcgccat
    16741 gccagcgcca gttcacgggc gctatcaggt gtatatcaac tactatggcg gacgcagcga
    16801 aacggaatta accaccgccc aactgacgct gatcaccgat gaaggatcgg tcaatgagaa
    16861 acaggaaaca tttattgtac cgatgcgtaa tgctggcgag ctgacgctgg tgaaaagttt
    16921 tgactggtga ttaatggggt taccttcgcc gtcgatgttc aagaaaacgc ccgataactt
    16981 tcgctatcgg gcgtttttat tggttaattc aaccgctcaa tcaccatcgc aattccctgg
    17041 ccgccaccaa tgcacagtgt tgccagcccc agcgttttat cgcgtgcctg cattgcatgt
    17101 aatagtgtga ccagaatacg agcaccactg gcaccgatag gatgccccag cgcgatggcc
    17161 ccgccgttga cattcacttt ctcaggatca aagcccaggg ttttcccaac ggcaaggaac
    17221 tgtgcagcaa atgcttcatt agcctcaatg agatcaatat ccgccagttg cagccccgcc
    17281 agttgtaacg ctttttgcgt ggcaggtact ggccccatac ccatcaatgc ggggggcacg
    17341 ccaccgctgg cataactttt aatgcgtgcc aggggattca ggcctgctgc cagcgccgca
    17401 gattcttcca taatcaccag agcggcagca ccgtcgttaa tacctgacgc gttcccagcg
    17461 gtgactgttc ctgctttatc gaaggccggg cgcaatgcgc ctaacgcttc agccgtagaa
    17521 tccgctttcg ggaattcgtc ttgactgaag acgaaggttt tcttccgagt gacaacattt
    17581 accgggacga tttcggctgt aaaagcaccg gactcaattg cggctgccgc tttacgctgt
    17641 gaatgtagcg ccagttcatc ctgcatttca cgggtaattc cgtactcttt agccacgttt
    17701 tcggcggtaa tccccatatg ataaccatgg gtggcgcaca tcaggccatc gcgcaggatt
    17761 acgtcataaa cctgtccgtc tccaagacga taaccagagc gtgcttttgc atcgagtaag
    17821 tagggcgcta aactcatatt ttccataccc cccgccacaa tgctctgtgc ctgacctgcc
    17881 tgaatcgcct gtgcagcaag cgccacgctt ttcagacctg aaccgcacac tttgttgacc
    17941 gtgaatccgc acaccgtttc agccagtccg ctttttagca gcgcctgacg tgccggattt
    18001 tgccccagtc cggcttgcaa cacgttaccc ataatcactt catcaacgtg tagtgaatcg
    18061 atttttgcac gttcaatggc ggctttaatt actgtcgccc ccaggtcgat ggcgctggtg
    18121 gaagcgagtg aaccgttaaa actaccgata gcagtacgta ccgcactgac gatgacacaa
    18181 tttttcattt tatattcctt ctgtctgtag ggttgccgtc agaacagcgt taaaccaatg
    18241 acgaaaatga cgccggagaa gagcagggca gtgatgcaat agcccatgat gtcgcggaca
    18301 ccgagtccgg cgattgccag tgctggcagc gcccagaacg gttgtgccat gttcatccat
    18361 tgctcgccgt aggcgatcgc cattaccgat ttaccgagat cagcgcccag cgcctgggct
    18421 gcgggcatca cgaacggccc ctgaataacc cagtgcccgc cgccagacgg aacggcgaag
    18481 ttaatcagtg cagaactaaa aaaggtcatg accgggaagg tgtctttgtt cgcaacattg
    18541 atgaagaatt cggtaatgag tccgcccaga ccggagtgct ccatcatcag ttggatcccc
    18601 gcgtagaagg ggaattgcac cagaataccg gcggtactgc gtgctgccgc gctgatagca
    18661 cgcatataag ccattggcgt tttatgtagc agcagacccg caatcataaa catcaggttg
    18721 acggtattga tggtgatgtt gaagccatgt tcgctgaagt acatcgccag gtaagcgata
    18781 ccgagtgcgc cgatgatcaa cgtcagaata cggctttctt ccagtcgctc ggacggtgga
    18841 gcatctttcg gtagttgctt ttgaaaatca gcctcttcca tgagtagttt cggatcgata
    18901 ctcaccacgt cagacggttt tggcatcatc atgcgggtga taaatggcat caccacaatc
    18961 aacgccacag tgatgaaaat attaaaacca ctgaacagag tatcgcccac cgggatcagc
    19021 ccggcgatat gctcaaccgg gttgcccggt gttgcagcca acagaggcat tgagccagag
    19081 aaaccgccac cccaggtgag aaaaccaatg taggcgcagg caatgagcaa cggataatca
    19141 gaaccgggta ctcgccgggc gacttcacgg gcgaacattg caccgacaac caaaccaaat
    19201 ccccagttga tgacacaagc gactgaaccg aagaaagtaa ccagcatgac gccctgtacg
    19261 ggcgtctttg cggcggaggc ggcagtacgc agcaaacttt tcaccggagc ggagctggca
    19321 agggcatgac cggtaacgat gataagcgcc atctgcatac caaacgccag caagttccag
    19381 aaaccgtcac cccacatttt caccatgctg atcggcgttt gtggtgttaa ccaaagcgcg
    19441 atcacgaatg ttagcaatgt cagcaacatg gcaaagatca gtggatcggg aagccaccgg
    19501 ctgacaaaac gcgtcataaa acgcgatatg cgaccaatca taaatcaccc cgttgcgtat
    19561 tcagatcggc agctacttca aaccgagctt ctgttttggc acgcacggtg gctaaatcac
    19621 acccgtcggc aatttcggtg agccacattt tgccgtcaat aaaacgaaag acagccagtt
    19681 cagtaaccag catatgcacc gcatgttgcg cagtgagtgg catggtgcag cggcgcaaaa
    19741 tttttgctga accatctttg gcgcaatgtt ccatggcgat gatcactttg cgcgacccgg
    19801 tcaccagatc catcgcgccc cccataccgg gcaccatttt cccaggcact acccagttcg
    19861 cgaggtttgc ttcttcgtct acttgcaaac caccgagcac gcaggcatca atatgaccgc
    19921 cacggattag cgcaaatgac atggcgctat caaacatggc tgcgccgggt aaaatgccgc
    19981 acggttgtcc gccagcgttc accagatctg gatgcgctgt cgtgaccggg cctaaaccga
    20041 ggaagccgtt ttccgattgc agagtgatat gaataccctc cggtaaataa ttggcgacca
    20101 ttgtgggtaa accgatccct aagttaacga tgtcaccatc acgaagctct tgcgccacac
    20161 ggcgcgcaat acgttgtttc gcatccatta tttgctctcc tgtgaaacga tgatgtggtc
    20221 gataacggca ccaggggtga caatatggtc aggttgcagc tcgccggttt cgaccagttc
    20281 atccggctct accagcgtga tatcagccgc aagggctatc agggggttaa agttgcgggc
    20341 gctaaggtga taggtcaggt tgccaagtgt gtcgcaacga tgagcgcgaa ttagcgccag
    20401 gtcggcgcgc agcgggcgtt cgagcagcca ggttttaccg tcgagtgtca gtgtctgttt
    20461 gccttcctct acgacggtgc cgacaccagt tggggtgaga aaaccaccaa gtccagctcc
    20521 accacagcga atttgctcga ttagcgtacc ttgcggcacc agaacgacgt ccatctcacc
    20581 agatatcatg cgccgacctg tttccgggtt ggtgccgata tgtgaagcaa tcactttgcg
    20641 gactcgacca ttgacgatga gcggaccgat gccggtatca acaaacgcgg tatcattggc
    20701 tatcaatgtc aggtcgcgaa caccagattc cagtaatgct tcaaccaggc gggatggagt
    20761 gccaatcccc ataaatccgc ccaccatgat ggtcatgccg tcacgaaaga agccggtggc
    20821 gtcttgtaat gtcatcaatt ttgttttcat tttttatccc tcttgcatac accgttgtgg
    20881 gtacagtcag ggaatagcaa ggggagtgcc agaatgagat tattgaatgt tgttatttaa
    20941 ctaattgaat agaaaaggat aaattagatg gggattcata aggagaatga gcggctatgc
    21001 agaaaattgc gcactgtgca aatttctgca tagcaagttt tggcgttata catccgccgg
    21061 atcgataccg tattcctgga gtttatacat caatgcacgg cgactgatgc ccagcattaa
    21121 agcagtgcgg gttcggtttc cttcttgttg ttccagcact tccataatga tgcgtttttc
    21181 gacgcgttta atttcctctt ttaaattacg ctcaccgaca ggggctgttt ttgcttcacc
    21241 agcattacag actggctgac gaatctgcgg cggaagatcc tcagaaaaaa tgatcgggcc
    21301 tgaattcatc acgacggcgc gttcaataac gttggaaagc tctcgaatat ttcccggcca
    21361 tgaccaggcg gtaagcagtg acattgccat cggatcgatg tcgataatat cgcgctgatt
    21421 ctcactactg aatttttgca aaaagtgatt agctaacagg gaaatatctt cccggcgatc
    21481 gcgcagaggc ggcagtatta aatgaataac gttaaggcga taaaagagat cttcacggaa
    21541 ggtgccttct ttcaccattg cctgcaagtc gcggttggtg gcagcaatga tgcggatatc
    21601 aacttttatg gtctgatggc cgccaatccg ttcaaattcc cgttcctgca gaatgcgtag
    21661 taatttggct tgcagtacca gcggcatttc gccaatttcg tcgaggagca gagtaccttc
    21721 gttggcacgt tcaaataatc cctgacgcaa ggtttgtgca ccagtaaatg cacctttttc
    21781 atgaccaaac agttcacttt cgagcaacga ttccggcagc gccgcgcagt tgactttaat
    21841 gaacggcccc tttgcccgcc gcgaattgta gtgaatcgct ctggcaatca actctttccc
    21901 ggtgccgctt tcaccgctaa tcaagacgct ggcctgagaa agggcaattt tggcggtgtc
    21961 tttgcagatg tccatcatcg ccgggctgtt ggtgagaatg tgcccccatt gccagctggt
    22021 gctcagtgcc tggtgcagat gacggatctc ttttttcatt gactggagtt gtaaagcgcg
    22081 ctgaacgatt aaattcaact catcgagatc aaacggtttg ataacatagt cgaaggctcc
    22141 gcagcgtagt gcttcgacgg cggtttccac ttcagcatag gccgtcatca gaataacggg
    22201 tgtccgggtc tcatggctgc gcatctcctt tagtgccttg atgccgtcca tctctggcat
    22261 gcggatatcc atcaacacca cgtcagggtg aatatcggca aacaggtgta atgctgtgcg
    22321 cccgttgttc gcacaatgtg tttcgaatcc ttgtagtgca aaagcggtgc tcagcatacg
    22381 gcgaacatta tcttcatcat ccacaataag gatgcgatta atagcagtca tacagtctga
    22441 tttccctgcg ggttgatcgg taaaataagc gtgaaggttg cgccgtagcc cggcaaactg
    22501 gcgacgcgaa tatcaccctg atgggcatta atgatgcgtt gacttaacgc cagaccaagc
    22561 ccggttcctg aggctttggt agtgaaaaag ggatcgaaga tctttttttg cagcgagaga
    22621 tcaatgccac tgccgttgtc ctctatcgaa atggcctgtt gcgagtcgct atactgccag
    22681 gttcgaatgc gaattttccc tcgtgcactg atagcctgga cggcattgat caggatattc
    22741 agtagtacct gtttgagcag ttcacgatcg gcgttaatcg ggcttaattc attatccagt
    22801 tcgcttatga agtcgacccg cgcttgtacg ccggcggttt gtaccagtac cagagtttct
    22861 tcaaccaatg cattgaggct gacttgttgc cattgactgt ggcgtggacg tgaaaattcg
    22921 agcaattgct gaataacttt gttaattgaa tcgatttctt tgagtactac ggacagatat
    22981 tcctgatgta ttgggtcacg ggtttgttgg cgcaagatct gtacataacc acgaatagct
    23041 gttagcggat tacgtacttc atgcgccacg ccagccatca gctcacccag tgtggcgagg
    23101 cgttctgctt gcgccatgcg gcgctgggtt tctttgcggg cagttaaatc agagaaaatc
    23161 accaaagcac ctatcatttc accgtgcgtg ttatgaatac gactggtcgt gacactgagt
    23221 tcaatggtgc ggtcacggcc tggaaaactg atctccagtg ctacatgttc ggtgccatgt
    23281 tccagcgtat ccagtactgg actgtagaac tgagtattgt cgaacaacat ggagtaaggc
    23341 tgccctacca gttcatgacg ttgatagcca gtgatgactt ctgctgctgg gttcatggtg
    23401 gttacatcac cctggcggtc aatggcaatg acgccatcgg cagcgttttc aataatcaga
    23461 tcgttaagtg ttcgcgtttc acgcagtgcc tgggcgaggt tgttaacact ctgactgatt
    23521 tgccccattt caccgggcaa ttgtggtaat cgagtgggaa tattttgtgc cagagtcgag
    23581 aggccatcgg tgatgatatc tatattggcg ctcaggcgac gggagaaaag gacaatcaac
    23641 agcaggctta tcagcaaacc ggcggtgagc acaatgataa tcctcacatc cattttccag
    23701 gcctggcggc gaatatcttc ggttaattca ttggcccaga tatagccgag gatttcacca
    23761 ttacgctcaa tgggaatcat tgaattcaaa atatcgccgc gcacctgcct gcctgaataa
    23821 accaaagggg tatttgtacg catgacttcg cgaccagggt gatctgcggc aatggtaacg
    23881 cccacattat tctgatatag cgctgaaggc gcgtaggtga ttatcgcatc cagcgttttg
    23941 ttgtaataac cagcgccgat gccagggaag gcgtgagtga tattttcggt aatgggggca
    24001 agttctgcat ttaatgcgcg gatacgctcc tcacgcggta agtcgatgta gagatcgtag
    24061 cgattgccta gcgcctgatt aagcaggttg accacggcag aaagtttttt ctctttttca
    24121 gataagactg ctgaacgtcc ttccgtttct acgatataac caatagtaag cgttgggaca
    24181 atgaccatca ggattgccat caggatcatt tgattgcgta agcggcgtgg ataaatccac
    24241 ttcatataat gcatgctgaa atccactagt cttgtccggt atatgacgat tatcagaggt
    24301 taaggtgatg atttctcggc ggtgtatcat attccagaga agagagaaca ttgcggtaac
    24361 acgcttttac cgctacctta accacactcc atcggtcacc tgaggcggag cttcgcccct
    24421 ttgaaatacc ttgcttcttt tcgtacaacc ctgaaagcct cgcgctacat gttcagagca
    24481 ttggcgttag tgctctggct gttgattgct ttttcatccg ttttttacat cgttaatgcg
    24541 ttacatcagc gagaatcgga aattcgtcag gaatttaatc tgagttccga tcaggctcag
    24601 cgctttattc aacgcacctc tgatgtgatg aaagagctga agtacatagc tgaaaaccgc
    24661 ttatcggcag aaaacggtgt gctttccccg cgtggacgag aaacgcagac ggatgtgcct
    24721 gcgtttgaac cgctgtttgc tgactctgat tgttccgcaa tgagtaacac ctggcgaggt
    24781 tctctggagt cattggcgtg gtttatgcgc tactggcgcg ataatttttc tgcggcttac
    24841 gatctaaacc gggtattttt aatcggcagc gataacctct gcatggccaa tttcggtctg
    24901 cgtgatatgc cagtggaacg cgataccgcg ttaaaagctt tgcatgaacg cattaataaa
    24961 tatcgaaatg caccacaaga tgatagtggc agtaacctct actggatcag cgaaggtcca
    25021 cgccctggcg tcgggtattt ttacgccttg acgccagttt atctggcgaa ccggttgcag
    25081 gcgcttttgg gtgtcgagca aaccatccgg atggagaact ttttcttacc tggtacgttg
    25141 ccgatggggg ttaccattct tgatgaaaat ggtcatatcc tgatttcgct taccggacca
    25201 gaaagcaaaa ttaagggcga tcctcgctgg atgcaggaac gctcctggtt tggctatacg
    25261 gaagggttcc gggagctggt gctgaagaaa aatctgccac cctcatcgct aagcatcgtt
    25321 tattcagtgc cagttgataa agtgctggaa cgcattcgca tgttgatcct taacgcaatt
    25381 ttgctgaatg tgcttgccgg agctgcattg tttactctcg cgcggatgta cgagcggcgt
    25441 attttcattc cggcggaaag cgacgccctg cgactggaag aacatgagca gtttaatcgc
    25501 aagattgtcg cctccgcgcc agtgggtatc tgcattttgc gtaccgctga tggcgtcaat
    25561 attttaagta acgaactggc gcatacctat ctcaatatgc ttacgcatga ggaccgccaa
    25621 cggctgacac aaattatctg tgggcagcag gtcaattttg ttgatgtcct gaccagcaac
    25681 aataccaatc tgcaaatcag cttcgtccat tcgcgctatc gtaatgaaaa cgtggccatt
    25741 tgtgtgctgg tggatgtttc ttcgcgcgtg aagatggaag agtcgttgca ggagatggca
    25801 caagcagcgg aacaggcgag ccagtcaaaa tcgatgttcc tggccaccgt cagtcatgag
    25861 ctgcgaacgc cgctgtatgg cattatcggt aacctggatc tgttgcaaac caaagagtta
    25921 ccgaaaggcg tcgatcgtct ggtgacggca atgaacaact cttccagcct gttgttgaaa
    25981 attatcagcg atattctcga tttctcgaaa attgaatcag aacagttgaa gatcgaaccg
    26041 cgtgagtttt caccgcgtga agtgatgaac cacatcaccg ccaactattt accgctggtg
    26101 gtacgcaagc agttaggctt gtactgcttt attgaaccgg atgtgccagt ggccttaaat
    26161 ggcgacccga tgcgtttaca gcaggtcatc tccaacctgt tgagtaacgc cataaaattc
    26221 accgataccg gctgtatagt tttgcatgtt cgcgctgatg gcgattatct ctctatccgt
    26281 gttcgcgata ccggcgtggg gataccggcg aaagaagtgg tgcgtttgtt tgatcccttc
    26341 ttccaggtcg gaacgggcgt acagcgtaac ttccagggga ctggtctggg tctggcgatt
    26401 tgtgaaaaac tgatcagcat gatggacggc gacatctcgg tagattcaga accgggaatg
    26461 ggcagccagt ttaccgtgcg tattccgttg tacggcgctc agtacccgca gaaaaaaggt
    26521 gtggaagggt tgagtggtaa acgctgctgg ctggcggtcc gcaatgcgtc gctctgtcag
    26581 ttcctggaaa ccagtttgca gcgcagcggc atcgtcgtta caacatacga agggcaggaa
    26641 ccgactcccg aagatgtgtt aattactgac gaggtagtga acaaaaaatg gcagggcaga
    26701 gcggtagtga ccttctgtcg tcgccatatt ggtattccgc tggagaaagc gccaggggag
    26761 tgggtacaca gtgtggctgc tccgcatgag ctaccggcat tgttggcgcg tatttatttg
    26821 atcgagatgg agagcgacga tcctgctaac gctctgccgt cgacggacaa agcggtcagc
    26881 gataatgacg atatgatgat tctggtcgtg gatgatcatc cgattaaccg gcgtctgctg
    26941 gcagatcagt tgggatcgtt aggctatcaa tgtaaaaccg cgaatgatgg cgtcgatgcg
    27001 cttaatgtac ttaacaagaa tcatattgat atcgtgctta gcgacgtcaa tatgccaaat
    27061 atggatggtt accgcttgac gcaacgcatt cgtcagttgg gactgacgtt gccggtaatc
    27121 ggagtaactg ctaatgcgtt ggctgaagag aagcagcggt gtctggagtc cggtatggac
    27181 agttgcctgt cgaagccggt aacgctggat gtgataaaac agacgctgac ggtatatgcc
    27241 gagagggtca ggaaatcgcg ggaatcgtag gacggataag gcgtttacgc cgcatccggc
    27301 attcagtgca aatgccagat gcgacgctga cgcgtcttat ctggcctact ttaatgtaat
    27361 ctgttgaaat aatgggaatc gtaggacgga taaggcgttt acgccgcatc cggcaatccg
    27421 tgcaaatgcc agataagaca ctaacgcgtc ttatctggcc tacaggtgat tagtctttat
    27481 ctgccggact taacgtcaca gaagagagat aattcagcag agcaatatcg ttctcgacac
    27541 ccagcttcat catcgcagat ttcttctggc tactgatggt tttaatactg cggttcagct
    27601 ttttagcgat ctcggtcacc aggaagcctt ccgcaaacag gcgcagaact tcactctctt
    27661 ttggcgagag acgcttgtca ccataaccac cagcactgat tttttccaac aggcgagaaa
    27721 cgctttccgg ggtaaatttc ttccctttct gcagcgcggc gagagctttc ggcagatcgg
    27781 tcggtgcacc ttgtttcagc acgatccctt cgatatccag atccaatacc gcactaagaa
    27841 tcgccgggtt gttgttcatg gtcagaacaa tgatcgacag gcttgggaaa tggcgcttga
    27901 tgtacttgat taaggtaatg ccatcgccgt acttatcgcc aggcatggag agatcggtaa
    27961 tcaacacatg tgcatccagt ttcggcaagt tgttgatcag tgctgtagag tcttcaaatt
    28021 cgccgacaac attcacccac tcaatttgct caagtgattt gcgaataccg aacaagacta
    28081 tcggatggtc atcggcaata attacgttca tattgttcat gtaataggct accttgctac
    28141 agcaagctct tgacataact gtcaatgtcg ctgatgtatt tttctattcc tggaacatcc
    28201 ttctcacgaa tcagatgttc cagcgtttca cataactgct tgccgggtac cagatttagc
    28261 atggcaaata cgcctttaag acgatgagcc gtttgggcta acgcagcaaa gtcactggtt
    28321 gctgcttcag tatatagcct cttaacatca tccggtactg tgtctacaaa gagcgcataa
    28381 tagccgctgg catggagttg cgcattttca tctccgccca gaggcgattc tgtcacctct
    28441 tcctgcgcca gttgcacttc aattaatggt aagaccgctt cctgcatagc gttgctcata
    28501 ttgaaattga cgcacaattg accaggccca atttcccgta cgccagactc atcatcgctt
    28561 aaaagcaagc cagaggcagt aagattagac ggattatccg ttaaaaagat atcataatct
    28621 tgactaatta atctttcatc gggtgtgata caggttgcac cccaattttc taactggcga
    28681 gtgacaatat tccgaatttc tgccgaagta acatccacca ttacgcagac atcatccagt
    28741 aatcgctctt cttcctcttc aacttccggg tcagctgcga gcattttgat atgcacagag
    28801 tagcgtgtac caagcccatc ccgcgttttg atgtttaaat gaccgcccag tttacgcgcc
    28861 agttgatcgc tcagccagaa tgccagcggg tccgccttgc catagcgatc gttttgggtc
    28921 tggttgataa acgggaagtg caaattatcc atttcatgaa tgcttacgcc ttctccagtg
    28981 tccagaatgc ggaacgtcag gcggtcttcg gaggactcat cctgatcaac ctcaagggtg
    29041 atttttccca attgcgttga ggtcacggca tattgcatca gtagcagcaa aatacgtcgt
    29101 aaggcatcgc gatcgccgcg gcgcatatcg tgtgctttca gatgattgtt aatcagcagt
    29161 tgcagacctt tacgcttgat ggcaggcaac actgaaggca caacttcatc aattaaatcc
    29221 tgcacggaga acagctccgt ctcacttttc cagctatcgt ccgcaagcat gttcgctaac
    29281 tgtatttcat cgaccagccg caccagcaca tctgcctgat tcgccagttg tttgccttcc
    29341 ggggcgttga gtttagccgc gctctccgcc agggactgtg cgggttcttt cagcgcatcg
    29401 ccaatgtttt tcataaaggt catccgcccc tgctggtttt tctcatacag acgctgcgcc
    29461 tgcttgagtt tcttgtttac cagcacttcg cgatcctgat cgcgaataat gaaaatttgt
    29521 gtgcgcggcg caacctggct gcgaaacatg cggatctcat acagctcgtt attgatcgtc
    29581 gcctgaataa tcccctgatg ctgttccgcc atggtggtga tgttttgcag attcaaatgc
    29641 ggcagcaaat gatcggcaat tttgttactt atgactgtgc ggttcgattc ctgatcgtga
    29701 accagcaggc cgagcggcag cagtgagact atctcttcat taattgcccg taaaatgcgc
    29761 aattcgttat tgaccgccgt gctggggacg ctttctgtac tgcggctgga gaaatggcgg
    29821 aatgtggtat agccaaataa cgccagcgcc agcaaaccga tgttcagcag cagtggcagc
    29881 agaatgtttt gcaacgtatc cagcagtaag gtgccgtaag gaacctgcca gaccaggcgc
    29941 atatcggtag agttgagtgc cgaggagatt tcaatcttcg tactgttaaa gtggatactg
    30001 acgctatccg tcccttcttt ctcattttca ttgtttcccg ttgccgtcgc gtctggctca
    30061 aggcggaaac tgtccagcgg catacccggt gggatcaaat cattaatcgg cagatcaaaa
    30121 gccacgaccg ttgccagatg tcccggctgg ttgaacgtgg tacgcaaggt aaagtaatga
    30181 ccgttctgcc aggccaggcg gcgcatgtta gaaaaacttt cgcgttcatc gagggcgttg
    30241 gcctgttgca acatctctgc acgacgtgaa tcaacaatgt cgctgacggt cgattcttta
    30301 aatccggagg tgagatcttt gaggggcagg gttgagatca gcaccagact gttatcctga
    30361 ccattcaggt aatacatcga ccacggtaca ttttctgcgc cccacaatgt gtccagatag
    30421 gtggacatcc gttgagtcat ctcaagcgtt gagttgtcgt gagagccaaa gattaacgct
    30481 tccgttttgc ggcgtggttt ctccagatag tagacatcct gtttcaggcg cgtctcttgt
    30541 aaaccttcgc cggaggaggg ggaggtcgtc gcggcaatgt tgtcgtagat ctgccaggtc
    30601 acgtaacgcc aggtatcgac gcgcttttgg attgcgtggg taatgtcgac aatctggtaa
    30661 cttttatctt tcagccaggc gttaacggcg ctttgtacca ttacacccat cgtcaccagt
    30721 aacacaatga tcaacagtaa aaagaagcgg gtaatgctcc ccggtaggag tgaaaagcgg
    30781 gtcgtggccg ttgtctcttt ctgacgcatt cgtgtttatg acctgttaaa acttcgcgaa
    30841 gtgatagggc agtataaagg gtacagtgtg aaattccaga ctcttacctg attgatgatt
    30901 ctctattctc cagtgatatt tatcatcctg cattgggtga aatagatgta caagcgccat
    30961 tttgctgtac aaataaccta caaaaagccc aacgaaatta attgcaaata aacgaagatt
    31021 gctggaaatt atgtctatgt taattatttg tgaaatagtt aacaagcgtt atagtttttc
    31081 tgtggtagca cagaataatg aaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg
    31141 catccggttg aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt
    31201 taatgatgat agcggaagat attctagttg cgagtgaagg ttt
//
LOCUS       DADSZD010000036        30994 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.036, whole genome shotgun sequence.
ACCESSION   DADSZD010000036 DADSZD010000000
VERSION     DADSZD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.036"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            10..1053
                     /locus_tag="J1Z28_001504"
     CDS             10..1053
                     /locus_tag="J1Z28_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBA7716348.1"
                     /translation="MQGIKIYTILASSLISGSIFAQTEISTANSSTVNASYVEPSAEK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSSFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEMAIRLVGSYY"
     gene            complement(1196..2560)
                     /locus_tag="J1Z28_001505"
     CDS             complement(1196..2560)
                     /locus_tag="J1Z28_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBA7716349.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(2649..3425)
                     /locus_tag="J1Z28_001506"
     CDS             complement(2649..3425)
                     /locus_tag="J1Z28_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBA7716350.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            complement(3430..4068)
                     /locus_tag="J1Z28_001507"
     CDS             complement(3430..4068)
                     /locus_tag="J1Z28_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBA7716351.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVTADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSNNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            complement(4065..5327)
                     /locus_tag="J1Z28_001508"
     CDS             complement(4065..5327)
                     /locus_tag="J1Z28_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBA7716352.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVES
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            complement(5324..6232)
                     /locus_tag="J1Z28_001509"
     CDS             complement(5324..6232)
                     /locus_tag="J1Z28_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7716353.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNTCAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            6398..7195
                     /locus_tag="J1Z28_001510"
     CDS             6398..7195
                     /locus_tag="J1Z28_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7716354.1"
                     /translation="MSAGQRKEQKLIPVERRQIILEMVAEKGIVSIAELTDRMNVSHM
                     TIRRDLQKLEQQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQP
                     GRCIYLDAGTTTLAIAQHLTHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENR
                     SCVGEAAATMLRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDA
                     TKYGQVATWLALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            complement(7246..7902)
                     /gene="pphB"
                     /locus_tag="J1Z28_001511"
     CDS             complement(7246..7902)
                     /gene="pphB"
                     /locus_tag="J1Z28_001511"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HBA7716355.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDHVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(8008..10569)
                     /gene="mutS"
                     /locus_tag="J1Z28_001512"
     CDS             complement(8008..10569)
                     /gene="mutS"
                     /locus_tag="J1Z28_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBA7716356.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLANLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            10854..11198
                     /locus_tag="J1Z28_001513"
     CDS             10854..11198
                     /locus_tag="J1Z28_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBA7716357.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            complement(11237..11665)
                     /locus_tag="J1Z28_001514"
     CDS             complement(11237..11665)
                     /locus_tag="J1Z28_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBA7716358.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(11710..13788)
                     /gene="flhA"
                     /locus_tag="J1Z28_001515"
     CDS             complement(11710..13788)
                     /gene="flhA"
                     /locus_tag="J1Z28_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBA7716359.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLSLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(13862..14872)
                     /gene="hypE"
                     /locus_tag="J1Z28_001516"
     CDS             complement(13862..14872)
                     /gene="hypE"
                     /locus_tag="J1Z28_001516"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBA7716360.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFSNPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(14869..15990)
                     /gene="hypD"
                     /locus_tag="J1Z28_001517"
     CDS             complement(14869..15990)
                     /gene="hypD"
                     /locus_tag="J1Z28_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBA7716361.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAETHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(15990..16262)
                     /gene="hypC"
                     /locus_tag="J1Z28_001518"
     CDS             complement(15990..16262)
                     /gene="hypC"
                     /locus_tag="J1Z28_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBA7716362.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(16253..17125)
                     /gene="hypB"
                     /locus_tag="J1Z28_001519"
     CDS             complement(16253..17125)
                     /gene="hypB"
                     /locus_tag="J1Z28_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBA7716363.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(17129..17479)
                     /gene="hypA"
                     /locus_tag="J1Z28_001520"
     CDS             complement(17129..17479)
                     /gene="hypA"
                     /locus_tag="J1Z28_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBA7716364.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPLCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            17692..18153
                     /gene="hycA"
                     /locus_tag="J1Z28_001521"
     CDS             17692..18153
                     /gene="hycA"
                     /locus_tag="J1Z28_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBA7716365.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            18278..18889
                     /locus_tag="J1Z28_001522"
     CDS             18278..18889
                     /locus_tag="J1Z28_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7716366.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            18886..20712
                     /gene="hycC"
                     /locus_tag="J1Z28_001523"
     CDS             18886..20712
                     /gene="hycC"
                     /locus_tag="J1Z28_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBA7716367.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLINHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            20715..21638
                     /gene="hycD"
                     /locus_tag="J1Z28_001524"
     CDS             20715..21638
                     /gene="hycD"
                     /locus_tag="J1Z28_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBA7716368.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            21656..23365
                     /locus_tag="J1Z28_001525"
     CDS             21656..23365
                     /locus_tag="J1Z28_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HBA7716369.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWITVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            23375..23917
                     /gene="hycF"
                     /locus_tag="J1Z28_001526"
     CDS             23375..23917
                     /gene="hycF"
                     /locus_tag="J1Z28_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBA7716370.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            23917..24684
                     /gene="hycG"
                     /locus_tag="J1Z28_001527"
     CDS             23917..24684
                     /gene="hycG"
                     /locus_tag="J1Z28_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBA7716371.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            24681..25091
                     /locus_tag="J1Z28_001528"
     CDS             24681..25091
                     /locus_tag="J1Z28_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HycH family protein"
                     /protein_id="HBA7716372.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDDYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            25084..25554
                     /gene="hycI"
                     /locus_tag="J1Z28_001529"
     CDS             25084..25554
                     /gene="hycI"
                     /locus_tag="J1Z28_001529"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBA7716373.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            25597..26352
                     /locus_tag="J1Z28_001530"
     CDS             25597..26352
                     /locus_tag="J1Z28_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716374.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDGEYAQKRVLKPLLTMPYDA
                     EQTWHFRKVSVAGVTLEKMPNEEDYWRLTAKDRAGKSWFVPVGVLQNMAGNAQFYRAD
                     LDRNGIQDLVIWRGVSGNGLAPSSFLILMTFNQQGRPCVFQVDGFYTANETGVDDLLD
                     LQGNGHTQLLDMQFDSGYWITSLYQVKEAKWQRVHGWFGKLSYPALTRFTYTSNRKLV
                     IKPVAGRNPQTKDLALTQRCLIKGDVLDGVNQN"
     gene            26515..26826
                     /locus_tag="J1Z28_001531"
     CDS             26515..26826
                     /locus_tag="J1Z28_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7716375.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            26823..27242
                     /locus_tag="J1Z28_001532"
     CDS             26823..27242
                     /locus_tag="J1Z28_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7716376.1"
                     /translation="MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(27334..27762)
                     /locus_tag="J1Z28_001533"
     CDS             complement(27334..27762)
                     /locus_tag="J1Z28_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716377.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNNVIGLVELIIQKAAGEKITSSKQIDQLDSYRLTSIFSKKLQGR
                     LQTIDDTHEWIMLFEGRAREKAKAYLIEITDKLRVVKTTA"
     gene            28147..28674
                     /gene="hydN"
                     /locus_tag="J1Z28_001534"
     CDS             28147..28674
                     /gene="hydN"
                     /locus_tag="J1Z28_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBA7716378.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            28827..>30994
                     /gene="hypF"
                     /locus_tag="J1Z28_001535"
     CDS             28827..>30994
                     /gene="hypF"
                     /locus_tag="J1Z28_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBA7716379.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEEPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVCESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGLAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQS"
BASE COUNT         7153 a         8012 c         8277 g         7552 t
ORIGIN      
        1 ggactacgta tgcaaggcat taaaatatat acaattctag cctcttctct tatatctggt
       61 agtatttttg ctcaaacgga aatttcgact gcaaattcgt ctacagttaa tgcaagttat
      121 gttgaacctt cagcggaaaa aataatatcg ccaagcgata aactcaataa cctttttgaa
      181 agaaatatgt ctcagcctta tattttacaa aaaattggcg agagaactta ttatgttcaa
      241 cgctattttt attccactac gttttatgtc ggagacaaag gggttttgct gtttgatgct
      301 ccggagggtc gcggtaaata tttattacag gcaattcgtg atgtgactcc gttacccgta
      361 acagcgctgg tttattctca ttaccatgtg gatcacattg gcgatagttc attttggaac
      421 gatgaggcga agaaagaagg cgtgaatctg cgtattattg ccagtaaagc cactgctgaa
      481 aaaatgcaat ttatgaacag tcgcttaccc gtcgccactc aggttttaag taaaaaagat
      541 gaccagttta aatttgaaaa gcagaccatt gaattacacc gatttgttaa agcaggacat
      601 accgacgacc attctgtatg gttgctaaaa caggaaaaag tagctcacag cccggatctc
      661 ctgaatcctg accaactgcc gatgatgggc ttcgccgtat cggatacgtt ggtttaccat
      721 gacagtaatt tgcgtcaggt tgaaatgctt gactggaaat attttattgg cgggcatggt
      781 aatattggtt cacacgatga tttcaaattt caacgccagt ttcttaatga tcttagggat
      841 acgacaatta aagtcagaaa agaagaaagt tttggtaaat ttatgaataa aacggcaaat
      901 aatcatgctg attttgcgcg agcccagcgc gaagcaatta ttaagaaagt aactgaagta
      961 ttacgtccga aatatggcca tatgtatggc tacgacgcat ctatgcctgc taatatcgaa
     1021 atggcaataa gattggtcgg ttcttattac taagataaaa tcaacttgca agagattgta
     1081 attatattgc aaagtgaaaa ttaacctcgg tagcgagata ttaccgaggt taatattggc
     1141 atacattaca cctttatcag gcacttaaac gatacatcat atcttatctg gattattaga
     1201 tgacgagcca tacgcaccag gtaattaaga atccggtaaa accgagaatg gtcgttaaca
     1261 ctgtccaggt tttcagaccg tctgctaccg acaaccccag atatttggtc acaatccaga
     1321 accctgagtc attaatatgt gacgcgccaa gcccaccaaa gcaggctgcc agcgtcacca
     1381 atacgcactg aatcggattt aatcccatca ccgcttctga gagtaacccg ccggttgtca
     1441 ggattgctac ggttgctgac ccctgcgatg cacgcagcgc cagtgaaata ataaatgcgg
     1501 ctggtaacag aggcaggtca atcatttgta acatgttggc aagggcttta ccgacgcccg
     1561 attccaccag cactttgcca aatacccctc cagcaccagt aaccaaaatc actaccgccg
     1621 cagtgggaag ggcagagccc ataatgtcgc tggtgtgttg taagctccag ccgcgacgta
     1681 aagccaataa ccagaatgcc agcaccagcg caatcattag agctaccatt ggtgagccga
     1741 tcaactgtag cgtaccaagc aggggatgcg aaggcggcat cagtgttgca gaaaccgtac
     1801 ccgccatgat aatcgcgata ggaataacaa ttagcgaggt gaccagcgcg acgcccggtg
     1861 gatttatttt atcgcttaat tttgtcgcgc cttcctcact ggccggagcc agttgcatct
     1921 gttccagtac ctcgaccgac atcgcatatt ggcgcttatt gattattttc gctgcaaagt
     1981 agccaacaat ccctacggga atagaaatcg caataccgat gatggttagc cagccgatgt
     2041 ctgcgtggag taaccccgct gcggcgacag ggcctggatg tggcggtacc gccacgtgaa
     2101 cagtgagcat gatcccagcg acaggcaggc caaatttgag tggtgatatt ttggcaacct
     2161 tggcaaaacc gtaaatgatt ggcgcaagaa taataaagcc gacatcaaag aagacgggaa
     2221 taccgaggaa gaacgctgcc agagtcagcg cagcgatagt tcgtttgtca cctaacttgc
     2281 gactgaaata attagccagt gactctgcac caccagagtg ttcgatcata cgccccagca
     2341 tagcgcccag accaataata atagtgacgg aaccaagtac accgcccatc ccggcgatca
     2401 tcactttacc cacttcgccc gccggtatac ccgccgcaag tgcgactaac aggctgacga
     2461 ggagcagagc aacgaatggt tgtacctttg ccttgatgac cagtagcaac agcatgatta
     2521 cgccagctaa cgcaatgcat aacaatgtaa ttgtggacat gggaaaccct gtctgaaagt
     2581 tatagttaac ctaccccatc cgtagatggg gggatgtatg ggtacgttgt aattagggat
     2641 tgagcgaatt agcgccaggc gtcaaaccag ccaagccctt cttcggtgag gccacgaggt
     2701 ttgtattcac aaccgatcca gccctgatat cccacttcat cgaacaggcg gaacagccac
     2761 ggatagttga tttctccatc gtccggttca tgtcgatcag gtagtccggc aatttgtacg
     2821 tgcgcatatt tcccggcgta gtcgcggatt aaatgtgtca ggttgccatc tactttttgc
     2881 gcatggaaag tatccagttg aataaatacg ttatctcgcg caacctcttc aacaatagcc
     2941 agtgcctgat actggctgga gaagagataa tgaggcttaa cgtcggggct gagtgcttca
     3001 accaatattc gcttgccgtg tggcgcaaag cggtcggcag cgtagcggag attatcaata
     3061 aatactgccc ggtaccgttc agcatcttcg ccagcgggca cgacgcctgc catcacatgg
     3121 acttgttcac aattgagcgc caatgcatat tccagtgcca ggtcgatgtc tgcgcgtgct
     3181 tcgtgctcac gtccgggaag ggcggataat ccccattctc ccgcattaat atctccggga
     3241 gcggtattga acagcgccag tgtcagatgg ttttgcacca gttgctgctg gatttgctgg
     3301 gtggagtagt catagggaaa cagaaactcc acagcatcga acccggcttt tcgcgctgcg
     3361 gcgaagcgtt caataaaagg cacttcggtg aacatcatgg ataaattagc tgcaaaacga
     3421 ggcattgcat taactcctta attccgcaat ttcacctgcg gtcagataac ggatcgggcg
     3481 gtcaccgaga ataaaaatca gctttgccgt ttcctccagc tcttccatat tgttggcggc
     3541 ttcttgcagg ctttcaccgc aaaccactgg gccatgattt gccagtaaaa aagcctgatt
     3601 gtctgctgcc agttccgcca gatcctgtgc gatgcgttta tcgcccggtc ggtaataggg
     3661 caccagcggg acgtttccca tccgcatcac cacgtatggt gtgaacggac gaataacgtt
     3721 attgctgtcc agcccttgca ggcaggaaag cgccgtcgac cacgtactgt gcaaatgcac
     3781 taccgcttta cagcgcggat tgttgcgata cagcgccaga tgaaagagca cctctttcga
     3841 gggtttgtca ccacttaacc attcgccatc cgcggtgact ttggaaagcc gctgcggatc
     3901 gagattgccc aggcatgaac ctgtcggtgt cgccagtaaa ttcccgtcag gtaaaagcag
     3961 cgacagattg ccagccgaac cggttgcata tccgcgctga aagaatgaac tggcaatccg
     4021 cgtcatctcc tctcgcaaag actgctctac ttttgcgaaa tcgctcatga taaaaactct
     4081 ctttgggctc gtgaaaaaaa ggcttcatca ccgaagttgc cagatttaag ggcgagtgag
     4141 acaggcttat ccagtgcgtt tacccacggc acgccagggg aaatggttgg gccaatatga
     4201 aaccctttta ttcccaggct ctgtgtgact acgccggagg tctcaccgcc tgcgacaata
     4261 aagcgtgtca cgccttccgc tgctaaccgc gccgctagtt gagaaaacag tgtttctact
     4321 gcctgactgg ctttttgtgc accgtattgc tgttgaattg ctgccaatgc gtcagtgctt
     4381 gcggtggcaa aaaccagtgg agcaagtaca ctttcctggc ccagaaccca ctctgccagt
     4441 tcgtgtgcat aagcggccag agtttcggtt gagaggcagc gtgccacatc aacctcacgg
     4501 gctggtgcaa tttgacggta atgtgccacc tggcggttgg tcatttgaga gcatgaaccg
     4561 gagagcacta cgccgcgccc agcgagcgga cgccctgctt cgcgagcctg gttaccgttt
     4621 tcttgcgccc actgccgggc caggccaatg gccagaccag aaccgcccgt taccagtggg
     4681 gcatcgcgca aggcttcccc ctgaatttcc agatggtgtt cggtcagcgc atcaagcacc
     4741 gcgtagcgat agccctcttg ctgtaagcga gccagctcct gacgaacggc tttcacacct
     4801 tgttcgaaaa catgtgctga aacgacgccg cagcgccctg tggattgcga ttcaaccaga
     4861 cggggaagat agctgtcggt catgggattt accgggtgat ggcgcatccc ggattcggcc
     4921 agcagttgat tcattacgaa caaatacccc tgataaaccg tacgtccgtt gaccggcagg
     4981 gccggagaga agaccgtaaa cggcgtgtcg agagcatcca ttaatgcatc ggtaaccggg
     5041 ccgatattcc ctttcgccgt actgtcgaaa gtagagcagt atttgaaata aatctgtttg
     5101 caaccttgct gttgcaacca gctcagagcc gccagcgatt gctgtgtggc ttcaaccacc
     5161 ggacaggagc gcgttttcag gctgatcacc agtgcgtcga ttgcttccgg cattttacct
     5221 gttggaacac cgttaatttg taccgttggt agaccgtttt ccaccagaaa actggcgata
     5281 tccgtcgcgc cggtaaaatc atcggcgata acgccaatct tgatcatgat ttcgctcccg
     5341 gtagagtgat gccagagaaa atcttgataa ctgcgctatc gtcttctttc ccgtaacccg
     5401 cgttactggc gctggtgaac atattcaatg ctgttgaggc caatggcagc gggaagtgca
     5461 gggctttggc ggtatcggca accagaccaa gatccttaac aaaaatatcg acggctgaat
     5521 gcggggtgta atcgccatcc accacatgac gcatccggtt ttcgaacatc catgaatttc
     5581 cggcggcatt ggtcacgacg tcatacatca catccagcgg gatccccgca cgggctgcaa
     5641 gcgccatcgc ttcggcgccg gcagcaatat gtacgcccgc taacaactgg tggataattt
     5701 ttacggtcga acccagtccc ggttctgctc cgatgcgata aacttttccg gcaacggctt
     5761 ccagcacggg tgccagtcgt tcaaaggcaa tatcgctacc ggaggccatg acagtcattt
     5821 caccgttagc ggcttttacc gcaccaccag aaaccggcgc atccagcatt tccagaccga
     5881 atccagccag agcggtagca atttcttgcg catcagcact ggcgatagtg gaagaaacca
     5941 ttactgccgt accgggtttc agatgttgtg caacgccttt ttcaccaaac agcacctgtt
     6001 taacctgggt cgcattgacc accagcacca gcagagcgtc cagtttttcg gcaaacgtcg
     6061 cggcgttatc agaaaccccg caagcaccag cctctttcaa cgtagcgcag gtattgctgt
     6121 tcaggtctgc gccccaggta gaaagacctg cgcggacaca tgacagtgct gctcccattc
     6181 ccattgaccc taagccaacg ataccgacat gaaactccga tcccgttttc atctgctctc
     6241 cttgttaatt taagtgatat tttgttttat gttgtgaata taagcgcgga aagataaaga
     6301 tatggtgagc tgattcacaa aagttaacat tgcgtgttat tttatgtgaa ctaagcgtta
     6361 gttgcgccgc gcacgtttcg caggcaaata gcgtagaatg tcagcaggac aaaggaagga
     6421 gcaaaagttg atacccgtag agcgtcgcca aatcatcctt gagatggtag ctgaaaaagg
     6481 cattgttagt attgctgaac taacggacag aatgaatgtg tcacatatga ccattcgtcg
     6541 ggatttacaa aaactggagc agcagggagc cgttgtgctg gtgtccgggg gcgtccagtc
     6601 tccgggacgc gtggcgcatg aaccttctca tcaggtaaaa actgcgctgg caatgacgca
     6661 aaaagcggct attggcaagc tggcggcaag tcttgttcag ccgggacgtt gtatctatct
     6721 ggatgcggga acgaccacgt tagcgattgc acagcatctg actcacatgg agccactcac
     6781 agtggttact aacgacttcg ttattgcgga ctacttgctc gacaacagta attgcacaat
     6841 tattcacact ggcggcgcag tgtgtcggga aaaccgttcc tgtgttgggg aagccgctgc
     6901 gaccatgctg cgtagcctga tgattgatca ggcttttatt tctgcatcgt catggagtgt
     6961 gcgggggatc tctacgccag cagaagataa agtcacggtg aaacgggcga ttgccagtgc
     7021 cagccgccag cgagttttgg tctgtgatgc gacgaaatat ggacaggtgg cgacatggct
     7081 ggcgttacca ttaagcgagt ttgatcagat cattactgac gatggtctgc cggagagtgc
     7141 cagccgcgcg ctggcgaagc tggatctctc tttgctggta gcgaaaaatg aataatgacc
     7201 tgcaataacg ccgggttact catgcttcac agaagaagca tgagactact ttattttata
     7261 aaatgacagc cgcccgcttt tcggcgatcc ggtatctata taaatctggt tagcgaacgt
     7321 ctgaatgtta tcaaacatca tatgaccaaa tataaagaag tcggcgccgt ttattttttg
     7381 taattcgcta ttaagtgatt tctgcacatg atcaacaggc cagagtaatt cgctctccgc
     7441 tatttctttg ccaaactgat actccttccc cggataatct gcatgtgcga tgacatattt
     7501 tatggtgtcg ttagtgattt caataatatg tggaaggtga tggaatttca gcaacagatc
     7561 tattgcctct tgttgctctg aatcatttaa atcgaaaaac cagtcaccac cgctggcaag
     7621 ccacatattt ccatcgccag tttcgaatgc atcaagcgcc atcgcttcgt ggttgccttt
     7681 gacggagata aaccagggct ggtttaacag gcgcagcacg ttaagactct cgggcccacg
     7741 atcgatgtta tcgcctaccg aaataagtaa gtcggtttca gggcaaaaag agagttgatg
     7801 taagcgggat tgtaataatt gatagtcacc atgaatatca ccaacggtcc atatatggcg
     7861 atagtgatgg gcgttgattt tttggtagcg tgtagatggc atggttttac cctgtaaaat
     7921 aagctttcct attatacagg gtatttttat ttgattcgtc agttgttgtt aatattcccg
     7981 atagcaaaag actatcggga attattatta caccagactc ttcaagcgat aaatccattc
     8041 cagcgcctga cgcggagtca gtgaatccgg gtcgaggttt tccagtgcct cgaccgcagg
     8101 cgaagtttct tccggtacgg acagcaaaga catttgtgta ccatccactt gcgtagcagc
     8161 ggcgttcggc gaaatgcttt ccagctcacg cagtttttgc cgtgcgcgct taataacctc
     8221 ttttggcacg cctgccagcg ctgcaaccgc caggccgtag cttttgctcg ccgcgccatc
     8281 ctgcacgcta tgcataaagg caatggtgtc gccgtgctcc agtgcatcga gatgcacgtt
     8341 ggcgacgcct tccattttct ccggtagctg ggtcagctcg aaatagtggg tagcaaacag
     8401 cgtcaacgct ttgatcttat ttgccagatt ttccgcgcat gcccacgcca gcgacagacc
     8461 atcgtaagtg gacgttccac gcccgatctc atccatcaac accagactgt attcggtggc
     8521 gttatgtaaa atattggcgg tttcggtcat ctccaccata aaggttgaac gcccggaagc
     8581 cagatcatcc gcagcaccta cgcgggtaaa gatacggtca atcgggccaa tctcgacttt
     8641 ttgcgccggt acgtagctgc cgatatacgc catcagcgca atcaacgcgg tctggcgcat
     8701 ataggtactt ttaccgccca tgttcggacc ggtaatgatc aacatgcgac gctgcggcga
     8761 caggttcagc gggttagcga taaacggctc attcagtact tgttcaacta ccggatggcg
     8821 accttcggta atacgaatgc caggtttatc aatgaaggtc gggcaggtat agttcagggt
     8881 ataggcccgt tccgccaggt tcgccagcac gtcgagttcc gccagcgcgc tcgcgctctg
     8941 ttgcaacgct tccagatgcg gcaacagcag gtcgaacagc tcttcataaa gttgtttttc
     9001 cagagccagt gctttgcctt ttgaggtgag aactttatct tcgtactctt ttagctctgg
     9061 aatgatgtag cgctcggcgt ttttcagcgt ctggcgacgc atgtagttga ttggtgccag
     9121 atggctttgc ccacggctga tttgaatgta gtagccgtgc accgcattaa agccaacttt
     9181 cagcgtgtcc aggccggtac gttcacgctc gcggacttcc agacgctcca gataatcggt
     9241 cgcgccgtca gccagcgcgc gccactcatc cagctcttcg ttatagcccg atgcgataac
     9301 accaccgtcg cgtaccagca ccggtggtgt gtcgatgatt gctcgctcca gcagatcgcg
     9361 cagctcggca aactcgccca tcttctcacg cagcgcttgt accggtgcac tatcgacatt
     9421 ttctaactgc gcacgcaact ctggcagttg ctggaaagcg tgacgcatac gggccagatc
     9481 gcgtgggcga gcggtacgca acgccagacg cgccagaata cgttccaggt cgcccacctg
     9541 acgcagtacc ggctgtaact cggcggtgaa atcctgcaat gcgccaatag tttgctggcg
     9601 ctcaagcaac acgcgggtat cgcgcactgg catatgcagc cagcgtttca gcatacgact
     9661 acccatcggc gttacagtgc agtcgagcac tgaagccagc gtattttccg caccgccggc
     9721 caggttctga gtaatttcca ggttacgacg cgtcgcggca tccataatga tgctgtcctg
     9781 ctgacgttcc atagtgatag aacgaatatg cggcagggtc gtgcgttggg tatctttcgc
     9841 atactgcaac agacaaccgg cagcacaaag tccgcgtggt gcgttctcca caccaaaacc
     9901 gaccagatct cgggtgccaa attgcaggtt caattgctgg cgagcggtgt cgatttcaaa
     9961 ctcccacagc gggcgacgac gcaggccgcg gcgaccttca atcagcgaca tctcggcgaa
    10021 atcttccgca tacagtaatt ccgctggatt cgtacgttgc agttctgccg ccatcgtttc
    10081 gcggtccgcc ggttcgctca ggcgaaaacg accggaactg atatccagcg ttgcgtagcc
    10141 gaacccttta ctgtcctgcc agatagccgc cagcagattg tcctggcgct cctgtaacag
    10201 tgcttcatcg ctgatggtgc ccggcgtaac gatacggaca actttgcgct cgaccggccc
    10261 tttgctggtc gccggatcgc caatctgttc acaaatagca accgattcgc cctggttcac
    10321 cagtttggcg aggtagtttt ccaccgcatg gtagggaatc cccgccatcg ggattggctc
    10381 tcccgctgaa gcaccgcgtt tggtcagtga aatatccagc agttgcgacg cccgttttgc
    10441 gtcgtcataa aacagttcat aaaagtcgcc catccggtaa aacagcagga tctcgggatg
    10501 ctgggctttc agcttgagat attgctgcat catgggcgta tgggcgtcga aattttctat
    10561 tgtactcatg gggttaagtc cggctccctg atgttaaatg tagtgtgatg gtttttattt
    10621 tcgttgtaat cacataaggc gcacatgata ccggagttaa tcatttcagg ggaagacgcg
    10681 aaaggtgagt tcttgatgtc ggttccgcga aaaatgctgc tttacagctt ttgcgtgatg
    10741 acgatgaaac aagatcgcca aattcgtcgg ttgaagaaac ctcctccttc agagccatag
    10801 tcccctttaa aattaagttg taatataaat gcaccttaga gtatggaggc agcatgtccg
    10861 gcaagcgtat ctctcgtgaa aaactgacga ttaaaaaaat gatcgatctt tatcaagcga
    10921 aatgcccgca ggcgtcagca gagccggagc attacgaggc gttgtttgct tacgcgcaaa
    10981 agcggctgga taaatgtgtg ttcggtgaag agaaaccagc ctgtaagcag tgtccggtgc
    11041 actgttacca gcctgcaaag cgtgaagaga tgaaacagat tatgcgttgg gccgggccga
    11101 gaatgctgtg gcgtcatccg atcttaaccg tgcgtcatct catcgatgac aagcgtccgg
    11161 tgccggagtt gccagaaaaa tatcgtccaa agaaataaat ctgccggaga gccatactct
    11221 ccggcaaata aggcgtttac gctttggtgg caatcaggat ggctgatgct ttgatcaatg
    11281 caataacgcg cgagccagtg gtgagtttca tttcatcctg actttcattt gttaccactg
    11341 ccacaatttc aaaacccgca tccgttttga tatggacagt ggcgttcact gcgccttcgg
    11401 tgattgtcga cacgctgcca gcaaactggt tgcgagcaga gaatttcagt ccacagtctt
    11461 ccgttgccag cgttacccac ggggctttaa tcagggcaat cgcttctttc cctttcgcca
    11521 gacccagcgc ttgctggctg ctgtgtgtaa caattgccac cagctttgca ccacctgcca
    11581 gcgtcagttc tacttcatca ttcacagcgc ccattgctac tgcgctaact gttccggtta
    11641 attgatttct tgctgaaact gccatactgc ctcctttcat tgaatgttga tcgagtataa
    11701 ccacatctgt taaatcaatg ccgatttatc aattcccagt cgcttcattc gtgacagcag
    11761 agtcgtgcgt ttcagcccca gacgttgcgc agcgccttta ggcccggcaa ccacgccgtt
    11821 tgtttctttc agcacgcgaa caatcaactg atattcatct tcgccctcct gggcgacaac
    11881 cgttgcggca ggcggcgttt caggttctgg taaagcaata tctggcaatg acagctgcag
    11941 cacgttgccg cgtgttagca ataccgcgcg ctcaatgacg ttttccagct cacgcacgtt
    12001 gcccggccat tccatgttgc tcaggatgcg cagcgtctcg gcaggaatgc tgtcgatatt
    12061 gcgccccaga cgacgggcaa ttttgaaggt aaaggctttc gccagcagcg gaatatcttc
    12121 cggacgctcg cgtagtggcg gcaggtggat cgggaatacg ttcagacggt aatagagatc
    12181 gctacggaac tctcggtcgg cgaccatttt tttcagatcg cggttagtcg cggcaattaa
    12241 acgtacgtcc gtctgaatga ttttgttgct gccgagacgt tcaaactcct gttcctgtaa
    12301 tacacgcagc aacttcggct gtaactccag cggcatatcg cccacttcat cgaggaatag
    12361 tgagctttta tccgccagtt caaaacgacc aatgcgctga gcgctggcac cggtaaaagc
    12421 cccacgctca tgaccaaata gatcgctttc cagcaatccg gcaggcatcg ccgcgcagtt
    12481 cattttgacc atgcggcgat tattacgccc actgagatta tggatcgcgc gggcaatcag
    12541 ctctttacct gtaccagttt caccgaggat cagaacggta ctgtcacttt gcgccaccat
    12601 ttccacttgt ttaagtacgc tgtacatggc ttcgctgcgg ccaataatct cgccaaattc
    12661 actatcaaca ttgttgagct gctcggtcag ggcgaggttt tcatcaacca gccgttcttt
    12721 cagacgatga atttcctgat aggcgagggc gttatcgaca gcgattgcca cacgttcggc
    12781 aatctggcgc agtaagctca gattggtagt ggtaaatact ttctcttcgc attgcgccag
    12841 ttttagcacg cccagcatgg tgtcgccaga catcagcggt aacaggcaca aggtttgaat
    12901 ctggttgccc caggtgttga acaacatgcg ttcatagggg gctaaatcgt cccgctcgtg
    12961 gagattaatc aacagcatct ctttactttt gaagacgcgt tcggtgaggg ttccggcttc
    13021 atcgacttcg ctctgttcgt gagcgggatg ctgtttatca agatagtgag tggagtagat
    13081 gttgagtttg tttttacggt ggctgcgtaa gacgatgctg atatcgtcaa tgtcgaagta
    13141 gtagtggatt tctttggcga cttcgctgac cagttcgtcc atatccaggc gggaaagcac
    13201 cgcgttagtg atggcgacca ggatgcggaa gttatcgcgt tcccggcata acaactcata
    13261 gtcgacattg ttaacgacgc ggctctggat ttgttcggtg acgacagaaa cgatctgcgt
    13321 aaatgtttgc agacgattga actctttttc gctccagggg cgatcgtcat aacgaataaa
    13381 ttcacagcca ccaaaaatat gcccttccgc cgccagtggc atcaggcaat agtgaccaaa
    13441 tttggggtat agcccacctg tggccagctg cggccaggtt tcacaaaatt cttcgtaact
    13501 gcaatgcagc gtatcagggc gcgacaaaat gctgcgtacc ggaccgtgtg ccagaacagt
    13561 ttcgtcttca tatttaatgg gggtgtcttt ttcacgcgat gcgtaataag acgcacgttg
    13621 agtctgcgct tgccacaaca caatcgccgc gttgtcggcg agcgcagaac gctttaccag
    13681 ttgcgaaaga gcctcacaca gcgaggccag atcgggctgc tgcaatagtg tccgagtgat
    13741 gtcgaacaac ccttgctgtc cgagatcact catcggtgta tatgacattt tgttcttcca
    13801 ggaaagcacc gcaaagcggt gcaaaaggtc attgactaac tataggagat ttagaatttt
    13861 attagcatat acgcggaagc ggttcggcgt gcggtagatc gagggttcgt ttcacgccat
    13921 acagaccggc aagacgaaca cctttacgtt ctactacttc gccaatcagc gccgcatctt
    13981 ttcccaatgg atgggaatgt aacgccgcca gcacttgctc tgctgcgttg cgttcaacgg
    14041 cgatcaccag tttgccttcg ttggcaaagt taagggcgtc cagtcccagc agttcgcaaa
    14101 cgccgcgcac agcaggttta accggcagtg ccgcttctga aagttcaata ccacaaccgc
    14161 aggctgccgc gaactcatga accaccgcgt ttacaccacc acgggtggca tcacgcagcg
    14221 ctttcacgcc gggaatgtca cgcagcgtct gaataagcgg cgtcagcacc gcgcagtcgc
    14281 tgaccagttc gccatccagc cctagttgct cacgcaggtt aaggatagtc gccccgtggt
    14341 cgccgagtgt accgctaacc agcaatacat cgcctgcggt tagcgtctgt gcgccccagt
    14401 gaatattcgc cggaattgcg cccatgccag cggtgttgat aaacagttta tctgccgcgc
    14461 cgcgctgtac cactttggta tcgccagtaa cgatggcaat gcctgccgcg cgggcggttt
    14521 ctgccatgct ggtcactacg gctttcagtg tctccatcgg caatccttct tcgaggataa
    14581 agccacagga gagatagcgc ggaattgcgc cactgaccgc aacgtcattg gcggtgccgc
    14641 aaatcgccag cttgccgata ttaccgccag gaaagaacag cgggtcaatg acgtaactgt
    14701 cggttgagaa cgccagacgg tcgccttcag ctaccagctg tgccaaatca agacgggcct
    14761 gatcttcctg ctctgccagc cacgggttgg aaaaggcttc cataaacagg ctgttgatta
    14821 attgctgcat cgcctggccg ccgctaccgt gggcgagttg gatattattc acgcttcact
    14881 ctcctgctga cgatactgat accacgcggc gcacgctcct tcggaggaaa ccatcagcgc
    14941 accaaacgcg gtttgaggat tacaggtgtt accaaacagc gggcattgat gcggcttaca
    15001 tttgcccgtc aagacttcac cacaacgcgc acgcgggtca tcgcagacct gctgcggtgc
    15061 cgggcggaaa tgtgtttcgg catcgaatcg ttgataatcc ggcgtcaggt gcacgccaga
    15121 agattcaatc acgcctaagc cgcgccattc actgtcgccg ttgacacaga acacatcggc
    15181 aatcgcctgt tgcgccagca ggttaccggc atccggtacc acccgacgat actgattctc
    15241 taccttgctg tgggccgcta ttttctgctc caccagcatc accacgcctt gaagtagatc
    15301 aaggggttcg aaaccagcga ccaccagcgg gcgatgaaaa tcgctggcga taaaattata
    15361 ggcatcagtg ccgataacca tactgacgtg gcccggcgcg aggaacgcgt cgataccgtt
    15421 atctggctgt tccagcaaac tgcgcagcgt cgggataagc gtaatgtgct ggcagaagaa
    15481 gtaaaaattc tgcacatcgc gggctttcgc ctgttgcaga gtgatggcgg tggtcggcat
    15541 ggtggtttca aaacctaagc cgaagaatac cactttacgg gttggattct cctgcgccag
    15601 tttcaacgca tccattggcg agtaaacgat gcgcacatcg gcaccgcgtg cttttgcctg
    15661 caacagcgac ccctgtttcc ctggtacgcg catggcatcg ccaaaggtac agaagatgac
    15721 ttccggatgg ctggcaatct ccacgcaggt gtcgattcta cccattggca gtacgcacac
    15781 agggcaaccc ggaccgtgga taaactcaac gttttccggt agcaactggt cgaggccgaa
    15841 tttaaaaatg gcgtgggtat gaccgccaca cacttccata atccgcagag ggcgttcggc
    15901 ggtgtaagag agatgtgaag cacgttcgcg cagatgctca attaactgca tcacctgttc
    15961 cggcgcgcga tattcatcaa caaaacgcat tatttttcct cgccatacaa cagcgcgccg
    16021 acatctggct caacgtcaaa catgttttgt aaggcgtcga gagtgtcgcg tgcttcggct
    16081 tcattaatta cgctcatggc aaagccaacg tgaaccagta cccactggcc cacgcgtggc
    16141 tgaccgtttt catcgcagct gccgactaac gttaaatcga catcgcgctg aatgccgcag
    16201 acgtcgactt tcgcctggtt gccgtcaatg gtgcgtatct ggccgggaac gcctatgcac
    16261 atcgctgtgt ctccagccag ttcagccact ggtccatccc ttcgccgctg gtggcggaaa
    16321 taaggatgat ttcaatttct ggattgactt cacgggcgca ggcgatgcac ttctcaacgt
    16381 caaagttgag atacggcagc aggtcaactt tgttgagcag catcagcgag gcggcggcaa
    16441 acatatgcgg atatttcagc ggtttgtctt caccttcggt aacggaaagc accgccactt
    16501 tgtgtttttc accgagatcg aagctggccg ggcacacgag gttgccgacg ttttcgataa
    16561 acagaatacc gttatcgtcc agtggcaggc gcggcgcggc gtcggcaatc atctgtgcgt
    16621 caagatggca gcctttaccg gtgttcacct gaatcgctgg cgtgccggta gcacgaatgc
    16681 gcgcggcatc gttcacggtt tgctggtcac cttcaataac cgcgcacgga acgctgtctt
    16741 tcaggcgcat taaagtttcc gtcagcaggg tggttttacc ggaaccaggg ctggaaacca
    16801 ggttgagcac cagttgcttg cgggcagcaa agcgcgcgcg gttgcgttca gccagacggt
    16861 tatttttgtc cagcacgtca atttcgactt ccagcatccg acgctggctc atgcccggcg
    16921 cgtgagtgcc cgcttcgcca tgaccgtaat gcaggtcgcc ttcttcagtc tggctggggg
    16981 taaattcagg cgctttaatg ccggtgattt tcatcttcgg gcgtgccgcc ggggcaaatg
    17041 gcgcgctacg aaacgcggaa tgagggttat gttcatcacc ctcaatatag aggttgcctt
    17101 caccgcaacc gcatgttgta cacatcgctc actcctggtc tatttctatc cgccgaatct
    17161 gtaaaccgtc gtctgccaca atctgtagca tgtcaccatg acacagtgga cagcggcgga
    17221 cgcgctgggt cagtagcgtc acatactgtt ggcatgtttc acaccagcat tcggcctctt
    17281 gttcttcgag gtgcagttta caaccttccg ccacgctgcc gcggcaaacc agatcaaaac
    17341 aaaaggcaag agagctggtt tcgacacaag aaaatgcgcc aattttgagc cagaccccag
    17401 ttacgcgttt tgcgccgtgt tttgcggcct gctgttcgat caattccagt gcccgttggc
    17461 agagggttat ttcgtgcata tcgcctccca ttaactattg ccagctacaa gcaataattg
    17521 tgccagtgtt gattatccct gcggtgaata atgtcgatga tgtcgaaatg acacgtcgac
    17581 acggcgacga aattcatctt tagcttagca aatcgcttta ataacaatga attaaaagtt
    17641 ggcacagaaa atgcttaaag ctggcatctc tgttaaacgg gtaacctgac aatgactatt
    17701 tgggaaataa gcgagaaagc cgattacatc gcacagcggc atcgtcgcct acaggaccag
    17761 tggcacatct actgcaattc gctggttcag gggatcacgt tatcgaaagc gcgcctgcat
    17821 cacgccatga gctgcgcgcc ggacaaagaa ctctgtttcg tcctttttga acattttcgc
    17881 atttacgtca ccctggcgga tggctttaac agccacacca tcgagtatta cgtcgaaaca
    17941 aaagatggcg aagacaaaca gcggattgcg caggcgcaac tgagcattga cggcatgatt
    18001 gatggcaagg tcaacatccg cgatcgcgaa caggttctgg aacactatct cgaaaaaatc
    18061 gctggcgttt acgacagctt atataccgcc attgaaaaca atgtgccggt gaatttaagc
    18121 caactggtaa agggacaaag cccggcagca tgagctgagg ctttgcccgt tttgcaggcg
    18181 ttacgcctgt ttggggatgg gcgtgtcgat gagtgtcgaa aatgacattt catcggcatg
    18241 ttttcgtcaa aaatgacaat cacctgagga atgcctggtg aatcgttttg taattgctga
    18301 ctccacgctc tgtatcggct gccacacttg tgaggctgcc tgttcagaga cgcatcgcca
    18361 gcacggcctg caatcaatgc cgcgcctgag agtgatgctc aatgaaaaag aatctgcgcc
    18421 gcagctctgt caccactgtg aagatgcacc ctgcgcaacg gtctgcccgg ttaacgccat
    18481 cactcgtgtt gatggtgccg tgcagctgaa tgaaagcttg tgcgtaagct gcaaactgtg
    18541 tggcatcgcc tgcccgtttg gcgcaattga attttccggc agccgtccgc tggatattcc
    18601 tgcaaatgcc aataccccga aagcgccacc ggcaccgcct gctccggcgc gtgtcagcac
    18661 attgcttgac tgggtgccag gtattcgcgc gatcgccgtc aaatgtgacc tttgtagctt
    18721 tgatgaacaa ggtccggcct gcgtgcggat gtgcccgact aaagccctgc atctggtgga
    18781 taacaccgat atcgcccgcg tcagcaaacg taagcgtgag ctgactttta acacggactt
    18841 tggcgatctc accttgtttc agcaggctca aagtggagag gctaaatgag cgcaatttcc
    18901 ctgatcaata gcggcgtggc gtggtttgtc gccgccgctg ttctggcatt tctcttttct
    18961 tttcaaaaag cgctaagtgg ctggatagcc ggaattggcg gcgcggtggg tagcctgtat
    19021 acggcagccg cgggcttcac tgtgctgact ggcgcggttg gcgtgagcgg tgcgctgtcg
    19081 ctggtaagct acgatgtgca aatctctccg cttaatgcga tttggctgat tacactcggc
    19141 ctatgcggtc tgtttgtcag cctctacaac attgactggc atcgccacgc gcaggtgaag
    19201 tgcaacggct tgcagatcaa tatgttgatg gctgccgccg tctgtgccgt catcgccagc
    19261 aacctcggca tgttcgtggt aatggctgaa attatggcct tgtgcgcggt gttccttacc
    19321 agcaacagca aagagggcaa actgtggttt gcgctggggc gtcttggcac tctgctgctg
    19381 gcgattgcct gctggctgct gtggcagcgt tacggcacgc tggatctgcg cctgctggat
    19441 atgcgtatgc aacagctgcc gctcggttcc gatatctggc tgctcggtgt gattggcttt
    19501 ggcctgctgg ccgggattat tccgctgcac ggctgggtgc cacaggcaca tgctaacgcc
    19561 tctgcgccag ctgccgcgct gttttccacg gtagtcatga aaattggcct gctgggcatt
    19621 ttaactctgt cactgctggg cggtaatgca ccgctgtggt gggggatcgc actgctggta
    19681 ctcggcatga tcaccgcatt cgtcggcggt ctgtatgcgc tgatggaaca taatatccaa
    19741 cgtctgctgg cttaccacac cctggagaat atcggcatta tcctgctggg gctgggcgct
    19801 ggcgtaacgg gtatcgcgct cgaacaaccg gcgctgattg cccttggtct ggttggtggt
    19861 ctgtaccatc tgattaacca tagcctgttc aagagcgtac tgttccttgg tgcggggagc
    19921 gtctggttcc gtaccggtca tcgcgatatc gaaaaactcg gtggtattgg caagaaaatg
    19981 ccggttatct ccatcgccat gttagtcggg ctgatggcaa tggctgcgct gccgccgctg
    20041 aacggttttg ccggggaatg ggttatctat caatccttct tcaaactgag caatagtggc
    20101 gcgtttgttg cccgtcttct cgggccgctg ctcgccgtgg ggctggcaat taccggtgcg
    20161 ctggcggtga tgtgtatggc gaaagtttat ggcgtcactt tccttggcgc gccgcgtacc
    20221 aaagaggccg aaaacgccac ctgtgcgccg ctcctgatga gcgtaagcgt agtggcactg
    20281 gcgatttgct gcgtaattgg cggtgttgct gcgccgtggc tactgccgat gctctctgct
    20341 gctgtacctc tgccgctgga gcctgctaac accaccgttt ctcaaccaat gatcacgttg
    20401 ctgctgattg cctgcccgct gctgccattc atcattatgg cgatttgcaa aggcgatcgt
    20461 ttgccgtcgc gttcccgcgg cgcggcctgg gtgtgcggct acgaccatga aaaatcaatg
    20521 gtgattaccg ctcacggttt tgccatgccg gtgaaacagg cgtttgcgcc ggtgctgaaa
    20581 ctacgcaaat ggctgaatcc ggtgtctctg gtgccgggct ggcagtgcga ggggagtgcg
    20641 ttgctgttcc gccggatggc gctggttgaa ctggcggtgc tggtggtgat tattgtttca
    20701 cgaggagcct gagaatgagt gttttatatc cgttaattca ggcgctggtg ctatttgccg
    20761 ttgcgccgct gctctccggt attacccgtg tggcgcgtgc ccgtctgcat aaccgtcgcg
    20821 ggccgggcgt gttgcaggag tatcgcgaca ttatcaaact gctgggccgc cagagcgttg
    20881 gcccggatgc ctccggctgg gtgttccgcc tgacgccgta tgtgatggtg ggcgtcatgc
    20941 tgactatcgc cactgcgctg ccggtggtga ccgtcggctc tccgctgccg caactgggcg
    21001 atttgatcac cttactgtat ctcttcgcca tcgcgcgttt cttctttgcc atttctggtc
    21061 tggatactgg tagcccgttt accgctatcg gcgcgagccg tgaagcgatg cttggcgtgt
    21121 tggttgaacc gatgctgctg cttggtctgt gggtcgccgc acaggttgcc ggttccacca
    21181 acatcagcaa catcaccgac accgtttatc actggccact gagccagagc atcccgctgg
    21241 tgctggcgct ttgtgcctgt gcgttcgcca cctttatcga aatgggcaaa ctgccgttcg
    21301 acttggcgga agccgagcag gagttgcagg aaggtccgct ctctgagtat agcggcagcg
    21361 gctttggcgt catgaaatgg ggcatcagcc tgaaacagct ggtggtgttg cagatgttcg
    21421 tcggggtgtt tattccgtgg ggacaaatgg aaaccttcac cgttggtggg ctgctgctgg
    21481 cgctggtgat tgccatcgtc aaactggtgg ttggcgtcct ggttatcgcg ctgttcgaaa
    21541 acagcatggc ccgtctgcgt cttgatatta ctccgcgcat tacctgggct gggtttggct
    21601 ttgcattttt agcgttcgtc tccttgctgg cggcgtgatt aaagagagtt tgagcatgtc
    21661 tgaagaaaaa ttaggtcaac attatctcgc cgcgctgaat gaggcatttc cgggcgtcgt
    21721 gctggaccac gcctggcaga ccaaagatca gctgaccgtg accgtgaaag tgaactatct
    21781 gccggaagtg gtggagttcc tctactacaa acagggcggt tggctgtcgg tgctgtttgg
    21841 taacgacgaa cgcaaactga atggtcatta cgctgtttac tatgtgctgt cgatggagaa
    21901 gggcaccaag tgctggatta cggttcgcgt cgaagttgac gccaataaac cagaatatcc
    21961 gtccgtgacg ccacgtgttc cggcggcggt gtggggcgag cgtgaagtgc gcgatatgta
    22021 cggtttgatt ccggttgggc tgccggatga gcgccgtctg gtgctgccgg atgactggcc
    22081 ggatgaactt tatcctctgc gtaaagacag catggattat cgtcagcgtc cggcaccgac
    22141 taccgatgct gaaacctacg agttcatcaa cgaactgggc gacaagaaaa acaacgtcgt
    22201 gccgattggt ccactgcacg tcacttctga cgaaccgggt cacttccgtc tgttcgtcga
    22261 tggtgaaaac attatcgacg ccgactaccg cctgttctac gtccatcgcg gcatggaaaa
    22321 actggcggaa acccgtatgg gttacaacga agtgaccttc ctctctgacc gtgtgtgcgg
    22381 gatctgcggc tttgctcaca gcaccgccta taccacgtcg gtggaaaacg cgatgggtat
    22441 tcaggtgcca gaacgcgcgc agatgatccg cgccattctg ctggaggtag aacgcctgca
    22501 ctcgcatctg ctcaatctcg gcctggcctg tcactttacc ggcttcgact ccggctttat
    22561 gcagttcttc cgcgtgcgtg aaacctccat gaaaatggca gagatcctta ccggtgcacg
    22621 taaaacctac ggcctgaacc tgatcggcgg gattcgtcgc gatctgctga aagacgacat
    22681 gatccagacc cgccagctgg cacaacagat gcgtcgtgaa gtgcaggagc tggtggatgt
    22741 gctgctgagc acaccgaaca tggaacaacg caccgtcggc attggtcgtc tggacccgga
    22801 aatcgctcgc gacttcagta acgttggccc gatggttcgt gccagcggtc acgcccgtga
    22861 tacccgtgcc gatcacccgt ttgttggtta tggcctgctg ccaatggaag tccacagcga
    22921 gcagggctgc gacgttattt cccgtctgaa agtgcgtatc aacgaagtct ataccgcgct
    22981 gaatatgatc gattacggtc tggataacct gccgggcggc ccgctgatgg tggaaggctt
    23041 tacctacatt ccgcaccgtt tcgcgctggg ctttgccgaa gcgccgcgcg gcgacgatat
    23101 ccactggagc atgaccggcg acaaccagaa gctgtaccgc tggcgctgcc gtgcggcgac
    23161 ctacgcgaac tggccgaccc tgcgctacat gctgcgcggt aacaccgttt ccgatgcgcc
    23221 gttgattatc ggtagcctgg acccttgcta ctcctgtacc gaccgcatga ccgtagtcga
    23281 tgtacgtaag aagaagagca aagtggtgcc gtacaaagaa ctcgagcgtt acagcattga
    23341 gcgtaaaaac tcgccgctga aataaggaat cgccatgttt acctttatca aaaaagtcat
    23401 caaaaccggc acggcgactt cgtcttatcc gctggagccg attgcggttg ataaaaactt
    23461 ccgtggtaag ccagagcaga acccgcagca gtgcatcggc tgtgcggcct gcgtcaatgc
    23521 ctgcccgtca aacgccttaa cggttgaaac tgacctcgcc acaggagagc tggcgtggca
    23581 gttcaatctt ggacgctgca tcttctgtgg acgctgcgaa gaggtctgcc cgacggcggc
    23641 gatcaaactg tcgcaagaat acgaactggc ggtgtggaag aaagaagact tcctgcaaca
    23701 gtcccgcttc gcgctgtgca actgccgcgt ctgcaatcgt cctttcgccg tccagaaaga
    23761 gatcgactac gccattgcgc tgcttaagca caacggcgac agccgcgcgg aaaaccaccg
    23821 cgaaagcttt gagacttgcc cggaatgtaa gcgccagaaa tgcctggtgc cgtccgaccg
    23881 tattgaactg actcgccata tgaaagaggc catctgatga gcaatttatt aggcccccgt
    23941 gacgccaacg gcattccggt cccgatgacg gtggatgaat ccatcgccag catgaaggcg
    24001 tcgttactga aaaaaatcaa acgttctgcc tacgtttatc gcgtggactg cggcggctgt
    24061 aacggttgcg aaatcgaaat tttcgccacg ctttcgccgc tgttcgatgc agaacgcttc
    24121 ggcattaaag tcgttccgtc accgcgtcat gcggatattt tactgtttac cggcgcggtc
    24181 acccgtgcaa tgcgatcccc tgcgctgcgt gcgtggcagt ccgcgccgga cccgaaaatc
    24241 tgtatctcct acggagcctg cggtaacagc ggcgggatct tccacgatct ctactgcgtg
    24301 tggggcggta cggataaaat cgtcccagtg gatgtttata tccctggctg cccgccaacg
    24361 cctgccgcca cgctgtacgg ctttgcgatg gcgctcggcc tgctggagca gaaaatccac
    24421 gcccgtgggc cgggtgagca ggatgaacaa ccggcggaga tcctgcatgg cgatatggtg
    24481 cagccgctgc gcgtgaaagt ggatcgcgaa gcacgccgcc tggcgggtta tcgttacggt
    24541 cgccagattg ccgatgattt cctgacacag ttagggcagg gcgaagaaca ggttgcacgc
    24601 tggctggaag cggaaaacga tccgcgtctg aacgagattg tcagccatct gaatcatgtt
    24661 gttgaagagg cgcgtatccg atgagtgaaa aggtggtgtt cagtcaactg agccgtaaat
    24721 tcattgatga gaacgatgct acgcccgccg aggcgcagca ggtggtctat tacagcctgg
    24781 cgattggtca ccaccttggg gttatcgatt gcctggaagc ggcgcttacc tgcccgtggg
    24841 atgactatct ggcatggatt gccactctgg aggcgggcag cgaagcccgc cgcaaaatgg
    24901 aaggcgtgcc gaaatatggt gagatcgtca tcgacattaa ccatgtgccg atgctggcca
    24961 acgcattcga taaagcccgg gcagcgcaaa cttcgcagca gaaggaatgg agtacaatgc
    25021 tgttaagtat gctgcatgat attcatcagg aaaacgccat ctatttgatg gtgaggagac
    25081 tgcgtgactg acgttttact ctgtgttggc aatagcatga tgggcgatga tggcgcaggc
    25141 ccgctactgg cggaaaagtg cgctgccgcg ccgaaaggta actgggtagt gattgacggc
    25201 ggtagcgcac cggaaaacga cattgtcgct atccgcgaac tgcgcccgac acggctgctt
    25261 attgtcgacg ccacggatat ggggctaaac cctggcgaga tccgcatcat cgacccggct
    25321 gacatcgccg agatgtttat gatgactacc cataacatgc cgctgaacta tcttatcgac
    25381 cagttgaaag aagatattgg cgaagtgatt ttccttggca tccagccgga tatcgtcggc
    25441 ttttactacc cgatgactca gcagattaaa gatgcggtgg aaacggtgta tcagcggcta
    25501 gaaggctggg aaggaaatgg cggcttcgcg cagttagcgg tggaagaaga gtagtttttc
    25561 agtaaggaat tcagggaatt gaacatcagg acagggatgt gcgctttttt attgagctta
    25621 gtgctgcctg cgcaggctac cagctttacc gaatatctgc caatgagtga cggcgaatat
    25681 gcgcaaaagc gggtgctgaa acctctcctg accatgcctt atgatgccga gcaaacctgg
    25741 cattttagaa aagtgagcgt tgcaggcgta acgcttgaga agatgccaaa tgaagaggat
    25801 tactggcgat taaccgctaa ggaccgtgcc ggtaaatcct ggtttgtgcc tgtgggtgtg
    25861 ctgcaaaata tggcgggtaa tgcgcaattt taccgtgctg acctcgatcg taatggcatt
    25921 caggatttgg ttatctggcg aggtgtttcg ggaaacgggc tggcgccttc ctcgtttttg
    25981 atcctgatga cctttaatca gcaaggtcgc ccgtgtgttt tccaggtcga tggtttttac
    26041 accgcaaacg aaactggcgt tgatgatttg cttgatttac aaggcaatgg tcacacgcaa
    26101 ttgctggata tgcagtttga ttccggttac tggataacca gtttgtatca ggtaaaagag
    26161 gccaaatggc agcgcgttca cggctggttt ggcaagttaa gctaccccgc actcacgcgt
    26221 tttacctata cctccaaccg taaactcgtc attaaacctg tcgccggacg taacccgcaa
    26281 acgaaagatc tggcgctgac acagcgttgt ttaataaagg gtgatgtgct tgatggggtg
    26341 aatcagaact gactttttgc agagtggcta tgaggatttt tgtagtaatt atatcgtgcg
    26401 caagtgttta catattgtga acttggattt gataagctat agtgttccga acatataagg
    26461 atagaactcg cagatgtggc tgtttactta ctataaggaa gttaaaactt aaaaatgcac
    26521 atcatttcta aagcgccatt tgaagagtct gcgcgcaaat atcctaacga cgctctggct
    26581 cttcaggcat tgtatcgggt tattaaggaa acggattttt ctacgccaga agaaatgcga
    26641 acggcgtttc ccaacctcga caattttaaa tatcgcaaca aatggtacgt tctggatgta
    26701 ggtgggaaca acctaagggt gattgcctac ataaactttg ttaataagcg gttctttgtg
    26761 aagcacataa ctaatcacgc cgagtatgac aaactgacac gttactacag agagaacaaa
    26821 gaatgactgc taatgctgta cgcgctgtta aagcaacaag agaactggtc aatgcagtcc
    26881 cctttctggg aggcagtgac tcagaagatg attatcgtga agcactggaa ctggtcgaat
    26941 atttgattga agaagatgac accaatccac ttatcgactt tctgacgagc cgcattgcag
    27001 aatatgaaaa caacaacgaa aagtttgcag agttcgacaa agccgttgcg gcaatgcctg
    27061 ttggtgtggc gttacttcgc acgctgattg atcaacataa tctgacctat gcggatttga
    27121 agaacgaaat cggctctaaa tccctggtca gccagatctt gtctggacaa cgatccttga
    27181 cgatatccca cattaaggca ctttcagccc gatttggcgt taagccggaa tggtttcttt
    27241 gactgatggc agacgctaaa tcgcctgatg cgctacgcct gtcgggcttt gaaagggcgc
    27301 agattcttaa ttttgcgcgc ccttgtttgt cattcaggct gttgtcttta ctactcttaa
    27361 tttatcagta atctcaatca gatatgcttt tgccttttcg cgtgctcgcc cctcaaaaag
    27421 cattatccac tcatgtgtgt cgtcgattgt ttgtaaacgg ccctggagtt ttttgctgaa
    27481 aatcgacgtg agccgataac tgtcgagttg atcaatttgt ttactcgatg tgattttttc
    27541 cccggctgct ttttgaatga taagttcgac gagaccgata acattattat cgtagagaaa
    27601 ttcttcatct ccctgaaatc cgaacaaatc ttcatgctgg cttaagcgtt gaacaattga
    27661 ctcggcatca aaggtcgaat catcttgcag ttgttcctct acccaattaa tcaaatccag
    27721 cccttcgtcg ctttcgagag cctccattcc ccatgaaccc attttgtatc cttacctgtt
    27781 atttcagtcg gaattgtatt atttaataaa ggttcatcgc taatctgcga tgaacctcaa
    27841 aatacaatca tatggatagc tgacaaagtg gtcattgttc acgccgtatc cggcacggtt
    27901 gcttccctta aatcctcccc gttactggca attacttttt tataccacca gaacgacttg
    27961 tcggaaaaaa gtacccagat gtggactggc aacgataaat actgtcatca catgtcatcg
    28021 tcactagtgt cgatgacatc gtttttcctg cattacagag tttaatttcc ttaaataaca
    28081 gcaaattaaa aactggcatg atttgtgaat gtatcggcgc attaactgtc attgctggag
    28141 aatttgatga accgtttcat cattgctgac gcgagtaaat gtattggttg ccgtacctgt
    28201 gaagtagcgt gcgtggtttc tcatcaggaa aatcaggact gtgcatcgct gaccccggaa
    28261 acatttttac cgcgtatcca tgtcattaaa ggtgtgaaca tttccacggc gacagtttgc
    28321 cgtcagtgtg aagatgcacc gtgcgctaac gtctgcccga atggtgctat cagccgtgat
    28381 aaagggtttg ttcatgtcat gcaggaacgt tgtattggtt gcaaaacctg cgttgtggct
    28441 tgcccgtatg gtgcgatgga agtggtggtg cgtccggtga ttcgcaacag cggcgcaggg
    28501 ctgaatgtac gggctgacaa agccgaagcc aataaatgcg acctgtgcaa ccatcgtgaa
    28561 gacggcccgg cgtgtatggc ggcttgcccg acccatgcgc tgatttgtgt cgatcgtaat
    28621 aaacttgaac aactgagcgc agaaaaacgc cgccgcacgg cgctgatgtt ctaaaaatct
    28681 tacagaactg ttctggtatc aaaggaattc ccggaaatat tcgtagaata cgaagcgggc
    28741 ggtatcgccg acctgtgact gccggatgcg acgctgtcgt gtccggcagg gcgggttacg
    28801 caattcatga tggcgggaat aactcaatgg caaaaaacac atcttgcggt gtccaactgc
    28861 gtattcgtgg caaagtgcag ggcgtcggtt ttcgtccgtt tgtctggcaa ctggcacagc
    28921 aattaaatct tcacggcgat gtctgtaatg acggcgatgg cgtagaagtc cggctgctgg
    28981 aagagccgga aacctttctt gttcaattgc atcagcattg tccgccgctg gcgcgtattg
    29041 atagcgtcga gcgtgagccg tttatctggt cacaactgcc cactgagttc accatccgcc
    29101 agagcgcggg tggcgtcatg aatacgcaaa ttgttcccga tgccgcgact tgccctgctt
    29161 gtcttgccga aatgaatacc ccaggcgaac gacgctggcg ttatccgttt atcaactgta
    29221 ctcactgtgg tccgcgtttc accattattc gcgccatgcc ttacgaccgc ccgtttaccg
    29281 tgatggcggc gtttccgctg tgtccggcct gtgacaaaga atatcgcgac ccgctcgatc
    29341 gtcgcttcca cgcccagccg gtggcctgcc cggagtgtgg cccccatctt gaatgggtaa
    29401 gtcatggtga acatgcagaa caagaggcgg cattacaggc agctatcgca cagttaaaaa
    29461 tgggcaacat tgtcgccatc aaagggattg gcggatttca tcttgcctgc gatgcgcgta
    29521 acagtaacgc ggtggcgaca ctgcgggcgc gcaaacatcg cccggcgaaa ccgctggcgg
    29581 tcatgttgcc agtggcagaa ggtttaccag acgctgcgcg ccagttgctt accacgcccg
    29641 ccgcgccgat tgtgctggtg gataaaaaat atgtacctga gctttgtgat gatatcgccc
    29701 ctgaccttaa cgaagtcggg gtaatgttgc ctgcgaaccc gctccagcat ttgctgttac
    29761 aggaactgca atgcccgttg gtgatgacct ccggcaacct gagcggtaaa ccaccggcta
    29821 tcagcaacga acaggcgctg gcggatttgc agggcattgc cgacggattc ttgatacata
    29881 accgcgacat cgtgcagcgg atggatgatt cggtggtgtg cgaaagcggc gaaatgctgc
    29941 gccgttcgcg ggggtatgtg ccggatgcgc tggctttgcc tccgggcttt aaaaatgttc
    30001 cgcctgtgct gtgtctcggt gcggatctga aaaacacctt ctgcctggtg cgcggcgaac
    30061 aagcggtgtt gagtcagcat ctgggcgatt taagtgacga tggcatccag atgcagtggc
    30121 gcgaagcgtt acgcctgatg caaaacatct acgatttcac tccgcaatac gttgtgcatg
    30181 acgcgcatcc gggctatgtc tccagccagt gggcgcgcga aatgaatctg ccgacgcaaa
    30241 cggtactgca tcatcatgcc cacgcagcgg cgtgtctggc agaacacctc tggcctctgg
    30301 atggcggaga tgtcattgcg ttgacgctcg acggtattgg tatgggggag aacggcgctt
    30361 tatggggggg cgagtgcttg cgggtgaact atcgtgaatg ccagcacctg ggcggcttgc
    30421 ccgcggtggc gcttccgggg ggcgatttgg ctgcgaagca gccgtggcga aacctgctgg
    30481 cgcagtgcct gcgctttgtg ccggagtggc agaattactc tgaaacggca agtgtgcaac
    30541 agcaaaactg gagcgtgctg gcgcgggcta ttgagcgtgg aatcaatgcg ccgttggcat
    30601 catcgtgtgg acgtttgttc gatgccgtag cggcggcgct gggctgtgcg ccagccacgt
    30661 taagttatga aggtgaagcg gcttgtgcgc tggaggcgct ggcggcttcg tgccacggcg
    30721 tgacgcatcc ggtgacgatg ccgctggtgg acaatcaact ggatctcgcc actttctggc
    30781 agcagtggct gaactggcag gctccggtta atcaacgcgc gtgggcgttt catgatgcgc
    30841 tggcgcaggg tttggccgcg ttgatgcgtg agcaggccac gatgcgtggt atcactacgc
    30901 tggtatttag cggcggagtt attcataacc gtttgctgcg tgcacgtctg gcgcattatc
    30961 ttgctgattt tacgttgctc ttcccgcaga gttt
//
LOCUS       DADSZD010000037        30455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.037, whole genome shotgun sequence.
ACCESSION   DADSZD010000037 DADSZD010000000
VERSION     DADSZD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.037"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..807
                     /locus_tag="J1Z28_001536"
     CDS             <1..807
                     /locus_tag="J1Z28_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA7716380.1"
                     /translation="GVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPI
                     VRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVV
                     TGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREE
                     IERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIEL
                     PEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            1037..1420
                     /gene="secE"
                     /locus_tag="J1Z28_001537"
     CDS             1037..1420
                     /gene="secE"
                     /locus_tag="J1Z28_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBA7716381.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1422..1967
                     /gene="nusG"
                     /locus_tag="J1Z28_001538"
     CDS             1422..1967
                     /gene="nusG"
                     /locus_tag="J1Z28_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBA7716382.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2126..2554
                     /gene="rplK"
                     /locus_tag="J1Z28_001539"
     CDS             2126..2554
                     /gene="rplK"
                     /locus_tag="J1Z28_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBA7716383.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2558..3262
                     /gene="rplA"
                     /locus_tag="J1Z28_001540"
     CDS             2558..3262
                     /gene="rplA"
                     /locus_tag="J1Z28_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBA7716384.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAE
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     IRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3554..4051
                     /gene="rplJ"
                     /locus_tag="J1Z28_001541"
     CDS             3554..4051
                     /gene="rplJ"
                     /locus_tag="J1Z28_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBA7716385.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4118..4483
                     /gene="rplL"
                     /locus_tag="J1Z28_001542"
     CDS             4118..4483
                     /gene="rplL"
                     /locus_tag="J1Z28_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBA7716386.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4803..8831
                     /gene="rpoB"
                     /locus_tag="J1Z28_001543"
     CDS             4803..8831
                     /gene="rpoB"
                     /locus_tag="J1Z28_001543"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBA7716387.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8908..13131
                     /gene="rpoC"
                     /locus_tag="J1Z28_001544"
     CDS             8908..13131
                     /gene="rpoC"
                     /locus_tag="J1Z28_001544"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBA7716388.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13373..14506)
                     /gene="thiH"
                     /locus_tag="J1Z28_001545"
     CDS             complement(13373..14506)
                     /gene="thiH"
                     /locus_tag="J1Z28_001545"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBA7716389.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYTVAEHLLWLQQHYWQSRYSVSFPRLRPCTGSIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(14503..15273)
                     /gene="thiG"
                     /locus_tag="J1Z28_001546"
     CDS             complement(14503..15273)
                     /gene="thiG"
                     /locus_tag="J1Z28_001546"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBA7716390.1"
                     /translation="MLHIADKTFDSHLFTGTGKFASSQLMVESIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFIVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15275..15475)
                     /gene="thiS"
                     /locus_tag="J1Z28_001547"
     CDS             complement(15275..15475)
                     /gene="thiS"
                     /locus_tag="J1Z28_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBA7716391.1"
                     /translation="MQILFNDQPMQCVAGLTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15459..16214)
                     /gene="thiF"
                     /locus_tag="J1Z28_001548"
     CDS             complement(15459..16214)
                     /gene="thiF"
                     /locus_tag="J1Z28_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBA7716392.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGETLKDAVAQADVVLDCTDNMATRQEINATCVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLTLRRANGCPVCGGCNADPV"
     gene            complement(16207..16842)
                     /gene="thiE"
                     /locus_tag="J1Z28_001549"
     CDS             complement(16207..16842)
                     /gene="thiE"
                     /locus_tag="J1Z28_001549"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBA7716393.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSTIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16842..18737)
                     /gene="thiC"
                     /locus_tag="J1Z28_001550"
     CDS             complement(16842..18737)
                     /gene="thiC"
                     /locus_tag="J1Z28_001550"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBA7716394.1"
                     /translation="MSVTKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18818..18918)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18970..19446)
                     /gene="rsd"
                     /locus_tag="J1Z28_001551"
     CDS             complement(18970..19446)
                     /gene="rsd"
                     /locus_tag="J1Z28_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBA7716395.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19541..20314
                     /gene="nudC"
                     /locus_tag="J1Z28_001552"
     CDS             19541..20314
                     /gene="nudC"
                     /locus_tag="J1Z28_001552"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBA7716396.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20354..21418
                     /gene="hemE"
                     /locus_tag="J1Z28_001553"
     CDS             20354..21418
                     /gene="hemE"
                     /locus_tag="J1Z28_001553"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBA7716397.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVL
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21428..22099
                     /gene="nfi"
                     /locus_tag="J1Z28_001554"
     CDS             21428..22099
                     /gene="nfi"
                     /locus_tag="J1Z28_001554"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBA7716398.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22142..22732
                     /locus_tag="J1Z28_001555"
     CDS             22142..22732
                     /locus_tag="J1Z28_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBA7716399.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22919..23191
                     /gene="hupA"
                     /locus_tag="J1Z28_001556"
     CDS             22919..23191
                     /gene="hupA"
                     /locus_tag="J1Z28_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBA7716400.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23204..23899
                     /locus_tag="J1Z28_001557"
     CDS             23204..23899
                     /locus_tag="J1Z28_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBA7716401.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRNGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23901..24320)
                     /gene="zraP"
                     /locus_tag="J1Z28_001558"
     CDS             complement(23901..24320)
                     /gene="zraP"
                     /locus_tag="J1Z28_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBA7716402.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLATNPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGGCGGGGHMGMGHW"
     gene            24558..25934
                     /gene="zraS"
                     /locus_tag="J1Z28_001559"
     CDS             24558..25934
                     /gene="zraS"
                     /locus_tag="J1Z28_001559"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBA7716403.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSGQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25931..27256
                     /gene="zraR"
                     /locus_tag="J1Z28_001560"
     CDS             25931..27256
                     /gene="zraR"
                     /locus_tag="J1Z28_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBA7716404.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLTNHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27253..28542)
                     /gene="purD"
                     /locus_tag="J1Z28_001561"
     CDS             complement(27253..28542)
                     /gene="purD"
                     /locus_tag="J1Z28_001561"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBA7716405.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVSDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28554..30143)
                     /gene="purH"
                     /locus_tag="J1Z28_001562"
     CDS             complement(28554..30143)
                     /gene="purH"
                     /locus_tag="J1Z28_001562"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBA7716406.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIINEM
                     DANEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7124 a         7882 c         8402 g         7047 t
ORIGIN      
        1 ggcgttccgt acatcatcgt gttcctgaac aaatgcgaca tggttgatga cgaagagctg
       61 ctggaactgg ttgaaatgga agttcgtgaa cttctgtctc agtacgactt cccgggcgac
      121 gacactccga tcgttcgtgg ttctgctctg aaagcgctgg aaggcgacgc agagtgggaa
      181 gcgaaaatcc tggaactggc tggcttcctg gattcttaca ttccggaacc agagcgtgcg
      241 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
      301 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      361 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      421 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      481 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      541 tctgaagtgt acattctgtc caaagatgaa ggcggccgtc atactccgtt cttcaaaggc
      601 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      661 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      721 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccgtac cgttggcgcg
      781 ggcgttgtag caaaagttct gagctaattg ccgataacat ttgacgcaat gcgcactaaa
      841 agggcatcat ttgatgccct ttttgcacgc tttcgtacca gaacctggct catcagtgat
      901 tttctttgtc ataatcattg ctgagacagg ctctgttgag ggcgtataat ccgaaaagct
      961 aatacgcgtt tcgatttggt ttgcctcgcg atcgcggggt gaaaatgttt gtagaatact
     1021 tctgacaggt tggtttatga gtgcgaatac cgaagctcaa ggaagcgggc gcggcctgga
     1081 agcgatgaag tgggtcgttg tggtggcatt gctcctggta gcgattgtcg gcaactacct
     1141 ttatcgcgac attatgctgc cgctgcgtgc gctggccgta gtaattctga ttgctgcagc
     1201 gggtggtgtc gcgctgttaa cgacaaaagg taaagctacc gttgcttttg cccgtgaagc
     1261 gcgtaccgaa gtccgtaagg tcatttggcc gactcgccag gaaacattgc acaccacgct
     1321 gattgtggct gcggttaccg cagtaatgtc actgatcctg tggggactgg atggtattct
     1381 ggttcgcctg gtatccttta tcactggcct gaggttctga gatgtctgaa gctcctaaaa
     1441 agcgctggta cgtcgttcag gcgttttccg gttttgaagg ccgcgtagca acgtcgctgc
     1501 gtgagcatat caaattacac aacatggaag agttgtttgg tgaagtcatg gtaccaaccg
     1561 aagaagtggt tgaaatccgt ggcggtcagc gtcgcaaaag cgaacgtaaa ttcttccctg
     1621 gctacgtcct cgttcagatg gtgatgaacg acgcgagctg gcacctggtg cgcagcgtac
     1681 cgcgtgtgat gggcttcatc ggcggtactt ccgatcgtcc tgcgccaatc agcgataaag
     1741 aagtcgatgc gattatgaac cgcctgcagc aggttggtga taagccgcgt ccgaaaacgt
     1801 tgtttgaacc gggtgaaatg gtccgtgtta atgatggtcc gttcgctgac ttcaacggcg
     1861 ttgttgaaga agttgattac gagaaatctc gtctgaaagt gtctgtttct atcttcggtc
     1921 gtgcgacccc tgtagagctg gacttcagcc aggttgaaaa agcttaaccc agcgatcaaa
     1981 aaagcggcga tttaatcgtt gcacaaggcg cgagattgga atacaatttc gcgccttttg
     2041 tttttatggg ccttgcccgt aaaacgattt tttatatcac ggggagcctc tcagaggcgt
     2101 tattacccaa cttgaggaat ttataatggc taagaaagta caagcctatg tcaagctgca
     2161 ggttgcagct ggtatggcta acccgagtcc gccagtaggt ccggctctgg gtcagcaggg
     2221 cgtaaacatc atggaattct gcaaagcgtt caacgcaaaa actgattcca tcgaaaaagg
     2281 tctgccgatt ccggtagtaa tcactgttta cgctgaccgt tctttcactt tcgttaccaa
     2341 gactccgccg gcagcagttc tgctgaaaaa agcggctggt atcaagtctg gttccggtaa
     2401 gccgaacaaa gacaaagtgg gtaaaatttc ccgcgctcag ctgcaggaaa tcgcgcagac
     2461 caaagctgcc gacatgactg gtgccgacat tgaagcgatg actcgctcca tcgaaggtac
     2521 tgcacgttcc atgggcctgg tagtggagga ctaagaaatg gctaaactga ccaagcgcat
     2581 gcgtgttatc cgcgagaaag ttgatgcaac caaacagtac gacatcaacg aagctatcgc
     2641 tctgctgaaa gagctggcga ctgctaaatt cgtagaaagc gtggacgtag ctgttaacct
     2701 cggcatcgac gctcgtaaat ctgaccagaa cgtacgtggt gcaactgtac tgccgcacgg
     2761 tactggccgt tccgttcgcg tagccgtatt tacccagggt gcaaatgctg aagctgctaa
     2821 agctgcaggt gctgaactgg taggtatgga agatctggct gaacagatta agaaaggcga
     2881 aatgaacttt gacgttgtta ttgcttctcc ggatgcaatg cgcgttgttg gccagctggg
     2941 ccaggttctg gggccgcgcg gcctgatgcc aaacccgaaa gtgggtactg taacgccgaa
     3001 cgttgctgaa gcggttaaaa acgctaaagc tggccagatt cgttaccgta acgacaaaaa
     3061 cggcatcatc cacaccacca tcggtaaagt agactttgac gctgacaaac tgaaagaaaa
     3121 cctggaagct ctgctggttg cgctgaaaaa agcaaaaccg actcaggcga aaggcgtgta
     3181 catcaagaaa gttagcatct ctaccaccat gggtgcaggt gttgcagttg accaggctgg
     3241 cctgagcgct tctgtaaact aatgccttta cgtgggcggt gattttgtct acaatcttac
     3301 ccccacgtat gctaacgaaa gttagcagct aaaaagattt gttcgttgga gcctggccta
     3361 tccaggcctc cgtcgaagac cgcaggagtt tcgcaagaaa cttaatcccc tgcgtagacg
     3421 gtgacagaac gctaagatta ttcttttata ttctggcttg tttctgctca ccgtaattaa
     3481 gacgctctct ccgtttggag gagtgaagtg agttccagag attttctctg gcaaacatcc
     3541 aggagcaaag ctaatggctt taaatcttca agacaaacaa gcgattgttg ctgaagtcag
     3601 cgaagtagcc aaaggcgcgc tgtctgcagt agttgcggat tcccgtggcg taactgtaga
     3661 taaaatgact gaactgcgta aagcaggtcg cgaagctggc gtatacatgc gtgttgttcg
     3721 taacaccctg ctgcgccgtg ctgttgaagg tactccgttc gagtgcctga aagacgcgtt
     3781 tgttggtccg accctgattg catactctat ggaacacccg ggcgctgctg ctcgtctgtt
     3841 caaagagttc gcgaaagcga atgcaaaatt tgaggtcaaa gccgctgcct ttgaaggtga
     3901 gctgatcccg gcgtctcaga tcgaccgcct ggcaactctg ccgacctacg aagaagcaat
     3961 tgcacgcctg atggcaacca tgaaagaagc ttcggctggc aaactggttc gtactctggc
     4021 tgctgtacgc gatgcgaaag aagctgctta atcgcagtta tctttttaac gcattcgctt
     4081 acgtataaac ttattctgat attcaggaac aatttaaatg tctatcacta aagatcaaat
     4141 cattgaagca gttgcagcta tgtctgtaat ggacgttgta gaactgatct ctgcaatgga
     4201 agaaaaattc ggtgtttccg ctgctgctgc tgtagctgta gctgctggcc cggttgaagc
     4261 tgctgaagaa aaaactgaat tcgacgtaat tctgaaagct gctggcgcta acaaagttgc
     4321 tgttatcaaa gcagtacgtg gcgcaactgg cctgggtctg aaagaagcta aagacttggt
     4381 tgaatctgca ccggctgctc tgaaagaagg cgtgagcaaa gacgacgcag aagcactgaa
     4441 aaaagctctg gaagaagctg gcgctgaagt tgaagttaaa taagccaacc cttccggttg
     4501 cagcctgaga aatcaggctg atggctggtg actttttagt caccagcctt tttgcgctgt
     4561 aaggcgccag tagcgtttca cactgtttga ctactgctgt gcctttcaat gcttgtttct
     4621 atcgacgact taatatactg cgacaggacg cccgttctgt gtaaatcgca atgaaatggt
     4681 ttaagcgtga tagcaacagg cattgcggaa agtgttccat tttccggtca acaaaatagt
     4741 gttgcacaaa ctgtccgctc aatggacaga tgggtcgact tgtcagcgag ctgaggaacc
     4801 ctatggttta ctcctatacc gagaaaaaac gtattcgtaa ggattttggt aaacgtccac
     4861 aagttctgga tgtaccttat ctcctttcta tccagcttga ctcgtttcag aaatttatcg
     4921 agcaagatcc tgaagggcag tatggtctgg aagctgcttt ccgttccgta ttcccgattc
     4981 agagctacag cggtaattcc gagctgcaat acgtcagcta ccgccttggc gaaccggtgt
     5041 ttgacgtcca ggaatgtcaa atccgtggcg tgacctattc cgcaccgctg cgcgttaaac
     5101 tgcgtctggt gatctatgag cgcgaagcgc cggaaggcac cgtaaaagac attaaagaac
     5161 aagaagtcta catgggcgaa attccgctca tgacggacaa cggtaccttt gttatcaacg
     5221 gtactgagcg tgttatcgtt tcccagctgc accgtagtcc gggggtcttt tttgactccg
     5281 acaaaggtaa aacccactct tcgggtaagg tgctgtataa cgcgcgcatc atcccttacc
     5341 gtggttcctg gctggacttc gaattcgatc cgaaggacaa cctgttcgta cgtatcgacc
     5401 gtcgccgcaa actacctgcg acaatcattc tgcgtgccct gaactacacc acagagcaga
     5461 tcctcgacct gttctttgaa aaagttatct ttgaaatccg tgataacaag ttgcagatgg
     5521 aactggtgcc ggaacgcctg cgtggtgaaa ccgcatcttt tgacatcgaa gctaacggta
     5581 aagtgtacgt agaaaaaggc cgccgcatta ctgcgcgcca tattcgccag ctggaaaaag
     5641 acgacgtcaa gctgatcgaa gtcccggttg agtacatcgc aggtaaagtg gttgctaaag
     5701 actacattga tgagtctacc ggcgagctga tctgcgcagc gaacatggag ctgagcctgg
     5761 atctgctggc taagctgagc cagtctggtc ataagcgtat cgaaacgctg ttcaccaacg
     5821 atctggatca cggcccatat atctctgaaa ccttacgtgt cgacccaact aacgaccgtc
     5881 tgagcgcact ggtagaaatc taccgtatga tgcgccctgg cgagccgccg actcgtgaag
     5941 ctgctgaaag cctgttcgag aacctgttct tctccgaaga ccgctatgac ttgtctgcgg
     6001 ttggtcgtat gaagttcaac cgttctctgc tgcgcgaaga aatcgaaggt tccggtatcc
     6061 tgagcaaaga cgacatcatt gatgttatga aaaagctcat cgatatccgt aacggtaaag
     6121 gcgaagtcga tgatatcgac cacctcggca accgtcgtat ccgttctgtt ggcgaaatgg
     6181 cggaaaacca gttccgcgtt ggcctggtac gtgtagagcg tgcggtgaaa gagcgtctgt
     6241 ctctgggcga tctggatacc ctgatgcctc aggatatgat caacgccaag ccgatttccg
     6301 cagcagtgaa agagttcttc ggttccagcc agctgtctca gtttatggac cagaacaacc
     6361 cgctgtctga gattacgcac aaacgtcgta tctcggcact cggcccaggc ggtctgaccc
     6421 gtgaacgtgc aggcttcgaa gttcgagacg tacacccgac tcactacggt cgcgtatgtc
     6481 caatcgaaac ccctgaaggt ccgaacatcg gtctgatcaa ctccctgtcc gtgtacgcac
     6541 agactaacga atacggcttc cttgagactc cgtatcgtaa agtgaccgac ggtgttgtaa
     6601 ctgacgaaat tcactatctg tctgctatcg aagaaggcaa ctacgttatc gcccaggcga
     6661 actccaacct ggatgaagaa ggccacttcg tagaagacct ggtaacttgc cgtagcaaag
     6721 gcgaatccag cttgttcagc cgtgaccagg ttgactacat ggacgtatcc acccagcagg
     6781 tggtatccgt cggtgcgtcc ctgatcccgt tcctggaaca cgatgacgcc aaccgtgcat
     6841 tgatgggtgc gaacatgcaa cgtcaggccg ttccgactct gcgcgctgat aagccgctgg
     6901 ttggtacggg tatggaacgt gctgttgccg ttgactccgg tgtaactgcg gttgctaaac
     6961 gtggtggtgt cgttcagtac gtggatgctt cccgtatcgt tatcaaagtt aacgaagacg
     7021 agatgtatcc gggtgaagca ggtatcgaca tctacaacct gaccaaatac acccgttcta
     7081 accagaacac ctgtattaac cagatgccgt gtgtgtctct gggtgaaccg gttgaacgtg
     7141 gcgacgtgct ggcagacggt ccgtccaccg acctcggtga actggcgctt ggtcagaaca
     7201 tgcgcgtagc gttcatgccg tggaatggtt acaacttcga agactccatc ctcgtatccg
     7261 agcgtgttgt tcaggaagac cgtttcacca ccatccacat tcaggaactg gcgtgtgtgt
     7321 cccgtgacac caagctgggg ccagaagaga tcaccgctga catcccgaac gtgggtgaag
     7381 ctgcgctctc caaactggat gaatccggta tcgtttacat tggtgcggaa gtgaccggtg
     7441 gcgacattct ggttggtaag gtaacgccga aaggtgaaac tcagctgacc ccagaagaaa
     7501 aactgctgcg tgcgatcttc ggtgagaaag cgtctgacgt taaagactct tctctgcgcg
     7561 taccaaacgg tgtatccggt acggttatcg acgttcaggt ctttactcgc gatggcgtag
     7621 aaaaagacaa acgtgcgctg gaaatcgaag aaatgcagct caaacaggcg aagaaagacc
     7681 tgtctgaaga actgcagatc ctcgaagcgg gtctgttcag ccgtatccgt gctgtgctgg
     7741 tagccggtgg cgttgaagct gagaagctcg acaaactgcc gcgcgatcgc tggctggagc
     7801 tgggcctgac cgacgaagag aaacaaaatc agctggaaca gctggctgag cagtatgacg
     7861 aactgaaaca cgagttcgag aagaaactcg aagcgaaacg ccgcaaaatc acccagggcg
     7921 acgatctggc accgggcgtg ctgaagattg ttaaggtata tctggcggtt aaacgccgta
     7981 tccagcctgg tgacaagatg gcaggccgtc atggtaacaa gggtgtaatt tctaagatca
     8041 acccgatcga agatatgcct tacgatgaaa acggtactcc ggtagacatc gtactgaacc
     8101 cgctgggcgt accgtctcgt atgaacattg gtcagatcct cgaaacccac ctgggtatgg
     8161 ctgcgaaagg tatcggcgac aagatcaacg ccatgctgaa acagcagcaa gaagtcgcga
     8221 aactgcgcga attcatccag cgtgcgtacg atctgggcgc tgacgttcgt cagaaagttg
     8281 acctgagtac cttcagcgat gaagaagtta tgcgtctggc tgaaaacctg cgcaaaggta
     8341 tgccaatcgc aacgccggtc ttcgacggtg cgaaagaagc agaaattaaa gagctgctga
     8401 aacttggcga cctgccgact tctggtcaga tccgcctgta cgacggtcgc actggtgaac
     8461 agttcgagcg tccggtaacc gttggttaca tgtacatgct gaaactgaac cacctggtcg
     8521 acgacaagat gcacgcgcgt tccaccggtt cttatagcct ggttactcag cagccgctgg
     8581 gtggtaaggc acagttcggt ggtcagcgtt tcggggagat ggaagtgtgg gcgctggaag
     8641 catatggcgc agcatacacc ctgcaggaaa tgctcaccgt taagtctgat gacgtgaacg
     8701 gtcgtactaa gatgtataaa aacatcgtgg acggcaacca tcagatggag ccgggcatgc
     8761 cagaatcctt caacgtattg ttgaaagaga ttcgttcgct gggtatcaac atcgaactgg
     8821 aagacgagta attctcgctc aaacaggtca ctgctgtcgg ggtaaaaccc ggcagcggat
     8881 tgtgctaact ccgacgggag caaatccgtg aaagatttat taaagtttct gaaagcgcag
     8941 actaaaaccg aagagtttga tgcgatcaaa attgctctgg cttcgccaga catgatccgt
     9001 tcatggtctt tcggtgaagt taaaaagccg gaaaccatca actaccgtac gttcaaacca
     9061 gaacgtgacg gccttttctg cgcccgtatc tttgggccgg taaaagatta cgagtgcctg
     9121 tgcggtaagt acaagcgcct gaaacaccgt ggcgtcatct gtgagaagtg cggcgttgaa
     9181 gtgacccaga ctaaagtacg ccgtgagcgt atgggccaca tcgaactggc ttccccgact
     9241 gcgcacatct ggttcctgaa atcgctgccg tcccgtatcg gcctgctgct cgatatgccg
     9301 ctgcgcgata tcgaacgtgt actgtacttc gaatcctatg tggttatcga aggtggtatg
     9361 accaacctgg agcgtcagca gatcctgact gaagagcagt atctggacgc gctggaagag
     9421 ttcggtgacg aattcgacgc gaagatgggt gcggaagcaa tccaggccct gctgaagagc
     9481 atggatctgg agcaagagtg cgaacagctg cgtgaagagc tgaacgaaac caactccgaa
     9541 accaagcgta aaaagctgac caagcgtatc aaactgctgg aagcgttcgt tcagtctggt
     9601 aacaaaccag agtggatgat cctgaccgtt ctgccggtac tgccgccaga tctgcgtccg
     9661 ctggttccgc tggatggtgg ccgtttcgcg acttctgacc tgaacgatct gtatcgtcgc
     9721 gtcattaacc gtaacaaccg tctgaaacga ctgctggacc tggctgcgcc ggatatcatc
     9781 gtacgtaacg aaaaacgtat gctgcaggaa gcggtagacg ccctgctgga taacggtcgt
     9841 cgcggtcgtg caatcaccgg ttctaacaag cgtcctctga aatctttggc cgacatgatc
     9901 aaaggtaaac agggtcgttt ccgtcagaac ctgctcggta agcgtgttga ctactccggt
     9961 cgttctgtaa tcaccgtagg tccatacctg cgtctgcatc agtgcggtct gccgaagaaa
    10021 atggcgctgg agctgttcaa acctttcatc tacggcaagc tggaactgcg tggtcttgct
    10081 accaccatca aagctgcgaa gaaaatggtt gagcgcgaag aagctgtcgt ttgggatatc
    10141 ctggacgaag ttatccgcga acacccggta ctgctgaacc gtgcaccgac tctgcaccgt
    10201 ctgggtatcc aggcatttga accggtactg atcgaaggta aagctatcca gctgcacccg
    10261 ctggtttgtg cggcatataa cgccgacttc gatggtgacc agatggccgt tcacgtaccg
    10321 ctgacgctgg aagcccagct cgaagcgcgt gcgctgatga tgtctaccaa caacatcctg
    10381 tccccggcga acggcgaacc aatcatcgtt ccgtctcagg acgttgtact gggtctgtac
    10441 tacatgaccc gcgactgtgt taacgccaaa ggcgaaggca tggtgctgac tggcccgaaa
    10501 gaagcagaac gtctgtatcg ctctggtctg gcttctctgc atgcgcgcgt taaagtgcgt
    10561 atcaccgagt atgaaaaaga tgctaacggt gaattagtgg cgaaaaccag cctgaaagac
    10621 acgactgttg gccgtgccat tctgtggatg attgtaccga aaggtctgcc ttacaccatc
    10681 gtcaaccagg cgctgggtaa aaaagcaatc tccaaaatgc tgaacacctg ctaccgcatt
    10741 ctcggtctga aaccgaccgt tatttttgcg gaccagatca tgtataccgg ctttgcatat
    10801 gcagcgcgtt ctggtgcatc tgttggtatc gatgacatgg tcatcccgga gaagaaacac
    10861 gaaatcatct ccgaggcaga agcagaagtt gctgaaattc aggagcagtt ccagtctggt
    10921 ctggtaactg cgggcgaacg ctacaacaaa gttatcgata tctgggctgc ggcgaacgat
    10981 cgtgtatcca aagcgatgat ggataacctg caaactgaaa ccgttattaa ccgtgacggt
    11041 caggaagaga agcaggtttc cttcaacagc atctacatga tggccgactc cggtgcgcgt
    11101 ggttctgcgg cacagattcg tcagcttgct ggtatgcgtg gtctgatggc gaagccggat
    11161 ggctccatca tcgaaacacc aatcaccgcg aacttccgtg aaggtctgaa cgtactccag
    11221 tacttcatct ccacccacgg tgctcgtaaa ggtctggcgg ataccgcact gaaaactgcg
    11281 aactccggtt acctgactcg tcgtctggtt gacgtggcgc aggacctggt ggttaccgaa
    11341 gacgattgtg gtacccatga aggtatcatg atgactccgg ttatcgaggg tggtgacgtt
    11401 aaagagccgc tgcgcgatcg cgtattgggt cgtgtaactg ctgaagacgt tctgaagccg
    11461 ggtactgctg atatcctcgt tccgcgcaac acgctgctgc acgaacagtg gtgtgacctg
    11521 ctggaagaga actctgtcga cgcggttaaa gtacgttctg ttgtatcttg tgacaccgac
    11581 tttggtgtat gtgcgcactg ctacggtcgt gacctggcgc gtggccacat catcaacaaa
    11641 ggtgaagcaa tcggtgttat cgcggcacag tccatcggtg aaccgggtac acagctgacc
    11701 atgcgtacgt tccacatcgg tggtgcggca tctcgtgcgg ctgctgaatc cagcatccag
    11761 gtgaaaaaca aaggtagcat caagctcagc aacgtgaagt cggttgtgaa ctccagcggt
    11821 aaactggtta tcacttcccg taacaccgaa ctgaaactga tcgacgaatt cggtcgtacc
    11881 aaagaaagct acaaagtacc ttacggtgcg gtactggcga aaggcgatgg cgaacaggtt
    11941 gctggcggcg aaaccgttgc aaactgggac ccgcacacca tgccggttat caccgaagta
    12001 agcggttttg tacgctttac tgacatgatc gacggccaga ccattactcg tcagaccgac
    12061 gaattgacag gtctgtcttc gctggtggtt ctggattctg cagaacgtac cgcaggtggt
    12121 aaagatctgc gtccggcact gaaaatcgtt gatgctcagg gtaacgacgt tctgatcccg
    12181 ggtaccgata tgcctgcgca gtacttcctg ccgggtaaag cgattgttca gctggaagat
    12241 ggcgtacaga tcagctctgg tgacaccctg gcgcgtattc cgcaggaatc cggcggtacc
    12301 aaggacatca ccggtggtct gccgcgcgtt gcggacctgt tcgaagcacg tcgtccgaaa
    12361 gagccggcaa tcctggctga aatcagcggt atcgtttcct tcggtaaaga aaccaaaggt
    12421 aaacgtcgtc tggttatcac cccggtagac ggtagcgatc cgtacgaaga gatgattccg
    12481 aaatggcgtc agctcaacgt gttcgaaggt gaacgtgtag aacgtggtga cgtaatttcc
    12541 gacggtccgg aagcgccgca cgacattctg cgtctgcgtg gtgttcatgc tgtgactcgt
    12601 tacatcgtta acgaagtaca ggacgtatac cgtctgcagg gcgttaagat taacgataaa
    12661 cacatcgaag ttatcgttcg tcagatgctg cgtaaagcta ccatcgttaa cgcgggcagc
    12721 tccgacttcc tggaaggtga acaggttgaa tactctcgcg tcaagatcgc aaaccgcgaa
    12781 ctggaagcga acggcaaagt gggtgcaact tactcccgcg atctgctggg tatcaccaaa
    12841 gcgtctctgg caaccgagtc cttcatctcc gcggcatcgt tccaggagac cactcgcgtg
    12901 ctgaccgaag cagccgttgc gggcaaacgc gacgaactgc gcggcctgaa agagaacgtt
    12961 atcgtgggtc gtctgatccc ggcaggtacc ggttatgcgt accaccagga tcgtatgcgt
    13021 cgccgtgctg cgggtgaagc tccggctgca ccgcaggtga ctgcagaaga cgcatctgcc
    13081 agcctggcag aactgctgaa cgcaggtctg ggcggttctg ataacgagta atcgttaatc
    13141 cgcaaataac gtaaaaaccc gcttcggcgg gtttttttat gggtgttatt cagagggcaa
    13201 agtcttcacg ggaaatatta agaaaggcag ggcgaaacga ggaagaagct tttcgccctg
    13261 gacaacattc ctgttgacgg gactaccata aaaaaacaca acgctttcaa caaccttgcg
    13321 tttttcatct tccggagctg ctcggaataa atttacgttc ttacattctg tctcatggcc
    13381 tttgcgatgg gcgtcccaga tagctgtccc agtctttcca taccggctgc aaaccctgag
    13441 cggttaacgc ggcagcaacc gcttccggtc tgcgatcgtc gtgcggtgag aactgttcca
    13501 gctcggggtg gttgtcggca tagccacctg gctgcgtttt cgaaaaagcg ctgacgttat
    13561 taattgccag cggaattacg cgatcgcgaa accacggtga ttcccgcgtg gagagtgaca
    13621 gttcaatctc cggtgcaagc agccggaaag cgcagatggc ttgcactaac tggcgttcat
    13681 ccataatcga cgcaggctca atgctgccag tacatggacg caggcgtggg aaggagacag
    13741 agtagcggct ttgccagtaa tgctgttgca gccatagcaa atgttcggca accgtatagc
    13801 agtcaactcg ccagttgtcg gaaaggccga ttagcgcgcc gaggcctatc ttatcaatcc
    13861 ccgcacgccc tagccgatcc ggcgtttcca gccgccagaa gaagtcctgt tttttacctt
    13921 tcagatgatg gcgggcataa gtcgcctcgt gatatgtctc ctgataaacc atcacgccat
    13981 ccagacctag ttgctttaac tcggcgtatt ccgtctccgc cagcggttgc acttccatct
    14041 gtagtgaaga gaactgttca cgcagggcag ggagatgacg acgaaagtaa tccatcccca
    14101 ctttcgcctg atgttcacca gtgactaata gcagatgttc aaagcccatc tcccgtatag
    14161 cggcgctttc cctggcaata tccgcttcat ccagcgtttt gcgcttgatg cggttgctca
    14221 tagaaaatcc gcaataagta cagtcgttag cgcaaagatt ggaaagataa agcgggacgt
    14281 agaagctgac gacgttgcca aatcgctgac gagtcagacg ttgcgctcgc tgggccagtg
    14341 gttccagata gccactggcg gcgggtgata gcaatgccat catgtcgtcg cgggtgagtt
    14401 gcgaggcatt tagcgcccgc tctacgtcag cagccgtttt gccgttgata cgcaggcgga
    14461 tgtcatccca gtccagttgt cgccagcgat cgctgaaggt tttcatgccg atgcctccag
    14521 aaatccagtc agcgggctgg tggcatgagc aaaatgactg cggctgcccg gtccggactg
    14581 acgtgccagc aggcctgctt ctaccgccag acgaaatgcc ttcgccatgt tgacgggatc
    14641 gtccgcgacg gcaatcgccg tattcactaa caccgcgtcg gcccccattt ccagcgcctg
    14701 cgcggcatgg ctgggaacgc cgatgccagc atcgacaacc accggaacgg tggcctgctg
    14761 gataataatc tccagcatgg cgcgggtttc cagtccctga ttcgagccaa tcggcgcgcc
    14821 gagcggcatc accgctgcac agccgacttc ttccagacgt ttacacaata ccggatcggc
    14881 cccgcagtaa ggcagcacga taaatccctg ttgtaccagc gtttcggcgg ctttcagggt
    14941 ttcgatggga tcgggcaaca gccagcgggc gtcagggtga atctccaatt ttagccagtt
    15001 tgtgcctaac gcttcacgtg ccagatgggc ggcgaaaatg gcttcttccg cggttttcgc
    15061 cccggaagta ttcggcagca gggtcacacc cgccgcgata agcggttcga ggatagcgtc
    15121 gttgtgctgg cgcaagtcga cacgtttcat cgccagcgtc accagttggc tgccggaagc
    15181 gcgaatagac tccaccatca gttgtgaaga ggcgaatttt ccggtgccag taaacagatg
    15241 tgaatcaaac gttttgtccg caatatgtaa catctcaacc ccctgcaata acctgaaaaa
    15301 gcaggatctg gtcgccatcc tgcacgatat gttgcgccca ttgttcccgc gggacgattt
    15361 gctgattaat cgccagtgcc gcacctgctt gttgttgatc gagttgctcc agcagttcgt
    15421 gaacagttag tccggcgaca cactgcatcg gttgatcgtt aaacaggatc tgcattgcat
    15481 cctccacata ccgggcaacc gttggcgcgg cgcaacgtca ggctgcgcca ctggctcgat
    15541 ttaccgtcga acagtcggag ttctcccgca ggcgtctcta taccgcttaa taacttaatc
    15601 gcttccagtg cctgtaaagt gcccataacc ccgaccaccg ggccaaccac gcccgccgtg
    15661 cggcagttgc gttctggctc ctggttatct ggccacaggc agcggtaaca tccctgctcc
    15721 cagggcggcg tcagtaccat caactgaccg ccaaatccga ccgcgctggc ggtgataagc
    15781 ggcgtgttga gtgccacgca ggtggcatta atctcctggc gagtcgccat attgtcagta
    15841 cagtcgagca ccacatcggc ttgtgcaacc gcatctttta acgtctcacc cgttaatcgt
    15901 tgttgtaatg ccatcagttg aatgtcggga ttcaactgtg tcagtcgctg ttggctgacc
    15961 tgtgatttcg ggcgatcgat atcttcagtg gtaaagagga tttgtcgttg cagattgctt
    16021 aaatgcacat cgtcgtcatc tgccagtacc agcgtcccga cgccagcccc cgccaggtat
    16081 agcgcagcag gtgtacccag cccgccaaga ccaataatca gcacctggct gtcgagcagt
    16141 ttttgctgcc cgtccagagc gatatcgtcg agcaggattt ggcggctata acgcataaag
    16201 tcacggtcat tcatcgccaa ctcctgcaat ttccagcagc tgtgccgttg ccaaacgcca
    16261 gtctgcggct tgagtaatgg cgctgacgac ggcgatactg ccgacacccg ttgctatcac
    16321 cgcaggcgcg cgcgccagac tgataccgcc aatcgccacg gtgggataat ccgccaggcg
    16381 ctcaacatgc cgtgccagct gttccagtcc ctgcggtgca gaaggcatct gtttggtttg
    16441 tgtcggaaac acatgtccca gcgcgatata agaaggacgt gccgccagcg cgacgtcgat
    16501 ctccatatcg tcatgtgtcg aaacgcccag tcgcagacct gccgcgcgga tggtgctgag
    16561 atcggtggct tgcaaatctt cctgccccag atggacgccg tatgcctgat gcttgatcgc
    16621 cagtcgccag taatcattaa taaacaatcg cgcgttatag cggcggccca gcgcaattgc
    16681 cgccacgaca tcggcttcca cctcctcatc gcgctgatct ttgatgcgta gctggagagt
    16741 acgtacgcct gcatccaaca gacgttcgat ccactgtacg ctgtcaacca ccgggtacag
    16801 tcctaaacga aagggtacag gaggaaactc aggctgatac atcacgcttc ctccttacgc
    16861 aggtagattt ctccgcctct ggcgcggaag ttctccgaca tatccgccat tcccacttca
    16921 atggtttgcg cggcggcgta atcacgcact tcctggctga ttttcatcga gcagaatttc
    16981 ggtccacaca tggagcaaaa atgggcgact ttaccggact cttgcggcag ggtttcatcg
    17041 tgataagcgc gggcagtaaa cgggtcgagg gccagattaa actggtcttc ccagcgaaat
    17101 tcgaagcggg ctttcgacat ggcgttatcg cgaatttgcg cgcccggatg ccctttcgcc
    17161 aggtcggcgg cgtgagcggc aatcttatag gtgataagcc cctgtttaac atcttcttta
    17221 tttggcaggc ccagatgctc tttcggcgtt acgtagcaga gcattgcaca accaaaccag
    17281 ccaatcatcg ccgcgccaat ccctgacgtg aagtggtcat agcccggcgc aatatcggta
    17341 gttagcggcc ccagagtgta aaacggcgct tcgtggcagt gctctaactc ctcggtcata
    17401 ttgcggcgga tcatctgcat cggcacgtgg cctgggcctt caatcatcac ctgtacgtca
    17461 tattcccagg cgattttggt cagttcaccc agcgtatgca gctcggcaaa ctgcgcttca
    17521 tcgttggcgt cctgaataga accggggcgc agaccgtcgc ccagcgacag cgaaacgtca
    17581 taagcggcac agatttcaca aatttcgcgg aagtgttgat agaggaaatt ttcctgatga
    17641 tgggagaggc accatttcgc cataatcgaa ccgccgcgag agacgatacc ggtcaggcgt
    17701 ttcgcggtca tcggcacata gcgcagcagt acgcccgcat ggatagtgaa gtaatccaca
    17761 ccttgctcgg cctgttccag cagcgtgtcg cggaacgctt cccaggtaag atcttcggcg
    17821 atcccgttaa ccttctccag cgcctggtag atcggcactg taccgatcgg caccgggctg
    17881 ttacgcaaaa tccactcgcg ggtttcgtga atatagcgac cggtggagag atccatcacc
    17941 gtatccgctc cccagcgcgt ggaccatacc agcttttcca cttcttcttc gatggaagag
    18001 gtgaccgccg agttgccgat attggcgtta acttttacca ggaaattgcg accaataatc
    18061 atcggctccg attccggatg attaatgttg gccgggataa tcgcgcgtcc ggcagcaact
    18121 tcatcacgga caaattccgc agtgatattt tccggcagac gtgcgccaaa gctcattccc
    18181 ggatgctggt ggcgtaaaac ttcgctacgt atgcgctcgc ggcccatatt ctcgcggatg
    18241 gcgatgaatt ccatttccgg tgtgatgatg ccctggcggg cgtagtgcag ttgggtgacg
    18301 cggcgtcctg ctttggcgcg ttttggtgtt agtacgccgc taaaacgcag ctcgtcgagg
    18361 ccatcatctg ccagccgcgc ttttgtgtaa tcggaactgc gcacggtaag ttcttcggta
    18421 tcgccgcgcg catcgatcca cggctggcgt agttttgcca gtccttgctg cacgttaatg
    18481 gcgatctgtg gatcgccata cggaccggag gtgtcgtaga ccggaatcgc ttcgttttct
    18541 tcgtactgcg gctgttcttt gctgccgcca attagcgtcg ggctaagctg gatctcacgc
    18601 atcggcacgc gcacgccggg gtgtgtgcca gtgatgtaaa tgcgttttga gttgggaaag
    18661 gcggagcctt ccagggtgtc gataaaatgt tgggcctggg cgcgttgttc gcggcgggtc
    18721 agttttgtta cagacatagc tcattccaaa aagttaagga cgtggcttgt cagacgacgg
    18781 atgaagcaag agacgatcgc cgcaaaggcg atgcgatagc agattaactc ttgttccctt
    18841 cgcaggtatt agcctgatca ggttccgcgg atcccgaata aacggtctca gccagttaag
    18901 gcactccgac aagaaattag ccccacaaac ggggcgttga atgtaaatta cgcgttaaca
    18961 gcgcagaact caagcaggat gtttgacgcg ggcggcgtca agtaccagca gaatcaactt
    19021 atcttccagc acaaagcgtg cttccagcgc ctcgccgatg tcagataaaa cctgttgaaa
    19081 ctcaaggtaa ttatcatgat cgatggcggt ttccaggctg gaatcgtagt aatccataat
    19141 ctgttgggta ttggcttcga gttgcggcca aatcttcgcg gcgcgcgcga gttgcccgtt
    19201 gccttccagc ttatgaagaa tgcgctcata aatactgaaa tgtccggcag acaagtaatc
    19261 gaccaggctc tgacaaaaat catcaagggc tttttcgttt agcctcatgt acgattcttt
    19321 gccaggctta atgccaacca gattgtagta agccacgagc agatgcttac gtacatgtag
    19381 ccagcgatca accagtttgt tacttcctct gacgcgttcc gtcaggttat cgagctggtt
    19441 aagcatgatt gactccgcaa gtttgtattc aaaaactgct ctgtgagaaa tgtaaaaacc
    19501 atgttaaaca tgccagtgat gcaaaggtag tgcaagagct atggatcgta taattgaaaa
    19561 attagatcac ggctggtggg tcgtcagcca tgaacaaaaa ttatggttgc cgaagggaga
    19621 attgccatat ggcgaagcgg caaatttcga tcttgtgggt cagcgcgcac tacaaatcgg
    19681 cgaatggcag ggggaacctg tttggttagt acaactgcag cggcgtcacg atatggggtc
    19741 ggtacgtcag gtcattgatc tcgatgttgg gctgtttcaa ctggccggac gtggcgtgca
    19801 actggcggag ttttaccgat cgcataaata ctgtggttac tgcgggcatg aaatgtatcc
    19861 aagcaaaacc gaatgggcga tgctgtgcag ccattgccgt gagcgttact acccgcaaat
    19921 cgccccctgc attattgttg ccatccgtcg cgatgattcg atcctcctcg cccagcatac
    19981 ccgccatcgt aacggtgtcc atacagtact tgccggattc gtcgaagtgg gtgaaaccct
    20041 cgagcaggca gtcgcgcggg aagtgatgga agagagcgga attaaagtta aaaacttgcg
    20101 ttacgtgacc tcccaaccgt ggccgtttcc tcagtcgtta atgaccgcgt ttatggcgga
    20161 atatgacagc ggcgagatcg tgatcgaccc gaaagaattg ctcgaggcga actggtatcg
    20221 ctatgacgat ttgccgttac tcccgccgcc cggcaccgta gcgcgccgtc tgatagaaga
    20281 tacggtggcg atgtgtcggg cagagtatga gtgatgatac actgaccgcc tgacgcacta
    20341 aggaacagcc aaaatgaccg aacttaaaaa cgatcgttat ctgcgggcgc tgctgcgcca
    20401 gcccgttgat gtcactccag tatggatgat gcgccaggcg ggtcgctatc taccggaata
    20461 taaagccacg cgcgcccagg cgggcgattt tatgtcgctg tgcaaaaatg ccgagctggc
    20521 gtgcgaagtg actttgcaac cgctgcgtcg ctatccgctg gatgcggcga tcctcttttc
    20581 cgatatcctc accgtgccag acgcgatggg tttagggctc tattttgaag ccggagaagg
    20641 tccgcgtttt acctcaccag tcacctgcaa ggccgatgtc gataaactgc caattccgga
    20701 cccggaagat gagctgggtt acgtgatgaa cgcggtgcgt accattcgtc gcgaactgaa
    20761 aggcgaagtg ccgctgattg gtttttccgg cagcccgtgg acgctggcga cttacatggt
    20821 tgaaggcggt agcagcaaag ccttcaccgt gatcaaaaag atgatgtatg ccgatccgca
    20881 ggcgctgcac gcgctactcg ataaactggc gaaaagcgtc actttgtatc tgaatgcgca
    20941 gattaaagcc ggtgctcagg cagtcatgat tttcgacacc tggggcggcg tgcttaccgg
    21001 gcgcgattat caacagttct cgctctatta catgcataaa attgttgatg gtttactgcg
    21061 tgaaaacgac ggtcgccgcg taccggtcac gctgtttacc aaaggtggcg gacagtggct
    21121 ggaagcgatg gcagaaaccg gttgcgatgc gctgggcctc gactggacaa cggacatcgc
    21181 cgatgcgcgc cgtcgcgtgg gcaataaagt cgcgctgcag ggtaatatgg acccgtcgat
    21241 gctgtacgcg ccacctgccc gcattgaaga agaagtcgcg actatacttg caggtttcgg
    21301 tcacggtgaa ggtcatgtct ttaaccttgg tcacggcatt catcaggatg tgctgccaga
    21361 acatgctggc gtgtttgtgg aggcagtgca tcgcttgtct gaacagtatc accgctaagg
    21421 agcgattatg gatctcgcgt cattacgcgc tcaacaaatt gaactggctt cttctgtgat
    21481 ccgcgaggat cgactcgata aagatccgcc ggatctgatc gccggagccg atgtcggatt
    21541 tgagcagggc ggagaagtga cgcgagcggc gatggtgctg ctgaaatacc cctcgcttga
    21601 gctggtggag tataaagtgg cccgtatcgc caccaccatg ccttacattc caggttttct
    21661 ctcgttccgc gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga
    21721 tttagtgttt gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tagccagcca
    21781 ttttggctta ttggtggatg tgccgaccat tggcgtagcg aaaaaacggc tctgcggtaa
    21841 attcgaaccg ctctccagcg aaccgggcgc gctggccccg ctgatggata aaggcgagca
    21901 gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca
    21961 tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct
    22021 gccggagcca acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta
    22081 tacagcaaat cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa
    22141 catgttacaa aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcacgtcac
    22201 ttttatggcc tgcttatgcg aacgtatgta ccccaattac gccgtattct gtcagcaaac
    22261 cggcttcggc gatgggcaga tttaccgccg cattctcgat ctcatttggg aaacgctgac
    22321 cgtcaaagac gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc
    22381 ttcagccgac gatttcgatc tgtacggcgt ttatccggca atcgatgctt gcgtggcgtt
    22441 aagtgaactg gtccattcgc gtttaagtgg cgaaacgctc gaacacgcgg tggaagtgag
    22501 taagacttcc atcacgaccg tggcgatgct ggaaatgact caggctggtc gcgaaatgag
    22561 cgatgaagag ctcaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat
    22621 attccgactt ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc
    22681 agacctgcgt gaggcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa
    22741 aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt
    22801 ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc
    22861 tggcatttcc gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat
    22921 gaacaagact caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc
    22981 taaagctgct ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc
    23041 tgtacaactg gttggtttcg gtaccttcaa agtgaaccac cgcgctgagc gtactggccg
    23101 caacccgcag accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc
    23161 tggcaaggca ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga
    23221 aggggtggtt tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggcgctct
    23281 gtttctcact gcctgtagtc acaactcttc acttcctccc tttaccgcca gtggatttgc
    23341 tgaagaccag ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct
    23401 tgccgtgttt agcccgtggc gcaatggcga taccaccacg cgagagtatc gctggcaggg
    23461 cgataacctt acgctcatca atatcaatgt ttacagcaaa ccgccggtga atattcgcgc
    23521 gcgttttgac gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca
    23581 gcagctttct aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat
    23641 tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga
    23701 acagaccgtg accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat
    23761 agcgcatatc gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga
    23821 agcgcccgaa ggttcgcaat tactgttggt ggcaaactct gatttctgtc gctggcaacc
    23881 caacgagaaa acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag
    23941 ccgccgtaac ccattccggt accgcgcgga atacccgctt cagccatcgc aatatctcgt
    24001 ttcacccgta actcatctaa cgactgacgc aaattctcca tctctttggc gaccgcgtta
    24061 attttgctgc tatccggtgg gttcgtggct aacagggcat tgtattcata acgctttgtc
    24121 accagttgct gttgcagtgc gctgctttga gcgtaaaagt cattatggat tttctgccac
    24181 gctgtctgtt gttcgctggt caaaggcgcg gcattttgct gccacatacc atgtccgccg
    24241 tgagcaaatg cagatgtcga gcccatcgcc attgctgaaa gcgccatcat taccagggca
    24301 attttcgtgt tccgtttcat ggttaatcct ccagtggttg tcttacttcg ggtattgcat
    24361 cttccgtgcc aacggcgaaa cgctgatatg acgggtaatc tggcatgata aacgagtaaa
    24421 aatgactcgc ctgctgcggg tagcgagtca tttttactca ttgaaactta agcctttgtg
    24481 ttacagcgca gggtaagcgc tgataaaaga tggcatgatt tctgctgtca gaaagggatg
    24541 gacaggcaaa gaagaagatg cgttttatgc aacgttctaa agactcctta gctaaatggt
    24601 taagcgcgat cctccccgtg gtcattgttg ggctggtggg gctgtttgcg gtgacagtga
    24661 ttcgcgatta tgggcgcgag actgccgccg ccagacaaac gctgctggaa aaaggcagtg
    24721 tacttattcg tgctcttgaa tccggctcgc gcgttggtat ggggatgcgt atgcatcatg
    24781 cgcagcagca ggccttactt gaagaaatgg ccgggcagcc tggagtacgt tggtttgcgg
    24841 tcacggatga acaaggaaca atcgtgatgc atagcaactc cggcatggtg ggaaaacagc
    24901 tttattcccc gcaggaaatg cagcagttac atccgggaga tgaagaagcg tggcggcgga
    24961 tcgatagcgc agacggcgag cctgttctgg aaatttatcg ccagtttcaa ccgatgtttg
    25021 ctgctggaat gcaccggatg cgccatatgc agcaatatgc cgcgacacca caagcaattt
    25081 tcatcgcttt cgacgccagt aatattgtga gtgccgaaga tcgtgagcag agaaacaccc
    25141 tgattatcct cttcgccctg gcgacggtct tgctggcaag cgtgttgtca ttcttctggt
    25201 atcgccgata tctgcgctcg ggccagctat tgcaggatga aatgaagcgc aaagagaagc
    25261 tggtggcact ggggcatctt gcggcaggcg ttgcccacga aatccgtaac ccactttcct
    25321 cgattaaagg gctggcgaaa tactttgccg aacgcgcgcc agcaggggga gaagcgcatc
    25381 aactggcgca ggtgatggcg aaagaagctg accgtttaaa ccgcgtggta agcgagttgc
    25441 tggaactggt taagccaacg catctggctt tgcaggcggt ggagctcaac acgctgatta
    25501 gccactcatt acagctggta agccaggatg caaacagccg ggagatccag ttacgcttta
    25561 ccgccaacga cacattaccg gaaattcagg ccgatccgga caggctgact caggtcctgt
    25621 tgaatctcta tctcaacgct attcaggcga ttggtcagca tggcgtgatt agcgtgacgg
    25681 ccagcgaaag cggcgcgggc gtgaaaatca gcgttaccga cagcggtaag ggaattgcgg
    25741 cggatcagct tgaagccatc ttcactccgt acttcaccac caaagccgaa ggcaccggac
    25801 tggggctggc ggttgtgcat aatattgttg aacaacacgg tggtacaatt caggtcgcaa
    25861 gccaggaggg aaaaggcgca acgttcaccc tttggcttcc ggtcaatatt acgcgtaagg
    25921 acccacaagg atgacgcacg ataatatcga tattctggtg gtggatgatg acattagcca
    25981 ctgcactatt ttgcaggctt tgctgcgcgg ctggggctat aacgtcgcgc tggcgaacag
    26041 cgggcgacag gcgctggagc aggtgcggga acaggttttt gatcttgtgc tttgcgatgt
    26101 gcgaatggca gagatggatg gtatcgccac gctaaaagag atcaaaacat taaaccccgc
    26161 gattccggtg ctgattatga ccgcgtactc cagcgtcgag acggcggtag aggcgttgaa
    26221 aaccggggcg ctggattatc tcatcaagcc gctggatttc gataacctac aggcgacgct
    26281 ggaaaaggcg ctcgcgcata cacacagtgt tgacgctgaa acgcctgcgg tgtctgccag
    26341 ccagttcggt atggtcggta aaagcccggc gatgcaacac ctgctcagtg aaatcgccct
    26401 cgtcgcgcca tcggaagcca cggtgctgat ccacggcgat tccggcaccg gtaaagagct
    26461 ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa aaaccgctgg taacgctcaa
    26521 ctgcgcggcg ctcaacgaat ccttgctgga gtctgaattg ttcggtcacg aaaaaggggc
    26581 gtttaccggg gccgacaaac gccgggaagg gcgctttgtt gaggcggacg gcggcacgct
    26641 gtttctcgat gaaattggcg atatctcgcc gatgatgcag gttcgtctgc tgcgtgcgat
    26701 tcaggagcgc gaagttcagc gtgtcggcag caaccagacc atctcggttg atgtccggct
    26761 gattgcggcg actcatcgcg atcttgccgc agaggtgaat gccgggcgtt ttcgccagga
    26821 tctctactat cgcctgaacg tggtggcgat tgaagttccg tcgctgcgtc agcggcggga
    26881 agatattcct ctgctgacaa accattttct tcagcgcttt gccgagcgta atcgcaaggc
    26941 ggtaaaaggt tttacgcccc aggcgatgga tctgctgatt cactacgact ggccgggaaa
    27001 tattcgtgaa ctggaaaacg cggtggaacg ggcggtggtg ctgctgaccg gggaatatat
    27061 ttccgaacgc gagctgccgc tggcgattgc gagtacgccg atcccgctgg tacaaagtca
    27121 ggatattcag ccgctggtgg aagtcgaaaa agaggtgatt cttgcggcac tggagaaaac
    27181 gggcggcaac aaaaccgaag ccgcccgtca gttagggatc acgcgcaaaa cgctcttggc
    27241 aaaactgtcg cgttagttct gctcgcgttc gatagcgcgc cagccgatat ctttccggca
    27301 gaagcagtcg tcccaatgga tatcggtcat taaggcataa gcgcgtttct gcgcttctgc
    27361 cacggtatga cccagcgcgg tgacgcacag tacgcgcccg ccgctggtca ctacctgctc
    27421 gtcatccgcc agtttcgtgc ccgcgtggaa cactttgccg tcagacactt cttccagcgg
    27481 caggccgtgg atcacatcgc cggtgcggta atcgcccgga tatccacccg cagccatcac
    27541 cacgccgaga gaagcgcgtt catcccactc ggacgttttc tcgtccagct tgccttcaca
    27601 ggctgccagg cagagttcaa ccagatcgga cttcatgcgc agcataatcg gttgggtttc
    27661 tggatcgcca aagcggcagt taaattcaat aaccttcgga ttgccctgtt tgtcgatcat
    27721 caggcccgcg tagagaaaac cagtgtaggt gttgccttcc gccgccatgc ctttcacggt
    27781 tggccagatg atacgttcca tcgtgcgctg atgaacctca tcggtcacta ctggcgcagg
    27841 ggagtaagcg cccatcccgc ccgtgtttgg tccggtatct ttatcgccta cgcgtttgtg
    27901 atcctggctg gtagccatcg gcagcacatg ctcgccgtcc accatcacga taaagctcgc
    27961 ttcttcgcca tcgaggaact cttcgataac gatgcgatga cccgcgtcgc caaacgcgtt
    28021 gcccgccagc atatcgtgaa cagccgcttc cgcttcttcc agcgtcatcg ccacgataac
    28081 gcctttcccg gcagccagac cgtcagcttt aatgacgatt ggcgcgcctt tctcacgcag
    28141 atacgccagc gcaggttcta cctcggtgaa gttctggtat tccgccgtag ggattttatg
    28201 gcgggccagg aaatctttgg taaacgcttt cgagccttcc agttgcgcgg cacctgcggt
    28261 tgggccgaag attttcagcc cggcggcgcg gaaggtatcg accacgcctt tcaccagcgg
    28321 cgcttccggg ccgacgatgg ttagatctac cttttcgttt tgtgcgaaat ccagcagcgc
    28381 cgggatatcg gttacgccga tagcgacgtt ctgtagggcg ggttccagcg cagtgcctgc
    28441 gttacccgga gcaacaaaaa cagtttcaac cagcggcgac tgggccgctt tccaggccag
    28501 cgcgtgctcg cgcccgccgt taccaatcac taatactttc atctgtcgct ccattaatgg
    28561 cggaagtggc gcatgtcggt gaagagcatc gcaataccgt gctcgtcggc ggcggcaatc
    28621 acttcgtcat cacggataga accgccaggc tggattacac aggtcacacc cgcagcggcg
    28681 gcggcatcaa taccgtcgcg gaacgggaag aacgcgtcag aagccattga cgaacctttc
    28741 acttccaaac cttcatcggc agctttgata ccggcgattt tcgcggagta cacgcggctc
    28801 atctggcctg cgccaatacc gatggtcata ttgtttttgg catagacgat agcgttggat
    28861 ttcacgaact tcgccacttt ccagcagaac agcgcatcac gaagttcctg ttcagtcggc
    28921 tgacgtttgg tgacgacgcg cagttcttcc gcaccgacca tccccagatc gcgatcctga
    28981 accagcagac cgccgttcac gcgtttgaaa tcaagacccg gaatacgttc gccccactga
    29041 ccgcaggtca gaacgcgtac gttctgtttg gcggcggtga ttttcagggc ttcttcgctg
    29101 gcggatggcg caatgatcac ttcaacaaac tgacgagaaa tgatggcctg tgcggtttcc
    29161 gcatccagct cgcggttaaa ggcaatgatg ccgccgaatg cggaggtcgg gtcggttttg
    29221 tacgcgcgat cgtaagcatc aagaatggaa ttgccgatag ccacgccgca agggttggcg
    29281 tgcttcacaa tcacacatgc cggctcggcg aactctttca cgcactccag cgccgcatcg
    29341 gtatcggcga tgttgttata agagagggct ttaccctgaa cctgggttgc ggtggcaacg
    29401 gaggcttctt tcacattctc ttctatatag aaggcagcct gctggtggct gttctcgccg
    29461 taacgcatat cctgcttctt aatgaagttc aggttcagcg tacgcgggaa gcgaccggcg
    29521 gcttctttgc tttcaccgtg ataggccgga accatgctgc cgaagtagtt ggcaatcatg
    29581 ctgtcgtagg cggcggtgtg ttcgaaggct ttgatagcga ggtcgaaacg ggttgcaagc
    29641 gtcagcgaac cttcgttggc atccatctca ttaataatgg cgtcgtaatc gctgcttttc
    29701 accacgattg cgacatcttt atggttcttg gcggcggagc gcaccatcgt tgggccgccg
    29761 atatcaatgt tctcaaccgc atcttccagc gagcagcctt cacgagcaac ggtctgggcg
    29821 aacggataca ggttaacaac caccatatcg ataggctgga tctgatgttc ttccataatg
    29881 gcatcgtcct ggccgcgacg gcccagaatg ccaccatgta ctttcggatg cagggtcttc
    29941 acgcgtccat ccatcatctc cgggaaaccg gtgtaatcgg aaacttcggt taccggcaga
    30001 cctttttctg ctaacagacg ggcagtgccc cctgtagaca gcagctccac accgcgtgcg
    30061 gaaagtgcct gggcgaattc gacgataccg gctttgtcag aaacactgag cagagcgcgg
    30121 cggactggac gacgttgttg catggtaaat cccctggatt tgactattac agagagtgtt
    30181 agctgaattt ttcgcgaaaa actcagctaa cgcccctaac ggggcatcct tatttttcgc
    30241 ccgcattgta acgaaaacgt ttgcgcaacg ctcgcgaatt tttctctttc aatggtgatc
    30301 acaattttga cagtggctac cgtgggaaaa atacagaaat tacattgatg attgtggata
    30361 actctgtgcg taaaaaggta taaagcgggg ttttgctggg gaatgcagca gtcagtcatt
    30421 tttatgcaat ttttctattg cggcctgcgg agaac
//
LOCUS       DADSZD010000038        30341 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.038, whole genome shotgun sequence.
ACCESSION   DADSZD010000038 DADSZD010000000
VERSION     DADSZD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.038"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            31..633
                     /locus_tag="J1Z28_001563"
     CDS             31..633
                     /locus_tag="J1Z28_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBA7716407.1"
                     /translation="MNINYPAEYEIGDIVFTCIGATLFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(635..1156)
                     /gene="ruvC"
                     /locus_tag="J1Z28_001564"
     CDS             complement(635..1156)
                     /gene="ruvC"
                     /locus_tag="J1Z28_001564"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBA7716408.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1191..1931)
                     /locus_tag="J1Z28_001565"
     CDS             complement(1191..1931)
                     /locus_tag="J1Z28_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7716409.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1960..2412)
                     /gene="nudB"
                     /locus_tag="J1Z28_001566"
     CDS             complement(1960..2412)
                     /gene="nudB"
                     /locus_tag="J1Z28_001566"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBA7716410.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2405..4177)
                     /gene="aspS"
                     /locus_tag="J1Z28_001567"
     CDS             complement(2405..4177)
                     /gene="aspS"
                     /locus_tag="J1Z28_001567"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBA7716411.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4487..5053
                     /locus_tag="J1Z28_001568"
     CDS             4487..5053
                     /locus_tag="J1Z28_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA7716412.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5050..5868
                     /locus_tag="J1Z28_001569"
     CDS             5050..5868
                     /locus_tag="J1Z28_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBA7716413.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPYSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5921..6316
                     /locus_tag="J1Z28_001570"
     CDS             5921..6316
                     /locus_tag="J1Z28_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716414.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6357..7100
                     /gene="cmoA"
                     /locus_tag="J1Z28_001571"
     CDS             6357..7100
                     /gene="cmoA"
                     /locus_tag="J1Z28_001571"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBA7716415.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7097..8068
                     /gene="cmoB"
                     /locus_tag="J1Z28_001572"
     CDS             7097..8068
                     /gene="cmoB"
                     /locus_tag="J1Z28_001572"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBA7716416.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVMPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(8233..10662)
                     /locus_tag="J1Z28_001573"
     CDS             complement(8233..10662)
                     /locus_tag="J1Z28_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HBA7716417.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPV
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDVAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADISSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAVQA"
     gene            complement(10687..11787)
                     /locus_tag="J1Z28_001574"
     CDS             complement(10687..11787)
                     /locus_tag="J1Z28_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HBA7716418.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVTPGTD
                     LTVVDASGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNTWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12175..12921)
                     /gene="cutC"
                     /locus_tag="J1Z28_001575"
     CDS             complement(12175..12921)
                     /gene="cutC"
                     /locus_tag="J1Z28_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBA7716419.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLEVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLTELGITRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYLRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(12935..13501)
                     /locus_tag="J1Z28_001576"
     CDS             complement(12935..13501)
                     /locus_tag="J1Z28_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA7716420.1"
                     /translation="MANWQSIDEMQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13717..15450
                     /gene="argS"
                     /locus_tag="J1Z28_001577"
     CDS             13717..15450
                     /gene="argS"
                     /locus_tag="J1Z28_001577"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBA7716421.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15503..15895)
                     /locus_tag="J1Z28_001578"
     CDS             complement(15503..15895)
                     /locus_tag="J1Z28_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBA7716422.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(15895..17973)
                     /gene="flhA"
                     /locus_tag="J1Z28_001579"
     CDS             complement(15895..17973)
                     /gene="flhA"
                     /locus_tag="J1Z28_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HBA7716423.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(17966..19114)
                     /gene="flhB"
                     /locus_tag="J1Z28_001580"
     CDS             complement(17966..19114)
                     /gene="flhB"
                     /locus_tag="J1Z28_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBA7716424.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(19323..19967)
                     /gene="cheZ"
                     /locus_tag="J1Z28_001581"
     CDS             complement(19323..19967)
                     /gene="cheZ"
                     /locus_tag="J1Z28_001581"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBA7716425.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(19978..20367)
                     /gene="cheY"
                     /locus_tag="J1Z28_001582"
     CDS             complement(19978..20367)
                     /gene="cheY"
                     /locus_tag="J1Z28_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheY"
                     /protein_id="HBA7716426.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(20382..21431)
                     /locus_tag="J1Z28_001583"
     CDS             complement(20382..21431)
                     /locus_tag="J1Z28_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HBA7716427.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(21434..22294)
                     /gene="cheR"
                     /locus_tag="J1Z28_001584"
     CDS             complement(21434..22294)
                     /gene="cheR"
                     /locus_tag="J1Z28_001584"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBA7716428.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRALGLADFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVSLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(22313..22539)
                     /locus_tag="J1Z28_001585"
                     /pseudo
     CDS             complement(22313..22539)
                     /locus_tag="J1Z28_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(22585..24246)
                     /gene="tar"
                     /locus_tag="J1Z28_001586"
     CDS             complement(22585..24246)
                     /gene="tar"
                     /locus_tag="J1Z28_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBA7716429.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(24391..24894)
                     /gene="cheW"
                     /locus_tag="J1Z28_001587"
     CDS             complement(24391..24894)
                     /gene="cheW"
                     /locus_tag="J1Z28_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBA7716430.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(24915..26879)
                     /gene="cheA"
                     /locus_tag="J1Z28_001588"
     CDS             complement(24915..26879)
                     /gene="cheA"
                     /locus_tag="J1Z28_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBA7716431.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSMQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(26884..27810)
                     /gene="motB"
                     /locus_tag="J1Z28_001589"
     CDS             complement(26884..27810)
                     /gene="motB"
                     /locus_tag="J1Z28_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBA7716432.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(27807..28694)
                     /gene="motA"
                     /locus_tag="J1Z28_001590"
     CDS             complement(27807..28694)
                     /gene="motA"
                     /locus_tag="J1Z28_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBA7716433.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(28821..29399)
                     /gene="flhC"
                     /locus_tag="J1Z28_001591"
     CDS             complement(28821..29399)
                     /gene="flhC"
                     /locus_tag="J1Z28_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBA7716434.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSNCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(29402..29752)
                     /gene="flhD"
                     /locus_tag="J1Z28_001592"
     CDS             complement(29402..29752)
                     /gene="flhD"
                     /locus_tag="J1Z28_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBA7716435.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7197 a         8236 c         7387 g         7521 t
ORIGIN      
        1 tattcatatc tatttcaatg gaatgacaac gtgaatatta attatcctgc tgaatatgaa
       61 attggtgata tcgtctttac atgtataggt gctaccttat ttggtcaaat atcagctgca
      121 tcaaattgct ggagtaatca cgtcgggatc attatcggtc ataacggtga agactttctg
      181 gttgcagaaa gccgtgttcc cctctcaacc atcactacgc tatcccgttt tattaaacgc
      241 tctgctaatc aacgctatgc tataaagcga ttagacgccg ggctaacaga acaacaaaaa
      301 caacgaattg ttgaacaggt tccctcccgg ctacgcaaac tttaccacac cggttttaaa
      361 tacgaatctt cgcgccagtt ctgttcaaaa tttgtttttg atatttataa agaggcgcta
      421 tgtattccgg tgggtgaaat agagacgttc ggagaattgt taaatagcaa tccaaatgca
      481 aaactcactt tctggaaatt ctggttctta ggttctattc cgtgggagcg taaaaccgtc
      541 acgccagcca gtttgtggca tcatccgggt ttggtgttga ttcacgcggt gggagttgaa
      601 acgcctcagc ctgaactgac cgaggcggta taacttaacg cagtcgccct ctcgccaggt
      661 tcagtcgcga ttcgctcatt tgcatcgcat tctgactaac gtggcagtgg gtgatggcaa
      721 tcgccagcgc atcggcggca tccgcctgtg gattagcggg cagtttcagc aaggtgcgga
      781 ccatatgctg cacctggctt ttttcggcac taccaatacc taccactgtt tgctttacct
      841 gacgtgccgc atattcaaat accggcaatt cctgattcac cgccgccaca atcgccacgc
      901 cgcgcgcctg ccccagtttc agggctgagt cagcgttctt cgccataaag acctgttcaa
      961 tggcgaaata atcaggctgg aattgggtga tgatttccgt cacgcccgca tagatgagct
     1021 tcagacgaga cggtaaatca tccactttgg tgcgtatgca tccgctaccc aggtaggaca
     1081 gttgcctgcc tacctggcgg atgacgccat agccggtcac gcgcgaaccc gggtcaatgc
     1141 cgagaataat agccatcacg cgtctccgtt ttgctgttta gcaggcctca tcagagagtc
     1201 gctgcaacct catcagagat ttcaccgtta tggtaaactt cctgcacgtc gtcgcaatct
     1261 tccagcatat cgatcagacg catcagtttc ggtgcggttt ctgcatccat atcagctttg
     1321 gtggacggga tcatggaaac ttccgcgctg tctgctttca gacctgccgc ttccagagcg
     1381 tcgcgtactt tgcccatttc ttcccatgca gtgtagacat caatcgcgcc gtcatcatag
     1441 gtcacaacgt cttcagcacc ggcttccagg gctgcttcca tgatggtgtc ttcatcgcct
     1501 ttctcgaagg agatcacgcc ttttttgctg aacaaataag ctacggaacc atcagtaccg
     1561 aggttaccgc cacatttgct gaatgcatga cgcacttcag caacggtacg gttgcggttg
     1621 tcagacagac attcaatcat gattgccgtg ccgccaggac cgtaaccttc gtagatgatg
     1681 gtttccatgt ttgcatcatc atcaccgccc acacctcgtg caattgcgcg gttcagagtg
     1741 tcacgggtca tgttgttaga cagtgcttta tcaattgctg cacgcaaacg cgggttagcg
     1801 tccggatcac caccgcccag cttagccgcg gttaccagct cacgaatgat tttagtgaag
     1861 attttaccgc gcttagcatc ctgcgcagct ttacgatgtc tggtgttggc ccatttacta
     1921 tgacctgcca taaaaatatc tccagatagc cctgcctgtt caggcagcgt taattacaaa
     1981 ctgttcaatc gcctgccggt tgctccagga cttagtgagc gccgccgcag cagacgcatc
     2041 aagccacttg taagccagat gttcagtgaa aacgatctgg cgctcgtgcg gaagcgcaag
     2101 acagaaccat gattccgtat tacgcgtcac gcccggcgca tagcgatgac gtaaatgtga
     2161 aaaaatttca aactctaccg tgcgctgaca gtcaattaag gtcagttgtt cagcgacaac
     2221 atcaatggtg acctcttcct ttacttcgcg catggcagct tgcggcgcgg tttccccctc
     2281 ttccacgctg ccggttaccg actgccagaa atcgggatcg tcacgccgct gcaacatcag
     2341 cacccgtttc gtatcttgtg cgtagatgac cactaagatc gaaacgggac gcttataagc
     2401 catatcagtt attctcagcc ttcttcacaa cctgaatgct cagctcagcc agtgcagtcg
     2461 ggttagcaaa gctcggtgct tcagtcatca gacacgctgc cgccgtggtt ttcgggaagg
     2521 cgataacgtc acgtatattg tcggtgccgg tcagcagcat ggtcagacgg tcaagaccga
     2581 atgccagacc tgcgtgcggc ggagtaccgt atttcagggc gtcgagcagg aagccgaatt
     2641 tctcgcgctg ttcctcttcg ttgataccca gaataccaaa caccgtctgc tgcatatcac
     2701 cattatggat acgcacagaa ccaccgccca cttcgtaacc attgatgacc atatcgtaag
     2761 cgttcgccac cgcattttcc ggtgcagctt tcagttctgc tgccgtcatg tctttcggtg
     2821 aggtgaacgg atggtgcatt gccgtcaggc cgccttcacc gtcgtcttca aacatcggga
     2881 agtcgataac ccacagcggt gcccatttgc tttcgtcggt cagaccaagg tctttaccca
     2941 ctttcaggcg cagtgcgccc atcgcgtcgg caacaatttt cttgttatcg gcaccgaaga
     3001 aaatcatatc gccatcttgc gcgccagtac gttccaggat ggcttcgatg atttctgcat
     3061 taaggaactt cgctaccggg ctgttgatac cttccagacc tttcgcgcgt tcgttaactt
     3121 tgatgtaagc cagacctttc gcgccgtaga ttttaacgaa gttaccgtat tcgtcgatct
     3181 gcttacgggt caacgatgcg ccgcccggaa cgcgcagagc ggcaacacgg cctttcggat
     3241 cattcgccgg acctgcaaat actgcaaact caacagattt cagcagatcg gcaacgtcgg
     3301 tcagttccat cgggttacgc agatccggtt tatcagaacc ataacggcgt tctgcttctg
     3361 caaaggtcat taccgggaaa tcgcccagat ccacgccctt cacttccagc cacagatgac
     3421 gcaccagcgc ttccatcact tcacgcactt gcggcgcggt catgaaagaa gtttccacat
     3481 cgatctgagt aaattcaggc tgacggtcag cacgcaggtc ttcgtcacgg aagcatttaa
     3541 cgatctgata gtagcggtca aagccggaca tcatcagtag ctgtttgaac aactgcgggg
     3601 attgcggcag cgcgtagaat ttacctttgt gcacacgaga aggcaccagg tagtcacgcg
     3661 cgccttcagg cgtggctttg gtcagcatcg gagtttcgat gtcgaggaag ccgtggtcat
     3721 ccataaaacg gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgc tgagccattt
     3781 ccgggcgacg caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg ttgacgtggt
     3841 tagagtcaag cggcagaaca tctgcacggt tgatgatagt cagcgaggac gccagtactt
     3901 cgatttcgcc agtcgccata tcgcggttaa tatttttttc gtcacgcgca cgtacggtgc
     3961 ccgtgacctg aatgcagaac tcattacgca gttcagaggc cagctttaac gcgtccgcac
     4021 gatccggatc gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg atgaagatca
     4081 ggctaccaag atcacgacga cggttgaccc aaccacacag agtcacctgc tgccccacgt
     4141 gggacaaacg gagctgtcca caatattctg tacgcatgag atatccctta acttagctgc
     4201 cggcggatgc cccctgctgc gcaggtgacc aagtcgcagc gttagctgta tgtcacaact
     4261 gaatgaaaaa aggcggctat tatactggaa attctgccgc accgtaagag cctggcccgc
     4321 gctggaacgc ctcgttacca ctttatatcg ggcctgaaat cagactctac gccagtttgc
     4381 tataaaggtg ttgcccgaac tcataaaaat taacaaaatt tgtcgttccg ccatcggcta
     4441 atcgcattaa ggtgagaggc acgattttgt tttgtcagga gtcatcatgc ttgaacttaa
     4501 tgctaaaacc accgcgctgg tggtgattga tttacaagaa ggcatcttac cttttgccgg
     4561 aggtccacat actgccgatg aggtggttaa tcgcgccggg aagctggcgg cgaaatttcg
     4621 cgccagcggt cagcccgtgt ttctggtgcg cgttggctgg tctgccgatt acgccgaagc
     4681 attaaaacag ccggttgatg ccccctcccc cgcaaaagtg ttgcccgaaa actggtggca
     4741 acatcctgct gcattaggtg caaccgacag cgatatcgaa atcatcaaac gtcaatgggg
     4801 tgcgttttac ggtacggatc tggagttgca attacgccgc cggggtatcg atacaatagt
     4861 gttatgtggg atctcgacca atatcggtgt tgaatccacc gcccgcaatg cctgggaact
     4921 cggttttaat ctggtgattg ccgaagatgc ctgtagcgcc gctagcgccg agcagcacaa
     4981 taacagcatt aatcatatct acccgcgcat cgcccgtgtg cgtagcgttg aagagatcct
     5041 ccacgcgtta tgatttacat cggtttgcca caatggtcgc atcctaaatg ggtgcggttg
     5101 gggatcacca gccttgaaga gtatgcccgc cactttaact gtgtggaggg taacaccacg
     5161 ctatatgccc tgccgaaacc cgaggttgtc ctgcgctggc gtgagcagac cacagatgac
     5221 ttccgcttct gttttaagtt tccggcgacc atttcgcatc aggcagcatt acggcattgc
     5281 gatgatttag tgactgaatt tttgacccgc atgtcaccgt tggctccgcg cattggacaa
     5341 tactggctgc aactgcctgc cacattcggc ccacgggagc tgcctgcgct ttggcatttt
     5401 ctcgattctc ttcccggtga atttaattat ggggtggaag tccgccatcc acagtttttc
     5461 gccaaagggg aagaggaaca aacgcttaat cgcggtttac atcagcgcgg cgttaatcgg
     5521 gtgattttag acagccgccc ggttcatgca gcacgtccat acagtgaagc tattcgcgac
     5581 gctcaacgaa aaaaacctaa agttccggta catgctgtac tgacggcgaa aaatccactg
     5641 atccgtttta tcggtagtga tgatatgacg caaaaccggg aattatttca ggtctggtta
     5701 caaaaattag cgcagtggca tcagaccact acgccttatc tttttttaca tacgccagat
     5761 attgcccagg ccccggaact ggtacatacc ctgtgggaag acttacgtaa aacgcttcca
     5821 gagatcggag cagttccggc tattccacag caatcttctc ttttctgaat ttgccaccta
     5881 tcatagacag gtgccatcgg ccattttaaa gggagtttgt atggtaagcg cgctgtatgc
     5941 cgttttaagt gcgttgttat taatgaagtt ctcttttgat gtcgttcgcc tgcgaatgca
     6001 gtaccgcgtt gcctatggcg acggcggttt tagcgaactg caaagcgcta ttcgcattca
     6061 tggtaacgcg gtggaatata ttcctatcgc gattgtgttg atgctgttta tggaaatgaa
     6121 tggcgcagaa acctggatgg tgcatatttg cggcatcgtt ttgcttgctg gtcgtctgat
     6181 gcattattac ggttttcatc accgtctgtt ccgctggcga cgttctggca tgagcgccac
     6241 ttggtgtgcg ctgttgctga tggtgctggc gaatctttgg tatatgccct gggagttggt
     6301 tttctccctg cgttagcgca caatacgcca ctttcttttt cccggatttt tacgttatgt
     6361 ctcaccgcga cacgctattt tctgccccta tcgccagact gggcgactgg acctttgatg
     6421 aacgggtagc tgaagtcttc ccggatatga tccagcgttc cgttcccggc tattccaata
     6481 ttatttccat gattggtatg ttagccgagc gcttcgttca acctggtacg caggtttacg
     6541 atctgggttg ttctctgggc gcggcgacgc tctcggtgcg tcgcaacatt catcatgata
     6601 attgcaaaat tattgccatc gacaactccc cggcgatgat tgaacgctgc cgtcgtcata
     6661 ttgacgccta taaagcccct acgccagtag acgttattga aggtgatatt cgcgatatcg
     6721 ccattgaaaa cgcatcgatg gtggtgctga attttaccct gcaattcctg gaaccttccg
     6781 agcgccaggc gttactggat aaaatttatc aagggctgaa cccgggcggt gcgctggtgc
     6841 tttcggaaaa attcagtttc gaagatgcca aagttggtga actgctgttc aacatgcacc
     6901 acgactttaa acgtgccaac ggttacagcg aactggagat cagccagaaa cgcagcatgc
     6961 tggaaaacgt gatgctgacc gattccgtgg aaacccataa agcacgcctg cataaagccg
     7021 gttttgagca tagcgagctg tggttccagt gctttaactt tggttcactg gtggcattaa
     7081 aagcagagga cgctgcatga tcgactttgg taacttttat tctctgattg ccaaaaatca
     7141 tctttcacac tggctcgaaa cgctgcccgc gcagattgct aactggcagc gcgagcagca
     7201 gcacgggctg tttaagcagt ggtccaacgc ggtggaattt ctgcctgaaa ttaaaccgta
     7261 tcgtctggat ttattgcata gcgtaaccgc cgaaagcgaa gagccactga gcgccgggca
     7321 aattaagcgc attgaaacgc tgatgcgcaa cctgatgccg tggcgcaaag ggccgttctc
     7381 actgtatggc gtcaacatcg ataccgaatg gcgttccgac tggaaatggg atcgcgttat
     7441 gccccatctt tctgatttaa ccgggcgcac cattcttgat gtcggctgtg gcagcggtta
     7501 tcacatgtgg cgcatgattg gcgcaggggc gcatctggcg gtgggtatcg atcccacgca
     7561 gctattcctc tgccagtttg aagcagtgcg taaactgctg ggtaacgatc agcgcgcaca
     7621 tttgttaccg ttaggtattg aacaacttcc ggcactgaaa gcctttgata ccgtcttttc
     7681 gatgggcgtg ctttatcatc gtcgttcacc gctggagcat ctctggcagt taaaagacca
     7741 actggtgaat gaaggcgaac tggtgctgga aacgctggtt attgatggcg acgaaaacac
     7801 ggtgctggtg ccgggcgatc gttacgctca aatgcgtaat gtctatttca ttccttccgc
     7861 gctggcgctg aaaaactggc tgaagaagtg tggttttgtt gatattcgca ttgcagatgt
     7921 gagcgttacc accacagaag agcagcgacg caccgaatgg atggtcaccg agtctctggc
     7981 cgattttctc gacccgcatg atccgggtaa aacggtggaa ggttatcctg cgcctaaacg
     8041 cgcggtgctg attgcgcgca agccgtaaag gtctggtaat actgccggat gcggcgtgaa
     8101 cgccttatcc ggcctacaaa gtcttgctaa ttcaatatat tgcaggggct atgtaggcct
     8161 gataagcata gcgcatcagg cagttttgcg tttgcataac ctcagcgccc gtttccgggc
     8221 gcttttcacg tcttatgcct gaacagctgg ctgatcaaac gccgttaact tcagcgcatt
     8281 gcccgtatat ttttcgatat acaccagcgc agagttaccg gcacaggcat tcgccagctg
     8341 cgagctggag atatccgccg ttaacacgtt cgcactaccg tttttacaca agccattttc
     8401 cagatctggc catgcacctt catgtacgca taccacgcct tttttgatcc cgtcagtcac
     8461 caccgcgcct gtcagaatct gaccgcgctt gttccagaca cgcaccagat cgccatttgc
     8521 aataccaaag cgagcagcat cttcggtgtg aatagtgatt ggttcacgat cggcaaccgc
     8581 atatttttta cgcagttccg catagttaag ctgactgtgt aaacggtgtg ccggatgtgc
     8641 ggtcagaagc tgcaactgct tctcgtcggc ggtacccttc cactcatcag gcgcaagcca
     8701 ggttgggtgt gccgggcaat ccttatagcc aaatttttcc agcgttctgg agtaaatctc
     8761 aattttgccg cttggtgtac ccagcgcatt tttcaccgga tcggcgcgga aatcaccata
     8821 acgaatgtac tgttcgttct tctcgctgcg gcgcatttcg atcagtttat tttgctgcca
     8881 gaaggcatta aacatcggca tagtcacgcg ttgcgcacgg gcacctttct gagcgacatc
     8941 atagaaaaat ttcagccacg ccatttcatc tttaccttcg gtatagatct cttttccgcc
     9001 aggtttgagt aattcagcaa gatcggcaaa cacgtcaaaa tcgttgcgcg cttcaaattg
     9061 cggagcgaca gcctgcttca tcggcacaat atgctggttg ctgtaatcac cggtcatcgt
     9121 caggtcattg cgctcaaacg atgtggtgat cggtaatacg atatctgcgt gtttggctgc
     9181 tgcggtccag tagcattcag aaacgacgat catctccggt ttctgccatg ctttaatcag
     9241 acgattagta tcctggtgat gggtaaagtt accgccaccc gcccaccaga tcattttgat
     9301 attcggatac gtttgttctt taccgttatg ctgatatttg ccgccaggat tttccagcgc
     9361 atcgacgata cgggcaacag gaatcgccgt cattccgcca tcatcagcgg cctcgctggc
     9421 ctgtccggca atcgccgcag acatttctgg tagcacgccg ccaacacgcg tcgggttgcc
     9481 accgttggag taatgataag agaaaccaaa cccgccgccc ggtgtaccaa tctgccctaa
     9541 catcgccgcc agcgtcacca gcatccagtg tttctgttca ccatattgct ggcgctgaat
     9601 tccccagcct gccatcagca ttgtgcgatt tgccgccatc agttcagcga gtttgactat
     9661 ctgcgcttcc ggtacgccag taatttccgc cgcccaggcc gcgctcttcg gcgtattatc
     9721 gctcttacct gtcagatact cttcaaactg cggatacccg gtagtgtatt tttcaaggaa
     9781 cactttatcg tgtttgcctt gtgtcatcag ggtatgcgca atccctaaca tcagtgccac
     9841 gtcggtaccc atattcggtg cgatccaggt ggcattatcg ccaaagaatt cgatggtttc
     9901 ggagcagata ggatcaatgg caatcactgg tttgccagat tttttcagct gatggaagta
     9961 ttccagccct tgctcatcgg tactgctcca ggcaattttg agcgtgttca gcgggttcat
    10021 cccccacagc accaccacct ggctgttttc cagaatcagc ggccaggagg tctgctgttc
    10081 atacacctct acagaaccga ccacatgcgg catgatcacc tgtgccgctc cggttgaata
    10141 atcgccgcta tgcccggaat aaccgcccgc caggttcata taacgttgca gtaaggtttg
    10201 cgctttatgc aacacgccag aagaacgcca gccataagat ccggcaaaaa tggccgatgg
    10261 tccgttagct ttgcgaatgc gatcgtgttg ttcatgaatc agttttaatg cttgttccca
    10321 gctcacctgt acataggtat cttcgccacg acctttcacc ggttgcagtg gattatcgag
    10381 atagcttttt ctcaccatcg gatgctgaat acgcgccgtg gtgtgtacct gatccgccgc
    10441 cgtagactgt aaggagttcg gtatggtttt cgccagcgcg cctgtcgaag aaacaatctt
    10501 gccgtccttc acttctacgt tcatcgctcc ccaacggccc gcggtgagaa ttttgccgcc
    10561 cttttcttct gcccatgcgg gtagcggtgc agccgatgtc accaccagcg tccctgccgc
    10621 aataccgcta tgtttaataa attcacgtct tgttaatgtc ataacttcct ccctgatcaa
    10681 cgaggatcac tgtttctcgg taatatcttt ggcgttgtac tggaagtacc gcgtcaaaat
    10741 gtccagttcg ttttcgctca tgctggttcg cgcacccatt cctttggcaa tggacggcca
    10801 tgtattgacg gtgtaatggt cggcggcaat aggggcatgg cagccagcgc aataggtatc
    10861 gtcaagtttt tcagcgtatt gccatagcgg tttacggtcc gctaatgcgg gatcgataag
    10921 cgccccctgc aaagacgcct gacgccattg attgccgtat tcgtcagcct gccattcccc
    10981 gtttaccgtt agcgccttga taccttcttc acttaatgtg gcgagcgcca gccgttgacc
    11041 tgccgccagg tagagcgtgt tttcactgcc ctgcatttga taaccctgca accgaacaat
    11101 tggctgtttg ccactggcat caacgacggt aagatcggta ccaggagtca cggtagccag
    11161 ttcgcctatg cgagaagttt tgaaaggata aatatgtgcg ccattagtaa ctgaagtagc
    11221 ggcctgactt tccagctcat gcgccgcgtt gtcatccatt tttatttctg gcggaaaatg
    11281 ggcaatgcct ttatggcaat cgatacaggt ttcgccgtcc ttttgcgctt tattatgcat
    11341 tttttgcgca gattcacttt gcgaggcaat atccatggca tcaaaagaat ggcaactacg
    11401 gcaggttgca gagtcagttg cttttaattc tttccatact gtttcggcca tttcctggcg
    11461 atgagcttcg aacttatcat cactgtctat tttgccgcta acaaattcat gataaatatc
    11521 tttagatgct tttaatttag caaataaata atccatccct gactttggaa tatggcaatc
    11581 ggcacattcc gcacgtatcc ctttctggtt tgaaaagtgg acagttccct gatattcctc
    11641 aaaaggttta ctcatcgagt ggcaggaaag gcaaaatgcg gtatccgacg ttttatgtaa
    11701 ggctttttgc gccagcaata acccgccgcc accaaccaca accgctatca gcaaaaacaa
    11761 taacccaatg cgttttttcc ctcgcataga ttcctctaat taaattccag gacaccgata
    11821 ccattgtata aataaataca caacgaattg actacttgct taattcggca caacactgcc
    11881 tgaaataatc gataaagaat atgatttatt acaatgtaat cattaattgc taaggaataa
    11941 cccagttgtt atttagaata tatatttgtc agttttggca atttaaatca cataatgaat
    12001 gtgaatatat aaaatatttt tagcacaacg ctatgcgcac tgtcacccgc tcaacagatc
    12061 acggcaatcg ttcgtttttt atactgctca gggataaaaa aaggcccctg ttgaaattgc
    12121 aggggcctgg tacgagcaag catcatattg ggcgacatga tgcaacggta aaaatcattt
    12181 ggcctgatgg cgttcaatga ttcctttcat ttcagcaacc gccgccccgt ctacgacata
    12241 acgcaaatac tcgtccgcgt gtgcatctga tgacatggac aatccttgat tacgataacg
    12301 catcggtgag gcttgccacg ctcccgcgga gctatggact tccagcactc cggcatcgag
    12361 gaagtggtgc aggttttccg cacggactcc tgctccggcc ataatgattg gagcatcacg
    12421 atgggcaata agttccataa tttttgataa accttgcagt gcgtctgatt tttgccctga
    12481 tgtcagtacc cgggtaatgc ccaattccgt aagattattg agtgtattta aagggttagc
    12541 gcacatatcg aaggcgcgat gaaaagtcac tgccagcgga ccggcagcag ccattatttt
    12601 ttccattcgt ggcatatcga cattcccgtc aacctcgaga acgcccgtca ccagtccagg
    12661 aaaacctaat tcgcggactg tgcgcacatc ttcaagaatg gcggcaaact caccgtcgct
    12721 gtagcaaaaa tcaccaccgc gtgggcggat aatcggatgc actgggatcg tcacccgctg
    12781 gcgcacggat ttcagtaccc ccaacgacgg cgttaagccc ccctcttttg gagctgcgca
    12841 taattcaact ctgtcagcgc cgttttgctg cgccgttagc gcgcattcca tgctgtaaca
    12901 gcaaatttcc agtaatgcca tttttgctcc ttaattacgc cgactgctcg ctggcaacga
    12961 tctcttcaat ggaccacgga tgaaatttaa tggtcgtttt gccatcggtg accgccagcg
    13021 tcggattcgg taatcgctca tgctcacctt ttggcgaact ggttttaact gagatccccg
    13081 gcaggctgag tccttcatca gaaagcaatg ccagcgcacg ggcattcagc gtttctggat
    13141 cacccggcag aacgatttca atatgttccc aaccttcgtg cgggtaacgt ttttccccgg
    13201 gccacggtag ctccacaatg gaaaactgcc agtgcgcaac ctgtaccggt tcatgcaatt
    13261 taaacagaca aatcggcctg ccattgatca tattttctga caaaagttcg ccgcactgtt
    13321 caaacccgcg acgccagcgt tcagcggtga cgttttgatg gcaacgcaaa gaaatgtgat
    13381 cggcagtcag cggagtgata tccaacccca gacggcggga aagttcatct aatgcgtggg
    13441 taaatcgcgg taaatccgat gcaatatcct gcatctcgtc gatagattgc cagttcgcca
    13501 taatcactct tcgtctttca gtaaaagcgt taatttaccc tgttgccctg cgccaaccaa
    13561 ccgctgattt cacgccgctt ctgatgcaat agtgaaaacg gcaatacgcc acgcgcacgt
    13621 tgctgacgaa aacagccatt tgcagtatac tcccgcccta atttctttaa ctggtgcggg
    13681 caatttttgc tcgcttcatc aatgtaaggt attccggtga atattcaggc tcttctctca
    13741 gaaaaagtcc gtcaggccat gattgcggca ggcgcgcctg cggattgcga accgcaggtt
    13801 cgtcagtcag caaaagttca gttcggcgac tatcaggcta acggcatgat ggcagttgct
    13861 aaaaaactgg gtatggcacc gcgacaatta gcagagcagg tgctgactca tctggatctt
    13921 aacggtatcg ccagcaaagt tgagatcgcc ggtccaggct ttatcaacat tttccttgat
    13981 ccggcattcc tggctgaaca tgttcagcag gcgctggcgt ccgatcgtct cggtgttgct
    14041 acgccagaaa aacagaccat tgtggttgac tactctgcgc caaacgtggc gaaagagatg
    14101 catgtcggtc acctgcgctc taccattatt ggtgacgcag cagtgcgtac tctggagttc
    14161 ctcggtcaca aagtgattcg cgcaaaccac gtcggcgact ggggcactca gttcggtatg
    14221 ctgattgcat ggctggaaaa gcagcagcag gaaaacgccg gtgaaatgga gctggctgac
    14281 cttgaaggtt tctaccgcga tgcgaaaaag cattacgacg aggatgaaga gtttgccgag
    14341 cgtgctcgta actacgtggt aaaactgcaa agcggtgacg aatatttccg cgagatgtgg
    14401 cgtaaactgg tcgacatcac catgacgcag aaccagatca cctacgatcg tctgaacgtg
    14461 acgttgaccc gcgatgacgt catgggtgaa agtctctaca acccaatgct gccgggtatc
    14521 gtggcggacc tgaaagccaa aggtctggca gtagaaagtg aaggcgcgac cgttgtattc
    14581 cttgatgagt tcaaaaacaa ggaaggcgaa ccgatgggcg ttatcattca gaagaaagat
    14641 ggcggctatc tctacaccac cactgatatc gcctgcgcga aatatcgtta tgaaacactg
    14701 catgccgatc gcgtgctgta ttacatcgat tcccgtcagc atcaacacct gatgcaggca
    14761 tgggcgatcg tccgtaaagc gggctatgta ccggaatccg taccgctgga acaccacatg
    14821 ttcggcatga tgctgggcaa agatggtaaa ccgttcaaaa cccgcgctgg cggtacggtg
    14881 aaactggccg atctgctgga tgaagccctg gagcgtgcac gtcgtctggt ggcagaaaag
    14941 aacccggata tgccagccga cgagctggaa aaactggcta acgcggttgg tattggtgcg
    15001 gtgaaatatg cggatctctc caaaaaccgc accacggact acatcttcga ctgggacaac
    15061 atgctggcgt ttgagggtaa taccgcgcca tacatgcagt atgcttacac gcgtgtattg
    15121 tccgtgttcc gtaaagcaga aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc
    15181 cgtgaagatc gtgaagcgca actggcagct cgcctgctgc agtttgaaga aaccctcact
    15241 gtggttgccc gtgaaggcac gccgcatgtg atgtgtgctt acctgtacga tctagccggt
    15301 ctgttctctg gcttctacga gcactgcccg atcctcagcg cagaaaacga agaagtgcgt
    15361 aacagccgtc tgaaactggc acaactgacg gcgaagacgc tgaagctggg tctggatacg
    15421 ctgggtattg agacagtaga gcgtatgtaa tcgcgttata cggcaacagc cgatgtcatt
    15481 gcagacatcg gcttttttat cattaacgat aatttacaat cacttcattc cgttgaatct
    15541 ttagcggcgg aattagccgc ccaccgccag gcacttccca gataaagcgc aacggttctg
    15601 ccgccggtat accggaaaag gccacggtgg ttccactctg cccctctaat tcgacacaac
    15661 gagattgtga acacaagcga acccgcagtc ctgcaggtgt cggaccgata agctgataac
    15721 gccacgccac cagcgtcatc aatcctgaag caggttgtcg cgtagaaaga ggcgctgacg
    15781 acatcgattc accgcgatga tttagcgtaa cgcccacgct actcgcctgc cacatcccct
    15841 ccccggcggc ttgcaccagc agcggaaaca ataatattgc taataaggct ctcattattt
    15901 gccgccaatc gtcgccgtca tacggatatg tcggttatct gatagttcca gattcgacag
    15961 caccactaac tgcggcaagc tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg
    16021 gttcaccaac agtaccggcg gcgcacccaa catctcctga cgggatagcg cttcctgagt
    16081 ttgcgccagt aaacgatccg ccagccctgg ctccagtccc cccccgccct gcaatgcctg
    16141 tagcaacaaa cgttccagcg gtgtatcgag gccaataaca tggacttcat ctttgccagg
    16201 aaaccactgc tgggtaatcg cccgtcccaa cgctacgcgc acgacggcgg ttaattcatg
    16261 cggatcgctt tggatgggcg catgttccgc cagcgtttcg agaatggtgc gcatatcgcg
    16321 aatcggcact ttttcatcga gcagattttg cagcacttta tgcagtgtgg taagcgtgac
    16381 gacgccagga acgagatctt ccgtcagctt tggcatctcc tgggcgacgc gatccagcag
    16441 ctgttgcgcc tcctgacgac caaacagctc tgcggcatgc tggctaatga ggtggttaag
    16501 atgcgttgcc accaccgtgc tggcctcaac cacggtgtac ccctgaatct gcgcctgctc
    16561 tttaagcgca ctttcaatcc agatagcatt caggccaaat gccggatcga cggtcgcctc
    16621 accaggtaac gtcccggcag cggttccagg gttaatcgcc aaccaacgcc ccggataagc
    16681 atcaccactg cctatctcca cgcctttcat caaaatgcga tagcgggcag gttgcagatc
    16741 catattgtcg cgaatgtgta ccactggcgg cagaaacccc atctcctggg caaatttctt
    16801 gcggatactg cgtatacggc ccaacaactc accatcctgc tggaaatcga ccatcgggat
    16861 cagtcgataa cccacttcca ttcccagaga atcttccagt tgtacatcgt tccacgtcgc
    16921 ttcgacaacg gtattattct ctgccatttt taccggtttt ggttcagcag gcgctttttg
    16981 ttcacgtccg cggatccacc aggccagccc cagcaatccg gcagtgaaca gcaaaaatac
    17041 caggttcggc attccaggca ccaggccgag taaaccgagc acggcggcgc ttaataacat
    17101 aacgctcgga ttactgaaaa gctgattcac catctgctcg ccaacatcct gatcggtgct
    17161 gacacgtgta acgatgacgc ccgcggcggt agaaatcacc agtgccggga tttgtgccac
    17221 cagaccgtcg ccaatggtca atagcgtata actttccgcc gcgtgcccca tgctcatgcc
    17281 atgttgcagc acaccgacca gcaatccgcc gacaacgtta atgaccatga tgaggatccc
    17341 ggcgatggca tcgccgcgaa caaacttact tgccccgtcc atcgagccgt aaaaatcggc
    17401 ttcctgagtc acttcggagc ggcgtttttt cgcttcatct tcaccaatca atccggcgtt
    17461 aaggtcggcg tcaatcgcca tctgcttacc cggcatacca tcgagaacaa agcgcgcgcc
    17521 cacttccgca atacgcccgg cacctttggt gataaccata aagttgatga tcacgagaat
    17581 gacgaacacc acgataccga tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc
    17641 gaccaccttt ccagccgccg ccgcgccggt atgcccttcc atcaaaatga tacgggttga
    17701 agccacgtta agtgccagac gcaacagcgt ggtaaacaac aggatggtcg gaaacgcagc
    17761 aaactcgagc gtgcgctggg taaacatcgc caccagcaac accatgatcg acaaagcaat
    17821 attgaaggta aacagcaggt cgagtatgaa tgcgggcagt ggcagtacca tcatcgacaa
    17881 gatcaacagg atcaaaatcg gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc
    17941 aggcaggcgc agcatcgcgg ccagattact catgggtcgg tttctcgtta ataaaatcca
    18001 gggcttccgg caccggaaga tgagtaggtt gtacagggcg ttgtccacca gccagacgcc
    18061 agcgtttcag ttgccagacc caggccagca cttccgccac cgcggcgtac agttgacccg
    18121 ggatttgttg accgatctcc gcatgtcgat acagcgcacg cgccagcggc ggcgcttcaa
    18181 gcgtcgggac gttattttca gcgccaattt cacgaatgcg caacgcgacc agccctgcac
    18241 ctttagcgac cactttcggt gcgctcattt tgttttcgtc atactgcaac gctaccgaat
    18301 agtgggtcgg gttattgaca atgacatccg ctttcggcac atcggccatc atccgacgcc
    18361 gtgcggccgc tcgctgcatc tgacggatcc gccctttaac atgggggtcg ccttcgcttt
    18421 gtttgaactc atcacgaata tcctgccgtg acattcgcag ctttttcagg tggctgaaga
    18481 tttggaaaaa gacgtcaaat cccaccattg gaatgacacc aagcaccacc agcagcgcgc
    18541 atagccctac cagatccatc gcattaccca tggcggtaat cggagactcg gccattaagc
    18601 gcatcatctg cggccagtga tgccagagaa aaaaccccgt cacgctgcca accaggatgg
    18661 ttttcaaaat tgccttaagc aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc
    18721 ccggtagcgg gttgagtttg gaaaacttcg gctgcaagga ttttccgcta aataccagcc
    18781 cccccagcat aaccggagaa ataatcgcca ccagcaccac gccgctaatc agcggtagca
    18841 gcgccagcat ggcttctctg atcagcagaa taatctgccc gaggatcaga ttcgggtcat
    18901 tgataatact gtgatcaaaa tgcagccctg cggagagcat gcccgacaat cgacgggcca
    18961 gcgacacacc gccaaaccag ataacactaa cgcccaccag caaaatcagc agtgaagtca
    19021 gttcacggga acgcgggatt tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg
    19081 gggcttctgt tttgtcgtcg ctctcgtcag acacgtcgcc aatcctggaa agagtattaa
    19141 gcgtaaatga tgccagagcg caaagcgttc aatggtttga gtaaggggca aaacaggcgg
    19201 gatttagggc ttttgccaca gcacatcagg catggtgaca tgtttgtcgg gtacggcgta
    19261 aacgccttat ccaaccttga agatctgatg tgacgcggcc acgtcacatc aggcaataca
    19321 aatcaaaatc caagactatc caacaaatcg tccacctgat cctgactggc taccacaccg
    19381 gctttgctgg tatcgacctg aggtccatta agcaaactct ggttttcacg ttttggacgc
    19441 gactcctgtt ccgggatgtt ttccaacagc accatcagca actgacgttc gatctcctga
    19501 atgacatcca tcatccgctt aatgacctgt ccggtgagat cctgaaaatc ctgcgccatc
    19561 atgatttcca gcagttgcgc gttagtaaag ctggtatgtg cggggacatc tgccagaaat
    19621 tgccgcgtgt ctgttaccag ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag
    19681 tcatcccaac gttgggttaa cgcttttgct gatttctcca tttgatcctg atgcggttgt
    19741 gacgcctcaa cactgttcag cgcccgctcc gcagcctggg cggtcatctg cacaacatag
    19801 tacaaacgat cgcgcgcatc ggggatggct tccgctgctt cggcaatggc ctgatccagc
    19861 cccagctccc gcaaactgtc gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata
    19921 tcgccagctg aatgctcgtc agcaggtttg attgatggtt gcatcatagt cgcatcctca
    19981 catgcccagt ttctcaaaga ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg
    20041 cttcaccaca tagccactgg cccccgcttg cgccgcagca ataatgtttt ctttcttcgc
    20101 ttctgcagtc accattaaca ctggcaatgc tgacatcgcg ccatccgcac gaatcgtttt
    20161 cagcaattcc aggccatcca tgttgggcat gttccagtcg gagataacaa atccataacc
    20221 gcctgcctgc aacttattga gagcgtcgac cccatcttcc gcttcctcaa cattattgaa
    20281 tcctagctct ttcagcaggt tacgcactat gcgtcgcatg gtggaaaagt catccacaac
    20341 caaaaattta agttctttat ccgccatttc acactcctga tttaaatacg tatcgcctgt
    20401 ccggcactaa tttttgccag catttgctga cttacctggc taagatcgac cacttcgcag
    20461 acaccaccca tattgatggc ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt
    20521 tgcgcaaggg tccatgcccc cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg
    20581 cccataccgg ttaggattac cccaaccgca ttacgccccg cctgtttggc cacagaatgg
    20641 aacaacacat ctaccgatgg ccgatggcga ttaaccgccg ggccatcgtg aattttgatt
    20701 tgataatttg cgccactacg cgccagctcc atatgccgat cgcccggcgc gatataggca
    20761 tgtcccggca agacgcgctc tccgtcttcg gcttctttaa ccccgatctg gcaaagctta
    20821 ttaagtctgt cggcaaaaga gcgggtgaaa ccgggcggca tatgctgggt aattaacagt
    20881 gccggactgg aaagcggcaa cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt
    20941 gaagcaccaa tcgcaatcag tttttcagaa ctcaacaacg gccccgcctt cagcgttgtc
    21001 ggagccgaca atggcttatg tgctgcaagg ctcgcctttg ctgccgtacg caccttttca
    21061 gcaatcattt cgctatacgc cagcattcct tcgcgaatac ccagttgcgg tttggtgaca
    21121 aaatctatcg cccccagctc cagcgcgcgc agcgtgactt ctgacccttt gccggtcagg
    21181 gaagaaacca tcactacggg cattggacgc aaacgcatta atttttcgag gaaatccagt
    21241 ccgtccatcc gcggcatttc aacatccagc gtcaatacat cgggattgaa tttcttaatc
    21301 aaatcacgcg caaccagcgg atcaggggca gtcgccacca tttccatgtc gctatggctg
    21361 ttgatgattt ctgtcatgat ctggcgcatc agtgccgaat catcgacaga taacaccctg
    21421 attttgctca tcgttaatcc ttacttagcg catacaccgt ctgaccacgc agcgtgaagc
    21481 ggcgctcaag gtggctaaag ttttcagagt gacccgcaaa cagcaatccg tcgggtttaa
    21541 ggagcgaaac aaagcggcgc aaaatctcct gctgggtagt ttgatcgaag tagatcatga
    21601 cgttacgaca gaagatcgca tcaaacggcc ccggcacggt gtactgtttc gccagcaaat
    21661 tcagcggggc aaaatcaaca tagttcgcca gctcctgacg cacgcgtacc agcccttcat
    21721 gcggtcccgt ccctcgcatg aaataccgtt gcagttgctg cggcgtcagg tttttcaact
    21781 cttcatggcg ataaataccg cttctggctt tttccagcac ttcggtgtcg atatcgctgg
    21841 caaacacttt ccagcgtccg ggcgcggtgc ccaatgtatc agccagcgtc atcgctatgc
    21901 tgtacggctc ttcgccggtt gaagccgccg cgctccatac gcgatactcg ccagaacggc
    21961 gacgtgcgtg atccgcgagc agaggaaaat ggtgtgcctc acggaaaaat gccgtcagat
    22021 tcgtggtcag cgaattgata aacgcctgcc actcaccgct gtgctgatta gattccagca
    22081 agttcaggta atgaccgaaa tccgccagtc ccagcgcacg caaacgacga accagtcggt
    22141 tgtaaaccat gtcgcgttta tggtcagcca gaacgatccc ggctcgttga tagatcaatt
    22201 gacttatccg ccgaaaatgc gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata
    22261 aagacgtttg cccacagggc agagatgaag tcatagcgcc ttctcaatca cttcaggata
    22321 ccactggcgc aatttgtaac tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg
    22381 taaataccgc cacacgcgac gaaagatggt cggcctgatt cagtcgttgc aaatcgtcgc
    22441 gaaatgccca aaagacatgc cgttactgcc aatctgcaag atcccgcaga gaatcaaaat
    22501 taaaaacagc gtggtcgaaa ttcgaatacg attaaacatc aacgctcccc atcaggcggc
    22561 aatgaccgct ttagtaaata ctcgtcaaaa tgtttcccag tttggatctt gttcagcaat
    22621 tcgcagtcgt ggctgtgccg gtggttgctc actggcagga cgggatggtg tttgcggttt
    22681 attggtgagt gggctggctg ccagacggaa cgcggaaacc gcttgcgtta aacgactcgc
    22741 ctgttcttcc agcgcagctg cggcggcagc tgattcctgc accagcgatg cgttctgttg
    22801 cgtgacgcga tccatttccg aaacagccaa tgcgacttga tcgatgccac gactctgttc
    22861 atccgatgct gatgcaatct cgcccataat gtcagtcacg cgagtgacag cattgacgat
    22921 attgttcatt gtttcccctg cgctttcgac cagcaccgaa ccggtatcaa cgcgcgagac
    22981 ggagtcttca atgagggctt tgatctcttt tgccgcctgg gcgctgcgac tggcaagatt
    23041 acgcacttcg cccgccacca cggcaaaacc acggccttgt tcacccgctc gcgcggcttc
    23101 aacagcggca ttcagcgcga gtatattagt ctggaaagca ataccatcga taacgctgat
    23161 aatgtcggca attttcttcg aactatcggc gatctcatgc atcgttttca ctacgccatc
    23221 caccactttg ccgccgtgct gggcggtgtc ggaggcactt tgcgccagtt gcgaggcctg
    23281 gcgggcgtta tcggcgtttt gcttcactgt cgcggtgagc tgctccatgc tggcggcagt
    23341 ttcttccagc gcggatgcct gctgttcagt acgggaggaa agatcggtgt tgcccgccgc
    23401 aatttcacgg gtaccggcat agatggcatc tgaaccttcg cgcacatgag tgacggtgtc
    23461 agtcaaagag cgttgcatat gtgaaacgct ctgcgccagg tcgcccattt cactgcgccc
    23521 gtcaatggtc agggtattcg ccaggttacc accggcaatt tcgcgaatat gcgcaataat
    23581 ttttgccagc ggtgtaagca acatacggcg aatgccgtac cacgccacca gcagaatcaa
    23641 taccaccacc agcgcgataa ccgccagttg ccactgggca aatcggtaat catctgcgtt
    23701 gtcagtgacg atatcgcgat acagtttttc actgctgagg gcgtactgag caaacgcttc
    23761 gcccattgca ttttgcattc cctgggttgg ctgagcgaaa taagctccag tattgccata
    23821 atcaagataa tcaatcagtt cagttaacgc tgtgtgatag tttttatatt tttcatcaat
    23881 attacgactg gtagcgacca tttcaggtaa cggtgccatg cttttgaatt ttttataatg
    23941 agtcgcggcc tgggccaatg ttttcctggc gctatcgagc aattcaactt tggcattact
    24001 ttgctgattg gatgaatcca tcatcatccg caccgctgaa cgactcaggt taatgcgcgt
    24061 ttgcagcatt aaatcccatg ttgacgtcag ctcgccctgc tgttcccgta attgattgga
    24121 aaccacaaag ctcttctggc tatggtgaag ggaagaaaaa aacagactgc cggaaataag
    24181 ctgtaacagt gcgaataccc ccagcaccat taccaacagc gtgactacgc ggatacggtt
    24241 aatcataagg caccttcctg aaaacaagtt gatctcgtta tcggcaagga ggggggaaac
    24301 tttattgctg atgccacccg ccgcgaaatt gaaataaaaa acccgatgcg cagatcatcg
    24361 ggttcatttc aattgaggaa atcgggagaa ttacgccact tctgacgccg cgctatctaa
    24421 cagcgccatc tcttcgctgt tcagcagttt ttcgatgttc accagaatca acatgcggtc
    24481 gcccagtgcg cccagtcctg tgagatattc ggttgaaagc gtcacggcaa attccggtgc
    24541 cggacgaatt tgctccgccg tcaatgaaag cacgtctgag acgccgtcaa ccacgatgcc
    24601 gaccacccgc tgtccgagat tcaggacgat aactaccgtg ttgtcgttat agtccacatc
    24661 cacctggctg aacttaattc gtaagtcaac aatcggcaca ataacgccgc gcagattcgt
    24721 gacgcctttg ataaacgctg gcgtgttcgc aatccgtgtc acctgatcgt agccacggat
    24781 ctcctgcact ttcagaatat caataccgta ctcttcatca ccaagggtaa ataccagaaa
    24841 ttcctggcct gacggctcgc tggccagctt tgttacattc gtcataccgg tcatattgtt
    24901 acctttttac tcattcaggc ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc
    24961 aaggcggaga catcaacaat cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa
    25021 atgccgggga ctttgcgata gttactttca aggtttttaa ccacaacctg gtgttgacca
    25081 attaattgat ccaccagcaa ggcgtagcgg cgaccgccac tttgtaagat caccacaatt
    25141 ccctgggtgg cttcggtttt cgcgcccgcg acgttgaaca ctttccacag ttcgacgatg
    25201 ggcagatatt caccccgcac ttccagcacc cgctcgccgc cagccagtgg atgcagatcg
    25261 gcttcacggg gttgcagtga ttccatgaca gcattcagcg gcagaatgaa aacttcatcc
    25321 gcaacgcgta cggacatgcc gtcaagaatt gccagcgtca gcggcagtaa aatgcggatc
    25381 gtagtgccag taccctgcat cgactggatc tcgacatgac cgcccatctc ctggatatta
    25441 cgtttaacga cgtccatgcc gacgccacgc ccggagacgt cggtgacctg ctctgccgtg
    25501 gagaagccag gtgcaaatat cagcattgca acttcgtcgt cgctcatgtt ttcgctgacg
    25561 gtcagacctt gcgaggccgc ttttgccaga attcgctcac ggtttagccc cgccccatcg
    25621 tcggtcactt caatgcaaat gttgccgccc tgatgttcgg cagacagaat taaatttccg
    25681 acgctgtttt tacctgcagc gagccgtttt tctggcagtt caataccgtg atcgaggcta
    25741 ttgcgtacca ggtgggtcag cgggtcgata atgcgttcaa tcaggctctt gtcgagttca
    25801 gtagaactgc ccaccagcgt cagttctacc tgcttgccga gttttcccgc cagatcacgc
    25861 accagccggg gatagcgact aaagacatat tccatcggca tcatgcgaat cgacatcact
    25921 gattcctgca aatcacgagc gttacgttgt aactgcccca tgctggtaat caaatcacca
    25981 tgattaaccg ggtccagttc actggagcgc tgagcaagca tggactgggt gatgaccagc
    26041 tcgccgacga ggttaattaa ttgatcaacc ttttctaccg ctacacggat gctggtggat
    26101 tcactgctgc gcgtcgtttt ttcccgctcc acgcgaccgg ttggcgcttg ttcggctgcc
    26161 agtttaagca ctggtggggt ggatattttt ggcgagacgt ctactgtttc aaaggtaatc
    26221 tgatcggctt caatcacaaa acagagtacc gctgtgatgt catcttcggc gatgtcgccc
    26281 ggtaatattg ccgagagaga atccgcccct ttcaccacgt cagttaacgt tgtcagatgc
    26341 cccaactctt cttccagcag gtcgacttcg ccggccttca ggcgcgaaag gataattcgt
    26401 cgcggtgact gactacgact ctgctcatct tgcggttcac ttttggcaac cacacttaat
    26461 cgggtcactg cagatggcgt ttcgcctttc gcttctaatg ccagttgacg caaggcctgg
    26521 cagatataat cgaagctggc ggcatccggc tcttgcgact gtttataagc gtcgagctgt
    26581 tcttgcatga tgtccttcgt ttccaaaaac agattgataa tgtcggtgtt gagttgcatc
    26641 tcacctcgtc tggcttcatc gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg
    26701 ctgaagccaa aggttcctgc ccctcctttg atcgagtggg cagcccgaaa gatggcattc
    26761 aactgttcgg catctggcgc ttccggctgc aaaaccagca gatgctgctc catatcagcc
    26821 aacagttcgt ccgcttcatc aaaaaatgtc tgataaaaat cgcttatatc catgctcacg
    26881 ctgtcacctc ggttcggctg atggcattgt gggaacactg acctgtggtg caacctcagg
    26941 tttttccagg gcgcttactg gctcattctg gctttcggcg ttttcatgca aaatggcctg
    27001 ttcggcgtgt ttgttcagta ccagcaggct gatgcgacgg ttgacggcat catcaggacc
    27061 gcgatcgctt aagcgcatcg ttgccgccat gccgacgaca cgtaacactt tgccgccatt
    27121 caacccgcca accatcagtt cgcggcggga tgcattggcc cgatcggcag aaagctccca
    27181 gttgctatat cctttctcac cgctggcgta agggaaatca tcggtatgac ccgaaaggct
    27241 aatacggttg ggaataccat tcagaacggg cgcgatggcg cgcagaatgt cgcgcatata
    27301 gggttccaca tcggcactgc cggttctaaa catcgggcga ttctggctat cgatgatctg
    27361 aatacgtaga ccttcctgga ccagatcgat tttgagatgt ggacgtaacg cccgcagttt
    27421 cggatcggac tcaatcaact ggtcgagatc gccacgcaat ttccgcaatc gactttgctc
    27481 catgcgtttt ttcagctctt cgatgttcgg ctgcttattc acttccccct ggctttgggt
    27541 gtaatcatca cccccaccgg gaattgggct ttcactatta gaaatgcgat cgccacccgt
    27601 aaccgctgtc gccagcggag tccggaagta ctccgcaatc tgaatcagct cttttggact
    27661 ggagatagag atcagccaca tcaccagaaa aaaggccatc attgcggtca taaagtcggc
    27721 ataagcaatc ttccacgatc catgtgctgc tccgtggctt ttggctttgc gtcgtttgac
    27781 gacaataatc ggatgcgctt gattcttcat gcttcctcgg ttgtcgtctg ttgttgcgga
    27841 tttttcaccg cacgcacatg ctcttccagc tcaataaacg acggacgttc gctggaatag
    27901 agcgttttgc gaccaaactc aacggcgata ggaggtgcgt aaccgttcag atttgaaagc
    27961 agagtgactt tgacgcactg catcattttg ctggtttcgg cgcttttctg acgtaaaaca
    28021 ctcgctaatg gggaaataaa tccgtaagcc aataaaatac cgaggaaagt ccccaccatc
    28081 gcatgagcga taagcgcccc cagctcggcg gcaggacgat cggctgaacc taacgcgtga
    28141 actaccccca ttacagccgc aacaatacca aacgccggaa gtgagtcccc gaccagcgcc
    28201 agactgttcg ccgggacttc tgcctcgctt tcgtgcgtct caatctcttc atccatcaga
    28261 gcttcgattt cgaaggtgtt catgtgaccg ctgataatca ggcgcagata atcgacgata
    28321 aattcaagca tgacgctatc cgcaaggatg cgtgggtagc tggcgaagat ctcgctctca
    28381 cggggatttt caatatcgcg ttccagcgaa aacatcccca tctgccgcga tttcgccatc
    28441 aaccgataaa gcagagccag cagatccata tacattgctt tggtgtattt ggagcgacga
    28501 aacagcaacg gcagcgcctt cagcgtgcct ttaatcgctt tgccattatt gccgacgata
    28561 aacgacccaa tccctgcacc ggcaataatc accagttcag cgggttgata gagtgctcca
    28621 aggcttccac cggtcatcaa ataaccgccg aaaactgtac cgagaacaac caggtaacct
    28681 aataagataa gcacgacatc atccttccac tgttgaccat gacaggatgt tcagtcgtca
    28741 ggcgttaaca cgcgataggg gcaaaaaaaa gcagcggtac atcgttaccg ctgctggaat
    28801 gtttcgccac accgtatcag ttaaacagcc tgtactctct gttcatccag cagttgtggg
    28861 ataatatcgg caggattctg ggaaagttta cgtcttttta ctgcccggga tggcggctga
    28921 cataagctgc aggcaaagct gccaacaggc tggtgagcat gggtaataaa attgccgccg
    28981 cagcagttgc agttggaaag ttgcagtaat ccactttcaa caaaccgcac caatgtccag
    29041 gcacgggtta atgccagcag cggtccttct tctgcttgcg ggcactgttc aaggtataaa
    29101 cggtaggctt tgatcaccgc atcgacgcca ttacacaagc cagttttcag taaaaactgc
    29161 catgcattac agaacatcga agcatgaacg ttttgttccc aggtcataaa ccagtcggtt
    29221 gagaatggca gcatgccttt cggcggtggg cttccgcgca gttctttata gagttttatc
    29281 agacgtccgc gacttaactg tgtttcgctt tccagcatct gcaaacgagc gcccagggtg
    29341 atcaattcca ttgccagctg aatatcccgc gcttcctgaa caatgctttt ttcactcatg
    29401 atcaggccct tttcttgcgc agcgcttctt caggctgatt aacatcattc agcaagcgag
    29461 ttgagagcat gatgccggta tgaatttgct ggagatcgtc aacgcgggaa tcttgcgtca
    29521 actgagtaat cgtctggtgg ctgtcaaaac ggaagtgaca aaccagttga ttggtttctg
    29581 ccagcttaac catttgcgga agagtcagtg ccgctaacgt tgtcgccatt tcttcattta
    29641 tgccgagacg aaacatagcg gacgctttgt cctgaacaat cagacgctgt gcaagtagta
    29701 aatatgacaa gttgatgtca taaatgtgtt tcagcaactc ggaggtatgc attattccca
    29761 cccagaataa ccaactttat ttttatgcgg tctcaccgca ccccgtgatg tcgccgggaa
    29821 gccccggtaa aaaataatta gcattagaat agttgcgata agctgcaata agcagaccca
    29881 cctttttggt ttaataagtc cttacaaata gaagggggtc tttacactta tctaagattt
    29941 ttcctaaatc gacgcaactg tactcgtcac tccacgcaca tacaacggag gggggctgcg
    30001 attttcaata atgtgtgatg cagatcacat aaaacgctca attacttaac ataaatgtat
    30061 aagtcatact tttgttttgg gtgtatttca atctgttaaa aaatttttcg ctacactggc
    30121 aagcaaaaat gaaacaggaa taatcgaaat gggatgttgc gcacagttaa aataactcac
    30181 cgtaaataat catctgctat aaataatcgc tttaatgcaa taccagataa gccattttta
    30241 aacaaacact taccgcacaa caaactaatt aactacaaag aaaggcaaca atcaacctaa
    30301 tgttaataaa atgttttaca tatattaacc acttgttcaa t
//
LOCUS       DADSZD010000039        30170 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.039, whole genome shotgun sequence.
ACCESSION   DADSZD010000039 DADSZD010000000
VERSION     DADSZD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.039"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(78..2525)
                     /gene="glgP"
                     /locus_tag="J1Z28_001593"
     CDS             complement(78..2525)
                     /gene="glgP"
                     /locus_tag="J1Z28_001593"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBA7716436.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2544..3977)
                     /gene="glgA"
                     /locus_tag="J1Z28_001594"
     CDS             complement(2544..3977)
                     /gene="glgA"
                     /locus_tag="J1Z28_001594"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBA7716437.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3977..5272)
                     /gene="glgC"
                     /locus_tag="J1Z28_001595"
     CDS             complement(3977..5272)
                     /gene="glgC"
                     /locus_tag="J1Z28_001595"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBA7716438.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5290..7263)
                     /gene="glgX"
                     /locus_tag="J1Z28_001596"
     CDS             complement(5290..7263)
                     /gene="glgX"
                     /locus_tag="J1Z28_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBA7716439.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIYG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7260..9446)
                     /gene="glgB"
                     /locus_tag="J1Z28_001597"
     CDS             complement(7260..9446)
                     /gene="glgB"
                     /locus_tag="J1Z28_001597"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBA7716440.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYCHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9719..10822)
                     /gene="asd"
                     /locus_tag="J1Z28_001598"
     CDS             complement(9719..10822)
                     /gene="asd"
                     /locus_tag="J1Z28_001598"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBA7716441.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11014..11607
                     /gene="yhgN"
                     /locus_tag="J1Z28_001599"
     CDS             11014..11607
                     /gene="yhgN"
                     /locus_tag="J1Z28_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBA7716442.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11664..13004)
                     /gene="gntU"
                     /locus_tag="J1Z28_001600"
     CDS             complement(11664..13004)
                     /gene="gntU"
                     /locus_tag="J1Z28_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBA7716443.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13008..13535)
                     /gene="gntK"
                     /locus_tag="J1Z28_001601"
     CDS             complement(13008..13535)
                     /gene="gntK"
                     /locus_tag="J1Z28_001601"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBA7716444.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13674..14669)
                     /locus_tag="J1Z28_001602"
     CDS             complement(13674..14669)
                     /locus_tag="J1Z28_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GntR"
                     /protein_id="HBA7716445.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14762..15457)
                     /locus_tag="J1Z28_001603"
     CDS             complement(14762..15457)
                     /locus_tag="J1Z28_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBA7716446.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEILLFDLPPV"
     gene            complement(15580..16617)
                     /locus_tag="J1Z28_001604"
     CDS             complement(15580..16617)
                     /locus_tag="J1Z28_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA7716447.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITNGAPNYVKESEVLTNLEILERGFE
                     QASPSAVTLAR"
     gene            16951..17439
                     /gene="yhhY"
                     /locus_tag="J1Z28_001605"
     CDS             16951..17439
                     /gene="yhhY"
                     /locus_tag="J1Z28_001605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7716448.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17500..19233)
                     /gene="ggt"
                     /locus_tag="J1Z28_001606"
     CDS             complement(17500..19233)
                     /gene="ggt"
                     /locus_tag="J1Z28_001606"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBA7716449.1"
                     /translation="MIKPTFLRRVAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLISKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKIWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            19353..19793
                     /locus_tag="J1Z28_001607"
     CDS             19353..19793
                     /locus_tag="J1Z28_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBA7716450.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19780..20523)
                     /gene="ugpQ"
                     /locus_tag="J1Z28_001608"
     CDS             complement(19780..20523)
                     /gene="ugpQ"
                     /locus_tag="J1Z28_001608"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA7716451.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAK
                     RCRKHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20520..21590)
                     /locus_tag="J1Z28_001609"
     CDS             complement(20520..21590)
                     /locus_tag="J1Z28_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
                     /protein_id="HBA7716452.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLPLNGGYRQYAGRKM
                     TLGIRPEHIALSLQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            complement(21592..22437)
                     /gene="ugpE"
                     /locus_tag="J1Z28_001610"
     CDS             complement(21592..22437)
                     /gene="ugpE"
                     /locus_tag="J1Z28_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBA7716453.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22434..23321)
                     /gene="ugpA"
                     /locus_tag="J1Z28_001611"
     CDS             complement(22434..23321)
                     /gene="ugpA"
                     /locus_tag="J1Z28_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBA7716454.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23419..24734)
                     /gene="ugpB"
                     /locus_tag="J1Z28_001612"
                     /pseudo
     CDS             complement(23419..24734)
                     /gene="ugpB"
                     /locus_tag="J1Z28_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(25131..25844)
                     /gene="livF"
                     /locus_tag="J1Z28_001613"
     CDS             complement(25131..25844)
                     /gene="livF"
                     /locus_tag="J1Z28_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBA7716455.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25846..26613)
                     /gene="livG"
                     /locus_tag="J1Z28_001614"
     CDS             complement(25846..26613)
                     /gene="livG"
                     /locus_tag="J1Z28_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBA7716456.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26610..27887)
                     /locus_tag="J1Z28_001615"
     CDS             complement(26610..27887)
                     /locus_tag="J1Z28_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivM"
                     /protein_id="HBA7716457.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASEIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRSAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27884..28810)
                     /gene="livH"
                     /locus_tag="J1Z28_001616"
     CDS             complement(27884..28810)
                     /gene="livH"
                     /locus_tag="J1Z28_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBA7716458.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMLGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28858..29967)
                     /gene="livk"
                     /locus_tag="J1Z28_001617"
     CDS             complement(28858..29967)
                     /gene="livk"
                     /locus_tag="J1Z28_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBA7716459.1"
                     /translation="MKRNAKTIIAGMIALTISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINDKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLTLVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
BASE COUNT         7291 a         8400 c         7569 g         6910 t
ORIGIN      
        1 ttattgtgta tagaaatacc gtgagttcca ctctgtatta cttcagcgga acacacggta
       61 tttagccgta tttgaactta caatctcacc ggatcaatat gccagatatg atcggcgtac
      121 tctttgatcg tacggtcaga agagaagtag cccatattgg caatgttcag catcgctttt
      181 gcggtccact cttcctgacg ctcgtagagt tcgtcgactt tatcctgaca atcgacataa
      241 ctgcgataat ccgccagtac ctggtagtga tcgccgaagt tgatcagcga atcaaccaga
      301 tcgcgatagc gacccggatc ttccggactg aatacaccgc tgccgatttg cgtcagcacc
      361 tgatgcagct cctcatcttt ctcgtagtat tcacgcggtt tgtagccctg acgacgcagt
      421 tcttccactt cttccgctgt gttaccaaag ataaagatat tgtcagcacc gacatgatcc
      481 agcatctcga cattcgcacc gtccagcgta ccgatagtca gcgcgccgtt aagcgcaaac
      541 ttcatgttac tggtgccgga agcttccgtc cccgccagcg aaatctgttc agacagatcg
      601 gccgccggaa tgatcaactg cgccaggcta acactgtagt tcgggatgaa cacgactttc
      661 agcttgtcgc caatctgcgg atcgttgttg atcactttcg ctacgtcatt gatcaaatga
      721 ataatgtgct tcgccatgta ataggccgaa gccgccttac cgccaaaaat attcacgcgc
      781 ggtacccact tcgcatccgg gtcggccttg atgcggttat agcgggtgat cacgtgcaac
      841 acattcatca attgacgttt gtattcgtga atacgtttga tttgcacatc gaacagcgct
      901 ttcggattta ccaccacatt cagctgctgg gcgatatact ccgccagacg ctttttgttc
      961 tccagcttcg cctgatgcac cgcgtgatta accattggga aatcacagtg ttgttgcaga
     1021 tcattaagca ggctaaggtc ggtgcgccag ttgcggccca ggtgttcgtc caacacggct
     1081 gaaagcgatg ggttcgctac cgccaaccag cgacgcggcg tcacaccgtt ggtgacgttg
     1141 gtgaaacgac ccgggaagat tttcgcaaag tcggcaaata gcgattgcac catcaggtta
     1201 gagtgcagct ccgatacacc gttaactttg tggctcacca caaccgccag ccaggccata
     1261 cgcacacgac gaccgttgga ttcatcaatg atcgacgccc gtcccagcag atcggtatcg
     1321 ttcggatact gttcctgcaa ggttttcagg aaatagtcgt tgatttcaaa gatgatctgc
     1381 aggtgacgcg gcagaatttt acccagcata tcgactggcc aggtttccag cgcctcgctc
     1441 attagcgtgt ggttggtgta ggagaagacc tgacaacaca cttcaaacgc gtcgtcccag
     1501 ctaaattggt gctcatcgat cagcagacgc atcatctcag gaatcgacag caccggatga
     1561 gtatcgttga ggtgaatcgc aattttatcc gccaggttat cgtaggtttt atgcaactga
     1621 taatggcggc ttaaaatgtc ctgaatggtc gaggaaacca ggaagtattc ctgacgcagt
     1681 cgtagctcac gcccggagta ggtggagtcg tccggataca gtacgcgaga tacgttctcg
     1741 gagtggtttt tatcttccac tgccgcgaag tagtcgccct ggttgaattt accgaggtta
     1801 atttcgctac tggcttgggc actccacaaa cgcaacgtgt tggtcgcgtc ggtgtcgtaa
     1861 cctgggatta tctgatcgta agctactccc agaatctctt cggtttcaat ccagcgcgtt
     1921 tttttacctt cctgctgaat gcgaccgcca aaacggactt tatagcgcgt gttgtggcgt
     1981 ttgaattccc acgggttacc gtattccagc cagtagtcag gcgactcttt ctggctaccg
     2041 ttaacgatgt tctgcttgaa cataccgtag tcatagcgga tgccgtaacc gcgccccggc
     2101 aaccctaacg tcgccagaga atcgaggaag caagccgcca gacggcccag accaccgtta
     2161 ccgaggcccg ggtcattttc ttcatcaatc agctcttcga gatttaatcc catcgcttcc
     2221 agtgcgccct gtacatcttc gtaaattcct agcgacaaca tggcgttgga aagcgtacgg
     2281 ccaatcaaaa actccatcga caggtagtaa acctgacgag tttcttgcga caactgggca
     2341 cggtttgagc gtaaccagcg ctccacgaga cgatcgcgca cagcaaataa cgttgcgttc
     2401 agccattcat gtttattggc gacgaccgga tccttgccaa tcgtaaacat cagcttgtaa
     2461 gcgatagagt gcttaagagc ttctacgcta agcgtgggcg atgaatatgt aaacggagca
     2521 ttcatatagg cgtttcctga aaactatttc aagcgatagt aaagctcacg gtacgacttc
     2581 gccgcgacct gccagctaaa atccattgcc atagcctgac gttgcacaaa ccgccacagt
     2641 gaaggacggg accacagtac aaaagcacgt cgaatagccc gtaacagcga ccaggcatta
     2701 ctatcttcaa agacaaaccc actggcgacg ccatctgcaa ggttctcaag agaacagtca
     2761 gaaaccgtat cagcaagccc accggtgcgc cgcactaacg gcagcgtacc gtacttcaat
     2821 ccataaagtt gcgttaagcc gcacggttca aaacggctgg gcaccagaat gacgtccgcg
     2881 ccgcccataa tgcgatgcga aaatgcttcg tgatagccaa tctgaacgcc cacctggccg
     2941 gggtattccg ctgccgccgc aaggaaacct tcctgcagca ccggatcgcc cgcgccgagt
     3001 agcgccagct gcccgccctg ctccagaaga ccaggtaagg cttccagcac caggtcgaga
     3061 cctttctggc tggtcagacg gctcaccact gcaaaaagcg gcactttatc gtcaacctta
     3121 agccccattg cgatttgtaa ctggcgctta ttttccgctt tatcttccaa cgtatcgcgg
     3181 gtgtaacgcg aggccaacag taagtccgtc tctggactcc agattttctc gtccacgccg
     3241 ttcagtacgc cggaaagacg cccttcacgg tgacgctgtt gcaacagacc ttccataccg
     3301 taggcaaact gcggttcggt gatctcgcga gcgtaggttg ggctgactgc cgtaatgtga
     3361 tcggcatagt acagaccggc cttcaggaaa gagatttgtc cgttgaattc cagcccatga
     3421 atattaaaga atgaccatgg caattggatg tcattcatgt gatgtgcata aaacatgcct
     3481 tgataggcca agttgtgcac agtaaacacc gacttcgccg gacgcccgcg cgccgccaga
     3541 tacgcaggcg caaggcctgc atgccagtcg tgcgcatgca ccacatcagg acgccagaat
     3601 gggtcaagcc cgctggccat ttctgcccca acccacccca gcagcgcaaa acgcaatacg
     3661 ttgtcggtat aggcaaataa gttggtatcg tgatacgggc ttcccggacg atcatagaga
     3721 tgcggcgcgt caatcaggta tatgccaacc ccgttgtaat gaccgaacaa aagcgtgata
     3781 tgtccggcga aggtatcacg acgggatact acctgcgcat cggtcacgcc acggcgaata
     3841 tcgggaaatg caggcaacag tacgcgagcg tcaacgccgt ctgcgatttg tgctgcgggt
     3901 aatgccccaa taacatcagc cagaccgccg gttttaagca gcgggaacat ctctgaacat
     3961 acatgtaaaa cctgcattat cgctcctgtt tatgccctaa cttccgtagc atttcgcgcg
     4021 ttaccagcac gatgccttct tctgaacgat agaaacgacg tgcatcttcc tctgcgtttt
     4081 caccaatcac catgccttcc ggaataacac aagcacgatc gatgacgcag cggcgcagac
     4141 ggcacgagcg acctacccat acttccggta acaatacggc ggaatcaatg ttgcagaatg
     4201 aattcacgcg aacgcgcgag aacagaacgg actgcaccac caccgaaccg gagatcacac
     4261 aaccgccgga taccagtgag ttaagggtca tcccgtggct accggagcga tcctgcacga
     4321 atttcgctgg cggtaatgat tcattgtagg tgcgaattgg ccaattgcga tcgtacatat
     4381 ccagttccgg caccacagag gccagatcga ggttcgcttt ccagtaagct tccagcgtac
     4441 ccacatcgcg ccagtacggc tcggcatccg ggtcggattg tacgcaagag agcgggaacg
     4501 ggtgcgcata ggccagaccg gcttcggtga tcttgggaat caaatctttg ccaaagtcgt
     4561 ggctggagtt ctcatcgcga tcgtcttctt ccagcagttc atacagatag tcggcgtcaa
     4621 agacgtagat acccatactc gccagagatt tgctcggatc gttcggcatt gacggcgggt
     4681 tagctggttt ttcaacgaat tcgataattt tatcgttctc atcaaccgcc ataacgccaa
     4741 acgcggaggc ttcttcaatc ggtactggca tacaagcaac ggtgcaacgt gcgccttttt
     4801 cgacgtggtc gataagcata cgcgagtagt cttgcttgta gatatggtcg cccgccagga
     4861 tcaccacgta ttccgcttta taacggcgga taatgtcgag gttttgggtg accgcatctg
     4921 cggtgccgcg ataccagttt tcccctttca ttctctgctg tgctggcagc agatcgacaa
     4981 actcgttcat ttcttcattg aagaatgacc agccgcgctg gatgtgctgc accagagtgt
     5041 gggactggta ctgggtgatc acgcccatac gacggatccc ggagttgatg cagttagaca
     5101 gcgcaaagtc gataatgcgg aacttaccgc cgaagtgtac ggccggtttt gctcgcttat
     5161 tggttaaatc cttcaggcgg gtaccacgtc ctcccgccag tatcagggca acagatttca
     5221 atggcagctg gcgcgccaac attaagtgat cgttcttctc taaactaacc atgactaact
     5281 ccttttttat catctctgga acacacacaa tccgtgtgca ggtccctgcc agacagccgt
     5341 aatcactggg ttatcctctc cagcgaatgg gggaatggcg tgccactccc cagcaggtaa
     5401 aacaatctct gttacctcaa gcgtggcatt aattgcgatc aaaaagcgat ccgagagcag
     5461 aatttgcagc tgtttcggcc cgttttgcca ctcatccgtg cttaaaggtt gagcatatcg
     5521 atttagccaa cggacattgc cgtcgccttc ttcccaccag cgattctcca tcaaagcggg
     5581 aatgcgcttg cgcagatgga ttaacgcggc ggtaaatgcg gttaaaccac tgcttgcctg
     5641 cgaccagtcc aaccaggtta attggttatc ctgacagtag gcattgttat tgccatgctg
     5701 gctgtgacca tgttcgtcac cggccagtaa catcggcgta ccctgggaga gcaacaacgt
     5761 tgttaacagg gcgtgaatgc tgtcgcgccg ccgttcaacc agatcaagag taccgcctaa
     5821 cccttcttta ccatgattgt tactgtaatt gttgttggtc ccgtcgcgat tttcttctcc
     5881 gtttgcttca ttgtgtttat ggttgaagca aacgcagtcg cgaagcgtaa aaccgtcatg
     5941 cgcggtgacg agattaatcg cggcactcgg cagacgacca ttacgtttaa aaacatcgct
     6001 ggaggcagca aaacgcccgg caaacgctcc cagaggtaaa tcataatgca gccagaaacg
     6061 acgggctgca tcgcggaaat gatcgttcca ctcggcaaac agcggcggga aatttcccac
     6121 ctgataacca ccaggagcga tatcccacgg ttcagcaatt aacttcacct gcgagagcac
     6181 cgggcagttc tggatagcgg taaacaacgg cgcatcctga cgaaactctg gcgtacggcc
     6241 catgactgcc gccagatcaa agcggaaacc atcgacgtgg caggtttcta cccaataacg
     6301 caggcaggcg ctggcataat ctaccaccgc cggatgactc aaattgagcg tgttaccaca
     6361 accggtccag ttgtgataat cgccgtcttc tcttatccaa taatagctac ggttgtcgat
     6421 cccacgcagc gagaataacg ggccgtcgag gtccagttcc gcactatggt tgagcacgat
     6481 atcaagaatg acttcgatac ccgctttatg cagtgctttg attgcatcgc gaaactcatc
     6541 cagcgccgtt tctggcgagc aggcatacgc cggatgcagc gcaaacatcg ccaccgggtt
     6601 gtacccccag tagttactaa gccccatgcg ttgcagacgt ggttcgctgg caaattgtgc
     6661 caccggcagc aactccagcg cggtaatgcc caactgtttc aaatagttga tcatcaccgg
     6721 atgccccagg gctttataag tgccacggat ctcgaccgga atctccgggt gcaggtacgt
     6781 taatcctttg acatgggctt cataaatgat ggtgctgccc cacggcgtgc gcggcggagc
     6841 atcatcttcc cagtcatagt gatcaaccac cactacgcat ttcggcgcaa tggcggcgtt
     6901 gtcgcgatag tcaggttcat tatgaccggc gtgcagcagc gggttatctt taaactcccc
     6961 gtaaatttgc cgcgcgcaag gatcaatcaa caacttcgcc gggttaaagc gatgcccctc
     7021 ggcaggttgc caggggccat gaacgcgata accataacgc aaacccgggc gcgcatccgg
     7081 cagataaccg tgccaaatgt cgccactgtg ccctggcaag tcatagcgat gttcctggcc
     7141 attggcatca aagacacaca gctctacccg ctcggcatga gcggagaaaa gtgtgaagtt
     7201 gacgccctga ccgtcgtaat gcgcgccgag gggagtgggt ttgccaatgg cgagttgtgt
     7261 cattctgcct cccgaaccag ccagatagtg gccagaggtg gtagcgtcag gctcagtgaa
     7321 tgctgacgac cgtggctggc aatctcatcg ctgtgtactg cgccgccatt gcctgcatta
     7381 ctgccgtgat agtgcatgga atcggtattg aggatttcac gccatttacc cggctggtta
     7441 ataccgaagc gataatcatg acgcggcacc ggtgtaaagt tactggcaac gatgatttcg
     7501 ttaccctctt tatcgcgacg cacaaagatc agcaccgagc gttctttgtc atccaccacc
     7561 agccattcaa agccgtacgg gtcaaaatcc agttcatgca ttgctttatg gtggcagtag
     7621 gtgtggttca gatcgcgcac cagacgctgg acaccgtggt gccagttatc gccgccttcc
     7681 aacagatgcc agtcgaggct ggcgtcatgg ttccactcgc ggccctgggc aaattcgttc
     7741 cccatgaaca gtagtttctt gcccgggaat gcccacatcc agccgtagta ggcgcgcagg
     7801 ttagcgaatt tctgccatgc gtcgcccggc atccggtcga gaatcgattt tttaccgtgg
     7861 accacttcat catgcgacaa cggcaggacg aagttttcag tgtagttgta gagcatcccg
     7921 aaggtcagtt tatcgtgatg atactgacga taaatcgggt cgagcttcat gtagtccagg
     7981 gtgtcatgca tccagccgag gttccacttg taccagaagc ccagaccgcc catatcctgc
     8041 ggacgagaaa cgccagggaa atcggtagac tcctccgcca ttgtcactgc accggaaacc
     8101 tgctcaccaa gaatacggtt ggtattacgc aagaattcaa tcgcttcaag attctcgcga
     8161 ccgccaaatt cgttcgggat ccactccccc tctttacggc tgtagtcgcg ataaatcatt
     8221 gacgccaccg catcgacgcg cagcgcatca ataccaaaac gttcaatcca gtaaagtgcg
     8281 ttaccgacaa ggaagttact gacttcacgg cgaccatagt tgtagatcag cgtgttccag
     8341 tcctgatgat agccttcgcg cggatcgctg tgttcataca agttcgtgcc atcaaattcg
     8401 gcaagcgcaa agtcatcggt cgggaagtgg cctggcaccc agtcgagaat cacgttcaga
     8461 ccagctgcgt gtgcggcatc aatgaaataa cggaagtcgt cgcgagtacc aaaacggcgg
     8521 gtcggtgcat acaggccggt tggctgataa ccccaactgc catcgaaggg atgctcgtta
     8581 atgggcagta gctcgagatg ggtaaagccc atccatttag cataaggcac cagttgatcg
     8641 gccagctcgc ggtagctcaa ccagaaattg ttgtcggtgt ggcgacgcca ggagccaagg
     8701 tgaacttcat aaatagagat tggcgcatca aactgattcg cttttttgcg ctcttcagtc
     8761 tgcacaacct tttccggcag cccgcaaata agagacgcgg tttccgggcg catttgcgct
     8821 tcgaaggcat aagggtcgga cttcagacgc aagttgccat tggcatcaat catctcgtat
     8881 ttatagagct gaccgttatg cgccccaggg ataaacagtt cccagattcc gctctcttta
     8941 cgcagtcgca tcggatgacg gcgaccgtcc cagtagttga attgcccaac caccgagacc
     9001 cgacgggcgt ttggagccca gacagaaaaa cgcgtacctg tgacgccatc catagtatct
     9061 gcatgcgcgc ctaaggtttc atacggacgt aggtgagtac cttcagataa tagccaggca
     9121 tccatttcct ggattagcgg gccaaaacgg taaggatcat caatcaggtt ttgctgacca
     9181 tgccagacaa cagccaactg atagcggaaa aaattcttac gtcgcggaat gacaccgcta
     9241 aagaatcccc gtgagtcgag acactccagt tttgcgagtt tgcgcccggt tttcggttca
     9301 atcacccaca catcggtagc gtcgggtaaa agggcacgga cttccagtcc cgcggtggtt
     9361 ttatgcatcc ccagtacgga aaaaggatcc gcaaaatggc ctgcaattag cgcgttaatc
     9421 acgtctctat cgatacgatc ggacatgctt gtcttcctgt tttattgtgt caccccatcc
     9481 aactgaattt ttgacttctg gttgtgacat ttttttgacc tgaacggcgc agcactctgt
     9541 gcatcctctc tgcgtcgtcc tcacttcagg taaggctgtg aatactcatg tattcagcca
     9601 cccttaaaga atagccaatg ctctatttaa ctcccggtaa atcatgaaac atctgcgctt
     9661 actcctgtat tacgcactaa caggggcggc atcgcgcccc agatttaatg aataaagatt
     9721 acgccagttg acgaagcatc cgacgtagcg gctccgcggc cccccacagc agctggtcgc
     9781 ccacggtaaa ggctgacagg aactccggtc ccatattcag cttacgcagg cggcctaccg
     9841 gcgtggtcag cgtgccggta acggcagctg gggttagctc acgcatagtg atttcccgat
     9901 cgttcggaac aactttcgcc cacggattgt gcgcagccag cagttcttcc acggtcggaa
     9961 tagacacatc ttttttcaat ttaatagtga atgcctggct gtggcagcgc aatgccccga
    10021 cacgcacaca taaaccatct accggaatta cggaagatgt gttgaggatc ttgttggttt
    10081 ccgcctgccc tttccactct tcgcggctct gaccgttatc aagctgtttg tcgatccacg
    10141 gaatcaggct acccgccagc ggcacgccaa agttatctac cggcagctca ccgctacggg
    10201 ttaaggttgt gactttgcgt tcgatatcga gaatagcaga ggacgggttc gcgagttcat
    10261 ctgccacatg gccatacaga tggcccattt gggttaataa ctcacgcata tgtcgcgcac
    10321 caccgccgga agcggcctgg taggttgcaa cggacaccca atcaacaaga tcattggcga
    10381 ataaaccccc cagcgacatc aacatcaggc ttacggtaca gttaccgcca acaaaagtcc
    10441 tgatgccatt atttaatccg tcggtaatga cgtcctgatt gacggggtca agaatgatga
    10501 tggcgtcatc tttcatgcgc agagatgatg ctgcgtcaat ccagtaacct tgccatccgc
    10561 tttcacgaag ctttggatag atttcgttgg tataatcgcc gccctgacag gtcacaatga
    10621 tatcgagggc ctttagcgcc tccagatcaa aggcatcctg aagtgtgcca gtggttccgc
    10681 caaaagacgg cgcagcctga ccaagctgag aagtagaaaa gaagacaggg cgaatggcgt
    10741 cgaagtcgcg ctcttcaacc atgcgttgca tgagaacgga gccgaccata ccgcgccagc
    10801 cgataaaacc aacatttttc ataagcgttt tttcctgcaa agatgtgtgc tgtataaatg
    10861 tgccggtctc ctcctggcac atctttcacc atacaaaaag cagccaaagt cgcaagtgaa
    10921 attaatcaat gatagcgaag ccatcagtaa tgcgacttat cctgctttgt tagcacgcag
    10981 aaagtccgcg gcaattatca gggaatttga gttatgaatg aaatcatttc tgcaacagtt
    11041 ttattgatcc tgattatgga tccgctcgga aacctaccta ttttcatgtc cgtactgaaa
    11101 catactgaac cgaaaagacg gcgggcaatc atggtgcgag agttgcttat tgctctcctt
    11161 gtgatgctgg tgttcctgtt tgcgggcgag aaaattctgg catttcttag cctgcgggca
    11221 gaaaccgtct ccatttctgg cggcatcatt ctgtttctga tcgccattaa gatgattttc
    11281 cccagcgctt caggaaatag cagcgggctt ccggcaggtg aagagccatt tatcgtgccg
    11341 ttggcaattc cgctggtcgc cgggccgact attctcgcca cgctgatgtt gttgtctcat
    11401 cagtacccga atcagatggg acatctggta attgctctgc tactggcctg gggcggcacc
    11461 tttgtcatcc tgctacagtc ttcgctattt ttacgtctgc taggcgagaa aggggtgaac
    11521 gcacttgaac gcctgatggg attgattctg gtgatgatgg caactcagat gttcctcgac
    11581 ggcattcgaa tgtggatgaa ggggtaacga ggagagtttt tcgaaaagag cccggtagcg
    11641 tcagaactac cgggtaaaca aacttaactc aacagctgga acgcaatcat cccaacgatg
    11701 gcaccgacag tgccgaggat ggtttccatc atggtccagg ttttaagcgt ttcggcttcg
    11761 gtcgcgccgg taaatttacc gaacaaccag aaacccgcgt cgttaacgtg gctgacaaca
    11821 atcgaaccac cggcgataca aatcgacagc gccgccattt gcgcaccgga gtagttcagt
    11881 tgttcaatga ccggcatcac caatcccacc gccgttaaac aggcaacggt ggcagaaccc
    11941 tgaatgatgc gcactgcagc tgccagcacg aagcaggtga tggcaatcgg caggcccatg
    12001 ccggttaacg cttcgcccag tgccggaccg acaccagagt caaccagcac ctgtttgaac
    12061 acgccgcccg caccaatcac cagcagaatg atccccgccg gttgcagcgc gtgaccgcaa
    12121 atctccatca ctttgtcttt tggcataccc tgacgcattg ccagaccgta aatcgccacc
    12181 aggcaagcaa ccagaatcgc ggtaaacgga tggccgataa actcaaacca ttcgtaagcg
    12241 gtagaacctt ctggcacaaa acgcgcggca atggttttca gccccaccag caccagtggc
    12301 aacaggatca gcgacaggct gaatccgaag gacggcattt tgccttcgcc gagatgcggt
    12361 tcgctgatgt cgtcaggaat atgcaactcc acgtagcggc tgatgaaatt accccacagc
    12421 ggcccggcaa taatcattcc cggaattgcc gcgcacaggc caatcaggat catccagcca
    12481 aaatcggcat tcatctgcga tgccagcagc attggcgctg gcccaggcac caggaacgca
    12541 gccgctgccg ccacgcctgc aaataatggg attaccagct tcaccaggtt cgtgccggtg
    12601 tggcgcgcca ttgagaaagc aacgctaatc agcagaacaa tcgccacttc aaagaacagc
    12661 ggtagcgcac aaaccagccc cgcaaggccg atagcataat gcacgcggct gtgaccgaag
    12721 gatttgagca ttttgacggc aatctgatcg accgcgccgg tttcatgtaa gatcttacca
    12781 aacatggctc ccagggcgac aaccaccgcc aggaagccga gggtgcctcc catccctttt
    12841 tccatcgtcg ctgcgatttt atcgagcggc ataccagaaa aaaggccagc ccccatggac
    12901 accaccatta aagccaggaa agcgtgcata cgcgccttca tgactaaaaa cagcagcagt
    12961 aaaacagacc ctactgctgt taaaacaagc gttaatgtag tcactactta tttgcctttt
    13021 ttaataacct caatggtgct tgccacaaca ccttccagcg gttgatcaat atccaccacc
    13081 agtacatcgg tttcgtccgc acccggctcc tgcagcgttt caaactgcgt caccagcatt
    13141 tgggttttaa agaaatggcc tttgcgcgct ttcaggcggc tttcaatcac atcaaaatcg
    13201 cctttcaggt agatgaaaga gagattagga ttaccttcac gcagcaagtc gcgatagtgt
    13261 tttttcagtg cagaacagac gatcagcgat actttattgg tgcgctgcat agcaaacgcg
    13321 gcgtcgttca gcgcctgcaa ccacggtttg cgatcgtcgt cattcagcgg ttcgccagac
    13381 gccatttttt cgatattgca gcgcggatgg aggaaatcgc catcaagaaa cgcggcatga
    13441 agttgatgcg ccacttcact ggcgaccgca gatttgccgc tgcccgatac gcccatcaag
    13501 acgtaaatgt ggtgatcatg gttagtcgtg ctcaaagtgg tgcccccaca atacaagaat
    13561 taaaaatgtt acgggtaact gttatcggta acattgtcca gccggacaat atcagaagca
    13621 atatccatcc gtgccttaag tgtataagtg tgagctactt caaatttgta ggcttaaata
    13681 gatccgcccg gtgacaaggt gaaacctaaa tctaacattt tcggtgtcac agattcgcca
    13741 cgaatacgcg ccagcaggcg ctcagcgcca atgctgccca tccgctcacg cggcgtcagc
    13801 acactcgcca gccgtggctc catcacctga ccaatgtcat gaccgtggaa accggcaatc
    13861 gccatatcgt caggaacttt taaccccaga cgctgacatt caaacgccgc gccgaccgcc
    13921 aggtcatcat tagtacagaa cacgccatcc agctgcggat attcccgccg cgcctggcga
    13981 atcagttcaa taccggaaga gtaagaagaa gattgctcaa ccatcacgct atacggcacc
    14041 aggcctgcat ccagcatcgc ctgttcgtat cccttctgtt tgatgatagt acgttcgtcg
    14101 aggcgtgcgc cgagataggc aatgtgacga tgcccgcgag caataatggc ggtggtcatc
    14161 tggcgtgccg cttcaaagtt atcaaaaccg acggcgatat cgaggcacgg cgacttactg
    14221 tccatcaact ccaccaccgg aatccccgcc acttcaatca tctttaaggt acgcggcgtg
    14281 tgggtacgtt cggtgaggat caggccgtcg atattccatg agagcattga ttcgaggcgt
    14341 tcttgctcca tttccggttt ataaccgtag tgcgccagca tggtttgata accgtgcgcg
    14401 tcggtgacgc tttcgattcc gcgtaatact tccgcgaaaa cctggttggt gagagaaggt
    14461 aacaggacgc caatcgcccg gctggtggcg ttagagagta tatcgggcgc gcgattggga
    14521 atataaccca gttcatcaag agcggcggca atcttgccgc gtagagcgac ggaaacctgc
    14581 tccgggttgc gtaaaaaacg gctgaccgtc attttggtca cgcctacacg gtcagccaca
    14641 tcctgaagta cgggtctttt ctttttcatc gtcctgaagg tacaaaagag atagattctc
    14701 ttagtttaac acggacgcat cagaaccttc ccgattcctc aggaaggttt gtgagataaa
    14761 tttagactgg cggtaaatcg aacagcagga tttcactgtc gctatcagca tggatggaaa
    14821 tcgcctgctc atcccagatt gccagaccat cgctggtcga ggctttcacg ccgttaatgg
    14881 tgacattgcc tttcaccacc tggatccaga cgcggcgttc agcggcaatc tgatgcaccg
    14941 actgctcatc tttcaccagc gcccaacgat acagttccat atcctgatgc actttcaatg
    15001 aaccatcacg cgcatccggc gaaagcacca gttgtttgcc ctgcacggca tcgaagcgac
    15061 gctgttcata acgcggcgta atgccgtttt cttcaggcat gatccagatc tgatacagat
    15121 gcagacgctc ggtgctgctt gggttgtact ctgagtgacg aatacccgta cccgcactca
    15181 taatctggaa ctcgcccgcc ggaacctgct ctttattgcc catgctgtcc tgatgctcaa
    15241 cagtaccttc cagcacgtag gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc
    15301 cctgccctgc ttcaatcacg tcgtcgttaa tcacgcgcag cgcagagaag cccataaaat
    15361 tcggatcgta atagttggca aaagagaaag tatgccagga gtccagccag ccatgatttg
    15421 catgaccgcg ttcatttgct ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt
    15481 tcgatgggct tagtgtggac ccgatccgcc tgggatgata gagggtgaaa attgacccct
    15541 ctgttcaaaa aaattgaact attcgagggg ccatcaaact tacctcgcga gagtcactgc
    15601 ggagggagaa gcttgctcaa aaccgcgttc aaggatttcc agattcgtaa gaacttcaga
    15661 ttccttgacg taatttggcg caccgttggt gatggtttgg tacaacgcat cataaacgcg
    15721 cccataatcg cccatctctg gcttcatctc ttctcttacc gtcacgccct cgtcattgac
    15781 atactccagc acaccgaccg aatcatccgc tgcgaatcct ggttcgcccg gcataatatt
    15841 agccttcagg ctggtttcct gctggtcgat accgtattta ataaacgaac ctttcttacc
    15901 gtgaacgata aatttcggat aatcgatttt caccagatgg ctggttttga cgatggcttt
    15961 tagatcgcca taaaacagct gcgcttcaaa ggtgtcgtcc ggattggctt tattacgcag
    16021 gctgcggatg tcataagcga cgtgatccgg gcgaccgaac agagaaataa tctggtccat
    16081 cgtatgcaca ccaaggccat agaacgcgcc atcctgcggt agccccggtt tggtttctgc
    16141 caccgggcgg taatagtcaa aatggctttc cacttcgaca atctctccca gcttgccgct
    16201 ttcaatcgct tttttcgctg tcaggaagca ggaatcaaag cgacgattct gatacggcgt
    16261 aacgatcagc cctttgcttt tcgccagtgc aaacagctct ttcgcctgcg caattgtcgg
    16321 agtgaacggt ttttcgacca gcacattttt cccggcttcc agcgcgcgct tcgcgtactc
    16381 gaagtggctg tccgcgtggg tgcagacgac aaccagctta acatcgggat cgtttagcac
    16441 ttcgtcgaga tcgctggtga aatggatatg ggaataaatg ggggcctgtt cttccggctt
    16501 cgcatggcgg cgaaagatat gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg
    16561 cagatggtaa cgggtggtgc ttttgccgaa gccaataaag gcgcagttaa tgaccatgat
    16621 ttcgtccttt ttaaggtggt tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg
    16681 agtgcgcata acgaacacaa gcacttcccg aggataaatt gagaacgaaa ggtcaaaaaa
    16741 aaagccagca cccggctggc taagtaatac tggaagcaat gtgagcaatg tcgtgctttc
    16801 aggttctccg cgagggtctt cctgatcgcg agacaataat aatcattctc attcgcactt
    16861 gtccaacact ttttgcaaaa aaatgcattt gactcgcatt tgaaagtcaa tgatgttgaa
    16921 agggacattt accccaaaga ggacaaagga atgagtgaga tagtaatacg ccacgcagaa
    16981 acacgcgatt acgaggccat caggcagatt cacgcccagc cggaggtgta ttacaacacg
    17041 ctacaggtgc ctcatccttc agaacaaatg tggctggaac gactcaccgc tcgccctggc
    17101 atcaagcagt tagtcgcctg tattgatgga atcgtcgtag gccatctcac cattgacgtg
    17161 caacaacgcc cacgccgcag tcatgttgcc gattttggta tctgtgtcga ctcccgttgg
    17221 aagaaccgcg gcgtcgccag cgccctgatg cgagagatga ttgaaatgtg cgacaactgg
    17281 ttgcgggtag atcgtattga gctaacggtg ttcgtcgata acgcgccagc aattaaggtc
    17341 tataaaaaat ttggctttga gattgaaggg actggcaaga agtacgcgct gcgtaatggt
    17401 gaatatgtcg atgcgtatta tatggcgcgg gtgaaataga gaatttgcaa tctacgtttg
    17461 tcatttaagc cctcacctac cgggagaggg cttaagatat taataccccg ccgttaaatc
    17521 atccaccgag cgcgggtcgg atgcaccgta cagctcaccg tccggcccaa ccataatgct
    17581 ttgtgtactg cccatcgcct ctttcagcgc cactttctga ccttttgctt ccagcagctt
    17641 gatcgtatcc gggctaaacc ctttttcgac acgcagctcg tccggcaacc actgatggtg
    17701 gaaacgcggc gcattggtcg cttcggcgac gttcattcca taatcgatgc tattcaccac
    17761 catttgcagc acagtggtga taatccggct accgcctggg cttccggtga ccagccagat
    17821 tttaccatcc ttgaccacaa tggttggtga catcgacgac agcgggcgtt tattcggccc
    17881 gacggcgttg gcatcgccgc ccaccagccc gtaaacgttc ggtacgcctg gtttggcgga
    17941 gaaatcatcc atctggttat tgagcaaaat accgctctcg cccgcgacaa tgcccgtacc
    18001 gaaggtggtg ttcagcgtat aggtcaccgc cacggcgtta ccgtctttat ccactactga
    18061 gtaatgggta gtttgattac tctcataagg cgcaagcttg ccaggacgaa tttcgctgga
    18121 tggcttcgct ttattgatat cgatttgatc ggcaatagat ttggcatagg ctttattggt
    18181 cagcgcctgc cacggtactt tgacaaaatc cgggtcgcca agatattccg agcggtcggc
    18241 ataggcgtat ttctcagctt ctgccatgat ttgcatcgcg tcggcgctgc caaagccgta
    18301 cttctgcata tcgaagtttt ccagaatatt gaggatttgt acgatatgaa taccgccgga
    18361 agatggcggt ggcatggagt aaacctgata cccgcgataa tcgccgctta tcggtgtgcg
    18421 ttcgaccgct ttatatgccg ctaaatcttc tttgctgatc aagccaccgt ttttctgcat
    18481 ctcctgggca atctgttccg ctatcgtgcc tttgtagaat tcatccgggc cgttttcggc
    18541 aatcatctcc aggctctttg ccaggttcgc ctgcaccagc ttgtcgccct ttttcagcgg
    18601 ttcgccctct ttccagaaga tagctttgct gttttcgtgg ttcggcagca cttcactgcc
    18661 gtaggttttg agatcgtcag ccagcgcgtc gttaacgata aaaccatcgc gtgccagttt
    18721 aaacgccggt tgcaccactt tattcaacgg catggtgccg tatttatcca gcgccaacga
    18781 gaaacccgct accgtaccgg gtgtgccgga agccagatgc gaagtgagtg attttttgct
    18841 gtccgggttg ccctgatcat cgaggaacat atcgcgggtc gctttggcgg gtgccatttc
    18901 gcggaaatcg atagccgtgg tattgccatt tttcgagcgg atcagcataa aaccaccgcc
    18961 gcccagattc cctgcctgcg gatgcgttac tgccagcgcg tagcccaccg ccacggcagc
    19021 atcaacggca ttcccgccct ccttgaggat atccaccccc acctgagtgg cagtagcatc
    19081 tacagacgcc accattccct gcttagcgcg taccggatgg aagacatctt cctccacacc
    19141 ataagaaaca ggtggcgcgg ctaccgtact aaaacaactt cctgtgagca gagcagcaat
    19201 ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat taaggggcaa
    19261 ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgccggg aagcgagtaa
    19321 acttaaagaa tatcttcaga ggaggatacg cgatgaaacg acttctgctt ttgacggcac
    19381 tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct aaccagccgg
    19441 gatatcagat ccccagccag cagcggatgc aaactcaaat gcagacgcag caaatccagc
    19501 aaaaagggat gctgaaccag caactgaaaa cgcaaacgca gctgcaacag cagcatttag
    19561 aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg gagcgaaatc
    19621 ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc aaccgtaatc
    19681 cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc gacatgctga
    19741 atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg taaagttcgg
    19801 accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca gcaactctgc
    19861 cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcat ctttcaactg
    19921 catcactcgc gctttatcga gcaacttatg attgagatga atagagacgc agcccagtcg
    19981 cgcggtcaat tctcgccagt cgtcgcgcca ttcatcaagc aataaaccac gcggcagttc
    20041 cggtgctgcc tgttgcgcgg cttctaaagc atcaatctca aacgatgaca gcagcggcgg
    20101 cgtcataccg gtccacagtt cgcgtgccgc caacgccacc gttttgcccg ttaatggtcc
    20161 agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgtttgc gacaacgttt
    20221 cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt accaactgcc
    20281 tgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc agccgttgct
    20341 ggtgcgttca agattgtcgt catggagcag gaagatctcg ccatctttcg ataactttgc
    20401 gtcaaattcg atcatcttat gaccgtatct tgccccgacg tcgattgccg ccagagtgtt
    20461 ttccggggcc agcttaccgc caccacgatg ggcgacgatg cggggataag gccagttact
    20521 catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgg cagatgcagc
    20581 cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac cagcttctgt
    20641 tcgccccaac gtccgtgcgc caggttatct gcaccgagga tctccagcgt gtccatcacc
    20701 agcggcacac caccttctgc ctgcaagctt agcgctatat gttccgggcg aatgccgagg
    20761 gtcattttac gcccggcata ctgacggtag ccaccgttta gtggcagcgt aataccgcca
    20821 tccagttcga aatgcgtacc ttcgttattc acgcggccag ccagtaggtt catcgccggg
    20881 ctgccgataa aactcgccac aaacaggctg gcgggctttt cgtacacttc aactggcgtg
    20941 ccaatctgtt cggcgacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc
    21001 tcaacctgat cgtgggtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc
    21061 agttcaagac gcatctgcac acgcaacttg gcatcgaggt tagagagcgg ctcatcaaac
    21121 aggaacactg ccggctcacg cacaatcgca cgccccatcg ccacacgctg acgctgaccg
    21181 ccagatagct cacgcgggcg gcgcttaagc aggccgtcca gctccagaat gcgcgccgct
    21241 tctttaacac gttcggcaat ttgttgcttg cccatgccgc gaattttcag cccccacgcc
    21301 atgttctcct ctacgctcat atgcggataa agcgcgtagt tctggaacac catcgcaatc
    21361 ccgcgatctt tcggctccat ttcggtgaca cgcttgcggt cgatccagat atcgcccgtc
    21421 gttacccgct ccagtccggc aaccattcgc aataacgtcg atttcccgca gccagacggc
    21481 ccgaccatca cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctgg
    21541 gttttgccat cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc
    21601 actatcgacc aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg
    21661 gatcagggtt aacaacatcg ccgccatcac cgagttccat tccgttgtgc cttcgcctgt
    21721 agcgatcatc cctttaatcc ctgccacggt ggtgccgaga tcaacgtcgg taataatcaa
    21781 caacggccac aaatactggt tccagccgta aataaaggta atcacaaaca gcgccgccag
    21841 attggttttg gacagcggaa aaacgatgtc gcaaaagaaa cgcattggcg acgcgccgtc
    21901 gatccgcgcg gcttccacca gttcatccgg tagggtcata aagaactggc gaaacagaaa
    21961 cgtggcggtc gccgaggcca tcagcggcag cgttaaacct gcgtagctgt cgagcatttt
    22021 cagattggcg atgacttcca ccgtcgggaa gatacgcact tcaaccggca gcatcagggt
    22081 gataaaaatc atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa
    22141 tgccgagagc atcgagacgg taattttgcc gagcgtaatg ctgaacgcca tcacaaagct
    22201 gttaagcaac atccgccaga acggcgcgct attcgtgcct accccgttta cccagatgtt
    22261 gtggatgttt tccagcagat gtgtgccggg gattagcgtc atcggtgcgg catagacttc
    22321 ttgtttatcc agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat
    22381 cccgaggatc agcatggtat ggctgaatat tgtcagccac ggacggttct caatcattgg
    22441 taacgtacct tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac
    22501 atcaacacca ccgactgcgc ggcagacgaa gccaaatcca gtccggtaaa gccttcacgg
    22561 tagatcttat aaatcagcgt cgtggtggcc tgtactggac cgccggacgt ggcggcgtcg
    22621 atcaccggga aggtgtcgaa gaaggcatac accaggttca ccaccagcag gaagaaactc
    22681 accggagcga taagcggcag cgcaatctta aagaagcggc gaatcgggcc agccccatca
    22741 atggctgcgg cttcgatcaa cgaacggggg atggattgca gcgcggcata gaagaacaga
    22801 aagttgtagc tgatttgctt ccacactgag gcaaacacca ccaggaacat cgcctgaccg
    22861 ctgttttgcg cgtggttcca gtcgtagccg aactcagcga gaaaatgagt aatcagcccg
    22921 cgaccggggt taaacaggaa gatccacaat accgcggcaa cggcgggagc cacggcatac
    22981 ggtagcaaca tcaaggtttg atagaaacgg ctgccgcgca cgatgtactc caccagcgct
    23041 gcgaagaaca gcgataccag caaaccgctg acggtgacaa aagtgctgaa ttttatcgtc
    23101 gtccagaagg cgtcgagata gtagctgtcg tgaaacagcg tgacgaagtt atccaggccg
    23161 acaaactggc tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg
    23221 cccgcaggcc agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcaaataa
    23281 ggcagccagc gcgagcggaa caccggacga gatgatgaca ttacagttaa ttcctgaact
    23341 gtgccggatg cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg
    23401 catccgacat tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt
    23461 cccacgctca acggcagtat ccagcgcctg ctgtggtgtc ttcttaccgg tccacacgct
    23521 ctccagctct tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gcagcccttt
    23581 ggtgaacggc aacggcggct tgttcagcat ctgacgcgtg gcaatatccg ctcctgggtt
    23641 tttctcgtaa aagccctgct cacgggtcag gtcatacgcc gctttggtga ttggcagata
    23701 gccggttttc tgatgccact cggcagcgtt ttctggtttt gcgaggaagt cgaggaactt
    23761 cgccacgccg gtgtaggttt ctttatcttt accctgcatt acccatagac tggctccgcc
    23821 gataatggcg tttgtggcgc gtctttcgca tcggcgtcgt aaggcatcat gcctacgcca
    23881 tagttaaatt tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtagtcatc
    23941 gcgcaatcac cgttatagaa cttctcagtg gattcatcct tacgcccaac gtagctgaaa
    24001 tcgcccttct tgttcatctc ttcgagcatg gcgatgtgtt tcacctgctc cggcttgttg
    24061 aactcaagca ccgcgtctgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg
    24121 ttccaggcgc taaagttttc cagttggatc catccctgcc agccgctggc gtagccgcac
    24181 ttcatgccgg aggcttttag ttttgcggca tagtccgcca gatcctgcca ggttttcggc
    24241 ggttgttccg ggtctaatcc tgctttcttg aaggcgtctt tgttgtaata gagaacgggg
    24301 gtcgaactgt tgaacggctg ggagagtaag tggccggttt tgctgtcgga gtaatagcct
    24361 gaaaccgtcg gcacaaactg cgactcatcg aattgaatcc ccgcctcttt aaatacgtca
    24421 tacaccggtt taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc
    24481 aaaatagccg gagcgttgcc ggtacgaaat gcggcaatcc ccgcgcttaa attctgttcg
    24541 tagttgcctt tataggtcgg tacaattttg taatcagggt tttcggcgtt aaaacgttgg
    24601 gccagagaat ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc
    24661 gtcactgcct gtgcattccc cattaacgcc agtccgagcg tcagtgctga agctgtataa
    24721 tgtaacggtt tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta
    24781 tgctcgcggg gtaacatgac atgctcgaat tacagaaaaa taactatttt gttacatttt
    24841 taagatagta aggtgtcaga aagatgacaa ggcggtgacg gcgcgggcga gggaaaatgg
    24901 gagatggggc acggataagc ggaaaaatag aaggtctgaa tcaaactcta cagattactc
    24961 atcgtttcat gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc
    25021 aatatattgc aactcaccca gtaggcctga taagcgcagc gcatcaggca attttacatt
    25081 tgtcacctgt ctcaaaggag tcttttgact ccttatcaat caacgtgtta ttacccgcct
    25141 aaatatgcac ttctcactgc ttcattcgcc agcagcgcgt cgccggtatc ggagagcact
    25201 acatgaccgt tttccagcac gtagccgcga tccgccagct ttagcgcctg gttagcgttc
    25261 tgctcgacaa ggaagatggt catcccctgc tcgcgcagtt gttcgatggt gtcgaaaatt
    25321 tgctggatga taatcggtgc cagcccgagc gatggctcat caagcaacaa caaacgcggg
    25381 ttgctcatca gcgcgcgacc aatcgccagc atctgctgtt caccgccaga catagtgccc
    25441 gctcgctgaa cacggcgctc atgcagacgt ggaaacagtt catagaccca ctttatgcgc
    25501 tcctggaact ggtcgcgttc ggcaaaaaag ccgcccatcg ccaggttctc ttccaccgtc
    25561 atccgcgaga agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc
    25621 gccgtctgcc agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc
    25681 ggatcgccgc ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgagg
    25741 gtgacaatct cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg
    25801 tagtgggcgc tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa
    25861 ataggcacgg atcacgtccg ggttattacg gatctgctcc ggtgtaccgt ttgccagcgg
    25921 cgtcccctga ttgaccacgt aaattctgtc tgaaatcccc atcaccagct tcatatcgtg
    25981 ttcaatcaac aagatagtgg tgttgtgatg gttacgcagt tcggcaatca gctcatccag
    26041 ctctttcgtc tctttcgggt taagacctgc tgccggttcg tcgagcatta aaatctccgg
    26101 ctgcgttacc atacagcggg caatctccag acgacgctgg tcaccatagg ccaggttact
    26161 cgcctgacgg ttggcatgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc
    26221 gagcgcttcg ctctgggcgc gacggaaaga gggggttttc aacaggccgg agaacagccc
    26281 agttttcagt tgctgatgct gtgccaccag caggttttca atcaccgtca tttcacggaa
    26341 cagacgtaca tgctggaagg tgcgcaccac gcccatgcgg gcaatttgct gccccggtaa
    26401 accttccagg tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttat agaatccggt
    26461 caggcagttg aagaccgtgg tttttccggc accgttaggg ccgattaacg agacgatctc
    26521 ctgcgggtac agttcaagat tgacgttgtt caccgccagc aggccgccga agcgcatcat
    26581 caggccgtta acagataata atggctgact catgcctgct ctccttttgc tgcgccgttt
    26641 ttcagcttga gttgcgggcg cgtcatgggc agcaagccct gcggacgcca gatcatcatc
    26701 agcaccatca aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaactcg
    26761 cgcgatacca ccagcaaaac agccgccaga atcactgcaa attgcgagcc cataccgccg
    26821 agcaccacta tcgccagcac aaacgccgat tcggcaaaag tgaaggattc agggctgaca
    26881 aagccctggc gcgcagcaaa caacgttccg gcaaaaccgg caaacgcagc gctgatggta
    26941 aaggcagtca gcttgatacg acgcgggctt aagcccagcg aacgacaagc gatttcatct
    27001 tcacgcaacg cttcccacgc acgccccagc ggcatccgca gcaggcggtt aatgacaaac
    27061 aggcttaaca ccaccagcag caacgccacc aggtagagga aaatgacgcg atcggaggga
    27121 tcgtatttca ggccaaagaa attactgaac gtgtcccagc cgccttcacg ggcgctacgg
    27181 ctgaactcga gtccgaagag cgtcggtttc ggaatctgac tgataccgtt cggtccgccg
    27241 gtaatttcgg tgttattgag cagcaatatg cgcacaattt cgccgaaacc gagggtaacg
    27301 atcgccagat agtcaccgcg cagacgcaac accgggaagc cgagcaggaa gcccgccgcc
    27361 gccgccatca acccggcaat cggcagacag gtccagaagc ccagaccgta atagtgattg
    27421 agcagcgcaa aagtgtaagc gccgatggcg taaaagccgc cgtatcccag caccagcaga
    27481 ccagaaagac caaccaccac gttcagcccg agaccgagaa taatgtagat catggtcaga
    27541 gtggcgatat ccaccgtccc acgcgacacc ataaatggcc acgccaccgc aagcaccagc
    27601 agcgccacga ggaacagttt ctgcttcacc gtggagccat caatcgcggg cagaataaac
    27661 ttcggcccgg aaacgctttt tagccctttc tggaaagccg gtcgcaaaag ctggaagaaa
    27721 aagaccaccg ccgtgccgat aaacacccac tgccaacgga tctccgaagc cgtgtcgacc
    27781 accagtttgg tgccatccag ctccagttgc acgcccataa agacgcccgc caggacaaag
    27841 aacatagcgg cagagagcag cgccattgca atatgcatcg gtttcatact ttctctacct
    27901 ccgggcgacc cagaataccg gtcggcatca ccagcagcac caggatcagc agggcgaatg
    27961 agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca
    28021 ggatcagccc gccaatcatc gcccccggaa tgctgccaat cccaccgagc accgcagcgg
    28081 taaaggcttt catcccggcc ataaagccga tgtaagggtt aattacgccg tagaactgac
    28141 cgagcagcac acccgccacc gccgccatcg ccgcgccaat cacaaaggtc agcgcaatca
    28201 cccgatcggt gttgatgcca agcaggctcg ccattttcag atcttccgcg caggcacgac
    28261 aggcgcgccc catgcgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaaag
    28321 taacaatcca tatcaccgcc tgcatggtgg taatagacgc agagaagttt tcgctatggc
    28381 ccaccaccca ctgaccgtta aaaaggctgg gcagcgccac atcgcgcgaa ccttcggtca
    28441 ggctgacgta gttttgcagg aagatagaca tgccgattgc cgagatgagt gcaatcaggc
    28501 gcttagagtt acgcaccggg cgataggcta cccgttcgat actccagccg taggcgctgg
    28561 caatgacgat tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca
    28621 gcatcatcag tgcggcaatg atcataaagg agacgtagct gccaatcatg taaacctcgc
    28681 cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca
    28741 gcgcgtaggt actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact
    28801 gctcagacat aaggtaacct ttctaaaccc gcccgttttt cacgaacggt gggatgatca
    28861 cttggctgcc gtggatgaac cgtcggcatg ccactggaag acaccaaaat cgaatccctt
    28921 aagatcgcct ttttcatccc agttcagcgg cccaattacg gtgtttgcac cgttagcttt
    28981 taaatctttc accagcgtca gcggctcatc gctgccggta cgttcaaggg cagttgccag
    29041 agattgcacc gccgcgtagg tgatccagac atagggcccg gacggatctt tcttgtcggc
    29101 tttcagcgca tcaacgatgc cctgatttgc cggatcctgg tcatagcgtt ttggcatagt
    29161 gaccaacatg ccttcggcag catcgccagc aatattcgac aacgacgcat tacccacacc
    29221 ttccggcccc ataaactggg ttttcaggcc aacggaacgg gcctggcgca gcatctgccc
    29281 catttccggg tagtaaccgc cgtagtaaac gaagtcgatg ttttcttttt tcaggcgggc
    29341 gatcagcgcg gagaaatctt tctctccagc ggtaatgccg tcgaagaaga cgacgttggc
    29401 gttagccgct ttcagcccgt cctgcaccga acgtgccagt ccttcgccat actgttgttt
    29461 gtcgtgaatg atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt
    29521 tggcccctgg gaagagtcca gcccggcagt acgcatgatg tattgataac cgcgttgggt
    29581 cagctccgga ttggtcgctc ccggcgagat catcagaata ccttcgtctt catagatatc
    29641 tgatgcaggt tgggtagagg aggaacacag atgaccaata acatatttaa tgccgtcgtt
    29701 aacgattttg ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac
    29761 gccaaccagt ttatcgccct taattccccc tttgtcatta atgtctttaa ttgcctgacg
    29821 cgcgccatta aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac
    29881 ggcgacttta atatcgtcag ccatagcggt gtgtgaaatt gtcagtgcaa tcatccctgc
    29941 gatgatagtt ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc
    30001 tttgttttta tgtgttaaca aatcaaactg ttcttttttt atactgcact gtttttacct
    30061 gtctgattaa gggtttagcg cagcattttg tgatgatagc gattaaaatc cctatttttc
    30121 agtcaattaa gaacagataa tattctgaat ttattgatag ataaacagaa
//
LOCUS       DADSZD010000040        30118 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.040, whole genome shotgun sequence.
ACCESSION   DADSZD010000040 DADSZD010000000
VERSION     DADSZD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.040"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(543..1433)
                     /locus_tag="J1Z28_001618"
     CDS             complement(543..1433)
                     /locus_tag="J1Z28_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBA7716460.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRATAAQQAGLKLIVGLNADPEFFIH
                     QKQSSAALESYLNRLLAADLQQARLWSAVPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAQSILEY"
     gene            complement(1434..4406)
                     /gene="nfrA"
                     /locus_tag="J1Z28_001619"
     CDS             complement(1434..4406)
                     /gene="nfrA"
                     /locus_tag="J1Z28_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBA7716461.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDDRLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     TPLFTTDAQEKSWLYLLSKYSGNPVQALANYTVQFADNRQYVVGATLPVLLKEGQYGA
                     AQKLLATLPANEMLEERYTVSVATHNKAEALRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPDAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRLGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(4393..6630)
                     /gene="nrfB"
                     /locus_tag="J1Z28_001620"
     CDS             complement(4393..6630)
                     /gene="nrfB"
                     /locus_tag="J1Z28_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBA7716462.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDKVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMINRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLDNDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(6780..8222)
                     /gene="cusS"
                     /locus_tag="J1Z28_001621"
     CDS             complement(6780..8222)
                     /gene="cusS"
                     /locus_tag="J1Z28_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBA7716463.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDVRGTRFVIILPA"
     gene            complement(8212..8895)
                     /gene="cusR"
                     /locus_tag="J1Z28_001622"
     CDS             complement(8212..8895)
                     /gene="cusR"
                     /locus_tag="J1Z28_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBA7716464.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            9052..10434
                     /gene="cusC"
                     /locus_tag="J1Z28_001623"
     CDS             9052..10434
                     /gene="cusC"
                     /locus_tag="J1Z28_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBA7716465.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            10458..10790
                     /gene="cusF"
                     /locus_tag="J1Z28_001624"
     CDS             10458..10790
                     /gene="cusF"
                     /locus_tag="J1Z28_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBA7716466.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLEGKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            10806..12029
                     /gene="cusB"
                     /locus_tag="J1Z28_001625"
     CDS             10806..12029
                     /gene="cusB"
                     /locus_tag="J1Z28_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBA7716467.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKCVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            12041..15184
                     /gene="cusA"
                     /locus_tag="J1Z28_001626"
     CDS             12041..15184
                     /gene="cusA"
                     /locus_tag="J1Z28_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBA7716468.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVSEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            15286..16668
                     /gene="pheP"
                     /locus_tag="J1Z28_001627"
     CDS             15286..16668
                     /gene="pheP"
                     /locus_tag="J1Z28_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBA7716469.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASTLNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(16825..18072)
                     /locus_tag="J1Z28_001628"
     CDS             complement(16825..18072)
                     /locus_tag="J1Z28_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HBA7716470.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(18180..18833)
                     /gene="nfsB"
                     /locus_tag="J1Z28_001629"
     CDS             complement(18180..18833)
                     /gene="nfsB"
                     /locus_tag="J1Z28_001629"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBA7716471.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(18912..19295)
                     /locus_tag="J1Z28_001630"
     CDS             complement(18912..19295)
                     /locus_tag="J1Z28_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBA7716472.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            complement(19360..19608)
                     /locus_tag="J1Z28_001631"
     CDS             complement(19360..19608)
                     /locus_tag="J1Z28_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBA7716473.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(19674..20792)
                     /locus_tag="J1Z28_001632"
     CDS             complement(19674..20792)
                     /locus_tag="J1Z28_001632"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBA7716474.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDTVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWVGE"
     gene            21238..21390
                     /locus_tag="J1Z28_001633"
     CDS             21238..21390
                     /locus_tag="J1Z28_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA7716475.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(21512..22132)
                     /gene="entD"
                     /locus_tag="J1Z28_001634"
     CDS             complement(21512..22132)
                     /gene="entD"
                     /locus_tag="J1Z28_001634"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBA7716476.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSAQTARELTDNIITPAEHKRLADCGLAFPLALTLAFSAKESAFKASEI
                     QAAQGFLDYQIISWNKQQIIIRLEDEQFAVHWQIKEKIVITLCQHD"
     gene            complement(22307..24547)
                     /gene="fepA"
                     /locus_tag="J1Z28_001635"
     CDS             complement(22307..24547)
                     /gene="fepA"
                     /locus_tag="J1Z28_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBA7716477.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            24790..25992
                     /gene="fes"
                     /locus_tag="J1Z28_001636"
     CDS             24790..25992
                     /gene="fes"
                     /locus_tag="J1Z28_001636"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBA7716478.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQASLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGVLLEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALYAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25995..26213
                     /locus_tag="J1Z28_001637"
     CDS             25995..26213
                     /locus_tag="J1Z28_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HBA7716479.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            26210..30091
                     /gene="entF"
                     /locus_tag="J1Z28_001638"
     CDS             26210..30091
                     /gene="entF"
                     /locus_tag="J1Z28_001638"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBA7716480.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPSLLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDIPNLTSLCYNAPLTPQGSAPLQFSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLSGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTSANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMTPEQAWAPWIAEL
                     DIYRQDCAHVDIISPEYFKEIGPLINTQINN"
BASE COUNT         7005 a         7690 c         8147 g         7276 t
ORIGIN      
        1 acaatgcgct gtcagttttt tagtttaatt gtctgtttgt gttttgcatt gtggttttat
       61 gggctaatta tttttttctt tgtggagttt ttatctattc aagtgccatg cctttagatg
      121 catattagcg ataatagcat gaggtttatc ctcaattgct gtgtttttta gtataaaaaa
      181 aaggaacaaa actgagacac ataaggcctc gcaatggctt gcaaggtttt acatattttg
      241 aggtagtggg acgtgtgaac gcagagatgg cgcggtaatt tgttgactta aaatgtcgtt
      301 cctggagttt ctaagtcgtg ggctacaggt tcgaatcctg cagggcgagc catttcctcg
      361 ccatttattt gttcccttat acaaattaca ccatcacttt gttgtcgcag ttttgctaaa
      421 taattggaac acgtcagacg aataaaatga aaacagaaat tctgctaaac aattcatctt
      481 gccaggattt tgattaggaa agtgaaaagg aaaggagcaa gctaagttgc tccctttaat
      541 cattaatact caaggatact ctgcgcgacg ggcaaatagc gcagagagaa atagagcgtg
      601 tctttaccgc aagaggaacg tttcgctaac agcgaggcga tctctgcatc cggtttcggt
      661 ttcgctgtaa acgttttacc tttaccagcg acaaacagtt cataaacaat gccgcttgct
      721 ggggcggaac tttggcaatc ggcgctggcc tgtagataac gttcacgctg ttcagcggtc
      781 agtttatcca cgccgctgcc atcctgtacc cagacattaa caccggttgc tttaacttgt
      841 tccagcagtt ggcgatagcc atctggcgac atgtttccgg cgaaaaaact actgatataa
      901 accggttttg ctgaaacatc gctaatcaac cgctgcgcgt tgtttaacca tgttagcaaa
      961 ggctgacggg cggcttcgct gcgccagttc aggtcgtcaa tttccgcgct gatgtaccag
     1021 ccatccggcg ttacgccagg cacggcgctc cataatctgg cttgctggag atcggcagcc
     1081 agcaggcgat taagatagct ttccagcgct gcggaggact gtttctggtg tataaaaaat
     1141 tccggatcgg cgttcagccc gacaatcagc ttcaggccag cctgttgcgc agctgtagca
     1201 cgcttaaaca ataacgcgcg ctgttctggc tgggtgaatg catcgccgta acgggtccat
     1261 tgcaaaacta gggtatcgaa gccttgcaaa cgtaactgac tcatcagccc ctgccactgg
     1321 gtatcggtaa cctgactatc acggttctgt ggttgccaga taataccttt catcgcaaag
     1381 gaaaaagggc tgaccaaaag cagtgtcagc aatacgaaaa tgaacttacg catttaccag
     1441 tgcactccaa tggtgagaaa cgcgttgttg cgctctccgt tacgttgatt aatcgcctta
     1501 aaggtatgtt gatattcgac gccgagactg actttgtgcg gccaggcgtc gtagtgcgtc
     1561 tcgccggtcc agatattcca gcggaccccg acaccgccaa gctgcgcgcc ctgagtgcct
     1621 ttatcacgat aaccgttgtc ctgaacgtga gcgtaaggct caatagtctg tccgttcgcc
     1681 accttctgat gccagcttac gcgataatct gccgtccacg cctgaatatc ctggcggata
     1741 tattgcgccg catcgaggta caggttttgg gcaaaccagc ctgaaccgtt cgggtgccat
     1801 tcgtcgctgt atttgccgcc attaaagaat gaggcgctgg cgcgcagcat ggtatcggat
     1861 gcgccatttt gcccgtttag cggcaactgc tgttcgacgg cgaggaaaaa gatctgatcg
     1921 cgcagcggct tccagcgcag accggtgccg gacatcggat ttttcaccgg catcatcacc
     1981 ccgttttcac cggtatcggc aaagacgcgg ctataaactg agagcaggtc gccttccagc
     2041 agcatattgc gtccgaggcg gtactcggct tccagttgcc cgtagctacg atagcttttc
     2101 cctggcgctg cgccgccgac attattgtta gcggtactca ttgcgccgga acgcaagccg
     2161 atagaagaat cgaaactgaa cgtccagcgg cgaccgacct cctcatgcag acggcggaaa
     2221 ttgaagcgtt gctgattttg ctctggggtc agtggggtta tcagcgcctg attatcaatg
     2281 tcatcaatca ccagtcgggc gtagtgctgc gtcgcaggca tgtcatccag acgctggttc
     2341 acgtaggcca gttgtcggat aagtgccgga tcgtccggta gccctttatg cgcctgttcg
     2401 agcatttccc gcgactgtgc gatatcaccg ctatcccaca aggcgtaacc gagcgctgct
     2461 tgggtgttgc tattattcgg ttccagttcc agcgcggcgc gcaaatcact caccgccgcc
     2521 gggacattat gacgttggcg ataaattgtc gcccgcgcaa cgtaagcgtt ggcagaaggc
     2581 gcaatattga ttgagcgcgt gagatcgttc agtgcaagtt ccggctgacc aggaatgtaa
     2641 cgttgcgcat gcagccacca gtagagggca ttgtttccca gcccacgttg ttctgcctgc
     2701 tgaagccagc gatcgcgagc cgcaccattt cctgccgcct gggcggtatt ggcagcagca
     2761 agcagatcct cattgctcat gtcgtgaaga ctgattttct gccaggccgc cagtgcggtg
     2821 gcgtagtcct caacctgata cgcctgatag gctaccgcac gatgttgcca ggcgtccggt
     2881 tgtcgttgtt cggcctgaag ccatgcatac aacgccacac cgggtagcgt gtcccgataa
     2941 cactttgcca gacggttcca ggcggcggca tcgtaggaag gcgacatatc gcccagcaag
     3001 cgaactattg ccgggcaatt atctgcaata cccggcaact gactttgcca ctgacgttgc
     3061 tccgccagcg gtaagggttt cgataaaatc gccaccttcg ccggcgttgc caggtaagga
     3121 tgactttcca gcagagacgc cagtcgcgcc attaaagtct ggctgacacg cgcatcgccc
     3181 tggaaaggat agcgttgcag caataaatcg gcagcttcgc gtgactgttc gttctgcatc
     3241 agttgccagg tcagttgatc caggcgggta agatttgccg gttcttgctg atacagcaat
     3301 cgtgccagac gcagagcttc agccttgtta tgggtcgcta cgctaacggt ataacgctcc
     3361 tcaagcattt cattggcggg gagggtggcg agcagttttt gcgctgcgcc gtactgacct
     3421 tcttttaaca gcaccggtag cgtcgcgcca acaacatact ggcggttgtc ggcaaactgt
     3481 accgtataat tcgccaacgc ctgaacgggg ttgccgctgt atttagataa cagatagagc
     3541 caacttttct cttgtgcgtc cgtggtaaat agcggcgtat tttcaatgag ataatgctgg
     3601 aggcgtgctt tttcgccacg ataagccagc gcggtcgcgt aagtaatata tgactgagga
     3661 tcggtgaaga tcccctgtga ttgcagcgcc aggatccgat cgtccaactg cccggcaaga
     3721 agcacgtcaa accactgacg gcgttctgcc gcgcttaatg tgttctgctg gcgtgcttca
     3781 ttgtatagcg tatctgcctg ggaccattgt ttcaggtaga ttgcgcgttg cagcagatcg
     3841 gttcgcagcg tttttccttc cggcgatgca gcaaacgtcg catcgttcag ttgcgctctg
     3901 gcgacaggta attgtgccag ccgcagggca ttctgcccga cttcactgcg acaacgcagg
     3961 gtcggcgcag catcgcacgc tttttgctgg gcaagcagtt cttcaacagt cgtaacgctt
     4021 ttcacttcaa caggaatagc cgccagactg cgctcaagcc gatcatctcc agggtgacgt
     4081 ttcagttgat cctcaagcaa cagccgcgcc cggtcatcat gaccaaaatg gcgataggct
     4141 tccgcaaggt ataaagtcag cggaatatta tccggcacct gctggtgtat atattcaaat
     4201 tcgcggatgg cggttgcttc gtcgttattt ttctgtgcct tcagcgcctt atcgagacgg
     4261 ggataaataa caaaatggcg ataatcgctc agccccagct cttctgcgct ggtgccgata
     4321 ttgtctgcga gtgcgctggt actcaataaa gatgtcagca gtaaaccaga ccatccgatg
     4381 acgcgattaa ggttattctc cttcattttc ggactccagt tgtgcaacct gttcagtgtt
     4441 taaacctgct ttgagtaata gtgattgcat cgaaacttgt aattcgcgtt gaattgtcag
     4501 gacgcgatcc aacgtttcct ggctgataac gccttcggtg accaaaaact tgccgagcgg
     4561 cagagaactg cgttcatggc gcaataacaa cacgttaatt gctgaacgat taatatgacc
     4621 gagcgtggtc agtatttctg cgaacaggaa ctgatgcggc acatattgcc gccagatttc
     4681 accggcctgc tgttccgtga gccactgatg ctgaacagca ttgtacaaca ttgcccgcgg
     4741 atcgtgaccg cgtcggcgtg cataccagtg gcgtaatccg gtgacaattt gtccccgcag
     4801 aacaatgacg taacgcactt tgcgtccgac tttacgcgtc agggccgcca gcgaaaccgg
     4861 gtcaatacca tcttcactgc cgacaattaa ctcatcattg tccagacgca gcggcagtac
     4921 cgcataatgc aacgccacgg aggccggcat ttcggcaatc agcgaggaag ggatctgcca
     4981 ggcatcgatg gattcccacg ccacgccgtt ttgctctgcc agcgcctgtg ccagttgctc
     5041 ggcgctaatc agcccctgca tcagcattga accgcccagg cgtagacctt cgacgcgatt
     5101 acgcagtgct gtatcgagtt gttcttcagt gatgacctga ttttccagca ggatttgacc
     5161 taacggacgc aacgagcggg tatcgccagt cacgctgggg aagtcatgcg ttgttttatc
     5221 ccacgcgaca cgacgtggat cgccgtgttg aagtacctgt tttaacgcgc gccagttggc
     5281 catgaagtta atcaggttgc cccagaaaag acgcaggaca gatagcagcc cctgcgtcag
     5341 gccgtagtag ccagtaacga aaatcacccg ctgcacgatg cggttaatca tcaagccaaa
     5401 gtttagccac agcagagtca ttaaccatgc gctgccgctg aaaatagaaa ggaaatgcca
     5461 ggcatcgggc cacaaacttt catacgccag caacagcaaa agctggatca tcaccagcat
     5521 cgcgaggaag ctgacaaagt tactgattgc ccctttgcgg tcgcgccaga gaaagtagtt
     5581 cagcgtcagg ctggaggtcc atttgtgggt tttaaagccc tggaaaacaa tgccgatgat
     5641 ccagcgagat ttttgccgaa ccgcagtcga aaaggtatcg gggaaatatt cgcgcacgca
     5701 gatcatgttt gacgtccgcg catgctgtaa aaatttacgc tgctcgcgtt ctttggcttc
     5761 gtccaccacc ggaaaacgga caaaaatttc cgtcatacct ttctctttca ggcggaagcc
     5821 aatgtcgtaa tcttcagtca gactctgcac gtcgaaagca ataccgtcac cgtcagctaa
     5881 cagtgcggtc acggcgcggc ggctgaaaca ggtaccgacg cctgcgctgg gcacctgtcc
     5941 ggcgagggct tcacgcaccg gaacatcttt gccatgcagt tctgaaaact catcaatgta
     6001 ggtcatgctg gtgaagtgcg tccattcgcg ttcgaacgga tacactggga tctgaatcag
     6061 atctttacgc tcgaccagat agttgaacag acgcaattcc atcggtgaaa tcacatcttc
     6121 ggcgtcatgc agaataaaac cagcaaaagc gaaattggcg ctacgttcaa actgggtgat
     6181 ggcgtccagc acgttgttca gacagtcggc tttgctggtg gggccaggac gcgcgcagac
     6241 taccttatgc acgttcggga agcgagcgca cactttgtca acatcacgct gagtatcggg
     6301 gtcgttgggg taggtgccaa caaagatatg atagttttcg tagtcgagcg tggtcgccgc
     6361 cagctcggcc atattgccga tgacgcccgt ttcattccac gccggaacca taatcgctaa
     6421 cggtttttca tctggtttat acagttcgcg gtaactcatt cgcgggtagc ggcgataaac
     6481 actcaacttg cgtttaatgc ggcgtaccca gtagacgaca tcaataaaaa aatcgtccag
     6541 cccgctgatg aacataatga ccgctaacgt tatcgcgatt acttttaagc cgtagagcca
     6601 ggtagcaaaa acatcaagaa gccagtccac acaaaaacct tacattaacg ctggttatgt
     6661 ttagggtggc gtatattaag gttttttatg aattgtgaca gctttttatc attaataggt
     6721 attactattg cggcatgtta tttttacact ggttataaaa gttgccgttt gctgaaggat
     6781 taagcgggta atatgataac aaaccttgtc ccccgcacat ctgacgttac tgcaactgtg
     6841 cctttatgag cgacaacaat cgatttcact atcgccaggc caatgccgct accttcgcct
     6901 tttcgctggc gggaaggatc aacgcggtag aaacggtcaa acaatcgcgg taagtgttcg
     6961 ggcgcaatgg gcgtaccggg gttttcgacg gtaacttgca ccaggtgatc gaccgtctgg
     7021 cagcgcacga caattgtctc tccggttggc gtataacgca gggcattaga aagcagattg
     7081 cttaacgccc gacgcagcat cagcggatcg cccgcaacct gacactcgtc gccaacaaac
     7141 cgcagctcca cgccgcgatc ttctgctaac gcctcgaaaa aatcgaacac tttgccgact
     7201 tcatccgcca ggttgagcat tttcttttcg gggattagct ggttgttatc ggcctgagcg
     7261 agaaacagca tatcgctgac cattttcgcc attcgcgtca gctcttcgag attagagtag
     7321 agcacatctt ccagctcctt ctggctgcgc gactggctga gggcgatttc cgtttgcgta
     7381 atgagattag taataggtgt gcgaatttcg tgggcgatat ccgctgagaa attggactgg
     7441 cgggtaaaga catcctcaat acgctcgatc atatggttga acgacagtac cagctgttcc
     7501 agctcaatgg gcacggtctg cgggtcaagg cgaacgtcga gatctttcga ggtgatattc
     7561 tggatttgac ggctgacgct gcggatcggc gcgtgacctt tatgtaccgc caacagcacg
     7621 ataaagacga tcaggatgct gattaccgat gcggtcataa taagtttatt catcaaatca
     7681 ttgatgtaat gaagatgaaa atcgatcgaa agcgcaatgt agagcgtata aatcggtttg
     7741 ccgtcaacca gcgggccaac cggcaagtta atcatccgcc agttgctgtg ttccatatgc
     7801 ccgtgaccgt gccctggcat catcatcgtt gggccggaaa ggagatacac ctcgccgccc
     7861 tgcgcgtctt tatcgggtat ggcgtcacgc gtaaactcgc ggatatccgg cgcaccgggg
     7921 gagtgataca ccgttttacc gtggctatct gccagagaga tcaacacatt ggaataacca
     7981 ctgacgatat cttccagcgt cattaagcgt cgggcttgcg tttcgtcagg gtgattaagt
     8041 acccgttcaa gggtggcgct aatctctttt aaatcattaa tatcctgctc ggcaaaatgc
     8101 acttttaccg agtggatcat gatccatgca aaggcgaaaa acgccgcgat ggtggcgagg
     8161 ctgataaaaa aggtcaggcg agttgccagc gaaaacgggc gctgaaatgg tttactgacc
     8221 atccggcacc tctagcatgt aacccacgcc gcgcacggtc tgaatcaact ttggctcaaa
     8281 gtcgttgtcg attttgccgc gcagccgctt caccgccaca tcaatggcgt tggtatcgct
     8341 gtcaaaattc atgtcccata cctgcgaggc gataagcgag cggggcagca cttcgccctg
     8401 atggcggagg aaaaactcca gcagagtaaa ttctttactg gtcagagtga tgcgcgtgcc
     8461 gctgcgggtg actttgcggc tgacgagatc gaccatcaga tcggcaacct gaaactgact
     8521 ttcgataatc accgccgccc cgcgccgcag tagagtgcgc acccgcgcca gcagttcagc
     8581 aaaagcgaac ggcttcacca gatagtcatc cgcccccaac tccagcccct tgacgcgatg
     8641 ttcaatggtg ccaagcgcgg taagcaacag aatcggcatc cccttattgg cggaacgcaa
     8701 catgcgcacg atatcccagc cgttcacgtc cggcagcata atatcgagga ttatcagatc
     8761 ataatcaccg gtcatcgcca gatggtagcc attcagcccg ttgtcggcca aatcgaccac
     8821 aaaaccggct tcggttaacc ctttggtcaa gtattctccg gttttctttt catcttcgac
     8881 aatcaacagt ttcatatttc ctccgcatgt tgcccgggca attctagagt agcgggatca
     8941 gatggcaatc gcttattggc aaaatgacaa ttttgtcatt ttcctgtcac cggaaaatca
     9001 gagcctggcg agtaaagttg gcagcataaa atcaccagaa attatgagcc tatgtctcct
     9061 tgtaaacttc tgccattttg tgtggccctt gcgctaaccg gttgttcact ggcaccggat
     9121 tatcagcgtc cggcaatgcc cgtgccgcag cagttctcac tcagccagaa cggcctggtt
     9181 aacgcagcag ataactacca gaacgcgggc tggcgcacct tttttgttga taatcaggtg
     9241 aagacgctga ttagcgaggc gttggtgaat aaccgggatt tgcgcatggc ggcgctgaaa
     9301 gtgcaggaag cgcgggcgca atatcgtctg accgatgccg accgctaccc acagctcaat
     9361 ggcgagggca gcggcagctg gagcggcaat cttaaaggcg attcagccac gacgcgggag
     9421 ttctcgactg gccttaacgc cagctttgat ctcgattttt tcgggcgctt aaagaacatg
     9481 agcgaagccg agcgccaaaa ttatttagcc actgaggaag ctcagcgcgc ggtgcatatt
     9541 ctgctggttt ctaatgtcgc acaaagctat ttcaatcagc agctggcgta tgcgcaattg
     9601 caaatagccg aagaaacgct gcgtaattat cagcagtcat atgcgtttgt cgaaaaacaa
     9661 ctgttgaccg gtagcagcaa tgttctggcg ctggaacagg ctcgcggggt gattgaaagt
     9721 acccgcagcg acatcgctaa acgtcagggg gaactggctc aggcgaataa tgcattgcaa
     9781 ctgttattgg gaagctacgg caagctgccg caagcgcaga cagtaaacag cgacagcctg
     9841 caaagcgtta aattaccggc gggcttgtcg tcgcaaatct tattgcagcg cccggatatt
     9901 atggaagctg aacacgcgtt aatggcggct aatgccaata ttggggccgc acgcgcggca
     9961 tttttcccgt ctataagcct gaccagcgga atatcaaccg ccagcagcga tctgtcgtca
    10021 ttatttaacg ccagcagcgg gatgtggaat tttataccca aaattgagat ccccattttt
    10081 aatgccggac gtaaccaggc caatctggat atcgccgaaa ttcgccagca gcagtcggtg
    10141 gtgaattatg aacagaaaat ccagaacgcc tttaaagaag tggcagatgc gctggcatta
    10201 cgtcaaagcc tgaacgatca aattagcgcc cagcagcgtt atctggcgtc gctgcaaatt
    10261 actttgcaac gggcgcgggc attatatcag cacggcgcgg taagttatct ggaagtgctg
    10321 gatgctgagc gttctttatt tgcaacccga caaactttac ttgacctgaa ttatgcccgt
    10381 caggttaatg aaatttcttt atataccgcc ttagggggcg gttggcagca ataattttta
    10441 actccaggag agaataaatg aaaaaagcac tgcaagtcgc aatgtttagt ctgtttaccg
    10501 ttattggctt taatgcccag gctaacgaac atcatcatga aaccatgagc gaagcacaac
    10561 cacaggttat tagcgccact ggcgtggtaa aaggtgttga tctggaaggc aaaaaaatca
    10621 ccatccatca cgatccgatt gctgccgtga actggccgga gatgactatg cgctttacca
    10681 tcaccccgca gacgaaaatg agcgaaatta aaaccggcga taaagtagcg tttaattttg
    10741 tccagcaggg caatctttct ttattacagg atattaaagt cagccagtaa tccgggttta
    10801 atgagatgaa aaaaatcgcg cttattatcg gcagcatgat cgcgggcggt attatttctg
    10861 cggcaggttt tacctggttt gcaaaggagg aaccgcccgc agaaaaaacg tcgaccgcag
    10921 aacgtaaagt cttattctgg tacgacccga tgtatcccaa tacgcggttc gataaaccag
    10981 ggaaatcgcc gtttatggat atggatctgg tgccgaaata tgccgatgaa gagagttctg
    11041 cgtctggtgt gcgcattgac ccgactcaga cgcagaatct gggggtgaaa acggcgaccg
    11101 tcacgcgcgg gccgctgact ttttcccaga gtttcccggc gaatgtcagt tacaacgagt
    11161 atcagtatgc cattgtgcag gcccgcgccg ccgggtttat cgacaaggtg tatccgctta
    11221 ccgtgggcga taaagtgcaa aagggcgcgc cgcttctcga cctgaccatt cccgactggg
    11281 tggaagcgca gagtgagtat ttactgctgc gcgaaaccgg cggtacggcg acccagactg
    11341 aaggcattct tgagcgactg cgactggcgg gaatgccgga ggctgatatt cgccgcctga
    11401 tcgccacgca aaaaatccag actcgcttta cgctcaaagc gcccattgat ggcgtgatca
    11461 ccgcgtttga tctgcgcgcg ggaatgaata tcgccaaaga taacgtggtc gcgaaaattc
    11521 agggtatgga cccggtgtgg gtcactgctg cgatcccgga gtccatcgcc tggctggtga
    11581 aagatgcctc gcagtttacg ctcaccgttc cggcgcgacc tgataaaaca ctcaccatcc
    11641 gcaaatggac gctgctacct ggcgtggatg ctgcgacccg cacgctgcaa ctgcgtctgg
    11701 aagtcgacaa cgccgacgag gcgctaaaac cgggcatgaa cgcctggttg caactcaata
    11761 ccgccagcga accgatgctg ctcattccgt cacaggcgct gattgatacc ggcaacgaac
    11821 agcgggtgat taccgttgat gctgacgggc gctttgtacc gaaatgcgtt gccgttttcc
    11881 aggcatcgca aggcgtcacc gcgttacgct ctggtctggc ggaaggtgaa aaggtggttt
    11941 ccagcggcct gttcctgatt gattctgaag ccaatatttc tggcgcactg gagcggatgc
    12001 gctctgaaag tgctacccat gcgcattgag ggaatagcca atgattgaat ggattattcg
    12061 tcgctcggtg gcgaaccgtt ttctggtgct gatgggcgcg ttgtttctga gtatctgggg
    12121 cacctggacc atcattaaca cgccagtgga tgcactgccg gatctttccg atgtgcaggt
    12181 gattattaaa accagctatc ccggtcaggc accgcaaatc gttgaaaatc aggtgactta
    12241 tccgctaacc accaccatgt tgtcggtgcc tggcgcgaag actgtgcgcg gtttctcgca
    12301 gtttggcgac tcttatgtgt atgtcatttt cgaagatggc accgatccgt actgggcgcg
    12361 ctcgcgggtg ctggagtacc tcaaccaggt acagggtaag ctgcctgcgg gagtcagcgc
    12421 cgagctgggg ccagatgcca cgggtgttgg ctggatctat gaatatgcac tggtggatcg
    12481 cagcggtaag cacgatctgg ccgatttacg ctcattgcag gactggtttc tcaaatatga
    12541 gctaaaaacc atccctgacg ttgcggaagt ggcgtcggtg ggcggtgtgg tgaaagagta
    12601 tcaggtggtt atcgatcctc agcgcctggc gcagtatggc atcagtctcg ccgaagtaaa
    12661 aagcgcgctg gatgcttcaa accaggaagc gggcggttcg tcgatcgaac tggcggaagc
    12721 ggaatatatg gtgcgcgcca gcggctatct gcaaacgctc gacgacttta atcacatcgt
    12781 tttaaaagcc agtgaaaatg gcgtgcccgt ttatctgcgt gatgttgcga aggtccaggt
    12841 tggcccggag atgcgccggg gcattgccga actgaacggc gaaggcgaag tggcgggcgg
    12901 ggtggtgatc ctgcgttccg gcaaaaacgc ccgtgaagtg atcgccgccg tgaaggacaa
    12961 actggaaacg ctgaaaagca gtctgccgga aggcgtggag atagttacaa catacgatcg
    13021 cagtcagctc attgaccgcg ccattgacaa cctcagcggc aagttgctgg aagagtttat
    13081 tgtggtggcg gtagtctgtg cgctgtttct ctggcatgtg cgctcggcgc tggtggcgat
    13141 tatttcgttg ccgctggggt tgtgtattgc ttttattgtc atgcactttc agggactgaa
    13201 cgccaatatt atgtcgttgg gtggcattgc gattgccgtc ggggcgatgg tcgatgccgc
    13261 tatcgtcatg atcgagaatg cgcataaacg gctggaagag tggcagcacc agcatcctga
    13321 cgccacgctg gataataaaa cgcgctggca ggtgatcacc gatgcatctg ttgaagtggg
    13381 gccggcgctg tttatcagtc tgctgattat cacgctgtcg tttatcccga ttttcaccct
    13441 ggaagggcag gaggggcgtc tgtttggccc gctggcgttc accaaaacct atgcgatggc
    13501 gggcgcggcg ctactggcga tcgtagtgat cccgatcctg atgggctact ggatccgtgg
    13561 caaaattccg ccggaaagca gtaacccgct caatcgcttt ttgattcgtg tttatcatcc
    13621 gctgttgctg aaagtactgc actggccgaa aaccacgctg ttggtggcag cgctttcggt
    13681 gctgacggtt ctctggccgc tcaataaagt tggcggggaa tttttaccgc agatcaatga
    13741 aggcgacttg ttgtatatgc catcgacgct gccggggatt tccgcagcgg aggcggcgag
    13801 tatgctgcaa aaaaccgaca agctgattat gagcgtacct gaagtggcgc gggtatttgg
    13861 caaaaccggg aaagcggaaa ccgccaccga ttctgctccg ctggaaatgg tagaaacgac
    13921 tatccagctt aagccgcagg atcagtggcg gcctggcatg acgatggaca aaatcattga
    13981 ggaactggat aacaccgtgc gtctgccggg gctggcgaat ctgtgggtgc cgccaattcg
    14041 taaccgtatc gatatgctct cgactggcat taaaagccct attggcatta aagtttccgg
    14101 cactgtgctg gcggatatcg acacgatggc ggagcaaatt gaagaagtgg cgcgaacggt
    14161 gccaggcgtg gcttctgcgc ttgctgagcg tctggaaggt gggcgctata tcaacgttga
    14221 gattaaccgt gaaaaagccg cgcgttacgg tatgacggtg gcggatgtgc agttgtttgt
    14281 gacttctgcg gtgggcgggg cgatggttgg cgaaacggtg gaagggattg ctcgttatcc
    14341 aattaatctg cgttatccgc aaagctggcg cgatagcccg caggcactgc gccagttgcc
    14401 gatcctcacg ccgatgaagc agcaaatcac cctggcggac gtggccgacg ttaaagtctc
    14461 taccggaccg tcgatgctga aaaccgagaa tgcgcgcccg acgagctgga tttatatcga
    14521 tgcccgcgat cgtgacatgg tgtcggtggt tcacgatctg caaaaagcga tagctgaaaa
    14581 agtgcagtta aaaccgggca ccagcgtggc attctccggg cagttcgagc tactggagcg
    14641 cgccaaccat aagctgaaac tgatggtgcc gatgacgctg atgatcatct tcgtgctgtt
    14701 gtatctggcg ttccgtcggg tgagcgaagc gttgctgatt atcagcagcg taccgtttgc
    14761 gctggtgggc ggcatctggt tgctgtggtg gatgggcttt catctttccg tggcgacggg
    14821 caccggtttt attgcccttg ccggggtcgc cgccgaattt ggcgtggtga tgctgatgta
    14881 tttacgtcac gccatagagg ccgagccgtc gttgaataat ccgcaaacat tcagcgagca
    14941 gaagctggat gaggcgttat atcacggcgc ggtactgcgc gtgcgcccaa aagcgatgac
    15001 ggtggcggtg attatcgctg gtctgctgcc gattctgtgg ggaacaggag cagggtcaga
    15061 ggtgatgagc cggattgccg cgccgatgat tggcggcatg atcaccgcac ctttgctgtc
    15121 gctgtttatt atcccggcgg cgtataagct gatgtggctg caccgacatc gggtacggaa
    15181 ataaaagcag gataccccgt ttaaccgtgt ggattgtgtc ttgcgacgat gggcactaaa
    15241 tgttaaaagg tgcccctcaa caaaaaagac acacagggga aaggcgtgaa aaacgcgtca
    15301 accgtatcgg aagatactgc gtcgaatcaa gagccgacgc ttcatcgcgg attacataac
    15361 cgtcatattc aactgattgc gctgggtggc gcaattggta ctggtctgtt tcttggcatt
    15421 ggcccggcga ttcagatggc gggtccggct gtattgctgg gctacggcgt cgccgggatc
    15481 attgctttcc tgattatgcg ccagctcggc gagatggtgg tcgaagagcc ggtatctggt
    15541 tcatttgccc actttgccta taaatactgg ggaccgtttg cgggcttcct ctctggctgg
    15601 aactactggg taatgttcgt gctggtggga atggcagagc tgaccgctgc gggcatctat
    15661 atgcagtact ggttcccgga tgttccaacg tggatttggg ctgccgcctt ctttattatc
    15721 atcaacgccg ttaacctggt gaacgtgcgc ttatatggcg aaaccgagtt ctggtttgcg
    15781 ctgattaaag tgctggcgat catcggtatg atcggctttg gcctgtggct gctgttttct
    15841 gggcacggcg gtgagaaagc cagtatcgat aacctctggc gctacggcgg tttctttgcc
    15901 accggctgga atggactgat tttgtcgctg gcggtaatta tgttctcctt cggcggtctg
    15961 gagctgattg ggattactgc ggctgaagcg cgcgatccgg aaaaaagcat ccctaaagca
    16021 gtgaatcagg tggtgtatcg catcctgctg ttttatatcg gttcactggt ggttttactg
    16081 gcgctctacc cgtgggtgga agtgaaatcc aacagtagcc cgtttgtgat gattttccat
    16141 aatctcgaca gcaacgtggt ggcttctacg ctgaacttcg tcattctggt ggcatcgctg
    16201 tcggtgtata acagcggggt ttactctaac agccgcatgc tgtttggcct ttctgtgcag
    16261 ggtaatgcgc caaagttttt gactcgcgtc agccgtcgcg gcgtgccgat taactcgctg
    16321 atgctttccg gagcgatcac ttcgctggtg gtgttaatca actatctgct gccgcaaaaa
    16381 gcgtttggtc tgctgatggc gctggtggta gcaacgctgc tgttgaactg gattatgatc
    16441 tgcctggcgc atctgcgttt tcgcgcggcg atgcgacgtc aggggcgtga aacacaattt
    16501 aaggcgctgc tctatccgtt cggcaactat ctttgcatcg ccttcctcgg catgattttg
    16561 ctgctgatgt gcacgatgga tgatatgcgc ttgtcagcga ttctgctgcc ggtgtggatt
    16621 gtgttcctgt ttgtggcgtt taaaacattg cgtaggaaag tgaattaagc tgatgtgagg
    16681 ctgatttgcc cgatgcgcaa gcttatcggg cctaaaattt gtaacgattc tgtaggtcgg
    16741 ataaggcgtt cacgccgcat ccgacaaaac gatgtcaacc atccgaaacc gctctcatcc
    16801 attcgatgag agcggttttt ttaattactg cttaaatgca cccgccagag agcgaatatc
    16861 attgccggtt ggcgactgat gaagtcgcag accaaactct tcgacaatcg caaatatgtg
    16921 atcgaatata tcagcctgaa tgctttcata ttccagccac accacggtgt tggtaaacgc
    16981 atagatctca agcggtaaac cgttgtcgcc cggagctaat tggcgcacca ttaaggtcat
    17041 atctttgcga atccgcggat gattccgcag atattcgttc agataggcac gaaaggttcc
    17101 aatattggtc attcggcgca ggtttaatac cgactccgta gaaccttgct ggcgattcca
    17161 ctcattaatt tcctgatggc ggctggttaa ataaggcttt aacaagtgcg ctttattcag
    17221 ccgttgcatt tcgtcttcat caagaaaacg aatactggtg acatcaatac tgatactgcg
    17281 cttaatacgt cgcccgccag atgctgacat cccgctccag tttttaaagg agtcagaaac
    17341 cagagaccag gtgggaatag tggtaatggt attgtcccag ttacgcactt tgacggtggt
    17401 taacccaata tcgatcaccg cgccatccgc gccgtatttc ggcatctcca gccagtcgcc
    17461 cagtttcagc atatcgttcg cggaaagctg aatacctgcc accagaccaa gaatcggatc
    17521 tttaaatacc aacatcagca cggcagccat tgcaccaaga ccgctgatca gaatcgctgg
    17581 cgactgacca atcagcagcg agatcatcaa aatgccgacc agaatcgcgc cgatcagttt
    17641 aatcccctga aatatccctt tcagcggtaa ctgagatgcc gccgggaatt tctgcgccag
    17701 attcaaaata acatccagca acgagaagac tgaaagcagc gcatacatca tgatccacaa
    17761 ctgcgcgcag gtagtcagaa tatctgccgc ttcggtgcct ttttgtagcc agaataccgc
    17821 ctgaatattg acgataatcc cctgcagggt aaaagctaaa cgatggaaga gtttattctg
    17881 ggtaatgatt tgcaaccaaa gccgtgaact ggcaatggcg cgtttttcga aggtccgcag
    17941 taccacccaa tgcaaaataa tatgcaccac gacggcggtc agaaaaataa taccgaaaat
    18001 catcaccatc gaggtggtgt gatcaatctc aatacccgct aaatcttcaa cctgggatat
    18061 taaatcctgc ataacgtctc ctttatacaa cagcagccta tgatgacggc tgaaacaggg
    18121 ttatgcaaat caggagaatc tgaggggaaa tagccgggca gatgcccggc aagagagaat
    18181 tacacttcgg ttaaggtgat gttttgcggc agacgagatt tcggcagcgt agcgttaaaa
    18241 tcttcaacgc tgtgatgacc caccggaaca accaccagac tggtgtagcc tttctctttc
    18301 agaccaaatt cttcatcgag gatggcggcg tcaaaacctt cgatgggcac cgcgtccaga
    18361 cccagcgccg ccacgcccag caggaagtta ccgacgttga gataaacctg ttttgccatc
    18421 cactctgcat catcatgcag atctttacgg tgcatatcgg cgaagaactt gcgaccttta
    18481 tcgttcgcgg ctttcgcttc cggcgtggca aagcggccat cggcatcttc ctggtcaaca
    18541 accagcttca gccaggcatc gtccattgcg gtttttgcac agaacaccac gacgtgcgag
    18601 gcatcaagca ttttacgctc gttgaacacg taattatcgg cagcggattt ggcaacacgc
    18661 gctttacctt cttccgtgct ggcaacaata aaatgccacg gctgggagtt ggtgctggat
    18721 gggctgtatt gcagtagagt tttgatctgc tcggcctgtt ccggggtaag ttttttgctg
    18781 gcatcaaatg ccttagtgga atgacgcttt aaggcgacag aaatgatatc cataaagact
    18841 ccatgtgaaa gtaattttgc gtgccagcag attacaagga tcgagatgaa atggtaagcg
    18901 agaaaaatgc gctattgatt tacggttgag tcaggacgca gtcgtttttg atcgcgttta
    18961 gccagaccat caaccaccag attccacgaa tcgttgatca gatcgcgaag taacgcttcg
    19021 gagatttcct cgccgggata caccgaaatc cagtgctttt tattcatgtg ataccctggc
    19081 ttgatgcttg ggtatatttg ctgatttagc agggattttt gtggatcgga cttcagattg
    19141 ataaagggga cgccgcgcag ctccgacgac agcataaaaa tcttgccacc aattttaaaa
    19201 acatcgaact ccgggccaaa aggccagcaa agctcgacaa agggtaactc aagagccagg
    19261 cgtttcgccg tttcgtgcag tgattgctta tccataaatg ttcctttagg cgaaggagaa
    19321 taagcaaagt atgccgcgaa gttcggcgat aatcgacgtt taatccgcca gcgagaacca
    19381 gcgtcgccag ataaagcgta gaacaaaata ctcaatagcg cccagcacta aaaaccacag
    19441 acaaaacaat aacgtgtaaa gctggctaag atccatcaga tggaacgtgg tcaccagttt
    19501 ttgtgccagc gttagcccca gtgcgggggc gggcagcagc aggcaagaga ggaaagccat
    19561 cagcaaaatg cctgctgcgg tggtcaaggt ttctaaaggg tgtttcataa cattgttaaa
    19621 tgtaagttaa aacaccattg tcagggatat tcttctgtaa ggcaattccc gacttattct
    19681 ccgacccaga tctcacaatg ctttttcacc agcccaatta gcgagccacc atcggcgacg
    19741 aaatcacgca ttagctgcgc ttcattcaga ccactgacga cctgacggtg cagtgcttca
    19801 attgcgctcg atgcgccaat tttatgtgca gaaggggcga ttttttccag taaacgcaag
    19861 gtggcttccg ttagtgaccg acgatcgcca gtatgcggat cggtaatgac gccttccagc
    19921 ccatagcgac aggcctggaa acggttgaat ttatacagca ggtaatcttt ctcctgatgt
    19981 ttaaacgggc gttccgtcag taaccagtgg gcggtggcct gaattaatcc cgccatattt
    20041 accgcgtggc taagggttaa cggggtatcc atcacccgaa cttccaccgt accaaaatgg
    20101 gggctggggc gaatatccca gtgcagatct ttaatgctgt cgatcatcgt ggtgtaactc
    20161 aggcagcgaa acagggcttc aaattgttgc cagttactga cccacggcat cgggccatta
    20221 tcaggaaagg cggaaaaaat attcggtcgt gaggaggcaa aacgcgtatc cgttccttgc
    20281 atatatggcg acgcggcgga aagggcgata aagtgcggca caaaccgtga caagccgtgc
    20341 agcaaataaa tggcgtcatc gccactggcg cagccaacat ggacatgctg accaaaaacg
    20401 gtcgcctgct ggatgagata gccaaagttt tccagcgttc gttgatagcg ttcgttatcg
    20461 catacctcct gacgctgcca tttctgaaac ggatgcgtgc caccgccgca aatttccaga
    20521 tgatggtctg cggctgcctg caatacgact ttctgcatcg ctgaaaattg cccggcagcc
    20581 tggttgatat cacggcaaac atccgtcgcc agctccagca tactttcggt gatatcgtgc
    20641 tttacctctc cggccgtgat ctgattttta accgtgtcaa tcagcattga tgagtcctgg
    20701 cttaagtcat agcccggcgg attaaccacc tgcatttcca gttcaatacc gagggtaaaa
    20761 ggttcagaaa catgaaaatc gggtaatggc ataggtttct cttatgttgg cgttttctat
    20821 tcagtataga agtgggagcg gctgggtgag atgcggaagt tctggaatgt ttcttttttt
    20881 ggtgatggag aactgattgt gactgaagca atttggctac ttttgcaatg tgacaagtta
    20941 tggcagagct ggctggcggc gaagaatttt gacgattgag gcatgcagaa aaaaacgggt
    21001 tcagcttcag ttgattaaat gagaagtccg aaacaggact cactgtataa ataaacagct
    21061 attttgctaa ggaagggtaa gataactgcg ggtgcctgaa gctttccggt ttcaggttta
    21121 ctctgaggtc tggaaagatg aagccccagg acgatatttc tatcaaccct ggggctacct
    21181 acccaaacct gaacaatttg gatggtagtc ccttcttcgc atggaggcaa tataaacatg
    21241 ctgacgaaat atgcccttgt ggcagtcata gtgctgtgtt taacggtgct gggatttacg
    21301 cttctggtcg gagactcgct gtgtgagttt accgtaaagg aacgtaatat tgagtttaag
    21361 gctgttctcg cttacgaacc gaagaagtag ccgttgtgcg gggagcaatc cccgcatatc
    21421 cggttgtcag gtcagggtgg taaggcacct gctttacact ttcgcccgcg gtcagtgatg
    21481 gctgcgggcg aatcgtacca gatgttgtca attaatcgtg ttggcacagc gttatgacta
    21541 ttttttcttt tatctgccag tgcacagcaa actgctcatc ctctagtcga atgatgatct
    21601 gctgtttatt ccagctaatt atctgataat ccagaaaacc ttgagcggcc tgtatctcac
    21661 ttgccttaaa tgcgctctct ttggcggaaa atgccagtgt cagcgccaga ggaaaggcta
    21721 aaccgcagtc tgcaagtcgc ttgtgttccg ctggtgtaat aatgttgtct gtcaattctc
    21781 ttgcggtttg tgcagagaaa atctcttcaa tatcaatgcc aattggttga cgggaaacca
    21841 cggctaatgc cgtagtccca cagtgactaa tgctgccgta tacccccgca ggccacacag
    21901 gttggcgtag ctcgccgatt gcaggcacac atttatagcc atattcccgc agcgcataaa
    21961 ttgcagcgat ccgcccggct aaatgctctg ttttacgttt acgtccagcg tgttgaagtt
    22021 gtgcgtagtg cggcagccag agtaaatcct gctcgcgaaa actcgccgga tcaaactcaa
    22081 caaaatgcag cgtatgtccg gcaaagggga gggaggtatg cgtagttttc atgtcaacca
    22141 tattagagaa tgatgacaaa cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt
    22201 ggtcctgcaa tatattgaat tggcaagatt tttgtaggcc ggataaggcg ttcacgccgc
    22261 atccggcatg aactacgcgc actttgtcaa caatctgacg ttagcttcag aagtgagtat
    22321 taatgctcat ataccacgta cgtcccggct cgttataggt atacgccccg gcacctgcga
    22381 tatagttggc tcctgccaga tcgcccgtgg tctgggcatt acccgcacgc cacaaacgtt
    22441 tgtcgaacag gttgtccacg ccgccggtca gactgacatt cttggtcaca tcccaggtcg
    22501 cgctcaggcc aacaatgctg tacggactga tttctttggt ttccggtcca accgctggct
    22561 gacctttata gttgtacttc ttcggctgct gtttgccgta ccaggtgaag gtcgtttgca
    22621 tcgacaaatc ttcccgtgcc tgccagctca gcgttgagtt caacgtatat tccgggatga
    22681 tcgacaaacg gtcgcccgtg gttttgtttt cactcttcag catataagtg atgttattgg
    22741 tccacatcac cgtttcgcta accggtacgt ttaacgatcc ttccagacct tcaaccaccg
    22801 ctttcggtac gttatcccac tgatagagat cggtgccgac tgcgttttgc cctacagcca
    22861 catagcccgc ttcaatctta ttgcgataat cgttacggaa ccaggtcacg cccgccagcc
    22921 agccgtcgcg tttgaactcc agaccaatct ccttgttgat gctggtttct gctttcaggt
    22981 catcattacc ttgcagatag cagccgcccg cgctggcata gcagccctga cctttactgt
    23041 agagaatgta gtttgggtta gtctggtaca gactcggcgc tttataggcg cgggcgatgc
    23101 ccattttcag cgtgaagtca tcgcctaaac cttgcgatat gttcagcgcc gggctccagt
    23161 tattgccgac aatactgtga tgatcgaaac gcagccccgg cgttacgatg gtgctgtcag
    23221 tcagctccat attgttttcg gcaaacagcg agaaaatttc tgcttttgaa tatgggctac
    23281 ggtcggtggc actcacacca tcaatagcac cgccggtatt cgttccggtc agcgcctggg
    23341 tgttggaact taagtccttc atccgttgct gattccactc tgtacccagc gtcagcgttt
    23401 ggtttacgag gaaatcaatc ggcaggttaa cttcgctgtg cagcatcacg tcatcaagat
    23461 cgatatctac aaaatcctgt gccgcttttt cgttaaattt cccttcggta ccgcccgcca
    23521 gaccttccgg aatacgcgag ttacgggtgt gttcgtactg cacccagttg ctggtggtca
    23581 cgccgttatc ccagccaccg ttccaggtca gcgagtagtt ctggcgatac aggcggttgg
    23641 tttcatcgcc atatttcgat cgagtgtaag cgtcagagtt ggtgttctgc gtatcacccg
    23701 catacaggtt accctggcgg ctgtagcccg cttccagttc gagtgactgc agaggcgcga
    23761 agtcccagcg caccacgcca ttaatatctt tgttgatcac cccttcgcgc ccggctggta
    23821 acgtcgtggc atacgttccg gcacgcgcgg actgatggcc ctggttgata tcccacgcgt
    23881 cagcctgggt tttgtcgagg ttgccataca agcggaagct gaattcgtcg cccagcggac
    23941 cggtcaggct gaagttggta cgtttggtgg caccttcctc tttatgttct ggcgcattga
    24001 aataagcatc ccaggagccg tgccactcac cactgccttt tttggtaatg atgttaacca
    24061 cgccgcccgc cgcgccgttg ccataacgcg cagctgccgg accacgcaga acttcaatac
    24121 gttcaatcat ttcaggcggc acccaggagg tatcaccacg ggtatcgcgc tcgccacgcc
    24181 agccctgacg cacagagtta cggctgctta ccggcttgcc gtcaatcaaa atcagcgtgt
    24241 tttccggacc catgccgcga atatcaatct ggcggttatt accacgctga ccactggtgg
    24301 aattaccggt caggttaacg ccaggcatgg tacgaatgat ctccgataca tcgcgggcaa
    24361 ccgggttttt gcggatttca tctgcggtaa tggtcgaaac gccaggcgcc tgcaagttct
    24421 gctcggcggc ggtaacgaca atggtatcgt catgtgaaac aggagtatcg gtcggctctt
    24481 gtgcctgtgc taccccataa atccccagat tgaccaacaa ggccagggaa tgaatcttct
    24541 tgttcattgt tttattcctg catttttgcc acgaattgca actgtcgggc atggtcgcca
    24601 tcaacacgac gcatcccgct accgcgaaaa cctttgatcc tgaaagacac gcagtgcagc
    24661 tgattaatta atgtccgcgc ttcccacggc gcgccattac gctattgcaa atgcaaatag
    24721 ttatcaataa tattatcaat atatttctgc aatcaatgaa aaattgcaca gtaaacatgg
    24781 ggttatggtg tgacggcgtt aaaagtagga agtgagagct ggtggcagtc gaaacatggc
    24841 ccggaatggc aacgtctgaa tgacgaaatg tttgaggtca ctttctggtg gcgtgatccc
    24901 cagggttctg aagaatactc gacgataaag cgcgtatggg tctatatcac tggcgtgacc
    24961 gatcaccatc agaacagcca gccccggtcg atgcagcgaa ttgcaggaac cgatgtttgg
    25021 cagtggacga cacaactcaa tgccaactgg cgtggcagct actgctttat tcctactgaa
    25081 cgcgatgaca ttttttctgc accatccccc gatcgcctcg aattacgcga aggctggcga
    25141 aaactattac cccaggcgat agccgatccg ctgaatccac aaagctggaa aggcgggcga
    25201 gggcacgctg tttctgcact cgaaatgccg caagcgtctc tgcaaccggg atgggattgt
    25261 ccgcaagcgc cagaaacgcc tgccaaagaa attatctgga aaagtgaacg gttgaaaaat
    25321 tcacgccgtg tatggatttt taccaccggc gatgcaacag cagaagaacg cccgctggca
    25381 gttttgcttg atggcgaatt ttgggcgcag agtatgcccg tctggcctgc gctgacttcg
    25441 ctgacccatc gtcggcaact tccgcccgcc gtgtatgtgt tgatcgacgc tatcgacacc
    25501 acgcaccgcg cccacgaact gccgtgtaat gcggatttct ggctggcagt acagcaagag
    25561 ttattacccc aggtgaaagc tattgcccct tttagcgatc gcgccgatcg caccgtggtc
    25621 gccggacaga gttttggtgg gctttccgcg ctgtatgccg gactgcactg gcctgaacgc
    25681 tttggctgtg tattaagcca gtcaggttcg tactggtggc cgcatcgggg cgggcaccaa
    25741 gagggcgtgt tacttgaaca gctaaaaact ggtgaagtta gcgccgaagg tctgcgcatt
    25801 gtgctggaag cgggtgttcg cgagccgatg atcatgcagg ccaatcaggc gctgtatgcg
    25861 caattacacc ccctaaaaga atccattttc tggcgtcagg ttgacggcgg acatgatgcg
    25921 ctttgttggc gcggtggctt gatgcagggg ctaatcgacc tctggcaacc acttttccat
    25981 gacaggagtt gaatatggca ttcagtaatc ccttcgatga tccacaggga gcgttttaca
    26041 ttttgcgcaa tgcgcagggg caattcagtc tgtggccgca acaatgcgcc ttaccggcag
    26101 gctgggacgt tgtgtgtcag ccgcagtcac aggcgtcctg ccagcagtgg ctggaagccc
    26161 actggcgtac tctgacaccg gcgaatttca cccagttgca ggaggcacaa tgagccagca
    26221 tttacctttg gtcgccgcac agcccggcat ctggatggca gaaaaactgt cggatttacc
    26281 ctccgcctgg agcgtggcgc attacgttga gttaaccgga gaggttgatg cgccactact
    26341 ggcccgcgcg gtggttgccg gactagcgca agcagatacg ttgcggatgc gttttacgga
    26401 agataacggc gaagtctggc agtgggtcga tgatgcgcag acgttcgaac tgccagaaat
    26461 tatcgacctg cgaaccaaca ttgatccgca cggtactgcg cgggcattaa tgcaggcgga
    26521 tttgcaacaa gatctgcgcg tcgatagtgg taaaccactg gtctttcatc agctgataca
    26581 ggtggcggat aaccgctggt actggtatca gcgttatcac catttgctgg tcgatggctt
    26641 cagtttcccg gccattacac gccagatcgc caatatttac tgcgcatggc tgcgtggcga
    26701 accaacgcct gcttcgccgt ttacgccttt cgctgatgtc gtggaagagt atcagcagta
    26761 ccgcgaaagt gaagcctggc agcgtgatgc ggcattctgg gcggaacagc gtcgtcaact
    26821 gccaccgccc gcgtcacttt ctccggcacc tttaccgggg cgcagcgctt cggcagatat
    26881 tttgcgcctg aaactggaat ttaccgacgg ggaattccgc cagctggcta cgcaactttc
    26941 aggtgtgcag cgtaccgatt tagcccttgc gttggcagct ttgtggctgg ggcgattgtg
    27001 caaccgcatg gactacgctg ccggatttat ctttatgcgt cgactgggct cggcggcgct
    27061 gacggctacc ggacccgtgc tcaacgtttt gccgttgggt attcacattg cggcgcaaga
    27121 aacgctgccg caactggcaa cccgactggc ggctcaactg aaaaaaatgc gccgccatca
    27181 acgctacgac gccgaacaaa ttgtccgtga cagtgggcga gcggcaggag aggaaccgct
    27241 gttcggtccg gtactcaata tcaaggtatt tgattaccaa ctggatattc ctggtgttca
    27301 ggcgcaaacc cataccctgg caaccggtcc ggttaatgac cttgaactgg ccctgttccc
    27361 ggatgaacac ggtgatttga gtattgagat cctcgccaat aaacagcgtt acgatgagcc
    27421 aacgttaatt caacacgctg aacgcctgaa aatgctgatt gcgcagttcg ctgcggatcc
    27481 gtcgctgttg tgcggcgatg tcgatattat gctgccaggc gagtatgcgc agctggcgca
    27541 gatcaacgcc actcaggttg agattccaga aaccacgctg agcgcgctgg tggcagaaca
    27601 agcggcaaaa acaccggatg ctccggcgct ggcagatgcg cgttaccagt tcagctatcg
    27661 ggaaatgcgc gagcaggtgg tggcgctggc gaatctgctg cgtgagcgcg gcgttaaacc
    27721 gggcgacagc gtggcggtgg ctctaccgcg ctcggtcttt ttgaccctgg cgctacatgc
    27781 gatagttgaa gctggtgcgg cctggctacc gttggatacc ggctatccgg acgatcgcct
    27841 gaaaatgatg ctggaagatg cgcgtccttc gctgttaatc accactgacg atcaactgcc
    27901 gcgctttagc gatatcccta atttaaccag cctttgctat aacgccccgc ttacaccgca
    27961 gggcagtgcg ccgctgcagt tttcacaacc gcaccacacg gcttatatca tcttcacctc
    28021 cggttccacc ggcagaccaa aaggggtaat ggtcgggcag acggctatcg ttaatcgcct
    28081 gctttggatg caaaaccatt acccgcttac aggcgaagat gtcgttgccc aaaaaacgcc
    28141 gtgcagtttt gatgtctcgg tgtgggagtt tttctggcca tttattgccg gggcaaaact
    28201 ggtgatggct gaaccggaag cgcaccgcga cccgctcgct atgcagcaat tctttgccga
    28261 atatggcgta acgaccacgc actttgtgcc gtcgatgctg gcggcatttg ttgcatcgct
    28321 gacgccgcaa accgctcgcc agagttgcgc gacgttgaaa caggttttct gtagtggtga
    28381 agccttaccg gctgatttat gccgcgaatg gcaacagtta tccggcgcgc cgttgcataa
    28441 tctatatggc ccgacggaag cggcggtaga tgtcagttgg tatccggctt ttggcgagga
    28501 actggcacag gtgcgcggca gcagtgtgcc gattggttat ccggtgtgga atacgggcct
    28561 gcgcattctc gatgcgatga tgcatccggt gccgccgggt gtggcgggag atctctatct
    28621 caccggtatt caactggcgc aggggtatct tggacgaccc gatctgacag ccagccgctt
    28681 tattgccgat ccttttgccc caggtgaacg gatgtaccgt accggcgatg tggctcgctg
    28741 gctggataac ggcgcagtgg agtacctcgg gcgcagtgac gatcagctaa aaattcgcgg
    28801 gcagcgtatc gaactgggcg aaattgatcg cgtgatgcag gcgctgccgg atgtcgaaca
    28861 agccgttacc cacgcctgtg tgattaacca ggcggcagcc actggtggtg atgcgcgtca
    28921 gttggtgggc tatctggtgt cgcaatcagg tctgccgttg gataccagcg cattacaggc
    28981 gcagcttcga gaaacattgc caccacatat ggtaccggtg gttctgctgc aacttccaca
    29041 gttaccactt agcgccaacg gcaagctgga tcgcaaagcc ttaccgttgc ctgaactgaa
    29101 ggcacaaacg ccggggcgtg tgccgaaagc gggcagtgaa acgattatcg ccgcggcatt
    29161 ctcgtcgttg ctgggttgtg acgtgcagga tgccgatgct gatttctttg cgcttggcgg
    29221 tcattcgcta ctggcaatga aactggcagc gcagttaagt cgacagtttg ctcgtcaggt
    29281 gacgccgggg caggtgatgg tcgcgtcaac cgtcgccaaa ctggcaacga ttattgatgg
    29341 tgaagaagac agcacccagc gcatgggatt cgaaaccatt ctgccgttgc gtgaaggtaa
    29401 tggtccgacg ctgttttgtt tccatccggc atccggtttt gcctggcagt tcagcgtgct
    29461 ctcgcgttat ctcgatccac aatggtcgat tatcggcatt cagtcgccgc gccctcatgg
    29521 ccccatgcag acgtcagcaa atctggatga agtctgcgaa gcgcatctgg caacgttact
    29581 tgaacaacaa ccgcacggcc catattacct gctggggtat tcacttggcg gtacgctggc
    29641 gcagggcatt gcggcgcgac tgcgtgcccg tggcgaacag gtggcatttc ttggcttgct
    29701 ggatacctgg ccgccagaaa cgcaaaactg gcaggaaaaa gaagccaatg gtctggaccc
    29761 ggaagtgctg gcggagatta accgcgagcg cgaagccttc ctggcggcac agcagggaag
    29821 tacttcaacg gagttgttta ccaccattga aggcaactac gctgatgctg tgcgcctgct
    29881 gacgacagct catagcgtac cgtttgatgg taaagcgacg ctatttgttg ccgaacgcac
    29941 gcttcaggaa ggtatgactc ctgaacaggc ctgggcaccg tggattgctg agctggatat
    30001 ctatcgtcag gattgtgcgc atgtggatat tatttccccg gagtatttta aagaaatagg
    30061 accactaata aatacacaaa ttaataacta accgtacgtt agtaaagaac ttgccata
//
LOCUS       DADSZD010000041        29127 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.041, whole genome shotgun sequence.
ACCESSION   DADSZD010000041 DADSZD010000000
VERSION     DADSZD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.041"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(210..755)
                     /gene="orn"
                     /locus_tag="J1Z28_001639"
     CDS             complement(210..755)
                     /gene="orn"
                     /locus_tag="J1Z28_001639"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBA7716481.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            850..1902
                     /gene="rsgA"
                     /locus_tag="J1Z28_001640"
     CDS             850..1902
                     /gene="rsgA"
                     /locus_tag="J1Z28_001640"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBA7716482.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMKFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            1999..2967
                     /gene="psd"
                     /locus_tag="J1Z28_001641"
     CDS             1999..2967
                     /gene="psd"
                     /locus_tag="J1Z28_001641"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBA7716483.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            2989..6312
                     /gene="mscM"
                     /locus_tag="J1Z28_001642"
     CDS             2989..6312
                     /gene="mscM"
                     /locus_tag="J1Z28_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBA7716484.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLIGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKDGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6462..7964)
                     /gene="yjeM"
                     /locus_tag="J1Z28_001643"
     CDS             complement(6462..7964)
                     /gene="yjeM"
                     /locus_tag="J1Z28_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBA7716485.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8183..9160)
                     /gene="epmA"
                     /locus_tag="J1Z28_001644"
     CDS             complement(8183..9160)
                     /gene="epmA"
                     /locus_tag="J1Z28_001644"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBA7716486.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9485..11293
                     /gene="frdA"
                     /locus_tag="J1Z28_001645"
     CDS             9485..11293
                     /gene="frdA"
                     /locus_tag="J1Z28_001645"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBA7716487.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11286..12020
                     /locus_tag="J1Z28_001646"
     CDS             11286..12020
                     /locus_tag="J1Z28_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HBA7716488.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12031..12426
                     /gene="frdC"
                     /locus_tag="J1Z28_001647"
     CDS             12031..12426
                     /gene="frdC"
                     /locus_tag="J1Z28_001647"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBA7716489.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12437..12796
                     /gene="frdD"
                     /locus_tag="J1Z28_001648"
     CDS             12437..12796
                     /gene="frdD"
                     /locus_tag="J1Z28_001648"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBA7716490.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12859..13992
                     /gene="blaEC"
                     /locus_tag="J1Z28_001649"
     CDS             12859..13992
                     /gene="blaEC"
                     /locus_tag="J1Z28_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBA7716491.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYAGIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSADEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPFDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14081..14614
                     /locus_tag="J1Z28_001650"
     CDS             14081..14614
                     /locus_tag="J1Z28_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBA7716492.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14611..14928)
                     /locus_tag="J1Z28_001651"
     CDS             complement(14611..14928)
                     /locus_tag="J1Z28_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HBA7716493.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15111..15257)
                     /gene="ecnB"
                     /locus_tag="J1Z28_001652"
     CDS             complement(15111..15257)
                     /gene="ecnB"
                     /locus_tag="J1Z28_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBA7716494.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15368..15493)
                     /gene="ecnA"
                     /locus_tag="J1Z28_001653"
     CDS             complement(15368..15493)
                     /gene="ecnA"
                     /locus_tag="J1Z28_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBA7716495.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15545..16111)
                     /gene="efp"
                     /locus_tag="J1Z28_001654"
     CDS             complement(15545..16111)
                     /gene="efp"
                     /locus_tag="J1Z28_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBA7716496.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16153..17181
                     /gene="epmB"
                     /locus_tag="J1Z28_001655"
     CDS             16153..17181
                     /gene="epmB"
                     /locus_tag="J1Z28_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBA7716497.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            17416..18285
                     /locus_tag="J1Z28_001656"
     CDS             17416..18285
                     /locus_tag="J1Z28_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716498.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(18335..18688)
                     /locus_tag="J1Z28_001657"
     CDS             complement(18335..18688)
                     /locus_tag="J1Z28_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBA7716499.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(18826..20472)
                     /gene="groL"
                     /locus_tag="J1Z28_001658"
     CDS             complement(18826..20472)
                     /gene="groL"
                     /locus_tag="J1Z28_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBA7716500.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVAAAVEELKTLSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(20516..20809)
                     /locus_tag="J1Z28_001659"
     CDS             complement(20516..20809)
                     /locus_tag="J1Z28_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004391824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBA7716501.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            21085..22341
                     /gene="yjeH"
                     /locus_tag="J1Z28_001660"
     CDS             21085..22341
                     /gene="yjeH"
                     /locus_tag="J1Z28_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBA7716502.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALELNLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(22357..22833)
                     /gene="fxsA"
                     /locus_tag="J1Z28_001661"
     CDS             complement(22357..22833)
                     /gene="fxsA"
                     /locus_tag="J1Z28_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBA7716503.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            23170..24606
                     /gene="aspA"
                     /locus_tag="J1Z28_001662"
     CDS             23170..24606
                     /gene="aspA"
                     /locus_tag="J1Z28_001662"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBA7716504.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            24724..26025
                     /locus_tag="J1Z28_001663"
     CDS             24724..26025
                     /locus_tag="J1Z28_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBA7716505.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            26141..26479
                     /gene="cutA"
                     /locus_tag="J1Z28_001664"
     CDS             26141..26479
                     /gene="cutA"
                     /locus_tag="J1Z28_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBA7716506.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            26455..28152
                     /locus_tag="J1Z28_001665"
     CDS             26455..28152
                     /locus_tag="J1Z28_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBA7716507.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHTKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGETHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            28189..28764
                     /locus_tag="J1Z28_001666"
     CDS             28189..28764
                     /locus_tag="J1Z28_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7716508.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            28871..28946
                     /locus_tag="J1Z28_001667"
     tRNA            28871..28946
                     /locus_tag="J1Z28_001667"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28904..28906,aa:Phe,seq:gaa)
BASE COUNT         7158 a         7599 c         7346 g         7024 t
ORIGIN      
        1 tactacttaa ttttgctttc gtaattttcg catttctgcg tcgttacggg aggcgtatta
       61 tacgagaaat ctttccctct gcaacccccc taaaaaattt tttttgagtt tttatctcaa
      121 gtgctgaaat tatcagcaag acgttcaatt tagcgtcaaa atcacgccgc cgcaagtgga
      181 ttccggcgct tacgtgacca ggaaaaattt tacagcttga taaaatgctc gcggtagtaa
      241 gccaactccg ccaccgattc acggatatca tccatcgcct gatgcgtccc ctgcttggta
      301 aagccgtcaa gaatttccgg cttccagcgg cgcgccagct ctttcagggt gctaacatcg
      361 agatagcggt agtggaagta ggcttccagc tccggcatgt atttaaacag gaagcgacgg
      421 tcctgaccga tgctgttacc acaaatcggc gattttcccg caggcaccca ctgttttaag
      481 aattcgagcg ttgccagttc agcttcacga tcgcccatcg tgctcgcttt cacacgctct
      541 accagcccgc tggcggtatg ggtgcgcacg ttccagtcat ccatcagcgc cagctgttca
      601 tcggactgat gtactgcaat ggtcggccct tctgccagaa tattcaggtt ggcatcggtc
      661 accagcgtgg caatctcaat aatgcgatcg cgctcgggat ccagaccggt catctcaaga
      721 tcgatccaaa tcaggttgtt ttcattggca ctcatgctat tttccaccca ttcacagtga
      781 ctatattgat ccacaattgc gtgtatcata gatgttttgc ccatcagggg cgaccaggag
      841 tctgtacgat tgagtaaaaa taaactctcc aaaggccagc agcgccgcgt gaacgccaat
      901 caccagcgtc gtcttaaaac gtctaaggag aagcccgact acgacgacaa tctgtttggc
      961 gagcctgatg aaggtatcgt catcagccgc tttggtatgc acgctgatgt ggaatccgcc
     1021 gatggcgacg ttcaccgctg caatattcgc cgtactatcc gttcgctggt gaccggcgac
     1081 cgcgtagtct ggcgtccggg taaaccggcg gcggaaggcg taaatgtcaa agggatcgtg
     1141 gaagcggtgc atgagcgcac ttcagtgtta acgcgcccgg acttctacga tggcgtgaaa
     1201 cctattgccg ccaacatcga ccagattgtc attgtctccg ccattttgcc ggagctgtcg
     1261 ctcaatatta tcgaccgtta cctggtggcc tgcgaaacct tgcagattga gccgattatt
     1321 gtgctcaaca aaatagacct gctggacgac gaaggtatga agttcgtcaa cgagcagatg
     1381 gatatctacc gcaatatcgg ttatcgcgta ttgatggttt ccagccatac tcaggatggg
     1441 ctgaaaccgc tggaagaggc gttgaccgga cgtatcagca tttttgccgg gcagtctggc
     1501 gtcggtaaat ccagcctgct gaacgcgctg ctggggctgc aaaaagagat cctgaccaac
     1561 gatgtatctg acaactcggg tctcggtcag cacaccacga ccgccgctcg gctgtatcac
     1621 ttcccgcacg gcggtgatgt gattgactcc cctggtgtgc gtgagttcgg cctctggcac
     1681 ctggagccgg aacaaatcac ccagggcttt gtcgaattcc atgactactt aggtctgtgt
     1741 aaatatcgcg attgcaaaca cgataccgat ccgggctgcg ctatccggga agcggttgag
     1801 gaagggaaaa tcgcggaaac ccgtttcgaa aactatcacc gtattctgga aagcatggcg
     1861 caggtaaaaa cgcgtaaaaa cttttctgat acggatgact gacaattaag ctaaccgtcg
     1921 ttagaatcgt cccctttttt tcaggatccg gcatgtaggc cggatcagga acgacaaaac
     1981 aatggcctgg aggctacctt gttaaattca tttaaacttt cgctacagta cattctgccg
     2041 aaactatggc ttactcgcct ggcgggttgg ggcgcaagca agcgggcagg atggctgaca
     2101 aaactggtta tcgatctgtt cgttaaatac tacaaggtcg acatgaaaga ggcgcaaaag
     2161 ccggacaccg ccagctaccg cacctttaac gaattctttg tccgtccgct gcgtgacgaa
     2221 gtacgcccaa tcgataccga tccgaatgta ctggttatgc ctgccgatgg cgttatcagc
     2281 cagttgggta aaatcgaaga agataaaatc ctgcaagcca aaggccacaa ctacagcctc
     2341 gaagccctgc tggcaggcaa ctatctgatg gcggatctct tccgtaacgg tacgtttgtg
     2401 accacttacc tctccccacg tgactaccac cgcgtacaca tgccgtgtaa cggtattctg
     2461 cgtgaaatga tctacgtgcc gggcgatctc ttctccgtta accatctcac ggctcagaac
     2521 gtgccgaatc tgtttgcccg taacgaacgc gtgatctgcc tgttcgatac cgaatttggc
     2581 ccgatggcgc agattctggt cggtgcgacg attgtaggca gcattgagac ggtctgggcg
     2641 ggcaccatta cgccgccgcg cgaaggcatc atcaagcgct ggacctggcc tgccggggaa
     2701 aacgacggtt ctgtggcatt gctgaaaggc caggaaatgg gtcgctttaa acttggctct
     2761 accgttatca acctgtttgc accaggtaaa gtgaacctgg ttgagcaact ggaaagcctg
     2821 tctgtcacga aaattggcca gccgctggca gtatctaccg aaacctttgt tacgccagac
     2881 gctgaaccag ccccgcttcc tgctgaagaa atcgaggcag aacacgacgc cagcccattg
     2941 gttgacgaca aaaaagacca ggtctaatcc atcaaaggaa acgctgacgt gcgcctgatt
     3001 atcacttttc tgatggcctg gtgcctcagt tggggggcgt acgccgcgac ggcccccgat
     3061 agcaaacaaa tcacccagga actggagcag gcaaaagcgg cgaaacctgc gcagccggaa
     3121 gtcgtagagg cgctccagtc tgccttaaat gcgcttgagg aacgaaaagg ttcccttgag
     3181 cgcatcaagc aatatcaaga agtcattgat aattatccga aactttccgc cactctgcgc
     3241 gcgcagttaa acaacatgcg tgacgagcca cgcagcgtgt cgccgggaat gtctaacgat
     3301 gcgctgaatc aggaaattct ccagatcagc agtcagttgc tggataaaag ccgtcaggcc
     3361 cagcaagagc aggagcgcgc ccgcgagatt gccgattcgc tgaatcaact gccgcaacag
     3421 caaaccgatg cccgccgtca gttgaatgaa atcgagcgcc gcctggggac acttatcggc
     3481 aataccccgc tcaatcaggc acaaaatttc gcgttgcagt ctgactccgc acgtctgaaa
     3541 gcgctcgttg atgaactgga gctggcgcag ctatctgcca ataaccgcca ggaattagcg
     3601 cgcttgcgct ctgagctggc ggaaaaagag agccagcagc tggatgcata tttgcaagca
     3661 ctacgcaacc agttgaatag tcaacgccaa ctggaagcgg aacgggcgct ggaaagtacc
     3721 gagcaactgg cggaaagtag cgccgatttg ccaaaagata tcgtcgccca gtttaaaatt
     3781 aaccgcgaac tatcggcggc gctgaatcaa caggcgcagc ggatggatct cgttgcctcg
     3841 caacagcgtc aggctgccag ccagacgtta caggtccggc aggcgttgaa tacgctgcgt
     3901 gaacagtcgc aatggctggg atcatctaac ctgctcggcg aagcgttgcg ggcgcaggtg
     3961 gcacggctac cggaaatgcc aaaaccacaa cagcttgata ccgaaatggc gcagttacgt
     4021 gtgcaacggt tacgttatga ggatctgctt aataaacagc cgctgctacg gcaaatccat
     4081 caggccgacg gtcagccact gaccgctgag caaaaccgta ttctggaagc acagctacgc
     4141 actcagcgtg agttgctgaa ctcattgttg cagggtggcg acacgctact gctggagctg
     4201 accaagctga aagtctccaa cgggcaactg gaggatgcgc tgaaagaggt gaacgaagcg
     4261 acgcaccgct atctgttctg gacctctgac gtgcgcccga tgaccatcgc ctggccgctg
     4321 gaaatcgccc aggatctgcg tcgtctcatt tcgctggaca ccttcagtca gttgggcaaa
     4381 gccagtgtga tgatgctgac cagtaaagag acgattttgc cgctgtttgg cgcgttgatt
     4441 ctggtcggtt gcagtattta ctcgcgccgc tatttcaccc gttttcttga acgttcggcg
     4501 gcgaaagtcg gcaaagtgac tcaggatcac ttctggctga cgttacgcac tcttttctgg
     4561 tcgattctcg tcgcgtcacc gctgcctgtg ctgtggatga cgctgggtta cggcttgcgc
     4621 gaggcgtggc cttatccgct ggcggtcgcg attggtgatg gcgtaacagc caccgtgccg
     4681 ctgctgtggg tagtgatgat ttgcgccacc tttgctcgcc cgaacggctt gtttatcgct
     4741 cattttggct ggccgcgcga acgtgtttcc cgtgggatgc gttactacct gatgagcatc
     4801 gggcttattg tgccgctgat tatggcgctg atgatgttcg ataacctcga cgaccgcgaa
     4861 ttttccggtt cgctgggacg gctttgcttt atcctcattt gcggtgcgct ggcggtggtc
     4921 accctcagcc tgaaaaaggc cgggatcccg ctgtatctca acaaagatgg cagcggcgac
     4981 aacattacca accatatgct gtggaacatg atgattggcg cacctctggt tgccattctg
     5041 gcgtcggcgg tgggttatct ggcaacggcg caggcgctgt tagcgaggct tgaaacctcg
     5101 gttgccatct ggttcctgct actggtggtt tatcacgtta tccgccgctg gatgctgatc
     5161 cagcgccgca ggctggcgtt tgaccgggca aagcatcgcc gggcagagat gttagcgcag
     5221 cgtgcgcgtg gcgaagagga agcgcatcat cacagtagcc cggaaggagc aattgaagtc
     5281 gatgaaagcg aagtcgatct cgatgccatc agtgcgcaat ccttgcggct ggtgcgctca
     5341 attttgatgt tgatcgccct gctttcggtc attgtgctgt ggtcagaaat ccattccgct
     5401 ttcggcttcc tcgaaaatat ttcgctgtgg gatgtcactt ccacggtaca gggcgtagaa
     5461 agcctggagc cgattaccct cggtgcggta ctgattgcca ttctggtgtt tatcatcacc
     5521 acgcagctgg tgcgcaacct gcccgcgctg ctggaactgg cgattctgca gcacctggat
     5581 ttaacgccgg gtacgggcta cgccatcacc accatcacca aatatctgct gatgctgatt
     5641 ggcgggctgg tcggcttctc gatgattggt attgagtggt cgaaattgca gtggctggtc
     5701 gccgcgctcg gtgttggtct cggttttggt ttgcaggaaa ttttcgccaa ctttatctct
     5761 ggcctgatta tcctgttcga aaaaccgatt cgcattggcg atacggtaac gatccgcgat
     5821 cttaccggta gtgtgacaaa aattaacacc cgcgccacca ccatcagcga ctgggaccgc
     5881 aaagaaatta tcgtgccgaa caaggcgttt attaccgagc agtttatcaa ctggtcgctc
     5941 tctgactcgg tcacgcgcgt ggtgttgacg attccggccc ctgccgatgc caacagtgaa
     6001 gaagtgacgg aaatcctgct caccgcagcg cgtcgctgct cgctggtaat cgataaccca
     6061 gcaccggaag tcttcctggt ggatctgcaa caggggattc aaattttcga gctgcgtatt
     6121 tacgccgctg agatgggtca ccgtatgcca ctacgccatg agattcacca gctgattctg
     6181 gcaggattcc atgcccacgg tattgatatg ccattcccgc ccttccagat gcgtttggaa
     6241 agcctcaacg gtaaacaaac ggggagaaca ttaacttctg cgggcaaagg tcgtcaggcg
     6301 ggaagtttgt aatgcatgaa gctgccaaat gatgtcaacg tggctcattt agcagcttaa
     6361 catcatcttc agttatcact ggccccggag agtatcatcc atacgcatta cttcctgcaa
     6421 ctcatcgccg gagagacgat gagttgcagg atttcatatg atcagtcgag agctaactca
     6481 ggtttgttcg ccatgcgact gcaatagttc tggtaaatcg ccatcgctaa cagcgagaag
     6541 aagacagggc cgccaatcat ccacaatgtg ctgtcccagt ctccagcttc aaccacaggt
     6601 tgaatgatgg tgaagacgtt cgcaaatgtc accaccagta cgacaaccac tgttgcaatc
     6661 attgccgaca tatgcgtttt gaaaatcaca aacggtctgt cgagatcctg acgtgcttta
     6721 aaaaatggga aagccagcgc gaggaacagg taaggaagcg tcatagacac gttcgccatc
     6781 agcgtcagct tgttaaagaa cgccgatgcg gtaccgccac caaacgaaac cagcaggatg
     6841 aagacagtaa ccaacccaca ctgcatccac atggcgatag acggcatccc catcgcattc
     6901 aggcgcgtca tcggttccgg ccacaatgct ttcggcgtcc cctggatgat ggctttcagc
     6961 ggtgaatagc aaagtgtaaa gaacgcaccg gtataagcga ggaacatcga cagtccggta
     7021 atacgcgcaa accatacgcc cagcgacaat gacgcttcag gtgacaaatg cagtgcgtta
     7081 cccagcgtca ccccgaggct cttcatcagc acataggtaa tattgccgag gttaacggaa
     7141 ccattactta atacctgctg ccagtttgtg ctgacgcccc ataaaaatat tgccagcgaa
     7201 taaccgattg aaataacaat agcggcaaaa acaatacctt tggcaaagtt cttttctgga
     7261 ttttccgttt tatcaaccag accaccaacc gcttcaattc caccataggc aaaaatggca
     7321 aataccacaa acgatagcat tgccagaccg gactgataac ctgggttcgg tgatgccagg
     7381 aaattaatat cctgcgcgaa atgcccacca tttaataaca aaatagtaat gcttactaac
     7441 agcaatacta aattcagaca cattactgca ataccgccca ccgcagtaat gcgggcaatt
     7501 ttattaatcc cttttgaagc aacgacggtg accagaatca tccatgccac tgccagcaga
     7561 ccaaccacct gcgtaggctc cagtccggca atatgccagt gctgggtcat gtcgctacca
     7621 tagaggaatg ttgagaacgg tacccaaact ttcgcggagg tactcaccat ccagatgata
     7681 taagaggaaa accacataaa cgtaccaatg aaggcaaaac gtggtccgac actattattc
     7741 atccaggaat agataccgcc ttcttctttg cgataagcag ctcccatttc agccatcatt
     7801 aaggcgaatg gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa
     7861 cccattaagt aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc
     7921 aatatgagtc ctatcagact catctttttt atcgagtgag gcatgaaacc atccttcatt
     7981 tcagcgcgtg tataaaacgc gcccactgaa ctgcactgcc caacattgag cgggcagcgt
     8041 aaaaaccaat acagaattga tacctggatg atatcaaata tccagtctta aattgtggtc
     8101 tttggcggaa aaatgaaaac atatgctaca gaatggcgct tatcacgcca ttcttcgctg
     8161 ttaattcagt aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca
     8221 gtgtctccgc gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac
     8281 agtcaggcat accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca
     8341 aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat
     8401 ccgtcaattc atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt
     8461 cagcgacccg atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt
     8521 ggtacacaaa ggtcggtttt tctttgccaa tatttggctc tacgccaaag gtaaacagca
     8581 attgtagaag cgtgtcgcgg tcttcttcag tatcggcaac gttgctcaaa tccagtttcg
     8641 ctgccacttc ccgcagttgc gttttgtcgg cagagagtgg gtcaatttcc agataacgca
     8701 agaaagcttg ttgataagaa aggctttctg ctgccggaca gtccagcacc tgctgcaaga
     8761 gatcgtccac ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactcaagca
     8821 tagtgaactc agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct
     8881 ggaataccgg cccacaaccg gcaaccagca ggcgcttcat atggtattcc gggctggtca
     8941 ttaaccagag attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga
     9001 ccaaatgaat atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc
     9061 cacgatcggc aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag
     9121 gaatggatgc gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg
     9181 acaagggcac gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaataaaa
     9241 caacggtggg cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat
     9301 cttcagtgat aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac
     9361 ttatccatca gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc
     9421 gtaactttca ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa
     9481 tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg
     9541 tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt
     9601 atacccgatg cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga
     9661 tcatgacagc ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga
     9721 gcaggatgtc gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact
     9781 gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg
     9841 catgaaaatc gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac
     9901 gctgttccag acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct
     9961 ggatattctg gttgatgatg gtcatgttcg cggcctggta gcaatgaaca tgatggaagg
    10021 cacgctggtg cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt
    10081 ttatcgttac aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag
    10141 ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gcctaccagg
    10201 ttccggtatc ctgatgaccg aaggctgccg cggtgaaggt ggtattctgg tcaacaaaaa
    10261 tggctaccgt tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa
    10321 aaacaaatat atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg
    10381 gcgtaaaggc aacaccatct ccacgccacg tggcgatgtg gtttacctcg acctgcgtca
    10441 ccttggcgag aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta
    10501 cgttggcgtc gatccggtta aagaaccgat tccggtacgt ccgaccgcac actacaccat
    10561 gggcggtatc gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg
    10621 tgaatgttcc tctgttggtc tgcacggtgc gaaccgtctg ggctccaact cgctggcgga
    10681 actggtggtc ttcggtcgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg
    10741 taatggcaac gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga
    10801 tctggttaac caggatggcg gcgaaaactg ggctaagatc cgcgacgaaa tgggcatggc
    10861 catggaagaa ggctgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa
    10921 gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc accgacactt ccagcgtgtt
    10981 caacaccgac ctgctctaca ccattgagct gggccacggt ctgaacgttg ctgaatgtat
    11041 ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgcg caccagcgtc tggacgaagg
    11101 ttgcaccgag cgtgacgacg tcaacttcct caaacacacc ctcgccttcc gcgatgctga
    11161 tggcacgact cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg
    11221 tgtttacggt ggcgaagcgg atgcagccga taaggcggaa gcagccaata agaaggagaa
    11281 ggcgaatggc tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg
    11341 ataccgcacc gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg
    11401 atgcgctggg ctacatcaaa gacaacctgg caccggacct gagctaccgc tggtcctgcc
    11461 gtatggcgat ttgtggctcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta
    11521 aaaccttcct gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga
    11581 ttgaacgcga tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac
    11641 cgtacatcat cggcaacgcc cgcaccgcgg atcagggtac taacatccag accccggcgc
    11701 agatggcgaa gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt
    11761 gcccgcagtt tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc
    11821 gttataacga agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc
    11881 agaacggcgt atggagctgt actttcgtgg gctactgctc cgaagtctgc ccgaaacacg
    11941 tcgatccggc tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg
    12001 cgaccctgaa accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg
    12061 ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa
    12121 ggcacggcgg ttccggctgt gtggttcagc attgaactga ttttcgggct gtttgccctg
    12181 aaaaatggcc cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg
    12241 atcattaacc tgatcactct ggcggcagcc ctgctgcaca ccaaaacctg gtttgagctg
    12301 gcaccaaaag cagccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc
    12361 aaaagtctct gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac
    12421 tggtaaggag cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg
    12481 gggcctcttc ggggccggtg gtatgtggag cgccatcatt gcgccggtga tgatcctgct
    12541 ggttggtatt ctgctgccgc tagggctgtt tccgggtgat gcactgagct acgagcgcgt
    12601 tctggcgttc gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc
    12661 gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtacc
    12721 tgcgggtaaa tgggttttct acggtctggc tgctatcctg acagttgtca cactgattgg
    12781 tgtcgttaca atctaacgca tcgccaatgt aaatctggcc cgcctatggc gggccgtttt
    12841 gtatggaaac cagaccctat gttcaaaacg acgctctgca ccttattaat taccgcctct
    12901 tgctccacat ttgccgcacc tcaacaaatc aacgatattg tgcatcgcac aattaccccg
    12961 cttatagagc aacaaaagat ccccggtatg gcggtagcgg taatttatca gggtaaacct
    13021 tattacttta cctggggcta tgcgggcatc gccaaaaagc agcccgtcac acagcaaacg
    13081 ttgtttgagt taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt
    13141 gctcgagggg aaatcaagtt aagcgatccc acaacaaaat actggcctga acttaccgct
    13201 aaacagtgga atgggatcac actattacat ctcgcaacct acaccgctgg cggcctgcca
    13261 ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg
    13321 cagcctgcat gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc
    13381 ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc aggcgatgaa aactcgtgtc
    13441 ttccagccac tcaaactcaa tcatacatgg attaatgtac cgtccgcaga cgaaaagaac
    13501 tacgcctggg gatatcgcga aggtaaggca gtacatgttt cgccaggggc gttagatgct
    13561 gaagcttatg gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta
    13621 aaaccctttg atatcaatga gaaaatactt caacaaggga tacagctggc acaatctcgc
    13681 tactggcaaa ccggcgatat gtatcagggt ctgggctggg aaatgctgga ctggccggta
    13741 aatcctgaca tcatcattaa cggcagtgac aataaaattg cactggcagc acgccccgta
    13801 aaaccgatta cgcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg
    13861 accggcggat ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg
    13921 ctggctaaca aaaactatcc caatccagcg agagttaccg ccgcctggca gattctcaac
    13981 actctacagt aaaatttcat cgggtccgaa ttttcggacc ttttctccgc ttttccttgc
    14041 tgtcatctac acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt
    14101 tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt tcccctacgc cgccgcgtgg
    14161 cgtgaccgta gtgaataatt tcgacgctaa acgttatctc ggtacctggt atgagattgc
    14221 ccgttttgat caccgctttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg
    14281 tgatgacggc ggcctgaatg tcattaataa aggctataac cctgacagag aaatgtggca
    14341 gcagagtgaa gggaaagcgt actttaccgg agcaccaact cgcgctgcgc tgaaagtgtc
    14401 attctttggt cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca
    14461 tgcgctggtt tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat
    14521 ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag
    14581 taagtttatt tgggtacagc agcccggtag ttaatgagtg ctgagtttca gaccaataat
    14641 ccccaatacg attaacgcca gactcgccag gcgcatcggg ttagcggact caccgagcag
    14701 cacaatgccg gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt
    14761 ccctactggt aacgatttca tcgcccaggc aagtagcgcc agactgacaa tcatcgccgt
    14821 cacggtaata acacttggcg tcaaacgact aaagccgtga gtatatttca ggccaacggc
    14881 ccataccact tccagcagac cagcaataac taagataatc caggacatat caggctccgg
    14941 aaaattgggg ccgtccccgg tgaaaagacg cgtttgcagg tcgtcctgca aagctgatgg
    15001 ttgaacggat attttgccag gtggcagggg aatttcaact attacctatg taactatcgc
    15061 agaaaaaaca aacggcacga cacagctgtc gtaccgttat tgcttattgc ttattgctgc
    15121 gctttcgttg ctgcgccaga aatcgcgtta ccgccatcag aaatgtcttc accaacgcca
    15181 cgcgtggtgt tgcaggcagt taatactgtt gaaagcacca gaacagaaaa gatcgctgca
    15241 attgtcttct tcaccataac gtcttccttt taggtaatgt tatttatgtt tgcctatagc
    15301 aaattaataa tagacaacat cgccaaaaat gatggaaatc tgatgatttt tcggaagaga
    15361 agaaaaatta gctggcagcg cgggagatgg agttgccgag gtgtttgata tcttcgccga
    15421 aaccgcgagc ggtgttacag cccgtaagca gcgtgctggc aagcaaaaca agaacgataa
    15481 ggcgtttcat catctctgac ctgaataagt gatggtgcag cctgcaggcc gcaccacaac
    15541 cgcattactt cacgcgagag acgtattcac cagagcgggt atccactttg atgacttcgc
    15601 cgatttgtac aaacagcgga actttaacca cagcgccagt agacagggta gccggtttgc
    15661 cgccagtacc tgcggtatca cctttcaggc ccggatcggt atcaacgatt tccagttcaa
    15721 cgaagttcgg cggagtaacg gagatcggct gaccattcca cagagttacg atacactctg
    15781 cctgatccag caaccattta gcgttgtcgc caattgcttt tgcgtcagca gacagctgct
    15841 cgaaagtttc gttgttcatg aagtgccaga actcaccgtc gttgtacagg taagtcaggt
    15901 tcatatcgac aacatcagcg ccttcagcgg aatcagtaga tttgaaggtt ttttctacgc
    15961 gagtaccggt cagcagacga cgcagtttaa cgcgagcaaa tgcctggcct ttacccggtt
    16021 ttacgaattc actcgcttca accgcgtaag gttcgccgtc taacatgatt ttaagaccag
    16081 cacgaaaatc gttgctatag tacgttgcca taaggccctc tgaaatttgt taattggtag
    16141 ctaagccaca aaatggcgca tattgtaacc ctaaataccc catccagaga agattggtta
    16201 acgcaacttg ccgatgttgt gaccgatcct gatgaacttc tgcgtctttt gaatatagac
    16261 gcggacgaaa aactgttggc cggacgcagc gccaaaaagc tgtttgccct gcgtgtgccc
    16321 cgctcattta tcgatcgcat ggagaaaggc aatccgaacg atccgctttt gcgtcaggta
    16381 cttacctcgc aagatgagtt tgtcgtcgcg cccggattct ccaccgaccc gctggaagaa
    16441 cagcatagcg tagtgcctgg tttgttgcat aaataccaca accgggcgct tttgctggtc
    16501 aaaggcggct gcgcggtaaa ttgccgctat tgcttccgtc gccacttccc ctatgccgaa
    16561 aatcagggca ataagcgtaa ctggcagact gcacttgagt atgttgctgc gcatccggaa
    16621 ctggacgaga tgattttctc cggcggcgat ccgctgatgg cgaaagatca cgagctggac
    16681 tggttgctca cacaactgga agccatcccg catatcaaac gtctgcggat tcacagccgt
    16741 ctgccgattg tgatcccggc acgcatcaca gatgcgctgg ttgaacgctt ttctcattct
    16801 acgctgcaaa tcttgctggt gaatcacatc aaccatgcca atgaggtaga tgaaacattc
    16861 cgtcaggcga tggctaagtt gcgccgggtg ggcgttactt tgctgaacca gagcgttctg
    16921 ttacgtggtg tgaacgataa cgcgcaaacg ctggcaaacc tgagtaatgc gttgttcgat
    16981 gcgggcgtga tgccgtatta cctgcatgtg ctcgataaag tacagggcgc ggcgcatttt
    17041 atggtgagtg atgacgaagc acggcagatt atgcgtgagt tgctgacact ggtgtcggga
    17101 tatctggtgc ctaaactggc gcgagaaatt ggcggcgaac ccagcaaaac gccgctggat
    17161 ctccagctac gccagcagta actgattaaa cagggaagtt taatctttgc ccgttgcata
    17221 acctttaatt attcatatac attgctgttg ttactcttta tataaccaac accaataatt
    17281 aacctttctt ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat
    17341 tactaccaag cccatttatc tggcttataa actaaaatta attatttaat tgagaaatgg
    17401 ttagggagaa cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag
    17461 agtaataatt tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg
    17521 ttctttatgt ctgccatgga attgcgcgac cttctgattg cgctggaaag tcgtctgcat
    17581 caaaagcata aactggatgc tgctgcacgc cttcagtatg aacaagctcg cgataaagtc
    17641 attaagaaaa tggcagaaaa tatcccagaa attctggttg atgagcttaa aaatgccgat
    17701 attaatcgtc gggttaacac gctggaactt accgatgatc agggcgaaaa tcttaccttc
    17761 gtattaacgc tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa
    17821 atgctggcgc gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta
    17881 cgtattacct cactgttaga tttcctgccg ttatacgacg ttgactgcca ggaaaatggc
    17941 aatctggaat atgacaccta ttcccaaccg gaatggaaac ataacttgtt caaccattat
    18001 ctggcggtgc tttaccgctt taaagatgaa agtggcaatg aacaatttag cggcgcagtg
    18061 gtgaaaacgc gtgaagcaac gccgggtaaa gaagtggagg cgatcactcg ccgaatgctt
    18121 gatttcagcc cccgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc
    18181 gtagcggcaa ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac
    18241 ttacgtgttc aatccaccag caaaactgca ccacaggcga aataatctca tctgttttac
    18301 tacgaggctg gaagtatcca gcctctggat cacatcagtt cgggcactta taaacctgac
    18361 cgataatctg gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga
    18421 tgccataaat cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc
    18481 cgcgcataga accgccttct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg
    18541 tcgcagtacc aatcagctgg cactctgcac ctggttgctc gtccacaatg cgtacactct
    18601 gaccggcagc actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga
    18661 caatccctgc taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca
    18721 ctcgcgtcgt ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga
    18781 catttctgcc cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc
    18841 catgccaccc atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg
    18901 caggtcggta accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg
    18961 cagagcagaa cgggttactt tggttggatc caggataccc atatcgatca tgttgccgta
    19021 ttcttcagtt gctgcgttgt aaccgtagtt gccgtcgccg cctttaacgg tgttagcaac
    19081 aacagacggt tcttcgccgc agttcaggac gatctgacgc agcggagctt ccattgcacg
    19141 cagtgcaact ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt
    19201 agacgctacg cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc
    19261 acgggtcgcg tgcagggcgt cttcaacgcg tgcttttttc tctttcattt caacttcggt
    19321 agcagcacca actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag
    19381 tttttcacgg tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg
    19441 gccctggatt gcagcttctt cacccacgcc atcgatgatg gtggtggtgt ctttgttgat
    19501 cacaacacgt ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat
    19561 ctcttcagag attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg
    19621 acgatcgccg aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt
    19681 aacaaccaga gttgccagtg cttcgccttc aacatcttca gcgatgatca gcagcggttt
    19741 acctgctttg gcaacagctt ccagaaccgg cagcatttcg cggatgttgg agattttctt
    19801 gtcagccaac aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat
    19861 gaagtaagga gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc
    19921 ctgcagaccg gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc
    19981 ttcagcgatc agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc
    20041 aatcgcttta gagtcggagc atggtacgga cagtgttttc agttcttcaa ccgcagcagc
    20101 gacagctttg tcgataccac gtttcagatc catcgggttc atacccgcag caacagcttt
    20161 cagaccctca gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc
    20221 agcgtcgttc gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt
    20281 gtcttccagt tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc
    20341 gaaagattta tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc
    20401 tgccagtacg tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc
    20461 tttagctgcc attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gcggattacg
    20521 cttcaacaat tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct
    20581 tctcagattt cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca
    20641 gcggcttcac ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc
    20701 gggtggattt agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa
    20761 cttctttacg cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc
    20821 ctttgagaaa gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg
    20881 tgaccagaga aatggggatg aggcttcgcc ccttcaaggg ggaaaaagaa aaaaaattct
    20941 gatcagcaca aaatcgggtg aaaaccctga ttcacctcac atttcatcgc tatttcttca
    21001 tcaccgtcat aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat
    21061 ttgggcgcaa tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag
    21121 ggcatcggcc tgctatcgac gtcattatta ggcactggcg tgtttgccgt tcctgcgtta
    21181 gctgcgctag tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg
    21241 ttcccgattg cgattgtgtt tgcgattctg ggtcgccact atcccagcgc aggcggcgtc
    21301 gcacacttcg tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt
    21361 ttatcggtca ttcccgtggg tttgcctgcc gcgctacaaa ttgctgccgg attcggccag
    21421 gcaatgtttg gctggcatag cgggcaactg ttgttggcag aactcggtac gctggcgctg
    21481 gtgtggtata tcggtactcg aggtgccagt tccagtgcta atctacaaac agttattgcc
    21541 gggcttatcg tcgcactgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat
    21601 atccccttcc ctgcgccagg aaatatcgaa cttaccgggt tattcgctgc gttatcagtg
    21661 atgttctggt gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat
    21721 ccagagcgtg attttcctcg tgctttgatg attggcctgc tgctggcagg attagtctat
    21781 tggggctgta cggtagtcgt cttacacttc gacgcctatg gtgaacaaat ggcggcggca
    21841 gcatcgcttc ccaaaattgt agtgcagttg ttcggtgtag gagcgttatg gattgcctgc
    21901 gtaattggct atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc
    21961 ctggtctggt cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc
    22021 catatcccga ataatgccct caatgcagtg ctcggttgct gcgtggtgag cacattggtg
    22081 attcatgctt tagagctcaa tctggacgct cttattattt atgccaatgg catctttatt
    22141 atgatttatc tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta
    22201 ctggcggtgg ttggcgggct attatgcgtt ctgttactgg caatggtcgg ctggaaaagt
    22261 ctctacgcgc tgatcatgct ggcggggtta tggctgtttc tgccaaaacg aaaaacgccg
    22321 gaaaatggca taaccacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg
    22381 atcaaggcgg tcgcggtcat catcctttcg ctggtactca ccatcaaaag tattaccgcc
    22441 accggtcccg gcgctaaaac cgccgcctgg catacgggaa aaacgcagat gaggcatcaa
    22501 cttcactgtc agatgctttt gcaccggcgg taataaaaga agcaaaccga ggaaatcggt
    22561 gaaaaagccc ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat
    22621 ctccgccgct gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa
    22681 gccctggtta cgtaccagtg acataccgat aactgacgtg aatataacca gcacgagggt
    22741 cagcaatacc cccaatacat gagcaacctg aataaagatt gaaatctcaa tatagacata
    22801 aaggaaaatg gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggtt
    22861 aaacccggct acctgaatgg gttgcgaatc gcgtttagct tatattgtgg tcattagcaa
    22921 aatttcaaga tgtttgcgca actatttttg gtagtaatcc caaagcggtg atccatttca
    22981 caaattaata attaaggggt aaaaaccgac acttaaagtg atccaggtta cggtagaaat
    23041 cctcaagcag catatgatct cgggtattcg gtcgatgcag gggataatcg tcggccgaaa
    23101 aacattcgaa accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag
    23161 aaggttcaca tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt
    23221 ccagctgatg cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc
    23281 aacaacaaaa tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc
    23341 gcagctatgg caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt
    23401 gccgcatgtg atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc
    23461 taccagggcg gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc
    23521 ggtctggaac tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat
    23581 gttaacaaat gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac
    23641 tcttccctga ttaaactggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa
    23701 gctgtcgaat tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg
    23761 atgaccctcg gtcaggaatt ccgcgccttc agcatcttgc tgaaagaaga agtgaaaaat
    23821 atccaacgta ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact
    23881 ggtctgaaca cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact
    23941 ggcttcccat gcgtaccggc tgaagacctg atcgaagcga cctccgactg cggcgcttat
    24001 gttatggttc acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac
    24061 ctgcgcttgc tctcttctgg tccacgtgcc ggcctgaacg agatcaacct gccggaactg
    24121 caggcaggct cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac
    24181 caggtatgct tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt
    24241 cagctgcagt tgaacgttat ggagccagtc attggccagg ccatgttcga atccgttcac
    24301 attctgacca acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac
    24361 aaagaagtgt gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg
    24421 ttcatcggtc accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt
    24481 gtacgtgaag tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc
    24541 tccgtacaga atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa
    24601 cagtaatcgt acagggtagt acaaataaag aaggcacgtc agatggcgtg ccttttttct
    24661 tgtgagcagt aacttaaaaa taacaattta atatcaactt gttaaaaaac aaggaaggct
    24721 aatatgctag ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg
    24781 gggggaatag gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc
    24841 gttaaacccg gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct
    24901 atttctgcca tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg
    24961 ctgcgccgta acccgaaata catcacgatc ctcgcaccga tcgtgaccta tttcctgaca
    25021 atctttgctg gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg
    25081 aaggaacaag gcgttaaacc ttgccgtccg ctgtctactg cagtggtatc cgcgcagatt
    25141 gcgatcaccg catcgccaat ctcagcggca gtggtttaca tgtcttccgt aatggaaggt
    25201 catggcatca gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt
    25261 ctggtgatgt ccttcctggt cactatgctg ttcaactcca aactctctga cgatccgatt
    25321 tatcgcaagc gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc
    25381 aaatccggtg caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc
    25441 tatgcaatca tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc
    25501 aacgcaatcc tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc
    25561 gataccgaca acattctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt
    25621 atcctgggtg ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa
    25681 gataccgctg gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt
    25741 gcttctgctc tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg
    25801 gcactgaacg tttctccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc
    25861 attctgccga cctacccgac actggttgct gcggtacaga tggatgacac gggtactacc
    25921 cgtatcggta aattcgtctt caaccatccg ttcttcatcc cgggtactct gggcgttgcc
    25981 ctggccgttt gcttcggctt cgtgctgggt agcttcatgc tgtaatgacc catcgcgggg
    26041 cgttcacgcc ccgctttctt tcccgccgac taacatcctt tccccgtccg ttgtatagtg
    26101 acctctctct tgcagttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc
    26161 gaataccacg tctgtcgtgg tgctatgtac ggcaccggat gaagcgacag cccaggattt
    26221 agccgccaaa gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac
    26281 ctctctctat tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa
    26341 aactaccgta tctcaccagc aggcgctgct ggaatgcctg aagtctcatc atccatatca
    26401 aaccccggaa cttctggttt tacctgttac acacggagac acagattacc tctcatggct
    26461 caacgcatct ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc
    26521 gacgcgccgg gacgttcaca atttgtcccc gcggatcaag cctttgcttt tgattttcag
    26581 caaaatcaac atgatctgaa tctgacctgg cagatcaaag acggttacta cctctaccgt
    26641 aaacagatcc gcattacgcc ggaacacacg aaaattgctg acgtgcagct gccgcaaggc
    26701 gtctggcatg aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt
    26761 cccgtaacca tcaaccaggc gagtgcggga gcaacgttaa ctgtcaccta ccagggctgt
    26821 gctgatgccg gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc
    26881 gctaacaacg aagcgtcaca gcctgtgtct gttccgcagc aagagcagcc caccgcgcaa
    26941 ttgccctttt ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc
    27001 gtgctgccaa tgtacccact gatttctggc atcgtgctgg gcggtaaaca gcggctttcc
    27061 actgccagag cattgttgct gacctttatt tatgtgcagg ggatggcgct gacttacacg
    27121 gcgctgggtc tggtggttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca
    27181 tacgtgctca ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg
    27241 tttactctgc aactcccctc ttcgctgcaa acacgtctca cgctgatgag caatcgccaa
    27301 cagggcggct cacctggcgg tgtgtttatt atgggggcga ttgccggact gatctgttca
    27361 ccatgcacca ctgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg
    27421 tggctgggcg gcggcacgct ttatctttat gcgctgggca tgggcctgcc gctgatgcta
    27481 attaccgtct ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa
    27541 accgcgtttg gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt
    27601 gatatatggg gattacgctt gtggtcggcg cttggtgtcg cattctttgg ctgggccttt
    27661 atcaccagcc tacaggccaa acgcggctgg atgcgcgtgg tgcaaataat cctgctggca
    27721 gcggcattgg ttagcgtgcg cccacttcag gattgggcat ttggtgaaac acataccgcg
    27781 caaactcaga cgcatctcaa ctttacacaa atcaaaacag tagatgagtt aaatcaggcg
    27841 ctcgttgaag ccaaaggcaa accagtgatg ttagatctct atgccgactg gtgcgtcgcc
    27901 tgtaaagagt ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagat
    27961 acggtcttac ttcaggccaa cgtcactgcc aacgacgcac aagatgtggc gctgttaaag
    28021 catcttaatg tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat
    28081 ccacaagcac gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat
    28141 cgccaaccgt gaacgacact tgcagtggga aagacggagg aaaatagcgt gcaacgtgaa
    28201 gatgtactgg gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg
    28261 gagatggttg ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac
    28321 aaagaggcaa tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt
    28381 cgtcagctga tgctggacga aacgcaaacc gccgaacaaa agttgctggc acgttatcag
    28441 gcgttatcgg agtgcgttaa aaacaaccgc tatccgggct gtctgtttat cgctgcctgt
    28501 acgttttacc ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg
    28561 gcctacgatt tcacccacga actgttaacc acgctggaag ttgacgatcc ggcgatggta
    28621 gcaaagcaga tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc
    28681 caggcggatg tcgacaccgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc
    28741 cgtcagggtg gtgcactgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt
    28801 aagcagttga atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat
    28861 gcgccccgtt gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct
    28921 tggttcgatt ccgagtccgg gcaccaaatt catatcaacg gacctccacg gaggtccgtt
    28981 tttcattcca ggacgccacg atttaagcgt cctgcctcca aatcaattct accgaactca
    29041 accagattcc ccccacatca acctcattgt gtgggtataa ttgtgggcat accccagttc
    29101 gacagaattt gtaccctctt taacgca
//
LOCUS       DADSZD010000042        28793 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.042, whole genome shotgun sequence.
ACCESSION   DADSZD010000042 DADSZD010000000
VERSION     DADSZD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28793
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.042"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(72..1196)
                     /locus_tag="J1Z28_001668"
     CDS             complement(72..1196)
                     /locus_tag="J1Z28_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7716509.1"
                     /translation="MSTLFTINACKTFGCRNLGLASSEDYSWPDYKLGYPALHCRACG
                     SYPPLFDEQQFRDWLSVHLSTYAIEKGHFCPVCYGTDMICYGHNPQGSQRIQCRNCKK
                     VWTPKKYQKEITHPQAIETVQLFIPFQGASAVQKLYVLVSLDATRGNILHLSTNYTQH
                     QTGDSLRYSYKGNTEPTMHHHDIVQKVDMREAQFLRRSQFDEIQYGSAVLKRNGKGAI
                     LRPVITAHGHFRILKIRYPDVKTHIISHECFLRGAIITAWADQFRQQQGELWFVEEEI
                     SDSNADTPWHFKGTTYHGWWQNQWQRWEQGNNCKMVCLLTGACLERGANVSLATSRCF
                     ITWLTDQHDFTQSALLSAGRVTQMLTSLALKYNESLTPSC"
     gene            1329..2882
                     /locus_tag="J1Z28_001669"
     CDS             1329..2882
                     /locus_tag="J1Z28_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7716510.1"
                     /translation="MKIKILAAGIALTLPFWACAKDVTIIYTNDLHAHVEPYKVPWIA
                     DGKRDIGGWANITTLVKQEKAKNKATWFFDAGDYFTGPYISSLTKGKAIIDIMNTMPF
                     DAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIG
                     VIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQS
                     SMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
                     KLVLDYKEKPHNFTVKNFELKTIYADEWKPDQQTKQVIDGWNKKLDEVVQQTVAQSPV
                     ELKRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPF
                     PNELVTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKP
                     IEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQL
                     NGMRVKDIK"
     gene            3264..4154
                     /gene="metF"
                     /locus_tag="J1Z28_001670"
     CDS             3264..4154
                     /gene="metF"
                     /locus_tag="J1Z28_001670"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBA7716511.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMVQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            4483..6663
                     /gene="katG"
                     /locus_tag="J1Z28_001671"
     CDS             4483..6663
                     /gene="katG"
                     /locus_tag="J1Z28_001671"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBA7716512.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASATGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            6757..7662
                     /gene="yijE"
                     /locus_tag="J1Z28_001672"
     CDS             6757..7662
                     /gene="yijE"
                     /locus_tag="J1Z28_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBA7716513.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(7689..8306)
                     /locus_tag="J1Z28_001673"
     CDS             complement(7689..8306)
                     /locus_tag="J1Z28_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBA7716514.1"
                     /translation="MKASLALFSLLTVFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(8582..9685)
                     /gene="gldA"
                     /locus_tag="J1Z28_001674"
     CDS             complement(8582..9685)
                     /gene="gldA"
                     /locus_tag="J1Z28_001674"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBA7716515.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(9696..10358)
                     /gene="fsa"
                     /locus_tag="J1Z28_001675"
     CDS             complement(9696..10358)
                     /gene="fsa"
                     /locus_tag="J1Z28_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA7716516.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(10370..12871)
                     /gene="ptsP"
                     /locus_tag="J1Z28_001676"
     CDS             complement(10370..12871)
                     /gene="ptsP"
                     /locus_tag="J1Z28_001676"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7716517.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAYQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHHQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            13180..14259
                     /locus_tag="J1Z28_001677"
     CDS             13180..14259
                     /locus_tag="J1Z28_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBA7716518.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICVPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            14274..14594
                     /locus_tag="J1Z28_001678"
     CDS             14274..14594
                     /locus_tag="J1Z28_001678"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBA7716519.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            14645..16942
                     /locus_tag="J1Z28_001679"
     CDS             14645..16942
                     /locus_tag="J1Z28_001679"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA7716520.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            16908..17786
                     /locus_tag="J1Z28_001680"
     CDS             16908..17786
                     /locus_tag="J1Z28_001680"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBA7716521.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIKQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            17788..18129
                     /locus_tag="J1Z28_001681"
     CDS             17788..18129
                     /locus_tag="J1Z28_001681"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBA7716522.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(18116..18967)
                     /locus_tag="J1Z28_001682"
     CDS             complement(18116..18967)
                     /locus_tag="J1Z28_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7716523.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(19193..20926)
                     /gene="cptA"
                     /locus_tag="J1Z28_001683"
     CDS             complement(19193..20926)
                     /gene="cptA"
                     /locus_tag="J1Z28_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBA7716524.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(21109..23760)
                     /gene="ppc"
                     /locus_tag="J1Z28_001684"
     CDS             complement(21109..23760)
                     /gene="ppc"
                     /locus_tag="J1Z28_001684"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBA7716525.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(24062..25213)
                     /gene="argE"
                     /locus_tag="J1Z28_001685"
     CDS             complement(24062..25213)
                     /gene="argE"
                     /locus_tag="J1Z28_001685"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBA7716526.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            25367..26371
                     /gene="argC"
                     /locus_tag="J1Z28_001686"
     CDS             25367..26371
                     /gene="argC"
                     /locus_tag="J1Z28_001686"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBA7716527.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            26379..27155
                     /gene="argB"
                     /locus_tag="J1Z28_001687"
     CDS             26379..27155
                     /gene="argB"
                     /locus_tag="J1Z28_001687"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBA7716528.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            27216..28589
                     /gene="argH"
                     /locus_tag="J1Z28_001688"
     CDS             27216..28589
                     /gene="argH"
                     /locus_tag="J1Z28_001688"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBA7716529.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
BASE COUNT         6686 a         7215 c         7889 g         7003 t
ORIGIN      
        1 acatttatat atttaattaa atgaaaggta gtgatctctc ttaatttatg gtcgtttgtc
       61 gagtgtattg actaacaaga tggagtgagc gattcattgt attttaacgc cagtgaggtc
      121 agcatttgcg ttacgcgacc tgcggataat aacgcgcttt gggtaaagtc gtgttggtct
      181 gtcagccatg tgataaaaca acgactggtt gccagagaaa catttgcgcc cctttctaag
      241 caggctcccg tgagtaggca gaccatcttg cagttattcc cctgctccca gcgctgccac
      301 tgattttgcc accaaccatg gtatgtcgtt cccttaaaat gccagggcgt atcagcgtta
      361 ctgtcactga tttcttcttc tacgaaccaa agttcacctt gttgttggcg gaactgatct
      421 gcccaggctg taataattgc gcctcttaga aaacattcgt gtgaaataat atgcgttttg
      481 acatctggat agcggatttt cagtattctg aaatgcccgt gtgccgtgat aaccgggcgt
      541 agtatggctc ccttaccgtt gcgcttgagt acagcactgc catactgaat ttcatcgaac
      601 tggctccggc gcaagaattg cgcttcacgc atatctacct tctgcacgat atcgtgatgg
      661 tgcatagttg gttctgtgtt gcccttgtaa ctgtaccgca gactgtctcc tgtctgatgt
      721 tgcgtataat tcgtggagag atgaagaatg ttgccacgag tggcgtcaag actgactaaa
      781 acatacagtt tttgtactgc gctggcccct tgaaagggta tgaacagttg aacggtttca
      841 atggcctggg gatgcgtaat ttctttctga tattttttag gcgtccagac ttttttgcag
      901 ttacggcatt gaatgcgttg cgaaccctgt ggattgtgac cataacaaat catgtctgtg
      961 ccataacata ctgggcagaa atggcctttc tcaattgcat aagtagacaa gtgaactgac
     1021 aaccagtcac gaaattgttg ctcgtcaaac aacggcgggt aactgccaca tgcccgacag
     1081 tgcagtgccg gataacccag tttatagtct ggccaactgt aatcttccga tgaggccaat
     1141 cccaaatttc ggcaaccgaa cgttttacag gcattaatcg taaacagagt cgacatatat
     1201 caccaacgat ttcacaaggt cgttgatttt gtccggcaac tatcccctgc ggatgtgatt
     1261 actctcacat aaataagtct ttcagaacgt aatgtagcaa tcctttatct cactgaaagg
     1321 gattgtttat gaaaattaaa atacttgctg cgggtatcgc attaacactg ccgttctggg
     1381 cttgcgctaa agatgtcact attatttata ccaacgacct ccatgcgcat gtagaacctt
     1441 ataaagtgcc gtggatcgct gacggtaaac gggatattgg cggctgggca aatatcacta
     1501 cgctggttaa gcaagaaaaa gcaaaaaata aagcgacatg gttttttgat gcgggcgact
     1561 attttaccgg gccatatatc agcagcctga ccaaaggtaa ggctattatc gatattatga
     1621 atactatgcc gtttgatgcc gtcacaatag gcaaccatga atttgaccac ggctgggaca
     1681 acacattatt acagttgagc caggcaaaat tccctattgt gcagggcaat attttttatc
     1741 agaacagcag taaatcattc tgggataagc cctataccat catcgaaaaa gacggtgtga
     1801 aaataggcgt tattggtttg cacggagtat ttgcctttaa tgatacggta tctgctgcca
     1861 cgcgcgtggg tattgaagcg cgcgatgaaa ttaaatggct acaacgttat atcgatgaac
     1921 tcaaaggcaa ggttgatttg accgtcgccc tgatccacga aggtgttcca gcccgccagt
     1981 ccagtatggg aggcacagat gtgcgtcgcg cgttggataa agatattcag acggcaagtc
     2041 aggtgaaagg gctggatatt ttgatcaccg gacatgcaca tgtgggtacg ccggaaccga
     2101 ttaaagtcgg caatacgctc atcctctcca ctgacagcgg cgggattgat gtgggcaaac
     2161 tggttctcga ctacaaagag aagccgcata attttacggt gaaaaacttc gagcttaaaa
     2221 ccatttacgc tgacgagtgg aagcccgatc agcaaacgaa acaggtgatc gacggctgga
     2281 ataaaaagct tgatgaagtg gtgcaacaaa cggtggcgca atcgccggtt gaattaaaac
     2341 gtgcctatgg tgaatcggct tccctcggga acctggcggc tgacgcttta ctggcagcag
     2401 cgggtaaaaa tacccaactg gctttaacaa actctggtgg tatccgcaat gagatccccg
     2461 caggggcgat cacgatgggc ggcgtcatca gtaccttccc gttccccaac gaactggtga
     2521 cgatggaact cacgggtaaa caattacgca gtctgatgga acacggcgca agtttaagca
     2581 atggcgtttt acaggtatcg aaaggcctgg aaatgaagta cgacagcagc aagccggttg
     2641 gtcagcgggt aatcacactg actctgaatg gcaaacccat tgaagatgcg acggtttatc
     2701 acattgctac tcagagtttc cttgctgatg gtggagatgg ttttaccgcc tttaccgaag
     2761 ggaaagcgcg taacacaacg ggcggttatt acgtttatca cgcggtggtt gattacttca
     2821 aagcgggcaa caccattacg gatgaacagc tcaacggtat gcgcgtgaag gatatcaaat
     2881 aaaagtgcgc actgaattga ctcaactgtt tgttacccgt tgattgcgca gatcctgtat
     2941 tcggcctacg cgagggcgca ggccgaatca gatgccttaa catcaccatc cggtggcagg
     3001 acgttgtttt tcgaattagc ccaggtgtcg ctgtgttgtc agaatatgag tcaaaacgtg
     3061 cgaggtggat tgcgctatag agaaatcttt tatcgcctct aattaagatg agtttttttc
     3121 atcttccctg atttttcctc accatcattg gttatttttc ggttgacgcc cttcggcttt
     3181 tccttcatct ttacatctgg acgtctaaac ggatagatgt gcacaacaca acatataacg
     3241 acaagcgatt gatgaggtaa ggtatgagct tttttcacgc cagccagcgg gatgccctga
     3301 atcagagtct ggcagaagtc caggggcaga ttaacgtttc gttcgaattt ttcccgccgc
     3361 gtaccagtga aatggagcag accctgtgga actccatcga tcgccttagc agcctgaaac
     3421 cgaagtttgt atcggtgacc tatggcgcaa actccggcga gcgcgaccgt acgcacagca
     3481 ttattaaagg cattaaagat cgtaccggcc tggaagcggc accgcatctt acctgtattg
     3541 atgcgacgcc tgccgagctg cggaccattg cacgcgacta ctggaataac ggtattcgcc
     3601 atatcgtggc gctgcgtggc gacctgccgc cgggaagtgg taagccagaa atgtatgctt
     3661 ctgacctggt gacactgtta aaagaagtgg cagatttcga tatctccgtg gcggcgtatc
     3721 cggaagtaca tccagaagcg aaaagtgccc aggccgattt actcaacctg agacgaaaag
     3781 tggatgccgg agccaaccgc gccattactc agttcttctt cgatgtcgaa agctacctgc
     3841 gttttcgcga tcgctgcgtc tcggcaggca ttgatgtgga aatcattccg ggaattttgc
     3901 cggtttcgaa ctttaaacag gcgaagaaat ttgccgacat gaccaacgtg cgtattccgg
     3961 cgtggatggt acaaatgttc gacggcctgg atgacgatgc tgaaacccgc aagctggtgg
     4021 gcgcgaatat tgccatggat atggtgaaga ttttaagccg tgaaggagtg aaagatttcc
     4081 acttctatac gcttaaccgc gctgaaatga gttacgcgat ttgccataca ctgggagttc
     4141 gacctggttt ataaatagtg tgccttttgt gaaaatcaca cagtgatcac aaattttaaa
     4201 cagagcacaa aatgctgcct cgaaatgagg gcgggaaaat aaggttatca gccttgtttt
     4261 ctccctcatt acttgaagga tacaaatcta aaaccctttt ttataaagca tttatcctaa
     4321 atcggacata atcaaaaagt cttaattacg atcaatttga tctacatctc tttaaccaac
     4381 aatatgtaag atctcaacta tcgcatccgt ggattaattc aattataact tctctctaac
     4441 gctgtgtatc gtaacggtaa cactgtagag gggagcacat tgatgagcac gtcagacgat
     4501 atccataaca cgacagccac cggcaaatgc ccgttccatc agggcggtca cgaccagagc
     4561 gcaggtgggg gcacaaccac gcgcgactgg tggccaaatc aactccgtgt tgacctgtta
     4621 aaccaacatt ctaatcgttc taacccgctg ggtgaggact ttgactaccg caaagaattc
     4681 agcaaattag attactacgg cctgaaaaaa gatctgaaag ccctgctgac agaatctcaa
     4741 ccgtggtggc cagccgactg gggcagctac gccggtctgt ttattcgtat ggcctggcac
     4801 ggcgcgggta cttaccgttc aatcgacggt cgcggcggcg cgggtcgtgg tcagcaacgt
     4861 tttgcaccgc tgaactcctg gccggataac gtaagcctcg ataaagcgcg tcgcctgttg
     4921 tggccaatca aacagaaata tggtcagaaa atctcctggg ccgacctgtt tatcctcgcg
     4981 ggtaacgtgg cgctggaaaa ctccggcttc cgtaccttcg gttttggtgc cggtcgtgaa
     5041 gacgtctggg aaccggatct ggatgttaac tggggtgatg aaaaagcctg gctgactcac
     5101 cgtcatccgg aagcgctggc gaaagcaccg ctgggtgcaa ccgagatggg cctgatttac
     5161 gttaacccgg aaggccctga tcacagcggc gaaccacttt cggcagcagc agctatccgc
     5221 gcgaccttcg gcaacatggg catgaatgac gaagaaaccg tggcgctgat tgcgggtggt
     5281 catacgctgg gtaaaaccca cggtgccggt ccgacatcaa acgtaggtcc tgatccagaa
     5341 gcagcaccga ttgaagaaca aggtttaggc tgggcgagca cttacggcag cggcgttggc
     5401 gcagatgcca ttacctctgg tctggaagtg gtctggaccc agacgccgac ccagtggagc
     5461 aactatttct tcgagaactt gttcaagtat gagtgggtac agacccgcag cccggctggc
     5521 gcaatccagt ttgaagcggt agacgcaccg gaaattatcc cggatccgtt tgatccgtcg
     5581 aagaaacgta aaccgacgat gctggtgacc gacctgacgc tgcgttttga tcctgagttc
     5641 gagaagatct ctcgtcgttt cctcaacgat ccgcaggcgt tcaacgaagc ctttgctcgt
     5701 gcctggttca aactgacgca cagggatatg gggccgaaat ctcgttacat cggaccggaa
     5761 gtaccgaaag aagatctgat ctggcaagat ccgctgccgc agccgatcta caacccgacc
     5821 gagcaggaca ttatcgatct gaaattcgcg attgcggatt ctggtctgtc tgttagtgag
     5881 ctggtatcgg tggcctgggc gtctgcttct accttccgtg gtggcgacaa acgtggtggt
     5941 gccaacggtg cgcgtctggc attaatgccg cagcgtgact gggatgtgaa cgccgcagcc
     6001 gttcgtgctc tgccagttct ggagaaaatt cagaaagagt ctggcaaagc ttcgctggcg
     6061 gatatcatcg tgctggcagg tgtggttggc gttgagaaag ccgcaagcgc cacaggtttg
     6121 agcattcatg taccgtttgc gccgggccgc gttgatgcgc gtcaggatca gactgacatt
     6181 gaaatgtttg agctgctgga gccaattgct gacggtttcc gtaactaccg cgctcgtctg
     6241 gacgtttcca ccaccgagtc attgctgatt gataaagccc agcaactgac gttgaccgcg
     6301 ccggaaatga ctgcgctggt gggcgggatg cgcgtactgg gtgccaactt cgatggcagc
     6361 aaaaacggtg tcttcactga ccgcgttggt gtattgagca atgacttctt cgtgaacttg
     6421 ctggatatgc gttacgagtg gaaagcgacc gacgaatcga aagaactgtt cgaaggccgt
     6481 gaccgcgaaa ctggcgaagt gaaatacaca gccagccgtg cggatctggt ctttggttct
     6541 aactctgtcc tgcgtgcggt ggcggaagtt tacgccagca gcgatgccca cgagaagttt
     6601 gttaaagact tcgtggcagc gtgggtgaaa gtgatgaacc tcgaccgttt cgacctgctg
     6661 taatctgacc ttcttcagcg actgcctttc atgcagtcgc tgaagtttct ttaccggcgt
     6721 atagtgtcca caggaaaact acacactgga tctctcatgt ctgccgcagg aaagagcaac
     6781 ccactggcaa tcagtggcct ggttgtgctc acacttatct ggagttatag ctggattttc
     6841 atgaagcaag tcaccagcta catcggtgcc ttcgacttca ccgccttacg ctgcattttc
     6901 ggcgctctcg ttttattcat cgttctttta ttacgtggtc gcggaatgcg cccgacgccg
     6961 tttaaataca ccttagccat tgccctgtta caaacctgcg ggatggttgg cctggcacag
     7021 tgggcgctgg tcagcggcgg agcggggaag gtggcaatcc tgagctatac catgccgttc
     7081 tgggtggtga ttttcgccgc gttgtttctc ggtgaacgcc tgcgacgtgg gcaatatttc
     7141 gcgatactga ttgccgcttt cggcttattt ttggtgttgc agccgtggca actcgatttc
     7201 tcttcgatga aaagtgccat gctggcaatc ctctccggtg tgagttgggg ggcgagcgcg
     7261 attgttgcta aacgtctgta tgcccgccat ccgcgcgtgg atttattgtc gctaacatcc
     7321 tggcagatgc tgtatgcggc gctggtgatg agcgtggtcg ctttactggt gccgcaacgt
     7381 gaaattgact ggcaacccac cgtgttctgg gcgctggcct acagtgcgat tctggcgaca
     7441 gcgctggcgt ggagcttatg gttgtttgta ttgaaaaact tgcctgccag tattgccagc
     7501 ttaagcacac tggccgttcc cgtttgcggc gtactctttt cctggtggct gctcggtgag
     7561 aatccggggg cagttgaagg tagcggtatt gtgctgattg tactggcact ggcgctggtg
     7621 agccgtaaga aaaaagaagc cgtcagtgta aaaaggatct gaattttttc ttcatgtggg
     7681 gagatctctt atttagcaaa ataccgatag tgccccacca tccgccagct aaacagtaca
     7741 tcttcttcct gcgcgcctgc gccaatgtta tgtatcacca gcggcgtacc gtcgcgggcg
     7801 aagccatctg acaccacccc aatatgtgcc agcccgttat ctaatcgcca ggagacaata
     7861 tcgcccgctt gatagtcact ggggttcttg ctggtggggc gtgttttatc gtggcgggta
     7921 aaccaggttt ccagattagg cacccgacgg tgatcgatgt tgctgtccgg gcgctttaac
     7981 tgccattttt gcgggtacgc agcaaaattc ttcgccatat cttcgtgcac cagtttctgc
     8041 aaatcgacct tctggctgcg caatgcacgg atcaccacat cggaacatac gccgcgttct
     8101 tgcgggacat caccgccagg ataggtaagc tgcacatacg ccggatcgta aaatagcgtg
     8161 ctgccgattt gctgtctggc gccgtctgcg atggcaagat tggtgttagc ctggatttgt
     8221 accacggttg gtggaacggc gggagatttt aaggagtaac tggtaaatac cgtcagaaga
     8281 ctgaataacg ccagtgaagc tttcatctgt ttcgtccgtg aaaataagag tcacagattc
     8341 gaccttccgg gctaacgatt gttctgtgga gaaaatgtcc agaagcggat aaaaaaacac
     8401 ccgaacaagg tggaagtttc agattactgg cacagcgtgc cggatgcggc gtgaacgcct
     8461 tatccggcct acaaaagtac gtaaatttac tatattgcag agattgcgta ggcctgataa
     8521 gcgtagcgca tcaggcaatt ttgcgttcaa actcccggac aagccgggag tttggagtag
     8581 gttattccca ctcttgcagg aaacgctgac catactggtc ggctaccagc agtgcggcgt
     8641 aaacctgatc tggcgtcgcg ccgccaggca tgttgtggat ggtttcacct tctgcacatg
     8701 ccgcttctgc cacaattcgc attttcgccg ggacatcttc tttaatatcc agttgagcga
     8761 gagttattgg caaacctacc gcatggctaa gcgcagctac ggtttcgatt tcctccaccg
     8821 gcgcgttttc cagaaccagc tgcgtcagcg taccgaatgc tactttttca ccatgataat
     8881 agtgatgcgc gtccgggata gcggtcaggc cgttatgtac cgcgtgtgcc gccgccagcc
     8941 cgccgctttc aaaaccaacg ccactcaaat aggtgttcgc ttcaatcacg cgctccagcg
     9001 ccggagtcac tacatgctgt tcggcggcaa gcatcgcttt ttcgccttct tccagcaggg
     9061 tgttgtagca cagttcagcc agtgccagcg ctgcctgagt gcacttgcca cccgccatgg
     9121 tggtcgcgcc gctacgggaa caggcacgcg cttcaaacca ggttgccagc gcatcgccga
     9181 tacccgccgc taacagacgt gcaggtgcgc cagcaacgat tttggtgtcg acaatgacca
     9241 tattcgggtt atttggcaac agcagatagc ggtcaaactc accctcatcg gtgtagataa
     9301 cagacaatgc gctgcacggt gcgtcggtgg aagcgatagt cggtgcgatc gctaccggaa
     9361 cacccatgaa atgtgccagt gctttggcag tatcgagggt ttttccgcca ccgataccga
     9421 gaattgcgcc acactgcgca gtctccgcga tgccacgcag acggtcgatc tcattttgcg
     9481 aacattcacc gccaaacggc gcaatttcta ctaccagtcc agcatcttta aagcttttct
     9541 cgacagtgga ttgagcaaaa cctaaaacaa atttgtcacc caccactaac cagcgttccg
     9601 ccagtggctt caggtactcg cccagacgat taattacatc tgcgccctgg atgtatttac
     9661 ctggtgattg aataatgcgg tccataatta ctcctttaga gatgagtagt gccaaatgcg
     9721 gcattccagt cgtgctcgaa cttctctata gctgactcta ccgcaggggt gttaagcatt
     9781 tgttgcgcta catctaaggg cagtgtgatg gattcacatc cagccagcaa acaatccagc
     9841 gcctgacgtg gtgttttaaa gctggcagcc agcaccatgc tttctggcgc atgcatttcc
     9901 aacagcgctt gcagctcctg tacagtacga atgccgtcac cgccctgggc atctacgcgg
     9961 ttaacgtatg gagcaacgta tttggctcca gccagcgccg ccagtaatcc ttgcgcggcg
    10021 ctgtacactg cggttcccag cgtagtaatg ccttctttct tcagcatttt aattgctgcc
    10081 agaccttcag aggttaccgg aattttcacc acaatgcccg gaatagcgtc gcgcaggcgt
    10141 ttcgcttctt tcaccatacc ctgcgcgtcg cggctcatgg tctgagcaaa cagaatgccc
    10201 tcatcaccga tcgctttttg aaggcgcggc agcacttccc agatggactc cttgctggca
    10261 gcgataatgc tcgggttagt tgtcaccccg gcaatcggga atatgcgtgc cagacgttcg
    10321 acttctgcga cgttagcggt gtccagatac agttccatga tgtttccttt tacagttcca
    10381 gttcatgttg cagcaggctg gcgatagcgt cggcagaggc ggcgttaacc agcgcattac
    10441 ggaattcttc gtgcatgatg cgacgagcga ggcgcgagaa aatgcgcata tgttgatcgc
    10501 ccgcggcgtg tttgttcagg gttaacataa tgatgaattg cgcttcatca tcgccccagc
    10561 gcactggcgc ttgcagacgc gctacgctga tggtggattg ctcaatgtgt tccgatttac
    10621 tgtggggaat ggcgaaacta aagcccagtc cggtagagaa aacggcctcg cgcgcccaca
    10681 agtcggcttc cagtttacgc ggatagcggc agcggcccgc cagcagcagg ttatcggtca
    10741 tgcctttgag cacttcttct ttgctgcgcc agtcgctttc cagtgtgatg cactcggcgg
    10801 tgaccagcgg tgcgtcctgt tgggtcatgc ggaattgcgc cagcaggtgt tccacttcca
    10861 gcgaagtacg gcaggccatt gcctggttga gcaactggcg gcactcacgg ctatcaagtt
    10921 gcgccatccg cgctttcgcc gccggaattg atggtgcgct catgctgagt tcatccagcc
    10981 ctaaaccgac cagcaacggc agcacggaac ctttcgctcc cagttcaccg cacagaccaa
    11041 tccatttgcc ctggtgatgc accgcctgca cggcgtagtc gagcgcccgc aagaaggccg
    11101 gattcaggct gttgtagtga cgagtcacct tagcgttatc gcgatccact gccagtagat
    11161 attgcgtcag gtcattacta ccaatactaa agaaatcaat ctcttcgcag cattgatcga
    11221 tgatgaacat caccgacggc acttccagca tgatgccgag ctggatcttc tcatcaaacg
    11281 gaatgtgttc gttacgcagt tgctgtttgg cttccgccag tttttctttc acccataaga
    11341 tctcttccat tgaggagatc atcgggatca tgattttcag gctgccgtga gcagaggcgc
    11401 gcaggatcga ccgtagttgt gtggtaaaca acgacgcgta ttcttcataa atacgcaccg
    11461 cgcgataacc gaggaacggg ttagcttctg ccggaatgtt cagataatca acgggtttat
    11521 caccgccaat gtccatagtg cgcacaataa tgctgcgtcc gttggcagac tccagcgcct
    11581 gacaaaaaat gttatacagc tcgctttcac ccggtgcact ggtgcgatcc atatagagca
    11641 tttcagtgcg gaacaaacca acgccttccg caccattgcc gaatgcggcc tgcgcttcca
    11701 cggagtgagc gatgttagcg gcaatttcaa tgcggatacc gtctgcggta cgcgcttgct
    11761 gggtcagcca gacacgctgt tgctcacgca gggcgtcctg tacgcgggct tcctgctgat
    11821 aataacgagc tacggcttcc cctggctcaa ccacaatcgc cccagcgttg ccgtcgatat
    11881 aaatcgtctg atgctgccac ggagtaaggg catcaatatc cacacctacc agcgtcggaa
    11941 tgttgaacga acgggcaagg atcactgtat gtgatgtggt gccgccactt ttcagtaaca
    12001 gacctttgag atgattttta tccagttcga ggaactggct gggggtcagt tcatcagcca
    12061 tacaaatggc gggctgcgtc agtttgcccg gtgccgggaa gcgttgctca ccgtagattt
    12121 gctggagtaa ctggaagcag acgtcgcgta cgtccagggc acgttcttgc aggtagctgc
    12181 tgctggaacg ggcgaactct tcgcaaaagt gattcgcact ggcaacaatc gcttcggcgc
    12241 agcttaatcc ggcgctgaca cctgccagta aatgttcgcg cagggaagta tcgccagcca
    12301 gggatcggtg cgcttccaga atcgcgctgg tagcaccgtc gctatccagc agacgaaact
    12361 ctatgttttt cagtaccagc gtcagaccgt tttccagtgc ggattgttcg gcgtcaacgc
    12421 ctttggctgc gggaagatta cccagcgcat tgagatctaa agaagagatc ggcgtcagaa
    12481 tgccgcctgc gctaccgctg cacacggtgc gagcacggat aatttgcgga ttcagattgg
    12541 tcagtgaaat cggcagtggt tccagttcgt cagattttac ttccgccagc ggtgcgtcgc
    12601 agtgggggaa ttcatcgcgc agccattggc ttaaacgctg gtacgcttcc tgttcgtcgg
    12661 ccccggaaat cagtaactgg cagttatcgc ccaccagcgt atcggtgcca atcagcgcaa
    12721 gggcgctttt ggcgttgccc ttgcggtcag tgcgcaggtt atgccactca atttgtgatg
    12781 aaaaagtatt acacagcgtt tcaacgtggc ttgccggacg cgcatggacg ccgttaggta
    12841 gctcacaaat aaattccaca atcagggcca ttgcctttct cccggtacgg ttttctgttt
    12901 acaggataag agcgcacggt aacggcctgc catgtgacaa atctgccaaa agctggacaa
    12961 atgtaatgta accgtcaatt tgcgacgcgt ctcacaagac gccgttttgc ggcatgcttc
    13021 cggattatcg caagttatga ggcggatcgc atttttgtac tgatattaca aaagtccagt
    13081 aaatggcctt tttatccact gtttgctccg cctgcgattg cctattgttt cttccatcaa
    13141 aaatatggac atgggctttc gcaagcccgg gagcagctta tgaatgagtt ggtgcagatc
    13201 ctgaaaaata cccgtcagca tttaatgacg ggcgtttcac acatgattcc cttcgtggta
    13261 tcgggcggta ttttgctggc ggtttccgtc atgttgtatg gcaaaggcgc agtgccggat
    13321 gccgtagccg atccgaatct gaaaaaactg tttgatatcg gcgttgcagg tttgacgctg
    13381 atggtgcctt tcctcgccgc ttacatcggt tattccattg cagaacgttc tgcgctggct
    13441 ccatgcgcta tcggtgcctg ggttggtaac agctttggcg cgggcttctt tggtgcgctg
    13501 atcgccggga ttatcggcgg catcgtggtg cattacctga agaaaattcc ggtgcataaa
    13561 gttctgcgct cggtgatgcc aatcttcatc attccaatcg tcggcacact gattaccgct
    13621 ggcgtcatga tgtgggggct gggcgagccg gtgggggcgt tgaccaacag cctgacccag
    13681 tggcttcagg ggatgcagca gggcagcatt gttatgctgg cggtgatcat gggtctgatg
    13741 ctggcgttcg atatgggcgg cccggttaac aaagtggcct atgccttcat gctgatttgc
    13801 gttgctcagg gtgtttatac cgtggtggct attgccgctg ttgggatttg tgttccaccg
    13861 ctggggatgg ggctggcgac gctgattggt cgtaaaaatt tctccgcaga agagcgcgaa
    13921 accggcaagg cggcactggt gatgggctgt gttggcgtta ctgaaggggc gattcctttc
    13981 gccgctgccg atccgctgcg tgtcattcct tccatcatgg tcggttcagt ttgtggtgcg
    14041 gtaactgcgg cgctggtcgg tgcgcagtgc tatgcaggct ggggtggtct gattgtgctg
    14101 ccggtggttg aaggtaagtt gggttacatc gcggcagtgg ctgtcggggc ggttgtgacg
    14161 gcagtctgcg tgaacgtgct gaaaagtctg gcgcgtaaaa atgggtcttc aactgaagag
    14221 aaggaagacg acctggatct ggattttgaa attaattaat tgaggaacga gccatgacga
    14281 aaattatcgc agtaaccgca tgtccttcag gtgttgccca tacttatatg gcggcagagg
    14341 cgctggaaag cgccgcgaaa gcgaaaggct gggaagtgaa agtagaaacc caggggtcga
    14401 ttggtctgga aaatgaactg actgcggaag acgtggcaag cgccgatatg gtgattttga
    14461 ccaaagatat cggcatcaaa tttgaagagc gttttgctgg caagactatc gtgcgtgtca
    14521 acatcagcga cgcggtaaag cgtgctgacg ccattatgag caaaattgaa gctcacctgg
    14581 cacaaaccgc ttaagttctt atcccgcccg caagggcggg ttcgctttcc cacaggagtt
    14641 cctcatgacg aatcgtatct ctcgcctcaa aactgcactg tttgccaata cccgtgaaat
    14701 ctcgctggag cgggcgttgc tttataccgc cagccatcgg caaaccgaag gggagccggt
    14761 gataatgcgt cgggcgaaag caacagcgta tatccttgaa cacgttgaaa tttcgattcg
    14821 tgatgaagaa ctgattgccg gtaaccgcac cgtaaaacca cgcgccggga ttatgtcgcc
    14881 ggaaatggac ccttactggc tgctgaaaga gctggatcaa ttcccgacgc gtccgcagga
    14941 ccgctttgct atcagcgaag aagataaacg tatctaccgc gaagagttgt tcccgtactg
    15001 ggaaaaacgt tcgatgaaag atttcatcaa cgggcagatg acggatgaag taaaagccgc
    15061 gaccagcacg cagattttca gcatcaacca gacggataaa ggccaggggc acattattat
    15121 tgattacccg cgcctgctga atcacgggct gggggagctg gtggcacaga tgcagcaaca
    15181 ttgtcagcaa cagccggaga atcactttta tcaggcagcg ctgttactgc tggaagcctc
    15241 gcagaaacat attttgcgtt acgccgaact ggcggaaacg atggcggcaa gctgcactga
    15301 tggccagcgt cgcgaagaac tgctgaccat tgcggagatc tcccgccata acgccgaaca
    15361 taagccgcag acgttctggc aggcgtgcca gttattctgg tacatgaaca tcattctgca
    15421 atacgaatcc aacgccagtt cgctatcgtt ggggcgcttc gaccagtata tgttgccgtt
    15481 ctatcagaca tcattaaccc agggcgaaga tccggcattc ctgaaagaac tgctcgaatc
    15541 tttatgggtg aaatgcaacg acatcgtgct gttgcgctcc accagtagcg cgcgttattt
    15601 cgcaggtttc cctaccggct ataccgcact gctcggcggg ttaaccgaga acggacgtag
    15661 cgcggtgaac gtgctttcgt tcctttgcct tgacgcttat caaagcgtgc aattaccgca
    15721 accgaacctc ggcgtgcgca ctaacgcctt gatcgacacg ccgttcctga tgaaaaccgc
    15781 agaaaccatt cgcctcggca ccggtattcc gcaaatcttt aacgatgaag tggtggtgcc
    15841 agcgttcctc aaccgtggcg tttcgctgga agatgcgcgc gactattccg tagtgggctg
    15901 tgtggaatta tctattcccg gcagaaccta cggcttgcat gacatcgcga tgtttaacct
    15961 gctgaaagtg atggaaatct gcctgcatga aaatgaaggc aacgctgcgc tgacttatga
    16021 aggtttactg gaacagatcc gcgccaagat cagccactac atcaccctga tggttgaggg
    16081 cagcaatatt tgtgatatcg gccatcgcga ctgggcacct gtaccgctac tctcgtcttt
    16141 tatcagcgat tgtctggaaa aaggccgcga tattactgac ggcggcgcgc gttataactt
    16201 ctccggcgta caggggatcg gtatcgccaa cctgagcgat tctctccatg cgttgaaagg
    16261 gatgattttt gatcaacagc gtttaagttt tgacgaattg ctgtcggtat taaaagccaa
    16321 cttcgccacg ccagaaggcg aaaaagtccg cgctcgctta attaaccgct tcgagaaata
    16381 cggtaacgat atcgacgagg tggataacat cagcgccgaa ctgttacgcc actactgcaa
    16441 agaagtggaa aaataccaga acccgcgcgg cggctacttc acgccaggat cgtataccgt
    16501 ttctgctcac gtcccgttgg gatcggtggt tggcgcgacg ccagacggtc gttttgccgg
    16561 ggaacaactg gcagacggtg gcttgtcacc catgctgggt caggatgcac aagggccaac
    16621 ggcggtgctg aaatcagtca gtaagctcga taatacgctg ctgtctaacg gtacgttgct
    16681 gaacgtgaaa ttcactccgg cgaccctgga aggtgaagcg ggattacgca aactggccga
    16741 cttcttacgg gcgtttaccc agcttaagtt gcagcatatt cagtttaacg tggtgaacgc
    16801 cgacacgtta cgggaagcgc aacagcgccc acaagattat gccgggctgg tggtgcgcgt
    16861 tgccggatac agcgccttct ttgtcgaact gtcgaaggag atccaggatg acatcatccg
    16921 ccggacagcg catcagctgt aacgttgtgg aaacgcgccg caatgatgtg gcgcgcattt
    16981 tcaacattca gcgttattca ctgaatgacg gcgagggcat tcgtacggtg gtctttttta
    17041 aaggctgtcc gcatctttgc ccatggtgtg ctaatccgga gtcgatctcc ggcaaaatcc
    17101 agacggtgcg cagagaggcg aaatgtctgc actgcgcgaa atgtttgcgt gatgcggatg
    17161 aatgcccctc cggggcattt gaacggattg gtcgcgatat cagccttgac gctctggaac
    17221 gggaagtgat gaaagatgac attttctttc gcacgtccgg cggtggcgtc acgctttctg
    17281 gcggcgaagt gttaatgcag gcggagtttg cgacccgttt tttacagcga ctgcggctat
    17341 ggggtgtgtc atgcgccatc gaaactgccg gagacgcgcc agccagcaag ctgttaccgc
    17401 tggcgaaatt gtgcgatgaa gtgttgttcg atttaaaaat tatggatgcg gctcaggcgc
    17461 gggatgtggt gaagatgaac ctgcaacgcg tgctggagaa tctgcgtttg ctggtgagtg
    17521 agggtgtcaa cgtgatcccg cgtttaccgc tgatccctgg tttcacgctc agccgggaga
    17581 atatgcagca ggcgctggac gtgctgatcc cgctgaatat caagcagatc catctgttac
    17641 cgtttcatca gtacggcgag ccgaaatacc gcctgctggg gaaaacatgg tcgatgaaag
    17701 aggtttctgc gccgtcgtca gccgatgtgg caacgatgcg tgaaatggca gaacgggccg
    17761 gatttcaggt taccgtggga ggttaaaatg gcatatctgg tggcagtaac cgcctgcgta
    17821 agcggcgtgg cgcatactta tatggcggcg gaacggctgg aaaagttgtg ccagttagag
    17881 aagtggggcg ttagcattga aactcagggc gcgttgggaa cggagaatcg tttagcggac
    17941 gaggatatcc gccgggcgga tgttgcgctg ttaattacgg atatcgagct tgccggtgct
    18001 gagcgatttg aacattgtcg ctacgtgcaa tgcagcatct acgctttcct gcgtgagccg
    18061 cagagggtga tgagcgcagt gcgcaaagtg ctttctgcgc cgcagcaaac ccatcttatt
    18121 ctggagtagt cggtttttct gtcagttggc tgtggtactg ccgacgatat tccgacggcg
    18181 agcgttcggt atttttacga aacagacggc agaagtagtt gctgtcgaca aagccgcagg
    18241 cgtgcgccac ttcttttacc ttcaggtcat agcctttcaa taatgtctta gcgtgctcca
    18301 gtcgcgtgtg attcaggtat tcgttaaaac caatggcccc cgttttttga aacaggtgcg
    18361 agaggtaatt tggcgaaata tagaacgcct gtgcgacaga ttcgcgggta agtgcggagg
    18421 catagcgttc gtcgatataa tcacgaatag cttcgaatag agcctggctg cgtgaggcgg
    18481 tctgtatttg gctgccgagc aaatcgcggc agtggctaag caggctggtg acgataagtc
    18541 tggccgtttg ctgctcctgc gactgcattt gcatttcatt gagcgtttgt agcagaaaag
    18601 aaccaatgcg cgggccgcgt cgggcgacgt gttgcttcgc cagattttga tattgtttgc
    18661 catcccattg cacaacgctg aagccgagtt gctgtttgcc aaacagcacg ctaaaggtgg
    18721 tggcgggggc ttgccattgt ggaaaattcc agccaccagc agggacgtac agcacatcgc
    18781 cgggaactaa cgctgctcca gcgccggtat caataaactc accttccagc acaatttcca
    18841 gccgtggaaa atcgacctga tacgcaagat cgggcaccgg gccgttgctg ctggcaaagt
    18901 aaatctggcg cagggacagc gggccgttga tcaggcggga gagcagatag ctgacgtcgt
    18961 gatacatgtt attcccttag gtatgtagac gggaaaacag taactggcat tatgaaaaaa
    19021 ggccacctga aaagtgtggc ctgatgggag tgagatgtaa acattgcggc agtttcagtc
    19081 gacttgtatt gccggatgcg gcgtaaacgc cttatccggc ctaccgccgc gatctgtagg
    19141 ccggataaga cgcgtcaagc gtcgcatccg gcaaataaca tctggctgat aattactgat
    19201 tacccacctg atcgccatac ggcagagtgt cgtaatcgat cagtgcgttt ttcttatacg
    19261 ggttaccaat ccagcgggta gtttctttga actgcggatt caccactgaa cgggttggat
    19321 cgtaaccgtc gtaagataag cccgccaaat ctgaccaggt gtggatcagt tccgccaggc
    19381 tgtatttacg atcaacatcc tgtgagaaat cacgcggatg agtcgcttgc catttttctg
    19441 acgtccacag caggaacgga atggtgtaca tatgacgcgt cgggttgtct tcattacgcc
    19501 cctgagtttt gtgcggcggc gtgtcgtaaa cctcttcacc gtggtcagag aaataaacca
    19561 ggaaaccgtt cggatcggtc gctttaaagt ctttaatcag gctggcaacc acgtgatcgt
    19621 tgtacaggtt agcattgtca taatcattat atgactccag ctcttctgcg tttaatcccg
    19681 gtggaacatg ctctgtatta ccgtcaaatt tgccctgatc ttccggataa cggtacttgt
    19741 acttgatatg cgtacccagc agatgaacga tgatcagttt cttcggcgca gggtccttca
    19801 gcacttcctg gaacggcttc agcacgttgg tgtcgtattc acgcgcactc tgcgtacgtt
    19861 gctggttcat gtagtactgc ttatcggtct ggcgcgaaaa taccgtcagc atggtattgc
    19921 gggcggtcat cgtctgctgg ttggtgatcc agaaggtttt ataacccgcc tgtttcatca
    19981 tgttcatcag cgacggctgc gtcagataca gatccgggtt cttttcattg gcaaaggtca
    20041 gcgcctgttg caggatttca atggtgtacg gacgagacgt aaccacgtta ttaaacacgg
    20101 tcagattcgg atcggtttta tgcagcgcat ccagctccgg cgtggtttca cgcggataac
    20161 cgtacagact catgcgtcca cgctgggtcg attcgccaat taccagcact aaggtgcgcg
    20221 gttcgttacc cgattcatct ttgaaattag ccagcggcgg caaggcatta ttttcattca
    20281 gtagctttgt tagcgagttt agttgctgac gatactgata atagccggtc aggaattgcc
    20341 acggtgcggc aggctccata cgcgatgcca ggttatccaa cgttttctca aacggcttgt
    20401 tcttgataaa cgtattcatg gcgatcggat gcagaatcaa gccataaagc agggcaaaag
    20461 agacaacata acgccacggc tttggaatat agaccgggcg caggcgtgtc cacagcagaa
    20521 ctgccaccgc cgtataggcc agcgcgataa gcacaatttt caggctgaaa tactggctta
    20581 aatactcgct ggcttcgttg gtgttggttt cgaacatcac aaacagaacg ctctgcgaga
    20641 actcctgacc gtagatgacg taatagcaca gcgccgccag agaggccgcc catagcacca
    20701 cgccgatcac tgcggcaata attttaatcc gcttcggaaa gaggaatacc gggatcaacc
    20761 acagcgaact gaataacagc gagtcgcgaa tgccgttagt gccactataa ccactgatgt
    20821 aaataatggc ctgtagcaga gtagagaaaa accaaaagta gagcagtgcc caacccaggg
    20881 ctttccagct aaaaaggggt ttagcctgga cttctgtgga atgcatagta agaacctgtc
    20941 ttgaaaaaaa tatcgccgaa tgtaacgaca attccttaag gatatctgaa gggatatcca
    21001 gattttgaat aaaatgcaga cagaaatata ttgaaaacga gggtgttaga acagaagtat
    21061 ttcagaaaac cctcgcgcaa aagcacgagg gtttgcagga aaggaagatt agccggtatt
    21121 acgcatacct gccgcaatcc cggcaatagt gaccattaac gcctgctcga cgcgaggatc
    21181 cggttcctgg ccttcttttt ctgcctggcg ggagcggtgc agcaactcgg cctgcaatac
    21241 gttcagcggg tcggtgtaaa tattccgtag ctgaatagac tctgcaatcc acggcagatc
    21301 ggccatcagg tgagaatcgt tggcaatcgc cagcaccact ttgatgtctt cttcttgcag
    21361 attgcgtaac tctttaccta acggccacag tgctttgtct accaggcgtt ggtcatagta
    21421 ttccgccagc cacaggtctg ctttggcgaa gaccatctcc agcatgccga gacgcgtcga
    21481 gaagaatggc caatcgcggc acatggcttc cagctcgctc tgtttaccat cctccaccac
    21541 tttttgtagc gccgtacccg cgcccagcca ggcggggagc atcaggcggt tttgtgtcca
    21601 ggcaaaaatc cacggaatag cgcgcagtga ctcgacgccg ccggttgggc gacgtttcgc
    21661 cggacgtgaa cccaacggca gtttacccaa ttcttgttcc ggcgtagcgg agcggaagta
    21721 aggcacaaaa tctttgtttt cacgtacgta gccacggtag agatcgcagg agatgactga
    21781 cagttcatcc ataatgcgac gccagctctc tttcggctca ggcggtggca gcaggttggc
    21841 ttccagaatc gccccggtgt acagcgacag gctgctgacg gtgatttctg gcagaccata
    21901 tttaaagcgg atcatctcgc cctgttcggt cacgcgcagg ccgcctttca ggcttcctgg
    21961 tggttgtgac agcagcgccg catgggcagg tgcgccgccg cgaccaatgg aaccgccgcg
    22021 accgtggaac aacgttagct caatacccgc tttttcgcag gttttgatta atgcatcctg
    22081 tgcctggtat tgcgcccagg aagctgccat cacgcccgca tcttttgctg agtcggaata
    22141 gccaatcatc accatctgtt tgccctgaat caggccgcga taccagtcga tattcagcag
    22201 ctgggtcatg acatcgttgg cgttgttcag gtcatcgagg gtttcaaaca gcggagcaac
    22261 cggcatcgca aacccgatac ccgcttcttt cagcagcagg tggacagcca gcacgtcgga
    22321 cggcgttttc gccatcgaga tcacgtaggc ggcaatggag ccttgcggcg cttcggcaat
    22381 cacctggcag gtatcgagca cttcgcgcgt ttcggcgctt ggttgccagt tgcgcggtag
    22441 aagcggacgt ttggagttca gttcgcggat caggaacgcc tgtttgtcgg cttctgacca
    22501 gctctcgtag tcgccgatac cgaggtagcg ggtcagctcg cccagcgctt cggtatggcg
    22561 ggtgctctcc tgacggatat caatacggac cagcggtacg ccgaaacatt tcacgcggcg
    22621 cagggtgtcg agcagatcgc cgttggcgat aatacccatg ccacacgcct gaagtgactg
    22681 gtagcaagcg tagagcggtt cccacagttc ttcgttttgt gtcagcaggc cttctggttt
    22741 tggcagttct tcgcctttca ggcgcgcttc cagccatgcc tgtgtcgcca tcaggcgaga
    22801 acgcaggttt ttcatcagat agcgatacgg ttctgcggca ccttcttcgc caaccagcgc
    22861 cagcagttca ggggtcgctt caaccatcga cagttcagaa accagcacct gaatatcttt
    22921 caggaacaga tcggtggctt tccagcgact gagcagcagg acgtggcggg tgatatcggc
    22981 agtgacgttc gggttgccgt cgcggtcacc gcccatccac gaggtaaaac ggaccggaac
    23041 aaattcgacg ggcagtttgt agccgagatt ctcttccagt tgttcgttca gttcgcgcag
    23101 gtaattcggt acgccttgcc acaggctgtt ttctactacg gcaaagcccc atttggcttc
    23161 atctaccggg cttggacgca gtttacggat ttcatcggta tgccatgact gggcgatcaa
    23221 ctggcgcagg cggcgcatca gctggttatg ttcgtagtcg gcgatatctt tgttatcgag
    23281 ctgttttaag caggcgttca cttccaccat tttgtggatc agtgtacgac gggtaatttc
    23341 ggttgggtga gccgtgagga ccagttccag cgacagcgat tccactgctt tttttatggt
    23401 gtcttcgctc agttccggct ggtttttcag tttgcgcagg gtgcgggcga tcacttccgg
    23461 gttgctggca gcttcgcctt tcggcgaaat gctgtggtat tgctcggcgg tgttggccag
    23521 gttcagaaac tgactaaacg cacgcgcaac gggcagcagc tcgtcgttcg acaaattttg
    23581 taaggtggtg agcaactcct ggcggttagc atcattgcca gcgcgtgaag atttggacaa
    23641 cttacggata gtttctacgc gttcaagaat gtgttctccc aacgcatcct tgatggtttc
    23701 tcccagcact ttgccgagca tactgacatt actacgcaat gcggaatatt gttcgttcat
    23761 attaccccag acaccccatc ttatcgtttg atagccctgt atccttcacg tcgcattggc
    23821 gcgaatatgc tcgggctttg cttttcgtcg tcttttataa agccacgtaa aagcggtgac
    23881 gtcaaatgct gcgaaatcgc ttcagcaaac gaataaatag caggaattta cgccattaaa
    23941 ttcatgacgc tttaaataag cgtaacttat gggaatgtta aaaaatcgcc tcgagtaaca
    24001 ccaaaggtgt aggtcggata agatgcgcaa gcatcgcatc cgacattgtt tggcctacgt
    24061 tttaatgcca gcaaaaatgg tgaatcacct gggtgatcag ttcgcgggtg ggcttgataa
    24121 acctcgtctc cagatattca tcaggctgat gagcctgatt aattgagcca ggccccagaa
    24181 ccagcgtcgg gcataacgtt tgaataaacg gcgcttcggt acagtaattc accacttcgg
    24241 tctttgctcc gagcaatttc tcaaccactt caaccagttg atgattcggt gggcattcat
    24301 agccagggat cggcggatgc agctcgtcga ccgtcagacg acccggccag cgctcgctca
    24361 ctggagccaa tgcatcgttg agcaaaccat taagttcatt gagtgtcatg cctggcagcg
    24421 gacgaatatc catatgcaac tcacagcaag cacaaatacg gttagaagcg tcgccaccgt
    24481 ggatatgccc gaggttgagc gtagggtatg gcacggtaaa cgcgtcgtag tgataacgtt
    24541 ctttcaggtt atcgcgcaat tgcaaaatat gccctatggc gtcgtgcatc agttcgatag
    24601 cgttaactcc gcgtgctgga tcgctggagt ggcccgactg gccctgaata cggatggcgt
    24661 tagagatatg acctttatgc gcgcgtaccg gttgcagcga cgttggctcg ccaatgatgg
    24721 cgcaatccgg gcgcagggcg gtagtttcgg caaaataacg cgctccggcc atactggttt
    24781 cttcatcggc agtcgccaga atgtagagcg gttttgcaag cttcgtgacg tcgacatcgc
    24841 gtagcgcatc aaggataaac gcaaaaaagc ctttcatgtc ggcggtgcct aagccgtaaa
    24901 gcttgccgtc atgctccgtc aacgtaaatg gatcgcgcgt ccagcgtccg tcatcaaatg
    24961 gcaccgtatc ggtatgcccc gccagcaaca aaccgccagc cccctgtccg caactggcca
    25021 gcatattaaa tttgttgcga gttcctggca caggctgtac ttccacattg aagcctaaat
    25081 ccttaaacca gtccgccagc agagtgatta aatctgcatt gctttgatcg agtgcctctt
    25141 ccgtggcgct tattgaaggt gtggcaatca gagcacggta aatctcgata aatggcggta
    25201 atttgttttt cattgttgac acacctctgg tcatgatagt atcaatattc atgcagtatt
    25261 tatgaataaa aatacactaa cgttgagcgt aataaaaccc accagccgta aggtgaatgt
    25321 tttacgttta acctggcaac cagacataag aaggtgaata gccccgatgt tgaatacgct
    25381 gattgtgggt gccagcggct acgctggcgc agagctagtg acctatgtaa atcgccatcc
    25441 gcatatgaac ataaccgctt tgactgtttc agcgcaaagc aatgatgcgg gaaagttaat
    25501 ctccgatttg catccgcagc taaaaggcat cgttgatctg ccgttgcagc cgatgtcgga
    25561 tatcagcgag tttagcccag gggtggacgt ggtgtttctc gccaccgccc atgaagtcag
    25621 ccacgattta gcgccgcaat ttcttgaagc aggctgtgtg gtgttcgacc tttccggcgc
    25681 gtttcgtgtt aacgatgtcg ccttctatga aaaatattac ggctttaccc atcaataccc
    25741 ggaactgctg gaacaggcag cctacggtct ggcggagtgg tgcggtaata aattaaaaga
    25801 agcgaacttg attgccgtgc caggctgtta tccgacggca gcacagctgg cgctgaaacc
    25861 gttgattgat gccgatcttc ttgacctcaa tcagtggccg gtgatcaacg ccaccagcgg
    25921 cgtgagcggt gcaggacgta aggcagcgat ttcaaatagt ttttgtgaag ttagcctgca
    25981 accgtatggc gtctttaccc atcgccatca accagagatc gccacacacc tcggtgctga
    26041 cgttatcttc accccgcatc tgggcaattt cccgcgcggc attctcgaaa ccattacctg
    26101 ccgcctgaaa ccgggtgtga gccaggtgca agtggcgcaa gcgttacagc aggcgtatgc
    26161 ccataaacca ctggtgcggc tgtatgacaa aggcgttccg gcgctgaaaa atgtcgttgg
    26221 actgccattc tgcgatatcg gttttgccgt tcagggcgag catctgatta ttgtggcgac
    26281 cgaagacaac ttactgaaag gcgcggcggc acaagcggtg cagtgcgcca atattcgttt
    26341 cggctatgcg gaaacgcagt ctcttattta agggtgcaat gatgaatcca ttaattatca
    26401 aactgggtgg cgtactgctg gatagtgaag aggcgctgga acgtctgttt agcgcactgg
    26461 tgaattatcg cgagtcacat cagcgtccgc tggtgattgt gcacggcggc ggttgcgtgg
    26521 tggatgagct gatgaaaggg ctgaatctgc cggtgaaaaa gaaaaatggc ctgcgggtga
    26581 cgcctgcgga tcagatagac attatcaccg gagcactggc gggaacggca aataaaaccc
    26641 tgctggcatg ggcgaagaaa catcagattg cggccgtagg tttgtttctc ggtgacggcg
    26701 acagcgtcaa agtgacccag cttgatgaag agttaggcca tgttggactg gcgcagccag
    26761 gttcgcctaa gcttatcaac agcttgctgg agaacggtta tctgccggtg gtcagctcca
    26821 ttggcgtaac agacgaaggg caactgatga acgtcaatgc cgaccaggcg gcgacggcgc
    26881 tggcggcaac gctgggcgcg gatctgattt tgctctctga cgtcagcggc attctcgacg
    26941 gcaaagggca acgtattgcc gaaatgaccg ccgcgaaagc agaacaactg attgagcagg
    27001 gcattattac tgacggcatg attgtgaaag tgaacgcggc gctggatgct gcgcgcacgt
    27061 tgggccgtcc ggtagatatc gcctcctggc gtcatgcgga acagcttccg gcactgttta
    27121 acggtatgcc gatgggtacg cggattttag cttaagtttt gttggccgga ggcgcagctt
    27181 tccggcattg aatttcaaaa taaggaaaca gagttatggc actttggggc gggcgtttta
    27241 cccaggcagc agatcaacgg ttcaaacaat tcaacgactc actgcgcttt gattaccgtc
    27301 tggcggagca ggatattgtt ggctctgtgg cctggtccaa agccctggta acggtcggcg
    27361 tgttaaccgc agaagagcag gcgcaactgg aagaggcgct gaacgtgctg ctggaagatg
    27421 ttcgcgccag gccacaacaa atccttgaaa gcgacgccga agatatccat agctgggtgg
    27481 aaggcaaact gatcgacaaa gtgggtcagt taggcaaaaa actgcatacc gggcgtagcc
    27541 gtaatgatca ggtagcaact gacctgaaac tgtggtgcaa agataccgtt agcgagttgc
    27601 tgacggctaa ccggcagtta caatccgctc tggtcgagac cgcgcagaac aatcaggacg
    27661 cggtgatgcc gggttacact cacctgcaac gcgcccagcc ggtgacgttc gctcactggt
    27721 gcctggccta cgttgagatg ctggcgcgtg atgaaagccg tttgcaggac gcgcttaagc
    27781 gtctggatgt cagcccgcta ggttgtggcg cactggcagg aacggcctat gaaatcgacc
    27841 gtgaacagtt agcaggctgg ctgggctttg cttcggcgac ccgtaacagt ctcgacagcg
    27901 tttctgaccg tgaccatgtg ttggaactgc tttctgctgc cgctatcggc atggtgcatc
    27961 tgtcgcgttt tgctgaagat ctgattttct ttaacaccgg cgaagcgggg tttgtggagc
    28021 tttctgaccg tgtgacttcc ggttcatcat taatgccgca gaagaaaaac ccggatgcgc
    28081 tggagctgat tcgcggtaaa tgcggtcggg tgcagggggc gttaaccggc atgatgatga
    28141 cgctgaaagg tttgccgctg gcttacaaca aagatatgca ggaagacaaa gaaggtctgt
    28201 tcgacgcgct cgatacctgg ctggactgcc tgcatatggc ggcgctggtg ctggacggca
    28261 ttcaggtgaa acgtccgcgt tgccaggaag cggcgcagca gggttacgcc aacgctactg
    28321 aactggcgga ttatctggtg gcgaaaggcg taccgttccg cgaggcgcac catattgtgg
    28381 gtgaagctgt ggtggaagcc attcgtcagg gcaaagcgtt ggaagatctg ccgctcagtg
    28441 agttgcagaa attcagccag gtgattggcg aagatgtcta tccgattctg tcgctgcaat
    28501 cgtgcctcga caagcgtgca gcaaaaggcg gtgtctcacc gcaacaggtg gcgcaggcga
    28561 ttgcttttgc gcaggcgcgg ttggggtaat ttgtttatat tgctcccctg aaaatgggga
    28621 gcttaccacg cctacaaagt gtgacacttc gtaggcggat aaggcgttta cgccgcatca
    28681 ggcagaaatt tgtcagcatt ctggtgacct taaatttaac gcccaaccag aaccattatt
    28741 tgtcacattc ctttttattc ttgcgtcttc tttaccttca ctctccccat aaa
//
LOCUS       DADSZD010000043        28733 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.043, whole genome shotgun sequence.
ACCESSION   DADSZD010000043 DADSZD010000000
VERSION     DADSZD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.043"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            177..482
                     /locus_tag="J1Z28_001689"
     CDS             177..482
                     /locus_tag="J1Z28_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716530.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTGGVAAGAVGTATAVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            589..1233
                     /locus_tag="J1Z28_001690"
     CDS             589..1233
                     /locus_tag="J1Z28_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBA7716531.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1230..1976
                     /locus_tag="J1Z28_001691"
     CDS             1230..1976
                     /locus_tag="J1Z28_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBA7716532.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGMVTIYLPGEQQTLSVGPVEN
                     VAQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWLAGRSVTDYLQDHTRLAGADKNNVMVITPEGEAVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1976..4072
                     /locus_tag="J1Z28_001692"
     CDS             1976..4072
                     /locus_tag="J1Z28_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBA7716533.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAANWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     IWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4118..5257
                     /locus_tag="J1Z28_001693"
     CDS             4118..5257
                     /locus_tag="J1Z28_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBA7716534.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTITGVHDLTETSRWIQTWPN"
     gene            5245..5691
                     /gene="etp"
                     /locus_tag="J1Z28_001694"
                     /gene_synonym="yccY"
     CDS             5245..5691
                     /gene="etp"
                     /locus_tag="J1Z28_001694"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBA7716535.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5711..7891
                     /gene="etk"
                     /locus_tag="J1Z28_001695"
     CDS             5711..7891
                     /gene="etk"
                     /locus_tag="J1Z28_001695"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBA7716536.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKAGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITHILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PQVRSELDQAEEKLNAYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLASVIAQSDQKVLFI
                     DADLRRGYSHNLFNVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRYVGTSLLVARFSLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8006..9304)
                     /locus_tag="J1Z28_001696"
     CDS             complement(8006..9304)
                     /locus_tag="J1Z28_001696"
                     /EC_number="3.1.3.2"
                     /EC_number="3.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AppA family phytase/histidine-type acid
                     phosphatase"
                     /protein_id="HBA7716537.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRIADSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALAPHPPQKQVYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9384..9476)
                     /gene="cbdX"
                     /locus_tag="J1Z28_001697"
     CDS             complement(9384..9476)
                     /gene="cbdX"
                     /locus_tag="J1Z28_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBA7716538.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9489..10625)
                     /gene="appB"
                     /locus_tag="J1Z28_001698"
     CDS             complement(9489..10625)
                     /gene="appB"
                     /locus_tag="J1Z28_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBA7716539.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGVWMNNFVESPVLWIFPLLGVFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10637..12181)
                     /gene="appC"
                     /locus_tag="J1Z28_001699"
     CDS             complement(10637..12181)
                     /gene="appC"
                     /locus_tag="J1Z28_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBA7716540.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12315..13172)
                     /locus_tag="J1Z28_001700"
     CDS             complement(12315..13172)
                     /locus_tag="J1Z28_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBA7716541.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINMTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            complement(13169..13567)
                     /gene="hyaE"
                     /locus_tag="J1Z28_001701"
     CDS             complement(13169..13567)
                     /gene="hyaE"
                     /locus_tag="J1Z28_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBA7716542.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13564..14151)
                     /locus_tag="J1Z28_001702"
     CDS             complement(13564..14151)
                     /locus_tag="J1Z28_001702"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBA7716543.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESAGHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            complement(14148..14855)
                     /gene="hyaC"
                     /locus_tag="J1Z28_001703"
     CDS             complement(14148..14855)
                     /gene="hyaC"
                     /locus_tag="J1Z28_001703"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBA7716544.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14874..16667)
                     /gene="hyaB"
                     /locus_tag="J1Z28_001704"
     CDS             complement(14874..16667)
                     /gene="hyaB"
                     /locus_tag="J1Z28_001704"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBA7716545.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFSEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16664..17782)
                     /gene="hyaA"
                     /locus_tag="J1Z28_001705"
     CDS             complement(16664..17782)
                     /gene="hyaA"
                     /locus_tag="J1Z28_001705"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBA7716546.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18208..18295
                     /locus_tag="J1Z28_001706"
     tRNA            18208..18295
                     /locus_tag="J1Z28_001706"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18242..18244,aa:Ser,seq:tga)
     gene            18502..19161
                     /gene="yccA"
                     /locus_tag="J1Z28_001707"
     CDS             18502..19161
                     /gene="yccA"
                     /locus_tag="J1Z28_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBA7716547.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            19253..19582
                     /gene="tusE"
                     /locus_tag="J1Z28_001708"
     CDS             19253..19582
                     /gene="tusE"
                     /locus_tag="J1Z28_001708"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBA7716548.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(19579..19857)
                     /gene="yccX"
                     /locus_tag="J1Z28_001709"
     CDS             complement(19579..19857)
                     /gene="yccX"
                     /locus_tag="J1Z28_001709"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBA7716549.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGDEGQVEKLIQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            19952..21142
                     /gene="rlmI"
                     /locus_tag="J1Z28_001710"
     CDS             19952..21142
                     /gene="rlmI"
                     /locus_tag="J1Z28_001710"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBA7716550.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSHEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            21200..21517
                     /gene="hspQ"
                     /locus_tag="J1Z28_001711"
     CDS             21200..21517
                     /gene="hspQ"
                     /locus_tag="J1Z28_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBA7716551.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(21562..21975)
                     /locus_tag="J1Z28_001712"
     CDS             complement(21562..21975)
                     /locus_tag="J1Z28_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBA7716552.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            22148..22810
                     /locus_tag="J1Z28_001713"
     CDS             22148..22810
                     /locus_tag="J1Z28_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBA7716553.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22906..23364
                     /gene="mgsA"
                     /locus_tag="J1Z28_001714"
     CDS             22906..23364
                     /gene="mgsA"
                     /locus_tag="J1Z28_001714"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBA7716554.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23396..25450)
                     /gene="helD"
                     /locus_tag="J1Z28_001715"
     CDS             complement(23396..25450)
                     /gene="helD"
                     /locus_tag="J1Z28_001715"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBA7716555.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLTEYADFFRQVESSLLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVDILKNLDVPVAR
                     KP"
     gene            25573..26019
                     /locus_tag="J1Z28_001716"
     CDS             25573..26019
                     /locus_tag="J1Z28_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBA7716556.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHVATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            26029..28191
                     /gene="yccS"
                     /locus_tag="J1Z28_001717"
     CDS             26029..28191
                     /gene="yccS"
                     /locus_tag="J1Z28_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBA7716557.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(28154..28729)
                     /gene="sxy"
                     /locus_tag="J1Z28_001718"
     CDS             complement(28154..28729)
                     /gene="sxy"
                     /locus_tag="J1Z28_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBA7716558.1"
                     /translation="MGTIEYRSLFGSYSLTVDDTVFAMVSDGELYLRACEQSAPYCVK
                     HPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYSLDAALKEKSTRNIRERLK
                     DLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQNSLVTEKILFMLEGAIIGI
                     HEAALPVARRQELAEWADSLTPKQEFPAELE"
BASE COUNT         6887 a         7609 c         7311 g         6926 t
ORIGIN      
        1 atttccagag ggaaatcgtt aacaatctta tatttccctc tgcgcgcctt tctcacccgc
       61 agtataaagt gcaagcggta tgaggaaagg gagatttcac aaggatgtta tactcaaagt
      121 agataatcct gatctcaagc gtacgtattg tccatgcgat gaataaagga aaagttatga
      181 aacacaagct ttctgcaatc cttatggcct tcatgttgac gacgccagct gcatttgctg
      241 cccctgaagc agcaaacggg accgaggcaa cgacgggtac aactggcacc acaacgacca
      301 ctaccggtgc aaccacgact gcagctacca ctggtggcgt ggctgctggc gctgtcggta
      361 cggcgacggc tgtcggtgtg gcaacggctg ttggtgtggc gacgttggca gttgttgcag
      421 caaatgacag tggcgatggc ggttctcata acacctcaac caccacgtcc actacgcgct
      481 aataccactt taacgcgtcg aaattaaagt taaatgatac ccggttgcga tattcgtacc
      541 gggctttaga gatttgcatt tttttaatta acctttcaag gattaaaggt gcgccctctt
      601 attttatcga ttttcgcact atttcttgcg ggatgtacgc acagccagca aagtatggtc
      661 gatacatttc gcgccagcct attcgataat caggatatca ccgtagcgga tcagcagatc
      721 caggcgttgc cttattccac gatgtattta cgccttaatg aagggcaacg aatctttgtg
      781 gtactgggat atatagaaca agaacaaagc aaatggttat cccaggataa cgccatgctg
      841 gttacccaca atggacgtct tttaaaaacc gtcaaactta ataataatct gctggaagtg
      901 acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa taaaagacgg cagccgctgg
      961 acgcgcgata ttctctggag cgaagacaac cattttcgct ctgcgaccct gagttccact
     1021 ttttcctttg ctggattaga gacgctgaat attgcgggtc gcgatgtgct gtgtaatgtc
     1081 tggcaggaag aggtgacttc cacgctgcca gaaaaacagt ggcaaaacac attctgggtc
     1141 gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt taggcgcagg ggttattccc
     1201 gtagaaatga cgtttcttaa acccgcacca tgaataaatt acagtcgtat ttcattgcca
     1261 gcgtccttta cgtaatgaca ccccatgcct ttgcgcaagg aatggtgact atttatctgc
     1321 ctggcgaaca acagacactt tccgttggcc ccgtagagaa tgttgcccag ttggtgacac
     1381 aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt gctgaccgac tccgcagcga
     1441 aagctaaagc gcttaaagac taccaacatg tcatggcaca gcttgcttcc tgggaagcag
     1501 aagctgacga cgatgtggcg gcaacgatta aatccgtgcg ccagcagtta ctcaatctca
     1561 atatcactgg tcgcttaccg gtcaaactgg accctgattt tgtacgagtg gatgagaaca
     1621 gcaatccccc gctggtcggg gactatacgc tctataccgt acagcgtccg gtgactatta
     1681 ctctgctggg tgcagtttct ggggctgggc aattgccctg gctggcaggc cgtagcgtta
     1741 ccgattatct gcaagatcac acccgtcttg caggcgcgga caagaataac gttatggtga
     1801 ttacgcctga gggtgaggct gtcgtcgccc ctgttgccct gtggaataaa agacatgtgg
     1861 aaccaccgcc tggcagccag ctttggttag gcttttctgc gcatgtactg cccgaaaaat
     1921 atgctgattt aaatgaccag attgtgtctg ttttaacgca acgtgttcct gactgatgaa
     1981 gaagaactct tatcttttaa gctgcctggc tattgccgtc tccagtgcct gtcatgctga
     2041 agtattaacc tacccggatc cgctgggttc gtcgcaatca gactttggcg gcacaggatt
     2101 gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa ttcagcgtca actaccggga
     2161 taacgatcag taccgtttct actccacgtc cgtggcgctg ttcccatggc tggaaggcac
     2221 cattcgttat acggatgtgc gcacacgcaa atatagccag tgggaagatt tcagcggcga
     2281 tcagtcatac aaagacaaat cattcgattt taaacttcgc ctgtgggaag aaggttactg
     2341 gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt acgggtctgt tcgacggtga
     2401 atatctggtc gccagcaaac aagccggacc gttcgatttc accctcggga tggcctgggg
     2461 ctacgccggt aatgcgggca atattaccaa cccgttttgt cgggtaagcg ataaatattg
     2521 ccatcgcgca gagtctcacg acgcgggaga tatcagcttt agcgatatct ttcgtggtcc
     2581 agcctctatt tttggcggta ttgagtatca aacgccgtgg aatcctctgc gtctgaaact
     2641 cgaatacgac ggcaacaatt accagaatga ttttgcgggc aaattgccgc aggcaagtca
     2701 tttcaatgtt ggtgctgtgt atcgtgctgc caactgggca gatcttaacc ttagctacga
     2761 gcgtggtaac acgttgatgt ttggcttcac gttacgaacc aatttcaacg atctgcgccc
     2821 tgccctgcgc gatacgccaa aaccggcata tcaacctgcg ccggaatcgg aaggattgca
     2881 gtacaccacg gtagcaaacc aacttaccgc actgaagtac aacgcaggtt ttgaagcacc
     2941 ggaaattcag ctacgcgata agacgctgta tatgtctggt cagcaataca aataccgtga
     3001 ctctcgcgaa gcggtcgatc gtgccaaccg gattctggtg aataacctgc cgcaaggcgt
     3061 tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg atggtgacta ccgaaaccga
     3121 cgtagccagc ctgcgcaaac agttggccgg tacagcgcct ggtcaatcag agccactgca
     3181 acaacaacgt gttgaagctg aagatctttc tgcctttggt cggggctatc gtattcgtga
     3241 agatcgcttt agctactctt tcaacccaac actttcacag tcgctgggcg gcccggaaga
     3301 tttctatatg ttccagttgg ggctgatgtc cagcgcccgc tactggttta ccgaccacct
     3361 gctacttgat ggcggtattt tcaccaatat ttacaacaac tacgacaagt ttaagtcttc
     3421 gctgttaccc gcggactcta ccctgccccg cgtgcgcacg catatccgtg attacgttcg
     3481 caatgacgtt tatctcaaca acttgcaggc gaactacttt gccgacttag gcaatggttt
     3541 ctatggccag gtgtatggcg gttatctgga aacgatgtat gccggtgtcg gttccgagct
     3601 gctttatcgc ccgctagatg ccagctgggc gctgggtgtg gacgttaact acgtgaagca
     3661 gcgtgactgg gacaacatga tgcgcttcac cgattattcc acgccaacag gtttcgtgac
     3721 ggcttactgg aacccgccga cgctgaatgg tgtactgatg aaactcagcg tggggcaata
     3781 tctggcaaaa gataaaggtg caacgattga cgttgccaaa cgctttgaca gcggtgtggc
     3841 agtaggaata tgggcggcaa tcagtaacgt atcaaaagat gactacggcg aaggcggctt
     3901 tagtaaaggg ttttatatct cgattccgtt cgacttgatg accattgggc ctaaccgcaa
     3961 ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt ggacaaatgc tgtcacgcaa
     4021 ataccagctc tatccaatga cggcagagcg agaagtaccg gttggacaat aacctgaagt
     4081 aattcataac attacaatga ctgataaata taaaccaatg aaaaagaaca tttttaagtt
     4141 ttcggtactg acattggctg ttctgtcttt gacagcatgt acccttgttc ctggtcagaa
     4201 tttatcgacc agtaacaagg atgtcattga actacctgac aatcagtatg acctggacaa
     4261 aatggtcaat atctatcccg tcacgccagg attgattgat cagctgcgcg ccaaaccgat
     4321 tatgtcgcag gctaatccgg agctggagca gcaaatcgcg aactatgaat atcgcatcgg
     4381 cattggtgat gtgttgatgg ttacggtctg ggatcacccg gaactaacca cccccgcagg
     4441 gcaataccgt agcgccagtg ataccggcaa ctgggttaac gccgatggtg cgattttcta
     4501 cccctatatt ggtcgcctga aagtggccgg gaaaacactt acccaggtac gtaacgagat
     4561 tacagcgcgt ctggattcag tcattgaaag cccgcaggtc gatgtcagcg tcgcggcgtt
     4621 ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa tcgggtcagc agcctattac
     4681 caatattcct ctgaccatca tggatgcgat taacgccgcg ggaggtctga ccgcagatgc
     4741 agactggcgc aacgtggtgc tcactcagaa tggcgtaaag accaaagtga acctctatgc
     4801 gctgatgcag cgtggcgact tgcgccagaa caaactgctg cacccgggcg atattttatt
     4861 tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt gaagtgggta aacagagcac
     4921 gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg ctgggtaacg ctgagggtat
     4981 gaatcaggat gtcgccgacg cgacggggat ttttgttatt cgtgcaacgc aaaacaagca
     5041 gaatggcaaa atcgccaata tttatcagct gaatgcgaag gacgcatcgg caatgatact
     5101 cggaacggaa ttccagcttg aaccttacga cattgtttat gtcactactg cgccgctggc
     5161 tcgttggaac cgtgtgattt ctctgctggt accgacgatt accggggtgc atgacctgac
     5221 agagacttca cggtggattc aaacatggcc caactaaaat ttaactcaat cctggtggtt
     5281 tgtaccggca acatttgccg ttcgcccatt ggcgaacgct tgctgcgtaa acgtctgcca
     5341 ggcgtaaaag tcaaatcagc aggtgttcat ggtctggtaa aacaccctgc agatgcgaca
     5401 gcggcagatg ttgctgctaa tcatggcgta tctcttgaag gacatgccgg acgtaagctc
     5461 actgctgaaa tggcacgaaa ttacgatctg attctggcaa tggagtcaga gcatattgct
     5521 caggttaccg caatcgctcc cgaggtccgc gggaagacaa tgctgtttgg gcaatggctg
     5581 gaacagaaag agatcccgga tccctatcgt aaaagtcagg acgcatttga acatgtctac
     5641 ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc tcagccggta atttgagttt
     5701 ataaaatacg atgacaacta aaaatatgaa tacgccacca ggcagcactc aggaaaatga
     5761 gatcgatctg cttcgtctgg tcggcgagtt atgggatcac cgtaagttta ttatcagcgt
     5821 gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg ttaagcacgc caatttatca
     5881 ggcagatact ctggtccagg ttgagcagaa acagggcaac gccattctca gcggcctgag
     5941 cgatatgatc cctaactcat ctcccgagtc tgcaccggag atccaactgc tgcaatcgcg
     6001 catgattctc ggtaaaacca ttgctgaact gaatctgcgc gacatcgttg agcagaagta
     6061 ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa gagaaaccag gtgagctggc
     6121 gatcagctgg atgcatatac cacaactgaa tggtcaggat cagcaactga cgctgacggt
     6181 tggggaaaac ggccactata cgctggaagg tgaagagttc accgtcaatg gtatggtcgg
     6241 ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg gacattaagg ccaaagcagg
     6301 aacacagttt gtcctgagcc agcgcaccga actggaagcg attaacgccc tgcaggaaac
     6361 ctttaccgtt agcgagcgca gtaaagagag cgggatgctg gaactcacca tgaccggtga
     6421 cgatccgcaa ctgattactc atatactgaa cagcatcgct aacaactatt tgcagcagaa
     6481 tatcgcgcgt caggcggcac aggattcgca aagtcttgag ttcctgcagc gccagttacc
     6541 gcaggtacgc agtgagttgg atcaggctga ggaaaaactg aacgcctatc gtcaacaacg
     6601 tgattcggta gacttgaatc tggaagcaaa agcggttctt gagcagattg ttaatgttga
     6661 taatcaactc aatgagctga ctttccgtga ggcagagatc tcccagctat ataagaaaga
     6721 tcacccgacc tatcgtgcgc tgctggaaaa acgccagacg ctggagcaag aacgcaaacg
     6781 cctgaataag cgggtatcgg caatgccttc cactcaacag gaagtgctac gtttaagccg
     6841 tgacgtagaa gcaggccgtg cggtatatct gcaattactt aaccgccagc aggagttgag
     6901 tatttcgaaa tccagtgcca ttggtaacgt gcggattatc gacccggcag tcactcagcc
     6961 gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt ggttttattc ttggcctgtt
     7021 tatttcggtg ggtgccgtgc tggcgcgtgc gatgttgcgt cgtggtgtag aagcaccgga
     7081 acaactggaa gagcacggca tcagcgttta tgccactatc ccgatgtccg agtggctgga
     7141 taaacgcacc cgtctgcgta agaaaaattt attttctaat cagcagcgcc atcgtactaa
     7201 aaatatcccc ttcctggcgg tggataaccc ggcggattct gctgtggaag ccgtacgagc
     7261 actacgaacc agtctgcatt tcgctatgat ggagacggag aataacattc tgatgatcac
     7321 cggtgcgacg ccagacagtg gtaaaacgtt cgtcagttca actctggcgt cggtgatcgc
     7381 ccagtccgat caaaaagtgt tatttattga cgccgactta cgccgcggtt attcgcataa
     7441 cctgttcaac gtgagtaatg aacatggttt gtctgaatat ctggcaggta aagatgagct
     7501 caacaaagtg atccagcatt ttggcaaagg aggctttgat gtgattactc gcggtcaggt
     7561 gccacctaac ccgtctgaac tgctgatgcg cgatcggatg cgtcaattac tggaatgggc
     7621 gaacgaccat tacgatctgg tgattgtcga tacgccgccg atgctggcgg tgagcgatgc
     7681 tgcggtcgtg ggtcgctatg tcggcaccag cctgctggtt gcgcgtttta gcctgaacac
     7741 cgccaaagag gtgagcttgt caatgcaacg tctggaacag gcaggcgtca atattaaagg
     7801 cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct tacagttacg gctataacta
     7861 ttacggttat agttactccg agaaagagta aaactgaaag tggccggatg cggcgtgaac
     7921 gccttttccg gcctacgcat cagacagttc ttcgtttgtc atcaacctca gagcattcag
     7981 gtaactgaat gctctttttt atgcattaca aactgcacgc tggtatgcgt gcttcattca
     8041 cgatttgcgt aaaaccggcc aacgaacaca tgccctgcgc atttcgctct tcacatcctg
     8101 ccagggtcag tttcacctct ccgggcggcg tatttaatga cagcggcgtt ttatcacgca
     8161 tctgctgtaa agtctggaag accagcgaaa cctgaatcca ctggctgtta tcgctgagcc
     8221 gacgccagcg ttcaaacact agttcaccac ctggcggcgt gttatccggc tgacctggaa
     8281 gcgtccagtt gagctccagt gcgccgccga gatttgccag attagtatcg tgtccggcga
     8341 taaacagcac tgaagtgggt aatgtcacac catacacctg tttttgcggt ggatggggcg
     8401 ccaacgctgt catgatcaaa tccaataacg gggtggcgcg actgcgggca acctctggtg
     8461 tgcgttgtag taaataaaat tgcgcgttat gtagacttag caaggtgttc cactggtgtg
     8521 aatcagcgat ccttccccac cccggctccg gcattccttg tgcctgttgc aggagaaata
     8581 tctccgtcag cattgatgcg aggcttaccg caccggttaa tgagacattg tcggcgctca
     8641 ccttgagttc cgatggtaat gcctgcgtta atgaacagct ttcgtcctgt ttctcacggt
     8701 taaggcacaa gtttgattgc gggaaattaa gcacccgttc cagttcacga aacgccgttt
     8761 gccgatgccc ggtaaagtca gcaattgacc ctcctgccct gctgaggatc gcgtcagtca
     8821 cgttcgcgtt atccagttgg caaacgccag tttttagagg attaaataac ggatcgggac
     8881 tggaggtatc tgcctgggta tgtacggtta ttgcacagtc aggtgccagc ccggcggcga
     8941 aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc aataatcgcg acctgaccag
     9001 gctgcgggca gccctttttc gtcaacaatc cgtcggctac cagacgctgg cgttggtaat
     9061 gtccgagata agcgattaac tcaccaccgc gcggtgtcag ccaacccagt tttaccggcc
     9121 aggttggcca tgcgtctggg gtgacatcct gcatcagttg cgtggccttg gttggtgcac
     9181 gcacaccgtg acgactgaca atcaccacac tttccagctt cagctccggc tcactctgag
     9241 cgaatgcaga tttcggtgtt aacggaatca gaagagataa gaatgggatc aagatcgctt
     9301 tcatcgatat gtttccgctt ttcatatccg acattattga ttgcctgatg cgacgctaat
     9361 gcgcctcatc aggcttacct tcgttaactt ttcaatcgcg ggtccagcca taccaacaca
     9421 agggtggaga gcgaacacat caacaaaatg ccgacgaacc aaagtaaata ccacattgtt
     9481 cctgctcctt agtacagctc gttttcgtta cggcggagag tttctgtcgt catgcgcccc
     9541 cacattttgt agtagctcca gagagtgtag agcaacacaa tgggcaaaaa tatcagcacg
     9601 attaccaaca taatgctcag cgtcagctga ctggaagtgc tgtcccacaa cgtcaggctg
     9661 gagatcggac tcacgcttga cggcatgaca aaggggaaca gcgtgatgcc tgccgtgaaa
     9721 atcacgccaa attgcatcaa tgacgccatc aaaaatcccc aacccgggcg accacgataa
     9781 atcgccatca ccgtcagcaa tgggcagaag acccccagca gcgggaagat ccacaaaacg
     9841 ggcgattcga cgaaattatt catccagaca ccaggtagca ctgccaccag tttcattaac
     9901 ggattggaag gaccgttagc atcctgggcg agcagtacaa agccatcaat accaacccac
     9961 agccagtaac ccgccagcaa aaagcacagc atcaccagta gtgcggcgcg tttggtcgcc
    10021 agctgtgaac gcagatgaat cacaccaaca gttttcagtt gtaaccagac gccaccttgc
    10081 aaaatcacca tcccaagact gagcaatcca cacaataaag ggaatggcgt cagcagttgc
    10141 cagaagctgc cgagatattc cacgcgtaat tgcggtgtga aagcaaacgg cacgccgagc
    10201 agcaagttgc cgaatgcaat accgaagact accggcggca ccagactgcc gatgaccagt
    10261 ccggcatccc acattttacg ccagcgtgca tcggcgattt ttccgcgata atcaaaggcc
    10321 agcgggcgga agaacagcga gcacagcacc agaatcatcg ccacataaaa gccggaaaac
    10381 gccgctgcat acactctggg ccaggcggca aataatgccc cacctgcgag gatcaaccag
    10441 acctgattac cttcccagtg cgcgccaacg ctgtttatca ctatccgacg ttcatcatca
    10501 ttacgcgcca ccagcggcag cagacagccg atccccatgt caaatccgtc ggagatcata
    10561 aagaccacca ggatcacgcc aataagcagc caccagatga agcgcaatgt ttcataatca
    10621 aacatgattt tctcctttac ccctgttgct gcgtcggttg ttcactctgc atcgcgctcg
    10681 gccccagacg ggcatatttc tgcatcaggt agacttcggc gattaagaac agggtgtaaa
    10741 gccctacgat catgatcagt gagaaagcca gttgtcctgt ggttaaggcg gagtgcgcgg
    10801 agtatgtcgg taagatgtct tgtatcgccc acggctgacg accaaactcg gtcataaacc
    10861 atccggcttc aatcgcaatc cacggcagcg gcaaactcca gagcgccatt ttcagcaccc
    10921 agcgatgctg gtcgattttg ccacgcagcg tctggacaag cgcaatcagc atcaccagta
    10981 gcagcaggga accacagccc accatgatgc ggaaactcca gaataccggc gcaacctgag
    11041 gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt gacatgattc atatccggcg
    11101 catagcggga gagcaacatg ccgtaaccca ggtcgccttc cagctcccgg aatgcctgca
    11161 acacatgcgg ctcacgattg ccttgcgata tttcctgcat taacagccac gccatacgtc
    11221 cgcgttgcaa gcgtgggtag gtttcagcca tcaaattctt cagacccggc acgggtttat
    11281 ctaatgaatg agtggcgagg attcctagca gcgcgggaat tttgatggca aaggcgttac
    11341 gctcttgatc ctgttccggc caggcaacca catggaacgg tgcaggtgca ggttccgttt
    11401 gccactcccc ttccatcgcc gccagtttta ccggttgtac ttgcgcgact tcatacgcag
    11461 aactgtctcc gagttgcagc gtaccgataa tcgccagagt accgaagacg gaaccgatgg
    11521 caaacgagcg taatgcgaca tcgcgctccc gtccgcgcag taaataccag gcgctgatcg
    11581 ccataataaa catagccccg gtcacgtatc ccgccattac ggtgtgcaca aatttcacct
    11641 ggctgaccgg gttaaagacc agctcgctga aactggtcat ctccatacgc agggtgtcga
    11701 tatcaaaatg cgcaccagtc gggtattgca tccaaccgtt ggcattcaat atccacaacg
    11761 cagagagatt tgaaccgaac gccaccagcc acgtcaccag caggtgctgg tatttattca
    11821 gccgctgcca gccgaagaag aacagcccga caaaggtgga ttcgaggaag aaggccatta
    11881 atgcttccat cgccagcggt gcgccaaaaa tatcgcccac atagttggaa tagaatgacc
    11941 agttagtacc aaactgaaac tccatggtca ggccggtagc cacgccaaga gcaaagttga
    12001 taccgaagag cttaccccag aagcgcgtca tatcgcggta gattgttttg ccggtgacca
    12061 cgtaaatggt ttccatgata gccagcaaaa aaatcagccc cagggtaagg ggtacaaata
    12121 aaaagtgata cagcgcggtc agagcaaact gccagcgcga taaatcaatg acatcccaca
    12181 tgcgcactcc tgtaggcgaa gagagaaaaa taacccgtta atggtagatc tccctcttta
    12241 tcctgaaact ctacgaccag aggtattcgc cgattgcgat attcataagc ctgaaagcga
    12301 tacttaccgt ctttttacgt cggtgccccc tccgccagcc actggcatac ctcgctaagc
    12361 cgctgcgcag agtcgaccaa atcttctggc gctgccagca ccacttccgg tattgggcag
    12421 atttcataac tttccagtaa cgggcctttt aaggtgtccg tacagcgtaa atgccagaca
    12481 tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc caatggtacg aatctgaata
    12541 tttcccggcc cacagagacg tgagagaaaa agccggtcag cttcgctgat gggtaattgc
    12601 gtcatattaa tgctgtgcgg ctgcgcgtcg gggttacgta catgtgccag taattcatgg
    12661 gccaacggta ggccattcat caggccatca atgggcggcg gtaacagcga atctgtcggc
    12721 aagacgtttt gcgttgccgc ctgccacaac gccagcggtg cgcagccagc ctccagtttg
    12781 tcttccagca actgttcgcc gtgacgtctg cgtacccgcc ataatccgca gaagatcgcc
    12841 tcctgaattt cactttcact gtcgtcagcc tgctgaatgc gcactgacac ttccccttcc
    12901 ccgagaagcg tattgataaa gtgacgatcg tcggcgttga gatttaacag atcgactgcc
    12961 agtacctcac ccagcggcgg ctggcgttcg cttagttgat gttgtaactc gtttaacagc
    13021 gcaatcacct gcgggctgtg agcacattgc tccagggccg ccatactcgg ttcaccattc
    13081 acccgacagg tgatcggcag tggattcatg ctgaaactgt catcgttcgg ttgcgttcct
    13141 ggccccagca gatggaaaaa agtttcgctc atgaagcacg ctcctgttgc ggttcgacaa
    13201 gcccgcgcat caggtttatc agttccgccc acgggtgaat accattcagc acgccgcgat
    13261 agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa gacgccaaag cgatcgccga
    13321 tggcttcgct ctgctcaaga tcagcaatcg ctacctgcca tgtatagtcg ggaaattcgc
    13381 gcagtaattc gccaatcatt accgggttat cgctgacttc tggcgtgcgt ttcgggtcac
    13441 tgcttaataa caccacgccg tctggcgctt gcgtaagcca gtcgtcaaga cggcattcac
    13501 tgaccggcgt ccagccacgt gccagcattc gttgccacaa cgcattaaat ggtgtgtcgt
    13561 tgctcatccc tgttcctcca gcctcatcct gtactgacgc aagcgaacgc cttcgtaatt
    13621 ttccatcgac agacagtcat aattgagaca gcgcgattca ttagccggtt gcggcacaat
    13681 tccccacgca gccagctgcg ccagcgccgc ctgttccgca gcgggcagtt gctcccgcgc
    13741 cagttcgctc aggctgccgc cgtagtcgtc gagcatcgcg ggttgcagac cgacgagggc
    13801 aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc agcacttcgg agaaactgtt
    13861 ctggtgcagg ctcattttct tcgcgctgag ataagccgga atgcgttctc cggcatacgt
    13921 tcgtagcgtt ccgggttcca gcccgtaatc aatggcatcg agaatcaaca gatgaccggc
    13981 gctttcgaca taccccagca agttcagtcc ttgggtgccg ccatcgacaa tctccacatc
    14041 ctcgggccag tggtaatggg catacagccg ttccgccacc cgcacgccga agccttcatc
    14101 ggcccacagc aggttgccca gccccattac caccacgcgt tgctcgctca tgaacgctcc
    14161 ttgttactta ttttgccaaa tttgtggcta cggtagccgt tgaccatagt ggagatcacc
    14221 gtgtcgtcgg acatgatgtc ttcacgcagc gccatgtaga catgaccgat cacaaacgcg
    14281 ccaatcagcc acatccccag ccgatgccag ctgtgaatgt ccattgagtt gccacccgtc
    14341 cagtagaaaa attccaccac ataacggaac ggcgcaaaaa tagcgtactg gctgtgttcg
    14401 ctgtacagcg caaagccggt gatgatcata aaaactgaca tcaggaaata gccgaacatc
    14461 gccgcctggg cgatggggtt atggcctata tcggcactcg gacgttttgc cagaaacaga
    14521 taccagcgga tttcatacca cacgccctgc caccagcttt tacgccatac cggcacgata
    14581 aacagctcgc gggagtaacg attgccaaca aaagcccagt agatccgcat cagcaagacc
    14641 acggtaaaaa tcatcccggc gctgaagtga attaacctga tgtagcccat atagaacaga
    14701 tacgtcgcct cgccgctgac ggaaggtagc ggcttgccga taaagtaacc ggtgaccatc
    14761 aacaccgcca tgcataacac tgtcagccag tgccatatcc gcacgggtgc ttcaaagaca
    14821 tagtgactga caacgttgtc gcttttctgt tgcatgatga ttctccttcg ctgttaacgc
    14881 acctgcacgg agataagctc gctaccgtcg tcgcccagca cgtgtgttga acaggcgagg
    14941 cacgggtcaa agctgtgcag agtacgcagg atctccagcg gttgctcggg gatcgccatt
    15001 ttggtgttca tcagcgccgc ttcataagcg ccaatctgcc ctttgggatc gcgcgggctg
    15061 gcgttccagg tggtcggcac cacgcactgg tagagatcaa tcttgccatc gcgaatggca
    15121 gcccagtggc ctaacgcccc gcgcggcgct tcggtaaaac cgacaccacg gcactctgtc
    15181 ggccaggttg ctggttccca tttttccgtg gaagcggtgg caagattgcc gtttttcagg
    15241 ttggtcatca gtttatcgaa gaaatactgc aacttacctg cggcccactg cgcttcgtgc
    15301 gcgcgacaca aaatgcggcc caacgttgac tggatgccgg aaagcggcag tttcaacgcc
    15361 gacatcatac gatcgaccga ctcaacggtc gcagcatcgc ctttgtgata agcgattaac
    15421 gtgcgtgcca gcggccccac ttccatcgcg ttaccgcgcc agcgtggcgc tttgatccac
    15481 gagtagcgtt cctgttcatt cagctgctga atgttggtat cgctgccttt gacatcgccg
    15541 gggttgtacc acggatcggt aatgccatcg aacggatgac gccccacctg atcgttggga
    15601 taacgatacc aggcgtggtc gacaaactcc tgcacctgct gcggatcaac caaatccact
    15661 ggcagcacat tgttgaagtc gccgttaatc accgcgccgc caggcatcag cagacttttc
    15721 tcactaaagt cgttggcaat atccgggaat gcgccgtagc tgaggacaca tttatcagaa
    15781 agacccgtgc cgatttcgct ccacggtttg ttgaactggc cgatggctaa ggcgtcgggg
    15841 atcatcacgt tgttaatgaa gtccgccgta cgggtaatga tcgactgcac caggttcagg
    15901 cgttccatat tgaccgcccc gactgcgccg ctttcgtcaa tgttaatggc gcaaggcatc
    15961 ccgccgacaa tccagtttgg atgcgggttt ttaccaccaa agaccgcgtg gattttgaca
    16021 atttcacgct ggaaatcgag agcttcgaga tagtgggcaa agcccatcag gttggcttct
    16081 ggcggcagtt tgtactgcgg gtgcccccag tagccattgc ggaagatccc caactgcccg
    16141 ccttcaacga atttcttcag gcggttttgt acgtcgaaga aatagccagg ggatgatttc
    16201 ggccatgagg agaggctttg cgccagttcg gaggttttcc gcgggtcggc tttcagcgca
    16261 tctaacacat cgatccagtc catcccggca agctgataga agtgcaccag atgatcgtgg
    16321 caccagagcg ttgccagcat aatgttgcgg atgatattgg cgttgtccgg cactttaata
    16381 ccgatggcat cttcgatggc gtaaaccgaa gccagagcgt gtacgccagt acagacgccg
    16441 cagatacgtt caacgaacgc ccacgcatcg cgcgggtcgc gcccttgcag gatgatctcc
    16501 agcccgcgaa acatggtgcc gcaggagacg gcattggtga tcacattctg atcgttaata
    16561 ttcacttcgc agcgcatgtg gccttcaatg cgcgtaatcg ggtcgaccac caggcggcgt
    16621 ccggcattgt tgatggtgta tccctgagtt tcgtactgag tgctcatgcc tgtttatcct
    16681 cattgcctgg ctgatgttcg gtttctgtag gttgctggtt atgacgcctg cgctggtcaa
    16741 cggcgctggc gactgcgtgc acaccaacag ccgctgccac cacgccaagc gcggttaaac
    16801 cgacggtgtc ggcggtggaa tgggtaccca tttgtggaat atcgaccacg cggctgtaga
    16861 acgaaccgcg atcccagaaa ccattttccg cacaacccag gcagccgtga cctgactgga
    16921 tcgggaagga aacgccatcg ttccagcgtg tggaggaaca ggcgttatag gtggtaggcc
    16981 ctttgcagcc cattttgtac aggcagtaac ctttgcgggc agcgtcatca tcccaactct
    17041 ggacgaactc tccggcgtcg aagtgagcgc ggcgatagca tttatcgtgg atacgctgac
    17101 catagaacat caacggacgg cccattctgt cgacatctgg caagcgatca aaggtcacca
    17161 tgtaagtaat gatggcgctc atcacatccg ggatcggcgg gcagccaggt actttgataa
    17221 taggtttgtc ggtgatgact ttgtcgatag gcgttgcctg cgtcggattg ggtcgcgcgg
    17281 cctgcacgca gccccaagac gcgcaggttc cccaggcgat aatcgcactg gctccggcag
    17341 cggcacgttt gagtttctca ataaacggtc gaccgctgct gatacagaac atcccctgct
    17401 cgcccagcgg cggattacct tctactgcga ggatatattt gccattgtat tgcgtgatga
    17461 tgtcttcaaa gacttcttcc gcctgggttc cggcggcagc catcaaagta tcgtcgtaat
    17521 cgagggaaat cagggaaagg atgacgtcct ttgccagcgg gtgagcggag cggataaaag
    17581 attcggtaca gcaggtgcat tccagaccgt ggatccatac caccggaatg cgcggtttgt
    17641 tctccagcgc ccaggcaatc tttggtgcca tccccgcgcc taatcccagc gacgtggcag
    17701 ccagactgca atatttgaga aagctgcgtc gggtaacgcc ctgacgccgc atggcctggt
    17761 aaaatgtttc ctcgttattc atatcgcacg tctcttctcc ttgcgacacc ggcaggacac
    17821 cgggcgcaca tggtcggcaa gcgacgtccc tgtagcaagg caaaccatcg cttcacgtga
    17881 gatgctgaaa acgaaagctc atccttctgc actggcgcac gtcgccagaa agtattgtta
    17941 ataaagcgta gtgaaacttt tgcacaaaac aatacaaact gtgtggattt atcttttagc
    18001 gataaaaatg gacctatttt tctttggccg ggcagtgggg atgtttagcc ggttgccaaa
    18061 cgagtaaaag agaaggaatt catggtgatg aaatgatcaa tctgctgtta tttcacgcta
    18121 tcaatgcact tttggctgtt tttcaggcaa acaaacaaat taggggttta cacgccgcat
    18181 cgggatgttt atagtgcgcg tcattccgga agtgtggccg agcggttgaa ggcaccggtc
    18241 ttgaaaaccg gcgacccgaa agggttccag agttcgaatc tctgcgcttc cgccaaataa
    18301 gataaggggt tagctaaatg ctaacccctt tttcttttgc ctgtcgaaat tctcagggcg
    18361 ttatatttgc ttaatgacct gataatccgc tgttaaacct gttccagatc aaatgcgtaa
    18421 agatgggtaa aacttctgtg taccttccca cattatcatt atgctgctta attaattaca
    18481 tctgtcatag agagtgactc aatggatcgt atagttagtt cttcacatga ccgtacatcg
    18541 ctgcttagca cccataaggt gctgcgtaat acctattttc tgctgagcct gacgctggcc
    18601 ttttcggcga ttaccgcaac tgccagtacg gtgctgatgc tgccatctcc gggtctgatt
    18661 ctgacgctgg tgggtatgta tggtttgatg ttcctgacct ataaaacggc gaataagccg
    18721 accgggatta tctccgcatt cgcctttacc ggttttctgg gttatatcct cggaccaatt
    18781 ctgaatacct atctgtctgc cggaatgggt gacgtaatcg ctatggcact gggcggaacg
    18841 gcgttagtgt tcttctgctg ctccgcatat gtgctgacca cccgcaaaga tatgtcgttc
    18901 ctcggcggta tgctgatggc gggtattgtg gtggtgctga ttggtatggt tgctaatatc
    18961 ttcctgcaac tgcctgctct gcatctggcg atcagcgcgg tcttcattct gatctcctct
    19021 ggcgctattt tgtttgaaac cagcaacatc attcatggcg gtgaaacgaa ctatattcgt
    19081 gccacggtta gcctgtatgt ttcgctgtac aacatcttcg tcagcctgct gagcattctg
    19141 ggcttcgcca gccgcgatta atctcgccca gggttactta ccgagcctcg ctttatgcgg
    19201 ggctttgttt tttgttacac tgccgccagt ctgattaagg tagtaaagaa ttatgctgat
    19261 cttcgaaggt aaagagatag aaacggatac cgaaggctat ctcaaagaaa gcagccagtg
    19321 gagtgaacca ctggcggtgg tgattgcaga gaacgaaggg attgcgctgt cgccagaaca
    19381 ctgggaagtg gtgcgttttg tgcgtgattt ctatctggaa ttcaatactt ctccggcgat
    19441 tcgtatgctg gtaaaagcga tggcgaataa atttggcgaa gaaaaaggta acagccgcta
    19501 tttataccga ctgtttccga aaggtccggc aaagcaagcc accaaaattg ctggcctgcc
    19561 taaaccggta aaatgcattt aacggatacg aaaatccgtt aactccccgg agggatgatg
    19621 cggctcgcta agcacccgtt ccacacgcgc tgaacgcggg ccgccacttt ttaaccactg
    19681 tattaatttt tccacctgcc cttcgtcacc gcaggcaacc acttccacgc tgccatcgtc
    19741 aagatttttg gcgtacccgg ttagtcccag ttttttcgct tcgtactgcg tggtgtagcg
    19801 gaatcctacg ccctgaactc gcccgtaaat ccaggcaatt atgcagactt tcgacattgc
    19861 tgctctcctt atccttaatg gacgcgttgc aaatccatgg aaaactccgg acaatgacgc
    19921 ccatttcttt gcatcgacag aaaagtaaat tatgagtgta cgtttagtgt tagccaaagg
    19981 gcgcgaaaaa tcattacttc gtcgccatcc gtgggtcttt tccggggccg ttgcccgcat
    20041 ggaaggtaaa gccagcctcg gtgaaaccat cgatattgtt gatcatcagg gaaaatggtt
    20101 agcacgcggc gcttattcgc cagcttcgca aatccgggcg cgcgtctgga cgtttgaccc
    20161 gtctgagtct atcgacattg ctttttttac ccgccgttta cagcaagcac aaaaatggcg
    20221 tgactggctg gcgcaaaaag atggcctcga cagctatcgt ttaatcgccg gagaatctga
    20281 tggcctgccg ggtattacta tcgatcgttt cggtaatttt ctggtgctgc aactgctgag
    20341 tgctggcgca gagtatcagc gcgcggcatt aattagtgcc ctgcaaacgc tgtacccgga
    20401 atgtgctatt tacgatcgca gcgacgtcgc ggtacgtaaa aaagaaggga tggagctgac
    20461 tcagggcccc atcaccggcg agttgccgcc tgccctgctg ccgattgaag aacacggcat
    20521 gaaactgctg gtggatattc agcacggaca caaaacgggc tactacctgg accagcgtga
    20581 cagccgcctg gctacccgcc gctacgtcga aaataaacgc gtacttaact gtttttccta
    20641 taccggtggt ttcgccgtat cggcattgat gggcggttgc agccaggttg tcagcgttga
    20701 tacctcccac gaagcgctgg atattgcacg gcagaacgtt gagctgaaca aactggatct
    20761 gagcaaggca gagtttgtcc gtgatgatgt ctttaaattg ctgcgtactt atcgcgatcg
    20821 cggtgaaaaa tttgatgtta tcgtgatgga cccgccgaag tttgttgaga ataaaagcca
    20881 gttgatgggc gcgtgtcgtg gttataaaga catcaacatg ctggcgattc agttgctgaa
    20941 tgaaggcggt attctcctga ctttctcctg ttccggtctg atgaccagcg atttatttca
    21001 gaaaatcatc gcggatgccg caattgatgc cggtcgtgat gtacaattta tagagcagtt
    21061 ccgtcaggca gccgatcatc cggtgatcgc tacctacccg gaagggctat atctgaaagg
    21121 gtttgcctgt cgcgtcatgt aacttgaaat gtggaatttt acccttatat caaaggtaaa
    21181 gatttcccgg gaggtgacta tgattgccag caaattcggt atcggccagc aggtccgcca
    21241 ttccctgtta ggttatctcg gagtggtcgt ggatatcgac ccggtttatt cgctctctga
    21301 accgtcgcct gatgagctgg cggtgaatga cgagcttcgc gccgctccgt ggtatcacgt
    21361 ggtgatggag gatgataacg gcctaccggt tcatacctac ctggctgaag cccagctaag
    21421 tagcgaattg caggatgaac atcctgaaca gccatccatg gatgagctgg cgcagaccat
    21481 ccgcaaacaa ctccaggccc cgcgtctgcg taactaatcc ctgaacaaat ccccggaagg
    21541 tttttgcctt ccggggattt tttatttggc caggcccagg cgagggatct caatcgccgg
    21601 gcagcgatcc atcaccacgt ttagccctgc atcacgcgcc agcactgccg cttgctcatt
    21661 aatcaccccc agttgcatcc agagggtttt cgcgccaatg gcgatagctt cctgcgccac
    21721 gccccacgcc gcttctgaat tacggaaaac atcgaccata tcgacttttt ccggaacgtc
    21781 tgccagcgtg ccatacccct gctggcccag cagtgttttg ccggcaactt ttggcgaaac
    21841 aggaatgacg tgatacccct ggtcgagtag atatttcatc acgcgatagc tgggacgatc
    21901 gggtttgtcg cttgccccca ccagcgcaat ggtgtgagtg gacgttaaaa tgcctgcaat
    21961 atcggtttct ttcattattt cctccagact gtttaacaaa gtgtacgaca aagccgacat
    22021 gccaaccatg cagctaatac gaatgaatga gagcaaaagc gggaaatatg tctatatgtt
    22081 agtaagtatg attaaagaaa aattttgaca cattattatt cgggcggtct tttaacagga
    22141 gctatctatg aaaaccggca tcgtgaccac cttgattgca ttatgtttgc cggtatccgt
    22201 ttttgccacc acgctgcggc tttctactga tgtcgatctt ctggtactcg acggtaagaa
    22261 ggtttccagc tctctgctgc gcggtgcgga cagcattgaa ctggacaatg gaccgcatca
    22321 gttagtgttt cgcgttgaga agacgattca tctttccaat agtgaagaac ggctctatat
    22381 ctcccctcca ctggtggtta gttttaatac ccagctcatc aaccaggtca attttcgcct
    22441 gcctcgcctg gagaatgagc gggaagctaa ccattttgat gccgcgccgc gccttgaatt
    22501 gttggatggc gatgcgacgc cgattccggt aaagctggat attctcgcca ttacctcaac
    22561 tgcaaaaacg attgattatg aggtcgaggt tgaacgctat aacaaatccg cgaaacgcgc
    22621 ttcactaccg caatttgcca cgatgatggc agatgacagt acgctgcttt cgggtgtttc
    22681 cgagctggat gctattccgc cgcagtctca ggtgctcaca gaacaacggc tgaagtattg
    22741 gtttaaactg gctgacccac aaacgcgaaa tactttcctg caatgggcgg aaaaacaacc
    22801 atcctcctga gatttttgtg cctgtgcgca ggctttttca gtctttatct tgcagcgata
    22861 agtacttaca gtaatctgta ggaaagttaa ctacggatgt acattatgga actgacgact
    22921 cgcactttac cttcgcggaa acatattgcg ctggtggcac acgatcactg caaacaaatg
    22981 ctgatgagct gggtggaacg gcatcaaccg ttactggaac aacacgtact gtatgcaaca
    23041 ggcactaccg gtaacttaat ttcccgcgcg accggcatga acgtcaacgc gatgttgagt
    23101 ggcccaatgg ggggtgacca gcaggttggc gcattgatct cagaagggaa aattgatgta
    23161 ttgattttct tctgggaccc actaaatgcc gtgccgcacg atcctgacgt gaaagccttg
    23221 ctgcgtctgg caacggtatg gaacattccg gtcgccacca acgtggcaac ggcagacttc
    23281 attatccagt caccgcattt taacgacgcg gtcgatattc tgatccccga ttatcagcgt
    23341 tatctcgcag accgtctgaa gtaatattgc acaggtggca aacgccacct gtttcttacg
    23401 gttttctcgc caccggcaca tccagatttt tcagtatatc cacaaaggga gagggattct
    23461 ctttgttaaa cagtgcccat acccgatggc gtgcccgggt cagcgccacg tacattaacc
    23521 gccgttcttc agcgtccggg aaatcctcaa ccggtggcag tagcgcctct tccataatcg
    23581 actcccgcgc cgcagccgga aaaccatcac tcccctcctg caagccaacg atgatgacgt
    23641 aatccgcctg ttgccctttg ctggcatgaa tggtcataaa gtcgatttgc aacttcggcc
    23701 agcgtgttgc tgctttttcc aggctggcag gcctcatgtg atggtaacgc gccaggatca
    23761 gaatgcgctc ttccggtttg gcataaccag agagtttatc cagcaaagcg tcaagttgac
    23821 cctcatccaa taacgtgacg gcttttttgt ctccattggt taagctgttt agcggctttt
    23881 taagctggcc tgggttctgc tgaataaacc tgtttgccac ctcaccgata cgactgttaa
    23941 aacggtaggt cgtgtctaaa tcacagcgat caccttcacc aaagttttca tggaaagccg
    24001 tggtaagcga catttgcgca ccgctgaatc ggtaaatcgc ctgccagtca tcaccaacag
    24061 cgaacaacgt cgtttgacta ttttgcttgc gtaatgccgc taacaacgct gcacgctgcg
    24121 gcgagatatc ctgaaattca tcaaccagaa tatgcttcca cgggctgata aagcgacctt
    24181 tctccagaat cacaatcgcc tggtgaataa ggcccgaaaa atcgacagcg ttttctgcct
    24241 tcagcgcacc tttccaggct tttaataacg gggccatcaa cttgatacgt ttactgaaca
    24301 gatcgcgaat ctcttcgggt gcactggcaa tcatttctgc ctgtgcacca ccgtgcatcc
    24361 gcatcagact tacccagcga tcgaggcgag aggcaagacg acgctgtaat ttttcatcat
    24421 cccagaaatt accttctggc actgaccact gcatttcttc cgtcagccat tgccgccagc
    24481 ccttcgcctg cgctttcttt tcgctgcatt gcttgcgcca ctcagcaata aaaagttcat
    24541 gacgggcagc ggtatcattt tccagtttgc tgactatcgg aacttttttg ctgccctgct
    24601 gaataatatg cagcgccagc gcatgaaacg tgcgtgcggt aatgtcttcg gtatgtagtc
    24661 gttctcgaat acgctcgtcc atctcttcag cggctttgcg accaaacgcc agcaataaaa
    24721 tttgctcagg ggacgcttca ccacgcgcca gtaaccagcc agcacgggcc accagcaccg
    24781 acgtttttcc gcttcctgca cctgccagca ctaacagaga atgctcgcca ttaacgactg
    24841 cccgggcctg cgccggattc agcagtgaag actcgacctg gcggaaaaaa tccgcatact
    24901 cggtaagcat ggcttcggta tacgcctggt tatgctgcaa ccgagcgcct tcaatgtcct
    24961 tcaaccaggc ctgacattta cgccacgcct cacggcagtt atcgaattct tccagtcggt
    25021 taaccggcaa tggtaacgcc gacaaagcct ggcggatttg ttgctgcacg ccagaggttt
    25081 gctcacgcgt cagccattta ttttctccgg tgcgcgtggc aatcaaatcc agttgctgac
    25141 gtaaaacgcc agacgcaatt tcgctcatct cgccactcca ccgccgccag tgagcatcaa
    25201 gatgatggta aaaacgctgc gtctcgcccc attcggtgcc gtgcagacgc accacttttt
    25261 cgtccggcag tacaaattcc agctcgcccc ataccagacc gcgcttacag tgaatcgcca
    25321 gtaattgatt gaaaggaata aggtattcgt ggcgatcgcc ggagacttta atcccggcat
    25381 tgaggatcac cgcccgatcg taagggtgct gtgccagacg ttttccaagc gttgtcgctt
    25441 tcagttccat gactctgtag atccacacga aattatttct tatcagtgta accttcagag
    25501 aaaacgtgct ccagcccaaa aaaacgttac aattgccgcc cattactgaa aaccacagta
    25561 aagcgaggtt ttatgcgtac cgttttgaac attctgaact ttgtgcttgg cggatttgcc
    25621 accactctgg gctggctgtt ggcgactctg gtcagtattg tgctgatttt taccttaccg
    25681 ctgacacgat cctgctggga gatcaccaaa ctgtctctgg tgccttatgg caatgaagct
    25741 attcatgtcg atgaactgaa cccggctggc aaaaatgtgc tgctaaatac tggcggtacg
    25801 gtattgaata ttttctggct gattttcttt ggctggtggt tatgcctgat gcacgttgca
    25861 acgggcatcg cacaatgtat ttccatcatt ggcattcctg tcggtattgc gaactttaaa
    25921 attgccgcta ttgcactatg gccggtcggt cgtcgcgtgg tatcggtaga aacagcgcaa
    25981 gctgcgcgcg aagccaatgc acgtcgtcgt ttccaataat ccactaatat ggcctttatg
    26041 ctaagtcctt tgctcaaacg ctatacctgg aacagcgcct ggctgtatta cgcgcgtatt
    26101 tttattgcgc tttgtggaac cacagcgttt ccgtggtggc tgggtgatgt aaaactgacg
    26161 attccgctga cgcttgggat ggtggctgcg gcgctgaccg atctcgatga ccgactggcg
    26221 ggacgtttgc gtaacctcat cattacgctg ttctgctttt ttatcgcctc ggcctcagta
    26281 gaattgctgt ttccctggcc ctggctattt gcgattggct taacgctctc taccagcggc
    26341 ttcattttgc tcggcggtct gggtcaacgc tatgcaacaa ttgccttcgg tgcattgctg
    26401 atcgccattt acactatgtt gggaacatca ctgtatgagc actggtatca gcagccgatg
    26461 tatcttctgg ccggtgccgt ctggtacaac gtcctgacac ttattggtca tctgctgttc
    26521 ccggtccgcc cgctgcagga caacctggcg cgttgctatg aacaactggc gcgttatctt
    26581 gagctcaagt cgcgcatgtt tgatcctgat attgaagatg aaagccaggc accgctgtac
    26641 gatttggctc tcgccaacgg tcagctgatg gcgacattga atcagacgaa actctcgctg
    26701 ctgacccgct tacgtggcga tcgtggtcaa cggggaacgc gtcgcacact gcattattac
    26761 tttgtcgcac aggatattca cgagcgtgcc agctcttctc atattcaata tcaaacattg
    26821 cgtgaacatt ttcgccacag cgacgtgctg ttccgttttc agcggctgat gtcgatgcag
    26881 ggccaggcgt gccagcaact gtcacgctgt attttgctgc gtcagcctta tcaacatgat
    26941 ccgcattttg agcgcgcttt tacgcatatt gatgctgcgc tggagcggat gcgcgataac
    27001 ggcgctcctg ctgatttact caaaacactg ggatttttac tgaacaattt acgtgccatt
    27061 gatgcccaac tggcaacaat tgaatcagaa caggcccagg cactgcccca taataatgac
    27121 gaaaatgagc tcgctgatga cagcccgcac ggattgagtg atatctggct gcgtcttagc
    27181 cgtcacttca cgccggaatc cgccctcttc cgtcatgcgg taagaatgtc gctggtgttg
    27241 tgcttcggct acgccatcat tcagataacc ggaatgcatc acgggtattg gatcttgctg
    27301 acaagtttgt ttgtctgcca gccaaactat aacgccacgc gccaccgcct gaagttaagg
    27361 attattggta cgctggtagg tatcgcaatt ggtattcctg tgctgtggtt tgtaccgtca
    27421 ctggaagggc aactggtgct actggttatt accggcgtgc tcttttttgc cttccgtaac
    27481 gtgcaatacg cccatgcaac gatgttcatc acacttttgg tgctactttg ttttaacttg
    27541 ctgggtgaag gttttgaagt ggcgttacct cgcgtaatcg atacgctgat tggttgtgcc
    27601 attgcgtggg cggcggtgag ctacatctgg cctgactgga agtttcgcaa tctgccgcgc
    27661 atgctcgaac gtgccaccga ggccaactgt cgctatctcg atgccatact ggagcaatac
    27721 catcaggggc gtgataaccg tctggcgtat cgtattgccc gccgcgatgc acacaaccgt
    27781 gatgctgaac tggcgtcggt ggtatcaaat atgtccagcg agccgaacgt taccccgcaa
    27841 attcgcgagg ccgcgtttcg gttgctgtgc cttaaccata cgtttaccag ctatatctca
    27901 gccctcggtg ctcaccggga gcagttaact aatcctgaaa ttctggcgtt tcttgatgac
    27961 gcagtttgct atgttgatga cgcgctacat catcaacctg ctgatgaaga acgcgtcaat
    28021 caggcattag ctggcctgaa acagcggatg cagcaacttg aaccacgggc agacagcaaa
    28081 gaacctctgg tcgtacaaca agttgggtta ttgattgcat tactgccaga gattggtcgt
    28141 ctgcaacgcc agattactca agttccgcag gaaactcctg tttcggcgta agagagtcag
    28201 cccattctgc aagctcctgg cggcgtgcca ccgggagcgc agcttcatga atgccgataa
    28261 tggccccttc aagcataaac agaatctttt ctgtcaccag actgttttgc tgccgcagtc
    28321 gcaaccaaca catttttgcc ccaagtatac gtaacgcccg tacatcctta atccccacct
    28381 ccccgagaat cgcttccaga tgaaaagaca tattgggcaa atctttcagt ctttcccgaa
    28441 tattgcgcgt gcttttctct ttcagcgctg catcgagaga atatttcgac agacgcacca
    28501 gcttcagttg atttcgccat agactttcat caacccgata gtaattgagg gtaacggatc
    28561 ggccacactt tttatatgtc agccagacag gcggatgttt tacacagtac ggtgcacttt
    28621 gctcacaagc ccgaagatac aactcaccat cagaaaccat cgcaaacacc gtgtcgtcaa
    28681 cggttaggct gtaactacca aacaatgatc ggtattcaat tgtgcccaac gtt
//
LOCUS       DADSZD010000044        28638 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.044, whole genome shotgun sequence.
ACCESSION   DADSZD010000044 DADSZD010000000
VERSION     DADSZD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.044"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..459
                     /gene="agaB"
                     /locus_tag="J1Z28_001719"
     CDS             <1..459
                     /gene="agaB"
                     /locus_tag="J1Z28_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBA7716559.1"
                     /translation="LLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDDIQQKLM
                     GITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDLKDVNVG
                     NMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            498..1301
                     /gene="agaC"
                     /locus_tag="J1Z28_001720"
     CDS             498..1301
                     /gene="agaC"
                     /locus_tag="J1Z28_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBA7716560.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1291..2082
                     /gene="agaD"
                     /locus_tag="J1Z28_001721"
     CDS             1291..2082
                     /gene="agaD"
                     /locus_tag="J1Z28_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBA7716561.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2083..2850
                     /locus_tag="J1Z28_001722"
     CDS             2083..2850
                     /locus_tag="J1Z28_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBA7716562.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     RKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFHSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTADRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHNHFTCVIDEMCRR"
     gene            complement(2900..3763)
                     /gene="rsmI"
                     /locus_tag="J1Z28_001723"
     CDS             complement(2900..3763)
                     /gene="rsmI"
                     /locus_tag="J1Z28_001723"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBA7716563.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQENDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            3828..5864
                     /locus_tag="J1Z28_001724"
     CDS             3828..5864
                     /locus_tag="J1Z28_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HBA7716564.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQSQPQTVDDVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRETT
                     DSGMTTNNPTLQTTPTDDQFTNNGSRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            5822..6217
                     /locus_tag="J1Z28_001725"
     CDS             5822..6217
                     /locus_tag="J1Z28_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBA7716565.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFIEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            6237..6827
                     /gene="diaA"
                     /locus_tag="J1Z28_001726"
     CDS             6237..6827
                     /gene="diaA"
                     /locus_tag="J1Z28_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBA7716566.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            6837..7412
                     /gene="yraP"
                     /locus_tag="J1Z28_001727"
     CDS             6837..7412
                     /gene="yraP"
                     /locus_tag="J1Z28_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBA7716567.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(7525..8565)
                     /locus_tag="J1Z28_001728"
     CDS             complement(7525..8565)
                     /locus_tag="J1Z28_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA7716568.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWDFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPTKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(8638..9273)
                     /locus_tag="J1Z28_001729"
     CDS             complement(8638..9273)
                     /locus_tag="J1Z28_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBA7716569.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            9401..9919
                     /gene="yhbO"
                     /locus_tag="J1Z28_001730"
     CDS             9401..9919
                     /gene="yhbO"
                     /locus_tag="J1Z28_001730"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBA7716570.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEMVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(9899..10342)
                     /locus_tag="J1Z28_001731"
     CDS             complement(9899..10342)
                     /locus_tag="J1Z28_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBA7716571.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            10393..10695
                     /gene="yhbQ"
                     /locus_tag="J1Z28_001732"
     CDS             10393..10695
                     /gene="yhbQ"
                     /locus_tag="J1Z28_001732"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBA7716572.1"
                     /translation="MTHWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLNSLQTPEIKSD"
     gene            complement(10682..11185)
                     /locus_tag="J1Z28_001733"
     CDS             complement(10682..11185)
                     /locus_tag="J1Z28_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7716573.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(11179..11703)
                     /locus_tag="J1Z28_001734"
     CDS             complement(11179..11703)
                     /locus_tag="J1Z28_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBA7716574.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            11912..12907
                     /locus_tag="J1Z28_001735"
     CDS             11912..12907
                     /locus_tag="J1Z28_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA7716575.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVNGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            12916..13794
                     /locus_tag="J1Z28_001736"
     CDS             12916..13794
                     /locus_tag="J1Z28_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBA7716576.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            13875..14882
                     /locus_tag="J1Z28_001737"
     CDS             13875..14882
                     /locus_tag="J1Z28_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBA7716577.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(15000..16244)
                     /gene="mtr"
                     /locus_tag="J1Z28_001738"
     CDS             complement(15000..16244)
                     /gene="mtr"
                     /locus_tag="J1Z28_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBA7716578.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(16398..18287)
                     /locus_tag="J1Z28_001739"
     CDS             complement(16398..18287)
                     /locus_tag="J1Z28_001739"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH family ATP-dependent RNA helicase"
                     /protein_id="HBA7716579.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(18280..18360)
                     /gene="yrbN"
                     /locus_tag="J1Z28_001740"
     CDS             complement(18280..18360)
                     /gene="yrbN"
                     /locus_tag="J1Z28_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBA7716580.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(18467..19351)
                     /gene="nlpI"
                     /locus_tag="J1Z28_001741"
     CDS             complement(18467..19351)
                     /gene="nlpI"
                     /locus_tag="J1Z28_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBA7716581.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(19460..21595)
                     /gene="pnp"
                     /locus_tag="J1Z28_001742"
     CDS             complement(19460..21595)
                     /gene="pnp"
                     /locus_tag="J1Z28_001742"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBA7716582.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(21842..22111)
                     /gene="rpsO"
                     /locus_tag="J1Z28_001743"
     CDS             complement(21842..22111)
                     /gene="rpsO"
                     /locus_tag="J1Z28_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBA7716583.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(22260..23204)
                     /gene="truB"
                     /locus_tag="J1Z28_001744"
     CDS             complement(22260..23204)
                     /gene="truB"
                     /locus_tag="J1Z28_001744"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBA7716584.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(23204..23605)
                     /gene="rbfA"
                     /locus_tag="J1Z28_001745"
     CDS             complement(23204..23605)
                     /gene="rbfA"
                     /locus_tag="J1Z28_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBA7716585.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(23769..26441)
                     /gene="infB"
                     /locus_tag="J1Z28_001746"
     CDS             complement(23769..26441)
                     /gene="infB"
                     /locus_tag="J1Z28_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBA7716586.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(26466..27953)
                     /gene="nusA"
                     /locus_tag="J1Z28_001747"
     CDS             complement(26466..27953)
                     /gene="nusA"
                     /locus_tag="J1Z28_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBA7716587.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(27981..28433)
                     /gene="rimP"
                     /locus_tag="J1Z28_001748"
     CDS             complement(27981..28433)
                     /gene="rimP"
                     /locus_tag="J1Z28_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBA7716588.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
BASE COUNT         6811 a         7855 c         7200 g         6772 t
ORIGIN      
        1 ctcttaaccc gtattgataa ccgtctggtt cacggtcagg ttggcgtgac ctggacatcc
       61 accatcggtg cgaatctgtt agtagtcgtg gatgatgttg tcgctaacga tgatattcaa
      121 cagaaattaa tgggtattac cgcagaaacc tacggctttg gcattcgttt ctttactatc
      181 gaaaaaacca ttaacgtcat cggcaaagct gcaccacatc agaagatctt cctgatttgc
      241 cgtacgccac aaacggtacg taaattggta gaaggtggta ttgacctgaa agatgtcaac
      301 gtcggcaata tgcatttctc ggaagggaaa aagcaaatca gcagtaaagt ttatgtcgat
      361 gaccaggatc tcacggactt acgttttatt aaacaacgtg gcgtgaatgt tttcattcag
      421 gacgtccctg gcgatcaaaa agaacaaatc cctgactaaa tctaaaatcg ccttaatatt
      481 gatttgaggt aataaaaatg catgaaataa ccctactcca gggattatcc ctggcggcgt
      541 tagttttctt tctggggatt gatttttggc tggaagcctt atttttattc cgcccgataa
      601 tcgtttgtac ccttacaggt gctattctcg gtgatattca gactggctta attaccggtg
      661 gcctgacaga gttggctttc gccggattaa cccctgcagg tggtgttcag ccgcccaacc
      721 ctattatggc gggtctgatg accactgtca ttgcatggtc tacgggcgtt gatgccaaaa
      781 cggcaattgg tcttggcctg ccgtttagtt tgttaatgca gtacgtcatt ctgttcttct
      841 attccgcttt ctcattattt atgaccaaag ccgataaatg cgcaaaagag gcggatacgg
      901 cagcgttttc ccgacttaac tggacaacga tgctcatcgt cgcttcagcg tatgcggtga
      961 ttgctttcct ctgtacttac ctggcacaag gggcgatgca ggcgctggtg aaagcgatgc
     1021 ccgcctggct gacccacggc tttgaagtgg ctggcggtat tctgcctgcc gttggttttg
     1081 gcttgctgct gcgcgtgatg ttcaaagcgc aatatatccc ttacctgatc gccggtttcc
     1141 tgttcgtttg ctacatccag gtcagcaacc tgttgccggt tgccgtgctg ggcgcaggct
     1201 ttgcggtgta tgagtttttc aatgcgaaat cccggcagca agcgcaaccg cagcccgttg
     1261 ccagtaaaaa tgaagaagag gactacagca atgggatctg aaatcagtaa aaaagatatc
     1321 acccgtctgg gctttcgttc gtcactgcta caagcaagct ttaactacga aaggatgcag
     1381 gcgggcggtt ttacctgggc gatgttgccg atcctgaaaa agatttataa ggacgacaaa
     1441 ccgggcctaa gcgcggcgat gaaagataac ctcgaattta ttaataccca ccctaatctg
     1501 gtcggattcc tgatggggtt attaatttcg atggaagaaa aaggagaaaa ccgcgacacc
     1561 attaaaggcc tcaaagtggc gctgtttggc ccaatcgccg ggattggcga tgcgattttc
     1621 tggtttacct tgttgccaat tatggcgggg atttgctcat cgtttgccag tcaggggaac
     1681 ctgttgggac cgattttgtt tttcgccgtt tacctgctta tctttttcct gcgcgtcggc
     1741 tggacccacg tcggttattc agtcggcgtg aaggctatcg ataaagtgcg agagaactcg
     1801 cagatgattg ctcgttcggc aaccatcctc gggatcacag taatcggcgg gctgatcgct
     1861 tcgtatgtgc atattaacgt ggtgacatcg tttgccatcg acagtaccca cagcgtcgcg
     1921 ctgcagcagg atttcttcga taaagtcttc ccgaatattt taccgatggc ctacaccctg
     1981 ctgatgtatt acttcctgcg ggtgaaaaaa gcgcatccgg tgctgttaat cggcgtgact
     2041 tttgtgctct ctattgtttg ttctgcattc ggcattttgt aaatggaacg aggcactgcg
     2101 tctggtggtg cctctttact aaaggaattt catcctgtgc aaacccttca acaagttgaa
     2161 aactatacgg cgttaagtga acgtgccagc gaatatttat tggccgtgat ccgtcgcaaa
     2221 ccggatgccg tgatttgcct ggcgaccgga gccacgccat tactgacgta tcattatctg
     2281 gtagaaaaaa tccaccagca gcaggttgat gtcagccagc tcaccttcgt gaagctcgac
     2341 gaatgggtgg atctgccatt aacgatgcct ggtacctgcg aaactttcct tcaacagcat
     2401 atcgtgcagc cgctggggct acgtgaagac cagctcatca gctttcactc cgaagagata
     2461 aatgagacag agtgcgaacg ggtaacgaac ctgattgcgc gcaaaggcgg tctggactta
     2521 tgcgttctcg gattggggaa aaacggtcat cttgggctga acgaaccggg agaaagcctg
     2581 caaccggcct gccatatcag tcaacttgat gccagaacac agcaacatga gatgttaaaa
     2641 accgcggacc gtcccgtgac tcgtgggatc accttaggcc tgaaggatat tctcaatgcc
     2701 cgcgaagttt tgttactggt gactggcgaa ggaaaacagg atgcgacaga acgttttctc
     2761 acggctaaag tctccaccgc tatcccggct tcatttttat ggctgcataa ccatttcaca
     2821 tgcgtgattg atgaaatgtg tcgtcgttaa ctcgccaggt aaaggcgttg tagcgctatt
     2881 cctgctgcga cgcctgtaat cactctccct gctgctccag cgcatactta tacagcgcat
     2941 ttttcttcac gccgtgaatt tctgcggcca gcgccgccgc ttttttcagc ggcagttctg
     3001 cctgtagcag cgccagcgtg cgcagggcat cagcaggcaa gtcattttcc tgcgctttat
     3061 ggccttcgac aatcagcacc atttcgcctt tgcgacggtt ttcatcttct tttacccacg
     3121 ccagcagctc gccaacgggc gcgccgtgaa tggtttccca ggttttggtt agctcacgcg
     3181 ccagaaccac gtaacgggat tcgcctaata ccgcaacaat atcttccagg ctatctaaca
     3241 gacggtgggt agattcataa aaaatcagcg tgcgcggctc cgcttcaata gcttttagcg
     3301 catcacggcg gccttttgat ttggcaggta aaaagccttc ataacaaaaa cgatcggatg
     3361 gtaaacccgc ggcgcttaac gcagtgatag cagcacacgg ccctggtaac ggcaccacgc
     3421 gaatccccgc ttcacgacag gtacgcacca gatggtagcc aggatcgtta attagcggcg
     3481 ttccggcatc ggaaaccagc gcaatatttt gcccctcttg cagcttcgcc agcagcgttt
     3541 cggctttttg ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat
     3601 gttgcagcaa taaaccggtg tgacgcgtat cctcggcggc aatcagatca acggcctgta
     3661 atacctctaa tgcacgctgg gtgatatccg ccagattgcc gattggcgtc ggtacaatgt
     3721 aaagctgccc ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc
     3781 cgatttaata ttgagcattg cgtaaaaaaa atatcactgg atacattatg gtaccctcaa
     3841 cattttctcg tttgaaagcc gcgcgttgtc tgcctgttgt tttggcagcc ctgattttcg
     3901 ccggttgtgg cacccatact cccgatcagt ccactgctta tatgcagggc acggcgcagg
     3961 ctgattctgc cttttacctg caacagatgc agcaaagttc tgatgatacc aggatcaact
     4021 ggcaattact cgccattcgt gcactggtga aagaaggtaa aaccggacag gcggtggagt
     4081 tgtttaacca actaccgcaa gaactgaacg attctcagcg tcgcgagaaa acactgctgg
     4141 cggcagagat taaactggcg cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa
     4201 tcacacctgc cgatttagaa caaaaccagc aagcccgtta ctggcaggca aaaatcgatg
     4261 ccagccaggg gcgtccttcc attgatttac tgcgcgcgtt aattgctcag gaaccgctgc
     4321 tcggtgcgaa agaaaagcag cagaatattg acgccacatg gcaggcgctc tcctccatga
     4381 ctcaggagca ggcgaatacg ctggtgatca acgccgacga aaatattctg caaggctggc
     4441 tggatttgca gcgcgtctgg tttgataacc gtaacgatcc tgacatgatg aaagctggga
     4501 tcgccgactg gcagaaacgt tatccgaaca acccgggcgc gaaaatgctg ccaacgcagt
     4561 tggttaacgt aaaagcgttt aaaccagcat cgaccaacaa aatcgccctg ctgttgccgc
     4621 tgaatggcca ggcagcggta tttggtcgca ctattcagca aggctttgaa gcggcgaaaa
     4681 atatcggcac tcagccagtg gcggcccagg tagctgccgc acctgccgca gacgtagcag
     4741 aacaatctca gccgcaaacc gtggatgacg ttgccagccc ggcacaagcc tcggttagcg
     4801 atctgaccgg tgaacagcct gcggcccagc cggtgcctgt aagcgccccg gcgacaagca
     4861 ccgcagcggt aagcgcaccc gcaaatccat ccgcagagct gaaaatctac gatacctcat
     4921 cacaaccact tagccagatc ttaagccagg ttcagcagga tggcgcgagt attgtggtcg
     4981 gtccgttgct gaaaaataac gttgaagagt tgctgaagag caacactccg ctgaacgtgc
     5041 tggcactgaa ccagccggag aacatcgaaa accgcgtcaa tatttgttac ttcgcgcttt
     5101 caccggaaga cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc
     5161 cgctggtgct gatcccacgc agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag
     5221 agtggcagaa actgggcggc ggcaccgttc tgcaacaaaa atttggttcc accagcgagt
     5281 tacgcgcggg tgttaacggc ggttcgggta tcgctttaac gggtagtccg attactccca
     5341 gagaaacaac cgactccggc atgacgacca acaatccaac gctgcaaacc acgccaaccg
     5401 atgatcagtt caccaataat ggcagtcgtg tcgatgcggt gtacattgtg gcaacgccgg
     5461 gtgaaatcgc ttttatcaaa ccaatgatcg ccatgcgtaa cggtagccag agcggtgcaa
     5521 cgctgtacgc cagctcccgc agtgcgcaag ggaccgctgg cccggatttc cgcctggaga
     5581 tggaaggctt gcagtacagc gaaatcccga tgctggcagg cggtaatctg ccgttaatgc
     5641 agcaggcgct cagcgcggtg aacaacgatt attcgctggc tcgcatgtat gcgatgggcg
     5701 tcgatgcctg gtcgttggca aatcatttct cacaaatgcg ccaggttcag ggttttgaaa
     5761 tcaacggtaa taccggaagc ctgacggcaa acccggattg cgtgattaac aggaagttat
     5821 catggctaca gtaccaacaa ggtcaggtag tccccgccag ttaaccacca aacagaccgg
     5881 cgatgcgtgg gaagcacaag cgcgtcgctg gctggaaggc aaaggactac ggtttatcgc
     5941 cgctaacgtg aacgagcgtg gcggcgagat cgatctgata atgcgtgaag gccggaccac
     6001 cgtttttatc gaggtgcgtt accgccgctc tgcgctttat ggcggcgcgg cagccagtgt
     6061 gacccgcagc aaacaacaca aattattaca gactgcccgc ttgtggctcg cgcgtcataa
     6121 tgggagtttt gatactgtgg attgccggtt cgatgtggta gccttcaccg ggaatgaggt
     6181 tgagtggatt aaggatgcct ttaatgacca ctcataatta aggtttaagg attagcgtgc
     6241 aagaaagaat taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg
     6301 cgcttccgga tgccatctcc cgtgcagcca tgacgctggt tcagtctctg ctcaatggca
     6361 acaaaatcct ctgttgtggt aatggaactt ccgctgccaa tgcacagcat tttgctgcca
     6421 gcatgatcaa ccgtttcgaa acggagcggc ccagcttacc tgccattgca ctaaatactg
     6481 ataatgttgt cttaacggca attgccaacg atcgcttaca tgatgaagtg tatgcaaaac
     6541 aggtgcgggc gctgggtcat gcgggagatg tattgttagc catttccacc cgtggcaaca
     6601 gccgcgatat tgtaaaagcg gtagaagctg ccgttacgcg tgatatgacc attgtggcat
     6661 taaccggcta tgacggcggt gaacttgcag gtttgttagg gccacaggat gtggagatcc
     6721 gcattccttc gcatcgtagt gctcgcattc aggaaatgca tatgctgacg gtaaattgcc
     6781 tgtgcgatct gatcgataac acgcttttcc ctcaccagga tgtttaagga gaatacatga
     6841 aggcattatc gccaatcgca gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg
     6901 ctgccgtagt gggtactgct gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg
     6961 gcacccaggt agacgatggt accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg
     7021 aacaaattaa gaaagaagcg cgcattaatg taacggccta tcagggcaaa gtgctgctgg
     7081 ttgggcagtc accaaacgct gaactttcgg ctcgcgccaa acagattgct atgggcgtag
     7141 acggtgccaa cgaagtgtat aacgagattc gtcagggcca gccgattggt ctgggcgaag
     7201 cgtctaacga tacgtggatc accaccaaag tacgttcgca gctgttaacc agcgacctgg
     7261 tgaaatcgtc caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg atggggctgg
     7321 tgactgaacg tgaagcgaaa gcggcggcag atattgccag ccgggtaagc ggcgtgaagc
     7381 gggtaactac ggcgtttacg tttattaaat agcagccctt gtaatgcctg atgcgacgct
     7441 tgcagcgtct tatcaggcct acaacgatgc aaaccgtagg tcggataagg cgtttacgcc
     7501 gcatccgaca tgttttccct caaatcaaac caacagcgcc agcccgccga caatcacacc
     7561 agacactgcc accatcgccc ccgtcagcca taaggctttc gtcgggaacg ctttgcgcag
     7621 cataatcagt gacggcaaac tcaccgccgg gagcgtcatc aacaatgcca gcgccggagc
     7681 agttcccata cctgccagca tcatcgtttg tacaatcgga atttctgctg ccgtgggaat
     7741 gacaaacaag catcctgcca ccgccatcgc caccacccac atcaggctgt tatcgacagc
     7801 accatcggca tgggggaata accagacgcg agcggcaccc aacaccagta ctgcaaggat
     7861 gtaaaccggg atcgtactcc agaaaagcgt ccatagcgcc ctgccccagc ggctaaaaaa
     7921 tcctccctgt gcttccggta tgtcaatttc gaccggtgcc tgcgtttgcg gtgtttcacg
     7981 cacccatttt tgcaccagcg tcgcaatcag caacaccatc accagccccg ccaccagacg
     8041 aatcgccgca aaatcccagc cgaggacgaa ccccataaac accagcgtcg ccgggtttaa
     8101 caacggattc cccatccaga atgccagcgc accgcccatc gacacctgtt ggcgacgcat
     8161 tcccgccgcg accggagccg cacagcaggt acacatcatg cccggcagag aaaacagcgt
     8221 tcccagcaac gtgccgcgaa agcgcgattg cccaagcgta cgcaacaacc agtcacgcgg
     8281 gatcagcacc tgaatcaacg aaccgaggat cacccccagc accgccgctt tccataccgc
     8341 gagaaaatag atcatcgcgt aatccaacgc cgcctgccac ggattagcat ctgcctgcgc
     8401 aaggatagat ttaccgatac tgtgggtttc ggcagcagta aacgctttgc cgtagtaagg
     8461 ttgccatttc acataccaga gaccggcaat gacaacgaga aagaaaagcg cgggtttcca
     8521 ccactgaatg ggcgttgccg cctgagatga agactgacca gtcatagcat tccccaggaa
     8581 taagttatga taattgagcg cgtgaatatt acgctcacta tcaattcttg ggaataatta
     8641 ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc acaccttcat gctcaggccg
     8701 catcgcctct gccagcatga cccgcgccac gtctctcgca tcaatggatt tccagttacc
     8761 gggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc atccgctgtt tgctacgatc
     8821 acccagtaac atcgacgggc gagcaatggt caatttcggc cagttctggg cgattaatgc
     8881 ttcttccatc tcccctttga cgcggttata gaaaaacggc gagtgggcat tagcacccat
     8941 agcactgacc accagcatat gctgcgcacc gagccgccgc ccggttaatg cggtatccac
     9001 caccaacgtg taatcggcat gaataaacgc ctctttgctc cccgcttctc gccgcgtggt
     9061 gccgagacaa caaaacacaa tgtcgatggg atcggtgacc tgcgccagcg cgtcagtcag
     9121 ttgcggatca tggggattaa acacccctgg catatcgccc aacgggcgtc gcgtcggcgc
     9181 agcaatggcg ttaactttcg gttcattaat caacatccgc agtaggtgac cgcccaccag
     9241 ccccgttgcg cctgtaatca gtacctgact catcttcgct cctttacaga attgtctgcc
     9301 ttgcgctccc cggctcatgc accaggctta atagaccatg aggtaattat ccccgattgt
     9361 ggaaaattcg cctcatccaa tgcaacaacg cggaggaagc atgagtaaga aaattgccgt
     9421 tttaatcact gatgaatttg aggattcaga atttacttca cccgcagacg agttccgtaa
     9481 agccggacac gaagtgatta ccattgaaaa gcaagcgggt aaaacggtga aaggcaaaaa
     9541 aggagaagcc agtgtgacca tcgataaatc catcgatgaa gtgacgccag cggagtttga
     9601 tgccctgctg ctaccgggcg gccattcacc ggattatctg cgtggcgaca accgttttgt
     9661 cactttcacc cgtgattttg tgaatagcgg caaacctgtt ttcgccatct gtcacggtcc
     9721 gcagttgctg atcagcgccg atgtgattcg ggggcgcaaa ctgactgccg ttaaaccgat
     9781 cattattgat gttaaaaacg cgggcgcgga attttacgat caggaaatgg tggtcgataa
     9841 agatcagctg gttaccagcc ggacaccgga cgatctgcca gcgtttaacc gcgaggcgtt
     9901 acgcctgctc ggtgcctgag ttacgtagcc aaatcatttt tttaccaaag cccagcgtgt
     9961 tgtcggtgaa tttgatttca tcgagccgga tttcccatac cggggccgac agcattctgg
    10021 caaccggaaa gcgacgattg tacgccttgc gtgcgaggtc gctttcctcg ccttccagtc
    10081 tgcggatctc acctttaaac tgcacgccgc gaattaacgc taccgttttc ggctgaccgt
    10141 ttaccgttcc ggcaattgcc gcctgcggcc cgctcatctg cgcatgacgc gttttttctt
    10201 ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag ataaaaagca ttggcgcacc
    10261 agagttcccc ttcctgctgc acacaccagg tgacaacatg ttgttttgcc agccaacggc
    10321 tgatggcggt gagtgtttcc attgctgttc tcttttatac tgtgggcctg accttaacat
    10381 gcgacgcaga cgatgacaca ctggtttctt tacttgatcc gtaccgccga caataagctt
    10441 tataccggga tcaccacgga tgtcgaacgc cgctatcagc agcaccaaag cggcaaaggg
    10501 gcgaaagcac tgcgcgggaa gggagaacta acgctggcgt tttccgcacc agtgggcgat
    10561 cgttcgctgg cgttacgggc agaatatcgc gttaagcaac tgaccaaacg gcaaaaagag
    10621 cgtctggtag cggaaggtgc agtgtttgca gaactgttga acagcctgca aaccccggag
    10681 attaaaagcg attgaagtgc tcgtgatact caaccagacc agttacaccg ttcagcgcgt
    10741 catccgctaa acgatgtacc tggaaggcac tttcagtgcc cggccagcgg caacgcagat
    10801 cgtgatgtgc cgccagttca aagccgaaac ggctgtacag cgccggatcg cccagcgtca
    10861 ccactgcggc atagccgaac tcattaagcg aatcgagtcc ttcatagacc agttggcgtg
    10921 ccagcccctg tccacggtat ttttcatcta ccgccaacgg tgccatgccg acccattgca
    10981 ggtcttcgcc ctgcacatca accggactaa atgccacata gccaatgacc tgaccttcgt
    11041 catctgtcgc caccagcccc agcgtcagaa agccatcttc acgcagatcg tgaaccagct
    11101 tcgcttccgc atcactttcg aatgaacgac gcagcagggc atcaataccc ggcgcatcaa
    11161 tgggaatttc tactcgaatt agcatggttc acctaccgat gtctgtttgg tttcaggcgc
    11221 ggttttcatt cccgcctcaa caaaatccgc cagttgcagc agcatcatgc gtaaggcttt
    11281 cggcatttgc tccagttcaa tggcgtccat caggtttttc acatacagcc ccagctccgt
    11341 atcgccttca atcaccagcc gacgctggaa gaagagcgta tccggatctt gtttacgcgc
    11401 cgcaatcatc agcagatcgc tggcgtcggc actaaaactc acatcagctt gcgcgttctg
    11461 gctaacgacc agtttgccat tcaccaccga ggtaaaccat tgcaggtcaa tatcacgcac
    11521 atgaatactt aaccagcgac cttcaagaaa ctccagctcg ccatcatcca gcgcctgacg
    11581 gaattgccag cttaagacct gctcaagaac ctggcgtttt agcgcaaatg gcgtcagttt
    11641 taccggtaca ctcaacagag atggccccaa atgcacaata cgggaacgca gtttatccaa
    11701 cacgagtttt actccctgtt tcaacaatca tcctattttg ccatatcaga aaaataacat
    11761 agcggtataa atcaacaatt ccatatgaaa ttgctgctac caccaataca actttaactg
    11821 ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc ccagttcgtt aacatttttg
    11881 cgttttgata acgcaacctt caggaaaaat tatggagctg ctctgccctg ccggaaatct
    11941 cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct gtttatatcg ggctaaaaga
    12001 tgataccaat gcccgtcact tcgccggcct taactttacc gagaaaaaat tgcaggaagc
    12061 ggtgagtttt gtccatcaac atcgccgcaa acttcacatc gcgattaaca cttttgcgca
    12121 tccggacggt tacgcccgtt ggcagcgcgc cgtggatatg gcggcgcagc tgggtgccga
    12181 cgcgctgatc ctcgccgacc tagccatgct ggagtacgcc gccgagcgtt atccgcatat
    12241 tgagcgccac gtatcggtgc aggcttcggc gaccaatgaa gaggcgatta acttttatca
    12301 tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg ttgtcgattc atcaggtgaa
    12361 acaactggca cgggtcacac ctgtaccact ggaagtcttt gctttcggca gcctgtgcat
    12421 tatgtcggaa ggtcgttgct atctgtcgtc gtatttgacg ggtgagtcgc ccaacaccgt
    12481 gggcgcgtgt tctccggccc gtttcgtgcg ctggcaacaa acgccgcagg ggctggaatc
    12541 ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc gaaaacgcag gttatccgac
    12601 gctatgtaaa gggcgttatc tggtgaacgg cgagcgctat cacgcgctgg aagaaccaac
    12661 cagtctcaat accctggaac tgctgccgga gttaatggcg gcgaatattg cttcggtgaa
    12721 aattgaaggc cgccagcgta gcccggcgta tgtcagccag gtggcgaaag tctggcgtca
    12781 ggctatcgac cgttgtaagg ccgatccgca aaacttcgta ccgcaaagcg cgtggatgga
    12841 gacgctcggg tcgatgtccg aaggcacgca aactaccctt ggcgcgtatc accgtaaatg
    12901 gcagtgagaa aagcaatgaa atattcctta gggccggtgc tatggtactg gccaaaagag
    12961 acgctggaag aattttatca gcaggccgcc gccagcagcg ccgacgtgat ttatcttggt
    13021 gaagcggtct gtagcaaacg ccgggcaacc aaagttggcg actggctgga gatggcaaaa
    13081 tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc tggcgctggt gcaggcatcc
    13141 tccgaactgg gcgaactgaa acgctatgtt gagaacggtg agtttctgat tgaagccagc
    13201 gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc cgttcgtcgc cgggcacgcg
    13261 ctgaactgct acaacgcggt gacgctgaaa atattgctca aacagggcat gatgcgctgg
    13321 tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc tgcttaatca gtgcgatgag
    13381 ttgggcattc gcaaccagtt tgaagtggaa gtcctgagct acggtcatct acctctggcc
    13441 tactccgccc gctgctttac cgcgcgttcg gaagaccgcc cgaaagatga gtgtgaaacc
    13501 tgctgcatta aatatccgaa cgggcgcaac gtactgtcgc aggaaaacca acaagtgttt
    13561 gtactcaacg gcattcagac catgagcggc tacgtttaca acctcggtaa cgagctggca
    13621 tccatgcagg ggctggtcga tgtggtccgc ctgtcaccgc agggtactga cactttcgca
    13681 atgctcgatg ccttccgcgc taatgaaaat ggcgctgcgc cactaccgtt aacggcaaac
    13741 agcgattgta acggctactg gcggcgactg gccgggctgg agctgcaagc gtaagtatat
    13801 agctcacttt gttaacaact ttaactactc tttaatgcag tattaaagat taatcggtaa
    13861 caaagtgagc tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca
    13921 tccccgaagg ttcttcagcg cgggaagcgt tctcccactc tctcgacctc gcccgtctgg
    13981 ctgaaaagcg cggctatcat cgctactggc tggcggaaca ccacaatatg accggcattg
    14041 ccagtgctgc aacgtcggtg ttaattggct atctggcggc gaataccacc acgctgcatc
    14101 tggggtctgg cggcgtgatg ttgcctaacc actcaccgtt ggtgatcgcc gaacaattcg
    14161 gcacgctcaa tacgctctat ccggggcgaa tcgatttggg gctgggccgc gcgccgggta
    14221 gcgatcagcg aaccatgatg gcgctgcgtc gtcatatgag cggcgatatt gataatttcc
    14281 cccgcgatgt cgcggaactg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc
    14341 gcccggtacc aggctatggc gagaaaatcc ccgtgtggtt gttaggatcc agcctttaca
    14401 gcgcgcaact ggcggcgcag cttggtctgc cgtttgcgtt tgcctcacac ttcgcgccgg
    14461 atatgttgtt ccaggcgctg catctttatc gcagcaactt caaaccgtcg gcacggttgg
    14521 agaaaccgta tgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgatgccg
    14581 aattcctgtt tacctcaatg cagcaagcct ttgtgaaact gcgccgcggc gaaactgggc
    14641 aactgccgcc accgattcaa aatatggatc agttctggtc gccgtccgaa cagtatggtg
    14701 tgcagcaggc gctgagtatg tcgctggtgg gcgataaagc gaaagtgcgt catggcttgc
    14761 agtcgatcct gcgcgaaacc gacgccgatg agattatggt taacgggcag attttcgacc
    14821 accaggcacg cctgcattcg tttgagctgg cgatggatgt gaaggaagag ttgttggggt
    14881 agtgtgtttt aacgtaggaa gccttatccg agatggcaac gctgtcctac gtaggcctga
    14941 taagcgaagc gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat
    15001 tactgataca ccggcagcac attaaagctc gataagatat ggaccagtgc gttgccaacg
    15061 ccaaatacca gaatcagcat aatcatcggc ttgccgcccc atacacggaa tttcgggcta
    15121 ccaaagcgtt tacgcgatgc gcgggccaac agcgccggaa caattgccgc ccagatggta
    15181 gccgctaagc cagcataacc aatggcgtac aggaatccgt tcgggaacag caggccaccc
    15241 acaacaggcg gggcaaaggt cagcaacgct gttttcaaac ggcccatagc cgagtcgtca
    15301 aaaccaaaca gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc
    15361 gctaccgcaa agtttgagaa cacgaccagt agaagatcca gactacggct gttcagtacg
    15421 ccgcttaacg cctgtaccag cacatcaata ttaccgccct tctcggcgat accgataaac
    15481 tccggacgcg ggatgttacc catcgtcgcc agcaaccaga tggtatacag cgccagcgcc
    15541 atcagcgtac cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc
    15601 atcaggctcg gtacgttacc gtgataacca aacgatgcca gacagaacgg cagcgtcatc
    15661 aacagatacg gtgcataaga cgcattgctt tcggcgacgt tgaacaatgt cgcaggctgc
    15721 acatgcccca gcaggctgcc gaaggtgagg aagaaagtaa tgactttcgc ccccagcaca
    15781 attgctgtca tccggctgac agctttcgta ctcaaccaca ccacgaacgc taccagcagt
    15841 gcaaaaccaa atccagctgc ccgtgcagga acgttcagcg acatctccgc gaaggtgtga
    15901 tgcagaatcg aaccgctggc agaaatatag gcgtaagtca ggatatagag cacaaaggca
    15961 atggaaatgc cgttgactac gttccagcct ttgcccagca aatctttggt gatggtgtca
    16021 aagctagaac cgattcgata attcaggtta gcttccagaa tcatcaagcc ggaatgcagc
    16081 atacagaacc aggtaaagat cagcgccgcc attgaccaga agaaccacgc cccggacatg
    16141 accacaggca gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg
    16201 ccaagcagcg acggtgacgt ttgggtggtg gttagtgttg ccatgagggc ttctctccag
    16261 tgaaaaatag tgcgactgtg ttgttatgca ttgcactgta ccagtacatg agtacaaaaa
    16321 acagaaaaaa agccccgatg gttaaaatcg gggctgtata tattatttta cagattgtgt
    16381 tcgctgttca gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca
    16441 cgacgcggtg cgcggccttc acgacgttcg ccgctaaaac gacgcccatc accacgaccc
    16501 ccttcacggc gttcaccgct gaagttacga ccgccttcac gacgttcgcc accgaaacca
    16561 cgaccaccgc cacgacgctc accgccagta tgcggctgtg catcgcccag taactgcatg
    16621 ttcatcggct tgttgagaat gcgagtacgc gtaaagtgtt gcagcacttc acccggcata
    16681 cctttcggca gttcgatggt ggagtgagaa gcaaacagct tgatgttacc gatgtaacgg
    16741 ctgctgatgt cgccttcgtt agcaatcgca ccaacgatat gacgaacttc aacaccatca
    16801 tcgcggccca cttcaatacg gtacaactgc atatcgccaa catcacgacg ttcgcgacgc
    16861 ggacgatctt cacggtcgcc acgcgggcca cggtcgttac gatcgcgcgg accacggtca
    16921 tcacggtcac ggaattcacg tttcggacgc atcggcgcat ctggcggaac aatcagagta
    16981 cgttcaccct gtgccatttt cagtagtgcg gctgccagag tttcgagatc cagctcttca
    17041 ccttcggcag tcggctgaat tttgctcagc agtgcacggt attgatccag atcgctgctt
    17101 tccagctgct gctgtacttt agcggcaaat ttttccagac gacgtttgcc cagcaattct
    17161 gcgttcggca gttctacttc cggaatggtc agcttcatgg tgcgttcaat gttacgcagc
    17221 agacgacgct cacggttctc aacgaacagc agcgcacggc cagcacggcc cgcacgaccg
    17281 gtacgaccga tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaaccacc
    17341 aggctgatac gctcaacgtc caggccacgg gccgcaacgt cggtcgcaat caggatgtcc
    17401 agacgaccat ctttcagtcg ttccagtgtc tgctcacgca gcgcctggtt catgtcaccg
    17461 ttcagcgcgg cgctgttgta gccgttacgc tcaagggctt ctgccacttc cagagtcgcg
    17521 tttttggtac gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc
    17581 agtgcttcgt ttttacgcat accccagaca gtccagtagc tctggctgat gtcaggacgg
    17641 gtagtcacgc tggactgaat gcgcacttcc tgcggctctt tcataaagcg gcgggtaatg
    17701 cgacgaatcg cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc
    17761 tgcgccataa tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca
    17821 tccagaacca gaccgctcaa tttagagagg tccagagtgc cacgtttcag gtggtccagc
    17881 agacggcccg gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg
    17941 tcataacgtt ggccgccgta cagggcaacc acgtttacgc cgcgcatgtg tttagagaaa
    18001 tccgtcattg cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc
    18061 tgtggtgctt tcagctcagg atcaagattc tgcagcagag gtaaagagaa tgctgcagtt
    18121 tttccgctcc ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata
    18181 cactctgcct gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata
    18241 ggagccttca ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc
    18301 ctcaaaatta atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat
    18361 tgaaaagtgt gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt
    18421 aaggtgatgg caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg
    18481 attctgccag gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt
    18541 gctcaacaaa gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt
    18601 ccaaatcccc cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct
    18661 cagcgagcga ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc
    18721 taatgttgcc caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt
    18781 cgaagtgctg tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat
    18841 aaagccacag actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat
    18901 cttgcgctaa cttgtcacga ccgccgtaat ataatgcgat cccgcgattc aagtgcgcgt
    18961 agttgtaagt tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat
    19021 tgcctgcctg cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga
    19081 ttgccagcgc ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca
    19141 acactccgcg ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa
    19201 ggatctgttc catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg
    19261 cgaggacttc acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg
    19321 caacgaaaca ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg
    19381 aacgtcctgt tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag
    19441 cggagggcaa atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag
    19501 caggttgaga ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa
    19561 cttccagaac tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa
    19621 cgcgtttgtc agcgatctga gagatgtgta ccagaccttc tttaccgccg ccgatggcaa
    19681 caaatgcgcc aaagtcaacg atacgggtca ctttaccatt gtagacgcgg cccacttcga
    19741 tttctgcagt gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg
    19801 ctgcgatttt cacagtaccg tcatcttcga tttcgatggt ggtgccagtt tcttcggtca
    19861 gagcacggat aacagaaccg cctttaccga taacgtcttt gatcttgtcc gggttgatct
    19921 tgatggtatg gatacgcgga gcgaactcag agatatcgcc gcgcggcgcg ttgatcgcct
    19981 gttccattac gcccaggata tgcagacgcg cacctttagc ctggttcagt gcaacctgca
    20041 tgatctcttt ggtgatacct tcaattttga tatccatctg caatgcagag ataccgtcgc
    20101 gggaacccgc aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag
    20161 acagtacaac gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag
    20221 ctttgattgg cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag
    20281 aagaagaacc gttggattcg gtgatttcag acactacacg tacggtgtac gggaatttgt
    20341 ccatatccgg catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac
    20401 gacgcttcgg agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt
    20461 ggaacaggaa ggtgtcagta cgttcgccca tcagttcatc aagaacttgc gcgtcacgtg
    20521 cagtacccag cgttgcggta accagcgcct gagtttcacc acgggtgaaa agcgcagaac
    20581 cgtgagtacg cggcagcacg ccagtacgca catccagacc acgaatcata tctttttcac
    20641 gaccgtcgat acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatag
    20701 cgtgcagaat ttcacccagt tcgttttcgt ccagggtttc atcttcagca agcagcgtcg
    20761 cgatggtttc agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc
    20821 ggtaagcatc gctcagacga gcttcagcca gcgcagcaac gcgcgcgttc agcgcttcgt
    20881 ttaccggctc cggctgccag tcccaacgcg gtttaccggc ttctttcacc agttcattga
    20941 tgttctgaat aacaacctgc tgttgttcat gaccgaacac cactgcgccc agcatttggt
    21001 cttcgctcag cagttcagct tcagattcaa ccatcagcac ggcagcttct gtaccggcaa
    21061 caaccaagtc cagtttgctc tctttcagtt cgtcctgagt cgggttcagt acgtactggt
    21121 cattgatgta acctacacgg gcagcaccaa tcgggccatt gaacggaata ccagacagag
    21181 acagggctgc ggaagcacca atcatcgcga cgatatccgg gttaacctgc gggttaacag
    21241 aaaccacggt ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa
    21301 tcgggcggtc aatcagacgc gcgatcaggg tttcgccttc gcttgggcgg ccttcacgac
    21361 ggaagaagct acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca
    21421 gtgggaagaa gtcctgacct ggtttggctt ttttctgacc aacaacggta acaaataccg
    21481 cggtgtcatc catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt
    21541 ccagagtcac ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa
    21601 tatcctttct ctttcttaga cagtacctta cggtactggt gttaataccc gatcttctgc
    21661 gcatcctcgc gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag
    21721 ccgcgcgaac ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa
    21781 ttgctgccgt cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga
    21841 attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt
    21901 tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt
    21961 ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca
    22021 gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga
    22081 ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa
    22141 ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta
    22201 cgcgacgtta agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat
    22261 tacgccgggt attcaaccac caggcgacga ggcgcaacgc ggccttcatc gtcaatttcg
    22321 cccataccga taaacttgcc gttctcaccc tctgtaacgc gaaccagtcc ttccagcggc
    22381 gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga
    22441 ttcaccactg ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac
    22501 tccgcagccg gaatatcctg ctgttcggct tgctcaacca gttcacgcag atgttccagg
    22561 gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaagta aataacatgc
    22621 gcgccacaac cgagtttttc gccgagatcg tcaatgatag tgcggatata agtgcctttt
    22681 gagcagtgaa tttccagctc cagctcattg ccttcatggc gaataaacag caattcataa
    22741 acggtaatcg ggcgcgcttc acgcggaact tcaatgccct gacgcgcgta ttcgtacagt
    22801 tttttgccct gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg
    22861 aaggtatcca gcgccgccgc cagttgctct gcactaaagg ttaccggacg ttcttcaacg
    22921 atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag
    22981 cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc
    23041 aacatgccgg tcgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa
    23101 atccgtttca ccttttgcag cgcatcgttg ctggacatcc cctgcggttt atccagcaac
    23161 aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt
    23221 cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca
    23281 tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca
    23341 ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcctgc aacgctttga
    23401 tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat
    23461 acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc agacgaggat
    23521 ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct
    23581 gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc
    23641 cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca
    23701 gcgtcgcatc aggcaatcca tgcaggatgc ggcataaacg ccttatcccg catggaaccc
    23761 taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc gatcacatcg
    23821 ccagtgcgga catcgttgta gttcttaacg ccgataccac attccatacc gttacggact
    23881 tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg
    23941 ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacaa
    24001 cctgcgatgg caccaaattt cggtgatttg aacacgtcac gaacttccgc cagaccgata
    24061 atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcgatc
    24121 aggttataga tgacggagta gtaacgcaga tccaggcttt ccgcttcaat cactttacgt
    24181 gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg
    24241 gtagcgtcgg tttcggtgat accacctacg ccagaaccaa tgatcttcac tttaacttcg
    24301 tcagtagaca gtttcagcaa ggagtcggaa attgcttcca cagaaccctg tacgtctgcc
    24361 ttcaggacga tattcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg
    24421 agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg acgatagagt
    24481 gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt
    24541 acgccggaca ggccaaggat ttccaccgga atggacggac ccgcttccag cacttcctgc
    24601 cccagttcgt tacgcattgc acgaacacga ccgtattcga agccacacag aacgatatcg
    24661 cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg
    24721 aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga
    24781 acttccgcct gcagcaggat agcatccagc agttcatcaa taccggtacc cgcttttgca
    24841 gatacgtgta cgaactggct ttcgccgccc cactcttctg gcaggatgcc gtactgggag
    24901 agttcgttct taacgcgatc cggatcggct tctggtttat cgattttgtt cacagcaacc
    24961 accaccggaa cacccgccgc tttcgcgtgc tggatagctt cgatggtctg cggcattaca
    25021 ccgtcgtctg cagcaacaac caggactacg atgtccgttg cctgcgcacc acgtgcacgc
    25081 attgaagtaa acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt
    25141 tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggccactttc
    25201 gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc
    25261 acaaccggcg cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct
    25321 tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc aaccagctgt
    25381 gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagctt catcatcgct
    25441 ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac agtgatggtt
    25501 tcgccgatca caacgtcacg gttaacagcc tgagctggct tctggaagcc ttgctgcagc
    25561 gaagaacctt tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga
    25621 tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgcagcttt cgcgttacga
    25681 ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt
    25741 tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat
    25801 ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt
    25861 ttacgacgcg cttcttcttc ggctttacgc ttgagctctg cagcttcctg ctcacggcgg
    25921 gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt
    25981 tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcggcctca
    26041 cgttcagctt tttgttgcgc ctcacgtttg gccgattctt ctgcctcacg acgggcttgc
    26101 tcttccgctt cacgctgcgc ttgctcttcc gcagcaaggc gttcagcctc ttgcggatcg
    26161 cgtttcacaa aggtgcgttt cttgcggact tcgatttgta ccgatttgct ttttccaccg
    26221 gtaccaggaa tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct
    26281 gaatttttct gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg
    26341 tcagcagact tccggatacc tgcatcagca aattgctgta ccaggcgttc cacggaggtc
    26401 tgtcgctctg cggccagcgt tttaatcgtt acatctgtca tgctgttcct tcctgctaca
    26461 gtttattacg cttcgtcacc gaaccagcaa atattacggg cagccataat cagtgctccg
    26521 gctttttcgt cggtcaaccc ttcgatatca gccagatcat caatgccctg ttcggcgaga
    26581 tcttccagcg tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg atctatccct
    26641 tcaaggttca gcagatcgtc agccggtttg ttatcaccga ggctttcttc ctgggcctgt
    26701 gcaatggtgg ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt cggctcatca
    26761 aggccttcga tttccaacag ctctttcatc ggcacatagg ccagttcttc cagcgtcgag
    26821 aagccttctt ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag
    26881 gtgtcgatcg ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc
    26941 acgttgagtt cccaaccgct cagctgcgaa gccagacgca cgttctgacc attacggcca
    27001 atcgcctgcg ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc
    27061 accacgatag aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg cgccgggtta
    27121 tcatcccaca ggacgatatc gatacgctct ccgcccagtt cagtagaaac cgcctgaaca
    27181 cgcgcgccac gcatacctac gcaagcacct accggatcga tacgtttatc gttggttttc
    27241 accgcgattt tcgcacgaga acccggatcg cgagccgctg ctttaatttc aatcacttct
    27301 tcgccgattt ccggcacttc aatacggaac agttcgatca gcatttccgg cttggagcgg
    27361 gtgacgaaca gctgcgcgcc acgcgcttcc gggcgaacgg aatagagcac gccacgaacg
    27421 cggtcgccag ggcggaagtt ttcacgcggc agcatatctt cgcgcaggat cacggcttca
    27481 gcgttgttgc ccagatccag agagatgttg tcgcggttta cttttttcac cacgccggtg
    27541 atgatttcac cttcgtgttc acggaactga tcaaccacca ttgcacgttc ggcttcacgc
    27601 actttctgca cgataacctg ttttgccgtc tgggtagtga tacggtcaaa ggtaacagac
    27661 tcaatctgat cttcaacgta atcgcccagg ttcaggcttt catcttcata acgtgcggct
    27721 tcaagggtga tttccttggt cggctgggtg acttcatcaa caactaacca gcgacggaag
    27781 gtgtcaaaat caccgctttt gcgatcgatc tgtacgcgaa cgtcgatttc ttgttcatat
    27841 tttttctttg ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt
    27901 agcgcctttt cattggatac ggcttcaact acagccaaaa tttctttgtt catcgcgggc
    27961 ttttcacctc atccagacta ttaaaagtgg ggaaccaggt tcgccttctg gatattactc
    28021 agcgcgaaca cttcatcttt accttcgacg gtaactgtga tcatttcacc gtctaccgct
    28081 ttgataacgc cctgccattt acgacggttt tgtaccgcca tacggagaac cagagtcacc
    28141 tcttctccga caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc gagacccggt
    28201 gaggagactt ccaggttata agcaacggtg atggggtctt caacatccag cacagcactt
    28261 acctggtggc tcacatcagc acaatcatca acattgatgc catcttcact atcaatatag
    28321 atgcgcagtg tggatgtgcg accgcgaata aattcgatgc caaccagttc aaaacccagg
    28381 gcctcaactg gcgcagtaat catctctgtt aatttttgct ctaatgtgga caagcccacc
    28441 cccaagacat aaaaaaaggg cctaaagccc agttattctg tagtcagata acaaaaaacc
    28501 ccgataaatc ggggctttat ataactgaac cctataaccg caactgcggt ctggagcact
    28561 ttccagaagg attttttcaa atcccactac gaaggccgaa gtcttcacag tatatttgaa
    28621 aaaggactct aagggaaa
//
LOCUS       DADSZD010000045        28134 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.045, whole genome shotgun sequence.
ACCESSION   DADSZD010000045 DADSZD010000000
VERSION     DADSZD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28134
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.045"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..881)
                     /locus_tag="J1Z28_001749"
     CDS             complement(<1..881)
                     /locus_tag="J1Z28_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716589.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQ"
     gene            complement(959..2182)
                     /locus_tag="J1Z28_001750"
     CDS             complement(959..2182)
                     /locus_tag="J1Z28_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBA7716590.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2208..2597)
                     /locus_tag="J1Z28_001751"
     CDS             complement(2208..2597)
                     /locus_tag="J1Z28_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HBA7716591.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2614..3570)
                     /gene="arcC"
                     /locus_tag="J1Z28_001752"
     CDS             complement(2614..3570)
                     /gene="arcC"
                     /locus_tag="J1Z28_001752"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7716592.1"
                     /translation="MVKPLAVVAVGGNALIQDEQRNSIPDQYVAVMESVQHIVDMVEA
                     GWDLVLTHGNGPQVGFILRRSELASNEVSPVPLDYAVGDTQGAIGYMFQKALHNELAR
                     RGINKPVIALVTQTRVSPHDDAFASPSKPIGAFLDEATAQQRQQQLGWTLMEDAGRGW
                     RRTVPSPAPLEIIEHDTIAHLVRQGYLVIACGGGGIPVVRDGQQLKGVEAVIDKDLAS
                     ALLASQLGADLLVIPTGVEKVAINFGTPQQQWLDAISVAEAQTLLREGQFGVGSMQPK
                     VEAIVDFINASQQQGKQASGLITSPQTIKAALAHQSGTWITL"
     gene            complement(3563..4987)
                     /locus_tag="J1Z28_001753"
     CDS             complement(3563..4987)
                     /locus_tag="J1Z28_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA7716593.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPMLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAITQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSGTSDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGMGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVAKENHHG"
     gene            complement(4984..6543)
                     /gene="fdrA"
                     /locus_tag="J1Z28_001754"
     CDS             complement(4984..6543)
                     /gene="fdrA"
                     /locus_tag="J1Z28_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA7716594.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALVLAQQRLTSKPDDETDSGIKSPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVARTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAANIAVALLNDQPLPAVETEISCADLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTAPASEL
                     LDQLSHIDMNNAPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6638..7498)
                     /locus_tag="J1Z28_001755"
     CDS             complement(6638..7498)
                     /locus_tag="J1Z28_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA7716595.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGRMLTLEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQAPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGHFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7504..8172)
                     /locus_tag="J1Z28_001756"
     CDS             complement(7504..8172)
                     /locus_tag="J1Z28_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBA7716596.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8429..9238)
                     /locus_tag="J1Z28_001757"
     CDS             complement(8429..9238)
                     /locus_tag="J1Z28_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBA7716597.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9500..10261
                     /gene="udp"
                     /locus_tag="J1Z28_001758"
     CDS             9500..10261
                     /gene="udp"
                     /locus_tag="J1Z28_001758"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBA7716598.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            10402..11829
                     /gene="rmuC"
                     /locus_tag="J1Z28_001759"
     CDS             10402..11829
                     /gene="rmuC"
                     /locus_tag="J1Z28_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBA7716599.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKIQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYSR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            11924..12679
                     /gene="ubiE"
                     /locus_tag="J1Z28_001760"
     CDS             11924..12679
                     /gene="ubiE"
                     /locus_tag="J1Z28_001760"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBA7716600.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMEDAGFESVDYYNLTAGVVALHRGYKF"
     gene            12693..13298
                     /gene="ubiJ"
                     /locus_tag="J1Z28_001761"
     CDS             12693..13298
                     /gene="ubiJ"
                     /locus_tag="J1Z28_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBA7716601.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAECDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDSLTKRLEKLEAK"
     gene            13295..14935
                     /gene="ubiB"
                     /locus_tag="J1Z28_001762"
     CDS             13295..14935
                     /gene="ubiB"
                     /locus_tag="J1Z28_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBA7716602.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPNMRITLPLRLWRYTLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     RLAKQQIEAAMGGLPVEAWFDDFDIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            15014..15283
                     /gene="tatA"
                     /locus_tag="J1Z28_001763"
     CDS             15014..15283
                     /gene="tatA"
                     /locus_tag="J1Z28_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBA7716603.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            15287..15802
                     /gene="tatB"
                     /locus_tag="J1Z28_001764"
     CDS             15287..15802
                     /gene="tatB"
                     /locus_tag="J1Z28_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBA7716604.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            15805..16581
                     /gene="tatC"
                     /locus_tag="J1Z28_001765"
     CDS             15805..16581
                     /gene="tatC"
                     /locus_tag="J1Z28_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBA7716605.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAVSEKTEE"
     gene            16623..17405
                     /gene="tatD"
                     /locus_tag="J1Z28_001766"
     CDS             16623..17405
                     /gene="tatD"
                     /locus_tag="J1Z28_001766"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBA7716606.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVACGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(17402..17890)
                     /gene="rfaH"
                     /locus_tag="J1Z28_001767"
     CDS             complement(17402..17890)
                     /gene="rfaH"
                     /locus_tag="J1Z28_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBA7716607.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            18057..19550
                     /gene="ubiD"
                     /locus_tag="J1Z28_001768"
     CDS             18057..19550
                     /gene="ubiD"
                     /locus_tag="J1Z28_001768"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBA7716608.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            19596..20297
                     /gene="fre"
                     /locus_tag="J1Z28_001769"
     CDS             19596..20297
                     /gene="fre"
                     /locus_tag="J1Z28_001769"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBA7716609.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(20580..21743)
                     /gene="fadA"
                     /locus_tag="J1Z28_001770"
     CDS             complement(20580..21743)
                     /gene="fadA"
                     /locus_tag="J1Z28_001770"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBA7716610.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARDLGLKPRACVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(21753..23942)
                     /gene="fadB"
                     /locus_tag="J1Z28_001771"
     CDS             complement(21753..23942)
                     /gene="fadB"
                     /locus_tag="J1Z28_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBA7716611.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAIAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEAAMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLNYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFNANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSHAKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            24132..25463
                     /gene="pepQ"
                     /locus_tag="J1Z28_001772"
     CDS             24132..25463
                     /gene="pepQ"
                     /locus_tag="J1Z28_001772"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBA7716612.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            25463..26077
                     /locus_tag="J1Z28_001773"
     CDS             25463..26077
                     /locus_tag="J1Z28_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBA7716613.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            26116..27567
                     /gene="trkH"
                     /locus_tag="J1Z28_001774"
     CDS             26116..27567
                     /gene="trkH"
                     /locus_tag="J1Z28_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBA7716614.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            27579..28124
                     /gene="hemG"
                     /locus_tag="J1Z28_001775"
     CDS             27579..28124
                     /gene="hemG"
                     /locus_tag="J1Z28_001775"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBA7716615.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPERCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6589 a         7177 c         7553 g         6815 t
ORIGIN      
        1 aattgtgggt ttcttaaata ttctatgcac cactgacagg ctttgcccat accatcggaa
       61 cgagcggcaa gaaataactt ggtggcgtgt ttatgttcgc gcattggctt tttaagcaat
      121 ttaccgctgt tcaataacgg caatgccaga tgatgtggaa tataaccaat accgacgttt
      181 tgttctatta aggcgattgc cgtagcgaag tcaggaacaa aaatgggttt ttgtccttcc
      241 aacaaccagg cttgcatcgg ggggaagtta acggaggtat cgcggataca aaccgctgga
      301 taatgacgca attcactgtt ttctaatgga tgccgcttcg ctgccagtgg gtggagtggg
      361 ctgacgacaa aatcccattc catttgtccg accggttcgc taatgatgcc ttcactgctt
      421 ggtactgcat gaggagcacc gtagaccaga tcagcacgct tactgtacag cgcatcccag
      481 cagccgttgt atacctcaag atctaccgtc agtgttgttg atgtgaattg ccgttcaaaa
      541 tctcgaataa atgccaccag caaatcacca ggaataatat tgtttaccgc aattgtcagt
      601 tcacgctcaa taccatcgtg aatcagcact gtgttgcgct ttaatgcttc caggtcgcca
      661 agaatagttt tgctatgttc gatgaagtaa cttcccgctg gcgataattc aatatagcgt
      721 cccttacgaa tgaacagttc aacgcccagg ctctcttccg ctttcttgat ggtgtaactg
      781 atggcggaag ggactttatt taactgctcg gctgcggtgg taatgctctg gtgtctggcg
      841 accagatgaa taacacgaag cgtttcgtct gagatgaaca taaggctccc tggtcagtgg
      901 taaagtggct gaatctctcc agattcagcc gtaatcattg cctgagtata cccaggaatt
      961 agcggtgttg acttgcccga ctgagaatcg catacgcgat acatgccacc agaatcgagt
     1021 ctaccgaagg gatcgtcgtc agggtgaata agccttttac ggtcataaag ccaaccgctg
     1081 cgcctatgat ccatgctgcg aatgtcagca ctttaatctg tgactcctgg gctaacactg
     1141 actcttgata gccattacgg cgataaagga aaaagtcagc gatataaatt ccggcaacgg
     1201 gtggcgtggc gatacccagg aacagcagaa acggaataaa ccacgccata atatccatac
     1261 tgcctacgat ggcggaaagg atacccagcg ccacggtaat ctgccatttg ggaaagcggg
     1321 taagcagtgt ggaaaccacc agcgtgccct ggaacatatt gcccgcgtta ccggtgacgc
     1381 aggcgaaaat caacaacagc gccgcaggca gaaccgcacc gaataccgcc atcgcaccca
     1441 gtaaagatcc ctgaccacta atggcgctcg gcgttgcacc cgcccagtag agcagagggt
     1501 aggcaaccag aaacgtcacg cctgcggcaa tcagcgcatg tttgcggtta tgcacaaaac
     1561 taccgaaatc aggcagtgtg gcaaccagca caataatggt gccaacaacg gtggaaacgg
     1621 cgacacctgt attcatctcc acaatcgccg ctggtactgc cacttcggta tgggttgcca
     1681 catagaggat gtaacacagc accagcgcga taaccggcac ggcaacctgg gcaatttttc
     1741 ccagaacggc aaagccaaat gccgtagagg cgacaaaaat gacacagcca gccgccacta
     1801 acagtgccag tggaacttcc agtccatgtt gagccagtaa gtcatgtacc gaatgaccaa
     1861 acatattggc ggtaacggca atccagccga acagacttat cgccatcaaa atgttaatgg
     1921 cgatggcccc tttttcgcca aacgaatatt tcacaatgcc atacgttggc aggcgggctt
     1981 tttctcccac gctaatggtg atggctgaaa gtaaggtcag aatgacgccg cctaacaata
     2041 cgacgctaat taaactgctg ctggaaagtt gattgccgag gcttgaggat gacaacagta
     2101 caggtgtgac ggcaataccc agcaaaatca tggcaatacg caatcctgat gccgtattca
     2161 gattctcttc ttttttacgc atagtatgtt ccctttataa atgcgcgtta cacagtatga
     2221 aaatgcccgg tgtatttgcg ccagctaccc agttctgtaa tttcccgttg gccttcggtc
     2281 agccagtttt ctgccagtac gcccagcact tcgctaccat cggcaagttt gattttgcca
     2341 attgccagcc cagcgggttc gctcattaac agagcggcaa aactggccag cggtaattgc
     2401 caaatttcta cgtcgacgtg tgttcctcct tcattcacgc gaatcattcc cggatggcga
     2461 tcattaatac tccacaaccg gtaatgggcg tcagttcggt cttcacggac aaaaataccg
     2521 cccgcttttt gcatatttgg gttgagttcc aggccgcgca tcagcgttcc gttaacggct
     2581 aaaagcgttg tagtcattgt gttctccaga gaattaaagg gttatccatg taccgctctg
     2641 atgcgccagg gctgctttta tggtttgcgg tgaagtaatc aggccgctgg cctgtttgcc
     2701 ttgttgctgg ctggcattga tgaaatcaac aatggcttcc actttgggtt gcatactgcc
     2761 gacaccaaac tgaccttccc gcaacagcgt ttgcgcttcg gcaacgctga tagcgtcgag
     2821 ccactgttgt tgtggtgtac caaagttaat cgctactttt tctacaccgg tggggatcac
     2881 cagcagatct gcgcctaact gactggcgag cagcgcggag gccagatctt tatcgatcac
     2941 ggcttctaca cctttcagtt gttgcccgtc tcgcaccacc ggaataccgc cgccgccgca
     3001 ggcaataacc agatatccct ggcgcaccag gtgagcgatg gtgtcgtgct caataatttc
     3061 cagtggtgca ggagagggaa ctgtacgccg ccaaccacgc ccggcgtcct ccatcagcgt
     3121 ccagccgagt tgttgttggc gttgttgggc cgttgcttca tcgagaaacg cgccaattgg
     3181 tttactgggg ctggcgaaag catcgtcatg tgggctgact cgcgtttgtg tcaccagggc
     3241 aattaccggt ttgtttatgc cacggcgagc caattcgtta tgcagcgctt tctggaacat
     3301 gtagccaatt gcaccttgtg tatcacccac ggcgtaatca agtggaaccg gagaaacttc
     3361 gttactggcg agttcagagc ggcgcagaat aaagcccacc tgcgggccat taccgtgggt
     3421 tagtaccagg tcccatccgg cttcaaccat atcaacgata tgttgcacgc tttccatcac
     3481 tgcaacatat tgatcgggaa tactattgcg ttgctcgtcc tgaatgagcg cattgccgcc
     3541 aaccgcgacg acagccagtg gcttaaccat gatggttctc ctttgcgacg atctccgcca
     3601 gtgcacgtac agcggcgaca aaacaggcca tcggcgcagt ggtgatcccg gcaccaattt
     3661 gcccgacccc cgcttgtttg tgggcgatac ccgtattgat gaccggtaaa atgccgcgat
     3721 cggtcacttt acgcgcatca attcctgctg cggtgggggc aaaattgagg gctggaagtg
     3781 taaatgcggg gttaccgccg agggtgatgg cttgcatccg acggctgtta ttggtggcat
     3841 cggcaggggt gcctcccaca aatttgacga tcgccggaga cgacgccatc gcgaagccac
     3901 ccatacctgc ggtttcagta attgcgctat cacccaggtc agcagcagcg tcgtcaacgc
     3961 caaatccggg gaagaacaga ccttcaacgg cattggcagg agcctggaac cagcgatcgc
     4021 tagtaccgga aagccgaata ccaaaattca ccccgttacg cgccatcacc gtaaccattg
     4081 aactgaacgg tacattggcg gcggcatcca tcattgcttt acaggcggcc atcgacagat
     4141 tgaggaagaa gtgatcgtta ccggtgataa atgccacgac gcgctggata tgttcctgtg
     4201 gaagagagca ggccagcaat gccgggagca ggcgcttaat cagcaaaccg gtagcggcag
     4261 cattgcggtt atgaacttca tcccccatat gcagcgcctg cgccattaat ggcttgagtt
     4321 ccagctcgcc gtgttgtgtg attgctgctt tcattgccgg cgccagttca tcgcgcatcc
     4381 aggccagacg attgagaact tcatcgttat tggcgccaaa acggagaacc ttacccagac
     4441 cttcattgaa attgctaaaa gtacggtggc cgttggtttt gttttctacc acccataacg
     4501 gcatcgacgg gctgatgatg cctgccattg ggccaaccgc gtgataatga tgacaagggg
     4561 caaggtcgat ttcgcctgcg tttatttggt tttcagcgtc ttgctggctg gtggcccaac
     4621 cttcgtacag catggcacca ataatcgccc cttttaccgg accacacatc tcttcccagg
     4681 caatcggcgg gccagagtgc aaaatacgtt tttgttcagc cattgctgga atggcctctt
     4741 tagcgagcat gacatccacc agcatgggct gtgcttcgag ataacgtgtc atggcgattt
     4801 ggttggcatt ttctaccagg ggatgacgta gcaacgatgc cagatccaat ccagcgtcaa
     4861 tatcgccttg tgctggtggc tgccagttga gtgcaattgc gcttcccccg gcatgttgaa
     4921 tattgtcagc aaaactgtgt agtcccgcgt tcacaacctt caaaggctga ttaaataagg
     4981 tattcatgcg tttagcccct ttttctgcaa ctgagtctgt gcaacggtgc tggcccatag
     5041 cgccgcctgt gcattgctgc tggcgatgat gacccctgca ttttgtagtg cgcttatctg
     5101 ctgactgcgt atttgcggat cagcttccgt tccgcaaacg tgcgcaatca gcaacggtgc
     5161 gttattcatg tcgatgtgtg aaagttggtc cagtaattcg ctggcaggcg ccgtggaagc
     5221 gccataacca agtaccaggt cgaataacac gacggcagtg tgtgagtcgt tgagttcgtt
     5281 gagcagacgt tgattgcgca gagtcgggtc gatcattgga tgcggtttgc cacgggtgaa
     5341 atcatcgtcg cccatatcga tcaacgtgtg atcttcgctt tgccagatat ttgccagagc
     5401 acgattaccc gcaactggcg tattggaagc tgcgctgaat cccttttgtt ggcagataag
     5461 ttgcgcttcg tagcagaagg ttcctccggc gaaaacaccg cgtatcgcct ggcgatgggc
     5521 tggcaggcgc tgacaggcgt tttgtaacat tgtgagatcg gcgcaggaaa tctcggtctc
     5581 gacagccgga agtggttgat cattcagtaa cgccacggcg atatttgcgg cgctggcaag
     5641 tgtggtggcc gcggtgatat tgggacgtgc cagatcatgc ggattcgcac cgaggaaatt
     5701 caccaccacg ggttttccac aagcctcggc gcgctccaga atagtccggg caacgatcgg
     5761 tgaaggcggt tttgaaatca gcacaattac gcgagtttca tcgtcttgcg ccagcgcatc
     5821 taaagcgaag agcatcgata ttccaccaat ctcttcactg agatcatgtc cgcctgttcc
     5881 caacgcctga gaaatacccg ctccgagctg gtcgattcgg caagtcactt cctgtagccc
     5941 ggtgcctgac gcgccaataa caccaattgc tccgcgtttc accacattag cgaagcctaa
     6001 cggaatgccg ttaacaatgg cggttccaca gtcaggtccc attacgatgc gctgacgttc
     6061 tcgggcaagc gttttgatgg atttttcctg cccgatactg acgttatcgc tgaacatcat
     6121 cacgttcatt ccgaggttta acgctttaat tgcttcggcg gcggcgtagt ctcccggtac
     6181 agaaatgagt gccaggttgg cttcgggttc ggcttccagt gccatttcca ggctgacctt
     6241 ttccggtgat ttaatgccac tatccgtttc atcatcgggt ttgctggtca gacgttgctg
     6301 ggccagtacc agtgcttcat tgcaaatatc ttcttcaccc atgactgcaa tcactaaatc
     6361 attgggactg gcgttgattt cgttacccag acccgcatcg cgcaattgtt cgagattatt
     6421 gggcgttccc atgacgaccg aggcctgttg aataccgggc aacttgctga tttgcgcgga
     6481 aatctgcatc agagaaactg agtcctgata aagattactg aaaactttat atttaacttt
     6541 catgctgtta cgcctgtttc attccgcccg atagcaaaat tcgggcaaag catccccgtg
     6601 tagccccata aacgggctac gggagaaaag gttgaaatca attcatggtg ttcagggttc
     6661 gaaagccatg cagcattcct gcaaggcaat ctactcctga cgttgcgcca aattgcatga
     6721 cctgttctgc acaggatgcg attgtcattg ccgatgcgct cccaaccagt tgtgcgagta
     6781 actggcaaat cggttctgaa aaatgtccct gaagcgcacg ctccagataa tgacgactga
     6841 tatccgtggt gttgtgcgca tgttgatcaa ttgcctgctg caaccggtat tgatgatcgg
     6901 caagtggtgc agggagttgc cataaccaga gtgctgccag atagcccaac aaatagtcat
     6961 ctccatcggg cgttaagcct ttaccaaagc cgatcagttg tggcgcctgg cgttccagcg
     7021 catctggatg ttccataatc gctaatccct ggtgcgtgag actatccggc aggtgcaatg
     7081 tgctttgaac gttatgctcc agacaaaata aaagccgttg cgatgccatc gttgggtagt
     7141 gctggaaaac caaagggaat gcctcaagtg tcagcattct ccccacaaat ggcgactgcc
     7201 agagcgttgc gttatcgagc gccacacaaa atcgaggtgt ccgcagcaag cctggggtta
     7261 actgaatggg atctgaatgg gcaatctctc ttcgccagtc ccaacattca ggtagccaga
     7321 ctctggcagc atcagggaga ttcagatacg tttgcgcaca caacagagtg atgaatgttc
     7381 ccgcgtctga atacagattt accgcatgtt taaaagcgct atgtagacgt aaatcttgcg
     7441 tttcattctg cgggaaaaga tatcccaacg acgatgcgca aagcgtaacc tgtaccatcg
     7501 gcgttagcct tccagacctg caataatggc tttggaatca gtaacccaac cgaaaatggc
     7561 accttgtgct ttgatcatct caagggcgta tttctggaat tccgggaaat aggagccgac
     7621 acaatcttcc gggataatgc attcatagcc acgatcattt gcttcgcgca cggtggtggt
     7681 tacgcaaact tcggttgtaa caccacaaac tatcagcgtc ttgataccgt ggttttgcaa
     7741 aatcagatgt aagtcggttt gataaaaagc gcctttgcca ggcttatcaa tcaccggttc
     7801 gcctgccacc gggtacagtt caggaatgat gtcatgacct gcttcacctc tgaccagaat
     7861 gcgtcccatc ggtcctggtt caccaataaa ggtttttccg ccgcgcgtca gtttggctgg
     7921 cgggcagtcg cttaagtctg cacgatggcc ttcacgagta tgaataacca taattccttt
     7981 ttgacgtgcg gctgccagca cctctgtaca tggtgcgata gccgtgcgta ccagtgaaac
     8041 atcattgccc agtgcctcac cgaagccccc ggcttcgaca aagtcacgct gcatgtcgat
     8101 catgaccagt gcagttgtgc gcggatcaaa aggtaattca aagggttgag cctgaaaaat
     8161 ggattgcgtc atggtaaatc tcctgtctga tgtttaaacg atccagttgt tctgagaacg
     8221 agtattcagg agaaaagcgg cacaaaacag gaaaaaagtt tgccgcacag caacgaaatt
     8281 tttgcattca gcaggtggtg tgatgacgaa tgtgaaggga gtcaaagagt ggcatacagc
     8341 aacaaatttt ttgatgctgt atggttttga gattaaaaaa atcgttgaaa ttgtgtgagc
     8401 ttaatcatca gatttagcga ctatggcgtt actttttaac accatactgt gtaaaccatt
     8461 ccaacattcg ctgccagccg tctttcgcag actcggcatg atagctcgga cgataatcgg
     8521 cgttgaacgc atgccctgca tccgggtaca cgataatctc tgctttcgcg tttgctgccc
     8581 gcagcgcctg gcgcatcgtt tccacgctct cttgcggaat gctgttatcc tggccgccat
     8641 ataagccgag aaccggagcg ttaagatcgg ttgcgatatc aacaggttgt ttcggtgaat
     8701 tcagcgactt atcgcccgtc agtttgccgt accacgccac tgcggctttt agctgtggat
     8761 tatgcgcggc atacagccag gtgatacgtc caccccagca gaatccggtg atcattaaac
     8821 gatgaacatc gccgccgttg cgggaagccc aactggcgac atgatcgaga tcggccagca
     8881 cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata tcggcaaaat
     8941 cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac ccctccagcg
     9001 ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc tgcactacaa
     9061 tgaccactgg cagtgggcca tcgctttgct ttggtctggc atggtaagca ggcatgttat
     9121 ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccgga gtctgaacga
     9181 tggtcgaagc gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt gttgccatgg
     9241 tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc atgcaacaaa
     9301 tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga gtgatttatg
     9361 tgatttgcat cacttttggc gggtaaattt atgcaacgca ttttcgtcat ggtgatgagt
     9421 atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct gactaaaccg
     9481 attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc tcactaaaaa
     9541 cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg tggaaaagat
     9601 cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca ccacctggcg
     9661 tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg gcccgtctac
     9721 ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc gtatcggtac
     9781 aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca cggcgtctgt
     9841 tcgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg tcgctgattt
     9901 cgaatgtacg actgcgctgg ttgaagctgc gaaatccatt ggcgcgacga ctcacgttgg
     9961 cgtgacagct tcttctgata ccttctaccc aggtcaggaa cgttacgata cttactctgg
    10021 tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg gcgtaatgaa
    10081 ctatgaaatg gaatctgcaa ccctgctgac catgtgtgca agtcagggcc tgcgtgccgg
    10141 tatggtagcg ggggttatcg ttaaccgcac ccagcaagag atcccgaatg ctgagacgat
    10201 gaaacaaacc gaaagccatg cggtgaaaat cgtggtggaa gcggcgcgtc gtctgctgta
    10261 attctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg cgtagcgcct
    10321 cgcaggaaac ttctttcaaa ctggacgttt gtacagcaca attctatttt gtgcgggtaa
    10381 gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg tttacgcagt tattgcattg
    10441 gtgggtgtgg caattggctg gctgtttgcc agttatcaac atgcgcagca aaaagccgag
    10501 caattagctg aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca acaaattacc
    10561 caaagcgagc actggcgcgc agagtgtgaa ttgctcaata acgaagtgcg cagcctgcaa
    10621 agtattaaca cctcgctgga ggccgatctg cgtgaagtga ccacgcggat ggaagccgca
    10681 cagcaacacg ctgacgataa aattcgccag atgatcaaca gcgaacagcg cctcagtgag
    10741 caatttgaaa accttgccaa ccgtattttt gaacacagta atcgtcgggt tgatgagcaa
    10801 aatcgccaga gtctgaacag tcttttgtcg ccgttgcgtg aacaactgga tggtttccgc
    10861 cgtcaggtcc aggacagctt tggtaaagaa gcccaagaac gtcataccct gactcacgaa
    10921 atccgcaacc tccagcaact caacgcccag atggcccagg aagcgatcaa cctgacgcgt
    10981 gcgctgaaag gcgacaataa aatccagggc aactggggcg aggtagtatt gacgcgggtg
    11041 ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag catcgaaaat
    11101 gacgcccgct cgcggatgca gccggatgtc atcgtgcggt tgccgcaggg aaaagatgtg
    11161 gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct atttcaacgc cgaagatgac
    11221 tactcccgcg aaagcgcgct acaggaacat atcgcgtcgg tacgtaacca tatccgtttg
    11281 ctgggacgca aagattatca acagctgccg gggctgcgaa ctctggatta cgtgctgatg
    11341 tttattcccg ttgaacccgc ttttttactg gcgcttgacc gtcagccgga gctgatcacc
    11401 gaagcattga aaaacaatat catgctggtt agcccgacta cgctgctggt ggcgctgcgc
    11461 actatcgcca acctgtggcg ttatgagcat caaagccgca acgcccagca aatcgccgat
    11521 cgtgccagca agctgtacga caagatgcgt ttgttcatcg atgacatgtc cgcgattggt
    11581 caaagtctcg acaaagcgca ggataactat cgccaggcaa tgaaaaaact ctcttcaggt
    11641 cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt taggggtaga aattaaacgc
    11701 gagattaatc cggatttggc tgaacaggcg gtgagccagg atgaagagta tcgacttcgg
    11761 tcggttccgg agcagccgaa tgatgaagct tatcaacgcg atgatgaata taatcagcag
    11821 tcgcgctagc ccattggggg tagttaagcc gggtagaaat ctagggcatc gacgcccaat
    11881 ctgttacact tctggaacaa ttttttgatg agcaggcact gagatggtgg ataagtcaca
    11941 agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg atatggtcgc
    12001 ccacgttttc cattccgtgg catcaaaata cgatgtcatg aatgatttga tgtcattcgg
    12061 tattcatcgt ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc gtgggcagac
    12121 cgtgctggat ctggctggtg gcaccggcga cctgacagcg aaattctctc gcctggtcgg
    12181 agaaactggc aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa tgggccgcga
    12241 gaagctgcgt aatatcggtg tgattggcaa cgttgagtat gttcaggcga acgctgaggc
    12301 gctgccgttc ccggataaca cctttgattg catcaccatt tcgtttggtc tgcgtaacgt
    12361 caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg gcggccgcct
    12421 gctggtactt gagttctcga agccaattat cgagccgctg agcaaagcct atgatgcata
    12481 ctccttccat gtgctgccgc gtatcggttc gctggtcgca aatgatgccg atagctaccg
    12541 ttatctggcg gaatccatcc gtatgcatcc tgatcaggat actctgaaag caatgatgga
    12601 agacgcaggc tttgaaagcg ttgactatta caacctgacg gcaggtgttg tggcgctgca
    12661 tcgtggttat aagttctgac aggagaccgg aaatgccttt taaaccttta gtgacggcag
    12721 gaattgaaag tctgctcaac accttcctgt atcgctcacc cgcgctgaaa acggcccgct
    12781 cgcgtctgct gggcaaagtg ctgcgcgtgg aggtaaaagg cttttcgaca tcattgattc
    12841 tggtgttcag cgaacgccag gttgatgtgc tgggtgaatg ggcgggcgat gctgactgta
    12901 ctgttatcgc ctacgccagc gtgttgccga aacttcgcga tcgccagcag cttgccgcgt
    12961 taattcgcag cggtgagctg gaagtacagg gcgatattca ggtggtgcag aacttcgttg
    13021 cactggcaga tctggccgag tgtgacccag cagaattgct ggcgccttat actggtgata
    13081 tcgccgctga aggtatcagc aaagccttgc gcggaggcgc gaagttcttg catcacggca
    13141 ttaaacgcca gcaacgttac gtggcggaag ccattaccga agagtggcgt atggcgccag
    13201 gtccacttga agtagcctgg tttgcggaag aaacggcagc cgttgaacgt gccgtggatt
    13261 ccctgaccaa acggctggaa aaactggagg ctaaatgacg ccaggtgaag tacggcgcct
    13321 atatttcatc attcgcactt ttttaagcta cggacttgat gaacttatcc ccaacatgcg
    13381 tatcaccctg ccgctacggc tatggcgata cacgctcttc tggatgccga accggcataa
    13441 agacaaacca ttaggtgagc ggctgcgact ggcgctgcaa gaactcgggc cggtgtggat
    13501 taagttcggg cagatgttat ctacccgccg cgacctgttc ccgccacata ttgccgatca
    13561 actggcgtta ttgcaggaca aagttgcgcc atttgatggc cgactggcga agcaacagat
    13621 tgaagcagca atgggtggcc tgccggtgga agcgtggttt gatgattttg atattaagcc
    13681 gttagcctct gcctctattg cccaggttca tactgcacgg ctgaaatcga atggcaaaga
    13741 ggtggtaatt aaagtcatcc gcccggatat tttgccggtc attaaagcag acctgaaact
    13801 tatctaccgt ctggctcgtt gggtaccgcg tttgctgccg gatggtcgtc gtctacgccc
    13861 aaccgaagtg gtgcgcgagt acgaaaaaac gttgatcgac gaactgaatt tgctgcggga
    13921 atctgccaac gccattcagc ttcggcgcaa ttttgaagac agcccgatgc tctacatccc
    13981 ggaagtttac cctgactatt gcagtgaagg gatgatggtg atggagcgca tttacggcat
    14041 tccggtttct gatgttgcga cgctggagaa aaacggcacc aacatgaaat tgctggcgga
    14101 acgcggcgtg caggtgttct tcactcaggt ctttcgcgac agctttttcc atgctgatat
    14161 gcaccctggc aacatcttcg taagctatga acacccggaa aacccgaaat atatcggcat
    14221 tgattgcggg attgttggct cgctaaacaa agaagataaa cgctatctgg cggaaaactt
    14281 tatcgccttc tttaatcgcg actatcgcaa agtggcagag ctacacgtcg attctggttg
    14341 ggtgccacca gataccaacg ttgaagagtt cgaatttgcc attcgtacgg tctgtgaacc
    14401 tatctttgag aaaccgctgg ccgaaatttc gtttggacat gtactgttaa atctgtttaa
    14461 tacggcgcgt cgcttcaata tggaagtgca gccgcaactg gtgttactcc agaaaaccct
    14521 gctctacgtc gaaggggtag gacgccagct ttatccgcaa ctcgatttat ggaaaacggc
    14581 gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt attcctgcgc tggtgagagc
    14641 atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg aactggttta
    14701 cgacagtttg cgccagggca agtatttaca gcatagtgtt gataagattg cccgcgagct
    14761 tcagtcaaat catgtacgtc agggacaatc gcgttatttt ctcggaattg gcgctacgtt
    14821 agtattaagt ggcacattct tgttggtcag ccgacctgaa tgggggctga tgcccggctg
    14881 gttaatggca ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac gctgattttt
    14941 tcatcgctca aggcgggccg tgtaacgtat aatgcggctt tgtttaatca tcatctacca
    15001 cagaggaaca tgtatgggtg gtatcagtat ttggcagtta ttgattattg ccgtcatcgt
    15061 tgtactgctt tttggcacca aaaagctcgg ctccatcggt tccgatcttg gtgcgtcgat
    15121 caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa ccagccagga
    15181 tgctgatttt actgcgaaaa ctatcgccga taagcaggcg gatacgaatc aggaacaggc
    15241 taaaacagaa gacgcgaagc gccacgataa agagcaggtg taatctgtgt ttgatatcgg
    15301 ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc gttctggggc cgcaacgact
    15361 gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac tggcgacaac
    15421 ggtgcagaac gaactgaccc aggagttaaa actccaggag tttcaggaca gtctgaaaaa
    15481 ggttgaaaag gcgagcctca ctaacctgac gcccgaactg aaagcgtcga tggatgaatt
    15541 acgccaggct gcggagtcga tgaagcgttc ctacgttgca aacgatcctg aaaaggcgag
    15601 cgatgaagcg cacaccatcc ataacccggt ggtgaaagac aatgaaactg cgcatgaagg
    15661 cgtaacgcct gctgctgcac aaacgcaggc cagttcgccg gaacagaagc cagaaaccac
    15721 gccagagccg gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg caccttcccc
    15781 ttcgtcgagt gataaaccgt aaacatgtct gtagaagata ctcaaccgct tatcacgcat
    15841 ctgattgagc tgcgtaagcg tctgctgaac tgcattatct cggtgatcgt gatattcctg
    15901 tgtctggtct atttcgccaa tgacatctat cacctggtat ccgcgccact gatcaagcag
    15961 ttgccgcaag gttcaacgat gatcgccacc gacgtggcct cgccgttctt tacgccgatc
    16021 aagctgacct ttatggtgtc gctgattctg tcagcgccgg tgattctcta tcaggtgtgg
    16081 gcgtttatcg ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc gctgctggtt
    16141 tccagctctc tgctgtttta tatcggcatg gcgttcgcct actttgtggt ctttccgctg
    16201 gcatttggct tccttgccaa taccgcgccg gaaggggtac aggtatccac cgacatcgcg
    16261 agctatttaa gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt tgaagtgccg
    16321 gtggcaattg tgctgctgtg ctggatgggg attacctcgc cagaagactt acgcaaaaaa
    16381 cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc gccagatgtc
    16441 ttctcgcaaa cgctgttggc gatccctatg tactgcctgt ttgaaatcgg tgtcttcttc
    16501 tcacgctttt acgttggtaa agggcgaaac cgggaagagg aaaacgacgc tgaagcagta
    16561 agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg cggttgtcat atggagtaca
    16621 ggatgtttga tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac cgtgatgatg
    16681 ttgtagcgcg cgcttttgac gcgggagtta atgggctact catcaccggt accaatctgc
    16741 gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg tcaacggcgg
    16801 gcgtacatcc tcacgacagc agccagtggc aagctgtgac tgaagaagcg attattgagc
    16861 tggccgcgca gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt aaccgcaact
    16921 tttcgacgcc ggaagagcag gaacgcgctt ttgttgccca gctacgcatt gccgcagaat
    16981 taaacatgcc ggtatttatg cactgtcgcg atgcccacga gcggtttatg acattgctgg
    17041 agccgtggct ggataaactg cctggtgcgg ttcttcattg ctttaccggc acacgcgaag
    17101 agatgcaggc gtgcgtggcg tgtggaattt atatcggcat taccggttgg gtttgcgatg
    17161 aacgacgcgg gctggagctg cgggaattgt tgccgttgat tccggcggag aaattgctga
    17221 tcgaaactga tgcgccgtat ctgctccctc gcgatctcac gccaaagcca tcatcccggc
    17281 gcaacgagcc agcccatctg ccccatattt tgcaacgtat tgcgcactgg cgtggagaag
    17341 atgccgcatg gctggctgcc accacggatg ccaatgtcaa aacactgttt gggattgcgt
    17401 tttagagttt gcggaactcg gtgttcttca cactgtgctt aatctcttta ttaataagat
    17461 tcagcaatag catggagcga gcctcgccat cgggctcggt gaaaatggcc tgaaaacctt
    17521 cgaatgcgcc ttcagtaata atcactttat caccaggata cggcgtcgat ggatcgacaa
    17581 tatctttcgg tttatacact gataactgat gaatcaccgc cgatgggact atcgctggcg
    17641 acgcgccaaa gcgcacgaag tggctgacac cacgagtcgc gttgatagtc gtggtatgaa
    17701 tcacttccgg atcgaactcc acaaacaagt agttggggaa caatggctca ctgactgcag
    17761 tacgtttgcc acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa ttcacagcct
    17821 gtctttcgag gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac agtaaatacc
    17881 aggattgcat aatgactctt atccgtttaa tcggggcgca aggatagcaa aagctttacg
    17941 ctaagttaat tatattcccc ggtttgcgtt ataccgtcag agttcacgct aatttaacaa
    18001 atttacagca tcgcaaagat gaacgccgta taatgggcgc agattaagag gctacaatgg
    18061 acgccatgaa atataacgat ttacgcgact tcttgacgct gcttgaacag cagggtgagc
    18121 taaaacgtat cacgctcccg gtggatccgc acctggaaat cactgaaatt gctgaccgca
    18181 ctttgcgtgc cggtgggcct gcgctgttgt tcgaaaaccc taaaggctac tcaatgccgg
    18241 tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat gggcatgggg caggaagatg
    18301 tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct gaaagagccg gagccgccaa
    18361 aaggtttccg cgacctgttt gataaactgc cgcagtttaa gcaagtattg aacatgccga
    18421 caaagcgact gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat gacgtcgatc
    18481 tcaatcgtat tcccattatg acctgctggc cggaagatgc cgcaccgctg attacctggg
    18541 ggctcaccgt aacgcgcggc ccgcataaag agcggcagaa tctgggcatt tatcgccagc
    18601 agctaattgg taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc ggcgcgttgg
    18661 attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct gtggcgctgg
    18721 gtgccgatcc tgccacgatt ctcggtgcag tcacccccgt tccggatacg ctttcagagt
    18781 atgcgtttgc cggattgctg cgcggcacca agaccgaagt ggtgaagtgt atctccaatg
    18841 accttgaagt gcccgccagt gcggagattg tgctggaagg gtatatcgaa caaggcgaaa
    18901 ctgcgccgga agggccgtat ggcgaccaca ccggttacta taacgaagtc gatagtttcc
    18961 cggtatttac cgtgacgcat attacccagc gtgaagatgc gatttatcat tccacctata
    19021 ccgggcgtcc gccagatgaa cctgcggtac tgggtgtagc actgaacgaa gtgttcgtgc
    19081 cgattctgca aaaacagttc ccggaaattg tcgattttta cctgccgccg gaaggctgtt
    19141 cttatcgtct ggcggtagtg acgatcaaaa aacagtacgc cggacacgcg aagcgcgtca
    19201 tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac taaatttgtg atcgtttgcg
    19261 atgatgacgt caacgcacgc gactggaacg atgtgatttg ggcgattacc acccgtatgg
    19321 acccggcgcg ggatactgtt ctggtagaaa atacgcctat tgattatctg gattttgcct
    19381 cgcctgtctc cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa tggccggggg
    19441 aaacccagcg tgaatgggga cgtcccatca aaaaagatcc agatgttgtc gcgcatattg
    19501 acgccatttg ggatgaactg gctattttta acaacggtaa aagcgcctga tgcgcgtttg
    19561 ttttgcccta tttatcgatc cgacagagaa agcgcatgac aaccttaagc tgtaaagtga
    19621 cctcggtaga agctatcacg gataccgtat atcgtgtccg catcgtgcca gacgcggcct
    19681 tttcttttcg tgctggtcag tatttgatgg tagtgatgga tgagcgcgac aaacgtccgt
    19741 tctcaatggc ttcgacgcca gatgaaaaag ggtttatcga gctgcatatt ggcgcttctg
    19801 aaatcaacct ttacgcgaaa gcagtcatgg accgcatcct caaagatcat caaatcgtgg
    19861 tcgatattcc ccacggagaa gcgtggctgc gcgatgatga agagcgtccg atgattttga
    19921 ttgcgggcgg caccgggttc tcttatgccc gctcgatttt gctgacagcg ttggcgcgta
    19981 acccaaaccg tgatatcacc atttactggg gcgggcgtga agagcagcat ctgtatgatc
    20041 tctgcgagct tgaggcgctt tcgttgaagc atcctggtct acaagtggtg ccggtggttg
    20101 aacaaccgga agcgggctgg cgtgggcgta ctggcaccgt gttaacggcg gtattgcagg
    20161 atcatggtac gctggcagag catgatatct atattgccgg acgttttgag atggcgaaaa
    20221 ttgcccgtga cctgttttgc agcgagcgta atgcgcggga agatcgcctg tttggcgatg
    20281 cgtttgcatt tatctgagat ataaaaaaac ccgcccctga caggcgggaa gaacggcaac
    20341 taaaacttta ttcagtggca tttagatcta tgtcgcatcc ggcaattggt gcacgatgcc
    20401 tgatgcgacg ctggcgcgtc ttatcaggcc tacattggtg ccggatcggt agaccggata
    20461 aggcgttcac gccgtatccg gcatacagtg cccgatgcga cgctgccgcg tcttatcggg
    20521 cctacaaaag ttctgaaccg tagaccggat aaggcgttca cgccgcatcc ggcaagtggt
    20581 taaacccgct caaacaccgt cgcaataccc tgacccagac cgatacacat cgtcgccaga
    20641 ccaaactgaa cgtctttgcg ctccatcaga ttcagcagcg tggtgctgat acgcgcaccg
    20701 gaacaaccca gcggatgacc cagcgcgatc gcgccaccgt tgaggttgat cttctcgtca
    20761 atctgctcca ttaacccgag atctttaata cacggcagga tctgcgcggc aaaggcttcg
    20821 ttcatctcga atacgccgat atcgctggca gaaagccccg cttttttcag cgccagcttc
    20881 gaggccggaa ccggaccata acccataatc gatgggtcac aacccacaac cgccatcgaa
    20941 cgcacgcagg cgcgcggctt aagacctaag tcacgggcgc ggctttcact catcaccagc
    21001 atggcagctg cgccatcgga aagcgcagaa gatgtacccg ccgttaccgt accgctgacg
    21061 ggatcaaacg ccggacgcag tgtggcgagg gcttccacgg tggtttccgg gcgaatcact
    21121 tcgtcgtaat taaactgctt caatacgccg tcggcatcgt gaccaccggt cgggatgatt
    21181 tcatttttaa atgcgcctga ctgcgtggcg gcccacgcgc gggcgtgtga ccgcgcggca
    21241 aaggcatcct gcatttcacg gctgataccg tgcatacgcg ccagcatttc tgccgttaag
    21301 cccatcatgc ccgccgcttt ggcgacattg cggctcaggc cggggtgaaa atcgacaccg
    21361 tggctcattg gcacatggcc catatgctcc acgccgccaa ccagacatgc ctgcgcatcg
    21421 ccagtcataa tcatccgtgc tgcgtcatgc agtgcctgca tggatgaacc acacaagcga
    21481 ttgacggtca ccgccgggac agagtgcggc acttctgcca acagcgccgc gttacgggcg
    21541 atgttaaaac cctgctccag tgtttgctgc acacaacccc agtaaatatc gtcgagggcc
    21601 gctgcttcca gcgccgggtt acgcgccagc aggctacgca ttaaatgagc ggagagatct
    21661 tccgcacgca cgtgacgaaa agcaccgccc ttcgaacggc ccatcggggt gcgaattgca
    21721 tcgacaatga caacctgttc cattgtgact ccttaagccg ttttcaggtc gccaaccgga
    21781 cgggctggct caaccggggg atagtacggt tcgttatgac gtgctttatt acgcagacct
    21841 tccggtactt catacagcgg gccgaggtgc tgatattgct gtgccatatc gaggtatttt
    21901 gcgctgccga gggtgtccag ccagcggaac gcgccgccgt ggaacggagg gaagcccagg
    21961 ccgtagacca gcgccatatc cgcttctgcc ggagtggcga taatgccctc ttccagacag
    22021 cggaccactt cgttgaccat cgggatcatc atgcgggcga taatctcttc ttcgctgaaa
    22081 tcgcgctttg cgtggctgac ctctgccagc aggtcgtcaa cgacggcatc ttcttctttc
    22141 ttcggcttac ctttgctgtc ttctttataa cgccagaaac cgaggccgtt cttctgacca
    22201 aagcggttgg cattaaacag cgcgtcgatg gcatcgcgat aatctttctg catccgctgc
    22261 gggaagcctg ctgccatgac agcctgggcg tgatgcgcgg tgtcaatgcc cacaacatcc
    22321 agcagatatg ccggacccat cggccagcca aactgttttt ccatcacttt gtcgatcttg
    22381 cggaaatccg cgccgtcgcg cagcaactgg ctgaaaccgg cgaaataggg gaacagtaca
    22441 cggttgacga agaagccggg gcagtcgtta accacaatcg gcgttttgcc catcttgctc
    22501 gcccaggcga cgactttcgc gatggtttcg tcggagcttt tctcgccgcg aataatttct
    22561 actaacggca ttcggtggac cgggttaaag aagtgcatcc cgcagaagtt ttccggacgt
    22621 tccagcgcgt tggccagttc gctgatagga atggttgaag tgttagacgc cagtacggta
    22681 tccgggcgta ctttctgctc ggtttctgcc agcactgctt ttttcacttt cgggttttca
    22741 acaaccgctt ctaccacaac atccacgcgg tcaaacccgg cgtagttgag cgttgggtga
    22801 attgtggaga tcacgccagc cagtttcaga ccatcgatct tgccgcgctc aagctgcttg
    22861 ttcagcagtt tcgcggcttc ggtcatgccg agggttaacg acttgtcgtt gatatctttc
    22921 atgacaaccg gcacgccttt ccacgcagac tggtaggcga tgccgccgcc cataatgcct
    22981 gcgcccagca cagcggcctg tttcggggtt tcaacatctt tggtgagttt cttcgcttta
    23041 ccttttacat attgatcgtt aaggaaaatg ccgaccagtg cgcgggcttc gttggtatgc
    23101 gccagcggga caaaactttt gttttccagg tttaaggctt cttcacgacc aaaacgggcc
    23161 gcagcttcaa tggtttttac tgcggtgatg ggggccggat aatgtttccc cgccgtttgc
    23221 gcgaccatcc ctttagcgat ggtgaagctc atggcggctt caatcttgct cagttttagc
    23281 ggttccagct tcggctgacg ttttgctttc cagtcgaggt cgccgttaat ggcctggcgt
    23341 aaaatcgcta tcgccccttc aaccagtttt tctgctttga ctacgccatc caccagaccg
    23401 attttcagcg cctgatccgc gccgacatct ttaccggcgg caatgatttc cagcgcactg
    23461 tcagcgccca gcatacgtgg catacgtaca gaaccgccaa agcccggcat gatgcccaat
    23521 ttggtttccg gcagaccgat gcgcagatcc ggcgtcgcca gacgataatc ggtcgccagc
    23581 acgcattcgc agccaccgcc cagcgcatag ccgttgacgg cagcaatggt cggcaccggg
    23641 agatcttcca ggcgattaaa cacgctattg gcaaagtgca gccactgact taactgttct
    23701 tcaggaacga ggaacaggga caaaaattcg gtgatatcag caccgacgat aaaggctgct
    23761 ttgttcgaac gcagcagcag cccttttaga tctgattgct gttccagcac gccgatggcc
    23821 tcgccgaggc tggcgacggt ggcagtgtcg agtttattaa ctgaacctgg ggcatcaaat
    23881 accagttcgg caatgccatc ttccagccag tcaaggtaca gggtgtcgcc tttgtaaagc
    23941 atgtcagtct cctgaatccg caaggtgatc tggtcgtacc agatgcatcg aagtgtgtat
    24001 tttgtgttaa aaatatgcaa ataaaagatt aaagaaatgc cgatctgatc acgctcggca
    24061 gaaatcacgc tctggatgaa cgatgtgcta agatgcggag acttaaggtc aaaaaaacag
    24121 aagggtaaaa aatggaatca ctggcctcgc tctataaaaa tcatatagct accttacagg
    24181 aacggactcg cgatgcgctg gcgcgcttca agctggatgc gttacttatt cactccggcg
    24241 aactgttcaa cgtttttctc gacgatcatc cctatccgtt taaagtgaac ccgcaattca
    24301 aagcgtgggt gccagtaact caggtgccaa actgctggct gctggtggat ggcgtgaata
    24361 agccgaaact gtggttttat ctgccggttg attactggca caacgtcgaa ccgctgccga
    24421 actccttctg gactgaagat gtagaagtga tcgcgctgcc gaaagccgat ggcattggta
    24481 gtctgttgcc tgctgcgcgc ggcaatatcg gttatatcgg tccggtgcca gaacgtgcgc
    24541 tgcaactggg tattgaggcc agcaatatca acccgaaagg ggtgatcgac tacctgcatt
    24601 actatcgctc cttcaaaacc gagtacgaac tggcctgtat gcgtgaagcg cagaaaatgg
    24661 cggtcaacgg tcatcgcgcg gcagaagaag cgttccgttc tggcatgagc gagttcgata
    24721 tcaacattgc ctatctgacc gcgaccggtc atcgtgatac cgacgtacct tatagcaaca
    24781 ttgtggcgct taacgaacac gctgcggtgt tgcattacac caaactggac caccaggcac
    24841 cggaagagat gcgcagcttc ctgctggatg ccggggccga atataacggc tatgcggctg
    24901 acctgacccg tacctggtcg gcaaaaagcg acaacgacta cgcacagcta gtgaaagacg
    24961 taaatgatga acaactggcg ctgatcgcca ccatgaaagc tggcgtcagc tatgtggatt
    25021 accacatcca gttccatcag cgcatcgcca aattgctgcg taaacatcaa atcatcaccg
    25081 atatgagtga agaagcgatg gtcgaaaacg atctcaccgg accgtttatg ccgcacggta
    25141 tcggtcatcc gctgggcctg caggtgcatg acgtagctgg ctttatgcaa gatgatagcg
    25201 gtacgcacct cgcggcaccg gcaaaatatc cgtacctgcg ctgcacccgt attctccagc
    25261 cgggcatggt gttaaccatc gaaccgggta tctacttcat cgaatcgctt ctggcaccgt
    25321 ggcgtgaagg gcagttcagc aaacacttca actggcagaa aattgaagca ctgaaaccgt
    25381 tcggcggcat tcgtatcgaa gacaacgtgg tgattcacga aaacaacgtg gaaaacatga
    25441 cccgggatct gaaactggcg tgatggaaag ctggttaatt cctgcggcac cggtcacggt
    25501 cgttgaagag atcaaaaaga gccgcttcat tacgctgttg gcgcataccg atggcgttga
    25561 ggcggcaaaa gcgtttgttg aatcggtgcg ggcagaacac cccgatgccc gccaccattg
    25621 cgtggcgtgg gtggcgggtg cgccggatga ttctcaacag ctgggtttct ctgacgacgg
    25681 ggagccggcg ggaacggcag gtaaaccgat gctcgcccaa ctaatgggca gcggcgtcgg
    25741 ggaaattacc gctgtggtgg tgcgctacta cggcggcata ttgcttggca ccggtgggtt
    25801 agtgaaagcg tatggcggcg gcgtgaatca ggcgctgcgc cagctaacga cccaacgcaa
    25861 gacaccatta accgaatata ctttgcaatg tgaatatagt cagttaaccg gcattgaagc
    25921 gttgctgggg cagtgtgacg gcaaaattat caacagtgat tatcaggcat tcgttctgct
    25981 gcgggtggcg cttccggcgg cgaaagtggc tgaattttcc gcaaagctgg cggattttag
    26041 ccgtggttca ttgcaattgt tagcgattga agaataatcc ccacttcgtt ttgcagaact
    26101 aaggaagcgg cagagatgca ttttcgcgcc attacccgaa tcgttggact actggttatc
    26161 ttattttcag ggaccatgat tatccctggg ctggtagcac tcatctaccg ggatggagcg
    26221 ggccgcgctt ttacccagac cttttttgtc gccctcgcca ttggctctat gctttggtgg
    26281 ccgaaccgca aagagaaggg cgaactgaaa tcccgtgagg ggtttctgat agtggtgctg
    26341 ttctggaccg tgctgggtag cgtcggtgcg ctccctttta tcttctcgga aagcccgaac
    26401 ctcacgatta ccgatgcgtt ttttgaatct ttctctggcc tgaccaccac gggggccact
    26461 acgctggtgg ggctggattc gctccctcat gccatcctct tttatcgcca gatgctgcaa
    26521 tggtttggcg gtatggggat catcgtgctg gcggttgcga tactgcctat cctcggcgtg
    26581 ggtgggatgc agctctatcg cgcagaaatg cccggcccgc tgaaagataa caaaatgcgc
    26641 ccgcgaattg cggaaacggc gaaaaccctg tggttgattt atgtcttgct gaccgtcgcc
    26701 tgtgcgctgg cgttgtggtt tgctggaatg gatgcctttg atgccatcgg ccatagcttt
    26761 gcgactatcg ctattggcgg cttctcgaca catgatgcca gtatcggtta tttcgacagc
    26821 ccgactatta acactatcat tgctatcttc ctgctgatct ctggctgtaa ctacggtctg
    26881 cacttttcac tgttaagtgg gcgtagtctg aaggtttatt ggcgcgatcc ggaatttcgc
    26941 atgtttatcg gcgtacagtt ttcactggtg gttatttgta cactcgtact gtggtttcat
    27001 aatgtctaca gttcggcgct gatgacaatt aaccaggcgt ttttccaggt ggtgtcgatg
    27061 gcgacaaccg ccgggtttac aactgacagc attgcccgct ggccgctctt tttgccggta
    27121 ctgcttttat gttcagcttt tatcggcggt tgcgccgggt caacgggcgg tggcctgaaa
    27181 gtgatccgca tcctgttgct gtttaagcag gggaaccgtg agctgaaacg actggtgcat
    27241 ccgaacgccg tctatagcat taagctgggg aatcgcgcac tgccggaacg tatcctcgaa
    27301 gccgtttggg gatttttctc cgcctatgca ttggtgttta ttgtcagtat gctggcgatt
    27361 atcgccacgg gcgtggatga cttttctgcc tttgcctcgg ttgttgcaac attgaataac
    27421 ctggggccag ggcttggcgt ggttgctgat aactttacca gtatgaaccc ggtggctaaa
    27481 tggatcctga ttgccaacat gctgtttggt cgtctcgagg tctttacatt gctggtgctc
    27541 tttaccccga ctttctggcg tgaatgatgg agtaatacgt gaaaacatta attcttttct
    27601 caacaaggga cggacaaacg cgcgagatcg cctcctacct ggcttcggaa ctgaaagaac
    27661 tggggatcca ggcggatgtc gccaatgtgc accgcattga agaaccacaa tgggaaaact
    27721 atgaccgtgt ggttattggc gcttctattc gctatggtca ctaccattct gcgttccaag
    27781 aatttgtcaa aaagcatgcg acgcggttga attcgatgcc gagtgccttt tactccgtaa
    27841 atctggtggc gcgtaaaccg gagaagcgta ctccacagac caacagctac gcgcggaagt
    27901 ttctgatgaa ctcgcaatgg cgtcccgaac gctgtgccgt cattgccggg gcgctgcgtt
    27961 acccacgtta tcgctggtac gaccgtttta tgatcaagct gattatgaag atgtcaggcg
    28021 gtgaaacgga tacgcgcaaa gaagttgtct ataccgattg ggagcaggtg gcgaatttcg
    28081 cccgagaaat cgcccattta accgacaaac cgacgctgaa ataagcataa agaa
//
LOCUS       DADSZD010000046        27939 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.046, whole genome shotgun sequence.
ACCESSION   DADSZD010000046 DADSZD010000000
VERSION     DADSZD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.046"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="J1Z28_001776"
     CDS             complement(153..1328)
                     /gene="ubiF"
                     /locus_tag="J1Z28_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBA7716616.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            1474..2898
                     /gene="miaB"
                     /locus_tag="J1Z28_001777"
     CDS             1474..2898
                     /gene="miaB"
                     /locus_tag="J1Z28_001777"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBA7716617.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3012..4091
                     /locus_tag="J1Z28_001778"
     CDS             3012..4091
                     /locus_tag="J1Z28_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBA7716618.1"
                     /translation="MFSFRRHTDKRNGLNIDTREITLEPADNARLLSLCGPFDDNIKQ
                     LERRLGIEINRRDNHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAI
                     KEARVLEQSAESVPEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTG
                     KTYLAVAAAVDALERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFE
                     MLGFEKVEKLIERNVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNS
                     KAVITGDVTQIDLPRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYE
                     AWEEAEQKRKAALAAERKREEQEQK"
     gene            4088..4555
                     /gene="ybeY"
                     /locus_tag="J1Z28_001779"
     CDS             4088..4555
                     /gene="ybeY"
                     /locus_tag="J1Z28_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBA7716619.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            4645..5523
                     /gene="corC"
                     /locus_tag="J1Z28_001780"
     CDS             4645..5523
                     /gene="corC"
                     /locus_tag="J1Z28_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBA7716620.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5548..7089
                     /gene="lnt"
                     /locus_tag="J1Z28_001781"
     CDS             5548..7089
                     /gene="lnt"
                     /locus_tag="J1Z28_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBA7716621.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGSYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRKR"
     gene            complement(7221..9098)
                     /locus_tag="J1Z28_001782"
     CDS             complement(7221..9098)
                     /locus_tag="J1Z28_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBA7716622.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSDESWRLLSSVFLHSSFSHLLMNMFALLVVGTVAERILGKWRLLI
                     IWLFSGIFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGTRQTGIDNACHIGGLIAGGALGWLSARLVGQNRFVTEGGIIV
                     AVTLLLTGAIWLVQQQIDESVLQVRQSLREAFYPQEIEQERRQKKQQLVEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSLDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGMSGKPGAWV
                     MALSPDEKTLLVPGAGRGSIVRINTITHQKEDFPAGNARGVVSAMGFRPKNGEVIFAD
                     SQGISRIRAEDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNNLRFSADGRKIFVMAKNGLIQLDRESSLDPQAIIRHPQYGDVACIPE
                     P"
     gene            9552..10460
                     /gene="gltI"
                     /locus_tag="J1Z28_001783"
     CDS             9552..10460
                     /gene="gltI"
                     /locus_tag="J1Z28_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBA7716623.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10630..11370
                     /gene="gltJ"
                     /locus_tag="J1Z28_001784"
     CDS             10630..11370
                     /gene="gltJ"
                     /locus_tag="J1Z28_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBA7716624.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            11370..12044
                     /gene="gltK"
                     /locus_tag="J1Z28_001785"
     CDS             11370..12044
                     /gene="gltK"
                     /locus_tag="J1Z28_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBA7716625.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12044..12769
                     /gene="gltL"
                     /locus_tag="J1Z28_001786"
     CDS             12044..12769
                     /gene="gltL"
                     /locus_tag="J1Z28_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBA7716626.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12887..13822
                     /gene="rihA"
                     /locus_tag="J1Z28_001787"
     CDS             12887..13822
                     /gene="rihA"
                     /locus_tag="J1Z28_001787"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBA7716627.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLCESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(13867..14349)
                     /locus_tag="J1Z28_001788"
     CDS             complement(13867..14349)
                     /locus_tag="J1Z28_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBA7716628.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            14584..17166
                     /gene="leuS"
                     /locus_tag="J1Z28_001789"
     CDS             14584..17166
                     /gene="leuS"
                     /locus_tag="J1Z28_001789"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBA7716629.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            17181..17762
                     /gene="lptE"
                     /locus_tag="J1Z28_001790"
     CDS             17181..17762
                     /gene="lptE"
                     /locus_tag="J1Z28_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBA7716630.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            17762..18793
                     /gene="holA"
                     /locus_tag="J1Z28_001791"
     CDS             17762..18793
                     /gene="holA"
                     /locus_tag="J1Z28_001791"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBA7716631.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMSEALNRLSQSQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            18795..19436
                     /gene="nadD"
                     /locus_tag="J1Z28_001792"
     CDS             18795..19436
                     /gene="nadD"
                     /locus_tag="J1Z28_001792"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBA7716632.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSMQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPGVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            19460..20071
                     /locus_tag="J1Z28_001793"
     CDS             19460..20071
                     /locus_tag="J1Z28_001793"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBA7716633.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARRVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIWVENA"
     gene            20331..20648
                     /gene="rsfS"
                     /locus_tag="J1Z28_001794"
                     /gene_synonym="rsfA"
     CDS             20331..20648
                     /gene="rsfS"
                     /locus_tag="J1Z28_001794"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBA7716634.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            20652..21119
                     /gene="rlmH"
                     /locus_tag="J1Z28_001795"
     CDS             20652..21119
                     /gene="rlmH"
                     /locus_tag="J1Z28_001795"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010618862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBA7716635.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            21150..23051
                     /gene="mrdA"
                     /locus_tag="J1Z28_001796"
     CDS             21150..23051
                     /gene="mrdA"
                     /locus_tag="J1Z28_001796"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBA7716636.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            23054..24166
                     /gene="mrdB"
                     /locus_tag="J1Z28_001797"
                     /gene_synonym="rodA"
     CDS             23054..24166
                     /gene="mrdB"
                     /locus_tag="J1Z28_001797"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBA7716637.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            24177..25265
                     /gene="rlpA"
                     /locus_tag="J1Z28_001798"
     CDS             24177..25265
                     /gene="rlpA"
                     /locus_tag="J1Z28_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBA7716638.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            25405..26616
                     /gene="dacA"
                     /locus_tag="J1Z28_001799"
     CDS             25405..26616
                     /gene="dacA"
                     /locus_tag="J1Z28_001799"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBA7716639.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            26726..26989
                     /locus_tag="J1Z28_001800"
     CDS             26726..26989
                     /locus_tag="J1Z28_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBA7716640.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            27090..27731
                     /gene="lipB"
                     /locus_tag="J1Z28_001801"
     CDS             27090..27731
                     /gene="lipB"
                     /locus_tag="J1Z28_001801"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBA7716641.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
BASE COUNT         6795 a         7296 c         7198 g         6650 t
ORIGIN      
        1 attaaaaaag ctcgcttcgg cgagcttttt gcttttctgc gtatattcaa tgccgaatgc
       61 gatgttgact cgtcttatcc ttcaaagtcg gatgcgacgc tgccgcgtct tatccgacct
      121 acggttggca tgcatccagc aatgttgtga ggctacaacc ctaacgcata tttcagcgcc
      181 ttacgtttca acacgccagc acgctccgcc gccatcaaac caagattacg cacaaaacgc
      241 agcggtggca gattattgct gaatccggca taaaacagat ccataccact ttgcatcatg
      301 aagttatccg ccatacgccg catctggtaa cgcttgagca caggataact ggcccacgct
      361 tcgccgtagc tgcgggcgtt caccagaacg tcaatcaggg catcgacatc acgataacca
      421 agattcactc cctgccccgc cagcggatgg atggtatgcg cggcatcgcc caccagagca
      481 agccctggtt gcacatactg caacgcatgg cgacgtatca gcggaaacgc accagcggca
      541 agcggtgtta cgtaacccag acgcgacggg aaatgcttcg cgatttccgc ctgtagctgc
      601 gccatattca tattctgcaa ctggcgaatg cgcgccggag agtcatacca caccagcgat
      661 gcccagttat caaacaacgg cagaaacgca cgcggtccgt ccggggtaaa ttgctgccag
      721 gtgctgtcgc cgggatcgtt ctcacactgt acgctaatta acatacacga ctgcgcatac
      781 tgccaggcat gaacgccaat tcccgccatc tgtcgcacct gcgaatttgc gccgtcggca
      841 ccaatcacca gcttcgcgag gatcgtttca ccgcccttca gttccagctc ctgaagatca
      901 ttatggcgat gcagtgcaat cagcgagcct ggcacacgta acgttacttt cggatgcgct
      961 tccagcgcct gccacaacgc ctgttgcagg acagtgtttt ccaccatgta gccaagcaac
     1021 ggtagcttaa gttcagcggc gtcaaacacg acatgcgccg tttcccactc ccacgtttcc
     1081 agtctgcggt aaggatggca acgcatagcc tgtactgcat cccagacccc taacccttta
     1141 agcaatgata ccgaagccgc gctgatcgcc gagatccgca cgtccggttg gctgtcagcg
     1201 acaaacggcg caggttctgc gtgctcgatc accgttaccg caaatccgtg ctgtgccagc
     1261 cccagcgcca gtgcgccgcc gaccattcct ccgccgacaa tggcaatttc cgttggttga
     1321 tttgtcatgg tcaatcatcc tgttagcaat atgctttaag tttacaggat ttttaccccc
     1381 aagggctgat aacgctcata ctggtcacaa cggcagcaaa gcattacact atgcgccctg
     1441 cattcctggc tactatttcg caagagcaag tcgatgacca aaaaactcca tattaaaacc
     1501 tggggctgtc agatgaacga gtacgattca tcgaagatgg ccgatctgct ggatgccacc
     1561 cacggctatc aactgaccga cgtggcggaa gaagcggatg tgctgctgct gaacacctgc
     1621 tcaatccgcg agaaggctca ggaaaaagtc ttccatcagt tgggtcgctg gaaactgtta
     1681 aaagagaaga atccagacct gattatcggc gtcggcggct gcgtggcatc gcaagaaggc
     1741 gagcacattc gccagcgcgc ccactatgtc gatattattt ttgggccgca aacgctgcac
     1801 cgcctgccgg agatgatcaa ctccgtgcgc ggcgaccgca gcccggttgt agatatcagc
     1861 ttcccggaaa tcgagaagtt tgaccgtctg ccagaaccgc gcgccgaagg gccgaccgcg
     1921 tttgtctcca tcatggaagg ctgcaataaa tattgcacct actgcgtggt gccttacacc
     1981 cgtggtgaag aggtaagccg tccgtccgac gatattctgt ttgagatcgc ccagcttgcg
     2041 gctcagggcg tgcgtgaagt caacctgctc ggtcagaatg tgaatgcctg gcgtggtgag
     2101 aactacgacg gcaccaccgg aacgtttgcc gatctgctgc gtctggttgc tgcgatcgac
     2161 gggatcgatc gtattcgctt taccaccagc catccgatcg aattcaccga cgatatcatc
     2221 gaagtgtatc gcgacacgcc ggagctggtg agcttcctgc acctgccggt acagagcggt
     2281 tccgatcgca ttctgaacct gatgggccgt actcacacgg cgctggagta caaagcgatc
     2341 atccgtaaac tgcgtgcggc gcgtccggat attcagatca gttctgactt catcgttggc
     2401 ttccctggcg aaaccaccga cgacttcgag aaaacgatga agctgattgc cgacgtcaat
     2461 ttcgacatga gctacagctt tatcttctcc gcacgtccgg gtacgccagc cgccgatatg
     2521 gttgatgatg ttccggaaga agagaagaag cagcgtctgt atattctgca agagcgcatt
     2581 aatcagcagg cgatggcatg gagccgccgg atgctcggca ctacccaacg tattctggtg
     2641 gaagggactt cgcgtaagag catcatggag ctttccggtc gcactgaaaa taaccgcgtg
     2701 gttaacttcg agggcacacc ggatatgatc ggtaaattcg tcgatgtaga aattaccgac
     2761 gtctacccga actccctgcg cggaaaagtg gtgcgtactg aagatgaaat gggtctgcgc
     2821 gtggcagaaa caccggaatc ggtgattgcc cgtacccgca aagaaaacga ccttggcgtg
     2881 ggttattatc agccgtaatt ctcaggcccg ccgttccggt gggccttgtt ttttctctct
     2941 ttatccccat ctttatcgca atgcttgcgc ctttttcctg tggcgtgaat aatttcctta
     3001 taggccggtg aatgttcagc ttccggcggc atacagataa gaggaacggt ttgaacatag
     3061 acactcgcga aatcactctg gagccagcag acaacgcacg tctgttgagc ctgtgcggcc
     3121 cgtttgatga caacatcaaa caactcgaac gccgtctcgg catcgagatc aatcgccgcg
     3181 ataaccactt taaactgacc ggccgtccga tttgcgtcac cgctgcggca gacattctgc
     3241 gtagcctgta tgtcgatact gccccgatgc gcggtcagat tcaggatatc gaaccggaac
     3301 agatccacct tgcgattaaa gaagcgcggg tactggagca aagcgcggag agcgtgccgg
     3361 agtatggcaa agcggtcaat atcaaaacca aacgcggcgt aattaagccg cgcacgccaa
     3421 accaggcgca gtacatcgcc aatattctcg accatgacat taccttcggc gttggcccgg
     3481 cgggtacggg taaaacctac ctggcagtgg ctgcggcagt tgatgccctg gagcgtcagg
     3541 aaattcgccg tattctgctg actcgtccgg cggtcgaagc cggtgagaaa ctgggcttcc
     3601 tgcctggcga tttaagccag aaagtagacc cgtatctgcg cccgctgtac gacgcgctgt
     3661 ttgaaatgct gggctttgag aaagtcgaga aactgattga gcgcaacgtt attgaagtcg
     3721 caccgctggc ctatatgcgt ggtcgtacgc tgaacgacgc gtttatcatt cttgatgaaa
     3781 gccagaacac caccatcgaa cagatgaaga tgttcctgac ccgtatcggt tttaactcaa
     3841 aagcggttat caccggcgac gtcacacaga tcgacctgcc gcgtaatacc aagtctggtt
     3901 tgcgtcatgc catcgaagtg ctggccgatg tcgaagagat cagctttaac ttcttccaca
     3961 gcgaagacgt ggttcgtcac ccggtggtgg cgcgtatcgt taacgcctat gaagcctggg
     4021 aagaagccga acaaaaacgt aaagcggcgc tggcagcaga acgcaagcgc gaagaacagg
     4081 aacaaaaatg agtcaggtga tcctcgattt acaactggca tgtgaagata attccgggtt
     4141 accggaagag agccagtttc agacatggct gaatgcggtg atcccgcagt ttcaggaaga
     4201 atcggaagtg actattcgcg tggtcgatac cgccgaaagc cacagtctga atctgaccta
     4261 tcgcggtaag gataagccga ccaacgtgct ctccttcccg tttgaagtac cgcctggcat
     4321 ggaaatgtcg ctactgggcg atctggttat ctgccgtcag gtggttgaga aggaagctca
     4381 ggagcaaggc aaaccactgg aggcgcactg ggcgcatatg gtggtgcacg gcagtctgca
     4441 tttgttaggt tacgatcaca tcgaagatga cgaagcagaa gaaatggaag ccatcgaaac
     4501 agagattatg cttgctctgg gctatgagga tccgtacatt gccgagaaag aataatttgc
     4561 caggcatttg actggcagac gtccccgccg ttggtgatta acgacggcgt tttgattaac
     4621 taacatgaga acccatacga cgccatgagc gacgacaatt cacacagtag tgacacgata
     4681 agcaacaaga agggattttt ctccctgtta ctcagccaac ttttccacgg tgaaccgaaa
     4741 aaccgtgacg aactgctggc gctgatccgt gattccgggc agaacgacct tatcgacgaa
     4801 gatacgcgcg atatgctcga aggggtgatg gacatcgcag accaacgcgt tcgcgacatc
     4861 atgatccccc gctcccagat gattaccctg aaacgcaacc agacgctgga cgaatgcctt
     4921 gatgtcatca ttgagtccgc ccactcacgt ttcccggtga ttagcgaaga caaagatcac
     4981 attgaaggga ttctgatggc gaaagacttg ctgccgttta tgcgcagcga tgctgaagcc
     5041 ttcagcatgg acaaagtgtt acgtcaggcg gttgtcgttc ctgaaagtaa gcgcgtagac
     5101 cggatgctga aagagtttcg ctctcagcgt taccacatgg cgatcgtcat tgacgaattc
     5161 ggtggggttt ccggtctggt gaccattgaa gacatcctgg aactgattgt tggtgagatt
     5221 gaagacgagt atgacgaaga agatgatatc gacttccgtc agctgagtcg tcatacttgg
     5281 accgtgcgcg cactggcttc tattgaagac ttcaacgaag cgttcggcac ccactttagc
     5341 gatgaagagg ttgacactat cggtggtctg gtgatgcagg catttgggca tcttccggcg
     5401 cgtggcgaaa ccatcgacat cgacggttac cagttcaaag tggcgatggc cgacagtcgg
     5461 cgtattattc aggttcatgt caaaatcccg gatgactcac cccagccgaa gctggatgaa
     5521 taaaaccgaa actggataga taactacatg gcttttgcct cattaattga acgccagcgc
     5581 attcgcctgc tgctggcgtt attattcggt gcctgcggaa cgctggcctt ctctccttac
     5641 gacgtctggc ctgcggcgat tatttcgctg atggggcttc aggcgttgac ctttaaccgc
     5701 cgtccactcc agtctgccgc tattggcttt tgctggggat ttggcctctt tggcagcggt
     5761 attaactggg tctatgtcag catcgcgacc tttggcggaa tgcctggccc ggttaacatc
     5821 ttcctggtgg tactgctggc ggcgtatttg tcgctgtata ccggactgtt tgctggcgtg
     5881 ctgtcgcgtc tgtggccgaa aaccacctgg ctgcgcgtgg cgattgccgc ccctgctctc
     5941 tggcaagtga ccgagtttct gcgcggttgg gtactaacag gcttcccgtg gttacagttc
     6001 ggctatagcc agattgatgg cccgttaaaa gggctggcac cgataatggg cgtggaagcc
     6061 attaacttcc tgctgatgat ggttagcggc ctgctggcac tggcgttggt caaacgcaac
     6121 tggcgtccgc tggtggtggc cgtcgtgctg tttgcccttc ccttcccgct gcgttacatc
     6181 cagtggttta ccccgcaacc ggaaaaaact attcaggttt cgatggttca gggcgatatt
     6241 ccgcaatcgc tgaaatggga cggagaccag ctacttaata cgctgaagat ttactacaac
     6301 gcaacggcac cgctgatggg caaatcatcg ctgattatct ggccggagtc ggcgataacc
     6361 gatctggaaa ttaatcagca accgttcctc aaagctctgg acggtgagtt gcgcgataaa
     6421 ggtagctcgc tggtcaccgg gattgtcgac gcgcgtctca ataaacagaa ccgctatgat
     6481 acctacaaca ccatcatcac actgggtaaa ggcgcaccgt acagctatga atcagccgat
     6541 cgctataaca aaaaccatct ggtgccgttt ggcgagtttg tcccactgga gtcgattctg
     6601 cgtccgttag cgccgttctt tgatctgccg atgtcgtcct tcagccgtgg gtcatatatc
     6661 cagccgccgc tgtcggtaaa tggtattgag cttactgcgg ctatttgcta cgagatcatt
     6721 cttggcgagc aagtgcgcga taacttccgc ccggataccg actatctgct gactatctcc
     6781 aacgatgcgt ggtttggtaa gtctattggt ccatggcagc acttccagat ggcgcgaatg
     6841 cgtgcgctgg agctggcgcg cccactgttg cgcagcacca acaacggcat tacggcggtg
     6901 attggcccgc agggtgagat tcaggcgatg atcccgcagt tcacccgcga ggtgttaacc
     6961 actaacgtaa cgccgactac cggactcaca ccgtacgcac gtaccggcaa ctggccgctg
     7021 tgggtgctga cggcactgtt tggttttgca gctgtgctga tgagtctgcg tcagcgacgt
     7081 aaacgttgat gtgaatgatg cgctggcctg caaaattgca tcaattttgc aggccagata
     7141 aaccgttcac accacatccg gtatcaacaa aacatacttt ctcattgagt tgtggggcat
     7201 gattatcatg cccctttttc ttacggttca gggatacaag ccacatcgcc atattgggga
     7261 tgacgaataa ttgcctgcgg atcaagtgaa ctttctctgt ccaattgtat tagcccgttc
     7321 ttcgccataa caaatatctt tctaccatcc gctgaaaaac gaagattatt aacgtagctg
     7381 gcaggataaa cgccaataat ctgcccggca ttaatattga gcaggatgac ataaccttgc
     7441 aggccatatg acaccaacgc taaatactga ccgtccgggc taataccctc aacggaataa
     7501 acgctcctgc tacaccattg cgtcataatg ctggcttgtt gatcctcagc tcttatacgt
     7561 gaaatcccct ggctatcggc aaaaataacc tcaccatttt ttggtcggaa tcccatcgca
     7621 gaaacgacac cacgcgcatt acctgcggga aagtcttctt tctgatgcgt gatggtattg
     7681 atccgcacga tgctacctct gcctgctccc ggaaccagta atgttttttc atccggggaa
     7741 agtgccataa cccaggcgcc tggtttaccg ctcatccccg tcccgtcgta tttctccagc
     7801 gggatatccg ccacatgctg ataagccacc agatcaatcg ctgacacact attgctggtg
     7861 cgattcatca cccaggcttt tgtgccatca cgcgagagga taaggctgtc aggatgtgcg
     7921 ccggtggtaa tcgactgaat aatctccccc gtcgccacgt taatgaccga taacgcattc
     7981 gcttcaaatg aagtcgcata aatcaacttt tcatccaggc ttaatgccag cgatctgacg
     8041 ccctgatctt tacagccacc acaatgtttg actgactttt ctttcgctat gggcgctgta
     8101 aaggtatgca ggattttctt ttgtccgagg tcgaaaacaa caatgctgtt cgtgttttca
     8161 attgcggcat acaacgtatt accatcccga ctgatcgcca tatcatggat atcggcaatc
     8221 tcagcgagta aatcaccact ggcctgctca cgacttaccg gagcggataa tgtttccctg
     8281 agggcgttgc gctcctccac taactgctgc tttttttgtc gtcgctcttg ttcaatctcc
     8341 tgcggataaa acgcttcacg caggctttgc ctgacctgta aaactgattc atcaatctgc
     8401 tgctgcacaa gccagatagc cccggtcaga agaagagtga ccgcaacaat aatcccgcct
     8461 tccgtaacga atcggttttg cccaactaaa cgcgcgctca gccaacccaa cgcgcccccc
     8521 gcaatcaggc cgccaatgtg gcaagcgtta tctattcctg tttgccgggt accatacaac
     8581 agtgtcagcg ccaccatacc caacagagga aatactcgcc gctggttttt atggtgtgtg
     8641 cccgcacctg aagcaagctg tgttgctatc gcagcgcccg ctattcccat aattgccccg
     8701 gatgccccaa cgctgatgac tatctgctca ctttcgcgta acgcataaca ggcgctgatc
     8761 agcccaccaa agatgccgga gaataaccag ataatcagga gtcgccattt ccccagtatc
     8821 cgttccgcca ctgtccccac caccaggagt gcaaacatat tcatcagcaa atgggaaaaa
     8881 ctactgtgca gaaaaacact gctcaataag cgccaggatt catccgacaa cgtgagagaa
     8941 gcaacgttag cgccgagatg gatgagtgcg gattgatcga cttcttcgta actcatccat
     9001 tgtgcgaggt aacaaaatat cccaacacag agcgcaaaaa ggatcagggt tattgcgggt
     9061 aattgaacat ttaacggctt aaccgaagat gcagacataa ggtgattctt ccttgaaaag
     9121 actagtaaaa agcattttcc cgtgtgtagt tatcatcccg gcaaacgtcc cacaccgaaa
     9181 agttaacact gtctgaaata tgactttttc aaactgaaat tacaatcgta attaattcac
     9241 ccctaagtat tttttggcat gtaagttgct ttatttactg tgtaagcaat gaaatcagcc
     9301 gcaacgcaac cgggttgcac caccacaaat cagcggtagc accaaaaagg tgcggtttaa
     9361 gaattttgct tcggaatggt gcggcgcacc tcgcaaaaat aaacaataaa acagcaatat
     9421 ctttacattt atcaaaacta aatgttacct aacaaacaca acactgcaca ataaagtcgc
     9481 agatgataac aacacaaaca ctcacaacgg gtatccatgc gtttttaacg cagaagataa
     9541 aggagttgga tatgcaatta cgtaaacctg ccacagcaat cctggccctg gcgctttccg
     9601 caggactggc acaggcagat gacgccgccc cggcagcagg cagcacgctg gacaaaatcg
     9661 ccaaaaacgg cgtgattgtc gtcggtcacc gtgaatcttc agtacctttc tcttattacg
     9721 acaatcagca aaaagtggtg ggctactcgc aggattactc caacgccatt gttgaagcag
     9781 tgaaaaagaa actcaacaaa ccagacttac aggtgaaatt gattccgatc acctcacaaa
     9841 accgtattcc actgctgcaa aacggcactt tcgattttga atgcggttct accaccaaca
     9901 atgccgaacg ccaaaaacag gcggctttct ctgacactat tttcgtggtc ggtacgcgcc
     9961 tgctgaccaa aaagggcggc gatatcaaag attttgccga cctgaaaggc aaagccgtag
    10021 tcgtcacttc cggcaccacc tctgaagttt tgctcaacaa actgaatgaa gagcaaaaaa
    10081 tgaatatgcg catcatcagc gccaaagatc acggtgactc tttccgtacc ctggaaagcg
    10141 gtcgtgccgt tgcctttatg atggatgatg ctctgctggc cggtgaacgt gcgaaagcga
    10201 agaaaccaga caactgggaa atcgtcggca agccgcagtc tcaggaagcc tacggttgca
    10261 tgttgcgtaa agacgatccg cagttcaaaa agctgatgga tgacaccatc gctcaggtgc
    10321 agacctccgg tgaagcggaa aaatggtttg ataagtggtt caaaaatcca attccgccga
    10381 aaaacctgaa catgaatttc gaactgtcag acgaaatgaa agcactgttc aaagaaccga
    10441 atgacaaggc actgaactaa ttacaagaac caggggcgga aattccagcc ctctcgattg
    10501 ttacgtagca cggacagact atacgcctgg tggtcgttcc ccatcgggcc tgaaaaccgc
    10561 aatacgctgg gtaacaatct tcgagggtag cagttaacgc tgctaccctc ttttttctgg
    10621 agtagattta tgtctataga ctggaactgg ggtatttttt tacaacaagc cccgttcggc
    10681 aacaccacct atcttggttg gatctggagt ggctttcagg tgacgatcgc tttgtcgatc
    10741 tgcgcctgga ttatcgcttt cctcgtcggt tcatttttcg gcattttacg taccgttcca
    10801 aaccgttttc tctccggtct gggtacgttg tatgtcgaac tgttccgcaa cgtgccgttg
    10861 attgtgcaat tctttacctg gtatctggtg atcccggagc tgctgccaga gaaaatcggc
    10921 atgtggttta aggcggaact ggaccctaat attcaatttt tcctttcatc catgctctgc
    10981 ctggggctgt ttaccgccgc ccgtgtttgc gaacaggtac gcgccgctat tcagtcgctg
    11041 ccgcgtgggc aaaaaaatgc cgcgctggcg atgggtctga cgctaccgca ggcttaccgc
    11101 tacgtactgc tgcctaatgc ttatcgcgtt atcgtcccgc cgatgacctc agagatgatg
    11161 aacctggtga aaaactccgc catcgcctcg actatcggtc tggtggatat ggcggcgcag
    11221 gcgggtaaat tgctcgatta ctcggcccac gcctgggagt cgtttaccgc cattacgctg
    11281 gcgtatgtat tgattaacgc ttttatcatg ctggtgatga cgctggttga acgtaaagtt
    11341 cgcctgcccg gcaacatggg gggcaaataa tgtacgagtt tgactggagt tccattgttc
    11401 cttccctgcc atatctgctc gacgggctgg tgatcacgct gaaaatcacc gtcacggcag
    11461 tggtgattgg tattttgtgg ggcaccatgc tggcggtgat gcgcttgtcc agctttgcgc
    11521 ccgtagcgtg gtttgccaaa gcctacgtta acgtattccg ctcgattcct ttagttatgg
    11581 ttttgctgtg gttttacctg atcgtaccgg gttttctgca aaacgtgctg ggattatcac
    11641 ccaaaaatga tatccgttta atttcggcga tggtggcgtt ttcgatgttt gaagcggcgt
    11701 actattcaga gattatccgt gctggtattc aaagtatttc tcgtggtcag tcgagcgccg
    11761 cgctggcgtt gggaatgact cactggcagt cgatgaaact gattattctg ccgcaggcgt
    11821 tccgcgcgat ggtgccgctg ttgctcactc agggcatcgt actgtttcag gatacctcac
    11881 tggtgtatgt gttatctctg gccgatttct tccgtaccgc ctcaaccatt ggtgagcgtg
    11941 atggtacgca ggttgagatg atcctgtttg ccggatttgt ttatttcgtt attagcctta
    12001 gcgcgtcgtt gttggtcagc tacttgaaaa gaaggacagc ataatgatta ccctgaaaaa
    12061 tgtttcaaaa tggtatggtc actttcaggt gctgaccgac tgctcaaccg aagtgaaaaa
    12121 aggcgaagtg gtggtggttt gcggcccgtc tggttcaggc aaatcaacgc tgattaaaac
    12181 cgtcaacggc ctcgaaccgg tgcagcaagg tgaaatcacc gtcgatggca tcgtggttaa
    12241 cgacaagaaa accgatctgg cgaaactgcg ttcccgcgtc gggatggtgt ttcagcattt
    12301 cgagctgttc ccccatctgt cgattatcga aaacctgacc ctggcgcagg tgaaagtgct
    12361 taaacgcaat aaagcgccgg ctcgtgaaaa agcattgaaa ttgctggagc gcgtcgggct
    12421 ttccgcacat gccaataagt ttccggcgca gctttccggc ggccagcagc agcgtgtggc
    12481 gatcgctcgc gcattgtgca tggatcctat tgccatgctg ttcgacgaac caacgtcggc
    12541 gctggatccg gagatgatca acgaagtgct ggacgtgatg gtcgaactgg cgaacgaagg
    12601 aatgacgatg atggtggtga ctcacgaaat gggctttgcc cgtaaagtgg cgaatcgggt
    12661 gatctttatg gacgagggta aaattgtcga agactcgccg aaagacgctt tcttcgatga
    12721 tccgaaatcg gaccgcgcaa aagacttcct cgcaaaaatc ctgcattaat cactctggcg
    12781 cgcatactca attgcgcgcc atctcacgct ttctcgtttc gtccctcgta tctgttacat
    12841 cccggcgtta cccttgtcgc aaagaaacac acaacaagga gcaacaatgg cactgccaat
    12901 tctgttagat tgcgacccag gtcatgacga cgctatcgca atcgttctcg ccctcgcctc
    12961 accagagctt gatgtcaaag caattacgtc ttccgccgga aaccagacac cagaaaaaac
    13021 cttacgcaat gttctgcgta tgctgacctt gcttaatcgc accgatattc cggtagcaag
    13081 cggtgcggta aaaccgttaa tgcgtaattt gattattgcg gacaacgtgc acggcgaaag
    13141 cggtctcgac ggcccggcat taccggaacc ggcatttgca ccgcaaaact gcacggcagt
    13201 ggagctgatg gcgaaaacgc tgtgtgaaag tgaggaacct gtcaccattg tttctaccgg
    13261 accgcaaact aacgttgcct tgctgctcaa tagccacccg gaactgcata gcaaaattgc
    13321 ccgtatcgtg attatgggcg gcgcaatggg gcttggtaac tggacgcctg cggctgaatt
    13381 taatatttac gttgacccgg aagcggcaga aattgtcttc cagtcaggaa ttccggtggt
    13441 gatggccggt ctggatgtta ctcacaaagc acaaatccac gttgaagaca ctgagcgttt
    13501 ccgcgcgatt ggcaaccctg tttcaaccat tgttgccgaa ctgctggatt tcttcctcga
    13561 atatcacaaa gacgaaaaat ggggctttgt cggcgcacca ctgcatgacc catgcaccat
    13621 cgcctggctg ttgaaaccgg agttgtttac cactgttgag cgctgggttg gcgtggaaac
    13681 acaagggaaa tatacccagg gtatgacggt tgttgattat tattatctga ctggcaataa
    13741 accgaatgcc accgtaatgg tcgatgttga tcgtcagggc tttgttgatt tactggctga
    13801 tcgtctgaaa ttttacgctt aatcactcgc cggatgacat cacgccatcc ggcaccactc
    13861 atttcattac ggctcgaacg gacgtctctg gaactgatca tgaccacatt tcgggcatag
    13921 cgtcagcact tccggtgtat agatcgggag atggaagtga catttctcgc agaccagatt
    13981 tcccagccct accacttctc cgctgtgata aaccccatga tgattgaggt cctggaaaac
    14041 ttcgcgccat tcaagctgcg ttttatcggt gatgtctgcc agctcctgcc acaagctttc
    14101 tttaatcacc cgcataaaga cgctgtcaga ctcttctttc aggctctctt catagctcat
    14161 ggcgaactct tccaggtcac gtctgacagc tcgcgtcagc tcatcgacct cggttcgcgt
    14221 taactcccct gtttttatta cgcgctcgcg cgcctgttcc accagtgcgt cgatatcacg
    14281 ttcgccattg cgcaggcgtt cgctcagtga cgcgaccagt tcacggtaat attgagcaac
    14341 cttgttcatc gtttcatctc ccgggtcgtt aactgactat aataatagac gttctttgtt
    14401 ccttgctttg ctaatacgcc cacaaactct gtaaattaat gtatgaataa tccgggcatc
    14461 atttcaacgt cgagtgcgcg aaaggctgtt ttgacgcggg cgtttgggct atgctatgcg
    14521 gatctgaaaa accacatcaa cgctacattt gtagccgtat tgaaaacagg accactggct
    14581 gccatgcaag agcaataccg cccggaagag atagaatcca aagtacagct tcactgggat
    14641 gagaagcgca catttgaagt aaccgaagac gagagcaaag agaagtatta ctgcctgtct
    14701 atgcttccct atccttctgg tcgactacac atgggccacg ttcgcaacta caccatcggt
    14761 gacgtgatcg cccgctacca gcgcatgctg ggcaaaaacg ttcttcagcc aatcggctgg
    14821 gacgcatttg gtctgcctgc ggaaggcgcg gcggtgaaaa acaacaccgc gccagcaccg
    14881 tggacgtacg acaacatcgc gtatatgaaa aaccagctca aaatgctggg ctttggttat
    14941 gactggagcc gcgagctggc gacctgtacg ccggaatact accgttggga acagaaattc
    15001 ttcaccgaac tgtataaaaa aggcctggta tataagaaga cttctgcggt caactggtgc
    15061 ccgaacgacc agaccgtact ggcgaacgaa caagttatcg acggctgctg ctggcgctgc
    15121 gataccaaag ttgagcgtaa agagatcccg cagtggttta tcaaaatcac tgcttacgct
    15181 gacgagctgc tcaacgatct ggataaactg gatcactggc cagacaccgt taaaacaatg
    15241 cagcgtaact ggatcggtcg ttccgaaggc gtggaaatca ccttcaacgt taacgactat
    15301 gacaacacgc tgaccgttta cactacccgc ccggacacct ttatgggttg tacctacctg
    15361 gcggtggctg cgggtcatcc gctggcacag aaagcggcgg aaaataatcc tgaactggcc
    15421 gcctttattg acgaatgccg taacaccaaa gttgccgaag ctgaaatggc gacgatggag
    15481 aaaaaaggcg tcgatactgg ctttaaagcg gttcacccat taacgggcga agaaattccc
    15541 gtttgggcag caaacttcgt attgatggaa tacggcacgg gcgcagttat ggcagttccg
    15601 ggtcacgacc agcgcgacta cgagtttgcc tctaaatacg gcctgaacat caaaccggtt
    15661 atcctggcag ctgacggctc tgagccagat ctttctcagc aagccctgac tgaaaaaggc
    15721 gtgctgttca actctggcga gtttaatggt cttgaccatg aagcggcctt caacgccatc
    15781 gccgataaac tgactgcgat gggcgttggc gagcgtaaag tgaactaccg cctgcgcgac
    15841 tggggtgttt ctcgtcagcg ttactggggc gcgccgattc cgatggtgac gctggaagac
    15901 ggtaccgtaa tgccgacccc ggacgaccag ctgccggtga tcctgccgga agatgtggtg
    15961 atggacggca ttaccagccc gattaaagca gatccggagt gggcaaaaac taccgttaac
    16021 ggtatgccag cactgcgtga aaccgacact ttcgacacct ttatggagtc ctcctggtac
    16081 tatgcgcgct acacttgccc ggagtacaaa gaaggtatgc tggattccaa agcggctaac
    16141 tattggctgc cggtggatat ttacattggt ggtatcgaac acgccattat gcacctgctc
    16201 tacttccgct tcttccacaa actgatgcgc gatgcaggca tggtgaactc tgacgaacca
    16261 gcgaaacagt tgctgtgtca gggtatggtg ctggcagatg ccttctacta tgttggtgaa
    16321 aacggcgaac gtaactgggt ttccccggtt gatgctatcg ttgaacgtga cgagaaaggc
    16381 cgtatcgtga aagcgaaaga tgcggcaggc catgaactgg tttataccgg catgagcaaa
    16441 atgtccaagt cgaagaacaa cggtatcgac ccgcaggtga tggttgaacg ttacggcgcg
    16501 gacaccgttc gtctgtttat gatgtttgct tctccggctg atatgactct cgaatggcag
    16561 gaatccggcg tggaaggggc taaccgcttc ctgaaacgtg tctggaaact ggtgtacgag
    16621 cacacagcaa aaggtgatgt tgcggcactg aacgtcgatg cgctgactga agatcagaaa
    16681 gcgctgcgtc gcgatgtgca taaaactatc gctaaagtaa ccgatgatat cggccgtcgt
    16741 cagaccttca acaccgcaat tgcggcgatt atggagctga tgaacaaact ggcgaaagca
    16801 ccgaccgatg gcgagcagga tcgcgccctg atgcaggaag cgctgctggc ggtagtccgt
    16861 atgcttaacc cgttcacccc gcacatctgc ttcacgctgt ggcaggaact gaaaggcgaa
    16921 ggcgatatcg acaacgcgcc gtggccggtt gctgacgaaa aagcgatggt ggaagactcc
    16981 acgctggtcg tggtgcaggt taacggtaaa gtccgtgcaa aaatcaccgt tccggtggac
    17041 gcaacggaag aacaggttcg cgaacgtgct ggccaggaac atctggtagc aaaatatctt
    17101 gatggcgtta ctgtacgtaa agtgatttac gtaccaggta aactcctcaa tctggtcgtt
    17161 ggctaagcgc gggaggaagc gtgcgatatc tggcaacatt gttgttatct ctggcggtgt
    17221 taatcaccgc cgggtgtggc tggcatctgc gtgacaccac gcaggttcct tccactatga
    17281 aagtcatgat cctggactca ggcgatccga acgggccatt aagccgtgcg gtgcgtaatc
    17341 agttacgtct gaatggtgtc gagttgcttg ataaagaaac cacgcgtaag gacgttccat
    17401 ccctgcgttt gggtaaagtg agcatcgcga aagataccgc atcggtattc cgtaacggtc
    17461 aaacagcaga gtatcagatg atcatgacgg tgaatgcgac cgtgttgatc cccggccgtg
    17521 atatctaccc gattagcgcc aaagtcttcc gttcgttctt cgataacccg caaatggcgt
    17581 tagcgaaaga taacgaacaa gacatgatcg taaaagagat gtacgaccgt gctgccgaac
    17641 agctgattcg taagctgcca agcatccgtg ctgcggatat tcgttccgac gaagaacaga
    17701 cgtcgaccac aacggatact ccggcaacgc ctgcacgcgt ctccaccacg ctgggtaact
    17761 gatgattcgg ttgtacccgg aacaactccg cgcgcagctc aatgaagggc tgcgcgcggc
    17821 gtatctttta cttggtaacg atcctctgtt attgcaggaa agccaggacg ctgttcgtca
    17881 ggtcgctgcg gcgcaagggt tcgaagaaca ccacactttt tccattgatc ccaacaccga
    17941 ctggaatgcg attttttcgt tatgccaggc catgagcctg tttgccagtc gacaaacgct
    18001 attgctgttg ttacccgaaa acggaccgaa tgcggcgatc aatgaacaac ttctcacact
    18061 caccggactt ctgcatgacg atctactgtt gatcgtccgc ggtaataaat taagcaaagc
    18121 gcaagaaaat gccgcctggt ttactgctct tgcgaatcgc agcgtgcagg tgacctgtca
    18181 gacaccggag caggctcagc tgccccgctg ggttgctgcg cgcgcaaaac agctcaactt
    18241 agaactggat gacgctgcaa atcaggtgct ctgctactgt tatgaaggta acctgctggc
    18301 gctggcccag gcactggagc gtttatcgct actctggccg gacggcaaac tgacattacc
    18361 gcgcgttgaa caggcggtga atgatgccgc gcatttcacc ccttttcatt gggttgatgc
    18421 tttgttgatg ggaaaaagta agcgcgcgtt gcatattctt cagcaactgc gtctggaagg
    18481 cagcgaaccg gttattttgt tgcgcacatt acaacgtgaa ctgttgttac tggttaacct
    18541 gaaacgccag tctgcccata cgccactgcg tgcgttgttt gataagcatc gggtatggca
    18601 gaaccgccgg ggcatgatga gcgaggcgtt aaatcgctta agccagtcgc agttacgtca
    18661 ggccgtgcaa ctcctgacac gaacggaact caccctcaaa caagattacg gtcagtcagt
    18721 gtgggcagag ctggaagggt tatctcttct gttgtgccat aaacccctgg cggacgtatt
    18781 tatcgacggt tgatatgaaa tctttacagg ctctgtttgg cggcaccttt gatccggtgc
    18841 actatggtca tcttaaaccc gtggaaacgc tggcgaattt gattggtctg acgcgggtca
    18901 caatcatccc taataatgtt cctccgcatc gtccccagcc ggaagcaaac agcatgcagc
    18961 gtaaacacat gcttgaactg gcgattgccg acaagccatt atttactctt gatgaacgcg
    19021 agctaaagcg caatgccccc tcttacactg cgcaaacact gaaagagtgg cggcaggagc
    19081 aaggaccggg cgtgccgctg gcgtttatta ttggtcagga ttcattgctg acctttccga
    19141 cctggtacga atacgaaacg atactcgaca atgcacattt gatcgtctgt cggcgtcctg
    19201 gttacccact tgaaatggca caaccacaat atcagcaatg gctggaggat catttgacac
    19261 ataacccgga agatcttcac cttcagcctg ccggtaaaat ttatctggct gaaacgccgt
    19321 ggtttaacat ctcggcgacc atcatccgcg aacgtttgca aaatggtgaa tcgtgtgagg
    19381 atttattacc ggaaccggta ctgacttaca ttaaccaaca aggcttgtat cgctgatacc
    19441 tgtcgttacg gagggcatga tgcgactgtg gttaattcgt catggtgaaa cgcaagcgaa
    19501 tgtcgatggt ctgtacagcg gtcatgcgcc cacacccctg accgcgcgcg gtattgagca
    19561 agcgcaaaat ctgcatacgc tgctacatga tgtttccttt gacctggttt tatgcagtga
    19621 actggaacgg gcacagcata ccgcgcgacg ggttctcagt gaccgccagc accccgttca
    19681 tatcatccct gaactcaatg aaatgttttt tggcgactgg gagatgcgac atcatcgtga
    19741 cctcatgcaa gaagatgccg aaaactatag cgcgtggtgc aatgactggc agcatgctat
    19801 ccccacgaac ggtgaaggat ttcaggcatt ttcgcaacgt gtggaacgat ttatcgcaag
    19861 gcttagtgaa tatcagcact atcagaatat tttagtcgtc agccatcagg gcgtactgag
    19921 tctgttaatt gctcgtttaa ttggcatgcc tgccgagtct atgtggcatt tccgcgttga
    19981 ccaggggtgt tggagcgcca ttgatatcaa ccaaaaattc gcgacgctac gcgtcctcaa
    20041 tagccgtgcc atctgggtcg agaatgcatg acttttctgt ttttttacgg gtaagccgca
    20101 acgaccattg acagccccgg gcaggctgat attctccgca gccagacttt ttccgccaga
    20161 cacgactttg tagaaattgt tttacaaaaa tggcgatgca atctgcggcg cggggtggga
    20221 tgatagccca ctttcgaaag ccgattcggc gacaattgtc ccgaaatcgc ctctggttca
    20281 ggtatactga cagaccattt ttatctattt gattcaccca gggggaaaac ttgcagggta
    20341 aagcactcca ggattttgtt atcgacaaaa ttgatgacct caaaggtcag gacatcatcg
    20401 ccttagacgt tcagggcaaa tccagcatca ccgactgcat gatcatctgt acgggtacgt
    20461 ccagccgcca tgttatgtcc attgctgatc acgttgtgca ggagtctcgc gcagcgggcc
    20521 tgttaccgct cggcgtagaa ggtgaaaaca gcgccgactg gattgtcgtg gatttgggcg
    20581 atgtgattgt ccatgtcatg caggaagaga gccgtcgcct gtatgaactg gaaaaactct
    20641 ggagttaatg cgtgaagctg caacttgtcg ccgtgggaac gaaaatgccg gactgggtac
    20701 aaaccggttt taccgagtac ctgcgtcgtt ttccgaaaga tatgcccttc gagctgattg
    20761 aaattccggc cggaaaacgc ggcaagaatg cggacatcaa gcgcatactc gacaaagagg
    20821 gtgagcagat gttggcggcc gcaggcaaaa accgcattgt caccctcgat attccaggca
    20881 agccctggga tacgccgcag ttagccgctg agctggaacg ctggaagctg gatggtcgcg
    20941 acgtcagtct actgattggc gggcctgaag ggttgtcgcc tgcctgtaaa gcggcggcgg
    21001 agcagagctg gtcgctgtcg gcacttaccc ttccccatcc gctggttcgc gtgctggtcg
    21061 cagagagtct gtaccgggcg tggagcatca ccaccaacca tccttatcac cgtgagtgat
    21121 aagtgagctt tgagtagaaa acgcagcgga tgaaactaca gaactctttt cgcgactata
    21181 cggcagagtc cgcgctgttt gtgcgccggg cgctggtcgc ctttttgggg attttgctgc
    21241 tgaccggcgt gcttatcgcc aacctgtata atctgcaaat tgttcgcttt accgactacc
    21301 agacccgctc taatgaaaac cgcattaagc tggtgcctat cgcacccagc cgcggcatta
    21361 tctacgaccg taacggtatc cctctggctc tcaaccgcac tatctaccag atagaaatga
    21421 tgccggagaa agtcgataac gtgcagcaaa cgctggacgc tttgcgcagc gtggttgatc
    21481 tgaccgatga tgatattgct gcattccgaa aagagcgcgc acgttcacac cgtttcacct
    21541 ctattccggt gaaaactaac ctgaccgaag tacaagtagc tcgctttgcc gtcaaccagt
    21601 accgttttcc gggtgttgaa gttaaaggct ataaacgtcg ttactatcct tacggttcgg
    21661 cgttgaccca cgtcatcggc tatgtgtcga aaatcaacga taaagacgtc gaacgcctga
    21721 ataatgacgg caaactggcc aactatgcgg caacgcatga tatcggtaag ctgggcattg
    21781 agcgttacta tgaagacgtg ctgcacggtc agaccggtta tgaagaggtt gaagttaaca
    21841 accgtgggcg tgttatccgc cagttaaaag aagtaccacc gcaggccgga cacgatattt
    21901 acctgacgct ggatctcaaa ctccagcaat atattgaaac gctgctggcg ggtagccgcg
    21961 cagctgtggt agtcaccgat ccgcgtacag gtggggtgtt ggcgctggtt tccacgccta
    22021 gttatgaccc aaacctgttt gttgacggta tctccagcaa agattattcc gccttgctga
    22081 acgacccgaa tacaccgctg gtgaaccgcg ccacacaggg ggtttatcct cccgcgtcta
    22141 cagttaaacc ctatgttgcg gtttcggcat tgagtgccgg ggtgatcacg cgcaatacga
    22201 cgctgtttga ccccggctgg tggcaactgc cagggtcgga aaaacgttat cgtgactgga
    22261 aaaaatgggg ccacgggcgt ctgaatgtca caagatcgct ggaagaatct gcggatacct
    22321 tcttctatca ggtggcctac gatatgggga tcgatcgcct ctccgaatgg atggggaaat
    22381 tcggttatgg tcattacacc ggtatcgacc tggcggaaga acgttccggc aacatgccta
    22441 cccgcgaatg gaaacagaaa cgctttaaaa aaccgtggta tcagggtgac accattccgg
    22501 ttggtatcgg tcagggttac tggacagcga ccccaatcca gatgagtaag gcactgatga
    22561 tcctgattaa tgacggtatc gtgaaggttc cgcatttgct gatgagcacc gccgaagacg
    22621 gcaaacaggt gccatgggta caaccgcatg aaccgcccgt cggcgatatt cattccggtt
    22681 actgggagct ggcgaaagac ggtatgtacg gtgttgctaa ccgccctaac ggtacagcgc
    22741 ataaatactt tgccagcgca ccctacaaaa ttgcggcgaa atccggtacc gctcaggtct
    22801 tcggtctgaa agcgaacgaa acctataatg cgcacaaaat tgccgagcgt ttacgtgacc
    22861 acaaactgat gaccgccttt gcgccataca acaatccgca agtggctgtc gccatgattc
    22921 tggagaacgg cggcgcgggt ccggcggttg gtacgctgat gcgccagatc ctcgaccaca
    22981 ttatgctggg tgataacaac accgatctgc ctgcggaaaa tccagcggtt gccgcagcgg
    23041 aggaccatta atcatgacgg ataatccgaa taaaaaaaca ttctgggata aagtccatct
    23101 cgatcccaca atgctgctga tcttactggc attgctggtt tacagcgccc tggttatctg
    23161 gagcgccagc ggtcaggata ttggcatgat ggagcgtaaa atcggccaga ttgcgatggg
    23221 tctggtcatc atggtggtga tggcgcaaat tcctccacgc gtttatgaag gctgggcccc
    23281 ctatctctat atcatctgta ttattttgct ggtggcggta gatgctttcg gtgccatctc
    23341 taaaggcgct caacgctggc tggacctcgg tattgttcgt tttcaaccgt cggaaattgc
    23401 caagatagcc gtaccgttga tggttgcacg ctttatcaac cgcgacgttt gcccaccttc
    23461 attgaagaat acagctatcg cgctggtgct gatatttatg cccacgctgc tggtggctgc
    23521 acagcctgac ttgggaacat caatcctcgt tgcgctttcc ggcctgtttg tactgttcct
    23581 ctctggcctt agctggcgtc tgattggcgt cgcagtagtg ctggtagcgg cgttcattcc
    23641 gattctgtgg ttcttcctga tgcatgatta ccagcgccag cgcgtaatga tgctcctgga
    23701 cccggaatca gacccactcg gcgcaggcta tcacattatt cagtctaaaa ttgctattgg
    23761 ctccggcgga ttacgcggca aaggctggct gcacggcact cagtcacagc ttgaatttct
    23821 ccccgaacgc cataccgact ttatcttcgc ggtactggcg gaagagctgg gattagtggg
    23881 cattttgatt ctgctcgctc tctacattct gctgatcatg cgcgggctgt ggatagccgc
    23941 cagagcgcaa accacctttg gtcgcgtcat ggctggcggc ttaatgctga tattattcgt
    24001 ttatgtcttc gtaaatattg gtatggtaag cggtattctg ccggttgtag gggttccgct
    24061 cccactggtc agttatggag gatcggcgct aattgtgctg atggctgggt tcgggattgt
    24121 aatgtcaatc cacacccaca ggaaaatgtt gtcgaaaagc gtgtaagagg tgcgcaatgc
    24181 gtaagcagtg gctcgggatc tgcatcgcgg caggaatgct cgcggcatgt acaagcgatg
    24241 atggtcagca acagacggta agtgtaccgc agcctgcggt atgtaacggc cccatagttg
    24301 aaattagcgg ggcagacccg cgtttcgaac cactgaacgc gacggcaaat caggattacc
    24361 agcgcgacgg taaaagctac aaaatcgtgc aggatccgtc tcgatttagc caggcgggac
    24421 tggcggcaat ctatgatgcc gaacccggca gtaacctgac ggcctctggc gaagctttcg
    24481 atccgacaca gttgacggcg gcccatccaa cgcttccaat ccccagctac gccagaatca
    24541 ctaacctggc taacgggcga atgatcgtgg tgcgcattaa tgatcgcggc ccttatggca
    24601 acgaccgcgt gatttcgctt tctcgcgcgg cagctgaccg tcttaacacg tcaaacaaca
    24661 ccaaagttcg tatcgatccg attattgtcg cccaggatgg ttcgctttct ggtcctggta
    24721 tggcttgtac cacagtcgcc aaacagactt acgccctgcc agcacctccc gatttaagcg
    24781 gtggcgcggg aacaagttca gtgcctgtcc cgcagggtga cattcttccg gtcagtaatt
    24841 cgacgctaaa aagcgaagat ccgaccggcg cgccggtaac cagcagcggt ttcctcggcg
    24901 caccaacgac cttagcgcct ggtgtactgg aaggcagcga accgacgcct gcgccacagc
    24961 ccgttgttac agctccgtcg acaacgcctg caacctcacc tgcaatggtg acaccgcaag
    25021 ccgcctcgca aagcgccagc ggcaacttta tggtgcaagt cggggccgtt agcgatcagg
    25081 ctcgtgcgca acagtaccaa cagcaactgg gacagaagtt cggcgtcccc ggtcgcgtaa
    25141 ctcaaaatgg cgcggtctgg cggatccagc ttggcccatt cgccagcaaa gccgaagcca
    25201 gtaccttgca gcaacgtttg caaaccgaag cccaattaca gtcatttatt accaccgcgc
    25261 agtagcgtaa agcaggcatc tgatgtgtca atttctgtaa atgacgttaa caaagtcacg
    25321 cggaaagtca gatgcctgcc ggtagtgcat ttgctatagt agggcacttt ttttaattcc
    25381 atcacggatg tcgtagttca gaccatgaat accatttttt ccgctcgtat catgaagcgc
    25441 ctggcgctca ccacggctct ttgcacagcc tttatctctg ctgcacatgc cgatgacctg
    25501 aatatcaaaa ccatgatccc gggtgtaccg cagatcgacg cggagtccta catcctgatt
    25561 gactacaact ccggcaaagt actcgccgaa cagaacgctg atgtccgccg cgatcctgcc
    25621 agcctgacca aaatgatgac cagttacgtt atcggccagg caatgaaagc cggtaaattt
    25681 aaagaaactg atttagtcac tatcggcaac gacgcatggg ccaccggtaa cccggtgttt
    25741 aaaggttctt cgctgatgtt cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc
    25801 cgcggtatta acctgcaatc gggtaacgat gcttgtgtcg ccatggccga ttttgccgct
    25861 ggtagccagg acgcttttgt tggcttgatg aacagctacg ttaacgccct gggcctgaaa
    25921 aatacccact tccagacggt acacggtctg gatgctgatg gtcagtacag ctccgcgcga
    25981 gatatggcgc tgatcggcca ggcgttgatc cgtgacgtac cgaatgaata ctcgatctat
    26041 aaagaaaaag aatttacgtt taacggtatt cgccagctga accgtaacgg cctgttatgg
    26101 gataacagcc tgaatgtcga cggcatcaaa accggacaca ctgacaaagc aggttacaac
    26161 cttgttgctt ctgcgactga aggccagatg cgcttgatct ctgcggtgat gggcggacgt
    26221 acttttaaag gccgtgaagc cgaaagtaaa aaattgctaa cctggggctt ccgtttcttc
    26281 gaaaccgtta atcctctgaa agtaggtaaa gagttcgcct ctgaaccggt ttggtttggt
    26341 gattctgatc gcgcttcgtt aggggttgat aaagacgtgt acctgaccat tccgcgtggt
    26401 cgcatgaaag atctgaaagc cagctatgtg ctgaacagca gtgaattgca tgcgccgctg
    26461 caaaagaatc aggtcgtcgg aactatcaac ttccagcttg atggcaaaac gatcgaacaa
    26521 cgcccgctgg ttgtgctgca agaaattccg gaaggtaact tcttcggcaa aatcattgat
    26581 tacattaaat taatgttcca tcactggttt ggttaaaaat tgaacacttg aaagtgtaat
    26641 ttccgtcccc atatactaag catcagtaaa aaactcccgc cttctggcgg gagttgctat
    26701 ttaattacgt tacgccggag ctgacatgaa aaccaaactt aacgaactgc ttgaattccc
    26761 tactcctttt acttacaaag ttatggggca ggcgttacct gagctggttg atcaggtggt
    26821 tgaagtggta cagcgccatg cgccaggtga ctacacccca acggtaaaac caagcagcaa
    26881 aggcaactac cactcggtat ctatcactat caacgccact catatcgagc aggttgaaac
    26941 actgtatgaa gaactgggca aaatcgatat tgtccgcatg gttctgtaac tcgcttctcc
    27001 gttacccggc tcctggtcgg gtaactcccc tctcctcgct gtgatatact tttcccccac
    27061 ttttactcat tctccacgga gatgccgttt tgtatcagga taaaattctt gtccgccagc
    27121 tcggtcttca gccttacgag ccaatctccc aggctatgca tgaattcacc gatacccgcg
    27181 atgatagtac ccttgatgaa atctggctgg tcgagcacta tccggtattc acccaagggc
    27241 aggcaggaaa ggcggagcat attttaatgc cgggtgatat tccggtgatc cagagcgatc
    27301 gcggtgggca ggtgacttat cacgggccgg ggcaacaggt gatgtatgtg ttgcttaacc
    27361 tgaaacgccg taaactcggt gtgcgtgaac tggtgacctt gcttgagcaa acagtggtga
    27421 ataccctggc tgaactgggt atagaagcgc atcctcgggc tgacgcgcct ggtgtctatg
    27481 tcggggaaaa gaaaatttgc tcactgggtt tacgtattcg acgcggttgt tcattccacg
    27541 gtctggcatt aaacgtcaat atggatcttt caccattttt acgtattaat ccttgtgggt
    27601 atgcaggaat ggaaatggct aaaatatcac aatggaaacc cgaagcgacg actaataata
    27661 ttgcaccacg tttactggaa aatattttag cgctactaaa caatcctgac ttcgaatata
    27721 ttaccgctta attccataca tcaatggccc aatttacact gggtcattac tcataatacc
    27781 tgctaatgct ttacatctcc ttcgcgtttg tatattttct ttatcacttg cagaaatgcg
    27841 atgctacgcc ttgctccgta gctcacccaa ttggttaact cattgaaata attaccatgg
    27901 tgagcaaaag cgttccgtcc caattagaca taagattat
//
LOCUS       DADSZD010000047        27857 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.047, whole genome shotgun sequence.
ACCESSION   DADSZD010000047 DADSZD010000000
VERSION     DADSZD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27857
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.047"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            232..735
                     /gene="dps"
                     /locus_tag="J1Z28_001802"
                     /gene_synonym="pexB"
     CDS             232..735
                     /gene="dps"
                     /locus_tag="J1Z28_001802"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBA7716642.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1139..1885
                     /gene="glnH"
                     /locus_tag="J1Z28_001803"
     CDS             1139..1885
                     /gene="glnH"
                     /locus_tag="J1Z28_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBA7716643.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2024..2683
                     /gene="glnP"
                     /locus_tag="J1Z28_001804"
     CDS             2024..2683
                     /gene="glnP"
                     /locus_tag="J1Z28_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBA7716644.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2680..3402
                     /gene="glnQ"
                     /locus_tag="J1Z28_001805"
     CDS             2680..3402
                     /gene="glnQ"
                     /locus_tag="J1Z28_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017491583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBA7716645.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3519..5744
                     /gene="ybiO"
                     /locus_tag="J1Z28_001806"
     CDS             3519..5744
                     /gene="ybiO"
                     /locus_tag="J1Z28_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBA7716646.1"
                     /translation="MRWILFILFYLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPLPPGEPT
                     L"
     gene            complement(5741..6667)
                     /gene="rlmF"
                     /locus_tag="J1Z28_001807"
     CDS             complement(5741..6667)
                     /gene="rlmF"
                     /locus_tag="J1Z28_001807"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBA7716647.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEATGSIS
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6943..7203
                     /locus_tag="J1Z28_001808"
     CDS             6943..7203
                     /locus_tag="J1Z28_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7716648.1"
                     /translation="MKKCLTLLIATVLSGISLTADAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTAIIYK"
     gene            7468..9750
                     /locus_tag="J1Z28_001809"
     CDS             7468..9750
                     /locus_tag="J1Z28_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HBA7716649.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGAG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSITDTATMRFEHDINDNATIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNIYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            9966..10643
                     /gene="ybiX"
                     /locus_tag="J1Z28_001810"
     CDS             9966..10643
                     /gene="ybiX"
                     /locus_tag="J1Z28_001810"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBA7716650.1"
                     /translation="MMYHIPGVLSPQDVARFREHLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            10717..10983
                     /gene="ybiI"
                     /locus_tag="J1Z28_001811"
     CDS             10717..10983
                     /gene="ybiI"
                     /locus_tag="J1Z28_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBA7716651.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQDKDLQKPAYTGYNRRGSKDSQLR"
     gene            11248..11508
                     /locus_tag="J1Z28_001812"
     CDS             11248..11508
                     /locus_tag="J1Z28_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7716652.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(11736..12821)
                     /locus_tag="J1Z28_001813"
     CDS             complement(11736..12821)
                     /locus_tag="J1Z28_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate/lactate/ureidoglycolate dehydrogenase"
                     /protein_id="HBA7716653.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVLPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(12962..13924)
                     /gene="ybiB"
                     /locus_tag="J1Z28_001814"
     CDS             complement(12962..13924)
                     /gene="ybiB"
                     /locus_tag="J1Z28_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBA7716654.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATINDGLARINQAF"
     gene            complement(13952..16102)
                     /gene="dinG"
                     /locus_tag="J1Z28_001815"
     CDS             complement(13952..16102)
                     /gene="dinG"
                     /locus_tag="J1Z28_001815"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBA7716655.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            16209..16406
                     /locus_tag="J1Z28_001816"
                     /pseudo
     CDS             16209..16406
                     /locus_tag="J1Z28_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1768 domain-containing protein"
     gene            complement(16642..18000)
                     /gene="rhlE"
                     /locus_tag="J1Z28_001817"
     CDS             complement(16642..18000)
                     /gene="rhlE"
                     /locus_tag="J1Z28_001817"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBA7716656.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AA"
     gene            18229..18900
                     /gene="cecR"
                     /locus_tag="J1Z28_001818"
     CDS             18229..18900
                     /gene="cecR"
                     /locus_tag="J1Z28_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBA7716657.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CKNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            18900..19898
                     /gene="hlyD"
                     /locus_tag="J1Z28_001819"
     CDS             18900..19898
                     /gene="hlyD"
                     /locus_tag="J1Z28_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBA7716658.1"
                     /translation="MMKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRT
                     VNLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLML
                     AGYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQA
                     TLKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLVAPSDGTLLTR
                     AVEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQ
                     IGFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            19891..21627
                     /locus_tag="J1Z28_001820"
     CDS             19891..21627
                     /locus_tag="J1Z28_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7716659.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDAALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFCL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            21620..22753
                     /locus_tag="J1Z28_001821"
     CDS             21620..22753
                     /locus_tag="J1Z28_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7716660.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLIVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            22764..23870
                     /locus_tag="J1Z28_001822"
     CDS             22764..23870
                     /locus_tag="J1Z28_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7716661.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(23832..24242)
                     /locus_tag="J1Z28_001823"
     CDS             complement(23832..24242)
                     /locus_tag="J1Z28_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716662.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQPWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            24375..25136
                     /locus_tag="J1Z28_001824"
     CDS             24375..25136
                     /locus_tag="J1Z28_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA7716663.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            25133..26374
                     /gene="clsB"
                     /locus_tag="J1Z28_001825"
     CDS             25133..26374
                     /gene="clsB"
                     /locus_tag="J1Z28_001825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBA7716664.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            26374..27330
                     /locus_tag="J1Z28_001826"
     CDS             26374..27330
                     /locus_tag="J1Z28_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBA7716665.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLVKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(27366..>27857)
                     /locus_tag="J1Z28_001827"
     CDS             complement(27366..>27857)
                     /locus_tag="J1Z28_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HBA7716666.1"
                     /translation="NKINTAIAGVIYLFLISLVAIVVASLAPVSAIIVVFSTAGSMFL
                     ISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERPIWVISCLMIVLWSGII
                     SHGRNKLLELTGKSHSEELWSSVRCAFIGALTLYYYFIGFFGILAAIAITLVWQRHTR
                     FFH"
BASE COUNT         6593 a         7384 c         7262 g         6618 t
ORIGIN      
        1 ttatatatcg aattatgcaa aagcatattt attccgaaaa ttcctggcga gcaaataaat
       61 aagaattgtt cttatcaata tatctaactt attgaatttt tattggtttt gtttttcacg
      121 cttgttacca ctattagtgt gataggaaca gccagaatag cggaacacat agctggtgct
      181 atacttagtc tcgttaatta ctgggacata acatcaagag gatatgaaat tatgagtacc
      241 gctaaattag ttaaatcaaa agcgaccaat ctgctttata cccgcaacga tgtctctgac
      301 agcgagaaaa aagcaacagt agagttgctg aatcgccagg ttatccagtt tattgatctt
      361 tctttgatta ccaaacaagc gcactggaac atgcgcggcg ctaacttcat tgccgtacat
      421 gaaatgctgg atggcttccg cactgcactg atcgatcatc tggataccat ggcagaacgt
      481 gcagtgcagc tgggcggtgt agctctgggg accactcaag ttatcaacag caaaactccg
      541 ctgaaaagtt acccgctgga catccacaac gttcaggatc acctgaaaga gctggctgac
      601 cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg gcgaagcgaa agatgacgac
      661 accgcagata tcctgaccgc cgcgtctcgc gacctggata aattcctgtg gtttatcgag
      721 tctaacatcg aataaatcca tcgctgatgg tgcagaactt tagtacacga taaaagcggc
      781 ttcctgacag gaggccgttt tgttttgtag cccgcctcaa cgcacttatt tagtgcatcc
      841 atctgctatc accagctggt taagtaaatt ttttgtatcc acatcatcac acaatcgtta
      901 cataaagatt gttttttcat caagttttac gctaaataat cactgtgttg agtgcacaat
      961 tttagcgcac cagtttggtg ccccagaatg gtgcatcttc agggtattgc cctataaatc
     1021 gtgcatcacg tttttgccgc atctcgaaaa atcaatgagt tgcaaaactg gcacgatttt
     1081 ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga tagaaaaaag gaaatgctat
     1141 gaagtctgta ttaaaagttt cactggctgc actgaccctg gcttttgcgg tttcttctca
     1201 tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc ttcgttccgt ttgaatttaa
     1261 acagggcgat aaatacgtgg gctttgacgt tgatctgtgg gctgccatcg ctaaagaact
     1321 gaagctggat tacgaactga agccgatgga tttcagtggg atcattccgg cactgcaaac
     1381 caaaaacgtc gatctggcgc tggcgggcat taccatcacc gacgagcgta aaaaagcgat
     1441 cgatttctct gacggctact acaaaagcgg cctgttagtg atggtgaaag ctaacaataa
     1501 cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt gctgtgaaga gcggcactgg
     1561 ctccgttgat tacgcgaaag caaacatcaa aactaaagat ctgcgtcagt tcccgaacat
     1621 cgataacgcc tatatggaac tgggcaccaa ccgcgcagac gccgttctgc acgatacgcc
     1681 aaacattctg tacttcatca aaaccgccgg taacggtcag ttcaaagcgg taggtgactc
     1741 tctggaagca cagcaatacg gtattgcgtt cccgaaaggt agcgacgagc tgcgtgacaa
     1801 agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact tacaacgaaa tctacaaaaa
     1861 atggttcggt actgaaccga aataataacg ctgcgcctgt aaagcgcact ggcagttccc
     1921 tctccccgcc ggggagagga ttagggtgaa gggcgcaaac ccgctctggg gccattaatt
     1981 accctgaatt tgattattta caccacggta acaggaacaa catatgcagt ttgactggag
     2041 tgccatctgg cctgccattc cgcttctgat tgaaggtgcc aaaatgaccc tgtggatttc
     2101 ggtcctcggt ctggctggcg gtctggtgat cggattgttg gcaggttttg cacgcacctt
     2161 cggtggttgg atagccaacc acgtcgcgct ggtctttatt gaagtgatcc gcggcacacc
     2221 tatcgtcgtc caggtgatgt ttatctattt cgccctgccg atggcgttta acgatttacg
     2281 catcgacccg tttactgcgg cggtggtcac catcatgatc aactccggcg cgtacattgc
     2341 ggaaatcacg cgtggtgcgg tgctgtctat tcacaaaggt tttcgtgaag caggactggc
     2401 gctcggtctt tcacgttggg aaaccattcg ctacgtcatt ttaccgctgg cactgcgtcg
     2461 tatgctgccg ccgctgggta accagtggat catcagcatt aaagacactt cgctgtttat
     2521 tgttatcggc gtggctgaac tgacccgtca ggggcaagaa attattgccg gtaacttccg
     2581 cgccctggag atctggagcg ccgtggcggt gttctatctg attattaccc tggtgctgag
     2641 ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg tgattgaatt taaaaacgtc
     2701 tccaagcatt ttggtccaac ccaggtgctg cacaatatcg atttgaacat tgctcagggc
     2761 gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat cgaccctgct gcgctgcatc
     2821 aacaaactgg aagaaatcac ttccggcgat ctgattgtcg atggcctgaa ggttaacgat
     2881 ccgaaagttg acgagcgcct gattcgccag gaagcaggta tggtgttcca gcagttttac
     2941 ctcttcccgc atctgacggc gctggaaaac gtcatgtttg gtccgctacg cgtgcgtggc
     3001 gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc tggcgaaagt cggtctggca
     3061 gaacgtgcac atcactaccc ttccgaactt tctggtggtc aacagcagcg tgtggcgatc
     3121 gcacgcgcgc tggcggtgaa gccgaaaatg atgctgtttg atgaaccgac ttccgctctt
     3181 gacccggaac tgcgccatga agtgctgaag gttatgcagg atctggctga agaagggatg
     3241 acgatggtga tcgtgacaca cgaaatcggt tttgccgaga aagtagcttc gcgcctgatc
     3301 tttatcgaca aaggccggat tgcggaagat ggcgatccgc aggtgttgat caagaaccca
     3361 ccgagccagc gcttgcagga atttttgcag cacgtctctt aacttctcag cctccctttc
     3421 ccccgaaagg gaggcacacc agattcctct catttaaaat cgcccctcct ccagcatcta
     3481 tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc ctgttcatcc
     3541 tcttctacct gctgggcgca cctgcccacg cggtatccat acccggcgtt acaaccacaa
     3601 cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa aaaaaagcgg
     3661 cctatggtgc actggcggat gtgctggata atgacacctc acgtaaagag ttgatcgacc
     3721 agttgcgcac cgtcgccgcg acgccccctg cggaacccgt accgaagatc gtgccgccga
     3781 cgctggttga agagcaaacc gtgctgcaaa aagtcaccga agtcagccgc cattatggtg
     3841 aagccctttc cgcccgcttc ggacaacttt atcgcaatat caccggctcc ccgcataagc
     3901 cgtttaatcc acaaaccttc agcaatgcgt tgacccattt ttcaatgtta gcggtattag
     3961 tgtttggttt ttactggctg attcgcctgt gcgcacttcc gctgtatcgc aaaatgggcc
     4021 agtgggcgcg gcaaaaaaat cgtgagcgca gtaactggtt gcagcttccg gcgatgatta
     4081 tcggggcgtt tattatcgac ctgctgttac tggcactgac attatttgtc ggtcaggttt
     4141 taagcgacaa cctgaatgcg ggcagtcgca ccatcgcttt ccaacaaagt ttgtttctca
     4201 acgcctttgc cctgattgaa tttttcaaag ctgtactacg cctgattttt tgcccaaacg
     4261 tggcggaact gcgcccgttc acgattcatg acgagagcgc ccgttactgg agccgtcgcc
     4321 tgagctggtt aagcagcctg attggctatg gcctgattgt ggccgtgccg attatctcta
     4381 atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg tgcatgacgg
     4441 tctgggcgct gtacctgatc tttcgtaata aaaaagagat tacccagcat ttgctcaact
     4501 tcgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg ctggtgtggc
     4561 actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc gatccgggca
     4621 acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt ggtatcgcag
     4681 catttgtttc cggtatgttt tcccgctggc tggcaaaaac catcaccctc tcgccacata
     4741 ctcagcgtaa ctatccagaa ctgcaaaaac ggctgaatgg ctggctttcg gcggcgctga
     4801 aaacggcgcg tattctgacc gtctgcgtgg cggtaatgtt gctgttgagc gcatggggat
     4861 tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta gatatcctga
     4921 tccgtatcgc actcattctt ttcttctcgg cggttggctg gacggtgctc gccagtttga
     4981 tcgaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc cgtacgcgaa
     5041 ccctgctgac gctgttccgt aacgcgctgg cggtgattat cagtaccatc accatcatga
     5101 ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc ggggcattag
     5161 gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga tatcattacc ggagtattta
     5221 ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg ttgaccggca
     5281 ctgtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg gcgtatcaca
     5341 tcattccgtg gtcttcgata accacctttg ctaacttcgt ccgcggtatt ggttcggtag
     5401 tggcaaatta tgatgttgat cgccatgaag atgctgataa agccaatcag gcactgaaag
     5461 atgcggtagc ggaattaatg gaaaacgaag aaattcgcgg gctgattatt ggtgaaccga
     5521 attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt tcgttcacca
     5581 cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg aaaaaacatt
     5641 tcgacctggc gggcgttcgc gcgccagtgc agacttatca ggtgctgcct gctccgggcg
     5701 cgaccccggc tgaaccgttg ccgccggggg aaccaacgct ttaacgctgg cgattgacaa
     5761 aacggcggcg ctgctcgtcg ttcataaagg tccaggctat aaagcggctc tgcttttgcc
     5821 cctgggccat ctcttttttt accaccttca ccgcacctac gtccgtcaga gcacgataca
     5881 acggcggtaa gttttcacca cgagatacca gcgaggtaaa ccacatcacc tgcttcgcga
     5941 agcctttgct ctcttcaatc atctttttga taaaagcgac ctcaccgcct tcacaccaca
     6001 actcctgttg ctgaccacca aagttcagtg catcgtcttt gttcagcccc aggttacggc
     6061 gtttacgttc actacctgcc cgtgccgcag cggcggaatc gtggaatggc gggttacaca
     6121 aggtcgcatc gtattgctcg tttttatgga tgatgccgtt aaaaatcgcc ccactctctt
     6181 tttgccgacg cagacgaatg gcgcggttaa gccccggatt agcactgatg atcgcctgcg
     6241 cactgcttaa cgcctggctg ctggtttcac taccagtaaa acgccagcca tattcatgta
     6301 cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg ctggcattag
     6361 ctgaaattga tcctgttgct tctgccagta aatcggcaag gtgatgaata taatccgccc
     6421 gacccggtac tggtgggcag agaaaaccgt ccgggatatc ccagttcgct acggcgtaaa
     6481 aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca aagtctacgc
     6541 tttgctcccc ggcgggtgta agcgtgagga attgcctgag ttcaggattg acctgacaaa
     6601 gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc ggtttctggg
     6661 cggacatagc attctccttt aagcagcggc gtaagatacc cgcataacgc gacagggtaa
     6721 atatctggct gcgtttatca tttctataaa ccttcacatg gctatggttt atggcaattc
     6781 atcataaaaa tcagcattat gcatatcatt gatttgtctt gttttgtaaa aacatatcgt
     6841 tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt ttcatcgcgc aggcgaccta
     6901 cacttatctg gcactacccg ttgtatttat gaggatgtca ttatgaaaaa gtgcctcaca
     6961 ctactgattg ccaccgtcct gagcggaatc tctctcacgg ctgatgccgc gcaaccgatg
     7021 agtaacctgg acagtggtca actacggccc gccggtaccg tttcggcaac tggcgcatca
     7081 aacctaagcg atctggagga taaactggca gaaaaagcgc gcgaacaagg cgcgaaaggt
     7141 tatgtcatta attccgcagg cggaaatgac cagatgttcg gtactgcaat catctacaaa
     7201 taaccccacc aacgtaactg cattcgcctc tggatgcagt tttcgtccga tttgtaagca
     7261 aaaaacatca aatatgaacg caatgtaaat aaatgtattt ctttttcgcg caatgggtga
     7321 tagaaaatcg cgccaagtga taatgcttat caaaattatt atcacttgca cggacgctat
     7381 cacgggatta acagtggcat cgcatccgca gagatgcttt ctcgtgacag tgaaaatttc
     7441 accatataag aaaaagtcac ctgcaaaatg gaaaacaatc gcaatttccc tgccagacaa
     7501 tttcattcgc tcacgttctt tgccggtctt tgtattggta ttacgcctgt ggcacaggca
     7561 ctcgctgccg aagggcaagc taacgcggat gacacgctgg ttgtcgaagc atcaacgcct
     7621 tcgctttatg cgccacaaca atctgccgat ccgaaattct cgcgtccggt agcggatact
     7681 acccgcacga tgacggtgat ttctgaacaa gtgattaaag atcagggtgc aaccaacctt
     7741 accgacgcgc tcaaaaacgt ccccggcgcg ggtgcgtttt ttgcgggtga gaacggtaac
     7801 tccaccactg gcgacgccat ttatatgcgc ggtgccgata cctctaacag tatttatatc
     7861 gatggcattc gcgatatcgg cagcgtctcg cgcgatacct tcaataccga gcaggtcgaa
     7921 gtaattaaag ggccgtccgg caccgactac gggcgcagcg cgccgacagg ctcgatcaat
     7981 atgatcagca aacagccgcg caatgattcc ggcattgacg cctccgccag tattggcagc
     8041 gcctggttcc gccgcggcac gctggacgtc aatcaggtca ttggtgatac cactgcggtg
     8101 cgcctgaatg taatgggcga aaaaacgcac gatgccggac gcgacaaagt caaaaatgag
     8161 cgttacggcg tcgccccttc tgtcgctttt ggccttggta cagcgaatcg tttgtatctt
     8221 aattatctgc atgtcaccca acacaacacg ccagacggcg gcattccgac catcggttta
     8281 ccgggttatt ctgccccatc tgcgggaaca gcagccctga atcattccgg aaaagttgat
     8341 actcataact tttacggcac agattccgat tacgacgatt cgatcaccga caccgctacc
     8401 atgcgttttg agcacgacat caacgataac gccaccattc gcaacactac gcgttggtcg
     8461 cgcgtaaagc aggattacct gatgacggcg attatgggtg gagcgtcgaa tattacccag
     8521 cccaccagcg atgtgaatag ctggacgtgg tcacgcacgg cgaataccaa agatgtgagt
     8581 aacaaaattc tcaccaacca gaccaacctg acctcgacgt tctataccgg cgctatcggt
     8641 catgatgtca gtaccggcgt ggaatttacc cgcgaaacac aaactaacta tggcgttaat
     8701 ccggtgacgt taccggcggt aaatatttat catcctgaca gcagcattca tcccggcggc
     8761 ctgacgcgca acggcgcaaa cgccaatggt cagacggata ccttcgcaat ttatgccttc
     8821 gatacgctgc aaatcacccg tgattttgag ctgaacggcg ggatccgtct ggataattat
     8881 catactgaat atgacagtgc taccgcctgc ggcggcagcg ggcgcggtgc catcacctgc
     8941 cctgctggcg tggcaaaagg ttctccggtc accaccgtcg acaccgccaa gtcgggcaat
     9001 ctggtgaact ggaaagccgg ggcgctgtat cacctgacgg aaaacggcaa tatctatatt
     9061 aactatgccg tttcccagca gccgccgggc ggcaacaact tcgcccttgc gcagtctggc
     9121 agcggtaaca gtgccaaccg caccgatttt aaaccgcaaa aagccaacac cagcgagatt
     9181 ggcaccaaat ggcaggttct ggataaacgc ctgctactta ccgccgcgct gttccgtact
     9241 gatattgaaa atgaagttga gcaaaatgat gacgggactt attcgcaata tggtaagaaa
     9301 cgtgtcgaag gctacgagat atccgtggcc ggaaatatca cccccgcatg gcagatgatt
     9361 ggcggctata cccagcaaaa agcaactatc aaaaacggca aagatgttgc ccaggatggt
     9421 tcctcatcgc tgccgtatac cccggaacac gccttcacct tatggagcca gtatcaggca
     9481 accgatgata tttctgttgg tgcgggcgca cggtatatcg gcagtatgca taaaggttca
     9541 gacggcgcgg tggggacgcc agcatttacc gaaggttatt gggtcgccga cgccaaactg
     9601 gggtatcggg ttaatcgcaa tctcgacttc cagctaaacg tctacaacct gtttgatacc
     9661 gattacgtcg cctcaatcaa taagagcggc taccgttatc acccgggcga gccaagaacc
     9721 ttcttgctca cagctaatat gcatttctga ttcagatgtg gggcgcaagg ctcactaatg
     9781 ttgatggcga gagcaggact gcctgatgcg ctgcgcttat caggcctaca aggttgtgca
     9841 atacactgaa ttcacatcat ttggtaggcc ggataaggcg ttcacgccgc atccggcacc
     9901 tgagcaccat tgcctgctgt taaaaatcgg tgtggggcgc aggccccact ttttggagaa
     9961 cttgtatgat gtaccacatt cccggcgtgt tatcgccaca ggacgtcgcc cgttttcgcg
    10021 aacacctgga acaagccgaa tgggtggatg gacgcgtcac caccggcgca caaggtgcgc
    10081 aagttaagaa caatcaacaa gtagacaccc gcagcgccct gtacgccgcc ctgcaaaatg
    10141 aggtgctgaa cgcggttaac caacatgctt tgttctttgc cgcggccttg ccgcgtaccc
    10201 tttccacgcc gctgtttaat cgctatcaga acaatgaaac ctatggtttt catgtggatg
    10261 gcgcagtacg cagccatcca caaaacggct ggatgcgcac tgacctttct gccacgctgt
    10321 ttttaagcga tccagaaagc tacgacggcg gcgaactggt cgttaatgac accttcgggc
    10381 aacatcgggt aaaactcccg gcaggcgatc tggtgttgta tccctccagc agcctgcatt
    10441 gcgtgacacc cgtaacccgc ggcgtacgag tggcatcatt tatgtggatc cagtcgatga
    10501 tccgtgatga taaaaagcgc gccatgctgt ttgaactgga caacaatatt cagtcgctga
    10561 aaagccgcta cggcgaaagt gaagagatcc tgtcactgct caatctttat cataatctgc
    10621 tgcgggaatg gtcggagatc tgacgcttga actgcccgag ttcgtcccta tattagctac
    10681 acttaactgt acaagtattg atatggggag gacgatatgg catccggttg ggctaatgat
    10741 gacgccgtca acgaacagat caacagtaca attgaagatg cgattgcccg cgctcggggt
    10801 gaaattccgc gtggcgaaag cctgtatgaa tgtgaagagt gcggtgcccc tatcccgcag
    10861 gcccgtcggg aagccattcc tggcgtgcgc ttatgcattc attgtcagca ggataaagat
    10921 ttacaaaaac cagcttatac aggatataat cgcagaggtt cgaaagacag ccagttacgt
    10981 taactacaac tggcgggatg tatagcgagt ataatttgta ttttgcgtgg cattccgtgc
    11041 ctttaacgct accgtgactg ccacgcggta taaaacaagt ccataagtac aacaaataaa
    11101 tggtttatca gtatgttaga tatcaatcaa tttatttgaa caaggcggtc aattctcttc
    11161 gattttatct ctcgtaaaaa aacgtgatac tcatcacatc aacgaaacaa cgtcacttat
    11221 acaaaaatca cctgcgagag attaattatg aaaactatca atactgttgt tgctgctatg
    11281 gctctttcaa ctttgtcatt tggcgtgttc gcggcggaac cggtaacggc atcccaggca
    11341 cagaacatga ataaaatcgg cgtggtttct gccgatggcg catccaccct cgatgccctg
    11401 gaagcgaaac tggctgagaa agccgcggcg gccggtgcca gtggatacag catcacttct
    11461 gccaccaaca acaataaatt aagcggtact gcggtaattt ataagtaatt cgtaggacgg
    11521 ataaggcgtt tacgccgcat ccggcaacag atgcctgatg cgacgctacc gcgtcttatc
    11581 agacctacaa aatccgaacc gttggctgga tcaggcgttt acgctacgtt cagcacaagt
    11641 atttccctgt ctgtttgccg acagacgcat atgctctaac cctcattgat cctatgttac
    11701 ccttgtttgc ccgtccgcca ctggacgggc tttttttagc tggctaactg ctgacagaaa
    11761 gcctgcaacg tctcttccgg cataccaatc tgccgcgctg catcacaaat ggcctgccag
    11821 cttcccgcat ccagtggaat cccctgctcc tgccgttcgc gacgcgtgtt cacttcccac
    11881 tcgcccggta gcaaaatcgg cttatcgtca tcatgcggcg aggctttcac ccactcggca
    11941 aaggcttcgg tctgtgcact acaatccggc gcgccgaaca gttccgggtt gataatgata
    12001 gtggtcatgc agttgagaat ggcatcggga ctggtttgta acgtttcctg atgcgtcgtt
    12061 ttaccgccag aaagcgcccc gccaagaatt tcacacatcg ctgcaagggc gtagccttta
    12121 tgttcggcaa aggtcaacag cgaacccaac ggcgactcct gcattaccgc cggattggtc
    12181 gtcggcacgc cgttaacgtc aatcaggcaa cctggcagca cggggacgcc tttatgccag
    12241 gcgacgcggg ttttgccaaa tgcaatggcg ctggtggcgt aatcgagcaa cagcgggaaa
    12301 ttatctttac gagggaaaac cacacagaac ggattggtgc caaagcggct gtcgcgaccg
    12361 tggaacggcg cgaccattgg aataccgacc acgctaacaa agtggataga gacaaacccc
    12421 gccgctgcac actgctccgc ccagtaaccg atacggccga tatgatgcga gttatgtagc
    12481 gccacggcgg caatgccgtg ctgatgcgct ttctcaatcc ccagcgccat cgcttcgtgt
    12541 gccgcgacct gaccaaatgc gcgatcgcca tcgagcgtga ccgccgcccc cgcctcttta
    12601 acgattttgg catgatgatt aatttgcaga tgcccctgac tccaggaacg cacatagctt
    12661 gggatcatgc caataccatg tgaatcatgc cctgccaggt ttgccgcgat taaatgatcg
    12721 gcaactaatt tcgcttcctg ttcctcgcta cccatctgac gaaatacagc ctgaataaaa
    12781 ctgtgcagcg tctgagcatc aaagcgatga ccactttcca tggttaactc ctgtttatgt
    12841 tatgtgtttg ttgttttttt atgctgcacg ccgggcgcgg gaaggtcaat aaagatgcag
    12901 gaatttcata ttgcggtggg gtaaaagccg gatgacatgg ctcatccggc tgagataaaa
    12961 attaaaatgc ctgattaatg cgcgccaggc cgtcgttgat agttgccgct tcacccgtag
    13021 ccaccaggca gcaagccatc tggattttca gcgattcagg aatcggttcg ctgccagcaa
    13081 ggcaacgctc aatccactgc gccgtggttt ccggatcttt tgcttgtggc agtaactcgc
    13141 taccagcagt gtcctgtttt tcatacagta cccgcatccc ttcacggtca atgagattga
    13201 tctgcgggca gcgctgcgga ttagcataca cttcaccttc agtaccatgc ataagtagcg
    13261 cccgcccgcc gatatcacta aagaacttcg cgacgcgtcc aatgtattcc ggatgcgaaa
    13321 cgctggaaag acgcagtgcc tcaccttcgg caaatggcgt cgccagtttc gccagggtat
    13381 gtgcactgtt acgcacgccc atccgccagc gcatcgccag ttgtttttcc agcggcgggc
    13441 aaaacgcgcc gacaggcata aagacaggtt gatgttcgtc gagcttcgcc tgcgcctgcc
    13501 cgccgtgcaa cgttggcgta atacccatca gttcgaaaat ggtttcagtc agcacgcggg
    13561 ttggatcttc gctaaccccg tgaaccacca caggaaaacc gagtttgtgc agcagaatcg
    13621 ccagcaacgg cgtcaggttg gcctgtttac gcgcgccgtt gtaactgggg atgacaatcg
    13681 gcatcggctt gcctgcaggc ggcgtcagct tgatggtgtg attttgcatg gcttcgtaaa
    13741 agccgagcat ctctgcttcc ccttcccctt tgatacgtag cgcaatcaat acgccgccca
    13801 actcaaggtc agggacttca ccattgagca tatgggcata cagaccgcgc gcagtatccc
    13861 ggtctaaatc gcgcgcgtgg tttttcccgc gcccgatctc tttaatgatt ttgcgatagt
    13921 ccatttacga ctccttacct gactcacatc attaacgccg ccgacggcgt ggggatttcg
    13981 ttttttcttt ctttttaact ataccttcag ggacttccgg ttgctctatc ggaaataccg
    14041 gtaatgcatc cagtagtcgc ttgccataat ttttggtcag caagcgtttg tcgtagataa
    14101 ccacttcccc ccagcaaccg tggcttcgaa tcagtcgccc aacctgctga attaggttaa
    14161 acgaagcgct cggcaggctt tgcacctcaa acggatagcg gttgaggctt ttcagccatt
    14221 caccttcggt gatcaccacc ggactgtcga tgggcggaaa agcgatcttg tggatatgca
    14281 cctggctgag cagatcgcct ttcagatcaa gaccttcggc aaaggactgt aagcccacca
    14341 gcacgctacg ctcaccgttg gcaacgcgtt tgcggtgcag ttcaactaag cgataacgtg
    14401 gctgatcgcc ctgcaccagc aacatcaggc gtaaatccgt cacatagtcg agaaagcgct
    14461 gcatcgcacg cccgctggca aacagcacca acataccgag atgttttttg ctctccacct
    14521 gttcacggaa aaaggccgcc atttcggcaa tgtgctgctc ttcgttgtcg atggaaggct
    14581 caacgcgcat ccggggaata acaattttgc cctgttcaca gtggttaaaa ggggaatcca
    14641 gtgccacaaa acggtcgccc gctttctctt tcagcccact catctcctgc aaacgcgaaa
    14701 aactgttcag cgaacgcaag gttgcggagg taacaataat gtgcggaata ctgcgccaca
    14761 gcagcctttc cagctgatcg ctgacacgta ttcccacgca gtgaaaccaa aggtgtagct
    14821 gcccttcgcg ctcttcccgc gtcgcccatt tggtcaccgg cgcaccggaa gattgcgcca
    14881 gagacgccag ccgccagagt ttgctttgcg cctcgaacat ccccaacgcg cggttcatct
    14941 gcaaaatcaa ccgatgcagg cgtacaatgt catggctgcc ggttttctca cttaaatcgt
    15001 taagaaataa ctccgccaga ccacgcagca tctcggtgag ttttgccagc cgctggcaga
    15061 tctccagcac ttcatcaggc agttcgccca tcgcaaaacg gtgctctgcc tcctgcccgg
    15121 caggcatgta gagattgaga atgttgttta acgaggcgat aagctcatac agctcttcac
    15181 aatgcgcatt caaacgttca gggatcgcca gcggtgggat ggtcttcggg cgaaactgct
    15241 ccatgcaggt ggcgaccagt ttggtgaaca ggtccagttg gagccggtac cagggcgcag
    15301 tgatttcggc gctcatctcc agcgcatccc gcgccacatc cggcaggtga tgaccttcgt
    15361 ccagcaccag cagtaaattt ttcgggtcag gcaataccgc ttcgctttcc atcgccgcca
    15421 tcaccagcgc atggtttgcc accaccactt ccgcttcctg aatttcccga cgagcgacaa
    15481 aaaacggaca ttcgcgatag taataacagt tgcggttaag gcaactggcc ttgtcggtac
    15541 tcaaacgacg ccagagatca tcatctatag cgatatcagt atgatcgcgc agaccatccc
    15601 atttataagt gtcgagatcg cccttcagct tcgcacaacg tttttgctct tcctgattgt
    15661 tcggcgtcag ttcgtcgtca agaaacgcca gcagatcctg ttgcgtgggt tcagtgctgg
    15721 cgagcgctgt cagattacgc gggcaaacgt agcgcccacg cccaaaagcg gcagtgaatt
    15781 taagatcggg aatgatcttt ttcagcagcg gtaaatcttt gctgtagatc tgatcctgca
    15841 atgccacgtt ggcggtactt accaccagcg ttttttgctc ttcgcgggca atggcgatgc
    15901 cggggatcaa ataggagagc gttttcccaa cgccggtagg ggcttcaatc gccagatgcc
    15961 gcccttcttc tccggccagc gttttggcga cgtccgcaat catctgccgc tgcggcgcac
    16021 ggggaataaa gtcggggatc tgttcctgaa gcgccttata ccaggcggca atttgcgctt
    16081 taagcgcggc ggttaatgcc atgagaaaac ctgaaatact gtataaacag ccaatattgt
    16141 ggcatttttc ggttttcagg ggtaatggtt tttctgtttc tggaaagcgg ctcgcaataa
    16201 ccagcgggat gatgcgtaaa gcgcttcgcg ctaaattcga acaacatgca gaactgcgta
    16261 cactcttgct ggcaaccgcg tccgcaaaac tggttgagca tacgcaaaat gatgcttact
    16321 ggggagatgg cggtaatggt cagggcaaga atcacctggg gtacctttta atggcgttgc
    16381 gcggacaatt ggctgcggag aagtaacgca tcgggtggct tgtgggcgtt aaaacagaga
    16441 aatctttttg ccgcgctcgc aaatggctga caagtgcttg ttgtgaaatg ccggatgcgg
    16501 cgcgagcgcc ttatccggcc tacaaaaaca tgcacattca atatattgca gagttcacgt
    16561 aggcctgata agcgtagcgc atcaggcagt tttgtgtttg tcatcagtct catgccgggc
    16621 atagcccggc ataaaagatt actacgcagc ggcaggttta cgcggacggc ggcgacgttg
    16681 ttgttcgcct gccggtttgg catcgccgag acgacgagac ggtttttctg cgggcttcgc
    16741 gttaccagac ttcgcaccac tttccgcacg gcgtggttgc tgttgaccgc gaccaccgcc
    16801 ctgtccacga ccgccgccac gttgctggcg accgttctgg atcggttcgg ctttgattga
    16861 cgggtccggc tcataccccg gaatcgcaat gcgcgggatc tcttttttca gcagtttttc
    16921 gatatcacgc agcagtttgt gttcatcaac acacaccagc gacaatgctt cgccagttgc
    16981 cgccgcacgg ccagtacgcc cgatacggtg aacataatct tccggtacgt ttggtaactc
    17041 atagttgacg acgtgcggca actcttcgat atccaggccg cgcgcagcga tgtcagttgc
    17101 caccagtaca cgaatatcgc ccgatttaaa atcggccagc gcacgagtac gcgcaccttg
    17161 cgatttatta ccgtggatcg ccgcactacg gatgccatct ttattaagct gttcagccag
    17221 atggttagcg ccatgtttgg tacgggtaaa caccagcacc tgctgccagt tccctttccc
    17281 aatcatatgc gacagcaatt cgcgtttgcg tttcttatcg acaaagtgaa cgtgctgggt
    17341 cacctgatca gacgcagtat tgcggcgtgc cacttcgatt tccagcgggt tgtgcaacag
    17401 tttttccgcc agggctttaa tatcgtcaga gaaggtcgcg gagaacaaca ggttctggcg
    17461 cttcgctggc agttttgtta acacgcgacg gatatcgtgg ataaagccca tgtcgagcat
    17521 gcggtcagct tcatcaagga cgaggatttc aacctgatcc agcttcactg cattttgatg
    17581 ttccaggtcc agcagacgtc ccggcgttgc caccagcaca tcaacgccgc cgcgcaactt
    17641 catcatttgt gggttaatgc tgacaccgcc aaacaccacc agcgaacgaa tgttcagata
    17701 tttgctgtaa tcacggacgt tttcgccaat ctgcgccgcc agttcacggg tcggggtaag
    17761 aatgagcgcg cgtaccggac gacgcccttt ggcgtgcggc tggcgagtga tcaggtgttg
    17821 caacaacggc agcgtaaagc ccgctgtttt gccggtaccg gtctgggcgc tggccatcag
    17881 gtcgcggcct tccagcaccg cagggatcgc ctgctgctga ataggggtgg gttcacggta
    17941 accctgctcg gcaacggcgc gcaggatatc agggcttaaa cccaaagaat cgaaagacat
    18001 aactactccg aaccgccccg accgttaccg gtgtagtttt cagggagata ctgaaaagga
    18061 aaatgacaaa aaccacaatg tcatgaggga gcggagtgta gcagtttttg tgacgcagcg
    18121 cataaattat ccctgtgact ggcgtagcta aaattcttag ccatactgga caactcccct
    18181 atggagtcat aatcttaatc aatcatttga ttaagaatgg actgtgcgat gaataatcct
    18241 gccatgacaa tcaagggtga acaggcgaaa aaacagctga ttgctgcggc actggcacag
    18301 tttggtgaat atggaatgaa cgccaccacc cgcgagatag ccgcccaggc cgggcagaat
    18361 atcgcagcca tcacctacta cttcggttcg aaagaagatt tgtatctcgc ctgcgcccag
    18421 tggattgccg attttattgg cgagcagttc cgtccgcacg ctgaagaagc cgaacgcttg
    18481 ttcgcacaac cacagccgga tcgggctgcc atccgcgaac tgatccttcg cgcctgcaag
    18541 aacatgatta agctgctcac ccaggatgac accgtcaacc tcagcaaatt tatctcccgt
    18601 gagcagctct ctcccacggc agcctatcac ctggtgcacg aacaggtaat tagtccgcta
    18661 cacagccacc ttacacgcct gattgccgcc tggaccggct gcgacgccaa tgacacccgc
    18721 atgatccttc atacccatgc gttgattggt gagattctgg catttcgtct tggcaaagaa
    18781 accattttgt tacgtaccgg ctggaccgcg ttcgatgaag aaaagaccga actgatcaac
    18841 cagacggtga cctgtcatat tgacctgatt ttgcaaggat tatcgcaaag gagtttgtag
    18901 tgatgaaaaa acctgtcgtg atcggactgg cggtagtagt acttgccgcc gtggttgccg
    18961 gaggctactg gtggtatcaa agccgccagg ataacggcct gacgctgtat ggcaacgtgg
    19021 atattcgtac ggtgaatctt agtttccgtg ttggggggcg cgttgagtcg ctggcggtgg
    19081 acgaaggtga tgcgatcaaa gcgggccagg tgctgggcga actggatcac aagccgtatg
    19141 agattgccct gatgcaggcg aaagcgggcg tttcggtggc tcaggcgcag tatgacctga
    19201 tgcttgccgg gtatcgagat gaagaaatcg ctcaggccgc cgcagcggtg aaacaggcgc
    19261 aagctgccta tgactatgcg cagaacttct ataaccgcca gcaaggactg tggaaaagcc
    19321 gcaccatttc ggcaaatgac ctggaaaatg cccgctcctc gcgcgaccag gcgcaggcca
    19381 cgctgaaatc agcgcaggat aaattgcgtc agtaccgttc cggtaaccgc gaacaggaca
    19441 tcgcccaggc gaaagccagc ctcgaacaag cgcaggcgca actggcgcag gcggagctga
    19501 atttgcagga ttcgacgctg gtcgctccgt ctgatggcac gctgttaacg cgcgcggtag
    19561 agccaggcac ggtgcttaat gaaggtggca cggtatttac cgtctcgctg acgcgtccgg
    19621 tgtgggtacg cgcctatgtt gatgaacgta atcttgatca ggcccagccg gggcgcaaag
    19681 tgctgcttta taccgatggt cgtccgaaca aaccgtatca cgggcagatt ggtttcgttt
    19741 cgccgactgc tgaatttacc ccgaaaaccg tcgaaacacc ggatctgcgt accgacctcg
    19801 tctatcgcct gcgtattgtg gtgaccgacg ccgatgatgc gttacgccaa ggaatgccgg
    19861 taacggtgca attcggtgac gaggcaggac atgaatgatg ccgttatcac gctgaacggc
    19921 ctggaaaaac gctttccggg catggacaag cccgccgtcg caccgctcga ctgcaccatt
    19981 cacgccggtt atgtgacggg gctggttggg ccggacggtg cgggcaaaac cacgctgatg
    20041 cggatgctgg cgggattact gaaacccgac agcggcagtg ccacggtgat tggctttgat
    20101 ccgatcaaaa atgacgctgc gctgcacgct gtgctcggct atatgccgca gaaattcggc
    20161 ctgtatgaag atctgacggt gatggaaaat ctcaatctgt acgcggattt gcgcagcgtc
    20221 accggtgaag cacggaagca gacttttgct cgcctgctgg agtttacatc tcttggcccg
    20281 tttaccggac gcctggcggg caagctctcc ggtgggatga aacaaaaact cggcctggcc
    20341 tgtaccctgg taggcgaacc gaaagtgttg ctgctcgatg aacccggcgt cggcgttgac
    20401 cctatctcac ggcgcgaact gtggcagatg gtgcatgagc tggcgggcga agggatgtta
    20461 atcctctgga gtacctcgta tctcgacgaa gccgagcagt gccgtgacgt gttactgatg
    20521 aacgaaggcg agctgctgta tcagggagaa ccgacggccc tgactcaaac catggccgga
    20581 cgcagctttc tgatgaccag cccgcacgag ggcaaccgca aactgttgca acgggcattg
    20641 aaactgccgc aggtcagcga cggcatgatt caggggaaat cggtacgtct gatcctcaaa
    20701 aaagagacca cgccagacga tattcgccat gccgacggga tgccggaaat cgacatcaac
    20761 gaaactacgc cgcgttttga agatgcgttt attgatttgc tgggcggtgc cggaacctcg
    20821 gaatcgccgc tgggcgcaat attacatacg gtagaaggca cacccggcga gacggtgatc
    20881 gaagcgaagg aactaaccaa gaaatttggt gattttgccg ccaccgatca cgtcaacttt
    20941 gccgttaaac gcggggagat ttttggtttg ctcggaccaa acggcgcggg taaatcgacc
    21001 acctttaaga tgatgtgcgg tttgctggta ccgacctctg gtcaggcgct ggtgctgggc
    21061 atggatctga aagagagttc tggtaaagcg cgccagcatc tcggctatat ggcgcaaaaa
    21121 ttttcgctct acggcaacct gacggtcgaa cagaatttac gctttttctc tggtgtgtat
    21181 ggcttacgcg gtcgggcgca gaacgaaaaa atctcccgta tgagcgaggc gttctgcctg
    21241 aaaagtatcg cctctcacgc gaccgatgaa ctgccattag gttttaaaca gcggctggcg
    21301 ctggcctgtt cgctgatgca tgaaccggac attctgtttc tcgacgaacc gacgtccggc
    21361 gttgatcccc tcacccgccg tgaattttgg ctacatatca acagtatggt agagaaaggc
    21421 gtcacggtga tggtcaccac ccactttatg gatgaagcgg aatattgcga ccgcatcggc
    21481 ctggtgtacc gcgggaaatt aatcgccagc ggcacaccgg acgatttgaa agcgcagtcg
    21541 gccaacgatg agcaacccga tcccacgatg gagcaagcct ttattcagtt gatccacgac
    21601 tgggataagg agcatagcaa tgagtaatcc gatcctgtcc tggcgtcgcg tgcgggcgct
    21661 gtgcgtcaaa gagacgcggc agattgttcg cgatccgagt agctggctga ttgcggtagt
    21721 gatcccactg ctactgctgt ttatttttgg atacggcatt aacctcgact ccagcaagct
    21781 gcgcgtcggg attttactgg aacagcgtag cgaagcggcg ctggatttca cccacaccat
    21841 gaccggttcg ccctacatcg acgccaccat cagcgataac cgtcaggaac tgatcgccaa
    21901 aatgcaggcg gggaaaattc gcggtctggt ggttattccg gtggatttcg ccgaacagat
    21961 ggagcgcgcc aacgccaccg caccgattca ggtgatcacc gacggcagtg agccgaatac
    22021 cgctaacttt gtgcaggggt atgtcgaagg gatctggcag atctggcaaa tgcagcgcgc
    22081 ggaggataac gggcagactt ttgaaccgct tattgatgta caaacccgct actggtttaa
    22141 ccccgcggcg ataagccagc actttattat ccccggtgcg gtgaccatta tcatgacggt
    22201 catcggcgcg attctcacct cgctggtggt agcgcgagaa tgggaacgcg gcaccatgga
    22261 ggctttgctc tctacggaga ttacccgcac ggagctgctg ctgtgtaagc tgatccctta
    22321 ttactttctc gggatgctgg cgatgttgct gtgtatgctg gtgtcggtgt ttattctcgg
    22381 cgtgccgtat cgcgggtcgc tgctgattct gttttttatc tccagcctgt ttttactcag
    22441 taccctgggg atggggctgc tgatttccac gattacccgc aaccagttca acgccgctca
    22501 ggtggccctg aacgcggctt ttctgccgtc gattatgctt tccggcttta tatttcagat
    22561 agacagtatg cccgcggtga tccgcgcggt gacgtacatt attcccgcac gttatttcgt
    22621 cagcaccctg caaagcctgt tcctcgccgg gaatattcca gtggtgctga tagtgaacgt
    22681 gctgtttttg atcgcttcgg cggtgatgtt tatcggcctg acgtggctga aaaccaaacg
    22741 tcggctggat tagggagaag agcatgtttc atcgcttatg gacgttaatc cgcaaagagt
    22801 tgcagtcgct gctgcgcgaa ccgcaaaccc gcgcaattct gattttaccc gtgctgattc
    22861 aggtgatcct gttcccgttc gccgccacgc tggaagtgac caacgccacc atcgccatct
    22921 acgatgaaga taacggcgag cactcggtag aacttactca acgttttgcc cgcgccagcg
    22981 cctttaccca tgtgctgctg ctgaaaagcc cgcaggagat ccgcccgacc atcgacacac
    23041 aaaaggcgtt actactggtg cgtttcccgg ctgacttctc gcgcaaactg gataccttcc
    23101 agaccgcgcc gttgcagttg atcctcgacg ggcgtaactc caacagtgcg caaattgccg
    23161 ccaactacct gcaacagatc gtcaaaaatt atcagcagga gctgctggaa ggaaaaccga
    23221 aacccaacaa cagcgagctg gtggtacgca actggtataa cccgaatctc gactacaaat
    23281 ggtttgtggt gccgtcgctg atcgccatga tcaccactat cggcgtaatg atcgtcactt
    23341 cactttccgt cgcccgcgaa cgtgaacaag gtacgctcga tcagttgctg gtttcgcccc
    23401 tcaccacctg gcagatattc atcggcaaag ccgtaccggc attaattgtc gccaccttcc
    23461 aggccaccat tgtgctggcg attggtatct gggcgtatca aatccccttc gccggatcgc
    23521 tggcgctgtt ctactttacg atggtgattt acggtttatc gctggtggga ttcggtctgt
    23581 tgatttcatc gctctgttca acgcaacagc aggcatttat cggcgtattt gtctttatga
    23641 tgcccgccat tctcctttcc ggttacgttt cgccggtgga aaacatgcca gtgtggctgc
    23701 aaaatctgac gtggattaac cctattcgcc actttacgga cattaccaag cagatttatt
    23761 tgaaggatgc gagtctggat attgtgtgga atagtttgtg gccgctactg gtgataacgg
    23821 ccacgacagg gtcagcggcg tacgcgatgt ttagacgtaa ggtgatgtaa cttcttatct
    23881 ttcgccagca aagacaccac cgcagggccg gcaaggattg ccagccctgc aattgccagc
    23941 aaaaagttgt tttccagtac gcgggacaac agccacagca caatcagcgc tgcaccaaaa
    24001 taccaggtcg tggtcagctc ttccagcacg tctgccacct cacgccaggg ttgctcgggg
    24061 tggcgctgaa acaccagcat catcactacc gtgacacaat acagtgcgca taatcctacg
    24121 ccgacgtgaa ccagagtttt actcatccag cccaccacca gcagcgccac taccagtaaa
    24181 tgcaacataa tctgccagca acttaggcca gttgcgacac gaacacgttg ttgccacttc
    24241 atggcttctc tcctgaagaa tttatctcta cttattcaga gtaccgaact ttacggaaaa
    24301 ttccgtaaga ccgcaccttt ttgtgacgcc gactacacta tttcaatgag ataagaggca
    24361 aagacaggaa aattatgccc gatcaaacac aacaattttc gttcaaggtg ctcaccatca
    24421 atattcacaa aggctttacc gcgtttaacc gacgcttcat tttgccggaa cttcgcgacg
    24481 ccgtgcgtac cgtcagcgcc gatattgttt gcctgcagga agtgatgggc gcgcacgaag
    24541 ttcatccgct gcatgtggaa aactggcccg atacctcgca ctacgagttt ctcgccgaca
    24601 ccatgtggag cgatttcgcc tacggtcgca atgccgtata cccggaagga catcacggta
    24661 atgcggttct ttcacgttat cccattgaac attatgagaa tcgtgatgtt tcggtcgatg
    24721 gtgcggaaaa gcgcggcgtg ctctactgcc gcattgtgcc gccgatgacc gggaaagcga
    24781 ttcatgtgat gtgcgtacat ctgggcctgc gtgaggcgca ccgtcaggcg cagcttgcga
    24841 tgctcgccga atgggtgaat gagctaccgg acggcgaacc ggtattggta gcgggtgatt
    24901 tcaacgactg gcggcaaaaa gctaatcatc cgttaaaagt gcaggccggg ctggatgaga
    24961 tttttacccg cgcccacgga cgcccggcgc gcacgtttcc ggtgcaattt cctctactac
    25021 gactggacag gatctacgtc aaaaatgcca gcgccagcgc gccaaccgcg ttgccgctgc
    25081 ggacatggcg acacctttct gatcatgccc ctttaagtgc ggagattcat ttatgaaatg
    25141 tagctggcgc gaaggcaata agatccagtt gctggaaaac ggcgagcaat attatcccgc
    25201 ggtgtttaag gcgattggag aggcacaaga acgcatcatt cttgaaacgt ttatctggtt
    25261 tgaggatgac gtcggcaaac agctgcatgc ggcgctattg gcagcagcgc aacgcggggt
    25321 aaaagcggaa gtcttgctgg atggctacgg ttcgccggat ctcagcgatg agtttgtcaa
    25381 tgaactgacg gcagctggcg tggtgttccg ctactacgat ccccgccctc gcctttttgg
    25441 tatgcgcacc aatgtgtttc gccggatgca tcgcaaaatt gtggtgatcg acgcgcgtat
    25501 cgcctttatt ggcgggctga attactccgc cgagcatatg tccagctacg gcccggaggc
    25561 caaacaggat tacgcggttc gcctcgaagg gccgattgtc gaagatattc tccagtttga
    25621 gctggaaaac ctgccaggac agagcgcggc gcgacgctgg tggcgacgtc atcataaagc
    25681 ggaagagaac cgccagccgg gagaagcgca ggtattgctg gtctggcgcg ataacgaaga
    25741 acatcgcgat gatatcgaac gtcactatct gaaaatgctc actcaggcgc ggcgggaagt
    25801 gattatcgcc aacgcctact tcttccccgg ctatcgtttt ttacacgcct tgcgtaaagc
    25861 ggcacggcgc ggggtgcgga tcaaactgat cattcagggc gaaccggata tgccgattgt
    25921 cagagtcggc gcacgtttgc tgtataacta tctggttaaa ggcggcgttc aggtgtttga
    25981 gtaccgccgc cgcccgctcc acggcaaagt ggcattgatg gacgatcact gggcgacagt
    26041 agggtccagt aatctcgatc cgctcagttt gtcactgaat ctcgaagcaa atgtcatcat
    26101 ccacgatcgt aattttaacc agacgctgcg cgataatctg aacggcatta tcgccgcaga
    26161 ttgtcagcag gtcgatgaga ccatgctgcc gaaacgcacc tggtggaacc tgaccaaaag
    26221 cgtgctggcg ttccactttt tacgccactt cccggcgctg gtgggctggc ttccggcgca
    26281 cacgccacgt ctggcgcagg ttggtccccc cgcacaaccg acaatggaaa cgcaggatcg
    26341 ggtagaaact gaaaacacgg gggtaaaacc ctgatgagta aatcacaccc gcgctggcgc
    26401 ttagcaaaga aactcctcac ctggctgttt tttatcgcgg tgattgtgtt gctggtggtc
    26461 tacgccaaaa aagtggactg ggaagaggtc tggaaggtca tccgcgacta caatcgcgtt
    26521 gcgctgctta gtgcggtcgg gctggtggtc gtcagttatc tgatttacgg ttgctacgac
    26581 ctgctcgccc gtttctactg cggtcacaaa ctggtgaagc gccaggtgat gctggtgtcg
    26641 tttatctgct acgccttcaa cctgacactc agtacctggg tcggcggcat tggtatgcgc
    26701 tatcgtttgt actcccgact ggggttaccg ggcagcacta ttacgcggat tttttcgctc
    26761 agtattacca ccaactggct aggctatatt ttgctagcgg gggttatctt taccgcaggc
    26821 gtggtggagt tgccggatca ctggtatgtc gatcaaacta cgctgcgtat tctcggtatt
    26881 ggcttactga tgattatcgc ggtttatttg tggttttgcg ctttcgcgaa gcaccgccat
    26941 atgaccatca aagggcaaaa gctggtgctg ccttcgtgga aattcgccct cgcccagatg
    27001 ctgatttcca gcgttaactg gatggtaatg ggagcgatta tctggctgtt acttggtcaa
    27061 agcgtgaact atttctttgt actgggcgtg ttgctggtta gtagtattgc tggcgtcatc
    27121 gtgcatattc cggcgggaat tggtgtgctg gaagcggtgt ttatcgcgct actggctggg
    27181 gagcatacct ccaaaggcac aattatcgcc gccctgctcg cttaccgtgt gctgtattac
    27241 tttatcccac tgctgctggc gctgatctgt tacctgttgc tggaaagcca ggcgaagaag
    27301 ctacgggcga aaaatgaagc ggcgatgtga agtatagtta agttctttag tatgtgcatt
    27361 tacggctaat gaaaaaaccg cgtatgcctt tgccagacaa gcgttatagc tatagcggca
    27421 agaatcccaa agaagccgat aaaatagtaa tagagtgtta atgcacctat aaaagcacaa
    27481 cgaaccgaac tccataattc ttcactatgg ctttttcctg tcagttcaag gagcttattt
    27541 cgtccatgcg agataatgcc tgaccataag acaatcatta agcaacttat tacccaaatg
    27601 ggtcgttcac tcattaatgt ggcattcacg atgattacca gggccaaccc tgtcaacgtc
    27661 atcataatga taaaacggtg agaaccagga tcaaatttaa acaatagacc tgccagcata
    27721 ctgattaaaa acatcgatcc cgcagtgctg aacacgacga taatggcaga tacaggcgct
    27781 aaactcgcaa cgacaatcgc caccaaagag attaaaaaca aatatattac gcccgcgatg
    27841 gcggtattta tcttgtt
//
LOCUS       DADSZD010000048        27670 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.048, whole genome shotgun sequence.
ACCESSION   DADSZD010000048 DADSZD010000000
VERSION     DADSZD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.048"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..689)
                     /locus_tag="J1Z28_001828"
     CDS             complement(<1..689)
                     /locus_tag="J1Z28_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBA7716667.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLNWPDNLPALLQNIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPAHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPACEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(700..1701)
                     /gene="ltaE"
                     /locus_tag="J1Z28_001829"
     CDS             complement(700..1701)
                     /gene="ltaE"
                     /locus_tag="J1Z28_001829"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBA7716668.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1738..3459)
                     /gene="poxB"
                     /locus_tag="J1Z28_001830"
     CDS             complement(1738..3459)
                     /gene="poxB"
                     /locus_tag="J1Z28_001830"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBA7716669.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEEELRKLAQLLRYSSNIALMCGSGCAGAHK
                     ELVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQ
                     FPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTERKFLD
                     KALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYL
                     KMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQM
                     KLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGIKGIRVEKASEI
                     DEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIEL
                     AKTNWLR"
     gene            complement(3592..4560)
                     /gene="hcr"
                     /locus_tag="J1Z28_001831"
     CDS             complement(3592..4560)
                     /gene="hcr"
                     /locus_tag="J1Z28_001831"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBA7716670.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVSTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4572..6224)
                     /gene="hcp"
                     /locus_tag="J1Z28_001832"
                     /gene_synonym="priS"
     CDS             complement(4572..6224)
                     /gene="hcp"
                     /locus_tag="J1Z28_001832"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBA7716671.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6368..7267)
                     /gene="lysO"
                     /locus_tag="J1Z28_001833"
     CDS             complement(6368..7267)
                     /gene="lysO"
                     /locus_tag="J1Z28_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBA7716672.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNFAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNSMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7588..8283)
                     /gene="aqpZ"
                     /locus_tag="J1Z28_001834"
     CDS             complement(7588..8283)
                     /gene="aqpZ"
                     /locus_tag="J1Z28_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBA7716673.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8709..10367
                     /locus_tag="J1Z28_001835"
     CDS             8709..10367
                     /locus_tag="J1Z28_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBA7716674.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVLNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENIPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10364..11320)
                     /locus_tag="J1Z28_001836"
     CDS             complement(10364..11320)
                     /locus_tag="J1Z28_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HBA7716675.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            11471..12586
                     /gene="macA"
                     /locus_tag="J1Z28_001837"
     CDS             11471..12586
                     /gene="macA"
                     /locus_tag="J1Z28_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBA7716676.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLESGDEVVIGEAKLGAAQ"
     gene            12583..14529
                     /gene="macB"
                     /locus_tag="J1Z28_001838"
     CDS             12583..14529
                     /gene="macB"
                     /locus_tag="J1Z28_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBA7716677.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNSPAVEKVNATGGTEPVVNTASGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSGGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTVT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14602..14826)
                     /gene="cspD"
                     /locus_tag="J1Z28_001839"
     CDS             complement(14602..14826)
                     /gene="cspD"
                     /locus_tag="J1Z28_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBA7716678.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15149..15469
                     /gene="clpS"
                     /locus_tag="J1Z28_001840"
     CDS             15149..15469
                     /gene="clpS"
                     /locus_tag="J1Z28_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBA7716679.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15500..17776
                     /gene="clpA"
                     /locus_tag="J1Z28_001841"
     CDS             15500..17776
                     /gene="clpA"
                     /locus_tag="J1Z28_001841"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBA7716680.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     repeat_region   17989..18977
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17989..18016
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            19150..20133
                     /gene="cas1f"
                     /locus_tag="J1Z28_001842"
     CDS             19150..20133
                     /gene="cas1f"
                     /locus_tag="J1Z28_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1"
                     /protein_id="HBA7716681.1"
                     /translation="MSSNYLTPSDLKTILHSKRANIYYLEKCRVQVNGGRVEYVTSEG
                     KESYYWNIPIANTTALMLGMGTSVTQAAMREFAHAGVMVGFCGTDGTPLYSANEVDVD
                     VSWLSPQSEYRPTEYLQQWVSFWFVEDKRLAAAKRFQLIRLTHIDKYWSSSKMLREHA
                     FQPDVNALHTLLNRTCEEIDAAENHTQLMLVEAKLTKALYKMVSQTVGYGDFTRAKRG
                     GGIDMANRFLDQGNYLAYGLAAVAAWVTGIPHGLAVMHGKTRRGGLVFDLADLIKDAL
                     VMPQAFIAAMAGEDAQEFRQRCINIFQQADALDVMITSLQETAQALAKADQ"
     gene            20130..23363
                     /gene="cas3f"
                     /locus_tag="J1Z28_001843"
     CDS             20130..23363
                     /gene="cas3f"
                     /locus_tag="J1Z28_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3"
                     /protein_id="HBA7716682.1"
                     /translation="MNVLIISRCTKNARVESCRIIDQFAERTGDAAWQTVITLEGVNT
                     LRRLLRKTARRNTAVACHWLKKNGQTELMWIVGNIRRFNAQGRVPTNRTTQISLVNKE
                     ENRWQCAESIALLAAIAGLFHDFGKSGRSFQQSLTQKNTRSYQPYRHEWISLRLFQAF
                     VGDRPDDVWLAKLGELTADDESAILERLQKDTPQFSNSPFSSLQPLAKTVGWLILSHH
                     RMPENLSPQSEQSCKGCESWLEKQLNADWNAVNHRRDDWSQQDFAHVWQFPSGTPLQS
                     ASWREKARQIARRASNALGLKTYGQMEQLFPLHMARFCLMLADHYYSSSDAQQKWQDP
                     DYIVWANSDRQSGTLKQRVDEHLVGVAHHAYLLGRQLMHTRASLPAIARHKGFSQRAT
                     DVNYRWQDKAWDVARALRERSREEGFFGVNMASTGCGKTFANARIMYALADEREGCRF
                     TVALGLRTLTLQTGDALRSRLGLGEDELAVLIGSAAVNQLWQQEKIDNDCGSASQESP
                     AEEQQFVKYEGSLHTGALEKWLKDDSKLKELVSAPILVSTIDHLISATEGVRGGKQLP
                     AMLRLLTSDLVLDEPDDFDIADLHAVCRLMNWAGMLGTRVLLSSATLPPGLIQALFAA
                     YLAGRKMWQASCGINGRPVNICCAWFDEKDADATQIYDGPGFRDAHAKFVARRAVMLA
                     EKERLHFGRVASISSASSAIQDVTESVAQTVHTQMLKLHQAHRQRHESGKTVSLGLVR
                     FANINPLVAVTKALIAIPSPEDVCIHYCVYHSRHPLAVRSDIEKRLDRAFTRHNELDF
                     WNNEDIADALHNRPESHHLFVVLGTSVIEVGRDWDADWGIIEPSSMRSIIQFAGRIQR
                     HRRNVPTSENLVILRSNIKSLQKKAPAFCKPGFETKEHLLNSHDICELIPIADYRTIN
                     ALPRILSANNANKLAELEHSRLSAELMSSKSKHVVAAQWWRAPLSWNGWMQRQTPFRY
                     SPLPEAVFFLHMDDEESEARFYSRTGDNERKAQGNFRREEVRCASGVACWGVMDYQHV
                     LLNLADKQSCEIANVGEKFAEVRVPVSEEDAVDDWRYHPWLGVFRNM"
     gene            23693..25000
                     /gene="csy1"
                     /locus_tag="J1Z28_001844"
     CDS             23693..25000
                     /gene="csy1"
                     /locus_tag="J1Z28_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HBA7716683.1"
                     /translation="MEKTALIQFITDYIASRRQPKIDAFEKEAAKRLEQGEDASVIAL
                     ERQELEARYLPRNWLTDAAKRAGQIKLVTHGAKFSHGDSKASSFYLETSANESYLNTA
                     SLANVATDAIGNAAALDVAKLLQTDVNGDSLLASLKRGDYQALSTFAEDKVQLELWVA
                     GFSQAWTTGRPSSHKLAKQIYFPVADGYHLLCPLFSSSLAQAMYEKLTAVRFSEESKA
                     IRDARKAGKWHSQPDIWFPNLAEMHFGGTKPQNISLLNSVRGGRVWLLPSMPPTWATL
                     DRAPQNMRSIFSLRGDFNRAASGIVAQMTYLLKVDINNVHIRTVRAKYIDELIDLLFM
                     QASAFQQEKWQGWSAQSPDLPRHQQLWLDPWRSLSDETFKQEREKNDWQVTVADDFAR
                     WLNYRLKKSSFDVGAVEQKEWRSQSLFAQRMREMEAVLQEALK"
     gene            24997..25920
                     /gene="csy2"
                     /locus_tag="J1Z28_001845"
     CDS             24997..25920
                     /gene="csy2"
                     /locus_tag="J1Z28_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HBA7716684.1"
                     /translation="MSYLLLLPHIRIENANAVSGLTWGFPSMTHFLGYVHALSRKVVD
                     EFGVSFDGCAVVSHEQHIQAYSSGRDFQFALTRNPLTREGKTASFNEEGRMHLTVSLL
                     IECNGEITNGEYGRKVLCDYLKMLCQSHKLAGGSIVSMRDPQLFHAPEDEKQLRKIVW
                     RLMPGYALYDRSEWLAEHHQQHPDISLLDAWLDFAAIKYQAESPAEDNSAKWVYQPKP
                     MPGFLVPLMCGYQRISPVYAPGEVENARDTVTPFAFAEAVYGIGEWRGLHRTTDLQAL
                     MWRYRTTDTGYYCSATPVVDDFTFNEYDDLE"
     gene            25931..26932
                     /gene="csy3"
                     /locus_tag="J1Z28_001846"
     CDS             25931..26932
                     /gene="csy3"
                     /locus_tag="J1Z28_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HBA7716685.1"
                     /translation="MAKAPVAIKTASVLAFERKLATSDAVMYAGNWQGNEWQPIEIQE
                     KAVRGTISNRLKNAIVSDPTKLDAEIQKANLQRVDVASLPVDTDTLKVVFTLRVLGNL
                     STPSVCNDMAYQEALSGVIEGYISEHGFKELALRYALNLANGRFLWRNRIGAEQIQVK
                     VTANETSWTFNSHDFSLRQFDQGQAAVTQLAAIIEQGLSAKEWVMLTVEAQVRLGAGQ
                     EVFPSQELVLDSNSSKSRVLYQVAGIAGIHSQKIGNALRTIDTWHPKVDELGAIAVEP
                     YGSVTSRGVACRQPKEKLDFYTLLDNWVTKGMKPDVKQQHYVMAVLIRGGVFGEKSE"
     gene            26943..27497
                     /gene="cas6f"
                     /locus_tag="J1Z28_001847"
     CDS             26943..27497
                     /gene="cas6f"
                     /locus_tag="J1Z28_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HBA7716686.1"
                     /translation="MDHYLEIRVLPDPEFSSEMLMAALFAKLHRVLGARGQGDIGVSF
                     PDVNVMPGARLRLHGSAQALQALEASTWRKGLTDYCQCSPVTPVPEIKGWRVVSRVQV
                     KSNSQRLLRRSVKKGWLTEEQAIERLATQAEQRTDLPFLNMKSLSSQQLFKLFIRHGD
                     LLKEPVKGEFSSYGLSATATIPWF"
BASE COUNT         6901 a         6789 c         7426 g         6554 t
ORIGIN      
        1 cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt gctgataact gagcacttct
       61 ggcccagcgg cttcgaagat gcgatgttcg caggccggat gatctaacag cgccaccaga
      121 tagtgcagca ggttttccag cgcgatgggc gtggtgcgtg aacgtaccca gcgtggtggc
      181 gttaacacgg gcaagttgta aaccatatcg cgcatgactt cgaacgccgc tgaacctgca
      241 ccaacgataa ttccggcacg caattcggtc actgggacac ccgcttcacg aagaatatcc
      301 gccgtagcct gacgggcgcg cagatggtca gactgctcat gtgccggggc ctgcaacgaa
      361 ctgaggaaga ttaattgctt aacagggact tcgcgtagcg catcgcggac gttgagagcc
      421 acctggcgct cctgagcgat aaaatcaccg ccttctccca tgctgtgcac cagaaaatag
      481 acagtatcaa tattctgcaa cagggccgga aggttatccg gccagttgag atcgacttta
      541 tggcaactga cgtttgccag ttgcagtttt gcaagcctgt cgacatgacg tgccgccgcc
      601 aggatctgat gcccttgctg gctgagcgtg cgcaccagat gctgaccaat gtagccactg
      661 gcaccgagaa ccaaaatgcg ttgcggcacg tctttctcct taacgcgcca gaaacgcacg
      721 ccagtgggcg gcgacttctg ccagttgttc acgcgagacg tcaagatgcg tcaccaggcg
      781 gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc
      841 agcattttct tccccgacgc gaacaaacag catattggtg tcctgacgca tcacatccgc
      901 gcctgcttca cgcaactgct ccgccatcca ggcggcattg tcgtggtctt cctgcaaacg
      961 tgcgacgtta tttttcagag catacatccc ggcggcagcc agaatgccgg actggcgcat
     1021 cccgccacct gtcattttcc gccagcgaat ggcacgttta atgtaatcac gattaccgac
     1081 gagtaatgaa ccgaccggcg tcccaagacc tttcgacagg caaatggtga acgagtcaca
     1141 atattgtgtg atctctttca gttcgcagcc gtaagccacc acggcattaa agatgcgcgc
     1201 accgtcaaca tgcagcgcca gattgcgctt gcgggtaaat tcccatgcct cttttaagta
     1261 ttcacgcggc agcactttgc cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc
     1321 gaaatggata tcgtcgggtt tgattttcat cgccacttta tccagcggta gcgtgccgtc
     1381 ggcagctgcg tcgatgggtt gcggctgaat actgcccagc actgctgcgc caccggcttc
     1441 aaacagatag ttatgcgcgg cctgaccgac gatatactct tcgccgcgtt cgcagtgact
     1501 gagcagagcg accaggttgg cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc
     1561 agaaagctct gctgcatagt cctgcagagc attaacggta gggtcgtctc cgtaaacgtc
     1621 gtccccaacc ggggcggcca tcatcgcttc gagcatggcg cggctcggtc gggtaacggt
     1681 atcactgcgt aaatcaatca tggcatgtcc ttattatgac gggaaatgcc acccttttta
     1741 ccttagccag ttcgttttcg ccagttcgat cacttcatca ccgcgcccgc tgatgattgc
     1801 gcgcagcata tacaggctga aacctttggc ctgttcgagt ttgatctgcg gtggaatggc
     1861 taattcttct ttggcgacaa ccacatccac caacaccgga ccgtcgatgg agaaggcgcg
     1921 ttgcagagct tcatcgattt cagacgcttt ttctacacga atacccttaa tgccgcaggc
     1981 ttcggcaatt cgggcaaagt tggtgtcgtg taattcggtg ccgtcagtca aatagccacc
     2041 agctttcatc tccatcgcca caaagcccag cacgctgtta ttaaagacga taattttcac
     2101 tggcagcttc atctgcacta ccgagaggaa atcgcccatc aacatgctaa aaccgccatc
     2161 gccgcacatg gcgaccacct ggcgttctgg ctcggttgcc tgcgcgccca gcgcctgcgg
     2221 catggcgtta gccatcgaac catggttaaa cgaacctaac aggcgacgct tgccgttcat
     2281 ttttagataa cgtgccgccc acaccgttgg cgtaccaacg tcacaggtga aaatagcgtc
     2341 atcggcggca aaatgactaa tttgctgcgc cagatattgc gggtgaatgg ctttctcgct
     2401 cggtttagct aaatcgtcca gccctttgcg ggcgtcgcgg taatcttcca gcgccttatc
     2461 cagaaacttg cgttcggttt tttcttccac cagtggcagc aatgcgcgca gggttgattt
     2521 gatatcgccg accagcgcca tatctacctt gctatgcgcg ccgatgctgg ctgggttgat
     2581 atcaatctga ataattttgg catcggtcgg gtagaacgca cggtagggaa actgagtacc
     2641 gagcagcact aacgtatcgg cgttcatcat ggtatggaaa cctgacgaaa agccgattaa
     2701 tcccgtcatt ccaacatcat atggattatc gtattcgaca tgttctttac cgcgcagggc
     2761 atgaactata ggcgctttaa ttttcccggc aaactcaact aactctttat gcgcccccgc
     2821 acagccgctg ccacacatca gggcgatatt gctggaataa cgcagcagtt gcgccagttt
     2881 gcgtaactct tcttcttctt ccggtgttac taccggctgt ggcgcatgat accagtgggt
     2941 agttgcccct tctggcgcag gttttaacgc cacgtcgccc ggtaacacaa caacggaaac
     3001 gccacggtta agcaccgctt tgcgcatagc aattgccagc acttgtggga tctgctctgg
     3061 gctggaaaca agctcgcaat agtgactaca ttcgcggaat agctcttgtg ggtgggtttc
     3121 ctggaaatag ccgctgccaa tttcgctgga gggaatatga gcggcaatcg ccagtaccgg
     3181 aacgtgattg cggtggcaat cgaacaggcc gttgattaag tgcaggttgc cgggaccgca
     3241 cgatccggca cagaccgcca gttctccgct aagttgtgct tcagcgccag cggcaaacgc
     3301 cgccacttct tcatgccggg tggacatcca ctcgatggtg cccatgcgat taagactgtc
     3361 actaagaccg ttcagagagt cgcccgtgac tccccagatg cgtttcacgc ctgccgattc
     3421 gagtgttttg gcgatataag ctgcaaccgt ttgtttcatg gttctccatc tcctgaatgt
     3481 gatgacggta acaagtttag ttcatctgac ggagggggaa gggatgggag aaaaaggagg
     3541 cactaacggt taaatagccc gatgaaagga atatcatcgg gcatcaggcg attatgcgag
     3601 aaccaaatcc ccctgcggat ggcaggagca ggccagtacg taaccttcag cgatttcggc
     3661 gtcggtcagc gtcattgtgc tgctcaccgt atattcaccg gaaatcactt tcgtcttaca
     3721 gcagccgcaa acacccgcac gacaggcggc gacaaccgga acgttattgc tttccagcgc
     3781 ctccagtagc gtggtgctaa ccggagcgta aaattctcgt gccggttgca gtttggtgaa
     3841 tttcagaccg ctggtcgccg cttccgctac tggggtgaag aatttctctt taaagaaacg
     3901 cgtcacgccg agcgctttca cttcctgctc tacccaatcc atatatggcg cagggccaca
     3961 ggtcatcacg gtacgtgagg ctaaatcagg gacacctgcc agcagttcgc gagtgagacg
     4021 accagcgata aagccttcgg taacgttatt ttccgccacc agcgttaccg gatagttacg
     4081 ccactcatcg gcgaaaatca catcctgcgg cgtacgcacg ttgtagatca cctgcacatc
     4141 ggcctgtgga cggttcttcg ccagccagcg acgcatcgac ataatcggcg taacgccgca
     4201 gcctgccgcc agcaacagga atttatcttc tgctttatcg tcgcaggtaa attcccccat
     4261 cgcgtccgaa agccagagat aatcaccgcg ttttacatcg cgcgtcagcc accgggagcc
     4321 gacaccgtca tcaatccgcc gcacggtcag ggtgatgtat tcactcacgc ctggcgtgga
     4381 ggaaatggtg taagcacgca gcgtttccgc tgagttacgc acgctgacca gcgcatattg
     4441 cccggcgcga tacgggtagt aatcgtggca aatcagggaa atcgtccaca catccggcgt
     4501 ttcttgcgta atgtgatgaa cctgcatccg ccacgggcat tgattcgttg gcatcgtcat
     4561 cgacaaactc cttacgcgct caacagttgc ttcatgtctt cttcaacagt ggtgatagaa
     4621 cgcaggccga atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa accaggtgca
     4681 gtcgggccgg tgacgatatt tttcacaccc agagaaagca gcgtcagcag aatgacaatc
     4741 gctttctgtt caaaccagga gagcaccagc gacagcggca gatcgttcac cccacagccc
     4801 agtttctctg ccagcgttac cgccagaata attgctgagt aagcatcgtt acactgacca
     4861 gcatctacca gacgcggcag accttcgata tcgccaaact caagtttatt aaagcgatat
     4921 ttaccgcaag cgagggtcag gatcaggcag tcatccggca cgctggtggc gaaatcggtg
     4981 aagtagtgac gctcgccgcg tgcgccgtca cagccgccaa gcaggaagat atgacgcagt
     5041 ttttcacggc tcaccagatc aatcagcgta tcggctgcgc caagcagtgt ctggcgacca
     5101 aagcccacgg tgatcaggtg cggaatttcg ctgtacggga agcccgccat ctgttgcgcc
     5161 tgggcaatga ccgcagagaa atcttcgcca tccagatgac gcacacccgg ccagccaacg
     5221 atgctgcggg tccagatacg atcgtcataa gcgcctacgg ttgggtcgat gatgcagttc
     5281 gaggtcatca cgatggggcc tgggaaacga gcgaactcca cttgctgatt ctgccagccg
     5341 ctgccgtagt taccgaccag atgcttgaat ttacgcaact ccgggtaacc atgcgcaggc
     5401 agcatttcgc cgtgggtgta gacattaacg cccgtgcctt cggtctgttc cagcaggttg
     5461 tagagatctt tgagatcgtg accggaaatc agaatgcatt tacccgccgt cgctttgacg
     5521 ttgacctgag ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcgtc cagaatgctc
     5581 atcactttga agttcatctg gccgatttcc attgaacact caagaagcgc gttcatatcg
     5641 gcaggccagg tccccagcca cgccatgatt ttatggtact gggcataaat atcgttgtcg
     5701 tattgaccga gaacatgcgc gtgttccata taggccgccg cacctttcag gccatacagg
     5761 cacagcagac gcaggccgag aatgttttcg ccaatcgccg ctttatcttt gttaggggta
     5821 aattctgctg cctgacgttg cagctcgccg agatcgtcgc tcaccagttg caggtcagcc
     5881 atcgggttat cgacgcgggc gttggcatct acagccaggc attgtgcttt cagcgcctcg
     5941 cgcagggcaa tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa gttaacgttg
     6001 gtcagggttg agaaaaaggc acgtggcgca aagctgtcta catcgtggtt gatgatgccg
     6061 tattcacgtg ctttcactgc ccaggccgaa agcccttgca gcgccgcgat gagtaaatcc
     6121 tgaaggtcag aggtttccgc cgttttacca cacatcccct gcgcgtatga gcagccgttt
     6181 cctgccggag tacggatagt ttgttcacat tgcacacaaa acatgatcac accttttaaa
     6241 gttatattta atatacatgt ttaaggttaa gacgcttaac gcggggataa aagagatttt
     6301 taatgcaact ttaagggaga ttgatttagc gcaattttgg cggcagagat ctaccgccag
     6361 agaggtatta tgcagagaaa aaggcgatga gaatcggcac taacagacta agaataaaac
     6421 cgtgaacaat cgccgccggg accatatcca gcccgcctgt acgttgaaga acgggcaggg
     6481 tgaaatccat cgacgtcgca ccgcataagc ccagtgcagt agagcggcta cggcgaatca
     6541 gcccagggat tagcataata gcaatcagct cacgggccag atcattaaaa aacgccgcgc
     6601 tcccgattac cggaccaaaa gattcggtca ataaaatacc ggaaagagaa taccagccga
     6661 aaccggaggc cattgccagc gcggtattga tgggaagatc aagaataaag gcgttaatta
     6721 aaccaccaat taatgaactg gcaaccacca ccacggcgac aatcattccc cggcgattaa
     6781 ggacaatctg ctttaaggtc atgctattat tgcgcaactg aataccgacg aggaaaagta
     6841 gcagaattaa ggtgtattca ctggcttcgg tcgcgtgttg taagaaagcc agtccactca
     6901 gaccaatggc aaaaccaatc actactacgc cgcacagttt tagcgactcc agcgccatcg
     6961 caatacgcga cgggagtttt tcttgatgat ggtggttgcg ccatggcagg ccgcgctcca
     7021 gccacatcag ggcggcaaaa ttacacagta aaataacggt aatactgacg gcagaataat
     7081 gcagaattgc caacaagtta ctggcgaggt tatcgagaaa cgccagactg atacccataa
     7141 aaaagagaat aaggtaaacc atccaactta atagctgatt aataactctt aatgcagctt
     7201 tttggcgaag cggaatgagg taacccacaa tcaggggaac cagaatgatt aacagcccag
     7261 aaaacatgaa aaaccagtcc ttgcaaagaa gattcgaaat gcgcgatgac acactactga
     7321 aagcggaagg acgagtaaag ttgcaattaa aaggaaatgt tatgcatcag agggggattt
     7381 ttaattctat atgagtgttt agcgttgttg cgctcctttc atgtgcaatg ccatataaag
     7441 ccactggcgc agggagcggc cagtcgccgc cgctccctgc ccccccggcc ttcggggaac
     7501 gcctcggcga ttttaacgcc accaacaccc gtgcggtttg taacgtcgaa gataaaacca
     7561 ccagggcaga caacatgcca atgctaatta atcgcgtttt tccagtagag tccggtaaat
     7621 cagaccaccg ataataccgc cgacaattgg caccacccag aagaaccaca gttgttctaa
     7681 tgcccagccg ccctggaaga tagcaaccgc agtactgcgc gccgggttaa cggaagtgtt
     7741 agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatgg caattggcgc
     7801 aaaaccagca ggagcgaatt tgtcagttgc gccgtggatc accagcagga aacctgcact
     7861 caataccagt tcaactacca gcgcggaaag catggaataa ccgcctggtg aatgctcgcc
     7921 ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa cccgttttac cactggcaat
     7981 taaatacagc agcgccgccg caacaatacc gccgacaacc tgggcaatta cgtagccaac
     8041 gacttctttt gccgggaaac gtccgccagc ccataaacca atagtgaccg ccgggttaaa
     8101 atgaccacca gaaatatgac caacagcaaa ggccattgtc agaacggtca gaccgaacgc
     8161 caacgccacg ccggcaaaac caatgcctaa ttccgggaag cctgcggcca gtacggcact
     8221 accacagccg ccaaaaacaa gccagaaagt accaaaacat tcagctgcta attttctgaa
     8281 catatccacc tcaattaaaa attgaccctg tgaaaaatat ggtcgtttta tagggtcgtc
     8341 gtaaaaaagt gacgacggaa atgatgcgcg gctattttaa aaacgaaggc aagtcattca
     8401 ccagataaat aaatccagta aatttgattt aaggcaacag cgggttgccc catatagtca
     8461 tttgtctgat tgacagtgta gtgcaagcga aagatttaat cctttaggcg taataaaaaa
     8521 taatttatca tgctaattat ttgattttgt tgtttttgca gacttgtcag caagagggag
     8581 tataacgcga ttattcgctc atttttcaga catttgccat gcttaaatgt gatgtcatca
     8641 cgtattagca tggcctttcc cgttatactg ccagcgtaaa ggataagtca catatttctg
     8701 gaggggatat gattcttgag cgcgttgaaa ttgtgggttt tcgcggtatc aaccgtttgt
     8761 cgttgatgct ggaacaaaac aacgtcctga ttggggagaa cgcgtggggt aaatccagct
     8821 tgctggacgc cttaaccctg ctgctatcgc cagaatcaga tctctaccat tttgagcgcg
     8881 acgatttctg gttcccgccg ggagatatca acgggcgaga acatcatctg catattattt
     8941 tgaccttccg cgaatcgctg ccaggccgac atcgggttcg ccgctatcgg ccgctggaag
     9001 cgtgctggac gccatgcacc gatggttatc accgtatttt ttatcgtctg gaaggggaga
     9061 gtgcggaaga cggcagcgtg atgacactcc gcagttttct cgataaagac ggacatccga
     9121 ttgatgttga ggatattaac gatcaggctc gtcatctggt gcgtttaatg ccggtgctgc
     9181 gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg cacggtgcta aatgtcccca
     9241 atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg tgaattatcc tcacatccgc
     9301 aaaatctctc tgatgggcag attcgtcagg gactttccgc aatggtgcaa ctgcttgagc
     9361 attatttttc tgagcaggga gctggacagg cgcgatatcg tttaatgcgg cggcgagcca
     9421 gcaatgagca acgaagctgg cgctatctgg atatcatcaa ccgaatgatt gaccgacctg
     9481 gagggcgctc gtatcgggtt attttgctcg gcctgtttgc tactttattg caggcaaaag
     9541 gcacattgcg actggataaa gacgcccgtc cattgttgct gatcgaagat ccagaaaccc
     9601 gtttacaccc cattatgctt tcagttgcct ggcatctgtt gaaccttctg ccattgcagc
     9661 gcatcgctac taccaactcg ggagagttgc tttcgttaac gccggtagag catgtttgcc
     9721 gactggtacg tgagtcctct cgcgttgccg cctggcgtct gggaccgagt ggcttgagta
     9781 ccgaagatag ccgacgcata tcctttcaca ttcgttttaa ccgtccgtca tcgctgtttg
     9841 ctcgctgctg gctgttggtg gaaggggaaa cggaaacctg ggttatcaac gaactggcgc
     9901 gccagtgtgg acaccatttc gatgccgaag ggatcaaggt cattgagttt gcccagtccg
     9961 ggctaaagcc gctggttaaa tttgcccggc gaatggggat tgaatggcat gtactggtcg
    10021 atggcgatga agcagggaag aaatatgccg ctacggtacg cagcctgttg aataacgatc
    10081 gggaagccga acgagaacat ttaacggcgt taccggcgct ggatatggaa cattttatgt
    10141 atcgccaggg attttccgat gtgttccacc gcgtggcgca aatcccggaa aatataccga
    10201 tgaatctgcg caaagttatc tcgaaagcga tccatcgctc ttccaaaccc gatcttgcca
    10261 ttgaagtggc aatggaggcc ggacgtcgtg gtgtggattc cgtaccgact ctgctgaaaa
    10321 aaatgttctc acgcgtgctg tggctggcgc gcggtcgggc cgactaacta caaaacatcg
    10381 tggccatttg tggctgaata gcgtcgagca tctcatagcg ccgacggtat tcagcccgtt
    10441 ttttactggc gatttcggca atctcttttc gtgctatctg tgctggaagg cggtaatggc
    10501 gttcagcatc acatacgccg ccaaccgatt cccagaaagc gttgtaatcg gcatggatct
    10561 tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg cgtttcattg ctgacggcaa
    10621 taatctgctc tacctgcaaa cgttgggcaa acagacaggc cgcttccatc acgaggcgtt
    10681 tgggaaatag cccgtggcag gctttcgtcg cattctggat ttcctggtgt ggaatttccc
    10741 attttgcgcc ttgcagtccg ccaataaaca tcgtcctttt cccctgatat tcacacaggg
    10801 taaaagtgag ctctgccaga ggaatacctt cgctgttgcg gaacaggatg gtgctgtcac
    10861 cttctttatc cattgagatc atcatggtca gctcaagcgt gaactgctcg ccgtttttgc
    10921 cttccagctt cgccagttgc agcccggggg tattcaaata taagctgaat tcttccgccg
    10981 acatacaccc gcggagtaac gcataatggt aacgtaacgc ctccagcaat tgcttacggc
    11041 taagattcgc cgcaaggtaa gggcgatgta gacgtacagg cagtcgcggc tggcgcgtta
    11101 acaacacatt gagattaggc cagtgggaaa gttcgttcat ccactcaacg cttaaacgcg
    11161 gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact acggcgatgc cagaatttac
    11221 ccggccgaca ctgtcctcgt gccagactaa gaaaaagtga caggctgctg agagattcag
    11281 atgacgtaaa ggtccgttca gttagctgcg acatattcat aaaatcaatg gttatacatg
    11341 acgtcgattt aaccattgcg tatcttaacc aaacatcaat agtgtgatta ttaacgtaaa
    11401 ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc ggattggcat atggagtatt
    11461 cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt tacgttattg cgatggtgat
    11521 agtcatcgcc gggctgatta cgttatggag aattcttaac gcacccgtgc cgacttatca
    11581 gacgctgatt gtgcgccccg gtgatttaca gcaaagcgtg ctggcgaccg gaaagctgga
    11641 cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt cagttgaaaa cgctgtcggt
    11701 ggcgattggc gataaagtaa aaaaagacca gcttttaggg gttattgatc ctgaacaggc
    11761 tgaaaaccag atcaaggagg tggaagcaac actgatggag ctacgcgcgc agcggcagca
    11821 ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc cgtcagcaac gtcttgcaca
    11881 aacgcaagcg gtttcacagc aggatctcga taacgccgcg acggagatgg ctgtgaaaca
    11941 ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat caggcctctc tcgatacggc
    12001 taaaaccaat ctcgattaca cccgcatcgt tgccccgatg gccggggaag tcacgcaaat
    12061 caccactctg caaggccaga cggtgattgc cgcacaacaa gcaccgaaca ttctgacgct
    12121 ggcagatatg agcaccatgc tggtaaaagc gcaggtttct gaagcggatg taatccacct
    12181 gaagccgggg caaaaagcct ggtttacggt gcttggcgat ccactgacgc gctacgaggg
    12241 gcaaatcaag gatgtgctac cgacgccgga aaagattaac gacgctattt tctattacgc
    12301 ccgttttgaa gtacccaacc ccaatggttt actgcggctg gatatgaccg cgcaagtgca
    12361 tattcagctc accgatgtga aaaatgtgct gacgatccct ctgtcggcgt taggcgatcc
    12421 ggttggcgat aatcgttata aagtcaaatt gttgcgtaat ggtgaaacac gcgagcgtga
    12481 agtgacgatt ggcgcacgta acgataccga tgttgagatt gtcaaagggc tggaatcggg
    12541 cgatgaagtg gtgattggtg aggccaaact aggagctgcg caatgacgcc cttgctcgaa
    12601 ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc aggttgaggt gctgaagggc
    12661 atcagcctcg atatttatgc cggtgagatg gtagcgattg tcggcgcttc gggttctggt
    12721 aaatcgaccc tgatgaatat tctcggctgc ctggataagg ccaccagcgg cacctatcgc
    12781 gtcgccggtc aggatgttgc cacgctggac gccgatgcgc tggcgcaact gcgccgcgag
    12841 catttcggct ttattttcca gcgttaccat ttgctttcgc atttaaccgc cgagcagaac
    12901 gttgaagtac ccgccgtcta tgccggtctt gagcggaaac agcggctgct tcgcgcccag
    12961 gagttactgc aacgactggg gctggaagac cgtacagagt attacccggc acagctttcg
    13021 ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga tgaacggcgg tcaggtaatt
    13081 cttgccgatg aaccgaccgg cgcactggac agccattctg gcgaagaggt gatggcgatc
    13141 ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg tcacccacga tccgcaggtt
    13201 gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg aaattgtgcg caattctccc
    13261 gccgttgaaa aagtgaatgc tactggcgga acggaacctg ttgtcaacac ggcgtctggc
    13321 tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga tggcatggcg ggcgctggca
    13381 gcgaataaaa tgcgtacttt actgaccatg ctggggatta ttatcggtat tgcgtcggtg
    13441 gtttccattg tcgtggtggg cgacgccgcc aaacaaatgg tgctggcgga tattcgttct
    13501 attggtacga atactattga tgtctatccc gggaaagatt ttggcgatga cgatccgcaa
    13561 tatcagcagg cgctgaagta cgacgactta atcgccatcc aaaaacaacc gtgggttgcc
    13621 tctgccacgc ccgccgtctc gcaaaacctg cgcctgcgtt ataacaatgt tgatgtggct
    13681 gccagtgcca atggcgtgag cggcgattat tttaatgtct atggcatgac cttcagtgga
    13741 gggaacacct ttaatcagga gcagctgaac ggtcgtgcgc aggtcgtggt tctcgacagt
    13801 aatactcgcc gccagctttt cccccataaa gcagatgtgg ttggcgaggt gattctggtc
    13861 ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa aacagtcgat gtttggtagc
    13921 agtaaagtgc tgcgtgtctg gctgccatac agcacgatgt ccgggcgagt tatggggcag
    13981 tcgtggctga actccattac tgtcagggtg aaagaaggct ttgacagcgc tgaggcggaa
    14041 cagcaactca cgcgtttact ttcactgcgc cacggtaaga aggatttctt tacctggaac
    14101 atggatggcg tcttgaaaac cgttgaaaag accacacgta ctttacaact gtttctgacg
    14161 ctggttgcgg tgatttcgct ggtggtgggc ggtattggtg taatgaatat tatgctggtg
    14221 tcagtgaccg agcggacgcg ggaaattggc attcgcatgg ctgtaggtgc gcgagcaagc
    14281 gatgttttgc aacagttttt gatcgaagcc gtactggttt gcctggttgg tggcgcgttg
    14341 ggaataacac tgtcactgtt aattgctttc accttgcagc ttttcttacc cggctgggag
    14401 attggttttt caccgttggc actgctgctg gcgtttctct gctcgacggt caccgggatt
    14461 ttatttggct ggttacccgc acgaaatgcg gcacgactgg atccagtaga tgctctggca
    14521 cgagagtaat ttttgagata aaaatgccag ccgatcgggc tggcattttg cctttaggat
    14581 gtacacaatg agacagaaga gctatgcgac tgccgcttct acttcgacgg gcacaataac
    14641 actggcgtga ttgccttttg gcccctggtg gacatcaaac tgaacggatt gtccagcttt
    14701 tagcgttctg taaccatcca tctgaatggt ggaataatga gcgaaaatat cttcgccgcc
    14761 gccttcaggg cagatgaaac caaacccttt ggcattgttg aaccacttaa cagtaccctt
    14821 ttccatgctt cgacatcctt cgcaaatctt atacaagtaa gatggaataa accggggtca
    14881 gagagggggc tgttcaaaac ctcgccaact ctagaaatac aatttagaga attagggcga
    14941 gccgtcaagc atttgacagg ggccaggggg caggtatgaa tcaaaaattt gaagcagtta
    15001 acgctattga caggaatgtg acagatgtcg ctgatgccaa cgatagatga tagttatcta
    15061 tcatgtggag tagattggtc aggcaaataa gctcttgtca gcggcagggc gttctgccga
    15121 taaccgtaac cgaagatgat aactgacaat gggtaaaacg aacgactggc tggactttga
    15181 tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat
    15241 attagtcaat gatgattata ctccgatgga gtttgttatt gacgtgttac aaaaattctt
    15301 ttcttatgat gtagaacgtg caacgcaatt gatgctcgct gttcactacc aggggaaggc
    15361 catttgcgga gtctttaccg ctgaggttgc agaaaccaaa gtggcaatgg tgaacaagta
    15421 cgcgagggag aatgagcatc cattgctgtg tacgctagaa aaagcctgaa tgcaggcata
    15481 aaaattgggg gaggtgccta tgctcaatca agaactggaa ctcagtttaa atatggcttt
    15541 cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc gagcacttgt tactggcgct
    15601 gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct
    15661 ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc gttctgcctg ccagtgaaga
    15721 ggagcgcgac acacagccga cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca
    15781 tgtccagtcc tccggtcgca atgaggttac cggtgcaaac gttctggtcg ctatctttag
    15841 cgaacaggag tcgcaggcgg catatctgtt gcgcaaacat gaagtcagcc gtcttgatgt
    15901 ggtgaacttt atctctcatg gcacgcgtaa agacgagccg acacagtctt ctgatcctgg
    15961 cagccagcca aacagcgaag aacaagctgg tggggaggaa cgtatggaga atttcacgac
    16021 gaacctgaat cagcttgcgc gagtgggcgg aatcgaccca ctgattggtc gtgagaagga
    16081 gctggagcgt gctattcagg ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg
    16141 ggagtctggt gtcggtaaaa ccgcgattgc ggaggggctt gcatggcgaa ttgttcaggg
    16201 cgatgtgccg gaagtgatgg ctgactgtac gatttactct ctcgatatcg gttctctgtt
    16261 agcgggcaca aaatatcgcg gcgactttga aaaacgtttt aaagcgttgc tcaagcagct
    16321 ggagcaggat actaacagca tcctgtttat tgatgagatc cacaccatta tcggtgcggg
    16381 tgcagcgtct ggtggccagg tcgatgcggc taacctgatt aaaccgttgc tctccagcgg
    16441 taaaattcgc gtaattggtt cgacaaccta tcaggagttc agcaacattt ttgagaaaga
    16501 ccgtgctctg gcgcgtcgct tccagaaaat tgatattact gaaccgtcaa tcgaagaaac
    16561 tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac
    16621 cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa tacattaacg atcgtcatct
    16681 gccggataaa gcgattgatg ttatcgacga agcgggcgct cgcgcacgtc tgatgccggt
    16741 aagcaaacgc aagaaaaccg ttaatgtggc ggatattgaa tccgtggtgg cccgtattgc
    16801 acgcattcca gagaagagtg tttctcagag tgaccgcgat accctgaaaa acctcggcga
    16861 tcgcctgaaa atgctggtct tcggtcagga taaagccatt gaggcgctga ctgaagccat
    16921 taagatggcg cgtgcaggtt taggtcacga acataaaccg gtcggttcgt tcctgtttgc
    16981 cggccctact ggggtcggga aaacagaggt gacggtacag ctttcgaaag cgttgggcat
    17041 tgagcttctg cgctttgata tgtccgagta tatggaacgc cataccgtca gccgtctgat
    17101 tggtgcgcct ccgggatacg ttggttttga tcagggcggt ttgctgactg atgcggtcat
    17161 caagcatcca catgcggtgc tgctgctgga cgaaatcgag aaagcgcacc cggacgtgtt
    17221 caacatcctc ttgcaggtga tggacaacgg tacactgacc gataacaacg gacgcaaagc
    17281 ggacttccgt aacgtggtgc tggtgatgac cactaacgcg ggggtacgtg aaaccgagcg
    17341 caaatccatt ggtcttatcc accaggataa cagcaccgat gcgatggaag agatcaagaa
    17401 gatctttaca ccggaattcc gtaaccgtct cgacaacatt atctggttcg atcatctgtc
    17461 aaccgacgtg atccatcagg tggtggataa attcatcgtc gagttgcagg ttcagttgga
    17521 tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt aactggctgg ccgagaaagg
    17581 ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa
    17641 accgctcgcc aacgaactgc tgtttggttc gctggtggac ggcggtcagg tcaccgtcgc
    17701 gctggataaa gagaaaaatg agctgactta cggattccag agtgcacaaa agcacaaggc
    17761 ggaagcagcg cattaatctg attgtctggt aggttggtta agtccgtgat ctcgtcaggg
    17821 gttacggact ttttatttat ggggggagga ggttcagacc ctttttttaa tgatgatgct
    17881 aagttattga taattagtgc tgcgggtagg taaggataaa aaagggtggc agcaggagat
    17941 tgagatggtt ttgctttatt aacaacgggc taaacgtgta gtatttgagt tcactgccgt
    18001 acaggcagct tagaaaaccg gggctatatt caccgaacgc cagcaaaagt tcactgccgt
    18061 acaggcagct tagaaaggaa atctaaaaaa atttattggc gatacaaggt tcactgccgt
    18121 acaggcagct tagaaacgaa gccgttgcgg atctttaaca gcgccacggt tcactgccgt
    18181 acaggcagct tagaaagctg taaattggct gaccgtcgca aaatgagggt tcactgccgt
    18241 acaggcagct tagaaagcgc gctgaattgc cagaatcgtg ggcgcacagt tcactgccgt
    18301 acaggcagct tagaaaagtt ttttacccca cacctccatt ttcacattgt tcactgccgt
    18361 acaggcagct tagaaaaggt cgcaacaaaa tccgggtcaa acgatccgtg ttcactgccg
    18421 tacaggcagc ttagaaagtg aatcacggcg cttattgctg gctgaaatgg ttcactgccg
    18481 tacaggcagc ttagaaaagc tcaacggtac gcgcctgctc gtcaatcgcg ttcactgccg
    18541 tacaggcagc ttagaaaaat acgcccgact ttttgtggca tttctgctgg ttcactgccg
    18601 tacaggcagc ttagaaatcg ccgagataac tgcgcacctc gcgatctgcg ttcactgccg
    18661 tacaggcagc ttagaaaggc gagcggtcgg ggatcccctt atataatttg ttcactgccg
    18721 tacaggcagc ttagaaaggc gcgcccacct cttcctccag ccccggggcg ttcactgccg
    18781 tacaggcagc ttagaaaggt acgctcggaa cacagcgggc agcactaacg ttcactgccg
    18841 tacaggcagc ttagaaaaac tggcgcgacg tccggggcaa acctggtggg ttcactgccg
    18901 tacaggcagc ttagaaactg aacgttgaag agtgcgaccg tctctccttg ttcactgccg
    18961 tacaggcagt attcacacta cgatagtgtt agacgtttgg tcgtgcaatg acactctcaa
    19021 cttcaaacca ttagcgttag cacgcaataa caatcgtaat aattgcgatg gaaatcaatt
    19081 ttcagcacat aaatcaatgc tgtactaagc ccaatacctt caaatataaa ataatcacag
    19141 gatgtgttta tgtcttcgaa ttaccttacg ccttccgatc tcaaaaccat tctccactcc
    19201 aaacgcgcca atatttatta tctggaaaaa tgccgggtac aggtgaatgg tgggcgggtg
    19261 gagtatgtta ccagcgaagg taaagagtcg tactactgga atatccccat tgcgaatacc
    19321 acggcgttga tgctgggaat gggaacttcc gttacccagg cggcgatgcg tgaatttgct
    19381 catgccgggg tgatggtagg cttttgtggt acggatggca cgccgctgta ttcagcaaat
    19441 gaagtggatg ttgatgtctc ctggctcagc ccacaaagtg aatatcgccc tacagagtat
    19501 ctccagcagt gggtttcttt ctggtttgtt gaagacaaac gacttgctgc cgcgaaacgt
    19561 tttcagttaa tccgcctgac acatatcgat aagtactggt ccagctcgaa aatgctgcgt
    19621 gaacacgcat ttcaacctga tgttaatgcg ctgcataccc tcctgaatcg tacttgtgaa
    19681 gaaatagacg cggcagaaaa ccatacgcag cttatgctcg ttgaggccaa attgactaaa
    19741 gcgttataca aaatggtcag tcaaaccgtg gggtatggcg atttcactcg cgcaaaacgg
    19801 ggcggtggta tcgatatggc caaccgcttc ctcgatcaag ggaattatct ggcttatgga
    19861 ctggcagcag tggcggcgtg ggtaacaggt atcccgcacg gtttagctgt aatgcatggt
    19921 aagacacgtc gcggcgggct tgtttttgac cttgccgatc tgattaaaga tgctcttgtt
    19981 atgccgcaag catttattgc cgcaatggcg ggagaagatg ctcaggagtt tcgtcaacgc
    20041 tgtattaata tctttcaaca agcggatgcg ctggatgtca tgatcacctc tctgcaggaa
    20101 acggcccagg cactggctaa ggctgaccaa tgaatgtcct aattatctca cgctgtacga
    20161 aaaatgctcg cgtggaaagc tgccgtatta ttgatcagtt tgccgaacgc actggtgacg
    20221 cagcatggca aacggtgatt actctggaag gcgttaatac actccgccgt ttactgcgca
    20281 aaaccgccag gcgaaatacc gcagtggctt gtcactggct gaagaagaat gggcaaacgg
    20341 agctgatgtg gattgtcggc aatattcgtc gctttaatgc tcaaggccgc gttcccacaa
    20401 atcgaacaac gcaaatcagc ctggtgaaca aagaagaaaa tcgctggcag tgcgcggaaa
    20461 gtattgcact gctggcggct atcgctgggc tatttcatga ttttggtaaa tcaggacgca
    20521 gctttcagca aagcctgact caaaagaata cccgcagtta tcaaccttac cgacatgaat
    20581 ggatctcact gcggctcttt caggcgtttg tcggtgatcg gcccgacgat gtgtggctgg
    20641 cgaagcttgg cgaactcacg gcagatgatg aatctgcgat tcttgagcgc ctgcaaaaag
    20701 acactcctca attttcaaat agcccgttca gttcgctgca accattagcc aaaacggtag
    20761 gctggctgat cctttcccat catcgaatgc cggagaatct ttctccacag tcggaacaat
    20821 catgtaaagg gtgtgaaagt tggctggaga agcaacttaa tgctgactgg aatgccgtta
    20881 accaccgcag agatgactgg tcacaacagg attttgcgca cgtctggcag tttccatcag
    20941 gaacgccgct tcaaagcgcg agctggcggg aaaaagcgcg tcagatagcc cgccgggcaa
    21001 gcaacgcgct cggtttaaag acatatgggc agatggaaca gttgttcccc ttacatatgg
    21061 cgcgtttttg tctgatgctt gccgatcatt actattcctc aagcgatgcg cagcaaaaat
    21121 ggcaggaccc ggattatatc gtctgggcaa atagcgatcg ccaaagcggc acgctcaaac
    21181 aacgggtaga cgaacacctt gtgggcgtcg cgcatcatgc gtatctgctt gggcggcaac
    21241 tgatgcacac acgggcatcg cttccggcaa ttgcccgaca taaaggtttt agccagcggg
    21301 ccacagatgt gaattatcgc tggcaagata aagcctggga tgtggcgcga gcgttacgtg
    21361 aacgaagtcg tgaagaggga tttttcggcg tcaatatggc ctcgacgggg tgtggtaaaa
    21421 cctttgcgaa cgcccgcatt atgtatgcac ttgccgacga gcgagaaggg tgccgcttta
    21481 ccgttgcgct gggattgcgc acgctgactt tacaaaccgg cgatgctctg cgcagtcggc
    21541 ttgggctggg tgaggatgag ctggcggtac tgattggttc ggcggcagtt aatcaactgt
    21601 ggcaacagga aaaaatagac aatgattgcg gaagtgcttc gcaagagtcg ccggcagagg
    21661 agcaacagtt cgttaagtac gaaggcagcc tgcataccgg ggcgctggaa aaatggttga
    21721 aggatgacag caaacttaag gagttagtaa gcgcgccgat tctggtctcg acgattgatc
    21781 atcttatttc cgcgaccgag ggcgtgcgag gcgggaaaca gcttccggcg atgttgcgcc
    21841 tgttaaccag cgatttagtg ctcgacgaac ctgatgactt tgatattgcc gatcttcatg
    21901 cggtatgtcg attgatgaac tgggccggga tgctgggaac gcgggtatta ctttcttccg
    21961 cgacgttacc gccaggtttg attcaggcgc tgttcgcggc ttatcttgct gggcgaaaaa
    22021 tgtggcaggc gtcttgtggt atcaacggca ggcctgtcaa tatatgctgc gcctggtttg
    22081 atgaaaaaga tgctgatgcc acgcaaattt acgacggacc tgggtttcgt gatgctcatg
    22141 ccaaatttgt tgcacgacga gcggtaatgc tggctgaaaa agaacgactg catttcgggc
    22201 gtgtagcttc aatttcatct gcatcaagcg cgatacaaga tgttaccgaa agtgtggccc
    22261 agacggtgca tacgcaaatg ctgaagttgc atcaggcgca tcgccagcga cacgaaagcg
    22321 gtaaaaccgt ttcgctgggc ctggtgcgct tcgcgaatat caatccactg gttgcggtga
    22381 ccaaagccct gattgccatt ccttcaccag aagatgtctg cattcattac tgtgtttacc
    22441 actcccgaca tccgctggcg gtgcgttcag atattgagaa gcgactggac cgcgctttta
    22501 ctcgtcataa cgaactcgat ttctggaaca acgaagatat cgcagatgcc ttacataacc
    22561 gtccggaatc gcaccatctg tttgtggtgt tggggacatc ggttatagaa gtggggcgtg
    22621 actgggatgc ggactgggga attatcgaac caagctccat gcgttcgata attcaatttg
    22681 ccggacgtat tcagcgccac cgtagaaatg tgccgaccag tgagaatctg gttattttgc
    22741 gtagcaatat caaatcattg cagaaaaaag cccccgcatt ttgtaagcct ggtttcgaaa
    22801 caaaagaaca cttgctgaat agccacgata tttgcgaact gattccgatc gcggactacc
    22861 ggacgattaa cgccttgccg cggatcctgt ctgctaataa tgccaacaaa ctggcggagc
    22921 tagaacatag tcgtttatcc gccgaattga tgagcagtaa aagcaaacat gtggtggcgg
    22981 cgcaatggtg gcgtgcaccg ctaagctgga acggctggat gcagcgccag acgccatttc
    23041 gttattcccc tttgccggag gcggtgtttt ttctgcatat ggatgatgaa gaaagcgaag
    23101 cgcgtttcta ttcccgcacg ggcgacaacg agcgtaaagc gcaagggaat ttcaggcgag
    23161 aagaggtgag atgcgccagc ggcgtcgctt gctggggcgt gatggattac caacacgttt
    23221 tgttgaattt agctgataag caaagttgtg agatagcaaa cgttggggaa aagtttgcgg
    23281 aggtgagagt acccgtcagt gaagaagatg cggtcgatga ctggcgttat cacccctggt
    23341 taggtgtgtt tcgcaacatg tgatggggcg cgagctgcct gtacggcagt ttacaagtcc
    23401 gtatccggag atacctctat ttctaagctg ctcaaggcag tttgggtatg ttagcgcttc
    23461 aactattgcg tggcaaatat taagttgctt taatatcacc tatggtgatt acatgagatt
    23521 taataatatc ttttagaaga tatctctagg agagtttgtt tgtgtggtca ccactggtgt
    23581 tgtccctttt gctgtgtatg tcagttgtac tgaagccatg ttgggctacg gagatttttc
    23641 agtgaaatca gggagcaact tcgacttgat ggattaacga aaaggagccg atatggaaaa
    23701 aacggcgcta atacaattta ttactgacta catcgcttcg cgaaggcaac ccaaaataga
    23761 tgcctttgaa aaagaagcag caaagcggtt ggaacagggc gaggacgcca gcgttatcgc
    23821 tctggagcga caggaactcg aagcacgcta cttgccacgt aactggttaa cggatgcggc
    23881 aaagcgtgcc gggcaaatca aattagtcac tcatggcgct aaattcagtc atggtgattc
    23941 taaagccagt agcttttatc tggaaacctc cgctaacgaa agctatttaa acacagcatc
    24001 gttggcgaat gtcgctactg acgcgattgg taacgccgct gcacttgacg tagcaaagtt
    24061 gctacaaacc gatgtgaacg gagattcact gcttgccagc ctgaagcgtg gtgattatca
    24121 ggcattaagt acgtttgcag aggataaagt gcaactggag ctgtgggtag ctgggtttag
    24181 ccaggcatgg actactggac ggccttccag ccataaactg gcgaaacaaa tctatttccc
    24241 tgttgcggat ggttatcacc tgttatgccc actgttttcc agttcactgg cgcaggcgat
    24301 gtatgaaaag ctaaccgccg tgcgctttag tgaggagtcg aaagccattc gcgatgcccg
    24361 caaagcagga aaatggcact cccagccaga tatctggttt cctaatcttg ccgaaatgca
    24421 ttttggcggc acaaaaccgc agaatatttc tttgctcaat agtgtgcggg gtggccgtgt
    24481 ctggctgttg ccgtcaatgc cgcccacctg ggcgacgctg gatcgcgcgc cacaaaacat
    24541 gcgcagtatt ttttccctac gcggcgattt caatcgggcg gcttcaggca ttgtcgccca
    24601 gatgacctac ttactgaagg ttgacataaa taatgtccat atccgaactg tgcgggcgaa
    24661 atatattgat gagttgatcg atttgctgtt catgcaggca tccgcttttc agcaagaaaa
    24721 atggcaggga tggtccgcac aatcaccaga cttacctcgc caccaacaac tttggctgga
    24781 cccgtggcga agcctctcag atgagacttt caaacaggag cgtgagaaaa acgactggca
    24841 agtaactgtc gccgatgatt tcgcacgctg gctgaactat cgcctgaaaa aatccagttt
    24901 tgacgtaggc gctgtcgaac aaaaagagtg gcgcagccag tctctgttcg ctcaacggat
    24961 gcgtgaaatg gaagctgttt tgcaggaggc gctgaaatga gttatctgct cttgttaccc
    25021 catatacgca tcgaaaacgc taacgctgtc tccgggttga cctggggatt cccgtcgatg
    25081 acccattttc tcggttatgt ccatgcgctt tctcgcaaag tcgtggatga atttggcgta
    25141 agttttgatg gttgtgcggt agtaagccat gaacagcata ttcaggcgta cagctctggg
    25201 cgcgattttc agttcgctct cacccgtaac ccattaacca gagaaggtaa aaccgcctcg
    25261 ttcaatgaag agggccgcat gcacttgacg gtttctctac tgattgaatg taatggcgaa
    25321 atcaccaatg gtgaatatgg cagaaaagtt ctttgtgact atctgaaaat gctttgccag
    25381 agccacaaac tggcgggcgg cagcattgtc agtatgcgcg atccgcagct ttttcatgcg
    25441 cctgaagatg aaaagcaact gcgtaaaatc gtctggcggt taatgccggg ttatgcgctt
    25501 tacgatcgca gtgaatggct ggctgaacat caccaacaac atcccgacat ctcgttgctg
    25561 gatgcctggc ttgatttcgc cgcgattaaa tatcaggccg aatctccggc tgaagacaac
    25621 agcgcaaaat gggtatacca gccgaagcca atgcctggtt tcctggtacc gttaatgtgt
    25681 ggctaccaac gcatttctcc tgtttacgca cccggtgaag ttgaaaatgc gcgcgacacc
    25741 gtgacgccat ttgcctttgc cgaagcggta tatggcattg gcgaatggcg cggattacat
    25801 cgcactacag acttgcaagc gctgatgtgg cgttaccgca caacggacac tggctactat
    25861 tgttcggcaa cgccagttgt tgatgatttt acctttaatg aatatgacga tttagaataa
    25921 ggacataatt atggcaaagg ctcctgtagc aataaaaacg gcgtcggtac tggcatttga
    25981 gcgcaaactg gcaacctctg acgcggtaat gtacgcaggt aactggcaag gaaatgaatg
    26041 gcaacccatt gagattcagg agaaagcggt ccgcggaacc atctctaatc gcctgaaaaa
    26101 tgccattgtc agcgatccca caaaactgga tgctgaaatc caaaaagcca acctgcaacg
    26161 ggtggatgtg gcttcgcttc cggtagatac ggatacgtta aaggtggtgt ttacgctgcg
    26221 tgtgctgggt aatttatcca caccgtcagt atgtaatgat atggcttacc aggaggcgct
    26281 ttccggggtc attgaaggtt acatcagcga acatggcttt aaagagctgg cgcttcgcta
    26341 tgcgctgaat ctggcaaatg gtcgttttct gtggcgcaac cgcattggtg ccgaacagat
    26401 ccaggtgaaa gtgactgcta acgagacttc atggactttt aacagccatg acttctcttt
    26461 acgtcaattt gaccaaggac aggcagcggt cacgcagctg gcggccatta tagagcaagg
    26521 attaagcgca aaagagtggg tgatgcttac cgttgaagcg caggttcgtc ttggtgccgg
    26581 gcaagaagtt tttccgtcgc aggagctggt actcgacagc aacagtagta aaagtcgcgt
    26641 gttataccaa gtggcaggca ttgccggtat tcactctcag aagattggca atgctttacg
    26701 taccattgat acctggcatc caaaggttga tgaattgggg gctatagccg tggaacctta
    26761 cggttctgta accagtcgcg gtgtggcctg ccgtcaaccg aaagaaaaac tcgactttta
    26821 taccttactg gacaattggg tgactaaagg gatgaaacca gacgttaagc agcagcacta
    26881 cgtgatggct gtgctgatcc gtggcggtgt ctttggtgag aaatcggaat aaggagctgc
    26941 caatggatca ctaccttgag atccgcgttc tgccagatcc tgaattttcg agcgaaatgt
    27001 tgatggcggc gttatttgca aaactgcatc gggtattagg tgcaagaggg caaggcgata
    27061 tcggtgtgag tttccctgac gtaaatgtta tgcctggagc gcgtttacgc ttgcatggta
    27121 gcgcccaggc attgcaggcg cttgaagcct caacgtggcg taaaggacta acggactatt
    27181 gccaatgttc acctgtcact cccgttccag aaataaaggg ctggcgagta gtaagccgtg
    27241 tgcaggttaa aagtaactcc cagcgtctgt taaggcgttc agtgaaaaag ggatggctaa
    27301 ctgaagaaca ggcaattgaa cgactggcga cgcaggctga acagcggact gatttacctt
    27361 ttctcaatat gaagagtttg tcgagtcagc aactgttcaa gttgtttatc cgtcatggtg
    27421 atttgctcaa agaaccggtt aaaggggaat ttagtagtta tggattaagc gcaaccgcca
    27481 ctattccctg gttttgatgc tttgcaattt ttaacgggcc tgaccatgcg gttgggcctt
    27541 ttttgtgtag gccatgtctg gtgcaaaaaa aagcccgtac tttcgtacga gctcctcttt
    27601 aaagatggcg gtgaggcggg gattcgaacc ccggatacgt tgccgtatac acactttcca
    27661 ggcgtgctcc
//
LOCUS       DADSZD010000049        27144 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.049, whole genome shotgun sequence.
ACCESSION   DADSZD010000049 DADSZD010000000
VERSION     DADSZD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.049"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            92..1435
                     /locus_tag="J1Z28_001848"
     CDS             92..1435
                     /locus_tag="J1Z28_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBA7716687.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEGYEQQGGFNETQAQEFVQEALETFRWHLSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            1660..3315
                     /locus_tag="J1Z28_001849"
     CDS             1660..3315
                     /locus_tag="J1Z28_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HBA7716688.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            3455..3679
                     /locus_tag="J1Z28_001850"
     CDS             3455..3679
                     /locus_tag="J1Z28_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBA7716689.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            3741..4280
                     /gene="rimL"
                     /locus_tag="J1Z28_001851"
     CDS             3741..4280
                     /gene="rimL"
                     /locus_tag="J1Z28_001851"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBA7716690.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(4272..5252)
                     /locus_tag="J1Z28_001852"
     CDS             complement(4272..5252)
                     /locus_tag="J1Z28_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBA7716691.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAMSDFNDVIAGSAG
                     GWIDHETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRAKV
                     SASRIVHQAQIYGDAVARYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVCGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTIHVRGPKVINGEERITRTLLAGLL"
     gene            5376..6368
                     /gene="tehA"
                     /locus_tag="J1Z28_001853"
     CDS             5376..6368
                     /gene="tehA"
                     /locus_tag="J1Z28_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBA7716692.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGGLPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            6365..6958
                     /gene="tehB"
                     /locus_tag="J1Z28_001854"
     CDS             6365..6958
                     /gene="tehB"
                     /locus_tag="J1Z28_001854"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBA7716693.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLITNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            7261..7929
                     /locus_tag="J1Z28_001855"
     CDS             7261..7929
                     /locus_tag="J1Z28_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBA7716694.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(7964..9142)
                     /gene="ydcO"
                     /locus_tag="J1Z28_001856"
     CDS             complement(7964..9142)
                     /gene="ydcO"
                     /locus_tag="J1Z28_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBA7716695.1"
                     /translation="MTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQ
                     ISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVT
                     NVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVW
                     LATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPDFSFAHSLSVAL
                     PLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALFFSPFGVYSVGIAAITAAI
                     CQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTI
                     GGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCYVVLNLIADRNR
                     "
     gene            9228..9764
                     /locus_tag="J1Z28_001857"
     CDS             9228..9764
                     /locus_tag="J1Z28_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7716696.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPLQSSTPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVFVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            9837..11798
                     /gene="rlhA"
                     /locus_tag="J1Z28_001858"
     CDS             9837..11798
                     /gene="rlhA"
                     /locus_tag="J1Z28_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBA7716697.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQIGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHIDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(11890..12120)
                     /locus_tag="J1Z28_001859"
     CDS             complement(11890..12120)
                     /locus_tag="J1Z28_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
                     /protein_id="HBA7716698.1"
                     /translation="MFSKALSVVLLTCALFSGQLMAGHKGHEFMWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            12470..13876
                     /locus_tag="J1Z28_001860"
     CDS             12470..13876
                     /locus_tag="J1Z28_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA7716699.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQVVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            14121..15266
                     /locus_tag="J1Z28_001861"
     CDS             14121..15266
                     /locus_tag="J1Z28_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7716700.1"
                     /translation="MSKIFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHNLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            15284..16297
                     /locus_tag="J1Z28_001862"
     CDS             15284..16297
                     /locus_tag="J1Z28_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7716701.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            16298..17239
                     /locus_tag="J1Z28_001863"
     CDS             16298..17239
                     /locus_tag="J1Z28_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7716702.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            17229..18023
                     /locus_tag="J1Z28_001864"
     CDS             17229..18023
                     /locus_tag="J1Z28_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7716703.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            18045..19469
                     /gene="patD"
                     /locus_tag="J1Z28_001865"
     CDS             18045..19469
                     /gene="patD"
                     /locus_tag="J1Z28_001865"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBA7716704.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(19566..19661)
                     /gene="yncL"
                     /locus_tag="J1Z28_001866"
     CDS             complement(19566..19661)
                     /gene="yncL"
                     /locus_tag="J1Z28_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBA7716705.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            19856..20029
                     /locus_tag="J1Z28_001867"
     CDS             19856..20029
                     /locus_tag="J1Z28_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HBA7716706.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            20115..20348
                     /locus_tag="J1Z28_001868"
     CDS             20115..20348
                     /locus_tag="J1Z28_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBA7716707.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(20349..20798)
                     /locus_tag="J1Z28_001869"
     CDS             complement(20349..20798)
                     /locus_tag="J1Z28_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7716708.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(20795..21313)
                     /gene="mddA"
                     /locus_tag="J1Z28_001870"
     CDS             complement(20795..21313)
                     /gene="mddA"
                     /locus_tag="J1Z28_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBA7716709.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            21494..21562
                     /locus_tag="J1Z28_001871"
                     /pseudo
     CDS             21494..21562
                     /locus_tag="J1Z28_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase yncb"
     gene            21562..21717
                     /locus_tag="J1Z28_001872"
                     /pseudo
     CDS             21562..21717
                     /locus_tag="J1Z28_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(21794..23896)
                     /locus_tag="J1Z28_001873"
     CDS             complement(21794..23896)
                     /locus_tag="J1Z28_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7716710.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTEAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNVYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMAFASIGYIPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            24138..25199
                     /locus_tag="J1Z28_001874"
     CDS             24138..25199
                     /locus_tag="J1Z28_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBA7716711.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTTNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(25312..26811)
                     /gene="ansP"
                     /locus_tag="J1Z28_001875"
     CDS             complement(25312..26811)
                     /gene="ansP"
                     /locus_tag="J1Z28_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBA7716712.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVFAIVTFLVVGTVFLGSGQPLDGNATGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPVIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
BASE COUNT         6838 a         6676 c         6942 g         6688 t
ORIGIN      
        1 ttcgcggcga gatcacagtt tgtaaattct tccagccgga gtgaatgcgg ttacctacac
       61 tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg cggatgagat
      121 tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc cgcagtacgg
      181 cacgctgctg gaactggtag ctgatgtgaa tctggctgtg ctggaaaaca atcctcaact
      241 gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac gtcatggggc
      301 gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg ccattatggg
      361 gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac attcgacagc
      421 atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt ttacctcgtt
      481 actccgtctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg agattctgcg
      541 tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gagggatatg agcagcaggg
      601 cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg
      661 gcacctgtca gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt
      721 gattgctgat gtggtctgtt ttcctggatg ccatatcaac catctgacgc cacgcacgct
      781 ggatattgac cgggtgcagt cgatgatgcc tgaatgcgga attgaaccca aagttctgat
      841 cgaagggccg ccgcgccgcg aggtgccgat tttactacgc cagaccagct ttaaagcact
      901 ggaagagacg gtgttgtttg cggggcagaa acagggcacg cataccgcgc gctttggtga
      961 aattgagcag cgtggtgtgg cattaacgcc gaaagggcga caactgtatg atgatcttct
     1021 gcggaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac aggaaacctt
     1081 ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggctgggat ggttccggta
     1141 ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcccggag acgatccaca
     1201 gcccttaata gaacgtggtt gggtagtggc gcaacccatt acctatgaag atttcttgcc
     1261 cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcagg catgcaatca
     1321 cggtgatgcc agtcgcgaag catttgagca ggcgttgggg tgtccggttt tggatgagtt
     1381 ccagctttat caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc tttaaattct
     1441 gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct catcccatcg
     1501 acataaaaaa aatgccaatt tatgcatatt ctctgatttc aacaattgga ttattaataa
     1561 atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa atgaccagcg
     1621 cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag acgatttatt
     1681 aaaggttcaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct
     1741 caggcggcat tcgcggccga ttctgatatt gccgacgggc aaacccagcg ttttgacttc
     1801 tccattctac agtcaatggc gcacgactta gcgcaaacag catggcgtgg tgcaccacgt
     1861 ccgttacctg acactttggc gacaatgacg ccgcaggctt ataacagtat tcaatacgac
     1921 gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca gtttttccat
     1981 atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc aacacatctg
     2041 gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcggg tgtggatacc
     2101 aaacaattag aagggcaaag cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc
     2161 gaactggcgc gccgtgatgt agtatcattc ctcggcgcga gttatttccg cgccgttgat
     2221 gacacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta caccgacagc
     2281 aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc aggggcaacc
     2341 acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta taagttcact
     2401 atccattgtg agaaaagtca ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa
     2461 gacattaaac agctgggcat tgcgccgatg accagcatgt tcagctgcgg tactaatgaa
     2521 cgtcggatgt gcgacaccat tcatccgcaa atccatgact ctgatcgtct gtccatgtgg
     2581 cggggcaacg gcgagtggat ttgccgtccg ctgaataatc cgcaaaaatt gcagttcaat
     2641 gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg tgatttctcc
     2701 cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt
     2761 aacaagtggg gtaagggcac catcggcctg atggaaatcc caactacggg ggaaacgctg
     2821 gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcgggtga tgagtttgca
     2881 ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt
     2941 atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg tgaacactat
     3001 cccgaaaaat gggcgcgacg ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc
     3061 gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa
     3121 attctctata ttgaacccat tgatggttat cgtattcagt ttgactggta tccgacttcg
     3181 gactccactg atccggtcga tatgcggatg tatctgcgtt gtcagggcga cgctatcagt
     3241 gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta tgttgatgac
     3301 cgcgtgatga gttaatcgtt ttttcttcgg catcttcttc gggaggtgcc gtctggttaa
     3361 acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt gtgtatttca
     3421 gatacacctt gtcagccact aacagggagt gcgtatgttt ccagaatacc gagatttaat
     3481 atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac acaataaact
     3541 tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg cggaagtggt
     3601 ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc tgcagcaggt
     3661 gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt ttaaagctgc
     3721 atggattgac tggagataag atgactgaaa cgataaaagt aagcgaatca cttgaattac
     3781 atgctgttgc ggaaagtcat gtcacacctc tttatcagtt aatctgtaaa aataaaacct
     3841 ggttacagca gtcgctaaac tggccgcagt ttgttcaaag tgaagaggac acgcgaaaaa
     3901 cggtgcaggg taatgtgatg ttgcatcaac gcggctatgc caaaatgttc atgattttca
     3961 aagaagatga acttatcggc gttatctctt ttaatcgtat tgaaccactg aataaaaccg
     4021 ctgaaatcgg ctactggctc gacgaatctc atcaggggca ggggatcatt tctcaggcgc
     4081 tgcaggcatt gattcatcat tacgctcagt ctggtgaact tagacgcttc gtgatcaaat
     4141 gtcgggtgga caatccgcaa agcaaccagg tagcgttgcg caatggtttt atccttgaag
     4201 gttgcctgaa acaggctgag tttctgaatg atgcctatga tgatgtgaac ttatacgcgc
     4261 gtattatcga ttcacaataa ccctgccagc agcgttcgcg taatgcgctc ctcgccgtta
     4321 ataacttttg gaccacggac atgaatcgtg tcgccatcaa aggcttcaac gacggcgtgg
     4381 tcggttaact ccacatggtt ttcaattatc accgcacctg taatacggct ttcaccttgt
     4441 attacaacgt gctcgtcgag cagaatcggt ccgccgcata ccacggcatt tccgccaatc
     4501 aagacatgat gttttaacac acaattacct tccacaatgg catattccgc cacctgcgaa
     4561 ctgtaatgaa tcgtcggaat ggcatcttct tctatgccag ctttcacctg cgcgtgaccg
     4621 tagactttag cgcaatcgca tagccagacg ttgttctctt cattaccttc aacgctggca
     4681 aaatcaaaaa cttcggcgcg atgttcaata aaagcatacc gggcgacggc atcgccataa
     4741 atttgtgcct ggtggacaat acgcgaggcg ctaacctttg cgcgatcata aatttgtagc
     4801 aggagttgat ggtcgggcgt taagccttgt gcggcgacga tcatagaatg ttgatcaatt
     4861 aaggcgtgac caaatattct acattgacca caaactaatg agtcacgaat ggtgacactg
     4921 tcacttatat atgcaccctg gctgatttca ctattatcga tccagacatt atccgttgcg
     4981 taaacctctc cccacagcac gctggtgccg gtgatacgcg tattcccgga aatcaccgtt
     5041 ccgccgaacg caatagcatt ctggtcgtaa atccagcaat ttccttcctg tgctaacacc
     5101 gtttcgtgat cgatccagcc gccagcgctt cctgctatca catcgttaaa atcgctcatg
     5161 gcgataattt gccgtaataa cacacttttt ttagtgccat cttcctgata actaaaggct
     5221 cgctgttctt cacttagacg atatttacgc atagcgtttt cccacaggtg acttactata
     5281 accgtagcaa attctgcggc tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa
     5341 atatacttta taaattaaac aaaatgagta agaagatgca gagcgataaa gtgctcaatt
     5401 tgccggcagg ctactttggt attgtgttgg ggacgatagg gatgggattt gcctggcgct
     5461 atgccagcca ggtttggcag gtcagccact ggttgggtga tgggctggtg attctggcga
     5521 tgatcatctg gggattattg actggcgcat ttattacccg actcatacgc tttccgcata
     5581 gcgtgctggc agaagtgcgc catccagtgc tgagcagttt tgtgagtttg tttccggcaa
     5641 cgacgatgct ggtggcgatt ggttttgttc cgtggtttcg cccactggcg gtgtgcctgt
     5701 tcagttttgg tgtcgtggtg caactggctt atgccgcctg gcaaactgcg ggattatggc
     5761 gcggatctca ccctgaagaa gctaccacgc ctggactgta tctgccgaca gttgccaaca
     5821 actttatcag cgcaatggcc tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt
     5881 taggcgcagg cgttttctca tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca
     5941 gttcgggagg attacccacg gcactgcgga catcactcgg cattcagctc gctcctgcgc
     6001 tggtggcctg tagtgcctgg ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa
     6061 tgcttttcgg ttatggactg ctgcaactgc tgtttatgct acgtctgatg ccatggtatc
     6121 tctcccagcc atttaatgct tcattctgga gtttctcgtt cggtgtatct gcactggcaa
     6181 ccaccggttt gcatctgggg agtggcagcg ataatggatt tttccatacg ctggcggtgc
     6241 cgctgtttat ctttaccaat tttattattg caatactgct catccgtact tttgcgcttc
     6301 tgatgcaggg aaaattgtta gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca
     6361 aagaatgatc attcgtgatg aaaactattt tactgataaa tatgaattaa cccgcacaca
     6421 ctctgaagtg ctggaagcgg tgaaagtggt taaaccgggt aaaacgctgg atctgggctg
     6481 tggcaatggt cgtaacagtc tttacctggc agccaatggt tatgatgttg acgcatggga
     6541 taaaaatgcc atgagtatcg ccaacgtcga gcgcattaag tccattgaaa atctggataa
     6601 tttacacacc cgggtcgttg atctgaataa cctcacattt gatggacagt acgattttat
     6661 tctttcgact gtggtgctga tgttccttga ggctaaaacc atcccagggc tgattaccaa
     6721 tatgcaacgt tgcactaaac ctggcggtta caacctgatt gtggcggcga tggataccgc
     6781 tgattatcca tgtaccgtcg gcttcccgtt tgccttcaaa gagggggaat tacgtcgata
     6841 ttacgaaggc tgggagatgg tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga
     6901 cgccaacggt aatcgtatta aactgcgttt cgccacgatg ctggcacgta aaaaatgatc
     6961 cggtaagaac aaagcgcgtg aaaattcccc acgctgagat ggtttactgt tcttcttttc
     7021 ggtaagcata ttttttatcg aagggatgtg aaattaatca cagtagtcga agttttttag
     7081 ccgcttaact tactgaaatt taactactga tgattgactt agcccctttt tcggcattga
     7141 ctatgtcgtc tgaaaagggg ctaaaaaatt tattttcacc aacacttttt ttgccacaac
     7201 acgaagcggc gctttttgct ataacttaga aagtaatata atcatctcag gaaactattc
     7261 atgcgtacca catcatttgc gaaagttgca gctttatgcg gcttgttggc tctgtctggt
     7321 tgtgcctcta aaatcaccca gccagataaa tattctggtt ttttaaacaa ttactctgat
     7381 ttaaaagaaa caacctcggc aacaggtaaa cctgttttac gttgggtaga cccgagtttt
     7441 gatcaaagca aatatgacag catcgtctgg aacccaatca cttattatcc ggtaccgaaa
     7501 ccttctaccc aggtagggca gaaagttctg gataaaattt tgaactatac caacaccgaa
     7561 atgaaagaag cgatagcgca gcgtaaacca ctggttacca ccgctgggcc gcgtagtctg
     7621 attttccgtg gggccattac cggtgtagat accagtaaag aagggctgca attctatgaa
     7681 gtggttcctg ttgcattagt ggttgcgggg acgcaaatgg ctacaggcca ccgtaccatg
     7741 gatactcgct tgtattttga aggtgagctg attgatgcag cgactaataa accggttatc
     7801 aaggtcgttc gtcagggcga aggtaaagac ctgaataacg aaagtacgcc aatggctttc
     7861 gaaaatatta aacaagttat tgatgacatg gcgaccgatg ccaccatgtt tgacgttaac
     7921 aaaaagtagt tccagacgcg ccatcgttag atggcgcttt ttattatcgg tttctgtcag
     7981 cgattaaatt caacaccacg taacaaacgc ctccggcaat taatccccaa aacgcagaac
     8041 caatcccgaa cagcgtcaat ccacttgccg ttaccagaaa tgccaccacc gccgcgtcgc
     8101 gctcacgctc attatgcagc gcctgataca aactgccgcc aatggtactc aacagcgcaa
     8161 gacctgccag catctggatc caacttacgg gcagggcagc catcatcccg gtaatggcac
     8221 taccaaacag acctgcgatc aaataaaaaa taccagcaac ggcagcggcc agccaacgtt
     8281 gatctttatc cggatgcgct tccgggcttt ggcaaatagc cgcggtgatt gccgcaatac
     8341 cgacggaata aacgccgaaa ggggaaaaaa acagtgccag caatccagta aacacaatca
     8401 acggcgaaac aggagccgaa tacccggctg ctttcattgc tgcgataccc ggtgcgtttt
     8461 gcgatgccat cgtcaccaga aaaaggggga gtgcaacgct cagactgtga gcaaacgaaa
     8521 aatcaggggg aatataagtg gggagaacgg gtttaaagac aacatcagtt gtgacaacgt
     8581 caccttgcgc gataacgatc acgatcccaa taatcatcgc ggcaattacc gcatagcgcg
     8641 gcgcaacggc cctggttgcc agccatacca gcaacatact gccacacaac gtaaattgac
     8701 catccagact ggcaaacgcc tgtaaaccaa aacgtaataa aatcccggca agcattgccg
     8761 ccgcaagcga gtgcggaata atgcgcatca gacgagcaaa gagtcccgtt atgccgcaga
     8821 ggactattaa cacgttggtg acaataaaaa cgccgatggc ttcgttaagt gttagtccct
     8881 gtaatccggt gaccaacaaa gccgcaccag gcgttgacca tgcggtgaga acaggtacgc
     8941 gataccataa tgtcagagcc agcgtactga cgcccattgc cagccccagc gccgtcatcc
     9001 agccagagat ttgtgcagtg gtggctccgg cgacaatcgc tgcttgccag attattgccg
     9061 ctgaactggc gtagccaatt aataccgcca gaaaccccgc cagtagcgtg ggtggaggaa
     9121 tagaaaacag acgcatagtc acctcgtgcg ttataacgta cggctaacgt agcatttgtc
     9181 cgttataacg cacaagtgat aaacttccgt tttgccggag gagtcgcatg gaaaatctcg
     9241 ctcgcttttt atccaccaca cttaaacaac tacgtcagca gcgcggctgg agtctttcgc
     9301 gactggcaga agcgacgggc gtttctaaag cgatgttagg gcagattgag cgtaatgaat
     9361 ccagcccgac ggtcgcgaca ttatggaaaa ttgccaccgg gttgaacgtg ccattttcaa
     9421 catttatttc tccgctgcag tcctctacgc cttcagttta tgatccacaa cggcaggcga
     9481 tggtgatcac ttcgctgttt ccttatgatc cacaactctg ctttgaacac ttctcgattc
     9541 agatggcacc cggtgcaata agcgaatcga cgccccatga aaagggggta atcgaacatg
     9601 tcttcgtcat tgatggacaa cttgatctgt gtgttgatgg tgaatggcag agccttaatt
     9661 gtggggaagg cgttcgattt gctgcagacg tcacgcatgt ctatcgtaat ggtggggagc
     9721 aaaccgtaca ttttcattcc ctcatccatt acccgcgcag ttaagcggga aaactatttc
     9781 gcaacgtcgc gcttgtggct aaaatagccg ccatttttca gctactggat aagaaagtga
     9841 ccgtatcttc tcatcgtctt gaactgttaa gcccggcacg cgatgccgcc attgcccgcg
     9901 aagctatttt gcacggtgcc gatgctgttt atatcggcgg ccctggtttt ggtgcccgtc
     9961 ataatgccag taatagcctg aaagatattg ccgagttggt gccgtttgcc catcgttatg
    10021 gtgcaaaaat tttcgtcacg cttaacacca ttttgcatga tgatgagctg gaacccgcgc
    10081 aacggctgat tactgacctc taccagatcg gtgtcgatgc gctgattgtt caggatatgg
    10141 ggattctgga acttgatatt ccgccgattg aactgcacgc cagtacgcag tgcgatattc
    10201 gtacagtaga aaaagcgaag ttcctctctg atgttggctt cacgcagatt gtgctggcgc
    10261 gagagctgaa tcttgagcag atccgcgcga ttcaccaggc cacggacgcg accattgaat
    10321 tctttattca tggcgcattg tgcgtggcct attcgggtca gtgctacatt tctcatgcgc
    10381 aaacagggcg tagcgccaac cgtggcgatt gctcgcaggc gtgccgtttg ccatacacat
    10441 tgaaagacga tcaggggcgg gtggtttcct atgaaaaaca tctgctgtcg atgaaagata
    10501 acgatcagac tgccaacctc ggcgcgctga ttgatgctgg cgtacgctcc ttcaagattg
    10561 aagggcgtta caaagatatg agctacgtga agaatatcac cgcccattat cgtcagatgc
    10621 tggatgccat tattgaagaa cgtggcgatc tggcgcgcgc ttcatcaggt cgtactgaac
    10681 atttctttgt tccatcgacg gaaaagactt tccaccgtgg tagcacagat tattttgtga
    10741 atgcccgtaa aggcgatatt ggcgcgttcg attcgccgaa atttatcggc ctgccggtgg
    10801 gcgaagtatt gaaagtagcg aaagatcata ttgatgttgc cgttaccgag ccactggcaa
    10861 atggcgatgg cctgaacgtg atgattaaac gtgaagtcgt cggttttcgt gctaatacgg
    10921 tagagaaaac cggagaaaat cagtaccgcg tctggccaaa tgaaatgcca gcagatttgc
    10981 acaaaattcg cccgcatcac ccactaaacc gaaatcttga tcataactgg cagcaggcac
    11041 tgacaaaaac ctccagcgaa cgtcgggtgg cggtagacat tgaactgggc ggctggcagg
    11101 aacaactgat tctgaccctc accagtgaag agggtgtcag catcacacat acgctggacg
    11161 ggcagttcga cgaagccaat aacgccgaaa aagcaatgaa caatctgaag gatggtctgg
    11221 caaaactggg gcaaaccatc tattacgccc gcaatgtgca aattaatttg ccgggggcgc
    11281 tgtttgtacc aaacagtctg ttaaaccagt tccgccgtga agccgccgat atgcttgatg
    11341 ctgcgcgtct tgccagttac cagcgcggca gccgtaaacc ggttgctgat cctgcgccgg
    11401 tttatccgca aacgcatctg agtttcctcg cgaacgtata caaccagaaa gcgcgtgaat
    11461 tttatcatcg ctatggtgtg caactgattg acgcggcgta tgaggcacat gaagagaagg
    11521 gcgaagtccc ggtgatgatc accaagcatt gtctgcgctt tgccttcaat ctgtgcccga
    11581 aacaggcgaa aggcaatatc aaaagctgga aggcgacgcc aatgcaactg gttaacggcg
    11641 atgaagtatt aacgctaaag tttgattgcc gcccatgcga gatgcacgtc attggcaaaa
    11701 tcaaaaatca catactgaaa atgccgttac cgggaagcgt agtggcatcc gtaagtccgg
    11761 atgatctgct gaaaacattg ccgaagcgaa aagggtaaaa cgccagtttt cgggttactc
    11821 accactcatc aaatctgcat gatattgcct gccgggtaag gcgtcatgcc gcatccggca
    11881 acaaatgact caacgctgtc ccgcttctgg tttgcgcgat ttttgccagt aaaaatgctc
    11941 gcgcaaacct tccgccgact cttccgccac agcacgcaat tcatcgctgt ccgcttcatg
    12001 acgcagctga tgatccacat tctttaccca cataaattca tgtcctttgt gccctgccat
    12061 gagttgtcct gagaacagtg cacacgttaa taagacaacc gataacgcct tcgaaaacat
    12121 cccgccacct ttttctaacc ttttgccgct atgatgccga tcgtttcttg aggttattat
    12181 tcagttttgc aaattagagc aaagaaattc tggaatcttc cttcctgatt ttgcattgca
    12241 ttctgccgtt gcggcgattt agtgctattt tcgagcacat tacacacgga ggtaaacttc
    12301 gccattaaat agtcacgatc actttattga agtgcctttg agcgtcgcct ctaaggcgct
    12361 tggcaaagag ttatcgctgg tgatggttta attacagtta acgaaaagtt gtcattttta
    12421 acaactgata tagactgccg aatcatctgc acataattac gattcgataa tgaaaaaata
    12481 ccagcagctt gcagaacaat tacgcgagca gattgcgtcg ggtatctggc aacccggcga
    12541 tcgtttgcct tcgttgcgtg accaggtggc gctttctggc atgagcttta tgactgtcag
    12601 ccatgcctat cagttgctcg aaagtcaggg atatattatc gcgcgaccgc aatcgggtta
    12661 ttacgttgcg ccacaggcaa taaaaatgcc gaaagcgaca gtcattccag tcacacgaga
    12721 tgaagcagtc gatatcaaca cctatatttt tgatatgttg caggccagtc gcgatccgtc
    12781 ggtagttccg tttgcctcgg cctttcccga cccgcgactt ttccccctcc aacaactaaa
    12841 ccgctcgctg gcgcaggtaa gcaaaaccgc cacggcgatg agcgtgattg aaaacttacc
    12901 gccaggaaac gcagaactgc gtcaggctat tgctcgtcgc tatgccttac agggcatcac
    12961 catttctcct gatgaaattg tcattactgc cggggcgtta gaggcattaa acctcagttt
    13021 gcaagtggta actgaaccgg gcgattgggt gatagtagag aatccttgtt tctacggcgc
    13081 gttgcaggcg ctggagcggt tacggctgaa ggcgttatcg gtggcgacgg atgttaaaga
    13141 agggatagat cttcaggcgc tggaactggc gttgcaggag tatccggtga aagcgtgctg
    13201 gctgatgact aatagccaga acccacttgg atttacctta acgccgcaga aaaaagtgcg
    13261 gctggtagcg ttgctcaatc agtacaacgt aacgctgatt gaagatgacg tttatagcga
    13321 actttatttt ggacgggaaa aaccgctgcc tgcgaaagcg tgggatcgcc acgatggtgt
    13381 tttgcattgc tcttcgtttt cgaaatgtct ggtgcctggt tttcgtattg gttgggtcgc
    13441 cgccggaaaa catgcacgta aaattcaacg cttgcagttg atgagtacgc tttccaccag
    13501 ctcaccgatg cagcttgcgc tggtggatta cctttctact cgccgatacg atgctcatct
    13561 tcgtcgcctg cgtcgccagc ttgcggaacg taaacaacgc gcctggcagg ctttgctgcg
    13621 ttatctgcct gcggaagtca aaattcatca caatgacagc ggttattttc tctggctgga
    13681 gctccccgag ccgttagatg caggagaatt aagcctggtg gcactgacgc atcatatcag
    13741 tattgcgccg ggtaatatgt tttctaccgg tgaaaactgg tcacgttttt ttcgttttaa
    13801 taccgcgtgg cagtggggag aacgtgaaga acaggcggta aaacaattag gcaaacttat
    13861 tcaagaacgg ctgtaatagc gtttaattta attcctctca ggttgggtaa tatgaatttc
    13921 gaatagcagt catatatcct aactccttgt ctatactcca gaagataacc ttacagaggg
    13981 cataatgcgc ggtagctcac aacctgatta aattttctca gggccgaagg tgtgcctgct
    14041 agccgccgtc tatggttaaa caaggagata tttttacggc acggcggctg aacaattaat
    14101 tacgacagga gtaagacctt atgagcaaga tatttgcccg cagcagcctg tgtgcgctca
    14161 gcatgacaat aatgaccgct cacgccgccg aaccgcctac caatttagag aatccggaag
    14221 ggcgactgga tattatcgcc tggccgggat acatcgaacg cggacaaact gataaacagt
    14281 acgactgggt aacgcaattc gaaaaagaga caggctgcgc ggtgaatgtt aaaaccgccg
    14341 cgacttccga tgaaatggtc agtctgatgg ccaaaggggg ttacgatctg gttacggcat
    14401 ccggcgatgc ctcgctgcgt ttgattatgg gcaaacgcgt gcagccaatt aataccgcct
    14461 tgattcccaa ctggaaagcg ctcgatccgc gcgtggttaa aggcgactgg tttaacgttg
    14521 gcggcaaagt ttacggcaca ccttaccaat gggggccgaa cctgctgatg tacaacacta
    14581 aaaccttccc gacgccgccg gatagctggc aagtggtttt tgttgagcag aatctgccgg
    14641 acggcaagag caataaaggc cgggtacagg cttatgatgg ccctatctac attgcggatg
    14701 ccgcgttgtt cgtcaaagcc acgcagccac agttgggcat cagtgatccg tatcaactca
    14761 ccgaagaaca gtatcaggcg gtgctgaaag tgctgcgcga tcaacataat ttgatccatc
    14821 gctactggca tgacactacc gtgcaaatga gcgatttcaa aaacgagggt gtggttgctt
    14881 ccagtgcatg gccctatcag gccaacgccc tgaaagccga aggccagcct gtcgctaccg
    14941 ttttcccgaa ggagggcgtt accggttggg ctgacaccac catgctacat agcgaagcga
    15001 aacatccggt ttgcgcctac aaatggatga actggtcatt aaccccaaaa gtgcagggcg
    15061 atgtggcggc ctggtttggc tcgttaccag tagtgccgca agggtgtaaa gccagtccgt
    15121 tattaggcga gaaaggttgt gaaacaaacg gttttaacta tttcgataaa atcgccttct
    15181 ggaaaacgcc tatagcagaa gggggcaagt ttgttcccta cagtcgctgg acgcaggatt
    15241 acattgccat tatgggcggt cgctaacttc gctggggtgt tttatgacgt acgcagtgga
    15301 gtttgacaac gtctcacggt tgtacggtga cgtgcgcgca gtagatggcg tcagtattgc
    15361 gataaaagat ggtgagttct tctctatgct ggggccgtcc ggctccggca aaaccacttg
    15421 cctgcgcctg attgctggat tcgaacagct ttccggcggg gctatctcta tttttggtac
    15481 gcccgccagc aatctgccac cgtgggagcg ggacgtgaat actgtcttcc aggactacgc
    15541 gctatttccg catatgtcga ttcttgacaa cgtagcctac gggctgatgg tcaaaggcgt
    15601 gaataaaaag cagcggcatg caatggcgca agaggcgctt gagaaagtgg cgttggggtt
    15661 tgtacagcaa cgtaaaccgt cacaactttc tggtggtcag cgccagcggg ttgccatcgc
    15721 cagagcactg gtgaacgaac cgcgtgtgtt gttgctggat gaaccgctcg gcgcgctgga
    15781 tctcaaattg cgagagcaaa tgcagcttga gctgaaaaag ctgcaacagt ctctcggtat
    15841 cacttttatc ttcgttaccc acgatcaggg cgaagcgtta tcgatgtccg atcgggtggc
    15901 ggttttcaat aatggacgca ttgaacaggt cgattccccg cgtgatctct atatgcgccc
    15961 gcgcacgccg tttgttgcgg gattcgttgg gacatcgaat gtttttgatg gactgatggc
    16021 agagaaactt tgtggcatga cgggaagctt cgcactacga ccggaacata tccgcctcaa
    16081 cacccctggc gaaatgcagg ctaatggcac gatccaggcg gtgcagtatc agggcgcggc
    16141 aactcgtttt gaactgacac tgagcggcgg tgaaaaactg ctggtgagtc aggccaatat
    16201 gacaggcgaa gaactgcctg ccacgctcac gcccggacaa caggtgatgg tttcctggtc
    16261 gcgtgatgtg atggtggcgc tggttgagga gaggtgaatg gcgatgaacg tattgcaatc
    16321 accttcacgt ccaggtctgg gtaaggtgtc cggtttcttc tggcgtaatc cggggctggg
    16381 gctgtttttg ctgctgcttg gcccgctaat gtggtttggc attgtctatt tcggctcgct
    16441 gctgacgctg ttatggcagg gcttttatac ctttgacgat ttcaccatgt cggtaacgcc
    16501 ggaactgacg ctggcgaata tccgtgcgct gtttaatccg gcgaattacg acatcattgt
    16561 ccgcacgctg accatggctg tggcagtcac tatcgccagc gccattctgg cttttccaat
    16621 ggcgtggtat atggcgcgct ataccagcgg aaaaatgaaa gcgttttttt atattgcggt
    16681 aatgttgccg atgtgggcga gctacattgt taaagcctat gcctggacgt tattgctggc
    16741 aaaagatggc gtggcgcagt ggtttttaca acatctgggg ctggaaccac tgctgactgc
    16801 gttcctcacg ctaccggcgg tgggaggtaa tacgctgtca acttccgggc tggggcgctt
    16861 tctggtgttt ctctatatct ggttgccgtt catgatcctc ccggtgcagg cggcgcttga
    16921 gcgtttgccg ccgtcattgt tgcaggcgtc ggctgatctt ggcgcacgtc cacgacaaac
    16981 ctttcgctat gtggtgctgc cgctggcaat cccgggtatt gccgctggct ctatctttac
    17041 cttctcactc acgctgggcg attttattgt cccgcagctg gttggccctc caggatattt
    17101 tatcggcaat atggtttatt cccagcaggg ggcgattggc aatatgccga tggcggcggc
    17161 attcaccctg gtgccgattg ttctcatcgc actctatctg gcgtttgtga aacgtctggg
    17221 agcgttcgat gcactctgaa cgcgcaccgt ttttcctcaa actggcggcc tggggcggcg
    17281 tggttttcct acattttccc atcctgatta tcgctgccta tgcgtttaac actgaagatg
    17341 cggcgtttag ttttccaccg caaggcctga cgctgcgctg gtttagcgtg gcagcacagc
    17401 gtagtgatat tcttgatgcc gtgacactgt cacttaaagt ggcggcgctg gcgacattaa
    17461 tcgcgctggt gttagggacg ctggcagctg ccgcgctgtg gcgacgagac tttttcggca
    17521 aaaacgccat ttcgctgtta ctgttgctgc ccattgcgct gccgggcatt gtcactggtc
    17581 tggcgctatt aaccgctttt aaaaccatca atctggagcc gggatttttc accatcgtgg
    17641 tcggtcatgc gactttttgc gtagtggtgg tgtttaacaa tgtcatcgcc cgttttcgcc
    17701 gcacctcgtg gagtctggtt gaggcgtcaa tggatcttgg ggccaatggc tggcaaacct
    17761 tccgctacgt agtgttgccg aatctcagtt cggcgttact ggctggagga atgctggcgt
    17821 ttgccttgtc gttcgatgaa atcatcgtga cgacttttac ggcaggtcat gaacgaacgt
    17881 taccgttgtg gttgctcaat cagcttgggc gaccgcgtga tgtaccggta accaacgtgg
    17941 tggcactgct ggttatgttg gtaacaacct tgccgatcct gggggcctgg tggctaaccc
    18001 gcgaaggcga caatggtcaa taaccactga tacaggaata tgctatgcaa cataagttac
    18061 tgattaacgg agaactggtt agcggcgaag gggaaaaaca gcctgtctat aatccggcaa
    18121 cgggggacgt tttactggag attgccgagg catccgcaga gcaggtcaat gctgctgttc
    18181 gcgcggcaga tgcagcattt gccgaatggg ggcaaaccac gccaaaagcg cgtgcggaat
    18241 gcctgctgaa actggctgat gttatcgaag aaaatggtca ggtttttgcc gaactggagt
    18301 cccgtaattg tggcaaaccg ctgcatagtg cgttcaatga tgaaattccg gcgattgtcg
    18361 atgtctttcg cttttttgcc ggtgcggcgc gctgcctgaa tggtctggcg gcaggtgaat
    18421 atcttgaagg tcatacttcg atgatccgtc gcgatccgtt gggggtcgtg gcttctatcg
    18481 caccgtggaa ttatccgctg atgatggctg cgtggaaact ggctccggcg ctggcggcag
    18541 ggaactgcgt agtgcttaaa ccatcagaaa ttaccccgct gaccgcgttg aagttggcag
    18601 agctggcgaa agatatcttc ccggctggcg tgattaacgt gctgtttggc agaggcaaaa
    18661 cggtgggaga tccgctgacc ggacatccca aagtgcggat ggtgtcgctg acgggttcta
    18721 tcgccactgg ggaacacatc atcagccata ccgcgccgtc cattaagcgt actcatatgg
    18781 aacttggtgg caaagcgcca gtgattgttt ttgatgatgc ggatattgaa gcagtggtcg
    18841 aaggtgtacg tacttttggc tattacaatg ccggacagga ttgtactgcg gcttgtcgga
    18901 tctacgcgca aaaaggcatt tacgatacgc tggtggaaaa actcggtgct gcggtggcaa
    18961 cgttaaaatc gggttcgccg gatgacgagt ctacggagct tggaccttta agctcgctgg
    19021 cgcatctcga acgcgtcagc aaggcggtag aagaggcgaa agcgacaggg cacatcaaag
    19081 tgatcactgg cggtgaaaag cgcaagggta atggctatta ctatgcgccg acgctgctgg
    19141 ctggcgcatt acaggacgat gccatcgtgc aaaaagaggt atttggtccg gtagtgagtg
    19201 ttacgctctt cgacaacgaa gaacaggtgg tgaactgggc gaatgacagt cagtacggac
    19261 ttgcatcttc ggtatggacg aaggatgtgg gcagggcgca tcgcgtcagc gcgcggctgc
    19321 aatatggttg tacctgggtc aatacccatt tcatgctggt aagtgaaatg ccgcacggtg
    19381 ggcagaaact ttctggttac ggcaaggata tgtcacttta tgggctggag gattacactg
    19441 tcgtccgcca cgtcatggtt aaacattaag gataatattg cagatcgtaa gagtatgaga
    19501 tgatcttgcg tactgtcaga gcactgattt ctctgacagt atgtgctgcg tgcaaaatca
    19561 ctacatcaaa taaaccagcc aaatctcata gagataagag taaacaaacc aacagcagca
    19621 aagacattta tcagtactac agttctactg gaaacattca tttttgccac cttttatcat
    19681 tgcccctatg tgtatagtac gggttaagaa aatccgcaat aatactcatt ctgaagatgg
    19741 ataagggcaa gttgctgttt gatgattttt tctcaaactg gtgaaaaaac ttgatgcacg
    19801 tcaaaaaatg acgcatattt gcgcgagttt tattcatctg gctggacgcc cgtacatgtc
    19861 tctctatcaa cacatgcttg ttttttatgc ggttatggca gcaatcgcat ttcttatcac
    19921 ctggtttctt tctcacgata agaaacgcat ccgtttctta agcgcctttc tggtgggggc
    19981 aacatggccg atgagtttcc cggtggcgct gttgttttct ctgttttaaa tcttacgcac
    20041 tggcttgacg gcaccacgca gacttatatc atttggatga atcgataaat ttcacaagtg
    20101 gctaaggaga aagtatgtcg catctggatg aagtcatcgc gcgcgtggat gccgcgattg
    20161 aagagagcgt cattgcccat atgaacgaat tattaatcgc cctgagcgat gacgcggagt
    20221 taagtcggga agatcgctac acccagcagc agcgtttgcg caccgctatt gctcatcacg
    20281 gtcgcaaaca taaagaagat atggaagcgc gccacgaaca gttaaccaaa ggcggtacca
    20341 tcctctgatt aaaatgagcg tctggccacc agccaggcgc caaccacaag taaaattgcg
    20401 ccacaaattg ggccgatcaa cgcccgaagt ggtacgccat ggctgcggaa gatatccagc
    20461 accagaccgc caatcagctg gctggcgacc agtactgcaa tagttgtcgc cgctcccaca
    20521 ttctgatatc cgctgatact ggcgaagaca aaaaacgatc ccagcaagcc aggtataagt
    20581 gtccaccagc gcacgctgga caccagttcg ccaaaccccg ccatgccttg tttaaaccat
    20641 aaaatagaaa caaagagcac aatccccacc agtgagttca gcaacatggc gatgagaatg
    20701 gtagaggagg tctgggtgat gcgcaccatc agcgtgttct ggactaccag accaatcccg
    20761 gctgcaatca aaaaggcaag ggtaagtgac tgattcatcc aatcgcgtcc ggttcagtgc
    20821 gctcgtcgag ttgcaactgc ataaatgtca gatccagcca gcgcccaaat ttagtaccta
    20881 cctgcggcat ttgcgcggtg acgacaaatc ccagcgactg gtgaagatgc agcgaggcct
    20941 gattttgcga ttcgatcccg gcgaccatga catgcttccc gcaatcccgc gcttcatcaa
    21001 tcaatcggct taacaattta cgccccagac ctttcccctg atgatcggga tggacataaa
    21061 ccgaatgttc cacggtatgg cgaaaaccat caaaactacg ccagtcgcca aacgaggcat
    21121 atcccgtcac tacgccatct tcttcactca ccagcaccgg ataacctgct atggtccgcg
    21181 cttcaaacca ggcaatacgg ttatccgcat ccaccgtttg gtcattccag atagccgccg
    21241 tatacaacac ggcgtggtta taaatttccg caattgcagc acagtcggct ttgcgggcaa
    21301 aacggatgga catgtctggc ctcgataaaa taatgatgat gatgttatca atactatcga
    21361 aaacagcccg aagcaacgga ttcctctatg aaattattgc gtgttgcaga ggggatgtga
    21421 cggctgcaaa caaaaccaat cctgtgagcc tgctactatt gatggattca tgccaaaagc
    21481 acaggagagc attatggggc aacaaacgca gcgtaaccga tgttgggttc tggcctcgcg
    21541 tccgcatggc gcgccggttc cttacgtctg gtttcggtaa atgcgctctt tgtcgcacca
    21601 gcgcaagcat ttacagttcc ggaagtggga aaacgtcaat taaatgaaat caatcggatc
    21661 cgctacgaac tggaattaat ggcagttgct ctggctgtcg aaaacctcac tatgcaaaaa
    21721 aacgttgcta tgttgtgcca gcaatacagc cgataaataa aatacgcccg gcaaataccg
    21781 ggcttaaaga taattactca aatcgccacg caatatttat gccgacacca taatttcgtc
    21841 cgggcgcagg ttcgtaatat cgcccgtttg actcattgac aatgacagaa ccaacgtatt
    21901 ctttatcgaa taaattatcg acacgaccaa ataaatccac ggttaaattg tggtaattgt
    21961 atttataccc ggtgaataag ccgacgagtg tataagaagg cgctttggcc gtattttcat
    22021 catctgccat aatatcgccc atataacgag cttccgtgcc tgcataccaa ccatcttccg
    22081 gtatataacc tatcgacgca aaggccatat tacgggcgat ccctggcatc cggttaccgt
    22141 tacaatcctg ttcattgcaa acattgctgc gataggtcgc atcaagccag gtccaggacg
    22201 cttttacgcg aaaatctccc gcgaaacgtt gatcccatgc cagttcagcg ccttgacgac
    22261 gggtctttcc ggcatttttg taagtcgtac gcccaccgct actgctatcg acaacaattt
    22321 catcatcggt gtcggtctgg aacaatgcga gactaagcag cccatcacca atacgcgttt
    22381 tactgccgat ctcaattgta tcgttggtgg agggttttaa accaaagttc ataccgcttt
    22441 gcccatcagc gcgataagac agttcattaa tcgtcggcgt ttcaaaaccg cgcccggctg
    22501 ccagatagac attccaggca tcggtcattg catattttaa cgatccggct ggtagccatt
    22561 tgtgataact ggcatcaccg ctgtcgtcgc cgttacccgg agtaacgtaa tgatcattgg
    22621 aatcaaacca cacggagctg tagcgcacgc cagcatccag cgaaagtttg tcggtcagtt
    22681 gccactgggt ttgtaaatag ggatcgacgt tccacatcaa attgcgttcg tcgcggcgca
    22741 actcaccttt ttgcccgtat tccggcacgc cgcgattcag gcggaagtta ttgtaaccct
    22801 tgcggttttc actcatgttt tcgtagttca ggccggttgt gaacgtgact ggaacgccca
    22861 attcaccacg gtgtgtccag cggctgtcta tcccctggta atggcgttgc agggtaatca
    22921 cgccgcccgc atgtgacggg ttaagttgcg gggccatggg gattgactgg tactgggtcg
    22981 tttctcgctc tccggcatac atcattacac tcatatcatc ttgcgcgctc aggctacgtt
    23041 catagcgcaa cccagcctga gtttgcttga tggtttttcg cgtgtcgtac tgttctgcac
    23101 ggggcgcttg ttgtggattc gccttccatt ctgcttcggt tagcccacct gggtcatctg
    23161 ctttgatatc cacactattg aaaatcaggc ttaatttgct ggcgtcatca atgcgtacgc
    23221 ccagtttggc attggctaaa tttttctgtg cgccgctatg gtcacgatag ccgtgggtcg
    23281 taaaacgtgt ggttgagacg gtgtaatcga catcgccggg ttgtgtgccg tcgcccgttg
    23341 cgcccgtagc tttcagccca tagcgccagc tgccaaaact gccgtagtaa ctactggctt
    23401 caatggttgg tggctgttgt ccggtctggg tggtgacatt cattacaccg ccagacgcgt
    23461 tgccatacag agcagagaag gggccacgca gcacttccac attttgcaca ctgcttaaat
    23521 cgatgttaga tgtttgccct tgtccgtcgg gcatggtagc gggaataccg tccacataca
    23581 ggcgaatacc gcgaattccg taagtggagc gggagccaaa tccgcgaatc gacagctgta
    23641 aatcttgcgc atagttctgc cggttttgta cctgtaaacc aggcactccg gtcagtgatt
    23701 cagacaagtt aatgcgcggt gttgccaggc gcatctcctc gccatccacc acgctcacgg
    23761 ctgctggggt atccagttct gaaaccacct gcggtgcggc actgacaatc atggtctgtt
    23821 catcagcggc aaaaacaacg ggggaaagga caagcagtgc gggcaaaacg gtctgtcgga
    23881 cggaaaaaat cttcatgaaa aaagccaggt taagaatggg aaaacaccgt catggtaatg
    23941 aaattgtaaa tttatggaaa acgaaacggc acaatacgtt aagtaattga gaaaattgta
    24001 gtcgtaacgg taagaaatgc tccacatttg agaaaataat gattaccatt cccatttata
    24061 acaagggcgt aacgatgatt acgcttagcg aagcattgtg aagcagcaaa aatatcggtt
    24121 catcaaaggg agtcgtcatg catttacgtc atctgttttc atcgcgcctg cgtggttcat
    24181 tactgttagg ttcattgctt gttgcttcat cattcagtac gcaggccgca gaagaaatgc
    24241 tgcgtaaagc ggtaggtaaa ggtgcctacg aaatggctta tagccagcaa gaaaacgcgc
    24301 tgtggcttgc cacttcgcaa agccgcaaac tggataaagg cggcgtggtt tatcgtcttg
    24361 atccggttac tctggaagtg acgcaggcga tccataacga tctcaagccg tttggtgcca
    24421 ccatcaataa cacgactcag acgttgtggt ttggtaacac cgtaaatagt gcggtcacgg
    24481 cgatagatgc caaaacgggc gaagtgaaag gtcgtctggt gctggatgat cgtaagcgca
    24541 cggaagaggt acgcccgttg caaccacgtg agctggtagc tgatgatgcc acgaacaccg
    24601 tttacatcag tggtattggt aaagagagcg tgatttgggt cgttgatggc gagaatatca
    24661 aactgaaaac cgccatccag aacaccggta aaatgagtac cggtctggcg ctggatagca
    24721 aaggcaaacg tctttacacc actaacgctg acggcgaatt gattaccatc gacaccgccg
    24781 acaataaaat cctcagccgt aaaaagctgc tggatgacgg caaagagcac ttctttatca
    24841 acatcagcct tgataccacc aatcagcgtg catttatcac cgattctaaa gcggcagaag
    24901 tgttagtggt cgatacccgt aatggcaata ttctggcgaa ggttgcggca ccggaatcac
    24961 tggctgtgct gtttaaccca gcgcgtaatg aagcctacgt gacgcatcgt caggcaggta
    25021 aagtcagtgt gattgacgcg aaaagctata aagtggtgaa aacgttcgat acgccgactc
    25081 atccgaacag cctggcgctg tctgccgatg gcaaaactct gtatgtcagt gtgaaacaaa
    25141 aatccactaa acagcaggaa gctacccagc cggacgatgt gattcgtatt gcgctgtaat
    25201 agatatggcg agggtgaaaa cggagagact tgcttgactt ttatcacgcc gtatccggca
    25261 ccttcgtcag tagtctgaat ttgcccggca cgtagccggg caattttgca attagctcgc
    25321 cgtttcaggc ttaaacacaa tttcctgttt ttcttcatct tcctcgacga ctggcgcagt
    25381 gctgtgaatt tcagcaacgc gtttgcgcac accaaaccaa cctataacca gcagaatacc
    25441 gataaccggc agcgccgcga tggtataagt cccgttcggg taatcgaacg ccatcagcac
    25501 caggacactc agtaaaaaca gtaatgtcag ccaggaagtg aagggcgcgc caggcagttt
    25561 aaaactgaca tccgctgctt tgccttcttt aatcgcttta cgcaggcgca tctggcaaac
    25621 gatgataaac gcccatgaag cgatgattcc cagcgacgcg aagttcaaca caatctcaaa
    25681 tacgcgtgac ggcaccagat agttgaggaa tacgccgacg acatacacaa ccagtgtcgc
    25741 cagaatcccg gcatacggca catgctgacg actcattttc gccataaaac tcggtgcgga
    25801 accgcccatc gccatcgagc gcagaatacg cccggtgcag tacagacctg aattcaggct
    25861 ggagagggca gcggtcaaca ccacaatgtt cataatgctg ccgatgtacg gcacacccag
    25921 tttagagaaa aacgtcacga acggactttg ccccgcctga tacgcgctcc acggcaataa
    25981 cataaccagc aacaccacgg agccgacgta aaacagacca atacgccaaa tcacactgtt
    26041 aatcgcttta ggcaccatgg tctgcggatc tttacattct ccggccgctg tacccaccat
    26101 ttcaatggag gcaaaagcaa acactacgcc ctgaatcaac accagcgcag gcagcaaacc
    26161 gtgggggaaa aagccgccgt tatcggtaat taaatgaaaa ccagtggcgt tgccatccag
    26221 cggctgacca ctaccgagga acactgtacc cacgaccaaa aaggttacaa tggcgaacac
    26281 tttaataagc gcaaaccaga actccatctc cgcaaaccat ttcacaccga tcatattcat
    26341 ggtgccaacg atggtcagtg cagcgagcgc aaagacccac tgcggcacgc cgccaaacgc
    26401 gccccagtaa tgcatataca gagcgacggc ggtaatatca acaatccccg tcatcgccca
    26461 gttgatgaag tacatccagc cagcaacata agcggccttc tcacccaaaa actcacgcgc
    26521 ataagagaca aaactgccac tggaagggcg gtgtaacacc agctcaccca atgcacgcag
    26581 aataaaaaac gaaaacaggc cgcagatcaa atagaccagt gccagtgcgg gccccgccat
    26641 ttgcagtcgc gcccccgcgc ctaaaaacaa acccgtacca atcgcgccgc caatggcgat
    26701 catctgtacc tggcgattgc ccatcgcttt gtgatacccc tcttcgtggg cattaagcca
    26761 gcggcgtttc gcggcgtgtt gatctgaagt gtcggtgttg tgtttactca ttgctctccc
    26821 tgattgcttt aatgaaaaag tcatataagt tgccatgaac aatgtttatt catcctggcg
    26881 atgacaagat tcaccattat tctttatggg tataactgtc ggcgcagaat cctacctttt
    26941 atcatgtaac gatgcaaaat aatcccgcta taaatatgtg gggataaagg cggctggcga
    27001 caaaatagca acctgatgaa attatgacgg aaacaaccac tattggtttg atgatacctg
    27061 actgcagtcc tattcttgcg ccactcgtgc gtcgctctga acgccagtgg ttgcagtgga
    27121 cactggcgtg gttggctcaa tgaa
//
LOCUS       DADSZD010000050        27054 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.050, whole genome shotgun sequence.
ACCESSION   DADSZD010000050 DADSZD010000000
VERSION     DADSZD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.050"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            185..1249
                     /gene="ulaG"
                     /locus_tag="J1Z28_001876"
     CDS             185..1249
                     /gene="ulaG"
                     /locus_tag="J1Z28_001876"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBA7716713.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1357..2112
                     /gene="ulaR"
                     /locus_tag="J1Z28_001877"
     CDS             1357..2112
                     /gene="ulaR"
                     /locus_tag="J1Z28_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBA7716714.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRAEQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2109..2858)
                     /gene="yjfP"
                     /locus_tag="J1Z28_001878"
     CDS             complement(2109..2858)
                     /gene="yjfP"
                     /locus_tag="J1Z28_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA7716715.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTIKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3040..3369
                     /gene="bsmA"
                     /locus_tag="J1Z28_001879"
     CDS             3040..3369
                     /gene="bsmA"
                     /locus_tag="J1Z28_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBA7716716.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3518..3793
                     /gene="yjfN"
                     /locus_tag="J1Z28_001880"
     CDS             3518..3793
                     /gene="yjfN"
                     /locus_tag="J1Z28_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBA7716717.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3910..5535)
                     /locus_tag="J1Z28_001881"
     CDS             complement(3910..5535)
                     /locus_tag="J1Z28_001881"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBA7716718.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMAVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(5619..6782)
                     /locus_tag="J1Z28_001882"
     CDS             complement(5619..6782)
                     /locus_tag="J1Z28_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBA7716719.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRGGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAEQAGQESRFIYIEELGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6785..7423)
                     /locus_tag="J1Z28_001883"
     CDS             complement(6785..7423)
                     /locus_tag="J1Z28_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBA7716720.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MSGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPIVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7433..7831)
                     /locus_tag="J1Z28_001884"
     CDS             complement(7433..7831)
                     /locus_tag="J1Z28_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBA7716721.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7849..8508)
                     /locus_tag="J1Z28_001885"
     CDS             complement(7849..8508)
                     /locus_tag="J1Z28_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HBA7716722.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIALPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKCWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTGDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8558..9256)
                     /locus_tag="J1Z28_001886"
     CDS             complement(8558..9256)
                     /locus_tag="J1Z28_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HBA7716723.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9275..9676)
                     /locus_tag="J1Z28_001887"
     CDS             complement(9275..9676)
                     /locus_tag="J1Z28_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HBA7716724.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKLMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(9803..10534)
                     /gene="rlmB"
                     /locus_tag="J1Z28_001888"
     CDS             complement(9803..10534)
                     /gene="rlmB"
                     /locus_tag="J1Z28_001888"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBA7716725.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(10625..13066)
                     /gene="rnr"
                     /locus_tag="J1Z28_001889"
     CDS             complement(10625..13066)
                     /gene="rnr"
                     /locus_tag="J1Z28_001889"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBA7716726.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKVGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(13105..13530)
                     /gene="nsrR"
                     /locus_tag="J1Z28_001890"
     CDS             complement(13105..13530)
                     /gene="nsrR"
                     /locus_tag="J1Z28_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBA7716727.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(13735..15033)
                     /gene="purA"
                     /locus_tag="J1Z28_001891"
     CDS             complement(13735..15033)
                     /gene="purA"
                     /locus_tag="J1Z28_001891"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBA7716728.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMSGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(15137..15334)
                     /locus_tag="J1Z28_001892"
     CDS             complement(15137..15334)
                     /locus_tag="J1Z28_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBA7716729.1"
                     /translation="MNSTIWLALALVLVLEGLGPMFYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(15416..16420)
                     /gene="hflC"
                     /locus_tag="J1Z28_001893"
     CDS             complement(15416..16420)
                     /gene="hflC"
                     /locus_tag="J1Z28_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBA7716730.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(16423..17682)
                     /gene="hflK"
                     /locus_tag="J1Z28_001894"
     CDS             complement(16423..17682)
                     /gene="hflK"
                     /locus_tag="J1Z28_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBA7716731.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(17768..19048)
                     /gene="hflX"
                     /locus_tag="J1Z28_001895"
     CDS             complement(17768..19048)
                     /gene="hflX"
                     /locus_tag="J1Z28_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBA7716732.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(19124..19432)
                     /gene="hfq"
                     /locus_tag="J1Z28_001896"
     CDS             complement(19124..19432)
                     /gene="hfq"
                     /locus_tag="J1Z28_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBA7716733.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(19518..20468)
                     /gene="miaA"
                     /locus_tag="J1Z28_001897"
     CDS             complement(19518..20468)
                     /gene="miaA"
                     /locus_tag="J1Z28_001897"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBA7716734.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDDVLQVVGAIAG"
     gene            complement(20461..22308)
                     /gene="mutL"
                     /locus_tag="J1Z28_001898"
     CDS             complement(20461..22308)
                     /gene="mutL"
                     /locus_tag="J1Z28_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBA7716735.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGSRPAASWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(22318..23649)
                     /gene="amiB"
                     /locus_tag="J1Z28_001899"
     CDS             complement(22318..23649)
                     /gene="amiB"
                     /locus_tag="J1Z28_001899"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBA7716736.1"
                     /translation="MMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFK
                     TESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTI
                     AIARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSA
                     TGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGH
                     SQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERL
                     LASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(23668..24129)
                     /gene="tsaE"
                     /locus_tag="J1Z28_001900"
     CDS             complement(23668..24129)
                     /gene="tsaE"
                     /locus_tag="J1Z28_001900"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBA7716737.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(24101..25648)
                     /gene="nnr"
                     /locus_tag="J1Z28_001901"
     CDS             complement(24101..25648)
                     /gene="nnr"
                     /locus_tag="J1Z28_001901"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBA7716738.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGRKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCTQRLVQRYGGVAVLKGPGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            25647..26786
                     /gene="queG"
                     /locus_tag="J1Z28_001902"
     CDS             25647..26786
                     /gene="queG"
                     /locus_tag="J1Z28_001902"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBA7716739.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(26769..26822)
                     /locus_tag="J1Z28_001903"
     CDS             complement(26769..26822)
                     /locus_tag="J1Z28_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716740.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         6500 a         7489 c         6630 g         6435 t
ORIGIN      
        1 atttaaattc atttagttaa ttgcattaca cattcaattt aatctttcta cactcaaaat
       61 ccataataac tctcctgcta aaaagtcaaa aaatggtcat tgccttcctg aaaaaatagt
      121 gtcaggatta atcaaaacca atcacatatt gattcgaatt gaactatcac gaaggtaaat
      181 ggcgatgagt aaagtgaaaa gtatcacccg tgagtcctgg atcctgagca ctttcccgga
      241 gtggggtagc tggctgaatg aagaaattga gcaagagcag gtcgcaccag gcactttcgc
      301 aatgtggtgg ctcggctgta ccgggatctg gttgaaatca gaaggcggtg ccaacgtttg
      361 tgttgatttt tggtgcggta ctggcaaaca aagccacggt aacccgttaa tgaaacaggg
      421 tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag ccaaacctgc gcaccacccc
      481 gtttgttctt gatccgtttg cgattcgcca gatcgacgcg gtactggcga ctcacgatca
      541 caacgatcat atcgacgtta acgtcgctgc tgccgtgatg cagaactgtg ctgatgacgt
      601 accgttcatt ggaccgaaaa cctgcgttga tttgtggatt ggctggggcg taccgaaaga
      661 gcgttgcatc gtggtcaaac cgggcgatgt cgtaaaagtg aaagacattg aaattcatgc
      721 acttgatgct ttcgaccgca cggcactgat caccctgcct gccgatcaaa aagcggctgg
      781 cgtactgccg gacggcatgg acgatcgcgc ggtgaactat ctgttcaaaa ccccaggcgg
      841 taccctgtat cacagcggcg attcccacta ctctaactac tatgcaaaac atggtaatga
      901 gcatcagatc gacgtggctt taggttcata cggcgaaaac ccacgcggta ttaccgacaa
      961 aatgaccagt gctgatatcc tgcgtatggg cgaagcgctg aatgcgaaag tagtgatccc
     1021 gttccaccac gatatctggt caaacttcca ggccgatccg caagagatcc gcgtgctgtg
     1081 ggagatgaaa aaagatcgcc tgaagtatgg cttcaagccg tttatctggc aggtcggcgg
     1141 caaatttacc tggccgctgg ataaagacaa cttcgagtac cactatccgc gtggtttcga
     1201 tgattgcttc actattgaac cagatctgcc gttcaagtcg ttcctgtaat ctgtcgcttg
     1261 taccggatag cgtggagatg ctcgcgctat ccggcatagt atttcctggc tatttcaaat
     1321 atcatctaaa aaaatcaaat tttatcggaa tagctcatga ctgaagcaca aagacatcaa
     1381 atcctcctgg aaatgctcgc acaattgggc tttgtgaccg ttgagaaagt cgttgagcgt
     1441 ctgggaattt cgcctgccac tgcacgacgc gatatcaata aacttgacga aagcggcaaa
     1501 ctgaaaaaag tgcgcaatgg cgcagaagct attacccaac agcgcccgcg ctggacgccg
     1561 atgaatctgc atcaggcgca gaatcacgat gaaaaagtac gtattgctaa agcggcctcg
     1621 cagctggtta atccgggcga aagcgtagtc atcaactgcg gctccaccgc gtttctgctt
     1681 gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta attatctacc actggcaaac
     1741 tacctgatcg atcaggagca tgacagcgtg atcattatgg gcggacagta caacaaaagt
     1801 cagtccatca ctttaagccc gcaaggcagc gaaaacagtc tctatgccgg gcactggatg
     1861 tttaccagcg gtaaaggact gacagcagaa gggttgtata aaaccgatat gctgacggca
     1921 atggcagagc agaagatgct gagcgtggta gggaaactgg tggtactggt tgatagcagt
     1981 aagattggcg aacgcgcggg aatgcttttt agccgtgccg aacaaatcga tatgctgatc
     2041 actggcaaaa atgctaaccc ggaaatcctg caacaactgg aagcgcaagg ggtcagcatt
     2101 ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag catctaacgc ctcaggcgta
     2161 atgcggtgac gcacgcctgg ctgccatgaa caggttagca gtttatcccg ccccgtctcg
     2221 cttaaggcct gctgcagacg tagtgattcg tcggcaggca cgacgtcatc gtcaaggcca
     2281 tgccacagca gtagaggtct gtcaccaagt tgttccaggt ggtttgtcgc ttcccactct
     2341 gccagcggcg cgacaatgtt attgaattca ttctgctgtg ctgccgtttc aggtatcagc
     2401 ggtggaaaca gtgaacgggc gagcgatgta aaatagcccg atcccatcat gctggcggta
     2461 cattttatcg tggggtggcg agcggtaatt cccagtgccg tcatcgcgcc catcgacgcg
     2521 ccaccgactg ccagacggtc atcacgcagc cagttttctt cggctattgc cgcacgtaag
     2581 gtagtgaatt cctgcatact ttgtagcaag atttgccaga attgatttaa ccgccgcgct
     2641 gcgtcgccac taaaacggct accgtgatcg ggcgcatccg gcatgatcac ccgcaaacca
     2701 gcctgcgcca gcgcaacggc aaaatagcta tacaccagac tggacgaagt aaagccgtga
     2761 taaaaaatca cacacggtaa cggggtatct ttttgcccga caggataagc atgaagaacg
     2821 ggaatatctg ccagctcgcg tgattctatt tcaatcatct gtctctcctt tttgtctggt
     2881 atgacatgaa gaaatcctta gcacaagttt ctgctggcgg tattagttca taatgttgag
     2941 atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg cgtaccaggt aacaattcgg
     3001 gaacattccc ccaaagttaa attaataagg cactacacta tggttagcag gaaacgtaat
     3061 agcgttattt accggtttgc cagtttatta ttggtgttga tgttaagtgc ctgtagcgca
     3121 ctgcaaggta cgccacagcc agcaccgcca gtaacggacc atccgcaaga gattcgccgc
     3181 gaccagacgc aagggttaca gcgaataggt agcgtaagca cgatggttcg gggttctccg
     3241 gatgacgcat tagcagaaat cagagcgaaa gctgtcgctg caaaagctga ttattacgtt
     3301 gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt attcacaagc cattttgtac
     3361 cgtaaataaa tcagacaact tacatagtca gctttacatt gctttgcgtc cctatgcgtt
     3421 tacctgtgca caataaatta ccggctggaa gctcgggcgt ttattaccgc gatggaatgc
     3481 cctgcaacat gtggggaaac gaaaaatgga gctgacgatg aaacaattac ttgcctcacc
     3541 ctcgctgcaa ttagtgactt atcctgcgag cgccacggca cagtctgcag aattcgccag
     3601 tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc tcagttagca atatttcagg
     3661 ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc gatgaacaag gtgcgtcatg
     3721 gtaccgcatt attaccatgt acgaagacca gcagcctgat aactggcgcg tacaggcgat
     3781 actgtatgcg tgagtgtcat ctacagcaaa tgaacaatgc cggatgcggc gcgagcgcct
     3841 tgtccggcct acaattttaa aatatttcat ttagttaatc gaaaccagcg tcgcatcagt
     3901 cgtatacgct tacaaacaca ctcctcccgt cgcccgcagc aataaatcat tctggatctg
     3961 ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc ttacaccacg cctgcgccat
     4021 tggcggagag gcatatttca acatttgcgc accgcagccc agcaggaata gctgatgagt
     4081 aatctctcgc cccaattctt cagctggctt acgcagctgc tgctgtaaac gacgaaccgc
     4141 gcgatcaaaa tagcgatcct gccctttcac ttccacaaat gcttccgaca ataagtcgta
     4201 tacgcccgct tgcttattga gaacgcgcag cacatccagg cacataatat tgccggaacc
     4261 ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc ggcagctcgc tctcctcgca
     4321 ataaccaatg ccgcccagca ccgccatcgc ttcggccaca aacggaatgc cacgtttgca
     4381 gatcacaaat ttcgccgcag gcgtaaataa acgcgcccac agagcttctt tggcatcggc
     4441 gcgccggtcc cacgctcgcg caagacgaaa cagcaacgcc gtttgccctt caagctgaag
     4501 tgccatgcga cttaagacat gacgcataag aggctgttgg atcaatggat taccaaaaac
     4561 atggcgttga tgtgcatggt aaatcgccag cgaaaatgca cggcgcatca tggcatggct
     4621 acccagggcg caatcaaaac gcgtcatccc acccattttc aggatcagac gaattccttc
     4681 cccttcctgc cccaacaacc agccaatggc atcctgaaac tccacttcgc agctggcgtt
     4741 agagcgatta cccagcttat ctttcagccg ctcgaggcga atcgcgttgc gttgcccgtc
     4801 aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc gcggtctgtg ccagcaccag
     4861 atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc cccaccagcc gataagaacc
     4921 atcgtccaga cgctctgcgc gggtggtgtt gctcatgaca tcggaaccgc cctgcttttc
     4981 cgtcattccc atgccgatca acaagccacg tttttgccca cctggcaata agtgagaatc
     5041 gtagcgatcg ctcagcagcg gcgtggtcca gtcctgaaac ggcgcgggta acatctgtaa
     5101 caacaatggc gtggcggcaa aggtcatcgt tatcggacat aacgacccag cctcaacctg
     5161 cgcatgtaac ataaaacgcg ccgcgcgcgc cacaaatgcg ccggagcgag cgtcttcttc
     5221 ccaggcaaga ttgtgcaccc gattggtaca tagcgcctgc atcagcaggt gccaggcggg
     5281 gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg tagcgcaata attcaggcgg
     5341 attcacattc gccagccgcc ccagttcaag ggattcagcc gttcctaact gctgaccaat
     5401 actggcgaga aaatcgctat cccagctagc accttcacgc gttaccgctt cgcaaagcgc
     5461 gccatcagac aggtataagt tgctgttatt taatggtata ggttgattaa aaacggtgtg
     5521 agtttgccag tgcactgtgt ctccctccat caatggcagt caccattagt atggtcactg
     5581 ccatggattc atgacagaaa gagattgcta aaacattctt atccagcaat gtaatgcgga
     5641 acgaaacgtg aggtgtcttt ggtgatcaac gtgttatctt cacggatccc cattccgcac
     5701 gcttcatcat cgacaatcca gctaccgatg agtgtgtagc tatcgccaaa tcgcggcaga
     5761 ggttgaaacg cctggtagat catcggttca tcggcgtaat caccatcagc gtggtcaacg
     5821 acgttattct gaccgtcgaa aatggtgacg tttccgcctt cgcgcgagta aatcggttta
     5881 cgcacatagc tttcgccagc ggcaatctgc ggtttttcac catcgaacca ggatgcaaga
     5941 aggttaggat gaccagggaa gaagcgccaa agcagcggca ttagcccttt attactcaag
     6001 atacttttcc ataacggctc cacccattgc tcgcgacgct tgcgcagcag cggaccgtta
     6061 tcgtcacgca tcatccactc cagcggatac agcttaaatg cacgctggat gacattatca
     6121 tcaagatcgg tcagtacgcc gccgacgccc aaaccgagct cttcaatgta gataaaccga
     6181 gactcctgcc ctgcctgctc ggcgcagtcc tgcaagtaca gcacggtact cctgtcttcg
     6241 tcggtgtcct gacagcagca aaaataaaac ggttcccgac tgtaaagctc gctaaaacgc
     6301 gaaatcaggc gttcctgaat ggcattgtac tgatcggcat cacgcggaat aatgccgcca
     6361 cgccgggcgt cttccagcca caaccactgg aaataagccg actcatacaa cgaggtcggt
     6421 gtatcagcgt tgtactccag cagcttaacc ggtgcattac cacaccaggc aaaatccata
     6481 cggccataca acgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac
     6541 ggcgggatcg ccagttgcgt caggatctct tcatctttaa ccgcgcgatc caccacctca
     6601 aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctgacgcaga
     6661 gtaaagcggt aagcccgact ctcatcccaa tagatttcat tgtcgatgat atgaaagtca
     6721 aatccgttat cggcggcaat ccggtccaga tcccgccgca caggaacgtt gtgtctcagc
     6781 atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgagaaa
     6841 cggttgacgc ttttttggtg gtaaagccgc ccgaagagta agactctttt ttgccagaac
     6901 cggagcgcca ggagtaatca ccggaagtgt tgcgccagac ggggcgggaa gcaaatgagg
     6961 atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc cggcttaaca
     7021 aaaatccaga aacgatcggg atccaactct gttcaacatt gtcgtagtag caattttcgt
     7081 acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa aacgccgctt
     7141 tggcgttgtt ccagccatga gcacagatat ctgaattatt accatcatca atacaatcct
     7201 gcacagtcgc gtaaaacgtt ccgtcgccgt cgttatcgac atcgccacta tcgccacaac
     7261 ctttcaatac gaaaaaagcg gcaccgctca ttagtgccaa cgttaagtat ttttgtgcgt
     7321 agcgattgcg gcgcggctca aaaggattat gcggtctacc aatgcgactg attgccccat
     7381 gtccgatatt tttgttattt ctggatttgc gttttctggc catgatcgtc cattaccatg
     7441 tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata aacattcctg
     7501 ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagc ctgacgccag
     7561 caaaaacgag taattgaatc accagagcaa tcccgcccca tgcgaaatag tctggaatac
     7621 tcaccgcatt gattgccgca ctggataagg ggatcacgta ccccaacaat gttccgctga
     7681 acgccagtgc cgccgcggtg ttattgtttt tgattaactg ccattcgttg tgcggtgtaa
     7741 ttttggagta gataaacagg aaaataatca ccatcgccac gccaataaaa aaataggcac
     7801 taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt aaccaataat
     7861 atgaagtgat gtcagcggga tatcgacacc cagcgcgcgc gaaaataacc actccggctc
     7921 gccgagatcg ttaaaagatt cctcaccatt taacagcaaa tattcgtaag tatctccggt
     7981 cacctgccgt tggtagccca tgctaaaatt atgcacttcc catttggcat ggttttgatt
     8041 ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc tgttaaaaaa
     8101 tcgctgccag catttttcct gccagtttaa ggtcattgcc cccatcgctt tggcgttgat
     8161 cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga caaagagctt
     8221 gatatcatca atgtcatcaa tatcttcgcc gccagtggta ttgatttgca ggaactcatc
     8281 gcccgaagtg tagtaacgga aaatctggct acccccgccc agatcgatgc aactgacggc
     8341 ggccacggta tattcttcac ccggcagcgc aatcagcaat gcatcttcca gcaaacgaaa
     8401 cgctaacgta tcgagcgtaa aaccactatt gagatgcaga cccaacggac cgcgagcgat
     8461 agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat tttatcctta
     8521 aaatagttgc cagccttgca gtgaggctag caaaaaatta ctcgccctgt tggcgttgca
     8581 gtcttgctaa tacatcctgg gcgctacttt tattgctacc gccaattccg gcttccgcca
     8641 gcttttcatc gagatcgcga ccgtctgcga ctttttccaa ctgtgcggca gcatccaggc
     8701 gagcctgacg ttcggcctga cgcgtttgca ggcgttttaa ggattctgcc gctgtcgaaa
     8761 cgctggaaga tgcgccaaca gtagaggtcg ttacagcctg ttgtgcacgc tgcatggctt
     8821 cagtggcttt aacgacttcc atttgctgct caaactgagc gatacgctgc gctgtcgctg
     8881 taactgcttt ttccacgcca tcacgagaga cttccagatt cgacaacact tgctcttcag
     8941 cggtaatgag attctcaagg cgcgcgattt cttcagcaac ttcgttaatc aacgacggat
     9001 tcacgttctt gcttaacgct tccagcgcac gagcttccag actggctttg cgctcacgta
     9061 aatctttcag cttatcgtga cttaatttca ctcgcgccag cagatcaacg cgagattttc
     9121 cggctttatc gagttcagct ttagcgtcac gaatatgctg ttccagcatg cgcacgcctt
     9181 gggtttcttc aatggactct tccgcctggg agataaacga tttccccagc gtaaataaac
     9241 tttttaaaat tcccataaat acccgtcctt gtaattagtg tgaatattct tctgtaattt
     9301 cagccagatc caatgcgtta tctaccagcg aggttatctc aagcaggata tcttcaagag
     9361 aagatttaag cgataacgca ccgaaaacaa tgtaatactc ttcctgttgc acgctggaga
     9421 tccctaccga tgacaatggc atcagcttct gatttcttaa taagaaggta ttaaattcat
     9481 ctggattgct gatactactt accggacaaa taaaggtttc gatgatcatt tggcgggaag
     9541 taaaaagaat attgatttgc aaatcgccat aatcattcat tttaataatt aatgcgcttt
     9601 cgctatttgt tacatcaata ttttgttcag gtacagtttg cagcgccgtc gccagcgcca
     9661 acggattcca tgtcatatat tattccatat agattaagtt taaatattaa taaaatgaat
     9721 atttgcagta cgtaattatc ttaccagcta tagacaaaaa aaaaccatcc aaatctggat
     9781 ggcttttcat aattcagagg aactagctgc gctgacgaac cgcttcaaat aagcaaattc
     9841 cggttgcaac cgaaacgttc agggaagaaa cacttcctgc cattgggatg ctgatcaact
     9901 catcgcaatg ttcacgggtc aggcgacgca taccttcacc ttccgcgccc atcaccagcg
     9961 ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc cgcctcgcct gccgtaccga
    10021 cgatccagat attctcttcc tgcaacatac gcatggtgcg cgccagatta gtcacccgaa
    10081 tcagcggaac gctttctgcc gcaccacagg ccactttttt cgccgtggcg ttgagctgtg
    10141 cggagcgatc tttcggcaca atcaccgcat gaacaccagc ggcgtccgcg ctacgcaggc
    10201 acgcgccgag gttgtgcgga tcggttacgc catcgaggat cagcaggaac ggttgatcga
    10261 gcgaagcgat cagatccggc agatcgtttt cctgatactg ccgtcctggc ttcacacggg
    10321 cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc gagatattgg cggtttgcca
    10381 gctggataac cacgccctgg gactcaaggg cgtgaatcag cggcaacaga cgtttatctt
    10441 cacggccttt taaaataaag acttcctgaa aacgttcagg ggcgcgctcc agcagggcct
    10501 gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt actcgttgtt tacgttttgc
    10561 cgggagcgcc acgcttgccc ggcctacgga taattcaccc tcttctgttt gaagagggta
    10621 atgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct gcggctattt
    10681 tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgct gctttcggct
    10741 tcggtttttt ctcgccgcgg aaggcgctgt ctggctcaaa gttgaccttt ttgccgacct
    10801 gacgccgctt gccgcctttt ttgcctgcat cgcctttttt cgctttctcg cgcgccgttt
    10861 taccgacgtt gcgcggtgcg cgttcgctgg agatcaggct aaagtcgatt ttgcgctcgt
    10921 ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc cagacgataa gtctggccgc
    10981 tggattcacc catcaggcgt tgccctacct ggtcaaagcg atagtagtca ttgtccagcg
    11041 aagagacatg gaccagacca tcaatgaata agtcgtccag acggacaaag aatccaaagc
    11101 cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc ataaagtcac
    11161 acttcagcca gtcagacaca tcgcgcgttg cttcatcggc acgacgttcc gccatcgaac
    11221 agtgctgacc cagttgcagc atctcttcca tcgaataatg gtagccgccg gtttcagtgg
    11281 tgttgccctg atgcccctgc tctttcgcca gcagatattt aatggcgcgg tgcaacgtca
    11341 ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat gccagaccga
    11401 agtgaccacg gttttccgga tcgtaaatcg cctgtttcat cgagcgcaaa agcatggttt
    11461 gcagcatttc tgcatcagga cgatcggcaa ccgattccag taactctgca tagtcgcgcg
    11521 gttccggctt gttaccgccc ggcagttcca gccccagctc cgccagcact gaacggaaag
    11581 aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt tctttggctt
    11641 tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca attaacttgt
    11701 gggcgtcgtt acgctgagtc tgttcgatac gttcaatacg gcgttcagcg ttgaaaatga
    11761 acttcgcttc ttcgctctca aatgagatcc caccgcgttc ttcacgggct ttatccagca
    11821 ctttatagag gttatgtaac tcttcgagat gcttaaccag cggcgcatac tgctcgcgca
    11881 gatcctgatc gccttgcagt atatgccaga ctttggtgta ggtcagacgc gcgtgagaac
    11941 tcatcaccgc ttcgtagaat ttgtagccag tcaggcggcc tttcgacgaa accgtcatct
    12001 cgcacaccat acacaggcgg tctacctgcg ggttgagcga gcacaggccg ttagagagca
    12061 cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtacca cgattacgcg
    12121 cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcggca atcgcgaccc
    12181 ataaacgcca gccgccgccg cgttttttct cacagtaaac tgcatcgtca aagtcacggg
    12241 cgtcttcgcc atcaatggtg accagcggta aatcgcgcag atcgacacgg cccacttttg
    12301 cttcttccgg cacttcttct ttcagcccgg caacctgttg ctcaacagcc tgcggccaga
    12361 tgtacggaat ttcatgggta cgcagagcga tatcaaccgc catgccggtg cccatattgt
    12421 cgcccagcac ttcgacgatt ttacccaccg ctttggtacg gcgagtcggg cgttgagtca
    12481 gttcaaccac caccacaaag cccatccgcg cgcccatgat ctgatcgggc gggattaaga
    12541 tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct tcggtgaagt
    12601 agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc gcttcacgac
    12661 gacctttacg gtcagcgccc agcggctgag ccagcacctg atcgccatga atgcaggttt
    12721 tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaactcgc agaaagccgt
    12781 agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc ggcagcgcat
    12841 agcactgacg gcgagtgaag accagttgac catcgcgctc catcgcgcgc aggcggcgac
    12901 gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc tcatcacggc
    12961 tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta gggatgggat
    13021 tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg gttcctccgt
    13081 tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa gcggttgatt
    13141 ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac tttgcacggc
    13201 cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact cactgctgca
    13261 attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta ttgcactcgc
    13321 cggtttaccc aagcgaatgc cgccattttt tccacgaaca gcagtcacgt agccggcacg
    13381 actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga cgtcagtcac
    13441 ttcagaaata ctggtcatcc gcccttctgg caatgacgcc atgtagatca gcgcacgtaa
    13501 tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat cggggaaaaa
    13561 tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt tcagttcggg
    13621 gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta accgacacag
    13681 caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc agaattacgc
    13741 gtcgaacggg tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag agatgatatc
    13801 gatcggcaca ccggtcagct cttcaatgcg cttgatgtag ttcagtgccg cctgcggcag
    13861 gccgctacga tctttcacgc cgaaggtgga ttcagaccag cccgacatgg tttcgtaaat
    13921 cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagtcactt cacgaccatc
    13981 cggcatacgg taagccacgc agagtttaac ctctttcagg ccatccagaa cgtccagttt
    14041 agtcaggcag aagccagaca gagagttcag ctgtaccgca cgacgaacgg caacggtgtc
    14101 cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac cctgcttgca
    14161 gaggaactcg ccagtttcat caaacagttc ggtcgggaac ggacctgcac ccacacgagt
    14221 ggagtaagct ttgaggatac ccagaacgta atcaacataa cgcgggccca ggccggaacc
    14281 ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag tgccgtggtc
    14341 gatatccagc agcgtaccct gcgcaccttc aaacatgacg aaatcgccac gctgacgcgc
    14401 ctggtcgagc agatcagaaa cgtcaacaac catagaagtc aggatgtcgg caacagccat
    14461 cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt taaccaactg
    14521 gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt tgtcgaaaag
    14581 gtcgccaaca cgcagaccgc gacgtgccac tttatcttca taagcaggcc cgataccacg
    14641 accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat ccagcgcaac
    14701 gtgataatca aggatcagcg gacatgcttc ggacagcagc agacgctcac gaacggggat
    14761 gccacggtct tccagttctt tcatctcttt catcagcgca gccggagaca gcacaacacc
    14821 gttaccgatg atgctggtta cattctcgcg gagaatacct gatggaataa gatggagaac
    14881 ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct ggtagcgtac
    14941 aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt cgtcacccca
    15001 ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt tgcttaaaaa
    15061 tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa atgacctcga
    15121 tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc acaacgcccg
    15181 caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg gtcatcgcag
    15241 agatcatctt cttccatgcc ttcgggtaaa acatcggccc taaaccttcc agtaccaaaa
    15301 ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga aaaagaaaac
    15361 caccgacatc cctgaggatg tggtggcttt attgacctgt accgcagtcg ttatattaac
    15421 gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctgtccggg ctcatgacca
    15481 tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg atgaatgcgt
    15541 agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttct gcatcgcctt
    15601 caccacgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc acttcatagt
    15661 cggcagttgc acgcagtttt tccgcttctt cctgaccttg tgaacggtga cgacgcgcta
    15721 ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg gtcggcaggt
    15781 tgatctgctt aattcgcaca tcgacaactt caatacccag cgccgccata ctgttcggat
    15841 tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct tcggcaatgg
    15901 cgttatctgc tgcaggggta gtaacttcat cttctgtacc cgcagaaccg gagttcagcg
    15961 cgtcacgtac ttcgagggtc agacgaccac gagaatcggt gacgatgtct ttcacgtcca
    16021 ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc acttccgctt
    16081 gcgaaatgtc gccaccaccc gttgccagat agtaacggct gaaatcgctg atgcgccatt
    16141 tgatgtaaga gtcgacgatc aggtctttct tctctttggt cacaaagcgg tcggcctggt
    16201 tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac ggtatcttga
    16261 aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt accttaccaa
    16321 aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg taaagcacta
    16381 ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc cctgacgctg
    16441 gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata atatcgccct gactggtgga
    16501 cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac gcagcagatt
    16561 gctggcaccg ttatcgctct tcgccgcagg ggcgttacca cctttcagca tctggtctaa
    16621 tggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg tgttgcccaa
    16681 cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg cggctttata
    16741 ttccggcaga agtttagcaa agcgcgccac ttcaccctga gcttccagga tggtctgggc
    16801 cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac gcggctgaac
    16861 ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac gcgcggcaat
    16921 cgcatcgtca aacgccgctt ttacttcttc cggcggacga gcagcctgga agttgacgtc
    16981 cagcagagtg atgcccatgt catacggacg aatcgtctct tccagttcgc gctgagtatc
    17041 gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt taccgataac
    17101 cccgcgcagg gcgctgtcgg tagcctgacg cagactgtca tccgggctgg tcacgctata
    17161 cagatatttt tccggatcgg tgacgcggta ctgcacgttc atctcaacgc gcactacgtt
    17221 ctcgtcagac gtcagcatca caccagaagc tgccagttca cgcacggctt ccacgttcac
    17281 cggtttgact tcgtcaataa acgtcggttt ccagttcaga cccggctcaa ccagatggct
    17341 gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat agaaaccact
    17401 ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacgcgac cgccaagctg
    17461 cgggcgcggg ccttgcgatg aactgccacc accagatccg gtgcctttac cgccgcccag
    17521 accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg gcccttgatc
    17581 gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc ttccccacgg
    17641 gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc tccatatttg
    17701 ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg cttcagacgc
    17761 tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttgcagagg cgacgccagt
    17821 caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc cactcttttt
    17881 ctattgcctg aagctgataa aaacgacttc tcaggcgccc ttcctgcggc ggcagacgca
    17941 atgtatgctg cgccacctcg ccggaaagac gctccgtcaa agcctgaaaa agctgtggta
    18001 tccccgctcc ggtctgcgca gaaagccaga cacggatcgg tttgttctct tcatctctgt
    18061 caatacgcgg ttcgaaatcg tccagcatat cgatcttgtt catcaccagc agggttggga
    18121 tctcgtgagc atcaatctct tcaagaaccg tattcaccgc ttcgatgttt tcttgtacac
    18181 gcacatccgc cgcgtcaatg acgtgcagca gcaatgtggc ttgccgcgtc tcttgtaacg
    18241 tggctttaaa tgccgccacc agatcgtgcg gcaggtggcg aataaaccct acggtatctg
    18301 ccaacacggt ttcaccgaca tccgcaacgt caatacgccg caacgtcggg tcgagggtgg
    18361 caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa agagtagatt
    18421 taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg atacgcgatt
    18481 gccgcccctg ctcacgctgc ttttcaactc tttccaggcg cgactgtatc tgcacgatgc
    18541 gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca cgcaaaccaa
    18601 tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgcgta gccagatggc
    18661 gcaactgcgc caactcaacc tgcaacttac cctcatgggt acgcgcacgt tgggcgaaaa
    18721 tatctaaaat aaggccggtg cggtcgatga cacgacactc gcacaaacgc tccaggttac
    18781 gctcctgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagctccc gtcgctttta
    18841 cggcttccgc aatttcaact gctttacctt cacctacaaa atactttggg tgcggcgctt
    18901 tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc agagattcaa
    18961 actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc agtaccgcct
    19021 gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat cagcggggaa
    19081 cgcaggatcg ctggctcccc gtgtaaaaaa acagcccgaa accttattcg gtttcttcgc
    19141 tgtcctgttg cgcggaagta ttctgcgcgc tgctaccatg atgatagtta ctgctggtac
    19201 cgccaccggc gttgttactg tgatgagaaa ccgggcgaga cgggacaaca gtagaaatcg
    19261 cgtgcttgta aaccatctgg ctgaccgtgt ttttcaacag gatcacgaac tgatcaaaag
    19321 actcgatttg tccttgcagc ttaataccat tcaccaaata aatagaaact ggaacacgtt
    19381 cccgacgcag tgcgttcagg aacggatctt gtaaagattg ccccttagcc attctctctt
    19441 ttccttatat gcttatttgt actttgaatc tttcgattct gaaaaaattg cgcacgatac
    19501 gtctcaattg tacacattca gcctgcgata gcaccaacaa cctgtaatac gtcgtcacgc
    19561 gcctgttctg gtttttcact gtcaagccag tgaacccctt cccaaccacg cagccaggtt
    19621 atctgccgct tcgccaactg tctcgtggcg caaacacctc gataaaccat ttcatcgtat
    19681 gagatttcgc cttcaaggta agaccacatc tggcgataac ccacgcaacg aatggaaggc
    19741 aaatccgtat gcaaatctcc tcgggcaaaa agcgcccgga cttctgcttc aaaacctgaa
    19801 gccaacatct gatgaaaacg ctgctcaatt cgttgatgga gcagttcacg gctcgccggg
    19861 gcgatggcga actgatgcac ctgatacggt agagcgtctc ctgacgtttg cgtcagttcc
    19921 gttaaagttt tacccgaaat gaaaaaaact tccagtgccc gggaaagcct ttgtggatca
    19981 tttggatgaa tccttgctgc cgcaaccgga tcgatctcct gaagttgacg atgcaatgac
    20041 tcccaacctt gctctgccgc ctgttgctca attctggccc gtacttccgg gtctgccgac
    20101 ggtagcggcg acaacccttc cagcaatgcc ttgaaataca acatcgtacc gcccactaat
    20161 agtgggatcc gccccgccgc ggtgatatcg gccatttccg ccagcgcatc gcggcgaaaa
    20221 tcagcagccg agtaagcctg cgacggatcg cgaatattca gcaatcggtg cggcgcggcg
    20281 agtaactctt cagcgttcgg cttcgccgtc ccgatatcca tccctttgta aataagggca
    20341 gaatcaacgc ttatcaactc tactggtaaa attttacgca gctcaatggc taacgccgtt
    20401 ttaccggagg ccgtcggccc catcaaaaaa atcgccttag gcaggctcgc cttactgata
    20461 tcactcatct ttcagggctt ttatcgccgg atgtaaatca acagattgta acagaccacc
    20521 cggcggcgtt ttcacaagtt gcggacataa ccgttccacg tccgccagca gggttatggc
    20581 ctgtgccatt gaccactgcg catgttcgct catcagattt cgtgcaatcc actgcgcaat
    20641 attgccaggt tcgaatacgg actgcttcgc caggtagcct atcagttcag gaatcaagat
    20701 ttgtaaattt tgttggcgta agggtaaagg cacggccctg atggtcacat gctgcgcatc
    20761 ggactggaaa tcaataccca gttccgccag ggcagactgc gctttttcta atgccgattt
    20821 ttcttcgcca gaaactttta gccgcaacgg aataagcagc ggttgggcgc aaactggcac
    20881 ttcacccggc gtcaattgtg cctgacgcag ccaacgttct gccactggca aggataaaag
    20941 tgaaatgttg ccatcgcgct caagcaacgc acagtcggaa tggacgatag tcagtacccg
    21001 accaaaactc tgactgttcg ccgcaagtgc aggttcctgc ggttctggcg cttttggttt
    21061 ttgcatcggc gcgggcgttt gcaaaagctg gcgatacact tcaccttgct gtttctggta
    21121 accaggctgc gcattcggcc aggaggcagc cggacgactg cctgaggcgg gcgcaggaga
    21181 gtagcgcgga gctaccggct cacgaactgc cggttcagca aagtgattgc gcccagccgc
    21241 cacgcgattt tccggaatgg cacgcggtgc aggttgtggt tcatcgtcca gcggtagcgg
    21301 cgtttccagt tgctgttgta gcacgctcag cacgccctga tagataaagt catgcaccag
    21361 acgcgactga tggaaacgca cttcgtgttt ggcagggtgc acgttgacgt ccacctgatg
    21421 cgggtcgatc tccagataca acacaaatgc cggttgctga tcggccccca gtttgtcttc
    21481 gcaggcctgg cggatcgcgt gattgatcag gcgatcgcgc atcatgcgac cgttcacgta
    21541 gcaatactga atttctgcca gtgcgggcgt agtgtgattt ggatcggcca cccagccgcg
    21601 cagcgtgaga tcgccgtgtt gccattcaat cgccagcgct tgttcgagaa aagcggtgcc
    21661 gcaaatcgcg cctaagcgcc gttctttttg cccgccttcc ggcactgcgc ggtactggcg
    21721 tacaattttg ccgttatgcg acaggttgat agtgacgtcg aaacgcgcca gcgcaatgcg
    21781 gcggatgatt tcatcgatat ggttaaattc ggttttctcg gtgcgcagga atttgcgccg
    21841 cgccggggtg ttgtagaaca ggtccagcac ctccagcgtc gttcccaccg gatgcgccgc
    21901 cggtttaacc gtcacgtcca tatcgcgccc ttcggcatag gcctgccagg cttcctgctg
    21961 ttctgcggtg cgtgaagtga gcgtcaggcg ggaaaccgaa ctgatactcg ccagcgcctc
    22021 accgcgaaag cccagactga taatggcttc gagatcgtcc agagaggcga ttttactggt
    22081 ggcatgacgc gccagcgcca gcgccagctc gtcttttttg ataccgcagc cgttatcacg
    22141 aatgcgaata agtttcgccc caccgcgttc gatatcaata tcgatacgcg tcgcacctgc
    22201 atcgaggctg ttttccacca gttctttgac taccgacgca ggtcgctcga ccacctcacc
    22261 tgcggcaatc tggttcgcca gttgtggcgg taagacctga attggcatca atcgtcctta
    22321 gtttggcagc gtgcgatctg gcgtcgtcac cgtactggcc gtttgtgccg ttgcaccctg
    22381 cggcgcagat tgcatcggat gcgcaaggaa gtaattgcgc aggcctttgt aaatggcttc
    22441 agccagctgt tgttggtaat cgtcgctcgc cagcaaacgt tcttcgctgt tgttactgat
    22501 aaaaccggtt tcgaccagta ctgacggaat atccggcgaa cgcagaacgc cgagactcgc
    22561 gtgttctggt cggcgtttat gtatttcgcc aatgcgttgc aactgactga tcatactggt
    22621 cgctacatca taccctaccc gctgggaatg accgaactgt aaatccagca ccgcctggct
    22681 taaatagggg tcagactgac tgttcgccag cacatcgccc gctccgccca gcagctccga
    22741 ctgtttctcg tgctgctcca gccagctggc catttcgctg ttggcgcgac ggttagagag
    22801 cacccatacg gaagcgccag tcgcactgcg gttcggtgct gcatcggcat gaatcgacac
    22861 aaggaaattg gcgttttgct tacgtgccac atcactgcgc cccatcaccg agataaagta
    22921 atccccgtca cgggttaaaa cgcccttaaa catcggatcg ttattgagca aagtacgcaa
    22981 tttacgcgcg atggcgatgg tgacattttt ctcccgcgta ccaccggggc cgatagcgcc
    23041 agggtcctga ccgccgtgtc cggcatcaat agcgatgata attttatcgc cagtgttagc
    23101 cgtcgcgcgc gctgccggac gcgttaccgt attactgctg ataacacccg tagtgcggtt
    23161 actttccgtt ttaaacggat tgcgcgccgg ttcgctgacg cgcggtgcga caaccgcagg
    23221 cgtttcaacg cgtttcgcaa ccattggcgg aggcgatggc ggcgcatcgg cgttaatcgt
    23281 aaagacaaca gtgtaatagc tgccattctg ccgcttcacc gcttcggttt taccgttttc
    23341 ggtgagatcg accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc
    23401 cttcaccaga ttattgccgc taaacaacaa cggcagcccc tgaattaccc ccgtttgttt
    23461 gatatcgagc gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat
    23521 aaaactcaac gttatccgtg cctgttgatt accgttagaa acctgaatat cagagagcgt
    23581 cgcggcaccc accggcgcgc acagcagcag caacgtcgct accaaccaat ttctgatgcg
    23641 atacatcatc ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc
    23701 cgcagaggaa accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat
    23761 ttcgacatcc gggtcaggaa gaacacctgt accttgttgt ggccactcca ccaggcagat
    23821 ggcatcgttg gcaaaataat cgcggatccc cataaactcc agctcctcgg gatcggcaag
    23881 gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt
    23941 ataagtgggg cttttgacat taccctgatg acccagagcc tgtaaaaagc cccggctaaa
    24001 ggtggtttta cctgcaccta aatcgccata cagatagatt acggttgcgc catcgcaggc
    24061 tttcgctacc cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg
    24121 attcatcatg gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga
    24181 gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgccgcac
    24241 cgtgcgcgac acagcccgca caggctgcat cataagggct cattttttgc ccaagcaatg
    24301 cgccaataat accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg
    24361 catcaataat gcctaaagcg tcaggatggg cggcgaccac ggttccggga cctttcagca
    24421 ccgctacgcc gccataacgt tgtaccagac gttgggtgca atgtaagcgg tcactttcaa
    24481 tttcagcgac ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc
    24541 gattgtgacg cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca
    24601 tcggtttgcg aaaattctca actttttgca gggcttttct cccccactct tgctggccca
    24661 gaccgggacc aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatag
    24721 tcagttcatg caccatcaat tccggtcgtg cagtcagcag cggcgcaatg ttctcactgc
    24781 gggtcagtac tcggactaaa ccagcaccag cacgcagcgc cgcttccccc gtcatacgaa
    24841 tagcccccgc cgtgccgtga tcgccaccaa taatcaccag ccgcccgtga tcgcctttat
    24901 gcgaagtcgg gcgacgcggt atcagccact gagaaagttg ttctgccgaa aaccgctgaa
    24961 ttttcgtctc ctgacctgcc agccagctat ccagccccag tgagtcaaaa tgcagttgtc
    25021 cggtaacatc ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg
    25081 tgtgatcggc gttgattacc gcgcctggcg tagcgccggt ttcagccaga aggccggaag
    25141 ggatatcaac cgccacaatc ggcgcagggt gagtattagc gtggtcgatt aactggctaa
    25201 tggattcgcg gggcgcttgc tgcaagccgg tgccgagcag cgcatcaaca atcagatcta
    25261 ccgattcggg ccagacaata ttcgaagcat ggatctcgcc tcccgcgttt aaccatgctt
    25321 cgcgtgccag cgcggcctct tccggcaacg gtttgtcact ttcctgggcc agcagcgtga
    25381 catcaatacc gatcgctttg gccagccgcg cgaccacgta gccatcgccg ccgttattac
    25441 catgaccgca cagcaccagc cagtggcggg cgtcaggata agccgaacga cacacctgga
    25501 atgcggcttc gccagcgcga agcatcagct catagagtgt gagccccagc gcatctgccg
    25561 cctcgcgttc tccgcggcgg atatcgtcgg cgtaccagac tgtgtgtggt atacttacgg
    25621 ggtttttctt cattgtatgg tccgtcatgt cagagcccct cgatctcaat caattagcgc
    25681 aaaatattaa acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg
    25741 atctcagcgc gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa
    25801 tggactggat ggcgcgtcac ggtatgctac gtgcccgccc ccatgagtta ttgcccggta
    25861 cgctgcgcgt gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca
    25921 cgctgaaaaa ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca
    25981 aacttctgcg caatcgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc
    26041 tgaattttag accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag
    26101 caggacttgg ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttcgttct
    26161 tctttttagg cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag
    26221 gatgcggtaa atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat
    26281 ataccgtcga tgctcgccgc tgtatctctt atctcaccat cgaactggaa ggggcgatcc
    26341 cggaagagtt gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta
    26401 tctgcccgtg gaatcgctat tcgcaactca ctacagaaga cgatttcagc ccgcgtaagc
    26461 cgctacacgc accggaactc attgagttat tcgcctggag tgaagagaag tttttaaaag
    26521 tcacggaagg ttcggcgatt cgccgtatag gtcacctgcg ttggctgcgt aatatcgccg
    26581 tagcattagg caatgccccc tgggatgaaa cgattttgac agcgctggaa agtcgtaaag
    26641 gtgagcaccc acttcttgat gagcacatag cgtgggcgat tgcgcagcaa atcgagaggc
    26701 gaaatgcgtg cgtggtcgaa gtgcaattac cgaaaaaaca gcgtctggtt cgggtgattg
    26761 aaaaagggtt accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc
    26821 attgcaattc aagccggaaa aaatcgtcaa gtgatcaata cgacaaaacg aaatcttaat
    26881 ttttaactta tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa
    26941 attaagatta cggcctgagg ctgtggataa ctctgtttac aagagtctgt cagagacatc
    27001 aaaaaccagc aataacgcat cgcgtcgctg tggatatttt attgagagaa gaat
//
LOCUS       DADSZD010000051        26735 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.051, whole genome shotgun sequence.
ACCESSION   DADSZD010000051 DADSZD010000000
VERSION     DADSZD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.051"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(210..1610)
                     /gene="asnS"
                     /locus_tag="J1Z28_001904"
     CDS             complement(210..1610)
                     /gene="asnS"
                     /locus_tag="J1Z28_001904"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBA7716741.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1779..2981)
                     /gene="pncB"
                     /locus_tag="J1Z28_001905"
     CDS             complement(1779..2981)
                     /gene="pncB"
                     /locus_tag="J1Z28_001905"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBA7716742.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLHQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLIFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3247..5859
                     /gene="pepN"
                     /locus_tag="J1Z28_001906"
     CDS             3247..5859
                     /gene="pepN"
                     /locus_tag="J1Z28_001906"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBA7716743.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6054..6821)
                     /gene="ssuB"
                     /locus_tag="J1Z28_001907"
     CDS             complement(6054..6821)
                     /gene="ssuB"
                     /locus_tag="J1Z28_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA7716744.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6818..7609)
                     /gene="ssuC"
                     /locus_tag="J1Z28_001908"
     CDS             complement(6818..7609)
                     /gene="ssuC"
                     /locus_tag="J1Z28_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBA7716745.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7621..8766)
                     /gene="ssuD"
                     /locus_tag="J1Z28_001909"
     CDS             complement(7621..8766)
                     /gene="ssuD"
                     /locus_tag="J1Z28_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBA7716746.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVDFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEHVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRNVAQS"
     gene            complement(8763..9722)
                     /locus_tag="J1Z28_001910"
     CDS             complement(8763..9722)
                     /locus_tag="J1Z28_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7716747.1"
                     /translation="MRKIIKLALAGLLSVSTLAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQTKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLTTFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     LSAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(9715..10290)
                     /gene="ssuE"
                     /locus_tag="J1Z28_001911"
     CDS             complement(9715..10290)
                     /gene="ssuE"
                     /locus_tag="J1Z28_001911"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBA7716748.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYVREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPKFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10527..11537
                     /gene="pyrD"
                     /locus_tag="J1Z28_001912"
     CDS             10527..11537
                     /gene="pyrD"
                     /locus_tag="J1Z28_001912"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBA7716749.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11711..12253
                     /gene="zapC"
                     /locus_tag="J1Z28_001913"
     CDS             11711..12253
                     /gene="zapC"
                     /locus_tag="J1Z28_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBA7716750.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12250..13359)
                     /gene="ycbX"
                     /locus_tag="J1Z28_001914"
     CDS             complement(12250..13359)
                     /gene="ycbX"
                     /locus_tag="J1Z28_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBA7716751.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVVRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            13457..15565
                     /gene="rlmKL"
                     /locus_tag="J1Z28_001915"
     CDS             13457..15565
                     /gene="rlmKL"
                     /locus_tag="J1Z28_001915"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBA7716752.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15577..17484
                     /locus_tag="J1Z28_001916"
     CDS             15577..17484
                     /locus_tag="J1Z28_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7716753.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEELEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17614..18867
                     /gene="pqiA"
                     /locus_tag="J1Z28_001917"
     CDS             17614..18867
                     /gene="pqiA"
                     /locus_tag="J1Z28_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBA7716754.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18872..20512
                     /gene="pqiB"
                     /locus_tag="J1Z28_001918"
     CDS             18872..20512
                     /gene="pqiB"
                     /locus_tag="J1Z28_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBA7716755.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            20509..21072
                     /gene="pqiC"
                     /locus_tag="J1Z28_001919"
     CDS             20509..21072
                     /gene="pqiC"
                     /locus_tag="J1Z28_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBA7716756.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21328..21495
                     /gene="rmf"
                     /locus_tag="J1Z28_001920"
     CDS             21328..21495
                     /gene="rmf"
                     /locus_tag="J1Z28_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBA7716757.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21565..22083)
                     /gene="fabA"
                     /locus_tag="J1Z28_001921"
     CDS             complement(21565..22083)
                     /gene="fabA"
                     /locus_tag="J1Z28_001921"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBA7716758.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(22152..23912)
                     /locus_tag="J1Z28_001922"
     CDS             complement(22152..23912)
                     /locus_tag="J1Z28_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBA7716759.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENAPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARMLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24098..24550
                     /gene="matP"
                     /locus_tag="J1Z28_001923"
     CDS             24098..24550
                     /gene="matP"
                     /locus_tag="J1Z28_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBA7716760.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24628..25668)
                     /gene="ompA"
                     /locus_tag="J1Z28_001924"
     CDS             complement(24628..25668)
                     /gene="ompA"
                     /locus_tag="J1Z28_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBA7716761.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEVAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(26024..26533)
                     /gene="sulA"
                     /locus_tag="J1Z28_001925"
     CDS             complement(26024..26533)
                     /gene="sulA"
                     /locus_tag="J1Z28_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBA7716762.1"
                     /translation="MYTSGYAHRSSSFSSAESKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTAEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
BASE COUNT         6708 a         6714 c         6974 g         6339 t
ORIGIN      
        1 gtatgaaaat tgtgctatca gctaagaaaa aactaatccg caataagaat gcagattatt
       61 ttttgtgcgt aaatgttgca gcgtaatatt ccgtaacagg atgatcgtta tggcggggtt
      121 gcaaatagat tgcttgactt aacaaacaga taacttgaca gaaaggataa aaaaagagcc
      181 agcgggcgct ggctcttgaa gacgctaaat tagaagctgg cgttacgcgg agtacgtggg
      241 aacggaataa catcacgtac gttttgcacg ccagttacgt aagcaatcag gcgttcaaaa
      301 ccaagaccga aacctgaatg cggaacagta ccgtagcgac gcagatcgcg ataccaccag
      361 taatcttctt tgttcaggcc catttcaagc atacgctcgt ccagcacgtc cagacgttct
      421 tcacgctggg agccaccaat gatctcaccg atgcccggag ccagaacgtc catagccgca
      481 acggttttac cgtcttcgtt aaggcgcata tagaacgctt taatatcttt cggatagttt
      541 ttaaccacta ccggtgcttt aaagtgttct tccgccagat aacgctcatg ctcagaagag
      601 agatcgacgc cccagtaaac cgggttttca aacttcctgc cgcagttttc gagaatggtc
      661 actgcgtcgg tgtaatccac ctgcgcgaaa tcggcctcaa tgaagcgttc cagacgtgaa
      721 acggcatctt tatctacgcg ttcagcgaag aatttcatgt cgtcagcgcg ttcttcgaga
      781 accgctttga agacatattt cagcatggct tcagccagac ccgcgatatc gttcaggtta
      841 gcaaacgcca cttctggctc cagcatccag aattccgcca ggtgacggct ggtgttggag
      901 ttttcagcac ggaaagtcgg gccgaaggta taaattttgg ataatgcgca agcgtaggtt
      961 tcgccgttca actggccaga tacggtcagg aaagactctt taccaaagaa gtctttgtcg
     1021 aaatctactt tgccctgatc gttacgcggc aggttttcca gatccagcgt tgaaacacgg
     1081 aacatttcgc cagcaccttc ggtatcagat gcggtaatca gtggcgttga aacccagaag
     1141 aatccctgct cgttaaagaa gcgatgcagc gcctgcgcca gcgtatggcg aacgcgcgcg
     1201 acggcaccaa tcaggtttgt gcgcggacgc aggtgagcga cttcacgcag atactcaatg
     1261 ctgtggcgtt ttgccgccat cgggtaagtg tctggatctt caacccaacc agcaacttca
     1321 accttgctgg cctgaatttc aaattgttgc ccctggcccg gcgacgccac gactttaccc
     1381 gtcacaatga ccgagcagcc ggtggtcaga cgcaagacgt cttcattgta attgggcaga
     1441 gaattattga tgacagcctg tacaggatca aagcaggaac cgtcataaac ggcgaggaag
     1501 gagatgccag cttttgaatc tcggcgggta cgtacccatc cgcgcacggt gacttcgctg
     1561 tcaacggcta cgcggccctg gagtacgtcg gctacaggca caacgctcat aatattctct
     1621 ctgttaatag tcggaaaaaa taaacacttg tccacccgta aatgggggga tacctatgtt
     1681 acctggcatc tgcaatcaga caagcagaat tcgcaaatgc agcgaaagat ttcggaatta
     1741 aaggtaaaaa aagggagccg attagctccc tgatgatatt aactggcttt tttaatatgc
     1801 ggaaggtcga acgctttgcg cagcgcccga acaaacgctt tatcgtggca gatagttttg
     1861 ccagggctgt cagaaagttt cgccaccggt ttaccgttac actctaccaa cttaatgaca
     1921 atattcagag gttttacctg ggggatatcg caggtcaggc gagtcccaat accaaaactt
     1981 aattgcacgc gggaagagaa gtggcggtat agctcaaccg ctttgcgtaa atccagattg
     2041 tcagagaaaa tcagcgtttt actctgtgga tcaattccca gcttttcata atgtgcaatg
     2101 gctttttcac cccattcaac cgggtcacca gagtcatggc gcaggccctg atagcgactg
     2161 gcgaactcga caccgaaatc acgcaggaaa gcatccatag tgatgcagtc ggttaatgca
     2221 atgccaagtt ggtcgggata ctcttccagc caggcagcaa gtgcagctcg ctggctgttg
     2281 gctagatccg ggctgatttg ctgatgcgcc tggaaccatt cgtgtgcctg tgttcccatc
     2341 ggcgtgaggg aaagccgacg cgccagatcg tagttgctgg tgcccacgaa ccaggattcc
     2401 tggtgcagac gtttcacgat ggtttcttgt acttcgcgag aaaaacggcg gcgggtgcca
     2461 aaatccatca gatggaagcg cgacatatca agaccggcgg ttaatgccga gaagtcgact
     2521 aatttgtttt ccagcgtgtc gagggcttgc gcaacgtcgg cctgcggtga gcgatagcga
     2581 tgtaccattt cactgataac cgccagcaaa ggaacctccc agaggatgac ttcacgccac
     2641 gggccgctta aacgaatatc cagcttgcca ttatcgttgg acacggtgac ttgttccggg
     2701 ttaaagcgga actcgcgtag ccagttaaga tagtcggcct taaagaaagg cagggcagag
     2761 agccactgat attcatcatc ctgcaggcgc aggtgctgca tcgcctgaac ctgttcacga
     2821 atagcatcgg cataaatacc cagcagatcg tcacctcggc aacgaaactc cgccgcgaca
     2881 tgcacatcgt aatagtgatg aaacacggct tgctgcatat gcaacttata agcatctgta
     2941 tccagcaacg agtgcagaac aggagaagcg aattgtgtca taggtgcgca gtagcgtcct
     3001 cgtacaggag cgtttagtac aataaacatc ttcaggaact gctggagtat accttgtttc
     3061 gcaatttatt gaaccccgat cacaccatat gccacctttc tggtcgatgg cataacgcgg
     3121 tgtatgttat aaaaatgtag caataaaggc gtttgtacct gaaaagatga agattctgca
     3181 tagcgcgatt tacgcaacag gaatagactg aacaccagac tctataaaag atgctaaagg
     3241 ttatttatga ctcaacagcc acaagccaaa taccgtcacg attatcgtgc gccggattac
     3301 cagattactg atattgactt gacctttgac ctggacgcgc aaaagacggt cgttaccgcg
     3361 gtcagccagg ctgtccgtca tggtgcatca gatgttccgc ttcgtctcaa cggcgaagac
     3421 ctcaaactgg tttctgttta tattaatgat gagccgtgga ccgcctggaa agaagaagag
     3481 ggcgcactgg tcatcagtaa tttgccggag cgttttacgc ttaagatcgt taatgaaatt
     3541 agcccggcgg cgaatactgc gctggaaggg ctttatcagt caggcgatgc gctttgcacc
     3601 cagtgtgaag ccgaaggttt ccgccatatt acgtattatc tcgaccgccc ggatgtgctg
     3661 gcgcgtttta ccaccaaaat tattgccgat aaaaccaaat atcccttcct gctttccaat
     3721 ggtaaccgcg ttgcgcaagg tgaactggaa aatggacgtc attgggtaca gtggcaggac
     3781 ccgttcccga aaccgtgtta cctgtttgcg ttggtggctg gtgattttga tgtactgcgc
     3841 gataccttca ctacccgctc tggccgtgaa gtagcgctgg agctgtacgt cgatcgtggc
     3901 aaccttgatc gcgcgccgtg ggcgatgacc tcgctgaaaa actccatgaa atgggatgaa
     3961 gaacgcttcg gcctggagta tgacctcgac atctatatga tcgtcgcggt ggatttcttc
     4021 aatatgggcg caatggagaa taaggggctg aatatcttta actccaaata tgtgctggcc
     4081 cgcaccgaca ccgccaccga caaagattac ctcgatattg aacgcgttat cggccatgaa
     4141 tatttccata actggaccgg caaccgtgtc acctgccgcg actggttcca gctcagtctg
     4201 aaagaaggtt taaccgtctt ccgcgatcag gaattcagct ctgaccttgg ttcccgcgcg
     4261 gtaaaccgta tcaataatgt gcgcaccatg cgcggattgc agtttgcaga agacgccagc
     4321 ccgatggcgc acccaattcg cccggatatg gtcattgaaa tgaacaactt ctacaccctg
     4381 accgtttacg agaagggcgc ggaagtgatt cgtatgatcc acactctgct tggcgaagaa
     4441 aatttccaga aagggatgca gctctatttc gagcgtcatg atggtagcgc agcgacctgt
     4501 gacgattttg tgcaggcgat ggaagatgcg tcgaatgtcg atctttccca tttccgccgt
     4561 tggtacagcc agtccggcac gccggttgtg accgtcaaag acgactacaa tccggaaact
     4621 gagcagtaca ccctgaccat cagccagcgc acgccagcta caccggatca ggcagaaaaa
     4681 caaccactgc atattccgtt tgccatcgaa ctgtatgaca acgaaggcaa agtgatcccg
     4741 ttgcagaaag gcggtcatcc ggtgaattcc gtgctgaacg tcacccaggc ggaacagacc
     4801 tttgtttttg ataatgtcta cttccagccg gtgcctgcgc tgctgtgcga attctctgcg
     4861 ccagtgaaac tggaatataa gtggagcgat cagcaactga ccttcctgat gcgtcatgcg
     4921 cgtaatgatt tctctcgctg ggacgcggcg caaagtttgc tggcaaccta catcaagctg
     4981 aacgtcgccc gtcatcagca agggcagccg ttgtctctgc cggtgcatgt ggctgacgct
     5041 ttccgcgcgg tgctgctcga tgagaagatt gatccggcgc tggcggcaga aatcctgacg
     5101 ctgccttccg tcaatgaaat ggcagaactg ttcgatatca tcgacccgat tgctattgcc
     5161 gaagtacgcg aagcactcac tcgtactctg gcgactgaac tggcagatga gctgctggct
     5221 atttacaacg cgaattacca gagcgagtac cgtgttgagc atgaagatat tgcaaaacgc
     5281 actctgcgta atgcctgcct gcgcttcctc gcttttggtg aaacgcatct ggctgacgtg
     5341 ctggtgagca aacagttcca cgaagcgaac aatatgaccg atgcgctggc ggcgctttct
     5401 gcggcggttg ctgcacaact gccatgccgt gacgcgctga tgcaggagta cgacgacaag
     5461 tggcatcagg atggtctggt gatggataaa tggtttattc tgcaagccac cagcccggcg
     5521 gcgaatgtgc tggagacggt acgtggtctg ttgcagcatc gctcatttac catgagcaac
     5581 ccgaaccgca ttcgttcatt gattggcgca tttgcgggca gcaacccggc agcgttccat
     5641 gccgaagatg gcagcggtta tcagttcctg gtggaaatgc ttaccgacct caacagccgt
     5701 aacccgcagg tggcttcacg cctgattgag ccgctgattc gcctgaaacg ttacgatgcc
     5761 aaacgtcagg agaaaatgcg cgcggcgctg gaacagttga aagggctgga aaatctctct
     5821 ggcgatctgt acgagaagat caccaaagca ctggcttgat acttttgagc caaaatgctt
     5881 gacgcgtctt atcaggccta caagtccgtt gaaattgctg gttttgtagg ctggataagg
     5941 cgttcacgcc gcatcaggca gtcgtgccca actgcctgat gcgacgcttg acgcgtctta
     6001 tcaggcctac aatcccgttg aaaatgctgg ttttgtgcgc cacatccggc atattaacca
     6061 tgacgtcgaa tcggttgttc agagtgccca cgttgcatca cccgctgcaa cacttccgct
     6121 tccagctctg ctaatcgtac cgaccctaac cggcgcggac gggggatatc caccgtcaaa
     6181 tccagaccaa tttttccctc ttcaattaac aacacccggt cagccatcgc caccgcttcg
     6241 ctcacatcat gcgtcaccag caagacggta aagccgtgct cctgccagag tgacacaatc
     6301 aaatcctgca tctcgagtcg cgttaaagcg tccagcgcac cgagtggttc atcaagcaac
     6361 aataaacccg gtcgatgaat cagcgccctt gccagcgcca cacgttgttt ctgcccgcca
     6421 gaaagtgcgg caggccattc ccccgcgcga ttctccagcc ctaccgcagc cagagcctga
     6481 cgcgccgcat cgcgccactg gcctttaagg cctaacccaa cgttatcaat caccgatttc
     6541 cacggcagca gacgcgcgtc ctgaaacatc attcgcgtat catcctgaat ctcagccagc
     6601 ggtgtggtgc ccgctaacac gtcgcctgcg gttggcgttt ccagacctgc cagcaggcgc
     6661 agcagggtac ttttgccacc gccgctgcgg cccaccaccg ccacaaactg acctgccgga
     6721 atatgtaaat ccagttggtt caggacgata ttttccgcgt aatgtttgct tactgcattg
     6781 agcaacaatg gtgtgccctg gttcagacga gcagtattca taccgtggcc tccttcaaat
     6841 gataagccgg gttccagcgc aaccagagac gttctaacag ctgcgcgctg acgtcagcaa
     6901 gtttgccgag cagggcgtaa agaataatag cgactaccac cacgtccgtt tgcagaaact
     6961 cccgcgcatt catcgccaga taaccaatgc cggaattggc agaaatagtt tcggcaacga
     7021 tcagcgtcag ccacatcagg cccaacgcaa aacgcacacc gaccataatt gagggcaggg
     7081 caccaggcag gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgtgcca
     7141 tctccaccag cccacggtcg atattacgga tgccatgcca ggtgttgata taaatgggga
     7201 atagcgtacc gagcgccacc agaaagattt ttgcggattc atcaatgcca aaccacaaaa
     7261 tcaccagcgg gatcagcgcc agatgcggca cgttgcgcaa catctgaatt gaggtatcca
     7321 gcagtcgctc accccagcgt gacagcccgc taatcagccc cagaattaat cccagcgatc
     7381 cgccaattga aaagccaatc aacgcccgcc aggagctgat cgccagatgc tgccacaatt
     7441 cgccgctggc agagagcgtc cagaacgcca tcaccactcc ttccggtgag ggcaaaatac
     7501 gcgtcgatag ccagccaacc gaggaggcca gttgccacac cgccacgatg cccaccggta
     7561 aaaaccaggg agcaactcgc aataaccact tcttcactgg cgttgccatc atcgtgttcc
     7621 ttagctttgc gcgacgttac gggggataaa atcattcgcc accgcttcgc cttgtggatt
     7681 cagcggttgt ggctggggaa tttccgggat ggcgacatcc agatgtggga acagtaactc
     7741 gccgacccga tacgcttctt ccagatgcgg gtagcccgaa agcacaaaac tgtcgatgcc
     7801 aagtgcagcg tattcgttga ttcgcgcggc gaccgtagga ccatcgccca ccagcgccgt
     7861 cccggcaccg ccgcgcacta agccaacacc cgcccataaa ttggggctga tctccagatt
     7921 atcgcgcttg ccgttatgta gcgccgccat tcgctgttgt cctacggaat ccgttcgggc
     7981 aaacgccgcc tgtgctttgg cgatagtttc atcatcaaga tgcgagatta accgctcggc
     8041 ggcctgccac gcttcgtcgt tggtttcacg aacaatcaca tgcagacgaa taccgaaacg
     8101 aattttgcgt ccatgcgcgg cagctttcgc ccgcacgtgt tcgattttct cttttaccag
     8161 ttccggtggt tcgccccagg taaggtagag atcaacttgt tctgccgcca gctcctgggc
     8221 gacatctgac gatccgccaa agtaaagcgg aggataaggc tgttgaatcg gcgggaagag
     8281 cagtttggct ccgcgtacat gaatatgttt gccgttgaaa tccactgtct cgccaagcaa
     8341 caaccgccgc cagacctggg taaattctgc cgaggcttcg taacgctcgc tatgatcaag
     8401 gaacacgccg tcgcctgcca gttcttgggg atcgctgcct gtgaccaggt taaacaacgc
     8461 acgtccgtta gagagacggt caagcgtggc ggcctgccgg gcggcaacgg taggtgaggt
     8521 tacgctgggg cgcagggcga caagaaactt cagccgttgt gtcacaggga tcatcgacgc
     8581 cgccaccagc cacgcatctt cacacgatcg ccccgtcggg atcagcacac cggtatagcc
     8641 cagacgatcc gccgcttgcg caatttgttg cagataaccg tgatcaaccg gacgtgaacc
     8701 ttcttccgtt cccagataat gcccgtcacc gtgggtcggt aaaaaccaga acatattcag
     8761 actcataatt gttttccttc cagttgagtg ggttgccaga tgcgctggcg aatatcgact
     8821 tttttcggca ccagacggtt ttcataaaac agatctgccg tttgctgctg taaggcggca
     8881 acttcagcgc tcaacggttt gatggtggtg ggaggacgat gatccagata cgaggcaatc
     8941 accggggccg gtaagcccat cgtttttgcc agtaaagcga tgctttgttc gcgctggctg
     9001 cgggttaacg catcggcctc actaaaggtt gtcagcacac cctgaataaa agcgccgttt
     9061 ttttctgcat acggacgagc tgccagataa aacgatccag tttgattgag atcggtgccg
     9121 tctttcagca cccgcacgcc gccctgtaat aatgcagcgg agtagtaggg atcccagata
     9181 gcccaggcgt cgacgttacc ttgctggaac gcggcgcggg catcggcagg cgtcaggtaa
     9241 gtgggctgaa tatccgtgaa tttaagcccg gcctggcgaa gggctcgcag taaaagattg
     9301 tgcgaactgg aacctttctg aaaggcaact ttgtgacctt taagatcggc tacggttttg
     9361 atcgggctgt tttctgccac cagaatcact tcggctttgg gcttcggtgg ctcgacgccc
     9421 acgtatacca aatcagcccc ggcagcctgg gcaaagattg gcggaatatc tccggtactg
     9481 ccgagatcga tactgccaac gtttaacgcc tccagcatct gtgggccagc ggggaactcc
     9541 acccaggaga tttttgtttg cggatagcgt ttttccagta actggtggct ttttgccagc
     9601 accataccaa tactgccttt ttgatagcct atacgtaacg cctctggcga agactccgcg
     9661 gcaacagcaa gtgtagagac gctaagtaaa cccgccagcg ccagtttaat gatcttacgc
     9721 atgggcatta cctcgcagag acagaaggtc aggaacctga acatcgcggc ggtgcaacgc
     9781 ctgccagaaa gtttctagcg cggtatcaag acgagtttgc agatttggcg tgaacttggg
     9841 tttgtgttgg taatcaatga cttgcgagtc atcggcaaac acgccgtgca ggatctcctg
     9901 ggctttcagc gtacttaaaa ctggcttaag ggcataatcg accgccagca gatgggccac
     9961 ggtgccgcct gtcgccagcg gcagcaccac tttgccttgc aacgcacgtt ctggcagcag
    10021 gtcgagcagg gttttcagtg caccggaata ggcggcttta tacacaggcg tggcgacaat
    10081 cagcccatcg gcctgttgca gctgttcggt gaaggtcttg agtgccggac tatcgaaacg
    10141 agcataaagc agatcttccg ggacgaagtt ttgcaaattc cagtgataaa cctctacatc
    10201 caggccattt agtttttccc gcacatattc cagcaaggag ctggagcgag aaggaaagcg
    10261 aggactaccc gccagggtga tgacacgcat actctctcct tataaccaat tgttcttgtt
    10321 ttgttaacat tgataacaat tcggtcagtc tgacagaggg cgtccgccag tcgaaatgat
    10381 ttatctgagg aagaaaagcc aaaaaaagca taaaaaaagc gcaataagaa aacgtttgct
    10441 atttattgcc gcaggtcaat tcccttttgg tccgaactcg cacataatac gcccccggtt
    10501 tgcacaccgg gaatccagga gagttcatgt actacccctt cgttcgtaaa gcccttttcc
    10561 agctcgatcc agagcgcgct catgagttta cttttcagca attacgccgt attacaggaa
    10621 cgccgtttga agcactggtg cggcagaaag tgcctgcgaa acctgttaac tgcatgggcc
    10681 tgacgtttaa aaatccgctt ggtctggcag ccggtcttga taaagacggg gagtgcattg
    10741 acgcgttagg cgcaatggga tttggatcga tcgagatcgg taccgtcacg ccacgtcccc
    10801 agccaggtaa tgacaagccg cgtctctttc gtctggtaga tgccgaaggt ttgatcaacc
    10861 gtatgggctt taataatctt ggcgttgata acctcgtaga gaacgtaaaa aaagcccatt
    10921 atgacggcgt tctgggtatt aacatcggca aaaataaaga tacgccggtg gagcagggta
    10981 aagatgacta tctgatttgt atggaaaaaa tctatgccta tgcgggatat atcgccatca
    11041 atatttcatc gccaaatacc ccaggattac gcacactgca atatggtgaa gcgctggatg
    11101 atctcttaac tgcgattaaa aataaacaaa atgatttgca agtgatgcac cataaatatg
    11161 tgccgatcgc agtgaagatc gcgccggatc tttctgaaga agaattgatc caggttgccg
    11221 atagtttagt tcgccataat attgatggcg ttattgcaac caataccaca ctcgatcgtt
    11281 ctctggttca gggaatgaaa aattgtgatc aaaccggtgg cttaagtggt cgtccgcttc
    11341 agttaaaaag caccgaaatt attcgccgct tgtcacagga attaaacgga cgcttgccga
    11401 ttattggtgt tggcggcatt gactcggtta tcgctgcgcg tgaaaagatt gctgcgggtg
    11461 cctcactggt gcaaatttat tctggtttta tttttaaagg tccgccgctg attaaagaaa
    11521 tcgttaccca tatctaatta tttcttcaaa ttcctcttta taagataatc agggctttat
    11581 tttcagccct ggttgtttta tattcatcgc tgttgcttat ttagacattt tgtactttta
    11641 ttgttgaggt tattaagcga agcgacaatg gattgtgtcg ctgcggtttt ataaggttgg
    11701 ggagtggcag atgcgaatta aaccagacga taactggcgt tggtattacg atgaagagca
    11761 cgatcgaatg atgctcgatt tagccaatgg tatgctattt cgctcacgtt ttgcgcgcaa
    11821 aatgttgact ccagatgcct tttctcccgc aggtttttgc gttgatgacg ccgcgctcta
    11881 tttctctttt gaagaaaagt gccgcgattt taatttatct aaagaacaaa aagccgaact
    11941 ggtgctgaat gcactggtag caattcgtta tctcaaaccg caaatgccga aaagctggca
    12001 ttttgtatct catggtgaaa tgtggatgcc catgccgggc gatgcggcct acgtttggtt
    12061 gagcgacact catgagcaag tgaatttact ggtcgttgaa tctggcgaga atgccgcgct
    12121 atgtctactg gcacaaccct gcgttgttat tgcgggtcgc gcgatgcagt tgggcgacgc
    12181 cattaaaatc atgaacgaca ggctgaaacc gcaggttaat gttgacagct tcagcctcga
    12241 acaggcagtc taacgcgcca gcttaagcgc agtcttcggt acacaactac agcaaagaat
    12301 agtgccatca tcgcctattg ctgatttttt tagcggcgtg acttcgcctt ctaaaagctg
    12361 aacacggcaa cttccacaaa tgcccgcgcg gcaagaataa gggatacgaa ttccctgatt
    12421 ttctaattgc tccagcagca cctgttggtt atttccacga aatgcctgtc cctgccagtc
    12481 aatatctacg tttgcgtccg gttgttgcgt gatgttggca gtatcatcag cggcacctgc
    12541 gccgtaaatt ttagccggag ccgttgccag aatttccacc tcgtcgccaa cgcgaatcac
    12601 gccgctatta cgggcaatta aattctgacc aaaatcgaca tcgccgttat cctgggcagt
    12661 acggaaagat tgcagtgttt ttaatggttc gcctgccgga tgtttttgcc ctttttctgg
    12721 gctgacggtg gtgaaaatac agcggctaca aggcttaacc acatcaaaca cgacatcacc
    12781 aatgcgaacc actttccagc tatcttcttc ccaggccgat gcaccggaaa ccaccagatt
    12841 ggggcggaat tgctccattt ttacactggc cggacaacgt tgttggagat cacgtaacga
    12901 ggcctcgtta gcaagaaggt aaggatagcc atcagcaaat gacaggggta cagtgttgtg
    12961 gcgtttcacg cgccgggtca tttgtggccc cacccagcgt aattgcactt cgcgggagaa
    13021 aaatccactt agccatttgt tgatcgcgtc tggcgcaatt cgcgcggtaa aatgtgtgcc
    13081 ccaaacttcg gttggtgcgt cttgtgtggc gaaatcagca aaacgaacat atgcactact
    13141 gccatctggt gcggtgagat gcaagccatc atgcacgggt gaaggagtaa accgtaccat
    13201 tagcggaaac tggcgagcgg taataaacgt accgtcaggt tccgtgatca taaagatgcg
    13261 atcgaaggcc agaccactga catctgccag ggtatgtgta agaccaatgc cgcgcatcga
    13321 tttaacagga tgaataaaaa gccgggttaa tgtcgccacg gtgcggtcct caaatgaaaa
    13381 taagccctca actttatgac atgacgcgct tattagctat aatgcgcaac aattttctta
    13441 gagtaaaagt gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct
    13501 gttaaaaact gaactggaaa acctgggggc cgttgaatgc caggtggttc agggtggggt
    13561 ccatttcaag ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc
    13621 gcgtattatg ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg
    13681 tgttcaggcg atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt
    13741 cagtggtttg aatgacacca tacgcaacag tcagtacggt gcgatgaaag tgaaagacgc
    13801 gatcgtcgat gctttcacgc ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc
    13861 ggatatccgc gttaacgtct ggctgcataa agaaaccgcc agtatcgccc ttgatctcag
    13921 tggtgatggt ttacatctgc gtggctatcg cgatcgtgct ggcattgcgc cgatcaaaga
    13981 aaccctggca gccgctattg tgatgcgatc cggctggcag ccaggaacac cactgctcga
    14041 tccaatgtgt ggttccggta cgttgctgat tgaagcagcg atgctggcga ccgaccgcgc
    14101 accgggcttg caccgtggcc gttggggctt tagcggctgg gcgcagcatg atgaagctat
    14161 ctggcaggaa gtgaaagcgg aagcgcaaac tcgtgcccgt aaaggcctgg cggagtatag
    14221 ttcccatttc tacggttcgg acagtgacgc acgggtgatt caacgtgcgc gcactaacgc
    14281 ccgtcttgcg gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac
    14341 caatccgctg ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg
    14401 tctggacagc gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa
    14461 ccagttcggt ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct
    14521 gcagctgcgt gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa
    14581 aaactaccat gttgccgaaa gcacaccaga cagcaaaccg gcaatggcag cggaggacta
    14641 cgccaaccgt ctgcgtaaga acctcaaaaa attcgagaag tgggctcgcc aggaaggaat
    14701 tgaatgttac cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta
    14761 tgccgactgg gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc
    14821 gcgtcagcgt ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa
    14881 caaactggta ctgaaaaccc gtgaacgcca gaagggcaaa aatcaatacc agaaactggg
    14941 cgagaagggc gagtttctgg aagttaccga atataacgct cacttgtggg tgaacctgac
    15001 ggattacctc gataccggtc tgttcctcga tcaccgtatc gcccgtcgta tgctcggtca
    15061 gatgagcaaa ggcaaagatt tcctcaacct gttctcttat accggcagcg ccaccgtgca
    15121 tgcgggatta ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga
    15181 gtgggcagaa cgcaacctgc gtctgaatgg cctaaccggg cgtgcgcatc gcctgattca
    15241 ggccgattgc ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga
    15301 tccgccaacc ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca
    15361 tctggcgctg atgaaagatt tgaaacgtct gctgcgtgca ggcgggacga tcatgttctc
    15421 gaacaacaaa cgtggcttcc gtatggatct cgacggactg gcaaaactag gactgaaagc
    15481 acaagaaatt acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa
    15541 ctgctggctg attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc
    15601 gcatggctgt cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat
    15661 aacgaacgtg tttgtctggt gggccgcaac ggcgcaggca aatcgacgtt aatgaaaatc
    15721 ctcaaccgtg aacaagggct ggatgacggt cgcattattt acgagcaaga tttgattgta
    15781 gctcgtctgc aacaggatcc gccgcgtaac gttgagggga gtgtttatga tttcgttgcc
    15841 gaaggcattg aagaacaagc ggaatacctg aaacgctatc acgatatttc gcgcctggtg
    15901 atgaacgacc cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat
    15961 caccacaacc tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta
    16021 gatcctaatg ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga
    16081 cgcgcgctgg tgagcaatcc gcgcgtattg ctgctcgacg aaccgactaa ccacctggat
    16141 atcgaaacca tcgactggct ggaagggttc cttaaaacct tcaatgggac gattattttc
    16201 atctcccacg accgctcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc
    16261 ggcaagctgg tgacctatcc ggggaattac gaccagtacc tgctggaaaa agaagaagcc
    16321 ctgcgcgtgg aagaactaca aaatgccgag ttcgatcgca aactggcgca ggaagaggtg
    16381 tggatccgcc aggggatcaa agcacgtcgt acccgtaatg aaggccgcgt acgcgccctg
    16441 aaagcaatgc gtcgcgagcg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag
    16501 gtggaagaag ccagccgctc cggtaagatc gttttcgaaa tggaggacgt ttgctaccag
    16561 gttgacggta agcaactggt gaaagatttt tctgcccagg ttctacgcgg cgacaaaatt
    16621 gccctgatag gtccgaatgg gtgcggcaaa accacgctgc tgaaactaat gctcggtcag
    16681 cttcaagcag acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat
    16741 cagcaccgcg cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag
    16801 caagaggtga tggttaacgg caagccacgc cacgtattgg gctatttgca ggacttcctg
    16861 ttccatccaa aacgggcgat gacgccggta cgtgcgcttt ctggcggtga gcgtaaccgc
    16921 ttgttgttgg cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc
    16981 aacgatcttg atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc
    17041 acggtattgc tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg
    17101 atcttcgaag gcggcggtaa aattggtcgt tatgtcggcg gttatcatga tgcccgtggt
    17161 cagcaagaac agtatgtggc gctcaaacag cctgcggtga aaaaaagcga agaagccgcc
    17221 gcgccaaaag cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc
    17281 gaactggagc agctaccgca attgcttgaa gaactggagg cgaagctgga agctttacag
    17341 acgcaagtgg cggatgcttc cttcttcagt cagccccatg agcagacgca aaaagtgctc
    17401 gctgatatgg ctgctgcaga gcaggaactg gagcaagcct ttgaacgctg ggagtatctt
    17461 gaagcgttaa aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag actgctgaca
    17521 acggtaaaag tgcctgatgt actgggttta tcaggcatga aaaccaacac tttgttagtc
    17581 atctgcaaaa tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag
    17641 cacatcctgt gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag
    17701 aaagcggcat gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag
    17761 cgtcccaccg cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct
    17821 tttgtgaata tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc
    17881 gtgctttttt ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg
    17941 gttcccgcgt tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc
    18001 cgtttaaaag agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag
    18061 attttcctcg cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg
    18121 gtaggcagca gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag
    18181 tgcgttgatc gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg
    18241 ctaaaaccag gtgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca
    18301 atccttcctg ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt
    18361 agaaacagcc tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg
    18421 gctaatattt tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt
    18481 ctcgctgggg tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgattttt
    18541 ctggccagta ttatggtgcc aacgttaaag atgatcgcca ttgcgtggct gtgttgggat
    18601 gccaaaggac acggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag
    18661 tttgtaggcc gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg
    18721 cgtatgggag gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc
    18781 gtcataatga caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa
    18841 cctgaatcag agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat
    18901 ccagaaagtg aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg
    18961 ggcctgggtt cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc
    19021 gaatgcggaa ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg
    19081 cgtggttgaa agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct
    19141 gaattccggt atggaaaaat tgctgcataa agataccgtc ttttgggtgg tgaaaccgca
    19201 gattggtcga gaagggatta gcggcctggg aacgctactg tctggggttt atatcgaact
    19261 gcagccaggc gcgaaaggca gcaaaatgga taaatacgat ttgttggact cgccaccgtt
    19321 ggccccgcct gatgcgaaag gtatccgtgt gcttctcgat agcaaaaaag ccgggcagct
    19381 ctcgccagga gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac
    19441 tttcgatacg caaaaacgca atatcagcta tcaactgttc atcaatgcac cttatgaccg
    19501 actggtgacc agcaatgttc gtttctggaa agatagtggc attgcggttg atctgacgtc
    19561 agcgggaatg cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgtcagctt
    19621 tgatgtgccg gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt
    19681 gtatgatgat cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat
    19741 gttctttaaa gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat
    19801 tcgcctgggt accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa
    19861 cgatgattac cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct
    19921 tggcgaaaat gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg
    19981 atcgctgaaa accggaaacc tggtcactgg cgcactgtat gttgatctcg atttctatcc
    20041 aaatacgcct gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgaccgt
    20101 cagcggcggc ctggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa
    20161 actgccactg aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac
    20221 aatgaaaaac ctgcaaacga cgctggatag catgaacaag atccttgcca gccagtcgat
    20281 gcagcagtta ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg
    20341 cttccagcct ggttccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca
    20401 ggtgttgcga gaactgcaac cggtgctgaa aacgctcaac gagaagagta acgcgctggt
    20461 atttgaagcg aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg
    20521 ctagtgacga ttgcggcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac
    20581 tattaccagt tacctgtggt gcagagcggt acacaaagca ccgccagcca gggtaatcgc
    20641 ctgttatggg ttgagcaggt cgctgttcct gactatctgg cggggaatgg tgtggtttat
    20701 caaaccagtg atgtgaagta tgtgattgcc aacaacaacc tgtgggccag cccgttggat
    20761 caacagttgc gcaacaccct ggttgccaac ctgagtacgc aactacccgg ctgggtggtt
    20821 gcttcccagc ctctgggaag cgcccaggac acgctcaatg ttaccgtcac ggagtttaac
    20881 ggtcgctatg atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa
    20941 ctgatcaaac gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg
    21001 gttaaagtgc tggccagtgt ctggagtcag gaagccgctt ctattgcgca agagataaag
    21061 cgtctacctt aatcataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg
    21121 gtttttttgt ttctaacgtg cggaaaaagt tgttcctctt cacatttttt gtacaaccga
    21181 catgcccgtg tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta
    21241 tgattcgcgg tattgcttaa ctgtgattgc atatttagta atcactgttt tcttttccac
    21301 cagaaaccag tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg
    21361 gcacatcaac gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat
    21421 cagacgctga atcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg
    21481 gtagtaatgg cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc
    21541 tttatatact ctgtctgatt aaaatcagaa tgcagacgta tcctggaaca gacctacttt
    21601 caggtcgttg gcggtataga tcaaacgacc atcaaccagc acttcgccat cagccaggcc
    21661 cataatcaga cgacggttaa ctatgcgttt aaagtgaata cgataggtca cttttttcgc
    21721 cgtcggcagc acctggcctg tgaatttcac ttcacctacg cccagcgcgc gacctttacc
    21781 ttcgccgccc agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag
    21841 gcatcccggc ataaccggat cgccgataaa gtggcatccg aagaaccaca gatccggatt
    21901 gatatccagc tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt
    21961 gaccacacgg tccatcatca gcatgtttgg tgctggcaat tgcgggcctt tagcgccaaa
    22021 cagttcaccg cgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac
    22081 catgttctct gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg
    22141 aacaagtccg atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg
    22201 tgacgtcctt cctgttgcga tgcttgtgcg atacgttcct ggatggtttg catcagcgtc
    22261 gtttggcctt cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg
    22321 tctactgccc agatggtgaa tttgtcttct tctaccgctt tcaccagttc actgtgaaga
    22381 cttaaatggc gaacgttagc tgtggggata atgacacctt gtttccccgt taactcacgt
    22441 tgctggcaaa tagcaaagaa gccttcgatt ttctcattta aaccaccgac tggctgggcg
    22501 cgaccgaact gatcgactga acctgtgata gcgatactct gattcaccgg cacatcggcg
    22561 agggcgctta tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg
    22621 tatgactgct caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc
    22681 gacatcagga acgcttgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc
    22741 gctttgcgtt cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga
    22801 gaaggttcgc caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt
    22861 tgcccgatgc gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc
    22921 atgcgttcag cgaggaaacc ttcgcgccat tcacgctgct gtagcattaa gtttagctgc
    22981 tcgccggaga aggtgtcgcc atcacacagg gaggcgacct cttgacactg gcggaggatc
    23041 cactgcgggc taagcggaag tgtttcttgt tcaccagtgt agcgtgctgc ttcgcggata
    23101 agtaccggcc aggcatccgc tcccggtgct ggtaagtgat tatgtctggc ggtaaatgtc
    23161 acccagcgac accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat
    23221 tcgctataaa tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat
    23281 tcgcgttcgc ctaccagaat gactttcagc ttcaatggca tcgaaggcac agagacgggg
    23341 agagggcgcg actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc
    23401 agccgcatcc acagcagagg ttgcgccagc agtgtacgta aagagatgat gagaataccg
    23461 ccatttgcct gatgcaccag accaggctgc agggtaatat cgccattaaa ctgacgcagg
    23521 cagccaaaga gttgctctgc ttctacccag tcggcagcga caacttgcgt taaagtcgca
    23581 aaattatcat ctgcactcac tgcgtgacgt aagcggatgg tgtggccgga aacctcatag
    23641 tgaccgccca ccagttggcc tgcatcgctt tgtagcatac gcgcggcatc agcaataaga
    23701 ttcagatact cagactcttc cggggccttc gccagcataa aagaggagga tgctcgcgta
    23761 tgcagcaact gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggagcg
    23821 ttttcgtcaa tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc
    23881 aggtcacgcc atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa
    23941 gccgcgatta taccgtaacc ggcactgcag cacacgtaga aagcaacgac aatactcctg
    24001 gcatgggcgt taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat
    24061 attctgtaaa gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa
    24121 tcttgaaagc ggttggaaat ggaagtatct ggtgaagaag caccgcgaag gggagttaat
    24181 cacccgttac atagaagcca gtgccgccca ggaagccgtc gatgagttgc tctcgctgga
    24241 aaatgaaccc gtgctggtaa atggttggat tgacaagcat atgaatccgg aactggtcaa
    24301 tcgcatgaag cagaccattc gggccagacg gaagcgccat tttaatgcag aacaccagca
    24361 tacgcgcaaa aaatcgatcg atctggaatt tatcgtctgg caacgtctgg ctggccttgc
    24421 gcagcgtcgc ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa
    24481 caaagagaaa tacgcgaata aaatgtcttc tttgaagcag gatctgcagg cattgctggg
    24541 taaggaataa ctgtcaaaag tcagttcaat ttactaaagg cgaaaaaaaa accccgcagc
    24601 tgcggggttt ttctaccaga cgagaactta agcctgcggc tgagttacaa cgtctttgat
    24661 gcctttaact tcgatctcta cgcgacgatc cggagccagg caatcgatca gtgcagcacg
    24721 ctgtttcacg ttgtcacagg tgttgccagt aaccgggttg gattcgccca taccacgtgc
    24781 ggagattttg tctgccggga tacctttaga gatcaggtaa tcaacaacgg actgagcacg
    24841 acgctcggac agagcctggt tataagcgtc agaaccgatg cggtcagtgt aacccagaac
    24901 aactacggaa ccgtctttcg gatccaggtt gctcagctgg ctgtacagct gatccagagc
    24961 agcctgacct tccggtttca gggttgcttt gttgaaggtg aacagaacgt cagacttcag
    25021 agtgaagtgc ttggtctgta cttccggtgc cggagctgga gccggagcaa ctactggagc
    25081 tacttcgccc tgaccgaaac ggtaggaaac acccaggctc agcatgccgt tgtccggacg
    25141 agtgccgatg gtgtgtgcgt caccgatgtt gttggtccac tggtattcca gacgggtagc
    25201 gatttcagga gtgatcgcat actcaacacc gccagcgaag accggagaaa cgccggtgtc
    25261 gtggttttta ccataaacgt tggatttagt gtctgcacgc cataccatac cacccagacg
    25321 agtgtatacg tccagatcgt cagtgattgg gtaacccagt ttagcggtca gctgaacgcc
    25381 ctgagcttta tatgcaccgt tttcaacgct gcctttgtat ggcatacgac ctaaccagtc
    25441 ataacccatt tcaaagccaa catacgggtt aacctggtaa ccaccaaaag caccagcgcc
    25501 cagttggttt tcatgggtcg gaccattgtt gtcgatgaaa ccagtgtcat ggtactggga
    25561 ccagcccagt ttagcaccag tgtaccaggt gttatctttc ggagcggcct gcgctacggt
    25621 agcgaaacca gccagtgcca ctgcaatcgc gatagctgtc tttttcattt tttgcgcctc
    25681 gttatcatcc aaaatacgcc atgaatatct ccaacgagat aacacggtta aatccttcac
    25741 cggggatctg ctcaatatta actctaccga tatcttcggc ttatgccgag cacccctggc
    25801 gatgtaaagt ctacaacgta gttgaaaact tacaagtgtg aactccgtca ggcatatgaa
    25861 aaaaaagtct tgtataaggt acgtttaatc ttttttgtca gcgacaattt acagaagaaa
    25921 atcgtggaaa ccgcttcaga caagcctccg caaggaaaat tagtcacgac tgaaagcatt
    25981 ggctgggcga taaaaaaagt tccaggatta atcctaaatt tacttaatga tacaaattag
    26041 agtgaatttt tagcccggaa agttgtctcg ttgcgtgaga ggatgcgctt accggacgca
    26101 taataaaccc catagcgtta ccttcatttg ccgcatcaac aagttcagca tgctcttctg
    26161 cagtcaaatc atctgccaac caaccgatca ccacgctgta attgcccgtg cgtaaagcgc
    26221 gaaccattga ctccacggtg tggcaagggg agagctggct aatctgcatt actttcgtta
    26281 agggtagccc agatgcctga acccattcgc gactcagttt ttgttgcggt gttaaccaga
    26341 gttgccagcg cgattgctga ccgagttgct gtaacaatgg caacagtaga agttgcgtca
    26401 tcatgggctg atcttcgcga tagacaactt cactgataag cccggctgta gtgttttccg
    26461 tagagacacg cgcaatttta ctttctgcgg atgagaacga cgaagaacga tgtgcatagc
    26521 ctgaagtgta cataatcaat ccagccctgt gagttactgt atgaatatac agtacatcca
    26581 gtgacgacaa agatcaactc tattttcgga aagagcctcg caaattttgt cgttggtgac
    26641 gggaaaacat aaattaatct tgccccttaa gaataagttg cctattttcg tagttaacgg
    26701 atccgttaat gtgaatcatt cttttatgtt atgat
//
LOCUS       DADSZD010000052        26729 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.052, whole genome shotgun sequence.
ACCESSION   DADSZD010000052 DADSZD010000000
VERSION     DADSZD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.052"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            36..800
                     /locus_tag="J1Z28_001926"
     CDS             36..800
                     /locus_tag="J1Z28_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA7716763.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            973..1656
                     /gene="cmk"
                     /locus_tag="J1Z28_001927"
     CDS             973..1656
                     /gene="cmk"
                     /locus_tag="J1Z28_001927"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBA7716764.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1767..3440
                     /gene="rpsA"
                     /locus_tag="J1Z28_001928"
     CDS             1767..3440
                     /gene="rpsA"
                     /locus_tag="J1Z28_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBA7716765.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3600..3884
                     /gene="ihfB"
                     /locus_tag="J1Z28_001929"
     CDS             3600..3884
                     /gene="ihfB"
                     /locus_tag="J1Z28_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBA7716766.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4090..6354
                     /locus_tag="J1Z28_001930"
     CDS             4090..6354
                     /locus_tag="J1Z28_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBA7716767.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTVTLAFLTLFACVLAFIPV
                     KTVRYIVLTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASIQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLIPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWWISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6391..8139
                     /gene="msbA"
                     /locus_tag="J1Z28_001931"
     CDS             6391..8139
                     /gene="msbA"
                     /locus_tag="J1Z28_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBA7716768.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8136..9122
                     /gene="lpxK"
                     /locus_tag="J1Z28_001932"
     CDS             8136..9122
                     /gene="lpxK"
                     /locus_tag="J1Z28_001932"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBA7716769.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PIPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAIAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGH"
     gene            9159..10391
                     /locus_tag="J1Z28_001933"
     CDS             9159..10391
                     /locus_tag="J1Z28_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBA7716770.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGDYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPEWNDERDLVSQAEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYIPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     SQGLFTDCRAGWEIDPVA"
     gene            10443..10625
                     /locus_tag="J1Z28_001934"
     CDS             10443..10625
                     /locus_tag="J1Z28_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBA7716771.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10622..11368
                     /gene="kdsB"
                     /locus_tag="J1Z28_001935"
     CDS             10622..11368
                     /gene="kdsB"
                     /locus_tag="J1Z28_001935"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBA7716772.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLESVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLKRVRAEMR"
     gene            11522..12415
                     /locus_tag="J1Z28_001936"
     CDS             11522..12415
                     /locus_tag="J1Z28_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBA7716773.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSAPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12392..13171)
                     /gene="elyC"
                     /locus_tag="J1Z28_001937"
     CDS             complement(12392..13171)
                     /gene="elyC"
                     /locus_tag="J1Z28_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBA7716774.1"
                     /translation="MLFTLKKVIGNMLLPLPLILLIIGAGLALLWFSRFQKTGRIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            13307..14092
                     /gene="cmoM"
                     /locus_tag="J1Z28_001938"
     CDS             13307..14092
                     /gene="cmoM"
                     /locus_tag="J1Z28_001938"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBA7716775.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKL"
     gene            14089..15411
                     /gene="mukF"
                     /locus_tag="J1Z28_001939"
     CDS             14089..15411
                     /gene="mukF"
                     /locus_tag="J1Z28_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBA7716776.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15392..16096
                     /gene="mukE"
                     /locus_tag="J1Z28_001940"
     CDS             15392..16096
                     /gene="mukE"
                     /locus_tag="J1Z28_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBA7716777.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            16096..20556
                     /gene="mukB"
                     /locus_tag="J1Z28_001941"
     CDS             16096..20556
                     /gene="mukB"
                     /locus_tag="J1Z28_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBA7716778.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRKQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20817..22664
                     /gene="ldtD"
                     /locus_tag="J1Z28_001942"
     CDS             20817..22664
                     /gene="ldtD"
                     /locus_tag="J1Z28_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA7716779.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMTRDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKLMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLAREDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22845..23393
                     /locus_tag="J1Z28_001943"
     CDS             22845..23393
                     /locus_tag="J1Z28_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBA7716780.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23420..24067
                     /gene="gloC"
                     /locus_tag="J1Z28_001944"
     CDS             23420..24067
                     /gene="gloC"
                     /locus_tag="J1Z28_001944"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBA7716781.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24117..25307)
                     /locus_tag="J1Z28_001945"
     CDS             complement(24117..25307)
                     /locus_tag="J1Z28_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HBA7716782.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25492..26580)
                     /gene="ompF"
                     /locus_tag="J1Z28_001946"
     CDS             complement(25492..26580)
                     /gene="ompF"
                     /locus_tag="J1Z28_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBA7716783.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6485 a         6539 c         7251 g         6454 t
ORIGIN      
        1 aatgtttata tataattaat caacggatga ttcacatgaa gaatactaaa ttattgctgg
       61 cgattgcgac ctctgcagcc ttactgacag gatgtcagaa tacgcacggt attaataccg
      121 atttggctat cagctcaggt ctgaatgctt ataaagccgc aacattaagc gatgctgatg
      181 ctaaagcaat cgccaatcag ggctgtgtcg aaatggacag cggcaataaa gtggcaagca
      241 aatccagcaa gtacgggaaa cgtctggcaa aaatcgccaa agcattgggt aacaatatta
      301 atggcacgcc agtcaactat aaggtttata tgaccagcga cgtcaacgca tgggcgatgg
      361 cgaacggttg tgttcgtgtc tacagtggcc tgatggacat gatgaacgac aacgaaattg
      421 aaggcgttct gggccatgaa ctgggccacg tcgcgttggg tcactcgctg gctgaaatga
      481 aagcttctta tgcgatcgtg gccgcacgcg atgccatttc agctaccagc ggtgtggctt
      541 cccagctttc ccgttcacaa ttaggtgata tcgcagaagg cgctatcaat gctaaatact
      601 cccgtgataa agaatccgaa gcagatgatt tctcttttga tctgttgaag aaacgtggca
      661 tcagtaccca ggggctggtt ggtagctttg aaaaactggc cagcctggat ggcggtcgca
      721 cccagtccat gtttgattct cacccaccat caacagagcg tgcgcaacac atccgtgatc
      781 gtatcgcctc tggtaagtaa atcattgtca tctttcgggc tggtcttctg ccagcccgct
      841 ataattgcgc aataaatccc catctgaata cagataaaac tggtttttgc acacaacgtt
      901 aacgatttgt ggcgtcggcg cgtataatgc gcgcggttat gttaacggta cgcctgtttt
      961 aaggagataa agatgacggc aattgccccg gttattacca ttgatggccc aagcggtgca
     1021 gggaaaggca ccttgtgtaa ggctatggcg gaagcgttgc aatggcatct gctggattcg
     1081 ggtgcaattt atcgcgtact ggcactagcg gcattacatc accatgttga tgttgcatcg
     1141 gaagatgcgc tggtgccgct ggcatcccat ctggatgtac gttttgtgtc aaccaacggt
     1201 aatctggaag tgatcctcga aggggaagat gtcagcggcg aaattcgtac tcaggaagtg
     1261 gcgaatgctg cttcacaagt cgcggcattc ccacgcgttc gtgaagcatt attgcgtcgc
     1321 caacgcgcgt ttcgcgaatt accaggcctg attgccgatg gccgcgacat gggaacggtg
     1381 gtattccctg atgcgccagt gaaaattttc cttgacgcct cctcggaaga acgtgcgcat
     1441 cgccgcatgc tacagttgca ggagaagggc tttagtgtta actttgagcg ccttttggcc
     1501 gagatcaaag aacgcgacga ccgcgatcgt aaccgagcgg tagcgccact ggttccggca
     1561 gccgatgctt tagtgttgga ttccaccacc ttaagcattg agcaagtgat tgaaaaagcg
     1621 ctacaatacg cgcgccagaa attggctctc gcataagcga ccgaatttgc agtacccccg
     1681 ttgcaatgga atgacagcgg gtatgttaaa caaccccatc cggcatggag ccaggtggac
     1741 gttaaatata aacctgaaga ttaaacatga ctgaatcttt tgctcaactc tttgaagagt
     1801 ccttaaaaga aatcgaaacc cgcccgggtt ctatcgttcg tggcgttgtt gttgctatcg
     1861 acaaagacgt agtactggtt gacgctggtc tgaaatctga gtccgccatc ccggctgagc
     1921 agttcaaaaa cgcccagggc gagctggaaa tccaggtagg tgacgaagtt gacgttgctc
     1981 tggacgcagt agaagacggc ttcggtgaaa ctctgctgtc ccgtgagaaa gctaaacgtc
     2041 acgaagcctg gatcacgctg gaaaaagctt acgaagatgc tgaaactgtt accggtgtta
     2101 tcaacggcaa agttaagggc ggcttcactg ttgagctgaa cggtattcgt gcgttcctgc
     2161 caggttctct ggtagacgtt cgtccggtgc gtgacactct gcacctggaa ggcaaagagc
     2221 ttgaattcaa agtaatcaag ctggatcaga agcgcaacaa cgttgttgtt tctcgtcgtg
     2281 ccgttatcga atccgaaaac agcgcagagc gcgatcagct gctggaaaac ctgcaggaag
     2341 gcatggaagt taaaggtatc gttaagaacc tcactgacta cggtgcattc gttgatctgg
     2401 gcggcgttga cggcctgctg cacatcactg acatggcctg gaaacgcgtt aagcatccga
     2461 gcgaaatcgt caacgtgggc gacgaaatca ctgttaaagt gctgaagttc gaccgcgaac
     2521 gtacccgtgt atccctgggc ctgaaacagc tgggcgaaga tccgtgggta gctatcgcta
     2581 aacgttatcc ggaaggtacc aaactgaccg gtcgcgtgac caacctgacc gactacggct
     2641 gcttcgttga aatcgaagaa ggcgttgaag gcctggtaca cgtttccgaa atggattgga
     2701 ccaacaaaaa catccacccg tccaaagttg ttaacgttgg cgatgtagtg gaagttatgg
     2761 ttctggatat cgacgaagaa cgtcgtcgta tctccctggg tctgaaacag tgcaaagcta
     2821 acccgtggca gcagttcgcg gaaacccaca acaagggcga ccgtgttgaa ggtaaaatca
     2881 agtctatcac tgacttcggt atcttcatcg gcctggacgg cggcatcgac ggcctggttc
     2941 acctgtctga catctcctgg aacgttgcag gcgaagaagc agttcgtgaa tacaaaaaag
     3001 gcgacgaaat cgctgcagtt gttctgcagg ttgacgcaga acgtgaacgt atctccctgg
     3061 gcgttaaaca gctcgcagaa gatccgttca acaactgggt tgctctgaac aagaaaggcg
     3121 ctatcgtaac cggtaaagta actgcagttg acgctaaagg cgcaaccgta gaactggctg
     3181 acggcgttga aggttacctg cgtgcttctg aagcatcccg tgaccgcgtt gaagacgcta
     3241 ccctggttct gagcgttggc gacgaagttg aagctaaatt caccggcgtt gatcgtaaaa
     3301 accgcgcaat cagcctgtct gttcgtgcga aagacgaagc tgatgagaaa gatgcaatcg
     3361 caactgttaa caaacaggaa gatgcaaact tctccaacaa cgcaatggct gaagctttca
     3421 aagcagctaa aggcgagtaa ttctctgact cttcgggatt tttattccga agtttgttga
     3481 gtttacttga cagattgcag gtttcgtcct gtaatcaagc actaagggcg gctacggccg
     3541 cccttaatca atgcagcaac agcagccgct taatttgcct ttaaggaacc ggaggaatca
     3601 tgaccaagtc agaattgata gaaagacttg ccacccagca atcgcacatt cccgccaaga
     3661 cggttgaaga tgcagtaaaa gagatgctgg agcatatggc ctcgactctt gcgcagggcg
     3721 agcgtattga aatccgcggt ttcggcagtt tctctttgca ctaccgcgca ccacgtaccg
     3781 gacgtaatcc gaagactggc gataaagtag aactggaagg aaaatacgtt cctcacttta
     3841 aaccaggtaa agaactgcgc gatcgcgcca atatttacgg ttaagttttt actcaaactt
     3901 gaacgcgaga aaagcacctg tcgggtgctt ttttcatttc tctaatctgg aactggaagc
     3961 tgcctcgcag agttttgagc agttttcacc ctatcgttaa attcttctga acgtgcctcg
     4021 gggaacgcaa aactcccacc agacaaccgt tcaacaaagt tgcacacttt ctataaacag
     4081 ggagggtgca tgaaaataac gacagtcggc gtatgcataa tttgcggaat ttttccgttg
     4141 ctgattttgc cccaattgcc tgggacagta acccttgcgt ttctgactct cttcgcctgt
     4201 gtactggcat ttatccctgt taaaaccgtc cgttatatcg tgctgacgtt gctgtttttc
     4261 gtttggggca tattagcagc aaagcaaatt ttgtgggcag gagaaacctt aactggcgcg
     4321 acgcaggatg caattgttga gatcattgct actgacggca tgaccactca ttacggtcaa
     4381 attactcatc tacaaggtcg acgtatattc cctgcgccag gcctcgtact gtatggcgaa
     4441 tatcttccgc aagcggtttg tgccggacaa gtatggtcaa tgaaactcaa agttcgtgca
     4501 gttcatggtc aacttaatga tggcggcttt gatagccagc gttatgctat tgcccagcat
     4561 caaccgctca ccggccgttt tctgcaggca agtgtcattg aaccgaattg tagcctgcgt
     4621 gcacagtatc tggcgtcaat acaaacaacg ctgcaaccct atatgtggaa cgcggttatt
     4681 cttggattag gtatggggga acggttatcc gtccccaaag aaatcaaaaa tatcatgcgt
     4741 gatactggaa cggcgcattt aatggcaata tcaggattgc acatcgcttt tgcggcgttg
     4801 ctggctgcag gactcattcg cggtgggcaa gtttttctgc ctgggtgctg gatccactgg
     4861 caaatgccat taattggtgg aatctgctgt gctgcttttt atgcctggct tacgggaatg
     4921 caacctcctg cattgcgtac cgtggtggcg cttgccattt ggggaatgct taagttaagt
     4981 ggacgacaat ggagtggctg ggatgtatgg atatgttgtc tggcggcaat tttgctgatg
     5041 gatcctgttg ccattctctc gcaaagttta tggctatctg ccgctgcggt cgcggcactg
     5101 attttttggt atcagtggtt tccctgtccg gagtggcagc tgccgccggt attgcgcgcg
     5161 gttgtttccc tcatccatct gcaactggga atcaccctcc tgcttatacc cgtgcaaatt
     5221 gtcatatttc atggcattag tctgacctcg tttattgcaa atctttttgc aattcccctg
     5281 gtgacattta tcacggttcc gttgattctc gccgcgatgg ttgtgcattt aagcgggccg
     5341 tttattctgg aacaagggtt atggtttctt gccgaccggt ctttggcttt acttttctgg
     5401 gggttaaaga gtttgccgga agggtggatc aacattgctg aacgttggca atggctaaca
     5461 ttttccccat ggttcttact ggtggtatgg cgattaaacg cctggcgaac gttgccagca
     5521 atgtgtgtgg ctgtaggctt gctgatgtgc ttgccgctgt ggcaaaaacc tcgacctgac
     5581 gagtggcaag tgtacatgct tgatgtcggg caagggctgg caatggtcat tgcgcgaaac
     5641 ggcaaagcga ttctctatga tacgggactg gcctggcccg aaggggatag tgggcaacaa
     5701 ctgattatcc cctggctcca ctggcataat cttgaaccgg aaggcgttat tttgagtcat
     5761 gaacatctgg atcaccgggg agggctggac tcaatattgc acacatggcc gatgttatgg
     5821 atccgaagtc cgttaaactg ggaacatcat cagccctgtg tgcgtggcga agcgtggcaa
     5881 tggcaaggat tgcgtttcag cgcgcactgg cctttacaag gtagcaacga taaaggaaat
     5941 aaccattcct gtgtggttaa gattgatgac gggacgaata gcattcttct aaccggtgat
     6001 attgaagccc ccgctgaaca aaagatgcta agccgttact ggcagcaaat gcaggcaaca
     6061 ttacttcagg tacctcacca tggcagtaat acctcatcat cgttgccatt aattcagcga
     6121 gtgaatggaa aagtggcact cgcatcggca tcgcgctata acgcatggcg actgccctca
     6181 agcaaagtta aacatcgcta tcaacaacag ggttataagt ggcttgatac tccacatcag
     6241 ggtcaaataa cggtcaattt ttcagcgcaa ggctggtgga ttagcagcct cagagagcaa
     6301 attttacctc gttggtatca tcagtggttt ggcgtgccag tggataacgg gtagaatatg
     6361 cggctatttc aacaaatgct ggttttttga atgcataacg acaaagatct ctctacgtgg
     6421 cagacattcc gccgactgtg gccaaccatt gcgcctttta aagcgggtct gatcgtggcg
     6481 ggcgtagcgt taatcctcaa cgcagccagc gataccttca tgttatcgct ccttaagcca
     6541 cttcttgatg atggctttgg taaaacagat cgctccgtgc tgatgtggat gccgctggtg
     6601 gtgatcgggc tgatgatttt acgtggtatc accagctata tctccagcta ctgtatctcc
     6661 tgggtatcag gaaaggtggt aatgactatg cgtcgccgcc tgtttggtca catgatggga
     6721 atgccggttt cattctttga caaacagtca acgggtacgc tgttgtcacg tatcacctac
     6781 gattccgaac aggtcgcttc ttcttcttcc ggcgcactga ttactgttgt gcgtgaaggt
     6841 gcgtcgatca tcggcctgtt catcatgatg ttctattaca gttggcaact gtcgatcatt
     6901 ttgattgtgc tggcaccgat tgtttcgatt gcgattcgcg ttgtatcgaa gcgttttcgc
     6961 aacatcagta aaaacatgca gaacaccatg gggcaggtga ccaccagcgc cgaacaaatg
     7021 ctgaaaggcc ataaagaagt attgattttc ggtggtcagg aagtggaaac gaaacgcttc
     7081 gataaagtca gcaaccgaat gcgtcttcag gggatgaaaa tggtttcagc ctcttccatc
     7141 tctgatccga tcattcagct gatcgcctct ttggcgctgg cgtttgttct gtatgcggca
     7201 agcttcccaa gtgtcatgga tagcctgact gccggtacga ttaccgttgt tttctcttca
     7261 atgattgcac tgatgcgtcc actgaaatcg ctgaccaacg ttaacgccca gttccagcgc
     7321 ggtatggcgg cttgtcagac gctgtttacc attctggaca gtgagcagga gaaagacgaa
     7381 ggtaagcgcg tgatcgagcg tgcgactggc gatgtggaat tccgcaacgt cacctttact
     7441 tatccgggac gtgacgtacc cgcattgcgt aacatcaacc tgaaaattcc ggcagggaag
     7501 acggttgctc tggttggacg ctctggttca ggtaaatcaa ccatcgccag cctgatcacg
     7561 cgtttttacg atattgatga aggcgaaatc ctgatggatg gtcacgatct gcgcgagtat
     7621 accctggcgt cgttacgtaa ccaggttgct ctggtgtcgc agaatgtcca tctgtttaac
     7681 gatacggttg ctaacaacat tgcttacgca cggactgaac agtacagccg tgagcaaatt
     7741 gaagaagcgg cgcgtatggc ctacgccatg gacttcatca ataagatgga taacggtctc
     7801 gatacagtga ttggtgaaaa cggcgtgctg ctctctggcg gtcagcgtca gcgtattgct
     7861 atcgctcgag ccttgttgcg tgatagcccg attctgattc tggacgaagc tacctcggcg
     7921 ttggataccg aatccgaacg tgcgattcag gcggcactgg atgagttgca gaaaaaccgt
     7981 acctctctgg tgattgccca ccgcttgtct accattgaaa aggcagacga aatcgtggtc
     8041 gtcgaggatg gtgtcattgt ggaacgcggt acgcataacg atttgcttga gcaccgtggc
     8101 gtttacgcgc aacttcacaa aatgcagttt ggtcaatgat cgaaaaaatc tggtctggtg
     8161 aatccccttt gtggcggcta ttgctgccac tctcctggtt gtatggcctg gtgagtggcg
     8221 cgatccgtct ttgctataaa ctaaaactga agcgcgcctg gcgtgccccc ataccggttg
     8281 tcgtggttgg taatctcacc gcaggcggca acggaaaaac cccggtcgtt gtctggctgg
     8341 tggaacagct gcaacagcgc ggtattcgcg tgggggttgt atcgcgggga tatggaggta
     8401 aggctgaatc ttatccgctg ttattgtcgg cagataccac cacagcacag gcgggtgatg
     8461 aacctgtgtt gatttatcaa cgcactggcg cacctgttgc ggtttctccc gtgcgttctg
     8521 acgcggtaaa agccattctg gcgcaacacc ctgatgtgca gatcatcgta accgacgatg
     8581 gtttacagca ttatcgtctg gcgcgtgatg tggaaattgt cgttattgat ggtgtgcgtc
     8641 gctttggcaa tggctggtgg ttgccggcgg ggccaatgcg tgagcgagcg gggcgcttaa
     8701 agtcggttga tgcggtaatc gtcaacggcg gtgtcccccg cagcggtgaa atccccatgc
     8761 atctgctgcc gggtcaggcg gtgaatttac gtaccggtac gcgttgtgac gttgctcagc
     8821 ttgaacatgt ggtggcgatt gcggggattg ggcatccgcc gcgctttttt gccacgctga
     8881 agatgtgcgg cgtacaaccg gaaaaatgtg taccgctggc cgatcatcag tctttgaatc
     8941 atgcggatgt cagcgcgttg gtaagcgccg gacaaacgct ggtaatgact gaaaaagatg
     9001 cggtgaaatg ccgggccttt gcagaagaaa attggtggta tttgcctgta gacgcacagc
     9061 tttcaggtga tgaaccagcg aaactgcttg cgcaactaac ctcgctggct tctggccact
     9121 agttacgccg cggcagcgtt cgattgatgg agtcatgaat gtcgctgccg cacctctccc
     9181 ttgctgatgc gcgtaatctt caccttgccg cgcaaggcct gttaaacaaa ccccgccgtc
     9241 gagcgtcgtt ggaggatatt ccggcaacga tctcccgcat gtccttgctg caaatcgata
     9301 ccatcaatat tgttgcccgt agcccatatc tggtgctttt cagccgtctg ggagattatc
     9361 ctgcgcagtg gctggatgag tctctggcgc gtggcgaatt aatggaatac tgggcgcatg
     9421 aagcctgctt tatgccgcgt agcgattttc gtcttattcg ccaccgcatg ctggcacctg
     9481 aaaaaatggg ctggaaatat aaagacgcct ggatgcagga acatgcggcg gaaattgcac
     9541 agttaattca gcatattcat gataaggggc cagtacgttc agccgatttt gagcatcctc
     9601 gtaaaggggc gagcggctgg tgggagtgga agccgcataa acgacatctg gaaggtttat
     9661 ttactgccgg aaaggtgatg gtgattgaac ggcgcaactt ccagcgcgtt tatgatttaa
     9721 ctcaccgtgt catgcctgag tggaatgatg agcgcgatct cgtttcgcaa gcagaagcag
     9781 aaatcatcat gctggataac agtgcgcgta gcctgggcat attccgcgaa cagtggctgg
     9841 cagattacta ccggctaaaa cgtccggcac tggcggcgtg gcgtgaagcg agggctgaac
     9901 agcagcaaat cattgctgtg catgttgaaa aattgggtaa tctttggctg catgctgatt
     9961 tgctgccgct actcgagcgt gcgctcgccg gaaagctcac tgcaacgcac agcgcggtac
    10021 tttcgccttt tgatcctgtt gtctgggatc gcaaacgcgc ggagcagctt tttgatttta
    10081 gctaccggct ggagtgctat atcccagcgc cgaaacgcca gtatggctat tttgttctgc
    10141 cgttattaca tcgtgggcaa ttagttgggc gaatggatgc caaaatgcat cgccagacag
    10201 gcatccttga agttatctct ctgtggttac aggaaggcat taaaccgacg acaacgctgc
    10261 aaaaagggtt acgtcaggcg attactgatt tcgctaactg gcagcaggca acgcgggtga
    10321 cattaggacg ctgctcgcaa ggcctcttta ctgattgccg cgccggctgg gaaatagacc
    10381 ccgtcgcata aatgattatg ataagattta tgcattcata acccgatcca tctggaggaa
    10441 ctatggatca tcgtctgctt gaaatcattg cctgcccggt ttgcaacgga aaactttggt
    10501 ataaccagga aaaacaagag cttatttgca aactggacaa cctggctttc ccgctgcgcg
    10561 atggcattcc agtgttgctg gaaacggaag cccgcgtgtt gactgctgat gagagtaaat
    10621 catgagtttt gtggtcatta ttcccgcgcg ttacgcgtcc acgcgtctgc ccggtaaacc
    10681 attggttgat attaacggca aacccatgat tgttcatgtt cttgaacgcg cgcgcgaatc
    10741 aggtgccgag cgcatcatcg tggcaaccga tcatgaggat gttgcccgcg ctgttgaagc
    10801 cgctggcggc gaagtatgta tgacgcgcgc cgatcatcag tcaggtacag aacgattggc
    10861 ggaagttgtc gaaaaatgcg cattcagcga cgacacggtg atcgttaatg tgcagggtga
    10921 tgaaccgatg atccctgcga caatcattcg tcaggttgct gataacctcg ctcagcgtca
    10981 ggtgggtatg gcgactctgg cggtgccaat ccacaatgcg gaagaagcgt ttaacccgaa
    11041 tgcggtgaaa gtggttctcg acgccgaagg gtacgcactg tacttctctc gcgccaccat
    11101 tccgtgggat cgtgatcgtt ttgcaaaaga ccttgaaagc gtaggcgata acttcctgcg
    11161 tcatcttggt atttatggct accgagcagg ctttatccgt cgttatgtta cctggcagcc
    11221 aagtccgtta gaacatatcg aaatgttaga gcagcttcgt gttctgtggt acggcgaaaa
    11281 aatccacgtt gctgtcgccc aggaagttcc tggcacaggt gtggataccc ctgaagatct
    11341 caagcgcgtt cgcgccgaaa tgcgctaatt tcacttcacg acactccagc caattttggg
    11401 aggagtgtcg taccgttacg attttcctca atttttcttt tcaacaattg atctcattca
    11461 ggtgacatct tttatattgg cgctcattat gaaagcagta gcttttatga gggtaatctg
    11521 aatggaacag ctgcgtgccg aattaagcca tttactgggc gaaaaactca gtcgtattga
    11581 gtgcgtcaat gaaaaagcgg atacggcgtt gtgggctttg tatgacagcc agggaaaccc
    11641 aatgccgtta atggcaagaa gctttagtgc gcccggaaaa gcccgacaac tggcatggaa
    11701 aaccaccatg ctggcaagaa gtgggactgt ccgtatgccg actatttatg gtgtgatgac
    11761 gcatgaagaa caccccggcc ccgatgtcct gctactggag cggatgcgtg gtgtttcggt
    11821 ggaggcacca gcccgaacac cagaacgctg ggaacagctc aaagaccaaa tcgttgaagc
    11881 cttactggcc tggcaccgtc aggacagtcg cggttgcgtc ggcgcggtcg acaatactca
    11941 ggaaaatttc tggccctcat ggtaccggca acatgttgaa gtgctatgga ccacgctcaa
    12001 tcagttcaat aacaccggtc taacgatgca ggataagcgg atcctgtttc gcactcgcga
    12061 atgtctcccg gcattatttg aaggctttaa tgacaattgt gtactgattc acggtaactt
    12121 ttgtttacgc agcatgttga aagattctcg cagcgatcag ttactggcga tggtcggacc
    12181 gggactaatg ctttgggcac cgcgagaata cgaactgttc cgactaatgg ataattctct
    12241 ggcggaagat ttgctctgga gttatctgca acgcgcgcca gtggcggagt cgttcatctg
    12301 gcggcgttgg ttgtatgtgt tatgggatga agttgcgcaa ctggtcaaca ccggacgatt
    12361 tagtcggcgt aacttcgatc tggcatcaaa atcactcttg ccgtggctcg cctgacgaac
    12421 ctttcagcca ctgccagatc cggccaagcg tttcatagcc aacgcggtcg ctatgcatca
    12481 accaaaccgg agaagggatt gcccgttccc acggattaag cggcgagtcg atggccaact
    12541 gattcgccgg ggctggtagc ggatttaacc cttcctgctg aaaaaagatc atcgcccgcg
    12601 gcagatgtga tgcggaggtc accagcagga aaggtgcgtc accaatcgcc tgtttcactg
    12661 ctgcagcttc ttcttcggta tcttttggca aatccagggt gataatttgc tcgcgcggca
    12721 cgcccagcga ttgcgcaact ctggcaccga cttcggctgt gctcaccgta ttggttttag
    12781 ctacgcctcc cgtgaagatc agttttgatc ccggattttc gcgccataag cgaatacctt
    12841 cattcaggcg gggcagactg ttattgatta aattcgagct tggtgcccac tgcgggttcc
    12901 aggtataacc acctccgagc accacgatat agtccacttt ttgcgaatta ttccatgtcg
    12961 gataggtgct ttcgatggga cgcagcaggc ggtcggctac cggttgcaaa ctcaataaca
    13021 agagcgccag ccacccaatg ctaataaaaa tcctcccggt tttttgaaaa cggctaaacc
    13081 acagcagcgc caggccagcg ccaataatca acaatatcag cggaagtggc agcaacatat
    13141 tgccaatcac ttttttcagt gtaaaaagca tcctttctgg ttcctttttt aaccatatag
    13201 caagggatcg ccagcgatat tacaccagac gggttcattc tcgccgtggc tgtgacaaaa
    13261 tagcgtcttt atcagaaaac gtcttagcca accggtggag aaagcaatgc aggatcgcaa
    13321 ttttgatgat attgcggaaa agttttcccg taacatttac ggcaccacca aagggcagct
    13381 tcgacaggct attctgtggc aggatctcga tcgcgtgctg gcggaaatgg gcccgcaaaa
    13441 actgcgtgtg ctggatgctg gcggtggaga agggcagacc gcaatcaaaa tggccgagcg
    13501 tgggcatcag gtcattttat gcgatctttc tgcgcagatg atcgaccgcg caaaacaggc
    13561 ggcagaagca aaaggtgtga gcgacaacat gcaatttata cattgcgccg ctcaggatgt
    13621 tgcttcgcat ttggaaacgc ccgttgatct gatattgttc catgcggtgc tcgagtgggt
    13681 ggccgatccc cgcagcgtat tacagaccct gtggtcagtg ttgcgtccag gcggcgtgtt
    13741 gtcgttaatg ttctacaatg cgcatggttt gttgatgcat aacatggtcg ccgggaattt
    13801 tgattacgtg caggcgggaa tgccgaaaaa gaaaaaacgg acgctttcgc cagattatcc
    13861 acgcgacccg gcgcaggttt atctgtggct ggaagaagct ggttggcaaa ttctgggtaa
    13921 gacaggcgtt cgcgtgtttc atgattatct gcgcgagaag caccagcagc gcgactgcta
    13981 tgaagcatta cttgaattag aaacgcgtta ttgccgtcag gaaccgtata ttaccctggg
    14041 gcgttatatt catgtcaccg cgcgcaaacc gcagagcaag gataaattat gagtgaattt
    14101 tcccagacag tccccgaact ggttgcctgg gccagaaaaa atgacttctc catctcgctg
    14161 ccggtagacc gactctcttt tctgctggcg gttgccacgc tgaacggcga gcgtctggat
    14221 ggtgagatga gtgaaggcga gctggtggat gcatttcgcc atgtgagtga tgcgtttgag
    14281 caaaccagcg aaaccatcgg cgtgcgcgcc aacaacgcga tcaacgacat ggtgcgtcaa
    14341 cgtctgctga accgctttac cagcgagcag gcggaaggga acgcaattta ccgtctgacg
    14401 ccgctcggca tcggcatcac tgactattac attcgtcagc gcgagttttc tacgctgcgc
    14461 ctttctatgc aattgtcgat tgtggcgggt gagctcaagc gcgcagcaga tgccgccgaa
    14521 gagggcggtg atgaatttca ctggcaccgt aatgtttatg cgccactgaa atattcggta
    14581 gcagaaattt tcgacagtat cgacctgacg caacgtctga tggatgaaca gcagcagcag
    14641 gtgaaggatg atatcgccca gttgttgaac aaagactggc gtgcggcgat ttccagctgt
    14701 gaattgttgc tttcagaaac ttccggaacg ctgcgtgaat tgcaggatac gctggaagcg
    14761 gcaggcgata aattgcaggc caacctgtta cgcattcagg atgcgacgat gacccatgac
    14821 gacctgcatt ttgtcgatcg tctggtgttc gatcttcaga gcaaactcga ccgtattatc
    14881 agttggggcc agcaatccat cgacttgtgg attggctacg accgccacgt acacaaattt
    14941 attcgtactg cgatcgatat ggataaaaac cgcgtctttg ctcagcggtt acgtcagtcg
    15001 gtacaaacct attttgatga gccgtgggcg ctaacttatg ccaatgccga tcgtctgctg
    15061 gatatgcgtg acgaagagat ggtactgcgc gatgaagaag tgactgggga acttcctgag
    15121 gatctggagt acgaagagtt taacgagatc cgcgaacagc tggcggcgat catcgaagaa
    15181 caacttgccg tgtacaaaac cagacaagtg ccgttggatc ttggtctggt ggtacgcgaa
    15241 tatctgtcac agtatccgcg tgcacgtcac tttgacgttg cgcgcattgt tattgatcag
    15301 gcggtacgtc ttggcgtagc gcaagcagat ttcaccggac tgccagcgaa atggcagccg
    15361 attaatgatt acggagccaa ggtacaggcg catgtcatcg acaaatattg aacaagtgat
    15421 gccggttaag ctggcgcagg cgctggcgaa tccgttattt ccggcgctgg atagcgcctt
    15481 acgttcaggc cgtcatattg gcctcgacga actggataat catgcattcc tgatggattt
    15541 tcaggaatat ctggaagagt tttacgcgcg ttataacgtt gagcttattc gtgcaccgga
    15601 agggttcttc tatctacgcc cacgttccac cacgctgatc cctcgttccg tcttgtcgga
    15661 actggatatg atggtcggga aaatcctctg ttatctctat ctcagcccgg aacggctggc
    15721 gaatgagggg atttttactc aacaggaact gtacgacgaa ctgctcaccc tggccgatga
    15781 agcgaaactg ctgaaactgg tgaacaaccg ttcaaccggt tcagacgttg accgtcagaa
    15841 gttgcaggaa aaagtacgtt cttcgctcaa ccgtctgcgt cgtttaggca tggtgtggtt
    15901 tatgggccac gacagcagca agttccgcat taccgaatcg gtgttccgct tcggagccga
    15961 tgtgcgtgct ggcgacgatc cccgtgaagc acagcgtcgc ctgattcgtg atggtgaagc
    16021 aatgccgatt gaaaatcatc tgcaactcaa cgatgaaacc gaagagagtc agccagatag
    16081 cggagaggaa gaataatgat tgaacgcggt aaatttcgct cactgacgct gattaactgg
    16141 aacggctttt ttgcccgaac ttttgacctt gacgagctgg tcacgacgct ttctggcggt
    16201 aacggggcgg gtaaatccac caccatggcg gcgttcgtta cggcgcttat ccccgatctg
    16261 accctgctgc atttccgtaa cactacggaa gccggggcca ccagcggttc gcgcgataaa
    16321 ggtctgcacg gtaagctgaa agcgggtgtc tgttattcga tgctcgacac cattaactcg
    16381 cgccaccagc gtgtagtggt cggtgtgcgt ctgcaacagg ttgccggacg cgatcgtaaa
    16441 gtggatatca aaccatttgc cattcagggc ctgccgatgt cggtgcagcc gacacaactg
    16501 gtgaccgaaa ctctgaacga acgccaggcg cgcgtgctgc cgcttaatga gctgaaagac
    16561 aagctcgagg cgatggaagg cgtgcagttt aagcagttca actccattac cgattaccac
    16621 tcgctgatgt tcgatctggg catcatcgcg cgtcgtctgc gctcagcgtc tgaccgtagt
    16681 aaattctatc gtctgatcga agcctcgctg tatggcggga tctccagcgc cattactcgt
    16741 tctctgcgcg actacctgtt gccggaaaac agcggggtgc ggaaagcgtt ccaggacatg
    16801 gaagcggcgc tgcgtgaaaa ccgcatgacg ctggaagcga ttcgtgtcac ccagtcggac
    16861 cgtgacctgt ttaagcatct gatcagcgaa gccaccaact acgtggcggc ggactacatg
    16921 cgtcacgcca acgagcgccg tgttcatctc gataaggccc tggagtttcg tcgcgagcta
    16981 catacttcgc gtaagcaact ggcggctgag cagtacaaac acgtcgacat ggcgcgagag
    17041 ctggctgagc acaacggtgc cgaaggcgat ctggaagcgg attatcaggc ggccagcgat
    17101 cacctgaacc tggtgcaaac cgcactgcgt cagcaggaga agatcgaacg ctacgaagcg
    17161 gatctcgatg agctgcagat ccgtctggaa gagcaaaacg aagtggtggc agaagccatc
    17221 gaacgccagg aagagaacga ggctcgtgcg gaagctgccg aactggaagt ggacgagctg
    17281 aaaagccagc ttgctgacta ccagcaggcg ctggacgtcc agcaaacgcg cgcgatccag
    17341 tataaccagg cgattgctgc gcttaatcgt gccaaagaac tgtgccatct gccggactta
    17401 accgccgaca gcgctgccga atggctggaa accttccagg cgaaagagct ggaagcgacc
    17461 gaaaaaatgc tctctcttga gcagaaaatg agcatggcgc aaaccgcgca cagccagttt
    17521 gagcaggctt atcagctggt ggtggcaatc aacggcccgc tggcgcgtaa cgaggcgtgg
    17581 gatgtcgctc gcgaactatt gcgcgaaggg gtcgatcagc gtcacctggc agagcaggtt
    17641 cagccgttgc ggatgcgctt aagcgaactg gaacagcgtc tgcgtgagca gcaagaagct
    17701 gagcgtctgc tggcagattt ctgcaaacgt cagggcaaga attttgatat tgatgaactg
    17761 gaagctctgc atcaggagct ggaagcacgc attgcctctc tttccgatag cgtctctaac
    17821 gcccgagaag agcggatggc actgcgccag gagcaggaac agctgcagtc tcgcattcag
    17881 agtttgatgc agcgtgcgcc ggtttggctg gcagcgcaaa acagtctcaa ccagttgagc
    17941 gaacagtgcg gcgaagagtt cacctccagc caggatgtca ccgaatttct gcaacagttg
    18001 ctggagcgtg agcgcgaggc aattgttgaa cgcgatgaag taggcgcgcg caaaaacgcc
    18061 gtcgatgaag agatcgaacg tttaagccag ccaggtggtt ctgaagatca gcgtctgaac
    18121 gcgctggcgg agcgttttgg tggtgtgctg ctgtcagaaa tttatgacga cgttagcctg
    18181 gaagatgcgc cgtacttctc agcgctgtat ggcccgtcac gccacgccat cgtggtgcca
    18241 gatctgtcac aggtaaccga acacctggaa ggcttgaccg attgcccgga agatctctat
    18301 ctgatcgaag gggatccgca gtcattcgat gacagcgtgt tcagcgttga tgagctggaa
    18361 aaagcggtgg tggtgaaaat cgccgatcgt cagtggcgtt attcacgttt cccggaagtg
    18421 ccgctgtttg gtcgtgctgc gcgtgaaagc cgtattgaaa gcctccatgc cgagcgtgaa
    18481 gtgctttccg aacgcttcgc cacgctctcc tttgatgtac agaaaaccca gcgtctgcat
    18541 caggcgttca gccgctttat cggcagtcat ctggcggtgg cgtttgagtc tgacccggaa
    18601 gcagaaatcc gtcaactgaa cagccgtcgc gtcgaactgg aacgggcgtt gagtaatcat
    18661 gaaaatgata accagcagca gcgtattcag tttgagcagg cgaaagaggg cgttacggcg
    18721 ctgaaccgca ttctgccgcg tctcaacctg ttggctgatg acagcctggc ggatcgcgtc
    18781 gatgaaatcc gcgaacgtct ggatgaagcc caggaagccg cgcgttttgt tcagcagttt
    18841 ggcaatcaac tggcgaaact ggaaccgatc gtttcggtat tgcagagcga cccggaacag
    18901 ttcgaacagt taaaagaaga ttacgcgtac tctcagcaga tgcagcgcga tgcccgtcag
    18961 caggcgtttg ccctgacgga agtggtgcag cgtcgcgcgc actttagcta ctctgactcg
    19021 gcagaaatgc ttagcggtaa cagcgatctc aacgaaaaac tgcgtgaacg tctggaacag
    19081 gcggaagcgg agcgtacccg cgctcgtgaa gcgttgcgcg gacacgcagc gcagttgaat
    19141 cagtacaacc aggtgctggc ttcgctgaaa agttcgtatg acaccaaaaa agagctactt
    19201 aacgatctgc aacgtgaatt gcaggatatc ggcgtgcgtg ctgatagcgg ggcagaagag
    19261 cgggcacgta ttcgccgtga cgagttgcac gcgcaactga gcaataaccg ttcacgacgc
    19321 aatcaactgg aaaaagcgct taccttctgc gaagcggaga tggacaacct gacccgtaaa
    19381 ctgcgcaagc tggagcggga ttactttgag atgcgcgagc aggtagtgac cgccaaagcg
    19441 ggctggtgtg cggtgatgcg catggtgaaa gataatggcg ttgagcgtcg cttacaccgt
    19501 cgtgagctgg cttatctctc cgctgatgat ttgcgttcca tgtcggataa ggcgttaggt
    19561 gcgctgcgtc tggcggtggc agataacgaa catctgcgcg acgtgctgcg gatgtcggaa
    19621 gatccaaaac gtccggaacg taaaattcag ttcttcgtgg cggtttatca gcatctgcgt
    19681 gaacgtattc gtcaggatat tattcgtacc gatgatccgg tggaagctat cgaacagatg
    19741 gagatcgagc ttagccgtct gaccgaagaa ttaacctccc gtgaacagaa actggcgatc
    19801 agttcccgca gcgtggcgaa catcattcgc aaaaccattc agcgcgagca gaaccgtatc
    19861 cgtatgctca accaggggtt gcagaacgta tcgtttggcc aggtgaacag cgtacgtctg
    19921 aacgtgaacg tacgtgaaac gcacgccatg ctgctggatg tgctctctga acagcacgag
    19981 cagcatcagg atctgtttaa cagcaaccgc ctgaccttct cagaagcgct ggcgaaactg
    20041 tatcaacgtc ttaacccgca gattgatatg gggcagcgca cgccgcagac catcggtgaa
    20101 gaactgctgg attaccgcaa ctatctggaa atggaagttg aggttaaccg tggttccgat
    20161 ggctggttgc gtgcagagtc tggtgcattg tcgaccggtg aggcgattgg taccggtatg
    20221 tcgattctgg tgatggtggt acaaagctgg gaagatgaat ctcgccgcct gcgcggtaaa
    20281 gatatctctc cttgccgcct gctgttcctc gatgaagcag cgcgactgga cgcccgttcc
    20341 atcgccacgc tgtttgaatt gtgtgaacgt ttgcaaatgc agctcattat cgcagcgccg
    20401 gaaaatatca gcccggagaa aggcaccacc tataaactgg tgcgtaaagt cttccagaat
    20461 accgaacacg ttcacgtcgt cggcctgcga ggatttgcgc ctcaactccc tgaaacgctt
    20521 ccaggaagtg acgaagcgcc ttctcaggcg agttaaagaa aaggcggcag caatgccgcc
    20581 ttttcttttt ccgaaaactc cgtttctgca ctaaaaaagt ggcacattac ggcgcgcttt
    20641 tctttaaact tctttacatt aggttatgta aaaacgtatc ggcgtttata tactgaagat
    20701 aagtctgatg agtaacaggc ttgctcgtca tactttcgtg agtattggcg ttgtacaggc
    20761 aagtcgtaaa ataacagcct ggctattcag agtatgataa aaacaggggg caagggatgt
    20821 tgcttaatat gatgtgtggt cgtcggctgt cggcaatcag tttgtgcctg gccgtaacat
    20881 tcgctccact gttcaatgcg caggccgatg agcctgaagt aatccctggc gacagcccgg
    20941 tggctgtcag tgaacagggc gaggcactgc cgcaggcgca agccacggca ataatggcgg
    21001 ggatccagcc attgcctgaa ggtgcggctg aaaaagcccg cacgcaaatc gaatctcaat
    21061 tacccgcagg ttataagccg gtttatctta accagcttca attgttgtat gccgcacgcg
    21121 atatgcaacc catgtgggaa aaccgtgatg ctgttaaagc cttccagcaa cagctggcag
    21181 aggtggcgat tgccggtttc cagccgcagt ttaataaatg ggtagagtta ctgaccgatc
    21241 ctggtgttaa cgggatgaca cgcgacgtgg tgctctctga tgcgatgatg ggctatctcc
    21301 atttcattgc aaatattccg gtcaaaggca ctcgctggct atatagcagt aaaccttatg
    21361 cgcttgcaac gccgccgctt tcggtgatta accaatggca gctggcgctg gataaaggtc
    21421 aattgccgac gtttgttgca ggactggcac cgcagcatcc gcaatatgcg gcgatgcatg
    21481 aatcgttact ggccttactc agtgacacca aaccgtggcc tcaactgacc ggtaaagcaa
    21541 cgttgcgccc aggacagtgg agtaacgacg taccggcgtt gcgcgaaata ttgcaacgta
    21601 caggcatgtt ggacggggga ccgaaaatta cgctacctgg cgatgacacg ccaactgacg
    21661 cggtagtcag cccatccgct gttactgttg aaacagcaga aactaagctg atggacaaac
    21721 aaactacgtc tcgtagtaaa cctgcgcctg ccgttcgcgc cgcctacgat aatgaactgg
    21781 tggaagccgt taaacgtttt caggcatggc aaggattggg agcagatggt gctattggcc
    21841 cggcaacgcg tgactggtta aacgtaacgc ccgcccagcg tgctggcgtg ttggcactca
    21901 acatccagcg attgcgcttg ctgccaacag agctttctac cgggatcatg gttaacattc
    21961 cagcctattc gctggtctac tatcagaacg gcaatcaggt gctggattcg cgagtcattg
    22021 tcggtcgccc cgatcgcaaa acgccgatga tgagcagtgc acttaacaac gtagtggtaa
    22081 acccgccgtg gaacgtacca ccaactctgg cacgcgaaga tattctgccg aaagtgcgca
    22141 acgatccggg atatctcgaa agccatggct atacggtgat gcgcggctgg aacagcagag
    22201 aagcgattga cccatggcag gttgactggt ctacaatcac ggcctcgaat ttaccgttcc
    22261 gcttccagca ggctccaggc ccacggaact cgctggggcg ctataaattc aatatgccga
    22321 gttcagaggc catttatttg catgacacgc cgaaccacaa tctgttcaag cgtgatacac
    22381 gcgcattgag ctcaggctgt gtacgagtga ataaagcttc cgatctggcg aatatgctgt
    22441 tgcaggatgc aggctggaat gacaaacgta tttctgatgc gctgaagcag ggcgatacgc
    22501 gttacgtcaa tattcggcag tcgattccgg tgaatctcta ctatctgacg gcctttgttg
    22561 gtgcagatgg tcgtacccag tatcgtacag atatttacaa ttatgatctg cctgcgcgat
    22621 ccagctcgca aatcgtatcg aaagcggaac aattaatcag gtaaatgaag cagttcgggg
    22681 aaatcagttg tcgtaataat tagcatgatc gggggcgatt ctctgcagcc cccgtcactg
    22741 ctggggttga gtcatcttga cgtctgcttt acgggcggtt aaggtgcctc ttgtgcgcca
    22801 gaagtgcata taaacgataa cattgacctg tagacttgat tatcatggac aaattcgacg
    22861 ctaatcgccg caaactgctg gcgcttggtg gcgttgcact cggtgccgcc atcctgccga
    22921 ccccggcgtt tgcaacactc tctaccccac gcccgcgcat tttgacactc aataatcttc
    22981 ataccggaga gtcaatcaaa gcggagtttt tcgatggcag aggctatatt caggaagaat
    23041 tggcaaaact taaccatttt ttccgcgatt accgcgcgaa caaaataaag tccatcgacc
    23101 caggattatt cgaccagttg tatcgcctgc aagggttgtt aggcacccgc aaaccggtgc
    23161 aactcatttc cggttatcgt tccattgata ccaacaatga actacgcgcc cgcagccgtg
    23221 gagtagcgaa gaaaagctat cacactaaag gccaagcgat ggatttccac attgaaggta
    23281 tcgcgttaag caatattcgc aaagccgcgt tatctatgcg cgcaggtggt gtaggatatt
    23341 acccacgtag taactttgtg catattgata ccgggccagc acggcactgg tagtaatcgc
    23401 ttaacgaaac aggggcagta tgaactatcg tattattccg gtcaccgcat tctcccagaa
    23461 ctgttcattg atctggtgtg aacaaacccg tctggccgca ctggtcgatc ctggcggcga
    23521 tgcggaaaaa atcaaacagg aagttgatgc cagcggcctg acactgatgc agatcctgct
    23581 gactcatggt catctggacc acgttggcgc agcggcggaa ctggcgcaac attacggcgt
    23641 gccggttttt ggcccggaaa aagaagatga gttctggctg caaggcttgc ctgcgcaaag
    23701 tcgtatgttt ggtctggaag agtgccagcc gctgacgcca gatcgttggc tgaacgaagg
    23761 cgataccatc agcataggga atgtgacttt acaggtgtta cattgccctg ggcatacgcc
    23821 gggtcatgtc gtgttttttg atgatcgggc aaagctgctg atttctggcg acgttatttt
    23881 caaaggtgga gtagggcgca gtgacttccc gcgtggcgat cataatcaac tgatttcttc
    23941 aatcaaagat aaattgctgc cactggggga tgacgtgaca tttattccgg gtcacggacc
    24001 attatccaca cttggttatg agcgcctgca taatcccttc ctgcaagacg aaatgcccgt
    24061 ctggtaaagg cgcataaaaa agcccgcttt caagcgggct tcatcggttt taacgcttac
    24121 agcactgcca caatcgcttc gcacagcggc gccatgttat ctggtgtcat cccggccacg
    24181 tttacgcgac cagaagcaac agcatatacg ccaaactctt cgcgcagacg cagcacttgt
    24241 tcttttgtca ggccactgaa ggagaacatg ccgttctgtt tgatgataaa gctgaagtcg
    24301 cgatttgcgc ctttttcctg cagcgtattg acgaacaact gacgcatacg ctgaatacgc
    24361 tggcgcatat cagtcagttc ttgttcccaa atcgcacgta acgcatcgtt gctcaggatg
    24421 gtggcaacaa cagaagcgcc gtgtgctggt gggttagagt agttagcacg aatcgccgct
    24481 ttcatttggc tgaatgcgcg ttcaacagtt tcactgtcag cagcaaccag agtacaagcg
    24541 ccaacacgct cgttgtacag gccaaagttt ttagagtagg aactggcaac aatcagctcc
    24601 ttatgcagag ccgcgaaagc gcgcagtcct tcagcatctt cttccagacc acgggcaaaa
    24661 ccctggtaag cgaagtcaaa cagcggtaac cagcctttct caacggagag ttgtgccagt
    24721 gtctgccatt gttccagcgt agggtcgatg ccggttgggt tatggcagca gccatggaac
    24781 agcactacgt cgccagcctg ggcttcgttc aggctgttaa tcagtgcatc gaagtcaaga
    24841 gtgtgatttt ccgcatcata ataagcgtat tcacgaactt ccagacctgc agagttaaag
    24901 acgctcttat ggttcggcca gcttgggttg ctcacccaca cacgcttaac gctggtattt
    24961 tttgccagga aatcggcagc cacgcgtagt gcgccagtgc cacccggagt ctgtgccgtg
    25021 cgagcacgtt tgtcattgat cagggcgcta cctttaccaa acagcagttc ctgagtgcag
    25081 cgaccaaatt cagggatgcc gtcaatgccg aggtaatttt tggtggtttc attttcgagc
    25141 agatactgtt cagccttttt cacgctggtc agtaccgggg ttttacccgt ctcatcttta
    25201 tagacaccaa tcccgaggtt aattttgccg ggacgttcat cggcacgaaa cagatcggcc
    25261 aggcccagaa tcgggtcggc aggagcggcg gtaatgttct caaacatgac gaggttccat
    25321 tatggttaca gaagggaagt ccgctatcag ggtaacggga gatttacaaa attccaacta
    25381 ttactgatga aaacgcaggc tgtttttgca agacgtgaga ttgctctgga aggcataaaa
    25441 aaaacaggac caaagtcctg ttttttcggc atttaacaaa gaggtgtgct attagaactg
    25501 gtaaacgata cccacagcaa cggtgtcgtc agaacctacg cccagtttgt tgtcagaatc
    25561 gatctggttg atgatgtagt caacataggt ggacatgttt ttgttgaaat agtaggttgc
    25621 gcccacttca aagtagttca ccagatcaac atcaccgata ccttctacgt ctttcgcttt
    25681 agatttggtg taagcgatgg acggacgcag accgaaatca aactggtatt gcgcaactaa
    25741 cagaacgtct tgcgttttgt tggcgaagcc gctggtgttt gtaaatttat tagtgatcgg
    25801 cgtagcgtta cgggtttcac cgtagttcgc tgccaggtag atgttgttcg cgtcatactt
    25861 cagaccagta gcccactgtt cagctttttt gcctttgcca agggaagact cttcctgcag
    25921 gttggtacgg tcagcggcac cataagcacc aacgatacca aagccttcgt attcgtagct
    25981 gatagaaccg ccaacaccgt cgccgttaga gcggcgtgca gtgtcacgct cgtttttacc
    26041 caggtactga acagcgaagt tcagaccatc aaccagacca aagaagttgg agttacgata
    26101 ggtagcaacg ccgccaacac gaccaacgaa gaaatcatcg ctgtatgcag tatcaccacc
    26161 aaattctggc agcatatcgg tgtaacccag tgcatcataa accacaccgt agttacggcc
    26221 gtaatcgaaa gaaccaacgt cagcgtattt aagacccgcg aatgccagac gcgttttgtt
    26281 accagtttga gcgtcagcgc cctcagagtt gttaccctgg aagttatatt cccactgacc
    26341 ataaccggtc agatcggaat tgatttgagt ttccccttta aaaccaagac gggcataggt
    26401 catgtcgcca ttgccaccgt aactgttttc accgttaccc ttggagaaat aatgcagacc
    26461 gacagcttta ccgtacagat ctactttgtt gccatctttg ttatagattt ctgcagcgtt
    26521 tgcagtacct gctactaaca gagcagggac gatcactgcc agaatattgc gcttcatcat
    26581 tatttattac cctcatggtt ttttttatga cacctgccac tgccgtcaat aagttctgtc
    26641 aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcaggcatct
    26701 ttccattcaa actaacgttt cgctaccgt
//
LOCUS       DADSZD010000053        26505 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.053, whole genome shotgun sequence.
ACCESSION   DADSZD010000053 DADSZD010000000
VERSION     DADSZD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.053"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            83..1552
                     /gene="gspA"
                     /locus_tag="J1Z28_001947"
     CDS             83..1552
                     /gene="gspA"
                     /locus_tag="J1Z28_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBA7716784.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETISLLQQAEQTRVFT
                     AKQWRMIYRVLGDKRPRKMQLAVVMSGTILALTCGWLLLSSFTAPLPVPAWLIPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTL
                     VDQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLW
                     KMSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLK
                     TDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            1554..1973
                     /gene="gspB"
                     /locus_tag="J1Z28_001948"
     CDS             1554..1973
                     /gene="gspB"
                     /locus_tag="J1Z28_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBA7716785.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            2211..2522
                     /gene="rpsJ"
                     /locus_tag="J1Z28_001949"
     CDS             2211..2522
                     /gene="rpsJ"
                     /locus_tag="J1Z28_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBA7716786.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2555..3184
                     /gene="rplC"
                     /locus_tag="J1Z28_001950"
     CDS             2555..3184
                     /gene="rplC"
                     /locus_tag="J1Z28_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBA7716787.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3195..3800
                     /gene="rplD"
                     /locus_tag="J1Z28_001951"
     CDS             3195..3800
                     /gene="rplD"
                     /locus_tag="J1Z28_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBA7716788.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3797..4099
                     /gene="rplW"
                     /locus_tag="J1Z28_001952"
     CDS             3797..4099
                     /gene="rplW"
                     /locus_tag="J1Z28_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBA7716789.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4117..4938
                     /gene="rplB"
                     /locus_tag="J1Z28_001953"
     CDS             4117..4938
                     /gene="rplB"
                     /locus_tag="J1Z28_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBA7716790.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4955..5233
                     /gene="rpsS"
                     /locus_tag="J1Z28_001954"
     CDS             4955..5233
                     /gene="rpsS"
                     /locus_tag="J1Z28_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBA7716791.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5248..5580
                     /gene="rplV"
                     /locus_tag="J1Z28_001955"
     CDS             5248..5580
                     /gene="rplV"
                     /locus_tag="J1Z28_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBA7716792.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5598..6299
                     /gene="rpsC"
                     /locus_tag="J1Z28_001956"
     CDS             5598..6299
                     /gene="rpsC"
                     /locus_tag="J1Z28_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBA7716793.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6312..6722
                     /gene="rplP"
                     /locus_tag="J1Z28_001957"
     CDS             6312..6722
                     /gene="rplP"
                     /locus_tag="J1Z28_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBA7716794.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6722..6913
                     /gene="rpmC"
                     /locus_tag="J1Z28_001958"
     CDS             6722..6913
                     /gene="rpmC"
                     /locus_tag="J1Z28_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBA7716795.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6913..7167
                     /gene="rpsQ"
                     /locus_tag="J1Z28_001959"
     CDS             6913..7167
                     /gene="rpsQ"
                     /locus_tag="J1Z28_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBA7716796.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7332..7703
                     /gene="rplN"
                     /locus_tag="J1Z28_001960"
     CDS             7332..7703
                     /gene="rplN"
                     /locus_tag="J1Z28_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013975775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBA7716797.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7714..8028
                     /gene="rplX"
                     /locus_tag="J1Z28_001961"
     CDS             7714..8028
                     /gene="rplX"
                     /locus_tag="J1Z28_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBA7716798.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8043..8582
                     /gene="rplE"
                     /locus_tag="J1Z28_001962"
     CDS             8043..8582
                     /gene="rplE"
                     /locus_tag="J1Z28_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBA7716799.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8597..8902
                     /gene="rpsN"
                     /locus_tag="J1Z28_001963"
     CDS             8597..8902
                     /gene="rpsN"
                     /locus_tag="J1Z28_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBA7716800.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8936..9328
                     /gene="rpsH"
                     /locus_tag="J1Z28_001964"
     CDS             8936..9328
                     /gene="rpsH"
                     /locus_tag="J1Z28_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBA7716801.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9341..9874
                     /gene="rplF"
                     /locus_tag="J1Z28_001965"
     CDS             9341..9874
                     /gene="rplF"
                     /locus_tag="J1Z28_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBA7716802.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9884..10237
                     /gene="rplR"
                     /locus_tag="J1Z28_001966"
     CDS             9884..10237
                     /gene="rplR"
                     /locus_tag="J1Z28_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBA7716803.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10252..10755
                     /gene="rpsE"
                     /locus_tag="J1Z28_001967"
     CDS             10252..10755
                     /gene="rpsE"
                     /locus_tag="J1Z28_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBA7716804.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10759..10938
                     /gene="rpmD"
                     /locus_tag="J1Z28_001968"
     CDS             10759..10938
                     /gene="rpmD"
                     /locus_tag="J1Z28_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBA7716805.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10942..11376
                     /gene="rplO"
                     /locus_tag="J1Z28_001969"
     CDS             10942..11376
                     /gene="rplO"
                     /locus_tag="J1Z28_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBA7716806.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11384..12715
                     /gene="secY"
                     /locus_tag="J1Z28_001970"
     CDS             11384..12715
                     /gene="secY"
                     /locus_tag="J1Z28_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBA7716807.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12747..12863
                     /gene="rpmJ"
                     /locus_tag="J1Z28_001971"
     CDS             12747..12863
                     /gene="rpmJ"
                     /locus_tag="J1Z28_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBA7716808.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13010..13366
                     /gene="rpsM"
                     /locus_tag="J1Z28_001972"
     CDS             13010..13366
                     /gene="rpsM"
                     /locus_tag="J1Z28_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBA7716809.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13383..13772
                     /gene="rpsK"
                     /locus_tag="J1Z28_001973"
     CDS             13383..13772
                     /gene="rpsK"
                     /locus_tag="J1Z28_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBA7716810.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13806..14426
                     /gene="rpsD"
                     /locus_tag="J1Z28_001974"
     CDS             13806..14426
                     /gene="rpsD"
                     /locus_tag="J1Z28_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBA7716811.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14452..15441
                     /gene="rpoA"
                     /locus_tag="J1Z28_001975"
     CDS             14452..15441
                     /gene="rpoA"
                     /locus_tag="J1Z28_001975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBA7716812.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15482..15865
                     /gene="rplQ"
                     /locus_tag="J1Z28_001976"
     CDS             15482..15865
                     /gene="rplQ"
                     /locus_tag="J1Z28_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBA7716813.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15971..16339
                     /locus_tag="J1Z28_001977"
     CDS             15971..16339
                     /locus_tag="J1Z28_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBA7716814.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEHRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16350..16775
                     /gene="zntR"
                     /locus_tag="J1Z28_001978"
     CDS             16350..16775
                     /gene="zntR"
                     /locus_tag="J1Z28_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBA7716815.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16831..17049
                     /locus_tag="J1Z28_001979"
     CDS             16831..17049
                     /locus_tag="J1Z28_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HBA7716816.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(17046..17459)
                     /gene="mscL"
                     /locus_tag="J1Z28_001980"
     CDS             complement(17046..17459)
                     /gene="mscL"
                     /locus_tag="J1Z28_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBA7716817.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17589..18965)
                     /gene="trkA"
                     /locus_tag="J1Z28_001981"
     CDS             complement(17589..18965)
                     /gene="trkA"
                     /locus_tag="J1Z28_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBA7716818.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18987..20276)
                     /gene="rsmB"
                     /locus_tag="J1Z28_001982"
     CDS             complement(18987..20276)
                     /gene="rsmB"
                     /locus_tag="J1Z28_001982"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBA7716819.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQSDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDKAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSQQIKAFLQR
                     TEDAMLCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20328..21275)
                     /gene="fmt"
                     /locus_tag="J1Z28_001983"
     CDS             complement(20328..21275)
                     /gene="fmt"
                     /locus_tag="J1Z28_001983"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBA7716820.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLTCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            complement(21290..21799)
                     /gene="def"
                     /locus_tag="J1Z28_001984"
     CDS             complement(21290..21799)
                     /gene="def"
                     /locus_tag="J1Z28_001984"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBA7716821.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21929..23053
                     /gene="dprA"
                     /locus_tag="J1Z28_001985"
     CDS             21929..23053
                     /gene="dprA"
                     /locus_tag="J1Z28_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBA7716822.1"
                     /translation="MFDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKATLQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            23025..23498
                     /locus_tag="J1Z28_001986"
     CDS             23025..23498
                     /locus_tag="J1Z28_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBA7716823.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23527..24069
                     /locus_tag="J1Z28_001987"
     CDS             23527..24069
                     /locus_tag="J1Z28_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716824.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            24074..24646
                     /gene="tsaC"
                     /locus_tag="J1Z28_001988"
     CDS             24074..24646
                     /gene="tsaC"
                     /locus_tag="J1Z28_001988"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBA7716825.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKRRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24651..25469
                     /gene="aroE"
                     /locus_tag="J1Z28_001989"
     CDS             24651..25469
                     /gene="aroE"
                     /locus_tag="J1Z28_001989"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBA7716826.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNTFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGCLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            25466..25723
                     /locus_tag="J1Z28_001990"
     CDS             25466..25723
                     /locus_tag="J1Z28_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBA7716827.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25699..26253)
                     /locus_tag="J1Z28_001991"
     CDS             complement(25699..26253)
                     /locus_tag="J1Z28_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBA7716828.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6772 a         6230 c         6907 g         6596 t
ORIGIN      
        1 tattagtaat atgtatttaa ttaataaata catacattat ttttatgatt aaggataatc
       61 aataattaaa acaggaagtt ctatgtctac gagaagagaa gtcattcttt cctggttgcg
      121 tgagaaacgt cagacctggc gtctgtgcta tttgttgggt gaggctggaa gtggaaaaac
      181 ctggctggcg cagcaactac aaaaagataa acatcgccgt gtgattactt taagcctcgt
      241 tgtttcctgg caaggtaagg ccgcatggat cgttaccgac gataacgcgg ctgaacaggg
      301 ctgccgtgac agcgcctgga cgcgagatga gatggcgggt caattactgc atgcacttca
      361 tcgaactgat agtcgctgcc ctcttattat tattgaaaat gctcacctga atcaccgccg
      421 catacttgat gatctccaac gcgcgatatc tcttattcct gacggtcaat ttttgctgat
      481 cggcagaccc gatcgaaaag ttgaacgtga ttttaaaaaa cagggcattg aacttgtctc
      541 gacaggtcgt ctgacggagc acgagcttaa ggcaagcatc cttgaaggtc agaatattga
      601 ccagcccgat ctccttttaa ccgccagagt tctgaaacgg atagctttat tatgtcgggg
      661 agatcgcaga aagctggcgc ttgccggcga aacaattagc ttattacagc aagccgagca
      721 gacccgcgtt ttcactgcaa aacaatggcg aatgatttac cgcgtattag gtgataagcg
      781 cccccggaaa atgcagcttg ccgtggtgat gagtggaacc attctcgcac ttacctgtgg
      841 ttggctactg ctgtcctctt tcacagctcc gttacccgtt cctgcgtggc tgatacccgt
      901 aacgccagta gttaaacaag atatgactaa ggatattgct catgtggtga tgcgcgatag
      961 tgaggcgcta agcgtgttat acggcgtatg ggggtacgag gttccagccg attcggcctg
     1021 gtgcgatcag gcagtaagag cggggctggt atgtaaaagt gggaacgctt ctttacaaac
     1081 attggtagat cagaatttgc cctggatcgc atcgcttaaa gtcggggaca aaaaattgcc
     1141 tgttgtggtc gttcgcgtcg gtgatgctac cgttgatgta ctcgtagggc agcaaacctg
     1201 gacgctgaca cataaatggt ttgagttggt atggacgggt gattatcttc tgttgtggaa
     1261 aatgtcgccg gaaggggaga gtaccattac gcgtgatagc agcgaggaag agattctctg
     1321 gctggaaacg atgttaaacc gcgcattgca tatttcgaca gaatcttcgg cagagtggcg
     1381 tcccttgttg gtagaaaaaa taaagcaatt tcaaaaaagc catcacttga aaactgacgg
     1441 tgttgttggc ttttcaacgt tagtacatct ctggcaggtg gcgggggaga gtgcctactt
     1501 atatcgggat gaagcaaata tttccccaga aacaacagtg aaggggaaat gacatgtttg
     1561 aattctatat cgccgcgcgt gagcagaaag aaacgggtca ccctggtatc ttctcccgac
     1621 aaaaacacag caccataata tatgtcattt gtttattgct gatttgcctg tggtttgcag
     1681 gaatggtgtt ggttggtggg tatgcccggc agctatgggc actttggata gtaaaagcag
     1741 aagtcactgt agatgctgaa acgcccgctt ttaaacaatc aacacaacac tattttttca
     1801 aaaaacagcc actccccgtc gtcgaatccg tcgaggagga agatgaacca ggcgtcgcgg
     1861 ttgagaatgc cccctcttct tctgaagacg aagaaaatac tgtagaagaa agcgacgaga
     1921 aagccggttt aagagagcgt gtcaaaaatg cgctgaacga acttgagaga taacccaaag
     1981 gctgtttact ttactagcaa tacgcttgcg ttcggtggtt aagtatgtat aatgcgcggg
     2041 cttgtcgtag ttgacagcag gttcaatctg aacctcagtt agccgaattt ggctacctaa
     2101 caatgctccc aatcggggag ctacgtaaga acggttacac tctcccatca atcgtaatgg
     2161 gtctgaggag taatcatttt cgtttataaa ataattggag ctctggtctc atgcagaacc
     2221 aaagaatccg tatccgcctg aaagcgtttg atcatcgtct gatcgatcaa gcaaccgcgg
     2281 aaatcgtcga gactgctaag cgcactggtg cgcaggtccg tggtccgatc ccgctgccga
     2341 cacgcaaaga gcgcttcact gttctgatct ccccgcacgt caacaaagac gcgcgcgatc
     2401 agtacgaaat ccgtactcac ttgcgtctgg ttgacatcgt tgagccaacc gagaaaaccg
     2461 ttgatgctct gatgcgtctg gatctggctg ccggtgtaga cgtgcagatc agcctgggtt
     2521 aatcaggtca ttgagcgatt gagaggttga aacaatgatt ggtttagtcg gtaaaaaagt
     2581 gggtatgacc cgtatcttca cagaagacgg cgtttctatc ccagtaaccg taatcgaagt
     2641 tgaagcaaac cgcgttactc aggttaaaga cctggctaac gatggctacc gtgctattca
     2701 ggtgaccacc ggtgctaaaa aagctaaccg tgtgaccaag cctgaagctg gccacttcgc
     2761 taaagctggc gtagaagctg gccgtggtct gtgggaattc cgcctggctg aaggcgaaga
     2821 gttcactgta ggtcagagca ttagcgttga actgtttgct gacgttaaaa aagttgacgt
     2881 aactggcacc tctaaaggta aaggtttcgc aggtaccgtt aagcgctgga acttccgtac
     2941 ccaggacgct actcacggta actccttgtc tcaccgcgtt ccgggttcta tcggtcagaa
     3001 ccagactccg ggcaaagtgt tcaaaggcaa gaaaatggca ggtcagatgg gtaacgaacg
     3061 tgtaaccgtt cagagccttg acgtagtacg cgttgacgct gagcgcaacc tgctgctggt
     3121 taaaggcgct gtcccgggtg caaccggtag cgacctgatc gttaaaccag ctgtgaaggc
     3181 gtaaggagat agcaatggaa ttagtattga aagacgcgca gagcgcgctg actgtttccg
     3241 aaactacctt cggtcgtgat ttcaacgaag cgctggttca ccaggttgtt gttgcttatg
     3301 cagctggtgc tcgtcagggt actcgtgctc agaagactcg tgctgaagta actggttccg
     3361 gtaaaaaacc gtggcgccag aaaggcaccg gccgtgcgcg ttctggttct atcaagagcc
     3421 cgatctggcg ttctggtggc gtgacctttg ctgctcgtcc gcaggaccac agtcaaaaag
     3481 ttaacaagaa gatgtaccgc ggcgcgctga aaagcatcct gtccgaactg gtacgtcagg
     3541 atcgtctgat cgttgtcgag aagttctctg tagaagcgcc gaaaactaag ctgctggcac
     3601 agaaactgaa agacatggct ctggaagatg tgctgatcat caccggtgag ctggatgaga
     3661 acctgttcct ggctgcgcgc aacctgcaca aggttgacgt acgcgatgca actggtatcg
     3721 acccggttag cctgatcgcc ttcgacaaag tcgtaatgac tgctgatgct gttaagcaag
     3781 ttgaggagat gctggcatga ttcgtgaaga acgtctgctg aaggtgctgc gtgcaccgca
     3841 cgtttctgaa aaagcgtcta ctgcgatgga aaaatccaac accatcgtac tcaaagttgc
     3901 taaagacgcg accaaagcag aaatcaaagc tgctgtgcag aaactgtttg aagtcgaagt
     3961 cgaagtcgtt aacaccctgg tagttaaagg gaaagttaaa cgtcacggac agcgtatcgg
     4021 tcgtcgtagc gactggaaaa aagcttacgt caccctgaaa gaaggccaga atctggactt
     4081 cgttggcggc gctgagtaag tcggaggagt aatacaatgg cagttgttaa atgtaaaccg
     4141 acatctccgg gtcgtcgcca cgtagttaaa gtggttaacc ctgagctgca caagggcaaa
     4201 ccttttgctc cgttgctgga aaaaaacagc aaatccggtg gtcgtaacaa caatggccgt
     4261 atcaccactc gtcatatcgg tggtggccac aagcaggctt accgtattgt tgacttcaaa
     4321 cgcaacaaag acggtatccc ggcagttgtt gaacgtcttg agtacgatcc gaaccgttcc
     4381 gcgaacatcg cgctggttct gtacaaagac ggtgaacgcc gttacatcct ggcccctaaa
     4441 ggcctgaaag ctggcgacca gattcagtct ggcgttgatg ctgcaatcaa accaggtaac
     4501 accctgccga tgcgcaacat cccggttggt tctactgttc ataacgtaga aatgaaacca
     4561 ggtaaaggcg gtcagctggc acgttccgct ggtacttacg ttcagatcgt tgctcgtgat
     4621 ggtgcttatg tcaccctgcg tctgcgttct ggtgaaatgc gtaaagtaga agcagactgc
     4681 cgtgcaactc tgggcgaagt tggcaatgct gagcatatgc tgcgcgttct gggtaaagca
     4741 ggtgctgcac gctggcgtgg tgttcgtccg accgttcgcg gtaccgcgat gaacccggta
     4801 gaccacccac atggtggtgg tgaaggtcgt aactttggta agcacccggt aactccgtgg
     4861 ggcgttcaga ccaaaggtaa gaagacccgc agcaacaagc gtactgataa attcatcgta
     4921 cgtcgccgta gcaaataatt ttagaggata agccatgcca cgttctctca agaaaggtcc
     4981 ttttattgac ctgcacttgc tgaagaaggt agagaaagcg gtggaaagcg gagacaagaa
     5041 gcccctgcgc acttggtccc gtcgttcaac gatctttcct aacatgatcg gtttgaccat
     5101 cgctgtccat aatggtcgtc agcacgttcc ggtatttgta accgacgaaa tggtcggtca
     5161 caaactgggt gaattcgcac cgactcgtac ttatcgcggc cacgctgctg ataaaaaagc
     5221 gaagaagaaa taaggtagga ggaagagatg gaaactatcg ctaaacatcg ccatgctcgt
     5281 tcttctgctc agaaggttcg ccttgttgct gacctgattc gcggtaagaa agtgtcgcag
     5341 gctctggata ttttgaccta caccaacaag aaagcggctg tactggtcaa gaaagttctg
     5401 gaatctgcca ttgctaacgc tgaacacaac gatggcgctg acattgacga tctgaaagtt
     5461 acgaaaattt tcgtagacga aggcccgagc atgaagcgca ttatgccgcg tgcaaaaggt
     5521 cgtgcagatc gcatcctgaa gcgcaccagc cacatcactg tggttgtgtc cgatcgctga
     5581 gactctggag actagcaatg ggtcagaaag tacatcctaa tggtattcgc ctgggtattg
     5641 taaaaccatg gaactctacc tggtttgcga acaccaaaga attcgctgac aacctggaca
     5701 gcgattttaa agtacgtcag tacctgacta aggaactggc taaagcgtcc gtatctcgta
     5761 tcgttatcga gcgtccggct aagagcatcc gtgtaaccat tcacactgct cgcccgggta
     5821 tcgttatcgg taaaaaaggt gaagacgtag aaaaactgcg taaggtcgta gcggacatcg
     5881 ctggcgttcc tgcacagatc aacatcgccg aagttcgtaa gcctgaactg gacgcaaaac
     5941 tggttgctga cagcatcact tctcagctgg aacgtcgcgt tatgttccgt cgtgctatga
     6001 agcgtgctgt acagaacgca atgcgtctgg gcgctaaagg tattaaagtt gaagttagcg
     6061 gccgtctggg cggcgcggaa atcgcacgta ccgaatggta ccgcgaaggt cgcgtaccgc
     6121 tgcacactct gcgtgctgac atcgactaca acacctctga agcgcacacc acttacggtg
     6181 taatcggcgt taaagtgtgg atcttcaaag gcgagatcct gggtggtatg gctgctgttg
     6241 aacaaccgga aaaaccggct gctcagccta aaaagcagca gcgtaaaggc cgtaaataag
     6301 gagcgtcgct gatgttacaa ccaaagcgta caaaattccg taaaatgcac aaaggccgta
     6361 accgcggtct ggcgcagggt acggatgtta gcttcggcag cttcggtctg aaagctgttg
     6421 gccgtggtcg tctgactgca cgtcagatcg aagcagcacg tcgtgctatg acccgtgcag
     6481 ttaagcgtca aggtaagatc tggatccgtg tgttcccgga caaaccgatc actgaaaagc
     6541 cgctggcagt gcgtatgggt aaaggtaaag gtaacgtgga gtattgggtt gccttgattc
     6601 agccgggtaa agtcctgtat gaaatggacg gtgttccgga agagctggcc cgtgaagcat
     6661 tcaagctggc agcagcgaaa ctgccgatta aaaccacctt tgtaactaag acggtgatgt
     6721 aatgaaagca aaagagctgc gtgagaagag cgttgaagag ctgaacaccg agctgctgaa
     6781 cctgctgcgt gagcagttca acctgcgtat gcaggctgca agtggccagc tgcaacagtc
     6841 tcacctgttg aagcaagtgc gtcgcgatgt cgcacgcgtt aagactttac tgaacgagaa
     6901 ggcgggtgcg taatgaccga taaaatccgt actctgcaag gtcgcgttgt tagcgacaaa
     6961 atggagaaat ccattgttgt tgctatcgaa cgttttgtga aacacccgat ctacggtaaa
     7021 ttcatcaagc gtacgaccaa actgcacgta catgacgaga acaacgaatg cggtatcggt
     7081 gacgtggttg aaatccgcga atgccgtccg ctgtccaaga ctaaatcctg gacgctggtt
     7141 cgcgttgtag agaaagcggt tctgtaatac agtacactct ctcgatacga ataaacggct
     7201 cagaaatgag ccgtttattt tttctaccca tatccttgaa gcggtgttat aatgccgcgc
     7261 cctcgatatg gggattttta acgacctgat tttcgggtct cagtagtagt tgacattagc
     7321 ggagcactaa aatgatccaa gaacagacta tgctgaacgt cgccgacaac tccggtgcac
     7381 gtcgcgtaat gtgtatcaag gttctgggtg gctcgcaccg tcgctacgca ggcgtaggcg
     7441 acatcatcaa gatcaccatc aaagaagcaa ttccgcgtgg taaggtcaaa aaaggtgatg
     7501 tgctgaaggc ggtagtggtg cgcaccaaga agggtgttcg tcgcccggac ggttctgtca
     7561 ttcgcttcga tggtaatgct tgtgttcttc tgaacaacaa cagcgagcag cctatcggta
     7621 cgcgtatttt tgggccggta actcgtgagc ttcgtagtga gaagttcatg aaaattatct
     7681 ctctggcacc agaagtactc taaggagcga atcatggcag cgaaaatccg tcgtgatgac
     7741 gaagttatcg tgttaaccgg taaagataaa ggtaaacgcg gtaaagttaa gaatgtcctg
     7801 tcttccggca aggtcattgt tgaaggtatc aacctggtta agaaacatca gaagccggtt
     7861 ccggccctga accaaccggg tggcatcgtt gaaaaagaag ccgctattca ggtttccaac
     7921 gtagcaatct tcaatgcgac aaccggcaag gctgaccgtg taggctttag attcgaagac
     7981 ggtaaaaaag tccgtttctt caagtctaac agcgaaacta tcaagtaatt tggagtagta
     8041 cgatggcgaa actgcatgat tactacaaag acgaagtagt taaaaaactc atgactgagt
     8101 ttaactacaa ttctgtcatg caagtccctc gggtcgagaa gatcaccctg aacatgggtg
     8161 ttggtgaagc gatcgctgac aaaaaactgc tggataacgc agcagcagac ctggcagcaa
     8221 tctccggtca aaaaccgctg atcaccaaag cacgcaaatc tgttgcaggc ttcaaaatcc
     8281 gtcagggcta tccgatcggc tgtaaagtaa ctctgcgtgg cgaacgcatg tgggagttct
     8341 ttgagcgcct gatcactatt gctgtacctc gtatccgtga cttccgtggc ctgtccgcta
     8401 agtctttcga cggtcgtggt aactacagca tgggtgtccg tgagcagatc atcttcccag
     8461 aaatcgacta cgataaagtc gaccgcgttc gtggtttgga tattaccatt accactactg
     8521 cgaaatctga cgaagaaggc cgcgctctgc tggctgcctt tgacttcccg ttccgcaagt
     8581 aaggtagggt tactaaatgg ctaagcaatc aatgaaagca cgcgaagtaa aacgcgtagc
     8641 tttagctgat aaatacttcg cgaaacgcgc tgaactgaaa gcgatcatct ctgatgtgaa
     8701 cgcttccgac gaagatcgtt ggaacgctgt tctcaagctg cagactctgc cgcgtgattc
     8761 cagcccgtct cgtcagcgta accgctgccg tcaaacaggt cgtccgcatg gtttcctgcg
     8821 gaagttcggg ttgagccgta ttaaggtccg tgaagccgct atgcgcggtg aaatcccggg
     8881 tctgaaaaag gctagctggt aattgtcacc aattgaatca cgggagtaaa gacagatgag
     8941 catgcaagat ccgatcgcgg atatgctgac ccgtatccgt aacggtcagg ccgcgaacaa
     9001 agctgcggtc accatgcctt cctccaagct gaaagtggca atcgccaacg tgctgaagga
     9061 agaaggtttt attgaagatt ttaaagttga aggcgacacc aagcctgaac tggaacttac
     9121 tctgaagtat ttccagggca aagctgttgt agaaagcatt cagcgtgtca gccgcccagg
     9181 tctgcgcatc tataaacgta aagatgagct gccgaaagtt atggcgggtc tgggtatcgc
     9241 agttgtttct acctctaaag gtgttatgac tgatcgtgca gcgcgccagg ctggtcttgg
     9301 tggcgaaatt atctgctacg tagcctaatc ggaggaaaaa atgtctcgtg ttgctaaagc
     9361 accggtcgtt gttcctgccg gcgttgacgt aaaaatcaac ggtcaggtta ttacgatcaa
     9421 aggtaaaaac ggcgagctga ctcgtactct caacgatgct gttgaagtta aacatgcaga
     9481 taataccctg accttcggtc cgcgtgatgg ttacgcagac ggttgggcac aggctggtac
     9541 cgcgcgtgcc ctgctgaact caatggttat cggtgttacc gaaggcttca ctaagaagct
     9601 gcagctggtt ggtgtaggtt accgtgcagc ggttaaaggc aatgtgatta acctgtctct
     9661 gggtttctct catcctgttg accatcagct gcctgcgggt atcactgctg aatgtccgac
     9721 tcagactgaa atcgtgctga aaggcgctga taagcaggtg atcggccagg ttgcagcgga
     9781 tctgcgcgcc taccgtcgtc ctgagcctta caaaggcaag ggtgttcgtt acgccgacga
     9841 agtcgtgcgt accaaagagg ctaagaagaa gtaaggtaac actatggata agaaatctgc
     9901 tcgtatccgt cgtgcgaccc gcgcacgccg caagctccag gagctgggcg caactcgcct
     9961 ggtggtacat cgtaccccgc gtcacattta cgcacaggta attgcaccga acggttctga
    10021 agttctggta gctgcttcta ctgtagaaaa agctatcgct gaacaactga agtacaccgg
    10081 taacaaagac gcggctgcag ctgtgggtaa agctgtcgct gaacgcgctc tggaaaaagg
    10141 catcaaagat gtatcctttg accgttccgg gttccaatat catggtcgtg tccaggcact
    10201 ggcagatgct gcccgtgaag ctggccttca gttctaaggt agaggtgtaa gatggctcac
    10261 atcgaaaaac aagctggcga actgcaggaa aagctgatcg cggtaaaccg cgtatctaaa
    10321 accgttaaag gtggtcgtat tttctccttc acagctctga ctgtagttgg cgatggtaac
    10381 ggtcgcgttg gttttggtta cggtaaagcg cgtgaagttc cagcagcgat ccagaaagcg
    10441 atggaaaaag cccgtcgcaa tatgattaac gtcgcgctga ataacggcac tctgcaacac
    10501 cctgttaaag gtgttcacac gggttctcgc gtattcatgc agccggcttc cgaaggtacc
    10561 ggtatcatcg ccggtggtgc aatgcgcgcc gttctggaag tcgctggggt tcataacgtt
    10621 ctggctaaag cgtatggttc caccaacccg attaacgtgg ttcgtgcaac tatcgatggt
    10681 ctggaaaata tgaattctcc agaaatggtc gctgccaagc gtggtaaatc cgttgaagaa
    10741 attctgggga aataaaccat ggcaaagact attaaaatta ctcaaacccg cagtgcaatc
    10801 ggtcgtctgc cgaaacacaa ggcaacgctg cttggcctgg gtctgcgtcg tattggtcac
    10861 accgtagagc gcgaggatac tcctgctatt cgcggtatga tcaacgcggt ttccttcatg
    10921 gttaaagttg aggagtaaga gatgcgttta aatactctgt ctccggccga aggctccaaa
    10981 aaggcgggta aacgcctggg tcgtggtatc ggttctggcc tcggtaaaac cggtggtcgt
    11041 ggtcacaaag gtcagaaatc tcgttctggc ggtggcgtac gtcgcggttt cgagggtggt
    11101 cagatgcctc tgtaccgtcg tctgccgaaa ttcggcttca cttctcgtaa agcagcgatt
    11161 acagccgaag ttcgtctgtc tgacctggct aaagtagaag gcggtgttgt agacctgaac
    11221 acgctgaaag cggctaacat tatcggtatc cagatcgagt tcgcgaaagt gatcctggct
    11281 ggcgaagtaa cgactccggt aactgttcgt ggcctgcgtg ttactaaagg cgctcgtgct
    11341 gctatcgaag ctgctggcgg taaaatcgag gaataagtag cagatggcta aacaaccggg
    11401 attagatttt caaagtgcca aaggtggctt aggcgagctg aaacgcagac tgctgtttgt
    11461 tatcggtgcg ctgattgtgt tccgtattgg ctcttttatt ccgatccctg gtattgatgc
    11521 cgctgtactt gccaaactgc ttgagcaaca gcgaggcacc atcattgaga tgtttaacat
    11581 gttctctggt ggtgctctca gccgtgcttc tatctttgct ctggggatca tgccgtatat
    11641 ttcggcgtcg atcattatcc agctactgac ggtggttcat ccaacgttgg cagaaattaa
    11701 gaaagaaggg gagtctggtc gtcgtaagat cagccagtac acccgctacg gtactctggt
    11761 gctggcaata ttccagtcga tcggtattgc tactggtctg ccgaatatgc ctggtatgca
    11821 aggcctggtg attaacccgg gctttgcatt ctacttcacc gctgttgtaa gtctggtcac
    11881 aggaaccatg ttcctgatgt ggttgggcga acagatcact gaacgaggta tcggcaacgg
    11941 tatttcaatc attatcttcg ccggtattgt cgcgggactc ccgccagcca ttgcccatac
    12001 tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg ttgctgttgg ttgcagtatt
    12061 agtatttgca gtgacgttct ttgttgtatt tgttgagcgt ggtcaacgcc gcattgtggt
    12121 aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct gcacagagca cacatttacc
    12181 gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc gcttccagta ttattctgtt
    12241 cccggcgacc atcgcgtcat ggttcggggg cggtactggt tggaactggc tgacaacaat
    12301 ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta ctctatgcgt ctgcaatcat
    12361 cttcttctgt ttcttctaca cggcgttggt tttcaacccg cgtgaaacag cagataacct
    12421 gaagaagtcc ggtgcatttg taccaggaat tcgtccggga gagcaaacgg cgaagtatat
    12481 cgataaagta atgacccgcc tgaccttggt tggtgcgctg tacattacct ttatctgcct
    12541 gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc tacttcggtg ggacctcact
    12601 gcttatcgtt gttgtcgtga ttatggactt tatggctcaa gtgcaaactc tgatgatgtc
    12661 cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa ggctacggcc gataattggt
    12721 cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc ttccgtcaag aaattatgcc
    12781 gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt gatttgcagt gccgagccga
    12841 agcataaaca gcgccaaggc tgattttttc gcatattttt cttgcaaagt tgggttgagc
    12901 tggctagatt agccagccaa tcttttgtat gtctgtgcgt ttccatttga gtatcctgaa
    12961 aacgggcttt tcagcatgga acgtacatat taaatagtag gagtgcatag tggcccgtat
    13021 agcaggcatt aacattcctg atcataagca tgccgtaatc gcattaactt cgatttatgg
    13081 cgtcggcaag acccgttcta aagccatcct ggctgcagcg ggtatcgctg aagatgttaa
    13141 gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt gacgaagttg ccaaatttgt
    13201 cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc aagcgcctga tggatcttgg
    13261 ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt cgcggtcagc gtaccaagac
    13321 caacgcacgt acccgtaagg gtccgcgcaa accgatcaag aaataatcgg ggtgattgaa
    13381 taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag aaaacaagtc tctgacggcg
    13441 tggctcatat ccatgcttct ttcaacaaca ccatcgtgac tatcactgat cgtcagggta
    13501 acgcgttggg ttgggcaaca gccggtggtt ccggtttccg tggttctcgc aaatccactc
    13561 cgtttgcagc acaggttgca gcagagcgtt gcgctgacgc cgtgaaagaa tacggcatca
    13621 agaatctgga agttatggtt aaaggtccgg gtccaggccg cgaatctact attcgtgctc
    13681 tgaacgccgc aggtttccgc atcactaaca ttactgatgt gactccgatc cctcataacg
    13741 gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcctcgt tttccaggtt tgttggagaa
    13801 agaaaatggc aagatatttg ggtcctaagc tcaagctgag ccgtcgtgag ggcaccgact
    13861 tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg taaaattgaa caagctcctg
    13921 gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg tgtgcagttg cgtgaaaagc
    13981 aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt ccgtaactac tacaaagaag
    14041 cagcacgtct gaaaggcaac accggtgaaa acctgttggc tctgctggaa ggtcgtctgg
    14101 acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc agaagcacgt cagctggtta
    14161 gccataaagc aattatggta aacggtcgtg ttgttaacat cgcttcttat caggttagtc
    14221 cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca gtctcgcgtg aaagccgctc
    14281 tggagctggc tgagcagcgt gaaaagccaa cctggctgga agttgatgct ggcaagatgg
    14341 aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc tgcggacatt aacgaacacc
    14401 tgatcgtcga gctttactcc aagtaaagct tagtaccaaa gagaggacac aatgcagggt
    14461 tctgtgacag agtttctaaa accgcgcctg gttgatatcg agcaagtgag ttcgacgcac
    14521 gccaaggtga cccttgagcc tttagagcgt ggctttggcc atactctggg taacgcactg
    14581 cgccgtattc tgctctcatc gatgccgggt tgcgcggtga ccgaggttga gattgatggt
    14641 gtactacatg agtacagcac caaagaaggc gttcaggaag atatcctgga aatcctgctc
    14701 aacctgaaag ggctggcggt gagagttcag ggcaaagatg aagttattct taccttgaat
    14761 aaatctggca ttggccctgt gactgcagcc gatatcaccc acgacggtga tgtcgaaatc
    14821 gtcaagccgc agcacgtgat ctgccacctg accgatgaga acgcgtctat tagcatgcgt
    14881 atcaaagttc agcgcggtcg tggttatgtg ccggcttcta cccgaattca ttcggaagaa
    14941 gatgagcgcc caatcggccg tctgctggtc gacgcatgct acagccctgt ggagcgtatt
    15001 gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg acctggacaa gctggtcatc
    15061 gaaatggaaa ccaacggcac aatcgatcct gaagaggcga ttcgtcgtgc ggcaaccatt
    15121 ctggctgaac aactggaagc tttcgttgac ttacgtgatg tacgtcagcc tgaagtgaaa
    15181 gaagagaaac cagagttcga tccgatcctg ctgcgccctg ttgacgatct ggaattgact
    15241 gtccgctctg ctaactgcct taaagcagaa gctatccact atatcggtga tctggtacag
    15301 cgtaccgagg ttgagctcct taaaacgcct aaccttggta aaaaatctct tactgagatt
    15361 aaagacgtgc tggcttcccg tggactgtct ctgggcatgc gcctggaaaa ctggccaccg
    15421 gcaagcatcg ctgacgagta accggatcac aggttaaggt tttactgaga aggataaggt
    15481 catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac agcagccatc gccaggctat
    15541 gttccgcaat atggcaggtt cactggttcg tcatgaaatc atcaagacga ctctgcctaa
    15601 agcgaaagag ctgcgccgcg tagttgagcc gctgattact cttgccaaga ctgatagcgt
    15661 tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac gagatcgtgg caaaactgtt
    15721 taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt tacactcgta ttctgaagtg
    15781 tggcttccgt gcaggcgaca atgcgccgat ggcttacatc gagctggttg atcgttcaga
    15841 gaaagcagaa gctgctgcag agtaatctga agcaacgtaa aaaacccgcc ccggcgggtt
    15901 tttttatacc cgtagcatcc ccacttatct acaatagctg tactcttttt gttcatcccc
    15961 tggagtattt atgtggttac ttgaccagtg ggcagagcgt catatagcag aagcgcaagc
    16021 gaaaggtgag tttgataacc tggtaggtag cggcgaacca ttgatactgg atgatgattc
    16081 tcatgtgcca ccggaattac gtgcggggta tcgcttgctg aagaatgctg gttgcttacc
    16141 gccagaactt gagcatagga gggaagcaat acagcttctg gatattctca aaggtatccg
    16201 tcatgatgat ccgcaatatc aagaggttag tcgtcgattg tcattactgg aattgaagct
    16261 gcgacaagct ggattgagta ccgatttttt acgcggcgat tatgctgaca agttgttgaa
    16321 caaaatcaac gataactagt ggagtatgta tgtatcgcat tggtgagctg gcaaaaatgg
    16381 cggaagtaac acccgacacg attcgttatt acgaaaagca gcagatgatg gagcatgaag
    16441 tacgcaccga aggtgggttt cgcctgtata ccgaaagcga tctccagcga ttgaaattta
    16501 tccgccatgc cagacaacta ggtttcagtc tggagtcgat ccgcgagttg ctgtcgatcc
    16561 gtatcgatcc tgaacaccat acctgtcagg agtcaaaagg cattgtgcag gaaagattgc
    16621 aggaagtcga agctcgtata gccgagttgc agagtatgca gcgttccttg caacgcctta
    16681 atgatacctg ttgtgggacc gcccatagca gtgtttattg ctcgattctt gaagctcttg
    16741 aacaaggggc gagtggcgtt aagagtggct gttgattttt tgcactggca ggattacact
    16801 cgcgccgtta aataaccaac tggagttttt atgagccgat atcagcatac taaagggcag
    16861 ataaaggata atgcgataga agcattacta catgatccct tattccgaca gcgagtagag
    16921 aaaaataaga aagggaaagg cagttacatg cgaaaaggaa aacatggcaa tcgggggaac
    16981 tgggaggcca gtggcaagaa agtaaatcac ttttttacca ctggccttct gctttcagac
    17041 gcttgttaag agcggttatt ctgctctttc agcaaatcac gaatttctgt cagtaatact
    17101 tcttctttag ttggtgcagg tgttgctgct gctggttctt cttttttccg attcagtttg
    17161 ttgattagct taatcgccat aaagatggca aaggccacta tcagaaaatc aaagacgttt
    17221 tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccttgggc ctcgcgtagc
    17281 gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg
    17341 atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg ctccgataat gacacccact
    17401 gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg
    17461 ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca ttttcctgcc
    17521 aggaatggat ttaattaatc cttaataatc aaagggaaat aataaggcgt cattagacgc
    17581 cttattaatt acaagaagaa aggacttggc tggaagagtc tttcgacgtc ggtaataaac
    17641 tttttatctg tgaggaacat aattacgtga tcaccttgct caatgcgcag attgtcattg
    17701 gcaatcatca catcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg
    17761 atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct
    17821 tcaatagctt ctgctacgcc gcggcgtaat gaggaaacac caacaatatc tgcttttcgc
    17881 acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaatcgcaat atcgatcacg
    17941 ctcccctgaa ccagatccac ataagcgcga cgttggatca ataccatcac ctttttcgca
    18001 cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca
    18061 ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca
    18121 ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta
    18181 cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata
    18241 ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc
    18301 atcaccgcac ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttcaaca
    18361 atggtcgaac cttgcggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta
    18421 tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcat taccaatcag cgggccgcca
    18481 taataggctt taaccacagc caggctgact ttaccctcag cgaagttcac cacctgcaat
    18541 gcgccgggat actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc
    18601 agatgatcaa tcggtacagc atctgaatga aatagtttat cggcatcgcg cacgtagtct
    18661 ggtgagcgga tacgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag
    18721 gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca
    18781 cctgcctccc gcaatacgcg tggatgagag ccatgcccct gtacgacccg caggtcaaat
    18841 ttatcctgta aggtccgcag acgctcaccg ttggtatcga caacagtaat atcgttgttc
    18901 tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt
    18961 ttcatcagtc gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg
    19021 tcgccctctt cggcaccggg caaattttgc ttacctggtt gttctggtgt tccggtttcg
    19081 caaagcattg catcttctgt acgttgcaga aaggctttaa tttgttggct attctcttcc
    19141 ggtaacactg aacaggtggc atagaccagg gttccaccgg attttaaatg cggccagatg
    19201 gcgtcgagaa tttcagactg caactgcgcg agttcaggga tatcgcgatc gcgacgtaac
    19261 catttaatat ccggatggcg acgaatcacc cccgttgctg aacaaggcgc atctaataaa
    19321 atgcgatcaa actgttgctc gccacaccat tgggaagggt agcggccatc accttgtttc
    19381 acggtcgcct tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgctca
    19441 tcaatatcga ccgccagaac ttgcgcctct ggagcaacct caagaatatg cgttgtttta
    19501 ccgcctggcg cggcgcaaag atccagaatg tgttcaccat tttgtggagc aagccaggtc
    19561 atgcaacctt gtgctgaagc atcctgaacg gtaacccatc cttcttcaaa accgggtagc
    19621 gcatgaacag gtgcaggtgt ttccagctgt acagcatcag ggtaatccgc atgcgggaaa
    19681 cctttcattc ctgctttatc cagcaatgca agccagctgt cgcgggaatg atgcgtacga
    19741 ttgacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc
    19801 tcgggatacg ccttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca
    19861 ctggcattaa actcggctaa taactcatct tgctgacgct ggaactgacg taatacaccg
    19921 ttaatcaatc ccttaagttg cgggcgctta attgcaactg cgccttcaac cgtttcagcc
    19981 agcgcggcat gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg
    20041 taatgaacag tacgctgttt gcctgtcatc ggacgggcca ttagcttatt aatcagccag
    20101 tctgactgtg agagcgtacg cagtacgcca aagcataact cctggagaag agctttatct
    20161 ttatcggaaa ctttttgctg gagcggtggc agaatgttgc tcagtgattg cccttgctca
    20221 acgacttgtt caatggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt
    20281 ataaatataa agatgcccgg taagaccggg cttagaagag tggactatca ggccagacgg
    20341 ttgcccggta caaaccattc ccgacgagaa ttcaggaggt cttgcacgct catcgctttc
    20401 ttacctgcag gttgcattga gagcagattc aggatgccat cacctgtcgc tacctgaatg
    20461 ccttgtttat tggcttcaag gattgttcct ggcgcagcct ttgttgtcgt atcgatgaca
    20521 gatgctttcc agactttaac cggctgccct tcaatttcca gccagctcat tggccatgga
    20581 ttgaaagcgc gaatgcaacg ttcaagctgt gctgccgaaa gtgaccagtc aatacgcgct
    20641 tcttctttac tcaacttttc ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc
    20701 gccgtgccgt ctgccagttg tttcaacgtg gtgataagcc cttgtgggcc aagctctgcc
    20761 agcttgtcgt acagcgtacc actggtatct tctgcagtaa tcgggcaggt gagcttatag
    20821 agcatgtcac cggtgtctaa accaacatcc atttgcataa tggtcacacc agtttctgca
    20881 tcacccgccc atagtgagcg ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa
    20941 ccatgaacgt tgatacagcc aagacgcggc atctccagca cagctttagg cagaattaaa
    21001 ccataggcga cgacgaccat aacatcagcc tgcagatcgg cgaccagttg ctggttttct
    21061 tgtggacgca gggaaacagg ttgaaaaacg ggcagacctt tgtcctcagc cagaacttta
    21121 accgggctgg gcatcagttt tttaccgcgt cctgccggtc ggtctggctg ggtgaacacg
    21181 ccaacgatgt tatgaccaga agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt
    21241 gtacccgcaa aaataatacg tagtgattct gacacgttaa ttcttatcct taagcccggg
    21301 ctttcagacg atccagtttt tcaactttct gacgaatacg ttgttgtttc agcggtgata
    21361 gataatccat aaacagtttg ccgaccaggt gatccatctc atgctgaata cagatggcta
    21421 acagaccgtc tgcttccagt tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa
    21481 ctttctctgc gcgtggcact aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa
    21541 tgcctgtttc gccgcttttt tctaaaagct ccggattgat taacaccagc cgttcgtcac
    21601 ggttttccga aacatcaatt acaatgatac gttgatggat atcaacctgg gttgccgcca
    21661 ggccaatacc ttcttctgcg tacatcgtct cgaacatatc atcgacgata cgctgaattt
    21721 ctgcattcac ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta
    21781 acacttgcaa aactgacata aatctccaga gatgtgttca ggagttagaa agattatttc
    21841 ttctattcta gacaaatccc cctctgattg acagcatcac tgaccaatcg caaagattgc
    21901 taaggctgct tatggcaggg agataaggat gttcgataca gaaatttggc tgcgtttaat
    21961 gagcatcagc agcttgtatg gcgatgatat ggtccgtata gctcactggc tggcaaaaca
    22021 gtcgcatatt gatgcggttg tattgcagca aacagggctt acattgcggc aggcacaacg
    22081 ctttctttca tttccgcgaa agagtatcga aagctcactt tgttggttgg agcaacccaa
    22141 ccatcattta atccctgcgg acagcgaatt ttatcctcct caacttctgg cgacgacaga
    22201 ttaccccggc gcactgtttg ttgaaggaga actgcacgcg ttgcattcat ttcagcttgc
    22261 cgtagtgggg agtcgggcgc attcatggta tggcgagcga tggggacgat tattttgcga
    22321 aactcttgcg acgcgtggag tgacaattac gagtggactg gcgcgtggaa tcgatggtgt
    22381 ggcgcataaa gcgaccttac aggtaaatgg cgttagcatt gctgtattgg ggaatggact
    22441 taataccatt catccccgcc gccatgctcc actggctgcc agtctacttg agcaaggtgg
    22501 tgctctcgtc tcggaatttc ccctcgatgt tccacccctt gcttacaatt tcccacgaag
    22561 aaatcgcatt atcagtggtc taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag
    22621 tggttcgctg gtgacagcac gttgtgcgct tgagcagggg cgtgaagttt ttgctttgcc
    22681 aggtccaata gggaatccgg gaagcgaagg gcctcactgg ttaataaaac agggggcgat
    22741 tcttgtgacg gaaccggaag aaattctgga aaacttgcaa tttggattgc actggttgcc
    22801 agacgcccct gaaaattcat tttattcacc agatcaggaa gacgtggcat tgccatttcc
    22861 tgagctcctg gctaacgtag gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc
    22921 cggccaacct gtgccagagg tagttactca actactcgaa ctggagttag caggatggat
    22981 cgcagctgta cccggcggct atgtccgatt gaggagggca tgccatgttc gacgtactaa
    23041 tgtatttgtt tgaaacctat attcacacag aagctgagtt gcgtgtggat caagacaaac
    23101 ttgaacagga tcttaccgac gcaggatttg atcgagaaga tatctacaat gccctgcttt
    23161 ggctggaaaa gcttgcggat tatcaggaag gactggcaga accgatgcaa ctggcctctg
    23221 atccactctc catgcgtatt tatacccctg aagagtgtga aagactggat gccagctgtc
    23281 gtggttttct gcttttcctt gagcaaattc aggtgctcaa ccttgaaacc cgtgaaatgg
    23341 tgatagagcg agtgctcgcg ctggataccg ctgagttcga cctggaagac ctgaaatggg
    23401 taatcctgat ggtgttgttc aatattccgg gctgcgaaaa tgcgtaccag caaatggaag
    23461 aattactctt tgaagtgaat gaaggtatgc tgcattaatc atttctttaa ttcagcataa
    23521 gtagttatgg cgaaatcagc actgttcacg gtgcgtaata atgagtcctg cccaaagtgc
    23581 ggggctgaac tagttattcg atccgggaaa cacggtccgt ttcttggatg ctcacagtat
    23641 ccggcgtgtg actacgtccg tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt
    23701 ctggaggggc aggtttgccc tgcatgtggc gcaaatctgg tattacgcca aggacgcttt
    23761 ggtatgttta ttggttgcag taactaccct gaatgcgaac ataccgaact tatcgataaa
    23821 ccggacgaaa cagcaattac atgcccccaa tgccggacgg gccatctggt tcagcgccgc
    23881 tcccgttatg gcaaaacatt tcactcctgt gatcgctacc cggagtgtca atttgccatt
    23941 aacttcaaac ccatagctgg agaatgccct gagtgccatt atccgctact catcgaaaag
    24001 aaaaccgcgc agggtgtaaa acacttttgt gccagtaaac aatgtggaaa gccgatttcg
    24061 gcggaataat aacgtgaata ataacctgca aggagacgct atcgcagctg cgatagatgt
    24121 cctcaatgaa gaacgtgtca tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga
    24181 tcctgatagc gaaacagcag tgatgagact gttggagtta aaacggcgtc cggttgataa
    24241 ggggctgatt ttaatcgcgg caaattacga gcagcttaaa ccctatattg atgacaccat
    24301 gctgactgac gcgcagcgtg aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt
    24361 ctttcccgcg cctgcgacaa caccgcgctg gttgacgggc cgctttgatt cgcttgctgt
    24421 acgagtcacc gaccatccgt tggtggttgc tttgtgccag gcttatggta aaccgctggt
    24481 ttctaccagt gccaacttga gtggattgcc accttgtcga acagtagacg aagttcgcgc
    24541 acaatttggc gctgcgttcc cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc
    24601 agaaatccgc gatgccctga cgggtgaact gtttcgacag gggtaacata atggaaacct
    24661 atgctgtttt tggtaatccg atagcccaca gcaaatcgcc attcattcat cagcaatttg
    24721 ctcagcaact gaatattgaa catccctatg ggcgtgtgtt ggcacccatc aatgatttca
    24781 tcaacacact gaacactttc tttagtgctg gtggtaaagg tgcgaatgtg acggtacctt
    24841 ttaaagaaga ggcttttgcc agagcggatg agcttactga acgggcagcg ttggctggtg
    24901 ctgttaatac cctcaagcgg ttagaagatg gatgcctgct gggtgacaat accgatggta
    24961 taggtttgtt aagcgatctg gaacgtctgt cttttatccg tcctggttta cgtattctgc
    25021 tcattggcgc tggtggagca tctcgcggcg tactactgcc actcctttcc ctggactgtg
    25081 cggtgacaat cactaatcgg acggtatctc gcgcagaaga gttgactaaa ttgtttgcgc
    25141 acactggcag tattcaggcg ttgggtatgg acgaactgga aggtcatgag tttgatctca
    25201 ttattaatgc aacatccagt ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca
    25261 ttcatccagg catttattgc tatgacatgt tctatcagaa aggaaaaact ccttttctgg
    25321 catggtgtga gcagcgaggc tcaaagcgta atgctgatgg tttaggaatg ctggtggcac
    25381 aggcggctca tgccttcctt ctttggcatg gcgttctgcc tgatgtagaa ccggtcatca
    25441 aactattgca acaggaattg tcagcgtgaa tcaggccatc cagtttccgg atagggaaga
    25501 gtgggacgag aataaaagat gtgtatgttt tcccgctctc gtgaatggta tgcaactgac
    25561 atgtgcgatc tctggcgaga gtctggcgta tcgctttact ggagatacgc cagaacagtg
    25621 gttagcgagt tttcgtcagc atcgctggga cctggaagaa gaagcggaaa acttaattca
    25681 ggaacaaagt gaagatgatc aaggttgggt ctggttacct tgatccagat attcgtcctt
    25741 ccatttcacg tagttatttg cggaatagcg taacccagcc ttctcttcat cacttaacgg
    25801 gcggatctgt ttgacggggc taccgagata cagatatccg ctctccagcc gtttattttg
    25861 tgggaccagg ctacccgcac caatcatcac atcatcttct actattgcgc catcaagtaa
    25921 aattgagccc atcccaacca aaactcgatt gccaatggtg cagccgtgga gcatcacctt
    25981 gtgaccaaca gtgacatctt cgccaatggt taatgggttg ccatctgggt tgtacgagga
    26041 tttatgagtg acatgcaaca tactgccatc ctggatattg gtgcgtgctc cgatctgtac
    26101 ataatgtaca tctccacgaa tcacaacgag cggccagatc cccacatcat cagccagacg
    26161 aacgtcacca atcacgacgc tgctatcgtc gatcattacg cgctgaccga tttgtggaaa
    26221 aagatcgcgg tatgggcgta aaacatcaga catactcacc tcaacaataa atgatttact
    26281 aatgactttg ggtgcattat tggccttgtg caagtctttt agcatgcaaa aaagcaccgt
    26341 tttgtgtgcg aatgcagcaa aaagggtgaa aaagcaacaa acagaaaaaa agatcaaaaa
    26401 agtacttgtg caaaaaattg ggatccctat aatgcgcctc cgttgagacg acaacgtgaa
    26461 acacttcaca agatggtcgt aacaacaaag agaaaaaatc ctgaa
//
LOCUS       DADSZD010000054        26338 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.054, whole genome shotgun sequence.
ACCESSION   DADSZD010000054 DADSZD010000000
VERSION     DADSZD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.054"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     repeat_region   335..1022
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=335..362
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            complement(1135..1353)
                     /gene="infA"
                     /locus_tag="J1Z28_001992"
     CDS             complement(1135..1353)
                     /gene="infA"
                     /locus_tag="J1Z28_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBA7716829.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(1638..2342)
                     /gene="aat"
                     /locus_tag="J1Z28_001993"
     CDS             complement(1638..2342)
                     /gene="aat"
                     /locus_tag="J1Z28_001993"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBA7716830.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDGLVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(2384..4105)
                     /gene="cydC"
                     /locus_tag="J1Z28_001994"
     CDS             complement(2384..4105)
                     /gene="cydC"
                     /locus_tag="J1Z28_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBA7716831.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(4106..5872)
                     /gene="cydD"
                     /locus_tag="J1Z28_001995"
     CDS             complement(4106..5872)
                     /gene="cydD"
                     /locus_tag="J1Z28_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBA7716832.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLPLLPQGIDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(5995..6960)
                     /gene="trxB"
                     /locus_tag="J1Z28_001996"
     CDS             complement(5995..6960)
                     /gene="trxB"
                     /locus_tag="J1Z28_001996"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBA7716833.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            7504..7998
                     /gene="lrp"
                     /locus_tag="J1Z28_001997"
     CDS             7504..7998
                     /gene="lrp"
                     /locus_tag="J1Z28_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBA7716834.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            8133..12215
                     /gene="ftsK"
                     /locus_tag="J1Z28_001998"
     CDS             8133..12215
                     /gene="ftsK"
                     /locus_tag="J1Z28_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBA7716835.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQYQQ
                     PQQPVAQQPQYQQPQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHDTLLHPLLM
                     RNGDSRPLNKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVN
                     YSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNK
                     KRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSV
                     GVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCV
                     NEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPY
                     IVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIP
                     TRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVV
                     QDWKARGRPQYVDGITSDSESEGGVGGFDGAEELDPLFDQAVQFVTEKRKASISGVQR
                     QFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            12374..12985
                     /gene="lolA"
                     /locus_tag="J1Z28_001999"
     CDS             12374..12985
                     /gene="lolA"
                     /locus_tag="J1Z28_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBA7716836.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            12996..14339
                     /locus_tag="J1Z28_002000"
     CDS             12996..14339
                     /locus_tag="J1Z28_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBA7716837.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            14430..15722
                     /gene="serS"
                     /locus_tag="J1Z28_002001"
     CDS             14430..15722
                     /gene="serS"
                     /locus_tag="J1Z28_002001"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBA7716838.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            15961..18405
                     /gene="dmsA"
                     /locus_tag="J1Z28_002002"
     CDS             15961..18405
                     /gene="dmsA"
                     /locus_tag="J1Z28_002002"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBA7716839.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGIPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGISNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            18416..19033
                     /gene="dmsB"
                     /locus_tag="J1Z28_002003"
     CDS             18416..19033
                     /gene="dmsB"
                     /locus_tag="J1Z28_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBA7716840.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNATKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            19035..19898
                     /locus_tag="J1Z28_002004"
     CDS             19035..19898
                     /locus_tag="J1Z28_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBA7716841.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTLMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(19934..20560)
                     /locus_tag="J1Z28_002005"
     CDS             complement(19934..20560)
                     /locus_tag="J1Z28_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA7716842.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            20874..22022
                     /locus_tag="J1Z28_002006"
     CDS             20874..22022
                     /locus_tag="J1Z28_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7716843.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGIGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTHVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(22119..22859)
                     /gene="pflA"
                     /locus_tag="J1Z28_002007"
     CDS             complement(22119..22859)
                     /gene="pflA"
                     /locus_tag="J1Z28_002007"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBA7716844.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(23050..25332)
                     /gene="pflB"
                     /locus_tag="J1Z28_002008"
     CDS             complement(23050..25332)
                     /gene="pflB"
                     /locus_tag="J1Z28_002008"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBA7716845.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(25387..26244)
                     /gene="focA"
                     /locus_tag="J1Z28_002009"
     CDS             complement(25387..26244)
                     /gene="focA"
                     /locus_tag="J1Z28_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBA7716846.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         6558 a         6549 c         6903 g         6328 t
ORIGIN      
        1 ttgctgccag acctcatagg tggcaacggg gcgctactat agggagttgg attgaaacgg
       61 tcaagaagaa tttatataga ttgatttgtt tggttacgca atgaacacgc tgttcgcggg
      121 acggagatta tgaccgtatg tgttctggtc aattgtttat caaaagctat gcagaaaata
      181 tgagattgaa gaaataccaa accgaccctt tttctaggtt gtaatgtaac tcattgattt
      241 ttttattgct attttgaagt ctggaaaaag ggtttgaatc tgcgattttg taagttttaa
      301 cagtaaatca atcggatagt ctgctattat tccagttcac tgccgtacag gcagcttaga
      361 aagcacattc ccggcctctt gtttccctgg tggcgttcac tgccgtacag gcagcttaga
      421 aagaaaacac cggcgagatc gtattttttg ccgcgttcac tgccgtacag gcagcttaga
      481 aatcgtctat attttcaaat tcttctcgaa ccgcgttcac tgccgtacag gcagcttaga
      541 aaagcaaacc acgctcggta gtgaactacg catcgttcac tgccgtacag gcagcttaga
      601 aaaggaaggt ggtaagaagc agattgcgct gaccgttcac tgccgtacag gcagcttaga
      661 aagttcaatt atcaaatatc acccgcgata tggtgttcac tgccgtacag gcagcttaga
      721 aaacatttaa actaacaatt ggtgagcctg atatgttcac tgccgtacag gcagcttaga
      781 aatgagcacg tctgccgccg ggcgaccaac tggcgttcac tgccgtacag gcagcttaga
      841 aaccagctgg atgaacaaag gcgaaccgca aaagcgttca ctgccgtaca ggcagcttag
      901 aaaacgaatg cgcttacatc ctggcaaaag ggcgtgttca ctgccgtaca ggcagcttag
      961 aaaaaaacat ggcgctggac cagacccgca aggtgttcac tgccgtacag gcagtcttca
     1021 aactcaacac tccatcctct aatatttatt ccccataact cacagacgca aaaaaggccg
     1081 gctaaaccga ccttttactc attctttctc ttcgcccatc aggcggtaaa acaatcagcg
     1141 actacggaag acaatgcggc ctttgctcag gtcgtacggg gtcagttcaa cagtcacttt
     1201 gtcgcccgtc aggatgcgga tgtagttttt gcgcatttta ccggagatgt gtgcagtaac
     1261 cacgtgaccg ttttctaact ctacgcggaa catggtatta ggcaacgttt caagaacggt
     1321 accttgcatt tcaatattgt cttctttggc catctaatcc tctggggtat cactaccgta
     1381 atttgaaccg gcaagataat gccgaagttc tgtaaataag taaagatttg cgcgctaaat
     1441 cgcaacaaac aggttcggca cattactccg aaaacacacg gctaagccgc accaaaagcg
     1501 caacgtataa gggagcggtg agataaacga tgggcgttac ctgacgcgaa aaattcctta
     1561 tcggcagcgg ggtaatgagc gtaaccaact ctgcgaccgc aattataaca ctctggggag
     1621 aaatgtgccg aaaacattca ttcttgtggt gaaaacaagc atcgaggtac ccagaaatta
     1681 ttcggcaatc gtccgaggcg catttgattg agataattaa ggtaatcccg gcggggaatt
     1741 tcgcaggcac caagcgatgc tgtgtgatcg ttaaggacct ggcagtcgat aagtttaccg
     1801 ccatgacgga taaattcatc acagaagacc agaagcgccg ttttggacgc attttccatc
     1861 cggctgaaca tggactcgcc acaaaatagt gttccctggg ccacgccgta cataccgccg
     1921 acaagcccat cttcacgcca gacttcaatg gagtgggcat gaccgagttc gtgaaggcga
     1981 tggtaggctt cgaccacgcc acgcgtgatc caggttcctt cttcgcgatc gctggcacag
     2041 ccttcgatga cctgaccaaa agcgtaattc atcgtgacac gatagggtga gcgtttatga
     2101 aatcgcttca tactacggct gatatgcagt gattctggcc ataacaccgc gcggggatcg
     2161 ggcgaccacc agaggatggg gtcgccagga gaaaaccacg ggaaaatacc acgctgataa
     2221 gccattaata ggcgcgcagg gctaagatcg ccccccagtg ccagcaggcc gttaggctca
     2281 cgtaatgcgc cttccgggga agggaaggct attgaatggc gagaaagctg aaccaggcgc
     2341 atgaccgcaa aactccacgc aagtcggatc gttcaataat agcttacaaa ccctgcttaa
     2401 actggtaata acgcccctgt ctggcaagca gttctgcgtg agtaccttgc tcaataattt
     2461 gcccgttgtc catcactatt atttgttgga aacgagagag tccgcgaagt cgatgggtga
     2521 ccattaacac cgttttctcg cacatcattt ctgcaagcaa ttcaaggatc tgactttcgg
     2581 ttgtggcatc taagccttct gtaggttcat ccagcaatac cagcggcgca tcatgtaaca
     2641 gcgcacgggc gatagccaga cggcgcagtt caccaccgga gagctggcgt ccgccttcac
     2701 ctaaccaact gttgagacct gcatcctcga gcagcttctc cagaccaacg cgacgcaaga
     2761 tctccgccag agcctcatca ctactaccag gcgaggcgag taacagatta tcacgcagcg
     2821 tggcgctaaa cagatgcact cgctggggaa caacgctgat ggtctgtcgt agagccgctt
     2881 cattcaggct ggctatggga ctatcgttaa gcaaaatctc gccctgttgc gggtcccatg
     2941 cgcgggtcag cagttgtaac agtgttgatt tgccgcatcc ggttcgcccg agaatcgcaa
     3001 tatgttcccc ggcgtttacc tgaagataaa tccctttaag tgcctgttgg gattgctccg
     3061 gataagtgaa ctgaacatcc cgtaatgtca gcgaaacgcg atcggcaaca cgagtttggg
     3121 tatcagggaa ggtgacttcc ggtttttgat ccgttaaatc agtgatccgt acggcagagg
     3181 caatgacttg ccccagatgc tgaaatgcac ccgttactgg tgccagcgct tcaaacgcgg
     3241 ctaacgcgca gaagacaaac agggcaatta acgcgccggg ttgagcattg ccgccaacgc
     3301 cgccagacgc catccacagc atcaggatca ccgctaacgc gccaatgagc agcattatcg
     3361 cttgcgacaa tgcggtcagt tcagattgac ggcgttgcgc ttccagccac tgaatttctg
     3421 tattctctaa ttgcgtgcga taacgatcgc tggcaccaaa aatggtcaac tcagcttgtc
     3481 cttgcagcca ggccgtcagt tgttggcgat actgcccgcg aagatgagtc agattttgcc
     3541 cggtgctttt tcccgcacga taaaacagtg gtggcatcag gaaaagcgtc agtaacataa
     3601 tgccgcccag cgtaaaggcg agggtgaaat caaggaaact taacccgatt gtcaccacca
     3661 taatcaccac aaaagcgcct accagcggtg agataacgcg caggtaaaga tgatcgagcg
     3721 tatcaacatc cgccaccacg cggttgagta attcgccctg acgatagcgc gccagtccgg
     3781 caggggagag gggcagtaat ttgctgaagg tgtaaatgcg cagatgctgc aacacgcgga
     3841 aagtcgcatc gtgacttacc aggcgttcaa aatagcgccc ggcagtacgc gtgattgctg
     3901 cgccacgcac gcccgcagcg ggtagcatat agttgaagct gtacagtccg gcaacccccg
     3961 caaccgctga ggcggagagg aaccagcccg aaagtgtcaa cagaccgata ctggcgagca
     4021 gcgtgacaat tgccagcacg ataccaagac ttaacatcca tttatgacgt ttatacagtg
     4081 ccagataggg tagcaaagcg cgcatttaaa tctcctcctg acgatgggcc agtaatgtgg
     4141 cgaatgggcc gccagccaca cttaattccg cgtaacgtcc ttgctcaata atctgaccat
     4201 cctgcattac ccaaatgaca tcccagtcag caagatcttc taactggtgg gtgaccatta
     4261 acgttgtctg gcgcagagag gcggcattca gcgcctccat tacgcgctgt tcactgtgag
     4321 catcaaggct ggcagcgggt tcatccaaca gtaatagcga acagggattt agtaacgcac
     4381 gggccaccgc cacgcgctgc gcctgcccca cggaaaggcg ggcagcctgg tcaccaacag
     4441 gcgtatcaat gccttgcggc aggagcggta gaaactcact gacccaggcg ttatccagcg
     4501 ctgtttgtaa ctcttgttcg ctggcatcag gtcgtgccag tagtacgtta tcccgtaatg
     4561 ttgctgccgg taattgtggg ttttgcccaa cccaggagag atgtttacgc catgattccg
     4621 gtgataaatc gcgtaattct atcccgttga ttcgtagcga tccctgatat gagagaaaac
     4681 cagaaagcgc gttcaacagt gaacttttac ctgaaccgct gcgaccaacc aacactgctc
     4741 gttggcctgc tggcaaagta aagttcagcg gtccggccag cgttttacct tccggcgacg
     4801 tgataaacag atcctcggct tcaatggtca ccggatcggt cgatgctaat tccgcctcac
     4861 cgcgctgcgg atgggcgagc ggggtttcca taaacgtttt caaactgtca gctgcaccaa
     4921 cagcctgggc tttagcatga taaaacgtac cgagatcgcg taatggctgg aaaaactctg
     4981 gcgcaaggat cagggccaga aaacccgcag ccagcgtcac gccagtatcg tagtgaccaa
     5041 aatccagctc gccgaggtag gaaaaaccaa agtagaccgc caccagagca atcgacagcg
     5101 aggtaaaaaa ttcgagaatg ccggaggata aaaacgccag ccgtagcact tccattgtcc
     5161 gttggcggaa atcttccgaa gcagaacgaa tactttcaat ttcagcttca ccacgaccaa
     5221 aaatacgcaa tgtttccatg ccgcgcaggc gatcgaggaa atgcccactt aagcgagcaa
     5281 gagcgagaaa gttacgtcgg ttagcatcgg cagcccccat tccaaccagc gccataaata
     5341 acggaattag cggtgcagtg cccagcagaa tgagcgccgc agcccagtta gaggggaaga
     5401 tagccaccac aatcagcagc ggcaccgaca ctgccagcgc catttgcggc aggtagcgtg
     5461 catagtaatc atgcatatcg tcaatttgct cgagcaccag cgtcgcccag ctccccgcag
     5521 gtttaccctg aatccacgct ggccctgctt gttgcagacg gtcgagaacc tgacggcgga
     5581 tggcaaagcg gatatgctgc ccggcgtgat aacccacccg ttcgcgtaac cagaccaccc
     5641 atgcgcgcag tacaaaggtc agaaacagta acgtaaaggg aagcagcagg gcttcacggg
     5701 gaatattctc cataatcata tgttgcagaa ttcgcgccat gaaccaggcc tgggcaatga
     5761 tcaatatgcc gctcacaaag cccagcagac gagaaatatt cagccaacgt tgggagatga
     5821 cgctttgctg ttttaaccag cgggttaatt ctttttgacg ggatttattc attgcacgct
     5881 tagcaggtga gttatcagaa ttatttgcag agcaatgtta caacggggaa aaaataaagg
     5941 cgacccatag tcgcatggtg tcgccttctt tacttttgtt actgatttgt aaaattattt
     6001 tgcgtcagct aaaccgtcga ggtagcgttc cgcatcaaga gctgccatgc agcctgtacc
     6061 agcagatgta atggcctggc gataaatgtg atccatcacg tcacctgcgg caaagacgcc
     6121 agggatgctg gtctgggtgg cattaccatg aatacctgac tgtactttga tgtagccgtt
     6181 ttccagttcc agctgccctt cgaaaatggc agtattcggg ctgtgaccga tagcaacaaa
     6241 cagaccggca acgtcgagtg actcgatgtt atcgctgttt tgcgtatcgc gcagacgaac
     6301 gccagtgaca cccatctgat cgccggtcac ttcttccagc gtacggttgg tgtgcagaat
     6361 aatgttgccg ttctccactt tatccatcag acgcttaatg aggatttttt ccgcacggaa
     6421 accatcacgg cggtgaatca gatgaacttc cgaagcgatg ttagacagat acagcgcctc
     6481 ttcaaccgcg gtattgccgc cgccgatgac cgcaactttc tggttgcgat agaagaaacc
     6541 gtcgcaggtt gcacaagcag aaactccacg gcctttaaag gcttcttcag agggcaggcc
     6601 gagatagcgt gcagaagctc cggtggcaat aatcagcgcg tcgcaagtgt attcgccgct
     6661 atcgccagtc agacggaacg gacgattctg caaatcaact ttgttgatgt gatcaaagat
     6721 gatttcagtt tcaaatttgg tggcatgttc gtgcatgcgc tccattaata acggaccggt
     6781 cagatcgttt ggatcgccag gccagttttc cacttccgtg gtggtggtca gttggccgcc
     6841 tttttccatg ccggtaatca gcacaggttg caggttggcg cgcgccgcgt agacagcagc
     6901 ggtgtatccc gccgggcctg aacccaggat aagcagttta ctgtgtttgg tcgtgcccat
     6961 gagatcccca tagttgttgg cagacaatgg caggattgta gggaatttac agacgtaaaa
     7021 aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata agcaggtcaa
     7081 attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc cgattaaatg
     7141 atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc gatgttttgc
     7201 tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaac agtattctta
     7261 tatgcgcata accatgcatg taaataccat gtttaccgtg ctagtgaaat ctacgtatgg
     7321 cgtggacaga cgccattcgt gatgtcgata gctgccacaa ggcaacggtc ttctcaccgt
     7381 agacccaggc attgcgcgcc gtgaatcttc atgatttcgg tctatcgtga cgggtagcga
     7441 ctctgaacag tgatgtttca gggtcagaca ggagtaggga aggaatacag agagacaata
     7501 ataatggtag atagcaagaa gcgccctggc aaagatctcg accgtatcga tcgtaacatt
     7561 cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg agctttctaa acgtgtggga
     7621 ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg aaagacaagg gtttattcag
     7681 ggctatacgg cgctgctgaa cccccattat ctggatgcat cacttctggt attcgttgag
     7741 attactctga atcgtggcgc accggatgtg tttgaacaat tcaataccgc tgtacaaaaa
     7801 cttgaagaaa ttcaggagtg tcatttagta tccggtgatt tcgactacct gttgaaaaca
     7861 cgcgtgccgg atatgtctgc ttaccgtaag ttactggggg aaaccctgct gcgtctgcct
     7921 ggcgtcaatg acacacggac atacgttgtt atggaagaag tcaagcagag taatcgtctg
     7981 gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg cgtattttga ttacactcct
     8041 gttaatccat acagcaacag tactggggta acctggtact gttgtccgtt ttagcatcgg
     8101 gcaggaaaag cctgtaacct ggagagcctt tcttgagcca ggaatacact gaagacaaag
     8161 aagtcacatt gacaaagtta agtagcggtc gccgtcttct ggaggcgttg ctgatcctta
     8221 ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag ttttaaccct tcggacccca
     8281 gttggtcgca aacggcctgg catgaaccta tccataattt aggtgggatg cccggtgcgt
     8341 ggttggcaga tacgctgttc tttatttttg gcgtgatggc ttacaccatt cccgtcatta
     8401 ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag cgacgaatac attgattatt
     8461 ttgccgtttc gctacgcatc attggcgttt tggcgctcat ccttacctcc tgtggtctgg
     8521 cggcaatcaa cgctgacgat atctggtatt ttgcctccgg tggcgtcatt ggcagcttac
     8581 taagcactac gctacaacca ctgctacaca gtagcggggg aactattgcg ctgctctgcg
     8641 tttgggcagc gggcctgacg ctgttcaccg gttggtcatg ggtgaccatt gctgaaaaac
     8701 tcggcggctg gattttaaat attcttacct tcgccagtaa tcgtacccgt cgcgatgata
     8761 cctgggtcga tgaagatgag tatgaagacg acgaagagta tgaagatgaa aatcacggca
     8821 aacagcatga atcacgccgt gcccgtattc ttcgcggcgc gctagcgcgt cgtaaacggt
     8881 tggcggaaaa attcattaat ccgatggggc ggcaaacaga tgctgcgctg ttctccggta
     8941 agcggatgga tgatgaagaa gagattacct acactgcacg cggtgtggct gccgaccctg
     9001 acgacgtcct gttttcgggc aatcgtgcaa cgcagccaga atatgacgaa tacgatccat
     9061 tattaaacgg tgcgccaatt accgaacctg ttgctgtagc agctgctgct accacggcga
     9121 cacaaagctg ggctgcaccg gttgaacctg tgactcagac gccgccagtt gcctctgttg
     9181 atgttccacc tacgcaacct acagtagcct ggcagcctgt accggggccg caaacgggag
     9241 aaccggttat tgcacctgca ccggaaggtt acccacagca gtcacaatat gcgcagcctg
     9301 cagtgcaata taatgagccg ctgcaacaac ctgtacagcc acagcagccg tattatgcgc
     9361 ctgcggctga acaacctgtg caacaacctt actacgctcc tgcggcggaa caacctgttc
     9421 agcaaccgta ttacgcccct gcgccagaac aaccggtggc aggtaacgcc tggcaagccg
     9481 aagagcagca atccactttt gctccacagt ctacatacca gacggagcaa acttatcagc
     9541 agccagctgc tcaggagccg ttgtaccaac agccgcaacc cgttgaacag cagcctgttg
     9601 tggagcctga acccgttgta gaagagacaa aacccacgcg tccgccgctt tactattttg
     9661 aagaagtgga agagaagcga gcccgtgaac gtgaacaact tgcggcctgg tatcaaccga
     9721 ttccagaacc ggttaaagaa ccggaaccga tcaaatcttc gctgaaagca ccttctgttg
     9781 cagcagtacc tccagtagaa gccgctgccg ctgtttctcc gctggcatct ggtgtgaaaa
     9841 aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc agtctggcaa
     9901 atagcggtgg accgcgtccg caggtcaaag aggggattgg cccgcagttg ccacgaccga
     9961 aacgcatccg cgtgccaact cgtcgtgaac tggcgtctta cggtattaag ctgccctcac
    10021 agcgtgcggc agaagaaaaa gcccgtgaag cccagcgtaa tcagtacgat tctggcgatc
    10081 agtacaacga tgatgaaatc gatgcgatgc agcaggatga actggcacgt cagttcgccc
    10141 agacgcagca gcaacgctat ggcgaacagt atcaacatga tgtgcccgtg aacacagaag
    10201 atgcagatgc tgcggcagag gcagaactgg ctcgtcagtt tgctcaaact caacaacaac
    10261 gttattccgg cgaacaaccg gctggggcga atccgttctc gctggatgat tttgaatttt
    10321 cgccaatgaa agcgttgctg gatgatggtc cacacgaacc gctgtttacg ccaattgttg
    10381 aacctgtaca gcagccgcaa caaccggtag caccgcagca gcaatatcag cagccgcaac
    10441 aaccggtagc accgcagcag caatatcagc agccgcaaca accggttgca ccgcagccgc
    10501 agtatcagca gccgcaatat cagcagccac aacagccggt tgcacagcag ccgcaatatc
    10561 agcagccgca acaaccggtt gcaccgcagc agcaatatca gcagccgcaa cagccggttg
    10621 cacagcagcc gcaatatcag cagccgcaac agccagttgc gccgcagccg catgataccc
    10681 tgcttcatcc gctgttgatg cgcaatggtg acagccgtcc gttgaataaa ccgacgacgc
    10741 cgctgccttc tctggatttg ctgacacctc cgccgagcga agtggagccg gtagatacct
    10801 ttgcgcttga acaaatggcg cgcctggtag aagcgcgtct ggctgatttc cgtattaaag
    10861 ccgatgttgt caattactct ccggggccag ttatcactcg ctttgaattg aacctggcac
    10921 cgggcgtaaa agcagcgcgc atttctaact tgtcacgtga ccttgcccgt tcactttcga
    10981 cggtggcggt gcgtgtcgtt gaagttattc ctggcaaacc ctatgtaggt ctggagttac
    11041 cgaataaaaa acgacaaacc gtttatctgc gcgaagtttt ggataacgcc aaattccgcg
    11101 ataatccgtc gccattaacc gtggtgctgg gtaaagatat cgccggtgag ccggtggttg
    11161 ccgatctggc gaaaatgccg cacttgttgg ttgcggggac taccggttcc ggtaagtctg
    11221 tcggggtgaa cgcgatgatc ctgagcatgc tttataaagc acagccagaa gatgtgcgtt
    11281 tcatcatgat cgacccgaaa atgctggagc tttcggttta tgaaggcatt ccgcatctgt
    11341 taacggaagt cgttaccgat atgaaagatg ccgccaacgc gctgcgctgg tgtgttaacg
    11401 agatggagcg tcgttataaa ctgatgtctg cgctgggtgt gcgtaatctg gcgggttata
    11461 acgaaaaaat tgctgaagcc gataggatga tgcgtccgat tccagacccg tactggaagc
    11521 cgggtgacag tatggatgcc cagcatccgg tgctgaaaaa agaaccgtac attgtggtgt
    11581 tggtggacga atttgccgac ctgatgatga cggtaggtaa aaaagtggaa gagctgatag
    11641 cacgtctggc gcaaaaagcc cgtgccgcgg gtatccacct cgtactggca acgcagcgtc
    11701 catcggttga tgttattact ggtctgatta aagcgaatat tccgacccgt atcgccttta
    11761 ccgtatccag taagattgac tcacgtacca ttcttgatca ggctggtgcg gaatcactgc
    11821 tgggtatggg ggatatgctc tactctgggc cgaactccac gttgccggta cgtgtccatg
    11881 gtgcttttgt tcgcgatcag gaagttcatg ccgtggtgca ggactggaaa gcgcgtggtc
    11941 gcccacagta tgttgatggc atcacctccg acagtgaaag cgaaggtggc gtgggcggtt
    12001 tcgatggcgc tgaagaactg gatccgttgt tcgatcaggc ggtgcagttt gtcactgaaa
    12061 aacgcaaagc gtcaatttct ggcgtacagc gtcagttccg cattggttat aaccgtgcag
    12121 cgcgtattat cgaacagatg gaagcgcagg ggattgtcag cgaacagggg cacaacggta
    12181 atcgtgaagt gctggcccca ccgccatttg actaattaat gcaacgtaga ccggataaga
    12241 cgcgatagcg tcgcatccgg tattctatca actgaaaatt cagtattttc ttctttcctc
    12301 aagctgatta ttagcctgga atagagagta gagggaactc ccgattggga gtgacgtaat
    12361 ttgaggaata atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc
    12421 aagcagcgtt tgggctgatg ccgcaagcga tcttaaaagc cgcctggata aagtcagcag
    12481 cttccacgcc agcttcacac agaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg
    12541 tcagggcgat ctgtgggtga aacgtccaaa cttattcaac tggcatatga ctcaacctga
    12601 tgaaagcatt ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca
    12661 agctacggca acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg
    12721 caaccagtcc agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgttct
    12781 gacgccgaaa gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg
    12841 cacaatccat cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcagctgaa
    12901 atcccagcaa aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt
    12961 cacggtagat gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt
    13021 cggataatac ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata
    13081 tcggccagca acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc
    13141 atctgcattc tatgatcctc tgggggccgc cgggtaccgg caaaacaacc ctcgctgaag
    13201 tgattgcccg ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggtgtga
    13261 aagagattcg cgaggcgatc gagcgcgctc ggcaaaaccg caatgcaggt cgccgcacta
    13321 ttctttttgt tgacgaagtt caccgtttca acaaaagcca gcaggatgca tttctgccac
    13381 atattgaaga cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc
    13441 ttaattcggc actgctttcc cgtgcccgtg tctatctgtt gaaatccctg agtacagagg
    13501 atattgagca agtactaact caggcgatgg aagacaaaac ccgtggctat ggtggtcagg
    13561 atattgttct gccagatgaa acacgacgcg ccattgctga actggtgaat ggcgacgcgc
    13621 gccgggcgtt aaatacgctg gaaatgatgg cggatatggc cgaagtcgat gatagcggta
    13681 agcgggtcct gaagcctgaa ttactgaccg aaatcgccgg tgaacgtagc gcccgctttg
    13741 ataacaaagg agatcgcttt tacgatctga tttccgcact gcataagtcg gtacgtggta
    13801 gcgcacccga tgcggcgctg tactggtatg cgcgaattat taccgctggt ggcgatccgt
    13861 tatatgtcgc gcgtcgctgt ctggcgattg cgtctgaaga cgtcggtaat gccgatccac
    13921 gggcgatgca ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag
    13981 gtgaacgcgc cattgctcag gcgattgttt acctggcttg cgcgccaaaa agcaacgctg
    14041 tctacactgc gtttaaagcc gcgctggcgg atgcacgcga acgtccggat tatgacgtgc
    14101 cggttcattt gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat
    14161 atcgttacgc tcatgatgaa gcaaacgctt atgctgccgg tgaggtttat ttcccgccgg
    14221 aaatagcaca aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg
    14281 aaaagctcgc ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat
    14341 gttatcgttg cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc
    14401 ccatttaatt cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc
    14461 cagacgcagt cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg
    14521 gcgctcttga agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc
    14581 gtaactcccg atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt
    14641 tacgtctgga agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg
    14701 ctttacaggc tgaaattcgc gatatcgcgc taaccatccc taacctgcct gcagatgaag
    14761 tgccggtagg taaagacgaa aatgacaacg ttgaagtcag tcgctggggc accccgcgtg
    14821 agtttgactt tgaagttcgt gaccatgtga cgctgggtga aatgcactct ggcctcgact
    14881 ttgcagccgc agttaagttg actggttccc gctttgtcgt catgaaaggg cagattgctc
    14941 gcatgcaccg cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca
    15001 gtgagaacta tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc
    15061 cgaaatttgc tggcgatctg ttccatactc gtccgctgga agaagaagca gacaccagta
    15121 actatgcgct gatcccaacg gcagaagttc cgctgaccaa cctggtacgc ggtgaaatca
    15181 tcgatgaaga tgatctgcca attaagatga ccgcccacac cccatgtttc cgttctgaag
    15241 ctggttcata tggtcgtgac actcgtggtc tgatccgtat gcaccagttc gacaaagttg
    15301 aaatggtgca gatcgtgcgc ccggaagact caatggcggc actggaagag atgaccggtc
    15361 atgcggaaaa agtcctgcag ctgctgggtc tgccgtaccg caaaatcatc ctttgtaccg
    15421 gcgacatggg ctttggtgct tgcaaaactt acgacctgga agtatggatc ccggcacaga
    15481 acacctaccg cgagatctct tcctgctcca acgtctggga tttccaggcg cgtcgtatgc
    15541 aggcacgttg ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt
    15601 ctggtcttgc tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg
    15661 gtcgtattga agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct
    15721 aatacccaat ttttctgaat ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga
    15781 taccgaacaa taattactcc tcacttacaa gtaatactac tttcgagtga aaatctacct
    15841 atctctttga ttttcaaatt attcgatata tacaagccta tatagcgatc tgctatagaa
    15901 ataattacac aatacggttt gttactggaa tcaatcgtga gcaagcttga gtgagccatt
    15961 atgaaaacta aaatccctga tgcggtattg gctgctgagg tgagtcgccg tggtttggta
    16021 aaaacgacag cgatcggcgg cctggcaatg gccagcagcg cattaacatt accttttagt
    16081 cggattgcgc acgctgtcga tagcgctatt ccaacaaaat cagacgaaaa ggttatctgg
    16141 agcgcctgta cagttaactg tggtagtcgc tgcccgctac gtatgcacgt agtggacggt
    16201 gaaatcaaat atgtcgaaac ggacaatacc ggcgatgaca attacgacgg cctgcaccag
    16261 gttcgcgcct gtctgcgtgg gcgttccatg cgtcgccgtg tctacaatcc ggaccgcctg
    16321 aaatatccga tgaaacgagt cggggcgcgc ggtgaaggca aattcgagcg cattagctgg
    16381 gaagaagcct acgacatcat cgcgaccaaa atgcagcgcc tgatcaaaga gtacggcaac
    16441 gagtctatct atctgaacta tggcaccggt acgctgggcg gcaccatgac ccgctcgtgg
    16501 ccgccgggaa ataccctggt cgcgcggcta atgaactgct gcggcggtta tctgaaccat
    16561 tatggcgact actcctccgc gcaaattgcg gaaggtctga actataccta cggcggctgg
    16621 gcagatggta acagcccgtc ggatatcgaa aacagtaaac tggtagtgct gtttggtaat
    16681 aaccctggcg aaacgcgaat gagtggcggt ggggtgactt actatcttga acaggcgcgc
    16741 cagaaatcta atgctcgcat gatcatcatc gatccgcgct ataccgacac cggtgccggg
    16801 cgtgaagatg agtggatccc tattcgtccg ggaacagatg ccgcactggt taacggtctg
    16861 gcgtatgtga tgatcaccga aaacctggta gatcaggaat tcctcgataa atattgcgtt
    16921 ggctacgacg agaaaaccct gccagccagt gcgccgaaaa atggtcacta taaagcttat
    16981 attctgggtg aaggacccga tggcgtggct aaaacaccgg aatgggcttc gcaaattacc
    17041 ggtatcccgg cagataaaat catcaaactt gctcgtgaaa ttggtagcac caaaccggca
    17101 tttatcagcc aggggtgggg gccgcagcgc catgccaacg gcgaaatcgc aacccgtgct
    17161 atctcgatgc tggcgattct gaccggtaac gttggtatta acggggggaa cagcggcgcg
    17221 cgcgaaggtt catacagctt accgtttgtc cgtatgccga ctctcgaaaa cccgatccag
    17281 accagcattt ctatgtttat gtggaccgat gccattgaac gtggcccgga aatgacggcg
    17341 ctgcgtgatg gtgtgcgcgg gaaagataag ctggatgtgc cgatcaaaat gatctggaac
    17401 tatgccggta actgcctgat taatcagcat tctgaaatca accgtaccca tgaaatcctt
    17461 caggatgata agaagtgcga gctgattgtg gttatcgact gccacatgac ctcatcggcg
    17521 aaatatgctg acatcctgct gcctgactgc accgcttccg aacagatgga ctttgcactg
    17581 gatgcatcct gcgggaatat gtcttacgtt attttcaatg atcaggtcat taaaccgcgc
    17641 tttgaatgta agactatcta tgagatgacc agcgaactgg caaaacgtct tggcgtcgaa
    17701 caacagttta ctgaaggtcg tactcaggaa gagtggatgc ggcatctgta tgcccagtcg
    17761 cgggaagcga ttcctgaact gccaacgttt gaagaattcc gtaagcaggg gatctttaaa
    17821 aagcgcgatc cgcaagggca tcacgttgct tacaaagcct tccgtgaaga tccgcaggct
    17881 aatccattga ccacgccatc gggcaaaatt gagatctatt cacaggcgct ggctgacatt
    17941 gccgctacct gggaattgcc agaaggtgat gtgatcgatc cactgccgat ctatacgccg
    18001 ggctttgaaa gttatcagga tccgctgaac aaacagtatc cgctgcagct tacgggtttc
    18061 cactataaat ctcgcgttca ctcaacttac ggcaacgttg atgtgctgaa agcagcttgc
    18121 cgtcaggaaa tgtggatcaa cccgcttgat gcgcaaaaac gcggtatcag caacggcgat
    18181 aaagtcagga tctttaatga tcgtggtgag gttcatattg aggcgaaagt gacgccacga
    18241 atgatgccgg gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga tacaaaacgt
    18301 gtcgataagg gcggttgtat taacgtactg accactcaac gtccgtctcc tcttgccaag
    18361 gggaatccat cacatacgaa ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac
    18421 aacccagtat ggatttttta ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct
    18481 ggcctgtaaa gactacaaag atttgacgcc agaagtcagc ttccgccgca tttatgaata
    18541 tgctggtggc gactggcagg aagataacgg tgtctggcac cagaacgtgt ttgcctacta
    18601 tctgtcgatt tcatgtaacc actgcgaaga tccggcttgt accaaagtct gcccaagcgg
    18661 tgcgatgcat aaacgtgaag atggttttgt cgttgtcgat gaagatgtgt gtattggctg
    18721 tcgttactgc catatggcct gcccgtatgg cgcaccgcag tacaacgcaa ccaaaggtca
    18781 tatgaccaaa tgtgatggtt gctatgaccg cgtcgcagaa ggtaaaaaac cgatctgcgt
    18841 cgaatcttgc ccgctacggg cgctggattt cggacctatc gacgagctgc gtaaaaaaca
    18901 cggcgatctg gcggctgttg cgccgttgcc gcgtgcgcac ttcaccaaac cgaatattgt
    18961 gatcaaacct aacgccaata gccgcccaac cggggatacc actggctatc tggcaaaccc
    19021 gaaggaggtg tgagatggga agtggatggc atgaatggcc gctgatgatc ttcacggttt
    19081 tcgggcaatg tgtagcgggt ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc
    19141 tgcgagcaga agcccagcag cgtgttatcg cctgcatgtt gggtttatgg gtgctgatgg
    19201 gcattggctt tatcgcctct atgcttcatc ttggttcacc gatgcgcgct tttaactcgc
    19261 tcaatcgggt aggggcttca gcactcagta acgaaatcgc cagcggttcg atcttttttg
    19321 ctgtaggcgg catcggctgg ttgctggcaa tgctgaaaaa gctttcaccg gcattgcgta
    19381 cgctttggct ggtggtgacg atggttcttg gcgtagtctt tgtctggatg atggtgcgtg
    19441 tgtataacag catcgataca gtacccacct ggtacagcat ctggacactg atgggcttct
    19501 tcctgacgat gtttatgggc ggcccgttac tgggttacct gttgctgagt ctggctggtg
    19561 tcgatggctg ggcgatgcgt ctgctgccag ccatttctgt tctggcactg gtagtgagtg
    19621 gcgtgatgtc agtgatgcag ggcgcagagc tggcgactat tcatagctct gtgcagcagg
    19681 ccgcagcgct ggtgccggac tatggtgcgc tgatgtcctg gcggattgtg cttttggccg
    19741 ttgccctgtg cttgtggatt gcaccacagc taaaaggtta tcagcctgcg gttccgctac
    19801 tttctgtatc attcattctg cttctggcag gggaattaat cggtcgtggc gtattctatg
    19861 gtttgcatat gaccgtgggg atggctgtcg caagttaatc agtaacaacc ggggtttcgg
    19921 ccccggttcg cttttatttc tgcttcgtta acgtgtcata actggtcatc aagttacgat
    19981 aatcaggaat gtggttagag aacaacgtcg ccagtccttc aatatcatta cgccagtcgc
    20041 gatgcagctc gcacgccacg ccgaaccatg tcatcaattg cgcaccagct tgcgacatgc
    20101 gatcccatgc cgaatgccgg gtaatttcat taaaggtccc tgacgcatca gtcacgacaa
    20161 agacgtcaaa cccttcttca atggctgaaa gcgccgggaa tgccacgcaa acttcggtta
    20221 ccacaccggc aataattaac tgttttttac ctgtcgcttt gacagctttt acaaaatctt
    20281 cgttatccca ggcgttaata tttcccgggc gagcaatgta aggtgcatcg gggaattgtg
    20341 ctttcagttc tggaactaat gggccgttag gaccggtttc aaaactggtg gtaagaatag
    20401 ttggtaagtt gaaatactta gccaggtcac ccagcgccag cacgttattt ttaaacttat
    20461 cgggttcgat atcccgtaca agggaaagta aaccagcctg gtgatcaaca agcaaaacgg
    20521 cagcatcatt tttatcaaga cgaacatacg gtttggtcat cctcttctcc tttcgagagt
    20581 cagtggtacg tctaaatcgg tcagacgtga tgcattgctc tgaaagcata gacgggaaat
    20641 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    20701 atcaataaaa acaatcagat gagattaatc gtctcttcag cattggcgtt atgaatgagt
    20761 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    20821 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    20881 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgattg
    20941 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtct acatggcagg
    21001 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    21061 atgtcattaa acgcattggc tttaaccgta gctattatct ggcctccttc atttttgccg
    21121 ctggctgtgc cggccttggc ctgatgattg gattctggag ttggttggcg tggcgttttg
    21181 tcgctggcat cggctgtgcc atgatttggg tggttgttga gagtgcgctg atgtgcagtg
    21241 ggacgtcgcg taaccgcggg cggttgcttg ctgcgtatat gatggtttat tacgtcggaa
    21301 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gttgatgagt gtattgccgt
    21361 gggttacagg tttgacgttg gcaggaatct taccgctgtt gtttacgcat gtcctgaatc
    21421 agcaggctga aaaccatgat tcgacgtcaa ttacgtcaat gctaaaactc cgtcaggcac
    21481 ggcttggcgt gaatggctgc attatctcag gaatcgtgct gggatctcta tatggcctga
    21541 tgccgctgta cctcaatcac aaaggggtga gcaatgccag cattggtttc tggatggcgg
    21601 tactggtcag tgcgggtatc ctcgggcaat ggccgattgg acgtctggcg gataagtttg
    21661 gtcgactgct ggtgttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc
    21721 ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat
    21781 atccggtggc gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtagcgatga
    21841 accaggcctt actgttgagc tataccgtgg gaagcctgct tggcccgtca tttaccgcta
    21901 tgctaatgca gaatttctcc gataatttat tatttatcat gatcgccagc gtatcgttta
    21961 tctatttgct gatgctgctt cgtaacgccg gtcatacgcc gaaacccgtg gctcacgtgt
    22021 aaatgaattc aagcagagtg tgaacttact gtttcacact ctgcttttct gtgcacaaaa
    22081 aagcggcaac ttaataaagt tgccgcttta cggggaaatt agaacatgac cttatgaccg
    22141 tactgctcaa gaatgccttt cacgcgttcc atggtctctt tcttcggtgg tttaacacca
    22201 tcgagtttgt attcttcacc cattgccacc catttgtgtt tgcccagttc gtggtagggg
    22261 aggagctcga ttttctcaac gttgcccata tcacgggtaa attcaccaag gcgatgcgct
    22321 gaatcgtcat cgtcagacca gcctgggaca acaacatagc ggatccacac cttcacattt
    22381 ttgttcgcca gatatttagc gaactccagc gtgcggtggt tggaaactcc aaccagattt
    22441 tggtggatct cgtcgttcat ctgtttgaga tcgagcatta ccaggtcggt tacttccagc
    22501 agttcatcaa tcaccggatc gtaacgacga acaaaaccgt tggtgtccag acaggtatga
    22561 atgccttctt ttttgcaggc gcggaaccag tcacgaacaa actcagcttg taggattgcc
    22621 tcaccgccgg atgcggtaac gccgccgccg gaagcgttca taaagtggcg ataggtcacc
    22681 acttccttca tcaaatcttc aacggtaact tctttaccgc catgcgtatc ccaggtgtcg
    22741 cggttatgac aatacaggca gcgcatcagg cagccctgga aaaaggtgat aaagcgaata
    22801 cccgggccgt ctacggttcc acaggattca aaggagtgaa tgcgaccaat aactgacatt
    22861 gcggtgtttc tccagatgtg gcccatctga ggccgtgtgg tgcgcagctc gaaggctacg
    22921 tcgagtctgt tttggcagtc accttaaagt atagatagct gacaaaaaag gctctcgtgc
    22981 taaaaaaggc cccactttcg tggagccttt attgtacgct ttttactgta cgatttcagt
    23041 caaatctaat tacatagatt gagtgaaggt acgagtaata acgtcctgct gctgttcttt
    23101 agtcagcgag ttgaaacgta ctgcgtagcc agatacacgg atggtcagct gcggatattt
    23161 ttccgggttt tccatcgcgt cgagcagcat ttcacggttc atcacgttaa cgttcaggtg
    23221 ctgaccacct tcgatggatg cttcgtggtg gaagtaacca tccatcagac cagccaggtt
    23281 ggtcttacga acttcgtcgt ctttacccag tgcgttcgga acgatagaga aggtgtagga
    23341 gataccatct ttagcgtaag cgaacggcag tttagcaacg gaagtcagag acgctacagc
    23401 acctttctgg tcacgaccgt gcatcgggtt agcacccggt ccgaacggtg cgccagcacg
    23461 acgaccgtct ggggtgttac cagttttctt accatacaca acgttagaag tgatggtcag
    23521 aacagactga gtcgggatag cgtcacggta ggtgtgcagt ttctgaattt tcttcatgaa
    23581 acgttctacc aggtcaacag ccaggtcatc tacacgcgga tcgttgttac caaactgcgg
    23641 gtattcgcct tcgatttcga agtcgatagc cagaccgtct tcgtcacgaa tcggtttaac
    23701 tttcgcatat ttgattgcag acagggagtc agcagcaacg gacagaccag cgataccaca
    23761 cgccatggtg cggataacgt cacggtcgtg cagcgccatc agagaggctt cgtagctgta
    23821 cttgtcgtgc atgtagtgga tgatgttcag tgcagtgatg tactgtttag ccagccagtc
    23881 catgaagtga tccatgcgct ccatcacttc atcatagttc aggacatcgc ctttgatcgg
    23941 ttcagacttc ggaccaacct gcattttcag tttttcgtca acgccgccgt tgattgcgta
    24001 cagcatggtt ttcgccaggt ttgcacgcgc accgaagaac tgcatttgtt taccaacgat
    24061 catcgggctt acgcagcaag caatagcgta gtcatcgttg ttgaagtccg gacgcatcag
    24121 gtcatcgttc tcatactgca gagaagaggt gtcgatggac actttagcgg cgaatttctt
    24181 gaagttcagc ggcagttttt cagaccacag aatggtcatg ttcggttccg gagacggacc
    24241 catggtgtac agggtgttca ggaaacggaa gctgtttttg gtaaccaggg tacgaccgtc
    24301 gaggcccata ccaccgatag attcggttgc ccagatcggg tcgccagaga acagttcatc
    24361 gtattccgga gtacgcagga agcgaaccat acgcagtttc atgaccaggt ggtcaaccat
    24421 ttcctgcgct tcttgttcgg tgatcttgcc agctttcagg tcacgttcga tgtacacatc
    24481 caggaaggtg gaggtacgac cgaaggacat tgcagcaccg ttctgagact taacagcagc
    24541 caggtagccg aagtaagtcc actggatagc ttcctgagcg ttagtagccg gaccagagat
    24601 gtcgtagccg tatttcgcag ccatttcttt catctgaccc agagcgcggt gctgttcagc
    24661 gatttcttcg cgcagacgga tagtctgttc caggtttacg ccgttttcca gatcagcctg
    24721 cagagaggtg aactgtgcaa gtttgtcttt catcaggtag tcgataccgt acagcgcaac
    24781 gcgacggtag tcaccgatga tacgaccacg gccataagca tctggcagac cggtcagaac
    24841 accggattta cggcaacgca ggatgtccgg agtgtaaacg tcgaacacgc cctggttgtg
    24901 agttttacgg tattcagtga agattttttt gatcatcggg tccagttcgc ggttgtacgc
    24961 tttgcaggaa ccttcgatca ttttgatacc accgaacggg ataagagcac gtttcagcgg
    25021 agcttcagtc tgcagaccaa cgattttctc aagctgcttg ttgatgtaac cagcgtcgtg
    25081 agaggtgatg gtggaagcaa cagcggtgtc aaagtcaact ggcgcgtgag tgcggttttc
    25141 cagtttaacg ccttccatta ctttgtccca cagggtggtg gtcgcttcag tagcgccagc
    25201 caggaaggac tcgtcaccct cgtacggagt gtagtttttc tgaatgaagt cacggacgtt
    25261 tacttcattc tgccagtcac ctttggtaaa accttcccag gctgtggcta acttttcatt
    25321 aagctcggac atgtaacacc taccttctta agtggatttt ttatttactg cgtacttcga
    25381 caaccattaa tggtgatcgt tttcacgcag gtaaatgacc cagtatgtca acccaaccaa
    25441 caaaccaccg ccgataatat taccgatcgt aaccggaatc aggttatcag tgatgaaatt
    25501 catcacggtc aggtgagaaa aattttccgg tgcagaaccg acagcggtcc agaattccgg
    25561 agatgcgaag tcgcggatta caatacccat cgggatcata aacatgtttg cgatactgtg
    25621 ctcaaaaccg ctggcaacaa acatcgcgac cggcagcacc ataatgaacg ctttgtccat
    25681 caggctgcgg ccagaataac tcatccatac tgccagacat accatcaggt ttgccaggat
    25741 accaagacag acggcctcaa taaaagtatg gtgcactttg tggtcggcgg tttgtaggac
    25801 gtttagtccc cattgaccat ttgcggtcat atactcgccg gaaagccaca ttaaaagtac
    25861 aaacagcagt gcgccgacca ggttgccaaa ataaacattt agccagtttt tcgccaactg
    25921 accccaggtg atgcgcccac tcgccttggc aacaacaatc aacactgtgg aagtaaagag
    25981 atcggctccg cagacaacac aaagaatcaa ccccagagag aagcaaatgc cgccaaccag
    26041 ttttgccatg ccgaagggca ttgtacctgt gccagtggtt gctgtgatat agaagacgaa
    26101 tgcgattgag atgaaaacac cggcggtaat cgccagatag aaagtcttaa gcggatgttt
    26161 cgttgcttta tagacacctg cctcttcggc cactttggcc attgcagcag gaagtaaaag
    26221 atcaaaaggg ttgtcagctt tcacactaac tctctcttta ttaagtcggc gacgagatac
    26281 taacaaagca ttatagatga gaaattgata tagatcatat ctcgcctggc ttataggc
//
LOCUS       DADSZD010000055        26251 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.055, whole genome shotgun sequence.
ACCESSION   DADSZD010000055 DADSZD010000000
VERSION     DADSZD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.055"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(73..1275)
                     /gene="ubiI"
                     /locus_tag="J1Z28_002010"
                     /gene_synonym="visC"
     CDS             complement(73..1275)
                     /gene="ubiI"
                     /locus_tag="J1Z28_002010"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBA7716847.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQEILSRRASCYHGMEVWDKDSFGHISFDDQSMGYN
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQAGEDEFNRALNIAFDNRLGLCKVESERLVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1298..2476)
                     /gene="ubiH"
                     /locus_tag="J1Z28_002011"
                     /gene_synonym="visB"
     CDS             complement(1298..2476)
                     /gene="ubiH"
                     /locus_tag="J1Z28_002011"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBA7716848.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSLVEVTLESGETLTGCVLVA
                     ADGTHSALATVCGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTKHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2473..3798)
                     /gene="pepP"
                     /locus_tag="J1Z28_002012"
     CDS             complement(2473..3798)
                     /gene="pepP"
                     /locus_tag="J1Z28_002012"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBA7716849.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPMVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQERSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3824..4402)
                     /gene="ygfB"
                     /locus_tag="J1Z28_002013"
     CDS             complement(3824..4402)
                     /gene="ygfB"
                     /locus_tag="J1Z28_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBA7716850.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4570..4899
                     /gene="zapA"
                     /locus_tag="J1Z28_002014"
     CDS             4570..4899
                     /gene="zapA"
                     /locus_tag="J1Z28_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBA7716851.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4941..5124
                     /gene="ssrS"
                     /locus_tag="J1Z28_002015"
     ncRNA           4941..5124
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="J1Z28_002015"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5145..5747
                     /locus_tag="J1Z28_002016"
     CDS             5145..5747
                     /locus_tag="J1Z28_002016"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBA7716852.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRTLTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5848..5907)
                     /gene="ibsC"
                     /locus_tag="J1Z28_002017"
     CDS             complement(5848..5907)
                     /gene="ibsC"
                     /locus_tag="J1Z28_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBA7716853.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(6137..7369)
                     /gene="serA"
                     /locus_tag="J1Z28_002018"
     CDS             complement(6137..7369)
                     /gene="serA"
                     /locus_tag="J1Z28_002018"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBA7716854.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGTKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(7625..8284)
                     /gene="rpiA"
                     /locus_tag="J1Z28_002019"
     CDS             complement(7625..8284)
                     /gene="rpiA"
                     /locus_tag="J1Z28_002019"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBA7716855.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFINALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8340..8570)
                     /locus_tag="J1Z28_002020"
     CDS             complement(8340..8570)
                     /locus_tag="J1Z28_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716856.1"
                     /translation="MHFAQRVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALLAGRKGMPAIARYFMDELTTRLANGV"
     gene            8711..9604
                     /locus_tag="J1Z28_002021"
     CDS             8711..9604
                     /locus_tag="J1Z28_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HBA7716857.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            9807..10862
                     /locus_tag="J1Z28_002022"
     CDS             9807..10862
                     /locus_tag="J1Z28_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBA7716858.1"
                     /translation="MSNVQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAHRGYD
                     SDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIVAAEE
                     QGVTSDKLTGTIQNDILKEFLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISS
                     YHMGEAGANCVQQVAFTLANRIEYIKAAISAGLKIDGFVPRLSFFFGIGMDLFMNAAM
                     LRAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMCRDYLHRYLENASGGHIHHDLS
                     HVFDLHRNLIATGSMLG"
     gene            complement(10859..11755)
                     /locus_tag="J1Z28_002023"
     CDS             complement(10859..11755)
                     /locus_tag="J1Z28_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716859.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASRFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSRDIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSANGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISDVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(11913..12653)
                     /locus_tag="J1Z28_002024"
     CDS             complement(11913..12653)
                     /locus_tag="J1Z28_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBA7716860.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            complement(12746..13381)
                     /gene="argO"
                     /locus_tag="J1Z28_002025"
     CDS             complement(12746..13381)
                     /gene="argO"
                     /locus_tag="J1Z28_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBA7716861.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(13520..14380)
                     /locus_tag="J1Z28_002026"
     CDS             complement(13520..14380)
                     /locus_tag="J1Z28_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBA7716862.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(14571..15650)
                     /gene="fbaA"
                     /locus_tag="J1Z28_002027"
     CDS             complement(14571..15650)
                     /gene="fbaA"
                     /locus_tag="J1Z28_002027"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBA7716863.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(15865..17028)
                     /gene="pgk"
                     /locus_tag="J1Z28_002028"
     CDS             complement(15865..17028)
                     /gene="pgk"
                     /locus_tag="J1Z28_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBA7716864.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(17078..18097)
                     /gene="epd"
                     /locus_tag="J1Z28_002029"
     CDS             complement(17078..18097)
                     /gene="epd"
                     /locus_tag="J1Z28_002029"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBA7716865.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            18469..18900
                     /locus_tag="J1Z28_002030"
     CDS             18469..18900
                     /locus_tag="J1Z28_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA7716866.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            18923..19501
                     /locus_tag="J1Z28_002031"
     CDS             18923..19501
                     /locus_tag="J1Z28_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBA7716867.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            19502..20209
                     /locus_tag="J1Z28_002032"
     CDS             19502..20209
                     /locus_tag="J1Z28_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBA7716868.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     TMILLMLAEFGAWIWLR"
     gene            20197..20874
                     /locus_tag="J1Z28_002033"
     CDS             20197..20874
                     /locus_tag="J1Z28_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBA7716869.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQAREMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            20868..21545
                     /locus_tag="J1Z28_002034"
     CDS             20868..21545
                     /locus_tag="J1Z28_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA7716870.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVASLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRMVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(21517..22230)
                     /locus_tag="J1Z28_002035"
     CDS             complement(21517..22230)
                     /locus_tag="J1Z28_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBA7716871.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRQVLEES
                     LPANLTLMMTAMGEYRLMD"
     gene            complement(22227..22736)
                     /gene="fumE"
                     /locus_tag="J1Z28_002036"
     CDS             complement(22227..22736)
                     /gene="fumE"
                     /locus_tag="J1Z28_002036"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBA7716872.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(22758..23073)
                     /locus_tag="J1Z28_002037"
                     /pseudo
     CDS             complement(22758..23073)
                     /locus_tag="J1Z28_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(23070..24347)
                     /locus_tag="J1Z28_002038"
     CDS             complement(23070..24347)
                     /locus_tag="J1Z28_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA7716873.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPARVVVVDIDDKRLTQV
                     QKLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(24362..25750)
                     /locus_tag="J1Z28_002039"
     CDS             complement(24362..25750)
                     /locus_tag="J1Z28_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBA7716874.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(25778..26221)
                     /gene="cmtB"
                     /locus_tag="J1Z28_002040"
     CDS             complement(25778..26221)
                     /gene="cmtB"
                     /locus_tag="J1Z28_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBA7716875.1"
                     /translation="MRLIDYFPELSISVIHSTKDWQEAINFSMASLLAKNYISENYIQ
                     AIKDSTISNGPYYILAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
BASE COUNT         6413 a         7044 c         6429 g         6365 t
ORIGIN      
        1 gaaaatctcc cggcgaaaaa accgggagat gaaagtgtga tgggtatcaa ataaacagca
       61 gaagagaaat atttaacgca gccattcagg caaatcgttt aatcccattg cctgacgaat
      121 aagttgtggc ttaacgccag gaagcgtatc agccagtttc agaccaatat cacgcagcaa
      181 ttttttcgcc ggattggtac cggaaaacag atcgcggaat ccctgcatac cagccagcat
      241 cagcgccgca ctgtgcttac ggctacgctc atagcgacgc aggtaaatgt actgcccgat
      301 gtctttaccc tgacgatgca accgtttcag ttcagcaatc agctctgcag catccataaa
      361 gccgagattt accccctgcc ccgccagcgg gtgaatagta tgcgcggcgt cgcccaccag
      421 cgccagacgg tgtgcggcaa actggcgcgc ataacgcccc gttagcggga acaccaggcg
      481 ctcactttca accttgcata agcccaggcg attatcaaaa gcgatattta acgcgcgatt
      541 aaattcgtct tcgcctgcct gctgcatccg ctgcgcttcc tctggcgaca gtgaccagac
      601 aatcgagcaa agatgcggat cgctaagcgg caaaaaggcc agaatgcctt cgccatgaaa
      661 aacctgccgc gccaccgcat catgcggttc ttccgtgcga atggtcgcca ccagcgcgtg
      721 atgctgataa tcccagaaag tcagcggaat atcggctttg ttgcgcaacc aggaattagc
      781 gccgtccgcg ccaatcacca gacgcgccgt taacatgctg ccatctttca acgtcaggaa
      841 ggtttcattt tctccccagg cgacctgctg taattctgcg ggggccaaca gagtgatatc
      901 tgacgactgc tgcgctttgt tccacagcgc gtagtgaatc actgagtttt caacgatatg
      961 cccaagatgg ttatagccca tgctttgatc gtcaaacgag atgtgaccaa aactgtcttt
     1021 gtcccacact tccataccgt gatagcaact ggctctgcgg gagagaatct cctgccagac
     1081 gccaagacga gtcagtaatt tttcgctggc ggcattgata gccgaaacgc gcagttgtgg
     1141 tggtgcatcc gccgccagag gttccggtac gcgctgctcc agtacggcaa cgcgtaagcc
     1201 gctaccctgt aagccgcagg caaccgccag cccgaccatg ccgccgccaa caatggctac
     1261 atcaacactt tgcattgttt attccttaaa accgccttca acgcgccacc caaccaagag
     1321 tgcgctgcgc cagcacatcg cgtgccgggg taaataattc catcgtcatc agcccgatgt
     1381 tgcgcccgac aaccagcggc gtccaacggt tggcaaaaag atgtacaagg ctgtccgtga
     1441 cgccgatggt tgcttcgcga tcgctctgtc gacgctgctg ataacggcac aatacgccgt
     1501 aatcacccat gtcttctccg cgttcctgcg cctgagtcag ggtttccgca aggctcatga
     1561 catcacgcat accgaggtta aatccttgcc cggcgatggg atgcagagtt tgcgccgcat
     1621 tgcccaccag cacggtacga tgggtaatag gtttggcggc gcgggttagc gctagcggat
     1681 aagcactgcg tttaccggca tgagtaattt tcccaagtcg ccagccaaag gccgactgga
     1741 gttcacggca aaacttctcg tcgctccacg acagcacctc ttcgcgccgt tccagtggat
     1801 gacaccagac cagcgaacag cgtccgtcag acatcggcaa catcgccagc gggccatgtt
     1861 tcgtgaagcg ttcaaaagcg cggccttcgt gcgcaacgga agtcgcaacg ttagcaatca
     1921 cagccagttg ttcgtaaggc tcctgctgcc agtcaacgcc gcacacagtg gctaacgctg
     1981 aatgggtgcc atcagcggct accagcacgc agcccgtcag cgtctcgcca ctctccagcg
     2041 tcacttcaac gaggctctga gtacgggcaa cgttagccac gcgatcaggg caatgcagcg
     2101 ttacgccagg tgctttgcgc agcaatgcaa acagccgttg cccgacattg tgcaattcga
     2161 caacctgccc cagcgccgcc agttggtaat cttctgcggc gagggtgaca aatccagcgt
     2221 gaccacgatc gctgacatgc actgtggtga tggcagttgc gcaatcagcc agagattgcc
     2281 agacgccgat gcgcgccagt tgctgacaag tacccgccgc cagcgcaata gcccgtccat
     2341 caaagcccgg atgagcatgt gactctggcg cagtcgcttc aatcaaatgt accggcagcg
     2401 ccccgtgact taaccgggaa atagccagcg ccagcgtcgc gcccgccatg ccgccaccga
     2461 cgatgattac gctcattgct ttctcgcagc agccatcaac gcttcgattt cttccggctt
     2521 tttcaccacg ctggcggtga ggttttcgtt gccggtttcg gtaatcacaa tgtcgtcttc
     2581 aatacgaatg ccgataccgc gatactgttc tggcacgtct gcatccggcg caatataaag
     2641 acctggctct acggtcagaa ccatgcccgg ttcaagaatg cgcgagcgct cctggccata
     2701 aacgccaacg tcatgaacat ccagacctaa ccagtggcta aggccatgca taaagaaagg
     2761 acgatgggca ttctgagcga tcagttcatc aacatcacct ttcaggatgc cgagttttac
     2821 caggccgcta accatgatgc gcaccacttc accagtgact tcctgaatgg aagttcccgg
     2881 acgatacagg cgcaagctgg tttcgagaga ttccagcaca atatcgtaga tttcacgctg
     2941 ggcctgggtg aatttgccgt tgactgggaa ggttcgggta atatcacccg cgtaaccttt
     3001 gtattcgcaa cccgcgtcaa tcaacaccag atcgccgtcg cgcagttcac actcgttttc
     3061 ggtgtagtgc aaaatgcagc cgttttcacc gctgccgaca atggtgttat aggaagggta
     3121 acgcgcaccg tggcggttaa attcgtggtg aatttcgcct tccagatggt actcgaacat
     3181 ccccggacgg catttttcca tcgcccgtgt atgtgccagg gcggtgattt ctcccgcgcg
     3241 gcggagtacg gcaatctctt ctggcgattt gaacaggcgc atttcatgga ccatgggacg
     3301 ccagtcgatc atcgttgctg gcgcggtgag attttgtcgc gagcctttac gcagtttttc
     3361 cagtgcactg ttcacgattt catcagcata tgcatattcg ccctgagcat ggtaaaccac
     3421 atccaggccg ttaagtagtt gataaagttg ctgattgatt tcgctgaatg ccagtgcgcg
     3481 gtcaacaccc agtttctctg gcgcggcatc ctggcctaag cgacgaccaa accagatctc
     3541 tgccgtcagg tcgcgaacgc ggttaaacaa aacgctgtgg ttatgagtgt cgtcgctttt
     3601 aatcagcacc agcaccgctt ccggttcgtt aaagccggtg aagtaccaga agtcactgtt
     3661 ctgacgatag gggtattcgc tgtcggcgct acgtgttact tctggtgcag caaaaatcag
     3721 cgcggcgctg ccgggttgca tttgctctac cagggcctga cggcgacgct gaaattcttg
     3781 ccgggatata tcactcataa cactctcctt acgttttttg tttttagtgt agagtcggtt
     3841 tttgtacttc tggcgcagtc ggttgcggat gagtaaaggt gtcgtggcat aacagcgcgg
     3901 caacacggac gtactcgatg atctcttcaa gcgacatttc aagctcttcc tgatcttcgt
     3961 cttcgtcgta acccaactgt gcgatgttac gcagatcgtc gatggcttca ccggtttcgc
     4021 cggtcacttt gtccagcttc ggttgcgtaa cgccaagacc aagcaggaag tgattaaccc
     4081 aacctgccag cgcatcagcc cgatcgaaaa cgctgacgtc atcgccatca ggcagataaa
     4141 gctgaaaaag gaagccgtca tcctgcaggg catcgctggt ggcagagtgc attttgcgca
     4201 gtgcctgtgc cagctcatga ccgaaagcca tgccttcgtt cgtcaggtcg tgaagtagcg
     4261 gcagccatga gctgtcatcg ttaccgccac atatcatccc actgattaaa ccatgcatct
     4321 cagcaggggt cagacccgtc ccttgttggt tcagatactg gttcatttcg ttgtaaccag
     4381 gcatttcgtt ctgtatagac ataagcattc gtcgtcaaag ggaggaatat tcatgatatg
     4441 ctaccacttt gggccctggt ggaccagaaa agggcttgtc tcttctcatc agggtagcta
     4501 tagtgtcgcc ccttcgcaga ccatgggtct aaagacgaag gcagcgcagt caatcagcag
     4561 gaaggtggca tgtctgcaca acccgtcgat atccaaattt ttggccgttc actgcgtgtg
     4621 aactgcccgc ctgaccaaag ggatgcgttg aatcaggcag cggacgatct gaaccaacgg
     4681 ttgcaagatc tgaaagaacg cactagagtc acaaatactg aacagttggt cttcattgcc
     4741 gcattgaata tcagctatga gttagcgcaa gaaaaagcga agactcgtga ctacgcggca
     4801 agtatggaac agcgtattcg gatgctgcag cagaccatag aacaagcgtt acttgaacaa
     4861 ggtcgcatca ccgaaaaaac taaccaaaac tttgaatgac acttttcggt ttactgtggt
     4921 agagtaaccg tgaagacaaa atttctctga gatgttcgca agcgggccag tcccctgagc
     4981 cgatatttca taccacaaga atgtggcgct ccgcggttgg tgagcatgct cggtccgtcc
     5041 gagaagcctt aaaactgcga cgacacattc accttgaacc aagggttcaa gggttacagc
     5101 ctgcggcggc atctcggaga ttcccttctt atctggcacc agccatgacg caactaccag
     5161 aactcccact gacattatcc cgacaagaaa tccgcaaaat gattcggcaa cgtcgtcgta
     5221 cgttaacgcc ggaacaacag caggaaatgg gtcaacaagc cgctacccgg atgatgactt
     5281 atcccccggt ggtgatggca catacggtcg ctgtattcct ctcttttgat ggcgaactcg
     5341 acacccagcc actcatagaa caactctggc gcgccggtaa gcgcgtatat cttccagttt
     5401 tgcatccctt tagtgccggt aacttgctgt tcctgaatta ccatccgcaa agcgaactgg
     5461 tgatgaacag gttgaagatc catgagccaa aactggatgt gcgtgacgtg ctaccccttt
     5521 cccgattaga cgtgttgatc acaccgctgg tcgcctttga tgagtacggt cagcgcctgg
     5581 gaatgggcgg tggtttttat gatcggacct tacaaaactg gcagcactat aaaatgcaac
     5641 cggtgggtta tgcgcatgat tgtcagttgg tggaaaaact ccccgttgaa gagtgggata
     5701 tccctcttcc tgcggtggtt acaccgtcga aagtctggga gtggtaaggg cgacacacct
     5761 gcatcgccct gattgacatc gttgattctt taacctaatt tagtgagtaa gggtaaggga
     5821 ggatgactcc tcccctgaga ctgactgtta ataagctggg aaacttatga gtaacagtac
     5881 aatcagtatg atgacaagtc gcatcataac ccttctcctt caagccctcg cttcggtgag
     5941 ggctttaccg ttacagcccc atgctgccct gccatcgtaa atccccatta aataaacaca
     6001 acgcattgat ctgactttga tttattttct ggagcagact cgcaatgtag aatgcgcaat
     6061 gcggcaacgg tgtggagaag ggataaaaaa aacgggcaag tcagtgacct gcccgttgat
     6121 tttcagagaa ggggatttag tacagcagac gagcgcgaat ggtacccgga atagctttca
     6181 ttgcctgcag cgctttttcg gcaacgtctt cgtcggcttc aatatcaata actacataac
     6241 ccatctgggc ggaagtttgc agatattgcg cggcgatgtt gacgccctgc tcggcaaaaa
     6301 ttttgttgag cgcagtcagc acacccggac ggttttcgtg gatgtgcatc agacgacgac
     6361 caccgtgcag tggcagcgag acttccggga agttcaccgc agagagcgtt gaaccattat
     6421 cagaatactt gatcaattta cccgcaactt ccagaccgat attctcctgc gcttcctgag
     6481 tcgaaccgcc aatgtgtggc gtcagaagga cattgtcgaa ttcacacagc ggagaggtaa
     6541 atggatcgct attggtcgcc ggttccgtcg ggaatacgtc gattgccgcc cccgccagat
     6601 gtttgctcgc cagcgcgtca cacagcgctg gaatatccac cacagtaccg cgcgaagcat
     6661 taatcagcag cgagccgggt ttcattagcg aaatctcttt cgtgcccatc atatttttgg
     6721 tggacggatt ctctggtaca tgcagactca ccacatcgct catattcagc aggtcagaaa
     6781 gatgctgtac ctgagtggcg ttgcccagcg gcagtttgtt ttcaatatca taaaagtaaa
     6841 catacattcc cagcgattca gccagaatgc ccaattgcgt accaatatga ccgtagccga
     6901 tgatacccag ctttttgccg cgcgcttcaa aagaacccgc cgccagtttg ttccacacgc
     6961 cacgatgcgc tttagcattg gcttccggca cgccgcgcaa tagcagcagc agttcgccaa
     7021 tcaccagctc cgcaacagag cgcgtatttg agaacggtgc gttaaatacc gggatcccgc
     7081 gctttgccgc cgcatccaga tcaacctggt ttgttcctat acagaaacag ccaatagcga
     7141 ccagtttttc tgcggcgttg atcacgtctt cagtcagatg ggtacgggat cgcaggccga
     7201 tgaagtgggc atcgcggatg gattctttta attgttcatc atccagcgcg cctttgtgaa
     7261 attcgatgtt ggtgtaacca gctgcacgaa ggctttccag cgccttttgg tgcacgcctt
     7321 ctaccagcag aaacttaatc ttgtctttct ccagcgatac ctttgccatt tacccaatcc
     7381 tgtcttttga aatgttgtgt gcggatttgc atccgccttt caacatatca aaaaataata
     7441 ttgcggcaat atgaacgttt gcgtcgcgat gttgaggaaa tatcacgcaa aagtatttct
     7501 tggatgaata tgctggttta gaagatttat ttagagcaat cgacaattgc caggtaaaaa
     7561 cgtgacacat gtcactaaat ttaatgaaga gaattttttt aacgggggag gttcccccgt
     7621 cagatcattt cacaatagtt ttgacaccgt caggtgtgcc aatcagcgca acgtccgcgc
     7681 cacggttagc aaacaaacca acagtcacca cgccaggaat cgcatttatg gcgttttcca
     7741 tcgctatcgg gtcaaggatt tccatgccgt ggacgtcgag gatcacgttg ccgttatcgg
     7801 tcactacgcc ctgacggtat tccggacgac cgcccagttt caccagctgg cgcgccactg
     7861 cactacgtgc catcgggata acttctactg gcagcgggaa tttgcccaga atatcaacct
     7921 gcttggaagc gtctgcaata cagataaatt tttccgcaac cgaagcaatg attttttcac
     7981 gggtcagcgc cgcgccgccg cctttgatca tttgcatgtg gccgttgatt tcatctgcgc
     8041 catcaacgta gatgccaagg ctgtcgactt cgttgagatc aaaaacgtga atgccgaggc
     8101 ttttcagttt ttcagtggaa gcatctgaac tggaaacggc cccttcaatc tggcctttca
     8161 ttgtaccgag tgcgttaata aagtgtgcgg cggtggaacc tgtacctaca ccaacaatgg
     8221 tgccgggctg aacatactga agtgccgccc atcctactgc ttttttcaat tcatcctgcg
     8281 tcatgatcgt ttcgcctgtg gtatgaaatt tcacacgcat tatatacaaa aaaagcgatt
     8341 cagaccccgt tggcaagccg cgtggttaac tcatccataa aatatcgcgc aatggcgggc
     8401 atcccctttc gccccgcaag taaagcatac aacggtctgg gtatgccgct ccacggtgca
     8461 aacaggcgca ccagttcccc gttcgcaagc ccctgtttac aggcaaactg aggcaataac
     8521 gccacgccat tcaacacaac cagggcgcga actcgctgag cgaaatgcat tagcgcaaat
     8581 ccctgatctt ttaagctgcg cgttaaccac tgatacaaat gttacccacc aacggcttcg
     8641 gtatatgcaa cccgacacaa aattgtgtca tagtgcagga aaaagcattt accaggagca
     8701 gacaacagca atgaaacgcc cggactacag aacattacag gcactggatg cggtgatacg
     8761 tgaacgagga tttgagcgcg cggcacaaaa gctgtgcatt acacaatcag ccgtctcaca
     8821 gcgcattaag caactggaaa atatgttcgg gcagccgttg ttagtgcgta ccgtaccgcc
     8881 gcgcccgacg gaacaagggc aaaaactgtt ggcgctacta cgccaggtgg agctgctgga
     8941 agaagagtgg ctgggcgatg aacaaaccgg ttcgactccg ctgctgcttt cactggcggt
     9001 caacgccgac agtctggcga cgtggttgct tccggcactg gctcctgtgt tggctgattc
     9061 gcccatccgc ctcaacttgc aggtagaaga tgaaacccgc actcaggaac gtctgcgtcg
     9121 cggcgaagtg gtcggcgcag tgagtattca acatcaggcg ctgccgagtt gtcttgtcga
     9181 taaactcggt gcgctcgact atctgttcgt cagctcaaaa ccgtttgccg aaaaatattt
     9241 ccctaacggc gtaacgcgtt cggcattact gaaagcgcca gtggtcgcgt ttgaccatct
     9301 tgacgatatg caccaggcct ttttgcagca aaatttcgat ctgcctcctg gcagcgtacc
     9361 ctgccatatc gttaattctt cggaagcgtt cgtgcaactt gctcgtcagg gcaccacctg
     9421 ctgtatgatc ccgcacctgc aaatcgagaa agagctggcc agcggcgaac tgattgactt
     9481 aacgccaggg ctattccaac gccggatgct ctactggcac cgctttgctc ctgaaagccg
     9541 catgatgcgt aaagtcactg atgcgttgct cgattatggt cacaaagttc ttcgtcagga
     9601 ttaatccatc aaataatgcc tgatagcaca tatcaggcgt tatcctcact tctttttgta
     9661 ttccttgaat cacattacaa aatagacaaa tctcaggcgg caaaaaacga catctgaatg
     9721 catttttttg ctggcgacaa acccacgtaa aaagctcacc gtaagcgcaa ataccctcat
     9781 tttgattgcg ttttacggag caaataatgt ctaacgtgca ggagtggcaa cagcttgcca
     9841 acaaggaatt gagccgacgg gagaaaactg tcgactcgct ggttcagcaa accgcggaag
     9901 ggatcgccat caagccgctg tataccgaag ccgatctcga taatctggag gtgacgggta
     9961 cccttcctgg tttgccaccc tacgttcgtg gcccgcgtgc cactatgtat accgcccaac
    10021 cgtggaccat ccgtcagtat gctggtttct cgacagcaaa agagtccaac gctttttatc
    10081 gccgtaacct ggccgcccac cgaggctacg actccgataa cccacgcgtg gcgggcgacg
    10141 tcggcaaagc gggcgtcgct atcgacaccg tggaagatat gaaagtcctg ttcgaccaga
    10201 tcccgctgga taaaatgtcg gtttctatga ccatgaatgg cgcagtgcta ccagtactgg
    10261 cgttttatat cgtcgccgca gaagagcaag gtgttacatc cgataaactg accggcacca
    10321 ttcagaacga tatcctcaaa gagttcctct gccgcaatac ctatatttac ccgccaaaac
    10381 cgtcaatgcg tattatcgca gacatcatcg cctggtgttc cggcaacatg ccgcgattta
    10441 ataccatcag tatcagcagt taccacatgg gtgaagcggg tgccaactgc gtgcagcagg
    10501 tagcatttac gctcgccaat aggattgagt acatcaaagc agcaatctcc gccgggctga
    10561 aaattgatgg cttcgtccct cgcctgtcgt ttttcttcgg cattggcatg gatctgttta
    10621 tgaacgccgc catgttgcgt gcggcacgtt atttatggag cgaagcggtc agtggatttg
    10681 gcgcacagga tccgtataac aacgttatcc gcaccaccat tgatcattgt gcacatccta
    10741 tgtgtcggga ttatctgcat cgctatctgg aaaatgcgtc cggcggacat attcaccacg
    10801 atcttagcca cgtcttcgac ttacaccgta atttaattgc aaccggatcg atgctgggtt
    10861 aatggtttgt cccgtcacga tcttcagcaa tatgctgaag catcgtggcg gtataacgat
    10921 gatttctgat tgaataaaga tattcctgca tatacatcgg cctgtgcggc gcatcaaaat
    10981 atttcagcgt tgccggattc accagtcgcc aggcaaaatg cgggatcacc gtcagaaaat
    11041 gtccccgctc cacgtcgctg attttggcca taaaactata gggacgataa ataatggttg
    11101 gattgatccc acaaggacgc atattcgcat caatcattgc ttcgaagtta gcccggtttt
    11161 gaaagcgcat ttgtagccag ggcagctcct gaagcagctc ttgctcttgt cgctcttcat
    11221 aacggcgaga aacgagaaag cccaatcgca gcggcggtag ttcactgata gtcaggtttt
    11281 ccagttcctg aactcgtgca gatacatgct gtggggagat aataaaatct aactgacggt
    11341 caaaaagatt atcgataacg ccatttgcgc tgaactcaac tggctgggcg gtcacgcctt
    11401 cgtacttatc tccgagacta atcaactgat caaaaataat ggtcggataa gtattatcta
    11461 tgcctatcac aatgtctcgt gaacgacgtc ctgagttatg gatttcatta tcaatcgcag
    11521 aaagtcgttg ataaactggg aacagttttt gatacagttc ctgtccggct ttattgaggc
    11581 tgatattatt atctttgcgg gtaaatagcg tatagccaat ttgttcttcc agcgcggcaa
    11641 tgcttttgcc aaatcgcgaa gccgtcatat ggattttttc tgccgcccgg gcaatattat
    11701 tggtttgcgc taataaaatg aaattgcgca tctttttcga aataaaaatg tccatccccc
    11761 ctctcccgca tccttgtccg cttaaccgtt tcagtcagcc tatccttgat gaaaccgcga
    11821 gcaaagatag gtgattacgt catggtttta cagaaaaagg agacaatatc gggtaaagac
    11881 attagcccga cgaatacgtc gggctacaag tattaatgtg cagcaggtgt tttagcgggg
    11941 ggttgatcca caggttctaa ctggaagact acatcgacct gatcatcaaa ctgaatagcg
    12001 gcctgctcgt aagtttcctg tgcggacacc ggtgcagcat cagctttcat catccgcacc
    12061 attgggctgg gctgatagtt ggaaacatga taacgcacgc tatataccgg ccccagtttg
    12121 cgattaaagc cattcgccag ttcctgcgcc tgatgaatcg cgttatcaat cgctgcctta
    12181 cgcgctttgt ctttataggc atccggctgc gccacgccca gcgacacaga acgaatttcg
    12241 ttaagacccg cctttagcgc gccgtccagc aaggaattca gtttgtctaa ctgacggagc
    12301 gtgacttcca ccgttctcac tgcgcggtag cctttcagga tacttttacc atcctgataa
    12361 tcataatctg gctgagtgcg taagttcgct gagctgatat cttttttcgc gatctgattg
    12421 agctcaagga aggaaatgta ttgtgcgacg cgctcatctg cctgtttctt ggcagtagcg
    12481 gcatccttcg cggccacgtt aacttcaatc gcaagagtgg caatgtctgg caccgcatcc
    12541 acgcttgccg taccggaggt gacaatatgc ggtccatccg gcaattcgtt agcctgcgct
    12601 gccatcccgc taatacccat taatgccgcc agggcgataa ctttgaactt cacgttagtc
    12661 tctccgtgtt aattgacttg cccaaaattc aggacctctg gaggcaagct tagcgcctct
    12721 gttttatttt tccatcagat agcgcctaac tgaacaaggc ttgtgcatga gcaataccgt
    12781 ctctcgccag ctgcaaggca ataaaccaca taacacatcc cactaccaga ttgataatgc
    12841 gctgtgattt tgccgtgcgc aggcgcggtg ccagccaggc tgcgagaata gccagaccaa
    12901 agaaccacag gaaagaggcg ctaattgttc cgagcgcaaa ccaacgtttt ggttccacat
    12961 caagttgccc gccaaggctg cccagcacaa caaaagtatc caggtaaaca tgcggattca
    13021 gccaggtcac tgccaacatg gtggcgataa ttttccatct gccttgtttc aggacttcgg
    13081 cactggctaa ctcaatgtta ctgctcattg ctgttttaaa agcgccaaaa ccataccaca
    13141 gcaagaaggc tacgccgccc caggtgacca gcgccagcaa ccacggcgac tgcatcaata
    13201 acgcgctgcc accaaaaatc cccgcacaaa tcaggaccaa atcgctgata gcacaaagta
    13261 aggcaatcat aatgtggtac tgacgacgaa tgccctgatt catcacaaaa gcattttgtg
    13321 gaccgagcgg taggatcata gccgccccaa gtgcaagacc ttgaaagtaa taagaaaaca
    13381 cgttggatat tccgaattaa gtatctgatg cagagactat atcgcgggat tgttattagc
    13441 ggaaattgat aatattaatt ggcaatcaga aaaactaata agagagaggc cagagagtat
    13501 tatctggcct cagcgttgat tacgcagctt tgtcttcttt cacccgctta aagttcacat
    13561 ccatttgcgg gtacgggaag ctgataccgg cggcatcaaa ttcacgttta atacgctcca
    13621 gcacatccca gtacacgttt tgcagatcgc cgctgttgct ccagacgcgg accacgaaat
    13681 taatcgacga tgcgccaagt tcgttcagac gcacagtcat ttcgcgatct ttcaaaatgc
    13741 gatcttcaga ctggataata tcggtcagga tctgcttaac ctgatcgata tcggaatcat
    13801 acgccacgcc aataataaat tcgttacggc gagctggctc gcgggagaag ttaataatat
    13861 tcccggcaat aattttaccg ttcggaataa cgataatttt accgtctgca gtacgcatgg
    13921 tggtggagaa aatctgcaca ctcagcacag taccggctac gccgccgagg tcaacatatt
    13981 ctccggcacg gaacgggcgg aacatgacaa gtaacacgcc agcggccagg ttagacagtg
    14041 acccctgcaa agccagacca acagctaaac ctgcggcacc gagtacagca atgactgacg
    14101 ctgtttgtac gcccacgcgt cccagtgctg cgattagcgt aaaggcgata attccgtaac
    14161 ggactaatgc agaaagaaaa tcagcaacag tggcatcgat tttacgggag atcatcaggc
    14221 gattcaccgc gttggaaatc atccgcgcga taatcaaacc aacgatgatg atcgcgagtg
    14281 ccgccacgat gtttactgca taacttagca gcagcgcctg gttagccacc agccagcttc
    14341 ccgcgccgtt tatgctatcg acaacattca aatcttccat tcaatattcc ttttcaactg
    14401 actcaaaatg gagaaataca ctccgccgcc ttatgacggg cagtctgaca agggtaaaca
    14461 ataaagcata attttgccaa atagatcaca gatttctcat ctgtatcacc ttgcttttgg
    14521 cgaaaaaaag acccgcagag cgggccttga gataagcaga aaggaatatc ttacagaacg
    14581 tcgatcgcgt tcagttcctg gaatgctttt tccagacgag cgatcatcga agtctgaccg
    14641 gcacgcagcc atacgcgcgg atcgtagtat ttcttgttcg gctgatcttc gcctttcggg
    14701 ttgcccagct ggccttgcag gtaagcttcg ttttctttgt agtagttcag aacaccttcc
    14761 caggttgccc attgggtatc ggtatcgatg ttcattttca ctacgccgta gcttacggag
    14821 tctttgattt cctgagcagt agaaccggaa ccaccgtgga agacgaagtt caggctgttg
    14881 tgcggcaggt tgtgtttctt agaaacatat tcctgagagt cgcgcaggat ggtcggagtc
    14941 agaaccacgt tacccggctt gtaaacaccg tgtacgttac cgaaggacgc agcgatggtg
    15001 aaacgcgggc tgattttgct cagctcggtg tatgcgtaat caacgtcttc cggctgggtg
    15061 tacagtgcag aagcgtccat gtggctgttg tccacgccgt cttcttcacc accggtgcaa
    15121 cccagttcga tttccagagt catgccaatt ttggacatgc gctccaggta tttagagcag
    15181 atttcaatgt tctcttgcag agattcttca gacaggtcaa tcatgtgaga agagaacagc
    15241 ggcttaccgg tagcagcgaa gtgtttttca cccgcgtcca gcagaccgtc gatccacggc
    15301 agcagttttt tcgcgcagtg gtcagtatgc aggatcaccg gaacaccgta gtgttcagcc
    15361 atctggtgaa cgtgatgcgc accagagatc gcacccagga tagcagcgcc ctgcggaacg
    15421 tcagatttca cgcctttacc agcgataaaa gaagcaccac cgttggagaa ctgaacgata
    15481 accggcgctt taactttagc tgcggtttcc agtacggcgt tgatggagtc agtaccgacg
    15541 cagtttactg ctggcagtgc gaagttgttt tcttttgcta cctggaaaac tttctgtacg
    15601 tcatcaccag tgattacgcc aggttttacg aaatcaaaaa tcttagacat gtctcttgtc
    15661 ctgtatcgtc gggccttata ctcgtcatac ttcaagttgc atgtgctgcg tctacgttcg
    15721 ctcaccccaa tcacttactt atgtaagctc ctggggattc actctcttgt cgccttcctg
    15781 caactcgaat tatttagagt atgaaaaagt gcgtgctcta aaagcgcgct gaaacaaggg
    15841 caggtttccc tgccctgtga ttttttactt cttagcgcgc tcttcgagca tcgctactgc
    15901 aggcagtact ttaccttcca cgaattcgag gaatgcgccg ccgccagtgg agatgtagga
    15961 gattttgtca gcaatgccga acaggtcgat tgctgccaga gtgtcgccgc cgccagcgat
    16021 ggagaacgct tcgctgtctg cgatagcgtt agccacgatt tcagtacctt tgcggaagtt
    16081 cgggaattcg aacacgccaa ccggaccgtt ccacagaatg gttttcgcat tcttcaggat
    16141 ttcagccagt tcctgagcgg aagcatcacc gatatccagg atctgctcgt cagctttcac
    16201 atcgttaaca gatttcaggg tagccggtgc agtttcagag aactcggttg ctacgcgaac
    16261 atcagacgga accgggatgt tgcaggtggt cagcagacgt ttagcttcgt caaccaggtc
    16321 agcttcgtac agggatttac ccacatcgtg gccttgtgcc gcgataaagg tgttagcaat
    16381 accaccacca acgatcagct ggtcagcgat tttagacagg gagtccagaa cggtcagttt
    16441 ggtagatact ttagaaccac caacgatagc caccatcgga cgagcaggtt ctttcagtgc
    16501 tttacccagc gcgtccagtt cagctgccag cagcgggcct gcgcacgcaa cgtcagcgaa
    16561 tttaccgata ccgtgagtag aagcctgcgc gcggtgagca gtaccgaatg cgtccattac
    16621 gaacacgtca cacagtgcag cgtatttttt ggacagagtt tcgtcgtctt tcttctcgcc
    16681 tttgttgaag cgaacgtttt ccagaacaac cagttcacct tcagcaacgt caacgccgtc
    16741 gaggtaatct ttaaccagac gaaccgggtt agacagtttg tctttcaggt agttaacaac
    16801 cggcagcaga gagaattctt cgttgtactc gccttcggta ggacgaccca ggtgggaagt
    16861 taccatcact tttgcgcctt gtttcagggc cagttcgatg gtcggcagag aagcacggat
    16921 acgcgcgtcg ctggttactt tcccgtcttt tactggtacg ttcagatccg cacggataaa
    16981 tacacgtttc ccagcaagat ccagatcggt catcttaatt acagacatgg tgaatcctct
    17041 cgttgattct aaaagttttg cagacgctgc ttgcgtctta cctgaaagca acagtagcca
    17101 tagctaacgt cgtgtcgagc attcggttag caaagcccca ttcgttatcg caccagacca
    17161 acgttttgat caggtgtgcg ccactgaccc gggtttgagt gccatcgaca atggcactgt
    17221 gcggatcgtg gttaaaatct acagagacca acggcaattc cgtatagtca actataccat
    17281 gaaatgcacc ttgtgctgct ttttgcagca acaggttgac ttcattggct tttacaggtt
    17341 tcttcactgt cacgcttaaa tcgattgccg tcacatttat ggttggcaca cgtaccgcaa
    17401 tcgcctcaaa gcgatcgtta aattgcggaa aaaatcgtgt gataccggcg gccagtttag
    17461 tatcgaccgg aatgatcgac tgactggctg cccgagtgcg acgcaggtct ggatggtatg
    17521 catcaataac ctgttgatcg tgcatggcgg agtgaattgt ggtcacagtg ccggactcaa
    17581 taccgtacgc atcatctaac aatttgatga cgggaattat gcaattcgtg gtacaggaag
    17641 cgttagaaac gatgcggtgt tccgcacgaa gttgatcctg attgacgcca taaacaacgg
    17701 tcgcgtcgag atcgttactg ccaggatgtg aaaagagcac ttttttagcc ccggcggcaa
    17761 tgtgtgcttc accatgctcg cgggagccat atacgccggt gcagtcgagg actacatcaa
    17821 cgccaagttc acgccagggg agcgattgca gtgaacgttc atgcaataca cggatggcgt
    17881 catcaccaac aaaaagttga tcgcgttcct gtcgtacttc ccatgcaaaa cggccatggc
    17941 tggtgtcata tttcaacaaa tgcgccatac ccgcagcatc cgccagttcg ttgattgcca
    18001 ccacggtaat ttccgcacgg cgtccggatt catacaaagc acgaaccaca ttacgcccga
    18061 tgcgaccgaa gccatttatc gctacgcgta cggtcataga tctcctgcaa ggttttccct
    18121 gagcaaattt gccagacaga gtaatccagc aaatcgttcg gggaaacctt acctgtcgca
    18181 aactgcgact gattggttaa ttgtcgaaca tttaatcgac tgaaacgctt cagctaggat
    18241 aagcgaaacg tggaataaaa ggaatgattg tccagtcaaa gaagacaatt atctgactta
    18301 cgtcacgttt tcagccccgc ccctgcggaa tttattccac tttgcagaat cacgaataag
    18361 cgtagcaaaa tcttttcgtt gtgccgtcac aaaccctgac ttatatcaga atatgactga
    18421 tgcagcgtcg atattatccg caatacgtcc gagtttcgga gtactccaat gatgacattg
    18481 ccctacccat attcttcctc cgcacgcttt tacgcgctgc gcctgctccc cgggcaggaa
    18541 gttctctccc aattacgcgc cttcgcgcag caacaacaac ttcacgccgc gtggattgca
    18601 gggtgtaccg gcagtctgac tgacgtcgcc ttacgctatg ccgggcaaga aaacactgcc
    18661 ctgctaagcg gtaagttcga agtcatcgca ttgaacggca cgctggaaca gagtggtgaa
    18721 catcttcatc tctgcgtttc cgatccacac ggcacgatgc ttggcggtca tatgatgccc
    18781 ggctgtaccg tgcggaccac gcttgaactg gtgatcggct gcctggagga actggcgttt
    18841 agccgccagc tttgcgcgct ttctggttac gacgagttgc atatttcccc cgttaaataa
    18901 ctattttcag agaggttttc ttatggcgcg tcgccacttt tccagtcagg cactggtgtt
    18961 gatcgttatc tctatcgcca tcaatatgat cggcggacaa ctggccagca tggttaaatt
    19021 acctattttt ctcgactcca tcggcacact tattagcgcg gtactgcttg gtccggtgat
    19081 cgggatgctg accggtttac tgaccaattt actctgggga ttgctgaccg acccgatcgc
    19141 cgccgcattt gcgcccgtcg cgatggtcat cggcctggtc gccggttggc tggcgcgagc
    19201 gggttggttt cgcaccctgc caaaagtcgt tgttagcggc gtgattatta cgcttgcggt
    19261 aacggtcgtc gctgtaccgc tgcgtactgc gctgttcggc ggcgtgacag gaagcggagc
    19321 cgatttgttc gttgcctgga tgcactcgat ggggcaaaac ctggtggagt cggtggcgat
    19381 tacggtgata ggcgcgaacc tggtcgataa aattctcaca gcggtgattg tctggctttt
    19441 actgcgtcag ctgccgattc gcaccacgcg ccattttccg gcaatggctg ccgtgcgcta
    19501 aatgcatccg tttacatcct taacgctgtg ggcgctggcg gcctgcacca cgcttatcct
    19561 gcccacacag acaatattgc cagtctatag cgcggcgacc tttttctgcc ttattgcgct
    19621 aaaggccacg cggcggcggg caaaatatgt cgtctggctg atgttctcgc tgggggctgg
    19681 attgtggctg gtgcacggtg gctggctgac agaatggcta agcggcacgc cacgctctcc
    19741 agaacggtgg gcgcatgcta ttacgctctg gttgcggatt ctggcgattg tttcaacctc
    19801 acagttgtgg atgcagtacg tccccgtgca acgttttatc cgcgctttgt ttgcttctcg
    19861 cctgccgccg ggcgtcgctt atctgtttgc cgggccgtta ttggtcgttg agcagttaaa
    19921 acggcaattg gcgattattc atgaagcgca gcgcgcccgc ggcgtgccgc tggatgaggg
    19981 ctggtatcag cgtttacgtg cgatgcccgc gttaattatt ccgctaacac acaatgcgct
    20041 aaacgatctt gctgtccgtg gtgcggcgct ggatatgcgg gcatttcgaa tcaacaatag
    20101 gcgcactacg ttatgggcac cagccgatag caccctacaa cgcgtagcac gttacaccat
    20161 gatattgcta atgctggctg aattcggagc gtggatatgg ttacgttaga acagtttcgc
    20221 tattgcccga cacactcaac gcatcctccg ttctgttatg actttcacta tgtcaaaccg
    20281 gggatggtcg ccatctttgg tgacaacggc agtggcaaaa gtacgctggc gcagctgatg
    20341 gcgggctggt atccggactt tctaccagga gagataaccg gcacggggac gcttctcggt
    20401 acgcccattg ggcgtctccc gcttaatgaa caatccgcca ccatccagct cgtccagcaa
    20461 tcgccctatt tgcagctctc cggctgtacc tttagcgtgg aagaagaagt tgcctttggg
    20521 ccagaaaatc tgtgtcttgc tgaaaaagag attatggcgc ggattgacgc cgccctggcc
    20581 ctgaccgagt gccagccgtt gcgccatcgt cacccggcaa cactctccgg tggagagaca
    20641 cagcgtgtgg tcatcgcctg cgccatcgcg atgcagccaa agctcttaat tcttgatgaa
    20701 gcctttagcc gcctgacgcc gcaggcccgc gaaatgctgt tgcagcgctt acaacactgg
    20761 gcgcttgaac gtggttcgct gattattctt tttgaacgtc atcacacacc tttcctgaac
    20821 cactgccagc aagcgtggca attgcaaaac ggagcgctcc agccactatg ttaacgttaa
    20881 atcagatatc ttatcgctgg cctggcgcgg caacggattg tctttgcgac atttctctgc
    20941 aactcaaaca aggtgaatgg ctggcgctga ccggcgataa tggcgcggga aaatcgacgc
    21001 tgttacgagt aatggcaggt cttcttacgc ccacagccgg tacggttatg ctgcaacaac
    21061 aagcgatgaa aaacctgaaa aaccgccagc gcgcggcaaa gattggcgta ctgtttcagg
    21121 aagcggaaaa ccagcttttt cacagcaccg ttgctgacga gatcgccttt ggtttgaaac
    21181 tgcaaaaatg cccggcggat gaaatcactc aacgtaccaa tgctgccctg caatgctgtc
    21241 agttagcaga cactgccagc gcgcatccgt tagatctgca tagtgcgcag cgacggatgg
    21301 tcgccgtcgc cagcctggaa gcactctctc cacccctatt gctactggac gagccgagtc
    21361 gggattttga cgaaaattgg ttgagcgtgt ttgaaagctg gctggagaag tgccgccagc
    21421 gaggcacgag cgtcgtagca atcagtcatg acgccgcatt tacccggcgt catttctctc
    21481 gtatggtgcg actggaagat ggtgtaatca ggaacgttaa tccatcagac gatattcacc
    21541 catagcggtc atcattaagg tcaaatttgc aggcagactc tcttccagca cctggcgaac
    21601 attcggcccg tcggtacggt cataaaaggc ttctgcgtct gccagcgata accctcccgc
    21661 cagcttgcgc cccaccagac gctcccgcaa cgcagatgcg ggagctttga taaagatcga
    21721 gaaatcacaa aattgcgcaa gctggcacca cttctcgtcg tccaacaaca accagtttcc
    21781 ttcgacgatg acgagtggtg cggtaacgtg caacacatct tcaacaggat catgcttttg
    21841 tcgatcgtac tgcggccacg tacaatcccc ttccacaacc cggcacagat tttccgccag
    21901 tttcgcaacg ttgaatgtct ctggtgcgcc tttgaagggt cgcaattgat gcacatccag
    21961 ccagctattg taatggtgaa aaccatccat cggcagcgtc tgaatagcgg gcagttccgg
    22021 gtcttgttgc gcgagatatt cccagaaggt ggttagggta gatttacccg tccccggcgg
    22081 agcgcaaaga aaaaccaccg tacgccgctg cggatttact gtctgtaagg ccgccagcat
    22141 acgcagcaat ggtttatgta cattttcaat ttcatcatca gagtactgcg cctgtacctt
    22201 tagtccattc accgtcagtt cgattttcac ggcgttaatt cctttaaggt ggtcgttact
    22261 gccagcgtca gcgccgtttt gaccatcccg ctaaaacgcg cctctgacca gacggcgaaa
    22321 tcggcgaact tcatcgcttt caacgcgtca tagagcgtcg cattgcgggt tatgctattg
    22381 acgttgctga taaaatccca ggttatgtca tcggcaaatc tgggcgtttc atcctgttcg
    22441 ttcaggtaat gccaaaactg ggaaaaatgc gtgatatcgg cgtacagcca tttatccatt
    22501 ttccccagcg cataaatcaa acgcagcgca acatcaatat cgtcaagcgg gccgctttgg
    22561 gcgagtaacg gcttcactgc atattcgacg atctcttcat cgttatcgac aaacagcgac
    22621 ggcagaaact ggcgtatccc ctggagcaaa atagaatgcg cagttttcat aaatgaaaat
    22681 aaattgtcct gtgcattcag ttgctcaagc acatcatctt ctgtcagcgt cgccattggt
    22741 cttccgatag atcaccatca atgcagggag tctattatat tacacgtttg gtccgcgccg
    22801 ccgatcagta atgtctgcgt ccgcacccca ttcgccgtct gttggatacc gttcaccaac
    22861 tcgccgcccg tcacgcccgt agcgctaaag aggatatcgt tccccctcgc cgataaccac
    22921 tcgcccacga aaagcaacgt cgtttaatgc ctggcgcatc gcggtgaccg caaggccatc
    22981 aattttattt ttgtcgccac agccggtttg cggccaggca gccaacgctg cctgttccgt
    23041 aacgcgaaat aatggccacg ccagggacat cattgcgcgg cctccccaat atcaacgcca
    23101 aattgtgcca gcagatattt ctcggcctgt tcattccaga tgccgtgcgt tttctcaacc
    23161 agccgcgcca gctctttaaa cagcggatcg gttttgcctt tttcggcaaa atcggcaatg
    23221 gcggtcagcg gcatggtcac gccgttataa atgagttttt taccgccagg gatatccggc
    23281 agattgagca cggtttctgg caccgcatcc aggccaccga tatgggtcac cataaacgac
    23341 ggctgtaact gccccgtggc gctaagggca atcgcctctt tcatgtcgtc cgtggaaccg
    23401 ccggacgtgc cgacaacgtg cgtactgttg taatggacgt tgtagaaatt aaacggcact
    23461 ttgaagtttt tatccgtcgg accggcaaag aagttcaggc agccatcttc agccagtaat
    23521 tcatccgcca tctcgacgac tgaaggcacc gctgcataga caaatacgtc atcaaatccg
    23581 gcatcatccg tcagcgcccg cagcgtctgg accggatcgc tcatcccttt ggtattcata
    23641 tacaccagct caatgccttt actggccgcc agttccaccg gcagcagctt ttgtacctgc
    23701 gtcaggcgtt tgtcgtcgat atcgactacc accacccgcg ccggttgtat gccgccgtta
    23761 atggcgtaat caatagcgcc aattcccatc gggcctgcac acgccagtag cgcaatattg
    23821 ccaccaggtt tgacgcccat gcgatgctca taaacatatt gcgtggtgtg ataattggca
    23881 tgataagcgc caataatgca gcacatcggt tccgccagcg aggccgcagc aaaataagag
    23941 ccgtgatacg gtaatacgca gcccaggtta atggcgattt cgggaataat catataggtg
    24001 gcgttaccgc caaaatattc gtagctgtag cccgctgaat atccgcctgg taaccccatc
    24061 gccggttgca atacaaaacg ctggcctttt ttatatttgc cagtgagatt tttacccact
    24121 tcgacaataa ccccggcaca ttcatgcccg gtaatgaccg gatgattttc taaatcgtcg
    24181 gggacgcgtt tatgttcact accgagtaac gccgctttcc aggtggataa acaaacgctg
    24241 tcagaaatta cattcactaa cagttcatta tcggtaattt ctggcagctc aaattcgcgc
    24301 agacggacat cccgcttgcc ataaatagca gcaactttgg ttttcatttt tacctctgta
    24361 atcagtgttt atgttcagcg gttaattgat taaataaagt gtcgagtttt ggatcgccaa
    24421 tatagttatt aatcaatatc agtggtttat ccgtcacacg tttcacgcgc ccttccagac
    24481 tggcgtgagt aacgacgata tccgcatccg caggcacgtt ttctatggcg taatgtttta
    24541 cttctattgc cagccccgct ttttccaggc gtttacggaa ggtggttgcg cccatcgcac
    24601 tggagcccat ccctgcatcg caaacaaagg caatacgctt aacacggctt aacgagaatg
    24661 caccttcttg tttcatcgcc ttaaccgcat tggctgactg agcaaactca tcttcgctct
    24721 tcgtttccac cgttttttcc atcttcagta tcagcgaagt aatagcaaag gacaccaggg
    24781 tacctaccgt aacaccggca attgtcgcca ggaacgagcc tttcggcgtc agtgccaggt
    24841 aagcaaagat agaccccgga cttgggccag ccaccagacc accgtccagt aagttaaaca
    24901 tccaggtgcc agacataccg cccgcaatca tggcaataat ggtcagcggc ttcatcagca
    24961 catacgggaa atacagttcg tggatcccac cgaggaagtg aataatcatc gccccgggcg
    25021 cagaacgttt actcatccct ttaccaaaca aggtaaacgc cagcagcagc cccagaccag
    25081 gacctggatt ggaggccacc atgaagaaga tggatttacc gttaaccgaa gcctgttgca
    25141 ttcccagcgg ataatagacg ccctgatcga tcgcattatt aaggaaaagc actttcgccg
    25201 gttcattgat gactgacagc aaaggtagat aacccgcatg taccagcgcc tcaatacact
    25261 ctttgacgaa agtattagca attaacaccg ccgggccgat aacttcaaaa cccagcagac
    25321 aaaggagcat ccccgcgata cctaatgaga agttattgat aaccatctca aaaccagcag
    25381 gtatgcgttt ttccagcgac ttgtcgatat atttaatcac cagaccaccg agcggcccca
    25441 taatcatcga gccaaggaac atcgggatct ctgcgccgac gatcacacca atagtaccaa
    25501 ttccgcccat gaccgcgccg cgtttaccgc cgaccagatg accaccggta gaaccaatca
    25561 tcacgggcaa taaataggta atcatcggac caacaatttt ggcgaaatgt tcattcggca
    25621 gccaaccggt gggaataaat aatgccgtta taaatcccca ggcaataaaa gcaccgatat
    25681 tggggatgac cattgcagtc aaaaatcccc caaaagcctg gacctttgca cgagcagact
    25741 tgttttccat aatattatcc tgtagaggag agagtcatta tccgcggctg ataatgtccg
    25801 ctaattgttt ttctgatgat gcggttaaga gttgttcgag tatttcttct tcgcacagta
    25861 attcacttaa cgcctgaata gcaccaatgt gcgaatcggc atcggcagca gaaagtccga
    25921 tgagtaattt aatcggttcg tcattccccg gaaaataaac gccttgttta agtaacgtca
    25981 aagacatccc ggttttaagc gctccacatt ccggtcgcgc atgaggcatt gccacgcctg
    26041 gtgcgagaat ataataagga ccattgctga tggtggaatc tttaatagcc tgaatgtaat
    26101 tctcgctgat atagtttttt gctaacagtg atgccatcga gaaattgata gcttcctgcc
    26161 agtcttttgt tgaatgtata actgagattg ataattctgg aaaataatca ataagccgca
    26221 taaattatcc ttatttattt ttagggtacc g
//
LOCUS       DADSZD010000056        25930 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.056, whole genome shotgun sequence.
ACCESSION   DADSZD010000056 DADSZD010000000
VERSION     DADSZD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.056"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..356)
                     /locus_tag="J1Z28_002041"
     CDS             complement(<1..356)
                     /locus_tag="J1Z28_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA7716876.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTRE"
     gene            complement(427..2541)
                     /gene="fusA"
                     /locus_tag="J1Z28_002042"
     CDS             complement(427..2541)
                     /gene="fusA"
                     /locus_tag="J1Z28_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBA7716877.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2638..3108)
                     /gene="rpsG"
                     /locus_tag="J1Z28_002043"
     CDS             complement(2638..3108)
                     /gene="rpsG"
                     /locus_tag="J1Z28_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006714698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBA7716878.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3205..3579)
                     /gene="rpsL"
                     /locus_tag="J1Z28_002044"
     CDS             complement(3205..3579)
                     /gene="rpsL"
                     /locus_tag="J1Z28_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003852912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBA7716879.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3705..3992)
                     /gene="tusB"
                     /locus_tag="J1Z28_002045"
     CDS             complement(3705..3992)
                     /gene="tusB"
                     /locus_tag="J1Z28_002045"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBA7716880.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3999..4358)
                     /gene="tusC"
                     /locus_tag="J1Z28_002046"
     CDS             complement(3999..4358)
                     /gene="tusC"
                     /locus_tag="J1Z28_002046"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBA7716881.1"
                     /translation="MKRIAFVFSTVPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4358..4744)
                     /gene="tusD"
                     /locus_tag="J1Z28_002047"
     CDS             complement(4358..4744)
                     /gene="tusD"
                     /locus_tag="J1Z28_002047"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBA7716882.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRSWQQLNMQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQTGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4744..5466)
                     /locus_tag="J1Z28_002048"
     CDS             complement(4744..5466)
                     /locus_tag="J1Z28_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7716883.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5633..6445)
                     /gene="fkpA"
                     /locus_tag="J1Z28_002049"
     CDS             complement(5633..6445)
                     /gene="fkpA"
                     /locus_tag="J1Z28_002049"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003827768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA7716884.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEANAKAADSAKK"
     gene            6666..6884
                     /locus_tag="J1Z28_002050"
     CDS             6666..6884
                     /locus_tag="J1Z28_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBA7716885.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6934..7524)
                     /gene="slyD"
                     /locus_tag="J1Z28_002051"
     CDS             complement(6934..7524)
                     /gene="slyD"
                     /locus_tag="J1Z28_002051"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA7716886.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7619..7819)
                     /locus_tag="J1Z28_002052"
     CDS             complement(7619..7819)
                     /locus_tag="J1Z28_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBA7716887.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7829..9634)
                     /gene="kefB"
                     /locus_tag="J1Z28_002053"
     CDS             complement(7829..9634)
                     /gene="kefB"
                     /locus_tag="J1Z28_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBA7716888.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9634..10185)
                     /gene="kefG"
                     /locus_tag="J1Z28_002054"
     CDS             complement(9634..10185)
                     /gene="kefG"
                     /locus_tag="J1Z28_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBA7716889.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10316..12229
                     /locus_tag="J1Z28_002055"
     CDS             10316..12229
                     /locus_tag="J1Z28_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7716890.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12229..13251
                     /locus_tag="J1Z28_002056"
     CDS             12229..13251
                     /locus_tag="J1Z28_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBA7716891.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PHWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13245..13463
                     /locus_tag="J1Z28_002057"
     CDS             13245..13463
                     /locus_tag="J1Z28_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBA7716892.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13517..14386
                     /locus_tag="J1Z28_002058"
     CDS             13517..14386
                     /locus_tag="J1Z28_002058"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBA7716893.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14441..14845)
                     /locus_tag="J1Z28_002059"
     CDS             complement(14441..14845)
                     /locus_tag="J1Z28_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBA7716894.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15147..15779
                     /gene="crp"
                     /locus_tag="J1Z28_002060"
     CDS             15147..15779
                     /gene="crp"
                     /locus_tag="J1Z28_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBA7716895.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15830..17920
                     /locus_tag="J1Z28_002061"
     CDS             15830..17920
                     /locus_tag="J1Z28_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716896.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLILGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17987..19207)
                     /gene="argD/dapC"
                     /locus_tag="J1Z28_002062"
     CDS             complement(17987..19207)
                     /gene="argD/dapC"
                     /locus_tag="J1Z28_002062"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBA7716897.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPVSAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGAMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19293..19856)
                     /gene="pabA"
                     /locus_tag="J1Z28_002063"
     CDS             complement(19293..19856)
                     /gene="pabA"
                     /locus_tag="J1Z28_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBA7716898.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19888..20490)
                     /locus_tag="J1Z28_002064"
     CDS             complement(19888..20490)
                     /locus_tag="J1Z28_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBA7716899.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20480..20647)
                     /locus_tag="J1Z28_002065"
     CDS             complement(20480..20647)
                     /locus_tag="J1Z28_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBA7716900.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20752..21324)
                     /gene="ppiA"
                     /locus_tag="J1Z28_002066"
                     /gene_synonym="rotA"
     CDS             complement(20752..21324)
                     /gene="ppiA"
                     /locus_tag="J1Z28_002066"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBA7716901.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21595..22776
                     /gene="tsgA"
                     /locus_tag="J1Z28_002067"
     CDS             21595..22776
                     /gene="tsgA"
                     /locus_tag="J1Z28_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBA7716902.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23038..25581
                     /locus_tag="J1Z28_002068"
     CDS             23038..25581
                     /locus_tag="J1Z28_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit"
                     /protein_id="HBA7716903.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25578..25904
                     /gene="nirD"
                     /locus_tag="J1Z28_002069"
     CDS             25578..25904
                     /gene="nirD"
                     /locus_tag="J1Z28_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBA7716904.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
BASE COUNT         6329 a         7009 c         6502 g         6090 t
ORIGIN      
        1 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccgtcc
       61 atctgagcag caccggtgat catgttttta acatagtcgg cgtgccccgg gcagtctacg
      121 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      181 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cacgagcagc accgccgtag
      241 gttttagcca gtacggtggt gattgcagcg gtcagagtag ttttaccgtg gtcaacgtgg
      301 ccgatagtac caacgttaac gtgcggtttt gtacgttcaa atttttcttt agacacggct
      361 atattcctta ctatagtgct ctccccttca ggagagagca cgggactttg gtattaaccc
      421 ttaggcttat ttaccacggg cttcaattac ggcctgagca acgttactcg gcgcttcatc
      481 atacttcagg aattccatag tgtatgatgc gcgacctttg gtcagagaac gcagctgagt
      541 tgcatatccg aacatttcag acagcggtac ttcagcgtgg atcttaacgc cagtaacttc
      601 agattcctga cctttgagca taccacgacg acggctcaag tcaccgataa cgtcaccggt
      661 gttctcttca ggagtttcta cttcaacctt catgatcggc tcaagcagaa ctggtttcgc
      721 tttcttaaag ccttctttaa aggcgataga agcagccagt ttaaacgcca gttcagagga
      781 gtcaacgtca tggtaagaac cgaagtgcag acgaataccc atgtctacta ccgggtagcc
      841 tgccagcgga cctgctttca gctgttcctg gataccttta tcaacggccg ggatgtattc
      901 gccagggatt acaccacctt taatgtcgtt gatgaactcg tagcctttcg ggtttgaacc
      961 cggctccagc gggtacatgt cgataacaac atgaccatac tgaccacgac caccagactg
     1021 tttcgcgtgt ttaccttcaa catcggtaac tttctggcgg atagtttcac ggtaagcaac
     1081 ctgcggttta cctacgttcg cttcaacgtt gaattcacgc ttcatacggt caacgatgat
     1141 gtcgaggtgc agttcgccca tacccgcgat gatggtctgg ttagattctt cgtcagtcca
     1201 tacacggaaa gacgggtctt ctttagccag acggcccaga gccagaccca ttttttcctg
     1261 gtcagctttg gttttcggtt caactgcgat ggagattacc ggctcaggga attccatacg
     1321 ttccagaatg atcggcgcat ccgggtcaca cagggtgtca ccagtggtta cgtctttcag
     1381 accgattgca gctgcgatgt cgcccgcgcg aacttctttg atctcttcac gtttgttagc
     1441 gtgcatctga acgatacgac cgaaacgctc acgtgcagct ttcacggagt tcagtacggt
     1501 atcaccagag ttaaccacac cggagtacac acggaagaag gtcaggttac caacaaacgg
     1561 gtcggtagcg attttgaacg ccagtgcaga gaacggctcg tcatcacttg cgtgacgctc
     1621 agccggagtg tctttaccgt cgtccaggat accgttgatc gcaggtacgt caaccgggga
     1681 tggcaggtaa tcaattaccg catccagcat cgcctgaaca cctttgttct tgaacgcaga
     1741 accacaggtt accaggatga tttcgttgtt cagaacgcgc tgacgcagag cacctttgat
     1801 ttctgcttca gtcagttctt caccacccag gtatttttcc atcagctctt cagaagcttc
     1861 agctgcggat tcgatcaggt tctggtgcca ttcgttagcc agttcaacca tgtctgccgg
     1921 gatatcttcg tattcgaagg ttacgccctg gtcagcgtcg ttccagttga tagctttcat
     1981 tttcaccagg tcaacaacac cggtgaaatg ttcttcagca ccaatcgcca gctgcagcgg
     2041 aaccgggttc gcgcccagac gggttttgat ctggttaaca actttcagga agttcgcacc
     2101 catgcggtcc attttgttaa cgaacgcaat acgcggaact ttatatttgt ttgcctgacg
     2161 ccatacggtt tcagactgcg gctgaacacc accaactgcg cagtaaacca ttaccgcacc
     2221 atcgagaaca cgcatggaac gttctacttc gattgtgaag tcaacgtgcc ccggggtgtc
     2281 gatgatgttg atgcgatgcg gctcatactg cttagccata ccagaccaga atgcagtagt
     2341 cgcagcggaa gtgatggtaa taccacgttc ctgctcctgc tccatccagt ccatggttgc
     2401 agcgccgtca tgaacttcac cgattttatg gtttacaccg gtgtagaaca gaatacgttc
     2461 ggtagtagtg gttttaccgg cgtcgatgtg cgcactgata ccgatgttac ggtagcgtgc
     2521 gatgggtgtt gtacgagcca tttgtttcct cgtttatctt ttaggcgttc aatttaagta
     2581 gcccaaagcg ggctgcttac tggaagcgcc cgcctggtga ctaaaactcc gaagggatta
     2641 ccaacggtag tgtgcgaacg ccttgttggc ttcggccata cggtgaacgt cttcacgttt
     2701 cttaactgca gtacctttgt tttctgcagc atcagaaagt tcgttcgcca ggcgcagagc
     2761 catggattta tcaccgcgtt tacgagcagc ttcaacgatc caacgcattg ccagagcatt
     2821 acgacgaacc ggacggactt caactggtac ctgataagta gaaccaccaa cgcggcgaga
     2881 cttaacttct acagtcgggc gcacgttttc gagagctact tcgaatgctt ccagttcaga
     2941 tttaccagag cgctgagcca gggtctccag cgcgctgtat acgatagatt cagcagtaga
     3001 ttttttacca tctaccatca ggatatttac aaatttagcc agcagttctg atccgaactt
     3061 cggatccggc agaattttac gctgaccaat gacgcgacga cgtggcatgg aaatactccg
     3121 ttgttaattc aggattgtcc aaaactctac gagtttagtt tgacatttaa gttaaaacgt
     3181 ttggccttac ttaacggaga accattaagc cttaggacgc ttcacgccat acttggaacg
     3241 agcctgctta cggtctttaa cgccggagca gtcaagcgca ccacgtacgg tgtggtaacg
     3301 aacacccggg aggtctttaa cacgaccacc acggatcagg atcacggagt gctcctgcag
     3361 gttgtgacct tcaccaccga tgtaggaagt cacttcgaaa ccgttagtca gacgaacacg
     3421 gcatacttta cgcagcgcgg agttcggttt tttaggagtg gtagtatata cacgagtaca
     3481 tacgccacgt ttttgcgggc atgcttccag cgcaggcacg ttgcttttcg caactttgcg
     3541 agcacgtggt ttgcgtacca gctggttaac tgttgccatt aaatagctcc tggttttagc
     3601 ttttgcttcg taaacacgta ataaaacgtc ctcacacaat atgaggacgc cgaattttag
     3661 ggcgatgccg aaaaggtgtc aagaaatata caacgatccc gccatcacca ggccatctgg
     3721 ctggagtgct taaccgtaag tctgacgaaa tcagtatagt caatgggaat gatgtcgttc
     3781 gaaattcgac cagtcaaacc gcgggcaata aggtcttcgt tcagagcata gaccttaatg
     3841 ggggcattac gcagactttc aaggtagcgg ttgccgtcaa ctgcggcagt tacgccatct
     3901 tgcaataata gcagttcgtc accttcactg agcagacgca gcagcgcagc aaagtccgtc
     3961 agccagggtg aacgatgtaa tgtgtgcagc ataacgcctc aaaacctcaa aataacatcg
     4021 tagttggcga gttcgcggcg taaggcatct gcttcgagcg gcgtggcttc gaccacaaaa
     4081 ggtgtctgcg gatctaaccc gcgttcgcgc agtgaagccg cacaaaccca gcactgttca
     4141 atgtcataca gacccaacaa tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg
     4201 ggcttttgtc ctggcagcag ctgaaaaacg ccatcagcaa taaagaagac ggccagatcg
     4261 tcagttaatg cggaagttgc gagtaaagca tctaatccct cgcggccagc cgcggtgcca
     4321 tgaggcacag tagaaaaaac aaacgcaatt cgtttcatca gaactgtacc accctgtcac
     4381 aggtcagcga ggcttccgcc agcgcgccaa gtccgcttaa ggtaaatccc gtctgaaggt
     4441 ttgacgaagc cagccccagt cttccggctt ccgtttcatc aacaacgcca cggcgtaatg
     4501 ccgccgctac gcagatattc agcgccacac catgttgcat attcaactgt tgccagctgc
     4561 gcacaaggtc aaattcgtcg cttgccggtg aggtcaattg gttagcgtta tagacccctt
     4621 cccgatagaa aaaaacgctg cttaactcat ggccttctgc tattagcgcc tgcgcaaact
     4681 gaaaagcact actcgcctgt tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac
     4741 gcattactta tcttgcccct ggaaatcacc gctcttgaac tggcggatgt agagatagac
     4801 agtgtgtttg gagatgttca ggcggtcagc aacctggttg atcgcgtctt taatatcgaa
     4861 gatccctttc tcgtagagat ttagcacgat ctgacgattt ttggcgttat tagaaacatt
     4921 gcgatcggca ttcacttctt cgatggtaaa ctccagcgtt tgggtaacca gatcttcaac
     4981 agaagaggca aagttgacgc ttgaaccgac atccggagtt tccggcggca caaaagtgct
     5041 cataatctgc gagaagggaa catcaagatt catattgatg cacagcagac caattacacg
     5101 ctgttcgcgg ttacgaatcg cgatagtcag ggacttcatt aatacgccgc ttttggcgcg
     5161 agtaaagtag catttagaaa cgctgctatc cgctcccgtc atatcgtgca gcatacgtag
     5221 cgccaggtca gtaattggcg aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg
     5281 aatggctgaa cattttagat cctgcaaaga gtgcaaaacg atttcacagt gggagccaat
     5341 aagcatcgct aacccgtcca ccaccgcttc gtaggatttc agaatatcaa aatcggtctg
     5401 gtcaaaagga cgttgatcca gtaaatccaa ctcactggtt tcgttggtta aaagcgacct
     5461 ggacatgaaa aaaaacactc cttttcagga gcctgtcgtt aacttttcag ggcaggctca
     5521 ttaatgatgc gggtaactaa attaatacag cggaggttcc gctttccagc actaattata
     5581 tccggcctgt aataaaaaaa ccgccgcctg gtcaggcggc ggttcttaat gcttattttt
     5641 tagcagagtc tgcggctttc gcattagctt ccggctttgc atcagccttc ggcgctggtt
     5701 tcacatccag cagctctacg tcaaacacca gggtagaatt cggtgggatc cccggaacac
     5761 ccgctttgcc gtaagccagt tctggtggaa taaccagttt gatcttaccg cctttcttga
     5821 tgttcttcag accttctgtc caacccggga taacaccgtc cagacggaaa gagagcggtt
     5881 caccacgggt gtaagagttg tcgaactctt taccgtcgat cagcgtacct ttgtagttca
     5941 ctacaacagt atcgctgtct ttcggggctt cgcctttacc agcttctact acctgataaa
     6001 ccagaccagt ggaagaggtt ttcacacctt tctctttggc aaatttctcg cggtactctt
     6061 taccttttgc ttcgttatca gccgcgtctt tttccatctt cgcctgagca gaagacttca
     6121 cgcgagcttc gaatgcttgc agagtctgtt cgatctcttg gtcggaaagt ttgctcttat
     6181 cagcaaatgc atcctgaaca ccagcgatca gctgatcttt atccagtttg atgcccagtt
     6241 tttcttgttc tttaagagag ttttccatgt aacgacccag cgaagcaccc agtgcataag
     6301 ctgatttctg atcgtcattt ttgaacgctg ctttgctgtc agcagttgta gcaggttttg
     6361 cagcttcagc agcaaaagtg attggtgcat gcagggcaac ggccattgtg gtcgccagca
     6421 gcgttacttt aaacagtgat ttcatccata tctccaggat cggggcatct caccccaggg
     6481 ttaactatta tcagaagggt actataaagc gttgtcgaac aaatctacat acagacacgc
     6541 cctattatca tctattttca gactcttttt gtttaaatta gtttcgatga ccgcgaaatg
     6601 agtgctgtct cgggcagcaa agttaagtag aatccgcggc gaccattcga caaaagaggt
     6661 gaatcatgca ggatttatca ttggaagcac gcctggcaga gctggagagc cgattggctt
     6721 ttcaggaaat caccattgaa gaactgaacg tcacggtgac cgctcatgaa atggagatgg
     6781 cgaaactgcg cgatcatctg cgtctgctga ccgagaagct aaaagccagc cagccgtcga
     6841 acatcgcgtc gcaggctgaa gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg
     6901 ggattccccc gcttttttgt caccttttcg gtattagtgg caaccgcaac cgccgttgcc
     6961 tttaccgcca cagcagcctt cgccaccgtg ttcatgaccg tgatcatggc catgaccacc
     7021 gcagcaaccg tcgtggtcgt gatcgtggtg gtgatcgtgc gcgccgtgaa cgtgaccatg
     7081 agccagttct tcttcggtcg cttcgcgaat cgccacaact tcaacgttga atttcaggtt
     7141 ctgaccagcc agcatgtggt taccatcaac cactacgtga tcgtcttcaa ccgcagtgat
     7201 ttcaaccggt accggaccct ggtcggtttc agccaggaaa cgcataccta cctgcagttc
     7261 atcaacgccc ataaatacgt ctttaggaac gcgctgcacc aggttttcgt cgtactgacc
     7321 gtaagcgtcg ttcgcgccaa cagcgacatc aaatttgtcg ccaacttcat gaccttccag
     7381 cgccgtttcc aggccagaga tcagggaacc gtgaccatgc aggtagtcca gcggcgcact
     7441 caccggagac tcatcaacca acacaccgtc ttctgtacgt acctgatagg ccaggctgac
     7501 caccaggtct tttgctactt tcatgatatc tcctgagcat gggaagaata gtggcgcaga
     7561 ttgtagcgga attctgcagc cgtgtactca ctagcttaaa aaaactcggc gcatatcgct
     7621 agtccggatg aaaaatcccg atcacttgct catctttgcg gacgtgatcg cgggcttctt
     7681 tgtctgcttc tcgcatctga tgtccacact taacacattc tacgatatca atattatttt
     7741 cgcgccacat cgccattgaa tcctgtgcct gacaggccgg gcattttgcg cccgcaataa
     7801 aacgttttcg gattgccatc tttaccctct actcaaattc atcccagccg tccagctggc
     7861 gtcgttcttg ttgcatttca cgctggaaaa tctcttccag ttcacgtcgg gcttccctgg
     7921 cgcgagaaat ttgtacggta tcagcatgca tcgggattaa ctcccgcagc attcgcatat
     7981 ccaggcggcg aaaatgcagt tgcgcgcgct gcgcctgatg cggatgcatg ccaagcgtga
     8041 ccagcgtctt gcgccccagc tctaacgcac tggagaatgt ttcacgggaa aactgcgtca
     8101 cccctgcctg taataactca tgcgcttcca cacgtccgcg cgctcgcgca agaatatgca
     8161 aatgcggaaa gtgctgctgg catatttcca ccagcttcat agtgtcttcc ggctcgttac
     8221 aggtaatgac gatagactca gcggcctctg cacccgcaga acgtaaaaga tctacctgcg
     8281 tggcgtcgcc gtaataaact ttgtagccat atttgcgcat caggttaacg gcactgatat
     8341 cccgctccag tacagtaatg cgcattttat ttgccatcag caaacgacca atcacctgac
     8401 caaaacgccc aaagcccacg acaatgacct gcggtttatc atcgttgacc catggttttt
     8461 cgtcttcttc ttccggtccg ttaaactggc gggatagcca tttatcgacc agcttcatta
     8521 gcagcggcgt ggtcatcatg gaaagcgtca ccgtcaccag caacagcgcc atctggtcgc
     8581 cctggaataa gcgttgtgaa gaagcggtag aaaagaggac aaaggcaaac tcaccgccct
     8641 gactcaacac gccagcaaac tgcatccgct cagaactgcg cacgccatac aatcgcgcca
     8701 gcagatacag cacgagaatt ttcaccgcca ccagcacaac cacacttatc actacccaca
     8761 acagatgggt ataaagcacc ccgaggttga gcgacatgcc gacagagata aagaacaaac
     8821 caagcagcaa gcctttgaag ggatcgatag ccgtttccag ttcatggcga tattcacttt
     8881 ccgccagtag cacacccgca ataaacgtac cgagcgccat cgacaacccc agcgcatcca
     8941 taaacaatgc ggaacccaac accagcagca gcgtcgcggc ggtgaacact tcccgcacac
     9001 cagaagctgc aataaaacgg aataccggac gcagtaaata gcgcccgcca atcagcatgc
     9061 cgacaaacgc cagcaccttc atgccgatct tcatccagtc gaaatgttcg tctgccgaac
     9121 ccgccaacaa cggcactaac gccagtgccg ggatcaccgc cagatcctga aacagcagta
     9181 ccgagaaacc taactggccg gattcgctgc gattcatccc tttctcacgc atcaattgca
     9241 acgccattgc agttgaagac atcgcaaggc caatgccacc gaccaccgcc gcctgccatg
     9301 agaaatgggt cagcatcaat aatcctgcca gcaacgccgc gcttaacagc acctgtgccg
     9361 cgcctacgcc aaaaatcgaa cgccgcagtt gccaaagctt ggaaggattc aactcaaggc
     9421 cgatgataaa catcaggaat accacgccga gttccgaaaa gtggaggatc tcatcgacgt
     9481 cgctaataaa ccccagcccc cacgggccaa tcgcaatccc cgccagcaaa tatcccaaca
     9541 ctgcaccaat acccagccgc gatgccagcg gcaccgcagc caccgccgcg aagagaaaca
     9601 gcactcctgc gagtaaaaaa tcagaacctt ccatcagcgg cctcctggag acagcggatt
     9661 tgccagccag tcaccgtagg ctctggcgtg gctcgccagc tcctgtgcgc tttgccgtct
     9721 cgcccagtaa ataatgatgg ggcttaacca atgcatccgg cacatgcccg ccgccagttc
     9781 aaaggggcgc agaacatcgc tcatcgggta gcgattcaac gcgtcataac ggtaagcact
     9841 ttccggctcc ccggtggtaa tcacgctacg ccagtacttt cccgccagtt ggtttccccc
     9901 cggtccgctg gcaaaaccac ggcttaatac ccggtccagc cactctttca gtagcgccgg
     9961 gcagctatag gtataaagag gatgctgaaa gacaatcacc tcgtgctcgc gcagtaatgc
    10021 ctgctcacga gggatatcaa taaaaaagtc gggatagtgc gcgtaaaggt cgtgcacggt
    10081 aacattgctg agctgcgtgg ccggtttaag cagaacccgg tttgccaccg agtcctgaga
    10141 ttccggatgg gcatacagca gcaaaacttt cgctggctga gacatcatcc ccctcccggt
    10201 atggtttttg tgtatagtcg ctgttttggg ctaccattgc gcccggtgcg gcagctcgcc
    10261 catatattac attatcataa tgataagtta acatagtctg aacatacggc gccttatgat
    10321 tgttttctcc tcgttacaaa ttcgtcgcgg cgtgcgcgtc ctgctggata atgccaccgc
    10381 caccatcaac cccgggcaga aagtcggcct ggtgggtaaa aacggctgtg gtaaatcgac
    10441 cctgctggca ttgctgaaaa atgaaatcag cgccgacggc ggcagctaca cctttccggg
    10501 aagctggcaa ctggcgtggg tgaatcagga aacaccggcg ttaccgcaag cggcgctgga
    10561 atatgtcatt gacggcgacc gtgaatatcg tcaactggaa gcgcagctac acgacgccaa
    10621 cgaacgtaac gacgggcacg ccatcgccac tattcatggc aagctggatg ctattgacgc
    10681 atggagtatt cgctcccgtg ccgccagcct gctgcacggc ctcggtttca gcaatgaaca
    10741 actggagcgc ccggtaagtg atttctccgg tggctggcgt atgcgtctta accttgccca
    10801 ggcgctgatt tgccgttcag acttgctgct gctcgacgaa ccgactaacc acctcgatct
    10861 cgatgccgtt atctggctgg aaaaatggct gaagagctat cagggcacgc tgatcctgat
    10921 ctctcacgac cgcgacttcc tcgatccgat cgttgataaa attattcata tcgaacaaca
    10981 aagcatgttc gagtacaccg gcaactacag ttcgtttgaa gtacagcgcg ccacccgtct
    11041 ggcgcagcaa caagcgatgt acgaaagcca gcaggaacgc gtagcgcatc tgcaaagtta
    11101 tatcgaccgt ttccgtgcca aagccaccaa agcgaagcag gcccagagcc gtattaagat
    11161 gctggagcgt atggagctga ttgccccggc gcacgtcgac aacccgttcc gctttagctt
    11221 ccgcgcgccg gaaagcctgc ctaatccgtt attaaagatg gaaaaagtaa gcgcaggcta
    11281 tggtgatcgc attattctcg actcgattaa actgaatctg gtccccggct cgcgcattgg
    11341 tctgctaggc cgcaacggcg cgggtaaatc gacattaatc aaactgttag ccggtgaact
    11401 tgcgccagtc agcggtgaaa ttggtctggc gaaagggatc aagctcggct acttcgccca
    11461 gcatcaactt gaatacctgc gcgccgacga atcgcctatt caacatctgg cacgtttagc
    11521 gccgcaggag ctggaacaaa aactgcgtga ttacctcggc ggctttggtt tccagggcga
    11581 taaagtaacc gaagaaacgc gccgcttttc cggtggggaa aaagcccgcc tggtgctggc
    11641 attaattgtc tggcagcgtc cgaatctgct gctgctcgac gaaccgacca accaccttga
    11701 cctcgacatg cgtcaggcac tcaccgaagc attaatcgag ttcgaaggcg cgctggttgt
    11761 cgtctcgcac gaccgtcatt tgctgcgttc caccactgac gatctctacc tggttcacga
    11821 tcgtaaagtc gaaccgttcg acggcgatct ggaagattat caacagtggc tgagcgacgt
    11881 acaaaagcag gaaaaccaga ccgacgaagc gccaaaagag aacgcgaaca gcgcccaggc
    11941 acgtaaagat cagaagcgtc gggaagcgga gctgcgtgcg caaacccagc cactgcgtaa
    12001 agagattgcc cgtctggaaa aagagatgga gaagctgaac gcgcaactgg cgcaggcgga
    12061 agagaaactc ggcgacagcg aactgtatga ccagagccgt aaagcggagt tgaccgcctg
    12121 cctgcaacag caagccagcg ccaaatccgg cctggaagag tgcgaaatgg cgtggctgga
    12181 agcccaggag cagcttgagc agatgctgct ggaaggccaa agcaactgat ggcgcagata
    12241 acaacgaccg atgccaatga atttagcagc agtgctgaat tcacccctat gcgcggcttt
    12301 agcaattgtc atctgcaaac catgctgccg cgtctgtttc gtcgcaaggt gaaattcacc
    12361 ccgcactggc agcgactgga gttgcccgac ggcgattttg tcgatctcgc gtggagtgaa
    12421 gaccccgcac aggcgaaaca taaaccgcgt ttagtggtgt ttcacgggct ggaaggcagt
    12481 ctcaatagcc cttacgccca cggtctggtt gaggcggcgc aaaagcgcgg ctggctgggc
    12541 gtggtgatgc attttcgcgg atgtagcggt gaaccaaacc gtatgcaccg catttaccat
    12601 tcgggcgaaa ccgaagacgc cagttggttt ttacgctggc tgcaacgcga gtttggtcat
    12661 gcgccaacgg ctgccgtcgg ctattcgctc ggcggtaata tgctggcctg tttgctagca
    12721 aaagaaggca atgatctccc gattgatgcg gcggtgattg tctctgcgcc gtttatgctg
    12781 gaagcctgta gctatcatat ggaaaagggc ttttcccgcg tttatcagcg ttacttgctg
    12841 aacctgttaa aagccaatgc cgcgcgcaag ctggcagcct accccggaac actgccgatt
    12901 aatctcgcgc agttaaaatc ggtacgtcgc atccgtgaat ttgacgatct catcaccgcc
    12961 agaattcacg gctacgccga cgctatcgac tattatcgtc agtgtagcgc catgccgatg
    13021 ctcaatcgga tagccaaacc gacgctgatt attcacgcca aagacgatcc gtttatggat
    13081 catcaggtga tcccgaaacc ggaaagtctc cccccgcagg tggagtatca actgactgaa
    13141 catggcggtc atgttggctt tattggcggt acattacttc atccgcaaat gtggctggag
    13201 tcacgcattc ctgactggtt aacaacgtat ctggaggcga aatcatgttg attccgtggc
    13261 aagacctctc ccccgaaacg ctggaaaatt taattgaaag ctttgtgtta cgtgaaggca
    13321 ccgattatgg tgaacatgag cgtacacttg aacagaaagt cgccgacgtc aaacgccagc
    13381 tacagtgcgg agaagcggtg ctggtatggt cggagctgca cgaaacggtc aatatcatgc
    13441 cgcgcagtca gtttcgcgaa taacctgcaa ccgtcggcgg acgagataat aatgatagct
    13501 aagtcatgga gttactatgt ctgccaaaca tccggtcatt gcggtaacag gatccagcgg
    13561 cgcggggacc accaccacca gcctcgcgtt tcgtaaaata ttcgcacagt taaatctgca
    13621 tgcagccgag gtggaaggcg acagttttca ccgttatacc cgcccggaaa tggacatggc
    13681 gatccgcaaa gcgcgcgacg ccgggcggca tatcagctac ttcggccccg aagcgaacga
    13741 cttcggcctt ctggaacaaa ccttcattga atacggtcag agcggcaaag ggaaatctcg
    13801 caaatatctg catacctacg atgaagccgt accgtggaat caggtaccgg ggacattcac
    13861 cccctggcaa tctttaccgg aacccactga tgtactgttt tatgaaggtt tacacggcgg
    13921 cgtagtcacg ccacagcata acgttgcgca gcatgtggac ttactggtcg gcgtggtgcc
    13981 tatcgttaac cttgagtgga ttcaaaaact gatccgcgac accagcgagc gcgggcactc
    14041 acgagaagca gtgatggact cggtagtgcg ttcaatggaa gactatatca actacatcac
    14101 accgcagttt tcccgcaccc atcttaactt ccagcgcgtt cccaccgtcg acacctcaaa
    14161 cccgttcgcg gcaaaaggta tcccgtcgct ggatgaaagc tttgtggtga tccattttcg
    14221 taatctggaa gggatcgatt tcccctggct gctggcgatg ttgcaaggct cattcatttc
    14281 ccacatcaat acgttagtgg tgcctggcgg caaaatgggt ctggcaatgg aattaattat
    14341 gctgccgctg gtgcaacgat tgatggaagg aaagaaaatc gagtaacgct gctattacgc
    14401 cggataaaat actatccggc ttcacaacgg gatagttaaa tcacgcggca accacttcat
    14461 acgagtgagt aatattcacc gctttttcca gcatcagcgc cactgaacaa tatttctcgg
    14521 cagagagatc aaccgcacgc gcaaccgctg cgtctttcag gtcacgaccg gtgacgataa
    14581 aatgcagatt aatgtgcgta aacaggcgtg gtgcctcttc gcggcgctca gaggtcaatt
    14641 ttacttcaca atcgaccaca tcctgacgcc ctttttgcag gatagaaacc acatcgatgg
    14701 cactgcaacc acccgctgcc atcaacacca tttccatcgg gcttggtgct ttatcgcctg
    14761 agttgccgtc cattaaaatc tggtgaccag aggcggattc gcccagaaaa gttaaccctt
    14821 cgacccactt cactcgcgct tgcatttttg ctactccact gcgtcaattt tcctgacaga
    14881 gtacgcgtac taaccaaatc gcgcaacgga aggcgacctg ggtcatgctg aagcgagaca
    14941 ccaggagaca caaagcgaaa gctatgctaa aacagtcagg atgctacagt aatatattga
    15001 tgtactgcat gtatgcaaag gacgccacat taccgtgcag tacagttgat agccccttcc
    15061 caggtagcgg gaagcatatt tcggcaatcc agagacagcg gcgttatctg gctctggaga
    15121 aagcttataa cagaggataa ccgcgcatgg tgcttggcaa accgcaaaca gacccgactc
    15181 tcgaatggtt cttgtctcat tgccacattc ataagtaccc atccaagagc acgcttattc
    15241 accagggtga aaaagcggaa acgctgtact acatcgttaa aggctctgtg gcagtgctga
    15301 tcaaagacga agagggtaaa gaaatgatcc tctcctatct gaatcagggt gattttattg
    15361 gcgaactggg cctgtttgaa gagggccagg aacgtagcgc atgggtacgt gcgaaaaccg
    15421 cctgtgaagt ggctgaaatt tcgtacaaaa aatttcgcca attgattcag gtaaacccgg
    15481 acattctgat gcgtctgtct gcacagatgg cgcgtcgtct gcaagtcact tcagagaaag
    15541 tgggcaacct ggcgttcctc gacgtgacgg gccgcattgc acagactctg ctgaatctgg
    15601 caaaacaacc ggatgctatg actcacccgg acggtatgca aatcaaaatt acccgtcagg
    15661 aaatcggtca gattgtcggc tgttctcgtg aaaccgtggg acgcattctg aagatgctgg
    15721 aagatcagaa cctgatctcc gcacacggta aaaccatcgt cgtttacggc actcgttaat
    15781 cccgtcagag tggcgcgtta cctggtagcg cgctattttg tttcccccga tgtggcgcag
    15841 actgatttat caccccgata tcaactatgc acttcgacaa acgctggtgc tatgtttgcc
    15901 cgtggccgtt gggttaatgc ttggcgaatt acgattcggc ctgctcttct ccctcgttcc
    15961 tgcctgttgc aatattgcgg gccttgatac gcctcataaa cgttttttca aacgcttaat
    16021 cattggtgcg tcgctgtttg ccacctgtag cttgctgaca caggtgctac tggcaaaaga
    16081 tgttcccctg ccctttttgc tgaccggatt aacgctgata cttggcgtca ctgctgagct
    16141 ggggccattg cacgcaaaat tgcttcccgc atcgctgctc gctgccattt ttaccctcag
    16201 tctggcggga tacatgccgg tctgggaacc gttgctcatc tatgcgctgg gcactttgtg
    16261 gtacggattg tttaactggt tttggttctg gatctggcgc gaacaaccgc tacgcgagtc
    16321 actaagtctg ctgtaccgtg aactggcaga ttactgtgaa gccaaataca gcctgcttac
    16381 ccagcacacc gaccctgaaa aagcgctgcc gccgctgctg gtgcgccagc aaaaagcggt
    16441 cgatttaatt acccagtgct atcagcaaat gcatatgctt tccgcgcaaa ataataccga
    16501 ttacaagcgg atgctgcgta ttttccagga ggcgctggat ttgcaggaac atatttcggt
    16561 cagtttgcat cagccggaag aggtgcaaaa gctggtcgag cgtagccatg cggaagaagt
    16621 tatccgctgg aatgcgcaaa ccgtcgccgc tcgcctgcgc gtgctggctg atgacattct
    16681 ttaccatcgc ctgccaacgc gttttacgat ggaaaagcaa attggcgcac tggaaaaaat
    16741 cgcccgccaa cacccggata atccggttgg gcaattctgc tactggcatt tcagccgcat
    16801 cgctcgtgtg ctgcgcaccc aaaaaccgct ctatgcccgt gacttactgg ccgataaaca
    16861 gcggcgaatg ccattacttc cggcgctgaa aagttatctg tcactaaagt ctccagcgct
    16921 acgcaatgcc ggacgactca gtgtgatgtt aagcgttgcc agcctgatgg gcaccgcgct
    16981 tcatctgccg aagtcgtact ggatcctgat gacagtattg ctggtgacgc aaaatggcta
    17041 tggcgcaacc cgtctgagga ttgtaaatcg ctccgtggga accgtggtcg ggttaatcat
    17101 tgcaggcgtg acgctacact ttaaaattcc cgaaggttac accctgacct tgatgctgat
    17161 taccaccctc gccagctacc tgatattgcg caaaaactac ggctgggcga cggtcggttt
    17221 tactattacc gcagtgtata ccctgcaact gttgtggctg aacggtgagc agtacatcct
    17281 tccgcgtctt atcgatacca ttattggttg tttaattgct ttcggcggta ctgtctggct
    17341 gtggccgcag tggcagagcg ggttattgcg taagaacgcc catgacgctt tagaagccta
    17401 tcaggacgcg attcgcttga ttctgagcga ggacccgcaa cctacgccac tggcctggca
    17461 gcgaatgcgg gtaaatcagg cgcataacac tctgtataac tcattgaatc aggcgatgca
    17521 ggaaccggcg tttaacagcc attatctggc agatatgaaa ctgtgggtaa cgcacagcca
    17581 gtttattgtt gagcatatta atgccatgac cacgctggcg cgggaacacc gggcattgcc
    17641 accggaactg gcacaagagt atttacagtc ttgtgaaatc gccattcagc gttgtcagca
    17701 gcgactggag tatgacgaac cgggtagttc tggcgatgcc aatatcatgg atgcgccgga
    17761 gatgcagccg cacgaaggcg cggcaggtac gctggagcag catttacaac gggttattgg
    17821 tcatctgaac accatgcaca ccatttcgtc gatggcatgg cggcagcgac cgcatcacgg
    17881 gatttggctg agtcgcaagt tgcgggattc gaaggcgtaa tacaggctaa accatcggat
    17941 gaggtgttca cgccgcatcc gacatctttt gcctgacgcg tcttatttac gccccaatca
    18001 ccttcgccac cgcgtgggcg aaacgatgca tcccttcatc gatatccgca tcttccacca
    18061 ccagcgacgg cgcaaaacgc atcacatcag gcccggcatt cagcaccatt gcgccttcct
    18121 ctgcaccggc atacaggaaa tcacgcgccc gacctttgta ctgtggtttc agctctgcgc
    18181 caatcaacag ccccataccg cgaatatcgc taaacacatc gtactgctga tcgatcttct
    18241 gcaaatggtc aacaaaacgc tggcgtttcg cctgaatacc ttccagcact tcaggggtat
    18301 tgatgatatc aaacgctgcg cccgctactg cacaggccag aggattaccg ccgtaggtgg
    18361 aaccgtgaga accaggatga aacgcggaag caatctccgc cgtggtcagc atggcgctaa
    18421 ccgggaagcc gccacctagc gctttcgcag aggtcagaat atccggcgtc acgccgtagt
    18481 gcatataagc aaacagatcg ccggtccgcc ccatcccgca ctgcacttca tcaaacacca
    18541 ataatgcctg atgttgatcg cacagctcgc gcaagccctg caaaaactct ggcgtcgctg
    18601 ccgtcacgcc gccctcgccc tgaatcggct caaccaccac cgcacaggta tggtcatcca
    18661 tcacggcttt tactgcgtga agatcgttaa aaggcacgtg gatgatgtct gccggttttg
    18721 gcccaaagcc gtcggaatat tttggctgcc cacccaccga aacggtaaac agcgagcgac
    18781 catgaaaagc gttatggaag gcaataattt tggttttgaa cgggctatga cgcacacagg
    18841 cgtaatggcg tgccagttta aaggcggttt cgttagcttc cgtgccggag ttcataaaca
    18901 ccacgcgttc ggcaaacgtt gcctcaatca gtttacggcc aagacgcagc gccggctcat
    18961 tggtgaaaac gttactaata tgccacagag tttcgccctg ggtttttaac gcgttcacca
    19021 gcgcaggatg gcaatggccc aacgccgtaa ctgcaatgcc acccgcgaaa tcgacatact
    19081 ccttgccctg ttgatcccag attcggctgc cctgaccttt taccggaata aactctgccg
    19141 gagcataaat cggcagaatc acttcatcga aagtcgcgcg tgtaattgct gtttgttcaa
    19201 ttgccatctc atgatcaccc tgttacgcat aaataaatat gaaattataa tcacaaaata
    19261 tgcataaaaa atcactgaat ggcaatcaga aatcagcgat gcagaaaatt agccagcagt
    19321 tgatgtcctt gttcgctaag aatactttct ggatggaatt gcacaccttc cagatcccac
    19381 tggcgatggc gaatccccat aatctcgcgg gtttcgctcc aggccgtcac ttcaaagcac
    19441 tccggtaatg agtcaggttc caccaccagc gaatggtagc gtgtcacggt gagtggattt
    19501 gccagcccct taaatacgcc cacaccatta tgtgtaatcg gcgaggtttt gccgtgcatg
    19561 acctttgcgg cgcgcacaac tttgccgcca aatgcctgcg ccattgcctg atgaccgagg
    19621 cagacgccaa gaatcggcaa gcgtccggca tagtgacgaa taacgtcaag ggagatcccg
    19681 gcttcatctg gcgtacaggg gccgggtgag atgacaattt tttgtggttt aagggcgtcg
    19741 atatccgcca acgtcaacgc atcgttgcgc ttaaccaaca catccgcccc cagttcacaa
    19801 aagtactggt agaggttcca ggtaaaagaa tcgtagttat caataagcag gatcatggcg
    19861 gctccggtac gaaagaaccg cgctatttta ctcagattcc ccggcttcgc ttaccacttt
    19921 gctaaatatc atctgcagcg cggtgagatc ccccattgcc ccattctgat ttgcctgatt
    19981 ccaggcctct ttttcgatac cctgccagct cagttgatat ccggcatgaa tcgccagttg
    20041 ctcgaagaag atccgctgtg ccaaaccact tcccacccgg aagggatgca gcacgttgat
    20101 ttcacaatag taatgcgcca gccgctcgac gaacttcgct ttctccaggc caaccagata
    20161 accttcttcc tccagatcct gcatcagggc attgccctct ttttcgatat aagcaaagtg
    20221 gcagaacggc gtatcgccct gataaatatc aacttcacgc agttgccctg cccagtcgaa
    20281 aatatcctga tacagctggc gatggatagt gcgcaaatgc ggtaaaccgc gcaccagcgg
    20341 accaagctca atggtcgcag cacgcagcgc cgtcatttcg taagcggcct gttccagccg
    20401 ctgctgctgg cggatgttca gccggttacg catgatatca aggcctggat aaagatacgg
    20461 atcgcgccct tcgccgaatt tatcgctcat agtgcctcct cagcgcttca aggcgcgcta
    20521 aagcctctgc ggcagtaaga gtgactaaag gcatctcgac gccttcaaga cggcgactgg
    20581 cctggaaatt acgattacgc tgaagctccc agagacggga cttttgctta tcggtgagtt
    20641 tcttcacttg atgcctccct gaacgtgtcc atttgccaca agtataagcg gcaaatccgg
    20701 gttacgccgg gagagcagaa gcgcgggcaa gattgcccgc gcgagaaatc attacggcag
    20761 gactttagcg gaaaggataa ctaccggttt tgacggcaca ttctggtacg gaccaacgtc
    20821 atgagtcggc acctgggaaa tcttatcggc aacgtccatg cctttcacca ctttaccaaa
    20881 taccgcgtaa ccgaaatcac gctgaccgtg gtcaaggaag gcgttatcgg caacgttgat
    20941 aaagaactgg ctggtggcgc tgtctttgtc agcggtacgc gccatcgcga tggtgccacg
    21001 cgtgttgcgc aggccgttat ctgcttcatt tttgattggc gggtttggtt ttttctgctg
    21061 catctgctcg gtgaaaccgc cgccctgaat cataaagcca ggaatgacgc ggtgaaaggt
    21121 cgtgttgtta taaaagccac tgttcacata atcgacaaag ttttgcacag acactggcgc
    21181 tttttgttta tccagctcca gttcgatgtt accagctgag gttgtcaaca atacgtgcgg
    21241 gtcccctttc gctgccattg ctgcgggaga aagagcagaa agagcgaaaa cagcagccat
    21301 cgccgccagg gtcgatttga acatgagatt tccttaacaa acagagcaga aaaaaagcaa
    21361 gtgaagagat tctaaatagc cttcaagaac aaggccatcc ctttacctaa ttttacgtat
    21421 ctgaaatata tgtaacctga cgatcacttt ttattctgtg atcaaaatca cctcttaaaa
    21481 tgcaatttag caaccgattg caataaaaca tttaatcaga tcacaaaatc acctaaaatc
    21541 gcccgctcgc ggcatagccc gctatgccat tttatttttg aacgcaggcc aatcatgact
    21601 aacagcaatc gcatcaagct cacatggatt agctttctct cctacgcact gaccggtgcg
    21661 ttggttattg tcaccgggat ggtgatggga aatatcgccg attatttcaa tctgcctgtt
    21721 tccagtatga gtaacacctt caccttcctc aacgccggca ttttaatctc tatcttcctc
    21781 aacgcctggc tgatggaaat cgtcccgttg aaaacgcagt tacgttttgg ctttctcctg
    21841 atggtgctgg cggttgccgg tttgatgttc agtcacagcc tggcgctgtt ctcggcggcg
    21901 atgttcattc tcggggtggt cagcggcatc accatgtcga ttggtacatt cctgataaca
    21961 caaatgtatg aagggcgtca gcgcggttcc cgcctgttat ttaccgactc cttcttcagt
    22021 atggccggga tgattttccc aatgatcgcc gcgtttctac tggcgcgcag cattgagtgg
    22081 tactgggttt atgcctgcat cgggctggtg tatgtcgcta tttttattct gaccttcggt
    22141 tgtgagttcc cggcactggg caaacatgca ccaaaaacgg atgctccggt agcgaaagaa
    22201 aaatggggga tcggcgtgct gtttctctct gttgcggcgc tgtgctacat cctcggtcag
    22261 ttaggtttta tctcctgggt gccggagtat gccaaaggcc tgggcatgag cctgaacgac
    22321 gctggcacgc tggtgagtaa cttctggatg tcgtacatgg tcggcatgtg ggcgttcagc
    22381 tttattcttc gcttctttga tttgcagcgc attctgaccg tactggctgg tctggctgcg
    22441 attctgatgt acgtctttaa caccggaaca ccggcacata tggcgtggtc aattctcgcc
    22501 ctgggcttct tctccagcgc gatctatacc accatcatca ccctgggttc acagcagacc
    22561 aaagtaccgt cgccaaaact ggttaacttt gtcctgacct gcgggaccat cggtactatg
    22621 ttgacctttg tggttaccgg cccgattgtt gaacatagtg gtccgcaggc ggcgctgctg
    22681 acggcaaacg gtctgtacgc tgtcgtcttt gtgatgtgct tcctgttagg tttcgtcagc
    22741 cgtcaccgtc agcataacac cctgacctct cattaattgt tcatgccggg cggcactatc
    22801 gtcgtccggc cttttcctct cttactctgc tacgtacatc tatttctata aatccgttca
    22861 acttgtctgt tttttgcaca aacatgaaat gtcagacaat tccgtgactt aagaaaattt
    22921 atacaaatca gcaatatacc ccttaaggag tatataaagg tgaatttgat ttacatcaat
    22981 aagcggggtt gctgaatcgt taaggtaggc ggtaatagaa aagaaatcga ggcaaaaatg
    23041 agcaaagtca gactcgcaat tatcggtaac ggtatggtcg gccatcgctt tatcgaagat
    23101 cttcttgata aatctgatgc ggccaacttt gatattaccg ttttctgtga agaaccgcgc
    23161 atcgcttatg accgcgtaca cctctcgtct tacttctctc accacaccgc cgaagagctg
    23221 tcgctggtgc gcgaaggctt ctacgagaaa cacggcatca aagttctggt cggcgaacgc
    23281 gctatcacca tcaaccgtca ggagaaggtg attcactcca gcgccggacg taccgttttt
    23341 tatgacaagc tgatcatggc gaccggttcc tacccgtgga tcccgccaat taaaggttct
    23401 gatactcagg actgctttgt ctatcgcacc attgaagacc tcaacgccat tgaatcctgc
    23461 gctcgtcgca gcaagcgcgg tgccgttgtt ggtggcggcc tgttaggtct ggaagctgct
    23521 ggcgcgctga aaaacttagg cattgaaacc cacgttatcg aatttgcccc tatgctgatg
    23581 gcggaacagc ttgatcagat gggcggcgag cagctgcgtc gcaaaatcga aagtatgggc
    23641 gtgcgcgttc acaccagcaa aaacactctt gagattgtgc aggaaggtgt tgaagcgcgt
    23701 aaaaccatgc gttttgccga cggcagcgaa ctggaagtcg actttatcgt cttctctacc
    23761 ggtatccgtc cgcgcgataa gctggcaacc cagtgtggtc tggacgttgc tccgcgtggg
    23821 ggtatcgtca taaatgattc ctgccagact tccgatccgg atatctacgc catcggtgaa
    23881 tgcgcaagct ggaacaaccg tgtatttggt ctggtagcac caggctacaa aatggcgcag
    23941 gtcgccgttg accatattct tggtagcgaa aacgcctttg aaggtgcaga ccttagcgcc
    24001 aagctgaaac tgctgggcgt agacgtaggc ggtattggtg atgcgcacgg tcgcacgcct
    24061 ggcgcacgta gctacgttta cctcgacgaa agcaaagaga tctacaaacg cctgattgtc
    24121 agcgaagaca acaaaaccct gctcggtgcg gtactggtgg gcgataccag cgactacggc
    24181 aacctgctgc aactggtgct gaacgctatc gagctgccgg aaaacccgga ttccctgatc
    24241 ctgccagcgc actcgggtag cggtaagccg tctatcggtg ttgataaact gccggacagc
    24301 gcgcaaatct gctcctgctt cgacgtcacc aaaggcgacc tgattgctgc tatcaacaaa
    24361 ggctgccaca ccgttgcggc gctgaaagcc gaaaccaaag ccggtacggg ctgcggcggt
    24421 tgtattccgc tggtcactca ggtactgaac gcggaactgg cgaaacaggg tatcgaagtt
    24481 aacaacaacc tgtgtgaaca ctttgcttat tcgcgtcagg aactgttcca tttgatccgc
    24541 gttgaaggca ttaaaacctt cgaagaactg ctggcgaaac acggcaaagg ttacggttgt
    24601 gaagtttgta aaccgaccgt cggttcgctg ctggcctcct gctggaacga atacattctg
    24661 aagccggaac atactccgct gcaggattct aacgacaact tcctcgcgaa catccagaaa
    24721 gacggcacct actcggtgat cccgcgttct ccgggcggtg aaatcacccc ggaagggctg
    24781 atggcagtgg gtcgtatcgc gcgtgaattt aatctctaca ccaaaatcac cggctctcag
    24841 cgcctggcga tgtttggcgc acagaaagac gatctgccag agatctggcg tcaactgatt
    24901 gaagccggct tcgaaaccgg tcatgcctat gcgaaagcac tgcgtatggc gaaaacctgc
    24961 gtgggaagca cctggtgccg ctacggtgtt ggcgacagcg tcggcctcgg cgtggaactg
    25021 gaaaaccgtt acaaaggcat ccgtacgccg cacaaaatga agttcggtgt ctccggctgt
    25081 acccgtgaat gttcagaagc tcagggtaaa gacgtgggta ttatcgccac cgaaaaaggc
    25141 tggaacctgt acgtttgcgg taacggcggc atgaaaccgc gtcatgcgga tctgctggca
    25201 gcggatatcg atcgcgaaac gctgatcaaa tatcttgacc gcttcatgat gttctacatc
    25261 cgtactgccg acaaactgac gcgtaccgcg ccgtggttag aaaacctcga aggtggcatc
    25321 gattacctga aagcggtgat cattgacgac aaactggggc tgaacgctca tctggaagaa
    25381 gagatggcgc gcctgcggga agcggtagtg tgcgaatgga ctgaaacggt caatacgccg
    25441 tctgcgcaga ctcgcttcaa acacttcatc aacagcgaca agcgtgaccc gaacgtgcag
    25501 atggtgccag agcgcgaaca gcaccgtccg gcaacgccgt atgaacgtat cccggtaact
    25561 ctggtggagg acaacgcatg agccagtgga aagacatctg caaaatcgat gacatcctgc
    25621 ctgaaaccgg cgtctgcgcg ctgttaggtg acgaacaagt agcaattttc cgcccgtatc
    25681 acagcgatca ggtgtttgcg atcagcaaca tcgacccgtt cttcgagtcc agcgtgctgt
    25741 cacgcgggtt gattgcggaa caccagggcg agctgtgggt cgccagtccg ctgaaaaagc
    25801 agcgttttcg cttaagcgac ggcttgtgca tggaagacga acagttttcc gtcaagcatt
    25861 acgacgcgcg agtgaaagac ggcgtggtac agctgcgcgg ttaatatttt aacgggaggc
    25921 gcaatgcctc
//
LOCUS       DADSZD010000057        25814 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.057, whole genome shotgun sequence.
ACCESSION   DADSZD010000057 DADSZD010000000
VERSION     DADSZD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.057"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(49..129)
                     /gene="shoB"
                     /locus_tag="J1Z28_002070"
     CDS             complement(49..129)
                     /gene="shoB"
                     /locus_tag="J1Z28_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBA7716905.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(550..930)
                     /locus_tag="J1Z28_002071"
     CDS             complement(550..930)
                     /locus_tag="J1Z28_002071"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBA7716906.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(930..1661)
                     /gene="pdxJ"
                     /locus_tag="J1Z28_002072"
     CDS             complement(930..1661)
                     /gene="pdxJ"
                     /locus_tag="J1Z28_002072"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBA7716907.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGILVSLFIDADEDQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1673..2401)
                     /gene="recO"
                     /locus_tag="J1Z28_002073"
     CDS             complement(1673..2401)
                     /gene="recO"
                     /locus_tag="J1Z28_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBA7716908.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGVTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2413..3318)
                     /gene="era"
                     /locus_tag="J1Z28_002074"
     CDS             complement(2413..3318)
                     /gene="era"
                     /locus_tag="J1Z28_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBA7716909.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3315..3995)
                     /gene="rnc"
                     /locus_tag="J1Z28_002075"
     CDS             complement(3315..3995)
                     /gene="rnc"
                     /locus_tag="J1Z28_002075"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBA7716910.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4267..5241)
                     /gene="lepB"
                     /locus_tag="J1Z28_002076"
     CDS             complement(4267..5241)
                     /gene="lepB"
                     /locus_tag="J1Z28_002076"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBA7716911.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5257..7056)
                     /gene="lepA"
                     /locus_tag="J1Z28_002077"
     CDS             complement(5257..7056)
                     /gene="lepA"
                     /locus_tag="J1Z28_002077"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBA7716912.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7254..7733)
                     /gene="rseC"
                     /locus_tag="J1Z28_002078"
     CDS             complement(7254..7733)
                     /gene="rseC"
                     /locus_tag="J1Z28_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBA7716913.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7730..8686)
                     /gene="rseB"
                     /locus_tag="J1Z28_002079"
     CDS             complement(7730..8686)
                     /gene="rseB"
                     /locus_tag="J1Z28_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBA7716914.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(8686..9336)
                     /gene="rseA"
                     /locus_tag="J1Z28_002080"
     CDS             complement(8686..9336)
                     /gene="rseA"
                     /locus_tag="J1Z28_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBA7716915.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9369..9944)
                     /gene="rpoE"
                     /locus_tag="J1Z28_002081"
     CDS             complement(9369..9944)
                     /gene="rpoE"
                     /locus_tag="J1Z28_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBA7716916.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9941..10096)
                     /gene="rseD"
                     /locus_tag="J1Z28_002082"
     CDS             complement(9941..10096)
                     /gene="rseD"
                     /locus_tag="J1Z28_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBA7716917.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10352..11974
                     /gene="nadB"
                     /locus_tag="J1Z28_002083"
     CDS             10352..11974
                     /gene="nadB"
                     /locus_tag="J1Z28_002083"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBA7716918.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGEESYHLTREGGHSHRRILHAADATGREVETTLVGKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRIANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDISRRIPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSAGNHYINR"
     gene            complement(11959..12696)
                     /gene="trmN"
                     /locus_tag="J1Z28_002084"
     CDS             complement(11959..12696)
                     /gene="trmN"
                     /locus_tag="J1Z28_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBA7716919.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADILQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12828..14162
                     /gene="srmB"
                     /locus_tag="J1Z28_002085"
     CDS             12828..14162
                     /gene="srmB"
                     /locus_tag="J1Z28_002085"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBA7716920.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14195..15076)
                     /locus_tag="J1Z28_002086"
     CDS             complement(14195..15076)
                     /locus_tag="J1Z28_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716921.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQPLALVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVVKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFLPG"
     gene            15179..15766
                     /gene="eamB"
                     /locus_tag="J1Z28_002087"
     CDS             15179..15766
                     /gene="eamB"
                     /locus_tag="J1Z28_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBA7716922.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(15822..16205)
                     /gene="grcA"
                     /locus_tag="J1Z28_002088"
     CDS             complement(15822..16205)
                     /gene="grcA"
                     /locus_tag="J1Z28_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBA7716923.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            16510..17199
                     /gene="ung"
                     /locus_tag="J1Z28_002089"
     CDS             16510..17199
                     /gene="ung"
                     /locus_tag="J1Z28_002089"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBA7716924.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(17247..18284)
                     /locus_tag="J1Z28_002090"
     CDS             complement(17247..18284)
                     /locus_tag="J1Z28_002090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBA7716925.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQHRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNMLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            18491..18910
                     /gene="trxC"
                     /locus_tag="J1Z28_002091"
     CDS             18491..18910
                     /gene="trxC"
                     /locus_tag="J1Z28_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBA7716926.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKGRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            18979..19677
                     /locus_tag="J1Z28_002092"
     CDS             18979..19677
                     /locus_tag="J1Z28_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DTW domain-containing protein"
                     /protein_id="HBA7716927.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGNPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTRAATGLGEHFTRFKTRYLAGKT
                     QHPGSITAEQLESV"
     gene            19709..22369
                     /gene="pat"
                     /locus_tag="J1Z28_002093"
     CDS             19709..22369
                     /gene="pat"
                     /locus_tag="J1Z28_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBA7716928.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLSLAQREES"
     gene            22483..23838
                     /gene="pssA"
                     /locus_tag="J1Z28_002094"
     CDS             22483..23838
                     /gene="pssA"
                     /locus_tag="J1Z28_002094"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBA7716929.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELDLIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            23884..24207
                     /locus_tag="J1Z28_002095"
     CDS             23884..24207
                     /locus_tag="J1Z28_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBA7716930.1"
                     /translation="MRILFVCSLLLLSGCSHMANDNWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(24204..25502)
                     /gene="kgtP"
                     /locus_tag="J1Z28_002096"
     CDS             complement(24204..25502)
                     /gene="kgtP"
                     /locus_tag="J1Z28_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBA7716931.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
BASE COUNT         6246 a         6811 c         6429 g         6328 t
ORIGIN      
        1 gcgcgtccga ttggttcacc ggatgcacct taagtacgtt tccttatgtt ataagaacag
       61 aaggatcagc tgtaaaacag caatgatgat tttgatgacc cgtttaatca ggtatcggca
      121 atcagtcatt cgtttttcct taaacaagga atgcagccgt tcagattagc ccttacatct
      181 ccccaaaact gaacgtgcga gttattggaa tcgagtgctg caatccacac cagagctttg
      241 acgacaccac tcgtttcaat gggggaattc agtggcatgg tgtaaagcac agcaaaattg
      301 ccaataacga aaccgatttt aatgtactta tcaaaaagcg gtcacgttaa tcttttcatc
      361 aatgtcgatt tcttcgtaaa ggctcgcgtt ttttatgtca gagattgcaa gttgcttgta
      421 caggtcaacg aaattgatcc ataatcattt tcgttggaat cgtgtgctgc acaaaattaa
      481 agttaaaaag taaaaccccc gttccttacc agttcggggg ttttacttta taaagagaac
      541 ggtattattt taactttcaa taattaccgt ggcacaagca tagtggcgct catcggcaag
      601 cgttacatgc atatttacaa cgccgagctt ttccgccagt tttaatgcct caccccatag
      661 ccgtagccgt ggtttgccga gctcatcatt gaatacttca aactgattaa acgccagacc
      721 attgcggatc cctgtgccaa atgcttttgc cgccgcttct ttcacagcaa aacgcttcgc
      781 cagaaaacgc actggctggt ggtgcgtttt ccagatttcc cattcgttat cgcttaatac
      841 gcggcgtgcc aggcgctcac cggatcgggc gatcaccgct tctatgcgag cgatctctac
      901 gatatccgta cctaaaccta atattgccat tagccacgcg cttccagcat cagacgcttc
      961 atttctgcca ccgcatcttt cagtccggtc atcactgcac gaccaataat ggcatgaccg
     1021 atattcagtt catgcatctc agggatggcg gcaatggctt tcacgttgtg ataggtcaga
     1081 ccgtgtccgg cgttaacttt cagaccgaga ctagtggcaa aggtcgcggc tttggcgata
     1141 cgcgccaact cttgcgcctg ttcggcgtca gttttggcat cagcatagca accggtgtgg
     1201 atctcgataa acggtgcgcc aacctctgcc gcagctttga tctgatcttc atcggcgtcg
     1261 ataaacagag aaaccaggat cccggcctcc gccagacgtt tgcaggcatc gcgcattttg
     1321 tcacgctgcc ctgcgacgtc caggccgcct tcggttgtga cttcctgacg cttttccggt
     1381 accaggcagc aaaaatgtgg cttcgtctca acagcgatcg ccagcatctc ttcggtcacc
     1441 gccatctcca gattcatgcg ggtatccagc gtctggcgca ggatgcgcac gtcgcggtcg
     1501 gtaatgtgac ggcgatcttc acgtaaatgc acggtaatgc cgtccgctcc cgcctgctca
     1561 gcaataaacg ccgcctgcac tggatccggg taagcagtac cgcgtgcatt acgcagcgtt
     1621 gcaatatggt caatgttgac gcctaacagt aattcagcca tgacaatcct catcattcat
     1681 aatgtgtttt caccgttcgc ttaggcataa actgccggaa cagttccctg ctctttaatg
     1741 gtttaccgcc aagatacggc ttaagcgcca tgcgggtaaa gcgtttcgcg gcgcgcagtg
     1801 tgtctgcgtc aggaaattcc cgcgcgttta acgcttttaa ctgccttccg gtgaacgttt
     1861 tattgtcgat aacgacgctt gcgataaacc ctttttcttc gcgataacga tacgtcatgg
     1921 tgtcatctac cggctcgccg ctacccgcac aatgggtaaa attgacgcca tagcccagat
     1981 gcccgagcag tgccagttca aagcggcgca acgcgggttc tggcgtacca gtgaccccgg
     2041 caagagactg aatgcagtgc aagtaatcga aaaagagttc agagaagcgc gtctcgtatt
     2101 ccagtacgcg ggagagaagt tcgttgatgt acagaccgct gtaaagcgtg ataccgctta
     2161 atggcagcgc cagcgagaca gcttcagcac tgcgcagcgt tttgacttcg ccacgcccgc
     2221 caaaacgtag caagagaggg gtgaaaggct gtaatgcacc tttcagagta gagcgtttag
     2281 agcgtgcgcc tttggcaacc agacgcacgc gacccgattc ctccgtgaag acgtccagca
     2341 tcaggctggt ttcgctccac gggcgactat gcaggacaaa tgcgcgctgc cagccttcca
     2401 tcggagttac tcttaaagat cgtcaacata accgagactg cgcagtgcgc gttcgtcgtc
     2461 ggcccaaccg gatttcactt ttacccacag ctcaaggtga acaggtgctt cgaacatttc
     2521 ctgcatgtct ttacgcgctt caatcccgat ggttttgatc ttggcccctt tgttgccaat
     2581 gaccatcttc ttctgccctt cacgctcaac gagaatcaaa ccgttgatgt cataaccgcc
     2641 gcgttcgtta gagacgaaac gctcgatctc cacggtcacg gagtacggca gttcagcgcc
     2701 gagaaaacgc atcagttttt cgcggataat ttcagacgcc ataaaacgct gtgagcgatc
     2761 ggtgatgtaa tcttccggga agtgatgggt cgcttcaggc agatgcttac gcacgattgc
     2821 cgcgatagtg tcgacattca gcccggtttc ggcagagatc ggcacgatat cgaggaagtt
     2881 catctggctt gccaggaact gtaagtgtgg cagcagatcg gctttttcct gcacgttgtc
     2941 cactttgttc accgcgagga ttaccggcgc tttgccatcg cgcagtttgt tgagcaccat
     3001 ttcatcgtcc ggcgtccagc gggtgccttc tacgacaaaa atcaccagct caacgtcgcc
     3061 gatagagctg ctcgccgctt tgttcatcag gcggttaatg gcgcgttttt cttccatatg
     3121 caggcctggg gtatcaacgt agatcgcctg atacgcgcct tcagtatgga tccccacaat
     3181 gcggtgacga gttgtttgcg ccttgcggga ggtgatggag atcttctgcc ccagcagttt
     3241 gttcaataat gtggatttgc caacgttcgg acgtccgacg atagcaataa atccgcagta
     3301 acttttatcg atgctcattc cagctccagt tttttcaacg cctgttcggc ggcagcctgc
     3361 tcagccttac gacggcttga acctgtgcca accaccggtt cactcaggcc gctgacctgg
     3421 cagtggatag taaattcctg atcgtgcgct tcgccacgta cctggactac cagataagtt
     3481 ggcagcggca gatggcgacc ctgcaaatat tcctgcagac gcgttttcgg atctttttgt
     3541 ttatcgcctg ggctaatttc gtccagacga gtttgatacc agttgaggat taatttctcg
     3601 acggtttgaa tatcactgtc gaggaatacg ccaccaatta atgcttcgac ggtgtcggcg
     3661 agaattgact cacgacgaaa tccaccgctt ttaagttcac ctggccctaa acgtaagcac
     3721 tcacctaact caaattcgcg cgccagttca gccagcgtat taccacggac cagcgtggcg
     3781 cgcatccggc tcatatcgcc ttcatccaca cgagggaaac ggtgataaag cgcattggct
     3841 ataacgtagc tcagaataga gtcacctaaa aattctaaac gctcgttatg tttactgctg
     3901 gcactacgat gagttaatgc ctgctgcaac agttcctgat gattaaaagt gtagcccagc
     3961 ttccgttgaa gccgattaat tacgatgggg ttcatgcgat accaataaat aaatgcgtca
     4021 acaattcagc acacgaaaca gacctgatat acatggctcg gctaactgct tcactgcagt
     4081 ttcagtttac cggtatatgg ggaccaacgc tgtttcgtgt gccgtggcaa cctggaggtg
     4141 ccaaccttaa acttcggggg aatattctat acacaacgac gggggatgtc gttagccacg
     4201 ggagatttat ctcataaata attcacgttg tcgccataac ggcgacaacg tgaatgaaga
     4261 tggctattaa tggataccgc caatgcgact taagcgcaca ccagtcggcc attcgccttc
     4321 ttgcttatcg aagctcatcc agatagccgt tgcccgaccg accagattcg cttcaggcac
     4381 aaaaccccag taacgactgt ccgcgctgtt gtcgcggttg tcgcccatca tgaagtattg
     4441 tcccggcgga acaatccagg ttgccagttg ttgccctggc tgctggtaat acatccccac
     4501 ctggtcctgc gcaatcggca ctgtcagaat gcggtgcgtc acatcaccca atgtctcttt
     4561 acgctcggaa agacgaattc cattttcttt ggtttcgttt ttcggcactt caaagaatcc
     4621 gctggtcgct tccccaccat tacggcgtga gaaggtctga acgaaatcgc tcggttccac
     4681 gttggagtaa gtgaccggta gcgcgttttc acacgcctgg ccggaactgc atcccggttg
     4741 aatcgtcagc tcttttgaga ccggatcata agtgacttta tcgcccggca aacccaccgc
     4801 gcgcttgatg taatcaagct ttggatcttc cggatattta aagaccacga tatcgccgcg
     4861 tttcggatga ccggtttcga tcagcgtttt ctggtagata ggatctttaa tgccataagc
     4921 aaacttctct accagaataa aatcaccgat taacagagtc ggcatcatcg aacctgacgg
     4981 gatctggaac ggttcataaa taaacgaacg cacaatcaat acgatagcca gtaccggaaa
     5041 aacagaagca ccggtttcca gccagccagg cttcggcgca acctttttca gcgttgcttt
     5101 atccagtgag tcaccggcag ccgcctgcgc cgctgcctga cgttcccgcc gtttaggtgc
     5161 gaaaaagaat ttatccacgc accataaaat gcccgtcacc agtgtggcaa tcaccagaat
     5221 cagggcaaac atattcgcca tgccaactcc taagggttat ttgttgtctt tgccgacgtg
     5281 cagaatggcg aggaacgctt cctgcggcag ctcgacgtta ccgatctgct tcatgcgttt
     5341 cttaccttct ttctgcttct gcagcagctt tttcttacga ctgatatcgc cgccataaca
     5401 tttagccagt acgtttttac gcagctgttt cacggttgag cgcgcaatga tgtgcgtacc
     5461 aatcgctgcc tgaatggcga tatcaaactg ctggcgcggg atcagatctt tcatcttctc
     5521 caccagctcg cgaccgcggt tttgcgaatt accacggtgg gtgatcagcg ccaacgcatc
     5581 aacacgttca ccgttgatta atacgtctac acgtaccatg tcggacgcct ggaagcgttt
     5641 gaagttgtaa tccagagacg cataaccacg cgaggtagat ttcaggcgat cgaagaagtc
     5701 gagcaccact tccgccatcg ggatctcgta cgtcagcgcc acctgattac cgtggtaaac
     5761 catattggtc tgcacgccgc gtttttctac gcacagcgta ataacgttgc cgagatatgc
     5821 ctgcggtagc agcatgtgac actctgcaat cggctcgcgc agttcgtaaa tgttatttac
     5881 cgctggcagc ttggatggac tgtcgacgta gataacttct cttgacgtgg tttcaacttc
     5941 atacactaca gtcggcgcag tggtgatcag atccagatcg tattcacgtt ccagacgttc
     6001 ctggatgatc tccatgtgca gcaggccaag gaagccgcag cggaaaccaa agcccaacgc
     6061 gctggagctt tccggctcat agaacagtga ggcatcgttc aggctgagtt tacccagcgc
     6121 gtcacggaag gcttcatagt cgtcggaact taccgggaac agaccagcat atacctgagg
     6181 tttgactttc ttaaagccag gcagcgcctt ttctgccgga ttacgcgcca gtgttaaggt
     6241 atcgcctact ggagcgccgt ggatatcttt aatcgcacat accagccagc ctacttcgcc
     6301 acatttcagt tcagtacggt caacctgctt cggcgtgaag atgcccagac gatcggcgtt
     6361 ataggtctgc ccggtgctca tgactttcac tttgtcgccc ttacgcaggg tgccgttttt
     6421 aatacggata agtgaaacaa cgcccagata gttgtcgaac caggagtcga taattagtgc
     6481 ctgcaacggg ccttccggat cgccttccgg cggcggaatg tcgcgcacaa gacgttcgag
     6541 aacgtcctgc acgccaacgc cggttttcgc tgaacagcgc accgcgtcgg tggcgtcgat
     6601 gccgacgata tcttcaattt cttccgccac gcgttcagga tcggctgccg gcaggtcaat
     6661 cttgttcagt accggcacca cttcgagatc catttccatg gcggtgtagc agtttgccag
     6721 ggtttgcgct tctacgccct gcccggcatc gaccaccagc agtgcgcctt cacaggcagc
     6781 cagcgaacgg gaaacctcat aggagaagtc tacgtggccc ggggtgtcga taaagttaag
     6841 ctgataggtt tcgccatcag acgctttgta gtccagcgtc acgctttgcg ctttgatggt
     6901 aatgccacgc tcacgctcaa gatccatgga atcgagaacc tgcgcctcca tttcacggtc
     6961 agacaggcca ccgcagatct ggataatacg gtcagacagc gtcgatttac cgtggtcaat
     7021 gtgagctatg atcgaaaagt tacgtatatt cttcataaag tatgattatt gtgccttaat
     7081 gcccggttaa ccaggctttt agaagtcgct gttctgagct taacgtctgt attaatagaa
     7141 acgccgcatt ctacactaca acattgaggc gaggaaatgt tcataccgta tggattgtgg
     7201 tatctggaaa cgtcctcgca tttgttatgc aaaatgcaac aaagccagtg aaatcactgg
     7261 ctcgcgtctt ccgaagatgt ttcaaatcgc acaaggcctg gcggcaatgc cacgcttaag
     7321 atgatcggtt gccattctgc ccgggcagca aacttacgcg aatagccgcg cgcaatcagg
     7381 aacccgccaa taccgccgag gatcgcacca cataatgctg cgacgtcaga agcaaagagt
     7441 agctgaaata gcgaagcgat gaggaataat cccaccagcg gcgacatata aaccagtaat
     7501 gcggagctaa gaaggctgcc ttcggcgatc cccaattcca ctttttgccc cggcactaac
     7561 ggctcatcac agggtacgac aatggtatgc gtggtttgcg ggccaagttt attcaacacg
     7621 cggctaccgc aaccggcgcg tgaagcgcag ctgctgcatg aggctttaac atcgcaactg
     7681 accagcgcct gcccgttttc ccaggagacg acggtagccc actctttgat cattgcgctg
     7741 ccccgaactt aatattctcg gcaatgcgtt tcgccgtttg cggcggcagt tcaccaacaa
     7801 tggtgatttc ggcgttatca cgtacgcttg tactgacggt tctgcgcccg gtgcgcaaca
     7861 tctgatcggt gctcgatggc gtagcgcggt taacgtttac cgagaagctg aataatccgt
     7921 cggaatagag acgtgattcg ataggcatgt tgtccatcgt cggtagcgga cgtcgactac
     7981 tggaaacttc gctaaaaccc tgtggcagcc aggttggcgt ccagctgaat ttagcttttt
     8041 caccgacagg aacagaaagc aacggcggca aatttgcctt cgccagcgtc tgcatactgc
     8101 tgccgatatc ctgattgacg ttaaaagcaa tcacgcgaaa ttgttccagc gtttcaccat
     8161 cgcgatcaag gagatcaacc cgcatcggta atttcgattc ggtgtccatc cacacgatgt
     8221 agctgtagcg tgtaccatct cgggcaacca cgcgaatgac ttcgcaaaga cgatcagcaa
     8281 tacgcgtgcg cccgacggag ataaagtcgt agtaaggaga aaggcgtttg aaatcggtat
     8341 agatgagcga tggcagagaa tcaacaatgt aatcgccatt aagcgtgaac ggttcaagtc
     8401 ccggttcaaa atagctgatt tcattgccgc gctgtaccac ttcccggcgc gggccatcca
     8461 tttgcaacaa ttgtgcaaga ggacggttat cgaggcgtgc atgtcgataa cgcagagact
     8521 caacaccctg tttattgatg ctgatgaatg acagctcgta attcagtgac tgactggcca
     8581 ggttcatctg ctgtaataac gccccggacg cgggagtggc cgaggcgtta gcagagaata
     8641 acaggctacc tgtcactaat gacatggcaa accaaagttg cttcattact gcgattgagt
     8701 tcctaaagtt tgaattcctg gcacctgtac agcggcttgc tgagtttgtg cctgctcaaa
     8761 ctgaagctgt tcagagtgga gtcggcgttg cagttcgtaa tcctgcaaca ttgcattaat
     8821 gcgacgacgc tgctcctgta cctgctgctg ttgaccatta tttgcggtcg cttcagaagg
     8881 tactcccaga ctaaccgggc tggctttacc catcatcggc agtgtattaa atactggcgt
     8941 ttcgggctgc tgggacgttt cagattgccc attatagtgc tggacgccaa cgataactgc
     9001 aagcgatacg catgctgcta cgcccatttg ggtaagctgt gccgcccacg gacgtacttt
     9061 ctgccagaat ggcattttct gccattgatg cggcgcaggc tgggcttccg ggatcaatgt
     9121 cgccggttga cgtactggct cttcttcaat ggcggccatc acgcgtgaag agatatcgaa
     9181 atggagcacc tcgggagtat caccccgcat tgagtcacgg atcaagtgat agctttccca
     9241 ggttttctgc atttctgggt tatgagccag ttcgttaagc agctcactat ccagcgtttc
     9301 gccatccatt aaagcggaaa gttgttcttt ctgcatgcct aataccctta tccagtatcc
     9361 cgctatcgtc aacgcctgat aagcggttga actttgttat caatagcttc cctcgctcgg
     9421 aagatacgtg aacgcaccgt acctaccgga caatccatga tagcggctat ctcttcatag
     9481 ctcaggccat ccagctcccg caaggttatt gccatgcgta aatcttccgg gagggactca
     9541 atagttcgga aaactatctg tctcagttct tctgacaaca ttaagttctc agggttcgaa
     9601 atttctttca acgcgccgcc actttcgaag ttttcagctt caatggcatc cacatcactg
     9661 gaaggtggac gacgcccctg agcaaccagg taatttttcg ctgtatttac agcaatccga
     9721 tacagccatg tataaaaagc gctatctccc cggaacgaat ccagcgcacg ataggcttta
     9781 ataaaagctt cttgtaccac atcgggaaca tcacccgacg gcacatagcg ggaaaccaga
     9841 ctcgccactt tatgctgata gcgcactacc agtaagttaa aggctttctg atctcccttc
     9901 tggacccgtt caaccaggac ctggtccgtt aactgctcgc tcatccgagg taaagtctcc
     9961 ccaaaccaaa tttccacgcg ctatcgaaac gccactccat tagctgcaat ttgagcaagc
    10021 aaagggttag agtgtctcgt ttttgtaaag ttccgtaacg catctgtttt tgtttgtcat
    10081 gctgtagacg gatcattatc tatcattata agtctacaga atctgaacat cgcattatct
    10141 gtgtagaaat gcccatttaa ctgcctgaag agtaacccaa cggccttttt atttcaccac
    10201 ctaatcctcc accagccagt aacttctcgt tttctcgccg ccctgaggca gcgtgtttag
    10261 tcactgtaac aaatattaaa atagcaggtg tttattcgca caacatgatg ttatgctgac
    10321 caaaccgtgt ttagtaaatt aaacaaagaa aatgaatact ctccctgaac attcatgtga
    10381 cgtgttgatt atcggtagcg gcgcagccgg actttcactg gcgctgcgcc tggctgacca
    10441 gcatcaggtc atcgttctaa gtaaaggccc ggtaacggaa ggttcaacat tttatgccca
    10501 gggcggtatt gccgccgtgt ttgatgaaac tgacagcatt gactcgcatg tggaagacac
    10561 attgattgcc ggggctggta tttgcgatcg ccatgcagtt gaatttgtcg ccagtaatgc
    10621 acgatcctgt gtgcaatggc taatcgacca gggggtgttg tttgataccc acattcagcc
    10681 gaatggcgaa gaaagctacc atctgactcg tgaaggtgga catagtcacc gtcgtattct
    10741 tcatgccgcc gacgccaccg gtagagaagt agaaaccacg ctggtgggca aggcgcagaa
    10801 ccatccgaat attcgcgtgc tggagcgcag caacgcggtt gatctgattg tttctgacaa
    10861 aattggcctg ccgggcacgc gacgggttgt tggcgcgtgg gtatggaacc gaaataaaga
    10921 aacggttgaa acctgccacg caaaagcagt ggtactggca accggcggtg cttcaaaagt
    10981 ttatcagtac accaccaatc cggatatttc ttctggcgat ggcattgcta tggcgtggcg
    11041 cgcaggctgc cggattgcca atctcgaatt taatcagttc caccctaccg cgctgtatca
    11101 cccacaggca cgcaatttcc tgttaacgga agcactgcgc ggcgaaggcg cttatctcaa
    11161 gcgcccggat ggcacgcgtt ttatgcccga ttttgatgag cgtggcgaac tggctccgcg
    11221 cgatattgtc gcccgcgcca ttgaccatga gatgaaacgc ctcggcgcgg attgtatgtt
    11281 cctcgacatc agccataagc ccgccgattt tattcgccag catttcccga tgatttatga
    11341 aaaattgctc gggctgggga tagatctcac gaaagaaccc gtgccgattg tgcctgctgc
    11401 acattatacc tgcggtggtg taatggttga tgatcatggg cgtacggacg tcgagggttt
    11461 gtatgccatt ggcgaagtga gttataccgg cttacacggc gctaaccgca tggcctcgaa
    11521 ttcattgctg gagtgtctgg tctatggctg gtcggcggca gaagatatca gcagacgtat
    11581 accttatgcc cacggcgtca gtacgttacc gccgtgggat gaaagccgcg ttgagaaccc
    11641 tgacgaacgg gtagtaattc agcataactg gcacgagcta cgtctgttta tgtgggatta
    11701 cgttggcatt gtgcgcacaa cgaagcgcct ggaacgcgcc ctgcggcgga taaccatgct
    11761 ccaacaagaa atagacgaat attacgccca tttccgcgtc tcaaataatt tgctggagct
    11821 gcgtaatctg gtacaggttg ccgagttgat tgttcgctgt gcgatgatgc gcaaggagag
    11881 ccgtggcctg catttcacgc tggattatcc ggaactgctc acgcattccg gtccatcgat
    11941 cctttctgcc ggcaatcatt acataaacag ataaaaagcc tgggtcagcg ccgtatacgc
    12001 ttcggaatag ttctggtctg gcccacgaat gactaagcga tcgctaaagc attctcccgc
    12061 ctgcggggag aatgccagca gcacccgatg cggcagtcgc gcttcgtttt ccgccacatc
    12121 cgtccgcaaa cgtaaatgcc agcccatact taatgccagt tcagtaaaac cattaccaat
    12181 ctgctctggc agcaccacgc agaaaaatcc ctcttcggta atgcactccg ccgcacaagt
    12241 cagcaacgat gggtgatcaa gcgtagtggt atagcgagcc tgttcccgtt gaggtgtcgc
    12301 gcactctact ccctgctgat agtaaggtgg gttgctgatg attaaatcga agcgtactgt
    12361 ctgctgtgtg atccactgca gaatatccgc cgtatggacg ttaatccgct ctgcccacgg
    12421 ggactggttg atattttcct gcgcttgcgc ggcagcttca ctttccagtt caactgcatc
    12481 aatcatcacg ctgtcatctg ttcgctgcgc cagcattaat gccaacaacc cgctacccgc
    12541 gccgatatca aggcaacgtt ttaccccagc cacaggtgcc catgcgccca gcaaaatgcc
    12601 atccgttccc actttcatcg cacagcgatc gtgagcaaca aaaaactgtt taaaagtaaa
    12661 tccattacga cgaaacacgg atgtagactg tgacatgaaa ataaaacctt gcaggaaaaa
    12721 cggcgatagc accgggtgag aacaataccc gagaagcgat atccatacaa acagatgaag
    12781 attgcagccg taacgtctat aatcagcgcc ccacacagag gtagaacatg actgtaacga
    12841 ctttttccga acttgaactc gacgaaagcc tgctggaagc cctccaggat aaaggtttca
    12901 ctcgcccgac cgccattcag gctgccgcca ttccgcctgc gctcgatggc cgtgatgtac
    12961 tcggttctgc gccgacaggc accggtaaaa cggcggcgta tctgctgcca gcgttgcagc
    13021 acctgctcga tttcccacgt aagaaatccg gtccgccgcg tattttgatc ctcaccccga
    13081 ctcgcgaact ggcgatgcag gtggccgatc atgcccgcga actggcgaaa catacgcatc
    13141 tggatattgc caccatcacc ggcggcgtgg cctatatgaa ccacgcagaa gtgttcagcg
    13201 aaaaccagga cattgtggtc gccacgaccg gacgtctgct gcaatacata aaagaagaga
    13261 acttcgattg ccgcgcggtt gaaacgctga tcctcgacga agcagaccgt atgctggata
    13321 tgggcttcgc ccaagatatc gaacatattg ctggcgaaac gcgctggcgt aaacagaccc
    13381 tgctcttttc ggcaacgctg gaaggcgatg cgattcagga ctttgccgag cgtctgttgg
    13441 aagatccggt ggaagtttct gccaatccgt ccacccgtga gcgcaaaaaa attcatcagt
    13501 ggtattaccg cgccgatgat cttgagcata aaaccgcgtt gctggtgcat ctgttaaaac
    13561 agccggaagc gacccgctca attgtgtttg tgcgtaagcg tgagcgtgtg catgagctgg
    13621 caaactggct gcgcgaagca ggcatcaaca actgctatct cgaaggtgag atggtacaag
    13681 gcaagcgtaa cgaagcgatt aaacgcttga ccgaaggccg cgtaaacgta ctggttgcta
    13741 ccgatgttgc cgcgcgcggt atcgacattc ctgacgtcag ccacgtcttt aacttcgata
    13801 tgccgcgcag tggcgatact tatttgcacc gtatcggacg taccgcgcgc gccggtcgta
    13861 aaggcaccgc gatttcgctg gtggaagccc atgaccatct gctgctgggc aaagttggtc
    13921 gctatattga agaaccaatt aaagctcgcg taattgacga gttacgcccg aaaacgcgtg
    13981 cgccaagcga gaagcagacc ggcaagccat cgaagaaagt actggcaaaa cgcgccgaga
    14041 agaaaaaagc taaagagaaa gagaagccgc gggtgaaaaa acgccatcgc gacaccaaaa
    14101 atattggtaa gcgccgtaaa ccaagcggaa cgggcgtgcc accgcaaacg acagaagagt
    14161 aatctcaatg ccgggtttaa gcccggcatt gtttttatcc cggcaaaaaa cactcttcaa
    14221 cacactgaat aaaagtgtgc atcgccgggc taaccgcttt tccggcatgg tgagcgcaca
    14281 ttgccgtaat ggtctgcgac tgttcgccaa aaggcaattc aattaattct ccgcactcca
    14341 actcttttac caccgcaaaa cgcggcaggt aactgactcc gatatttgcc gcgacacaac
    14401 gcttgatgct ttcgatactt ataagctcaa tggtgttttc caccgtgatc cgccgctggc
    14461 gcagcgtgct ctcaaatatc tgccggaaga cacattgtgg ttcgttgata ataaagctac
    14521 aggcgttatg tcttcccggc tcagtaaaat cgacatctgc aatttgcggt gaagccacca
    14581 gcgccagcgg ttgttcaccc aactctcgtc ggttcagagc atcatcattc cctacgcgat
    14641 aaaaaacccc gacatcggcc tcatcattca gtagcgcatc acggatcacg taacagttca
    14701 gcgactgcaa cgataaacgc acttttggag cgcgttgccg aaaacgctgc aacacctgcg
    14761 gcattcgata agagagcaac gtttcgcctg aaacaacgcg tagttccccg tccggatccg
    14821 actctttttt cgcagcctca cgaagcgtat ccatcactcg ggtaagttca tagatgtgcg
    14881 gcagcaactt ttttccttcg cgggtaaggc acattcgtcg accaattttc tcaaacaatt
    14941 gtaccgagaa ctcctgctca agctgctgaa tatgaaaagt caccgtcgat tgtgtacagc
    15001 acaatttttg cgaagctcgc aaaaaggaac cctcttccac cacggtttta agcgtaataa
    15061 aacggcgcag atccataacc ccaaacctat cgaaaatatc gaatttagaa tgtaaaaaca
    15121 ttcatttttt taaatgttcc gtgtcgggta ctgtctacca aaacagagga gataacaagt
    15181 gacaccgacc cttttaagtg ctttttggac ttacaccctg attaccgcca tgacgccagg
    15241 accgaacaat attctcgccc ttagctctgc tacgtcgcat ggatttcgtc aaagtacccg
    15301 tgtgctggca gggatgagtc tgggattttt gattgtgatg ttactgtgtg cgggcatttc
    15361 attttcactg gcagtgattg acccggcagc ggtacacctt ttgagttggg cgggggcggc
    15421 atatattgtc tggctggcgt ggaaaatcgc caccagccca acaaaggaag acggacttca
    15481 ggcaaaacca atcagctttt gggccagctt tgctttgcag tttgtgaacg tcaaaatcat
    15541 tttgtacggt gttacggcac tgtcgacgtt tgttctgccg caaacacagg cgctaagctg
    15601 ggtagttggc gtcagcgttt tgctggcgat gattgggacg tttggcaatg tgtgctgggc
    15661 gctggcgggg catctgtttc agcgattgtt tcgccagtat ggtcgccagt taaatatcgt
    15721 gcttgccctg ttgctggtct attgcgcggt acgcattttc tattaacgaa aaaaagcgga
    15781 agaggtcgcc ctcttccgct tagtaacttg ctacttaagc cttacaggct ttcagtaaag
    15841 gtacgagcga taacgtcgcg ctgctgttcc ggagtcagag agttaaagcg aactgcataa
    15901 ccggatacac ggatggtcag ctgcggatat ttttccggat gcttaactgc atcttccaga
    15961 gtttcacgac gcagaacgtt aacgttcagg tgttgaccac cttcaacgcg aacttctggt
    16021 ttcacttcta ctggaacttc acggtattca atgtcaccca gtttgcttac tgcaaccact
    16081 tcgtcttctg caaaacctgc tttcgcaacg atgcaacgcg cttcgccttt ttcgctgtcc
    16141 agcagccaga aagagttcag cagatcgtcg ttagcggctt tagtaatctg gatacctgta
    16201 atcatgtgat gcctccccgg caaaattatt tgatttgttc agcctgtcgc ggccaattgg
    16261 taaaaccatt gttgcttgag tgtatatata ctcctcaaac acccttgatt ctttgattta
    16321 aatcaacaaa aaccacatat caagtgtggt cacaaatgga ttttattgtt ttacatcaac
    16381 ttatgcagat gtgaaatttt accaatttac atttttttgc atgtcttttt gtctataaaa
    16441 tgagcatgat tttgttctgt agaaagaagc agttaagcta ggcggattaa agattcgcag
    16501 gagagcgaga tggctaacga attaacctgg catgatgtgc tggctgaaga gaagcagcaa
    16561 ccctattttc ttaataccct tcagaccgtc gccagcgagc ggcagtccgg cgtcactatc
    16621 tatccaccac aaaaagatgt ctttaacgcg ttccgcttta cggagttggg tgacgttaaa
    16681 gtggtgattc tcggtcagga tccttatcac ggaccaggac aggcgcatgg tctggcattt
    16741 tccgttcgcc ccggcattgc gacaccaccg tcactgctga atatgtataa agagctggaa
    16801 aatactattc ccggtttcac ccgccctaat catggttatc ttgaaagctg ggcgcgtcag
    16861 ggtgttctgc tgctcaatac cgtcttgacg gttcgcgctg gtcaggcgca ttcccacgcc
    16921 agcctcggct gggagacgtt tactgataaa gttatcagcc tcataaatca gcatcgcgaa
    16981 ggcgtggtgt ttttgttgtg gggatcacat gcgcaaaaga aaggggcgat tatagataag
    17041 caacgccatc atgtactgaa agcaccgcat ccgtcgccgc tttcggcgca tcgtggattc
    17101 tttggctgca accattttgt gctggcaaat cagtggctgg aacaacgtgg cgagacgccg
    17161 attgactgga tgccagtatt accggcagag agtgagtaaa tttgcgggaa aatgccggat
    17221 ggcggagttg ccacccggct gatttatcag gctttattct gacgccacca ttcgccaaga
    17281 agaacgccgg ttgcgacaga gatattcagc ccggcaacgt tgccagtacc atcaatcttc
    17341 acgcgcagat cgttcggatc gcgtgcggca tccggtaacc cttcatattc ctgacccagc
    17401 accagtacca ttttcgctgg cagactggtt ttgaacagcg gtttaccctg ctcgctggaa
    17461 gttgtcacta cggtgtaacc cgcctgacgg aaatcatcca gcatgttaac aatgttgtcg
    17521 ccagtaatcg gctgaacgtg ctctgcgccg ccttctgcgg tacggatagc cgccccagac
    17581 tccagcagtg ctgcatcctg caccacaaca cctttcacgc cgaagtgcgc acagctacgc
    17641 atcatgccgc ccaggttatg cgggttagat tcgttttcca gggccagtac acaatcctgc
    17701 gcgcctgcct ggcttaccca ctgctgcacg gttgtaccgt tacgcttttt gatcaggaag
    17761 caaacgccgc cgtgatgttc cgtgcctgac gcttttgtca gttccgcttc atccaccaca
    17821 tggtacgctt tgcggtttgc cgccatccag cgcaaggctt ctttaaaacg tggcgttacg
    17881 ctctggataa accaggcgcg aacaatcgct tccggacggc tctggaacag tgcctgacag
    17941 gcgttttcgc cgtagacgcg ggtttcttcc gcacgctgac gacgcaaaac ttccggatca
    18001 ataaaacttt taccactgat gccaccgtga tcggcctttt ccggcgtctc atcacccggc
    18061 gcgcgggaaa ccgtgcgcca cggcgaatct tcccacttgc ggtcacggtg ctgactctgt
    18121 ttgtcatcgc gggcggggcg acggccaccg tcagcacgag attttcctgg acgcccgccc
    18181 cctttcccgg tacgcgggtt gtgggtacgt ttatcagaat catcatcact gcggacatac
    18241 atcactttga ccttgccgct tttacctttc atttcatcgt tcatgctttt ctccaccagc
    18301 gctgcgcgaa gcgcgcagat tacccgaagt gcgcgcggtt cgccatgatt tcgttccaaa
    18361 gcctgtgtct atcataccca ttgaataaaa cagattgttg tctggaacaa tgtccccgat
    18421 aatatgtaac atattagaaa cataccggcg tcattgccga taagtctcct tactcatccc
    18481 gaggttagtt atgaataccg tttgtaccca ttgtcaggcc atcaatcgca ttcccgacga
    18541 tcggatcgaa gatgcggcaa aatgcggacg ctgcggtcac gacttgtttg acggcgaggt
    18601 gattaatgcg accggtgaaa cgctcgacaa attgctgaaa gatgatctac ctgtggtgat
    18661 cgacttctgg gcaccgtggt gcggcccctg ccgtaatttc gcaccaattt ttgaagatgt
    18721 cgcgcaagag cgtagcggta aagggcgctt tgtgaaagtg aataccgaag ctgaacgtga
    18781 attgagcagt cgctttggaa ttcgtagtat accgacgatc atgattttca aaaacggtca
    18841 ggttgtcgac atgcttaatg gcgcagtacc gaaagcgccg ttcgacagct ggctgaacga
    18901 atctctttaa tcttaccggg gcgcatcttg tgccccgttt tctcctctgc gacaatggcg
    18961 ttttttcgac gctctcttat gaccgaaaac gctgttctcc agttacgcgc cgagcgtatt
    19021 gcgcgcgcaa cacgtccttt tcttgcccgc ggtaatcgcg ttcgtcgctg ccaacgctgt
    19081 cttttgccag agaaattatg tctctgttcg acaattaccc cagcacaagc aaaaagtcgc
    19141 ttctgtttgc tgatgttcga caccgagcca atgaagccca gtaataccgg gcgtctgatt
    19201 gctgatattt tgcctgatac cgttgcgttt caatggtcgc gtacagaacc ctcgcaagat
    19261 ttgctggagc tggtgcaaaa cccggactat caaccgatgg tggtttttcc cgcctcgtat
    19321 gctgatgagc aacgggaagt gatcttcaca ccacctgccg gtaatcctcc gctatttatc
    19381 atgctcgatg gcacctggcc agaagctcgc aagatgtttc gtaaaagtcc gtatctggat
    19441 aatcttcccg tcatttccgt cgatctttct cggctttctg cctatcgcct gcgtgaagcc
    19501 caggctgaag gccaatattg tactgccgag gtagccatcg cactgttaga tatggccggc
    19561 gataccaggg cggcgacagg gttaggcgag cattttaccc gctttaaaac acgctatctg
    19621 gcaggaaaaa cgcaacatcc gggaagcatc acagcagaac agttagaaag cgtttaaaat
    19681 cattcggtca cttctgcggg agaccggtat gagtcagcga ggactggaag cactactgcg
    19741 accaaaatcg attgcggtaa ttggcgcatc gatgaaaccc aatcgcgcag gttacctgat
    19801 gatgcgtaac ctgctggcgg gaggctttaa cggaccggta ctcccggtga cgccagcctg
    19861 gaaagcggtg ttgggtgtgt tggcctggcc ggatattgcc agcttgccct ttacacccga
    19921 ccttgcggtt ttatgtacca atgccagccg taatcttgct cttctggaag agctcggcga
    19981 gaaaggctgt aaaacctgca ttattctttc cgccccggca tcgcaacacg aagatctccg
    20041 cgcctgcgcc ctgcgccata acatgcgcct gctaggacca aacagtctgg gcttactggc
    20101 tccctggcaa ggtctgaatg ccagcttttc gcctgtgccg attaaacgcg gcaagctggc
    20161 gtttatttcg caatcggctg ctgtctccaa caccatcctc gactgggcgc aacagcgtga
    20221 gatgggcttt tcctacttta ttgcgctcgg cgacagcctg gatatcgacg ttgatgaatt
    20281 gctcgactat ctggcgcgcg acagtaaaac cagcgccatc ctgctctatc tcgaacagtt
    20341 aagcgacgcg cgacgctttg tttcggcagc ccgtagtgcc tcgcgcaata aaccgatttt
    20401 ggtgattaaa agcggacgta gcccggcggc tcaacgtctg ttgaacacca cggcaggaat
    20461 ggatccggcg tgggatgcag ctattcagcg tgccggtttg ttgcgggtac aggacaccca
    20521 cgaactgttt tcggcggtgg aaacccttag ccatatgcgc ccgctgcgtg gcgaccggtt
    20581 gatgattatc agcaacggtg ctgcgcctgc cgcgctggcg ctggatgcct tatggtcacg
    20641 caatggcaag ctggcaacgc taagcgaaga aacctgccag aaactgcgcg atgcactgcc
    20701 agggcatgtg gcaatctcta acccgctcga tctgcgcgat gacgccagca gcgagcatta
    20761 tgtcaaaacg ctggacattc tgctccacag ccaggatttt gatgcgctga tggttattca
    20821 ttcgcccagc gccgctgctc ccgcaacaga aagcgcgcaa gtattaattg aagcggtaaa
    20881 gcatcatccc cgcagcaaat atgtctcttt gctgacgaac tggtgcggcg agcactcctc
    20941 gcaagaggca cgacgtttat tcagcgaagc cgggctgccg acctaccgta ctccggaagg
    21001 aaccatcact gcttttatgc atatggtcga gtaccggcgt aatcagaagc aactacgcga
    21061 aacgccggcg ttgcccagca atctgacttc caataccgca gaagcgcatc ttctgttgca
    21121 acaggcgatt gccgaagggg ctacgtcgct cgatacccat gaagttcagc ccatcctgca
    21181 agcgtatggt atgaatactc tccctacctg gattgccagt gatagcaccg aagcggtaca
    21241 tattgccgaa cagattggct atccggtggc actgaaattg cgctcgccgg atattccaca
    21301 taaatcggaa gttcagggcg tcatgctcta cctacgtaca gccaatgaag tccagcaagc
    21361 ggcaaacgct attttcgatc gcgtaaaaat ggcctggccg caggcgcggg tccacggcct
    21421 gttggtgcaa agtatggcta accgtgctgg cgctcaggag ttgcgggttg tggttgagca
    21481 cgatccggtt ttcgggccgt tgatcatgct gggtgaaggc ggtgtggagt ggcgtcctga
    21541 agatcaagcc gtcgtcgcac tgccgccgct gaacatgaac ctggcccgct atctggttat
    21601 tcaggggatc aaaagtaaaa agattcgtgc gcgcagtgcg ctacgcccat tggatgttgc
    21661 aggcttgagc cagcttctgg tgcaggtttc caacttgatt gtcgattgcc cggaaattca
    21721 gcgtctggat attcatcctt tgctggcttc tggcagtgaa tttaccgcgc tggatgtcac
    21781 gctggatatc gcgccgtttg aaggtgataa cgagagccga ctggctgtgc gcccttatcc
    21841 gcatcagctg gaagaatggg tagaattgaa aaacggtgaa cgctgcttgt tccgcccgat
    21901 tttgccagaa gatgagccac agcttcagca attcatttcg cgagtcacca aagaagatct
    21961 ttattaccgc tactttagcg agatcaacga atttacccat gaagatttag ccaacatgac
    22021 gcagatcgac tacgatcggg aaatggcatt tgtagcggta cgacgaattg atcaaacgga
    22081 agagatcctc ggcgtcacac gtgcgatctc cgaccctgat aacatcgatg ccgaatttgc
    22141 cgtgctggtt cgctcggatc tcaaagggtt aggcttaggt cgacgcttaa tggaaaagtt
    22201 gattacctat acgcgagatc acggactaca acgtctgaat ggtattacga tgccaaacaa
    22261 tcgtggcatg gtggcgctgg cccgcaagct cgggtttaac gttgatatcc agctcgaaga
    22321 ggggatcgtt gggcttacgc taagtcttgc ccagcgcgag gaatcatgag taaggtactg
    22381 gaaatgttga ccacttaatc gggactggtg ttattattgc ccgcttatgt cgtctgcatt
    22441 gcacagagga cccttcaatg aacagagaag aaatgcactg tgatgttgtc aaaatttaag
    22501 cgtaataaac atcaacaaca ccttgcccaa ctacccaaga tttctcaatc agttgatgat
    22561 gtcgatttct tttacgctcc cgccgacttc cgggagacgt tgctggaaaa aatagccagc
    22621 gcgaagcagc gcatttgcat tgttgccctg tatctcgaac aggatgacgg tggcaaaggc
    22681 attctgaacg cgttgtatga ggctaaaagg cagcgtccgg aactggatgt gcgggtgctg
    22741 gtcgactggc atcgtgcaca acgtggacgc attggcgctg cggcatctaa cactaacgct
    22801 gactggtact gccgcatggc gcaggaaaat ccgggcgtag atgttccggt ttatggcgtg
    22861 ccaatcaata cccgtgaagc ccttggtgtt ctgcacttta aaggctttat catcgacgat
    22921 agcgtacttt atagcggtgc cagcctgaac gatgtttacc tgcatcagca cgataaatat
    22981 cgctacgacc gttatcatct gatccgtaac cgtaagatgt cagacattat gtttgaatgg
    23041 gttacacaga atattatgaa tggccgcggc gttaatcgtc tggatgatgt taatcggcca
    23101 aaaagcccgg aaatcaagaa cgacattcgc ctgttccgcc aggagctgcg tgatgccgct
    23161 tatcatttcc agggcgatgc cgacaacgat cagctttccg taacgccgct agtggggctg
    23221 gggaaatcga gtctgttgaa caagaccatt ttccatctta tgccttgtgc cgagcagaaa
    23281 ctaaccatct gtacgccata cttcaacctg ccagcaatcc ttgtgcgcaa tattatccag
    23341 ttgctgcgcg aagggaaaaa ggtcgaaatt attgttggtg ataaaaccgc gaacgacttc
    23401 tacattccgg aagatgaacc tttcaagata attggcgcat tgccttatct ctatgagatc
    23461 aatctgcgtc gtttcctgag tcgtttacag tattacgtca acactgacca gttagtagtt
    23521 cggttatgga aagatgacga caacacctat cacctgaaag ggatgtgggt tgatgataag
    23581 tggatgttga tcaccggtaa taacctgaac ccgcgcgcct ggcgtctgga tctggaaaac
    23641 gccattttga tccacgatcc gcaactggag cttgcgccac agcgagagaa agaactggat
    23701 ctgatccgcg agcataccac catcgttaag cactaccgcg atctgcaaag tattgccgat
    23761 tatccggtga aggttcgtaa actcatccgc cgtttgcgcc gtatccgcat cgaccgatta
    23821 attagccgca tcctgtaatc acaaccccgt cctgtacggg gtttgttttt tggaggccac
    23881 gttttgcgta ttctttttgt ctgttcgcta ttgttacttt ctggatgcag ccatatggct
    23941 aacgataact ggagcgggca ggataaagct caacacttta tcgcctcggc gatgctttcc
    24001 gccgccggaa atgaatattc acagcatcag gggatgagcc gggatcgcag tgccatgttt
    24061 ggattgatgt tctctgtcag tctgggggcg tcaaaagagc tttgggatag ccgccctgag
    24121 gggagcggct ggagctggaa ggattttgcc tgggatatcg ccggtgcaag caccggctat
    24181 accgtctggc aactgacccg tcactaaagc cgcatccctt ttcctttacg gtgcaatgtt
    24241 agtgaaacca gaaacgccac cacggccatc aaggtcacat accagaagaa ggctgtttcc
    24301 attcctattg atttcagcga caacgctacg tactccgccg acccaccaaa tatagcattg
    24361 gcgaccgcat atgacagacc aacgcctaat gcgcgaacct gtgccgggaa catctcagcc
    24421 ttcagtattc cactgattga agtataaaaa ctcactatca gcagggcgca catcaccaga
    24481 ccaaaagcgg cataaggcga ggaaacgttc tgcaatgctg agagaatagg aacggtaaaa
    24541 atggttgcca gagaaccgaa acataacatt gaggtacggc gaccaatctt atccgacagc
    24601 gcgccaataa gtggttgaat aagcatgaat acaaacaatg cggcagtcat aatgccactc
    24661 gccacgttgg catgcattcc cgcagtattt accagatact tctgcatata agtggtgaag
    24721 gtatagaaac aaagggagcc cgcagcggta aaaccgagaa ccatgatgaa tgcacggcga
    24781 ttgcgccata atcctttcag agatccagct tcttttaaag cgcgcgtttc ttgttgcgaa
    24841 gtttcatcta actgacgacg taaccacaac gccacaacag ctaacacagc tcctaacgcg
    24901 aaaggaatac gccatcccca ctctctgagt gcagcgtctt ccatggtatg ttgtaaaacc
    24961 acgacaacca gtagggctag cagttgtccg ccgatcaacg tcacatactg aaatgatgcg
    25021 taaaaacctt tgcgcccttc aacggcaact tcactcatat aggtggcgct ggtgccatat
    25081 tctccgccaa cggataatcc ctgaaataaa cgagcgagaa gcaataatgc cggagcccac
    25141 gtacctatag tttcataacc agggaggcag gcgataacca gcgatccgaa acacatcata
    25201 cacaccgata acagcatcga ttttttgcga ccatgtttat cggctatgcg gccaaatagc
    25261 caaccgccta ttgggcgcat caggaatccc gcagcaaaaa cacctgctgt ttgtagtagt
    25321 tgagtcgtcg tgttcccgga agggaagaaa atgtgggcga agtagagtga acagaacgag
    25381 tagacataga aatcgaacca ctcgaccaga ttacctgaag aggccccaac aattgcccaa
    25441 atgcggcgac gagtatcact acttgtcagt ttgctgtctg ccgttacagt actttcagcc
    25501 atgccattat gtctcctgcc gtaatccgat gcttttgtcg gtcgcttttg tttatttcta
    25561 ttggtaaaga aaatgttata catttgttgc atatcattat gcaaccttaa ccacgaattt
    25621 agttagcagg aaaatggtta ttgaggagct taaggataaa tttctggtaa ggaggacacg
    25681 tatggaggtg ggcaagttgg ggaagccgta ttcgctgctg aatctagctt atgtgggagt
    25741 ataagacgcg ccagcgtcgc atccggcatt ttttctgctc caatgcaaaa aggccatcct
    25801 ttcggatggc cttt
//
LOCUS       DADSZD010000058        25591 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.058, whole genome shotgun sequence.
ACCESSION   DADSZD010000058 DADSZD010000000
VERSION     DADSZD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.058"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(29..625)
                     /gene="nrfG"
                     /locus_tag="J1Z28_002097"
     CDS             complement(29..625)
                     /gene="nrfG"
                     /locus_tag="J1Z28_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBA7716932.1"
                     /translation="MKQPQIPVKMLTTLTIFMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(622..1005)
                     /gene="nrfF"
                     /locus_tag="J1Z28_002098"
     CDS             complement(622..1005)
                     /gene="nrfF"
                     /locus_tag="J1Z28_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBA7716933.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(998..2716)
                     /locus_tag="J1Z28_002099"
     CDS             complement(998..2716)
                     /locus_tag="J1Z28_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBA7716934.1"
                     /translation="MDVFLPEVSFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLATLLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFGGTCARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRARLPTLLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(2736..3692)
                     /gene="nrfD"
                     /locus_tag="J1Z28_002100"
     CDS             complement(2736..3692)
                     /gene="nrfD"
                     /locus_tag="J1Z28_002100"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBA7716935.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRVYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(3689..4360)
                     /gene="nrfC"
                     /locus_tag="J1Z28_002101"
     CDS             complement(3689..4360)
                     /gene="nrfC"
                     /locus_tag="J1Z28_002101"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBA7716936.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(4357..4923)
                     /gene="nrfB"
                     /locus_tag="J1Z28_002102"
     CDS             complement(4357..4923)
                     /gene="nrfB"
                     /locus_tag="J1Z28_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBA7716937.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(4968..6404)
                     /gene="nrfA"
                     /locus_tag="J1Z28_002103"
     CDS             complement(4968..6404)
                     /gene="nrfA"
                     /locus_tag="J1Z28_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBA7716938.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6796..8754
                     /gene="acs"
                     /locus_tag="J1Z28_002104"
     CDS             6796..8754
                     /gene="acs"
                     /locus_tag="J1Z28_002104"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBA7716939.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLEMGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            9057..9371
                     /locus_tag="J1Z28_002105"
     CDS             9057..9371
                     /locus_tag="J1Z28_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBA7716940.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            9368..11017
                     /gene="actP"
                     /locus_tag="J1Z28_002106"
     CDS             9368..11017
                     /gene="actP"
                     /locus_tag="J1Z28_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBA7716941.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(11056..11745)
                     /locus_tag="J1Z28_002107"
     CDS             complement(11056..11745)
                     /locus_tag="J1Z28_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBA7716942.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(11738..12148)
                     /locus_tag="J1Z28_002108"
     CDS             complement(11738..12148)
                     /locus_tag="J1Z28_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBA7716943.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            12252..13136
                     /locus_tag="J1Z28_002109"
     CDS             12252..13136
                     /locus_tag="J1Z28_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7716944.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(13172..14821)
                     /locus_tag="J1Z28_002110"
     CDS             complement(13172..14821)
                     /locus_tag="J1Z28_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBA7716945.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(14972..16321)
                     /locus_tag="J1Z28_002111"
     CDS             complement(14972..16321)
                     /locus_tag="J1Z28_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7716946.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(16869..17333)
                     /gene="soxR"
                     /locus_tag="J1Z28_002112"
     CDS             complement(16869..17333)
                     /gene="soxR"
                     /locus_tag="J1Z28_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBA7716947.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            17419..17742
                     /gene="soxS"
                     /locus_tag="J1Z28_002113"
     CDS             17419..17742
                     /gene="soxS"
                     /locus_tag="J1Z28_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBA7716948.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(17778..19331)
                     /gene="pdeC"
                     /locus_tag="J1Z28_002114"
     CDS             complement(17778..19331)
                     /gene="pdeC"
                     /locus_tag="J1Z28_002114"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBA7716949.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNYLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKNQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMG"
     gene            19760..20041
                     /locus_tag="J1Z28_002115"
     CDS             19760..20041
                     /locus_tag="J1Z28_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716950.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(20140..20676)
                     /gene="ssb1"
                     /locus_tag="J1Z28_002116"
     CDS             complement(20140..20676)
                     /gene="ssb1"
                     /locus_tag="J1Z28_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBA7716951.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            20931..23753
                     /gene="uvrA"
                     /locus_tag="J1Z28_002117"
     CDS             20931..23753
                     /gene="uvrA"
                     /locus_tag="J1Z28_002117"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBA7716952.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(23788..24144)
                     /locus_tag="J1Z28_002118"
     CDS             complement(23788..24144)
                     /locus_tag="J1Z28_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBA7716953.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(24148..24564)
                     /locus_tag="J1Z28_002119"
     CDS             complement(24148..24564)
                     /locus_tag="J1Z28_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBA7716954.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGAWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(24675..25388)
                     /gene="aphA"
                     /locus_tag="J1Z28_002120"
     CDS             complement(24675..25388)
                     /gene="aphA"
                     /locus_tag="J1Z28_002120"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBA7716955.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         6334 a         6994 c         6539 g         5724 t
ORIGIN      
        1 caataacata atcttcatca ccagatgatt aatctgatct ccgctgcaac aatttcgcca
       61 tattaatcga ctcgaccagc tgtgtccggt tgatccgggg tgagttgaga tccatcactt
      121 tttgccatag ttcgatggct tgcgcgtaat tcgcctgcat aaacgcatcg gaagccagca
      181 gcatcagagc ggtgatttcg ttagagtcca gcgcgagggc tttgtcgatc attgcgcgag
      241 cctggacggt catatgttgg ctggcctggt aatacagcac cgtcgccagc gccgcataca
      301 gttcagcgtt ctcaccacgt agttgcaacg cctgacggta cgccagcagc gaattgctgt
      361 aatcgttttg ccacagataa tactcgccca gtaacgccca ctgttcgctg ttttgtggat
      421 tagcacggat tttatcctgc aatgcctgaa gctgcgcttc cggggtttgc tggctggcaa
      481 actgatgcag cggatcgcgc tgacgctgat actccgcacg taccgcctgc cattttggac
      541 ttaacagata actgccgaca cagagaaata ccataaaaat cgtgagtgtt gtcagcattt
      601 tcaccggtat ttggggctgt ttcatcgctt cgccctcact cgccagagga tcagtgccat
      661 cagcagtaac aacactactg gcagcgccca caacaccagc gtctgacccg ttaacggtgg
      721 gttatagcga acaaaatctc cgtagcgttc ggtcatccag ccaatgattt cgacttcact
      781 tttcccctct tccaccatgg tgtaaacctg atggcgcatg ctgactgcca ccggcgcatt
      841 ggattccagt aagttctgat tctggcactg cggacaacgt aactggctgg caatatttaa
      901 cgcctgttcc tgttgttgcg gattggcgaa ttgccaggtg tctacaacct gagcgcgcgc
      961 aaaacaggta aataacagca gtagcgtgag aagcccttta ttcatcgcgc ttccttcccc
     1021 gccatccgct taacaatgcg cccgcaatca tcaacaatcc tcccccccag atccagcgca
     1081 caccgctttg tacatacaaa cgaaaagcgt aacgatccgc tccggttttc tcgcccatga
     1141 ccgcatacca gtcatggatg ccattccagc gaattgacgg ttccatcatt tgctgacgac
     1201 gtgcttcgta aaaacgccgc tccggcgtta attcaccaat gcgttgctga tggtcaaaca
     1261 gtgccactat cgctttttcg ctggtgtaat tgcctttggc ttgcagatcg aggcgctcaa
     1321 aacggaaggt gtatcctgcc agcgtcacct gctgacccgg ctgtaaattc aggctgatct
     1381 cctgacggct gacgctggaa accacaattc ccgcggcaaa taacagcacg cccgcatgcg
     1441 ccagcagcgt cggaagccgc gcgcgtttgc cagagacaaa cgtcgccagc acaatcacca
     1501 ccagcatcaa cagagcaaac ggtaacgtcg cgcggttaaa atacggcgcg ccaacggaga
     1561 ggcgtcccca gcccagcagg ccgtaaatca tcggataaag cgttcccacc agcacgatca
     1621 gtagcactgc gcaaaacagc aacagcgtag cgaggattaa catttcccgc gataaccccg
     1681 aaaaacgcac cgccgggcca ccgtcccgcg ctcgccagcc atacagagcc agagacgcaa
     1741 ggctaatcag tgcaaacagg ctgaacaacg gcacggcgcg gacgttatcc agcgcgaacg
     1801 catgaaccga aaccagaatg ccagaacgga caattaaggt gcccagcagc gacagcatca
     1861 gagttactat cgccagcaac agcgaccagt ggcggaaaat cccccgctgg cgtgtcaggg
     1921 ataaactgtg cagcagcgct gtggcagaaa gccagggtaa taacgacgcg ttttccaccg
     1981 gatcccagaa ccaccagccg ccccagccca gttcgcaata ggcccaccag gaaccgagga
     2041 tgatccccgc tgttaatgcg ctccagccag gtagtgccca acgccagcaa attcgggcgc
     2101 aggtaccacc aaactcgccg cgcagtaaac tcgccagcgc cacgctcgcc gccaccatca
     2161 aaccgccgta gccgagataa agcagcggtg gatgaaagat aagaccggga tgttgcagca
     2221 tcggattgag atcgcggcct tcgattgctg gtggaaatat ccgcacgaag ggatcggacc
     2281 acagcaccac aaacagtagc agtgtggcga gcatgagaga taaaacggct agcgtcagcg
     2341 gaaatagcgg atcggtttgc tgtcgataat gccaggcaaa cagcgcgctc caggcggaaa
     2401 gcagcagcac ccaaagcagc agcgaacctt catgaccgcc ccacaccgcc gccagtttga
     2461 gttcccacga cagcaggcta tagctatgtt gggcgacata aatgaccgag aaatcgctga
     2521 tgagaaaaca atacgtcagt acgccaaagg cgagcagcag gagcgcgaac tgcgccagaa
     2581 tgccgatgca agtgagtcgc atcatggcag gccagcgcaa ccgcactccc gcgaaggccg
     2641 tcaacggggt caacacgttg accccgagac ttaacaacag cgccagaaaa ctgacttcag
     2701 gaaggaatac gtccagaaac cacctctgtt ctggcttacg ccaccgttaa ctgcccggcg
     2761 tagagaatga aaaagcgcag catcaacacg ccgaccaggc tggccccaca tgccgccagc
     2821 acggcaggaa tgccggaact gcgattgacc cacggtttga gcaacattgg cacaatcagc
     2881 cccagcccgg cgacaccaag ccagaaccac cacgtccaga aaccgccgcc caatgccgcc
     2941 accagcgcac gcactttacc gtcatcgccc agcgccagac cgacaaaaaa cgccaccagc
     3001 aggaagattt caccccatac cacggggatt tccatacggt gtacaaagtg cgcttccgtg
     3061 gaatgcgggt tactgcgttg gcgtatcgcc atggcgatca gcgccaccgc cgcaccagac
     3121 gagatgccgg agaagaggaa cagcaccggc aggatcgggt tattgaggaa cggatacgat
     3181 ttcagcgccg acagcagaaa gccggtataa gcccccaaca acaccgccag caccagcatc
     3241 aatgtttcca gtccgcgatg aacgggcgtc agcaggctca agaccttttg cacgatcccc
     3301 agcttcggca accagcgttg ttgcagggca agcaaatcat gttcaaagat tttcgccagc
     3361 cacagcacca gcaccaccat gtaaagctga aacagcatca cccccatcga catcaccgag
     3421 gtaaaactgt agtggaacat cagcttccag aaggtccacg gtcttgtcag gtggaagacg
     3481 aggatcaaca gaccgaggat caccgcgcca ggcccgacaa tcagcgtggt gcgcaacaac
     3541 gtactgtctg caccgcccgc ctgcgggtag acacgacgta acagcacggc cagcgtcacc
     3601 agaccggcag aaataccaat caaaaacagg tagatggcaa tcggccagtc ccacaccagc
     3661 gattcaaaat gaaatgcgga agtctgcgtc attggctcac ctccccgtat ttaaacggta
     3721 cgcggtacag cttcggtttg gttcccagcg ccagcttgta gcggtaagtg ggcttctggc
     3781 gcagcagttg cgaaatctca ctgctgggat cgtccagatt gccaaacgtc agcgccttcg
     3841 tcgggcaagc ctcaacgcac gcgggcagct taccggcctg caaatgggtc ttacggcaga
     3901 aatcgcattt gtccgccgtc ttcgtgaccg gatggataaa gcgcacgcgg tacggacagg
     3961 cggcgatgca gtactgacaa ccgacgcaga gatccggatt aacatcgaca atcccactgg
     4021 cagcgtcgcg aaaagacgca ccggtcgggc aaacgtcaac gcacggcgca tgatcgcagt
     4081 gctggcaaga cttacggaag aagcgatatt tcacatcagg aaattcgccc tgcggctcgc
     4141 tacgaataat cgtcaggcgc gagacgcctt ccggcacctt gttcacttcc cgacaagcat
     4201 ccatacaggc ggtacagccg atgcataacg actcgtcatg caccatgccg taacgcaccc
     4261 cattgatatt caacgttttc gccaccacgc gtcctgcggt gccgctgacg gctgccagca
     4321 cgccgacgcc ggtgagaaac tggcgacgag accaggtcat ggctgctcct taagcaacgg
     4381 aacagacgcc gggttaaagt tcggattggt gcgctgatcg ctgtggcaat cgacgcaaat
     4441 cttaatccgt cctttgtcac ttaacgtctg catcgtatct tgctgtggat gcagggaatg
     4501 gcagctggcg cacgccactt tggttacgtg gacatcgtgc ggccagaagg ctttttgcaa
     4561 ctgttccggc agatggcagg acatacagac gctgttctgc tctccaacct tgtacatcgg
     4621 ctcgttaaag cgcatcacat ctttcacccc ttcgcggtgt tgcggtgatg gctgaccgtg
     4681 gcagttggtg caggtgaccg gcagtttgtt attcgggttg ataacagaag catgtttgcc
     4741 atgcatacct tcggtatctg gcttatgaca gtccagacag gcggcatccg ggttacgctg
     4801 ctgggtaact tcgtagcgat catcagatgc ctgcgccgca ggggtagcgg taattccgtt
     4861 caggctccac aacaggcccg acgccagcac cccggcagtt aataacgaac gtaatacgct
     4921 catattcact ccattgtgtt ttccgcccct tgcgaggcgg aacggggtta ttggcttaac
     4981 agaccgtttt tacgtgcctg ctcttcccac tgcgggatca ccgttttgat gaagtcctgc
     5041 ttctcggcct tgatttgctc catgttcagg ccaatggcct gctgggcttt ctctttggtc
     5101 gagatatccg ggatctggat ttcatgggtt atgcctttgg tcgccagcag gcgcgccagt
     5161 ttggtgcgtg catccgccgc tttatccatc gccgtaccga gcatcctcag accttcttcc
     5221 ggtgcgtgca tatgaatgcc gtgggaagcg atcgccaaat cccaacgcca ctgggcatga
     5281 cggatatcgt cctgaatcgg cttcatttcg gcttccgttg cgcctgcatc cagcgccgct
     5341 ttcgcttcga agtgagcgtg aaccagttga tcttcaacct tgattttcag gtcgttaatc
     5401 gactgcttac gttccgcgac cactttttgc aaggcagctt tgtcctgggt atggcagttc
     5461 gcacaagtct gggcgaagtt atcaaacgga ttaccaattt tatggtcggt gtagagtttg
     5521 ccttcggcgt tctgcacttt tggcatatgg caatcgatac aggtcacgtt gtttttgccg
     5581 tgaatgcccg ctgtccaggt ttcatattcc gggtgctgcg ctttcagcat tggcgttttc
     5641 gacagggagt tagtccagtc agagaaggca attttgtcgt aatactgctc catattttcg
     5701 actttcatgc cgtcatccca cgggaattta accgctttgt ttttgccgtc gaagtaatac
     5761 tccacatggc actgaccgca aaccatcgat tgctggtcga aacgtccggc tttctcgaaa
     5821 ggtttaccaa tggcttccat cgcgcgagcc gcatacggac gggaaagggt taactccggt
     5881 ttgcctttgg cgaactctgg tgaggcggtg ttatggcaat cggcacaacc taagttgttg
     5941 acgatttccg ggccgccgcg cgcccatttt ccgtggaagt agccatcttc gccgtctttc
     6001 tggatcagac gcgccacatc cgggctttta caactccagc acgccatcgg tagcggacca
     6061 tcttcagcgt ttttcggcgc accggtacgc aaggtttcac gcacatcggt cacagcaaag
     6121 gcatgtccac gcggcttgtt gtaatcgcgc gagaagggat accctgccca caggatcacc
     6181 agccgtggat cttccgccag ggcgtcaaca cgctctgact gttccgaggt agctttccag
     6241 gagagatatt gatcgggatg ctgcggggca aaggtttcat tcttcgcttc cacagttaca
     6301 ggtgttgcgg gagcagccgt ttgttcagcg tgaacagaag tgaaaaagaa aaaaggaatc
     6361 aataagctga agatacggcg tgcgtttatt tttatccttg tcatagggct tcatccgaat
     6421 tgcgccattg ttgcaatggc ggtttttatt gtttttcacg acagtaaccg cacctacact
     6481 gtcatgacat tgctcgtccc tatgtgtaac aaataaccac actatgaatg ttgtctttaa
     6541 tcaattgtaa gtgcatgtaa aataccactt tagagttagt cagtatcttc ctctttttca
     6601 acagcatgca taactgcatg ttcctcaaag aattaatcaa cttttgttgc tgaccttcaa
     6661 aaattaccct gccgtttatt tgcacaattc tacttttgcg tgatctgtcg cccaaatact
     6721 aaacaaaact gccaataccc ctacatttaa cgcttatgcc acatattatt aacatcttac
     6781 aaggagaaca aaagcatgag tcaaattcac aaacacacca ttcctgccaa catcgcagac
     6841 cgttgcctga taaaccctca gcagtacgag gcgatgtatc aacaatctat taacgcacca
     6901 gataccttct ggggcgaaca gggaaaaatt ctcgactgga tcacaccgta ccagaaggtg
     6961 aaaaacacct cctttgcccc cggtaatgtg tccattaaat ggtacgagga cggcacgctg
     7021 aatctggcgg caaactgcct tgaccgccat ctgcaagaaa acggcgatcg taccgccatc
     7081 atctgggaag gcgacgacgc cagccagagc aaacatatta gctataaaga gctgcaccgc
     7141 gacgtctgcc gcttcgccaa taccctgctc gaaatgggca ttaaaaaagg tgacgtagtt
     7201 gcgatttata tgccgatggt accggaagcc gcggtcgcga tgctggcctg tgcccgcatt
     7261 ggcgcggtac attcggtgat tttcggcggc ttctcgccgg aagccgttgc cggacgcatt
     7321 atcgactcca gttcacgact ggtgatcact tccgacgaag gtgtgcgtgc cgggcgtagt
     7381 attccgctga agaaaaacgt tgatgacgcg ctgaaaaacc cgaacgtcac cagcgtagag
     7441 catgtggtgg tactgaagcg tactggtgga aaaattgact ggcaggaagg gcgcgacctg
     7501 tggtggcacg acctggttga gcaagccagc gctcagcacc aggcggaaga gatgaacgcc
     7561 gaagatccgc tgtttattct ctatacctcc ggttctaccg gtaagccaaa aggcgtgctg
     7621 cacactaccg gcggttatct ggtgtacgcg gcgctgacct ttaaatatgt ctttgattat
     7681 catccgggcg atatctactg gtgcaccgcc gatgtgggct gggttaccgg acacagttat
     7741 ttgctgtatg gcccgctggc ctgcggcgcg accacgctga tgtttgaagg cgtaccgaac
     7801 tggccgacgc ctgcccgtat ggcgcaggtg gtggacaagc atcaggtcaa tattctctat
     7861 accgcgccca cggcgattcg cgcgctgatg gcggaaggcg ataaagcgat cgaaggcacc
     7921 gaccgttcgt cgctgcgcat tctcggttcc gtgggcgagc caattaaccc ggaagcgtgg
     7981 gagtggtact ggaagaagat cggcaacgag aaatgcccgg tggtcgacac ctggtggcag
     8041 accgaaactg gcggtttcat gatcacgcca ctgcctggcg ctaccgagct gaaagccggt
     8101 tcggcaacac gtccgttctt cggcgtgcaa ccagcgctgg tcgataacga aggtaacccg
     8161 ctggaggggg ccaccgaagg cagtctggtg atcaccgact cctggccggg tcaggcgcgt
     8221 acgctgtttg gcgatcacga acgttttgag cagacctact tctccacctt caaaaatatg
     8281 tatttcagcg gcgacggcgc gcgtcgcgat gaagatggct attactggat caccgggcgc
     8341 gtggacgacg tgctgaacgt ctccggtcac cgcctgggga cggcagagat tgagtcggcg
     8401 ctggtggcgc atccgaaaat tgccgaagcc gctgtggtcg gtattccgca caatattaaa
     8461 ggtcaggcga tctacgccta cgtcacgctt aatcacgggg aggaaccgtc accagaactg
     8521 tacgcagaag tccgcaactg ggtgcgtaaa gagattggcc cgctggcgac gccagacgtg
     8581 ctgcactgga ccgactccct gcctaaaacc cgctccggca aaattatgcg ccgtattctg
     8641 cgcaaaattg cggcgggcga taccagcaac ctgggcgata cctcgacgct tgccgatcct
     8701 ggcgtagtcg agaagctgct tgaagagaag caggccatcg cgatgccatc gtaacccaca
     8761 attgccggat gcgctccggg caacaagtcg gaaacgattt gtaggcctga taagacgcga
     8821 cagcggcgca tcaggcgctg gttgccggat gcggcgtgaa cgccttatcc ggcctacatt
     8881 cggcaacggc tgtaggcctg ataagacgcg acagcgtcgc atcaggcgtt gattgccgga
     8941 tgcggcgtga atgccttatc cggcctacat tcggcaaggg ttacccaagc gttaaccttc
     9001 tcccataagg gagcgggaat taaaacaatc cctacattac ctctggagaa tctgtgatga
     9061 atggtacgat ttatcagcgg atagaagaca atgcgcattt cagggagtta gtcgaaaaac
     9121 ggcaacggtt tgccaccatc ctgtcgatta ttatgctggc agtttatatc ggctttattt
     9181 tactgatcgc cttcgcgccc ggctggctgg gcaccccgct gaatccgaac accagcgtca
     9241 cacgcggtat tccagtcggt gtcggggtaa ttgtgatctc ctttgttctc actggtatct
     9301 acatctggcg ggcgaacggc gaattcgacc gtcttaataa cgaagtcctg catgaggtac
     9361 aagcatcatg aaaagagttc tgacggcgct tgccgccaca ctccctttcg cagctaacgc
     9421 cgcggatgct attagcgggg ccgtagagcg tcagccaacg aactggcagg cgattattat
     9481 gttcctgatt ttcgtcgtgt ttacgctcgg cattacctac tgggcgtcaa aacgcgtacg
     9541 ttcgcgtaat gactactaca ccgcaggcgg caatatcacc ggttttcaga acgggctggc
     9601 gattgccggg gactatatgt ccgccgcctc attcttgggg atctccgcgc tggtgtttac
     9661 ctccggctat gacggcttaa tttactcgct aggcttcctg gtaggctggc cgatcatttt
     9721 gttcctgatt gccgaacgtc tgcgtaacct cggtcgctac acctttgccg atgtggcctc
     9781 ttaccgtctg aaacaagggc cgattcgtat tctttcggcc tgtggttctc tggtggtggt
     9841 ggcgctttac cttatcgccc agatggtggg cgcaggtaaa ctgatcgagc tgctgtttgg
     9901 ccttaactat cacattgcgg tagtgctggt cggcgtgctg atgatgatgt acgtcctgtt
     9961 cggcggcatg ctggcgacca cctgggtaca gattatcaaa gccgtgctgt tgctgttcgg
    10021 tgccagcttt atggccttta tggtgatgaa acacgtcggc tttagcttca acaatctgtt
    10081 cagcgaagcg atggcggtac acccgaaagg tgtcgacatc atgaagccgg gcgggctggt
    10141 gaaagatcct atctccgcgc tctctttggg gctaggactg atgtttggta cggcgggctt
    10201 gccgcatatt ctgatgcgct tctttacggt cagcgatgcc cgcgaagcac gtaagagcgt
    10261 gttctacgcc accggattta tgggctactt ctacatcctg acctttatta tcggctttgg
    10321 cgcgatcatg ctggtcggtg cgaatccgga atataaagac gcggcgggcc atctgattgg
    10381 tggtaacaac atggcagcag ttcacctggc caatgcggtg ggcggcaacc tgttcctcgg
    10441 ctttatttca gcggttgctt tcgccaccat tcttgcggtg gttgcgggtc tgacgctggc
    10501 gggcgcatcg gcggtgtctc acgacctgta cgctaacgtc ttcaaaaaag gtgcgaccga
    10561 acgtgaagag ctgcgggtat cgaaaatcac cgtactgatc ctcggtgtga ttgccattat
    10621 cctcggcgtg ctgtttgaga atcagaacat cgcctttatg gtggggctgg cgtttgccat
    10681 cgcggcgagc tgtaacttcc cgatcattct gctttccatg tactggtcga aactgactac
    10741 ccgtggcgca atgctgggcg gctggttagg actgattact gcggtggtgc tgatgatcct
    10801 cggcccgact atttgggtac agatcctcgg tcacgaaaaa gccatcttcc cgtatgaata
    10861 cccggcgctg ttctctatca gcgtggcatt cctcggtatc tggttcttct cagcaacgga
    10921 taattcagcg gaaggtgcgc gcgagcgtga actgttccgt gcgcagttta tccgctccca
    10981 gactggcttt ggcgttgagc aaggccgcgc gcattaatct ctccccttcc ccggtcgctt
    11041 gatcggggaa tttcttcaga acatcatcct ggccaccagc ggcgaaacca ataccatcaa
    11101 tacacctgaa agcatcatga ccaggcttgc aactacgcct tcttgttgac ccagttcata
    11161 cgaccgcgca gtcccggcac catgagatgc cgcgccgaat ccagcacctt tcgccatccc
    11221 ttcgcgaata gagagacgta aaaacaacac gtcgccaatc gccatgccaa acacaccggt
    11281 gaccaccaca aacagcgcca ccagatcagg ctgtccgccg agcgactggc tcgctgccaa
    11341 agcaaatggc gtagtgaccg aacgcaccgc caggctacgc tggatctcat ctggcagggt
    11401 aaacaatcgc gccagccaca ctgagctggt cactgccacc accgttgccg tcagtacgcc
    11461 tgccgtcaac gacatccagt gacgcttaat gatcgcccga ttgtcataca ctggtaccgc
    11521 aaaggcgatg gtggccggac ctaatagcca tagtaaccag tgcgcttcgc caatatagtt
    11581 ttgccaggaa atattgccaa aaaccagcag catgaccagt agcgtaggcg tcagcaccag
    11641 aggcatcaat ggcaatttac gaaagcggcg ataaaggcgt ttgttaagaa aatagataac
    11701 tagcgtaatt gccagacaga gcatacttag ctgaaaatta gccacggttt aacctgcgca
    11761 tttcatagcg atagactttt tccactaccc aggcagtcgt gcccagcacc atcaccgtac
    11821 tcagcgcgat cacagcaaaa atacgccagc catccaccag taatagttgt gcatagttca
    11881 ccacagccac cacggcaggg acaaaaaaca gcaacatttc ggccaatagc caacgcgccc
    11941 cggctcgaac ccattgcaga ggaagaatgc gacaaacaat cagcgccagc atcaggatca
    12001 tccccaccag gttggcaggc agcggcaaat gcagccagga gacgagatac tgcgcaaaaa
    12061 taaataaacc tgcatacagc acaacctgaa aaaatacctg gatagattgc actacggcag
    12121 gggtaatgcg gcgaagcgcc acgggcatga gaggatatcc tgaaaataaa acagaatggt
    12181 aagtatagag agcgctcgcc cctgactgaa atgaattaaa atcattgcag gtatagtcaa
    12241 agggaatagt tatggatatc agaacgctgc gttattttgt cgaagtcgtg cgccagcaaa
    12301 gcttcacccg cgcagcggag aagttgtttg tcacccaacc aactatcagc aagatgctga
    12361 agaatctcga agatgagctg aactgtatat tgttgatccg cgatggtcgt cggctgcttc
    12421 tcactgatac tggtcgcgtc gtatttgagc gcgggctggc tttactggct gagtttcggc
    12481 aactggaagc tgagcttagc gatatcaatc agcttaaaac cggcgttttg cggctgggaa
    12541 tcccgccgat ggttgggatg ttgatggccg ggccgattgg cctttttcgc cagcgttacc
    12601 ccggcgtaga attgaaaatt tcggagtttg gtggtctgac ggtacaacaa gcggtgatga
    12661 acggtgaact ggatatggca ctgacagccc tgccagtaga agaaagcggc ctgacaacgt
    12721 tatcgctgtt tagtcatcca ctatgtgtac tgacgccaca ttcaggtaaa tggcttgaat
    12781 gtgatgccgt ctcaccggag acattggcgg aacatcctct acttatctat aacgaagatt
    12841 ttgccttaag taagcaattg atgaagttat tcgatgaaca tggcgttaag ccgagaattg
    12901 cagttcgcag cgggcaatgg gattttctcg ccgcgatggt tcaggccggt gtcggaatag
    12961 ccatattgcc ggaacccatt tgccagcgat tagataaaaa caccttaaaa tggctaccgc
    13021 tggaaagcga actaagctgg aaattaggaa tgatttggcg tgagggcgtt tacctttccc
    13081 actctgccca cgcgtggctg agctgttgtg aaggatactg gttaacgcca gagtaagtcg
    13141 ggttggcggg ctcgcccgcc aaccatgcag attactgatt ttcctcaatc agcaacgctt
    13201 caagcaaatc gagatcgtgc agtaattttt gcagcgtttc gttactgatc tcccgcgtgg
    13261 cgcgcaggtg gtacagttca gcacgttcag aacgcaatgc cgccagacgg aagcgacgct
    13321 ccaggttctc ttccaggatt gaactttcta catcattacg tccatcggcg cgacgacgca
    13381 ggttaccaat gacgcgagaa ctaacctctg tgagcagctg gttatcgata ttctcttcgg
    13441 tatccgccgc cagacgctcc tccattttct ggatcgccac aatcgccact tctgccgttg
    13501 ccgctcgtgc aatacgttcc tctttcaatt gttgcgaatg gtccgcgact tctatgtgtt
    13561 gtagcagaat aggcaacatc accacgccga caaacaatga gaagagaatg acgccagccg
    13621 ccaggaacac caactcatag cgcgcaggga agacgttacc atccggcaag agaagcggga
    13681 tagagagcac accggccaga gtgatcgccc cacgcacccc ggcgaaagac gcgatcagga
    13741 tttctcgtgt agtccacgaa ccaaactcca tcggcttctt cttcaggaag cggttgctga
    13801 actttttcat cgtccacagc cagccgaaac ggaccagcat cagcgccgca tatatcagaa
    13861 taatatcggt aaacagcatc cagatttcga cgttagggtc gatttctgcc gccatcagcg
    13921 acgtctccag aatccccggc aactgcagac ctaacagcag gaacaccatg ccgttaaaga
    13981 caaattccag catcgcccag gtgctgtttg cgcgcaggcg cattgccagc ggcgcacggc
    14041 gcatcacacc ggagcgggtg atggtcatcc cggcggcaac cgcagcgagg atgccggaaa
    14101 cgccaatatg ttccgcaatc agataagaag cgaatggcag caagaacagc agaacgatct
    14161 gcgtcgcggg ttcatcaccg ccccagcggc tgaggaatcg cagcgagcga ccatacagcc
    14221 agctcaccac aaaaccggcg agaataccgc ctattgccac tttcataaat tcgaccgtcg
    14281 caccgccgac ggtgaagatc attgtcccca tcgccactgc cacggcaaac ttcaacgaca
    14341 ccaggccgga ggcgtcattc atcaacgctt cgccctgcaa aatgcccatg atttttttcg
    14401 ggatgcgccc ctcgccaaca atcccggaga gcgctacagc atcggtcgga gaaagcaccg
    14461 cagccagcgc aaaggcgggg atcagcggaa tgcccggcac cacccagtaa ataaggaagc
    14521 caatgccgac cacggtgacc accaccagcg ccagcgcgag gccgaaaatc tctcgcccat
    14581 gttcgagaaa ttcacgggtc ggcgttttcc agccatcagc gaacagcaac ggcgggataa
    14641 acaagactaa aaagagttca ggatcaaact ccacatgcaa accaaacgtc ggccacgcca
    14701 gtagcgcacc gatggcgatt tgcataagcg gaagcgggat ctgaaagggc atgacacgag
    14761 tgaccacccc ggacagcgag accacgaggg tcattatcag tatggtgaag aagatttcca
    14821 tgcgttccct gtttgcgatg ttttttatga tctgagaaag gcgttatgcc tgaattctca
    14881 ttttttcaga atatcgcaaa agctcacaca atgtgcggag aaaataaaaa cgggcagcca
    14941 ttgccacccg ttttcagaat cctatgtaag attagatagc ccagccaccc gcatagaagg
    15001 tcaccagcgc cacggcaata acgaccgtac cgatgttcaa cttgcgccat tcgccggaaa
    15061 ccagacgacc aatcaccaga gtcgcgaagc caatcatgat gcctgttacg atgttacagg
    15121 tcagcacgat gaataccgcc gtaaccagac ccgccatcgc atcaacaaaa tcagcaaagt
    15181 cgattttcgc cacgttgctc agcatcagca ggccgacgta catcagcgcc ggagccgttg
    15241 cgtaccccgg aacgaggtaa gagagcggag agaggaacag aatcagcagg aacagcacgc
    15301 caacagtgat agccgtcaaa ccggttttac cgcccgccgc cgtacccgcc gcagactcga
    15361 tgtataccgc tgccggagct gcacccacga ggccagaaaa aacgctgctc atggagtcgg
    15421 tggtcagtgc tttcccgccg tcgatgatct gcccatcttt atccagcagg ttcgcctgac
    15481 cggcgacggc acggatagtt ccggtggcat caaatactgc cgtcatcacc agtgccagaa
    15541 cacttggcag gactacagga ttcagcgcac ccataatgtc caggctgcca atcagtgaat
    15601 tgccgttttc atcgctcaat gaaggcatgg cgaaaacgcc ggagaaatgg acgttaggat
    15661 cgaagatcaa accgacaatt gagataccga taatggtcag cagaatgcca ccagggactt
    15721 tcagtttttc caggccgata atcactgcca gccccaccaa cgacatcatc accggaaagg
    15781 tcgtgaaatc ccccagcgcg acgggcagac catcaagtgg gtttttaatc accagaccga
    15841 caccattagc agcaatcagc agcaggaaca gaccgatacc aatccccgtg ccgtgcgcca
    15901 caccgtgcgg caagttgcgc aaaatccagc tacggatacc cgtggcagaa attaccgtaa
    15961 acagcacacc catcaggaac acggcaccca gcgcgacagg tacgctaata tgttgcccca
    16021 gcaccagact gaatgcggta aacgccgtca gagagatggc gcaaccaatc gccaacggca
    16081 gattagccca cagacccatc acgatagaac cgagtccggc aaccagacag gttgcaacga
    16141 aaactgccgc aggcgggaag cccgctttac ccaacatgcc tggaacgacg ataaccgagt
    16201 agaccatcgc cagaaacgtt gttaacccgg caaccacttc ctgacggaca gtgcttccac
    16261 gttgtgaaat tttaaaccag gcgtcgagtg aaccgccggt acgcgctgat ggcgtagaca
    16321 taagaaaaca tcccctgaga gtttaatttt cgtcaagatg cgtggcggac aatcccctgc
    16381 cagcgaaacg ttatcttcct gctccaggtt tgcgacaatt tgctgcgtcg caaaaaaaag
    16441 caaacgttta gctccgcgca tacaaaacgg gtggtaaaaa aagcaaacga ttatccggcg
    16501 atcatagcgg ctttttcaac cgaagtttgc tgcttttttc tcttttattg tcttgacgat
    16561 gaaaaaccag gcaattgatg cgcaaacgtt atcgtcgatg cgcaagattg cagcgttatc
    16621 gagccgtata ccgttcagga ttgcgataaa aaatcgtctg agatgacaaa aatattgcgg
    16681 ctaaaaagtg tacttttaca ctggcgaaga aacgccggtc acatacaatt aaagcatcaa
    16741 caccaaccgg aacctccacc acgtgctcga atgaggtgtg ttgacgtcgg gggaaaccct
    16801 cctgtgtacc agcgggatag agagaaagac aaagaccgga aaacaaacta aagcgccctt
    16861 gtggcgcttt agttttgttc atcttccagt aagcgtgcgc cggtaccttc ttctcctaat
    16921 cggtcgcccg ggttacgcaa cgggcaatca ctgcgcgaaa ggcagccaca accaatacat
    16981 ccatccagtt cgtcacgcag cgccactaag gtatgaatgc gccgatccaa ctcttctcgc
    17041 cattgggacg aaagctgttt ccactctttc gcacttaacg tatgcccttc tggcaacacg
    17101 ccaaacgctt cacgaatggt tgcgagcgga atgccaatac gctgagcaat tttgataatt
    17161 gcgacatatc gcaacacatc acgtttatat cgccgctgat tgccgctatt acggatactg
    17221 gtaatcaacc ctttgctttc atagaaatgc agcgccgata ccgccacacc gctgcgtttc
    17281 gccacttcgc cgggggttag cagcgcttta atgcggggta atttcttttc cataaatcgc
    17341 tttacctcaa gttaacttga ggaattatac tccccaacag atgaattaac gaactgaaca
    17401 ctgaaaagag gcagatttat gtcccatcag aaaattattc aggatcttat cgcatggatt
    17461 gacgagcata ttgaccagcc gcttaacatt gatgtcgtcg caaaaaaatc tggctattca
    17521 aagtggtact tgcaacggat gttccgcacg gtgacgcatc agacgcttgg cgattacatt
    17581 cgccagcgcc gtctgttact ggccgccgtt gagttgcgca ccaccgagcg tccgattttt
    17641 gatatcgcaa tggacctggg ttatgtctct cagcagacct tctcccgcgt tttccgtcgg
    17701 cagtttgatc gcactcccag cgattatcgc caccgcctgt agttttattg cccgcgcgtt
    17761 aactcccgcg cgggtaatta ccccatccat tgcataaaca cctgcggcgg catcgccttc
    17821 gcaaaaaacc atccctggca atagcgcacg ccgcgtttgc gcagccagtt aacctgcgcc
    17881 tcagtttcga cgccttcagc gatcgttttt aaccccaggc tgtgcgccag ctcgatgatg
    17941 tgttccgcaa tcaaatgact ggttttgtgg gtggtcagcg tctcaacaaa cgatttatcg
    18001 attttcaaaa tatcgacatt caatgattta aggttatgca agttagagta gccaatacca
    18061 aaatcatcaa ttgccacttc gtaaccggcc ttgcggaaag ccagaataat cggcgtcatt
    18121 ttgtcgacat caagaaatgc atgttcagtc acttcaaatt taatttgctg cgggcgcacc
    18181 gcatgttgct ctgttttctg attggttcgc gctatcaacc gtgaggtatg aaaatcggag
    18241 gctgacaggt taatagaaac atagcgatcg gtatgtgttg ccagatacgc gcccaaatca
    18301 cggaagacat tatcaataac ataatcagtt acctgttcga tcatcccctc cttttctgcc
    18361 agcggaataa actctgccgg attcattact tgcccctgct caccaggcca acgtaacaac
    18421 gcttcagcgc cgatacattt ttctgttttg atatcgatta ttggctggta ataaaggcaa
    18481 agttgatttt tttcgagggc gcgttgcaat ttgcgtttgg gagacaaata gttttgtcga
    18541 atacgtagcc agagtaatag cagaacaaga ctccccaaaa taccggcggg caacgcaaaa
    18601 ataagataat tataaaaatg agttataaga cgttgatatg aagtcgcaac aatggctgca
    18661 attgggcgtt ttgtcgaata aactgtcgca tataaatagc catttcgttg tacggttaaa
    18721 tcattcaaat gaatcagcgg agaaaacgtt gctgccgagg cctcgttgct taacgagaaa
    18781 aaggttttcg tcaccgtatc atacacaccc cattgcaatg tcggatcatc agacatcact
    18841 tcactccaga agagagggtt gataaccgcc acataatttc cccgctgcat gtaggtcatt
    18901 ttatagccag agaaaaaagg cgtatcacgg taataataga tagaaacgtt aggttcacgc
    18961 ttataatcgg ccggtgcaat cgtatagcca tttacagacg ctatcagcga tgagcataaa
    19021 aaatggttat cacgagcata aatcaattca ttaatataaa gatagccacg aataatattc
    19081 aacattcgct tttgatgggc tggagtgcaa acttgcccct gatagcgttc agctgcatcg
    19141 ctcactaaat ctgcctgacg aatgaccagc tcagatttat ccaacgccag ctgagcaaag
    19201 gttcggagct gattattcac ttctgatttt gcccaaaaga aggcaatcca tagcgacaga
    19261 atgatgggaa agagaactaa aaagataatg cccggaaacg ccagtaattg gtgtcgtgca
    19321 cgatgactca ttgctatcaa ttccctgcct gtttataccc ggttattgca aggtgaaacc
    19381 ctggctaccg acgtctttag ataaatctta cggtataaat agcacttttg cctgatataa
    19441 aattgagacc tttttaatcg tcaccaggat gacgataatt cattgatctt aaaaattaat
    19501 caccttcagg aatgcagagg cggcgggaag atataccctt aatgccgctg aaaaaacaca
    19561 taatctgtac ggcaataaaa gtcacggcct aatctggcta acaaacaacc aattgtgata
    19621 tagttcacaa aattcatgaa acaaacagag tgtttcattt ttgtgttctg caagccccgc
    19681 gatagccaga gtgtatcacg cctcccgtga acaacgccgc gctgtccagg gcatcagctc
    19741 ttttccgagg aattgtttga tggctaccct caccactggc gtggttcttc ttcgctggca
    19801 acttcttagt gccgtaatga tgtttctggc tagcacgctc aacatccgtt ttcgtcgggc
    19861 tgattatgtc gggcttgcag tgatcagcag cggtctgggc gtagtttccg cctgctggtt
    19921 cgcaatgggg ttgcttggca tcacaatggc ggatatcacc gccatctggc acaacatcga
    19981 gtcggtgatg atagaagaga tgaatcagac accgccacaa tggccaatga ttttgacttg
    20041 atatgtagaa gcctccataa cggaggcttc ttttttatgg ctggcagatg ctttaatcat
    20101 ccaccttaaa acaatatacc ctattgtttt aaagacaaat cagaacggga tatcgtcatc
    20161 aaagtccatc ggcggctcgt tagacggcgc tgccggagcg gactgctgcg ggcgagactg
    20221 cgcgccgccg ctgaactgat tgccaccctg cggctgctga ggctgacccc aaccgccctg
    20281 cggctgacca ccaccgatat tgccacctgc aggagcgcca ccaccctgac gaccacccag
    20341 catctgcatg gtgccgccaa cgttcaccac gacttccgtg gtgtagcgat cctgaccgga
    20401 ttgatcggtc catttgcggg tacgcagctg accttcgata taaacctgag aacctttacg
    20461 cagatattcg ctggctactt ctgccagttt gccgaacagc acaacgcggt gccattcagt
    20521 ctgctctttc atctcgccgg tggctttatc acgccaggat tcggaagtag ccagcgtaat
    20581 gttggcaact gcgccaccat ttggcatgta gcgtacttcc gggtcctgac ccagattacc
    20641 aacgagaata accttgttta cgcctctgct ggccatgttc gtgtctcctg aaaaaaaatc
    20701 gttctgaata agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt
    20761 gacctcggtt ccgggaaaca aacctggcca gacattgtta cacaacactc cgggtaatgc
    20821 attccaatac tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc
    20881 cggtagcacc atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga
    20941 tcgaagttcg gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg
    21001 acaagcttat tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca
    21061 ccttatatgc cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt
    21121 ttctgtcact gatggagaag ccggacgtcg accatattga agggctttct cctgccatct
    21181 caattgagca gaaatcgacg tctcataacc cgcgctctac ggtggggaca atcaccgaaa
    21241 tccacgacta tttgcgtctg ctgtacgctc gcgttggtga gccacgctgc ccggaccacg
    21301 atgtaccgct ggcggcgcaa accgtcagtc agatggtgga taacgtgttg tcccagccgg
    21361 aaggcaagcg tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca
    21421 aaacgctgga gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct
    21481 gcgatctttc cgatccgccg aaactggaat tgcaaaagaa acataccatt gaagtggtgg
    21541 ttgatcgctt caaagtgcgt gacgatctta cccaacgtct tgccgagtcg tttgaaaccg
    21601 cgctggagct ttccggtggt accgcggtag tagcggatat ggacgacccg aaagcggaag
    21661 agctgctgtt ctccgccaac tttgcctgcc cgatttgcgg ttacagtatg cgcgagctgg
    21721 aacctcgcct gttttcattt aacaacccgg cgggagcctg cccgacctgt gacggtcttg
    21781 gcgtacagca atatttcgat cctgaccgcg tgatccagaa tccggaattg tcgctggctg
    21841 gcggtgcgat ccgtggctgg gatcgccgca acttctatta tttccagatg ctgaaatcgc
    21901 tggcagatca ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc
    21961 ataaagtggt gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc
    22021 gtggcgatac ctccattcgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc
    22081 gttataaaga gacggaatcc agcgcggtac gtgaagaatt agccaagttt atcagcaatc
    22141 gtccgtgcgc cagctgcgaa ggaacccgtc tgcgtcggga agcacgccac gtgtatgtcg
    22201 agaatacgcc gctgcccgcc atctccgaca tgagcatcgg tcatgcgatg gaattcttca
    22261 acaatctcaa actcgcaggt cagcgggcga agattgcaga aaaaatcctt aaagagatcg
    22321 gcgatcgtct gaaattcctc gttaacgtcg gcctgaatta cctgacactt tcccgctcag
    22381 cagagacact ttccggcggt gaagcccagc gtattcgtct ggcgagccag attggtgcag
    22441 gcctggttgg cgtgatgtac gtgctggatg agccgtctat cggcctgcac cagcgcgata
    22501 acgagcgcct gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg
    22561 tggagcacga cgaagacgcg attcgcgccg ctgatcatgt gatcgatatc ggtccgggtg
    22621 cgggtgttca cggcggtgaa gtggtcgcgg aaggtccact ggaagcgatt atggcggtgc
    22681 ctgagtcgtt gaccgggcag tacatgagcg gtaaacgcaa gattgaggtg ccgaagaaac
    22741 gcgtacccgc caatccagaa aaagtgctga aactgacggg cgcacgcggc aacaacctga
    22801 aagacgtgac gctgacgctg ccggtaggtc tgtttacctg catcacaggg gtttcaggtt
    22861 ccggtaaatc gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg
    22921 gtgcgaccat cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata
    22981 aagtgatcga tatcgaccaa agcccaattg gtcgtactcc gcgttctaac ccggcgacct
    23041 ataccggcgt gtttacacct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc
    23101 gcggctatac gccaggacgt ttcagcttta acgtccgtgg cggacgctgc gaggcctgtc
    23161 agggcgacgg cgtgatcaaa gtggagatgc acttcctgcc ggacatttac gtaccgtgcg
    23221 accagtgcaa aggcaaacgc tataaccgtg aaacgctgga gattaagtac aaaggcaaaa
    23281 ccatccacga agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc
    23341 cagctctggc gcgtaagctg caaacgttga tggacgttgg cctgacgtac attcgcctcg
    23401 ggcagtccgc aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc
    23461 tgtcaaaacg cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc
    23521 acttcgccga tattcagcaa ctgctcgacg tactgcataa actgcgcgat cagggcaata
    23581 ccattgtggt aattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc
    23641 tgggaccgga aggcggcagt gggggcggcg aaatcctcgt ctccggtacg ccagaaaccg
    23701 tcgcagagtg cgaagcttcg catacggcgc gcttcctcaa gccgatgctg taatcgttaa
    23761 ggccgctttc agagcggcct tcttctttca gagttgcacc agcaatttac gtttttcttc
    23821 cggcagcaaa ttcaccgcct gctgataaga cgcatccacc agataataga tttgcgaatc
    23881 cggcagcgaa ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggcttgg
    23941 acgcacatcg ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga
    24001 aacagctggg cgattttcta cttctttcac catcgcaaac agcacatctt ccactttgat
    24061 ctgcgtcgct ttccagtcgt tatgcacgct ctgttctgcg cctggttttg ccatgcaata
    24121 ttgtagcaac tccgaaatgg tcatttttta ctccccttga agtgtcgcga tgatgcgacg
    24181 cgatccgccg tggatgcgat gttcccccag ccagatgcct tgccaggcgc cggtctgaat
    24241 acgcccttta tgcaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga
    24301 aggcatatcg tctgctccct catagtcatg ctcataattt ccgttatcgg gaacggcgcg
    24361 gaggaaaaaa cgctccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag
    24421 agaggcggag gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc
    24481 agccagctga ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag
    24541 cgtgagcgtc ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg
    24601 cagcaagaca gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagtattac
    24661 gctctcccgc tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct
    24721 tgtggcaagg gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg
    24781 acatcgcgtg cggcggtaat atcgttatcg gaatcaccat aaaaaattcg gatattttta
    24841 tcctgcagcc attgcgattt tgtattttgc cctggtttat cgcccgcaaa gatcaccgga
    24901 ttcatgttgg tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt
    24961 ttggtcgggc tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca
    25021 atcagctggc gagcgacctc ttttggaatg ctgaattcat cccagccatt gttcattttt
    25081 tcccagaaca caggattctt cagataatct tcgctttctg gcgagaaggt ttttttgccg
    25141 cgccagaaac ccggactgga aaaaagtacc gtatcatcga tatcaaaccc caccgccatt
    25201 ggcggacgtc ctgcgaggct attttcaatt tgtgcaaccg aaacccaatg aattggcgcc
    25261 tgttcagcga gtttggctac gttagtaccc ggattaagcg gtgaaggaga tgaggccagg
    25321 gcaacagcgg aactgtttag agcgaacaat aagcaaacgg cactcaatgc ctgtgtgatc
    25381 ttgcgcatat ttttccctaa atagtcagta agttgaaact tattattgtg cgtttgttga
    25441 aaaaaccatc tgaccataac gccaaccgcg ggggaaagga aggattatct gcggatttgt
    25501 cacataaaaa gagcatgatc acatttggaa tacctgtgcg ataaaccaca cattgccacg
    25561 gttattctgc ttacacattc acacaatggc a
//
LOCUS       DADSZD010000059        25361 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.059, whole genome shotgun sequence.
ACCESSION   DADSZD010000059 DADSZD010000000
VERSION     DADSZD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.059"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(28..1887)
                     /gene="gss"
                     /locus_tag="J1Z28_002121"
     CDS             complement(28..1887)
                     /gene="gss"
                     /locus_tag="J1Z28_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBA7716956.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2092..2958
                     /gene="yghU"
                     /locus_tag="J1Z28_002122"
     CDS             2092..2958
                     /gene="yghU"
                     /locus_tag="J1Z28_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBA7716957.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMSWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3091..3339)
                     /gene="hybG"
                     /locus_tag="J1Z28_002123"
     CDS             complement(3091..3339)
                     /gene="hybG"
                     /locus_tag="J1Z28_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBA7716958.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3352..3693)
                     /gene="hypA"
                     /locus_tag="J1Z28_002124"
     CDS             complement(3352..3693)
                     /gene="hypA"
                     /locus_tag="J1Z28_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBA7716959.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3686..4174)
                     /gene="hybE"
                     /locus_tag="J1Z28_002125"
     CDS             complement(3686..4174)
                     /gene="hybE"
                     /locus_tag="J1Z28_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBA7716960.1"
                     /translation="MTEEIAGFQTSPKVQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4167..4661)
                     /locus_tag="J1Z28_002126"
     CDS             complement(4167..4661)
                     /locus_tag="J1Z28_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBA7716961.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4661..6364)
                     /gene="hybC"
                     /locus_tag="J1Z28_002127"
     CDS             complement(4661..6364)
                     /gene="hybC"
                     /locus_tag="J1Z28_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBA7716962.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6361..7539)
                     /gene="hybB"
                     /locus_tag="J1Z28_002128"
     CDS             complement(6361..7539)
                     /gene="hybB"
                     /locus_tag="J1Z28_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBA7716963.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     LSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7529..8515)
                     /gene="hybA"
                     /locus_tag="J1Z28_002129"
     CDS             complement(7529..8515)
                     /gene="hybA"
                     /locus_tag="J1Z28_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBA7716964.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8518..9636)
                     /gene="hybO"
                     /locus_tag="J1Z28_002130"
     CDS             complement(8518..9636)
                     /gene="hybO"
                     /locus_tag="J1Z28_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBA7716965.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9825..10112)
                     /locus_tag="J1Z28_002131"
     CDS             complement(9825..10112)
                     /locus_tag="J1Z28_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBA7716966.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10231..11118)
                     /locus_tag="J1Z28_002132"
     CDS             complement(10231..11118)
                     /locus_tag="J1Z28_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBA7716967.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11275..12315
                     /gene="gpr"
                     /locus_tag="J1Z28_002133"
     CDS             11275..12315
                     /gene="gpr"
                     /locus_tag="J1Z28_002133"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBA7716968.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12355..12849)
                     /locus_tag="J1Z28_002134"
     CDS             complement(12355..12849)
                     /locus_tag="J1Z28_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBA7716969.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13040..13924
                     /gene="yghA"
                     /locus_tag="J1Z28_002135"
     CDS             13040..13924
                     /gene="yghA"
                     /locus_tag="J1Z28_002135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBA7716970.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13963..14388)
                     /gene="exbD"
                     /locus_tag="J1Z28_002136"
     CDS             complement(13963..14388)
                     /gene="exbD"
                     /locus_tag="J1Z28_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBA7716971.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14395..15129)
                     /locus_tag="J1Z28_002137"
     CDS             complement(14395..15129)
                     /locus_tag="J1Z28_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbB"
                     /protein_id="HBA7716972.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15381..16568
                     /gene="metC"
                     /locus_tag="J1Z28_002138"
     CDS             15381..16568
                     /gene="metC"
                     /locus_tag="J1Z28_002138"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBA7716973.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGKIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16708..17367
                     /locus_tag="J1Z28_002139"
     CDS             16708..17367
                     /locus_tag="J1Z28_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7716974.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTITILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17407..18306)
                     /locus_tag="J1Z28_002140"
     CDS             complement(17407..18306)
                     /locus_tag="J1Z28_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003024513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7716975.1"
                     /translation="MKREEICRLLADKVNNLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18500..19663
                     /gene="yqhD"
                     /locus_tag="J1Z28_002141"
     CDS             18500..19663
                     /gene="yqhD"
                     /locus_tag="J1Z28_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBA7716976.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19768..20595
                     /gene="dkgA"
                     /locus_tag="J1Z28_002142"
     CDS             19768..20595
                     /gene="dkgA"
                     /locus_tag="J1Z28_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBA7716977.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGNALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20795..21721
                     /locus_tag="J1Z28_002143"
     CDS             20795..21721
                     /locus_tag="J1Z28_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBA7716978.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAIVRFRIFQDDKEKTSQTLKMVAESGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21772..22029
                     /gene="yqhH"
                     /locus_tag="J1Z28_002144"
     CDS             21772..22029
                     /gene="yqhH"
                     /locus_tag="J1Z28_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBA7716979.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22071..24290)
                     /locus_tag="J1Z28_002145"
     CDS             complement(22071..24290)
                     /locus_tag="J1Z28_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBA7716980.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMNTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRLQNAGAKKRPKAAVGR"
     gene            24542..25291
                     /locus_tag="J1Z28_002146"
     CDS             24542..25291
                     /locus_tag="J1Z28_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBA7716981.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIRLQENAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
BASE COUNT         6334 a         6727 c         6343 g         5957 t
ORIGIN      
        1 atcagtacgg ggttgctaca acatttatta ctctttcacc acaattaacg gttcaatatc
       61 actctctttt ttgatgacca gtgattcatc accgcgcaaa cacgtcccac cgtagttgcc
      121 gccaacggtg aaagtacata cctgaatgta tttaccgtcc actttcggca aacaccacag
      181 ttgctgatag atgtttttct gctcggcaaa tttaccgcta gtctgatcga gcacctcttc
      241 atgatggctg acgaggtcaa tgttgctacc acagcgcccg gcaatcggtt taacggcata
      301 accggttttc accagctcat cattcacggt gaagtcagta tccaacagat aacgatggtg
      361 cgggaacaga gaccagagga tcggcagaat cgctttgtta ccggggatca ccgtccatag
      421 cggctcaaaa accaacactt ccgggcgcag caatacgtcg ataagacgca cttcattttg
      481 tggatgcccg gtacggatcg gcactgcggc aaattcacgg tcgctcactt cgcggatttg
      541 atcaaatgcc gtttcccacg cccaggtttt ccatacgcag ttaaccagtc gcccttcccc
      601 atcaatcagt tgcccggcag catcccagcc cagttcatcc aacccacgca agatacgcgt
      661 ttcaaagccc gcctggtgca gcgcctgctc cataaactgc gcgtgatagt tttcctcgat
      721 atctttgtcc tgcatgatat ggacaaacgg acgtgcacga ctgtgtttcc acgcgccagc
      781 cagttcgtta atcagtcctt ccgcaggatt gaagccgttg cctttatagc cctgctccgc
      841 ccaacgttcg aggatcaagc ccgcttcggt atgacaggag gcagaatcgg cgttgtattc
      901 gtagactttc aggccacgtt catccatgca gaaatccata cgaccagtga tcatatgatg
      961 acggcgacgc tgccaggaga gacgcaaacg tggccagagg attttcggaa tgtcaaacag
     1021 cgccagcagg ttgtcatctt tcagcacctt gtcggtggcg tgaagataca tcaggtgcag
     1081 ctcgttggtg gctttaatca gctcctgctc ggcactctcg gtaatggtgt agtaatgata
     1141 cggatcctga ttgatcacct ggccgttggc ctgtacatag gcgttttgca gcggatcttt
     1201 ttcatccagc catttaccgt caaactggcc tttgttttcc agacgcgccc cgctgatttt
     1261 cagcagttcg cctgcaattt ccggctgtgg caagctgtat tcggtatctt ccgtctggat
     1321 catccagccc agaatagtgg tgtcatcgaa ggtgtctttc agggtatagc cgccgttttc
     1381 gaccaccatc tccagctcgc gtgtccactg ttgcccttgc ggcaacgggg tatgaatcac
     1441 gttctgttcc gcaatacgga ctttgttgcc atgcaattgg gtaatgatgg cgacatggcc
     1501 agtgtcttta aattcaccgc ctttatccca gataagaagc gcccccgcta ccggtgcacg
     1561 cggcgaaccg ttaggaaatg cctgcaatgg caggatgttg tcattgacca cttcacgcag
     1621 gaagcgcagt gagaaaatct cccatgccat acccacgtca gtgaagacta caccgtagtt
     1681 cagaaagaga aaacggcgag caaattcaac gcattgccat ttgtggccca tatattcatc
     1741 gtcgatatag ctacggaata cggcgtcatc ttcgtattcc tgcggatcga gagaactgta
     1801 atctgaagag tagattgcta ccccacctgg ggcgtagccc aataatgtcc cgaacggggc
     1861 atcctggctg gtcgttcctt tgctcatcac tttaccttta acaatacaac ctaagcagtt
     1921 ggcgtaattg ttgtgctctg attacctgct cagcgacatt aaccggacag aggtcagact
     1981 aatcatacac ctcatcgcag cggctggcgc aaagggttaa aaaattcaca ttctgtacag
     2041 caagtgacct gctacactgc ttcaacacta ccactcagaa ggcaactcac tatgacagac
     2101 aatacttatc agcccgcgaa agtctggacg tgggataaat ccgctggcgg cgcgttcgcc
     2161 agtatcaatc gcccggtttc tggtccgacg catgaaaaaa cgctgccggt gggcaaacat
     2221 ccattgcaac tttattcgct gggaacgccg aacggtcaga aagtaacgat tatgcttgag
     2281 gagctgctgg cgctgggcgt tactggtgca gagtatgacg cctggctgat acgtattggc
     2341 gatggcgatc aattctccag cggctttgtc gaagtgaacc caaattcgaa gatcccggca
     2401 ttgcgcgatc atacccataa tccgccgatc cgcgtgtttg agtctggatc gattctgctt
     2461 tatctggcag agaaatttgg ctacttcttg ccgcaggatc tggcgaaacg tactgaagca
     2521 atgagctggc tgttctggtt acagggcgcg gcaccgttcc tcggcggtgg ttttggtcac
     2581 ttttaccatt acgcaccggt gaaaattgag tacgccatca accgctttac tatggaagcc
     2641 aagcgccttc tcgacgtgct ggataagcaa ctggcgcagc ataagtttgt tgcgggcgat
     2701 gagtacacca ttgcggatat ggcgatttgg ccatggtttg gcaatgtggt gttaggcggc
     2761 gtatatgatg ccgcagagtt tcttgatgcg ggcagttata agcatgtaca acgctgggcg
     2821 aaagaagtgg gcgaacgtcc ggcagtgaag cgtgggcgta ttgttaaccg catcaacgga
     2881 ccgctgaacg agcagttgca tgaacgccat gacgccagtg atttcgagac gaatacggaa
     2941 gataagcgtc aggggtaatg gttagcgttc gtcgcagcaa gccatccaga ccaatgtagg
     3001 ccggataagg cgttcacgcc gcatccggca atcgagcaca atgcctgatg cgacgctgcc
     3061 gcgtcttatc aggcctacaa aagctaatca tcacgcgctg gtaatgtcgt aatccatttg
     3121 gcgcagtgcg tctaatgtgg ctttggcttc gtcttcatcg ataatgctca tggcaaatcc
     3181 gacgtgtacc agtacccact ggcccagcag atcggcaggg ttaccttcac aaatcagggc
     3241 gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagctggt gaatatcttc
     3301 accgacagcc agcacctggc ctggaacgcc aatacacatt gttaactccg gttattcaac
     3361 ttcaatactt ttgacgatta gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca
     3421 gatcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat cccaacacca
     3481 ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgctaccg ttccgtggca
     3541 gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagtg caccaatttc
     3601 cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt ggataatttc
     3661 aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcgg ccaaatagca
     3721 gggcacgacg ccctgcgtgt ggtacatccg gattggtgac tggcagcgaa aggatcattc
     3781 gggcgcagtc atccgtcagc cgttggccct cttcaatcga catgctgtgc gaaagcggcg
     3841 acatcagcga gcaggagagg tattgcgaaa caccatccag ttcaccaaca gtaaaggtca
     3901 tagtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc ggccagagtt
     3961 gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca caccccgtcc
     4021 actgaccttc gaacagcgta aagtcagaaa catacaccgg cattgaagga tgcagaaaag
     4081 agagatcatg catcgaacgc cgggcaattt cttcaaaagc tgcctgtact tgcaccttcg
     4141 gggaggtctg gaaacctgct atctcttcag tcatgagtcg cctcccgtgg gatagcttcc
     4201 acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat catcgcttcg
     4261 accgtcggcg tcaaaccgat gtgcggctcc agtgattccg ggatcacgcc gaccagggtc
     4321 agctttttcg gaaactcgcc ggtgaagcgc agggccgaca atacgtcggc caggccaagc
     4381 tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg caggatcatc
     4441 atcgttcccg gcgcgttctt tttcgagaca atggcatccg caataatcag atgatcgcga
     4501 tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat ctcaacataa
     4561 tccggcagaa tgtatcgttg ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg
     4621 gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc ttcactgaaa
     4681 ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac gggtcgaagg
     4741 agtgaatggt acgcaccact tccagcggtt tattcggatc ggcaaccggt gtacccacca
     4801 gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cgggccagag ttccaggttg
     4861 atggaacaac tgcctggtag ttgctgatga taccgtcttt gatcaccatc cagtgagaga
     4921 gcataccgcg cggtgcttcg aggaagccta cgcctttgaa ctcacccgtt gccggaatgt
     4981 tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt
     5041 gattttgcag gatatcctgc aattcacagc agtgaacggt acgaccaata atacggccca
     5101 gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga taaatcgcaa
     5161 cgatttcatt cagtttgttc tgggtagact cgcgtcctgc cgccagtttc accagcatgt
     5221 tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc acccaggaat
     5281 atttaccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc cacggtgcct
     5341 gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatccct ttgatcaggt
     5401 attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc tcaatgtagc
     5461 cgcccgggaa caggaagctg ccatttttac tgtcggtcgg gaattccggc acgctcaggt
     5521 agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcagcg ataactgcag
     5581 tatcaacctt gtaaacctgc tcaacaaagt cgctcagctt gtcgatgaag gacttgatgt
     5641 acatcaggcg ctcaaggttc agcacaccca gaccgtcgag gttgatcggg ttcgcgacac
     5701 cacctaccgc caggttctga atatgcggcg ttttaccgcc cagcagcgcc acaacgcggt
     5761 tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc aggttcactt
     5821 ccggcggcag tttcattgcc gggtgacccc agtagccatt ggcgaaaata cccaactgac
     5881 cgctggcaac cagatctttg atcttgttct gaactttggt gaactcttcc gggctgttca
     5941 gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggctggatca gcttgcagtg
     6001 cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaaca atatggtcat
     6061 gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc ggaacgtcga
     6121 tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta gtacatacgc
     6181 cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc acgatctctt
     6241 ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca ttttcgattt
     6301 cgcaatcgat gcgtaaatgc ccttcaatac gggttaccgg atcaatagta attctctggc
     6361 tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag gaagtatcgg
     6421 cagtagacga atgagtacga tataagcgca aatctcaata gccacaaaac caatagaaat
     6481 caacagttct tcccaggtcg ggaagtaggc gtaaccgcca cccgggttga atgccaccag
     6541 cgaataggtc agacgccagg ttgcacaacc taacagtgca ctcagtgctg acaggaacag
     6601 catgcgggaa tcattacgca acttcgccac acgcagaacg accagcggga agagcatcag
     6661 caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta acgacagctt
     6721 gtcgcgatag ataagctcgc caaagcgcag cacgatgaaa atcgccagca acacactgat
     6781 ggtattggtc agcttaacga acagactctt ttcatccgga ccgttgccac gcagacccgc
     6841 ctgcaccaac gaaccttcaa agatgacaat cgagaagccc atgatgaacg ccgtcagcag
     6901 cgagaacagc ggcaacattt catagctttg ccacagcgga tgcaccttgt agcccgccga
     6961 gatcatcagc gatcccattg aagactggtg catagttggc agcagcgcac caagcgcgat
     7021 gataaagaac atcaccttgt tcagacgttt gagtgacagc ttccagccca gacgttcaaa
     7081 caatgccgga gcaaactcca gtgccatcac accgatgtag atggtcatac agaccgccgt
     7141 ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg gcaggttcca
     7201 gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gaataaccaa acagactcgc
     7261 caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt atactgccca
     7321 cgccagcgcc cagccgccac aggcaaagcc ggtaccgatc agcaggtcaa acgcgatcca
     7381 cacgccccac gggaagccgc cgttcaggtc ggagacagag cccagaccga acaccagacg
     7441 cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga ccggtttact
     7501 gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct ccgtcgtgat
     7561 ggtcgttttt agtgttgcga cgaaccagca cggttaagcc cgccagcaca gccagtggta
     7621 gcatcatgcc tttatacagg gtgtgttgga catgttcgga acgcgcacca gtagaaagat
     7681 cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc agaacctgag
     7741 taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc gtatgcaggt
     7801 aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgccc ggcttcagcg
     7861 ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accaaaaatc accgcgcccg
     7921 ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc acacctttct
     7981 ggttgcacaa ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg tacttcggca
     8041 cgttgtatgg gcaggcgacc atgcagtaac ggcagccggt gcacacatct ttgtcgtaat
     8101 ggacaatgcc ggttttcgga tcttttttca gcgcagagac cgggcacaca gagacacagt
     8161 tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccattc tcctcctggt
     8221 ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta tacggcgaca
     8281 gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg aagttgatat
     8341 cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc
     8401 ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca gacggcagcg
     8461 cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg ttcacggtta
     8521 ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac gcaccgccat
     8581 cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag cgcctgccga
     8641 gacgttgccg ccttctttag cgttaacgtc cggtttctgt gaacgcggag tctggttttc
     8701 gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt tacagccata
     8761 gcacgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt gcagcgttga
     8821 gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc agccttcgcg
     8881 gtgaccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc
     8941 acagtgttcg tgaatcagac gaccataggc gaaagtcgga cggtttttgt catccagttt
     9001 cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcc aggaagttgt gcgggttcgg
     9061 cgggcagcca ggaatattga taacggtttt gcccggtaga acttcttgca ggctgactgc
     9121 gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg aaccgatagc
     9181 gataatggct gctgcgcctt ccgccgcttt gcggatgtga tccacaatcg gctcaccggc
     9241 aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacca ctaacacata
     9301 ctgccctttg tacttctcga gagcgttatg tttgttctct tcgacctgat gaccgaaggc
     9361 ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt tttctaccgt
     9421 tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgtg cgccaatcca
     9481 gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag cggctttgct
     9541 acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac gacggttaat
     9601 gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg ttgcgaagac
     9661 ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc catttaccac
     9721 acatttatta tggttatcga tatcatgata ctgcggcggg gcactaaaac gaagggaaga
     9781 agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt ttctaggcga
     9841 taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa gaaatccatt
     9901 accatctctt tatccagtcc ataacggcgc gtcagaatac ccgcttccca ggcgctaagc
     9961 tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa accgcgttta
    10021 taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc ttttaatccc
    10081 gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta tatattgtca
    10141 tatatagcga tattttaggc aaagaacgac atttgcgcac gctatttttt catgcttgtt
    10201 taagacgtaa tcctgtaaga gataaaccca tcaggagaga tatttatcga accatttcag
    10261 cgtcctttgc caggcaagat cggcggcaga tttgtcataa cggggcgtgg aatcattatg
    10321 gaatccgtga ttaacccccg gatatatata cgcctcatag accttattat tggctttcaa
    10381 cgccgcctcg taagcgggcc agccctcgtt gattcggcta tccagttcag cgtagtggag
    10441 cagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgcc cataaaacgg
    10501 caccgcgcag gccagttccg gatacgcgac tgctgccgcg ttcgatacgc ccccgccata
    10561 gcaaaatccg ctaataccca ctttgcctgt cgcttgcgga tagcgttgca taaactcaat
    10621 tgcggcaaag aaatcattca tcagtttggt tggatcaacc tgctgttgca gctcacgacc
    10681 tttatcatca tttcccggat aacctccaac ggaacttaag ccgtcaggtg ccagggcgat
    10741 gtaccccgcc ttcgccactc gccgtgccac atcttcgata tacggattca gtccacgatt
    10801 ctcatgcacc accaccacgg ctggcgtttt gccgctcatt tttgcgggct tcaccagata
    10861 accccgtacc tcgccgtgac catttggcga gggatacgtg atgtactcag caacaatttc
    10921 cgggtcggta aactctacct gagtcgccag cgcataattt ggcttgagca aatcaaacag
    10981 cgctaatgcc gtcatcccgc ccaccgcata cttcgccgca aggttgagga actcacgttt
    11041 cgagattttc ccgtgagcgt aatagtcgta gtaatccagc aactcttgtg ggaaatcttt
    11101 ggcggttaaa cgcggcatcg ttgcactcct caactggtgt ttttttaagc aaagcataag
    11161 cgcgtgtttt tgcccagttt ttcgtcactc tgtgagccag actacgggat acgcgctggc
    11221 gaatcgctaa actagaaaca ttgtttcgaa attgaacgat ggaaaggaga ggtcatggtc
    11281 tggttagcga atcccgaacg ttacgggcag atgcagtacc gctattgcgg aaaaagtggt
    11341 ttacgcctgc ccgcgttatc gctcggttta tggcacaatt tcggtcacgt taacgcgctg
    11401 gaatcacagc gtgcgatcct gcgtaaagcg tttgatttag gcattacgca ctttgattta
    11461 gccaacaatt acgggccgcc tccaggaagc gcagaagaga actttggtcg cctgctgcgc
    11521 gaggattttg ccgcttatcg cgatgaactg attatctcta ccaaagccgg atacgatatg
    11581 tggcccggcc catatggctc tggcggctca cgtaaatacc tgctcgccag cctcgaccaa
    11641 agcctgaagc gtatggggct tgagtatgtc gatatctttt actctcatcg cgtcgatgaa
    11701 aatacgccga tggaagaaac cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg
    11761 ctgtatgtcg ggatctcctc ttactcgcca gagcggacgc aaaaaatggt cgagttgctg
    11821 cgcgagtgga aaattccgct gttaattcat caaccttcgt acaatttact gaaccgctgg
    11881 gtggataaaa gcggcctgct ggataccctg caaaataacg gcgtgggctg cattgccttt
    11941 actccactgg ctcagggatt gctgaccgga aaatatctca acggtattcc gcaagattca
    12001 cggatgcatc gtgaagggaa taaagttcgt ggtctgacgc cgaaaatgct caccgaagcc
    12061 aacctcaaca gcctgcgctt attgcatgaa atggcacagc agcgtggaca atcaatggcg
    12121 caaatggcgt taagctggtt gctgaaagat gagcgagtga cgtcggtatt gattggtgcc
    12181 agccgcgcgg agcaacttga ggagaacgtg caggcgctga ataatctgac atttagcacc
    12241 gaggagctgg cgcagattga tcagcatatc gccgatggag agctgaatct gtggcaggcg
    12301 tcttccgata aatgacctgt taataacggg cgcgagaacc gcgcccgaat cagatcagtg
    12361 attatgacga gtcagtcggt caagatagcc catcacaaat gcagagagca caaacgtcag
    12421 atggataatg acgtaccaca tcagtttgtt atcagggaca tttttcgcat ccataaagac
    12481 gcgcagtaag tgaatggaag aaattgccac aatcgacgct gctactttgt ttttcagcga
    12541 cgttgcgtcc attttcccca gccagttcaa cttctctttg ttctcagaga tatcgagttg
    12601 tgagacgaaa ttctcgtaac cggaaaacat caccatcacc agcaaaccgc caaccagcgt
    12661 catatccacc agcgacagca acacgaggat caaatctgat tccgccatcg agaagatatt
    12721 cggcagtacg tgaataatct cctggaagaa cttcagcgcc agggcaacta acgccagcga
    12781 aaggccaaag tacacggggg caagcagcca gcgagaagca tacattgcat tttcaagaaa
    12841 acgttccata cattcctgtc gtgtagttaa aacgggcaac agtatatcgc aattaagtaa
    12901 acagcacgca acagcaaatc aacatatacg ttaaaccttt gtcgcataat tctgagggtt
    12961 agggatcttc ctggttccct tatcaacgca tcgagagcgt ctacccttaa aggacgttga
    13021 acctgaaggg agaaaaacga tgtctcattt aaaagacccg accacgcagt attacactgg
    13081 tgaatatccc aaacagaaac aaccgacgcc aggcatccag gcgaagatga caccggtacc
    13141 ggattgcggc gagaaaacct atgttggtag cggtcgcctg aaagatcgta aagcactggt
    13201 gacagggggc gattccggaa tcggtcgcgc tgccgccatt gcttacgcgc gtgaaggggc
    13261 tgacgtggcg atcagttatc ttcccgtgga agaagaagac gctcaggatg tgaaaaagat
    13321 cattgaagaa tgcggacgca aagccgttct gctgccaggc gatttaagcg atgagaaatt
    13381 cgcccgttcg ctggttcacg aagcacacaa ggcgttaggc gggctggata ttatggcgct
    13441 ggtagccggg aaacaggttg ccattcctga tattgcagac ctcaccagcg aacagtttca
    13501 aaagaccttt gccattaacg ttttcgcgtt gttctggcta acccaggaag cgatccccct
    13561 gctaccgaaa ggtgcaagta tcatcaccac ttcctcaatc caggcatacc agccaagccc
    13621 gcatttactg gactatgcgg ctacgaaggc ggcaattctg aactatagcc gtggcctggc
    13681 aaaacaggtc gcggagaaag gtattcgggt gaatattgtc gcgccaggcc cgatctggac
    13741 agcactgcaa atttctggcg gacaaacgca ggataagatc ccgcagtttg gtcagaaaac
    13801 gccgatgaag cgtgcggggc aaccggcgga gctggcccct gtatatgttt acctggcaag
    13861 tcaggagtcg agctacgtca ccgcagaagt gcacggtgtc tgcggcggcg agcatttagg
    13921 ttaaaaaaat gcccggttgt gaaaagcaac cgggcatcat ttttatttcg ctttcgcagt
    13981 ttcttcgccg accagaccta ttttcaggta acccgcctga tgcagcgtat ccattacctt
    14041 catcaacgtc tcgtaatcga cggttttatc cgctcggaag aagatggtgg tgtctttctt
    14101 gccttcggtt aacgcattca acgccgtaat cattgtttca tcggtgaccg gatcgttacc
    14161 aataaacatc gagttgtctg ctttcaccga cagataaacc ggtttttccg ggcgcggctg
    14221 cggcgtgctg gtagaagcag gcaaattcac cttcacatct accgtcgcta acggtgccgc
    14281 caccataaag ataatcagca aaaccaacat cacgtcgata aacggcgtca cgttgatatc
    14341 atgcatttcg ccgttatcgt cgaggttttc gttaagatgc atcgccatcg gacattatcc
    14401 tgcgcgtaat ttttgtgcga cacgaaccgg atgtgcagcg gcgctggctt ccagatccag
    14461 gtcacggctt tgcagcaaca atacctgcgc tgcaacatca cccagcatcg ctttaaagcc
    14521 gccaatctgg cgtgcaaata cgttatagat aacgaccgca ggaatcgctg ccacgaggcc
    14581 gattgccgtt gctaacagag cttctgcaat acccggcgca acgactgcca ggttagtggt
    14641 ctgcgtttgc gcgataccaa taaagctgtt catgatgccc cagaccgtac caaacagacc
    14701 aacgaacggc gaaatcgcgc cgatggttgc cagatagccg ttaccacggc ccatttgacg
    14761 acctactgcg gcgacccgac gctccagacg gaagctggta cgttctttaa taccttcgtt
    14821 atcgtcgctg ccttctgaca gttccagctc attctgcgct tcattgagca aatgcaggct
    14881 caagcttttg ctaccaaaat cagcggcgat atcgttggcc tggtttaagg aacgcgcttc
    14941 agccagcagt tgctgctcgc gcttaagacg acgcttctga ttgaagaact ccacgctctt
    15001 actgaagaag attgcccagg tgactacgga ggccaaaata agcccaatca tcacgcactt
    15061 aacgacaata tcggcgtgct gatacatacc ccagacggag aggtccgtct gcattaaatt
    15121 attacccact gtgtatctcc aggacgcaag tcacaaaatc tgtgcataat aatatcaaaa
    15181 cgacgtcgaa ttgatagtcg ttctcattac tatttgcata ctgccgtacc tttgctttct
    15241 tttccttgcg tttacgcagt aaaaaagtaa ccagcacgcc atttgcgaaa attttctgct
    15301 ttatgccaat tcttcaggat gcgcccgcga atattcatgc tagtttagac atccagacgt
    15361 ataaaaacag gaatcccgac atggcggaca aaaagcttga tactcaactg gtgaatgcag
    15421 gacgcagcaa aaaatacacg ctcggtgcgg taaatagcgt gattcagcgc gcgtcttcgc
    15481 tggtctttga cagtgtagaa gccaaaaaac acgcgacacg taatcgcgcc aatggcgagt
    15541 tgttctatgg acggcgtgga acgttaaccc atttctcctt acaacaagcg atgtgtgaac
    15601 tggaaggtgg cgcaggctgt gtgctatttc cctgcggggc ggcagcggtt gctaattcca
    15661 ttcttgcttt tgtcgaacag ggcgatcatg tgttgatgac caacaccgcc tatgaaccga
    15721 gtcaggattt ctgtagcaaa atcctcagca aactgggcgt aacgacatcg tggtttgatc
    15781 cgctgattgg tgccgatatc gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg
    15841 aatcgccagg ttccatcacc atggaagtcc acgatgttcc ggcgattgtt gccgccgtac
    15901 gcagtgtggt cccggatgcc atcattatga tcgacaacac ctgggcagcc ggtgtgctgt
    15961 ttaaggcgct ggattttggc atcgatgttt ctattcaggc cgccaccaaa tatctggttg
    16021 ggcattcaga tgcgatgatt ggcactgcgg tgtgcaatgc ccgttgctgg gagcagctgc
    16081 gagagaatgc ctatctgatg ggccagatgg tcgatgccga taccgcctat ataaccagcc
    16141 gtggcctgcg cacattaggt gtgcgtttgc gccaacatca tgaaagcagt ctgaaagtgg
    16201 ccgaatggct ggcagaacat ccgcaagtag cgcgagttaa ccaccctgct ctgcctggca
    16261 gtaaaggaca cgaattctgg aaacgagact ttacaggcag cagcgggcta ttttcctttg
    16321 tgcttaagaa aaaactcaat aatgaagagc tggcgaacta tctggataac ttcagtttat
    16381 tcagcatggc ctactcgtgg ggcgggtatg aatcgttgat cctggcaaat caaccagaac
    16441 atatcgccgc cattcgccca caaggcaaga tcgattttag cgggaccttg attcgcctgc
    16501 atattggtct ggaagatgtc gacgatctga ttgccgatct ggacgccggt tttgcgcgaa
    16561 ttgtataaca ttgccacttt tggacaattt tgcagacatt ttattgtgaa aagtcttaaa
    16621 ttgttgcgtc cgggatcaag gcgtcccgga cgattcagga gtacaatagg cagataaagg
    16681 cttaaacgct gttccacagg aaagtccatg gctgttattc aagatatcat cgctgcgctc
    16741 tggcaacacg actttgccgc gctggcggat cctcatattg ttagcgttgt ttactttgtc
    16801 atgtttgcca cgctgttttt agaaaacggc ctgctgcccg cctcattttt gccaggcgac
    16861 agcttgttga ttctggctgg cgcattgatt gcccaagggg ttatggattt tctgccaaca
    16921 atcacgattc tgactgccgc agccagcctg ggttgttggt tgagttatat tcaggggcgt
    16981 tggctgggta ataccaaaac ggtgaaaggc tggctggcac agcttcctgc taaatatcac
    17041 cagcgcgcca cctgtatgtt tgaccgccac ggtctgctgg cgctgcttgc tggacgtttt
    17101 ctcgcctttg tccgcacact gctgccaacc atggcgggga tttccggact gccaaaccgc
    17161 cgcttccagt ttttcaactg gttaagcggg ttgttatggg tcagcgtagt aaccagtttc
    17221 ggctatgcct taagcatgat tccgttcgtt aaacgccatg aagatcaggt aatgacgttc
    17281 ctgatgatcc tgccaattgc cttgttaacc gctggcttgt taggcacgct gtttgtggtg
    17341 attaaaaaaa aatactgtaa cgcctgacga ttttccccgt tcccggttgc tgtaccggga
    17401 acgtatttaa ttcccctgca tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc
    17461 gaagtaacgt ttaaactcac ggctaaattg cgatgcgctt tcatagccga cgcgcatcgc
    17521 tgctgcgcta gccttcatac cgtcgtggat gatcatcatc cgcgccttat gcagacggta
    17581 attcttcaaa tactgcagcg gcgaggtgct ggtgacagac ttaaaattat ggtggaacgc
    17641 cgatacgctc atgttggctt ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt
    17701 attctcaatc cgtttcagca cgcgactaat caggctgaag tgagtctggc ggctgaccag
    17761 tgccagtaac gcgccgccgc aaggtccggt cagcacgtag tacagaattt cgcggataat
    17821 ctgtttgccg agaatgcgcg catccagcgg tcgctccatc acatccagta accgctctgc
    17881 cgcgcataaa atctcttctg ataacgtggc ggagttaata ccgctggctg ccattgacgg
    17941 ctgaaaatgt tcatcttcgc caatgtccat caacagttcc tgtaactgca aaatatcgac
    18001 attgagacgc aaccctgcca gcggcacctc tgacgtggca taggtttcgc actcaaacgg
    18061 caacggcacc gtcagcagga gatattcatt agcgtcataa cgaaacacgc gttcattgat
    18121 ataaccgatt ttatgtccgg aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt
    18181 gcgtgcgaaa ggcgtctcgc catacaacaa acgcacatcg ggcaacagct ctgacaaact
    18241 attttcttta tttttcaggt tattaacttt atccgccagc aagcggcaaa tctcttcacg
    18301 tttcatatcg cgtaatttct taggaataat gcggcaattt gattgtgcgc aattttgtag
    18361 catttctcca gcactctgga gaaataggca agacattggc agaaatgagc attgagaggt
    18421 acggcgctgg cgaccacaat gaaaaacatc aggcagaccg atctctgccc tcatattgac
    18481 ccagcaaagg gagcaagtaa tgaacaactt taatctgcac accccaaccc gcattctgtt
    18541 tggtaaaggc gcaatcgctg gtttacgcga acaaattcct cacgatgctc gcgtattgat
    18601 tacctacggc ggcggcagcg tgaaaaaaac cggcgttctc gatcaagttc tggatgccct
    18661 gaaaggcatg gacgtactgg aatttggcgg tattgagcca aacccggctt atgaaacgct
    18721 gatgaacgcc gtgaaactgg ttcgcgaaca gaaagtgacg ttcctgctgg cggttggcgg
    18781 cggttctgta ctggacggca ccaaatttat cgccgcagcg gctaactatc cggaaaatat
    18841 cgatccgtgg cacattctgc aaacgggcgg taaagagatt aaaagcgcca tcccgatggg
    18901 ctgtgtgctg acgctgccag caaccggttc agaatccaac gcaggcgcgg tgatctcccg
    18961 taaaaccaca ggcgacaagc aggcgttcca ttctgcccat gttcagcccg tatttgccgt
    19021 gctcgatccg gtttatacct acaccctgcc gccgcgtcag gtggctaacg gcgtagtgga
    19081 cgcctttgta cacaccgtgg aacagtatgt taccaaaccg gttgatgcca aaattcagga
    19141 ccgtttcgca gaaggcattt tgctgacgct gatcgaagat ggtccgaaag ccctgaaaga
    19201 gccagaaaat tacgatgtgc gcgccaacgt catgtgggcg gcgactcagg cgctgaacgg
    19261 tttgatcggc gctggcgtac cgcaggactg ggcaacgcat atgctgggcc acgaactgac
    19321 tgcgatgcac ggtctggatc acgcgcaaac actggctatc gtcctgcctg cactgtggaa
    19381 tgaaaaacgc gataccaagc gcgctaagct gctgcaatat gctgaacgcg tctggaatat
    19441 cactgaaggt tcagacgatg agcgtattga cgccgcgatt gccgcaaccc gcaatttctt
    19501 tgagcaatta ggcgtgccga cccacctctc cgactacggt ctggacggca gctccatccc
    19561 ggctttgctg aaaaaactgg aagagcacgg catgacccaa ctgggcgaaa atcatgacat
    19621 tacgctggat gtcagccgcc gtatatacga agccgcccgc taagcttttt acgcctcaaa
    19681 ctttcgtttt cgggcatttc gtccagactt aagttcacaa cacctcaccg gagcctgctc
    19741 cggtgagttc atataaagga ggaacgtatg gctaatccaa ccgttattaa gctacaggat
    19801 ggcaatgtca tgccccagct gggactgggc gtctggcaag caagtaatga ggaagtaatc
    19861 accgccattc aaaaagcgtt agaagtgggt tatcgctcga ttgataccgc cgcggcctac
    19921 aagaacgaag aaggtgtcgg caatgccctg aaaaatgcct cagtcaacag agaagaactg
    19981 ttcatcacca ctaagctgtg gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac
    20041 agcctgaaaa aactccagct tgattatatc gacctctact taatgcactg gcccgttccc
    20101 gctatcgacc attatgtcga agcatggaaa ggcatgatcg aattgcaaaa agagggatta
    20161 atcaaaagca tcggcgtgtg caacttccag atccatcacc tgcaacgcct gattgatgaa
    20221 actggcgtga cgcctgtgat aaaccagatc gaacttcacc cgctgatgca acaacgccag
    20281 ctacatgcct ggaacgcgac acacaaaatc cagaccgaat cctggagccc attagcgcaa
    20341 ggagggaaag gcgttttcga tcagaaagtc attcgcgatc tggcagataa atacggcaaa
    20401 acacctgcgc agattgttat ccgctggcat ctggatagcg gcctggtggt gatcccaaaa
    20461 tcggtcactc cttcacgcat tgtcgaaaac tttgatgtct gggatttccg tctcgacaaa
    20521 gacgaacttg gcgaaattgc aaaactcgat cagggcaagc gtctcggtcc cgatcctgac
    20581 cagttcggcg gctaacgtgc aaattatccc ggtggcggta atgttccgct accggacttt
    20641 tcagaaatca tttattcccc tcgcgtcccg cccgttgcta ctcttccttg ttcaggaatg
    20701 ccaaatataa ggacatcatc atgcagagcc ggaagctctt aaaagaacaa cccatctata
    20761 tccgggataa atgcaacgga gaggtgaaaa acagatgaaa ataatacttc tgtttttagc
    20821 agccctggca agttttaccg tacacgcaca gcccccctca cagaccgtag aacaaacagt
    20881 ccggcatatt tatcagaact ataaatcaga tgccactgcc ccttattttg gtgaaaccgg
    20941 agagcgggcg ataacatctg cgcgtattca acaggcgctt accctgaacg acaatcttac
    21001 gctgccgggc aatattggct ggctggatta tgatccggtt tgtgattgtc aggattttgg
    21061 cgatctggtg ttagaaagcg tagcgataac ccaaactgac gccgatcatg ccgatgccat
    21121 tgtgcgcttt cgtatctttc aagacgataa agaaaagacc tcgcagacac tgaaaatggt
    21181 ggcggaaagt ggtcgttggg tcattgacga tattgtcagc aatcatggca gcgtcttaca
    21241 agcggttaat agcgagaatg aaaaaacact ggccgctata gcttcgttgc aaaaagaaca
    21301 gccggaagcc tttgttgccg aactctttga acatattgct gattatagct ggccgtggac
    21361 gtgggtggtt tccgactctt accgccaggc ggtcaatgcc ttctataaaa ccaccttcaa
    21421 gacggccaac aatcccgatg aagatatgca aatagaacgg caatttattt acgacaatcc
    21481 gatctgtttt ggcgaagagt cgttattttc acgcgttgat gaaattcgag tcctggagaa
    21541 aaccaccgat tccgcccgca ttcatgttcg ttttacgctg accaatggca acaacgaaga
    21601 gcaagaactg attttacagc ggcgcgaagg taagtgggaa atcgctgatt ttatccgccc
    21661 gaacagcggc agcctactta agcagattga ggcaaaaacc gccgccagat taaaacaatg
    21721 agctgaatta aataacaatt agccggaaca ataaataaaa gggaacacta tatgaaaacg
    21781 attttcaccg tgggagctgt tgttctggca acctgcttgc tcagtggctg cgtcaatgag
    21841 caaaaggtca atcagctggc gagcaatgtg caaacattaa atgccaaaat cgcccggctt
    21901 gagcaggata tgaaagcatt acgcccacaa atctatgctg ccaaatccga agctaacaga
    21961 gccaatacgc gtcttgatgc acaggactat ttcgactgcc tgcgctgctt gcgtatgtac
    22021 gcagaatgat aaaaaaatcc ccggcagcat tctgttaccg gggatatttt ttaacgtcca
    22081 accgccgctt tagggcgttt cttcgcacca gcattctgca ggcgagattg cgtagacgac
    22141 gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca tcggcgtatg tttagtcaac
    22201 gccggacggg tattgcggtt ctgacggcga gcttcacgca tctcttcaat ggttggcgca
    22261 ggcactaagc aatcgcgacg gctgccaatc agatgctttt tgcccatcgc ttccagcgcc
    22321 tggcggatta acggccagtt tgccggatcg tggtaacgca acaacgcttt atgcaggcga
    22381 cgctgcttgt cgcccttcgg tacgaagacg tcttcactct tataaccaat cttcgccagc
    22441 gggtttttgc cggtgtaata catggtggtc gagttagcca gcggcgacgg atagaagttc
    22501 tgcacctggt cgaggcggaa gcgatgcttt ttcagccata gcgccagatt caccatatct
    22561 tcatcacgcg tacccgggtg cgcggagatg aaatacggga tcagatactg ttctttacct
    22621 gcctgtttcg agtaagtatc gaacagctct ttaaagcggt catagctgcc catgcccggc
    22681 ttcatcatct tcgataacgg cccttcttcg gtatgttccg gggcaatctt cagataaccg
    22741 ccaacgtgat gggtcgccag ctctttgata tagcgcggat cttctacggc gatgtcataa
    22801 cgcacaccag aggcaatcag gatcttttta atgcctttca gatcgcgcgc acggcgatag
    22861 aggttgatcg tcggttcgtg gttagtgttc atatgcggac aaatatccgg ataaacgcac
    22921 gacaaacgac ggcaggtttg ctcagcgcgt ggcgatttgc aacgcaacat atacatgttg
    22981 gcagtaggcc caccgagatc ggaaatcacg cccgtaaaac ctggaacagt gtcgcggatc
    23041 gcttcaatct cattaataat cgaatcttcg gaacggctct gaataatgcg cccttcgtgc
    23101 tcggtaatag aacagaaaga gcagccgcca aagcagccac gcataatgtt gaccgagaaa
    23161 cgaatcattt cgtaagccgg aattcgggca ttaccgtaag ccggatgcgg cacgcgcttg
    23221 tacggcagcg caaagacgct atccatctct tcggtagaaa gcggaatagc aggcgggttg
    23281 atccatacat agcggtcgcc atgtttttgc atcaatgcgc gggcgcagcc tgggttagtt
    23341 tcgtggtgca gaatgcgcga agcatgggcg tacagcactt tatcgccctt cactttctcg
    23401 aaagaaggca gcaacacgta ggttttttcc cacggtttcg ggcgtggtgg ctgcacggtt
    23461 acagctttgg cttcctgctt tttcggagct accggtttgt tatccgcgca cggcaaatct
    23521 tcgccatacg gatgcgggat tgggtcgatt ttccctgggg tatcaagacg ggtggaatcc
    23581 acaccgctcc agcctggcaa cgcctctttt acgataatcg cggtattacg cacatcgcgg
    23641 atttcactaa tcggctcgcc catcgccaga cggtgcgcca cttccaccag cggacgctcg
    23701 ccgttaccga acatcagcat gtcagctttg gaatccacca acaccgaacg gcgcacggta
    23761 tccgaccagt aatcataatg cgcggtacgg cgcaggctgg cctcaatacc gccgaggatc
    23821 accggcacat ctttccacgc ctctttacaa cgctgggtat aaaccagtgt ggcgcgatcc
    23881 ggacgcttac ccgcgacgtt atccggtgtg taggcatcgt catgacgtaa acggcgatcg
    23941 gcggtatagc ggttgatcat cgagtccatg ttgccagcag taacaccgaa aaacagattc
    24001 ggtttaccca gacgcataaa gtcgtcttta ctgctccagt ctggctgggc gatgatcccc
    24061 acgcgaaagc cctgcgcttc cagcatacga ccgcaaatcg ccatcccgaa gcttgggtga
    24121 tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc tatcccagcc aagttgatcc
    24181 atctcttcac gtgacatcgg caaaaacggt gccggtccaa aacaggcggc ccagtactgc
    24241 ggccaggaga acaggtcgcg atccggttgg atcagggaga tagagctcat tttgcttcca
    24301 gaaatggtaa aaaaataatc aaaggccggg gattatacgc tgtatcacgc gcggaaatga
    24361 agtgatgttg atgaacggtg gcgaaatacc ctggtgaggt ttttcactgc ccgacttggg
    24421 caagcacatt taaggctggc ggtatcgtgg caactgccgc tactatttat gccgatcagc
    24481 aacgttcaaa tcatattgca cgacgatcgg ctatccgagg acacccacag taaggcaagg
    24541 tatggaacag gttattacaa agcgccgcta ttacgatatt ggtctacaga ttgaagagct
    24601 gctgtattca ggcgtattta aggccggaga acgcttacct tcagaacgtg aactgagcga
    24661 gcggtttaat accagccgaa ctaccattcg tgaagccatc attatgctgg aactgaaagg
    24721 cgtattgaat gtgaagcaag ggtcagggat tttctttgtc gactccaccg ataagctcaa
    24781 ccagaaatca ttaatgccct attcagaaat tggcccgttt gaactgttgc aggcgcgtca
    24841 ggtgattgag agcaatatta ctggttttgc agccagccag atcagcttta acgagttaca
    24901 agagttaaag aaaatcatta ggttgcagga aaacgctatc gctgcggaaa gtgataaatt
    24961 tgaagacctg gatcaccgat ttcatagcat tattgccgaa gcgacgcaaa atcgggtact
    25021 catcaaacaa gccgctgaat tatggcgagc ggtacgtaca gaaaaccccc gctggaagaa
    25081 acttaactac aagtacctgc atgaaaagca tttgcgtctg caatggctgg aagatcaccg
    25141 ggctattttc ctggctctgc aacagaaaga ttcagagctc gccagggaag cgtcctggag
    25201 acatctggaa aacagcaaaa atgaactgat caaaatcttc aaacaagacg cttccatcag
    25261 cgattttgat gactttttct tcgctcgtta accttcatta gtccagagct ggcgggaaaa
    25321 ataacgccgg ctctgccttc cgccctccac tggtacacgc t
//
LOCUS       DADSZD010000060        24833 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.060, whole genome shotgun sequence.
ACCESSION   DADSZD010000060 DADSZD010000000
VERSION     DADSZD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.060"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..429
                     /locus_tag="J1Z28_002147"
     CDS             <1..429
                     /locus_tag="J1Z28_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7716982.1"
                     /translation="LINHFILTKTTFGRKIYITGGNKEAAIYSGINVTRLKIKVFMIT
                     AVLAGISGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGYGTVVGTVIGALTI
                     GVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            451..1413
                     /locus_tag="J1Z28_002148"
     CDS             451..1413
                     /locus_tag="J1Z28_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7716983.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLMEIETFIQRHDMSIREAAQ"
     gene            1410..2456
                     /locus_tag="J1Z28_002149"
     CDS             1410..2456
                     /locus_tag="J1Z28_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBA7716984.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGIVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDNGETLGLHGRLAATPAEEVGYRTERTDDGIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVLRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTP
                     HAAEGLTSHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(2548..3897)
                     /gene="accC"
                     /locus_tag="J1Z28_002150"
     CDS             complement(2548..3897)
                     /gene="accC"
                     /locus_tag="J1Z28_002150"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBA7716985.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(3908..4378)
                     /gene="accB"
                     /locus_tag="J1Z28_002151"
                     /gene_synonym="fabE"
     CDS             complement(3908..4378)
                     /gene="accB"
                     /locus_tag="J1Z28_002151"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBA7716986.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5244..6074)
                     /locus_tag="J1Z28_002152"
     CDS             complement(5244..6074)
                     /locus_tag="J1Z28_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7716987.1"
                     /translation="MKSLFNRLTEKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLEEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEILNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AINNLKQILPEVSAEIVDTGANAGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTYNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(6111..7085)
                     /locus_tag="J1Z28_002153"
     CDS             complement(6111..7085)
                     /locus_tag="J1Z28_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBA7716988.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDITVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEVVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            7237..9177
                     /gene="csrD"
                     /locus_tag="J1Z28_002154"
     CDS             7237..9177
                     /gene="csrD"
                     /locus_tag="J1Z28_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBA7716989.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTYLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9482..10525
                     /locus_tag="J1Z28_002155"
     CDS             9482..10525
                     /locus_tag="J1Z28_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBA7716990.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10591..11694
                     /gene="mreC"
                     /locus_tag="J1Z28_002156"
     CDS             10591..11694
                     /gene="mreC"
                     /locus_tag="J1Z28_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBA7716991.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11694..12182
                     /gene="mreD"
                     /locus_tag="J1Z28_002157"
     CDS             11694..12182
                     /gene="mreD"
                     /locus_tag="J1Z28_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBA7716992.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            12191..12784
                     /gene="yhdE"
                     /locus_tag="J1Z28_002158"
     CDS             12191..12784
                     /gene="yhdE"
                     /locus_tag="J1Z28_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBA7716993.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12774..14243
                     /gene="rng"
                     /locus_tag="J1Z28_002159"
     CDS             12774..14243
                     /gene="rng"
                     /locus_tag="J1Z28_002159"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBA7716994.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            14311..18111
                     /locus_tag="J1Z28_002160"
     CDS             14311..18111
                     /locus_tag="J1Z28_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein"
                     /protein_id="HBA7716995.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLVLDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEITD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTAEGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            18267..19712
                     /gene="tldD"
                     /locus_tag="J1Z28_002161"
     CDS             18267..19712
                     /gene="tldD"
                     /locus_tag="J1Z28_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBA7716996.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGVVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19840..20769)
                     /gene="aaeR"
                     /locus_tag="J1Z28_002162"
     CDS             complement(19840..20769)
                     /gene="aaeR"
                     /locus_tag="J1Z28_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBA7716997.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20952..21155
                     /locus_tag="J1Z28_002163"
     CDS             20952..21155
                     /locus_tag="J1Z28_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBA7716998.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            21163..22095
                     /gene="aaeA"
                     /locus_tag="J1Z28_002164"
     CDS             21163..22095
                     /gene="aaeA"
                     /locus_tag="J1Z28_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBA7716999.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            22101..24068
                     /gene="aaeB"
                     /locus_tag="J1Z28_002165"
     CDS             22101..24068
                     /gene="aaeB"
                     /locus_tag="J1Z28_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBA7717000.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            24160..24432
                     /locus_tag="J1Z28_002166"
     CDS             24160..24432
                     /locus_tag="J1Z28_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717001.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24487..24750)
                     /gene="yhcN"
                     /locus_tag="J1Z28_002167"
     CDS             complement(24487..24750)
                     /gene="yhcN"
                     /locus_tag="J1Z28_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBA7717002.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5899 a         6047 c         6733 g         6154 t
ORIGIN      
        1 ctgataaacc acttcattct tactaaaaca acctttggac gaaaaattta tatcactggt
       61 ggtaataaag aagcagcaat ttattcaggg ataaatgtca cccgcttgaa aattaaagtc
      121 tttatgatta cagccgtgct ggcaggtatc agcgggatga tccttgcctc acgcctctat
      181 tccgggcaac cgaattccgc gttgagctac gaactggatg ctattgccgc agctgtactg
      241 ggaggcacca gcctgaacgg cggctatgga accgtggtgg ggacggtgat cggcgcatta
      301 accatcggcg ttattaataa tggcatgaac ttaatgaatg ttccttattt ttaccagatg
      361 gtggttaaag gattagtcat tcttgtagcc gtttattttg acgtaagaaa taaaagaaaa
      421 cgcagctaat catttcaagg aattaatcat atgagtaagg tcttcacaat tggagaaatt
      481 ctggttgaaa ttatggcgag taaaatcggc cagccatttg atcaaccagg tatctggaac
      541 ggcccttatc ccagtggtgc gcccgctatt tttatcgacc aggtgacacg tcttggcgtc
      601 ccgtgcggca tcattagctg cgtggggaat gatggctttg gtgatattaa tatacatcgt
      661 ctggctgctg acggtgtgga catcaggggg atttcagtct tacctctgga agccaccggt
      721 agtgcctttg ttacttatca taactctggc gatcgcgatt ttatttttaa tatcaagaat
      781 gccgcttgcg gtaaattatc ggcacagcat gttgatgaaa atattctgaa ggattgtacc
      841 cattttcata ttatgggttc atccctgttt tcattccata tggtagacgc agtaaaaaaa
      901 gcagtaacca tcgttaaagc aaatggtggc gttatttctt tcgacccgaa tattcgtaaa
      961 gaaatgcttg atattccaga aatgcgtgac gccctccact ttgttcttga gttaacagat
     1021 atctatatgc ccagcgaggg agaagtgttg ctcttgtcgc cacactcgac gccggagcgt
     1081 gcaatcgctg gttttcttga agaaggtgtt aaagaggtga ttgtgaagcg cggcaatcaa
     1141 ggtgccagtt actactccgc gaatgagcag tttcatgttg agagctatcc ggtggaagaa
     1201 gttgacccga ccggtgcggg agactgcttt ggtggcgcgt ggattgcctg ccgccagtta
     1261 gggtttgacg cccatcgggc attacaatat gccaacgcct gcggtgcact ggcggtaacc
     1321 cgacgtggcc caatggaggg aacgtctcgc cttatggaaa tcgaaacgtt catccagcgc
     1381 catgacatgt ccattcggga agcagcacaa tgaaaaacga aatttggtcg tggattggcg
     1441 atctctcgca ggttgccgga attaaacact atgagttacg tagtggcaga gcaaagggga
     1501 cagaagcgtt tgatgtccgt actggtgcgg gactggcatt tacggtggta aaagatcgag
     1561 cgctggatat tgcatgggcc agctacaagg acactgcgct ctcttttatc acccccaacg
     1621 gcattgtcgc ccccgctttt tttgagtctc agggcaacgg gtttttacgt agcttctacg
     1681 cggggttact gactacctgc ggacttagct acattggcac gccctgtgag gataacggag
     1741 aaacgctggg gttacatggg cgtttggctg cgactccagc agaagaggtg gggtatcgca
     1801 cagaacggac cgacgacggc attgaatttg tcatcaatgg caaagtacgg gaaacacgat
     1861 tgttcggtga aaatctgacg ctggaaagga ccattcgctg ccgttatggc gaaaatgtgc
     1921 tgcgcattga agacaaggtg actaatcatg gtttcacccg ccagccgctg caaattcttt
     1981 atcactttaa ttacggctgg cctttattat cgccacaggc agaaatcttg ctgtcggcta
     2041 aaagcgtaac gccacgaacc ccgcatgcgg cggaaggatt aaccagtcat ctggagattt
     2101 gtaccccaca gcccggtttt gacgagcagg tttactatct cacgttgaat agcgacagtc
     2161 aggggatgag taaagttgcg ctggtgaacg ctgaactggg gtggggaatt tacgagaaat
     2221 tcgacaccat gcagctaccc aactttatcc agtggaaaaa tttaggcgcg ggtgaatatg
     2281 ttatgggact ggaggtcagt aatagttttc ctgacggtcg tgataaagaa cgagcgcagg
     2341 ggcgtctgcc ctttattgag ccaggggaaa caaagaaata ctgctttgag ctgggaattg
     2401 ttgatggcga tgctgaaatt agcgcgttga aagcggaaat tgcaggctac cgttagttaa
     2461 cggcaataaa aacgccgggt agcattaaca ggcccggcgt tgattacgtt ctttgctaag
     2521 cgcgatggaa acaccgcgcg tcacaaatta tttttcctga agaccgagtt ttttctccag
     2581 atagtggatg ttagtgccac catgctggaa gttttcgtca ttcatgatgc ggatctgcag
     2641 atcaacgttg gttttgatac cgtcgatgat cagctcctgc agcgcattct tcatgcgagc
     2701 aatcgccacg tcacggtttt caccgtagca aatcagctta ccgatcattg agtcatagta
     2761 tggcggtacg gtgtagcccg cgtagatatg agactcccaa cgtacgccaa aaccaccagg
     2821 tgcgtggaaa cgggtgattt tgcccggact tggcaggaag gtgttcggat cttcggcgtt
     2881 gatacggcat tccaccgcat ggccgcgaac gtgaacttct tcttgcttga tcgacagcgg
     2941 ttgaccggca gcgatacgca gctgttcttt gatcaggtca acgccggtga tcatttcagt
     3001 aaccgggtgt tctacctgaa tacgggtgtt catttcgatg aaatagaact cgccgttttc
     3061 gaacaggaac tcgaaagtac ctgcaccgcg atagccgata tcaacacacg ctttagcgca
     3121 acgttcgccg atgtagcgac gcagttccgg ggtaatgccc ggagctggtg cttcttcgac
     3181 cactttctgg tggcggcgct gcatggagca gtcacgttcc gccagataga tagcgttgcc
     3241 ctgaccgtca gccagtacct gaatctcgac gtggcgagga ttttccaggt atttctccat
     3301 gtaaaccata tcgttgctga aagcagcttt cgcttccgca cgggtcatgg agatggattg
     3361 tgccagttca gcgtcgccgc gcactacgcg cataccgcga ccgccgccgc cgccggaggc
     3421 tttgataatt accggataac caatgcgttt agcaatggcg cggtttttat ccatatcgtc
     3481 gcccagcggg ccgtcagaac ccggtacgca aggaacgcct gcttttttca tcgccgcgat
     3541 tgcggatact ttgtcgccca tcaggcgaat ggtttctgct ttcgggccga tgaagataaa
     3601 gccggagcgt tcaacctgct cggcaaaatt ggcgttctcg gagaggaagc cgtaacccgg
     3661 atggattgct actgcgccgg tgatttcagc ggcgctgatg attgccggga tgttcagata
     3721 actttttact gacggagcag ggccgataca gaccgtttca tctgccagta atacgtgttt
     3781 tagatcgcga tccgcactgg agtgcacagc gacagtcttg atgcccagtt ctttacaggc
     3841 acgaagaata cgcaatgcaa tctcgccgcg gttggcaata acaattttat ccagcatgtt
     3901 cgcctcgtta ctcgatgacg accagcggct cgtcaaattc taccggttgt ccactttcga
     3961 ccagaattgc tttcacggta ccggatttgt ccgcttcgat ctggttcatc attttcatgg
     4021 cttcaacgat gcacagggta tcgcccacgt tgactttctg acccacttcg atgaacgctt
     4081 ttgcgtccgg gcttggggtg cggtagaaag taccaaccat cggggaacgt acgatgtgac
     4141 cactgatttc cgctgctgct ggcgcttcca tggaaggaac ggtcgccgga gcggctgcgt
     4201 tagattgagc tggttgctgc atcataggtg cagcgtaagc ttgttgcatc acagggaaac
     4261 ttgcggcagg agctgcacgg ctaatgcgta ctgactcttc gccttcagaa atttccagtt
     4321 cggagatgcc tgattcttca accagctcga tcagtttttt aatcttacga atatccatga
     4381 gtgggttccg tactctttgt ttagtgtgat tgtgacaggc gtttcaccgc cgtctgtaaa
     4441 gcgtatgccc cgacagctgt atgcatagcg ataaattcca gcaggccggg acgccggtct
     4501 attttgcccc agagtgtcaa tgttagactt gacggacatt gtgcagcgat ctgctgctat
     4561 cctccggcaa aaaacaaaat ataccttcgt tgcgccaatg tcaccttttc cgcagcgcaa
     4621 aaactgcgtc agggaggacg aggtcgcaca ttataacgat ttcgtagcaa ttggcagcta
     4681 aatactggtc ttatcaggga agataatcaa cagctaacat gtaaataacc ttcaacaccg
     4741 tgtaatttgc aacaagccgc acaattcacg aaattagcgc catcatcgac ggaacttctt
     4801 ataacgtaag gtgaaaagct gtaaagccag ccctgcgaag atggggggct gcaatgagat
     4861 aatcttcaca gaccacaaat aatgtaggga tgccaggatc gcgacaagat agacgacatt
     4921 acgcagcggt tgctggggat tgcccaattt tcactgcatc gtctggatca acgtgaatgc
     4981 taaagcacgc aaaatcaacc gcctgataat gctcagtatg aaataggttt aagtggttaa
     5041 tttgtaatca aataaaatca gattattccc cccattttgc agaaggtaaa ctgttttgtc
     5101 aggcggcagt gcggctatgt gatgcattca gaatttgccg cgtgacaata aaaaagccga
     5161 tttggtggca aaatcggctt cggtgagttt gcagcattca tcacatactc cgacaagctt
     5221 tttcaagcat gacggaggtt gcgttaatgc aaggtttcta cccagggata aaagtgaccg
     5281 ttacggcgga tgaatatctg tgttgtcaga ggatggtcat cctcacggaa gcattgttct
     5341 gttaactgtc gtaatgcgct gagatttgct ggctgatcat tgtaggtgaa aaacagtttt
     5401 cctctggagg gaacagtcac taacaggtcg ccgcattcag gatcgggtga ttgcacccaa
     5461 agatcgtcaa tgagcatcag actgggctca taagtacccc ctgcggagat aacggcaact
     5521 tttccagcgt ttgcgcctgt atcaacgatt tcagccgaaa cttccggcaa tatttgcttc
     5581 aaattattga tggcttgttc gcggtcaatg gtcacttcgt agcgcatcag atagcgcatg
     5641 gtttcgctac catcttcagc aaacaagata attaactcat cgttaaagtc ttcccaggca
     5701 aaatccagcg aggcgttgag gatctctgga tcagattcat actggctaag ctgttggcga
     5761 acggtatcca gccaggcttt ttctttaatc accggaacaa tacagcgccc ggtcggcagc
     5821 atttctgcaa gctgtttcag catatctgca gccacttctt ccagcgggtc tggtgcggct
     5881 tgataacgtt tgtaggcatc atcaagagtg atttccaccg tttggtgacc cgtatcaatg
     5941 cgcatcaatt tatggtcagt cgaaatcatg actttccagt ttggatgatg ttgtacaact
     6001 tcctgaccaa ggtgttcgac gaaagctgtc cgactaaccg ctttttccgt taaccgattg
     6061 aataatgatt tcaaaatgag ttccctctct tattaatcct tgctaaatgg ttagttaacc
     6121 ttcaccagcg tgcgaccctg gatctggtta ttaatgatgg cctcggcaaa cttcggtgcc
     6181 tctgccagag atatctcttt tgccgcctgg gtatagaatg attccggtaa atcggcgacc
     6241 agtcgctgcc aggcttgtgc gcggcgtgct ggtggcgtca ttactgaatc caccccttgc
     6301 aaacggacat tacgcagaat aaatggcatg accgtggttg gcagagtaaa accacccgcc
     6361 agaccacagg ccgccacgca gccgccgtaa ttcatttgcg ccagcacttt tgccagcact
     6421 ttgtcgccaa cggtgtcaat tgccccagcc cagacttgtt tttccagagg acgggattca
     6481 gcaaactcat cgcgaggaag aatacggctg gcacctaaac ttttcagata ttcatgggta
     6541 ctttcgcgac cggaaacggc aacgacctga taacccaact tatgcagcag cgccacggcg
     6601 gtactgccga cgccaccact ggcacccgtc acgacaacct ccccatcctg cgggcgaaca
     6661 ccggcatctt ccagcgccat cacacacagc atggcggtaa aaccggcagt accgataatc
     6721 attgctttac gcgcgtccag cccttgcggc atggcaacca gccagtcacc tttcactcgc
     6781 gcctgttccg ccagcccacc ccagtggttt tcaccaacgc cccagccagt gagtaacacc
     6841 tcctgaccgg catgaaaacg cggatcttcg ctggtgcgta cagttccggc aaaatcaatc
     6901 ccaggaatca tcggaaaatt acggatgatt tttcccttac cggtaatcgc cagcgcatct
     6961 ttgtagttca ggctcgacca gtgaacatca accgtgatat cgccttccgg caggcgactt
     7021 tcgtccagag tctgtactga tgcgagagtt ttgccgtcct gctgttctaa aagtaacgcc
     7081 tgcataagtg gtcctcatgt gcatgatgga ttggaaaata atttctgaag actatactcg
     7141 ctaatggaaa agcaaagccg atgaagcgca ataaattgcg agataaatct gatttgctag
     7201 tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac gaaattttcg
     7261 gcctttgtca cgctgctcac cgggttaaca atttttgtga ctttgctggg ctgttcgcta
     7321 agtttctaca acgccattca gtataagttt agtcatcgtg ttcaggcggt ggcgacggcg
     7381 attgatacct accttgtgtc gaatgacttc agcacattaa ggccacaaat taccgaatta
     7441 atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg gtgataagca ggtttatacc
     7501 ctggccagaa atggtagtta tcgtccggtt ggcaccaacg atctgtttcg tgaactgagc
     7561 gttccgttga taaagcatcc ggggatgtcg ctgcgtctgg tttatcagga tccgatgggc
     7621 aactatttcc attctttaat gactaccgcg ccgctcacgg gggcgattgg ctttatcatt
     7681 cttatgctct tcctggcggt acgctggtta caacggcaac ttgccgggca agaattgctg
     7741 gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt gggaaccatc
     7801 tatgaatggc cgcccagaac cagcagtgcg ctggatacgc tgctttgtga aattcagaac
     7861 gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc ccaggacatg
     7921 aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt actggaagat
     7981 caagagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga tttcaatatg
     8041 ttgagtgata cctgggggca cagccaggtt gaagaacagt tcttctctct gacgaatctg
     8101 ctatcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca ccgcagtgat
     8161 tttgctgcgt tgttaccgca ccggacgtta aaagaggcag agagcatcgc cagtcagtta
     8221 atcaaagccg tcgatacctt gccgaacaat aaaatgctcg atcgcgacga tatgatccac
     8281 attggtatct gtgcctggcg tagtggtcag gataccgagc aggtaatgga acatgcagag
     8341 tctgccacgc gtaatgcggg attgcagggc ggcaatagct gggctattta cgatgactcg
     8401 ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca aatgctcagt
     8461 cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggcca ggttcatcat
     8521 cgtgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc agagtatatg
     8581 ccgatggtct tgcagtttgg tttatcggaa gagtatgacc gcctgcaaat cagccgtctt
     8641 attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac cgttgagtcg
     8701 ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg tgaaaaatcg
     8761 caacgaagac gcataattat tgaacttgca gaggccgatg ttggtcaaca tatcagtcgc
     8821 ttacaacctg ttattcgttt agtgaatgct ttaggggtac gggtagccgt caaccaggct
     8881 ggtttgacgc tggtaagcac cagttggatc aaagaactta atgttgagtt actcaagctc
     8941 catccggggc tggtcagaaa cattgagaag cgaacggaga accagctgct ggttcaaagc
     9001 ctggtggaag cctgctccgg gaccagcacc caggtttacg ccaccggcgt acgttcgcga
     9061 agcgagtggc agaccctgat tcagcgcggt gttacaggtg ggcaagggga ttttttcgcg
     9121 tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc ggtttaacct
     9181 gccgtttaat ccgttttcac gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt
     9241 ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg ttgcaaacag
     9301 gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt tgtaacaaag
     9361 gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg
     9421 tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat tatcccttag
     9481 tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc tgggtactgc
     9541 gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt ccgtggtggc
     9601 cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa
     9661 gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga aagacggcgt
     9721 tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag
     9781 caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtgccggttg gcgcgaccca
     9841 ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct
     9901 gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg
     9961 ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct ccttgaacgg
    10021 cgtggtttac tcctcttccg tgcgcattgg tggtgaccgt ttcgacgaag ctatcatcaa
    10081 ctatgtacgt cgtaattacg gttctctgat cggtgaagcc accgcagaac gtatcaagca
    10141 cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa
    10201 ccttgcagaa ggtgttccac gcggttttac cctgaactcc aacgaaatcc tcgaagcact
    10261 gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc
    10321 ggaactggct tccgatatct ccgagcgcgg catggtgctc accggtggtg gcgcactgct
    10381 gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg ttgctgaaga
    10441 cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg
    10501 cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaagtgtct gtttaccctg
    10561 cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg tggcccgtcg
    10621 ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat tgccgacagt
    10681 cgcctgggga cattcagtca aatccgtact tatatggata ccgccgtcag tcctttctac
    10741 tttgtttcca atgctcctcg tgaattgctg gatggcgtat cgcagacgct ggcctcgcgt
    10801 gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa
    10861 ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct actgggttcc
    10921 ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt taacgatcct
    10981 tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga aggccagccg
    11041 gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact gaccagccgc
    11101 gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg caacgatatc
    11161 cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca tctgccagcg
    11221 aatacggata ttcgtgttgg tgatgtgctg gtgacttccg gtctgggcgg tcgtttcccg
    11281 gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg cgcttatact
    11341 gtgattcagg cgcgtccgac tgcaggactg caacgtttgc gttatctgct gctgctgtgg
    11401 ggcgcggatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg tgttgctaat
    11461 gaacgtttga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat ggggccaaag
    11521 ttacctgaac cggcaacggg gatcactcag ccgactccgc agcaaccggc gacaggaaat
    11581 gcagttactg cgcctgctgc gcccacacag cccgctgcta atcgctctcc acaaagggct
    11641 acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggaggaca atagtggcga
    11701 gctatcgtag ccagggacgc tgggtaatct ggctctcttt cctcattgcg ctgttgctgc
    11761 agatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg ttactcatct
    11821 tgttgtattg gatcctggcc ttgccccatc gcgtaaatgt gggcacaggt tttgtgatgg
    11881 gtgccatact ggatctgatc agcggctcga cgcttggcgt acgcgtattg gcgatgagca
    11941 tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca ttatggcagc
    12001 aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc tgggcagagt
    12061 ttttagtgat taacgtctct ttcagaccgg aagtattctg gagtagtgta gtcaatggcg
    12121 ttctctggcc gtggattttc ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat
    12181 aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc aggagttact
    12241 tgcgcaactt ggcgtgacct ttgaacgtat tgttacgggc attgaggagc agcgtcagcc
    12301 gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc gagaaagcac aggcgggtgt
    12361 cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggat actatcgtta tcctgaacgg
    12421 agaagtgctc gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc
    12481 gggtcagacc catcaggtga tgacggcagt ggcgttggct gacagccagc acattctcga
    12541 ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca tcgcgggcta
    12601 tgtcgccagc ggcgaaccgt tagataaagc aggtgcatac ggtattcagg ggctgggtgg
    12661 ctgttttgtc aggaagataa atggcagctt tcacgccgta gtcggcttac cgctggttga
    12721 aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata aacatgacgg
    12781 ctgaattgtt ggtaaacgta acgccttcgg aaactcgagt ggcgtatatt gatggcggta
    12841 ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca
    12901 agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat attgggctgg
    12961 ataaagccgc gtttcttcat gcatctgaca tcatgccgca caccgaatgt gtggcgggtg
    13021 aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggtccgtcag gggcaagatc
    13081 tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg accaccgata
    13141 tcacgctgcc ttctcgctat ctggtgttta tgccaggggc ttctcacgtt ggggtatccc
    13201 aacgaattga aagcgaatca gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg
    13261 acgagcaggg cgggtttatc atccgtactg cagcggaagg ggttggcgag gctgaactgg
    13321 cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt aaaaaacgcc
    13381 cgcagacccg ttatcagctg tacggagaac tggcgctggc gcagcgtgtt ctgcgtgatt
    13441 tcgccgacgc cgaactcgac cgcattcgcg ttgactcacg cctgacttac gaagcattgc
    13501 tggagtttac ctcggagtac attcccgaga tgaccagcaa gctggagcat tacaccggac
    13561 gccagccgat tttcgatctc tttgatgtcg aaaacgaaat tcagcgagcg ctggaacgca
    13621 aagtagaact gaaatccggt ggttatctga ttatcgacca gaccgaagcg atgaccaccg
    13681 tggacatcaa taccggggcg tttgtcggtc atcgcaatct ggacgacacc attttcaata
    13741 ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgcaat ctgggcggga
    13801 ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgccgc gtgctgcatt
    13861 cgctggagca ggcgttgagc aaagaccggg tgaaaaccag cgttaatggt ttttcggcgc
    13921 tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac gtactgtgta
    13981 acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg
    14041 aaatcatgcg cgagattgtt cgtgtacacc atgcttacga ctccgaccgt ttcctggtct
    14101 atgcttctcc ggcagtagcc gaagccttga aaggcgaaga gtcacactcg ctggcggaag
    14161 tggaaatttt cgttggcaaa caggttaaag tacaaattga accgctctat aaccaggagc
    14221 agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa gggtttttga
    14281 gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat tttactgctt
    14341 actggagccg cgctcgttgt gatcgctgcc ctgctggtta gcggcctgcg tattgcttta
    14401 ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc gactggcatg
    14461 ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggtccgac gcttgaagcg
    14521 cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg cgttactctg
    14581 gtgctggatg tctggcagag cctgttacat atgcgctggc agtttcgcga cctcactttc
    14641 tggcagctgc gttttcgcac caacactcct atcaccagcg gcggtggtaa tgatagcctg
    14701 gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga tctccgcgac
    14761 agtgaagtca gtttcctgac gccatccggt cagcgcgccg agctggcgat cccacagctc
    14821 acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt
    14881 accggacagc acggcgtgat gcaggtgcgt atggatttgc gcgatgatga ggggttgtta
    14941 agcaatggtc gcgtctggct ccaggcggat gacatcgacc tgaagccgtg gctcggtaaa
    15001 tggatgcagg acaatattgc gctggaaacg gcacagttct cccttgaagg ctggatgacg
    15061 atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg tgccagctgg
    15121 ttgggcgaga aggaaacgca tacgctgtcg gtggataatc tgactgcgca tattacgcgt
    15181 gaaaatccgg gatggcagtt ctctattccc gatacacgga tcacgatgga cggcaaaccc
    15241 tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg cggcaaagac
    15301 aataaacgca gtgacgaact ccggattcgc gccagtaatc tggagctggc aggcctggag
    15361 ggcgtacgcc cgctggtcgc gaaactttca cctgcactgg gtgatgtttg gcgctccaca
    15421 caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc ggcagacaag
    15481 acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt attaccgggt
    15541 gcggaacact tctccgggac gctttccggc agcgtagaaa atggtttgct tactgcgtcg
    15601 atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactgga aatcaccgac
    15661 ggccaggcaa cgataagctg gctgaacaat gacaaaggtt tccagctgga tgggcgtaat
    15721 attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct
    15781 aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg ttcacaagcc
    15841 tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattactt aagtggcgcg
    15901 attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc gcaactcttc
    15961 ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt
    16021 gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga ctttattaac
    16081 gacggtttat ggatgaaaac cgatggcgtt aatttgggcg gcgtgcgcgc gagtaatctc
    16141 accgcagtga tccctgacta ctcaaaagaa aaactgctga ttgacgctga cattaaaggt
    16201 ccgggtaaag ccgttggccc ttactttgat gagacaccgc tgaaagattc tctgggtgcg
    16261 accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct tgatatcccg
    16321 ctgaacggcg agttggtaac cgcgaaaggg gaagtgacgc tgcgtaataa cagtctgttt
    16381 atcaaaccac tcgacagcac cctgaaaaat ttgagcggta aattcagctt tatcaatggc
    16441 gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa cgtggatttt
    16501 tccaccaaag aaggggcaaa agcctaccag gtcgcggtga atctcaacgg taactggcaa
    16561 ccagcgaaaa ccggcgttct gcctgcagcg gtgaacgaag cattgagtgg cagcgtggcg
    16621 tgggatggta aagtgggcat tgatctgcct tatcatgctg gcgcgaccta taacgtagag
    16681 ctgaacggcg atttgaagaa tgtgagcagt cacttacctt caccgttagc caaacctgcg
    16741 ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt tgaattaacc
    16801 ggacaggctg gtgcggataa ccatttcaat agccgctggt tgctcggtca aaagctgacg
    16861 ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt
    16921 ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt ggctggccct gttccagaaa
    16981 ggcgcggcgg agagtgtcgg tggtgcagcg agtttcccac aacacataac gttacgtacg
    17041 cctatgttgt cgctgggaaa tcagcaatgg aataacctga gtattgtttc gcaaccgacg
    17101 gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca acgccacgct ggcgatgcgt
    17161 aataacgcac cgtggctggc gaatatcaaa tatctttatt acaacccaag cgtggcgaaa
    17221 actcgtggtg attcaacaca gtcatcacct ttcccgacaa cggaacgcat taacttccgt
    17281 ggctggccag acgcacaaat acgatgcgca gagtgctggt tctgggggca aaaattcggt
    17341 cgtattgaca gtgatatcac catttctggc gatacgttaa cgctgactaa tggactgatt
    17401 gatactggtt tttcgcggct cactgccgag ggtgaatggg ttaacaatcc ggggaatgaa
    17461 cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg atgccgccgc agaatttttt
    17521 ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt acactggcgc
    17581 aaagcaccct ggcagccaga tgaggcgacg ttgaatggca tcattcatac tcaactgggt
    17641 aaaggcgaaa ttaccgaaat caataccgga catgccgggc aattgctgcg cttattgagc
    17701 gtagatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg cgaagggttc
    17761 tattttgact ccattcgcag caccgcgtgg attaaagacg gcgttatgca caccgacgac
    17821 acgctggtgg atggcctgga agcggacatc gccatgaaag ggtcggtaaa tctggtacgt
    17881 cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt cggcgtggct
    17941 gcggcctttg cggttaaccc cattgttggc gcggcagtgt ttgccgccag taaagtgctg
    18001 ggtccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc gctggacgat
    18061 ccgcaaatca acgaagtgtt gcgacaaccg cgtaaagaaa aagcgcaatg atttgacgag
    18121 ggcgcgtaat tgccccaatc tcatatgata atcgttgcca aaggccaacg agccagaaca
    18181 taaccgtagg ccggacaggg cgttcacgcc gcatccggca gccgtaaaaa atcctctact
    18241 gcagtaacta acgagtagca aaaacgatga gtctgaacct ggtaagtgaa caattgctag
    18301 cggcgaacgg cctgaaacat caggacttgt tcgcgatcct cggtcaactg gccgaacgtc
    18361 gccttgacta tggcgatctc tattttcagt cgagctatca cgaatcctgg gttttagaag
    18421 accgtattat taaagatggt tcttacaaca tcgatcaggg cgttggcgtg cgtgcaatca
    18481 gtggtgaaaa aaccggattt gcttatgctg accaaatcag cctgctggcg ctggaacaga
    18541 gtgcacaagc ggcgcgcacc atcgtccgtg atagtggtga tggcaaagta cagacgctgg
    18601 gcgtggtaga gcatagcccg ttatatacct cggtagatcc gctgcaaagc atgagccgtg
    18661 aagagaagtt ggatattctg cgtcgcgtcg ataaggttgc ccgcgaagcg gacaagcgcg
    18721 tacaggaagt gactgccagc ctcagtggtg tctatgaatt aattctggtt gcggccaccg
    18781 atggcacact agcggcggat gtccgtccgc tggtgcgtct ttccgtgagc gttttggtcg
    18841 aagaagatgg caaacgcgaa cgcggtgcca gtggtggtgg cggtcgtttt ggttatgagt
    18901 tcttccttgc cgatctcgac ggcgaagtcc gtgcggatgc gtgggcaaaa gaagcagtgc
    18961 gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc gggcaccatg ccggtagtac
    19021 ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt gggtcacggt ctggaaggcg
    19081 acttcaaccg ccgtggcact tcagtattta gtggacaggt cggggagttg gtggcttcag
    19141 aactgtgtac cgtggttgat gatggcacga tggtcgatcg ccgtggttcg gtggcgattg
    19201 atgacgaagg tacgccaggc cagtacaacg tgctgattga gaatggcatc ctgaaaggct
    19261 acatgcagga taaactcaac gcgcgtttga tggggatgac gccgactggc aacggtcgcc
    19321 gtgaatccta cgctcatctg cccatgccgc gtatgaccaa cacctatatg ctgccgggta
    19381 aatcgacccc gcaggaaatt attgaatccg ttgagtacgg tatctatgca ccgaactttg
    19441 gtggcggcca ggtggatatc acctccggca aattcgtttt ctccacctca gaagcgtatc
    19501 ttattgaaaa cggtaaagta actaagccgg tgaaaggcgc aacgttgatt ggttccggta
    19561 tcgaaaccat gcagcagatt tcgatggttg gcaacgacct gaaactggat aacggcgtgg
    19621 gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt gggccagcca acgctgaaag
    19681 ttgataacct gacagttggc ggtaccgcgt aataatttca gtattttcag aaggataatt
    19741 aatccttctc cgtgtacgaa cggtcccctc gcccctctgg ggttagggtg aggggacccc
    19801 gttggcacag gtttgtacaa cgtaacagta caatattaat tacttctctt tcccgcgccc
    19861 gtgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgttagcg agttgatcac
    19921 gacttgtacc ttcagcggca gcttatcttt ttcggtatat aacgcataaa ccgggcgtgg
    19981 atctgactgg taacgtggta gcaggatctc cagttccccg cgattgatct cgttgatcac
    20041 ccacatcaac ggcacgtagg cgatcccggc acccgccgtc agccagcgga ccagcgtcat
    20101 cggatcatta gtcacaaatc tcccttgcgg gatcaggcga gtcgagatcc cttccggtgc
    20161 gatcagttca aattcattgt cgggtcgcac gctgtattca agccatgagt gactacttaa
    20221 atcggcgggt ttttccggta tgccgtattg tgtgagataa ctttttgcgg cgcacaccac
    20281 cattggcatc gcgcccagac ggcgggaaaa caggctggaa tcttgcaacg cgccgacgcg
    20341 gatcaccaca tccagaccgt cggcaatcag gtcgggggca ggaattccgg taaccagatt
    20401 gacactcaaa cctgggtatt ctttcagcat ttttgccgtc agcccggcga gaacattttg
    20461 tgccatagtt gaagaacagc caatgcggag cgtcccgatg ggggtgttgt tgaaggcata
    20521 cagttgctca tgaacatcct gcacttcatg gagcatccga cggcagccct ggtagtaaat
    20581 tctgccggct tcagtcaggc caatgctgcg tgtactacgg tttaacagct ttacctgtaa
    20641 ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct gtagctgtct
    20701 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
    20761 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
    20821 taacagttac gttaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
    20881 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctatacaa
    20941 ccaaggtcaa catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
    21001 tatttttcga attgctttta tcactggcga ttttctggct ggtgcgccgg gtacttgtgc
    21061 caacaggtat ttacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
    21121 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
    21181 aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
    21241 aatgcctggg tctattacac cgaatccccg tggacgcgtg acgcgcgctt tagcgctgac
    21301 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcatgataac
    21361 cagctggtga aaaaaggaca ggtactgttc accatcgacc agccgcgcta tcaaaaggcg
    21421 cttgaggaag cgcaagccga tgttgcttat tatcaggtac tggcacagga gaaacgccag
    21481 gaggccggac gtcgtaaccg tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag
    21541 gcgaacaacg tactacaaac agttctgcat cagttagcga aagcgcaggc gacccgcgat
    21601 ctggcaaaac tggatcttga acgtacagtc atccgcgcgc cagcagatgg ctgggtgacc
    21661 aacctcaacg tctataccgg tgagtttatt actcgaggat caacggcggt tgcgctggtg
    21721 aaacagaact ccttctatgt actggcctat atggaagaaa ctaagctgga aggggtgcgc
    21781 cctgggtatc gtgcggagat cacgccgctt ggcagtaaca aagtgctgaa agggactgtt
    21841 gatagtgttg ccgcaggggt caccaatgcc agcagcacgc gtgacgacaa agggatggcg
    21901 accatcgact ctaaccttga atgggtacgt ttggcgcaac gtgttccggt acgtattcgt
    21961 ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacggt ggtagtcact
    22021 ggcaaacaag atcgcgacga aagtcaggat tcgttcttcc gtaaaatggc tcatcgcctg
    22081 cgtgagtttg gttaatcacg atgggtattt tctccattgc taatcaacat attcgctttg
    22141 cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac ttccagctgg
    22201 aaacgccacg ctgggcggta ctgacagcgg cgattgttgc cgccggtccg gcctttgctg
    22261 cgggaggtga accgtattct ggcgctattc gctatcgtgg ctttttgcgc atcatcggca
    22321 catttattgg ctgtattgcc ggactggtaa tcatcattgc gatgatccga gcaccattat
    22381 tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc tcgctggtac
    22441 gaatagaaaa ctcgtatgcg tgggggctgg ccggttatac cgcgctgatc attgtgatca
    22501 ctattcagcc ggaaccattg cttacgccgc agtttgccgt cgaacgttgt agcgagatcg
    22561 ttatcggtat tgtgtgtgca attatggcgg atttgctctt ttctccgcga tcgatcaaac
    22621 aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta atgcaactct
    22681 gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgatctg gtgcgacgta
    22741 ctacggcgtt acaaggtatg cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg
    22801 ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc caatcctgcg
    22861 aaacttatct tattcagaat acgcgcccgg aattgatcac tgatactttt cgcgaatttt
    22921 ttgacacacc ggtagaaact gcgcaggacg tccacaagca gctcaaacgc ctgcggagag
    22981 ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg
    23041 caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg
    23101 aagaagagat cctgcaaggc gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg
    23161 caatggttaa cttctggcga accacacttt cttgcattct gggcacgctt ttctggctgt
    23221 ggacgggctg gacttccggc agtggtgcaa tggtgatgat tgcggtagtg acgtcactgg
    23281 caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctacggg acgctggcag
    23341 cgctaccgtt agggctgctc tactttttgg tgattatccc taatacccaa cagagcatgt
    23401 tgctgctgtg tattagcctg gcagtgctgg gattcttcct cggtatagaa gtacagaaac
    23461 ggagattggg ctcgatgggg gcgttggcca gcaccataaa cattatcgtg ctggataacc
    23521 cgatgacttt ccatttcagt cagtttctcg acagcgcatt agggcaaatc gtcggctgtg
    23581 tgctcgcgtt caccgttatt ttgctggtgc gggataaatc gcgcgacagg actggacgtg
    23641 tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat gtggcacgtc
    23701 gtaaagagaa tcacctcccg gcactttatc agcagctgtt tttgctgatg aataagttcc
    23761 caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcgcac cagcgcctgc
    23821 gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca
    23881 cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac tttggccagt
    23941 tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagcg ccaccgcagg
    24001 tgacggaacc agtacatcgg cttacgggta tgctccataa gtatcaacat gcgttgaccg
    24061 atagttaagt caaaaccgac gcctgaagcg tcggtttttt catggctata cttagcgatg
    24121 aacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta tacctttgat
    24181 tttgatgaga ttgagagtca ggaggatttt tatcgtgact ttagccaaac ctttggtctg
    24241 gcgaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa cgatgtcctg
    24301 ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg ccgttttggc
    24361 gcgttaatat tgctgtttga tgaggcagag gaagaactgg aagggcattt gcgttttaat
    24421 gttcgtcatt agcgcaaaaa aagcccccga accgggggca atatcgtcgg acaagacgat
    24481 gagggtttat ttgtacagtt cagccgtagc gtgccaggtg tcaccactac gagcttcagt
    24541 aatctggtag gccgttgcgc ctttctcttc cgcttttttg ttcagcattt cacgcatatc
    24601 cattggcgaa gacgccacac cacttacgga tacggtcccg attgcttcac gattttgtgc
    24661 ttgtgcagca tcaatggagt cggcagcgaa tgcaccgaaa gagagaacag aaagcacact
    24721 taatgcagca acagtggttt tgattttcat gatttttacc tcgacataat cttttagctg
    24781 ggcctttgtt tcgtgaccca tatcacaaaa tcaagtatac actaattatt gag
//
LOCUS       DADSZD010000061        24540 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.061, whole genome shotgun sequence.
ACCESSION   DADSZD010000061 DADSZD010000000
VERSION     DADSZD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.061"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            55..786
                     /gene="artJ"
                     /locus_tag="J1Z28_002168"
     CDS             55..786
                     /gene="artJ"
                     /locus_tag="J1Z28_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7717003.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(815..1942)
                     /gene="rlmC"
                     /locus_tag="J1Z28_002169"
     CDS             complement(815..1942)
                     /gene="rlmC"
                     /locus_tag="J1Z28_002169"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBA7717004.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKAADLKNLLADFPVEEW
                     CAPVSGLEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(1983..2471)
                     /locus_tag="J1Z28_002170"
     CDS             complement(1983..2471)
                     /locus_tag="J1Z28_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBA7717005.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(2531..3376)
                     /gene="potI"
                     /locus_tag="J1Z28_002171"
     CDS             complement(2531..3376)
                     /gene="potI"
                     /locus_tag="J1Z28_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBA7717006.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(3373..4326)
                     /gene="potH"
                     /locus_tag="J1Z28_002172"
     CDS             complement(3373..4326)
                     /gene="potH"
                     /locus_tag="J1Z28_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBA7717007.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            complement(4336..5469)
                     /gene="potG"
                     /locus_tag="J1Z28_002173"
     CDS             complement(4336..5469)
                     /gene="potG"
                     /locus_tag="J1Z28_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBA7717008.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDGDASVVDNVPVHVALRPEKIMLCDQQPADGYNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5564..6676)
                     /gene="potF"
                     /locus_tag="J1Z28_002174"
     CDS             complement(5564..6676)
                     /gene="potF"
                     /locus_tag="J1Z28_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBA7717009.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7026..7502)
                     /locus_tag="J1Z28_002175"
     CDS             complement(7026..7502)
                     /locus_tag="J1Z28_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBA7717010.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7590..8492)
                     /gene="rimK"
                     /locus_tag="J1Z28_002176"
     CDS             complement(7590..8492)
                     /gene="rimK"
                     /locus_tag="J1Z28_002176"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBA7717011.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8553..9275)
                     /gene="nfsA"
                     /locus_tag="J1Z28_002177"
     CDS             complement(8553..9275)
                     /gene="nfsA"
                     /locus_tag="J1Z28_002177"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBA7717012.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9259..9546)
                     /locus_tag="J1Z28_002178"
     CDS             complement(9259..9546)
                     /locus_tag="J1Z28_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBA7717013.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            9706..9963
                     /gene="grxA"
                     /locus_tag="J1Z28_002179"
     CDS             9706..9963
                     /gene="grxA"
                     /locus_tag="J1Z28_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBA7717014.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWAKENLDA"
     gene            complement(9993..10370)
                     /locus_tag="J1Z28_002180"
     CDS             complement(9993..10370)
                     /locus_tag="J1Z28_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717015.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            10640..12325
                     /locus_tag="J1Z28_002181"
     CDS             10640..12325
                     /locus_tag="J1Z28_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBA7717016.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(12501..13037)
                     /locus_tag="J1Z28_002182"
     CDS             complement(12501..13037)
                     /locus_tag="J1Z28_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBA7717017.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            13121..14329
                     /locus_tag="J1Z28_002183"
     CDS             13121..14329
                     /locus_tag="J1Z28_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7717018.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGL
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMMVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14329..15144
                     /gene="ybjI"
                     /locus_tag="J1Z28_002184"
     CDS             14329..15144
                     /gene="ybjI"
                     /locus_tag="J1Z28_002184"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBA7717019.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSTVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            15230..15514
                     /locus_tag="J1Z28_002185"
     CDS             15230..15514
                     /locus_tag="J1Z28_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717020.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(15555..16787)
                     /gene="mdfA"
                     /locus_tag="J1Z28_002186"
     CDS             complement(15555..16787)
                     /gene="mdfA"
                     /locus_tag="J1Z28_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBA7717021.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            17072..17668
                     /gene="ybjG"
                     /locus_tag="J1Z28_002187"
     CDS             17072..17668
                     /gene="ybjG"
                     /locus_tag="J1Z28_002187"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBA7717022.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            17726..18484
                     /gene="deoR"
                     /locus_tag="J1Z28_002188"
     CDS             17726..18484
                     /gene="deoR"
                     /locus_tag="J1Z28_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBA7717023.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(18531..19733)
                     /gene="dacC"
                     /locus_tag="J1Z28_002189"
     CDS             complement(18531..19733)
                     /gene="dacC"
                     /locus_tag="J1Z28_002189"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBA7717024.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLMEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            19980..20606
                     /locus_tag="J1Z28_002190"
     CDS             19980..20606
                     /locus_tag="J1Z28_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBA7717025.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(20603..21718)
                     /locus_tag="J1Z28_002191"
     CDS             complement(20603..21718)
                     /locus_tag="J1Z28_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HBA7717026.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(21829..22212)
                     /gene="bssR"
                     /locus_tag="J1Z28_002192"
     CDS             complement(21829..22212)
                     /gene="bssR"
                     /locus_tag="J1Z28_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBA7717027.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            22425..23750
                     /gene="rimO"
                     /locus_tag="J1Z28_002193"
     CDS             22425..23750
                     /gene="rimO"
                     /locus_tag="J1Z28_002193"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBA7717028.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(23797..>24540)
                     /locus_tag="J1Z28_002194"
     CDS             complement(23797..>24540)
                     /locus_tag="J1Z28_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7717029.1"
                     /translation="NAIFTVSEAMEHFSYLPTGLYVFAYKKDVYFQVCTLIIFFAALV
                     AVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDGGLFFSAADVKKLYSMYNSAFL
                     DDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNINDTFGHLLGDEVLMKVVKILK
                     SQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEIVHFQVGSINLSTSIGVAHSNE
                     CTTVERLKMLADERLYKSKKNGRAQISWQ"
BASE COUNT         6190 a         6415 c         5970 g         5965 t
ORIGIN      
        1 cccatgccgc agacacggcc aaaaatcata agattgacag acgggagttc catcatgaaa
       61 aagttagttc ttgccgcatt acttgcttct tttactttcg gtgcttctgc cgcagagaaa
      121 atcaattttg gcgtttcagc cacctatcca ccctttgaat ctataggtgc taataatgag
      181 attgtcggct ttgatatcga cctggcaaaa gccttgtgca aacaaatgca ggcagaatgt
      241 acttttacta atcacgcttt cgacagcctg atcccgtcac tgaaattcag aaaatatgac
      301 gccgtaatct ccggaatgga tatcacgccg gagcgtagca aacaggtatc gtttaccacg
      361 ccctactatg aaaactcagc cgtcgtgatt gccaaaaaag atacctacaa aacgtttgcc
      421 gatctgaaag gcaaacgtat tgggatggaa aacggcacca cgcaccagaa atatattcag
      481 gatcagcacc cggaagtgaa aactgtctcc tatgacagtt atcagaatgc ctttatcgat
      541 ctgaaaaatg gccgtattga tggcgtattt ggtgacaccg cagtggtaaa cgaatggctg
      601 aaaaccaatc cgcaactggg cgttgctact gaaaaagtga ccgatccgca atacttcggc
      661 accggcctgg gtatcgctgt acgtccggat aacaaagctc tgctggaaaa actgaataac
      721 gcgctggcag caattaaagc tgacggtaca tatcagaaaa tcaatgacca gtggttccca
      781 cagtaagatt cagaaagccg cacctgcggc ttttttattg cttcaccagt agcgtcagca
      841 cttcatagtg cgcggtgtgc gggaacatat caaaaagctg tacccgttcg atgcgataac
      901 caggcagttc gcggatatct ttcgccatag tttgggcgtt acagctggag tagataataa
      961 aacgcggtgc catcgttgag agataatcac acagcggttt accgatgccg cggcgcggcg
     1021 ggttaaccag caccagctcc ggcacttccc cctgagcggt ggcaaactgc gtagagtcca
     1081 gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc tgactgcttc gcacaggcaa
     1141 tggcctctgg tgcaatttcg atcccggtta actgcatgtc aggcgtcgcg cagtgtaaac
     1201 caaagccacc cacaccacag aacagatccc acatatgttt aaccggcagc tgtcgtaccc
     1261 agtcgcgcgc ggtggcgtac agctggctgg cgaccgccgg attagtctgg aagaaacttt
     1321 gcggacggat ccacagcggt acgtcattaa aacgctctgc cagtgcctgt tgttcggtca
     1381 ggtagatctc cgtctcccct tccataatcg ccatatgtac cggctgaata ttgacggtaa
     1441 taactttcag ctgcggtaat tgttcctgta accacggcag cgccttacgc agttgcgcca
     1501 gtttggtatc agaacgcagt acaaaacgca gcatcatgcc gccatcgctc tggctttcag
     1561 tcagcagaat gtatttcagt tcaccacgtt tacgcgccac gttgtagggc gttaaccccg
     1621 cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc aaatgaggct ggataaagcg
     1681 ggcagtcaca aaggtcttcc ggtgtaccat ctcgatgcag cataccgagc agtggttttt
     1741 caacgctacc actcaccacc attttggctt tattacgaaa cccttgttcc aggcctgaca
     1801 ccggcgcaca ccattcctca accggaaaat cggcgagcag atttttaaga tcggcggctt
     1861 tagcggagag ttgctctgga atcggctgcg ttatccactg acaggaacga cagcgacccg
     1921 cgtcgtaaag tgcgcactgc atacattgac cttcacatca tcaggggcga cgattataca
     1981 cattattgca actggaagaa ccgccgactg gtcgagggaa cgaacagcag catcaggatg
     2041 agcatatccg gcagcttctg cagcatcagg ctatggaaga tttcacgttt tgattcaccg
     2101 ggaatgctga acagctccgg ataaccatac cccagcgagg ccgcccacaa gtaacttgcg
     2161 gctgtgattt gtgtcaacag atacagccag cgcgcccagc gacgcccttt caccagtgaa
     2221 aacgcacacc agatctcaat gaaaaccagc accagactgc tcaaaaagac cagcgttaaa
     2281 ctccaggttt gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc cagcgtattg
     2341 aaaatcatca gcacgtcgag gccacggatc ataataatgg cgagcgccgc cacctgcacc
     2401 agcgcaggca cattcaggcg agcatgagat gaagatgttt tcttaaaaaa tcccaacgtt
     2461 tcgtcttcca tgaaaacaat gccgcgacat gcgcggcatt atgtagccag gttggcaaat
     2521 tttagtgtct tcagccacgt cttgcacgct ggatgtcgcg tatccgctgt ttttctgcgc
     2581 gcgccatcag ataccaggcg ataaatccga caattccgac cgcgccgagg atcaatgttg
     2641 ccagggcgtt gatttccgga ttcaccccca tccgcacgct ggaaaagacc agcatcggta
     2701 acgtggtggc tcccggcccg gaaacaaagc tggcgataac cagatcatca agcgacaaag
     2761 taaaagccag taaccagcca gaaatgatcg cgggcatgat catcggtagc gtaatgacga
     2821 aaaacacttt cagcggcgtc gcaccaagat ccatcgctgc ttcttctatc gagctatcca
     2881 gttcccgcag acgcgacgaa ataacgactg ccacataagc cgtacagaaa gtgacatgcg
     2941 ccagccagat ggtgagcata ccgcggtccg caggccagcc aatggcatga gcaagcgcga
     3001 cgaataacaa caatagcgac aagcccgtga tgacatctgg catcaccagc ggcgcggtga
     3061 tcataaaggc aaaaccattt gatccgcgaa acctgccaaa gcgcaccagc accaccgccg
     3121 caatagtccc gaggatcgcc gccgccgtcg ccgcacaggc cgcaattgtc agacttaaac
     3181 caaccgcact catcatcgcg tcatcgcgca ataattcgcc ataccagcgc gttgaccagc
     3241 cggcccacac cgtcactagc ttcgagctgt taaacgaata gataacaagc atcagcattg
     3301 gcgcgtagag aaaggtgaag cccaacagca aaatcacaat ccgccagggc gaacgaacca
     3361 ccggtaaatt attcatccgt gttctcccac gcttttttgc tgatgtttgt ggaaccacat
     3421 tatcggcata attagcagca gcaacatgat gatcgctacc gctgaggcca ccggccagtc
     3481 gcggttgtta aagaactcct gccacagcac gcgcccgatc atgatgctgt ccgggccacc
     3541 gagcagttcc gggatcacaa actcgcccac agccgggata aacaccagca tcgatccggc
     3601 aataatccca cctttagtca gcggcacgat cacagtaaag aacgttttca gcggtcgtgc
     3661 accgagatcc agtgccgctt ccaccagcga ataatcaata cgaatcaacg cggtgtaaat
     3721 cggcagcacc ataaacggca cgtaggcgta aacaatgcca atataaaccg caaagttggt
     3781 atgcagaatg gtcagcggtt gatcgataac ccccagccac agcagaaaat tattcagcac
     3841 accgttgttt tttaatattc ccatccaggc gtagacacga atcagaaacg aggtccacga
     3901 cggcaggatc accagtagta ataagatatt acgggtcgaa ggcttactgt gcgccaccgc
     3961 ccacgccagc ggatagccga tcagtaaaca gcaaatagtc gaaatcgccg ccacctgtaa
     4021 cgactggaga taagcatcga aatagagcgg atcgtcggtc agttgcagaa aattaccgag
     4081 attaagagtg atagaaagtt gcccgtcagc ccactccatc aattcggtat aaggtggaat
     4141 agcgcgcgcc atctccgcca ggcttatttt aaagacgatc agaaatggca gcagaaacag
     4201 caagatcaac cagatatatg gcaacgcaat gaccagtttg cgcccatgct tcatttgcag
     4261 ctgcgacaac cacagcttaa atccgcccgg cggtttcgac tgggcagcag gttcaagtgt
     4321 actcattttt gctccttaaa ccgtcagcac cacacagctg tccacttccc agcacaaacg
     4381 cacttcatcg ccccaggtcg gtaacccttt acgatgacga tgggcgtttt gtagctgggc
     4441 gctgatcatc tgcccacttt tcagacgaac gtgatacacc gaaagatcgc cgagataggc
     4501 aatgtgtatc acctccccca ccgcaaaatt gtagccgtca gcaggctgtt gatcgcaaag
     4561 catgattttt tccggacgca gcgccacatg caccggcacg ttatcgacca ccgaagcatc
     4621 tccatcgact ttcagtggat gcaccagccc cggcgaataa agcaccagac catcttcctg
     4681 acgctctttg agtacacctt caaagacatt aaccgagcca ataaattcgg cgctgtagcg
     4741 agtggtcgga tgctcgtaga tctcttcagg ttcgccaatc tggacaaatt tcccacgatt
     4801 cataatggcg atacgccccg ccatggtcat cgcctcttcc tgatcgtggg tgaccatcac
     4861 acaagtcaca ccgacacgct ccagaatatc caccacttca agctgcatcc ggtcacgcag
     4921 ctttttatcc agcgcgccca tcggctcatc gagcagtaat agtttcgggc gcttggcaag
     4981 gcttcgggcc agggccacac gctgacgctg accaccagaa agctgatgcg gtttgcgttt
     5041 ggcgaactcc tgcatatgca ccagcccaag catctcattg acccggctgg caatttccgc
     5101 tttcggtagt ttgtcctgtt tcaggccaaa agcgatgttc tgttccacgg tcatatgcgg
     5161 aaacagcgcg taagactgaa acatcatatt gatggggcgc aggtaaggcg gcacctgtga
     5221 caaatcgacg ccatcgagca taatctgccc ggcagaaggt tgttcaaaac ctgccagcat
     5281 acgcagcagc gtggacttgc cacagccgga tgcgcccagc agcgcgaaga tttcaccttt
     5341 gtagatggtc aggctgacat catccaccgc atgttgacca tcgtaggatt tggtcaggtt
     5401 acggatttct aaaagcggcg tcagcgcctt acgggttttc gcctgcgggc gagggatagc
     5461 gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat ggtgcgaatt
     5521 gccggcgggt gtggtgcgcc cgtccggcat ctacggctgc ggattatttt ccgctcttca
     5581 ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact ttcagagtga
     5641 acagcttcgc acgaacatcc gcaggcggat aaatacccgg gttatcacgg acttccgcac
     5701 tcaccagcgg cgtggctgct ttattggcgt tagcatagaa cacatggtca gagatatgtg
     5761 ccaccacatc aggacgcagc aggtaattca ggaactgata ggcttcgtct ttgtttttgg
     5821 catccgcagg catggcgaat acatcaaaga acgccatcgc cccttctttt ggaatcgaga
     5881 acgagacatt cacgccattc ttcgcttcct tcgcgcggtt tgacgcctgc cagacatcgc
     5941 ccgcccagcc aatagcgaca cagatgtcac cattcgccag atcattgatg tattgggatg
     6001 aatggaaata acgaatgttc gggcgcagct ttaacagcag atcggtagcc ggtccggtgt
     6061 aatcatccgc tttagtgctg ttaggatctt tgccgagata attcagcacg gtagcaaaca
     6121 cttcttctgg cgcatccagg aaagagacac cgcagctttt cagtttttcc agattttcag
     6181 gtttgaggat caagtcccag ctatcgacgg gcgcgttttc gcccaacacc gctttaactt
     6241 tatcgacgtt atagccaatc ccggtcgtcg cccacatata gggcatagca aatttattgt
     6301 cgggatcgtg tttggcgacc aacttcagca gctccggatc gagattcttc cactccggca
     6361 atttgctttt atccagcggc tggaaaactc ccgctgtcag ctggcgctca agaaagcttg
     6421 ctgacggcac caccagatca aagccagtgc tccccgccat taatttgcct tccagtactt
     6481 cgttagaatc gaaaacatcg tagacgactt taataccggt ttctttttca aagttggcca
     6541 ccgtgtccgg ggcgatataa tcagaccagt tataaatgtg gagtgttttt tgttcagccg
     6601 cgagcgtgct gacagagacg gccatcagag cccccgcaac cagacccgat agccattttt
     6661 tatttaaggc ggtcatatct ctttccttct gaaaattcgt taacaaacga acaaaagttg
     6721 tcacactgaa agatatgcaa taaacgtgca tatttttatc ggcgcggtgt aagaaaagag
     6781 aactctctcc cggcctgcac tgctcactat ataaaagcat cgcaagaata actttagcca
     6841 gggtttgaga ctatagctcg gattaaaaaa cggtatttaa caaattttta tgcgtatttg
     6901 ctctatgtga taaagccaaa tcggcacaag agaagtgaat taatctttaa taaaaggtta
     6961 aaaacagcgg ataattttgc ggcacgcagt cactgcggca gcgactgcgt gctcgaaagg
     7021 agttatcagt ggaggaaata gttttcggta acgttatttt gcccttcatc gtcagtcggt
     7081 aacagcagtt gatgggcatt agcttcaaga atgaccatcg aaatctgctc ttcgctctga
     7141 cgcacgaacc atgcaaactg ctcataggtt acgccctgca taacggataa agactggcaa
     7201 accaccagct ttggcagatt atcatcctgc atatcaagaa atgctttcac ggtcagcgaa
     7261 ctggcattga tggctgataa atccgccgcc agcggcaata cggctgaagg tcggacttcc
     7321 gccatggcag aaaacaggat cgtgttatcg atcagatcga ttttagcatc aaagacaccg
     7381 tcgaaattct gcatatgggg cagatgcaga gcctgacagt tatcacattc aaaaaaactc
     7441 atccccaggt catcgagcca ttgacgcaac gtatccagac caggaacgac cagcgatgtc
     7501 ataattttgt acaacctctt ccgataaaaa gaccggcaca gcttacgcaa aaagcgcagg
     7561 caaaaccatg atcagtaatg tgattgcgac taaccacccg ttttcaggca atattctgtc
     7621 gtagcgtggc gttcgatcca gcggatcatt ttacccgcga tgtcgatacc ggtggttttt
     7681 tctattcctt ccagccctgg cgacgcattc acttccatca ccaacggccc gcgattagca
     7741 cgcagaatat ccacaccagc aacgtccagc gccatcgttc gcgcggcttt tatcgcgatt
     7801 tcacgctcct gtggtgtgat actggcgacg cttgccgcgc cgccacgatg caaattggaa
     7861 cgaaaatcgc cctctttcgc tcgccgttca atcgcagcga ctacttcatc gccaacaacc
     7921 agacagcgga tatcgcaccc ttgcgcctct ttgatatatt cctgcaccag aatatgcgcg
     7981 ttcagaccgc ggaaagcgtc aatcacgctt tcggccgcct gacgcgtctc cgccagcacg
     8041 acaccaattc cctgcgtgcc ttcaaccagc ttgaccacca gcggcgcacc accgaccatg
     8101 tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc ccgtgacagg caggtcgatg
     8161 ccctgacgcg ccagcagttg catggaacgc aatttgtcac gcgcccgggc aatggcgacc
     8221 gactcattga gcggatagct ccccagcatc tcgaactggc gcagtgccgc cgtcccataa
     8281 aaggtaatgg cggtgccaat acgcgggatc actgcgtcaa aatggggtaa cttgcggcct
     8341 ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt agcaagaaag cggatcaaga
     8401 atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca gccgcttaca cgaatagagc
     8461 gttccatccc gggacaatat ggcaattttc actctgcacc tctctgtcag acctggtcaa
     8521 aagcccgcgt atcatacatc gacgtggcag ttttagcgcg tcgcccaacc ctgtttgtgc
     8581 aaataatcca gaataaatgg gcggctttct ttaatgattg ttcggcggat atgatcgctc
     8641 caggtatccc ggcgattatt gctgccacgg gtgaggtaat attccgccag ttgctcgtca
     8701 tactgcgcca gtgcgtcttt atccagcggt tgatagctgt tttcatgcac caaaatggag
     8761 gccggtaaac gcggcttaag atccggatta tccgcaggcc agccaaggca cagcccaaac
     8821 agcggcagaa catgctgcgg taatttcagc agttccgtca ccgcttcaat attattgcgc
     8881 aggccgccga tatatacccc gcccaatccc agcgattccg ctgcggttaa tgcattctgt
     8941 gccatcattg ccgtatcaac gacaccgagc aacagttgtt ccgccaggcc gagctgagca
     9001 tccggacaga tctgtaaatg gcggttaaag tcagcacaga acacccaaaa ctccgccgct
     9061 tgcgctacgt gtttttgccc gccggtcagc gtcaccagtt cttcacgtaa cgctttgtcg
     9121 gtaatgcgaa taatgctact gcactgcaaa aaactggaac tggacgtcgc acgggcgctg
     9181 ttaataatcg cctcacgctg cgcttcggaa atgggttcat cagtgaaatg gcgaatggag
     9241 cgatggccac aaataagttc aatggttggc gtcattatct ttttctcttt ctgaacgtga
     9301 atattgcggt ggatggttca tcagctgtgg ggcaagacgt tttgccacct gaagaataac
     9361 caccaccgca gcgggaagca tgagcaaaac accgagaaaa atcatcagaa tctgcacttc
     9421 tggccgagaa aatggctcag gtagcgacag ggagtcgctt accgacagca gcgccaccgc
     9481 cagcaacatc attccgataa attccagtat caacacgctt ttaggcaatt taccgatcgc
     9541 gcgcatacgc ttccctctgc aaagtgagcc ttcagtctaa aacttttcac tgtattgtgt
     9601 ttaacagtta tagcttttat caattaatgc aacaggttaa acctactttc agcgaataca
     9661 ttttagcgtg atcattacag gcataaatct atgaggagag aaataatgca aaccgttatt
     9721 tttggtcgtc cgggttgccc ttactgtgtg cgtgcaaaag atctggctga gaaattgagc
     9781 aatgaacgcg atgatttcca gtatcagtat gtagatattc gtgcggaagg gatcactaaa
     9841 gaagatctac aacaaaaggc aggtaaaccc gtagaaaccg tgccgcagat ttttgtcgat
     9901 cagcaacata tcggcggcta taccgatttt gctgcatggg cgaaagagaa tctggacgcc
     9961 tgatcgtctg acaagccctc gcgttgaggg ctttattgat tttttctgtg ctgtggttta
    10021 aacaaactac tgataaataa gaaacacaat gcccccagcg cacaccagaa caccgcactt
    10081 aataaccatg ccagctcttg ccagaatgag cgcgtcggtg aaaaaaacag ccgcataatg
    10141 agcatcgaac agggtgccgc cagcattgcg ccaaacagag gtttcaggac ttctctacga
    10201 tgtgaaaaga agctggcaac tgcaccagga agaatgaaaa atagcaaacc gatttcagga
    10261 tgcccggcag cccgaaaagc gcctttcatg tgcgtcgcca gaaaaagaca caccacaatg
    10321 aagaggacaa aacagcagat tgcccccgcc caacgttgtt tatgtttcac tcgttcctcc
    10381 tgacactgcg tctatcgaac acatttttcg ccagtgtggc gttcagtaag ataaagccgc
    10441 ttcgcattcc atgctaatat aggccaacgc aattcatata gccgttgata cctaatgtga
    10501 ttacactagt aaaatatatt gttactttac tatcgtttag gtgcgctgaa tgaatctgcg
    10561 ccctgaattc tggtaaaaaa cattatcgta aattaccatt tctttcaaca gcttactagt
    10621 aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa ttgttaaatg ggaattacat
    10681 tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc gggaaattac gacttggttc
    10741 gatccaactg ggtaattcca ttggcgtttt agtcgtatcg ctgttattag gccaacaaca
    10801 tttcagcatt aacaccgacg cgcttaatct tggctttatg ctgtttattt tctgcgtcgg
    10861 ggtcgaagcc ggaccgaact ttttttccat tttttttcgc gatgggaaaa attacctaat
    10921 gttagcactg gtgatggttg gcagtgcgct ggtgatcgcc ttagggttag gtaagctgtt
    10981 tggctgggat attggcctga cggccggtat gttagctggc tctatgacgt cgacaccggt
    11041 tctggtcggt gctggcgata cactgcgtca ttccggcatg gaaagcaggc agctctcact
    11101 ggcactggat aatctgagcc tcgggtatgc cttaacctat ttaatcggtc tggtgagttt
    11161 gattgttggt gcgcgttact tgccgaaatt gcagcatcag gacttacaga ccagcgccca
    11221 gcaaatcgcc cgcgaacgtg gcctggacac tgatgccaac cgtaaggttt atttaccggt
    11281 gatccgcgcc taccgcgtcg gcccggagct ggtggcctgg accgacggca aaaatctgcg
    11341 tgaactgggt atttatcgac aaactggctg ctacattgaa cgtattcgac gtaacgggat
    11401 tctggcgaat ccagacggtg atgctgtgct acaaatggga gatgaaatag cgttggtagg
    11461 ctatcccgac gcccacgcac gactcgatcc cagcttccgt aacggcaaag aagttttcga
    11521 tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg gtcgttaaaa accataacgc
    11581 cgtgggcaaa cgtctcgcac aactgaagtt gaccgatcac ggttgcttcc ttaaccgcgt
    11641 cattcgtagc cagattgaga tgccgataga tgacaacgtc gtgcttaaca aaggtgacgt
    11701 tttacaagtc agcggcgatg cccgtcgcgt aaaaaccatc gccgatcgca tcggctttat
    11761 ctcgattcac agccaggtca ctgacttgct ggcattctgc gccttctttg ttattgggct
    11821 gatgatcggg atgatcacct tccagttcag cacattcagt ttcggcatgg ggaacgctgc
    11881 cgggttgtta ttcgccggaa ttatgctggg ctttatgcgc gctaaccacc cgaccttcgg
    11941 ttacattccg cagggtgcat taagcatggt gaaagagttc ggcttaatgg tgtttatggc
    12001 aggcgttggt ctgagcgccg gtagcggtat taataacggc ctgggcgcga ttggcggtca
    12061 gatgttgatt gccggattga ttgtcagtct tgtgccagtg gttatctgtt tcttgttcgg
    12121 tgcttatgta ttgcgaatga accgcgcact gttgttcggc gcaatgatgg gcgcacgcac
    12181 ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc agtaacatcc cggcgctggg
    12241 ctatgcgggc acctatgcaa tcgccaacgt cctgctgacg ctggcaggga caatcatcgt
    12301 catggtatgg ccaggattag gataaaactg aagttgccct gaaaatgaaa ttttttttgc
    12361 acaaccgcag aacttttccg cagggcatca gtcttaatta gtgccactgc ttttctttga
    12421 tgtccccatt ttgtggagcc catcaacccc gccatttcgg ttcaaggttg atgggttttt
    12481 tgttgcctga aatttatcta ctacccagca atcctctcaa ccatcctcaa aatctcctcg
    12541 cgtgatagcg gcttacggtc ggtgacaaaa tgcagcgtca tcccctcaat aaacgcatca
    12601 agcgcgcggg cggttccggg ttcaaaccat tgttcgagcg tttgttgact gcgctgcatc
    12661 cagttttgca ttaccgtttt taagagcggt tttcggctag ccagcgcgta gagctggtac
    12721 atcagctcca tattatccgg cgttgcgacc tgtgagctgt agatcatatc ggtgatagcc
    12781 tggcatgcgc ctggagcatc actaacatcg ctaaaaaatg cctgatattg ccgggacatg
    12841 atctcagtaa aacggctgaa cgcctccagc aacaactcat caattcctga aaaatagtag
    12901 gtcatcgatc ccaacggcac cccggcaagg gtagcaattt tgcggtgtgt aacagcatgt
    12961 attccgtaaa gtttcaccgc ctccagcgtg gcctggataa ttttttctcg ccgttgcgga
    13021 tcgttagcgc gacgcatgtc atttcctctc tctgcaattg tgtacaaatg tacacagtct
    13081 tgctaatgtt gtcttccctc ttcttatttt gacgtggtct atgaccgtaa attcttcacg
    13141 taatgcattg aaacgccgaa cctgggcgct gtttatgttc ttctttttgc caggcctgtt
    13201 aatggcgtcc tgggcaaccc gtacgcctgc tatccgcgat attctctctg tctcgatcgc
    13261 tgaaatgggc ggtgttctct ttggtctgtc gatcggttcg atgagcggta ttctctgctc
    13321 ggcgtggtta gtgaaacgct ttgggacgcg taatgtcatc cttgtgacga tgtcctgcgc
    13381 attgattggg atgatgatat taagtctggc actctggcta acatcgcccc tgctctttgc
    13441 cgttggtctc ggcgtctttg gggcaagttt tggttctgcg gaagtggcga taaacgttga
    13501 aggtgctgcc gttgagcgag aaatgaataa gacggttttg ccgatgatgc acggttttta
    13561 tagcctgggc acgctggcag gcgctggtgt cgggatggca ctgacggcct ttggcgttcc
    13621 ggcaacggtg cacattttat tggcggcgct ggtaggcatc gcgcctattt atatcgccat
    13681 tcaggcaatc cctgacggta cgggcaaaaa tgctgccgat ggcacccagc atggcgaaaa
    13741 aggcgttcct ttttatcgcg atatccagtt gctgttgatt ggtgttgtag tgctggcgat
    13801 ggcctttgcc gaaggttctg ccaacgactg gttaccctta ttaatggttg atggtcacgg
    13861 ttttagccct acttccggct cgctgattta tgccggtttt accctgggga tgaccgttgg
    13921 acgctttacc ggcggttggt tcattgaccg ttacagtcgc gttgccgtgg ttcgggccag
    13981 tgcactaatg ggggcgttgg gtattgggct gattattttt gtcgatagcg cctgggttgc
    14041 tggggtgtct gttgtacttt ggggactggg tgcctcgctg ggcttcccgc tgaccatttc
    14101 tgccgccagc gataccggcc tcgatgcacc aacccgcgtc agcgtggtag ctacgaccgg
    14161 ttatctggct ttcctcgtcg ggccgccgct gctgggctat ctcggcgaac attatggatt
    14221 acgtagtgca atgatggttg tactggcgct ggttattctc gcggctattg tcgcgaaagc
    14281 cgtcgccaaa cccgatacca aaacgcagac ggcgatggag aatagttgat gagcattaaa
    14341 ttaattgcgg tagacatgga tggtactttc ttaagcgatc aaaaaaccta taaccgtgag
    14401 cggtttatgg ctcagtatca gcaaatgaaa gcacaaggca ttcgctttgt ggtcgccagc
    14461 gggaatcaat attatcagtt gatctctttc ttccctgaaa ttgctaatga aatagccttt
    14521 gtggctgaaa acggcggctg ggtagtgagc gaaggcaaag atgtttttaa tggcgagctg
    14581 tcgaaggatg cgtttgctac tgtcgtggaa catttgctga cgcgcccgga agtggaaatt
    14641 attgcctgcg gaaaaaatag cgcctatacc ctcaaaaaat atgacgatgc catgaaaacg
    14701 gtggcggaaa tgtattatca ccgtctggaa tacgtcgata actttgacaa cttagaagat
    14761 atcttcttta agtttggtct gaatctttcc gatgaactga ttccacaagt acaaaaagca
    14821 ttacatgagg ccatcggcga tattatggtg ccggtccaca ccggcaacgg cagcatcgat
    14881 ctgattatcc ccggcgtaca taaagccaat ggccttcgcc aactgcagaa attatgggga
    14941 atagacgaca gcgaagtggt ggtctttggc gatggtggta acgatattga gatgctgcgt
    15001 caggcaggct ttagttttgc aatggaaaat gccggcagca cggtcgtcgc agcggcaaaa
    15061 taccgggcag gctccaataa ccgtgaaggc gtactggatg tgatcgataa agtgcttaaa
    15121 cacgaagcgc catttaacca ataaaaaagg cagcacgccc ggcaacacaa tgcctgaatg
    15181 aaggctgcat aattcgcgga aattaacccg ctgcattatt tcaggcgaaa tgattatgaa
    15241 aaattgtcta ctgttgggcg cgcttttaat gggctttact ggtgtggcga tggcgcaaag
    15301 tgtcaccgtc gatgtgccga gtggatacaa agtggttgtg gttcctgatt cagtcagcgt
    15361 tccacaagcg gtcagcgtcg cgactgtgcc gcaaaccgtc tatgtggctc ctgcgccagc
    15421 tccggcttac cgcccgcatc cttatgttcg ccatcttgcc agcgtaggtg aagggatggt
    15481 tatcgaacac cagattgacg accatcacca ttaattcgag aatgcctgat cgcacaaagc
    15541 aatcaggcat ttttttaccc ttcgtgagaa tttcccatct gtttatcttt taaaaagata
    15601 accatcagcg acagccacaa aatgccgttg acaaggttga agagattaaa cagtccgttg
    15661 cccccgttca gccaggcatg tttgctgatt tcaataccga cggtaaagat cagcatttgc
    15721 agcatcccca tcgccgcaga aaccgtacct ttactcatat cgctggcaaa cagggttaat
    15781 cgcaccagtc ccgcattcgc cagaccaata ccgaaagcat aaatacttaa cccggcggtc
    15841 atccataaat acgcgtgcga tgagataacc gttgccgcag cagcgaccaa cagaccaatc
    15901 ataatcggcc agccacccat aataatcagc gaacgtacgg tgcggcgcga ggtcagacgc
    15961 gctaacagca agttacccgc aattaacgcc ccgaaaatag gcacttgcag caagccatat
    16021 tcatagctgc tcaactgctc accggtaata atgataatcg gcgactgggc gatccacgcc
    16081 aacaacggca ggctaacgaa tcccagcgcc agcgcccccg ccacaaagcg gccgttcttc
    16141 agcaccagct tatagtcacg accgagttct ttcagcgaca gtttctcgcc tatacgcgtg
    16201 gcggtttcag gcatagctcg ttgcagaccg aaaaaggaga tcgctgccaa tgcggcaaac
    16261 aatacaaaca tcccttccca gggcagcaca tggatccacg ccgcgcccac cagcggacca
    16321 agtagtggag caatcagcgc cacgttcgcc attagcgcgg tgatcttgat acaaacggcc
    16381 tcttcgaagg attcctgaat tgcggcgtat cccacagcgc caatgaaaca gaggcttatg
    16441 ccctgcaaga agcgcaacag ggtgaattgt tcaatgtttt gcgccagtaa tattgccaga
    16501 caggtgacga taaaccacac cactcccgcc aacatcaccg gacggcggcc aatacgatcc
    16561 gacaacggcc ccaggagcca ttgtaaaaac atcccgcccg ccagatacgc agtcatcgaa
    16621 gtaggaaccc aatcaatgcc cgcctgatat tgttccacca cggccaacat accgggttga
    16681 atcatatcgt tgccgatata ggttgaaaat tcgtaaagca ccagacagag agggaaaagt
    16741 aacgcctgac gtccaagcct ggcaccggaa gctaatttat tttgcatgca atttcttcgc
    16801 caataataat cgcgcaaagt ttaataaaag cgcagctaac gagaaagcga attttgtagc
    16861 tgaaaccacg gttaagcaca ttcttacatt attgcgagta tagctacgct ttctttaagt
    16921 tttatttaac ctttgcccgt tacaatcacc taccgtaaac aggccgcttg agggaaataa
    16981 gacgatgccg ctttacccag tttaacctgc actttattct caacgacttg cctgtattgg
    17041 ctccctttta atcactttgc gtcgggaagt tatgctggaa aatttgaatc tctctctatt
    17101 ctctcttatt aacgcaacgc cagactctgc gccgtggatg atctcgttgg cgatttttat
    17161 tgctaaagat ttgattaccg tggtgccgtt gctggccgca gtactttggt tgtgggggct
    17221 tacagcacaa cggcaactgg tgataaaaat cgctatcgcg ctggcggtca gcctgtttgt
    17281 gtcctggacg atgggacatc tttttccgca cgaccgaccc tttgtcgaaa atatcggcta
    17341 taacttcctg catcatgcgg cggatgactc attcccgagc gatcacggta cggtgatttt
    17401 cacctttgca ctggcatttt tatgctggca tcgcctgtgg tccggctcac ttttaatggt
    17461 gctggccgtc gtcattgcct ggtcgcgcgt ttatcttggc gtccactggc cgctggatat
    17521 gctcggtgga ttgctggcag gtatgattgg ctgccttagc gcccagatta tctggcaagc
    17581 gatggggcat aaactctatc aacgtctgca atcgtggtat cgcttctgtt ttgcattacc
    17641 gatccgcaaa ggctgggtgc gtgactgatc tctgctaaaa agtgtagtat tgagcggctc
    17701 gcttcaataa ctattcagag ggattatgga aacacgtcgc gaagagcgta tcgggcagct
    17761 gctgcaagaa ttaaaacgca gtgataagtt acatcttaaa gacgccgccg ccctgcttgg
    17821 ggtttcggag atgacgattc gtcgcgatct gaacaaccac agtgcgcccg tcgttttgct
    17881 cggcggctat attgttctgg aaccacgcag cgccagccat tacctgttaa gcgatcaaaa
    17941 atcccgcctg gtggaagaaa aacgccgggc ggcaaaactg gctgcgacgc tggtagaacc
    18001 cgatcagacc ctcttttttg actgtggcac caccacgccg tggattattg aagcaattga
    18061 taatgaaatc ccttttaccg ccgtttgtta ttcgctaaat acctttctgg cgctgaaaga
    18121 gaaaccccat tgccgcgcgt ttctttgcgg tggtgaattt cacgccagca acgccatttt
    18181 caaacccatc gattttcagc aaacgctgaa taatttttgc ccggatatcg ctttttattc
    18241 cgctgcgggc gtgcatgtca gtaaaggcgc tacctgcttt aatcttgaag agttgccggt
    18301 aaaacactgg gccatgtcga tggcgcaaaa gcatgtgctg gttgtcgacc acagtaaatt
    18361 tggcaaggtg cgtccggcgc gcatgggtga cctgaaacgc tttgatattg ttgtgagcga
    18421 ttgttgcccg gaagatgagt atgtgaagta cgcgcagacg cagcgtatta agttgatgta
    18481 ttaatgacgt ataaccggat gacgtttcgc gccatccggt tatcagaaga ttaagagaac
    18541 cagctgccga accactgatg gaatttcatc atcacgaaat cccacatccg accaaagaat
    18601 ccgccctctt ccacattttc catcacgatc agcggacgct gctcaatgga tttaccgtta
    18661 agctggaaat caatggtgcc gacaacctga ccttttttca gcggtgcggt aagctgcggt
    18721 tccattaacg tataactcgc cttcaggttt ttcagctgcc cacgcggtat ggtcactgag
    18781 cccgcttcgc ctgccccgag attcacttcg ctcttatcac caaaccagac gcgctgagtc
    18841 acaaatgtgg catcaggttt aattggcgtc acggtttcaa agaagcggaa accccaggtc
    18901 aataatttct cagactcatt aaaacggata cggtcagttt tcgcccccag cactacggag
    18961 attaaacgca tatcgccctg ggtagccgaa gcaaccagat tatatcccgc gcctgccgtg
    19021 gttcctgtct tcatgccatc aacattcaga ttgctgctcc acagcagacg attacggtta
    19081 ggctgacgaa ttttgttgaa ggtgaactct ttctctttat gaatggcgta ctcttccggc
    19141 acatcgtgga tcaatgcttt acccagcaat gccatatcgc gcgcggtact gaactgcccc
    19201 ggcgcatcca ggccgtgcac cgtctggaag gtagtgttgg tcagacccag ttttttggca
    19261 taaccattca tcaaaccaat aaatgactcc tggctcccgg caacgtaatc agccagcgca
    19321 atacaggcgt cattaccgga ctggataatc acacctttgt tcaagtctgc caccgaaacc
    19381 tgatcgcccg gtttgaggaa cattaccgat gaaccacgca gtgccggatt tcccgtcgcc
    19441 caggcatctt taccgaccgt caccatatcg gtgagtttaa tcttatcggc cttaagcgcc
    19501 tgcccaacca catagctggt catgatttta gtcaggctcg cgggatccag tttctcatcc
    19561 gcattgcctt ctgccagcac tttaccgctg gcgtaatcca ttaaaatcca tgcacgcgca
    19621 tccacgctcg gcgcttcaac ggtttgttcc gccgcgaatg ccgttggggc aaaaaggaat
    19681 aaaaatgcag aacccgctgc aagaccacga aggagagagg agtattgcgt cataaatgcc
    19741 acccgagtat ccattcaaaa aaaatacgtt atatcgccgt atgataagtt cggtgattaa
    19801 taacgcacac acaaacttaa agaaaccgta agttggtaaa gtttttaaag tttatgcaat
    19861 atcctgccgc ttcgctgctt ctggtgtcat ttttttgcca gctgttgctt aaaagttaac
    19921 tttacttcac catcggagtc ctgttcagcc tgtggctgcc tgtcggaaaa ggggtgagta
    19981 tgattacgct gtggggtcgg aataattcaa ccaacgtaaa aaaagtattg ctgacgctcg
    20041 aagaactgga actaccttat gagcaaattc tcgcgggccg tgagtttggg ataaatcacg
    20101 atgctgattt tctggcgatg aaccctaacg ggctggtgcc gttgttgcgt gacgacgaaa
    20161 gtgatctcat tctttgggaa tcaaacgcca ttgtccgcta tctggcggca cagtacgggc
    20221 aaaaacgcct gtggatcgac tcaccggcac gtcgtgcgga agcagaaaaa tggatggact
    20281 gggcaaacca gacgctcagt aatgctcatc gcgggatcct gatgggatta gtcagaacac
    20341 caccggaaga gcgcgatcag gccgccattg atgccagttg caaagagtgc gacgccctgt
    20401 ttgccctgct cgatgcggaa ctggcaaaag taaaatggtt ctcaggcgac gagtttggtg
    20461 tgggcgatat cgctatcgca ccgttcatct acaatttgtt taacgttggc ctgacctgga
    20521 caccgcgtcc aaatctgcaa cgctggtatc agcaactcac tgaacgcccc gcagtgcgca
    20581 aagtggtgat gattcccgtt agctaattgc gtgggctaac tttaagtaat tccccactgg
    20641 actcgtcggt gagaacgtat aaataaccgt cgggtccagt gcgaacatca cgaattcgct
    20701 gccctctgtc cgttaaaata cggccatctt ctgtcacttt gtcgccgttg acgctcatca
    20761 caatgacatc tttatctttc agcgcgccaa taaataattt ttgctgccac tgggggaatt
    20821 tgtcgctgtt atagaaggcc atgccgctca cggcgggcga atctttccag taaaaaacag
    20881 gttgctcggt cccggcgacg atctcccctt tcgcctccgg tatcttaaag cctgaatagt
    20941 tgattcccca ggttgccagc ggccagccgt agtttttgcc tttttgcggg atattaattt
    21001 catcgccacc gcgcgggcca tgttcattca gccatagtgc attactccac ggattcatcg
    21061 ccattccttg cggattacga atgccataag accagatctc ggcgcgcaca ccggattcct
    21121 ttataaaagg attatcatcc gggatttcgc ccaggtcggt cagacgcacc agtttgccct
    21181 gtaatttatc cagatcctgc gccgtcgggc gctgattgtt ttcgcccaga gcaataaaaa
    21241 gataaccttt accgtcgaat accagccgcc cgccaaaatg gttgccggta gacagttttg
    21301 gcatctggcg aaaaacggtg cggaagtcgg tcacttttga gagatcatca cttaagcggc
    21361 cataacccac agcagttccg gctttgccat catcgccaac ttcggaataa cttaaccaga
    21421 tgcggcgcga ctgagcaaaa tcgggcgcta aaaccacgtc cagcaggccg ccctgcccgt
    21481 gcgcccaaac gtccggcact ccagaaagcg gcgcagataa tccttttcct gcttgccagt
    21541 gacgcaactc gccgccgcgc agagtgatta acataccgtg attatcgggt aaaaaggcca
    21601 gcgcccaggg atggtcgagt ttgtcttgca gtacttcgac atttaccgtt gcaggagctg
    21661 cccagagagc ggaagaaaga caaataaggg gcacaaggaa aaaggattgt cgatgcataa
    21721 cgcctccgga ttgactctgg cttaaagcgt agtcagtgga ggagataaca aattcatttt
    21781 tacaaaaact taaacatgaa gggggagacg ctttctcccc cttagttttc aggccttctc
    21841 aagcatggcg tgcttctgca ggctctggat actcagcgtt aagctcatca gacaattttc
    21901 aagcttatcg gcgttgacgg taataacagt cgggcaatca tggtgcccac tcatcaaaca
    21961 tactgcggct gtcgctaatg cttcttcagc atgatgaaga gcactccact cttcctgatc
    22021 cagatgaaga ttcaaccgca gcgatttatc gtgcagttcg cgattcagtt taaaaaagtt
    22081 atctcgtaga tgattgcttt cgctgacgga catgtatcct tttgccttcc tcagttgcac
    22141 gtatgcggcg aggtcaacgc gtgcgtcgat caaccggttc agcagatcga ttcgctgtct
    22201 gtcaacgaac atacttcgat cctcctcttt ccagccttgt tgcaactaaa tagtatggct
    22261 gttttttgag caaaatgctg tccaggatca attcttcacg ccgaattaac aaaatttaca
    22321 tttccttcaa ctccgccagc gaatgagatt gcgatacaaa ttttatccag aactctgtag
    22381 aatccctgcc ctgacctttc ctgtaactga atatttttta agctatgagc aaagtaactc
    22441 cccagccgaa aatcggcttt gtttcccttg gctgtccgaa aaaccttgtc gattcagagc
    22501 gtattctcac cgaactacgc actgaaggtt atgacgtggt accgagctat gacgatgcgg
    22561 acatggtgat cgtcaacacc tgcggcttta ttgacagcgc ggtacaagaa tcactggaag
    22621 ccattggtga agcgttgaat gaaaacggca aggtaattgt gaccggttgt ctgggggcaa
    22681 aagaagatca gatccgcgaa gtccacccga aagtgctgga aatcaccggg cctcatagct
    22741 atgagcaggt tctggagcac gttcatcact acgtgccaaa accgaaacac aacccattcc
    22801 tgagcctggt gccagaacaa ggtgtgaaac tgacgccgcg tcattatgcc tatctgaaaa
    22861 tttctgaagg ctgtaatcac cgctgcacct tctgcattat tccgtctatg cgcggcgatc
    22921 tggtgagccg tccgattggc gaagtgttaa gtgaagcgaa acgtctggtt gatgcgggcg
    22981 ttaaagaaat tctggtgatc tcgcaggata cttccgcgta tggcgtcgat gttaaacatc
    23041 gtactggctt ccacaacggc gagccggtaa aaaccagcat ggtcagcctg tgcgaacagt
    23101 tatcgaaact ggggatctgg acacgtctgc actacgttta cccttatccg catgtggacg
    23161 acgtcatccc actgatggca gagggcaaaa tcctgccgta tctggacatt ccgttgcagc
    23221 acgccagccc gcgcattctc aaactgatga agcgtccggg ttctgtagat cgccaactgg
    23281 cgcgcatcaa acagtggcgc gaaatctgcc cggaactgac cctacgctca acctttattg
    23341 tcggcttccc tggcgagaca gaagaagatt tccagatgct actcgacttc ctgaaagaag
    23401 cacgtctgga tcgcgttggc tgctttaaat acagcccggt tgaaggtgca gacgccaatg
    23461 ccctgcctga ccaggttccg gaagaagtga aagaagaacg ctggaaccgt ttcatgcagt
    23521 tgcagcagca aatttccgcc gagcgcctgc aagagaaagt gggccgtgaa attctggtga
    23581 ttatcgacga agtggacgaa gaaggcgcga ttggtcgcag catggcagat gcaccggaaa
    23641 tcgacggcgc ggtttatctc aatggtgaaa ccaacgttaa gccgggtgat atcctgcgag
    23701 tgaaagtcga gcacgccgat gagtacgatt tgtggggtag ccgggtttaa ttaattcagg
    23761 ccgggcaaaa tcatttgtcc ggcctgcgaa taatgattac tgccagctaa tctgcgccct
    23821 gccgtttttc ttactcttat acagccgctc atccgccagc attttcaaac gttcaacggt
    23881 agtacattca tttgaatgcg ctacaccgat actggtgctt aaattaatgc ttcccacctg
    23941 gaaatgaacg atttctttta gaatcgatag caactcttcc agcgtgccgc cagtataaat
    24001 caccgcaaat tcgtcacctc cgaagcggta aactttacct ttatctaccg ggatctgtga
    24061 tttaaggatt ttcaccactt tcatcaacac ttcatcgccc agcaaatgac cgaaggtgtc
    24121 gttaatgttt ttgaatttat cgacgtcaaa caggcacaaa taaccgcctt tttccggcag
    24181 cgcttttaaa tcttcgtcaa aagattttct gcccatcgct ttggtcagat cgtccaggaa
    24241 tgccgagtta tacatactgt agagtttttt gacatcggca gccgaaaaga aaagcccgcc
    24301 atccagtacg cgttcaaaat ggttattaat aatggcttct ttctccacaa taccacgatt
    24361 aatcactctg cgtaccagat agaggcaact ggcacccgat atcactgcca ccaatgctgc
    24421 aaagaaaata atcaatgtac atacctgaaa ataaacgtct tttttataag caaatacgta
    24481 caatccggtc ggcaaatagg agaagtgctc cattgcttcc gaaacggtga agatggcgtt
//
LOCUS       DADSZD010000062        24429 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.062, whole genome shotgun sequence.
ACCESSION   DADSZD010000062 DADSZD010000000
VERSION     DADSZD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.062"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            635..2563
                     /gene="thrS"
                     /locus_tag="J1Z28_002195"
     CDS             635..2563
                     /gene="thrS"
                     /locus_tag="J1Z28_002195"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010245687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBA7717030.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2567..3109
                     /gene="infC"
                     /locus_tag="J1Z28_002196"
     CDS             2567..3109
                     /gene="infC"
                     /locus_tag="J1Z28_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBA7717031.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3206..3403
                     /gene="rpmI"
                     /locus_tag="J1Z28_002197"
     CDS             3206..3403
                     /gene="rpmI"
                     /locus_tag="J1Z28_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBA7717032.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3456..3812
                     /gene="rplT"
                     /locus_tag="J1Z28_002198"
     CDS             3456..3812
                     /gene="rplT"
                     /locus_tag="J1Z28_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016169860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBA7717033.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            3935..3979
                     /locus_tag="J1Z28_002199"
     CDS             3935..3979
                     /locus_tag="J1Z28_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl--tRNA ligase operon leader peptide"
                     /protein_id="HBA7717034.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4263..5246
                     /gene="pheS"
                     /locus_tag="J1Z28_002200"
     CDS             4263..5246
                     /gene="pheS"
                     /locus_tag="J1Z28_002200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBA7717035.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLHMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5261..7648
                     /gene="pheT"
                     /locus_tag="J1Z28_002201"
     CDS             5261..7648
                     /gene="pheT"
                     /locus_tag="J1Z28_002201"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012905611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBA7717036.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARIYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7653..7952
                     /gene="ihfA"
                     /locus_tag="J1Z28_002202"
     CDS             7653..7952
                     /gene="ihfA"
                     /locus_tag="J1Z28_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBA7717037.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8053..9033
                     /gene="btuC"
                     /locus_tag="J1Z28_002203"
     CDS             8053..9033
                     /gene="btuC"
                     /locus_tag="J1Z28_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBA7717038.1"
                     /translation="MLTLARQQQRQNFRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9095..9646
                     /gene="btuE"
                     /locus_tag="J1Z28_002204"
     CDS             9095..9646
                     /gene="btuE"
                     /locus_tag="J1Z28_002204"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBA7717039.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9646..10395
                     /gene="btuD"
                     /locus_tag="J1Z28_002205"
     CDS             9646..10395
                     /gene="btuD"
                     /locus_tag="J1Z28_002205"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBA7717040.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10473..10937
                     /locus_tag="J1Z28_002206"
     CDS             10473..10937
                     /locus_tag="J1Z28_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase"
                     /protein_id="HBA7717041.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11184..11897
                     /gene="ydiV"
                     /locus_tag="J1Z28_002207"
     CDS             11184..11897
                     /gene="ydiV"
                     /locus_tag="J1Z28_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBA7717042.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTVEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11960..13396
                     /locus_tag="J1Z28_002208"
     CDS             11960..13396
                     /locus_tag="J1Z28_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBA7717043.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGENLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLDDEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13400..13591)
                     /gene="hemP"
                     /locus_tag="J1Z28_002209"
     CDS             complement(13400..13591)
                     /gene="hemP"
                     /locus_tag="J1Z28_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBA7717044.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13723..14769)
                     /gene="aroH"
                     /locus_tag="J1Z28_002210"
     CDS             complement(13723..14769)
                     /gene="aroH"
                     /locus_tag="J1Z28_002210"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBA7717045.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(14926..15759)
                     /gene="ppsR"
                     /locus_tag="J1Z28_002211"
     CDS             complement(14926..15759)
                     /gene="ppsR"
                     /locus_tag="J1Z28_002211"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBA7717046.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16092..18470
                     /gene="ppsA"
                     /locus_tag="J1Z28_002212"
     CDS             16092..18470
                     /gene="ppsA"
                     /locus_tag="J1Z28_002212"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBA7717047.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDSFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18527..20227)
                     /gene="fadK"
                     /locus_tag="J1Z28_002213"
     CDS             complement(18527..20227)
                     /gene="fadK"
                     /locus_tag="J1Z28_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416216.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBA7717048.1"
                     /translation="MHPTGPHLGPDVLFRESKMKVTLTFNEQRRAAYRQQGLWGDASL
                     ADYWQQTARAMPDKIAVVDNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPG
                     WCEFTVIYLACLKTGAVSVPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLIL
                     PLQNQLPQLQQIVGVDKLAPATSSLSLSQILADNTPLTTAITVHGDELAAVLFSSGTE
                     GLPKGVMLTHNNILASERAYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARS
                     VLLDIFTPDACLALLEQQRCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIP
                     KKVARECQQRGIKLLSVYGSTESSPHAVVNLDDPLPRFMHTDGYAAAGVEIKVVDDTR
                     KTLPPGCEGEEASRGPNVFMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGR
                     KKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLS
                     LEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSGKIQKFLLRKDIMRRLTQDVCEEIE
                     "
     gene            complement(20230..20523)
                     /locus_tag="J1Z28_002214"
     CDS             complement(20230..20523)
                     /locus_tag="J1Z28_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBA7717049.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPNINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(20520..21809)
                     /locus_tag="J1Z28_002215"
     CDS             complement(20520..21809)
                     /locus_tag="J1Z28_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7717050.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPIERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASNPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLSDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21864..22802)
                     /locus_tag="J1Z28_002216"
     CDS             complement(21864..22802)
                     /locus_tag="J1Z28_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBA7717051.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTRDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLNR"
     gene            complement(22822..23586)
                     /locus_tag="J1Z28_002217"
     CDS             complement(22822..23586)
                     /locus_tag="J1Z28_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBA7717052.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLDTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSVIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23902..>24429
                     /locus_tag="J1Z28_002218"
     CDS             23902..>24429
                     /locus_tag="J1Z28_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBA7717053.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYI"
BASE COUNT         6003 a         5914 c         6440 g         6072 t
ORIGIN      
        1 ggcgcgcaaa ttgattaata ggctctgatg aaggcgttag tatgtcgcta tcgatgtgca
       61 ttctaagagg agactcataa tgagttctga taatgatatc gtttttactc ataatctatt
      121 cgccttatat atttctggag attttgagga tgatgattga ttattatcgc cagaacgaaa
      181 tgcgcacgag aaattgctat aagaattatg ttacttttat cattgcgata tgactacttc
      241 gtcattaatc tcatgatcgt ttaaatacta ccgccaaatc acgcaacgaa ctttgctgga
      301 ttcagtttat gttgctgtaa atccgctcgt agtaaacctt tcagaagctc tgtgatgtta
      361 tcagtggttc tttaagcgtt ttgctggtgt actcactaca aacgaattgc gaatcgaatc
      421 aatgtgaaac ggaaaggtac aatctccctt tttttagtgt taacatcgtt cgaccgggtg
      481 gaaggtgaga ggttaatgtc tttcatttgt gtacctgatt gcgaaccaat ttagcatttg
      541 ttggctagat ggtttcgcaa tgaactgtta ataaacaaat ttttctttgt atgtgatctt
      601 tcgtgtgggt caccactgca aataaggata taaaatgcct gttataactc ttcctgatgg
      661 cagccaacgc cattacgacc acgctgtaag ccccatggat gttgcgctgg acattggtcc
      721 aggtctggcg aaagcctgta ttgcagggcg cgttaatggt gaactggttg atgcttgcga
      781 tctgattgaa aacgacgcac aactgtcgat cattactgcc aaagacgaag aaggtctgga
      841 gatcattcgt cactcctgtg cgcacctgtt agggcacgcg attaaacaac tttggccgca
      901 taccaaaatg gcaatcggcc cggttattga caacggtttt tattacgacg ttgatcttga
      961 ccgcacgtta acccaggaag atgtcgaagc actcgagaag cggatgcatg agcttgctga
     1021 gaaaaactac gacgtcatta agaagaaagt cagctggcat gaagcgcgtg aaactttcgc
     1081 caaccgtggg gagagctaca aagtctccat tcttgacgaa aacatcgccc atgatgacaa
     1141 gccaggtctg tacttccatg aagaatatgt cgatatgtgc cgcggtccgc acgtaccgaa
     1201 catgcgtttc tgccatcatt tcaaactaat gaaaacggca ggggcttact ggcgtggcga
     1261 cagcaataac aaaatgttgc aacgtattta cggtacggcg tgggcagaca aaaaagcgct
     1321 taacgcttac ctgcaacgcc tggaagaagc cgcgaaacgc gaccaccgta aaatcggtaa
     1381 acagctcgac ctgtaccata tgcaggaaga agcgccgggt atggtattct ggcacaacga
     1441 cggctggacc atcttccgtg aactggaagt gtttgttcgt tctaaactga aagagtacca
     1501 gtatcaggaa gttaaaggtc cgttcatgat ggaccgtgtc ctgtgggaaa aaaccggtca
     1561 ctgggacaac tacaaagatg caatgttcac cacgtcttct gagaaccgtg aatactgcat
     1621 taagccgatg aactgcccgg ggcacgtaca aattttcaac caggggctga agtcttatcg
     1681 cgatctgccg ctgcgtatgg ccgagtttgg tagctgccac cgtaacgagc cgtcaggttc
     1741 gctgcatggc ctgatgcgcg tgcgtggttt tacccaggat gacgcgcata tcttctgtac
     1801 tgaagaacaa attcgcgatg aagttaacgg atgtatccgt ttagtctatg atatgtacag
     1861 cacttttggc ttcgagaaga tcgtcgtcaa actctccact cgtcctgaaa aacgtattgg
     1921 tagcgacgaa atgtgggatc gtgctgaggc ggacctggcg gttgcgctgg aagaaaacaa
     1981 catcccgttt gaatatcaac tgggtgaagg cgctttctac ggtccgaaaa ttgaatttac
     2041 cctgtatgac tgcctcgatc gtgcatggca gtgcggtaca gtacagctgg acttctcttt
     2101 gccttctcgt ctgagcgctt cttatgtagg cgaagacaat gaacgtaaag taccggtaat
     2161 gattcaccgc gcaattctgg ggtcgatgga acgtttcatc ggtatcctga ccgaagaatt
     2221 tgctggtttc ttcccgacct ggcttgcgcc agttcaggtt gttatcatga atattaccga
     2281 ttcacagtct gaatacgtta acgaactgac gcaaaaacta tcaaatgcgg gcattcgtgt
     2341 taaagcagac ttgagaaatg agaagattgg ctttaaaatc cgcgagcaca ctttgcgtcg
     2401 cgtcccatat atgctggtct gtggtgataa agaggtggaa tcaggcaaag ttgccgttcg
     2461 cacccgccgt ggtaaagacc tgggaagcat ggacgtaaat gaagtgatcg agaagctgca
     2521 acaagagatt cgcagccgca gtcttaaaca attggaggaa taaggtatta aaggcggaaa
     2581 acgagttcaa acggcgcgcc ctaaccgtat caatggcgaa attcgcgccc aggaagttcg
     2641 cttaacaggt ctggaaggcg agcagcttgg tattgtgagt ctgagagaag ctctggagaa
     2701 agcagaagaa gccggagtag acttagtcga gatcagccct aacgccgagc cgccggtttg
     2761 tcgtataatg gattacggca aattcctcta tgaaaagagc aagtcttcta aggaacagaa
     2821 gaaaaagcaa aaagttatcc aggttaagga aattaaattc cgtcctggta cagatgaagg
     2881 cgactatcag gtaaaactcc gcagcctgat tcgctttctc gaagagggtg ataaagccaa
     2941 aatcacgctg cgtttccgcg gtcgtgagat ggcgcaccag caaatcggta tggaagtgct
     3001 taatcgcgtg aaagacgatt tgcaagaact ggcagtggtc gaatccttcc caacgaagat
     3061 cgaaggccgc cagatgatca tggtgctcgc tcctaagaag aaacagtaag gccttcaagt
     3121 agcactgtct gtggagcctt tgggcttcac aggcattgtt cgcctttgtt tcgtttatta
     3181 acaatgcgaa gtggaagtta ttaaaatgcc aaaaattaag accgtacgcg gtgctgctaa
     3241 gcgcttcaaa aaaaccggta aaggtggttt taagcacaag cacgctaacc tgcgtcacat
     3301 tctgaccaaa aaagcgacca aacgtaaacg tcacctgcgt ccgaaagcca tggtttccaa
     3361 aggcgatctg ggcctggtaa tcgcgtgcct gccgtacgca taagccgtta acgtttttaa
     3421 ctttttaatt agaatataga tacaggagag cacatatggc tcgcgtaaaa cgtggtgtta
     3481 ttgcacgcgc acgtcacaag aaaattttga aacaagctaa aggctactac ggtgcgcgtt
     3541 ctcgcgtata ccgcgttgcc ttccaggctg ttatcaaagc tggtcagtat gcttaccgcg
     3601 accgtcgtca acgtaagcgt cagttccgtc aactgtggat tgcgcgtatc aacgcagcag
     3661 cacgtcagaa cggtatttct tacagcaaat tcatcaatgg cctgaaaaaa gcctctgttg
     3721 aaatcgaccg taagatcctg gctgatatcg cagtattcga caaagtagcg ttcaccgctc
     3781 tggttgaaaa agcgaaagca gctctggcat aagccagttg aaagagggag ctagtctccc
     3841 tcttttcgtt tcaacgccat caaaacattg acttttatcg ccgtagcctt ttcaataaag
     3901 gtcttttgaa gagtaccaaa aggtaacgca agcaatgaat gctgctattt tccgcttctt
     3961 tttttacttt agcacctgac tccaggaggc tagcgcgtga gaagagaaac ggaaaacagc
     4021 gcctgaaagc ctcccagtgg aggctttttt tgtatgcgcg ttttgaaaaa taaaaagcct
     4081 gtctcatcag gcgtaattgt cgcagccagt ctggacacgg ccagcgcgga gaaaccggag
     4141 cgtacttaag tacgtgagga tttcgagcac tgcccgggac caaaatggca agtaaaatag
     4201 cctgatggga tgggctctaa gtccaacgaa ccagtgtcac cactgacaca atgaggaaaa
     4261 acatgtcaca tctcgcagaa ctggttgcca gtgcgaaggc ggccattagc caggcgtcag
     4321 atgttgccgc gttagataat gtgcgcgtcg aatatttggg taaaaaaggg cacttaaccc
     4381 ttcatatgac gaccctgcgt gagctgccgc cagaagagcg tccggcagct ggtgcggtta
     4441 tcaacgaagc gaaagagcag gttcagcagg cgctgaatgc gcgtaaagcg gaactggaaa
     4501 gcgctgcact gaatgcgcgt ctggcggcgg aaacgattga tgtctccctg ccgggtcgtc
     4561 gcattgaaaa cggcgggctg catccggtga cccgtaccat cgaccgtatc gaaagtttct
     4621 tcggtgagct tggctttacc gtggcaaccg ggccggaaat cgaagacgat tatcataact
     4681 tcgatgctct gaacattcct ggtcaccacc cggcgcgcgc tgaccacgac actttctggt
     4741 ttgacgctac ccgcctgctg cgtacccaga cctctggcgt acagatccgc accatgaaag
     4801 cccagcagcc accgattcgt atcatcgcgc ctggccgcgt ttatcgtaac gactacgacc
     4861 agactcacac gccgatgttc catcagatgg aaggtctgat tgttgatacc aacatcagct
     4921 ttaccaacct gaaaggcacg ctgcacgact tcctgcgtaa cttctttgag gaagatttgc
     4981 agatccgctt ccgtccttcc tacttcccgt ttaccgaacc ttctgcagaa gtggacgtca
     5041 tggggaaaaa tggtaaatgg ctggaagtac tgggctgcgg gatggtgcat ccgaacgtgc
     5101 tgcgtaacgt tggcatcgac ccggaagttt actctggttt cgccttcggg atggggatgg
     5161 agcgtctgac catgttgcgt tacggcgtca ccgacctgcg ttcattcttc gaaaacgatc
     5221 tgcgtttcct caaacagttt aaataaggca ggaatagatt atgaaattca gtgaactgtg
     5281 gttacgcgaa tgggtgaacc cggcgattga tagcgacgcg ctggcaaatc aaatcacgat
     5341 ggcgggcctg gaagttgatg gtgtagaacc ggttgccggt agcttccacg gcgtggtcgt
     5401 tggtgaagtg gttgagtgtg cgcagcatcc gaacgctgac aaactgcgtg tgacaaaagt
     5461 gaatgtcggc ggcgatcgcc tgctggacat cgtctgcggt gcgccaaact gccgtcaggg
     5521 cctgcgtgtg gcggtagcga ccattggtgc tgttctgccg ggtgatttca aaattaaagc
     5581 cgcgaaactg cgtggcgaac cgtctgaagg gatgctgtgc tccttctctg agctgggaat
     5641 ttctgacgat cataacggca ttatcgagct gcctgcggat gcgccgattg gcaccgacat
     5701 ccgcgaatac ctgaaactcg atgacaacac catcgaaatt agcgtgacgc caaaccgtgc
     5761 cgactgctta ggtatcattg gtgttgcgcg cgacgttgcc gtgttgaacc agctgccgct
     5821 ggttgaaccg gaaatcgttc cggttggtgc gaccatcgac gacacgctgc cgattgcagt
     5881 agaagcgccg gatgcctgcc cgcgctatct tggccgtgtg gtaaaaggca ttaacgttaa
     5941 agcgccaact ccactgtgga tgaaagaaaa actgcgtcgt tgcgggatcc gttctatcga
     6001 tgcagttgtt gacgtcacca actatgtgct gctcgaactg ggccagccga tgcacgcttt
     6061 cgataaagat cgcattgaag gcggcattgt ggtgcggatg gcgaaagagg gtgaaacgct
     6121 ggtattgctc gatggtactg aagcgaagct gaatgctgac accctggtca tcgccgacca
     6181 caacaaggcg ctggcgatgg gcggcatctt tggtggcgaa cactctggcg tgaatgacga
     6241 aacgcaaaac gtcctgctgg aatgcgcttt cttcagcccg ctgtctatca ccggtcgtgc
     6301 tcgtcgtcat ggcctgcata ctgatgcgtc tcaccgttat gagcgtggcg ttgatccggc
     6361 actgcagcac aaagcgatgg aacgtgcgac ccgtctgctg atcgacatct gcggtggtga
     6421 agcgggtccg gtaattgata tcactaacga agcaacgctg ccgaagcgtg caaccatcac
     6481 tttacgtcgt agcaaactgg atcgcctgat cggccatcat attgctgatg agcaggtaac
     6541 agacattctg cgtcgtctcg gctgcgaagt gaccgaaggc aaagacgagt ggcaggcagt
     6601 tgcgccgagt tggcgtttcg acatggagat tgaagaagat ctggtcgaag aagtcgcgcg
     6661 tatttacggc tacaacaaca tcccggatga gccggtacag gcaagcctga ttatggggac
     6721 tcaccgtgaa gctgacctgt cgctcaagcg cgtaaaaacg ctgctcaacg acaaaggcta
     6781 tcaggaagtg atcacctata gcttcgttga tccgaaagtg cagcagatga tccatcctgg
     6841 cgttgaagcc ttactgctgc caagcccgat ctctgttgaa atgtcagcaa tgcgtctttc
     6901 cctgtggacc ggcctgctgg caaccgtggt gtacaaccag aaccgtcagc agaatcgtgt
     6961 acgcattttc gaaagcggtc tgcgcttcgt gccagatact caggcaccgt tgggtattcg
     7021 tcaggatctg atgttagccg gtgtgatttg cggtaaccgt tacgaagagc actggaacct
     7081 ggcaaaagag accgttgatt tctatgattt gaaaggcgat cttgaatccg ttctcgacct
     7141 gaccggtaaa ctgaatgagg ttgagttccg tgcagaagcg aatccggcac tgcatccggg
     7201 gcaatccgca gcgatttatc tgaaaggtga acgtattggt tttgttgggg ttgttcatcc
     7261 tgaactggaa cgtaaactgg atctcaacgg tcgcactctg gtgttcgaac tggagtggaa
     7321 caagctcgca gaccgcgtgg tgcctcaggc gcgcgagatt tctcgcttcc cggcgaaccg
     7381 tcgtgacatc gcggtggtgg tcgcagaaaa cgttcccgca gcggatattt tatccgaatg
     7441 taagaaagtt ggcgtaaatc aggtagttgg cgtaaactta tttgacgtgt accgcggtaa
     7501 gggtgttgcg gaggggtata agagcctcgc cataagcctg atcctgcaag ataccagccg
     7561 tacactcgaa gaagaggaga ttgccgctac cgtcgccaaa tgtgtagagg cattaaaaga
     7621 gcgattccag gcatcattga gggattgaac ctatggcgct tacaaaagct gaaatgtcag
     7681 aatatctgtt tgataagctt gggcttagca agcgggatgc caaagaactg gttgaactgt
     7741 ttttcgaaga gatccgtcgc gctctggaaa acggcgaaca ggtgaaactc tctggttttg
     7801 gtaacttcga tctgcgtgat aagaatcaac gcccgggacg taacccgaaa acgggcgagg
     7861 atattcccat tacagcacgg cgcgtggtga ccttcagacc cgggcagaag ttaaaaagcc
     7921 gggtcgaaaa cgcttcgccc aaagacgagt aatctgatct aactaaaaag gccgctctgc
     7981 ggcctttttt cttttcactg tcgaagagtc accgtaaaat caacgccatg acacttcagc
     8041 agaacggata ccatgctgac acttgcccgc caacaacagc gacaaaattt tcgctggtta
     8101 ttatgcctgt cagttttgat gctgctggcg cttctcttaa gcctttgcgc cggtgaacta
     8161 tggattttgc caggtgactg gttttctcct cgtggcgaac tgttcgtctg gcaaattcgc
     8221 ctgccacgta cgctggctgt attgctggtt ggtgcggcgc tggctatatc cggcgctgta
     8281 atgcaggcgt tgtttgaaaa tcctctggca gaacctggac tacttggcgt ctctaacggc
     8341 gcaggcgtgg ggcttatcgc cgcggtattg cttgggcaag ggcaactccc caactgggcg
     8401 ctagggctgt gtgcgattgc tggcgcgctt atcatcactt taatactctt acgtttcgct
     8461 cgtcgtcatc tttcgaccag tcggttattg ctggctggcg ttgcattagg gattatctgt
     8521 agcgcactaa tgacgtgggc tatctacttt tccacctctg ttgatttacg tcagctgatg
     8581 tactggatga tgggcggttt tggcggcgta gactggcggc aaagctggct gatgctggca
     8641 ttgatccccg tgttgttgtg gatctgttgt cagtccaggc cgatgaatat gttagcactt
     8701 ggcgagatct cggcgcggca actgggttta cccctgtggt tctggcgcaa tgtgctggtg
     8761 gcagcgaccg gctggatggt tggcgtcagt gtggcgctgg cgggtgctat cggctttatt
     8821 ggtctggtga tcccacatat tctccggctg tgtggtttaa ccgatcatcg cgtattactt
     8881 cccggctgcg cgctggcagg ggcgagcgca ttgctgctgg ccgatattgt agcgcgcctg
     8941 gcattagctg ccgcagagct gccaattggc gtggtcaccg caacgttggg tgcgccggtg
     9001 tttatctggt tattgttaaa agcaggacgt tagccgcaaa agacggtcta tgattaaaag
     9061 ctaagatttt tactgaccac acccaagaga aacgatgcaa gattccattc tgacgaccgt
     9121 agtgaaagat atcgacggtg aagtgaccac gctggagaag tacgccggta atgtgctgtt
     9181 gattgtcaat gtcgcctcaa agtgtggctt aacgccgcaa tatgagcagt tggagaatat
     9241 tcagaaagcc tgggctgatc gtggttttgt ggtgctggga ttcccgtgta accagtttct
     9301 ggagcaagaa ccgggtagcg atgaagagat taaaacttac tgcaccacca catggggggt
     9361 gacgttcccg atgttcagta agattgaagt taatggcgaa ggacgccatc cgctgtatca
     9421 aaaattgatt gccgcagcgc cgactgcagt cgcgccggaa gagagcggat tctatgcccg
     9481 tatggtcagc aaaggtcgtg caccgctgta ccctgatgat attttatgga attttgagaa
     9541 atttttagtt ggcagggacg gattggtcat ccagcgtttt tccccggata tgacgccgga
     9601 agatcccatt gtgatggaaa gcattaaact ggcgttggca aaataatgtc tattgtgatg
     9661 cagttacagg atgttgcgga atctacccgc ctggggccgc tttctggcga ggttcgggct
     9721 ggggagatcc tgcacctggt ggggccgaat ggcgcgggta agagtacctt actggcgcga
     9781 atggccggaa tgaccagcgg taagggaagc attcagttcg cggggcaacc actggaagca
     9841 tggtctgcaa caaagctcgc gctgcatcgc gcctatcttt cacaacagca gacgccgccg
     9901 tttgcaatgc cggtctggca ctacctgaca ctgcatcagc acgataaaac gcgtaccgaa
     9961 ctactgaatg atgtcgcagg ggcgctggct cttgatgaca aactcggacg tagcaccaat
    10021 caactttccg gcggtgaatg gcaacgcgta cgtcttgctg cggtggtgct gcaaatcaca
    10081 ccacaagcca atcccgcagg ccaattgctg cttcttgatg agccgatgaa cagtcttgat
    10141 gttgcgcaac aaagcgcctt agacaaaatt ctgagcgcgc tgtgtcagca aggactggcg
    10201 attgtgatga gcagtcacga tctcaaccac acattgcgtc atgcgcatcg ggcgtggttg
    10261 ctaaaaggtg gaaaaatgct ggccagtgga cgcagagaag aggtgctcac gccggcaaat
    10321 ctggcgcagg cctatgggat gaattttcgc cgtctggata tcgaaggtca cagaatgctg
    10381 atttcgacca tctgatttca ttagtatctt gaaaatcggg taattacgac gctaaattaa
    10441 tgcaagaata aaaaacagag gaaccgtacg gaatgcgttt ctgccttatt ttgatcacag
    10501 cactgtttct ggccgggtgt agccaccata aagcaccgcc gccaaatgcc agactttctg
    10561 attcgattac cgttattgcc ggtttgaacg accagctaca aagctggcat ggcacgccgt
    10621 atcgttatgg tggcatgacg cggcgcggtg tggactgttc gggatttgtg gttgtgacga
    10681 tgcgcgatcg tttcgatttg cagctgcccc gtgaaaccaa gcaacaagcc tctattggca
    10741 cgcaaattga taaagacgag ttgctgccag gtgacctggt ctttttcaaa acgggttccg
    10801 gacaaaatgg tttgcatgta ggtatttacg ataccaacaa ccaatttatc cacgcctcta
    10861 ccagcaaggg agtgatgcgt tcctcacttg ataatgtcta ttggcagaaa aatttctggc
    10921 aggcgagacg aatctagtat gtcgaaaaat ggacggcgat gatacatcgc cgtccattta
    10981 ctcctgaata cagtagtatt ttgcagacaa aaattccgta aatggattat tcgtttataa
    11041 tcaggataat ctaaaaaaag gacccctgaa aaagtggtta acatggaaca aatgaaagtt
    11101 actccttctg taattgttat gtcttatggc ctcatccctt ttagccggat actgaaaaac
    11161 atccttcgag agggacggtt accatgaaga tttttttgga gaatctttat cattctgatt
    11221 gctatttcct gccgataaga gataaccagc aagaccttgt cggcgttgag ttaattaccc
    11281 atttctccag tgaagatggt acggttcgta tcccgacgag tcgggttata gcgcaactta
    11341 ccgtggagca acattggcaa ttattttctg aacagttgga attactaaaa tcatgccaac
    11401 acttttttat tcaacataaa ctctttgcct ggcttaattt aacgccacaa gtggctacat
    11461 tattattaga cagggataat ttcgcaggcg aactattaaa atatccgttt attgaactgt
    11521 tgattaatga gaattacccg catttcaatg aaggaaaaga taatcgcgat cttttatcct
    11581 tatcgcaaat gtatcccctt gtgttaggta atttgggagc aggtaatagc acaatgaaag
    11641 cggtttttga tggtcttttc acccgcgtca tgctggataa aagttttatt cagcaacaaa
    11701 tcacgcatcg ctccttcgaa ccgtttattc gtgcgatcca ggcgcaaatt tccccttgtt
    11761 gcaattgcat catcgcgggg ggcattgata cgccagaaat attggcacag attatcccct
    11821 ttgatttcca tgctctccag ggatgcctgt ggcctgcagt tcctataaat cagataacga
    11881 cgttagttca gcgataaccc tcctgtttgc tggtgtttag gacgagagta accgtctaca
    11941 ctatcaaaca ggaggatcta tgaccctgtc ttttattacc cgctggcgcg atgaattgcc
    12001 agaaacctat acagcacttt cccctacgcc tttaaataat gcccggctga tttggcataa
    12061 taccgaactg gctaacacgc tgagtattcc atcgtcgctg tttaaaaatg gcgcaggtgt
    12121 ctggggcggc gaaaacctac tgcctggtat gtcaccactg gcgcaggttt acagtggtca
    12181 tcagtttggc gtctgggcgg gccaactggg tgatgggcgc ggcattttac tcggcgaaca
    12241 actgcttgct gatggcacta caatggactg gcatctgaaa ggtgctggcc tgacgcctta
    12301 ttcgcgaatg ggtgatggac gggcggtttt acgttcgacg atacgagaaa gtctcgccag
    12361 tgaggcgatg cattatctgg gcattccgac gacccgcgct ttaagtatcg tcaccagcga
    12421 ttcgccagtg tatcgggaaa cggtggagtc aggcgcgatg ctgatgcgtg tggcaccaag
    12481 tcatctgcgc tttggtcatt tcgaacattt ttactatcgc cgcgagccgg aaaaggttcg
    12541 tcagttagct gactttgcca ttcgtcatta ctggtcacat cttgatgatg aggaggacaa
    12601 ataccgtctc tggtttaccg atgttgtcgc acgtaccgcc tcgttaattg cccaatggca
    12661 gacggtcggc tttgctcatg gggtgatgaa taccgacaac atgtcgctgc tggggctgac
    12721 gcttgattac gggccgtttg gttttcttga tgattacgaa cccggtttta tttgtaatca
    12781 ctcggatcat caagggcgtt acagctttga taatcaacct gccgtcgcgt tgtggaattt
    12841 acagcgtctg gcgcagacat tgtcgccatt tgttgccgta gatggcctga atgaggccct
    12901 ggacagctat cagcaggttt tgttgacgca ttatggacaa cggatgcggc agaaactggg
    12961 cttcatgacg gagcaaaaag aggataacgc gctacttaat gaattattca gtctgatggc
    13021 gcgagagcgc agcgattata cccgcacatt ccgcatgctg agtctgaccg agcagcacag
    13081 cgcggcgtca ccgctacgtg atgagtttat tgatcgtgcg gcatttgatg actggtttgc
    13141 ccgttatcgg gggcgtttgc aacaagacga gattaccgat agtgagcgtc agcaactgat
    13201 gcaaagcgtt aacccggctc tggtgttgcg caactggttg gcgcaacggg cgattgaagc
    13261 ggcagaaaag ggtgatatga cggaattgca ccgcctgcat gaggcgttgc gaaatccttt
    13321 cagcgacaga gatgatgact atgtcagtcg tccacccgac tggggtaaac ggctggaagt
    13381 cagctgctcg agttaaaggt tacttggtca acagcagctt gccagcctga gttttacgga
    13441 gcagatattc ttgcccgtca tgatcgataa tcagtttgcc atccggacct aacagtgtct
    13501 ggctggtaat tcgccggatg ggctgcgggg aagcggtctt gtgattggca tccgtttcag
    13561 gcgtgagttt tctgctatcc gtataacgca taaaaattaa taataattta tataacaatg
    13621 attcttatta tcaatattat ggcgggccag agcaagcagt aatttgttga gaagacaaca
    13681 agttgcgcag aaatgcccat cccgtcagga atgggcacgc attcagaagc gagtatctac
    13741 cgcagaggcg agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt
    13801 aatggattga ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag
    13861 gaaactttcc gccataattc cagcaatcgc cgtagagcca ttgcggattt gctgacaaat
    13921 atcctcacaa acttctaact ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc
    13981 caccaccaga tgttcaggta aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc
    14041 atcggcatga taattcggtt ttttgccgcc acgcataata atgtggccat acgggttgcc
    14101 gctggtctga tagatggtca tctgaccgtt tttgtctggt gagaggaaca tatggctggc
    14161 gcgggctgcg cggatagcat ccacggcaat ccgcgtgttg ccatcggtac catttttaaa
    14221 acccaccgga caggagagtg ccgaagccat ttcgcggtgg atctgacttt cggtagtacg
    14281 tgcgccaatc gcgccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag
    14341 gaactcagtc gcggtaggga cgcccagctc atttacctgt aaaagtaatt tgcgcgccag
    14401 ctccagaccg tgatttaccc gatagctacc gtttaaatct ggatcggaga tgagtccttt
    14461 ccagccgaca accgttcgtg gtttttcaaa ataggtgcgc attacgattt ccagccgtgg
    14521 ctggtactgg ttgcgtagcg actgcagacg ggtggcgtac tccatcgcag cggtgagatc
    14581 gtggatcgag caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt
    14641 ttcaattcta cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg
    14701 tagcgcgagt tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt
    14761 tctgttcatt acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat
    14821 gcgcatcacg ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg
    14881 ggtggttagc atgataacaa aaaagtaagt caatgcacta gttctctagt acattcggcg
    14941 gctaaggccc atgatatcga ggatcttggt ggcaatctct tctaccgaat aattggtact
    15001 gttaatccac gggatctgat ttttacggta caacgcttcc acttccgcga cttccatcct
    15061 gcactgacga agcgaggcat agcgactgtt ctcccgacgt tcctcgcgaa tcgccgccag
    15121 gcgttccggg tcgatagtca ggccgaacaa tttgtgctga agtggtttga gcgacgcggg
    15181 tagcaccaga ttatccatat cgtcggcaat aaaggggtaa tttgccgcgc ggataccaaa
    15241 ttgcattgcc agatacagac tggtgggggt tttaccacag cgagaaacac cgagcaggat
    15301 cacctgagcc tggtccagat tgcgcaacga aataccgtca tcgtgggcaa gggtgtaatc
    15361 aatcgccgca atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg
    15421 atgagcaatc ggcgttggat ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg
    15481 aacgatatcc tggcaaaagc cttcactttg caagatgata gcgcgaatct ccggcaacac
    15541 gatggagtag aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc
    15601 cttcactgcc cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt
    15661 gacgggaaat tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc
    15721 agaaatataa aaaacgtggc gatcaacagc attatccatt ttgttcttcc cgtgatgcag
    15781 acataattct atgaaagcat aaattaaaaa cgcacagaag cgtagaacgt tatgtctggt
    15841 ttataaaatg aaacttaaat tttatttttt atgaaaacag catttcattt ttatggtttc
    15901 gtttataccg atggtttatg tggaaattgt cgaagagagc agatttgcgc aacgctggga
    15961 tcagtcttaa aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg
    16021 ttaaatatgc aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa
    16081 aggattgttc gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg
    16141 gcatgaatga tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta
    16201 acctttccgg aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta
    16261 accagtttct ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg
    16321 atattgacga tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg
    16381 acactccctt ccagcctgag ctggaaaacg ccatccgcga agcctatgca cagctttccg
    16441 ccgatgacga aaacgcctct tttgcggtgc gctcctccgc caccgcagaa gatatgccgg
    16501 acgcttcttt tgccggtcag caggaaactt tcctcaacgt tcagggtttt gacgccgttc
    16561 tcgtggcagt gaagcatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg
    16621 tgcaccaggg ttacgaccat cgtggcgtag cgctctccgc cggtgttcag cggatggtgc
    16681 gctccgacct cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc
    16741 aggtggtgtt tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggtgcggtta
    16801 acccggatga gttttatgtg cataaaccga cacttgcggc gaatcgcccg gctatcgtgc
    16861 gccgcaccat ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca
    16921 agcaggttaa aatcgaagac gtaccgcagg aacagcgtga catcttctcg ctgaccaacg
    16981 aagaagtgca ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccga
    17041 tggatattga gtgggcgaaa gatggccaca ccggcaaact gttcattgtg caggcgcgtc
    17101 cggaaaccgt gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta
    17161 agattatcgc cgaaggccgt gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca
    17221 tccatgacat cagcgaaatg aaccgcatcg aacctggcga cgtgctggtt actgacatga
    17281 ccgacccgga ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg
    17341 gtcgtacctg tcacgcggcg atcatcgctc gtgaactcgg cattccggcg gtagtgggct
    17401 gtggtgatgc aacagaacgg atgaaagacg gcgagaacgt cactgtttct tgtgccgaag
    17461 gtgataccgg ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag
    17521 aaacgatgcc ggacctgccg ttgaaggtga tgatgaatgt cggtaacccg gaccgtgctt
    17581 tcgacttcgc ctgcctgccg aacgaaggcg tgggccttgc gcgtctggaa tttatcatca
    17641 accgtatgat tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt
    17701 tgcaaaacga aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg
    17761 gtcgtctgac tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg
    17821 tccgtctttc tgattttaaa tcgaacgaat atgccaatct ggtcggtggt gagcgttacg
    17881 agccagatga agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca
    17941 gcttccgcga cagcttcgcg ctggagtgtg aagcagtgaa acgtgtgcgc aacgacatgg
    18001 ggctgactaa cgttgagatc atgatcccgt tcgtgcgtac cgttgatcag gcgaaagcgg
    18061 tggttgagga actggcgcgt caggggctga aacgtggtga gaacgggctg aaaatcatca
    18121 tgatgtgtga aattccgtcc aacgccttgc tggcagagca gttcctcgaa tatttcgacg
    18181 gcttctcaat tggctcaaac gacatgacac agctggcgct cggtctggac cgtgactccg
    18241 gcgtggtgtc tgaactgttc gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg
    18301 cgattcgtgc cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccatccg
    18361 accacgaaga ctttgctgca tggttgatgg aagaggggat cgatagcctg tctctgaacc
    18421 cggacaccgt ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatacc
    18481 cggcggcgtt ttgtcgccgg gtttatgtga tccccgaaga tgaaacttat tcaatctctt
    18541 cacagacatc ctgagttaat cgccgcataa tatctttcct taacaaaaac ttttgtattt
    18601 tacctgagac agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt
    18661 tcgcgacccg tttacggcta aaaaaagcca ccacctcttc cagcgataat gaatgatgcg
    18721 gcgctttcag cacgacataa gcgcatgatc gttcacctaa gcgttcatcg ggcatcgcaa
    18781 ccacacaggc atcgtgaatt ttaggatgct gtaataaaat atcttccact tcacggctgc
    18841 taatattttc gccgccgcgg acaataatat ctttcttgcg cccggttatt tttatatagc
    18901 cagcctcatc catgcggcag agatcgccgc tgtaatacca gccttcttca tccagcgcac
    18961 gggcggttaa ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgaggctt
    19021 cttcaccttc gcaacctggc ggtaaagtct tgcgtgtgtc atcgaccact ttaatctcta
    19081 cacctgcggc agcgtaaccg tcggtgtgca taaagcgcgg caaaggatcg tcgagattca
    19141 ccaccgcatg cggcgaactt tctgtggaac cataaacact taataattta atgccgcgct
    19201 gctggcattc tcgcgccact tttttgggga ttgtggtacc tccgcaaaga aagaagcgca
    19261 gcgctgaaag gtctgcgggt tgtttctcta gtaaattcaa aagatcatag acaaacggcg
    19321 ttgcgccgag catacaggtg caacgctgct gctcaagcag cgcgagacac gcatcaggag
    19381 tgaaaatatc taacaacacg ctgcgagcac caatcaaaaa aggtgctgtt acgccatgca
    19441 gaaagcccgt tgcgtgacca agtggcgcag gcatcataaa gacatcctgc caggtcagat
    19501 ttagccgcgc gcaataagcc cgctcactgg cgagaatatt gttatgcgtt agcatcacac
    19561 cctttggcag accctcggtt ccggaggaaa atagcacagc ggctaattca tcgccgtgga
    19621 ccgttatcgc cgtggtcaga ggagtattgt cggcgagaat ctgacttaat gagagggaag
    19681 aggtggcggg agccagtttg tccacgccga caatttgttg tagttgtgga agctgatttt
    19741 gcagcggcag gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca
    19801 tttttgcctg acacttattg agcacccaca ccagttctgc ttcccgccag gaaggtaaca
    19861 gcggcacgga aaccgcaccg gttttcaggc aggcaagata gataacggta aattcacacc
    19921 agcctggcag ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt
    19981 ttgccagaca gctcgcggcg tgatcgagcg cgctataggt gtacgttgca ccatgattgt
    20041 cgaccacggc aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg
    20101 aagcatcgcc ccataaccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta
    20161 atgtcacttt cattttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg
    20221 gatgcatgat tagccgtagc gaaattcaat accgaaggtg cctgcgggat attgccactg
    20281 ttcgagaata gtgttgccgc acagcacccg acaggtgccg cactccagac aaccggcgga
    20341 atcgaaatga atgtttcctg cgtcatcctg cttataaagt ccggcagggc aagctttcat
    20401 taatttacgg aattcattga tattgggatt tgccgccaaa atgatatgcg ggtggccctc
    20461 atcaacatgg aatttattga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct
    20521 catagcgcgg ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc
    20581 gcgtgtccca tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacata
    20641 tcgttcatga tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc
    20701 agcgccggga tcttgcggaa atgctgcata tcgcgcataa cacaactttg ttccagctca
    20761 cgtttgtatt gcgccagact gctggcggag aaatccgcgc gttctttggc ggcgatcacc
    20821 gttgtggcgg cagcctgagc tgatgcaatg gctaaatcca tgccgcgaac tgtaaaaccc
    20881 aaattcaggc agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccagctgc
    20941 ggcaccattg ccagaccgcc ctccggcacc atatgcgcgg aatattcaag cagtttaccg
    21001 ccgctaatca gcgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg
    21061 cttttttgcg catgggcgat atcactcaat ccacaaacca gccccaacga tacggaatct
    21121 ttgttggtat agaggaatcc gccgcccatc aggccgtcag aaggggaacc ggcaaacagc
    21181 caggcggcac cttcctcgcc cgtaacatta aagcgatcgt tgatctgttc tggtgtcagg
    21241 cctataacct ctttaacacc aacagcgtaa tgatgcggat tggaagcgga aaccattccc
    21301 agcgagcggc caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga
    21361 atatcatccc cggcctggac gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg
    21421 cgtacgcccg ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc
    21481 agacgattac gcaatacggt ataagacgcg tgttgcggaa catctggttg ctcgcggtga
    21541 aaatcgaggg taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg
    21601 cgttctatcg gcgctgatgc tgcaaagcct ggaataattg cttcgagtgt gtgggcataa
    21661 agacgcccgc cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca
    21721 tccagcccgg ctcgcgccat gaccagtgcg gcaacgctac cggcaacgcc cgcaccgacc
    21781 acaatggcat caaatttgtc atccgacatg cgttatgctc ccttcccgct gcgtttacag
    21841 cgggagatgt ggttgaaagg gaattagcga ttcaactggc taatcagggc agggacgact
    21901 ttgtagatat cgcccaccag accgtagtcg gcatagttga agattggcgc atttttatct
    21961 ttattaatgg cgacaatcac ttttgcgccg ttgccgccaa ccatatgctg gatctgcccg
    22021 gagatcccca gcgtcaggta gagatcggat ttcagcaaca cgccggagac accgatataa
    22081 cgttcacgct ccatccagtt ctcgccttcg gcaattggac gtgaacagcc gacttcagca
    22141 ttcagcaccg ccgccagttc gtggaccatt ttcaggtcat cctgcgccgc cagaccacga
    22201 ccgacgccaa ccacacgttt tgctttgctc aggtccacgc tgcttgccgc tttcgcacgg
    22261 cgttcgcgac agagaatttc atgacgcggg gcaacataag gtaccgtttc tgtcgggcac
    22321 tgatgagaga tatcacgggt gcacggctcc tgaacaccgg gtgcaagggt aatgatcgcc
    22381 agcgggctgt tgatcttttc ctgagcgaac gctaacccgc catacatccg gtgttcggcg
    22441 caaatgtgac catcgacaat atccaccgcc gtggcatcgt tcaccagcgc cgcattcagt
    22501 tgcacactta accgtgctgc cagagcttta ccacgtttcg tcgcggccaa cagcaacata
    22561 gcggggtgtt tatctttcag cagggcagca atgctttcgg cgtaattttc agtacgttgc
    22621 agcgcgtcgt tttgctcaag aacataaatg cattttggcc cataaggcat aactgcttgc
    22681 gcctggtcgg tattttgtac aatggcatac acctgttggc cccattgctg agcgccgcca
    22741 aacagttcag cataacgttc aggattatcg ctaaagaccc agacgctgtt taattgactc
    22801 atcgtttctt ctccgtaggc ttcagttcag ggcttttttc agatgttccg ccagctcggc
    22861 aatggcctcc ggcgaatcgt tatcgagaat gatgtgctta cgttctgttt gcggcggtac
    22921 gcgaataccg gtcagttcag caagtggtgc gctctggctc cagccaatat cacttgcctg
    22981 ccactgattt accggttttt tacccgcgcc gaggatggct ttcatcgaag gaatacgtgg
    23041 tacgttaata tcggaggtga cgcagagcac ggctggaaca gagagttcaa taacttcaac
    23101 atcatcttca agcgtgcgtt caatcaccag tgtattgccc tgacgctgaa taacactcac
    23161 tgcattaatc accggaagtt gcagaatttc tccgaccagc aagccaacct gctgggcata
    23221 aaggtcgccg gaaccttcac caaagatcag taaatcgaag ccgatctttt caactgctgt
    23281 cgccagcgcc tttgcggtat cgagaggcag tgcatgctca agttgtgcat cctgcaccat
    23341 atacaggctg tgcggcccgc gggatagcac gtctttgcgc actttcgagt tctgcaacaa
    23401 agagccgccg acggtcagcg cggctatctc atcgtcatct gttgcaagct ggcttgcagc
    23461 ttcaatggca ttgagatcga actggctgat tttggcgtcg gcattgtcga aattcagggt
    23521 gtattctgga gtgacaacaa tgtcctgttc ttcaggcacc agcttaaagc aggttattat
    23581 tttcatgatt tctcctgtga gtcgcatacg gaaccatctc cgatggaagg taaaaatggc
    23641 ctgaaaatag tgtgcgatag cggtgcgggg ttttcgacgg agcaagctgt tttccttgcc
    23701 aacaatcttg caaaaaaaat gcgatcgcta aaacaaaagt gcaatttgcg gcaatatagt
    23761 tattccatga ccgtgcaatc ttgcattcag tgtaaataat cttgtttagc tctcgctaaa
    23821 cgcggttgta taaccgcaat acacgccgta ttttatccgg catatattgt ttttgtttgc
    23881 tcatggaata gggaggttgt catgtatcaa cgctgttttg ataatgccag tgaaacgctg
    23941 tttgtcgccg gtaaaacgcc acggctttca cgtttcgcat ttagcgatga tccaaaatgg
    24001 gagtctgggc atcacgttca tgacaatgaa accgagctga tttacgtcaa gaaaggggta
    24061 gcaaggttta ccatcgattc ttcgttatat gtcgcacatg ccgatgacat tgtggtgata
    24121 gaacgtggca ggttgcatgc ggtggcctct gacgttaacg atccggcaac gacgtgtacc
    24181 tgtgcgctgt acggctttca gtttcagggg gctgaggaaa atcagctact gcaaccgcat
    24241 tcttgcccgg taattgccgc agggcaggga aaagaagtca ttaaaacctt atttaatgag
    24301 ctaagtgtga ttttgccgca aagtaaaaat agccaaacat cttcgttatg ggacgcattt
    24361 gcctatacgt tagcaattct ttactacgaa aactttaaaa atgcttatcg ttcggagcag
    24421 ggatatatt
//
LOCUS       DADSZD010000063        23949 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.063, whole genome shotgun sequence.
ACCESSION   DADSZD010000063 DADSZD010000000
VERSION     DADSZD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.063"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(44..301)
                     /locus_tag="J1Z28_002219"
     CDS             complement(44..301)
                     /locus_tag="J1Z28_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717054.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDVIANRAPIGQW
                     EPYSEESLLGIIVTSVCRIKVAMLKPEPPRDPHIPLMGDFN"
     gene            291..527
                     /locus_tag="J1Z28_002220"
                     /pseudo
     CDS             291..527
                     /locus_tag="J1Z28_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            615..853
                     /locus_tag="J1Z28_002221"
                     /pseudo
     CDS             615..853
                     /locus_tag="J1Z28_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1095..2426
                     /locus_tag="J1Z28_002222"
                     /pseudo
     CDS             1095..2426
                     /locus_tag="J1Z28_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375825.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2440..3308
                     /locus_tag="J1Z28_002223"
                     /pseudo
     CDS             2440..3308
                     /locus_tag="J1Z28_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3402..3764
                     /gene="queD"
                     /locus_tag="J1Z28_002224"
     CDS             3402..3764
                     /gene="queD"
                     /locus_tag="J1Z28_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBA7717055.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPDGHKCGRLHGHSFVVRLEITGEV
                     DKNTGWIIDFAELKAIFKPTLDRLDHYYLNDIPGLENPTSEVLAEWIWKQIKSQLPHL
                     TAVMVKETCTAGCIYRGE"
     gene            4220..7327
                     /locus_tag="J1Z28_002225"
     CDS             4220..7327
                     /locus_tag="J1Z28_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase SNF2"
                     /protein_id="HBA7717056.1"
                     /translation="MNVLPIGSLVISEQFEGIGKVVTVDSDTNNATVAFFESPAQPYA
                     RQMKVPLEQLTLTIPHEETVIYCIEPHSQRWTRARFGGSRPKGDFLVIFREDETTTLP
                     IDEIFVLNKAPDTPINPADFLALQANDAPFFFPYRQAFIETYIQQRAACRAMASISSS
                     AVELEPHQLAVVRRVLQDKNPKYILADEVGLGKTIEAGMVIREHALEATGHVSMLIAV
                     PAPLVSQWREELAERFQLKQLIIDASTALAGLRQNEATEGIVICSHCDGCTLIERGFT
                     PSLIAVDEVHQIASWPWSGDKDERYDFNLIAEGCRKAHYVLLLTGTPLHGHERNFLSM
                     LHCINPEAYQVDETHLQDFTELVKNRENLGGIFSGLVPSVSNVSLRRNLETLQQQFPE
                     DTVLMTLCQQLIPLIGTFSPNTPEREEGIRDIRRHLGENYRIHHRLLRNRRQVSLSFD
                     ETKNLNLNLLFPGLNGAEECRWYCDGLALDELIDEYRSLSLLPQNTQWAMSEDTYLFW
                     VDDLLSSPLCVMQRAKVHLGEAERTQEERDLLEHIVSTAKNELQAIDYKLAITLNEWL
                     QKNPTGKAIVFCDRPDLAQHLAIKLELLLEYPQELYQPGHPLMYLKPGSPIRILICDK
                     SGEDGLNLHGGLRLAVHYGLPRSCSRIEQRLGRLNRYSANLKGVKPVQSLILKNHADC
                     GLRNVWARILKDYIGVFNNTIASLQFVLDDYLENAWQNVYQEGPVALERVAREFPGEQ
                     GILANETHKINMQEKLLAMDDEIAEAAIFAQQISADDHLAVAQCNALTSWITRVLRFR
                     TTGTAEKGIKFQFELSDRSTGTLVDFKTFLTTCMLSFDKEGGNPPSTHLMSAERIPAI
                     DGTTVYPLRYGQPFVDTIWQLLNQDARGSSMAVLRVLPKPLNEPNYFFQSTWLVTHCQ
                     TQDTYAQRRIADELYPPRIVQHWLSSNGTPVINPQLLELLNGEYAKHETSQKFYADIN
                     LRPNLWQEIEELVSPDTWKEMVERVYTSDREQQQATFGEQARLQLMAVKAVVVCSRNL
                     LEEIV"
     gene            7327..9807
                     /locus_tag="J1Z28_002226"
     CDS             7327..9807
                     /locus_tag="J1Z28_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013367830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBA7717057.1"
                     /translation="MEEEWSRLQQALTTYFSQGVTFKDGIENQFLNRLIQIITDASAG
                     HGDIFAGYADALRAAHASGISEPWLPLPPELTDAPAPEWGMTKLPAVQQIQLAEESDL
                     ALTRATYQRKIRRNLLRPETDPALRRLLPEEPWLTHYQGFGQQAAIRTLLTSDNNCTL
                     LVNLPTSCGKSLLTQLQALSAPTNTLTLVIVPTVALAIEQADDMQKLLRGADQDHGGS
                     YVWIGGQDQPSRQAIKERIKAGTQRVLFCSPESVRSSLVPVLFELSRLNMIGAIVIDE
                     AHLIDQWGAEFRPDFQLIAPMIRSLKAMGKCRFRTLLLSATYSKSTRDILVEQFHDEN
                     SELIEVSANFLRPEPEYFVHHEANENAHRQRVEQLLMTLPRPLILYCTTRTDAEMWSA
                     RLNELGYWRIGIFHGQIDTNNRKKLISKWKNDQLDIMVATSAFGVGMNKKEIRSIIHA
                     AIPENIDRYYQDCGRAGRDGRSCQVHLVWHKAQMKTAKSLAVETLIGVEKGFARWKAM
                     FDDRKKSPIADYMISLNTKPVHIDHQGDKNEAWNRRTLLLMQRAGLIKLVFAEPKIPD
                     DLRDNLDDNNTQLIEWFESYYNHVHIRHCDDAHYSEAHWNTYVEKIRQSELNNRRAAF
                     TVMETWLEDPGKPLCKILQKFYEYQSHPPELTCGGCPGCRQKGKRAFSPTVGTDVQIL
                     RSEPKKKALFVGAEKKVYYNENMKLHSLLMELRVTLRSLITSGEYVFIRADRAVFLQL
                     ENNLDSLSHFWSAQKMTAPASDGPEIVIIPNTANAFPDLPMTRFERIIIAPEHLADPQ
                     FPHRTWCASAANALSLDSFIRQLQHVNN"
     gene            9794..10669
                     /locus_tag="J1Z28_002227"
     CDS             9794..10669
                     /locus_tag="J1Z28_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717058.1"
                     /translation="MSIINNANPGSGLILLPLIERVLQSAQEPLSQDTLLARYRPDNL
                     PANDNAWRKLKENLSFWCNLGLWPMLDGKMLPLEAGVRPLAHRLLICTIDACREKGVA
                     SGNDCEPLWRVLSCLLSLQQHSVGGREPLSPTIITNKVHKWLPGETINSNTEKLVREF
                     GRFLGFLELMPDGNYVTDPTRAIQYFLPEIFDDTHTLPAKIFMANMAEVMPMMDGGQY
                     RNSVHDVMQQEKDWIAPEGDNICTSTSVALQRLEISRRIILSIHSDDKDALSLHLPGG
                     KIRRVSQITLREDAE"
     gene            10666..13872
                     /locus_tag="J1Z28_002228"
     CDS             10666..13872
                     /locus_tag="J1Z28_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717059.1"
                     /translation="MSIMEYWPSRKNIVQCIRNEAEELEDHVLLAVHEPMLLTRRDVN
                     ENHTILRDEDLFQQLLHTERAIPLIGRSGMGKSHLVRWLDCRFNMYLAENNKNEQWEI
                     VRIPKNASLRQVLLRILKNLTGEFFDNARMRVNEVTEQYPAKDLADLLLTMMAHQLQD
                     MQKAVKEEGQTLLAQGKDIPADRLAYMEAIIEEVENGICDLITDPNFKQNLLNPGHCI
                     FKLASRMSSGLKEGEEDHDKYELLPEDLYFIFEPDDLSARARRYLNHSQLHESDQVEA
                     RARAARVLNEAMHTSLKSLFKRLFTFNGGSFQELFQDIRKEFLRLNRRLVILVEDMAA
                     ISAIEDVLIDSLIEEAAPGGVQVLCPVHSVIAVTDNYPGYKRRQETLITRAGYEWVIE
                     NVDKDNNEDRIVSFCGRYLNAARFGSKELKKRPEQGKSLWPPVWHSDEIPDALEDFGY
                     SANQKFPLFPYNRSAILALADKHCRDSSGELIFNPRTIINRILLDILRDCRIEAEKTQ
                     FPPANFAGIHAVPEIEDKISQLVVSRVGSSVTLAAIWGYGIDSFDELRVKLPSSVAST
                     FGLDELAAQLNGKGPTMKSVEPVTKNAHETTGKTGSTPAGKKIDNPEFEPKEEAKYTA
                     KSELPFIRAVRAWVNDNVILPQEVARDLRTALNACYEIHAQPELFGMSSRPPLKEGRF
                     TLIFLPKAASNPSSIITNFYSEQDLTDPSRRVDVYNAAVALLRIQHYADLKQGFNYPE
                     AQEDYTRYQNFIQWWVPQATESAINHFRARNLRDALKKHLQKALLLGLVTGKEEPAEI
                     VNTLCLTQKGVGRTEGKSTGRTLTTAPSEICFRGIEATLHPHASARVAEARALALVGW
                     DDARKAWLEQVAITEQGSGLGSSRALDADVIYSALKTVLKQPLGAEIERAALSVKQEL
                     ASTLKNVQLFANIKDSDDFLRQLNDWQTFIETVRAEHYPTGSAQYKPSSWLLGEIEAL
                     RTNSEESLATLNALLSIEKQKDSAKLWLEICQLDGDYVSRISSILAQWQLVQPKIAFT
                     LQQMNAKGDIQQLTLALNKVNDRLKTLASDIIASGVDQ"
     gene            13869..14717
                     /locus_tag="J1Z28_002229"
     CDS             13869..14717
                     /locus_tag="J1Z28_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717060.1"
                     /translation="MTTLRQQLAEMQKELDLSFQAGKASQIIDKCNLVCSKTNTIADK
                     FVLLKQQYSAQQQLPRGIAVTWNDEQRSELIAAANSVKSLMLQWRRWLKKQSVDNSGE
                     EENLADQEESPQESLGETLTASEEPTAYDIIQNDSLTHCVNQLGALHKDLSKELKDAW
                     NTWLEELHHQVHVDDKTLNFQKNTNKFDDIASDYERRRLDFENLTQSQPVSDEKIHQI
                     KALAEKLYSLRGMMKTDWPDVVQAFFNRINGPQNLRPTLSALTPEVLEWLTVEEMLDE
                     FVITRK"
     gene            14721..19157
                     /locus_tag="J1Z28_002230"
     CDS             14721..19157
                     /locus_tag="J1Z28_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBA7717061.1"
                     /translation="MSTADLSLLHQALDLLEEREAELLVWGDTDGAFTHADIISLFTR
                     HLPYEDAESLLAELQKAAMLFSVPTSKGGTRYRTRMAEAVCLFRHQRQWFRNQPLQQA
                     RTLVADYRFVRRPRNYPKRELPAQTVLSKWVDVPWMNEIKRQALSRLIGDFFIAGFQE
                     RSTERILRAWHFHSHHARVKEATGTIVCAGTGSGKTLAFYLPALTSLLNDIQRDNAQR
                     VRTLALYPRKELLKDQFMETWSKCRELDNQALVLTGRKIRIGSFFGDTPFNHQYAMKD
                     KDKDMPFDLLRCTTPKCSGQMHWKAEDIKAKKEILRCSHCNHSVDSDEVILTRVSLMK
                     NPPDILFTTTEMLNQHLGNNQTNHLFGVGIDVTPPPVVLLDEVHTYVGNTGAQTAYLL
                     RRWMQLARSHPHFVGLSATLSDAERFFADLVGAHKKHVALIEPKFHEMEDEGAEYLLA
                     LRGDPVSQTALLSTTIQTSMLARRILDTKRKKSCGTWGQKTFVFTDDLDINNRLYHQL
                     CDAEGWRSRGQNLYVAKPPLASQRLETPKNSHKLTLAGQNWRIALDIGHSLDENDRAI
                     VARTSSQDVGVDPRAEVVIATASLEVGFNDPSVGMVIQHKAPRNVASYLQRKGRAGRS
                     RTMRPWMVAVLSEFGRDRVVYQRYEELINPEIKGQLLPMGNIHIQKMQAAMATLDWLS
                     MKIPGSNIWSLLNKPQTKRESLDHLDRMLHLITAVIEQHAWQLELEKYLFYALRITDE
                     QLQRVLWSPPRSIMMELLPTLKRQLTTQWSRMGQPEADRSTGRSPLPAFIPSALFSEL
                     NLPELEIHLERKFEKREHLPFIQGLKEFAPGRISKRYAVYSDNEADWLIPNNFVPAMN
                     CVSDVDFEIEQAFGNTWQPECVIECEGQPPIRVIRPMQVLTRALRFDQNLTEKSNSQL
                     YWHTRFNPDDQAVPLPIPTGPWAEILRSITFFTHQNMTPLDVTRYATHATASLRNKTK
                     QQAHVNFRWVEHGEPVGVGVRQWVDGARLRFTLPPNLLQRLRHDPAIQRALRTLYFQH
                     RIKREPRFEYDTFTADWIYECYLAAVTRELVTQSSLADSIAELKTPDGRRRLADIADT
                     LFQADNIFENCEDGEDDVESQELQQNLRNLFKETDILDMIDRHSAILTQDLSDDVDFQ
                     DWLQELLTTTLAGAVKQTSHLLLPDVDERGLLVDTELNDEVLDVWLTESEPGGCGIIT
                     RMEDVFHQDPVSVLNLFMRSFAVSDYEQIDYNLFEMLSRLSSSSELQEALNAIRQASS
                     HLQRRQANAHLRALLKAQGFALSHSFMSVLHTRVLRPGSQASHDAQMLAYLNAWRELE
                     DKAGYEIALNIFAHTQATQELPDASVIKVFERFCKIQGMLWQRGNAIRRSVLSYYNPF
                     KSGNNLTERLLLSSLFQQTACSISISESDWLAQLHHAITQHGFAELHIPREARHRIVE
                     VISLVQVTPIEYFGLHLYPRESAVDYQDGNLVLRFELAEALL"
     gene            19169..19699
                     /locus_tag="J1Z28_002231"
     CDS             19169..19699
                     /locus_tag="J1Z28_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD-like domain protein"
                     /protein_id="HBA7717062.1"
                     /translation="MEEQRQIFLHGPLGQRHLREILSAQFSGLILYPELIWLISPWMS
                     DFDVIDNRGGQWSFLDPSWGARMISFQELLATAVNNGCPLRIVTRKDTHSVVFIERLL
                     MRLSPDHDMQYLYHENLHAKGLLTKHFFLKGSMNYTWSGANVNDEHLLFSTNNTVISD
                     ALIEFSGQYTFGDTHD"
     gene            19692..21875
                     /locus_tag="J1Z28_002232"
     CDS             19692..21875
                     /locus_tag="J1Z28_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717063.1"
                     /translation="MTNVPQWLRDFFGNGNLLKLDRLLDNVENAYPADLKAVLLPLYE
                     SATDAQWPLILPWCDAHRWVFFAAADTARTTQELSNVLNARLGSADVIADRKVTFVPA
                     QGATSLSETALLTHCPAGFIRIELLPTKLKDKPAKERVFAALKDVIVLFRDRPSIVRT
                     VKRPFGRILSDFILANSQKDEATSDALLQELKNNGTLSRRNLMLLELQQAGKLEKWDT
                     LLNHDSLADLVRGRIPTTLMRMLLKAYQQRYFTPDSHGYPQASPADLRPQCLALNPLF
                     TQIPFLSQDETDFAAWKTWATGVMLIGEVDLLNALPERLKTDWLNGLHTWASRPFNLV
                     SPSATTATTSVPDTLQQLAAYLQTSLTATQEEIIGYAQTLHTLDQQLIEEAMAVPLLK
                     TLIEEIRHLTNPQIVGWDICFSRLCQSEVDSNSLVQLVALESENWPADSFHEATLLQL
                     LSSQIPPDAFPLLRNVMPAFIEWLERHHFSLSSRTWLKWLDVLAMEQSVSQADIKLAT
                     MATERFLQGSVSQEAYQQFGAMLELIVERASSFRNLSVLGELIELFLDSPVQDQATLT
                     SLWLSVQSFVSGIWTRLDPTARTVMRNLAADVLGEGAESVFPVEQDSCSADAEDELPD
                     LSGARVAIYSLTEGAARRAKRMLETLFPGIGVEISQAHTATDKLINQAKQADYFIFSA
                     GSATHQAFYAVSAQRRDLIYPTGKGAGSMLNAFITHVQQVSTVVV"
     gene            complement(21899..23026)
                     /locus_tag="J1Z28_002233"
     CDS             complement(21899..23026)
                     /locus_tag="J1Z28_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016538301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HBA7717064.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEEN
                     LVGYQRPEVRNHIREIQRYIESKNPMIPNPVIIAFDKRVRFEPLTDNGDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEIDSFMMPVSAFIANDAEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLTQRLNFGEGPLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPSTGLGDEVKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSSGAWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDYLVTSYIKAANANI"
     gene            complement(23023..>23949)
                     /locus_tag="J1Z28_002234"
     CDS             complement(23023..>23949)
                     /locus_tag="J1Z28_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HBA7717065.1"
                     /translation="YRVEEVIEFYETSRFNYGVSLDHIVFGYERPGEVFSGEVLTECR
                     RRQDITLTLAQEFLNKSQKSCFTPFGVAHGWNKNSYRQSVEALLAMGYKNITMGGMVP
                     LKTTQILETLEEIKPLLKSDTRVHLLGIARPESFADFIKFGVTSIDSTTPLQQAFKDR
                     KNNYHTPDGPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMNALFEYDKGA
                     LPLEKTLEAVLAYERLHSGEKEANKIRADYERTLGERPWKQCQCNICQAIGINVIIFR
                     GAERNRRRGFHNIQVLYNRLQHTLSLRSEELS"
BASE COUNT         6331 a         5949 c         5915 g         5754 t
ORIGIN      
        1 cgtaatacgg gatagttacg atacgcagcg atagcgctaa gttttagtta aaatccccca
       61 tcagcgggat atggggatct ctgggaggtt caggttttag catcgcgact ttaatacgac
      121 agacgctggt gacgatgatg ccaagtagtg actcttccga ataaggttcc cactgaccaa
      181 ttggcgcgcg attcgcaata acatctccct tcgcccagta ttgggaaaag tagatacatt
      241 caaactgtgt acgctgtttc gtctcacagt tcacgacatt gctggccgat gagctgacca
      301 ttgggcctat ccgggctgtc ccgatggata ttacgccgaa gtatgtggga attgccagcg
      361 gattgatgaa cgccggttcc gctgtcgccg acattatttc acctatcgcc tttggcatta
      421 ttatcgataa aacaggcaac tggagtttac cattctacgg ttccgtcgca ttgcttgtta
      481 ttggtatatt cctgacgttc ttcatgcgtc cagataagtc attgtaaaag tcgaattcag
      541 ggctgaagtg gtctactgaa tttggccatc tgaacagggt gatatgctca ccttagaaca
      601 acacaggtgc accaatgaaa agaagaaatt tcagtcctga attcaaacgt gaatccgctc
      661 agttggttgt cgatcaaaat tacactgttt ctgacgctgc taaggctatg gatgttggtc
      721 tttccacgat gacggaatgg ttcaaacaac tgcgtgatga gcgtcagggc aaacgccaaa
      781 agccttcctg ataacaccgg aacaaatcga actacgtgag ctaaagaaaa agctacaacg
      841 aattgaaatg gaaacgaaat attaaaaaac tttccatgtg tgaaaccccg tcccgagtct
      901 cagaaactcc ccggtaaagc tggcagtcta atatagcgaa ggaagcagtc gtcggccttt
      961 tggtggtatg acccttaaaa aaacatggtt caggggctgt ggcggatccg acggtggtag
     1021 cttgatcccg ttcaggaagg tgatttctcc gtcgtctcct ccttcgttca ttaaaaatga
     1081 gcaaggagat ttatatgaaa aaatcctccc gaatgtctat tgtccattct catgctgcgg
     1141 gcgttgatat tggggccaaa tttcacgttg tggccgtacc gcctgatgct gacgccgagc
     1201 caataaggac ttttcaaagt ttcaccggcg accttcatcg tatgtctgac tggctcaaaa
     1261 cctgtcatat tacaaccgtc gccatggagt ccaccggcgt ttactggttg cctgcttttg
     1321 agatccttga agcagcgggt tttaatatga tactcgttaa tgtcagtgcc gcaaaaaatg
     1381 ttcccggtcg caagactgat gtcaacgacg cacagtggtt gcagagacga catgcatttg
     1441 gtcttctccg ggccagtttc agacctgtac acgacatttc agtcctcagg tcttatctga
     1501 ggcaacgaga acgcttgact gaataccggg ctgcgcatat tcagcatatg cagaaagcgc
     1561 tgatgcagat gaatgtgcaa ttgcatcatg ccgtatcgga tatcactggc gtcaccggtc
     1621 tctcgatcgt cagagctatt gtcagcggtg agcccgatcc ttcagtattg atccagtacc
     1681 gtgatgtgag atgtaagaag acgccagaag tacttcagca ggcactgaca gggaactggc
     1741 aacctgagca tctttttgca cttgagcagt cggtggcatt tttctatttt taccaggaaa
     1801 agatccgtga acgtgacgat caaatcgaaa ttcattaata caactcagca ccgggactga
     1861 aaatcctgaa ggagtattac cctgcgccag acatcgtaca aagcaaccca atcagctttc
     1921 cttgatgtca gaccgttact ctggaaaatt accacggcag atctgacaca aatacatggg
     1981 gttgggcctt atctggcact aaaattcgtt gccgaacgcg gtactgacat gaatcgctgg
     2041 cctgatgcga gccattttac ctcgtggcta ttttgtccac gggaaataag atctccggtg
     2101 gaaaagtgtt gtcctcgaaa acgagacgtt catccagttg aattgctgct gcactcaggc
     2161 tggcagccac aacgattggt cggagcgata ctgcgttggg tgctttttat cgtcgattat
     2221 catcccgtat aggtaaagca aaagcagtca ctgcaacggc aagaaaactg gcaacactgt
     2281 tctacaatgc cttgaaatat ggtaaaaaat atgtcgacaa ttgtgctgat tactatgaag
     2341 agcgttaccg tagttgcgta ctcaatggac ttaaacgaag agtaaaatca ctgggttatt
     2401 ctctccagca agaccctgaa ttatgagagt gaaccaggag tttctcagga aggctaccgc
     2461 gctcttgatg tcagattcct gaacagtctc aataatcggg aaactcagag cgcattatcc
     2521 tgtgttcaca ctctgtcatg tgttcgcagt tcatcgctgc agctacagat attggaaaac
     2581 ccgtcctgaa aaaacagacg gcagatgggc taggtaattg acttgcatgg cggagctagg
     2641 agcattgcca caatggcaac ccggagagat taccagatgg gacgctggct tgctggcagg
     2701 ctcatgaaaa aacaggggct ggtcaactgt cagccgccgc cccaccggtc atgaacatgt
     2761 cgctattcct aactatcttg aaccgcactt cgctgtgaca cagctaaatc aagtgtgatg
     2821 acgtgacgta tatctggata ggtaaacgat ggggatacct cgcagtagta cttgctctgt
     2881 tcgcaataaa gcccggggat gggcaatgtc gttctcaccg gacagcagac tgactataaa
     2941 agcgctgaag atggccgagg aaacgcgagg taaaccagag ggagttatgt tccttagcaa
     3001 caggagagcc attacacaag tagacagttc aggcaggtga tgtggcgtta tcggatcaag
     3061 gagagtatga gtcgtcgggg aaactgctgg gatattagcc taatggtacg tttcttcaga
     3121 agtctgaaga acgagtgggc accagtgact ggttacttta gccttaacga aggtgtccat
     3181 acaataacag agtatataat tgaatattaa gtgcactccg gcctcatgaa tataacggtg
     3241 gattacctcc gaacgaatcg gaaaatagat actggaaaaa atctgaaatt gtgaccagtt
     3301 tttgttgacc actacacaat acagtagtgt taatcactaa gtcataaatt ctaggaagtc
     3361 ttgcttcttg ttgttactat ataacaaaaa acgaggtcgc tatgaaaaca acattgtaca
     3421 aagatttcac ctttgaagcc gcacaccgac tcccaaacgt gcctgatggg cataaatgtg
     3481 ggcgtttgca cggacactct tttgtggtac gtctggaaat taccggagag gtcgataaaa
     3541 acaccggttg gataatcgat tttgccgagt tgaaggcgat tttcaaaccc acgctagacc
     3601 gactggatca ctactacctc aacgatattc ctggtctgga aaacccgacc agcgaagtac
     3661 tggcagaatg gatttggaag cagattaaat cacagttgcc acacctgacc gctgtaatgg
     3721 tgaaagaaac ctgcacagcg ggttgcattt atcgcggtga atgatatccg acccgatgac
     3781 tggtttggat aaaaccagtc acattcctta gtcgatggca acaaacaacc ttccatttta
     3841 taacttgcat cacaattctc ggcagtttat gttttcctta gtggcgttga tatgaatatt
     3901 taatattcga tggtgatcat tttattatta cttaacaatt atttttgaat tcatccagcc
     3961 tacattagta gattgaaaaa tactcattgt aacagataaa attgatatcg ctgaaatctc
     4021 ccctacacaa gccaacatct cattgatctt tttctacttt cccccttgaa tatgagaatt
     4081 atctcagtat cctgatcata atactcaaat caatgcactt ataagcatgt cgctcaggca
     4141 tagcaaagta acattttcgg tctgtctttg gttgtttttg agcaaatgtg attataagaa
     4201 acaattcaag gatgtgagga tgaacgtatt acccatcggg tcactggtta tctctgagca
     4261 atttgaaggc atcggtaaag ttgtcaccgt cgatagtgat acaaataacg ccacggtcgc
     4321 tttttttgag tcaccggctc aaccttatgc acgacaaatg aaagttcctt tagaacaact
     4381 caccctgaca atcccgcacg aagagacggt aatttattgc attgagccac atagccaacg
     4441 atggactaga gcccggtttg gcggatcgcg ccccaaaggg gattttctcg tcatctttcg
     4501 cgaagatgaa actacgacgc tgccaatcga cgaaattttt gttctgaaca aagcgccgga
     4561 taccccgata aaccctgcag atttccttgc cctccaagct aatgacgcac cgttcttttt
     4621 cccttaccgc caagccttta ttgaaaccta catccagcaa cgcgctgcat gccgcgcgat
     4681 ggcatccatt tctagtagtg ccgttgaact cgaaccccac caattggcgg tagtacgccg
     4741 tgtacttcag gataaaaacc ccaagtatat tctggccgat gaggttgggc tggggaaaac
     4801 catcgaagca ggaatggtca tccgtgaaca cgcgttagaa gcaactggcc acgtcagcat
     4861 gcttattgcg gttccggcac cactagttag ccagtggcgg gaagaactgg ctgaacgttt
     4921 tcaactgaaa caacttatta ttgatgcttc gacggccctc gcaggtttgc gacaaaatga
     4981 ggccacggaa ggcatcgtga tttgcagtca ttgcgatggc tgtaccctga ttgaacgggg
     5041 attcacccct tcacttatcg cagtagatga ggtccaccag attgcctctt ggccgtggtc
     5101 aggagataaa gatgaacgtt atgacttcaa cctgatcgca gaggggtgcc ggaaagccca
     5161 ctacgttctg ttactcacgg ggacccctct gcatgggcat gaaaggaact tcttgtcgat
     5221 gctgcactgt attaatccgg aagcctacca agtggatgag acccatctgc aagactttac
     5281 cgaactagtc aaaaatcgcg aaaaccttgg cgggatcttc tcggggttag taccttccgt
     5341 ctcaaacgtc agcttacgtc gtaatctgga aacactgcag cagcagttcc cagaagatac
     5401 tgttctgatg acgctctgcc agcaactcat tccgcttatt gggacgttct cccccaatac
     5461 gccggagcgt gaagaaggga tccgggatat tcgccggcac ttgggggaaa actatcgcat
     5521 tcatcatcga ctgttgcgta atcgccgtca ggtttcactc agcttcgatg aaacgaaaaa
     5581 cctcaatcta aaccttctgt ttcctgggct aaatggagca gaggagtgcc gctggtattg
     5641 cgatgggtta gcattagacg aactgattga tgagtatcgt tcattatctt tactgccgca
     5701 gaatacgcag tgggcaatga gtgaagacac gtacctcttc tgggttgacg atctcctatc
     5761 cagcccactc tgtgttatgc aacgcgctaa agtacatttg ggtgaagcgg aacgcactca
     5821 ggaagaacga gatctgttag agcacattgt ttcaactgca aaaaatgaac tacaggccat
     5881 tgattacaag cttgccatca cactgaatga gtggttgcaa aagaatccga caggaaaagc
     5941 aatcgtcttc tgcgatcgtc cggatcttgc ccaacacttg gcaataaagc tcgaactact
     6001 gctggagtac ccccaggaac tctatcagcc gggtcatccc ttgatgtacc tcaaacccgg
     6061 cagccctatt cggatcctaa tttgcgataa aagcggcgaa gatggtctga atctccatgg
     6121 ggggctgcga ctagccgttc actacggttt accgcgctct tgtagtcgta ttgaacagcg
     6181 ccttggtcgt ctgaacaggt acagtgcaaa cctgaaaggc gtaaaacctg tccagagtct
     6241 gatcctgaaa aatcacgccg actgtggatt acgcaacgtg tgggcccgca ttctgaaaga
     6301 ctatattggc gtatttaata acacaatcgc cagcctccag ttcgtcctcg acgattatct
     6361 agaaaatgcg tggcaaaatg tctaccaaga aggcccggtg gcccttgaac gtgttgccag
     6421 agaattccct ggcgaacagg gcattctggc caatgaaacg cataaaatta acatgcagga
     6481 aaagctgctg gcgatggatg atgagattgc agaggcggcg atttttgcgc aacagatcag
     6541 cgctgacgat catcttgcag tagcgcagtg taatgcactg acaagttgga tcacgcgtgt
     6601 gctacgtttt cgcaccaccg gcactgcaga aaaaggtatc aagtttcagt tcgaactctc
     6661 tgatcgcagc acagggacgc tggtcgattt taaaaccttt ctcaccacct gcatgctcag
     6721 cttcgataaa gaagggggca acccaccgtc aacgcatctg atgagcgctg aacgcattcc
     6781 ggcgatcgac ggaacaaccg tttacccgct gcgttatggg cagccatttg tcgatacgat
     6841 ctggcaactg ttaaaccaag atgcccgagg cagttccatg gcggtactgc gagtgctccc
     6901 caagccactg aatgagccca attacttctt tcagtcaact tggcttgtca cccattgcca
     6961 gacgcaggat acctacgcac aacgccgtat tgcagacgag ttgtacccgc cgcgaattgt
     7021 gcaacactgg ctgtcctcta atggtacgcc ggtgataaat ccgcagttgc tggaactgct
     7081 caatggcgag tacgccaaac atgaaacatc gcagaaattc tatgcagata taaatctgcg
     7141 ccctaatctt tggcaagaaa tcgaagaact tgtctcaccg gacacgtgga aagagatggt
     7201 ggaacgagtc tataccagtg accgagaaca acaacaggca accttcggcg aacaagccag
     7261 acttcagtta atggccgtga aagccgtggt ggtgtgcagc agaaatctat tagaggaaat
     7321 cgtctgatgg aagaggaatg gtcacgcctg cagcaggcgc taaccaccta ttttagtcaa
     7381 ggggtcacct ttaaagatgg catcgaaaat cagtttctga acagactgat tcagatcatc
     7441 accgatgcct cggcaggtca tggtgacatt tttgccggct acgccgatgc gttacgtgcc
     7501 gctcacgcga gtggtattag cgagccgtgg ctacccctgc cgccagaact gaccgatgct
     7561 ccggcacctg agtggggcat gacaaagttg cctgccgtgc aacaaattca actggctgaa
     7621 gagtcggacc tggcgctgac cagagccacc tatcagcgta agatacgcag gaacttgctg
     7681 cgccccgaga ctgatccggc actgaggcgc ctgttacccg aagagccatg gctgactcac
     7741 tatcagggat tcggtcaaca ggccgccatc cggacgctgc taacgtctga taataactgt
     7801 acattgctgg ttaatctgcc caccagttgc ggtaaaagtc tgctcaccca actccaggcg
     7861 ctcagcgcac cgacaaacac cctcacgctg gtgatcgtcc ccactgtcgc cctcgcgatt
     7921 gagcaggctg acgatatgca aaaactgctt cgcggtgctg accaagatca tggtggaagt
     7981 tatgtttgga tcggaggcca agatcagccc tcgcgtcagg caattaagga aagaatcaaa
     8041 gcgggaacac agcgtgttct cttctgttcc ccagaatccg ttcgctcttc tttggttccg
     8101 gtgctgtttg agctttccag actgaatatg attggcgcga ttgtcataga cgaagcgcat
     8161 ttgatcgatc aatggggcgc agagttccgc cctgactttc agctaattgc ccctatgatt
     8221 cgctcactga aagccatggg caaatgccgt tttcgtacgc tgctgctttc ggcgacttac
     8281 agcaaaagta cccgcgacat tctggtcgag cagttccatg atgaaaacag tgaactgatt
     8341 gaagtatcag ctaatttcct acgcccagag ccggaatatt ttgtgcatca tgaggcaaat
     8401 gaaaacgcgc atcgtcagcg ggtcgaacaa cttctgatga ccttaccccg cccgctgatt
     8461 ttgtattgca ctacccgcac agatgcggaa atgtggtctg cccgtctgaa cgaactgggc
     8521 tactggcgaa tcggcatatt ccacggtcaa attgacacaa acaatcgcaa gaaattgatt
     8581 tcaaaatgga aaaatgacca actcgatatc atggtcgcta cctctgcctt tggggtaggg
     8641 atgaataaaa aggaaatccg cagcatcatt catgctgcta ttccggaaaa tatcgaccgc
     8701 tattaccagg actgtggacg tgctgggcgt gacggcagat cctgccaggt gcatctggta
     8761 tggcataagg cgcaaatgaa aacggcaaaa tcgctggccg ttgagacgct gattggcgta
     8821 gaaaaaggtt ttgcccgctg gaaagcaatg tttgacgatc gtaagaaaag cccgatcgct
     8881 gactacatga tttcactgaa caccaagccc gttcatatcg atcaccaagg cgataaaaac
     8941 gaagcttgga atcgtcggac gttgctgtta atgcaaagag ccggactcat caaactggtc
     9001 tttgctgaac cgaaaattcc tgacgacctg cgagataatc tggatgacaa caacacgcaa
     9061 ctgattgaat ggttcgaaag ctactataac cacgtacata tccgtcactg tgacgatgcg
     9121 cattattcag aagcgcactg gaacacctat gtggagaaga tccgccagtc cgagttgaat
     9181 aaccgccggg cagcctttac ggtaatggaa acgtggttgg aggatcctgg caaaccactg
     9241 tgtaaaattc tgcagaaatt ctatgaatat cagagccatc ctcccgagtt aacctgtggt
     9301 ggttgtccgg gatgtcgcca gaaagggaaa agggcgtttt cgcccaccgt gggcacagat
     9361 gtacaaattt tacgttccga gccgaagaaa aaagcactct tcgtaggtgc ggaaaagaaa
     9421 gtttattaca acgaaaatat gaagttacat tcgttgttga tggaacttcg tgtgacgttg
     9481 cgatcactga tcacctctgg cgaatacgtg tttattcgtg cagacagagc agtgttcctg
     9541 caactagaga acaacctaga ttcgctttcg catttctgga gcgcacagaa aatgacagca
     9601 cctgcatcgg atggaccaga gatcgtgatc atccctaaca cggctaatgc gttcccagat
     9661 ctacccatga cgcgctttga gcgaattatt attgcacctg agcatctcgc cgatcctcag
     9721 ttcccacatc gaacatggtg cgcaagtgcc gctaatgcgc tgtcgctgga ctcttttatc
     9781 aggcaactac aacatgtcaa taattaacaa cgcaaaccca ggaagcggac tgatactgct
     9841 tcctttgatt gaacgcgttc tgcaaagcgc acaggaaccg ttgtctcagg acacactgct
     9901 cgcccgctat cgtcctgata acctgcccgc taacgataac gcgtggagaa aattaaaaga
     9961 gaacctgagc ttttggtgca atctgggact ctggccgatg ctcgacggaa agatgttacc
    10021 gcttgaagca ggtgtgcgtc cactggcgca tcggttgctt atctgtacga ttgatgcttg
    10081 tcgtgaaaaa ggcgttgcca gtgggaatga ttgcgaaccg ttgtggcggg tactgagttg
    10141 cttgttgagc cttcagcaac actcggtcgg gggccgggaa cctctttccc ctacgatcat
    10201 caccaacaaa gtgcataaat ggttacctgg cgaaaccatc aatagcaaca ccgaaaaact
    10261 agtgagagaa tttggtcgtt ttctcggttt tctcgagtta atgccggacg gaaattatgt
    10321 caccgaccct acccgggcca tccaatattt tctgccggag atctttgacg atacgcatac
    10381 ccttcccgcc aaaatcttta tggcaaacat ggcggaggtc atgccgatga tggacggggg
    10441 ccagtacaga aactccgttc atgacgtgat gcagcaggaa aaagactgga tcgcaccgga
    10501 aggtgacaat atctgcacca gcacatctgt tgcgctgcaa cgacttgaaa tcagtagacg
    10561 cattatcctc tccatccaca gtgatgataa agacgcctta tcacttcact tacctggcgg
    10621 taaaattcgc cgggttagcc aaatcacgct aagagaggat gcagaatgag cataatggaa
    10681 tattggccat ccaggaaaaa tatcgtccaa tgcattcgca atgaagcaga agaattagag
    10741 gatcatgtgc ttctggccgt acatgaaccg atgctactga cccgacgcga cgtcaatgaa
    10801 aatcatacaa tattgcgtga tgaagacctg ttccagcagt tacttcatac ggagcgcgcg
    10861 atcccactga ttggccgctc ggggatgggg aaatcacatc tggtgcgctg gttagactgc
    10921 cgattcaaca tgtacctagc ggaaaacaac aagaatgagc aatgggaaat tgtccggata
    10981 cccaaaaacg ccagtctacg gcaggtgctt ttacgcatcc taaaaaattt gacgggggaa
    11041 tttttcgaca acgcacgcat gcgcgttaat gaagtgacag aacagtaccc agcgaaagat
    11101 ctggcagacc tactgttgac catgatggcc catcagttgc aggacatgca aaaagcggtc
    11161 aaggaagaag gtcaaacatt gctggcgcaa ggtaaagaca ttcctgcaga ccgactcgcc
    11221 tatatggaag ctatcattga agaggtcgaa aacggtatct gtgatctgat caccgaccct
    11281 aacttcaaac agaatcttct gaatccaggc cactgtattt tcaaacttgc cagtcggatg
    11341 agtagtggtc tgaaagaagg agaagaagat catgataaat atgaacttct tccggaagat
    11401 ctctatttta tctttgaacc tgacgatctg tccgcgcgtg cacgccgcta tctgaaccat
    11461 tctcaactgc atgagtcaga ccaagtggag gcgcgtgccc gagcagcgcg cgtgctcaat
    11521 gaagccatgc atacctcgct gaaatcactt ttcaagcgcc tattcacctt caatggagga
    11581 tcttttcagg agctttttca ggacatccgc aaggagtttc tacgacttaa tcgtcgtctg
    11641 gtgattctgg tagaagatat ggcggcgatt tccgctattg aagatgttct gatcgacagt
    11701 ctgatcgaag aagcggcacc tggcggcgtg caagttctct gtcctgtcca ctcagtgatt
    11761 gcagtcaccg acaattatcc tggctataaa cgccgccagg aaacgttaat cacacgtgct
    11821 ggctacgagt gggtcattga gaacgtcgat aaagacaata acgaagatcg tattgtctcg
    11881 ttctgtggtc gctatctgaa cgccgcacgc tttggatcaa aggagttaaa aaaacgtcct
    11941 gagcaaggta agtctctgtg gccacctgta tggcacagtg atgagatccc tgatgcacta
    12001 gaggattttg gttattctgc caatcagaaa ttcccgctat tcccgtataa ccgtagtgcc
    12061 attctggcat tggcggataa gcattgccgc gacagcagcg gcgagttgat ttttaacccg
    12121 cgcacaatca ttaaccgcat cctgttagat attctgcgcg attgccgaat agaagcggaa
    12181 aaaacacagt tcccgccagc aaatttcgct ggaattcatg ccgttcccga gatcgaagac
    12241 aagatttcac aactggttgt cagtcgggtt ggcagttcgg tgacacttgc cgccatctgg
    12301 ggatatggca ttgattcttt tgatgaacta cgcgtaaaac tgccaagtag cgttgcgagt
    12361 acctttggcc tcgatgagct agccgcacaa ctgaatggca aaggtcccac gatgaaatcg
    12421 gtggaaccag taacaaagaa cgcacacgaa acgactggca agacaggcag tacgccggcg
    12481 ggtaaaaaga ttgacaatcc ggagttcgaa ccgaaagaag aggcaaaata cactgccaaa
    12541 tccgaactac cctttatcag ggctgtccgt gcatgggtga atgataatgt cattctgccg
    12601 caggaggttg cccgtgattt gcgtacggca ctgaacgctt gttatgaaat tcatgcacag
    12661 ccagagttgt ttggtatgtc ttctcgacca ccgctgaaag aaggtcgttt tacgttgatt
    12721 tttctgccaa aagcggcatc taacccctca agtattatta cgaactttta ctctgagcag
    12781 gacctgaccg atccttcccg acgggtagat gtgtataacg ccgcggtagc cctgttacgt
    12841 atccagcatt acgccgattt aaagcaaggt tttaattatc cagaggcgca ggaagactat
    12901 actcgctatc aaaacttcat ccagtggtgg gtaccgcagg ctacggaaag tgccattaat
    12961 cacttccggg cgcgtaactt acgggatgcg ctgaaaaagc atctgcaaaa agctctgctg
    13021 cttggcctgg tgaccggtaa agaagaaccg gcggaaatcg tcaataccct ctgcctcacc
    13081 cagaaaggcg tcggaagaac tgaaggtaaa agtaccggtc gaacgctgac gaccgctcct
    13141 tccgagatct gtttcagagg gattgaggca acactgcatc cgcacgccag tgccagagtc
    13201 gccgaagcta gggcgctggc gctcgtagga tgggatgatg ccagaaaagc gtggcttgaa
    13261 caggttgcca tcacggagca aggctcaggc ttgggcagca gtcgtgcgct ggatgccgat
    13321 gtaatatatt ctgcgctgaa aacagtgtta aaacagccgc ttggcgcaga aattgaacgt
    13381 gcggcgctct ccgtcaaaca ggaacttgcc agcacgttaa aaaatgttca gctctttgcc
    13441 aacatcaaag atagtgacga tttcctcaga caactgaacg actggcagac gtttattgag
    13501 actgtgcggg ccgagcacta ccccactggc agcgctcagt acaagccatc ttcgtggctg
    13561 ctgggggaaa ttgaagctct cagaaccaac tccgaggaga gtctggcgac attaaatgcg
    13621 ttactttcca ttgaaaaaca gaaagatagt gcgaaattgt ggctggagat ctgtcaactt
    13681 gatggtgact acgtttcaag aatcagcagc atacttgcgc aatggcaact ggtacaaccc
    13741 aagatcgcgt tcacactcca gcagatgaat gccaaagggg atatccagca actgactctt
    13801 gctctgaata aagtgaatga ccgtctaaaa acacttgcga gcgatatcat cgcctctgga
    13861 gttgatcaat gaccacctta cgccaacaac tggccgagat gcaaaaagaa ctcgatctta
    13921 gctttcaggc tggtaaggcc tcacaaatca tcgataaatg taatctggtg tgcagcaaaa
    13981 ctaacaccat tgcagataag tttgtattgc ttaaacagca atattcagcc cagcaacaac
    14041 tcccgcgggg cattgcggtt acctggaatg acgagcagcg tagcgaactg atcgctgccg
    14101 cgaacagcgt caaaagtctc atgttgcagt ggagacgctg gctgaaaaaa cagtcggtag
    14161 acaattctgg cgaggaggag aaccttgcag atcaggaaga gtcaccacag gaatctctgg
    14221 gtgagactct gaccgcctca gaggaaccta ctgcctacga catcatccag aatgattcct
    14281 tgacccattg cgtcaaccaa ctcggcgccc tgcacaagga cctaagcaaa gaactaaaag
    14341 atgcttggaa tacatggctg gaagagttac atcatcaagt tcacgtcgat gacaaaactc
    14401 tgaattttca gaaaaatacg aataaatttg atgatatcgc ctccgactac gagcgtcgac
    14461 gtctggattt cgaaaactta acgcagagcc agccagtcag cgatgaaaag attcaccaaa
    14521 ttaaagcgct agccgagaaa ctgtattccc tgcgcggcat gatgaaaacg gactggcctg
    14581 acgtggttca ggcatttttc aatcgcatta atggcccgca gaacttgcgc ccgacgttaa
    14641 gcgcccttac gcctgaggtg cttgagtggt taacggttga agagatgctg gatgagttcg
    14701 tgatcacaag gaaataattt atgtctacgg ctgatttgtc cctcctgcac caagcgctgg
    14761 atctgctcga agagcgggaa gcagaactgc tggtctgggg ggatacagat ggcgcattta
    14821 cccatgccga tatcatctcg ctctttaccc ggcaccttcc gtacgaagat gccgagtcgt
    14881 tactggcaga actgcagaag gcagccatgc ttttcagcgt cccgaccagc aaagggggaa
    14941 ctcgctaccg cacgcgtatg gcagaagcgg tatgcctgtt ccgccaccag cgtcaatggt
    15001 tccgcaatca accgcttcag caagcccgga cgcttgtcgc cgactaccgt ttcgtccgtc
    15061 gcccgcgcaa ctatccgaag cgtgaacttc cggcgcaaac ggtgctgagc aaatgggtcg
    15121 atgttccgtg gatgaacgaa attaaacgcc aggcgctttc ccgactgatc ggcgactttt
    15181 tcattgccgg ctttcaggaa cgctcaaccg aacgtatttt gcgcgcctgg cactttcata
    15241 gtcatcatgc ccgcgtgaaa gaagctaccg ggaccattgt ctgtgccggt acggggagtg
    15301 gtaaaacact ggcgttctat ctcccggcac tcacctcgct cctcaatgat attcagcgcg
    15361 acaatgcgca acgggtcagg acgctggcac tgtatccacg taaagaactg cttaaagatc
    15421 agttcatgga aacctggagt aaatgtcgtg aacttgataa tcaggcccta gtactgacag
    15481 gacgcaagat ccgtattggt tcttttttcg gtgatacccc gttcaaccat caatacgcaa
    15541 tgaaagataa agacaaggat atgccctttg atttactccg ttgtaccacg ccgaaatgtt
    15601 cggggcaaat gcactggaaa gcagaagata tcaaagcgaa aaaagagatt cttcgttgta
    15661 gccattgtaa tcacagcgta gacagtgacg aagtaatcct gacccgcgtc tcgctgatga
    15721 aaaacccgcc tgacatcctg tttaccacta ccgaaatgct caatcagcac ctagggaata
    15781 accaaacgaa tcacctattt ggcgttggca ttgacgtaac acctccgccg gtagtcctgc
    15841 tggatgaagt acacacctat gtggggaata ccggtgcaca gacggcctat ctactgcgac
    15901 gctggatgca acttgcgcgc agccaccctc attttgttgg gctttcggca acgctgagtg
    15961 acgccgaacg tttcttcgcc gatctggtag gtgcacataa aaaacatgtg gctctgatcg
    16021 aaccgaagtt ccacgaaatg gaagatgaag gggctgaata tctgctggca ttacgcggtg
    16081 atccggtctc gcaaaccgcc ttgctatcta ctactatcca gaccagcatg ctcgctcggc
    16141 gtattctgga tacgaaaagg aaaaaatcat gcgggacatg ggggcaaaaa acctttgtct
    16201 tcaccgacga tctggatatc aataaccgac tttatcatca actgtgtgac gccgaaggct
    16261 ggcggtcgcg tggtcaaaac ttatacgttg caaaaccacc gctggcttct cagcgtctgg
    16321 aaacaccgaa aaacagccat aagctgacac tggcaggaca gaactggcgt atcgcgctgg
    16381 atattggtca ctcacttgac gaaaatgacc gagccatcgt tgcccgcacg tccagtcagg
    16441 acgtcggggt cgatcctaga gcggaagtgg tgattgcaac cgcgtcgctg gaggtgggct
    16501 ttaacgaccc ctccgttggc atggttattc agcataaagc gccacgtaac gttgcctcct
    16561 atttgcagcg caaagggcgt gctggtcgtt caagaaccat gcgtccgtgg atggtcgctg
    16621 tcctatccga atttggccgc gaccgagtcg tttatcaacg ctatgaagag ctcattaatc
    16681 cagagataaa aggacagttg ttgccaatgg gcaacatcca tatccagaaa atgcaggcgg
    16741 caatggcgac acttgactgg ctgagcatga agatccccgg aagtaatatc tggtcattgc
    16801 tgaataaacc gcagacaaag cgagaatctc ttgatcatct tgatcgtatg ctccacctga
    16861 ttactgccgt gattgaacaa catgcgtggc agctggagct ggagaagtat ctgttttacg
    16921 cgttgcgcat cactgacgag caacttcagc gtgtactgtg gtcgccgccc cgttcgatca
    16981 tgatggagtt gctgccgaca ttaaaacgac aactcacgac ccaatggtcg cggatgggcc
    17041 aaccggaggc agatcgttcg acgggacgtt ccccactgcc ggcctttatt ccttcagcgc
    17101 tattcagtga gcttaatctg ccggaactgg agatccatct cgagcggaag ttcgagaagc
    17161 gtgaacattt gccctttatt caggggctga aagagtttgc gccggggcgc atttccaagc
    17221 gctatgccgt ttactccgac aatgaagcag actggcttat cccaaacaac tttgttccgg
    17281 cgatgaattg cgtttcggat gttgattttg aaattgagca ggctttcggc aatacttggc
    17341 agcccgagtg tgtcatcgag tgcgaaggtc agccccctat tagggtcatt cgcccgatgc
    17401 aggtgcttac gcgcgcgctg agatttgatc aaaatctcac cgagaaaagc aactcgcaac
    17461 tgtactggca tacccgtttt aacccagacg atcaggccgt tccgctcccc attcctaccg
    17521 gcccgtgggc ggagatcctc cgctccatta cgttcttcac ccaccagaac atgacacctc
    17581 tagatgtcac gcgttacgcc acccacgcaa cagcctcatt acgcaataaa accaagcaac
    17641 aggcgcatgt gaacttcaga tgggtcgagc atggcgagcc tgtcggggtt ggcgtgcggc
    17701 agtgggtgga tggcgcacgg ctcagattta cgctgccacc caatctgctg caacgcctcc
    17761 ggcatgaccc ggccattcag cgcgcactgc gaacactcta tttccagcat cgcatcaaac
    17821 gtgaaccacg cttcgaatat gacacgttta ccgctgactg gatctacgag tgctatctgg
    17881 ctgcggttac gcgggaactg gtcacacagt cgtctctggc agattctatc gcggaattaa
    17941 aaactccgga cggccgacgt cgcctcgctg atatcgcgga cacactcttc caggcagata
    18001 atatctttga aaattgcgag gatggtgaag acgacgttga aagtcaggaa ctgcagcaaa
    18061 atttgcgcaa cctgtttaaa gagaccgaca ttctggacat gatagatagg cacagcgcga
    18121 tcctgactca ggatctcagt gatgatgtcg attttcagga ctggctacag gagttactga
    18181 caacgacgct ggccggagcg gtcaaacaaa ccagccacct gttgctgccg gatgtcgatg
    18241 aacgcggtct gctggtggat accgaactaa acgacgaagt actggacgtc tggctgaccg
    18301 aatcagaacc cggcggctgt ggtatcatta cccgtatgga agacgttttt catcaggatc
    18361 cggtctccgt actgaatctg tttatgcgta gttttgccgt cagcgattac gagcagatcg
    18421 actacaacct gttcgagatg ctttcgcggc tctcatcttc cagcgaactt caggaggcct
    18481 tgaatgcgat acgtcaggcc agcagtcatc tgcagcgtcg ccaggctaac gcccacctca
    18541 gagcgctgct aaaggcgcag ggatttgcac ttagtcacag ttttatgtcg gtgctgcaca
    18601 cgcgggtatt acgtccgggc agccaagcgt cacatgatgc gcagatgctt gcatatctca
    18661 acgcatggcg ggaattggaa gataaagccg gatacgaaat cgctctgaat atttttgccc
    18721 atacccaagc gacgcaagaa ctgcctgacg ccagtgtgat taaagttttt gagcgctttt
    18781 gcaaaattca gggaatgctc tggcagcgcg gcaatgccat ccgtcggtcg gttctcagct
    18841 attacaatcc gtttaagtcc ggtaacaacc tgactgaacg actgctgcta agctcactgt
    18901 ttcaacagac ggcgtgtagc atcagcatca gcgagagcga ttggttagcg cagttacatc
    18961 acgccattac ccaacacggc tttgcagaac tgcatatccc gcgcgaggct cgccatcgta
    19021 ttgtggaagt gatctctctg gtgcaggtga ctccaattga gtactttggt ttgcatcttt
    19081 atccccgcga atccgccgtg gattatcagg atggcaatct ggtgttacgt tttgaactgg
    19141 ccgaagcgct actgtaagga acgcagaaat ggaagaacaa cgacaaattt tcctgcatgg
    19201 cccactgggc cagcgacatt tgcgggaaat cctaagcgcg cagtttagtg gcttgatcct
    19261 ctatccggaa ctgatctggc tgatctctcc gtggatgagt gactttgacg tgattgataa
    19321 ccgaggcggt cagtggagtt ttcttgatcc ttcatgggga gctcggatga tcagctttca
    19381 ggaattgctc gccaccgccg ttaacaatgg ttgcccgtta cgtatcgtga cacgtaaaga
    19441 cacgcacagc gtggtcttta tcgagcgact attgatgcgc ttatcgcccg atcacgacat
    19501 gcaataccta tatcacgaga acctgcacgc taagggattg ctgaccaaac atttctttct
    19561 gaaaggctcc atgaattaca cttggagcgg cgcaaacgtg aacgatgagc atctgttgtt
    19621 ttcaaccaat aatacggtga tttcagatgc gttgattgaa tttagcggcc agtacacctt
    19681 tggagacacc catgactaat gttcctcaat ggctgcgtga cttctttggc aacggaaatt
    19741 tactgaagct ggacaggttg ctggacaacg ttgaaaacgc ctaccctgca gacttaaaag
    19801 ccgtattact gccgctttac gagagcgcga ctgatgctca gtggccactc attctgccct
    19861 ggtgtgatgc tcatcgctgg gtattttttg ccgcggcaga tacagctcgc accacgcagg
    19921 aactcagcaa tgtgttgaat gccaggctgg ggtctgcaga tgttattgct gacagaaagg
    19981 tgacctttgt ccccgcacag ggagcgacgt ccctgtctga aacggcgttg ctgacgcact
    20041 gtccagccgg atttatccgg atcgaattat tgcccacaaa gcttaaagat aaaccagcaa
    20101 aagagcgggt tttcgccgcg ctcaaagacg ttatcgtcct atttcgtgac aggccatcaa
    20161 tcgtacgcac ggtcaaacgc cccttcgggc gaatcttgag tgatttcatt ctggcgaata
    20221 gccaaaaaga cgaagccaca agcgacgctt tactacagga actgaagaac aacggtacgc
    20281 tttcgcgacg taaccttatg ctgctcgaac tccagcaggc gggtaagtta gagaagtggg
    20341 acacactgct gaatcacgat tctcttgctg atttggttcg gggacgcatt cctacaaccc
    20401 tgatgcggat gctgctgaaa gcataccagc agcgctattt tacacccgac agccatgggt
    20461 atccgcaagc ctcaccggcg gatcttcgcc cgcaatgtct ggcactaaac cctctgttta
    20521 cgcagatacc ttttttatcc caagatgaaa ctgactttgc ggcgtggaaa acttgggcca
    20581 ctggagtgat gcttattggt gaagtcgacc tgctcaacgc gttgccagaa cgcctaaaaa
    20641 ccgactggtt gaatggattg cacacctggg ctagtcggcc gttcaactta gtgagtccgt
    20701 cagcgacgac agcgacgacg tcggtcccgg atacgctgca acagcttgcg gcatatttac
    20761 aaacctccct gaccgcaacg caagaggaga ttatcggcta cgcacaaacg ctccacacgc
    20821 ttgaccagca gttgatagag gaagcgatgg cggtaccgct actgaaaaca ctcatcgagg
    20881 agatccgcca tctgaccaat ccgcaaatcg tgggctggga tatttgtttt tcccgactat
    20941 gtcagtcaga agtcgacagc aacagccttg tgcaactggt tgcgctggaa agtgaaaact
    21001 ggcccgctga ctcgttccat gaagcgacgc tgctccagtt actatcaagt cagattccac
    21061 cagacgcgtt cccgctcctt cgtaacgtga tgcctgcctt tattgaatgg cttgagcgtc
    21121 atcatttcag tctgagtagc aggacgtggc taaagtggct ggatgttctt gcgatggaac
    21181 aaagcgtcag tcaggcggat atcaagctgg ccacgatggc gacggagcgc ttcctacaag
    21241 gctctgttag ccaggaagca tatcaacagt tcggagccat gttggaactg atcgtcgaac
    21301 gcgccagttc gttccgtaac ctgtcagtgc tgggcgagtt gatcgaactc ttccttgatt
    21361 ctccggtgca ggatcaggcg acgctcacct cgctgtggct cagcgttcag tcttttgtga
    21421 gcggaatatg gacgcgactg gatccgactg ctcgtaccgt gatgcgcaac ttggcggctg
    21481 acgtgctagg ggaaggtgca gagagtgtat ttccggttga gcaggatagc tgttcggcag
    21541 atgcagaaga tgaattgccg gacttatccg gagccagagt ggcgatttat agcttgacag
    21601 aaggtgctgc aagacgcgcg aaacggatgc tggaaacgct gtttcccggg atcggagtgg
    21661 agatcagcca agcacacact gcaaccgata agttgattaa tcaagcaaaa caggctgatt
    21721 acttcatctt ttcggcaggc agcgccactc atcaggcttt ttacgcggtc tcagcgcagc
    21781 gcagagattt aatctacccg acaggtaaag gtgccgggtc aatgctgaac gcgtttatta
    21841 cgcacgttca gcaagtctca acagtagtgg tataaaaata ggggcaaatg cccccttttt
    21901 atatattggc gttcgctgct ttgatataac tggtgaccag ataatccgtc actaatgaga
    21961 tatctttcga caggttctgc agttcattcc atttacgaac aatgggctga ccatcaatat
    22021 ccgtaccgaa gttccacgcg ccactactcc atgcacaaag tggcttgatg cggtttaact
    22081 cttcgacaaa acgagtatag gaaggaatct cagtccagcc aggttcacct ttatagtcct
    22141 gatgaacctg atcgatttca tccatcaaac taccaagagc aagaatacca acgccgtgta
    22201 ataggcggga atagcgtggc tttttgtccc aatcatcggt aaacacggtt tccaccgccg
    22261 accagaagtt attcagcagt ttcaccatct tcacttcatc ccccaaacct gtcgacgggt
    22321 cacggaatcg atacaatgct ccctcacgaa gactggcatc gatcatttta atcatcgagt
    22381 tgtcagcaat aaccccatca ggattggttg ccgttttgat acgtcctgca agtggtcctt
    22441 cgccgaagtt tagtcgttga gtcagcagtg aaggaaactt acgcatttgc agatcggatg
    22501 gcaaacgacc atgggtatgt ggtgccaatt cgtataacaa cgttttgggc agaggttttg
    22561 tggagttaac cagcataaac tgctcgcgtt gctcttctgc atcattagcg ataaatgcag
    22621 agaccggcat cataaacgag tcgatttccg catcccgcaa agcagcggta cgttgttgac
    22681 catccacaat aaacccaggc ttctcaaaat ccttatcttc ggagaaaggt atcaccagat
    22741 gccccatgtc gccattatct gtaagcggtt caaaacgcac acgcttatca aaagcgataa
    22801 tcaccgggtt gggtatcatc ggatttttac tctcaatata gcgctgaatt tcgcgaatat
    22861 ggttacgtac ttctggacgc tgataaccaa ccaaattctc ttcaccgcga cgaatacgcg
    22921 aaatcgctgc aattttgcta atctgtttac cttctatcgc aaggctatag agttgcctct
    22981 cctcaccctg tcggattcgc agggcgggaa cacgatattc actcatgaca gttcctctga
    23041 gcgtaatgac aatgtgtgct gtaaacggtt gtacaacacc tgaatattat ggaaaccgcg
    23101 gcgacggtta cgttctgctc cgcggaaaat gatgacgttg ataccgatag cttggcaaat
    23161 attgcactgg cattgtttcc agggacgttc acccagtgtg cgttcataat ccgcacggat
    23221 tttattggcc tctttttcac ctgaatgcag gcgttcataa gccagtactg cctcaagagt
    23281 tttttcaagg ggcaaagccc ctttatcgta ttcgaacaag gcattcatgg catccttttc
    23341 cagatgccga gcaatatcct gatcaatcac gccggatttt attttgcgac tcaaacttgg
    23401 attcgcatca aactgaggaa ctcgcacagc ggtataggcc gggccatcag gcgtatggta
    23461 gttatttttt cgatctttga aggcctgctg cagcggtgtt gtcgagtcaa tactggtcac
    23521 accaaacttg ataaagtcgg cgaaactctc tggacgggca ataccaagca agtgaacacg
    23581 tgtatcactt ttcagcagcg gtttaatttc ttcgagggtc tcaaggatct gcgtcgtttt
    23641 caacggtacc atccccccca tggtgatatt tttgtatccc atggctagca gggcctctac
    23701 agactgacgg taggagtttt tattccatcc atgtgcaaca ccaaagggag tgaaacagga
    23761 cttttgagac ttgtttaaaa actcttgtgc cagggtcaga gtgatatcct ggcgtcggcg
    23821 acattcagtc aatacttcgc cactgaatac ttcccctggc ctctcataac caaagacgat
    23881 gtgatcgaga gaaacgccat agttaaagcg ggaagtttcg tagaactcaa tcacctcttc
    23941 cacacggta
//
LOCUS       DADSZD010000064        23738 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.064, whole genome shotgun sequence.
ACCESSION   DADSZD010000064 DADSZD010000000
VERSION     DADSZD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.064"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            33..398
                     /locus_tag="J1Z28_002235"
     CDS             33..398
                     /locus_tag="J1Z28_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBA7717066.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            547..1065
                     /locus_tag="J1Z28_002236"
     CDS             547..1065
                     /locus_tag="J1Z28_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBA7717067.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1055..2281
                     /gene="dgcN"
                     /locus_tag="J1Z28_002237"
     CDS             1055..2281
                     /gene="dgcN"
                     /locus_tag="J1Z28_002237"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBA7717068.1"
                     /translation="MMDNDNSLHKRPTFKRALRNISMTSIFITMTLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTHKEPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAENLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2297..2779
                     /locus_tag="J1Z28_002238"
     CDS             2297..2779
                     /locus_tag="J1Z28_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBA7717069.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2856..3203)
                     /gene="rplS"
                     /locus_tag="J1Z28_002239"
     CDS             complement(2856..3203)
                     /gene="rplS"
                     /locus_tag="J1Z28_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBA7717070.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3245..4012)
                     /gene="trmD"
                     /locus_tag="J1Z28_002240"
     CDS             complement(3245..4012)
                     /gene="trmD"
                     /locus_tag="J1Z28_002240"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBA7717071.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4043..4591)
                     /gene="rimM"
                     /locus_tag="J1Z28_002241"
     CDS             complement(4043..4591)
                     /gene="rimM"
                     /locus_tag="J1Z28_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBA7717072.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVIDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4610..4858)
                     /gene="rpsP"
                     /locus_tag="J1Z28_002242"
     CDS             complement(4610..4858)
                     /gene="rpsP"
                     /locus_tag="J1Z28_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBA7717073.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(5107..6468)
                     /gene="ffh"
                     /locus_tag="J1Z28_002243"
     CDS             complement(5107..6468)
                     /gene="ffh"
                     /locus_tag="J1Z28_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBA7717074.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6560..7426
                     /locus_tag="J1Z28_002244"
     CDS             6560..7426
                     /locus_tag="J1Z28_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBA7717075.1"
                     /translation="MQRLEQRSPDAILLLFLIAQTVDITMPVFALLALVAYSVSLALI
                     VPGLLQKNGGWRRMAIISAVIALVCHAIALEARILPDGDSGQNLSLLNVGSLVSLMIC
                     TVMTIVASRNRGWLLLPIVYAFALINLALATFMPNEYITHLEATPGMLVHIGLSLFSY
                     ATLIIAALYALQLAWIDYQLKNKKLAFNQEMPPLMSIERKMFHITQIGVVLLTLTLCT
                     GLFYMHNLFSMENIDKAVLSIVAWFVYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLA
                     YFGSRIFQQLIS"
     gene            7447..8733
                     /locus_tag="J1Z28_002245"
     CDS             7447..8733
                     /locus_tag="J1Z28_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBA7717076.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQVLMMPLVWLLNAITRILMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8788..9381)
                     /gene="grpE"
                     /locus_tag="J1Z28_002246"
     CDS             complement(8788..9381)
                     /gene="grpE"
                     /locus_tag="J1Z28_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBA7717077.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            9504..10382
                     /gene="nadK"
                     /locus_tag="J1Z28_002247"
     CDS             9504..10382
                     /gene="nadK"
                     /locus_tag="J1Z28_002247"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBA7717078.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            10468..12129
                     /gene="recN"
                     /locus_tag="J1Z28_002248"
     CDS             10468..12129
                     /gene="recN"
                     /locus_tag="J1Z28_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBA7717079.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETACALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12278..12619
                     /gene="bamE"
                     /locus_tag="J1Z28_002249"
     CDS             12278..12619
                     /gene="bamE"
                     /locus_tag="J1Z28_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBA7717080.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(12681..12971)
                     /locus_tag="J1Z28_002250"
     CDS             complement(12681..12971)
                     /locus_tag="J1Z28_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBA7717081.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(12961..13437)
                     /gene="ratA"
                     /locus_tag="J1Z28_002251"
     CDS             complement(12961..13437)
                     /gene="ratA"
                     /locus_tag="J1Z28_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBA7717082.1"
                     /translation="MILFVGFLLMEIVMPQISRTALVPYSAEQMYQLVNDVQSYPQFL
                     PGCTGSRILESTPGQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGG
                     WKFTPLSQDACRIEFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            13569..14051
                     /gene="smpB"
                     /locus_tag="J1Z28_002252"
     CDS             13569..14051
                     /gene="smpB"
                     /locus_tag="J1Z28_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBA7717083.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14266..14628
                     /gene="ssrA"
                     /locus_tag="J1Z28_002253"
     tmRNA           14266..14628
                     /gene="ssrA"
                     /locus_tag="J1Z28_002253"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            14752..15234
                     /locus_tag="J1Z28_002254"
     CDS             14752..15234
                     /locus_tag="J1Z28_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717084.1"
                     /translation="MEDLHFVYINANGRIDVHSIQSISYSENHIQGICKNTDRIKTFR
                     KDRILKQYDSPEQAIQECASFLPENYSHLTKQSGTKKNTFDVCFTGFKKADKERLVDK
                     ANEQGLTVRTSVTQSLQMLCCGYNAGPSKVSAARMKGTIIIDEPGFIHFLETGEIPDE
                     "
     gene            complement(15261..15479)
                     /locus_tag="J1Z28_002255"
     CDS             complement(15261..15479)
                     /locus_tag="J1Z28_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="levansucrase regulator"
                     /protein_id="HBA7717085.1"
                     /translation="MMNCPKCGHAAHTRSSFQVTESTKERYCQCQNINCGSTFVTHET
                     VVRFIVTPALIATAPPHPLPGGQGHMNF"
     gene            complement(15548..16648)
                     /locus_tag="J1Z28_002256"
     CDS             complement(15548..16648)
                     /locus_tag="J1Z28_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HBA7717086.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLIGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHNTTIGEVVKEIAARHKLKMALGKELSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSATGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRGNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSIELEVKIDDLEME"
     gene            complement(16645..17130)
                     /locus_tag="J1Z28_002257"
     CDS             complement(16645..17130)
                     /locus_tag="J1Z28_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717087.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            complement(17127..20204)
                     /locus_tag="J1Z28_002258"
     CDS             complement(17127..20204)
                     /locus_tag="J1Z28_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7717088.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRAAQASSKELAGAIQNTRNSLK
                     ELNKQAGRIDEFRKTRSQLAITANNLNAAREEAAKLATQFAATNRPTAAQAKLFSQAK
                     TRVQELQQTYNGLLGAVQRQRQALKESGIDTRQLSSAQRELKKNAEETRQALEGQQKA
                     LKRLGEQQARMNAAREQYSRRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWENQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSITGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLVAAGTVLRVLASGPLAMLRVALYAVSGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPAKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            complement(20197..20316)
                     /locus_tag="J1Z28_002259"
     CDS             complement(20197..20316)
                     /locus_tag="J1Z28_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HBA7717089.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            complement(20331..20633)
                     /locus_tag="J1Z28_002260"
     CDS             complement(20331..20633)
                     /locus_tag="J1Z28_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBA7717090.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            complement(20688..21203)
                     /locus_tag="J1Z28_002261"
     CDS             complement(20688..21203)
                     /locus_tag="J1Z28_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HBA7717091.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            complement(21213..22385)
                     /locus_tag="J1Z28_002262"
     CDS             complement(21213..22385)
                     /locus_tag="J1Z28_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HBA7717092.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDNKAVATELLGVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNADATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAPMWAVDKPLNPSLARDIIEGIRAKMRSLISQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            complement(22492..22905)
                     /locus_tag="J1Z28_002263"
     CDS             complement(22492..22905)
                     /locus_tag="J1Z28_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA7717093.1"
                     /translation="MNASYAVIENGMVVNVIVWDGEAEFTVPDVQQLIDISDISEQPG
                     IGWVYSDGGFTAPPTQERSHDELVADAEQKKQSLLDAAMANISVIQLKLQAGRKLTQE
                     ETTRLNVVLDYIEAVTATDTSTAPDIIWPVFPASR"
     gene            complement(22905..>23738)
                     /locus_tag="J1Z28_002264"
     CDS             complement(22905..>23738)
                     /locus_tag="J1Z28_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717094.1"
                     /translation="AVKAVYDLANGKQPADATLTALAGLATAADKLPYFTGNDTASLT
                     TLTNVGRNILDKASTQAVIQYLGLSDASGYVGRWLNTRVFTSSGTYTPTPGTKRIRVT
                     ITGGGGGGGGCKAISNNETFFGAGGGAGGTIISIMTPTQNSYPVTIGAGGAGGVSATY
                     GINGGNSLFASLIAPGGAGGGKSGVTNTNGGNGGVPSTGDINIIGGCGGDGQSGNIGV
                     SGEGGTSYWGGGGRAGAGGGVSGKAYGSGGGGAYDAGYSGTSMTGGKGAAGICIIEEF
                     A"
BASE COUNT         5676 a         6517 c         5661 g         5884 t
ORIGIN      
        1 caatccgcta tgctttgaaa aaggagaaag aaatgatgaa aaaatttatc gcccccttgt
       61 tggctttact ggttagcgga tgtcagattg atccttatac tcacgcgcca accttgacca
      121 gcaccgactg gtatgatgtt ggtatggaag atgcgatatc gggcagcgcc attaaagatg
      181 acgatgcatt tagcgattca caggcggatc gcggtctata ccttaaagga tatgccgaag
      241 ggcaaaagaa aacttgccag accgatttta cttatgcccg gggactttct ggtaaaagct
      301 ttcctgccag ctgcaataat gttgaaaatg ccagccaact gcatgaagta tggcgaaaag
      361 gggctgatga aaatgccagc acaatacgat taaattaatt acccccccaa aatcaattaa
      421 gcatgagatt ttactcatta tgttttattt ctcacattga tgacgaccgc atgaaaggat
      481 gatattttaa tgagcattct gaaaaaatat cgatgcattt cgagcgaaga tggtgaggat
      541 ccctgaatgc gtttttctca ccgactgttc ttactgctga ttctcctgct gacgggagca
      601 ccaatactcg ctcaggaacc ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt
      661 attgtcagtt acacccgctg gcctgggcta tcagggccac caaagttatg tatcttttca
      721 tcttcacgtt ttagtaccgc cttacaagaa aatgctgcta catctttacc ttatctgcct
      781 gtcatcattc atactcaaca agaggcgata atttcaggct gcaatggttt ttattttggc
      841 aacgagtcac cgacattcca gatggaatta accgaacaat atccgtcaaa ggcgttatta
      901 ttaattgccg aacaaaatac cgagtgcatt attggtagcg ccttttgtct gatcatccac
      961 aataatgacg tcagatttgc cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc
     1021 aacccggatg tcttaatgct cgcacggaag aaaaatgatg gataacgata attctcttca
     1081 taagcgcccc acgtttaaaa gagcattacg caacatcagc atgaccagca tatttatcac
     1141 tatgacgctg atctggctgc tgctttccgt gacgtcagtg ctaaccctga aacaatacgc
     1201 gcaaaaaaac ctggcactga cagcagcaac aatgacttac agtctggaag cagctgtcgt
     1261 ttttgccgat ggccctgcag caactgaaac actggcagcg ctgggccagc aaggccaatt
     1321 ttcaactgca gaagtgcgtg ataagcagca aaatattctg gcatcctggc attacaccca
     1381 taaggaacca ggcgatactt tcagcaattt cataagccac tggctcttcc ctgcccccat
     1441 cattcagccg attcgtcaca atggtgaaac cattggcgaa gtacgcttaa ctgctcgcga
     1501 cagttcaatc agccatttta tctggttttc gctcgctgtc ctgaccggtt gtattctgct
     1561 ggcatcaggc atcgcaatta ccctcacccg ccatttgcac aatggcctgg tggaagcgct
     1621 gaaaaatatc accgatgtcg tacatgatgt gcgttctaac cgcaattttt cccgacgagt
     1681 ttcggaagaa cgtatcgctg agtttcaccg cttcgctctc gacttcaaca gtctgctgga
     1741 tgaaatggaa gagtggcagc ttcgtttaca ggccaaaaat gcgcagcttc tacgtaccgc
     1801 gctacatgac ccattaaccg ggctggctaa ccgcgcagcg tttcgtagcg gcatcaacac
     1861 gttgatgaac aattccgatg cccgaaaaac gtcggcgtta ctatttcttg atggcgataa
     1921 tttcaaatat atcaatgata cctggggtca tgcgacgggc gatagagtct tgattgaaat
     1981 agcaaaacgg ttagctgaat ttggcgggct gcgacataaa gcataccgcc tgggcggcga
     2041 tgaattcgct atggtgctct atgatgtaca gtcggaatct gaagtgcagc agatatgctc
     2101 agcactgaca caaattttta atctcccgtt tgatcttcat aatggccatc agactaccat
     2161 gacattaagc attggttacg cgatgaccat tgagcacgcc tctgcggaaa atttacaaga
     2221 gcttgccgat cacaatatgt atcaggccaa acaccagcgt gccgaaaagc tggtgagata
     2281 acaaggatat atcgatatga taaagcacct gctagcaccc ctgattttca cctcgctaat
     2341 acttactggc tgccagtccc ctcagggaaa gtttactcct gagcaagtcg ccgctatgca
     2401 atcttatgga tttactgaat ccgccggcga ctggtcgctg ggcttatcag atgccattct
     2461 gttcgcaaaa aatgactaca aattgctccc ggaaagccag caacaaatcc aaaccatggc
     2521 agccaaattg gcctcgacag ggctaacaca tgcccgtatg gatggacaca ccgacaacta
     2581 tggtgaagac agttacaacg aagtcttatc attaaaacgg gcgaatgtcg tggccgatgc
     2641 atgggctatg ggtggacaaa ttccgcgcag caatctcacc acacagggtt taggaaaaaa
     2701 atatcccata gccagtaaca agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt
     2761 ggtgattaca accccttaaa acacgagcaa acagacaaaa aaagggccag ccaattggcc
     2821 agcccttctt aacaggatgt cgcttaagcg aaatcttagt taagacgctc tttgatacga
     2881 gcagccttac cagtacgctc acgcaggtag tacagtttag ctttacgaac agcaccacga
     2941 cgtttgacag aaatgctgtc aactaccgga gagtgagtct ggaagacacg ctcaacgcct
     3001 tcgccgttgg aaattttacg aacagtgaat gcagagtgca gaccgcggtt acgaatagcg
     3061 ataaccacgc cctcgaatgc ctgcagacgt tttttggaac cttcaacaac ccatactttc
     3121 acttccacgg tatcacccgg acggaaggaa ggtacgtcct gcttcatctg ctcttgttca
     3181 agttgcttaa taatgttgct cataatttaa tctcttatcc tgggtaaact gatatctcgg
     3241 gggcttacgc catcccatca tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg
     3301 ccagcaacct tgcttgctct tcagtcagag ccaggttttc cagaagttca ggtcttctaa
     3361 gccaggtacg gcccagcgac tgtttcaaac gccagcgacg tatctcggca tggttgcccg
     3421 acagtaacac tggcggaact tccatccctt ctaacacctc aggccgcgta tagtgcgggc
     3481 aatccagcaa tccttcagca aaggaatctt ccgttgctga ggcttcatgt cccagtactc
     3541 ccggaataaa ccgggaaacg gagtcaatca gcgtcattgc tggtaactca ccaccactga
     3601 gaacgtaatc gccgattgac cattcttcgt caatttcggt ttggatcacg cgctcatcta
     3661 taccttcgta gcgaccgcac accagaatca atttttgatt cgttgccagc tcgctgacgc
     3721 ccgcttgatc aagcttgcgt ccctgtggtg acagataaat cacctttgcg ccttcacccg
     3781 ccgcggcttt tgctgcatga atggcatccc gcaagggttg caccatcatt aacatccccg
     3841 gtccgccgcc gtaaggacga tcgtccacgg tacggtgccg gtcatgtgtg aagtcgcgag
     3901 gactccagct ctggatgctc agcaggccat ttttaactgc ccggccagtt accccgtaat
     3961 cggtaattgc gcggaacatt tcaggaaaca ggctaattat gccaatccac atagcgccgt
     4021 cttttaccgt ttatccggtg gtttaaaaac caggatccca atctacttcg attgaacgag
     4081 tagtgagatc gactttcttg ataacctgcc catcgaggaa cggtacgaga cgttccttga
     4141 taccaaacgc atctttcagg tttgccttaa tgacgagaac gtcattagat ccggtttcca
     4201 tcatatcgat gactttaccg agatcgtagc cttcagtggt tactacctgg cagcccatca
     4261 ggtctttcca gtagtagtca ccctcttcaa gctgaggcaa ctgcgatgaa tccacgacaa
     4321 tttcacaatt cgtcagcagg ttcgccgcat cacgatcgtc aacgcctttc agcttgataa
     4381 tcatgtcctg attgtggtgc ttccagcttt ccagctggac ttgctgccac tgacccgcct
     4441 tctggataaa ccagggctga tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca
     4501 ctctgagcca cccacgaata ccgtaagacg accccatttt tcccaaaacg atgggatcaa
     4561 caggtgcttg cgcggtgagt tgtttgctca tcatgaccac cgtgacagat taagctgctt
     4621 tgtttacttc tttgatcagc gcagcaacgc gatcagaaat agttgcgccc tggccaaccc
     4681 agtgagcgat gcgatccaga tccaggcgag tgccttcttc tttttcgcta gcgattgggt
     4741 tgaagaaacc aacgcgctcg atgaagcgac cgttgcgtgc attacggctg tcagcgacaa
     4801 caacctggta gaacggacgc tttttagcgc cgtgacgtgc taaacgaata gttaccataa
     4861 catcctcttg tgtgaataaa acaaccggac cccatcgagg aacggagtcc ggtgtcatat
     4921 taaaagcccg aaaattttac tcatttttgc ggaaattgca atcaacagtt gctaactctg
     4981 ctgtaaaagg ccgtcggcgg tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag
     5041 cgtacacgca gtacgtgagg atgacgagca caccccggtg ccaaaatggc aaacaagcca
     5101 ggccgattag cgaccaggaa atcccggcgg catcatccct ttcatgcttc tcatcatctt
     5161 cgccatcccg cccttcttca ttttcttcat catgcgctgc atgtcgtcga actgcttcag
     5221 aagacggtta acgtcctgca cctgcatacc gcaaccggca gcgatacgac gcttacgcga
     5281 acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga tgatggcttc
     5341 catacgcacc agcactttat cgtccatctg cgacttgacg ttatccggga tctgccccat
     5401 gcccggcaac ttgcccatca ggctagccat gccgcccata tttttcatct ggcgcagctg
     5461 ctcaagaaag tcgttgagat cgaagccgtc gccttttttc agcttgctgg ctaatttctc
     5521 tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagca cgtcgcccat
     5581 gccaagaata cgggaggcaa tacggtccgg atggaatggc tccagcgcct cggttttctc
     5641 gccaacaccg aggaacttga tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc
     5701 gccgcgggca tcgccgtcca ctttggtcaa cactacaccg gtaagcggta acgcttcatt
     5761 gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcatcgacca caaacagggt
     5821 ttcaaccgga ttaatcgacg catggacttg tttgatctcg tccatcatcg cttcgtcaac
     5881 gtgcagacga ccggcagtat ccaccagcag cacgtcgtag aatttcagtt tggcttcttt
     5941 cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga agaaatcaac
     6001 gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat agacgtcggc
     6061 agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac
     6121 gctggttgtt ttaccggcac cttgcaggcc cgccatcagc acgaccgcag gcggttgcgc
     6181 agccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt tacggacaat
     6241 tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa ccgctttctc
     6301 ttttacgcga ttgataaact cacgcactac cggcagagcg acgtccgcct ccagcagcgc
     6361 catgcgcact tcgcgcagcg tatcttttac gttgtcttca gtgaggcgtc cacggccact
     6421 gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg tctctcgcct
     6481 ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg cagtataaca
     6541 tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg ctatactgct
     6601 tctctttctt attgctcaaa ctgtcgacat tactatgccc gtttttgctc tgctcgcgct
     6661 tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg
     6721 ctggcggcgc atggctatta tttctgcggt catcgcgctg gtctgccacg caatcgctct
     6781 ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc tgaacgttgg
     6841 ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg
     6901 ctggttgttg ctacccattg tctatgcctt tgcgcttatc aacctggcgc tggcgacctt
     6961 tatgcccaat gaatacatca cccatctgga agcaacgccg gggatgctgg tgcacattgg
     7021 cttatcgctt ttttcctatg ccacgctaat tatcgctgcc ctgtacgcgc tgcaactggc
     7081 gtggattgat taccaactga agaacaagaa gctggcgttt aaccaggaaa tgcctccatt
     7141 gatgagtatc gagcgtaaaa tgttccatat cacgcagatt ggcgtggtgc tgctaacgct
     7201 cactctttgc actggcctgt tctacatgca caacttattt agcatggaaa atatcgacaa
     7261 ggccgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg gacattatca
     7321 tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggtgcag tcattctgac
     7381 actggcctac ttcggcagcc gaatttttca gcagttaatc agctaacccc agaaaaggag
     7441 tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat catggtggtc
     7501 atttcagcct atttttccgg gtccgaaacc ggaatgatga ctctcaaccg ctatcgtctt
     7561 cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag
     7621 ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc
     7681 gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc aattgcgact
     7741 ggcgtgctga ccttcgtagt gctggttttt gctgaagtat tgccgaaaac cattgccgca
     7801 ctgtacccgg aaaaagtcgc ctatccgagt agttttctac tggcgccgct acaagtcctg
     7861 atgatgccgc tagtctggtt actaaatgcc atcacccgga ttttgatgcg tatgatgggt
     7921 attaaaaccg atatcgtagt cagcggctct ttgagtaaag aagagttgcg caccatcgtg
     7981 cacgaatcgc ggtcacaaat ttcacgtcgc aatcaggata tgctgctgtc ggtgctcgat
     8041 ctggaaaaga tgaccgtcga tgacatcatg gtgccgcgca gtgaaattat cggtattgat
     8101 atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca cgggcgcatc
     8161 gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgtgtgcg tgaagcctgg
     8221 cgactcatgt cggagaaaaa agagttcacc aaagaaacca tgctacgcgc cgcggacgag
     8281 atctattttg tcccggaggg tacgccgctc agcacacagt tggtgaagtt tcagcgcaac
     8341 aaaaagaaag tcggactggt cgtcaacgag tatggagaca ttcagggact ggtgacggtc
     8401 gaagatattc tcgaagagat tgtcggcgat ttcaccacgt cgatgtcgcc aacacttgcc
     8461 gaagaggtca cgccgcaaaa cgacggttcg gtgattattg atggcactgc caacgtgcga
     8521 gaaattaaca aagcctttaa ctggcatcta ccggaagatg atgctcgtac tgttaacggc
     8581 gtcattctgg aagcgctgga ggagatcccg gtagcaggca cccgcgtgcg tataggcgag
     8641 tacgatatcg atattctcga cgtacaggac aatatgatta agcaggtaaa agtttttcct
     8701 gtaaaaccgc tgcgcgagag tgtggcggag taacgaaaac ggcccggcat tcgcatgctg
     8761 ggccgtgatt tattacgaaa gcagaaatta agcttttgct ttcgctacag tcaccatcgc
     8821 cgcacgaatc gtacgaccgt tcagcgtata acctttctgc ataatgccca gtacgttacc
     8881 tggcgcaacg tcatcagatt ccaccattgc gatggcctga tgcacatttg ggtccagtgg
     8941 gacgttggtt tcggcgatca cttcaacgcc aaacttacgc acaacatcca gcatcgactt
     9001 cagcgtcagc tcaatacctt caaccatcgc ggacatatcc gggttagctt tatcagccac
     9061 ttccagcgca cgatccaggc tatcaatcac cggcagcaat tcgttgatga atttctccag
     9121 cgcgaatttg tgggcttttt cgatatccag ttcagtacga cgacgcaggt tttccatttc
     9181 ggcttttacg cgcaaaatgc cgtcacgttc acgggtctgg gcttcagcca gctgagcttc
     9241 gagattcgca attttttcat cgcgcggatc cacctgctca gcagaagctt ctggctcaac
     9301 tgcctcaatc tcttcgtgct gatccatgat aatttcttcc ggggcttgcc cctcaggcgt
     9361 tttctgttct ttactactca tgaatttctc cgcgtttttt tcgcattcat ctcgctaact
     9421 tcgcttatta tggggatcag tttcagggtt tcaagggaag cactcacatt gtcatcaatc
     9481 ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa gtgtattggc attgtgggac
     9541 acccacggca ccccactgca ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa
     9601 aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga actgcaactg aagaatgtga
     9661 aaactggcac gctcgcggag attgggcaac aggctgatct cgcggttgtc gttggcggcg
     9721 acggtaatat gctgggcgcg gcgcgcacgc tcgcccgcta cgatattaaa gttattggaa
     9781 tcaaccgtgg caacctgggt ttcctgactg accttgatcc cgataacgcc cagcaacaat
     9841 tagccgatgt gctggaaggc cactacatca gcgagaaacg ttttttgctg gaagcgcaag
     9901 tctgtcagca agattgccag aaacgcatca gcacggcgat taacgaagtg gtacttcatc
     9961 ctggcaaagt agcgcatatg attgagttcg aagtgtatat cgacgagatc tttgcgtttt
    10021 cgcagcgttc cgatggactg attatttcga cgccaacagg ctccaccgca tattccctct
    10081 ctgcgggcgg tcctattctg acaccctctc tggatgcgat taccctggtg cccatgttcc
    10141 cgcatacgtt gtcagcacga ccactggtca taaacagcag cagcacgatc cgtctgcgtt
    10201 tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag ccagatagca ctgccgattc
    10261 aggaaggtga agatgtcctg attcgtcgct gtgattacca tctgaatctg attcatccga
    10321 aagattacag ttatttcaat acattaagca ccaagctcgg ctggtcaaaa aaattattct
    10381 aattttacgc cagcctcttt actgtatata aaaccagttt atactgtaca caataacagt
    10441 aatggttttt catacaggaa aacaactatg ttggcacaac tgaccatcag caactttgct
    10501 atcgttcgtg agcttgagat tgattttcat agcggcatga ccgtaataac tggcgagacc
    10561 ggcgcgggta aatctattgc aatagatgcc ctcggccttt gtctcggtgg tcgcgctgaa
    10621 gccgacatgg tgcgtaccgg cgctgctcgt gccgacctgt gcgcccgttt ttctctgaaa
    10681 gatacgccag cggccctgcg ttggctggaa gaaaaccagc ttgaagacgg gcatgaatgt
    10741 ttgcttcgtc gcgtaatcag cagcgatggt cgctcccgtg gtttcatcaa cggtacagct
    10801 gttcctctgt cacaactgcg cgaactgggt cagttgctga ttcagatcca tggtcagcac
    10861 gctcatcagt tactcaccaa acccgagcac caaaaattcc tgcttgatgg ctatgccaat
    10921 gaaacctctc agctccagga aatgaccgca cgttatcatt tgtggcatca aagctgccgt
    10981 gacctcgcgc atcatcaaca gttaagtcag gaacgcgccg ctcgtgcgga gctgctgcaa
    11041 taccaattaa aagaacttaa cgaatttaat ccgcagcctg gagagtttga gcaaatcgac
    11101 gaagagtaca aacgtctggc gaacagcggt caattgctga ccaccagcca gaatgcattg
    11161 gcattaatgg ctgacggtga agacgcaaac ctgcaaagtc aactttacac ggctaaacaa
    11221 ctggtgagcg aattgattgg catggacagc aaactgtccg gcgtacttga tatgctggaa
    11281 gaggctacca tccagattgc tgaagccagt gatgaactgc gccactactg cgatcgtctg
    11341 gatctcgatc ccaaccgact gtttgaactt gaacagcgca tctcaaaaca gatttcgctg
    11401 gcacgtaaac atcacgtcag ccctgaggca ttgccacagt attaccagtc gctactggaa
    11461 gaacagcagc aactggacga tcaggccgac tcacaagaaa cgctcgcgct ggcggtaacg
    11521 aaacatcatc agcaggcact ggaaacggcg tgcgcgttac accagcaacg ccagcattat
    11581 gcaaatgagt tggcacagct gatcaccgat agtatgcatg cactctctat gccacatggg
    11641 cagtttacga tcgatgttaa atttgacgag catcacctgg gcgctgacgg cgctgatcgt
    11701 attgagtttc gggtaaccac caacccaggt cagccaatgc agcctattgc caaagtcgca
    11761 tccggtggtg agttgtcccg catcgcattg gcaatccagg tcattaccgc gcgtaaaatg
    11821 gaaaccccgg cactgatttt tgatgaagtg gatgtaggga ttagcggccc aacagcggca
    11881 gttgtcggca aactgctacg tcaacttggc gaatcaactc aggtgatgtg tgttacccac
    11941 ctgccacaag tcgcgggatg tggtcatcaa cactattttg tcagcaaaga aaccgatggt
    12001 gcgatgacag aaacgcatat gcaatccctg gataaaaaag cgcggttaca agagttggcg
    12061 cgcctgcttg gtggcagtga agtcacacgt aacacactgg cgaatgcgaa agaactgctt
    12121 gcggcgtaaa cttttttcct gcttcacggt cagagtaaac agaaaaacgc cgtaagaccg
    12181 gaaagcaaaa ggttttaaag tgatgaaagg tctattatca tcggcatatt acagatgagc
    12241 cacgtactgc tcgggcccga aaaggaatca aatcactatg cgctgtaaaa cgctgactgc
    12301 tgcagcagca gtactattga tgttgaccgc aggctgttcc actctggagc gagtggttta
    12361 ccgtcctgac atcaaccagg ggaactatct gaccgctaac gacgtatcca aaatacgtgt
    12421 tggcatgacg caacaacaag ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt
    12481 tggtacaaat acctggttct atgtcttccg ccagcaacct ggtcatgaag gtgtaacgca
    12541 gcaaacgctg acgctgacct ttaacagtag cggtgtgttg accaacattg ataacaaacc
    12601 tgcgctgagt ggtaactaat aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat
    12661 gagcaccttt tttctgtctg ttatttattc gctgattttt ctgctcgttg cctgcgaagc
    12721 tctttcggat cggcaatgag aggacgataa atctccaccc gatcgccatc atgcacaata
    12781 tcgctgagtt ttgccggacg gctgtaaatg ccgactttgt ttttagttaa atcgatatcg
    12841 gtacgcaatt ccagcaaacc actggcgcga atagcttctt caaccgtcgc gccctcctgc
    12901 aacgtcacgc gctgcaggta ctgcttctca ggtagcgcat aagccacctc aacggcaatt
    12961 ttacctggca ctgtagacct cttttgcacg aacagtaaaa gcctgcacca tattagctgc
    13021 cagctcttta aacacacgac caaaggcgag ttcaatcaac ttattggtaa actcaaagtc
    13081 gagatgaaac tcaatacggc acgcgtcctg gctcaacggc gtaaacttcc aaccgccgat
    13141 caatttctta aacggtccat caaccagact catgagaata ctttgattac tggtcaactg
    13201 gttgcgggtt gtaaacgttt tgctgatccc agccttagac acatctaccg cagcagtcat
    13261 ctgcccagga gtggactcca gaatccgact tccggtacaa cccggcaaaa actgaggata
    13321 agactgaacg tcattcacta actgatacat ttgctccgcg ctgtagggca ccagtgcggt
    13381 ccgactaatc tgaggcataa caatttccat caacaaaaat ccaacaaata atatcattta
    13441 tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca tcgggttcat gctaagatag
    13501 agccttgtcc cccgcaggat tgatatgggg tgttttcgat ttcagattac cgatgattca
    13561 cgacgcttat gacgaagaaa aaagcacata aacctggttc agcgaccatc gcgcttaaca
    13621 agcgcgcccg tcacgaatac tttatcgaag aagagttcga agcgggactt gccctgcaag
    13681 gctgggaggt taaatccctg cgcgcaggaa aagccaatat cagcgacagc tatgtccttc
    13741 tgcgcgacgg agaggcattt ctgtttggcg ctaacatcac gccaatggct gtggcctcca
    13801 cgcatgtggt gtgcgatcct acccgtaccc gcaagttact tctcaaccag cgcgaactgg
    13861 actcattgta cggtcgcgtc aatcgagaag gctataccgt agtggcgctc tccctgtact
    13921 ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa aggtaagaaa cagcacgata
    13981 aacgttcaga tatcaaagaa cgcgaatggc aggtggataa agcgcgtatc atgaaaaatg
    14041 cccaccgtta aacctatata agcgatacta atcaaatcct caccgcgcct ccctatccgg
    14101 tggcgcgaat gaacatctta tcgactgtca aatctgacac agatgtagcc atcccattgc
    14161 ttaatcgaaa aaaaatcata ctatgcaggc gctaattcca ggatgataac gccttttggg
    14221 ctagtcatgc tgctcataaa tctggtatac ttaccgttac acattggggc tgattctgga
    14281 ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc
    14341 cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc agcttaataa cctgcttaga
    14401 gccctctctc cctagcctcc gctcttagga cggggatcaa gagaggtcaa acccaaaaga
    14461 gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta
    14521 gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac tgactaagca tgtagtaccg
    14581 aggatgtagg aatttcggac gcgggttcaa ctcccgccag ctccaccaat taaaacaagg
    14641 ggttacgtga aagcgtagcc cctttttctt tggtagtggc ggcaaaatgg cgacagacgt
    14701 ttgcgtccat cttgcctgtc gccatcttga aatcatgcaa agaggtttca catggaagac
    14761 cttcactttg tttacatcaa tgcaaatggt cgtatcgatg ttcactccat acagagcatc
    14821 agttatagcg aaaatcatat acagggcatt tgtaagaaca ccgatcgaat aaaaaccttc
    14881 cgaaaagacc gcattcttaa acagtacgat tcaccagaac aagccattca ggaatgcgcg
    14941 tcattcctcc ccgaaaacta ctcacatctc actaagcagt ctggtacgaa aaaaaataca
    15001 ttcgatgtgt gtttcaccgg atttaagaaa gcagataaag aaagattggt tgataaggcg
    15061 aatgaacaag gattaacggt aagaacctct gtaacccaaa gccttcagat gctctgttgc
    15121 ggttacaatg caggcccatc aaaagtatcg gcagccagga tgaaaggcac aatcatcata
    15181 gatgagcctg gctttataca ttttcttgaa acgggtgaga tcccagatga ataaaaacct
    15241 gccgtagcag gttctctttc tcaaaaattc atatgcccct gaccacctgg caatggatgt
    15301 ggaggagcag tagcaatcag tgcgggtgtc acaataaacc ggaccactgt ttcatgggta
    15361 acaaaagtgc tcccgcagtt aatattttgg cactggcagt aacgctcttt ggtgctttca
    15421 gttacttgaa aactgctcct tgtgtgtgcc gcatgaccac actttggaca attcatcata
    15481 tccagatccc tacctttgct atcagaatca ttgtaatgat acacaaaata tcaatattga
    15541 gaacatttta ttccatttca agatcatcaa tcttcacttc gagttcaata ctggttgtaa
    15601 aaccgttatc cgggctgacg gtatgtgtca gagtcgtaat ggtccattcc gcatcatcta
    15661 tcggctgttt aaagccactg actttcactg gcatttccgt gtagagatct gcccgccctt
    15721 ccgccagttg tagcgagaat gacgcaacgc cgcgttgcag acgttcccac tgcattttcg
    15781 ctgcccgttc agcgttgccc cggttggcat aggtgcgatt aagtaccagc acgttttcat
    15841 ccgtacccac caggtaatcg ccctgcttcg cttccggctc tttcttctgc ttcttagttc
    15901 tgcgcttacg cttcaccgtg gtgctttctt tcttcgcggg ttcacgggta tgcaaccagc
    15961 tggcaattac gcccgtgtag gctccgcgat ctgccagggt aaatcggtga ctgtcgccgt
    16021 ccttgcgtgt gatagtgatc actggcagtg gtttaccagt ggcgcttttg ccctgcccct
    16081 gccggatgaa taacagattg ccatttttca ccgacgcgat ggcaccgtac tgtcgcgcca
    16141 gtcgcatcag aaaactgccg tcactctcat tagtctggtc tatatgctcc acgggcttat
    16201 ccgacagctc tttacccagt gccatcttca gcttgtgccg cgcggctatt tccttcacca
    16261 cttccccgat ggtggtgtta tgccacgatt tttcacggcg ggtattcagg gtttcccgaa
    16321 aatcagcact tcgcgcccgg atagtcaggc ggtccggtgc gccagtgtgt tcaatctcgt
    16381 ccaccgtgaa tgcccctttc gggaaaagcg gctgcccctt ccagcccagc gccagcgtaa
    16441 tgaccgcacc acggcgcggc agcacgattt ttccatcggc gtcgtccagc tccagatcaa
    16501 gctggtccgc ttcaaatccc cggttatccg tcagcgtcag cccaatcagg cggttgtcca
    16561 gcacagtggt gatatccctg ccttcaatac tgatgctgaa tgccggagtt ttgttgcctt
    16621 tgttaagcag ttcagagctg aaattcacga cagaagccct cccaccgttt tactgatatc
    16681 gcttaaggca gatgttgccg ttccctgcag attattcagc tgcgcactga gatcaccgaa
    16741 catatcggac agggattcat ccacccgttt gagcgacagg gtgaactcaa tccggcgcgg
    16801 cataccgtcg cggaaaaact ccgttttagt ctgattcagt ccctcaatca catacatgcc
    16861 gtaaatcgtg ccgctgcctt caatcagagg ccatgctttc ccctgttctg ccatctgctc
    16921 cagtgccagc aacgacagcc tgccgcctgt tatctccggc ataagaacac cggaaagcgt
    16981 cagcatgtcg ttttccggtc ccagaaactg cgtggacgga cgacgattta cccggctgtt
    17041 tgccgcatgt cgccagctgc gttgatactg cagttcctga tacggaacgg tgcgcagcat
    17101 aaacacgtac aatcccagca ccatcatcat gcgtcgtatc ccccctgatc gctgtagtta
    17161 ctcctggctt ttgccttcag cctgcgttca cgttcatcaa gctggcgtgc cacctcccgc
    17221 gcaatatcct gcgcactttg tcctggctgc gtctgaatga tgatctgcgt cggtgcctca
    17281 atccgttgaa cgagcggcac agtggctgcg cgactcacaa ttgcttctcc acctttcgcg
    17341 ggaagtgcca aagggtgcaa cggtggaagc tctgctggcg cggcagcaac gcccatcatt
    17401 ccggcaacaa cggcagccag tgcagctgta tttctccggc tggtcacatt tgccgggccg
    17461 ttaacaattt ccggcccgtt ttcaccgacg atgccaaact gcccgcgcgg gatatacccg
    17521 ccgctgtcat acatccccgc aaagccatat ccccatgacg gaaaaccacc cgatggcatc
    17581 atcactttac cgtctgcatt caccgtcgca ggttgctgac gcgtcacgct ttccggcagt
    17641 tttgcctttg cggcctcttt actgacaatg ccaagtttct ccagcaacca ggaaacgccg
    17701 gatttcaggg agtccagcgg atgcatgacc atattcagcc cttccgccag tgcctctccg
    17761 aatcgccgcc ccattgccgc tgcactctgc agttcggcag aggtcgactt aacgggcgtc
    17821 agcagatcag taaaccagcc ccacagcgcc tgtactttgt cgccaatcca ctggaacacg
    17881 ggcttaagcg gttcgaatgc tgcactgacg ggacctgccg ccgctttgaa tccttccacc
    17941 acgccaccaa gaaatgcggt gatgggttgc cagtatttcc agacaaccag cgccacgccc
    18001 gccagtgcag taaccacaag acctatcgga ctgagcagag cacctaacag accagatacg
    18061 gcatacaggg caacgcgcag catcgccagt ggaccagatg ccagtactcg cagcaccgtg
    18121 cctgcggcga ccagtccacc gcgcagtacc gccagaggat tcataaacat cacagcaaca
    18181 gcacgtaaac cggataatcc agaccgcaaa agtgcaactg gcgcacctgc tacagttttc
    18241 aggacatttc ccgtcagtga tgccgtgcgg cgcaaagacg acaacggcgc agtaagtaaa
    18301 cctgcggcgt tgcccgatga agcaagcccg cgtcgcagca gtgccagtgg tgcgccagcc
    18361 aaccaggaca acgcgctgct ggttcgagtt actgctgccg taacagaagg taacgttttg
    18421 atacccagca cagagaatcc cagacggatc actgccagcg gccccagcac tgcagccagc
    18481 gccaccgcta aggtgccgag gcctactgta accgcagcca caacagccgc tactttcatc
    18541 aatgtgcctg tcagttccgg gttagcttcc acccagcggc gcaacgcccc cgtgatgctt
    18601 ttcaccgtgt acagaatatc catcagcggc tggcgcagcg tttcgcccag gctgctgaag
    18661 gtgttctgcg ctccggtttt gaccagcaac cactgagcag aaagtgagtc tttgttgatg
    18721 tcggattctt tctgcatgga accgagcgca tcattgcccg ctgtcagttt tagctggcgc
    18781 tgcagttccg gaaggttgtt tgccagtttc gccgcgtcat caccaaactc tttaccaaac
    18841 aacatggtca tggcagacag acgcttgtcc tgcggcagtg cgttcacctt ctccagcaca
    18901 cgctggatag ttcccatcgc atccttcgtc atctgctttt caatcacttc aggattgagt
    18961 ttcagcagat tcatcccttc aaagaaactc ttgctttgca tggtggcaat ggacaattca
    19021 cgcaccatcg cgtttgctgc actggctgca acctccggcg cagcgcccag tgtcagaaag
    19081 gtggaaccca gtgccgccgc tttacgataa tccagacggt cagccacacc gcccagacgt
    19141 tgcatcacat caatgatgtc cgcccctttc gacatggcgt tatcatccag atagttcagc
    19201 gcatcgccga gctgttcaat attgcgggtt ggtattttgt agagctgggc gattttcccc
    19261 agactttctg acagttcatc cgctggcagc tcaaaggctg ttgccgcctt tgctgccgta
    19321 ctggcgaagg ccagcaggtc acgtttctgg ttttcccagc tgtcgtcagg gtttgcgacg
    19381 ttcatgcgcg caccaccttc aaccagtgca gcgaagtcca ccgcaccgtt ttccatcggc
    19441 agctgttcgc tggcagcctt gatggcatcc tgcatttcat aaaaacgtgc agtgcggttg
    19501 ccattatcgt cacgcagacc attgacctgc tttgccacac ctttcatggc atcttccatg
    19561 ctggtatagc tttttactgc cgccatcact ggcgcaccca ttgccagccc tgcagccgtg
    19621 gtagtggctc cggctcctgc gatgcgatcg cgcacttcaa gccgtcttga gtattgttct
    19681 ctggcagcgt tcatccgtgc ctgttgttca cccagacgtt taagtgcttt ttgctggccc
    19741 tccagtgcct gccttgtttc ttcagcattt ttcttaagtt ctcgctgagc actactgagt
    19801 tgtctggtat caatccctga ttctttaagt gcctgacgtt gtctctggac cgcccccaac
    19861 aagccgttat aggtctgctg aagttcctgt actcgtgttt tggcctgact gaataacttt
    19921 gcctgcgcgg cggttggcct gttagtggca gcaaattgtg tggcgagttt tgccgcctct
    19981 tcgcgggctg cgttcaggtt gttggctgtt atggctagtt gcgagcgcgt cttgcgaaat
    20041 tcatcaattc tgccagcctg cttattcagt tctttgaggc tgtttcgggt attctgaatt
    20101 gcgccagcca gctctttcga actggcctgt gcagcacgga atgggcgggt gagtttgtca
    20161 accgcattaa gaatgacctg caggcgcagg ttattatcac tcatcgttgg ccccgcttct
    20221 ctgaatcgct ttataccgcc attccagcac ttcggtcagc ggcataacgt cagtaacgga
    20281 tggcggccag tgaaagatgg tggcgatatc agccaccaga tcgtcaaccg tcaggctgtc
    20341 ggtaaaccgg caagcaccga cttcttcaac aaaaaagtga caacctcaac cgacatggca
    20401 gtgagatctg ccgggtccat ctctgcaatt tcctgtgcgg tcagtgccgg gctggagatg
    20461 cgggggatca cggtcatcat cgcgttcaca tccatatcca taatggcctg caggcgtgta
    20521 ccgcgcagcg caccggactg cggtttacgc agcacaattt cggtaatttc tgttttaccg
    20581 cgcttgatag gggtatccag ttgaatggtc ttttcagtct gcttatcgct cattttattg
    20641 tcctgtaaat tgggttctgg cgcggaatcc cgcgccgttc agatacatca gaggccgagg
    20701 gcgttgcggt gcgcttccat caggtccacg ccgtccacaa tttccaccat gttgataagg
    20761 tccacctcat agagcacctc accattgata gtcagcttcg cgtagctgtt ggtactggtc
    20821 actttggtgg tgttgctttc gcccgtcttc cactcgccgg aatccacttc tttgtgacgt
    20881 ccacgcacca caagctccac ggcctgcact tccccggtat cgtcacgctg aatagagccg
    20941 gtaaagcgca actggatacc atccaccgtg gctttgccca tctgcttaaa cagcagcagt
    21001 tcagtaccac caatggaaaa ttctgtgtcc agcgcactat catccagccc cagatccaca
    21061 tccactgcac ccggcattcc gccaccgcga tacttctcat atttgcgggt gaatttcggc
    21121 agcgtcagcg actcaacgat cccctgccag ttgttcccgt cgttaaacag gttcaggtgt
    21181 tttaatttac gtggtaaagc catgttgtcc ccttacgcgc tgacctggct ggagaaattc
    21241 accaggtact gatcggtgat gcgctgacgc agcatcaggt tttcaagtgg cggcactggt
    21301 gtgtagtcgt agtcgatggt gagttttcca gctttcagag tgtctttgtc gttcaccgac
    21361 tcatccagcc agcaatcgcc accaatgaga tagccctgac ttatcaggct gcgcattttg
    21421 gcgcggatac cttcgataat gtcgcgggcc agcgacggat tcagcggttt atcgaccgcc
    21481 cacattggcg cttctgccat cgtgtccatc agcacctgcg ccgtgcgggt gtagttttcg
    21541 aaggcaaaga gtgggtcatc actcaggcag cgggaacccc agaagcggaa accgtcttta
    21601 cgcacaagcg tggtgacgtc gttctggttc agcagacctg catcagtggc cgggtcctgc
    21661 agatcccaga acacatcagc agaaattccg gtgacaccgt tcacgcccac gttggacagg
    21721 cttttgtgcc accctgtctg ctcatcaatt ttggcacgca gaccaagcgc acgggcggtg
    21781 gcatatgccg ttgcgtcggc attcagcacc gtgtcccagc cagtaaagtc aggccaaatc
    21841 agcatccctt cacgctgact gaagttttca cggtaagtga tcgcctcctg caccgtcttg
    21901 cagccatacg ctgacaggta agcaaaccca cgcaggcttt gcgccacgcc cagcaactca
    21961 gtagcaacgg ctttattatc gtgccccggc acgccgagga tgcgcggttt aacaccgagc
    22021 tgtgactggg cagataacag ggctttcata ccagtttttt taccttcagc agtcactgcg
    22081 ccgatgatat tggtcgtggt ttcttcttcc gtttcaccct gcggcacacg cacaacaacg
    22141 gtcacgggtt ttgcctggtc agcgatggca tccagcgagc gggccagcgt accggattca
    22201 cccgccttac cgctggcagt aagcacatca gtgatcagca cgggtttatt aagagggaac
    22261 atttttgcat cggcatcatc gcccgtgcag accatgccca cgatggcggt gctcaccgtg
    22321 gtaatggatc gagtgccttc gttgacttca acaacgcgca ctccgtggtg gtaatcctga
    22381 gccatagtgg cgaacctcct gattggatta ggcttcgccc tatgttgaag tgattgtgtc
    22441 tgacaagcag ctaagcgcag ttgtaccgtt attcacacaa aatgacggta tttatctgct
    22501 tgcagggaaa acaggccaaa taatatcagg tgcggtgctg gtatctgttg ccgtcacagc
    22561 ctcgatataa tccagcacaa cgttaagtcg ggtagtttcc tcttgcgtca gtttgcgccc
    22621 ggcctgcagc tttaactgaa tcacgctgat attggccatt gctgcgtcta acagcgactg
    22681 ttttttctgt tcagcgtcag ctaccagttc atcatgagaa cgttcctgag tgggtggtgc
    22741 agtaaatccc ccgtctgaat aaacccagcc gattccgggc tgctcactga tatcagaaat
    22801 atcaatgagc tgctgaacat ccggcactgt gaattcagcc tcgccatccc agacaatgac
    22861 attcacaacc atcccatttt caataactgc atatgacgca ttcattatgc aaactcctcg
    22921 ataatacaaa tccctgcagc gcctttcccg cccgtcatac tggttccgct ataaccggca
    22981 tcgtatgcac caccgccacc tgaaccatat gccttgccgc taacaccacc gccagcgcct
    23041 gcgcgtccac cgccacccca gtacgatgtt ccgccttcac cgctgacgcc gatatttccg
    23101 gactgaccgt cgcctccaca tccaccaatg atgtttatat cgccagtact cggcacacct
    23161 ccgttaccac cgtttgtgtt tgtgactcct gactttccgc cgcctgcgcc accaggagca
    23221 attaacgatg cgaataagct attaccgcca ttgatgccgt acgtcgcact aacaccaccg
    23281 gccccacctg cgccgatagt gactggataa ctattctgtg tcggggtcat tattgaaatt
    23341 attgttccac cggccccacc accagcaccg aaaaacgttt cattattgga tatagccttg
    23401 cagccgcccc ctccgccacc gccgcccgtt attgtgaccc tgatccgttt tgttcctggc
    23461 gtcggggtgt acgtacctga tgaggtgaaa acccgggtat tcagccagcg tccaacgtat
    23521 ccacttgcat cgctcagacc aagatattga ataaccgcct gtgtgcttgc tttatccaga
    23581 atattccgtc caacgttagt cagggttgtc aggctggctg tatcattccc cgtaaaatac
    23641 ggaagtttgt ctgccgcagt ggcaaggcct gccagtgcgg tcagcgtggc gtcggcaggt
    23701 tgttttccat tggcaaggtc atatacagcc ttcacagc
//
LOCUS       DADSZD010000065        23564 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.065, whole genome shotgun sequence.
ACCESSION   DADSZD010000065 DADSZD010000000
VERSION     DADSZD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.065"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            82..777
                     /gene="araD"
                     /locus_tag="J1Z28_002265"
     CDS             82..777
                     /gene="araD"
                     /locus_tag="J1Z28_002265"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA7717095.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGPIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            852..3203
                     /gene="polB"
                     /locus_tag="J1Z28_002266"
     CDS             852..3203
                     /gene="polB"
                     /locus_tag="J1Z28_002266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBA7717096.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDIRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNVWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDTQL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            3368..6274
                     /gene="rapA"
                     /locus_tag="J1Z28_002267"
     CDS             3368..6274
                     /gene="rapA"
                     /locus_tag="J1Z28_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBA7717097.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDETEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            6286..6945
                     /gene="rluA"
                     /locus_tag="J1Z28_002268"
     CDS             6286..6945
                     /gene="rluA"
                     /locus_tag="J1Z28_002268"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBA7717098.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(7063..7878)
                     /gene="djlA"
                     /locus_tag="J1Z28_002269"
     CDS             complement(7063..7878)
                     /gene="djlA"
                     /locus_tag="J1Z28_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBA7717099.1"
                     /translation="MQYWGKIIGVAVALIMGSGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            8133..10487
                     /gene="lptD"
                     /locus_tag="J1Z28_002270"
     CDS             8133..10487
                     /gene="lptD"
                     /locus_tag="J1Z28_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBA7717100.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            10540..11826
                     /gene="surA"
                     /locus_tag="J1Z28_002271"
     CDS             10540..11826
                     /gene="surA"
                     /locus_tag="J1Z28_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBA7717101.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            11826..12815
                     /gene="pdxA"
                     /locus_tag="J1Z28_002272"
     CDS             11826..12815
                     /gene="pdxA"
                     /locus_tag="J1Z28_002272"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBA7717102.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            12812..13633
                     /gene="rsmA"
                     /locus_tag="J1Z28_002273"
     CDS             12812..13633
                     /gene="rsmA"
                     /locus_tag="J1Z28_002273"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBA7717103.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHTTMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            13636..14013
                     /gene="apaG"
                     /locus_tag="J1Z28_002274"
     CDS             13636..14013
                     /gene="apaG"
                     /locus_tag="J1Z28_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBA7717104.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            14020..14868
                     /gene="apaH"
                     /locus_tag="J1Z28_002275"
     CDS             14020..14868
                     /gene="apaH"
                     /locus_tag="J1Z28_002275"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase
                     (symmetrical)"
                     /protein_id="HBA7717105.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLSE
                     GEAVAS"
     gene            complement(14865..15179)
                     /locus_tag="J1Z28_002276"
     CDS             complement(14865..15179)
                     /locus_tag="J1Z28_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
                     /protein_id="HBA7717106.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(15182..15415)
                     /locus_tag="J1Z28_002277"
     CDS             complement(15182..15415)
                     /locus_tag="J1Z28_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBA7717107.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(15501..15980)
                     /gene="folA"
                     /locus_tag="J1Z28_002278"
     CDS             complement(15501..15980)
                     /gene="folA"
                     /locus_tag="J1Z28_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBA7717108.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(16172..18034)
                     /gene="kefC"
                     /locus_tag="J1Z28_002279"
     CDS             complement(16172..18034)
                     /gene="kefC"
                     /locus_tag="J1Z28_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBA7717109.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(18027..18557)
                     /gene="kefF"
                     /locus_tag="J1Z28_002280"
     CDS             complement(18027..18557)
                     /gene="kefF"
                     /locus_tag="J1Z28_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBA7717110.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(18665..19996)
                     /locus_tag="J1Z28_002281"
     CDS             complement(18665..19996)
                     /locus_tag="J1Z28_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7717111.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(20055..20342)
                     /gene="fixX"
                     /locus_tag="J1Z28_002282"
     CDS             complement(20055..20342)
                     /gene="fixX"
                     /locus_tag="J1Z28_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBA7717112.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(20339..21625)
                     /locus_tag="J1Z28_002283"
     CDS             complement(20339..21625)
                     /locus_tag="J1Z28_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBA7717113.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELVVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(21676..22617)
                     /locus_tag="J1Z28_002284"
     CDS             complement(21676..22617)
                     /locus_tag="J1Z28_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBA7717114.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(22632..23402)
                     /locus_tag="J1Z28_002285"
     CDS             complement(22632..23402)
                     /locus_tag="J1Z28_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBA7717115.1"
                     /translation="MKIITCYKCVPDEQDIAVNNDDGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         5699 a         6517 c         6118 g         5230 t
ORIGIN      
        1 cgccgacgcg tcttatcagg cctacacgct gcgattttgt aggccggata agcaaagcgc
       61 atccggcacg aaggagtcaa catgttagaa gatctcaaac gccaggtctt agaagccaac
      121 ctagcgctgc caaaacacaa cctggtcacg ctcacctggg gcaatgtcag cgccgttgat
      181 cgcgggcgcg gcgtcctggt gatcaaacct tccggcgtcg actacagcat catgaccgct
      241 gacgatatgg tcgtggtcag catcgaaacc ggtgaagtgg ttgaaggtac gaaaaagccc
      301 tcctccgaca cgccaactca ccgactgctc tatcaggcat tcccgtctat tggcggcatt
      361 gtgcacacac actcgcgcca cgccaccatc tgggcgcagg cgggccagtc aattccggca
      421 accggtacca cccacgccga ctatttctac ggtcccattc cctgcacccg caaaatgacc
      481 gacgcagaaa tcaacggtga atatgagtgg gaaaccggta acgtcatcgt agaaaccttc
      541 gaaaaacagg gtatcgatgc agcgcaaatg cccggcgtgc tggtccattc tcacggccca
      601 tttgcatggg gaaaaaacgc cgaagatgcg gtgcataacg ccatcgtgct ggaagaagtc
      661 gcttatatgg ggatattctg ccgtcagtta gcgccgcagt taccggatat gcagcaaacg
      721 ctgctggata aacactatct gcgtaagcat ggcgcgaagg catattacgg gcagtaatga
      781 ctgtataaaa ccacagccaa tcaaacgaaa ccaggctata atcaagcctg gttttttatt
      841 ggattttcag cgtggcgcag gcaggtttta tcttaacccg acactggcgg gacaccccgc
      901 aaggaacaga ggtctccttc tggctggcaa cggacaacgg gccgttgcag gttacgcttg
      961 cgccgcaaga gtccgtggca tttattcccg ccgatcaggt tccccgcgct cagcatattt
     1021 tgcagggtga acaaggcttt cgcctgacgc cgctggcgtt aaaagatttt caccgccagc
     1081 cggtatatgg cctttactgt cgcgcccatc gccagttgat gaattacgaa aagcgcctgc
     1141 gtgaaggtgg cgttaccgtc tacgaggccg atgtgcgccc gccagaacgc tatctgatgg
     1201 agcggtttat cacctcaccg gtgtgggtcg agggtgatat acgcaatggc gctatcgtta
     1261 atgcccgtct gaaaccgcat cccgactatc gtccgccgct caagtgggtt tctattgata
     1321 ttgaaaccac ccgccacggt gagctgtact gcatcggcct ggaaggttgc gggcagcgta
     1381 tcgtttatat gctggggcca gagaatggcg acgcctccgc gctcgatttt gaactggaat
     1441 acgtcgccag ccgcccgctg ctactggaaa aacttaacgc ctggtttgcg actcatgatc
     1501 ctgatgtgat catcggttgg aacgtggtgc agttcgatct gcgaatgctg caaaaacatg
     1561 ccgagcgtta ccgtattccg ctgcgtctgg ggcgtgataa cagcgagctg gagtggcgcg
     1621 agcacggctt taaaaacggc gtcttttttg ctcaggccaa agggcgactg attatcgacg
     1681 gtatcgaggc gctgaaatcc gcgttctgga atttctcttc attctcgctg gaaaccgtcg
     1741 ctcaggagtt attaggcgaa ggaaaatcta tcgataaccc gtgggatcga atggacgaaa
     1801 ttgaccgccg tttcgccgaa gataagcctg cgctggcaac ttataacctg aaagattgcg
     1861 agctggtgac gcagatcttc cacaaaactg aaatcatgcc gtttttgctc gaacgggcga
     1921 cggtgaacgg cctgccggta gatcgacacg gcggttcggt ggcagcgttt ggtcatctct
     1981 attttccccg tatgcatcgc gctggttatg tcgcgcctaa tctcggcgaa gtgccgccgc
     2041 acgccagccc aggcggctac gtgatggatt cgcgacctgg gctttatgat tcggtgctgg
     2101 tgctggacta caaaagcctg tacccgtcga tcatccgcac ctttctgatt gatcccgtcg
     2161 ggctggtgga aggcatggcg cagcctgatc cagagcacag taccgaaggt tttctcgatg
     2221 cctggttctc acgggagaaa cattgcctgc cggagattgt gactaacatc tggcacgggc
     2281 gcgatgaagc caaacgtcag ggtaataagc cgctttcgca ggcgctgaag atcattatga
     2341 atgcctttta tggtgtgctc ggcaccaccg cctgccgttt cttcgatcca cgcctggcat
     2401 cgtcgatcac catgcgtggt catcagatca tgcggcaaac caaaacgttg attgaagcgc
     2461 agggctatga cgttatctac ggcgataccg actcaacgtt tgtctggctg aaaggtgcac
     2521 attcggaaga agaagcagcg aaaatcggtc gtgcactggt gcagcacgtt aacgtctggt
     2581 gggcggaaac gctgcaaaaa caacggctga ccagcgcact ggaactggag tatgaaaccc
     2641 atttctgccg ttttctgatg ccaaccattc gcggggccga taccggcagc aaaaagcgct
     2701 acgccggact gattcaggag ggcgacaaac agcggatggt atttaaaggg ctggaaaccg
     2761 tgcgcaccga ctggacgccg ctggcccagc agtttcagca ggagctgtac ctgcgtatct
     2821 tccgcaacga gccatatcag gaatacgtgc gcgaaaccat cgacaagctg atggcgggcg
     2881 aactggacac gcagctggtt taccgtaaac gccttcgccg tccgctgagc gaatatcagc
     2941 gtaatgttcc gcctcatgta cgcgccgctc gccttgccga tgaagaaaac cataagcgcg
     3001 gtcgcccctt gcaatatcag aaccgcggca ccattaagta cgtatggacc accaacggcc
     3061 cggagccgct ggactaccaa cgttcaccac tggattacga acactatctg acccgccagc
     3121 tacaacccgt ggcggaggga atactccctt ttattgagga taattttgct acacttatga
     3181 ccgggcaact tgggctattt tgagcaaaaa aaagagttcg ccagatacca ttttgatgcg
     3241 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
     3301 ccactacctc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
     3361 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
     3421 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
     3481 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
     3541 tgataccatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
     3601 tggcttgctg acctatatcg gtactcgcct tgatactgaa gagtccggcg tagccctgcg
     3661 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
     3721 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
     3781 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcacca
     3841 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
     3901 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
     3961 cgctgctgaa cgtgtgctga ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
     4021 aatgctgcgc cgtttcaacc tgcgctttgc tctgtttgat gatgagcgtt atgccgaagc
     4081 tcagcacgat gcttacaacc cgttcgatac cgaacagctg gtgatttgct cgctggattt
     4141 tgcccgtcgt agcaaacagc gtctggaaca tctctgtgaa gccgagtggg atctactggt
     4201 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccgagccgtg aatatcaggc
     4261 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcaa ccccggaaca
     4321 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
     4381 ttttgcgcaa ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
     4441 gctgctggct ggtaacaaac tcagcaatga cgaactgaac atgctcggtg agatgatcgg
     4501 cgagcaggat atcgagccgc tgttgcaagc agcaaacagc gacagcgaag atgcccagag
     4561 cgcccgtcag gaactggttt cgatgctgat ggatcgccac ggtaccagcc gcgtgctgtt
     4621 ccgtaacacc cgtaacggtg tgaaaggctt cccgaaacgc gagctgcata ctattaagct
     4681 gccactgccg acgcagtatc agaccgctat taaagtctcc ggcattatgg gcgcacgtaa
     4741 aagtgcggaa gatcgcgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
     4801 aggcgataac gccacctggt ggaacttcga tccgcgcgtt gaatggctga tgggctacct
     4861 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagcggcca ctgcgctgca
     4921 actggagcag gtactgcgcg aacgtgaagg tattcgcgcc gcagtgttcc acgaaggcat
     4981 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
     5041 ggtactgctg tgctcggaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
     5101 ggtgatgttt gacctgccat tcaacccgga tctgctggag cagcgtattg gtcgtctgga
     5161 tcgtatcggt caggcgcacg atattcagat ccatgtgcct tatctggaaa aaacggctca
     5221 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca ttcgagcaca cctgcccgac
     5281 cggacgcact atctacgata gcgtgtacaa cgatttgatt aactatctgg cctcgccgga
     5341 tgagaccgaa ggctttgacg atctgatcaa aaactgccgc gagcagcatg aagcgctgaa
     5401 agcacaactg gaacaggggc gtgaccgcct gttggagatc cactccaacg gtggcgaaaa
     5461 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
     5521 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
     5581 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
     5641 catcaccatc accttcgacc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
     5701 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg ataccggtag
     5761 cagcacgatt tcactgttga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
     5821 gatttatgtg gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
     5881 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
     5941 tgaaaccttt aaccgccagc ttaacgcggt taaccgtcac accggcagca agctggttaa
     6001 cgcggtgcag caggatgttc acgctatcct tcagctgggg gaagcgcaga tcgagaaatc
     6061 tgcccgtgca ttgattgatg cagcgcgtaa cgaagccgac gaaaaactgt ctgccgagct
     6121 gtctcgtctg gaagcgctgc gtgcagttaa cccgaacatt cgtgacgacg aactgaccgc
     6181 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
     6241 tgccctgcgt ttgatcgttg tgacgcatca gtaacggagc cgaagatggg gatggaaaac
     6301 tacaatccgc cgcaggaacc ctggctggtt gtcctgtatc aggatgacca tattatggtg
     6361 gtcaacaagc cgagcggttt gttgtctgtg ccgggtcgtc tggaagagca caaagacagc
     6421 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
     6481 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
     6541 cagttccgcg agcgcgagcc gaaaaaacag tatgtggccc gcgtctgggg gcatccatcc
     6601 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
     6661 aaagtctgtt acgaaacagg taaacctgcg cagacggagt atgaagtggt ggaatatgcg
     6721 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
     6781 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
     6841 gcgagagcaa tggcaccgcg tttgttgcta catgcagaga tgctgacgat tacccatccg
     6901 gcgtatggca atagtatgac gtttaaagca ccagcggatt tttaatagtc acgctggcga
     6961 agttcggtag gccggagaag gcgttcacgc cgcatccggc aattttgcgc cgattgcctg
     7021 atgcgacgcg ggccgcgtct tatcaggcct acacgttgaa acttatttaa accctttctg
     7081 ctgctttatc agctcatagg cctgctgaat ttcctgcgct ttctgcttcg ccatctccat
     7141 catctctggc ggcaaacctt tcgccaccaa cttatcggga tggtgttcac tcatcagctt
     7201 acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca gcacattaca
     7261 ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc cagattgctg
     7321 ctgataaccg ccaccaaact gtgcaccgcc ctgcatcatg cgcaaaaact ggtcaaactg
     7381 agcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt cattcgggtg
     7441 cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac gaattaagtc
     7501 aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg ggtaattgtc
     7561 tgattttccc acccggaacg cattttgcgc cgcagtacgg gaagcgccat gaaggttcat
     7621 tcggtccatc aactggctgg cgatatgaat atcagcttcc gtgacgcgac ctttggattt
     7681 ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac gctcacgctg
     7741 gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc caattaacag
     7801 gcccaatact acgccccaaa agccgctgcc cattattaag gccacggcca cgccaatgat
     7861 ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg ctatctccca
     7921 caatataaag gcgcttttac ccatttctgg ctgcggtcta ttgtgagaca tcgcctataa
     7981 tttgcattat catacctgtc attcaatgcc gtgcctaaca ccacagacgc tattcggaca
     8041 ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg tcacgcgcaa
     8101 cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc actctcctgg
     8161 ccaccatgat tgccactgcc ctttatagtc aacagggact ggcagccgac ctcgcctcac
     8221 agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat accaatgact
     8281 tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac gccgtgttta
     8341 ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa gtgcagctcc
     8401 atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg ctcggtaatg
     8461 tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg aatctgaaca
     8521 ccaaagatac caacgtctgg gagggtgatt accagatggt gggtcgccag ggtcgcggta
     8581 aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat aacggtagct
     8641 ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa attattcatg
     8701 accgcgaaga acaagttgcg gagatctgga acgcccgctt taaggtgggt ccggtaccga
     8761 tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct ggtttcttga
     8821 tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca tattactgga
     8881 acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt cgtggcaata
     8941 tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg atggaactgg
     9001 actatctgcc ttcagataaa gtctataaag atgaacaccc gaacgatgac agttcacgtc
     9061 gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt ttcaacgtcg
     9121 actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag tacggttcca
     9181 gtaccgacgg ctatgcaacg caaaaattca gcgttggcta tgcggtgcaa aacttcaatg
     9241 ccaccgtttc aaccaagcag ttccaggtct tcagcgagca gaacaccagt agctactcgg
     9301 cagaaccgca gttagacgtt aactactatc aaaacgatgt cggtcctttc gatacgcgaa
     9361 tttacggcca ggcggtgcac tttgttaaca ccagagatga catgccagag gcgactcgtg
     9421 ttcacctgga accgaccatc aatctgccgc tctctaataa ctggggcagc atcaatactg
     9481 aagcgaagtt gctggcaacc cattatcagc aaaccaatct tgactggtat aactccagaa
     9541 acacgaccag gctggcagag tccgctaacc gtgtaatgcc gcagttcaag gtagacggta
     9601 gaatggtctt cgagcgtgat atggaaatgc tggcgccggg ctatacccag acactggaac
     9661 cacgcgcgca gtatctgtat gtgccatatc gcgatcagag caaaatctat aactacgact
     9721 cttcgctgct gcaatctgac tactccggcc tgttccggga ccggacttac ggcggtcttg
     9781 accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata tatgatgatg
     9841 ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg gagtctcgca
     9901 ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg gtatgggcag
     9961 gcgatactta ctggcgcatc tctgatcgtt ggggattgcg tggcgggatt cagtacgata
    10021 cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg gatgaagacc
    10081 gtctggtaca gctgaattac cgttacgcca gcccggaata tattcaggca acgctgccta
    10141 agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt gccgtcgcca
    10201 gctggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac accaatgcga
    10261 acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat gcgattcgcg
    10321 tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg gtatatgaca
    10381 acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt ctgggtacgc
    10441 aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt gttgatttac
    10501 cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg gaaaacgctg
    10561 cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt agtcgataaa
    10621 gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg attaatgcag
    10681 tcggtaaaac tgaacgctgc tcaggcaagg caacaacttc ctgatgacgc aacgctgcgc
    10741 caccaaatca tggagcgttt gatcatggat caaatcatcc tgcagatggg gcagaaaatg
    10801 ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc gaaacagaac
    10861 aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa ctacaacacc
    10921 tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa cgaggtgcgt
    10981 cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt gggtaaccaa
    11041 aacgacgcca gcactgagct gaacctgagc cacatcctga tcccactgcc ggaaaacccg
    11101 acctctgatc aggtgaacga agcggaaagc caggcgcgcg cgattgtcga tcaggcgcgt
    11161 aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca ggcgctgaac
    11221 ggcggacaga tgggctgggg ccgtattcag gagttgccgg ggatcttcgc ccaggcatta
    11281 agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg cttccatatt
    11341 ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgactga agttcatgct
    11401 cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg cgtgaaactg
    11461 gaacagattg ctgccgatat caagagtggt aaaacgactt ttgctgccgc agcgaaagag
    11521 ttctctcagg atccaggctc tgctaaccag ggcggtgatc tcggctgggc tacaccagat
    11581 attttcgatc cggccttccg tgacgccctg acccgcctga acaaaggtca aatgagtgca
    11641 ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg taatgtcgat
    11701 aaaaccgacg cggcacagaa agatcgtgca taccgcatgc tgatgaaccg taagttctcg
    11761 gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa aatcctgagc
    11821 aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc gggattggcc
    11881 cggacttaat tgtccagctt gcacagcgtg agtggccagt cgaactggtt gtttgtgccg
    11941 atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc ctccgccctt
    12001 attcccccaa ctcccctgca caaccgcaaa ctgcgggcac attaacgcta cttcctgtcg
    12061 cgctacgtga atctgtcact gtggggcagt tagcagttga aaatgggcac tatgtggtgg
    12121 aaacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg ctgatcacag
    12181 gccccgtgca taaaggcgtc attaacgacg caggcattcc gtttaccggg cataccgagt
    12241 ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc gaagaacttc
    12301 gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct atcacccctg
    12361 cccttttgca cgaagtgatt gctattttgc atcacgattt gcggaccaaa tttggtattg
    12421 ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc ggtcatatgg
    12481 gtacggaaga gatagacacc attattccgg tactcaacga gctgcgggaa caggggatga
    12541 aactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc gataacgccg
    12601 acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac cagggcttcg
    12661 ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg gaccacggca
    12721 ccgcgcttga actagcggga cgtggtaaag ccgatgtcgg cagttttatt acggcgctta
    12781 atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc agggccactt
    12841 agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg acagcattgt
    12901 ttctgccatt aacccgcaga agggccaggc gatggtcgaa atcggccccg gtctggcggc
    12961 attgaccgaa ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac ttgaccgcga
    13021 tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga tttatcagca
    13081 ggatgcgatg acctttaact ttggcgaact ggccgcgaaa atgggccagc cgctgcgtgt
    13141 tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt ttagctatac
    13201 tgatgccatt gccgacatgc actttatgtt gcaaaaagaa gtggtgaatc gtctggttgc
    13261 aggaccgaac agcaaggcgt atggtcgatt aagcgtcatg gcgcaatact attgcaacgt
    13321 gatcccggtg ctggaagtac cgccgtcagc ctttacacca ccacccaaag tggattccgc
    13381 cgtcgtgcgc ctggttcctc atacaacgat gcctcacccg gttaaagatg ttcgcgtgtt
    13441 gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta acagcctcgg
    13501 caacctgttt agcgtcgagg tgttaacggg aatggggatc gacccggcga tgcgagcgga
    13561 aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga acgcgccttt
    13621 gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca aagcgtctac
    13681 attgaggccc aatcttcacc tgataatgaa cgttacgttt ttgcttatac cgtaaccata
    13741 cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat caccaatggc
    13801 aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc acttatcgcg
    13861 cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct gggcaccatg
    13921 cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga cattcccgta
    13981 ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata ccttattggc
    14041 gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga atttacccct
    14101 gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcggcccggg ttcgctggat
    14161 gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg taatcacgat
    14221 ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga tcgcctgaca
    14281 ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg ccagcctttg
    14341 ctgcaaatcg acgaagagaa aaagttggtg atggcccacg ccgggatcac gccgcagtgg
    14401 gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc gagtgactcc
    14461 tatcccttct tccttgatgc catgtacggc gatatgccaa ataactggtc accggaattg
    14521 cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg tttttgcttc
    14581 ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaggcccc tgccccactg
    14641 aaaccgtggt ttgcgattcc tggccccgtt gctgaagagt acaacatcgc ctttggtcac
    14701 tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga taccggctgc
    14761 tgctggggtg gtacattaac ctgcctgcgc tgggaagata aacagtattt tgtccagccg
    14821 tcgaaccggc ataaggattt gagtgaggga gaggcggtag cgtcttaaat cccgtcgagc
    14881 ataaaatcta atgctccttt aatcaacgtt cggtgcgcgc tggcatcgca aaatttcgtt
    14941 cccagcgcgt taactggaac agttgcagtc tcatgcgtca tgagcacata ttctttgccg
    15001 tctaccagta aaaggattgg gttaagccgt tctggtgggc gaatacggct aaatcgttca
    15061 ataggtgtta atggaataac gatacggcga ttaatctcac caataatgtc gctggtaaca
    15121 tcaataacaa agggaaacgc ggcgttcctg ccgcgactgc ggtataccgt aaattgcatc
    15181 gctagaagct ccggtactca tcagagaaac atccggtttc atcagccaat tgatttaacg
    15241 cagcgatagc ttctgcgttc tcttcacgcc aacgtgttgc tgcatgtttt ttaagttcag
    15301 cattgagcgc aaccgtaagg gtggcgctca tatttaagcc tgcatcacga gcgcgattga
    15361 ctaactcacg gtcgacggtg acggtcacac tttgtgtggt acgttttgca gtcatgatct
    15421 cgtgctcctg tacaatttat acgtctagta tacacggata tctgcacgta tcagtgatgc
    15481 cggaattcta atatacaaaa ttaccgccgc tccagaatct caaagcaata gctatgagag
    15541 ttctgcgcat cggcgtcgtg gaattcactg aataccgatt cccagtcatc cggctcgtaa
    15601 tccgggaaat gggtgtcgcc ttccacttcc gcgtcgatat gcgtcagata cagtttttgc
    15661 gctttcggca ggaactgctc ataaacgcga ccgccgccaa tcaccatgat ttctggtacg
    15721 tcaccacacg ccgcaatggc ttcatccacc gacttcaccc acgttacgcg atcgtccgta
    15781 cccggttgac tgctgaggat aatatttttg cgtcctggca acggacgacc gatggattcc
    15841 caggtatggc ggcccataat cacgggttta tttaaagtgt tgcgtttaaa ccaggcgaga
    15901 tcggcaggca ggttccacgg catggcgttt tccatgccga taacgcgatc taccgctaac
    15961 gccgcaatca gactgatcat tgagatttcc cgataaaaaa aattgtcgcc actatacgta
    16021 aagcgtaaac cgtcgtcgac tggtgcgagg atgatgttga ggaaaatttt atattctgct
    16081 ggcgagtcca cgctctctcc ctggactcgc cgcattacaa tgaaacaaaa acaaacagtt
    16141 agctgtaaag cgtgatttac gtcactcttt attaagatga gggcttcgtt tccggttcat
    16201 ccgccatatt gccagtatgt ttcccttctt cggttccctg ccagccatgt cgttgaatta
    16261 atgacagatg ttcacggtct tcggtaatga tctcacttaa catcgcgctg gtgcgtttat
    16321 agaccgccgc acgggctttg gtgtcgttct caaccattgc catctcttcc accatctgaa
    16381 tattaaagcg gcggaacaca tcggcgcgtt ctcgcgcttc atacggcccc agacctaaac
    16441 tttccagtgc cagacgcccg gttttcagcg caccttcgaa ggtttcacgc tccggctttt
    16501 caacgcctgc ctgacgcaaa cgaatgtagt ggtcgacatc gcgggcgcgg gcaataatct
    16561 gcaaatgtgg gaaatgttct ttcaccatct ccgtcagttg caggttggtt tgcggatcgt
    16621 cgatggcgtt aatcagcact tccgctttcg ccgctccggc agattccagt aaatccatcc
    16681 gcgtggcatc gccataaaac actttcatac cgaatttacg cagcgtttcg atatggtccg
    16741 ggtcgtgatc gagcaccacc attttcaccc cgctggagag cagtaaacgt ccggttatct
    16801 gcccaaaacg accgaatccg gcaatgatta cccgcggctg ttcttcgtcg atctcatcgg
    16861 cttcacgcgc ctcttcagta gaagattgct caaggcgatt gaggatcacc agcagaatcg
    16921 gcgttgccgc catcgacagt gccaccgcca gagtcagcga tttcgcccac tctggctcca
    16981 gcacattcgc catctgcgcc gcaccaaata ctacaaaggc aaactcactg ccctgtccta
    17041 acagcaccgc aaaccagcga cgctgtttat ttggcacctg caacggtcgg gcaatcagcc
    17101 acagcatggc gattttgatg atgaggaaac cgagcagcaa aatgacaatg cgcaacggat
    17161 tttcaagcag cgtgccaaag tctatcgaca tgccaacgcc gatgaaaaac agccccagca
    17221 gcaaaccttt aaatggttcg atatcgcttt ccagcgcatg acggtattcc gagcttgcca
    17281 gcagtacgcc cgccagaaac gcgcccatcg ccatcgacaa gccgacctct tccagcagca
    17341 aaccaaagcc aaacacgagg aataacgcca cggcactaaa cacttcccgc aagccagagc
    17401 gggcaacaaa acgcagtgcc ggacgcgtga catagcgccc cagcaatacc accagcacca
    17461 gcgcgcccgc cacttttaac gccgagagaa caaatgcgcc catcgtcgtc gaggcactgc
    17521 tcgctgccag tagcgggatc atcgccacca gtgggatcgc cgcgatatcc tggaacagca
    17581 gcaccgcaaa ggcactgcgg cccatttgcg tcaccatcag attgcgttca ttcatcgcct
    17641 gcatggcaat cgccgttgat gagagcgcca gcgtcatgcc gatcaattcc gcgacctgcc
    17701 agcgcagccc aagtaacata caaaacagcc ccagcaggcc gccgcaaatc accatctgca
    17761 atgcgccacc accgaacacc gccgcacgca gtttccacag cctttgtgga tcgagttcga
    17821 ggccgataat aaacagcatc agcaccacgc caatctcggc aaagtgcaga atagattcgg
    17881 catcggtcac cagtcgcagc ccccacgggc caataatgca gcctgctatc aggtagccaa
    17941 gaaccgatcc cagaccgaga cgtaccgcaa tgggaacaat cagcgccgcc gaaccgagat
    18001 aaatcagcgc ctggataagc gtatggctat ccatgatgtg cctcctgcca ttccagcaga
    18061 cgttgcttat agtgccgcgc ctgcccttcg agggtttcgt cgtcacaaat aaaggtgcag
    18121 tgcatggcaa acggtggcag ccagttcagc ccgcagtaga ttgccgtcgc ctgtagcggc
    18181 tgcgacagca catcaaagcc cggatgcgca ccaatctcaa aatggctttc cccaccgccg
    18241 gtagtcaccg cccacagcaa atgtttgccg tgcagcgccg tgccgccgtg accgtaagcc
    18301 cagccgtgcg agaaaacttt atcgatccaa agtttaagaa gcggtggaat gctgtaccac
    18361 tgcattgggt gctgccagac gatcagatcg gcacgagaca gcgcctcctg ttcggcggca
    18421 atgtcgatat tgaagtcagg atagagttga taaagagagc gaatttcgac gccttccagc
    18481 gtccttgcct gttcaagcat ccgtttattc gcatgggaat gatgcggata tggatgcgca
    18541 taaattataa gaatcatagg tagcccgcgt gattacgcct ttgttttccc acaaagtgta
    18601 gtcatatatt cacgcagctt atagtcaata atattgattc agaaaagaga gaaatctgaa
    18661 caaattaccc cattctctgc ccgcggatcg tcatattgct ggtctgcgcc agtgacatcc
    18721 ctcgagtctc cggggcaaac gctacggaaa tcaacaagcc aaatagcgag atacccgccc
    18781 ccattagcat cgtgttactg ataccgtaat tattgataaa gatcggcagt gcccaggtcg
    18841 atacaatagt gccaatacgg cttaaggaca taatcacgcc cacggcagag gcgcggatat
    18901 ccgtcgggaa gagttcatta ggatagagcc actgcaaatt acccggcccg ccagagaaaa
    18961 aggcatacac cgcaaaggcc atcactacca gccagatccc catatccggg atcagcccca
    19021 aaaccgccag cgccagcgtc atcatggcaa agctgccaat caacaatgga cgccgtccgg
    19081 cagtgtttag ccacagcatc ggcggaatac agccgagcat aaagaacagg ctaatcacca
    19141 cgttccccag tgccgcgttt ttgccaaccc ccaatcccaa caaaccaacg atttgcgggc
    19201 caaaggtgta aatggcaaac atggggatca cctggcaggt ccagatggcg gcaacaaaca
    19261 gaacaaaagg aaaatggcgg cgattaaaca gatcgcgaaa acgggtttgc tgcggctgct
    19321 cttcctcgaa agccaccggt tcgccaaaca gttttatcat catctcttcg cactctttta
    19381 ctcgcccttt gcgtaacaac cagcggggag attcaggcag ttcgaatcga ccaatcaaaa
    19441 tcaacaaaca gggaatcgct gcgctaccca gcatccagcg ccagccgcct tccacatcat
    19501 aaagccaata gccgaccaaa tcggcgcagg tcgcgccgac ataccacatc gcggcgataa
    19561 agctgatgga aaacgcccgc tgacgggtac tggagaactc ggtgatcatt gaggtggcga
    19621 taggataatc cgcaccgata acaatgccga taagtacccg catcaccaac agttcgacgg
    19681 gggatgagac aaacatcgtc gccaccgata tcacgccgat ggcgatgata tcaatgagga
    19741 acattttgcg ccgtccgact ttatcggaga tatagccaaa cagcgatgtg ccaacgaaca
    19801 gcccggcgag cgttcccgcg cccagcaagc caatccagtc agcgtccagt ttcagcgccg
    19861 gagtcagttg ctccagcgcc acgccaatca ttaccagtac ataaccatcc agaaacggac
    19921 caccgcttcc ccacagcaaa atgcggcggt gaatagagga gaatttaaga tcatcaaagt
    19981 ttctggacgg ttgcatgggt tattcctgtt tttttgcaat gagagtcagt acggcattac
    20041 gtatcaaacc aacatcagcc gtaacggaac tccacaccaa aggtgccgcg cgggtattcc
    20101 cactgttcca gcgccgaccc cagccccaga atgcgacagg tgccgcactc cagacatccg
    20161 gcgtaatcga agcgcacact gccgtcatcc tgcttcttgt acagacctgc ggggcacgct
    20221 ttcaccagca gctccagggc ctgtttatct gcatcggcct tcacaacaat gtgcggatgc
    20281 tcttcatcga cattgaattt attgacgccc agtttgacgt ccacattgac gggagaagtc
    20341 ataaaacggt cactcctttc atgccatcct tgatcagatt gatgaagccc actttcttgc
    20401 cgtggcggag gattttcttg cgcatcagtt ccggcgcact gccgtcaatg gtgaacaggt
    20461 cacgcgccac gcccaccacc agttccgggt agccgctaaa catgcgtggg ttatcaagga
    20521 acgccggtag tttctggtac atgcgcatat cgcgcagcgg gccactctca agatgttgac
    20581 gatattccgc gagtttttgc ttactgaaat cgtcgctttt catcgctgaa agcacagttt
    20641 ttgctgctgc ttccccggcg gcaatcgcca gatccatacc gcgaatggta aaaccgaggt
    20701 tcatacacat tccggcggca tcaccggcaa tcagtacgcc gtcaccaacc agctccggca
    20761 gcatgttgat gcctgcttcc ggcactacgt gagcggaata ttccaccagc ttgccgcccg
    20821 cgatcagcgg tgcaacggcc ggatgttgtt tgaaatcttc cagcatttgc ggcaccgatt
    20881 ttttcgcgtc atgcagatga tggagaccac aaaccagccc cagcgacagg gtattttcat
    20941 tggtataaag gaagccgccg cccatcaggc catcagtagg tgatcccgca aacaggcaag
    21001 ccgcgccctg attaccctgc aactgaaaac ggtcttcgat aaccgacttc ggtaactcga
    21061 tcagttcctt cacgccaacc gccacatccg tcggtttaac acgttttgcc atccccagct
    21121 tttcggcaag gatggaattc accccatcag caaggatcac cgttttcgct tcaatcacat
    21181 cgccatcggc ttctacaccg acgactttgc catcgcgctg tacgaggtta tcgacgcgga
    21241 tcccggtaat taactgcgcg cccgcttctt cagcctgctc catcagccag gcatcaaatt
    21301 tactgcgcaa aacggagtaa gaacgctggg atggcgaggt ttcgtcgcca ttgcagtagt
    21361 ccatagtcat cgctgacttc tccgtcataa acgcaagttt ttcatgggtg atcaggcgtt
    21421 ctacgggggc ggagtcagcg aaaccaggaa taatgtgttc cagactgtgg gcatagagac
    21481 gcccgccggt gacgttcttg gcaccagcgg aattgcctcg ctcgataact aacacttgcg
    21541 caccttcgcg ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga
    21601 tggcgtcaaa gatatcttcg gacataagag ctccctgtca gcggcagggc caaaatgcag
    21661 ccctgccaga gtggatcaac gcgctaaagc tgcggtcagt gccggaagga tcttcacggc
    21721 gtcgccaaca atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttgttgat
    21781 ggcgaaaatg gtttgcgacg cattagcgcc aaccatgtgc tggatctgcc cggagatccc
    21841 caccgccagg tacagttcag gtttcagcat caggttggag ataccgacat agcgttcgtg
    21901 ctccatccat ttttcgtttt ccgccaccgg acgagaacag gccaactccg cacctatcgc
    21961 cttgcaaagc tgttctgcca gcgcaatgtt ctctttgctg ccaataccgc gaccaacgct
    22021 gaccaccagg cgggctttgt cgagatcgac gctgttgctc tgacgcgcct gggttgccgt
    22081 gcgggtaatc gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga
    22141 cgcgtctggc tgagccacat cgaacgtgcc gctgctgatg gtcagaaccg catacggcgt
    22201 ggcaatgcgt tcttcgccaa tcgccagacc gccgtaaacc atgtgtttca ctgtcgcttt
    22261 accgtcctgc acgctaacag cgctggcatc gttagacacc gccgcgttaa ggcggtaacc
    22321 cagtttcgcc gccagtaatt tgccgcgacg ggtgtttggc aacagcacca gaccgtctgc
    22381 gccgtgctgg cgaatagtgt cagccatgac accggcgtaa tcttcgatca tccgctcgtc
    22441 cggtttgccg cttaatttcc agacatgatt agcgccgagc tggattgcct gtgcgccgtc
    22501 ggcatcattg aggacgaacg tgttgatttg attagctaaa gcctgcgcac cgttcatcag
    22561 ttccggcaga cgagaagggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc
    22621 atcccctgta attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg
    22681 ccgtcgcctt caatcacgat gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt
    22741 tctgaccagg ctgcctctgc gttaaaaccg atgtccgccg ccgaccatac ctggactggc
    22801 tttttcgccg cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg
    22861 gaaacagcaa caaccgcagg cagcggaatg cttaaggttt ctgtttcatc ttccagttcg
    22921 cgctcaacgg tgagggtatc tgcagtcagg gagataattt tgctgacgcc gttaactgcc
    22981 ggaatattaa ggatttcgcc taccagcaaa ccaacctgct gggcataaag gtcggaagaa
    23041 ccatcgccac agaggatcaa atcaaagcct gctttctgag cggctgcagc cagtacgctc
    23101 gccgtttgtt gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc
    23161 ggaccgcgcg atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca
    23221 cttaaggccg tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata
    23281 gcgttgagat cgtattggct tattttggca tcggctttgc tgaagtctaa cgaaccatca
    23341 tcattattga ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc
    23401 attgcatctc cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt aattacagat
    23461 atcacgccct ttaatttgga aataaaactt taatcaccaa tattgaaaat gtcacacgca
    23521 gataaatcca actttcaata ttgttaagct cctcaccaat attg
//
LOCUS       DADSZD010000066        23545 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.066, whole genome shotgun sequence.
ACCESSION   DADSZD010000066 DADSZD010000000
VERSION     DADSZD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.066"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            102..1040
                     /locus_tag="J1Z28_002286"
     CDS             102..1040
                     /locus_tag="J1Z28_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003036294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7717116.1"
                     /translation="MSHEHAVSFRHNNWQVAATLRLPEGFDDRHKYPAIVCAHPISSC
                     KEQTSGAVYGRKLTEAGFITLAFDASTQGESSGEPRFSENPSTRVEDFSCAADYLMTL
                     PFVDEERIGVLGVCGGGGYAISAATIDHRFKAIATVVAANYGRIMREGDGSPDAALRT
                     IEALGKQRTAEARGAEPLIVGYIPASAEELAHTGIDDIDMTEAVDYYRTPRGQQPGSP
                     NKLRFTSVAQSLSWDAFAFTEKLLVQPLHIVIGDVPGGFGSYRDGYELYQRARSAEKT
                     LQIVKGVSHYDLYDRPEATSQALAGIIPFFNKHLSG"
     gene            complement(1185..1466)
                     /locus_tag="J1Z28_002287"
     CDS             complement(1185..1466)
                     /locus_tag="J1Z28_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717117.1"
                     /translation="MNYDEITKITAERISDYMAEAVNTDSIAVAEMFHNAAWGVRTLW
                     FELVTKIDIDIHKKNRYASYDLRRKIEMQHEEFQKMTEREQVPLLKSPE"
     gene            complement(1572..1847)
                     /locus_tag="J1Z28_002288"
     CDS             complement(1572..1847)
                     /locus_tag="J1Z28_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7717118.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(1847..2131)
                     /locus_tag="J1Z28_002289"
     CDS             complement(1847..2131)
                     /locus_tag="J1Z28_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBA7717119.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSRHVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(3049..4071)
                     /locus_tag="J1Z28_002290"
     CDS             complement(3049..4071)
                     /locus_tag="J1Z28_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBA7717120.1"
                     /translation="MVIKNTRPGHWAQLPPEEQIRFWEDYEAGRATSFLVEPERKRTK
                     RRRGEHSTKPKCENPTWYRPARYKALSGQLGYAYNRLVKKDPVTGEQSLRMRMSRHPF
                     YVQKRTFAGRKYAFRPEKQHLLDAIWPVLISFSDAGTHTVGMSVSRLAREISPKDSKG
                     KVIPELEVTVPRLSRLLAEQVRFGVLGVSEETMWDRENRQRLPRYVWITPAGWQMLGV
                     DMVKLHEQQQKRLCESEIRQQLIREGVLREDEDISVHAARKRWYLQRSLDALKHRRAK
                     AAASKRARGLKKLPADQQIYEMSQHILKRMPPDEAYWCTPERLQQLAIRELYQLELTL
                     AAPPPH"
     gene            complement(4061..4594)
                     /locus_tag="J1Z28_002291"
     CDS             complement(4061..4594)
                     /locus_tag="J1Z28_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBA7717121.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCEKLMGKAVGFTET
                     FEFDIASACARANGKRKRKPSVLRRRAIDALLQGMCFYYDPVSNQVHRSITELALDCG
                     LARKNAHGHLAIERAVRAIKSLEEDFGFIVCSPSSDFYNKRCTITLTPRFFEFLGIFP
                     LALTEARLAVLRSGYGD"
     gene            complement(4587..4661)
                     /locus_tag="J1Z28_002292"
     CDS             complement(4587..4661)
                     /locus_tag="J1Z28_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBA7717122.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAVWCD"
     gene            complement(4894..5154)
                     /locus_tag="J1Z28_002293"
     CDS             complement(4894..5154)
                     /locus_tag="J1Z28_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBA7717123.1"
                     /translation="MSQIENAVTSSPKRIYRKGNPLTGAEKQRISVSRKKGTHKAINV
                     FIQSELKDDLIQLCKDSGLTQKEMIEHWILKEKAAMDDVNRR"
     gene            complement(5394..5984)
                     /locus_tag="J1Z28_002294"
     CDS             complement(5394..5984)
                     /locus_tag="J1Z28_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBA7717124.1"
                     /translation="MKLIIFIRIVLIIAALLIRIILRSVNQYSPLLMQLHAAGIRTGN
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWRLFRMVSQWHVDVVIVERRSFSIVAAVELDDAGHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(6022..6231)
                     /gene="hha"
                     /locus_tag="J1Z28_002295"
     CDS             complement(6022..6231)
                     /gene="hha"
                     /locus_tag="J1Z28_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBA7717125.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(6298..6738)
                     /locus_tag="J1Z28_002296"
                     /pseudo
     CDS             complement(6298..6738)
                     /locus_tag="J1Z28_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            complement(6984..7196)
                     /locus_tag="J1Z28_002297"
     CDS             complement(6984..7196)
                     /locus_tag="J1Z28_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBA7717126.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(7331..7891)
                     /locus_tag="J1Z28_002298"
     CDS             complement(7331..7891)
                     /locus_tag="J1Z28_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBA7717127.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYETEGYVTEHISQEEES
                     YAAERLDKICRQNRIKAELQAVLDEK"
     gene            complement(7946..8692)
                     /gene="traX"
                     /locus_tag="J1Z28_002299"
     CDS             complement(7946..8692)
                     /gene="traX"
                     /locus_tag="J1Z28_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     acetylase TraX"
                     /protein_id="HBA7717128.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILMMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLALVACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            complement(8712..13982)
                     /gene="traI"
                     /locus_tag="J1Z28_002300"
     CDS             complement(8712..13982)
                     /gene="traI"
                     /locus_tag="J1Z28_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBA7717129.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAE
                     WMQTLKETGLDIRAYRDAADQRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMTFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMDQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGYSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKTRAGETPLETAISLQ
                     KAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTSFIDLGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEMLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMQKGEAFPDV
                     PMTLYEAIVRDYTCRTPEAREQTLIVTHLNEDRRVLNSLIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNQDALALVDNVYHRIVGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAERLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTTEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREITGQERDRAAITEREAALPESVLREPQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLSGD"
     gene            complement(13982..16162)
                     /gene="traD"
                     /locus_tag="J1Z28_002301"
     CDS             complement(13982..16162)
                     /gene="traD"
                     /locus_tag="J1Z28_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBA7717130.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHEKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGCQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGEPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQTRP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMVSLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEIEQQLPPGISESGEVV
                     DMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(16415..17146)
                     /gene="traT"
                     /locus_tag="J1Z28_002302"
     CDS             complement(16415..17146)
                     /gene="traT"
                     /locus_tag="J1Z28_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBA7717131.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(17178..17675)
                     /locus_tag="J1Z28_002303"
     CDS             complement(17178..17675)
                     /locus_tag="J1Z28_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein"
                     /protein_id="HBA7717132.1"
                     /translation="MIMRCLAHIALVTVIQFIACYLADWGNAETVFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIISTLYELRRVSGCI
                     SIKKWGQNQLKDQYDGSEKLDFGGIEQNPTIYYNPSTGYPMHGGFDSAGNTFGTRWQD
                     YYDRQ"
     gene            complement(17691..20513)
                     /gene="traG"
                     /locus_tag="J1Z28_002304"
     CDS             complement(17691..20513)
                     /gene="traG"
                     /locus_tag="J1Z28_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBA7717133.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGAKLVSKSTDFLSRNPEIANLFQDY
                     VQHCVMGDIYLNHKYTLEELMVSADPYTLIFFRPSPLRGVYDSNNNFVTCKDASVSLK
                     DKLNLDTQSGGKTWHYYAQQLFGGRPDPNLLFSTLIGDSYSYFYGSSKSASQIIRQNV
                     TINALKEGITSYAARNGDSASLVNLATTSSMEKQRLAHVSIGHVAMRTLPMTQTILTG
                     IAIGIFPLLVLAAVFNKLTLSVLKGYVFALMWLQSWPMLYAILNSAMTFYAKQNGAPV
                     VLSEISQIQLKYSDLASTAGYLSMMIPPLSWMMVRGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWSDDDAHQASSGSRASYDARHDIDAKA
                     SKDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQIQPSVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSIRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(20510..21883)
                     /locus_tag="J1Z28_002305"
     CDS             complement(20510..21883)
                     /locus_tag="J1Z28_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBA7717134.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFVQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEIIPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(21883..22296)
                     /gene="trbF"
                     /locus_tag="J1Z28_002306"
     CDS             complement(21883..22296)
                     /gene="trbF"
                     /locus_tag="J1Z28_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBA7717135.1"
                     /translation="MNHRKYQRELIMRENKNNPELKIRSTERDYKYISRITDRYAGLS
                     LVFLTAGIVLWTVMNIIFDACIDSWKADPELNNSTYMWNILMYAIPYTLYALADGFLV
                     TFFSVPNVRINIRKYRDIPVETPYAPGEQIKGGQQ"
     gene            complement(22250..22590)
                     /gene="trbJ"
                     /locus_tag="J1Z28_002307"
                     /pseudo
     CDS             complement(22250..22590)
                     /gene="trbJ"
                     /locus_tag="J1Z28_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
     gene            complement(22520..23065)
                     /gene="trbB"
                     /locus_tag="J1Z28_002308"
     CDS             complement(22520..23065)
                     /gene="trbB"
                     /locus_tag="J1Z28_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBA7717136.1"
                     /translation="MPLTKSLLFTLLLTAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     GTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNINTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(23052..23333)
                     /gene="traQ"
                     /locus_tag="J1Z28_002309"
     CDS             complement(23052..23333)
                     /gene="traQ"
                     /locus_tag="J1Z28_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBA7717137.1"
                     /translation="MRKYKFSLPRLDITGMWVFSLGVWFHIVAHLVHSKPGMAFFLAE
                     LIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMAKQQEGGHASH"
BASE COUNT         5325 a         6651 c         5621 g         5948 t
ORIGIN      
        1 cagtgatgat aaccaattga tgcttaagtc cgccgtcggt ccttatatta acgtcccgca
       61 ttaatgccag aacgttgtca ttaaccagga gaatatcacc gatgtctcat gaacatgccg
      121 tttcattccg gcacaacaac tggcaggttg ctgccacgct ccgtttacct gaaggatttg
      181 atgaccggca taaatacccg gcaattgtct gtgctcatcc catcagcagt tgtaaagaac
      241 agacatccgg tgctgtctac ggtcggaaac tgactgaagc gggttttatc acactggctt
      301 tcgatgcttc cactcaggga gaaagcagtg gagaaccgcg cttcagcgaa aatccctcca
      361 cgcgtgtgga agatttcagt tgcgcggcag attatctgat gacgttaccc tttgtggatg
      421 aagagcgtat cggggtttta ggggtttgtg gtggtggtgg gtatgccatc agtgctgcca
      481 ccatagacca tcgtttcaaa gccatagcca ctgtggttgc cgctaattat ggtcgtatta
      541 tgcgcgaagg cgacggcagt ccggatgcgg cgctcagaac gattgaggct ctggggaaac
      601 agagaacggc tgaagctcgt ggggctgagc ctcttatcgt cggttatatt ccggccagtg
      661 cagaagagct ggcgcataca ggcattgacg atattgatat gactgaggct gttgattact
      721 acagaacccc acgtggacag caacccggtt caccgaataa actgcggttt accagtgttg
      781 cacaaagcct gagctgggat gcgtttgcct ttacagaaaa actgctggta cagccattac
      841 atattgttat tggcgatgtt cctggaggct ttggctctta ccgtgacggc tatgaacttt
      901 atcagcgtgc ccgctctgca gaaaaaacac tgcagattgt gaaaggggta tcccactatg
      961 atctgtatga tcgccctgaa gcgacatcgc aggcactggc aggaataata ccattcttta
     1021 ataaacattt aagcggataa gagacattat ctcctgttta tccggcatga actttatgga
     1081 cgagtaatgc cggacatttt ctataatgac agagttacat ttatacaatg tccggcaggg
     1141 cattgaaggg gcgaaagaaa atacctgggc gaagccgctg aacatcattc tggcgatttc
     1201 aataaaggta cttgctcgcg ctctgtcatt ttctgaaact cttcatgttg catttcgatt
     1261 ttccttctaa gatcataact cgcatacctg ttctttttat gtatatctat atctattttt
     1321 gtgacgagtt caaaccacaa tgttcgcact ccccatgcag cgttatgaaa catttccgca
     1381 acggctatag aatctgtatt aactgcttca gccatgtagt cgctaatacg ttcagcggta
     1441 attttcgtta tttcatcgta attcatttta tctcctacaa gattatttaa tggaatgcaa
     1501 aatatctggc ctgataataa tgttccagaa ttaatatcag caggctatgt taagttgtcc
     1561 ccacacacaa tctacctgtt ttctcgtgtg tgccacaggc gcaatacata aatagtctgg
     1621 ccattaagtt cgtaacggat ttcgtactcg ccagcaaaaa tccgtctgac ctccctgggt
     1681 tcgaactgaa acaactgttc tcccatacgt ggatgagtta acagaatgac cggtgcctgt
     1741 gtcagggact gtaccgttct ggcggcggca ggtttactgg ccagcaccag aaaatcatat
     1801 aaccgcgcca aatcagaaag cgccttactg gtccacttca gttccatcag cgtggcaccg
     1861 gtaacggatt gtcagtactg aggctgtccg cccaggcctg tacagcctga tggtcgataa
     1921 catgtctgga tgtcacatcg tccagggctt ccagcgtcag gcggttacgc tcctcctcct
     1981 gggcaagcca tgcagaaagc gcctgtttga taatccagcc ccgggagcgt tccagtctgg
     2041 cggccatctg gtcgacttta tcagccatag gtaatggcac atgcgcggta attacttttg
     2101 ttgcttgtgc ggtattgttt ttcatttgca tatccgatta gttgttaatc atatttaatc
     2161 atagtgatta ctttcagacg gaacaaggca aataatgcgc gtcagcgcat gtaaggcggc
     2221 atacggtagt tacatgtagc acaggtgtga aaccgccgaa gcccggcgta agccggtact
     2281 gattgataga tttcacctta cccatcccca gccctgctaa gccatacccg ctttcagcta
     2341 tgatagagct tctgtgcgcg gtcggagtgg ccccgacgag ggtttacccg aagtcggggc
     2401 gtatctccgc gttagcggac cgtcagggcc gcttacgagc gtgtatttca aatcttccag
     2461 ccagaagact gacagcagcg aagatttcta gttacaacat tcataattaa aagcgactct
     2521 gttccggccc tttgggccgg ggcggggccg cttttcagtt atgagggagg ggcgttgtgg
     2581 tttcagtttt gcgcggaacc ggggtttttc cggaggttgt ctttgtgtgt tgtaactaaa
     2641 gtggctccgg tcggggcccg ccgcttgcgg tgggaggtgc ttttaaattc tgagcggcgc
     2701 ggctttagcc gctgcaagcg aaggcctctt tgtatttgtt cacagaagtt tcagagataa
     2761 agactttccc tgcgggaagc aacgcagggg attaagtaac tggggtaaga ttatattgca
     2821 ggtttgctgt ttctggtttg cagcttctta aagttctttg tttgcggatt tatgtgggta
     2881 actgaaacaa cgtggacaag ttcagatgac tgatgcgtgg ttttatgggc agatgtgccc
     2941 ctctgagaga gatttaaggt gattgtggcg gattttgggc tgaacggaac cagcgccgga
     3001 agcccctgag gggctggcga tgattcagtg ctgaagggat ggtgctgttt agtgcggagg
     3061 cggggcagcc agcgtcagtt caagctgata cagctccctg atggccagct gctgtaagcg
     3121 ttccggcgtg caccagtagg cttcatccgg cggcatacgc ttcaggatgt gctgtgacat
     3181 ctcgtaaatc tgctggtcgg caggtaattt cttcagacct ctggcgcgct tactggctgc
     3241 cgcttttgca cgacggtgtt tcagcgcatc caggctgcgc tgcagatacc agcgttttct
     3301 ggccgcatgt acggagatat cttcatcctc acgcaacacg ccttcccgga tgagttgctg
     3361 gcggatttca ctttcacaca gccgcttctg ctgctgttcg tgaagtttaa ccatgtcgac
     3421 gcccagcatc tgccagccag ccggtgttat ccagacatag cgtggcagac gctggcggtt
     3481 ttcacgatcc cacatcgttt cctctgaaac acccagcaca ccaaaacgca cctgttcggc
     3541 cagcagacgg gaaaggcggg ggaccgtcac ttccagttcc ggaataacct ttcctttgct
     3601 gtctttcggg ctgatttctc tggccagacg ggaaacactc atccccacgg tgtgtgtgcc
     3661 cgcatcactg aagctgatca gaaccggcca gatggcatcg aggaggtgct gtttttccgg
     3721 acggaaagca tatttgcgac cagcgaacgt ccgtttctgc acgtaaaaag gatgtcgtga
     3781 catgcgcata cgcaggctct gttcgccagt caccgggtcc tttttcacca gacgattata
     3841 ggcatacccg agctgcccgc tcagcgcctt atagcgcgca ggacgatacc aggtcggatt
     3901 ttcgcatttg ggtttggtgg agtgctcacc acgacggcgt ttcgtgcgct tcctttccgg
     3961 ttcaaccagg aaagaggtcg cccttcccgc ttcatagtct tcccagaagc ggatttgttc
     4021 ttcgggaggc aactgagccc agtggcctgg acgggtattt ttaatcacca tacccactcc
     4081 gtaagacagc cagacgtgct tcagtcagcg ccaacggaaa tatccctaaa aactcaaaaa
     4141 aacgaggcgt caacgttatt gtgcagcgct tattgtaaaa atctgatgac ggggaacaga
     4201 ctataaaccc aaaatcttct tccagtgatt taattgctct gacagctcgc tcaatggcta
     4261 aatgaccatg agcatttttt ctggctaatc cgcaatcaag tgcaagttcg gttatagaac
     4321 gatgcacttg atttgaaaca ggatcgtaat agaagcacat accttgcaac agtgcatcaa
     4381 tagctctgcg acgaagaacg gaaggcttgc gtttacgttt accattagcg cgcgcgcacg
     4441 cagaagctat atcgaattca aatgtttcgg taaaccccac cgctttgccc attagcttct
     4501 cacagaacgg cagcgtcctg gcaccttcac gtggtgtgaa taccggattc gggttcttta
     4561 cctggcggta ataagtttgc tgaagatcag tcacaccata ctgcacttac aatgcgcaga
     4621 aggagtgagc acagaaagaa gtcttgaact tttccaggca tataactata ctccccgcat
     4681 agctgaattg ttggctatac ggttttatga ggccccggta atctttctag tcgccaaact
     4741 tgaagaagat tatcggggtt ttcacttttc tggctcctgt aaatccacat cagaaccagt
     4801 tccctgccac cttacggcgt ggccagccac aaaattcctt aaacgatcag taatctagca
     4861 tccgcaattc aaatgttcaa gaaagtaact tagttacctt ctattcacat catccatcgc
     4921 agctttttct ttcaaaatcc agtgctctat catttctttt tgagttaagc ccgaatcttt
     4981 acaaagctgt atcaaatcat ctttgagttc actctggatg aaaacattaa tcgctttatg
     5041 tgtccccttt ttccgggaaa cagatatgcg ttgtttctcg gctccggtta acggattacc
     5101 ttttctgtaa atgcgtttcg gtgaggaagt tactgcattt tcgatctgcg acatcccgcc
     5161 tcctcaatac ttaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg
     5221 acttgagaat tgtgacgaag atctgtgttg gcgcacaaat caacgggaat tactgtcgtt
     5281 taatgtgatt taaactatga aatagtatgg ttttcagttg tggaaacgcc ggggacgggg
     5341 aaaacttgct ttttcccgtc cccggggttg gacaactgag caacgcgaag gcgtcagcta
     5401 cgatgttccg gtgactgctg atcagcccct gttgtattca gccattctcc ggtcatctgc
     5461 agcagttttc tggcatcgtg gcttctgagc aacggaatac cagcctgcct cagaacctct
     5521 tctagaagaa tatcccggcg cctgcgttcc ggtcgtaaat ggcctgcatc atccagctct
     5581 actgctgcaa caatactgaa cgaacgccgc tcaacgataa ccacatcaac atgccactga
     5641 gacaccatcc tgaataaccg ccaccactgg cgtgatcgtg gccggatatt cccgttcagc
     5701 tggacgatat ccgcgacccg tacctgcgga cacagatacc accgcttcat atccacaatt
     5761 ctgaacagtc ctttcataaa actgacttcc ctttctgtca gcagcgtctt ctgacgttgc
     5821 cagtattcac cgctggacag tattcgctct gcattaccgg tccgtatacc tgccgcatgc
     5881 aattgcatca gcaaaggaga atactgattt acacttctga ggataatccg gataagtaac
     5941 gctgcaataa tcagaacaat gcgaataaaa ataatcaact tcattattta tctgctcttg
     6001 gcaattaatt atgctgaatt attaccttac atatttccat atctcttttg gtatcctgtc
     6061 atataacttt ccggtgatta actccgctaa ccggtggtct gctgctgaat taaatgattc
     6121 acgttcactg gtcgataaac tgtctctgtt tttatgaata attttttcca gcgtattgac
     6181 tgatgaacat cgccgtaact gatacagcca ttcctgtttt gtttttgcca tttttattcc
     6241 cgctgagaac agatgaatga taagattgcc atcgttttat ttacggtatc tccatatcta
     6301 gggtggaacc ggatcagcat ctgaccagag cccacgtttt tgttgtctgg cttcgttctg
     6361 aaggacaggc agcacgggat ctgtgtttta ctgctcatat acccaggcag ccccggcccg
     6421 gaccataaac tgattaatat tcattccgtc tggtgcataa acctgcccga gaatacgacc
     6481 ataacgatct cgctgaaaat atgtgacggt aaccgttttt cctgcgacca gtgatttcat
     6541 catgtctgtt gaccaccggc cataatcctg tttcttttcc ggtgcatcaa tatttaccag
     6601 cctgatccgc accgccttcc gtgagtccat tacctcgatg gtatcaccgt ccagtacacg
     6661 aacaacccgt ccatgaatat ctgcagacaa taccggccat gaaaagataa ataataccag
     6721 tggaatatat ttcctcatgt tattctccat atcaaataaa tggcacctgt atttcagccc
     6781 ccctgaatat cgcagcacac attgaataaa accttatttt gtgaaattaa tcacaaaata
     6841 taaaatcgcg acttaatacg gacaaaactc tgtgctactc agaactgtcc ggtcatactg
     6901 cccgaccgga tataaaaaaa agcaaatctc ataaactcct ttcagccttc tgactcctgg
     6961 agtaatgaag gctgcttttt tcgttacacc cttccccggc agcgccggtg gcagtgttca
     7021 gcccttttcc gggcaaactg ttgccagtct gtccggggag ctatgcaggc agcccgtcgc
     7081 catgcttctg ccgcttccat ccagcgtcca ctgacctcca gaataacggc ccgcccgccg
     7141 gcacgctggt aacgccagac ctgaccgttc cgtgaactgt ttctgaatcc attcattttt
     7201 ctgctcctcc cttttagtcg tcagtattat tgtggtaaat aattcagtgg actggcttcc
     7261 tttcgggtac ggaagttatt tccttttaag ccttattgtt tcagcctcct cacctttcgg
     7321 ggaggccttt ttatttctca tcaagcacgg cctgaagttc tgcctttatc cggttctggc
     7381 ggcagatttt atccagacgc tctgccgcat acgattcctc ctcctgagaa atatgctccg
     7441 tcacataccc ttccgtctca taccggcagg caccggcttt catggcacac agatagcttt
     7501 ctgaacgggt gatggccttc agcgccctgc gcagtttttt atgcgagagc gggatattcc
     7561 gctgcgccac gtcttccagt aacacgtccc ggataccgca ggccagaagc cggggggtgt
     7621 ctccgtcaaa taatcccggc caccagggct tcagggtgtt cacggcctca tccagtgagg
     7681 gcagagtcag ataaatggac agcgcctgtc tggcctgcgc tttttttgcc gccagctctg
     7741 cttcccgggc cgctttctcg gccagtttct gctttttcac cttccatttt ggtggcgtgg
     7801 tgacattaat gatggttttc cggctgcgga caggcgtctc tccttctgtt ttccgcttca
     7861 gtgtcagtac cggtcgcttc tgctctgtca tgctgtgttc tacctctgac gaatcatcct
     7921 cttgcggcca aagataccac acccgttaca gcgccagaac gcccagcaca gccagatgac
     7981 aggcatagaa cgtcgggaaa aaatctccgg gccagaaacg gggtaatgat ttccctgcac
     8041 acgacaccag accaacggtc agcaccgtca tcacaagacc agctacagcc gccacatcac
     8101 tggaggcaag gttaagcgcc ggaatcacgg ccagcaggca tgcaaccagc gccagacgtt
     8161 ccgttctgtc ttccgcccgg tacagccggt ggctgactgc cagcatcagc agcccggcaa
     8221 tgccgtaact ggtgccggac aaaggccccc acagtgccat catgagaatg gccgctgcag
     8281 tacgccacag gctgcgcgtt tcacaccatg tcagcacctg tgccgccact gcaaaggcaa
     8341 acaggatatt cccctcatac cagggaaagc cggccagata atatgcgaac tgggcaatca
     8401 ccgcccagcc ccacagccgg ttaatggccg gttgccggat atgcgcatgg cgggacagat
     8461 tcagccccca taccagggca aaaagcggga aggctccacg cccggccaga aacatccacg
     8521 tctggtccag atgcagaatg cggtttgcat ggtccagtac catcagcacc agtgccaccg
     8581 ttttgatgat atcccgctgt ccgggggacc agaccagcaa cgactgcaac caggtatcag
     8641 tccgggcagc cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc atcagcgttt
     8701 atcttcctgt atcagtctcc gctcagggtt ttctctttct gcaggtcacg aaccatatcc
     8761 cgctccatct gctgcagacg ctcctgcaac aggttttccc gggcaacctc gcggaccgct
     8821 tcccgttccc gttgtggttc acgcagtaca ctctccggca gcgcggcttc ccgttcagtt
     8881 atggcagccc ggtcacgctc ctgcccggta atctccctga cagcctgctc tgttttgccg
     8941 tccggcaggt caggtttgtt ctctgccatt ttacggacaa tttcatctgc gcggcgttcc
     9001 gtttcacggc ggatggcctc ttcagcctgc cgctgtgcca gtacttcagt cgtgaccggt
     9061 tcaccgtttc cggctcccgg ctggacactg ttgtccggga tatcccccca cacgcgacca
     9121 ccggtgatgg caccgggatt ccacggacgg ccttcaccgg cgattcttac caccacacca
     9181 ctgtcaggat tatcacgggc aatccggaca ccatcctgca tattatcagc cagcaggctc
     9241 tctccgttac ggctgccctg cagggccacg aactgcgcat ccccgctgcc tttcacccgt
     9301 ccttcaccac tgaatccccg cagcccgttt ccgtcatccg tggtcagtgg gttcagccag
     9361 atacctgctg acctgccgtt acggtcaaac gccggcagtg ccacatacgg ttgtggatat
     9421 ttgcgccccg gtgcaataaa ccgtgcagga ctgtctcccc cggccagccc cgcctgacga
     9481 agaacggcac ggcctgccgc cacgtcccgc agctcccgcg ccgtactgaa cagccgctct
     9541 gcattcatga cctcccggtc cggtttcggc tccagcacat cgtgggcagt tcctttctgt
     9601 acggcattgt taatggcatc cgtccagccc tgacggttat cggtgtacac ctgcacatgc
     9661 tgcttcatac gcgacagggc cacgtaggct gactcaaagc ctgccatcag tttccggtta
     9721 ccttccgtgc cttcaagcgc gatggcaaag gtttcactcg ccccctgcgc accgtgagcg
     9781 gtgatggcat aggccaggtc aatatgttgc tctgcctgct cctggccggg gcgaatcacc
     9841 cgggtctgct gtccgtccga cagcgtgaca ctgtcaccgg aaactgctgt caccgtccag
     9901 acgctgttgg ccacataacc gcgctcccgg tcactcttcg tgaagcgcat ccggtcaccg
     9961 gtccccaccc ggatggtgtc cggggtgtac agtgtgacgc cttcagccac cgcctcccgg
    10021 ggcgaaatca gccgcgtgtt accttccgca tcctgcaggg ttatcagccc gtcatccttg
    10081 ctgataccga caatccggtg atacacatta tccaccaggg caagtgcatc ctggttattc
    10141 tcccaggtgg agagacgacg cagctccccg tcgcgtatat tcgctgtgtt caggacaggc
    10201 accatgacct gctcttttcc cagctcaccg gccttttccc gtgcatcatg aatcaagctg
    10261 ttcagtacgc gtctgtcttc attcaggtgc gtaacaatca gcgtctgctc ccgtgcttcc
    10321 ggtgtcctgc aggtataatc gcggacaatg gcttcataca gtgtcatggg gacatccgga
    10381 aaagcctcac ctttctgcat cgccttctgc tgcgcttctg ccagtttcgc ttcctggctg
    10441 tgactgaact ccgtcacgga gtgctccggt gcccatgcgc cctcctgacg tggcacctga
    10501 gacggtttca cactctcaag cccggacagt gccctttcca catcccggtt aatcaggctg
    10561 tataccgcct cccgcagttc cggcgtctga cgcacaatct ccttcatgat ggccacatcg
    10621 gcagcactgc gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc ctgcagctgg
    10681 tccgtgtcac cgctggctac agctcgaccg ccaccggccg caatcagggc gtatgcccgt
    10741 gccatgtcgg tattgcccac cattgagctc tcatcgagca ggaacagcgt gttgctgaaa
    10801 tccggtgttt ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga cgccagtgtc
    10861 tgtgcatcca cgccggcgct gcgcatctcc ccgaccgcac ggtgcgtggg gcccagccca
    10921 acgacacggg gacgctcact ctccggcagc atgttcacag ctgacatcac cgcccggaac
    10981 tgtgtggtct tacccacgcc ggcatagccc tgcaccaccg tgaaacggtc ggacgtttcc
    11041 agtatcatac gggtggcggc acgctgtccc gacgttaaca tctccatgag ttcgccgggt
    11101 accctctcca tcagcggcgt gaccgcctcc ttaccttcga gaatatggcg aagaatgctc
    11161 ttttctgcct catacgacgc acgggaaacc agcaggcctg tgccatagcc ttttgccaca
    11221 tccacataca gtaaatcacc acgttttatc tgcgcattga tttcccctcc caggtcaata
    11281 aaactggttc cttctgcagc aaacgacttc gcctctgtca gcaggtccac catgctgaag
    11341 gccaggtttt tactttccag cacaggaagg gccagatgaa tggcctgctg tgccggcgtg
    11401 tgaagcccgg ctttctgcag actgatagca gtttccagcg gtgtctcacc ggcccgcgtc
    11461 tttatctgct cagaaaccac cgtaaaggag ggatggcggg caagtttttc cgcagtacgg
    11521 gtttcatcca gagaggaata cagccggaca tcacgaccgc tgcgggccag accgttcagg
    11581 gtggcgttgt ccattgccat ctgtgtgacg gaggcaaaaa ccgtcgcact gtcgctgacg
    11641 gaatacccgg gcgtttccac ccagccattc tccagcttca gtgccgtgaa cggtgaatca
    11701 gccacaggca gggtggccgg ttcagcccgc cccggcacaa caaccgtcat cgcatcttca
    11761 ctgacggatg ccacctgcag gcggtcaccg ccggagacgc ggagtccggg aattttccct
    11821 gtcaccctca gacgctcgcc gtctgccacc ggcatttttt ccggccggaa cagcgaccag
    11881 ctgctgtcca gggaggaaat acgcaccacc tgcgtttcac cctgcgcatc ccgcagggtc
    11941 aggctgtgac tctgcgccgt cacccggtcg ataacatagc ggtcatgact gcgtgtctcc
    12001 gggttccact gatccatcac catccccgga cggtacatat cccgcagata acggctccgg
    12061 ctgtccagcc agaccggtga gagggcggtc atggttacct ccgggtgtcc gagcacgccc
    12121 tgtgttttca gctcactgcg aatggcctgt gtcagtatgg cctgttcccg taccccgctg
    12181 acctgtgcca cgctctcttc tccggctttc acgctggccg caaaatctcc tgccagccgg
    12241 gcatagcgga cattacggtc cggttcactg atgatggtgg ccggtcgctg ttctcccccc
    12301 tgccagcgat atgtgttcac cccggcatcc ttcatggcca tcagcgcact gcctgtaccg
    12361 gttcgctgcc cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc accgtccagc
    12421 agggttaacg tctctttcag ggagagtttt tcgccctggt caacgataac ggtactgccc
    12481 ggtgtgaagg tcatgccctc ctgcagctga cgacgtccgg ttatcagctc accggacagc
    12541 cgttcatcct gcttcaggtt catctgcgaa cgacggtcag cagcgataat ctgcacctcc
    12601 cgcccctgct cccgggccat catgaccagt tcagccaccc gctcacgctg cccggcagca
    12661 ccgccctgcc cggacacaat ggccagcgac gggcgatcct gtgccagaac actgacggca
    12721 tcgctgtaac cggccgtccg ggggacactt ttctccggat gtacggtcac ccggttctgt
    12781 ttcatgatgt cacgactgag cgcccggact gacagctcat cgagcacatg aatcccggac
    12841 gtgaacagcc ccttctcacg atcgaggggg ataagctgct cacggctgat ggcctcatcg
    12901 ataccggcgc gtgcccgttc aatcacacca ttttccggcg gcagtatgcc gactgtcctg
    12961 gccagcacgt ccgtatacgt gaactgcact tttcgttcac ttaatccggc aatcgcctgt
    13021 gtcaccgcct gctgcacatc cggcccgtcc tgtgaagctg gcccgggcgt cagcgtgcgg
    13081 agatccgcac gctgatccgc cgcgtcacga tatgcccgga tgtctaaccc ggtttccttc
    13141 agcgtctgca tccactcggc cattttgatc tccggatcga catgctgttt ggatttacgc
    13201 gtatccagcg ccgccacatc ccgggatttc agcgaggcgt cttccccgac ggcctcccgg
    13261 atagtctgtg agcgtccgga aaaggcctcc accggtacac ccggcatttc ccacataccg
    13321 tgcttaccaa ccacttcagt ttcatagccc agcgcctcaa cctgctcttt cagtttttcc
    13381 cggtagagcc tgccaaaggc aatctgatta gcgtacacat tctcaatgaa ccccgttttc
    13441 cccactttgt cactgctcag tgtcttccat tcgccgttat gctgcgtgac attagccacc
    13501 accgcatgcg tgtgtaactg tggttcctga tcgcgactgg tgtcgtggtt aaacagtgcc
    13561 atcaccagat taccggtcag caccgtttct gactgtccgt ccgtcatcac ccgtgtggag
    13621 gccagcgcct ccacctgacg gacagcaaaa tccacggcct gattatgtgc atcaatcagg
    13681 cgcttatcgc cacctaacat ggccatcatg gagacacttt tgggggcgga gaaggtcaga
    13741 tcgtagccgg gacgatgcct gttactgcca tcctgcatgc ggcttagatc cgctccgtcc
    13801 ggcagcctgc cctccagaag acgggtaaaa acatccttat cgacactgcc ctgcagcccc
    13861 agctgttcag cccccctgcc ggcccagcgt tctcccatgc tgcccagcac atagtaatta
    13921 tccttgtcgg tataatagtt accggcactt ccggccgatc tgacctgcgc gatactcatc
    13981 atcagaaatc atctcccggc tcaacatcct ccccgcgctc ccggtgcaca ttaatgttca
    14041 cctcttcacg acgctgcatc tgctgctgga tgtccggatg attttcctgt tgccatgcct
    14101 cataagcggc catatccacc acttcaccgg attcactgat cccgggtggc agttgctgtt
    14161 caatctcttc ttccggtttc attttcagct cctgctcgat cccccctgcc ggaacattca
    14221 cacctgaatc tgacttctta tcgttgatgg caggagacac cggctgttgc ggctgttgcg
    14281 gctgttgcgg ctgttgcggc tgttcagcct gagtcacgtc ttctccggaa acaacctccg
    14341 ggacatccgg ttcgaagagg ctgaccatct gacgaccttc tgcttccctt gcggcaagta
    14401 cggcactcag acggttctcc atttccgggt tgatgtcacg cggaataaac tccggagcga
    14461 ccttcggtcg tgtctgatat ttcagggaga gttttactgc cggatacggt ccgggcaggg
    14521 tgacataaca ggtcagatcc ggcagagact gaatgtcgga ataactgacc agcgtctggc
    14581 gctccatatc cttaccggtc gataccccgt cacgtaccgg atcagcaccg taggaatact
    14641 gctcgctggc tttcaggtgc tctttctcac caatctcacc ggcagcgaac tctgcaatct
    14701 tatggctggg agaacggaaa aaggcacggg tgttcatgac gtcaaacagc gtggctgccg
    14761 ctttctcacc gtagatatct tccagctggg cataggactg gataccaaac acatagcagc
    14821 ctccgaactt acgggcttcc ggcaggatct ccaccaggtc cggcagtttg tgtaacgtgg
    14881 gtaactcgtc acagaagaac cacacgcgac ggttacggtt ttctcccatt gccagcagac
    14941 cacgaatggc aatggacagc cacatggaga tcaccggttt cagggaggca tgggtgtcgg
    15001 cgttcgacga aataaatagc cagccgtttt tctgatcttc ccggacaccc cgcatccagt
    15061 cacggatggt gaagggttca ccgttatgct caatcccctg caggtaacgg atggctttca
    15121 cgtagttggt cagcacagca cggatggaaa tcgccgtttt ctcaattttc tcttccacta
    15181 ggttggccgc cggtgaatta cgcaggtagg tacgcagttt ttcaattttg atggaaagca
    15241 gtgtgtccac cagtttgctg tagctgcggt tagggtcatt acgcatcaga tacgccgctt
    15301 ccgcaaaaat ggtacgtcct gaaccctgcc agaacgggtc ctcttttgta cccatcggga
    15361 tcagagtatt tgcggtatta tcaaaatccg gctgtgtcag gcactccttc cacaaatccc
    15421 atgcggcaca gcgggcatcc agcggattca ggattttatc gatggaggga tcatagtaac
    15481 ttttaacaaa ttcccccgaa cggtcataaa tcaccaccat atcaccacgc tgacgggcgt
    15541 agttggccag acgacggata acctccgact taccggcccc gaccgtgccg tgcagacaga
    15601 agttctggat ttcggaatcc cggataatcg gcaggtcgcc aatccgtata tcggagtcct
    15661 tgccgtcttt tttcagcatc cgggcaacgt ctttcggatt gtctgtcagc tgacgaccac
    15721 ctgtgacttc attttcgctc tgttgtttac cctgacaccc cagaatccag gagacaacaa
    15781 agaaggtaat caggcaaata accagtgcca caactgtggc cagaacgaat gcggaccata
    15841 gctgctctcc gcaccagatc atatattttt catgcagtac ctgagccgcg ttcatccgga
    15901 aggttttgcc gtaatactgg atctcatata ccggctggga cttgattaaa tcccgcatgc
    15961 cttccagcgt ggtacaccac cagtaaatac agccgttcac aaacgtctgc cagcttattt
    16021 ttacccataa aaccagacca acgagtatcc agaaaaaaat aaacaggcag taaagcatga
    16081 tattggcgat ctggctgaac atgcggatac gcatagacgc aatctgaccg ccctgggtca
    16141 tatcctttgc gttaaaactc atgatgatat tccgaagaaa gaaagtcagt cggactgatt
    16201 attaatgatg aaaagattaa taaccgttaa attccggctt cgccggcaac acttcacggt
    16261 cgcacaaaga atttgcacaa ccgctcagtg ttttcatatt tatttcggaa acgccgggga
    16321 gaaataaaat ttattttatt tctttccggg gttggacaac gagcaaagcg aggcgtcagt
    16381 caggaggccg gtcagaccag cctccggaag ataatcagag aatatttgcg attgatttgg
    16441 ccagctggtc ttcgagaaca ggcttcgcct cttcaaattt caggttaacc ttgttcgcat
    16501 ttgagaccac acgggtctgg tatttatgct ggttacctgt ttcagtactg gtctgaattt
    16561 tcgcacctga tgtgccctga cgcagggcgg caacattatc cgttgtcacc gttgccttag
    16621 tacgctctgc aatctgcaca tccgtgatca tggtatagtt cacatcttcc accatcgcat
    16681 ctgcagccat acccaccaga ccggcagcaa ggcccacacc gagtgtggca ccggcagaac
    16741 tggaattata tccggtgata ccggcaccta acgctgcacc aactgctgcg ccttcataac
    16801 cacggttcag ccatccctga gactcccgca gatccatctt atcggccttc agcacattcg
    16861 cctgaatcca gtagtaggct ttatccggag aagtcaccac ctgataccct tttgctttca
    16921 cagcatcagc aattttgccc tgcagcccac tcatgtcttt atcagacgtg tttttgatct
    16981 gcagaaatac cgtgcgttcg ctggcgggtt caagccagat ggtctcactc atctgagtct
    17041 tcacctcaag gttacgcttc ttgattgctg tgctcatcgc accacaccct gaaagggcca
    17101 gagtagaact gaccagtgca accatcatca atttttttgt tttcatcaaa ttaccttttt
    17161 tacaaactac agttttctta ttgcctgtca taatagtcct gccaacgagt cccaaaagta
    17221 tttcccgctg aatcaaagcc tccatgcata ggatatccgg ttgaagggtt atagtagatc
    17281 gttgggttct gttcaatgcc cccgaagtct aacttttcac tcccatcata ttggtctttg
    17341 agttgatttt gcccccattt ttttatgctt atgcaaccac taactcgcct taattcgtat
    17401 agtgtactaa tgataaaaac catcaggctt aataacggag agagtaagga agatactggc
    17461 atcgttatat accaaacgcc tttactaaac ttaaactcat ccgacacatt ccgttttttt
    17521 cgtatttgag agaacaacca gataaacagt ccctgccata gcactatgaa gaacagcata
    17581 aaaactgttt cggcattccc ccaatcagcc agataacagg cgataaactg tatcaccgtt
    17641 accagagcaa tatgggctaa acatctcatt atcatctccc aaacacagca ttattgctta
    17701 tacttatcct gatactcctt tgcccttttc atcagttcct gtggactgtc agccccgggc
    17761 attctttcct gagccgactt ttcttcatta tatttattat tctgagataa tgcctctgtt
    17821 ttatggtgat tctgcagttc agaatactgt cctcttacaa tattttcctc gccacgtatg
    17881 ctgttctgag tatcacctat attcccttta tattctgtga ccatattatc aacctgatgt
    17941 tttacatcat tacggatatt actgtcctgc gttctttgct caataatggc ctgatgtccc
    18001 tgatggtcag caataatatc ctgactgcct ccacccgaag gtacgctctc cattccttta
    18061 ccaatatcgc cacgggattc ccgccaggca ttatcaacac taggctgtat ctgttcctgc
    18121 acaaaagacc aggccattgc ccgacggcgt tctgcaatct ccggcgaact ggtatttgtc
    18181 agaatagctt cggcatcctg cggtgcgtgt ttcatcacat actgtgcaaa ctgttgcgac
    18241 aaatcttcgc tcatctgccc actcatgctt tcagtacggg atgccatttc agcatattca
    18301 tggctgcggg tcatattcgt cgtgtactgg tcgtaactct gtttcgctga attcagggct
    18361 gcagacagtt gatccacccg ggaatcagca ttattgtcag tatggctgcc ggattcactg
    18421 actttgcgac tggtaaagta atcgctggcc tctttaaagt ccttactggc tttagcgtca
    18481 atatcatgac gagcatcata gctggcccgt gaaccactgc tggcctgatg cgcatcatca
    18541 tcactccagt caatgccagc ccgaccaccg cctctaacgc ctaatcctcc accaacacca
    18601 agaattttag gtttaacccc ccattcagca tgagcatctc catacatccc ggcagaagct
    18661 ctcgtacttc tggatgccag ttcctgagtc gcctgctcat tactgatatt atgcgccttc
    18721 gcatagcttt ccactgcact gcgcatacgg ctggccatca tggagtcctg agcactcatc
    18781 gtgctgtcag caccaccggt gacagaatca ctgctccccc ggttagaacc aaactgactg
    18841 agcgtgttcc aggcactggc aatactgctg ctgaacccat gcagggcact ttctgctctg
    18901 ttcgatgcct cccgggccat ttcctgttgt gccgccgcaa tctgacgcgt tgcattgata
    18961 ccgaccggta aacgggacat cgctccgcta gcatccatca ccatattacc gtcacgggtc
    19021 tgtgtggcag ttgcgccact gccggtctgg tacatcatct gaccaaacga cgtggtgctg
    19081 ttggtgctcc agctgaagcc gttcacgttc tccgtctgca tgttgccgta ggagtagtta
    19141 ccgtcaacca cactgcctgc tgcactggcg gtcgggctga tagcagagga agcgaaatgg
    19201 ctgtacacac tggaaaatcc tgccccgagc cccctgacca tcatccagga taatggcggg
    19261 atcatcatgg aaagataccc ggcagtggaa gccagatcgg agtatttcag ttgtatctgt
    19321 gaaatttccg agagcaccac cggtgcacca ttctgcttcg cataaaatgt catggcgctg
    19381 ttcagaatgg cgtacaacat tggccatgac tgcaaccaca tcagggcaaa cacatagcct
    19441 ttcaggacgg acagcgtcag tttgttgaag acagccgcca gtaccagaag cgggaaaata
    19501 ccaatggcga tccccgtcag gatggtctgc gtcatgggca atgtccgcat cgccacatgg
    19561 ccgatggaga catgtgccag acgttgcttc tccatcgatg atgtggtggc cagattcacc
    19621 aggctggccg aatcaccatt acgtgcggca taactggtga tcccctcctt cagcgcattg
    19681 atggtgacat tctggcggat gatctggctg gctgatttac ttgagccata gaagtaactg
    19741 taactgtcgc caatcagcgt actgaacaac aggttcggat cagggcgtcc accaaatagc
    19801 tgctgggcat aataatgcca ggtttttccg ccactctgtg tgtccagatt cagcttgtct
    19861 ttcagcgaaa ccgacgcgtc cttacaggtc acgaaattat tattgctgtc ataaacgccc
    19921 cgcagcggac tggggcggaa aaaaatcagc gtgtagggat cagcggagac catcagttcc
    19981 tccagcgtgt atttgtggtt caggtaaata tcccccatca cgcagtgctg cacataatcc
    20041 tggaaaagat tggcgatttc cggattacgg gacaaaaagt cggtgctttt tgataccagt
    20101 tttgcgccaa acagcatccc cgttttgctg taggtgacac tgtccggttg cgtgaagatc
    20161 atctcgtaac tggccaccat tgcatgcccg atacgcgtcg tcagtgaaag tggcatcgcc
    20221 agtccgaccg gcacattatc cacccggtga actttgacca ggtcactgtt atcaataatc
    20281 tgcaccgatg tgcggacatt aaccagcagg ctgataagca caaacacggc cacccagccc
    20341 agcaaatcca tcacgttgtg ccgctgtacc cacatcacgg ccaccgcgag aacagacaat
    20401 gtgagcgcaa ttttttcaat ggaatcccac gtcctggttc ccataaaggc ggcaatggcg
    20461 ttcaggttat tccgcaacca ctcaccaccg gcaatcacat aaacttcatt cacagcgtgc
    20521 tccctccgaa gtgatagttg ttctggtaac gactgagcat gcgggcggaa agctgctgac
    20581 gcatgtagct catctgacga tcgacaacca gcagcgcatc ctgctgtacc tgcacctgtg
    20641 actgaaacgc cgcaatctgc cgggtggcat cattcatgtt gtcgttaata tgctcgataa
    20701 ccgcttcgtc ataatttccc gtggccacca tcgcccgggc ctgctgtatc agctcctgaa
    20761 tgtactgcag caggatgtcg taaccgatat agtccgtcag ctggtaaatc atgctgttgg
    20821 aaacaccgag catctgcggg tcaaccaggt atttgaagac ggggatggtg gtactggaaa
    20881 taaagccttt ttccttgtca tccagaggcg tgtcactgac cgccttgttc tgaatgctgg
    20941 ccagaagttt agtaatctga gacttcagtg cattatcccg gctgatggtc accggtgtgt
    21001 ctgccaccac tttcaggcat ttatcagaat cgttgcagtg ataaattttt gctgtgccgc
    21061 cttccatcat ggcccggata attgagcggt cagtggttct ggctgacagc ggtatgattt
    21121 cgccgttggg accaaacacc agtgagccgg tcagcgtcat cacaaattct ttcagctctt
    21181 tgttgccgtc aaacattctg tttttggaaa gcgcattcca catgatgttg atgtttttgg
    21241 tcacccgctc cttatccttg tcgctggctt tatccctgac gctgtcagat ttcccgccaa
    21301 cggtacatcc ctgccgggaa gccgcccagt cagcaaaaat attgctctca ccggcaatgt
    21361 cctgacagac tttctgctgg gacacctgcg tccgggggaa aagcccgccg ataatcccct
    21421 gtgcagcctg gcaggaactg aggttcatac tgttaatgtc gcttgccatt ttctgcacga
    21481 agtcttttgc ggtttttatt tccggcaccg ttgtctgcag ggcaaggtca aaaaagtaac
    21541 cggcagcatt gctcataatc tgcttaacaa aacgctgcaa ctgctcacca ttaataaaac
    21601 tgaacgagcc gagataggcg tcgatgccgc cacatccggc attgatatcc ggcagcgtca
    21661 tggaaatcag ctgaacattt ttgacctgag tacgggcata cagggagcca ccgtaagcat
    21721 agccggcggc ctgcccctgc caaacgcccg gctgtgtggt attggaggcg aagcccagct
    21781 tgttaaagaa ctgattcatg tcgctgttca catccgctga cgctgcaggc gtcaccgtca
    21841 gtaaggcggc acaaagaata aaaagaggct taatgcgaag cattattgct gccctccctt
    21901 aatttgttct cccggcgcat acggcgtttc aacaggaata tcccgatatt tccgtatatt
    21961 aattcgtaca ttgggaacag aaaaaaatgt aacaagaaac ccgtctgcaa gcgcatataa
    22021 cgtatacggg atggcataca tcaggatgtt ccacatataa gtcgaattat ttaattccgg
    22081 gtccgctttc cacgaatcaa tacaggcatc aaaaataatg ttcataactg tccataaaac
    22141 aattccggca gtcaggaata ccagagacaa tccagcatac ctgtcagtaa ttctggaaat
    22201 gtatttataa tctcgttctg tacttctgat tttgagttca ggattatttt tattttctct
    22261 cattataagt tccctctgat atttccgatg attcattcag accggtcatg cggctgtaat
    22321 atatttcaga caccgaaatc catcccacaa ttgccccaca aaaaacacaa cagaaacaga
    22381 aaaggtaaga cacagcgaaa cgacaatttc tacagggctg gcgggataaa aaagccccag
    22441 acggataaag gggatattca ggctgacgat tattcccgtc gcgatgccgg atattccggc
    22501 aatgagtaac accacctgct tatttcgcac cttttttccc tccgtacatc tgcagaacgg
    22561 tatccatccg cgccataaaa ccggcggcat ccgttgcccc ctgtaaaagc ggtaatgcct
    22621 caagcgtgtt gatgttgacc aggaaggtgg ttggtgtggc caccgggata ttcgggaaga
    22681 aggtctgcat cacgtccggt ggcaccggca atgcctccgg aaaggcggta tcaccctgac
    22741 catccagggt gtagggaaaa acggaaaagc catactgctg tgccagctgt ttcagtaccg
    22801 ggtcaaactg gtgacagtaa gggcaatgcc cctgcataaa cagcaccacc ttccagtcgg
    22861 ccaggttaac ctgcctgccg ttgctgaggc ggaaccagcg gggggccggc ttttctgtcc
    22921 tggctgacgt gcctgctgcc ggttgtgctg gctgcgcggc cataccctgt ggattccaga
    22981 gtcgctctat ttcatcccgg gtggaggcct gcacggcagc ggctgtcagt aacagggtga
    23041 acaacagtga tttagtgaga ggcatgtccg ccctcctgct gctttgccat catggcctgc
    23101 tgcctggctt ccagcgcctc acgctcttcc cggctgacgc gggcaatcca tgcatccagc
    23161 acctggtatg caccgaacag cacgagaata gccgcaataa gctccgccag aaaaaaagcc
    23221 atcccgggtt tgctgtgaac aagatgggcg acgatatgaa accagacacc cagggaaaat
    23281 acccacatac ccgtaatatc aagccggggt aaagagaatt tatattttct catatttaaa
    23341 acaccattat tattcagtat attgctgatt tcatcaatca gacacagtta tgttaattat
    23401 cacggattta atactatgag tttatttttt gacagaaaaa cactggaagc tatcagagac
    23461 attatccggg aaataatgtt aggcaatgtt gccattgtct tactgataaa catttcaccg
    23521 gactatatgg tacaaaagaa ctgtc
//
LOCUS       DADSZD010000067        22135 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.067, whole genome shotgun sequence.
ACCESSION   DADSZD010000067 DADSZD010000000
VERSION     DADSZD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.067"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            105..1577
                     /locus_tag="J1Z28_002310"
     CDS             105..1577
                     /locus_tag="J1Z28_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA7717138.1"
                     /translation="MSGNESIDAAHYDQVTTWPRDGLQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIH
                     TPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
                     AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     GAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDADFRSITRQLMINE
                     QARTLNPDLNIDAQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            1574..2590
                     /locus_tag="J1Z28_002311"
     CDS             1574..2590
                     /locus_tag="J1Z28_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA7717139.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVGICGTDIHAF
                     AGRQPFFSYPRVLGHEICAEAVSRGSQCQTAQSGQRYSVIPCIPCGECAACREEKTNC
                     CERVSLYGVHQDGGFSEYLAVREDNLVPLPDEVSDSAGALVECFAIGAHAVRRAEIKA
                     EQNVLVIGAGPIGLATAAIARAKGAHVVVADIDCQRRQHVVDHLAINVFDPTQEGFIA
                     ALSEVFGGELACVVLDATGNKASMSHDVNLIRHGGKIVFIGLYIGELVIDDPTFHKKE
                     TTLLSSRNATQEDFALVIELMRSNKIHENLMKNQAFDFFSVGEDYQRNVVENKNMVKG
                     VITF"
     gene            2601..3611
                     /locus_tag="J1Z28_002312"
     CDS             2601..3611
                     /locus_tag="J1Z28_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HBA7717140.1"
                     /translation="MTTVYVSEENLKSLVHHKLHTAGLDTDTTQQVTDVLVHADITGV
                     HSHGVMRVEHYCTRLAAGGLNKAPQFSIEQISPSVAILDSDDGMGHSALISATEHAIK
                     LAQQEGLGFVSVKNTSHCGALSYFAEMITNKGLVAIVMTQTDTCVAPHGGAERFLGTN
                     PIAFGFPVENSHPMIVDMATSATAFGKILHAKETGKHIGEGLAIDKNGYGTTDPYKIE
                     NLLPFGQHKGSGIALAIDALTGVLMNANFGNHIVRMYGDYDKMRKLASLVIAIDPKKL
                     GNPVFAKTMAKMVTELHAVKPAPGVEKVLAPNDPQMHYKEKCQQEGIPVPAGIFHYLA
                     EN"
     gene            3684..4667
                     /locus_tag="J1Z28_002313"
     CDS             3684..4667
                     /locus_tag="J1Z28_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBA7717141.1"
                     /translation="MKALRPLTASLMLLTSCLLISNTTLAKTTLKLSHNQDKSHAVHK
                     ALSYLADKTKEYSNGELVIRIYPNATLGNERESLELMNSGALQMVKVNAASLESFAPD
                     YSLFSLPFLFRDRDHYYRVLQSDLGKKILNSSESKGFVGITYYDGGARSFYSNKPITK
                     PEDLAGMKIRVQQSPSAIAMMKALGGVATPMAQGELYTALQQGVVDGGENNTVVYADM
                     RHAEVAKVYSRDEHTMVPDVLIISTDVLNKLGDKERTALLKAADESMMQMKDVIWPAA
                     EKEAYDKMKGMNATVVDVDKSALKERVKPLYDEFKAKDAQSAKNLELVESM"
     gene            4709..5191
                     /locus_tag="J1Z28_002314"
     CDS             4709..5191
                     /locus_tag="J1Z28_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA7717142.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNSH
                     "
     gene            5202..6506
                     /locus_tag="J1Z28_002315"
     CDS             5202..6506
                     /locus_tag="J1Z28_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBA7717143.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGVPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(6577..7989)
                     /gene="ftsP"
                     /locus_tag="J1Z28_002316"
     CDS             complement(6577..7989)
                     /gene="ftsP"
                     /locus_tag="J1Z28_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBA7717144.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(8064..8801)
                     /gene="plsC"
                     /locus_tag="J1Z28_002317"
                     /gene_synonym="parF"
     CDS             complement(8064..8801)
                     /gene="plsC"
                     /locus_tag="J1Z28_002317"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBA7717145.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(9036..11294)
                     /gene="parC"
                     /locus_tag="J1Z28_002318"
     CDS             complement(9036..11294)
                     /gene="parC"
                     /locus_tag="J1Z28_002318"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBA7717146.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLLEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHILMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11432..13039)
                     /locus_tag="J1Z28_002319"
     CDS             complement(11432..13039)
                     /locus_tag="J1Z28_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7717147.1"
                     /translation="MYTRNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDDGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSVTGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASTFLNTLKS
                     DSEENVGHWKNAQYDALLNQAAQTADATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKVH"
     gene            complement(13148..13630)
                     /locus_tag="J1Z28_002320"
     CDS             complement(13148..13630)
                     /locus_tag="J1Z28_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7717148.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPVPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(13683..14075)
                     /gene="ygiW"
                     /locus_tag="J1Z28_002321"
     CDS             complement(13683..14075)
                     /gene="ygiW"
                     /locus_tag="J1Z28_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBA7717149.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            14227..14886
                     /gene="qseB"
                     /locus_tag="J1Z28_002322"
     CDS             14227..14886
                     /gene="qseB"
                     /locus_tag="J1Z28_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBA7717150.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            14883..16232
                     /gene="qseC"
                     /locus_tag="J1Z28_002323"
     CDS             14883..16232
                     /gene="qseC"
                     /locus_tag="J1Z28_002323"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBA7717151.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLHDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            16342..16923
                     /gene="mdaB"
                     /locus_tag="J1Z28_002324"
     CDS             16342..16923
                     /gene="mdaB"
                     /locus_tag="J1Z28_002324"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBA7717152.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            16954..17268
                     /locus_tag="J1Z28_002325"
     CDS             16954..17268
                     /locus_tag="J1Z28_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBA7717153.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(17313..18200)
                     /locus_tag="J1Z28_002326"
     CDS             complement(17313..18200)
                     /locus_tag="J1Z28_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7717154.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRAVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(18197..19144)
                     /locus_tag="J1Z28_002327"
     CDS             complement(18197..19144)
                     /locus_tag="J1Z28_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA7717155.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLAACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(19155..20192)
                     /locus_tag="J1Z28_002328"
     CDS             complement(19155..20192)
                     /locus_tag="J1Z28_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7717156.1"
                     /translation="MNVGLRPLRVGKFSTLVCPKNLVLLGGLFLFAVGILIFGLMHGS
                     FSVPASEVGRALFSPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(20201..21184)
                     /locus_tag="J1Z28_002329"
     CDS             complement(20201..21184)
                     /locus_tag="J1Z28_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7717157.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(21181..21990)
                     /locus_tag="J1Z28_002330"
     CDS             complement(21181..21990)
                     /locus_tag="J1Z28_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7717158.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
BASE COUNT         5703 a         5667 c         5386 g         5379 t
ORIGIN      
        1 ttcgtacttt tacctttctc tgcaagcgtg atgcatatct ctgtttttgg tctagtgaat
       61 tatgtaaatt ggtatacaca caagccacca ggagtaagag cataatgtca gggaacgaaa
      121 gtatcgatgc cgcccactat gatcaggtaa ccacctggcc tcgggacggg ttacaggcag
      181 atattgtcca tattggtttt ggcgcttttc atcgcggaca ccaggctgtc tacacggatc
      241 tcactaatca gctttcggac acccactggg ggatctttga gatcaacctg tttggtgatg
      301 ctcaactgat cgaaaactta aatgcgcaaa atgggctgtt ttcggttgtg gaaacatctg
      361 catcgcaatc tacctcacgc cttgtgcgtt cggtaactgg cggtattcat acccccgtgg
      421 atggcattgc cgcagcaatc cagaaattga ctgaacctca ggtaaaaatc gtttcattaa
      481 ccatcaccga gaaaggctat tgtctcgatt cgcaaacgcg atcgcttgat ctcaccaatg
      541 gattaatcaa acacgatcta caaaacccgg atgcgcctca gtcggctatt ggcttgatcg
      601 tctgtgcttt gcaacaacgt aaagcggcag ggctcgccgc ttttagcgtg ctctcctgcg
      661 acaacttgcc agacaatggg catctgacgc gaaatgccgt tctcggtttt gcccgacaac
      721 tggaccagcc cctggctcag tggatcgaag aaaatgtctc atttccaggc acgatggtcg
      781 atcgcattgt tccggcaatg actgaatcgc aattcgcctt actggaaacg aaaaccggtt
      841 atgccgatcc ctgcgggatc gtctgcgaat catttcgtca gtgggtgatc gaagataatt
      901 ttgtgcgcgg acgaccggaa tgggataaag ccggcgcgat gtttgtcagc aatgttcagc
      961 cttatgaaga gatgaagtta cgcatgttaa atggtagcca ttcatttctg gcttataacg
     1021 gctcactggc tggctatgaa tttatctggc aatgcatgga agacgctgat tttcgttcca
     1081 ttacccgcca actgatgatt aatgaacaag cccgaacact taatccagac ttaaatatcg
     1141 acgcccagga atacgccgac ctgttaattg aacgctttag caaccgtaat gttgcacacc
     1201 gtaccgggca aatcgctatg gacggttcgc aaaagcttcc ccagcgagcg ctgacgccct
     1261 ggctgaaatt gcatcagcaa aaacaaaaca atgctgttct gtcactgctc gttgctggtt
     1321 ggttgcatta tgtcattgat gctgttgaga aaagccagtc tgtcgctgat ccaatgaatg
     1381 accaacttca ggcgctaata aaggaacaac aagacgcatg gcaacaggcg ctcgcattac
     1441 tgcaccttag cgccatattt ggtgatttaa gcaaccatca gccatttata aatgaaataa
     1501 aaatcgcctt tgcaaatata aaaaacaaag gcatcaaggc caccatcagc caattattat
     1561 cggatgagca gaaatgaaaa cattaatttg tcagcagcct ggcgttatgg aatatgtgga
     1621 aaaggatatt cccacaccag cagataatga agtgctgtta aaaatcaaag ctgtgggtat
     1681 ttgtggtact gatattcacg cttttgccgg cagacagcct ttttttagct acccacgtgt
     1741 attaggtcat gaaatatgcg ccgaagcggt ttcgcgaggc agccagtgcc aaacagcaca
     1801 atcaggccag cgctattccg tcatcccatg cattccgtgt ggcgagtgcg cagcctgtcg
     1861 ggaagagaaa acgaactgct gcgaacgtgt ttcgctgtat ggcgtgcatc aggatggggg
     1921 ttttagtgag taccttgcgg tacgtgaaga caaccttgtg cctctccctg acgaggtcag
     1981 cgacagtgcc ggagcattgg ttgaatgttt cgccattggt gcacatgccg ttcgtcgggc
     2041 agagatcaag gctgaacaaa acgtactggt gattggcgct gggccaatcg gtctggctac
     2101 cgcggccatc gccagggcta aaggggcgca tgttgttgtt gctgatattg attgtcaacg
     2161 tcgccagcac gttgtggatc atctggcgat taatgtcttc gacccaacac aggaaggttt
     2221 tattgccgcg cttagtgaag tgtttggcgg cgaactggct tgcgtagtcc tggatgcgac
     2281 gggaaataaa gcttcaatga gtcatgatgt caatcttatt cgtcatggcg gcaaaattgt
     2341 tttcatcggt ttgtatattg gtgaacttgt tattgacgat ccgaccttcc ataaaaaaga
     2401 gacaacgtta ctcagcagcc gcaatgccac acaggaagat tttgcgttgg tgattgaact
     2461 gatgcgcagc aataaaattc acgaaaattt aatgaaaaac caggcgttcg atttctttag
     2521 tgttggcgaa gattaccagc gtaacgttgt agaaaataaa aatatggtca agggtgtgat
     2581 cactttttaa gggaacaaat atgacaactg tttatgtcag tgaagaaaat ttaaaatcac
     2641 tggtgcacca caaattacat accgccgggc tggataccga caccacacaa caggtgacag
     2701 atgttttagt acatgccgat attaccggtg tgcattcaca tggtgttatg cgtgtcgaac
     2761 attattgcac ccgccttgct gccggagggt taaataaagc cccgcagttt agcattgagc
     2821 aaatttcacc gtcagtggcc attctcgact ctgacgacgg aatggggcat tccgcattaa
     2881 ttagcgccac tgagcacgct attaaactgg cccagcaaga gggtctcggt tttgttagcg
     2941 ttaaaaacac gtctcactgt ggtgcgttat cttattttgc agagatgatc accaacaaag
     3001 ggttggttgc tatcgtaatg acgcaaaccg atacctgtgt ggctccccat ggcggcgccg
     3061 agcgcttttt gggaactaac cccatcgcct ttggtttccc ggtggaaaac agccatccga
     3121 tgattgttga tatggcgacc agtgccacgg ctttcggcaa aatacttcat gcaaaggaaa
     3181 ccggaaaaca tattggtgaa gggctggcga tagataaaaa cggttatggc accactgatc
     3241 cgtataagat tgaaaacctg ctacctttcg gccaacacaa aggttcaggc attgcactgg
     3301 ctattgatgc actgactggc gtgctgatga atgcgaattt tggcaaccat attgttcgca
     3361 tgtatggtga ttatgacaag atgcgtaagc tggcaagttt ggttattgcc atcgatccga
     3421 aaaagctcgg caatcctgtt tttgcaaaaa ctatggcgaa aatggtcacg gagctgcatg
     3481 ccgttaaacc ggctcctggt gtcgaaaaag tgttagcgcc gaacgatccg caaatgcact
     3541 acaaagaaaa atgccaacag gaaggtattc cggttccggc aggaatattc cattatctgg
     3601 cagagaatta atcactattt tttattataa cgatagaaaa gcgctgtatt tatcgtttct
     3661 gttatattaa ccaggagcag gatatgaaag cacttcgtcc tctgacggca tcgcttatgc
     3721 tgttaaccag ttgtttatta atatctaata ccacactggc aaaaacaaca ttaaaactga
     3781 gtcataatca ggataaaagc catgctgtcc ataaagcatt aagctattta gcggataaaa
     3841 ccaaagagta ttctaatggt gaattagtta ttcgcattta cccaaatgcg acattaggta
     3901 atgagcgtga atcactggaa ttaatgaact ccggtgcatt acagatggtt aaggttaatg
     3961 ccgcatcact agaatcattt gctccggatt acagcctttt cagtctgccg ttcttattcc
     4021 gcgaccgtga tcactattat cgtgttctgc aaagtgattt aggtaaaaaa atacttaatt
     4081 catcagaaag caaaggtttt gttggtatta cgtactatga cggtggtgcg agaagttttt
     4141 attccaataa accgattaca aaacctgaag atttagcggg aatgaaaatc agggttcaac
     4201 aaagccccag cgccattgca atgatgaaag cactcggtgg tgtcgctacc ccgatggcgc
     4261 aaggcgaact ctacaccgca cttcagcaag gagtggttga tggcggagag aacaataccg
     4321 ttgtctatgc cgatatgcgt cacgccgaag tcgcaaaagt ctattcacgt gatgaacaca
     4381 ccatggtacc tgatgtgtta attatcagca ccgacgtgtt gaataaactt ggtgataaag
     4441 agcgcacggc attattaaaa gcagccgatg agtcaatgat gcagatgaag gacgtcattt
     4501 ggcctgcggc tgaaaaagaa gcctacgaca aaatgaaagg gatgaacgca acagttgttg
     4561 atgttgataa atccgcttta aaagaacgcg tcaaaccgtt atacgatgaa ttcaaggcga
     4621 aagatgcaca atcagccaaa aaccttgagc tagtagaaag tatgtaaccc taatgggcag
     4681 gtaattcctg ccttttcatg gaagacttat gcgaacactc actcatattc ttaataagat
     4741 ccttgccgga tgttgctgca ttattctcgc cattatggtg ttctgcgtaa cctggcaagt
     4801 tattgcacgt tttattttta acgctcccag cactgtgctt gatgagttca cccagatttt
     4861 atttatgtgg atgattttgc ttggcggtgt atatactgcg ggtttaaaaa aacatctggc
     4921 cattgatttg ttggctcaaa aacttccggc tgcatctgtt ctgacgcttg acagttttat
     4981 ccaaattatt attacggtct ttgcggttat tttcatgatc tatggcggga atattgttgt
     5041 tgaaaaagca caacatgttg gacaaatctc tccagtatta aaatggccaa tggataaagt
     5101 atattgggtt atgcctgtca gtgggcttat tctggtctgg tattcagtaa tgaatattat
     5161 tgacaactat cgtaaacgga attctcatta acgagggaaa tatggaattt gaatatatct
     5221 accccgtctt aattttattt ggcagttttg ccgtcatgct ggcaataggt gtgccaatta
     5281 cttttgcgat tggtctttct tcgctgttat ctattattac tgccttgcca cccgatgccg
     5341 ccatttctgt tatttcacaa aaaatgactg tagggctgga tggctttacg ctattagcca
     5401 ttcccttctt cgtgttagcc ggaaacatta tgaataccgg tggtatagcc agacgactgg
     5461 ttaacctggc gcaagcatta gttgggcgtc ttcctggctc actggctcat tgtaatatcc
     5521 tcgcgaatac actgtttggt gcgatttcag gttcagccgt tgcgtcagcc gcagcggtag
     5581 gtgggattat gtcaccactg caagaaaaag agggatatga tccggcgttt tcagcagcgg
     5641 ttaatattgc ctctgccccc attggcctga tgatcccacc gagcaatgtg ttgattgttt
     5701 attccctcgc cagtggcggg acttctgtcg ctgcgttgtt cttagctgga tatctgccgg
     5761 gcatcctcac cgctgttgcc ttaatgtttg tagctgcact ttatgcacga cgtcaccatt
     5821 acccagtggc cgaacgcatc aattaccgtc agtttttaca ggtattccgc gaatcaattc
     5881 ctagcctgat gcttattttt atcattattg gcggtattat cgcaggggta tttacgccca
     5941 cagaagcatc agcaatagcg gtaatttata gtttagccct ggcgatgatt taccgggaaa
     6001 tcactcttaa gaagctcaat gatattctgt tagattcggt agtaaccagt tcaattgttc
     6061 tgttactggt aggctgctcg atggggatgt catgggccat gacgaatgct gatgttcctg
     6121 agttgatcaa cgaactgatt acccgcgttt cggataacaa atgggtcatt ctttttatta
     6181 tcaatatcat tctgttgatc gtcggtacct ttatggatat cacgccggcg atcctgatat
     6241 ttacacctat tttcctgccg atcgcccagc atctggggat tgatccgatt cacttcggca
     6301 tcattatggt tttcaactta accattggcc tttgcacacc acctgtcggc accattctgt
     6361 ttgtggggtg cagcattggt aaggtcagta tcgacagggc aataaaacca ttactgccga
     6421 tgtttctggc attgtttgta gtaatggcaa ttatttgtta tttcccgcag cttagtctga
     6481 tgttgccagg attattttcg acctgataca caatgaagac tgcatactga gtaattttca
     6541 atgccccacc actctgtggg gcattgaatt ggtccattac ggtaccggat tgaccaacag
     6601 ttgcccaatc gagccacggt ccgccatttc cagcgtctga ctgttgaagt agaacgggaa
     6661 gtgcgcccag gaaggctgac cgaaataaac aagcagctcc acttgtccgt caacccaaac
     6721 ggtatctttc cagcctctgt cttccgggaa cggcatcgcg ccattcacgt tacggatctg
     6781 gaacatcacg ccttcaatat ggaacgcttg cggctcgtcc gcgcgcaccg tccagcgttc
     6841 ccacgttcct tgctgcgcag taacatcaat acggttgacg tcccacaact gcccattaat
     6901 ccccgggtca tcacccagac tgatatcacg acttcgaatc ggcgaaccgg ccatgatttc
     6961 agttggcagc aagcgcatcg gaagactgtc tgtgaccagc ggcagaaggc cggttgggcg
     7021 cagcgttagc accagcgtag aaaccagaat gctggatggc tcaaagaagc cacgaatacg
     7081 atcaacaatg ctcgccgctt cgccacaggt aatcgacact tcatcaccgt tgctcatatc
     7141 caccagaatc tcacgacgct cgcccggtgc cagcgaaagt tgcttcaccg atacaggagc
     7201 gggcaggaat ccctgatcgc cagaaatcac atgtaacggg cgaccatcgc tcatctgtag
     7261 ttgatagcga cgagagttcg acgcgttcaa cagacgcaag cgcacccagc cacgcgagac
     7321 ttcaacgtac gggctttgta caccgttaac cagtagcgta tcgccaacaa agccgccgct
     7381 tcccggttcg ttatattctg gcgtaccaaa gttatccagc cgtttatcct gaataatgac
     7441 aggaaaatca tccacaccat aatggttggg gataggcagc gatttactga cttcatcttc
     7501 caccagccac attccggcaa ggccgttata aacctgctga gccgtgcggt tgggagtatt
     7561 ggcgtgatac cacagagtag ctgcgttctg gcgaatgggc agtacgggtg cccagtcagc
     7621 atttggcgac atcatccgtg ccggaccgcc catcagcggg ccaggtacct gtagcccggc
     7681 caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa ataagcttaa catcgtcgcc
     7741 cttccagacg cggatagtcg gccccaggta acgaccattg attccccaaa ccgacgcgcg
     7801 tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc ataaacagcg gttgcccacg
     7861 gcgagattca agcagcggcg gaacgggtag cggttgctgt tgcccggctg cgctggcctt
     7921 caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc tgaatgaact gacgccgact
     7981 gagtgacata tttgctccat gtaaaactgg ctaaagtaat accgtgcagg aaagtgttcc
     8041 ccgcaaaaat acagattgcc gacttaaact tttccggcgg cttcgcgttc tgcgacttct
     8101 ttatcgagct cggcgatttt ttgttccatt atcgaacgac aatgggcagc caactcgcga
     8161 acctgatctt tgccatactg actgacgtca attggcggca gcatttcgac aatcaccaga
     8221 ccgttgtgta gccgattaag attaatctta ttcgaagttg tagagacgca cacgggtata
     8281 atcgggacgc ccgccgcaat tgccgcgtga aatgccccgg tcttgaacgg cagcaggccg
     8341 cgaccacggc tgcgggttcc ttccgggaac atccagatgg aaatacggcg ttttttgaag
     8401 tgattcacta cttccgcaat agtgccgtga gctttagtgc gattgtttct gtcgatcaat
     8461 aagttgccgg ttaaccagta caactgcccg aagaagggga tccacagcaa gctcttttta
     8521 cctaccgtca ctgtcggcgg ctgcacaatg ttcgatgccg tcaccatgtc ataattgttc
     8581 tggtggttag caatatagat agcattgccg tagctttcag cgtctgcagg tttgcggcac
     8641 tccactttca ggccgaaaag cggcgcaaga cggccaaaca tatgcccaaa ggtggccaca
     8701 tgtttcgggt tacgcgggct gaaaaggcag taaatggagc cgaatacaca gactaagatg
     8761 ctgtaaatca cggtaataat aagacgaaag atatatagca taacaccctc tgaaggtctg
     8821 agagactggc attgtacgtc acctgaaaat caggttaggg cattgttaaa agccgctctc
     8881 tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta
     8941 tcgtttccgg ggattgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct
     9001 tgcaagcggg aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg
     9061 gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta
     9121 ccgcggcgtc cacgttcgcc agcgactttc tgtagctctt ccggacgcag tttaattttg
     9181 cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccagg
     9241 ccatcctcgc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc
     9301 ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatc
     9361 gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc
     9421 ggtaaggtga ttaaagcttt acctgcacgg ttacgcgcca ccagatcgtt aaaggtgcag
     9481 acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcaga
     9541 atatggtcaa cggtcgctcc aggcggcaac gttaatttgc cggtgagcgg ctcgccctga
     9601 ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca
     9661 aacactaccg gttggttgct cttacctttc accgccgctt tgaagctatc gcccgcttta
     9721 taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc
     9781 gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct
     9841 tcttcgcgtt ccagcaacgg cgaacgacgt tcgtcaccgt aggcttgcgc gtctgcctgc
     9901 agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg
     9961 tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca
    10021 agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc
    10081 gacatcagcg ccggtttcgg ttcatcttca ttacgaatga tctcaatcac ttcgtcgata
    10141 ttgagaaacg ccaccagcaa accttcgagg atatgcaggc gcttgaggac tttctccaga
    10201 cgatagttca gtcggcggcg cacggtatcg cggcggaaca ccagccattc ggagaggatt
    10261 tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga
    10321 tagctctttt ccagatcggt ggtagcaaag aggtggttca tcacctgatc catatccacg
    10381 cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc
    10441 agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg
    10501 cgcgcacctg aaacctgatg cggcaatgcg ctgataacca ccgcgccatc ttctttcttc
    10561 cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg
    10621 cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc
    10681 tgatcgagcg tggttttcgg ttggtcgatt aatgcgattg ccgcctgagc cacttcacgc
    10741 aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc
    10801 aaaatgtttg gcagacgggc aggcagcatt ttcggctcct gcaaagtgcc gtcgaagttt
    10861 ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac
    10921 aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag
    10981 ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc
    11041 gcttcataac aggcactatc gccgtgcgga tggtatttac ccagtacgtc accgacggta
    11101 cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca
    11161 atgcgccgct gaacgggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc
    11221 acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct
    11281 gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata
    11341 ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc
    11401 cgccggacgt gctccgacgg cgataaaggc atcaatgtac cttgatatac aactctttgc
    11461 tgtagacata atcctgggga ttatgcagcg gaaaaccgcc aacgtagggt ttaagcagtt
    11521 tgattaacgg ctgatagtag atgggaatca gcggtgcctg ctggttgatg atcacttctg
    11581 cctgctgata cagcgcatta cgctttgttg catcagccgt ttgtgccgcc tggtttagta
    11641 aggcgtcata ctgcgcgttt ttccagtggc cgacattttc ctcgctatcg ctcttaagcg
    11701 tgttcagaaa ggtggaggca tcattgtacg tcgcatccca cgactgccga gacaacatga
    11761 aatcaccggc tcgtcgggca tcaagataag ttttccactc cattgtgcgc agcgtcacct
    11821 gtgcacccag ccattttttc cattcggaag acaacgctat cgcggtcttt tcatgcagat
    11881 cgtacttgtt gtagaacagc tcaaagcgta gcggatgaga ggcgtcgtat cccgcctgtt
    11941 tcagtaagac ttttgccatc gcgacgcgct cactcattgg cttttgcagt tcatcgaacg
    12001 tcgtcgcgct aaagcctttt acctctggcg gcgtcagcgt ggttgcgggc gttctcaacc
    12061 ccagtacctt ttgcgcaata agctgtcgat caaccgtaag atatagcgcc cgacgcaccc
    12121 gcacatcgtt aaatggcggt ttctcaaggt taaagttgta atattcgctg ttcagacgcg
    12181 ggataattcg tagctcgcca ggcagtgatt tttcaatggc gggaatttgc tgcgccggaa
    12241 cccaggtgag atcgacctct cccgcgcgat agcggttata gccggtgacc gaattatcca
    12301 gcgccagata ctcaacctgt tgcaatactg tatgttgcgc atcgcggtac tttggatttt
    12361 tgcgtgcagt aatcttttcg ttaactaccc atttatcaag cacaaaggca ccgttgtaaa
    12421 ccatgttctc tggcttactc cagctatcgc catgtttagc gatgacatga tgaggaaccg
    12481 ggaacagcgt cggccaggcg agcatcgtcg tgaaccaagg aactggctgc tcaagcgtaa
    12541 cttcaagagt acgatcatcc gtcgctttga cacccagcga tgtaacatct gctttacccg
    12601 caacaatagc cgcggcattg ttaatgtgcg cctgtgccag atagccagca aaagggcttg
    12661 ccgttttcgg gtcaaccgcg cgctgccagc cgaggacaaa atcctctgcc gtcagaggct
    12721 gaccgtctga ccactgcaaa ccgctacgca gatggaaaat atagcgcttg ccgtcgtcaa
    12781 gtatttccca gcgttcagcc tgagcgggct gcacctggcc ttcaccgtcc atccatacca
    12841 gaccttcaaa cagatccagc acaatctgcg cggcagtatt ctcctctacc ttttgcgggt
    12901 cgagcgtgcc tgggtcgcta tgattgttgt aacgaaagac ttgttgcggg gcgagcggtg
    12961 tgttggcggg aacgtcagca gcgtagagag gagccgcact taccaggctg accagccata
    13021 ataaatttcg cgtatacatg gtattcctta ccggagaaat tataagcccg acaacaatac
    13081 gcctgtggaa ttgtttgctc aaacttatcg cgagtgattt ggctcacact ccggtaaaga
    13141 ttccccatca cgccaacggc acataaatat ccgtttccag tcgccgctct gtcatccctt
    13201 cggcaagatt ggtgtagtga aacaggatcg gggctttgcg cattttttcg ccgcttgcag
    13261 gtagccagtg gcgaatgatg ccccatatcg tgtgtgaaat atcgtccagc tcgccaacat
    13321 ggcgggccac agcataacgc ccaccggtaa gttcgccatt actcacgcca taacgattat
    13381 cgggaacagg ttcgctaacg ctgccgcaga tatcaaagcg aaacgcttcc ggtgcggtgg
    13441 tggcagggtc gtcccaggcg acgccaaaag tctggctttg gttaacagga gaaagccccg
    13501 tctctttacg ccacatgata aatttcgcca cgctgtagtt gagcaactca gggctacagc
    13561 ggtgcggcaa catcgccaca gggattgatg ggaaatcgat aatatttaca tccagtgtca
    13621 ggtttgtcat tttgcctccc tgcgaaatta ttcccgggaa cgcgtcccgg gagcggtaac
    13681 aattacggat ttactttgcg gatctgtttg acgtcaattt caacagaatt ccagtcttta
    13741 tcgacttccc cctgaatctc aaccgtatct ttcggcgtca ccgtcacgcc gttccagcgt
    13801 ttgtgatcga tatccacatt gatagtaccg ctggcatctt tgaacacgta gagatcgtca
    13861 gagatgcgtt caacgatatt gccgcgcagg gttacccagg tgtcgtcacg cagggatttt
    13921 gcgctttcta cggtcgttac gctgccgttc ggcccctgga atcctccggc ctgactttgc
    13981 gttgccgatg ggccagaaaa acctccctgc tctgctgcca taaccggtgc actgcacagg
    14041 gccattactg cgattactgc tgcgaatttt ttcatgttta ttactccctt taatgtctgt
    14101 ttccgagcat ttaacaagat agtccttaac aacttcttaa gggaaaaaaa taaaatttaa
    14161 tgctgtacag agcgcgttac aacacggttt actggcagca aatacggtta tcgcagggat
    14221 gaaaaaatgc gaattttact gatagaagat gacatgctga ttggcgacgg catcaaaacg
    14281 ggccttagta aaatgggttt tagcgtcgac tggtttacac aaggtcgtca gggaaaagag
    14341 gcgctatata gcgcacctta tgatgcggtg atcctggatt taaccttacc gggcatggat
    14401 ggtcgcgata ttttgcgcga atggcgagaa aaaggtcagc gtgagccggt actgatcctg
    14461 accgcgcgcg atgcgctggc ggaacgtgta gaagggctgc gtctgggagc tgacgattat
    14521 ctgtgtaaac cttttgcgtt gatagaagtc gccgccaggc tggaagctct gatgcgccga
    14581 accaacggcc aggccagcaa cgagctgcgc cacggcaacg tcatgctcga ccccggcaaa
    14641 cgtatcgcca cgctggctgg cgaaccctta acgctgaaac caaaagaatt tgccctgctg
    14701 gaattactga tgcgtaacgc tggtcgggta ctgccgcgca aactgattga agagaaactg
    14761 tatacctggg acgaagaggt caccagtaat gccgttgaag tgcatgtgca tcatctgcga
    14821 cgcaaactcg gtagtgattt tattcgtacc gtgcatggta ttggctacac attaggtgag
    14881 aaatgaaatt tacccaacgt cttagtctgc gagtcaggct gacgctaatc tttttaattc
    14941 tggcctcggt gacctggctg ctttccagct ttgtcgcctg gaaacaaaca acggataacg
    15001 tcgatgagtt gttcgacacc caactgatgc tgtttgccaa acggttaagt acgctcgatc
    15061 tcaacgaaat caacgcggcg gatcgcatgg cacagacgcc aaataaatta aaacacggtc
    15121 atgttgatga cgatgcgctg acttttgcca tctttaccca cgacggcaga atggtcctta
    15181 acgatggcga taacggagaa gatattccct atagctatca gcgggaaggt tttgctgacg
    15241 ggcaactggt cggtgaagac gatccctggc gttttgtctg gatgacctca cctgatggca
    15301 aatatcgcat cgttgtgggt caggaatggg aataccgtga agacatggcg ctggcgattg
    15361 ttgccggaca attgatcccg tggctggtcg cactgccgat tatgttaatc atcatgatgg
    15421 tactactggg tcgtgaactc gcgccgctga acaaactggc gctggcacta cgtatgcgtg
    15481 acccagactc ggaaaaacca ctaaacgcga cgggcgtacc cagcgaagtg cgcccactgg
    15541 ttgagtcgct aaatcaactg ttcgcccgca cacatgcgat gatggttcgt gaacgacgct
    15601 ttacctccga cgcagctcac gaacttcgta gcccgttaac ggcgctgaaa gtgcaaaccg
    15661 aagttgctca gctctctgac gatgatccgc aggcacggaa aaaagcgctg ctccaattac
    15721 attccgggat cgatcgcgct actcgtctgg ttgatcaact gctcacgcta tcgcggctgg
    15781 actcactgga taaccttcat gacgtcgcgg agatcccgct tgaagatctc ctgcaatcgt
    15841 cggtgatgga tatttaccac acggcgcagc aggcgaaaat tgacgtgcga ctgacactca
    15901 aagccaacgg catcaaacgc accggacaac cgctattgct aagtttgttg gtgcgaaatt
    15961 tgctggataa cgccgtgcgt tacagtccac aaggtagcgt ggtagacgtc acgctgaatg
    16021 ccgataattt catcgtgagg gataacggcc ccggtgtgac accagaggca ttggcgcgaa
    16081 ttggcgaacg cttctatcgc ccacccggac aaaccgctac cggcagtgga cttgggctat
    16141 cgattgtcca gcgaatcgcc aaattgcatg acatgaatgt tgaatttggg aatgcggaac
    16201 aaggtggatt tgaggcgaag gtaagctggt aagggtaaga cttttgctaa attcgcaaaa
    16261 gactttgcac attttgctaa tttcaccgta ccgctctgtg acgtactata gtcggcaaac
    16321 gtctcacctt gaggttaaaa aatgagcaac atcctgatta tcaacggcgc gaaaaaattc
    16381 gcccactcca atggtcaact gaacgacacc ctgaccgaag tcgcggatgg cacactgcgc
    16441 gacctcgggc atgatgtccg cattgttcgc gccgacagcg actacgatgt caaagcggaa
    16501 gtacaaaact ttctctgggc tgatgtggtg atctggcaga tgcctggctg gtggatgggc
    16561 gcgccgtgga cagtgaaaaa atatattgat gatgtattca ccgaaggtca cgggactctg
    16621 tatgccagcg atggtcgtac ccgcaaagat ccatcaaaaa aatacggttc cggcggcctg
    16681 gtacagggca aaaaatatat gctttctctg acctggaacg caccaatgga agccttcacc
    16741 gaaaaagatc agttcttcca cggcgttggt gttgacggtg tgtacctgcc gttccataaa
    16801 gcaaaccagt tccttggcat ggaaccgctg ccgacattta tcgctaatga cgtgataaaa
    16861 atgcctgatg ttccccgcta tactgaagaa tatcgcaagc atcttgtgga aatttttggt
    16921 taactagaga tctggcttta gaaggagtta accatgatta cagtaatcgc agaaatccgt
    16981 actcgtcctg gtcaacatca ccgtcaggca gtgttggatc agtttgctaa aatcgttcca
    17041 accgtactga aagaagaagg ttgccacggc tatgcgccaa tggtggattg cgctgctggc
    17101 gtgagtttcc agtctatggc accggattct atcgtgatga ttgagcagtg ggaaagcatc
    17161 gcgcatcttg aagcgcatct gcaaaccccg cacatgaaag catatagcga agccgtaaaa
    17221 ggtgacgtgc tggagatgaa tattcgtatc ctgcagccgg ggctttgaca tttctaaaaa
    17281 gaggccggtg ctgctaccgg tctcagccca gattagccat tctttcttcc cgttttcagt
    17341 cctcgatgga aatcgttaat tcgcttaatg gttttaaccg cacgctgcgg cactgcggaa
    17401 gctaccgacc agacaatcat ttccagacac aacaataccg taccatgtag cggcgttttc
    17461 cctttttcat ccccgcgcgg aacatgaatc acgatactgg cgtctttgct aaaacgtgaa
    17521 tccagcgcgt ttgtcagcaa aataacaggg atccccagcc gcctcgcctc acgaagcgtt
    17581 gtcagccctt cccggtgcgc agatttttgc gccatcatga tcagaacatc gccacgttga
    17641 agcgcgatca gttgctcggc aaggccaata cccgtacggt ttagtgccgt ggcaggcaaa
    17701 cctatgcggt tgaatagtcg tgcggtatag tccgccagaa tacccgaagc gccaatacca
    17761 aaaatagcga cttgccgtgc ctgcaccaac aacgctacag cttgcgcaag cgcgtggcgg
    17821 ttttcaggct ctgacaggac attacaggta tacaaatgcc cttcaagaac aaagtcgatt
    17881 gcggtgttaa catcgctcgt caagttactc accgtcgtag acatcttttc gctggaacta
    17941 agggctggac caagccactg ttccagagtg cgtttcagat cacgcagccc ggcaaacccc
    18001 agggcctgta tagccctgat aactgtggca tcggaagtgt tcaacgtggc cgcaatttcc
    18061 atcgccgttt gctcgagtac cacttcacga tgctcattaa tgtagctggc gacagctctt
    18121 aacccaggcg ttaactgact cgcgcgagtg cgaaaacgct caccaaaaat atcaacccgc
    18181 actttatctt tctggatcat ttagcctccg gtagaggtcg ccctgcaatt tgtgattgta
    18241 tctgcgcagc catcagcccc agagatgcaa aggagttaga aattgcctct tcacgagaaa
    18301 tcagcacata acgcccggaa cgacaggcag ccagaaactg acaccatccc ggcatgactt
    18361 tttccattgc tgccagctca tcctgaggtt taccaccggt gtcaccgcgc caggttgcaa
    18421 aaacaaaatc ggcatccagt tcaggtaaac gctccgcgct cacatccatt cgcccacctt
    18481 caggaatgct ctcaatcagc ggcggaaaac gaaaacccgc atcgcgtaat acccgcccca
    18541 gcgaatgata gctatgcatc acattaattt ttccctggtt tgcctgaatc accgataccg
    18601 ttattttttg actgtccagc gtcgccttca gcgcattgat ttgtgcctga tagcggcgtt
    18661 caagtatcgc cagctgcgac tgcgttcctg taagttctgc cagcttgcga tagatttctg
    18721 gcgcgccccc tttgagatga tcaatgctca ccgtgggcgc aattttttca agttgttcaa
    18781 tgggcgtatt acgggtcggt tctgtgatga tcaggtcagg ctttgccgcc acaatcgctt
    18841 caatatcaat atcagcggta ccaataaagg cgatcgatga attatcaaag tcgacacctg
    18901 tcaataacgc gccagatcga atgaaatggc taccgtcagg ccgagttcgc ccgtggctgg
    18961 cgacaggagg tacaccgagc tcaatcagag gaatagtgat atcaagatcg tgtaaagaga
    19021 caattctctt tggatgagca ggaacagtga ctttgcgccc taaatcatca gtaaatacct
    19081 gaacaggttc tgttgcacgg gaaaaaaatg acaacagtat cagcagagaa aaaaataaac
    19141 gcatataact cccgttaaaa actgttcctg cgctgccaga gcagtagcaa aaaaaatggt
    19201 ccaccaatca gtgagataat gattccggca ggcagttgca aaggcaaaaa agccaaacga
    19261 ccaatgctat ccgccagaat aactaacaac gcccccgata ccgcgctccc cagcagtaat
    19321 gtcgtctgcc cgccacgtag aataaaacgt gatatatggg gtgctatcaa tccgacaaaa
    19381 ccgatgttac caacacacga aacgcagacg gctgtcagga ttatgggtgc ggcgacacgt
    19441 aacaacgcca gacgagaact attcacccct aacccggttg caacctgatg accaagcaga
    19501 gcgatatccg cagtcctggc ggtgaacagc aataaaagag cggctggcaa catccaacag
    19561 gctgaaatac cgactaacat ccagttagct gcatgcaggc ttcccgataa ccacatcaat
    19621 gcagtttgta catctcgaac atctgcagtg gtcataaata caccgatacc ggcggcgaaa
    19681 aaccaggaaa caccaatacc aatcagaaca aatcgcgggc gggaaatatc gcgcgcacaa
    19741 aaaatcacga ttaaagccac caacaggcca ccagcaagac cagccactgg acgccagaac
    19801 attcccagca ttggaaactg gaaaatcagc cacagcacag ccacgctaca tccttctttg
    19861 acgccgatta acccaggatc ggcaaggccg ttacgtgcaa tggattgcat tgcggcccct
    19921 gccataccta acatggcccc gcatagcaat gccatgatga ctctcggcaa gcgaatatcc
    19981 tgcacaatat aacgtgcgtc tgtgcttacg ttttctggag agaacaatgc tcgtcctact
    20041 tccgatgccg ggacggagaa agagccatgc atcagaccga atatcagtat tccaaccgca
    20101 aacagaaaca acccaccaag caacaccaga tttttagggc ataccagtgt cgaaaactta
    20161 ccaacacgta gtggtcgtag cccgacgttc attatccgac tcatttgaac atcctcgtcg
    20221 ccataatgac aaaaacgggc gcaccaacta acgccgtcat gacaccggtc gctaactcat
    20281 ggggcgtgaa aagcgtccgg gcaataatat ctgccagtaa taacaaaagt gcgccgacac
    20341 aagctgaaag agggagcaga acgcgcagat ccgctgatac cagacgccgg acaatctgcg
    20401 gtaccaacag gccgacaaaa ccaatggggc cagcaatcga tacggctgcg ccacagagca
    20461 acgcgatcgc aagcagggta aatgttcgcg tgcggagtac agagacgccc agcccctgcg
    20521 ccatgcgatc gcccagtgcc aacatgttaa gggagggtgc aaggtaaagg gctaacacaa
    20581 atccccccag agaaaaccag gcggacgtac ccagcgtcgc ccaatcctgc cccgctaaat
    20641 cacctgctag ccatgtccgc atcgccagca gtgtttgttc gtcaagaatc aggattgcag
    20701 ccgttattga ggacacgaac gccgatagcg ccacgccgca taaagtaact ttcattggcg
    20761 ttacccctga acgccctgcg gaggaaagca gcaatatcaa caggaacaat aacgcgcccc
    20821 ccgtagatgc cagcagtggg cgtccaaccg gaaaggccag tccaagcgca ctggcggcaa
    20881 cgacagcaag agcggctccg gcatttaagc ctaaaatatg tggctccccc aatgggttac
    20941 gaatgacagc ctgcaacaaa gcaccagcaa caccaagaga agcaccggta agcaatgctg
    21001 ccgctaaacg cggcaaacgg agtcttacaa ttacgttgtg atcgaagttt tgcggatcaa
    21061 aatgaagaaa tgcctgcaac accgtttgtg gtgcaataaa tcgggcacca atccctggat
    21121 ataccacgca tcccaccagc aataaggcga ggaaaattac agtaaccagc cctgtgcgca
    21181 tcatactttt tctacaccac gaaacggcat gaagaacggc tttcccgtca gaggatttat
    21241 tgatgcgtga acatcaacgt caaagacagc ttttaccagt tcaggcgtgc aatgctcccc
    21301 ctcattcaac acacgcacca ctttcccctg gcgtaaaaat atcaacgtat cgccataatt
    21361 gacggcaaaa ttcagatcat gcaacaccac gacgactgta cgcccgtgat gacgcgttaa
    21421 atcatgtaat aactcaagaa tttcaacctg ataacgtaaa tcaagccagg ttgtgggttc
    21481 atccagcagg atataaggcg tcttttgcgc caacaccatc gcaatccagc aacgctggcg
    21541 ctgcccacca gaaagttttt caacaggtaa atgtgcaaac tcctgcgtac cggttaattt
    21601 cagggcctct tctaccgcct gctcgtcggc gtcgctccat tgacgtatga aattttgcca
    21661 gggaaaacgc ccacgcgata ccagttcata aacggttaag ccctccggca ataatggcga
    21721 ctgcggcaag atcccaagtc tacgggataa cgctttcgtt ggttgctcgt gaatcgcttt
    21781 gccatcaagc aatatcgaac cgcccatggg ttgcaagata cgtgcaatgg tgctgagcaa
    21841 cgtcgacttt ccacagccgt ttgccccaac cagaacggtc attttttctg tgggaacgga
    21901 gaaagaaaca ccatcaacaa tgatcttctt gtgatacccg gcagaaacat tgtcgaggat
    21961 cagcccctgc ccttttgcgt tatgtgccac gtgtacgcca acttaatcaa agacaaacaa
    22021 aaataaaact cattctcctt tgagagtttg acgccacaaa atgtagtagt caagcagtta
    22081 aaagccacac aaaaacaagg taaataacag gtgaactatt tttatacata aaaat
//
LOCUS       DADSZD010000068        22116 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.068, whole genome shotgun sequence.
ACCESSION   DADSZD010000068 DADSZD010000000
VERSION     DADSZD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.068"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(353..1417)
                     /gene="phoH"
                     /locus_tag="J1Z28_002331"
     CDS             complement(353..1417)
                     /gene="phoH"
                     /locus_tag="J1Z28_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBA7717159.1"
                     /translation="MVTSCTGHVLDNQRATTRGDFSSGSHLVTLYFQPHPFFSCVTDA
                     VNGARSRFSAFYPKANYGLQGSQPSDVRAHNRAANGACDEYKQLKVLSMGRQKAVIKA
                     RREAKRVLRRDSRSHKQREEESVTSLVQMSGVEAIGMARDSRDTSPILARNEAQLHYL
                     KAIESKQLIFATGEAGCGKTWISAAKAAEALIHKDVDRIIVTRPVLQADEDLGFLPGD
                     IAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIGKVEIAPFAYMRGRTFENAVVILDE
                     AQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLPRGVRSGLSDALERFEEDEMVGIVR
                     FGKEDCVRSALCQRTLHAYS"
     gene            complement(1768..3039)
                     /gene="efeB"
                     /locus_tag="J1Z28_002332"
     CDS             complement(1768..3039)
                     /gene="efeB"
                     /locus_tag="J1Z28_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBA7717160.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNAKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEEGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(3045..4172)
                     /locus_tag="J1Z28_002333"
     CDS             complement(3045..4172)
                     /locus_tag="J1Z28_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBA7717161.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4230..5060)
                     /locus_tag="J1Z28_002334"
     CDS             complement(4230..5060)
                     /locus_tag="J1Z28_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBA7717162.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            complement(5280..6788)
                     /gene="putP"
                     /locus_tag="J1Z28_002335"
     CDS             complement(5280..6788)
                     /gene="putP"
                     /locus_tag="J1Z28_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBA7717163.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(6947..7156)
                     /locus_tag="J1Z28_002336"
     CDS             complement(6947..7156)
                     /locus_tag="J1Z28_002336"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717164.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            7211..11173
                     /gene="putA"
                     /locus_tag="J1Z28_002337"
     CDS             7211..11173
                     /gene="putA"
                     /locus_tag="J1Z28_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA7717165.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAQVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11213..11851)
                     /gene="rutR"
                     /locus_tag="J1Z28_002338"
     CDS             complement(11213..11851)
                     /gene="rutR"
                     /locus_tag="J1Z28_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBA7717166.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            12139..13230
                     /gene="rutA"
                     /locus_tag="J1Z28_002339"
     CDS             12139..13230
                     /gene="rutA"
                     /locus_tag="J1Z28_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBA7717167.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            13230..13922
                     /gene="rutB"
                     /locus_tag="J1Z28_002340"
     CDS             13230..13922
                     /gene="rutB"
                     /locus_tag="J1Z28_002340"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBA7717168.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELMPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            13934..14320
                     /gene="rutC"
                     /locus_tag="J1Z28_002341"
     CDS             13934..14320
                     /gene="rutC"
                     /locus_tag="J1Z28_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBA7717169.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14328..15128
                     /gene="rutD"
                     /locus_tag="J1Z28_002342"
     CDS             14328..15128
                     /gene="rutD"
                     /locus_tag="J1Z28_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBA7717170.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLVQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            15138..15728
                     /locus_tag="J1Z28_002343"
     CDS             15138..15728
                     /locus_tag="J1Z28_002343"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBA7717171.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPTRIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            15739..16233
                     /gene="rutF"
                     /locus_tag="J1Z28_002344"
     CDS             15739..16233
                     /gene="rutF"
                     /locus_tag="J1Z28_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBA7717172.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQESLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            16254..17582
                     /gene="rutG"
                     /locus_tag="J1Z28_002345"
     CDS             16254..17582
                     /gene="rutG"
                     /locus_tag="J1Z28_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBA7717173.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQKP"
     gene            complement(17665..17838)
                     /locus_tag="J1Z28_002346"
     CDS             complement(17665..17838)
                     /locus_tag="J1Z28_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBA7717174.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18211..18807
                     /gene="wrbA"
                     /locus_tag="J1Z28_002347"
     CDS             18211..18807
                     /gene="wrbA"
                     /locus_tag="J1Z28_002347"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBA7717175.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            18828..19055
                     /locus_tag="J1Z28_002348"
     CDS             18828..19055
                     /locus_tag="J1Z28_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717176.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(19093..20334)
                     /gene="agp"
                     /locus_tag="J1Z28_002349"
     CDS             complement(19093..20334)
                     /gene="agp"
                     /locus_tag="J1Z28_002349"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBA7717177.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQVPAQRVTLELSGCP
                     IDANGFCPMDKFDRVLNEAVK"
     gene            20869..21789
                     /gene="cbpA"
                     /locus_tag="J1Z28_002350"
     CDS             20869..21789
                     /gene="cbpA"
                     /locus_tag="J1Z28_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBA7717178.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            21789..22094
                     /gene="cbpM"
                     /locus_tag="J1Z28_002351"
     CDS             21789..22094
                     /gene="cbpM"
                     /locus_tag="J1Z28_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBA7717179.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT         5205 a         5920 c         5740 g         5251 t
ORIGIN      
        1 aatcagggga atgtattcgg tgttaatcag tcagtcaaat tatggtccat tattgatgga
       61 acctcatacg caaaattggc accagacctc ctgtacaggt tgaatggctg aaaaaagata
      121 ggcagatgct cgctttttgg gcttctgaaa gaaaaactct ccgcactggc ggtattacac
      181 tggtctggtg ctatgacgac aaatgcccgg taagagtatc taaacctgaa ttcatttcag
      241 cttatcaaca cgctcaaagt cgccccctga acgttaacct gaagtgaaaa tctttgctgt
      301 taatgaaata aaacaaagcc cggttcgccc gggctctgca ccgataatac acttaactgt
      361 atgcatgtag cgtacgttgg caaagtgccg aacgtacgca atcctcttta ccgaatcgga
      421 cgatcccgac catttcatcc tcttcgaaac gttccagcgc gtcacttaat ccggagcgca
      481 cgccgcgagg caaatcgcat tgcgtgatat caccgttgac gataaccgtc acgttctccc
      541 cgaggcgggt taaaaacatt ttcatttgcg cggcagtcac attctgcgcc tcgtcaagaa
      601 tgacgactgc attttcaaag gtacgtccac gcatataggc gaacggcgca atttccacct
      661 tccctatttc cggtcgcagg cagtactgca taaaggaagc ccctaagcga cggaccagca
      721 cgtcgtagac cgggcgaaaa tagggagcaa acttttctgc gatatcacca ggtaagaagc
      781 caagatcttc atcagcttgc agaactggac gggtgacgat aatcctgtcg acatccttat
      841 gtatcagggc ctctgccgct tttgctgcgc tgatccaggt ttttccgcac ccggcttcgc
      901 ccgtggcaaa tatcagctgc ttactctcaa tagccttcag atagtgcaat tgcgcttcat
      961 ttcgcgcgag gatgggcgag gtatcgcgac tgtcgcgggc cataccaatg gcttctacgc
     1021 cgctcatctg cacaagcgag gtgaccgatt cttcttcacg ctgcttatgg ctgcgcgaat
     1081 cccgtctcag cacacgtttt gcctcgcgac gagctttgat cactgctttt tgtcttccca
     1141 tggagagcac cttgagttgt ttgtattcat cacacgcgcc gttggcagcg cgattatgcg
     1201 cacgaacatc agagggttgg cttccttgta agccatagtt tgcttttgga taaaatgccg
     1261 aaaaacggct acgcgcaccg tttacggcgt cggtaacaca tgaaaagaaa ggatgaggtt
     1321 gaaaatacaa agtgacgaga tgactaccgg aggagaaatc tccgcgagtg gtggcgcgct
     1381 gattatctaa aacatgtcca gtacaggacg ttaccatccg cgatctccat agtgactgac
     1441 tatcactgcc gggaacttcc gctgctactt aataagtaca acagatttcg cattcattgc
     1501 aacaatatat tcacttatat ttaactataa aacaccattt cagtgacatt agtttctacc
     1561 ggaaagatga cagagtgatg acagtgatga aaaaagctgt gtgctttcag caggattcgc
     1621 agtactggta aaagcttgcc ggatgcggct tgaacgcctt atccaaccta ccgacccgca
     1681 ccgattgtag gcatgataag acgcgcaagc gtcgcatcag cgcacgaacg cctggtgcgg
     1741 cgtaaacgcc ttatccgcct aaaaacatta aacccgcaat aatgcgcttc cgagataatc
     1801 gttcgcgtcc ttcacacccg gcagcgcaaa gaaatagccg ccaccgatag gtttgacata
     1861 ttcctccagc gcctcgccat tcaacctttt ttgtactgtc aggaagccct cttccagatc
     1921 gtgttggtag cagacaaaca gtagccccat atcgagttgc ccggaattgg tgacgcccaa
     1981 cgaataactg tagccacgac gcagcatcag gctggactcg ctctccgccg tgcggggatt
     2041 cgccagccgg atatggctgt ccagcgcaat caccttccct tccggatcgc tagcgtaatc
     2101 gggaacatca tgctcatgct gcatgcccag cggcgcaccg gtttgcttat cacgaccaaa
     2161 tatcgtctgc tgctctttca gcggcgttct gtcccaaaat tccactcgaa actgaatcaa
     2221 gcgcactgcc tgatagctgc caccgattgt ccacgcaggc tcctgctgat ctgccgtcac
     2281 ccacaccact ttttgcatca acttcgcatt ctggctatcg ggattggcgg tgccgtcttt
     2341 aaagcccagc aaatttatcg gcgtctcttt gcctttacta cgcgctgcgt ggtcggaaat
     2401 aaacccttcc cgcttccagc gcacgctgag caaatccggc gtgtgtttga tgatatcgcg
     2461 cagcgcatgg atcaccgtgt cctgggtgtt ggcgcaaatt tgtagcaaca catcaccatg
     2521 acataacgcc gcatccagcg agtcgttggg gaaacgcgtc atcttctgca actttttcgg
     2581 catctgtggc gcaaggccaa agcgctcatc aaacaatgag tgacccaccg ataacgtgat
     2641 ggtgagatta tcgggcgcaa tgtagccgcc aagaatgccg gaatcgagtg gtggcaggcg
     2701 cggatttggc gtttctggtg ccgctccgcc ctgagtcaga aaagcaaaac gctgagtcaa
     2761 caagcgaaac aaccgttcaa gatcggcttt atcgctggca agcacatcaa acgccaccag
     2821 catcattgca gcctgttgtg gtgtcaggat ccctgcctga tgctcaccat aaaacggctg
     2881 tttctcattg cgtgcatccg gtgaaagcgt acccggcgca ctttgcgttt tttgtgcatg
     2941 agcgaccgga caacttcccg ccagcgccag cgcaccaatc cctttcagta aacgtcggcg
     3001 tgacggttca ttcacgccgt tttcatcttc atactgcata acgcttagtc cagccccagc
     3061 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggtccttt cagtgcattc
     3121 cggtcagcat cggtcagttt gtcgtaattt tcaaaaccgt ctttagtacg gtatttcgcc
     3181 agaatggtat cgaccttttt gaagttggca tcgacttttg ccaacagttc cggattcgct
     3241 ttttgtagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3301 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3361 acttcctcaa tcagtccggc tgcaccgccg accacttttg aaggagggaa agccagttca
     3421 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct ggtcagcata cttatccatc
     3481 cctttggtgg tgttatcgcc aaacaatgct ttttccagac ggtggaaacc ggtgaatttc
     3541 ggatcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3601 gagaacagtt cagcaatcgg ttcgatgcgc tcatagtgct ggcgcgtcgg cgcatacagt
     3661 gctttcgctt tttcgatatc gcctgcttta atagcgtcag taaaggcttt ggtgtcggtc
     3721 accagttgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3781 cttaacagcg catcactttg cgccgcatcc gccgttgctt cacctttgac gatcaacttc
     3841 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3901 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3961 ttgaggatct cccactccag cgccttctgg ctgtggttct gaataatgaa ctgtgttttc
     4021 ccggcgttaa ccgtaatggt catcggctcg cactgcttat ccgtcacggt cactttgact
     4081 tgcggaatat cggcggcgtt agccataaaa gcagaagaga acagcgcagc cacgctcaac
     4141 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     4201 taactaagca agagtttgcg tcgtatttgc tacacggagc gggacgcagt cgcccctgcg
     4261 cgtggtggca gaacaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4321 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagtgt gccaaacagc
     4381 gagtgagttg agagcaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4441 ccggcttcat gaaatgcgcg aatggcacca gctgccagcc ctgcggcgac gaagagaata
     4501 aacagactgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4561 aggaagccaa gcaccacggc tgtagcaaga ccgagcattg cacccagcgg cggccagatc
     4621 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4681 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4741 ctatcgaccg cctgctccag ttgtactttg acgttgcgcg acactttgcg catccagaaa
     4801 accatccagg taaggatcac tacggcgatc accgccacaa tgccttcaaa cagttcctgt
     4861 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tacccaagcc caggcacaac
     4921 gcagcggcaa gcaacacgcc aatccacatt acaccaatcc atcggcctcg ctgggtacgc
     4981 ttaagatagc tggcaatcaa actgacaatc agcgcggctt caagtccttc gcgcaacata
     5041 atgagaaacg gaacaaacat gccacccacc cttaaacgtt tttctgagtc aatctatgca
     5101 aagaaaagta aatcgcagtg atagcgatta tcattacgat gaagaaaaat acaagtggaa
     5161 tgtagtgata atgatgagaa gatgtaaaaa tattttgaga aaaaacgcag agggcaatca
     5221 ctgattgccc tctgcctgct tcctgttatg acgtgaaatt tccggtaaaa cgccttccct
     5281 tacccttcct gcaaccgtga cggcggagcc gaatgatagt gcgcatcggc ctcggcaaag
     5341 cgtttttgca tcgccgctga cggcgcttta cccagcaaac taaacactac aatcccgata
     5401 ctgccgaaga taaagcccgg aataatttcg tacagaccca gccagccgaa ctgtttccag
     5461 acgataaccg tcagcgcacc gatgatcatc cccgccagcg caccgttgcg cgtcatgcgt
     5521 gaccacatca ccgagaacag caccaccgga ccaaacgctg cgccaaagcc tgcccacgcg
     5581 tagctcacta agcccagcac gcggttttcc gggtttgccg ccagcgcaat cgccaccagc
     5641 gccaccacca gcaccatcac acgccctacc cacaccagct ctttctggct ggcctgttta
     5701 cgcagaaacg ctttgtataa atcttcggta atcgcactgg agcacaccag cagctggcaa
     5761 cttaacgttg acattaccgc cgccagaatc gccgacagca aaatcccggc aatccacggg
     5821 ttaaacagaa tttgcgccag ttcgataaac acgcgttcag cgttctggtt taccgcaccg
     5881 gctaccgccg ggtgctcgtt aaagtaagcg ataccaaaga agccgacagc caccgcccct
     5941 gccaggcaga ggatcatcca ggtcatactg atacgacgcg catggacaat gctgtgatga
     6001 gaatccgccg ccataaaacg cgccagaatg tgcggctgcc cgaagtaacc cagtccccag
     6061 cccatcagcg agataatggc gacaaaattc aaccctttga gcatatccac gttttcaatg
     6121 ctcttttgtt tgattacttc cagcgagtca ccaaagccac cgacactgat aatgacgata
     6181 actggcgtca ggatcagggc aaaaatcatc agactggcct gaacagtgtc agtccagctc
     6241 accgcgagga aaccgccaat aaaggtgtaa aggatcgtcg ccgcagcccc cgcccacaga
     6301 gccgtttcgt agctcatgcc aaaggtactt tcaaacagac gcgcgcctgc cacaatgcct
     6361 gaagcgcaat aaatggtgaa gaacagcaaa ataaccagcg cggagataat gcgcaaaatg
     6421 cggcttttat cttcaaagcg cccggtgaaa taatccggca atgttaaggc gttattgttg
     6481 tattcggtat gcacacgcaa ccgcccggcc accagtttcc agttgatcca cgcgcctaat
     6541 gtcaggccaa tggcgatcca gctttcggaa atcccggaaa gaaaaacagc gcctggcaac
     6601 cccattaaca gccagccgct catatccgac gcacccgccg ataatgccgt cacgaatggc
     6661 ccaagactac gaccgcccag aatatagtcg tcgaagtttt tcgttgatcg ccaggcgata
     6721 aacccaatca atatcatgcc aaagatatag acacaaaatg tcaccaacat cggtgtgcta
     6781 atagccatct aaagtctcca aaaaattatt atcggcaatg tcgaaacttg ccgttatatc
     6841 tgccaccgga acggggtaac agagtttatg ttttaccagg gcgaccgtat cctgccggaa
     6901 gcgctggtta ttcacaatcg atttaacaca ccatttacat taaattttag tgctcagcga
     6961 cactattttt catcaggttg cactctctca cattttttgc ggttgcacct ttcaaaaatg
     7021 ttaactgccg cagagaaaaa gtctgagtta tttttttaat ccctgtcata tcgatttcgt
     7081 ttattaacat ttcattcatt tttaagcttg ctacgcaggt cacatttaac acggttgcac
     7141 aaagttgcaa catcatagat atttcacgat aacgttaagt tgcacctttc agaacaacag
     7201 gagtaatggc atgggaacca ccaccatggg ggttaagctg gacgacgcga cgcgtgagcg
     7261 tattaagtct gccgcgacac gtatcgatcg cacaccacac tggttaatta aacaggcgat
     7321 tttttcttat cttgaacaac tggaaaacag cgatactctc ccggagctac ctgcgctgct
     7381 ttctggcgcg gccaatgaga gcgatgaagc accgactccg gcagaggaac cacaccagcc
     7441 tttcctcgac tttgccgagc aaatattgcc ccagtcggtt tcccgcgccg cgatcaccgc
     7501 ggcctatcgc cgcccggaaa ccgaagcggt ttctatgctg ctggaacaag ctcgcctgcc
     7561 gcagccagtt gctgaacagg cgcacaaact ggcgtatcaa ctggccgata aactgcgtaa
     7621 tcaaaaaaac gccagtggtc gcgcaggtat ggttcagggg ttattgcagg agttttcgtt
     7681 gtcatcgcag gaaggcgtgg cgctgatgtg tctggcggaa gcgttgttgc gtattcccga
     7741 caaagccacc cgtgacgcgt taattcgcga caaaatcagt aacggtaact ggcagtcaca
     7801 cattggtcgt agcccgtccc tgtttgttaa tgccgctacc tgggggctgc tgtttaccgg
     7861 caaactggtt tccacccata acgaagccag cctctcccgc tcgctgaacc gcattatcgg
     7921 taagagcggt gaaccgctga tccgcaaagg tgtggatatg gcgatgcgcc tgatgggcga
     7981 gcagttcgtc acgggcgaaa ccatcgcgga agcgttagcc aatgcccgca aactggaaga
     8041 gaaaggtttc cgttactctt acgatatgct gggcgaagcc gcactgactg ccgcagatgc
     8101 acaagcgtat atggtttcct atcagcaggc gattcacgcc attggtaaag cgtctaacgg
     8161 tcgtggcatc tatgaaggtc cgggcatttc aatcaagctg tcggcgctac atccgcgtta
     8221 tagccgcgcc cagtatgacc gggtaatgga agagctttac ccgcgtctga aatcactcac
     8281 cctgctggcg cgtcagtacg atattggtat taacatcgat gccgaagagg ccgatcgcct
     8341 cgagatctcc ctcgatctgc tggaaaaact ctgtttcgag cctgaactgg caggctggaa
     8401 cggcatcggt tttgttattc aggcttatca aaaacgctgc ccgttggtga tcgattacct
     8461 gattgatctc gccacccgca gccgtcgccg tctgatgatt cgcctggtga aaggcgcgta
     8521 ctgggatagt gaaatcaagc gtgcacagat ggacggcctt gaaggttatc cggtttatac
     8581 ccgcaaggtg tataccgacg tttcttatct tgcctgtgcg aaaaaactgc tggcggtgcc
     8641 aaatctaatc tacccgcagt tcgcgacgca caacgcccat acgctggcgg caatttatca
     8701 actggcgggt caaaactact acccgggtca gtacgagttc cagtgcctgc atggtatggg
     8761 cgagccactg tatgagcagg tcaccgggaa agttgccgac ggcaaactta accgtccgtg
     8821 tcgtatttat gctccggtcg gtacacatga aacgctgctg gcatatctgg tgcgccgcct
     8881 gctggaaaac ggtgctaaca cctcgtttgt taaccgtatt gccgacacct ctttgccact
     8941 ggatgaactg gtcgccgatc cagtcactgc tgtagaaaaa ctggcgcaac aggaagggca
     9001 aactggatta ccgcatccga aaattcccct gccgcgcgat ctttacggtc acgggcgcga
     9061 caactcggca gggctggatc tcgctaacga acaccgtctg gcctcgctct cctctgccct
     9121 gctcaatagt gcactgcaaa aatggcaggc cttgccaatg ctggaacaat cggtcgcggc
     9181 tggtgagatg tcgcccgtta ttaaccctgc ggaaccgaaa gatattgtgg gctatgtgcg
     9241 tgaagccacg ccgcgtgaag tagaacaggc gctggaaagt gcggttaata acgcgccaat
     9301 ctggtttgcc actcctccgg ctgaacgcgc ggcgattttg catcgcgctg ccgtgctaat
     9361 ggaaagccag atgcagcaac tgattggtat tctggtgcgt gaagccggaa aaaccttcag
     9421 taacgccatt gccgaagtgc gcgaagcggt cgattttctc cactactatg ccggacaggt
     9481 gcgggatgat tttgctaacg aaacccaccg tccattaggg cctgtggtat gtatcagtcc
     9541 gtggaacttc ccgctggcta ttttcaccgg acagatcgct gccgcactgg cggcaggtaa
     9601 cagcgtgctg gcaaaaccgg cagaacaaac gccgctgatt gccgcgcaag ggatcgccat
     9661 tttgctggaa gcgggtgtac cgccaggcgt ggtgcaattg ctgccaggtc ggggtgaaac
     9721 cgtgggcgcg caactgacgg gtgatgatcg cgtgcgcggg gtgatgttta ccggttcaac
     9781 cgaagtcgct acgttactgc agcgcaatat cgccagccgc ctggacgctc agggtcgccc
     9841 tattccgctc atcgctgaaa ccggcggcat gaacgcgatg attgtcgatt cttcagcact
     9901 gaccgaacaa gtcgtaatag atgtgctggc ctcggcgttc gacagtgcag gtcagcgttg
     9961 ttcggcactg cgcgtgctgt gcctgcaaga tgagattgcc gaccacacgc tgaaaatgct
    10021 gcgcggcgca atggccgaat gccggatggg taatccgggt cgcctgacca ccgatatcgg
    10081 tccggtgatt gatagcgaag cgaaagccaa tatcgagcgt catattcaga ccatgcgtag
    10141 taaaggccgt caggtgttcc aggcggtgcg ggaaaacagc gaagatgccc gtgaatggca
    10201 aagcggcacc tttgtcgctc cgacgctgat cgaactggat gactttgccg aattacaaaa
    10261 agaggtcttt ggtccggtgc tgcatgtggt gcgttacaac cgtaaccagc tcccagagct
    10321 gatcgagcag attaacgctt ccggctatgg tctgacgctt ggcgttcata cgcgtattga
    10381 tgaaaccatt gcccaggtca ctggctcggc ccaggtcggt aatctgtacg ttaaccgtaa
    10441 tatggtgggc gcggtggttg gggtgcaacc gttcggcggc gaagggttgt ccggtaccgg
    10501 gccgaaagca ggcggtccgc tctatctcta ccgtctgctg gcgaatcgcc cggaaagtgc
    10561 gctggcggtg acgctcgcgc gtcaggatgc ggagtatccg gtcgatgcgc agttgaaagc
    10621 cgcattgact cagccgctaa atgcactgcg ggaatgggcg gcaaaccgtc cagaattgca
    10681 ggcgttatgt acgcaatatg gcgagttggc gcaggcagga acacaacgat tgttgccagg
    10741 gccgacgggt gaacgcaaca cctggacgct gctgccgcgt gagcgcgtgt tgtgtattgc
    10801 ggatgatgaa caggatgcgc tgactcagct cgccgccgtg ctggcggtgg gtagccaggt
    10861 gctgtggccg gatgatgcgc tgcatcgtca gttagtgaag gcattgccat cggcagtcag
    10921 cgaacgtatt caactggcga aagcggaaaa tataaccgct caaccatttg atgcggtgat
    10981 cttccacggt gattcggatc agcttcgcgc attgtgtgaa gccgttgccg cgcgggatgg
    11041 tgcaattgtt tcggtgcagg gttttgcccg tggcgaaagc aatatccttc tggaacggct
    11101 gtatatcgag cgttcgctga gtgtgaatac cgctgctgct ggcggtaacg ccagtttaat
    11161 gacgatcggt taaacaggta gccggaggat gttacaacct cctccggcat ctttaacgtg
    11221 gtcgaatccc ctcaataata atccgctgca cgttttcaac cgtttgattg aaaaacacct
    11281 catcgcgcaa cgtcgcgcct gtcaccgcct ccacctgagg ggcaaaatcg gcgtaatgtt
    11341 gagtggaagc ccaaatcata aaaatcaaat gctgcgggtc aatcggcgcg agtttgccgc
    11401 ttttaaccca accggcaatc agcgccgatt tctcatcaat taatgccttc aaatcgcccg
    11461 tcagttcatc cattaacagc ggtgcacctg ccagcatctc catacagaac aggcgcgaag
    11521 cctgcggata atcgcgggaa acttccagct tcagacggat gtattctttg atcgccgcca
    11581 gcggggcgaa atcttctcga aacgctttta acggtgccag ccagatatcg agaatctgcc
    11641 gcagtacggc tatatacagc gcctcttttg acggaaagta atacagcaga ttggtttttg
    11701 aaacacccgc caactccgcg atctgctcca gccttgtgcc gtgaaaaccg aattgtgaaa
    11761 aagtgtccag tgctgcgcta agaatcgctt ttttcttcgc gcttactgcg cgcgaacgct
    11821 tacccgttgt tttcactgcg ccttgcgtca tgcgctctcc cctcttgttg attggcaaca
    11881 ggatagcaaa acccccgccc ggcctcgacc ttctgcactc tcatcgcgca cgaatgcatg
    11941 ttttatgtgc aactgttttg accgtttagt ccactttttt tcaggcattt tagacaactt
    12001 aacattaaca cattaataac attcactttt taaaaactgg catccgcttt gcaaacaagc
    12061 cattacgcgg ctgaaaagga tatgcaggat gcagcgcccc gtctgacttt cactttacga
    12121 gacgaagaga ggttgatgat gaaaattggc gtattcgtac ctattggcaa caacggctgg
    12181 ctcatttcga cccacgcgcc gcagtacatg ccgacatttg aactgaataa agccattgtg
    12241 caaaaagcgg agcactacca tttcgatttc gccctgtcga tgatcaaact gcgtggcttt
    12301 ggcggcaaaa ctgagttctg ggatcacaac cttgagtcgt tcaccttgat ggcggggctg
    12361 gcagccgtca cctcacgcat tcagatatac gcgacagctg ccaccttaac gttacctccg
    12421 gcaatcgtcg cccgtatggc cgcaaccatc gactccatct ctggcgggcg ttttggcgtc
    12481 aacctcgtga ctggctggca gaagcccgag tatgagcaga tgggtatctg gcctggcgat
    12541 gactatttct cccgtcgtta cgactatctc accgaatatg ttcaggtgct gcgcgacctg
    12601 tggggctcgg ggaaaagcga ttttaaaggc gattttttca ctatggatga ttgtcgcgtc
    12661 agtccgcaac cgagtgtccc catgaaagtg atctgcgccg ggcaaagcga cgctggcatg
    12721 gcgttctccg cccggtatgc cgattttaac ttctgtttcg gcaaaggcgt aaatacaccc
    12781 acggctttcg ccccgaccgc tgcgcggatg aaacaggccg cagagcaaac cggacgcgac
    12841 gttggctctt atgtgttgtt tatggtgatt gccgacgaaa ccgacgatgc cgctcgtgca
    12901 aaatgggaac actacaaagc gggcgcggat gaagaggcgt taagctggct aactgaacaa
    12961 agtcagaaag atacccgctc aggtactgac accaacgtcc gtcagatggc cgatcccact
    13021 tcggcggtaa acatcaatat ggggacgtta gtcggttctt acgccagtgt cgcgcgcatg
    13081 ttagatgaag tcgcaaacgt gcctggtgcc gaaggcgtgc tgttgacctt cgatgatttt
    13141 ctgtcgggaa tcgaaacctt cggcgagcgc attcaaccac tgatgcagtg ccgcgcccat
    13201 ctccctgcgc tgactcagga ggtggcatga tgacgacctt aaccgctcga ccggaagcca
    13261 ttaccttcga tccgcagcaa agtgcgctga tcgtggtgga tatgcaaaac gcttatgcca
    13321 cgccaggcgg ctacttagat ctcgccgggt ttgatgtctc aaccactcgc ccggtcattg
    13381 ccaacattca aaccgccgtg accgcagcgc gaacggcagg gatgctgatc atctggtttc
    13441 aaaatggctg ggatgaacag tatgtcgagg ctggcggccc cggctcacca aattttcata
    13501 aatcgaacgc cctgaaaacc atgcgtaatc agccgcagct gcaggggaaa ttgctggcga
    13561 aaggctcctg ggattatcaa ctggtggatg aactgatgcc gcagcctggc gatattgtgc
    13621 tgccgaagcc gcgctacagc ggtttcttca atacgccgct ggacagcatt ttgcgcagcc
    13681 gtggaatacg ccatctggtt ttcaccggta tcgctaccaa cgtctgcgtc gaatcgacgc
    13741 tacgcgacgg cttttttctg gagtatttcg gcgtggtgct ggaagacgca acacaccagg
    13801 cggggccgga atttgcacag aaagccgcgt tgttcaatat cgaaaccttt tttggctggg
    13861 tcagcgacgt cgaaacgttc tgcgacgcgc tttcttccac gtcctttgct cgtatcgctt
    13921 aaggagttta acgatgccaa aatccgtaat tattcctgct ggcagcagcg caccgctggc
    13981 ccccttcgtt cccggcacgc tggctgatgg cgtggtgtat gtctccggta cgctggcttt
    14041 tgatcaacat aacaacgtgc tgtttgccga tgacccaaag gcgcaaaccc gccacgttct
    14101 ggaaaccatc cgcaaggtca tcgagacggc gggtggcaca atggcggatg tgactttcaa
    14161 cagcatcttt attaccgact ggaaaaatta cgccgcgatt aacgaaatct acgccgagtt
    14221 tttcccgggt gacaaaccgg cgcgattctg cattcagtgc ggactggtaa aacctgacgc
    14281 gctggtggaa atcgccacaa ttgcgcatat cgccaagtga ggccgcgatg aaactttcac
    14341 tctcacctcc cccttatgct gatgcgcccg tagtggtgtt gatttcgggt cttggcggta
    14401 gcggcagtta ctggttaccg caactggcgg tgctggttca ggagtatcag gtagtctgtt
    14461 acgaccagcg cggcaccggc aataatcccg acacgctggc agaagattac agtatcgccc
    14521 agatggcagc ggaactgcat caggcgctgg tagccgcagg gattgagcgt tacgcggtgg
    14581 tcggccatgc gctcggtgcg ctggtgggaa tgcagctggc gctggattat cccgcgtcgg
    14641 taactgtgct ggtcagcgtt aacggctggc tacgaataaa cgcccatacg cgccgctgtt
    14701 ttcaggttcg cgaacagtta ctacatagcg gcggcgcgca ggcatgggtg gaagcgcagc
    14761 cgttgttcct ctatcccgcc gactggatgg cggcccgcgc acctcgcctt gaggcagaag
    14821 acgcgctggc actggcgcat tttcagggca aaaataattt actgcgtcga cttaacgccc
    14881 tcaaacgcgc tgactttagt cgccatgcgg atcgtatccg ctgcccggtg caaatcatct
    14941 gcgccagtga tgatctgctg gtgccatcag catgttccag tgaacttcat gccgctctgc
    15001 ccgatagcca gaaaatggtg atgcgctatg gcggacacgc ctgcaacgtg accgatcccg
    15061 aaacgtttaa tgctctgtta ctcaacgggc ttgccagcct gttacatcac cgtgaagccg
    15121 ccctgtaagg aattgctatg aacgaagccg ttagcccagg tgcgcttagc accctgttca
    15181 ccgatgcccg cactcacaac ggctggcggg agacacccgt cagcgatgag acgttacggg
    15241 agatttatgc cctgatgaaa tgggggccga catctgctaa ctgttctccg acacggatcg
    15301 tgtttatccg cactgcagaa ggaaaagaac gtctgcgccc ggcgctttcc agcggcaatc
    15361 tgcaaaaaac cctgaccgcg cccgtcaccg ctatcgtcgc ctgggacagt gaattttatg
    15421 aacggttacc acagctgttt cctcacggcg atgcccgcag ttggtttacc tccagtccac
    15481 aacttgccga agaaacagcg tttcgcaaca gttccatgca ggcggcctat ctgatcgtcg
    15541 cctgccgtgc gctggggctg gataccggcc cgatgtcggg ctttgaccgt caatacgttg
    15601 acgacgcctt ttttgcgggc agcacgctga agagcaatct gctgattaat atcggctatg
    15661 gcgattccag caaacttttt gcgcgcctgc cacgtctgtc ctttgaagaa gcctgcgggc
    15721 tgttgtaagg agcaaacaat gaacattgtc gatcaacaaa cctttcgcga tgcgatgtcc
    15781 tgtatgggcg cggcggtcaa tatcatcact acggacggtc cagccgggcg cgccgggttc
    15841 accgccagcg ccgtctgcag cgtgaccgac acgccgccca cattactggt gtgcctgaat
    15901 cgtggggcgt ccgtctggcc ggtattcaat gaaaatcgaa cgctgtgtgt aaatacactt
    15961 agcgccgggc aggagtcgct ttcaaacctt tttggcggca aaacgcccat ggaacaccgc
    16021 tttgccgccg cccgctggca gactggtgtg accggatgcc cgcaactgga agaggcactg
    16081 gtttcgtttg actgccgtat cagccaggtg gtgagcgtcg gcacccacga cattttgttt
    16141 tgcgccatcg aagcgattca tcgtcacgcc acaccctacg ggctggtgtg gttcgatcgc
    16201 agttatcacg cgctgatgcg ccctgcttgt taacctcctt aaggagacag ctgatggcaa
    16261 tgttcggttt tcctcactgg cagttaaaat cgacctctac agagagcggc gtggtcgcgc
    16321 cggatgaacg attgccgttt gctcaaacgg cagtaatggg cgttcaacac gcggtggcga
    16381 tgtttggcgc gacggtgtta atgccaattc tgatggggct ggatcccaat ctttccattt
    16441 taatgtcggg gatcggcacg ttactgtttt tctttattac cggcgggcgc gtgcccagtt
    16501 atctcggttc cagcgcggct tttgtcggcg tggtgattgc cgcgacgggc tttaatggtc
    16561 agggaattaa tccgaatatc agcatcgcgc tgggcgggat tatcgcctgt gggctggttt
    16621 ataccgtcat tggcctggtg gtgatgaaaa tcggtacgcg ctggattgaa cgactaatgc
    16681 cgccggttgt gacgggtgcg gtagtgatgg cgattggcct gaacttagcg cctatcgcag
    16741 tgaaaaatgt atcggcttcg gcctttgata gctggatggc ggtaatgacg gtgctgtgta
    16801 ttgggctggt ggccgtattt acccgtggca tgatccagcg attattgatt ctcgtaggat
    16861 taattgttgc ctgtctgctg tatggcgtga tgaccaacgt tttagggctg ggaaaagccg
    16921 tcgattttac tctcgtcagc catgctgcct ggttcggtct gccacatttt tccactcctg
    16981 cttttaatgg tcaggcaatg atgctgattg cgcccgtggc ggtgattctg gtggcggaaa
    17041 acttaggtca tctcaaggcg gtcgccggga tgaccggacg caatatggac ccgtatatgg
    17101 ggcgggcatt cgtaggcgat gggctggcga cgatgctttc tggctctgtc ggcggtagcg
    17161 gcgtaaccac ctacgccgag aatattggtg tgatggccgt gacaaaagtc tactcgacgc
    17221 tggtgtttgt tgccgctgcc gtcatcgcca tgctgttggg cttttcgccg aaatttggcg
    17281 cgctgatcca tactattccg gccgcggtga ttggtggtgc atcaattgtt gtgttcgggc
    17341 tgattgccgt cgcaggcgca cggatctggg tacaaaacag tgtggattta agccagaacg
    17401 gtaatttgat tatggtcgca gtaaccttag tgctgggtgc gggtgatttt gctctcacgc
    17461 tgggcggttt tacgttggga gggatcggta cagcaacttt tggcgcgatt ttactcaatg
    17521 cgttgttaag cagaaggttg gttgacgttc caccgccaga agttgttcat cagaagccat
    17581 aaacagaaaa ccgccggggt aaccggcggt gatggctttt tgacgagcta cagacaaaaa
    17641 tgcatgcgtc attgattagc ccggctagtt gtcgctttta ccgtgactgc tcttgccacc
    17701 ttttttacct gcttcagatg cgcgctgcgg gtcatttttg aaattacccc cgctgtgctg
    17761 tccacctttt ttacctgctt ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc
    17821 gcctcgatgg tttgccatta ctgacctccg tctgggtata gacatcatat tgcatagaat
    17881 taacgcggcg tatggcggct gccatatttt cgctacgtgg gactaagctt agtgaactga
    17941 cgcgggcaga cccgcgtttg gtaatatcct gcaacagctt cttagcgaga atagatatca
    18001 tctccaataa ttatccataa gccgctctta tgattaaaaa aacgcgatga gataaaaatg
    18061 tgtcattttg cgacaaaatt acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg
    18121 tgaaatttgc actctgaaaa ggacgtctta tctttaaata agtggtagcg aatcgctacg
    18181 gaatagagat aacacgagga gtggttagaa atggctaaag ttctggtgct ttattattcc
    18241 atgtacggac atattgaaac gatggcacgc gcagttgctg agggtgcaag caaagtggat
    18301 ggcgcagaag ttgtcgttaa gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa
    18361 gcaggcggta aaacgcaaac tgcaccggtt gcaaccccgc aagaactggc cgattacgac
    18421 gccattattt ttggtacacc tacccgcttt ggtaacatgt ccggtcaaat gcgtaccttc
    18481 ctcgaccaga cgggcggcct gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc
    18541 tttagttcca ccggtactgg cggcggtcag gaacaaacca tcacatccac ctggacgacc
    18601 cttgcgcatc acggcatggt gattgtcccc attggctacg cagcgcagga attatttgac
    18661 gtttcacagg ttcgcggcgg tacgccgtac ggcgcaacca ccatcgcagg cggtgacggt
    18721 tcacgccagc ccagccagga agaactgtct attgctcgtt atcaagggga atatgtcgca
    18781 ggtctggcag ttaaacttaa cggctaatct tcaacaggag gatacgcatg ccaactcaag
    18841 aagcgaaagc tcatcacgtc ggtgaatggg catctctgcg caatacgtcg ccggaaatag
    18901 ccgaggccat tttcgaagtc gccgggtacg acgaaaaaat ggcggaaaag atttgggaag
    18961 aaggtagcga tgaagtgtta gtcaaagcct ttgctaagac cgataaagat tcgctttttt
    19021 ggggcgaaca gaccatcgaa cgtaaaaacg tttaaccagc gactcccccg cttcacgcgg
    19081 gggagttttc tgttatttca ccgcttcatt caatacgcga tcaaacttat ccattgggca
    19141 aaaaccgttg gcatctatcg ggcaaccgct taattccagc gtcacgcgct gcgctggtac
    19201 ctgcagggtt aacgcatcgg cattacgtaa ctgttccgca ctctggtaca catattcaat
    19261 tttcatcagg tcgcgattgg ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc
    19321 aatcggcgtg cgttcgttct ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa
    19381 cagagaggca atgttggagt cgtgcccaac taataccgtg attttcggag cactggtgcg
    19441 atcggtgacc agggctttgt cgatataact gaccagcggt ttcgcaacat tgcgcgccac
    19501 ttccggtgag gtaaacaggc tgtcctggta accgtttttc agcttcgaca acaccttcca
    19561 ctgctggtca gatttgattt ctccccaggc cacctgatcc atcggaaaac cttcgtagta
    19621 ttgcaaagta aacgcatcta ccagcgagtt gccgactttc agcggcccgg aaacgcccgg
    19681 ttcttgttga tacttcgcgc taaaggtatt tttgccatct acaagcgaac attgttgctt
    19741 ctctttacac gcaggagaat ctttatagtt aacgatttgt tccagtagct tgtagctgtc
    19801 ggtaagctgg agtttgctga gctctttctc cattgccgcc accgcctgtt cactgaatgc
    19861 ggcggaatca tcggtgatca ctgggttaaa ggtggggtcc atagtgccca ttttttcctg
    19921 gtgatgaaca ggaatatcac acccagggaa tgcgccagta ataaagaact gcgcggtcgc
    19981 gacggtgcgt tgcagactat tggcataggc ataaacggtg tccggtggcg ggcattcccc
    20041 tgatttcacc atcccctgtt ctgccagcca ttcacgcata taatggccca tatacacttc
    20101 gagcacgccc cctttagtgg tgagctgccc accggggacg tcccattcag gccatttatt
    20161 cggcgtcgac tgctccagca cactgccatt gttcgccagc ggcgcacgta agttatggcg
    20221 gctcatcatg agcacttgct gtagctgata gccttccggt acggtttgtg cctgagcatt
    20281 tgaagcgagt aaaactatcc ctgccacagt tgcggcgatt agcgttttgt tcattcctca
    20341 cacctctttt gttatcaata attaaagagt gtgacagaaa tatgtcattt ttccgggaac
    20401 aggttcgcaa aatggctcgt tgaacgcctg tttttctata ttccccttta aaaacaggca
    20461 agttttgacg caaactatgc ctgattagcc ccggcctcgt ttacgccgct aatgattaca
    20521 tcagtagcta taaagaaaat cagcggcgtt accattacca ataaaaaaag aatgtgagac
    20581 taatagaaat ggtttttagc acatgtcttt atcattaaat tgcataacga tttaaatagt
    20641 cgagttgtgc attcctttcc ttattgttta acttgtaagc atgagccaaa agttcaggag
    20701 tgaggtaaat aaaatatgtt cctcccacct tgaggcgacc tcccctccgc gatgatttca
    20761 ataacatatt ctgtgttggc atatgaaatt ttgaggatta ccctacactt ataggagtta
    20821 ccttacaggg gttccttcaa tttgtgttga tttacgcgag ataacgctat ggaattaaag
    20881 gattattacg ccatcatggg cgtgaaaccg acggacgatc tcaagacaat caagaccgcc
    20941 tatcgtcgac tggcccgcaa ataccatcct gatgtcagca aagaacctga tgccgaagcc
    21001 cgcttcaaag aggtcgctga agcctgggaa gtgttaagtg atgaacaacg tcgcgctgag
    21061 tatgatcaga tgtggcaaca tcgcaacgat ccgcaattta accgtcagtt ccatcatggc
    21121 gacggtcaga gttttaacgc cgaagatttt gacgatatct tctcgtcaat tttcggtcag
    21181 catgcccgcc agagccgtca acgccccgcc acacgcggcc acgatattga aatcgaagtg
    21241 gcggtattcc tcgaagaaac gcttactgag cataagcgta ccatcagcta taacctgccg
    21301 gtttataacg cctttggcat gatcgaacag gaaatcccga aaacgctgaa tgtgaagatc
    21361 ccggcgggcg tcggcaatgg tcaacgcatc cgcctgaaag gccaggggac gccgggcgaa
    21421 aacggcggtc caaatggcga cctgtggctg gtgattcaca ttgcgccaca tccgctgttt
    21481 gatattgtcg gtcaggatct ggaaatagtg gtgccggtta gcccgtggga agcggcgctg
    21541 ggtactaaag tcaccgttcc aacactgaaa gaaagcattt tgctgactat cccgcctggc
    21601 agccaggccg ggcaacgatt gcgcgttaaa ggcaaaggtc tggtgagcaa aaaacagacc
    21661 ggcgatctgt atgcggtact gaaaatcgtg atgccgccga aaccggatga aaacactgcc
    21721 gcgctgtggc agcaactggc agacgcccag tcgtcttttg atccacgtaa agattggggg
    21781 aaagcataat ggctaatgtt acggtgactt ttactattac cgaattttgc ctgcataccg
    21841 gcatctctga agaggagttg aatgaaattg tcggtttggg ggtggttgaa ccgagtgaga
    21901 tccaggagac aacctgggta tttgacgacc atgccgccat tgtggtgcaa cgcgcggtac
    21961 gcctgcgtca tgaactggct ctggactggc cggggatcgc ggtggcgctg acgttaatgg
    22021 atgatattgc gcacctgaag caggaaaacc gcctgctgcg ccagcggctt tcccggtttg
    22081 tggctcaccc gtgagtttgt tgcctgatgc gacgct
//
LOCUS       DADSZD010000069        22100 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.069, whole genome shotgun sequence.
ACCESSION   DADSZD010000069 DADSZD010000000
VERSION     DADSZD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.069"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(111..818)
                     /locus_tag="J1Z28_002352"
     CDS             complement(111..818)
                     /locus_tag="J1Z28_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBA7717180.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            1217..3352
                     /locus_tag="J1Z28_002353"
     CDS             1217..3352
                     /locus_tag="J1Z28_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBA7717181.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVCNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3401..4657)
                     /locus_tag="J1Z28_002354"
     CDS             complement(3401..4657)
                     /locus_tag="J1Z28_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HBA7717182.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4859..5938)
                     /gene="mltC"
                     /locus_tag="J1Z28_002355"
     CDS             complement(4859..5938)
                     /gene="mltC"
                     /locus_tag="J1Z28_002355"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBA7717183.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(6003..6278)
                     /locus_tag="J1Z28_002356"
     CDS             complement(6003..6278)
                     /locus_tag="J1Z28_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBA7717184.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6306..7358)
                     /gene="mutY"
                     /locus_tag="J1Z28_002357"
     CDS             complement(6306..7358)
                     /gene="mutY"
                     /locus_tag="J1Z28_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBA7717185.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7519..8238
                     /gene="trmB"
                     /locus_tag="J1Z28_002358"
     CDS             7519..8238
                     /gene="trmB"
                     /locus_tag="J1Z28_002358"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBA7717186.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLSSAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8238..8564
                     /locus_tag="J1Z28_002359"
     CDS             8238..8564
                     /locus_tag="J1Z28_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF469 domain-containing protein"
                     /protein_id="HBA7717187.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            8748..9467
                     /locus_tag="J1Z28_002360"
     CDS             8748..9467
                     /locus_tag="J1Z28_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBA7717188.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9643..10689
                     /gene="ansB"
                     /locus_tag="J1Z28_002361"
     CDS             9643..10689
                     /gene="ansB"
                     /locus_tag="J1Z28_002361"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBA7717189.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGMLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10806..11813
                     /locus_tag="J1Z28_002362"
     CDS             10806..11813
                     /locus_tag="J1Z28_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBA7717190.1"
                     /translation="MKKQWIVGTALFMLMTGNVWADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11882..13018)
                     /gene="hemW"
                     /locus_tag="J1Z28_002363"
     CDS             complement(11882..13018)
                     /gene="hemW"
                     /locus_tag="J1Z28_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBA7717191.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFSQYTGLS
                     EEVIRPQLEEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(13011..13604)
                     /locus_tag="J1Z28_002364"
     CDS             complement(13011..13604)
                     /locus_tag="J1Z28_002364"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBA7717192.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(13612..13902)
                     /locus_tag="J1Z28_002365"
     CDS             complement(13612..13902)
                     /locus_tag="J1Z28_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBA7717193.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(13899..14465)
                     /gene="yggT"
                     /locus_tag="J1Z28_002366"
     CDS             complement(13899..14465)
                     /gene="yggT"
                     /locus_tag="J1Z28_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBA7717194.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFVKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(14483..15187)
                     /gene="yggS"
                     /locus_tag="J1Z28_002367"
     CDS             complement(14483..15187)
                     /gene="yggS"
                     /locus_tag="J1Z28_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBA7717195.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGITGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            15205..16185
                     /locus_tag="J1Z28_002368"
     CDS             15205..16185
                     /locus_tag="J1Z28_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBA7717196.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVKEGRL"
     gene            complement(16359..16775)
                     /gene="ruvX"
                     /locus_tag="J1Z28_002369"
     CDS             complement(16359..16775)
                     /gene="ruvX"
                     /locus_tag="J1Z28_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBA7717197.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16775..17410)
                     /locus_tag="J1Z28_002370"
     CDS             complement(16775..17410)
                     /locus_tag="J1Z28_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708713.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBA7717198.1"
                     /translation="MTAPFFPRILGAVAFLNQETEPLTMNLQHHFLIAMPALQDPIFR
                     RSVVYICEHNTNGAMGIIVNKPLENLKIEGILEKLKITPEPRDESIRLDKPVMLGGPL
                     AEDRGFILHTPPSNFASSIRISDNTVMTTSRDVLETLGTDKQPSDVLVALGYASWEKG
                     QLEQEILDNAWLTAPADLNILFKTPIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(17447..18397)
                     /gene="gshB"
                     /locus_tag="J1Z28_002371"
     CDS             complement(17447..18397)
                     /gene="gshB"
                     /locus_tag="J1Z28_002371"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBA7717199.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(18410..19141)
                     /gene="rsmE"
                     /locus_tag="J1Z28_002372"
     CDS             complement(18410..19141)
                     /gene="rsmE"
                     /locus_tag="J1Z28_002372"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBA7717200.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(19221..19928)
                     /gene="endA"
                     /locus_tag="J1Z28_002373"
     CDS             complement(19221..19928)
                     /gene="endA"
                     /locus_tag="J1Z28_002373"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBA7717201.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(20023..20520)
                     /locus_tag="J1Z28_002374"
     CDS             complement(20023..20520)
                     /locus_tag="J1Z28_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBA7717202.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(20597..21991)
                     /locus_tag="J1Z28_002375"
     CDS             complement(20597..21991)
                     /locus_tag="J1Z28_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HBA7717203.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         5445 a         5587 c         5819 g         5249 t
ORIGIN      
        1 cgggcaacgg ggcgcattaa acctgattcg cctcgtctcg tcaatcaaat ttcttcaatt
       61 tagtgcagac tgcacaatct atcatcactt tgccgctatt gcacgcattg tcaggcaaac
      121 caggcggtcc aggcggcgga aagcggcatt gccagaagga gtgctggaag catgttgacc
      181 accgggaaca ttttaatgcc acagatgcgt aatccggttg ccagcagcaa taaaccgcct
      241 acggcgctga agtctgccat catcgacggt gtggtcagcg gtaatatcag cgcggcagcc
      301 cacgccagtg ttaactggat gatcagtaat ggaatgctaa tcaccgacac cgcaataccc
      361 agcgagcagg cgaagatcat cgccgtaaag aaatcaagaa atgacttggc gattaaaata
      421 cttggatcgc cggtcatccc ttcgttcatc gccccgaaga ttccggtgcc gctggcgcaa
      481 aacaggacaa taatcgcgac aaaattctga ataaaagatt catgcgctgg cttcttacgc
      541 gagtggtgaa acagattatg cgctttggcg accactgtat tgacgccttt ttccagcaga
      601 caaatttcgc cgattaatgc tccgagtaag gttgctaaaa ccatcgccgg aaggttggcg
      661 catttcacca ccagtaaaat accaatcccc agcgatgcca gaccaaaaat tgatgtcatg
      721 gagacgcgga tacgttccgg taagcgttgg ctaaggagtg cgccgaaaac gccacccagt
      781 aagactgcac ttgcgttgat aaaagggccg atgaccacaa gagttcctgt tagctgtatt
      841 gtttgatttt cttattatga acttttggcg ccgtcgatgg cttgtttgtt cgcaaagtcc
      901 tggcttgcac gctttagcga aaggtgccat gattgcgcga attttctcct ctctgtacgg
      961 agtttgcccg atgcacgcca cctccttaca ttctctcgct tatcgccgtt tcgcgcgaaa
     1021 ctcttctctt tttatgctac tgctcatgcg gtgaagttaa cgcacgctca ctgcaggaca
     1081 acagtaaaat cagagcgttt ctgcttttac tgatgtctgg cggtcggagc tggtgaccag
     1141 tttgacccat atctcatggg gcagggtttt ccaccttgtc cggtattctt acttccccta
     1201 aacgggtttt gcgcttatga aatcaatgaa tattgccgcc agtagtgaac tggtatcccg
     1261 actttctact catcgccgcg tggtggcgtt gggagatact gattttacgg acgtcgcggc
     1321 agtcgtcatt accgctgcgg atagtcgcag tggcattctt gcgttgctta agcgcaccgg
     1381 ttttcatcta ccggtgtttt tgtattccga acatgctgtt gaattacctg cgggcgttac
     1441 ggcggtaatc aacggcaacg agcagcagtg gctggagctg gaatccgcag cctgtcagta
     1501 tgaagagaat ttgctgccac cgttttatga cacgctgacg cagtacgttg agatgggtaa
     1561 tagcaccttt gcttgccctg gacatcaaca tggtgcgttc tttaaaaagc atcctgccgg
     1621 acgccatttt tacgatttct ttggcgagaa catctttcgc gccgatatgt gtaacgctga
     1681 cgtaaaattg ggcgatctgc ttattcatga aggatcggcg aaagatgcgc agaaatttgc
     1741 agccaaagtc tttcatgccg ataaaaccta ttttgtgctg aacggcacat cggcagcgaa
     1801 taaagtggtg acgaatgcgc tgttaacgcg tggcgatctg gtgctcttcg accgtaacaa
     1861 ccataagtcg aatcatcatg gcgcgttgat tcaggcgggg gcgacgccgg tctatctgga
     1921 agcttcacgt aacccgtttg gtttcattgg cggtattgat gcgcactgtt ttaatgaaga
     1981 gtatctgcgc cagcaaattc gcgacgttgc gccagaaaaa gccgacctgc cgcgcccgtt
     2041 tcgcctggcg attattcagc tgggaaccta tgacggcact gtctgtaacg cccgtcaggt
     2101 gatcgatacc gttgggcatc tgtgtgatta cattctgttt gattccgcgt gggtcggtta
     2161 cgaacagttt atcccgatga tggcggatag ctcgccgctg ctgttagaac ttaacgaaaa
     2221 cgatccgggg atctttgtga cccagtcggt gcacaaacag caggcgggat tctcacagac
     2281 gtcgcagatc cataaaaaag ataaccatat ccgtggacag gcgcgttttt gcccgcataa
     2341 gcggttgaat aatgccttta tgctccatgc ttctaccagc ccgttctatc cgctgttcgc
     2401 cgccctggac gttaacgcca aaattcatga aggggagagt gggcgtcggc tgtgggctga
     2461 gtgcgttgag ttgggtattg agtcgcgcaa ggctattctt gcgcgctgta agctgttccg
     2521 cccgtttatc ccgcctgttg ttgatggcaa attgtggcag gattatccga cgtcagtgtt
     2581 agccagcgac cgccgttttt tcagttttga gccgggggcg aagtggcacg gctttgaagg
     2641 atatgccgcg gatcagtatt ttgttgatcc gtgcaagctg ttactcacca cgccgggtat
     2701 cgatgccgaa accggcgaat atagcgattt tggcgttcca gcgacgattc tggcgcacta
     2761 tctgcgtgag aacggcattg tgccggagaa gtgcgatctc aactccattc tgttcttatt
     2821 aactccggcg gaaagccacg agaagctggc acaactggtg gcgatgctgg cgcagtttga
     2881 acagcatatt gaggatgatt cgccgctggc tgaggtgttg ccgagcgttt ataacaaata
     2941 tccggtgcgc tatcgcgact acaccctgcg ccagttgtgt caggagatgc acgatttgta
     3001 tgtcagtttc gacgtcaaag acctacaaaa agcgatgttc cgccagcaga gtttcccgtc
     3061 agtggtgatg aatccccagg atgcgcatag cgcttatatt cgcggtgaag tggagttggt
     3121 gcggattcgt gatgccgaag ggcgaattgc ggcagaaggg gcgttgcctt atccacctgg
     3181 cgtgctttgc gtggtacccg gggaagtctg gggtggggcg gttcaacgtt atttccttgc
     3241 gctggaagaa ggcgtgaatt tgttgccggg attttcgccg gagttgcaag gtgtctatag
     3301 cgaaaccgat gcggatggca tgaaacggtt gtacggttat gtgttgaagt aagaataaaa
     3361 aaacgggtca ccttctggcg acccgttttt ctttgcgtaa ttagtggcta accgtctgtg
     3421 tacctgtcgg gacacgaacg tgtttatatt tgaacatcgc catgaacgcg aaggccagaa
     3481 ccacggagta acctgcgaaa atcagccata cggtctgcca gtcggtaatg ccgttttggg
     3541 tgtacatctc aaccacttta ccgctcacga tgccgccgag gatacagccg aagccgttgg
     3601 tcatcatcag gaacatcccc tgcgcgctgg cgcgaattgc cgggctaact tctttttcga
     3661 caaacaccga accagagatg ttgaagaagt cgaatgcgca accgtaaacg atcatcgaca
     3721 gtaccagcag tacagtaccg aacggagtcg ggtcgccgta agcaaacaga gcaaaacgca
     3781 ggatccacgc cacaatactg atcatcatta cgttcttaat accgtagcgg cttaagaaga
     3841 acgggatggt cagaatgaac agggtttcag agatctgcga aatcgacatg atgattgacg
     3901 catgctgcac gatgaagctg ctggcaaaca tcggatcttt gtcgaagctg tgcaggaagg
     3961 tattaccgaa catgttggta atctgcagtt ccgcgcccag cagcattgag aagatgaaga
     4021 agattgccat acgcttgttt ttaaacagcg cgaatgcatc gaggcccagc agggttgtcc
     4081 agctctgatt cgcttgctgt ttagcaaccg gaatatgcgg cagagtcagg gtaaacagaa
     4141 ccagaatggc ggaaagcgct gcgccaatat acagctgcat gtggcttaat tcgaagccgg
     4201 acaggctcac cacccacatt gccatgataa agccgatggt gccccagata cggattggcg
     4261 ggaagtcagt aacgatatcc atcccggcat tttgcaggcg atagtaagag atggtgttga
     4321 ttaacccaag cgttggcata taagcaaacg agttaatcaa tatcacaagg aacatcgcct
     4381 ccggcgtagt gacctctgcc gccatgaaca gcgtgatagc gccaatggtg tggcaaatgg
     4441 catagaccca tttcgcactt aaccatttgt cggccacaat ccccagcagc gcaggcataa
     4501 agaccgcggc gatacccagt gagctataaa ctgcgccgat agaagcaccg tcaaacttca
     4561 gggtaacaaa catataggag ccgagggtcg tcagccaact tccccacaga cagaactgca
     4621 gaaaagagag gattttcagc tgcagcttaa gattcatgtt aatttcctca catcgtgatg
     4681 cggatgagtg tttgaaaaag cacatttgac cgtgcgagat gatgcgattc tatcaacttc
     4741 gcggactggt tttttgttac ctgtggcaaa taattgcaaa cattcccaat ttgcaaaacg
     4801 aaattgtgat gtagataaca tttttacccc tgagcatcgt caggggcggt tagtggaatt
     4861 atcggcggcg gtaagatttt tgcgcggtat tcactttata aagataacgg cgagattccg
     4921 cagagggatg gcgggtcgtc agcgtctgat aaacatcgcc cggcgtcatg gtgttaataa
     4981 tattggcagc ctgaatctta tcattcgaaa agactcgcag cacgctgcct gcgccgccgt
     5041 tataggcggt gatgacggca taacgccgcg atgttgggtt atcaattccg ccgagataaa
     5101 cgttgttcag catcgccaga tacgcggtgc cggtatcaat attgctggca ggatcaaaca
     5161 agaaactgcg gctcggcgtg ccggatttcc cctgcgagcg gaacacatct ttcccggcag
     5221 tatgttgtac cacctgcatt aatcccagcg catcggaacg gctgaccgca tacgggttaa
     5281 aggaagattc agtctgcata attgccagaa ttagcgactc atcaacgcca tatttgcgtg
     5341 acgcctggcg gaccatgccg agatatttgt gcgcacgttt atcaaggtgg ttcggcacca
     5401 tgttaatggt gacgctgtag atgatacgca gcccgttgct gcggctcttc agacggtttt
     5461 tcagcagata atccgcgaag ttgctcgcgc gaccttccca gcgaatcggc tgcccggtgt
     5521 tgtccaccac ctgaccgtaa aggaaaggtt ctttcgaaat cgtaatatca tcaacgtcgg
     5581 aatagagatc gaccgaactc ggatcgtccc ccatcagcaa cgttttgata attgcccggc
     5641 gcagatgcgc ggcaggttct gtcccggcga tggtttcgat agtaatcgta ccgtcatcga
     5701 agttgatgtg gctgcgggtc tgatattgat cggtgtattt cacgtagtcc ttaggaccgg
     5761 cgatcaccac ctctttgaag ccccagatgt tctcaatatt gtgggcaaat tgccccatca
     5821 gaatatcaaa accgttggta tctttgaccc aggcttcgtt ataggtatcg ccttttttgg
     5881 tcgtcgaaca ggagatgagc aacggcgcaa tcaaagccag cgcgagatat tttttcatca
     5941 ttccgggtgc gtgttgtgtt tgtttttatg caagttgccg gaggcgtgct ccggcactgt
     6001 ttttattttt tatcttccgg cgtatagccc tcgatatgca cctctttacc ctcgaacagg
     6061 aagttgacca tctcctgctc cagcagcttg cggtgctcgg cattcatcat gttgagtttc
     6121 ttttcattaa tcagcatggt ttgtttgtgc tgccactgcg cccaggcttc tttggagatc
     6181 tcgttataga tgcgttttcc cagttcgccg gggtacagct gaaaatcctg accttctgct
     6241 tcacgttgca ggaaagtaca aaaaatcgtt ctgctcataa atcatcctct ttatcgactc
     6301 acgcgctaaa ccggcgcgcc agtgcgtaac tgctgtaaca aacgctccac gggagccgcc
     6361 aggccaactg acggcggttg cgctaagtta taccagagcg cattgtcttc atccatgcag
     6421 ccggtgaatg acgacacggg aagccacata ggcacaatat ctaagtggaa atggctgaag
     6481 gtatgccgaa acgcggtcag ctgcgtcagg ttatcggcag caatctgtcg ttgcgccagc
     6541 cactgccgca aactttcttc atcggcaaac tgcgggaaac agtataaacc gccccacaat
     6601 ccactcggcg gacgctgcgc cagcaatact tcatcttcgt gctgtaacag caaaaagtag
     6661 ccggtgcgct ctggcagcgt ctgtttcggt tttttgcccg gataaagcga ccagctattg
     6721 ttggcggcgg caatacatcc gttttgtagc ggacagagcg aacatttcgg cttagagcgc
     6781 gtacaaatca tcgcgcccaa atccatcatc gcctgattaa accgttccac gccaaccgcg
     6841 ggcgtcacct gctcgctcaa actccacagt ttattctcga cctctttttt cccaggccag
     6901 ccgcttacag catagcagcg cgccagcacg cgtttgacgt taccgtcgag aatcggaaag
     6961 tgcttaccca gagaaagcga gagaatcgcg cctgcggtgg aacgcccgac gcccggcagt
     7021 gctgcaactt cctcaaaggt ttccgggaat ttaccgccgt gtaaggccgc cacttgttgt
     7081 gccgctttat gtaggttgcg cgcgcgggcg taatagccaa gcccggtcca caagtggaga
     7141 acttcgtcga gcggcgcatt ggcgagatcg gtcaccgttg ggaagcgcgc cataaagcgt
     7201 tcaaaatagg ggataacggt cgcaacctga gtttgttgca acatcacttc tgagagccat
     7261 actttgtagg gcgtcttgtc aatttgccag ggcagcgttt tccgcccgta tttatcgtac
     7321 cagtccagaa cctgggctga aaattgcgac gcttgcatgg tcaccgaatt cactgttgtt
     7381 gggggcaaga ttgcagcaca gcgacggcag ggtgtaaacc ggaactttcc gcagccacgg
     7441 ccttatcatg cttgcatccg gcaactaact ttggataatg cccgttttca gaacactttc
     7501 acaagcgact aaatctttat gaaaaacgac gtcatctcac cggaatttga tgaaaacggc
     7561 cgcccgctgc gccgtatccg tagttttgtg cgccgccagg ggcgactgac caaaggccag
     7621 gaacatgcgc tggaaaacta ctggccggtg atgggcgttg agttcagcga agatatgctg
     7681 gatttccctg cgctttttgg ccgtgaagcg ccggtgacgc ttgagattgg ttttggcatg
     7741 ggcgcgtcgc tggtggcaat ggctaaagat cgccccgagc aggacttcct cggcattgaa
     7801 gtgcattcac cgggcgttgg tgcgtgcctg tcttctgcgc atgaagaggg cttaagcaac
     7861 ctgcgcgtga tgtgtcacga tgcggttgaa gtgctgcata aaatgattcc tgacaattca
     7921 ttgcgcatgg tgcagctctt tttccctgac ccgtggcaca aagcgcgcca taataaacgc
     7981 cgtatcgttc aggtgccgtt tgccgagctg gtaaaaagca aactgcagct ggggggcata
     8041 ttccatatgg cgaccgactg ggaaccttat gcggaacata tgcttgaagt gatgtcttct
     8101 attgacggtt ataaaaacct gtcagagagc aatgattacg taccgcgtcc ggcatcacgt
     8161 ccggtgacga aatttgaaca acgtggtcat cgtcttggtc acggagtatg ggacttaatg
     8221 ttcgagaggg tgaaataatg gcaaagaacc gtagccgtcg tctgcgtaaa aaaatgcaca
     8281 tcgacgaatt ccaggaatta ggattttcgg tggcatggcg attcccggaa ggtacatcgg
     8341 aagaacagat tgataaaacc gttgatgact ttattaacga ggttatcgaa ccgaacaaac
     8401 tggcctttga cggcagcggt tatctggcct gggaaggtct gatctgcatg caggaaatcg
     8461 gcaaatgcac cgaagaacat caggcgattg tgcgtaagtg gctggaagaa cgcaaactgg
     8521 aagaggtacg caccagcgaa cttttcgacg tttggtggga ctaagaaagc atacgggcga
     8581 tgacaaatgc aaaactgcct gatgtgctac gcttatcagg cctacaaaga tgcacgatcg
     8641 agtaggccgg ataaggcgtt tacgccgcat ccggcatgga aaacgcgcac tttgttatca
     8701 atctggggcc agcaaatgct ggcctgattt gttcttgagg gaagactatg atgcgcaaaa
     8761 tgctgctggc ggcagcactt tcagtgacgg caatgaccgc tcacgccgac taccagtgca
     8821 gcgtcacgcc gcgtgacgat gtgattgtca gcccgcaaac cgtgcaggtg aagggcgaaa
     8881 acggcaatct ggtgatcacg ccagacggca acgtgatgta taacggtaag caatattccc
     8941 tgaatgccgc ccagcgcgag caggcgaagg attatcaggc tgaactacgt agcaccctgc
     9001 cgtggattga tgaaggcgcg aaaagccgcg tcgagaaagc tcgtattgcg ctggataaaa
     9061 ttatcgttca ggagatgggc gaaagcagca aaatgcgcag ccgtctgacc aaacttgatg
     9121 cgcagctgaa agagcagatg aaccgcatta tcgaaacgcg cagcgatggc ctgacgtttc
     9181 actataaagc cattgatcag gttcgtgccg aaggccagca attagtgaat caggcaatgg
     9241 gcggaatttt acaggacagc attaatgaaa tgggcgcgaa agcggtgctg aaaagcggcg
     9301 gtaacccatt acagaacgtg ctgggaagcc tgggcggcct gcaatcctca atccaaaccg
     9361 agtggaaaaa gcaggaaaaa gatttccagc agtttggcaa agatgtttgt agccgcgttg
     9421 tgactctgga agatagccgc aaagccctgg tcgggaattt aaaataatcc tctattttaa
     9481 gacggcataa tactttttta tgccgtttaa ttcttcgttt tgttacctgc ctctaacttt
     9541 gtagatctcc aaaatatatt cacgttgtaa attgtttaac gtcaaatttc ccatgcaaag
     9601 ctaagggata atgcgtagcg ttcacgtaac tggaggaatg aaatggagtt tttcaaaaag
     9661 acggcacttg ccgcactggt tatgggtttt agtggtgcag cattggcatt acccaatatc
     9721 accattttag caaccggcgg gaccattgcc ggtggtggtg actccgcaac caaatctaac
     9781 tacacagcgg gtaaagttgg cgtagaaaat ctggttaatg cggtgccgca actgaaggac
     9841 attgcgaacg ttaaaggcga gcaggtagtg aatattggct cccaggacat gaacgatgat
     9901 gtctggctga cactggcgaa aaaaattaac accgactgcg ataaaactga cggcttcgtc
     9961 attacccacg gtaccgacac gatggaagaa accgcttact tcctcgacct gacggtgaaa
    10021 tgcgacaaac cggtggtgat ggtcggtgca atgcgcccgt ccacgtctat gagcgcagac
    10081 ggtccattca acctgtataa cgcggtagtg accgcagctg ataaagcctc cgctaatcgt
    10141 ggcgtactgg tagtgatgaa cgacaccgtg cttgatggcc gtgatgtcac caaaaccaac
    10201 accaccgatg tagcgacctt caagtctgtt aactacggtc ctctgggtta cattcacaac
    10261 ggtaagattg actaccaacg taccccggca cgtaagcaca ccagcgacac gccgttcgat
    10321 gtctctaagc tgaatgaact gccgaaagtc ggcattgttt ataactacgc taacgcatcc
    10381 gatcttccgg ctaaagcact ggtagatgcg ggctatgatg gcatcgttag cgctggcgtg
    10441 ggtaacggca acctgtataa aaccgtattt gacacccttg caaccgctgc gaaaaacggc
    10501 actgcagtag tgcgttcttc ccgcgtaccg acgggcgcta ccactcagga tgccgaagtg
    10561 gatgatgcga aatacggctt cgtcgcctcc ggcatgttga acccgcaaaa agcgcgcgtt
    10621 ctgctgcaac tggctctgac gcaaactaaa gatccgcagc agatccagca gatcttcaat
    10681 cagtactaat cacttcgccc cggtatcgtg ccggggctta ttcacttcag actcacgtcc
    10741 attgccaatt tttattaccc taatgataat caccggaata aattattccg cgcgagggtt
    10801 ttcgggtgaa aaagcaatgg attgttggta cggcgctgtt tatgttgatg actggtaatg
    10861 tctgggcaga tggtgaaccg ccaactgaaa atatcttaaa agatcaattc aaaaagcagt
    10921 atcacggcat tctcaagctt gatgtcatca ccttaaaaaa tcttgatgct aagggtaatc
    10981 aggccacctg gtcagcggaa ggcgatgtct cttccagtga cgatctctat acctgggtcg
    11041 gtcagttggc agattacgag ctgctcgaac agacctggac gaaagataaa ccggtgaaat
    11101 tctcggcgat gttaaccagt aaaggaacgc ccgcgtctgg ctggtcggtg aacttttact
    11161 cttttcaggc ggcagccagc gatcgtgggc gggtggttga cgatatcaaa acgaataata
    11221 aatatctgat cgtgaatagc gaagatttca attatcgctt tagtcagctt gagtctgcgt
    11281 tgaataacca gaataactcg attcctgcac tgaaaaagga tgtaaaagcg cttgataagc
    11341 aaatggtggc ggcgcagaaa gcagcggatg catattgggg aaaagatgca aacggtaagc
    11401 aaatgacccg cgaagacgcc tttaaaaaaa ttcaccaaca gcgtgatgat tttaataaac
    11461 agaacgatag tgaggcgttc gcggttaaat atgacaaaga ggtttatcaa ccggcgattg
    11521 cggcatgtca taaacagagt gaagagtgtt atgaagtgcc aattcagcag aaacgagatt
    11581 ttgatatcaa cgaacaacgg cgacagactt ttctgcaatc acaaaaactt agccgtaaat
    11641 tgcaggatga ctggataacg ctcgaaaaag ggcaatatcc gttaacgatg aaagtttcag
    11701 aaataaacag taaaaaagtc gcgattttga tgaaaattga cgatatcaac caggctaatg
    11761 aacgctggaa aaaagatacc gaacaactgc ggcgcaatgg ggtgattaag taatttttgc
    11821 gatgacgcat gagttgttgc cggatgctgc gtacacgcac gccgcatccg gcaatacaaa
    11881 tttactccgc cagaaaaagc tccagcaacg aatttaaaaa caacttccca tgttccgtta
    11941 tctgccagta atctgcacat tctgtgagat aaccctgagc gatagcctct tctaactgtg
    12001 ggcgaataac ctcttctgaa aggccagtat actggctaaa ctccacgcgc ggcgcggctt
    12061 ccagcaaacg gaagcgattc ataaagaact caaatggttt atctgcggct tcgacatcac
    12121 gctggctttc cagataccgc ccctgcataa aaccacgcgg atgacgcgtt ttggtggtac
    12181 gcagaatgcg cccatccggg aaggtcactt tgccatgcgc gccgcagcca ataccgatgt
    12241 agtcaccaaa gcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcataag
    12301 cggaagtttc atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa
    12361 tgtcccacag cgcgtcatcg tccggcagta caggagggcg cgagccaaac agcgtattgg
    12421 gttcgatggt cagttgatac caggaaagat gcggcggatt cagttcaata gcctggcgca
    12481 gatcgcctag cgcctcttcc agtgattgat ccggcagccc atgcatcaaa tcgaggttaa
    12541 agctacgtaa ccctaaaccg ctcgccagat ttgccgctcg tttcgcttct tgcgggccat
    12601 gaatgcgccc aagtcgtttc agcttttctt cgctaaaact ctgtacgcca atggagatgc
    12661 ggttcacacc agcacgctga taatcaacaa agcgatcggc ttcaaccgta ccaggattcg
    12721 cttccatcgt aatttctgca tccgctgcca gcggcaaacg cgcacgtacg ccgtccagca
    12781 gcgtttgcat cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct
    12841 ttacttcacg gccctgagcg taagccacat cgttgtccag atcgttaagc agatgctgaa
    12901 cataatcgtc gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc
    12961 atttctgcac gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta
    13021 cgtaaagcgt ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct
    13081 tcgcgggtca gttcggcagc ggttttccct tcggaaggta cgaagaagat tggatcataa
    13141 ccaaagccac cagtgcccgc tggttcacga gtaatcacgc ctggccagct accgtggcat
    13201 accagcggag tgggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgcgcc
    13261 tgacgttggt cgtccggtac gtctttcatt gtttccagca gtttttgcag attcttttga
    13321 tcggtcgcgt cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca
    13381 tctaccgcca gaccggagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg
    13441 cgcgctttca gaatcgcgtt ttcgataaag gtcaggccgg tttcttcagc ggaatctacg
    13501 ccgaggtctg tttgggccac gatatcaaga ccgaagtcgc taagcagcga cgccagctca
    13561 cgcactttac cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat
    13621 taacgccgcg atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg
    13681 gccaagttcg cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag
    13741 aaacttcacc agatggctgt tggcctggcc gtcaaccggc ggcgcggtaa tggcgacttt
    13801 tacctcgtcg ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag
    13861 ccgtaaaacc agaccgtcat cattaactgt tacggcactc ataacgccat ccacagcccc
    13921 ggcagcagca tatttccggt cgcctgcaag acttctgcga cacccatatt gatgacatac
    13981 agcagcagaa cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg
    14041 cgaatcgggc gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga
    14101 ccctggctta cccagctcat aatcgccatc accagcagga cccagaatat cagcaggccg
    14161 atggttttca gcagaatcaa taaaccggca atccaaatga ttggcaggaa ggtcaccact
    14221 ttaaacagca cgatggcttt aacgaaactg agaatatagg caaccagcag cgaggcgcta
    14281 tcaatgggtc ccattgccgg aataacgcgg cgcagtggcc cgataatcgg ctgcgttacc
    14341 ttcactacaa actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg
    14401 cgtaataaca gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta
    14461 ttcatggcgt tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag
    14521 tgccgatacg aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc
    14581 ccagagagag cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca
    14641 tttggcgtgc aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca
    14701 gcccgcgcag acgtaaacgc ggtagttcag cgaccgcagc tgccagctcg tccagttcag
    14761 ccagttgaat cccggactta ctgttttcat cactaatgtt aatttgaatc agtacgttaa
    14821 gagggggaag ttctgccggg cgctggtcgt tcagacgggt agcgatgcgc aaacggtcga
    14881 tggtatgaca ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc
    14941 caataaaatg ccattctaat cctgttatgc ccagttcctg aaagtggcga attttatcta
    15001 ccccttcctg aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga
    15061 tggcgctcgc aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc
    15121 cgcaacgcgt tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat
    15181 cgttcatttt ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag
    15241 cataacgtct cggatctaca cctgtgcagc gcctggcccg cacgatggcg tattcgcggg
    15301 agaatggaag ctgcgccgtt tgacgcgccg gacgtcgaag agctactgcg ggagtggctg
    15361 gatgacgatc agcgggcaat attgctggag aatggtcagc tggattttgc tgtgtcgctg
    15421 gcggaaaacc agcgattgcg cggcagtgcg ttcgcacaac ggcaaggtat ttctctggcg
    15481 ttacggctgt taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc accggtattg
    15541 ccggaattac tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc
    15601 aaatctacca cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt
    15661 ctgacgctgg aagatcctgt ggaatatctc tataccagtc agcgatgttt gatccaacag
    15721 cgggagattg gtttgcactg tatgactttc gcatcgggat tgcgggctgc attgcgggaa
    15781 gatcctgatg tgattttgct cggagagctg cgtgatagcg agacaatccg tctggcgctg
    15841 acggcggcag aaaccgggca tctggtgctg gcgacattac acacgcgcgg cgcagcgcag
    15901 gcagttgagc gactggtgga ttcgtttccg gcgcaggaaa aagatcccgt gcgtaatcaa
    15961 ctggcaggta gtttacgggc ggtgttgtca caaaagctgg aagtggataa acaggaagga
    16021 cgcgtggcgc tgtttgaatt actgattaac acacccgcgg tggggaattt gattcgtgaa
    16081 gggaaaaccc accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg
    16141 tttcagcaga gttatcagca gcgggtgaaa gaagggcgct tgtgacgttg ctgtgtctga
    16201 tacgaattgt aaatatgccc gatgcgcaag cttatcgggc ctacaaggga agcgtaactt
    16261 tgaattcgca ctcactgtag gttggataag gcgtttacgc cgcatccaac atgaaacaac
    16321 gagcacattg tcagaaatct gtgcgctagt aaatcgcctt aatatccctg ctcgaaatag
    16381 ctttcgagaa taataaccgc agaggcagag tcgactttgc ctttgttgag cgcccgatag
    16441 ccgccctgtt caaacagacc ggaacgtgcc tccacggtgc taagacgctc gtcatggagc
    16501 tttacttcaa caccgaaacg accatgaata cggttggcaa atttacgcgc tctggcggtt
    16561 aacggctgtt cggtgccgtc catattcagc ggcaaaccga cgatgatttc gtccggctgc
    16621 cactctttca gtaaacgctc gataatgttc cagtccggcg taccgtcctg cgctttaatt
    16681 gcaggcaaag ggcgagcggt gccggtaatg cgttggccga ccgctacgcc aatgcttttg
    16741 gtgccgaagt cgaaggcgag taaggttcca ctcatcaggc gtgtcctgcc acaccaggca
    16801 tggtgagaat atccacacca atcagttttg ccgcctcgcg ccagcggtcg gcaatcggcg
    16861 ttttgaacag aatattcaga tctgccgggg ccgttagcca cgcgttatcg agaatttctt
    16921 gttccagttg acctttctcc caggaggcat aacccagagc caccaatacg tcagacggtt
    16981 gtttatcggt gccgagcgtt tccagcacat cgcgggaagt ggtcatcact gtgttgtctg
    17041 aaatgcgaat gctggaagca aaattggagg gcggagtatg caaaataaac ccgcgatctt
    17101 cagccagcgg accgccgagc ataaccggtt tatccagacg gattgattca tcacgcggct
    17161 ccggcgtgat cttcagcttt tccagaatcc cttcaatttt gagattttcc agcggcttgt
    17221 tgacgatgat ccccattgca ccattggtat tatgttcgca aatgtagacc acggaacgac
    17281 ggaaaatcgg atcctggaga gcaggcatgg caataagaaa gtgatgctgt aaattcattg
    17341 tcagaggttc tgtttcctgg ttcaaaaaag caacagcgcc cagtatgcgt ggaaagaagg
    17401 gcgctgtcac tcagagtctc aacgagatcc ttctcgctaa ggtgggttac tgctgctgta
    17461 aacgtgcttc gatggcatcc attaacattc cggtgatcga caccggaaac tctgcttcaa
    17521 tctcacgaat acaggttggg ctggtgacgt taatttcagt cagacggtcg ccgatgatat
    17581 ccagaccaac aaaaatcagc cctttttctt tcagcgtcgg cccaatctga cgggcgattt
    17641 tccagtcact ttccgtcagc ggacgaggtt caccgcgacc accggcagcc agattgccac
    17701 gggtttcgcc cccctgcgga atacgcgcca ggcaatacgg caccggctcg ccatccacta
    17761 ccagcacgcg tttgtcgcca tctttaatgg ctggcaggta attttgcgcc atgcagtagc
    17821 gagtgccatg ctcagtcagg gtttcggcaa tcacgccgag gtttggatca ccttctttca
    17881 cgcggaaaat cgacgcgccg cccataccgt ccagcggctt aagaatgatg tcgctgtgtt
    17941 tttcccagaa agcttttagc tgcgctttat tgcgtgtgac cagcgtttct ggtgttaagt
    18001 cagagaacca ggcagtgaac agtttttcgt tacaatcgcg caggctctgc ggcttgttaa
    18061 cgatcagcgt ccctttctct tcggcacgtt ccagaatata ggtcgcgtag ataaactcgg
    18121 tatcaaacgg cgggtcttta cgcatcagga tcacatcgag atcggccagc ggcagatcct
    18181 gctcacccgt gaactcgtac catttatcgt agttctgttc gacgctcaaa gtacgggtac
    18241 gagcgcgagc ctcaccattg atcaaataga gatcggccat ctccatatag tgaagttcgt
    18301 aaccacgacg ctgtgcttcc agcaacatag cgaaactgga atctttcttg atgttgatgt
    18361 ttgcgatggg gtccatcacg atgccgagct tgatcattat tcttctccgt tagcccaaat
    18421 cgccaaaacg cacttgtagc gcggtaatgg cggtgagcgc agttgtctca gtacgcaaaa
    18481 cgcgaggtcc caacaggata tcagtaaatt gatagcgggc agtcatggca atttcatctg
    18541 ccgataaacc gccttccggg ccaatcagca ggcggacgcg ttcgaccggt aacggcaacg
    18601 tattgatgct gttactggcg cgcgggtgaa gattcagttt cagtccttca tcctgctctg
    18661 cacaccaggc ttccagatcc atcgctggac ggatttccgg cacccggtta cggccacact
    18721 gctcacaggc agcaattgca atcttctgcc actgctggag cttcttgttc agacgttcac
    18781 tatccagttt aacgccgcag cgctcagaaa aaagtggcgt aatgaggctt acaccgagtt
    18841 cgatcgattt ctggatagta aattccattt tttcaccacg cgacatcacc tgaccgaggt
    18901 gaatatgcag cggagattcg cgatcgtcga tctggccttc cagcaccttc acttccacgc
    18961 tttttttgct ggcgctggta atttcggcgt caaagacctg gttgctgccg tcaaacaatt
    19021 gcagcgcctg tcccggcccc atacgcagta cgcgcccgat atggttggcg gcatcttcgc
    19081 aaagcgcgat gtgagaatgg ctggtcagtg gttcaggatg ataaatgcgg gggatacgca
    19141 tagttaaaaa tccgcgtcgt ctccccacgc ggttgtacgc gtggggtagg ggttaacaaa
    19201 aagaatcccg ctagtgtagg ttagctcttt cgcgcctggc aagcgcgttg cacatacggg
    19261 ttatgattgc cctgcacctt cgcgatgcgt tcatcacgct cgcactccca gtcggtaact
    19321 ggatacatct tgtcccatgc gttgaacagt tgcgtttgct ggcgagagag tgtcaggttg
    19381 tattggtcac gcatatagaa gtaggtgcgc gcaatggcac cgcgtgcacg cgctggtggt
    19441 tcggcaactt tttctttgaa atcgaccttc atggcgcatt gaccgtactg gccttcaccg
    19501 ccattccact ggctgtacat aaagttgccg cgatcgccat tcacctcacc gactgacggc
    19561 tgcaggttat gcatatcgct ttccatcttg cgatagaccg gatctttggc gcaattttta
    19621 cgtccaccgt cctgccagca ctggcgctgg tgaccgaatt gccaggcagg aacgacgtgt
    19681 tcccactcta cgcggctggc acggttttca ttttttcgca cctgatagcc gcacgattgc
    19741 agatcaacaa cgcctttttt gccctgccag ttaattttac atccgcaata aaacgtaccg
    19801 ggcgcgtcag cgtggacttt taccgccgcg gctttcgcct gagaaaaact attgatacct
    19861 tcggccaatg ccgggccgga aaatgctgcg ctcagtacca ccgcagcaat agacaaataa
    19921 cggtacatca cttactccgt gttaaaacga gccagcaacg tagcgaaagt tgttttggta
    19981 tgcaatcagc cagatcagga aagttcctga tagttcaggt ggttatttcg caaccagctg
    20041 ttcgccgcag tgaacacagc gatagacggc ttcgccacga actacgcgat tatgacggcg
    20101 tacggtaagc tgatgctcct ggcacttgca gcggtagggg aaggtgttgc ggcgcacgga
    20161 ttgcagttcg aactgatgcg tacggcgggc gggaacgccc agcacgcttt ccatcatcca
    20221 cttccactct ttgccatgtg gcgctacgcg gccgaaatgt ttccatacca gcaaatgtgc
    20281 cagttcgtgc ggcaccactt cttcaataaa ggcttcactg ttttccaaca gcaaaacggg
    20341 attgaggcga atttcatagc tttccagcca ggctgttccg gcagaggttc cgcgctgggt
    20401 gtaagagagt tttggctccg ggtagttacg ccctagcttc aggttcgcct gggcgagttt
    20461 ttcccgcagg cgacgcataa cggcctgttg gatggcgata gggagacggg atgttttcat
    20521 agcggcagag gatagagcga agaaaagatg actgcaagag gtggcttcct ccatgacggg
    20581 aggaagcctg aggagattaa tcgtgagcgc cgatttcgcg cagtttacga cctttcatca
    20641 gattacgttc aatatgttcc agcgaaacgt gtttggtttc cggtaccagc cacaatgtca
    20701 gcaggataaa cagtacgttc agagcagcat acacccagaa ggtgttggcg ttacccagcg
    20761 tgttgagcat ggtcaggaac gttgcgccaa cgatcatgtt ggcaatccag ttggtggcag
    20821 tggagcaggt gatgccaaaa tcgcggcctt tcagcggctg aatttcggag cacagtaccc
    20881 aaatcagcgg accggcactc atggcaaaac cgacaataaa catcagcagc atggcgatgg
    20941 cgaaatactg cgccgacgga gagtggatac cgatatgcat cattgtaccg agtacgccca
    21001 taccagcagc catcaccagg aagcccagcg ttagcgttgg tttacgtccc cagcggtcaa
    21061 caaggccgat tgcgataaag gtggcaagta cgttggtcag gccgacaatc actgtccccc
    21121 acatttgctc ggtggtgttg gtataacccg ccagttcgaa gattttcggc gcgtaataca
    21181 tgatgacgtt catcccggtg aattgctgca ttacctgcaa tagtacgcca aggaacaccg
    21241 cgcggcggaa gttgctattc tctttaaaca gcgcccagcc actctgttta acctgcaaac
    21301 tttcacggat ttcatccagt tcgcgtttcg cttccgcgct ggtgtcacgc aggcgtagca
    21361 gcacgcgttc ggcatcaaca aaacggcgtt tggcggcaaa ccaacgtggg ctgtctggca
    21421 ggaagaagac accaatcagc agcaaaattg ccgggatgat aatcacaccc agcatccagc
    21481 gccatgcacc ggtgtagctg aaggcggtat cagaaagata agcgccgagg atcccgatag
    21541 tgatcatcaa ctgatacatc gagatcatac tgccgcgaat tttttccggc gcaatttctg
    21601 acaggtacag tggtgcggta taagaggcca cacccaccgc cagccccagc agaacgcggg
    21661 aaagaatcag tacttcaacg tttggcgcag ccgcagagaa cagtgaaccg gcaacaaaca
    21721 ggattgcgcc gatcatcagg ctctttttgc gcccgagttt aaaggagagc cagccgctgc
    21781 ccaccgcacc gactgccgca ccgaacatca tggagcttac gacccattct tgcgtgtgcg
    21841 aagtaatctg gaattcatct gcaataaacg gcagtgcgcc agcaattaca ccgatatcca
    21901 ggccaaagag taatcccgcc agagcggcaa ggaagcagac gaaaaacgtc attgccttgt
    21961 ttgaccgccc ctgtttttta gcgtcaggca tgatgccctc caatatggtt attttttatt
    22021 gtgaattaag ataggtgagt acgacgtaaa aagatgtgaa gcaaatcaca tcagtgtaat
    22081 cgcttacact aatatttatc
//
LOCUS       DADSZD010000070        22061 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.070, whole genome shotgun sequence.
ACCESSION   DADSZD010000070 DADSZD010000000
VERSION     DADSZD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.070"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            59..1735
                     /locus_tag="J1Z28_002376"
     CDS             59..1735
                     /locus_tag="J1Z28_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBA7717204.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(1868..3172)
                     /locus_tag="J1Z28_002377"
     CDS             complement(1868..3172)
                     /locus_tag="J1Z28_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBA7717205.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            3324..4292
                     /gene="aes"
                     /locus_tag="J1Z28_002378"
     CDS             3324..4292
                     /gene="aes"
                     /locus_tag="J1Z28_002378"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBA7717206.1"
                     /translation="MQPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDRPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYSGMLHAFLHYSRMMKTADDALRDGARFFSDRLQQD"
     gene            complement(4267..5229)
                     /gene="hemH"
                     /locus_tag="J1Z28_002379"
     CDS             complement(4267..5229)
                     /gene="hemH"
                     /locus_tag="J1Z28_002379"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBA7717207.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVSHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(5361..6005)
                     /gene="adk"
                     /locus_tag="J1Z28_002380"
     CDS             complement(5361..6005)
                     /gene="adk"
                     /locus_tag="J1Z28_002380"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBA7717208.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            complement(6186..8060)
                     /gene="htpG"
                     /locus_tag="J1Z28_002381"
     CDS             complement(6186..8060)
                     /gene="htpG"
                     /locus_tag="J1Z28_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBA7717209.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAERAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(8170..8775)
                     /gene="recR"
                     /locus_tag="J1Z28_002382"
     CDS             complement(8170..8775)
                     /gene="recR"
                     /locus_tag="J1Z28_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBA7717210.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(8775..9104)
                     /locus_tag="J1Z28_002383"
     CDS             complement(8775..9104)
                     /locus_tag="J1Z28_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBA7717211.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(9157..11088)
                     /gene="dnaX"
                     /locus_tag="J1Z28_002384"
     CDS             complement(9157..11088)
                     /gene="dnaX"
                     /locus_tag="J1Z28_002384"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBA7717212.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAATRGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAATELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     gene            complement(11217..11768)
                     /gene="apt"
                     /locus_tag="J1Z28_002385"
     CDS             complement(11217..11768)
                     /gene="apt"
                     /locus_tag="J1Z28_002385"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBA7717213.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(11921..12298)
                     /locus_tag="J1Z28_002386"
     CDS             complement(11921..12298)
                     /locus_tag="J1Z28_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBA7717214.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12368..12895
                     /gene="priC"
                     /locus_tag="J1Z28_002387"
     CDS             12368..12895
                     /gene="priC"
                     /locus_tag="J1Z28_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBA7717215.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREATAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            12909..13070
                     /gene="rsmS"
                     /locus_tag="J1Z28_002388"
     CDS             12909..13070
                     /gene="rsmS"
                     /locus_tag="J1Z28_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBA7717216.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(13282..16644)
                     /gene="mscK"
                     /locus_tag="J1Z28_002389"
     CDS             complement(13282..16644)
                     /gene="mscK"
                     /locus_tag="J1Z28_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBA7717217.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(16772..17419)
                     /gene="acrR"
                     /locus_tag="J1Z28_002390"
     CDS             complement(16772..17419)
                     /gene="acrR"
                     /locus_tag="J1Z28_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBA7717218.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            17561..18754
                     /gene="acrA"
                     /locus_tag="J1Z28_002391"
     CDS             17561..18754
                     /gene="acrA"
                     /locus_tag="J1Z28_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBA7717219.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            18777..21926
                     /locus_tag="J1Z28_002392"
     CDS             18777..21926
                     /locus_tag="J1Z28_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBA7717220.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQIIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         5069 a         5789 c         5855 g         5348 t
ORIGIN      
        1 tgtaattttg gttttcccgg ccaaaaatgg acaataacca ccaggaaaag gagacggaat
       61 gcatcacgcc accccgctta tcaccaccat tgttggcggc cttgtgctcg cctttatcct
      121 cggcatgctg gccaataaac tacgtatttc tcctctggtg ggatatctgt tagcgggtgt
      181 gctggcagga ccattcactc cgggctttgt tgccgatacc aagcttgcgc cggaactggc
      241 tgaactgggc gttattttgc tgatgttcgg cgtcggtttg catttttcgc tcaaggattt
      301 gatggcggta aagtccatcg ccattcccgg cgcgatcgcc cagatagccg tggcgacgct
      361 gctgggtatg gcgctctccg ccgtgctggg ctggtcgtta atgaccggta tcgtgttcgg
      421 cttatgtctt tccaccgcca gtaccgtggt gttactgcgc gcacttgaag aacggcaatt
      481 aattgacagt cagcgtgggc aaatcgccat cggttggttg attgtggaag acctggtgat
      541 ggtcctgacg ctggtattgc tacccgcggt agcaggaatg atggaacagg gcgatgtggg
      601 ctttgccact cttgcagtcg atatggggat caccatcggc aaggtgatcg catttatcgc
      661 cattatgatg ctggtaggtc gccgtctggt gccgtggatt atggcacgca gcgcagcaac
      721 cggttcccgc gagctgttta ccctgtcggt gctggcgctg gcgttaggga ttgcctttgg
      781 tgcggtagag ctgtttgatg tctcctttgc actcggtgcg ttctttgccg ggatggtact
      841 gaacgagtct gaactgagtc accgtgccgc ccacgataca ctgccattgc gcgatgcgtt
      901 cgcggtgctg ttttttgtct ccgtcgggat gttgtttgat ccgttaattc tgattcagca
      961 accgctggca gtactggcga cgctggcaat tattctgttt ggtaaatcgg tcgcggccct
     1021 gttccttgtg cgtttgtttg gtcactccca gcgcacggcg ttaaccatcg ccgccagcct
     1081 ggcgcagatt ggtgagttcg cgtttatcct ggcgggacta ggaatggcat taaatttact
     1141 gccacaggcc ggacaaaacc tggtactggc aggggcgatc ctgtcgatta tgctcaaccc
     1201 ggtactgttc gcactactgg aaaaatatct ggcgaagacc gaaacgctgg aagagcagac
     1261 gctggaagag gcaatcgaag aagagaagca gatcccagtg gatatttgca accatgcgct
     1321 actggtgggt tacggtcgtg taggcagcct gctgggggag aaattgctcg cctctgatat
     1381 tccgctggtg gtgattgaga cgtcacgaac ccgtgttgat gagctgcgcg agcgcggggt
     1441 ccgcgcagta ttgggcaatg cggcgaacga agaaattatg caactggcgc atctggaatg
     1501 tgcaaaatgg ctgatcctga cgattcccaa cggctatgaa gcgggcgaga ttgtggcatc
     1561 tgcccgcgcg aaaaatccgg atattgagat tattgcccgc gcccattatg acgatgaagt
     1621 ggcgtatatc accgaacgtg gtgcgaatca ggtagtgatg ggtgagcgtg aaattgcccg
     1681 tactatgctg gaactgctgg aaacgccacc ggcgggtgag gtggtgacgg ggtaaacggt
     1741 ggttgtcgga tgtaaaatat gaaaaatatg taggcctgat aagacgcgcc agcgtcttat
     1801 caggcatcga gagccaaatg ccagatgcgg cgtgaacgcc ttatctggcc tacgaaccga
     1861 cgataactta acgatcccag taagactctt ccaggctgtc ttcacgctcc ggcaagccgc
     1921 gcgttaaacg aggtgaatgc tggttcagta cctgatagct cacacggtta gcatatttac
     1981 acacctgcgc taacgatgaa taagttaacc aggtgaattt atgtttgctg gagtttggta
     2041 cgttgctacg gtggtagctg ttggcggtaa tgtcatgcag caacgctgcc agtgcgccat
     2101 cccctgctcc attagtgttc atgatttttt ccggcccgcc catgtacggc gcaatgtgcg
     2161 aatagacacg cagcggattc tggcaatcct tgtggcgcat ggcgcggcta aactcatact
     2221 ggttaaattc cgctatagca cccggcagca gcggatgctg ggttttacgt ttcgcttcgt
     2281 cttcggtaaa gcctgccata tacaagccga ttggcccggc ggtgcacagc accagatcta
     2341 cccagtccag cgccttgtca gatgccaaca acggatcgct ttctccggtc aacgcttcgg
     2401 cttcatcttc gttcatcgca aggatagaga cgtggtcttt gaggaattgc tgccaccact
     2461 gcggattctc ggcaatgaca aacttggtgc ccagcgtcag caccaccggt acgttatatt
     2521 tcttcgcgta ctcaatggct ttcatggttg cttccggcat gggttcaccc ggcttgcaac
     2581 gcaccagata agaggtgaga accagtgccg aggctccggc aatcacatct tccggaatac
     2641 tttcagcccg cagctggttc atgtggcccg ggctgatagc aaaggtacgt tccccggact
     2701 cgccaatcag cgtaaagcaa cgaccaatcg ggccatccac gccttgtaga tagttaagat
     2761 cggtacggct ggaggtgtta cacaggtaac gataggcata gctgccaatt tcaatattgc
     2821 tgcacatgac gcccagcagc accgaacggt cgtccgcgag caccgagtag ttgtgcatgg
     2881 tgttaccaat ggtgccaccc gcaaactgat gggtaatcag gtttttctgt tttagttcct
     2941 gataaagcgc ttcggcaacg tcatcctcaa tcaccagtga atgcccggcg cttaatccat
     3001 aacgctcaat gaattcatca tccactttcg cttcaatatc gaccagcgtt tgatcgatac
     3061 ccactaccca ggcggcgctg gtttcgtttt ctggctggaa ttgctgaagc agcggatcgc
     3121 gtgcgtttac ggggaagtaa tgtttggatt tacgtttacc gggaaatttc atggttgttt
     3181 ttacgggtgg ttaactgagc ggcgaatgtt aacacaatct gacgggagcg cgcgagacgc
     3241 agccagtttc aatatcagca ggcgggatcg cattttcatg gcagtggttc cttacaatga
     3301 cgtaatttga aaggagtttt tgcatgcagc cggaaaacaa attacccgtt ctggacctta
     3361 tctctgctga aatgaagacc gttgtgaata ctcttcagcc ggatttaccg ccctggcctg
     3421 caacgggaac gattgctgag caacgacagt attacacgct tgagcgccga ttctggaatg
     3481 tgggcgctcc agaaatggca accagagctt acagggttcc aacaaaatat gggcaggtga
     3541 aaacacgtat cttttatccg cagccagata ggccggcgac gctattttat ttgcatggag
     3601 gcggttttat tctcggcaat ctcgataccc acgatcgcat catgcgcctg ctggcaagct
     3661 acagccaatg tacggtgatt ggcattgatt acaccctttc acctgaagcg cgttttccgc
     3721 aagcgataga ggaaattgtg gctgcctgtt gttatttcca ccagcaggcg gaggattatc
     3781 aaatcaatat gtcccgcatt ggttttgctg gtgattccgc aggtgccatg ctggcgctcg
     3841 ccagtgcgtt gtggttgcgc gataaacaga tcgattgcgg taaagttgcg ggcgttttgc
     3901 tgtggtatgg gctttacgga ttacgggatt ccgtgactcg tcgtctgttg ggtggtgtct
     3961 gggatggctt aacgcaacag gatttgcaga tgtatgaaga ggcgtattta agcaacgacg
     4021 cggaccgcga gtcgccatat tactgcctgt ttaataatga tctcactcgc gaagttccgc
     4081 cctgttttat tgccggggcg gagttcgatc cactgctgga tgacagccgt ctgctttacc
     4141 agacgttagc ggcacaccag caaccttgtg agttcaaact ctactccggt atgctgcacg
     4201 cctttttgca ttattcacgg atgatgaaaa ccgccgacga tgctctacgc gacggtgctc
     4261 gattctttag cgataggctg caacaagatt agccatcatt tcgatatgtt ccggcgtggc
     4321 attaagcgcc ggaatatatt catatttttt cccgcccgca ccgaggaaga cctcacggtt
     4381 ttgctcggca atctcttcca gcgtctccag acaatccgca gcaaagcccg ggcacatcac
     4441 ctgaatatga ctcacgcctt tttctccgag cattttcagc gtttcgtcgg tataaggcat
     4501 cagccagggt tcccgaccaa agcgcgactg aaaggtcatc atcacttttt ccggtgccat
     4561 ctccagtgcg gaagcgagtt cgcgagttgt tgtgcggcaa cgttgcgggt aatcatcgcc
     4621 ttcatctgca taacgctggg gaatgccatg ataagagagc aacagcagat ccggttcgcc
     4681 atgtttggca aaagaagcac gtacgctgtt cgccagtgca ttaatataat cgtggttatc
     4741 ggcgtaatca cgaataaacg atatccccgg aatgctacgt ttgcgcgcca gaatgcgtgc
     4801 cagttcatcc cataccgcac cgaccgttga acaggagtat tgcggataaa gcggcagcac
     4861 cacaatatga tctacatgct ctgccagaag ttcatctacg gcgctttcca gtgatggcga
     4921 gccgtagctc attcccagcg ccacgggcgt ctccggtaaa cgttgcgcca gcgcctgctg
     4981 ttgctggcgg ctgtaaacca tcagcggcga gccaccttcc atccagacag aggcatacag
     5041 cttcgccaca cgcggcgaac gcagcggcaa aatcacgccg cgcagcaacg gccaccataa
     5101 caaccgtgag gtatcaacca cgcgtctgtc gcttaaaaat tgtttcagat agcgttttac
     5161 cgcttcaggt gtgggggcat cgggcgtacc caggtttgcc agcaggatac cggttttagt
     5221 ctgacgcatt accgcctctt atcgattcaa cttgttgata attgtagcgg aaaagtgaag
     5281 aagaagaact aattgctgta agaggtattg cctgaggagg atgagcagac ctgcccgaga
     5341 gcagatctgc ggcggcttga ttagccgaga attttttcca gagctgcgcg aacttcagcc
     5401 accggcttgg tgccgtcaac tttcgcgtat ttggtgttac ccgcttccgc ttctttggag
     5461 tagtagccga tcagcggtgc agtcatctga tggtattcaa ccagacgttt acgcacggtt
     5521 tcttcctgat cgtctttacg ggtagtcagt tcttcaccgg taacgtcgtc tttgccttct
     5581 actttcggcg gattgaattt aacgtgataa acacgaccag acggcgcatg aacgcggcgg
     5641 cctacgatac ggtcaacaat cagttcgtcc ggtacgtcga attccagaac gtaatcaaca
     5701 ttgatgcccg cttctttcat cgcgtctgcc tgcggaatgg tacgcgggaa gccgtccaac
     5761 aggaaaccgt tgcggcagtc ttcctgagca atgcgctctt taaccagcgc gatcaccagt
     5821 tcgtcggtga ccagtttgcc agcatccata atgtcttttg cttgtttacc cagctcggag
     5881 cctgatttga ccgcagcacg cagcatatcg ccagtggaga tttgcggaat accatatttc
     5941 tccatgatga actgagcctg agtccctttc cccgcgcccg gagcgccaag cagaatgata
     6001 cgcattgcga aaatcccctt aaaatgtgtc gaattttttg aaaaagcgat aaacgatacc
     6061 accaacaggc gattgcctca agaaaggcac ctgaggctga aacggttaat ggcgagataa
     6121 tttgcggaaa gtgcagatga agaaaagaaa aatgccggat gacacgaagg tcatccggca
     6181 tggcatcaag aaaccagcag ctggttcata cgacgaataa acaggttcgg atcttccagc
     6241 gtgccgcgtt ctgccagcag cgcctgatcc agcagcagtt ctacccactc gctgaacttc
     6301 gcttcatctt cagtatctgc cgcacgtttc accagtacgt gatccgggtt cagttcgaag
     6361 atgtatttca cttccggcac tttctggccc gctgcggcaa acagtttcgc catctgggtg
     6421 ctcatttcgt ccgcgtcggt agagacaatc gctggcgtat cggtcagacg gtgagtcaga
     6481 cggacatctt tcacgcgctc gccgagcagg gctttcacac ggtcgatgaa cggagtcagt
     6541 gctctctccg cttctttcgc gctctcatca acttcgtcag ccagtttttc cagcgactcg
     6601 tcaactttag acaccgactg gaacggttta ccgtcgaact cagtcagata gttcatcatc
     6661 cactcatcga tgcggtcgga aagcagcaga acttcgatgc ctttcttacg cagcagttcc
     6721 aggtgcgggc tgctcttcgc tgccgcatag ctgtctgcgg tgatgtagta gattttctcc
     6781 tgcccttctt tcatgcggga aacgtagtct tccagagaca cggtctgcgc agaagaatcg
     6841 gtatgggtag aagcaaaacg cagcaatttg gcgatcgctt cctggttagc gaaatcttcc
     6901 gccggacctt ctttcagtac caggccaaac tgttgccaga aggtctggta tttttccgcg
     6961 tcgtctttcg ccagtttttc cagcatttgc agcacacgct tggtcagcgc attgcgcagg
     7021 ttacgcgtta ccgtgctgtc ctggaggatt tcacgggaaa cgttcagcgg cagatcgctg
     7081 gagtcaatca gaccacgcac gaagcgcaga tagttcggca tgaactgttc tgcgtcgtcc
     7141 atgatgaaca cacgctgaac atacagtttc agaccgtgtt tatgatcgcg gttccacata
     7201 tcccacggtg cctgagacgg aatgtacagc aggctggtgt actcctgctt accttcaacg
     7261 cggttgtggc tccaggtcag cggatcgtta aagtcgtggg cgatgtgttt gtagaactct
     7321 ttgtactctt catcggtgat ttccgacttg ttacgagtcc acagcgcctg cgctttgttg
     7381 attttctccc aggagataac ggtttcgccg tctttctctt cgcgtttttc gatctctacc
     7441 ggcagcgcga tatggtcgga atatttgctg atgatggaac gcacacgcca gtcatcgagg
     7501 aactcgtctt cgccttcacg cagatgcagg gtgatttcag taccacgatc ttctttagtg
     7561 atgtcggcaa cggtgtattc accttcgcca gccgattccc agaagacgcc gttttctggt
     7621 ttttcgcctg ccgcgcgagt acgcacggtc actttgtcag ccacgataaa cgcagagtag
     7681 aaaccaacac caaactgacc gatcagttgg ctgtctttcg cctggtcaga gcccagggat
     7741 tcgaggaatg atttggtgcc ggatttagcg atagtcccca gatggtcaat cacttcgtcg
     7801 cgggtcatcc ccacgccgtt atcggagatg gtcagcgtgc gcttgtcttt atcgaaagag
     7861 acacgaacgc gtagttcacc atcaccttcg tacaggtccg ggttagaaag cgcgcggaaa
     7921 cgcagcttgt ccgccgcatc ggaggcgtta gagataagct cacgcaggaa gatttcttta
     7981 ttggaataga gagaatggat catcaggtgc agaagctgct tcacttctga ctgaaaacca
     8041 cgagtttctt gtcctttcat gtaggtctac ctcaataatg ccattttaag gttaaaaaca
     8101 ggatgtggga gagatgggga caagggagaa taatttcaag cgagagcagg tgatcctgct
     8161 ctcgtttgct taaaaacgaa tcttatgacg cccggcaagg gagtgtgaca gcgtggtgcc
     8221 atcgaccatt tccagctcgc cgccaaccgg tacgccatga gcgattcggc tggcttccac
     8281 gtcatattgc gcgcaaagct cggcaatgta gttagcggta gcttcacctt caaccgtggg
     8341 gttggtggcg aggatcactt cagtgatttt ttcctctgcc agacgctgtt ccagacgatc
     8401 aaggccgata tcatccggac cgatgccgtc cagcggtgac agatgcccca tcaacacaaa
     8461 ataacgacct gaaaactgcc ccgtctgctc aatggcgtaa atgtccgccg gactctccac
     8521 cacgcagatt tgaccgtttt cctgacgacg cggattcgaa cagatgttac agacttcctg
     8581 ttcggtgaaa gtgcggcaat cggcgcagtg gccgatttcc gacatcgccc gggtgagcgc
     8641 ctgcgccaga cgcatcccgc cgctacgatc gcgctgaagc agcgtgaacg ccatacgctg
     8701 cgccgacttc gggccaacgc ccggcagaca gcgcagtgct tccataagct gtgttaacag
     8761 cgggctggtt tgcatcagaa cggcatctta aagccaggcg gcagctgcat tccggaggat
     8821 acagaggcca ttttttcttt ctgcgtttct tcaatacgac gtgctgcgtc gttgaatgct
     8881 gcagccacca ggtcttccag catctctttg tcgtcttcca gcaggctcgg gtcgatctct
     8941 acgcgacggc agttgtgtgc accgttgatg gtcactttta ccagacctgc gccagattcg
     9001 ccggtgactt ccagctgcgc gatctcttcc tgcattttct gcattttttc ttgcatctgc
     9061 tgggcttgct tcatcaggtt acccagaccg cctttaccaa acataggttt ctctctcaat
     9121 cacgttaagg atgacgaacg taagctgtgc ttacgatcaa atggggcgga tactttcttc
     9181 atccagctcc gcatcgaaga atcgacgcag ggtctgaata ttattatccg caataatgga
     9241 ctcgcgcgcc tgcgcaagtt tttcttcgta tatcgcctga cgccattcca gcggcgtacg
     9301 caccgcggga ttatcatctt caacgatagt cagttcaacc gttgaacctt ttaacgtgct
     9361 caacgcttca gccagttttt gctgtgcgcc gcggttgttc aaatgccgct gagaggagcg
     9421 caaatgcaga catactgcgt tgtcgctctc ctctttccag gcatttaacg ccacctgttc
     9481 gaccagtttt ggtagcgaaa gttgactcac ctgagccgcc cacgggtcgc gctcaatggc
     9541 ttctgccgcc agcttcgccg ccagttccgg cgttttttca tgttccagcg cttttttcag
     9601 cgccttcggc gtggcgacca cttctttttg ctgcatcact ggtgtggtcg ccttccagcg
     9661 atacgcttct tttttggctg gcgctttttc cagcgccgat ggcaccggac gcgcctgaac
     9721 gcgatcggtg accgaagcca gtctttccag cgcagcgtta ttcaccggcc gcgcgcgggt
     9781 agcggctgcc ggttcactct tttttgcttt ggttgctccc tgcacgcgct gcaactgctg
     9841 gcgcgccgcc agcacctggc tggtggtttc cgggagcggt acagtcggtg cctgctgcgg
     9901 cgctgattgc ggttgcggcg gcacctgggt tggcgtcatt actgccgttg gcgcgacggg
     9961 cgcaaaggac tggcgtggca cttctggctc aggcagcggc atacgcggat ggaacgccag
    10021 cgcgcgcagc agcgtcatct caacacccat acggcggtct ggcgcatacg gtaattcttt
    10081 gcgaccaatc aacagcgtct gatagtaaag ctgaatatcc gtcggcggta tggtgcgcgc
    10141 cagttcacgc atccgcagct cagtggcggc catgtcgttg ccaagagcag caggcgaaag
    10201 ttgtaccatc gcaatacgat gcaacagacc gagcatttcc accagcaacg cttcccactc
    10261 gataccacgg gtagcagctt cattaattag cgccattacg cgctcgccgt tggcctcgac
    10321 catcgcttca accagcgata acgcctgatc gtcgtcaagc gtacccagca tcgcactgac
    10381 cgcctgggtt gaaacctggc cgtcaccgct ggcaatcgcc tggtcggtca gacttaaggc
    10441 atctcgcagg ctgccttcag cggcgcgtgc caacaattgc agcgcccgcg gctcgtgagc
    10501 gatatgttct tcgttgagga tgtgctcaag ctgatggcga atttgctcga catccagcgc
    10561 cttgagatga aattgcagac agcgtgacaa aatcgtcacc ggcaatttct gcggatcggt
    10621 cgtcgccagc aaaaacttaa cgtgctctgg cggctcttca agggttttta acagtgcgtt
    10681 aaagctgtga cgcgacagca tatgcacttc gtcgatgaga taaactttga aacgaccacg
    10741 cgccggagcg tactgaacgt tatccagcag gtcgcgggta tcttcgactt tagtgcgcga
    10801 ggcggcgtcg atttcaatca gatcgacaaa gcgcccctgc tcgatttcac gacagttatc
    10861 gcacacgccg cacggcgtcg cggtaatgcc ggtttcgcag tttagcccct tcgccaacag
    10921 tcgggcgata gaggtttttc cgacgccacg ggtgccggaa aaaagataag catgatgaat
    10981 acgccctaac gacaagccgt tcgccagtgc ggtcagcaca tgttcctggc cgacgacgtc
    11041 agcaaaggtt tgtgggcgcc attttcgggc taagacctga taactcattg gcaggctctg
    11101 aaacgctgga aggtggattc acgaaggggt aatgctaaca cagccccgtc agaacggcga
    11161 ggctgaatgc acatcttgtg ctgcccgtag cgtgagcagc gcaagactga cgataattaa
    11221 tggcccggga acgggacaag gctgtagctg gtaatgccct gtttttcgag acgctgttca
    11281 ccgccgagat cgaacaggtt gataatgaac gcagcgtcag ctacttcacc acccagacga
    11341 cggatcagtt taacggtcgc ttcgatagtg ccgccggttg ccagcagatc gtccaccacc
    11401 agaactttat cgcccggttt gatggcatca acgtggattt ccagctgatc ggtgccgtat
    11461 tccaggtcgt aagtttcact gatggtttca cgcggcagtt tgcccggttt acggaccggt
    11521 acaaagccaa cgcccagacc cagagctacc ggagcgccaa acaagaagcc acgcgcttcg
    11581 gtgccgacca ctttggtaat gcccgcattt ttgtaacgct caaccagcaa gtcgatgctg
    11641 agagcgtaag ctttcgggtc ttccagtaag ctggtgacat cgcggaaaag aatgccgggt
    11701 tttgggtagt cctgaatgct tttgatgcta tttttgagat actcaagctg ctgtgcagtc
    11761 gcggtcataa gtgtatgcct gcttgttacg gtggtactca cggcgcgttt tgaacgtatc
    11821 aaaagtaaca gttgtttaac ctttgaccgc ctgcgcctgt gctcgaaaac gccagaatgt
    11881 actggctgta cgcaataatt gcaactgcga ttgttgtgct tcagtgcttt tcttgctttt
    11941 catcaatcac cggaattcgc cacatataaa aaagcaaaca ggcgagaatt accagcaaca
    12001 tgatgcgcac ccacggcatc tggacgaacc acagcgaaat cgtaaacgta atcaaaataa
    12061 gcaaaatcgc ccgtggtttt gcgccgcgcg gcatcgcatg atgtttctgc cagaaacgta
    12121 gatagctgcc aaaccatgag cggtacagca accaggcgtg aaagcgcggg gaagaacggg
    12181 caaagcacca ggccgccagc aggataaacg gcgtcgtcgg taataccggt aataccacgc
    12241 ccagcgtacc cagcactacc gccagccagc caatgatgat taaaatgatt cgttgcatat
    12301 tgagtgttgt cagcttacag agtggctact ttagcataac aattatcatt ttcattgagg
    12361 tcttatcgtg aaaaccgccc tgctactgga aaaactggaa ggacagctcg ccacgctgcg
    12421 tcagcgttgt gccccggtag cgcaatttgc cacgctaagt gcccgttttg acaggcacct
    12481 ttttcagacc cgtgcgacaa cactacaggc ttgtctcgat gaggcgggcg ataacctggc
    12541 tgcgcttcgt catgcggttg agcagcaaca gctgccgcaa gtcgcctggc tggcagaaca
    12601 tctggcggcg caactggagg ctatagcgcg tgaagccaca gcctggtcgc tacgcgagtg
    12661 ggatagcgca ccaccgaaaa ttgcccgctg gcagcgtaaa cgtattcagc atcaggattt
    12721 tgagcggcgg ctacgtgaga tggttgccga acgtagagcc cgtctggcac gagcgaccga
    12781 tctcgtggaa cagcagacgc tgcatcgtga agtggaagcc tatgaagcgc gcctggcacg
    12841 ctgccgccat gcgctggaaa aaatcgaaaa caggttagcg cgtttaaccc gctagcaatg
    12901 gagagagtat gtcactggaa aatgcccctg acgatgtcaa actggccgtc gatttgattg
    12961 tgctgctgga agaaaatcag atcccagccc gcaccgtgct gcgcgcgctg gatattgtaa
    13021 aacgcgatta tgaaaagaaa ttaacgcgcg atgatgaggc ggaaaagtga gaaataaatg
    13081 gggccgctaa atgtccccta agattgtggg caaacgcaaa actgcctgat gcgctacgct
    13141 tatcaggcct acaccgctcc tgcaatatat tgaattcttg cggtttcgta ggccggataa
    13201 ggcggtcacg ccgcatccgg catgaacaac gcgcactttg ttagtaatct cggggcaact
    13261 aaatgtcgcc ccctttggtt attacccaac cgctggtgtc ggatcgtcgc ctttgtagtc
    13321 gcgtttgact tccgtcacct catcgccctt ctcgttgtgc agatgcactt caagctggtt
    13381 aaaggcaatg ttgatgtcgt tttcacggca caactgatcg atagtacggt tcagctcatc
    13441 gacagtacgg ctacggtcac gcagttcacg cacatacaga cgcagctcat gatccaacgt
    13501 gctggcacca aatgccgtaa agaagacttc cggcattggt tcgtgcatca cccttgggtg
    13561 ctcagtcgcc gccttcagca acactttacg cactttttcc agatcggagc cataagctac
    13621 gccgagacgg atcaccagac gcgtagtcgt gtcagtcaac gaccagttga tcagacgctc
    13681 ggtaacaaac gctttgttcg ggatgatcac ttctttgcga tcgaaatcgg taatcgttgt
    13741 cgcacgaata cggatcttac ttaccgtccc cgagaagcta ccaatggtta ccgtgtcgcc
    13801 tatgcgcacc ggacgttcga acagaatgat caaaccggaa acgaagttac cgaagatctc
    13861 ttgtaaacca aaaccaagac ctacggataa tgctgccgcc agccactgga gtttatccca
    13921 cgagacgccc agcgatccga acaccgtcat cgcaccaacg gcaataatga tgtagttaag
    13981 gatggtagta atggcatacg acgcgccctg gcgcatattc agtcgcgaga gcaccagcac
    14041 ttccagcaaa ccaggcaggt tgcgaatcaa cgcccaggcc accattgagg cgataatcgc
    14101 aaacaacaga ctgcccatgg tgacgttttt caccaccgca gcaccagctt cagtagcgtt
    14161 gtaatgccag agcgtaatgc tgtcgagata gctgaacacg gtgatcaaat cagaccaaat
    14221 tgcccagaac atgacaccga acagcgcaaa catcagcaac atggtaatac gcagcgtctg
    14281 ctggttaacc tgctccagcg caatggtggg ttcttccggc ggttcggcac cttctgcgcc
    14341 ctctttcacc agattctgcc gacgcgccag tgcacgacgc caggcgatac gccgcgccgc
    14401 tacgcttaag ccgcgcaata ccgtctggta cagcaggttc cagatgatca ccaaataaac
    14461 ggtttcaatc caacgcccgg agaggcgcag cgtggtgtag aagtaacccg tggcggtcag
    14521 caccatcaac gcaatcggga taatcgacag cacggtaatg gtgaccagtc gcatggtgtg
    14581 cgactcttta tcacgccagc tttcgcggca catcggccac accaggaagg caatcagcag
    14641 caggttgaag aaaatcatcg cctgccccag cacatcatcc atcagatgca gcggggagag
    14701 ttctgccacc acagaccaga aatggatcgg cagcaacgcg aggctgatgc ggacaatttg
    14761 ccgacgccag tggctggtct gctgttccgg cataccaaag tgacgcacgg caacaccgtt
    14821 tttctccagc actttccagc acaggccaaa caccagccag aaaatcgcca gttttttgct
    14881 gaacgaccac aataactcgc tgatattgag ctgcatagtc agcagaatca ggccgacagc
    14941 gagaataatc aggcacaccg gcagtgcgcg gattaggtca ataagaatcg cttttggcgt
    15001 attgagctgg ctatcgttac gcagggaacc taccgccgaa gccagttttt gttgatatgc
    15061 tttcagccag cctaaacgcc agtgaatcag cccggcaatc aacagcagcg gcaaaccagc
    15121 gaggaaggcg atgaatacgg cgggccaggc tttttcccag ttcaccgtga ttttcatcga
    15181 cttaaattca tctttcagcg tttgcgggaa cgctttaatc cagtcccagt ccatcgggcg
    15241 gttactgttc acccaaaaaa tttgctgcgt caggatggat ttcaggtttt tcgacacact
    15301 cattaactgc tgctggttga tttgcaggtt aatggccatc atcagctggt tacccaactg
    15361 tttgttaagc tgatccagca attcgcgacg catatcaact acttgcaata atgcatcgtg
    15421 aacttcgctg ttaacttcat tggtgtgacc ttcttccagt ttgctgacga acgcatcgct
    15481 ctggaacagc gcgtcacgct gctggttgac ttcaaactgt tcgagacgca aatccgcgat
    15541 gcggttggtc atgttttcca gttcatccgc cgagggcagc gtttgttgtt gctggtaaag
    15601 aatacgagac aacagcaggc tgcccttcag gacggcaatc tgctctttaa tattgcgttc
    15661 cgattgcagc gcacgctcca gccagttttt aactttgatg ttttgctgca tcaactgatt
    15721 accgttttca gtcgcggtaa tcagacgctg acttaactgc tggttaattt ccagttcctg
    15781 cttcaccagc ggattagcct gaatacgcgc ggcttcatcc ggggagacag cttcctgcgc
    15841 cgttttctcg gttaaagtca ggcgcttgct gtttaccgct tcttgcaaca gttgtaactg
    15901 gtgctccaga cgggcgctgt tcgccgtcac gtaatcacgc tgcttttgca aggtatcctg
    15961 caagacagtg ttcccttcca ggcttttacg ctgctggtca atctcggcat tcagcaaagc
    16021 ctgctgggcc tgcattaaca ctttctggct gggacgtaag gccgtctcgc cgacatcagt
    16081 cccatccaga cgactgcgaa tttgttgcag ctgctgcgaa gcgttataca tcgcattttg
    16141 cacgcgttcg ggctgcgtct gtaacgaaac cagttggctg ttataagacg ccagatcgtt
    16201 ttgtgcgttt tgcaaatcgt ccagcgcctg ggcaacgcga gtttccagct ggcgcaacga
    16261 aagcgtgctc agaattttgc gcgtttcttc gtcgttatcg acatcactaa gtgctgttaa
    16321 cgccgctgtc gcctggcgca ttttttccgg cgcttcagcg actttttgcc gtagctgaac
    16381 tgtctcttct tttacgcgat cgattttatc gagggtggct aatgtatctg tcagatcctg
    16441 ctgcaccagt ttgtcctgag cagaaagatc tttttgttta ttcagtgagt caagttgtgc
    16501 ctgcaggtcc gcttttgtcg gtaaatcacc attcgatgac gcccgtgcaa acgccgtgtt
    16561 ctggcataac aagacaaaaa caaaagcaat aaatgctgaa aaaacaaaat gccgtgatcg
    16621 tttgtaatac tggaacatag tcatgatgaa tgaaggtttc tgaacctgaa gaacgacctg
    16681 aaaaagtcaa accgcaagaa tatcacgacg cagtgaacca gaatagcaac gacgaaaatg
    16741 tccaggaaaa ttcctggagt cagattcagg gttattcgtt agtggcaggg ttacgaagcg
    16801 tggggcacag gagatacatc tccagtaaga tggcgacgta atcgcgggct tcttttttaa
    16861 gatcaaaaga ttgcggggca aagagccagt tttccatcag gccggaaata tagccgcgca
    16921 taataattgc tgcgcgacgc gtcattaaat ccgcaggcaa cattttcgct tcaatacaat
    16981 gttttaacgt ttgttctata cggtcataac tttccagaca gagattacgt tgtgcctgtt
    17041 gcacaacagc catttcgccg acaaattcgc atttgtggaa tataatctcc atcaataatc
    17101 gacgccgttc ttctgtcacc gtggactcaa gaacatgaat taatatctct cttaatactg
    17161 agagtggatc gccagggaat tttgcctgat actcaagctc gagttcacca atattggatt
    17221 ctgacagttc ccagatctca ctgaacaaat ccgacttgtc tttaaaatgc cagtagattg
    17281 caccgcgcgt aacgccagct gcttttgcaa tctcacccag cgaggtggat gataccccct
    17341 gctgtgagaa aagacgtaga gccacatcga ggatgtgttg gcgcgtttct tgcgcttctt
    17401 gtttggtttt tcgtgccata tgttcgtgaa tttacaggcg ttagatttac atacatttgt
    17461 gaatgtatgt accatagcac gacgataata taaacgcagc aatgggttta ttaacttttg
    17521 accattgacc aatttgaaat cggacactcg aggtttacat atgaacaaaa acagagggtt
    17581 tacgcctctg gcggtcgttc tgatgctctc aggcagctta gccctaacag gatgtgacga
    17641 caaacaggcc caacaaggtg gccagcagat gcccgccgtt ggcgtagtaa cagtcaaaac
    17701 tgaacctctg cagatcacaa ccgagcttcc gggtcgcacc agtgcctacc ggatcgcaga
    17761 agttcgtcct caagttagcg ggattatcct gaagcgtaat ttcaaagaag gtagcgacat
    17821 cgaagcaggt gtctctctct atcagattga tcctgcgacc tatcaggcgg catacgacag
    17881 tgcgaaaggt gatctggcga aagcccaggc tgcagccaat atcgcgcaat tgacggtgaa
    17941 tcgttatcag aaattgctcg gtactcagta catcagtaag caagagtacg atcaggctct
    18001 ggctgatgcg caacaggcga atgctgcggt aactgcggcg aaagctgccg ttgaaactgc
    18061 gcgaatcaat ctggcttaca ccaaagttac ctctccgatt agtggtcgca ttggtaagtc
    18121 aaacgtgacg gaaggcgcat tggtacagaa cggtcaggcg actgcgctgg caaccgtgca
    18181 gcaacttgat ccgatctacg ttgatgtgac ccagtccagc aacgacttcc tgcgcctgaa
    18241 acaggaactg gcgaatggca cgctgaaaca agagaacggc aaagccaaag tgtcgctgat
    18301 caccagtgac ggcattaagt tcccgcagga cggtacgctg gaattctctg acgttaccgt
    18361 tgatcagacc actgggtcta tcaccctacg cgctatcttc ccgaacccgg atcacactct
    18421 gctgccgggt atgttcgtgc gtgcacgtct ggaagaaggg cttaatccaa acgctatttt
    18481 agtcccgcaa cagggcgtaa cccgtacgcc gcgtggcgat gccaccgtac tggtggttgg
    18541 cgcggatgac aaagtggaaa cccgtccgat cgttgcaagc caggctatcg gcgataagtg
    18601 gctggtgaca gaaggtctga aagcaggcga tcgcgtagta ataagtgggc tgcagaaagt
    18661 gcgtcctggt gtccaggtaa aagcacaaga agttaccgct gataataacc agcaagccgc
    18721 aagcggtgct cagcctgaac agtccaagtc ttaacttaaa caggagccgt taagacatgc
    18781 ctaatttctt tatcgatcgc ccgatttttg cgtgggtgat cgccattatc atcatgttgg
    18841 caggggggct ggcgatcctc aaactgccgg tggcgcaata tcctacgatt gcaccgccgg
    18901 cagtaacgat ctccgcctcc taccctggcg ctgatgcgaa aacagtgcag gacacggtga
    18961 cacagattat cgaacagaat atgaacggta tcgataacct gatgtacatg tcctctaaca
    19021 gtgactccac gggtaccgtg cagatcaccc tgacctttga gtctggtact gatgcggata
    19081 tcgcgcaggt tcaggtacag aacaaactgc agttggcgat gccgttgctg ccacaagaag
    19141 tacagcagca aggggtgagc gttgagaaat catccagcag cttcctgatg gttgtcggcg
    19201 ttatcaacac cgatggcacc atgacgcagg aggatatctc cgactacgtg gcggcgaata
    19261 tgaaagatgc catcagccgt acgtcgggcg tgggtgacgt tcagttgttc ggttcacagt
    19321 acgcgatgcg tatctggatg aacccgaatg aactgaacaa attccagcta acgccggttg
    19381 atgtcattac cgccatcaaa gcacagaacg cccaggttgc agctggtcag ctcggtggta
    19441 cgccgccggt gaaaggccaa cagcttaacg cctctattat tgctcagacg cgtctgacct
    19501 ctactgaaga gttcggcaaa atcctgctga aagtgaatca ggatggttcc cgcgtgctgc
    19561 tgcgtgatgt ggcgaaaatt gagctgggtg gtgagaacta cgacatcatc gcagagttta
    19621 acggccaacc ggcttccggt ctggggatca agctggcgac cggtgcgaac gcgctggata
    19681 ccgctgcggc aatccgtgct gaactggcga agatggaacc gttcttcccg tcgggtctga
    19741 aaattgttta cccgtatgac accacaccgt tcgtgaaaat ctctattcac gaagtggtta
    19801 aaacgctggt cgaagcgatc atcctcgtgt tcctggtaat gtatctgttc ttgcagaact
    19861 tccgcgcgac gttgattccg accattgccg taccggtggt attgctcggg acctttgccg
    19921 tccttgccgc ctttggcttc tcgataaaca cgctaacaat gttcgggatg gtgctcgcca
    19981 tcggcctgtt ggtggatgac gccatcgttg tggtagaaaa cgttgagcgt gtgatggcgg
    20041 aagaaggttt gccgccaaaa gaagccaccc gtaagtcgat ggggcagatt cagggcgctc
    20101 tggtcggtat cgcgatggta ctgtcggcgg tattcgtacc gatggccttc tttggcggtt
    20161 ctactggtgc tatctatcgt cagttctcta ttaccattgt ttcagcaatg gcgctgtcgg
    20221 tactggtggc gttgatcctg actccggctc tttgtgccac catgctgaaa ccgattgcca
    20281 aaggcgatca cggggaaggt aaaaaaggct tcttcggctg gtttaaccgc atgttcgaga
    20341 agagcacgca ccactacacc gacagcgtag gcggtattct gcgcagtacg gggcgctacc
    20401 tggtgctgta tctgatcatc gtggtcggca tggcctatct gttcgtgcgt ctgccaagct
    20461 ccttcttgcc agatgaggac cagggcgtat ttatgaccat ggttcagctg ccagcaggtg
    20521 caacgcagga acgtacgcag aaagtgctca atgaggtaac gaattactat ctgaccaaag
    20581 aaaagaacaa cgttgagtcg gtgttcgccg ttaacggctt cggctttgcg ggacgtggtc
    20641 agaataccgg tattgcgttc gtttccttga aggactgggc cgatcgtccg ggcgaagaaa
    20701 acaaagttga agcgattacc atgcgtgcaa cacgtgcttt ctcgcaaatc aaagatgcga
    20761 tggttttcgc ctttaacctg cccgcaatcg tggaactggg taccgcaacc ggctttgact
    20821 ttgagctgat tgaccaagct ggccttggtc acgaaaaact gactcaggcg cgtaaccagt
    20881 tgcttgcaga agcagcgaag caccctgata tgttgaccag cgtacgtcca aacggtctgg
    20941 aagatacccc gcagtttaag attgatatcg accaggaaaa agcgcaggcg ctgggtgttt
    21001 ctatcaacga cattaacacc actctgggcg ctgcatgggg cggtagctat gtgaacgact
    21061 ttatcgaccg cggtcgtgtg aagaaagttt acgtcatgtc agaagcgaaa taccgtatgc
    21121 tgccggatga tatcggcgac tggtatgttc gtgctgctga tggtcagatg gtgccgttct
    21181 cggcgttctc ctcttctcgt tgggagtacg gttcgccgcg tctggaacgt tacaacggcc
    21241 tgccatctat ggaaatctta ggccaggcgg caccgggtaa aagtaccggt gaagcaatgg
    21301 agctgatgga acaactggcg agcaaactgc ctaccggtgt tggctatgac tggacgggaa
    21361 tgtcctatca ggaacgtctc tccggcaacc aggcaccttc actgtacgcg atttcgttga
    21421 ttgtcgtgtt cctgtgtctg gctgcgctgt acgagagctg gtcgattccg ttctccgtta
    21481 tgctggtcgt tccgctgggg gttatcggtg cgttgctggc tgccaccttc cgtggcctga
    21541 ccaatgacgt ttacttccag gtaggcctgc tcacaaccat tggtttgtcg gcgaagaacg
    21601 cgatacttat cgtcgaattc gccaaagact tgatggataa agaaggtaaa ggtctgattg
    21661 aagcgacgct tgatgcggtg cggatgcgtt tacgcccaat cctgatgacc tcgttggcgt
    21721 ttatcctcgg cgttatgccg ctggttatca gtactggtgc tggttccggc gcgcagaacg
    21781 cagtaggtac cggtgtaatg ggcgggatgg tgaccgcaac ggtactggca atcttcttcg
    21841 ttccggtatt ctttgtggtg gttcgccgcc gctttagccg caagaatgaa gatatcgagc
    21901 acagccatac tgtcgatcat cattgataca acgtgtaatc actaaggccg cgcaagcggc
    21961 cttttttatg cataacctac gaacattaag gagtattgaa ccaccaactc aggatctcat
    22021 acgaaaacca gtattaacca cgtataaaat tcataaaaaa t
//
LOCUS       DADSZD010000071        21710 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.071, whole genome shotgun sequence.
ACCESSION   DADSZD010000071 DADSZD010000000
VERSION     DADSZD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.071"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(135..467)
                     /gene="emrE"
                     /locus_tag="J1Z28_002393"
     CDS             complement(135..467)
                     /gene="emrE"
                     /locus_tag="J1Z28_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBA7717221.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(812..1126)
                     /gene="fliE"
                     /locus_tag="J1Z28_002394"
     CDS             complement(812..1126)
                     /gene="fliE"
                     /locus_tag="J1Z28_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBA7717222.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            1341..2999
                     /gene="fliF"
                     /locus_tag="J1Z28_002395"
     CDS             1341..2999
                     /gene="fliF"
                     /locus_tag="J1Z28_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body M-ring protein FliF"
                     /protein_id="HBA7717223.1"
                     /translation="MNATAVQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESQAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSEESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2992..3987
                     /gene="fliG"
                     /locus_tag="J1Z28_002396"
     CDS             2992..3987
                     /gene="fliG"
                     /locus_tag="J1Z28_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBA7717224.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3980..4666
                     /gene="fliH"
                     /locus_tag="J1Z28_002397"
     CDS             3980..4666
                     /gene="fliH"
                     /locus_tag="J1Z28_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBA7717225.1"
                     /translation="MSDNLPWKTWMPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            4666..6039
                     /gene="fliI"
                     /locus_tag="J1Z28_002398"
     CDS             4666..6039
                     /gene="fliI"
                     /locus_tag="J1Z28_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBA7717226.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6058..6501
                     /gene="fliJ"
                     /locus_tag="J1Z28_002399"
     CDS             6058..6501
                     /gene="fliJ"
                     /locus_tag="J1Z28_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBA7717227.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            6498..7625
                     /gene="fliK"
                     /locus_tag="J1Z28_002400"
     CDS             6498..7625
                     /gene="fliK"
                     /locus_tag="J1Z28_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBA7717228.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDIVSDAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQADDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            7730..8194
                     /gene="fliL"
                     /locus_tag="J1Z28_002401"
     CDS             7730..8194
                     /gene="fliL"
                     /locus_tag="J1Z28_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBA7717229.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8199..9203
                     /gene="fliM"
                     /locus_tag="J1Z28_002402"
     CDS             8199..9203
                     /gene="fliM"
                     /locus_tag="J1Z28_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBA7717230.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9200..9613
                     /gene="fliN"
                     /locus_tag="J1Z28_002403"
     CDS             9200..9613
                     /gene="fliN"
                     /locus_tag="J1Z28_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBA7717231.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSGKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            9616..9981
                     /gene="fliO"
                     /locus_tag="J1Z28_002404"
     CDS             9616..9981
                     /gene="fliO"
                     /locus_tag="J1Z28_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBA7717232.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            9981..10718
                     /gene="fliP"
                     /locus_tag="J1Z28_002405"
     CDS             9981..10718
                     /gene="fliP"
                     /locus_tag="J1Z28_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBA7717233.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            10728..10997
                     /gene="fliQ"
                     /locus_tag="J1Z28_002406"
     CDS             10728..10997
                     /gene="fliQ"
                     /locus_tag="J1Z28_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBA7717234.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11006..11791
                     /gene="fliR"
                     /locus_tag="J1Z28_002407"
     CDS             11006..11791
                     /gene="fliR"
                     /locus_tag="J1Z28_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBA7717235.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLALIKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            12081..12704
                     /gene="rcsA"
                     /locus_tag="J1Z28_002408"
     CDS             12081..12704
                     /gene="rcsA"
                     /locus_tag="J1Z28_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBA7717236.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(12748..12990)
                     /gene="dsrB"
                     /locus_tag="J1Z28_002409"
     CDS             complement(12748..12990)
                     /gene="dsrB"
                     /locus_tag="J1Z28_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBA7717237.1"
                     /translation="MPEKRNCSTLAQSHRRKTMKVNDRVTVKTDGGPRRPGVVLAVEE
                     FSEGTMYLVSLEDYPLGIWFFNEAGHQDGIFVEKAE"
     gene            13099..13326
                     /gene="yodD"
                     /locus_tag="J1Z28_002410"
     CDS             13099..13326
                     /gene="yodD"
                     /locus_tag="J1Z28_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBA7717238.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            13624..14439
                     /locus_tag="J1Z28_002411"
     CDS             13624..14439
                     /locus_tag="J1Z28_002411"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBA7717239.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSCLHEANIPVI
                     LCSSKTSAEMLYLQKMLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEICQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FIARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGRRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFPAR"
     gene            complement(14436..16130)
                     /gene="yedQ"
                     /locus_tag="J1Z28_002412"
     CDS             complement(14436..16130)
                     /gene="yedQ"
                     /locus_tag="J1Z28_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBA7717240.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAVYVSRAGFYVSTLPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTSMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKACPLAKLCHTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDIAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(16301..16483)
                     /locus_tag="J1Z28_002413"
     CDS             complement(16301..16483)
                     /locus_tag="J1Z28_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBA7717241.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(16562..17479)
                     /locus_tag="J1Z28_002414"
     CDS             complement(16562..17479)
                     /locus_tag="J1Z28_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBA7717242.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            17652..18572
                     /gene="yedA"
                     /locus_tag="J1Z28_002415"
     CDS             17652..18572
                     /gene="yedA"
                     /locus_tag="J1Z28_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBA7717243.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(18561..19031)
                     /locus_tag="J1Z28_002416"
     CDS             complement(18561..19031)
                     /locus_tag="J1Z28_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HBA7717244.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGRKKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19012..20430)
                     /locus_tag="J1Z28_002417"
     CDS             complement(19012..20430)
                     /locus_tag="J1Z28_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HBA7717245.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(20497..21192)
                     /locus_tag="J1Z28_002418"
     CDS             complement(20497..21192)
                     /locus_tag="J1Z28_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBA7717246.1"
                     /translation="MELQHWQAQFENWLKNHHQHQDAAHDLCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGLALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLGAELAGENEG
                     IDHKVIDAFSPAG"
     gene            complement(21232..21597)
                     /locus_tag="J1Z28_002419"
     CDS             complement(21232..21597)
                     /locus_tag="J1Z28_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA7717247.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGGIAWYTRPRPARCRRYE"
BASE COUNT         5298 a         5728 c         5574 g         5110 t
ORIGIN      
        1 tttgatgtgc cttgatctca ttatgcataa aggcgcaatg atcgcgcaaa tgcaatcata
       61 attataaagg tgccccgaac attagccata atatgcacaa atcagattcc tgttatctca
      121 aggataatta ttttttaatg cggtgcactt cgtgacaaca agttaatgac taacacaccg
      181 gcacaaatca acatcatgcc tatgatagct ggcaggtcca gtcgttgacc aaaaaatccc
      241 caggaaagca aactaatcag aacgataccg actcctgacc agatagcata agcaatccct
      301 gtaggtatat aagccagcgt ctgagctaat aaccagaatg aggcacaata gcaaataatt
      361 gtgccaacag agggccataa ccgtgtaaac ccctctgaaa acttcattaa ggttgtacca
      421 atgacctctg caagtattgc gccaccaaga taaatataag ggttcatagc atattctttc
      481 ctgttcatac tggagagaat tgtactacag tttgaacaca actcaccgtt ttcatcctgg
      541 tgtatgtgta tccagggggc tctggcgtgg caagagataa aggcaggaac aataatgatc
      601 agaaggaacc ccaggctaac gaatgagttc actcttcctg ggagcaaagc gaagacaaaa
      661 catgttatcg cgtttacgga tcgtggatgg cagaaaacaa ccaggatcag gtatccattt
      721 taaaccagaa attgagtgag tttgccccat ccatgcccca cgctgtggga tcggatataa
      781 ttaaacaagc atagtcaaca ggttacacaa cctacacctg catggacatc acttcctgat
      841 acgccgccac cagcttatta cgcacctgaa tccccatttg catagaaact gaggcttttt
      901 gcatatcggt catcacatcg tttagcgcca cgccgggttc accgagggtg aatttttctg
      961 cctgcgtgcg ggcggctgtt tgtgtatcgc ttatacgatc gagcgcggcg tgcagctgcc
     1021 cggcaaaact aatggtcggt tgcggcagtg attcctgcgc acgcgcactc atcgccgtag
     1081 cctgtaactg gctgataacc ccttcaatcc cctgtatcgc tgacattttc gtctcccgga
     1141 taatttctgg tagcaaagcc taccagtaag tcaataagac aaaggcgcta aatagcaaca
     1201 aaaaaacggg tttattggcg gatagaaaaa aacgaaagca caaataatga gagcgtcaat
     1261 ttttcgagtt tgctgacccg ggagtgagtc ttgttccact ttgccaataa cgccgtccat
     1321 aatcagccac gaggtgcgcg atgaatgcga ctgcagtcca gacaaaatct cttgagtggc
     1381 ttaatcgcct gcgtgcgaat ccgaaaattc cattgattgt tgccggttcc gcagcagtgg
     1441 cggtcatggt cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca
     1501 atctttccga tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt
     1561 accgcttcag cgaagccagc ggcgctattg aagttccggc agataaagtt cacgaactgc
     1621 gtctgcgcct ggcacaacaa ggtttgccca aaggcggcgc ggtcggtttc gaactgctcg
     1681 atcaggaaaa gtttggtatc agccagttca gcgaacaggt gaattatcag cgggcgctgg
     1741 aaggcgagct ttctcgtacc atcgaaacta tcggcccggt aaaaggggcg cgcgtacatc
     1801 tggcaatgcc gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct gcatcggtga
     1861 cggtaaatct gttacccggc cgcgcactcg atgaagggca aattagcgcc attgtgcatc
     1921 tggtttccag cgccgttgct ggtctgccgc cgggaaacgt cactctggtg gatcagggcg
     1981 gacatctgtt aacccagtcc aacaccagcg ggcgcgatct taatgacgct cagttgaaat
     2041 atgccagcga tgtcgaaggc cgtattcagc ggcgtattga agcgatcctg tcgcctattg
     2101 ttggtaacgg taatattcac gcccaggtca cggcgcagct ggacttcgcc agtaaagaac
     2161 aaacggaaga acagtatcgc cctaacggtg atgaatctca ggcggcgctt cgttcacgcc
     2221 agcttaatga gagcgagcaa agcggttccg gttatccagg cggcgtaccg ggggcgttgt
     2281 cgaatcaacc ggcacctgcg aataacgcgc caatcagcac gcctccggca aatcaaaata
     2341 accgccagca gcaggcgagc accaccagca atagtgggcc gcgtagcaca cagcgtaatg
     2401 aaaccagtaa ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac gtgggcgatg
     2461 tgcaacgtct gtcagtcgcg gtagtggtga attacaaaac cttgccagac ggtaaaccgt
     2521 tgcctctcag caacgaacag atgaagcaaa ttgaagatct gacccgcgag gcgatgggct
     2581 tttctgaaaa acgcggtgac tcgctcaatg tcgttaactc gccgttcaat agtagtgagg
     2641 aaagcggcgg agaactgccg ttctggcaac aacaagcgtt tatcgatcag ttacttgctg
     2701 ccggtcgctg gttgctggta ctgctggtgg cgtggctgct gtggcgtaaa gcggtacgcc
     2761 cgcagctaac acgtcgcgct gaggcgatga aagctgtaca gcaacaggcg caggcccgtg
     2821 aggaagtgga agatgcggtg gaagtccgcc tgagcaaaga cgaacaactc caacaacggc
     2881 gcgctaacca acgactgggt gcagaagtca tgagccagcg tatccgtgaa atgtctgata
     2941 acgatccgcg cgtggtggcg ctggtcattc gccagtggat aaataacgat catgagtaac
     3001 ctgacaggca ccgataaaag cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca
     3061 gaggtgttca agcacctctc ccagcgcgaa gtgcaaactc tgagcgctgc aatggcgaac
     3121 gtcacgcaga tctccaacaa gcagctaacc gatgtgctgg cggagtttga gcaagaagct
     3181 gaacagtttg ccgcactgaa tatcaacgcc aacgattatc tgcgttcggt attggtcaaa
     3241 gctctgggtg aagaacgtgc cgccagcctg ctggaggata ttctcgaaac ccgcgatacc
     3301 gccagcggta ttgaaacgct caactttatg gagccgcaga gcgccgccga tctgattcgc
     3361 gatgagcatc cgcaaattat cgccaccatt ctggtgcatc tgaagcgcgc ccaggccgcc
     3421 gatattctgg cgttgttcga tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc
     3481 tttggcggcg tgcagccagc cgcactggcg gagctgaccg aagtactgaa tggcttgctc
     3541 gacggtcaga atctcaagcg cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc
     3601 aacctgatga aaactcagca ggaagaagcc gttattaccg ccgtgcgtga attcgacggc
     3661 gagctggcgc agaaaatcat cgacgagatg ttcctgttcg agaatctggt ggatgtcgac
     3721 gatcgcagca ttcagcgact gttgcaggaa gtggattccg aatcgctgtt gatcgcgctg
     3781 aaaggggccg agcagccact gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc
     3841 gatattctgc gcgacgatct cgccaaccgt ggtccggtgc gtctgtcgca ggtggaaaac
     3901 gaacagaaag cgattctgct gattgtgcgc cgccttgccg aaactggcga gatggtgatt
     3961 ggcagcggcg aggataccta tgtctgataa tctgccgtgg aaaacctgga tgccggacga
     4021 tctcgcgcca ccaccggcag agtttgtgcc aatggtcgag tcggaagaaa ccattattga
     4081 agaggccgaa cccagccttg agcagcaact ggcgcaactg caaatgcagg cccacgagca
     4141 gggttatcag gcggggattg ccgaaggtcg ccagcaaggt cacgaacagg gctatcagga
     4201 aggactggcc caggggctgg agcaaggtct ggcagaggcg aagtctcaac aagcgccaat
     4261 tcatgcccgg atgcagcaac tggtcagcga atttcaaact acccttgatg cgcttgatag
     4321 tgtgatagct tcgcgcctga tgcagatggc gctggaggca gcacgtcagg tcataggtca
     4381 gacgccaacg gtggataact cggcactgat caaacagatc caacagttgt tgcagcaaga
     4441 accgttattc agcgggaaac cacagctgcg cgtacacccg gatgatctgc aacgtgtgga
     4501 tgatatgctc ggcgcaactt taagtttgca tggctggcgc ttgcggggcg atcccaccct
     4561 ccatcctggc ggctgtaaag tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac
     4621 tcgctggcaa gaactctgcc gtctggcagc accaggagtg gtgtaatgac cacgcgcctg
     4681 actcgctggc taaccacgct ggataacttt gaagccaaaa tggcgcagtt gcctgcggta
     4741 cgtcgctacg ggcgattaac ccgcgctacc gggctggtgc tggaagccac cggattacaa
     4801 ttgccgctcg gcgcaacctg tgtcattgag cgccagaacg gcagcgaaac gcacgaagta
     4861 gaaagcgaag tcgttggctt taacggtcaa cggctgtttt taatgccgct ggaggaagtg
     4921 gaaggtgtcc tgcccggcgc gcgtgtatat gccaaaaaca tttcggcaga agggctgcaa
     4981 agcggcaagc agttgccgct cggtccggcg ttattaggtc gtgttctgga cggtagcggt
     5041 aaaccgctcg atggcctgcc cttccccgat acgacggaaa ccggtgcgct gattaccccg
     5101 ccatttaacc cgttgcaacg tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca
     5161 atcaatgccc tgcttaccgt cggacgcggg cagcgtatgg ggctgtttgc cgggtccggc
     5221 gttggtaaaa gtgtgctgtt ggggatgatg gcacgttaca cccgcgccga tgtcattgtc
     5281 gtgggtttga ttggtgaacg tgggcgcgaa gtaaaagatt ttattgagaa tatcctcggt
     5341 gccgaagggc gtgcacgctc agtggtgatt gccgctccgg cggatgtttc tccgctcctg
     5401 cgaatgcagg gtgccgccta tgccacgcgc attgccgaag attttcgcga tcgtggtcag
     5461 catgtgttgc tgattatgga ctccctcacc cgctacgcga tggcccagcg tgaaattgcg
     5521 ctggcgattg gcgaaccacc tgccactaaa ggttatccac cgtcggtgtt tgccaaatta
     5581 ccggcactgg tcgagcgtgc cggaaatggt attagcggcg gcggctcgat taccgcgttt
     5641 tataccgtgc tcaccgaagg cgatgaccag caggacccca ttgctgactc cgcgcgggca
     5701 atcctcgacg gtcacattgt gctgtctcgc cgactggcgg aagccgggca ctatccggct
     5761 atcgatattg aagcgtcgat cagtcgcgca atgacggcgt tgatcagtga gcaacattac
     5821 gcgcgagtgc gcaccttcaa acagctgttg tcgagttttc agcgtaaccg cgatctggtt
     5881 agcgtcggcg cgtatgccaa aggcagcgat ccgatgctcg ataaagccat cgccctgtgg
     5941 ccgcagctgg agggctattt gcaacaaggc atttttgaac gcgcggactg ggaagcgtct
     6001 ctccaggggc tggagcgtat tttcccgaca gtgtcataac ccaggagata acggcagatg
     6061 gcggaacatg gtgcgctggc gaccctgaaa gatctggcag aaaaagaggt agaggatgcc
     6121 gctcgcctgc tgggtgaaat gcgtcgcgga tgtcagcagg cggaagaaca gctcaaaatg
     6181 ctgatcgatt atcagaatga atatcgcaat aacctcaata gcgatatgag tgccgggatg
     6241 accagcaacc gctggatcaa ctatcagcag tttatccaga cgctggaaaa agccattact
     6301 cagcatcgcc agcaacttaa tcagtggacg cagaaagttg acattgccct gaacagctgg
     6361 cgagaaaaga aacaacgttt gcaggcctgg cagacactgc aggaacggca atccacggcg
     6421 gcactgcttg cagaaaaccg cctcgatcag aaaaagatgg atgagttcgc ccagcgcgcc
     6481 gccatgagga aacctgaatg attcgcttag cgcccttgat taccgccgac gttgacacca
     6541 ccacattgcc tggcggcaaa gccagcgatg ctgcacaaga ttttctcgcg ttgttgagcg
     6601 aagcattagc aggcgagaca acaaccgaca aagcggcccc ccagttgctg gtggcaacag
     6661 ataagcccac gacaaaaggc gagccgctga tcagcgatat tgtttccgac gcgcaacaag
     6721 cggatttact gatccctgtg gatgaaacac ttcctgtcat caacgacgaa caatccacat
     6781 caacaccgtt aaccaccgct cagacgatga cgttggctgc ggtggctgac aaaaatacga
     6841 caaaagacga aaaagcggat gatctgaatg aagacgtcac cgcaagcctg agtgcccttt
     6901 ttgcgatgtt gccgggtttt gacaatacgc ccaaagtgac tgatgcaccg tcaaccgtat
     6961 taccgacaga gaaaccaacg ctcttcacaa aactgacttc tgcgcaactc acaacagcac
     7021 aggctgatga cgcccccggc acaccagctc agccattaac accgctggta gcagaagccc
     7081 agagtaaagc ggaagtcatc agcacacctt caccggtgac cgctgccgcc agcccgctaa
     7141 tcactccaca ccagacacag ccactgccca ccgtcgccgc gcctgttttg agtgcaccgc
     7201 tgggttctca cgaatggcaa caatcattaa gccagcatat ttcgctgttc acccgccagg
     7261 ggcaacaaag tgcagagttg cgtctgcacc cacaggattt aggtgaagtg caaatttccc
     7321 tcaaagtgga tgataaccag gcgcagatcc agatgatttc accgcatcag cacgtacgcg
     7381 ccgccctgga agcagcgctg ccggtactgc gtacgcagct ggccgaaagt ggcattcagt
     7441 tagggcaaag caacatcagt ggcgaaagct ttagtggtca gcagcaggcc gcttcccagc
     7501 aacagcaaag ccaacgcaca gcaaaccatg aacctctggc gggggaagac gacgatacgc
     7561 ttccggttcc cgtctcttta caagggcgtg taacaggtaa cagcggcgtt gatattttcg
     7621 cctaacgtca gaggtagcac cgtaatccgc gtcttttccc cgctttgttg cgctcaagac
     7681 gcaggataat tagccgataa gcagtagcga cacaggaaga ccgcaacaca tgactgatta
     7741 cgcgataagc aagaaaagca agcgatcgct ttggatcccg attctggtat tcattaccct
     7801 cgcggcctgt gccagcgcag gttacagcta ctggcattcg catcaggttg ccgctgacga
     7861 caaagcgcag caacgcgtcg tgccctcccc ggtcttctat gcgctggata cctttacggt
     7921 caatttgggc gatgcggatc gcgtgcttta tatcggcata accctgcgac tgaaagatga
     7981 agctacccgc tcgcggctga gtgagtattt gccggaagtc cgtagccgtt tgctgttact
     8041 gttttcgcgt caggatgctg ccgtactggc gacagaagaa ggcaagaaaa acctgattgc
     8101 cgagattaaa accacacttt ccaccccgct tgttgccggg caaccgaaac aggatgtcac
     8161 cgacgtgctg tataccgctt ttattctgcg ataacgacat gggcgatagt attctttctc
     8221 aagctgaaat tgatgcgctg ttgaatggcg acagcgaagt caaagacgaa ccgacagcca
     8281 gtgttagcgg cgaaagtgac attcgtccgt acgatccgaa tacccaacga cgggttgtgc
     8341 gcgaacgttt gcaggcgctg gaaatcatta atgagcgctt tgctcgccat tttcgtatgg
     8401 ggctgttcaa cctgctgcgc cgtagcccgg atattaccgt cggggccatc cgcattcagc
     8461 cgtaccatga atttgcccgc aacctgccgg tgccgaccaa cctgaacctt attcatctga
     8521 aaccgctgcg cggcacaggg ctggtggtgt tctcgccgag tctggtgttt atcgccgtgg
     8581 ataacctgtt tggcggcgat ggacgcttcc cgaccaaagt ggaaggccgc gagtttaccc
     8641 ataccgaaca gcgcgtcatc aaccgcatgt tgaaactggc gcttgaaggc tatagcgacg
     8701 catggaaggc gattaatccg ctggaagttg agtacgtgcg ttcggaaatg caggtgaaat
     8761 ttaccaatat caccacctcg ccaaacgaca ttgtggttaa cacgccgttc catgtggaga
     8821 ttggcaacct gaccggcgaa tttaatatct gcctgccatt cagcatgatc gagccgctac
     8881 gggaattgtt ggttaacccg ccgctggaaa actcgcgtaa tgaagatcag aactggcgcg
     8941 ataacctggt gcgccaggtg cagcattcac agctggaact ggtggccaac tttgccgata
     9001 tctcgctacg cctgtcgcag attttaaaac tgaaacccgg cgacgtcctg ccgatagaaa
     9061 aacccgatcg catcatcgcc catgttgacg gcgtcccggt tctgaccagt cagtacggca
     9121 ctctcaacgg tcagtatgcg ttacggatag aacatttgat taacccgatt ttaaattctc
     9181 tgaacgagga acagcccaaa tgagtgacat gaataatccg gccgatgaca acaacggcgc
     9241 aatggacgat ctgtgggctg aagcgttgag cgagcaaaaa tcaaccagcg gaaaaagcgc
     9301 tgccgacgcg gtgttccagc aatttggcgg tggtgatgtc agcggaacgt tgcaggatat
     9361 cgacctgatt atggatattc cggtcaagct gaccgtcgag ctgggccgta cgcggatgac
     9421 catcaaagag ctgttgcgtc tgacgcaagg gtcagtcgtg gcgctggacg gtctggcggg
     9481 cgaaccgctg gatattctga tcaacggtta tttaatcgcc cagggcgaag tggtggttgt
     9541 tgccgataaa tatggcgtgc ggatcaccga tatcattacc ccgtccgagc ggatgcgccg
     9601 cctgagccgt tagtgatgaa taaccatgct actgtgcaat cttccgcgcc ggtttctgct
     9661 gagccactgc tgcaggtgag cggcgcactc atcgccatta ttgccctgat cctcgctgct
     9721 gcctggctgg taaaacggct gggatttgcc ccgaaacgca ctggcgttaa cggtctgaaa
     9781 attagcgcca gtgcttcgct gggcgcgcgt gaacgggtcg tggtggtcga tgtggaagat
     9841 gcacggctgg tgctcggcgt taccgctggg caaatcaatc tgctgcataa acttccccct
     9901 tcagcaccaa cggaagagat accgcagact gattttcagt cggtcatgaa aaatttgctt
     9961 aagcgtggcg ggagatcctg atgcgtcgtt tgttttctgt cgcaccggtc cttctctggc
    10021 tgattacgcc cctcgccttc gcgcaactgc cgggtatcac cagccagccg ctacctggcg
    10081 gtggacaaag ctggtcgctc ccggtgcaga cgctggtgtt catcacctcg ttgacgttca
    10141 ttccagcgat tttactgatg atgaccagtt tcacccgcat cattattgtt tttggtttat
    10201 tgcgtaacgc gctgggaacg ccctccgcgc cacctaacca ggtattgctg gggctggcac
    10261 tgtttttgac cttttttatt atgtcgccag tgatcgacaa aatttatgtc gatgcgtacc
    10321 agccattcag cgaagagaaa atctcaatgc aggaggcgct ggaaaaaggg gcgcagccgc
    10381 tgcgtgagtt tatgctgcgt cagacccgtg aggcagattt agggttgttt gccagactgg
    10441 cgaataccgg cccgttgcag gggcctgaag ctgtgccgat gcgcattttg ctcccggcct
    10501 acgtgaccag cgagttgaaa accgcatttc agataggctt cacgattttc atcccttttt
    10561 tgattatcga cctggtgatt gccagcgtgt tgatggcatt ggggatgatg atggttcccc
    10621 ccgccaccat tgctctgccc tttaaactga tgctgtttgt actggtggat ggctggcaat
    10681 tgctggtcgg ttcgctggcg cagagctttt acagctagag aggcaaaatg acacctgaat
    10741 cggtcatgat gatgggaact gaagcgatga aagtcgcgct ggcactggca gccccgctgt
    10801 tgctggtggc gctggtcacg ggccttatca tcagtatttt gcaggcagcc acgcagatta
    10861 acgaaatgac gctgtcgttt attccgaaaa tcatcgccgt atttatcgcc attattattg
    10921 ccggaccgtg gatgctcaat ctgttgctgg attacgtccg caccttgttc actaacctgc
    10981 cgtatatcat cgggtagccc gtacaatgat gcaggtgaca agcgatcaat ggctttcctg
    11041 gttaagcctg tacttctggc cgttactgcg cgtgctggcg ctcatttcta ccgcgccaat
    11101 tctgagcgaa cgcagcgtgc cgaaacgggt aaaactgggt ctggcaatga tgatcacgtt
    11161 cgccattgcc ccatcattac ctgccaacga tgttcctgtt ttttcgttct ttgctctgtg
    11221 gctggccgtg cagcagatcc tgatcggcat tgcgcttggt tttaccatgc aatttgcctt
    11281 tgccgctgtg cgaaccgctg gcgaaattat cggtctgcaa atggggctgt catttgcgac
    11341 atttgtcgat cctggcagcc atctcaatat gcccgtttta gcgcgcatca tggatatgct
    11401 ggcattactg ctgttcctga catttaacgg tcatttatgg ttgatttcac tgctggtcga
    11461 tacctttcac accctgccga ttggtagcga accgttgaac agcaatgcgt ttctggcact
    11521 catcaaagca gggagtttga ttttccttaa cgggctgatg ctggcgttac cgctcattac
    11581 tctgttgctg accctgaatc tggcattagg tttactgaat cgtatggccc cgcaattatc
    11641 catttttgtt attggatttc cattaactct gactgtcggc atctctttaa tggcggcatt
    11701 aatgccgtta attgcgcctt tttgcgaaca tttattcagt gaaattttta atttgctggc
    11761 tgatattatt agtgaattac cattaatata attccgtaac gtttatcatg ctaccctaag
    11821 gattatccga aaaataatat ctgcgaacat cttccaggat actcctgtag cgaaatattt
    11881 gttttaagct cactcacata tcgcaacatt tactttactt taagacaatt ccaggcaaat
    11941 tatacaacac tttacgggat agtaagtccg cctgaaaaat tgcgagagtg gcgcattagg
    12001 tgacccatgt tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac
    12061 gcattgagtg agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac
    12121 taggtttaac cgggtatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg
    12181 aaaccgttga tgaccttgcc atagcttgtg attcacagcg cccttcagtg gtgtttatta
    12241 atgaggactg tttcatccac gatgcttcta acagtcagcg catcaagcac atcattaatc
    12301 aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat
    12361 atctattggt cagaaaaaat ttattgatca gttctaaatc gattaaaccg gaatctctcg
    12421 acgatatcct tggcgatatt ctgaaaaaag agacaacgat aacctcgttt ttaaatatgc
    12481 cgacgttatc attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg
    12541 gaaccattca aatctccgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag
    12601 gtaatattaa acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac
    12661 tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg
    12721 gtttcccacc agtcaggctg aataagatta ctctgctttc tcaacaaaga taccgtcctg
    12781 atgccctgct tcattaaaga accagatgcc gagcgggtag tcttccagcg aaaccaggta
    12841 cattgtgcct tcactaaact cctcaactgc cagtaccacg ccaggacgac gcggaccgcc
    12901 atccgttttg actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc
    12961 cagtgtagaa caatttcgtt tttctggcag cgccaggcgc gcgcgagtgc tgattttctc
    13021 gacggtctat acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa
    13081 cggttgcgag gaaagatgat gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag
    13141 gtcagcgtcg atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt
    13201 catcgcagcc agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg
    13261 cgtgaattgg cggatgcctt cgaactggat atccatgact tcagcgtctc tgaagtgaat
    13321 cgttgaatgc acaataaaaa aatcccgacc ctgagggggt cgggatgaaa cttgcttaag
    13381 caagaagcac ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatctg
    13441 caattttttt cgctgacaag aaaatattcg cgccatgaat gattaacgct acttatgaat
    13501 agaaatgtga cgcgcgtcac cccttggtgg ctctggtgtc aacgggatcg ctcccgaaaa
    13561 aatcatcttt gattatttgt gctgttttga gaatcatcct ggtaagggga gtactgcgcg
    13621 accatgtttt caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg
    13681 gacagtcata gttatgactg gcaaccggca gccccctggc tcagctgttt acacgaagcc
    13741 aatattcccg tcattctctg tagcagtaaa acttcagcgg aaatgctgta cttgcaaaaa
    13801 atgttggggc tacaaggctt accgctgatt gcagagaatg gcgcagtgat ccagcttgct
    13861 gaacaatggc aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa
    13921 atctgccagg ttttaaatac gctacgcgag aaagaacatt ttaaattcac gacttttgat
    13981 gatgtcgacg atgcaaccat tgccgaatgg acgggattaa gccgtagcca ggcggcgctg
    14041 acgcagctgc atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca
    14101 caatttatcg ctcgtctgaa cgaactgggc ttgcagttta tgcagggtgc gcgcttctgg
    14161 cacgtactgg atgcctctgc cggaaaagat caggctgcca actggattat cgcgacctat
    14221 caacaattgt caggcagacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg
    14281 cctttactgg aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggtc
    14341 catctgcatg atgaggatcc ggcccgcgtc tggcgaacgc agcgtgaagg accggaaggc
    14401 tggcgtgaag ggctggacca ttttttcccc gcccgttaag cgttatcgct cgcgaatacc
    14461 cgattgcgcc cggcctgttt tgcgaggtaa agccgacggt cggccaatga ctggagttgt
    14521 tcaaaatcat aatcaccggt ttcctcgctg ctgcttaccc ccagcgaggc actaatgcgt
    14581 atcgttgtac tcttggcgat cagcatctct ttttcattta acttcaggcg aatacgttct
    14641 gcgacctccg cagcctgcgt cagattcgcg cctggcagaa tcacacaaaa ctcctcacca
    14701 ccgacccgcc cggcaatgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga
    14761 gaaagaacac ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca
    14821 agatcgacct gaatgacaga aaaaggatgt tggtgcgtgt gacacaattt agcgagcgga
    14881 caggcttttt cgaacaatgc gccacggtta tataaacgcg ttaaggtgtc gtgccacgcc
    14941 tgccactgca acgagctttg cagaacatac atgttgctga ccatccggcg aatcacatac
    15001 caggagagga gtaacatgct ggtaaagagc gcccacagca gggttaatgc aatgctgata
    15061 ctgccgaaat cgccgcgcac gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg
    15121 aaatggtcca gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg
    15181 tcatgttcca ttgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt
    15241 ggatggtcag gatttgaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca
    15301 ccatcgaggt ttttatcgat ggcgtttctt aaaaattgct gcatagtacg cacgggaata
    15361 ctcatcccca gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc
    15421 tgcggttcag tattgctggc gtgttccggt tgcgaagtga accagcgtac cgcgcggtga
    15481 cgattttctc gttgtgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc
    15541 gttggtacat tgcgcgtaaa caaggtcggc agcgtcgaaa cgtaaaatcc agcacgcgag
    15601 acatacacag cctgttcaac catcgacgag gagttgtgcg ccagtcgcag caagtaacca
    15661 acttccagtg cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggagattc
    15721 ccctcgctga ctaatgcatc cgatacgcca ttgactggca gggtgcgccg tcggttgagt
    15781 tcgatttgcc aggcgtgctc gtcgcgatgc tgttcgaact cggttacggc attacgcaga
    15841 gaggtgaaat ccagtggcgc cacgagagct tcgcgcatgc cattacgcaa aaagatcagt
    15901 ttgtcgacgt tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta
    15961 cgctggctgg atatataggc atcttccagc accaccactt cacgccaggt gagcaaggtg
    16021 gaaaaaagca ataccacgat aaagcagaga ttaacgacat gaccaggccc caggcggcgt
    16081 gcgagttttt tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact
    16141 cccaacctgc ttttttatga ttctggcata acccggcgat agcgtaccgc aaaacagcga
    16201 taattttata atggtggcat gataagacgc gactatcatt cattgcaggc attgggacac
    16261 caggccggat gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac
    16321 gactcccctc ccccggaaca agatcggttt catgaaaagc ttcccgcttg accccgtaac
    16381 cgtcatacca acgacactca accataccgc tggagtatcc agtgacaatc atccgcgggc
    16441 cgccctcttt aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc
    16501 gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc cagtgcgagt
    16561 attaatgtgc ctgaccgcgc attttcgcta ctgctttcac tcccagcacc acgatgccgc
    16621 cgatgataaa tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac
    16681 tttgctgccc ggcgatatgt tcaatagcgt gatgcagcgg cgcaatacca tgtaccacaa
    16741 tcccgccgcc gacgaggaac atcgccagcg tgccgacaat cgatagcgct ttcatcagcc
    16801 agggcgcgat aatcaataac cctttaccta atgcctgcat cagcgcgctg gatttttccg
    16861 ccagccaata ccccaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca
    16921 cggtcactac cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggtgcctctg
    16981 ccacaatccc cagcgtaatg gcgacgattt ccgcagacaa aataaaatcg gtacgaatcg
    17041 cccctttaat tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac
    17101 gctgctggct ctgcgctgga tcttctttat gtttacgcgc ctccagcata tgcagcactt
    17161 tctctacccc ttcaaagcag agaaacgcgc caccaatcat caacagtggc gtaatcgccc
    17221 acgggataaa cgcactgatg atcaacgcca gcggcaccag aatcacttta ttgatcagcg
    17281 atcctttcgc cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt
    17341 gttgcgcatt gagcgataaa tcatccccta atacaccggc ggttttcttc gccgccagtt
    17401 tgcccatcac ggagatatcg tccagcagtg tggcgatatc atcgagcaac gtcagtaaac
    17461 tactcccggc caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca
    17521 aaaacggcag ctctgatact tgcttcacag gtcaacattt atttaacaat caagtgcaat
    17581 tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccgc
    17641 cgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt
    17701 atatcatttg gggctcaacc tattttgtca ttcggattgg cgtggaaagc tggcctccgt
    17761 taatgatggc gggcgttcga ttcctggcag ccggtatttt attgctggca tttttgctac
    17821 tgcgcggaca caaacttccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt
    17881 tattgctggc tgtcggtaat ggcatggtga cggttgccga acatcaaaat gttccttccg
    17941 gcatcgccgc cgtagtggtt gcaaccgtgc ccctctttac cctgtgcttc agtcgcctgt
    18001 ttggcattaa aacgcgcaaa ctggaatggg tgggtatcgc cattgggctt gccggaatca
    18061 tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa
    18121 tcggctcgat tagctgggcg tttggctcag tttatggctc gcgcattacc ttacctgtag
    18181 ggatgatggc gggtgcgatt gagatgctgg cggcaggcgt ggtgttaatg atcgcgtcga
    18241 tgattgcggg tgaaaaactg acggcgctcc cttccctgtc aggcttcctt gcagtcggct
    18301 atctggcgct gtttggttcg attatcgcca ttaacgctta tatgtattta atccgtaatg
    18361 tcagtccggc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg
    18421 gtacgggact gggtggcgaa acgctgtcga agattgaatg gctggcgctc ggcgtaattg
    18481 ttttcgcggt ggtactggtc acgttgggaa aatatctctt cccggccaaa cccgttgtcg
    18541 cgccagttat tcaggatgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg
    18601 ctggcacctt cgccgcagat ccactcttcc agacgctcgg taagcgccgc atccgtcagc
    18661 ttcttgcgcc cacgtaacgc acactcccag acaatcagta cgcgccagcc gagctcctgc
    18721 aagcgactga tatcgcggcg atcgcgttca acatttttac ctatcttctc cagccagaac
    18781 tcggttcgag tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg
    18841 tgggtaaata tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg
    18901 gcgtcctgaa cgcgaaacgc aaggccctgc ccggttaaca aactggcgag gcgcttctca
    18961 atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgggtggc cttatcgtga
    19021 acgtcgacca tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc
    19081 aagcagtttt gccaccgcgg caaagaccgg cacgaccacc gagttaccga actggcgata
    19141 ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat
    19201 taagcgcgcg cattcacgag gtgttaaccg acgtggacga tgttgctgat tcagcggatc
    19261 gtcaaagtct ttctcacccg tggccatatc ccagccgcgg tcgattaaaa tttccgcgcc
    19321 atctttgtaa taacgagcag acagcgtacg ggtgacgctt tgcggattgt tcggataaac
    19381 cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta
    19441 cttccacagc accggcgtca ggatatattt cgcctcaacc atcgggtcca gcagctgcgc
    19501 cagcgtcact cgctgtgcag ggaaacattc gctgatatca cgcagagtaa aatcggcttt
    19561 cagattcaga tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gcggcaggaa
    19621 atgtttacca tcgatgattt tcggatcgtc cggcccgtta tcttctgcat cagccacgtc
    19681 atagcccagt tcgtccagcg tctgcatgat gatgcggaac gtttttccct tatcgtgact
    19741 cttcaggttt ttgacgtttt cgagcacaaa catcgccgga cgacgcgcgt cgataatgcg
    19801 caccacatca aagaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag
    19861 cgagttcttt ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa
    19921 aacatcgtgt tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc
    19981 ttcttgatgg ctgagggtga tgtcgcggat atcctcatta aaatgatgcg tcgccgggtc
    20041 gcaataatgg ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac
    20101 gcactgtcca ccaatcgatt caaaaccacg acggatgccg ccaattccgg caaatagatc
    20161 gataaagcga aacgcataat gcggatgatg ttccggtggt ttgggtaaca acgtttgcag
    20221 atgggcaaac tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt
    20281 taaaattgcc gcgctccagt gattctcacc cacaccatta agctgcgcca ccagcgtttt
    20341 aacatcataa atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta
    20401 tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac
    20461 aggttaacat aattactgcc gtttagggcc acgcgctcag ccagcaggtg aaaacgcatc
    20521 tatcaccttg tgatcgatac cttcattctc ccccgccagt tcggcaccga gcttcgccat
    20581 aaactcgact aaaaattgcg cgttgtgctg tgccagctgc ttgcctcttg cggtttgcat
    20641 ggtttgcggt aatttcagta actttgtctg gaaatgatcc agcgcgtagc gtttatcatc
    20701 aagcgggcga tgctgtgcga acggatcttc gccatcaaac agcgccaagc ccaatgcccc
    20761 tgaaacggca aatacgcgcg ccaggccaat cgcccccagg gcttccagcc gatccgcatc
    20821 ctggacgatt ttagcttccg ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc
    20881 aatggcatga caaacggcct caattttctc cgccggaaat tgcacgaact cttcacggag
    20941 caggcgacgc gtctcttctg ccgccaggat tgaagaacgc tgccgttgcg gatgattttt
    21001 cgccaggctc acgatatcgt gaaaataaca ggcggttaaa atcaccagca tatcgacatc
    21061 gtcatctgcc gccagtttct gtgccgtagc ccagacacgg cgaaaatgac acaggtcatg
    21121 cgccgcgtcc tgatgttgat gatggttttt caaccagttc tcgaactgtg cctgccagtg
    21181 ttgtaactcc atacgcgctc cgttgccaaa gagtcgcaac cttagcagtt tttattcata
    21241 gcgtctgcaa cgtgcgggac gaggtcgggt gtaccatgca atgccgccta acaaggcacc
    21301 aaccagggcc ataaacagaa atccgaccaa tgctcctaac cacttccccg cggtagatcc
    21361 taaatcactt tcaaatcccg acacgggggc atcggggatc cggctcatca cccagatgta
    21421 tcgcgccatt gcccagacga aatagccaca ccatgtatag aaagcccaca aagccaattt
    21481 gccgccaggg ctgcgagaga gtctctcttc catctttgtc gaaccgtatt ccattttgag
    21541 ataacccgga aaattcagac ttagaactat tatgtgataa aaatcacatt tttccacatt
    21601 ggatgagcca gtcaagactc aatcattttt tgaacaaata gataaatttt gcgcaacggc
    21661 ggtgaataca ggaaaaatcg atttgaacga tgttatttat ctttgatttc
//
LOCUS       DADSZD010000072        21633 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.072, whole genome shotgun sequence.
ACCESSION   DADSZD010000072 DADSZD010000000
VERSION     DADSZD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.072"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..126
                     /locus_tag="J1Z28_002420"
     CDS             <1..126
                     /locus_tag="J1Z28_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBA7717248.1"
                     /translation="THFRLVIRNAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            217..1035
                     /locus_tag="J1Z28_002421"
     CDS             217..1035
                     /locus_tag="J1Z28_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA7717249.1"
                     /translation="MRLASRFGYVNQIRRERPLTREELMYHVPSIFAEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDSVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPSRQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRTIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1127..1612
                     /locus_tag="J1Z28_002422"
     CDS             1127..1612
                     /locus_tag="J1Z28_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA7717250.1"
                     /translation="MTTVTHNSTTPSVSVTAASGNNPPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1628..2104
                     /locus_tag="J1Z28_002423"
     CDS             1628..2104
                     /locus_tag="J1Z28_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBA7717251.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            2173..2394
                     /locus_tag="J1Z28_002424"
     CDS             2173..2394
                     /locus_tag="J1Z28_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA7717252.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2468..2836
                     /locus_tag="J1Z28_002425"
     CDS             2468..2836
                     /locus_tag="J1Z28_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBA7717253.1"
                     /translation="MSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPETKT"
     gene            2926..3303
                     /locus_tag="J1Z28_002426"
     CDS             2926..3303
                     /locus_tag="J1Z28_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBA7717254.1"
                     /translation="MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            3300..3788
                     /locus_tag="J1Z28_002427"
     CDS             3300..3788
                     /locus_tag="J1Z28_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717255.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3800..3997
                     /locus_tag="J1Z28_002428"
     CDS             3800..3997
                     /locus_tag="J1Z28_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBA7717256.1"
                     /translation="MKSLITETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            4082..4924
                     /locus_tag="J1Z28_002429"
     CDS             4082..4924
                     /locus_tag="J1Z28_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBA7717257.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(5204..5740)
                     /locus_tag="J1Z28_002430"
     CDS             complement(5204..5740)
                     /locus_tag="J1Z28_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBA7717258.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLTQHLAGRSPREKGMLLAAV
                     VFLFSAGYYVLIWQPLSERIEQQETMLQQLVAMNARLKSTAPDIVAARKSGTTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIEPVVFNDLLNWLKALDEKYALRVIQIDVS
                     AGEKPGMVNVQRLEFGRG"
     gene            complement(5742..6920)
                     /gene="gspL"
                     /locus_tag="J1Z28_002431"
     CDS             complement(5742..6920)
                     /gene="gspL"
                     /locus_tag="J1Z28_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA7717259.1"
                     /translation="MSSMLEIFFPLCAADPIRWQRYTPDVEHGIWPDVADERLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVPRKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQANGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPISLTVVGIAPEELSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALVVERGVTLWSVSEQVAQSRTQA
                     EKQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLTDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALAADYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(6917..7894)
                     /gene="gspK"
                     /locus_tag="J1Z28_002432"
     CDS             complement(6917..7894)
                     /gene="gspK"
                     /locus_tag="J1Z28_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBA7717260.1"
                     /translation="MITSPPKRGMALVVVLVLLAVIMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMARQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(7891..8496)
                     /gene="gspJ"
                     /locus_tag="J1Z28_002433"
     CDS             complement(7891..8496)
                     /gene="gspJ"
                     /locus_tag="J1Z28_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBA7717261.1"
                     /translation="MRRARAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLNHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHGGYLERLAWPLTDAAGSVKPTTQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRITLHSPQWGEIERIWLLRGPQLS"
     gene            complement(8493..8864)
                     /gene="gspI"
                     /locus_tag="J1Z28_002434"
     CDS             complement(8493..8864)
                     /gene="gspI"
                     /locus_tag="J1Z28_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBA7717262.1"
                     /translation="MKRGFTLLEVMLALAIFALAAMAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTAQLYLMTREQRAVRHQGESDMAGSRWYWRTTPLNTGNVLLQAVDIEVSLH
                     DDFSPVIQSRRAWFSAVGGQQ"
     gene            complement(8861..9424)
                     /gene="gspH"
                     /locus_tag="J1Z28_002435"
     CDS             complement(8861..9424)
                     /gene="gspH"
                     /locus_tag="J1Z28_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBA7717263.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATASEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRHGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(9428..9883)
                     /gene="gspG"
                     /locus_tag="J1Z28_002436"
     CDS             complement(9428..9883)
                     /gene="gspG"
                     /locus_tag="J1Z28_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBA7717264.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADARNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(9900..11123)
                     /gene="gspF"
                     /locus_tag="J1Z28_002437"
     CDS             complement(9900..11123)
                     /gene="gspF"
                     /locus_tag="J1Z28_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBA7717265.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAM
                     SDALQASGVYWLAGLLGLLVLGQRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALADLRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(11123..12616)
                     /gene="gspE"
                     /locus_tag="J1Z28_002438"
     CDS             complement(11123..12616)
                     /gene="gspE"
                     /locus_tag="J1Z28_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBA7717266.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSHELTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCS
                     DCRQAAPATDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(12616..14676)
                     /gene="gspD"
                     /locus_tag="J1Z28_002439"
     CDS             complement(12616..14676)
                     /gene="gspD"
                     /locus_tag="J1Z28_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBA7717267.1"
                     /translation="MFWRDMTLSIWRKKTTGLKTKKRLLPLMLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWGSKDAGLM
                     QFANGTQIPIGTLGAAISAAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPILPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(14706..15665)
                     /gene="gspC"
                     /locus_tag="J1Z28_002440"
     CDS             complement(14706..15665)
                     /gene="gspC"
                     /locus_tag="J1Z28_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBA7717268.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHILSQRQSLNTDKEHLRKMVRGM
                     FWLMLLIISAKMAYSLWRYFSFSAEYTAVSASANKPLRADAKPFDKNDVQLVSQQNWF
                     GKYQPVAAPVKQPESAPVAETRLNVVLRGIAFSARPGAVIEEGGKQQVYLQGERLGSH
                     NAVIEEINRDHVMLRYQGKMERLSLAEEERPPVAVTSKKAASDEAKQAVAEPVVSAPV
                     EIPAAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFREGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARYDISIALR"
     gene            complement(15683..16093)
                     /gene="gspS2"
                     /locus_tag="J1Z28_002441"
     CDS             complement(15683..16093)
                     /gene="gspS2"
                     /locus_tag="J1Z28_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HBA7717269.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGSTVKMTIISESGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(16159..16968)
                     /locus_tag="J1Z28_002442"
     CDS             complement(16159..16968)
                     /locus_tag="J1Z28_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA7717270.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWGLAVMILSAWLIAASIIDLDNQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGDW
                     VGPLSLPNVALIASCCGLIYAVITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(17163..>21633)
                     /gene="sslE"
                     /locus_tag="J1Z28_002443"
     CDS             complement(17163..>21633)
                     /gene="sslE"
                     /locus_tag="J1Z28_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HBA7717271.1"
                     /translation="SSDTPSVDSGSGTLPEVKPDPTPTPEPTPEPTPDPEPTPDPTPD
                     PEPTPEPEPEPVPTKTGYLTLGGSQRVTGATCNGESSDGFTFTPGNTVSCVVGSTTIA
                     TFNTQSEAARSLRAVDKVSFSLEDAQELANSENKKTNAISLVTSSDSCPADAEQLCLT
                     FSSVVDRARFEKLYKQIDLATDNFSKLVNEEVENNAATDKAPSTHTSTVVPVTTEGTK
                     PDLNASFVSANAEQFYQYQPTEIILSEGQLVDSLGNGVAGVDYYTNSGRGVTDENGKF
                     SFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYSTTGQNNTRV
                     VPDDVRKVFAEYPNVINEIINLSLSNGATLDEGDQNVVLPNEFIEQFKTGQAKEIDTA
                     ICAKTNGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYQSVSKFHVFHDSTNFYGS
                     TGNARGQAVVNISNVAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAPSIVQPENV
                     TRDTATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPNGYSWGGGVNSKGECTLSGDS
                     DDMKHFMQNVLRYLSNDIWQPNTKSIMTVGTNLENVYFKKAGQVLGNSAPFAFHEDFT
                     GITVKQLTSYGDLNPEEIPLLILNGFEYVTQWSGDPYAVPLRADTSKPKLTQQDVTDL
                     IAYLNKGGSVLIMENVMSNLKEESASSFVRLLDAAGLSMALNKSVVNNDPQGYPDRVR
                     QRRATGIWVYERYPAADGAQPPYTIDPNTGEVTWKYQQDNKPDDKPKLEVASWQEEVE
                     GKQVTRYAFIDEAEYTTEESLEAAKAKIFEKFPGLQECKDSTYHYEINCLERRPGTDV
                     PVTGGMYVPRYTQLNLDADTAKAMVQAADLGTNIQRLYQHELYFRTKGSKGERLNSVD
                     LERLYQNMSVWLWNDTKYRYEEGKEDELGFKTFTEFLNCYANDAYAGGTKCSADLKKS
                     LVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVEKYPGSVSA
                     KGESVTENISLYSNPTKWFAGNMQSTGLWAPAQQDVTIKSSASVPVTVTVALADDLTG
                     REKHEVALNRPPRVTKTYTLEANGEVTFKVPYGGLIYIKGDSKDDVSANFTFTGVVKA
                     PFYKDGEWKNDLDSPAPLGELESASFVYTTPKKNLEASNFTGGVAEFAKDLDTFASSM
                     NDFYGRNETSGNHRMFTYEALTGHKHRFANDVQISIGDAHSGYPVMNSSFSTNSTTLP
                     TTPLNDWLIWHEVGHNAAETPLNVPGATEVANNVLALYMQDRYLGKMNRVADDITVAP
                     EYLDESNGQAWARGGAGDRLLMYAQLKEWAEKNFDITKWYPDGKLPAFYSEREGMKGW
                     NLFQLMHRKARGDDVGNSTFGGKNYCAESNGNAADTLMLCASWVAQTDLSEFFKKWNP
                     GANAYQLPGASEMSFEGGVSQSAYNTLAAMHLSKPEKGPETINKVTEYSMPAE"
BASE COUNT         4877 a         6149 c         5460 g         5147 t
ORIGIN      
        1 actcatttcc ggctggtgat ccgcaacgct gaaggccagt tgcgctggcg gtgctggaat
       61 tttgaacctg atgccggaaa acagctaaat ccgtatctcg ccagtgaggg aattctcagg
      121 caataaacgt cattcacttg atccatcagg ccgcgtcttc tccggaagac gcggcttttt
      181 catttatacc gcttaatcat tcataaggag ccaattatgc gattagccag ccgttttggt
      241 tatgtaaatc agatccgccg ggagcgcccg ctgacacgcg aagaactgat gtaccacgtc
      301 ccgagtattt ttgcggaaga ccggcacacc tcccgcagtg aacggtatgc gtacattccc
      361 accatcaccg tcctagaaaa tctgcagcgg gaaggctttc agccgttctt cgcctgccag
      421 acccgtgtgc gcgaccagag ccgccgggaa tataccaaac atatgctgcg tctgcgacgg
      481 gccggacaga taaccggtca gcatgtgcct gaaattattc tgctcaactc ccatgacggt
      541 tcatccagct accagatgtt acccggatat tttcgtgcca tctgcaccaa tggcctagtc
      601 tgcggtcagt cgctgggaga agtccgggtg ccacaccggg gaaacgtggt ggacagtgtg
      661 attgaaggtg cttacgaggt ggtgggggtt tttgaccgga ttgaagaaaa gcgtgacgcc
      721 atgcagtcgc tggtcctgcc gccaccgtca cgccaggcgc tggcacaggc ggcactgact
      781 taccgttatg gtgacgaaca tcagcccgtc accaccgccg acattctgac gccacgacgc
      841 cgggaggatt acggtaagga cctgtggagt gcttatcaga ccatccagga gaatatgctg
      901 aaaggcggga tttccggtcg cagtgccaga ggaaaacgta tccatacccg taccattcac
      961 agcatcgaca ccgacattaa gctcaaccgg gcgttgtggg tgatggcaga aacactgctg
     1021 gagagcatgc gctgatgcct tttccctgaa agcgcagtcc tgactcactt ccgtccctgc
     1081 ctccggacat tcttccattc atttactttt tataaggaat aatctcatga caaccgttac
     1141 gcataattcc accacacctt ctgtttccgt aaccgctgca tcagggaata acccacctca
     1201 gctggttgct actctcgtcc ctgatgaaca gcgcatcagc ttctggccgc agcattttgg
     1261 cctcattcca cagtgggtga ccctggagcc ccgtgtcttc ggctggatgg accgtctgtg
     1321 cgaagactac tgtggtggta tctggaatct gtacaccctg aacaacggcg gggcatttat
     1381 ggcacccgaa ccggatgacg atgatgacga aacatgggta ctgttcaatg ccatgaacgg
     1441 taaccgcgct gaaatgagcc cggaagccgc cggtattgct gcctgtctga tgacgtacag
     1501 ccatcatgcc tgtcgtacgg agaattatgc catgacggtc cattattacc ggttgcggga
     1561 ttacgccctg cagcatccgg aatgcagcgc cattatgcgc attatcgact gaatggaggc
     1621 agggacaatg cagcagcttt cctttctgcc cggagagatg acgcccggcg agcgcagtct
     1681 cattctgcgg gccctgaaaa ccctggaccg ccatcttcat gaacccggcg tggccttcac
     1741 ctccacccgt gcggcacggg aatggctgat tctgaacatg gcgggactgg agcgggaaga
     1801 gttccgggtg ctgtatctga acaaccagaa tcagctgatt gccggtgaaa ccctcttcac
     1861 cggcaccatc aaccgcacgg aagttcatcc ccgggaagtg attaaacgcg ccctgtatca
     1921 caatgccgca gccgtggtgc tggcacacaa tcacccgtcc ggtgaagtca cccccagtaa
     1981 ggcagaccgc cttatcacgg aacgtctggt acaggcactg gccctggtgg atatccgggt
     2041 accggaccat ctgatagttg gtggcagtca ggttttctct tttgcggaac acggtctgct
     2101 ttaacccgtc accgtcacaa ccacatcaca cctgttttca cttttatctt ctgtcttcag
     2161 aggtatcaca ctatgaaaat catcacccgt ggtgaagcca tgcgtattca ccgtcagcat
     2221 ccggcatccc gtctttttcc gttctgcacc ggtaagtacc gctggcacgg cagtgctgag
     2281 acgtataccg gtcgtgaagt acaggatatt cccggtgtgc tggccgtgtt tgctgaacgc
     2341 cgtaaggaca gttttggccc gtatgtccgg ctgatgagcg tcactctgaa ctgagtcagg
     2401 agaaatgatg agccgaatta tcgctgccac cggaccattc ttaactgatt tctgtgagga
     2461 ctttatcgtg tcagacacac tgcccgggac aacgcatccc gacgacaatc acgaccgcct
     2521 ctggtgggga ctgccctgca ccgtgacgcc ctgtttcggg gcacgtctgg tgcagaaggg
     2581 taaccggttg cattaccttg ccgaccgcgc cggtatcaga ggccggttca gcgatgcaga
     2641 tgcataccac ctggaccagg cctttccgct gctgatgaaa caactggaac tcatgctcac
     2701 cagcggtgaa ctgaatcccc gccatcagca taccgtgaca ctgtatgcga aggggctgac
     2761 ctgtgaagcc gacaccctcg gctcctgtgg ttacgtttat ctggctgttt atccgacgcc
     2821 cgaaacgaaa acgtaaacca ccggaataac cctctgctcc agcctagtgt tttcaccacg
     2881 ccactgtccc gttttttatc tctacatatc aggaaaatct tcagtatgaa cacattaccc
     2941 gacacacacg tacgggaggc atcgcgctgc ccgtctcccg tcaccatctg gcagacactg
     3001 ctcacccgac tgctggacca gcactacggc ctcacgctga atgacacacc gttcgccgat
     3061 gaacgtgtga ttgagcagca tattgaggca ggcatttcac tgtgtgatgc ggtgaacttt
     3121 ctcgtggaaa aatacgcgct ggtacgaact gaccagccag gattcagcgc ctgtacccgc
     3181 tctcagttaa taaacagcat cgatatcctc cgggctcgcc gggcaacagg cctgatgacc
     3241 cgcgacaatt acagaatggt aaataacatt acccagggta agcatccgga ggcgcaacaa
     3301 tgaaactggc cctgacactg gaagccgaca gcgttaacgt gcaggcgctg aacatggggc
     3361 gaattgtcgt tgacgtcgat ggtgttaatc tcactgaact gattaacaag gtcgctgaaa
     3421 acggttattc actccgtgtg gtggacgact ccgaccaaca gtcaacctgc acaccaccac
     3481 cgttaacaac ccttaccggc atacgctgca gcaccgcaca tatcacggaa acagacaacg
     3541 cctggttgta ctcgctgtca caccagacca gtgacttcgg cgaatcagaa tggattcatt
     3601 ttacaggtag cggatatctg ttacgtaccg atgcgtggtc atacccggtt ctgcggctta
     3661 aacgcctggg gctgtcaaaa atgttccgcc gtctggttgt cacacttatc cggcgttatg
     3721 gcgtcagtct cattcatctg gatgccagcg ctgaatgcct gccgggttta cctactttca
     3781 actggtaacc agaaacaaca tgaaatcatt aatcactgaa acagcgctgg atatcctcat
     3841 tgcgtggctg caggacaata tcgactgcga atcgggaatt atctttgaca acgatgagga
     3901 taaaacggat tcggcagcac tgttgccctg tatcgaacag gcccgggagg atgtccgtac
     3961 tcttcgtcat ctgcagcttc tgcaccagaa ccggtaaata tcactcacca tacttcattc
     4021 cactgacgtc agcctgaaca gggctggcgt tttcgtttat aaaccaaaag ggaaaccgat
     4081 aatgtcagac atcacaatct cccgtccgga agtggtcacc gggcatacgg acgttatctg
     4141 ctcaaccagt atccgccaca ttctggctgt acgaaagagt acactgctgc aaatcgacac
     4201 acttatccgg caactggctg aaatatcagc aatgacagaa agtattggcg gtaaagccgc
     4261 cccggactgg gccatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga
     4321 aaccgcaatg aaagccatca cacgcaatct cgatcgcgaa atctggcgtg acctgatgca
     4381 acgttcaggg atgctttcct taatggatgc acaggcccgt gatacatggt accggtcact
     4441 ggagtacgat aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact
     4501 acaccagaac aaggatgagg tgtttgagcg aggggtgatc aacgtcttca ggggactgag
     4561 ctggaattac aaaaccaatt gcccctgcaa atttggcagt aaaattatcg tcaacaacct
     4621 ggtgaggtgg gaccggtggg ggtttcatct tatcaccggg caacagactg accgactcgt
     4681 cgacctggaa agaatgctac atctgttcag cggcaaaccg atccccgaca accgggaaaa
     4741 catcactatt cgtctggatg gtcacatcca gtccgttcag ggtaaagagc gctatgaaga
     4801 cgagatgttc atcattaaat actttaagaa gggatctgca cacatcactt tcaaaaggct
     4861 ggaactgatt gacagaatta acgatataat agccaggtac tttccttctg tgctctcagc
     4921 ctgatcccga gtgtgattcc ctttcgatat caaaagggac tgcgggtata attgagggta
     4981 catttttaat caaaccaaac aaattgcatt aatatcaata tgataaaaga aattttcgat
     5041 tccgagtccg gcaccactat tctaaagaac ccgcccacaa ggcgggtttt tgcttttgga
     5101 tttgacaata accttcacga aaaaaattag cttataaagt ctgggggaat tactctcgcc
     5161 acgttaacga gagtaatttt attgatatta atctcctgat actttacccc cgtccaaact
     5221 ccagtcgctg cacattcacc atcccaggct tctcacctgc gctgacatca atttgtatca
     5281 cccgcagcgc atatttttca tccagtgcct ttaaccagtt cagcaggtca ttaaacacca
     5341 caggttctat ccagacttga atattctccc cacgctcggc aatccgtttg atgaccaccg
     5401 agtgcgcgga agcgctgtcg ctgatgaccc gcgatacctg tgctggcgtt gtcgtgccgg
     5461 attttcgcgc cgcaacaata tccggcgcag tgctcttcag tcgcgcgttc atcgccacga
     5521 gctgctgcaa catcgtctcc tgttgttcta tccgttcgct caacggctgc cagatgagaa
     5581 cgtaatatcc ggcgctaaac aggaacacta ccgctgccag taacatgcct ttttcacgcg
     5641 gagaacgccc cgccaggtgt tgtgtcagcc agtgttcgcc acgacttaac tggcgttcac
     5701 gccattgctg aaaatagtga ataaatttat cgcgtaacat gttatttcct ccgcaacgtt
     5761 acgccgccgg aaaccgcatc accctctttc tgtaacgcgt cctgttgcac aacataatct
     5821 gccgccagtg cgctacgcag tttgtcgaag ctggcaaagt tcgcagcccg tagctggagg
     5881 tgaagcgtct ggcgtttttg atcaaaggtg aaaccacgca tttcgatgtc ggtaagtgac
     5941 gctgatttca gggtactggc gatcgctgac aattctgcga gcagccgggt atcgtcggcc
     6001 tgtgggcgat attttttcag cgccatcgtc acctgcgagc gtaaattcac aatccgcttc
     6061 tgctccggga atagcgttaa gaactgtttc tccgcctggg tgcggctttg cgccacctgt
     6121 tcgctgacgc tccataacgt cacgccccgt tccactacca gcgcaaccag aatcagcaat
     6181 atcggcagaa tcatcacccg ccagcgcgcc cactgttttc ggtagctgac acgaggctgc
     6241 cacggccctg ttagcaggtt cccttccggt tcgccataag tggtaatggc gggcagagcc
     6301 gtaacggtca ggcgttcggc gtccgcacca gcccatgcag acagttcttc cggtgcaatg
     6361 ccgaccacag ttagtgaaat cggtaaattc tgctcattga gctgtgcgcg gaacatgacc
     6421 ggagccagcg cccgcccggc gctccatccc cggcattcat cgatgcggca gataacccgt
     6481 tgcgcatcgc cagccataaa cccacaagga atggacatcc agtccggcgc gacggtagcg
     6541 cgggtgatgc cgtttgcctg caaccactgc gcaatgttgc gcatatgctg ctggtgaatc
     6601 acagctacgg ttgccagttg ctggtcgatt ttcaacgggg cgaaatgcag ttcatcgata
     6661 tcctggttca gctcttcttc cagcaaggcg ggcagaatcg tcggtatctg cttgcggggc
     6721 acatcaggca gttcaacctg ccagacgctg atccattcgc cgggaatgta gagtcgaatc
     6781 gcatcagttt gcagccattg ctggagacgt tcgtcagcaa cgtcaggcca gataccgtgc
     6841 tccacgtcgg gtgtatagcg ctgccaacgg atgggatcag cggcgcaaag cgggaaaaaa
     6901 atctcaagca tggaactcac tcactttctc ctgtctgatg ccagagaaca gaaaagtgtt
     6961 gtgggcccat gcggacaatt aacgaattca tcgtcagttc aatctcattc acggtgatat
     7021 ctgaacgcag ccagaagtaa ttgctgtcca cgctcaggac ggtttttagc tgttttttag
     7081 tacgctcatc gacgtctgca agtagcggct gagcaagaaa ctgatcgaca tcttcccagc
     7141 ccttcgccgg acgttgttgt aataatgccc gcgcctgaac agggcttaac cacgggtcaa
     7201 acagcgcctc aagaatcaca ctttgcgtga catctaatgt attgatgttg atttgctggc
     7261 gggccatcgg cagcgcacag accaacggtt tcagtttttg ataaagcccg gcgtccattc
     7321 cctgcaccac gcgcatctcg ctgatatcag ccagcggttg attagcggcg tagaacggca
     7381 ccgagcgggc gagatactcg ctgtcttcac gacccagacg cgtctgcacg ctgcggtctt
     7441 cgtcaataaa ctcccacagg ctttcggcta tcagttcggc ccgataagca ggcacatcga
     7501 ggcgcgagat cagggcaatc agttgttgta ccgcgagcgg acgcgacgcc gtcgtcggct
     7561 gagcgagggc attcaggtta aagcaggcct gtgcgtcacg cagagtgacg gcaatttgcc
     7621 cctgcggcag tgggaaaaaa cgcgggccag aagcccacgg ttgtgccaga tgcacacgct
     7681 tttcattttt caggctcaga ctgagcgcgc tcagcgccag gctttctgca ctggcgctgt
     7741 accacagcgc ctgctggtac tcctgctggc tgcgcgttcg cccaagttgt tgctgcatcc
     7801 gcccggaaag cgtgatggtt accagcatta tgaccgccag caataccagc accacgacca
     7861 gtgccattcc gcgttttggt ggtgaggtga tcatgataat tgcggcccgc gtaacaacca
     7921 gatgcgttca atttcgcccc attgtggcga atgcagggtt atgcgtactg ccacaggaat
     7981 cgcctgcact gatgaccagc tctcctgcca gcgcgtgccg tcgtagaact gcaaacggag
     8041 cgaatccgcc gggattaatt tttgcgttgt tggcttcacg ctgcctgccg catcggtcag
     8101 tggccaggct aaccgttcga gataaccacc atgaatgcgg taaccgacgg tgagcagatt
     8161 actgcgcggc agacgcatca acggattaac cacgccgcca cgtacaaaac gcatcccttc
     8221 actctcagac gccagcacgc cagcgcccgc cagtaacgct ggttcacgct ggccctgatc
     8281 gcctcttacc ggacgcggca tcatttgggt cagatcgtgg ttcaaaaaac tcatcgtttg
     8341 ctgcatgagg tttagttttt gatcgtgtcc ggcgacggcg ctattcacgc gtgtaacgcc
     8401 gtttgtcacc tgctgcgcca tcagtgccag tgaggcaaaa atggctatcg ccaccagcat
     8461 ttccagtaac gtgaaaccag cgcgagccct tctcactgtt ggcctcccac ggcgctaaac
     8521 caggcgcgtc gtgactgaat cactggcgaa aagtcgtcat gaagactgac ttcaatatcc
     8581 accgcctgga gcagcacgtt accggtattc agcggtgtgg ttcgccagta ccagcggctt
     8641 cctgccatat cactctcgcc ctggtgcctg accgctcgtt gttcacgggt catcaggtaa
     8701 agctgcgcgg tctgattttc agctacccaa cccgctaccg ttttttcctc aagaacgtgc
     8761 tgattactca gcgcgccgct ggcgatctgt aacaccgcca tggcggccaa cgcaaaaatc
     8821 gccagcgcga gcattacttc cagcaaggta aatccacgct tcatggcatc ctctcatcac
     8881 attgattgag agataacgcg ccatcgtgcg ccagctttac cgcccaacac gcgttttgcg
     8941 ccgccgagta gaagcgcagc gtaaacggcg tggcgggttc aaaaggcgaa aaacggatct
     9001 gcggcgtctt ctttttcgcc tctttttgca gctccagttc aatatcgtgc agcgtcaggc
     9061 gacgacgctg cagctccagc gcatactcct tctgccagat atcactgccg ggttgtaaca
     9121 gcatctgcac ctgtttcggc accgtaacct gtgccgataa ccgggtcgca gaaacgggca
     9181 accattgccc gtgacgacgc tgcataaact ggtaacctgg cgggtcgatt agcacaccga
     9241 gtgtttgccc ttcgatcact gccctgtcct taaactgcgc aaagcgagtc agaaaatcct
     9301 gcgccgcttt tttcgcaggc ggctctgaag cggtcgcaaa cgtctgtacc acgcccgcac
     9361 tggcaaggcc gataaggaaa atcaccagca tgatttccag aagtgtgaat ccgcgttcag
     9421 gcactgatta ctgaaactcc tgcaaattcc agttaccgat atctgcgcca gcgccctccc
     9481 cgttttcctg accatctgct cccagggtat aaacatcaaa cagccctttt tcacccgggc
     9541 tgagatactg ataatcattg ccccacggat cttttggcag tcgtttgatg tatccaccgg
     9601 tacggtagtt gcgggcgtcc gccatattgg ctggctgctg gatcagcgcc tccagtccct
     9661 gttcggtggt cggataacgc ccgttatcca gtcggtacat atccagtgcg ttttccagcg
     9721 ccacgatatc gctgatggct ttttgccgat cggctttctc tttgttgccc agcaggttag
     9781 gcactaccag acttgccagg acgccaagaa taacaatcac caccatcact tccagcaggg
     9841 taaaacctgc ccgtggtttt tgtgtgcggg ataacgaatt catgtgataa ctccgtaaat
     9901 tacattccaa ccatattgtt cagttgcagc atcggttcga ggatggcgat gacgataaac
     9961 agcaccacac ccgccatcat caccaccagc gccggctcaa acagccctaa cgccagcccc
    10021 acctgggtat caaattcccg ttcctggttg accgcggcct gttcaagcat ggtttccagc
    10081 tcgccgctct gttcgccgga ggcgatcatg tacagcatca tcggtggaaa caggcgcaga
    10141 tccgccagcg cggcgcgcag gctgcttcct tcgcggacgc gatctgccgc cagcagcagt
    10201 tgttgctcga cataacgatt tgccgacacg gcggcggcgg tctgaatgcc ttccaacagc
    10261 ggaacactgc tggcggtgag gatgcttaac gtgcgggaaa aacgcgccgt attcagtccg
    10321 cgcgcaacac gccccgtcac gggcaggcgc agcaaggttt tatcccagcg caggcgcatc
    10381 gcaggatttt tgagtaaccg ttgccccagc accagaagcc ccagcaaacc cgccagccag
    10441 tacacgccgc tggcctgtaa cgcgtcgctc atagcgatga gcattcgggt ggaggcgggt
    10501 agcgcgtgtc cgagatgatc aaactgttcg ataattttcg gcaccactgc cgtcagcaaa
    10561 atagtgacta cgcccgttgc caccaccagc agaaccagcg gatagagcat ggcctgcagc
    10621 aggcgtgatt tcagacgctg ccgctgttcg gtgtaatccg ctaggcgatt gagcaccacg
    10681 tcgagatgtc cggatttttc tccggcagcg accatcgaac aaaacaggga gtcaaagacg
    10741 cggggatgtt cgcgcaggct gtccgacagg gtataacctt cctgaatccg gctgcgcagc
    10801 gccattccga ggctttttac atgcagtttt tcgctttgct cactgaccgc ctgtaagcag
    10861 gtttccagcg gcattgctgc ctgcaccagc gttgccagtt gacgagtgaa cagcgccaga
    10921 tctgccgccg ccacgcgacg atgtgcgtgc cgccgacgct gcaacattcc ccctgccgat
    10981 gcattcatcc gggcttcaat gtgcacggga atgaggtctt taccgcgtaa caattgacgg
    11041 gcatgacgcg cggaatccgc ctcaatcatg cctttggttt tgcgaccatt acgctccagc
    11101 gcctgatagt aaaacagtgc cattacgcct ccatggttac ccgcagaact tcatcgagag
    11161 aggtttctcc ggcgagcact ttctcaatgc cgttactgcg gatacccgca gagtgttgcc
    11221 ggacataacg ttccagctcc agttccccgg cctgacggtg gatcaaatca cgcaatgtgg
    11281 cgtccaccac gatcagttca tggatggcag tacgtccgcg aaaacctttg tgattacagg
    11341 cggggcagcc ctgcggatgg tacagcgtga cggcatgcgc atcggtaatc cccagcaggc
    11401 gtttttcttc gtcagtggca ggcgcggcct gacggcagtc ggagcacagc gtgcgaacca
    11461 gtcgctgcgc catcacgccc gtcaaactgg aagagagcag gaaaggctcc acgcccatat
    11521 cctgcaaacg cgtgatcgcc cccaccgctg tgttggtatg cagggtggaa agtaccaggt
    11581 gtccggtcag tgaagcctga acagcgattt ctgcggtttc ggtatcgcgg atttcaccga
    11641 ccatcaccac atccgggtcc tgacgcaaaa tcgcgcgcag gccacgggcg aaggtcatgc
    11701 cgacgcgggt gttaacctgc gtctgaccga tcccttcaat catgtattcg atagggtctt
    11761 caaccgtgag aatgttacgc gagtggttgt tcagctcctg caatccagcg tacagcgtgg
    11821 tgcttttgcc ggaaccggtc ggccccgtca ccagaaaaat gccgtgcggt ttgtgtaaca
    11881 gctgacgcaa ctgcgcggtc agttcgtgac tcaaacccag acgctccagc gtcaggcgag
    11941 cctggttttt gtccagcagt cgcagcacca cccgttcccc ccaggcggaa ggcatggttg
    12001 atacacggac gtcaatcgcc cgaccgccca gcagcagcgc aatacggcca tcctgcggta
    12061 cgcgcttttc ggcgatatcc agtcgcgcca tcaccttgat acgcgagacc agcagcgagg
    12121 ccagtttacg accggggcgc aacatttcat gtaatgtgcc gtcaacacga aaacggatca
    12181 ccagactctt ttcaaacgtc tcaatgtgga tatccgaagc gccttctttg attgcctctg
    12241 ccagcatggc gttgatcagt ttgatgatcg gcgcatcgtc gtcactttcc agcagatctt
    12301 ccgtttccgg cagttcttca gcgagggtaa aaaagtcctc ggcggaaccg agatcttcca
    12361 tcagctggcg agcttcggaa gagtcccgct gccagaccgc attcagccgc tgttcaaatt
    12421 cggcctcgtc gatttgccgc agcgtaaagg gcgcgttcag cccccgttgt agctcctgca
    12481 aaacagagag cgacaacgga tgaacatgga ggatctccag cgacgcttcg caccatgcca
    12541 ccaggctaaa ccgacggctg aaactgtagg gcagacgcac ggtgttagcg gtggtttcct
    12601 gtgctacagg caccattaac gcgttctccc ggcattgagg aacgcgcgta cttccggcgg
    12661 taaggcctga ttttgcgctg gcaatatcgg ctgcgcggtg tgcggcatca ggcttaagcc
    12721 ttgctcatcg cggtagattt gctcagcgcg catgtagtta tatttgcgct gcgacacgcc
    12781 gtctgccgcc ataccgtcac gcaaaatggt cgggcggata aacaccatca ggttacgttt
    12841 ttctttttta tccgccgtcg atttaaacag gttaccaatc aatgggatat cgcccagcag
    12901 cggcactttc gccacgcttt ctcccgcctg gtcgtccatc agaccgccaa gcacgatcag
    12961 ctcaccatcg ttagccagca cggtggtttt cagtttgcgc tcgccaaaca cgacatcgag
    13021 actggtctgc ccttccactt tcgagacttc ctgctcaatc accatctgta ccgcgttacc
    13081 ttcgttaatc tgcggcgtga ctttcagcat gatgccgact tttttcctct ctaccgtatt
    13141 gaaaggattg ctgttattgg agccaacggt agatccagtt aataccggaa cgtcctggcc
    13201 caccatgaag aaggcttcct ggttgtccag cgtggtgata ctcggcgtgg agagcacgtt
    13261 cgagctggag tcgtttttga ccgcctgtac cagcgccatc cagtcgcctt tcaccacgcc
    13321 aaccgccgta ccgctaaagc cagaaagaag ctgagcgagc gtggagagat cgccgttagt
    13381 atccggattt atggtggtag caccgttttc gctgatcacc gtggaacctt tctgcggctt
    13441 ggctgcagaa atcgctgccc ccagcgtacc aatagggatt tgcgtaccgt tggcaaactg
    13501 cattaatccg gcatcttttg acccccactg cacgccgaag ttgatattgc tgccttcggc
    13561 aacttccacg attaacgcct cgacatgcac ctgagcacgg cgaatatcca gttgttcaat
    13621 cacgctttgc agcgactgca taatgtcctg cggcgcagta acaatcaggg cattactgtg
    13681 tttgctggcg gcgatggaga caacctcacg cccgctacca accgtgcctt ccgcctcttc
    13741 tttagccgcc gtgagcgtac cgctgacctg cttcagcaca tcgaccagat cttcggcttt
    13801 gctgtatttg agatagaaca cctggctgtt gccgctgcgc tccatttctg aatccagccg
    13861 acggatcaga cggcgcattt tgtctcgcgt ggcggggtca ccactgacaa tcacgctgtt
    13921 ggtgcgttca tcggcgacaa tttgagattt cagcgttgct ggctggttct cgccgctgtt
    13981 tttggtcagg ctttccagca cgcgggcaat ttccgaggct gaggcgttat ccagcgggat
    14041 cacctcttcg gtgcgattac ccgcgtgatc cacgcgctgg atcacttccg tcaggcgctc
    14101 cacaacggag gcgcgtccgg taagcataat cacgttggag ggatcgtaat taacaacgtt
    14161 gcctgagcct gcgctgtcaa tcatctggcg cagaatcggt gccagctcgc gtaccgacac
    14221 attgcgtacc ggcacgactt tggtgaccat ttcatcgccc gcgtagttgt cgctgccttc
    14281 accgaccagc ggcagcggct cgactttcgc ggcgcttgat ttcaccacct tcagcacgtc
    14341 gttttccatc ggtacgaccg catacccctg tgcttccagc aggttaagga ataactggta
    14401 atactggcgt tcattgagcg gagtcatagt gcgaatactc actttcccct gaacgcccgg
    14461 ccccataatg atggttttat taaggttagc gccgacggtt tcgatgaacg atttcaggtc
    14521 ggtatcttta aaattagcgg tgaaagtggc ttcttccgcc cagaccggtg aactacacaa
    14581 tgccgctgcc agcatcagcg gcagtaaacg cttttttgtt ttgaggccag ttgtcttctt
    14641 acgccagata gacaacgtca tatcacgcca aaacacgatg aatgattctc ctggattaaa
    14701 tgcggttagc gcagtgcgat ggaaatgtcg tagcgcgcac ccttgcgtaa aaccgtaagt
    14761 tgaatggaat ccattgaagg taactgccgc atcagagcaa tcattgctcg tggatcagtg
    14821 aaatcctgct gatttagcgc aatggcgata tcgccttccc tgaaaccgct ggcatcgaac
    14881 agagaacgat ctgcccccgg cttcactgca taaccgacaa ttccctcctt acgcacaggc
    14941 gtaagctgga tatagttaaa aattttctgc ggatctttcg ccagtgcctg acgcacggca
    15001 gccgggatct ccactggcgc actgacgaca ggttcagcaa cagcttgctt tgcttcgtca
    15061 ctggcggctt ttttgctggt caccgccaca ggggggcgtt cctcctctgc cagactcaga
    15121 cgttccattt tcccctgata gcgcagcatc acatggtcgc ggttgatttc ctcaatcact
    15181 gcgttgtgag agccaagccg ttcaccctgc aaatagacct gctgtttgcc gccttcttca
    15241 atcaccgcgc cgggtctggc actaaaggcg atcccacgca gcaccacatt aagacgcgtt
    15301 tctgccacag gcgcagattc aggttgtttt accggtgcgg cgacgggttg atatttgcca
    15361 aaccagtttt gttggctgac taattgcacg tcatttttat cgaacggttt tgcatccgca
    15421 cggagcggtt tattcgccga tgcggaaacc gccgtatatt ccgcagaaaa ggagaaatag
    15481 cgccagagtg aatacgccat ttttgcagaa ataataagca gcatgagcca gaacatcccg
    15541 cgcaccattt ttcgcagatg ctctttgtct gtattaagag attgtctctg gctaagaatg
    15601 tgacgaattt tagtcagcca ttgaatgaga taaattcgtg cgtcacgaaa aacaacccgc
    15661 gccaatttat atctacccga cgttatgctt tgactattcc acaggtggta cgatccagtt
    15721 tccgctgata ctggttacct gtacgtgtat cattaatcgt tatgctgtaa ttaattccct
    15781 cggtgatcat taatttcacc gttgggtcag cgcacatttg tcgttgatag ctggttaaaa
    15841 aggcgtcagg tgtctgcgtg gtctgagtac ccgattcgct gataatggtc atttttaccg
    15901 tcgagccact actttgcgcc agcactaagg tatatcccgc agatttaatc ggcaggtttt
    15961 ggctgatatt ttgcgcctgt tttttcgcca gtaaactggc gttttcatta tggctggcgc
    16021 agccacttaa taatcctgta acgctcaaca atagcgatat taataccctc cctggcatct
    16081 gttttatcga catggtaaat aatctctaag gttattaata agagttaaaa tgtcactttg
    16141 ataatgacgt ggttatcatt aaaacaatgc ctgtagataa agtgttgcta taccgcccag
    16201 acttaaacac ggtccaaaag gcagtgtggt tgatcctctt tttgtaataa cggcatatat
    16261 cagaccgcag catgatgcga ttaaagccac attgggtagc gacaacggcc ccacccagtc
    16321 acctaaagcg gcgaagagta atacatcgcc catgcctaat gcttctttac gcagaactat
    16381 cccggctatc cagcgcaggg agtaaaaagt gataaacccc accagaacgc cggtgactgc
    16441 gtcttgtagc gttaacggac tctgctgcgc ccatgccgca atcagtcctg tccacaatac
    16501 gccctgagta aaaacatcgg gcagccattg gttgtcgagg tcgatgatgc tcgcggcaat
    16561 cagccacgcg gataatatca tcaccgccag tccccatcca ctttctggcc acaccagact
    16621 cgccagcaaa aaagcgagtg ctgtcaataa ttccaccagc ggataacgct tgctgatttt
    16681 cgcctgacag tcacggcagc gccctttgag catcagccag gagagcagcg gaatattgtc
    16741 acgaacgcgg atagtctgct gacaatgcgg acagtgcgaa cgcggtagcg caaggcttat
    16801 ttttgactgc gcactcggca tttcaccatg aaactccgcc atttgttggc gcagcatgat
    16861 ggggtaacgc caaatcacca cattcaaaaa actgccgatg atcaatcctc cgacggttgc
    16921 cagtatgggc atcgccgcgg ggtattgctg aaaaacatca aaaagcatgg ttaaaggttg
    16981 tttgttgtaa tttgccggat gcggcgtgaa cgccttatcc ggcctacagg tgtctgccaa
    17041 cgcaagtaat cgcggcttct tccctccagg cccgataagc tggcgcatcg ggcatatatg
    17101 ttgacccggt gcgccttatt tcatgccgga tgcggcgtga acgccttatc cggcctacag
    17161 gcttattcgg ctggcatcga atactcggta accttattaa tggtttccgg ccctttttcc
    17221 ggtttcgaca ggtgcatcgc cgcgagcgtg ttgtaagccg actggctcac tccgccttcg
    17281 aagctcatct cacttgcccc cggaagctgg taagcatttg cgcccggatt ccatttctta
    17341 aagaactccg aaagatccgt ctgagcgacc caggaggcac acagcatcag cgtgtcggca
    17401 gcgttaccat tggattcagc acagtaattc ttgccaccaa aggtgctgtt accaacatca
    17461 tcgccgcgtg ctttacgatg catcaactgg aacaggttcc agcctttcat cccttcacgc
    17521 tcgctgtaga acgctggcag cttgccatct ggataccact tcgtgatatc aaagtttttc
    17581 tctgcccact ctttcagctg tgcgtacatc agcagacggt cccccgcacc gccgcgcgcc
    17641 caggcctgac cgttgctctc gtccagatat tccggcgcga cggtaatgtc gtcagcgaca
    17701 cggttcatct tgccgagata gcgatcctgc atgtacagcg ccagcacgtt gttcgccact
    17761 tcagttgcac ccggtacgtt cagcggtgtt tctgcagcgt tatgaccgac ttcgtgccaa
    17821 atcagccagt cgttcagcgg cgttgtcggc agcgtggtgc tgttcgtcga gaagctgctg
    17881 ttcattaccg gataacccga gtgcgcatca ccgatggaga tctgcacatc gttggcgaaa
    17941 cggtgtttat gccccgtcaa tgcttcatag gtaaacatcc ggtggttacc gctcgtttca
    18001 ttgcgaccgt agaagtcatt catcgagctg gcaaaggtat ccagatcttt agcgaattct
    18061 gctacaccac cagtgaaatt gctggcctca aggttcttct tcggcgtggt atagacgaac
    18121 gacgcagact ccagctcgcc cagcggcgcc ggtgagtcca gatcgttttt ccattcgccg
    18181 tctttataga acggcgcttt tactacaccg gtaaaggtga agttagcaga aacatcatcc
    18241 ttactgtcgc ccttgatata aatcagacca ccataaggca ccttgaaggt cacttcaccg
    18301 ttagcctcca gagtatacgt tttagtcact cttggcggac ggttcagcgc aacttcatgc
    18361 ttctcacgtc cagtcaggtc gtcagccagc gccacggtaa cagtcactgg gactgacgcc
    18421 gaagacttaa tggtgacgtc ctgctgggcc ggtgcccaca ggccggttga ctgcatgtta
    18481 cccgcaaacc atttggtcgg attcgagtac aggctgatgt tttccgtaac gctctcaccc
    18541 tttgccgata cggatcctgg gtacttctcc acatcaacct taatgttcag atcccaccag
    18601 gaacggccca gcatcagacg cgtcagcggt ttttccatat agttgagcgg atagcttggg
    18661 ttcatcatgc ccgctttgct gctaccgtca ccgtagatca tgttgttatc gaccagcgat
    18721 tttttcagat ctgcggagca cttggtgccg cctgcatagg catcattggc gtagcagttc
    18781 aggaactcgg tgaacgtttt aaagcccagc tcatcttcct tgccctcttc gtaacgatat
    18841 ttcgtatcgt tccacagcca gaccgacatg ttctggtaca gacgttccag atcaacactg
    18901 ttcagacgct cacctttact gcctttggta cggaaataaa gctcatgctg atacaggcgc
    18961 tgaatgttgg tgcctaaatc cgccgcctgc accatcgctt tcgcggtgtc ggcgtcaaga
    19021 ttcagttgcg tatagcgcgg aacatacatg ccacctgtta ccggaacatc cgtgcctggg
    19081 cggcgctcca aacagttaat ctcgtaatgg taagtcgagt ccttacactc ctgtaaccca
    19141 ggaaacttct caaagatttt tgcctttgcc gcttccagag attcttctgt tgtgtattcc
    19201 gcttcatcaa taaaggcata acgcgttacc tgtttgccct caacttcctc ctgccagctc
    19261 gcaacttcca gtttcggctt gtcatcaggc ttgttgtctt gctggtattt ccaggtcact
    19321 tcccctgtat ttgggtcgat ggtgtacggc ggttgcgcgc cgtctgcagc aggataacgt
    19381 tcataaaccc aaatgccagt cgcgcgacgc tgacgaacgc gatccggata cccttgcgga
    19441 tcgttgttca ccaccgattt gttcagagcc attgacagac ccgcggcatc cagcagacgc
    19501 acaaaactgg acgcgctctc ttccttaaga ttgctcatca cgttttccat gatcagcacc
    19561 gagccacctt tgttcagata agcgatcaga tcggtcacat cctgctgagt cagcttcggt
    19621 ttgctggtat ctgcacgcag aggcacagca tagggatcgc cagaccactg agtcacatat
    19681 tcaaagccgt tgaggatcag caacggaatc tcttccggat tcagatcgcc atagctggtc
    19741 aactgtttaa ccgtgatacc agtgaaatcc tcatggaaag caaatggtgc actatttccc
    19801 aatacctggc ccgctttttt gaaataaacg ttctccaggt tggtgccgac agtcatgatg
    19861 ctcttggtat ttggctgcca gatgtcattt gacaagtagc gcagtacgtt ctgcataaag
    19921 tgcttcatgt catcagaatc accgctgagc gtacactcac ctttactatt aacaccaccg
    19981 ccccaactgt aaccattagg gcaacgcaaa atgctgttgt agtgcgggtt accgataacc
    20041 atcagtttgc cgtcgccgac ttgccccagc gaaataaacg gcaggttgaa ggtcgcggta
    20101 tcgcgcgtaa cgttctctgg ctgcacaatg gaaggcgctt ccgtaatgta cgccagctca
    20161 tttttatccc aggcgcgttt ttcaccgaag gccagccagt agtttttatc attacgcgcc
    20221 atcagaatcg ggaatgccac gttggagata ttcaccaccg cctgaccgcg cgcgttaccg
    20281 gtgctgccat agaagttggt agagtcatgg aagacgtgga acttgctgac agactgatag
    20341 ttcgtatcca cgccccacag cttgttgata acgccctgaa tctggccgtc attaacattg
    20401 cgcgttgtca gcgagaacca gcgagcttcg ttgcaaccgt tggttttcgc acaaatcgcg
    20461 gtatcgatct ctttggcctg acccgtctta aactgctcga taaattcgtt aggcagcaca
    20521 acgttttgat cgccttcatc cagcgtcgca ccgttggata acgaaagatt gattatctcg
    20581 ttgatcacgt tgggatattc ggcaaagacc ttgcgtacat cgtccggaac aacacgagta
    20641 ttattttgac cagtcgtcga ataacgatga atgagctgat cgatatttgc cccgcgaact
    20701 tcatcaccca attcagtcag cgcaatggtc gacttattgc cacgtactga gcccagttca
    20761 aaggtatcga taccaaagga gatggtttcg ccccagctaa aggagaattt accgttttcg
    20821 tcagttacgc cacggcctga attggtgtag tagtcaacgc cagcaacacc gttccccagg
    20881 ctatccacca gttggccttc ggaaagaatg atttcagtgg gttgatactg ataaaactgt
    20941 tccgcgttag ccgacacgaa ggacgcgttc agatccggtt ttgttccctc tgtcgtgact
    21001 ggcactaccg ttgaggtatg ggtggacggc gctttatcag tcgcagcatt gttttccacc
    21061 tcttcattga ccagcttgct gaaattgtct gttgccagat caatttgctt atacagtttt
    21121 tcaaatcgcg cgcgatcaac cactgacgag aaagtaagac aaagctgttc tgcatctgcg
    21181 gggcaactgt cgctggacgt caccagagag atggcgttgg ttttcttatt ttcagaattc
    21241 gccagctcct gcgcgtcctc caggctaaac gacactttgt caaccgcacg caggctacgc
    21301 gcagcttctg actgggtgtt gaatgttgca atggtcgtac tgcccaccac acaactcacg
    21361 gtattgcctg gcgtaaaggt aaagccatcg ctggattcac cattacaggt agcaccagtt
    21421 acccgctggc ttccgcccag ggtcagataa cccgttttcg taggaacagg ttctggctcc
    21481 ggttctggtg tcggctcagg atcaggtgtt ggatccggcg taggttctgg gtccggcgtc
    21541 ggctcaggtg tcggctccgg ggttggtgtt ggatcgggtt tcacttccgg caaagtccct
    21601 gatccagaat ctacagacgg cgtatcggag gac
//
LOCUS       DADSZD010000073        21624 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.073, whole genome shotgun sequence.
ACCESSION   DADSZD010000073 DADSZD010000000
VERSION     DADSZD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.073"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            78..1133
                     /gene="phoE"
                     /locus_tag="J1Z28_002444"
     CDS             78..1133
                     /gene="phoE"
                     /locus_tag="J1Z28_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBA7717272.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTSEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1172..1573)
                     /locus_tag="J1Z28_002445"
     CDS             complement(1172..1573)
                     /locus_tag="J1Z28_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBA7717273.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(1631..2875)
                     /gene="frsA"
                     /locus_tag="J1Z28_002446"
     CDS             complement(1631..2875)
                     /gene="frsA"
                     /locus_tag="J1Z28_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBA7717274.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(2967..3425)
                     /gene="gpt"
                     /locus_tag="J1Z28_002447"
     CDS             complement(2967..3425)
                     /gene="gpt"
                     /locus_tag="J1Z28_002447"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA7717275.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            3686..5143
                     /gene="pepD"
                     /locus_tag="J1Z28_002448"
     CDS             3686..5143
                     /gene="pepD"
                     /locus_tag="J1Z28_002448"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBA7717276.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5200..5814)
                     /locus_tag="J1Z28_002449"
     CDS             complement(5200..5814)
                     /locus_tag="J1Z28_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBA7717277.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5811..6962)
                     /locus_tag="J1Z28_002450"
     CDS             complement(5811..6962)
                     /locus_tag="J1Z28_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBA7717278.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7140..7592)
                     /locus_tag="J1Z28_002451"
     CDS             complement(7140..7592)
                     /locus_tag="J1Z28_002451"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7717279.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(7602..8000)
                     /gene="yafO"
                     /locus_tag="J1Z28_002452"
     CDS             complement(7602..8000)
                     /gene="yafO"
                     /locus_tag="J1Z28_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBA7717280.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(8003..8296)
                     /gene="yafN"
                     /locus_tag="J1Z28_002453"
     CDS             complement(8003..8296)
                     /gene="yafN"
                     /locus_tag="J1Z28_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBA7717281.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(8348..9403)
                     /gene="dinB"
                     /locus_tag="J1Z28_002454"
     CDS             complement(8348..9403)
                     /gene="dinB"
                     /locus_tag="J1Z28_002454"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBA7717282.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(9474..10397)
                     /gene="lafU"
                     /locus_tag="J1Z28_002455"
     CDS             complement(9474..10397)
                     /gene="lafU"
                     /locus_tag="J1Z28_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBA7717283.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHLPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPENKVMQVSAMADQMLLDAKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            complement(10400..11263)
                     /gene="motA"
                     /locus_tag="J1Z28_002456"
     CDS             complement(10400..11263)
                     /gene="motA"
                     /locus_tag="J1Z28_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBA7717284.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAGMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            complement(11276..11992)
                     /locus_tag="J1Z28_002457"
     CDS             complement(11276..11992)
                     /locus_tag="J1Z28_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HBA7717285.1"
                     /translation="MLQDVFESEEFAAAPILTPQQESHYLQAYLPLVRKVVRHLAPQC
                     NCIIDRQDMEQTALIGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWPELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGIPLAGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            complement(12012..12479)
                     /locus_tag="J1Z28_002458"
     CDS             complement(12012..12479)
                     /locus_tag="J1Z28_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HBA7717286.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            complement(12487..13548)
                     /locus_tag="J1Z28_002459"
     CDS             complement(12487..13548)
                     /locus_tag="J1Z28_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HBA7717287.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQHQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTSLPPQLQELIGQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARVVLQVKTPDLVSEHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            complement(13545..13856)
                     /locus_tag="J1Z28_002460"
     CDS             complement(13545..13856)
                     /locus_tag="J1Z28_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBA7717288.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLMRQFHDYAAA
                     ETDAAQLDTVKAQLYGAFNTLIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            complement(13861..14253)
                     /gene="fliS"
                     /locus_tag="J1Z28_002461"
     CDS             complement(13861..14253)
                     /gene="fliS"
                     /locus_tag="J1Z28_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBA7717289.1"
                     /translation="MYQVQDGYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAR
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            complement(14276..15592)
                     /gene="fliD"
                     /locus_tag="J1Z28_002462"
     CDS             complement(14276..15592)
                     /gene="fliD"
                     /locus_tag="J1Z28_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBA7717290.1"
                     /translation="MINPRTIAQEIAYADVATQAANLQEKQSELDAESSGLDSLSSAL
                     SDFQSAVDALNSDTDGPVTFAATSNNDSATVSANSQAQAGSYSFFVEQLAQGQQTTFS
                     MGDDAFSATGTFELTMGDSTMDIDLSAADQNGDGDGFIDASELVNAINDSDDNPGVSA
                     ALVKTDGTTTIMLTSDSTGAQSAFSVSVTGHDASNDSASAPVATDVSSAQDAIIHLGS
                     ASGPAITNSSNTFDDVIPGVTMTFTEVSDSDSDLTTFNISEDSSASQEKVQTFVDAYN
                     TLIDTVDSLTTHGDDSSSAGVFAGDAGLSSLANQLDDIAHASYNGVSIVDYGITLDSH
                     GHLQIDSDQFSDEMAKNPDGLTSIFVGDNSMVAQMDDLINIYTDSSNGIITLRQQNID
                     DQMSKIQDEGDQLTDTYNANYDRYLEEYTNTLVEVYTMKASMAAFA"
     gene            complement(15799..16713)
                     /gene="lafA"
                     /locus_tag="J1Z28_002463"
     CDS             complement(15799..16713)
                     /gene="lafA"
                     /locus_tag="J1Z28_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lateral flagellin LafA"
                     /protein_id="HBA7717291.1"
                     /translation="MLSINTNNASMAAVNAISKSSSSLSTSMERLATGNRINSSADDA
                     AGKQIANRLTAQSSGMGVALSNINDATAMLQTADSMFDEMSDVLGRMKDLSTQAANGT
                     YSDDDLQAMQDEYDELGQQMSDMLQNTTYGGTNLFGVSGTSNTGTDGLFQSAVTFQVG
                     AESSDTMTVNISSQLNTLVTDLSAISNSFSADQADTTGTAGVSGGTELTASGSANQMI
                     TSISTAMDDVSQIQSKLGASINRLNDTANNLTSMQDNTEVAIGNIMDTDYATEASNMT
                     KQQVLMQTGITMLKQSNSMSSMVSSLLQ"
     gene            17200..18042
                     /locus_tag="J1Z28_002464"
     CDS             17200..18042
                     /locus_tag="J1Z28_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadC family transcriptional regulator"
                     /protein_id="HBA7717292.1"
                     /translation="MSIIINNWRMDPSLNALIHCETGETRRLGEYHFILLETLAKNAD
                     VVLSRSYLCAEVWKNRIVGGNSLPTAIHALRVAIDDDGKQQNIIKTIPKKGYLCNKEY
                     VSLPESSPAEALIITDQIQDTAPEEISSTTPPVPVRKKHKGLMGLALAAAVIFIGSTV
                     GYSHLKSTPDAPQLVKESINSPRIKIFHLTSAGENNSVPLLSQTLAPGKDKLDNLLSA
                     HNMTMTTYYKYVRNRLESDIVLRNQCNGSWQLTFNVEGWQNSDINSAMYQNLEKLLNT
                     VQKC"
     gene            complement(18101..19078)
                     /locus_tag="J1Z28_002465"
     CDS             complement(18101..19078)
                     /locus_tag="J1Z28_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein"
                     /protein_id="HBA7717293.1"
                     /translation="MQVGLTSSSLATGGAHSAAVSSSTVAPTQAVRQKLPATASEYPA
                     SPLITTRPQRYSVQLNDQLTTLQQADHYLGQLEQQLLDYRHSQRKGGQAQSTALMQML
                     DKRAALSGGAVDRQLQPVLQGEARVTFHSPDLANLVHNPTPGTRMFSVSDGRQTQLSA
                     VMLSEDDSAAQYQTRLTNALRRVGVQMHQHADGISFSTTEKQWPHIERTLSVRTDGDK
                     SAFMPLKTFAEPSQADRLAQSLQQGGAGISQMLENINQQRAQMAVQQEKARQLIDGMS
                     RFPQTESAVQASENLGGVLDSANHNYQVLLQAVNGQARISSQTVRNLLG"
     gene            complement(19095..20024)
                     /gene="flgL"
                     /locus_tag="J1Z28_002466"
     CDS             complement(19095..20024)
                     /gene="flgL"
                     /locus_tag="J1Z28_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein 3"
                     /protein_id="HBA7717294.1"
                     /translation="MRVTTQQTYVSMTQSFNNLSGDLAHVVEQMATGKEILQPSDDPI
                     AATRITQLNRQQSAIEQYQSNIDSASAGLSQQESILDGVNNSLLAVRDDLLEAANGTN
                     TADSLASLGQDIESLTESMVAALNYQDEDGHYVFGGTINDQPPIVAVDDDGDGVTDSY
                     SYQGNSDHRQTTVSNGVEVDTNVAASDFFGSNLDVLNTLNSLSQELQNPDVDPADPQV
                     QSDIQNAVDVVDTASDDLNASIASLGETQNTMSMLSDAQTDISTSNDELIGSLQDLDY
                     GPASITFTGLEVAMEATLKTYSKVSELNLFSVL"
     gene            complement(20039..21415)
                     /gene="flgK"
                     /locus_tag="J1Z28_002467"
     CDS             complement(20039..21415)
                     /gene="flgK"
                     /locus_tag="J1Z28_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBA7717295.1"
                     /translation="MDMINIGYSGASTAQVELNVTAQNTANAMTTGYTRQVAEISTIG
                     ASGGSPNSAGNGVQVDSIRRVSNQYQVNQVWYAASDYGYYSTQQGYLTQLEAVLSDDN
                     SSLSGGFDNFFAALNEATTSPDDSALREQVISEAGALSLRIDNTLDYIDSQSTEIISQ
                     QQAMVSQINTLTSGIASYNQQIAQAEANGDNASALYDARDQMVEELSGMMDVQVNIDD
                     QGNYNVTLKNGQPLVSGQQSSTIALETNADGTPTMSLTFAGTTSTMTTDTGGSLGALF
                     DYQNDVLTPLTDTINSMALQFADAVNNQLAQGYDLNGNPGEPLFIYDASNADGPLTVN
                     PDITADELAFSSSPDESGNSDNLQALINISTEPLEIANLGSVTVGQACSSIISNIGIY
                     SQQNQTEVDAASNVYSEAQNQQSSVSGVSMDEEAVNLITYQQIYEANLKVISAGAEIF
                     DSVLEMCS"
BASE COUNT         5009 a         5718 c         5464 g         5433 t
ORIGIN      
        1 tcaggttaaa aactttcctg ttttcaacgg ggctctcccg ctgaatattc gcgcgttaat
       61 taaaatcagg aatgaaaatg aaaaagagca ctctggcatt agtggtgatg ggcattgtgg
      121 catctgcatc cgtacaggcc gcagaaatat ataacaaaga cggtaataaa ctggatgtct
      181 atggcaaagt taaagccatg cattatatga gtgataacga cagtaaagat ggcgaccaga
      241 gttatatccg ttttggtttt aaaggcgaaa cacaaattaa cgatcaactg acgggttatg
      301 gtcgctggga agcagagttt gccggtaata aagcggagag tgatactgca cagcaaaaaa
      361 cgcgtctcgc ttttgccggg ttgaaatata aagatttggg ttctttcgat tatggtcgta
      421 acctgggcgc gttgtatgac gtggaagcct ggaccgatat gttcccggaa tttggtggcg
      481 actcctccgc acagaccgat aactttatga ccaaacgcgc cagcggtctg gcgacgtatc
      541 ggaacaccga cttcttcggc gttatcgatg gtctgaactt aaccctgcaa tatcaaggga
      601 aaaacgaaaa ccgcgacgtt aaaaagcaaa acggcgatgg cttcggcacg tcattgacat
      661 atgactttgg tggcagcgat ttcgccatca gtggagccta taccaactct gatcgcacca
      721 gcgagcagaa cctgcaaagc cgtggcacag gcaagcgtgc agaagcatgg gcaacaggtc
      781 tgaaatacga tgccaataat atttatctgg caactttcta ttctgaaaca cgtaaaatga
      841 cgccaatctc tggcggcttt gccaataaga cgcagaactt tgaagcggtc gctcaatacc
      901 agtttgactt tggtctgcgt ccatcgctgg gttatgtctt atcgaaaggg aaagatattg
      961 agggcatcgg tgatgaagat ctggtcaatt atgtcgatgt tggtgctacg tattatttca
     1021 acaaaaatat gtcagcgttt gttgattata aaatcaacca actggatagc gataacaaat
     1081 tgaatattaa taatgatgat attgtcgcgg ttggcatgac ctatcagttt taatggatat
     1141 tgccggatgt gatgcatccg gcacatttca ttcacgccgt taacttcacc ggttcatcac
     1201 gaaaatcatc tgccggttcc agcttcagat tcagcgtcgc cagcagctca cgcagcttct
     1261 cgtgaaattc acgcagggtg tgctccagtc gttcaaccac ttcggcatct tttatctgaa
     1321 cactcgtcca gtcgcctgct ttatcgaaca gaccaaactg gtaactgtag gtaaaacggg
     1381 attcctgcgc ttccagctcc atccaccagc cccagaactc acgcacttcc ggtgccggtt
     1441 tcacgttgac gcatacagcc agacaatcga aaaagaatcg attatcttcg cacttacctt
     1501 cacgaatata cgggcctagt gcggtaaatt ttttgactaa tctgctcttc gggtgtccac
     1561 tcggtaacgt cattgcaatc tcctgttgtg atgcaactgt tttaccaaat tggtaaaatt
     1621 tagcaaattt ttaacacaaa cgtttttcga tccagtctgt gatttcctga aggcctttgt
     1681 caaaattccg atacaccggg ttaaatggga tctctaataa tttaccgtca gcagatgacg
     1741 acgtgattaa gcgtgagtcc tcttccgggc tgaacggatc gttcttccag tagcctgata
     1801 acattggcgt tgggcagcga cgtcccagca atccttgcac ttttaatgaa tagcgattca
     1861 actccactcg caacgcttca tcggaagcat catgcatccc taaacgactg gccagaacgt
     1921 caagatacat ttccggcacc tgttgctggc acttaaaatc actcaacagg gtatgaacta
     1981 ccggaccaag acaggcaacc actttcagac gcggcgattc aaggtatgcc agacgcacgg
     2041 caacgttagc gccgaaacgg aaaccaaagg ccgcgacgcg agtgtgatcc acccacggta
     2101 cgttaggcag cgcctttaaa acgtgctgat gcaacaggct ggagtcctgg gtgagcttcc
     2161 attttgaaga aaagcccacc gacggcatat caatagtcag catagcaatg ccgcgcggcg
     2221 caaaataacg ttcatacagg ctgtaatagt ccgtctgcat cgcatccaga ccaccacaca
     2281 ttaatactgt cgggaacggg ccatcgcctt tcggcatatg caaaaagccg gtgatgggcg
     2341 caccgccggg tacggtaaac tccatctgtc gcatcgtgcc cggcagacgc tgagcggcct
     2401 cttcataggc gcggtttgac aaagcctgcg cttgctcggc caggtcatct cctttcagat
     2461 gaggataggc agcaatgttg tacaacgtag ccgcatgcag ccagtgtcga ccgctgagtt
     2521 gcggatcttc ttccgcacag gctttttgtt gccacaccat cgcctgggta gcccactcat
     2581 aaatccagtt gccgccgcgg tagccaatca ccgtatcgta taaatcatcg tcggtacgtt
     2641 cggcatcgct catcacaata cgcgcctgga cgtcgaggat ttcacggggg tcaatgccgc
     2701 gccagatcca catcagacgg ttaatcatgc gataccagtg agggatggtt ttaccatcaa
     2761 gggccgactg cacaggcggt tgtgcgccgt gactaaagcg gcggactagc gtcgaggttt
     2821 caggatgttt aaagcggggt ttgaacaggg tttcgctcag gtttgcctgt gtcatggatg
     2881 cagcctccag aatacttact ggaaactatt gtaacccgcc tgaagttaaa aagaacaacg
     2941 cccggcagtg ccaggcgttg aaaagattag cgaccggaga ttggcgggac gaatacgacg
     3001 cccatatccc acggctgttc gatccaggta ttttgcggga tatcaacaac ataatcatca
     3061 accagcggac gaccagccgg ttttgcgaag atggtgacaa agtgcgcttt tggatacatt
     3121 tcacgaatcg caaccgcagt accaccggta tctaccaggt catcaataac gatgaagcct
     3181 tcgccatcgc cttctgcgcg tttcagcact ttaagctcgc gctggttgtc gtgatcgtag
     3241 ctggaaatac aaacggtatc gacatgacga atacccagtt cacgcgccag taacgcaccc
     3301 ggtaccagac cgccacggct tacggcaata atgcctttcc attgttcaga aggcatcagt
     3361 cggcttgcga gtttacgtgc atggatctgc aacatgtccc aggtgacgat gtatttttcg
     3421 ctcatgtgaa gtgtcccagc ctgtttatct acggcttaaa aagtgttcga ggggaaaata
     3481 ggttgcgcga gattatagag atctggcgca ctaaaaacca gtatttcaca tgagtccgcg
     3541 tctttttacg cactgcctct ccctgacgcg ggataaagtg gtattctcaa acatatctcg
     3601 caagcctgtc ttgtgttgac aacattttct gctaaccctg tgacctgcaa tactgttttg
     3661 cgggtgatcg acaaggagac ttaacgtgtc tgaactgtct caattatctc cacagccgct
     3721 gtgggatatt tttgccaaaa tctgttctat tcctcacccg tcctatcatg aagagcaact
     3781 cgctgaatac attgttggtt gggcaaaaga gaaaggtttc catgtcgaac gcgatcaggt
     3841 aggtaatatc ctgattcgta aacctgctac cgcaggtatg gaaaatcgta aaccggtcgt
     3901 cttgcaggcc cacctcgata tggtgccgca aaaaaataac gacaccgtgc atgacttcac
     3961 taaagatcct atccagcctt atattgatgg cgaatgggtt aaagcacgcg gcaccacgct
     4021 gggtgcggat aacggcattg gtatggcctc tgcactggcg gttctggctg acgaaaacgt
     4081 ggttcacggc ccgctggaag tgctgctgac catgaccgaa gaagccggta tggacggtgc
     4141 ttttggctta cagagcaact ggttgcaggc tgatattctg attaacaccg actccgaaga
     4201 agaaggtgaa atctacatgg gttgtgcggg aggtatcgac ttcacctcca acctgcattt
     4261 agatcgtgaa gcggttccag ctggttttga aaccttcaag ttaaccttaa aaggtctgaa
     4321 aggcggtcac tccggcggtg aaatccacgt tggcctgggt aatgccaaca aactgttggt
     4381 gcgcttcctg gcgggtcatg cagaagaact ggacctgcgt cttattgatt tcaacggcgg
     4441 cacactgcgt aacgccatcc cgcgtgaagc ctttgcgacc attgctgtcg cagctgataa
     4501 agtcgacgcc ctgaaatctc tggtggatac ctatcaggag atcctgaaaa acgaactggc
     4561 agagaaagag aaaaatctgg cattgctgct ggactctgtt gcgaacgata aagctgccct
     4621 gattgcgaaa tctcgcgata cctttattcg tctgctgaac gccaccccga acggtgtgat
     4681 ccgcaactcc gacgtggcaa aaggtgtggt cgaaacctcc ctgaacgtcg gtgtggtgac
     4741 catgaccgac aataacgtag aaattcactg cctgatccgt tctctgatcg acagcggtaa
     4801 agactacgta gtgagcatgc tggattcgct gggtaaactg gcaggtgcga aaaccgaagc
     4861 aaaaggcgca tatcctggct ggcagccgga cgctaactct ccggtaatgc atctggtacg
     4921 tgaaacctat cagcgtctgt tcaacaaaac gccgaacatc cagattatcc acgcgggcct
     4981 cgaatgtggc ctgttcaaaa aaccgtatcc ggaaatggac atggtgtcta tcgggccaac
     5041 catcaccggt ccacactccc cggatgagca agttcatatc gaaagcgttg gccattactg
     5101 gacactgctg actgaactgc tgaaagaaat tccggcgaag taattatttg atttgctgcc
     5161 ggatggcgtt taatcgcctt ccggcagttt catccttcat tacccttcga taaaagccat
     5221 ccctgtaaac gttcgtcgcg ggttgccacg ttcaatctgg tgatggaaca ttcgccgctg
     5281 cgatttcagc gccgcgctat tttcctgttg ctgttgttcc agcttccagg caatcagtaa
     5341 tcgcgccagc cgtttgttag catgctgact gcgctctgac tgaaccttca cgctaatacc
     5401 ggatgccaga tgcgtggcgc gtaccgccga gtcggtttta ttgacatgtt gaccgcccgg
     5461 ccccgacgaa cgcagcgtct cataacggat tgcatccgat tgttcctgct catcagcggt
     5521 aaaacgccca atgcccagaa accagttttt gcgcccatga tgaggccgat acggactcgg
     5581 acaaatccac tgaatagtgc cgcaccagct ttcgcttaga gcccatgcgt tatcgccatc
     5641 cagagaaatc agcgccgaac gcagtgtgtc agagtagcga cccgtttctg tttccagcac
     5701 cgttaccgcg acgtcctgtc gggtagcttc tttaatcagc ctgtccagtg cttttctcac
     5761 tgcgagacaa cattcttccg gcccctgagc agaggagagt tgtagcaaga tcatcctttt
     5821 ttccctccac tgtttttgag cgtcagcacc gggcgcagtc gcgcgacagg aataattaac
     5881 ccagccagca ccagacattg caccacgctt tcagccgatt tataggcttg tggcgcttct
     5941 tcaaagatga gttgtttatc gcgacaaatt acccgactgc caagttcagt ccgcgagagt
     6001 tgcgtcgccg tgtatttcgc tgccagacgc cctttacact cggtgcgccc ccatttacgc
     6061 cccgccccat gcgccagcga gtggagcgtt ttttcgttcg caacaggttt aaccagccag
     6121 gagtaatcgc cgcgtgaacc gggaataatc accagaccgt tgtcatccgg cgtggcacct
     6181 ttacgatgca accagccctg ttgatcgccg atttggcacg cgctaacaaa gttatgcgcc
     6241 acatccagaa ccggacttcc ggtggcctta acttgttgca ttatgcgcaa agcgatcagc
     6301 tggcgattaa tacgcgcaaa cgccagcgca tcgtcatgtt ccgcaatata gcgtagcgcg
     6361 tcgtcactgc cttcaggcaa accatgatgc gaaaacgagg caatatgccg ctgtaaaata
     6421 gactgcccca atcccctcga gccgctatga accagcagtt gcagatgctg cgcatccaga
     6481 ccagccagtg caaacagttc cgcgtcgata atttgatcaa tctgttgcag ttctgcgaag
     6541 tggttaccgc caccgatgga gccaagcgag ttgcgccacg gatgctgtgc aaatgctgac
     6601 ggcaggtttt cctccagcca gctttcttca gcaacgtcat ccagatctga taatcgcttt
     6661 tcaaacttat cggcgttgta tttgcgagcg agaatatctg tttgccatag cgccatgccg
     6721 cagccgatat cattgccgac cagtgccggg taaaaacgac caacagagaa gaacgctgcg
     6781 ccaatcgggt agccgcgtcc gggatgtaaa tctggcatcc ccacaacgcg ctgcatgttg
     6841 ggtaaatttg ccgtggtgtg taattgttgg atcgctaaac tctcgatcca caggtcatca
     6901 gatgcgatgg taaataccgc atcggataag ggacgaatat atttgcccat gtaccattcc
     6961 atttatgaat aaaaatcagg aaatggcaga cgaatccagg gcaagaaaaa agaattaatc
     7021 ctggaaaggt ctgcaaagag tcattgataa aggatttgtc atgatttacc tccttgcagt
     7081 ttgtggaata agaaaacgcg cacatactag acgggcgtta ttttcattgc aagctggatt
     7141 taatgttgcg gtttatatcg catataaaaa ttagtaaacc acgttccccg gcattcaacg
     7201 cgctgctgct taactgtctg aaaaccataa cgttcaaaaa agggttttgc ggttatgctg
     7261 gcgtccaccg taagttcgga ttcagactta atcaatggtt ttaacaaagc gctggcaacc
     7321 ccgcgtcggg tgtattccgg ttcaacaaat aacatatcga tataatgtcc aatgcgggta
     7381 ataaaaccaa ccggttgtgc attaataact gcaacccgca cttgtgattt cgcgagtttc
     7441 tccttccagc gagattcgtc aatctgcgcc caggcggcaa tttgctgtgg agaatagtgc
     7501 tgactcgccg tcatcgtaac agctcgaagg aaaatagcgc atagttgctg aaaatcacca
     7561 ggctgatagt ttcttatttg tatgttattc ataatataaa ttcaaaaacg catgcgaaac
     7621 gcttctgcca ttttcccaat tttatgcatt tggttgttat ctcgagccag tttatgaggt
     7681 tcaggtttca gaatggcaat gagcaaccat gcttgctcat caaacgcgcc ctgacaatat
     7741 accaaatgcg ctgcgtcatt cgttctgctg aattggcgca actgtggcgg aaatggatta
     7801 ttcacatttg ccagatgaat atgagcaact cgctcaaatt tgattaatgg ccaggtaaac
     7861 gagtcgtcgt agagtgcatc acgaccaaat atatctggca aaacaccgtc acgcttatag
     7921 gaaataaaat ccgccgttaa cgcttcaagt tcctctgctg taagttgcag gcgaataagt
     7981 tttgttttga atacccgcat ccttattcct taaagtcatt gaaatcatca tccgtcatat
     8041 cattaagata ttgttcagcg cggcgtgtaa tttcctctaa tcttgcagca ctcggacgga
     8101 agcgcgcctt tatgggcttt ttctcctctt gttctgcaag catgtccatt aatgcttcaa
     8161 atgcgctggc acttaagaga tatcctgcgg ggcgattatt agaaagaacc gcaacaggtt
     8221 gatcaataaa gtatttagct gggtttttac gtaactcagt gatattgacc gatttttcag
     8281 cgagaattcg atgcatacag tgatctcctc ataataatgc acaccagaat atacaaaata
     8341 gtatacatca taatcccagc accagttgtc tttccatttg cgggtcaagc aacgtcacat
     8401 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     8461 tggcgattag atcagattta ttcagccgcg gccagacgtg ttcctgggtg gtttgctgaa
     8521 aatcgtcgaa ctttaatttc accccctggc gggcaatcag caaatcaggt tttacctttg
     8581 ccaaacgacg ctccagttca gggtaaagcc gctcgatgat cgcttcacat tcagaccagt
     8641 gatgaatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     8701 taacatcgcg ttcgtcaatg ccctgactac gctcccacaa aatgcggcca aatttgccaa
     8761 agcgtttaag cagcatcacc agatcacact tttgcacatc accgcaggtt cgtagcccca
     8821 tcgcttccag tttagccgct gagactttgc cgacgccggg aatttttgcc agcggtaagg
     8881 tttgcagaaa tgccggaacc tcagccggag taatcacaaa ctgaccgttg ggtttattca
     8941 tgtcggaggc gattttggcg agaaacttga ccggcgcaac gcccgcagac gccgtcagtt
     9001 gcagctcgtt gaagatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     9061 aatggacgct atcggtgaca tcgagatagg cttcatccag tgataacggt tcaatgcgcg
     9121 aggtatagcg cgagaaaatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     9181 gccctggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     9241 tacgtacgcc aaatttacgc gcgggataat tggcggtgct gattaccccc cgtcgttcgc
     9301 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     9361 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     9421 tggtaaagta tacagtgatt tcagggtttg agaaatgcgt aaagattcag catttaacgc
     9481 gctcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     9541 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     9601 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg catccagcag catctggtcc
     9661 gccattgcgc ttacctgcat cactttattt tccggcattc cggcctcttc cagcacccga
     9721 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtag
     9781 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaacactggc
     9841 gcaagctcca ccagcaaagt tttaaagaac ggcataatct tcgcgctgcc gcgttcaaac
     9901 atattgcggt tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     9961 tccagattcg cttccatatg cgcatcacgg gcgatggtat tgatactggt cgccagctcg
    10021 ccaagctcgg tggcggattt cttcttcaac agcgccgttt tctcgttgac gtcgcgcgct
    10081 tttttctccg tctcttccgg caccgccacg gtggcaggtt ttggcagatg agacgggctg
    10141 attttattta acggcgacaa cccaccaccg ttaaaaatcg actgcccgtg cagcgcggcg
    10201 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tccacagcgt cataaacagc
    10261 gccatcattg ccaaagtaaa gtcggcgaac gccactttcc acgccccgcc gtggtgcccg
    10321 gcatgattct ttttgatctg ccgccggacg atggtggttt ttgccccacg ctcgcggcgg
    10381 ttgcccgtct ttctcatgct tattcttgct ccagcatcgc attcacccac gcgtcgaggt
    10441 tggcgaaggt cggtttggac gccagatgca gcagcttacg cccggcgtcc actgccagca
    10501 gcgtcggttt gccgcctgcc tgcgccacca gcacggtgcg cacgcattcc agcaaagaat
    10561 gttccgcccg cgcctgttgt tccatggcgt tggcgagcgg gtccatcagg cagtagcaga
    10621 taaagacgcc taaaaacgtc cccaccagcg ctgccgccac tttcaggcca atcagggcga
    10681 tagatccatc aatcgactgc atggtgatga tgatgcccag caccgccgcg caaatgccaa
    10741 accctggcat cgcctcggcg gtacgttgca gcgagcgtga cggcgttaac agcgactctt
    10801 cggcggtgtc cagttcctga tcgagaatcc cttccagttc atgagcgtca atcttgccca
    10861 tcgccatcag gcgaaagtta tcggcaataa aggtcaccag ccgcttctgg gttaacacca
    10921 gcgggtattt ctggaaaatg gtgctctctt ctggttgctc gatatgctga tccaacatgc
    10981 gcagaccacc gttttgcacc atttcgagca actcatacag acacattaat aactggcgct
    11041 gaaactccgg cccgagctgt tttttactga tcacgccttt aatttgatgg gctatctctt
    11101 tcagcacatg cttcgggtta ccaataatca tcgccccgaa gcccgcgcct aaaataatga
    11161 tcattcccgc cggttgccag atagccacta attgcccgcc tgcttcaaaa taaccgccaa
    11221 atacgcagcc gagcaccacc agtaaaccta atattttttg catgatttct tcactttaat
    11281 tcggatagac aaaatcacga attttctgcg cgatctgttt attcagttga caaattctgg
    11341 cctcggttag ccctaatacc agcgcaattt ctttgagatt catctcatgc tggtaataca
    11401 tggagaggat cagttgctct ttttcgctta attgtgaaag cgcctgttgc aacatttgct
    11461 gggtgacaaa ctgatcttcc agttcccggc cagcaagcgg aatgcctggc ccttcgccgt
    11521 tcagcagttc gtcaaggctg gcgagcgttt ccgccccttc cagttgctga tattcgagat
    11581 aatcttcatg cgaaatccct tccacggcca gttctggcca ctccggttcg tgccccagtt
    11641 ttttacgcgt ttcgcgaatc aggtctttca tctgatgata tttctgtcgc agctggcgcg
    11701 gtcgccagtc gagagcgcgc agctcatcaa ggatcgcgcc gcgaatacgg tgcacggcat
    11761 agcccgcaaa accttcgtcc ggcagcccgt agcggcgaat ggcgttaagc agccctatta
    11821 acgccgtttg ttccatatcc tgacggtcaa taatgcaatt acactgcggg gcaaggtgcc
    11881 gcaccacttt acgcaccagt ggtaaatagg cctgcaagta gtggctctcc tgctgcgggg
    11941 ttaatatcgg cgcggcagca aattcttctg actcgaatac gtcctgtagc atgtttatat
    12001 ccgcctggtg attactggaa gaccactttt ttcaccagca aatccttata cggtgcgctc
    12061 attttgcgac gctgaagatc tttctttaac gcgtcagaaa tcagtttgcg gatggcagat
    12121 attttcatgc cgcgcaaatc ttcaaatttc atttccgtca ggcattcaac tgcaatactt
    12181 tgatacagcg gctcctgatg ttttattttt tccgcttctt tgtcactggc aaccaccatc
    12241 actaattccg ccgacaaata atggtccgcg ccgttgttgt catgcaaagt gacaatagtt
    12301 tccggcaacg tcacaaacac gcttttgctt tcatccaggg tttccaccgt ttccgtctgc
    12361 gcggcggcag tcggttttcc ttttccggca taatgatgcc agacacccca tccagctccg
    12421 gcccccacca ccaacgccag aacggcgctg accacgcccg ccaccacgat tttcttcata
    12481 ttgcctttac actttgatca aaacggattc gttctgttga aattgaggcg catcatcaag
    12541 ctgtaaggcg gcactcacct cttcctgctg atggcccgac tgttgttgtt gctgcgactg
    12601 cccttcggag gaaacctgta cgttcacctc cataaaattt tgcgccgtca gatgctggcg
    12661 gagatcgtcg ctaaactgct gtaacgcgcg gcaaacttcg ctttgattcg cgccgatgtg
    12721 caccatcagt ttgccgttgt cgagttgtac ggcgatctcg agcttgccaa gcgacggtgg
    12781 atcgagacgg atggtggaga tctgctgttg tttgttgagc tgaaagtgga tctgatcttt
    12841 caaaagcgtc gtcagttttt cacccagttc gtccatcgaa agcgtggctg gtttggcgac
    12901 aatctcctga tgttcgctga ccagatccgg cgtcttaacc tgcaacacca cacgcgcaat
    12961 aaccggttcg ctgtccggca ctttttccgt tttacgctcg acctgcgggc gcgcggttac
    13021 acgggttagt tcaggtttcg ggcgagctgg ctgtgttgag acgcgcggct gcgtcggcgc
    13081 aatggcatgt aaatcttcac tggcgtaagt cgccagtctg gcctgctgtt cgggtttcgc
    13141 ctgcggcaac tgaccgatca gttcctgcaa ctgcggcggc aacgacgtga gttcctgctg
    13201 ctgcgtcggg cgttgcggcg cgttttgcac caccgctttc gtcaattgct ggataaccgg
    13261 cgatgttgcg acgtttcgcg gctgcggcgt gtcctgatgc ggcgcggcag gttgcggaag
    13321 taacagcgcc attagcgcct gcatcgccac cggatcgttc tcctgcgggc gcgtcgcctg
    13381 atgctgactg gttttagcgc tatgaacgcg ctccgccacc gccgccaccg ccattttcgg
    13441 cagggtaaac gcgggcgcgt tttcaccgct caccggcggt aaaatatccg ctttcagcga
    13501 cgccgtttcc gcaagtgttc cgagcgtggc aagcaatgcc ggattcatga cttctccgaa
    13561 agtaaattaa caattgagta ggccagcacg ccttcctgat gccgctgatg ctgttccata
    13621 cgcgccttca acacttctgc gcgctgctcg cgccgtttaa tcagggtatt aaacgcgccg
    13681 tacaactgcg ctttcaccgt atccaactgc gccgcatcgg tttccgccgc cgcgtaatcg
    13741 tgaaactggc gcatcaactg ctgatgcagt gccggaaggc gtcgccagcg ttgcttgtcg
    13801 agggcttcat tcatcgcctc gaccagtgcg aaaagttgcc tgcgttgttt ttccatcgtc
    13861 ttaacccagc ttgcccgaaa gcccttccca gccttcgcgc aggttgctta agatcagcat
    13921 cacttcatcg attttctctg ccgacagttc gccgctggcc tcatacaatc ggtaaacgca
    13981 gtggtcatag aggcgcgaaa ggttgaccac cagctcgccg ccagtttcaa actccagcga
    14041 actggtgagc gcattgagaa tgtcgatgca tttattgatg ctctgggctt tcttctcgaa
    14101 acgccgccct tcaatatggc ttctggcgcg ttccagctcg tccatcaggc cagaaaacag
    14161 caccagcacc agttccagag gcgatgccgc cgcagtacgc gccgcgagat cgatctcttt
    14221 atattgcgaa tagccgtcct gtacttggta catctttagg gatattcctt ctgaattacg
    14281 cgaatgccgc catactggct ttcatggtgt acacctcaac caacgtattg gtgtactcct
    14341 cgagataacg gtcgtagtta gcgttatagg tatcggtaag ctgatcgcct tcatcctgaa
    14401 ttttgctcat ctgatcatca atgttctgct ggcgcagggt gatgatgccg ttgctggagt
    14461 ccgtgtagat attaatcagg tcgtccatct gcgccaccat gctgttgtcg ccaacgaaaa
    14521 tagaggtcag accgtcagga tttttcgcca tttcgtcgct gaactggtcg gagtcaatct
    14581 gtaagtggcc gtgggaatcg agggtgatgc catagtcaac aatcgacacg ccgttgtagc
    14641 tggcatgggc gatatcatcg agctggtttg ccagtgagct gagtccagca tcccccgcaa
    14701 agaccccggc gctactgctg tcatcaccgt gagtggttag cgaatcaacc gtatcaatca
    14761 aggtgttata ggcatcgaca aaggtctgta ctttctcctg gctggcgctg gaatcttcgc
    14821 tgatgttaaa ggtggtgaga tcgctgtcag aatcgctgac ttcggtaaaa gtcatggtga
    14881 cgccggggat cacatcatca aacgtgttgc tgctgttggt gatagccggt ccgctggcgc
    14941 taccgagatg aataatcgcg tcctgagcgg aggaaacatc cgtggcaacg ggcgcgctgg
    15001 cgctgtcgtt gctggcgtca tgccccgtta cgctcaccga aaatgcgctc tgcgccccgg
    15061 tgctatcgga ggtgagcata atggtggtgg tgccgtcggt ttttaccagc gccgccgaca
    15121 cacccggatt gtcatcagag tcgttaatcg cattcaccag ttcactggcg tcgataaaac
    15181 cgtcgccgtc accgttttga tccgccgccg acagatcaat atccatggtg ctgtcgccca
    15241 tcgtcagttc aaaagtgccg gtagcggaaa aggcgtcgtc gcccatactg aacgtggttt
    15301 gctgcccctg cgccagttgc tcaacgaaaa atgagtagct tcccgcctgc gcctgggaat
    15361 tggcggaaac ggtcgccgag tcattattgc tggtggcggc aaacgtcacc gggccgtcag
    15421 tatcgctgtt cagcgcgtca acggcgctct gaaaatcgct caacgctgag ctgagcgagt
    15481 ccaggccgct gctttcggca tccagctcgc tctgcttctc ctgcaagttt gccgcctgag
    15541 tggcaacatc cgcataggcg atttcttgcg caatggttct tgggttgatc attgatgact
    15601 ccgaataacg atgtcaggtt attggcaaaa ggcgtgccaa tattttttat gtgaagaatc
    15661 aatagattga aaaacaggcg gaaatgacgt ttccggtctg gcggaaaaaa gcctgccgca
    15721 gaaagaacaa caccgcccgc aggcggtgtt gttcgtaggc cggataaggc gtttacgccg
    15781 catccggcaa gtccgccatt actgcagcag gctggaaacc atgctggaca tgctgttgga
    15841 ctgtttcagc atggtgatgc cggtctgcat cagcacctgt tgtttggtca tattggacgc
    15901 ttccgtcgcg tagtcggtat ccataatgtt accgatagcg acttcggtgt tgtcctgcat
    15961 gctggtcagg ttgttggcag tgtcgttcag acggttaata gacgcaccca gcttggattg
    16021 gatctgcgaa acatcatcca tcgcggtaga aatgctggtg atcatctggt tagccgagcc
    16081 agacgcagtc agctcagtac cgccagaaac gcctgcggtg ccagtggtgt cagcctgatc
    16141 cgcactgaag gagttgctga tagcagaaag atccgtcacc agggtattca gctggcttga
    16201 aatattcacg gtcatggtgt cggaagattc cgcgcccacc tggaaagtta ctgcgctctg
    16261 gaacagaccg tcagtaccgg tgttgctggt accggaaaca ccgaacaggt tggtgccgcc
    16321 gtaagtggtg ttttgcagca tgtcagacat ctgctgaccc agttcgtcgt attcgtcctg
    16381 catcgcctgc agatcgtcgt cgctgtaggt gccgttcgct gcctgagttg acaggtcttt
    16441 catacgaccc agcacatcag acatttcgtc gaacatgctg tcggcggttt gcagcatcgc
    16501 ggtggcatca ttgatgttgc tcagcgccac acccataccg ctggactgtg ctgtcagacg
    16561 gttagcaatt tgtttgcccg ctgcgtcatc ggcagaagag ttaatccggt tacccgttgc
    16621 caggcgttcc atagatgtag acagagaaga gctgcttttg ctgattgcat taacggcggc
    16681 cattgaggcg ttattggtgt taatagataa catgatactg ctccttggga ttaatcgttt
    16741 tcgtttcgat accctgtaaa aacgacacct caggagcaaa cttaaaatcg ccgttaaaat
    16801 tttttcgcca cgtattatcc gatgccactt ttagctaaaa aaaagcagag tttaaattca
    16861 gataaaaaga ttattagcaa tatctgccac atggccaggt atcaaaatat gtctttatca
    16921 aatgattgat atagttaaga ttgtcatcaa ttatcgcgtt ttgttctcaa tgtactgatg
    16981 ccaaaattta cattgcgttg caaatagtac acatttggct attgtacctg tcctgtcatt
    17041 tcgttacatt tccactccat atagtgatta agaataatcc tattaccata aaatattaat
    17101 tatcacgcag aaaaatcatc ttctcaggga cgtataagac tgtgctttat gcaaagcact
    17161 atcaggaaac atttatccct tgctaaggta aactcaacta tgagtattat tatcaacaac
    17221 tggcggatgg acccgtcgct taatgcctta atacattgcg aaacgggtga aacacgtcgt
    17281 ctcggcgaat accattttat tttgctggaa acattagcaa aaaatgccga tgtggtttta
    17341 tcgcgatctt atttatgtgc cgaagtatgg aagaaccgca tagtcggcgg taacagcctg
    17401 ccgacggcga tccatgcgtt gcgcgttgct atcgacgatg atggcaaaca acagaacatt
    17461 attaaaacaa ttccgaagaa aggttacttg tgcaataaag agtatgtgtc tttgccggag
    17521 tcatcgcctg cagaagcgct gattattacc gaccagatac aagatacagc cccagaagag
    17581 atatcttcga caacaccgcc tgtacctgtt agaaaaaaac ataaagggct gatggggtta
    17641 gcgttggccg cagctgttat atttattggc tcgaccgtgg ggtattcaca cttgaaaagt
    17701 acacctgacg ccccgcaact ggtaaaagag tcaattaaca gtccaagaat aaaaatattt
    17761 catttaactt ctgctggaga aaataattcc gtaccgttac tatcacaaac cctcgcacca
    17821 ggaaaagaca aactcgataa tttattgtca gcgcataata tgacaatgac aacctattat
    17881 aaatatgttc gtaaccgact ggaaagcgat attgttctgc gtaaccagtg caacggcagc
    17941 tggcaattaa cctttaatgt ggaaggttgg cagaacagcg atattaatag cgcgatgtat
    18001 cagaatttgg agaagttgtt aaatactgtg cagaaatgct gatgagtgcc aatatatgcc
    18061 ggatgcgacg ctaaacgcgt cttatccggc ctaccgataa ttaccccaat aaattcctca
    18121 ccgtctggct ggaaattcgc gcctgaccgt tcacagcctg caataaaacc tgatagttat
    18181 gattggcgct atccagcacg ccgccgagat tttccgacgc ctgcacggcg ctttccgttt
    18241 gcgggaagcg ggacatgccg tcgatcaact gccgcgcctt ttcctgctgc accgccattt
    18301 gcgcgcgctg ctgattaatg ttttctagca tttgagaaat tcccgccccg ccttgttgca
    18361 ggctttgcgc taagcggtcc gcctgtgacg gttcggcaaa ggtttttagc ggcataaacg
    18421 ctgatttatc accatcggta cgcacgctta aggttcgttc gatatggggc cactgttttt
    18481 cagtggtgga gaagctgatg ccatcggcgt gttgatgcat ctgaacgccc acgcggcgca
    18541 gagcgttggt cagccgcgtc tgatactgcg cggcgctgtc atcctcagac agcatcaccg
    18601 ccgaaagctg ggtctgccgc ccgtcagaga cactgaacat gcgcgttcct ggcgtgggat
    18661 tgtgtaccag attcgccaga tccggcgagt ggaaggtcac ccgcgcttcg ccctgcaaca
    18721 ctggttgtaa ctggcggtcc accgcgccgc cggaaagcgc ggcgcgtttg tccagcatct
    18781 gcatcagcgc cgtactctgc gcctgcccgc ccttacgctg gctatgacga taatcgagca
    18841 actgctgttc cagttgcccg agatagtgat ccgcctgctg caacgtggtg agctggtcgt
    18901 taagctgtac gctgtaacgc tgcgggcgag tggtaatcag cggcgaggcc ggatattcac
    18961 tcgccgtcgc cgggagtttt tgccgcaccg cctgagtggg cgcaacggtt gacgacgata
    19021 ccgcagcgct atgtgcgccg ccggtggcga gtgatgaaga tgtaagtccg acctgcatac
    19081 tgttcctgaa agagttagag aacgctgaac aaattcaact cactcacttt cgagtaagtc
    19141 ttgagggtag cttccattgc cacttccagt ccggtaaagg tgatcgacgc tgggccgtaa
    19201 tcgagatcct gcaatgaacc aatcaattca tcattagatg tcgagatatc ggtctgtgca
    19261 tcgctcaaca tcgacatggt gttttgtgtc tcgccgagcg acgcaatcga ggcgttgagg
    19321 tcgtccgagg cggtgtccac cacatcgacg gcgttctgaa tatcgctctg cacctgcgga
    19381 tctgccgggt cgacgtccgg gttttgcagt tcctgcgaca aggaattaag cgtgttaagc
    19441 acgtctaaat tactgccgaa gaaatcgctc gccgccacgt tggtatccac ttccacgccg
    19501 ttcgacacgg tagtctggcg atggtcactg ttaccctgat agctatagct gtcggtcaca
    19561 ccatcaccgt catcatccac agccacaatc ggcggctgat cgttgatggt gccgccaaag
    19621 acgtaatgcc cgtcttcatc ctgatagttc agcgccgcga ccattgattc ggtgagcgac
    19681 tcaatatcct ggccgaggct tgccagcgaa tcggcggtat tggtgccatt cgccgcttca
    19741 agcaggtcgt cgcgtaccgc cagcaggctg ttgttgacgc catcgagaat cgactcctgc
    19801 tggcttaacc ccgccgacgc ggagtcgata ttgctctgat actgctcaat cgccgactgc
    19861 tggcggttta actgagtgat gcgcgtggcg gcgatagggt catctgacgg ctgcaaaatt
    19921 tccttgccgg ttgccatctg ttcaaccaca tgcgccagat cgccggagag attattaaag
    19981 ctttgcgtca tcgagacgta ggtttgttgg gtggttactc gcattttcgc gctcccgctt
    20041 agctgcacat ttccagcacc gaatcgaaaa tctcggcccc ggcggaaatg actttcagat
    20101 tggcttcata aatttgttga taggtgatca ggttcaccgc ctcttcgtcc atgctgacgc
    20161 cgctgacgct gctctgctgg ttttgcgcct cagaataaac attggacgcg gcatcgactt
    20221 ccgtctggtt ttgctggctg taaatgccga tgttgctgat gatcgacgag cacgcctgcc
    20281 cgaccgtcac gctgccaagg ttggctatct ccagcggttc ggtggagatg ttgatcagcg
    20341 cctgaaggtt gtcgctgtta ccgctttcat ccggcgaact ggagaacgcc agctcatcgg
    20401 cggtgatatc cgggttaacg gtcagcgggc catcggcgtt gctggcgtca taaataaaca
    20461 gcggctcgcc ggggttaccg ttgagatcgt agccctgcgc cagctggttg ttgaccgcat
    20521 cggcaaactg caacgccatg ctgttgatgg tgtcggtcag cggcgtcagc acgtcgtttt
    20581 gataatcaaa cagtgcgcct aatgaaccgc cggtgtcggt agtcatcgtc gaggtggtgc
    20641 cagcgaaagt cagcgacatg gtcggcgtgc catcggcatt ggtttccagc gcgatggtcg
    20701 agctttgctg cccgctcacc agcggttgac cgtttttcag ggtgacgttg tagttgccct
    20761 ggtcgtcgat attgacctgc acatccatca tcccgctcag ttcttccacc atctgatcgc
    20821 gggcgtcgta cagcgcggag gcgttatcgc cgttggcttc ggcctgggcg atttgctggt
    20881 tatagctggc gatgccgctg gtaagcgtgt tgatttgcga caccattgcc tgctgctggc
    20941 tgatgatttc cgtgctttgc gagtcgatgt aatccagcgt gttgtcgatg cgcaaggaca
    21001 gcgccccggc ttcgctgatc acctgttcgc gcagggcgga atcatcgggg ctggtggtcg
    21061 cttcgttgag ggcggcgaag aagttatcga agccgccgct caggctgctg ttatcgtcgc
    21121 tcagtaccgc ttccagttgc gtgagatacc cctgctgggt gctgtaatag ccgtaatcgc
    21181 tggcggcata ccacacctga ttcacctgat actggttaga gacgcggcga atgctgtcga
    21241 cctgcacgcc gttaccggcg ctgttcggcg aaccaccgct ggcgccgatg gtgctgatct
    21301 ccgccacctg acgggtgtag cccgtggtca tggcgttggc ggtgttttgc gccgtgacgt
    21361 tcagctctac ctgcgcggtt gatgcgccgc tgtagccgat gttaatcatg tccatgttgg
    21421 atccttaccg gtaaatcgtg ccggttaaaa gcggcgtgaa gatgggggtt cttaagttcc
    21481 gtaaaccggg tgccagacgc cggatgcagc ctgcatacgg gcacaaacat aaaaatatgt
    21541 gcgaactcgt aggcctgata agacgcgtca gcgtcgcatc aggcgttgtg caccgatagc
    21601 cggatgcggc gtaaaccctt atcc
//
LOCUS       DADSZD010000074        21480 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.074, whole genome shotgun sequence.
ACCESSION   DADSZD010000074 DADSZD010000000
VERSION     DADSZD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.074"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            334..786
                     /locus_tag="J1Z28_002468"
     CDS             334..786
                     /locus_tag="J1Z28_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA7717296.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELRRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKS"
     gene            complement(831..1835)
                     /gene="argF"
                     /locus_tag="J1Z28_002469"
     CDS             complement(831..1835)
                     /gene="argF"
                     /locus_tag="J1Z28_002469"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA7717297.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            1997..2413
                     /gene="rraB"
                     /locus_tag="J1Z28_002470"
     CDS             1997..2413
                     /gene="rraB"
                     /locus_tag="J1Z28_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBA7717298.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            complement(2590..3093)
                     /locus_tag="J1Z28_002471"
     CDS             complement(2590..3093)
                     /locus_tag="J1Z28_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7717299.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            3286..4473
                     /locus_tag="J1Z28_002472"
     CDS             3286..4473
                     /locus_tag="J1Z28_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HBA7717300.1"
                     /translation="MNDVNIGKDNSRHSFVFTGKGGEYFLICLVNFLLTIITLGIYGP
                     WALIKCRRYIYQHVTLKGQPFSYKGTGGAIFVSMLLIVVVYLLSISCFAGQHFALGLF
                     LFALLICGIPCMAVKSLQYQANMTSLNDIRFGFNCSMMRAWWVMLGLPVLLALVFWFA
                     LYLIAQVTTSIGGLFFNLVALSLLSAIGLGVVHGITYSKWMPLLGNNATFGIHKFSIQ
                     VNVKECIKGCMLAILTMVPFIIVIGIMIAPVFQQLMMMTMLGRSDAGSEFVLQYYPQI
                     MASYFLYFVAILVFASYLYVTLRNLFLNNLTLANGTIRFHSSVTAIGMLLRMLAVLMG
                     SSITCGLAYPWLKMWMVSWIANNTHVQGDLDSLELTNDDKPQDSGSLMWISRGIMPYV
                     PFI"
     gene            complement(4520..7375)
                     /gene="valS"
                     /locus_tag="J1Z28_002473"
     CDS             complement(4520..7375)
                     /gene="valS"
                     /locus_tag="J1Z28_002473"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBA7717301.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7375..7818)
                     /gene="holC"
                     /locus_tag="J1Z28_002474"
     CDS             complement(7375..7818)
                     /gene="holC"
                     /locus_tag="J1Z28_002474"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBA7717302.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(8076..9587)
                     /gene="pepA"
                     /locus_tag="J1Z28_002475"
     CDS             complement(8076..9587)
                     /gene="pepA"
                     /locus_tag="J1Z28_002475"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBA7717303.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9854..10954
                     /gene="lptF"
                     /locus_tag="J1Z28_002476"
     CDS             9854..10954
                     /gene="lptF"
                     /locus_tag="J1Z28_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBA7717304.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10954..12036
                     /gene="lptG"
                     /locus_tag="J1Z28_002477"
     CDS             10954..12036
                     /gene="lptG"
                     /locus_tag="J1Z28_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBA7717305.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(12197..13699)
                     /locus_tag="J1Z28_002478"
     CDS             complement(12197..13699)
                     /locus_tag="J1Z28_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBA7717306.1"
                     /translation="MSKPLLIARTLDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAAEGTASEKLLARLKNIGVNDWQPHTNPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPTFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(13777..14775)
                     /gene="idnR"
                     /locus_tag="J1Z28_002479"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(13777..14775)
                     /gene="idnR"
                     /locus_tag="J1Z28_002479"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBA7717307.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNAHNHNTVDLGYQIYHGNTL"
     gene            complement(14842..16161)
                     /gene="idnT"
                     /locus_tag="J1Z28_002480"
     CDS             complement(14842..16161)
                     /gene="idnT"
                     /locus_tag="J1Z28_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBA7717308.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(16226..16990)
                     /gene="idnO"
                     /locus_tag="J1Z28_002481"
     CDS             complement(16226..16990)
                     /gene="idnO"
                     /locus_tag="J1Z28_002481"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBA7717309.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(17014..18045)
                     /locus_tag="J1Z28_002482"
     CDS             complement(17014..18045)
                     /locus_tag="J1Z28_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBA7717310.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLLLAKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            18262..18825
                     /gene="idnK"
                     /locus_tag="J1Z28_002483"
     CDS             18262..18825
                     /gene="idnK"
                     /locus_tag="J1Z28_002483"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBA7717311.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(18829..19848)
                     /gene="ahr"
                     /locus_tag="J1Z28_002484"
     CDS             complement(18829..19848)
                     /gene="ahr"
                     /locus_tag="J1Z28_002484"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBA7717312.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQHVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            20044..20128
                     /locus_tag="J1Z28_002485"
     tRNA            20044..20128
                     /locus_tag="J1Z28_002485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20078..20080,aa:Leu,seq:caa)
     gene            20292..>21480
                     /locus_tag="J1Z28_002486"
     CDS             20292..>21480
                     /locus_tag="J1Z28_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA7717313.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDEAKKLLVQGIDPKVVQKEARAENSGAYTFEA
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRIPAKREEIKGVRYSYR
                     GMKMKEEHIVPLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSDDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MMQW"
BASE COUNT         5242 a         5449 c         5348 g         5441 t
ORIGIN      
        1 gcgaaaggat aaggtgatta tttttcacag cactattttc acgggataac tatgacagca
       61 cgggcattaa aagctaagaa aatgaactgt gatctgactg agagttttct tttaacatat
      121 gcaataacaa gatattagga ccaaaataca tagctaagtt ataagattta tgcagcaaaa
      181 ctgcatacaa aattactcct gcttaacatc agtacaacat ctccagcaat aaacatgcgc
      241 aagggcaaaa cctgatgatt tttctatggt gagaggtggc tttttttcgt ataaggaaag
      301 aagtgaaaca ttgttttaat aaaggaagat attatgattg ttggcaacat ccatcactta
      361 caatcctggc tgccggaaga acttcgcgaa gcaattgagt acatcaaatc acatgtcagc
      421 gatgagacag caaaaggtaa gcacgctatt gacggcgacc gcctgtttta tctgatttct
      481 gaggatacga ctgaaccagg tgaactgcgt cgtgctgagt atcatgctcg ttatctcgat
      541 atccagattg tattaaaagg gcaggaaggg atgacattta gcacgcaacc agcaggcgta
      601 ccagagactg actggctggc agataaagat attgctttta tcgggcaggg gatcgatgaa
      661 aaaacagtga ttcttaatga aggggatttt gtcgttttct atcctggcga agttcacaaa
      721 ccgctttgtg cggtgggagc ccccgctcag gtacggaaag cagtagttaa gctattaaaa
      781 tcgtaagtga tcactgccgg gggcgacctg tgccgccgcc cggcaaaacg ttattttgct
      841 aatgtagcga ccataacagc ttttatggtg tgcatccggt tttctgcctg gtcaaagaca
      901 atacttgccg gagattcaaa gacttcatcg gtgacttcca ttccaccata cagaccgaat
      961 tctgcggcca tttttttacc cagcgtggtt tgctcatcat gaaatgcagg cagacagtgg
     1021 aggaacttaa cctgcgaatt accggtcagg gccatcattt tgctattcac ctgatagtca
     1081 cgcagcaggg caatacgctc cgcccatttc tctttgggct cacccatcga tacccagaca
     1141 tcggtataga taaagtctgc gcctttaacc ccagaggcga tatcttccgt caaggtgatt
     1201 ttacccccgt gtttttgtgc cagagcactg cattcggcga ccagcgcagc ttccggccag
     1261 catgcctgtg gcgcgaccag acgcaaatcc agaccggtaa gcgccgcagc ttcgagcatc
     1321 gaattgccca tgttgttacg cgcgtcacct gcatagacca gcgtcatttc gttgaatgct
     1381 ttgccgggca aatgctcctg catggtgagg agatccgcca gcagctgcgt ggggtggaac
     1441 tcattggtca ggccgttcca tactggcacg ccagcgtatt ccgccagtgt ttcgacaatc
     1501 tcctgaccat agccgcgata ctgaataccg tcatacatgc gaccaagcac gcgggcagtg
     1561 tctttaatcg actctttgtg accaatctgg ctgccgcttg ggccgagata agtaacgcga
     1621 gcaccctggt catatgcggc aacttcgaaa gagcatcggg tacgggtcga gtctttttcg
     1681 aagatgagcg cgatgttttt gccagtgagt ctggcttctt ctttaccgct tttcttatcg
     1741 gctttcagct tcgcggctaa ctgcaacaag ctgttgagtt cagctggcgt gaaatcgagt
     1801 aatttcagga aatgcttatg ataaaacccg gacatagatc cctcctgtgg ctaacgcctt
     1861 aatgaattaa aattcaattt atatggatga ttattcattt gcaagtctaa agcataaatc
     1921 tttgtcacaa aggtggaggc aatgtcagtg gtgtgtgaca ataagagtat cggcaggaca
     1981 ttaagaggaa taagccatgg caaacccgga acaactggaa gaacagcgtg aagaaacacg
     2041 tttgattatt gaagaattac tggaagatgg cagcgatccg gacgcgctgt acaccatcga
     2101 acaccatctt tccgcagacg atctggaaac cctggaaaaa gcagcagttg aagcgtttaa
     2161 actcggttac gaagtgaccg atccagaaga gctggaagtt gaagatggtg atatcgtgat
     2221 ttgctgcgac atcctcagcg agtgtgcgtt gaacgccgac ctgatcgatg cccaggttga
     2281 acaactgatg acgctggcgg agaaatttga cgttgagtac gatggttggg gcacttactt
     2341 tgaagatcct aacggcgaag acggcgacga tgaagatttt gtcgatgaag acgatgacgg
     2401 tattcgtcac taattaagcg ttgacgacaa aggctgcgtt acctgatgcg ctacgcatat
     2461 caggcccagg tagttcctgc aacctggtga atttgtagcc cggataaggc atttacgtca
     2521 catccagcat actcaaaaag tacgtggtta atgttcgaat gcccgcgagt ctaagctcgc
     2581 gggcattttt caaagtttgc gtagcattcg cacttcacaa tcaacatgac ccgtgcagcc
     2641 aagcgcatag tcgatatgct caaagcccaa atgctcataa agcgcaatag cttcctttaa
     2701 aaaagcggtc gtttccagat agcagcgttt gaagcccatt tctcgcgcct gctccattgc
     2761 cattaaggcc agtttttttg ccagcccttt gccgcggata gccggaagaa aatacatctt
     2821 ttgcagttcg caaatgtctg actcactccc ggttaatggc gcaatcccac cgccgccgac
     2881 cacttcaccc tcgtactcaa ccacccaata tgcgtggcca ggctgactat acacttgata
     2941 cagctcgtca aggttcggat cggcgacggt gtagccttta tcagcggtaa gaccgtattc
     3001 ggcggatacc tgacgaatga cgtgggcgat agcaggatta tcggcgacgg taagtttgcg
     3061 caaacggagt gcgggagaag cgacaacatt catagcatag ctcaatttca aatgtatgat
     3121 ttatagctca ctttaatacc accctcgctt aggtggtgca agaagaagat aaatcagaaa
     3181 atattacgca tatctctctg agataaagcg tattctaggc gaaccagtat caattgactg
     3241 gaattattat cggtatgaat ggaatttatt ttagggatat agttcatgaa tgatgtaaac
     3301 atcgggaaag ataattctcg gcactcgttt gttttcacag gcaaaggtgg tgagtatttt
     3361 cttatttgtc ttgtgaattt tttactgaca attattactc tgggaatata tgggccgtgg
     3421 gcattgataa aatgccgtcg ttatatttat caacatgtga cactcaaggg gcaaccattt
     3481 agttataagg gaacaggtgg tgccattttt gttagcatgt tacttattgt ggtggtttat
     3541 cttctcagta tttcttgttt tgcaggtcaa cactttgcgc tcggtttatt cctgtttgct
     3601 ctcttgatct gcggtattcc ttgtatggca gtgaagagtc tgcagtatca ggcgaacatg
     3661 acctcactga atgacattcg ctttggcttt aactgctcaa tgatgcgtgc atggtgggtt
     3721 atgctaggac tgcctgttct gttggcgtta gttttttggt tcgcactgta tcttattgca
     3781 caagtgacga catcaatagg ggggttattc tttaacttag tcgcattaag tttgctttct
     3841 gccatcgggc taggtgttgt ccatggtata acctatagta aatggatgcc attgctgggt
     3901 aataatgcaa cttttggcat tcataagttt tctattcagg taaacgtaaa agaatgcatt
     3961 aaaggttgca tgctcgcaat attaaccatg gttcctttca tcattgtgat tggtattatg
     4021 atcgcaccag tattccagca gttaatgatg atgaccatgt tgggtaggtc tgatgctggc
     4081 agtgagtttg tattacaata ttacccgcag attatggctt cttatttcct gtattttgtc
     4141 gcgatccttg tttttgccag ttatctctat gtcacgttac gcaatctgtt tttgaataat
     4201 ctgacgctgg caaatgggac aattcgtttt cattcatctg ttaccgcgat cggtatgctt
     4261 ctgcgcatgt tggcggtatt aatggggtcc tcaattacct gtggtctggc ttatccctgg
     4321 ctgaaaatgt ggatggttag ctggattgct aacaataccc atgtccaggg cgaccttgat
     4381 tcgctggagt taaccaatga tgacaaaccg caggatagcg gttcgttaat gtggatatct
     4441 cgcggtatta tgccgtatgt tccgtttatc taaataaaaa ggccggagca tgctccggcc
     4501 ttcgttttca ctatgagtat tacagtgcgg cgataacagc ctgctgttca atcagcttcg
     4561 ctttcgcttc tgcatagcct tcaagcttct cacgctcttt cgcgatgacc gcttccggtg
     4621 cgcgggcgac aaagccttca ttcgccagct tgttttcgat gcggctgatt tcaccttcga
     4681 tcttcgccac ttctttcgcc aggcgcgcca gctcatcttc tttgttgatg aggccagcca
     4741 tcgggatcag caattcagcc ccgtcgacta tcttagtaac ggaaaccgga cctttgtcat
     4801 cggcaggcag cacggtgatg ctttccagac gcgccagcgt ttgcaggaag ccgcggtttt
     4861 catttacgcg acgttctgca tccgcgctgc aaccacgcag cagcagctcc agcggtttac
     4921 ccggtgcgat gttcatttct gcacggatgt tacgtacagc aacgatcgcc tgtttcagcc
     4981 actcggtgtc ggccagtgcc gcttcatcaa cctgagacgc atcgtactgc gggaacggct
     5041 gcagcatgat ggtgtcggca gtgataccgc aaagtacttt cacacgctgc cagatggttt
     5101 cggtgatgaa cggaatgatc ggatgcgcga ggcgcagcag accttccagt acagtcacca
     5161 gcgtatggcg agtaccgcgc agttcggctt ctgttccacc gttcattacc ggcttggtca
     5221 gctccagata ccagtcgcag aactggttcc aggtgaattc atacagaatg cctgcggcga
     5281 tatcgaagcg gaagctgtcc agcgcttcgc ggtacgcttt gatggtctgg ttgaactccg
     5341 ccagaatcca gcgatccgcc agcgacagcg tcatttcacc gccgttgaag ccgcaatcct
     5401 gaccttccgt gttcatcagc acaaaacggc tggcgttcca cagcttgtta cagaagttac
     5461 ggtagccttc cagacgcttc atatcccagt tgatgtcacg accggtagaa gccagcgccg
     5521 ccagggtgaa gcgcagcgcg tcagtaccgt gcggctcaat gccgttcggg aactgcttct
     5581 cggtgcgctt acggattttg tccgccagct gcggctgcat catattgccg gtacgttttt
     5641 ccagcagttc cggcagcgaa ataccgtcaa ccatatccag tgggtcaatc acgttacctt
     5701 tggatttgga catcttctgg ccttcgtcat cacgaatcag gccggtcatg taaacggtgt
     5761 ggaacggcac ctgtggtttg ccattttcat ctttgatgaa gtgcatggtc atcatgatca
     5821 tgcgggcaat ccagaagaag atgatgtcga agccggatac catcacgctg gttgggtgga
     5881 actggcgcag tgcgtcggtg ttttccggcc agccgagcgt ggagaaggtc cacagcgcgg
     5941 aggagaacca ggtgtcgaga acgtcttcat cctgacgcag cgcaacatca gcaccgaggt
     6001 tattttcttt acgcacttcg tcttcgttgc ggccaacata aacattaccc gcttcgtcat
     6061 accatgccgg gatacggtga ccccaccata gctgacgaga gatacaccag tcctgaatat
     6121 cgcgcatcca ggagaaatac atgttttcgt actgcttcgg tacgaactga atgtcgccgt
     6181 tctcaaccgc ttcaaccgct ggtttcgcca gtacatcggc acgaacgtac cactggtcgg
     6241 tcagcatcgg ttcgataacc acgccgccac ggtcgccgta aggaacggtc aggtcgtgcg
     6301 gtttaatttc ttccagcagg ccgagcgcgt caaccgcagc aacgacggct ttacgtgcag
     6361 caaaacgctc cagtttctgg aactctgccg ggatttcgct ggaataaacg tcagattcgt
     6421 tacctttggt atcgaacacc tgggcgcttt cacggatatc gccgtcaaag gtcaggatgt
     6481 tgatcatcgg cagggcgtga cgtttaccga cttcatagtc gttaaagtcg tgcgctgggg
     6541 tgatcttcac gcagccggtg cctttttcca tgtcggcgtg ttcgtcgcca acgatcggaa
     6601 tacgacggtt aaccagcggc agaatgacat atttgccaat cagatcttta taacgcggat
     6661 cttccgggtt aacggctacg ccagtatcgc ccagcagggt ttccggacgg gtagtcgcaa
     6721 ccaccagata atctttaccg tctgcggttt tcgcgccgtc agccagcgga tagcggatgt
     6781 gccacatcga acctttcgat tcgcggtttt ccacttcaag gtcggagata gcggtgcgca
     6841 gtttcgggtc ccagtttacc aggcgtttgc cacggtaaat caggtcttct ttatacagac
     6901 gaacgaaaac ttctttcacc gcattggaca ggccttcgtc catggtgaag cgttcacgct
     6961 cccagtcgac ggagttgccg agacggcgca tctgacgggt aatggtgccg ccggattccg
     7021 ctttccattc ccagattttg tcgatgaaag cttcgcggcc gtagtcgtga cgggttttac
     7081 cttcttctgc ggcaatcttg cgctcaacga ccatctgggt agcgatcccg gcgtggtcag
     7141 taccgacctg ccacagggtg tttttgccct gcatgcgctg atagcggatc atggtatcca
     7201 tgatggtttg ctggaaggcg tgacccatat gcaaactgcc ggtgacgttc ggcggcggga
     7261 tcatgatgca gaaactttcc tggctttcat cgccattagg cttaaagtag ccctgctttt
     7321 cccagtgctc gtaaagcggc tgttcgatat cttgtgggtt atatgtcttt tccattattt
     7381 ccaggttgcc gtattcaggt tgaaaccagc cacgcggtag gctttatagc gttcgcgcgc
     7441 cagttgtttc agagaatctt cataaggaac gaagtctacc acttctgtga aagcggtggc
     7501 aaaatctgca aagcttgttc gcagactaat caatatatcg cgcgggctgc tgctgcgctt
     7561 ttgcggccag gcgatctcca ccggcgcacc gccgcgcggt ccttctcccg ctaaattatg
     7621 cggaacaaag ctttctgccg gacgcgccca cagggcttca tccagtcggt aagcctgctt
     7681 ttcatcttca caggcgatga gtacgcgctt accactgcgc caacgttctg cggcaatttc
     7741 acacaccaac tgctcaacgg cgcttaagcc atcgacggtg gtgtcattgt ccagaaggta
     7801 gaacgttgcg tttttcatat atggggcttc ttgtggtgga tttaaatacg catcaggtat
     7861 acaccgcagc gcctgatgcg acgctgccgc gtcttatcag gcctacaagt taagtgctgt
     7921 gtgtaggtcg gataaggcgt tcacgtcgca tccgacaata acagccttgc ctgatgcgac
     7981 gctgtcgcgt cttatcaggc ctacgagttc agtgctgtgt aggtcggata aggcgttcac
     8041 gccgcatccg acaataacaa cacagcctga tacaattact cttcgccgtt aaacccggcg
     8101 cggtttagca ggaactgtgc cagcaacgcc accgggcgac cggttgcgcc tttcgcctta
     8161 ccagaacgcc aggcagtacc ggcgatatcc aggtgcgccc agttatactt acgggtaaag
     8221 cgcgacagga agcaacctgc ggtaatcgcc ccaccaggac gaccgccaat gttcgccata
     8281 tcggcaaaat tggactccag ctgttcctga tactcgtcac ccagcggtaa acgccatgcg
     8341 cggtcaccgg attgttcaga tgcggcaatc agctcatggg ccagcggatt gtggttcgcc
     8401 atcagaccag taatatgatg acccagcgcg atcacgcagg caccggtcag cgtcgccaca
     8461 tcaatcaccg cttccggctc aaaacgctca acgtaagtta atacgtcgca cagtaccagg
     8521 cggccttcag cgtcggtatt cagcacttca acggtttgac cggacatggt ggttaacaca
     8581 tcgcccggac gataggcacg cccgccaggc atgttttcgc agcctgccag cacgccgata
     8641 acgttaatcg gcagttgcag ctccgcgacc atccgcatca caccgtaaac cgctgccgca
     8701 ccgcacatat cgtacttcat ctcatccatg ccttctgaag gcttgatcga gataccgccg
     8761 gagtcgaagg ttaaaccttt acccaccagc acgattgggc gtgcatcttc cgacgcgttg
     8821 cctttgtact caatcactga catcagcgat tcgttttgcg aaccctgacc gaccgccaga
     8881 taggaatgca tccccagctc tttcatctgc tgttcgccga taactcgggt gatgacattc
     8941 ttgctgtagc tgtcagccaa ctggcgcgcc tgagaggcga ggtaagcagc gttacagata
     9001 ttcggtggca tattgccgag atctttcgcg gctttaatcc ctgcggcaat cgccagacca
     9061 tgctggatcg cgcgttcacc gctggtcagc tcacggcggg tcggcacgtt gaacaccatt
     9121 ttacgcagcg gacgacgcgg ttcgctcttg ttcgttttca actgatcgaa actgtagagc
     9181 gtctcttttg ccgtctcgac agcctgacgc actttccagt agttgttacg gcctttaacg
     9241 tgcagttcag tcagaaagca gaccgcttcc attgagccag tatcattcag cgtattaatg
     9301 gttttctgaa taacctgctt gtactgacgc tcatccagct cacgttcttt gccgcaacca
     9361 ataaggagaa ttcgctcgga aagtacattc ggcacatggt gcagcaacaa tgtctgcccc
     9421 ggttttcctt ccagttcgcc ccgacgtagc agggcgctga tgtacccatc gctgatttta
     9481 tcgagctgtt ctgcaatcgg agaaaggcga cgtggttcga agacgcccac gacgatgcag
     9541 gcactccgct gtttctccgg gctaccgctt tttacactaa actccatgca ctacgctcct
     9601 gaatcttaaa gacaacggcg gtggctacag atagaattgc aagctttcgt aactcatgtc
     9661 cgctgttgcg atgacttcgt gttaatctta acgttattac ggcattggca cgtcagaaca
     9721 aaatctgaga ggtgaatccg ttgagtataa ttatcttagc gacgatttcg acgactcaag
     9781 agaataaatg acgtttaagc catgaaacaa gctaaaatcc tgcaaaagac gagtttttac
     9841 gggcgtattt aaagtgataa tcataagata tctggtgcgg gagacgctca aaagccagct
     9901 ggcgatactc ttcatcttgc ttttgatctt cttctgtcaa aagttagtga ggatcctcgg
     9961 cgcagcggtt gacggcgata ttccggcgaa tctggtgctc tcccttctcg ggttgggcgt
    10021 gccggaaatg gcgcagctta tcctgccatt aagcctgttc ctcgggctgc tgatgacgct
    10081 gggcaaactg tataccgaaa gtgaaattac ggtaatgcat gcctgcggtc tgagcaaagc
    10141 ggtgctggtg aaagcggcaa tgatccttgc ggtgttcacg gcaatcgtcg cggcggttaa
    10201 cgtgatgtgg gcgggaccat ggtcatcgcg tcatcaggat gaagtgttag cagaagcgaa
    10261 agcgaaccct ggcatggcgg cgctggcgca agggcaattc cagcaagcga ctaatggcag
    10321 ctcggtgctg ttcatcgaaa gcgttgacgg cagcgatttc aaagatgtgt tcctcgcgca
    10381 aattcgacca aaaggtaatg cacgaccttc tgtggtggtg gccgattccg gacatttaac
    10441 ccagctgcgc gacggctccc aggtcgtcac tctcaaccag ggaacgcgct tcgaaggcac
    10501 tgcactgtta cgtgatttcc gcattacgga tttccaggat tatcaggcga tcattggtca
    10561 ccaggcggtg gcgctcgacc cgaacgatac cgaccagatg gacatgcgca cattgtggaa
    10621 caccgacacc gatcgtgctc gcgcagaact gaactggcgt atcacgttgg tgttcaccgt
    10681 gtttatgatg gcacttatgg tcgtaccgct gagcgtggtt aacccgcgtc aggggcgcgt
    10741 actatcgatg ctgccagcca tgctgctgta tctgttgttc ttcctgatcc agacctccct
    10801 gaaatcgaac ggcggtaaag gtaagctgga cccgacactg tggatgtgga ccgttaacct
    10861 gatttatctg gctttggcga ttgttctcaa cctttgggac accgtgccgg tccgccgcct
    10921 gcgcgccagt ttttcgcgta aaggagcggt gtgatgcaac cttttggcgt acttgaccgc
    10981 tatatcggta aaactatttt caccaccatc atgatgacgc tgttcatgct ggtgtcgctg
    11041 tccggcatta tcaagtttgt cgatcagctg aaaaaagccg ggcaggggag ttacgacgcg
    11101 ttaggcgcag gaatgtatac cttgctgagc gtgccgaaag atgtgcaaat cttcttcccg
    11161 atggcggctc tgcttggggc gttgcttggt cttgggatgc tggcgcagcg cagcgaactg
    11221 gtggtgatgc aggcttctgg ttttacccgt atgcaggtgg cgctgtcggt gatgaaaacc
    11281 gccattccgc tggtcttgct gacgatggcg attggtgaat gggtcgcgcc gcagggcgag
    11341 cagatggcgc gtaactaccg tgcgcaggcg atgtacggcg gctcgttgct ctctacccag
    11401 caaggtttat gggcgaaaga tggcaacaac ttcgtctaca ttgagcgggt taaaggtgac
    11461 gaagagttag gtggcatcag tatttatgcc tttaacgaga atcgtcgtct gcaatccgta
    11521 cgctatgccg ctaccgcgaa gtttgacccg gaacataaag tctggcgtct gtcgcaggtt
    11581 gatgaatctg atttgaccaa tccgaaacag atcaccggtt cgcagacggt gagcggcacc
    11641 tggaaaacca acctcacgcc agacaaactg ggcgtggtgg cgctggaccc ggatgcactc
    11701 tctatcagcg gtttgcacaa ctatgtgaag tatctgaagt cgagcggtca ggatgccgga
    11761 cgttatcagc tcaacatgtg gagcaaaatc ttccagccgc tatccgtggc ggtgatgatg
    11821 ctgatggcgc tgtcgttcat ctttggccca ctgcgtagcg taccgatggg cgtgcgtgtg
    11881 gtcactggta ttagcttcgg ttttgtcttc tacgtactgg accagatctt cggcccgctg
    11941 acgttggttt acggcatccc gccaatcatc ggcgcactgt tgccaagcgc cagcttcttc
    12001 ttaatcagcc tgtggctgtt aatgagaaaa tcgtaacctg atgaacgaat aaagggagcg
    12061 aaagctccct ttattattgt tagcaaagtg tgcttcgttc atgcctgaaa aatcatgaaa
    12121 attcaatata ttgcagtggt gtgggcatga taaggcgcgc cagcgtcgca tcaggcaaag
    12181 acaaatcgca cctggcttac cttcttctcc cccccagcaa actccccaac atcccgcgca
    12241 caatctgatt cgtcacctgg cgggcggcgc tttttgccat tgtttgcacc acaccatctt
    12301 tcttcccgcc gcgtggtccg gtagtgccaa acaaaatatc cttcaatcca ccgagaatac
    12361 cgtcatccac cgccacctct ttccctttcg cgggaggatt attttgctgc tcgatactgg
    12421 cctgaaagcc tttttgcaac atctcatagg cggattcccg gtccacgtca tcctcgtact
    12481 tgccatacac cggggagtgg ttaatcaagc cattgcgctc atcttccgtc accggcccca
    12541 tccgcgaaca aggcgcaatc accatcgccc gttccaccac agaaggactt cctttcgcat
    12601 cgagaaacga gattaacgct tcgcccgtcc ccagttcctg gattgccttt tcggtatcaa
    12661 acgtcggatt ggcccgcatg gtttgcgccg ctgccttcac tgctttctga tctttaggcg
    12721 taaaagcacg caaagcgtgt tgaacgcgat taccgagctg cccgagcacg ttatccggga
    12781 tatctgacgg gttttgcgaa acgaaccaga cgcccacgcc ttttgagcga ataagcctta
    12841 tcacctgctc aatcttatcc agcagtacct gcggtgcatc gttaaacagc agatgcgctt
    12901 cgtcgaagaa aaacaccagt tttggtttct ccagatcgcc cgcttccggc aattgttcat
    12961 ataactctga aagcatccac agcaggctgg cggcgtacag tttcggcatc tgataaagtt
    13021 tctcggcgct gaggatattg ataacgcctt taccgttggc atcggtgcgc atccagtctt
    13081 tgatatccag catcggctca ccaaagaagt gtgctgcgcc ttgctgttcc agcgacagta
    13141 atccgcgctg gatggcacca accgatgcgc tactgatatt tccgtactga ttctggaagg
    13201 atttggcgtt atcgccgatg tactgggtaa tcgcccgcaa atctttaaag tcgagcagca
    13261 acaatccctg atcgtccgca atgcggaaga taatattcag cacgccagat tgcacatcgt
    13321 tgagattcaa cagtcgtgcc agcaacagcg gccccagatc cgaaaccgtc gcccgcaccg
    13381 ggtggccttt ctcgccaaag atatcccaca ccaccaccgg attagtatga ggttgccagt
    13441 catttacgcc gatattttta agccttgcga gcagtttttc cgacgccgta ccttctgccg
    13501 caaccccggt taaatcacct tttacatcgg ccataaacac cggaacgccg atttctgaca
    13561 gagattccgc aagtttttgc agcgtgacag ttttacctgt ccccgttgcg ccagtaatca
    13621 gcccgtgacg gttagccatt cccggcagta aaaacagttc tgtgtccagc gtgcgggcaa
    13681 ttaacagggg tttgctcatg agatttcctc cagtttcctg cctggagtat aggcaacgtc
    13741 cggcaaagag aatatgtgta atgaagagaa aataacctaa agcgtgttac cgtgataaat
    13801 ctgatacccg aggtcaacag tgttgtggtt gtgagcgtta ttcttaattt tgctcagcaa
    13861 catttgggcg gccatccgac caatatcaaa acggggagta attacgctgg cgaggcttgg
    13921 gatcatttgc ctgccaatct ccagcccatg aaaacccgca atggaaattt gttctggcac
    13981 ggcgaggttt cgctcgcggc acagcagaag cgcacccatc gcaatatcgt cattggtgca
    14041 aaatacaccg tctaaatccg ggtttgcgct aagggcatca cgcattaact gcattcctaa
    14101 atgaatggat gagatggcgc gtggattcat gcgtaatggg gaaagattat gcagcatcat
    14161 cgcatcgcaa tacccctgat aacgctgttc atcgcgggtg tcatctttgg aaccgagata
    14221 cagaattttg cgcctgactc gcttatccag catggtacac accatgtcaa aagcagcctg
    14281 ccgattatca aaaccgacct ccatatccag ccgttctccc tgtacatcca tcaattccac
    14341 gaccggaatg gtggctgaac gcagaaattt caccgtcctg atggtgtgat atttttccga
    14401 aagaataatc ccgtcaatgt tataagagag caggttaatc accgactctt cttcggaatc
    14461 gcgatcgtaa ttgtaattag caataagggt ctgataatta tgcacagagg tgacggattc
    14521 tattcccgcg agaatgtcag cgaagagttg attttgaaat gacggaatca agataccgag
    14581 ggtataactt tgtgcattca acaacatgcc gggagcgcga ttagggatat aattgatctc
    14641 ttccataatt ttggcgatgc gctcgcctgt ttcctttgcc acctttttcg gcgagcggat
    14701 ataacgactc acggtcattt ttgttacgcc agccagcgta gcgatatcct gtaaagaaat
    14761 tctgtgattg cgcataactt ctcccaacgt cgggggaatc ttcaatcttc ccccgaaact
    14821 acatcacaat attttattct ttcagtgcaa tacggcgtta atggctaaca cgcccagcaa
    14881 acccataata gaaataaggg tttccatcac cgtccaggta cgcaacgttt caccaaccgt
    14941 cagattaaaa taccctttaa atagccagaa gccagggtcg tttacgtgtg acgcgatcac
    15001 gctgcccgca ccggttgcca gtaccattaa agcgggatcg gcatgggtaa cgttgataat
    15061 cggcaacacc acacccgcgg tggtaatggc cgcgacggtg gcagagccca gagcgatacg
    15121 caacagcgcc gcaacagtcc agcacatcaa taacggcgaa agcgtagttc cggtcattaa
    15181 gtgtgaaata tactgcccga caccgctatc tactaatacc tgcttaaacg cgccgccgcc
    15241 agcgataata aacacaatca tcgcgatagc gcctatagaa tccccaatga tatccatgat
    15301 ttgctcgatg gtgcgtccat tacgtcggcc cagtgtgaaa atcgcaataa caatggcaat
    15361 aaacagcgca acggcagggt taccgacaaa ttcaaagaag aggcgcacgg tgttagtttt
    15421 cggtaacgta atttcacaaa cggcggcgat agccatcagg atgaccggga tcacggcagc
    15481 gaaaatactg ttccagaagg agggcatctc ctcttcgcta aacagatgag gattaaataa
    15541 gccttccggt ggtgctttct caaagcgagt tagcagttta gaaaacagcg gtcctgcgac
    15601 aataactgtc ggaatggtaa tgataaatcc atacagtaga gtcgttccga gattagcctc
    15661 aaagatagtc gcgatggcag taggccctgg atgtggcggc agaaaacagt gggttacaga
    15721 gagcgctgct accatcggta cgccaacata caacaggggt aatccagatg atgccacgat
    15781 ggtaaatacc aacggcagca gcaggacaaa acccacttca aaaaacatgg cgaggccgac
    15841 aaccagaccg gtgatcacta gcgcccattg cacgcgtttt ttaccaaaag tagcaatcag
    15901 cgtagtggcg atacgttgtg ccgcgcccgt atcagaaatc agcttgccta acatggcccc
    15961 gaaaccgagg atcattgcca gcccgccgag cgtgctgccg ataccatttt gtatagagtg
    16021 caggacggcc tgtgcatcca tcccttcggc aaatccgacg acggcagcga ccagaacgag
    16081 ggcaataaag ccgttaactt taaagccgat catcaggata agaagcagcg cgacgcctgc
    16141 cgcaataatg attaatggca tagtctttat ctcttattag tgccagaaaa gcagcgcttt
    16201 cgcgctgcac gtagggtggt ggctgttaaa cagccactaa catgccgcca tcaacaaaca
    16261 acaggtggcc gtttacgaaa tcagaggctt ttgaagaaag gaacaccgca gcaccaatca
    16321 gttcctgcgg atcgccccag cgtgcggcgg gggtccgttt gcacaaccag gcggtgaagg
    16381 cttcgtcctc aaccagtgct ttagtcattt ctgttttgaa atagcccggc gcaataccgt
    16441 tgacctgaat attgtggcgc gccagctcga cgcacatgcc gcgggtgagc atttttaccg
    16501 ctcctttcga tgcggcataa ggggtgatgg tgtcacgtcc cagttcgctt tgcatcgagc
    16561 aaatattaat aactttaccc gccttgcgtt caaccatgtg acgagttacc gcttgcgaca
    16621 ccaggaacac ggcggtctgg tttactgcga tcacatcatt ccactcttgt tcagggaact
    16681 cagtaaaagg atgacggcgc tggataccgg cgttattcac cagcacatca atggggccga
    16741 tgtccttttc gatatgttca acggcggcat caatttcatg tttatgagta acattaaaag
    16801 gtgcggcaac ggcctgaata ccctcctggt ggagtttttc tacagcaagt tctgcgcgtt
    16861 cggcagtaat atcattaata attatttgtg cgccatattt acccaggccg gttgccagta
    16921 aatagccaat gccctgtgct gaaccggtaa tcaagatatt ttttcctgcc agtgaaaata
    16981 gatcgttcat tgtattattc ctttatttat ctcttagaaa acaagctgga cttttgctgc
    17041 ctgggtttta tcaccggcga aacgtagcgc ctcttccagg tcagtgaaga gatattcagc
    17101 actcagtaaa ggcagtggat tgataacgcc attcgccagc catgacactg cggtattaaa
    17161 ttcgctggta aaacggaaag agcctttgag tgaaatctcc ttaccaatca acgtcatcat
    17221 tgggaattct gccatcgcgc ctcccatccc tacctgcacc attacgccgc gtgcacgagt
    17281 gacctccaga caggtattca ctgatgaagg atgaccggac acttcaaagc tgacatcgaa
    17341 atagcctttt tccgctttcc agtgatccat atcgtcgttt tgtgggttta cgagcacatc
    17401 cgcccccatc tctttggcca gcaaaaggga acggggactc acatcagcac agacaatttc
    17461 cgcggccccc agtgttttca ctgcactgac aatcaggcag ccaatgggcc caacaccgga
    17521 aataaatact cgcttgccct gtaactcgcc ggcctgatgt gcggcatgaa tcgcgacggc
    17581 taaaggttcg gcaaaagcca taaccttttc gtcagctttg gccggataag ggacacattg
    17641 cgacgtttcg accattttat aacgggtaaa gccaccatca acatgaggga aatacatggc
    17701 actgccaaaa aaacgcatat ctgtacactg attctcgtta tgttcaatgc aatatttgca
    17761 gtgaccgcac ggtttagacg gattaatggc taccgtttgc ccttcatgta attctgatga
    17821 gtcgctatga ataactttac cgataacttc atgacctaac accatcggtg cctttatcat
    17881 gaaattacct acttttcctt cctgataata atgtaaatcg gaaccgcaaa ttccacctcg
    17941 ggttatttgt actaatgttc cattattatt ccaatctatc gtctgctcgg taacggcaac
    18001 agttttcttg cccgcaacaa cgcaggactg tgttttcact tgcataaatt cctcactggt
    18061 caggtagaca cctcggaagc atttaagcgg ttttaattgt cattatttgt gatgaagatc
    18121 acgtcagtaa attgttacat tactatgtta cgcataacgt gatgtgcctt gtaattctta
    18181 tcaacagaaa taaaaaaacg tgaaattatt atgccgccag gcgtagtatc gccgcaggta
    18241 agatgattca ggagatttta aatggcgggt gaaagcttta ttttgatggg cgtatcaggg
    18301 agtggtaaaa cattaattgg tagcaaggtt gccgcgttat tgtctgcgaa atttattgat
    18361 ggtgacgatc ttcatccagc caaaaatata gataaaatgt cgcaaggcat tccattatct
    18421 gatgaagatc gacttccctg gctggaacgc ttaaatgatg cttcatacag tctttataaa
    18481 aagaatgaaa caggatttat tgtctgttca tcattaaaaa aacagtatcg tgatatttta
    18541 cgcaagggta gcccccatgt tcattttctc tggttagatg gcgattatga aactattctc
    18601 gcgcgaatgc agcgtcgggc tgggcatttt atgccggtag cgttactaaa aagtcagttt
    18661 gaggcgctgg agcgtccaca agcagatgaa caggatattg tgcgcattga tatcaaccat
    18721 gatattgcca atgtcaccga acaatgtcgg caggctgtgc tggcgataag acaaaacaga
    18781 atatgtgcga aagagggcag cgcctcagat cagcgctgcg aatgattttc aaaaatctgc
    18841 tttcaacacc acgcggtagc gcgctttacc atcgcgcaca tgctggatgg cgtcgttaat
    18901 tttcgacatc gggaacagtt cggtagtcgg cgcaaccttg ctgcgagcgg caaagcgcat
    18961 cagcttacgc aactcataag gtgtaccggt tgctgaacca gagacgctgc ggtcgcccgc
    19021 aattaacgta aaggccggaa cagacagcgg cgtgaggacc gcaccgaccg tatggaaatt
    19081 accgccatag gtcagcgcct caaaataggg ctgccagtcg aggctgacgt tgacggtgtt
    19141 gataatgaga tcaaactgcc ccgccagtgc tttcagtgcc tgtggatcgc ggctattcac
    19201 cactttatcg gcacccatcg ccagcacttc ctgctctttc gccggattag aactaaaggc
    19261 tgtcacctcg catcccattg cgtgcagaag ttttatagct atatgcccca gcccgccaat
    19321 accaattacc ccaacgcggc tggtggcagt gatatggtgc atcaacagcg gtttaaagac
    19381 cgtgataccg ccgcacaaca gcggtccggc agattcaatg tcgatatttt ctggcagtgg
    19441 aatcacccat tgccagtccg cacgcaactt ctcggcaaag ccaccgcgat tcataatcgt
    19501 cggcaccgca ccttgctcgc agttgatctg attaccgcta atacaggcat cgcaatgccc
    19561 gcagctgcgt gccgtccagc caatcccgac atgctgaccg acctgcaaac ctttatcctg
    19621 cgcggcactc ccgagcgcca ctacgcgacc aatcacctca tgcccggcaa ccagtggata
    19681 ttgtgaaaat ccccattcgt tatcgatcat tgacagatcg gaatgacaga tcccgcagta
    19741 atccacctgc acttcaacat cttgtggctt cagttcaccg ggatcgtact cataaacttc
    19801 cagttcgcca cccgcttctt ttgcggcata gctttttatc atcgacattt tttggtcctt
    19861 ctctggtgtg gtctgggaag tgtagagcat ggcagggcgg ggggtctgga gtgtgacaaa
    19921 ggttacacat cgctgtatgc aatgctgaaa atttcagcac ttagcgagat gcgagcaagc
    19981 tggcgcttgc atggtggcgt gcgacaggta taatccacaa cgttttccgc atacctcttc
    20041 agtgccgaag tggcgaaatc ggtagacgca gttgattcaa aatcaaccgt agaaatacgt
    20101 gccggttcga gtccggcctt cggcaccatt cttacttcca agaccatccg agaaagtcca
    20161 aatatccctt taaaatcaat gcttgcagcg attttttcgt cctgcgtctt ccgaggttgt
    20221 ccgttgaaat ccggatatca ttgggggcat aattgggggc atcttaactt cgattagaaa
    20281 tgtgccccca aatgaagctc aacgccagac aggtcgagac cgcaaagcca aaagacaaaa
    20341 cctacaaaat ggccgatggt ggtggtttgt atcttgaggt ttcggccaaa ggttccaaat
    20401 actggcgcat gaaatacaga cgtccctctg acaaaaaaga ggatcgtctt gcgtttggtg
    20461 tttggcctac tgtgacgctt gctcaggcaa gagcaaaacg cgatgaagct aaaaagcttt
    20521 tagtacaggg catagaccca aaagtcgtac aaaaagaagc tcgggccgag aattcggggg
    20581 catatacttt tgaagctatc gcccgagaat ggcatgccag taacaagcgc tggagtgaag
    20641 accatcgatc gcgcgttctt cgctatcttg agctttatat attccctcat atcggttcgt
    20701 ccgacattcg gcagcttaaa accagccacc tgttagcccc gattaaaaaa gttgatgcca
    20761 gtggcaaaca cgatgtcgct cagcgcctgc aacagcgcgt cacagccatt atgcgttatg
    20821 ccgtacagaa cgattacatc gactcaaatc cagccagcga tatggctggt gcgctatcga
    20881 caaccaaagc gcgacattac cctgctttac cttctagccg attccctgag tttcttgcac
    20941 gtcttgctgc atatcgtggc cgtgtaatga cacggattgc ggtcgagctt tccttgctaa
    21001 cttttgtgcg ttccagtgaa ttacgtttcg cacgttggga tgaattcgac ttcgataaat
    21061 ctctttggcg tatacctgca aagcgagaag aaattaaagg tgtgcgttat tcgtaccgag
    21121 gcatgaagat gaaagaggaa catatcgttc cgcttagtcg acaggcgatg attttattaa
    21181 accagttaaa gcagattagt ggtgataaag agctgctttt tccgggggat catgacgcaa
    21241 ctaaggttat gagtgaaaac acggtaaaca gcgcattgcg tgcgatgggc tatgatacta
    21301 aaaccgaggt gtgtgggcat gggtttagga ctatggcgcg tggtgcgttg ggggagtcgg
    21361 ggttatggag tgatgacgcg atagagaggc aactgagcca ctcagagcgt aataatgtac
    21421 gtgcggcata tatccatacc tccgagcatt tggatgagcg gcgtttaatg atgcagtggt
//
LOCUS       DADSZD010000075        21422 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.075, whole genome shotgun sequence.
ACCESSION   DADSZD010000075 DADSZD010000000
VERSION     DADSZD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.075"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..2859
                     /locus_tag="J1Z28_002487"
     CDS             <1..2859
                     /locus_tag="J1Z28_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7717314.1"
                     /translation="QLKTTNDNVSTNTTNITTNTTNITNLTDSVGDLKDDSLLWNKAA
                     GAFSAAHGTEATSKITNLLAGKISSNSTDAINGSQLYGVADSFTSYLGGGADISDTGV
                     LSGPTYTIGGTDYTNVGDALAAINTSFSTSLGDALLWDATAGKFSAKHGINNAPSVIT
                     DVANGAVSSTSSDAINGSQLYGVSDYIADALGGNAVVNTDGSITTPTYAIAGGSYNNV
                     GDALEAIDTTLDDALLWDTTANGGNGAFSAAHGKDKTASVITNVANGAVSATSSDAIN
                     GSQLYSTNKYIADALGGDAEANADGSITAPTYTIANTDYNNVGEALDALDNNALLWDE
                     DAGAYNASHDGNASKITNVAAGDLSTTSTDAVNGSQLNATNILVTQNSQMINQLAGNT
                     SETYIEENGAGINYVRTNDTGLTFTDASAAGIGSTAVGYNTVAKGDSSVAMGYNSFAK
                     GDSSVAIGQGSYSGVDTGIALGSSSVSSRVIVKGSRNTSVSEEGVVIGYDTTDGELLG
                     ALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAV
                     GEDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTT
                     RGAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQV
                     SQNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSVA
                     IGSGSIAAADNSVALGTGSVANEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKS
                     SEAGGVRYDTKADGSIDYSNITLGGGNSGTTRISNVSAGVNNNDAVNYAQLKQSVQET
                     KQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESA
                     VALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            3269..4924
                     /gene="lldP"
                     /locus_tag="J1Z28_002488"
     CDS             3269..4924
                     /gene="lldP"
                     /locus_tag="J1Z28_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBA7717315.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4924..5700
                     /gene="lldR"
                     /locus_tag="J1Z28_002489"
     CDS             4924..5700
                     /gene="lldR"
                     /locus_tag="J1Z28_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBA7717316.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTKQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            5697..6887
                     /gene="lldD"
                     /locus_tag="J1Z28_002490"
     CDS             5697..6887
                     /gene="lldD"
                     /locus_tag="J1Z28_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBA7717317.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
     gene            6935..7408
                     /gene="trmL"
                     /locus_tag="J1Z28_002491"
     CDS             6935..7408
                     /gene="trmL"
                     /locus_tag="J1Z28_002491"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBA7717318.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(7461..8282)
                     /gene="cysE"
                     /locus_tag="J1Z28_002492"
     CDS             complement(7461..8282)
                     /gene="cysE"
                     /locus_tag="J1Z28_002492"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBA7717319.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8362..9381)
                     /gene="gpsA"
                     /locus_tag="J1Z28_002493"
     CDS             complement(8362..9381)
                     /gene="gpsA"
                     /locus_tag="J1Z28_002493"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBA7717320.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9381..9848)
                     /gene="secB"
                     /locus_tag="J1Z28_002494"
     CDS             complement(9381..9848)
                     /gene="secB"
                     /locus_tag="J1Z28_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBA7717321.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(9912..10163)
                     /gene="grxC"
                     /locus_tag="J1Z28_002495"
     CDS             complement(9912..10163)
                     /gene="grxC"
                     /locus_tag="J1Z28_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBA7717322.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10304..10735)
                     /locus_tag="J1Z28_002496"
     CDS             complement(10304..10735)
                     /locus_tag="J1Z28_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBA7717323.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            10979..12523
                     /gene="gpmM"
                     /locus_tag="J1Z28_002497"
                     /gene_synonym="pgmI"
     CDS             10979..12523
                     /gene="gpmM"
                     /locus_tag="J1Z28_002497"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBA7717324.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRTFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASQVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12533..13816
                     /gene="envC"
                     /locus_tag="J1Z28_002498"
     CDS             12533..13816
                     /gene="envC"
                     /locus_tag="J1Z28_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBA7717325.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13820..14779
                     /locus_tag="J1Z28_002499"
     CDS             13820..14779
                     /locus_tag="J1Z28_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBA7717326.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(14766..15800)
                     /gene="waaH"
                     /locus_tag="J1Z28_002500"
     CDS             complement(14766..15800)
                     /gene="waaH"
                     /locus_tag="J1Z28_002500"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBA7717327.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RIARSAFNLR"
     gene            complement(16039..17064)
                     /gene="tdh"
                     /locus_tag="J1Z28_002501"
     CDS             complement(16039..17064)
                     /gene="tdh"
                     /locus_tag="J1Z28_002501"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBA7717328.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17074..18270)
                     /gene="kbl"
                     /locus_tag="J1Z28_002502"
     CDS             complement(17074..18270)
                     /gene="kbl"
                     /locus_tag="J1Z28_002502"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBA7717329.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            18484..19416
                     /gene="rfaD"
                     /locus_tag="J1Z28_002503"
     CDS             18484..19416
                     /gene="rfaD"
                     /locus_tag="J1Z28_002503"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBA7717330.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            19426..20472
                     /gene="rfaF"
                     /locus_tag="J1Z28_002504"
     CDS             19426..20472
                     /gene="rfaF"
                     /locus_tag="J1Z28_002504"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBA7717331.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNQILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            20476..>21422
                     /gene="rfaC"
                     /locus_tag="J1Z28_002505"
     CDS             20476..>21422
                     /gene="rfaC"
                     /locus_tag="J1Z28_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBA7717332.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQIVCRAPGNELSQLTANAVKQF"
BASE COUNT         5220 a         5657 c         5607 g         4938 t
ORIGIN      
        1 cagctcaaaa ccaccaacga taacgtgtcg accaacacca ccaacatcac cactaacacc
       61 accaacatca ccaacctgac ggattccgtt ggcgacctta aggacgattc tctgctgtgg
      121 aacaaagcgg ctggcgcatt cagcgccgcg cacggtaccg aagctaccag caagatcacc
      181 aacttactgg ctggcaagat atcttctaac agcactgatg ccattaatgg ctcacaactt
      241 tatggcgtag cggattcatt tacgtcatat cttggtggtg gtgctgatat cagcgatacg
      301 ggtgtattaa gcgggccaac ctacactatt ggtggtactg actacactaa cgtcggtgat
      361 gctctggcag ccattaacac atcatttagc acatcactcg gcgacgccct actttgggat
      421 gcaaccgcag gcaaattcag cgccaaacac ggcattaata atgctcccag tgtaatcact
      481 gatgttgcaa acggtgcagt ctcgtccacc agcagcgacg ccattaacgg ttcacaactt
      541 tatggtgtta gtgactatat tgccgatgct ctgggcggga atgctgtggt gaacactgac
      601 ggcagtatca ctacaccaac ttatgccatc gcgggtggaa gttacaacaa cgtcggtgac
      661 gcgctggaag cgatcgatac cacgctggat gatgctctgc tgtgggatac aacagccaat
      721 ggcggtaacg gtgcatttag cgccgctcac gggaaagata aaactgccag tgtaatcact
      781 aacgtcgcta acggtgcagt ctctgccacc agtagcgacg ccattaatgg ctcacagctc
      841 tatagcacca ataagtacat cgctgatgcg ctgggtggtg atgcagaagc caacgctgac
      901 ggttctatca ctgcgccgac ttacaccatt gcaaataccg attacaacaa cgttggtgaa
      961 gccctggatg cgcttgataa taacgcgctg ctgtgggatg aagacgcagg tgcctacaac
     1021 gccagccatg atggcaatgc cagcaaaatc accaacgttg cggctggtga tctctccaca
     1081 accagtaccg atgctgttaa cggttcccag ttaaacgcaa ccaatattct ggttacgcaa
     1141 aatagccaaa tgattaacca gcttgccggt aacaccagtg aaacctacat cgaagaaaat
     1201 ggtgcaggta ttaactatgt gcgtaccaat gataccggtt taaccttcac cgatgccagc
     1261 gcagcaggta ttggctctac cgctgtgggg tataacactg ttgccaaagg cgatagcagc
     1321 gtggccatgg gttataactc ttttgccaaa ggcgatagca gcgtggccat cggtcagggc
     1381 agctacagcg gcgttgatac gggtatcgct ctgggtagca gctccgtttc cagccgtgta
     1441 atagttaaag gttctcgtaa caccagcgta tcggaagaag gtgttgtgat tggttatgac
     1501 accacggatg gcgaactgct tggtgcattg tcgatcggtg atgatggtaa atatcgtcaa
     1561 atcatcaacg tcgcggatgg ttctgaagcc catgacgctg ttacggttcg ccagttgcag
     1621 aacgccattg gtgcagtcgc aaccacacca accaaatact atcacgccaa ctcaacggct
     1681 gaagactcac tggcagtcgg tgaagactcg ctggcaatgg gcgcgaaaac catcgttaat
     1741 ggtaatgcgg gtattggtat cggcctgaac actttagttc tggctgatgc gatcaacggt
     1801 attgctatcg gttctaacgc acgcgcaaat catgccgaca gcattgctat gggtaatggt
     1861 tctcagacta cccgtggtgc gcagaccaac tacactgcct acaacatgga cgcaccgcag
     1921 aactctgtgg gtgagttctc tgtcggcagt gaagacggtc aacgtcagat caccaacgtc
     1981 gcagcaggtt cggcggatac cgatgcggtt aacgtgggtc agttgaaagt aacggacgcg
     2041 caggtttccc agaataccca gagcattact aacctgaaca ctcaggtcac taatctggat
     2101 actcgcgtga ccaatatcga aaatggcatt ggcgatatcg taaccaccgg tagtactaag
     2161 tacttcaaga ccaacaccga tggcgcagac gccaacgcgc agggtaaaga cagtgttgcg
     2221 attggttctg gctccattgc tgccgctgat aacagcgtcg cgctgggtac cggttccgtg
     2281 gccaacgaag aaaatacgat ctctgtgggt tcttctacca accagcgccg tatcaccaac
     2341 gttgctgccg gtgttaatgc caccgatgcg gttaacgttt cgcaactgaa gtcttctgaa
     2401 gcaggcggcg ttcgctacga caccaaagct gatggctcta tcgactacag caacatcact
     2461 ctcggtggcg gcaatagcgg tacgactcgc atcagcaacg tttctgctgg cgtgaacaac
     2521 aacgacgcag tgaactatgc gcagttgaag caaagtgtgc aggaaacgaa gcaatacacc
     2581 gatcagcgca tggttgagat ggataacaaa ctgtccaaaa ctgaaagcaa gctgagtggt
     2641 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttacacgcc gggagccagc
     2701 atggcctcta ttggtggtgg tacttacaac ggtgaatcgg cagttgcttt aggtgtgtcg
     2761 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
     2821 gaatactccg ccgcgctcgg tgccggtatt cagtggtaat gatccattaa caaatgggta
     2881 cgccacattg cctgatgcgc tacgcttatc aggcctacgg ggtgattcag cactaccctg
     2941 taggccggat aaggcgttca cgccgcatcc ggcaagataa ggcgcaccag atcaacaatc
     3001 tgagggcaat tctctgatgg gaattgccct tttcttcatt acctctctcc caggattggc
     3061 cctaccaatt cttcattgat ctgacctctg gttcacagtt ccccaattaa aactcacatc
     3121 accgttgcca atacataaca tttggttaac tatccattgt cattatccct acacaacaca
     3181 attggcagtg ccacttttac acagcgtgtg acaaggagat gagcaacaga ctcattacac
     3241 gatgtgggtg gactccagga gacctgcaat gaatctctgg caacaaaact acgatcccgc
     3301 cgggaatatc tggctttcca gcctgatagc atcgcttccc atcctgtttt tcttctttgc
     3361 gctgattaag ctcaaactga aaggatacgt cgccgcctca tggacggtgg caatcgccct
     3421 tgccgtggct ttgctgttct ataaaatgcc ggtcgctaac gcgctggcct cggtggttta
     3481 tggcttcttc tacgggttgt ggcccatcgc gtggatcatt attgcagcgg tgttcgtcta
     3541 taagatctcg gtgaaaaccg ggcagtttga catcatccgc tcgtctattc tttcgataac
     3601 ccctgaccag cgcctgcaaa tgctgatcgt cggtttctgt ttcggcgctt tccttgaagg
     3661 agccgcaggc tttggcgcac cggtagcaat taccgccgca ttgctggtcg gtctgggctt
     3721 taaaccactg tacgccgccg ggctgtgcct gattgttaac accgcgccag tggcatttgg
     3781 tgcgatgggt attccgattc tggtcgccgg gcaggtaaca ggcatcgaca gctttgagat
     3841 tggtcagatg gtggggcgtc agctgccgtt tatgaccatt atcgtgctgt tctggatcat
     3901 ggcgattatg gacggttggc gcggtatcaa agagacgtgg cctgcggtcg tggttgcggg
     3961 cgggtcgttt gccattgctc aatacctcag ctctaacttt attgggccgg aactgccaga
     4021 cattatctct tcgctggtat cgctgctctg tctgacactg ttcctcaaac gctggcaacc
     4081 agtgcgcgta ttccgcttcg gtgacttagg ggcgtcacag gttgatatga cgctggctca
     4141 cacaggttac acggcaggtc aggtgctgcg cgcctggaca ccgttcctgt tcctgaccgc
     4201 caccgtaacg ctgtggagta tcccgccgtt taaagccctg ttcgcttcag gcggtgcgct
     4261 gtatgagtgg gtgatcaaca ttccggtgcc gtacctcgat aaactggttg cccgtatgcc
     4321 gccagtggtc agcgaagcga cagcgtatgc cgccgtgttt aagtttgact ggttctctgc
     4381 caccggtacc gccattctgt ttgctgcctt gctctcgatt gtctggctga agatgaaacc
     4441 atctgacgct atcagcacct tcggcagcac gctgaaagaa ctggctctgc ctatctactc
     4501 catcggcatg gtgctggcgt tcgcctttat ctcgaactat tccggtttgt catcaacact
     4561 ggcgctggca ctggcgcaca ccggtcatgc attcaccttc ttctcgccgt tcctcggctg
     4621 gcttggggta ttcctgaccg ggtcggatac gtcatctaat gctttgtttg ccgctttgca
     4681 ggctaccgcc gcacaacaaa ttggcgtttc tgacctgttg ctggttgcag ccaacaccac
     4741 cggtggtgtc accggtaaga tgatctcccc gcaatctatc gctatcgcct gtgcggcggt
     4801 aggcctggtg ggcaaagagt ctgatttgtt ccgctttact gtcaaacaca gcctgatctt
     4861 cacctgtatg gtgggcgtga tcaccacgct tcaggcctat gtcttaacgt ggatgattcc
     4921 ttaatgattg ttttacccag acgcctgtca gacgaggttg ccgatcgtgt gcgggcgctg
     4981 attgatgaaa aaaacctgga agcgggcatg aagttgcccg ctgaacgtca actggcgatg
     5041 caactcggcg tgtcgagaaa ttcactgcgc gaggcgctgg cgaaactggt aagcgaaggt
     5101 gtgctgctca gtcgacgtgg cggcgggacg tttattcgct ggcgtcatga cacatggtcg
     5161 gagcaaaaca tcgtccagcc gctgaaaacg ctgatggccg atgatccgga ttacagtttc
     5221 gatattctgg aagcccgata cgccattgaa gccagcaccg catggcatgc agcaatgcgc
     5281 gccacacctg gcgaaaaaga aaagattcag ctttgctttg aagcaacgct aagtgaagac
     5341 ccggatctcg cctcacaagc ggacgttcgt tttcatctgg cgattgccga agcctcacat
     5401 aacatcgtgc tgctgcaaac catgcgcggt ttctttgatg tcctgcaatc ctctgtgaag
     5461 catagccgcc agcggatgta cctggtaccc ccggtttttt cacagctgac caaacaacat
     5521 caggcggtca ttgacgccat ttttgccggt gatgctgacg gggcgcgtaa agcaatgatg
     5581 gcgcacctta gctttgttca caccaccatg aaacgattcg atgaagatca ggctcgccat
     5641 gcacgaatta cccgcctgcc tggtgaccat aatgagcatt cgagggagaa aaacgcatga
     5701 ttatttccgc agccagcgat tatcgcgccg cagcgcaacg cattctgccg ccgttcctgt
     5761 tccactatat ggatgggggg gcatattctg aatacacgct gcgccgcaac gtggaagatt
     5821 tgtcagaagt ggcgctgcgc cagcgtattc tgaaaaacat gtctgactta agcctggaaa
     5881 cgacgctgtt taatgagaaa ttgtcgatgc cggtggcgct aggtccggta ggtttgtgtg
     5941 gcatgtatgc gcgacgcggc gaagttcagg ctgccaaagc cgcggatgcg catggcattc
     6001 cgtttactct ctcgacggtt tccgtttgcc cgattgaaga agtggctccg gctatcaaac
     6061 gtccgatgtg gttccagctt tatgtgctgc gcgatcgcgg ctttatgcgt aacgccctgg
     6121 agcgagcaaa agccgcgggt tgttcgacgc tggttttcac cgtggatatg ccaacgccgg
     6181 gagcgcgtta tcgtgatgcg cattctggga tgagcggccc gaacgcggca atgcgccgct
     6241 acttgcaggc ggtgacgcat ccgcaatggg cgtgggatgt gggcctgaac ggtcgtccgc
     6301 atgatttagg taatatctcg gcttacctcg gcaaaccaac cggactggaa gattacatcg
     6361 gctggctggg gaataacttc gatccgtcca tctcatggaa agaccttgag tggatccgcg
     6421 atttctggga tggcccgatg gtgatcaaag ggattctcga tccggaagat gcgcgtgatg
     6481 cagtacgttt tggtgctgat ggaattgtgg tttctaacca cggtggccgc cagctggacg
     6541 gtgtgctgtc ttctgcccgt gcgttgcctg ctattgcaga tgcggtgaaa ggtgatatcg
     6601 ccattctggc ggatagcgga atacgtaacg ggcttgatgt cgtgcgtatg attgcgctcg
     6661 gtgccgacac cgtactgctg ggtcgtgctt tcctgtatgc actggcaaca gcgggccagg
     6721 cgggcgtggc aaatctgctt aatctgatcg aaaaagagat gaaagtggca atgacgctga
     6781 ctggcgcgaa atcgattagc gaaattacgc aagattcgct ggtgcagggg ctgggtaaag
     6841 agttgccggc ggcattggct ccgatggcga aagggaatgc agcttaaggg ttagacgaat
     6901 atctgctatc ctgcccccgc actaaggggg cagtatgcta aacatcgtac tttacgaacc
     6961 agaaattccg ccaaatactg gcaacatcat ccgtctttgc gctaataccg gctttcgtct
     7021 gcatatcatc gaaccgatgg gatttgcctg ggacgataag cgcctgcgcc gcgcggggct
     7081 ggactatcac gagtttaccg ccgttacgcg tcatcatgac tatcgcgcgt ttctcgaagc
     7141 agaaaatccc cagcgtctgt ttgccctcac cacgaaaggt acgcctgctc acagcgccgt
     7201 aagctatcag gatggcgatt atctgatgtt cggcccggaa acacgcggcc tgccagcgag
     7261 cattcttgat gccctgcccg ctgaacaaaa aattcgtatt ccaatggtgc cggacagccg
     7321 cagcatgaac ctgtccaatg cggtgtcggt cgtggtgtat gaagcctggc ggcagttggg
     7381 gtatccggga gcggtgttga gagattagtt actgtaggcc ggatagatga ttacatcgca
     7441 tccggcacga gcacaggaca tcagatccca tcaccatact caaacgtatg gttaatgccg
     7501 ttgaaatgct gatccatatc cattgacggt ttatcgctat ccggcttgcc gacaatgcgt
     7561 gccggaacgc cagcggcagt agtgtgcggc ggcaccggtt gcaacaccac ggaacctgcg
     7621 ccaatcttcg cgccgcgccc aacttcaata ttaccgagaa ttttcgcgcc cgcgccaatc
     7681 atcacgcctt cacgaatttt cgggtgacgg tcaccaccag atttacccgt accgccaagc
     7741 gtcacagatt gcagaatcga tacgtcgttt tcaatcaccg ccgtttcacc aacgacgatg
     7801 cctgtcgcgt ggtcaagcat gataccgcga ccaatttttg ctgccgggtg aatatcaacc
     7861 tggaacgtta cagagacctg gttttgtaga aaaatagcca gcgcgcgacg gccctgattc
     7921 cacaaccagt gaccgatgcg atacgcctgc aaagcatgaa aacccttcag atatagcaac
     7981 ggggttgagt atttatccac cgccgggtca cgggtacgca ccgcctgaat atcacaggcc
     8041 gcagaggcga tcatttccgg gtcggcggcg taggcttctt ccaccacttc acggatagca
     8101 atagccggca taatgggcga tgacagcttg ttcgccagca tgtagctcag tgcactgcca
     8161 aggttttcgt gcttgagtag cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc
     8221 gccagcgttc tggcttcagc tttaatattg ttccagacaa tttccagttc ttcacacgac
     8281 attgcttact ccacacgatg agataatgac cggcccgttc tgcgcgggcc gggtcatagt
     8341 ggtaacaaag gttccctggg tttagtggct gctgcgctcg tccttgcgtg cacgacctag
     8401 taaagtcaat gctgcctcgc gcgcgttttt tccgcaatat aatacttgat aaatttcctc
     8461 ggttattggc atttcaacgc cgaagcgatg cgccagttcg cggacttctt tcgtattgcg
     8521 gtagccttcc accacctgac ctatcttttc ctgcgcgctt tgtacatcca tgccctgacc
     8581 gagcatcatg ccaaaacggc ggttacgcga ctggttatca gtacaggtaa gcaccagatc
     8641 gcccagcccc gccatgccca taaaggtggc aggatcggca cccagcgccg cgccaagacg
     8701 cgacatttca gccagtccac gggtgatcag cgccgtacgc gcattcgcgc caaaaccgat
     8761 accgtcggac atccccgcgc caatggcaat gacgtttttc accgcaccgc caagctgcac
     8821 accgatgaaa tccgggttgc tgtaaacgcg gaaacttttg ccacagtgca gcaattgctg
     8881 gaggtcatcg gcaaaggtct ggtccgtcga ggccagcgaa atggccgtcg gtaaacctgc
     8941 cgccagttct ttcgcaaacg ttgggccaga gataaccgcc agcggaattt gatcgcctaa
     9001 ggcctcacgc gccacgtcct gtaacagacg tccggtttct gcttccagcc ctttggtcgc
     9061 ccacaccaga cgcgcatcag gacgcatcag cggtttaatc tggcgcagca cttcaccaaa
     9121 gacatggctg ggtacgacga cgagaatatt acggctggct gccagcgcag tggcgagatc
     9181 gctttcaaga tggagcgtat cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg
     9241 gtcgcgttca agcgttgcga tatgttcagg gtcatggccc cagaggacaa cctcgtggcc
     9301 atttcttgcc agggtgatgg caagagcggt gccgtacgag ccggcaccga tcacagtcat
     9361 tgaagcatta cgttggttca tcaggcatcc tgatgttctt cagtaccttc gccagcctgc
     9421 tgctgcaaat agttcatgaa cagcgcatcg aagttaaccg gtgcaaggtt cagttgcggg
     9481 aatgtaccgc gggataccat gctggtgatg cactcacgag catacgggaa cagaatgttc
     9541 gggcagtatg cgcccaggca atgtgccatc tgggtgcctt cgatacccgc gatggagaaa
     9601 ataccgccct gctgaacttc acacaggaac gcggtttctt cgcccaggga ggccgttacg
     9661 gtaacacgca gtaccacttc gtaaacgtca tctgccagtt gagtagatgc cgtatccaga
     9721 tcaagtttaa cttctggttg ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg
     9781 aaagaaatat ccttggtata aatacgttgg atctggaaag tcatttcggt gttgttttgt
     9841 tctgacatgt gtagaaaacc cttaagtgtt gtccttaaaa tactgcgcaa tgtaatgcca
     9901 acgttcacac gttatttcag caggggatcc agtccaccac gtgcatccaa tgcatacaag
     9961 tcatcacagc caccaatgtg ctgtgcgtca ataaaaatct gtggaaccgt ggtgcgaccg
    10021 ctgcgtttga tcatctcttc acgcttagcg gcattgccat cgatcggcaa ctcctgaaaa
    10081 ctcacgccct tgctgctcag cagtgctttt gcacgatggc aatacgggca ggtttctttg
    10141 gtatagattt caacattggc cataaattag ctcctgtttt ttaccctgaa atttcatgtt
    10201 gcagggcggc agcaaaatgg agtgtcccca gaagcttata acaataagtc gctgggggcc
    10261 taattgcagc taacggcctg catcatgaaa aacggcaggt aaattatttg ccgcgcacca
    10321 aaggcaagtt ttcgccagcc cagccagcga cgccttcttt cagtacgaat acttgcgcaa
    10381 aaccagcttt cgtcagcgcg tttgcaggct cctggcactg catgccagaa ccatctacca
    10441 cgataaccgg tttgtctttg tgcttctcaa gctcaccaac attgttggct ttgatttcgc
    10501 tcggcaacag gttgatagaa cctgcgatat ggcctttacg gaagtcatca cgctgacgta
    10561 aatccacaac cacagcgtct tctttgttga tcagacgcgt agcttcacca cgagtaatca
    10621 ccttcacttt cgaggtcagg ctcttaaacg tagtcacaag aaccgccacc agtaacgcga
    10681 tccaggcgat actcagtatg ggatgacggc caacaaattg cataatttct tgcatggggg
    10741 gtaacaactc ccgacgtagt gattaaaaaa ccaggaaagg agtatacctg cgcgttgcgg
    10801 caaatacagc cagcgcgtta actggaatgc aattttgcgg ggcgctacga aaaaaaagcg
    10861 taaatctggc atctgcttgc cgcgagcggt cgtatatttt gatcttcaga ggctatttta
    10921 tcgattcagc tgtagtaaaa ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat
    10981 gtcggtttct aaaaaaccta tggtactggt gattctggat ggctatggct atcgcgaaga
    11041 acagcaggat aacgccattt ttagtgctaa aaccccggta atggatgcac tgtgggccaa
    11101 tcgtccgcat accctgatcg acgcttccgg tctggaagtc ggtctgcctg accgtcagat
    11161 gggtaactcc gaagtaggcc acgttaacct gggtgccggc cgcatcgtgt atcaggacct
    11221 gactcgtctg gacgttgaaa tcaaagatcg cactttcttt gctaatccgg tgttgactgg
    11281 tgcggtagat aaagcgaaaa acgcaggcaa agcagtacac attatgggtc tgctctccgc
    11341 aggtggtgta cacagccacg aagatcacat catggcgatg gtagaactgg cagctgaacg
    11401 cggcgcagaa aaaatctacc tacacgcatt ccttgacggt cgcgacactc cgccgcgcag
    11461 tgctgaatcc tcgctgaaaa aattcgaaga gaaatttgcc gcgctgggta aaggccgcgt
    11521 agcgtccatc attggtcgtt actacgcgat ggaccgcgat aaccgttggg atcgcgttga
    11581 aaaagcttat gacctgctga ctctggcgca aggtgagttc caggccgata ccgccgttgc
    11641 cggtttgcag gctgcttatg ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat
    11701 tcgtgcagaa ggtcagccag atgcggcaat ggaagacggc gatgcgctga ttttcatgaa
    11761 cttccgtgct gaccgcgcac gtgaaatcac tcgtgcattc gtaaatgccg attttgacgg
    11821 cttcgcgcgt aagaaagtgg ttaacgtcga tttcgtgatg ctgaccgaat acgctgccga
    11881 catcaaaacc gcagtggctt acccacccgc ttcccaggtt aacaccttcg gcgagtggat
    11941 ggcgaaaaac gacaaaactc agctgcgtat ttccgaaacc gaaaaatatg cccacgttac
    12001 tttcttcttc aacggtggcg tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa
    12061 ctcaccgaaa gtggctacct acgatctgca accggaaatg agctccgcag agctgaccga
    12121 aaaactggtt gcggcaatca agagcggcaa atacgacacc attatctgta actatccgaa
    12181 cggcgacatg gtcggtcaca ccggagtgat ggaagcggcg gttaaagcgg ttgaagcgct
    12241 ggatcactgc gtggaagaag tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat
    12301 caccgctgac cacggtaacg ctgaacagat gcgcgatccg gcaacgggtc aggcacacac
    12361 agcacacacc aacctgccag ttccgctgat ttacgttggt gataagaacg tgaaagcggt
    12421 tgaaggcggc aaactttctg acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat
    12481 cccgcaagag atgactggta agccgctgtt catcgtggaa taatccctcc ccatgagggg
    12541 aaaggcgatt aataccatga cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat
    12601 catctacgcc agcgttctga gcgctggcgt attgttgtgc gccttttccg cccacgcgga
    12661 tgagcgtgac caactcaaat ccattcaggc tgacatcgcc gcgaaagagc gcgcggtacg
    12721 ccaaaagcaa caacaacgcg caagcctgct cgcacaattg aaaaagcagg aagaagcgat
    12781 ctctgaagcc acccgtaaac tgcgcgaaac gcaaaatacg cttaatcaac tgaataaaca
    12841 gattgatgag atgaacgcgt cgattgccaa actagagcag caaaaagccg cccaggagcg
    12901 cagcctcgcc gcgcaactgg atgccgcgtt tcgtcagggt gaacataccg gtattcagct
    12961 gattctcagc ggtgaagaaa gccagcgtgg gcagcggtta caggcttatt tcggctatct
    13021 caaccaggcg cgacaagaaa ccattgctca gttgaaacaa acgcgtgaag aagtcgctat
    13081 gcagcgtgcc gaactggaag agaaacaaag cgagcaacaa acgcttttat atgagcagcg
    13141 cgcccaacag gcgaagctga cccaggcgtt gagcgagcgt aaaaagacgc tggcagggct
    13201 ggagtcttcc atccagcaag gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg
    13261 cctgcgtaac agcattgccc gtgcggaagc cgcggcgaaa gcgcgtgctg aacgtgaagc
    13321 gcgcgaagcc caggcggttc gcgaccgcca gaaagaagcg acgcgcaaag gcaccaccta
    13381 caagccgacc gaaagcgaaa aatcgctgat gtcccgtacc ggtggtctgg gcgcaccgcg
    13441 cggtcaggca ttctggccag ttcgcgggcc aacgctgcat cgctatggcg aacagctaca
    13501 gggtgaatta cgctggaaag ggatggtgat tggtgcctct gaaggtactg aagttaaagc
    13561 gattgccgac ggccgggtga ttctggctga ctggctgcaa ggctacggtc tggtggtggt
    13621 ggttgagcat ggtaaaggcg acatgagtct ttacggctat aatcagagcg cactggtgag
    13681 cgttggttcg caggttcgcg cgggccagcc aattgcactg gtgggcagca gtggcggtca
    13741 gggtcggcct tcactctatt tcgaaattcg ccgccagggt caggcggtca atccacagcc
    13801 gtggttggga agataagttt tgtttccatt tcgtcgtaac gttcttgcat ttgccgccct
    13861 gttggcgctc tcctcccccg taattgctgg caaacttgcc atcgtcattg atgattttgg
    13921 gtatcgcccg cacaacgaaa accaggtgct ggcgatgcct tccgctatct ccgtcgctgt
    13981 attacccgat tcaccgcacg ccagagagat ggcgaccaaa gcacataaca gcggtcacga
    14041 agtgttgatt catctaccga tggcaccact gagtaaacag ccgctggaga aaaatacgct
    14101 acgcccggag atgagcagcg acgaaattga gcgcattatt cgtagtgcgg tcaataacgt
    14161 gccctatgcc gtggggatca acaaccacat gggcagcaag atgacctcta acctgtttgg
    14221 tatgcagaaa gtgatgcagg cgctggagcg ttacaatctt tacttcctcg acagcgtaac
    14281 catcggtaat acccaggcga tgcgcgccgc gcaaggcact ggcgtgaagg tgatcaaacg
    14341 gaaggtgttc ctcgacgatt cgcaaaatga agcggacatc cgtgtgcaat tcaatcgcgc
    14401 aattgacctg gcgcgtcgca acggttcaac cattgccatt ggacatcctc acccttcaac
    14461 ggtacgcgtg ctacaacaga tggtttataa cctgccgcca gacattacgc tggtgaaagc
    14521 cagcagcttg ttgaatgaac cgcaggttga tacgtctaca ccgccgaaaa acactgtgcc
    14581 cgacacaccg cgtaatccat tccgtggcgt gaagctgtgc aaaccgaaga agccgctgga
    14641 acctgtttat gctaatcgct tctttgaagt attaagcgaa agcatcagcc agagcacgct
    14701 gatcgtttac ttccagcatc agtggcaagg ctggggcaaa cagcccgaag cggcaaaact
    14761 taacgctagc gcaaattaaa ggcgctacgc gcaatgcgat ggtgcgaccg cgttttgtcg
    14821 cgccattgcc ataagcggaa agaccacagt aacgcctggt aacccacctt cacgctgcgg
    14881 acattggtaa tcaggcgctt atacataccg gaagtaaata tctcggcaat catccgttgg
    14941 cgggtaataa tatccggctc tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt
    15001 atttgctgat gaaattccgg ataaatcgta attttgtcgg cataatttcg atttaatttc
    15061 tccagcaggc gggtaatctt aatatagtga cgttgataat taaggttttt attcccttgt
    15121 ctatgcaacc gactcactga cgtattatgc agataatatt tatataatga ttgctcggta
    15181 tatcgcgcac gcagcgcgtt aaacataaat tctgttgtcc agacaatatc ctgatgatgt
    15241 aatccggcaa taaatttaat gttattttta acaataacat cgcggcgata aacccccatc
    15301 cagaccacgt gtgtccaacg gcgggaagaa agtcccatcc gcagccagtc cggaccagtt
    15361 aatacaccgg ttgagcgaag gcgatcggta gggatggatt gccaggtttc acccgtttca
    15421 cgaaaacacc aatcagcgtt gcactgcgcc acgtcgaggt cgtcctctaa cgccatggtc
    15481 atcagcgttt cgtacatggt gggatagacc tcatcgtcag catcgacaaa agcgacatat
    15541 tttcccgttg ccacttcaat cccacgatta cgcgccaccg atgcgccagc attcgcctga
    15601 tgcaacaaac gaacgtgcgg atagttttct gcgtaatgct ttgctatttc aacagaatta
    15661 tccgttgaac catcgttaat aataatgatt tccagagcag tccatgtttg cgtaattaaa
    15721 gattccatac aagtgcggaa atcatcgccc gcattatata acggaataat aacactaagt
    15781 ttattggtgc tgttcatcat aaattaccga cgacctctga agttatcctg acgttttaca
    15841 ggctttgtat taagaaacta ttaagcctgt gagatattat ttttaccggt gagagagtca
    15901 cgtcaggata gagagataat gagagcagct atataagaga tcagtgcaat tacgaactct
    15961 tgattacact gacaggattt tacatattta acggattatc ctcaggcaaa agggctggaa
    16021 taccagccct tgttcgtgtt aatcccagct cagaataact ttcccggact ggcccgaacg
    16081 catggcatca aagcccttct ggaaatcatc gatagagaaa cgatgggtaa tgatcggcga
    16141 gagatcgagg ccagactgaa tcagcgccgc catcttgtac caggtttcaa acatctcacg
    16201 accgtaaata cctttaatga acaagccttt aaagattact ttggtccagt cgatagacat
    16261 atcggacggt ggaataccca gcatcgcaat acgaccgccg tggttcatgg tgtcgagcat
    16321 ggtacgaaac gctggcggcg caccggacat ttccagaccg acatcaaagc cttcggtcat
    16381 gcccagttct gccatcacat cattaaggtt ttctttagcg acgttaaccg cacgggtgat
    16441 acccattttg cgtgccagct caaggcggta ttcgttaaca tcagtgatca ccacattgcg
    16501 tgcaccaacg tgtttcgcca ccgccgcagc cataatacct atcggacctg caccagagac
    16561 cagcacatcc tcgccaacca gatcgaacga cagcgcggta tgcacagcgt taccgaaggg
    16621 atcaaaaatg gaagccaggt cgtcggagat attgtccggg attttgaagg cattgaacgc
    16681 cggaatcacc agatactcgg cgaagcagcc cggacggtta acaccgacgc cgatcgtgtt
    16741 gcggcacaga tgggtacgac cgccacgaca gttgcgacag tgaccgcagg tgatatggcc
    16801 ttcgccagaa acgcgatcgc caatcttgaa gcctttcact tcctgaccaa tacctaccac
    16861 ttcaccgaca tattcatggc ccacgaccat cggcaccggg atggtttttt gcgaccactc
    16921 atcccagtta tagatgtgaa cgtcagtccc gcagatggct gttttacgga ttttaatcag
    16981 cagatcgtta tgcccgagtt ccggtacagg aacgtcggtc atccagatgc cctcttccgc
    17041 tttcagtttg gataacgctt tcatctcaca ccctcaggcg ataacgccca gttgtttacc
    17101 aatacgcgtg aatgcttcta ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat
    17161 ctgggtacga atacgcgcct gacctttagg aacgaccgga tagaagaaac cggtgacgta
    17221 aatgccctct ttttgcagct cacgggcaaa tttctgcgcc actaccgcat caccaagcat
    17281 gaccggaata atggcgtgat cggctcccgc cagggtaaag cccgccgccg acatttgctc
    17341 acggaactga cgcgcgttcg cccacagacg gtcacgcagc tcgctacccg cttcgaccat
    17401 ctccagcact ttgatggatg cggcgacaat cgctggtgcc agcgagttgg agaacaggta
    17461 cggacgagaa cgctggcgca gccactcaac cacttctttg cgcgccgcgg tataaccacc
    17521 agaagccccg cccaacgctt tacccaacgt accggtaatg atgtctacac gtcccatcac
    17581 atcgcagtat tcatgggaac cacgaccatt ttcaccgaca aaaccgaccg cgtgggagtc
    17641 gtctaccatc accagggcat cgtatttatc tgccagatcg caaacgccct tcaggttggc
    17701 aatcacaccg tccatcgaga acacaccatc ggtggcaatc agcacatgac gcgcaccggc
    17761 ttcacgcgct tctttcagac gtgcttccag ctcctgcata tcgttgttgg cataacgata
    17821 gcgtttggct ttacacagac gcacaccatc aataatagac gcgtggttca gtgcgtcgga
    17881 gataatggcg tcctccgcgc tcagcagcgt ttcaaacagg ccgccgttag catcaaagca
    17941 ggaagagtag agaatcgcat cttccatccc cagaaacgcc gccagttttt gttcaagctc
    18001 tttatggctg tcctgagtgc cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga
    18061 atccattccc gcctttgccg ccgcaatcag atccggatga ttcgccagtc cgagatagtt
    18121 gttggcacaa aagttaatga cgtggcttcc atcagccaca gtgatatctg cttgctgcgc
    18181 agacgtaata atgcgctctt ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt
    18241 ggttaactgc tgataaaatt ctccacgcat tgcgattctc cagacagggc aaattccagc
    18301 acatattacc caaacttata ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc
    18361 aggataaata acgtccagcc ccctcacttt tggtgaatgg ctgaagccaa cgtcatgtag
    18421 tgatatgata gaagtattcg tgtctgagat tgtctctgac tccataattc gaaggttaca
    18481 gttatgatca tcgttaccgg cggcgcgggc tttatcggaa gcaacatcgt taaagccctg
    18541 aatgataaag gcatcaccga tattctggtg gtggacaacc tgaaagacgg caccaagttt
    18601 gtgaacctgg tggatctgga tatcgcggac tatatggata aggaagactt cctgatccag
    18661 attatggctg gcgaagagtt cggcgatgtc gaagcgattt tccacgaagg cgcgtgctct
    18721 tccaccaccg agtgggacgg caagtatatg atggataaca actatcaata ctccaaagag
    18781 ctgctgcact actgcctgga gcgcgaaatc ccgttcctgt acgcttcttc tgcagccact
    18841 tacggcggac gcacttccga ctttattgaa tcccgcgagt acgaaaaacc acttaacgtc
    18901 tacggttatt cgaagttcct gtttgatgaa tatgttcgcc aaatcctgcc ggaagccaac
    18961 tcgcagattg ttggcttccg ctatttcaac gtttatggac cgcgtgaagg ccataaaggc
    19021 agcatggcga gcgtcgcttt ccatctcaac acccagctta ataacggtga atcaccgaag
    19081 ctgttcgaag gtagcgagaa ctttaaacgt gacttcgtct atgtgggcga cgtggcagat
    19141 gtaaacctgt ggttcctgga aaatggcgtt tccggcatct tcaacctcgg caccggtcgt
    19201 gcggaatcct tccaggcagt agcagatgct acgcttgctt atcacaagaa aggccaaatc
    19261 gaatacattc cgttcccgga taaactgaaa ggccgctacc aggcgttcac tcaggcagat
    19321 ctgacgaatc tgcgcgcggc gggttacgat aaaccgttca aaaccgttgc cgaaggcgta
    19381 acggaataca tggcctggct gaatcgtgac gcataagagc tctgcatgaa aatactggtg
    19441 atcggcccgt cttgggttgg cgacatgatg atgtcgcaaa gtctctatcg cacgctccag
    19501 gcgcgctatc cccaggcgat aatcgacgtg atggcaccgg catggtgccg tccattatta
    19561 tcgcggatgc cggaagttaa cgaagcgatc cctatgcctc tcggtcacgg agcgctggaa
    19621 atcggcgaac gccgcaaact gggtcatagt ctgcgtgaaa agcgctacga ccgcgcctac
    19681 gtcttaccta actccttcaa atccgcatta gtgcctttct tcgcaggtat tcctcatcgc
    19741 accggctggc gcggcgagat gcgctacggt ttactcaacg atgtacgcgt gctcgataaa
    19801 gaagcctggc cgctaatggt ggaacgctat gtcgcgctgg cctatgacaa aggtattatg
    19861 cgtaccgcac aagatctgcc gcagccattg ttatggccgc agttgcaggt gagcgaaggt
    19921 gaaaaatcat atacctgtaa tcaattttcg ctttcatcag aacgtccgat gattggtttt
    19981 tgcccgggtg cggagtttgg tccggcaaaa cgctggccac actaccacta tgcggagctg
    20041 gcaaagcagc tgattgatga aggttatcag gtggttctgt ttggctcggc gaaagatcat
    20101 gaagcgggca atcagattct tgccgctttg aatacagagc agcaggcatg gtgtcggaat
    20161 ctggcgggag aaacacagct tgatcaagcg gttatcctga ttgcagcctg taaagccatt
    20221 gttactaacg attctggtct gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc
    20281 ctgtatggac caagcagccc ggacttcaca ccaccgctat cccataaagc acgcgtgatc
    20341 cgcctgatta ccggctatca caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag
    20401 agcttgatcg acattactcc ccagcgcgta ctggaagaac tcaatgcgct attgttacaa
    20461 gaggaagcct gacggatgcg ggttctgatc gttaaaacat cgtcgatggg cgatgttctc
    20521 cacacgctgc ccgcactcac tgatgcccag caggcaatcc cagggattaa gtttgactgg
    20581 gtggtggaag aagggttcgc acagattcct tcctggcacg ctgccgttga gcgagttatt
    20641 cctgtggcaa tacgtcgctg gcgtaaagcc tggttctcgg cccccataaa agctgaacgc
    20701 aaagcgtttc gtgaagcgct acaagcaaag aactatgacg cagttatcga cgcccagggg
    20761 ctggtaaaaa gcgcggcgct ggtgacgcgt ctggcgcatg gcgtaaagca tggaatggac
    20821 tggcaaaccg ctcgcgaacc gttagccagc ctgttttaca atcgtaaaca tcatattgca
    20881 aaacagcagc acgccgtaga acgcacccgc gaactgtttg ccaaaagtct gggctatagc
    20941 aaaccacaaa cccagggcga ttatgctatc gcacagcatt tcctgacgaa cctgcctaca
    21001 gatgctggcg aatatgccgt atttcttcat gcaacaaccc gagatgataa acactggccg
    21061 gaagaacact ggcgagaatt gattggttta ctggctgatt caggaatacg gattaaactt
    21121 ccgtggggcg cgccgcatga ggaagaacgg gcgaaacgac tggcggaagg atttgcttat
    21181 gttgaagtat tgccgaagat gagtctggaa ggcgttgccc gcgtactggc tggggctaaa
    21241 tttgtagtgt cggtggatac ggggttaagc catttaacgg cggcactgga tagacccaat
    21301 atcacggttt atggacctac cgatccggga ttaattggtg ggtatgggaa gaatcagata
    21361 gtttgtaggg ctccggggaa tgagttgtct caattgacag caaatgctgt taagcaattc
    21421 at
//
LOCUS       DADSZD010000076        21412 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.076, whole genome shotgun sequence.
ACCESSION   DADSZD010000076 DADSZD010000000
VERSION     DADSZD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.076"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            16..6123
                     /gene="irp2"
                     /locus_tag="J1Z28_002506"
     CDS             16..6123
                     /gene="irp2"
                     /locus_tag="J1Z28_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HBA7717333.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDTYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     VVRDGQQQVLEQTPRWVIPAHILHTPEEALQVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            6211..15702
                     /gene="irp1"
                     /locus_tag="J1Z28_002507"
     CDS             6211..15702
                     /gene="irp1"
                     /locus_tag="J1Z28_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HBA7717334.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLDALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLSQRWLAARGDTLWLPSLTPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAVVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPAHWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            15699..16799
                     /gene="ybtU"
                     /locus_tag="J1Z28_002508"
     CDS             15699..16799
                     /gene="ybtU"
                     /locus_tag="J1Z28_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HBA7717335.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPQVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            16862..17599
                     /gene="ybtT"
                     /locus_tag="J1Z28_002509"
     CDS             16862..17599
                     /gene="ybtT"
                     /locus_tag="J1Z28_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HBA7717336.1"
                     /translation="MCPFAGGSSSAFRHWQAEQLADCALSLVTWPGRDRLRHLEPLRN
                     ITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFETCRLLEQRGLAPQGLIISGCHA
                     PHLHSERQLSHRDDADFIAELIDIGGCSPELRENQELMSLFLPLLRADFYATESYHYD
                     SPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLSHVTGPVVIDGDHFYPIQQARSF
                     FTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            17603..19180
                     /gene="ybtE"
                     /locus_tag="J1Z28_002510"
     CDS             17603..19180
                     /gene="ybtE"
                     /locus_tag="J1Z28_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HBA7717337.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENLAELARQMAHKHACLRHVLVAGETVSDDFTPLFSLHGER
                     QAWPQPDVSATALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            19311..21332
                     /gene="fyuA"
                     /locus_tag="J1Z28_002511"
     CDS             19311..21332
                     /gene="fyuA"
                     /locus_tag="J1Z28_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HBA7717338.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNIPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
BASE COUNT         4302 a         6569 c         6192 g         4349 t
ORIGIN      
        1 ccatcaggag gaagaatgat ttctggcgca ccatctcagg attcgctgtt accggacaac
       61 cgccacgcgg ctgattacca acaattacgc gagcggctca tacaggaact gaatttaacg
      121 ccgcagcagt tacatgaaga gagcaacctg atccaggccg gcctggattc cataagattg
      181 atgagatggt tacactggtt tcgtaaaaat ggctaccgcc ttacccttcg cgagctgtat
      241 gccgccccca cgctggcggc atggaaccag ttaatgctca gccggtcgcc ggagaacgcg
      301 gaagaagaaa cgccgcccga cgaatcatcc tggccgaaca tgaccgaaag tacccccttc
      361 ccattgacgc cagtacagca cgcctacctg acgggccgca tgccggggca gacgcttggc
      421 ggcgtgggtt gccacctgta tcaggagttt gaaggccatt gtctgacggc gtcgcagctg
      481 gagcaggcca tcacgacctt gctgcaacgc cacccaatgc tgcatatcgc ctttcgcccc
      541 gacgggcagc aggtctggct accgcaacct tactggaacg gcgtcaccgt tcatgattta
      601 cgccataacg acgctgaaag ccgccaggcc tatctggacg cactgcgcca gcgcctgagc
      661 caccgtcttt tacgcgtgga aatcggcgaa acgtttgatt ttcagctgac gctcttgccg
      721 gacaatcgcc accgcctcca tgtcaatatt gacctgctga ttatggatgc ctccagcttt
      781 acgcttttct tcgatgagct taacgccctg ctggccggag aatcgctgcc ggctatcgac
      841 acccgctatg atttccgctc gtatttgctg caccagcaga agatcaatca accactgaga
      901 gacgacgcgc gcgcttactg gctggcgaaa gcatcgacgc ttccccccgc gcccgtcttg
      961 ccgctggcct gcgaacccgc cacgctacgt gaagtccgta atacccgacg ccgcatgatt
     1021 gtcccggcaa cacgctggca cgcctttagc aaccgggccg gcgagtatgg cgtgacgccg
     1081 acaatggcac tggcgacctg tttttctgcc gtgctggctc gctggggcgg cctgacgcgt
     1141 ctgctgctta acatcacctt attcgaccgc cagccgctgc acccggcggt tggcgcgatg
     1201 cttgccgact tcaccaatat tcttctgctg gataccgcct gcgatggcga taccgtcagc
     1261 aacctggcgc gtaaaaacca gctcacgttt acggaggact gggagcatcg ccactggtcc
     1321 ggcgtcgaat tactccgtga actcaaacgc cagcagcgct acccccacgg cgccccggtg
     1381 gtatttacca gcaatctggg gcgttccctc tacagcagcc gcgcagaatc gccgttgggc
     1441 gagccggaat ggggcatctc gcaaacgccg caggtctgga tagatcatct ggcgttcgag
     1501 catcacggcg aggtctggct acaatgggac agcaacgacg cgctgttccc tccggcgtta
     1561 gtcgaaacat tgttcgacac ctactgccag ttgattaacc aactctgcga tgacgaaagc
     1621 gcctggcaaa agccgttcgc agatatgatg cccgccagcc agcgcgcgat acgcgaacgg
     1681 gtcaacgcca ccggcgcccc cattcccgaa ggcttgctgc atgaaggcat tttccgtatc
     1741 gctctgcaac agccgcaggc gctggcggta acggacatgc gttatcagtg gaattatcat
     1801 gagctgacag actatgcccg ccgttgcgcg ggcaggttaa tcgagtgcgg ggttcagccc
     1861 ggcgataatg tggctatcac gatgtcgaaa ggcgcaggac aacttgttgc ggttctggcc
     1921 gtcctgctgg ctggggcggt ttacgttccg gtttcgctgg atcagcctgc cgcacggcgc
     1981 gagaaaatct acgctgacgc cagcgtccgg ctggtgctca tttgtcagca cgacgccagc
     2041 gccgggtcag acgatattcc cgtccttgcc tggcagcagg ccattgaggc ggagccgatc
     2101 gccaacccgg tagtacgcgc ccccacgcaa ccggcctaca ttatctacac ctccggctct
     2161 accggtacgc cgaaaggggt agtcatttct caccggggag cgcttaacac ctgttgcgat
     2221 atcaataccc gctatcaggt tggcccgcat gacagggtgc tggccctctc cgccctacat
     2281 tttgatttat cggtttacga catttttggc gtactgcgcg cgggcggcgc gctggtgatg
     2341 gtgatggaaa atcaacggcg cgatcctcac gcatggtgtg agctgatcca gcgccatcag
     2401 gtcacgctct ggaacagcgt cccggcgctg ttcgatatgc tgctgacctg gtgtgaaggt
     2461 ttcgccgacg ccacgccgga aaacctgcgc gcagtgatgc tttccggcga ctggatcggg
     2521 cttgacctcc ccgcccgtta tcgggccttc cggccacaag gacaatttat cgcgatgggc
     2581 ggtgccaccg aggcgtctat ctggtctaac gcctgcgaaa ttcacgacgt ccccgcccac
     2641 tggcgctcca tcccttacgg ttttccgcta accaaccaac gctaccgggt ggtggatgaa
     2701 cagggccggg actgccctga ctgggtgccg ggtgaattat ggattggcgg cattggggtc
     2761 gcggaaggct atttcaacga tcccctgcgt agcgagcagc aatttttgac gctcccggac
     2821 gagcgctggt atcgcaccgg cgatctcggc tgctactggc cagatggcac aatcgagttc
     2881 ctcggtcgtc gcgacaagca ggtcaaagtc ggaggatatc gcatcgagct gggcgaaatc
     2941 gaaagcgcgc tcagccagct ggcgggggtg aaacaagcaa ccgttctggc gatcggcgaa
     3001 aaagaaaaaa cgctggcggc atacgttgtt cctcagggcg aggctttttg cgttaccgat
     3061 catcggaacc cggcactgcc gcaggcgtgg cacacgcttg cgggaacgtt gccctgttgc
     3121 gccatctcgc cagagatctc cgcagaacag gtagccgatt tccttcagca tcgcctgcta
     3181 aaactgaagc cgggtcacac cgctggcgcc gatcctctcc ccctgatgaa ctcactcgct
     3241 atccagccgc gctggcaggc cgtggtggaa cgctggttag catttctggt gacacaacgg
     3301 cgactgaagc ccgctgctga aggttatcag gtctgcgctg gtgaagaacg cgaggatgag
     3361 cacccgcact tcagcggaca tgatttaacg ttatcgcaaa ttcttcgcgg tgcccgtaac
     3421 gaactgtcgt tactgaacga cgcgcagtgg tcgccggaaa gcctggcctt taaccatccg
     3481 gccagcgccc cgtatattca ggaactggcg acaatttgcc aacagcttgc acagcgctta
     3541 cagcgcccgg tgcgcctgct tgaggtggga acccgcactg gccgcgccgc agaatcgctg
     3601 ttagcacagc tcaacgccgg acagattgag tatgtcgggc ttgagcagag ccaggagatg
     3661 ctgctgagcg cccggcagag gctcgccccc tggcctggcg cccgtctgtc cctctggaat
     3721 gcagacacgc tggcggcgca cgctcactcg gcggacatta tctggcttaa taacgccctg
     3781 catcgtctgc tgccggaaga tcccgggctc cttgcgacat tacaacagct tgccgttccc
     3841 ggcgcgctgc tctacgtgat ggagtttcgc cagttaacgc cgtccgccct actcagcacg
     3901 ctcctgttaa ccaatgggca gccggaggcc ttgctgcata acagcgccga ctgggcggca
     3961 ttatttagcg cggccggctt caactgtcag catggcgatg aggtcgcggg gttacaacgc
     4021 ttcctcgtac aatgtcctga caggcaggtg cgccgcgatc cccgtcaact tcaggccgcc
     4081 ctcgccgggc gtctgccggg gtggatggtg ccgcaacgga tcgtcttcct cgacgcctta
     4141 ccgctgacgg ctaacgggaa aattgactac caggcgctga agcgtcgtca tacccctgaa
     4201 gcggaaaacc cggccgaagc ggatttaccc cagggcgaca ttgaaaaaca ggttgccgcc
     4261 ctctggcagc aactcttatc aactggcaat gtcaccagag aaaccgactt cttccagcaa
     4321 ggcggcgata gcctgctggc gacccgtctg accgggcaac ttcatcaggc aggttatgaa
     4381 gcgcaattaa gcgacctgtt taatcatccc cggctggcgg attttgccgc cacgctgcgg
     4441 aaaaccgacg tcccggtcga acaaccattc gtccactccc ctgaagatcg ctaccagccc
     4501 tttgcgctta ccgacgtgca gcaggcttac ctggtggggc gtcagccggg ctttgccctg
     4561 ggcggcgtcg gctcacattt ctttgttgaa tttgaaattg ccgatctgga tctcacccgg
     4621 ttggagacgg tctggaaccg attaatcgcc cgccacgata tgctacgcgc cgtcgtgcgt
     4681 gatggacagc aacaggtgct cgaacagacg cctcgctggg taatacccgc acacatcctc
     4741 catacgcctg aagaggcgtt gcaggtgcgc gaaaaactgg cccatcaggt actcaacccc
     4801 gaagtctggc cggtattcga tctccaggtc ggttacgttg acgggatgcc cgcccgcctg
     4861 tggctgtgtc tggataacct gttacttgac gggctgagca tgcagattct gttggcggag
     4921 ctggagcacg gctaccgcta cccgcaacag ctgcttccgc cgctgcccgt caccttcagg
     4981 gattatctgc aacaaccctc gctacagtcg cccaatccag attctctggc atggtggcag
     5041 gcgcagcttg atgatattcc tccggcgcct gcgttgccgc tgcgctgctt gcctcaggag
     5101 gttgaaacac cgcgcttcgc ccgcctgaac ggcgcactgg acagcacgcg ctggcatcgg
     5161 ctgaaaaaac gggcggctga cgcccatctc accccgtcgg ccgtactgtt gtcggtgtgg
     5221 tcaacggttc tctctgcatg gagtgcacag cctgagttca cgcttaacct tacgcttttc
     5281 gacaggcgac cgctgcaccc gcaaatcaac cagattctgg gcgatttcac ctcgctgatg
     5341 ctgctgagct ggcatcccgg cgaaagctgg ctgcacagcg cgcagtcact acagcagcgg
     5401 ctgagccaga acctcaacca ccgcgatgtg tcagccatcc gcgtgatgcg tcaactggcg
     5461 caacggcaaa acgtgccagc cgttccgatg cccgtcgtct ttaccagcgc gctgggcttt
     5521 gagcaggata acttcctcgc ccggcgtaat ctgctcaaac cggtctgggg catctcccag
     5581 acgccgcagg tctggctcga tcaccagatt tatgaatccg aaggcgaact gcgctttaac
     5641 tgggattttg tcgccgcgct gtttcctgcc gggcaggtgg agcgccagtt tgaacagtat
     5701 tgcgcattgc taaaccgaat ggccgaggat gaaagcggct ggcaactgcc gctcgccgcg
     5761 ctggtgcctc ccgttaaaca cgcagggcaa tgcgcagagc gctcaccgcg cgtatgccct
     5821 gagcactctc agccacacat tgcggcggac gagagcaccg tcagcctgat ttgcgacgcc
     5881 ttccgcgagg tggttggcga gtctgtcacg cccgcagaaa acttctttga ggcgggcgca
     5941 acgtcgctga atctggtgca actgcacgtt ttgttacaac gtcacgaatt ttccaccctg
     6001 acgttgcttg acctcttcac ccacccttct cctgctgccc tggccgatta tctggccggc
     6061 gtcgccacgg tggagaaaac aaaacgacct cgccctgttc gccgtcgtca gcggcggata
     6121 tagcgcgaag caaactgatt ttccccggaa cgccatcgcg aacgcatggc gttccattga
     6181 ctttgtgaat cttaggaaac gggaccgatt atggataact tgcgcttctc ttctgcgccg
     6241 acagcagatt ccattgatgc atcgatcgct caacactacc cggactgcga acctgtcgcg
     6301 gttatcggct acgcctgcca ttttcctgaa tcgccggatg gcgaaacgtt ctggcaaaat
     6361 ctgctggaag gtcgtgaatg cagccgacgc tttacgcgcg aagagcttct ggccgtcggt
     6421 ctggatgccg ccatcattga cgatcctcat tatgtcaata tcggtacggt gttagacaac
     6481 gccgactgct tcgacgccac cctgtttggc tattcgcgac aggaagcgga gtcgatggac
     6541 ccgcagcagc gcctgttttt gcaggcggtc tggcatgcgc tggaacatgc cggttatgcc
     6601 cccggcgccg tcccccataa gaccggcgtt ttcgcctctt cccggatgag tacctacccc
     6661 ggtcgcgaag cattgaacgt gacagaagtc gcgcaggtaa aaggtctgca atctctgatg
     6721 ggcaatgata aagactatat tgccacccgc gccgcgtaca aactcaacct gcacggcccg
     6781 gcgttatcgg tacagaccgc ctgctccagc tcgctggttg ccgtgcatct ggcctgtgaa
     6841 agcctgcgcg caggcgaatc cgatatggcg gttgccggcg gcgtggcgct ctctttcccc
     6901 cagcaggcag gctaccgcta ccagcccgga atgattttct ctcctgatgg tcactgtcgt
     6961 ccctttgacg cctcggctga gggcacctgg gccggtaacg gtctcggctg cgtggtgctg
     7021 cgtcgcctga gagacgcgct gctgtcaggc gatccgatta tctcggtgat cctctccagc
     7081 gcggtcaaca acgacggcaa cagaaaggtc ggttataccg ccccttccgt cgcagggcaa
     7141 caagcagtca tcgaagaggc gttaatgctg gcggccatcg acgacaggca ggtaggttac
     7201 attgaaaccc acggcaccgg cacaccgctg ggcgacgcga ttgaaattga agcgttacgc
     7261 aacgtctatg cgcctcgccc gcaggatcag cgctgtgcgc tcggttccgt gaaaagtaac
     7321 atgggccatc tggataccgc ggcgggcatt gccggactgc tgaaaaccgt tctggcagtc
     7381 agtcgcgggc aaattcctcc cttactgaat tttcacaccc ccaacccggc gctgaaactt
     7441 gaagagagcc cctttaccat accggtgtcg gcacaggcat ggcaggacga aatgcgctat
     7501 gcgggcgtct cctcctttgg tattggcggc accaactgcc atatgatcgt cgcctcgctg
     7561 cccgacgcgc tcaacgcgcg cctccccaat acggatagcg gcagaaaaag taccgcgctg
     7621 ctgctcagcg ccgccagcga cagcgcgttg cggcggctgg cgacggatta tgccggggcg
     7681 ctgagagaga atgcggatgc cagctctctg gccttcacag ccctgcacgc gcgccgtctc
     7741 gatctcccct tccgcctggc ggcgccatta aaccgtgaaa ccgccgaggc gctcagcgcc
     7801 tgggccggtg agaaatcggg ggcgctggtt tacagcggcc acggcgccag cggcaagcag
     7861 gtgtggctgt ttaccggcca gggctcgcac tggcgcacta tgggtcaaac gatgtaccag
     7921 cactcaacgg cgtttgccga cacgctggat cgctgttttt ccgcctgtag cgaaatgctc
     7981 acgccgtcac tgcgcgaagc gatgtttaac cccgattcgg cgcagctgga caatatggcc
     8041 tgggcgcagc cggcgattgt cgcgtttgaa atcgcgatgg cggcgcactg gcgtgctgaa
     8101 ggactgaagc cagacttcgc cattgggcat tccgtcggtg aatttgccgc tgccgttgtc
     8161 tgcggacact atacgattga acaggtcatg ccactggttt gtcggcgcgg cgcgctaatg
     8221 cagcagtgcg caagcggcgc gatggtggcg gtatttgcag acgaagacac gctgatgccg
     8281 ctggctcgcc agtttgagct ggatctcgcc gccaacaacg gtacgcaaca tacggtattt
     8341 tccgggccgg aagcccgtct cgcggtattt tgcgccacgc tctcgcagca tgacattaac
     8401 tatcgtcgcc tgagcgtaac cggtgcggcg cactccgctt tactggagcc gatactcgat
     8461 cggttccagg acgcctgcgc gggactgcac gcggagccgg ggcaaatacc gattatttcc
     8521 acgctcaccg ccgacgtcat tgatgagtca acgctcaacc aggcggatta ctggcgccga
     8581 cacatgcgcc agccggtgcg ttttatccag agtattcagg tggcgcatca gctcggcgcc
     8641 cgcgtttttc tggagatggg gcccgatgcc cagttggttg cttgcgggca gcgcgaatac
     8701 cgcgataacg catactggat agccagcgcc cggcgtaaca aagaggcgag cgatgtcctc
     8761 aatcaggccc tgctccagct ttacgctgcc ggcgtcgccc taccgtgggc cgacctgctg
     8821 gcgggcgatg gacaacgtat cgctgcgcca tgttatccgt ttgatactga acgttactgg
     8881 aaagagcgcg tctccccggc ctgcgagcct gccgacgcag cgctgtctgc cgggctggag
     8941 gtggcgagtc gcgccgcgac agcgctcgat ctccctcgcc tggatgcgct taaacagtgc
     9001 gccacgcgac tgcacgccat ctacgtcgat caactggtac aacgctgtac cggcgatgcc
     9061 attgagaacg gcgtggacgc catgaccatc atgcgccgtg gacgtctgct gccccgctac
     9121 cagcagctac tccagcgcct gctgaataac tgcgtggtcg acggcgatta ccgctgcacc
     9181 gacgggcgat acgtccgcgc ccgccccatt gaacatcaac agcgggaatc actgctgacg
     9241 gaacttgccg gttattgtga aggttttcag gctattcccg acaccatcgc ccgtgccggc
     9301 gatcggttat atgaaatgat gagcggcgcg gaagaaccgg tggcgattat cttcccgcaa
     9361 agcgcctccg acggcgtgga agtgctgtat caggaattca gctttggccg ctatttcaac
     9421 caaatcgccg ccggggtatt acgcggcatt gtccagacgc gtcagccccg ccagccgttg
     9481 cgtattcttg aagttggcgg cggaaccggc ggcaccaccg cgtggctgct gccggaactc
     9541 aacggcgttc cggcactgga gtaccatttc accgatatct cggcgctgtt cacccgtcgc
     9601 gcccagcaga aattcgccga ctatgatttt gtgaagtata gcgagctgga tctcgaaaaa
     9661 gaggcgcagt ctcagggttt ccaggcacag tcttacgatc ttatcgtggc agcgaacgtg
     9721 attcacgcca cccgccatat tggccgcacg ctcgataatc tgcgccccct gctcaagccg
     9781 ggcgggcgcc tgctgatgcg cgaaatcacc cagccaatgc gtctgtttga cttcgttttc
     9841 ggcccgctgg ttcttccgct acaggatctc gacgcccgcg aaggtgagtt attcctcacc
     9901 accgctcagt ggcaacaaca gtgccgccac gccggattca gcaaagtggc gtggctaccg
     9961 caggatggca gcccgaccgc cgggatgagc gaacatatca ttctcgccac gctgcccggt
    10021 caggcggtta gcgccgtaac attcaccgcg ccatcagaac ccgtgttggg gcaggcgctg
    10081 acggataacg gtgattatct cgccgactgg tctgattgcg caggtcagcc cgaacggttt
    10141 aacgcccgct ggcaggaggc atggcgtctg ctttcacagc gtcatggcga cgctctgcct
    10201 gtggaaccgc cccccgtcgc cgccccggag tggctgggga aggttcgctt aagctggcaa
    10261 aacgaagcct tttcccgcgg tcagatgcgc gttgaagccc gtcatcctgc tggcgagtgg
    10321 ctgccgctat cgcccgccgc gcctcttcct gcgccgcaaa cgcattatca atggcgctgg
    10381 acgcccctca acgtcgccag cattgaccat ccgcttacct ttagcttcag cgccggtacg
    10441 cttgcgcgca gcgacgagct ggcgcaatac ggcatcattc acgatccgca cgcctcttca
    10501 cgactgatga ttgttgagga gagcgaggat acgctggcct tagcggagaa agtgatagca
    10561 gcgctcaccg ccagcgcagc cggattgatt gtggttactc gccgcgcgtg gcgagtcgag
    10621 gaaaatgaag cactctctgc atcccatcac gcgctatggg ccttgcttcg cgtcgcggcc
    10681 aacgaacagc cggaacggtt gcttgccgcc atcgatctcg ccgaaaacac cccgtgggaa
    10741 acgctgcatc aagggttgag cgcagtctca ctatcacagc gctggctcgc cgcacggggt
    10801 gacacccttt ggctcccttc actgacgccc aatacgggat gcgccgctga attaccggca
    10861 aacgtgttta ccggcgatag ccgctggcat ctggtgaccg gagcgtttgg cggattaggc
    10921 cgccttgccg tgaactggct cagagaaaaa ggggcgcgac gcatcgccct gctggcaccg
    10981 cgcgtggatg agtcatggct acgcgacgtg gagggcgggc agacgcgcgt ctgccgttgt
    11041 gatgtgggcg atgccgggca actggccacg gttcttgacg atctggcggc caacggcggc
    11101 attgccggag cgattcatgc cgctggcgta ttggctgacg cgcccttgca ggagcttgat
    11161 gaccaccagc tggctgccgt tttcgcggta aaagcgcagg cggcaagcca actgttgcaa
    11221 accctgcgca accacgacgg acgctatctt attctctact cttccgctgc cgccaccctc
    11281 ggcgcgccgg gtcagagcgc ccatgcgctg gcctgcggct acctggacgg gctggcccag
    11341 cagttttcca cccttgatgc gccgaaaacg ctctctgtcg cctggggcgc atggggagaa
    11401 agcggtcggg cggccacgcc ggaaatgctg gcgacgctcg ccagccgagg tatgggcgcg
    11461 ttaagcgatg ccgaaggctg ctggcacctg gaacaggcgg tgatgcgcgg cgccccgtgg
    11521 cgactggcga tgcgcgtttt taccgacaaa atgcccccgt tacaacaggc tctgtttaac
    11581 atcagcgcca cagaaaaagc cgcaacgccg gtcattcctc ctgctgatga caacgccttt
    11641 aacggcagcc tgagcgatga aacagcggtg atggcatggc tgaaaaagcg gattgcggtt
    11701 cagctaaggc tgagcgatcc ggcgtcactg catccaaacc aggatctgtt gcaactcggc
    11761 atggactcgc tgctcttcct tgaactcagt agcgatattc agcactacct gggtgtacgc
    11821 atcaatgcgg aacgggcgtg gcaggatctg tctcctcatg gactcacgca gcttatctgt
    11881 tctaagccag aggcgacgcc tgccgcttcg cagccggaag tattgcggca cgacgccgac
    11941 gagcgttatg cgcccttccc tttgacgccc attcagcacg cctactggct ggggcgaacc
    12001 cacctcattg gctatggcgg cgtcgcctgt cacgtcctgt ttgagtggga taaacgccac
    12061 gatgagttcg atctcgccat actggagaaa gcatggaacc agctcatcgc acgccacgat
    12121 atgttgcgta tggtggttga tgccgacggg cagcagcgaa tcctggcgac aacgccggag
    12181 tatcacatcc cgcgtgacga tctgcgcgcg ctttccccgg aagaacagcg catcgcactg
    12241 gaaaaacggc ggcatgaact gagctatcgc gttttgcctg ccgaccagtg gcctcttttt
    12301 gagctggtgg tcagcgaaat cgacgattgc cattaccgtc tgcatatgaa cctcgacctt
    12361 ttgcagtttg atgtgcagag ttttaaagtc atgatggacg acctggcgca ggtctggcgc
    12421 ggtgaaacgc tggcgccgct cgctattacc ttccgtgatt atgtgatggc tgaacaggcg
    12481 cgccgacaga catcggcatg gcacgatgcc tgggattact ggcaggaaaa actgccgcaa
    12541 ctgcccttag cgccagagct gccggtggtt gagacgcccc cggaaacgcc acacttcacc
    12601 accttcaaat cgacgatcgg caagacagaa tggcaggccg tgaaacagcg ctggcagcag
    12661 caaggcgtca caccgtctgc cgcgctgctc acgctgtttg ccgccaccct tgagcgctgg
    12721 agccgtacca caacatttac gctgaacctg acgttcttca atcgccagcc gatccatccg
    12781 caaatcaacc agttgattgg tgattttacc tccgtcacgc tggttgattt taacttctca
    12841 gcgccggtga cgttgcaaga gcagatgcaa cagacccaac agcgcctctg gcaaaacatg
    12901 gcgcacagtg aaatgaacgg tgttgaggtg atccgtgagc tgggccgcct gcgcggatca
    12961 caacgtcaac cgctgatgcc ggtagtgttt accagtatgc tggggatgac gctggaaggc
    13021 atgactatcg atcaggcgat gagccatctg ttcggcgaac cctgctatgt attcacgcaa
    13081 acgccgcagg tctggctgga tcatcaggtc atggagagcg acggcgagtt gatgtttagc
    13141 tggtactgca tggacaacgt gctggaaccc ggcgctgccg aggcgatgtt taatgactat
    13201 tgcgccatcc tgcaagccgt catcgccgcc cctgaaagcc tgaagactct cgccagcggc
    13261 atcgccaggc acattccccg ccgacgctgg ccgctgaacg cgcaggcgga ctacgacctg
    13321 cgggatattg agcaggcgac gctcgaatac cccggcatcc ggcaggccag agcggaaata
    13381 accgaacagg gcgcgttgac gctggatatc gtgatggccg acgatccttc gccatcagcg
    13441 gcgatgcctg atgagcacga acttacccaa ctggcgctgc cgttgcctga gcaggcgcag
    13501 cttgatgagc tggaggcgac ctggcgctgg ctggaggcgc gtgcgctaca ggggatcgcg
    13561 gctacgctaa atcgtcacgg cctgtttacc acgccggaga tcgcccatcg ctttagcgca
    13621 gtagtacagg cgctgtccgc gcaagcgtct caccagcgtc tgctgcgcca gtggctacag
    13681 tgtctgacgg aaagagagtg gttaatccgc gaaggtgaaa gctggcgctg ccgcattccg
    13741 ctcagcgaga ttcctgagcc tcaggaagcg tgcccgcaaa gccaatggag ccaggcgctg
    13801 gcgcagtatc tggaaacctg catcgcccgg cacgacgccc tcttctccgg gcagtgttct
    13861 ccgctggaat tgctgttcaa cgagcagcat cgcgttaccg acgcgctgta tcgcgacaac
    13921 cccgccagcg cctgtctgaa tcgctatacc gcgcagattg ccgccttgtg cagcgcagaa
    13981 cggattctgg aggttggcgc cggaaccgca gccactaccg cgccggtgct gaaggccacg
    14041 cggaacacgc ggcagtcgta ccacttcacg gacgtctccg cgcagttcct caatgacgcc
    14101 agagcccgtt tccatgatga atcgcaggtg tcttatgcct tgttcgacat caaccagccg
    14161 ctggatttca ccgcccaccc ggaggcgggt tacgacctga tcgttgccgt caatgtgctc
    14221 cacgacgcca gccatgtcgt ccagacgttg cgcagattaa aactgttgct gaaagccggc
    14281 ggacgtttgc tgatcgttga agcgacggag cgaaacagcg tattccagct ggcgagcgtg
    14341 ggctttattg agggattaag cggataccgc gatttccgcc gccgggatga gaaaccgatg
    14401 ctcacccgct ccgcatggca ggaggttctc gttcaggccg ggtttgcaaa cgagctggcg
    14461 tggcccgcgc aggaatcgtc gccgctgcgc cagcatctgc tggtagcgcg ttcgcctggc
    14521 gtaaatcgcc cggataaaaa agccgtgagc cgctatttac agcagcgctt tggcaccggt
    14581 ctgcccattt tacagatccg gcaaagagaa gcgttattta cgccgctgca tgccccgtct
    14641 gatgcgccga ctgagccagc caaacccacg ccagttgccg gggggaatcc ggcactggaa
    14701 aaacaggtgg ctgaactctg gcaatcgctg ctgtctcgcc ccgtggcaag gcatcacgac
    14761 tttttcgaac tgggcggcga cagcctgatg gcgacaagga tggtcgcgca gctaaaccgg
    14821 agagggattg ccagggctaa ccttcaggat ctgttcagcc attcgacgct gagcgacttc
    14881 tgcgcccatc tacaggcggc tacgtcagga gaggacaacc cgatacccct ttgccagggc
    14941 gacggtgagg aaaccctgtt tgtcttccac gcttcagacg gcgatatcag cgcctggctg
    15001 ccgctcgcta gcgcgttgaa caggcgcgtt ttcggcctgc aagcaaaatc gccgcagcgc
    15061 tttgccacgc tcgaccagat gatcgatgag tatgtcgggt gcatccgtcg tcagcagcct
    15121 cacggccctt atgtgctggc gggttggtcg tatggcgcgt ttcttgcggc gggcgccgca
    15181 caacgcctgt acgccaaagg cgagcaggtt cggatggtgt taatcgatcc cgtgtgccga
    15241 caggatttct gttgcgaaaa ccgggcggcc ctgctgcgcc tgttagccga aggacaaacg
    15301 cctctggcac tgcccgaaca tttcgaccag cagacgcccg acagccagct tgccgacttt
    15361 atcagcctcg ctaaaacggc cggtatggtg tcgcaaaacc tgacgctgca agcggcagaa
    15421 acgtggctcg acaacatcgc gcatctgctg cgtttactga ctgagcatac gccgggcgaa
    15481 agcgttccgg tcccctgtct catggtgtat gccgccggga gacccgcgca ctggacgcca
    15541 gcagaaaccg agtggcaggg ctggataaac aacgccgacg acgctgtgat tgaagccagc
    15601 cactggcaaa tcatgatgga agccccccac gttcaggctt gtgcgcaaca cattacgcgc
    15661 tggctttgcg caacctcaac gcaaccggag aacacgttat gatgccgtcc gcctccccaa
    15721 aacaacgcgt actgattgtg ggcgccaaat ttggcgaaat gtacctgaat gcctttatgc
    15781 agcccccgga ggggctggaa ctggtcggcc tgctggcgca gggaagcgcc cgttcaagag
    15841 agctggctca tgcgtttggc attccgctgt atacctcgcc ggaacagata accaggatgc
    15901 cggatatcgc ctgtattgtc gtgcgttcga ccgtcgccgg aggaaccggt acgcagcttg
    15961 ccagacactt tctgacgcgc ggcgtacacg tcattcagga gcatcccctc cacccggatg
    16021 acatcagttc cttacaaacg ctggcgcagg aacagggctg ctgctattgg gtaaacacgt
    16081 tttatccgca tacgcgcgcg ggacgcacat ggcttcgcga tgcgcagcag ctgcgtcgct
    16141 gtctggctaa gacgccgcag gtggtccacg ccaccaccag ccgacagttg ctctactcca
    16201 ctctggattt gttgttgctg gctctgggcg ttgatgccgc cgccgtggag tgcgacgtgg
    16261 ttggcagctt tagcgatttt cactgtctga gactcttctg gccggaggga gaagcctgcc
    16321 tgctgcttca gcgctatctc gatcctgacg atccagatat gcatagcctt atcatgcacc
    16381 gcctgctgct gggctggccg gaaggtcatc tttcgctgga ggcgagctac ggccccgtca
    16441 tctggtcatc cagcctgttt gtcgcagacc atcaggagaa cgcccacagc ctctaccgca
    16501 gaccggagat cctgcgcgat ctgccgggtc tgacgcgcag cgcggcgccc ctgagctggc
    16561 gcgactgctg cgaaaccgtc gggccggagg gcgtcagctg gttgctacac cagttgcgca
    16621 gccatctggc gggcgaacat ccaccagcgg cctgccaaag cgtacatcag atcgcgttat
    16681 cacgtttatg gcagcagatt ttacgtaaga ccggcaatgc ggagatcaga cgcctgacgc
    16741 cgccgcacca cgaccggtta gccggtttct acaacgatga tgataaggag gcgctgtgac
    16801 gcaatctgca atgtgcatcc cgctgtggcc cgcccggaac ggcaatactg cgcatctggt
    16861 catgtgccct ttcgctggcg gcagcagtag cgcgtttcgc cactggcaag ctgagcaact
    16921 ggctgattgc gcgctttctc tggtgacctg gccggggcgc gatcgccttc gccatctgga
    16981 accgctcaga aacattacac aactggcggc actgctggcg aacgagctgg aagcatccgt
    17041 atcgcctgac acgccgcttt tactcgccgg gcacagcatg ggggcgcagg tggcgtttga
    17101 aacctgccga cttctggagc aacgggggct tgcgccacaa ggactgatta tttccgggtg
    17161 ccatgccccg catctgcatt ctgaacgcca gctcagccat cgcgatgatg ccgactttat
    17221 cgctgagctg atagacattg gcggatgttc tcctgaactg cgggaaaacc aggaattaat
    17281 gtcgctgttt cttcctcttc tgcgcgctga tttttacgcc accgagagct atcactacga
    17341 ctcgcccgac gtctgtccgc cgctgcgcac gcctgcgctg ttattgtgcg gcagccacga
    17401 tcgcgaagcc tcctggcagc aggtcgatgc ctggcgtcag tggctgagcc acgttacagg
    17461 cccggtggtg attgacggcg atcatttcta tcccattcaa caagcccggt ccttttttac
    17521 gcagattgtc cgccattttc cccacgcatt ttctgcaatg accgcgtggc aaaaacagcc
    17581 cagtacttca gaaaggtgac gcatgaattc ttcctttgaa tctctgattg aacagtatcc
    17641 cttacccatt gccgaacagt tgcgccactg ggcggcccgt tatgcctcgc gaattgccgt
    17701 cgttgatgca aaggggtcgt taacctacag cgcgcttgat gcacaagttg acgaacttgc
    17761 cgcaggtctg tcatcactgg gtttgcgttc gggggagcat gtaattgtgc agcttcccaa
    17821 cgacaacgcg tttgttaccc tgctgttcgc cttgttaaga ctgggcgtta tccccgtgct
    17881 ggcgatgccc tcgcaacggg ctctggatat cgacgcgctg attgagctgg cgcaacccgt
    17941 cgcttacgtt attcacgggg aaaacctcgc agagctggcc cgacagatgg cgcacaaaca
    18001 cgcctgcttg cgtcatgttc tggtcgctgg agagaccgtg agcgacgatt ttacgccgct
    18061 cttctccctt cacggtgagc gacaggcatg gccgcagcct gatgtttccg ccaccgcgtt
    18121 gttgttgctc tcaggcggca caaccggcac gcccaaactc atcccgcgcc gacatgccga
    18181 ctatagctat aacttcagcg cttctgctga actgtgcggc atcagccaac agagcgtgta
    18241 tctcgccgtc ctcccggtgg cgcataactt tccgctggcc tgccccggta ttctgggaac
    18301 gcttgcctgc ggcggaaaag tggtgctgac cgacagcgcc agctgtgatg aggtgatgcc
    18361 tttaatcgcg caggaaagag tgactcacgt cgccctggtt ccggcgctgg cgcaattatg
    18421 ggtgcaggcc agggagtggg aagacagcga cctttcgtcg ctgcgcgtca ttcaggcagg
    18481 cggcgcccgg ctcgacccga cgcttgctga gcaggttatc gccacctttg actgtaccct
    18541 gcaacaggtt ttcggtatgg cggaaggcct gctctgtttt acccggctgg acgatccgca
    18601 tgccaccatt ctccacagcc aggggcgccc gttgtcccct ctggatgaaa tccgcatcgt
    18661 tgatcaagac gagaacgacg tcgcgccggg cgaaaccggg caattgttaa cgcgcggccc
    18721 ttataccatt tcgggctatt accgcgcccc tgcccacaac gcgcaggcct ttaccgcgca
    18781 agggttttac cgcacaggcg acaatgtcag gctggatgag gtggggaacc tgcacgttga
    18841 gggacgcata aaagagcaga tcaaccgcgc cggagaaaaa atagccgcgg ctgaagtgga
    18901 atcggcactg ctgcgtttag cggaagtgca agattgcgcg gtggtcgccg cgccggacac
    18961 gctgcttggc gagcggattt gcgcgtttat catcgcgcag caggtgccaa ctgactatca
    19021 gcagttgcgt caacaactga cccgtatggg gctcagcgcg tggaaaattc ctgaccaaat
    19081 cgagtttctg gaccactggc cgctcaccgc cgtcggcaag atagacaaaa aacgcctgac
    19141 ggctctcgcc gtcgaccgtt atcgccattc tgcccaataa gcgcaaaccg acccgaaaca
    19201 ggttgaaata aacccgtttc gggtagcacc actattagaa atagttatca ttttcaattc
    19261 accattgtcg gtatttttgg cgtttcgccg tcttacaggg actcacaaca atgaaaatga
    19321 cacggcttta tcctctggcc ttggggggat tattgctccc cgccattgct aatgcccaga
    19381 cttcacagca agacgaaagc acgctggtgg ttaccgccag taaacaatct tcccgctcgg
    19441 catcagccaa caacgtctca tctactgttg tcagcgcgcc ggaattaagc gacgccggcg
    19501 tcaccgccag cgacaaactc cccagagtct tgcccgggct caatattgaa aatagcggca
    19561 acatgctttt ttcgacgatc tcgctacgcg gcgtctcttc agcgcaggac ttctataacc
    19621 ccgccgtcac cctgtatgtc gatggcgtcc ctcagctttc caccaacacc atccaggcgc
    19681 ttaccgatgt gcaaagcgtg gagttgctgc gaggcccaca gggaacgtta tatggcaaaa
    19741 gcgctcaggg cgggatcatc aacatcgtca cccagcagcc ggacagcacg ccgcgcggct
    19801 atattgaagg cggcgtcagt agccgcgaca gttatcgaag taagttcaac ctgagcggcc
    19861 ccattcagga tggcctgctg tacggcagcg tcaccctgtt acgccaggtt gatgacggcg
    19921 acatgattaa ccccgcgacg ggaagcgatg acttaggcgg cacccgcgcc agcataggga
    19981 atgtgaaact gcgtctggcg ccggacgatc agccctggga aatgggcttt gccgcctcac
    20041 gcgaatgtac ccgcgccacc caggacgcct atgtgggatg gaatgatatt aagggccgta
    20101 agctgtcgat cagcgatggt tcaccagacc cgtacatgcg gcgctgcact gacagccaga
    20161 ccctgagtgg gaaatacacc accgatgact gggttttcaa cctgatcagc gcctggcagc
    20221 agcagcatta ttcgcgcacc ttcccttccg gttcgttaat cgtcaatata cctcagcgct
    20281 ggaatcagga tgtgcaggag ctgcgcgctg caaccctggg cgatgcgcgt accgttgata
    20341 tggtgtttgg gctgtaccgg cagaacaccc gcgagaagtt aaattcagcc tacgacatgc
    20401 cgacaatgcc ttatttaagc agtaccggct ataccaccgc tgaaacgctg gccgcataca
    20461 gtgacctgac ctggcattta accgatcgtt ttgatatcgg cggcggcgtg cgcttctcgc
    20521 atgataaatc cagtacacaa tatcacggca gcatgctcgg caacccgttt ggcgaccagg
    20581 gtaagagcaa tgacgatcag gtgctcgggc agctatccgc aggctatatg ctgaccgatg
    20641 actggagagt gtatacccgt gtagcccagg gatataaacc ttccgggtac aacatcgtgc
    20701 ctactgcggg tcttgatgcc aaaccgttcg tcgccgagaa atccatcaac tatgaacttg
    20761 gcacccgcta cgaaaccgct gacgtcacgc tgcaagccgc gacgttttat acccacacca
    20821 aagacatgca gctttactct ggcccggtcg ggatgcagac attaagcaat gcgggtaaag
    20881 ccgacgccac cggcgttgag cttgaagcga agtggcggtt tgcgccaggc tggtcatggg
    20941 atatcaatgg caacgtgatc cgttccgaat tcaccaatga cagtgagttg tatcacggta
    21001 accgggtgcc gttcgtacca cgttatggcg cgggaagcag cgtgaacggc gtgattgata
    21061 cgcgctatgg cgcactgatg ccccgactgg cggttaatct ggtcgggccg cattatttcg
    21121 atggcgacaa ccagttgcgg caaggcacct atgccaccct ggacagcagc ctgggctggc
    21181 aggcgactga acggatgaac atttccgtct atgtcgataa cctgttcgac cgtcgttacc
    21241 gtacctatgg ctacatgaac ggcagcagcg ccgtcgcgca ggtcaatatg ggtcgcaccg
    21301 tcggtatcaa tacgcgaatt gatttcttct gattattgta aaagggatac cgaaaaggta
    21361 tcccttttac accactagtt aaaaccagta actcagcaga gtcgcaaaaa ta
//
LOCUS       DADSZD010000077        21129 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.077, whole genome shotgun sequence.
ACCESSION   DADSZD010000077 DADSZD010000000
VERSION     DADSZD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.077"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            81..524
                     /gene="yjdN"
                     /locus_tag="J1Z28_002512"
     CDS             81..524
                     /gene="yjdN"
                     /locus_tag="J1Z28_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBA7717339.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVIKQQPTQ"
     gene            657..1445
                     /gene="phnC"
                     /locus_tag="J1Z28_002513"
     CDS             657..1445
                     /gene="phnC"
                     /locus_tag="J1Z28_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBA7717340.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            1470..2486
                     /gene="phnD"
                     /locus_tag="J1Z28_002514"
     CDS             1470..2486
                     /gene="phnD"
                     /locus_tag="J1Z28_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7717341.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            2592..3371
                     /gene="phnE"
                     /locus_tag="J1Z28_002515"
     CDS             2592..3371
                     /gene="phnE"
                     /locus_tag="J1Z28_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBA7717342.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLIVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3392..4117
                     /gene="phnF"
                     /locus_tag="J1Z28_002516"
     CDS             3392..4117
                     /gene="phnF"
                     /locus_tag="J1Z28_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBA7717343.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4118..4570
                     /gene="phnG"
                     /locus_tag="J1Z28_002517"
     CDS             4118..4570
                     /gene="phnG"
                     /locus_tag="J1Z28_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBA7717344.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            4567..5151
                     /gene="phnH"
                     /locus_tag="J1Z28_002518"
     CDS             4567..5151
                     /gene="phnH"
                     /locus_tag="J1Z28_002518"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBA7717345.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVNQPKQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5151..6215
                     /gene="phnI"
                     /locus_tag="J1Z28_002519"
     CDS             5151..6215
                     /gene="phnI"
                     /locus_tag="J1Z28_002519"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBA7717346.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEYSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6208..7053
                     /gene="phnJ"
                     /locus_tag="J1Z28_002520"
     CDS             6208..7053
                     /gene="phnJ"
                     /locus_tag="J1Z28_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBA7717347.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            7050..7808
                     /gene="phnK"
                     /locus_tag="J1Z28_002521"
     CDS             7050..7808
                     /gene="phnK"
                     /locus_tag="J1Z28_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBA7717348.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            7919..8599
                     /gene="phnL"
                     /locus_tag="J1Z28_002522"
     CDS             7919..8599
                     /gene="phnL"
                     /locus_tag="J1Z28_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBA7717349.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLNTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            8596..9732
                     /gene="phnM"
                     /locus_tag="J1Z28_002523"
     CDS             8596..9732
                     /gene="phnM"
                     /locus_tag="J1Z28_002523"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBA7717350.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRRVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAM
                     CRARHIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            9732..10289
                     /gene="phnN"
                     /locus_tag="J1Z28_002524"
     CDS             9732..10289
                     /gene="phnN"
                     /locus_tag="J1Z28_002524"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBA7717351.1"
                     /translation="MTGKLIWLMGASGSGKDSLLTELRQREQTQLLVAHRYITRAASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLSLIHQKEKHHACL"
     gene            10276..10710
                     /gene="phnO"
                     /locus_tag="J1Z28_002525"
     CDS             10276..10710
                     /gene="phnO"
                     /locus_tag="J1Z28_002525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBA7717352.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            10712..11470
                     /gene="phnP"
                     /locus_tag="J1Z28_002526"
     CDS             10712..11470
                     /gene="phnP"
                     /locus_tag="J1Z28_002526"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBA7717353.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCDCVACARARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDTIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVA"
     gene            complement(11498..11827)
                     /gene="yjdP"
                     /locus_tag="J1Z28_002527"
     CDS             complement(11498..11827)
                     /gene="yjdP"
                     /locus_tag="J1Z28_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBA7717354.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQRQVSDDRRKQYNDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(11896..12345)
                     /gene="rpiB"
                     /locus_tag="J1Z28_002528"
     CDS             complement(11896..12345)
                     /gene="rpiB"
                     /locus_tag="J1Z28_002528"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HBA7717355.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERDVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            12704..13594
                     /gene="alsR"
                     /locus_tag="J1Z28_002529"
                     /gene_synonym="rpiR"
     CDS             12704..13594
                     /gene="alsR"
                     /locus_tag="J1Z28_002529"
                     /gene_synonym="rpiR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="als operon DNA-binding transcriptional repressor
                     AlsR"
                     /protein_id="HBA7717356.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            13653..14588
                     /gene="alsB"
                     /locus_tag="J1Z28_002530"
     CDS             13653..14588
                     /gene="alsB"
                     /locus_tag="J1Z28_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HBA7717357.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANARKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            14716..16248
                     /gene="alsA"
                     /locus_tag="J1Z28_002531"
     CDS             14716..16248
                     /gene="alsA"
                     /locus_tag="J1Z28_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HBA7717358.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTIKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAEAQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCHPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     HE"
     gene            16227..17207
                     /gene="alsC"
                     /locus_tag="J1Z28_002532"
     CDS             16227..17207
                     /gene="alsC"
                     /locus_tag="J1Z28_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HBA7717359.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17218..17913
                     /gene="rpe"
                     /locus_tag="J1Z28_002533"
     CDS             17218..17913
                     /gene="rpe"
                     /locus_tag="J1Z28_002533"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBA7717360.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            17897..18826
                     /gene="alsK"
                     /locus_tag="J1Z28_002534"
     CDS             17897..18826
                     /gene="alsK"
                     /locus_tag="J1Z28_002534"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HBA7717361.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQSRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19099..21084
                     /gene="yjcS"
                     /locus_tag="J1Z28_002535"
     CDS             19099..21084
                     /gene="yjcS"
                     /locus_tag="J1Z28_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBA7717362.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATNEAKAATQYTQQVN
                     QNYAKSLPFSNRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIPPTKTIVRTGDKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYMQALGGSARVINLAKEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSEKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5043 a         5839 c         5626 g         4621 t
ORIGIN      
        1 cgcgtcttat caggcttccc tctcatctgt ataaatttcg aactacactt aacgggcttc
       61 tcttaactga ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtg
      121 ccgacgcaat tgcttattat caacgtacgt tgggcgctga actgctctat aaaatcagct
      181 tcggcgaaat gccgaaatca gcgcaggaca gcgccgaaaa ctgcccttcc ggaatgcaat
      241 ttcccgatac cgccatcgcg catgccaacg tgcgcattgc cggaagcgac atcatgatga
      301 gcgatgccat gccgtcagga aaagccagct actccggctt tacgctggtg ctcgattcgc
      361 aacaggtgga agaaggaaaa cgctggtttg ataaccttgc cgccaacgga aaaatcgaaa
      421 tggcctggca ggaaacgttc tgggcgcatg gctttggcaa agtcaccgat aaatttggcg
      481 taccgtggat gattaatgtc atcaaacaac agccaacgca ataacccgcc gggaggcccg
      541 ccctcccgca ctgtcatcga attcccgtta actcttcatc tgttagtcac ttttaattaa
      601 ccaaatcgtc acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc
      661 aaacgattat ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcag gcgctgcacg
      721 cggttgatct gaacattcat catggtgaaa tggtggctct gcttgggccg tcgggttccg
      781 gaaaatccac ccttttacgt cacttaagcg gtttgattac cggcgataaa tctgtcggta
      841 gccatatcga gctgctgggc cgcactgtcc agcgcgaagg ccgtctggcc cgcgatatcc
      901 gcaaaagccg cgcccatacc ggctacatct ttcaacagtt caacctggtg aatcgcctga
      961 gcgtactgga gaacgtgctg attggcgcgc tcggcagcac gccattctgg cgaacctgtt
     1021 ttagctactt cacccgcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca
     1081 tggtgcactt tgcccatcaa cgcgtttcca cactctccgg cggccagcaa cagcgtgtgg
     1141 cgattgcccg cgcgctgatg cagcaggcga aggtcattct cgctgacgaa cccatcgcct
     1201 cgctggaccc ggaatcggca cgcatcgtga tggacaccct gcgcgacatc aaccagaacg
     1261 acggcattac cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac
     1321 gcatcgtcgc cctgcgccag gggcacgtct tctacgacgg ctgcagccaa cagtttgata
     1381 acgaacgttt tgaccatctc taccgcagca ttaaccgcgt cgaagagaac gcgaaagctg
     1441 cctgacatcc ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct
     1501 tcaccagcat gttcagcctc agcaccctgt taaatccggc atacgccgaa gagcaggaaa
     1561 aggcgctgaa tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga
     1621 cgccgttctt acaggatatg gagaagaagc tgggcgtaaa ggtcaacgcc ttctttgccc
     1681 cggactacgc gggcattatc caggggatgc gcttcaataa agtggatatc gcctggtacg
     1741 gcaacctgtc ggcgatggaa gcggtggatc gcgccaacgg tcaggtcttc gcccagacgg
     1801 tcgcggcgga tggatcgccg ggttactgga gcgtgttgat cgtcaacaaa gatagcccga
     1861 tcaacaacct gaacgatctg ctggcgaagc ggaaagatct caccttcggc aatggcgatc
     1921 ctaactctac ctccggcttc ctcgtccccg gttactacgt cttcgctaaa aacaatatct
     1981 ccgccagcga ctttaagcgc accgtcaacg ccgggcatga aaccaacgcc ctcgccgtcg
     2041 ctaacaagca agtggatgtc gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct
     2101 ccgcgccgga gaagctgaaa gaactgaagg tgatctggaa atcaccgctg atccctggcg
     2161 atccgatcgt ctggcgcaaa aatctttccg aaaccactaa agacaagatc tacgacttct
     2221 ttatgaatta cggcaaaacg ccggaagaga aagcggtgct ggagcgcctg ggctgggcgc
     2281 cgttccgcgc ctccagcgac ttgcaactgg tgccgattcg ccagctcgca ctgtttaaag
     2341 agatgcaaag cgtgaaggac aacaaagggc tgaacgaaca ggacaagctg gcaaaaacca
     2401 ccgccattca ggcgcaactg gatgatttgg accgcctgaa caacgcgtta agcgcaatga
     2461 gttcggtgag taaagcggtg cagtaaatcg taggtcggat aagacgcccc ggcgtcgcat
     2521 ccgacaatgt gcagacgttg atgccggatg cggtgcaagc accttatccg gcctacagac
     2581 cggagccaaa catgcaaacc atcaccatcg ccccacccaa gcgcagctgg ttctcgcttc
     2641 tgagctgggc cgttgttatc gccgtgctga tcgtctcgtg gcagggcgcg gaaatggccc
     2701 cgcttacgct gattaaagat ggcggcaaca tggcgacgtt cgccgccgac ttcttcccgc
     2761 ccgatttcag ccagtggcag gattacctca ccgaaatggc cgtcacgctg caaatcgccg
     2821 tctggggcac cgcgctggcg gtggttctct ccatcccctt cggcctgatg agcgctgaaa
     2881 acctggtacc gtggtgggtc taccaacctg tacgccgcct gatggacgcc tgccgcgcca
     2941 ttaacgaaat ggtcttcgcc atgctgttcg tggtcgccgt cggcctcggc ccgttcgctg
     3001 gcgtgctggc gctgtttatc cacaccaccg gcgtactttc caaactgctt tccgaagcgg
     3061 tagaagcgat tgaacccggc ccggtggagg gcattcgcgc taccggtgcc aacaagctgg
     3121 aagagatcct ctacggcgta ctaccgcagg tcatgccgct gctgatttcc tactctctct
     3181 atcgcttcga atccaacgtc cgctcggcga ccgtcgtcgg tatggttggc gcgggcggaa
     3241 ttggcgtcac gctgtgggaa gcgattcgcg gtttccagtt ccagcaaacc tgcgccctga
     3301 tggtgctcat catcgtcacg gtcagcctgc tggatttcct ctctcaacgg ttgcgtaagc
     3361 actttatctg ataagcgagg cattgatatc tatgcacttg tctacacatc cgaccagcta
     3421 cccaacacgc tatcaggaga tagccgcaaa acttgagcag gagcttcgtc aacattaccg
     3481 ctgcggcgac tatcttcccg ccgagcagca actggcggcg cgctttgagg tgaaccgcca
     3541 caccctgcgc cgcgccatcg accaactggt ggaaaaaggc tgggtacagc gccgtcaggg
     3601 cgtcggcgta ctggtgctga tgcgcccgtt cgattacccg ctcaacgccc aggcgcgttt
     3661 tagccagaac ctgctggacc agggcagcca tcccaccagc gaaaaattgc tatcggtgct
     3721 gcgccccgcc tccggtcacg tcgctgacgc gctggggata accgaggggg agaacgtcat
     3781 ccacctgcgc accctgcgtc gggtcaacgg cgtcgcactc tgtttaatcg atcactactt
     3841 cgcagatctc accctctggc caacgcttca atgctttgac agcggttcgc tacacgattt
     3901 tctgcgcgag caaacgggga ttacgctacg acgcagtcag acgcggatca gcgcccgccg
     3961 cgcccaggcc aaagagtgcc agcgtcttga aattccgaat atgtcaccgc tgctgtgcgt
     4021 gcgcaccctt aaccaccgtg acggtgaaag cagcccggcg gaatactccg tcagcctgac
     4081 acgcgccgac atgattgaat tcactatgga gcactgaatg cacgcagata ccgcgacccg
     4141 ccagcactgg atgtccgtgc tggcgcacag ccaaccggct gaactggcag cccgcctgaa
     4201 cgcgctgaac atcaacgccg attatgaggt gatccgcgcc gctgaaactg gcctggtgca
     4261 gattcaggcg cgaatgggcg gcaccggcga acgctttttt gctggcgacg ccacgctcac
     4321 ccgcgccgcc gtgcgtctga ctgacggcac actcggctat agctgggtac tggggcgcga
     4381 caaacagcac gccgaacgtt gtgcgctgat tgatgcgctg atgcagcaaa accgccactt
     4441 tcaaaactta tcagaaaccc ttattacccc gctggacgct gaccgtatgg cacgcattgc
     4501 cgcgcgtcag gccgaagtga acgccagccg ggtcgacttc tttacgatgg ttcgcggaga
     4561 caacgcatga ccctggaaac cgcttttatg cttcccgtgc aggatgccca gcacagtttt
     4621 cgtcgcctgt taaaggccat gagcgagccg ggcgtgattg tcgccctgca tcagctcaag
     4681 cgcggctggc agccgctgaa tatcgccacc accagcgtgc tgctgacgct ggccgataac
     4741 gacacgccgg tgtggcttgc cgcaccattg agcaacgata tcgtcaatca gagcctgcgt
     4801 tttcatacca acgcgccgct ggttaatcag ccgaaacagg cgaccttcgc ggtaacagat
     4861 gaggcgatct ccagcgagca gctcaacgcc ctttccaccg gcactgccgt tgcaccagaa
     4921 gcgggcgcaa cgctgatttt acaggtcacc agcctgagcg gtgggcgtat gttgcgcctc
     4981 accggcgcag gtattgccga agaacggatg atcgccccgc aactgccaaa gtgcattctt
     5041 catgaactta ccgagcgccc gcacccgttc ccgctcggca tcgacctgat cctgacctgc
     5101 ggcgagcgcc tgctggctat tccgcgaacc actcatgtgg aggtgtgctg atgtacgttg
     5161 ccgtgaaagg gggcgaaaag gcgatcgacg ccgcccacgc cctgcaagag agccgacgcc
     5221 ggggcgatac cgatttgcct gaactaagcg tcgcccagat tgaacagcag cttaacctcg
     5281 cggtagatcg cgtgatgacc gaaggcggta ttgccgaccg cgagctggcc gcgctggcgc
     5341 tgaaacaggc cagcggcgat aacgtcgaag cgattttcct gttgcgcgcc taccgtacca
     5401 cgctggcgaa gctggcggtc agtgagccgc tcgacaccac cgggatgcgt ctcgaacggc
     5461 gtatttcagc aatttataaa gacattcccg gcggccaact gcttggcccc acctacgact
     5521 acacccatcg cctgctcgat ttcaccctgc tggcaaacgg cgaaacgcca acgctgacca
     5581 ccgctgacag cgaacagcaa ccgtcgccac atgtcttcag cctgctggcg cgtcaggggc
     5641 tggcgaagtt tgaagaatat agcggcgcac aaccggacga catcacccgc acgccgccgg
     5701 tttatccctg ttcgcgttct tcccgtctac aacaattaat gcgcggtgac gaaggctatt
     5761 tgttggcgct ggcctactcc acccaacgcg gttacgggcg caatcacccg ttcgcaggcg
     5821 aaattcgcag tggctatatc gatatattta ttgtgccgga agagctggga tttgcggtga
     5881 acgtcggcga actgttgatg accgagtgtg aaatggtcaa cggttttatc gacccgccgg
     5941 atgaaccacc gcattttaca cgcggctatg ggctggtgtt cggcatgagc gagcgcaaag
     6001 caatggcgat ggcgctggtt gaccgtgcgt tgcaggcacc ggaatacggc gagcacgcaa
     6061 caggtccggc acaggatgaa gagttcgtgc tggcccatgc cgacaacgtc gaagccgcag
     6121 gctttgtttc gcatctcaaa ctcccgcact acgtcgattt ccaggccgaa ctggagctac
     6181 tcaaacgtct gcaacaggag cagaaccatg gctaatctga gcggctacaa ctttgcttac
     6241 ctcgacgagc agaccaaacg catgatccgc cgcgctatct taaaagcggt ggcaatcccc
     6301 ggttatcagg tgccgtttgg cggacgcgag atgccgatgc cctacggctg gggaaccggc
     6361 ggcattcagc tcaccgccag cgtgattggc gaaagcgacg tgttgaaggt gattgaccag
     6421 ggcgcagacg ataccaccaa cgccgtgtcg attcgtaact tctttaagcg cgtgaccggg
     6481 gtgaacacca ccgagcgcac ggacgatgcg acacttatcc agacgcgtca ccgcataccc
     6541 gaaacgccgc tgaccgaaga tcagatcatt atcttccagg tgccgatccc cgaaccgctg
     6601 cgctttattg agccgcgaga gacggaaacg cgcaccatgc acgcgctgga ggagtacggc
     6661 gtgatgcagg tgaaactgta tgaagatatc gcccgcttcg gtcatatcgc caccacctac
     6721 gcctatccgg tgaaggtgaa cgggcgctac gtaatggacc cgtcgccgat cccgaaattc
     6781 gataacccga aaatggacat gatgcccgcc ctgcaactgt ttggcgcggg gcgcgagaag
     6841 cgcatctatg ccgtgccacc gtttacccgc gtggaaagcc tcgatttcga tgatcatccg
     6901 ttcaccgtcc agcagtggga tgaaccatgc gccatctgcg gatcgaccca cagttacctc
     6961 gacgaagtgg tgctggatga cgccggaaac cgcatgtttg tctgctccga taccgattat
     7021 tgccgccaac agaacgaggc caaaagccaa tgaatcaacc gttactttcg gtcaataacc
     7081 tgacccacct ttatgccccg ggcaaaggct tcagcgatgt ctcttttgat ttatggccgg
     7141 gagaagttct gggcattgtt ggggagtccg gttccgggaa gaccacgcta ctgaaatcaa
     7201 tttccgcccg cctgacacca cagcaagggg aaattcgcta cgagaaccgt tcgctgtacg
     7261 cgatgagcga ggccgaccgc cgtcgcctgc tgcgcaccga atggggtgtg gtgcatcagc
     7321 atccgcttga cggcctgcgc cgccaggtgt cggcaggcgg caatatcggc gaacggttga
     7381 tggcaaccgg agcgcgtcat tacggcgata ttcgcgccac cgcgcagaaa tggctggaag
     7441 aggtggagat tcccgccaac cggatcgacg acctgccgac caccttttcc ggcggcatgc
     7501 agcaacgttt gcagattgcc cgcaacctgg tgacgcatcc gaagctggtg tttatggatg
     7561 aacccactgg cgggctggat gtgtcggtac aggcgcgcct gctggacctg ctccgcggtc
     7621 tggtgatgga gcttaacctg gcggtggtga ttgtcaccca cgatttaggc gtcgcccgtc
     7681 tgctggcgga ccgtttgctg gtaatgaagc aagggaaagt ggtggagagt gggttaaccg
     7741 accgggtgct cgacgacccg caccatccgt atacccagtt gctggtgtca tcggttttgc
     7801 agaattgagc tggtgccgga tgcggcgtaa acgccttatc cggcctacaa atgcgctccc
     7861 cgtaggtcgg ataagacgca tcagcgtcgc atccgacacc cgaaccacga ggcgaaaaat
     7921 gattaacgta caaaacgtca gtaaaacctt catcctgcac cagcaaaacg gcgtgcgcct
     7981 gcctgtatta caaggcgcct cactcaccgt caacgccggc gaatgcgtgg tactccacgg
     8041 ccattccggc agtggaaaat caactctgct acgctcgctg tatgccaact atctacccga
     8101 cgaagggcaa atccagatca aacacggtga tgaatgggtc gacctggtca ccgcgccagc
     8161 gcgcaaagtg gtggagatcc gcaaaaccac tgtcggctgg gtaagccagt ttcttcgcgt
     8221 catccctcgt atttcggcac tggaagtggt gatgcagccg ctgctcaata ccggcgtacc
     8281 gcgtgaagcc tgcgccgcta aagccgcccg attactgacg cgcctgaacg tgcctgaacg
     8341 cctgtggcac cttgcgccat caaccttttc cggcggcgaa cagcagcgtg tcaacatcgc
     8401 ccgtggcttt atcgtcgact accccattct gctgctcgac gaacctaccg cctcgctgga
     8461 cgctaaaaac agcgccgccg tagtggaact gatccgcgaa gccaaagccc gcggcgcggc
     8521 catcgtcggt atcttccatg acgaaaccgt gcgcgagcaa gtcgccgacc gcctgcaccc
     8581 aatgggagtc tcttcatgat tatcaataac gtcaaactgg tgctggaaaa cgaggtggta
     8641 cacggttctc tggagatgca ggacggcgaa atccgcgcct ttgccgaaag ccagagtcac
     8701 ctgtcagaag caatggacgg cgaaggcggc tggctgctac cggggctgat tgagctgcat
     8761 accgataatc tggataaatt cttcaccccg cgcccgaaag ttgactggcc tgcccactcg
     8821 gcgatgagca gccacgacgc gctgatggtg gcgagcggca tcaccaccgt gctggacgcc
     8881 gtggcgattg gtgatgtacg cgacggcggc gatcggctgg agaatctgga gaagatgatc
     8941 aacgccatcg aagagacgca gaaacgccgc gtcaaccgtg ccgagcaccg tctgcaccta
     9001 cgctgcgaac tgccgcatca caccacgctg ccgctgtttg aaaaactggt acagcgcgaa
     9061 ccggtaacgc tggtgtcgct gatggatcac tcaccgggcc agcgccagtt cgccaaccgc
     9121 gaaaaatatc gtgaatatta tcagggcaaa tactcgctca ccgatgcgca gatgcaacag
     9181 tacgaagagg agcaactggc gctcgccgca cgctggtcac agccgaatcg cgaaactatc
     9241 gccgcaatgt gccgcgcgcg acacattgcc ctcgccagcc acgatgacgc cacgcacgcc
     9301 cacgttgccg aatcccacca gcttggcagc gtgatcgccg aatttcccac cacgttcgaa
     9361 gcggcagaag cctcgcgcaa gcatggcatg aacgtgctga tgggcgcgcc gaatatcgtg
     9421 cgcggcggtt cgcactccgg caacgtggcg gccagtgaac tggcacagct tggcctgctg
     9481 gatatcctct cttccgacta ctaccccgcc agcctgctgg atgcggcgtt tcgcgtggcc
     9541 gatgaccaga gcaaccgctt tacgctgccg caggcggtga agctggtgac taaaaatccg
     9601 gcgcaggcgc ttaatcttca ggatcgcggg gtgattggcg agggcaaacg cgccgacctg
     9661 gtgctggcgc atcgcaaggg caaccacatt catatcgacc acgtctggcg tcagggtaaa
     9721 agggtgttct gatgacggga aaactgattt ggttaatggg ggcctctggc tccggaaaag
     9781 acagtctgtt gacggaactc cgccagcggg aacaaactca gctactggta gcgcatcgct
     9841 acatcacgcg cgccgccagc gccggaagtg aaaaccatat cgccctgagc gagcaggagt
     9901 tttttacccg cgcggggcag aaccttctgg ccttaagctg gcacgctaac ggtctgtatt
     9961 atggcgtcgg cgtcgagatt gacctctggc tgcacgctgg atttgacgtg gtggtcaacg
    10021 gctcacgcgc ccatctgccg caggcgcggg cgcgctatca atcggcgctg ctgcccgttt
    10081 gtttacaggt ttcgccagag atcctgcgcc agcgcctgga aaaccgtggt cgtgaaaatg
    10141 ccagtgaaat taacgcccgg ctggcgcgcg ccgcccgcta tactcctcag gattgccata
    10201 cgctcaataa tgacggcagc ctgcgccagt cggtcgacac gctgctgtcg ctgattcatc
    10261 agaaggagaa acaccatgcc tgcttgtgaa cttcgcccgg caacgcagta cgacaccgac
    10321 gcggtttacg cgctgatttg tgagctaaaa caagcggagt ttgaccacca ggcgtttcgc
    10381 gtgggcttta acgccaacct gcgtgacccg aatatgcgct accatctggc gctgttggat
    10441 ggcgaggttg tcggcatgat cggcctgcat ttgcagtttc atctgcatca tgtcaactgg
    10501 atcggcgaaa ttcaggaact ggtggtgatg ccgcaggcgc gcggtctgaa cgtcggcagt
    10561 aaattactgg catgggcaga agaagaagcc cgccaggctg gggccgaaat gaccgaactt
    10621 tccaccaacg tgaagcgcca cgacgcgcac cgtttctatc tgcgcgaagg ctacgaacag
    10681 agccatttcc gcttcacgaa ggcgctgtaa tatgagcctg accctcacgc tcaccggcac
    10741 cggcggcgca cagggcgttc cggcatgggg ctgtgattgc gtggcctgcg ccagagcgcg
    10801 acgctcaccg cagtatcgcc gccagccatg cagcggcgta gtgaagttta acgaggcgat
    10861 caccctgatc gacgccgggc ttcacgatct cgccgatcgc tggtctcccg gatcgttcca
    10921 gcagttttta ctgacgcact accatatgga tcacgtccag gggctgttcc cgctgcgctg
    10981 gggcgtgggc gacacaatcc cggtctacgg tccaccggat gagcagggct gcgacgatct
    11041 gtttaaacat ccgggcctgc ttgatttcag ccacacggtg gagccgtttg tggtgtttga
    11101 tttgcagggg ttgcaggtca cgccgctgcc gcttaaccat tcgaaactca ctttcggcta
    11161 tttactggaa acggcgcaca gccgggtggc gtggctatct gacaccgcag gcctgccgga
    11221 aaaaacgctg aaatttttac gcaataatca gccgcaggta atggtgattg attgcagcca
    11281 cccgccgcgc gaggatgcac cgcgtaatca ctgtgattta aataccgtgc ttgcgctgaa
    11341 tcaggttatc cgctcgccgc gggttattct gacccatatc agccatcagt ttgatgcgtg
    11401 gctgatggaa aacgcgctac catcggggtt tgaggcgggg tttgatggga tggagattga
    11461 ggtggcgtga gggaaaatac ccgcgccggc ccctatatca acgcccatac tcatcttcca
    11521 ttctccgctc ttcatcttcc agttgccgac gctcctgatc aagctggcgc tggcgatcgt
    11581 ccagctgcct gcggcgatct tcaaactggc ggcggcgatc gttatattgc ttgcgacgat
    11641 cgtcgctcac ctggcgttgc caaccgtcgt cgcgcgaatc ttcatagtct cgcccacggt
    11701 cagggttata agcgtcgtta atcgcctgct gaatattgcc aatggtgtcg tcgataatat
    11761 cggcctgggc cggaacggtg gacagcgtga gcagggtgaa taaaagaaat agcggaaagc
    11821 gtttcattag ccaacctcaa aaagaaactc tatccacatt agtcattact catccatgca
    11881 agtagtggat gaatctcaat ttctccgctg ctctattgcc gtaatcgcct ccacgcgttg
    11941 ttgatgacga ccgccttcgt actgtgcgcc cagccacgca tcgacaatca tttttgccag
    12001 ttcgaggcca actactcgtg aaccaaaagc cagcacgttg gtgtcgttat gctgccgcga
    12061 aagttgcgcg gaataaggtt cgctacaaac gaccgcgcga attccggcaa acttgttcgc
    12121 cgctatcgaa ataccgacgc cagtgccaca aatcaaaatc ccgccatcag cctctccgcc
    12181 agcaacggcc agtgcgactt ggctggcgta atgcggataa tcagtacgct ctgacgacca
    12241 ggttccttta tcaatcactt caacgtcacg ctcaactaaa tgtgccacta tttcatgttt
    12301 taaaatgaaa ccgacatgat cacagccaaa tgcaatcttt ttcattcatc ttccccgccc
    12361 gcgcaaaact tcacaatcaa aaaacatttg tgatgtaaat gaattaaaaa cacccaaaat
    12421 cgatgaaatc cgccttcccg atctgtttta cagagttaca tctggcagcg caatagcgtt
    12481 aaagacactc actatccagc aacatctatc atctaataaa ccaggaaaac aaataacatc
    12541 atgtttttaa agtgattaaa tgaaacgaaa ttttaagcca ctctccatta ttcacaacaa
    12601 aacaaacttt ataaatttta tttttttgtg aagttgccag catcttttct gttcttgctg
    12661 tggtgatata gtggcgtctt caattcaagg acaagagaac gtgatgagcc agtcagagtt
    12721 tgattcagcg cttccgaacg gtatcgggtt agcgccttac ctgcgaatga agcaggaagg
    12781 aatgacagaa aacgaaagcc gcatcgtgga gtggttactc aaacccggta acctgagttg
    12841 tgcacccgca attaaagatg tcgcagaagc tctggcggta tctgaagcga tgatcgttaa
    12901 ggtatcaaag ctgctggggt ttagcggctt tcgtaactta cgcagtgcgc tggaagatta
    12961 tttttctcag tcagaacaag tattgccttc cgagttggct tttgatgaag cgccgcagga
    13021 tgtggtgaat aaggtattta acatcacttt acgcaccatt atggaaggtc agtcgatcgt
    13081 caacgttgat gagatccacc gtgccgcccg ctttttctat caggccagac agcgggattt
    13141 gtacggtgcc ggaggatcaa atgctatctg tgctgatgta cagcacaagt ttttgcgcat
    13201 tggcgtacgc tgccaggcct atccggacgc tcacatcatg atgatgtctg cttcgttgtt
    13261 acaggaagga gatgtggtgc tggtggtgac tcattccggg cgaaccagtg atgtaaaagc
    13321 ggctgtagaa ctggcaaaaa agaacggggc aaagattatt tgtataacgc atagctatca
    13381 ttcaccaata gcgaagctgg ccgattatat tatttgctca ccggccccgg aaacaccgtt
    13441 attaggtcgt aatgcctcgg caagaatatt acaactgact ttgctggacg cgttttttgt
    13501 ctctgtcgcc cagctcaata ttgaacaagc taatattaat atgcaaaaaa ccggcgcaat
    13561 tgttgatttc ttctcaccag gcgcgctgaa ataagaaaaa ggtaattccg ccatcgcgga
    13621 gttacctctc cccttaccgt gagaacatga ttatgaataa atatctgaaa tatttcagcg
    13681 gcacactcgt gggcttaatg ttgtcaacca gcgcttttgc tgccgccgaa tatgccgtcg
    13741 tattgaaaac actctccaat ccattttggg tagatatgaa aaaaggcatt gaagatgaag
    13801 caaaaacgct gggcgtcagc gttgatattt ttgcctctcc ttcagaaggc gattttcaat
    13861 ctcaattgca gttatttgaa gatctcagta ataaaaatta caaaggtatc gccttcgcac
    13921 cattatcctc agtgaacctg gtcatgcctg tcgcccgcgc atggaaaaaa ggcatttatc
    13981 tggtcaatct cgatgaaaaa atcgacatgg ataatctgaa aaaagcaggc ggtaatgtgg
    14041 aaggttttgt caccaccgat aatgttgccg tcggggcgaa aggcgcgtca ttcattattg
    14101 acaagctggg tgccgaaggg ggtgaagtcg caatcattga gggtaaagcc ggtaacgcct
    14161 ccggtgaagc gcgtcgtaat ggtgccaccg aagccttcaa aaaagcaagc cagatcaagc
    14221 ttgtcgccag ccagcctgcc gactgggacc gtattaaagc actggatgtc gccactaacg
    14281 tgttgcaacg taatccgaat attaaagcga tctattgcgc gaatgacacg atggcaatgg
    14341 gtgttgctca ggctgtcgca aacgccagaa aaacgggaaa agtgctggtc gtcggtacag
    14401 atggcattcc ggaagcccgc aaaatggtgg aagccggaca aatgaccgcg acggttgccc
    14461 agaacccggc ggatatcggt gcaacgggtc tgaagctgat ggttgacgct gagaaatccg
    14521 gcaaggttat cccgctggat aaagcaccgg aatttaaact ggtcgattca atcctggtca
    14581 ctcaataaaa ctgaatgatg ccggatgatg atgctaataa agagtcttat ccggccaaca
    14641 aaataacctg aacgtagatc tgataagtgt agcccatcag gcaataatac gatatttcgt
    14701 gcagagaggt tatttatggc cacgccatat atatcgatgg cggggatcgg caagtccttt
    14761 ggtccggttc acgcattaaa gtcggttaat ttaacggttt atcctggtga aatacatgca
    14821 ttactaggag aaaatggcgc gggtaaatcc acgctaatga aagttttatc cggaatacat
    14881 gagccgacca aaggcaccat taccattaat aacattaact ataacaagct ggatcataaa
    14941 ttagcggcac aactcggcat cgggattatt tatcaagaac tcagcgttat tgatgaatta
    15001 accgtactgg aaaatttata tattggtcgt catctgacga taaaaatctg tggcgtcaat
    15061 attatcgact ggcgagaaat gcgtgtccgc gccgccatga tgttattacg cgtgggcttg
    15121 aaagttgatt tagatgagaa agtggcgaat ttatctatca gccacaagca gatgctggaa
    15181 attgccaaaa cgctgatgct cgacgccaaa gtcatcatca tggatgaacc cacctcctca
    15241 ctcaccaata aagaggtgga ctatctgttt ctgatcatga atcagttgcg taaggagggt
    15301 acggccatcg tctatatctc gcataaattg gcggaaattc gccgtatttg cgaccgctat
    15361 acggtgatga aagacggcag cagcgtttgc agcggcatgg tgagcgatgt gtcaaatgac
    15421 gatatcgtcc gtctgatggt aggccgcgaa ttgcaaaacc gttttaacgc gatgaaggag
    15481 aatgtcagca accttgcgca cgatacggtt tttgaggtgc gcaacgtcac cagccgtgac
    15541 agaaaaaagg tccgggatat ctcatttagc gtctgccggg gagaaatatt aggctttgcc
    15601 ggactggtcg gttccggacg tactgaactg atgaactgcc tgtttggcgt tgataaacgc
    15661 gctggcggag aaatccgtct taatggcaaa gatatctctc cacgttctcc cctggatgcc
    15721 gtgaaaaaag ggatggctta catcactgaa agccgccggg ataacggctt tttccccaac
    15781 ttttctatcg ctcagaacat agctatcagc cgcagtctga aagacggcgg ctataaaggc
    15841 gcgatggggt tgtttcatga agttgacgag caacgtaccg ctgaagccca gcgcgaactg
    15901 ctggcgctga aatgtcattc ggtaaaccag aacatcactg aactctccgg cggtaaccag
    15961 cagaaagtcc tgatctccaa atggctgtgc tgtcacccgg aagtgatcat tttcgatgaa
    16021 cctacccgtg gcatcgacgt tggcgcgaaa gccgaaattt acaaagtgat gcgccaactg
    16081 gcggacgacg gaaaagtcat cctgatggtg tcatctgaac tacctgaaat tatcaccgtc
    16141 tgcgaccgca tcgccgtgtt ctgcgaagga cgactgacgc aaatcctgac caatcgcgat
    16201 gacatgagcg aagaggagat tatggcatgg gctttaccac acgagtaaaa agcgaagcga
    16261 gcgagaagaa accgttcaac tttgcgctgt tctgggataa atacggcacc ttttttatcc
    16321 tggcgatcat cgtcgccatt tttggttcgc tgtcgtcaga atattttctg accaccaata
    16381 atattaccca gatttttgtg caaagctccg tgacggtact gatcggcatg ggggaattct
    16441 tcgctatcct ggtcgccggt atcgaccttt cggttggcgc gattctggcg ctttccggta
    16501 tggtgaccgc taaactgatg ttggcaggta ttgatccgtt tctcgcggcg ctaattggcg
    16561 gtgtactggt tggcggcgca ctgggggcga tcaacggctg cctggtcaac tggacgggac
    16621 tacacccgtt cattatcacc cttggcacca acgctatttt ccgtgggatc acgctggtga
    16681 tctccgatgc caactcggta tacggcttct catttgactt cgtgaacttc tttgccgcca
    16741 gcgtaattgg gatacctgtc cccgttatct tctcgctaat tgtcgcgctc atcctttggt
    16801 ttctgacaac gcgtatgcgg ctcgggcgca acatctacgc actgggcggc aacaaaaact
    16861 cggcgttcta ttccgggatt gacgtgaaat tccacatcct ggtggtgttt atcatctccg
    16921 gtgtttgtgc aggtctggca ggcgtcgtct caactgcacg actcggtgcc gcagaaccgc
    16981 ttgccggtat gggttttgaa acctatgcca ttgccagcgc catcattggc ggcaccagtt
    17041 tcttcggcgg caaggggcgc attttctctg tggtgattgg cgggttgatc atcggcacca
    17101 tcaacaacgg tctgaatatt ttgcaggtac aaacctatta ccaactggtg gtgatgggcg
    17161 gattaattat cgcggctgtc gcccttgacc gtcttatcag taagtaagga attgaacatg
    17221 aaaatctccc cctcgttaat gtgtatggat ctgctgaaat ttaaagaaca gatcgaattt
    17281 atcgacagcc atgccgatta ctttcacatc gatatcatgg acggccactt tgtgccgaat
    17341 ctgacgctct caccgttctt cgtaagccag gtaaaaaaac tggcgagtaa accgctcgac
    17401 tgccatctga tggtaacgcg tccgcaggat tacattgctc aactggcacg tgcgggagct
    17461 gatttcatca ctttgcatcc ggaaactatc aacggtcagg cattccgcct gattgatgaa
    17521 atccgccgtc atggcatgaa agtggggctg attcttaatc cggaaacgcc ggttgaggcc
    17581 atgaaatact atatccataa ggctgataaa attacggtca tgactgtcga tcccggtttt
    17641 gccggacaac ccttcattcc tgaaatgctg gataaagttg ccgaactgaa ggcatggcgt
    17701 gaacgagaag gtctggagta cgaaattgag gtggacggtt cctgcaacca ggcaacttac
    17761 gaaaaactaa tggcggcagg ggcggatgtc tttatcgtcg gcacttccgg cctgtttaat
    17821 catgcggaaa atatcgacga agcatggaga attatgaccg cgcagattct ggctgcaaaa
    17881 agcgaggtac agcctcatgc aaaaacagca taacgtcgta gcgggcgtgg atatgggggc
    17941 aacgcatatc cgcttttgtc tgcggacagc agaaggtgaa acgctacact gcgaaaaaaa
    18001 gcggaccgca gaagttattg ctcccgacct ggtgtcgggt atcggcgaaa tgattgacga
    18061 gcaactcagg cgctttaacg ctcgctgtca tggtctggtg atgggatttc cggcgctggt
    18121 cagtaaagat aaacgcacca ttatttctac gcctaacctg ccgttaacag cggcagattt
    18181 atatgatctc gccgataagc tcgaaaatac gctgaattgt ccggttgagt tttcccgcga
    18241 cgttaacctg caactctcct gggacgtagt agaaaatcgc cttacgcaac aactggttct
    18301 ggcagcctat ctcggtacgg ggatggggtt cgcagtgtgg atgaacggtg cgccgtggac
    18361 gggtgcacac ggtgtggcag gcgaactggg ccatatcccc ctgggagata tgacccaaca
    18421 ctgcgcgtgt ggtaatcctg ggtgcctgga aaccaattgc tctggaatgg cgctaagacg
    18481 ctggtacgaa caacagtccc gaaattaccc attgagcgat cttttcgtcc atgcggaaaa
    18541 cgcccctttc gtccagagtc tgcttgaaaa cgcggcacga gccattgcca ccagcattaa
    18601 tctgttcgat cccgatgcgg tgattctggg tggtggcgtg atggatatgc ccgccttccc
    18661 acgcgagact ctcattgcca tgacccaaaa gtacctgcgc cgtccactgc cgtatcaggt
    18721 cgtgcgcttt attgccgcct catcttctga ctttaatggc gctcagggtg cagcaatatt
    18781 ggcgcatcaa cgttttttgc cacagtcctg tgctaaagtc ccatgagtaa agcgttggca
    18841 cagtagcgca atgtcccgct gtgccaacga caggtacaat atatataata attacataaa
    18901 acagccaata ttattcccag caatgatagt tctactgtaa ttttccctct tcagcacaaa
    18961 tgcctaataa aatggctcgt tccgctcaca cttccactta ttaaagtcgc aatttcagaa
    19021 ttatgataac gccagcgtta aatgacgtat tttgcatgga ttacatattt acgacataaa
    19081 tgataaatgg agatttatat gaataactct cggttattcc gtttgagcag gattgttatt
    19141 gcgttcactg ccgccagcgg catgatgata aataccgctt acgcaacaaa tgaagcgaaa
    19201 gccgccactc aatataccca acaggttaat cagaattacg ccaaatcatt accgtttagc
    19261 aatcgtcagg attttgacga tgctcagcgt ggatttatcg ctccgctgct ggatgaaggt
    19321 attctgcgtg atgcgaacgg taaaatttac taccgcgcga atgattacaa atttgatatt
    19381 aatgccgctg cgccggaaac cgtaaacccc agcctgtggc gtcagtcgca gatcaacggc
    19441 atttcaggcc tgttcaaagt caccgataaa atgtatcagg tgcgcggcca ggatatctct
    19501 aacattacgt tcgttgaggg cgagaaaggc attattgtta tcgacccgct ggtgacgcca
    19561 cctgccgcaa aagccgcact tgacctttac ttccagcatc gtccgcaaaa accgattgtt
    19621 gccgttatct acactcacag ccacgccgac cactatggcg gcgtgaaagg tattatctct
    19681 gaagccgatg ttaaatccgg caaagttcag gtgattgctc cagctggctt tatggacgaa
    19741 gccatcagcg aaaacgtgct ggcgggtaac atcatgagcc gccgtgcgct ctactcttac
    19801 ggtctgttac tgccgcacaa cgcgcaaggc aacgtgggca acggcctggg cgtgacgctg
    19861 gcaaccggcg acccgagcat tattccaccg actaaaacta tcgtcagaac tggcgataag
    19921 atgattatcg acggcctgga atttgacttc ctgatgactc cgggtagcga agcgccagcc
    19981 gaaatgcact tctatattcc ggccctgaaa gccctgtgta ccgccgagaa cgccacgcat
    20041 accctgcaca acttctacac tctgcgcggc gcgaaaaccc gcgacaccag caagtggacc
    20101 gagtatctga acgaaacgtt ggatatgtgg ggtaacgacg cggaagtact gtttatgccg
    20161 cacacctggc cggtctgggg caataagcat atcaatgatt atattggtaa atatcgcgat
    20221 actatcaagt acattcacga ccagaccctg cacctggcga accagggcta caccatgaat
    20281 gaaatcggcg acatgattaa gctgccgcct gcgcttgcca ataactgggc cagccgcggc
    20341 tattacggtt ctgtcagcca caacgcccgc gcggtatata acttctatct tggctattac
    20401 gacggtaacc cggctaacct gcatccctat ggtcaggtgg agatgggtaa acgttacatg
    20461 caggcgctgg gcggttctgc ccgcgtcatc aacctggcga aagaagcgaa caagcaaggt
    20521 gattaccgct ggtcggcaga acttctgaaa caggtgattg ccgccaaccc gggtgaccag
    20581 gtcgcgaaga atctgcaagc gaataacttt gaacagctgg gctatcaggc cgagtccgcc
    20641 acatggcgcg gtttctacct gaccggcgcg aaagagctgc gcgaaggggt gcataagttc
    20701 agccacggca ccaccggttc cccggatacc attcgcggga tgtcggtcga aatgctgttc
    20761 gactttatgg ccgttcgact cgatagcgaa aaagctgccg ggaagaacat cagcctgaac
    20821 ttcaatatga gtaacggcga taacctcaac ctgaccctga acgatagcgt gcttaactac
    20881 cgtaaaacac tgcaaccgca agctgacgcc tctttctaca tcagccgtga agatctgcac
    20941 gccgtgctga ccggacaggc aaaaatggcc gatctggtga aagcgaagaa agccaaaatt
    21001 attggcaatg gcgcgaaact ggaagaaatt atcgcctgtc tggataattt cgatttgtgg
    21061 gtgaatatcg taaccccaaa ttaatgtcac tcgcttttgg gggcgctgaa gcctccaaaa
    21121 gcatttgct
//
LOCUS       DADSZD010000078        21036 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.078, whole genome shotgun sequence.
ACCESSION   DADSZD010000078 DADSZD010000000
VERSION     DADSZD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.078"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(142..1053)
                     /gene="gsiD"
                     /locus_tag="J1Z28_002536"
     CDS             complement(142..1053)
                     /gene="gsiD"
                     /locus_tag="J1Z28_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBA7717363.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLSMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1056..1976)
                     /gene="gsiC"
                     /locus_tag="J1Z28_002537"
     CDS             complement(1056..1976)
                     /gene="gsiC"
                     /locus_tag="J1Z28_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBA7717364.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1994..3532)
                     /gene="gsiB"
                     /locus_tag="J1Z28_002538"
     CDS             complement(1994..3532)
                     /gene="gsiB"
                     /locus_tag="J1Z28_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBA7717365.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFVGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3552..5423)
                     /gene="gsiA"
                     /locus_tag="J1Z28_002539"
     CDS             complement(3552..5423)
                     /gene="gsiA"
                     /locus_tag="J1Z28_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBA7717366.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVSRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAVVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5410..6375)
                     /gene="iaaA"
                     /locus_tag="J1Z28_002540"
     CDS             complement(5410..6375)
                     /gene="iaaA"
                     /locus_tag="J1Z28_002540"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA7717367.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6579..7814
                     /gene="moeA"
                     /locus_tag="J1Z28_002541"
     CDS             6579..7814
                     /gene="moeA"
                     /locus_tag="J1Z28_002541"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBA7717368.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEARSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7814..8563
                     /gene="moeB"
                     /locus_tag="J1Z28_002542"
     CDS             7814..8563
                     /gene="moeB"
                     /locus_tag="J1Z28_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBA7717369.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYHCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8639..9301)
                     /gene="fsa"
                     /locus_tag="J1Z28_002543"
     CDS             complement(8639..9301)
                     /gene="fsa"
                     /locus_tag="J1Z28_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBA7717370.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKSE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWQGAFGRT
                     SI"
     gene            9432..10331
                     /locus_tag="J1Z28_002544"
     CDS             9432..10331
                     /locus_tag="J1Z28_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBA7717371.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCELCAKTAPELIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMGLLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10337..12769
                     /locus_tag="J1Z28_002545"
     CDS             10337..12769
                     /locus_tag="J1Z28_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBA7717372.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            12915..13730
                     /gene="ybiV"
                     /locus_tag="J1Z28_002546"
     CDS             12915..13730
                     /gene="ybiV"
                     /locus_tag="J1Z28_002546"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBA7717373.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQAVLENKPPFNL"
     gene            complement(13930..15522)
                     /locus_tag="J1Z28_002547"
     CDS             complement(13930..15522)
                     /locus_tag="J1Z28_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBA7717374.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            15741..16661
                     /gene="ldtB"
                     /locus_tag="J1Z28_002548"
     CDS             15741..16661
                     /gene="ldtB"
                     /locus_tag="J1Z28_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA7717375.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(16719..17837)
                     /locus_tag="J1Z28_002549"
     CDS             complement(16719..17837)
                     /locus_tag="J1Z28_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBA7717376.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPESWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLSNVS
                     HLSDPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(17834..18301)
                     /locus_tag="J1Z28_002550"
     CDS             complement(17834..18301)
                     /locus_tag="J1Z28_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MntR"
                     /protein_id="HBA7717377.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            18487..18615
                     /gene="mntS"
                     /locus_tag="J1Z28_002551"
     CDS             18487..18615
                     /gene="mntS"
                     /locus_tag="J1Z28_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBA7717378.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            18887..20470
                     /locus_tag="J1Z28_002552"
     CDS             18887..20470
                     /locus_tag="J1Z28_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA7717379.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(20519..21034)
                     /gene="ompX"
                     /locus_tag="J1Z28_002553"
     CDS             complement(20519..21034)
                     /gene="ompX"
                     /locus_tag="J1Z28_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBA7717380.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
BASE COUNT         5186 a         5796 c         5198 g         4856 t
ORIGIN      
        1 gtgccgctgc aaaatagtcc cttgctgcgg ctttttaacc aaattacccg attctacaaa
       61 atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt gattaccctt
      121 tcaagcaata ttcaaacgta actatccttt aattttcgga tccagcgcat cgcgtaaacc
      181 atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg gaaaaacagc
      241 gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgccc cccactctgg
      301 tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga taatcgaggt
      361 gccaatgcgc atggtgaaaa acaccacgat agaagagacg gtcccaggca ggatatgacg
      421 caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa aggtttgctg
      481 tttcaacacc agcgtgttgc cgcgcaccag gcgggcaaac gcggggatgg aaaaaatggc
      541 gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag cgatcgccag
      601 taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgctcagcc gatcccacca
      661 gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac cgataaacac
      721 ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc tgaaaatgtc
      781 acgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat tcagattgtc
      841 ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa tagccaccac
      901 aatcaataaa ataacgaaca atgcggcggt catcgccata tgctgacggc gaaatcgtcg
      961 ccagaattca tgccacggtg tacgtacctg gtcaggtttg accagtggca tggcgtttaa
     1021 caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat agccgggtta
     1081 atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag cgagaaaagc
     1141 agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac gagtaagcgt
     1201 ccaagtccgg gccagttgaa aactttctcc accacgatgg aaccaccgag caaaaagcca
     1261 aactgtaaac ccatcatggt cactaccggg atcatcgcgt tacgtagccc gtgtttgagg
     1321 acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc ttcgcttaaa
     1381 acgtcgacaa acgacgcgcg ggtaaagcgc gccatcacgg cggccactgc cgcgccgagc
     1441 gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacggtagg cagccagccc
     1501 agttcaacag agaatacctg aattaaaagc atccccagag caaatgccgg aaacgaaatc
     1561 cccgacaccg caatagtcat actcaatcga tccggccaac ggttacgcca gacggcggcg
     1621 ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat ggtcagccac
     1681 agcgttggca taaagcggct ggcaatctca tcggcaaccg gacgacgcga caccatcgac
     1741 aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg atacagcggc
     1801 tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc ttcgggcccg
     1861 gcaatcaatc gcgccggatc gccgggcagc atgtggacaa ataaaaacac cagcaccgag
     1921 acgataaaca gcgtcggaat caaccccagt aagcgtttga taacgtaatt aagcattcca
     1981 ctccctgcgt tgcttattgc aaatccgcat cttcaaagct gaagccggtg tctggcatga
     2041 tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc accagcggga
     2101 tccacggcga ctcttgccag atgatatcct gcgccgcctt atacaagcgg gtcttttccg
     2161 ccggatcatt agttttcagt gcctgagcca ggaagtcatc cacctgttta ttgctgtaaa
     2221 acgcggtatt aaacagcgtt ggtggccagt tctgcgaggc aaacagcggc gatagagccc
     2281 agtcggcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg ccgctctctt
     2341 tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgca gtcacctggg
     2401 ctttaatccc aacctgcgct aactgctgct gggtaaattg cagcactttc tgcgcggtgc
     2461 tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc gcctctttca
     2521 gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc gcgtaggcga
     2581 tactcggcgg taccacacca gtagctggcg ttgcatagcc cgcgaaggcc actttcacca
     2641 gcgccgggcg gttaatggcg taattcagcg cctcacggac cttcgggtta tcgaacggct
     2701 tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc atcaactcga
     2761 tatttttgtt tttctccagc agtgcggcct gctcgtaggg aatggggaaa gcaaactgcg
     2821 cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga cgccaggtta
     2881 tgctgtccag tttgggcaat cctggctgcc agtaacccac gaattttttc accttcacaa
     2941 aatcggtctg attccaggta tccagttcat acggcccggt tcccaccgga tgaaaaccaa
     3001 tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc ggatgggcaa
     3061 gaatattaat aaacgctgag aatggctgtt tgagggtaat ctttaccgtt gtcggatcga
     3121 tcgcttccgt tttggcaata ttcttataca ggttatagcg tttaagatga ttcgccggat
     3181 cgctggcccg gtccagattc gctttcaccg ccgctgcgtt gaaatcggtg ccatcctgga
     3241 atttaatgcc ttcccgcaat ttcacggtgt aagtaaggcc gtcatcggaa acggtataac
     3301 tctccgccag cacgtttttc agtttcatct ctttatccag accgaacagc ccctggtaaa
     3361 acgatttcgc tacggcctga gataacgtgt catttgcgtc atacggatcg agcgtggtga
     3421 agttcgagcc caccgccacc accacatttt tggcagcgaa tgcacaagat gccatcaacg
     3481 ctgtcgcaat gcccagcgcc actaacccac tacggtgtac agctcttgcc attttattct
     3541 ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc gacgtaatgc
     3601 cccggcccga cgcattgcaa cgagacgact gcaacttctt cgccacgcag atgaatattg
     3661 ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg ttcagcgacc
     3721 ggaactgccg ccagtaattt acgcgtataa gggtgctgcg gattttcaaa gaccgcgcgc
     3781 cgtggaccaa tttcaacaat ttgcccgaga tacatcaccg ccacacgatg actaatccgc
     3841 tcaaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc acgctggaga
     3901 tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga gacggcttcg
     3961 tcggcaatga ttacttttgg attcaatgcc aacgcgcgag caatgcagat gcgctggcgc
     4021 tgaccgccgg aaaactcatg agggtaacgc caggcatgtt ccggtaacag acccacgcgc
     4081 tccagcaacc acgcaacgcg tgcagccgct tccttacctg gcaataaacc gtgtacgcgc
     4141 agcggttcga gaatcgaatc accgatggtc tgacgtgggt ccagcgaagc gtaagggtcc
     4201 tgaaaaataa actgaatatc gcggcgcaat gcctgaagtt taccgggtga caaggtatcg
     4261 attcgctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg cagcaacgcc
     4321 cgcccggtgg tggatttacc gctgccagac tcgcccacca gcgatagcgt ttcaccaggc
     4381 cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg attcaacaaa
     4441 ccgctgcgca aagggaaacg ggagaccaga ttacgcaccc gtaaaacagg ttcgccatcc
     4501 accaccgttt tctgctcgat gggtggctcc tgtttcgctg gatgttcaag cgatatcaat
     4561 gggaaacgtc ggggataatc taaccctttc atcgcaccaa gttgcggaac agcagctaac
     4621 agcgcacggg tataaggatg ttgcggtgca tgaaaaatct gttcgacgct acccgtttcc
     4681 accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac cacacccata
     4741 tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt aattaattgc
     4801 aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc aatcagcacc
     4861 gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc gccagagagt
     4921 tgatgcggat aacgtgaaag aatggtttgt gcctccggaa tgcgtacctg atccagcatc
     4981 cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg aatagattcg
     5041 gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc ctgaaatatc
     5101 atcgccatat ccgccccgcg cacatggcgc atttgtgcag cactctgctc gctaagttca
     5161 attacttcgc gactgcgccg ctgcaacagc attttatcgc actgcactaa accgcccgcc
     5221 tgttccaaca ggcgcatcaa tgccagcgca gtcactgact taccggagcc ggattcgcca
     5281 acaattgcca gcgtctcacc acgttgcagg ctaaaagaga gattgcggac cgcagctatt
     5341 ttctgctgct cctgcataaa ggcaatattc agattttcaa ccgccagcac atcaccggca
     5401 tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac cggtggttgg
     5461 cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg gtagcgcgac
     5521 attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag ggagtttttc
     5581 cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca ttaacgcggc
     5641 gatatcatat gccgccagcg cgcggatgaa gacttcgccc gtgccggtac aggaaaccgc
     5701 cacactggcg ttattggcat agcatccggc ccccactaag ggactatcgc caactcgtcc
     5761 gggtaattta ttggtcattc cgcccgtgga cgtggctgcc gccagattgc cgtctaaatc
     5821 caacgccacg gcccctacgg tgcccatttt ctgtttttca tccagtggcg caccgctatg
     5881 gtcgaggact gttgcccctt cctcgcgcgc tgccattagt tgttcataac gcaaaggtgt
     5941 ggagaaaatc tccggcgaga cgcgctccat gccatgagca aacgcaaaat tttctgcccc
     6001 ttcgccaatc atcatcacat gtgggctttg ctccatcacc agtcgggcgg caagaaccgg
     6061 attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac catccatcac
     6121 acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc cggcgttaaa
     6181 cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg cactttcgcc
     6241 cgctaccagc attttctgcc cggtttcaac aatggcagac aacgcctcga tgtagcgtaa
     6301 ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat gaattgcaat
     6361 gactgctttg cccatagtat tcgtccatta agggtggctc gccacttttg ctataaatat
     6421 cagaattttt atgaatgact cattcaattt ttgaatatag aaagatggat atggcatgta
     6481 aaggcaggag ccagcgctac gcccgaggtg gcatacagtc tgtggcgttt ttctgacata
     6541 ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc accggattga
     6601 tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt taccccactg accgcccagg
     6661 aaacgctgcc actggttcag tgttttggcc gtattctggc gagcgatgtc gtttcgccac
     6721 tggatgtccc ggggtttgat aactccgcta tggacggcta cgcggtgcgt ttagccgata
     6781 ttgcctccgg gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag ccgtaccatg
     6841 gtgaatggcc tgcgggtacc tgcattcgta ttatgaccgg tgcgccggtg ccggaaggct
     6901 gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg cgttttactg
     6961 ctgaagcgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct gcaggtgcgg
     7021 ttgttttccc ggcggggact cgcctgacta ccgctgaact gccagtgatt gcttcgctag
     7081 ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct accggtgatg
     7141 aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgatacc aaccgtctcg
     7201 ccgtacacct gatgttagaa cagttgggat gcgaggtaat caacttaggg attatccgcg
     7261 acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg gatgtagtga
     7321 tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt cttgaagagc
     7381 tgggggagat cgccttctgg aaactggcga ttaaaccggg taaaccgttc gcgttcggta
     7441 aactttctaa tagttggttc tgcggcctgc cgggcaaccc ggtttcagcg acgctgacct
     7501 tctatcaact ggtacagcct ttgctggcaa aactaagcgg caataccgcc agtggcctgc
     7561 ccgcgcgcca gcgtgtacgc acagcgtccc gcctgaaaaa aacgccagga cgccttgatt
     7621 tccagcgcgg cgtgctgcaa cgcaacgccg atggcgaact ggaagtgacg accaccggac
     7681 atcagggttc acatatattt agctccttta gcctcggcaa ctgctttatc gtgctggaac
     7741 gcgagcgcgg caatgttgac gtgggcgaat gggtggaagt cgaaccgttt aacgcgttgt
     7801 tcggaggcct gtaatggcag aactcagcga tcaggagatg ctgcgctaca accggcaaat
     7861 catcctgcgt ggttttgatt ttgacggtca ggaagcgctg aaggattctc gcgtgttggt
     7921 ggtggggctt ggtggcctcg gctgtgcggc atcgcaatat ctggcaagcg ccggtgtcgg
     7981 caacctgacg ctgctcgact tcgacacggt ttcgctctcc aatctgcaac gccagacgct
     8041 gcacagtgat gccacggtcg ggcaaccaaa ggtggaatcc gcccgtgacg ccctgacgcg
     8101 gatcaaccca catatcgcga ttacgccagt caatgcgctg cttgatgacg ctgaacttgc
     8161 tgcgatgatt gcaaaacacg atctggtgct cgactgtacg gataacgttg cggtacgtaa
     8221 tcaactgaac gcaggctgtt ttgccgcgaa ggtgccgctg gtttccggcg cggcaattcg
     8281 tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct atcactgcct
     8341 tagccgtttg tttggcgaga acgcgttaac ctgcgtagaa gcaggcgtga tggcaccgtt
     8401 aatcggcgta attggttcat tgcaggcaat ggaagcgatt aaactgctgg ccggttatgg
     8461 caaacctgcc agcggaaaaa tcgtgatgta cgatgcgatg acctgtcagt ttcgcgaaat
     8521 gaaactgatg cgtaatccgg ggtgtgaggt gtgcgggcag taactgccac ctccaggcca
     8581 gataagacgc ggcactccgc cacgtcatca gcaatttatg agaatgcaga acgtccggtt
     8641 aaatcgacgt tctgccaaac gctccttgcc agtcctgctc aaacttcgcc acagcggcat
     8701 caacagccgg atagctaatc atctgttgtg ccacatccag cggcagggtg attgactcac
     8761 accctgccag taagcagtcc agcgcctgac gcggggtttt gaaactcgcg gctaacactt
     8821 tcgcctgcgg cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc tgaataccgc
     8881 taccgccctg agcatcaata cgattaacgt aaggcgcaac atattccgca cctgccagcg
     8941 ccgacagtag cccttgtgct gcgccatata ccgcggttcc cagcgtcgga atcccttccg
     9001 attttaacat cttaatagct gccagcccct cagcggtcac cggaactttc accacgatat
     9061 ccgcaataat agaacgcaat ttacgtgcgt cattcaccat cccttcagca gtggtagcca
     9121 ttacctgggc aaacagacgc ccctgaccgc ccatcgcctc gtgaagttgc ggaagcacaa
     9181 cctccagcgg ttttttaccc gcagcgataa tgcttgggtt agtggttaca ccggccagcg
     9241 gaaaaatacg tgacagcgcc ttcaccgcaa caacgtctga agtatccaga tacagttcca
     9301 taaccatcct caaaatgtac tttttgtctg aatgtagact atcacgggaa aactctctga
     9361 acagttgacg tacatcaatc taactttcat tcgaaagtat tttaatgttt gaacgaaaca
     9421 gagaggcaac tatgattttc aatattcaac gctactcgac ccatgatggc cccggtatcc
     9481 gcacggtcgt atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa
     9541 gccgcgcccg cacgcaggat ctgctgtatg acccacgact ctgtctggaa ggctgtgagc
     9601 tgtgcgctaa gaccgcaccg gaattgattg agcgcgcgtt gaatggtttg cttattcatc
     9661 gggaaaagtt aaccccggag cattttacgg cgctaaccga ctgctgcccg acacaggcgt
     9721 taaccgtgtg tggtgaagtg aaaagcgttg aggagatcat ggcaaccgtt ttgcgcgata
     9781 aaccgtttta cgatcgcagc ggcggcggct taacactttc tggcggagaa ccgtttatgc
     9841 agccggaaat ggcgatgggg ctactgcaag ccagccacga agccggtatt catactgcgg
     9901 tagaaacctg tctgcatgtg ccgtggaaat atatcgcccc ttctctgccc tatatcgatc
     9961 tgtttcttgc cgatttaaaa cacgttgccg acgcgccgtt taaacagtgg accgacggta
    10021 acgccgccag ggtgctggat aacctgaaaa aactcgccgc agccggcaaa aaaatcatta
    10081 tccgcgtgcc actgattcaa ggctttaatg ccgatgaaac ttctgtgaaa gccattaccg
    10141 attttgccgc cgacgagctg cacgttagcg aaattcattt tctgccctac cacacactgg
    10201 gcatcaacaa atatcactta cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg
    10261 cgccagaact gctcgacttt gcccagcagt atgcctgcca gaaagggtta accgcgacct
    10321 tacgaggata acaaccatga caacactaaa actggatacg ctcagcgacc gcattaaagc
    10381 gcacaaaaat gcgctggtgc atattgtgaa accgccggtc tgtaccgaac gagcacaaca
    10441 ctataccgag atgtatcaac aacatctcga taagccaatc ccggtacgtc gcgcgctggc
    10501 actggcgcat cacctggcga atcgcaccat ctggatcaag cacgatgagt tgatcattgg
    10561 caaccaggca agcgaagttc gcgccgcgcc gatcttcccg gaatataccg tctcctggat
    10621 cgaaaaagag attgatgatc tggcagatcg tcccggtgct ggttttgcgg tgagcgaaga
    10681 gaacaaacgc gttctgcatg aagtgtgccc gtggtggcgc ggtcagaccg tacaggatcg
    10741 ctgctacggc atgtttaccg atgagcaaaa aggtctgctg gcgaccggca tcattaaagc
    10801 ggagggcaat atgacctccg gcgatgcgca cctggcggtc aatttcccgc tactgctgga
    10861 aaaaggtctt gatggtctgc gtgagaaagt ggcggaacgt cgctcgcgca tcaatctgac
    10921 ggttctggaa gatctgcacg gcgagcaatt cctgaaagcg attgatatcg tgctggtggc
    10981 agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt gaaatggccg cgaccgaaac
    11041 ccgcgaaagc cgtcgcgatg aactgctgac gatagcagaa aactgcgatc ttatcgccca
    11101 ccagccgccg cagactttct ggcaggcgct gcaattgtgt tacttcatcc agttgatttt
    11161 gcagatcgaa tctaacggtc actcggtatc gtttggtcgt atggaccagt atctctatcc
    11221 atactatcgc cgtgacgttg aactgaacca gacactggat cgcgaacacg ccatcgagat
    11281 gctacatagc tgctggttga aattgctgga agtgaacaaa atccgctccg gctcacactc
    11341 aaaagcctct gcgggaagtc cgctgtatca gaacgtcacc attggcgggc aaaatctggt
    11401 tgatggtcaa ccgatggacg cggtaaatcc actctcttat gcgatcctcg aatcttgcgg
    11461 tcgcctgcgt tcgactcagc ctaacctcag cgtgcgttac catgcgggta tgagtaacga
    11521 tttcctcgac gcctgtgtgc aggtgatccg ctgcggcttc gggatgccgg cgttcaacaa
    11581 cgatgaaatc gtcatcccgg aatttattaa actcggtatt gaaccgcagg acgcttacga
    11641 ctatgcagcg attggttgta tcgaaaccgc cgtcggtggc aaatggggct atcgctgtac
    11701 cggcatgagc tttatcaact tcgcccgcgt gatgctggcg gcgctggaag gcggtcgtga
    11761 tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg ttgtcagcgg gtaacttcaa
    11821 caacttcgat gaagtaatgg acgcatggga tacgcaaatc cgttactaca cgcgtaaatc
    11881 aatcgaaatc gaatatgtcg tcgataccat gctggaagag aacgtacacg atattctctg
    11941 ctcggcgctg gtcgatgact gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa
    12001 atatgactgg gtttctggcc tgcaggtcgg cattgctaac ctcggcaaca gcctggcggc
    12061 agtgaagaaa ctggtatttg aacagggcgc gattggtcag caacagctgg ctgccgcact
    12121 ggcggatgac ttcgacggtc tgactcacga gcagctgcgt cagcggctga ttaacggtgc
    12181 gccgaagtac ggcaacgacg atgatactgt cgatacgctg ctggctcgcg cttatcagac
    12241 ctatatcgac gaactaaaac agtaccataa cccgcgctat ggtcgtggtc cggttggcgg
    12301 caattattac gcgggtacgt catctatctc cgctaacgta ccctttggcg cgcagacgat
    12361 ggcaacgccg gacggacgta aagcgcatac cccgctggca gaaggcgcta gtccggcctc
    12421 cggtactgat catcttggcc ctactgcggt cattggctca gtgggtaaac tgcctacggc
    12481 cgcgattctc ggcggcgtgc tgctcaacca gaaactgaat ccagcaacgc tggagaacga
    12541 atctgacaag cagaaactga tgatactgct acgcaccttc tttgaagtgc ataaaggctg
    12601 gcatattcag tacaacatcg tttcccgcga aacgctgctg gaagcgaaaa aacatccgga
    12661 tcagtaccgc gatctggtag tgcgtgtcgc gggctattcc gccttcttca ccgcgctctc
    12721 tccagacgct caggacgata tcatcgcccg tactgaacat atgctgtaat ctcctcagcc
    12781 cggcgtcgtc gccgccgggc taaatcattc acatcatcaa tttcagcctc gctttcattc
    12841 gaaatataat ttgtgctctg cgtcacattg ttattacact gttacggttt cattttcgag
    12901 ccaggagcac acctatgagc gtaaaagtta tcgtcacaga catggacggt acttttctta
    12961 acgacgccaa aacgtacaac caaccacgtt ttatggcgca gtatcaggaa cttaaaaagc
    13021 gcggcattga gttcgttgtc gccagcggta atcagtatta ccagcttatt tcgttctttc
    13081 ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg
    13141 gcaagcagtt gttccacggc gaactgaccc gacatgaatc gcggattgtt attggcgagt
    13201 tgctaaaaga taagcaactc aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg
    13261 aaaatgcccc cgaagcattc gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg
    13321 taaaagatta tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg
    13381 aacaaatccc gttagtgatc gacaaactgc acatagcgct ggatggcatt atgaaacccg
    13441 tcaccagtgg ttttggcttt atcgacttga ttattcccgg tctacataaa gcaaacggta
    13501 tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg attggcgaca
    13561 gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg agcaatgctg
    13621 cggaaaacat taaacaaatc gcccgttacg ctaccgatga taataatcat gaaggcgcgc
    13681 tgaatgtgat tcaggcggtg ctggaaaata aaccgccgtt taatctttga ttatcgccaa
    13741 ttcagtttat tgagaaagtg catgtcatta tgccggatac ggcgtaaccg ccttatttgg
    13801 catacaaaat catgaaaatt caatatattg cgatacactg ccaggcctga taagcgtagc
    13861 gcaccaggca atcttgcgtt tgtcatcagt ttcagtagcc ctgaccatat cagggctact
    13921 gataataact tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc
    13981 gatcacgcgt tccggggtga tttccagaat acgggtcgcc agggagctta caaactcacg
    14041 gtcgtgagaa acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagcgactc
    14101 aatggattcc atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg
    14161 catcattaac ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttcgcagg
    14221 ctttttgatg tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc
    14281 gtcatcgcct tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcgaa
    14341 ctcatattca tggtcctggg cgtagtaacc aatgcgcgcg ttctcagacc attttacggt
    14401 gccgctgtcc ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc
    14461 gttggtaccc agtaccgcca gtttttcacc cacttccagc agcaggttga gatttttaaa
    14521 cagtggaccg ttatcaaacc ctttagtcag accttccact tccagcgcgt tacggaacag
    14581 tttcttatcc tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc
    14641 cagtttgatt ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt
    14701 ggcgctaaag cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt
    14761 atcagccagc agacgttcac gcgcctgagt cgccgccgtc atgtactcat cgtagttgcc
    14821 cggataaacg cgcagctcgc cgtaatccag atccgccatg tgggtacaaa ccatgttaag
    14881 gaagtgacgg tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc
    14941 cagccagcga atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatgtc
    15001 cggatcagca aacagcgcct gcgccagcag cacacgcagc ttccagccag gagcaacttc
    15061 actcatcggg ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccagc
    15121 gcgagcttcc gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt
    15181 atagccgtct tcttcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac
    15241 ttcccacaac tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc
    15301 aaactgatcc tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa
    15361 cgtcggctct aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc
    15421 gccaatcagg ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg
    15481 cttactgccg aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga
    15541 gatatgacaa accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag
    15601 gtctacactc aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag
    15661 ctggaatgaa cttataatgc gcttccagta ctctaatatt ctcaacccaa tggcctgcca
    15721 ggcacaaaat ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg
    15781 ctgttgtcgg cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga
    15841 gtcgcctggt tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg
    15901 agtattttgc cgccgagtac cagatgggac tttccaatat gatggaagcg aacccgggtg
    15961 tggatacctt cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc
    16021 cggataccgt tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc
    16081 cgaaagggac caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagaca
    16141 cgcctatcaa ctggaccacc aaagttgagc gtaaaaaagc aggcccgacc tggacgccga
    16201 ccgccaaaat gcacgcagag taccgcgctg cgggtgaacc tcttccggcg gtcgttccgg
    16261 ctggtccgga taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc
    16321 atggcaccaa cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc
    16381 gtaacgaaga catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta
    16441 ttgatgagcc ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata
    16501 acccgctgtc taccactgaa gcccagtttg aaggtcagga aattgtgcca attaccctga
    16561 cgaagagcgt gcagacagtg accggtcagc cagatgttga ccaggttgtt ctggatgaag
    16621 cgatcaaaaa tcgttccggg atgccggttc gtctgaatta atcttcaaaa cgtaaagcta
    16681 aggcggactc ataatccgcc tttttaattt gacagacctt atcccgccgg gatcataacc
    16741 aacaaaacat atcccaccag cgccgcccac aacagcatcg gtatcgaata aagatgaaaa
    16801 cgccaccaga tacggcgatc gttcgccatc cgtaacgcaa tcaaatttgc cagcgatccg
    16861 ggtaataaac caaagccacc tacgtttacc gcccatgcca gtaataaaga cggtggcaca
    16921 taattgagca gcaaaatggt actcggcacg ttactgatca cctgcgataa accaatcgcc
    16981 gttaaccata atccggggtc ggataaatga ctcacgttac ttaacacccc ctgcaaagct
    17041 ggcagttggg ttagtaaatg gacgtcgata aacatcgcca taaacaccag cagcagcgtc
    17101 cagtccacac tgagcaccac gcgacgcgcc agcagcgcaa agcccgccgc cacaatcacc
    17161 agtccccaca gctcttgttt taactccagc gccgtcagaa agacgatata cagccccaga
    17221 caactccaca ccagccgcgg tttccactcc ggcgtttgca cccccgtatg gtattgcagc
    17281 gcctttccag ggaaacaaca ccagcacaac agcaggagtg tcagcatcat tgcgccagcc
    17341 agcggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc ccagataaga
    17401 atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc cagcgcctca
    17461 aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat agtcagcgga
    17521 acaactataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc tgccgccagt
    17581 accataaaca tcgccagccg acgctccgta gcaaaacggc gcaccatttt gcgccccagc
    17641 acatcaaaat aaccgcttaa ctcgacacct ttggtcagca gcatcaggcc gcttaaggtg
    17701 atgatggtgt gccagtcgat agcagcaggc caggattccg gtgcaaaggg aacgaaaaaa
    17761 cttaatccga tgccaacaag aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt
    17821 aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaag gcatccagcg
    17881 tctcttcact aacatggtgc tccatacctt ccgcgtcgcg acgggcgatt tccggactga
    17941 cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca
    18001 gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg gatcatttca atcagcccca
    18061 tggttgccag tcgcttaagc attttagcca ccgtcggttg cgaaaccccc agacgagcag
    18121 ccatgtctac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc agctcaacgt
    18181 aatcatcaat cagctcgcgc cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct
    18241 cttccacgtt cactaactgc gtcacttttt ttgctgttgg cgtacctgcg cgacgactca
    18301 ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg cacattgtaa
    18361 accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctatata atatagcctg
    18421 tgctatatct gtatgtaatg caatcatccc tcaaggatcg atgggattag caagtcagga
    18481 ggtcttatga atgagttcaa gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta
    18541 cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca gcaggataaa
    18601 ccttccgata aatagcggcg tcgcggtacg ccgcttcact cctgctttta tgcaggcaca
    18661 acgcgttttg gtctgaaaaa ccccactttt tgtcggattt gcaatcccct tcgcaaaaga
    18721 tttgttcgtc ggtagttgac ctgaacagca gctcgctcta tcttcttgca gccctgcgta
    18781 tattgcggct cgcggatgcg gacccctttc ccctcttcac gcactcttgc aggtattgac
    18841 ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga atttaaccct
    18901 caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt tctacttttt
    18961 acagtcgcta ttaattaacc tcggcttagg ttaccccttc agtttgctct acaccgctgc
    19021 gtttacggct attttgcttt tgctatggcg aacattgcct cgcgtacaaa aagttctggt
    19081 cggtatcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct acggcgcgcc
    19141 taacttcaat acattgctgg cattgcactc caccaatatg gaagagtcga ccgaaatcct
    19201 gacgattttt ccatggtaca gctacctggt cggcatattt atttttgcgc tcggcgtaat
    19261 agcgatcagg cgaaaaaaag agagtgaaaa agcgcgctgg aataccttcg acagcctgtg
    19321 cctggtattc agtgtggcga cattttttgt tgctcccgtg caaaacctgg cctggggcgg
    19381 cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt
    19441 caataataac gaggtgattg aagagcaaga acggatggca aaactttccg gaatgaaaga
    19501 tacctggacg gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg tgatcggaga
    19561 aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata ccccgtttgc
    19621 cagcagcgtt aacggtttga tatttgctga ctacattgcc gccagtggct ccacgcagaa
    19681 atcgcttggc ttaacgctca atcgcgttgt cgatggcaaa ccacagtttc aggataactt
    19741 tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc agggccaaat
    19801 cggcgaatac gataccgcca tcgccagcat cgccaaacga gcagatgaag tgtacttcct
    19861 gaaagaaggt aattttgaag cagataaaaa cacgaaagac gaagcgttac tggatatgac
    19921 cgctcaagtg ctggcgcaag agcactcgca accgcagctg attgttctac atctgatggg
    19981 ctcgcatccg caggcctgcg accggacaca aggaaaatac gaaacctttg tgcaatcgaa
    20041 agaaacgtcg tgctatctct ataccatgac gcaaacagac gatttactgc gcaagctgta
    20101 cgatcagtta cgcaacagcg gcagcagctt ctcgctggtt tacttttctg accacggtct
    20161 ggcctttaaa gagcgcggca aagacgtgca ataccttgct catgatgata agtatcagca
    20221 aaatttccag gtgcctttta tggtcatttc cagcgacgat aaagcgcatc gggtgattaa
    20281 agcccgccgc tcggcgaatg acttcttagg ctttttctcg cagtggacgg gaattaaagc
    20341 gaaggaaata aatatcaaat acccgtttat atctgagaag aaagccgggc cgatatacat
    20401 caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata ttttcgaccc
    20461 gaaaccttaa aacaaaaaaa tccgccccga gaggcggatt tttatatcac cgaagtgatt
    20521 agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga atacggctct
    20581 gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga ccagcaccgt
    20641 aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg gtctggaatt
    20701 taccataacc cacacccact acaccgtaga tgcttgccca gtcgttaatg cggtaagccg
    20761 gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt gcagtacggc
    20821 ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct tcttcatagc
    20881 ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg ctctgtgcgt
    20941 aaccgccagt tacagtagaa gtcgcagcta cggaagtacc tgcggtgaaa gccagaactg
    21001 cggccagtgc tgaaagacat gcaatttttt tcataa
//
LOCUS       DADSZD010000079        20340 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.079, whole genome shotgun sequence.
ACCESSION   DADSZD010000079 DADSZD010000000
VERSION     DADSZD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.079"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            577..678
                     /locus_tag="J1Z28_002554"
     CDS             577..678
                     /locus_tag="J1Z28_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717381.1"
                     /translation="MRTHNPNSLLPSQMQKCTCVFLHPASDLCGGEA"
     gene            675..1130
                     /gene="ybcN"
                     /locus_tag="J1Z28_002555"
     CDS             675..1130
                     /gene="ybcN"
                     /locus_tag="J1Z28_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HBA7717382.1"
                     /translation="MNLPQDGIKLHRGNFIAIGQQIQPYLENGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKSFATAAWVKDALKHTYLGYETKDLVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            1130..1300
                     /locus_tag="J1Z28_002556"
     CDS             1130..1300
                     /locus_tag="J1Z28_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717383.1"
                     /translation="MATPLIRVMNGHIYRVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1293..1583
                     /locus_tag="J1Z28_002557"
     CDS             1293..1583
                     /locus_tag="J1Z28_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBA7717384.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIKA"
     gene            1580..1942
                     /locus_tag="J1Z28_002558"
     CDS             1580..1942
                     /locus_tag="J1Z28_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBA7717385.1"
                     /translation="MNTYCITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVARIIKN
                     AMLDIGLAIPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            1939..2079
                     /locus_tag="J1Z28_002559"
     CDS             1939..2079
                     /locus_tag="J1Z28_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBA7717386.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPSSVLTDYRILKNYAKTLKGA
                     AA"
     gene            2076..2765
                     /locus_tag="J1Z28_002560"
     CDS             2076..2765
                     /locus_tag="J1Z28_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA7717387.1"
                     /translation="MNTQYLQYVREQLIVATADLSGATKGQLEAWLEHAQFDTGTYKR
                     KKPRILDEVTGRMITLDNPPISGKQLYAKGSSIALVSQVEFSTSSWRRAVLSLEEHQK
                     AWLLWSYSESVRWEHQVTITQWAWSEFKTLLGTRKIAGKTLERLKKLIWLAAQDVKNE
                     LAGRKTYEYQELASLVGVTSKNWSETFTERWVAMKHIFLQLDSQALLLLTKTRSKQKT
                     IFSQQDIAKLD"
     gene            3087..3392
                     /locus_tag="J1Z28_002561"
     CDS             3087..3392
                     /locus_tag="J1Z28_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA7717388.1"
                     /translation="MHEKESLAGAFWLVLLIIAGWGGLVRYLIDVKQSKATWSWINAL
                     AQIVVSGFTGVIGGLISIESGFSIYMILATAGISGAMGSVALTYFWERLTGVKNAKS"
     gene            3379..3855
                     /locus_tag="J1Z28_002562"
     CDS             3379..3855
                     /locus_tag="J1Z28_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBA7717389.1"
                     /translation="MQNLNPQRKAFLDMLAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLHPKLKSIAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTLRESEV"
     gene            4072..4254
                     /gene="rzoD"
                     /locus_tag="J1Z28_002563"
     CDS             4072..4254
                     /gene="rzoD"
                     /locus_tag="J1Z28_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HBA7717390.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(4345..4638)
                     /gene="iss"
                     /locus_tag="J1Z28_002564"
     CDS             complement(4345..4638)
                     /gene="iss"
                     /locus_tag="J1Z28_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HBA7717391.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            5119..5445
                     /locus_tag="J1Z28_002565"
     CDS             5119..5445
                     /locus_tag="J1Z28_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HBA7717392.1"
                     /translation="MKRRLLLLFLLSVLAVGCSQQKADEPRQLVTVYPRYPEYAAANY
                     IKGLVEVKFDIGADGTVTRIVFLRSEPHNLFRDEVVKAMAKWRFEKNRPCQGVKRQFI
                     FTPSRP"
     gene            5652..5834
                     /locus_tag="J1Z28_002566"
     CDS             5652..5834
                     /locus_tag="J1Z28_002566"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717393.1"
                     /translation="MLAVFQLVKNTCSRLLQCLQHAAAMRLAFTNHLSFLYQSLRELI
                     MEYQVNADVIGFGYAA"
     gene            6396..6941
                     /locus_tag="J1Z28_002567"
     CDS             6396..6941
                     /locus_tag="J1Z28_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBA7717394.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     MAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            6916..8841
                     /locus_tag="J1Z28_002568"
     CDS             6916..8841
                     /locus_tag="J1Z28_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA7717395.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPAIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRDFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESLHFMRFHVACPHCGEEQYLKFGDKETLFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYLAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPNIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            8838..9044
                     /locus_tag="J1Z28_002569"
     CDS             8838..9044
                     /locus_tag="J1Z28_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717396.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRAEFTATSVSDLK
                     KYIAELEVQTCMTQRRRGPAGFYV"
     gene            9041..10642
                     /locus_tag="J1Z28_002570"
     CDS             9041..10642
                     /locus_tag="J1Z28_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7717397.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSMARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     TWAAAAFESGLRQSTEEEKSDSRAA"
     gene            10623..11942
                     /locus_tag="J1Z28_002571"
     CDS             10623..11942
                     /locus_tag="J1Z28_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBA7717398.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     DGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            11952..12284
                     /locus_tag="J1Z28_002572"
     CDS             11952..12284
                     /locus_tag="J1Z28_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA7717399.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVDADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            12340..13365
                     /locus_tag="J1Z28_002573"
     CDS             12340..13365
                     /locus_tag="J1Z28_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA7717400.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIILQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            13407..13802
                     /locus_tag="J1Z28_002574"
     CDS             13407..13802
                     /locus_tag="J1Z28_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBA7717401.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLSGTAFRVSAGVAAEMTERGLARMQ"
     gene            13814..14167
                     /locus_tag="J1Z28_002575"
     CDS             13814..14167
                     /locus_tag="J1Z28_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717402.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            14179..14757
                     /locus_tag="J1Z28_002576"
     CDS             14179..14757
                     /locus_tag="J1Z28_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717403.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPCAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALHHQLRMVIKR"
     gene            14754..15149
                     /locus_tag="J1Z28_002577"
     CDS             14754..15149
                     /locus_tag="J1Z28_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717404.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEEDFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            15157..15897
                     /locus_tag="J1Z28_002578"
     CDS             15157..15897
                     /locus_tag="J1Z28_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717405.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASSSVVKGQGTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            15913..16335
                     /locus_tag="J1Z28_002579"
     CDS             15913..16335
                     /locus_tag="J1Z28_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA7717406.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPQKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGSAEPVSAGKCSTVS"
     gene            16317..16751
                     /locus_tag="J1Z28_002580"
     CDS             16317..16751
                     /locus_tag="J1Z28_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA7717407.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            16744..19305
                     /locus_tag="J1Z28_002581"
     CDS             16744..19305
                     /locus_tag="J1Z28_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7717408.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLVGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDETRARYWDDREKARLALEAARKKTEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKGYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGSWMAGLRSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GKFEQNNHVVINNDGTNGQIGPQALKAVYDVAHKAAMDVVTGQMRDGGLFSGGGR"
     gene            19302..19631
                     /locus_tag="J1Z28_002582"
     CDS             19302..19631
                     /locus_tag="J1Z28_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717409.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            19631..20329
                     /locus_tag="J1Z28_002583"
     CDS             19631..20329
                     /locus_tag="J1Z28_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA7717410.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
BASE COUNT         4896 a         4969 c         5986 g         4489 t
ORIGIN      
        1 actgacatgt gaagctgtct gacagaacga gttcctttta tctgggaata aacaacatca
       61 ccaatgagat cgacctggtt tctggtcggt gaaacactaa tgatatctgc acccggggtg
      121 taaccaggaa gattagtctg gacaggtgtt gcacaactcc cgaccgacat ggccattgac
      181 ataccataca atagtgttgc agatgataat atcttgttaa ttttcatgtt ttacatgcct
      241 tgacttatcg gatgaaattc tcttactgaa tttaacggca gtttaatagc cgtacaactc
      301 gtggtagcca gggaaatatc ccagtcgtgg ttaaggttgt acttgacggc tattatttca
      361 acaatgctta aagtgagagc ttaaggttat gcgtatgttt tacagtccga gtagattctg
      421 accaatagct tctttgtata attctgtcag aaataaatct tggcttgcat gaagtttgag
      481 accttatctt gtttgattat gaataatcaa ttcgccataa ttgtatcacc ggagcctgaa
      541 caactccggt gacttctgcg ctaaacgggg acgtttatgc gcacacacaa tccaaactct
      601 cttctccctt cacagatgca gaaatgcacc tgcgtttttt tgcatccagc gtctgacctc
      661 tgtggaggtg aagcgtgaac ctaccacaag atggcatcaa actgcatcgc ggtaacttca
      721 tcgctatcgg ccagcagatc cagccttatc tggagaacgg aaaatgcttt cgcatggtgc
      781 ttaaaccgtg gcgtgagaaa cgcagtcttt cccagaatgc actcagccac atgtggtaca
      841 gcgaaatcag tgaatacctc atcagcaggg ggaaatcgtt cgctaccgca gcatgggtaa
      901 aagatgctct caaacacaca tacctcggtt atgaaaccaa ggacctggtt gatgtcgtaa
      961 ccggcgaaat cactactatc cagtcgttac gccatacctc cgatctcgat accggagaga
     1021 tgtatgtctt cctgtgtaag gttgaagcct gggcggtgaa tattggctgc cacctgacta
     1081 ttccgcagag ctgcgagttc cagctgctgc gcgacaagca ggaggcgtaa tggctacacc
     1141 gcttattcgt gtcatgaacg gacacatcta cagagtacca aatcgtcgta agcgtaagcc
     1201 tgagctgaaa ccatccgaaa taccaacact gctcggatat accgccagcc tggttgataa
     1261 aaaatggttg cgactggcag caaggaggaa tcatggctga cttgagaaaa gcagcgcgtg
     1321 gtcgggaatg ccaggtaaga atccctggcg tatgtaatgg caatcctgaa acgtcagtac
     1381 tggcacatat ccggctggct ggattgtgcg gtaccggtat caaaccgcca gacctgattg
     1441 ccaccattgc atgttctgcc tgccacgacg aaatcgaccg tcgcacgcat tttgttgacg
     1501 ctggatatgc aaaagaatgt gcgctggaag gtatggcgag aacacaggtt atctggctga
     1561 aagagggggt aattaaggcg tgaatactta ctgcatcaca ctaccctggc cgccgagcaa
     1621 taatcgctat taccgccata atcgcggacg cacgcatatc agcgcagagg ggcaggcata
     1681 ccgcgataat gtcgcccgaa tcattaaaaa cgcaatgctg gatatcggcc tggctattcc
     1741 tgtgaaaatc agcattgagt gccacatgcc ggatcgccgt cgccgtgacc ttgataatct
     1801 gcaaaaggcc gcttttgacg cactcaccaa agcaggtttc tggctggatg atgctcaggt
     1861 cgttgattac cgcgttgtga agatgcccgt taccaaaggt gggaagctgg agctgactat
     1921 cactgaactg ggagatgaat gatgtttgag tcttatatgg cagaacgtct tcgccgccgc
     1981 tgggtgcgcc tgcgcttata tcgttttcct agttctgttt tgaccgatta ccggatactg
     2041 aagaattacg ccaaaacact gaaaggagct gccgcatgaa tacccaatat ttacagtatg
     2101 tccgcgagca actcattgtg gctaccgctg atttgagcgg agcaacgaaa ggacagcttg
     2161 aagcctggct ggagcatgca caatttgata ctggtacata caaacgaaag aagccgcgca
     2221 ttctggatga ggtaactggc aggatgatta cgctggataa tccgccgatt tccggtaagc
     2281 agttgtacgc aaaaggttca tccattgcac tggtcagcca ggttgagttc tcaacctcgt
     2341 catggcgccg cgcggttctg tctctcgaag aacatcagaa agcgtggttg ctgtggagtt
     2401 acagcgaaag tgttcgctgg gaacatcagg tcaccataac gcagtgggca tggagcgagt
     2461 ttaagactct gttgggtacc aggaaaattg caggtaaaac actggaacgt ttgaagaagt
     2521 tgatctggct ggcggcacag gatgtgaaga acgagctggc agggcgtaag acctatgaat
     2581 accaggagct ggcatcactg gtgggagtga catcaaaaaa ctggtctgag acatttactg
     2641 aacgctgggt tgcaatgaag cacatttttc tacagcttga tagccaagct ttattgcttt
     2701 taacgaaaac acgttcaaaa caaaagacca tattttcaca gcaagatatt gcaaaactgg
     2761 attaaaaatc atatatttta tgtaaatctg atattttgcc aatgttgtac gcactggcag
     2821 taatccaaat tcaagcccga ggtttaaagc cttgggcttt tctgtttctg aacggttagt
     2881 agccttccaa cctaccccag ccagggtgtc ttcagctgtt gagttgatat tgcttaaccc
     2941 tctgttgcca gctacatgct ggctttttta ttccaggctt gcggggagca tcaactccgt
     3001 gctttgtcgt taaattaccc cgtgagcctg atttctgaca tttaacgtcc cggccttttg
     3061 tcggcggcga aacattggct attcatatgc acgaaaaaga gagccttgcc ggagcgttct
     3121 ggctcgtttt gctgatcatc gcaggttggg gcggtctggt ccgctacctg atagatgtga
     3181 agcagagtaa agcaacgtgg agttggataa atgctctggc tcaaatagtg gtatcaggat
     3241 tcaccggtgt tattggtggc ctgatcagca tcgaaagtgg attcagtatt tacatgattc
     3301 tcgcgacagc ggggattagt ggtgcgatgg gttcggttgc actgacgtac ttctgggaac
     3361 gactgacagg ggtgaaaaat gcaaaatctt aatcctcagc gtaaagcctt cctcgatatg
     3421 ttggcgtggt cagaaggaac ggataacggg cgacaaccga cacgtaacca cggttatgat
     3481 gttattgttg gcggcgaact gtttactgat tactccgatc accctcgcaa acttgtcacg
     3541 ctacacccca aactcaaatc aatagccgcc ggacgttatc agcttctttc acgctggtgg
     3601 gatgcttacc gtaaacagct tggtttgaaa gacttctccc ccaaaagcca ggacgcagtg
     3661 gcattacagc agattaaaga gcgtggtgct ttaccgatga ttgatcgcgg tgatatccgt
     3721 caggcaatcg accgttgcag caatatctgg gcttcactgc cgggcgctgg ttatggtcag
     3781 ttcgagcata aggctgacag cctgattgca aaattcaaag aagctggcgg aacgctcaga
     3841 gagagtgagg tatgagcaga ataaccgcga ttatctccgc tctggttatc tgcgtcatcg
     3901 tctgcctttc atgggcggtt aatcattacc gcgataacgc gattacctac aaagcccagc
     3961 gcgacaaaaa tgccagagaa ctgacgctgg cgaacgcggc aattactgac atacagatgc
     4021 gtcagcgtga tgttgctgcg ctcgatgcaa aatacacgaa ggagttagct gatgcgaaag
     4081 ctgaaaatga tgctctgcgt gatgatgttg ccgctggtcg tcgtcggttg cacatcaaag
     4141 cagtctgtca gtcagtgcgt gaagccacca ccgcctccgg cgtggataat gcagcctccc
     4201 cccgactggc agacaccgct gaacgggatt atttcaccct cagagagagg ctgatcacta
     4261 tgcaaaaaca actggaagga acccagaagt atattaatga gcagtgcaga tagagctgcc
     4321 catatcgatg ggcaactcat gcaattattg tgagcaatac acacgcgctt ccagcggagt
     4381 ataaatgcct aaagtaataa aaccgagcaa tccatttacg aatgtttgct gggtttctgt
     4441 tttaacaaca ttttctgcac caccacaaat ttttgctgca tcgacagttt tcttctgccc
     4501 aattcccgaa acgaagaaat gatgggtgat ggtttccttt ggtgttactg ctgtaggttt
     4561 gtttccaaca gtaaacgtct gttgagcaca tcctgtaata agcattgcca gagcggcaga
     4621 aaacaacatt tttttcatct tattatcctg cattgttaaa aacggcagaa tcctatgtga
     4681 caacaattaa acgatagtta aatggattga tgaaaattaa aactacacag gtgggctcag
     4741 actattggag gaagttgggg acactcagaa tcctgtggaa tgaaataaac cggtctatcc
     4801 gtccattacc cttttagctg cgctgtatcg tcgccgtatt cccgcattaa ccatgaccgt
     4861 agcccgacgg ggaattcctt ctgcgtgagt gtgcgggaat aattaaaaac gatgcacacc
     4921 gggtttttac cgcgttaatg attcgcgggt ttatcccggt gcgatggtgg aagaaacagg
     4981 aagctgtatt acagaaagtg ctactactgt atcccgatgc gatgtatgta atgtgagtca
     5041 gataatggca caggatgtgg tgatgtggca gtctggaaca caggatatat tgtcagaata
     5101 agacccgtag gaataaaaat gaaaagacgc cttttactac tttttctgtt atctgtcctg
     5161 gcagtgggat gctcgcagca aaaagctgat gagccccggc aattagtaac ggtgtatcca
     5221 cgatatccgg aatatgctgc agcaaattat atcaaggggc tggttgaggt taagttcgat
     5281 attggtgctg atgggactgt gacacggatc gtttttctcc gctcagagcc tcataatttg
     5341 tttcgtgatg aagtggtgaa ggccatggcg aaatggcgat ttgaaaagaa tcgcccctgt
     5401 cagggagtga agagacaatt tatctttacg ccgtcacgtc cttgatgctt ccaggtagag
     5461 aggggctgga agcaggagaa aaatgaaaga gccagcggtt atatttttgt catggctgac
     5521 gaggaatgat ggaagaaggc gttgtatgcc acacaacgcc tcactgttca tttcttcttt
     5581 ttctctggtg gaacccgatg aataagagtt gtactggttt ccgatgagat ggcgatatac
     5641 tcgggcaaag tatgctggca gttttccaac tggtcaaaaa tacctgctct cgtctgttgc
     5701 aatgcctgca gcatgcggcg gcaatgcgcc ttgcttttac taaccatctt tccttcctct
     5761 atcagtcgct gcgtgaactc atcatggaat accaggtaaa tgcggatgtt atcggttttg
     5821 gctacgcagc atagtacaaa acggacaggt gcatcccggg acgggggagg cgtcacatgt
     5881 ccctgtgatg gttgttccgg gtaatgtact gtgtggggca taaaaatgtc cgataatttt
     5941 actttctacc gcagttagtt gattcgttgg tcctggtagc acattgggcg aggatttaaa
     6001 tgccaggcaa ctgaaggatg atgtcgcaag ggagatagcg agaatatttc tgattttcat
     6061 ttgatgatgc ctctgtgtga aatgacggta aacgacgcac ttgtgccggc acataatagc
     6121 aagcaccata atagatcaga ttcgattctt gctgtaagtg ataattattc tcgttttcgg
     6181 gtcctttccg tcgatccaac aggttacggg gcggcgacct cgcgggtttt cgctatttat
     6241 gaaaattttc cggggaaaat catgtcggta cttctcgaac ataactattt gttttttcta
     6301 atatcgaatc cgtaaaggtc cgacatgaaa acgcctaaaa aagtcatttt cgggcacttt
     6361 catgtcggac cctgtattta ttgtgagact gtttcatgaa ggttaataaa aagaaacttg
     6421 ccgaaatttt caacgtggat ccgcgaacga ttgaacgctg gcagtctcag ggactccctt
     6481 gcgtctccgg aggtggtaag ggcgttgaat ctgtatttga taccgccatg gcaattcagt
     6541 ggtatgcgca gagggaagct gatatcgaaa atgaaaaact ccgtaaagag gttgaggatt
     6601 acagggctgc cagcgaggca gatctccagc ctgggactat tgagtacgaa cgccatcgac
     6661 ttacgcgtgc gcaggctgac gcccaggagc tgaagaatgc cagagactcc gcagaagtgg
     6721 tggaaaccgc attctgtact ttcgtgctgt cacggatcgc aggtgaaatt gccagtattc
     6781 ttgacgggat ccctctctcg gtacagcggc gttttccgga actggaaaac cgacatgttg
     6841 atttcctgaa acgggatatc atcaaagcca tgaacaaagc agccgcgctg gatgaactga
     6901 taccggggtt gctgagtgaa tatatcgaac agtcagattg atattctgcg gcgtgatgta
     6961 cgcgccgggc tgcgagccct gttcaggccg gagccacaga ccgccgttga atgggcggat
     7021 gccaattact atctcccgaa agaatccgca taccaggaag ggcgctggga aacactgccc
     7081 tttcagcggg ccatcatgaa tgcgatgggc agcgactaca tccgcgaggt gaatgtggtg
     7141 aagtctgccc gtgttggtta ttccaaaatg ctgttgggtg tttatgccta cttcatagag
     7201 cataagcagc gcaacacact tatctggttg ccgacggatg gtgatgccga gaactttatg
     7261 aaaactcacg ttgagccggc catccgtgat attccttcgc tgctggcgct ggccccgtgg
     7321 tatggcaaaa agcaccggga taacacgctc accatgaagc gtttcaccaa tggtcgtgac
     7381 ttctggtgcc tgggcggtaa agcggcaaaa aactaccgtg aaaagtcggt ggatgtggcg
     7441 ggttatgatg aacttgctgc ttttgatgat gatattgaac aggaaggctc tccgacgttc
     7501 ctgggtgaca agcgtattga aggctcggtc tggccaaagt ccatccgtgg ctccacgccc
     7561 aaagtgagag gcacctgtca gattgagcgt gcagccagtg aatccctgca ttttatgcgt
     7621 tttcatgttg cctgcccgca ctgcggggag gagcagtacc ttaaatttgg tgataaagag
     7681 acgctgtttg gcctcaaatg gacgccggat gatccctcca gcgtgtttta tctctgcgag
     7741 cataatgcct gcgtcatccg tcagcaggag ctggacttta ctgatgcccg ttatatctgc
     7801 gaaaagaccg ggatctggac ccgtgatggc attctctggt tttcgtcatc cggtgaagag
     7861 attgagccgc cggacagtgt gacctttcac atctggacag cgtacagccc gttcaccacc
     7921 tgggtgcaga ttgtcaaaga ctggatgaaa acgaaagggg atacgggaaa acgtaaaacc
     7981 ttcgtgaaca ccacgctcgg tgagacgtgg gaagcgaaaa tcggcgaacg tccggatgct
     8041 gaagtgatgg cagagcggaa agagcattat ttagcgcccg ttcctgaccg tgtggcttac
     8101 ctgaccgccg gtatcgactc ccaactggac cgctacgaaa tgcgcgtatg gggatggggg
     8161 ccgggtgagg aaagctggct gattgaccgg cagattatta tgggccgcca cgacgatgaa
     8221 cagacgctgc tgcgtgtgga tgaggccatc aataaaacct atacccgccg gaatggtgca
     8281 gaaatgtcga tatcccgtat ctgctgggat attggcggga ttgacccgac cattgtgtat
     8341 gaacgctcga aaaaacatgg gctgttccgg gtgatcccca ttaaaggggc atccgtctac
     8401 ggaaagccgg tggccagcat gccacgtaag cgaaacaaaa acggggttta ccttaccgaa
     8461 atcggtacgg ataccgcgaa agagcagatt tataaccgct tcacactgac gccggaaggg
     8521 gatgaaccgc ttcccggtgc cgttcacttc ccgaataacc ctaatatttt tgatctgacc
     8581 gaagcgcagc agctgactgc tgaagagcag gtcgaaaaat gggtggatgg caggaaaaaa
     8641 atactgtggg acagcaaaaa gcgacgcaat gaggcgctcg actgcttcgt ttatgcgctg
     8701 gcggcgctgc gcatcagtat ttcccgctgg cagctggatc tcagtgcact gctggcgagc
     8761 ctgcaggaag aggatggtgc agcaaccaac aagaaaacac tggcagatta cgcccgtgcc
     8821 ttatccggag aggatgaatg acgcgacagg aagaacttgc cgctgcccgt gcggcactgc
     8881 atgacctgat gacaggaaaa cgggtggcaa cggtacagaa agacggacgg agagcggagt
     8941 ttacggccac ttccgtgtct gacctgaaaa aatacattgc ggagctggaa gtgcagacct
     9001 gcatgacaca gcgacgcagg ggacctgcag gattttatgt atgaaaacgt ccaccattcc
     9061 cacccttctg gggccggacg gcatgacatc gctgcgtgaa tatgccggtt atcacggcgg
     9121 tggcagcgga tttggtgggc agttgcgggc gtggaaccca ccgagtgaaa gtgtggatgc
     9181 agccctgctg cccaacttta cccgtggcaa tgcccgcgca gacgatctgg tacgcaataa
     9241 cggctatgcc gccaacgcca tccagctgca tcaggatcat atcgtcgggt cttttttccg
     9301 gctcagtcat cgcccaagct ggcgctatct gggcatcggg gaggaagaag cccgtgcctt
     9361 ttcccgcgag gttgaagcgg catggaaaga gtttgccgaa gatgactgtt gctgcattga
     9421 cgttgagcga aaacgcacgt ttaccatgat gattcgggaa ggtgtggcca tgcacgcctt
     9481 taacggtgaa ctgttcgttc aggccacctg ggataccagt ccctcgcgac tgttccggac
     9541 acagttccgg atggtcagcc cgaagcgcat cagcaacccg aacaataccg gcgacagccg
     9601 gaactgccgt gccggtgtgc agattaatga cagcggtgcg gcgctgggat attacgtcag
     9661 cgaggacggc tatcctggct ggatgccgca gaaatggaca tggatacccc gtgagttacc
     9721 cggcgggcgc gcctcgttca ttcacgtttt tgaacccgtg gaggacgggc agacccgcgg
     9781 tgcaaatgtg ttttacagcg tgatggagca gatgaagatg ctcgacacgc tgcagaacac
     9841 gcagctgcag agcgccattg tgaaggcgat gtatgccgcc accattgaga gtgagctgga
     9901 tacgcagtca gcgatggatt ttattctggg cgcgaacagt caggagcagc gggaaaggct
     9961 gaccggctgg attggtgaaa ttgccgcgta ttacgccgca gcaccggtcc ggctgggagg
    10021 cgcaaaagtg ccgcacctga tgccggggga ctcactgaac ctgcagacgg ctcaggacac
    10081 ggataacggc tactccgtgt ttgagcagtc actgttgcgg tatatcgctg ccgggctggg
    10141 tgtctcgtat gagcagcttt cccggaatta cgcccagatg agctactcca tggcacgggc
    10201 cagtgcgaac gagtcgtggg cgtactttat ggggcggcga aaattcgtcg catcccgtca
    10261 ggcgagccag atgtttctgt gctggctgga agaggccatc gctcgccgcg tggtgacgtt
    10321 accttcaaaa gcgcgcttca gttttcagga agcccgaagt gcctggggga actgcgactg
    10381 gataggctcc ggtcgtatgg ccatcgatgg tctgaaagaa gtacaggaag cggtgatgct
    10441 gatagaagcc ggactgagta cctacgagaa agagtgcgca aaacgcggtg acgactatca
    10501 ggaaattttt gcccagcagg tccgtgaaac gatggagcgc cgtgcagccg gtcttaaacc
    10561 gcccacctgg gcggctgcgg catttgaatc cgggctgcga caatcaacag aggaggagaa
    10621 gagtgacagc agagctgcgt aatctcccgc atattgccag tatggccttt aatgagccgc
    10681 tgatgcttga acccgcctat gcgcgggttt tcttttgtgc gcttgcaggc cagcttggga
    10741 tcagccgcct gacggatgcg gtatccggcg acagcctgac tgccggagag gcacccgcgg
    10801 cgctggcgtt atccgttgat gatgacggac cacgacaggc ccgcagttat caggtcatga
    10861 acggcatcgc cgtgctgccg gtgtccggca cgctggtcag tcggacgcga gcgctgcagc
    10921 cgtattcggg gatgaccggt tacaacggca ttatcgcccg tctgcaacag gctgccagcg
    10981 acccgatggt ggacggcatt ctgctcgata tggatacgcc aggcgggatg gtggcagggg
    11041 catttgactg cgctgacatc atcgcccgtg tgcgtgacat aaagccggta tgggcgctgg
    11101 ccaacgacat gaactgcagt gcaggtcagt tgcttgccag cgccgcctcc cgacgtctgg
    11161 tcacgcagac cgcccggaca ggctccatcg gcgtcatgat ggctcacagt aattacggtg
    11221 ctgcgctgga gaaacagggc gtggaaatca cgctgattta cagcggcagc cataaggtgg
    11281 atggcaaccc ctacagccat ctaccggatg atgtccggga aacactgcag tcccggatgg
    11341 atgcaacccg ccggatgttt gcacagaagg tgtcggcata taccggcctg tccgtgcagg
    11401 ctgtgctgga taccgaggct gcagtgtaca gcggtcagga ggccattgat gccggactgg
    11461 ctgatgaact tgttaacagt accgatgcga tcaccgtcat gcgtgatgca ctggatgcac
    11521 gtaaatcccg tctctcagga gggcgaatga ccaaagagac tcaatcaaca actgtttcag
    11581 ccactgcttc gcaggctgac gttactggcg tagtgcaagc gacggatggc gagaacgcca
    11641 gcgctgcgca gccggacgtg aacgcgcaga tcaccgcagc ggttgcggca gaaaacagtc
    11701 gcattatggg gatcctcaac tgtgaggagg ctcacggacg cgaagaacag gcacgcgtgc
    11761 tagccgaaac ccccggtatg accgtggaaa cggcccgccg cattctggca gctgcaccac
    11821 agagtgcaca ggcgcgcagt gacactgcgc tggatcgtct gatgcagggg gcaccggcac
    11881 cgctggctgc aggtaacccg gcatctgatg ccgttaacga tttgctgaac acaccagtgt
    11941 aagggatgtt tatgacgagc aaagaaacct ttacccatta ccagccgctg ggcaacagtg
    12001 acccggcaca tacggcaacc gcgcccggcg gattgagtgc gaaagcgcct gcaatgaccc
    12061 cgctgatgct ggatacctcc acccgtaagc tggttgcgtg ggatggcacc accgacggtg
    12121 ctgccgttgg cattctggcg gttgatgctg accagaccag caccacgctg acgttctaca
    12181 agtccggcac gttccgttat gaggatgtgc tctggccgga ggctgccagc gacgagacga
    12241 aaaaacggac cgcgtttgcc ggaacggcaa tcagcatcgt ttaactttac ccttcatcac
    12301 taaaggccgc ctgtgcggct ttttttacgg gattttttta tgtcgatgta cacaaccgcc
    12361 cagctgctgg cggcaaatga gcagaaattt aagtttgatc cgctgtttct gcgtctcttt
    12421 ttccgtgaga gctatccctt cactacggag aaagtctatc tctcacaaat tccgggactg
    12481 gtaaacatgg cgctgtacgt ttcgccgatt gtttccggtg aggttatccg ttcccgtggc
    12541 ggctccacct ctgaatttac gccgggatat gtcaaaccca agcatgaagt gaatccgcag
    12601 atgaccctgc gtcgcctgcc ggatgaagat ccgcagaatc tggcggaccc ggcttaccgc
    12661 cgccgtcgca tcatcctgca gaacatgcgt gacgaagagc tggccattgc tcaggtcgaa
    12721 gagatgcagg cagtttctgc cgtgctcaag ggcaaataca ccatgaccgg tgaagccttc
    12781 gatccggttg aggtggatat gggccgcagt gcggcgaaca acatcacgca gtccggcggc
    12841 acggagtgga gcaagcgtga caagtccacg tatgacccga ccgacgatat cgaagcctac
    12901 gcgctgaacg ccagcggcgt ggtgaatatc atcgtgtttg atccgaaagg ctgggcgctg
    12961 ttccgttcct tcaaagccgt caaggagaag ctggataccc gtcgcggctc tcattccgag
    13021 ctggagacag cggtgaaaga cctgggtaaa gcggtgtcct ataaggggat gtatggcgat
    13081 gtggccatcg tcgtgtattc cggacagtac gtggaaaacg gcgtcaaaaa gaacttcctg
    13141 ccggacaaca cgatggtact ggggaacact caggcacgcg gtctgcgcac ctatggctgc
    13201 attcaggatg cggacgcaca gcgcgaaggc attaacgcct ctgcccgtta cccgaaaaac
    13261 tgggtgacca ccggcgatcc ggcgcgtgag ttcaccatga ttcagtcagc accgctgatg
    13321 ctgctggctg accctgatga gttcgtgtcc gtacaactgg cgtaatcgtg gcccttcggg
    13381 gccattttct ctctgtggag gagtccatga cgaaagatga actgattgtc cgtctccggt
    13441 cgctgggtga gcaactgaac cgtgatgtca gcctgacggg gacgaaagaa gaactggcgc
    13501 tccgtgtggc agagctggaa gaggagcttg atgacacgga tgacgctgcc ggtcaggata
    13561 cccctctcag accggaaaat gcgctgaccg gacatgaaaa tgaggtggta tcagcacaga
    13621 cggataccgt gactgatacg gctgctctgg tcacggttgt ggcactggtg acgctgcata
    13681 ccgatgcact tcacgccacg cgggatgaac ctgtggcatt tgtgctgtcg ggaacggcgt
    13741 ttcgtgtctc tgccggtgtg gcagccgaaa tgacagaacg tggcctggcc agaatgcaat
    13801 aacgggaggc gctgtggctg atttcgataa cctgttcgat gctgccattg cccgcgccga
    13861 tgaaacgata cgcgggtaca tgggaacgtc agccaccatg acatccggtg agcagtccgg
    13921 cgcagtaata cgtggtgttt ttgatgaccc tgaaaatatc agctatgccg gacagggcgt
    13981 acgcgttgaa ggctccagcc cgtccctgtt tgtccggact gatgatgtgc ggcagctgcg
    14041 gcgcggcgac acgctgacca tcggtgagga aaacttctgg atagaccgga tttcgccgga
    14101 tgatggcgga agctgtcatc tctggcttgg gcggggcgta ccgcctgccg ttaaccgtcg
    14161 ccgctgaaag ggggatgtat ggccataaaa ggtcttgagc aggccgttga aaacctcagc
    14221 cgtatcagca gaacggcggt gcccggtgcc gccgcaatgg ccattaaccg cgttgcttca
    14281 tccgcgatat cgcagtcggc gtcacaggtt gcccgtgaga caaaggtacg ccggaaactg
    14341 gtaaaggaaa gggccaggct gaaaagggcc acggtcaaaa atccgcaggc cagaatcaag
    14401 gttaaccggg gggatttgcc cgtaataaag ctgggtaacg cgcggattgt cctgtcccga
    14461 cgcaggcgtc gtaaaaaggg gcagcgttca gccctgaaag gtggcggcag cgtgcttgtg
    14521 gtgggaaacc gtcgtattcc ctgcgcgttt attcagcaac tgaaaaatgg ccgctggcat
    14581 gtcatgcagc gtgtggccgg gaaaaaccgt taccccattg atgtggtgaa aatcccgatg
    14641 gcggtgccgc tgaccacggc gtttaaacag aatattgaga ggatacggcg tgagcgtctt
    14701 ccgaaagagc tgggctatgc gctgcatcat caactgagga tggtaataaa acgatgaaac
    14761 atactgaact ccgtgcagcc gtactggatg cactggagaa gcatgacacc ggggcgacgt
    14821 tttttgatgg tcgccccgct gtttttgatg aggaagattt tccggcagtt gccgtttatc
    14881 tcaccggcgc tgaatacacg ggcgaagagc tggacagcga tacctggcag gcggagctgc
    14941 atatcgaagt tttcctgcct gctcaggtgc cggattcaga gctggattcg tggatggagt
    15001 cccggattta tccggtgatg agcgatatcc cggcactgtc agatttgatc accagtatgg
    15061 tggccagtgg ctatgactac cggcgcgacg atgatgcggg cctgtggagt tctgccgatc
    15121 tgacttatgt cattacctat gaaatgtgag gacgatatgc ctgtaccaaa tccagcaata
    15181 ccggtgaaag gtgccggaac caccctgtgg gtttataacg ggagcggcga cccttatgcg
    15241 aacccgcttt cagacgttga ctggtcgcgt ctggcaaaag ttaaagacct gacgcccggc
    15301 gaactgaccg ctgagtccta tgacgacagt tatctcgatg atgaagatgc ggactggacc
    15361 gcgaccgggc aggggcagaa atccgccgga gataccagct tcacgctggc gtggatgccc
    15421 ggagagcagg ggcagcaggc gctgctggcg tggtttaatg aaggtgatac ccgtgcctat
    15481 aaaatccgct tcccgaacgg cacggtcgat gtgttccgtg gctgggtcag cagtatcggt
    15541 aaggcggtga cggcgaagga agtgatcacc cgtacggtga aggtcaccaa tgtgggccgt
    15601 ccgtcaatgg cagaagatcg cagtacggtg acggcggcaa ccggtatgac cgtgacgcct
    15661 gccagctcct cggtggtgaa agggcagggc accacgctga ccgtggcatt ccagccggag
    15721 ggcgcaactg acaagagttt ccgtgcggtg tcagcggata aaacaaaagc cacagtgtcg
    15781 gtcagtggta tgaccatcac cgtgaaaggc gttgctgcag gtaaggtcaa cattccggtt
    15841 gtatccggta atggtgaact tgctgcggtt gcagaaatca ctgtcaccga cagttaatcc
    15901 ggagagtcag cgatgttcct gaaaaccgaa tcatttgaac ataacggcgt gaccgtcacg
    15961 ctttctgaac tgtcagccct gcagcgtatt gagcatctcg ccctgatgaa acggcaggca
    16021 gaacaggcgg agtcagacag caaccggaag tttactgtgg aagacgccat cagaaccggt
    16081 gcttttgtgg tagcgatgtc cctgtggcat aaccatccgc agaagacaaa gctgccttcc
    16141 atgaatgaag ccgttaaaca gattgagcag gaagtgctta ccacctggcc tacagaggca
    16201 atttctcatg ctgaaaacgt ggtgtaccgg ctgtccggta tgtatgagtt tgtggtgaat
    16261 gatgcccctg aacaggcaga ggacgccggg tctgcagagc ctgtttctgc gggaaagtgt
    16321 tcgacggtga gctgagtttt gccctgaaac tggcgcgtga gatggggcga cctgactggc
    16381 gcgccatgct tgccgggatg tcatccacgg agtatgccga ctggcaccgc ttttacagta
    16441 cccattattt tcatgatgtt ctgctggata tgcacttttc cgggctgacg tacaccgtgc
    16501 tcagcctgtt tttcagcgat ccggatatgc atccgctgga tttcagtctg ctgaaccggc
    16561 gcgaggctga cgaagagcct gaagatgatg tgctgatgca gaaagcggca gggcttgccg
    16621 gaggtgtccg ctttggcccg gacgggaatg aagttatccc tacttccccg gatgtggcgg
    16681 acatgacgga ggatgacgta atgctgatga cagtatcaga aggaatcgca ggaggagtcc
    16741 ggtatggctg aaccggtagg cgatctggtc gttgatttga gtctggatgc ggccagattt
    16801 gacgagcaga tggccagagt caggcgtcat ttttccggta cggaaagtga tgcgaaaaaa
    16861 acagcggcag tcgttgaaca gtcgctgagc cgacaggcgc tggctgcaca gaaagcgggg
    16921 atttccgtcg ggcagtataa agccgccatg cgtatgctgc ctgcacagtt caccgacgtg
    16981 gccacgcagc ttgtaggcgg gcaaagtccg tggctgatcc tgctgcaaca gggtggtcag
    17041 gttaaggact ccttcggcgg gatgatcccc atgttcaggg ggcttgccgg tgcgatcacc
    17101 ctgccgatgg tgggggccac ctcgctggcg gtggcgaccg gtgcgctggc gtatgcctgg
    17161 tatcagggca actcaacctt gtccgatttc aacaaaacgc tggtcctttc cggcaatcag
    17221 gcgggactga cggcagatcg tatgctggtc ctgtccagag ccgggcaggc ggcagggctg
    17281 acgtttaacc agaccagcga gtcactcagc gcactggtta aggcgggggt aagcggtgag
    17341 gctcagattg cgtccatcag ccagagtgtg gcgcgtttct cctctgcatc cggcgtggag
    17401 gtggacaagg tcgctgaagc cttcgggaag ctgaccacag acccgacgtc ggggctgacg
    17461 gcgatggcac gccagttcca taacgtgacg gcggagcaga ttgcgtatgt tgctcagttg
    17521 cagcgttccg gcgatgaagc cggggcattg caggcggcga acgaggccgc aacgaaaggg
    17581 tttgatgacc agacccgccg cctgaaagag aacatgggca cgctggagac ctgggcagac
    17641 aggacagcgc gggcattcaa atccatgtgg gatgcggtgc tggatattgg tcgtcctgat
    17701 accgcgcagg agatgctgat taaggcagag gctgcgttta agaaagcaga cgacatctgg
    17761 aatctgcgca aggatgatta ttttgttaac gatgaaacgc gggcgcgtta ctgggatgat
    17821 cgtgaaaagg cccgtcttgc gcttgaagcc gcccgaaaga agactgagca gcagagtcaa
    17881 caggacaaaa atgcgcagca gcagagcgat accgaagcgt cacggctgaa atacaccgaa
    17941 gaggcgcaga aggcttacga acgcctgcag acgccgctgg agaaatatac cgcccgtcag
    18001 gaagaactga ataaggcact gaaagacggg aaaatcctgc aggcagatta caacacgctg
    18061 atggcggcgg cgaaaaaggg ctatgaagcg acgctgaaaa aaccgaaaca gtccggcgtg
    18121 aaggtgtctg cgggcgatcg tcaggaagac agtgctcatg ctgccctgct gacgcttcag
    18181 gctgaactcc ggacgctgga gaagcatgcc ggagcaaatg agaaaatcag ccagcagcgc
    18241 cgggatttgt ggaaggcgga gagtcagttc gcggtactgg aggaggcggc gcaacgtcgc
    18301 cagctgtctg cacaggagaa atccctgctg gcgcataaag atgagacgct ggagtataaa
    18361 cgccagttgg ctgcacttgg cgacaaggtt acgtatcagg agcgcctgaa cgcgctggcg
    18421 cagcaggcgg ataaattcgc acagcagcaa cgggcaaaac gggccgctat tgatgcgaaa
    18481 agccgggggc tgactgaccg gcaggcagaa cgggaagcca cggaacagcg cctgaaggaa
    18541 cagtatggcg ataatcctct ggcgctgaat aacgtcatgt cagagcagaa aaagacctgg
    18601 gcggctgaag acctgcttcg cgggagctgg atggcaggcc tcaggtccgg ctggagcgag
    18661 tggaaagaga gtgccacgga cagtatgtcg caggttaaaa gtgctgccac gcagaccttt
    18721 gatggtattg cgcagaatat ggcggcgatg ctgaccggca gtgaacagaa ctggcgcagc
    18781 ttcacccgct ccgtgctgtc catgatgaca gaaattctgc ttaagcaggc aatggtgggg
    18841 attgtcggga gtatcggcag cgccattggc ggggctgttg gtggcggcgc atccgcgtca
    18901 ggcggtacag ccattcaggc agctgcggcg aaattccatt ttgcgaccgg agggtttacg
    18961 ggaaccggcg gcaaatatga gccagcgggg attgttcacc gtggtgaatt tgtcttcact
    19021 aaggaggcaa ccagccggat tggcgtgggg aatctctacc ggctgatgcg cggctatgcc
    19081 accggcggtt atgtcggtac accgggcagc atggcggaca gccgctcgca ggcgtccggg
    19141 aagtttgagc agaataacca tgtggtgatt aacaacgacg gcacgaacgg tcagataggg
    19201 ccacaggcgc tgaaggcggt ttatgacgta gcccataagg cggcaatgga tgttgtgacc
    19261 gggcagatgc gcgatggtgg tctgttctcc ggaggtggac gatgaaaacc ttccgctgga
    19321 aagtgaaacc cggtatggat gtggcttcgg ccccttccgt caggaaagtg cgctttggtg
    19381 atggctattc ccagcgagcg cctgccgggc tgaatgccga cctgaaaacg tacagcgtga
    19441 cgctttctgt tccccgttgg gaggccacgg cgctggagtc gtttctggct gagcacgggg
    19501 gctggaaatc ctttctgtgg acgccgcctt atgagtggcg gcagataaag gtgacctgcg
    19561 caaaatggtc gtcgcgggtc agtatgctgc gtgttgagtt cagcgcagag tttgaacagg
    19621 tggtgaactg atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca
    19681 gtcggccagc gtggtgctct gggaaatcga tctgacagag gtcggtggag aacgttattt
    19741 tttctgtaat gagcagaacg aaaaaggtga gccggttacc tggcaggggc ggcagtatca
    19801 ggcatacccc attcagggga cgggatttga actgaacggc aagggcagtg ctgcccgtcc
    19861 gacactgacg gtttctaacc tgcacggcat ggtcaccggg atggcggaag acctgcagag
    19921 tctggtcggc ggaacggtgg tcaggcgtaa ggtttacgcc cgttttctgg atgcggtgaa
    19981 cttcgtcaac ggaaacagtg acgccgatcc ggagcaggag gtgatcagcc gctggcgcat
    20041 cgagcagtgc agcgaactga gcgcggtgag tgcctccttt gtactgtcca cgccgacgga
    20101 aacggatggc gctgtttttc cgggacgtat catgctggcc aacacctgca cctggaccta
    20161 tcgcggtgac gagtgcggtt atagcggtcc ggctgtcgcg gatgaatatg accagccaac
    20221 gtccgatatc acgaaggata aatgcagcaa atgcctgagc ggttgtaagt tccgcaataa
    20281 cgtcggcaac tttggcggct tcctttccat taacaaactt tcgcagtaaa tcccatgaca
//
LOCUS       DADSZD010000080        19425 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.080, whole genome shotgun sequence.
ACCESSION   DADSZD010000080 DADSZD010000000
VERSION     DADSZD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.080"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            183..2573
                     /locus_tag="J1Z28_002584"
     CDS             183..2573
                     /locus_tag="J1Z28_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose/quinate/shikimate family membrane-bound
                     PQQ-dependent dehydrogenase"
                     /protein_id="HBA7717411.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(2769..4319)
                     /gene="cueO"
                     /locus_tag="J1Z28_002585"
     CDS             complement(2769..4319)
                     /gene="cueO"
                     /locus_tag="J1Z28_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBA7717412.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHQLEHEDT
                     GMMLGFTV"
     gene            4485..4832
                     /locus_tag="J1Z28_002586"
     CDS             4485..4832
                     /locus_tag="J1Z28_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBA7717413.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            4938..5804
                     /gene="speE"
                     /locus_tag="J1Z28_002587"
     CDS             4938..5804
                     /gene="speE"
                     /locus_tag="J1Z28_002587"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBA7717414.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALALQPS"
     gene            5820..6614
                     /gene="speD"
                     /locus_tag="J1Z28_002588"
     CDS             5820..6614
                     /gene="speD"
                     /locus_tag="J1Z28_002588"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBA7717415.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(6652..7014)
                     /locus_tag="J1Z28_002589"
     CDS             complement(6652..7014)
                     /locus_tag="J1Z28_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBA7717416.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(7189..9786)
                     /gene="acnB"
                     /locus_tag="J1Z28_002590"
     CDS             complement(7189..9786)
                     /gene="acnB"
                     /locus_tag="J1Z28_002590"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBA7717417.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            10141..11898
                     /locus_tag="J1Z28_002591"
     CDS             10141..11898
                     /locus_tag="J1Z28_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBA7717418.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPIALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSGQSWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVITIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHYDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGMPYHDQDMTKRFHQTDVKGGMSA
                     TPLPAPTRDSQRQAAASQFQQRTHSAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQQREAARERIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(12140..13564)
                     /gene="lpdA"
                     /locus_tag="J1Z28_002592"
     CDS             complement(12140..13564)
                     /gene="lpdA"
                     /locus_tag="J1Z28_002592"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBA7717419.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(13772..15664)
                     /gene="aceF"
                     /locus_tag="J1Z28_002593"
     CDS             complement(13772..15664)
                     /gene="aceF"
                     /locus_tag="J1Z28_002593"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBA7717420.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGMKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(15679..18342)
                     /gene="aceE"
                     /locus_tag="J1Z28_002594"
     CDS             complement(15679..18342)
                     /gene="aceE"
                     /locus_tag="J1Z28_002594"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBA7717421.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETLEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(18503..19267)
                     /gene="pdhR"
                     /locus_tag="J1Z28_002595"
     CDS             complement(18503..19267)
                     /gene="pdhR"
                     /locus_tag="J1Z28_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBA7717422.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         4491 a         5341 c         4875 g         4718 t
ORIGIN      
        1 acaaatcact tcgcacgggc aggtttcagc accgcaacaa agcgcaagac acgcagtata
       61 ccagtaaaac tattcattcg cggaatctgt taataaagct cgaattgtga tgacgatcac
      121 acatgttaaa acctataatt aattgctact aaaatattaa tgaattgaaa tggtgtctct
      181 ttatggcaat taacaatacc ggctcgcggc gattactcgt cacgctaaca gccctttttg
      241 cagcgctttg cgggctgtat ctactcattg gcggaggctg gctggtcgcg attggcggct
      301 cctggtacta ccctatcgct ggccttgtga tgctcggcgt cgcctggatg ctgtggcgca
      361 gtaaacgcgc cgcgctttgg ctgtacgcag ccctgctgct cggcactatg atttggggcg
      421 tctgggaagt tggtttcgac ttctgggcgc tgactccgcg cagcgacatt ctggtcttct
      481 tcggcatctg gctgatcctg ccgtttgtct ggcgtcgcct ggtcattcct gccagcggcg
      541 cagttgccgc actggtggtc gcactgctga ttagcggtgg tatcctgacc tgggccggat
      601 ttaacgatcc gcaggaaatc agcggcacct taagcgccga taccacgcca gcagaagcca
      661 tctcccccgt tgccgatcag gactggcctg cctatggtcg caatcaggaa ggtcaacgct
      721 tttcaccact gaagcaaatt aacgctgata acgtccacaa gctgaaagaa gcctgggtgt
      781 tccgtactgg cgatgtgaag cagccgaacg atccgggcga aatcaccaat gaagtgacgc
      841 caattaaagt gggcgacacc ctttacctgt gtaccgcgca ccaacgtctg tttgcgctgg
      901 atgccgccag cggtaaagag aaatggcatt acgatcctga gctgaaaacc aacgagtcct
      961 tccagcatgt aacctgccgt ggcgtctctt atcatgaagc caaagcagaa actgcttcgc
     1021 cggaagtgat ggcggattgc ccgcgtcgta tcattcttcc ggtcaatgat ggtcgtctga
     1081 ttgcaattaa cgctgaaaac ggcaagctgt gcgaaacctt cgccaataaa ggcgtgctca
     1141 atctgcaaag caatatgccg gacaccaaac cgggtctgta tgagccgact tcgccgccga
     1201 ttatcaccga taaaaccatc gtgatggccg gttcagtcac cgacaacttc tcaacccgcg
     1261 aaacgtctgg cgtgatccgt ggttttgatg ttaacaccgg ggagctgctg tgggcttttg
     1321 atccgggcgc gaaagatccg aatgcgatcc cgtctgacga acacaccttt acctttaact
     1381 caccaaactc gtgggcacca gcggcctatg acgcgaagct ggatctggtc tatctgccga
     1441 tgggcgtgac cacgccagat atctggggcg gtaatcgcac accggaacag gaacgttatg
     1501 ccagctcgat tctggcgctg aatgccacta ccgggaaact ggcgtggagc taccagaccg
     1561 ttcaccacga cctgtgggat atggatcttc cggcacagcc gacgctggcg gacatcaccg
     1621 ttaatggtca gaaagtgccg gtcatttatg ctccggcgaa aaccggcaac atttttgtgc
     1681 tcgatcgtcg caatggtgaa ctggtggttc ctgccccgga aaaaccggtt ccccaaggtg
     1741 ctgctaaagg cgactacgtg acccctactc aacccttctc ggaactgagc ttccgtccga
     1801 cgaaagattt gagcggtgcg gatatgtggg gagccaccat gtttgaccag ttggtgtgcc
     1861 gcgtgatgtt ccaccagatg cgctatgaag gcattttcac gccgccatct gaacagggta
     1921 cgctggtctt cccgggtaac ctggggatgt tcgaatgggg cgggatttcc gttgatccga
     1981 atcgtgaagt ggcgattgcc aacccaatgg cactgccgtt tgtttcgaaa ctgatcccac
     2041 gcggtcctgg taacccgatg gaacagccga aagatgccaa aggcacgggt acggaatccg
     2101 gcatccagcc gcagtacggt gtaccgtatg gcgtcacgct caacccgttc ctctcaccgt
     2161 ttggtctgcc atgtaaacag ccagcatggg gctatatctc ggcattggat ctgaaaacca
     2221 atgaagtggt gtggaagaaa cgtattggta cgccgcagga cagtatgcca ttcccgatgc
     2281 ctgtaccggt gccgttcaat atgggtatgc cgatgcttgg cgggccaatc tccacggcgg
     2341 gtaacgtgct gtttatcgcc gctacggcag ataactacct gcgcgcttac aacatgagca
     2401 acggtgaaaa actgtggcag ggtcgtttac cagcgggtgg tcaggctacg ccaatgacct
     2461 atgaagtgaa tggcaagcag tatgtggtga tctccgcagg cggtcacggt tcatttggta
     2521 cgaagatggg cgactatatt gtggcttatg cgctgccgga tgatgtgaag taaggcttgc
     2581 tcagattgct gacaacgtgc gatttgttca tgccggatgc ggcgtgaacg ccttatccgg
     2641 cctacaaaac atttgcaaat tcaatacatt gcgcgtaggc ctgataagcg tagcgcatca
     2701 ggcagttttg cgtttgtcat cagtttcatg cccggagaga tccgggcata tttccgaata
     2761 cggtcttttt ataccgtaaa ccctaacatc atccccgtat cttcatgctc cagctgatgg
     2821 cagtgcgcca tataagcatg ttctttcggt gcatcgtgat taaacttcac cagcacttcg
     2881 ctgacattac cttctacctt aacggtatct ttccagcctg cgcgatgcgt cgctggcggt
     2941 ttgccatttt ctgacaagat acggaactgc gtaccgtgga tatggaacgg atgcagcatc
     3001 atgtcgccca cgccagagat aacccaacgt tcgtactgcc ctttcgccgc cgcaaacatc
     3061 ggcttattca tatcaaacgc ctgaccgttg attttgttgg catggtggaa atcgaacttc
     3121 ccgctgtggt tcatatgatt catattgccg tgccccatat ggcccatcat ctggctgtga
     3181 tccatcccgg ccatcgcctg atcgccatat ttctccatca gcatctgcat ccccatcata
     3241 tcgagcattg ggtccataga aagttgcagc ttgcgtaccg tcagcccttc cagcgaaggt
     3301 aacgcaggca ggctacttaa tgtgtctggc aaagcaccag aggcactaat ggcaatcggc
     3361 tgaatccgca ttaccggatg cggcttgtca aacggcgcaa tcgccatccc catctggctg
     3421 accggcagcg tcaccaggtc aaagggtttg ttgtcgttaa cctccaccag cacttcaaaa
     3481 cgctcgccca tcagcactgg cagctcgttc accttcaccg gttcaggtag cagaccgccg
     3541 tcgctggcaa tcacataaag cgggcgattg tcgctggtgg cgaaattgag cgagcgggca
     3601 ttacagccat tgagcaaacg caggcgcagc cagccacgcg gggcagcgtg ttgcgggtag
     3661 attgcaccgt tggtcagcaa cgtatcgcca aaccaaccca cagcggcggt catcacatcc
     3721 agttgataat caatctgccc gtcagcgtta aattttttat cctgaacaat caccgggaca
     3781 tcatcaatac cccactgttt tggcagcatt aatttcagga tctcgtcatc ttcaatcacc
     3841 accaaaccag ccagccccat cgccacctga cgtccggtct tgccatgttg atgtggatgg
     3901 aaccagcagg tagcggcagg ttgatcaacg ttcaacgtca ccgagcgctt gccacctggc
     3961 ggaataattc cctgcgggcc gccgtccact tcacccggta cttccagccc gtgccagtgc
     4021 aacgtcgtct cttccgtcag ttggttgtag atatcaaccg ttaccgcttt gccacgctgt
     4081 aatttcaccg ccggccccag cagattgccg ttatagcccc aggtagttgc ggttttcccg
     4141 ccaaaggtgg actgacctgc gccaatagtt aactgaatgc gattacgggc atcggtcgtg
     4201 agcaaatcag ggattggtaa cgttgggcgt tccgccgcaa atactgcgcg gctccacagc
     4261 ggcaaggctg aagccacacc cagcgcgacg gaatatttca agaaatcacg acgttgcata
     4321 gttatttcct tattcttaag caggcgaaac aaaatcaaac gttgagcata gtccttcccc
     4381 ttacgggaag gtcaagtcgc agacacaatt taatcgacaa taatggtcgt cgcctcgctc
     4441 gcagtgtgct aacgtttatc ttctttaagc ccctggtaga agcaatgaag acgtttttca
     4501 gaacagtgtt attcggcagc ctgatggccg tttgcgcaaa cagttacgcg ctcagcgagt
     4561 ctgaagccga agatatggcc gatttaacgg cagtttttgt ttttctgaag aacgattgtg
     4621 gttaccagaa cttacctaac gggcaaattc gtcgcgcgct ggtctttttc gcccagcaaa
     4681 accagtggga tctcagtaat tacgacacct tcgacatgaa agccctcggt gaagacagct
     4741 accgcgatct cagcggcatt ggcattcccg tcgctaaaaa gtgcaaagcc ctggctcgcg
     4801 attccttaag cctgcttgcc tacgtcaaat aatccctgat acctttttgt agaaatattg
     4861 accgtgcatc cgcggtcaat gttagctatt atgttgcgcc atttttttac gggtgttaac
     4921 aaaggaggta tcaacccatg gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt
     4981 ttgggcagta ctttgcggta gataacgttc tgtatcatga aaagaccgat caccaggatc
     5041 tgatcatttt tgagaacgcc gcatttggtc gcgtaatggc gctggatggt gtagtacaaa
     5101 ccaccgagcg tgacgagttt atctatcatg agatgatgac ccatgttccg ctactggccc
     5161 acggtcacgc gaaacatgtg ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag
     5221 taacccgaca taaaaacgtt gagtcaatca cgatggtgga aatcgatgcg ggtgtcgtgt
     5281 cgttctgccg tcagtatctt cccaaccata acgccggtag ttacgacgat ccgcgcttta
     5341 aactggtgat cgacgatggt gtcaatttcg ttaatcaaac caaccagacc tttgatgtca
     5401 ttatctccga ctgcaccgat cctatcggtc ccggcgaaag tctgtttact tctgcatttt
     5461 atgaaggctg caaacgttgc ctgaatcctg gcggtatctt cgtcgcacaa aacggcgtct
     5521 gctttttaca gcaggaagag gccatcgaca gtcatcgcaa actcagccat tacttcagcg
     5581 atgttggctt ttatcaggcg gcgatcccga cctattacgg cggcatcatg acctttgcat
     5641 gggcgacaga taacgacgcc ttacgccatc tctcaaccga aattattcag gcgcgttttc
     5701 tcgcttctgg cctgaaatgc cgttattaca atccggcagt ccatacggca gcttttgcct
     5761 tacctcagta tctgcaagac gcactggctt tacagccgtc ctaaggagaa gataagaaat
     5821 tgaaaaaact gaaactgcat ggctttaata atctgaccaa aagtctgagt ttttgtattt
     5881 acgatatctg ctacgccaaa actgccgaag aacgcgacgg ttatattgct tatatcgatg
     5941 aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg
     6001 ccaatattct taacatcgcc cgccaggatt acgagccaca aggtgccagc gtcactattc
     6061 tggtgagtga agaaccggtt gacccgaaac tcatcgacaa aacagaacac cccggcccac
     6121 tgccagaaac ggtcgttgcc catctcgata aaagtcatat ttgcgtacat acctacccgg
     6181 aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga tattgaagtc tctacctgcg
     6241 gcgtgatttc tccgctgaag gcgctgaatt acctgatcca ccagcttgag tccgatattg
     6301 tgactattga ttatcgcgtg cgcggtttta cccgcgacat taacggtatg aagcacttta
     6361 tcgaccatga gattaattcg attcagaact ttatgtccga agatatgaag gcgctgtatg
     6421 acatggtgga tgtaaacgtc tatcaggaga atatcttcca taccaagatg ttgcttaaag
     6481 agttcgacct taagcactac atgttccaca ccaaaccgga agatttaacc gacagcgagc
     6541 gccaggaaat taccactgcg ctgtggaaag aaatgcgcga gatttattac gggcgcaata
     6601 tgcccgctgt ttaacgtctc aggcggagca cggaggctcc gcctgattta cttacttctg
     6661 ctgcacgaaa ttacggtaag ccgccaccac ctgaagaaaa tcctcaacgc cgcacagcga
     6721 caagctctct tcatcgtagt agttcatccc ctcttccatt tcatcgccag cgaattccag
     6781 ttgattggcg cgaaccatca cctcttcacc atccatccat agcgtgtatt catgccctgc
     6841 ccgttgccag gaacgttcgc tacctttcaa tgcgtgcgcc gcttgttcta cttcatcaag
     6901 caaggccagg ttttctttca cctcttcatt aaaccagtgc ccgaccactt catgccccat
     6961 ggacatgcgc acctttacca ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg
     7021 ttcctctgct cctggcaatg cgccgaaatc gcattgcgcc attgatgtaa ttatcgcagc
     7081 cttaccggag aaaaacagcg gaacggcgat ggtttagaaa taaaaaatgc ccgggaatct
     7141 cattcaccgg gcattgtgtc gtttatgcgc agcgcgtgcg cttacttatt aaaccgcagt
     7201 ctggaaaatc accccgtccg ctttttcggt gtactgagaa agctggtcga agttcagata
     7261 acggtaagta tcaacggctg ttttatctac ctgcgccacg taggtctggt actcttccgg
     7321 cgtcggcagt ttgccaatca gcgccgcaac agcagccagt tccgcagaag ccaggaagac
     7381 attcgcgcca gtacccaaac ggttcgggaa gttacgggta gaggtggaaa ccaccgtcgc
     7441 accgtctgct acacgcgcct ggttacccat acacagggaa cagccaggga tctcgatacg
     7501 agcaccactc ttaccgaaga cgctgtagta gccttcttcg gtcagctgcg cagcgtccat
     7561 acgcgttggc ggtgccaccc acaggcgggt cggcaactga cctttgtgcg catccagcag
     7621 tttaccagcg gcacggaagt gaccgatgtt agtcatgcaa gaaccgataa atacttcgtc
     7681 aatcttctcg ccctgtaccg cagacagcgg acgtgcgtcg tccgggtcat tcggtgcaca
     7741 gaggattggc tctttgatat ccgccagatc gatgtcgatc actgccgcgt attccgcatc
     7801 tgcatcggct tccagcagct caggattcgc cagccatttt tccatgccct gaatacgacg
     7861 ctccagggta cgacgatcgc cgtaaccttc cgcgatcatc cacttcagca ggacgatgtt
     7921 agagttcagg tattcaatga tcggttcttt gttcagcttg atggtacaac cggcagcaga
     7981 acgctcggca gacgcatcgg tcagctcaaa tgcctgctca actttcagat ccggcagacc
     8041 ttcaatttcc agaatgcggc cagagaagat gtttttctta cctttcttct caacggtcag
     8101 cagaccttgt ttgattgcat acagcggaat ggcatgtacc agatcgcgca gggtgatacc
     8161 cggctgcatt ttgcctttga agcgtaccag aacggattcc ggcatatcca gcggcattac
     8221 gcctgttgcg gctgcaaatg ccaccagacc agaacccgcc gggaaagaga taccgatcgg
     8281 gaaacgggta tgggagtcac caccggtacc gacggtatcc ggcagcagca tacggttcag
     8341 ccaggagtgg attacgccgt cacccggacg cagcgacaca ccgccacggt tcataatgaa
     8401 gtccggcagc gtgtggtgcg tgttcacgtc aactggcttc ggatacgccg cggtgtggca
     8461 gaaagactgc atcaccaggt cagccgagaa gcccaggcac gccaggtctt tcagttcatc
     8521 acgggtcatc gggccggtgg tatcctgaga accaacagaa gtcattttcg gttcacagta
     8581 cgcgcccgga cgaatgcctt tcacgccaca ggcacggcct accattttct gcgccagcga
     8641 gaagccgcga tcgctctcag cgacatcttt cgcctgacgg aacacatcgc tgtgcggcag
     8701 gccaagtgct tcacgcgctt tggtggtcag gccacgcccg ataatcagcg gaatacggcc
     8761 gccagcacgc acttcatcaa tcagcacgtc ggttttcagt tcgaaggttg ccagcagttc
     8821 gccggtttcg tggttacgca cttcaccttt gtacgggtaa acgtcaatta cgtcgcccat
     8881 gttcaggtta gagacgtcga cttcgattgg cagtgcaccc gcgtcttcca tcgtgttaaa
     8941 aaagattggt gcaattttac cgccgaggca caaaccaccg ccgcgtttgt tcggcacatg
     9001 tggaatatca tcgcccataa accacagaac ggagttcgtg gcggatttac gcgatgaacc
     9061 cgtacccaca acgtcaccga cgtacgccag cgggaaacct ttctgttgca gagcttcaat
     9121 ctgtttgatc gggccaacaa caccaggctg atctggctca ataccttcac gggcgttttt
     9181 cagcatcgcc agcgcgtgca gtgggatatc cgggcgtgac cacgcatctg gtgccggaga
     9241 gaggtcatcg gtgttggttt cgccagtgac tttgaagacg gtaacggtca gtttttcagc
     9301 cagcgccggg cgattcagga accattcggc atccgcccag gactgcatta cctgcttcgc
     9361 atattcgttg cctgctttgg ctttctcttc tacgtcatag aagttatcga acatcagcag
     9421 cgtgtgagaa agtgctttgg cagcgatcgg tgccagtttg gcatcatcca gcgcatcgat
     9481 cagcggatga atgttgtaac caccctgcat agtgcccagc agttcaatgg ctttttccgg
     9541 agtcagcagc ggggatttag cttcgccttt ggcgacagca gccaggaagc ctgctttgac
     9601 ataggcggct tcatcgacgc cagggggaac acggttggtt aacagatcta acaggaattc
     9661 ttcttcgccc gcgggcgggt ttttcagcag ctctacaagt gcggccattt ggtttgcatc
     9721 caggggtttg ggcgcaatcc cctcagcggc acgctcagct acgtgcttac ggtattcttc
     9781 tagcacgacg gttctcctcg ctctcattgt catagtgcgg caggcgattc tcttcacgct
     9841 cctgtgagac agcagtttgt agggtaaatg cccagtaccg caacgggcag aatagcagga
     9901 ttttgacgat gtgttaatct gtttacaaaa aagcaacata aaaaagtggc tgaatcgtta
     9961 aggatagtct agagaatgtt tccctccgta aaaacttcgt taattcgcat ggtgataatc
    10021 acctttctca acgaatccca agattagtca aaatttaaac tacggcctct ttatactcgg
    10081 attcacagca cctgcgggtg gcagttcgcc gaccattgcg atttccttga gatccgaatt
    10141 atgaaaatga ctttgccgtt taaaccccat gtgctggcgc taatttgcag tgccgggctt
    10201 tgtgccgcct ctaccgggct atatataaaa agccgcacag tggaagcgcc tgtggaagcg
    10261 caatcgacac aacagactgc gcctgacatc tccgcagtta cgcttcctgc aacggtttcc
    10321 gcgcctcctg taacacctgc cgtcgtcaaa tccgcattca gtactgctca aatagatcaa
    10381 tgggttgcac ctatcgcgct ataccccgac tctctgcttt cacaagtgtt aatggcatca
    10441 acctatccgg caaacgttgc tcaagcagtg caatggtcac acgataatcc acttaaacaa
    10501 ggcgatgctg ctattcaggc ggtatctggc cagtcctggg acgccagcgt caaatcactg
    10561 gtggcctttc cacagttgat ggcattgatg ggcgaaaacc cgcaatgggt acaaaacctg
    10621 ggcgatgctt ttctggcgca gccgcaggac gtgatggact cggtacagcg attgcggcaa
    10681 ctggcgcaac aaaccggctc gctgaagtca tcaactgaac aaaagatcat cacgacaacg
    10741 aaaaaagtcg taccggtaaa tcagcccgcc aacgcccctg ccacacaatc aaataccgtt
    10801 tctacgtcca gccccgtcgt cgcagaacct gcaccgaccg taataaccat tgagccagcc
    10861 aatccggatg tggtttatat tccgaactac aacccaaacg tggtttacgg cagttgggcc
    10921 aataccgctt atccgccagt ttatctgccg ccgccagccg gagaaccgtt tgttgacagc
    10981 tttgtgcgcg gattcggcta tagcatgggc gttgctacca catacgcact attcagcagc
    11041 atcgactggg acgacgacga tcatgaccat catcattatg acgatgatga ttatcatcac
    11101 cacgatggcg gtcatcgtga cggtaatgac tggcaacaca acggcgacaa catcaatatc
    11161 gacgtcaaca atttcaaccg tatcaccggt gagcatctta ctgataagaa tatggcatgg
    11221 cggcacaatc caaactaccg taatggtatg ccttatcatg atcaggatat gacaaaacgg
    11281 tttcaccaaa ccgatgttaa aggcggaatg agcgccacgc cgctacctgc tccaacacgc
    11341 gacagccagc gtcaggcggc agcaagtcag tttcagcaac gaacacacag cgcaccagtc
    11401 attacacgag atacccaacg tcaggcagcg gcacagcggt ttaatgaagc tgaaaactat
    11461 gggagctatg acgacttccg cgacttcagc cgtcgccaac cactgaccca gcaacaaaag
    11521 gacgccgctc gtcagcgtta tcagtcggcc tctcctgagc agcgccaggc agtccgcgag
    11581 aaaatgcaga ctaacccgca gatccagcag caaagagagg cagcgcgtga gcgtattcag
    11641 tccgcgtcgc ctgagcagcg ccaggcggtt cgcgagaaaa tgcagactaa cccgcagaat
    11701 caacagcaaa gagatgcagc acgtcagcgt atccagtctg cctcgcctga gcagcgtcag
    11761 gtttttcgag agaaagttca ggagagccgc ccgcagcgtc taaacgacag taaccatact
    11821 gcccgattga ataatgagca acggtcagcg gtacgcgaac ggttgtctga gcgtggagca
    11881 aggagactgg aaaggtaaat tgcaggcgta aaaaagcggc gttgttagcc gcttaagact
    11941 gatgacaaac atcgaatcgc ccgatgcgca agcttatcgg gcctacataa gccatgcaat
    12001 atattgaatt tgcatgattt tgtaggtcgg ataaggcgtt tacgccgcat ccggcatcaa
    12061 ccacgagcac tttatcaaca atctgaagcg gccagaatag ccgcttctta atttgccgga
    12121 tgttccggca aacgaaaaat tacttcttct tcgctttcgg gttcggcagg tcggtaatgc
    12181 taccttcgaa cacttctgcc gccaggccca cagactcgtg cagagtcggg tgcgcatgga
    12241 tggtcagtgc gatgtcttca gcgtcacaac ccatttcgat tgccaggccg atttcaccca
    12301 gcagctcacc gccgttagta ccgacaattg caccaccgat cacacggtga gattctttgt
    12361 cgaaaatcag cttggtcata ccgtctgcgc agtcggaagc gatagcacga ccagaagcag
    12421 cccacgggaa ggtggcggtt tcatagctga tgcctttctc tttcgcttct ttctcagtca
    12481 gacctaccca tgcaacttct ggttcggtat aggcgatgga cgggataact ttcggatcga
    12541 agtagtgttt cttaccggcg ataacttcag cggcaacgtg accttcgtga acacctttgt
    12601 gtgccagcat cggctgaccg acgatatcgc cgatagcaaa gatgtgcggt acgttggtac
    12661 gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac ttccacgcca gctttgcctg
    12721 cgtcgaggtt tttaccgttc ggcacacgac caatcgctac cagcacggcg tcgtaacgct
    12781 gcggttcagc gggtgctttt ttgccttcca tcgtcacata aatgccgtct tctttcgctt
    12841 caacggcggt aactttggtt tccagcatca ggttgaattt cttgctgata cgcttggtaa
    12901 agactttaac gatgtcttta tcagctgccg ggatgacctg gtcgaacatt tcaaccacgt
    12961 caatctgtga acccagcgcg tggtatacgg tgcccatttc cagaccgatg ataccgccac
    13021 ccattaccag caggcgttct ggtacttctt tcagttccag cgcgtcagtg gagtcccaga
    13081 tacgcggatc ttcatgcgga ataaacggca gttgaatcgg gcgagaaccc gctgcaatga
    13141 tcgcgttgtc gaagttgatc acggttttac cgttctcacc ttcaacttcc agggtgttag
    13201 ccccggtaaa tttacccaga ccgttgacca ctttgacttt gcggcctttc gccataccag
    13261 ccagaccacc ggtcagctga ttgatcactt tctctttcca ggtacgaatc ttgtcgatat
    13321 cggttttcgg ttcgccaaag acgataccgt gttcagccag cgctttggct tcttcgataa
    13381 cttttgctac gtgcagcagt gctttagaag ggatacagcc gacgttcagg caaacaccgc
    13441 cgagggtgtt gtagcgttct acgattacgg tttccagacc taaatcagcg caacggaagg
    13501 cagcggagta acctgcgggg cctgccccaa gtaccacgac ctgagttttg atttcagtac
    13561 tcatcatgac ctctatatat ttatctccgg cgggtctgac gtttttttta tgacgcccat
    13621 tatccaccgg gtcgttctat ccgtcggtat tttacaaaat tgttaacaat ttttaaacaa
    13681 caaacggcaa ccgatttgtc tattcgctaa taatctcaat acattcatga gattaccaga
    13741 aaaaagccgg ccgttgggcc ggctctttta tttacatcac cagacggcga atgtcagaca
    13801 gcgtgttgtt aatgatggta atgaaacggg caccatcagc accgtcgatc acgcggtggt
    13861 cgaaggagag agaaatcggc agcatcagac gcggcacgaa ctctttacca ttccacaccg
    13921 gctccatcgc ggacttggaa acgccgagga tagccacttc cggcgcgttc acaatcggcg
    13981 cgaagtgggt agtacccagg ccgccgatgc tggagatggt gaagcaaccg ccctgcattt
    14041 cgcccgctgt cagcttaccg tcacgcgctt tcttagaaat agtcatcagc tcgcgagaca
    14101 gctcgatgat gcctttcttg ttgacgtctt tgaataccgg gacaaccaga ccgttcgggg
    14161 tatccaccgc cacaccgatg ttgatgtatt tcttcagggt cagacgctga ccgtcttccg
    14221 acagcgaact gttgaagcga ggcatctgct caagagctgc agcaacggct ttcatgatga
    14281 agacaaccgg ggtgatcttc acatccagct tacgtttcgc cgcttcttcg ttctgctgtt
    14341 tacggaacgc ttccagctcg gtgatatcgg ttttgtcgaa gtgagtaaca tgcgggatca
    14401 tcacccagtt acggctcagg ttcgcaccag agattttctg gatgcggccc agttccactt
    14461 cttcgatttc accaaacttg ctgaagtcca ccttcggcca cggcagcatg ccagggatac
    14521 caccgccagt tgccgccgga gctgcttctg cacgtttgat agcttctttc acgtaagcct
    14581 gaacgtcttc gcgcaggata cgacctttac ggccggtgcc cttcactttc gccaggttaa
    14641 cgccaaactc gcgtgccaga cggcggatca gcggagtcgc gtgaacgtaa gcgtcatttt
    14701 cagcaaattc agatttgcct tcggcttttg cagccggagc tgctgccggg gcttcagctt
    14761 ttgctgccgg agccggcgct gccgcttcct gcttcgctgg agctgccgca ggcgctgcgc
    14821 cttcaacttc gaagatcata atcagcgaac cagttttcac tttatcgcca acgttgactt
    14881 tcagttcctt cacgacgcct gcaaacggag ccgggacttc catagaagct ttatcgcctt
    14941 ctacggtgat cagtgactgt tcagcggcaa ctttgtcgcc cactttcacc atcacttcgg
    15001 tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac ttctttcatg ccagctgctg
    15061 gtgcaggggc cgctgccgga gccgcttcct gtttagccgc cggagctgcc gcgccagctt
    15121 cacccgcgac ttcgaagacc ataatcagcg agccggtaga cactttgtca cccacgttca
    15181 ctttgatctc tttcacggtg ccagcaaacg gagccggaac ttccatagaa gccttgtcgc
    15241 cttctacggt gatcagcgac tgttcagctt caactttatc gccaactttc accaggattt
    15301 cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt aacgtctttt gccgctgcag
    15361 ccgctggtgc tgctgccgga gcggcttctt tcttctcttc tgcctgagca ggtgcagcgt
    15421 ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc gccggtctgg gttttatcgc
    15481 caacagagac tttgatctct ttaacgatac ccgcctgcgg agatggtact tccatagagg
    15541 ctttgtcgcc ttctacggtg atcagcgact gttcggcttc aactttgtcg cccactttga
    15601 ccaggatctc ggtgatttca acttcatcag ccccgatgtc cggtactttg atttcgatag
    15661 ccattattct tttacctctt acgccagacg cgggttaact ttatctgcat cgatgttgaa
    15721 tttggcgatt gcgtcagcaa ccactttctt atcgatttcg ccacgtttag ccagttcgcc
    15781 cagcgccgca accacgacat aagaagcatc aacttcaaag tggtgacgca ggttctcacg
    15841 gctgtcggaa cgaccgaagc cgtcagtacc cagtacgcgg tagtcgtcag ccggtacgta
    15901 agtacggacc tgctcagcga acagtttcat atagtcggta gatgccactg ccggagcgtc
    15961 gttcatcacc tgagcgatat acggaacgcg cggagtttcc agcgggtgca gcatgttcca
    16021 gcgttcacaa tcctgaccat cacgcgccag ttcggtgaag gaagtcacgc tataaacgtc
    16081 agaacctacg ccgtaatctt tcgccaggat ctctgccgct tcacggacgt gacgcaggat
    16141 agaaccggag cccagcagct gaactttacc tttgctacct tcaagggttt cgagtttgta
    16201 gataccttta cggatacctt cctcagcacc ttccggcatt gccggcatgt ggtagttttc
    16261 gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttt tcaccataca tacgctccag
    16321 accgtcatgc atgatgacag caacttcgta agcgtaagcc gggtcgtaag agatacagtt
    16381 cgggatagtc agcgactgaa tatggctgtg accatcttcg tgctgcagac cttcgccgtt
    16441 cagggtggta cgaccggaag taccgccgat caggaagcca cgcgcttgct ggtcgccagc
    16501 cgcccagcac agatcgccga tacgctggaa accgaacatc gagtaataga tgtagaacgg
    16561 gatcatcggc agattgttgg tgctgtaaga ggtcgccgct gccagccagg aacaacctgc
    16621 gcccagctcg ttgatccctt cctgcagaat ctgacctttc tcgtcttctt tatagtaagc
    16681 aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc gggctgtaaa taccaatctg
    16741 acggaacaga ccttccatac cgaaagtacg cgcttcgtcg gcgatgatcg gtaccagcct
    16801 gtctttgatc gacttgttct tcagcattac gttcagggca cgaacgaaag cgatagtggt
    16861 agagatctct ttgctctgct cttccagcag cgcgccgaag tcttgcaggc tcggcagctc
    16921 aagcttctcg gtgaagttcg gctgacggct tggcagataa ccgtgcagtt tctgacgctg
    16981 agcgtgcaga taggtatgct cttcagaacc ttccgggaag gtgatgtacg gcagtttttc
    17041 gatatctgca tcagacaccg gcacattgaa acggtcgcgg atatgacgca cgccgtccat
    17101 gttcattttc ttaacctggt gcgcgatgtt tttaccttca gccgcgtcgc ccatgccata
    17161 acctttaatg gtatgagcaa ggattaccgt cgctttgcct ttggtttcct gcgctttctt
    17221 gaatgcagcg tagattttct tcggatcgtg accaccacgg ttcagtgccc agatctgctc
    17281 gtcagtccag tctgcaacca gtgctgcggt ttcaggatat ttaccgaaga agtgttcgcg
    17341 aacatacgca ccatctttcg atttgaaggt ctggtaatcg ccgtcaacgg tttcgttcat
    17401 cagctggatc agtttaccgc tggtatcttt acgcagcagt tcgtcccaac ggctacccca
    17461 catcactttg atcacgttcc agccagcacc ttcgaagatg ccttccagtt cgttgatgat
    17521 cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg ttacagttga taacgaagac
    17581 cagattatcc agtttttcac gggtagcgat ggtgatcgca cctttggatt ccggttcgtc
    17641 catttcaccg tcgccgagga acgcgtaaac ggtttgttta gaggtatctt tcaggccacg
    17701 gtgttccaga tatttcagga atttagcctg gtaaatagca ccaatcggac ccagccccat
    17761 agatacggtc gggaactgcc agaattccgg catcagtttc gggtgcggat aggaagagag
    17821 gccattgccg tgaacttcct gacggaagtt atccagctgc tcctgagtca gacgaccttc
    17881 caggaaagca cgagcgtaaa cgcctgggga gatgtggccc tggaagtaaa ccaggtcgcc
    17941 gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag cacacatcat aaatggttgc
    18001 ggaagactgg aaggacgcca tgtggccgcc cagttcgagg tcttttttcg acgcacgcag
    18061 caccgtcatg atggcgttcc agcggatagc tgaacgaata cggcgttcca gttccagatt
    18121 acccggatac tccggttgtt cttcaacggg gatggtgttg atgtagttgc tgataccggt
    18181 gcctgcagct acgttgactc cgcctttgcg ggcttcagca agcagttggt cgatcagata
    18241 ctgagcacgc tcaacacctt cttcacggat gaccgattcg atcgcctgga gccagtcgcg
    18301 agtttcgatc ggatccacgt catttgggaa acgttctgac atgggttatt ccttatctat
    18361 ctaataacgt tgagttttct ggaacctgtc ccattgaact ctcgccggaa agctcaataa
    18421 gacaggttct acgtttagtt gccgcgcttt tatatgcgct tgatttacaa catcttctgg
    18481 ataattttta ccagaaaaat cactaattct ttcgttgctc cagacgacgc agagaacgct
    18541 cacggcggct ctcttcacgg cttctgtcga gcaaaatttc ttcgataaag gccagatggc
    18601 gatgcgatgc ttcgcgcgct tcttccggct taccggccat aatcgcttca aatatgcggg
    18661 tgcggtgact actcaccagc ggcagcatct cgcgacgcga atagagcaat tcgaagttct
    18721 ggcggacatt ctgggccaac atcggctcca tacaccttag cagatgaagc agaaccacat
    18781 tgtgggccgc ttcggtgacg gcaatctgat actggagtac ggcgtttgat tccgcgtcca
    18841 gatcgccaga ctgctgcgcc agctctatgg catggtggag ttcacggatg cgttccttgt
    18901 cttcatcggt actacgcagc gcggcgtaat aagcggcgat accttccagg gcgtgtcgtg
    18961 tttcgagcaa gtcatactgt gattcaggat ggtcggagag cagctccacc agcggatcgc
    19021 tgaagctttg ccataggctg ctctggacaa aagtgccgcc accctggcga cgaagcaaca
    19081 agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga gggacgggag acgtcgaact
    19141 gttttgccag ttcacgttcc ggtgggagtt tttcgcccgg gcggagagtg ccttcgagga
    19201 tcaaaaactc cagttgctgc tcaatcacat cggagagttt tggttggcgg attttgctgt
    19261 aggccatgag ttcctgtctt aagccacttg ccgaagtcaa ttggtcttac caatttcatg
    19321 tctgtgacgc taaagtaaca aagtattcac cttatgtcca tacaggtttt gattgaaatc
    19381 atgaaactgt gcacatttta acaagttgac atatataacg tttca
//
LOCUS       DADSZD010000081        19360 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.081, whole genome shotgun sequence.
ACCESSION   DADSZD010000081 DADSZD010000000
VERSION     DADSZD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.081"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(265..531)
                     /locus_tag="J1Z28_002596"
                     /pseudo
     CDS             complement(265..531)
                     /locus_tag="J1Z28_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            974..1453
                     /locus_tag="J1Z28_002597"
                     /pseudo
     CDS             974..1453
                     /locus_tag="J1Z28_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1452..1994
                     /locus_tag="J1Z28_002598"
                     /pseudo
     CDS             1452..1994
                     /locus_tag="J1Z28_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1994..2986
                     /gene="lsrD"
                     /locus_tag="J1Z28_002599"
     CDS             1994..2986
                     /gene="lsrD"
                     /locus_tag="J1Z28_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBA7717423.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVAVYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVPYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPFP"
     gene            2998..4020
                     /gene="lsrB"
                     /locus_tag="J1Z28_002600"
     CDS             2998..4020
                     /gene="lsrB"
                     /locus_tag="J1Z28_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HBA7717424.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     VPDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            4047..4922
                     /gene="lsrF"
                     /locus_tag="J1Z28_002601"
     CDS             4047..4922
                     /gene="lsrF"
                     /locus_tag="J1Z28_002601"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBA7717425.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVDKGFERIVAGC
                     PVPIVIADGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            4946..5236
                     /gene="lsrG"
                     /locus_tag="J1Z28_002602"
     CDS             4946..5236
                     /gene="lsrG"
                     /locus_tag="J1Z28_002602"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBA7717426.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLEALMTWPRKKRLFNGVMP"
     gene            5293..6051
                     /gene="tam"
                     /locus_tag="J1Z28_002603"
     CDS             5293..6051
                     /gene="tam"
                     /locus_tag="J1Z28_002603"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBA7717427.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(6055..6969)
                     /locus_tag="J1Z28_002604"
     CDS             complement(6055..6969)
                     /locus_tag="J1Z28_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family inner membrane protein"
                     /protein_id="HBA7717428.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(7166..8617)
                     /locus_tag="J1Z28_002605"
     CDS             complement(7166..8617)
                     /locus_tag="J1Z28_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBA7717429.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEALGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(8844..10262)
                     /locus_tag="J1Z28_002606"
     CDS             complement(8844..10262)
                     /locus_tag="J1Z28_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7717430.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(10401..10760)
                     /locus_tag="J1Z28_002607"
     CDS             complement(10401..10760)
                     /locus_tag="J1Z28_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBA7717431.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            complement(10760..11686)
                     /gene="glsB"
                     /locus_tag="J1Z28_002608"
     CDS             complement(10760..11686)
                     /gene="glsB"
                     /locus_tag="J1Z28_002608"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBA7717432.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(11750..13138)
                     /gene="sad"
                     /locus_tag="J1Z28_002609"
     CDS             complement(11750..13138)
                     /gene="sad"
                     /locus_tag="J1Z28_002609"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBA7717433.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WRETNIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDKENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKIAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRN"
     gene            13239..14120
                     /locus_tag="J1Z28_002610"
     CDS             13239..14120
                     /locus_tag="J1Z28_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7717434.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            14198..14281
                     /gene="yneK"
                     /locus_tag="J1Z28_002611"
                     /pseudo
     CDS             14198..14281
                     /gene="yneK"
                     /locus_tag="J1Z28_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            14459..15649
                     /gene="ydeA"
                     /locus_tag="J1Z28_002612"
     CDS             14459..15649
                     /gene="ydeA"
                     /locus_tag="J1Z28_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBA7717435.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSLIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGMLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(15674..16339)
                     /gene="marC"
                     /locus_tag="J1Z28_002613"
     CDS             complement(15674..16339)
                     /gene="marC"
                     /locus_tag="J1Z28_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBA7717436.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            16551..16985
                     /gene="marR"
                     /locus_tag="J1Z28_002614"
     CDS             16551..16985
                     /gene="marR"
                     /locus_tag="J1Z28_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBA7717437.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTP
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            17006..17389
                     /gene="marA"
                     /locus_tag="J1Z28_002615"
     CDS             17006..17389
                     /gene="marA"
                     /locus_tag="J1Z28_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBA7717438.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            17421..17639
                     /gene="marB"
                     /locus_tag="J1Z28_002616"
     CDS             17421..17639
                     /gene="marB"
                     /locus_tag="J1Z28_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBA7717439.1"
                     /translation="MKPLLSAIAAALILFSAQGVAEQTTQQVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(17696..19135)
                     /locus_tag="J1Z28_002617"
     CDS             complement(17696..19135)
                     /locus_tag="J1Z28_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA7717440.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSLEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLNYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(19160..>19360)
                     /locus_tag="J1Z28_002618"
     CDS             complement(19160..>19360)
                     /locus_tag="J1Z28_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717441.1"
                     /translation="VTLNESAYKTTLWHALKVGESILGSRPEIRFYGTYINILDNELS
                     NFKFNENSKDEFMAGIQAEVWW"
BASE COUNT         4957 a         4827 c         4688 g         4888 t
ORIGIN      
        1 gacacctatt cacaacaggt ttattgctgc acttgttgta gagagtattt gtaaaaatat
       61 tttatgtcgc taaataacat gacgatttca ttatgcatgt tatttataaa cagcgactaa
      121 acagaatatt attgcgaaca agggaagtgt caggaaaggt caagaggtac taatagttta
      181 tatggctata aacattagct gtggttaata tttgcctggt gaaactaaca cggaattgct
      241 ctaaacagat aagtgcaggg tttattaaag ctgaccgaca gactttctta acctggtaat
      301 atctctctta ggtgactctc caaacatccg cgaatattcc cgactgaaat gggataagct
      361 ttcgtaaccg acaacatagg ctgcagtggt gacatcgtaa tgttcattca gcatcagtcg
      421 cctggcttca ttgaggcgta gccatttctg ataccggagt ggactcattc ccgcaagctg
      481 tctgaagtgt tgatgaaaag agggggtgtt catttgcacg cggtttgcca gcctgactct
      541 tcaacgcagg gaagatgcgg caaacggcaa acctgcatac cagccagcct atatccggta
      601 tgcacaggcc aatcatgaaa gaggagaggc tttatgttct ggaaccctga agtaataacg
      661 acgaccggaa aatcgcttcg catcactcaa attcaattta acataatata cattatgcgc
      721 accaacataa accaagggag aatccagtcg ttggcggtca tgattgtcct gctcattaac
      781 aacgaccaaa accccatatc ttacttaata ccgtattctc ggcttaaccg gtcgtgcaac
      841 ctcaacacat tggtttcaac tacatcttat ccgctctacg ggataaacgc tgcgatatcc
      901 gcgatcgcgg ataaaaagga ggatttaaaa gtcgtttgaa gtcggcaata ttaagccgca
      961 tgccatcttc gacatgcggc ttatacggtt taccagctat atgaagatta ttgccggtat
     1021 tacccctgct gatagcggta cgctggagat tgggggcaac aactacgtca gattaacgcc
     1081 agttcatgct catcagctgg gcatttatct cgttccccag gaaccgctgc tttttccaag
     1141 cctgtcgata aaagaaaaca tcctgtttgg tctggcaaaa aaacaactct ccatacagaa
     1201 aatgaataac ttgctggcgg cgctgggctg ccagtttgat ctgcatagtc tggcaggatc
     1261 gctggatgtc gccgatcgcc aaatggtgga aatcctccgc gggctgatgc gcgattcgcg
     1321 aattctgatc ctcgatgaac ctaccgcctc gcttactccc gccgaaaccg aacgtttgtt
     1381 tattcgcttg cgggagctgc ttgctactgg cgtgggtatt gtttttatct cgcataagct
     1441 gccggaaatt cggttggttg acgataattc tggtggcatt tatggcctgg ctgctggcaa
     1501 agacggcgtt tggacgcagt ttttatgtca cgggcgataa tttacagggc gctcgtcaac
     1561 tgggcgttcg tacggaagcc attcgcattg tggcattttc gttgaacggc tgcatggcgg
     1621 cactggcggg aattgtgttt gcttcgcaga ttggttttat ccccaaccag accggtaccg
     1681 ggctggagat gaaagcaatt gcagcctgcg tgctgggcgg cattagtttg ctcggcggtt
     1741 ccggcgcgat catcggtgcg gtactcggcg catggttcct gacgcagatc gatagtgtac
     1801 tggtgctgtt gcgcattccg gcatggtgga atgattttat cgcgggcatg gttctgctgg
     1861 cggtgctggt gtttgatgga cgcctgcgtt gtgcgctgga acgtaatctg cggcggcaaa
     1921 aatatgcccg ctttatgatg ccaccgccat ccgttaaacc cgcttcgtca ggtaaaaaac
     1981 gggaggccgc gtaatgcgta ttcgctacgg ttgggaactg gctcttgccg cactgctcgt
     2041 tattgagatt gttgcatttg gtgcaattaa cccgcggatg ttagatctca atatgttgct
     2101 gttcagcacc agtgacttta tctgcattgg cattgtcgcc ctaccgctga cgatggtgat
     2161 tgtcagtggt gggatcgata tttcgtttgg ttcgaccatc ggcctctgcg ccattgcatt
     2221 gggcgtactg tttcaaagtg gtgtgccgat gccgctggcg attcttctga ccttactgct
     2281 cggcgcattg tgcgggttga tcaacgccgg attaattatc tataccaaag ttaacccgct
     2341 ggtgattacg cttggcacgc tgtatctgtt tgccggaagc gccctgctgc tttccgggat
     2401 ggccggagcg acggggtacg aaggtattgg tggattcccg atggcgttta cagatttcgc
     2461 taacctggat gtgctgggac tccccgttcc gctgattatc ttcctgatat gtctcctcgt
     2521 tttctggctc tggctgcata aaacccatgc cggacgtaac gtgtttttaa ttgggcaaag
     2581 cccgcgtgtg gcggtttata gcgcgattcc ggtaaaccgc accttgtgtg cgctctatgc
     2641 catgacgggg ctggcgtctg cggtcgccgc tgtgctgctg gtaccgtatt ttggttcagc
     2701 acgttccgat cttggtgcgt cgtttctgat gcccgccatc accgccgtag tgctcggcgg
     2761 tgccaatatt tatggtggtt ctggttccat tatcggcacc gccattgcgg ttttattagt
     2821 gggatatttg caacaaggtt tgcaaatggc aggagtgcca aatcaggtgt ccagcgccct
     2881 ttccggtgcg ctacttatcg tcgttgtcgt aggtcgttcc gttagcctgc atcgccagca
     2941 aattaaagag tggctggcgc gtcgggccaa taacccattt ccataaagga tatcttcatg
     3001 acacttcatc gctttaagaa aatcgcctta cttagcgcgc ttggcattgc cgcaatctct
     3061 atgaatgtgc aggccgcaga gcgtattgca tttattccca aactggttgg cgtgggattt
     3121 tttaccagcg gtggcaacgg cgcacaacaa gctggtaaag agctgggcgt tgatgtgacc
     3181 tacgacgggc cgacagaacc cagtgtttct ggtcaggtac agttgattaa taactttgtc
     3241 aatcaggggt ataacgccat tatcgtttct gcggtttcgc ctgatggctt gtgtccggca
     3301 ctgaaacgcg ccatgcaacg tggtgtgaga gtgctgacct gggactctga taccaaaccg
     3361 gagtgccgct cttactacat taatcaggga acacccgccc agttgggagg tatgttggtg
     3421 gatatggcgg cgcgtcaggt gaataaagac aaagccaaag tcgcattttt ctactcaagc
     3481 ccaaccgtta cggaccaaaa ccagtgggtg aaagaagcga aagcgaaaat cgccaaagag
     3541 catccaggct gggaaattgt cactacgcag tttggctata acgatgccac taaatcgtta
     3601 caaaccgcag aaggaatatt aaaagcctat agcgatctcg acgccattat cgtccccgat
     3661 gccaatgccc tgcccgctgc cgcacaagcc gcagaaaacc tgaaaaatga caaagttgcg
     3721 attgtcggat tcagtacgcc aaacgtgatg cgtccatatg tggaacgcgg cacggtgaaa
     3781 gaatttggcc tgtgggatgt ggttcagcaa ggcaaaatat cagtttatgt cgcggatgca
     3841 ttattgaaaa aaggatcaat gaaaacgggc gacaagctgg atattcaggg cgtaggtcag
     3901 gtggaagtct cgccaaacag cgttcagggc tatgactacg aagcggatgg taatggcatc
     3961 gtactgttac cggagcgcgt gatattcaac aaagagaata tcggcaaata cgatttctga
     4021 tgtgcattac ttaaacggag tacgttatgg cagatttaga cgatattaaa gatggtaaag
     4081 attttcgcac cgatcaaccg caacaaaata tcccttttat cctgaaaggt tgcggtgcac
     4141 tggattgggg aatgcagtca cgcttatcgc ggatatttaa tccgaaaacg ggtaaaaccg
     4201 tgatgctggc ctttgatcat ggttattttc aggggccgac taccggactt gaacgcattg
     4261 atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt
     4321 tgcgcagcgt agtcccccct gcgaccaata agccggtggt actgcgggcg tcaggtgcga
     4381 actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat gacgccgtgc
     4441 gccttaacag ttgcgcggta gcggcgcagg tttatatcgg cagcgaatat gaacatcagt
     4501 cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg
     4561 ccgtgacagg cgtgggcaaa gatatggtgc gcgatcaacg ttatttctcg ctcgcgactc
     4621 gaatcgccgc tgaaatgggg gcgcagatta tcaaaactta ttatgtcgat aaaggttttg
     4681 aacggattgt tgccggatgt ccggtaccca ttgttattgc tgacggtaaa aaattaccgg
     4741 agcgcgaggc gctggaaatg tgctggcagg ctatcgatca gggcgcttct ggtgtggata
     4801 tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg
     4861 tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt gaaaaacagt
     4921 aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta acgttcatga
     4981 agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga
     5041 agaaggcaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat
     5101 ctacgaagct tataaagatg aagacgcagt ggcgttccat aaaaccacgc cccactacaa
     5161 aacctgtgtc gcaaaactgg aagctttaat gacctggccg cgtaaaaaac gtctgttcaa
     5221 tggggtgatg ccgtgaggcg aatttatcag ttttatctac aattggggta acgcgctgac
     5281 gggagtaaaa aaatgtctga ctggaacccc tctttatatc tgcactttgc cgctgaacga
     5341 tcgcgtccgg cggtggagct gcttgccagg gtgccgctgg agaatatcga atacattgcc
     5401 gatcttggct gtggcccagg taacagcacc gcccttctac accaacgttg gcctgctgcc
     5461 aggataacgg gcatcgactc atctccggcg atgattgctg aagcgcgcag tgctttgcca
     5521 gactgcctgt ttgtggaagc agatatccgc aactggcaac cggaacaggc actcgatctg
     5581 atttttgcta atgcctcgct gcaatggctg cccgaccact acgaattgtt tcctcatttg
     5641 gtttcgttac tcagtccgct gggcgtgctg gcagtacaga tgccagataa ctggctggag
     5701 ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa actacccgga tcgcgggcgt
     5761 gagccgctgg ctggcgttca tgcttactac gatattctga gtgaagccgg atgtgaggtc
     5821 gatatctggc gaaccactta ctatcaccag atgccgtcgc accaggcgat tatcgattgg
     5881 gtgaccgcca ctggattacg tccgtggtta caggatctga ccgagagcga acagcagcat
     5941 tttcttacgc gctaccatca gatgctggaa gagcaatatc ccctccagga gaacggacag
     6001 atattgctgg catttccgcg tctgtttatt gttgcccgcc gtacggagta attatcacgt
     6061 cagctggtaa tggcgatcgg gaagaatttt cgctggaatt tctgcttcat cgttcatctg
     6121 taacaggtca atttcaatag cgttgcagat ggcatccagc ggtaaatcat tgttttcagt
     6181 accgaaggga tcttccagtt cttccgccag acaatccagc gaaataaaag tgtaggaaat
     6241 cagcacagag ataaaaggcg tcatgtaatg caggtccacg accagcgcaa acggcagcat
     6301 gatacaaaac agatacacgg tgcgatgcaa aatcagggtg taggcaaagg gaattggcgt
     6361 ataggcaatg cgctcgcatc ctgccaggac cgctgaaata tcattaagcc gatcgttgag
     6421 gctaataaac aggatatctg aaagttgtcc attgcgtcgc tgaaccgcca accactcccc
     6481 cattattaac aagatacggt tagctggaga gttcgaagcc agtacacgct gaagatcttc
     6541 agtcttgaga taatgagcca gcacttccgc ctgtggctgt ttgcgtaatg tcatgcgtaa
     6601 acagtgggcg aaggcgattt gcagccgggc aaactccctt acacttgccg aatccggcaa
     6661 tgtcgttttt acctcgcgca gtaacgaccg cgaggcaatc atcaactgcc cccaaagttt
     6721 tcgcgcttca acgtaacggg cgtaaccggc attattacga aaaccaagaa aaatggcgat
     6781 ggcgacaccg agaatgctga acggtgcgag ggtgaattta atgcccaaat gcgtgtacca
     6841 gggcagcatg aaaataacag cgatagaaaa gagaaaattg agtagtaagc gcgaggatat
     6901 cttggataat actgaaccgt gccagacaaa aatacggcgc agccagtgtt gttgtggacg
     6961 aacaatcatg gttatcttca ggcgtggaaa agtcgcccta ttaaacgtga ttacgatcac
     7021 attctcaaga cgctacttac aaattaccta caagcttaca acattaacag gtgctgcatg
     7081 tggcacctgt taatgataaa ggcacatagg atgttgtaac taatatagcg aaataagaaa
     7141 cccggtcgaa accgggttca gagtattagc acaacgggcg tacagcttcg cgcatccctt
     7201 tttcgagaat cgcatccagg tcattagcaa cctgctcgac cagaccaggc acttgcgtca
     7261 ggtcctgctc ccagtggtct ttttctgcca acacaatcgc taccagttcc tgggtcccga
     7321 taacacggtc acgatactgg ctccagagtt gctgataacg ttccagccag tgtgcatcgt
     7381 cctgtaccgg atatgtttct ccattacgct caccgcgata gaacgcaatc aatgccgcta
     7441 atgcgaaagt aaggcgcgcc ggaagtgtgc cgtttgcctt ctgccctgcc agcagctgcg
     7501 gcaggatgcg tgtgcggaac ttggtcatac cgttgagcgc gatagacagc agttgatgct
     7561 tgatgtacgg gttacggaaa cgtccggtga ctgcactggc gaaagactcc agctcatcgc
     7621 gtggcaaatc cagtaccgga ataatttctt cgtagatggc tttttcaacg aatgcgcaaa
     7681 tttcagcatc gttcatcgct tcacctacgg tatccagccc tgcctggaac gccaccggca
     7741 ccagcgcggt gtgcgcaccg ttgaggattg ccactttgcg ttctttatac ggtttaatat
     7801 cgtcaacgat cagcacgttg agcggatatt tgtccagacg aagttcagtc gctaaggatt
     7861 tcggcccctg aatcacaaac aggtaaaagt gttcagcggt gtcgaggaaa ccatcgtggt
     7921 agcccagtgc ttcttccagt ttagccactt catcacgcgg ataaccggta acgatacggt
     7981 ccaccagcgt agaacagaag ctgttagcct gatccagcca ttgaataaat gcttctggca
     8041 gtgcccactc ttgcgcatag cgcagcacca gttcacgcaa cgcgtcgcca ttatagtcaa
     8101 tcaactcaca cgggatgatg atccaacctt tatccagcgc accgttgaaa tggctaaaac
     8161 gttcgaacag cagacgggtc agttttgccg gataacttac cgctggcgcg tcatcgaatt
     8221 tatcgcccgc atggtagctg atacccgctt cggtggtgtt agagaaaaca aagcgcattt
     8281 ccggattgtg agccagtttc aggaattcat cgtattcact gtagacgctg atttcacgat
     8341 taaccgagcg aatcaggcgc gcgtcgctga ccgcttcacc cttctcattc aggccacgga
     8401 taatggtggt ataaagacca tcctgcgtgc tcagtgacgg cgggaatgaa gtttcaatcg
     8461 gacgaacaac gaccacgcca gaattcagat cggtgtgctc attcaggaga tcgatttgcc
     8521 agtcaacaaa ggcgcgcagg aagttacctt caccaaactg aatgatacgt tctggatact
     8581 gtgcaccggg aaaatcgcga cgatttagtg ttttcacaat gggttccctt ctgattagtc
     8641 atacaacctg tttgaattgg tacgacaggt tagcaaactt taatacgccg aacccttgtt
     8701 ttgatcaact actgatgatt aatgagcagt tttatgagaa aagtgtggcg cggatcatgg
     8761 tttaatcgag gaaacaggcc ttttcttgga tcataaagtg gtagaacaca ttgcatttaa
     8821 ttcgctctga gtaaataaag atgtcagaca acttcctcac cgtaacgcat agtgctggta
     8881 cggttgcgcc catctttctt cgagcgatac agacacgtat cggcttcgac catcaattta
     8941 ttaaagtcat cggtcagcgt tcggtacgat gcgcaaccac taccgacgcc aatacttacc
     9001 gtgagataaa gcgttttttg ttgccaggtg aacggttgaa gttctacgcc tttacggatt
     9061 ttttccgcca ttagcaggcc atcgacagga ttcaccgacg gcactgcaac agcaaattct
     9121 tcgccgccca ttcgcgccac cagcccctta tcaccgacaa tcttctgaat atgctgggca
     9181 aacacgctta acactttatc gccacattca tgtccatagt tatcgttaat gcttttgaaa
     9241 tagtcgatat caagcagcat gaccgtcaga tgttgtgtct gtttcagcga tggacttttc
     9301 agcgcctcat aaagaccaga ccgtgagtaa acctgagtca gaaaatcaaa gtcggctcgc
     9361 agcgcaactt gcttcattag cgaattgatc gctgccacgc taaaggaaac cataattggg
     9421 catatcgcca tcgtggcaat accgagacgt gcggagaaca tttgcggaat agagaacggc
     9481 gaaccgaccg agatatcaat caccgaattt gccaccagta cgacttccac cgcaccggtg
     9541 acaaaggtca acagacatgt tacctgcggc gtatagcgca ctgcacacca gattaatgca
     9601 gggagcggaa acgccagact ccccgctccg ccaatgacta ccgaggcaat aaccgacaca
     9661 ataagcgcga tagcaggcat catctgctct gctttaaagc gcggcagtac tccaggaatt
     9721 gccaacgtca gcatacaagg cacgatcaac acgcccgttg agaattgctc actaaaccag
     9781 tcggcaagca aaggccagaa atccagacta tcaatactga ccgaaccaat cgccccgaca
     9841 atagcgcata ataatgcggc aagcagacag taattaaata gccgtaaggc acttactggc
     9901 tcatacttat tttttcccag acgcttatcc cgcgcgacca gtaaggcgac ggtaacaata
     9961 aacatcatat tggagaaatt gatagccagt gaaaccagcc cccattcggt ggttatggca
    10021 tcataaacca gcatcgccac ataactgatc gcatagtaat gcagtcgatt aagccagaca
    10081 tagcgggcaa acacccccgc cattacgcca ttcagcggcc agaaaagaga aagttcgtga
    10141 accagccgta actctgcacc aataaaatag aacagcgtgg ttagcacaaa aatagcgatg
    10201 ctattacgta gcagatgacc ttcctggaac aaacggaaag ttgaaatggg ctgtacatgc
    10261 attaattatc tgtccataca attgcgggtg gcaattatct caacgcaata agtaaattct
    10321 tagaaaggat agcatgttcg ctgacaacgg cggcaactaa agatacctaa tgaatgccat
    10381 tttatcgatt tccgtaatta tcatggctga ttcatctgta taaccagcca gtggtagatt
    10441 accgtcacaa tataacgttg ttgctcctca cttaacgctt ccccctgagg aatgttatgc
    10501 cacttagcca gacaaccgcg acaacaggtc gcggtggcgt gttgagcgat aaaaaccgga
    10561 tgcccgcgca tgggggtttg cttaccgtcg ttagccggta atgccggagc aagacgttta
    10621 gcgacaaaat ccgccgcatg ttgttcaatg actggcgcgc ctttctccag acaatactga
    10681 cgctctttca cacccaaacg aaagcgagag cgaaattttg aacgggataa acgcgcgaag
    10741 agtggatcga gagactgcat taataaactg aacgccctaa ttgtttcgtc aattgttcaa
    10801 ggacggcaat gcccgcaagc gagttaccgg catcatccag ttccggactc cagactgcga
    10861 tggccatttc atgcggaaca atcgccacaa taccgccacc aacgccagat tttgccggta
    10921 gtcccacccg ccaggcaaac tcccccgcgt tctggtacat accgctggtc gccatcagcg
    10981 cgttaatttg ccgcgcctgc attggcgtca ccacaggttc atcaatatga atagctttcc
    11041 cctgattcgc cagaaagaca aacgtccggg ccagctctac acagctcatt ttcagagcgc
    11101 agtaatgaaa gtagttttgc agaacggttg tcacgtcatg atggaaattg ccaaacgact
    11161 tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc aaattcggaa cgcgctacca
    11221 ccgtatcgta ggaaatatca gacacaccac ttaagccgcg cacgacttcc agcatacgtt
    11281 gccgaggagc gcttaatcgc ccttgcaaca tatcgcagac caccagcgca ccggcattaa
    11341 taaacggatt acgcggaata ccctgctcca tttccagttg cactaaagaa ttgaacggcg
    11401 atccagacgg atctttgccg acgcgttgcc agatttcctc ttcggagtaa tgacgcatgg
    11461 cgacaacgag actcagcact ttggaaatag actgaatgga aaaacgttct tgcgcgtctc
    11521 cggcctgaaa aagctgtccg tcaacggtac agatagcaat ccccaatcgg gaaccgtcta
    11581 ccgtagccag cgccggaata taatccgcga ctttaccctg accaatgagc ggccgcactt
    11641 gccgcaagat gttttctaaa attgcattat ccatggcgac tgccactttc tactcctgga
    11701 ccgcaggtct gaaaagacct gcgagtatat cagagctgaa tatgtagcgt cagttccggt
    11761 ctttccacac cgtctggata ttacagaatt cgtgtaagcc aaaatgggaa agctcacgcc
    11821 caaagccact cttcttcacg ccaccaaagg cgactcgcgc atcgctggca caataaccat
    11881 tgataaacac cccaccgcat tccagacgtg ccgccatctg tctggcctgt gtttcgtcgg
    11941 tggtgaaaat ggtcgctgaa aggccgaact cactatcatt agccagttcc agcgcatgtt
    12001 ctgcatcttt cgcaatggtg attgccgcaa cggggccaaa catttcttcc cgaaacgcgg
    12061 tcatttctgg ggtaacattc gccagaaccg ttggcggata gtagttacct gccccagcaa
    12121 tcttttcccc tcccagtaac aaacgcgcac cctgcgccag tgttttttcc acctgatgat
    12181 gcagctcatc acgtaaatca aaacgagcca ttggtccgag agcgttctct ttatcacggg
    12241 gatcgcccat tttcaaggct gccgcagctg ccacaaaacg ttcggtaaat gccgaagcaa
    12301 ttccctcttc gatgataaag cgttttgccg ctgcacaaac ctgtccggta ttctgataac
    12361 gtccggctac cgccgctttc accgccagtt ccagatcggc atcgttaagc acaataaacg
    12421 gatccgaacc acccagttcc agtacgcatt ttttcagtgc cgctccagcc tgtgcgccaa
    12481 tagccgctcc agcacgaaca cttccggtca ctgtgacagc agcaatgcgc gagtctttaa
    12541 tcatcagact gacaccgtcg ttgtcggcat tcagccagcc atatacgcct tgtgggatac
    12601 ccgcatcttt aaacacctgg gcaatgagct gtgcacagcc catcacattc ggcgcatgtt
    12661 taagtaagta gccgttacca gcaagaatga tgggaacagc gccacgcatc acctgccata
    12721 acggaaaatt ccacggcata atcgccagaa tcgtccccaa cggtcgatac tcaataaccg
    12781 cctgctgatt ttccaccagc gtaggttctg ccttcagcat tgccggacca tgttctgcat
    12841 accagtcaca caaattcgcc gatttcgcca cttcagcgcg cgcctggttg attggtttgc
    12901 ccatttcacg ggtgatcatt tgcgccattt cttcgctacg ggcgcgcaga gccttaccga
    12961 tatcacgcag tttttcagca cgataatcta tatttgtctc gcgccagtcg cgaaagcctg
    13021 ctgccgccag ctgaagcgcg ttttcgatat cgtcaacacc agcccacggc agcacagaaa
    13081 gttgttcacc ggtggcagga tttatcgaaa ttgcatgagt tgccggagta atggtcatcg
    13141 gggtatctcc tttatgagtc atggtatgaa gatacgcaga tttactcttg ctttaaaatg
    13201 aataatatta agccacttat tcacgaatcg agaatgctat ggatctgacc caactggaga
    13261 tgttcaacgc ggttgccgaa gctggcagca ttacccaggc tgcagcaaaa gtgcatcgcg
    13321 tgccgtcgaa tctcactacc cgtctacgcc agctggaaac agaactgggg gttgatctgt
    13381 ttattcgcga gaatcagcgt ttacgtctct cgcctgccgg gcataacttt ttacgctata
    13441 gccaacaaat tctcacgtta gtggatgaag cgcggagcgt tgtcgctggc gatgagccgc
    13501 aaggtttatt ttctctggga tcgctggaaa gcaccgctgc agtgcgcatt ccggccacgc
    13561 tggcggaatt taaccgtcgt tatcccaaaa ttcagttttc actttccacc ggcccttccg
    13621 gcactatgct ggaaggtgtg ctggagggaa aactgaatgc ggcgtttatt gatggaccca
    13681 ttaaccatac tgccatcgac gggataccgg tataccgcga ggaactaatg atcgtcacgc
    13741 cacaaggaca tgcgccagta acccgtgcca gtcaggtgaa tggcagtaac atttatgcct
    13801 tccgcgccaa ttgttcgtat cgtcgccact tcgaaagctg gtttcatgct gacggtgccg
    13861 ctccgggaac tattcacgag atggagtctt atcacgggat gttggcctgt gtggtcgcag
    13921 gagcaggcat tgcgcttatt ccacgctcta tgctggaaag tatgccgggg catcaccagg
    13981 ttgaagcgtg gccgttagct gagcaatggc gttggttaac aacctggctg gtctggcgtc
    14041 gtggtgcgaa aacgcgtccg cttgaggcat ttattcaact gctggatgcg cctgactcgg
    14101 caaaacaggg ttaccaatga gctatttttg atagttcatg cggataatat gctctatata
    14161 gtgatgttcc gatgacttat gactatatgg ggcaaatatg gttacgccag taagcatcag
    14221 caattacata tctcttcctg atgattttcc cgttcgtaat attgcacctc aagtaaaaga
    14281 agaacaggcc cccttcctga tcgtcattaa aatgtgatgt taacaactct ctgatgcgtg
    14341 acagaacaac cgtcagagag atggaaatgc gatccagatc acaaatgcat tgtattcaca
    14401 tcattaaccg ttttaagatc atttcatcac tttttcgcaa ctcacccgat aatctgttat
    14461 gacaacaaac actgtttccc gcaaagtagc gtggctacgg gtcgttacgc tggcagtcgc
    14521 cgcctttatc ttcaatacca ccgaatttgt ccctgttggc ctgctctctg acattgcgca
    14581 tagttttcac atgcaaaccg ctcaggtcgg catcatgttg acaatttatg catgggtagt
    14641 ggcgctaatg tcattgcctt ttatgttaat gaccagccag gttgaacgcc gcaaattact
    14701 gatctgcctg tttgtggtgt ttattgccag ccacgtactg tcgtttttat cgtggagttt
    14761 taccgttctg gtgatcagtc gcattggtgt ggcttttgca catgcgattt tctggtcgat
    14821 tacggcgtct ctggcgatcc gtatggctcc ggccgggaag cgagcacagg cattgagttt
    14881 aattgccacc ggtacagcac tggcaatggt attaggttta cctctcgggc gcattgtggg
    14941 gcagtatttt ggctggcgaa tgaccttctt cgcgattggt atcggggcgc ttattaccct
    15001 tttgtgcctg attaagttac ttcccttgct gcccagtgag cattccggtt cattgaaaag
    15061 cctcccgcta ttgttccgcc gcccggcatt gatgagcatt tatttgttaa ctgtggtggt
    15121 tgtcaccgcc cattacacgg catacagcta tatcgagcct tttgtacaaa acattgcggg
    15181 attcagcgcc aactttgcca cggcattact gttattactc ggtggtgcgg gcattattgg
    15241 cagcttgatt ttcggtaaac tgggtaatca gtatgcgtct gcgttggtga gtacagcgat
    15301 tgcgctattg ctggtgtgcc tggcactgct gctacctgcg gcgaacagtg aaatacacct
    15361 cgggatgctg agtattttct gggggatcgc gatgatgatc atcgggcttg gtatgcaggt
    15421 taaagtgctg gcgctggcac cagatgccac cgacgtcgcg atggcgctat tctccgggat
    15481 atttaatatt ggaatcggcg cgggtgcgtt ggtaggtaat caggtgagtc tgcactggtc
    15541 aatgtcgatg attggttatg taggcgcggt gcctgctttt gctgcgttaa tatggtcaat
    15601 catcattttt cgccgctggc cagtgacact cgaagaacag acgcaatagt cgaaaggccc
    15661 attcgggcct tttttaatga tacgttttaa tgatttccag gatgccgtta ataataaact
    15721 gcacgcccat acataccagc aggaatccca tcagacggga gatcgcttca atgcccccct
    15781 tacccaccag ccgcataatt gcgccagagc tgcgtagact tccccacaaa ataaccgcca
    15841 ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca atcagcaaag gttgaactct
    15901 gacgcactgt ggaggccgag ctaataatca tcgctatggt tcccggaccc gcggtacttg
    15961 gcattgccag tggtacaaaa gcaatattgg cgctgggttc atcttccagc tcttccgact
    16021 tgcttttcgc ttccggtgaa tcaatcgctt tctgttgcgg aaagagcatc cgaaaaccga
    16081 taaacgcgac gattaagccg cctgcaattc gcagaccggg aatcgaaatg ccaaatgtat
    16141 ccatcaccag ttgcccggcg taatacgcca ccatcatgat ggcaaatacg tacaccgagg
    16201 ccatcaacga ctgacgatta cgttcagcac tgttcatgtt gcctgcaagg ccaagaaata
    16261 acgcgacagt tgttaatggg ttagctaacg gtagtaacac caccagcccc aggcctattg
    16321 ctttaaacaa atctaacatt ggtggttgtt atcctgtgta tctggtttat cagcgaaaag
    16381 tataaggggt aaacaacgat aaagtgtcac tctttagcta accttgcatc gcactgacca
    16441 aaacttgaac cgatttagca aaacgtggca tcggtcaatt cattcatttg acttatactt
    16501 gcctgggcaa tattatcccc tgcaactaat tacttgccag ggcaactaat gtgaaaagta
    16561 ccagcgatct gttcaatgaa attattccat tgggtcgctt aatccatatg gttaatcaga
    16621 agaaagatcg cctgcttaac gagtatctgt ctccgctgga tattaccgcg gcacagttta
    16681 aggtgctctg ctctatccgc tgcgcggcgt gtattactcc ggttgaactg aaaaaagtgt
    16741 tgtcggtcga cctgggagca ctgacccgta tgctggatcg cctggtctgt aaaggctggg
    16801 tggaaaggtt gccgaacccg aatgataagc gcggcgtact ggtaaaactt acccccagcg
    16861 gcgcggcaat atgtgaacaa tgccatcaat tagttggcca ggacctgcac caagaattaa
    16921 caaaaaacct gacggcggac gaagtggcaa cacttgagca tttgcttaag aaagtcctgc
    16981 cgtaaacaaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
    17041 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
    17101 agagcgttcg ggttactcta aatggcacct gcaacggatg tttaaaaaag aaaccggtca
    17161 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
    17221 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
    17281 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
    17341 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aacagctagt tgataacgtg
    17401 acaacgtcac tgaggcaatc atgaaaccac ttttatccgc aatagcagct gcgcttattc
    17461 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagca ggttgttact tcctgtgcca
    17521 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
    17581 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
    17641 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatctttttg aagtctcaaa
    17701 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
    17761 tttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgttttt
    17821 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggtgtg tagccaatta
    17881 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
    17941 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
    18001 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
    18061 gtagccctgc aggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
    18121 ccaaatctcg tggttcaaca taattcagtt gcgggttatc tggcgaaaat tgcgtagtaa
    18181 aagacatgta gtagctaaag ccgataaagt cgacacaacc tctggcaaga atcgcgttat
    18241 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
    18301 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtcg
    18361 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatgc
    18421 agccgatatt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
    18481 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctcta
    18541 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
    18601 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
    18661 ttgatgcgaa gcggatgaaa aagtcgatca gtttacggtt tcgccagcca ccatattttg
    18721 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggctcc attccctgct
    18781 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
    18841 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
    18901 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
    18961 tgggataatt aagtccgtcg ataacgcctt ctgtcacttc acgcggcaca ccgtgagcgc
    19021 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
    19081 actgatgcgc ggctaccgcg ccgccccata aaaaaccttt tttaaatcct gacatacaca
    19141 actccttaac taaaatagat taccaccaga cttccgcctg gatgccggcc ataaactcgt
    19201 ctttgctgtt ctcattaaac ttaaaattcg ataattcgtt atccagaata ttgatatacg
    19261 tgccatagaa gcgaatttct ggtcgtgaac ctaaaatgct ttcacccact ttcaatgcat
    19321 gccagagtgt cgttttataa gccgattcat ttagtgttac
//
LOCUS       DADSZD010000082        18907 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.082, whole genome shotgun sequence.
ACCESSION   DADSZD010000082 DADSZD010000000
VERSION     DADSZD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.082"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            53..1039
                     /locus_tag="J1Z28_002619"
     CDS             53..1039
                     /locus_tag="J1Z28_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBA7717442.1"
                     /translation="MSELIATAASSINAFTLAKRVAAFNVPVLIQGETGAGKECVAKY
                     IHTVAFGENDNAPYIGVNCAAIPENMLESTLFGYDKGAFTGAIASVPGKMELANNGTL
                     LLDEIGDMPLALQAKILRVLQEQQVERLGSNRQIKLNFRLIACTNKNLEQDVTAGRFR
                     EDLYYRLAVIPITMPPLRERLNDIIPLAESFIKKYATVLVKNITLSESTRRALLNYRW
                     PGNVRQLENAIQRGMILNRDGVIYPDALGLPETDIADHSELQWPVQPAVHIAETSDLG
                     QHGRSAQYQYIADLMRKYQGNRSKIADLLGITPRALRYRLASMRKQGIEVFS"
     gene            1054..1395
                     /locus_tag="J1Z28_002620"
     CDS             1054..1395
                     /locus_tag="J1Z28_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBA7717443.1"
                     /translation="MSITTINSTMQAQIMQDVQRMQANAQAPVLPAMTFSSTDPDVSF
                     NRIMYGALGHVDQFQQVAEQQQTAVDTGKSDDLAGAMIASQQASLSFSALVQVRNKIA
                     TGFNDLMSMSI"
     gene            1400..3046
                     /gene="fliF"
                     /locus_tag="J1Z28_002621"
     CDS             1400..3046
                     /gene="fliF"
                     /locus_tag="J1Z28_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBA7717444.1"
                     /translation="MNAQIKKLTQAFPAFRLRLVDNKRWALMAGVGLAVAATAIIVSV
                     LWTGNRGYVSLYGRQENLPVSQIVTVLDGEKLTYRIDPQSGQILVPEDALSKTRMTLA
                     AKGVQAILPSGYELMDKDEVLGSSQFVQNVRYKRSLEGELAQSIMSLDAVESARVHLA
                     LNEESSFVVSDEPQNSASVVVRLHYGAKLNMDQVNAIVHLVSGSIPGLQASKVSVVDQ
                     AGNLLTDGIGAGEAVSAATRKRDQILKDIQDKTRASVANVLDSLVGTGNYRVSVMPDL
                     DLSTIDETQEHYGDAPKINREESVLDSDTNQVAMGVPGSLSNRPPIAANQMSNGTEEN
                     RSPEALSKHSESKRDYSYDRSVQHIQHPGFAVKRLNVAVVLNQNAPALKNWKPEQTTQ
                     LTALLNNAAGIDAQRGDNLSLSLLNFVPQAVPVEPVIPLWKDDSVLAWVRLIGCGLLA
                     LLLLFFVVRPLMKRLTAVRAPVITPEPEAVSEPWIAMPEEERKNVDLPSLPGDDSLPS
                     QSSGLEVKLEFLQKLAMSDTDRVAEVLRQWITSNERIDNK"
     gene            3024..4034
                     /locus_tag="J1Z28_002622"
     CDS             3024..4034
                     /locus_tag="J1Z28_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006685955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBA7717445.1"
                     /translation="MSELTTNKSNSYLEQAAILLLCLGEEAAATVMQKLSREEVVRLS
                     ENMARLSGVKTSMARKVINNFFDEFREQSGINGASRSMLQGILNKALGTEIASSVING
                     IYGDEIRSRMARLQWVEPRQLAMLISEEHLQLQAVFLAFLTPEISAAVLSYLNESVQN
                     EILYRVAKLNDVNRDVVDELDRLIERGLSVLSEHGSKVKGIKQAADIVNRFQGNQQVI
                     LDQMRERDEEVLGQIQDEMYDFFILSRQNEEVRRRLLDEVPMEDWAVALKGTEALLRR
                     SIYAVMPKRQAQQLEAITARLGPVPVSRIEQIRREIMGIARELEEAGEIQLQLFAERT
                     AE"
     gene            4038..4748
                     /locus_tag="J1Z28_002623"
     CDS             4038..4748
                     /locus_tag="J1Z28_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein H"
                     /protein_id="HBA7717446.1"
                     /translation="MTIETLRGRYRLHRFPPRQRAVTGEPLTASISAADYQRQLMDGF
                     QEGLQKGFAQGMTEGQEQGFSEGHQQGFAEGRRQGYTEGSLAGQQEGCKQFVEAAQPL
                     EAITGKVNDFLAHIERKQREDLLQLVEKVTRQVIRCELALQPTQLLALVEEALAAFPA
                     MPETLQVMLSTEEFNRLRDAVPEKVSEWGLTPSPDLPPGECRVITDKSELDIGCEHRL
                     EQCMTALQETLTPESQGE"
     gene            4741..6081
                     /gene="fliI"
                     /locus_tag="J1Z28_002624"
     CDS             4741..6081
                     /gene="fliI"
                     /locus_tag="J1Z28_002624"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HBA7717447.1"
                     /translation="MSDFSAFDNALRSLESIPLARVAGRLVRLNGILLESVGCPLMTG
                     QLCRIESANHTLIDAQAVGFNRDITYLMPFKQPVGLMAGARVFPEEKAHDILIGESWL
                     GRVVNGLGEPLDGKGRLNGNDLLPPLPPSVNPLTRRSVDEPLDVGVKAINGLLTIGKG
                     QRVGLMAGSGVGKSVLLGMITRQTKADIVVVGLIGERGREVKEFIDHSLGADGLAKSI
                     VVVAPADESPLMRLKATELCHSIAAWFRDRGHHVLLLVDSLTRYAMAQREIALSLGEP
                     PATKGYPPSAFGMIPKLVESAGNSESAGSMTAIYTVLAEGDDQQDPIVDCARAVLDGH
                     IVLTRKLAEAGHYPAIDIGQSISRCMNQVTSPEHQQSARAMKQNYAAYMEIKPLIPLG
                     GYVAGADATVDKAVKMFPAIERFLRQEMREPASLELVQSRLQILFPIAKKAEGQ"
     gene            6084..6518
                     /gene="fliJ"
                     /locus_tag="J1Z28_002625"
     CDS             6084..6518
                     /gene="fliJ"
                     /locus_tag="J1Z28_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HBA7717448.1"
                     /translation="MRQIIDTLAQLQRLRDKSVKDKTVELAKQKQICAGYDNNIKALG
                     YLVEKTSAGAAASVESLKNVSGYKGTLRKVIAWQEQEKTLANIKATRMQKNLTAAACE
                     EKVVALTLDDKRREQQESATAKAQKAADDIAVQCWLRHKLAE"
     gene            6521..6925
                     /locus_tag="J1Z28_002626"
     CDS             6521..6925
                     /locus_tag="J1Z28_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HBA7717449.1"
                     /translation="MKTVITFGTFDVFHVGHLRLLQRARTLGERLLVGVSSDALNIAK
                     KGRAPVYPQDDRMSIIAGLACVDGVFLEESLEQKAEYLRGYSADILVMGDDWAGKFDD
                     FSCICEVVYFPRTPSVSTTGIIEVIRGNYIKY"
     gene            complement(7035..9473)
                     /locus_tag="J1Z28_002627"
     CDS             complement(7035..9473)
                     /locus_tag="J1Z28_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA7717450.1"
                     /translation="MQTLIKTLATARVKPFVSVVTPTWNRGAFLPYLLYMYRYQDYPA
                     DRRELIILDDSPQSHQHIIDRLTNGTPESFNIRYIHHPEKLPLGKKRNMLNELARGEY
                     IFCMDDDDYYPPDKISYSIAMMQKHRALISGSDQIPIWYSHINRIFKSRSFGPHNILN
                     GTFCYHRNYLKKHRYDDDCNLGEEKSFTNNFSVNPLQLPGERTILCISHSHNTFDKDF
                     ILGASTPVNAALTDIVSDPLLRNAYLGLHNATHYQAIQHQAIDQIVLLNLDKRPDRLQ
                     QIREELTLLHIPPEKITRLAASENENGQRGRQQSHLQALHLAQQHGWQNYLLLEDDAV
                     ILKQEKHIQVLNALLASLAKIPWQVMILGGEISQGTMLKSLPGLVHARDCRKVCAYLV
                     NSRYYPQLAQQMNNDEHSLEDGWQPLLRTDKWLACYPSLCYQRPGFSDIEKKATDNIN
                     HYFNKLPVATKPSTLPIADTIGFFMETSFHYALYRPIITALQAQGQSCTLVINDRVFK
                     PFLDEMLATLKSIDDPQLKGMRLSEMQAHGQRVKCLVSPYHTPALNGLSTLNIRAMYG
                     LAKETWNHADWNRFYQRILCYSHYSQRALAHFGSAKVVGNPRFDAWHNGTFDRALPEN
                     IQPDSRKPTVLYAPTFGALSSLPHWAEKLGRLSGDVNLMCKLHHGTCSRPEEAASLAL
                     ARRHIKQRTDSARHTLALLAKADYVLTDNSGFIFDAIHVDKRLILLDFPGMTELLDGE
                     KSYSTAESADQRIREILPVAHDVAELRYLLSEVFDWGAVQTRLTEIRHHYCDAFMDGK
                     SGERAALVIVEALG"
     gene            complement(9520..10452)
                     /locus_tag="J1Z28_002628"
     CDS             complement(9520..10452)
                     /locus_tag="J1Z28_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-N-methylase"
                     /protein_id="HBA7717451.1"
                     /translation="MSFIECYEPEYVRNFMARHPDSPLYVRKVWKNEIRLSLMLTEPE
                     SCEAVLNDACAFDLQLTYRPVEENAAELSARDAIVNQVILSTLTLPDLTPELSLYAVG
                     IVLSRASKMPGRDGDILARLTTLPQALADHAQKGTLQAQFAQLPPVPQLARQLMTLLG
                     SFTFDWSILPESPRKASLPLQVTLLTLHDANSEALLQQQLQAQWQTIWQQHFATAPWM
                     MRNWLIYRVYHEVIGQADGADYCPLVCDFYLLRTLISLWTLDGSSLRQEDIFALFAMF
                     ERWRASENAVLIRQQIQAECSADPLLYAFSLLSC"
     gene            complement(10580..11008)
                     /locus_tag="J1Z28_002629"
     CDS             complement(10580..11008)
                     /locus_tag="J1Z28_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBA7717452.1"
                     /translation="MSIRLQQVKALLQGIRDDDALYDSLRELLQRQRICMIRRASEDL
                     LAVNDEITQHYEQLHGHSYQRHSLLKMLGVSVNRDGLAQVFAWLPAVQKAAAQQLWQR
                     LEQKAERCKAYNDKNGELLIRQYEFIQAFLGSEADFLYQG"
     gene            complement(11021..11299)
                     /gene="flgM"
                     /locus_tag="J1Z28_002630"
     CDS             complement(11021..11299)
                     /gene="flgM"
                     /locus_tag="J1Z28_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBA7717453.1"
                     /translation="MKITPTMPGNHPTATMAGSSQPRKSTATTATTLSADDITQAGLQ
                     SAQQTLNDQQESDIDSDKVAQMQAMLASGSLQVDTEQLASDMLSFFQN"
     gene            complement(11380..12117)
                     /gene="flgA"
                     /locus_tag="J1Z28_002631"
     CDS             complement(11380..12117)
                     /gene="flgA"
                     /locus_tag="J1Z28_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBA7717454.1"
                     /translation="MSFQFCRRKFYFLLALALPGYAAVHPVQHSAREQVNAQVLNAAS
                     QKIESLAQQRRWHDYRYTFKVYIPSQIATAAPCTKTPGVTLTSPGDIALNRMNFTVSC
                     PQSWQMNVAVRPDVLVPVVMAKSLVARDTPLTANDVELRPYNVSAQRREVLMEPNDAI
                     GFSSKHALQPGRPITKEELISPVLVERDQPVMIVYQSAGITASMPGVALKNGRKGEMV
                     KVRNASSQRVISAMVAESGVVTTVSAE"
     gene            12199..12534
                     /gene="flgB"
                     /locus_tag="J1Z28_002632"
     CDS             12199..12534
                     /gene="flgB"
                     /locus_tag="J1Z28_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838822.1"
                     /note="with FlgF and C makes up the proximal portion of
                     the flagellar basal body rod; Yersinia has 2 copies of
                     flgB and other flagellar genes; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBA7717455.1"
                     /translation="MGISFQQALGVHPQAVKLRLERTELLTANLANVDTPNFKAKDID
                     FAREMQRANNAAVDVQYRVPMQPSEDGNTVELNSEQARFSQNSMDYQSSLTFLNLQIS
                     GIREAIEGK"
     gene            12537..12968
                     /gene="flgC"
                     /locus_tag="J1Z28_002633"
     CDS             12537..12968
                     /gene="flgC"
                     /locus_tag="J1Z28_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBA7717456.1"
                     /translation="MSFTDIYQISGSAMTAQTLRLNTVASNLANANAPASSEAQAYKA
                     RSPVFAAVYHHSLLAGTHRHAIDGASVQVQDVLQTGGAVKRYEPHSPLADANGDVWYP
                     DVNVVEQMADMMSASRDFETNVDVLNNVKSMQQSLLKLGEA"
     gene            12968..13681
                     /locus_tag="J1Z28_002634"
     CDS             12968..13681
                     /locus_tag="J1Z28_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001508388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping protein"
                     /protein_id="HBA7717457.1"
                     /translation="MNTLALNAQSPQPTATAESNNVAASDTTSSSDSSPVDTFLTLFV
                     AEIQNQDPTDPTDATEYIDQLSSMAQVAMMEEMSVQANTNAVLMSNIQVMALGNLVGD
                     DIMVQTTALQVSDQTINGRATLDDACTTADLHFTDAAGDDYTVSLIPEGSSSVGPGQV
                     DFSINPADYGIPPGDYDVSVVTNTGEEEVPIEVSGEVEDVRIPLDGSSPVLNVAGVGE
                     VPFTMISQFGVPDADSDAA"
     gene            13841..15043
                     /gene="flaE"
                     /locus_tag="J1Z28_002635"
     CDS             13841..15043
                     /gene="flaE"
                     /locus_tag="J1Z28_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein FlaE"
                     /protein_id="HBA7717458.1"
                     /translation="MSYEIAATGLNAVNEQLDGISNNIANAGTVGYKSMTTQFSAMYA
                     GSQAMGVSVAGTAQSISRGGSLVSTGNALDLAINDDGFFVTCDSAGNISYTRAGSFET
                     DKNGYIVNASGAYLQGYPVDDTGTLQTGTVTDIQIKTGNIPAQASSSLTFTANFDASD
                     AAIDRTTVPFDATNSSSYTDSYTTTVYDSLGNEHSVCQYFTKTSDNTWEVQYTFDGQQ
                     QTGVPATTLTFDPNTGKLTSPNTPQTIEFQTDAAAPIDLTVDYSTCTQYGSEFSVTTN
                     AADGYASATQNGVQVDDDGKVYATYSNGERMLQGQVVLATFPNENGLEAVSGTVWVQT
                     GESGTPLIGVPGSGTCGTLSSGVLESSNVDITSELVNLMTAQRNYQANTKVIATSTQL
                     DDALFQAM"
     gene            15043..15780
                     /locus_tag="J1Z28_002636"
     CDS             15043..15780
                     /locus_tag="J1Z28_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HBA7717459.1"
                     /translation="MDRLIYTALSGASQTLYEQQISANNLANVNTNGFRADMAMATND
                     KVKGGGFDTRYMAQEGASGVNDSTGVAEKTERPLDVAIQGSGYIAVQDKNGNEVYTRN
                     GNIQQDDQGQLTIDGNLVLGDNGPIILPPNAIASFGSDGTLSVTPDDGDVTATMDIDR
                     LKLVDIPVANLAKNPEGMLVTADGVPAQRDENIKVSGGFLEGSNVSAVSEMMSSIAMN
                     RQFEAQIKMMKTAEDISDAGNRLLRGS"
     gene            15859..16644
                     /gene="flgG"
                     /locus_tag="J1Z28_002637"
     CDS             15859..16644
                     /gene="flgG"
                     /locus_tag="J1Z28_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBA7717460.1"
                     /translation="MNAALWISKTGLSAQDAEMSAIANNIANVNTTGFKRDRVMFQDL
                     FYQTQEAPGAMLDQNNIMPTGLQFGSGVRIVGTQKTFTEGNVETTDNAMNVAIMGQGF
                     LQVQKANGDIAYTRDGNLQVNADGVLTNSQGLPLQPEIDVPAGATNVAFGEDGTVTAI
                     LPGDSDVTELGQLTLVNFANPAGLSAEGDNLYLETAASGQPTEGVPGEDGLGTLQDNA
                     LEGSNVDIVNEMVAMITVQRAYEMNAKMVSAADDMLQYISQTL"
     gene            16727..17392
                     /gene="flgH"
                     /locus_tag="J1Z28_002638"
     CDS             16727..17392
                     /gene="flgH"
                     /locus_tag="J1Z28_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBA7717461.1"
                     /translation="MKNYLWLVALLPLLSGCESQAILVKKDDAYFAPPTTEAPPPADG
                     RAGGVFETGYNWSLTADRRAYRVGDILTVILEESTQSSKQAKTNFGKSNTVDIGAPTI
                     FGHTKDKLSGSIDANRDFDGSATSQQQNSLRGEITVSVHAVQPNGILEIRGEKWLTLN
                     QGDEYIRLSGLVRADDIQNDNSVSSQRIADARISYAGRGALSDANAAGWLTRLFNHPL
                     FPI"
     gene            17407..18507
                     /locus_tag="J1Z28_002639"
     CDS             17407..18507
                     /locus_tag="J1Z28_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBA7717462.1"
                     /translation="MQNWIKAAAIAVSLTLPGVALAQSLESLVNVQGVRENQLVGYSL
                     VVGLDGTGDKNQVKFTNQTITNMLRQFGVQLPNKIDPKVKNVAAVAVSATLPPMYSRG
                     QTIDVTVSSIGDAKSIRGGTLLLTQLHGADGEVYALAQGSVVVGGMNATGASGSSVTV
                     NTPTAGLIPNGATVEREIPSDFQMGDTITLNLKRPSFKDANNIAAAINASFGGIATAQ
                     SSTNVSVRAPTSPGARVAFMSQLDDVQVQAEKIRARVVFNSRTGTVVMGDGVALHAAA
                     VSHGSLTVSINETSNVSQPNAFAGGRTAVTPQSNIAVNHARPGVVSLPESSSLKTLVN
                     ALNSLGATPDDIMSILQALHEAGALDADLEVI"
     gene            18507..18806
                     /locus_tag="J1Z28_002640"
     CDS             18507..18806
                     /locus_tag="J1Z28_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HBA7717463.1"
                     /translation="MKVNASGGIDGSDALMGPKVQTNDIKQAAEQFEAIFLRNMLKEM
                     RKTNELFDSKDNPFNSDSVRMMQGFYDDELCNTLAQQHGIGIAAMIVKQLSPKHK"
BASE COUNT         4341 a         5107 c         5566 g         3893 t
ORIGIN      
        1 tgatacgctt cagaacatcc tgttttctgt cgctactgat atcgttcacc ctatgtctga
       61 acttattgca actgccgctt ccagcattaa cgcttttact ctggcaaagc gtgtcgccgc
      121 ctttaacgta ccggtgctca ttcagggcga aaccggcgcg gggaaagagt gcgtggcgaa
      181 atatattcac accgtagcct ttggtgaaaa tgataacgcg ccctatatcg gcgtgaactg
      241 cgcggcgatc ccggaaaata tgctggaatc gaccttattt ggctacgaca aaggtgcatt
      301 taccggcgca attgccagcg tgccgggaaa aatggaactg gctaataacg gcaccttatt
      361 gctcgatgaa attggcgata tgccgctggc attacaggcc aaaatattac gcgtattgca
      421 ggaacagcag gttgagcgat taggcagcaa ccgacaaata aaactcaatt ttcgcctgat
      481 tgcctgcacc aataaaaatc tggaacagga tgtgactgct ggacgttttc gtgaagatct
      541 ttattatcgt ctggcggtta ttcctataac catgccgcca ctgcgtgaac gtctgaacga
      601 tattattccg ctggcagagt cgtttattaa aaaatatgcc acggtgctgg tgaaaaatat
      661 caccctttca gaatctaccc gccgggcgct acttaattac cgctggcccg gaaatgttcg
      721 ccaactggaa aacgccattc agcgaggaat gatcttaaac cgcgacggcg tgatttaccc
      781 ggatgcctta ggcctgccgg aaactgacat tgccgaccac agcgaactgc aatggcctgt
      841 tcagcccgcc gtccacattg ccgaaaccag cgatttaggc cagcacggac gaagtgcgca
      901 gtatcaatat atcgccgacc tgatgcgtaa atatcagggc aaccgcagca aaatcgccga
      961 cctgttaggc attaccccgc gcgcactgcg ctatcggctg gcctccatgc gcaagcaagg
     1021 tatcgaagtt ttctcctgac ccggaagtta aaaatgagca ttaccacaat caacagcacc
     1081 atgcaggcgc aaatcatgca ggacgtgcag cggatgcagg ccaacgccca ggcgccggtg
     1141 ctacccgcca tgaccttcag ttccacagac ccggacgtgt cgtttaaccg cattatgtac
     1201 ggcgcgcttg gtcacgttga tcagttccag caggtggcgg agcagcagca aacggctgtc
     1261 gacacgggca aaagtgatga cctggcaggt gcgatgatcg ccagccagca ggcgtcgctc
     1321 tctttctccg cgctggtgca ggtgcgcaat aaaatcgcca ccgggtttaa cgacctgatg
     1381 agtatgtcga tctaacccta tgaatgcaca aataaagaag ttaacccagg cgtttccggc
     1441 gtttcgactg cgtctcgtag ataacaaacg ctgggcgttg atggcgggag tggggcttgc
     1501 cgtggcggcg acggcgatta tcgtcagcgt gctgtggacc ggtaatcgcg gctacgtttc
     1561 gctctacgga cgccaggaaa atctgcccgt gtcgcagatt gtcacggtgc tggacggcga
     1621 aaagctcacc taccgcatcg acccgcagag cgggcagatt ctggtgccgg aagatgcact
     1681 gtcgaaaacg cgcatgaccc ttgccgcgaa aggcgtgcag gcgatattgc ccagcggcta
     1741 cgagctgatg gataaagacg aagtgctcgg cagcagtcag ttcgtgcaga acgtgcgcta
     1801 taaacgcagt ctcgaagggg agctggcgca gagcatcatg tcgctggacg cggtggaaag
     1861 cgcgcgggtg catctggcgc tcaacgaaga aagctcgttt gtggtcagcg atgagccgca
     1921 aaacagcgcc tcggtggtgg tgcgcttgca ttacggcgcg aagctgaata tggaccaggt
     1981 gaacgccatt gtgcatctgg tgtccggcag tattccgggg ctacaggcca gtaaagtcag
     2041 cgtggtcgat caggcgggaa atctgctgac cgacggcatt ggcgcaggcg aggcggtttc
     2101 tgccgcgacc cgtaaacgcg atcagatcct caaagatatt caggacaaaa cccgtgccag
     2161 cgtggcgaac gtgctggatt cgctggttgg cacggggaat taccgcgtca gtgtgatgcc
     2221 ggatctcgac ctcagcacca tcgacgaaac ccaggaacac tacggtgacg cgccgaaaat
     2281 caaccgcgaa gagagcgtgc tggacagcga caccaatcag gtggcgatgg gcgtgcctgg
     2341 ttcgctcagt aaccgtccgc cgattgcagc gaatcagatg agcaacggta cggaagaaaa
     2401 ccgctcgccg gaagcgcttt ccaaacacag cgaaagtaag cgcgactact cttacgaccg
     2461 cagcgtccag catattcagc atcccggctt cgcggtgaaa cgcctcaacg tggcggtggt
     2521 actcaatcaa aacgctccgg cgctgaaaaa ctggaagccg gagcaaacca cgcaactgac
     2581 cgcgttgctg aacaatgccg ccgggatcga cgcccagcgt ggtgacaatc tctccttatc
     2641 gttactcaac tttgtaccgc aggcggtgcc tgtcgaaccg gttattccac tgtggaaaga
     2701 tgacagcgta ctggcctggg tgcggctgat tggctgcggc ctgctggcgc tgttgctgct
     2761 gtttttcgtg gtgcgtccgt tgatgaaacg gctgacggcg gtacgtgcgc cggttatcac
     2821 accagaaccg gaagccgtca gtgaaccgtg gattgccatg ccggaagagg agcgcaaaaa
     2881 cgtcgacctg ccgtcgctac ccggcgatga cagcctgccg tcacaaagtt ctggcctcga
     2941 agtgaaactc gagttcctgc aaaaactggc catgagcgac accgatcgcg tagccgaagt
     3001 tctcagacaa tggatcacca gcaatgagcg aattgacaac aaataagagc aacagctacc
     3061 tggaacaggc ggcgatcctg ttgctgtgtc tgggcgaaga ggcggcggcg acggtgatgc
     3121 aaaaactcag ccgcgaagaa gtggtgcgcc tgagcgaaaa catggcgcgg ctttccggcg
     3181 tcaaaaccag catggcgcgc aaggtgatca ataacttctt tgatgagttc cgcgaacaga
     3241 gcgggatcaa cggcgcatca cgctccatgt tgcagggcat tcttaacaaa gcgctgggca
     3301 ccgagatcgc cagtagcgtg atcaacggca tctatggcga tgagatccgc tcgcggatgg
     3361 cgcgcctgca atgggtggaa ccacgccagt tggcgatgct gatttccgaa gaacatctgc
     3421 aattgcaggc ggtgttcctc gcgtttctga cgccggaaat ctcggcagcg gtgctctctt
     3481 atctcaacga atcggtgcaa aacgaaattc tctaccgggt ggcgaaactc aacgacgtca
     3541 accgcgacgt ggtggatgag ctggaccgcc tgattgaacg tggcctgtcg gtgctttcgg
     3601 agcacggctc gaaggtgaag ggcatcaaac aggcggcgga tatcgtcaac cgcttccagg
     3661 gcaatcagca ggtgatcctc gaccagatgc gcgaacgtga cgaagaggtg ttggggcaga
     3721 tccaggatga gatgtacgac ttctttatcc tcagccgcca gaacgaagaa gtgcgtcggc
     3781 gtctgcttga cgaagtgccg atggaagact gggcggtggc gctgaaaggc accgaagccc
     3841 tgctgcgccg ctcgatttac gcggtaatgc caaaacgtca ggcgcagcaa ctggaagcga
     3901 tcaccgcgcg tctgggtccg gtgccggtca gccgtatcga acagatccgc cgcgaaatca
     3961 tgggcatcgc ccgtgagctg gaagaagcgg gcgaaattca gctacagctc tttgcggaac
     4021 ggacggcgga gtaattcatg accattgaaa cactgcgcgg gcgctatcgc ctgcaccgct
     4081 tcccgccgcg ccagcgtgcg gtgaccggag aaccgcttac tgccagtatc agcgccgccg
     4141 attaccagcg tcagttgatg gacggttttc aggaagggct gcaaaaaggg tttgctcagg
     4201 gaatgaccga aggtcaggag cagggcttta gcgaaggtca tcaacaaggt tttgccgagg
     4261 ggcgacgtca gggctacacc gaaggcagcc tcgccgggca gcaggaagga tgcaaacagt
     4321 ttgttgaggc ggcgcagccg ctggaagcca tcaccggtaa agtgaacgac ttcctcgcgc
     4381 atattgaacg taaacagcgg gaagatttgc tgcaactggt ggagaaagtc acccgtcagg
     4441 tgatccgctg cgaactggcg ctgcaaccga cgcagttact ggcgctggtg gaagaggcgc
     4501 tggcggcctt cccggcgatg ccggagaccc tgcaagtgat gctgagtaca gaagagttta
     4561 accgcctgcg cgacgcggtg ccggaaaaag tcagcgaatg ggggctgacg ccttcgccgg
     4621 atctgccgcc aggcgaatgc cgagtgatta ccgacaaatc tgaactggat atcggctgtg
     4681 agcatcgcct tgaacagtgc atgaccgcgc tgcaagagac gctaacgccg gagtcgcagg
     4741 gtgagtgatt tttctgcttt cgataacgcc ctgcgctcgc tggagtcgat cccgctggcc
     4801 cgcgtcgccg ggcggctggt gcgcctgaac ggcattctgc tggagagcgt cggctgcccg
     4861 ctaatgaccg ggcaactgtg ccgcatcgaa agcgccaatc acacgctgat cgacgcgcag
     4921 gccgtgggct ttaatcgcga catcacctac ttaatgccgt ttaaacagcc ggtcggcctg
     4981 atggcaggcg cacgcgtctt cccggaagaa aaagcccacg acatcctgat tggcgaaagc
     5041 tggttaggcc gcgtggttaa cggtctcggc gaaccgctgg atggcaaagg ccgcctgaac
     5101 ggcaacgacc ttctgccgcc gctgccaccc tcggtcaatc ccttaacccg ccgttcagtg
     5161 gacgaaccgc tggacgtggg cgtgaaagcg attaacggcc tgctgaccat cggcaaaggc
     5221 cagcgtgtcg ggctgatggc gggcagcggc gtcgggaaaa gcgtgctgct ggggatgatc
     5281 acccgccaga ccaaagccga cattgtggtg gtcgggttga ttggcgaacg cgggcgcgaa
     5341 gtgaaagagt ttattgacca ttcgctgggc gcggacgggc tggcaaagtc tatcgttgtt
     5401 gtcgcgcctg ccgatgaatc gccgctgatg cgcctgaaag ccaccgaact gtgccactcc
     5461 atcgccgcct ggtttcgcga ccgcgggcat cacgttttac tgctggtgga ttccctgacc
     5521 cgctacgcca tggctcagcg ggaaattgct ctttcactcg gcgaaccgcc cgccaccaaa
     5581 ggctatccgc catcggcatt cggcatgatc cccaaactgg tcgagagcgc aggcaacagc
     5641 gaaagcgcag gatcgatgac cgccatctac accgtgctgg cggaaggcga cgaccagcaa
     5701 gatccaatag tcgactgcgc ccgcgcagtg ctggacggac atatcgtatt aacccgcaaa
     5761 ctggcggaag cggggcacta tccggctatt gatatcggcc agtcgatcag ccgctgcatg
     5821 aaccaggtca ccagcccgga acatcagcaa tccgcccgtg cgatgaaaca aaactacgcc
     5881 gcctatatgg agatcaaacc gctgatcccg ctcgggggct acgtggcagg tgcagacgcc
     5941 accgtcgaca aagcggtgaa aatgttcccg gctatcgagc ggtttttgcg ccaggagatg
     6001 cgcgaacccg cttccctcga actggtgcaa agccgcctgc aaatcctctt tcccatcgcc
     6061 aaaaaggcag aagggcaata accatgcgac aaatcatcga cactctggcg cagttgcagc
     6121 gcctgcggga taaatcggtc aaagacaaaa ccgtcgaact ggcaaagcaa aaacagatct
     6181 gcgcgggcta tgacaacaat atcaaagcgt taggctacct ggtggagaaa acgtctgcgg
     6241 gcgcggcggc gtcggtggaa tcgctgaaaa acgtcagcgg ctacaaaggt acgctgcgca
     6301 aggtgatcgc ctggcaggag caggaaaaaa cgctggctaa catcaaagcc acgcgaatgc
     6361 agaaaaacct gacggcggcg gcgtgcgaag agaaagtggt ggccctgacg ctggacgaca
     6421 aacgccgcga gcagcaagaa agtgccacgg cgaaagcgca gaaagcggcg gatgacatcg
     6481 ccgttcagtg ctggctgcgg cataagttag cggagtagcc atgaaaacgg tcatcacctt
     6541 cggcaccttc gatgtgtttc atgtcggtca tctgcgcctt ttacaacgcg cccgcacgct
     6601 gggcgaacgg ctgctggtcg gcgtatcgtc cgacgcctta aatatcgcca aaaagggcag
     6661 agcaccggtt taccctcagg atgaccgcat gtcgattatc gccgggctgg cgtgcgtgga
     6721 tggcgtattt ctggaggagt cactggagca aaaagcggaa tatttgcggg ggtacagtgc
     6781 ggatattttg gtgatggggg atgactgggc agggaagttt gacgatttct cgtgtatctg
     6841 tgaggtggtc tattttcccc gtacgccgtc ggtatcgacg acggggatca ttgaggtgat
     6901 tagggggaat tatataaagt actaacacag agatgtttgt ttctttccgg atgcgacgta
     6961 aacgccttat ctgcctacaa gacgtgatcg atccgtaggc ctgataagcg aagcgcatca
     7021 ggcacttgcg cggtttaccc caacgcctcc acaatcacca acgccgctcg ttctcccgac
     7081 ttgccatcca taaacgcatc gcaatagtga tggcgaattt ccgtaagccg cgtctgcacc
     7141 gccccccagt caaatacctc tgacaataag taacgcagtt ccgcaacatc atgagccacc
     7201 ggcaatattt cgcggattcg ctggtcggcg ctctctgccg tggagtagct tttctcaccg
     7261 tcgagcagtt ccgtcatgcc aggaaagtcg agcaggatca accgcttatc aacgtgaata
     7321 gcatcgaaaa taaagccgct gttatcggtc agcacgtaat cggcctttgc cagtagcgcc
     7381 agcgtgtggc gggcagagtc ggtgcgctgt tttatatgcc tgcgcgccag tgccagcgat
     7441 gccgcttctt ccgggcgcga gcaggtgccg tggtgcagtt tgcacatcag gttcacatca
     7501 ccgcttaagc ggcccaattt ttctgcccag tggggcaggg agcttaatgc gccaaacgtg
     7561 ggggcgtaga gcaccgtagg tttacgggaa tcgggttgga tattttccgg cagagcgcgg
     7621 tcaaacgtgc cgttatgcca ggcgtcgaag cgcggattgc cgaccacttt tgcgctgccg
     7681 aaatgcgcca gcgcccgctg gctgtagtgg ctgtaacaca aaatgcgctg ataaaaacgg
     7741 ttccagtcgg cgtggttcca cgtttctttg gctaagccgt acatggcgcg aatgttcagg
     7801 gttgaaaggc cattgagcgc aggcgtgtga taggggctga ccaggcattt tactcgctga
     7861 ccgtgtgcct gcatttcaga taagcgcatt cctttgagct gcggatcgtc gatgcttttt
     7921 agcgtcgcca gcatttcatc cagaaatggt ttaaatacgc ggtcattgat caccagcgta
     7981 cagctctgcc cctgcgcctg cagggcggtg atgattgggc ggtagagcgc gtagtggaat
     8041 gaggtttcca taaaaaaacc gatggtatcg gcgataggta atgtagatgg cttggtggct
     8101 acgggtaact tattgaagta gtggttaatg ttatcagtcg cttttttctc gatatcacta
     8161 aagcccggtc gctgataaca aaggctcggg taacaggcca gccatttgtc ggtgcgcagc
     8221 agcggctgcc agccgtcttc cagcgaatgc tcatcgttgt tcatctgctg cgccagctgc
     8281 gggtagtagc ggctgttcac cagataagcg cacactttgc ggcagtcccg cgcgtgcacc
     8341 agcccaggca aactttttag catcgttccc tgcgaaattt cgccgccgag gatcatcacc
     8401 tgccagggaa ttttcgccag gctcgccagc agcgcgttca gcacctgaat atgtttctcc
     8461 tgtttgagaa taaccgcgtc atcttccagc agcagatagt tttgccagcc gtgctgttgc
     8521 gccagatgca gcgcctgcaa gtgtgattgc tggcggcccc gttgaccgtt ttcattttca
     8581 ctggcggcaa ggcgggtgat cttctcaggc ggaatatgca gcaatgtcag ttcttcacgg
     8641 atctgctgta agcgatccgg gcgtttatcc agattcagca gaacgatctg atcgatcgcc
     8701 tgatgctgga tcgcctgata atgagtggcg ttatgcagac ccagataagc gttgcgtagc
     8761 agcggatcac tgacgatatc cgtaagcgcc gcgtttacag gcgtgctggc cccgaggata
     8821 aagtctttat caaaggtgtt gtggctgtgc gagatacaca gaatcgtgcg ctcgccgggc
     8881 agttgcagcg ggttaacgct gaaattgttg gtgaaacttt tctcctcgcc taaattacaa
     8941 tcgtcgtcgt aacggtgctt tttcaggtag ttacggtgat agcaaaaggt gccattgagg
     9001 atgttatgtg ggccaaagct gcggcttttg aaaatgcggt tgatgtggct gtaccagatg
     9061 gggatctggt cggaaccgga gatgagcgcc cggtgttttt gcatcatggc gatggagtaa
     9121 gagattttat ctggcgggta gtagtcgtca tcgtccatgc agaagatgta ttcgccgcgc
     9181 gccagttcgt tgagcatatt gcgttttttg cccagcggta gtttttccgg gtgatggatg
     9241 tagcggatgt tgaaggattc cggcgtgcca ttggtcaggc ggtcgatgat gtgttgatgg
     9301 ctttgcgggg agtcgtcgag gatgatgagt tcgcggcggt ctgccgggta gtcctgatag
     9361 cggtacatat aaagcaggta cggcaggaat gctccgcggt tccaggttgg cgtgacgacg
     9421 ctgacaaacg gttttacgcg cgcggtggcg agtgttttga tgagggtttg catgatagtt
     9481 tacgaagtcg ataaggccgg gaaaaccggc cagaaagtgt tagcaagaca gcagtgaaaa
     9541 cgcataaagc aggggatctg cggaacattc agcctgaatt tgttggcgaa tcagcacggc
     9601 attttcactg gctcgccagc gttcaaacat ggcgaagagg gcgaaaatat cctcctgtcg
     9661 cagcgaagaa ccatccagcg tccataaact gattaacgtg cggaggagat aaaaatcgca
     9721 caccagcggg caataatctg cgccgtctgc ctgtccaatc acctcatggt aaacgcggta
     9781 aatcagccag ttacgcatca tccacggcgc ggtggcaaaa tgctgttgcc agatggtttg
     9841 ccactgtgcc tgaagctgct gttgcaacag ggcttcgctg ttggcgtcat gtagcgtcag
     9901 caaggtaacc tgcaacggca gcgaggcctt gcgtgggctt tccggtagaa tcgaccagtc
     9961 aaacgtaaaa ctcccgagca gcgtcatcaa ctggcgcgcc agctgtggca cgggcgggag
    10021 ctgggcgaac tgtgcctgta gcgtgccttt ttgtgcatga tcggccagcg cctgtggcag
    10081 cgtcgtcaag cgggcaagaa tatctccgtc tctgcccggc attttactgg cgcgggagag
    10141 cacaattccg acggcataca gggaaagctc cggggttaaa tcggggagcg taagggtact
    10201 caaaatcacc tggtttacta ttgcatcgcg cgcggaaagc tctgcggcat tttcttccac
    10261 cgggcggtag gtgagctgta aatcaaaggc gcaggcatcg ttgagcaccg cttcgcagct
    10321 ttctggctct gtcagcatca ggctcaggcg aatctcgttt ttccacacct tgcggacgta
    10381 taaaggcgaa tcggggtggc gtgccataaa attgcgcaca tattccggtt cgtaacattc
    10441 aataaaactc ataggtagtt tccagtcttt attaccttgc aggatgtgca ataagcgtgc
    10501 cgggaatttt tttaatcaaa aacaaaggat tgagaatcag gcggaaaatc cgtttccgcg
    10561 atgggggaag gaattgtcgt tatccctgat agagaaaatc cgcttcgctg cctaaaaaag
    10621 cctgaataaa ttcatactgg cgaattaaca gctcgccgtt tttatcgtta taggctttgc
    10681 agcgttctgc tttttgctca aggcgttgcc acaactgttg tgctgccgcc ttttgtaccg
    10741 ccggaagcca ggcaaaaacc tgcgccagac cgtcacgatt tacgctaaca ccgagcattt
    10801 tcagcaggct atgccgctga tagctatgcc catgcaactg ctcatagtgc tgcgttatct
    10861 cgtcgtttac cgccagcaga tcctcgctgg cgcggcgaat catgcagatc cgctgacgct
    10921 ggagaagttc acgcagggaa tcgtacagtg cgtcgtcgtc acgaattccc tgcaacaacg
    10981 ccttcacctg ttgtaaccgg atactcacgt catcggcctc ttagttctgg aaaaaactca
    11041 gcatgtcgct ggctaactgt tcggtgtcca cttgcaggct gccggaggct agcatcgcct
    11101 gcatttgtgc cactttatcg ctgtcgatat cgctctcttg ctggtcgttc agcgtttgct
    11161 gagcagactg caatccggcc tgggtaatgt cgtcagcgct cagcgtcgtg gcggtggtcg
    11221 ccgtgctttt gcgcggctga ctggagcctg ccattgtggc tgtcgggtga ttgcctggca
    11281 tagtcggggt gattttcatc gcaacctcgt ctcggtattt gggtgggtta ctccggtaaa
    11341 gcggttaaca ggcggcaaaa ctttaatcgc cagcgcaaat tactccgcgc ttaccgttgt
    11401 caccacgccg ctttccgcca ccatcgcgct gatgacccgc tggctgctgg cgttacgcac
    11461 tttcaccatt tcccctttgc gaccattttt cagcgctacg ccgggcattg aggcggtgat
    11521 ccccgcgctc tggtaaacaa tcatcaccgg ttgatcgcgc tcgaccagca ccggtgatat
    11581 cagttcttct ttggtgattg gcctgcccgg ttgcagcgcg tgtttactgc tgaaaccaat
    11641 tgcgtcattg ggttccatca acacctcccg gcgctgggca ctgacgttgt agggcctcag
    11701 ttccacatca ttcgcggtga gcggcgtatc gcgggcaacc agtgacttcg ccattactac
    11761 tggcaccagt acatccgggc gcaccgctac gttcatctgc cagctttgtg ggcaactgac
    11821 ggtgaagttc atccggttga gggcgatgtc cccaggcgaa gtcagcgtaa cgccgggtgt
    11881 tttagtacag ggcgcggcgg tcgcaatctg cgagggaatg tagaccttaa aggtgtagcg
    11941 atagtcatgc cagcgccgct gttgcgccag cgattcaatt ttttggctgg cggcattcag
    12001 tacctgggcg ttgacttgtt cccgcgcgct gtgctgcacg ggatggacgg cggcgtagcc
    12061 cggtaaggcc agcgccagga ggaaataaaa cttccgcctg caaaattgaa aactcataaa
    12121 ccctttctct tcagttactt aaaaaatggc acggcgattg cttagcgtat gcggattgaa
    12181 gcaggagtga cgcaggaaat ggggatcagt tttcagcagg cattgggagt acatccgcag
    12241 gcagtgaagt tacgtcttga gaggaccgag ctactgactg cgaatctggc gaacgtcgat
    12301 acgccaaatt tcaaggctaa agatattgat tttgccaggg agatgcaacg ggcaaataac
    12361 gcggcggtgg atgtccagta ccgtgtgccg atgcagccgt cggaagatgg caacaccgtg
    12421 gaactgaaca gcgaacaggc gcggttttca caaaatagta tggattatca aagcagtctg
    12481 acctttctga atctgcaaat cagcggcatc agagaggcca ttgaagggaa ataagcatgt
    12541 catttactga tatctatcag atttccggct cggcgatgac ggcgcaaacg ctacgtctga
    12601 ataccgttgc cagtaacctg gcgaatgcca atgcgcccgc cagcagcgag gcgcaggcat
    12661 ataaagcgcg cagcccggtg tttgcggcgg tgtatcacca cagtttgctg gcgggtacac
    12721 atcgtcatgc cattgacggt gccagtgtgc aggtgcagga cgtgctgcaa accggcgggg
    12781 ctgtgaaacg ctatgaacca cattcgccgc tcgccgatgc taacggggat gtctggtatc
    12841 ccgacgtcaa cgtggtggaa cagatggcgg atatgatgtc ggcttcgcgc gatttcgaaa
    12901 ccaatgtcga tgtgctcaac aacgtgaaaa gtatgcagca aagtttgctg aaactgggag
    12961 aagcctgatg aataccctgg cgctgaatgc gcaatcaccg cagccgaccg ccacggcgga
    13021 aagcaacaac gtggcggcca gcgacacgac aagcagcagc gatagctcgc ccgttgatac
    13081 tttcctgacg ctgtttgtcg ctgagatcca aaaccaggac ccgaccgacc cgaccgacgc
    13141 caccgaatat atcgaccagc tttcgtcaat ggcgcaggtg gcgatgatgg aagagatgag
    13201 cgtgcaggcc aacaccaacg cggtgctgat gagcaacatt caggtgatgg cgctcggcaa
    13261 tctggtaggc gacgacatta tggtgcagac caccgccttg caggtgagtg accagaccat
    13321 taacggtcgc gccacgcttg acgacgcctg taccaccgcc gatctccact ttaccgatgc
    13381 cgcaggcgat gactatacgg tgtcgctgat tccggaaggc agttcgtccg ttgggccagg
    13441 ccaggtcgat ttctcgatta atccggcgga ttacggtatc ccgccgggcg attacgacgt
    13501 ctcggtagtc accaacaccg gggaagaaga ggtgccgatt gaagtctccg gcgaagtgga
    13561 agatgtacgc atcccgctcg acggcagctc cccggtactt aacgttgccg gtgtaggtga
    13621 agtgccgttc accatgatta gccagtttgg cgtacccgat gccgacagtg atgcggcgtg
    13681 atgtctggtg ccaaattgcc tgatgcgctt cgcttatcag gcctacatga aactgcaatg
    13741 tattgaatct gcatggtttt gtaggccgga taaggcgttc acgccgcatc cggcaaaaca
    13801 acgggcacgc tgccaaaatc tgaattaaca ggaagaaaca atgagttatg aaattgccgc
    13861 aacggggctg aatgccgtta acgaacagct ggacgggatc agtaacaaca tcgccaacgc
    13921 cggaacggta ggctataagt cgatgaccac ccagttttca gccatgtatg ccggaagtca
    13981 ggcgatgggc gtcagcgtgg caggcaccgc gcaaagtatt tcgcgcggcg gttcgctggt
    14041 atccaccggt aacgcgctgg atctggcgat taacgacgac ggtttttttg ttacctgcga
    14101 cagcgcgggc aacatttctt atacccgcgc cggatcgttt gaaaccgaca aaaacggcta
    14161 tatcgtcaac gcctcgggcg cttatttgca gggctatccg gtcgatgaca cgggcaccct
    14221 gcaaaccggt acagtcaccg atatccagat caaaaccggt aatattccgg cgcaggcgag
    14281 cagcagcctg acctttaccg ccaacttcga tgccagcgat gcggctatcg accgcactac
    14341 cgtaccgttc gacgcgacca acagcagctc gtacaccgac agctacacca ccacggtata
    14401 cgactcatta ggcaacgaac attcggtatg ccagtacttc accaaaacca gcgataacac
    14461 ctgggaagtg cagtacacct tcgacggtca gcagcagacc ggcgttccgg cgacgacgtt
    14521 aaccttcgac ccgaacaccg ggaaactgac ctcgccaaac acgccgcaga ccattgagtt
    14581 tcagaccgac gccgccgcac ccatcgattt aaccgtcgat tactcgacct gtacgcaata
    14641 cggctctgaa ttttcggtca ccaccaacgc cgccgacggc tacgcctctg ccactcagaa
    14701 cggtgtgcag gttgatgacg atggcaaagt ttacgctacc tacagcaacg gcgagcgcat
    14761 gttgcagggg caggtggtgc tggcgacgtt cccgaatgaa aacggcctcg aagcggtgag
    14821 cggcaccgtg tgggtacaaa ccggcgaatc cggcaccccg ttgattggcg ttcccggttc
    14881 cggcacctgc ggcacgctgt cgtcgggcgt gctcgaaagc tctaacgtcg atatcaccag
    14941 cgagttagtc aacctgatga ccgcccagcg taactatcag gccaacacca aagttatcgc
    15001 caccagcacg cagctcgatg acgcgctgtt ccaggcaatg taatggatcg tttgatttat
    15061 accgccctga gcggtgcttc tcagaccctg tacgagcagc agatcagcgc caacaacctg
    15121 gcaaacgtca acaccaacgg ttttcgcgcc gatatggcaa tggcgactaa cgacaaggtg
    15181 aaaggcggcg gcttcgacac gcgctatatg gcgcaggaag gggccagcgg cgtgaacgac
    15241 agcaccggtg tggcggagaa aaccgaacgc ccactggacg tggcgattca gggttcaggt
    15301 tacattgctg tgcaggataa aaacggcaac gaagtgtata cccgcaacgg caacattcag
    15361 caggatgacc aggggcaact gactatcgac ggcaatctgg tgctgggaga taacgggccg
    15421 atcattctgc cgcccaacgc cattgcttcg tttggcagcg acggcacgct ctccgtgacg
    15481 cccgatgacg gtgacgtgac cgcgacgatg gatatcgacc gcctgaagct ggtggatatc
    15541 ccggtcgcga acctggcgaa aaatcctgaa gggatgctgg tgactgccga cggcgttccg
    15601 gcccagcgcg atgaaaacat caaagtcagc ggcggttttc tcgagggcag caacgtctcg
    15661 gcggtgagtg agatgatgtc gtccatcgcc atgaaccgcc agttcgaagc gcagatcaag
    15721 atgatgaaaa ccgccgaaga tatcagcgac gcgggcaacc gcttattgcg cggctcgtaa
    15781 ccgcacatcg taggtcggat aaggcgttca cgccgcatcc ggcaaccgcg gcccaaaaat
    15841 aacacggaat ttaccaccat gaacgcagca ttatggatca gcaaaaccgg cctctcggcg
    15901 caggatgctg aaatgagcgc gattgccaac aacatcgcca acgttaacac cactggcttt
    15961 aagcgcgacc gggtgatgtt tcaggatctg ttctaccaga cccaggaagc gccgggcgcg
    16021 atgctcgacc agaacaacat catgccgacc gggctgcaat tcggtagcgg cgtgcgcatt
    16081 gtcggtactc agaaaacctt caccgaaggc aacgtcgaaa ccaccgacaa cgcgatgaat
    16141 gtcgccatta tggggcaggg atttttgcag gtgcaaaagg ccaacggtga tatcgcctat
    16201 acccgcgacg gcaacttgca ggtgaacgct gacggcgtac tgaccaactc gcagggcttg
    16261 ccgttgcagc cggaaatcga cgttccggcg ggggcgacca acgtcgcgtt tggtgaggat
    16321 ggcacggtga cggcgatctt acccggcgac agcgacgtga ctgagttagg gcaactgacg
    16381 ctggtgaact tcgccaaccc ggccgggctt tctgccgagg gcgacaacct ctatctggaa
    16441 accgccgcca gcggacagcc gaccgaaggc gtaccgggcg aagatgggct tggcaccttg
    16501 caggataacg cgctggaagg ttcgaacgtg gatatcgtca acgaaatggt ggcgatgatt
    16561 acggtgcaac gcgcgtacga gatgaacgcc aaaatggtgt cagcagcgga cgatatgttg
    16621 cagtacatca gccagacgct gtaaggccga aatgtcggat gcgacgctga cgcgtcttat
    16681 ccgacctaca agagcacata actcactgta tttaaacagg cagaacgtga aaaattatct
    16741 ctggctggtg gcgctgcttc cactccttag cgggtgtgaa tcgcaggcca ttctggtcaa
    16801 aaaagacgac gcatactttg cgccaccaac caccgaagcg ccgcctccgg cagacgggcg
    16861 tgccgggggc gtgtttgaaa ccggctacaa ctggtcgcta actgccgacc ggcgggctta
    16921 ccgcgtgggc gatatcctga cggtgatcct cgaagaatcc acccagtcga gcaaacaggc
    16981 gaaaaccaac ttcggcaaaa gcaacaccgt cgatatcggc gcgccgacca tttttggaca
    17041 caccaaagat aagctttccg gctccattga tgccaaccgc gactttgacg gcagcgccac
    17101 ttcccagcag cagaacagcc tgcgcgggga gatcaccgtt tccgtccatg cggtgcagcc
    17161 caacggtatc ctggaaattc gcggcgaaaa atggctgacc ctcaaccagg gcgatgagta
    17221 catccgttta agcggcctgg tgcgcgccga tgatatccag aacgacaact cggtttcctc
    17281 gcagcggatc gccgatgccc gcatctccta tgccggacgc ggcgcgttaa gcgacgccaa
    17341 cgccgcaggc tggttgacgc ggctgtttaa ccatcctctg tttccgattt aatgccggag
    17401 agtgcaatgc aaaactggat aaaggccgca gcgatcgctg taagtctgac gctgcccggc
    17461 gtggcgctgg cgcaatcgct ggagtcgttg gtcaacgtac agggcgtgcg tgaaaaccaa
    17521 ctggtcggct acagcctggt ggtcggcctc gacggtactg gtgataaaaa ccaggtcaaa
    17581 ttcaccaacc agaccatcac caatatgctg cgccagtttg gcgtgcaact gccaaataaa
    17641 atcgatccga aggtgaaaaa cgtcgccgcg gtagcggtga gcgccacgct gccgccgatg
    17701 tactcgcgcg ggcagaccat tgatgtcact gtttcgtcga ttggcgatgc caaaagtatt
    17761 cgtggcggca ctttgttgct gacccagttg cacggcgcgg atggcgaagt ttatgcgctg
    17821 gcgcagggca gcgtggtggt gggcgggatg aatgccaccg gggccagcgg ttcgagcgta
    17881 acggttaata cgccaaccgc cgggttaatt cctaacggcg caacggtaga gcgcgagatc
    17941 cccagcgatt ttcagatggg cgacactatc accctcaacc tgaaacgtcc gtcgtttaaa
    18001 gatgccaaca atattgcggc ggcgattaac gcctcgtttg gcggcattgc tactgcgcag
    18061 agctctacca acgtcagcgt gcgcgcgccg accagccccg gcgcgcgggt ggcatttatg
    18121 tcgcaactgg atgatgtaca ggtgcaggca gagaaaatcc gcgcccgcgt ggtgtttaac
    18181 tcgcgtaccg gcacggtggt gatgggcgat ggcgttgcgc tgcacgcggc ggcggtgtcg
    18241 cacggtagcc tgaccgtctc gattaacgaa accagcaacg tcagccagcc gaatgccttt
    18301 gcgggcgggc gcacggcggt aacgccgcag agtaatatcg cggtgaatca tgcccgtccg
    18361 ggcgtggtga gtttgccgga gtcgagcagc ctgaaaacgc tggtcaatgc gttgaacagt
    18421 ttaggcgcga cgccggacga cattatgtcg attttacagg cgctgcatga ggcgggcgcg
    18481 ctggatgccg acctggaggt gatctgatga aagtgaacgc cagcggcggg attgatggca
    18541 gcgatgcgtt aatggggccg aaggttcaga ccaatgatat taagcaggcc gcggagcagt
    18601 ttgaggcgat ttttttacgc aacatgctga aagagatgcg taaaaccaac gagttgtttg
    18661 attcgaaaga taacccgttt aacagcgatt cggtgcgcat gatgcagggg ttttatgacg
    18721 atgagttgtg taacaccctg gcgcagcagc acgggattgg gattgcggcg atgattgtga
    18781 agcagttgtc gccgaagcat aaatgaggct ccgacgttcg gtgcctgatg cgacgctgtc
    18841 tcgtcttatc aggcctacga gtccgcacct gtttttatgc gtgtgtccgt ttgtaggccg
    18901 gataagg
//
LOCUS       DADSZD010000083        18864 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.083, whole genome shotgun sequence.
ACCESSION   DADSZD010000083 DADSZD010000000
VERSION     DADSZD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.083"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            275..1318
                     /gene="nadA"
                     /locus_tag="J1Z28_002641"
     CDS             275..1318
                     /gene="nadA"
                     /locus_tag="J1Z28_002641"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBA7717464.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLLERALVP
                     LNRMLDFAATLRG"
     gene            1356..2075
                     /locus_tag="J1Z28_002642"
     CDS             1356..2075
                     /locus_tag="J1Z28_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBA7717465.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(2072..3013)
                     /gene="zitB"
                     /locus_tag="J1Z28_002643"
     CDS             complement(2072..3013)
                     /gene="zitB"
                     /locus_tag="J1Z28_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBA7717466.1"
                     /translation="MAHSHSHTSSRLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(3127..3507)
                     /locus_tag="J1Z28_002644"
     CDS             complement(3127..3507)
                     /locus_tag="J1Z28_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717467.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            3823..4875
                     /gene="aroG"
                     /locus_tag="J1Z28_002645"
     CDS             3823..4875
                     /gene="aroG"
                     /locus_tag="J1Z28_002645"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBA7717468.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAARLLTLREELQDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCTDVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVKARRG"
     gene            complement(5041..5793)
                     /gene="gpmA"
                     /locus_tag="J1Z28_002646"
     CDS             complement(5041..5793)
                     /gene="gpmA"
                     /locus_tag="J1Z28_002646"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBA7717469.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(5996..7036)
                     /gene="galM"
                     /locus_tag="J1Z28_002647"
     CDS             complement(5996..7036)
                     /gene="galM"
                     /locus_tag="J1Z28_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBA7717470.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKIYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(7030..8178)
                     /gene="galK"
                     /locus_tag="J1Z28_002648"
     CDS             complement(7030..8178)
                     /gene="galK"
                     /locus_tag="J1Z28_002648"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBA7717471.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALFPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(8182..9228)
                     /gene="galT"
                     /locus_tag="J1Z28_002649"
     CDS             complement(8182..9228)
                     /gene="galT"
                     /locus_tag="J1Z28_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBA7717472.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGETYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(9238..10254)
                     /gene="galE"
                     /locus_tag="J1Z28_002650"
     CDS             complement(9238..10254)
                     /gene="galE"
                     /locus_tag="J1Z28_002650"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBA7717473.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(10517..11989)
                     /gene="modF"
                     /locus_tag="J1Z28_002651"
     CDS             complement(10517..11989)
                     /gene="modF"
                     /locus_tag="J1Z28_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBA7717474.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLVGVQMPEPDEPSVRHALPAHEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GKTQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            complement(12057..12845)
                     /gene="modE"
                     /locus_tag="J1Z28_002652"
     CDS             complement(12057..12845)
                     /gene="modE"
                     /locus_tag="J1Z28_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBA7717475.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDDAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            12974..13123
                     /locus_tag="J1Z28_002653"
     CDS             12974..13123
                     /locus_tag="J1Z28_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump-associated protein, AcrZ
                     family"
                     /protein_id="HBA7717476.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            13286..14059
                     /gene="modA"
                     /locus_tag="J1Z28_002654"
     CDS             13286..14059
                     /gene="modA"
                     /locus_tag="J1Z28_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7717477.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            14059..14748
                     /gene="modB"
                     /locus_tag="J1Z28_002655"
     CDS             14059..14748
                     /gene="modB"
                     /locus_tag="J1Z28_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBA7717478.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            14751..15809
                     /gene="modC"
                     /locus_tag="J1Z28_002656"
     CDS             14751..15809
                     /gene="modC"
                     /locus_tag="J1Z28_002656"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBA7717479.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDADKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLEEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(15810..16628)
                     /locus_tag="J1Z28_002657"
     CDS             complement(15810..16628)
                     /locus_tag="J1Z28_002657"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
                     /protein_id="HBA7717480.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSYLI"
     gene            16783..17778
                     /gene="pgl"
                     /locus_tag="J1Z28_002658"
     CDS             16783..17778
                     /gene="pgl"
                     /locus_tag="J1Z28_002658"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBA7717481.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(17819..18772)
                     /locus_tag="J1Z28_002659"
     CDS             complement(17819..18772)
                     /locus_tag="J1Z28_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7717482.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT         4476 a         4697 c         4940 g         4751 t
ORIGIN      
        1 caaaaaagcg ccctaaaggc gcttttttgc tattcaggca tctctcaatt tcaattcttg
       61 accctgatga catactccgc gcttgctgcg taaacaactc tacgtcggag gaataagacg
      121 caaagtgtgg cattcggcat ctcatacgta attcgagtga cttttcccca ccattcgact
      181 atcttgttta gcatataaaa caaattacgc cgataatagc gaatatcacg cttaagtcgg
      241 ttttaacgtt aagcctgtaa aacgagatgg taagatgagc gtgatgtttg atccagacac
      301 ggcgatttat cctttccccc cgaagccgac gccgttaagc attgatgaaa aagcgtatta
      361 ccgcgagaag ataaaacgtc tactaaaaga acgtaatgcg gtgatggttg cccactacta
      421 taccgatccc gaaattcaac aactggcaga agaaaccggt ggctgtattt ctgattctct
      481 ggaaatggcg cgcttcggtg caaagcatcc cgcttctacg ttgttagttg ctggggtgag
      541 atttatggga gaaaccgcca aaattctcag tccggaaaaa acaattctga tgccgacact
      601 tcaggctgaa tgttcactgg atctcggctg ccctgttgaa gaatttaacg cattttgcga
      661 tgcccatccc gatcgtactg tcgtcgtcta cgccaacact tctgctgcgg taaaagcgcg
      721 cgcagattgg gtggtaactt caagcattgc cgtcgaactt attgatcatc tggatagttt
      781 gggtgaaaaa atcatctggg cacctgacaa acatctgggg cgttacgtgc aaaaacagac
      841 gggcgcagac attttatgct ggcagggtgc ctgcattgtg catgatgagt ttaagactca
      901 ggcgttaacc cgcttgcaag aagaataccc ggatgctgcc atactggtgc atccagaatc
      961 accgcaagct attgtagaga tggcggatgc ggtcggttcc accagtcaac tgatcgctgc
     1021 tgcgaaaaca ttgccacatc agcggcttat cgtggcaacc gatcggggca ttttctacaa
     1081 aatgcagcag gcggtgccag ataaagagtt actggaagca ccaactgcgg gtgagggcgc
     1141 aacctgtcgc agctgcgcgc attgcccgtg gatggccatg aatggccttc aggccatcgc
     1201 agaggcatta gaactggaag gaagcaatca cgaggtttat gttgatgaaa ggctgctaga
     1261 gagggcgctg gtgccgctca atcgtatgct ggattttgcg gctacactac gtggataatt
     1321 gataataagg cgtcacatta cgctttgggg gaacgatgga tttttttagt gtgcagaata
     1381 tcctggtaca tataccaata ggggcaggcg gttatgatct ctcatggatc gaagcggtag
     1441 gcacgatcgc cgggttgctg tgtattggcc ttgccagtct ggagaagatc agtaactact
     1501 tctttggcct gatcaacgtc actttgtttg gcattatttt ctttcagatt cagctgtatg
     1561 ccagcctgct attacaggtg tttttctttg ccgcgaatat ttacggctgg tatgcgtggt
     1621 cgcgacaaac cagtcagaac gaggcggagt tgaaaattcg ctggttgcca ttgccgaagg
     1681 cgctaagctg gttggcggtt tgcgttgttt cgattggtct gatgacggta tttatcaatc
     1741 cggtgtttgc atttttgacc cgcgtggcag tcatgatcat gcaagcatta ggattacagg
     1801 ttgcgatgcc tgaactacag ccggacgctt tcccgttctg ggattcatgc atgatggtgt
     1861 tatctatcgt ggcaatgatt ctgatgacgc gtaagtatgt ggaaaactgg ctgttgtggg
     1921 tgattattaa cgtgattagc gtcgttattt ttgcacttca gggcgtttac gccatgtctc
     1981 tggagtacat catcctgacc tttattgccc tcaacggcag ccggatgtgg atcaacagcg
     2041 cacgcgaaag aggttcacgc gcgctgtccc attaatggtg atgatgtgaa tggccagaca
     2101 ccccttcatt caggtggcag tctggcccat gacaaggttg atattccatc tgaatagtgg
     2161 cgtgctcaat ctgatagtga tccatcaggt agtgttggat ctgatctaac aaggcatcgt
     2221 gatcgtgtgg cgggatcacc tgcacatgca gcgtcatcac cggcttctcg cctaccatcc
     2281 atacatgtac atggtgcaca ttgcggactt ccgggatttc ccggcacata cgacgcttca
     2341 gttcggcgat atccagcgat accggtgcac cttcaagtaa ttcattcacg ctatctttca
     2401 acagtcgcca cgcgctacgc agaaccagaa gcgacaccag tattgagaga atggggtcag
     2461 caggtgtcca gccggtccag ataataatca acgcggcaat aatagcccca accgaaccca
     2521 gcaggtcccc gagcacatgc agtgccgctg ctcgcacgtt gaggtttttc tcttcgctgc
     2581 cgtgatgaag taaccagaaa gaaagtatat ttgccagcag cccagccacg gcaattgcca
     2641 tcatcatgcc gccctcgacc ggacgcggag tgcggaagcg ttctatcgcc tcccagacaa
     2701 tcaaaatagt aatcaccacc agggcgatgg cattcacaaa cgccgcgagc gtggtcaatc
     2761 ttagccaacc gaaagtgtgg cgaatggtgg gaggacgacg ggaaaattgc actgcgagta
     2821 gggcgaaaag cagggcggca gtatcggtca acatatgacc cgcatcggcc agcaatgcca
     2881 gagaaccgga aagaaaacca ccaatgactt ctacgagcat aaacccggca gtcacgccga
     2941 aagcatacaa caagcgacga gcattattat cttctggcag gcgtgaggaa gtgtgtgagt
     3001 gtgagtgcgc catgacgagt catcccttaa ttattctcac ttaactttat gacatcatac
     3061 tgcttttaga agggaaaaat taaaagggag agactccgct ctcccattat tggctatttc
     3121 gcagggttac tgcgtggtac cgtcggtttt ggtatcgaca tcattgttga tgccatcacc
     3181 ggtttggact tttttattga tatccgggca gcgaccatct ttgcacatgg tgttcttgtg
     3241 ctcttcatct ttggtcatgc cgtcattgtt cattgaagaa ccatccggat gcagcattgt
     3301 gccgccagaa ccggtattta ccccgttatt gtcgacgtta tttggcgcga cattttcacg
     3361 ggcatcaggg gctacctggc ccgcatcagc tgcggcgttt gcctggccgt tattgctttg
     3421 cgctccgcta tcggcagcca gtgcggcacc gctggcaagg cttagagtgg cagtcaaaaa
     3481 tagtgtggcc agttttgtca ttttcatagg atgctcctgt tatggtcgtt atgtcggata
     3541 acctcttcca acagtgcatt tgcaggtgaa tataaggcaa tggtttaaga tttcagccag
     3601 gcaaagatac ccagcagaga atcttgaaat aattaacaaa caaaggagtt acagttagaa
     3661 attgtaggag agatctcgtt tttcgcgaca atctggcgtt tttcttgcta attccaggat
     3721 taatccgttc atagtgtaaa accccgttta cacattctga cggaagatat agattggaag
     3781 tattgcattc actaagataa gtatggcaac actggaacag acatgaatta tcagaacgac
     3841 gatttacgca tcaaagaaat caaagagtta cttcctcctg tcgcattgct ggaaaaattc
     3901 cccgctactg aaaatgccgc gaatacggtc gcccatgccc gaaaagcgat ccataaaatc
     3961 ctgaaaggta atgatgatcg cctgttggtg gtgattggtc cgtgctcaat tcatgatcct
     4021 gtcgcggcta aagagtatgc cgctcgcttg ctgacgctgc gtgaagagct gcaagatgag
     4081 ctggaaatcg tgatgcgcgt ctattttgaa aagccgcgta ctacggtggg ctggaaaggg
     4141 ctgattaacg atccgcatat ggataacagc ttccagatca acgacggtct gcgtattgcc
     4201 cgcaaattgc tgctcgatat taacgacagc ggtctgccag cggcgggtga gtttctcgat
     4261 atgatcaccc cacaatatct cgctgacctg atgagctggg gcgcaattgg tgcacgtacc
     4321 accgaatcgc aggtgcaccg cgaactggcg tctggtcttt cttgcccggt aggcttcaaa
     4381 aatggcactg atggtacgat taaagtggct atcgatgcca ttaatgccgc cggtgcgccg
     4441 cactgcttcc tgtccgtaac gaaatggggg cattcggcga ttgtgaatac cagcggtaac
     4501 ggcgattgcc atatcattct gcgcggcggt aaagagccta actacagcgc gaagcacgtt
     4561 gctgaagtga aagaagggct gaacaaagca ggcctgccag cgcaggtgat gatcgatttc
     4621 agccatgcta actcgtcaaa acaattcaaa aagcagatgg atgtttgtac tgacgtttgc
     4681 cagcagattg ccggtggcga aaaggccatt attggcgtga tggtggaaag ccatctggtg
     4741 gaaggcaatc agagcctcga gagcggggaa ccgctggcct acggtaagag catcaccgat
     4801 gcctgcattg gctgggatga taccgatgct ctgttacgtc aactggcgaa tgcagtaaaa
     4861 gcgcgtcgcg ggtaaagttt aattgtcgga tgcgccgtca gagtggcgta tccgattaat
     4921 caccggaggc ctgataaaac gcgcagcgtc gcatcaggca atgtgctcca ttgttagcaa
     4981 cgacaaacaa aaaagccgac tcacttgcgg tcggctttct catttttaaa cgaatgacgt
     5041 ttacttcgct ttaccctggt tagcaaccgc cgctgctttc gccgcgatct cgtcagcatt
     5101 acccagataa tagcgtttca gcggtttgaa gttctcgtca aactcataaa ccagcggcac
     5161 gccggtcggg atattcagct caagaatctc ttcttcgctc atgttatcga gatatttcac
     5221 cagcgcacgt aacgagttac cgtgtgcggc gatgatgacg cgctcaccgc tcttcatacg
     5281 tggcagaatg gtttcattcc agtaagggat cacgcggtca atggtcagcg ccaggctttc
     5341 cgtcagcggc agctctttct cgctcagttt cgcgtaacgc ggatcgtgcc ccggataacg
     5401 ctcatcatct ttagtcagtt ccggcggagt caccgcaaaa ccacgacgcc actgtttcac
     5461 ctgctcgtcg ccatactttt cagcggtttc cgctttgttc agaccctgca acgcaccgta
     5521 gtgacgttcg ttcagtttcc aggatttctc aacgggcagc catgcctgat ccagttcgtc
     5581 caacacattc cacagggtat gaatagcgcg tttcagcaca gaggtgtaag caaagtcaaa
     5641 gctgtagcct tcctctttca gcagcttacc tgctgctttt gcttcgctta cgcctttctc
     5701 agacagatcc acgtcgtacc aaccggtgaa acggttttct ttgttccact gactttcgcc
     5761 atgacgaacc agaaccagct tagttacagc catatactta ctcctcaaat catcttttaa
     5821 tgataataat tctcattata ttgccgtgac gaagcaacag caatgcttac gcataaccat
     5881 agcgaaaata gtgtcgcagt gtaaggttgt tgtgaatatt gagttgtaaa tatgtcggtg
     5941 tttgctggtg atttgaacaa tatgaagata aagccctcat gacgagggct taacattact
     6001 gagcaataaa ctgatattcc gtcaggctgg aatactcttc gccaggacgc aggaagcagt
     6061 ccggttgcgg ccattcaggg tggttcgggc tgtccggcag aaattcgctt tccagagcca
     6121 gcccttgcca gtcggcgtaa ggttcggttc cccgcgacgg tgtgccgccg aggaagttgc
     6181 cggagtagaa ttgcagagcc ggagcggtgg tgtagatctt cagctgcaat ttttcatctg
     6241 ctgaccagac atgcgccgcc actttcttgc catcgccttt ggcctgtaac aagaacgcgt
     6301 gatcgtaacc tttcactttg cgctgatcgt cgtcggcaag aaactcactg gcgatgattt
     6361 tggcgttgcg gaaatcaaaa gaggttccgg cgacagattt caggccgtcg tgcggaatgc
     6421 cgccttcatc aaccggcaga tattcttccg ccagaatctg caacttgtga ttgcgcacgt
     6481 cagattgctc gccgtcaaga ttgaaataga cgtgattagt catattcacc gggcaaggtt
     6541 tatcaactgt ggcgcgataa gtaatggaga tacggttatc gtcggtcaga cgatattgca
     6601 ccgtcgcgcc gagattaccc gggaaaccct gatcgccatc atctgaactc agggcaaaca
     6661 gcacctgacg atcgttctgg ttcacaatct gccagcgacg tttgtcgaac ccttccgggc
     6721 cgccatgcaa ttggttgacg ccctgacttg gtgaaagcgt cacggtttca ccgtcaaagg
     6781 tataacggct attggcgatg cggttggcat aacgaccaat agaggccccc agaaatgcgg
     6841 cctgatcctg atagcattcc gggctggcgc agccaagcag cgcctcgcgg acgctgccat
     6901 cggaaagcgg aatacgggcg gaaagtaaag tcgcacccca gtccatcagc gtgactacca
     6961 tccctgcgtt gttacgcaaa gttaacagtc ggtacggctg accatcgggt gccagtgcgg
     7021 gagtttcgtt cagcactgtc ctgctccttg tgatggttta caaacgtaaa aagtctcttt
     7081 aatacctgtt tttgcttcat attgttcagc gacagcttgc tgtacggcag gcaccagctc
     7141 ttccgggaac aacgcgacga tacagccacc aaatccgccg ccggtcatgc gtacgccacc
     7201 tttgtcgcca atcacagctt tgacgatttc taccagagtg tcaatttgcg gtacggtgat
     7261 ttcgaaatca tcgcgcatag aggcatgaga ctccgccatc aactcgccca tacgtttcag
     7321 gtcgccttgc tccagtgcgc tggcagcttc aacggtgcgg gcgttttcag tcaggatatg
     7381 acgcacgcgt ttcgccacga tcgggtccag ttcatgcgca acagcgttga actcttcaat
     7441 ggtgacatcg cgcagggctg gctgctggaa gaaacgcgca ccggtttcgc actgttcacg
     7501 acgggtgttg tattcgctgc caaccagggt acgtttgaag ttactgttga taatgacgac
     7561 agccacacct ttgggcatgg aaactgcttt ggtccccagt gagcggcagt caatcagcaa
     7621 ggcatgatct ttcttgccga gtgcggaaat tagctgatcc atgatcccgc agttacagcc
     7681 aacaaactgg ttttctgctt cctgaccgtt aagcgcgatt tgtgcgccgt ccagcggcag
     7741 atgataaagc tgctgcaata cggttccgac cgcgacttcc agtgaagcgg aagaacttaa
     7801 ccctgcaccc tgcggcacat tgccgctgat caccatgtcc acaccgccga agctgttgtt
     7861 acgcagttgc agatgtttca ccacgccacg aacgtagttc gcccattgat agttttcatg
     7921 cgcaacaatg ggcgcatcga gggaaaactc gtcgagctga ttttcataat cggctgccat
     7981 tacgcgaact ttacggtcat cgcgtggcgc acagctgatc acggtttgat aatcaatcgc
     8041 gcagggcaga acgaaaccgt cgttgtagtc ggtgtgttca ccaatcaaat tcacgcggcc
     8101 aggcgcctga atggtgtgag tggcagggta gccaaatgcg ttggcaaaca gagattgtgt
     8161 tttttctttc agactcattt cttacactcc ggattcgcga aaatggatat cgctgactgc
     8221 gcgcaaacgc tctgctgcct gttctgcggt caggtctcgc tgggtttctg ccagcatttc
     8281 ataaccaacc ataaatttac gtacggtggc ggagcgcaac agaggcggat aaaagtgcgc
     8341 gtgcagctgc cagtgttgat tctcttcgcc attaaatggc gcgccgtgcc agcccataga
     8401 gtaggggaag gagcactgga agaggttgtc ataacgactg gttagctttt tcaacgccag
     8461 tgccaaatcg ctgcgctggg cgtcggtcaa atcggtgatc cgcaaaacgt gggctttggg
     8521 cagcagtagc gtttcgaacg gccaggcagc ccagtaaggt acaacggcta accagtgttc
     8581 ggtttcgaca acggtacggc taccgtctgc cagctcgcgc tgaacataat ccaccagcat
     8641 tggcgatttc tgttcggcga aatattcttt ttgcagacgg tcttcgcgct cagcttcgtt
     8701 aggcaggaag ctatttgccc aaatctgtcc gtgcggatgc gggttagagc agcccatcgc
     8761 cgcgcctttg ttttcaaaga cctgcaccca tgggtacgtt tcccccagct ctgcggtttg
     8821 ctcctgccag gttttgacga tttccgtcaa tgccgcaacg ctcagttctg gcaacgtttt
     8881 actgtgatcc ggtgaaaagc agatcacccg gctggtaccg cgcgcgctct ggcaacgcat
     8941 tagcggatcg ttgctttctg gcgcatctgg cgtgtcagac atcaaggccg caaagtcatt
     9001 agtgaaaacg taagtcccgg tgtaatcggg gtttttatcg cctgtcaccc gcacattacc
     9061 tgcgcagagg aagcaatctg gatcgtgcgc aggtaacacc tgtttggctg gcgtttcctg
     9121 cgccccctgc caggggcgct tagcgcggtg cggtgaaacc agaatccatt gcccggtgag
     9181 cgggttgtag cggcgatgtg gatgatcaac gggattaaat tgcgtcatgg tcgttcctta
     9241 atcgggatat ccctgtggat ggcgtgactg ccagtgccag gtgtcctgcg ccatttcatc
     9301 gagtgtgcgc gttacgcgcc agttcagttc acggtcggct ttgctggcgt ccgcccagta
     9361 ggccggaagg tcgccctcgc gacgcggtgc aaaatgataa ttaaccggtt tgccgcaggc
     9421 tttgctgaag gcattaacca cgtccagcac gctgctgccc acgccagcgc cgaggttgta
     9481 gatgtgtacg cctggcttgt tcgccagttt ttccatcgcc acgacgtgac cgtccgccag
     9541 atccattacg tggatgtaat cgcgcacgcc agtaccgtct tcggtcggat aatcgttacc
     9601 aaaaatcgcc agcgagtcgc gacggcctac agcaacctgg gcgatgtatg gcatcaggtt
     9661 attcggaatg ccttgcggat cttcgcccat atcgcccgac ggatgcgcgc caaccgggtt
     9721 gaagtagcgc agcagggcaa tgctccagtc cggctgggct ttttgcagat cggtgaggat
     9781 ctgttccacc atcagcttgc ttttgccgta agggctttgc ggtgtgccgg tcgggaagct
     9841 ttcaacatat ggaattttgg gttgatcgcc ataaacggtg gcggaggagc taaagataaa
     9901 gtttttgacg ttagcggcgc gcatggcgct aatcaggcgc agggtgccgt tgacattgtt
     9961 gtcgtaatat tccagcggtt tttgtaccga ttcgccaacg gctttcagcc cggcgaagtg
    10021 gatcacggtg tcgatagcgt gatcgtgcag gatctcggtc atcaacgctt cgttacggat
    10081 atcgccttca acaaacgttg gatgtttgcc gcctaaacgc tcgataacag gcagtacgct
    10141 gcgcttactg ttacagaggt tatcaagaat gatgacatca tgaccgtttt gcagtaattg
    10201 cacacaggta tgacttccaa tgtaaccgct accaccggta accagaactc tcataattcg
    10261 ctccattagg cttatggtat gaaataacca tagcataaca aagatgcgaa aagtgtgaca
    10321 tggaataaat tagtggaatc gtttacacag gaatttagcc gttttttatg cgcgattaag
    10381 tgattataaa acagcggctt tatgaatgat tgcgcttttt atctgaaaaa aaagacgcgg
    10441 tttcatgcct ggatgcgtca aaccgttggc cggataacac gtttacgccg cctccggcac
    10501 ggtcagcact accgactcag tttatttttg tcagcgcata gcgataaaag tcaccgtccg
    10561 gcacgaactc aagacgatgg gtaatacagg caggcgcatc ttcagcgtgg tgcgaaacaa
    10621 acaacaattg cgttttacct tcgctaatca gcacatcaac aaaacggcgg ataagctggc
    10681 gattgagcgg atcaagcccc tgtagtggtt catcgagaat aagcaacgtc ggatgtttca
    10741 ctagcgcacg gacgatcagc gccagacgct gctgtcccca ggaaagacta tggaaaggag
    10801 cgtcagccgt gcgtttatca atgccgagaa tatccagcca ctgctgcacc agtttttgct
    10861 ggcgatccga aacggcctga taaatgccaa tcgaatcaaa atagccagaa agaatcacat
    10921 tacgcacagt agtgctgacc cggtaatcca gatgcaaact actgctgacg taaccgatat
    10981 gctttttgat atcccagatg gtttcgccgc tgccgcgacg tcgtccgaaa agcgtcaaat
    11041 cgttgctgta accttgcgga tgatcgccag taatcaggct taataacgtc gattttcccg
    11101 cgccatttgg cccgacaatt tgccagtgtt cgcctggatt cacctgccag ctaaggttat
    11161 taaggatggg gcgttcgtta taagaaacca cgccattgtt cagcacaatg cgcggttcgt
    11221 gggcgggtaa ggcgtgacgt actgaaggtt catccggctc cggcatttgc acacctacaa
    11281 gcagttcgct atgcgccagt tgcgcaacga gtgcttgttg gagcagttct tctttagcgc
    11341 cagtttccgc taacgtgcaa tccgccagca cgccagcaaa ctggacaaat tccgggatct
    11401 catcgaagcg attgagcacc agtaccagag taataccgga ctgatgtaac gaggcgagta
    11461 gctcagccag ctgctgacgt gaggcaacat ccaggccatc aaacggttca tcaaggatca
    11521 acaagtcagg ctccgacatc agcgcctgac acagcagggt ttttcgcgtc tcgccagtgg
    11581 aaaggtattt aaagcgtcgg tcgaggaggg cggtaatacc gaactgctgc gccagttgca
    11641 tgcaacgcgg agtatccttt acttcatcct gaatgatctc agccgtggta cgcccggtgt
    11701 catcttcgcc aggactaagc atatcggtgt tattccgctg ccattcgtcg ctgacgagct
    11761 tttgcaattg ctcgaaggag agacgagtga tgtgggaaaa ctggctttgc cgttcacctt
    11821 tcaaaagcgg aagttccccc gacagcgcgc gggccagcgc cgatttcccg cttccattcg
    11881 aaccgacaaa cgcccaacta tcacccgcgt ttaacgttag ccgaggcaat tgcagcgttt
    11941 ttgtgtcgcc aagacgaaac gtgccttgca aaatttgcaa cgatgacatt ttatatccca
    12001 ttttgtgcag cgattactga cagggatacg tgtttcataa caaattgtca acacgcttag
    12061 cacagcgtgg cgataatcac gctgtcggca ttaaagtagg ccgtgacatt ctgcccttgc
    12121 tgaagagaag tcgcttcatt taccggcact gtggcgcaca gtgtttgccc gtcgggtagc
    12181 gccattaata cttcgcactg ctctgcgccg cgctcaatat gactaataat acctggtaat
    12241 tggttgtcag cgttttgcgc gaccgcatcg tcctgagtaa tacctaccca cggcgctttt
    12301 agcaatatca acacttcttt gccttcatcc agccccagac gcgcgccgct ttgtgcggta
    12361 attgcgactt tcaggcgtgt ttttccatca gccagtaaga catcaacatg ctgttgaacg
    12421 tcatcatgat cgcgggcggt gatggtaccg aaccactggt tacgggcgct ggtttgcagt
    12481 gaaaaacgtg agatcgcggc cagcaggctg ttcagcggca gggcgtcatc gtcacttaac
    12541 acatcaaagg ctttttgctg gatttgcgcc agtaagtcat agagttgaat cagtcgctga
    12601 ccatagcggg tcagtactgc gccgcctcca cctttaccgc ctgttgcgcg ctcaaccaga
    12661 atatgctcac ttaactgatt catctcgtta atggcatccc aggcgctttt atagctgata
    12721 ccggcatctt tcgctccctg gctaatggaa ccggaaagcg caatgtgttt tagtagcgaa
    12781 atgcggcgcg ggtcggcgaa taatttttgt tggagcttaa gggtgagaag gatttcggcc
    12841 tgcataacaa tgtcctggca aaagtcttat tgtgacggaa aacgaacgcc acgcaaagct
    12901 gaccgcacaa aaggggagtg cttttctgtg cttagcggtt agaatagtct catgactata
    12961 tctggagttg accatgttag agttattaaa aagtctggta ttcgccgtaa tcatggtacc
    13021 tgtcgtgatg gccatcatcc tgggtctgat ttacggtctt ggtgaagttt tcaacatctt
    13081 ttctggtgtt ggtaaaaaag accagcccgg acaaaatcat tgattccctg aatgcccgct
    13141 tagtcgggca ttttcttttt ctcaacttcc ttttctgccg atattttttc tcatctacct
    13201 cacaaaggtt agcaataact gctgggaaaa ttccgaactt gccgttatat tgacgcctac
    13261 ataacgttac attaaggggt taccaatggc tcgtaaatgg ttgaacttgt ttaccggggc
    13321 ggcactctct ttcgctgttg ctggcaatgc actggcagat gaagggaaaa tcacggtgtt
    13381 cgccgccgca tcactgacta acgcaatgca ggacattgct acgcagtata aaaaagagaa
    13441 aggcgtggat gtggtttctt ctttcgcttc gtcatctact ttggcccgtc agattgaagc
    13501 gggtgcgcct gcggatctgt ttatttctgc cgatcagaaa tggatggatt atgcggttga
    13561 taaaaaagcg atcgatacgg cttcgcgcca gacactgctc ggcaatagcc tggtcgttgt
    13621 agcaccgaaa gccagcgagc agaaagattt caccatcgac agcaaaacca actggacttc
    13681 gctgctgaat ggcggtcgcc tcgcggttgg tgatccggaa catgtccctg ctggcattta
    13741 tgcgaaagaa gcactgcaaa aactgggcgc atgggatacg ctctccccga aactggctcc
    13801 ggcggaagat gttcgtggcg cgctggcgtt ggtcgaacgc aatgaagcgc cgctgggtat
    13861 tgtttacggt tctgacgcag ttgccagcaa aggggtaaaa gtggttgcca ccttcccgga
    13921 agattcacat aaaaaagtgg aatatccggt tgctgttgtg gaagggcata acaatgcgac
    13981 agtgaaagca ttttacgatt atctgaaggg gccgcaggct gcggaaatct ttaaacgtta
    14041 cggatttaca accaagtaat gatactgacc gatccagaat ggcaggcagt tttattaagc
    14101 ctgaaagttt cttccctggc tgtgctgttt agcctgccgt ttgggatctt ttttgcctgg
    14161 ttactggtac gttgcacgtt tccgggcaaa gctctgctcg acagtgtact gcatctaccg
    14221 ctggtgttac cgcccgtggt cgtcggttac ctattattag tttcgatggg acggcgcgga
    14281 tttatcggtg aacgtctgta tgactggttt ggtctttctt tcgcgtttag ctggcgcggt
    14341 gcggttctcg ctgccgctgt catgtctttt ccgctgatgg tgcgggcaat tcgtctggcg
    14401 ctggaaggtg ttgatatcaa actggagcag gccgcaagaa cactgggggc cgggcgctgg
    14461 cgcgttttct ttactatcac gttaccgctg accttaccgg gaattattgt tggtacggtg
    14521 ctggcattcg ctcgttcgct cggtgaattt ggcgcaacca tcacctttgt gtcgaacatt
    14581 cctggtgaaa cgcgaaccat tccttctgcc atgtataccc tgatccagac ccctggcggg
    14641 gaaagtggcg cggcgagact gtgtattatt tctattgcgc tggcgatgat ctccctgttg
    14701 atttcagaat ggctggccag aatcagccgt gaacgggcgg ggcgctaatc atgctggaac
    14761 tgaatttttc ccagacgttg ggcaatcatt gcctgacgat taatgaaacg ctgcccgcca
    14821 atggcatcac cgctattttt ggtgtctctg gtgccggaaa aacctcgctg attaacgcca
    14881 tcagtgggct gacacgtccg caaaaagggc ggattgtcct caatggtcgg gtactaaatg
    14941 acgccgataa aggtatctgt ctgacgccag aaaagcgtcg cgtaggctat gtgtttcagg
    15001 atgcgcggct gtttccgcat tataaagtgc gtggcaattt gcgctacggc atggcgaaaa
    15061 gcatggtcaa tcagttcgat aagctggtgg cgcttttagg cattgaaccg ttgcttgatc
    15121 gtttaccagg cagcttgtcc ggaggcgaaa aacagcgcgt ggcgattggt cgggctttgc
    15181 tgacagcacc ggaattgctg ttgctggatg aaccgctggc gtcactggat attccgcgta
    15241 aacgcgaact gttgccttat ctgcaacgtc tgacgcggga aatcaacatt ccgatgttgt
    15301 atgtcagcca ttcgctggat gagatcctcc atctggcaga cagagtgatg gtactggaaa
    15361 acggtcaggt gaaagccttt ggcgcgctgg aagaagtctg gggtagtagc gtgatgaatc
    15421 cgtggctacc gaaagagcaa caaagtagca ttctgaaagt gacagtactt gagcatcatc
    15481 cgcattacgc gatgaccgcg ctggcgctgg gcgatcagca tttgtgggtc aataagctgg
    15541 aagaaccgct gcaagctgcg ctacgcatcc gcattcaggc ttccgatgtc tcgctggttt
    15601 tacaaccgcc gcagcaaacc agcattcgta acgtattgcg ggcaaaagtt gttaatagtt
    15661 atgacgacaa cggccaggtg gaagtggagc tggaagtcgg cggtaaaacg ctgtgggcgc
    15721 gtatcagccc gtgggccagg gatgaactgg cgatcaaacc tggcctgtgg ctgtatgcgc
    15781 aaattaaaag tgtgtcgata accgcctgat taaatcaggt agctataaat gaactgggca
    15841 atgctgtcgg tggtgttatc accaatcaca atgttggcgc gcgcttttac tgcgtcatcg
    15901 gcgttgccca tcgccacgcc tgtaccagcg gcttccagca tactaatatc attgaagtta
    15961 tcgccgaacg ccacgacgtt ttccatcgac cagccttgtg cctcaaccca tttcgtcaaa
    16021 cgtttacctt tgctgttgcc gccgcgtgca atatcaacct gatcgtgcca ggaccattca
    16081 cactccagtc ccagttcatg ttcgacatgc ttaccaaaat gctgcaattg cggcaggtca
    16141 tcgtgcgtca gggcgaactt ccatacggcg ttaacttgtt gcgccgtttc agccagagaa
    16201 gcgacttgtg tgaaagtcgg acgctgttcc ggcggcaggg tttgcgccca gttagacgtg
    16261 cgaatgacat gcccggtcgg gtgctcatag accattgcat catcgacata catcagaccg
    16321 tgaatgtggt gttcattcag catctcaatg agttgcaggg ctttattaac gggcattggg
    16381 tccgcttcca gcacggtttt tgcatgataa tcatacaaat aggtgccatt acagcaaata
    16441 gcaggtgtat ccagcgccag cgcctgataa aaaggatgaa tagcgacgtg atggcgacct
    16501 gtgacgatga ttaattgata gcctgcttcg cgagcgcggg ccagggcttc tatcgatgat
    16561 ggaagcaagg tctttttcgg ggtcaataag gtgccgtcta agtcgagagc aatcacgcgt
    16621 gtggtcatgg cgtattccag attaaggtta agaattttcg tctgcgcgaa tggtacaccg
    16681 ataccactat cggacaaaat tctgcgtttt aattcagcat tcaccgccaa aagcgactaa
    16741 ttttagctgt tacagtcagt tgctaaatgc aaaggagcat tcatgaagca aacagtttat
    16801 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
    16861 acgctgacac aggtagtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
    16921 aaacgttacc tctacgttgg tgtccgtccg gagtttcgtg ttctggcgta tcgtattgca
    16981 ccggatgatg gcgcgctgac ctttgctgca gagtctgcgc tgccgggtag cccgacgcat
    17041 atttccaccg atcaccaggg gcagtttgtc tttgtcggtt cttacaatgc gggtaacgtg
    17101 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
    17161 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgttgtgggt tccggcatta
    17221 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
    17281 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaat
    17341 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtatg ggaactgaaa
    17401 gatccgcacg gcaatatcga atgtgtccag acgctggata tgatgccgga aaacttctcc
    17461 gacacccgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
    17521 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
    17581 aaagaaggct tccagccaac ggaaactcag ccgcgcggct tcaatgttga tcacagcggc
    17641 aagtatctga ttgccgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
    17701 gagcaggggc tactgcatga aaaaggccgc tatgcggttg ggcaggggcc aatgtgggta
    17761 gtggtcaacg cgcactaacc gctgatttaa ccggcgcagt ctctcctgcg ccggtgtatt
    17821 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
    17881 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttcagctgcc ggagaaagca
    17941 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggga
    18001 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
    18061 ccagtcccgc ctccaccagc cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
    18121 ccagcttcca gggcttgtcg gctagctgtt gttctatcaa cagtcggttg ccgctggagg
    18181 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
    18241 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
    18301 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
    18361 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
    18421 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
    18481 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
    18541 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
    18601 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
    18661 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaatgaac
    18721 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
    18781 cggaaaatgg aagcgtcgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
    18841 caacttaaat taatgaaaaa atgt
//
LOCUS       DADSZD010000084        18697 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.084, whole genome shotgun sequence.
ACCESSION   DADSZD010000084 DADSZD010000000
VERSION     DADSZD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.084"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..754
                     /locus_tag="J1Z28_002660"
     CDS             <1..754
                     /locus_tag="J1Z28_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBA7717483.1"
                     /translation="FKSWFVDFDPVIDNALDAGNPIPEALQARAELRQKVRNSTDFKP
                     LPAEIRSLFPSEFEETELGWVPGGWETNRLENILELAYGKALKKTERIEGDYPVYGSG
                     GVDGSHNEFLVKGPGIIVGRKGTVGSLYWENKDFYPIDTVFYVKPKKYFSLVYCYQLL
                     KTLGLENMNTDAAVPGLNRNNAYRLDVITPTQTIIAQYTNIVQTFRYKMDSNNNEIDN
                     LTNLRDTLLPKLISGELSLEDLPDLTTDTEAA"
     gene            849..4112
                     /locus_tag="J1Z28_002661"
     CDS             849..4112
                     /locus_tag="J1Z28_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HBA7717484.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDNRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQAAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGSNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDETLYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            4327..4665
                     /gene="symE"
                     /locus_tag="J1Z28_002662"
     CDS             4327..4665
                     /gene="symE"
                     /locus_tag="J1Z28_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBA7717485.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESKLMQSLRQVCKLSARKQKQVQEF
                     IGVISSKTPR"
     gene            complement(4708..4872)
                     /locus_tag="J1Z28_002663"
     CDS             complement(4708..4872)
                     /locus_tag="J1Z28_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HBA7717486.1"
                     /translation="MEFHENKAKAPFTGIVQLWQAVRTWWLKKQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            5049..6461
                     /locus_tag="J1Z28_002664"
     CDS             5049..6461
                     /locus_tag="J1Z28_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA7717487.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQILETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQVARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYATSSFNVPSTQMAAATFVQEGHYHRHIRRMRQNYQRNMALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(6703..7623)
                     /locus_tag="J1Z28_002665"
     CDS             complement(6703..7623)
                     /locus_tag="J1Z28_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA7717488.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGVDPEW
                     IQKITGLSAEQIQALEQPLPDREPYSWLKS"
     gene            7808..9088
                     /locus_tag="J1Z28_002666"
     CDS             7808..9088
                     /locus_tag="J1Z28_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HBA7717489.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIEKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            9204..10355
                     /locus_tag="J1Z28_002667"
     CDS             9204..10355
                     /locus_tag="J1Z28_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HBA7717490.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            10365..11132
                     /locus_tag="J1Z28_002668"
     CDS             10365..11132
                     /locus_tag="J1Z28_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717491.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTNVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIGRLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRT"
     gene            11129..11386
                     /locus_tag="J1Z28_002669"
     CDS             11129..11386
                     /locus_tag="J1Z28_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBA7717492.1"
                     /translation="MKEYLFLFHSTVGVVQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCLPGEETQWVTPGHTESVYCQQDGGWRCIAHYGVSPR"
     gene            11451..12311
                     /locus_tag="J1Z28_002670"
     CDS             11451..12311
                     /locus_tag="J1Z28_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HBA7717493.1"
                     /translation="MTKSISLSKRIFVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDLIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQESPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDKALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLAGEVIGEMSLTKGSRGLSHNIKQAEGVAMDASGNLYIVSEPNR
                     FYRFTPQSSH"
     gene            12379..13557
                     /locus_tag="J1Z28_002671"
     CDS             12379..13557
                     /locus_tag="J1Z28_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7717494.1"
                     /translation="MPSSTHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GSFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLASALASGLLILAMAWL
                     LPFILVLLIRVLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFDLSSQTLWQGAGALSGMILIALTLLMPSKKHAIVPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAICVLLTLASDSPLLLIISSLGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALASATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13570..14124)
                     /gene="kptA"
                     /locus_tag="J1Z28_002672"
     CDS             complement(13570..14124)
                     /gene="kptA"
                     /locus_tag="J1Z28_002672"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBA7717495.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGTCIRAVQGHSTSQVAIAFIEKTPPQF
                     LYHGTASRFLEAIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GLPFWQAENGVWLTSTVAVEFLEW"
     gene            14374..15057
                     /locus_tag="J1Z28_002673"
     CDS             14374..15057
                     /locus_tag="J1Z28_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717496.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            15054..15515
                     /locus_tag="J1Z28_002674"
     CDS             15054..15515
                     /locus_tag="J1Z28_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717497.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15528..16700
                     /gene="iadA"
                     /locus_tag="J1Z28_002675"
     CDS             15528..16700
                     /gene="iadA"
                     /locus_tag="J1Z28_002675"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBA7717498.1"
                     /translation="MIDYSAAGFTLLQGAHLYAPEDRGICDVLIANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGITSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYSFSLSDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            16765..17676
                     /gene="hypT"
                     /locus_tag="J1Z28_002676"
                     /gene_synonym="qseD"
     CDS             16765..17676
                     /gene="hypT"
                     /locus_tag="J1Z28_002676"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBA7717499.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIEPG"
     gene            17683..18054
                     /locus_tag="J1Z28_002677"
                     /pseudo
     CDS             17683..18054
                     /locus_tag="J1Z28_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(18102..18485)
                     /gene="iraD"
                     /locus_tag="J1Z28_002678"
     CDS             complement(18102..18485)
                     /gene="iraD"
                     /locus_tag="J1Z28_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBA7717500.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
BASE COUNT         4646 a         4640 c         4680 g         4731 t
ORIGIN      
        1 gttcaaatca tggtttgtgg attttgatcc ggtgattgat aacgcgctgg atgcaggaaa
       61 tccaatcccg gaagccctgc aagctcgtgc cgaattgcgt caaaaagtac gtaatagtac
      121 agattttaaa ccgcttccgg cggaaattcg ctcgcttttc ccaagtgaat ttgaagagac
      181 ggagttaggt tgggtgccag gaggctggga aacaaaccga ctggaaaata ttttagagct
      241 ggcttatggg aaagcactca aaaaaacaga aagaatcgaa ggagattatc ccgtttatgg
      301 ttctggtggg gtggatggtt cccataatga gtttcttgtt aaaggacctg gtattattgt
      361 tggaaggaaa ggaactgttg gttcattgta ttgggagaac aaagattttt acccaattga
      421 tacagtattt tatgtaaaac ctaaaaagta cttctcgctt gtttactgct atcagctctt
      481 gaaaacacta ggattagaaa atatgaatac tgatgcggca gtccctgggt tgaacagaaa
      541 caatgcttat cgtcttgacg tcataacacc aacacaaaca atcattgccc aatatacaaa
      601 tatagtacaa acgttcaggt ataaaatgga ttcaaataat aatgagattg acaatctaac
      661 caacctccgc gacaccctgc tcccgaaact catctccggt gagctatccc tggaagatct
      721 tccggatctc accaccgata cagaagctgc ataacgcatt ttgcccctgt aaaatcaggg
      781 gctttctggt aaggttttct actgatacgg gaatgcttac cagaaattag ccagggttgg
      841 agcgcgatat gagtctctct ttcagtgaag caaaattaga acaagcgatc attgaactgt
      901 tacaggatca gggatatcaa cacctgatcg gcgataatgt cccacgttcg agtctcgatc
      961 aggtcattat cgaagacgat ctccgtcatt atttagcggc acgctaccag cctgatggca
     1021 ttactgaaga agagattcag cgactgatca aacagttcac cacacttccc gcctccgatc
     1081 tttatgaaag caacaaaaca ttttgtcgct ggctggcaaa tggttttctg ttcaaacgcg
     1141 acaatcggca acaaaaagat ctctatattg aattgctaga cacccggcat ctacctgccg
     1201 cgctgcgcca gatatttgac gccgaagatg tcccgttgca acaggcggcg gaactcccgc
     1261 cctcctatat taatccgccg cttaaccgga ttaagattgt taaccagctt aaaatctccg
     1321 gcaaagataa tcagactcgt attcctgacg gcattctcta tatcaacggt ctgccactgg
     1381 tcgtctttga atttaaaagt gcggtgcgcg agcaggaagc cagtattggc gatgcctgga
     1441 agcagctctg taaacgctac cgccgggata ttccacaact gtttatctac aacgcgctct
     1501 gcattattag cgatggcgtt aataaccgga tgggcaacct gtttgcgccc tatgaatatt
     1561 tttactcatg gcgcaaagtc accggtaatg aaaaccgtga acaggatgga attccatcac
     1621 tgcactcaat gattcagggg ctgtttcatc cggtacgtct gctggatgtg attaaaaact
     1681 ttatctgctt cccggataaa gccaagcacg aagtaaaaat ttgctgccga tatccgcagt
     1741 actatgccgc ccgcaaactc tattacagca tcaagcaagc gcgtaaacct ttcggtagcg
     1801 gtaaaggcgg cacctacttt ggcgcaacgg gctgtggcaa aagttacacc atgcaatttt
     1861 taacgcgtct tttgatgaag agcgtggagt ttgccagccc gaccattgtt ttgatcaccg
     1921 accgcaccga tctggacgat cagctttctg cgcaaatgtg caacgccaaa aattacattg
     1981 gcgatgacac catcgttccc gttaccagcc gtgaagattt gcgtaatcaa ctggcgggac
     2041 gcaatagtgg cggtgtattc ctgacaacga tccataaatt caccgaagac accgaactcc
     2101 tttctgaacg cagcaatatc atttgcatct cggacgaagc acatcgcagt caggttaacc
     2161 tcgaccagaa agtcatcgtc gataaagaaa gcggaaaagt gcgcaaaact tatggctttg
     2221 cgaaatacct gcacgattca ctgccaaacg ccacctatgt tggctttacc ggcacaccga
     2281 ttgacgcgac gctcgatgtc ttcggtgagg tgatcgacag ttacaccatg accgaatccg
     2341 ttcaggatga aatcactgta cgcatcgtgt acgaaggccg tgcggctaaa gtgatcctgg
     2401 actccagcaa actggaagaa gtcgaaaagt attacgaaga gtgcgcaaac gcaggctcca
     2461 atgagtggca aatcgacgaa agcaaaaaag ccaccgcaac catgaatgcg gttctgggtg
     2521 atgaagaccg attaaaagcc ctcgcggaag attttgccaa acattatgaa aaacgcgtag
     2581 ccgaaggttc caccgtaaaa ggcaaagcca tgtttgtttg tgccagccgt gaaattgcct
     2641 gggatttcta ccgccagctt aaagctattc gccctgcctg gtttgaagtg aagcaagcac
     2701 ctgatggcgt cgtcctgacg gaacaggagc agaaagagtt accgccttct gaaatggtga
     2761 agatggtcat gacgcgcggt aaagatgacg acgagacgct ttatgattta ctgggcacaa
     2821 aagaatatcg taaagagctg gataagcagt tcaaaaacgc taaatcgaat ttcaaaattg
     2881 ccattgttgt tgatatgtgg ctgaccggtt ttgacgtccc ggaactggat accatctata
     2941 ttgataagcc cttacaaaaa cataacctta tccagactat ttctcgcgtt aaccgtaaac
     3001 tggaaggcaa aagcaaagga ttagtggtgg actacatcgg cattaaaagt cagatgaacc
     3061 aggcactggc aatgtattcc cgcattgatg ccaccaactt tgaagatatt cagcaatcgg
     3121 taacagaagt taaaaaccat ctcgatttgt tggggcaagt cttttacgac tttgacagtc
     3181 gggattattt tagtggtgag ccacaagcgc aattatcctg cctcaaccgc gcggcggaat
     3241 tcgttctgcg tacccagaaa gttgaacgtc gttttatggg actggttaaa cgcatgaaag
     3301 ccgcctacga cgtctgctgc ggcagcgaag cattatcaca gacagaacgt gactatattc
     3361 actattatct tgctgttcgt tcaattgttt tcaaactgac gaaaggtgac gcaccggatg
     3421 ttacccagat gaatgcacgc gttcgtgaaa tgattgcaga agcgttaaaa gctgatggcg
     3481 tagaagaaat ttattttctt ggcgataaaa aagcggaatc catcgatatt tttgacgaag
     3541 attatctggc gcgaattaac aagatcaaac ttccggcaac gaagatccag ttattacaaa
     3601 aattactgga aaaagcaatc agcgacttca agaaagtgaa ccagttgcaa gggattaact
     3661 tcacccgccg cttccaggcg attatagatc gttataatga gcggcgagaa gatgatgtac
     3721 tcaacggtga agaattcgat acattcagtc aggaaatgac tgatattatc tatgatatta
     3781 aaaccgaaat gggcacctgg gccgatttag gcattgatat tgaagaaaaa gcctttttcg
     3841 acattcttgc tcatatgcgc gataaatatc agttcaccta tgacgatgaa aaaatgctgt
     3901 cactggcaaa agagatgaaa agcgtggttg acaacacatc gaaatatcct gactggagta
     3961 aacgcgatga tattaaagcg aaactgaaag ttgaacttat tctgcttcta cacaagcata
     4021 agttcccgcc agtagcgaat gatgatgttt atatgggtgt gctggcacag gcagagaatt
     4081 ttaaagaaca tcatatgagt gctttaaatt aattatgaag gccggataca ctttgttatc
     4141 cggccattct gaattaacgt taatttctct tccagatctt tccagtaacg cttttattat
     4201 tttctatttt tagctggact ttccccatat ttactgatga tatgtccagg tatttagcgc
     4261 ggtgcggatg tgcgccaaca cacccgcacc gctaatcaca atccctattt caaaggaata
     4321 gtaatcatga ctgacacgca ttctattgca caaccgttcg aagcagaagt ctctccggca
     4381 aataaccgtc aattaaccgt gagttatgcg agtcgctacc cggattacag ccgtattccc
     4441 gccatcaccc tgaaaggtca gtggctggaa gccgccggtt ttgccactgg cacggcggta
     4501 gatgtcaaag tgatggaagg ctgtattgtc ctcaccgccc aaccacccgc cgccgaagag
     4561 agcaaactga tgcagtcact gcgccaggtg tgcaagctgt cggcgcgtaa acaaaagcag
     4621 gtgcaggagt ttattggggt gattagtagc aaaacgccac gctaagattc tctccccttc
     4681 cccacacggg aaggggaatt ttcctggtca ctccacatcc tccctgcgta acccaatatc
     4741 cctcaaccgc tcatcactca tctgctgtaa cacgcgccgg gtctgctttt tcagccacca
     4801 ggtcctcacc gcctgccaga gttgtacgat gccagtaaac ggcgctttgg ctttgttttc
     4861 gtgaaattcc atctgctgcc tcctcatctt tcagaggctt catcatccgc cttttggcag
     4921 ttcacaatac agattcaaat attactttta tttaacatac agacagatta aaacagcatc
     4981 tgcatccggt ttttaagcct catctgtacc ggtttttctc tctgtatggt ccaaagaagg
     5041 aatacagcat gacgcgttat caacatctgg cgactctgct tgccgagcgg attgagcaag
     5101 ggctgtatcg tcacggggag aaattgccgt cagtgcgcag cttaagtcag gagcacggcg
     5161 tcagcatcag taccgtgcag caggcgtatc agatactgga gacgatgaag ctcatcactc
     5221 cgcagccgcg ttcgggttat tttgtcgcac aacgtaaagc ccagccgcca gtaccgccga
     5281 tgacgcgtcc ggtgcagcgc ccggtagaga ttacccagtg ggaccaggta ctggatatgc
     5341 tggtagcgca cagcgacagt tccattgttc cgttaagcaa aagcacgccg gatgtcgaaa
     5401 cgcccagcct gaaaccgctg tggcgggagc ttagccgggt agtgcagcat aatctgcaaa
     5461 ccgttctcgg ttatgacttg ttagccggtc agcgagtgtt gcgagagcag gttgcccgcc
     5521 tgatgctcga cagcggctca gtggtcaccg ccgatgacat cattatcacc agcggctgcc
     5581 ataactcgat gtcgctggcg ttaatggcgg tatgtaaacc gggcgatatt gtcgcagtcg
     5641 aatccccctg ttattacggt tcaatgcaga tgctgcgcgg tatgggcgtg aaagtgattg
     5701 aaatcccaac cgatccagaa actggcatca gcgttgaagc gctggaactg gcgctggaac
     5761 agtggccgat taaaggcatc attctggtgc caaactgtaa taatccgctg ggatttatta
     5821 tgccggacgc gcgcaaacgg gctgttctct ctctcgctca gcgtcatgat attgtgattt
     5881 ttgaagatga tgtctacggc gaactggcaa cggagtatcc gcgcccgcgg accattcatt
     5941 cctgggatat cgacgggcga gtgctgctgt gcagttcgtt cagtaaaagc attgcgcctg
     6001 gcctgcgcgt gggttgggtc gcgccgggac gttatcacga taaactgatg catatgaaat
     6061 atgccaccag cagctttaat gtgccgtcca cgcaaatggc ggcagcaacg tttgtgcagg
     6121 aaggtcacta tcatcgtcat atccggcgga tgcggcagaa ctatcagcgc aatatggcgc
     6181 tttatacctg ctggatacgg gaatattttc cctgcgaaat ctgtattacg cgcccgaaag
     6241 gcggattttt actgtggata gaattgcctg aacaggtcga tatggtctgc gtcgcgcggc
     6301 aactgtaccg catgaaaatc caggtggcgg caggctcgat tttctcagcg tccggcaaat
     6361 accgaaactg tctgcgcatc aactgcgctt tgccgctcag cgaaacctac cgcgaagcac
     6421 tgaagcaaat tggcgaggcc gtgtatcggg caatggaata aattcgaaat agtggcgaag
     6481 cacctgattt cctctcacga atcgcctgta actcgtttta tctttatagc gaaatcgcca
     6541 cgttccggca tggcttaggc aaagtgtgct ttgttcatgc cggatgcggc gtgaacgcct
     6601 tatccggcct acaaaccgcg caaattcaat agattgcaga gataatgtag gtctgataag
     6661 cgtagcgcat caggcagctt tgcgtttgtc atccgtctct gattagctct tgagccatga
     6721 ataaggctca cggtcaggca agggctgctc taatgcctgt atttgctctg ccgaaagtcc
     6781 ggtaatcttt tgtatccatt caggatctac cccattctgc aaaaacaatc gaagaatgcg
     6841 ctgttcccct tttatatatc catcattatg aattcgctct gcaatcgtca ttattcgctc
     6901 cctttgttgt ggcagtcgac gggtaatttc actgataaac tcattaaaac gcgcttcatc
     6961 accagtcagt aaaatgtaat ttaacagcgc agttatctgg ctgtcattag cacactctgt
     7021 aaccagtaat actaccagtt ggtcaataag ccccatcaga tcgcgctggc gaatatgctt
     7081 ttggatcaac tccaacaggg cgactctgcg atgctgcaca atctcgtcgt ctggcacgac
     7141 agtgatatcc accagcggga acgcggcggt ataaagcttc cgtgcggtag tcgggtcggc
     7201 aaattcgtcc agccagcaca aagaccaggg ataaggacta cggctaccgt gataaaacag
     7261 catcgggatg accagcggta gctgccggcg tttatcatgt tcgatatggc gctgcatcac
     7321 cgccatggaa tatcgcatca gacgaaaggc catatgaata tcctcacggc tctgatgttc
     7381 aatcactaca taaatatagc catcgccttc acgggtcttt accgaccaca gaatatcgga
     7441 atgtagcgcc cgcaattttt catcgacaaa gctggcggat tccagtttta agctatcgag
     7501 atcgcacagt tcacgtaaat ctttgggtaa gtggatctcc atgaaatccc gcgcggtgtc
     7561 agagtgtgtg agaaaggatt taaataatgc gtcatgcggc gtgctggtcg taaagtttgt
     7621 catggcgatc cgtcacctgt agataagatg gcccaacttt agcgactaac aaagccattc
     7681 acacctggct atttcctatt ccccgaggcg gtttccaggc tatttttatt cttctgcaaa
     7741 cgatacattt ttatggaaga tatctcgctt tttgcgtttt cttaccgtca ggctctattc
     7801 ttacgatatg aataaactca ttgaactcag acgcgccaaa atgttggcgc tctctttact
     7861 gcttatcgcc gctgctacct ttgtcgttac gctgtttttg ccgcccaatt tttgggtgag
     7921 cggtgtgaag gcgattgctg aagcggcgat ggtcggcgcg ctggcggact ggtttgcggt
     7981 ggtggcgctg tttcgccgcg tgccgattcc gatcatttct cgtcatacgg cgattatccc
     8041 gcgtaataaa gaccggattg gcgaaaatct cggccagttc gtgcaggaaa aatttctcga
     8101 tacccaatcg ctggtggcat tgattcgacg tcacgaaccg gcgttgttga ttggcaactg
     8161 gtttagtcag ccggagaacg cccgccgcgt tggtcagcat ctgttacaga tcatgagcgg
     8221 ttttctcgaa ctgaccgatg atgcgcgtat tcagcgcctg cttaagcgtg cggtccaccg
     8281 ggcgatcgat aaggtcgatc tttccggcac cagtgcgttg atgctggaga gtatgaccag
     8341 aaacgatcgt catcaggtgc tgctcgatac gctgatcgca caactgatcg ccctgcttca
     8401 gcgcgataaa tcacgcaagt ttattgccca gcaaattgtt cgctggctgg agagcgagca
     8461 tccactgaaa gccaaaattt tgcccactga atggctgggc gaacatagcg cggagttggt
     8521 ttctgacgcg gtgaattctt tgcttgatga tatcagccgc gatcgtgcgc atcagatccg
     8581 ccatgcgttt gatcgcgcta ccttcgccct gatcgagaaa ctgaaaaacg atccggaaat
     8641 ggcagcgcga gccgatgccg taaaaagcta tctgaaagaa gatgaagctt ttaaccgcta
     8701 tctcagtgaa ttgtgggggg atttacggga atggctgaaa gcggatatca acagtgaaga
     8761 ttctcgtgtg aaagaacgta tcgcgcgggc ggggcaatgg tttggcgaga cgttaattgc
     8821 cgatgatgcc ctgcgggcgt cgttaaatgg tcatctcgaa caagccgcac accgcgttgc
     8881 gcctgagttt tccgcattcc tgactcgcca tatcagcgac acggtaaaaa gctgggacgc
     8941 acgggatatg tcgcggcaaa tcgagttaaa tatcggcaaa gatctccagt ttatccgtgt
     9001 caacggtacg ctggttggcg gttgtattgg acttattttg tatttgctgt cgcagctccc
     9061 ggccttgttc cccctcggca atttatagaa atccatgaaa aactaagtgg ctaaccgcga
     9121 gagagatcaa ataaaccaca ctaaaagaat ggtaataatg tggcctcttt tcattatctc
     9181 ccgtggtacg gggaaggaaa atcatgtcac ttgtcaccga tctacccgcc attttcgatc
     9241 agttctctga agctcgccag aaaggctttc tcaccgtcat ggatctcaag gagcgcggca
     9301 ttccgctggt tggcacctac tgcaccttta tgccgcaaga gatcccgatg gcagccggtg
     9361 cggttgtggt ttcgctatgt tccacctctg atgaaaccat tgaagaagcc gagaaagatc
     9421 tgccgcgcaa cctctgcccg ctgattaaaa gcagctacgg cttcggcaaa accgataaat
     9481 gcccctactt ctacttttct gatctggtgg tcggtgaaac cacctgcgac ggcaaaaaga
     9541 aaatgtatga atacatggcg gagtttaagc ccgttcatgt gatgcaactg ccaaacagcg
     9601 ttaaggacga tgcctcgcgt gcattatgga aagccgagat gctgcgcttg caaaaaacgg
     9661 tagaagaacg ttttgggcac gagattagcg aagatgctct gcgcgatgcc attgcgctga
     9721 aaaaccgcga acgtcgcgca ctggccaact tttatcattt ggggcagtta aatcctccgg
     9781 cgcttagcgg cagcgacatt ctgaaagtag tctacggcgc aaccttccgg ttcgataaag
     9841 aggcgttgat caatgaactg gatgcaatga ccgcccgcgt tcgtcagcag tgggaagaag
     9901 gccagcgact ggacccgcgc ccacgtattt taatcaccgg ctgcccaatt ggcggcgcag
     9961 cagagaaagt ggtgcgcgcg attgaagaga atggcggctg ggttgtcggt tatgaaaact
    10021 gcaccggggc gaaagccacc gagcaatgcg tggcggaaac gggtgatgtg tacgacgcgc
    10081 tggcggataa atatctggcg attggctgct cctgtgtttc gccgaacgat cagcgcctgc
    10141 aaatgctcag ccagatggtt gaagaatatc aggtcgatgg cgtagttgat gtgattttgc
    10201 aggcgtgcca tacctacgcg gtggaatcgc tggcgattaa acgtcatgtg cgccagcagc
    10261 acaacattcc ttatatcgct attgaaacag actactccac ctcggatgtc gggcagctca
    10321 gcacccgtgt cgcggccttt attgagatgc tgtaaggagt ggcagtgaca tattcgattg
    10381 gcattgattc cggctcaacc gccaccaaag ggatcttact ggcagacggc gtgattacgc
    10441 gccgtttcct cgttccaaca ccctttcgcc cggcaacagc aattactgaa gcctgggaaa
    10501 ctctgcgcga agggttagag acaacgccgt ttctgacgct caccggctac ggacggcaac
    10561 tggtggattt tgccgataaa caagtgacgg aaatctcctg tcacgggctg ggcgcacggt
    10621 ttcttgagcc agcaacgcgc gcggtaatcg acatcggtgg tcaggacagc aaagtgattc
    10681 agcttgatga tgacggtaac ctgtgcgatt tcctgatgaa tgacaaatgc gcggcgggca
    10741 ccgggcgttt cctggaagtg atctcgcgca cgcttggcac caacgtcgaa cagctcgaca
    10801 gcattaccga aaatgtcacg ccgcacgcca tcacgagtat gtgcacagtg tttgccgaat
    10861 cagaagtgat cagcctgcgc tctgcgggcg tagcgccaga agcgattctc gccggagtga
    10921 ttaacgcgat ggcgcggcgg agcgccaatt tcattggccg cctctcttgt gaagcgccga
    10981 ttctgttcac tggcggcgtg agtcactgtc agaggtttac ccacatgctg gaaagccatc
    11041 tgggaatgcc ggtacaaact caccctgatg cgcaattcgc tggcgcaatt ggcgcggcgg
    11101 tgattggtca gcggcagaga aaacgcacat gaaagagtat ctgtttttat ttcactcaac
    11161 cgtcggcgtt gtacaaacgc gtaaggcgtt acaggctgcg ggtatgactt ttcgcgtcag
    11221 cgatattccc cgtgatttac gcggcggctg tgggttatgc atctggctga cctgtcttcc
    11281 tggcgaagaa acacaatggg taacccccgg tcatacggaa tcagtttatt gccagcagga
    11341 tggtggctgg cgctgcattg cacattacgg ggtttctccg cgttaaggat tcattaagat
    11401 cgctacgtat acttctcgat atcttattga tttgccttaa agggattacc gtgacaaaaa
    11461 gtatttcgtt aagtaaacgc atcttcgtta ttgtgattct ttttgccatt gtggcagtgt
    11521 gtactttctt cgtgcaatct tgcgcccgta aaagcaatca tgcagcatca tttcagaatt
    11581 accacgccac cattgacggc aaagaaattg caggcataac gaataatatc tcttcgctga
    11641 cctggtcggc acaaagcaat actcttttta gcactatcaa caaacctgcc gccattgttg
    11701 aaatgaccac caatggtgat ttgatccgca caattcctct ggattttgtt aaggatctcg
    11761 agacgatcga atacattggc gataatcagt ttgtcattag cgatgagcgc gattacgcca
    11821 tttatgtgat ttcactgacc ccaaactcgg aagtaaaaat cctcaaaaaa ataaaaatcc
    11881 cgctgcagga atccccaacc aattgcggtt ttgaaggatt ggcctattct cgccaggatc
    11941 acactttctg gttttttaaa gagaaaaacc cgattgaggt ctacaaagtt aacgggctat
    12001 taagtagcaa tgaattgcac atcagcaaag acaaggcact acaacggcaa tttactttag
    12061 atgatgtgtc cggcgcagaa tttaaccaac aaaaaaatac gcttctggtg ctgtctcatg
    12121 aatctcgcgc actccaggaa gtaacgctgg ctggcgaggt tatcggtgaa atgtcactaa
    12181 ccaaaggcag tcgaggtttg tcgcataaca tcaaacaggc cgaaggggtt gcgatggatg
    12241 cctctggtaa tctttatatc gttagcgaac caaatcgttt ttatcgcttt acccctcaat
    12301 catcgcatta acaggctggc atgttttacc tttttgttat taacttgtta acaacaaaaa
    12361 tgacaaacaa ggttaaacat gccttcatcc acgcatcccg tagaacgctt ttctttcagc
    12421 accgcgcttt tcgggatgct ggttctgacc ttaggtatgg gtttaggccg cttcctttat
    12481 acgcctatgt tgcccgtcat gatggcggaa ggatcgttct catttagcca gctctcgtgg
    12541 attgccagcg gcaactatgc ggggtatctg gctggcagtc tgctattttc gtttggcgca
    12601 tttcaccagc catcgcgcct gcgcccattt ctgttggctt ctgccctggc gagcgggttg
    12661 ttgatcctcg caatggcatg gttgctgcca tttattctgg tgttattgat tcgcgtcctg
    12721 gcgggtgtcg ccagcgccgg aatgctgatt tttggttcga cgctgattat gcagcacacg
    12781 cgccatcctt ttgtgctggc agctttgttt tctggcgttg gcattggcat cgcactgggc
    12841 aatgaatatg ttctggcagg cctgcatttt gacctctcct cgcaaacgtt atggcaaggc
    12901 gccggggcgc tttctggcat gatactgatt gcacttacgc ttttaatgcc ctcgaaaaaa
    12961 cacgccatcg taccaatgcc attggcaaaa acggagcaac agataatgag ctggtggtta
    13021 ctggctattc tgtacggcct ggcgggattt ggttatatca tcgtcgcgac ttacctgccg
    13081 ctcatggcga aagatgcagg ctcaccattg ttaaccgccc atctctggac gttagtcggc
    13141 ttatcaattg tgcctggttg ctttggctgg ctatgggcag caaaacgttg gggggctttg
    13201 ccctgcctga cggcgaattt gctggtgcag gctatctgtg tgctgcttac tctcgccagc
    13261 gactcgcctc tcttgcttat catcagcagt cttggttttg gcggtacctt tatgggcacg
    13321 acatctctgg tgatgactat cgcccgccaa ctgagcgtac cgggaaatct caatctttta
    13381 ggctttgtga cgctcattta cggcattggg caaatcctcg gcccggcgct gaccagtatg
    13441 ctcggcaatg gaacatcggc gcttgccagc gccactctct gcggcgcggc ggcgttgttt
    13501 attgctgcgc ttatcagcac agtgcaatta tttaagctac aagtggtcac ttcatgaggg
    13561 aagtttccct taccactcaa gaaactcaac cgcaaccgtt gatgtcagcc agaccccgtt
    13621 ttccgcttgc cagaaaggga ggcctcgttt cgccatttcc tgtgctttga cggttaaaat
    13681 aaccggcgat ccgtgtcttg ccccgacttt gcgggccgtt gcttcatcag cagagagatg
    13741 cacgtaatgg cgctcccctg caatcagccc ctgttttttt atcgcctcaa ggaaacggct
    13801 tgccgtaccg tggtagagaa attgtggcgg tgttttttcg atgaaggcaa tggcgacctg
    13861 cgaagtcgaa tgcccctgaa cggcgcgaat acacgtacca tcactggaat aactaaagcg
    13921 ctttttatcg ctggtggcga ccacagtatc cagtaatgcg cgggttaacc gcttgcccgc
    13981 tttttgtgca cagagtatga gcttatcgat atccgcccat ccttcacggt ccagcacgat
    14041 gccaatcgcc tctggtttgt gtcggaggac aaaacttaaa aacttgctcg tatccgccag
    14101 ctctttttcg ttgtactttg ccatctcgtt ctcgtccctg gattttctgt ttttatttac
    14161 ttatgttatt gtcactgggt ttattaattt actgcaactc aatccagcgc agaacctttt
    14221 ccccatagcg taatttttcc gctatgcaaa aaacgcatag ccagaaacat acttgcattt
    14281 tccccatacc cacatggctg ataaggtttt tcagctccag gaattttcct ggctcaagca
    14341 acacgcaaac acaaaaaaac aatggggatt gttatgacac aacaagggga tgctgtagcc
    14401 ggggagctgg cgacagaaaa agtgggcatt aaagggtatc tggctttttt tctcaccata
    14461 atcttttttt ccggtgtttt ttccgggaca gacagttggt ggcgcgtttt tgatttttcc
    14521 gtgctgaacg gatcgtttgg gcaactgcct ggtgcgaacg gagccaccac ctcgtttcgc
    14581 ggcgcgggtg gcgcgggggc taaagatggc tttctctttg cgctggagct ggcaccgtca
    14641 gtgattctct ctctggggat tatttcaatt accgatggtc ttggcggatt acgcgccgcg
    14701 cagcagttaa tgacgccagt gctaaaaccg ttgttgggca ttccaggcat ctgttcactg
    14761 gcgctgattg ctaacctgca aaacaccgat gccgccgcag gtatgaccaa agaactggcg
    14821 caggaagggg aaattaccga acgtgataaa gtgatattcg ccgcctatca aaccagcggt
    14881 agcgccatca tcaccaacta cttctcttcc ggcgtcgcgg tgtttgcctt tctgggcacg
    14941 tccgtaatcg tacctttagc cgtcatcctg gtgtttaaat ttgtcggagc caacattttg
    15001 cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc aaggagcgca agcatgacaa
    15061 ctcaggtccg caaaaacgtc atggatatgt ttattgatgg cgcgcgccgt ggctttacta
    15121 tcgctaccac caacctgctg ccaaacgtgg tgatggcctt tgtcatcatc caggcgctga
    15181 aaattactgg cctgctcgac tgggtgggac atatctgtga acccgtaatg gcgctgtggg
    15241 gattgcccgg tgaagcggca accgtccttt tagccgcatt gatgagcatg ggcggcgcag
    15301 tcggtgttgc cgccagtctg gcaaccgctg gcgcattaac cggccacgat gtcaccgttt
    15361 tactgcccgc gatgtattta atgggcaacc cggtgcaaaa tgtcggtcgc tgcctgggga
    15421 cagccgaagt gaatgccaaa tattatccgc atatcattac ggtctgcgtg attaacgcat
    15481 tgctgtcgat ctgggttatg cagcttattg tttaacaagg agttaccatg attgattatt
    15541 ccgcagccgg ttttaccctg ctgcagggag cgcatttgta tgcgccggaa gatcggggaa
    15601 tttgcgatgt cctcatcgct aacggcaaaa ttattgccgt tgccagcaac atcccttctg
    15661 acattgtacc ggactgcacg gttgtcgatc tcagcgggca gatcctctgc ccaggtttta
    15721 ttgatcaaca cgtccatttg attggtggtg gcggcgaagc aggtcccacg acacgcacgc
    15781 cggaagtggc gttaagtcgt ctgacggaag cgggcatcac gtcagtggtt ggcctgctgg
    15841 gcaccgactc tatctctcgc cacccggaat ccctgctcgc caagacccgt gcgctcaatg
    15901 aagaaggcat cagcgcctgg atgctgaccg gcgcttatca tgtcccttcc cgcactatta
    15961 cgggttccgt ggaaaaagac gtggcgatta tcgatcgcgt gattggtgtg aaatgcgcca
    16021 tctctgatca ccgttctgcc gcaccggacg tttatcacct ggctaatatg gcggcggaat
    16081 cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac cgtgttccac atgggtgaca
    16141 gcaaaaaggc gttacagcca gtctatgacc tgctggaaaa ctgcgatgtg ccgatcagca
    16201 agctgctgcc gacccacgtt aaccgcaacg ttccgttgtt tgagcaggcg ctggagttcg
    16261 cgcgcaaagg cggcaccatc gacatcacca gcagcattga cgaaccggtc gcccctgccg
    16321 aaggtattgc ccgcgccgtt caggcgggta ttccgctggc acgcgtcacc ctcagctccg
    16381 acggcaacgg tagccagccg ttctttgatg acgaaggaaa tttaacccat atcggtgttg
    16441 caggttttga aacattgctg gaaaccgtgc aagtgttggt caaagactat agcttcagcc
    16501 ttagcgatgc cctgcgcccg ctcaccagta gcgtagccgg tttccttaac ctgaccggga
    16561 aaggcgaaat tctgccaggc aatgacgcag acttactggt catgacgcct gaattgcgca
    16621 ttgagcaggt atacgctcgc ggcaaactga tggtcaaaga cggcaaagcc tgcgtgaaag
    16681 gaacgtttga aacggcataa acggttgtca gccgcccgaa tggacgtaga atgcccgcaa
    16741 gggcggctga cagagtataa cgtaatggat gactgtggtg cggttttgca taacattgaa
    16801 accaaatggc tttatgattt tctgaccctg gaaaaatgtc gcaatttttc ccaggcggca
    16861 gtcagtcgca acgtctcgca accagcattc agccgccgca tccgtgcgct ggaacaggcg
    16921 attggtgttg aattgtttaa ccgccaggta acgccgctgc aactctcaga acaagggaaa
    16981 atcttccatt cgcagatccg ccatctgttg caacagttag agagcaatct ggcagagctg
    17041 cgtggcggca gcgattacgc gcaacgtaaa atcaagattg ccgctgcaca ctctctttcc
    17101 ctcggtctgt taccgtccat tatcagccag atgccgccgc tctttacctg ggcgattgaa
    17161 gccattgatg ttgatgaagc ggtcgataaa ctgcgtgaag ggcaaagtga ctgtattttt
    17221 tcctttcacg acgaagattt gctggaagca ccgtttgacc acattcgctt atttgaatct
    17281 caattattcc ccgtctgcgc cagtgacgaa cacggagaag cactttttaa cctcgcgcag
    17341 ccacactttc cgttactgaa ttacagccgc aactcctaca tggggcgatt gattaatcgc
    17401 actctgacgc gccacagtga gttgagtttc agcacctttt ttgtctcttc gatgagcgag
    17461 cttttaaagc aggttgccct cgacggatgt ggaattgcct ggttgccgga gtatgccatc
    17521 caacaagaaa ttcgcagcgg gcagctcgtt gtactgaatc gggacgaatt agttatcccg
    17581 attcaggcct atgcataccg gatgaatacc cgcatgaatc cagttgccga acgcttctgg
    17641 cgtgaactgc gcgagctgga gattgaacct ggctgaaagt tttctgcaga aaatcttccg
    17701 gcgctgggaa ttatgattga ggttcctgcc gcagtcatga ttgcagacaa attagccagt
    17761 gaagtcgatt tcttttccat cggcaccaac gatctgacgc aatatattat ggcggcagac
    17821 agaggaaata gcaccgtcgc gaaactggtc gattattgta atgacgcggt gattaacgct
    17881 attgctatgg tgtgtcaggc cggacgtaat aatgaaattc cggtcagtat gtgcggcgaa
    17941 atggctggag atatccagca aacagcacga ctgctcacca tggggattga taagctcagt
    18001 gccagtccgt cccggcttcc ggccttgaaa gcagcaatca gaacctccca ctaattgtga
    18061 cctaacgcga tacgtcttgc gcgtatcgcg tttcccgtca tttactgcgg catcgtgcga
    18121 tgccagctcc ccttctgcca gaccaggtca aacgttagcg gctcatcatc gtggtacagc
    18181 atcatctcca gactaatggc caactcgtgg caataggctt ctttcagatt gacctgcaat
    18241 gccgcaatac gtggctcatg aaataaaagc gcggaacgta actggcggca ataccaactc
    18301 tggagaagct tgtcgctctg attatgccct tgccactcag gcatgcctga aacaagtaat
    18361 attggtagcg gtgaatgggg agaactaaaa agttgaaaga gatcccggca tacggaatca
    18421 cgcagcgata acgtattgtt tcctgtactt tcaggtaata ccgtctgaac tgattggcgc
    18481 atcattttgc gcactcctga cgtttagtgg catctgtatc ctgccctggc tgccggacac
    18541 cactgggttg atgctcatat tccataatca ttcctttatg ctcattgcgt ccctggcact
    18601 ttcataaggg atttataaga tccgccaaag ataaaacact gtacagaaaa gaatatccac
    18661 tgacttttaa tttaaaaagg tatcatttga caaaagt
//
LOCUS       DADSZD010000085        18661 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.085, whole genome shotgun sequence.
ACCESSION   DADSZD010000085 DADSZD010000000
VERSION     DADSZD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.085"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            13..984
                     /locus_tag="J1Z28_002679"
     CDS             13..984
                     /locus_tag="J1Z28_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HBA7717501.1"
                     /translation="MKKINFAFIILFLFSLPLIIFYQPWVNALPPTPRHASPEQLEKT
                     VRYLTQTVHPRSADNIDNLNRSAEYIKEVFISNGARVTAQDVPITGGPYKNIVANYGP
                     ADGPLIIIGAHYDSVSSYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLV
                     AYASEEPPFFRSDEMGSAVHAASLERPVKFMIALEMIGYYDSTPGSQDYPSPAMSWLY
                     PDRGDFIAVVGRMQDINAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHD
                     IPAVMITDTAFYRNKQYHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1087..1590
                     /locus_tag="J1Z28_002680"
     CDS             1087..1590
                     /locus_tag="J1Z28_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBA7717502.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1657..3114)
                     /gene="nuoN"
                     /locus_tag="J1Z28_002681"
     CDS             complement(1657..3114)
                     /gene="nuoN"
                     /locus_tag="J1Z28_002681"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBA7717503.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3121..4650)
                     /gene="nuoM"
                     /locus_tag="J1Z28_002682"
     CDS             complement(3121..4650)
                     /gene="nuoM"
                     /locus_tag="J1Z28_002682"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBA7717504.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4814..6655)
                     /gene="nuoL"
                     /locus_tag="J1Z28_002683"
     CDS             complement(4814..6655)
                     /gene="nuoL"
                     /locus_tag="J1Z28_002683"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBA7717505.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6652..6954)
                     /gene="nuoK"
                     /locus_tag="J1Z28_002684"
     CDS             complement(6652..6954)
                     /gene="nuoK"
                     /locus_tag="J1Z28_002684"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBA7717506.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6951..7505)
                     /gene="nuoJ"
                     /locus_tag="J1Z28_002685"
     CDS             complement(6951..7505)
                     /gene="nuoJ"
                     /locus_tag="J1Z28_002685"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBA7717507.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7517..8059)
                     /gene="nuoI"
                     /locus_tag="J1Z28_002686"
     CDS             complement(7517..8059)
                     /gene="nuoI"
                     /locus_tag="J1Z28_002686"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBA7717508.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8074..9051)
                     /gene="nuoH"
                     /locus_tag="J1Z28_002687"
     CDS             complement(8074..9051)
                     /gene="nuoH"
                     /locus_tag="J1Z28_002687"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBA7717509.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9048..11774)
                     /gene="nuoG"
                     /locus_tag="J1Z28_002688"
     CDS             complement(9048..11774)
                     /gene="nuoG"
                     /locus_tag="J1Z28_002688"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBA7717510.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11827..13164)
                     /gene="nuoF"
                     /locus_tag="J1Z28_002689"
     CDS             complement(11827..13164)
                     /gene="nuoF"
                     /locus_tag="J1Z28_002689"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBA7717511.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13161..13661)
                     /gene="nuoE"
                     /locus_tag="J1Z28_002690"
     CDS             complement(13161..13661)
                     /gene="nuoE"
                     /locus_tag="J1Z28_002690"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBA7717512.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13664..15466)
                     /gene="nuoC"
                     /locus_tag="J1Z28_002691"
     CDS             complement(13664..15466)
                     /gene="nuoC"
                     /locus_tag="J1Z28_002691"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBA7717513.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15560..16222)
                     /locus_tag="J1Z28_002692"
     CDS             complement(15560..16222)
                     /locus_tag="J1Z28_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017490251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HBA7717514.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16238..16681)
                     /gene="nuoA"
                     /locus_tag="J1Z28_002693"
     CDS             complement(16238..16681)
                     /gene="nuoA"
                     /locus_tag="J1Z28_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBA7717515.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17311..18249)
                     /gene="lrhA"
                     /locus_tag="J1Z28_002694"
     CDS             complement(17311..18249)
                     /gene="lrhA"
                     /locus_tag="J1Z28_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBA7717516.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4663 a         5154 c         4814 g         4030 t
ORIGIN      
        1 aataatttgc atatgaaaaa aataaatttt gcttttatta tattatttct gttttcactc
       61 cctctgatta ttttttacca accctgggtg aatgcattac cgccaacgcc ccgacacgca
      121 agtcctgagc aattagaaaa aacagttcgt tatcttacac aaacagtgca tccacgtagc
      181 gccgacaata ttgataatct gaataggtcc gcagagtata taaaagaggt ctttatcagt
      241 aacggtgcca gagttaccgc gcaggatgtc cccattacgg gtggccccta caaaaacatt
      301 gttgctaatt atggtcctgc cgatggaccg ctgattatta ttggtgcgca ctatgactct
      361 gtcagtagtt atgaaaacga tcaattgacc tatacgccgg gcgcggatga taacgccagc
      421 ggcgtggcag gattactcga actggcacgt ttgttacagc agcagactcc agggattggc
      481 gtccaactgg tcgcctatgc gtcggaagaa ccgcccttct ttcgtagcga tgaaatgggg
      541 agcgcggtac atgcagcttc gcttgagcgt ccagtgaaat tcatgatagc gctggagatg
      601 attggttatt acgactctac gcctggtagc caggattacc catccccggc aatgtcctgg
      661 ctttatcccg atcggggaga ctttattgcc gtggtcggca gaatgcagga tatcaacgcc
      721 gttcgtcagg taaaagcggc attgttgtca tcccgggatt tatctgttta ttctatgaat
      781 gccccaggtt ttattcccgg tattgatttc tctgatcacc ttaattattg gcaacacgat
      841 attcccgccg taatgattac tgacaccgct ttttatcgta ataaacaata ccacttgccc
      901 ggtgatatcg cagacagatt aaattatcag aaaatggctc aggtagtgga tggagttaca
      961 accttgttat acaacagtaa ataataaaaa tttctcgagg cggatccaga aaaagcaaca
     1021 tgcgcatttt attttctttc gtctgtttta agatgcgaaa catgaaattc aacggaggaa
     1081 aaataaatgg gaatgattgg ctattttgca gaaattgact cagaaaagat taaccaactg
     1141 cttgagtcta ctaaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc
     1201 cgactggata ttgataaacg ctgggatttt ttacactttg ggctgacagg cacctctgct
     1261 tttgatcccg cgaagaatga cccgctgagt cgcgccgtgc ttggtgaaca cagcctagaa
     1321 gatggcatag atggcttcct gggattgacc tggaatcagg agttagccgc gacaatcgac
     1381 cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat
     1441 gaaatggaga tttatccggg agtaaccttt tcagaagagt tagaggggca actctttgct
     1501 tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg
     1561 aatcatgcgc tgacggttat tgttgggtga tggtgtcagg gcaccaaaaa aaaccgccga
     1621 acatgtcggc ggctttctga cttacaaact gactgtttac atcagcggca ttgctaaccg
     1681 cacaatgcta atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagagat
     1741 cagcaccacg ataccgcctg cgctgtactg ccagtttgat ggtgcatcgc gacccggttg
     1801 ttccggagcg tgcaggtata agctcaccgc cacgcgcagg tagtagtaaa ggccgattgc
     1861 cgaaccaaca accacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag
     1921 cacgtagaac ttaccgataa agcccagcgt cattgggata ccagccagtg aaagcatcat
     1981 caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag
     2041 ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc
     2101 gaggctgctg aacagataac cgaccaggta aacccctacc gcttccatcg acatctcgcc
     2161 ggtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc
     2221 gagcagacgt ttgatattgg tctggctcag cgccatcagg ttaccgaaga tgatggaggc
     2281 aaaggcgata atcgccagca ctacgcgaat cgcttcactg tcacccaccg gtgcgtaaag
     2341 gaacaaacgc atcaccacgc cgaagatagc gattttgctc gccgtcgcca ggaaagtgga
     2401 aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagaga
     2461 gagtttgaaa ccgaggccaa caatcatcag gccaaaacct gccagcaaca gcggctcgtt
     2521 gagcataccg tcgccgaggt ttttacccag tgcgacaaac gacaggtcgc cagactgcgc
     2581 atacaccagc gccataccga acagcaggaa agaagacgct gcggcagaaa ggatggtgta
     2641 tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag
     2701 cggcaaagag atcagttcga taccgaggaa cagagacgcc agatggttgg cattcgccag
     2761 caggatcccg cccagcgcgg caattaacac caacaggtag aactcgtcct tgttgtcgtt
     2821 atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag
     2881 cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc
     2941 cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt
     3001 agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa
     3061 gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat
     3121 ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga
     3181 gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag
     3241 caacaggatc ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg
     3301 gcttttcgct ttgccgaagt aagcgcgatg cagcatcgcc agcgaataaa cagatgcaaa
     3361 gaccagccca aaggtagaga taacggtaat caccgggaca acctggaaac tgccgaacag
     3421 aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa
     3481 gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat
     3541 gtcgcgggta tggatacgtt cataaagctg accacaaaga ataaacagac ccgccgccga
     3601 caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta
     3661 gatagcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat
     3721 atcggtctgg gcgaaggcca tccacgcacc atagaagatg ccgataacac ccagccacat
     3781 agcgattggc gcgaactctg ccgacgcgtt tgggaacagc ggtagggaga aacgcagcag
     3841 accgtaagcg gcggttttca gcaagatccc cgcgaggtcc acggaaccgg cggtcggagc
     3901 ctgtgagtgc gcatccggta accagccatg cagcggaacc accggcattt tcactgcgaa
     3961 ggcgatgaag aagcccagca tcaacaggta ttccacgccg ttagacatcg gcgtattcag
     4021 cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacga aaaccagcgc
     4081 caggatggcg atcaacatca ccagaccact cgcctgggtg taaatgaaga acttggttgc
     4141 cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta
     4201 catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa
     4261 cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt
     4321 ttcgatctct ttccacgaac agagtaccgc cagcacaccg agcagaccgg tcagcacgac
     4381 catcagcaac gacagaccgt caatggcgag atgaatagag ataccaaaac gcgggatcca
     4441 cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc
     4501 gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtta ccagcgcgat
     4561 ccagcgcggc accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccgataaa
     4621 gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt
     4681 caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg
     4741 tcggatgcgc cgcgaagacg gcttatccga cctacgttct tatatgccca ggggcaatcc
     4801 cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc
     4861 tcatggatgc cacataccag cgcagataac cgttttcgct taacagcaga cctttacctg
     4921 caaagcggga aaggacagcc gggatgttca tcattgagtt cagcggatcg cgtttcagca
     4981 accaggcaat acccaggaac ggcttgacga acactttgtc gtacagccag tcaaagcccc
     5041 aggcgttgta ccaccaggta cccagcagac ggcccggcgc actgttggcg atggaggtca
     5101 ccagagtacg tttacccagc cacagccagg cagccagcag aatgccgacc accgcgacca
     5161 cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagctccgtc gtttgcggaa
     5221 gcacgccctg cagcggcggt acaatcagtg caccaacgaa ggtggaaagg atcagcagca
     5281 caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc
     5341 cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca
     5401 gacctgccac catcagattg atatgtccat tcgccatcgc tcccgcgagg atctcatcct
     5461 tactgaagaa gcccgcagtg accagcggca gtgccgatag tgctgcgccg cccaccagga
     5521 agcagagata aaccagcgga atagatttac gcagaccacc catcttaaag atgttctgtt
     5581 cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg
     5641 cgtgggtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca
     5701 tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg gtctgtacca
     5761 gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatacccacc agatgcagaa
     5821 cttccggcgt catcaggaac aggccgtggg tacgggcgat caggtagaca cccgcggtta
     5881 ccattgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcggcaa
     5941 gccatgtctg caacggcaac tgagcagatt taccgaccgc accgcccagc agcatcagcg
     6001 tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt
     6061 cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagcgcgaaa gcgaggaaga
     6121 cgtcacccac acgggtcacg acgaacgctt tcattgccgc tgcgccattc ttcggatcgg
     6181 tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca
     6241 tcagcagcag gttgtcggca agcaccagaa ctaccatgct ggcgatgaac aggttggtgt
     6301 aagcgaagaa gcgagagtag ccctcttcac cgcgcatata ccaggaggcg tacatgtgaa
     6361 taaggaaacc cacaccagtg accaccgaga gcatggtcag cgacaggccg tccagcacca
     6421 ggttaaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg
     6481 cctgctcacc gttagcgaag aagtcaacgc cgataaaggc agttaccagc gccgccaggc
     6541 ccacagagcc aacgccgacg atcgccgaga cgttttcaga ccagcgccca cgggagaacg
     6601 ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg
     6661 catctcactt actgaatcga tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag
     6721 cgcaaggccg atactcgctt ccgccgccgc gaggctgatg gcgagaatgt acatcacctg
     6781 accgtcggtc tggccccagt agcttccggc gaccacaaag gccagcgcag aggcgttaat
     6841 catgatttcc agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa
     6901 gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc
     6961 tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc
     7021 tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat agaagccagt
     7081 tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc
     7141 gtaccgtcga taccttgatc gttaacaccg agaatggcgt aaacaatcac caccagcatg
     7201 atggctgaca aaattgccgg accaatccac acctgcggtt tcagccactg gcgttcctgt
     7261 tcgatttctg aaccgcccag gttgagcatc atcaccacga acacgaacag caccataatg
     7321 gcacccgcat agacgataat ttccagcgca ccggcgaagt aagcgcccag tgagaagaac
     7381 accccagaga tcgccagcag cgaaataatc aggtacagca gcgcgtgtac cggattggta
     7441 tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac
     7501 tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct
     7561 ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt
     7621 ccgggtattt gcccggaccg gagatcagca gatcctcttt ctcgtaaacc agatcctggc
     7681 gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt
     7741 cttcgcacag accacagaaa atgcagcgtg agaagttgat gcggaaaaat tccggatacc
     7801 agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg
     7861 cgcagaggtt acaggctacg caacgctctt cgccgtccgg atcgcgggtc agaacgatac
     7921 gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt
     7981 tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta
     8041 acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc
     8101 cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat
     8161 tacctggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa
     8221 gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc
     8281 gaagaacagc gtcaccatca gtgcagagat ggtcacaatc ccgatgtatt cgcccacgaa
     8341 gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca gttcctgctc
     8401 ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga tggcaaaggt
     8461 aataaaacca aagaattgcg gtatgacgtt ccacacatgc gcctggctat tgacgatgtc
     8521 ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac
     8581 ttcgtagctc agggtctgcg cagaagcacg catcgccccc agcagcgaat atttgttgtt
     8641 acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca tcaggaagaa
     8701 caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca caattgcaaa
     8761 ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cacgatccga
     8821 gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa ccagctggag
     8881 cgaaccgccc cagccaacac ggttaggtcc ataacggttc tggaacagac ccagcaggcg
     8941 acgttcgcca aagctcatga atgccccaca ggtgactacc accagcagga tcaccaccgc
     9001 tttaaggacg gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc
     9061 tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga catacccatc
     9121 ggcaagccca cctgccctgc tgtcagccct tcggcgattt caaccggcag cgtgaccgtg
     9181 ttgccatcgt aactaaagga gacgcgtgta ccagcgttca cacccaactt cgcggcatcc
     9241 gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt
     9301 gacaattcat cgctgccaaa caggtggtag tacggcgcga tacgccattt cccgtcctgc
     9361 ggctggaagc gtgccggtac gctggtgaag taatccagac cgttttcgct ggtttcaaac
     9421 agacgcacgc ccggatcgcc aaaacgcagt ttgccgccca cttcgtcctg gaatttgttc
     9481 cacgcctgcg gggagttcca gcctggcgcc caggcaaacg gcacttgcga acggtgcgcg
     9541 gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggctga
     9601 cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctata gcgatgcggt
     9661 tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata
     9721 cctgccagtt ccgggatttt cgccacaaca gcgtcaatca cgtggtcgag ctgcgtccag
     9781 tccacttcac ggctcagcaa ggtgctgtgc agcgagtgta accaacgcca gctttccagc
     9841 atgacagttt tgctgtcgta ataagccgga tcgtaaacct ggaagaaacg ttgggcacgg
     9901 ccttcgttgt taatcaccgt accgtcgctt tcagcaaagc tggcagcaga aagtaccaga
     9961 tgggcgtttt ccataatcgc tgtgcgttga tggtcaacca ccatcaccag cggcgcttta
    10021 gccagcgcag cattcacgcg ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc
    10081 accaccgcgt cggcgcgtcc ggtttccagt tcggttaacg cttcttcaag cgatccaccg
    10141 cccataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat accgacgtca
    10201 gcgccgcgac ctttcagggc tttcgccacg ttagccgcag cctgaatcac ttcagcgcta
    10261 ccggcgttcg tcccggagat aatcaacggt ttcttcgcac ctgccagtgc ctgcacgatg
    10321 acgtcgattt tgctttgcag ctcaggttca ataccgtcaa ccgctggcgc ggagttatcc
    10381 agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc
    10441 cacgccgcga tatcatccag acgggtgtca tcaacgttgg taacaaacag cggatgcttc
    10501 gcacgttggc cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgcc
    10561 gccatttcgc gcgctttacc tttcaccgcc tgacgaactg ccagcgcgac gcgcgcgccg
    10621 gtctgggtaa cgtcttcgcc cagcaccagt accgcatcgt aagattcgat ttcgcgcaga
    10681 gccggagtat aaatgccgcc ttcacgcagc actttcagcg ccagttgcag acgttcctgc
    10741 tcaccgtgag cgataccggt gtagaagttt tcttcgccca ccagctcacg cagcgcaaag
    10801 ttgctttcca cgctggcgcg cggagaacca atgccgatca ctttcttcga ctgacgcaga
    10861 atatctgctg cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc atcgccacga
    10921 cgctgtactg gctgacgcgg acgatctttc aggttgacgt agccgtaacc gaaacgacca
    10981 cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat acgacgcagt
    11041 tcgccataac gctcgccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc
    11101 ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtgaat
    11161 acaccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag cgtgccgtct
    11221 tccgggcgac cgaagtagac gttgtcgtgc gcaccgtaaa cgcccaggtc cgtgccgtca
    11281 gcgtaatctt tgtagtaacg cacgcagcgg tagcaggcga tgcagcggtt catttcgtga
    11341 gagatgaatg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg
    11401 aagctatgtc cggtcatcac agtcatatcc tgaagatggc agttaccgcc ctcttcacat
    11461 accggacagt cgtgcgggtg gttggtcatc aaccactcaa ccacgctttc acggaactgt
    11521 ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac
    11581 atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg
    11641 caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga
    11701 gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt gccgtctaca
    11761 tgaattgtag ccattagcat gcttccagtt ttctcagtca gagactgagc gttaatcgaa
    11821 attcggttac cagcgctctt tcagcaggtt tggctgaatc ccattaatca aatgggtatt
    11881 gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg
    11941 taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg
    12001 acacagttgc tcaagtgttt cgatatcgcc cggctggcct tcgccacgct ccagcgcacg
    12061 cagaattttc acgctccacg gcagaccgtc gcggcatggc gtacaccagc cgcaggactc
    12121 acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac
    12181 cgccatcgcc agcgccgtac ccagacggct tcccgcttta ccgatacttt cgaattccat
    12241 cggcaggtca aggtgcgctt cggtcaggaa gtcagtcccc gcgccgcctg gctgccaggc
    12301 tttgaatttc agaccgtcac gcataccacc ggcgtagtct tcgaggatct cgcgtgcggt
    12361 ggtgccgaac ggcagttccc acagacccgg atttttcacc cgaccagaga agcccatcag
    12421 cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag
    12481 gatcgccgga acgttacaca gggtttcgac gttgttaacg caggttggtt taccccatgc
    12541 accggaggtt gccgggaagg gcggtttcga gcgtgggtta gcgcgacgcc cttccaggga
    12601 gttgattaac gctgtttctt caccgcagat gtagcgccct gccccggtat ggacgaacag
    12661 ttcgaaatcg aaacctgttc ccataatgtt tttgccaagc agacccgctt cggtggcttc
    12721 ggcaatggca cgacgcagat tcactgccgc ttcgatatat tcgccgcgca ggaagatata
    12781 gccacggtaa gctttcagcg caaacgcgga gatgagcatg ccttccacca gcaggtgcgg
    12841 cagttgctcc atcagcagac ggtctttata ggtgcccggc tccatttcat cggcgttaca
    12901 cagtaggtaa cggatattca tggattcgtc tttcggcatc aggctccact tcaggccagt
    12961 cgagaagcct gcgccaccgc gccctttcag accagagtct tttacctgat taacgatttc
    13021 gtccggagac aacccggtca gcgccttacg cgcgccttcg taaccgtttt tgctgcgata
    13081 ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc
    13141 gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg
    13201 gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc acagttcccc
    13261 aggcagcaag ttggcagcag cgtgaagcga ccgtcaaatg tcgtttgccc tggtttgatg
    13321 ttcagttttt tctccagcgc cgcctgaata ccctggtaac cgttgatgtg acagaccacg
    13381 ctgtcgcaat aacggatcac atggcggcct actggctggc ggaagatctg gctgtagaac
    13441 gttgccacgc cttcgacgtc actcgccgga atacccagca catcggcgat cgcgtggatc
    13501 gcgccatccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc
    13561 gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc
    13621 agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg
    13681 acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga
    13741 tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt acggatacgg gtgcgataac
    13801 tcatggtgct gccgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaacca
    13861 tctggaagga ttcattggca ggcatcaccg gaccccacga cacttgcagg aagtgggtga
    13921 tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat
    13981 ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaata cgcagactct
    14041 ggcgcaactc ttctactttc agcattactc gggtgtagca gtcagaaaca ccaccgccca
    14101 ccggaatttc gaagtcgaag ttttcatagc cagaataagg acgcgcctta cgcacgtcga
    14161 agtcgatccc ggtggcacgc aggcccgcgc cggtggtgcc ccactccagc gcctctttcg
    14221 cgccgtaagc ggcaacgccc tgggaacgac ctttcagaat ggtgttttgc agcgccgctt
    14281 tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc
    14341 agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg tgcatacgga
    14401 aaccagtgat tgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca
    14461 ctggcgtcat cgcgccgacg tcctgaataa aggtggagat atacagcagg tgactgttga
    14521 tgcggaacag ttcggagagc attacgcgaa tgacgttaac gcgatccggc acggtgatcc
    14581 cagccagttt ctctaccgcc agcacataag gcatttcgtt aacgcagccg ccgaggtatt
    14641 cgatacggtc ggtatacgga atgtagctgt gccaggactg gcgttcgccc attttctctg
    14701 cgccacggtg gtggtaaccg atgtctggta cgcagtcgac aatctcttcg ccatcaagct
    14761 gcaaaacgat acggaaagca ccgtgcgcag acgggtggtt cggaccgagg ttgaggaaca
    14821 tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg
    14881 cctccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc
    14941 gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc
    15001 gcaggttcgg gtgaccgtcg aatgtaatgc caaatagatc ccaggtttca cgctcatacc
    15061 agttagcgtt cgggaataat ttggtaaagg tcggtacgtg caggtcgttt tctgccagcg
    15121 ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa
    15181 aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt
    15241 caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgcccact tccagtaatt
    15301 gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat
    15361 ccggcccaaa acggttgcgc agttcgccaa tcaccggatc atcaagatga tcgcgggtct
    15421 gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt
    15481 ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaattcc
    15541 ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggta acggcaatgc
    15601 gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc
    15661 caaccaccca ggagagcgga cgacgttctt taccgataga ttcctgcaac agcatcagtg
    15721 cctgcatgta cgcttcagga cgcggcggac agcccgggat atacacatca accgggatga
    15781 atttatcaac gccctgcaca acggaataaa tatcgtacat accgccggag ttggcacagg
    15841 cacccattga gataacccat tttggttcca gcatctggtc atacaaacgc tgaataaccg
    15901 gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag
    15961 cacgcaatac ttcagcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca
    16021 tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc
    16081 agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct tgctccagag
    16141 ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta
    16201 tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac
    16261 tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc
    16321 caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca
    16381 caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt
    16441 caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc
    16501 gggcggagcc gaccgagtcg ataccggatt caaacggaac gttttttgac ctcgcgcgtg
    16561 cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga
    16621 taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca
    16681 ttgcttactc atcaaaagta gcgcctgatt ctctgctctt tacggcagat ggacgccaca
    16741 tcgattcatg gggaggaata aaaaaaacct tacaataact gcagaaacta ttttatacag
    16801 ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa caaaggtttc
    16861 ttcacactga tttacacaat gacaacaaca tttgcatttg gcgccctttc tccccagatc
    16921 attgacgact ccaggaaaat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa
    16981 aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt
    17041 tcttgatctg tgacacgctt ttgtcattct ataacaaaaa tgcagcaaca aattcacgta
    17101 ttttttaaca ttattgtagc aggagatttt tttcaggtga ttatttgtgc gtacggggcg
    17161 tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt ctgcacgata cgaggaaagc
    17221 tttttactaa aaaaaagccg ctggggttta aaataaaccc agcggttcgt ttttacacta
    17281 ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga
    17341 gtcatctcct tccggagcag acattgggct gtattgccac ggattatggt agctctccat
    17401 cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata
    17461 ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca ggtccggact
    17521 catcatctcg accggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc
    17581 cggaagcgtc gaggcgacat aagccagtcg ccagggaata tctgctttat taagcgtcgc
    17641 cagcaccata tcgcgaaacg gacttggatc atccagcaac acaagaggga tcggctcacc
    17701 tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa
    17761 tgccttaaag gtagaaggac gatgggtggt taccatcaaa tccacttcct gggattccag
    17821 catttctgcc atgtaggcat tacgtttaac gcggacatcc agcgctagtt tcggataaac
    17881 cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc
    17941 gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa
    18001 acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt
    18061 gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg
    18121 acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc
    18181 ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc
    18241 acttatcata tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac
    18301 acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg
    18361 aaagtaaaaa tgcatatgag ttgcactaaa aaagtgactc acattgttcc gttataatgc
    18421 ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt
    18481 ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc
    18541 gtacagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag
    18601 agcagggatc cgtcatttat gcaagcattt taccccaaaa accttttatt tataagggtc
    18661 a
//
LOCUS       DADSZD010000086        18591 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.086, whole genome shotgun sequence.
ACCESSION   DADSZD010000086 DADSZD010000000
VERSION     DADSZD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.086"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            126..1265
                     /gene="arnB"
                     /locus_tag="J1Z28_002695"
     CDS             126..1265
                     /gene="arnB"
                     /locus_tag="J1Z28_002695"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBA7717517.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DCNTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1269..2237
                     /gene="arnC"
                     /locus_tag="J1Z28_002696"
     CDS             1269..2237
                     /gene="arnC"
                     /locus_tag="J1Z28_002696"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBA7717518.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2237..4219
                     /locus_tag="J1Z28_002697"
     CDS             2237..4219
                     /locus_tag="J1Z28_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-glucuronic acid
                     oxidase/UDP-4-amino-4-deoxy-L-arabinose formyltransferase"
                     /protein_id="HBA7717519.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFMNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLNWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4216..5106
                     /locus_tag="J1Z28_002698"
     CDS             4216..5106
                     /locus_tag="J1Z28_002698"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBA7717520.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQMAGSH"
     gene            5106..6758
                     /gene="arnT"
                     /locus_tag="J1Z28_002699"
     CDS             5106..6758
                     /gene="arnT"
                     /locus_tag="J1Z28_002699"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBA7717521.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGVCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVVIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAILMAHYALLA
                     AKNNPLTLRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6755..7090
                     /gene="arnE"
                     /locus_tag="J1Z28_002700"
     CDS             6755..7090
                     /gene="arnE"
                     /locus_tag="J1Z28_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBA7717522.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGVAFIIG
                     GIVILGSTV"
     gene            7090..7476
                     /gene="arnF"
                     /locus_tag="J1Z28_002701"
     CDS             7090..7476
                     /gene="arnF"
                     /locus_tag="J1Z28_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBA7717523.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7470..7736)
                     /gene="pmrD"
                     /locus_tag="J1Z28_002702"
     CDS             complement(7470..7736)
                     /gene="pmrD"
                     /locus_tag="J1Z28_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBA7717524.1"
                     /translation="MEWLVKKSCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7846..9201)
                     /gene="menE"
                     /locus_tag="J1Z28_002703"
     CDS             complement(7846..9201)
                     /gene="menE"
                     /locus_tag="J1Z28_002703"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBA7717525.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AMQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFSDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9198..10160)
                     /gene="menC"
                     /locus_tag="J1Z28_002704"
     CDS             complement(9198..10160)
                     /gene="menC"
                     /locus_tag="J1Z28_002704"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBA7717526.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELAETLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGLQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVKAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10160..11017)
                     /gene="menB"
                     /locus_tag="J1Z28_002705"
     CDS             complement(10160..11017)
                     /gene="menB"
                     /locus_tag="J1Z28_002705"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBA7717527.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(11032..11790)
                     /gene="menH"
                     /locus_tag="J1Z28_002706"
     CDS             complement(11032..11790)
                     /gene="menH"
                     /locus_tag="J1Z28_002706"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBA7717528.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPVGVIASLAQILRF"
     gene            complement(11787..13457)
                     /gene="menD"
                     /locus_tag="J1Z28_002707"
     CDS             complement(11787..13457)
                     /gene="menD"
                     /locus_tag="J1Z28_002707"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBA7717529.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPHRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETTLVDAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13546..14841)
                     /gene="menF"
                     /locus_tag="J1Z28_002708"
     CDS             complement(13546..14841)
                     /gene="menF"
                     /locus_tag="J1Z28_002708"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBA7717530.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQRWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKVLRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14920..15225)
                     /gene="elaB"
                     /locus_tag="J1Z28_002709"
     CDS             complement(14920..15225)
                     /gene="elaB"
                     /locus_tag="J1Z28_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBA7717531.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15280..15741)
                     /locus_tag="J1Z28_002710"
     CDS             complement(15280..15741)
                     /locus_tag="J1Z28_002710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7717532.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSETLRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15806..16723
                     /gene="rbn"
                     /locus_tag="J1Z28_002711"
     CDS             15806..16723
                     /gene="rbn"
                     /locus_tag="J1Z28_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBA7717533.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAVPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16844..18586)
                     /locus_tag="J1Z28_002712"
     CDS             complement(16844..18586)
                     /locus_tag="J1Z28_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBA7717534.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKKSQQQQSTTPTDQQVLAA
                     QQAATKVAEQSAAKAAADAKALAQQEVQQYSDKQALLGRLQAAPKYQHAAREKAASQI
                     ANPGTARYQQFDDNPVKQVAQNPLATFSLDVDTGSYANVRRFLNQGLLPPPDAVRVEE
                     IVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSEELP
                     ASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSISG
                     SHKAEINAAIDSLDVEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGIDD
                     PKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVLN
                     SEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGKH
                     ITFLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFPL
                     AFAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEFI
                     RLIGLAKDLDNSQN"
BASE COUNT         4302 a         4992 c         4702 g         4595 t
ORIGIN      
        1 atattagttt cttaaggtta agttaatatt ctatccttaa aattttgctc caaatggcaa
       61 aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg acggatggaa
      121 aaaaaatgtc aggatttttg cctttttcgc gaccagcaat gggcgtagag gaactcgctg
      181 cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat caggcgcttg
      241 aacaagcttt ttgccagttg acgggaaatc agcatgccat cgcggtcagt tcagccaccg
      301 ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa gtgattacgc
      361 cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca acgccggtaa
      421 tggtggatgt cgactgcaat acgctgatgg tcacgcctga agcgatcgaa gcagccatta
      481 caccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca gatattgacg
      541 ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct gcccatgccg
      601 tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt ttttcgtttc
      661 acgccatcaa aaatattacc tgtgcggaag gtggcctgat tgttactgat aatgaaaatc
      721 ttgcccgcca gctgcggatg ctgaaatttc acggtctggg tgtcgatgcc tatgacagac
      781 atacctgggg ccgtgcaccg caggctgaag tcttaacgcc gggctataag tacaatctga
      841 ccgatattaa cgccgcgatt gccctgaccc agttagtcaa attagagcac ctcaacaccc
      901 ggcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc tttcagccat
      961 taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt cgtgtcgatg
     1021 aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa agaggcattg
     1081 gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag cgttttccca
     1141 cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg ccgctgttcc
     1201 cggatatgac caccgccgat gccgaccgcg tcatcacagc ccttcagcaa ctcgcaggac
     1261 aatgagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt cccgtttata
     1321 acgagcagga aagcttaccg gaattaatca ggcgcaccac cgcagcctgt gaatcactgg
     1381 ggaaagagta tgaaatcctg ctgattgatg acggcagtag cgataattcc gcgcatatgc
     1441 tggtcgaagc ctcacaagcg gagggcagcc atattgtgtc tatcttgctt aaccgcaatt
     1501 acgggcaaca ttcagcgatt atggcgggat tcagtcacgt caccggcgac ttaattatta
     1561 ccctcgatgc cgatctccag aatccgccag aggaaatccc ccgtctggtg gcaaaagccg
     1621 atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc tggtttcgta
     1681 aaaccgcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa gcgatgggtg
     1741 actatggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg ttgcactgcc
     1801 atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt gccattgaaa
     1861 ttccagtgca ccatgccgag cgtgagtttg gtgaatccaa atacagcttt atgcgcctga
     1921 ttaatttgat gtacgacctg gtgacctgtc ttaccacaac gccgctacgt atgctgagtc
     1981 tgctcggcag cattattgcg attggcggtt ttagcattgc ggtgctgctg gtgattttac
     2041 gcctgacctt cggaccccaa tgggcggcag aaggcgtctt tatgctattt gccgtgctgt
     2101 ttacttttat tggcgctcag tttatcggca tgggattgct cggtgaatat atcggcagga
     2161 tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc cgtccatcca
     2221 gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg gatgcctcgg
     2281 tattgaagcc ctgctggctg ccggttacga aattagcgcc atttttaccc atactgataa
     2341 ccccggtgaa aaagcctttt atggttcggt tgcccgactg gcagcggaaa gaggcattcc
     2401 agtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg cccaactatc
     2461 accagaggtg attttctctt tttattatcg ccatcttatt tgcgatgaga ttttgcagct
     2521 cgctccgaga ggtgcattta atctgcatgg ttcgctgtta ccaaaatatc gtggtcgcgc
     2581 gccgctgaac tgggtgctgg tgaacggtga aacggaaact ggcgttacat tgcaccgaat
     2641 ggtgaaacgt gccgatgccg gggccattgt ggcacaactg cgcgttgcca ttgcgccaga
     2701 cgatatcgcc attacgctgc atcataaatt gtgccatgcc gcgcgccagc tactggaaca
     2761 gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg aaaacgaagc
     2821 cacctgtttt ggtcgcagaa cgccggatga cagtttcctc gaatggcata agccggcatc
     2881 cgtactgcac aacatggtgc gtgccgttgc cgatccgtgg ccgggtgcct tcagctatgt
     2941 tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca gcaaagcaca
     3001 gccggggagc gtgatttctg ttgcaccact gctgattgcc tgtggcgatg gcgcgctgga
     3061 aatcgtcact ggacaggcgg gcgacggcat tactatgcag ggctcgcaat tagcgcagac
     3121 gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgtgccg cccgacgccg
     3181 tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga cagaacgcct
     3241 gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg cgataagccg
     3301 ttttatgaat catccgcatt ttcactttgt cgaaggcgat atcagtattc attccgaatg
     3361 gattgagtat cacgtcaaaa aatgtgatgt cgtcttgcct ttggtggcga tagccacgcc
     3421 gattgaatat acccgcaacc cgctgcgcgt atttgaactc gatttcgaag agaatctgcg
     3481 cattatccgc tactgcgtga agtaccgtaa gcgaatcatc ttcccgtcga cttcagaagt
     3541 ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa tcgtcggccc
     3601 ggtgaataaa ccacgctgga tttattcggt gtctaaacaa ttacttgatc gagtgatctg
     3661 ggcctatggc gaaaaagaag gtttacagtt caccctcttc cgcccgttta actggatggg
     3721 gccacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta ttacgcaact
     3781 cattctcaat ctggtagaag gttcaccgat taagctgatt gatggcggaa aacaaaaacg
     3841 ctgctttact gatattcggg atggtatcga ggcgttatac cgcattatcg aaaatgcggg
     3901 aaatcgctgc gatggcgaaa ttatcaacat tggcaatcct gagaacgaag cgagcattga
     3961 agaactgggg gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc attacttccc
     4021 accgtttgcg ggctttcgtg ttgtcgaaag tagcagctac tatggcaaag gatatcagga
     4081 cgtagagcat cgtaaaccga gcatccgcaa tgcccgccgc tgcctgaact gggaaccgaa
     4141 aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca ccgttgatct
     4201 tacggataaa ccatcatgac caaagtcggc ttacgcattg atgtcgatac ctttcgtggc
     4261 acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat tcaggccagc
     4321 atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact ggtgaagcca
     4381 cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg ggatatttta
     4441 ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga tatcattcgt
     4501 gaagcggcta aacatcacga agtcggcctg cacgcctggg atcaccatgc ctggcaagcc
     4561 catagcggta attgggatcg gcaaacaatg atcgacgata ttgcacgcgg ccttcgcacc
     4621 ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgctggctg gcgtgctgac
     4681 caacaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga ttgtcgtggg
     4741 gcgatgccgt ttcgtccatt gctcgaatca ggaacccctg gcactgcgca aattccggtg
     4801 accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga ttttaacggt
     4861 tggttactca accgcatcca gcgagataaa ggcacgccgg tttataccat tcatgccgaa
     4921 gttgaaggct gcgcttatca gcataatttt gtggatctcc tgaaacgcgc agctcaggaa
     4981 ggcgtgacat tttgcccttt aagcgaactg ttatcaggga cgttgccgct cggacaagtt
     5041 gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat ggcgggtagt
     5101 cactgatgaa atcggtacgt taccttatcg gcctcttcgc atttattgcc tgctattacc
     5161 tgttaccgat cagcacgcgt ctgctctggc aaccagatga aacgcgttat gcggaaatca
     5221 gtcgggaaat gctggcatcc ggcgactgga ttgttcccca tctgttaggg ctacgttatt
     5281 tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta tttggcgcga
     5341 ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc gcgctggtga
     5401 cctggtttac tctgcgctta tggcgcgata aacgtctggc tttactcgcc acagtaattt
     5461 atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat ccgtttattg
     5521 ccttctggct ggtggcggga gtgtgcagct tctggctggc aatgcaggca cagacgtgga
     5581 aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg gtgatgacca
     5641 aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta gcaacgcaaa
     5701 aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc gtactgacgg
     5761 ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcat tattttttct
     5821 gggttgagca tattcaacgc tttgcactgg atgatgccca acatagagcg ccgttctggt
     5881 actacttgcc ggtcgtcatt gccggtagcc tgccgtggct gggattactc cccggtgcac
     5941 tgtacgctgg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttattg agctggacga
     6001 taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat attctttcct
     6061 gctttgcacc tctggcaata ctgatggcgc attacgcgtt gctggcagca aaaaataatc
     6121 ctctgacgtt gcggattaat ggctggatta atatcgcttt tggcgtcact ggcattattg
     6181 ccacatttgt ggtctccccc tggggaccaa tgaacacacc ggtgtggcaa accttcgaga
     6241 gctataaagt cttttgtgcc tggtctattt tttcgctatg ggcatttttc ggctggtaca
     6301 ccttaacaaa cgtcgaaaag acctggcctt ttgccgcgct ttgcccgctg gggctggcgt
     6361 tgctagtagg attttcaatt cctgacagag tcatggaagg aaaacatccg caattttttg
     6421 tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat agcgtcggtg
     6481 ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat cgccagacag
     6541 gtgagttgaa atatggcctt aattatccgg atgcaaaagg gagatttgtc agcggtgatg
     6601 agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc gtgctttcgg
     6661 ttgaccgcga tgaagatatc aatagtctcg ccattccgcc cgcagatgtc atcgatcgtc
     6721 aggagcgtct ggtgctaatt cagtatcggc ccaaatgatc tggctaacat tagtctttgc
     6781 cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct ttgcggcggt
     6841 aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt gtcttggcct
     6901 tgccatggtg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg cctacccgat
     6961 gttaagtctg aattttgtct gggtaacgct ggctgcagta aaactgtggc acgagccggt
     7021 atcgctgcgt cactggtgtg gggtggcgtt cattattggc ggcattgtga tcctcgggag
     7081 tacggtgtag tgggcctgat gtggggatta ttcagcgtca ttatcgcctc cgctgcgcaa
     7141 ttaagcctgg gttttgcggc gagtcatctg ccgccaatga cgcacttatg ggattttatc
     7201 gcggcgctgt tagcttttgg cctggatgcc cggatactgt tactcgggct gctgggttat
     7261 ctgctttccg ttttttgttg gtataaaaca ctacataaac ttgcgctaag caaagcctat
     7321 gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact tccaggctgg
     7381 gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag cgggttgatg
     7441 ctgattttcc tgcccacaac aaaacaacgt tactgagttt tccctgccgc tttacactgc
     7501 cgttcccact catcggggct ataacagctt gcagaaagga ctttcaccgt gtgcagcttt
     7561 tcacgattaa tacagtaaag cgcattctgc aaaggcgaga gtaaatcccc cactttcacg
     7621 gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc gcagagcata
     7681 attacatgtc tgttatcttg tttattgcag cacgattttt tgaccagcca ttccattgca
     7741 ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa actctttatc
     7801 aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt gctggcgttg
     7861 cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca gctccggcgg
     7921 cagagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca cccattcgtc
     7981 aagatcaacg ctttgctggt catactccac caccgctacc ggccgatgac caaactcctt
     8041 gtcggcaacg ggaacgataa acacctgcaa aaccgcagga tgtgcagcaa taacacgctc
     8101 gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattatcta aacgtccgac
     8161 aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtggcgtacc agccttcgtc
     8221 attaactagt ggaacccgtt gcccgttacg ccagtaacct tctgccatac tggcagcccg
     8281 cagccacact tcatcattaa cgattttcac ttcccgaccc ggcagcggcg aaccaacgtc
     8341 tgccagaccg tcggcttctt tcgcacacac cgtggaggca aactcggtca gaccatagcc
     8401 gcaaaagcac cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg ggatagccgc
     8461 gccgccaagt aaaaccgctt tcagggagac ggaactacgg ttaaccagca aacgccagag
     8521 ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atctgctcca atggctgttt
     8581 atcacgtacc gtcatccgcg cgccagcgta taaccagcgc cacataattc cctgaccgga
     8641 gacgtgaaac agcggcaaag aaagcaacca gtcatcgtga tcgctaaacg gaattagcga
     8701 taacacccct tcagcgctgg caagatgggc ctggtaagta tgaacagcgg ctttcggcaa
     8761 gccagtggag ccagaggtta acgtcattga gcacagacgc gtcggctgcc acgccgcggc
     8821 atgtgcgcct tcaaccagct gaatgtgcag cgaagctaac gccggaaacg tgttttcccc
     8881 ttccggcacc agcgcaaatt gcagcgtaag attgggcagc aattcctcaa gcagcggttg
     8941 cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg ccagccaggc
     9001 cagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct caaccacacc
     9061 ctgcatcgca aagctggagg ctaattcatc gacgcgagca caaagctcgc gccagttgag
     9121 ttgctcgtca ttgagacgta aggcgatggc ttctccccgc acttgccgcc agtgacgcca
     9181 cggccagtca gagaagatca taacagccgc tccagtgcat caacgtccac taacggcagt
     9241 gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt gtccagtccc
     9301 ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag acctaagctc
     9361 gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc cttcacctgc
     9421 tcgcgtactt tctccaggct gcccgtgagc gtgggtttga taactaccgc gcgcacgccc
     9481 tcttcagcta caaaggcaaa atccggctcg cgcaagcttt catcccaggc aatggcaatg
     9541 ccggtttcac gggcaaacgc acgcgaatca tcgcgggttt tgcacggctc ttcgagaaac
     9601 gcgatgcggt ggcgataatc cgggttaacg tatttggcaa actgcaggcc tttcagcggt
     9661 gtccaggcgc ggttcgcgtc aagacgcaaa tgcagatccg gaattgcctc cagcaacaaa
     9721 ttcaccacca tgccgtcgcg cacggcttcg tacaatccca ctttgacctt cgccactttc
     9781 tcgccaggca tatcggcaag tttgaggatc agatcgtcag gatcgccatt acacagcggt
     9841 gccgcacggt aattggccgc ctgcgggagc gtctctgcca gctccgccaa tgcacaactt
     9901 acgccaaagg caacggaagg catctgcggt aattcgcaat cgcctgccag ccagttattt
     9961 acccaggcaa gcagcacact ttgcgcatct tcccaggttt cctgactgaa gcccggcaat
    10021 ggggagatct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacata cagcccgtcg
    10081 cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat ctgccagcgg
    10141 tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca ggctgacgtt
    10201 tctggttgaa agcgttgcga ccttcctgac cttcttccgt catatagaac agcatggtgg
    10261 cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc agcgcagctt
    10321 tcaggcagcg cagcgccatc gggctgtttt gcagcatttc acggcaccaa cggacggttt
    10381 ctttttccag atccgccagc ggtacaacgg tgttcaccag acccatatcc agcgcctgtt
    10441 ttgcgtcgta ctgacggcac aggaaccaga tttcacgcgc ttttttctgc ccaacaatgc
    10501 gcgccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg ccagtctgac
    10561 cgaagatggc attatcagcc gcgatagtca ggtcgcacat catgtgcagg acgtgaccgc
    10621 caccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta cggatctgac
    10681 gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag ccgccgtaat
    10741 cgccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg ccagtcagaa
    10801 tgatcacgcc gatgttgtcg tcataacgcg catctgccag tgcctggatc atctctttta
    10861 ccgtcagagg gcggaaggca ttgcgcacct gcggacgatt aatggtgatt tttgcgatac
    10921 cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg tgccattcaa
    10981 ccggtgcgta aagcattgct tcatcaggat aaatcatatt gtgtccttta gtcagaaacg
    11041 caagatctgc gccagacttg cgattacgcc aacgggattt tcccggtgcg cgttatgtcc
    11101 ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagagcgc ggaatttgct
    11161 gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt tagcacgtaa
    11221 atcgggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg cgccattatt
    11281 gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg caaaaacagg
    11341 ctgttgatac caatcggcaa ataccgccgt taacggttct gtacgaaaac gctgcgccca
    11401 ttggcgatcg gaacgctgac gttccgcgcg ttgttcagca ttttgcaatc caggatgccc
    11461 gccttcgaca acaactccac aaagccccgc cagctcctgg caagcagcca tcatcgccac
    11521 ccgtccgcca agcgagtacc ccaccagcca gaagttaagg atgttgtaac taaccagagt
    11581 tttacacaat aagccggtga catcatcaaa tccatcgacg ctgatcgtcg ctgaaccacc
    11641 gtgacctggg agatcaacat acaaccgcga gtagtcggca aacgcctcgc ccacttcttg
    11701 ccattcatgg caatcgccag aaaaaccgtg caaaaacacc aaccagggta aacctggttt
    11761 gccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag aagttgctgg
    11821 agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt ggtcggcgta
    11881 cgccaggcat cgactagtgt cgtttcaagt tcctgccagt tttgcggacg atgatatttc
    11941 agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag atagaaacgc
    12001 tcgcgctcgc ttttcggcgt tggcaacagc gaaaaaattt gcccgccgtt gttgttcacc
    12061 acaattaata ccagcggtgc ggaaacctgg cgcaataacg ccagcgcgtt gagatcgtaa
    12121 agtgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg ctgaacaccg
    12181 gcggcagtcg agagcagccc gtcgataccg ctggcaccac ggttgctgta caccgggtaa
    12241 cctgccggaa gttgcgaaag cgcatcaatc agacgcacca ccaggctgtt accgacaaac
    12301 aattgcccct gttcaggcaa gtagtcgctg atgcgatgcg ccagttgcgc ttcgccaaac
    12361 gcatcgcgac gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg gatttcaacg
    12421 caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat attggcaatt
    12481 aagcgacgtc cgcgatggtg tgccggatca agtcgccctt cgatgtcatc aacaatccag
    12541 tactcttccg gttcacagct tgcctgccat tgcaggagcc gtttgcccgt caggctgctt
    12601 cccagttgca ccacaatttg cgcctgttgc agttcgctgg tcgctttggc attgcctaac
    12661 cagagatcgg cacacggcag cggctgtccg gtttgcgaca gcacgtcgcc aatcagcggc
    12721 cagccaagag tttgcgccca cagtgcaact tttttgccct cttccgcact catgcgcccg
    12781 gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt ttcactttcc
    12841 cgatgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcgcc cagccgctgt
    12901 tgccagctaa tcccggtgtc gtccatttcg ccatacagcg gttcagcaaa cgggcagttg
    12961 atatggactc caccagcatg aagcgtaccg agagcgtggt cgatggtaga aaccagccaa
    13021 cgtgcgggga tatcctgggt cgggcgcggc agtgaaatac tgtgcgtggg gtgagaggcg
    13081 aacattcccg gctgacgaat cgcctgattc gcgccgcagt caatcagctc cggcgggcga
    13141 tcggcggtta acaggatcag tttttctccg gttaacccag cttcaatcag tgccggatag
    13201 agatttgcta ccgccgtgcc ggaggtcaca atcaccgcca caggctgctt gctgactttc
    13261 gccagcccca gtgccagatg ccccagtcca cgctcatcaa aatgggtatg atgaatgaat
    13321 gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagccagg ggcgatacag
    13381 atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca gcgtcggtta
    13441 aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta taaggagcta
    13501 caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt ccatttgtaa
    13561 taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct cggggtcgga
    13621 accacggaca atgcctgcgc cagcatatag ccgcacgata ttgccgctga tttttgctga
    13681 gcgcagggaa acgcagaatt cactttgctg taatgagaga tagcccgcag aaccggcgta
    13741 ccattctcga gtgaacggtt cgtgacgggc gataaactgt cgcgccagat cgcgcggtaa
    13801 gccagcaact gctgccgttg gctgcaactg atgtaaacag atcacatcat ccgctttgtt
    13861 gagtgaagtc cagatacagc ggcgaagatg ctgtacttta cgtagacgca gtacctgcgg
    13921 cggtaaaaca tccagcgttt gggtatcggc ctgcaatcgt tggcagatat cttccaccac
    13981 caacatgttc tcacgctggt ttttatcatc cgccatcagc cactcaccta actgctgcgc
    14041 ctgcttatca tcaggatgat ttgctaccgt tcccgccagc gcttcggtac gcagcacttt
    14101 gtcacgccgc cgccataacc gttccggtga agaaccaaga aaagcatttt cgccatcaaa
    14161 ggccatgtaa aaatggtagc aattcagatt gagccgacga ctggcagcca tcatcgctgc
    14221 cgcattgacc ggacttgcga aatgcaggtc agttgcccga gcaagcacaa ctttgtcgag
    14281 ctcgccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc ccgttttgtc
    14341 cggccagcgt tgttctcgcg tggtggttaa atgtaatcca ggcaggggct tgatactcac
    14401 cagtgtggcg ataaattctt ttgccttgat tgcatcgtgc tgaagggagc tttcgctgaa
    14461 tagcgtcagc cgcagcgtgg ctttaccgcc acaacgtcgc cattccaggc gtggtaaaag
    14521 taaattgccc tgcgacgggt caaacgcatt caacccccag atgcgtaagt cggcgtgttc
    14581 cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg taatcgcacc
    14641 caggacggca gcttcttcgt caccattacg ttgttgccag tagaattgcg gatacacctg
    14701 ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag gaatatcgat
    14761 aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta gattttccag
    14821 cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca ttatacgggg
    14881 tacgacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg ccagcaacag
    14941 tcccagaacc agcccaacgg ccgcacccac accaattcct tgccagggtt tttcgtggac
    15001 gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt ctgaagcctg
    15061 gctaacccgt tttttcacat catccagcgc tttttctgca cgcgctttca gctcaacata
    15121 tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt cactaagcag
    15181 cgtcaggtca tcatcaatac gtgtatcacc aaactgatta gacattctcg ttctccattt
    15241 gtgtaaaacc tgtcactaac tatagacaag ggttattgat tacgcctgaa ttacctcgcg
    15301 tgccatccca atgtgtggga taccatcttc ttcataaata tcggtcacag ggatgaaacc
    15361 aaattgccca taaaagtttt gtaaatgcgc ctgcgccccc agataaatcg gcttctccgg
    15421 ccagcggcga gtacacgatt ccagcgcctt actcatcaat tgctgaccga ttttttcacc
    15481 gcgtaaggtt tcgctgacaa tcacccgacc aatcacaacg ggttgcagtt cgtcgtcact
    15541 tttcagaatc ctcgcatacg ccaccagtgt cccgttatgc caaccgagaa tgtggcgatt
    15601 ttctccctcc agatcgtcac catcgatatc ctggtaagga cagttttgtt ccactacaaa
    15661 taccgcacaa cgcagttgta ataaggcata aagctgagaa acggataaat cagaatgatg
    15721 cagatcttgc cagtcgatca tggaaggctc ctttgtgctg tttgctttat actagccgga
    15781 atattttcag cagcgcgatg aactcatgga attaattttt ttaggtactt cagccggtgt
    15841 gccaacccgt acgcgtaatg tcacggcaat attgcttaac ctgcaacatc cgacccagag
    15901 cggactttgg ttatttgact gcggtgaagg cacccagcat cagctactgc ataccgcctt
    15961 taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc atctctttgg
    16021 tttacccggc ttactgtgca gtcgttctat gtcaggcatt atccaaccct taacgattta
    16081 tggtccgcac ggtatccgtg aatttgtgga gaccgcgctg cggattagcg gctcatggac
    16141 cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attttcgatg atggcctgcg
    16201 caaagtgacc gcttatccga tggaacaccc actggaatgt tatggttatc gtattgaaga
    16261 acatgataaa ccaggtgcat taaatgccca ggcattaaaa gctgctggcg tgccgcctgg
    16321 cccgctgttt caggaattaa aagcgggcaa aacaatcatg ctggatgatg gaaggcagat
    16381 taatggcgca gattacttag ccgttccagt gccgggtaaa gcgctcgcta ttttcggcga
    16441 taccggcccc tgcgatgccg cgctggagct ggctaaaggc gtcgacgtaa tggttcacga
    16501 agcgactctg gatatggcga tggaggccaa agccaacagt cgcggccata gttctacacg
    16561 ccaggcggcg gcactagccc gtgaggctgg agtcggtaag ctaatcatta cccacgtcag
    16621 ctcgcgctat gatgacaaag gttgtcagca cctgttacgt gaatgtcggt caattttccc
    16681 ggcgactgaa ctggcgaatg atttcgcggt gtttagcatt taagtcgaaa gcggtggtta
    16741 gttagcagat taatttaacg ataccaacgc atttatttga cattttttca tctgtttgcc
    16801 ggatacgttt gatgtcgata tcaggcaaac agaattatca tttttagttt tgtgaattgt
    16861 ccagatcttt cgccagtcca atcaggcgaa taaattccgc tctgtaaccc tgtggatctt
    16921 ccccttttgc cttctgagcc cactgtttga tctgctgcca ggaggtatta ttcaggtatt
    16981 cagaaccgcg taacttttgc ccatatgcag ccactgctgc gcgaaaacgc atatcttctg
    17041 aaggtgcctt tattgcgaat gccaacggga attcaactaa ctgactttct tttccctgtg
    17101 gggatttcca acgaatcttt aaccaggcaa gttcttttgt tttgtccgat ttcgctgatt
    17161 tgttatccgg ggcatagcgt aacttatcaa ttgatgcttt ttgccctttc agcgttaatt
    17221 caaataaaaa tgttatatgt ttacctgcgc caatatcacc cgcatcaacg ttgtcgttat
    17281 taaaatcctc tgccctaagt tggcgctttt cataacctat ctgacggtat tccgttaccc
    17341 acgcggggtt aaactcaatt tgcgctttga catcttttgc tacggtaatc aacgtctgcc
    17401 gcatttcact gttcaacact ttctgcgcct ctgagagggt atcaatgtag ctatagttgc
    17461 cgttaccaac atcggcaatt cttaccatca ttgcctcgtt ataattgcta tcccccacgc
    17521 caagcgtcga cagagtaaca ccggactcac gctgtttttt gaccattgat tcaatcgatt
    17581 ttggatcgtc aatgccaacg ttaaagtcac cgtcagtggc caataaaatg cgattgatac
    17641 cacctttaat aaaccccttc gctgcctgct gataagccat ctccagcccg gcaccgccat
    17701 tggtactgcc ttctacatcc agcgaatcaa ttgcagcatt aatttccgcc ttgtgactac
    17761 cggagataga aggcaacgca atacgagagt cgccagcgta ggtcacgatg gcaatgttat
    17821 cctgctcacg aagttctttg accaataatt tcaacgaaga ctggataagt ggcaaacgtt
    17881 catcagaaat cattgaacca gacgtgtcga taagaaagac cagattagaa gcgggtaact
    17941 cttcactttt gcgatctttc gccaggatat caactttcag caatgttcgc tgttcattcc
    18001 agggtgcagg tgccaattcg tagcgcatag cgaaaggtat tggcttagag gccggaatag
    18061 attgtttgtc tttgatatcc cagtcagacg ggaaataatt gactatctcc tccacccgca
    18121 cagcgtctgg cggaggtaac agcccttgat taaggaaacg ccgcacattc gcataactgc
    18181 cggtatcgac gtcaaggcta aacgtcgcca acgggttttg cgctacctgc ttaaccggat
    18241 tatcatcgaa ctgctggtag cgagcggttc ctggatttgc gatttgggag gctgcttttt
    18301 ctctggctgc atgttgatat tttggagccg cctgcaatcg ccccagcaaa gcctgtttgt
    18361 ctgaatattg ttgcacttct tgctgggcta aggctttcgc gtctgcagcg gctttggcgg
    18421 cgctttgctc agccactttt gttgccgctt gttgcgcggc aagcacttgc tggtcagtgg
    18481 gtgtagtaga ttgttgttgc tgacttttct tatcctcagg ttccggccca catcctgaca
    18541 aaatcaaact acttataagc aacatgatta tatttttatt tcgcatataa a
//
LOCUS       DADSZD010000087        18353 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.087, whole genome shotgun sequence.
ACCESSION   DADSZD010000087 DADSZD010000000
VERSION     DADSZD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.087"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(349..1053)
                     /locus_tag="J1Z28_002713"
     CDS             complement(349..1053)
                     /locus_tag="J1Z28_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBA7717535.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1190..1642)
                     /gene="moaE"
                     /locus_tag="J1Z28_002714"
     CDS             complement(1190..1642)
                     /gene="moaE"
                     /locus_tag="J1Z28_002714"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBA7717536.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1644..1889)
                     /gene="moaD"
                     /locus_tag="J1Z28_002715"
     CDS             complement(1644..1889)
                     /gene="moaD"
                     /locus_tag="J1Z28_002715"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBA7717537.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1882..2367)
                     /gene="moaC"
                     /locus_tag="J1Z28_002716"
     CDS             complement(1882..2367)
                     /gene="moaC"
                     /locus_tag="J1Z28_002716"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBA7717538.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2370..2882)
                     /gene="moaB"
                     /locus_tag="J1Z28_002717"
     CDS             complement(2370..2882)
                     /gene="moaB"
                     /locus_tag="J1Z28_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBA7717539.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLVFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2904..3893)
                     /gene="moaA"
                     /locus_tag="J1Z28_002718"
     CDS             complement(2904..3893)
                     /gene="moaA"
                     /locus_tag="J1Z28_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBA7717540.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3881..4025)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4290..5198
                     /gene="yvcK"
                     /locus_tag="J1Z28_002719"
     CDS             4290..5198
                     /gene="yvcK"
                     /locus_tag="J1Z28_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBA7717541.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     gene            complement(5236..7257)
                     /gene="uvrB"
                     /locus_tag="J1Z28_002720"
     CDS             complement(5236..7257)
                     /gene="uvrB"
                     /locus_tag="J1Z28_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBA7717542.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7836..8513)
                     /gene="bioD"
                     /locus_tag="J1Z28_002721"
     CDS             complement(7836..8513)
                     /gene="bioD"
                     /locus_tag="J1Z28_002721"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBA7717543.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            complement(8506..9261)
                     /gene="bioC"
                     /locus_tag="J1Z28_002722"
     CDS             complement(8506..9261)
                     /gene="bioC"
                     /locus_tag="J1Z28_002722"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBA7717544.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9248..10402)
                     /gene="bioF"
                     /locus_tag="J1Z28_002723"
     CDS             complement(9248..10402)
                     /gene="bioF"
                     /locus_tag="J1Z28_002723"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBA7717545.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLADSWSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10399..11439)
                     /gene="bioB"
                     /locus_tag="J1Z28_002724"
     CDS             complement(10399..11439)
                     /gene="bioB"
                     /locus_tag="J1Z28_002724"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBA7717546.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11526..12815
                     /gene="bioA"
                     /locus_tag="J1Z28_002725"
     CDS             11526..12815
                     /gene="bioA"
                     /locus_tag="J1Z28_002725"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBA7717547.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            complement(12926..14056)
                     /gene="tnpB"
                     /locus_tag="J1Z28_002726"
     CDS             complement(12926..14056)
                     /gene="tnpB"
                     /locus_tag="J1Z28_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HBA7717548.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            14165..14641
                     /locus_tag="J1Z28_002727"
     CDS             14165..14641
                     /locus_tag="J1Z28_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBA7717549.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            14793..16076
                     /locus_tag="J1Z28_002728"
     CDS             14793..16076
                     /locus_tag="J1Z28_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBA7717550.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(16217..>18353)
                     /locus_tag="J1Z28_002729"
     CDS             complement(16217..>18353)
                     /locus_tag="J1Z28_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBA7717551.1"
                     /translation="SSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLP
                     YVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKM
                     ILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQD
                     VALAIIGAVFKNGYVKNKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEV
                     HNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNL
                     TDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALR
                     GQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINN
                     GLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVP
                     EYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVT
                     TTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELAEV
                     FARIKQIAGQEHVDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATK
                     RYRSNVINWGMLPLQMAEVPNFDVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEI
                     TLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         4263 a         4988 c         4685 g         4417 t
ORIGIN      
        1 tgccgtaatt tcgagatact tagcgcccca ggccagccct gcggttgcca ccaaacccaa
       61 agcggtaatg agccatatgc tgtttaatat cctggcttcg tgctgaaaat ctaatttatt
      121 gctattttgc gagtatgatt ccattctttt agttgtcctt agaaaaaaca ttaatattca
      181 ccatcaataa atttaaaacc acgcatacaa aatacttttg catttatgga tacagaaaac
      241 gcttccgctc gtccataagg catattttat acctggtact tgttaagtgt cgtttttaat
      301 aagaaaaaaa tcccgacaca tggccgggat attgatgatg aaaaagaatt aacggcggtt
      361 gccgaagatc cgcagcaaca tcaggaacag gttgatgaag tccagataca aggttaacgc
      421 gccaagaatg gaatatttgc gcaggttcga cgtgtcgcga gtatcaatct gctcacccat
      481 atttttcagt ttctgggtgt catacgccgt caatccgaca aagacaatca cgccgatgta
      541 ggtgactgcc cacatcaatg cttcgctttt cagccagaag ttaaccaacg atgccagcac
      601 aatgccgatt aacgccataa acagcatatt gccaaagccg cttagatcgc gcttcgtggt
      661 gtaaccgtac aggctcattg cgccgaacat cccggcagta acgacgaaag tactggcgat
      721 agaagcagcg gtatagacaa tgaaaatact ggaaagcgta agacccgtca gcgccgaata
      781 aagcataaag agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg ctgataacac
      841 aataaccaat gctaattgcg cgatgatcag accgattaaa aagacacggt tagtaaacaa
      901 cagctccatc acggccgcgg aattagccgc ataccaggca acaaatgctg tcagcaacaa
      961 gccaacggtc atccagccat agacttgcgc catataagtt tgcaagccag cccggggttg
     1021 tacgattgaa tcagaacgtg ggaatctgtc catgactttc tcctgaagat ataaggaata
     1081 tcttaaggat actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac ggatgtactg
     1141 agcggtgaaa ttgccggacg cagcggtgcc ttatccggct aacaataaac taccagcgtt
     1201 ttgccgcctg ctgatcgctc tcccgagctt cgacccagcg gtcgccttcc ggcgtggctt
     1261 cgcgcttcca gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa
     1321 acgcactgct gcgatgcgca ctggtaacac cgacaaaaac aatttcatcg cccggccata
     1381 attcaccaat gcggtgaatt accgtgacgc gccccagcgg ccagcggtta cgcgcttcat
     1441 caacgatttc tgccagtgct ttttctgtca ttcccggata gtgttcgagg gttaatgctt
     1501 tgacgctgtc gcccaggtta tggttgcgca ccttaccggt aaaggtgact accgcaccgt
     1561 cttcgtcacg ctcagccagc cacgggtact cttctcctac gctgaacggc tgcgggccga
     1621 caacaatttt ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agccacttcg
     1681 tcgccgtcag tcagcgaatg gtcaaaactc accagcgtct ggttgacagc agccagcaat
     1741 ttgccatctt ccagcgccag cgcccagcga tcgctctgcg cagccagatg ctggcgtaac
     1801 gcttcaacgg ttgggaaatc cgcagccact tcggttgcat ctgttcccac caactcgcgc
     1861 acctgggcga aaaaaagaac gttaatcatc cgcttccacc ttaaagtcac ccgacttgcc
     1921 gccgctcttt gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca
     1981 catgtcataa atggtcagcg ccgccacgga ggccgcggtt aacgcttcca tttcgacacc
     2041 ggttttcccg gtcaggcggc ataaggtttc tatacgtacc cgattgtgct ccggctcggc
     2101 ctgtaaattg acttcaactt tgctgagcat cagcgggtga cacagcggga tcagatccca
     2161 ggtgcgtttt gccgcctgaa taccggcgat acgcgcagtg gcaaatacgt cgcctttgtg
     2221 gtggcgacca tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttcggc
     2281 gcgcgcttca cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc
     2341 ggcgttgata tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac
     2401 ggacgcgtac gggcatccag ctgcggcgca atgatatttt cccatgcggt acggcacgct
     2461 ttggtcgaac ccggcatggc gaaaaccagc gttttgttag cgacgcccgc taccgcacga
     2521 gattgcaacg tggaagtgcc aatctcttcg aacgacaaca tccggaacac ttcaccaaaa
     2581 ccttcaactt cacggtcgaa caacggcagc agcgcttcgg gagcctgatc gccttccgtc
     2641 aggccagtac cacccgtaat caacaccact tgtacatcgt cgctggcgat ccacgcagat
     2701 acctgagcgc gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg
     2761 cccgcttctt gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc ttcaccgcga
     2821 cgattagaaa ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc
     2881 atctgatctc tccttttgac gttttagcca ccaatgtacg ataagttttg cgtaataccg
     2941 gtgttatttt gatgcaggaa atgggtctgt tttttctccc gcagcgccgc tgaaatacgc
     3001 gcttccagcg cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct
     3061 tcaccaaaca ggcagagatg gagtttacca atggaggaaa cgcgcaggcg gttgcaagtg
     3121 gcgcagaagt ctttttcata cggcatgata aggccaatct ctccggcgta atccggatga
     3181 caaaagactt gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc
     3241 agtagctcgt cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcgctgccc
     3301 tcgcccgttt ccatcagttc gatgaaacgc agctggatgg gacgatgctg gatccagttc
     3361 agaaaggtgt cgagctggtg atgattaaca tcacgcatca gcacggtatt gactttgacc
     3421 ttctcaaaac cggcctcaaa tgcagcatcg attcctgcca tgacctggtt gaatttatcc
     3481 tgcccggtaa tggcatgaaa ctggcgagcg tccagactgt cgacgctgac gttgatgcca
     3541 gtaagtccag catcgcgcca gttcgccaca tcgcgctcca gacggtaacc attggtggtg
     3601 accgctatct ggcgaatagc gtcgttttct cgcacagcgg cgatgatatc ggtaaagtcg
     3661 cggcgtaaag atggctcacc acctgtcaga cggacttttt cggtgcccag actggcgaag
     3721 gcgcgcgtaa cccggcgaat ttcatcgacg gtcagaaagc ctttattggt gacgccgctc
     3781 ggcttgtaac catccggcag gcagtaggtg caacgaaagt tacacacatc agtaatcgac
     3841 aggcgcaagt agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc
     3901 tttccagata cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacgagc
     3961 attgtcacgg ccaaagcacc gtatcagttg acccaggtgc agaggctaga gtgtttagtg
     4021 gttaagccga tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc
     4081 atgtatatag cgtcatgatc gtgctatttc gctacagaat aagggaaaat ggcggtttta
     4141 ctttgatata catcatcagg gaggcatagc gtgcatcgtc ctttagaggt agaagaaaat
     4201 agtttgtatc ctcaatattg gcaggttaat tgctgtttcc tcgcaatttg cgctactgta
     4261 gcgcggataa tctgactcca ggaaactata tgcgcaatcg tacgctggct gatcttgatc
     4321 gtgtcgttgc tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt
     4381 tgggttctcg tttaacgggt atcgtcacca ccaccgataa cggtggctcg acggggcgta
     4441 ttcgccgctc agaaggcggc attgcctggg gcgatatgcg caactgcctc aaccagctga
     4501 taactgaacc cagcgtcgct tccgcaatgt ttgaatatcg tttcggcggt aatggcgaac
     4561 tttccgggca caaccttgga aacctgatgc tgaaggcact ggatcacctt agcgtgcgac
     4621 ctcttgaagc gatcaatctg attcgaaatc tgctgaaagt ggacgcacat ttgattccga
     4681 tgtcggagca tccggtggat ctgatggcga ttgacgatca ggggcatgaa gtttacggcg
     4741 aggtcaatat cgaccagtta actacgccga ttcaagagtt attgttaacg ccgaatgtac
     4801 ccgcaacgcg tgaggcggtt cacgctatca gtgaagcgga tctcatcatt attgggcctg
     4861 gcagttttta caccagcctg atgccaattc tgctgctgaa ggaaatcgca caggcattac
     4921 gccgcacgcc agcgccgatg gtctatatcg gcaatctggg gcgtgagttg agtttacctg
     4981 cggctaatct gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg
     5041 taattgatgc ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa gagcggattg
     5101 tgattcagga agtgctggag gccagcgata ttccgtatcg tcatgaccgc cagttgttgc
     5161 atagcgcgct ggaaaaggca ttacaggctt taggttaaca cgaagccgga agaatattcc
     5221 ggcttcgtca tcttgtcacg acgcggcaat aaacagctca cgcaactgat gcaactggtc
     5281 acgaatttgc gccgcttctt cgaactccag attctgcgcg tgttgcatca tcaacccttc
     5341 cagttcatgg atcttctgct gtaacgcttt aggcgacata tccatcggca cattatccgg
     5401 ctcaacaatc gggcgcgatt ttcctctgcc cttcgctttg gttttggcaa tgttctgccc
     5461 cagcgccagg atatcgacca ctttcttgtt caagccttgc ggcgtaatgc cgtgttcctc
     5521 gttgtacttc tgctgtttct cgcggcgacg ttcggtttcg ccaatcgctt tcgccattga
     5581 tggggtgatc ttatcgccgt agagaatcgc tttaccgtta acgttacgtg ccgcacgacc
     5641 aatggtctgg atcaacgaac gttcggaacg caggaagcct tctttgtcag cgtcgaggat
     5701 cgccaccagc gaaacttccg gcatatccag accttcgcgc agtaagttga tccctaccaa
     5761 tacgtcgaac tcacccagac gcaagtcgcg gataatctcc atacgttcga cggtgtcgat
     5821 atctgagtga agataacgca cgcgctcacc gtgttcttcg agatattcag tgagatcttc
     5881 cgccatccgc ttggtcagag ttgtaaccag tacgcgttcg ttaattgccg ctcgctgacg
     5941 aatctccgaa agaagatcat ccacctgtgt tgccactggc cgcacttcga taatcgggtc
     6001 gagtaagcct gttggacgca ccacctgatc caccacatcg ccaccggatt tttccagctc
     6061 gtaattaccc ggcgtcgccg aaacatagat ggtttgcggc gctaatgctt cgaactcttc
     6121 aaatttcagc ggacggttat ccagcgctga tggcaggcgg aagccgtact ccaccagtgt
     6181 ctctttacgc gcccggtcac cgcgatacat gccgccaatt tgtggaatgg tgacgtgaga
     6241 ttcatcgacc accagcagcc catcagcagg caggtaatca aacagcgtcg gcggtggctc
     6301 acccggtcca cgaccggaga ggaagcgcga gtagttctca atccccgaac agtagcctag
     6361 ctcgttcatc atctccagat caaactgggt acgctgggtc agccgctgct cttccagcag
     6421 tttgttgttt tccaatagca ctttacgtct ggcagccagc tcttctttga tctcctccat
     6481 cgcctggacg atgcgctcgc gtggcgtgac gtagtgcgtt ttcgggtaga tggtaaaacg
     6541 tggaatagtg gaaacaatct gcccggtcag cgggtcaaat aacgacaatc gttccacctc
     6601 ctcgtcaaac agctccacgc gaagtgcaat gtcatccgat tctgccggga agatatcaat
     6661 cacctcgcca cgaacacgga aagtaccacg ctggaatgct tgatcattac gagcatattg
     6721 cagctccgcc agtcggcgca ggatcgcgcg ctgatcgata atcataccga ccgtgagatg
     6781 gagcatcatc ttgagatata aatcaggatc gcccagacca taaatcgcgg aaacagacgc
     6841 caccacaacc acatcacgcc gctccagcat cgctttggtg gcggacaaac gcatctgttc
     6901 gatatgttcg tttaccgagg catctttctc aataaaggtg tcggaactcg gtacataggc
     6961 ctccggctga tagtagtcgt agtaggagac gaaatattcc accgcgtttt ccgggaagaa
     7021 ttctttcatt tcgccataca gctgggccgc cagcgttttg ttgggcgcaa gtaccatggt
     7081 tgggcgctga aggtcagcaa tgacattggc aatggtgaag gttttcccgg agccagtcac
     7141 gccaagtaac gtctggtgcg ccaggccatc ttccagcccc tcttcgagac gtcgaatcgc
     7201 ctccggctga tcgccagaag gtttaaaagc ggaattcagt ttgaacggtt tactcatgag
     7261 tcgctacctg aaggagttgg gcgggcaggt atgtaatttt actcgtcgta cttaattatg
     7321 ccaacaaatt atactggata aaaaaacagt tcatcaccat aatatttctg ttacagcgta
     7381 aactccgctg tcaatcatga acaagattta ctctgcggcg agataaaact ccggccttac
     7441 cgggttatcc ccaaagcaac ggctttttta acatttgtca agatgattga tgacagtttt
     7501 atggcaagag atgcctgttc agtgactgcc attgcaatta tctaacctgc taaaaaataa
     7561 aagatatttc tttgagccgt ctttaacgcc aattcgcgtg caagccgcgt attctctcgc
     7621 ttgcctcgtg ttttctaact ctaatacaca tggttatcca caggaatagt ggataactgt
     7681 ctgcagcccc tatcccccgc cgcctgggca attcccacat tcaccgcatg atgcggaggt
     7741 aaaatttttt tcgtgatttt tttagaattt aattggttaa attgcagtca atcggagaca
     7801 cgatctcgct cgcaatttaa ccaattacag gatgactaca ttaaggcaag attgatgtac
     7861 tttccggttg ccgcattttc tggattttct gcaagccagg gaatttcgcc cagcagcggc
     7921 gcgggaatca tgcgggtgag cgtggtcata tattcagcgt gacgttttcc cggaggcgtg
     7981 acatcgttcg ccacccaccc cgccagagtc agtccggcgt gtagtattgc ctgcgcagtc
     8041 aacatcgcgt gattaataca gccgagtttc acaccaacta ccagtatcac cggcagttgt
     8101 tcctgtgtta cccaatccgc aaaagtgaaa gtgtcagaaa gcggcgtaaa ccagccgcca
     8161 gcaccttcca ctaacaccca gtcagcctgt tgttcaagcg cgcgtaatcc ggcgctcatt
     8221 accaatgatt ctatcggcct gccctcttgc gcgctgatga tatgcggcga ggtgggttct
     8281 gcgaaggtgt aaggatttac cgttgcgtaa tccagctgca ggctactgtt gcgctgtaac
     8341 gccagcgcgt cactattacg taagcctttc ggggtctttt cgctgccaga ggccaccggt
     8401 ttgtaacctg ccgtccggta gcctgctgcc tttgcggctt gtaaaagcgc gcagctggcg
     8461 acagttttcc ccacttcggt atccgttccg gtgacaaaat aacatttact cacgagcaat
     8521 cactcccaaa aaaagatgat acgtcagagg atatcgcccc tgttgttgcg gccaggccag
     8581 ttgcaatcgc tgcaactgcg aacgcgttaa tattcgcggg tcacgccctt catgaagatg
     8641 cgtggcaccg atgcctttca gcgaacgcat ggcactgagc gcatcatcaa accacagcgt
     8701 gatgggctga atataatgtt gataatgcac ggcgtttagc gactgttcga tttcatctgg
     8761 cggtaaaaag cgattagcat gcggacgctc gtccaccgcc tgccacgcct gatgcagttc
     8821 gggtaacgat ccctgcacca gcgtggtaaa cgcgaccacg cctccggggc gcaccacccg
     8881 atacagctcg cggagtgccg tggataaatt accgcaccac tgcaccgcga gattgctcca
     8941 tgcaagatcg aacgtcgcag tcgctaacgg caaggattcg atatctcccg ccagataatg
     9001 gtctgcggca tccttctggc gtgcctgaac aagcattggc ggagagagat ctaacgcagt
     9061 cacctgcgcg tgacgctctc gccagcggcg gctcatccag cctggtccac aacccgcatc
     9121 cagtacgtgg gtgtatttac gctgtgggag cattgccagt aaggcgtcag cactctggcg
     9181 ctgtagatcc gcgtgttgct catagtgtgc ggctgcccgt ccaaatgccg cagcaatggc
     9241 ttgtttatta accgttgcca tgcagcacct ccagcagacg gtcgatatcc tgcatttcat
     9301 gcgctgcggt tagcgttaag cgcagtcgcg cagtaccagc gggtacggtt ggcgggcgaa
     9361 tcgccgtgac ccagcagcct tgctggcgca gtttttctgc cagttgtaac gcacggctgt
     9421 tatcaccgac gatcaatggc tggatggcgc tccatgagtc agcaagcgta aacggcaaat
     9481 cctgcactcc ggcacgaaaa cgagtaatga gtgccgccag tttttcgcgc cgtgcatccc
     9541 cctcatcact gcgaatgacc gccagcgacg cacgtaatgc ctgcgcctga gcgggcggca
     9601 tactggtgct gtagataaga tggcgagcga attgcagcag ataatccgcc accgtattgg
     9661 agcaaagcac cgctgccccg ctgacgccaa atcctttgcc aaacgtcacc accagcaatt
     9721 ctggttttac cttttgcagc cagcagctgc cacgcccctg ttccccgata acgcccgtgc
     9781 cgtgggcgtc atcgaccatc aaccagccat cgtgctgttg cgttacctgc tggatttcct
     9841 ccagtggcgc actatcgccg tccatgctga acacaccttc tgtcactacc agttgctgcc
     9901 ccggacaggg ggaagcaagc agtcgcgcca gatgagtgac atcgttatga gcaaaacggc
     9961 gaagcggcga cgggcttaaa cttgcagcct ccagcaatga ggcatggcta agccggtcgg
    10021 caacaatacg gtcctctttc gccatcatcg cggcaatgac agcctgatta gcggcaaaac
    10081 cagaaataaa cagcagtgcc cgcgaatagc ccagccattc ggccagttct tcttccagca
    10141 cctggtgcgc cacgctataa ccgctgacgt gaccggaacc gccgctaccg acgccaaatt
    10201 gttccgcccc ctgctgccag gcgcggataa tttgcggatg atggcttaaa cctaaataat
    10261 cgttactgga aaagttcaga tactggcaat catctgccac cagccagcgt ccggctcctt
    10321 gtgccaccgg ataacggcga cgcaaggcat cagcaacacg ccgcgcatca agcgccgcgt
    10381 cgattttctc ctgccagatc atagtgctgc cgcgttgtaa tattcgtcag tgtccggggt
    10441 catcagcgcc tgctccagac gctgctgttg ttcgttatcg cccgccagca cggcagtttg
    10501 ctgcggattt agccccagtt tgcggaacag ttgcaggtct ttatcttctt ccggattcgg
    10561 cgtagtcagc agtttgcaac cgtagaaaat cgagtttgcg ccagccataa agcacatcgc
    10621 ctgagtctgt tcgttcatct gctcgcgtcc ggcagaaagg cgtacgtaag aggtcggcat
    10681 catgatccgc gctacggcaa tggtacggat aaaatcaaag gcatcgacat cgtcgttatc
    10741 cgccagcggc gtgcctttca ccttcaccag catgttgatt ggcacgcttt ccggcggcgt
    10801 cggcaagttt gccagttgca gcaataatcc ggcgcgatct ttcaccgttt cgcctaagcc
    10861 cacaatgcca cctgaacaga ctttaatccc agcttctcgc actttttcca gcgtatcgag
    10921 acgttcctga taggtgcggg tggtgatgat attgccgtaa aactccggcg aggtgtcgag
    10981 gttgtggtta tagtaatcca gcccggcatt cgccaggcgc tgcgcttgag attcactcaa
    11041 cgtgcccagc gtcatacacg cctccagacc catcgctttt accccctgca ccatttgttc
    11101 caggtacggc atatcgcgtt cgtggggatt cttccacgcc gcgcccatac agaaacgcgt
    11161 cgatcccgcc gctttcgctt tgcgcgccga ctccagcacc tgttcaactt ccatcaaccg
    11221 ctcggcttcc agcccggttt tgtagcgcga gctttgcggg caatatttgc aatcttccgg
    11281 acaagctccg gtcttaatcg acagcaaagt gctgacctgc acctgacgag gatcgaaatg
    11341 ctgacgatgc acctgctgcg cttcaaacag cagatccagc aacggttttt caaataattc
    11401 tgtgacttgc gacaatgtcc agcgtgggcg gtgagccatg gggcttctcc aaaacgtatt
    11461 ttttgttgtt aattcggtgt agactcgtaa acctaaatct tttcaatttg gtttacaagt
    11521 cgattatgac aacggacgat cttgcctttg accaacgcca tatctggcac ccatacacat
    11581 ccatgacctc cccgctgccg gtttatccgg tggcaagcgc cgaaggttgc gagctaattt
    11641 tgtctgacgg cagacgcctg gttgacggta tgtcgtcctg gtgggcggcg atccacggtt
    11701 acaatcaccc gcagcttaat gcggcgatga agtcgcaaat tgatgctatg tcgcatgtga
    11761 tgtttggcgg tatcacccat gcgcccgcca ttgagctgtg ccgcaaattg gtagcaatga
    11821 cgccgcaacc gctggagtgc gtttttctcg cggactccgg ctccgtagcg gtagaagtgg
    11881 cgatgaaaat ggcgttgcag tactggcagg ccaaaggcga agcgcgccag cgttttctaa
    11941 ccttccgcaa tggttatcat ggcgatacct ttggcgcgat gtcggtgtgc gatccggaca
    12001 actcaatgca cagcctgtgg aaaggctatc tgccagaaaa cctgtttgct cccgccccgc
    12061 aaagccgcat ggatggcgaa tgggatgagc gcgatatggt gggctttgcc cgcctgatgg
    12121 cagcgcatcg tcatgaaatc gcggcggtga tcattgaacc gattgtccag ggcgcaggcg
    12181 ggatgcgcat gtaccatccg gaatggttaa aacgaatccg caaaatgtgc gatcgcgaag
    12241 gtatcttgct gattgccgac gagatcgcta ccggatttgg tcgtaccggc aaactgtttg
    12301 cctgtgaaca tgcagaaatc gcgccggata ttttgtgtct cggtaaagcc ttaaccggcg
    12361 gcacaatgac cctttccgcc acgctcacca cgcgcgaggt tgcggaaacc atcagtaacg
    12421 gcgaagccgg ctgctttatg catgggccaa cttttatggg caatccgctg gcctgcgcag
    12481 cagcaaacgc cagcctggcg attctcgaat ctggcgactg gcagcatcag gtggcggcta
    12541 ttgaagcgca gctacgcgag caacttgccc ccgcctgtga cgccgaaatg gttgccgatg
    12601 tgcgcgtact gggggcaatc ggcgtggtcg aaaccactcg tccggtgaat atggcagcgc
    12661 tgcaaaaatt ctttgtcgaa cagggtgtct ggatccggcc ttttggcaaa ctgatttacc
    12721 tgatgccgcc ctatattatt ctcccgcaac agttgcagcg tctgaccgca gcggttaacc
    12781 gtgcggtgca ggatgaaaca tttttttgcc aataacggga agtcagcgtg agggtttctg
    12841 gctacacttt ctgcaaacaa gaaaggaggg ctcttgacgg ctccccgccc tgaatgacgg
    12901 ggattcccac ttcaacgagc cgaacctaag ccgcgtgctg cgattcaggg tttacaggct
    12961 ctccgtgggc taacactgcc agcccggcag ctttaatatt acgtgccgcg ttaatgtcgc
    13021 gatcatggtc tgcgccgcat tcagggcagt gccatttacg aacattaaga ggcatttttt
    13081 gcatggtgaa accgcagcaa ctacagcatt ttgaggacgg taaatactgg tcaatggcga
    13141 ccactgaccg cccggcccat ttgcctttgt actggagctg gcgaacaagt tcgctccagc
    13201 ctgcgtcagc tattgcttta gacagcttcg ggttgcggat catgtttttc accttgaggg
    13261 attcgacgca aacaacttgg ttttcgttaa tcagtttgcg ggacaacttg tgcagattgt
    13321 ccatccggca atcggcgatt ttcgcgtgga gtcgggcgac ctttaagcgg gctttaatac
    13381 ggtttcttga gcctttttgc ttcctgctta aacgtcgctg tagcagcgtt aatcgcttcg
    13441 catatttagc ggtgtggcgg ggattgtcgg ttttgaatcc ggtatcggtg acgaataaat
    13501 cttttaagcc cacatcaatg ccgaccgttt tagcggtaac aggcattgat acaggttcaa
    13561 actcacacag gcaggaaaca aagtacctgc cagcgctatc tctggaaatg gtaacggttg
    13621 acggcgcaga tggtaattct cgactccagc gaacatccag cggcgacttg ctctttgcta
    13681 tatacaactc gccgtcacgg tgtttaaacg cgctggcagt gaactcagcc acctgtttgt
    13741 gccgtttgct tttgaaagcc ggatatgcag ctcgtccggc aaagaagtta gcaaaggcgg
    13801 cttgttggtg gcgcaacgac tgctggaggg gaacgcagga aacatcattc agccatatgt
    13861 attcaggctc ttttttgagc gccgtaaggc gagcgttggc ctgtagataa ccgatctttt
    13921 cttttcgctc gtagtacgca tcggtacgcc aacgaaggat ggaattgtag acgaagcgca
    13981 cacagccaaa cgtctgagct aaaagctcag cctgctcagt tgtcgggtaa aaccggtatt
    14041 tatatgcgcg tttcatgtgt tcacatacta aagaggaaaa tgtgattatg caaagtacag
    14101 ttagtcggaa aaccgcctcc tttcctcccc ggtctgaagg ccgaggtttc ccggaggcat
    14161 tctaatgaaa ctcatcagta acgatctgcg cgatggcgat aagctgccgc atcgtcatgt
    14221 ctttaacggc atgggttacg atggcgataa tatttcgccg catctggcgt gggatgatgt
    14281 tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac ccggacgcgc caaccggctc
    14341 cggctggtgg cactgggtag ttgttaattt acccgctgat acccgcgtat taccgcaagg
    14401 gtttggctct ggtctggtag ctatgccaga cggcgttttg cagacgcgta ccgactttgg
    14461 taaaaccggg tacgatggcg cagcgccgcc gaaaggcgaa acccatcgct acatttttac
    14521 cgttcacgcg ctggatgtag aacgtattga tgtcgatgaa ggtgccagcg gcgcgatggt
    14581 cgggtttaac gttcattttc actctctggc gagtgcctcg attactgcga tgtttagtta
    14641 atcactctgc cagatggcgc aatgccatct ggtatcactt aaaggtatta aaaacaactt
    14701 tttgtctttt taccttcccg tttcgctcaa gttagtataa aaaagcaggc ttcaacggat
    14761 tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt tcccgtctgg
    14821 cgctggcatt ggcttttggc gtgacgctga ccgcctgtag ctcaacaccg cccgatcaac
    14881 gtccttctga tcaaaccgcg cctggtacct cttcgcgccc gattctgtcg gcaaaagaag
    14941 cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgcg gcagcgtgga
    15001 atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg gcgggtactc
    15061 aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc aagcgtacca
    15121 acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggtg tatgtccctg
    15181 ccgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat gtgaagattg
    15241 ggctttccct tgatggtggc atgagccctg ccgactggcg tcacgacgtc aacccgcgcg
    15301 gcaaatatat gccaggtaaa ccggcgtggt atatgtacga tagctgccag agtaaacgca
    15361 gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat ggcctgcaac
    15421 tgcaaaacct gaccatcgaa aacacgctgg gcgatagcgt agatgcgggt aaccatccgg
    15481 cggtggcact gcgtactgat ggcgacaaag tgcagatcaa taacgtcaac attctcggtc
    15541 gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa acgaatcgtc
    15601 agccgcgtac gctggtgacc aacagctata ttgaagggga tgtggatatc gtttctggtc
    15661 gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcccgt acccagcaag
    15721 aagcgtatgt gtttgcaccg gctacgctgt ccaacattta ctacggtttc ctcgccgtaa
    15781 acagccgttt caatgcttcc ggtgatggcg tggcgcaact gggccgctcg ctggatgttg
    15841 atgccaatac caacggtcag gtagtgatcc gtgatagcgc catcaacgaa ggttttaaca
    15901 cagccaaacc gtgggctgat gcggtgattt ccaatcgtcc attcgcgggt aacaccggca
    15961 gcgttgatga taacgacgaa gtacagcgca atctgaatga cactaactac aaccgcatgt
    16021 gggaatacaa taaccgcggc gtgggtagca aagtggttgc agaggcgaag aagtaggagc
    16081 tgttaactat gtgccggatg cggcgtaaac gccttatccg gcctacggtt cgatgcgatt
    16141 tgtcggtcgg ataagatgcg ccagcatcgc atccgacaat aagtgccgga tgcgacctac
    16201 attcacatgg cgctttttac atctgacggt ttttattgaa gttaatcaaa ctacccgcct
    16261 tgataatctc gcgctcttca gcagtcagac tttccatata gagcgtaatc tccgttaccg
    16321 gtgcatcttc atggatcaca taacctttaa acgtcgttcc aggattatcc agcgccgctt
    16381 taatgccagg gatataaatg taatcaccta cgtcaaagtt cggtacttcc gccatctgca
    16441 gcggtaacat cccccagttg atgacgttag aacgatagcg tttagtcgcg tactcctcgg
    16501 caatattcgc cagaccgcca atcacacgct gacaactcgc cgcctgttca cgcgcagaac
    16561 catcgcctgg tttcaccgca taaaccatgc tgccaatttc agtttgcagc ggatcaacat
    16621 gttcctgacc agcaatctgc ttaatgcgcg caaacacctc tgccagctca ctgacatttc
    16681 ccgccagacg ctgattttcc agctcggcag tcgctttact tctgccaaca taacctggat
    16741 cgcggcgtga cagagtaaac tccgccagac caatcggatt tgaacgatat gaagaggttt
    16801 caccggaagg aatcagttcg tcggtggtag tcacttcgtc gaggatcttc gagcacactt
    16861 tgaggacgat attgtcagtc agcgcaccca attctggcca gtctttaatg ttcggtccgt
    16921 aaatcagcgg ttgctgggtc gcccctttca caaagccctg ataaacacgg tttttatacg
    16981 gcgttacatc gaaggcgtac tccggcacgt tgtcccagca atcgagttcg ctggcagagg
    17041 ttaaatagcc accgtttgcc gcagtcgcag cgatagaacg agcgtccatc aacgccaccg
    17101 ctgacatctg cccattagct ggcttagagc cttcgcggtt cgggaagtta cgcgtggtgt
    17161 ggcgaatact caaaccgttg ttgattggcg tatcgcccgc accaaagcat gggccgcaga
    17221 acgcggttct gatgattgcg cctgcgccaa tcaaatctgc gaccacacct tttttggcga
    17281 gatccataaa caccggctgt gatgacgggt aaaccgccaa cgagaaggta tcgttgccgc
    17341 aggattgacc gcgcagcgca ttcgccgccg cgatgacgtt ttcgtagtta ccgccagaac
    17401 agcccgcgat aatcccctgc tgcactttca ggcgaccatt ttccacttta tccagcagcg
    17461 agagtttggc tttaccgtgc gccacgcgtt cggactcaat ttcaatctca cgcagaatgt
    17521 cggtcaggtt ctggttcagc gtgtcgattt catacacgtt gctcgggtgg aatggcagcg
    17581 caatcattgg tttgatggcg cttaaatcaa cgctgatgca gccatcgtag tacgccattg
    17641 gttgagggtt aagttggcag taatcctggc cgcgaccgtg cagcgccagc cagttatgga
    17701 cttcttcatc ggtttgccag acagaactta aacaggtcgt ttcagtggtc atcacgtcaa
    17761 cgctgttacg gaaatcggta gagagcgctg caacaccggg acctacgaac tccatcactt
    17821 tgtttttgac gtaaccgttt ttgaacacgg caccgatgat agccagcgcc acatcctgcg
    17881 gccccacata cggcgcaggt tttccggtca gatgcaccgc aaccacgccc ggatagtcga
    17941 tatcccaggt gtcattaagc agctgtttta ccaactcgcc gccgccctcg ccaaccgcca
    18001 ttgtccctaa cgcaccgtaa cgggtatggc tgtctgaccc gaggatcatt ttgccgccgc
    18061 ccgccatcat ttcacgcata tattgatgga tgaccgcaat atgcggtggc acaaaaatac
    18121 cgccataacg ctgggccgcc gataaaccaa aaacatggtc atcaccgtta atggtgccgc
    18181 ccacagcaca gagtgagtta tggcagttgg tcagcacata cggcagcggg aaacgttcca
    18241 taccggacgc tttagcggtc tgtacaatac caacaaaggt aatatcgtga gaggctaatg
    18301 aatcaaactt aattttaagt ttatccatat ttccggacgt attatgagaa gag
//
LOCUS       DADSZD010000088        18326 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.088, whole genome shotgun sequence.
ACCESSION   DADSZD010000088 DADSZD010000000
VERSION     DADSZD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.088"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..566)
                     /gene="rsmG"
                     /locus_tag="J1Z28_002730"
     CDS             complement(<1..566)
                     /gene="rsmG"
                     /locus_tag="J1Z28_002730"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBA7717552.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKL"
     gene            complement(630..2519)
                     /gene="mnmG"
                     /locus_tag="J1Z28_002731"
     CDS             complement(630..2519)
                     /gene="mnmG"
                     /locus_tag="J1Z28_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBA7717553.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2898..3341)
                     /gene="mioC"
                     /locus_tag="J1Z28_002732"
     CDS             complement(2898..3341)
                     /gene="mioC"
                     /locus_tag="J1Z28_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBA7717554.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEETGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3431..3889)
                     /gene="asnC"
                     /locus_tag="J1Z28_002733"
     CDS             complement(3431..3889)
                     /gene="asnC"
                     /locus_tag="J1Z28_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBA7717555.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4041..5033
                     /gene="asnA"
                     /locus_tag="J1Z28_002734"
     CDS             4041..5033
                     /gene="asnA"
                     /locus_tag="J1Z28_002734"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBA7717556.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5038..6489)
                     /gene="viaA"
                     /locus_tag="J1Z28_002735"
     CDS             complement(5038..6489)
                     /gene="viaA"
                     /locus_tag="J1Z28_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBA7717557.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRMKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6483..7979)
                     /gene="ravA"
                     /locus_tag="J1Z28_002736"
     CDS             complement(6483..7979)
                     /gene="ravA"
                     /locus_tag="J1Z28_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBA7717558.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8202..10070
                     /gene="kup"
                     /locus_tag="J1Z28_002737"
     CDS             8202..10070
                     /gene="kup"
                     /locus_tag="J1Z28_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBA7717559.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10237..10656
                     /gene="rbsD"
                     /locus_tag="J1Z28_002738"
     CDS             10237..10656
                     /gene="rbsD"
                     /locus_tag="J1Z28_002738"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBA7717560.1"
                     /translation="MKKGTVLNSDISSVISLLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10664..12169
                     /gene="rbsA"
                     /locus_tag="J1Z28_002739"
     CDS             10664..12169
                     /gene="rbsA"
                     /locus_tag="J1Z28_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBA7717561.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKLQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12174..13139
                     /gene="rbsC"
                     /locus_tag="J1Z28_002740"
     CDS             12174..13139
                     /gene="rbsC"
                     /locus_tag="J1Z28_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBA7717562.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13164..14054
                     /gene="rbsB"
                     /locus_tag="J1Z28_002741"
     CDS             13164..14054
                     /gene="rbsB"
                     /locus_tag="J1Z28_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HBA7717563.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14180..15109
                     /gene="rbsK"
                     /locus_tag="J1Z28_002742"
     CDS             14180..15109
                     /gene="rbsK"
                     /locus_tag="J1Z28_002742"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA7717564.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15113..16105
                     /gene="rbsR"
                     /locus_tag="J1Z28_002743"
     CDS             15113..16105
                     /gene="rbsR"
                     /locus_tag="J1Z28_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBA7717565.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16071..17498)
                     /locus_tag="J1Z28_002744"
     CDS             complement(16071..17498)
                     /locus_tag="J1Z28_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBA7717566.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYAHKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNHVPSESE"
     gene            complement(17521..18213)
                     /locus_tag="J1Z28_002745"
     CDS             complement(17521..18213)
                     /locus_tag="J1Z28_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBA7717567.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4390 a         4549 c         4801 g         4586 t
ORIGIN      
        1 tgaagtttaa ccactgattc gacctgatat tcttcgggca acaaagcaat ttcatcttcc
       61 ggcatttgcc ctttcagcgc gtagaaacgg ccttgctcac caggaagatg gtggcaccag
      121 ctcaccatat cgttcagaga ggcaaaagcg cggctaatta cgccatcaaa tggcggctct
      181 gacggaaact cttctaccct gctctgtact ggctcaatat tctccagttt aagctcatgt
      241 tgcacctgac gaaggaaacg cacgcgttta ccaaggctat ccaacaacgt gaaatgagct
      301 tcaggacgca cgatagagag tggaatgcca ggcagtcccg gtccggtgcc gacatcgata
      361 aaccgttcac cttgcagata cggtgccacc acaatgctat cgagaatatg gcgtaccagc
      421 atctcattag gatcgcggac cgaagtcagg ttgtacgctt tattccattt atgcagcata
      481 ttcacgtagg caataagctg gtttttctgg tgatcggtaa gcgaaatacc tgcgtctttc
      541 agcagtaagg agagtttgtt gagcacggtg attacctgtt cttgatgcgt tgcctggtaa
      601 gcgggtgctt accaggcatt tttaatgcgt tatgcgctac gacgcagcat accctgtttt
      661 ttcagccaca ccagcagaat ggagatagcc gcaggcgtga caccagaaat acgcgaagcc
      721 tggccaatag aggccggttt gtgatcgtta agtttggcga tcacttcgtt agaaagaccg
      781 gatacctggc ggtaatccag cgtcgctggt agcagggtat tctcgttacg cagctgcttt
      841 tcgatctcat cttgctggcg cgcgatataa ccttcgtatt taacctgaat ctcaacctgt
      901 tccgccgcct gttcgtctgt caacgcaggg gcaaacggcg tcagcgtggt taatttttca
      961 taagtcattt caggacgacg cagcagatct tcaccactgg cttcacggga aagcggcgct
     1021 gtcaggtgag cattcacttc ggctgcagct tccgccgacg gggttaccca ggtcgatttc
     1081 agacgctgac gctcacgctc gatattctca agtttctcgt taaagcgcgc ccaacgttcg
     1141 tcatccacca ggcccagttc gcgaccgatt tcagtcaaac gcagatcagc attatcttcg
     1201 cgcagcatca gacgatattc tgcgcgcgag gtaaacatac gatacggttc tttggttcct
     1261 aaagtgcaca ggtcatcaac cagtacgccg agatatgcct gagaacgcgc cggagcccaa
     1321 ccttctttgt cagcagacag acgggcagcg ttaagaccag ccagcaaacc ttgcgcagcg
     1381 gcttcttcgt aaccggtagt gccgttaatc tgaccagcaa agaacagccc ctggataaac
     1441 ttgctctcca gcgtcggttt caggtcgcgt ggatcgaaga agtcatactc aatggcataa
     1501 cccggacgca cgatcttcgc gttttccatc ccctgcatgg agcggacgat ttgcatctgc
     1561 acatcgaacg gcaggctggt ggagataccg ttcggataaa tttcgttaga ggtcagccct
     1621 tccggttcaa ggaagatctg atgctgattt ctgtcggcaa agcgcatgac tttgtcttcg
     1681 atcgacgggc agtagcgtgg gccgacacct tcgatcaccc ctgcgtacat tgggctacga
     1741 tcgaggttac tgcggatcac atcatgggtt ttctcgttgg tatgagtgat ataacacggc
     1801 acctgctggg gatgctggga cgcattgccc ataaacgaga atactggcat tgggttatcg
     1861 ccatgctgtt gcgccagtac gctaaagtcg atggttcgag catcaatacg cggtggtgtc
     1921 ccggttttca gacgaccaac gcgcagcggc agttcacgca aacggcgaga aagcggaatg
     1981 gacggcggat caccagcacg gccaccgctg taattatcca gaccgatatg aattttaccg
     2041 tcgaggaacg tcccaacggt gagcacgaca gctttggcac ggaacttcag tcccatttgg
     2101 gtaacggcac cgaccacgcg atcgttttcg acaataagat cttcaaccgc ctgctggaag
     2161 atcatcaggt tcggttggtt ctccagcgcc gtacgtaccg cctgacggta gagcacacga
     2221 tctgcctgag ctcgggtagc gcgaaccgcc ggtcccttgc ttgcgtttag tatcctaaac
     2281 tggatacccg cctgatcgat cgctttcgcc atcagaccgc cgagtgcatc cacttctttt
     2341 accagatgtc ccttcccaat accgccgatc gccgggttgc agctcatctg ccccagagtg
     2401 tcgatattgt gtgtcaaaag cagagtctgt tgacccatac gcgccgcggc catcgcggcc
     2461 tcggtgcctg catgaccccc gccaatgatg atgacgtcaa aaggatccgg ataaaacatg
     2521 gtgattgcct cgcataacgc ggtatgaaaa tggattgaag cccgggccgt ggattctact
     2581 caactttgtc ggcttgagaa agacctggga tcctgggtat taaaaagaag atctatttat
     2641 ttagagatct gttctattgt gatctcttat taggatcgca ctgccctgtg gataacaagg
     2701 atccggcttt taagatcaac aacctggaaa ggatcattaa ctgtgaatga tcggtgatcc
     2761 tggaccgtat aagctgggat cagaatgagg ggttatacac aactcaaaaa ctgaacaacc
     2821 attgttcttt ggataactac cgtttgatcc aagcttcctg acagagttat ccacagtaga
     2881 tcgcacgatc tgtatactta tttgagtaaa ttaatccacg atcccagcca ttcttctgcc
     2941 ggatcttccg gaatgtcgtg atcaagaatg ttgatcttca gtgtttcgcc tgtctgtttt
     3001 gcaccggaat ttttgagttc tgcctcgagt ttatcgatag ccccacaaaa ggtgtcatat
     3061 tcacgactac caataccgat tgcgccaaag cggaccgcag aaagatcggg cttctgttcc
     3121 tgcaatgctt catagaaagg agaaaggttg tccggaatat ctccggcacc gtgggttgag
     3181 ctgataacca gccagatccc cgaggcagat aaatcttcca acagcggacc gtgcagcgtt
     3241 tcggtggtaa aacccgtctc ttccagcttt tcagccaggt gttctgctac atattcggca
     3301 ccgccgaggg tgctgccgct gataagagtg atatctgcca taaaccgcca cctttattaa
     3361 gagtagcgta ttgtacgctg tgaacgcgtt gggatctacc tgtggaaaag tatgggatta
     3421 aaaaagccga tcagggctta atggtacgca tgatcgggtt ctgcaggacg atcagtgtct
     3481 cggtggactg aatttcatca attgtttgga tcttgttgat aagtacatgc tggagagcat
     3541 cgatcgaacg gcacatcact tttataaaga tgctgtagtg gccggttgtg taataggctt
     3601 cagtgacttc atcgaggctt tccagctttg ccagcgcgga aggatagtct ttggcgctct
     3661 ttaatataat gccgataaag cagcctacgt cataaccaag ctgcttcggg ctgacatcaa
     3721 tacgcgcccc ggtaatgatc cccgcctgct tcattttctc tactcgaacg tgaatcgtcc
     3781 ccggactgac gccaaattgt ttcgccagtt cggcgtaagc ggtgcgcgca ttgcccatta
     3841 atgcttccag gatgcctcgg tccagattgt cgatcagata attttccata ggattttctt
     3901 atgcggattg atgattcatt ctattttagc cttctttttt aatgaatcaa aagtacgtga
     3961 ggctttttat tgaatgatta ttgcatgtgt gtcggttttt gttgcttaat cataagcaac
     4021 aggacgcagg agtataaaaa atgaaaaccg cttacattgc caaacaacgt caaattagct
     4081 tcgtgaaatc tcacttttct cgtcaactgg aagaacgtct ggggctgatc gaagtccagg
     4141 cgccgattct tagccgtgtg ggggatggca cgcaggataa cttgtcgggc tgtgaaaaag
     4201 cggtgcaggt aaaagtgaaa gctctgcctg atgcccagtt cgaagtggtt cattcactgg
     4261 cgaagtggaa acgtcagacc ttagggcaac acgacttcag cgcgggcgaa gggctgtaca
     4321 cgcacatgaa agcccttcgc cccgatgaag accgtctttc cccgttgcac tcggtctatg
     4381 tagaccagtg ggactgggaa cgcgtaatgg gcgacggcga gcgtcaattc tcgactctga
     4441 aaagcacggt agaggcgatc tgggcgggaa ttaaagcaac cgaagctgcg gttagcgaag
     4501 agtttggcct ggcaccgttc ctgccggatc aaatccactt cgtacacagc caggagttac
     4561 tgtctcgtta tccggatctc gatgccaaag ggcgtgagcg ggcgattgcg aaagatcttg
     4621 gcgcggtatt cctcgtcggg attggcggca agttgagtga tggtcatcgt cacgacgtgc
     4681 gcgcaccgga ttatgatgac tggagcaccc cgtcagagct gggccatgcg ggtctgaacg
     4741 gcgatattct ggtatggaac ccggtactgg aagatgcgtt tgagctttct tccatgggga
     4801 tccgcgtgga tgccgacacg ctgaagcatc agctggcgct gaccggtgac gaagatcgcc
     4861 tgcagctgga gtggcatcag gcgctgctgc gcggtgaaat gccgcagacc atcggcggcg
     4921 gtatcgggca gtctcgtttg accatgctgc tgctgcaact gccacatatc ggccaggttc
     4981 agtgtggtgt atggccggca gctgttcgcg agagcgtccc ttctctgctg taataattta
     5041 tcgccgccag cgtctgagca ggcggcttcg catcccggta tcaaagcgcc agatatgatc
     5101 gaaaatgcgc atgatgccgg gtttgccatg tgccgacatc gccacggcat gaaagcgatg
     5161 ctgatgtacc cgctgcaact ctttcacttt gctcgtcacg tcgtcaggca atcgctgggc
     5221 gataaaatca gaaatcacca ccgcatcggc atcaaaccat tcccggcttt gcaagcgttc
     5281 cataatggcg cgaaaacaac tggcaagatc ggtgccgcca cgaaactgct ggcttaaaaa
     5341 acggattgct tgttcgatgc cttgtgggcc tgaaagctca taacggacga tctcggtgga
     5401 aaatagcata atatagcagc gccggttttc agcgagggct atgcgcatca aggccaggca
     5461 gaacgctttc gcacactgtt cattaaagcc gcccattgag ccggaagtat ccacgcagac
     5521 aataaacggt ccgcgcggct gttcgtcgta atctttatgc accaccgggc gttcgatcac
     5581 tttttcacgc cacgactcac catgcaggcg ataggtgagc aactgttttt ccaccagccg
     5641 acggtaaaac tcatactcca gttccgttat ccctagtgtc gccagttctg gcggcaagag
     5701 acgtaaaata tcatcgcttt gctgcagacc atcaacctgc tcaggaactg tcgccggttc
     5761 gcgcaccatg gtgcggaagg tttccatctg cgcatcgttg cgcggtattg atttggcttc
     5821 ccgggaacgc cccagctgtt ctgccagacg tttcagttcc ggctgttcgt taagaaattc
     5881 accgtatttc acaatcaact gatagtcgcc acgtttaagc tgaccggcgc tcatatccca
     5941 cagacgacca gctgcggtat tgttatctgc gagaatcggt tcaagttgcc cgctcagcgt
     6001 catgcgttcc tgaacttcac tcaacagttg ttcgcgttct tcttctaata gctgctgatt
     6061 taacgtcgtt gcttgcacga tcaggcttaa acgccagcgc tggagaaaaa gcgtgtgtaa
     6121 cgctgaagtg atcgtactgt tagcatcaac caactgtcgg gcttgttctg cccatgggga
     6181 attcagacga tgcagtaagt ccaggatttg tggtagctgc acaataaact gcggcgtgga
     6241 gaggagctgg ctttgctgat agcacatcac ctcttcggtg agttccggcg ggactcgggc
     6301 atctttcatc cgactgcgca gcgcctcacg ccagcgggga acatcatcag tgattgccgc
     6361 cttcagtcgt gggaattttt caaagaagac agccagctgc ggtgaggcca gcagcgcgat
     6421 gatcatctct tcgatcaatc cctcttcgct gacggccagc atcacattaa gcgtatccag
     6481 cgttagcatt gttgtgcctg gcgaatctgt gcgccgacat cctgtaggct ggcttcaatg
     6541 cgacctaacc agtcgccagg gataaacaga catttttgct gttcgctgaa taaagcgtgt
     6601 tgcttgcgcc agtcgctttc aagctcttcc agttgttgtt ttatttcgct gggcagacct
     6661 tcagccgatg aaccgggaag tgctagcgta ctgccttgta aactcacatc gcgtacaacc
     6721 agatgttggg cgctatcgac ttccagattc agtttctggg caaagccgat accgttcagt
     6781 ttgccgcgaa tctcaccacc tttactcagc cactgttcca gcgcgctacg ctcaaaggag
     6841 atatgaacca cttccatatc atgcagtttt aacggttttt gcagcagcag agtcagagtg
     6901 gaagcagtaa cgttaacagg gagttgatac tgctgacgac ggctaaaaat gccgcccaga
     6961 cgaattaccg ttaaggctgt tttatcgctt tgttgctgct gaagttgcag gtgacgttgc
     7021 acaatcgcgc ccaggcgggt caacatccct tgctgttgcc aggcgtgacc ggtcatcaat
     7081 acatcaattt gttgttgtat caaattcagg ctttgcgcgt cataccacag gcaatctttc
     7141 agcaaaatga gatcaaccgg ggcgacagca ctgcgaccgc taaaaaaggc gctggcctgc
     7201 aataagcgga tcgctttttt ccagcgacga tccgagacat aaggcgcatc cggtaattta
     7261 tccagttgct ggcgcagcat aaaaatcagc tcaaatacat gatcgggcag cgtaatttca
     7321 ccaatctctt tctgccagcg ttcatattct tcatctgtga tctgcaagga ggcaggaacc
     7381 ggattgtcat tttcatcctg ttgactggtc agcatggagc ggaaattcgc tttatcctgc
     7441 actttatcta accacagacg aatcagcatg cggtcatata acgcttccag actgctgtct
     7501 gcttccggca gctcgttgga ggccgccacc agcagacgca tcgggatttt ttctacgagt
     7561 gcgccgttgc ggaactggcg ctcgttaatg gcggtgagca gggtattaag aattgccgga
     7621 cccgctttcc agatctcatc cagaaagacg atttcagctt ccggcaggta accgctggtt
     7681 aaacgttcat agcgcccttc atcttttagc gcctgaatag aaaggggacc aaaaacttct
     7741 tccggcgtgg agaagcgggt catcagatat tcaaacgcgc gggcattctg aaaggcgaat
     7801 tttaagcgcc gggcgatcaa acttttggca atacctggtg ggccaaggag gaacacactt
     7861 tcaccactta atgccgctaa taaacacaag cggatggcgt ggctacgttc ataaagcccc
     7921 ttttccaacg aactgctcag gcgggaaatt ctttccgcta ataaatgagg gtgagccata
     7981 atgaagtggc gtcctttcgt caaaagttct gcgtaaattg cgagtataga cgtttcttgc
     8041 tggtggctaa aatagtctca aaggaggggt atttttcttt gagccaggtt aatgtggccg
     8101 catttaggag tacgattttg ccgttaatcg tgcatactgt gcgctttttt gtgggccaag
     8161 ggactaagca cacatttcat atttcaacga aagactagtc tatgagcact gataataagc
     8221 aatcattgcc cgcgattacc ctcgcggcga ttggagttgt ctacggcgat attggtacca
     8281 gcccgttata tacacttcgt gaatgtttgt ccggccagtt tggttttggc gttgaacgcg
     8341 atgccgtgtt tggcttttta tcgctgatct tctggctgct aatctttgtg gtttccatta
     8401 aatatctcac cttcgtgatg cgggcagata acgccggtga aggggggatc ctgacgttga
     8461 tgtcgcttgc cgggcgtaat acgtcggcgc gaaccacatc aatgctggtg attatggggc
     8521 taatcggcgg cagctttttc tatggtgaag tcgtcattac tcccgctatt tcggtgatgt
     8581 ccgccattga aggtctggaa atcgtcgccc cgcagctgga tacctggata gttcccctct
     8641 caattatcgt tctcacatta ttattcatga ttcaaaaaca tggcacggct atggtcggta
     8701 agctgtttgc gccgatcatg ctgacctggt ttttgattct ggcagggctg gggttacgta
     8761 gcattattgc taacccggaa gtgctgcatg cactgaatcc gatgtgggcg gtgcatttct
     8821 tccttgaata caaaacggtt tcttttattg cattaggggc agtggtgctg tcgattacgg
     8881 gggtcgaggc gctgtatgct gatatggggc actttggtaa gttccctatt cgtctggcgt
     8941 ggttcaccgt cgtattgcct tccttaaccc ttaattactt cggccaggga gcgctgttgt
     9001 taaagaatcc ggaagcgatt aagaacccgt tcttcctgtt ggcaccggac tgggcgctga
     9061 tcccgctgct gatcatcgcc gcactggcga cggtaattgc ctcgcaggcg gttatctctg
     9121 gcgtcttctc attgacgcgt caggcggtac gtctgggata tttgtcgccg atgcgcatta
     9181 ttcacacttc cgaaatggag tcagggcaaa tctatattcc ctttgtgaac tggatgctct
     9241 atgtcgcggt cgtgattgtg attgtcagct ttgagcactc cagcaacctg gcggcggcgt
     9301 acgggattgc ggtgaccgga accatggtgc tgacgtctat tctctcgact accgtggcac
     9361 gtcagaactg gcactggaat aagtattttg ttgcgctgat cctgattgct ttcctttgtg
     9421 tcgatattcc cttgttcacc gctaacctcg ataaactgct ctccggcggc tggttgccat
     9481 tgagcctcgg tacggtgatg tttatcgtga tgaccacctg gaagagcgag cgtttccgct
     9541 tgctgcggcg gatgcatgaa catggtaact ctctggaagc gatgattgct tcgctggaga
     9601 aatcaccgcc cgttcgcgtg cctgggaccg cggtgtatat gtcgcgtgca atcaacgtca
     9661 ttccctttgc gctgatgcat aacctgaaac ataacaaggt attgcatgag cgggtgattc
     9721 tgttaactct gcgcaccgaa gacgcgccat atgtccataa cgtccgtcgg gtacagattg
     9781 aacaactgtc gcccactttc tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa
     9841 atgtggaaga agttttccac cgctgcggtc tggaaggatt aagttgccgg atgatggaaa
     9901 cctccttctt tatgtcgcat gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc
     9961 tgcgcggcaa gctgtacttg ctgctgcaac gtaatgcgct gcgggcaccg gatcaatttg
    10021 aaatcccgcc aaacagagtt atcgaactgg gtactcaggt cgaaatctaa cgccagacgc
    10081 ctcctttctc cataaggggg cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc
    10141 gaggttgatc acatttccgt aacgtcacga tggttttccc aactcagtca ggattaaact
    10201 gcgggtcagc gaaacgtttc gctgatggag aaaaaaatga aaaaaggcac cgttcttaat
    10261 tctgatattt catcggtgat ctccctactg ggacataccg atacgctggt ggtgtgtgat
    10321 gctggtttac ccatccccaa aagtacaacg cgtatcgata tggcattaac ccagggtgta
    10381 ccttctttta tgcaggtgct gggtgtagtc acaaatgaaa tgcaggtcga ggctgtcatt
    10441 atcgcggaag agatcaaaca acataatccg caactccacg aaacgttgct cactcacctt
    10501 gagcagctgc aaaaacacca gggaaacacc attgaaattc gttacaccac gcatgaacaa
    10561 ttcaaacaac aaaccgcaga aagtcaggcg gtaattcgca gcggagaatg ttctccgtat
    10621 gcgaatatca ttctctgtgc tggcgtgacg ttctgaggcc gtcatggaag cattacttca
    10681 gcttaaaggc atcgataaag ccttcccggg cgtaaaagcc ctctcgggcg cagcgttaaa
    10741 tgtctatccg ggccgcgtga tggcgctggt gggcgaaaac ggcgcgggta aatccaccat
    10801 gatgaaagtg cttactggta tctatactcg cgatgccggt acgcttttat ggttggggaa
    10861 agaaacgaca tttaccgggc cgaaatcttc ccaggaagcc gggattggga ttatccacca
    10921 ggaactgaac ctgattccgc agttaaccat tgccgaaaac attttcctcg gtcgtgagtt
    10981 tgttaatcgc tttggcaaaa ttgactggaa aaccatgtat gccgaagcgg ataaattgct
    11041 ggctaaactt aacctgcgct ttaaaagcga caagctggtg ggcgatcttt ccatcggtga
    11101 ccagcaaatg gttgaaatcg ccaaagtgct gagctttgag tcgaaagtca tcattatgga
    11161 tgaaccgacc gatgcgctga ccgataccga aaccgaatcc ttgttccgcg tcatccgcga
    11221 gctgaaattg caaggccgcg gtattgtcta tatctcccac cgcatgaaag aaatcttcga
    11281 gatttgcgat gacgttaccg tttttcgtga cggacaattt attgctgagc gcgaagtggc
    11341 atcactgacc gaagattctc tgattgagat gatggtgggc cgcaagctgg aagatcaata
    11401 tccgcacctg gacaaagcgc cgggagatat ccgcctgaaa gtcgataatc tctgcggacc
    11461 tggcgttaac gatgtctctt ttactttacg caaaggcgaa attcttggcg tctctggttt
    11521 gatgggcgct gggcgtaccg aactgatgaa agtgctctac ggtgcactgc cgcgcacaag
    11581 cggttacgtc accctggatg ggcatgaagt cgttacccgt tcgccgcagg atggcctggc
    11641 aaacggcatt gtttatatct ccgaagaccg taaacgtgac ggtttagtgc tgggcatgtc
    11701 agtaaaagag aacatgtcgc tgaccgcgct gcgctacttc agccgcgctg gcggcagttt
    11761 gaagcatgcc gatgaacagc aggctgtgag tgatttcatt cgtctgttta atgtgaaaac
    11821 gccatcgatg gaacaggcaa ttggtctgct ttccggtggc aatcagcaaa aagtagcgat
    11881 tgcccgtggt ctgatgacac gccctaaagt gttgatcctc gatgagccta cccgtggcgt
    11941 agatgtcggc gcgaaaaaag agatctatca actgattaac cagttcaaag ccgatggctt
    12001 gagcatcatt ctggtgtcat cggagatgcc agaagtatta ggcatgagcg atcgcatcat
    12061 cgtcatgcat gaagggcatc tcagcgggga atttactcgt gagcaggcca cccaggaagt
    12121 gttaatggct gccgctgtgg gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa
    12181 cccagactgt ctctggtcgc cgttatttca cgaaagcgtg gctgatggag cagaaatcgc
    12241 ttatcgctct gctggtgctg atcgcgattg tctcgacgtt aagcccgaac tttttcacca
    12301 tcaataactt attcaatatt cttcagcaaa cctcggtgaa cgccattatg gcggtcggga
    12361 tgacgctggt gatcctgacg tcgggtatcg acttgtcggt aggttctctg ttggcgctga
    12421 ccggcgcagt tgctgcatct atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg
    12481 ccgctctcgc gttaggtgcc gcaattggtg cggtaactgg ggtgattgta gcgaaaggtc
    12541 gcgtccaggc gtttatcgct acgctggtta tgatgctttt actgcgcggc gtgaccatgg
    12601 tttataccaa tggtagccca gtgaataccg gctttactga gaacgccgat ctgtttggct
    12661 ggtttggtat tggtcgtccg ctgggcgtac cgacgccagt ctggatcatg gggattgtct
    12721 tcctcgcggc ctggtacatg ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg
    12781 gcggcaatga agcggcaacg cgtctttctg gtatcaacgt caataaaatc aaaatcatcg
    12841 tctattctct ttgtggtctg ctggcatcgc tggccgggat cattgaagtg gcgcgtctct
    12901 cctccgcaca acccacggcg gggactggct atgagctgga tgctattgct gcggtggttc
    12961 tgggcggtac gagtctggcg ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat
    13021 taattcttgg cttccttaat aatggattga atttgttagg tgtttcctcc tattaccaga
    13081 tgatcgtcaa agcggtggtg attttgctgg cggtgctggt agacaacaaa aagcagtaat
    13141 aacgactaca ggacatcttg aatatgaaca tgaaaaaact ggctaccctg gtttccgctg
    13201 ttgcgctaag cgccaccgtc agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg
    13261 tctccacgct taacaacccg ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg
    13321 ataaacttgg ctataacctg gtggtgctgg actcccagaa caacccggcg aaagagctgg
    13381 cgaacgtgca ggacttaacc gtccgcggca ccaaaattct gctgattaac ccgaccgact
    13441 ccgacgcagt aggtaatgct gtgaagatgg ctaaccaggc gaacatcccg gttatcactc
    13501 ttgaccgcca ggcaacgaaa ggtgaagtgg tgagccacat tgcatctgat aacgtactgg
    13561 gcggcaaaat cgccggtgat tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg
    13621 agctgcaagg cattgccggt acatccgcag cccgtgaacg tggcgaaggt ttccagcagg
    13681 ccgttgctgc tcacaagttt aatgttcttg ccagccagcc tgcagatttt gatcgcacca
    13741 aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc ggatgttcag gctgtattcg
    13801 cgcagaatga tgaaatggcg cttggcgcgc tgcgcgcact gcaaactgcc ggtaaatcgg
    13861 atgtgatggt cgtcggattt gacggtacac cggatggcga aaaagcggtg aatgatggca
    13921 aactagcagc gactatcgct cagctacccg atcagattgg cgcgaaaggc gtcgaaaccg
    13981 cagataaagt gctgaaaggc gagaaagttc aggctaagta tccggttgat ctgaaactgg
    14041 ttgttaagca gtagttttaa tcaggttgta tgacctgatg gtgacataaa tacgtcatcg
    14101 acagatgaac gtgtaatata aagaaaagca gggcacgcgc caccctaaca cggtggcgca
    14161 ttttatggac atcccgaata tgcaaaacgc aggcagcctc gttgttcttg gcagcattaa
    14221 tgctgaccac attcttaatc ttcaatcttt tcctactcca ggcgaaaccg taaccggtaa
    14281 ccactatcag gttgcatttg gcggcaaagg cgcgaatcag gcggtggctg ctgggcgtag
    14341 cggtgcgaat atcgcgttta ttgcctgcac tggcgatgac agcattggtg agagcgttcg
    14401 ccagcagctc gccactgata acatcgatat ttctccggtc agcgtgatta aaggcgaatc
    14461 aacaggtgtg gcgctgattt ttgttaatgg cgaaggtgag aatgtcatcg gtattcatgc
    14521 cggcgctaat gctgcccttt ccccggcgct ggtggaagcg caacgtgagc gtattgccaa
    14581 cgcgtcagca ttattaatgc agctggaatc accactcgaa agtgtgatgg cagcggcgaa
    14641 aatcgcccat caaaataaga ctatcgttgc gcttaacccg gctccggctc gcgaacttcc
    14701 tgacgaactg ctggcgctgg tggacattat tacgccaaac gaaacggaag cagaaaagct
    14761 caccggtatt cgtgttgaaa atgatgaaga tgcagcgaag gcggcgcagg tactgcatga
    14821 aaaaggtatc cgtactgtac tgattacttt aggaagtcgt ggtgtatggg ctagcgtgaa
    14881 tggtgaaggt cagcgcgttc ctggattccg ggtgcaggct gtcgatacta ttgctgccgg
    14941 agataccttt aacggcgcgt taatcacggc gttgctggaa gaaaaaccat tgccagaggc
    15001 gattcgtttt gcccatgcag ccgctgcgat tgccgtaaca cgtaaaggcg cacaaccttc
    15061 cgtaccgtgg cgtgaagaga tcgacgcatt tttagacagg cagaggtgac gcttggctac
    15121 aatgaaagat gttgcccgcc tggcgggcgt ttctacctca acagtttctc acgttatcaa
    15181 taaagatcgc ttcgtcagtg aagcgattac agccaaagtt gaagcggcga ttaaagaact
    15241 caattacgcg ccatcagctc tggcgcgtag cctcaaactc aatcaaacac ataccattgg
    15301 catgttgatc actgccagta ccaatccttt ctattcagaa ctggtgcgtg gcgttgaacg
    15361 cagctgcttc gaacgcggtt atagtcttgt cctttgcaat accgaaggcg atgaacagcg
    15421 gatgaatcgc aatctggaaa cgctgatgca aaaacgcgtt gatggcttgc tgttactgtg
    15481 caccgaaacg catcagcctt cgcgtgaaat catgcaacgt tatccgacag tgcctaccgt
    15541 gatgatggac tgggctccgt tcgatggcga cagcgatctt attcaggata actcgttgct
    15601 gggcggagac ttagcaacgc aatatctgat cgataaaggt catacccgta ttgcctgtat
    15661 taccggtccg ctggataaaa ctccggcgcg cctgcggttg gaaggttatc gggctgcgat
    15721 gaaacgtgcg ggtctcaaca ttcctgatgg ctatgaagtc actggtgatt ttgaatttaa
    15781 cggcgggttt gacgccatgc gccaactgtt atcacatccg ctgcgtcctc aggccgtctt
    15841 taccggaaat gacgctatgg ctgttggcgt ttaccaggcg ttatatcagg cagagttaca
    15901 ggttccgcag gatatcgcgg tgattggcta tgacgatatc gaactggcaa gctttatgac
    15961 gccaccatta acaactatac accaaccgaa agatgaactg ggggagctgg cgattgatgt
    16021 actcatccat cggataaccc agccgaccct acagcaacaa cgattacaac ttactccgat
    16081 tctgatggaa cgtggttcgg cttagattta cgctgtcttt tgatcaaatt attaccatcg
    16141 gttgttttca gtagcatgaa cattgctgct gaagcaacag taataatgcc catcgtgata
    16201 aacgtatagt ggaattgttc gactgtcgtt gtgccttcca tcccttcgta aacgcgaagg
    16261 actgccgcac ttacagcaac gcctaaactg atcgacagtt gctgcgtgac cgccagaaca
    16321 ctgttaccgc tgctggcgtt gtcatcggtc agatcggcaa gtgtgatggt attcatcgcg
    16381 gtaaattgtg tcgacatagc catccctaat ataaacaacg gcaagatcag catccatatc
    16441 gccattgctg gtgattgcaa agagaactga gcgatcatta gcccaataat caccgtgatc
    16501 cccactaacg tatggcgata gcccagacga cgtaagactt gggtaaccat cgattttgca
    16561 ataatggaac ctaacgctgt cggtgccatc atacagccag caataaacgc ctgataccca
    16621 aatcctacct gtaacatcaa tggcataagg aacggtacac agccggtccc cagacgggtt
    16681 gcaatattgc ctacgatacc gatcgagaaa gtgcgggttt taaataaatc taatgaaatt
    16741 aatgggtttg gcgtgcgtcg cgcatggaga atatagagaa gcagtaaccc gatgctggtg
    16801 acaattaccg tcaaggcaat ccagctggcg acaatctttt ccccgaatag ctctattccg
    16861 cttgagaaga gaacaaggct gaggccaaac agcaaaaagc cagtggtatc gaatctgcgt
    16921 cgtgcggtgg tgaaattggg catatgtttg tgcgcgtaaa gaaggcccgc aatacctatg
    16981 gggatattga ttaaaaatat ccagtgccag gttgcccagg tgaccagcac gccgccaaga
    17041 acggggccta aaattggccc caccagaccc ggcatggcga caaaattcaa tacaggaaga
    17101 agttcattac gaggataagc gcgcagtaag gccagccgag caacaggcat catcattgcg
    17161 ccgcctatcc cctgaataac ccggaagaca accagctgtg gtagcgagtt ggaaagtgcg
    17221 caggccagag aacccaacgt gaacagactc acggcaaggg taaaaatgcg acgtgtaccg
    17281 aagcgatcgg ctagccatcc gcttaccgga ataagcatcg ccaccgtcag cgtataactg
    17341 atgatggctg attgcatcgc gaggggagaa cgattaaggc tatgagcgat tgcgggtaag
    17401 gcggtattca gaatagtggc atcaagtgcc tgcatgaaga aggccatcgc cgcgatccac
    17461 ggcaaacccg ccatactgcg cttcttttta tcgctcattc aatgtcctgt tatcgggtta
    17521 tcacttatct ggtgagcgta gcagcgcctg acaagcttta aatgccgcgt cgccatcgct
    17581 ttggataatc gcatcgacaa tcgcctggtg cagatccagc tttatcactg tgtcgctggt
    17641 aattgacgtg aagtaagtgt gataaaccga atggaatagc gaggcgaatg aggtcaaaaa
    17701 cggattggcg ctcatttcat agatatgctc atgccaggcc atatcgactt cgatccagcg
    17761 ttcacggcga aagttctctt ttaatgccgc catttcggcc attaacgtat tgagatgcgc
    17821 cttctgttcc gcggtgccaa ccgttgctgc cagtaagcaa gcttgcggct ccagacagat
    17881 acgcataacc agaaagtgat cgatgacctg atgaaagttc tcttctgtca tccaccaggt
    17941 aagcaattcc tgatcaagaa aattccagtt cgattgtggc atgactcggg taccaattcg
    18001 cggtcgcggt aaaaccatcc cttttgccgt taacgttttg accgcttcgc gtaccgctgt
    18061 acgactcact ccaaattgct cgcccagctc aatctcacca ggcaaaatgg tgccgggttc
    18121 atattcacct tttaagatcc gctgcgccag cttctcagcc agaacatacg aaaggttttt
    18181 ctgtgcagct aactgttgtg cgcttaaagg cattactaat cttccttttt ctttttattc
    18241 cttcttagta tgccaccagg aagtgtgatt acggttgcaa aaacggcaaa ttgcttgttt
    18301 tatggcacat taacggggct tttgct
//
LOCUS       DADSZD010000089        18165 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.089, whole genome shotgun sequence.
ACCESSION   DADSZD010000089 DADSZD010000000
VERSION     DADSZD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.089"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            57..1277
                     /locus_tag="J1Z28_002746"
     CDS             57..1277
                     /locus_tag="J1Z28_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBA7717568.1"
                     /translation="MSIYKIPLPLNILEAAKERITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPEDAITDPAYFPFYQSGMTFEHFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASSNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1250..1879
                     /locus_tag="J1Z28_002747"
     CDS             1250..1879
                     /locus_tag="J1Z28_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBA7717569.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFSDRQFSRAWTVK"
     gene            complement(1880..3040)
                     /gene="ybdL"
                     /locus_tag="J1Z28_002748"
     CDS             complement(1880..3040)
                     /gene="ybdL"
                     /locus_tag="J1Z28_002748"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBA7717570.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAITQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTMDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3149..4237
                     /gene="hcxA"
                     /locus_tag="J1Z28_002749"
     CDS             3149..4237
                     /gene="hcxA"
                     /locus_tag="J1Z28_002749"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBA7717571.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLLEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGVYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4247..4444)
                     /locus_tag="J1Z28_002750"
     CDS             complement(4247..4444)
                     /locus_tag="J1Z28_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBA7717572.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4557..6662)
                     /gene="cstA"
                     /locus_tag="J1Z28_002751"
     CDS             complement(4557..6662)
                     /gene="cstA"
                     /locus_tag="J1Z28_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBA7717573.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6843..7256)
                     /gene="entH"
                     /locus_tag="J1Z28_002752"
     CDS             complement(6843..7256)
                     /gene="entH"
                     /locus_tag="J1Z28_002752"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBA7717574.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGGDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7259..8005)
                     /gene="dhbA"
                     /locus_tag="J1Z28_002753"
     CDS             complement(7259..8005)
                     /gene="dhbA"
                     /locus_tag="J1Z28_002753"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase"
                     /protein_id="HBA7717575.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8005..8862)
                     /gene="entB"
                     /locus_tag="J1Z28_002754"
     CDS             complement(8005..8862)
                     /gene="entB"
                     /locus_tag="J1Z28_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBA7717576.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAEL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8876..10486)
                     /gene="entE"
                     /locus_tag="J1Z28_002755"
     CDS             complement(8876..10486)
                     /gene="entE"
                     /locus_tag="J1Z28_002755"
                     /EC_number="2.7.7.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="HBA7717577.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10496..11671)
                     /gene="entC"
                     /locus_tag="J1Z28_002756"
     CDS             complement(10496..11671)
                     /gene="entC"
                     /locus_tag="J1Z28_002756"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBA7717578.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRKRSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11860..12816
                     /gene="fepB"
                     /locus_tag="J1Z28_002757"
     CDS             11860..12816
                     /gene="fepB"
                     /locus_tag="J1Z28_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBA7717579.1"
                     /translation="MRLAPLYRNALLLTGLLLSGISAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSNVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLDRLKALF"
     gene            complement(12820..14070)
                     /gene="entS"
                     /locus_tag="J1Z28_002758"
     CDS             complement(12820..14070)
                     /gene="entS"
                     /locus_tag="J1Z28_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBA7717580.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14181..15185
                     /gene="fepD"
                     /locus_tag="J1Z28_002759"
     CDS             14181..15185
                     /gene="fepD"
                     /locus_tag="J1Z28_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBA7717581.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15182..16174
                     /gene="fepG"
                     /locus_tag="J1Z28_002760"
     CDS             15182..16174
                     /gene="fepG"
                     /locus_tag="J1Z28_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBA7717582.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDTPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLVADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16171..16986
                     /gene="fepC"
                     /locus_tag="J1Z28_002761"
     CDS             16171..16986
                     /gene="fepC"
                     /locus_tag="J1Z28_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBA7717583.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(16983..18116)
                     /gene="fepE"
                     /locus_tag="J1Z28_002762"
     CDS             complement(16983..18116)
                     /gene="fepE"
                     /locus_tag="J1Z28_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBA7717584.1"
                     /translation="MSSLNIKQGSEAHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDPLDLHRAIVALSEKMKAVDDNAS
                     KKKDESALYTSWTLSFTAPTSEEAQKVLAGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRHAMASRKQDAIMADHLV"
BASE COUNT         4312 a         5148 c         4653 g         4052 t
ORIGIN      
        1 ataattagcg gattcatctc acgaatcacc ccgtaacgtt cagagagcgt ttttagatgt
       61 ctatttataa aatcccgctt ccactgaata ttctggaagc tgcaaaagaa cgtattacct
      121 ggacacttaa taccctaccc cgtatctgtg tttctttttc gggtggaaaa gattccggat
      181 taatgctgca tctgacagcc gaaattgccc gacaaatggg caaaaaaatc tgcgttttgt
      241 ttatcgactg ggaagcgcag ttctcatgca ctattaacta tgttcagtca ttacgtgagt
      301 tttacgccga tgtcatcgaa gagttttact gggttgcgct cccgcttacg acgcaaaatt
      361 ccctttctca ataccaaccc gaatggcagt gctgggaacc tgatgtcgaa tgggtacgcc
      421 agcccccgga agatgcaata accgatcccg cttatttccc gttttaccag tcaggcatga
      481 cttttgaaca tttcgtgcgt gaatttgccg aatggttttc gcaaaaacgt cctgcggcga
      541 tgatgatcgg catccgggct gacgagtcct ataaccgttt cgttgctatc gccagttcga
      601 ataaacagcg ttttgccgac gataaaccct ggactacggc cgcaccaggc ggtcatagct
      661 ggtacattta ccccatctac gactggaaag tggctgatat ctggacctgg tatgctaacc
      721 atcaacagct ctgtaaccca ctgtataata taatgtatca ggcaggcgtc cctctgcgtc
      781 atatgcgaat ttgcgaacct tttggcccgg agcaacggca aggattatgg ctctatcacg
      841 ttatcgaacc ggatcgctgg gctgctatgt gcgcacgagt cagcggggta aaaagtggcg
      901 gcatttacgc cggacatgat aatcattttt atggtcatcg gaaaatcctc aagccagaac
      961 atttagactg gcaagaatat gcattattgc tgctcaatag catgccggaa aaaacggctg
     1021 agcattaccg caataaaatc gctatttatt tgcactggta tcagaaaaaa ggcatcgaag
     1081 ttccacaaac ccagcaaggg gacattggcg cgaaagatat cccctcctgg cggcgaatat
     1141 gcaaagtttt actcaataac gattactggt gccgggcatt atcatttagt cctacgaaag
     1201 cgaagaacta tcagcgttat aacgaacgga taaaaggaaa acgtcaggaa tgggggatac
     1261 tatgcaacaa cgattaacgc aggatttaac ccaatttctc gccagcttgc cggaagacga
     1321 tcgcatcaaa gcgattaatg aaatccgcat ggcgatccat caggttagcc cctttcgtga
     1381 agagccagtg gattgcgttc tgtgggttaa aaacagccaa cttatgccta atgattacaa
     1441 cccaaacaac gtggcaccac ccgaaaaaaa actgctgaaa aaatccattg aaatagatgg
     1501 ttttacccaa cccattgtgg tcacgcatac ggataaaaat gctctggaaa tcgtggatgg
     1561 ttttcaccgc catgaaattg gtaaaggctc atcatcgtta aaattacgct taaaaggtta
     1621 tttgccggtg acctgtctgg aaggtacgcg taatcaacgc atcgccgcga ctattcgtca
     1681 taatcgcgct cgtggacgtc accaaatcac tgccatgtca gaaatcgtcc gagaactcag
     1741 ccagttggga tgggacgata ataaaattgg caaagagctg ggaatggaca gcgacgaagt
     1801 gttgcgcctg aagcaaatta acggcctgca agagttgttt tccgaccgtc aattttcccg
     1861 cgcctggacg gttaaataac tacagctggc gcaggcgttc tgctgctgcc agcaacgtcg
     1921 attccttctt ggcaaaacag agacgaatca gtttatgtgg gaagggatcg gcgcaaaaca
     1981 ccgacagcgg aatcgccgct acaccgtgct cccgcgtcag ccactggcaa aactcaacat
     2041 catccatcgt agatacggcg ctgtaatcca ccagcaaaaa gtatgtacct tcgcacggta
     2101 aaatctccag ccgactttca tttaaggcat tcaccagaat atcgcgcttc tggcgataaa
     2161 agtccggtaa cgcaagataa tgctcaggtt ctgcacgtag catatcggca agcgccagtt
     2221 gtgccggggt attcaccgaa aaggtcagat actgatgcac tttgcggatc tcggcgctga
     2281 tgggcgcagg cgcaacgcaa taacccactt tccagccggt catatgatag gtcttgccaa
     2341 acgatgacac cgccaccgct cgctcacgca attgcggatg cgccagcacg ctggcatggc
     2401 cctgttgtga aaaattgatg tgctcgtaga cttcatcgct aatgacaaaa atctcgtgcc
     2461 cggcgatcgc ctgccacaga gcggcgaaat cagtctgctg ccagacagtt gcactggggt
     2521 tatgcggggt gttgaggatc accagtcggg tgcgctcgct taacaatgcg gcaaattcct
     2581 gccagtcaac gcgaaaatgc ggcggttgca gtgcaatacg tttcactatt ccccccgaaa
     2641 gtgcgatggc gggggcgtaa ctgtcatagc tgggatcaaa acaaatcact tcgtcgccat
     2701 tgcgcaccaa tgcggttatc gccgcgtata acgcctccgt cgcccctgcc gtcacggtga
     2761 tattgctgtc gacatccggt tgatagccat acagacgttc cgttttctga gtaatcgcct
     2821 cgcgcaaggc ctgcacgccg gtcatgggtg cgtattggtt tgccccttgg gcaacatggt
     2881 acgccagccg ctcctgtaaa tagcgcggac catcaaaatc aggaaagcct tgcgaaaggt
     2941 taatggcctg gtgttgctgc gccagcgcgc tcatccgggt gaaaatagtg gtgccaagtt
     3001 gtggaagttt gctttgtgga atcagagggt tatttgtcat ttttattgta cctgacgaag
     3061 ttgcgtgttg aaggcactat aacacgatgc tagtatttgg caatcaagac gtttagatgt
     3121 ctaaataaaa caataaggac aacacaacat gcctcacaat cctatccgcg tggtcgtcgg
     3181 cccggctaac tacttttcac atcccggaag tttcaatcac ctgcacgatt ttttcactga
     3241 tgaacaactt tctcgcgcgg tgtggatcta cggcgaacgc gccattgctg cggcacaaac
     3301 caaactgccg ccagcgtttg aactgccagg ggtaaagcat attttgtttc gcggccattg
     3361 cagcgaaagc gatgtacaac aactggcggc tgagtccggt gacgatcgca gcgtggtgat
     3421 tggcgtcggc ggcggcgcac tgctcgacac cgcaaaagcc ctcgcccgcc gtctcggtct
     3481 gccgtttgtt gccgttccga cgatcgccgc cacctgcgcc gcctggacac cgctctccgt
     3541 ctggtataac gatgccggac aggcgctgca ttatgagatt ttcgacgacg ccaattttat
     3601 ggtgctgctg gagccagaga ttatcctcaa tgcgccgcaa gaatatctgc tggcggggat
     3661 cggtgacacg ctggcgaaat ggtatgaagc ggtggtgctg gctccgcaac cagaaacgtt
     3721 gccactaacc gtgcggctgg gaatcaataa tgcgcaggcc attcgcgacg tcttgttaaa
     3781 cagtagcgaa caggcgcttg ccgatcagca aaatcagcaa ttaacgcaat cattttgcga
     3841 tgtggtggat gccattattg ctggtggtgg aatggtcggt ggtctgggcg atcgttttac
     3901 gcgtgtggcg gcggcccatg ctgtgcataa cggtctgacc gttttgccac aaaccgagaa
     3961 gtttctccac ggcaccaaag tcgcctacgg aattctggtg caaagcgcct tgctgggtca
     4021 ggatgatgtg ctggcgcaat taaccggggt gtatcagcgt tttcatctgc cgactacgct
     4081 ggcggagctg gaagtggata tcaataatca ggtggagatc gacaaaatga ttgcccacac
     4141 cctacgtccg gtggaatcca ttcattacct gccagtcacg ctgacaccag ataccttgcg
     4201 tgcagcgttc gaaaaagtgg aatcgtttaa agcctgacgt acaaatttag cagcagcgcg
     4261 cgccgccttt tccaccgtag cgcgcgtcct gccgctcacg gaaaaactcc tcgtaggtca
     4321 tcggcgtttg atcgggatgg ttaacccgca tatgttcgac atagttgtcg taatcaggca
     4381 taccaatcat cagtttcgcc gcctgaccta aatattttcc ggcttttgcc agtgaatcaa
     4441 acatctaaac ctccggtaat acaaatgccc tcctgaatta agagggctta attgctatca
     4501 acacgctcgg gatgagagta aggggagcgc tcagcacatt ccccctcact ctgactttag
     4561 tgcgcgcctt tcgcctgcgc cacgatctcc tcgacatttt ccggcatcgg ctcatatggc
     4621 gtttctttcg ccgttggctt cggctctttc aatgccgcca gtgccgtctt aatcgagaac
     4681 agcgccagaa ccaccacaac caccataaag aagatggtta atccggcatc cagacggttg
     4741 ttgaacacca gttgcgccag ttgtgactca gtatactgcg acggaatatt gccgctgtcg
     4801 atcattgcct ggaacttgtt agcaatcgcc aggaagccga ctttcgcatc cgggctaaac
     4861 gctttctgcc agcctgcggt caaggtacaa atcagcagcc aggccgttgg taccagcgcc
     4921 acccaggcgt aacgttgacg tttcattttg aacaacacca cagcacagag catcagcgcc
     4981 atccctgcca gcatctggtt ggcaatacca aacagcggcc acaaggtgtt aataccgccc
     5041 agaggatcga ccacgccctg atgcaggaag tagccccacg ccagcacgca cagcgccgtt
     5101 gccagcaggt tagcaggcag tgaatcggtc cgtttcaggc ccggagacac cacgcccagc
     5161 agatcctgca acataaagcg cgcagcacgc gtacccgcat ccaccgccgt cagaataaac
     5221 agtgcttcaa acagaatggc gaagtgatac cagaacgcca catccatcat gccgcccagc
     5281 gcaccgtgca gaatgtaggc catccccacc gccagggtcg gcgcaccgcc cgcacgagaa
     5341 atgatcgact gttcacccac ttcgctggca atctggttta gcgtatccgg ggtaatcgca
     5401 aagccccagc tactcaccac ctgcgcggca gaagcaacca catccgccgt ccctgccgga
     5461 gccagcaccg ccatcgggct gttcatggcg aagtacacac ccggatcgat gatacaggcg
     5521 gaaaccagcg ccataatcgc cacgaaggat tccatcaaca tcccaccgta gccgataaag
     5581 cacgcctgcc cttcgttcgc cagcatcttc ggtgtagtgc cggaagagat cagtgcatgg
     5641 aagccagaca ccgcgccaca ggcaatggtg ataaacagga acgggaacag gttaccggtc
     5701 cataccgggc cagtgccatc gacaaattta gttagcgcag gcatggtcag cgtcgggcgc
     5761 ataatcaaaa tgcccaccgc cagaccgacg atagtcccga ttttcaggaa ggttgagagg
     5821 tagtcacgcg gagccagcag caaccacacc ggcagcaccg ccgccacaaa accgtaaccc
     5881 accagcatcc aggtcagctg cacgccggta aagtcaaagt acggtgccca ggtcggactt
     5941 tctgccaccc agccgccaga gataatggcg aaaatcagga acaccagacc aatcaccgac
     6001 acttcgccaa tacgccccgg acgcagataa cgcaagtaga tccccataaa cagcgccagc
     6061 ggaatggtga acgcaacggt atacgttccc cacgggctat gagtcagggc tttaaccacg
     6121 atcatcgcca gcacggcaag gataatgacc atgatcataa agcaggccac cagcgctatc
     6181 accccggcgg ttggccccat ctcttctttg accagctcac ccagcgagcg accgtcacgg
     6241 cgcgtggaaa caaacagcac catgaaatcc tgcaccgcac cggcgagaac cacccctgcg
     6301 agcagccaga tcatccctgg caggtaaccc atttgcgccg ccagtaccgg cccaaccaac
     6361 ggacctgctc cggcaatggc cgcaaaatgg tgaccgaaca gcactttttt gtccgtcggc
     6421 acatagtcca gcccgtcgtt atggcgcacc gctggcgtca tacgcgtcgg gtcaaccgcc
     6481 agcacatttt tggcgatata cagcccataa aaacggtaag cgatcagata gatacagacc
     6541 gacgccacca caatccacag cgcgttgatc tgttccccac gatttaaagc aatgtatccc
     6601 agagcaaatg ctcccattac agagagcact gtccagacga ggtatttccc tgatttgttc
     6661 atagttgtta tccgtgtacg tgtccataga gatgttacat tttgtttcta taacatcttt
     6721 gtacaattta acaacacagc aaccatgtaa atcagagtta aaaccaatat ttaacttaac
     6781 aacgttaact cgatcactcc gttaaccgaa ttttcctgca atctcatttt attctgtcgg
     6841 gttcatccca aaaccgctgt gcccagccga caggtgcagc aacgccgccc ctgttcatcg
     6901 aaaacgacga tttcccagct ctgattttgt cgtccaagat gcagcggctg gcagacaccg
     6961 cgcacctttc cctcagacac cgggcgatga tgtgtggcgt taagttccgt gcccaccaca
     7021 cactgtccgt cgcgggtcat cataaatccg gccatcgatc ccagcgtttc cgccagcgcc
     7081 gccgacgcgc cgccatgcag caggccaaac ggctgatgag tacgggtatc aaccggcatt
     7141 tcggcttcca gcacatcacc gcccagacgg gtatacacaa ttcccagatg cgccaccatt
     7201 gtgttatcgc tggtggcgtt cagctcgtcg agcgttaaat ggcgtttcca gatcatgctt
     7261 atgcccccag cgttgagccg ccatcgacca caatatcctg tagggtaata tggctggcga
     7321 ggtcagaggc gaggaacaaa atcgtgttgg cgatctcctg cggacgggcg attttcccta
     7381 gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat acgctgctct tcggcgtcat
     7441 cgctcaccca cagcgtgcgt tgcatatcag tgtcggtgga gcccggcgaa accacattac
     7501 agcgcacgcc gctacccgcc agttccagcc cgacgctcaa cgccaggctt ttcagcgccg
     7561 ctttcgacgc gccataagca ctcataccaa tgcgcggcgt gtgtgcggcg tcggacgcaa
     7621 cagtaacaat cgccccgccc cgctgacggc gaaactggtt catggtctgc tgaaacaggt
     7681 taaatgcacc gccgacgttg accgcaaaag tctgctgcca gtcctcttta ctgagctgat
     7741 cggtcgcgcc catgcgcaaa attcccgccg cattgaccag cacgtccagt cgctccgttt
     7801 cagctaacag tcgctgacat acctgcgcca cctgcgcagc atcggcaaca tccattactt
     7861 cggtcgcaaa aggatattgc tcctgagtaa acgcttgatc aaaacctgta actttcgccc
     7921 ccgcctcaac aaatgccagc gccgtggcat agccgatacc tttccccgcg ccagttaccc
     7981 agacattttt accgctgaaa tccattattt cacctcgcgg gagagtagct tccaccaggc
     8041 gtcgatggtc gggtttttcg ccagcatgac aaagtcgata tcgccatgca ctttgcgcca
     8101 gcgcgccgcc agcgccatca tgcgcaccga atccagaccg tagtcgatca ggttgtcgtc
     8161 atcgaagggt tcatcggact cgtccagcaa cggcaggatc acctcacgca gctccgcttt
     8221 gctggcgggg acaggtgctg gcagcaattc ttcggtcatc accacccgac cagaacgtcc
     8281 ggccacatat ttcagcgaca tcaaatgctc gtcacggctg aaatcggcca gcgcatccgc
     8341 caccataaac ggtttaatat cgcgcataaa tgcgtccgtt gcggtggtca tacagccaat
     8401 gtgggcatat accccggtaa taatcagctg gttacgtcca ctctctttga gcatttgctc
     8461 cagcggagaa cgatgaaatg cgctgtagcg ccatttcacc agcaccgtgt cgtcggcatc
     8521 tggcgtcagg cgatccacca ccttttgctg ttccggcgaa cgggtcaggc caggccccca
     8581 catatcattc aacagcgccc gatcttcatc gctctgctct ttcggctggg cggtgtaata
     8641 aaccgggata ttgtgctgtt tgcagtagtc gcgtagtgcc gcaatattcg cgatcacctg
     8701 ctccatcatc gggcagttct caccccagaa gctgacaaaa tagtcctgca tatcatggat
     8761 taacaacgcg gcacgttgcg gttcaaaggc ccagtcgact ttattctgcg gaatatcgtg
     8821 agactccggc agtgcgtaag cctgtaattt tggaatagcc atcgtgttct ctccttcagg
     8881 ctgatgtgcg tgacgccagc cactgacgta attgtttttt atcgactttc ccgaccgccg
     8941 tcagcggaag tgaatctaca cactccacgc gatccggtaa tttaaattcg gcaataccct
     9001 gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg ctcttttacc accagataag
     9061 cacagctttt ttcgcccatc agctcatctt ccatgctcac cagtgcggcg tagatcaccg
     9121 ccggatggcg cagcagcagg ttttcgatct cttcggcagc gatcttctcg ccgccacggt
     9181 tgatctgatc tttctcgcgc ccctgcacag tgatgtaacc ctctggatca atagagatca
     9241 gatcgccgga acagtaaaaa ccgttggcat caaaggcgct ggcattgtgc tgcgggcttt
     9301 tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag gcgtccaact tccccttgcg
     9361 gcagtggatt tccttcggca tcggcaaccc atacttcatc gtccgggcac attgggtaac
     9421 cctgggtatg gataattttc tccgcactat catcaagacg ggtgtagttc accagccctt
     9481 ccgccatgcc aaacacctgc tgcaactgac agccaatctc agcgggaata cgcgccgcaa
     9541 gggtggcaga aagacgggcg ccgccgacct gtaacagttt cagcgaggca agctgcgccc
     9601 ggctttcgcc ttcagccagt gcctgcaacc acaggctgac tgctggcggc accagcgcgg
     9661 tgacgttcac ctgatgtttt tcaatcaatg ggaagcaaag cgtggcgctg gggtcggcag
     9721 ccagaacgac agtgccgcca gcgagaaata cccccaacga tcccggtgaa ctcatggcgt
     9781 agttatgagc cgccgggatc gcgcacaggt agcgcgtctg ttgtgtgaac tgacaaatct
     9841 cgacgctacg acgcacgctg tagtagtagt cgttatgagt gcgcgggatc agtttcggtg
     9901 tgccggtggt gccgccggaa agctgaaaat aggccacttc atcggcaggt gacggcgtgg
     9961 cagtaaaatc ctcagccgga tggttaatcg catcctgcaa gttatgctcg ccgctgtcgt
    10021 tgagcagctg caccacgcga atggaagaat gctctgcaac aaatgtgttg aggaaatcat
    10081 ccccgctaaa tagcgcatgt tggcgatcgg caatcagtaa cgccggttca atctggctgg
    10141 cataggcgtt cagttcacta cgctgatggc tgaacagcgc cagcaccggc gcaacgccca
    10201 gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac gttacccagt tgtaccagcg
    10261 cggtttcacc aggtttaatg ccctgacggc gcaaactaca ggcgaggtta tccgccgcct
    10321 gattcagctc ccgataactc aactgccgct cgccgtcgat aaccgcgatg ctgtcactcg
    10381 cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg ccagtagcct ttttcccgat
    10441 agcgacgggc aaactcttcc ggccagcggg tgaatggaat gctcatcctc gctccttaat
    10501 gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc cgtttcgcgc cactcaccca
    10561 acggggagga cgcaggcaca atccccgctc cggcaaacag acgcacctga ttttcccgca
    10621 gcttcgcgca gcggatggtc accacccatt caccgttacc ttcgctgtca caccaaccca
    10681 caatgccgcc aaacagttcg cggtcgaacg gctccagttc agcaataacc tgagtcgcgg
    10741 cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag cagacaagcc aaagtcagtg
    10801 cgttttcttg cgaattcgct ttaccttcaa agggagttgc gagatgccac agcgtcggcg
    10861 tggtaatcaa ttgtggagag gagggaacgt gtaactcact actgcgtttg cgcagtacct
    10921 ctttcatcgc ctgagtcacc agttcatgtt catggcgatc tttttctgac gccagcagac
    10981 gattacccgc ttcgcgatcg agcacttcat ccggctgacg acgcgcggaa ccggctaacg
    11041 gaatggagct aaaacgctcg ccgtctttac gtagcagcag ttccgggctg gcccccagca
    11101 gaacgccacc atcagccaac ggaacatgga agttgtaact aaccgggttt tgcgcaatca
    11161 accgttccag caatacgcca ctatcaatgg cggcgtcagt agtgatatca atcaaccgtg
    11221 acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc ggcagcacgg gcaaccatct
    11281 gttcaaacgt ggtttgttcc ggaatggcct gacgttccac cacgttcagc gactggctgc
    11341 gggtgaaacg gcgtgcggaa gcttgttttt cctgacggga gaacgactgc caggattcag
    11401 gtatatacag cgacgaaggc tgacgtggat cgaagggaat cgccccgacc atcaccggat
    11461 ttttgatgcc ctgcgctttg gcatcggcaa acaacgcggc gagtttttgc tggaagggac
    11521 tgtcgggcga atccccgttc acagccggtt catcgaagcg ggcgaaacat cctgacgtcg
    11581 taaaactgcg gtacggcgac ataaagaaaa agcgattggg cgcaagtgtt gccatggtct
    11641 gctgtacttc ctcagccagt gacgtatcca tatcatcctc cacaaaatga taaaggcttt
    11701 aataatgatt atcatttata ttttcggtcg ctaacctaaa agcaaacgcg cactatgtca
    11761 actcttgagg taacgcaatc tgcaactcgg ggttgcatct ggttgtggtt acaatgaaaa
    11821 tgagaagcat taacttcatt aataacagga agctgacttg tgagactcgc cccgctctac
    11881 cgcaacgccc ttctattaac aggacttttg ctttcaggaa tatccgcagt tcaggccgcc
    11941 gactggccgc gtcagattac tgacagccgg ggcactcata ccctggaaag ccagccgcag
    12001 cgtattgttt ccaccagcgt caccctgacc ggctcactgc tggcgattga tgctccggtg
    12061 atcgccagcg gcgcgaccac gccgaataac cgcgtcgcgg atgaccaggg ctttttacgc
    12121 cagtggagca acgtggcgaa agaacgcaaa ctgcaacggc tctatatcgg cgaaccgagc
    12181 gccgaagccg ttgccgcgca aatgccggat ttgattttaa ttagcgcaac cggcggggat
    12241 tcggcgctgg cgctgtacga tcagctttct accatcgccc caacattaat catcaattac
    12301 gacgacaaaa gctggcagtc gctgttaacg cagcttggcg aaattaccgg gcatgagaaa
    12361 caagcggcag agcggattgc acagtttgat aagcaactgg cggcggcgaa agagcaaatc
    12421 aaattaccgc cgcagccggt cactgccatc gtctataccg ccgctgcaca cagtgccaat
    12481 ctctggacgc cggaatcagc acaagggcag atgctggaac aactcggctt tacgctggcg
    12541 aagttgcccg caggcttaaa cgccagccaa agccagggca aacgccatga catcattcag
    12601 cttggtggag aaaatctggc tgcagggtta aatggcgagt cactattcct gttcgccggt
    12661 gatcagaaag acgccgatgc tatttatgct aatccgctgc tcgcgcacct gcctgcagta
    12721 caaaacaagc aggtttatgc gctgggaacc gagacgttcc gcctggatta ctacagcgcc
    12781 atgcaagtgc tggatagact taaggcgctg ttttaagcat taactgtcgg acgctgtcac
    12841 ctgcggcggc gtctggcgaa aacgtcgcaa ctccaccagc accagcagca acaacacgcc
    12901 gataatcaac aaaccaaaac cgctcgcgct tgcggaagca accggtgtca tcatcgctcc
    12961 caaaccgccc agcagcgccg cgcctatggc atcgcccgtc acgttttgcg ccgtccacaa
    13021 accgttaatc cgccctaaca tcgcttccgg ggtttgcgtt tgcagcattg tatattgcag
    13081 caacgaacta accgcgctca gccagccgaa cagcgccaga caaaccacgc ctaaaatcca
    13141 catcggcatc aggccaaaca gaccaatggc gaggaacgcc cccaatgtgg agagcaacat
    13201 caacagacct ggccgtacgc tatgcgccag ttttccactg gttaacgcgc caatagccgc
    13261 accgagcggg attgccgcat agagaaagcc aatttgcgcc gccgacatct gccagttgtc
    13321 agccagcgcc ggatacagta cccgcaccgc gctcgccatc gtcaataaac cccccagcaa
    13381 cgcaatccct cccaccagcg ggctggcgag tagaaaacga aatcctgcca gtaatgattt
    13441 caacggatgc tcacgcggct gcggcggtgg tggcaacgcc ggaaggctta acaacggtag
    13501 caaggtaata aatgtgcccg ccgccgccag cccgtagttc caggctacgc ctccggtcgc
    13561 cagcagtaaa ccgccaatca tgggcgaaat caccgatccc agacgcacag tcaacatggt
    13621 gatcgccccg gcctgcatta ggttttcacg ccctaccagt gcaggtgtcg ccgccagtag
    13681 cgccgtaacg ccaagtgatg cgaaaaaacc atcccataaa ccaagtaaat agattgccag
    13741 caaagacggc tccggcagca gcgcattcag gcacagtcca atgaagccaa tgccacaggt
    13801 gccgcgcgcc agcaaaatca cttttttacg ctcatagcga tccgccagca caccgccgac
    13861 catcaggcca acaaacatcg cgccgccggt cagcgtcacc gaaagcccca cctgccaggt
    13921 ggaatgcgtc atcatctgga tctgcaccgg caccgcgacg ccgagcaaac ccagagaaac
    13981 aattgagata aaacgagcga ggaatactgc gcgaaacgcc gggtgcgttt tcaacaggct
    14041 gaggttaagc agccaggatt gtttattcat tacaatgcct tgccatcaaa tattttaaat
    14101 atctattctt aggctgcaca tgctaacata tccaaataag atcgataacg ataattaatt
    14161 tcattatcat ggaagttcgt atgtctggtt ctgttgccgt gacacgcgcc attgccgtgc
    14221 ccggattgct gttattactg attatcgcga cggcattaag cctactcatt ggggcaaaat
    14281 cactccccgc ttccgtagtg ctggaggcgc tctccggcac ctgccagagc gccgactgca
    14341 ccattgtgct cgacgcccgt ctgccgcgca cccttgccgg tttactggca ggcggtgcgc
    14401 ttggccttgc cggggcgtta atgcaaaccc tcacccgaaa cccgcttgcc gatcctggtt
    14461 tgcttggcgt gaacgccggt gccagttttg ccattgtgct gggcgcggcg ctgtttggtt
    14521 actcttccgc gcaggagcaa ctggcgatgg ccttcgccgg ggcgctggtg gcctcattga
    14581 ttgttgcctt taccggcagt cagggcggcg ggcagttaag tccggtgcgt ttaacccttg
    14641 caggcgtggc gctggcggcg gtgctggaag ggctgaccag cggcatcgcc ctgcttaatc
    14701 ccgacgtcta cgatcaactg cgtttctggc aagccggttc gctggatatt cgcaatctac
    14761 ataccttaaa agtggtgctg atcccggtgc ttaccgctgg agcaaccgcg ctattactga
    14821 gccgcgcgct gaacagttta agccttggca gtgacaccgc gacggcactg ggcagtcgcg
    14881 tagcgcgcac acagttgatt ggtctgctgg caattaccgt gctttgtggt agtgcgacgg
    14941 cggtagttgg cccgattgcc tttattggcc tgatgatgcc gcacatggcg cgctggctgg
    15001 taggtgccga tcatcgctgg tcgctgcccg tcacgctact cgctacccct gccctgctgc
    15061 tgtttgccga tatcattggg cgggtgattg ttcccggcga actgcgcgtt tctgtagtca
    15121 gtgcctttat tggcgcacca gtgctgatct tcctcgtacg acgtaaaacg cgaggtggcg
    15181 catgatttac gtctctcgcc gattaattat cacctgtctg ctgttgctaa tagcttgtgt
    15241 gatggcaggc gtgtggggat tacgtagcgg tgccgtcacg ctggaaactt cgcaggtatt
    15301 cgccgcgttg atgggcgaca cgccgcgcag tatgacgatg gtggtcaccg aatggcgttt
    15361 accacgcgtg ctgatggcgc tgttgattgg cgcggcgctg ggcgtcagcg gcgcgatttt
    15421 tcagtcgctg atgcgtaacc cgctcggcag ccctgacgta atgggcttta acaccggggc
    15481 gtggagcggc gtgctggtgg cgatggtgct gtttggtcag gacctgacgg ctatcgcgct
    15541 ggcagcaatg gtgggcggca ttgtcacttc gctgttggtc tggctgctcg cctggcgtaa
    15601 cggtatcgac acctttcggt tgattattat cggtatcggt gttcgcgcca tgctggtggc
    15661 ctttaatacc tggctgttgc tgaaagcgtc tttagaaacg gcgctaacag cgggtttgtg
    15721 gaatgccgga tcgctcaatg gcctgacgtg ggcaaaaacc tcgccttccg cacccatcat
    15781 tatattaatg ttaattgccg ccgccttgct ggtgcggcga atgcgcttgc tggaaatggg
    15841 cgatgatacc gcatgtgcgc tgggcgtcag cgtcgaacgt tcgcgactgt taatgatgct
    15901 ggtcgccgta gtgcttaccg cagctgccac tgccctggcg gggccaattt cctttattgc
    15961 tttagtcgcg ccacacattg cccgccgcat cagtggcacc gctcgctggg ggctgaccca
    16021 ggcggcgctg tgcggggcgt tgttactgct ggtggccgat ctctgcgccc aacaactatt
    16081 tatgccgtat caacttccgg ttggcgtcgt taccgtcagc ctcggcggta tttaccttat
    16141 cgtcttgtta attcaggagt ctcgcaaaaa atgaccgaat cagtagcccg tttgcgcggc
    16201 gaacagttaa ccctcggata tggcaaatat accgttgcgg aaaatctgac tgtagaaata
    16261 cctgatggtc acttcacggc aattatcggg ccaaatggct gcggtaaatc cacgttactg
    16321 cgtaccttaa gccgcctgat gacgcctgct cacgggcatg tctggctgga tggcgagcac
    16381 attcaacatt acgccagtaa agaggttgca cgccggattg gtctgttggc gcaaaatgct
    16441 accacaccgg gcgatatcac cgtgcaggag ctggtggcgc gtgggcgtta cccgcatcaa
    16501 ccgctgttta cccgctggcg caaagaagat gaagacgcgg taacaaaagc gatgcaggcc
    16561 acgggaataa ctcatctggc ggatcaaagc gtggacaccc tttctggcgg acaacgccag
    16621 cgagcgtgga tcgcgatggt gctggcccag gaaacggcaa ttatgctgct cgatgaaccc
    16681 acgacctggc tggatatcag tcatcagatt gatttgctgg aactgttaag cgaactgaac
    16741 cgcgagaaag gctataccct ggcggcggtg ctgcacgatc ttaatcaggc ctgtcgttac
    16801 gccagccatt tgattgcatt gcgggaagga aaaattgttg ctcagggagc gccgaaggag
    16861 attgtcactg ctgaactgat cgagcgcatt tatggtctgc gctgcatgat tattgacgat
    16921 ccggttgccg gaacgccgct cgtggtgccg ctcggacgaa cgacaccttc aaccgcgaag
    16981 atttaaacta agtggtctgc cattatggca tcctgttttc tggatgccat cgcatgacgc
    17041 aacagcacac caccacaagc caccatcccg ccgatcaacg cggaaaggat cacgataata
    17101 gccttacccg gaccgtcttt tttcactggc aatgacgggc ttaactgata tttaaacggc
    17161 gtaaaattca catcgttgat atttgctttt gttaactgct cgacaagata ctggcgatta
    17221 cgtaattcac cgttcaattc cgcaacgtca gtaaccgctt tttcaatttc cagtttgcgt
    17281 tcaataccgt ctgcaccgag agaaatagag aaatcagggt catctttaac ggcctgacca
    17341 ttactgtata cgggcttttt aattcctgcc gcgttggcaa tgtcaagtga ataattgagg
    17401 cgctgaatgt ttgcatcaag ttgatttttc gttttaatgc gatcctgagc cagtttttct
    17461 ttttcaaact gggttttgat ctccagttta ttacggacgt tttctatcga ctctttcacc
    17521 accaacgcag agatataatc gatatacccg gccaacactt tctgtgcctc ttcactggtg
    17581 ggtgcggtga aacttagcgt ccaggaagta tacaatgcag actcatcttt tttcttactg
    17641 gcattgtcat caaccgcttt cattttttcg ctcaatgcaa caatggcgcg atgcagatcc
    17701 agtgggtcga ttttcgcttc ttttaattga tccatcacat aaggtgatga acgcaggtac
    17761 tcctcaagca agctaacaga ctgaaatttc ttgataaaca ggttaaatgc ttctgtacga
    17821 tcaattttga tatccagatc cagcacacga agcttagtga atgttttctc cagctcttgc
    17881 cactgaacag gttctggagg cgtgacaacc gccgcgctag tccatttttg cggcaggatg
    17941 aaagagatca gcaagcctgc gcaggcaaac gcaaaaacga ccgccatgac cgtttttttt
    18001 gcccgccata aaacctcgat tagattaagt aaatcaattt cattattact gggcgacgcc
    18061 agaggatact cgggaaaatg agcttcactt ccctgtttaa tattcagtga tgacatgtag
    18121 tgaaacctga ggttttagtc cgtaaagcac aacgatgtac atagc
//
LOCUS       DADSZD010000090        17893 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.090, whole genome shotgun sequence.
ACCESSION   DADSZD010000090 DADSZD010000000
VERSION     DADSZD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.090"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..599)
                     /gene="purN"
                     /locus_tag="J1Z28_002763"
     CDS             complement(<1..599)
                     /gene="purN"
                     /locus_tag="J1Z28_002763"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBA7717585.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDG"
     gene            complement(599..1636)
                     /gene="purM"
                     /locus_tag="J1Z28_002764"
     CDS             complement(599..1636)
                     /gene="purM"
                     /locus_tag="J1Z28_002764"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBA7717586.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1960..2586
                     /gene="upp"
                     /locus_tag="J1Z28_002765"
     CDS             1960..2586
                     /gene="upp"
                     /locus_tag="J1Z28_002765"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBA7717587.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            2672..3961
                     /gene="uraA"
                     /locus_tag="J1Z28_002766"
     CDS             2672..3961
                     /gene="uraA"
                     /locus_tag="J1Z28_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBA7717588.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            4011..4757
                     /gene="hda"
                     /locus_tag="J1Z28_002767"
     CDS             4011..4757
                     /gene="hda"
                     /locus_tag="J1Z28_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBA7717589.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4895..5254)
                     /gene="arsC"
                     /locus_tag="J1Z28_002768"
     CDS             complement(4895..5254)
                     /gene="arsC"
                     /locus_tag="J1Z28_002768"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBA7717590.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEVLIQAMVENPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(5275..6738)
                     /gene="bepA"
                     /locus_tag="J1Z28_002769"
     CDS             complement(5275..6738)
                     /gene="bepA"
                     /locus_tag="J1Z28_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBA7717591.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPIVVQSSEDFYL
                     AKVRTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            6951..8012
                     /locus_tag="J1Z28_002770"
     CDS             6951..8012
                     /locus_tag="J1Z28_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBA7717592.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(8111..8581)
                     /gene="bcp"
                     /locus_tag="J1Z28_002771"
     CDS             complement(8111..8581)
                     /gene="bcp"
                     /locus_tag="J1Z28_002771"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBA7717593.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(8581..9153)
                     /locus_tag="J1Z28_002772"
     CDS             complement(8581..9153)
                     /locus_tag="J1Z28_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBA7717594.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9299..10177
                     /gene="dapA"
                     /locus_tag="J1Z28_002773"
     CDS             9299..10177
                     /gene="dapA"
                     /locus_tag="J1Z28_002773"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBA7717595.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            10194..11228
                     /gene="bamC"
                     /locus_tag="J1Z28_002774"
     CDS             10194..11228
                     /gene="bamC"
                     /locus_tag="J1Z28_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBA7717596.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            11441..12154
                     /gene="purC"
                     /locus_tag="J1Z28_002775"
     CDS             11441..12154
                     /gene="purC"
                     /locus_tag="J1Z28_002775"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBA7717597.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            12322..13185
                     /locus_tag="J1Z28_002776"
     CDS             12322..13185
                     /locus_tag="J1Z28_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBA7717598.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            13200..15215
                     /locus_tag="J1Z28_002777"
     CDS             13200..15215
                     /locus_tag="J1Z28_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetyltransferase"
                     /protein_id="HBA7717599.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLYQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     IFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGAGRQLIVGALQYTHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLIRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVCTERLRDRITQWQFFH"
     gene            15289..15987
                     /gene="ypfH"
                     /locus_tag="J1Z28_002778"
     CDS             15289..15987
                     /gene="ypfH"
                     /locus_tag="J1Z28_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA7717600.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(16068..16268)
                     /locus_tag="J1Z28_002779"
     CDS             complement(16068..16268)
                     /locus_tag="J1Z28_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBA7717601.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(16296..17423)
                     /gene="dapE"
                     /locus_tag="J1Z28_002780"
     CDS             complement(16296..17423)
                     /gene="dapE"
                     /locus_tag="J1Z28_002780"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBA7717602.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(17427..17783)
                     /locus_tag="J1Z28_002781"
     CDS             complement(17427..17783)
                     /locus_tag="J1Z28_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBA7717603.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
BASE COUNT         4118 a         4511 c         4804 g         4460 t
ORIGIN      
        1 ccatccagcc acgcggcgtt ttcgtgcatt ttcagacgac catcggcaaa ccagctaatc
       61 accagtggat aaatagcatg ttcctgagtt tgcacgcggg cggtgatgtc atcttccgta
      121 tcaccagcaa ataccgggac tttcgcctgt aaaataaccg ggccaccgtc cagttcatcg
      181 gtgacgaaat gcaccgatgt accgtgctcc tcatcgccat tttccagcgc ctgacgatgg
      241 gtgtgtaatc cgggatattt cggcagcaga gaagggtgaa tgttcagcaa acgcccggca
      301 tagtgggaga caaacgccgg gctgagaatg cgcataaaac cagccagcac gaccacatcg
      361 ggtgcgtaca tgtcgatttc atgaatcaac tcccggtcat aggcttcacg actgtcaaac
      421 gcgctggtga tgagcgtatg cgttgcaata cccgcctggc gggcgcgttc aaggccgaac
      481 gcgtcggcct tattgctgaa aactgcccgt acggtgcctt taattttgtt ggttttgcag
      541 gcgtcaataa ttgcctgcaa attacttccg ttgccggaaa taagcaccac aatattcatt
      601 attcgataac cacgcgttgt tcggaatcag aggctttgat gataccgatt ttccacgcgt
      661 tttcaccgtt ggcattgagc agggcgaggg ctttgtccac ttccggagca ggcagggcaa
      721 taatcatacc gacgccgcag ttgaaggtgc gatacatttc atggcgctca acattacctg
      781 ccgtttgcag ccagttgaac acttccggcc actgccagga agattcatca atcactgcct
      841 gggtattatc tggcaatacg cgcggaatgt tttcccagaa gccgccgccg gtcaggtgcg
      901 caatggcatg cacatcgacc ttttcaatca actccagcac tgacttcacg taaatgcggg
      961 tcggtgccag cagatgatcg gctaatggct taccatcaag ttcggtggtt tgcggatcac
     1021 aaccgctgac ttcaagaatt ttgcgcacca gcgaatagcc gttcgagtgc ggaccgctgg
     1081 agccgagtgc aatcagcaca tcgccgtcgc tgactttaga tccgtcaatg atttctgatt
     1141 tttctaccac gccaacgcag aaaccagcga catcgtaatc ttcaccgtga tacatccccg
     1201 gcatttctgc cgtttcgcca cccaccagtg aacagcccga ttgcagacaa ccttccgcga
     1261 tgccgctgat caccgctgaa gcggtatcaa catccagttt tccggttgcg taatagtcga
     1321 ggaaaaacag cggttccgca ccttgcacca ccaggtcatt aacgcacatg gcgaccagat
     1381 caataccaat ggtgtcgtga cgttttaagt ccatcgccag acgcagcttg gtacctacgc
     1441 cgtcagtgcc ggaaaccagc acaggttcac gatatttttg cggcaatgca cacagcgcac
     1501 cgaagccgcc cagaccgccc atcacttccg gacgacgcgt tttcttcact acgcctttga
     1561 ttcttccaac cagagcatta cccgcgtcaa tatcaacacc ggcatctttg tagctaagag
     1621 aggttttatc ggtcactgct tgggtcccca cgcgttactt gcggtagaaa aataaaattc
     1681 gccgcaattc taacagggaa agcaaacgtt tgcgagactg ctttacacaa cctttttgta
     1741 cgtcttttcc ccaggcgcgc ggcaaaagaa tacttgtacc agggcaaagg atcgttttcg
     1801 gatggaataa tcttcttcca taaccatttg aatataaaat aactttatct caaaccgata
     1861 tcattttgac taaagtcaac gaaaataata ttgccgcctt gaagaaagga ggtataatcc
     1921 gtcgattttt ttgtggctgc ccctcaaagg agaaagagta tgaagatcgt ggaagtcaaa
     1981 cacccactcg tcaaacacaa gctgggactg atgcgtgagc aagatatcag caccaagcgc
     2041 tttcgcgaac tcgcttccga agtgggtagc ctgctgactt acgaagcgac cgccgacctc
     2101 gaaacggaaa aagtaactat cgaaggctgg aacggcccgg tagaaatcga tcagatcaaa
     2161 ggtaagaaaa ttaccgttgt gccaattctg cgtgccggtc tggggatgat ggacggtgtg
     2221 ctggaaaacg ttccgagcgc gcgtatcagc gtcgtcggta tgtaccgtaa tgaagaaacg
     2281 ctggagccgg taccgtactt ccagaaactg gtttctaaca tcgatgagcg tatggcgctg
     2341 atcgttgacc caatgctggc aaccggtggt tccgttatcg cgaccatcga cctgctgaaa
     2401 aaagcgggct gcagcagcat caaagttctg gtactggtcg ctgcgccaga aggtatcgct
     2461 gcgctggaaa aagcgcaccc ggacgtcgaa ctgtataccg catcgattga ccagggactg
     2521 aacgagcacg gatacattat tccgggcctc ggcgatgccg gtgacaaaat ctttggtacg
     2581 aaataaagaa taaaaataat taaagccgac tttaagagtc ggcttttttt tgagtaaagc
     2641 gcctataaca cataatacag aggataatac tatgacgcgc cgtgctatcg gggtgagtga
     2701 aagaccgcca cttttacaga caatcccgct tagtttgcaa catttgttcg ccatgtttgg
     2761 tgcaaccgtc ctggtgcccg tcttatttca tattaacccg gcgactgtac tgttatttaa
     2821 cggtatagga acactgctgt atctcttcat ctgtaaaggg aaaattccgg cttatcttgg
     2881 ttccagcttt gcctttattt caccggtatt gttactgttg ccgttagggt atgaagtcgc
     2941 gctgggcggc tttattatgt gcggcgtgct gttctgcctg gtttctttta tcgtgaagaa
     3001 agcggggacc ggctggctgg acgtgctgtt tccacctgcg gcaatgggcg caatcgttgc
     3061 cgtcatcggt ctggagctgg cgggcgtagc tgccggtatg gcgggtttac tcccggctga
     3121 agggcaaacg ccagactcca aaaccatcat catctctatt accaccctgg cggtcacggt
     3181 tttaggttcc gtgctgtttc gtggtttcct ggcaattatc ccgattttaa ttggcgtgct
     3241 ggtggggtac gcgctctctt tcgcaatggg aattgtcgat accacgccga ttattaatgc
     3301 tcactggttt gcgctgccga ccctctatac gccgcgcttc gagtggtttg ccattctgac
     3361 tattctgcca gcggcgttag tggttattgc cgaacacgta gggcacctgg tcgtaacggc
     3421 taatatcgtc aaaaaagatc tgctgcgcga tccaggtctg caccgttcga tgtttgctaa
     3481 cggcttgtcg accgtgatct ctggcttctt tggctccacg ccaaacacca catacggaga
     3541 aaacattggc gtgatggcga tcacccgtgt ttacagtacc tgggttatcg gcggtgcggc
     3601 gattttcgct atcctgcttt cctgcgtcgg taaactggct gctgctatcc agatgattcc
     3661 attgccggtg atgggcggcg tttcgctgct gctttacggt gtcatcggtg cttccggtat
     3721 tcgtgtgttg atcgaatcga aagtggatta caacaaagca cagaacctga tcctgacttc
     3781 cgttattttg atcatcggcg tcagtggcgc gaaggtaaac attggcgcgg cggagctgaa
     3841 aggtatggcg ctggcgacca tcgtcggtat cggcttaagt ttgatcttca aactgattag
     3901 tatgctgcgc cctgaagaag tggtgctgga cgcagaagac gcggatataa cagacaaata
     3961 atgtaccgac cgggcagtgt tgctgcccgg ttcaaacatc atgggattct gtggtaaact
     4021 tctcgcgatt ttgtgaaatc ctggttgagg tatctctgaa cacaccggca cagctctctt
     4081 tgccacttta tcttcctgac gacgaaacct ttgcaagttt ctggccgggg gataactcct
     4141 ctttactggc cgcgctgcaa aacgtgctgc gtcaggaaca tagcggttac atctatctct
     4201 gggcacgcga aggcgcgggg cgcagccatc tgctgcacgc ggcttgcgcg gaattgtcgc
     4261 agcgtggcga tgcggtgggc tatgtcccgc tggataaacg tacctggttt gttccggaag
     4321 tgctcgacgg tatggagcat ttgtcgctgg tctgtattga caacattgag tgtattgccg
     4381 gcgatgagtt gtgggagatg gcgattttcg atctctacaa tcgaattctg gaatcgggca
     4441 aaacacggtt gttgatcacc ggcgatcgtc caccgcggca gttgaatctg ggattaccgg
     4501 atctcgcgtc gcgtctcgac tgggggcaga tctacaaatt gcagccactt tctgatgaag
     4561 ataagttgca ggcgctacag ttacgcgcgc gtttgcgtgg ttttgaactg ccggaagatg
     4621 tggggcgttt cttgctgaag cggctcgaca gagaaatgcg cacgttgttt atgacgctgg
     4681 atcagctgga tcgtgcgtcg attaccgcgc aacgcaagct gaccattccg tttgtgaaag
     4741 aaattctgaa gttgtagata aggtgtttat tgtcggatgc ggcgcgaacg ccttatccga
     4801 cctacgcgaa gttaatccat agtattcgta ggccggataa gacgcggcaa gcgtcgcatc
     4861 cggcaatgtg cttcaactag cgatggagca catcttaacc cacgatttcc agtacctgct
     4921 ccggtggacg accaatccgc gctttgccat tcgcgaccac aatcgggcgt tccattagct
     4981 tcggattctc taccatcgcc tgaatcagca cctcttcgct aagcgaactg tcagccaggt
     5041 tcagttcttt ataaagatcc tctttttggc gcatcaattc acgagcgcta ttcatcccca
     5101 gcattttcag caaatcacgc agcgttgccg catcggcggg tgtctcaagg tagagcacca
     5161 cttctggctc cacgccgttt tctttcagca ggttcagcgt ttcccggctc tttgaacagc
     5221 gtgggttatg gtagattttt acctgtttgg tcattgttct tcctttaatg cgaattacat
     5281 cttggtataa ggcttaaagc gttcctgcag ctggcgcaac tggtcgatgc gcgcatcgta
     5341 acgcgcttgt tgcaggctgc ctaatttcac ctgcgaactg gcgctactca acaaggaaat
     5401 agcctgatcg agtcgcccgg cgagcgcata accttctgct cgcgcagcca gttcctgatc
     5461 gcggttattt agtgcggctt ccgcctgtgc cagtaaatcc cagccgttgc tgtcatcttt
     5521 attgttaaaa gtgtagcgat tcagaatatt ggccgcttcc tgtggttgcc ccccttgcag
     5581 ataagcgttc gccaggttga gctgcaacac cggattggtg cgcaaatcgc gggcattttt
     5641 caggcgattg atagcgtcat tggctttgtt ttgcccaaga tcgatatcgg tagccagatc
     5701 gagataccat gcgttgccag gttctgccgc cagtaacggt tgcagcgttt tacgcgcctc
     5761 gtcgtattta ttggcttcca tcgcctgtaa agcacgacca tattgcgccg ctcgttgctg
     5821 acgaacgttt cctttcgccc attcatccag caaatcactg gtgagttggt tacgccctga
     5881 attgtacatc cccagtgtgc gcacttttgc cagatagaaa tcttctgacg attgcaccac
     5941 tatcgggcgc atctgattgg cacggttacg ggcatcagcc agacggcttt ccggtagtgg
     6001 gtgagtgagc agaatttctg gcgggcgcga agagtaacgc gcctgatcga gtaatttttc
     6061 gaggaaggtc ggcatcgcct gcggatcgaa tcccgagcgt tgcagcacct gaataccaat
     6121 gcggtccgct tcctgttcat tttgctgggt gaaactgatc atcccctggc gcgttcccgc
     6181 cagtgtaccg gtcagcgccg ccatccccgc ctgcggactg gccatcgcca gtaaaataga
     6241 acctaacgcg ccgacccagg tcagcggcgc gctgcgctgc tgatcttcca tcgctcgcgc
     6301 caggtgacgt tgggtgacgt gggagatttc gtgcgccata actgaagcca gttgactttc
     6361 gttatcggaa taacggaaca gggcagagtg cagcaccacg ttgccgccaa agaaagcaaa
     6421 ggcgttaatt tcgtcgttgt tgatcagaaa gaaatgaaac ggtgtcttaa ccgaattggc
     6481 atgcgaaacc agacgcattc ctagcgaatt aatatattgc gttaatagcg ggtcattaat
     6541 taacggtgcg ctgccgcgta actggcggac ataatagtcg cccatctgca tttcctgacc
     6601 aatggaaagc gtgcttcctg cggaggttcc catatccggc aaggtgtctg cgctgtcggc
     6661 aaacgccggg gctacctgac caatggtcat agcagcaatg agggttgcaa ccaggttttt
     6721 tttcaactgc ctgaacataa cctctatcct gtatttctgg agatagtcat ttgaccgata
     6781 ctgtgaaata tcgttcccac cgcgccgcct ttgagtgtaa cggcgaagat tagcccactc
     6841 aaatgacttc tcagtttcct gaatccgtca taaaaagcga agtctttttt gtgacatttc
     6901 gatacaattg cggatcgcaa tcccgctctt gaggttcagg gaaggtctgt atgctcgaaa
     6961 tgttgatgca atggtatcgc cgccgtttta gcgacccgga agcgattgcc ttgctggtta
     7021 ttttggttgc tggttttggc attatctttt tctttagcgg cctactcgcg ccgttgctgg
     7081 tggctattgt gctggcctat ttgctggaat ggccgactgt ccgcctgcag tctattggct
     7141 gttcccgccc ttgggcgaca tctatcgtgc tgatcctgtt tgtgggcatc ctgctactga
     7201 tggcgttcgt ggttctgcct attgcctggc agcagggcat ctacttaatc cgcgatatgc
     7261 cagggatgct taataaactt tctgattttg ctgccacgtt accgcgccgg tatccggcat
     7321 taatggatgc ggggattatt gatgcaatgg ctgaaaatat gcgtagccgg atgctgacca
     7381 tgggcgattc ggtggtgaaa atttccctcg cctcgttggt cggtttgctg accattgccg
     7441 tctatctggt gctggtgcca ttgatggtct tcttcctgct gaaagacaaa gagcagatgc
     7501 tgaacgccgt tcgccgggtg ctgccgcgca accgtggact ggcaggacag gtgtggaagg
     7561 agatgaatca acaaatcacc aactatatcc gcggcaaagt gctggagatg atcgtggtag
     7621 ggatcgccac ctggctgggg ttcttgctct ttgggctgaa ctattcgctg ctgctggcgg
     7681 tgctggtcgg cttctcggta cttattccgt acattggcgc atttgtggtg accattccgg
     7741 tggttggcgt ggcgctattc cagtttggtg ctggcacgga attctggagc tgcttcgcgg
     7801 tgtatctgat tattcaggcg ctggacggca acctgttagt accggtgttg ttctccgaag
     7861 cggttaacct gcatcctctg gtgattattt tatcggtggt gatcttcggt ggtttgtggg
     7921 gattctgggg cgtattcttc gctattccat tggcgacgct gatcaaagcc gtgattcacg
     7981 cctggcctga tgggcaaatc gcgcaagact gaccttcagc gaaaaaattt taaacgagtg
     8041 ttgcagggca gtgagaagat ttgagatgtg ctggccgcaa gcgcagccag cacggaatgg
     8101 agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg tgattgctgg
     8161 ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg aagctgatgc
     8221 gatgaatacc atcgtaggtt ttgcccatga aggacttttc accccagacg ccgaattgtt
     8281 cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct ttttccgcaa
     8341 aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg cccgcttttt
     8401 tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg ggggtcatgg
     8461 ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa ttaacttgtt
     8521 ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc agtggattca
     8581 ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa tactgccttg
     8641 tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg cgtccgcaga
     8701 tgcggggctg tgggcagtta tctgaatatg caactgcgca gcccgttcat tttcagcagg
     8761 ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa gtgctgtgaa
     8821 gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag atgctggcat
     8881 tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca gctcggcacc
     8941 tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg aaagcagcat
     9001 gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat tacagccgca
     9061 actactgaca tgacgggtga tggtgttcac tattccaggg cgatcggcac ccaacgcagt
     9121 gatcaccaga taatgttgcg atgacagtgt caaactggtt attccttcaa ggggtgagtt
     9181 gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt ctgtctgctt
     9241 gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag gatggcccat
     9301 gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta atgtctgtcg
     9361 ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg cgatcgtttc
     9421 tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg atgtggtgat
     9481 gatgacgctg gagctggctg acgggcgcat tccggtgatt gccgggaccg gtgctaacgc
     9541 tactgcggaa gccattagcc tgacgcagcg cttcaatgac agcggtatcg tcggctgcct
     9601 gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc atttcaaagc
     9661 catcgctgaa catactgacc tgccgcaaat tctgtataat gtgccgtccc gtactggctg
     9721 cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta tcggaatcaa
     9781 agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt cagatgattt
     9841 tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag gcggtcatgg
     9901 ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt gcaaactggc
     9961 agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc cattacacaa
    10021 caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg aactgggtct
    10081 tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg gtcgtgagac
    10141 ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat ttgatggctt
    10201 actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt ttattactcg
    10261 ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa gcctacctgg
    10321 aagcggcacc gcttgcggag cttcatgccc cggctggaat gatcttgccg gtgacctccg
    10381 gcgattatgc aatcccggtg accaacggta gtggtgccgt cggtaaggcg ctggacattc
    10441 gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc acgggcgata
    10501 ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag gtggttagcg
    10561 tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag acactgacca
    10621 ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt cgttatcaaa
    10681 tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgctg aacctggaac
    10741 aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg gagatgatga
    10801 acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct gcgcaaaacc
    10861 gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta ccaatgctgg
    10921 tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agctgcgctg gaaaaagtgg
    10981 gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact tataagccgc
    11041 tgtctgacag cgaatggcac gaactgggcg cgagcgatcc aggtctggca tccggtgact
    11101 ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc gatccgaaag
    11161 gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag gctgcgttta
    11221 gcaagtaaaa atacagggct ggagtcatcc agcccttttt tctgatatga tacgcaaacg
    11281 tgtgcgtcga cagaaaaacg cgattttagc ggtaattcgc acgaaatttg tttgtcggac
    11341 gtaggtcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac cagacagcaa
    11401 aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc aagctgagtt
    11461 gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt tggtgctcga
    11521 attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg atcgcaaagg
    11581 tatggtgaac aacaagttca actacttcat tatgagcaaa ctggctgaag cgggtatccc
    11641 gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc tggatatggt
    11701 gccggttgag tgtgtcgtgc gtaaccgtgc tgctggctct ctggtgaaac gtcttggaat
    11761 cgaagaaggt attgagctga acccgccact gttcgatctg ttcctgaaaa acgacgccat
    11821 gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga gcaaagagaa
    11881 tctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa aactgttcga
    11941 tgatgcaggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca aaggcgaagt
    12001 ggttctgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag aaacgctgga
    12061 gaaaatggat aaagaccgtt tccgccagag cctcggtggc ctgatcgaag cctatgaagc
    12121 cgttgcccgc cgcctgggtg tacagctgga ctgatttttc tattcatcat cttgccgtgc
    12181 tactggcacg gcaagacaac cgctctcata agatgagtat tgaacgctca ttcctccgcc
    12241 atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca tttctataat
    12301 gaatataatg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga caatgttgaa
    12361 gacaggcgca atagctccgg cggtccgtca atgggcggtc ccggttttcg cctgccaagc
    12421 ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta tggcgttgat
    12481 ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacgcg gtcaattagc
    12541 cctaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac ggaagacacc
    12601 tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct ggtcatgtac
    12661 cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca taatggggcc attctattgc
    12721 ccggcggatg gcacagttta tatcgatctc tccttctatg atgacatgaa agacaaactt
    12781 ggcgcggatg gcgattttgc ccaggggtac gttatcgctc atgaagtcgg tcatcatgtg
    12841 cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc gacgcaggcg
    12901 gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc cggtgtctgg
    12961 gggcatagta tgcagcagca aggtgttctg gaaaccggcg atctggaaga ggcgctgaac
    13021 gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg agtagtacca
    13081 gacagtttca ctcatggcac ttctcagcaa cgctatagct ggtttaaacg tggtttcgac
    13141 agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc ggggcaggga
    13201 tggctgaact gactgcgctt cacacattaa cagcgcaaat gaaacgtgaa gggatccgcc
    13261 gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt aagttgcgtg
    13321 atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa aaccactgtt
    13381 caccctcggc gctacaaact ttacttgggc gcgagttccg gcatgcggta ttcgacgccc
    13441 gccacggctt tgatgccgct gcctttgcgg cacttagcgg aacgttgaaa gcgggaagtt
    13501 ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat gccgactcgc
    13561 tgcgctggag tgattgccct gaccctattg cgacgccgca ttttgtccag catttcaaac
    13621 gcgtacttac ggcgaataac gacgctatcc tctggcggca aaaccagccg ttctcgttgg
    13681 cgcattttac tccccgtact gactggcacc ccgctaccgg cgcaccacaa ccagaacaac
    13741 agcaactctt acagcagcta ctgaccatgc cgccgggtgt ggcagcggta actgctgcgc
    13801 gtgggcgcgg taagtcggcg ttggcagggc aactcatttc tcgtattgcg ggcagtgcga
    13861 ttgtcaccgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg ggcgagaagt
    13921 ttcgctttat tgcgccggat gccttgttag ccagcgatga gcaagccgac tggctggtgg
    13981 tcgatgaagc cgcagctata cctgcgccgt tgttgtatca actggtatcg cgttttcctc
    14041 gaacgttgtt aactactacg gtgcagggct acgaaggtac aggacgtggt tttttgctga
    14101 aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg atccgctggg
    14161 cacagggatg cccgctggaa aaaatggtta gtgaggcact gatttttgac gatgaaaact
    14221 tcacccacac accacaaggc aatatcgtca tttccgcatt tgaacaaacg ttatggcgaa
    14281 gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac taccggacct
    14341 cgccgctgga tttacgccgc atgatggatg caccaggtca acatttttta caggcggctg
    14401 gcgaaaacga gattgccgga gcgctatggc tggtggatga gggcggatta tctcaagaac
    14461 tcagtcaggc ggtatgggcg ggttttcgtc gcccgcgggg taatctggtg gcccagtcgc
    14521 tggcggcgca cggcagcaat ccactggcgg cgacattgcg cggacggcgg gttagccgga
    14581 tagcagttca tccggctcgt cagcgggaag gcgcagggcg acagcttatt gttggtgctt
    14641 tgcaatatac gcatgacctc gactatcttt cggtgagttt tggttacacc gaggagttat
    14701 ggcgtttctg gcaacgctgc ggttttgtgc tggtgcgaat gggtaatcat cgtgaagcca
    14761 gcagcggttg ctatacggcg atggcgctgt taccgatgag tgatgcgggt aaacagctgg
    14821 ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcgcag tggaatggcg
    14881 aaatgcttcc tgttgatcca ctaaacgatg ccgtcctttc tgacgacgac tggcttgaac
    14941 tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc ttaatacgtc
    15001 tgttacaaac cagcgaactg gcattaccgg cgctgcgtgg acgtttacag aaaaacgcca
    15061 gtgacgcgca gttatgtacc acacttaaac tttcaggccg taagctgtta ctggtccgtc
    15121 aacgcgaaga agccgcgcag gcgctattcg cacttgatga tgtttgcact gagcgtctgc
    15181 gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct cccatggtca
    15241 ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat gaaacatgac
    15301 cattttgttg ttcaaagccc ggataaacca gcacaacagt tgttactgct tttccacggt
    15361 gtcggggata acccggtggc aatgggggaa ataggtagct ggtttgcgcc gctgtttcct
    15421 gatgcgctgg tagtgagtgt cggtggcgcg gagccgagcg gtaatccggc ggggcgtcag
    15481 tggttttcgg tgcagggtat tacggaagat aatcgccagg cgcgtgtgga tgcgatcatg
    15541 ccgacgttta ttgagacggt gcgctactgg cagaaacaaa gcggagtagg ggctaatgcc
    15601 acggcgctaa tcggtttctc acaaggcgcg attatggcgc tggagagcat taaagccgaa
    15661 ccgggccttg cttcgcgcgt cattgccttc aacggacgct acgcgagcct gccggaaaca
    15721 gcgtctacag ccacaacaat tcacttgatt catggcggtg aagatccggt tatcgatctg
    15781 gcgcatgcgg ttgcggcgca ggaagcgtta atcagtgccg gtggcgatgt gacgctggat
    15841 atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaattagc cctggatcac
    15901 ttgcgttata ccattccgaa acattatttc gacgaagcgc taagtggcgg taagcctggc
    15961 gatgatgatg tgattgaaat gatgtaataa tagccgggta ggcctgataa gaccgtatca
    16021 ggcatcatgg ccggatgcgg cgttaacgcc ttatccggcc acttagctta cttcttcggt
    16081 tgatcctttt tcggccagtc gtcgtcatcg tcccatttat cgttgaaatc acgatgcggg
    16141 ggaagctccg gcttgttggc gagaaatttc ttatggtcga cgcgcttgag atctttaatc
    16201 acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata tttagccagc
    16261 cagtccatgc ttatttcctc ttgcagaacc actcatcagg cgacgagctg ttccatgata
    16321 cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc attaatttta
    16381 tgaatcgtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg ggcaataaag
    16441 cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt atagtgctca
    16501 accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg ctgcccggaa
    16561 agccaccaat ccaccgtata gcgcagttga tgtttttcaa gcagggcaag cacctgcgct
    16621 ttgatcatct catccgtcag ttcggtgcta aagcggaagt taaactgcac aaacagttca
    16681 cccggaataa cgttgttact gcccgttccc gcctgaatat tggcaatctg catactggtc
    16741 gccgggaaga attcattgcc ctgatcccac tcaatagcca ctaattcatt aaggaaaggt
    16801 gctgcgcgat gtaccggatt gtcagccaga tgtgggtagg caacatgccc ctgcacgcca
    16861 tgaatagtaa ggttacaggt taacgatccg cgacgaccat ttttcaccac atcaccaacc
    16921 acttcgatac tcgacggttc gccgaccagg cagtaatcga gacgctcatt acgcgccatt
    16981 aacgcttcga cgacttttac cgtaccgttg tgggcactgg cttcttcatc agaggtgatc
    17041 agaaatgcca gtcgtcccgt atggttggga tgttgtgcga caaagcgttc tgccgctacc
    17101 accatcgccg ccagcgagcc tttcatatct gccgcaccgc gcccgaataa catgccatca
    17161 cgaatggtgg gttcaaacgg cggattgatc caacggtcgg cgtcgccagg cggcaccacg
    17221 tcggtatgcc cggcaaaggc taacgtttca ccctgcccac gccatgccca aaaattctgc
    17281 gtgtcggcaa agtccatgcg ttcaacggta aaaccgatcg cctgcaaacg ttcaatcaac
    17341 aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag ctgttgtgtc
    17401 agctcaataa ccgggcacga catagactac acctcatgga aaaattgctg ataactggat
    17461 tcactgaaac ccagcagcat aggcttacca ggcgcgcaga gcaatggacg tttgataatt
    17521 gcaggcattt cagtcattaa tgtcgccgca gaggccgcat cggtgatttt attgcgggtg
    17581 gtttcgtcca gtttacgcca ggttgtacca cgggtgttga gtaacgcttc ccagcctaat
    17641 tcgttgataa aaccgttcaa taattcgctg tccaatccat cgacgcggta atcatgaaaa
    17701 cgatagtcga tgttattggc ttccagccaa cggcgagcct ttttaatggt gtcacaattt
    17761 ttgatgccgt aaagtgtaac cataatgaat cctttttcga tttatgagaa taaatactca
    17821 tttaagggtg aaaacatttt gcctgcaaat gatcatcagg aaatataaca ttatcgaagt
    17881 attcaaatta aca
//
LOCUS       DADSZD010000091        17889 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.091, whole genome shotgun sequence.
ACCESSION   DADSZD010000091 DADSZD010000000
VERSION     DADSZD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.091"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(1..747)
                     /locus_tag="J1Z28_002782"
                     /pseudo
     CDS             complement(1..747)
                     /locus_tag="J1Z28_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378101.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            810..1109
                     /locus_tag="J1Z28_002783"
     CDS             810..1109
                     /locus_tag="J1Z28_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7717604.1"
                     /translation="MLGVLTLARDIQLAIVNKMTQVLIGDIYLRQQCDVFWLGYTISP
                     AYTRQGYAIEAITATIDWIKENGFSLTKAGVNPENTLSKKLLIRIGFNFSSIEDE"
     gene            1486..1743
                     /locus_tag="J1Z28_002784"
     CDS             1486..1743
                     /locus_tag="J1Z28_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717605.1"
                     /translation="MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLT
                     DCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRAQLDNR"
     gene            1755..2306
                     /locus_tag="J1Z28_002785"
     CDS             1755..2306
                     /locus_tag="J1Z28_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016809596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7717606.1"
                     /translation="MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDES
                     ARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMHILAPLAVIPEYQGMGVGGRLIR
                     TGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
                     QPMTLTGHIRCAQALMKPEHWRE"
     gene            2358..3104
                     /locus_tag="J1Z28_002786"
                     /pseudo
     CDS             2358..3104
                     /locus_tag="J1Z28_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418726.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            3231..3491
                     /locus_tag="J1Z28_002787"
     CDS             3231..3491
                     /locus_tag="J1Z28_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717607.1"
                     /translation="MSQYEQFCQACGMPMSAPDAHSASDQYCAYCSDSNGNLKPWEEA
                     VSGLASFLDSWQKVGPEEAHKRAKRYLTAMPAWAHKAESDLS"
     gene            3890..5011
                     /locus_tag="J1Z28_002788"
     CDS             3890..5011
                     /locus_tag="J1Z28_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBA7717608.1"
                     /translation="MSFSVQETLFSLLQHNAISGHENAVADVMLCEFRRQAKEVWRDR
                     LGNVVARYGSDKPDALRLMIFAHMDEVGFMVRKIEPSGFLRFERVGGPAQVTMAGSIV
                     TLTGDKGPVMGCIGIKSYHFAKGDERTQSPSVDKLWIDIGAKDKDDAIRMGIQVGTPV
                     TLYNPPQLLANDLVCSKALDDRLGCTALLGVADAISTTELDIAVYLVASVQEEFNIRG
                     IVPVLRRVKPDLAIGIDITPSCDTPDLHDYSEVRINQGVGITCLNYHGRGTLAGLITP
                     PRLIRMLEQTALEHNIPVQREVAPGVITETGYIQVEQDGIPCASLSIPCRYTHSPAEV
                     ASLRDLTDCIRLLTALAGMSAAHFPVEPDSGTTQEAHPL"
     gene            5008..5286
                     /locus_tag="J1Z28_002789"
     CDS             5008..5286
                     /locus_tag="J1Z28_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA7717609.1"
                     /translation="MKKILVACGTGMSTSTMIAHKLQEFLTEQGISATTAQCCLNEIP
                     LNCNGMDLIVTSMRTNSDYGIPTLNGAALLTGINDDALKQQIKALLTQ"
     gene            5298..6611
                     /locus_tag="J1Z28_002790"
     CDS             5298..6611
                     /locus_tag="J1Z28_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBA7717610.1"
                     /translation="MFDYILSLGGTVFVPIIMIVIGLIFRIPWLQAIKAGVTVGIGFV
                     GMGLVIVMAIDSLSPPIKVMIERFGLALHVFDVGAGPASGVGYATAIGAMIIPVIFLL
                     NVTMLVTRLTKTMNVDIYNYWHYAITGTVVQLMTGSLIYGVLGAICHAALSLKMADWT
                     AKRVQNIVGLEGISIPQGYGSSSVPLFVLLDAIYEKIPFMKGRNIDAQEIQKRYGMVG
                     DPVIIGVVLGLIFGLAAGEGFKGCASLMITVAAIMVLFPRMIRLIVEGLMPISDGARK
                     FFQKYFKGREVYIGLDTAVTLGHPTTIAVGLLLIPIMLILASILPGNKVLPLADLPVA
                     PFFICMATVIHRGDLVRTLISGVIVMITVLLIATQFAPYFTEMALKGDFSFAGESAQI
                     SALSVGNMFGWSISELMSLGIIGVVVAVGIVASVVLFLRKRELSE"
     gene            6624..7430
                     /gene="sgcQ"
                     /locus_tag="J1Z28_002791"
     CDS             6624..7430
                     /gene="sgcQ"
                     /locus_tag="J1Z28_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein SgcQ"
                     /protein_id="HBA7717611.1"
                     /translation="MSWLKEVIGTEKAVIAMCHLRALPGDPSFDAQLGMNWVIDKAWD
                     DLMALQNGGVDAVMFSNEFSLPYLTKVRPETTAAMARIIGQLMSDIRIPFGVNVLWDP
                     VASFDLAMATGAKFIREIFTGAYASDFGVWDTNVGETIRHQHRIGAGEVKTLFNIVPE
                     AAVYLGNRDICSIAKSTVFNNHPDALCVSGLTAGTRTDSALLKRVKETVPDTVVLANT
                     GVCLENVEEQLSIADGCVTATTFKKDGVFANFVDQARVSQFMEKVHHIRR"
     gene            7561..7992
                     /locus_tag="J1Z28_002792"
     CDS             7561..7992
                     /locus_tag="J1Z28_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7717612.1"
                     /translation="MINDIKWVQAQREATDWRQAVEIATRPLVAYGAAQPCYVNGIIE
                     NTLNWGPYYLIAPGIALPHARPEQGANYNQVSITTLRTPVAFGNEECDPVWLLLCVSA
                     TDANAHILTIQRISQFIDSPQRLAAVGNASTDDALFALVSG"
     gene            8004..8636
                     /locus_tag="J1Z28_002793"
     CDS             8004..8636
                     /locus_tag="J1Z28_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3 epimerase family protein"
                     /protein_id="HBA7717613.1"
                     /translation="MILHPSLASANPLHYGRELTALDNLDFGSLHLDIEDSSFINNIT
                     FGMKTVQAVARQTPHPLSFHFMVARPQRWFNALAEIRPAWIFVHAETLDYPSETLTEI
                     RHTGARAGLVFNPATPIDAWRYLASELDGVMVMTSEPDGQGQRFIPSMCEKIQKVRTA
                     FPQTECWADGGITLAAAQQLAAAGAQHMVIGRALFSSSDYRATLAQFATL"
     gene            8653..9435
                     /locus_tag="J1Z28_002794"
     CDS             8653..9435
                     /locus_tag="J1Z28_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7717614.1"
                     /translation="MSQQRPDRIKQMLHYLWQHRHLSTQQAMELFGYAEATVRRDFQY
                     IANQYPGMIRGHGCLDFDDSTDDKEYVFDVKRTLQSVAKREIAALARTMIKDGDCFFL
                     DSGSTCLELAKCLADARVKVICNDIKIANELGCFPHVESYIIGGLIRPGYFSVGESLA
                     LEMINAFSVERAFISCDALSLETGITNATMFEVGVKTRIIQRSREVILMADHSKFDAV
                     EPHAVATLSCIKTIISDSGLPETIAQRYQRAGCQLFLPHSIK"
     gene            9738..10526
                     /locus_tag="J1Z28_002795"
     CDS             9738..10526
                     /locus_tag="J1Z28_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA7717615.1"
                     /translation="MVRKGCNSLVRAEKILTHIAWVGMASYMELLNKFQYPKSSLLNL
                     LNVMVECGFLIKNKNGYYSLGIKNYELGCQALHRQNIFEVTKRPMQELSLKSGLVCHL
                     GAMESISAIYLDKIESPDSVPTSKSWIGKKLELHITALGKALLAWKTREELDYFLEAL
                     TLTPHTRNTFTDKKLFLEELQKTRLRGWAIDNEESTYGAVCLSMPVFNMYNRVNYAIS
                     LSGDPVVYSGNKIDSYLELLRKCAEQISYGLGYRNENEHLRKGN"
     gene            10531..11436
                     /locus_tag="J1Z28_002796"
     CDS             10531..11436
                     /locus_tag="J1Z28_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBA7717616.1"
                     /translation="MKKFSGIIPPVSSTFHRDGTLDKKAMREVADFLINKGVDGLFYL
                     GTGGEFSQMNTAQRMALAEEAVTIVDGRVPVLIGVGSPSTDEAVKLAQHAQAYGADGI
                     VAINPYYWKVAPRNLDDYYQQIARSVTLPVILYNFPDLTGQDLTPETVTRLALQNENI
                     VGIKDTIDSVGHLRTMINTVKSVRPSFSVFCGYDDHLLNTMLLGGDGAITASANFAPE
                     LSVGIYRAWREGDLATAATLNKKLLQLPAIYALETPFVSLIKYSMQCVGLPVETYCLP
                     PILEASEEAKDKVHVLLTAQGILPV"
     gene            11447..13414
                     /locus_tag="J1Z28_002797"
     CDS             11447..13414
                     /locus_tag="J1Z28_002797"
                     /EC_number="4.2.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjhG/YagF family D-xylonate dehydratase"
                     /protein_id="HBA7717617.1"
                     /translation="MSVRNIFADESHDIYTVRTHADGPDGELPLTAEMLINRPSGDLF
                     GMTMNAGMGWSPDELDRDGILLLSTLGGLRGADGKPVALALHQGHYELDIQMKAAAEV
                     IKANHALPYAVYVSDPCDGRTQGTTGMFDSLPYRNDASMVMRRLIRSLPDAKAVIGVA
                     SCDKGLPATMMALAAQHNIATVLVPGGATLPAKDGEDNGKVQTIGARFANGELSLQDA
                     RRAGCKACASSGGGCQFLGTAGTSQVVAEGLGLAIPHSALAPSGEPVWREIARASARA
                     ALNLSQKGITTREILTDKAIENAMTVHAAFGGSTNLLLHIPAIAHQAGCHIPTVDDWI
                     RINKRVPRLVSVLPNGPVYHPTVNAFMAGGVPEVMLHLRSLGLLHEDVMTVTGSTLKE
                     NLDWWEHSERRQRFKQLLLDQEQINADEVIMSPQQAKARGLTSTITFPVGNIAPEGSV
                     IKSTAIDPSMIDEQGIYYHKGVAKVYLSEKSAIYDIKHDKIKAGDILVIIGVGPSGTG
                     MEETYQVTSALKHLSYGKHVSLITDARFSGVSTGACIGHVGPEALAGGPIGKLRTGDV
                     IEIKIDCRELHGEVNFLGTRSDEQLPSQEEATAILNARPSHQDLLPDPELPDDTQLWA
                     MLQAVSGGTWTGCIYDVNKIGAALRDFMNKN"
     gene            13521..14870
                     /locus_tag="J1Z28_002798"
     CDS             13521..14870
                     /locus_tag="J1Z28_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HBA7717618.1"
                     /translation="MPLIIVVAGIALLLLLTIKIKLNTFVSLIIVSIAVAIASGMDLS
                     KVVTSVESGLGGTLGHIGLIFGFGVMLGRLLADAGGAQRIALTMLNYFGKNKLDWAVV
                     CSAFIVGIALFFEVGLILLVPILFAIAREAKISPMFMCVPMLSGLLVAHGFLPPHPGP
                     TVIAREYGADVGLVLIYGIIVGIPTFILCGPVLNKFCQRIIPDAFKKEGNIASLGATR
                     RFSESEMPGFGISFLTAMLPVILMAVVTIIQMTHAKSAADSGLFYNVILFLGNSTIAM
                     LISLLFAIYTMGLGRGKTIPDLMDSCGKAIAGIAGLLLIIGGGGAFKQVLIDSGVGQY
                     ISTLVSGMDINPILMAWGVAAFLRICLGSATVAAISTAGLVIPLLAVHPNTNLALITL
                     ATGAGSCICSHVNDASFWMIKDFFGLTTKETLLSWTLMSTLLSISGLIFILLASLVL"
     gene            15217..16203
                     /locus_tag="J1Z28_002799"
     CDS             15217..16203
                     /locus_tag="J1Z28_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBA7717619.1"
                     /translation="MDRDQTNSSLFNDDPVLHATWLYYQEGKSQTEVAAIMGVSRVTV
                     VKYLQTARENGLVHINLDVNVFGSIDAALQIRDKFNLQRVIIVPDGEHAGKRDDTKLM
                     RTRLSRAGGMYLNQVIENGDVLGVAWGRTIHQMSKTMTPKSCKNVTVIQMLGSMPSQP
                     DLTIIESSSQIAYKLSGRIASLHVPAVVSSARLAMELQAEPIIRSNFDVLTRCTKAFF
                     VVGNALDENPLIRVGVLNKKEMQTYRDLGAVGVICGRFYDKEGMPVVADVDQRILGIS
                     LAQLRQIERKIFLAGGERGYDATLGALLGGYVTDLIVDEGTAEFLLACELPH"
     gene            complement(16244..17248)
                     /locus_tag="J1Z28_002800"
     CDS             complement(16244..17248)
                     /locus_tag="J1Z28_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HBA7717620.1"
                     /translation="MFIVESYAVAIIMCFITMICWGSWANTTKLVSNKKWEFPLFYWD
                     YSIGLLLCSLLFAFTRGSMGEAGRSFIPDIQQASSSSLMSAILAGIIFNISNILLVAS
                     INLAGMAVAFPVGVGLALPLGVITTYIGNPQGDPLILFLGVACVVIAIIFTAIAYGRV
                     TQEADKSRRNKGLITAILAGIIMGWFFRFLADSMSDNFSQPASGLMTPYSALVLFAVG
                     LFLSNFVLNTLVMKKPISGEPVNGKMYFSGSLRDHVCGWLGGMIWCVGLAFSLIASGQ
                     AGYAISYGLGQGATMIAVIWGVFIWREFASAPAGTNKLLLTMFISYIVGIVLIIAANQ
                     "
     gene            complement(17352..17717)
                     /locus_tag="J1Z28_002801"
     CDS             complement(17352..17717)
                     /locus_tag="J1Z28_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fuculose phosphate aldolase"
                     /protein_id="HBA7717621.1"
                     /translation="MYGRYRAARLPSPSGRHCSHYMVAVSGTDHIPCIPYYTFGSPEL
                     ADGVSKGIRESKSLLMQHHGMLAMDVTLEKTLWLAGETETLADLYIKCGGLHHDVPVL
                     SEAEMTIVLEKFKTYGLKA"
     gene            complement(17659..17739)
                     /locus_tag="J1Z28_002802"
                     /pseudo
     CDS             complement(17659..17739)
                     /locus_tag="J1Z28_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4472 a         4449 c         4540 g         4428 t
ORIGIN      
        1 aattaaatcc tgtcgtttct gaaactcaaa ttgcaacgca ttattatctg aagaaaagag
       61 atattttgcc aataaccctc gcggcgaaaa tgcgggctgc atctcgataa ggtccatatc
      121 gccaaatacc agaaaactat ctttagcgcg tgagacagca acattgagga tactgctgtt
      181 gctatcaaga aacctgccat cttcatgttt tgagtagaca ggagaaaaga gaacaatcgc
      241 cctttccgcc ccctgcaagg agtgcacagt ccccactgtc agtgaaccct gctcgtcttt
      301 gccattaatt tccagcttac gcaatgacgt tttgatagca ttaacctgcg ccgaaaatgg
      361 cgtcacaaca ccaacaactt tgtacagtgg ttccccgtaa tggcgctcta tatcatcctt
      421 atgtgccacc agccatgctg ctattgtttc agcttcaaga gggttataac ggctccctcc
      481 atttggcttc atgcctctac catcaatttg taaatatccc attgcgggga aaatcgtccc
      541 tttttccatc cctcttttag gctgcaactt accgtgatag caaagtgagt tacagtagcc
      601 gatgatgtta ttgaaacagc gacgatgctc atacagatac attccgcgct cgaacttggg
      661 atcatactgg tagtgagagt tgaactgggc tacctacatt acgctgccag atgctgcact
      721 ttttccagac ccatttatta tcgaatacgg tgaccgcctg agcggccacc tttaatcgtg
      781 ggcagttaca cagaattatt tacagggcct tgctgggagt tcttacactt gctcgcgata
      841 ttcaattggc cattgtcaac aagatgacgc aggttttgat tggtgatatc tatttacgtc
      901 agcaatgcga tgtgttttgg ctcggctata ccatcagtcc cgcttatact cgccagggat
      961 atgcaattga agcaattacc gccacgattg actggataaa agagaacggt ttttcactca
     1021 ccaaagccgg tgtgaatccg gaaaataccc tttcaaaaaa attacttatc aggattggct
     1081 tcaatttttc gagcatcgaa gatgagtgag catatttatg ttctggatta aacaaaagga
     1141 gaataatctc ccgaagggaa aagccgatga ggccatttca ggcgtacttc aacatgcatt
     1201 cgtcagtgtc atctgccttt tccagtaaat gggttggcat tcaatttcca aagtacatca
     1261 cactttttct actgaaacat atccctccta taactacgtg ttaatattcg ctccgtaaat
     1321 acctttcaac tcaacccgaa gctttgatgc tggagaatgt aatcatccga taatcagttc
     1381 cccgaccttt tcaggccgga ctgattatca atgcgccgaa atcgaatgcg gacaccgcgg
     1441 tgtgtttgca cgttttgcac atgatgatta aataggttct tcagtatgaa tttatcccgt
     1501 caggaacaac ataccttaca cgttctcgct aaaggtagac gtattgcgca cgtccgcgat
     1561 tcttcaggcc gcgtcacttc cgttgaatgc tacagccgcg aagggctgtt gctgaccgac
     1621 tgcacgctcg ccgtcttcaa aaaactcaaa accaaaaaac ttatcaagtc cgtcaatggc
     1681 cagccctatc gaatcaacac caccgagctg aataaagttc gcgctcagct cgataatcgc
     1741 taaggagagt ttctatgaca gtacaccact tcacttttca tatcacagat aaaagtgatg
     1801 ccagtgatat ccgggaagtc gaaacccgcg catttggctt cagcaaagaa gccgatttag
     1861 tggcctcttt acttgaggat gaaagcgcgc gccccgcgtt atcgctgcta gcccggtatg
     1921 aaggcaaagc cgtcgggcat attttattta cccgggcgac cttcaaagga gaaatggatt
     1981 caccgctgat gcacatcctc gctcccctgg cggtcatacc ggaataccaa gggatgggcg
     2041 tgggaggacg gttgatacgc actggcatcg agcacctgag gttgatgggc tgccagacgg
     2101 tttttgttct cggccacgcc acctattacc cccgacatgg gttcgaaccc tgtgccggag
     2161 acaaaggcta tccggcacct tatcccatcc cggaggagca caaagcctgc tggatgatgc
     2221 aatcactcac cgctcagccc atgaccctga ccggccacat ccggtgcgcg caggccctga
     2281 tgaaaccgga gcactggcgt gaataacaat cttgttgtgt cctttttaag gcttaaccca
     2341 ataacaggaa ttaaataatg gatatcccac gtatttttac catcagcgaa agtgaacacc
     2401 gcatccataa cccgttcacc gaagagaagt acgccacgct gggtcgcgtg ctgcgcatga
     2461 agccgggcac ccgcattctt gacctcggca gcggctcggg cgagatgctc tgcacctagg
     2521 ccagggatca tggcattacg gggactggca tcgacatgag ttcgctcttc accgcgcagg
     2581 caaagcgtcg cgcggaagaa ctcggcgtca gcgaacgcgt acattttatt cataacgatg
     2641 cggccggtta cgtcgcaaat gaaaaatgtg acgtggcggc ctgcgtcggc gcgacatgga
     2701 ttgccggtgg gtttgccggg gcggaggagt tgctagcgca aagcctgaaa ccgggcggaa
     2761 tcatgcttat cggcgaaccc tactggcgtc agctaccagc aacagaagaa atagcccaag
     2821 cctgcggcgt cagctcgaca agcgatttcc tgacgctgcc aggacttgtc ggagcgttcg
     2881 acgatctcgg ctatgacgtg gtggaaatgg tactggcaga ccaggaaggc tgggaccggt
     2941 atgaagccgc aaaatggctg accatgcgcc gctggctgga agcgaatcct gacgatgact
     3001 tcgccgccga agtcagggcc gaactaaaca tcgcgccgaa acgctacgtg acctacgcgc
     3061 gggaatgctt tggctggggc gtgttcgcgc tcatcgccag gtaacaacag gttcccggta
     3121 tttctggctg gaaatatcga ggctttacct ttgctttgat ctgatgtatc acctgcacaa
     3181 tacatattca gtatgtttat taatcaagat gtcaccataa ggagcatcat atgtcacaat
     3241 atgaacagtt ctgtcaggct tgcggaatgc cgatgtcggc accagatgca cacagtgcca
     3301 gtgatcaata ttgtgcgtac tgcagcgaca gcaacggcaa tctcaaacct tgggaagaag
     3361 cggtatcagg tttagccagc ttccttgatt cctggcaaaa agtcggcccg gaggaagcac
     3421 ataagcgtgc aaaacgttat ctgacggcaa tgccagcctg ggctcataaa gcggagagtg
     3481 atttgagctg attatctata ctccctctga atctattcag gagatccgat gttttcttac
     3541 attatgctgg gaaccaacga cctgccacgc gccattacat tctaagatcc gctgatggag
     3601 atgctggggt atccacgtgc aggacgtaat gaagagggag cgtcctgcaa caaagtggtg
     3661 cgataacagt taccgcgttt tattcatcct cattatttca accataatca ctggtctctc
     3721 cagcccgtaa ctttcacaaa cgttgagcgc ttttattcca ctcggctcac ataatttttt
     3781 atttgttcag ctttgcgcgt cttcacgtga acgttctcac aattattcga ccagtctgcg
     3841 cctataaaac gctcaacgag tgattgtctt acggtctacg gagatagcta tgtcattttc
     3901 tgtgcaggaa accctgttct cgcttttgca gcacaatgcg atttcaggac acgaaaacgc
     3961 tgtcgctgac gtcatgctgt gcgaattcag gcgtcaggca aaagaggtct ggcgagacag
     4021 gcttgggaat gtcgtcgcgc gctacggtag tgataaaccc gatgcgctgc gactgatgat
     4081 ttttgcgcat atggatgaag tcggttttat ggtgcgcaaa atagagccgt ctggattttt
     4141 acgctttgaa cgcgtaggcg gtcctgcgca ggtcactatg gcaggttcca tcgtcaccct
     4201 caccggggac aaagggccag tcatggggtg tatcggcatt aagtcctacc actttgccaa
     4261 aggcgacgag cgcacgcagt caccttctgt cgacaaactg tggattgata ttggtgccaa
     4321 agacaaagac gacgctatac ggatgggcat tcaggtcggt acgcctgtca ctctgtataa
     4381 cccgccgcaa ctcctggcaa acgatctggt gtgcagtaaa gcactagacg atcgtctggg
     4441 ctgtactgcc ctgctcggtg tagcggatgc tatcagtact acggagcttg atatcgccgt
     4501 ttatctggtg gcttcggtac aggaagaatt taatatccgc ggcattgttc ccgtattacg
     4561 ccgtgtaaaa cctgacctgg cgattggtat tgatatcact ccatcgtgtg acacccctga
     4621 tttacacgat tattccgagg tcaggattaa tcagggcgtt gggatcacct gcctgaacta
     4681 ccatggtcgg ggaacgctgg ccggattaat cacgcctcct cgcctgatac ggatgttgga
     4741 acagacggct cttgaacaca acattccggt gcagcgagaa gtggctcccg gcgtgataac
     4801 agaaaccggt tatatccagg ttgagcagga tggtattccc tgcgccagtc tctctattcc
     4861 ttgtcgctat acccattctc cggcggaggt tgccagcctg cgtgatttga ctgattgcat
     4921 ccgcctattg accgctctgg caggtatgtc agcagcacat ttccccgttg agcctgattc
     4981 aggcactaca caagaggcac atccattatg aaaaagatcc ttgtggcatg cggtaccggc
     5041 atgtcgactt caaccatgat tgcacacaag ctgcaagagt tcctgactga acagggcatt
     5101 tcagccacta ccgcccaatg ctgtctgaat gagatccctc ttaactgtaa cggtatggac
     5161 cttattgtaa cctccatgcg aaccaacagt gattacggca tccctacgct taatggcgcc
     5221 gccctgctca cagggatcaa cgacgacgcg ttaaaacaac aaatcaaggc tctgttaact
     5281 caataaaagg aggcattatg tttgattaca tcctgtctct cggcggtacc gttttcgtcc
     5341 ctatcatcat gatcgtgatt ggccttatct tccgcatccc ctggctgcag gcgattaagg
     5401 caggggtaac ggttgggatt ggttttgtcg gtatgggact ggtgatcgtc atggcgatcg
     5461 acagcctcag tccccctatc aaagtgatga ttgagcgttt cggcctggcc ctgcatgtct
     5521 ttgacgttgg cgcaggccct gcctccggcg ttggttatgc gacagccatt ggcgccatga
     5581 ttatcccagt tatttttctt ctcaatgtca ccatgctggt cacccgtctg accaaaacga
     5641 tgaacgtcga tatttacaat tactggcact acgccattac gggtacggtg gtccagctta
     5701 tgaccggtag cctgatttat ggtgtgctgg gggcaatctg ccatgcggcg ctatcgttaa
     5761 aaatggccga ctggacggca aaacgggtac agaacatcgt cgggctggaa ggtatttcga
     5821 taccgcaagg ctacggttcc agttcagtac cgcttttcgt tctacttgat gctatctatg
     5881 aaaaaatccc tttcatgaag ggacgcaata tcgatgctca ggagatccag aaacgctacg
     5941 gcatggtcgg cgatccggtg attatcggcg tagtgctcgg tctgattttt ggcctggcgg
     6001 ctggtgaagg ctttaaaggt tgcgccagtc tgatgattac cgttgcggca atcatggtgc
     6061 tgttcccgcg catgatccgc ctgattgttg agggcctgat gcccatctcc gacggtgcaa
     6121 gaaagttctt ccagaaatat ttcaaaggac gtgaagtgta tatcggtctg gatactgctg
     6181 tgacgctggg gcatccaacc accattgctg tcggtctgct actgataccc attatgttaa
     6241 ttctggccag cattttaccg ggcaacaaag tgttacccct cgcagacctg ccggtagcac
     6301 cgttctttat ctgtatggct acggttatcc accgcggaga cctggtcagg acgttgataa
     6361 gcggggtcat cgtgatgatt accgtcctgc tgatcgccac ccagttcgcg ccttacttca
     6421 ctgaaatggc gctcaaaggc gatttcagtt ttgccggtga aagtgcgcaa atctcagcgt
     6481 tgtccgtggg caatatgttc ggttggtcga tttctgaatt gatgtcgctt gggattattg
     6541 gcgttgtcgt ggcggtcggt attgtcgcca gcgtcgtgct gttcttacgt aaacgtgaat
     6601 tatcggaata aggagtactt tcaatgagtt ggctgaaaga ggttattggg acggaaaaag
     6661 cggtcatcgc catgtgccat ctacgtgcat tgcccggcga cccgagtttt gatgcgcagt
     6721 tggggatgaa ctgggttatc gacaaagcct gggacgatct gatggcgctc caaaatggtg
     6781 gggtggatgc agtcatgttc tccaacgagt ttagtcttcc ctatctcacg aaggtgcgtc
     6841 cggaaaccac tgcggcaatg gcacgaatca tcgggcaatt aatgagcgat atccgcatcc
     6901 cgtttggcgt gaatgttctg tgggatccgg tcgcctcatt cgacctggcg atggctaccg
     6961 gcgctaagtt tatccgcgag atatttaccg gtgcctatgc cagcgacttt ggcgtctggg
     7021 acactaacgt cggtgagacc atccgtcatc aacaccggat tggtgcaggc gaagtgaaaa
     7081 ccttgttcaa tatcgtccca gaggccgccg tgtatctggg caatcgcgat atctgttcta
     7141 ttgctaaatc gacagtattt aacaaccatc ctgatgcgct ctgtgtttct ggcctgaccg
     7201 ccgggacgcg tacagacagc gcgctgttaa aacgggtcaa agaaacggtg cctgacaccg
     7261 tcgtgttggc caacacgggc gtctgcctgg aaaacgtgga ggaacaactc agtatcgccg
     7321 atggctgtgt gaccgcaacc acctttaaaa aggacggcgt ttttgccaat ttcgttgacc
     7381 aggcgcgagt cagtcagttt atggagaaag tgcatcatat acgccgataa gatggggata
     7441 agcagagcgc ttatccccgg caaagagcaa taacccttct gcgatccctg ccgccttcgt
     7501 caaaagtatc gacggggatc gctaccagat taacccgaat cggcatggag tcaacgacgg
     7561 atgattaacg atattaaatg ggtacaggca caacgtgaag ccaccgactg gcgtcaggcc
     7621 gtggaaatcg ccacccgccc gctggtagcc tatggtgccg cgcagccatg ctacgttaat
     7681 gggatcatcg aaaataccct taactggggg ccttattatc tgatagcccc aggaatcgcc
     7741 ttgcctcacg cccgaccaga gcaaggcgca aactataatc aggtcagtat caccacgcta
     7801 cgcaccccgg tcgcctttgg taacgaggag tgcgaccccg tctggctact tctctgcgtc
     7861 agcgccacgg atgccaacgc acatattttg accattcagc gcatcagcca gtttattgat
     7921 tcgccacaac gcttagcggc agtgggaaat gccagcacag acgatgcact gtttgcgctg
     7981 gtatcaggtt aacggaggcg cttatgatcc tgcacccttc tctcgccagc gcaaatcctc
     8041 tgcactatgg tcgtgagctg acggcattgg acaatctgga ttttggaagc ctgcatctgg
     8101 atattgagga cagcagcttc attaacaaca ttacgtttgg gatgaaaacg gtgcaggccg
     8161 tcgccagaca gacgcctcat cccttgtcct ttcatttcat ggttgcccgg ccacagcgct
     8221 ggtttaacgc gctggctgaa atcagacccg cgtggatctt cgttcatgca gaaacgctgg
     8281 actatccttc ggaaacgtta actgagatcc gccataccgg cgcacgagcc ggactggtat
     8341 ttaatccggc aaccccgatc gacgcctggc gatatctggc gtctgagctg gacggagtga
     8401 tggtgatgac cagtgagccc gatgggcagg gacagcggtt tattccctcc atgtgcgaga
     8461 aaatccaaaa agtacgcacg gcttttcctc agaccgagtg ctgggccgac ggtggaatta
     8521 cgttggcagc tgcacagcaa ctggcagcag ccggggcgca acatatggtg attgggcgcg
     8581 ccctgttctc ttcatctgac tatcgcgcga ccttagctca attcgccacc ctgtaagagg
     8641 agattgtttc tgatgagcca acagagacct gaccgtatca aacagatgct gcactacctg
     8701 tggcaacatc gtcacctctc cacacagcag gccatggagc tttttggcta tgcggaagcg
     8761 actgtccgtc gtgatttcca gtatattgcc aaccagtatc caggcatgat ccgcggtcac
     8821 ggttgcctgg acttcgacga cagtacagac gacaaagagt acgtcttcga cgtgaaacgt
     8881 acattgcaat ctgtggcgaa aagagaaata gctgcccttg cccgaacaat gatcaaagat
     8941 ggcgactgct tttttctcga ctccggctca acctgcctcg aactggcgaa atgcctggcc
     9001 gatgcgcggg taaaggtcat ctgcaacgac attaaaatcg ccaatgaact gggctgtttt
     9061 ccccatgtgg aaagttacat tatcggagga ctgattcgcc caggttattt ttcggtagga
     9121 gagagtctgg cgctggagat gatcaatgcc ttttccgtag aacgtgcgtt tatctcctgc
     9181 gatgcgctgt cgctggagac ggggatcacc aatgccacca tgtttgaggt cggggtgaaa
     9241 acacggatta ttcaacgctc gcgtgaagtt attctcatgg ccgatcactc aaaatttgat
     9301 gccgttgagc cgcatgctgt agcaacacta tcatgtatta aaacgatcat cagtgacagc
     9361 gggttacctg agactattgc acagcgttat cagcgagccg gatgtcagtt gtttttacct
     9421 cattcaataa agtgataagt aagttcgttc gagagggatt tcaagcaaaa ataatcaatg
     9481 gcacccaata gaaaatattg gcgatgcgct cgaacgaata aagaagccct atgcgcaatc
     9541 cacacgctac gatgttgcaa caacacgcca tctacttttt attctcattc actaaatgtg
     9601 gttgttctgg attcattatt caaagtgtgt acaagatcac atttaatcac ttcattacgg
     9661 ttcagcatgc tgaacaaagc atattttcca ctatgtaatg ccgataccat ttattccatg
     9721 agcaagaagg agccattatg gttcgcaaag ggtgtaattc attagtccgt gctgaaaaaa
     9781 tattaaccca cattgcatgg gttggtatgg caagttatat ggagttatta aataaattcc
     9841 agtaccccaa aagcagcctg cttaatttat tgaatgtaat ggtggagtgt ggtttcttaa
     9901 ttaaaaacaa aaacggctac tattcactgg gaataaaaaa ctacgaattg ggttgtcagg
     9961 cgttacaccg tcaaaatata tttgaggtga ctaagcggcc aatgcaggag ttgtcgctga
    10021 aaagtggact ggtttgtcat cttggggcga tggagagcat ttcggctatc tatctcgaca
    10081 agatcgaaag tccggactct gtaccgacga gcaaaagctg gattggcaaa aaacttgagt
    10141 tgcatattac cgcgttgggt aaggcattat tagcctggaa gacacgagaa gaactggatt
    10201 attttttaga agcactcacg ttaactccac atacgcgcaa tacatttacc gataaaaaat
    10261 tgtttctgga agaattacaa aaaacacgcc ttcggggatg ggccatagac aacgaagaat
    10321 caacctatgg ggcggtatgt ttaagtatgc cggtattcaa tatgtataac agagttaact
    10381 atgcaatctc actttctggc gatccggtag tttattcagg aaataagata gacagctatc
    10441 tggaattgct ccggaaatgc gctgagcaaa tatcatatgg gctgggatac agaaacgaaa
    10501 atgagcattt acgaaaagga aactgaggta atgaaaaaat tcagcggcat tattccaccg
    10561 gtatccagca cgtttcatcg tgacggaacc cttgataaaa aggcaatgcg cgaagttgcc
    10621 gacttcctga ttaataaagg ggtcgacggg ctgttttatc tgggtaccgg tggtgaattt
    10681 agccaaatga atacagccca gcgcatggcg ctcgccgaag aagctgtaac cattgtcgac
    10741 gggcgagtgc cggtattgat tggcgtcggt tccccttcca ctgacgaagc ggtcaaactg
    10801 gcgcagcatg cgcaagccta cggcgctgat ggtatcgtcg ccatcaaccc ctactactgg
    10861 aaagtcgcac cacgaaatct tgacgactat taccagcaga tcgcccgtag cgtcacccta
    10921 ccggtgatcc tgtacaactt tccggatctg acgggtcagg acttaacccc ggaaaccgtg
    10981 acgcgtttgg ctctgcaaaa cgagaatatc gttggcatca aagacaccat cgacagcgtt
    11041 ggtcacttgc gtacgatgat caacacagtt aagtcggtac gcccgtcgtt ttcggtattc
    11101 tgcggttacg atgatcattt gctgaatacg atgctgctgg gcggcgacgg tgcgataacc
    11161 gccagcgcta actttgctcc ggaactctcc gtcggcatct accgcgcctg gcgtgaaggc
    11221 gatctggcga ccgctgcgac gctgaataaa aaactactac aactgcccgc tatttacgcc
    11281 ctcgaaacac cgtttgtctc actgatcaaa tacagcatgc agtgtgtcgg gctgcctgta
    11341 gagacatatt gcttaccacc gattcttgaa gcatcagaag aagcaaaaga taaagtccac
    11401 gtgctgctta ccgcgcaggg cattttacca gtctgaggag aaactcatgt ctgttcgcaa
    11461 tatttttgct gacgagagcc acgatattta caccgtcaga acgcacgccg atggcccgga
    11521 cggcgaactc ccattaaccg cagagatgct tatcaaccgc ccgagcgggg atctgttcgg
    11581 tatgaccatg aatgccggaa tgggttggtc tccggacgag ctggatcggg acggtatttt
    11641 actgctcagt acactcggtg gcttacgcgg cgcagacggt aaacccgtgg cgctggcgct
    11701 gcaccagggg cattacgaac tggacatcca gatgaaagcg gcggccgagg ttattaaagc
    11761 caaccatgcc ctgccctatg ccgtgtacgt ctccgatcct tgtgacgggc gtactcaggg
    11821 tacaacgggg atgtttgatt cgctaccata ccgaaatgac gcatcgatgg taatgcgccg
    11881 ccttattcgc tctctgcccg acgcgaaagc agttattggt gtggcgagtt gcgataaggg
    11941 gcttccggcc accatgatgg cactcgccgc gcagcacaac atcgcaaccg tgctggtccc
    12001 cggcggcgcg acgctgcccg caaaggatgg agaagacaac ggcaaggtgc aaaccattgg
    12061 cgcacgcttc gccaatggcg aattatctct acaggacgca cgccgtgcgg gctgtaaagc
    12121 ctgtgcctct tccggcggcg gctgtcaatt tttgggcact gccgggacat ctcaggtggt
    12181 ggccgaagga ttggggctgg caatcccaca ttcagccctg gccccttccg gtgagcctgt
    12241 gtggcgggag atcgccagag cttccgcgcg agctgcgctg aacctgagtc aaaaaggcat
    12301 caccacccgg gaaattctca ccgataaagc gatagagaat gcgatgacgg tccatgccgc
    12361 gttcggtggt tcaacaaacc tgctgttaca catcccggca attgctcacc aggcaggttg
    12421 ccatatcccg accgttgatg actggatccg catcaacaag cgcgtgcccc gactggtgag
    12481 cgtactgcct aatggcccgg tttatcatcc aacggtcaat gcctttatgg caggtggtgt
    12541 gccggaagtc atgttgcatc tgcgcagcct cggattgttg catgaagacg ttatgacggt
    12601 taccggcagc acgctgaaag aaaacctcga ctggtgggag cactccgaac ggcgtcagcg
    12661 gttcaagcaa ctcctgctcg atcaggaaca aatcaacgct gacgaagtga tcatgtctcc
    12721 gcagcaagca aaagcgcgcg gattaacctc aactatcacc ttcccggtgg gcaatattgc
    12781 gccagaaggt tcggtgatca aatccaccgc cattgacccc tcgatgattg atgagcaagg
    12841 tatctattac cataaaggtg tggcgaaggt ttatctgtcc gagaaaagtg cgatttacga
    12901 tatcaaacat gacaagatca aggcgggcga tattctggtc attattggcg ttggaccttc
    12961 aggtacaggg atggaagaaa cctaccaggt taccagtgcc ctgaagcatc tgtcatacgg
    13021 taagcatgtt tcgttaatca ccgatgcacg tttctcgggc gtttctactg gcgcgtgcat
    13081 cggccatgtg gggccagaag cgctggccgg aggccccatc ggtaaattac gcaccgggga
    13141 tgtaattgaa attaaaattg attgtcgcga gcttcacggc gaagtcaatt tcctcggaac
    13201 ccgtagcgat gaacaattac cttcacagga ggaggcaact gcaatattaa atgccagacc
    13261 cagccatcag gatttacttc ccgatcctga attgccagat gatacccagc tatgggcaat
    13321 gcttcaggcc gtgagtggcg ggacatggac cggttgtatt tatgatgtaa acaaaattgg
    13381 cgcggctttg cgcgatttta tgaataaaaa ctgaaagcat aaataaaaac atccagacac
    13441 ctgaattatc cgactctgca tcattcgtta tttgctcagt cggataatga taggtcgcta
    13501 ccctgttatg agaggacgtt atgccactaa ttatcgttgt ggcagggatt gctttactcc
    13561 tgcttttaac cataaaaatt aagcttaata cgtttgtttc gttaattatt gtctcgattg
    13621 ctgtcgccat cgccagtggt atggatttga gtaaagtcgt cacctcagtc gaatccggac
    13681 ttggcggtac gctgggccat attggtttga tatttggctt cggtgttatg ctcggtcgct
    13741 tgctggccga tgcgggtggt gcgcagcgaa tagcgctgac catgctgaat tatttcggga
    13801 aaaataaact tgactgggca gtggtctgtt cggcgtttat tgtcggtatc gcactttttt
    13861 tcgaagtcgg tttaattctt ctggtaccta ttttattcgc tattgcccgt gaagcaaaaa
    13921 tatcaccgat gtttatgtgt gtgccgatgc tttctggttt gctggtcgca cacggttttt
    13981 tgccgccgca ccccggcccg actgttatcg ccagggaata tggcgctgat gtaggattgg
    14041 tactgatata cggtatcatt gtcggcattc caacctttat tctttgcggc ccggtattga
    14101 ataaattctg ccagcgcatt attccggacg cctttaaaaa agaggggaat atagcctctc
    14161 tcggcgcgac ccgccgattc agcgaaagcg aaatgccagg ctttgggatc agttttctca
    14221 cggcaatgct gccggtgatc ctgatggcgg ttgtcaccat catccagatg actcatgcca
    14281 aaagtgccgc ggattcaggc ttattttaca acgtgatact gttcttaggt aattcgacga
    14341 tcgccatgct gatttcactg ctgttcgcga tctataccat gggcctgggg cgcgggaaaa
    14401 caatccctga cctgatggat tcttgcggaa aagctatcgc gggtattgcc ggtctgctgc
    14461 tgattatcgg tggtggtggt gcattcaagc aagttctcat cgactctggc gttggtcagt
    14521 atatttccac cctggtttcg ggtatggata ttaacccgat cctgatggcc tggggcgtgg
    14581 ccgcattcct gcgtatttgt ctgggttcag ccaccgtcgc ggcgatctct accgccggtc
    14641 tggtcatccc attgttggca gtacatccca ataccaacct ggccttgatt acgctggcta
    14701 ccggtgccgg atcctgtatt tgttctcacg ttaacgatgc cagtttctgg atgattaaag
    14761 acttttttgg cctgaccacc aaagaaacgc tgttatcctg gacgctgatg tccacactct
    14821 tatccatcag tgggctgatt tttattctgc tcgccagctt agttttataa tcgggttctt
    14881 gccagtgagc caacctgttg ttcactgtcc cccataattt agtgagtgat ggcaaccgtc
    14941 tgcacggtga cgaatcgctg gcgatcaaaa aggggcgata ttgaaccgtc gattatgctt
    15001 gatgggtgta ctctcagtaa tactgtaaag ccggagcatg tctccggctt tacagtatgg
    15061 gggaacagcg ggcgatcgct caaatggtat gctggtttat ttcatgtaaa agaataaaat
    15121 atctgtataa aatcatgcca ctggcaatct gcatcaactc tccgggtaaa cagggttaag
    15181 atgagcattt ccccggttta atacaggtat acaaaagtgg atagagatca aacaaacagc
    15241 agcctgttca atgatgatcc cgtacttcat gcaacatggc tgtactatca ggaaggcaaa
    15301 agccagactg aagttgcagc tatcatgggg gtgtcccgcg tcacggttgt caaatacctg
    15361 caaacagccc gggaaaatgg actggttcat atcaacctgg atgtgaatgt ctttggctct
    15421 atcgacgctg ctttgcaaat tcgcgataaa ttcaatctcc agagagtgat catcgtgccg
    15481 gatggcgaac atgccggaaa acgtgatgat acaaagctga tgcggactcg cctttcacgt
    15541 gccggtggca tgtatctgaa tcaggtgatt gagaatggtg atgtgctggg ggttgcctgg
    15601 ggaagaacaa tccatcagat gagcaaaacc atgacgccga agtcatgtaa aaatgtcacc
    15661 gttatacaga tgctcggttc catgccatct cagccagacc tgacgatcat tgaatcctca
    15721 tcgcaaatcg cctacaagct ttcaggccgt atcgcctcgc tgcacgtgcc cgccgtggta
    15781 tcaagcgcca gactggccat ggagttacag gcagagccta ttattcgatc gaattttgac
    15841 gttctgactc gctgtacgaa agcctttttt gtcgtgggta acgcgcttga tgaaaatccc
    15901 ctgattcggg tgggcgtgct gaataagaag gaaatgcaga cgtatcggga tttaggcgca
    15961 gtaggtgtca tttgtggtcg cttctatgac aaggaaggga tgcccgtcgt ggctgatgtt
    16021 gaccagcgca ttctgggaat cagtctggct cagctaaggc agattgaacg gaagatcttc
    16081 cttgctggtg gggaaagagg ctatgacgca acgttgggtg ccctgctggg aggatatgtc
    16141 acggacctga ttgttgatga aggaacggca gagtttttgc tggcgtgtga attaccacac
    16201 taaaagaatg aacaactgcc ccatgtggat atggggcagg attttattga ttagcggcga
    16261 taataagaac aatacccaca atgtatgaaa taaacatggt cagcagcagt ttattggttc
    16321 cggctggcgc actggcaaat tctctccaga tgaagactcc ccagatcacg gcaatcattg
    16381 tggccccctg tcccagaccg taggaaatgg cataccccgc ctgaccagac gcaatcagac
    16441 tgaatgccag accgacacac caaatcatac cgcccagcca gccgcaaaca tgatcccgta
    16501 aactcccgga aaaatacatt ttcccgttga ccggttctcc tgaaataggt tttttcatga
    16561 ccagcgtgtt aaggacaaag ttcgacaaaa acagccccac ggcaaacaga acaagtgctg
    16621 agtaaggcgt catcagaccg ctggcaggct ggctgaaatt gtcagacatg gagtccgcca
    16681 ggaagcggaa aaaccatccc atgatgatac ccgccagaat ggcagtaatg agacctttat
    16741 tacggcgaga tttatccgcc tcctgcgtga cacgaccata ggctatcgcg gtaaaaataa
    16801 tggcgatcac aacgcaggcg acaccgagga acagaattag cggatcgccc tgcggattgc
    16861 cgatataggt ggtgatcaca cccagaggca gtgccagccc cacacccaca ggaaaagcta
    16921 ccgccatacc ggcaagatta atagaagcga ccagaagaat attggaaata ttgaagataa
    16981 tccctgccag tattgccgac atcagactac tgctgctcgc ctgctgaata tcaggtataa
    17041 aactgcgacc ggcttcgccc atagaacccc gcgtgaaggc aaataacaaa gaacacagca
    17101 ataatccaat tgaataatcc caatagaata gagggaactc ccatttttta ttgctgacaa
    17161 gtttggttgt attcgcccat gaaccccagc aaatcatggt aataaaacac attattatgg
    17221 cgacagcata cgattccacg ataaacataa ataactcctt gtggtagtta taatcaatcg
    17281 ttgtgtacag agggaatagc agccattctg cgaatggctg tcatatgtta ttaattaaaa
    17341 taaagctgta attacgcttt caaaccatac gttttgaatt tttcaaggac gatggtcatt
    17401 tcggcttcag acagcacggg aacatcgtga tgtaatccgc cacatttgat gtataaatca
    17461 gccagcgtct cggtttcacc cgccagccac aacgtctttt ccagcgtgac gtccatggcc
    17521 agcataccgt ggtgctgcat cagcaaggac ttactttctc tgatcccttt agatacaccg
    17581 tcggccagtt caggactgcc aaaggtataa tagggaatac agggaatatg atccgtcccc
    17641 gataccgcca ccatataatg tgagcagtgt ctgccagatg gtgacgggag acgggcagca
    17701 cgatacctcc cgtacatgcg tgtcgggtaa tgttttcata ctgaagattt tcctgatatg
    17761 cagagataaa aaatgggaca gtggcgtggt gaaaacacca ggccggagta gagggttatt
    17821 ccggtggttt actttttcgt ttcgggcgtc ggataaacag ccagataaac gtaaccacag
    17881 gagccgagg
//
LOCUS       DADSZD010000092        17783 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.092, whole genome shotgun sequence.
ACCESSION   DADSZD010000092 DADSZD010000000
VERSION     DADSZD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.092"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            102..176
                     /gene="tnaC"
                     /locus_tag="J1Z28_002803"
     CDS             102..176
                     /gene="tnaC"
                     /locus_tag="J1Z28_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBA7717622.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            397..1812
                     /gene="tnaA"
                     /locus_tag="J1Z28_002804"
     CDS             397..1812
                     /gene="tnaA"
                     /locus_tag="J1Z28_002804"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBA7717623.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1904..3151
                     /gene="tnaB"
                     /locus_tag="J1Z28_002805"
     CDS             1904..3151
                     /gene="tnaB"
                     /locus_tag="J1Z28_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBA7717624.1"
                     /translation="MTDQSEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDSTSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKKKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHAGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3283..4458
                     /gene="mdtL"
                     /locus_tag="J1Z28_002806"
     CDS             3283..4458
                     /gene="mdtL"
                     /locus_tag="J1Z28_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBA7717625.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIALLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4433..5392
                     /gene="yidZ"
                     /locus_tag="J1Z28_002807"
     CDS             4433..5392
                     /gene="yidZ"
                     /locus_tag="J1Z28_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBA7717626.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            5549..6298
                     /locus_tag="J1Z28_002808"
     CDS             5549..6298
                     /locus_tag="J1Z28_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717627.1"
                     /translation="MATHFARGILTEGHLISVRLPSLCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6320..6886
                     /gene="yieF"
                     /locus_tag="J1Z28_002809"
     CDS             6320..6886
                     /gene="yieF"
                     /locus_tag="J1Z28_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent chromate oxidoreductase YieF"
                     /protein_id="HBA7717628.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6940..8277)
                     /locus_tag="J1Z28_002810"
     CDS             complement(6940..8277)
                     /locus_tag="J1Z28_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7717629.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8444..9109
                     /gene="yieH"
                     /locus_tag="J1Z28_002811"
     CDS             8444..9109
                     /gene="yieH"
                     /locus_tag="J1Z28_002811"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBA7717630.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9176..9649
                     /locus_tag="J1Z28_002812"
     CDS             9176..9649
                     /locus_tag="J1Z28_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBA7717631.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLILLVIIQ
                     VLPLFLLLSITTGAIPAMLTGVMVACLPEKIGSQKRYRCLVGGIGGVVITEIYCAVIV
                     HIKDMASSALFENILSGENLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            complement(9731..10453)
                     /locus_tag="J1Z28_002813"
     CDS             complement(9731..10453)
                     /locus_tag="J1Z28_002813"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA7717632.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLITLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGSGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAVAELALG"
     gene            complement(10468..11637)
                     /locus_tag="J1Z28_002814"
     CDS             complement(10468..11637)
                     /locus_tag="J1Z28_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBA7717633.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     TDNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(11664..13280)
                     /locus_tag="J1Z28_002815"
     CDS             complement(11664..13280)
                     /locus_tag="J1Z28_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBA7717634.1"
                     /translation="MFRRNIITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSRAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAIVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVSANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKYNESGYKTTLFHTFKVNTSMLTSRPEIRFYTTYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13366..14760)
                     /locus_tag="J1Z28_002816"
     CDS             complement(13366..14760)
                     /locus_tag="J1Z28_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBA7717635.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVVLWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKTCHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRILLNTLWDRYQKPLFIVENGLGAKDSVEVDGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(14779..16653)
                     /gene="bglF"
                     /locus_tag="J1Z28_002817"
     CDS             complement(14779..16653)
                     /gene="bglF"
                     /locus_tag="J1Z28_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBA7717636.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKVQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMVATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFSGNPFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKRQPAQVAQQEKTPEVITPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVAGQIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(16787..17623)
                     /gene="bglG"
                     /locus_tag="J1Z28_002818"
     CDS             complement(16787..17623)
                     /gene="bglG"
                     /locus_tag="J1Z28_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBA7717637.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4540 a         4461 c         4124 g         4658 t
ORIGIN      
        1 cccgaacgat tgtgattcga ttcacattta aacaatttca gaatagacaa attccttgag
       61 tattatactg tagcctcgtg tctttcgagg ataagtgcat tatgaatatc ttacatatat
      121 gtgtgacctc aaaatggttt aatattgaca acaagattgt cgatcaccgc ccttgatttg
      181 cccttctgta gccatcacca gagccaaacc gattagattc aatgtgatct atttgtttgc
      241 tatatcttaa ttttgccttt tacaaaggcc atctctcgtt tatttacttg ttttagtaaa
      301 tgatggtgct tgcatatata tctggcgaat taatggatac tgcaaatgta atattcacag
      361 ggatcactgt aattaaaata aatgaaggat tatgtaatgg aaaactttaa acatctccct
      421 gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta ccactcgcgc ttatcgtgaa
      481 gaggcaatta ttaaatccgg tatgaacccg ttcctgctgg atagcgaaga tgtgtttatc
      541 gatttactga ccgacagcgg caccggggcg gtgacgcaga gtatgcaggc cgcgatgatg
      601 cgcggcgacg aagcttacag cggcagccgc agctactacg cgttagccga gtcagtgaaa
      661 aatatctttg gttatcaata cactattccg actcaccagg gccgtggtgc agagcaaatc
      721 tatattccgg tactgattaa aaaacgcgag caggaaaaag gcctggatcg cagcaaaatg
      781 gtggcgttct ctaactattt ctttgatacc acgcagggcc atagccagat taacggctgt
      841 accgtgcgta acgtctatat caaagaagcc ttcgatactg gcgtgcgtta cgactttaaa
      901 ggcaactttg accttgaggg attagaacgc ggtattgaag aagttggccc gaataacgtg
      961 ccgtatatcg ttgcaaccat caccagtaac tccgcaggtg gtcagccggt ttcactggca
     1021 aacttaaaag cgatgtacag catcgcgaag aaatacgata ttccggtggt catggactcc
     1081 gcacgctttg ccgaaaacgc ctatttcatc aagcagcgtg aagcagaata caaagactgg
     1141 accatcgagc agatcacccg cgaaacctac aaatatgccg atatgctggc gatgtccgcc
     1201 aagaaagatg cgatggtgcc gatgggcggg ctgctgtgca tgaaagacga cagcttcttt
     1261 gatgtgtata ccgaatgcag aaccctttgc gtggtgcagg aaggcttccc gacatatggc
     1321 ggcctggaag gcggcgctat ggagcgtctg gcggtaggtc tgtatgacgg catgaatctg
     1381 gactggctgg cttatcgtat cgcgcaggtg cagtatctgg tcgatggtct ggaagagatt
     1441 ggcgttgtct gtcagcaggc gggcggtcac gcggcattcg ttgatgcagg taaactgttg
     1501 ccgcatatcc cggcagatca gttcccggca caggcgctgg cctgcgagct gtataaagtc
     1561 gccggtatcc gtgcggtaga aattggctct ttcctgttag gccgcgatcc gaaaaccggt
     1621 aaacaactgc catgcccggc tgaactgctg cgtttaacca ttccgcgcgc aacatatact
     1681 caaacacata tggacttcat tattgaagcc tttaaacatg tgaaagagaa cgcggcgaat
     1741 attaaagggt taacctttac ctacgaaccg aaagtattgc gtcacttcac cgcaaaactg
     1801 aaagaagttt aattaaaaca ctcagagtgg ctataaggat gttagccact ctattaccct
     1861 acatcctcaa taacaaaaat agccttcctc taaaggtggc atcatgactg atcaatcaga
     1921 aaaaaaacac tctgcatttt ggggtgttat ggttatagcc ggtacagtaa ttggcggtgg
     1981 gatgtttgct ttacctgttg accttgccgg agcctggttt ttttggggcg cttttattct
     2041 tattatcgcc tggttttcaa tgttacattc agggctatta ttattagaag caaacttaaa
     2101 ttatcccgtc ggatccagtt ttaacaccat caccaaagat ttaatcggta acacctggaa
     2161 cattatcagc ggtattaccg ttgccttcgt tctctatatt ctcacttatg cctatatctc
     2221 tgctaatggt gcgatcatta gtgaaacgat atcaatgaat ttgggctatc acgctaatcc
     2281 acgtattgtc gggatctgca ctgccatttt cgttgccagc gtattgtgga taagttcgtt
     2341 agccgccagt cgtattacct cattgttcct tgggctgaag attatctcct ttgtaattgt
     2401 ttttggttct ttcttcttcc aggtcgatta ctccattctg cgcgattcca ccagcaccac
     2461 tgcgggaacg tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt
     2521 tggtttccac ggcaatattc ccagcctgat tatttgctat ggcaaaaaga aagacaaact
     2581 aatcaaaagc gtggttttcg gttcgctgct ggcgctggtg atttatctct tctggctcta
     2641 ttgcacgatg gggaatatcc cgcgagaaag ctttaaggcg ataatctcct caggcggcaa
     2701 cgttgattcg ctggtgaaat cgttcctcgg caccaaacag cacggcatta tcgagttttg
     2761 cctgctggtg ttctccaact tagctgttgc cagttcgttc tttggtgtca cgctggggtt
     2821 gttcgattat ctggcggatc tgtttaagat tgataactcc cacgccgggc gtttcaaaac
     2881 cgtgctgtta accttcctgc cacctgcttt gttgtatctg atcttcccga acggcttcat
     2941 ttacgggatc ggcggtgccg gactgtgtgc caccatctgg gcggtaataa ttcctgctgt
     3001 actggcaatc aaagcccgca agaagtttcc caatcagatg ttcacggtct ggggcggcaa
     3061 tcttattccg gcgattgtca ttctctttgg cataaccgta attttatgct ggttcggtaa
     3121 cgtctttaac gtgttaccta aatttggcta aatccttcag gaagccagcc attcgctggc
     3181 ttcttgcctg tcaggaaatc acttatgtcc aaatggcaac tcgcctgatt ctccttcacc
     3241 acgtatgctt tgcgtcacct tactatcagg acgctttagc ccatgtcccg ctttttgatt
     3301 tgtagttttg ccctggtttt actttatccc gcagggattg atatgtacct cgttggttta
     3361 ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt tgcatattgc gttctccgtt
     3421 tatctggcgg ggatggcggc tgcgatgtta tttgccggta aagtggccga tcgttcaggg
     3481 agaaagcctg tcgccatacc cggcgcggcg ctatttatta tcgcctcggt gttctgttca
     3541 ctggctgaaa ccagcgcgtt atttcttgca gggcgatttc tacagggact gggcgcgggc
     3601 tgttgttacg tagtggcgtt cgccattttg cgcgacacgc tggatgatcg acgtcgggct
     3661 aaagtgctgt cgttactcaa cggcattacc tgcatcattc cggtgttagc gccggtgctc
     3721 ggacacctga ttatgcttaa attcccttgg cagagtctgt tctggacgat ggcaacgatg
     3781 ggcatcgcgt tactgatgtt gtctttgttt attttaaaag aaacgcgccc tgcggccccc
     3841 acaacttcgg acaaaccacg agaaaatagc gagtcactgc ttaatcggtt tttcctcagc
     3901 cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca ccttcgtcaa cacgtcgcca
     3961 gtattgctga tggaaatcat gggttttgag cgcggagaat acgccaccat tatggcgttg
     4021 accgctggcg tcagcatgac cgtttcattc tccacgccgt ttgcgctggg aatttttaag
     4081 ccacgtacgt tgatgatcac ctcacaggtg ttattcctgg cggcggggat cacccttgcc
     4141 gtttcacctt cccatgcggt ttcgctgttt ggtatcacgc tgatttgcgc cggtttctcg
     4201 gtaggttttg gtgtggcgat gagtcaggcg ttagggccgt tttcattacg cgcgggcgta
     4261 gccagctcga ccttaggtat tgcgcaagtt tgcggttcgt cactgtggat ttggctggcg
     4321 gcggtggttg gtatcggcgc atggaatatg ctgatcggga ttctgattgc ctgtagcata
     4381 gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc cggttgccgc tcatgaagaa
     4441 atccatcacc acgcttgatc tcaatctgct gctctgtctg caactgctga tgcaggagcg
     4501 cagcgtgacc aaagcggcga agcggatgaa cgtgacccct tcggcggtga gtaagtcgct
     4561 ggcaaagtta agagcgtggt ttgacgaccc gctctttgtg aactcaccgc tggggctgtc
     4621 gcccacaccg ctaatggtta gcatggagca aaatctggcg gagtggatgc aaatgagcaa
     4681 cctgctgctg gataaaccgc accaccagac accgcgcggc ctgaagtttg agctggcagc
     4741 agaatcaccg ctaatgatga tcatgcttaa tgcgctgtcg aaacggatct accaacgtta
     4801 cccgcaggcg accatcaaat tacgtaactg ggattacgat tccttagatg ccattactcg
     4861 tggtgaagtg gatattggtt tttccggtcg cgaaagtcat ccccgttcgc gggagctgtt
     4921 aagctcgcta ccgttagcca ttgattatga agtgctgttt agtgatgtgc cctgcgtctg
     4981 gttacgcaaa gatcatccgg cactgcatga agcatggaat ctggacacct ttttacgtta
     5041 tccgcatatc agcatttgct gggaacagag cgatacctgg gcgctggaca atgtgttaca
     5101 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
     5161 tatggcagca cagcccgaca atctgctact ggcgaccgcg ccgcgctact gccagtacta
     5221 caatcaactc caccaactgc cgttggttgc tcttcctctc ccgtttgatg aaagccagca
     5281 aaaaaagctg gaagtccctt ttacccttct gtggcataaa cggaacagcc gtaatccgaa
     5341 gatcgtctgg ttacgggaaa ccattaaaaa tctttacgcg tcgatggcat aaccgaatcg
     5401 tatgaaacgc gacccaattt cacaatgaaa tgtaaaaaag ttgtaataaa cttatctgaa
     5461 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
     5521 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
     5581 ggaaggacat ctgatttctg ttcgtctccc ctccctgtgt catcaagaag cccgaaacat
     5641 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
     5701 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gaaaaccggt
     5761 tttcagcgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggtcggcgt
     5821 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacacgcgg
     5881 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
     5941 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt
     6001 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcaac tactgcccat
     6061 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     6121 cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa aagctcagtg tctgggagtt
     6181 agatggcaaa cacggctgga aaagcctgcc cgatattcac agccgcgcca acaacccgac
     6241 cagccggatg atgcgttttg cccagctctc taccgtgaag gctttttcgc caaattgata
     6301 gacaaccaca ggagtcatca tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     6361 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     6421 catggaaatc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     6481 gcaggaagac ggttttccag caacagttga agctctggcg gaacagatcc gtcaggctga
     6541 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     6601 catcgactgg ctttcccgcc tgccggatca accgctggct ggtaaaccgg tattgattca
     6661 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     6721 ggttttcctc gatgcaatgg tgatgaacaa gccagaattt atgggcggcg tgattcagaa
     6781 caaagttgat ccgcaaaccg gggaagtgat tgatcagggt acgctggacc acctgaccgg
     6841 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     6901 agcaatcatg catggcgggt ttttaacgcg ctatcgattt tagtgagcgt cgataaagac
     6961 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     7021 tccggtacca atcttcatca cgcagtagga gataaagccc agcgcgatac cttcagtaat
     7081 cgagaagctg aacggcatca tcacagcggt aataaacgcc ggaacagatt cagtaagatc
     7141 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaaa tcagcgcacc
     7201 agctgcagcg tagcctggca ccatccccgc cagcggcgac aggaagataa ccagcaggaa
     7261 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaacggaaa cgccggaaga
     7321 agattcaata tacgccgtaa cagaagaagt accgataaac gaaccggtca ccgaagagat
     7381 actgtcgaca tacagcgcct gcttcatgcg cgggaattta cctttctcat cagccagacc
     7441 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccagcatgaa
     7501 agagaaaatc acccctgcca gcccaaggtt aaacgacccg gctaaatcta catgacccac
     7561 gacactcatt acgctcggcg gcgcagaaac gatgccatta tagtggacat cacccagcat
     7621 ccagcccagc agcgttgtca ccacgatgga aaccagcacc gccgcgtgaa tattgcgcga
     7681 ggccagaatg gcgatgatga agaagccgag gatccccagc agcacgctgt gagaagtcag
     7741 attaccgatg ctcaccagcg tttccgggtt agcgacaatc acacctgcgt ttttcagccc
     7801 catcatgcca ataaacagac cgataccact ggtgatgccc acacgtagac ttaccgggat
     7861 gttggctatc atccagtagc gaacgcggaa aatcgtcagt aacagcagac caatcgcgcc
     7921 ccagaagatt gcgcccatcc cgacttgcca cggcaagccc atcgcctgta caacgacaaa
     7981 agcgaagaac gcattcaggc ccattgccgg tgccagtgca actggcaggt tagcaaacag
     8041 ccccatcata atactgccga atgcagcaat cagacaggtc gtgacgaaga cggcgctggt
     8101 atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag
     8161 gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt
     8221 aaacacgcgt tccagcatcc cctggccaga agcctgggtt gtgtgttgtt gactcattat
     8281 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatga gacaatatgg
     8341 acggctgaat ataacttttt ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg
     8401 aattggcatg acgaaaacgt taaactaacg ataaaggaaa gacatgtccc agatagaagc
     8461 ggtatttttc gactgcgacg gtacgctggt cgatagcgag gtcatttgct ctcgcgccta
     8521 tgtcacgatg ttcagggaat ttggcattca cgttgacctg gaagagattt ttacccgttt
     8581 taaaggcgta aaactctacg aaatcatcga catcatcagt aaagaacagg gcgtgacgat
     8641 ggtccaggcc gatgccgagc acgtttaccg cgcagaagta gcacgtctgt ttgataccga
     8701 acttgaggac atcgcaggag cgaacgaatt gctggcaagc attaacgtgc cgatgtgcgt
     8761 cgtctctaac ggtccggtca gcaaaatgca gcattcgctt ggtaagctca acatgctgca
     8821 ctacttcccg gaaaaactgt tcagcggcta tgatatccag cgttggaagc ccgatccggc
     8881 attaatgttc catgcggcaa aagcgatgaa tgtgaacgtt gaaaactgca ttctggtcga
     8941 tgactccagc gccggtgcgc aatcagggat agatgccgga atggaagtgt tttacttctg
     9001 cgctgacccg cacaataaac cgatcgttca cccgaaagtc accaccttta cccatctttc
     9061 gcaattaccg gaactgtgga aggcgcgtgg ttgggatatt acggcatagt tattcacact
     9121 cccttcactt actccactta aattggcacg caaaggtaag taaagggagt ttgatatgtc
     9181 tgtttcacgt cgggtaattc atcacggact gtattttgcg gttttagggc cgttaattgg
     9241 tgttctgttt cttgtcctct acatattctt cgcaaaagaa ccgctgattc ttttggtaat
     9301 aatacaggta cttccattgt ttctcttatt gtcgataact acgggagcta ttcctgcaat
     9361 gttaactggc gtaatggtcg cctgtctgcc ggaaaagatc gggtcacaga aacgttatcg
     9421 ttgtctggtt ggcggcatag gtggagtcgt tatcaccgag atctattgcg ctgttatcgt
     9481 acatattaag gacatggctt cctcagcgtt atttgaaaac attctttctg gtgaaaatct
     9541 cgttgtccgc atcatcccag cattgctggc aggcgtggtg atgagcagaa tcattacacg
     9601 tctacccgga ttggatattt catgtcctga aacagactct ttaagctaag cggaatgttt
     9661 tatctatttg tcattaaatg ttggagaaca ggccggagcg taattcacac atccggcctt
     9721 atttttaagc ttaaccgagc gctaattctg cgacagcggc tttatcagcg attaccatca
     9781 gcgaaggatg cagctgcaac acggaagctg gaacatcttc ggtaaccgga ccttgcagaa
     9841 catttttcag tgcctgcgct ttacctgaac cactgacaat tatcagcaag tttttcgccg
     9901 ccatgatgct tttcggcccc atcgtcacgt agctgtcagg cactaatgaa aagtcgccac
     9961 ccaactcgcc atgcgccaca atatcaacca tctctccctg gatcgggaac tccacggttt
    10021 gctcatggaa atgggtcgta tttggcaaat tgccacaaaa atgaccatct gcacctaatc
    10081 ccagcaccac caaatccagg ccgccttcac gcgccagttt ctgatcatgc tcgcggtagt
    10141 tatcaatagt gagcttctgg atattctctt ctttgatccc cgcaggggtg aaaaacagat
    10201 tacgcagatt ggtaatcgtt acgccttctc cctctttgcc gcgaaatgga atttcatcga
    10261 aattatagaa atagcagtta tcgtaccagg gcttaccttt aaccagagtg atgagatatt
    10321 cgtacatgcc tttgggcgtg ctcccggcgg taattgccag gttaacacga cgcgtcttcg
    10381 acatataacc aagcagatgg tgtgccgcga cacggctcat ttcctggtaa tcttcggtaa
    10441 tgattaattt catcttaatg ccttatatta tttgaataat ttctgtacga aggctgcata
    10501 ggcaggccgc cagacatcca tttcatggtt cagacccgga tattcctggt aatcaaactt
    10561 aatttttttc tgctcaagct cagttttcag cccggcgata tccttgccgg ttacaacatc
    10621 tttatcccca acaaccacag taaaattacg tagttgctgg ttaatagctg ccggctcgtt
    10681 cagccgggca gcgacccctt catctggtac ggtcgtcgtg gtaacaccac tgaatgtggc
    10741 cagccagcca aagctttcca gatgattcat tcccgatacc agtgcctgat acccaccttg
    10801 tgaaagccct gccagtgcgc gaccatcggc atctttacgg acattaaaac gcttgctaat
    10861 cagcgggata atatcgttca tcagttcgcg atctgccgct ttagcattta acggataaaa
    10921 gactttacgt ctctcctgag gaacgaaatc ttcgggaata atgcccttcg catcggtttc
    10981 ggtatccggg atcaccacca gcatcggttt aattttccct tcagccagca ggttatccat
    11041 gatttgcggg atacgcccct gatcaatagc ggaacgtccg gtatcaccaa agccgtgata
    11101 gaaatagagc actggcaaag gctcgcctat gccggtgtat cctggcgggg tccagacata
    11161 catctgacgt tcggattgca atgcgttgga gtggtaagtt atggcgatca aatcaccatg
    11221 cacaacagaa cgtgtatcca gataactgcc cggtaccaga atcatactgg agttaacctg
    11281 acgctgaggc ttagtcattg ccgtgcctgt atcaatgctg cgtacaccat caacattgaa
    11341 aaaatactcg tacagattgc ctttcaggac aggcgtgcgc cacgaccaga ctcccgcttc
    11401 atctttggtc atcgggtgaa tattgtccgg aaccggaaca cccaccacta cggagacatt
    11461 ttttgcccca ggggcaaagt agcgaaaggt cacgctgtta tcggtattga cctgagtcac
    11521 atactgtttc accggaatag tgggtgccgg gctggctggc atatccgctg ctatggccca
    11581 ttgagcagaa ataccgctgg caatagccag cgttaaagca gcaattttta tattcatcgt
    11641 aatattcctt ttattaagtt ccattaccac cagatttcag cctgggcacc gacagcaaac
    11701 tggtcgtctt tattgtcttc aaaggtgaag ccatccagtt cgttttccag agctttgata
    11761 tatgtagtgt agaaacgaat ttccgggcgc gaggtcaaca tactggtatt gactttaaag
    11821 gtatggaaga gcgtggtttt ataaccagac tcattatatt tattgctatt cgcatctttg
    11881 ttttgctggg tgaaatagcc cagttcaaca cctgtctggt tatattggtc ccaaatatag
    11941 gccgggcgaa caaccgcacg aatagattcg aaatcagaat gtgcgcctgt ttcgtagcta
    12001 taaatatcgt taccgaagga gtaaacaatc gcgttagcca caatgaaatg atcgccgata
    12061 taagcttcgc cctgcgaagt cagacgaact gcagttccgc ctgtgtgatc accataataa
    12121 cgaccattaa aggtagtaaa tggacttgca ccagcataac gaccaaagtt actggcgata
    12181 gagttattcg caaccaggaa ggagaattcg ttgaagccac ccttatcaaa tttctgcgtt
    12241 aaagatgtac caaacatcca ggtatctttc cagtcataat acccgttatt atcctgatta
    12301 tctttttccg atgcgctttc atttgctgag acataacgac cgctcaccat taaggtggct
    12361 ttatcccata acgggatatc tttatagcgt aaatcaatgg tattagtatt aatctgctgt
    12421 ttgttttgca ggctgcgatc gtaatcatcg acatcttcac gaaccagcgc gatatcaatt
    12481 ttacccggac cgactttcca gttttccaga cctacacctg cggcagcatc ggtacgctgc
    12541 gttttccagt caagcatctg gatttcaatt ttcggcgcac cgtgtttacc cacccagaaa
    12601 tcagcctctg gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc agagaactgc
    12661 ataaagttct caccaccggc gttatcgcca aaccagccag tagagtactg ctgaccaacg
    12721 ttaccatcca tcatcacgat ggcatcaacc cgtttgccgt tttcgttgta gacacgttgt
    12781 tttaactgca aatcaaacca gccggagtat tcgttaccaa agcggcccag agaaccaatt
    12841 gcccatgatt taggtgaacc atgagaggct gttccccaac cagaacgata gtagccgttg
    12901 taactaaagc cgatttcatc tttcacaaat ttactgaaat ctttcagagt catggaagaa
    12961 taaatcgagc ctgtctcact ggcatttttc tcttcatttt taaccagtac agcatcaggc
    13021 tttgcggcac gactggtcgg gaatggattg gcggcatacc cttgatcatt cgtacttacg
    13081 ctacgattca ccgtcgctgg cgtatacttt ttcttctctt catctttata ttttttcagt
    13141 tcgctttgcg tttctcttaa cgccttttct aatagctcaa ggcgttgttc cactgttaat
    13201 gattcagcca atgattgtgc ggaaaaggct aacggtgcca ttaataagat ggcagaggta
    13261 ataatatttc gtctaaacat aatatccctt tatggtgcaa agaaagaatt aacgcatcgc
    13321 atccagactg ttctgaatgc gacgataatt aaggtgcttt attggttatt tttttaatga
    13381 tagcccccga gttttgatca cttctgcata ccatccgaag cttttcttgc gcgttcttgt
    13441 gaggcttcct tcaccattat catcacgatc cacataaata aagccgtagc gcttagacat
    13501 ttgtgaatga gatgcactga ctaaatcaat tggcccccaa ctggtgtacc ccataatatc
    13561 cacaccatcg gcaatcgctt catttacctg taccaggtga tcgtttaaat aggcaattcg
    13621 ataatcgtcc tgtatcgaac catccacttc aacgctgtct tttgcgccta atccgttctc
    13681 gacaataaat aacggttttt gataacgatc ccaaagcgta tttaacagaa tccgtaatcc
    13741 aaccggatca atttgccacc cccactctga acttttcaga tgcggatttg ggatcatatt
    13801 cagtatgttg ccctgcgcat ttttattaat gctttcgtcg tgggaaacac aaccggtcat
    13861 gtaataacta aaagagatga aatctacggt atgttttaaa tcttctgcgt cactttcagt
    13921 catctcaatg gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg gatactgacc
    13981 acgcgcctga acatcaccaa agaacatcca gcgccggttc tcttccatgg cctgcaacat
    14041 atcctgtggc tggcaggtga ggggataaac cagcccaccg agaagcatat tgccaatttt
    14101 cgcttcgggg atcaggctat gacaggtttt aactgcccgc gcactggcaa ccagttgatg
    14161 atggatcgcc tgataaactt ccgcctcgcc actctcttct gccagcccca cgcccgtgaa
    14221 tggcgcgtgt aatgacatgt tgatttcatt aaatgtcagc cataacacaa ctttatgttg
    14281 gtagcgggta aagaccgtgc gtgcgtaatg ctcgaagtga tcgatgaccg cccgattagc
    14341 ccaaccgccg tagtttttca ccagcccata tggcatttcg taatgggata acgttaccag
    14401 cggcttgatc cccgcctgcg ccatttcatc aaacagccgc tcgtaaaacg ctaaccccgc
    14461 ttcattcggt tcggcttcgt cgccctgagg aaaaattcgc gcccaggcaa tggaaatacg
    14521 cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac ggtgataaaa
    14581 atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca tttttcccat
    14641 tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcct gccaggcgcc
    14701 ttccacctga ttggcagctg ttgcaccacc ccaaagaaat gtttctggaa atgctttcat
    14761 aattaactcc ttttatcgtt agcgaatgat ggataacagc ggctcaccag cgtttatctg
    14821 cgccgtgccg tggggtaata cgtccgtaaa atcatcgcta ttactgatta ataccggcgt
    14881 cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca gccgatcgcc
    14941 tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt ccagttttac
    15001 ggtgtcgata ccgacatgaa tcaggatctc cacgccatca tctgactcaa tgccaatggc
    15061 gtgtaatgtg gcgaacaacg aagcaatttg ccccgccacc ggagaacgca cttcaccaac
    15121 agagggcaga atggcaatac ctttacccaa caggccgctg gcaaacgtgg tatcagcgac
    15181 gtgaatgagc ggcacaatct ctcccgtcat cggtgaacag ataccgccct gctcaggtgt
    15241 aataacctct ggtgttttct cttgctgggc aacttgcgct ggctgacgtt tagcggtgat
    15301 gaaatgaaac atcaccgtac cgacaaatgc gcaaccgatg gcaatgacac cgccaataac
    15361 gctggcccat acggtgaaat caatccccgt tgacgggatg gtttgcatga aggtgaaaat
    15421 acctggcaaa ccaaaggagt aaactttcgt ttgcgcgtag ccaatgatgg tggcccccaa
    15481 tgccccactt atgcaggcga taacgaaggg gtacttacgc ggcagattga cgccatatac
    15541 cgctggttcg gtgataccaa acaaacctgt caacgccgct gatcccgcca ccactttttt
    15601 ctgcgcatcg cgttcgcaga ggaagacgcc gagcgccgcc ccgacttgcg ccataatggc
    15661 gggcattaac agcgggatca tggtgtcgta gcccagcacg gtgaagttat tgataattaa
    15721 aggtatcaaa ccccaatgca gaccgaacat cacaaagatt tgccagaaac cgcccattac
    15781 cgcgcctgca aatgcaggaa ccgcctgata aagccagaga taaccggcag caatcagttc
    15841 acttatccag gttgatagcg gccccaccag cagaaaggtg acgggtgtga taaccatcag
    15901 acatagcaat ggtgtgaaga aatttttgat tgccgacggt aaccacgcat taagtcggcg
    15961 ttccagaatg ctgcacaacc aggcagaaaa aataatggga ataaccgatg acgagtaatt
    16021 caacaatgtg accggaatac ccaggaaatc caggcccagc gcatccactt tttgcccgtt
    16081 ctcgaaggca gtcagaatta atggatgcac taacgctccg ccaatcacca tggcagtaaa
    16141 tggattgccg ctgaagcgtt tccctgcggt gtagcccagg attatcggga agaaccaaaa
    16201 caaggcatca ctggcgctaa ataaaattaa ataagtacca ctttgttcgg tcgtccactg
    16261 aaaagtgagt gccagagcca acataccttt caggatcccg gttgccacca tcaaaccgat
    16321 cagaggcgta aaaatacctg aaataacata aacaaagcgg tttaacagat tacctttatc
    16381 atcattttcc ggtgcctgtt gcactttttc gccaaggcct gccacgctgt taaccgccag
    16441 gaagacatcg gccacatggt tacctatgac aacctgaaac tggccaccgc tttccaccac
    16501 cataataata ccgggggtct ttttcagtac ctcagcttgc gctttgcttt catcctttaa
    16561 cttaaaacgt aatcgcgttg cacaatgcat cagactcaca atgttatctg cgcccccgac
    16621 tcctgcgact attgttctgg ctaactccgt cataacttgc cctctaccgc tttgcggcaa
    16681 aactccaaaa aaaaacctga aaaaaacggc ctgacgtgaa tcaagcaatt tttttcaggt
    16741 tttgcccgct tagtgcggta acaatccttt attcagtaat aatatttcag tgttctttgc
    16801 gcacgcgctc tatatttatg gctaaaaaca taatctctgc aggtgagatt ttacgttgat
    16861 actgcaaacc aataaaaatg gcgatccgct ccgcacattg ccatgcttgc gggtaatttt
    16921 gctttactgc ttgttgtaat gattcatcac tatcgttaat cgatgcatgt tcaagaatac
    16981 gccaggataa aaatttcaga tgcgtaacca gtcgctgata actcaagctt tcttcctggt
    17041 aattaaggct gaactgaaat tttattaatt gcagcatttc tcgcattaat tgcgtgacac
    17101 ctgcaacatc ctccatattt ccgctcattt gggcactgac cagatgcatg gcaataaagc
    17161 ccacttcatc tttcggtaac tgcacgccca accgtttatc aataatggtt aacgcttctt
    17221 cccctagctg gaactctttc gggtaaagcc gctggatatc ccacagcagc gggttgggca
    17281 gtagcacgtt ttgctgaaag cgtttaatcg caaactggca atggtcagtt agcgagatat
    17341 aaatactgtc ctgcaacttt cccaggcgct cctgcgccag agagataata cgatcacagg
    17401 ttgccatcac ctcaagaggc atatgactta agagttcgct taatcgcccg ttcagttcat
    17461 gactgctcaa ggcatactct ttttctattc cacttgagtt aattctttcg cctgggcgtt
    17521 tttgaaagcc aattccgcgc cccatgacga ctttttcccg ctgttgatca tcaataacca
    17581 ccacaacatt attgttgaga attttggtga tttgcatgtt catagcaagg acctttttta
    17641 taaacaaaaa aacccgactt caccagtatt ctctggttat gtcaggtttt gcctgcgaat
    17701 gcagtaacaa tccagtcatt aattaatggt ttttataacg aacatccagg ttcgaaaatt
    17761 aatttagttg cgtgcagaga aaa
//
LOCUS       DADSZD010000093        17481 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.093, whole genome shotgun sequence.
ACCESSION   DADSZD010000093 DADSZD010000000
VERSION     DADSZD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.093"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(71..1474)
                     /locus_tag="J1Z28_002819"
     CDS             complement(71..1474)
                     /locus_tag="J1Z28_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7717638.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDATP
                     TGHKASILKSFYAIFSNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            complement(1517..2902)
                     /locus_tag="J1Z28_002820"
     CDS             complement(1517..2902)
                     /locus_tag="J1Z28_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7717639.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGEMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            complement(2948..4984)
                     /gene="yihQ"
                     /locus_tag="J1Z28_002821"
     CDS             complement(2948..4984)
                     /gene="yihQ"
                     /locus_tag="J1Z28_002821"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBA7717640.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            complement(5090..5965)
                     /locus_tag="J1Z28_002822"
     CDS             complement(5090..5965)
                     /locus_tag="J1Z28_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBA7717641.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFIPRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            complement(6026..6928)
                     /locus_tag="J1Z28_002823"
     CDS             complement(6026..6928)
                     /locus_tag="J1Z28_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBA7717642.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRDWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            complement(6995..8236)
                     /gene="yihS"
                     /locus_tag="J1Z28_002824"
     CDS             complement(6995..8236)
                     /gene="yihS"
                     /locus_tag="J1Z28_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBA7717643.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            complement(8252..9130)
                     /gene="yihT"
                     /locus_tag="J1Z28_002825"
     CDS             complement(8252..9130)
                     /gene="yihT"
                     /locus_tag="J1Z28_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HBA7717644.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            complement(9154..10050)
                     /gene="yihU"
                     /locus_tag="J1Z28_002826"
     CDS             complement(9154..10050)
                     /gene="yihU"
                     /locus_tag="J1Z28_002826"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBA7717645.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            10218..11114
                     /gene="yihV"
                     /locus_tag="J1Z28_002827"
     CDS             10218..11114
                     /gene="yihV"
                     /locus_tag="J1Z28_002827"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBA7717646.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            11148..11933
                     /locus_tag="J1Z28_002828"
     CDS             11148..11933
                     /locus_tag="J1Z28_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7717647.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            12032..12631
                     /gene="yihX"
                     /locus_tag="J1Z28_002829"
     CDS             12032..12631
                     /gene="yihX"
                     /locus_tag="J1Z28_002829"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBA7717648.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            12625..13497
                     /locus_tag="J1Z28_002830"
     CDS             12625..13497
                     /locus_tag="J1Z28_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBA7717649.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            13494..13931
                     /gene="dtd"
                     /locus_tag="J1Z28_002831"
     CDS             13494..13931
                     /gene="dtd"
                     /locus_tag="J1Z28_002831"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBA7717650.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            13976..14917
                     /gene="fabY"
                     /locus_tag="J1Z28_002832"
                     /gene_synonym="yiiD"
     CDS             13976..14917
                     /gene="fabY"
                     /locus_tag="J1Z28_002832"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBA7717651.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(14981..15889)
                     /locus_tag="J1Z28_002833"
     CDS             complement(14981..15889)
                     /locus_tag="J1Z28_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7717652.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            16118..16429
                     /locus_tag="J1Z28_002834"
     CDS             16118..16429
                     /locus_tag="J1Z28_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7717653.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKCAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            16430..16720
                     /locus_tag="J1Z28_002835"
     CDS             16430..16720
                     /locus_tag="J1Z28_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7717654.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            17079..17357
                     /locus_tag="J1Z28_002836"
     CDS             17079..17357
                     /locus_tag="J1Z28_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717655.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPTLTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
BASE COUNT         4395 a         4348 c         4509 g         4229 t
ORIGIN      
        1 cataataagt ccatttttta aacgtaggga ggccgccacc catcaggatg gaaatagcgg
       61 ctattgacat ttataacgtt acggaagccg ttttttcttc tgtctcaatt tctttatttt
      121 tgcgttggtt aatttcctca ataatcagtg caaaccgttt ctcattgagc gtgtaaaaga
      181 accccatcgt caatgcagca ataattgcca gcgcacaagg ccagataaaa atgagctgac
      241 gcaaaccctg taatgttgca tcgctttgcg cgatgttggg aacataaccg atttgcgtca
      301 gcataatgcc tggcaagaag ccagcaagtg cggctgaaat tttacgaaag aaggtataac
      361 cggtataaac agaaccttcg gcacgtatac ctgttttcca ttcaccataa tctacggtat
      421 ctggtaccag tgcccagttc aggctgttta caaaggccgt gccaaaaaag gcgacacaag
      481 agaacatgac gaaagtgaat gagttactgc cccagcaata attcaggata tcccccaccg
      541 cccacagcac catgccagcc agataaacct gttttttacc aaaacatttt acagttagtg
      601 gcaccagtaa tacgccgacg agaatgcatc ccatactaaa gaatcccatc cacgacaata
      661 agttaatatc attcagcaca tactgggtgt aatagacctg aatcgccagt ttgatattaa
      721 atgccgccag ggtacacagg ttagcaatgc acaataccag caaaggtgga ttactaaata
      781 ttgcgtaaaa tgatttgaga atactggcct tatgtccggt tggcgtcgcg tccacataat
      841 gctctttgac acctctgtag cacagcatca taaaaataaa gccgccaatg gagaacatca
      901 gtgccgcgca ggcgtagccg acggtggagt cagaaaatag tgactgcaaa ggaataaacg
      961 ccacggtaca gatcaatagc cctatggttg caccaccctg gcggtaagca gcgagttgcg
     1021 cgcgctcatt cgggtttttg gtaatggccg ggatcatcgc gccatacgag cagttcatca
     1081 gactgtatga aagtccaaac atcataaaca gcgcggtggc aatcgtcgtt ttaaccggca
     1141 aactaaaagt ggtggcgata aactggagcg ttgcaattaa cgccgccgga atcgctgcgt
     1201 ataaaataaa cggcctgaat tttcctttag gtccaatatt tttccgcgag tcgagtaaaa
     1261 atccggtgag catatcggta aatgcggtaa agaatttcgc caccagaaag ataatcccgc
     1321 catagtaagc aggcatccct aattcatcgg tataaaattt gaggagatac agagtgccga
     1381 tgctaagcat taaattcgaa ccgacgtcgc ccataccata ggcgattttt tcccgcagat
     1441 tcagttttaa tgtcagtgga ttatggtcag acataatcac tccttataac gccccgcagg
     1501 gcgttgttta ttataattac gccgtgcgtt tacgggcttc tatctcttca acaatgcgga
     1561 cgtacatctt ctcgttcaga ctgtagaagc aacccattgc cacaatggtg actactgcca
     1621 gtgcgcttgg gtagatgaag atcagttggc gcaacccttc gatagtgtgg tcagcctggg
     1681 cgacgtttgg cacatagcca atttgcgtca gcatccagcc tgggaagaaa cctgccagcg
     1741 cctgagatac tttgcgaaag aaggtgaaac cggtgtagac cgtaccttcc gaacgcacac
     1801 cggtgcgcca ctcgccatac tcgacggtgt cagaaaccag cgcccagttc aggctgttaa
     1861 caaacgccga accaaagaac gccaggcagg agaaagccac aaagctgacc gagccgccgc
     1921 cgaagaaata gttgagcaga tcgcccagca cccagatcag cagaccgccg atatagacct
     1981 tcttcttacc aaaacgtctg actgcaccag gcatcaggaa cacgccgatg aaaatacagc
     2041 ccatgctgaa aaatcccata tatgacaaca ggatgggatc gttaagcacg tactgggtgt
     2101 aatagacctg aatggcgagc ttgacgttaa acgcccctaa ggtacagagg ttggcaatgc
     2161 acaaaatgaa cagtgggcgg ttccccgcaa ttgcgcggaa agattgcaac aagccaggct
     2221 tttgcgccgg attggtaggt tgggtttcga cataacgctc tttcacgccc gaatagcaga
     2281 tccacataaa cagcaggccg aacagtgaaa acagcgtggc ggcgaagata tagccaagtt
     2341 gctgattacc ctcgataaga ttcataaccg gcacgaatcc caccgtacac agcagcaggc
     2401 ccaatgtagc gccaccctga cgccatgctg ccagcgatgc acgttcgttg gggtttttag
     2461 taatagcggg gaccatctcg ccatacgagc agttcatcat gctgaaaaac agtccgtaaa
     2521 gcataaacag gatggtggcc atcaccgttt taccggtgac atcaaacggt gtgccgacaa
     2581 agttggcgat cgccagcagg gtgaccggaa atgacgcata cagaataaaa ggacggaact
     2641 taccttttgg accgatcttg cgtcgggaat ccaacataat gccggtaccc atatcggtaa
     2701 aggcggtaaa gaattttgaa atcaaaaaga taatgccgcc ataggtgcca ggcagcccca
     2761 gaacatcggt ataaaacttc aaaagataaa gcgtgccgat atccagtagg atgttagagg
     2821 ccagatcgcc gataccgtaa gagagtttct ctttaaaggg caggcgtaga gttgctggat
     2881 cttccgttgt gatgtgactc atcgttgttc tccctttgcc cgtgagtttc cccacgggcg
     2941 gatgtgatta gatgcttttt aacgacgcga acagtgccgc ccattcgcta tcggcgcgat
     3001 aaaagaccgg cggtttaccg atgggcgcat taacggtcac ttccccgccc cggaacgctt
     3061 cacccgtcca ggcatggacc cagttatcct ccggcagata gagcgtccag tcgctgcgac
     3121 cttcttcgtg taccggggcg accagaatgt cgcggcctaa caggtactga tatttcaggg
     3181 tgtaggtctg tgcatcgtct tcgtaatgca ggaacagcgg gcgcataacc ggcaggccgg
     3241 acttcgcatt cagcgcgacg gcctctttca ggtaaggttt cagggtagtg aagacggtgg
     3301 tcatacgggc aaaatgggcg atagtttccg cgtcgccgtc aaactgccag ttgtcgccag
     3361 gacggttacc ttcatgggtg cgcatcatcg gcgtgaaggc gctgaaatcg caccagcgca
     3421 gcagcagctc tttgctgcgc ttcatctcaa acagggtggt gtaaccgcca atatcactgt
     3481 gatgcaggcc atgtccggtc attgccagcg acagcgccgc cgggacgaca gacgccagtc
     3541 catcgtccag actccagtca acgttctggt cgcccgccca catcatggtg gaatattttt
     3601 ggctaccggt ggaaccggcg cgcataaaga agaggatctc gccgagcttg cccgtttctt
     3661 caagggcttc gtagttacat ttcgcccaca gcgcaggcca ggcgttatgc ataatttcgg
     3721 cgctgacgcc gttatgcagg taagtgtcgg tgggcagata ctcgccgaag tcagccatcc
     3781 agccgccgca gccgagttca atcatgttct ttttgatcac ttccttgaac caggcgtagg
     3841 cttccggatt agtgagatcg acaacgccgc cgtagaactc gccaaactcc accagataat
     3901 cgccgccggc ggcatctttt gccagatagc cgcgctttgc cgcttcttcg cagaggtctt
     3961 tatcgctggc aacatacggg ttgatatagg caaggaactg tacgccctct ttgttccact
     4021 gcttgatacg tgaatccagt tgcgggtagt tttcgctgtt ccacttccag ttccacatta
     4081 cgcgtttgcc aaaagaggtc atacgaatac cggaccaatc ctgcgcccag atgccgttga
     4141 ccttcacgcc cgcgttacgc atggtgtcca gtttcttctg gcacacctca gtgccgccct
     4201 gaatgccgag cgttactccg tcataaatcc agtcgggcag ttctggctgg cgtcccagca
     4261 gcgcggttaa tttttccagc agggagatgt atgtatcagc acattcaaaa cgcagtgttg
     4321 ctttgtcttc ccacagcgcc agttcatggt attccggcgc gctaaagtcg aagttcatat
     4381 agcaactgtt atcaacatgg cagtaatact tctgcgtgct aacaaacgtg ggttgtggaa
     4441 agaaggtcca gtagtaatcc cctcccgcat tctctttgca gtcggcctgc caggtgacat
     4501 aggtttgttt gttgcgacca acgccttgtt cactggtcca tagcgggaac ggtttgccgc
     4561 gcagatcaaa gtaggagaat tgttcgccgc agccgtagat atgatcttcc ggctgtgcgg
     4621 caaggcgcag ccagatgcgg ttgtggttaa ggttgtcgtt ttgtagttcc agcaataaac
     4681 ggccatgatc gtcggcagag atattcagcg tggcgctaat gtcagaacca cggctgaaat
     4741 ggattaacca gccatccggt gactggctga cgatggcgtc ggtaagcgca attttctcct
     4801 gcaatttatc tttaatgctg aaattaccgc ggaacatatc gatatccgct atacctgaac
     4861 caatccataa acaaggatta tctttgctat ggattaaaat aaggcggtct tgaaaacgta
     4921 gagtaaaact gtcgttattc tgatgaaatt gaaaatctaa tagctgtggg cgtggcgtat
     4981 ccataaataa tctccataga tataaaacta atatttcatg ccatcctgtt attacaggtt
     5041 tcattaatta aagaatgtgt gcgggtataa attaaaaccc gcataatgat tatttacttt
     5101 cttcagcccg acggattgcc gccacttttt taccaaatgc cagttgcagt ataactaaca
     5161 aggtaaacgg aggaagaagt gcggcagcgg cgggaattcc cagcgcgcct tttatcaaca
     5221 tagaaccgga ggcaatcagc aataaaaaga ccatcacacg gaacactttg accgaaatgc
     5281 gtttatgcac atattgtcca accagtgaac cgaggatcat ggtgggaata caaaacagca
     5341 ccagtttaaa gatagagaca gtcaaaattc cgctggtcgc cagcccgccg atcaccgcga
     5401 tgttgtttgc ggtgaagaaa gcgttcagag tgccgcgaaa tgccgccggg cctaagttac
     5461 gcagcatacc gtagataacc atcggcggtc cgttggttga aaatgctgcc cctaacgctc
     5521 ctgccacggc gccgataggt gcggcgatcc agctacggtc ataaatcggc aggcgtggaa
     5581 taaacatact gtaaaaggag tacaggataa ggaatccgcc taagcccact ttcataaaat
     5641 ggtcgggtaa atacgacagc gcatataaac cgacgggtat tcccagaaac gagaaaataa
     5701 tcagtaccat tgccgatcgc caatccgttt ctttacgtga cagccaggtg gcgtacaagg
     5761 cggtcgcggt gccgacaatc accgagagcg gagttgccat ttttacgggg agcattaacg
     5821 tgacgagcgg catggtggtt aaaccaccgc caaatcccgc gcatatcccc acaaaggtat
     5881 aaagaaacat taacgcgata acgatacata ccacgtttgt ttccagcatg accccatccg
     5941 gtgaaaataa cgtaaaaaag tccataatat ttcctgttgt gtaaatacaa ctctaaacaa
     6001 tgatctcgtt cttttcttaa taaaattaat tgtgctgccc gtgaatatta aaaaacaggc
     6061 ggtgcgtttt ccctggttcc agtaataata agtcgatacc tgaattaaag gcgttaggcg
     6121 ggcaactcat tggttcaact gccagtccct gacgttgtaa tttttcaccg ctataaacct
     6181 gcacccaggg ttgatcggaa cagaggctca ccgaaagcgc ctgctgtgga tgggtaatag
     6241 tcatttccca caaatcatta gcggttttaa aggtgtggtc gatttttgtc gcggctattt
     6301 ttttcgcctg cgtgaaattt aaatccagct catcaacgtg gtgaagcgtt gtcggattag
     6361 cgtgctcatc gatggcataa acttggtttg ccggaagctg gaacagatat tcatcaacag
     6421 atgttaagtt gcaggttaaa taagggtgaa tacccacacc gtaaggcgct gcgacagtgc
     6481 cgatattctg gcttgcgatc tcaacggaaa gacccgtgcg ggcgttgagc gaataaacca
     6541 cctgcgaagc cagcataaac gggtagccgt aactgggtgg cagaaaggcg gttaatgtga
     6601 cgctggtggc gcttaattcg ctgatttgcc agtcacgcca ggcgagcagc ccatgaatag
     6661 cggcttttga accatgttca ttaatgggta actgatattc ctgcccctga tagcgataac
     6721 agccgttggc gatccggttt ggccacggaa taaggacttt gcccagatgt gccagcggca
     6781 tctcttcggg tttatgcgga ataactaaat gacagccctg aaaggttaat tcagccagac
     6841 ctgcaccgac ggtcacaatg gtcgcgtgat aatcccccgc ggcaagtgaa acggtctgtc
     6901 cactgcaatg catgttggtg atttgcatac gaggtacctt tattgaataa cgacataccc
     6961 ggcgtgcagc ctgatatgtc gtttttttag tcagttattt cgcgttaata tccagcagac
     7021 ccgccgcaac cgccggagcc aggcctggcg ctaacgggat acgtgggatc accagacaat
     7081 gcagcagatg ataaatatcc tgtttgccgt cccagacttt ggtggtgacc ttattgtccg
     7141 catccagctc ctgccaccat gaaccatttt cgtagtccat caggtatttg atgcagtagt
     7201 cccaccatgt ctgataccaa gtttcgtact ggcgatcgcc agtgacggtg tagagcgcgt
     7261 aggccgtacc catcgcttcg acgataggcc aacgcacacg ttcgcggacc accggttttc
     7321 cttcccagtc aacggtataa acaatcccgt ccgcgccatc gggtgcccag gcatcgcgca
     7381 cggtggcgtt aaacagacct ttggcatctt ctagcagcca tgctggcggt tgttcgcaac
     7441 gggcttccag ggccgcgtga atgtgcagca ttaaacggcc ccattcgatc cagtggcccg
     7501 gagtacctcc gaacgcgcgg aagcggtgcg ccgggttatc tttgttgtaa tctggcagcg
     7561 gattccactg ggtatcgaag tgttcgttaa cgcgataatg attatttctg gcgacgtcgt
     7621 ggataatcac ggaagccacg cgaatcgcgc gatccagcca ttttttgtca tgggtgacgt
     7681 catagacaat caggaaggct tccaccgcgt gcatattggc attgccgccg cgatactctt
     7741 cggttttgct gaaggcttca tcccaggact ccaggcacat ctgctcttct tcgctccaga
     7801 agtatttctc gataatttca atggtgtaat cgagcagctt gcgcgcttcc ggatgaccgg
     7861 tagtgacggc gctggcagca cccagcagag caaagaaatg ttgatagccc tgtttggagg
     7921 catccaccac gccctcgtca ttcacgcagg cataccaacc gccatatttt ttatcgcgca
     7981 gtgcgccatt catggctttg ataccgtgat caaccaacga gtaagcgcca ggtcggccca
     8041 tcgccgcagc aacggaataa acgtgcaaca tacgagctgt gatccacaga tgggtgccca
     8101 tctcttcttt gatttgccct ttattgccta accagccaaa tccggtcggt actacggatt
     8161 ttttaccaaa atcaaagatg cgatcggttt cctgttccag ccaacggttg tgacttaaag
     8221 tgttaaacca tttcattttt gtatccccat cttaacggcg tttagccatc atttcgtcga
     8281 cgatatcgcc aagttgttgt aatttcggtg cgcaaacgtc acgcagcatc agctcgttgt
     8341 ctggtaaacc gacgaccgat gcccagacgg cacgaccagc caggaagccc gatgcacccg
     8401 ctgtcattgc cacgcgtacg gcacgcggga acagtttttc gtcgacaccg gaagagagga
     8461 tcacccacgg catattgata tggtcattca gacgttgtga agcactgaga agctcttgtt
     8521 gcggaccttt accgtaaagc ggcatttcaa ctttgtagag atcagcgcca ctgtcgccca
     8581 gctctttggc tgcatcgatg attgcttgtt cgcgatcgaa tttatcgcca cgacgcggtg
     8641 ggcggacgac tggctcaatg atgcttacca gaccgtgtga gtggcacatt tcgttaaact
     8701 ctttcaccat atccaggcgt tgctgcgcat cttcatcact acgccacagc accagcagtt
     8761 ttaaggcttt gccaccgtct tgtttgattt gtagcggatt gattttgcgg tcaatgacca
     8821 cgctatctac cggaataccg ttgccaggaa tgaactcatc ggcggcgaca atcatggcgc
     8881 aacttttggc aatcgcgttt tgctcaacca cctggcggta gcagaattgt tgatctacca
     8941 gaatcgccga tgcataaggt gaaagagtct tcgcggcgtt aactttgaaa tcagttaaaa
     9001 cgctatcggc tactggagcg ggtgcgccag ccgcggcaaa catcatgcgc atggcttcgc
     9061 gctgatcgac cgccagcatg gcaaaaccgc ccgatgcacg cgtaatgtcg ttgatggtgt
     9121 acttattcat tcatttattc ctttatccag tcgttacatt tttactttgg cagtcatccc
     9181 ggcactgaca cggacctgct ccagaatggc ggaccagtcc tggcgaccgc gacccgctgc
     9241 gcgcgcctgg ctataaacct cccgtgaggc ggcccccagt ggcatcggca catgcaactg
     9301 gttggcgaca tcaagggcga tgccaagatc cttatgggca agatcgatca tgaaggcggg
     9361 agaaagatct ccgctgagga ctttgttcgg ccaggaagtg gtgaagtggc ctttaccggc
     9421 ggcagtaccg ctcattacct tgacggcaac atcgaaggga agattgaggg cttcgcacaa
     9481 aacggcggct tctgccgaca acgcattgag cgcgatgctc atgtagttgt tgataagctt
     9541 aacgcggatc cccatgcccg gaccgcccgc gttgatcaac tcgctgccca tcgccatcag
     9601 gatcggcgtg gcgcgttcaa cttgttcagc agtgccgcca gccagcagta gcagagtacc
     9661 ggtaatggca tttgcggaag tacggcctac cggaacatcc atcatgctga agcctctggc
     9721 ctgcatatca gcaatcaatt tatcggtttg cagtgggtgg atggtcgaca tatcaatgac
     9781 cagcgcatcg gaagataagc tctcacagac accgttttca ccgaacaaca cgtgacgcac
     9841 cagatcgcca ttcggcaaca tggtaatgat aaattcggca cctttagcgg cctgcgctgg
     9901 gttagcagcg ggagtcgccc ctttgtcgac cagatgctgt acggcttcgg tattcacatc
     9961 gaagacgcaa agttgatgcc cttgctgcaa taaattgctc gccattggcg aacccatttg
    10021 ccctaaaccg ataaacgcga ttgctgccat aactctctcc tgaatacagt gatgtcactt
    10081 tttgtcattt atgacatgcc ttgcttgtct gtttttgatc gtatttgtaa tttatcgtca
    10141 aaaaattgac agccgtcact ttttaaacaa ttgatgaaat taaaatgaac gcatcccaaa
    10201 atgcttaagg aatgaccatg attcgtgttg cttgtgtagg aattaccgtg atggatcgca
    10261 tctattacgt ggaaggattg ccgacggaga gcggtaaata cgtggcgaaa aattatacgg
    10321 aagttggcgg cgggccagcg gctacagcag cagttgcggc ggccagacta ggtgcgcagg
    10381 tcgattttat tggtcgcgtc ggtgacgacg acacgggcaa cagcctgctg gcagagctgg
    10441 aatcctgggg tgttaacacc cgttacacca aacggtataa ccaggcgaaa tcttcgcaat
    10501 ccgccatcat ggtggatgcc aaaggtgagc gcatcatcat taactatccc agcccagacc
    10561 tactgcctga tgcagagtgg ttggaggaaa ttgatttctc tcagtgggac gtggtgctgg
    10621 cagatgtacg ctggcacgac ggcgcgaaaa aagccttcac tctggcgcgg caggcgggcg
    10681 taatgaccgt gctggacggt gatattacgc cgcaggatat cagcgaactg gtggcgttaa
    10741 gcgatcatgc agctttttca gaaccgggtc tggcgcgctt aacgggcgtg aaagagatgg
    10801 cgagtgccct aaaacaggca caaacgctca caaatggaca tgtctatgtt actcagggca
    10861 gtgcaggctg tgactggctg gagaatggcg ggcgacagca tcaaccggcc ttcaaagttg
    10921 atgtggtaga taccacaggt gcgggtgatg tttttcacgg cgcattggcg gtggcgctgg
    10981 caacaggtgg ggatttagcg gaatcagtcc gcttcgccag cggagtagcg gcgttgaaat
    11041 gcacacgtcc cggtggacga gctgggatcc ctgactgtga tcaaacccga tcttttttgt
    11101 cactttttgt ataaaatgcc agggtgatgg tttttcgagg aattttcatg agccttaccg
    11161 aactaaccgg taacccgcgg cacgaccaac tcctcatgct gatcgccgag cgtgggtata
    11221 tgaatattga tgagctggca aatctgctgg atgtctccac gcagacggtc cgccgggata
    11281 ttcgtaaatt aagcgagcaa ggactgatta cgcgccatca cggtggcgcg ggtcgggctt
    11341 ccagcgtcgt taatacggcg ttcgagcagc gtgaggtttc gcaaaccgaa gaaaaaaaag
    11401 cgattgccga agcggtggca gactatattc ctgatggttc aacaatattt atcaccattg
    11461 gtacgaccgt tgagcatgtt gcccgggcgt tacttaacca taaccatttg cggataatca
    11521 ccaacagcct gcgtgtggcg catattcttt accacaaccc gcgctttgaa gtgatggtgc
    11581 ctggcggaac gctgcgttcc cataacagtg ggatcattgg cccttcagcg gcgtcctttg
    11641 tggccgattt tcgcgccgat tatctggtaa caagtgtagg cgcaattgag agcgatggcg
    11701 cattgatgga gtttgatgtt aacgaagcta acgtggtgaa aacgatgatg gcgcacgcga
    11761 gaaatattct gctggtcgcc gatcacacta agtatcatgc ttcggcggcg gttgaaattg
    11821 gtaatgtggc acaggtcact gcgctcttta ccgacgagct gccgcccgct gcgctaaaat
    11881 cacgcttaca agacagccaa attgaaatca tccttcccca ggaagacgcg tagaattttg
    11941 tgaccgttga cctggcttca tacctgacca catagccaaa cccatccttt cccgctacag
    12001 ttaatttctt gtggcgcgaa aggaggcaaa aatgctctat atctttgatt taggtaatgt
    12061 gattgtcgat atcgacttta accgtgtgct gggggcctgg agcgatttaa cgcgtgttcc
    12121 actggctacg cttaagaaga gtttccacat gggcgaggcg tttcatcagc atgagcgtgg
    12181 ggaaattagc gacgaagcgt ttgcagaggc actgtgtcat gagatggccc tgccgctaag
    12241 ctatgagcag ttctcccacg gttggcaggc ggtatttgtt gcgctacgac cggaagtgat
    12301 cgccatcatg cataaactgc gtgagcaggg gcatcgcgtg gtggtgcttt ccaatactaa
    12361 ccgcctgcat actaccttct ggccggagga atacccggaa atccgtgatg ctgctgacca
    12421 tatctatctg tctcaagact tgggtatgcg caaacctgaa gcacgaattt atcagcatgt
    12481 tttgcaggca gaaggttttt caccagacga tacggtcttt ttcgacgata acgccgataa
    12541 tatagaagga gctaatcagc taggcattac tagtattctg gtgaaagata aaaccaccat
    12601 cccggactat ttcgcgaagg tgttatgcta aaaaccattc aggacaaagc caggcatcgt
    12661 acccgtccac tatgggcctg gctaaaacta ctctggcaac gcattgatga ggacaacatg
    12721 acaaccctgg caggtaacct tgcctatgtg tcgttgctct cattagtgcc gctggttgcc
    12781 gttgtttttg cgcttttcgc cgcttttccc atgttttccg acgtcagcat tcagttgcgt
    12841 cactttattt ttgccaactt tctgcctgct actggcgatg ttatccagcg atatatcgaa
    12901 cagtttgtcg ccaattccaa caaaatgacc gccgtcgggg cgtgcgggct gatcgtcacg
    12961 gcgttattgt tgatgtactc catcgatagc gcgttgaata ccatctggcg cagtaaacga
    13021 gcgcgaccaa aaatttactc gtttgccgtg tactggatga ttttaacgct ggggccgctg
    13081 ttggcagggg ccagtctggc gatcagctcc tatctgctct ctctgcgctg ggcgagcgat
    13141 ctcaatactg tcatcgataa tgtgttgcgt atttttccgc tgctgttgtc gtggatctct
    13201 ttctggctgc tgtacagcat cgttcctacc atccgtgtac ctaaccgcga cgcgattgtc
    13261 ggcgcgtttg tcgccgcgct cctgttcgaa gcaggaaaga aaggtttcgc gctttatatc
    13321 accatgttcc catcatatca gctcatttac ggtgtgctgg cggtgatccc cattctcttt
    13381 gtttgggtct actggacatg gtgtatcgtc ttgcttggcg cggaaattac tgtcactctc
    13441 ggggaatacc gcaaattaaa acaagctgct gaacaagaag aagacgacga accatgattg
    13501 cattaattca acgcgtaacc cgtgccagcg tcaccgtgga gggagaagtg acgggtgaaa
    13561 ttggcgcggg acttttggtg ttattgggtg tcgaaaagga tgacgacgaa cagaaagcaa
    13621 accgtctgtg cgagcgtgtg ctcggctacc gcatctttag cgatgccgaa ggcaagatga
    13681 atctcaacgt gcaacaggca ggcggcagtg tactggtggt ttcccagttt acccttgctg
    13741 cagataccga acgggggatg cgcccaagtt tctccaaagg cgcatcaccg gatcgcgcag
    13801 aggcgttata tgactatttc gtcgaacgct gccgtcagca agagatgaac acgcaaacag
    13861 gacgcttcgc tgcggatatg caggtatcgc tggtcaatga tggccccgtg acattctggt
    13921 tgcaggtatg agccagcttc cagggttgtc acgggaaaca agagagagta tcgctatgta
    13981 tcaccttcgg gttccacaaa cagaagaaga attagagcgt tactatcagt ttcgctggga
    14041 aatgttgcgt aagcccctgc atcaaccaaa aggttcggaa cgcgacgcgt gggatgcgat
    14101 ggcgcatcac cagatggtcg tcgacgagca gggtaatctg gtagcggtag gccgactgta
    14161 tattaatgcc gacaatgaag cgtccattcg ctttatggcc gttcatcccg acgtgcagga
    14221 caaagggtta ggcacgctga tggcgatgac cctggagtcg gtggcacgtc aggaaggcgt
    14281 taagcgcgtg acctgtagcg cccgtgaaga cgcggtggag tttttcgcca agctggggtt
    14341 tgttaatcag ggtgaaatca ccacgccaac caccacgccg attcgccatt ttttgatgat
    14401 taaacccgtc gccactctgg atgatatttt gcatcgcggc gactggtgcg cgcagctgca
    14461 acaggcgtgg tatgaacaca tcccgctgag tgaaaaaatg ggtgtgcgca ttcagcaata
    14521 taccgggcaa aaatttatca ccaccatgcc ggaaacaggc aatcagaatc cgcatcacac
    14581 gctgtttgcc gggagtttat tctcgctggc aacgctcact ggttgggggc ttatctggct
    14641 gatgctgcgc gaacgccacc tcggcggaac gattattctg gcggatgcgc atatccgcta
    14701 cagcaagccg attagcggaa aaccccatgc ggtagccgac cttggtgcct taagtggcga
    14761 tctcgaccgt ctggcgcgcg gacgaaaagc gcgggtacaa atgcaggtcg aaatctttgg
    14821 cgacgagacg ccgggtgcag tgtttgaagg cacgtatatc gttctgcccg cgaagccatt
    14881 tggcccgtat gaagagggcg ggaacgaaga agagtagggg cgttttactt gactgaaaag
    14941 gttttcaatc tgaaaacctt ttctgggccc tggtccggag ttaccttccc gctctccagg
    15001 caacatcccg tagcgccgat cgcgcagcct cactgatacc acctaactga aaaaatccgt
    15061 ggataacgcc aaggtagcgt tggcaggtgc attgcactcc ttgccctgtc atgcggtgat
    15121 acaacgcttc accttcatcg caaagtgggt cgtattcagc ggtaatgata tgtacaggtg
    15181 gcaagccatt gaaatcattg cgccaaatag ggctggcttc aggatgcctg cgttcaatgc
    15241 ctggcaggta catctcaaag ccgctcagta aagtgtcgcg tgtgatgatg taatcgtgac
    15301 cattgcgtat gtagctttcg aaatgcgcag tcgcatcaag cataggataa attaagatga
    15361 gttgtgctgg ttgccactcg ccagcacgct tcaatcgcaa agcagttacc agcgccagat
    15421 gccctcccgc actgtcaccg gcaagagtta ttcgttgttt atcaacgccg agtcgttcgg
    15481 cgtgttgccg gactaattct gcacctcgtt gtgcatcatc atgggcggcg gggaaagtat
    15541 gttccggtgc cagccgatac tggacggcaa taatccgaca ttgcccgtaa taggctaact
    15601 ggcgcagctg gttgtcgtga gttgcgaacc cgccgctaac aaaacagcca ccgtagtaat
    15661 aaattatcgt cgggagtaat gtcggggcat tcagcggtga cactattcgt aaggtcatcc
    15721 cttcaagttc gatagtctga atatctaccc gcgtttctgt ctcacccgca aggacagcgc
    15781 tggcgatata gccctctctg cgctgcgtaa tactttgttg gcgcgatgag gggcgacccg
    15841 cagcgataaa ctcttcgact aactctgcaa ttcctttttc cagtgccata gtagaactca
    15901 ttttgctgta tgaatgtaca gttttataca cccgtttttg ctggatggat acagggaaat
    15961 tcacgggcgt gaaaaatctg gaaagcgcct cgcaaatgta aagacaaacc cggcagagac
    16021 gtggttttgc tggagcacta tcagtaagct agtggtttca atggtacgtc aatgacgtac
    16081 aaaaaggatt gttactctaa gccaatggcg tataattatg ctcttcatcg aaacggaaat
    16141 ttttactgaa gatgttcaaa aactgctgaa cgacgatgaa ttcagtcgtt ttcagttttt
    16201 cctggccttg aatcctgact atggtgaggt aatacctgaa actggtgggt taagaaaggt
    16261 tcgatgggtc tcaggaggaa aggggaaatg tgctggtgtc agggttattt actttcatca
    16321 ggttaaacat tatgagatca gattactgct tatttatcgt aagggtatta aagatgatct
    16381 gtctccccag gaaaaagcaa tgctccggtt gttaaatacg aggtggtaaa tggataaggc
    16441 actttttgag cggttgactc acagcatggc ccagatgaat gaaattattg aaggaacgcg
    16501 acagccttca cggacttttg aagttgatgc tatgaaaatc aaagaaatac gccgcgcttc
    16561 agggttgtca caatctaaat ttgccgatct tatttccgta agcgtagaca cgttgcgtaa
    16621 ctgggagcag gggcgacgtt caccgaccgg gcctgcaaag gctttgctgc gagcgattgc
    16681 caatgatccg caacatgtgc tgcaagcact taaccgttag aggcatggtt atgccataca
    16741 gaaagcacca gcattcagaa ctcctatgaa agcggcatag tgccgcctgc tcaggagttt
    16801 tgctatgacc cgtaatcgtg ctgaacgtcg ccatcatatg gtgcgattaa aaaaaatccg
    16861 ctgtcgttat cgcactgcag gcgatggaag caaaaaagca tcaggtattt gctttcgtac
    16921 tccctgtatt tgttcttgtt ggatgtgtgg gcatcgacgc tatcataatg gtccccgcgt
    16981 atcggaaatt cgtgcaaaag cacgctatgc gaattaaggg aaaatgatga actattttaa
    17041 ggaagcacat ttttagaact cttaacagga gactaaaaat gtccacgttt aatacgcgta
    17101 aagataggga tcgtaatggt aaagcgaagc cgcataagca ttcagggatc ccaacactta
    17161 caggtaaatg gcgtcaccct tcaatggatt gttgggtaaa ctacagtaca ccgggcgatc
    17221 atgaatggaa gcggctctat actacgcttc gccgtcgcgc agatgatcga cgccaatgtc
    17281 atcgggtaat gcagggggat gatccggaag gaatcgtctg gttcccggac tgttatccac
    17341 aaatttacta ctactaacaa ttaaagaccg gttggtgcgg cactgaccgg tcgttatgaa
    17401 tggtagattt caccttaaac cctggtgcgg taaagctgct gactttttcg ggtacaccat
    17461 cctgatgaag caaatgaaga c
//
LOCUS       DADSZD010000094        17344 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.095, whole genome shotgun sequence.
ACCESSION   DADSZD010000094 DADSZD010000000
VERSION     DADSZD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.095"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            38..556
                     /locus_tag="J1Z28_002837"
     CDS             38..556
                     /locus_tag="J1Z28_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBA7717656.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            572..1111
                     /locus_tag="J1Z28_002838"
     CDS             572..1111
                     /locus_tag="J1Z28_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717657.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1204..2781)
                     /gene="guaA"
                     /locus_tag="J1Z28_002839"
     CDS             complement(1204..2781)
                     /gene="guaA"
                     /locus_tag="J1Z28_002839"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBA7717658.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2850..4316)
                     /gene="guaB"
                     /locus_tag="J1Z28_002840"
     CDS             complement(2850..4316)
                     /gene="guaB"
                     /locus_tag="J1Z28_002840"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004159092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBA7717659.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4478..5854
                     /gene="xseA"
                     /locus_tag="J1Z28_002841"
     CDS             4478..5854
                     /gene="xseA"
                     /locus_tag="J1Z28_002841"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBA7717660.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTHIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLTTRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(5923..13974)
                     /locus_tag="J1Z28_002842"
     CDS             complement(5923..13974)
                     /locus_tag="J1Z28_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RatA-like protein"
                     /protein_id="HBA7717661.1"
                     /translation="MSLTLNKILVLCALLISAMLPGWSWAESAWQDSSDTVGEFNGTV
                     PTADSASIPVYQGSVFLDPTKTHEVAFTAKPSEFNADVSVSKLLVTNPQDREGDIIAT
                     PRWENQTPPAVSLVWADAATPDTLLDPQPVADRSFCAQGLAGRSLVAWAQPDPQQTMP
                     LLYLLTSTGYPYESVLTLADQKVTLKIAPAQGDLISVSAAGYDESSGAAKMTVGGSIT
                     LTVTTKDCVGNVVGNIPFVIKRKDAENRQGVVNNTAPVKLGTTELTTTATEYRGTTDA
                     NGVATVTVTQANGPGVKTPLVASLAGIAQASETAVIFTVLTSPDVPQATMWGHMPDTL
                     KARDYTFSRPKLAAEVDNEGGTVNDHNETWSTFTWSGADKHCDILPGMRQFGALATVV
                     PTSVQDVAGWPMQGNFYWSSLAGMSGQHHAADVSNRSEAQKPDDTTFIVSCVDKEAPD
                     VEPKLVLTPGSYDSTIKAMKVKVGEEASLRLTITDSKNNDQPLAYYYFSLHLDDGINR
                     KNQTDAAWETHPVQIDGGSNVRKVDAHTYEGITDANGEATLTLTQPGGVGVKTHITAR
                     MRSDFTASDEKDVIFTVITSPDTDKARMWGHMLGIIEANNIFKRPRLADETDNELGSV
                     RENNEDWALFDQNSSMQAECGLGHIPSQSSLHSLFAAHPANAIGTEYGWPTLQKAYLS
                     AVEETSHASVNLATGNIDTYSGFKQNYLSCSGNEMVAKIAATTDRDVSAGSRAQAKVG
                     DTITMTVRTFNALNNAPVPYTAFTITKDMGKNRQGQTTGFDDPTRGAIEMNGTLYGTS
                     QPSLVYAGTTDAQGFATVEIKQSQGVGLSTPLNIVPVNSYIPNTVNYNVIFTTLTSPD
                     AVGAQMWGHMDETITVDALTFARPRLAAEVFSPDGTLTENNEVWSRVSQANASSTSKG
                     GCGANMLPRRSQLSALYDANNGDGVQTVHGWPTQRQPYWSSSPADQVPHYYTIALNDG
                     ARTVGGSTAVYVSCLTTANNPASSITLEVVDPAQWNAAANAAKLKKGETLQVKVTVKD
                     AQGNPLGDIPFTLKRGDGYTRSEEKHVAGSGDALVAPVVVNGGLADETSLNNTAAAYS
                     AITGSDGTKILTITRPDTHGTKTSLTATLYSDTTKKATLDTIFTVVTSPDSDKAKMWG
                     HMPETVTAADGTVFKRPLLLKELSSTSGRTAIAEENEDWAQFTQTQAISTSSNGCGSE
                     YVPSQAGLESLYEANRGNAMKTVQGWPVASSYLSSTTGSSSLEQRDFKAVNLSSGTSS
                     IIPSATKELLTCQTTPIVKASQIVLEAADPTKFDSTNNVVKAKKGEEAVLRVTTKDAQ
                     GNPVGNTAFTLKRNTSVNRANVSTTTSIASLAVTDAWGNTQDDFLSTTLVIYGVTGAD
                     GITTFTLKQDQTTGLKTELTAALDSSSSTKSTLPVVFTVLTSPDSPKAKFWGHMAETA
                     TGDDGLIYRRPLLRDENSATTSIGTLVEEGEAWSTFPSGQANDTSINGCGAEYVPTDN
                     ELRAIYAHQGSSALHDAIGWPVSRFYISNTVADTFTQTFTYDVVSLKTGDETQMPSSG
                     GALLSCRTTPVAVASQIIVEANDTAQFVKVDDTLSALKVKKGEDAVIRVVTKNAQGNS
                     VPNVPFILRREGSKNRQNAEMINKSITVINAAGASARMNSSSSLLYGVTGADGTTSFT
                     VKQDDSMGLVTNMYAQLYQLTIESNKLPVMFTVITSPDTPLASYWGHMPETFTTRSGI
                     AFKRPLLTAEHPAGQSTMANNESWLSLNTAAKNDVSKSDCGEPYQPLLSEFQELYSEH
                     PNGAIGTDLGLPLTNTWWAYDKIAYANVWYDQSINLSNGSSSRALSNTVAFVSCLVNP
                     HAVAASIEMTSTALDAEKTASNDGRPSATAAKGTAIRMAVIVRDSAGNLLPGANFNLI
                     RGTALDRAKNRLDSTYDDLTIVPVTPAGVNMSLYNNGAQALLTTGSDGKATFDVTQNE
                     TYGLATPLTATLMRDTTKSATMDVIFTVITSPNSPKAKYWGHMPDTFTSRAGVTFKRP
                     LLAAEATLGSSVSNNNESWSYLFYTNKVTPDCPVEYQPRLNELQGLYNDHPGGTILTD
                     LGLPITAGSGNWWTYEMSTTDALTWYYGVINLKTGQSTTTINGYALMLCLTQPHSAPA
                     SLTLSSTAYDEGRTASNGGTPTSSVKKGEMLPIVVTIKDANGNPVGGEGVTLKRVQAK
                     SRSGISVSSNTVDDLILDEVTPTSARISFNQNTSAWSGFTGSDGTITFNVTQNNTVGL
                     VTPFTASLARNPQVTANQDLIFTVVTSPDSAKANYWGHMPATLTAVNGAVFERPKLWS
                     ELTSTSGVGKINNNNEDWPYFTPTQKSDASVSPCEVARQPLFNDLSSLSARYPNNTFV
                     TETGWPAYYTWWAEDKSADGKDQSVDLRNGTLYTGSTKSFQPCLANARSTVSSVTLTS
                     TAFDADSQAAKVKKGEAMSVTVTVKDSAGNTVPNVEFTLKRGEASPRNAGATLYGNVV
                     AMDDLIVQPLSGSAITLSESGNTISGMTGADGTASFTLRQDNTPGYKTPLTVTLANYA
                     SATDTLDAIFTVPTSPNVSSAHFWGHMADTVVVNGKSLHRPLLTTELPSGANPVSSPI
                     INYENWASAHIIDASKWDIARQCGSIENAPTYNELELLHTVFNSLGWPSSPSFPYLSS
                     QQCGMDEGTGAQDCSITLINKPGLVTCFQ"
     gene            complement(14095..15072)
                     /locus_tag="J1Z28_002843"
     CDS             complement(14095..15072)
                     /locus_tag="J1Z28_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717662.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELVTKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTASGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(15127..17304)
                     /gene="sinH"
                     /locus_tag="J1Z28_002844"
     CDS             complement(15127..17304)
                     /gene="sinH"
                     /locus_tag="J1Z28_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HBA7717663.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLVSEAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLYVTLWLKADVTNEHPECVIKDTPEEAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDKAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNTYRFVLWRDNNKDGVFQQVEKLTDEEMVQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         4088 a         4593 c         4318 g         4345 t
ORIGIN      
        1 aatgtctcat ttttcgtaat gtgtataaca aggaatagtg atgaaattta aaaaatgtct
       61 tctgcctgtg gcaatgttag cgtcattcac tctggcagga tgccagtcaa atgctgatga
      121 tcatgccgcc gatgtttatc aaaccgatca actgaatacc aaacaagaaa ctaaaaccgt
      181 taatattatt tccattcttc ccgcaaaagt tgccgtagac aacgcccaaa ataaacggaa
      241 cgcacaagcc ttcggcgcgc ttattggcgc tgtcgctggc ggtgttatcg gccacaacgt
      301 cgggtctggc agcaattccg gaacgacggc aggtgcagtt ggcggcggag ctgtaggcgc
      361 ggcagcgggt tctatggtga atgataaaac cttagtggaa ggtgtttctt taacatataa
      421 ggaaggcacc aaagtgtata cctccaccca ggtgggtaaa gagtgccagt ttacgacagg
      481 tttagccgtt gttattacca cgacgtataa cgaaacgcgt attcagccaa ataccaaatg
      541 tcctgaaaag agctaataat caggaggagt catgaagaaa gtttttcttt gcgccatctt
      601 agcctcctta agctatccgg ctatcgcctc atcattgcag gatcaactct cggctgtcgc
      661 agaagcggaa cagcaaggta aaaatgaaga gcaaaggcag catgacgaat gggtcgcgga
      721 gcgcaacagg gaaatccagc aagagaagca acgtcgcgca aacgcccagg ccgcggctaa
      781 taaaagagcg gcaacggcag cagcgaataa gaaagctcgt caggataaac tggacgccga
      841 agccactgcg gacaaaaaac gcgatcaaag ttatgaagat gagctacgta gcttagagat
      901 tcagaaacaa aaactggcgc tggcgaaaga agaagcccgc gtcaagcgcg aaaacgaatt
      961 tatcgatcag gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat ctgaagctga
     1021 cgcaaacaga aatatgactg aaggcggtcg cgatctgatg aaaagcgtgg gcaaagcaga
     1081 agagaataaa tcagatagct ggtttaatta atcgatgtta gtaacttcaa tcctatgatt
     1141 cgtgagtata aaaaaccctc tgtagtaaca gagggttttg ttcattcata gtgcagagtc
     1201 aaatcattcc cactcaatag tcgctggtgg cttgccgctg atgtcataca ccacgcggga
     1261 aataccattc acttcattga taatgcggtt ggaaacgcga ccgaggaaat cgtacggcag
     1321 atgtgcccag tgtgcggtca taaagtcgat ggtttcgaca gcacgcagag agacaaccca
     1381 gtcatactta cgaccatcgc ccattacgcc aacggaacgt actggcagga agacggtgaa
     1441 cgcctggctg actttgtcgt acaggtccgc tttacgcagt tcttcaatga agatggcgtc
     1501 agcacggcgc agcaggtcac aatactcttt cttcacttcc cccagaacac gaacgccaag
     1561 gcctggtccc gggaacgggt gacggtacaa catgtcatac ggtaggccca gctccagacc
     1621 aatcttacgc acttcgtctt tgaacagctc tttcagcggt tcaaccaggc ccatcttcat
     1681 ctctttcggc aggccaccca cgttgtggtg agatttgatg acgtgtgctt taccggttgc
     1741 agaagcagca gattcgataa cgtcagggta gatggtgccc tgcgccagcc acttcacgtc
     1801 ttccagtttc agcgcttctt catcgaatac ttcaacgaaa acgcgaccga tgattttacg
     1861 ttttgcttcc ggatcgtttt cgccagccag cgctgacaag aagcgatctt ctgccggtac
     1921 gtgaacaata ttcagaccaa agtgatcgcc aaacatatcc agaacctgct ctgcttcgtt
     1981 gagacgcagc aggccgttgt cgacgaatac gcaagtcagg tttttaccga tagcgcggtg
     2041 caacagcatt gcggttacgg aggaatccac gccaccagag aggccgagga tgactttatc
     2101 gtcgcccact tgctcgcgga tgcgggctac agcgtcgtcg ataattttcg ctggcgtcca
     2161 cagggcttca cactggcaga tatcacgcac aaaacgctcc agcatgcgca taccctggcg
     2221 ggtgtgagtc acttccgggt ggaactgtac gccatagaag cgtttttctt cgttggccat
     2281 gatggcaaac gggcagcttt cggtgctggc tacggtgacg aagtcggacg ggatagcggt
     2341 aactttatcg ccgtggctca tccagacgtc cagcagcggt ttaccatctg cggtcagcgc
     2401 atcttcgata ccgcgaacca gtgcgctgtc gtttacgact tcaacctgcg cgtagccaaa
     2461 ttcacgttca ttagaagcct caacgtgacc gcccaactgc atcgccatgg tctgcatacc
     2521 atagcaaacg ccgaataccg gtacgcctgc ttcaaagaca tactgcggcg cacgcgggct
     2581 gttttcttca gtagtacttt ccgggccgcc ggaaagaata atgccgcttg gattgaagtc
     2641 acgaatttgt gcttctgtca catcccacgc ccacagttcg cagtaaacgc ccagctcacg
     2701 cacgcggcgc gcaaccagtt gagtatactg ggaaccgaag tccagaatga ggatgcgatg
     2761 cttatgaatg ttttccgtca ttgacgctta ttccgaggca agtgaaacag ataatataaa
     2821 tcgcccgaca tgaagtcggg cgaagagaat caggagccca gacggtagtt cggggactct
     2881 ttagtaatgg tcacgtcgtg aacgtggctt tcctgaatgc ccgcaccgct gatacgtaca
     2941 aactccgctt tagtacgcag ttcgtcgata gtaccacagc cggtcaggcc catacaggag
     3001 cgcaggccgc ccatctgctg gtgaatgatc tctttcaggc gacctttata ggctacgcgg
     3061 ccttcgatac cttccggcac cagtttgtcg gcagcgttgt cgctctggaa gtaacggtca
     3121 gaagaacctt tggacatcgc gcccagggaa cccataccac ggtaagattt gtaagaacgg
     3181 ccctggtaga gttcgatttc acccggagat tcttcagtac ccgccagcat ggaacctacc
     3241 atcaccgcgc ttgcgccagc ggcgatagct ttagcgatat cgcctgagaa acggataccg
     3301 ccgtcagcga taaccggaat gccggtacct tccagggctt ctactgcatc agcaacggcg
     3361 gtgatctgcg gaacaccgac accagtcacg atacgcgtcg tacagataga accagggccg
     3421 atacccactt taacggcact gcaaccggct tctgccagcg cgcgggcacc tgcagcagtt
     3481 gccacgttgc cgccgatgat ttgcaggtca ggatatttag cgcgagtttc acggatacgc
     3541 tgcagaacac cttcagagtg accgtgagag gagtcgatca gcagaacgtc aacgcctgcg
     3601 gcaaccagcg catcaacacg ctcttcgtta ccagcgcccg cgccaaccgc tgcacctaca
     3661 cgcagacggc cttgttcgtc tttacaggcg ttcggtttac gttccgcttt ctggaagtct
     3721 ttcacggtga tcatgccgat caggtggaat tcgtcatcaa ccaccagcgc cttttcaacg
     3781 cgtttttcgt gcatttttgc cagcaccact tcacgggctt cgccttcacg cacagttacc
     3841 agacgctctt tcggcgtcat gtaaacgcta accggttgat tcagatcggt cacaaaacgc
     3901 acgtcacgac cggtgatgat gccgaccagt tcgttttctt cggtaacgac cgggtaaccc
     3961 gcaaagccgt tacgttcagt cagttctttc acttcgcgca gcgtcgtggt tggcaaaaca
     4021 gtctgcgggt cagttactac gccagattcg tgttttttca cacggcgaac ttcttctgcc
     4081 tggcgttcaa tggacatgtt tttgtggata aagccgatac cgccttcctg agccagagca
     4141 atagccaggc gcgcttccgt tacggtatcc attgctgcgg aaagcatagg gatattcaga
     4201 cgaatagttt tcgtcagctg agtgctgagg tcagcagtat tcggcagaac ggtagaatga
     4261 gcaggaacga ggagaacgtc gtcaaacgtc agagcttctt tagcgatacg tagcatgggc
     4321 aatatctcga ccagagtggt taataaatat tgccgcggca ttatacagag cgtaaccgat
     4381 tgcatctacc cctttttgca aaaaatgctt gctatccccg gagggcgggt tactatcgac
     4441 tgaataacct gctgatttag aatttgatct cgctcacatg ttaccttctc aatcccctgc
     4501 aatttttacc gttagccgcc tgaatcaaac ggttcgtctg ctgcttgagc atgagatggg
     4561 gcaggtgtgg atcagcggtg aaatctctaa ttttacacaa ccagcctccg gtcactggta
     4621 ctttacgctt aaagacgata ccgcgcaggt gcgctgtgcg atgttccgca acagcaaccg
     4681 ccgggtcacc ttccgcccac agcacgggca acaggtttta gttcgcgcca atattacact
     4741 ctacgagccg cgcggcgatt atcaaattat cgtcgaaagt atgcagccgg ccggtgaagg
     4801 attgctgcaa cagaagtacg aacagcttaa agcgaagttg caggctgaag gtttgttcga
     4861 tctgcaatac aaaaaatcac tcccctcccc tgcgcattgc gttggtgtga tcacctcaaa
     4921 aaccggtgct gcgctacatg atattttgca tgtgttaaaa cgtcgcgatc cttctctgcc
     4981 agtgattatt tatcccaccg ccgtacaggg ggatgatgcg ccggggcaaa tcgttcgtgc
     5041 cattgaactg gcgaataagc gcaacgagtg tgacgtgttg atcgttggcc gtggcggtgg
     5101 ttcgctggaa gatttatgga gttttaatga tgagcgtgtg gcgcgggcga tttttgccag
     5161 tcgcattccg atagtcagcg ccgtcgggca tgagacggat gtgacgattg ccgactttgt
     5221 tgctgatctg cgtgcgccaa cgccgtcagc cgccgctgaa gtggtgagcc gaaatcagca
     5281 agaattgcta cgtcaggtgc aatcgaccca tcaacggctg gaaatggcga tggattatta
     5341 tctcgccaac cgcacgcgtc gttttacgca gattcatcat cgcttgcagc aacagcatcc
     5401 gcagctccgg ctggcacgcc agcaaactat gctcgaacgt ctgcaaaagc ggatgagctt
     5461 tgcgctggag aatcagctta aacgcgcggg tcagcaacag cagcgattaa cacggcaact
     5521 cgtccagcaa aatccgcagt cgcgtattca tcgcgcgcaa acgcacattc agcaactgga
     5581 atatcgttta gcagaaaccc tgcgcgcaca gcttagcgcc acgcgtgaac gtttcggtaa
     5641 tgcagtaaca cacctcgaag ccgtaagccc actgtcaacg ctggcgcgcg gttatagcgt
     5701 caccagcgcc gctgatggcg cggtgttaaa acaggttaag caagtgaaag tgggtgagac
     5761 actgaccaca cgactgggag atggtgtggt gattagtgaa gtcagcgccg taacaaaaac
     5821 ccgcaaatca cgtaaaaaaa cgtctaatcc gtagaccgga taagaggcgc cagcatcgca
     5881 tccggcaata actcccaggc tacgccagat ccgccatgct ggttactgga agcaggtcac
     5941 cagacccggt ttatttatta gcgtgatact acaatcctgt gctccagtac cttcgtccat
     6001 cccacactgt tgagaggaca aataaggaaa actgggtgaa gacggccatc ccagtgaatt
     6061 aaaaacagta tgcaacaact ctaattcatt gtatgtcggt gcattttcta tgctgccaca
     6121 ctgccttgcg atatcccatt tactggcatc aatgatatgt gcagaagccc agttttcgta
     6181 gttaataatc ggtgaggata cgggattagc cccggacggc aattctgtcg ttaacaacgg
     6241 tcgatgtagc gatttaccat tcaccactac cgtgtctgcc atatgtcccc aaaagtgcgc
     6301 cgaggaaaca tttgggctgg ttggtaccgt aaaaatagcg tccagcgtgt ctgtcgcgga
     6361 agcgtaatta gccagagtaa ccgtcagtgg cgttttataa ccaggggtat tatcctggcg
     6421 gagcgtaaaa cttgccgtcc catccgcgcc tgtcatgcca ctaatggtat taccgctttc
     6481 actcagcgta atagccgaac cagataacgg ctgaacgatg agatcgtcca tcgccactac
     6541 attgccatac aacgttgccc cagcgttacg cggcgatgcc tcaccacgtt tcaaagtaaa
     6601 ttcaacattc ggcaccgtat tcccggcgct gtctttcacc gtcacggtaa cggacatggc
     6661 ttcgcctttt ttcactttcg ccgcctggct gtcagcatca aacgctgttg aggtcagtgt
     6721 gacgctggaa acggttgaac gcgcgttagc caggcaaggc tgaaatgatt ttgttgaacc
     6781 cgtatacagc gtgccatttc tcaaatcaac cgactggtct ttaccatcgg cggatttatc
     6841 ctctgcccac caggtgtaat acgcaggcca gccagtttca gtgacaaacg tgttattcgg
     6901 ataacgcgca gacaacgaac ttaaatcatt gaatagtggc tgccttgcta cttcacaagg
     6961 gctcacactc gcatctgatt tctgtgttgg ggtaaagtac ggccagtctt cattattgtt
     7021 attgatcttg ccgacgccag aagtcgatgt cagttcactc cacaatttcg ggcgttcaaa
     7081 taccgcacca ttcactgccg tcaaggttgc tggcatatgc ccccagtagt tagccttcgc
     7141 agaatccggg ctggtcacta ccgtgaatat caggtcctga ttagccgtaa cctgcggatt
     7201 tcgcgccaat gaagctgtaa acggcgttac cagaccaacg gtgttatttt gggtaacgtt
     7261 gaacgttatc gtgccatcac tgccggtgaa gccgctccat gccgacgtat tctggttgaa
     7321 ggagatacgc gccgatgtcg gggtaacttc atctaaaatg agatcatcta cggtattgga
     7381 ggatacagag atccctgaac ggcttttcgc ctgcacgcgc ttgagcgtaa caccttcgcc
     7441 gccaacagga ttaccattgg catctttgat cgtcaccacg atgggaagca tctcaccttt
     7501 tttcacagat gacgtggggg taccaccatt gctggcagtt cggccctcat cataggcagt
     7561 cgatgacagc gtaaggcttg ctggcgccga atgtggctgc gtcagacaga gcattaaagc
     7621 gtatccattt atagtcgtcg tcgattgtcc cgttttcaaa ttaatgacgc cgtaatacca
     7681 cgtcaacgcg tcagtggtgc tcatttcata agtccaccaa tttccacttc cggcagttat
     7741 aggcaacccc aaatctgtca gtattgtccc gcctggatga tcattatata gcccctgtaa
     7801 ctcattgaga cgcggctgat actcaaccgg gcaatccggc gtaactttat tggtataaaa
     7861 aaggtatgac cacgactcat tattattact taccgaggac cccaaggtcg cttctgctgc
     7921 cagtagcggt cgtttaaagg tcacccccgc cctactggta aacgtgtcgg gcatatgacc
     7981 ccagtatttt gctttcggtg agttcggact ggttatcaca gtgaaaataa catccatcgt
     8041 ggcacttttt gtggtgtcgc gcatcaatgt tgcagtgagc ggagttgcca gcccataagt
     8101 ctcgttttgc gtcacatcaa aagtcgcttt accatcactt cctgtcgtca gaagtgcctg
     8161 tgccccatta ttatagagtg acatgtttac tccggcaggg gtgaccggca cgatagtaag
     8221 atcatcatac gtagaatcta gcctgttttt tgcacgatcc aacgcagtcc cgcgtatgag
     8281 attaaagttt gcgcctggca acagatttcc tgcgctatcc cgcacaatca ccgccatccg
     8341 gatcgccgta cctttcgccg ccgttgcact agggcgacca tcgttgctgg ccgttttctc
     8401 cgcatccagt gccgtggagg tcatctctat agaagctgcc actgcatgag ggtttaccag
     8461 acaggacaca aatgccacgg tgtttgataa tgccctggag cttgatccat tcgataaatt
     8521 aatagactga tcgtaccaga cgttcgcgta ggcaatttta tcgtaagccc accaggtatt
     8581 agtcagcgga aggcctaaat cggtccctat cgcaccatta ggatgttcac tgtataactc
     8641 ttgaaattcg cttaataacg gttgataagg ttcgccgcag tctgatttgc tgacatcatt
     8701 ttttgctgcc gtattcaaag aaagccatga ttcattatta gccatggttg actgccccgc
     8761 tggatgctcg gcagtaagca atggccgctt aaaggcaatt cccgaccggg tagtaaacgt
     8821 ttcaggcata tgcccccagt aacttgccag tggcgtatcc gggctggtta tgacggtaaa
     8881 catgaccggt aatttgttgg attctatcgt cagctgatac aactgggcgt acatattggt
     8941 taccaggccc atagaatcat cttgttttac cgtaaaggag gtagtcccgt ccgcgccagt
     9001 caccccataa agcaggcttg aagaactatt catcctcgca gaagcgcctg ccgcgttaat
     9061 taccgtaatc gacttattta tcatctcggc attttgacgg ttcttcgagc cttcacgtct
     9121 gagaataaag gggacattgg gcacgctatt tccctgcgca tttttagtca ccacgcgaat
     9181 caccgcgtct tcgccttttt tcactttcaa cgcactcaag gtgtcgtcaa ctttgacaaa
     9241 ttgcgccgta tcattcgcct caacaatgat ctgactggca acggcgacag gcgtggtccg
     9301 gcagctcagt aatgcaccac cagatgaggg catctgcgtt tcatctcctg tcttcagcga
     9361 aacgacatca tacgtaaagg tttgagtgaa cgtatctgca acggtattcg agatatagaa
     9421 cctcgacact ggccagccaa tagcatcatg taatgcgcta ctcccctggt gcgcataaat
     9481 ggccctaagt tcattgtcag ttggaacata ctccgcgcca caaccattta tcgaggtatc
     9541 gttagcctgg cccgacggga acgtagacca ggcctccccc tcttccacca gggtaccgat
     9601 actggtggta gcactattct cgtccctcag taaaggcctt cgataaataa gaccgtcatc
     9661 gcctgtcgcc gtttcagcca tatgccccca gaatttggct tttggcgaat ccgggctggt
     9721 aagcaccgta aagacaacgg gaagcgtcga tttagtgctg ctggaggaat ccaacgctgc
     9781 cgtgagttct gtttttagcc cggtagtctg gtcttgcttc aatgtgaaag tggtgatgcc
     9841 atcagccccc gtcacgccat aaataaccag ggtcgttgaa agaaaatcat cttgagtatt
     9901 tccccaggca tccgttaccg ccagcgaagc aatcgacgtc gtagttgaca cattggccct
     9961 gttcacgctg gtgtttcgtt tgagggtaaa tgcggtattt cccacagggt taccctgcgc
    10021 atctttagtg gtcactcgta atacggcttc ttcgcctttt ttcgctttga caacattatt
    10081 cgtactgtca aattttgtgg ggtctgcagc ttcaagaaca atttggcttg ccttaacaat
    10141 cggcgtcgtc tggcaggtaa gcagttcttt tgtggcagat ggaattatcg aactggtgcc
    10201 tgaactgaga ttgacggcct tgaagtcacg ctgttccagg ctactactac ctgtcgtgct
    10261 ggaaaggtaa cttgaggcga cgggccagcc ctgaaccgtt ttcatcgcat tgccgcggtt
    10321 ggcttcatac aatgactcca gcccggcctg ggagggaaca tattcactac cacaaccatt
    10381 agaggatgtg cttatagcct gggtttgggt aaattgcgcc cagtcttcat tctcttctgc
    10441 aatcgctgtt cgacccgacg tactggataa ctctttcaac aataatgggc gtttaaagac
    10501 cgttccatct gcagccgtca ctgtttcagg catatgcccc cacattttcg ccttatcgct
    10561 gtccggactg gttacaacgg taaaaatggt atcgagggtc gcttttttcg ttgtatctga
    10621 atagagcgta gcggtcagcg acgttttcgt gccatgggta tccgggcggg taatagtgag
    10681 aattttagtt ccgtcgctgc cggttatggc gctgtatgct gcagcggtat tattaagcga
    10741 agtttcgtcc gccaggccac cattgaccac aaccggcgca accagcgcat caccgctacc
    10801 agcaacgtgt ttttcctctg agcgggtata accatcgcca cgtttcaggg taaagggtat
    10861 atcacccaat ggattgccct gcgcatcttt taccgtcacc tttacctgta gcgtttcgcc
    10921 ttttttcagc ttcgccgcat ttgcagccgc gttccactgc gccggatcca ccacttccag
    10981 cgtaatgcta ctggcaggat tgttagccgt cgtcaggcaa ctgacataaa ccgccgtgct
    11041 accgccaacg gtccgcgccc catcgttgag ggctatggtg taataatgcg gtacctgatc
    11101 tgcgggcgaa ctgctccagt agggctgacg ctgcgttggc cagccgtgta ctgtctgtac
    11161 accgtcaccg ttattggcat catagagcgc gctaagctga gaacggcgtg ggagcatatt
    11221 ggcgccacag ccacctttac tggtgctgga ggcgttggcc tggctgactc gcgaccagac
    11281 ttcgttattt tccgttaatg tgccgtccgg actgaacact tccgcagcca gtcgggggcg
    11341 ggcaaaggtt aaagcatcga cggtaatcgt ctcatccata tgcccccaca tctgcgcgcc
    11401 aaccgcatcc ggactggtga gcgtagtgaa aatcacattg taattaacag tattgggaat
    11461 ataggagttc acaggcacaa tatttagcgg cgtcgataag cccacacctt gcgattgctt
    11521 aatttccaca gtcgcaaaac cctgcgcatc cgtagtaccg gcatagacta aagacggttg
    11581 cgatgttccg tagagtgtgc cattcatctc gattgcgccg cgggttggat catcaaaacc
    11641 tgtagtctgc ccctgacgat ttttccccat atctttagtg atggtgaacg cggtatacgg
    11701 cacgggtgca ttattcaagg cattaaaggt gcgtaccgtc atggtgatcg tgtcacccac
    11761 tttggcctgc gctctgctgc ctgcggaaac atcacgatcg gtggtcgcgg caatcttggc
    11821 gaccatctca ttgcccgaac aagagagata gttttgctta aagccggaat aggtgtcgat
    11881 gttgcccgtg gccaggttta ccgacgcgtg cgacgtctct tccaccgcac taagatacgc
    11941 tttttgcaac gttggccagc catattcggt gccgatggca ttggctggat gagcggcaaa
    12001 caaactgtgc aatgaacttt ggctcggaat atgacctaaa ccacactcag cctgcatact
    12061 gctattctga tcaaacagcg cccagtcttc gttattttcc cgcaccgatc ccagttcgtt
    12121 gtcggtttca tccgccagcc gtgggcgttt aaagatattg ttcgcttcaa taatgcccaa
    12181 catatggccc cacatccgcg ctttgtcggt atcggggctg gtgatcaccg taaaaataac
    12241 gtctttctca tccgaggcag taaaatcact gcgcattctc gcggtgatgt gagttttcac
    12301 ccccacgccg cctggctggg tcagcgtcag agtggcctct ccgttagcat cagtgatccc
    12361 ttcataggta tgggcgtcaa ctttgcgaac gttactgccg ccatcaattt gtaccggatg
    12421 cgtctcccac gcggcgtcag tttgattctt acggttaatg ccgtcatcca gatgcagcga
    12481 aaagtagtag taagccagcg gctgatcgtt gttcttgcta tcggtaatcg tcagacgtaa
    12541 agaagcctct tcccccacct ttactttcat cgcttttatc gtcgaatcat agctgcccgg
    12601 cgtcagtacc agcttcggtt caacatcggg cgcttcttta tcgacacagc tcacaataaa
    12661 cgtcgtatcg tccggttttt gcgcttcgct gcggttcgaa acatccgccg catggtgctg
    12721 gccgctcatc cccgccagtg atgaccagta gaaattaccc tgcatcggcc aaccagccac
    12781 atcctgcact gacgttggca ccaccgtcgc cagcgcaccg aactgacgca ttccgggcaa
    12841 aatgtcgcaa tgtttatccg cgccgctcca ggtaaaggtg gaccaggttt cattgtgatc
    12901 gttaaccgta ccgccttcat tatcgacttc cgccgccagt tttggtcgac taaaagtgta
    12961 atcacgggcc ttcagcgtat cgggcatatg accccacatt gtagcctgcg gaacatccgg
    13021 gctggtgagg acggtaaaga tcactgccgt ttcactggcc tgagcaatac ccgcgaggct
    13081 cgcgaccaac ggcgttttta caccagggcc gttggcctgg gtgaccgtaa ccgtggcaac
    13141 accattcgca tcggtagtac cacgatattc cgtcgcggtg gtggttaatt cggtcgtgcc
    13201 cagttttaca ggcgcagtat tgttcaccac accctggcgg ttttcagcat ctttgcgttt
    13261 gataacaaaa ggaatattcc ccacaacatt accgacgcaa tctttggtgg tgaccgttaa
    13321 cgtgatactg cccccaaccg tcattttcgc cgcacctgag gattcgtcat agcccgcagc
    13381 gctgacggaa ataaggtcac cctgcgcggg cgcaatcttc agcgtcactt tctgatcggc
    13441 gagcgtcaga acactttcat agggatagcc cgtcgaggtg agtaaatata acagcggcat
    13501 ggtctgctgg ggatcaggct gcgcccaggc gaccagcgaa cgcccggcaa gtccctgtgc
    13561 acaaaacgag cgatctgcga ctggctgtgg gtccagtaag gtgtccggcg tggcggcatc
    13621 cgcccaaact aaagatacag ccggtggcgt ttggttttcc caacgtgggg tggcaatgat
    13681 atcgccttct ctgtcttgcg ggttggtgac gagcaattta gagacgctaa catccgcgtt
    13741 gaattcactc ggtttagcgg taaacgctac ctcatgcgtc tttgtaggat ccagaaagac
    13801 actgccctgg tagacgggta tgctggcgct gtcggcggtg ggcacggtac cgttaaattc
    13861 gccgaccgta tctgaagagt cttgccatgc agattctgcc catgaccacc caggcagcat
    13921 tgcgcttatc agcaatgcgc ataacacaag gattttattc agggtcaggg acatgacaga
    13981 aactccaccg gttgttaaac ggacgcttgt cgcatcaaca tattttgctt aatccgggat
    14041 taagcaaaat atgcggaaac agcattccct tgcgggaatg ctgctgagta cgcgttattt
    14101 gtgtttgtaa ataatgtgaa gtttgtaacc ctgtaccccg tcgccgttgg tcgggatgga
    14161 aagtgcctct tttccatcgc gcgcagggcc gttaaggttg gttctggctt cgtcgttggt
    14221 cgcagggatg accaaattgt tattatcgaa gcttgagtta acgatgccac ccgaagtgcc
    14281 aagctgttgg ctgctgccag aaagtaccca acggaagtca taattggcgg tgacgtcagc
    14341 atcggtaccc gcatcaaact tgccattttt gttctcatcc gagtacagtt tggccacgta
    14401 ggttttgcca agttcgattg gcgtgctacc gttgataagg ttgatactgg taccatcaac
    14461 aaagatggcg actttcaggt tctggttaac caccggcccc ggtgcaacgt tatcgctgtc
    14521 gcttccgccg ccgctggcag tagaaacgtc ataaagatgc agcgcagtcc cgacgtttgg
    14581 cgtaccggtc tgcgtggttg gcgtaatttc aataccgata taacgaccac ggtcggcatc
    14641 ggtaagagtg taactggtca ccttcgttac cagctctttt ggatcacccc cctgcgcatc
    14701 cttgtagctc atccagcgga tagtatcgat agttttcgcg ttatcgccca atcccccaag
    14761 atccacgtcg ccttcggtat caccaatttc ccaacttacg gttaatttat cacccgagta
    14821 aagacgcttg tccgtttcac tgccttgcgg gcgagcgcct tttagatcag aagtcaacgt
    14881 tacacggtcg gcatctgtcg tagcacttcc cccttccgaa tagagccacg gaatggtccc
    14941 ctggaaaata ttggtttcac cgctgtcgac ccaggcgctg tcttcagcca gcgccacagg
    15001 tgccacacaa taacctgcca gtgccagcgc taatgcgatc cgggtcagac cgcgtctttt
    15061 atcctgcttc attgatgctc tccttaatga accttcagcg actggccgac tggccttacg
    15121 ccaatcttat tttttggtat acagcacgcg taaaccgtat ccctgcaagc catctcccgc
    15181 ttgtgcgcca taggtttgcg ccgcttcacg gttagtggcc ggaatcacaa tgtcctcatt
    15241 ggaagtgttc gcctgtgcac ccacttcacc attgatgctc ttgccggtaa attcccactt
    15301 gtagtcgtac tgcaccatct cttcgtcagt cagtttttcg acctgctgga agacaccgtc
    15361 tttattgttg tcgcgccaca aaacaaatcg ataagtgttg ttgagcggca acggttcttt
    15421 atctttacct atcaggttgc tgggtttagc cgcccccaca cgatagataa aagcatttag
    15481 cccaccaatt tctgccccga ggaaagtgac atccacatag ttactgtcat cgtagggcgc
    15541 ggcttttgct ttccagcgat aggtgccggg taaggtactg gagagggtga cagtgccctt
    15601 ttctttatct tgtttggcaa taaaaacgca ggggttatcg gtcgagcagg gcttgttggt
    15661 taacgtcact cgtttgtcct gcgcgtcgta aagctgaggc gtaaggttat cttccggcaa
    15721 cgcatcaccg ttgcggtcgg tgttatcgcc aaagctgtcg gttacatcga gatccatcac
    15781 tacaccatct acaccatcgg cctgtgcgct ggcttcgtgg ctgtggttgt tctcttctgg
    15841 cacgttcgcg atgttattaa caatcaactc tattttacgg tcctgctgaa cggtgatttc
    15901 caccacgcca gagttctgtc ggttgccctt ttcatcttcc agcgccagac gtactcgcca
    15961 ggtattgagt ttttcttgtt cggctttgtc agagctgtac tgccaggcgg gcagcaccag
    16021 gttccagtgg ttattgttac cctctgtcag ggtattctct ttgataaccg gcatcaccag
    16081 cgtgcgggca gcattgtttt tcgcctgcca ggaggcagca acgattttgt aatgatgatt
    16141 aatgagtgcg ttaatggtcc acttacattt ttccagaccg acggcttctt cgggcgtatc
    16201 tttaatcacg cactcgggat gttcattcgt gacgtcggct ttcagccaca aagtgacata
    16261 gaggggatct ttttctttgt attccagaac aataaagttg ttacgttcaa caaagtcatg
    16321 acggctaccc atgagcgagt gagccgcgct cactttgtca ggatcaagct ggtctttgag
    16381 cgggacgcca aactggtagt tcagcgttgc gttaacagaa agatcggtat tatcgccagt
    16441 gccagctttg aaatctgtac cgacaacaat gagcggaacg ggctggtatt tcacacccag
    16501 cgtgacagcg aagggatctt tctccagact atcggtacca aaaagcgcga cttcattccc
    16561 ataatattgt tcgaagacaa tttttccgcc aagctgcggg taagcgggta gccaggcttc
    16621 agcgcgaata tcccagccgc gcgcagggcg ttcttcataa aagtcgaaat cttcagagtc
    16681 tttccagtca gaaagtgggt gataatagtt gcctgagaat tttaaataat ccgtccaggc
    16741 ttcggcgcct aaacctaaac ggcgatgtcc acgggtaaag tcataatcat aaaatatatt
    16801 tccacccaat agatatttat caaaattata ccttacccca agccctatat ttccgatcgt
    16861 acgatcttct tttcgttgcg cagaaaattg gctgaaataa acagtggttt gattatcgta
    16921 ccagggaaca aaataatcga tggaactgcc atccagatcg ccgccctgac caatggagag
    16981 gttcgaacgg accttaccgt agggagagag tgtgtcttca atataagatt gtgcggttga
    17041 ggtgatttga ctttgcagat agcttctggc ctgagattcc agcgcctcag gggtcaagtt
    17101 ttcaaaccct tgcgtggcgg agttgatgac gtaatcggct ccgcgctctt tcagcgattt
    17161 acccttgttg gtttgttcat cctgttgtgc tgcctcgcta actaaatcag gtaacgattc
    17221 gttttgctta acatttatct ctggcgccgc gaaagcagca ccagagataa atgttagaat
    17281 aatacagcgt ttccatcgca gcatgcactt tccctgtgac aatgatacag tcaaaaataa
    17341 gctt
//
LOCUS       DADSZD010000095        16889 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.096, whole genome shotgun sequence.
ACCESSION   DADSZD010000095 DADSZD010000000
VERSION     DADSZD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.096"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(18..431)
                     /gene="hns"
                     /locus_tag="J1Z28_002845"
     CDS             complement(18..431)
                     /gene="hns"
                     /locus_tag="J1Z28_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBA7717664.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            1035..1652
                     /gene="tdk"
                     /locus_tag="J1Z28_002846"
     CDS             1035..1652
                     /gene="tdk"
                     /locus_tag="J1Z28_002846"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBA7717665.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            complement(1937..2984)
                     /locus_tag="J1Z28_002847"
                     /pseudo
     CDS             complement(1937..2984)
                     /locus_tag="J1Z28_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(3110..5785)
                     /gene="adhE"
                     /locus_tag="J1Z28_002848"
                     /gene_synonym="adhC"
     CDS             complement(3110..5785)
                     /gene="adhE"
                     /locus_tag="J1Z28_002848"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBA7717666.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            6262..6909
                     /gene="ychE"
                     /locus_tag="J1Z28_002849"
     CDS             6262..6909
                     /gene="ychE"
                     /locus_tag="J1Z28_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBA7717667.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            7647..9278
                     /gene="oppA"
                     /locus_tag="J1Z28_002850"
     CDS             7647..9278
                     /gene="oppA"
                     /locus_tag="J1Z28_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBA7717668.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9364..10284
                     /gene="oppB"
                     /locus_tag="J1Z28_002851"
     CDS             9364..10284
                     /gene="oppB"
                     /locus_tag="J1Z28_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBA7717669.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            10299..11207
                     /gene="oppC"
                     /locus_tag="J1Z28_002852"
     CDS             10299..11207
                     /gene="oppC"
                     /locus_tag="J1Z28_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBA7717670.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            11219..12232
                     /gene="oppD"
                     /locus_tag="J1Z28_002853"
     CDS             11219..12232
                     /gene="oppD"
                     /locus_tag="J1Z28_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBA7717671.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMDICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            12229..13233
                     /gene="oppF"
                     /locus_tag="J1Z28_002854"
     CDS             12229..13233
                     /gene="oppF"
                     /locus_tag="J1Z28_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBA7717672.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(13286..13615)
                     /locus_tag="J1Z28_002855"
     CDS             complement(13286..13615)
                     /locus_tag="J1Z28_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBA7717673.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWLEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(13650..15110)
                     /gene="cls"
                     /locus_tag="J1Z28_002856"
     CDS             complement(13650..15110)
                     /gene="cls"
                     /locus_tag="J1Z28_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBA7717674.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            15253..15426
                     /locus_tag="J1Z28_002857"
     CDS             15253..15426
                     /locus_tag="J1Z28_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBA7717675.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(15481..16734)
                     /gene="kch"
                     /locus_tag="J1Z28_002858"
     CDS             complement(15481..16734)
                     /gene="kch"
                     /locus_tag="J1Z28_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBA7717676.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         4306 a         3911 c         4189 g         4483 t
ORIGIN      
        1 gtgcaatcta caaaagatta ttgcttgatc aggaaatcgt cgagggattt accttgctca
       61 tccattgctt ttttgattac agctggagta cggccctggc cagtccaggt tttagtttcg
      121 ccgttttcgt caacgtagct atattttgcc ggacgctgag cacgcttagc tttggtgcca
      181 gatttaacgg cagcaaggct attcagcagt tcgttcgggt caataccgtc agcgatcagc
      241 atttcgcgat attgctgcag tttacgagtg cgctcttcaa cttcagcagc agccgcgctt
      301 tcttcttcgc gacgttcgtt aacaacaact tctaattttt ccagcatttc ttccagcgtt
      361 tcaagtgtac attctcttgc ctgcgcacga agagtacgga tgttgttcag aattttaagt
      421 gcttcgctca ttgtagtaat ctcaaactta tattggggtg gtttgttgag gtaataatag
      481 agccttaaat tcagttgtgc aatagccagg aatgtaagga attcaaaatt gttctttatt
      541 ttgtgccgcc aataaatatc ttttcataaa attagccaga aaagacgcgg catatagccc
      601 tatttgcacc gatgatttcg cagcacgtga ggttaaaact tcctgattca tgtcacattt
      661 tatggggaga taatcgtagg ctgacgacct ttcagtcttc tgtattagcc gtgtttacga
      721 gaattcccta ttaagcgaat gatgaaaagt agaacagtcg caataagagc agggacttag
      781 tattgcacta tctcctggag gtcaacagag ggctattaca tgcgcaacag gttaaagatt
      841 gtgaatagtt accggcagtc atttacccgc ttataacaag cgaggcagtt gtaatgatag
      901 ctcagaagga ttatgcaagg cttcgtaagg gagaacgcat ataccacttc tgtgcatact
      961 gttgagctga aaaactgacg aattatgata aactccagcc aactttattt catatcattg
     1021 agggcctgtg gctgatggca cagctatatt tctactattc cgcaatgaat gcgggtaagt
     1081 ctacagcatt gttgcaatct tcatacaatt accaggaacg cggcatgcgc actgtcgtat
     1141 atacggcaga aattgatgat cgctttggtg ccgggaaagt cagttcgcgt ataggtttgt
     1201 catcacctgc aaaattattt aaccaaaatt catcattatt tgatgagatt cgtgcggaac
     1261 atgaacagca ggcaattcat tgcgtactgg ttgatgaatg ccagttttta accagacaac
     1321 aagtatatga attatcggag gttgtcgatc aactcgatat acccgtactt tgttatggtt
     1381 tacgtaccga ttttcgaggt gaattattta ttggcagtca atacttactg gcatggtccg
     1441 acaaactggt tgaattaaaa accatctgtt tttgtggccg taaagcaagc atggtgctgc
     1501 gtcttgatca agcaggcaga ccttataacg aaggtgagca ggtggttatt ggtggtaatg
     1561 aacgctacgt ttctgtatgc cgtaaacact ataaagaagc gttagaagtc ggctcattaa
     1621 cggctattca ggaaaggcat cgccacgatt aataagaatt tctttactga cagggtgagc
     1681 agggcgcttt tatcctgtca gttcgtttta cgcacttctt ccgggctata cacccttctc
     1741 ggcagttttt taacgccgct atacgcctca cagagcgctt atacaccgac attgacttgt
     1801 aacctgtaaa gtacaatatc gctgtgtgta ggcgttatac atcgtcgcaa atatgatgaa
     1861 ggacaatgct gccggtttat ggaaaagttg ttttgggaaa acaaaaaacg gccccagaag
     1921 gggtaatgcc agtcagttaa gcaactgact ggctcttttt cggggctgtg gggtatttcc
     1981 agggcctctc ctttaccact ctcgggaagg ccctttccct tcttgtcggt aatttcactg
     2041 gttgtcccat acttgcaaga tcgcgcatca gctccggtat acgtcccggt gaagcgtatg
     2101 gcgtccgtca tcgacgtcag cagatggcag acttttcctt tgcgcgtcac ggtcagcagg
     2161 cgggctgtca cctcatttcc cagtcccggc cacttttttc gtgcctgcgg gctgatgttc
     2221 agcttcacca gatgatcgcc tttacccagt tttctgatct cttcatattg cgctcccttt
     2281 ctgagaggga tcatccagtg gcggtgttct cccgccaggc tccaggcatt taacagtcct
     2341 ggtgagtaat aacctttatc cattaacgtc agagtgttat cgccggtttg ttctataagt
     2401 tgctcagcaa gctcattttc gctgttcttc atcgtgccga aggctgcagt cgtcagcaga
     2461 tggctggtca gttccatctg gcagaccatt ttgacctgcg ggtagagcgc cgggttcccg
     2521 gcatgtgtct ggcgggggaa ggctgcatcg ttctctggtg tatccggtgt gcgccagaac
     2581 acaccatcga tggccagcag ggtcaggccg caccagtgcg gatgcggcgt ggcgttatgc
     2641 cagagctgcg ctgttttcgt gaacacgcgg cggacagcct caattcccag acgctggcgg
     2701 gtctgaataa cggcactggg ggcaacgaag gggcgattgc ccggcagcat gatgtccagg
     2761 cgattcacaa tctggtgaag aggttcttta cgctcaagcg ccatgccaac aatacaccag
     2821 accatcattt cgaggggaag acggcgcttg cgtagcgtta cagtacctga ttcggcaagg
     2881 caacgagaga tgagtttggg ggcgaggtaa tcccccagag aagtcagtgg gttacgcaga
     2941 gaatcgtaac gggataccaa atcaagggcc tgcccaatga gcataaaaaa atccggaaac
     3001 gagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc cttaactgac
     3061 tggcattacc ccagaagggg ccgtttatgt tgccagacag cgctactgat taagcggatt
     3121 ttttcgcttt tttctcagct ttagccggag cggcttcttt cttcgctgca gtttcgcctt
     3181 ctacataatc acgaccgtag taagtatcca gcagaatctg tttcagctcg gagatcagcg
     3241 ggtaacgcgg gttagcgccg gtgcactggt catcgaatgc atcttcagac agtttatcca
     3301 cgttcgccag gaagtctgct tcctgaacgc cagcttcacg gatagatttc ggaataccca
     3361 gttcagcttt cagcgtttcc agccatgcca gcagtttctc gatcttagct gcagtacggt
     3421 cgcccggtgc gctcagaccc aggtggtcgg caatttcagc ataacgacgg cgagcctgcg
     3481 gacggtcata ctggctgaat gcagtctgct tggtcgggtt gtcattcgcg ttgtagcgaa
     3541 taacgttaca aatcagcagg gcgtttgcca gaccgtgcgg aatatggaac tgggaaccca
     3601 gtttgtgcgc cattgagtga catacaccca ggaaggcgtt cgcaaacgcg atacccgcga
     3661 tagtcgctgc actgtgaaca cgttcacgcg ctaccggatt tttagaccct tcgtggtagg
     3721 acgctggcag atattctttc agtagtttca gtgcctgcag agcctgacca tcagagaact
     3781 cagatgccag tacagaaaca taagcttcca tggcgtgagt tactgcgtcc agaccaccga
     3841 aagcacacag ggacttcggc atgtccataa caaggttggc gtcgacaatc gccatatccg
     3901 gggtcagtgc atagtctgcc agcggatatt tctgaccagt agtgtcgtca gttacaaccg
     3961 caaacggagt gacttcagaa cctgtaccag aagtggtggt gacagcgatc attttcgctt
     4021 tcacacccat tttcgggaac ttgtagatac gtttacggat atccataaag cgcagcgcca
     4081 gttcttcgaa gtgagtttcc ggatgttcgt acataaccca catgatcttc gcagcgtcca
     4141 tcggggaacc accacccagc gcgataatca cgtctggttt gaaggagttt gccagttctg
     4201 cacctttacg aacgatgctc agggtcgggt cagcttctac ttcgaagaag acttcagttt
     4261 caacgcctgc tgctttcaat acggaagtga tctgatcagc atagccattg ttgaacagga
     4321 agcggtcagt cacgatgagc gcacgtttgt ggccatcagt aatcacttca tccagcgcga
     4381 ttggcaggga gccacggcgg aagtagatag atttcggaag tttgtgccac aacatgtttt
     4441 cagctcgctt agcaacggtt ttcttgttga tcaggtgttt cggaccaacg ttttcagaga
     4501 tggagttacc accccaggaa ccacaaccca gagtcaggga aggtgcgagt ttgaagttat
     4561 acaggtcacc gataccaccc tgagaagccg gggtgttaat caggatacga gccgttttca
     4621 ttttctgacc gaagtaagaa acgcgagccg gttggttatc ctggtcagtg tacaggcaag
     4681 aggtatgacc gataccgccc atagcaacca gtttctctgc tttttctacc gcgtcttcga
     4741 aatctttagc acggtacatt gccagagtcg gggacagttt ttcatgtgcg aacggttcgc
     4801 tttcatcaac aacggtcact tcaccgatca gaatcttggt gttttctggt acagagaagc
     4861 ctgccagttc agcaatttta taggctggct gaccaacgat agccgcgttc agcgcaccgt
     4921 ttttcaggat aacgtcctga acagctttca gctctttacc ctgcaacaga tagccgccgt
     4981 gggttgcgaa acgttcacgt actgcgtcat aaacagagtc aacaacaaca acagactgtt
     5041 cagaagcaca gattacaccg ttgtcgaagg ttttggacat cagtacagat gcaactgcac
     5101 gtttgatatc agcagtttca tcgataacaa ccggagtgtt gcccgcgcct acgccgatag
     5161 ctggtttacc ggagctgtat gcggctttaa ccatgcccgg accaccagtc gcgaggatca
     5221 ggttgatgtc tgggtggtgc atcagtgcgt tagacagctc aacagaaggt tgatcgatcc
     5281 agccgatcag atctttcgga gcaccggcag cgatagcagc ctgtagaacg atatcagccg
     5341 ctttgttggt tgcatctttt gcacgcgggt gcggggagaa gataatggcg ttacgggtct
     5401 tcaggctgat cagcgatttg aagatagcag ttgaagtcgg gttagtggtc ggaacgatac
     5461 cgcaaataat accgatgggt tcagcgatag tgatggtacc aaaagtgtcg tcttcagaca
     5521 gaacaccaca ggttttttca tctttatagg cgttgtaaat atattcagaa gcaaagtggt
     5581 ttttgatcac tttatcttcg acgataccca tgccggattc ggcaacggcc attttcgcga
     5641 gtgggattcg agcatctgca gcagccagag cggcggcgcg gaagattttg tctacttgct
     5701 cttgagtgaa actggcatat tcacgctggg ctttttttac acgctctacg agtgcgttaa
     5761 gttcagcgac attagtaaca gccataatgc tctcctgata atgttaaact tttttagtaa
     5821 atcatctgct cgaatacgag agtatagtca gtgcggtgat gatttgctta accgatggaa
     5881 atcaaaagct tactcgcgct cacactcact gtgatttact aaaagagttt aaacattaga
     5941 gttattatct ctaatgcgtc acttccaggt ggcgtaagca agattactca cttctgggta
     6001 ctgattacgt gatccaaatc aaaattttgc aaagctgaca cctttcagca tggcttttcg
     6061 ccattatagc taacagttaa taaattgtag tatgatttgt tggctacatt agcatgtttt
     6121 gcacaactag gtaacaataa cgaatgatag caattttaag tagttaggag gtgaaaaatg
     6181 ctgtcaaaag gcgtattgtc agcgcgtctt ttcaacctta tttatggcta acattatccg
     6241 gcttttgctt cggagctaac cgtgattcag accctttttg attttcccgt ttacttcaaa
     6301 tttttcatcg ggttatttgc gctggtcaac ccggtaggga ttattcccgt ctttatcagc
     6361 atgaccagtt atcagacagc ggcagcgcga aacaaaacta accttacagc caacctgtct
     6421 gtggccatta tcttgtggat ctcgcttttt ctcggcgaca cgattctaca actttttggt
     6481 atatcaattg attcattccg tatcgccggt ggcatcctgg tggtgacaat agcgatgtcg
     6541 atgatcagcg gcaagcttgg tgaggataaa cagaacaagc aagaaaaatc agaaaccgcg
     6601 gtacgtgaaa gcattggtgt ggtgccattg gcgttgccgt tgatggcggg gccaggggcg
     6661 atcagttcta ccatcgtctg gggtacgcgt tatcacagca ttagctatct gtttggtttc
     6721 tttgtggcta ttgcattgtt cgctttatgt tgctggggat tgttccgcat ggcaccgtgg
     6781 ctggtacgag ttttacgcca gaccggcatc aacgtgatta cgcgtattat ggggctattg
     6841 ctgatggcgt tggggattga atttatcgtt actggtatta aggggatttt ccccggcctg
     6901 cttaattaat tcctttcaaa tgaaacggag ctgccatgct ccgtttactt cgtcattatt
     6961 tttactttgt tcctgcgcag ttatcaaaag caaaaggaat aggtaaaaat attcttctca
     7021 aattacagtt agttataggg atttccttaa ctgcttctcc tcaccatcat gttattttcg
     7081 ccacatcata atcctgggct tgctgaagaa taattgaaat gatattagta attccactgc
     7141 ctttggtaga ggaaagtgct aaataataat caattgttaa attattgtgc atttcactac
     7201 tggaactgta atcagaaaag agagacatgc ttaaccaacc tctatttgat tgaattgaaa
     7261 gatgtttgtt aaggcatgga tgcaagctat agattctgat acggtcaata aaagagaatt
     7321 gcttaacaat tttgcaaaat gtattggcga gtaagaaccg catttggtac tttccgggca
     7381 accgccaggc gattctttat tggtaatgag aataattaac aattaaagat cgtcgcgaaa
     7441 gaataatgtg tctcgacagg ggagacacag tacgaatcga cataaggtga tcgtctgaat
     7501 caccagaata aataaagtcg gtgatagtaa tacgtaacga taaagtaacc tgacagcaga
     7561 aagtctccga gcctgtgcag ggtcccaatc cgggattaca catgctggtt aataccagta
     7621 attataatga gggagtccaa aaaacaatga ccaacatcac caagagaagt ttagtagcag
     7681 ctggcgtttt ggctgcgctt atggcaggga atgtcgcgct ggcagctgat gtacccgcag
     7741 gtgtcacact ggcggaaaaa caaacgctgg tacgtaacaa tggttcagaa gttcagtcat
     7801 tagatccgca caaaattgaa ggtgttccgg agtctaatat cagccgagac ctgtttgaag
     7861 gcttactggt cagcgatctt gacggtcatc cggcacctgg cgtcgctgaa tcatgggata
     7921 ataaagacgc gaaagtctgg accttccatt tgcgtaaaga tgcgaaatgg tctgatggca
     7981 cgccagttac agcacaagac tttgtgtata gctggcaacg ttctgttgat ccaaacactg
     8041 cttctccgta tgccagttat ctgcaatatg ggcatatcgc cggtattgat gaaattcttg
     8101 aagggaaaaa accgattacc gatctcggcg tgaaagctat tgatgatcac acattagaag
     8161 tcaccttaag tgaacctgtt ccgtacttct ataaattact tgttcaccca tcaacttcac
     8221 cggtgccaaa agccgctatc gagaaattcg gcgaaaaatg gacccagcct ggtaatatcg
     8281 tcaccaacgg tgcctatacc ttaaaagatt gggtcgtaaa cgaacgaatc gttcttgaac
     8341 gcagcccgac ctactggaac aacgcgaaaa ccgttattaa ccaggtaacc tatttgccta
     8401 ttgcttctga agttaccgat gtcaaccgct accgtagtgg tgaaatcgac atgacttata
     8461 acaacatgcc gatcgaattg ttccagaagc tgaaaaaaga gatcccggac gaagttcacg
     8521 ttgatccata cctgtgcact tactattacg aaattaacaa ccagaaaccg ccatttaatg
     8581 acgtgcgtgt gcgtaccgca ctgaaattgg gtatggaccg cgatatcatt gttaataaag
     8641 tgaaagcgca gggcgacatg cccgcctatg gttacactcc accatatact gatggcgcaa
     8701 aattgactca gccggaatgg tttggctgga gccaggaaaa acgtaacgaa gaagcgaaaa
     8761 aactgctggc tgaagcgggt tataccgcag acaaaccgtt gaccatcaac ctgttgtata
     8821 acacctccga tctgcataaa aagctggcga ttgctgcctc ttcattgtgg aagaaaaaca
     8881 ttggtgtaaa cgtcaaactg gttaaccagg agtggaaaac gttcctcgac acccgtcacc
     8941 agggcacttt tgatgtggcc cgtgcaggct ggtgtgcaga ctacaacgaa ccaacttcct
     9001 tcctgaacac catgctgtcg aacagctcaa tgaataccgc gcattataag agcccggcct
     9061 ttgacagcat tatggcggaa acgctgaaag tgactgacga ggcgcagcgt acagctctgt
     9121 acactaaagc agaacaacag ctggataagg attcggccat tgttcctgtt tattactacg
     9181 tgaatgcgcg tttggtgaaa ccgtgggttg gtggctatac cggcaaagat ccgctggata
     9241 atacctacac ccggaatatg tacattgtga agcactaatg gcaatacgtg gggcaggagt
     9301 gtcctgctcc acggtgtctg atttttatcg cattacagaa ggcacaggcc agaaggtagg
     9361 gcaatgttaa aatttattct acgtcgctgt ctggaagcga ttccgacgct atttattctt
     9421 attactattt cgttctttat gatgcgcctc gctccgggca gtccttttac cggcgaacgt
     9481 actttaccac cggaagtgat ggccaatatc gaagcgaaat atcatcttaa tgatccgatc
     9541 atgacacagt atttcagcta cctgaaacaa ctggcgcatg gcgatttcgg tccatcgttt
     9601 aaatataaag attattcggt caatgacctg gttgcatcca gttttcccgt ttctgccaaa
     9661 ctgggagccg cagcattttt tcttgcggta atactgggtg ttagtgctgg cgttattgcc
     9721 gcattaaagc aaaacaccaa atgggactat accgtgatgg ggctggcaat gaccggggtt
     9781 gttatcccca gttttgtggt tgcgccatta ttagtcatga tatttgcgat cattttgcat
     9841 tggctgccgg gcggtggctg gaatggtgga gcgcttaaat tcatgatatt gccaatggtg
     9901 gcgttatcac tcgcctatat cgccagtatt gcgcgtatta cccgtggctc tatgattgag
     9961 gttttacact ccaactttat tcgtactgcc cgggcgaaag ggttacctat gcggcggatc
    10021 attttacgcc acgcattaaa acctgctctg ttacccgtgc tctcctatat ggggcccgca
    10081 tttgtcggca ttattaccgg ttcgatggtc atcgaaacca tttatggttt accggggatt
    10141 gggcaattgt tcgttaatgg cgcattgaac cgtgactatt ccttagtgtt aagtctgacc
    10201 attctggttg gcgcgttaac cattttgttt aatgccattg tcgatgtgct atatgcggtt
    10261 atcgacccga aaatccgtta ctgatactgg agctcgcgat gatgttaagt aagaaaaaca
    10321 gcgagacgct ggaaaatttc agtgaaaagc tggaggtcga agggcgcagc ttgtggcagg
    10381 acgcacgtcg acgttttatg cataaccgtg cggcggttgc cagtctgata gtgctggtgc
    10441 tgatcgcgtt atttgtaatc ctggcaccga tgctttcgca gtttgcctat gacgatactg
    10501 actgggcgat gatgtccagc gccccggata tggagtccgg tcactacttt ggtactgact
    10561 catccggtcg cgacctgctt gtgcgcgttg cgattggcgg gcgtatctca ctcatggtcg
    10621 gtgttgctgc ggcactggtg gcagtggtcg tggggacact ttatggttcg ctttccggtt
    10681 atctgggcgg taaagtggat tcggtaatga tgcgtctgct ggaaatcctc aactccttcc
    10741 cattcatgtt cttcgtcatt ttgctggtga cctttttcgg tcaaaacatc ctgctgattt
    10801 tcgtggcgat tggcatggtt tcctggctgg atatggctcg tattgtgcgt gggcaaaccc
    10861 tgagtctgaa gcgcaaagag tttattgagg cggcacaagt tggcggtgta tcgacgccgg
    10921 gcattgttat tcgccacatt gtgccgaacg tactcggtgt ggtggtggtc tatgcgtcgc
    10981 ttctggtgcc cagcatgatc ctctttgaat ctttccttag cttcctggga ttaggtacgc
    11041 aagagccgtt aagcagctgg ggtgcattgc tgagtgatgg cgcgaactcg atggaagtct
    11101 ctccatggtt actgttgttc cctgcgggat tcctcgtggt aacgctgttt tgtttcaact
    11161 ttatcggcga tggcttgcgt gatgccctcg acccgaaaga tcgttaagga gtgcagccat
    11221 gagcgtaatt gaaactgcaa ctgtgccgct cgcacaacaa caggctgacg cactgctgaa
    11281 cgtgaaagat ttgcgtgtca cctttagtac cccggatggc gacgtcacgg cggtcaatga
    11341 tttgaatttt tcgctacgtg ccggagaaac gctgggcatc gtaggtgagt ctggttcggg
    11401 caaatcgcaa accgcatttg cgttgatggg cctgctggct gccaacggac gtattggcgg
    11461 atcggcaacc ttcaatgggc gtgaaatcct caatttgcca gagcgtgaac tcaataaact
    11521 gcgagctgaa caaatctcaa tgatttttca ggacccaatg acttcgttga atccctatat
    11581 gcgcgtcggt gagcagttga tggaagtgct gatgctgcat aagaacatga gcaaagctga
    11641 ggcgtttgaa gaatcggtgc ggatgctcga tgcggtaaaa atgccggaag cgcgtaaacg
    11701 catgaaaatg tacccgcacg aattttctgg cggcatgcgt cagcgagtca tgattgcgat
    11761 ggcattgcta tgtcgaccta agctgctgat tgcggatgaa cccactactg cgctggacgt
    11821 caccgtacag gcgcagatca tgacgctatt gaatgaactg aagcgggaat ttaataccgc
    11881 catcattatg attacccacg atcttggcgt ggtagcgggg atctgcgaca aagtgctggt
    11941 aatgtacgcc gggcgcacga tggaatatgg caacgcgcgc gatgtctttt atcaacccgt
    12001 acatccttat tctatcggtt tgctcaacgc ggtgccgcgt ctcgatgcgg aaggtgaaac
    12061 aatgttgact attccgggta atccgccaaa cctgctgcga ttaccgaaag gttgcccgtt
    12121 ccagccacgt tgtccgcatg cgatggacat ttgtagtagc gcaccgccgc tggaagagtt
    12181 tacgcctggc cgtctgcgtg cttgctttaa accagtggag gaactgttat gaatgctgta
    12241 accgaaggaa gaaaagtcct cctcgaaatc gccgatctga aagtgcactt tgaaatcaaa
    12301 gatggcaaac agtggttctg gcaaccgccg aaaacgctca aagccgtcga tggtgtaact
    12361 cttcgcctgt atgaagggga aacattaggt gtggtagggg aatcgggatg cgggaagtcc
    12421 acctttgctc gcgccatcat tggcttggtt aaagcgaccg acggtcatgt ggcctggtta
    12481 ggtaaagagt tgctgggcat gaagcccgat gaatggcgtg ccgtacgcag tgatattcag
    12541 atgattttcc aggatccgtt ggcatcactg aacccgcgta tgaccatcgg cgagatcatc
    12601 gctgaaccac tgcgtactta tcatccgaaa atgtcacgcc aggaagttcg cgagcgcgtg
    12661 aaggcgatga tgctgaaagt cggtttgttg cctaacctga ttaaccgcta tccgcatgag
    12721 ttctccggtg ggcagtgcca gcgtatcggg attgctcgtg ctcttattct tgaaccgaag
    12781 ctgattatct gtgatgagcc ggtgtcggcg ctggacgtgt caattcaggc gcaggtggtc
    12841 aacctgctcc agcagttgca acgtgagatg ggattgtcat taatttttat cgctcacgac
    12901 ctggccgtgg taaaacacat ttccgatcgt gtgctggtga tgtatctcgg ccatgcggta
    12961 gaactgggga cctatgatga ggtctaccac aatccgctac atccttacac cagggcattg
    13021 atgtcggcag tccccatacc tgatccggat ctggagaaga acaaaaccat ccagttactg
    13081 gaaggggaat taccgtcgcc gatcaacccg ccttccggtt gtgttttccg tacccgttgt
    13141 ccgattgcag gtccggagtg cgccaaaaca cgtcctgtgc tggaagggag tttcagacac
    13201 gccgtttctt gcctgaaagt cgatccgctt taaaagcaat aagggctgac agttgtcagc
    13261 cctttttcac gccaaaagcg attatttatt cccgccagat gatatggcaa agtttgtgat
    13321 ctttttcgcg gcataacaga atgcgcgcga aaacatcgtt gatttcacca tcttcactgt
    13381 ccgccagacc aatcaccact tcggcaaaaa agtccgggtt caaatcgaaa tcaacatgtt
    13441 ccagccagtc ttccgccgga tcgaataact ccgcgccgcc gcgctcttca aactgaagat
    13501 tgaacagcag aacatcagct ggatcgagat tgtcggcagc cagttcgaga aaaatatcgt
    13561 aggcctgctc aagcgtttca tcttcagtca ggcgattgtt tagatccata tccatagtca
    13621 ctacctgttt aacctctgtt ggcgacgttt tacagcaacg ggctgaagaa gtaaaacagt
    13681 cgctcggcga cacgttgcca taatggacgt tttagccata aacgggcatc gagcagacga
    13741 gaacgcgaaa tataatcgtc ctgaacggcg gcgaggtctg caccaaaacc tttatcgtcg
    13801 attgccaggg taatctcgaa atttagccac agactacgca tatcaaggtt aacggtgcca
    13861 accagactta gttcaccatc gaccagcacg ctcttggtat gcagtaaccc gccttcaaac
    13921 tgataaattt taaccccagc agccagcagt tctgtaaaga atgcgcgact ggcccagccg
    13981 accagcatcg agtcattttt tcgcggaagg ataatactga catccacccc gcgctgcgcc
    14041 gccgtgcaaa tcgcatgaag taaatcatcg cttggcacaa aatagggcgt tgtcatgatc
    14101 aaatattcac gagccgaata agcagcagtc aataatgcct ggtgaatgag atcttccgga
    14161 aagccggggc cagaagcaat tgtgtgaata gtgtgaccgc tggcctgctc aaacggcata
    14221 atattgacat ctggtggcgg cggcagaata cgtttcccgg tttcaatctc ccagtcgcag
    14281 gaataaataa tccccatcgc ggtggcgatg gggccttcca tacgcgccat cagatcaatc
    14341 cattgcccta cgcccgcatc ttgtttgaag tagcgagggt cgaccatatt catgctgccg
    14401 gtgtacgcga tgtaattatc aatcatgatc atcttgcgat gttggcgcag gtccatacgg
    14461 cgtaaaaaca cacgcatcag attgaccttt aaggcttcga caacttcaat accggcatta
    14521 cgcattagct cgggccacgg gctgcggaaa aaagccacac tcccggcgga gtcgagcatc
    14581 aatcggcaat gaataccgcg ccgtgcagcg gccattaatg attcagccac ctggtccgcc
    14641 atgccgccgg gctgccagat ataaaacacc atctcaatat tatggcgcgc gagctggatg
    14701 tcgcggatta acgcctgcat cacatcatct gactcggtca tcagttgtag ctgattccct
    14761 ttgaccccag cgatcccctg acgacgctcg caaagcttga ataatggcgc agcgacactg
    14821 ctattttctt cggcgaagat atgcttacag gctttaaggt cgttaagcca ttttgcggtg
    14881 gaaggccaca tcgctctggc gcgctcagcg cggcgtttgc ctaaatggag ctcgccaacg
    14941 gcaagatagg caataattcc gactaacggc agaatgtaaa taatcaacag ccaggccatc
    15001 gcggagggaa ctgcgcgtcg tttcattaga atgcgtaaag ttacgcctgc aatgagcaac
    15061 cagtatccca gaatggccaa ccaactcacc aacgtataaa cggttgtcat agattagaaa
    15121 tccttttgaa agcgcattgt tatgagttta cgcatatgga ttcatctggc aaataaaaac
    15181 gcgggaaaag cactggtcag cgtcaggggc gggggtataa tgaccattct gttgttgcat
    15241 agagtagtta acatgaagcg gagtagaacg gaagtggggc gctggcgcat gcagcgtcag
    15301 gctagccgac gtaaatcgcg ttggcttgag gggcagtcgc gccgaaatat gcgtatccac
    15361 agcatcagga agtgcattct aaacaaacag cgtaactcgt tattgtttgc gatctacaat
    15421 atctaaatgt actgggcacc attgcggtgc ccaataagtt tactcttccg tctttaaact
    15481 ctatttttgc gccgattctt tactgtcagc tttcgtttct tgttcatcgt tatcgctgaa
    15541 aatgccatga ccggagttca acaacattga aacgagcata tcattattaa tctcttcacc
    15601 atttaaaact cgcgccagaa tttcgctgcc aaacagttgt ggtgagagaa tgatatccgg
    15661 atgtaccatc ttaatcttat tcaggttttt gctatcactg acggcgagaa ctgttttgac
    15721 atcactgctc atatcttttg ccgagagtac aacaaacgcg ttatctgcat cgttatcact
    15781 cagcgccaga atggcccggc aacgatcgat tcccgctttc tttaacactg aactgtcatt
    15841 actgtcaccg gggataacat cggcgttatc gcctaagcgt tgctcaagtt gcttgatatc
    15901 atcttcaggc aagttgctga taaccgttac gttttgtccg cgttgattca gttgcagaat
    15961 cgtattgatg gcgagaatcg aatgtccgca aacaataaaa tgatctttac gatgcattgt
    16021 atgattgttt ccttttacaa gtttgttgaa tcccccgcgg ataagcggac caaaaattga
    16081 ggtcatagat gtggcaaaaa cggtaatgcc ggaaataatg accgaaatag tgaacaatcg
    16141 tgctgattca gaaacaggga caatatcgcc atagccgacg gttgacatgg tttctatcga
    16201 aaaatagaac gcggtcatca aactttctat tcgcggatta aaaccttcgc ttaaataaag
    16261 cgcaccgtaa gtggagtaaa acagtaacgt cgtgaaacta ataaatgcga aaattgtccc
    16321 ggctgctgcg ctactgtggg agaagtcttt gcgcagtatc agcaaaaaca ccagcgtaaa
    16381 aatgcaaaat ccaatactaa atttcagcca gggataaaaa tgcagagtgt aaattagcgc
    16441 tatcaacaac aaaatgatac tgattgccca tgcgagcttt gcccggaaca gtaagccaat
    16501 ggagttcagg acaagaaata cgccgagcat aaagagtggg gcgtttgcca gcaaggagag
    16561 atccagctct gaaaatgctt tgatatggaa aatatcaagc aaattgaccg acataccata
    16621 gattgtttta aaaataagca atccatttaa aaagacggcc agcgcgagaa tgtcgtgccg
    16681 tagggtcacc cataactttg ttgctgtttg tttgaatgta gtccagtgac tcactgatac
    16741 ctctgtaacc acaattttgt ttaattatct caacgtgcac attttctgaa catacatgca
    16801 gcgcgtgaat gtgttaaaca atagtagatg actttatccg cagtaatcaa aggttttgag
    16861 tgagcaactc tgcgtcagga aagggcatt
//
LOCUS       DADSZD010000096        16755 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.097, whole genome shotgun sequence.
ACCESSION   DADSZD010000096 DADSZD010000000
VERSION     DADSZD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.097"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(609..985)
                     /gene="rnpB"
                     /locus_tag="J1Z28_002859"
     ncRNA           complement(609..985)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="J1Z28_002859"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1018..2163)
                     /gene="garK"
                     /locus_tag="J1Z28_002860"
     CDS             complement(1018..2163)
                     /gene="garK"
                     /locus_tag="J1Z28_002860"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBA7717677.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2260..3150)
                     /gene="garR"
                     /locus_tag="J1Z28_002861"
     CDS             complement(2260..3150)
                     /gene="garR"
                     /locus_tag="J1Z28_002861"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA7717678.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETAATAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3180..3950)
                     /gene="garL"
                     /locus_tag="J1Z28_002862"
     CDS             complement(3180..3950)
                     /gene="garL"
                     /locus_tag="J1Z28_002862"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBA7717679.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3966..5300)
                     /locus_tag="J1Z28_002863"
     CDS             complement(3966..5300)
                     /locus_tag="J1Z28_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7717680.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5675..7246
                     /gene="garD"
                     /locus_tag="J1Z28_002864"
     CDS             5675..7246
                     /gene="garD"
                     /locus_tag="J1Z28_002864"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBA7717681.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7395..7730
                     /gene="prlF"
                     /locus_tag="J1Z28_002865"
     CDS             7395..7730
                     /gene="prlF"
                     /locus_tag="J1Z28_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBA7717682.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7730..8194
                     /locus_tag="J1Z28_002866"
     CDS             7730..8194
                     /locus_tag="J1Z28_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YhaV family
                     toxin"
                     /protein_id="HBA7717683.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(8249..9058)
                     /gene="agaR"
                     /locus_tag="J1Z28_002867"
     CDS             complement(8249..9058)
                     /gene="agaR"
                     /locus_tag="J1Z28_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBA7717684.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9307..10587
                     /gene="kbaZ"
                     /locus_tag="J1Z28_002868"
     CDS             9307..10587
                     /gene="kbaZ"
                     /locus_tag="J1Z28_002868"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBA7717685.1"
                     /translation="MKHLTEMVRQHKEGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGAADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLSAIWP
                     RIIALVVQPGVEFDHTNVIDYQPVKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            10610..11083
                     /gene="agaV"
                     /locus_tag="J1Z28_002869"
     CDS             10610..11083
                     /gene="agaV"
                     /locus_tag="J1Z28_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBA7717686.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            11094..11873
                     /locus_tag="J1Z28_002870"
     CDS             11094..11873
                     /locus_tag="J1Z28_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBA7717687.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11863..12741
                     /locus_tag="J1Z28_002871"
     CDS             11863..12741
                     /locus_tag="J1Z28_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBA7717688.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12759..13193
                     /locus_tag="J1Z28_002872"
     CDS             12759..13193
                     /locus_tag="J1Z28_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7717689.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            13190..14323
                     /gene="nagA"
                     /locus_tag="J1Z28_002873"
     CDS             13190..14323
                     /gene="nagA"
                     /locus_tag="J1Z28_002873"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA7717690.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDV
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMAGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14673..15827
                     /locus_tag="J1Z28_002874"
     CDS             14673..15827
                     /locus_tag="J1Z28_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HBA7717691.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVKLANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVITAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15840..16700
                     /gene="kbaY"
                     /locus_tag="J1Z28_002875"
     CDS             15840..16700
                     /gene="kbaY"
                     /locus_tag="J1Z28_002875"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HBA7717692.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         4065 a         4422 c         4272 g         3996 t
ORIGIN      
        1 ctttttctct tctgacagga cgtcattttg tgaatgcaat cgttttccat aaattcttct
       61 cccctcatag gtaacgaata gcattttgtg ttgaggatca caaaacgaat aattgctggt
      121 cgccgcgata aggtcagacg aagtcaacaa ggaggatttt cgctgtgctt tacatcagcg
      181 taggccatag aatgttgctt cgttgactgt aatgccgggt aaatgagcat tttttgatag
      241 tgcgaaagaa ccctgcgagg aaaataagca tctattattg tttgtcatga tgacaaggca
      301 ggcatcaatg aaaggcttct caattgcaca tatttttcac ccttcaatgc acgcagtggt
      361 taacaatcac aatagaaata ttgattattg gacggtaaaa atttaggtta taacgcccaa
      421 atgattcaac gatcggacaa tacctgcgaa cttacaatta atgagagaaa tatatgtata
      481 attacgacct tttaaatgca atatagactt aataacttct tacaaagtct gttattaata
      541 ccgtagatat tcatcttacc ctgggcacca aaagcaaaaa cccgccgaag cgggttttta
      601 cgtaaatcag gtgaaactga ccgataagcc gggttctgtc gtggacagtc attcatctag
      661 gccagcaatc gctcactggc tcaagcagcc tacccgggtt cagtacgggc cgtaccttat
      721 gaacccctat ttggccttgc tccgggtgga gtttaccgtg ccacggactg ttaccagccg
      781 cgcggtgcgc tcttaccgca ccctttcacc cttacctgat cccgcttacg cgggccatcg
      841 gcggtttgct ctctgttgca ctggtcgtgg gtttcccccc caggcgttac ctggcaccct
      901 gccctatgga gcccggactt tcctcccctc cgcccgtctc ccccgaagag gacgacgacg
      961 aagcggcgac tgtctggtca gcttcggcgc gcagtataga gggtttgcgc gcccttgtca
     1021 ccccgcgttg cgcattccaa tcgccagtgt cgcggcgata ttacgtgaag cacggcagat
     1081 attgtcataa gctccgcgga atgcttcgtc taacgtacca atgctggtca gtacgctgaa
     1141 gaccgcatca atgccatgct gatgtacaac gccaacatca ttggtcaggc tacccgcaat
     1201 gccaatcacc ggtttatggt acttcttcgc cacgtttgcg acaccaatcg gcaccttgcc
     1261 gtgaatactc tggctatcaa tacgcccttc accagtgatc accagcgtac aatcgtgaat
     1321 atgttcctcc agattcagcg ccgtagtgac gatttcaata ccacttttca gttctgcacc
     1381 aagaaacgcc atcagcgccg cgcccatacc gcccgcagct cctgcgccgg ggacatcttt
     1441 cacatcaaca tgcagcgctt ttttaatgac atcggcatag tgagagaggt tattatccag
     1501 ctcgacaatc atcgcctcac tggctccctt ttgtgggcca aagatgcgcg aagcaccact
     1561 gtcgccgact aacgggttgg tgacatcaca agcgacgcgg atgacacaat cttttaagcg
     1621 cggatcgaga ccggaaatat caatatcatt cagagtatta agactaccgc cgccaaaacc
     1681 aatttcattg ccgttggcat cgcataattt cgcccccagc gcctgtacca tgcctgcgcc
     1741 gccgtcgttg gtggcgctgc cgccaatgcc gataataatg tttgtcgcac cgctctccag
     1801 cgcctgcagg attaactcac cagtaccgcg tgaagtggtc acgagtggat cgcgtttttc
     1861 cgcaggtacc agttccagcc cactggccgc cgccatttcg ataaacgcgg ttttgccatc
     1921 gccggagatc ccccaactgg cattcacttt ctcgcccagc ggccctgtaa cccaggcgtg
     1981 acgttcagcc ccctgggtgg ctgcaatcat cgcttccacc gttccttcac caccgtcggc
     2041 aaccggaaca gaaacatact gtgcatcagg aaaaatttcc cgaaatcctt tttctatcgc
     2101 ctgcgcaacc tcgctggcag ataaactttc tttataagag tctggggcga ttacgatttt
     2161 catacctatg cctgttacca catgacgccg gagggcgttt ctcttattcg gcctggattc
     2221 caggcccgga ttgcaatacg ccatccgggc acgacgtcat tagcgagtaa cttcgacttt
     2281 cgccagtttt tcgtagtagc acgccagggc gctatgatcc gccgttccta aaccatctgc
     2341 acgcagtgcc tgcatcatct ccataaccgc agctgtgagc ggcagttgcg cgccgacgcc
     2401 gtgagaagta tccagcgcat tcgccagatc cttaatatgc agatcaatac ggaagcccgg
     2461 tttgaagttg cggtccatca ccatcggcgc tttggcatcc agcacggtac tgcccgccag
     2521 cccaccgcga attgcctgat acaccagatc cgggttaacg cccgctttgg ttgccagcgt
     2581 taacgcttct gacatcgcag caatattcag cgccacaatg acctgatttg ccagtttggt
     2641 gacgttacct gcaccgattt ccccggtatg caccacggaa cccgccatcg ctttcatcaa
     2701 atcatagtat ttgtcgaaaa tagccttgtc gccgcccacc atcactgaca gcgtaccgtc
     2761 gatggctttc ggttcaccgc cgctcaccgg agcatccagc atatcaatgc ctttcgcttt
     2821 cagcgcttcg ctgatttcac ggcttgccag cggtgcgata gaactcatat cgatcaatac
     2881 cgtacctggc ttcgcgcctt caataatgcc attctcaccc agcgccacct ctttcacatg
     2941 aggggagttt ggcagcatgg ttatgatgac gtcgcactgt tcagcgatcg ctttagccgt
     3001 agcggctgtt tctgcacctg cagcaatcac gtcagcaata gcttctgggt tacggtcagc
     3061 aaccaccagc gagtaacctg ctttcaaaag gtttttactc attggtttac ccataatccc
     3121 aaggccaata aaaccaactt tcatagtcat atcaatcatc tctcttattg cggtggtggt
     3181 tattttttaa aggtatcagc cagtttctga gtggcagagc ggaagacgcc gagatcgctg
     3241 ccgacggcca caaacgtcgc gccccattcc agataacgac gcgcatcagc ctcgactggt
     3301 gcgaggatgc cgctgggttt gccgtgcgcg ctggcacggt taaaaatgtg ctgaattgct
     3361 ttttgtacat ccgggtgcga ggcattgccg agatggccta atgccgcagc cagatcgctg
     3421 gggccgacga agatgccgtc tacaccttcg gtagcggcaa tagcatcgac gttatctacg
     3481 ccctgctgac tttctatctg gaccagaata gtgatgttct tgttcgactg agcgaaataa
     3541 tccgccacgg tgccaaacat attggcgcgg tgagaaacgg agacgccgcg aatgccttcc
     3601 ggtgggtaac gagttgatgc caccgcctgc tctgcttcct cttttgtttc tacaaaagga
     3661 atcaggaagt tatagaaacc gatatccaga agacgcttaa taattaccgg ctcgttggtc
     3721 ggcactcgca ctactggcgc gctggcgctg cctttcaggg ccattaactg cggaataaac
     3781 gtggagatat cgtttggcgc atgttcacca tccagcacca gccagtcaaa cccagccaaa
     3841 ccaagaactt cagtgctaat cgggttagag agagctgacc agcaaccaat ttgtacctgt
     3901 ttcgcagcca gtgcggcttt gaatttattc gggaaaacat cgttattcat cgcttatacc
     3961 tttgcttatt tctgcaattc catacgttta atgtcgccga ctacgaagag gtagcagacc
     4021 atcgccatca gcgctgaaca tcccacaaaa accagtgctg cattgaagga gtgcagttca
     4081 cttaccaggt agccaatcac cagtggagtg acaatggagg caacattgcc aaagacgtta
     4141 aagacgccgc cgcagaggcc aacaatctct ttcggcgcgg tgtcagaaat cactggccag
     4201 cccagcgcac caaatccttt accaaagaaa gccagcgcca tcagcatgac caccagcgtg
     4261 gtgttgttgg tgtagttaca taagatgatg gtggaagcca gcaacattcc cagcacaatt
     4321 ggtagcttac gagccagggt cagggacaaa ccgcgtttga tcagataatc cgagaagaca
     4381 cctcccagca cgccgcccgc aaagccacac agtgccggaa tcgaggcgac cagacccact
     4441 ttcagaatcg acatgccttt ttcctgcacc agataaattg ggaaccaggt gaggaagaac
     4501 caggtgatag tgttgataaa atattgtccg aaaaatacgc ccagcatcat gcggttagag
     4561 agcaattgct tgatgtaatg cagtttgggt ccgcttgctg ccgcactgcc cggctttttg
     4621 tggtccatat cgaccaccgc gccattttcc gagataaact tcagctcttc cgcagacata
     4681 cgtgggtgat ctgtcggatt atgaatcaac ttgatccaca gcgccgtcag cacaaaacca
     4741 atcaccccca taacggtaaa gacgtgctcc cagccccagg cgaaagtcag ccagccaagc
     4801 agcggcgaaa agagcgccaa cgagaaatat tgcgccgagt taaagatggc ggaggcagta
     4861 ccacgttctt tcgtcgggaa ccaggcggcg acaatacggg cgttcgccgg gaatgatggc
     4921 gcttccgaga accccagcat aaatcgcata aagaacatgg agatcccagc ccaggccagc
     4981 ggaaacatat cgacaaagcc ttgcaggaag gtgaacagcg accagaaaaa gaggctgtaa
     5041 gtgtaaactt tcttcgagcc aaacttatca agcagccagc cgccgggaat ttgcatcagc
     5101 aagtaggccc agccaaaagc agagaagatg taacccatcg aaaccgcact taactgcaac
     5161 tcttttgcca cttcggtacc agcaatagac agcgttgcac gatcggcgta gttaacggcg
     5221 gtaacaataa aaataatcag taatattaaa tagcgggtat gcacgccttt ctttttttcg
     5281 tcaacggtgt ccagcatcat tttatttacc tcgggtactt atgctgattt ttattattat
     5341 ggggaaggtg ttatttatga gtttcattta tgccgttacg acaatgaact cgggaattag
     5401 tataagcagc gcgagaataa taatcattgt gcaaatgcta atttaattag tactatttaa
     5461 atattatttt gagcatatgc acataaggtt gcgcgctaaa gcacagattt gcgctttacc
     5521 ttgccgggag gtactgcaat ccctgaaatg attgacattg atcacatttc tgcatttaaa
     5581 ctcctgacat tcttatttca cccaatgaag ttatttattt ttaaatgaga ccaggtcctc
     5641 attttaatag tccctggctg gagaatattg cacaatggcc aacatcgaaa tcagacaaga
     5701 aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa ttattgttaa
     5761 tgataatggc ctgaaagcag gaacgcgttt tccggatggg ctggaattaa ttgaacatat
     5821 tccccagggg cataaagtcg cattgctgga cattccggct aatggtgaaa ttattcgtta
     5881 tggcgaggtg attggttacg ccgtgcgcgc aatcccacgc ggaagctgga tcgacgaatc
     5941 aatggttgta ctaccggaag cgccgccgtt acatacgctg ccactggcaa ccaaagtccc
     6001 ggaaccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg ccgatggcag
     6061 cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg caggcgtggt
     6121 ggactacgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga acgtcgatgg
     6181 cgtggtgggg ctgaatcatt tgtacggttg tggcgtggcg attaacgcac ccgcggcagt
     6241 tgtacctatt cgtaccattc acaatatttc gctgaaccct aactttggcg gcgaagtaat
     6301 ggtgattggt ctgggttgtg aaaagttgca gcctgaacgc ctactgactg gaacggatga
     6361 tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg aaaagcatgt
     6421 cggttttcag tccatggtcg aggatatttt gcaggtcgcc gaacgccatc tacaaaaact
     6481 gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg gttgtcggca tgcagtgcgg
     6541 tggcagcgat gcgttttctg gcgtaacggc aaacccggcg gttgggtatg cgtctgatct
     6601 attggtgcgc tgcggcgcaa cggtgatgtt ttcagaagtc acagaagtgc gtgacgcgat
     6661 acatctgctg acaccacgcg ctgtgaacga agaggtcggc aaacggctgc tggaggagat
     6721 ggagtggtac gataactatc tcaatatggg aaaaaccgat cgcagcgcca acccttcgcc
     6781 ggggaacaag aaaggcggtc tggcaaatgt ggtggagaag gcactcggct ccattgctaa
     6841 atcgggtaaa agtgcaattg ttgaagtgct gtcgcccggc caacgcccga ctaaacgcgg
     6901 attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac aggtggcttc
     6961 gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc tgatggcggt
     7021 acccgtcatt aaaatggcga cccgcaccga gctggcgaac cgctggtttg atttaatgga
     7081 tattaacgcg ggcactatcg ctaccggcga agaaaccatt gaagaggtgg gctggaagtt
     7141 gttccacttt attctcgacg tcgccagcgg gaagaagaaa accttctcag atcaatgggg
     7201 actgcataac cagctggcgg tgtttaaccc agcaccggtg acctgatttc ttttggaacg
     7261 tccctcgcaa aacatggcct tagtgccatg tttttattgt ttaaagtccc cacgtccatt
     7321 aataatgcat ttgcattacc ttagttcaag cttataattt gagcagaaaa caggctgtaa
     7381 aaggacagtg aatcatgccc gctaatgctc gctctcacgc tgtactgacc actgaatcaa
     7441 aggtcacgat acgcggacaa acaactatcc ccgcgccagt gcgtgaggcc ttaaaactga
     7501 agccaggcct ggacagcatt cattacgaaa ttctgcctgg tgggcaagta tttatgtgcc
     7561 gactgggaga tgaacaggag gatcatacta tgaatgcatt tttgcgtttt ctggatgcag
     7621 atatccagaa caacccgcaa aaaactcgtc cattcaacat tcaacaagga aagaaacttg
     7681 tcgctggcat ggacgtcaac attgatgatg agattggcga cgacgaataa tggattttcc
     7741 acaaagggtt aatggttggg cgctatatgc tcatccctgt tttcaggaaa cctacgacgc
     7801 tttagttgcc gaagtcgaga cattaaaggg aaaagatcct gaaaattatc agagaaaagc
     7861 cgccacaaag ttattggcgg tagtccataa agtgattgag gagcatatca cggtcaatcc
     7921 atcatcaccg gcattccgtc atggcaagtc gttaggctct gggaaaaata aagactggtc
     7981 acgggtaaaa tttggtgctg gtcgttatcg tctcttcttt cgttatagtg aaaaagagaa
     8041 agtcatcatt ctgggatgga tgaacgatga aaacacgctg cgcacctacg gtaaaaaaac
     8101 agatgcctat accgtattca gcaaaatgtt aaaaagagga catcctcctg ccgactggga
     8161 aaccctcacc cgagagacag aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc
     8221 attcgtgttg accagcgggc accctttctc actccccgac cagaatcact tcaaccccag
     8281 cctttcgcag tccttccaga ctatccgcag gaatgccttc atcaacaatg atcatgtcga
     8341 tacgttgagt atcaatgatc ttatgtaaac tggaacggtt gaacttactg gaatcggtga
     8401 ccacgatgat ccgttccgca acttcgcaca tccgacggtt taaacgggct tcatcttcat
     8461 tatgcgtgct gacgccgcgc tccagatcga tcgcatctac accaagaaac agcatatcga
     8521 agtggtaatt ttgcagcgat tgctcagcct gatcaccgta aaaagattgc gactgacggc
     8581 gcaaatgccc cccggtcatc agcagctcaa cgccttccgc ttccagcaac gcattagcca
     8641 cgttcatacc gttggtcatc gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct
     8701 caaaagtggt ggtcccggaa tcgaggatta cccgatgacc tggctgaatc aactcaacgg
     8761 cagctttcgc aacgctgcgt ttcattgcgg tgttcagcgc gcttttatct tccactgatg
     8821 gctcgactga cggcgtcgtg ctatcgcaga tcaacgcgcc gccataggca cgcacagcga
     8881 tcccctgctt ttccagaaac gccagatcgt tgcggatcgt cacagtagat acgccataca
     8941 atgccgacag atcgttaacc tgcacacttc cttgctggcg caggcgttga atgatctgct
     9001 ctcgtcgctc actggtgcct gtcactcgct tctcgcctga agcgtcggta ttactcatag
     9061 taagtccttt cgtaaaactt tcgtttcatt tcgttttgcc tattaacgcc tttctattaa
     9121 gcaaatgcaa gcccacctta cccattggcg caagctactc tcgtttcagt ggctttcact
     9181 atgtttcttt tgtgaatcag atcagaaaac tattatcttt cgttttattt ttatctcacc
     9241 atggcgcagt atcaactgaa acaaaacgaa agattaatat cgcagtaatc tgaactggag
     9301 aggaaagtga aacatctgac agaaatggtg agacagcaca aagagggcaa aacaaatggg
     9361 atttatgccg tttgttccgc acatccgctg gtgctggaag ctgcaatccg ctacgccagt
     9421 gcaaaccaaa cgccgttact gattgaagca acctccaatc aggtggacca gttcggcggt
     9481 tataccggaa tgacgcccgc cgattttcgc ggctttgttt gtcagctcgc cgactcgttg
     9541 aatttcccac aggatgcgtt gattctgggt ggcgaccatc tggggccaaa ccgctggcaa
     9601 aacctgcctg ccgctcaggc aatggccaat gccgatgatt tgattaaaag ctacgtggcg
     9661 gcaggattca aaaaaattca ccttgattgc agcatgtcct gtcaggacga tccgattccc
     9721 ttaactgatg acatcgtggc cgaacgcgcc gctcgtctgg cgaaagtggc ggaagaaacc
     9781 tgccgtgaac attttggcgc ggccgatctg gagtatgtca ttggtaccga agtaccggta
     9841 cctggcggcg cgcatgaaac cttaagcgag ctggcggtca ccacgccgga tgccgcccgc
     9901 gccacgctgg aagcccatcg tcacgccttt gaaaagcaag gtctgagtgc catctggcca
     9961 cgcatcattg ctctggtggt acaacccggc gtcgaattcg atcacaccaa cgttattgat
    10021 tatcagcccg tcaaagcgac cgccttaagc cagatggtcg aaaactacga aacgctgatt
    10081 ttcgaagcgc actctaccga ttaccaaacg ccgcaatcgc tgcgccagct ggtgattgac
    10141 cactttgcca ttctgaaagt tggcccggcg ctgaccttcg ccctgcgtga agctctgttc
    10201 tctctggcgg cgattgaaga agaactggtg ccagcgaaag cctgttccgg tctgcgtcag
    10261 gtgctggaaa acgtgatgct cgaccgcccg gaatactggc aaagccacta ccacggtgac
    10321 ggcaacgcgc gtcgtctggc gcgtggttat agctactcgg atcgcgtgcg ctattactgg
    10381 ccggacagcc agattgatga cgctttcgct catctggttc gtaatctggc ggattcacca
    10441 attccgctac cgctgatcag ccagtatttg ccgctgcaat acgtgaaagt tcgctccggc
    10501 gagctgcagc caacaccacg ggaactcatt atcaaccata ttcaggacat cctggcgcag
    10561 taccacacag cctgtgaagg ccaataagca aaacaaagag gaacacgcta tgccaaatat
    10621 tgttttaagc cggattgatg agcgtttgat tcacggtcag gtcggcgttc agtgggtcgg
    10681 atttgcgggg gcaaatctgg tgctggtagc caacgatgag gttgccgaag atccggtaca
    10741 acaaaacctg atggaaatgg tactggcaga agggatcgcc gtacgtttct ggacgctgca
    10801 aaaagtcatc gacaacattc atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa
    10861 aacacctgcc gatttcctga cgctggtaaa aggtggcgtt ccggtgaatc gcattaacgt
    10921 tggcaatatg cactacgcca atggcaagca acaaatcgcc aaaacggttt ctgtggatgc
    10981 gggtgatatc gcagcattta acgacctgaa agccgctggg gtggaatgct tcgttcaggg
    11041 cgtcccgaca gagcctgcag tggacctctt taaattactt tgagggattc atcatggaaa
    11101 tcagtctgtt gcaggcattc gcgttgggca ttatcgcctt tatcgctggc ctggatatgt
    11161 ttaacggcct aacccatatg caccgcccgg tggtcctcgg cccgttggtc gggctggtac
    11221 ttggcgatct gcataccggt attttaaccg gcggtacgct ggaactggtg tggatggggc
    11281 tggccccgct ggcaggcgca caaccgccta acgtgattat cggtactatc gtcggcacgg
    11341 cgtttgccat tactactggc gtgaaacccg atgtcgcggt cggtgtcgcc gtacctttcg
    11401 ctgtcgcagt acagatgggg attaccttcc tgttctcggt gatgtccggc gtgatgtccc
    11461 gctgcgaccg gatggcggag aacgccgata ctcgcggcat tgaacgcgtg aactatctgg
    11521 cattgctggc actcggcact ttctattttc tctgcgcttt cctgcctatc tacttcggcg
    11581 cggaacatgc aaaaaccatc attgatgtcc tgccgcaacg attaattgat ggcctcggcg
    11641 tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa
    11701 acgtctacat cccctacttc atcctgggct ttgttgccgc ggcctggctc aagttaccgg
    11761 tgttggctat tgctgccgct gcgctggcga tggcgctgat cgacctgctg cgtaaatctc
    11821 ctgaaccgac tcaacctgcg gcacagaaag aggaatttga agatggcatc taatcaaacc
    11881 accctgccga acgtcagcga aaatgaagaa acactgctga ctggcgtcaa tgaaaacgtg
    11941 tatgaagatc agagcattgg cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg
    12001 cgttccatgt tgttacaggc ttctttcaac tacgaacgta tgcaggcttc cggctggctg
    12061 tacggcctgc tgccagcact gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc
    12121 atgaaggggc atatgggttt cttcaatacc catccgtttc tggtgacgtt tgttatcggc
    12181 attatccttg cgatggagcg ttctaagcag gacgttaaca gtattcagag caccaaaatt
    12241 gccgtcggtg cgccgctcgg cgggattggc gatgcgatgt tctggctaac gctactgccg
    12301 atttgtggcg ggataggtgc aagcctcgcg ctacaaggct ctattcttgg cgctgtcgtc
    12361 tttattgtgc tgttcaacgt ggtacacctg gggctgcgtt ttggtctggc gcattatgct
    12421 taccgcatgg gcgtggcggc gattccactc attaaagcaa ataccaaaaa agtcggccat
    12481 gcggcatcta tcgttgggat gacggtaatc ggcgcgctgg tggcaaccta tgttcgttta
    12541 agcaccacgc tggaaatcac agcgggcgac gcagtggtta agttacaggc tgatgttatc
    12601 gacaaactga tgcctgcctt cttaccgctg gtctacaccc tgaccatgtt ctggctggta
    12661 cgccgcggct ggagtccgct gcgcctgatt gcagtgacag tggttctcgg catcgtcggt
    12721 aaattctgcc atttccttta aatacaatga ggtattagat gttaagtatt attttgacag
    12781 ggcatggcgg gtttgccagc ggtatggaaa aagcaatgaa gcaaattctc ggtgagcaat
    12841 cgcagtttat cgccatcgat tttccggaaa cctcaagcac cgcgcagctt acatcgcagc
    12901 ttgaagaggc catcgcccaa ctggattgtg aagacggcat tgtttttctg acggatttgc
    12961 tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag
    13021 tgatcaccgg cacaaatttg caactattac tggaaatggt gctggaacgc gaagggttaa
    13081 gcggtgaaga gttccgtgtg caggcgctgg agtgtggaca ccgtgggcta accagtctgg
    13141 tggacgaact tggtcgctgc catgaggaat gtccggtcga ggaaggaata tgacacacgt
    13201 tctgcgcgcc agaaggctgc tgactgaaga gggatggctc gatgaccatc agttgcgtat
    13261 tgctgacggt gtaatcgcag caatcgaacc gattccagtg ggcgtgactg aacgcgatgt
    13321 ggaactgctc tgccccgctt acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt
    13381 aatggatgac gcgccggatg cgctcgacaa gctggcaatg cacaaggcac gcgaaggtgt
    13441 cggcagttgg ttaccgacca ccgtaaccgc gccgcttaat accattcatg cggcgctgaa
    13501 acgtattgct ctacgctgcc agcgtggcgg acctggtgcg caagtgctgg ggagttatct
    13561 cgaaggaccg tacttcacgc cgcagaataa aggcgcgcat ccaccggagc tgtttcgcga
    13621 gcttgaaatt gccgagctgg atcaattgat tgccgtttct cagcatacct tgcgcgtggt
    13681 agcgctggca ccagaaaaag aaggggcatt gcaggccatc cgccatctta aacagcaaaa
    13741 cgtacgggtg atgctggggc atagcgcggc gacctggcaa caaacccgtg ccgcgtttga
    13801 tgctggtgcc gacggcctgg tgcattgcta taacgggatg acaggtttac atcaccgcga
    13861 accgggaatg gctggcgcgg gattaacgga caagcgcgcc tggctggaac tgatagccga
    13921 tggtcatcat gtacatccgg cggcaatgtc gctctgttgt tgctgtgcga aagagaggat
    13981 cgtactgatc accgacgcga tgcaggcagc cgggatgccg gatggtcact atacgttatg
    14041 tggtgaagaa gtgcagatgc gcggtggcgt tgtccgtacc gcgtccggtg ggctggcggg
    14101 cagtacgctg tctgtcgatg cggcagtgcg caacatggtc gagttgacgg gcgtaacgcc
    14161 tgcggaagcc atccatatgg cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt
    14221 tctgggatcg cttaaaccgg gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca
    14281 tgtgcaacaa atctggattc agggtcaatt agcttcgttt tgatggtttg ctcctttatt
    14341 aggccttcac ttcccccgta aggcctttct ttttctttcg ttttgatctg tgcagcggtg
    14401 tcggatgcga cgctaatgcg tcttatccga cctacggttg gtgagcgtaa ggccggataa
    14461 agtatttacg ccgcatccag caatcccttt tacttccctt accttttctt tcaacgatca
    14521 cagatttcgt tttatttcct ttttctccat tgaactttca gtttcttttc tatagatttt
    14581 aaccaacgaa agaaatcacc aagtgaaatg aaacgaaagg caagcgaaag cgacaacgcc
    14641 gacgtcaagt tcatcagact aaggattgag ttatgccaga aaattacacc cctgctgctg
    14701 ccgcaaccgg tacatggact gaagaagaga tccgccatca gcctcgcgca tggatccgtt
    14761 cactcaccaa cattgacgcg ctacgttccg cgctcaataa cttccttgaa ccgttactgc
    14821 gcaaagagaa tctgcaaatc atcctgaccg gagccggaac atcggcattt atcggtgaca
    14881 tcatcgcgcc gtggctcgcc agccataccg gtaaaaactt cagcgccgta ccgaccaccg
    14941 atctggtcac taatccgatg gactacctga atccagctca tccgctacta ttgatctcct
    15001 tcggtcgatc cggcaacagc ccggaaagcg tcgccgccgt gaaactggca aatcaatttg
    15061 taccagaatg ctatcacctg ccgatcacct gcaacgaagc gggcgctctt taccaaaacg
    15121 cgatcaacag cgacaacgcg tttgccctgc tgatgcccgc agaaacgcac gatcgcggct
    15181 tcgcgatgac cagcagcatt accaccatga tggccagctg cctcgcggtt ttcgcacctg
    15241 agatgatcaa cagccaaacc ttccgcgacg tggcggatcg ttgccaggcg atcctgacct
    15301 cactgggcga tttcagcgaa ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc
    15361 tcggcagcgg tggcttacag ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga
    15421 cagcgggtaa actggcggcc ttctatgatt ccccgaccgg attccgtcat ggcccgaaat
    15481 cgctggtcga taacgaaacg ctggtggtgg tgtttgtctc cagccaccct tacacccgtc
    15541 agtatgatct tgatctgctg gcagaactcc gccgcgataa ccaggcaatg cgcgtaatcg
    15601 ccatcgccgc ggaaagcaac gacgttatta ccgccggtcc acatatcatc ctgccgccgt
    15661 cccgtcactt tatcgacgtt gagcaggcat tttgcttcct gatgtacgcc cagacatttg
    15721 cactgatgca gtcgctgcac atgggtaata cgccggatac cccatcagcc agtggcaccg
    15781 ttaaccgcgt ggtgcaaggc gtaatcattc atccgtggca ggcataagag gatcgcatta
    15841 tgagcattat ctccaccaaa tatctgttac aggacgccca ggccaatggc tacgcggtgc
    15901 ctgcttttaa cattcataac gccgagacga tccaggcgat cctcgaagtg tgcagtgaaa
    15961 tgcgatcgcc ggtgatcctc gccgggacgc cggggacctt taagcatatc gcgctggaag
    16021 agatctacgc cctgtgtagc gcctattcca cgacctataa catgccgctg gcactgcatc
    16081 ttgatcacca cgaatcgctg gatgatattc gccgtaaagt ccacgcaggc gtgcgcagcg
    16141 cgatgatcga cggcagtcac ttcccgtttg ccgagaacgt gaagctggtg aaatcggttg
    16201 tcgacttctg ccactcccag gattgcagcg tggaagcaga actgggccgc ctcggcggtg
    16261 ttgaagatga catgagcgtt gacgccgaaa gtgcattcct gaccgatcca caagaagcta
    16321 aacgctttgt cgaactgact ggcgtcgaca gcctggcggt agcgattggt acggcgcacg
    16381 gcttatacag caaaacgccg aagattgatt tccagcggct ggcggaaatt cgtgaagtgg
    16441 tcgatgttcc tctggtgctg catggtgcca gcgatgtccc ggatgaattt gtccgtcgca
    16501 ctattgaact tggcgtaaca aaagtgaacg ttgccacaga attaaaaata gccttcgctg
    16561 gcgcggttaa agcctggttt gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc
    16621 gcgtcggaat ggatgcgatg aaagaagttg tcagaaataa aattaatgtc tgtggttcag
    16681 cgaatcgaat ttcagcataa tcattagttt tttatgattt atcccaatgt acttcccgat
    16741 taatatcggg gagtg
//
LOCUS       DADSZD010000097        16712 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.098, whole genome shotgun sequence.
ACCESSION   DADSZD010000097 DADSZD010000000
VERSION     DADSZD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.098"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..646
                     /gene="ompL"
                     /locus_tag="J1Z28_002876"
     CDS             <1..646
                     /gene="ompL"
                     /locus_tag="J1Z28_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBA7717693.1"
                     /translation="SVFAGAYVENREAYNLASDQGEVMLRVGYNFDMGAGIMLTNTYT
                     FQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGSGGAVYLDVNYKFTPWF
                     NLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKFSYTFEPHYFMRVNDFN
                     SSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHREQNQIRIGTKYFF"
     gene            complement(748..2571)
                     /gene="typA"
                     /locus_tag="J1Z28_002877"
     CDS             complement(748..2571)
                     /gene="typA"
                     /locus_tag="J1Z28_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBA7717694.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2944..4353
                     /gene="glnA"
                     /locus_tag="J1Z28_002878"
     CDS             2944..4353
                     /gene="glnA"
                     /locus_tag="J1Z28_002878"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBA7717695.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            4527..5576
                     /locus_tag="J1Z28_002879"
     CDS             4527..5576
                     /locus_tag="J1Z28_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HBA7717696.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            5588..6997
                     /gene="glnG"
                     /locus_tag="J1Z28_002880"
     CDS             5588..6997
                     /gene="glnG"
                     /locus_tag="J1Z28_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBA7717697.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLTWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     SPSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            7109..7219
                     /gene="yshB"
                     /locus_tag="J1Z28_002881"
     CDS             7109..7219
                     /gene="yshB"
                     /locus_tag="J1Z28_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBA7717698.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(7452..8825)
                     /gene="hemN"
                     /locus_tag="J1Z28_002882"
     CDS             complement(7452..8825)
                     /gene="hemN"
                     /locus_tag="J1Z28_002882"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBA7717699.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RKVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(9014..9523)
                     /gene="yihI"
                     /locus_tag="J1Z28_002883"
     CDS             complement(9014..9523)
                     /gene="yihI"
                     /locus_tag="J1Z28_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBA7717700.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            10105..10737
                     /locus_tag="J1Z28_002884"
     CDS             10105..10737
                     /locus_tag="J1Z28_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBA7717701.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(11119..13905)
                     /gene="polA"
                     /locus_tag="J1Z28_002885"
     CDS             complement(11119..13905)
                     /gene="polA"
                     /locus_tag="J1Z28_002885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBA7717702.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13907..14149
                     /locus_tag="J1Z28_002886"
     CDS             13907..14149
                     /locus_tag="J1Z28_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717703.1"
                     /translation="MSVPVYQIMGKGCHRQDENMSFSPLFVKIFCRSSGSLANRHVDN
                     FVHKLFTSLLIYYHAFYSMVFVFVFHAVKNKAIPNN"
     gene            14269..15201
                     /locus_tag="J1Z28_002887"
     CDS             14269..15201
                     /locus_tag="J1Z28_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA7717704.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSSFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQEK"
     gene            complement(15242..16672)
                     /locus_tag="J1Z28_002888"
     CDS             complement(15242..16672)
                     /locus_tag="J1Z28_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBA7717705.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGPFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGLMLQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3993 a         4248 c         4098 g         4373 t
ORIGIN      
        1 ctcggtattc gctggcgcat acgtcgaaaa cagggaagca tataatctcg cctccgatca
       61 gggtgaggtt atgctacgtg tgggttataa cttcgatatg ggcgcgggta ttatgctaac
      121 caatacctac acctttcagc gagaagatga actcaaacat ggctataatg aaattgaagg
      181 ttggtatcct ttatttaaac ctaccgataa attaaccatc cagcccggtg gcttaattaa
      241 tgacaagagt atcggttcgg gtggtgctgt ttatctggat gttaactaca aatttactcc
      301 gtggtttaac ctgacggtgc gtaatcgcta taaccataat aattacagct ctacagattt
      361 gaacggcgaa ttggataata acgacaccta tgaagttggc acctactgga actttaaaat
      421 aacagaaaaa ttttcctata catttgaacc acattacttc atgcgagtga atgactttaa
      481 tagtagcaac gggaaagatc atcattggga aatcactaac accttccgtt accgtattaa
      541 tgaacactgg cttccttatt tcgaattgcg ctggttagac cgcaacgtcg agccgtacca
      601 ccgcgagcaa aaccagattc gtatcggaac gaagtatttc ttctgatgta aaaattcctg
      661 atgcgatgct ggcgcatctt atcaggtcta caaaagccaa aaaaaacctg ccatcgctgg
      721 caggtttttt atgactaaaa aacgaaatta atcgtctttc ggtgcgcggt tggcgcgacg
      781 acgatcgttt tccgtcaggt gacgtttacg aatacgaata gaggtcggag tcacttctac
      841 cagttcgtca tcatcgatga actccagagc ttgttccaga gtcatgcgga taggcggaac
      901 cagaacaacg gcttcgtcag taccggaagc acgcatgttg gtcagtttct taccggtcag
      961 gcagtttaca gtcaggtcgt tagagcggct atgaataccg ataatctgac cttcgtaaac
     1021 ttctgcaccg tgaccgagga acagcttacc acggtcctgc agaccgaaca gcgcgaacgc
     1081 gaccgcttta ccctgaccgt tagagatcag tacaccgttc tgacgctgac ccacttcgcc
     1141 cggacgtacg tcgtcgtagt ggctgaaggt ggagtacagc agaccagtac cggaagtcat
     1201 ggtcatgaac tcagaacgga agccaatcag accacggctt gggatcacgt agtcgagacg
     1261 tacgcggcct ttaccgtctg gattcatgtt tttcaggtcg cctttacgtt cgcccagcgc
     1321 ctgcatcaca gaaccctgat gctgttcttc aacgtccagc gtcacgtttt catacggctc
     1381 ttgtttacga ccgtcgattt cacggaagat tactttcgga cgggataccg ccagttcgaa
     1441 accttcacga cgcatgtttt cgatcagaac agacaggtgc agttcgccac gaccagaaac
     1501 gcggaacgca tcggcgtctt cggtttcttc tacgcgcagc gcaacgttgt gtaccagttc
     1561 tttgttcaga cgatccagga tctgacgaga cgttacgaac ttaccttctt taccgcagaa
     1621 cggcgaggtg ttaacgcaga agaacataga aacggtcggc tcatcaacgg agagtgccgg
     1681 cagcgcttca acgttttgcg tgtcgcaaac ggtgtcagaa atgttcagtt cgccaaggcc
     1741 cgtgatcgcc acgatatcgc cagcttccgc cagatcggtt tcgatacgtt ccagaccgag
     1801 gtggcccagc actttaccga ctttcgcgtt gcgggttttg ccttcgctat cgatgatagt
     1861 aacctgctgg ttcggcttca ctttaccgcg cttgatgcgg ccaatgccga taacgccaac
     1921 atagctgttg taatcgagct gagaaatctg catctggaac ggaccgtcaa ggtcaacgtc
     1981 cggcgcagga acgtggtcaa caatcgcctg atacagcggg gtcatgtctt ccgccatatc
     2041 ttcgtggtcc agacccgcga taccgttcag cgcagaagcg taaacgatcg ggaagtccag
     2101 ctgctcgtcg gtcgcgtcga ggttaacgaa cagatcgaat acctgatcca caacccaatc
     2161 aggacgcgcg ccagggcggt caactttgtt gataacaaca atcggcttca ggccgtaagc
     2221 aaacgctttt ttggttacga agcgcgtttg cggcatcggg ccgtcaaatg cgtcaaccac
     2281 cagcagcact gagtctacca tggacattac acgttcaact tcaccaccga agtcggcgtg
     2341 ccccggggta tcaacgatgt tgatacggta atcattccat ttgatagcgg tgtttttcgc
     2401 gaggatggta atcccacgct ctttctccaa atcgttggag tccatcacgc gctcttgggt
     2461 ttcggcacga gagtcgaacg taccggattg ttggagcagc ttgtctacca gggtggtttt
     2521 accatggtct acgtgcgcga tgatggcgat attacgcaat ttttcgatca caactttgcc
     2581 tcaggcatta gaaatagcgc gttattgtac acggattaat cgcactacaa aacaggatca
     2641 caaacatcct ccgcaaacaa gtattgcaga gtccctttgt gatcgctttc acggagcata
     2701 aaaagggtta tccaaaggtc attgcaccaa tatggtgctt aatgtttcca ttgaagcact
     2761 atattggtgc aacattcaca tcatggtgca gcccttttgc acgatgttaa gcatgataac
     2821 gccttttagg ggcaatttaa aagttggcac agttttcgct ttatcttttt tacggcgaca
     2881 cggccaaaat aattgcagat ttcgttacca cgacgaccat gaccaatcca ggagagttaa
     2941 agtatgtccg ctgaacacgt actgacgatg ctgaacgagc acgaagtgaa gtttgttgat
     3001 ttgcgcttca ccgataccaa aggtaaagaa cagcacgtca ctatccctgc tcatcaggtg
     3061 aatgccgaat tcttcgaaga aggcaaaatg tttgacggct cctcgattgg cggctggaaa
     3121 ggcattaacg aatccgacat ggtgctgatg ccagacgcat ccaccgcagt gattgacccg
     3181 ttcttcgccg attctaccct gattatccgt tgcgacatcc ttgaacctgg caccctgcaa
     3241 ggctatgacc gtgacccgcg ctctattgcg aagcgcgctg aagattacct gcgttccact
     3301 ggcattgccg acaccgtact gttcgggcca gaacctgaat tcttcctgtt cgatgacatc
     3361 cgtttcggat cttctatctc cggttcccac gttgctatcg acgatatcga aggtgcatgg
     3421 aactcctcca cccaatatga aggtggtaac aaaggtcacc gtccggcagt gaaaggcggt
     3481 tacttcccgg ttccgccggt agactcagct caggatatcc gttctgaaat gtgcctggtg
     3541 atggaacaga tgggtctggt ggttgaagct catcaccacg aagtcgctac cgcaggtcag
     3601 aacgaagtgg ctacccgctt caataccatg accaaaaaag ctgacgaaat tcagatctac
     3661 aaatatgtcg tgcacaacgt ggcgcaccgt ttcggtaaga ccgcgacctt tatgccaaaa
     3721 ccgatgttcg gtgataacgg ttccggtatg cactgccaca tgtcgctgtc taaaaacggc
     3781 gtcaacctgt ttgctggcga caaatacgca ggtctgtctg agcaggcact gtactacatt
     3841 ggcggcgtaa tcaaacacgc taaagcgatt aacgccctgg caaacccgac caccaactct
     3901 tacaagcgtc tggtcccggg ctacgaagca ccggtaatgc tggcttactc tgcgcgtaac
     3961 cgttctgcgt ctatccgtat tccggtggtt tcttctccga aagcacgtcg tatcgaagta
     4021 cgtttcccgg acccggcggc taacccgtac ctgtgctttg ctgccctgct gatggccggt
     4081 cttgatggca tcaagaacaa gatccatccg ggcgaagcca tggacaaaaa cctgtatgac
     4141 ctgccgccag aagaagcgaa agagatccca caggttgcag gctctctgga agaagcactg
     4201 aacgaactgg atctggaccg cgagttcctg aaagctggtg gcgtgttcac ggatgaagca
     4261 atcgatgcgt acatcgctct gcgtcgcgaa gaagatgacc gcgtgcgtat gactccgcat
     4321 ccggtagagt ttgagctgta ctacagcgtc taatagttga agttgtacta cccggcgcaa
     4381 caacgccggg atttagttgc cgtggaaact ttcagcccat ctctgcatgg gcttttttct
     4441 ccgtcaattc tctgatgctt cgcgcttttt atccgtaaaa agctataatg cactaaaata
     4501 gtgcaacctg ttcaggagac tgctttatgg caacaggcac gcagcccgat gctgggcaga
     4561 tcctcaactc gctgattaac agtattttgt taatcgatga caacctggcg atccattacg
     4621 ccaaccccgc cgcgcaacaa ctgctcgccc aaagctcccg caaattgttt ggtacaccgt
     4681 taccggaact gttgagctac ttctcattaa atatcgagct gatgcaagaa agtctggagg
     4741 cggggcaagg ttttaccgat aacgaagtga cgctggtcat cgacgggcgc tcgcatatcc
     4801 tttctgtgac ggctcagcgt atgccggacg gcatgatcct gctggagatg gcaccgatgg
     4861 ataaccagcg ccgcttaagt caggaacagc tacagcacgc ccagcaggtt gctgcccgtg
     4921 atttagtgcg cggcctggca catgagatta aaaatccgct tggcggttta cgtggcgcgg
     4981 cgcagctact cagcaaagca ttacctgacc cgtcactact cgaatatacc aaagtgatta
     5041 tcgaacaggc ggaccggctg cgaaatctgg tcgaccgtct gttggggccg cagctgcccg
     5101 gtacgcgcat taccgaaagt attcacaaag tggctgaacg cgtggtgacg ctggtgtcga
     5161 tggaactgcc gaacaacgtg cggttgattc gggattacga ccccagcctg ccggaactgg
     5221 cgcacgaccc ggatcaaatt gaacaggtct tgctgaatat tgtgcgcaat gcgctacagg
     5281 cgctggggcc ggagggtggt gaaatcattc tgcgtacccg cacagcgttt caactgacct
     5341 tacacggcga gcgctatcgg ctggcggcgc ggattgatgt ggaagataac gggccgggca
     5401 ttccgcctca tttgcaggat acgctgtttt acccgatggt cagcggccgc gaaggtggca
     5461 ccgggcttgg cttatccatc gcccgtaatt tgattgatca gcattcaggc aaaattgaat
     5521 ttaccagttg gccaggtcat accgagttct cggtttacct gcctatcagg aaataaaggt
     5581 gacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca tccgttgggt
     5641 gcttgaacgt gcgctcgctg gagcgggttt aacctgtacg acatttgaga acggcgcgga
     5701 agtactggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata tccgtatgcc
     5761 gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccga tgcttccggt
     5821 catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc aacaaggggc
     5881 atttgattat ctgcccaaac cgtttgatat cgacgaagcc gtggcgctgg ttgagcgcgc
     5941 catcagtcat taccaggaac agcagcagcc gcgtaatgtt cagcttaacg gcccaacgac
     6001 cgatatcatc ggtgaagcgc cagccatgca ggacgtgttc cggattatcg gtcggctttc
     6061 gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag aactggtcgc
     6121 tcatgccctg catcgccaca gtccgcgagc caaagcgccg tttatcgcgc tcaatatggc
     6181 ggctatcccg aaggatttga tcgaatccga actgtttggt cacgagaaag gcgcatttac
     6241 cggcgcgaat accattcgtc aggggcgttt tgaacaggct gatggcggta cattatttct
     6301 cgatgaaatt ggcgatatgc cgctggatgt gcagacgcgt ttgctgcgcg tgctggcaga
     6361 cggtcagttt tatcgcgttg gcggctatgc gccggtgaaa gtagatgtgc ggattatcgc
     6421 tgccactcac cagaatcttg aacagcgggt gcaggaaggc aagttccgtg aggatctgtt
     6481 ccaccgcctg aacgtcatcc gcgttcatct gccgccactg cgcgaacgtc gggaagatat
     6541 tccccgtctg gcgcgccatt ttttacaggt tgccgcgcga gagctgggcg tagaagcgaa
     6601 gttgctgcat ccggaaaccg aagccgcgct gacgcgtctg acgtggccag gcaacgtgcg
     6661 ccagctggaa aacacctgcc gctggctaac ggtaatggct gccggacagg aagtgttgat
     6721 tcaggatttg cctggcgaac tgtttgaatc caccgtagca gaaagccctt cgcacatgca
     6781 gccggacagt tgggcaacgc tgttagcaca gtgggcagac agagcgctgc gttccggtca
     6841 tcaaaacctg ctttccgaag cgcagcctga gctggagcgg acgttactga ccaccgcgtt
     6901 gcggcatacg caggggcata aacaggaagc ggcgcggcta cttggctggg gccgcaacac
     6961 cctgacgcgt aagttaaaag agctggggat ggagtgattc acggcttgtg tgtaaagatt
     7021 gattattgag cgcaaattgc tggtattttg cgctttactg ttccgataag ttcagtatga
     7081 tcttgcccgg aaaacgggga gagtcattat gctggaatca ataattaatc tggtatcgag
     7141 tggcgcagtt gacagccaca caccgcaaac tgctgttgct gccgtgctgt gtgccgcgat
     7201 gattggtctg tttagctgaa gctcgttgtt tacgccggat gcggagaaag tcgtaggcct
     7261 gataagacgc aaccagcgtc gcatcaggcg ttgattgccg gatgcggcgt aaacgcctta
     7321 tccggcctac aaaagcaata atttcaacag attttgcagg cctgataagc gtagcgcatc
     7381 aggcaatctt gttctcagca cattgatggc gctttcgttt tctactttgt aaacgaagcg
     7441 ccattcacaa tttaaatcac ccgagagaac tgctgcatcc gcgctttctg gcgcagatag
     7501 gtatcaaagc acatgcaaat gttgcggatc agcaagcgac ctttcgccgt cacctgtatt
     7561 cccttctcat ccacatccac cagcccatct tttgctaacg gggcgagcag cttgagatct
     7621 tccgcaaagt aatcagcgaa gagcaaatcc cactgttgct caataggggc gtaatccaga
     7681 cggaagttgc agatgagcga cttaatcaca tcgcggcgaa tacagtcatc acgcgttagc
     7741 gcaataccac gccacagcgc attgccttgt tcatccactt gctgatagta ctgcttcaac
     7801 tctttctggt tctgcgcgta gcagtcgcca atcatgctga tggcggaaac gcccatcccc
     7861 agcagatcgg tatcgccctg agtggtgtag ccctggaagt tacgatgcag cacgccttca
     7921 cgctgggcca ccgccagctc gtcatccgga cgggcaaagt gatccatacc gataaactga
     7981 tagcccgatt gtgtcaggaa ggcgatggtt tcctgcagga tatcgagttt ttgctgcgga
     8041 ctcggcaggt cagcgtcttt gattttgcgc tgagcagcaa aaatggtcgg caaatgcgcg
     8101 tagttaaaga cgctcagacg atcggggttc agctccgcca cgcgtttcag ggtaaaggcg
     8161 aaactctccg gcgtctgttt cggcaggccg taaatcaggt cgatgttggt ggaggtaaag
     8221 ccaatctcac gcgcatggtt aagcagtgca aagatgaact cttcatcctg ctcgcggtta
     8281 accagacgtt gcacttcttt gttgaagtcc tgcacgccca tgctcagccg attaaagcct
     8341 tcggcgcgta aatgatcgag tacatccagt tcgatttccc gcggatcgac ttcgatcgag
     8401 atctccgcat cggcattgaa ctggaagttt tcgcgcagca gcttcatcag acggctgatt
     8461 tgcgctttgt tcagatacgt cggcgtacca ccgccccagt gcagctggct caccttgcgc
     8521 ccggcaaaaa gcggtgcacg atggacgatt tcttgctcca gcgcgtccag atactgatcg
     8581 gccttgtgct gctggcgagt aactatctta ttgcaaccgc agaagtaaca aagcttatgg
     8641 cagaacggaa tatgtacgta gagagataat ggacgctcag gatagcgcgc cacggcttgt
     8701 aaaaacgcct gttcgccgaa gtcttctgaa aactccagcg cggtcgggta cgaggtgtat
     8761 cgtggcccgg aatagttata tttctggatc agggccagat cccagtcgat ttgctgtaca
     8821 gacatgctca ctccttccga tggcgtctct ggcggctacg gcgtaccggc ggatgcccgt
     8881 ttcgggtcat cagccggttg cgcagccact tttgtcgccg cgacaaccgc tgtagtttaa
     8941 cgaataacca caccagataa catataaccg gaagggttat cagcagaacg ggaaggccca
     9001 cggcggcaat ccgttagttg ccccgcagca gacgcatcat gtcttcttgc ttctcgtctt
     9061 cttcctcttc ttcgtcatcg tcataagaga ggccgagttt ctgcatcaac tcatcaatac
     9121 gatccagttt agcatccacc caggattgct cttcggcgct cagggtttcg cctgcttcca
     9181 gacgttccag cagcgcatcc agacgctcat ccgtttccag taactccaac tccgcctgcg
     9241 gtgaaagcat aggtttctca ctcttcggtt tgtgctgttt ggtgactttt tcagtcacgc
     9301 ccaatggaat aggggtttta ctgccaatac gtggatcttt tggtgcgttc tgacctttgc
     9361 tgcctgacgt ggtgttaccg cccgctgcgc ggctgcccgg cgcatgacca cgacgttttt
     9421 tctgacgctt acggtcacga gcttcctgat ccagctcctc gcgtgttttt cgacgcgctt
     9481 ttgcatgacc tttgctgcgt gagtttgaag atgatggttt cataatggtt cgtcttttag
     9541 ccgttttatt cagtatagat ttgcggcgga atctaacaga aagcaagcaa agaaaaaagg
     9601 cgacagatta ctctgtcgcc ttttttcctg actcataacc cttaacgggt cttacaatcc
     9661 ttgcaggccg ccaacacacc ctgtttttcc attagccgga acaatccgtg ttgattccat
     9721 ttccatttaa ttacgtctcc ggacgtttat cttcctgaca atcctctgtc ttcgcctcct
     9781 ggcgctcctg accctcatcc tgagtcattg ttcctgttag cgtcctcgct ctatgggtgc
     9841 tactttacgc ctttgcttta aataaacaat caaccgccat cagtatttgc acattttcag
     9901 agttttatat aaaagcaatt cactgattta tatagcttat ttgacatgcg tcgcagaatt
     9961 ttctctgcta tttcgcataa agactatcgg caatgtctca caaagcatat ggcttttact
    10021 tacaacgctc acgccgaaaa atccgccttt tattatggtt caggtataat cgccgcaaag
    10081 ctttgactga tggagacgac cgctttgact aatttgaatt atcaacagac gcattttgtg
    10141 atgagtgcgc ctgatattcg ccacctacct tccgataccg gaattgaagt ggcttttgca
    10201 ggccgttcca acgcaggtaa atccagcgcg ctgaacacgc tgactaacca gaaaagcctg
    10261 gctcgtacct caaaaacccc agggcgcacc cagcttatca acctgtttga agtggctgac
    10321 ggcaagcgtc tggttgactt gcctgggtac ggttatgcgg aagtcccgga agagatgaag
    10381 cgcaaatggc agcgcgcgct cggcgagtac ctcgaaaaac gtcagagcct gcaaggtctg
    10441 gtggtgctaa tggatattcg ccatccgctg aaagatttgg atcagcagat gattgagtgg
    10501 gcggtagaca gcaatatcgc cgtgctggta ctgctgacca aagcggacaa actggcaagc
    10561 ggcgcacgta aagcgcaatt gaatatggtg cgtgaagcgg tgctggcgtt taacggtgat
    10621 gtgcaggttg aaacgttttc ttcgctgaag aaacaaggcg tggacaagct gcggcagaaa
    10681 ctggatacct ggtttagcga gatgcagcct gtagaagaaa cgcaggacgg cgaataattt
    10741 tcttgcctta atgcttgtgc cggatgtggc gtatccggcc cgtaaattca atagattaca
    10801 attctaccgt tggcctgata aacatggcgt atcaggcatt acggatcttt tctttcgccc
    10861 aataaaaaac gccccagtca ttactgactg gggcggctaa aatattcagc caaatccgat
    10921 tacgtgaagt aaaaggtctg aaagatagaa catcttacct ctgtacccta cgcgactaac
    10981 tttactcttt tttgttcagt tcagaaagca ttttttgtaa ttttttttca gttgttgcat
    11041 aggaatttct aatagccatc acaaaacgtg acagcttatg ttgcttactt acgaaaaaag
    11101 ggcatgttca ggcgaatctt agtgcgcctg atcccagttt tcgccactcc ccacttccac
    11161 cagtaacggc acatccagac gggtacagtt ttccatcagt tgatgaatct gcttcgcgac
    11221 ggcatcgaca tcatctttat ggacttcaaa taccagttca tcgtgtacct gcatgatcat
    11281 acgcacacgc ggttgctcag cctgtagcca cgcatcaacg gcaatcatcg cccgtttgat
    11341 aatgtcggcg gcggttccct gcattggcgc gttaatggct gcacgttcag ccgctgcacg
    11401 acgagcacca ttgctggatt tgatatccgg cagatacaaa cggcgtccgt ccagcgtttc
    11461 aacgtagccc tgctctttcg cctgagcacg ggtgcgttcc atatactcca gcacgccagg
    11521 gtagcgttcg aagtaaaggt ccatgtactt ctgcgcttct ttacgcggaa tgttcaattg
    11581 ccgcgccaga ccgaaagcac tcatgccata aatcagacca aagttgatcg cttttgcact
    11641 acgacgttgc tcgctggtga cggtttccag tggcaaacca aacacttctg ccgccgttgc
    11701 acggtggata tcttttcctt cggcgaatgc ggtcagcaag cctttgtcac gcgagagatg
    11761 cgccataatg cgcagttcaa tctgcgagta gtccgctgag acaatcacat aatcctctgg
    11821 cgcaataaac gcctggcgga tacggcgacc ttcttcgtta cgcaccggaa tgttttgcag
    11881 gttaggatcg gttgacgata aacgtcccgt tgcggttact gcctggtgat aagaggtatg
    11941 tacacgcccg gttttcgggt tgatcatcaa cggcagcttg tcggtgtagg tcgatttcag
    12001 cttcgccaga ccacgatact ccagaatcac ttttggcaac ggatagtcca acgccagttc
    12061 ttccagtacc tcttccgacg ttgacggcgc gccacccgga gttttcttca gcggtttaat
    12121 gccctgtttt tcaaacagaa tggtttgtaa ctgcttggtg gaagaaaggt taaattcttc
    12181 acctgcaatt tcatgcgctt tcttttccag ctcagccaga cgcaaggtga gctcttcaga
    12241 gtgattgtgc agcactttcg gatcgatctt cacaccgtta cgttcaatgc gtgaaagcac
    12301 cggcaccagc ggcatttcga tattctcgaa gacgttcaac ggccctttgt gtttttgcag
    12361 atccggccac attttcagat gcaactgcaa ggtgacatct gcatcttcgg cggcgtaacg
    12421 tccggcttct tcgagggcaa tctggttaaa ggtcagttga tttttgcctt tacccgcaat
    12481 ctcttcaaaa gtgatagttt tgtgcttcaa ccaacgttcc gcgaggctgt ccatatcgtg
    12541 acgtccggca acgctattga gaatgtagga ctccagcatg gtatcaaacg caatcccacg
    12601 tagttcaatg ccgtagttcg ccagaatacc gcgatcgtat ttcaggtttt gcccgacctt
    12661 cagcgccttt tcatcttcca gcagcggttt tagcagctcg agtgcacgct cgcgagagat
    12721 ttgatcgggc gcatcaagat aatcatgagc aaccggaata tatgccgcta cgcctggctc
    12781 gatagcaaaa gaaagcccga ccaggttagc agagatgtta tcaaggctgt cagtttcggt
    12841 atcaaatgca aataccggcg ctttttccag cttcgcaatc cactctttca gtgtttcttc
    12901 atcaaggatg gtgacgtagt tgtcataaga aatcaccgtt gccgtcactt ctggcgcttc
    12961 gtctgcaaca ctggtttcct gcggcctggc tgcgggtttc gcccctttag cctgtagcca
    13021 ttttcccgct tcgacatcag cagtccagcg tttgaactca tactttttga acagccctaa
    13081 caactcttcc gccgccggtg gctgcacttc cagttgttca caggtcagct ccagttcaac
    13141 gtcggtttta atcgtcgcca gctggtatga gagataagca acttctttgt tttgctcgag
    13201 cttcgctgcc attgttttcg cgccacggaa gctcaaccca gcaatttttt ctggctcggc
    13261 atacagcgta tccagcccgc caagaccttg cagcaatgcc tgcgcggttt tttcaccgac
    13321 gcccggtacg ccaggaatgt tatcagagga gtcacccatc agcgccagga aatcgatgat
    13381 cagttctggc ggcacgccgt acttattcac cacctcttcc ggtccgagga tggtattcgt
    13441 catggtgttg ataagcgtga tatttggtgt caccagctgc gccatatctt tatcgccagt
    13501 gctgatcagc accggacgcc ctgctttttc ggcttcgcgc gccagagtac caataacgtc
    13561 gtccgcttct acgccagaaa ccgccagcag cggcagtccc atcgctttaa ccatcgcgtg
    13621 cagaggttcg atttgtgctc gcagatcgtc cggcattggc gggcgatgtg atttgtaatg
    13681 ttcaaacagt tcatcacgaa aggtttttcc cttggcgtca aagaccaccg ctgcatgcgt
    13741 cggtttatat tgcatgatca gactgcgcag catgttgagg acaccataca tcgcaccggt
    13801 cggctcgcct gcgctgttag tcagcggggg aaacgcgtga tatgcgcgat aaagataaga
    13861 tgaaccatct acaaggataa gtggattttg ggggatctga accataatgt ccgtgcctgt
    13921 ttatcagatt atgggtaaag gatgccacag acaggatgaa aacatgagtt tttcaccgct
    13981 tttcgtgaaa atcttttgta gatcttctgg atcgctcgca aatcgtcatg tggataactt
    14041 tgtgcataaa ttatttacat cgcttttaat ttattatcat gctttttaca gcatggtgtt
    14101 tgtctttgtt tttcatgcag ttaaaaataa agccatccct aataattgag ataatgggat
    14161 tttttctaat gtggatagat atgaattatt tttctcctta aggatcatcc gttatttggg
    14221 tcgttttctg ctaaaatctg cgccttgcgc ttctttagcg cactgtctat ggctaacctt
    14281 ttgaataaat tcattatgac gagaatactc gctgcgataa cccttttgct gagtatcgta
    14341 ttgaccattt tggtcactat tttttgttcc gtcccgatca tcattgccgg gattgtaaaa
    14401 ctattgctac ctgtgccagt catttggcga aaggtttcgc gtttttgtga ttttatgatg
    14461 tattgctggt gtgaaggtct ggcggtatta ctgcatctga acccacacct gcaatgggaa
    14521 gttcacgggc tggaagggtt aagtaagaag aactggtatc tgcttatttg taaccaccgt
    14581 agttgggcag atattgtcgt actgtgcgtg ctgtttcgta agcacattcc gatgaataaa
    14641 tatttcctca agcagcagct ggcctgggtg ccttttcttg gcctggcgtg ctgggcgctg
    14701 gatatgccgt ttatgaagcg ttattcccgc gcttatttat tacgtcatcc ggagcgacgt
    14761 ggtaaagatg ttgaaaccac tcggcgttct tgtgaaaagt ttcgcctgca ccccaccact
    14821 attgtcaatt tcgttgaagg ctcccgtttc acgcaagaaa aacatcagca aacccactcc
    14881 tcttttcaaa acttgttgcc accaaaggcg gcaggcattg cgatggcgct aaatgtactg
    14941 ggtaaacaat tcgataaact gcttaatgtt acgctgtgtt atccggacaa taaccgtcag
    15001 ccgttcttcg atatgttaag cggtaaactg acgcgaattg tcgtacatgt ggatttacag
    15061 cctattgccg atgagttaca tggcgactac atcaatgaca aaagctttaa gcgccatttt
    15121 cagcaatggc tgaactcatt gtggcaggaa aaagaccggc ttctgacatc gttgatgtca
    15181 tcacagcggc aggaaaagta agttttactc gcctctgaat gagcagaggc gagtgagtat
    15241 tttaatgaac aaaacgtccg gcacgagaca taaattcttc ttcgctcatc tcctgtccgt
    15301 taaaaacaac tttacccggt gtataacgca gttgcagcga ggcggtattg ttgtcaatcg
    15361 tgattaactg taacatcaga cctaatgtca tcatcccact gatttgctta tcagcccgct
    15421 tttgcgcttt ttccgcatcc atcccttcag ataaattaag ctgttttgcg gtttctgtgg
    15481 cgacattaag cggtaacttc atatcaaaat tgagcgattt aatatctttg tttgtggatg
    15541 atgaagattt ggctgggtcg gcaatactga tatccagatt ggcatttagt tcgccgagtg
    15601 cgttcttcca tcttactggt tgtttaatgg tcggctcact tttttgtaat aacggtaaat
    15661 actctttgaa caatgcagca ttcacttctt gcagagcaac ttcatcgttt gctaactcag
    15721 ggtgtgcagc aagctgcttc tgcatcgcaa tgttatactg gatgataaat tggcgcaccg
    15781 ctgagggatc aatagactca gcaatcaatg accactcacc actaccgagt tgctgattcc
    15841 cttttgttaa cttatcaagt tcgtaggtca gattgacatt gatataatct ttgtctttaa
    15901 cgcgatccag cctggttttt gcaacgaaag catcgatttg ggcaacgact tccccctggt
    15961 tgataatatt aacctgatta aatttcgact cgctttcacc aaccggaaat ttcttttctt
    16021 ctaatcgtgt cagagaatca atggtaaaac ttttcgccgt tactttgttc attggtgaaa
    16081 attgcagaac aatatcttcg actttgcctt caatatccag gttagacaat gaactatcat
    16141 caccattaaa agtaaactgg ccttcaccaa aagaaaactt tccatactct gcaggggcaa
    16201 catctaatgt cgtggagaat gggccaccaa acgcgaatcg caatgtcgct tccacgggcg
    16261 atttattttt cgcggcgata aatagcggtt gtgtcagctc attattcact aagttcactt
    16321 ttgccgctgc cagtgctggg atgacattac catgcattaa cattgtgatg ggcaacggac
    16381 cgtgctcaac atccacatca aatacaattt tctggcctgg cttgatattg agatcgggtg
    16441 caccgttatc aaatgtcatt tgcatttgaa aacgggaatt aaaaaagcct ttgtcaaaat
    16501 ttttataact taacgtcata tcgtaggcat gttcaccttt ctttttagca tcgttaaaac
    16561 ctttaataaa cttttcgaca ccaggctgaa tttgcgtacc tgtgtaccat gtgccaccac
    16621 cccagattac ggctaacgca acaatggcac ctgtagctga cttacgtatc atataaatat
    16681 tccctgataa aacagcaaaa ctttgaatat cc
//
LOCUS       DADSZD010000098        16379 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.099, whole genome shotgun sequence.
ACCESSION   DADSZD010000098 DADSZD010000000
VERSION     DADSZD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.099"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(69..887)
                     /gene="cof"
                     /locus_tag="J1Z28_002889"
     CDS             complement(69..887)
                     /gene="cof"
                     /locus_tag="J1Z28_002889"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBA7717706.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            987..2687
                     /locus_tag="J1Z28_002890"
     CDS             987..2687
                     /locus_tag="J1Z28_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBA7717707.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            2752..3447
                     /gene="queC"
                     /locus_tag="J1Z28_002891"
     CDS             2752..3447
                     /gene="queC"
                     /locus_tag="J1Z28_002891"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBA7717708.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(3499..3897)
                     /locus_tag="J1Z28_002892"
     CDS             complement(3499..3897)
                     /locus_tag="J1Z28_002892"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HBA7717709.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4003..4374)
                     /locus_tag="J1Z28_002893"
     CDS             complement(4003..4374)
                     /locus_tag="J1Z28_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717710.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(4525..6396)
                     /gene="ppiD"
                     /locus_tag="J1Z28_002894"
     CDS             complement(4525..6396)
                     /gene="ppiD"
                     /locus_tag="J1Z28_002894"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBA7717711.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEITRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(6588..6860)
                     /gene="hupB"
                     /locus_tag="J1Z28_002895"
     CDS             complement(6588..6860)
                     /gene="hupB"
                     /locus_tag="J1Z28_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBA7717712.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(7069..9423)
                     /gene="lon"
                     /locus_tag="J1Z28_002896"
     CDS             complement(7069..9423)
                     /gene="lon"
                     /locus_tag="J1Z28_002896"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBA7717713.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(9611..10885)
                     /gene="clpX"
                     /locus_tag="J1Z28_002897"
     CDS             complement(9611..10885)
                     /gene="clpX"
                     /locus_tag="J1Z28_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBA7717714.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(11011..11634)
                     /gene="clpP"
                     /locus_tag="J1Z28_002898"
     CDS             complement(11011..11634)
                     /gene="clpP"
                     /locus_tag="J1Z28_002898"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBA7717715.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(11880..13178)
                     /gene="tig"
                     /locus_tag="J1Z28_002899"
     CDS             complement(11880..13178)
                     /gene="tig"
                     /locus_tag="J1Z28_002899"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBA7717716.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(13522..13839)
                     /gene="bolA"
                     /locus_tag="J1Z28_002900"
     CDS             complement(13522..13839)
                     /gene="bolA"
                     /locus_tag="J1Z28_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBA7717717.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            14144..14722
                     /locus_tag="J1Z28_002901"
     CDS             14144..14722
                     /locus_tag="J1Z28_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7717718.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            14766..16241
                     /gene="ampG"
                     /locus_tag="J1Z28_002902"
     CDS             14766..16241
                     /gene="ampG"
                     /locus_tag="J1Z28_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBA7717719.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNGNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         3749 a         4395 c         3929 g         4306 t
ORIGIN      
        1 cacggcacaa aatgacaaat tcaacgcaaa aaaaaccggg caattgcccg gtgctggaag
       61 ggatctcgtt attcggggga ataaggtaga tgtggatagt ccagccagtg cgtcaaatag
      121 tgagagacag cctgatttcg gcaatgtcca atcaccggta aatgcgggag ctccgcgcgc
      181 agttgcggca tcgcattgcc cataataaat ccgctaccga cactgcctaa catttcgcga
      241 tcgttcatcg catcaccaaa ggccatgcaa tcgcgtaacg ataaacctaa atgttgggtc
      301 agcaccgtca atgcagcgcc tttattgcag cccaccggca gcacttcgag gcaatccgtg
      361 gcggaaaaac ataaatgtgc acgctcgcct aatgcttcgt atagctggat ctgcaagcgt
      421 gtaagatcgt cgtgatcgcc acagaagcag atcttggtga cgctgccgag tggcattttt
      481 ttgacatcga ttatctgata acgaaaaccg ctatagacaa atgcctgcaa caacgcaggg
      541 atctctttcc cggtaaacca accatcgtca ttgaagatat gcatgcttgc tcgggtatcc
      601 cattgctgat acagcaccag ctccgcgaca tccgcaggta aatcatcacg atgtaaaagt
      661 tcaccttcca gagaatgcac gcgcgttccg ttgccggtaa tcaaatatgc atccagcgat
      721 agcgccccga gaatatgctg catctccagc gcatgacgcc ccgtggcaaa agtgagggta
      781 atgtcgcgtt cacgcagccg cgccaaagta gagagggttt tctcacctaa atgatggtca
      841 ggcatcaata aagtgccatc catatcaaat gctgccagac gagccatttc tttctccact
      901 ctgtgacacg gttaatggtg attgagtatc acctgatata tacggaagta atagtgaata
      961 gttaaataat attgttccgg gtttatatgc gactgctcaa ccgtcttaac cagtatcaac
     1021 gtctgtggca accttccgcc ggaaagccgc aaaccgtcac cgtcagcgaa ctggccgaac
     1081 gctgtttttg cagcgaacgc catgttcgta cgctgttacg ccaggcacag gaggcgggat
     1141 ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg tcaactacgc tttctggtca
     1201 cgccagaatc gctacgcaat gcgatgatgg aacaggcgct ggaaaccgga aagcagcaag
     1261 atgtgctgga gctggcgcaa ctggccccag gtgagttgcg cactctgtta cagccgttta
     1321 tgggcggaca atggcaaaac gatacgccca cgttgcgtat tccctactat cgcccgctcg
     1381 aaccgctaca accgggcttt ttgcccggcc gtgccgagca gcatctggcc gggcagatat
     1441 tttccggcct gacccgcttc gataataata ctcagcgccc gattggcgat ttagcacatc
     1501 actgggaaac ctctactgac gggttacgct gggactttta tcttcgttca acgctacact
     1561 ggcataacgg cgatgcagta aaagcctcac acttacacca gcgattattg atgctgttac
     1621 aactgccagc actggatcaa ttatttatta gcgtgaagcg tattgaagtc acccatccgc
     1681 agtgtctgac cttcttttta catcgccccg attactggct tgcgcaccga ctggcgagct
     1741 attgcagcca tctggcgcat ccgcaattcc cactgatcgg cactggtcct tttcgcttaa
     1801 cacaattcac agcggagctg gtgcgcctgg aaagccatga ttattaccat ttacgtcatc
     1861 cgctgcttaa agcggttgag tactggataa ctccgccgct tttcgaaaaa gatttgggaa
     1921 ccagttgtcg gcatcccgtg caaatcacca ttggcaaacc ggaggagctg caacgggtca
     1981 gccaggtcag tagcggcatc agtttaggtt tttgctattt aacattgcgc aaaagtctcc
     2041 gactgtccct ctggcaggcg cgaaaagtaa tctccattat tcatcaatcc ggtttattac
     2101 aaacgttaga agtcggagaa aacctgatca ccgccagtca tgcattactg ccaggctgga
     2161 ctattccgca ttggcaggta ccggatgaag ttaaactacc gaaaaccttg acgctggttt
     2221 atcacctacc gatagaactt cataccatgg cagaacgcct acaggcgaca ctggcagcgg
     2281 aaggttgtga acttacaatt atttttcata acgcaaaaaa ctgggacgat acgaccctac
     2341 tggcacacgc agacctcatg atgggcgaca gattaattgg cgaagcaccg gaatataccc
     2401 tggagcaatg gctgcgttgc gatccactgt ggccacatgt tttcgacgct ccagcatatg
     2461 cacatttgca atcgacactg gacgcggtgc aagtaatgcc tgatgaggaa aaccgattta
     2521 atgccctgaa agcggttttt agccagttaa tggcagatgc gacgctgaca ccgctgttca
     2581 actatcacta tcgcattagc gcccctcccg gcgtgaacgg tgtgcgactg acaccgcgcg
     2641 gctggtttga atttaccgaa gcctggcttc ccgcgccgtc gcaatgaata gctggtccgg
     2701 gttaatcgca ggcgttacca taacgctttt attgttcaag caggattatc tatgaaacgt
     2761 gctgtcgttg tgttcagtgg aggccaggat tccaccacct gtctggtgca ggcattacaa
     2821 caatatgatg aagtccattg cgtgacgttc gattacggtc aacgccatcg cgcagaaatc
     2881 gacgtggcac gcgaactggc gctgaaactg ggggcacgcg cgcataaggt gcttgatgta
     2941 acgctgctca acgagctggc ggtcagcagc ctgacgcgtg acagcattcc ggtgcctgat
     3001 tatgaacctg aagccgatgg tatcccgaat acgtttgtcc cagggcgtaa tattttgttc
     3061 ctgacgctgg cggcaatata tgcgtatcag gtaaaagcag aagccgtaat taccggcgtc
     3121 tgcgaaacgg atttctccgg ctacccggat tgccgcgatg agtttgtgaa agcactaaac
     3181 catgccgtca gtttgggcat ggcgaaagat attcgttttg aaacgccgct gatgtggatt
     3241 gataaagcgg aaacctgggc gctggcagat tattacggca agctggattt agtccgtaac
     3301 gaaacgctga cctgttataa cggcattaaa ggcgacggtt gcggtcattg tgcggcgtgt
     3361 aatttacgtg ccaacggttt gaatcattat ctggccgata aaccgacggt gatggcagcg
     3421 atgaagcaga aaaccgggtt gaagtaatta ttccgggtgt cgccggatgc ggcttgagca
     3481 tccggcacca caaaacgttt acttaaccat ctgctccagc ttttcgcgca attccccttc
     3541 cagagctaat gctttctgcg ttttaagatc aatacaaaca aacgtaataa gcgcatccgc
     3601 taccacctgt ccttccggct ccagtgtaat gacctggctt aagatgccac ttttaccgtt
     3661 taattgctgc aactgactgg taatggttaa caggtcgctt aataccgccg ggcggcgata
     3721 gttaatattg atattgacca cgacgaaggc gatgttatgg gccgtcatcc agtgaaaact
     3781 gtcgctattt tccaacccat gccagcgggc ttcttcgaga aattcaaggt agcgggcgtt
     3841 gtttacgtgc tggtaaacgt cgagatgata tccacgaact ttgatttgtg tttgcatagc
     3901 gcaataacct tacgttgttg ttataagaac tgttataagt acagaggtca taactggtat
     3961 gacctcttca gtctggcaaa aaattgccac tatgcaaatt aattacaggg ttaataccgc
     4021 cagattacgt tccaccagcg aattgcccat ccccggcacc tgctttagat cctccaccgt
     4081 tttaaacgga ccgtactctt cgcgataact gacaatcgcc tgcgctttct tcaggccaac
     4141 gccattcatc gcgcgggcta actcttccgc gctggcatta ttaatgctga cccgggtgcc
     4201 ttcttcgtca ctggctttcg ccggtactgc tgctttactt tgtgctgcag gagcttccgc
     4261 tttggtttct actgttgtcg gtttcgccac agaagctgcc gccagcgcgc tatgagacat
     4321 tccggcacag gccagggaca gggtaatgag cagtgcttta attccgtgtt tcatactgtt
     4381 ttctccttgt ttgttaacag tggaatcacc ttaacggcag tgaaaacagt aaacaaatgg
     4441 caatgttcag aaatggaaaa ggccgcgaaa gcgacctttt cttgttacag ctgcaaagca
     4501 tttttttgcg aggcggcttc aggattattg ctgttccagc gcatcgccaa ttttgatttt
     4561 cgcctcttta cgcaggttac tcatcagagc ttcaaagacg atttgtgcgt tgttctgggt
     4621 gataccctgc accatcgctt ttttctgatc ttccggcatt gaaccttgtt tcacttcatc
     4681 cagcgccagc agaaccacat taccttgcat atcggtcgcc ataccgtagc tcggtttgtc
     4741 tttcgctggc agtggcagtg caaacgcagc ctggctaatc gggtcacgac cggaacggct
     4801 taaggttttc ggctcgccaa atttcagacc ggcagcctgc atagcttccg cacctttgcc
     4861 ggctttcaaa tcaaccagca gtttctcagc atccactttc gcctgttgtt cagctttgtt
     4921 gtgctgaacc agtgccttaa cttgttcctg aacatctgcc aacggtttca ccgcttccgg
     4981 tttgtgctcg ctgatgcgca gcacgaatgc gcggtcgccg tctacggtga tgatgtcaga
     5041 gttgataccc ggcgcgccgt tttcacctac cagaccgccg ttgaagatag cgtcggcaac
     5101 tggcttgaag ttcaactctt ccggcaggtt atctttgctg aaccaacccg tctgagtggc
     5161 tttaacgccg gcagcttgct ctgcaccggc cagagactcg gtgtcgttgc ttgccgcatc
     5221 gctcactttc tgctgcagcg cgtagtacgc atcgagggct ttttcgtgtt tcactttcgc
     5281 cgcaatgtcg tcacgtactt cgtctaacga tttcactttc gctggctgaa tgtcgtccag
     5341 acgcacaatc aggaaaccga ccgaagattt gataacacca gacagttggc ctttttcttt
     5401 cagacccgca tttttcagtt cgtccgggat agtggcatct tctaaccaac ccatatcacc
     5461 gccgttgcgg gcagagataa tatcggcaga tttttctttg gctaatgcag caaaatcacc
     5521 gcccttattc agctcatcaa gtaccgcttt cgcttcatct tcagttttgg tctggatgat
     5581 gctgtaacgg gtacgctgcg gctgggtgaa ttgatcctga tgctgatcgt agtagctctg
     5641 gatatccgca tcgctaaccg gttgctgcat cgtcgcggca tccagcttga tgtaactcac
     5701 gcggaattgt tccggcgtca tgaaattgtt tttgttttgt tcgtagtagc tggcaatttc
     5761 ctgttcggtc acaggctgct tcgccgccag cgcgttaaca tcgatagtcg cttcacgcac
     5821 cacgcgttgt tgagctacca gtgccgccag ctcgtcggtt tcacctttca gcataaaatc
     5881 ggtaccggca acgccgttaa tcagctgttg ggttgtgagc tggttacgca gcgcttgggc
     5941 gtactgatcg gcggtcatcc ccatctggtt gaggataccg ttatagcggc tgttatcaaa
     6001 tttgccgtca acctggaagg ctggggtcgc gaaaatcgcc tgtttaacct gctcatcgct
     6061 gatacccagt ttcagctcac gagcgtactg atccagaagc gcctcgtcga tcagacgatt
     6121 cagcacctgt tgacgcaggg ttttcatata gccttcgttc gctgccagct cggagtattg
     6181 atcgcccagc tgttgctgca tgcgattacg ctcgctgttg aaggcgttct cgaattgccc
     6241 acgggtgatt tcctggtcat tcacttttgc ggcgtagtta ttgcctccgc caatcaggta
     6301 accactcacg ccggtcaata tgaacgacac gataatgata ccgaaaataa tcttgagcac
     6361 gagactgttt gcagccgtgc gtaagctgtc catcatggtg taacaacact ccgctgtaga
     6421 tgactgtgta cctcacgcaa cgtatcgatg cgcgcaaagg cctattgtga caagaaacgg
     6481 gggcaattgt cagcccacaa gacgcagaaa gtcaccgaat acaaataaaa aaggcacatc
     6541 agtagatgcg cccttgaact tcgtcacatc cccactgggg acaacgctta gtttaccgcg
     6601 tctttcagtg ctttacctgc acggaagctc ggtactttag cagcagcgat ggtgatttct
     6661 ttaccggtct gcgggttgcg gccagtacgg gcagcacgct ctttaacggc aaaagtacca
     6721 aaacctacca gtgctacatc atccccttct ttcagagatt cagttacgga agcaataata
     6781 gcatctaacg cacggccagc cgcagcttta gagatatcag cccctgcagc aatcttgtcg
     6841 atcaattgag atttattcac tcttctcttc ctctttataa tttatatcgc acttgaatcc
     6901 ttcaaggtac gaacgcgcag cagttatatc aggccagcca tccccttaca acacccctta
     6961 ataagggcaa gcctgatccg ccatctaact tagcgagaca aaaaaaggct ggcaagcccg
     7021 aattagcctg ccagccctgt ttttattagt gcattttgcg cgaggtcact attttgcagt
     7081 cacaacctgc atgccagacg gttcattttg tagcgccaga gtcagaactt cctcaatgcg
     7141 cttcacagga tgaatgtcca gatcggcaat tacgttgtca ggaatctctt ccagatcgcg
     7201 tttattttcg aacggaatta acactgtttt aatcccgccg cgatgcgctg ccagtagttt
     7261 ttctttcaaa ccaccgatcg gcagtacctg accacgcaga gtgatctcac cggtcattgc
     7321 cacatcggca cgaaccgggt taccggtcag gcaagaaacc agcgcggtgc acatggcaat
     7381 accggcactc ggaccatctt tcggcgtcgc accttccggt acgtggacgt ggatatcacg
     7441 tttttcgtaa aaatcagggt tgatccccag tttttccgca cgcgcacgaa ccactgttaa
     7501 cgccgcctga atagactcct gcatcacttc gccgagcgaa ccggtatagg tcagtttacc
     7561 tttccccgga acgcacgcgg tttcaatggt cagcaagtca ccgcccactt ccgtccacgc
     7621 caggccggtt acctggccga cacggttttc gttatccgcg cgcccatagt cgaaacgctg
     7681 aacaccgagg tagtcatgca ggttatcgcc gttaatttcg atatgtttta atgacttatc
     7741 gagcagtaac tgcttaaccg ctttgcgaca cagtttggag atttcacgct ccagaccacg
     7801 cacgcccgcc tcacgggtgt agtaacgaat aatgccgata atggcgctat cgtcgacggt
     7861 cagctcacct tttttcagtg cattacgttc aatctgcttc ggcagcaggt gacgtttggc
     7921 gatgttcagt ttttcatctt cggtataacc ggagaggcga atcacttcca tacgatccag
     7981 cagcggtgcc ggaatgttca tggagttcga cgtagcgaca aacattacgt cgctgagatc
     8041 gtaatccact tccaggtagt ggtcgctgaa cgctacgttc tgctctggat ccagcacttc
     8101 aagcagtgcg gaggccggat cgccacgcat gtcagaagac attttgtcga tctcatcgag
     8161 caggaacagc gggtttttca cgcccacttt cgccattttc tggatcagtt tacccggcat
     8221 agaaccgatg taagtacggc ggtgaccacg gatttccgct tcatcacgca cgccgcccag
     8281 cgccatacgg acatatttac gcccggtggc tttggcgatg gactgaccca gggaggtttt
     8341 acctaccccc ggcggcccta ccaggcagag gatcggtccc ttgattttgt tgacacggct
     8401 ttgaaccgca agatactcaa ggatacgatc tttcacgcgc tccagaccat aatggtcggt
     8461 atcaaggatt tcctgcgcct gacgcaggtc ttttttgacc ttgctacgcg cattccacgg
     8521 cacctgtacc atccagtcga tatatccacg cactacggtc gcttctgccg acatcggaga
     8581 catcattttc agcttctgca gctctgcttc cgctttctct tttgcctctt tcggcatctt
     8641 cgccgcgtcg attttgcgct tcagggcttc gttttcgtcc ggcgcgtcgt ccatttcacc
     8701 aagttctttc tgaatagctt tcatttgctc gttcagatag tactcacgct gggatttctc
     8761 catctgcttt ttaacgcggt tgcgaatgcg tttctcaacc tgcagcagat cgatttccga
     8821 ttccatcatt gccatcagat attccagacg ttcgttaacg tcggacatct ccagaacgga
     8881 ctgtttgtca gccagtttca gcggcatatg tgcagcaatg gtatccgcca gacgcgccgg
     8941 atcgtcgatg ctattcagcg acgtcagcac ttctggtggg atttttttgt ttagcttgat
     9001 gtagccttcg aactggctga ttgcagtacg caccagcact tcctgttccc gctcatcaat
     9061 ggtcggcgac tccagatact ccgccttcgc agaaaagtgt tcgccattgt cagagagcgc
     9121 agaaatacgc gcgcgctgta acccctcgac cagcactttg acggtgccgt caggcagttt
     9181 cagcatctgc aatatagagg ccacggtccc gacagtgaaa agatcgttta cacccggctc
     9241 atccgttgaa gcttctttct gcgcgaccag cataattttt ttatcatggt ccatcgccgc
     9301 ttccagacaa cggatggatt tttcccgccc gacaaataag gggatgacca tgtgcggata
     9361 aaccaccaca tcgcgcagcg gcaatacggg gatttcaatg cgttcagaac gctcaggatt
     9421 catagagctc tctcttagtt taatttccgc caggtaatca gatgacacga ctgtgcttca
     9481 cgccatctat taacatgtac gtcagtatat ggggatgttt cccccacatt caacgccgag
     9541 aatagaggaa aaattaaagg ggagataaaa tccccccttt ttggttaact aattgtatgg
     9601 gaatggttaa ttattcacca gatgcctgtt gcgcttccgg cttaccataa atcagcaacg
     9661 gtttgctttg accatcaatt accgactcgt cgataaccac tttttcgaca tcttccatgg
     9721 acggcagatc gtacatggta tcgagcagtg cggcttctac gatggaacgc agaccacggg
     9781 caccggtttt acgcgccatc gctttcttag cgatagcatc cagcgcctcg tcacggaatt
     9841 ccagatccac gccttccaga ttaaacagcg cctgatactg cttggtcagg gcgtttttcg
     9901 gctctttgag gatctgaatc agagcttctt cgctcagttc attcaacgtt gcgacaaccg
     9961 gcagacgacc aatgaactca gggatcagac caaacttgat cagatcttcc ggttcaacct
    10021 gcgccagcag ctcgccttcg cttgctttgt cggacttcgc ttttaccgtc gcgccaaaac
    10081 caatgccgga accggtttct acacgatggg aaatcacttt atccagaccg gcaaacgcac
    10141 cgccacagat aaacaggatc ttagaggtat caacctgcaa gaattcctgt tgcggatgtt
    10201 tacgcccacc ttgcggcgga acagcagcta ccgtaccttc gatcagtttc aacagtgcct
    10261 gctgtacgcc ttcaccggaa acgtctcggg taatggacgg gttgtctgac ttacgagaaa
    10321 tcttgtcgat ttcatcgatg tagacaatcc cgcgctgcgc tttctgtacg tcgtaatcgc
    10381 acttctgcaa cagcttctga atgatgtttt caacgtcttc gcccacataa ccggcttcgg
    10441 tcagcgtggt tgcgtcagcc atggtgaacg gaacgtccag caggcgcgcc agcgtttcgg
    10501 ccagtagcgt tttaccggaa ccggtcggac cgatcagcag aatgttactt ttgcccaact
    10561 cgacgccatt gctggtatct ccgttgcgca gacgtttgta gtggttgtat accgcgaccg
    10621 ccagcacttt tttcgcctgt tcctgaccga taacgtaatc gtccaggtgg ttgcgaattt
    10681 catgcggcgt cggtagcgca ctgcgttcac gatgcggtgc aacttcttta atctcttcgc
    10741 gaatgatgtc gttacataaa tcaacacatt cgtcgcagat atacacggat ggaccggcaa
    10801 tcagcttgcg cacttcatgc tggcttttgc cgcaaaaaga gcaatacagc aatttgcctg
    10861 agccatcttt gcgtttatct gtcatgggtc aaaacctctt ctttgttctt tgtgccgcac
    10921 acgacgacgc aaatgccatt ctcaggcgca agccgcttac agcgttgtgc cgccctggat
    10981 aagtatagcg gcacagttgc gcctctggca tcaattacga tgggtcagaa tcgaatcgac
    11041 cagaccgtat tccaccgctt caggggcaga aaggaagcga tcgcgctcgg tatcacgttc
    11101 aatctgttct aatgattgac ccgtatgaag cgccataagt tcattcatgc gccctttaac
    11161 tttcagaatt tcacgggcat gaatttcgat atcggtcgcc tggccctggt agccgcccaa
    11221 cggctggtga atcatcacgc gcgaattcgg caggcagaaa cgtttacctt ttgcccctgc
    11281 agtcagcaag aaagcaccca tcgaggccgc ctggcccata cagatggtgc tgacatcagg
    11341 cttgataaac tgcatggtgt catagataga catcccggca gtaatcaccc cgcctggaga
    11401 gttaatgtac agatagatat ctttttctgg gttttctgct tccaggaaca gcatctgcgc
    11461 cacaatcagg ttagccatgt gatcttcaac ctggccagtc agaaaaatga cgcgttcctt
    11521 aagtagacga gaatagatat caaaagagcg ctcacctcgt gaggtctgtt caatgaccat
    11581 cggcaccagc gccatatggg gtgcaaagtt atctcgttcg ccgctgtatg acatttccgt
    11641 ctcctggata aaattgaaaa aacctgctgt accgattgta accttatgga cggattatca
    11701 gctagtccct gtgttatggg tacggcatca ccatatttca agcataacaa tcttttgttg
    11761 ttacgctaac accgaaagag gcttttcgca cggttccatg caaaattcat gacaaaaaaa
    11821 agcccaccac ctggcggtga cgggcctttg tgcgaattta gcgcgttata ctacgtaaat
    11881 tacgcctgct ggttcatcag ctcgttgaaa gtggtttctt tttcagtcac tttcgctttc
    11941 gccagtacag cttcaacagc ctgttcttcc agagcaacat tgcgcatgtt gtccatcagt
    12001 tctttgtttt tgctgtagaa ctcgataact tctttcggat cttcgtacgc agaagccatc
    12061 tcttcgatca ggcctttcac gcgctcttcg tcagctttca gctcgttggt gcggataact
    12121 tcgcccagca gcaggccaac aactacgcgg cgtttagcct gttcttcgaa cagttcgcgc
    12181 ggcagttcca gagcttgttt ttcgttgcca ccgaaacgct gtgcagcctg gcgacgcaga
    12241 acgtcgattt cgctgtcgat cagcgcagcc ggtacgtcga tgtcgttagc ttttaccaaa
    12301 ccttcgatcg cctgagactt aacgcggtta cggatggcgc tcttcagctc gcgctccatg
    12361 tttttacgca cttcagcgcg cagaccttct acggaaccat cttcaacgcc gaaacgtttg
    12421 atgaattctg cagtcagttc cggcagttca cgctcttcaa ctttcttcag gttgatagcg
    12481 aatttcgctg ctttaccttt caggttttct gcgtggtact cttccgggaa ggtcacgtcg
    12541 atggtgaact cttcgccagc tttgtggcct ttgataccgt cttcaaagcc cgggatcatg
    12601 cgaccctgac ccatcgccag tacgaaatca gacgctttac cgccttcgaa ctcttcgccg
    12661 tctacagaac cggtgaagtc gatggtcaca cggtcttctg cttcaacagc gccgtctttt
    12721 tctttccagg ttgcctgctg tttacgcaga gtatccagca tgccgtcaac gtcagcgtcg
    12781 gtcacttcaa cgatcggttt ttcaacttcg atcgcttcca gaccctgcag ttcaacttcc
    12841 ggataaactt caaactctac agagtaggtg aagtcttcac ccagcttgta ttcgcccgga
    12901 acataggtcg gtgcgccagc cggattgatt ttttctttaa tgatggcgtc aatgaagtta
    12961 cggctcatca ggtcgcccag gacgtcctgg cgtacagacg cgccataacg ctgagcaacg
    13021 atattcattg gcactttgcc tttgcggaag ccgtcaatac gtactttttt cgcaacgttg
    13081 accagctcgc ttttaacagc ggtctcgatg ctgtcagcag cgatagtaat cgttacacgg
    13141 cggccaaggc cttgagtggt ttcaactgaa acttgcatct tgttacctca aaaaatcaca
    13201 gtgctcggtc aactcttacc ccgcaagcac aggttgttgg cttcgcggaa ccgggatgtt
    13261 cttataatca atcacattcc ctgttaccag aatcatcccg aaagcattca aataggacgc
    13321 ggcattatag cggcatcact tttatgagtc gagaacggtt attgcgcgct gtcgcgtatt
    13381 ttttcacaat tcccgggcga ttttcgtctg aatgctgggc aaaaagcgca aaatccagac
    13441 aaaacaaaac ggcccgcagg ccgtttttca taaaatcttt agcaacatac tggaaaagcg
    13501 ccgacagttg caaatgcgtt tttacgcgat gcttcctgct ccacgacagg gaggagaggc
    13561 aaagacggtg tcctgcaacc cttcccactc cttaatagtg taagtatgca gagccagcgc
    13621 atgaacggta gtagagagtt cctccgctaa agtactgtaa atcattcgat gacgattcag
    13681 aaaacgttca cccgtaaaac gatcgctgac cagcacaact ttaaaatggc tttcagagcc
    13741 ggctgggaca ttgtgacgat agctttcatc cactacttcg aggaatacgg gttggaacgc
    13801 cgcccttaat ttttcttcta tccgctcacg tatcatcatg aaatatctcc tccgacaacg
    13861 ctgagatgtc atccatccct ttaaatacta gccgctttta ccgtttccat tgcagcttaa
    13921 caacaaatat ttaggtttcg tcctggtttt tccgtcaagc gtatgaagtt tactgattta
    13981 aagattgaaa cacaggcctc gcatcagcgt ttcttttacc aggggcgatt atttcgtcaa
    14041 catgatgaat ttacatgcct tacccacttc ccctcgccgt tggcaatgtt atgatggcgg
    14101 gaatttttac caaacacgct taagagtctc tgagagcccg aacatgttca aaaaaatcct
    14161 cttcccgtta gttgctctgt ttatgcttgc aggatgcgca aaaccgccaa caactatcga
    14221 agtttccccg acgattacac tgccacagca ggatccaagc ctgatgggcg tcaccgtaag
    14281 cattaatggt gccgatcagc gtaccgatca ggcgctggca aaagtcaccc gcgataatca
    14341 aatcgttacc ctgaccgcct cccgcgatct gcgtttcctg ctgcaagaag tgctggaaaa
    14401 acagatgacc gcccgtggat acatggttgg tccgaatggc ccggttaatc tgcaaatcat
    14461 cgttagccaa ctgtatgctg acgtgtccca gggcaacgtg cgctacaaca tcgcgaccaa
    14521 agcggatatc gccatcatcg ctaccgcgca gaatggcaac aaaatgacca aaaactatcg
    14581 tgccagctac aacgttgaag gtgcattcca ggcctccaac aaaaatatcg ccgatgcggt
    14641 taacagtgtg ctgaccgata ccatcgctga tatgtctcag gacaccagca tccacgaatt
    14701 catcaagcag aacgcgcgtt aatttctgcc ctctggcccg gtgcaagccg ggcctgtaga
    14761 cacccatgtc cagtcaatat ttacgtattt ttcaacagcc gcgttcagcc atattgctga
    14821 tcctgggttt tgcttccggg ctaccgctcg ccctgacgtc cggcacctta caggcctgga
    14881 tgacggtcga gaatatcgat ctcaaaacca ttggtttctt ctctctggta ggccaggctt
    14941 acgtttttaa attcctctgg tcaccactga tggaccgcta cacgccccca tttttcgggc
    15001 ggcggcgcgg ttggctgctc gccacgcaaa ttctgttatt agtcgccatt gcggcgatgg
    15061 gttttctcga accaggcacc cagctccgct ggatggcggc actggcggtg gtgatcgctt
    15121 tttgctctgc ctcccaggat attgtcttcg atgcgtggaa aaccgatgtg ctcccggcag
    15181 aagaacgtgg tgcgggcgcg gcaatcagcg tgctgggtta ccgtttaggg atgctggttt
    15241 ccggcggctt agccctgtgg ctggcagata aatggctggg ctggcagggc atgtactggt
    15301 tgatggcggc gctgttgatc ccctgtatta tcgcgacgtt gcttgcacca gaacccaccg
    15361 acaccattcc tgtgcccaaa acgctggaac aagcggttgt tgcacctctg cgagatttct
    15421 ttggtcgcaa taatgcctgg cttattttgc ttcttatcgt gctgtataag ctgggcgacg
    15481 cattcgctat gagcctgaca accacgtttt tgattcgcgg cgtcgggttt gatgcgggtg
    15541 aagtaggcgt ggttaacaaa acgcttggct tactggcgac cattgttggc gcattgtacg
    15601 gtgggatttt gatgcagcgc ctgtcgctgt tccgtgcact gctgattttc ggcattttac
    15661 aaggtgcatc taacgccggt tactggctgc tgtcgattac tgataagcat ctctacagca
    15721 tgggtgcagc cgtctttttc gaaaacctct gtggcgggat gggtacatca gcctttgtcg
    15781 cgctgttaat gacgctgtgt aataagtcat tttccgctac tcaatttgcc ctgctctcag
    15841 cgctttctgc tgtagggcga gtttatgtcg gccccgtggc gggttggttt gttgaagcac
    15901 atggctggtc gacattctat ctattctccg tcgccgctgc cgtaccaggg cttattttgc
    15961 tgctggtttg ccgccagacg cttgaatata cacgagtaaa tggcaacttt atctcccgta
    16021 ccgaatatcc tgcaggttat gcctttgcca tgtggacact ggcggcgggt atcagcctgt
    16081 tggccgtgtg gttactgctg ttgacaatgg acgcgctgga tttgacgcac ttctctttcc
    16141 tgcctgcgct gctggaagtc ggggttttag tcgccctttc tggcgttgtg cttggtggtt
    16201 tgctggatta tctggcgcta cgaaaaacac atctgacgta atctgtaaat attatttagc
    16261 tgtagtaatc actcgccgta attattacgg ctaaataaac atcagcatcg cttattaata
    16321 tttgttcttt tgtgcggctt agcgtttgga aattattgac gcaatttatg aattctatt
//
LOCUS       DADSZD010000099        16335 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.100, whole genome shotgun sequence.
ACCESSION   DADSZD010000099 DADSZD010000000
VERSION     DADSZD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.100"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            107..211
                     /locus_tag="J1Z28_002903"
     CDS             107..211
                     /locus_tag="J1Z28_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBA7717720.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            521..2719
                     /gene="speF"
                     /locus_tag="J1Z28_002904"
     CDS             521..2719
                     /gene="speF"
                     /locus_tag="J1Z28_002904"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBA7717721.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRI
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2716..4035
                     /gene="potE"
                     /locus_tag="J1Z28_002905"
     CDS             2716..4035
                     /gene="potE"
                     /locus_tag="J1Z28_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBA7717722.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4092..5732)
                     /gene="pgm"
                     /locus_tag="J1Z28_002906"
     CDS             complement(4092..5732)
                     /gene="pgm"
                     /locus_tag="J1Z28_002906"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBA7717723.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGMPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5758..6303)
                     /gene="seqA"
                     /locus_tag="J1Z28_002907"
     CDS             complement(5758..6303)
                     /gene="seqA"
                     /locus_tag="J1Z28_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBA7717724.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6488..7252
                     /gene="ybfF"
                     /locus_tag="J1Z28_002908"
     CDS             6488..7252
                     /gene="ybfF"
                     /locus_tag="J1Z28_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBA7717725.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7323..7685
                     /gene="ybfE"
                     /locus_tag="J1Z28_002909"
     CDS             7323..7685
                     /gene="ybfE"
                     /locus_tag="J1Z28_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBA7717726.1"
                     /translation="MYYGALSIRAGAWLIVSPEVTKIMAKEQTDRTTLDLFAHERRPG
                     RPKTNPLSRDEQLRINKRNQLKRDKVRGLKRVELKLNAEAVEALNELAESRNMSRSEL
                     IEEMLMQQLAALRSQGIV"
     gene            7825..8355
                     /gene="fldA"
                     /locus_tag="J1Z28_002910"
     CDS             7825..8355
                     /gene="fldA"
                     /locus_tag="J1Z28_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBA7717727.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8565..8651
                     /gene="uof"
                     /locus_tag="J1Z28_002911"
     CDS             8565..8651
                     /gene="uof"
                     /locus_tag="J1Z28_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBA7717728.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8644..9090
                     /gene="fur"
                     /locus_tag="J1Z28_002912"
     CDS             8644..9090
                     /gene="fur"
                     /locus_tag="J1Z28_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBA7717729.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9174..9500)
                     /locus_tag="J1Z28_002913"
     CDS             complement(9174..9500)
                     /locus_tag="J1Z28_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717730.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFAEQESVRVLDYQQCIQATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="J1Z28_002914"
     CDS             complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="J1Z28_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBA7717731.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTVWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAIFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11397..13061)
                     /gene="glnS"
                     /locus_tag="J1Z28_002915"
     CDS             complement(11397..13061)
                     /gene="glnS"
                     /locus_tag="J1Z28_002915"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBA7717732.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13281..14042)
                     /locus_tag="J1Z28_002916"
     CDS             complement(13281..14042)
                     /locus_tag="J1Z28_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7717733.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(14044..15030)
                     /locus_tag="J1Z28_002917"
     CDS             complement(14044..15030)
                     /locus_tag="J1Z28_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HBA7717734.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAAAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(15023..16258)
                     /locus_tag="J1Z28_002918"
     CDS             complement(15023..16258)
                     /locus_tag="J1Z28_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBA7717735.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTVIPCQPVTVSQLSHLSYEQPWISVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPRITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMAIASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
BASE COUNT         3963 a         4165 c         4041 g         4166 t
ORIGIN      
        1 attatttggg atatgatcgt tttttgttga tcttcttcac agattatagc catttcatgg
       61 atagaataac cctaccttca actgacacag aaaaaggtaa aggtaaatgg aaaataataa
      121 ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag
      181 ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac cccagcgtac
      241 aggtctccct gactttaagc attacaggtt aatacctgta tccctcggtg ctcatatact
      301 gctaaccctt ttaaactcta aataattcga gtcgcagcat aggcaacttg aagtatgacg
      361 agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat
      421 ttatttatca gcggtggggt tttgcttttt tccggcgtga tcaatttctc ctttgagaaa
      481 ttgaggacct gctattacct gaaataaaga gatgaacaaa atgtcagaat taaaaattgc
      541 cgttagccgt tcttgcccgg attgtttttc cactcatcgt gaatgcgtga atattgacaa
      601 aagtaattat attgacgttg ccgccattat tttatcagtc aatgatgttg aacgtggaaa
      661 actcgatgaa atagacgcta ctggctatgg cattcctgtt tttattgcaa cggaaaatga
      721 agaacgtgta ccagcagaat atctgccacg tatttctggt gtcttcgagc attgcgaatc
      781 acggaaagag ttttatggac gtcagttaga aaccgctgcc agccattatg aaacgcaact
      841 gcgcccgccc ttcttccgcg cactggtcga ttatgtcaat cagggcaata gcgcttttga
      901 ctgcccgggt catcagggag gtgagttttt ccgtcgccat ccggcaggca atcagttcgt
      961 ggaatatttt ggcgaaatgc tcttccgctc tgacttgtgt aatgccgacg ttgcaatggg
     1021 cgatctactg attcatgaag gtgcgccatg cattgcacag caacatgcgg ctaaagtatt
     1081 taatgccgat aaaacgtact ttgttttaaa cggcacctcc tcctccaaca aagtggtttt
     1141 aaacgccctg ctgacaccgg gtgatctggt gctgtttgac cgcaataacc ataagtctaa
     1201 ccaccacggg gccttgctac aggctggtgc aacaccggtt tatctggaaa cagcacgtaa
     1261 cccgtatggt tttatcggtg gcattgatgc gcactgcttc gaagaaagtt atctgcgtga
     1321 actgattaca gaagttgcac cgcaacgggc aaaagaggcg cgtccattcc gcctcgctgt
     1381 gattcagtta ggcacctacg acggtactat ttataacgcc cgccaggtgg tggataaaat
     1441 cggtcatctg tgtgactaca tcctgtttga ctcagcatgg gtcggctatg aacagtttat
     1501 tccgatgatg gcggactgtt cgccactgct gctggagctt aatgagaacg atccgggtat
     1561 tctggttacg caatctgtgc ataaacaaca ggctggtttt tctcagactt cacaaattca
     1621 taaaaaagac agccatatca aagggcaaca gcgttatgta ccgcacaaac gcatgaacaa
     1681 cgcatttatg atgcacgcct ccaccagccc gttctatccg ctgtttgccg cactggatat
     1741 caacgccaaa atgcatgaag gtgtcagcgg tcgtaatatg tggatggatt gtgtggttaa
     1801 cggtattaat gcccgcaaat tgatcctcga taactgccag cacattcgcc cgttcgttcc
     1861 tgaactggtg gatggtaaac cctggcagtc gtatgaaaca gcgcaaattg cggttgatct
     1921 gcgcttcttt aaatttgtac ccggtgagca ctggcattct tttgaaggct acgcagagaa
     1981 ccaatacttt gtcgatccgt gcaaactgtt gctgacaact ccaggtattg atgcacgtaa
     2041 cggcgaatat gaagcgttcg gtgtacccgc aacgattctt gctaacttcc tgcgcgaaaa
     2101 tggcgtagtg ccggaaaaat gcgatcttaa ctccatcctc ttcctgctga ctccggcaga
     2161 agatatggcc aaactgcagc aacttgttgc cctgctggta cgcttcgaaa aactactgga
     2221 agctgatgcg cctctggcgg aagtactgcc ttccatctac aaacagcatg aagagcgcta
     2281 cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg cccgccacaa
     2341 cgtgaaacaa ctgcaaaaag agatgttccg taaggaacac ttcccacgca tcagcatgaa
     2401 tccgcaagaa gccaactacg cctacttacg cggtgaagtg gagctggttc gtctgccgga
     2461 tgcagaaggc cgtatcgctg ccgaaggtgc gcttccttat cctccgggtg tgctgtgtgt
     2521 tgttcctggt gaaatctggg gtggtgctgt tctgcgttac ttcagcgctc tggaagaagg
     2581 catcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg aagaacatga
     2641 tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa gcaccctgtt
     2701 gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggtgtcgt tcagttaacc
     2761 atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac aaagcttgcc
     2821 gaagtcggga caatctcaat tatctcctgg ctggtgacag ccgtaggctc aatggcactg
     2881 gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggaggtat gggcggttat
     2941 gccgagtatg ctttcggtaa atccggtaac tttatggcga actataccta cggcgtctcg
     3001 ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac cgaactgctc
     3061 ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct gtggatttgt
     3121 accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat taccgtgtgg
     3181 ggggtcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt tagcccgaca
     3241 ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcggt aggttcttcc
     3301 atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa tactgatgta
     3361 gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt aggtgcggcg
     3421 gtgatttata tcgtctccac caacgtgatt gccgggattg tgccaaatat ggagctggca
     3481 aattcaacgg caccatttgg tctggccttc gcgcagatgt tcacgccgga agtgggtaaa
     3541 gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg gcagttcacc
     3601 attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat tttctcccgt
     3661 gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat ccagagcggg
     3721 ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct ggttaacctg
     3781 gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt cattattcag
     3841 aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc ttttgttggc
     3901 gcgatgtaca gcttttatgc gctgtactca tccggggaag aagccatgct atacggttcc
     3961 atcgtgacct tcctcggttg gacactgtat ggtctggtct caccacgctt tgaactgaaa
     4021 aataaacacg gttaattaag caaagcaaaa aagggcgatc ttccgaccgc cctttttctt
     4081 atttgatatg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc ttctttctca
     4141 atctgcttgc gatgttcttc accaaggaag ctttcgcagt agatcttata tgcgtcttcc
     4201 gtgcctgacg gacgcgcggc gaaccagccg ttgtcagtca tcactttcag accgccgata
     4261 gaagcgccat tacccggtgc cgccgtcaga cgcgcggtga tcgggtcacc tgccagggtg
     4321 ctggcgctca ccatttccgg agacagctta gacaacgccg ctttttgtgc ggaagtcgct
     4381 gctgcctgca aacggttata gctcggcgca ccaaagcgtt ttgccagttc gttatagtgt
     4441 tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca cataatgatg
     4501 ccgtctttat cggtggacca cggcatgccg tcgaaacgca ggaaggaagc gcctgcactc
     4561 tcttcgccgc caaagccgaa gctgccatcg aacaaaccat caacaaacca tttgaaacct
     4621 accggtactt ctaccagctt acggcccaaa tcattgacca cacggtcgat catcgcagat
     4681 gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg ctggaacagg
     4741 tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg agtgacgata
     4801 ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc acgcagtgcc
     4861 agcaggcccg ccatcgcaca ctcggaggag cagtccatac ggatcgcacc gtctttatcg
     4921 aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag gttgaggttg
     4981 taatactcgc caatgcgctt ccagtattcg ataccggaac cacccagcgg atcaacgccc
     5041 agcgtcaggc ccgctttctg aatcgccgcc atatcaacga tatcggccag cccttccaca
     5101 aacggctgca ccagatcctg ctctttcaca tgaccggatg ccatcgcttc gtcgagggag
     5161 atacgcttca cgcctttcag gccatcagcc aacagtgcgt tggctctgtc ttccaccact
     5221 ttagtgacgt tggtatcagc cgggccacca tttggcggat tgtacttgat accaccatct
     5281 tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagtgggcc acctttttta
     5341 ttgtgaacca gaatggcatt ggaaatggca ggtgtcggag tgaagccatt gttttcctgc
     5401 acaatgacat caacgccgtt cgctgccagc acttccagta cggaaatgaa cgcaggctcg
     5461 gacagggcgt gagtatcttt acccacatag caagggccag tgatgccgtt tttcgcacgt
     5521 tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct gtggcgcgct
     5581 gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct
     5641 ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc actctgttgt
     5701 gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg caacgtttta
     5761 gatagttccg caaaccttct caatcaattc cgccgggaat tgcatcgact gcatgatgtg
     5821 ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat acggcgtacc
     5881 aggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt catctgccgc
     5941 aaagtaaacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc
     6001 aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct tttgctctgc
     6061 gtattcgtcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac
     6121 cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca ccggagcagc
     6181 aggctgtgat gcggcggaaa atttcaacat acgccgtaaa atgtcggatg cgctctcgcc
     6241 gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt caatcgtttt
     6301 catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaggagtat atgaataaat
     6361 cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa ccttatttgt
     6421 tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa
     6481 ctttgccatg aaattgaata tccgcgcgca aactgcacaa aaccagcaca ataattctcc
     6541 catcgttctt gtccatggtc tgtttggcag cctcgacaac cttggcgtac tggctcgcga
     6601 tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc tttcaccgag
     6661 agatccggta atgaattacc cggcgatggc gcaggatctt gttgataccc tggatgcaca
     6721 gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg taatggctct
     6781 tactgcacta gcccccgatc gcattgacaa gctggtggcg attgatattg cgccggtcga
     6841 ttatcacgtt cgccgtcatg atgagatttt cgcggccatc aacgcagtca gtgaatcaga
     6901 cgcacaaaca cgccagcaag cagcggcaat aatgcgccag catcttaatg aagaaggggt
     6961 gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg tgccagtatt
     7021 gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg atcatcctac
     7081 cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg atgatttact
     7141 ggcacaattt ccacaggctc gggcgcatgt gattgcaggc gcgggtcact gggtccatgc
     7201 tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg
     7261 attaaaaacc cagcgtccgc gtcgctttcc gcgcgtggac gttggcgtga ctcatcgcgc
     7321 tgatgtatta tggcgcgcta tccatccggg caggcgcctg gctgattgtt tcccccgaag
     7381 tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg ttcgcgcacg
     7441 agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgagcag ctgcgtatta
     7501 ataaacgcaa ccaactaaaa cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc
     7561 tgaacgcgga agctgtcgag gcactgaacg agctggcgga atcgcgcaat atgagccgta
     7621 gcgagctgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt cagggcatcg
     7681 tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa
     7741 gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat
     7801 aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg cagcgacacc
     7861 ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat
     7921 gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag catatgacat tctgctgctg
     7981 ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt cttcccgact
     8041 ctcgaagaga ttgatttcaa tggtaaactg gttgcgctgt ttggttgtgg cgaccaggaa
     8101 gattacgcag aatacttctg cgatgcatta ggcaccatcc gcgacatcat tgaaccgcgc
     8161 ggcgcaacta tcgttggtca ctggccaacc gcgggctacc acttcgaagc gtcaaaaggt
     8221 ctggctgatg atgaccactt tgtcggtctg gctatcgacg aagatcgtca gccggaactg
     8281 actgctgaac gtgtagaaaa atgggttaaa caaatttctg aagagctgca tctcgacgaa
     8341 attctcaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt aatagataat
     8401 gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctgt acaatgtccc
     8461 agtgttcaag tggccttgct gttgtaaatg taagctgtgc cacgttttta ttaacaatat
     8521 ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata cgcattatct
     8581 caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca ggacagattc
     8641 cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac gcttcctcgc
     8701 ttgaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc ggaagattta
     8761 tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata tcgcgtactg
     8821 aaccagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta
     8881 tttgaactaa cacagcaaca tcaccacgat cacctgatct gcctcgactg cggtaaggtt
     8941 atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaatcgccgc aaaacatggc
     9001 attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg cgattgccgc
     9061 gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag ccaacctgca
     9121 ggttggcttt tttatacaag ggaaataaga aaagcgagag ttacagctct cacttatttg
     9181 ttattactgc gaatttcctg ccagacctta tcacaatcgg ctttcaccgc ctgatcattt
     9241 ccggtttgcg ttgcctgaat acactgctga taatccagta cgcggacact ttcctgctcg
     9301 gcaaactgct gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca
     9361 attttttccg gcgaaccttg cgcggtgttg atacaggcgc tgtaagcctc tttcagacga
     9421 ctgtcttcct gcggtgcggt tgattgcgca caagctacca gccccgatgc catcatggcg
     9481 atgagaatca gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca aaacctgccg
     9541 cgtcgggcat cagaagatgg tgaatggtgc gattaccatg aatttcacgt cgcgctcatc
     9601 ctggaaaatg ttgccgtaac caccgcccca gcttgggatg tcggagtggt tgtcgtattc
     9661 ggtgaagtgc agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtacaccgc
     9721 atccaggctg taggcagact cttcaatagt ccggttttta tcgtagtacg catccggatt
     9781 gctctgccag gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa
     9841 gcctggaaga ttccagtttt tcaggtcata catcgcaccg aagaagatcg ctttttcgcc
     9901 gttggcgttg aagtcagaac ggttatccca ccagatatcc aggcgaccgt tcgaggaagc
     9961 gtaggttgga gtcatacgtt gcaggaagta tccttgctga ccgtcagcct taacccaggt
    10021 accttcgagg cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcctgcag
    10081 ccagacggtg ccgtcataaa gatcgttgac gctgcgatcg tcaactttat cgcgcgtacc
    10141 atagaactgg tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt
    10201 ggcaaaatat tgatcgatat acccttcggc ctgaccaaac gccgcttcca gtacgaagtt
    10261 atttttgaag tcgtacttcg ccccaaggga gtgtagataa tcaactttgg tggttttatc
    10321 gttctgataa aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat
    10381 gtaggagaaa ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg cctccgcccc
    10441 ctgataagta cccggcataa agctccagtg tggcgctaac agcgtctgac cggttggctg
    10501 aatataacct gccctcgccc aaaccggacc gtatttaaat ttggctgctg ctttatacag
    10561 gcttataccg cttttatcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa
    10621 cgcgatttcg ttcgggtgag agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc
    10681 ggcaatatca aggccgaaca tatcagcagc ataaccagac tgaaaatcga ggttggcgtt
    10741 ccaggtggag tgagaaaggt tggttttgta tttatcgccg tcggtaacat ctttacggtc
    10801 gcgttcacgc tgccagtaat agataccgcc ggttaaggtt gaatcgtcga tgaatccttc
    10861 ggcgcgagcc tccggaattg ccataaagcc cgacatagct gtaacaccgg cgatagccag
    10921 cgccagcgta ctacgtttgc cactaaacgc acgcatgggt taatcctctt tgacgtataa
    10981 attgctgcac caaaggtgaa cagcgaaaaa aataaaaatt aaaaaagtag cgttgagaaa
    11041 agtctcgctg actacaggaa cagactattt ttcgcgacgc gaattatcaa tctctttctg
    11101 taaccagaat gtaagaacat gagcaaccgc acatattttg atgatttttg ttacaaggct
    11161 taagtttgca gttgaaaggc ttgcagaaga ataaaaacgt gaaattccag caagatgaaa
    11221 gcggttacat tttatttaat tatgttttgt ggttttaaat ttattttatg gataaattgc
    11281 gaagcggaat taattcgcga agaaaagcag aaaaaacgcc gcagagcgcg gcgtttggga
    11341 taatttgcat atttcttgcc ggatgcggcg taaacgctct atccgacaac gtaaacttac
    11401 tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac cggtttttcc
    11461 gccgtagaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg gaatgcttta
    11521 cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac cagcgactcc
    11581 gggttaatca ccgacaggaa atcatccgca gcaccagggt tcggcacgct gaacagacga
    11641 tcgtacaggc ggatttcaac cggcagcgca tgtgccgcgc tcacccagtg aataacacct
    11701 ttgactttac gaccatctgc cggatctttg cttaaggtgt cggcgtcata agtacagaag
    11761 atggtggtga tattgccttc ggcatctttc tccacgcgtt ctgccttaat gacgtaagca
    11821 ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt agcttcttcg
    11881 cggaaatcgg cgcgatcgat ccaaatctca ccgctaaatg gcacctgacg gctgcccatt
    11941 tccggtttat tcggatggtt cggcatggta accatttcgc cttcgccctg gtagttttcg
    12001 ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gcgcattttc gttgagatct
    12061 tcacggatgc aggattccag tgacgccatc tcaatggtgt tgtcctgctt ggtcacacca
    12121 atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg cagaccagaa
    12181 atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac cagcaggttc
    12241 agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc gtactggcgc
    12301 gggtgaacag gaatggtgat gttgtccagt acccagtcgt acagacggcg gttgtcctgg
    12361 aactcaagcg tacacagaga gtgcgtaata ccttccagcg catcgctgat gcagtgggtg
    12421 aaatcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc agcaaactta
    12481 atgcggtaca gcaccggatc gcgcatcacg ataaacggcg aagccatgtc gatcttcgca
    12541 cgcaggcagg ctttaccttc ttcaaaaccg ccggtacgca ttttctcgaa cagcgccagg
    12601 ttctcttcaa cgctgcgatc gcggtacggg ctgtttttac ccggctgcgt caaggtgccg
    12661 cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc tttattgatc
    12721 agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg gacgttacca
    12781 gaccagtgga aacctaacca ctctacgtcg tttttgatcg aatcgacata ctcgatatct
    12841 tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata gtcctgggcg
    12901 atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc attcggctcc
    12961 ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc atcgatgatc
    13021 tgacgaataa agttagtcgg gcgggcttct gcctcactca tcgtagattc ctcaaagcgt
    13081 aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact gttagttagc
    13141 gaaaaaatgg ttttgcgtaa gatattgggg cgttttctgc gctactgcca gcagatcctc
    13201 gcgctggaaa cgtaaggcca aacgtcgcca gcctgacgca tttattaaat tgccggatga
    13261 gctggccgca gagtcataga ttagtcggct atgattaaac gcgtcgacaa ggacgcaagc
    13321 gttctacgcg tctgcccatc aagctgacta tccgtaataa tctctgtcag ttcattaaga
    13381 tgcgcgatat taaacagcga ccatgaaccg tattttgagc tgtcagccag gagaattttg
    13441 cgcgaagcat gcgcgataag atcgcgtttt agtgcggctt tctcttccgt gggtgctgta
    13501 ataccctttt caagatccca gctgttacag cttaaaaatg ccacgtccgg ccaggtattt
    13561 tgcagcaatt tacgaccatg ttcgccaatg caggactggc tggagtcgtc gatacgtccg
    13621 ccgataatcg tcacttcaat ttgcttgaat tcagccaaaa aaagcgcgat atgcaggtcg
    13681 cttgtgatca cgcgaagcgg taaatgtgta atctgccgcg caagttcaat cattgtcgta
    13741 ccggcatcaa ggacgacgga atcccccgct ttgaccattg acgccgctgc gctggcgatg
    13801 gcatgttttt ccgccaggct gcgctgcatt ttctcatggg tagtcggttg ggtaggaata
    13861 aaccggttga gggtcacacc gccatgtgta cggctgataa caccctgctc atcgagtttt
    13921 atcaggtctc gacgaatcgt agccggcgac gcattggtta ccgccaccag ctcatcaacg
    13981 gtgaccagat tatggctttt caggtaatcc atgatctgct ctaaccggtt atatcccttc
    14041 attttattct cgcgaaatct gcattgctaa ctggatggaa actgccatgc tctcagactt
    14101 ggcttttcct gtccaggcaa tatcaaacgc ggtgccgtgg tcggcagaag tgcgaataaa
    14161 tggcaacccg gcggtgatat tcaccccatc ataaaatcca agcagtttta aagggatatg
    14221 cccctgatcg tggtacatag ccactaccat atcgaatagc ccttcatggc attgcataaa
    14281 cacggtatct ggcgggcacg ggccagtaac atcaatatct tcggcctgca ttgcttcaat
    14341 cgcaggcgcg ataatctcga tttcctcagt gccgaataaa ccatgttccc ctgcatgtgg
    14401 atttacaccc gccacggcaa tacgcggacg ttcaatacca acacgtttca ggaagtggtt
    14461 cgcaacacga ataaccgttt tgaccctttc accattcaag gtatccagga atttacgcag
    14521 tgagatgtga gtcgaaatat ggataacctt gagcttatcg gtatagagca ccatcgcgta
    14581 ctctttgctg ccggtaagat gggccagcaa ttccgtatgc cccggatagt tatgcccgcc
    14641 aagatgcagc gcttctttat tcagcggcgc agtggctatg gctttcacct cgcctgataa
    14701 ggccagagcc gttgcacgtt tgatacagcg atacgctaaa tcgcctgccg cagcctgtac
    14761 cactccgggt gtcagcgcat cggggttagc cagcggctca tccatcacgt taatcacgcc
    14821 gggagcaaaa tgggcgtcgc acaccttttg aatgacgcgt aactctgcct gcggcgtgat
    14881 attcatcgcc ataatacgtc gcagcgtgcg cacacagccc accaccacca ccggggcacc
    14941 agcaagcccg gcttcgctca gggatttaat aatgatctcc gggccaatac ccgccgggtc
    15001 acccatcgtt accgcaataa tattattcac tacacttctc ctcgataaaa ttcagaacct
    15061 gatgcaacgt ggtctcatcg ccaaacccac cggctttcgt catcaccgaa cgggaccaga
    15121 caccgcccag aaagtgaccg tatggaacgc attgcgccac cttccccgta atacgaaatc
    15181 cggtcgcgcc taacgcactg gctattgcca ttgcgacatc accaccagag agataaagcg
    15241 catcgggcat tgtgcgaagc agaacttgcc gggtaagttc gccaagaaac cggcaaattt
    15301 tttccccaag tgcagcgcga ctcatctgcc aacgctggca aagggtgtcg atctgatgcc
    15361 ttgctacaga atcgttacag gtgtggacaa tgcagtggtc gcctttctgc aaggcctgca
    15421 caatacgcgc gtcatagtca ctggcattgc ctgccagaat gtgttcaaca tcgacgtata
    15481 tctgtgtgat acgtgggtgt gaatgaagtg tggcgatctg ccgttgagcg atttcgctca
    15541 ttgaaccaat aatggccagc acggtgggtg gcggcataat cgcggagcgc cgggcaacgg
    15601 catcacaaat accagcggag ccgaccagca gcagcggttg tttagcctgc atcacagcgg
    15661 cagcgatgcg atccaggtcg ctgtccgtct gcgcatctat cacgctaatc cacggttgct
    15721 cataagacaa atggctgagc tgagaaaccg taacgggctg gcatggaatg accgtctgca
    15781 accggatgat gtccgcaatt cgggcactca ataccggggt tttcggatcg ctggcaaact
    15841 cggtttccgt tatccgcaca ccgtttaccc aacactcacc ggccactgtg gtccttcccg
    15901 cctgaggaaa tgcgggggca accactgcgc ccgttatacc gcatgccttc atcagggctt
    15961 caatttctgc gccgatattg cctcgcagcg tggaatctat ctttttaatc acgagcggcg
    16021 ggttattcgc cagagggaga tgactcatca gcgaggttat tttcatcgcc gccagcttag
    16081 gatccatcgc ccggctgtcg ctgttaatca cccatacgct ggcatcaccg cgataaggca
    16141 tttcaaaggc cacatcaact gtatgcccca cctgcgccag gcttaccccc gcgtcgttgg
    16201 ctcccgtgaa atcatcggca acgacgagaa tgctcggctt agtgattccc ttttccatca
    16261 tcactccagg ttgttttatg ataacaatga ttatattcaa tcatgattga ttaaatgtga
    16321 gcaagctcaa gttat
//
LOCUS       DADSZD010000100        16273 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.101, whole genome shotgun sequence.
ACCESSION   DADSZD010000100 DADSZD010000000
VERSION     DADSZD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.101"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            108..1598
                     /locus_tag="J1Z28_002919"
     CDS             108..1598
                     /locus_tag="J1Z28_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBA7717736.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     RADSSFLYDKYQNQSIAAHVMRALAAEQSEVPPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1641..2144
                     /gene="yeaK"
                     /locus_tag="J1Z28_002920"
     CDS             1641..2144
                     /gene="yeaK"
                     /locus_tag="J1Z28_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBA7717737.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(2145..2249)
                     /locus_tag="J1Z28_002921"
     CDS             complement(2145..2249)
                     /locus_tag="J1Z28_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717738.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            2419..2865
                     /locus_tag="J1Z28_002922"
     CDS             2419..2865
                     /locus_tag="J1Z28_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBA7717739.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2822..3643)
                     /locus_tag="J1Z28_002923"
     CDS             complement(2822..3643)
                     /locus_tag="J1Z28_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7717740.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCLLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            3740..4921
                     /gene="nimT"
                     /locus_tag="J1Z28_002924"
     CDS             3740..4921
                     /gene="nimT"
                     /locus_tag="J1Z28_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBA7717741.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4976..5323
                     /locus_tag="J1Z28_002925"
     CDS             4976..5323
                     /locus_tag="J1Z28_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBA7717742.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5345..5599)
                     /gene="yoaF"
                     /locus_tag="J1Z28_002926"
     CDS             complement(5345..5599)
                     /gene="yoaF"
                     /locus_tag="J1Z28_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBA7717743.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5782..6807
                     /gene="dgcP"
                     /locus_tag="J1Z28_002927"
     CDS             5782..6807
                     /gene="dgcP"
                     /locus_tag="J1Z28_002927"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBA7717744.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            6840..6938
                     /gene="yoaK"
                     /locus_tag="J1Z28_002928"
     CDS             6840..6938
                     /gene="yoaK"
                     /locus_tag="J1Z28_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBA7717745.1"
                     /translation="MRIGIIFPVVIFITAVVFLACFFIGGYAAPGA"
     gene            6941..7015
                     /gene="yoaJ"
                     /locus_tag="J1Z28_002929"
     CDS             6941..7015
                     /gene="yoaJ"
                     /locus_tag="J1Z28_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBA7717746.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(7075..7323)
                     /locus_tag="J1Z28_002930"
     CDS             complement(7075..7323)
                     /locus_tag="J1Z28_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBA7717747.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7471..7653)
                     /locus_tag="J1Z28_002931"
     CDS             complement(7471..7653)
                     /locus_tag="J1Z28_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBA7717748.1"
                     /translation="MGKATYTVTVTNNSNGISVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7657..8016)
                     /gene="yeaR"
                     /locus_tag="J1Z28_002932"
     CDS             complement(7657..8016)
                     /gene="yeaR"
                     /locus_tag="J1Z28_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBA7717749.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8189..8827)
                     /gene="leuE"
                     /locus_tag="J1Z28_002933"
     CDS             complement(8189..8827)
                     /gene="leuE"
                     /locus_tag="J1Z28_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBA7717750.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8954..9121)
                     /locus_tag="J1Z28_002934"
                     /pseudo
     CDS             complement(8954..9121)
                     /locus_tag="J1Z28_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            9110..9280
                     /locus_tag="J1Z28_002935"
     CDS             9110..9280
                     /locus_tag="J1Z28_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HBA7717751.1"
                     /translation="MTTGGKQPGDIDFYVVRKNTEGEYSSPGGRVNEGTENEVVIQES
                     VFTRRGVERILR"
     gene            complement(9370..10485)
                     /gene="rnd"
                     /locus_tag="J1Z28_002936"
     CDS             complement(9370..10485)
                     /gene="rnd"
                     /locus_tag="J1Z28_002936"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBA7717752.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNVWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10567..12252)
                     /gene="fadD"
                     /locus_tag="J1Z28_002937"
     CDS             complement(10567..12252)
                     /gene="fadD"
                     /locus_tag="J1Z28_002937"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBA7717753.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(12457..13038)
                     /gene="yeaY"
                     /locus_tag="J1Z28_002938"
     CDS             complement(12457..13038)
                     /gene="yeaY"
                     /locus_tag="J1Z28_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBA7717754.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(13078..13773)
                     /gene="tsaB"
                     /locus_tag="J1Z28_002939"
     CDS             complement(13078..13773)
                     /gene="tsaB"
                     /locus_tag="J1Z28_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBA7717755.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLLDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13831..15741)
                     /locus_tag="J1Z28_002940"
     CDS             complement(13831..15741)
                     /locus_tag="J1Z28_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBA7717756.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIELAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15873..16217
                     /locus_tag="J1Z28_002941"
     CDS             15873..16217
                     /locus_tag="J1Z28_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBA7717757.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFDQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3997 a         4187 c         3966 g         4123 t
ORIGIN      
        1 ccctccccag cggcgcagag atgagtataa ttagcgcccc tgtgccaggc cgcaatcgaa
       61 ctttatctgg ttttctcgtt tcactaaccg aaggagtgcc atttatcatg aaattgcacc
      121 atagaatgct ccggcatttt atcgccgcaa gtgtcattgt gctgacatct tccttcctta
      181 tttttgaact tgtcgccagc gacagagcaa tgagtgccta tctgcgctat atcgtgcaga
      241 gagcagactc ttcctttctt tatgataagt atcagaatca gagtattgcc gcgcatgtga
      301 tgcgcgctct cgctgctgag cagtcggaag tgccgccaga acagcggcgc gccatctgcg
      361 aggcttttga gtctgccaat aatacccatg gcttaaacct gactgcccat aaatacccgg
      421 gcttacgcgg cacactacaa accgcatcca ctgactgcga cacaattgtg gaagctgcag
      481 cactattacc cgcttttgat caggcagtgg aaggcaaccg ccaccaggat gattacggtt
      541 caggtcttgg gatggccgaa gagaaatttc actattatct cgatctcaat gaccgctatg
      601 tctattttta tgagccggtt aatgttgaat actttgcgat gaataactgg tccttcctgc
      661 agtcaggaag tattggtatc gatcgcaaag atattgaaaa agtatttacc gggcgtaccg
      721 tattatcgag catttaccag gatcagcgta ctaaacagaa cgtgatgagt ttgctgacgc
      781 cggtatatgt cgcagggcag ctaaaaggga ttgtgctgct ggatattaac aaaaacaatc
      841 tgcggaatat cttttacact catgaccgcc ctctcctctg gcgttttctc aatgtcacgc
      901 taaccgatac agattcgggg cgcgacatta tcatcaacca gagcgaagat aatctgttcc
      961 agtatgtcag ttacgtccat gacttaccgg gcggcattcg tgtctcgtta tccattgata
     1021 ttctttactt tatcacgtct tcgtggaaaa gcgttctgtt ctggattttg acggcgttaa
     1081 ttttgctgaa tatggtgcgg atgcacttcc gcttatacca aaatgtgtcg cgagaaaata
     1141 ttagtgatgc gatgactgga ctctataatc gcaaaatttt aacccctgaa ctggagcagc
     1201 ggttgcagaa actggtgcaa tccggttctt cggtgatgtt tattgctatt gacatggaca
     1261 agttaaagca aataaatgac accctcggtc atcaggaggg ggatttagcg attacgttat
     1321 tagctcaggc gattaaacag tcgattcgta aaagtgatta tgccatccga ctcggtggcg
     1381 atgaattctg catcattctt gtcgattcga cgccgcaaat tgcagcacaa ctgcctgaac
     1441 gtatcgaaaa acgtctgcaa catatcgcgc cgcagaaaga gatcggcttc tcttccggta
     1501 tttacgcgat gaaagaaaac gatacgttac atgatgcgta taaagcttcc gatgagcgtt
     1561 tatatgtcaa taagcagaac aaaaacagcc gttcatgata atcttctgtg gctgtttgct
     1621 tgtaatctca ggagcatgaa atgactgaaa tggctaaagg aagcgtgacg catcagcggt
     1681 taatcgcttt attatcacaa gaaggtgcta acttccgcgt cgttacccat gaagcggtag
     1741 gtaaatgtga agcggtgtca gaaattcgtg gcaccgcttt aggccagggg gctaaggcac
     1801 tggtatgcaa agtcaaaggc aatggcgtaa atcaacatgt tctggcgatc ctcgctgccg
     1861 atcaacaggc cgatctgagc caactcgcca gccatatagg cggattacgc gcttcgttgg
     1921 ccagcccggc ggaagtcgat gaactcacag gctgtgtctt cggcgcgatc ccccctttca
     1981 gcttccatcc aaaactcaaa ctggttgccg accccctact cttcgaacga tttgatgaaa
     2041 tcgccttcaa tgcaggcatg ctggataaat ccgttatttt gaaaaccgcg gattatttgc
     2101 gtattgcgca accagaactg gtcaatttcc gccgcactgc gtaactagcc tgtccgttca
     2161 ataagcagaa cggacaaaac cagcacagca gcaatggcaa aaaacgatga cgtgataatc
     2221 agtgtctcga cgaacatttg atcgttcata gtatgccccc gggagatagc tgttttcacg
     2281 ttattgttaa cgtacacaaa tggcagtttg atgacagttc gtctgatttt ttatgcaaaa
     2341 aacgtaaaga ttttctactt ccttcctgca gcaagcgtaa agtaagcagg cttattattt
     2401 tttggcaagg aaaccacgat gtttgatgtc actttgctga tcctgctcgg gttagctgcg
     2461 ctgggcttta tcagtcataa caccactgtc gccgtttcaa ttctggtgtt gatcattgtc
     2521 cgcgtcacac cgttaagcac ctttttcccg tggattgaaa aacaggggct aagtatcggg
     2581 ataatcattc tgacgattgg cgttatggct cccatcgcca gcgggacgct accgccctca
     2641 accctgattc actcgtttct gaactggaaa tcgctggtgg caattgcagt cggcgttatt
     2701 gtctcctggc tgggggggcg cggcgtgacg ttaatgggca gccagccgca actggtcgcc
     2761 gggctgctgg tgggcactgt tttaggcgtg gcgctgtttc gcggcgtacc ggttggcccg
     2821 cttattgccg cgggtctggt ttcgctgatt gtggggaaac agtagttaat cccgcaatat
     2881 atcggcctgg cgtttgcccc aggcctttct taaacatagt aataaaggcc gttgttgaat
     2941 cataccccag cgtatgagca actttctgta cagtatcgcc tttcactaat ccctgaagcg
     3001 ccataatcag ctgtaactgc tgacgccact ggcgaaaact caaccccgtc tctttcacta
     3061 tcagccgggc aagattgcgt tcactcatcg caaaaaagcc agcccattgc cccaatgagc
     3121 cccactcgac gggctctttc gccatcatct ccaccatcgt gcggatttta ggatgagaag
     3181 aaacgggcag gtgcagctgt tgttgtggct gctgagggag ttcatcaaac aggacctgaa
     3241 ttaatctgcg ggtcatcggc tcagcacgct gagtcgtcgt cctgtttgcc agggttaaaa
     3301 ttaattcccg gcataagggc gatattttca atgtgcaaca tgttgtcggc attgtcactg
     3361 cgctgggctc gataaacaga agacagagtt cagcgttagc tgtcacttga ttactatgtt
     3421 tcaccccgcc cggtatccaa accgcatatt gtggcggcac catccacaaa gcattttcca
     3481 ccgtacaggt aattgcacca tgtagtgcca gtatcaactg ccctttgcgg tgctgatgta
     3541 caggactggt taactcatcc gttccggcgt gaatacaaaa tgcgaccgcg gcttcatggt
     3601 gtcggtcggg ttcatagcca ttgagattca acctgtgcat cattttgtcc gaatttagcg
     3661 atattttgtc attttagctt gattcaacat aacaataaaa acggtaaggt acagcctcgt
     3721 ttgtaacaat gagaagcata tgacctgttc aacttcatta agcggcaaaa acaggattgt
     3781 cctgatcgct ggcattctga tgattgccac aacattacgc gtcaccttta ccggcgcagc
     3841 accgttactg gatacgattc gttccgctta ctcgctaacg acggcgcaaa ccggcttatt
     3901 gaccaccctg ccattattag cctttgcgct aatctcaccg ctggccgccc cggtggcgcg
     3961 acgttttggt atggaacgta gcctgtttgc cgcgttactt ttgatctgtg caggtatcgc
     4021 aattcgctct ctcccttcgc cttacttatt atttggcggt acagcggtca ttggcggtgg
     4081 gattgcatta ggcaatgtct tactgccagg attaattaaa cgcgatttcc ctcattccgt
     4141 cgccagactt accggcgcat attccctgac aatgggagct gcggcggcac tgggatcggc
     4201 gatggtagtg ccgctggctt tgaacggttt tggctggcaa ggcgcgttgc tcatgctgat
     4261 gtgttttcct ctgctggctc tttttttatg gctgccacag tggcgaagtc aacaacatgc
     4321 aaatttgagt acctcacgcg ccttacatac tcggggtatc tggcgttcgc cgcttgcctg
     4381 gcaggtaaca ttgtttcttg ggatcaactc actggtctat tacgtgatta ttggctggct
     4441 tccggcgatt ctcatcagtc acggctatag cgaagcacag gcgggttcac tgcatggttt
     4501 gctgcaacta gccacagcag ctcccggttt gctgatccca cttttcttac atcatgtgaa
     4561 agatcagcgt ggtattgcag cgttcgttgc cttgatgtgc gcagtgggcg cggttgggct
     4621 ctgctttatg ccagcacacg cgatcacctg gactctgctt ttcggttttg gttccggcgc
     4681 aacaatgata ctggggttga cgttcattgg tctgcgggca agttctgcgc atcaggcggc
     4741 ggcactctcg gggatggcac aatccgtcgg gtatttgctg gcagcctgtg ggccgccgct
     4801 gatgggtaaa atacacgatg ctaacggtga ctggtccgta ccactgatgg gtgttgcaat
     4861 actttcacta ctgatggcga ttttcggact ttgcgccggg agagacaaag aaattcgcta
     4921 atatccggtg ctatagtgac gtgacaaatc atgcgtgaaa gggagaacaa atacgatgaa
     4981 tattcagtgc aaacgcgttt atgatccggc tgaacagagc gatggttatc gcgtactggt
     5041 cgaccgcctc tggccacgcg gtatcaaaaa aaccgattta gcccttgatg aatgggataa
     5101 agaaatcacg ccgtcaacgg agctgcgcaa agcctttcac ggcgaagtcg tcgattttgc
     5161 aacctttcgc gagcaatatc ttgcagaact ggcgcaacac gagcaagaag gaaagcggct
     5221 ggcagacatc gccaaaaaac agccgctgac cctgctctac tcagcaaaaa acaccacgca
     5281 gaaccatgcg ctggtgctgg cagactggct acgtagcttg tgattttagt acagcatccg
     5341 gcggttattt ttcaccagcc ggatggtccc gtcgccacaa tgcccattca tcaatcgttt
     5401 caccgcccgg taatttgcaa ttgttgctga ccccttgcgc tgtctgcaca ggaatgagcg
     5461 tcccgccctt ctgctggcaa tagaccgacg ccggatttgc cataccaatc tgcggcggtt
     5521 taggtgcttc tggctgagaa ggggttgaac aaccagccag gaccagcaag caaggcagaa
     5581 caaaactgat aattttcatt tattgatctc acatatttat ccaagcttag agtatcgcgg
     5641 tatcgttttg ttttgcagca ctatttttat tacattcact caaaacatat tacgccttgt
     5701 ttcatctttg ttgatgatgt tttatcatgc ctgcaaagat taaataatca gcatttaccc
     5761 gccgtatcct ggagttgttt cgtgtcagat cagattatcg cccgcgtctc gcaatccctt
     5821 gccaaagaac agtcactgga aagtctggtt cgacagcttc tggagatgct ggaaatggtc
     5881 actgatatgg agtcaaccta cctgaccaaa gtggatgtcg aagcgcgcct gcagcatata
     5941 atgtttgccc gtaatagcca gaaaatgcac atcccggaga attttaccgt ctcgtgggat
     6001 tactcgttat gcaaacgcgc cattgatgaa aactgttttt tcagcgatga agtccccgac
     6061 cgttggggcg actgtgttgc ggcacgcaat cttggcatca ccacatttct gagcacgcca
     6121 attcacttac cggatggatc attctatggc acgctttgcg ccgccagcag tgagaagcgc
     6181 cagtggagtg aacgcgcgga acaggtctta cagttattcg ccggactgat tgcacaatat
     6241 attcaaaaag aggcactggt tgaacagctg cgcgaagcta atgctgcgct gattgcgcaa
     6301 tcgtataccg actcgttaac cgggctaccg aatcggcggg caatttttga aaatctgacg
     6361 acgctgtttt ctctcgcccg gcatcttaac cataagataa tgatcgcgtt tatcgatctg
     6421 gataacttca aattaatcaa tgatcgtttt ggtcataata gtggcgatct gtttctcatt
     6481 caggttggcg agcgccttaa tacgctccag caaaatggtg aagttattgg tcgtctcggc
     6541 ggtgatgagt ttttggttgt ttcactgaac aacgagaatg cggatatttc gtcgctgcga
     6601 gaacgtattc aacaacaaat acgcggagaa tatcacttag gtgatgttga tttgtattat
     6661 cctggtgcca gtcttggcat agtagaagtc gatcctgaaa cgaccgatgc agacagtgcc
     6721 ctgcatgctg ccgatatcgc gatgtatcag gagaaaaaac ataaacagaa aacacctttt
     6781 gtcacgcatt cagcgctaca ttcctgaggc gtattcacat ccttttgatt ggtgataaca
     6841 tgcgaatcgg tattattttt ccggttgtaa tctttattac agcggtcgta tttttagcat
     6901 gtttttttat tggcggctat gctgccccgg gagcataaag atgaaaaaaa caacgattat
     6961 tatgatgggt gtggcgatta ttgtcgtact cggcactgag ctgggatggt ggtaacgtca
     7021 catctaaaaa aatagcaaag gctgcctgtg tgcagccttt gtgcaattta cgcgttaact
     7081 tttaatcttc ctgtagataa atagcacaac aatcgcacca ataacggcaa ccacgaagct
     7141 gccaaaattg aagccatcga ctttaccaaa gccaaacagc gtgctgatcc atccgccgac
     7201 tacggcaccg actatcccca gcaggatagt cataaagaat ccaccaccat ctttacctgg
     7261 catgatccac ttcgccagaa taccggcaat aagcccaaaa ataatccatg acagaatgcc
     7321 cattgtttcc tcacttatct gttttgcatt agcgggttag tcgctgataa aaagcatagc
     7381 acaacatcgg gagggtaaga tttgtgacga gcatcacgga ggaatttttt gcgatggcgc
     7441 agaaattgcg ccatcaacga tcagtgataa ttaccaacca caaacatcat gttcgttttc
     7501 cgtgtcataa gaacgcacgg tattcaccag atcttttatc acttcagccg ccacttctgg
     7561 caccagcaaa gtcatcggcg tctctgtttc ataatcgaca gaaatgccat tgctgttatt
     7621 ggtgacggtc acggtatacg ttgctttgcc catgattcat ttcccgttat gaatgacttt
     7681 ccgttgttgc gcaccttcca tcaggacttc aggagcaacg aagaagtcaa tgttgaaata
     7741 agtatcgtca gtcatggctt caatgttgtg ccacttttct ggagggaaca ccgcaaactg
     7801 ccccgcttcg ataaggatca cctgatcagg ctctgcactg tgttcatcag cgtagccgag
     7861 atatttgacc gccccatgca taacggaaag gcgtgggtaa acccctgggc gcgttccttt
     7921 atcaagatga cgttcgaata ttccggcagg cgcagtttgt ttattccaga aaggcgttga
     7981 gcgcgtatga atataattct gtgggatttg cagcatcctt ttccctcctt cggtgaatgc
     8041 gctgaaaacg gtttattcca gccgtttcag ggtacgcctg ataatttgca ttttaaatac
     8101 catttattgg ttacttttta gcaccgtatc agagaagaat cagggagaat tacaggtggg
     8161 aagcccgcgg caacgcgggc tgaaagcatc aggattgcag cgtcgccagt cgggcggcga
     8221 aacccacgaa catcaaacca atcagtgagt tgcccacttt agccagtttc tttttggtac
     8281 gtatgtactg cgtgacaaaa gccccagaaa taatcagaaa gctcaaatag cagaaactca
     8341 ccagttccag cgtcgtcgcc agaataaaga atgaaattcc cgtatgtggg gcattaacat
     8401 cgataaactg tacgaaaaac gacacataga acaaaatggc tttcggatta gtcaggctca
     8461 aaattaacgc gcgtttaaaa atggcaccgt attggggctc atcggatttg gtctcgctat
     8521 ttttaccttt cagggtcgcg tagagaattt tactccccag atagagcaaa taaaacgcac
     8581 caagataacg tacaatgttg aataatatcg gggtggtctt aattaatgtc gccactccag
     8641 cccatgccag aaacatcaat accgcatcgc caataaatac accacaggcc gcaagataac
     8701 cgcctttcat accgctactg acgctatttt tgagtacaaa cagggtattt ggccctggca
     8761 ccaacacaat aaaaatggcc ccaaccagat aggtccagta attcagaacc ccgtattcag
     8821 cgaacacatt aacctcttta attatctttc gatcatgcgc gattaaaggt gaatatgcta
     8881 accaatctgt agcggcttag aaaggagaaa atcaggtttt aacccgatat caacccgata
     8941 attgaatcat taatcaggcc tgctccagtg aaaaattcgg gtagtgctcg gcaaaatact
     9001 ggcgtaaaaa ttctaccgtt atccgcactt tcgctgacgt cgacagcttt gaaacataaa
     9061 cggcccagac attcgctggc tggtaatatt ccggtagcac ttgtactaaa tgaccactgg
     9121 cgggaaacag cctggcgaca tcgattttta cgtggtccgg aaaaacaccg aaggcgaata
     9181 ttcctcgcca ggcggtagag taaatgaagg aacagagaat gaagtcgtca ttcaggaatc
     9241 ggtatttact cgtcgtggtg tcgagcgcat tttgcgttaa gctcaaataa gttcatttga
     9301 accgcctgct gttggatttg tagtttgccg aagccggact gagcgcgccc agtccggctt
     9361 cggaagattt tactgtggat attcctgcaa taaattgtgt aatgcttccg ccatcaactc
     9421 accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt tccagtgcca
     9481 gttcagcagt tggttgattt gccgacgcga cgccagcaat tcggcgctga tcttgtgcgt
     9541 ttcgctgacg tcagtaatca gcgacttaat cgctttaaac gctttacgat aacccggcat
     9601 gtccatcagg ttaagcatcg gctgcggtaa ggcctcttct ggcaatgcct gcgctttttc
     9661 caccagcgcc agcaacgttt taccgtggaa gcggatttcg ctaccggata agcccaggct
     9721 gtccagttcg cccagacttc ccggcatata acgcgctacc gaccacaaat gctcttcacg
     9781 cacgacaaag ttcaccgcca gatcgcgctc tcgcgccttg cgcaatcgcc agtcggctaa
     9841 cagttgcaga caggccagtt ggcgtgtgcg taactgccag acattggtga tatcacgcca
     9901 ggcgtcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcggc attcatccag
     9961 cgccgcaggt agccagccgg aggcctccgt ttctaccata agctttgcgg tgatcggtaa
    10021 cagataccag acatccgccg ctgcatattc acactgacgt tcggtcagcg gtctggccag
    10081 ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt ccaccatgga
    10141 agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg tgtcaatcaa
    10201 gggttgtggt aattcgccaa agacgttgag gaacacttcc agatcttcac tgcctgcatg
    10261 gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc agtcggtgat
    10321 cccgagagga tcaattagcg ccagatgctc accatcaaaa agttgaatta aacccagctg
    10381 cgggtaataa gtgcgcgtac gaacaaattc agtatccagg gctatcgccg gaaaggcacg
    10441 gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt aattcaaatt
    10501 gtattctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg acgactgact
    10561 taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa
    10621 aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt
    10681 gtagcccgtg agctgacggc ggcaaaaggt caccagtgac tcttcggtaa gcgatggatc
    10741 ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc
    10801 cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggataaac
    10861 gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca ggaatccttc
    10921 ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga tttcatcggt
    10981 agcatccgga cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc
    11041 tggttgaccc ggtggtactt cattatcatc atcatccacc agtttggctt ccgtcgacgg
    11101 caccggtaaa ccgatgctac cactatgata atcaatatca tacgggttaa cgctgaccag
    11161 cggcgcgcac tcggtcaggc cataaccttc cagcagatac tgtccagtca gtttcaccca
    11221 acgttctgca accacttgct gaacaggcat cccaccgcct gcggaaagat gcagactgga
    11281 gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg tgttaacgcc
    11341 agtgatagcg gtaaacggat atttcgctaa ctcttttacc agccctggaa tatcgcgcgg
    11401 gttagtgata agcaggttct gcccccccag ttcgataaac agcaggcagt taatggtcag
    11461 tgcaaaaatg tgatacagcg gcagcgccgt caccaccagc tctttgcccg gatgcaacag
    11521 cggaccatag gtcgcgttaa cctgttccag gttcgccagc atattgcggt gagtcagcat
    11581 cgcgcctttc gccacaccag tggtgccgcc ggtgtattgc aggaaagcta aatcttccgg
    11641 caccagttct ggtttgacgt actgcatccg gtagccgttg tgcagggcgc tacgaaatga
    11701 aatggcatct ggcagatggt atttcggcac cagacgcttg atgtatttaa caacgaaatt
    11761 gactaccgtg ccttttgccg tagatagctg gtcgcccata cgggtcagaa taacgtgctg
    11821 aacggcggtt ttatcaacca ctttttccag agtgtgggca aagttagaca cgataacaat
    11881 cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat acaacgggtt
    11941 aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg gatattgcaa
    12001 taagttaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc cttgttgcaa
    12061 ataagcggca aacgcgcgac tgcgttcttc cagtttgcgg aaggtcatca cctcccccat
    12121 attcacaaac gcgggttgat cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag
    12181 agattgataa cggtcagggt tgatctccgt cggaacgtcc gcgggataac ggttaagcca
    12241 aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaagtga
    12301 taaacatgct gttaacataa tattaactca tcttaccagc ttgataatta cccaaggaaa
    12361 aggttgcgaa gcgcgtcact atttattttt atctttaccg taagaatgca gaaacagcgg
    12421 accggccgct gtttcttttt ctttaaaaac aagcaattat tcagttacaa ctgtttgtac
    12481 tctcgcgggg ccgggattat accagcccca tccgccgtag ccatagggcc agccacgacc
    12541 gccataaaac catggatcaa tcggctgagg cggcataatc acctgctggg ttaaatgcca
    12601 acgtttgtaa cccgttactt gcatcaccat aaatttatag ggcgtattac cgattttgcc
    12661 gtcaaccgca ccggtgattg gcccgactac cgtcaccagt tgtccgcgga aatccaccgg
    12721 gtccagaaaa ccgttcacat cggcataaat gcgaccgcga gaaggttctc ccagcgtcgg
    12781 tctggctccg ctgtccagtg gtacggtggc aatttccagg cgggttttcc cttgctggtt
    12841 ttgtaccgca accactttgc cgccaaagcg tgcctcctga ccaacgtaca gctgcggcgc
    12901 actcatcacc cgaactaaat cttgttgcgg cgtgggactg ctgcctttaa tggcgtccgg
    12961 cacagtgaca caaccgctca gcattagcgc aaacgtacct gccagtatgc ctttgataac
    13021 attcttttga accgccatga tgcgactcct ttttttctca gggcatactc ttaagattca
    13081 ttctttgccc ggaagtttct tccatgcgac gttgttacgt aaataaactg gttcagcatg
    13141 ttccaccgcc accgttttac cctcggcaaa catctggcac gcaatcggca gcatatcttc
    13201 tgcagcgggc agtaacactt cgccatcgag caaaaccagc ccgctctctt taccgagatc
    13261 cggccaggct tgccagcccg tccctactgt cacccattcg ccggaaagct gttgcattcg
    13321 ttcatgaacg agttcgggtt tgagtacggc ttcggtttct tcaccgtgcc agataccgtt
    13381 ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa tagctgccag
    13441 cacgcgggtt gcgccgtttt tgcgccacgc cccttgcgcc atcgtcatta gcgtggagac
    13501 gccaatcatt ggtaactccg cgccaagtgc cagtccttgc gcaatgccaa taccaatgcg
    13561 cacgccggtg aagctaccgg ggccgcgccc gtaagccaga gcgtttatgt cagtcaggga
    13621 agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag tatgttcacg
    13681 agggcaaagc tcaaaatgag cgttgacagt accgtcgttc cacagggcga cagagcaggc
    13741 ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga tcaataaaaa
    13801 ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg caaggaaacg
    13861 aaccgcacgg gcaatgtcac gggtgcgcgg cgctggcggc agactggcga gaaacgtcgc
    13921 gccgtaaggg cgcatcacca gccgattgtc gcaaatcact aacacgccgc gatcgtcggc
    13981 gtcgcgaatc aatcgcccta ccccctgctt gagagtaatg accgcatccg gcagttgtac
    14041 ttcatcgaac ggatcaccac cgcgcaaacg gcaatcttcc atgcgtgctt ttaatagtgg
    14101 atcatccggc gaggtaaacg gcaatttgtc gataatcacc aatgacaatg tatcgccacg
    14161 cacgtccacc ccttcccaga aactgctggt tgccacaaga agcgcgttac cggcgctgac
    14221 aaattgctgc aacagttgcc ctttgctggt ttccccctgc aacaaaacgg gaagcgtcat
    14281 ggtagcgcgg aactgctcgg ccagatcgcg catcatggcg tgcgaggtac aaagcataaa
    14341 acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt gccgccagtt gccgtgcgga
    14401 acccggttga ttagtttgcg gcaggttgcg cggcacacag agtaacgcct ggcggctgta
    14461 atcaaagggg ctgggtaaca gcaacgactc tgccagttca atgccaagcc gcgaggtgaa
    14521 atgatgcaga tcgtcgttca ccgacagcgt tgctgaggta aagatccagc taccgggttt
    14581 ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag ccagagtaaa
    14641 atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct cttttagccg
    14701 tttcagccgt gtgcgatata acgtggcgcg ttcaaatgcc gcatccagca aggcggaacg
    14761 ccccagcgac agtttcgcca cgtcataaca aagttccaga gtgtcatcga gcagtaaaaa
    14821 tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat aacctggctc
    14881 accgagttgc aggcgaaaat cctgcgcact ctgggcaaga cggtcagcgc acttttgtaa
    14941 ctgctgggtg tcttttaatt cggtgcggta ggcgatggtg atgtcttttg ccaggtcgag
    15001 cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtcgg gcagctggtg
    15061 ggcttcgtcg aagatcatga cgtcagcttc cgggatcagt tcgccaaatc cactctcttt
    15121 caccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg cgtccatcgc
    15181 ttttttgcgt gctttgacta caaagcaatc tttatacatc gggcagtcgc tgccaaggca
    15241 gttatcgttg gtgctggtga ccagcggcca cgcctgtgaa tcttccgcca cgctgacgca
    15301 ggtgctgata tcaccatcga ctgtttgatt agaccaggag cgcagcagga tcacatcgct
    15361 taatatttgt accggcagat cgccgcccgc tagcgcctgt tgttcgagac gttcgaggca
    15421 gaggtagttt gagcgccctt tcagcaacgc cacgttgccc gtatatttca acgcctttga
    15481 aaccgttggc aaatcgcggc tgtagagctg atcctgcaac gcttttgaac cggtcgagat
    15541 aatgactttc tttttcgccc gcagcgcagg ggccaggtaa gcgtaggttt tgcccgtacc
    15601 ggttccggct tccaccacca gcggctggcc tttttctatc gcctgggtga cggctaccgc
    15661 catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca actgaccgtc
    15721 tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt atgtcaggat
    15781 gagggcgctt tcgccagttg aagtggtgac ggcgacctca cattgtggca gtctttgcag
    15841 cacgaaaatg gaaggataat gaggacaaaa tgatgactat cgttcgtatc gatgctgaag
    15901 cccgctggtc tgatgtggtg atccacaaca acacgctcta ctacactggt gtaccggaaa
    15961 acctcgacgc cgatgctttt gaccaaaccg ccaacacgct ggcgcagatt gacgccgtgc
    16021 tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc ctggctgata
    16081 aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg ggtcatgcgc
    16141 cggtgcgctg cacggtacaa gcgggtttga tgaacccgaa gtataaagtt gaaattaaga
    16201 ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat ttatcgacaa
    16261 tactggtcgg ttt
//
LOCUS       DADSZD010000101        16031 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.102, whole genome shotgun sequence.
ACCESSION   DADSZD010000101 DADSZD010000000
VERSION     DADSZD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.102"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            536..916
                     /gene="atpI"
                     /locus_tag="J1Z28_002942"
     CDS             536..916
                     /gene="atpI"
                     /locus_tag="J1Z28_002942"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBA7717758.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            925..1740
                     /gene="atpB"
                     /locus_tag="J1Z28_002943"
     CDS             925..1740
                     /gene="atpB"
                     /locus_tag="J1Z28_002943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBA7717759.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            1787..2026
                     /gene="atpE"
                     /locus_tag="J1Z28_002944"
     CDS             1787..2026
                     /gene="atpE"
                     /locus_tag="J1Z28_002944"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBA7717760.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            2088..2558
                     /gene="atpF"
                     /locus_tag="J1Z28_002945"
     CDS             2088..2558
                     /gene="atpF"
                     /locus_tag="J1Z28_002945"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBA7717761.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            2573..3106
                     /gene="atpH"
                     /locus_tag="J1Z28_002946"
     CDS             2573..3106
                     /gene="atpH"
                     /locus_tag="J1Z28_002946"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBA7717762.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            3119..4660
                     /gene="atpA"
                     /locus_tag="J1Z28_002947"
     CDS             3119..4660
                     /gene="atpA"
                     /locus_tag="J1Z28_002947"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBA7717763.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            4711..5574
                     /gene="atpG"
                     /locus_tag="J1Z28_002948"
     CDS             4711..5574
                     /gene="atpG"
                     /locus_tag="J1Z28_002948"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBA7717764.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            5601..6983
                     /gene="atpD"
                     /locus_tag="J1Z28_002949"
     CDS             5601..6983
                     /gene="atpD"
                     /locus_tag="J1Z28_002949"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBA7717765.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            7004..7423
                     /gene="atpC"
                     /locus_tag="J1Z28_002950"
     CDS             7004..7423
                     /gene="atpC"
                     /locus_tag="J1Z28_002950"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBA7717766.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            7776..9146
                     /gene="glmU"
                     /locus_tag="J1Z28_002951"
     CDS             7776..9146
                     /gene="glmU"
                     /locus_tag="J1Z28_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBA7717767.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            9307..11136
                     /gene="glmS"
                     /locus_tag="J1Z28_002952"
     CDS             9307..11136
                     /gene="glmS"
                     /locus_tag="J1Z28_002952"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBA7717768.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            11450..12490
                     /gene="pstS"
                     /locus_tag="J1Z28_002953"
     CDS             11450..12490
                     /gene="pstS"
                     /locus_tag="J1Z28_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBA7717769.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            12577..13536
                     /gene="pstC"
                     /locus_tag="J1Z28_002954"
     CDS             12577..13536
                     /gene="pstC"
                     /locus_tag="J1Z28_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBA7717770.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            13536..14426
                     /gene="pstA"
                     /locus_tag="J1Z28_002955"
     CDS             13536..14426
                     /gene="pstA"
                     /locus_tag="J1Z28_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBA7717771.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIASITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            14517..15290
                     /gene="pstB"
                     /locus_tag="J1Z28_002956"
     CDS             14517..15290
                     /gene="pstB"
                     /locus_tag="J1Z28_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBA7717772.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            15305..16030
                     /gene="phoU"
                     /locus_tag="J1Z28_002957"
     CDS             15305..16030
                     /gene="phoU"
                     /locus_tag="J1Z28_002957"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBA7717773.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
BASE COUNT         3840 a         3984 c         4341 g         3866 t
ORIGIN      
        1 tttaacgcca cgttcactct tttgtatcaa caagataacg tggctttttt tggtaagcag
       61 aaaataagtc ataagtgaaa atatcagtct gctaaaaatc ggcgctaaga atcatcattg
      121 actgttaaaa cattattaaa aatgtcaatg ggtggttttt gttatgtaaa tgtcatttat
      181 taaaacagta tctgttttta gactgaaata tcataaactt gcaaaggcat catttgccaa
      241 gtaaataaat atgctgtgcg cgaacatgcg caatatgtga tctgaagcac gctttatcac
      301 cagtgtttac gcgttattta cagtttttca tgatcgaaca gggtttgcag aaaagtcgca
      361 attgtatgca ctggaaaaat atttaaacat ttattcacct tttggctact tattgtttga
      421 aatcacgggg gcgcaccgta taatttgacc gctttttgat gcttgactct aagccttaaa
      481 gaaagtttta tacgacacgc ggcatacctc gaagggagca ggagtgaaaa acgtgatgtc
      541 tgtgtcgctc gtgagtcgaa acgttgctcg gaagcttctg ctcgttcagt tactggtggt
      601 gatagcaagt ggattgctgt tcagcctcaa agaccccttc tggggcgtct ctgcaataag
      661 cgggggcctg gcagtctttc tgcctaacgt tttgtttatg atatttgcct ggcgtcacca
      721 ggcgcataca ccagcgaaag gccgggtggc ctggacattc gcatttggcg aagctttcaa
      781 agttctggcg atgttggtgt tactggtggt ggcgttggcg gttttaaagg cggtattctt
      841 gccgctgatc gttacgtggg ttttggtgct ggtggttcag atactggcac cggctgtaat
      901 taacaacaaa gggtaaaagg catcatggct tcagaaaata tgacgccgca ggattacata
      961 ggacaccacc tgaataacct tcagctggac ctgcgtacat tctcgctggt ggatccacat
     1021 aaccccccag ccaccttctg gacaatcaat attgactcca tgttcttctc ggtggtgctg
     1081 ggtctgttgt tcctggtttt attccgtagt gtagccaaaa aggcaaccag cggcgtgcca
     1141 ggtaagtttc agaccgcgat tgagctggtg atcggctttg ttaatggtag cgtgaaagac
     1201 atgtaccatg gcaaaagcaa gctgattgct ccgctggcct tgacgatctt cgtctgggta
     1261 ttcctgatga acctgatgga tttactgcct atcgacctgc tgccgtacat tgctgaacat
     1321 gtactgggtc tgcctgcact gcgtgtggtt ccgtctgctg acgtgaacgt aacgctgtct
     1381 atggcactgg gcgtatttat cctgattctg ttctacagca tcaaaatgaa aggcatcggc
     1441 ggcttcacga aagagttgac gctgcagccg ttcaatcact gggcgttcat tcctgtcaac
     1501 ttaatccttg aaggggtaag cctgctgtcc aaaccagttt cactcggttt gcgactgttc
     1561 ggtaacatgt atgccggtga gctgattttc attctgattg ctggtctgtt gccgtggtgg
     1621 tcacagtgga tcctgaatgt gccgtgggcc attttccaca tcctgatcat tacgctgcaa
     1681 gccttcatct tcatggttct gacgatcgtc tatctgtcga tggcgtctga agaacattaa
     1741 tttaccaaca ctactacgtt ttaactgaaa caaactggag actgtcatgg aaaacctgaa
     1801 tatggatctg ctgtacatgg ctgccgctgt gatgatgggt ctggcggcaa tcggtgctgc
     1861 gatcggtatc ggcatcctcg ggggtaaatt cctggaaggc gcagcgcgtc aacctgatct
     1921 gattcctctg ctgcgtactc agttctttat cgttatgggt ctggtggatg ctatcccgat
     1981 gatcgctgta ggtctgggtc tgtacgtgat gttcgctgtc gcgtagtaag cgttgctttt
     2041 atttaaagag caatatcaga acgttaacta aatagaggca ttgtgctgtg aatcttaacg
     2101 caacaatcct cggccaggcc atcgcgtttg tcctgttcgt tctgttctgc atgaagtacg
     2161 tatggccgcc attaatggca gccatcgaaa aacgtcaaaa agaaattgct gacggccttg
     2221 cttccgcaga acgagcacat aaggaccttg accttgcaaa ggccagcgcg accgaccagc
     2281 tgaaaaaagc gaaagcggaa gcccaggtaa tcatcgagca ggcgaacaaa cgccgctcgc
     2341 agattctgga cgaagcgaaa gctgaggcag aacaggaacg tactaaaatc gtggcccagg
     2401 ctcaggcgga aattgaagcc gagcgtaaac gtgcccgtga agagctgcgt aagcaagttg
     2461 ctatcctggc tgttgctggc gccgagaaga tcatcgaacg ttccgtggat gaagctgcta
     2521 acagcgacat cgtggataaa cttgtcgctg aactgtaagg agggaggggc tgatgtctga
     2581 atttattacg gtagctcgcc cctacgccaa agcagctttt gactttgccg tcgaacacca
     2641 aagtgtagaa cgctggcagg acatgctggc gtttgccgcc gaggtaacca aaaacgaaca
     2701 aatggcagag cttctctctg gcgcgcttgc gccagaaacg ctcgccgagt cgtttatcgc
     2761 agtttgtggt gagcaactgg acgaaaacgg tcagaacctg attcgggtaa tggccgaaaa
     2821 tggtcgtctt aacgcgctcc cggatgttct ggagcagttt attcacctgc gtgctgtgag
     2881 tgaggctacc gctgaggtag acgtcatttc cgctgccgcg ttgagtgaac aacagctcgc
     2941 gaaaatttct gctgcgatgg aaaaacgtct gtcacgcaaa gttaagctga attgcaaaat
     3001 cgataagtct gtaatggcag gtgttatcat ccgagcgggt gatatggtca ttgatggcag
     3061 cgtacgcggt cgtcttgagc gccttgcaga cgtcttgcag tcttaagggg actggagcat
     3121 gcaactgaat tccaccgaaa tcagcgaact gatcaagcag cgcattgctc agttcaatgt
     3181 tgtgagtgaa gctcacaacg aaggtactat tgtttctgta agtgacggtg ttatccgcat
     3241 tcacggcctg gccgattgta tgcagggtga aatgatctcc ctgccgggta accgttacgc
     3301 tatcgcactg aacctcgagc gcgactctgt aggtgcggtt gttatggggc cgtacgctga
     3361 ccttgccgaa ggcatgaaag ttaagtgtac tggccgtatc ctggaagttc cggttggccg
     3421 tggcctgctg ggccgtgtgg ttaacactct gggtgcacca atcgacggta aaggtccgct
     3481 ggatcacgac ggcttctctg ctgtagaagc aatcgctccg ggcgttatcg aacgtcagtc
     3541 cgtagatcag ccggtacaga ccggttataa agccgttgac tccatgatcc caatcggtcg
     3601 tggtcagcgt gaattgatca tcggtgaccg tcagaccggt aaaaccgcac tggctatcga
     3661 tgccatcatc aaccagcgcg attccggtat caaatgtatc tatgtcgcta tcggccagaa
     3721 agcgtccacc atttctaacg tggtacgtaa actggaagag cacggcgcac tggctaacac
     3781 catcgttgtg gtagcaaccg cgtctgaatc cgcagcactg caatacctgg caccgtacgc
     3841 tggttgcgca atgggtgaat acttccgtga ccgcggtgaa gatgcgctga tcatttacga
     3901 tgacctgtct aaacaggctg ttgcttaccg tcagatttcc ctgctgctcc gtcgtccgcc
     3961 aggacgtgaa gcattcccgg gagacgtatt ctacctccac tctcgtctgc tggagcgtgc
     4021 tgcgcgtgtt aacgccgaat acgttgaagc tttcaccaaa ggtgaagtga aagggaaaac
     4081 cggttctctg actgcgctgc cgattatcga aactcaggcg ggtgacgttt ctgcgttcgt
     4141 tccgaccaac gtaatctcca ttaccgatgg tcagatcttc ctggaaacca acctgttcaa
     4201 cgccggtatt cgtcctgcgg ttaacccggg tatttccgta tcccgtgttg gtggtgcagc
     4261 acagaccaag atcatgaaaa aactgtccgg tggtatccgt accgctctgg cacagtatcg
     4321 tgaactggca gcgttctctc agtttgcatc cgaccttgac gatgcaacac gtaagcagct
     4381 tgaccacggt cagaaagtga ccgaactgct gaagcagaaa cagtatgcgc cgatgtctgt
     4441 tgcgcaacag tctctggttc tgttcgcagc agaacgtggt tacctggcgg atgttgaact
     4501 gtcgaaaatc ggcagcttcg aagccgctct gctggcttac gtcgaccgtg atcacgctcc
     4561 gttgatgcaa gagatcaacc agaccggtgg ctacaacgac gaaatcgaag gcaagctgaa
     4621 aggcatcctc gattccttca aagcaaccca atcctggtaa cgtctggcgg cttgccttag
     4681 ggcaggccgc aaggcattga ggagaagctc atggccggcg caaaagagat acgtagtaag
     4741 atcgcaagcg tccagaacac gcaaaagatc actaaagcga tggagatggt cgccgcttcc
     4801 aaaatgcgta aatcgcagga tcgcatggcg gccagccgtc cttatgcaga aaccatgcgc
     4861 aaagtgattg gtcaccttgc acacggtaat ctggaatata agcaccctta cctggaagac
     4921 cgcgacgtta aacgcgtggg ctacctggtg gtgtcgaccg accgtggttt gtgcggtggt
     4981 ttgaacatta acctgttcaa aaaactgctg gcggaaatga agacctggac cgacaaaggc
     5041 gttcaatgcg acctcgcaat gatcggctcg aaaggcgtgt cgttcttcaa ctccgtgggc
     5101 ggcaatgttg ttgcccaggt caccggcatg ggggataacc cttccctgtc cgaactgatc
     5161 ggtccggtaa aagtgatgtt gcaggcctac gacgaaggcc gtctggacaa gctttacatt
     5221 gtcagcaaca aatttattaa caccatgtct caggttccga ccatcagcca gctgctgccg
     5281 ttaccggcat cagatgatga tgatctgaaa cacaaatcct gggattacct gtacgaaccc
     5341 gatccgaagg cgttgctgga taccctgctg cgtcgttatg tcgaatctca ggtttatcag
     5401 ggcgtggttg aaaacctggc cagcgagcag gccgcccgta tggtggcgat gaaagccgcg
     5461 accgacaatg gcggcagcct gattaaagag ctgcagttgg tatacaacaa agctcgtcag
     5521 gccagcatta ctcaggaact caccgagatc gtctcggggg ccgccgcggt ttaaacaggt
     5581 tatttcgtag aggatttaag atggctactg gaaagattgt ccaggtaatc ggcgccgtag
     5641 ttgacgtcga attccctcag gatgccgtac cgcgcgtgta cgatgctctt gaggtgcaaa
     5701 atggtaatga gcgtctggtg ctggaagttc agcagcagct cggcggcggt atcgtgcgta
     5761 ccatcgcaat gggttcctcc gacggtctgc gtcgcggtct ggatgtaaaa gacctcgaac
     5821 acccgatcga agtcccggta ggtaaagcga ctctgggccg tatcatgaac gtactgggtg
     5881 aaccggtcga catgaaaggc gagatcggtg aagaagagcg ttgggcgatt caccgcgcag
     5941 caccttccta cgaagagctg tcaaactctc aggaactgct ggaaaccggt atcaaagtta
     6001 tcgacctgat gtgtccgttc gctaagggcg gtaaagttgg tctgttcggt ggtgcgggtg
     6061 taggtaaaac cgtaaacatg atggagctta ttcgtaacat cgcgatcgag cactccggtt
     6121 actctgtgtt tgcgggcgta ggtgaacgta ctcgtgaggg taacgacttc taccacgaaa
     6181 tgaccgactc caacgttatc gacaaagtat ccctggtgta tggccagatg aacgagccgc
     6241 cgggaaaccg tctgcgcgtt gctctgaccg gtctgaccat ggctgagaaa ttccgtgacg
     6301 aaggtcgtga cgttctgctg ttcgttgaca acatctatcg ttacaccctg gccggtacgg
     6361 aagtatccgc actgctgggc cgtatgcctt cagcggtagg ttatcagccg accctggcgg
     6421 aagagatggg cgttctgcag gaacgtatca cctccaccaa aactggttct atcacctccg
     6481 tacaggcggt atacgtacct gcggatgact tgactgaccc gtctccggca accacctttg
     6541 cgcaccttga cgcaaccgtg gtactgagcc gtcagatcgc gtctctgggt atctacccgg
     6601 ccgttgaccc gctggactcc accagccgtc agctggaccc gctggtggtt ggtcaggaac
     6661 actacgacac cgcgcgtggc gttcagtcca tcctgcaacg ttatcaggaa ctgaaagaca
     6721 tcatcgccat cctgggtatg gatgaactgt ctgaagaaga caaactggtg gtagcgcgtg
     6781 ctcgtaagat ccagcgcttc ctgtcccagc cgttcttcgt ggcagaagta ttcaccggtt
     6841 ctccgggtaa atacgtctcc ctgaaagaca ccatccgtgg ctttaaaggc atcatggaag
     6901 gcgaatacga tcacctgccg gagcaggcgt tctacatggt cggttccatc gaagaagctg
     6961 tggaaaaagc caaaaaactt taacgcctta atcggagggt gatatggcaa tgacttacca
     7021 cctggacgtc gtcagcgcag agcaacaaat gttctctggt ctggtcgaga aaatccaggt
     7081 aacgggtagc gaaggtgaac tggggatcta ccctggccac gcaccgctgc tcaccgccat
     7141 taagcctggt atgattcgca tcgtgaaaca gcacggtcac gaagagttta tctatctgtc
     7201 tggcggcatt cttgaagtgc agcctggcaa cgtgaccgtt ctggccgaca ccgcaattcg
     7261 cggccaggat ctcgacgaag cgcgagccat ggaagcgaaa cgtaaggctg aagagcacat
     7321 tagcagctct cacggcgacg tagattacgc tcaggcgtct gcggaactgg ccaaagcgat
     7381 cgcgcagctg cgcgttatcg agttgaccaa aaaagcgatg taacaccggc ttgaaaagca
     7441 caaaagccag tctggcaaca ggctggcttt ttttacgcgt attgacccgt cctgaaaagc
     7501 gttcacatag atcctgatga tataaaacac ccctgttttc ccgtttattc attgatcgaa
     7561 ataagagcaa aaacatccac ctgacgctta aatgaaggta ctgccttaat tttctgcaga
     7621 caaaaggcgt gacgatggtc gaaaatggcg ctttcgtcag cggggataat ccgttattga
     7681 acaatttatc ctctgtccat ttcacgatga aaaaaatgta gttttttcaa ggtgaagcgg
     7741 tttaaattcg ttctcaaatt acagtcagga cgcgtatgtt gaataatgct atgagcgtag
     7801 tgatccttgc cgcaggcaaa ggcacgcgca tgtattccga tcttccgaaa gtgctgcata
     7861 cccttgccgg gaaagcgatg gttcagcatg tcattgatgc tgcgaatgaa ttaggcgcag
     7921 cgcacgttca ccttgtgtat ggtcacggcg gcgatctgct taagcaggcg ctgaaagacg
     7981 acaacctgaa ctgggtgctt caggcagaac agctgggtac gggtcatgcg atgcagcagg
     8041 ccgcaccttt ctttgccgat gatgaagaca ttttaatgct ctacggcgac gtgccgctga
     8101 tctctgtcga aacactccag cgtctgcgtg atgctaaacc gcagggtggc attggtctgc
     8161 tgacggtaaa actggatgat ccgaccggtt atggacgtat cacccgtgaa aacggcaaag
     8221 ttaccggcat tgttgagcac aaagatgcca ccgacgagca gcgtcagatt caggagatca
     8281 acaccggcat tctgattgcc aacggcgcag atatgaaacg ctggctggcg aagctgacca
     8341 acaataatgc tcagggcgaa tactacatca ccgacattat tgcgctggcg tatcaggaag
     8401 gacgtgaaat cgtcgccgtt catccgcaac gtttaagcga agtagaaggc gtgaataacc
     8461 gcctgcaact ctcccgactg gagcgcgttt accagtccga acaggctgaa aaactgctgt
     8521 tagcaggcgt tatgctgcgc gatccggcgc gttttgatct gcgcggtacg cttactcacg
     8581 ggcgcgatgt tgaaattgat actaacgtta tcatcgaggg caacgtgact ctcggtcatc
     8641 gcgtgaaaat cggcaccggt tgcgtgatta aaaacagcgt gattggcgat gattgcgaaa
     8701 tcagtccata taccgtcgtg gaagacgcga atctggcagc ggcctgtact attggcccgt
     8761 ttgcccgtct gcgtcctggt gctgagttgc tggaaggtgc acacgtcggt aacttcgttg
     8821 agatgaaaaa agcgcgtctg ggtaaaggct cgaaagctgg tcatctgact tacctgggcg
     8881 atgcggaaat tggcgataac gttaacatcg gcgcgggaac cattacctgc aactacgatg
     8941 gtgcgaataa atttaagacc attatcggcg acgatgtgtt tgtcggttcc gacactcaac
     9001 tggtggcccc ggtaacagta ggcaaaggcg cgaccattgc tgcgggtaca actgtgacgc
     9061 gtaatgtcgg cgaaaatgcc ctggcgatca gccgtgtgcc gcagactcaa aaagaaggct
     9121 ggcgtcgtcc ggtaaagaaa aagtaattct ggccgggtaa tccggtcata tgggatgagg
     9181 agataacata atctcccgcc cacaagcagt aactataaaa ataaccccac tctctacaag
     9241 gctcggggcg cccgaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca
     9301 aaaactatgt gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt
     9361 gaaggtttac gtcgtctgga ataccgcgga tatgattctg ccggtctggc cgttgttgat
     9421 gcagaaggtc atatgacccg cctgcgtcgc ctcggtaaag tacagatgct ggctcaggca
     9481 gcggaagaac atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac
     9541 ggtgaacctt cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat
     9601 aacggcatca tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc
     9661 ttcgtttctg aaaccgacac cgaagtgatt gcacatttgg tgaactggga gctgaaacaa
     9721 ggcggtactc tgcgtgaggc agttctgcgt gctatcccgc aactgcgtgg tgcgtacggt
     9781 acagtgatca tggactcccg tcatccggat accctgctgg cggcacgttc tggtagtccg
     9841 ctggtgattg gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg
     9901 ccagtgactc gtcgctttat cttccttgaa gagggcgata ttgcggaaat aactcgccgt
     9961 tcggtaaaca tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat
    10021 ctgcaatatg acgcgggcga taaaggtatt taccgccact acatgcagaa agagatctac
    10081 gaacagccga acgcgatcaa aaacaccctt accgggcgca tcagccacgg tcaggttgat
    10141 ttaagcgagc tgggaccgaa cgccgatgaa ctgctgtcga aggttgagca tattcagatc
    10201 ctcgcctgtg gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg
    10261 gcaggtattc cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgcaa atctgccgtg
    10321 cgtcgtaaca gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg
    10381 ggcctgcgtc tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg
    10441 ggttcttctc tggtgcgcga atccgatctg gcgctgatga ccaacgccgg tacggaaatt
    10501 ggcgtggcct ccaccaaagc attcaccacc cagttaactg tgctgttgat gctggtggcg
    10561 aaactgtctc gcctgaaagg tctggatgcc tccattgaac atgacatcgt gcatggtctg
    10621 caggcgctgc cgagccgtat tgagcagatg ctgtctcagg acaaacgcat tgaagcgctg
    10681 gcagaagatt tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca
    10741 atcgcgctgg aaggtgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac
    10801 gctgcaggcg aactgaaaca cggtccgctg gcgctgattg atgccgatat gccggttatc
    10861 gtcgttgcac cgaacaacga attgctggaa aaactgaaat ccaacattga agaagttcgc
    10921 gcgcgtggcg gtcagttgta tgtcttcgcc gatcaggatg cgggttttgt gagcagtgac
    10981 aatatgcaca ttattgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc
    11041 gttccgctgc aactactggc ttaccacgtt gcgctgatca aaggcaccga cgttgaccag
    11101 ccgcgtaacc tggcaaaatc ggtaacggtt gagtaataaa tggatgccct gcgtaagcgg
    11161 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc
    11221 gtcatcttcg gctacttttt ctctgtcaca gaatgaaaat ttttctgtca tctcttcgtt
    11281 attaatattt gtaattaact gaatatcaac gcttatttaa atcagactaa agactttctc
    11341 tctctgtcat aaaactgtca taaaccttac atataactgt cacctgtttg tcctattttg
    11401 cttctcgtag ccaacaaaca atgctttatg aatcctccca ggagacatta tgaaagttat
    11461 gcgtaccacc gtcgcaactg ttgtcgccgc gaccttatcg atgagtgctt tctctgtgtt
    11521 tgcagaagca agcctgacag gtgcaggtgc aaccttccct gcgccggtgt atgccaaatg
    11581 ggctgacact taccagaaag aaaccggtaa taaagttaac taccagggta tcggttcttc
    11641 cggtggcgta aaacagatta tcgctaatac cgttgatttc ggtgcttctg atgcgccgtt
    11701 atctgacgaa aaactggctc aggaaggcct gttccagttc ccgaccgtga ttggcggcgt
    11761 ggtgctggcg gttaatattc caggtctgaa gtctggcgaa ctggtgctgg atggtaaaac
    11821 cctcggcgac atctacctgg gcaaaatcaa gaagtgggat gatgaagcca tcgccaaact
    11881 gaatccgggt ctgaaactgc cttcacagaa cattgctgta gtacgccgcg cagatggctc
    11941 cgggacttcc ttcgtcttca ccagctacct ggcgaaagtg aacgaagagt ggaaaaacaa
    12001 cgttggtact ggctctaccg taaaatggcc gatcggtctg ggcggtaaag gtaacgacgg
    12061 tatcgccgcg ttcgttcagc gtctgccggg tgcaattggt tacgttgaat atgcttacgc
    12121 gaagcagaac aacctggcgt acaccaaact gatctccgct gatggcaaac cggtgagtcc
    12181 gaccgaagaa aacttcgcta atgcagcaaa aggcgcagac tggagcaaaa cattcgctca
    12241 ggatctgacc aaccagaaag gcgaagatgc atggcctatt acctctacca cgttcattct
    12301 gatccataaa gatcagaaga aaccagaaca aggcaccgaa gtgctgaagt tcttcgactg
    12361 ggcatacaaa accggggcta aacaggcgaa cgacctggat tatgccagcc tgccggatag
    12421 cgtagttgaa caggttcgcg ctgcgtggaa gaccaatatt aaagacagta gcggcaagcc
    12481 gctgtactaa taaaactcca ggccgggtac ggtgttttac gccgcatccg gcattacaaa
    12541 atgactttgt aaacgcgttt aactgaagag taacttatgg ctgcaaccaa gcctgctttt
    12601 aacccaccgg gtaaaaaggg cgacataatt ttcagcgtgc tggtaaaact ggcggcgctg
    12661 attgtgctat tgatgttggg tggcattatt gtctctctga tcatctcatc ctggccgagc
    12721 attcagaaat ttggtctggc tttcctgtgg accaaagagt gggatgcgcc gaacgatatc
    12781 tacggggcgc tggtgccgat ctacggtacg ctggtgacct cgtttatcgc gctgctgatc
    12841 gccgtcccgg tgagttttgg tatcgccctg ttcctgaccg agcttgcgcc tggctggctg
    12901 aaacgcccgc tgggtatcgc cattgagctg ctggctgcca ttccaagtat cgtttacggc
    12961 atgtggggcc tgtttatctt tgcgccgctg ttcgccgttt acttccagga gccggtcggc
    13021 aatatcatgt cgaatatccc gattgtcggc gcgctgttct ccggcccggc ctttggtatc
    13081 ggtatcctcg cggcaggcgt gatcctcgcc atcatgatta ttccgtacat tgcagcggta
    13141 atgcgcgatg tattcgaaca aaccccggtg atgatgaaag agtcggccta cggtattggc
    13201 tgcaccacct gggaagttat ctggcgtatc gttcttccgt tcaccaaaaa tggtgttatc
    13261 ggcggcatta tgttggggct ggggcgcgcg ctcggtgaaa ccatggcggt gacctttatc
    13321 atcggtaaca cctaccaact cgacagcgcc tcgctgtata tgccgggcaa cagtatcacc
    13381 tctgcgctgg ccaacgaatt tgcggaagcg gaatccggtc tgcacgtcgc tgcactgatg
    13441 gaactgggcc tgatcctgtt tgtgattacc ttcatcgtcc tcgccgcatc gaagtttatg
    13501 attatgcgcc tggctaagaa tgagggggca cgctaatggc tatggttgaa atgcaaacca
    13561 ctgcggcact ggctgaatcc cgccgcaaaa tgcaggcgcg tcgccgcctc aaaaaccgta
    13621 ttgcgctgac gctctcaatg gcgacgatgg ccttcggtct gttctggctg atctggattt
    13681 taatgtccac catcactcgc ggtatcgacg gtatgtcgct ggcgctgttc accgaaatga
    13741 cgccaccacc caatacggaa ggcggtggtc tggcgaacgc cctggcgggt agcggcctgt
    13801 taattttgtg ggctaccgta ttcggtacgc cgctgggcat tatggcgggg atttacctgg
    13861 ctgaatatgg tcgtaaatcc tggctggcag aagtgattcg cttcattaac gacattctgc
    13921 tctctgcgcc gtcgattgtg gttggtctgt ttgtttacac cattgtggtg gcgcagatgg
    13981 agcacttctc cggctgggcg ggcgtgattg cgctggcgct gttgcaggtg ccgattgtta
    14041 tccgcaccac cgagaacatg ctgaaactgg tgccggacag cctgcgtgaa gcggcttatg
    14101 cgctgggtac gccgaagtgg aagatgatcg cctcgatcac gctgaaagcg tcggtgtccg
    14161 ggatcatgac cggtatcctg ctggcgattg cacgtattgc tggtgaaacc gcgccgctgc
    14221 tgtttactgc gctctccaac cagttctgga gcacggacat gatgcagccg atcgccaacc
    14281 tgccggtgac gatctttaag tttgcgatga gcccgtttgc ggaatggcag caactggcct
    14341 gggccggggt gttgatcatt accctgtgcg tactgctgct gaacattctg gcgcgcgttg
    14401 tttttgcgaa gaataaacac ggttaacact ttgctggatg cggcgtaaac gccttatccg
    14461 gcctacggta agcctgattc gcgaagtgca tcaggcacga tgaggaaaag attgcaatga
    14521 gtatggttga aactgccccg agtaaaattc aggttcgtaa tttgaacttc tactacggca
    14581 aattccatgc cctgaaaaac atcaacctgg atatcgccaa aaaccaggtt acggcgttta
    14641 tcgggccgtc cggctgcggt aaatcaacgc tgctgcgtac cttcaacaaa atgtttgaac
    14701 tgtacccgga acagcgtgcg gaaggtgaaa ttctgctcga tggcgacaac atcctgacca
    14761 actctcagga tatcgccctg ctgcgtgcga aagtgggcat ggtgttccag aaaccgacgc
    14821 cgttcccaat gtccatctac gacaacatcg cttttggcgt gcgtctgttt gagaagctct
    14881 cccgtgctga catggacgag cgcgtgcagt gggcattgac caaagccgca ttgtggaacg
    14941 aaaccaaaga taaattgcac cagagcggtt actctctctc tggtggtcag caacagcgtc
    15001 tgtgcattgc gcgtggtatc gccattcgcc cggaagtgct gctgctcgac gaaccgtgtt
    15061 cggcactcga ccctatctct accgggcgta ttgaagagct gatcaccgaa ctgaagcagg
    15121 attacaccgt ggtgatcgtc acccacaaca tgcagcaggc tgcgcgttgt tccgaccaca
    15181 cggcgtttat gtacctgggc gaattgattg agttcagcaa cacggacgat ctgttcacca
    15241 agccagcgaa gaaacaaaca gaagactaca tcaccggtcg ttacggttga ttcaggagtg
    15301 cgttatggac agtctcaatc ttaataaaca tatttccggc cagttcaacg ccgaactgga
    15361 aagcatccgc acgcaggtga tgaccatggg cggcatggtg gagcagcagc tttctgatgc
    15421 aatcaccgcg atgcataacc aggacagcga tctggcgaag cgcgtcatcg aaggcgacaa
    15481 gaacgtcaac atgatggaag tggcgatcga tgaagcctgc gtgcgcatta tcgccaaacg
    15541 tcagccgacg gcgagcgacc tgcgtctggt tatggtgatc agtaaaacca ttgccgagct
    15601 ggagcgtatt ggcgacgtgg cggacaaaat ctgccgtact gcactggaga aattctccca
    15661 gcagcatcag ccattgctgg taagtctgga gtcgctgggc cgtcatacca tccagatgct
    15721 gcacgacgtg ctggacgcgt tcgcgcggat ggacattgac gaagcggtac gtatttatcg
    15781 tgaagataaa aaagtcgatc aggagtacga aggtattgtt cgtcaactga tgacctacat
    15841 gatggaagat tcgcgtacca ttccgagcgt acttaccgcg ttattctgtg cgcgttctat
    15901 cgaacgtatt ggcgaccgct gccagaatat ttgtgagttt atcttctact acgtgaaggg
    15961 gcaggatttc cgtcatgtcg gtggtgacga gctcgataaa ctgctggcag aaaaagatag
    16021 cgataaataa t
//
LOCUS       DADSZD010000102        15599 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.103, whole genome shotgun sequence.
ACCESSION   DADSZD010000102 DADSZD010000000
VERSION     DADSZD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.103"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            303..1574
                     /locus_tag="J1Z28_002958"
     CDS             303..1574
                     /locus_tag="J1Z28_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBA7717774.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSLVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            complement(1604..2608)
                     /gene="dppF"
                     /locus_tag="J1Z28_002959"
     CDS             complement(1604..2608)
                     /gene="dppF"
                     /locus_tag="J1Z28_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBA7717775.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRQEKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2605..3588)
                     /gene="dppD"
                     /locus_tag="J1Z28_002960"
     CDS             complement(2605..3588)
                     /gene="dppD"
                     /locus_tag="J1Z28_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBA7717776.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3599..4501)
                     /gene="dppC"
                     /locus_tag="J1Z28_002961"
     CDS             complement(3599..4501)
                     /gene="dppC"
                     /locus_tag="J1Z28_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBA7717777.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4511..5530)
                     /gene="dppB"
                     /locus_tag="J1Z28_002962"
     CDS             complement(4511..5530)
                     /gene="dppB"
                     /locus_tag="J1Z28_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBA7717778.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(5681..7288)
                     /gene="dppA"
                     /locus_tag="J1Z28_002963"
     CDS             complement(5681..7288)
                     /gene="dppA"
                     /locus_tag="J1Z28_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBA7717779.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(8032..8108)
                     /locus_tag="J1Z28_002964"
     tRNA            complement(8032..8108)
                     /locus_tag="J1Z28_002964"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8072..8074),aa:Pro,seq:cgg)
     gene            complement(8200..9891)
                     /gene="eptB"
                     /locus_tag="J1Z28_002965"
     CDS             complement(8200..9891)
                     /gene="eptB"
                     /locus_tag="J1Z28_002965"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBA7717780.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            complement(10144..10668)
                     /gene="lpfE"
                     /locus_tag="J1Z28_002966"
     CDS             complement(10144..10668)
                     /gene="lpfE"
                     /locus_tag="J1Z28_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HBA7717781.1"
                     /translation="MKRTILALCLLPFSFSSLSATTDLGAKGTLKFSLKISQGACELV
                     KDSVEVDMGLAVLKKPVRIGTELNPTTFSIGLKNCSEVVRAYVTMDGTPNADDPNLFA
                     LDAGGATGVGLKIKTSAGVQQFPKNTTPTPIEFAINGDGAHQLNYIASYVPLRADATI
                     GRADATVDFTVQYE"
     gene            complement(10674..11726)
                     /gene="lpfD"
                     /locus_tag="J1Z28_002967"
     CDS             complement(10674..11726)
                     /gene="lpfD"
                     /locus_tag="J1Z28_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HBA7717782.1"
                     /translation="MKIKNLLAGMVLLGTSTFTGNIWAADWGPCRTASGDPFIFVTSF
                     TKNIQNPTDNVTGQTYPDFYQWALGDKYSGVCECPSPNPTEARPTLYKTESTLAAGHN
                     STYFKITNNLEVSTRVYIANVGNVQVPFINKSNSQPGRECDQPTFGWTTGSKGQLSLY
                     IAKPFVGEQNIPQTIIVSVFGTKKENVYSSVPISQVLLSGKVTVTQGCELAAGTSLDI
                     DFGEYQAHDFKGRTGQPPQNVQKIQKELTFNCTNISDGVHIYLSLEGTPNAAYPSAIS
                     LGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEYVKRKVTTTITAYPVSTTGKLP
                     AAGDYSGVATMHVELD"
     gene            complement(11737..14268)
                     /gene="lpfC"
                     /locus_tag="J1Z28_002968"
     CDS             complement(11737..14268)
                     /gene="lpfC"
                     /locus_tag="J1Z28_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HBA7717783.1"
                     /translation="MHWMHLPLDHYRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHVDLSVYDSPVGQQIPGKYRVFVFVNEEKMASRTLDFSTASEAQRKASGESLMP
                     CLSRVQLEEMGVRIDSFPALKILPPEACVAFDEIIPQATSRFDFNTQTLHLTFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGSGSSPDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKESDGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSGKPRFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKDEG
                     QSYRFLYSKSFEGGTELRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSIAYTWTRSPEWDED
                     DRLWSFSLSIPLGGAWGSYRMTTDQNGKTSQQASVSGTLLEDRNLNYNVQQGYTSNGV
                     GNSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGAHGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAIVRSRFDTRVGYRVLMSLKRANGNAVPFGATAALSDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGHEQAQRCRVPFRLPEKKDNSGIVMVNAVCDK"
     gene            complement(14291..14977)
                     /gene="lpfB"
                     /locus_tag="J1Z28_002969"
     CDS             complement(14291..14977)
                     /gene="lpfB"
                     /locus_tag="J1Z28_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HBA7717784.1"
                     /translation="MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGV
                     AGEMLTKSIN"
     gene            complement(15061..15585)
                     /gene="lpfA"
                     /locus_tag="J1Z28_002970"
     CDS             complement(15061..15585)
                     /gene="lpfA"
                     /locus_tag="J1Z28_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HBA7717785.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGSVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTTTGDKSAATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     ISVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVATKNAVTI
                     GYGNAEVDFNLTYN"
BASE COUNT         3833 a         4073 c         3725 g         3968 t
ORIGIN      
        1 cccttccggc ttgccgctga ttttacgatt gttttttgcc gtcagcaccc ggcaatcttc
       61 ctcattgcgt gatgccttcc agcttaattt ctttatttgc aatagttgca gctcatatcc
      121 gggctgactc cagactagta gtgaaatgcg caattttgta gaattgcgcc tcctcgcgac
      181 ctccccagtg aatctgttgc ctcatttttt acatactgcg tattcgactt ctccacctgt
      241 tgcgcaagag aaactgggtt tattcatttt tgcgaggccg acttctttct ggacaggact
      301 ttatgcagca caacacacta ccgaaacacg atcagaaatt gccgtttaca cgctacgact
      361 tcggctgggt tttattatgc ataggcatgg cgattggtgc cggaaccgtg ctgatgccag
      421 tacaaattgg cttgaaggga atttgggtat ttattaccgc agcgatcatt gcttatcctg
      481 ccacctgggt agtacaggac atttatttaa aaaccctttc tgaaagcgat tcctgtaatg
      541 actacaccga tattatcagt cattacctgg ggaagaactg gggaattttc ctcggggtta
      601 tctacttttt gatgattatc cacgggattt ttatctactc tctctccgtg gttttcgaca
      661 gcgcctcgta cctgaaaacc ttcggtttaa ccgatgccga tctttctcaa tctctatttt
      721 ataaagtcgc cattttcgcc gtactggtgg cgattgcgtc tggtggtgaa cgattactgt
      781 ttaagatttc cggaccaatg gtggtggtca aagtggggat tattgtcgtg ttcggttttg
      841 cgatgattcc gcactggaat ttcgccaata taaccgcctt cccgcaagcc tccgactttt
      901 tccgcgatgt cctgcttact attccatttt gcttcttttc cgcggtattt attcaggtac
      961 ttaacccaat gaatattgcc tatcgtaaac gggaagcgga taaagtactg gcaacccggc
     1021 tcgcgctgcg tacccaccgg attagctata tcacgctcat tgcggtgatc ttgttttttg
     1081 ccttttcgtt taccttctca attagccacg aagaagccgt ttctgccttt gaacaaaata
     1141 tctcggcact ggcactggcc gcgcaggtga tccctgggca tatcattcat atcacctcta
     1201 cggtactgaa tatctttgcc gtactgactg cattctttgg catttatctc ggtttccacg
     1261 aggccattaa aggcattatt ctcaatctgt taagccgaat tattgatacc aggaaaatta
     1321 actcactcgt gctgactctg gcgatctgca cttttatcgt cattacgttg acgatttggg
     1381 tttcgtttcg tgtatcggta ctggtgttct ttcagttggg aagcccgtta tatggcattg
     1441 tgtcgtgcct cattccgttt ttcctgatct ataaagtctc acaactggaa aaacttcgcg
     1501 ggtttaaagc ctggatgatt ctgctgtacg gcattttgtt atgcttgtcg ccactgttga
     1561 agttgattga gtaaaccgga gcgcatggcc ccggttttgt gagttaacgc tgcggatttt
     1621 catcctgatc aacggcaaaa caggctacca gttgaccgcc gtagtctttt agctgcggct
     1681 gcaactggat gcaggggccg aagcgccgac gacagcgggc gttgaaggcg cagcctggcg
     1741 gtggattcag cgggcttggc agttcaccgg taagtttgat gcgttcgcga cgatcgtccg
     1801 ggttcaggcg tggtgtcgcg gagagcaatg cctgagtgta cgggtggcgc gggttattga
     1861 agatttggtc cttcgtcccc ttctccacgc agcggcccag gtacatcacc atcacttcat
     1921 cagcaatgtg ctccaccacc gacagatcgt gcgagataaa gacataagat agccccagtt
     1981 cctgctgcaa atccatcatc agattcagca cctgcgcacg cactgagacg tccagcgcag
     2041 aaaccggctc atcggcaatc accacatccg ggtcgagcat cagacctcgg gcaatggcga
     2101 tacgctgacg ctgaccgccg gagaacatat gcggatagcg gtcgtaatgc tcggttttca
     2161 ggccgacttt cgccatcatc gacagggctt tttcctgacg ctgttctttg cttaagctgg
     2221 tgttgatcag cagcggctct tcgagaatct gcccaacttt tttacgcgga ttcagcgaac
     2281 cgtaagggtt ctggaagaca atctggattt tctgccgacg tagcttctgc gcctgcggat
     2341 cgtgcttgag cagatcctgc ccctggtaat acagttcgcc gccggtgggc gtttcaatca
     2401 tcgtcagcaa ccgaccgagg gtcgatttac cgcagccaga ttcacctact actgccagcg
     2461 ttttgccacg ttcaaggtta aacgaaacgc catccagcgc cttcaccaga cgttccggcg
     2521 cgaacatgcc tttcttcacc ggataatgtt ttttcaggtc gatagcctgc aacagcggtt
     2581 gttgcgaggt ggcctcatgc gtactcatag tgtcggcctc ccggcatcat caagtgggta
     2641 atggcatttg gactgacgcc cgtcagcgag catattcagc gccggttctt cagcacggca
     2701 tttgtcagtg gcgtaggggc agcgcgggtt aagcaggcag ccgttcgggc ggtcgtactt
     2761 gccgggaacg acacctggca acgacgccag acgttctttg tcctgagcaa attccggcag
     2821 cgcacgcagc aatgcctgag tatacgggtg acgcggcgca tggaagatag cgtgcgcatc
     2881 gcccgtttct actacctggc ctgcatacat cacgatgatt ttatgtgccg cttccgccac
     2941 cagcgccagg tcatgagtga tcaacaccag cgccatgttc tctttctgct gcaactccag
     3001 cagcagttcg atgatttgcg cctggatggt cacgtccagc gcagtggtcg gttcatcggc
     3061 aatcagcagt tttggtcgac aggcaatcgc catggcgatc atcacgcgct ggctcatgcc
     3121 gccggaaagc tgatgcgggt aaacatccag acgcgatgcc ggatcgggaa taccgaccag
     3181 attcagcagg tcaatcgctc gctgacggcg ggtacttttg ttgccgccct gatgcacctt
     3241 aatcgcttcc ataatctgga aacccacggt gtagcacggg ttaaggctgg tcatcgggtc
     3301 ctggaagatc attgccactt cggcacccac caggttgcgg cgctcttttt ctgagatacg
     3361 ctgcaaatcc tgaccgttaa actccagctt ttccgccatt acgcggcccg gataatcaat
     3421 cagccccata attgccagcg aactgaccga tttaccagag ccggactcac ccacaatccc
     3481 gaccacttca ccctgtttta cgctgtagct gatgcggtct acggcgcgga acggtgcgct
     3541 ttcgtcgccg aaatgcaccg ataatttatc tacatttaat aacgccatct cgaacctctt
     3601 actgcttcag tttgggatcg agcgcgtcac gcagaccgtc acccatcagg ttaaatgcca
     3661 gcaccgtcag caggatcgcc agacccggga aggtcacgac ccaccaggcg ctttgcgcga
     3721 actgcaacac gtcagagagc atggtgcccc actccggcgt tggcggctgc gcgcccatgc
     3781 cgaggaaacc aagagcagcc atatcgagaa tggcgttaga gaaaccgagc gacgcctgaa
     3841 caatcagcgg cgcaaggcag ttcgggaaga tgttaataaa catctggcgc attgccccgg
     3901 cacccgccac gcgagacgcg gtgacgtaat cgcggttaac ttccaccagc acggcggcgc
     3961 gggttaagcg cacataatgc ggcagcgcaa cgaaggttag cgccagcgcg gcgttaccaa
     4021 tcgacgggcc aaaaatcgcc accagcacca gtgccagcag tagacttggc agcgccagca
     4081 tgatatcgac cacgcgcatg atgatgttat cgaccagacc gccaaagtaa ccagcgatca
     4141 ggccgagaat aacgcccata atcagcgata acaccactac cagacagcca accagcagcg
     4201 acaagcgcgc gccgtacatc agccgcgaca gcacatcacg ccccacgtca tcagtgccca
     4261 gcaagtgcgc catgctgccg ccttcctgcc aggctggcgg ggcgagcagt gcatcgcgga
     4321 actgttccgc tgggttatag ggtgcaatcc agttggcaaa gatcgcgata aacagcacga
     4381 tgacgacgta aaccagcccg acaaccgcgc ctttgttgcg tttaaaatag tgccagaact
     4441 cctgtaacgg ggtcatcggc accggtgcgc taatcacttt attttcagta acctgtgaca
     4501 tgatgtcccc ttacttctta tgacgaatac gcgggttcac cacgccgtac agcagatcga
     4561 ccagcaggtt gacgaggata atcatcgtcg ccaccagcaa tacgccgccc tgcactaccg
     4621 gataatcgcg gcgttgcagt gcgtcaatca accagcgccc cagaccgggc cacgagaaga
     4681 tggtttcagt cagaatcgcc cccgccagca atgttcccac ctgcaggccg ataacggtca
     4741 ccaccggcag catcgcgtta cgcagcgcat ggacgataat cacccgcatc cgggtaagac
     4801 ctttggcacg cgcggtgcgg atataatctt cgcccagcac ttccagcatc gaggagcgcg
     4861 tcatacgcac aatgaccgcc agcggaatag tacccagcac aatggcgggc aggatcatat
     4921 gggcgacggc atcgataaag ttaccgtctt caccccagat ggcggtgtcg atcagcataa
     4981 aaccggttaa cggattggaa tcatcgagga acaccatgtc gctcacacga ccggagacag
     5041 gcgtcaggtt ccagtgcacc gaaaccagca tgatcagcat catgccccac cagaaaattg
     5101 gcatcgagta gccggtcagc gccaggccaa ccgctgtgtg atcgaaaatg gaaccacgtt
     5161 taaccgcagc cagcacacca accggaatcc cgactgccgt cgcaaaaatc atcgcgcaga
     5221 cgccaagttc cagcgtggcc tgaaagcgcg gcacgaactc ttcccatacc ggaattcggc
     5281 ttttcatgga aatgcctaaa tcaccgtgca ttacgcccca aatgtaatgg agatactgct
     5341 gccacatcgg tttatctaag ccgagttcag ccagcaattg cgcgtgacgc tctggggaga
     5401 tcccacgttc gcccgccatg atcatcaccg gatcgcccgg gatcatgtgg acaaaggcaa
     5461 atgtgagaag ggtaataccg ataaacgtgg ggatgacgag tcccaaacgt cggagaataa
     5521 actgcaacat aacccggatt ctctatagtg acccgcagcc tggaactgcg tgtctgtatt
     5581 gctcacaaat caattcccgc ccttgtggga cagggaatga agtattgctg ccggatggcg
     5641 ctgcacgcag aggcacagcg ccaccgggta tggcttttaa ttattcgata gagacgtttt
     5701 cgaagtgatg tttgcctaat ggatcaacca catagccttt aacttctttg cgtaccggtt
     5761 caaacacggt ggagtgagcg atgatcagtg ccggagcctg atcgtgcatc acgacctgtg
     5821 cttgtttgta cagttcaaca cgtttattgt ggtcgtcggt agcacgcgcc ggttgaatca
     5881 gatcttcaaa cggtttgtag caccattttg agtagttgga gccttgttca gaggcggcgc
     5941 agctgaacag ggtggcgaag aagttatccg gatccccgtt atcgccagtc cagcccatca
     6001 ttaccgtctg atgctcgcca tctttcgcac gcttgaggta ctcaccccat tcgtaggtga
     6061 cgattttggc ctgcacgccc actttcgccc agtctgcctg aatcatctcc gccatgcggc
     6121 gagcattcgg gttatacgga cgttgtaccg gcattgccca caggtcgatg gagaaacctt
     6181 tttccagacc cgcttctttc agcaaggctt tcgctttttc aggatcgtaa gtgtagtcct
     6241 gaacgtcgtc gttatagccc cacatggttg gcgggatcag gtttttcgct gatacgcccg
     6301 cgccctgata aaccgctttg atgatggcgt ctttgttcac cgcgtaggtc agagcctggc
     6361 gaactttcac gtcatccagt ggttttttct gcacgttata cgagagataa ccgacgttca
     6421 gccccggcat ttccatcagg ttgatggatt tatcctgctt catgcgggcg atatctgccg
     6481 ggttcgggta cggcatcacc tggcattcgt tcttctgcaa tttcgcgtaa cgcacggaag
     6541 cgtcaggagt aatagagaag accagcgtat cgatcttcgg tttggtgccc cagtagccat
     6601 caaacgcttt atagcgaata cgggaatctt tctggtactg ctgtaactgg aacgggccgg
     6661 taccgattgg gttgaggtcc agtttttccg gtgtaccggc tttcatcatc gcgtcagcat
     6721 attcttttga cagaatagag gcgaagtcca ttgccaggtc agcgaggaac ggcgcttccg
     6781 ggcgagtcag cacaaactga acggtgttat cgtccacctt tttcacttcg ctgatcagct
     6841 ctggtaagcc catgccttcg aagtattcgt agctgccgcc agagacttta tggtacgggt
     6901 tttgcgcgtt tttctgacga tcgaacgaga acactacgtc atcggcattc agttcgcgcg
     6961 tcggtttgaa ttctttattg tcgtgccact tcacaccttt acgcagatgg aaggtatagg
     7021 ttttaccgtc ttcgctgact tcccactttt cagcgaggcc cgggatcact tcagtggtgc
     7081 cgattttaaa ttcaaccaga cggttataaa gcggtacgga agaggcgtca taggtggtgc
     7141 cggaggtaaa cagctgcggg ttaaaccctt ccggagatcc ttctgagcaa taaaccagag
     7201 ttttagcctg aacacttgct gcgacggtca tagccaccag gctgagacca agcttcagca
     7261 tccctgactt tttcaaggaa atacgcatta ttctgctcca attgtgatgt ttgttgtttt
     7321 aaccctttgc agtgggttgt cgctgcctga ccttttttgt tttttgcccg gtcgggtcaa
     7381 cgttatgagg tggggatgcc gtactaatta acatcagtgt aacagtgcgg atcctccata
     7441 aaatgcccct cgtgacctac aatctgtcaa cagaatgtga aaacgtcaat acagccaacc
     7501 gggatttaca ccaacggtga gaatccacaa acaaagatta aaaaaacttc aaacagctat
     7561 ttgcagcaga gaaatttgtg ctacgccaac acaaccagaa caatcagctt aatatttagc
     7621 aacatttggt gattaagttt tatgcattta atgaaaaaaa tctgaggtaa aggtggatat
     7681 ctgagcgatt aataccatga acgctaaaac gcacagcgga aaatgccagc gccagccata
     7741 agtaacgcaa aaaaagattt aagcaaatat aaaaaaagac aatggtttct gtcacaaatc
     7801 cgttaatgga tagtgagata tggggcgcaa atttggcagg gaaatgtgtg gtgtgtagat
     7861 cggataaggc ggtcgcgccg catccgacaa tgggccggta catttgcctg atgcgacgct
     7921 ggcgcgtctt atcaggccta cgtcgagtcg aacctccccg cccccggggg ttctcttcct
     7981 catgagttac agatgcaaaa aagcctgctc gttgagcagg cttttcgaat ttggtcggtg
     8041 atagaggatt cgaacctccg accccttcgt cccgaacgaa gtgcgctacc aggctgcgcc
     8101 aatcaccgaa tgcggggcgc atcttactgc gcagatacgc cctcgtcaat cccttaatag
     8161 caaaatgcct tttgatcggc gagaaagtca gcaggcctct tagttagctg ccgcctctct
     8221 tgcctgcggg atgtggcacc agttgttgtt ttcgttaatt ccaccatccg gtgaagtata
     8281 gccaagacaa cccatgatgg tgtcgtacag ctctacatga cggcgtggca ctttcatgtc
     8341 ggcttctttt ttcagctgcg caaatgcctg cgcattgacc ggattttcca gatatttatc
     8401 cgacatccag accatcatcg gcacacggaa ctgttccggc ggtgccagtt cacgcggcgt
     8461 gccgtgcagg tgctcgcgtt cattaattga ctcaccgtgg tcagctgcat agaacacaat
     8521 cgctttctta tcgcgaacct gatcaatcac gctggaaata aagtgatcca cataggtcac
     8581 cgagttgtcg tatgagttga tcatctgcgc tttggtacag ccgctatcaa caccaataca
     8641 ttccggcttc cactgcgcaa agctgcgcgg gtaacgctgg gtgtagttaa agtgtgagcc
     8701 tttggtatgc agaatgatca gatgcttacc atccgggttg cgccccagcg attgctgcat
     8761 ttcgtctacc agcaacatat catctaccgg cttgccacgg ttacgtggct ccgcaccaat
     8821 ctgctcacga taagcaatgt tgtccgccat cgtattgctg tagaaccaca tttcgctttg
     8881 catagcgtag agatcagaac tgaatcctaa ctgcttcaat accgcgaaaa tgttctgttc
     8941 ttttaatgtg cgctgcggat tatcttctgc tccaccctga cgtacaaaca tgcaacgcag
     9001 tgagagtttg gttgcagtat cacaggagta accacggaac gccgccagat ttttctcctg
     9061 agccagcttc ggtgtggtat tgcgttcata gccgaaaatg cccatatgat cccagcgcgt
     9121 ggtttcacca ataatgaaaa cgacatatgt atcatcaacg ttttgtggag cctggtacgt
     9181 aaacttcttc gcagggttaa ggagagaatt gttatcggaa gattcatcga cccgcgccca
     9241 ggcataaaga cctaatgccg aaagccagtt tgatggcaga taagagttcg cgacgacgcc
     9301 gccataactc ggcaaatcaa cacctgtcgc cctctccact ttcttctgct ggatatccag
     9361 caaacgaatc ggtgcccaaa ccataatacc tgccagtacg acgaccgcca ggctgcgaat
     9421 acgttgccct ggggttcgca gttgtcggag caaggtgtaa cgacagcggt tattccagat
     9481 aaggataaga ggcaacgcgc ttacggcgat taaccagaga ataaagttca gaccaacaac
     9541 ttcttttgac aggtcgatat cggtggtcat gacggatgcg atgatgccat aaccaatgac
     9601 tacattaagg aaggtcatgt aatagctggc acctgcggaa aagagcacca ccagcgatgc
     9661 cagaatacgc cagctgcggc ggccaaacag cgaaagaaga cgcagtaaaa agaaggtcac
     9721 cagtacggtg gcggccagtt caacaacagc agaaatacct ttccagacgg taaaatcgtg
     9781 cgcatagctg ccgaagcggc ggtaaaaaac cgcgccattc ataaaaaggc caatatagat
     9841 tgcaagcaag aagctcagct tctgctgtgt aatcgatttg atgtatctca tgcaaacaaa
     9901 ccctggacaa acaggtgata acaccctgct tagtggcttt tcaggccagg caggggaaaa
     9961 gtgcggaggt agtcagataa gtaaaatcta cacgcgcgaa taagtagacc acagcaaagg
    10021 gaaaaagtgt ggggaaagag tgtgcacgaa gcacaaattt acaaaaatgc aagtttgaaa
    10081 agagacttta gcgttacaaa aagcagtaaa aagggccagt gagctagccc cggtgaagcg
    10141 aaattattcg tactgaaccg taaaatcgac cgtcgcatct gcgcggccaa tggttgcatc
    10201 agcacgcagc ggcacgtagc tggcgatata gttcagttga tgcgcgccat caccgttaat
    10261 cgcgaactca atcggcgtcg gtgtcgtatt tttcgggaac tgttgtactc ccgcagaggt
    10321 tttaattttc aaccccacgc ccgtagcacc acccgcatcc agcgcaaaaa gatttggatc
    10381 gtccgcattg ggtgttccat ccatggtgac gtaagccctg acaacttcgc tacagttttt
    10441 cagaccgatg gaaaaagtag tgggattaag ttctgttccg attcttactg gctttttgag
    10501 aaccgctaat cccatgtcaa cttcaacact gtcttttacc agttcacagg ctccctggct
    10561 gattttaaga gagaatttca acgttccttt cgcccctaag tctgtggttg ccgacagtga
    10621 cgaaaaggaa aaaggtagca ggcataatgc aagtattgtt cgtttcatca gtgctaatcc
    10681 aattcaacat gcattgttgc cacaccgcta taatcaccgg ctgcgggtaa tttcccggtt
    10741 gtactaaccg gataagcagt aatggtggtt gttactttac gtttgacata ttcgtacagt
    10801 gagccgggat tcatttctaa taagctatta ctatcattcg gcttaagaat attaccttta
    10861 ccatcctcaa ttaccgcacc aacatccgcg ttgcccaaac tgattgcgct ggggtaagcc
    10921 gcgtttggcg taccttcaag actcagatag atatggacac catcagaaat attagtacag
    10981 ttaaacgtta attctttttg tattttctgg acattttgtg gcggctgccc ggttcgacct
    11041 ttgaaatcat gggcctgata ctcaccaaag tcgatatcca atgatgtccc tgcggcaagt
    11101 tcacagcctt gagttacggt gacctttcct gaaagaagca cctgtgatat tggaactgaa
    11161 ctataaacat tctccttttt tgtaccaaac acagaaacta taatagtttg cggaatgttt
    11221 tgttccccaa caaaaggttt agcgatataa agtgagagct gtcctttact ccctgtagtc
    11281 cagccaaaag ttggttgatc gcactctctt cctggctggc tatttgattt attgataaat
    11341 ggaacctgta cattccccac gtttgcaata tatacccggg ttgaaacctc aagattattg
    11401 gtaattttaa aataggttga gttatgcccc gccgccaatg ttgactcagt tttatatagc
    11461 gttggtcgtg cttctgtcgg gttcggtgat ggacattcac aaacaccaga atatttatca
    11521 cccagtgccc attggtaaaa atcagggtag gtctgcccgg taacattatc cgtcggattt
    11581 tgaatatttt tggtgaatga tgtaacaaaa ataaaggggt caccacttgc cgtcctgcac
    11641 ggcccccaat ccgcagccca tatattcccg gtaaaggtgc tcgtccctaa aagcaccata
    11701 cctgccaaca agtttttaat tttcatctgc atatccttat ttatcgcata ccgcattaac
    11761 catcacgata cccgaattat cctttttctc tggcaagcgg aatggcacgc ggcaacgttg
    11821 cgcctgctcg tgcccccagc tcacctgcaa ttcgccctct tctggcatcc cgctgatata
    11881 aagctgtcct tcttcaccca caatgctgct ggcaggtttg ctctcatcac ttaacgctgc
    11941 ggtagcacca aacggtactg cgttgccatt cgcccgtttc aaactcatca gcacacgata
    12001 accgacacgc gtatcaaagc gggaacggac tatcgcccca cgcgttggaa cgactttctg
    12061 aaacgcttcc tgtaattcaa cgttttgccc taacgtgtcg ctgcgcaatt ccacaatggt
    12121 ttcacgatat ggcgtgagat acggcaccac tgcgttgccc atccagtcaa cttcaacgcc
    12181 gctgctattt actacatgtc caccatgcgc acccggcgca gaaacaattg ccagactttc
    12241 acccaagggt tgcgagagag tgataccttc actgtgaaca atgactccac cacgcacacc
    12301 atagttaact tgttggttat ctttggaata gttataaccc acgtcaatgt tgccagaacc
    12361 tcccatataa cccatgttga cactaccact gttgccaacc ccattactgg tataaccctg
    12421 ctgaacgtta tagttcaagt tacgatcttc cagtaatgtg ccgctgacag aggcctgttg
    12481 tgacgtcttg ccgttctggt cggtggtcat acgataactg ccccatgcgc cacccagcgg
    12541 tattgagagg ctaaatgacc acagtcgatc gtcttcatcc cattcagggc ttctcgtcca
    12601 ggtataggcg atactgtaat tcacacgacc aatatgaccg tgataaccag cgcttaagga
    12661 gttctcctta ccatcaacgt tccagtaatc ctgctgcgtc atattgaagt aaacagaacc
    12721 gtagtctccc agttgctgag tgatgttacc ttgtatctgg ctgcgcttat gataacgccg
    12781 atagtcactg tcggcgtcac tacggacatc ggtcgcttcc tggaaggtgt aataaccgct
    12841 ggttgagtac ttgtagccca acagacgcag ttcggtacca ccctcaaaac ttttcgaata
    12901 gaggaaacga taagactgcc cttcatcttt ttcattgttg cgtaactgac ttttcgcctg
    12961 ggtgacatcc accgataccg caccaatggt gccgaagttt ttccccagcc ccagagctaa
    13021 agcgttgtag tctgccgata gcaacgcacc accataggcg gtcattcccc acggcaagcc
    13081 gtacatggca gtaaattgcc cgaatcgcgg tttaccgctg tcgtaattac cggcacgata
    13141 ctcaccggca gcaaggctgt atttcagata cccttcacgc tggaaaatcg ccactgccga
    13201 aaatggttga ataaaacgtt gttcacttcc gtcagattct ttaatgatca ccgtcaagtc
    13261 accgctgccg gaggttgcgt agagatcgtt aatctcaaat gcgcccggct gaacaaaggt
    13321 acgataaata acatatccgt tctgttcgat actcacttca gcgttacttt tggcgatgcc
    13381 acgcaccacc ggagcaaagc cacgctggct atccggcaac atttcatcgt cggatgccaa
    13441 taatgccccg cgcatttgta cgctatcaaa aatctcgcca ggcgtggcgg tatcacccaa
    13501 tgttatctgt gacttgagtg gaataatcgc ccggtttaac gatgtaccaa cgttatccca
    13561 ttgatttttg ccatcggtac ggttccagat actgttatta cgtaaacgcc acggccccac
    13621 gttcaaaccg ctgcgcaggt tcaggtaata actgtcactg gtcgaatctg gagatgaacc
    13681 ggaaccttcg ttgcgaccat tgctgccact aaagctgtag tccagtaaca gcgcaggtat
    13741 tccttcgtcc cagcgagaag gatcaaccgt gccacgagcg gtcatcatca ttgctgcctg
    13801 tggaaaggta agatgcaacg tctgagtatt aaaatcaaaa cgactggtgg cctggggaat
    13861 gatttcatca aacgctacgc aggcttccgg tggcaatatc ttcagtgccg gaaagctgtc
    13921 gatgcgcacg cccatttctt ccagttgaac acggctaagg caaggcataa gcgactcgcc
    13981 gctggctttg cgttgtgctt cgctggcagt actaaaatcc agcgtccggg aagccatttt
    14041 ttcttcgttt acaaacacaa atacccgata tttaccgggt atttgctggc cgacaggaga
    14101 gtcgtaaact gacaaatcga cgtgttgatc aataccctga acattttcaa gaaatttagg
    14161 gttgaatttt ccgccgtacg caggtaacgc gctggcaata gttaatgcca acagtgagaa
    14221 agaaaaatgc gctctgtagt gatcaagagg taaatgcatc caatgcatag taaagcgcct
    14281 tgtaaaaaga tcagttaatc gacttcgtca gcatttcacc ggcgacaccg aaatcattaa
    14341 taatgctgta tttcacttcg ttcgcattgc ctgtaccggc tggaaggtta atgtttaacg
    14401 ttcctttagc gaggaccatt cccgcccgtt caatcttttg tccattaatg gacatctgat
    14461 taaaggtcag gttataagct gagtcgttat taaccacaat ttggttattt tgacgctgaa
    14521 atcgtaactg tttcattgct tcaaggttgc ttcccggcaa gcctttcgga cggtagaaca
    14581 acttcaaacg ggtgcgaacg gctatctgta agacgttttt attttcattt tcagcacttt
    14641 tcgcaggaat cgcttttaca ttaatccagt aaacggattc acgatctttg ggtaaggtat
    14701 cttcaatatt aacaatacgt aagacgttat ttttatcagg atcaagtttg aataatggcg
    14761 gtgtaataat aaacggcgtt tttttatttc cattcacatc atcaatccat gactggatca
    14821 aaaactcttc atttttatta tggttttcaa cggtcagcga cgcttctttc ttgccaccat
    14881 cataaataac gcgcgtcgtg cttagggcta cgccgccagc aaaacactgt tgtgcggtca
    14941 ttaaaagcgc cagaacggca aggctaattc gtttcatgat tcggtccctg agtcattggt
    15001 gtgaataatg gcaacagata atctgttgcc attgagagat aatcgttccg tctggatcga
    15061 ttagttgtaa gtcaggttga agtcgacttc ggcattaccg taaccgatgg ttactgcatt
    15121 tttggtagcg acatagttag cggtaaaata cagaacagtc tggccttctt tcaggacggt
    15181 agcagattta ccggtattca gatcaaccag cgtattggta ttatcataaa taccaatacc
    15241 cacgccagtt gcggcacctg gttcagtgct tacagaaatc agtttgctgt tgtctgcgtc
    15301 gccaacaccg ttaaagctga tctccacatt tttgaaagtg gagatatcgc agttttccag
    15361 tttgattttg aacggagttg ctgcggattt atcaccagta gtggtaaaca cagacttgtt
    15421 cacctggccc agaacaactt cttgtttttg agtgtctgga gacacaacac aagcggcgtc
    15481 aacaatctct ccggtgaatt taacagaacc gtcgcctgca tcagctgcga atacggaagt
    15541 agaggtcagc gcaagcgcag ataaggcaaa cagaaccttt ttcatttata actccattt
//
LOCUS       DADSZD010000103        15412 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.104, whole genome shotgun sequence.
ACCESSION   DADSZD010000103 DADSZD010000000
VERSION     DADSZD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.104"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..295)
                     /locus_tag="J1Z28_002971"
     CDS             complement(<1..295)
                     /locus_tag="J1Z28_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBA7717786.1"
                     /translation="MAEHDITPEVALSKTQRRLKVGYVGISHTNRKTKVPTRYSRSPS
                     LHLKGNWLKEAGFDTGRGVTVKISEGCLTIIADSDEVQKLREELYQVKQSVKGM"
     gene            430..618
                     /locus_tag="J1Z28_002972"
                     /pseudo
     CDS             430..618
                     /locus_tag="J1Z28_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            769..1278
                     /locus_tag="J1Z28_002973"
     CDS             769..1278
                     /locus_tag="J1Z28_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBA7717787.1"
                     /translation="MNQQRFDDSTLIRIFALYELHRLKSHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            1275..2693
                     /gene="phrB"
                     /locus_tag="J1Z28_002974"
     CDS             1275..2693
                     /gene="phrB"
                     /locus_tag="J1Z28_002974"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBA7717788.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFAASVETVKQVCAENRITHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(2735..4216)
                     /gene="dtpD"
                     /locus_tag="J1Z28_002975"
     CDS             complement(2735..4216)
                     /gene="dtpD"
                     /locus_tag="J1Z28_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBA7717789.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFAHTRGVNKTVLCARNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDIFGYTVPTAMFQSINAFAVMLCGVFLAWMVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            4487..5230
                     /gene="ybgI"
                     /locus_tag="J1Z28_002976"
     CDS             4487..5230
                     /gene="ybgI"
                     /locus_tag="J1Z28_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBA7717790.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5253..5909
                     /gene="pxpB"
                     /locus_tag="J1Z28_002977"
     CDS             5253..5909
                     /gene="pxpB"
                     /locus_tag="J1Z28_002977"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBA7717791.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLVGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            5903..6835
                     /gene="pxpC"
                     /locus_tag="J1Z28_002978"
     CDS             5903..6835
                     /gene="pxpC"
                     /locus_tag="J1Z28_002978"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBA7717792.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            6825..7559
                     /gene="pxpA"
                     /locus_tag="J1Z28_002979"
     CDS             6825..7559
                     /gene="pxpA"
                     /locus_tag="J1Z28_002979"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBA7717793.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQYGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAAKGIVVAA"
     gene            7595..8386
                     /gene="nei"
                     /locus_tag="J1Z28_002980"
     CDS             7595..8386
                     /gene="nei"
                     /locus_tag="J1Z28_002980"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBA7717794.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(8383..9429)
                     /locus_tag="J1Z28_002981"
     CDS             complement(8383..9429)
                     /locus_tag="J1Z28_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBA7717795.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(9581..9958)
                     /locus_tag="J1Z28_002982"
     CDS             complement(9581..9958)
                     /locus_tag="J1Z28_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7717796.1"
                     /translation="MIDLGKFNLLDIIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSF
                     YTEKTLVEEDKALLIGNGLKLRILDENASPYTFNKYAEYADFTSDMLIYEKTYTAELS
                     STPGTPIAAGPFDTVVLFKINYN"
     gene            complement(10106..11389)
                     /gene="gltA"
                     /locus_tag="J1Z28_002983"
     CDS             complement(10106..11389)
                     /gene="gltA"
                     /locus_tag="J1Z28_002983"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBA7717797.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(11595..11702)
                     /locus_tag="J1Z28_002984"
     CDS             complement(11595..11702)
                     /locus_tag="J1Z28_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717798.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            12083..12487
                     /gene="sdhC"
                     /locus_tag="J1Z28_002985"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             12083..12487
                     /gene="sdhC"
                     /locus_tag="J1Z28_002985"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA7717799.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            12481..12828
                     /gene="sdhD"
                     /locus_tag="J1Z28_002986"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             12481..12828
                     /gene="sdhD"
                     /locus_tag="J1Z28_002986"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBA7717800.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            12828..14594
                     /gene="sdhA"
                     /locus_tag="J1Z28_002987"
     CDS             12828..14594
                     /gene="sdhA"
                     /locus_tag="J1Z28_002987"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBA7717801.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRRALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            14610..15326
                     /locus_tag="J1Z28_002988"
     CDS             14610..15326
                     /locus_tag="J1Z28_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HBA7717802.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
BASE COUNT         3754 a         3800 c         4204 g         3654 t
ORIGIN      
        1 gcatcccctt aactgactgt ttaacctgat aaagttcctc ccgcagtttc tgcacctcgt
       61 cactgtcagc aatgatggtc aggcatccct ccgaaatctt caccgtgaca ccccgtccgg
      121 tatcaaatcc ggcctctttc agccagttgc ccttaaggtg caggcttggg ctgcgcgaat
      181 atctggtggg gactttggtc ttacggttgg tgtgggaaat gccgacatag ccgactttaa
      241 ggcgacgctg tgttttggaa agggcgactt ctggcgtaat atcgtgttca gccatgatta
      301 actacctctg ttagttgctt gtggtgagcg gtggttaagg gttgccgccc ttaaccactg
      361 cgttatttat cagtcagtta cgcgcctgca caatcagggc gcttaagatc tctgaaatca
      421 tcttctgctt atttattcgc tgtaaaagga aaccaggggc ggctaaataa agccttcgag
      481 gaaaaatttc cgctgaaaga atcaaataat ccagagcatg acagttacgc aatgagtgaa
      541 aagagtcacg gcagagaaga aatccgtctt catattgttt gtgatgtccc tgatgagctt
      601 attgatttca cgtttgaata gaaagggcag ctatggatag aaacaacctg acgtcagtcc
      661 ttgcggggag cgggctttcg taaatttgtc ctgctacaac aggattaact tcacaaatat
      721 catttctcaa cgtctacact tactcctgtt aatatctcag gagcagtaat gaatcagcaa
      781 cgatttgatg acagcactct aatccgtatc ttcgcgcttt atgagttaca tcgactgaaa
      841 tcacacggct taacgcgcgg ggcgcttctc gattatcaca gccgctataa actcgtcttt
      901 ctggcgcatt ctcagccgga gtaccgcaaa cttggcccgt ttgtggctga tattcaccgg
      961 tggcaaagtc tggatgacta ttacaaccag taccgccaac gcgtgattgt tttgctttct
     1021 caccctgcca accggcgtga tcatactaac gttttgatgc acgttcaggg atattttcgc
     1081 ccacatattg attccacaga acgccagcag ctggcggcgc ttatcgacag ttatcgccgt
     1141 ggcgagcaac cgcttcttgc gccgctgatg cgtatcaaac actatatggc gctttatcct
     1201 gacgcctggc tttcagggca gcgttatttc gaactttggc cgcgtgtgat taacttgcgc
     1261 catgcaggag ttttatgact acccatctgg tctggtttcg ccaggattta cgtcagcacg
     1321 ataatctcgc gctggctgcc gcctgccgca attcgtctgc gcgcgtgctg gcgttgtata
     1381 tcgctacacc acgccagtgg gcggcgcata atgtgtcgcc gcgtcaggct gaacttatca
     1441 atacacaact taatgcgctg caaaacgcgc tggcggaaaa aggcattcct ttattgttcc
     1501 gcgaagtgga tgactttgct gccagtgtcg aaacagttaa acaggtgtgc gcggaaaatc
     1561 gcattaccca tctgttttat aactatcagt atgaggtgaa tgagcgggcg cgggatgtgc
     1621 aggctgagag gacgctgcgt aacgtggtgt gtgaaggatt tgatgacagc gtgatcctgc
     1681 cgccgggggc ggtgatgact ggcaatcatg agatgtacaa agtctttacg ccttttaaga
     1741 atgcctggct gaaacggctg cgggaaggga tgccggagtg cgtcgctgcg ccaaaagtac
     1801 gtagtagtgg atcgatagag cccgcgccat ccattacgct gaattatcct cgtcagtctt
     1861 tcgatactgc gcatttcccg gtggaagaaa aagcggcgat tgcgcaatta cgccagtttt
     1921 gccagaacgg tgccggagag tatgagcaac aacgagattt tccggcagtg gaaggcacca
     1981 gtcgtttgtc cgccagcctg gcaacgggcg ggttatcgcc tcgccagtgt ttacatcgcc
     2041 tgttggcgga acaaccgcag gtgctggaag gcggaccggg gagcgtctgg cttaatgagc
     2101 tgatatggcg cgagttctac cgccatttga tgacgtatta tccttcgctg tgtaaacatc
     2161 gtccgtttat tgcctggacg gatcgtgtac agtggcaaag caatcccgcc catttaaagg
     2221 cctggcagga gggcaaaacg ggatacccga ttgtcgatgc tgccatgcgt cagcttaaca
     2281 gcactggctg gatgcataac cggctacgga tgattacagc cagttttctg gtgaaagatt
     2341 tgttgatcga ctggcgcgaa ggcgagcgat atttcatgtc gcagttgatt gatggtgatt
     2401 tggcagctaa taacggtggc tggcagtggg ctgcttctac gggtactgat gcagcgccgt
     2461 attttcgtat tttcaacccg acaactcagg gcgagaaatt tgaccgcgaa ggcgaattta
     2521 tccgccagtg gctaccggag ctgcgcgatg taccggggaa agcggtgcat gagccgtgga
     2581 agtgggcaga gaaaacaggt gtgacgttgg attatccgca accgatagtc gatcacaaag
     2641 aggcaaggct gcgaacgctg gcagcgtacg aagaagcgag gaaaggagcc tgataaaacg
     2701 cagcgagcgt tttatcaggc atggtgctgg cttattaaga ctccagcgcc agcgcgcggt
     2761 ttctgaactt aagcgcctga tacagccaaa tcatcagtac cagtcctaca cacgccagtg
     2821 cgccccaggt aatttgatca aatacttcaa tatatgcatt gatggagtag ttgatcgccc
     2881 cggaagcatc aaacgaagcc tgcgatgtct gatcggcaat cacgcccgcc agatagttcg
     2941 caatcgcgcc ggaaagcagc atataaatgc cggttaatac gccggtcacg ccggggattt
     3001 caatgcgcgt aatctgcgac atagcaaccg gatcgataaa cagttccgca aagcccatca
     3061 ccgccaggcc taacaccatc agtggcagag aagagtgacc atacattgcg gaccagcggg
     3121 cgcttaaggt cagaatgcag aatccggcgc tcatcaggcc aaggccaaga gcaaattttc
     3181 cccagatgcg tacggtacga ttacctgcga cgctttcttt aaccatccac gccaggaaca
     3241 caccgcacag cataactgcg aaggcattaa tcgactggaa catagcagtc ggaacggtat
     3301 aaccaaatat atcgcggtta acgaagcggt cgatataaag gctaatcgag ctacccccct
     3361 gttgtgcgaa ggcccagaac aacatactga agaaggtgag cgtcacaatc agtcccagct
     3421 ctttacgctg tttctggttt tctgctttgc gataaatttt tgccagaacg ccaaggccaa
     3481 tgatagtcgc gacaatcaag gcgtataccg accactcttt ccagaacagt acggtaatca
     3541 gcgcaggcgt tgccaccagc agaaccagca gccatcccca gttcggcagc agaaagtttc
     3601 tggcgcacag caccgttttg ttaacgccac gggtatgagc aaaatgacga ttgccacata
     3661 agaagataac cagacccgcg atcataccaa ccgccgccag accaaagccc atcgcccaac
     3721 tgtactcttc ctgggcataa ccacaggcga tgggtgcgat aatagacccc acgttacccg
     3781 ccgcatacat cagcgagaaa ccgccatcac gacgtggatc ggttggctca tacagctcgc
     3841 caagcagaca gcttacgtta gatttaaaca ggccatagcc gcagacgata atcgccaggg
     3901 acagatagag gaatgacgga tggatctcac tggcacccag caccacatga ccgatcgcca
     3961 tcaacaacgc ccccagcatc accgccatgc gattgccgag aactttatcc gccaaaaagc
     4021 caccgaggat tggcgtgaca tataccagcg aacagtaggc gctaaataac tcgtaggcgt
     4081 gattatcgct atattttagt tgattggtga gatagaggat cagcagggca cgcatgccgt
     4141 aaaagctaaa atattcccag atttgcagcg caacgacata gtaaatagcg cgcggttgtg
     4201 atgcgtgttt attcataata attccacaat tgactgcaac gtcacgtaat agttgcgcgg
     4261 cgtgcttgtg tttccttaaa gttaaaactt tgatacagat ctgattattt atgcaatatg
     4321 ctgtctgatt gcataaatat acattagctg aagcgtgatg atataaacaa tttgcgtctt
     4381 ttggcgtcgg gtttgtttct taatatgtca aaagattggc tatcgacgaa caggcgaggt
     4441 aacgctatgt taacggtgta gctatcagaa tacggaaggc gcgaagatga aaaacaccga
     4501 actggaacaa ctgattaacg aaaaactgaa cagcgcggcg attagtgatt acgcgccgaa
     4561 tggtttgcag gtggaaggca aagagacggt gcaaaaaatt gttaccggtg tcactgccag
     4621 ccaggctttg ctcgatgagg cagtgcgtct gggcgcggat gcggtcattg tgcatcatgg
     4681 ctacttctgg aaaggcgaat ctccggtcat tcgcggaatg aagcgtaacc gcttaaaaac
     4741 attgctggcg aatgatatca acctgtatgg ctggcatttg ccgcttgacg cacatcctga
     4801 gctgggcaat aacgcacagc tggcggcgtt actggggatc acggtcatgg gcgaaattga
     4861 gccgttggtg ccgtggggcg aactgaccat gcctgtgccg ggactggaac tggcctcctg
     4921 gattgaagcg cgtctgggac gtaagccgtt atggtgtggc gataccgggc ctgaggtggt
     4981 ccagcgcgtc gcctggtgca cggggggcgg gcaaagtttt atcgatagcg ccgcgcgttt
     5041 tggcgtggat gcttttatta ctggcgaagt ttctgaacag accattcatt cagcccgcga
     5101 gcagggattg catttttatg ctgcgggtca ccatgccact gaacgcggtg gtattcgcgc
     5161 attgagcgag tggctgaatg aaaataccga tcttgatgtg acctttattg atattcctaa
     5221 tcctgcataa cgaataatca gagggatcga aagtgcaacg agcgcgttgt tatctgatag
     5281 gtgaaacggc ggtagtactg gaactggaac cgccggtgac gctggctagc cagaaacgga
     5341 tctggcgact ggcacagcgt ctggtggata tgccgaatgt ggttgaagcc attcctggca
     5401 tgaacaatat cacagtgatt ttgcgtaatc ctgagtcgct ggcgctggat gccatagagc
     5461 gtttgcaacg ctggtgggag gagagtgagg cgctggagcc ggagtctcgc tttattgaaa
     5521 ttccggtggt ttacggtggc gcagccggac cggatttggc ggtggtcgcg gcgcattgtg
     5581 gattgagcga aaaacaggtt gttgaattgc actcctccgt ggaatacgtg gtctggtttt
     5641 taggttttca accgggcttc ccgtatctcg ggagtttgcc ggaacaacta cacacgccac
     5701 ggcgcgcaga accgcgcttg cacgttccgg caggttctgt tgggatcggc gggccgcaga
     5761 ccggtgttta tccgctggca acgccgggtg gctggcagtt ggttggtcat acctcactca
     5821 gcctgtttga tccggagcgt gacgaaccca tcttattacg tccgggagac agcgtgcgct
     5881 ttgtacccca gaaggaggga gtatgctgaa gattattcgt gcgggcatgt acaccaccgt
     5941 gcaggatggc ggccgtcacg gctttcgtca gtctggtatc agccactgtg gcgcactgga
     6001 tatgcctgcg ctgcgcattg ctaacctgct ggtgggtaat gacgccaacg cccccgcgct
     6061 ggagatcacg ctcggtcagt taacggttga gttcgaaact gatgggtggt ttgccctgac
     6121 gggtgccggt tgcgaagcgc ggctggatga taacgccgtc tggaccggct ggcgattgcc
     6181 aatgagagca ggccagcgtt taacgcttaa acgcccgcag cacgggatgc gcagttatct
     6241 ggcggtcgcg ggtggtattg atgttccgcc ggtaatggga tcatgcagca ccgatctcaa
     6301 agtggggatt ggcgggttgg aaggccgttt actgagggat ggtgaccgac tcccgattgg
     6361 caaagcgaag cgtgatttta tggaagcgca gggcgttaaa cagctgctgt ggggcaaccg
     6421 cattcgcgcc ttgccggggc cggaatatca tgagttcgat cgcgcctcgc aggatgcatt
     6481 ctggcgttcg ccctggcagc ttagctcgca aagtaaccgg atgggctatc gcttacaggg
     6541 gcaaatttta aaacgcacca ccgatcgcga actgttatct cacggtttgt taccaggtgt
     6601 ggtgcaggtg ccgcataacg ggcagcccat tgtgttgatg aacgatgcgc aaaccaccgg
     6661 tggttacccg cgtattgcct gtatcattga ggctgatatg taccatctgg cgcaaattcc
     6721 gctcggtcag ccgattcatt ttgtccagtg ttcactggaa gaggcactga aagcacggca
     6781 agatcaacaa cgttatttcg aacaattagc gtggcggctg cacaatgaaa attgacctga
     6841 acgccgatct gggcgaaggc tgcgccagcg acgcagagct attaacgctg gtttcctctg
     6901 ccaatattgc ctgtggattt catgcaggcg atgcgcaaac catgcaggct tgcgtgcgtg
     6961 aagcaataaa aaacggtgtc gcgattggcg cgcacccgag ttttcccgac agggaaaatt
     7021 ttggtcgcag cgccatgcag ctgccgccag aaaccgtttt cgcccagacg ctgtatcaaa
     7081 ttggcgcgct ggcagcgatt acccgtgcgc aaggcggcgt aatgtgtcat gtcaaaccgc
     7141 acggcatgtt atacaaccag gcggcgaaag aggcacaact ggcagacgcc atcgccagag
     7201 cggtatacgc ttgcgatccg gcattgattc tcgtcgggct ggcaggaagc gagctgattc
     7261 gtgcaggcga acgatatggc ctggtaacgc gcgaggaagt gtttgccgat cgcggttatc
     7321 aggcagacgg ttcgctggtg ccgcgaagcc agccaggcgc gttgattgaa aacgaagaac
     7381 aggcgctggc gcaaacgttg gagatggtgc aatacggcag agtcaaaagt atcaccggcg
     7441 aatgggcaat ggtcacggcg caaacggtct gcctacatgg cgatggcgag cacgcactgg
     7501 cgttcgcccg ccgattacga gctacatttg ctgcaaaggg gattgttgtc gcagcataac
     7561 cccgcttaat aaagaatgaa aaaaggatat caccatgcct gaaggcccgg agatccgccg
     7621 tgcagcggat aacctggagg cggcgatcaa aggcaaacca ctaactgatg tctggtttgc
     7681 cttcccgcag ttaaaatctt atcaatcacg acttatcggt caacacgtta cccatgtgga
     7741 aacgcgtggt aaggcgttgt taactcattt ttccaacgac ttaacgctct acagccataa
     7801 tcagctttac ggcgtctggc gcgtggttga taccggcgaa gagccgcaga ccacgcgagt
     7861 attgcgggta aaactgcaaa cggctgacaa aaccattctg ctttatagcg cctcggatat
     7921 tgagatgttg actccggaac aactgaccac gcatccgttt ttacaacgcg ttggtcccga
     7981 tgtgctggat ccgaatctga cgccagaggt ggtaaaggaa cgattattgt cgccgcgctt
     8041 tcgtaaccgt cagtttgctg gattactgct cgatcaggcg tttctggcag gacttggcaa
     8101 ttatttgcgg gtggagatcc tctggcaggt tgggttgacc ggaaatcata aagcgaaaga
     8161 tctcaatgcg gcgcaactgg atgcactcgc acacgcgtta ctggatattc cgcgactttc
     8221 ttacgctacg cgggggcagg tggatgaaaa caaatatcat ggggcgctgt ttcgctttaa
     8281 ggtttttcat cgtgatggcg aaccttgcga acgctgtggc ggcatcattg agaaaaccac
     8341 gctgtcatcg cgcccgtttt actggtgccc aggctgccag cactaggccg aacgcttcgg
     8401 agcataggtt gaaataaacc gtgcgatggc aggccccgtc agcaaaatac tgaacaggcg
     8461 cagggtttgc atcgccatga tgagcgccat atcggcattg ctccctgcgg cgatgaccgc
     8521 cacggtatca agcccgccgg ggctggtggc gaggtaggcg gtcataaaat caatatgcat
     8581 aaaccgggtc agcccccacg ccatacccgc acaaatagcc agcagagcaa aaatcgacag
     8641 caggatttgt ggcagtgggc gcaatgcccg cagtaatatt tgcttatcga aaccaagacc
     8701 aatccgccag ccaattgcca tatatgccat cgccagcagc cattccggga gttcaatcgt
     8761 gatgagctga cctgactgga gcactgcgcc cgccagcatc gggatgagca tcgtgccgga
     8821 aggaagacgt agcaagcatc ccgccgtacc ggcaacgacc gcgagcaaaa tcgtaagcag
     8881 gagattaatg ctcactggcg ggaaccagac gatctgctgg ttaaccgctt cagcgttatc
     8941 gcccagcatc atgcgggtga ccagaacagc ggctcctgcg acgaacagca ctcgcagata
     9001 ttgcataaac gccaccagac gaatatctgc gccgtaatct tgcgccattg cgaccattgc
     9061 cgccgcgccg ccaggagaag aaccccaggc cccggtattt cctggcagcg agctatagcg
     9121 caccaataac caacccacga tggcgctgga aagcagcgtc accagtaaaa tcgcaagcac
     9181 gatcggccag ttgacggcca gcgtggtgag aatcgaacca gtgagatttt gtgcaatcat
     9241 gcagccaaga atggcttggg cagcgagaaa agcggagcgg ggaagttgca gagtgactcc
     9301 gcgcatactg aagatgatcc cggcgatcat cgggccaagc aggagtgccg ccgggagatg
     9361 taaagcgagg aagccaatag aaaggagaag tgacagaaca cataacattc cccactgcaa
     9421 tacaggcatt cttcgcttcc tcattatttt atctttggtg tatcgataca gcataaagaa
     9481 agctgggtct ggaaaaaata acggtaatca aggaaaaggg gaaaatcagc aataatctga
     9541 atacgttgcc ctttccggct gaaaagggcg tcgtgttaaa tcagttatag ttaatcttaa
     9601 aaagtaccac tgtatcgaag ggacccgccg cgatgggagt gccaggtgtg gacgataatt
     9661 cagccgtata ggttttttcg tagatcagca tatcactggt gaaatcggcg tattcagcat
     9721 atttattgaa tgtataaggc gaggcgtttt catccagtat acgtagcttc agaccattac
     9781 caatcaacaa tgctttatct tcttcaacca acgttttttc ggtataaaac gaggagctga
     9841 ccttaaatcc gtcggtacac tggtcatttt gcgattttgt tgtcttgata ctgaatgttt
     9901 tcgacattgt gtggcggcga ataatatcca gtaagttaaa tttacccaaa tcaatcacca
     9961 gtgtttccgg tgaaatattt tttcaccatt attaatgggt atttgcatct ggtttaattt
    10021 ttacaatttt ttatgttcag ccatataaaa agaacccgct ctattaaagg cgggtccgga
    10081 aagtgaacgg cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
    10141 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
    10201 tccagtgggc gatccagcca accgtacgtg ccatagcgaa gatcacggtg aacatagaag
    10261 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
    10321 ttttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
    10381 gcaggtcatc tttggtaccc agctctttca gaacttcatg gcaggtttca cgcattacgg
    10441 tggcgcgtgg gtcgtaattt ttgtacacac ggtgaccgaa gcccatcagg cggaaagaat
    10501 catttttgtc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctgatttctt
    10561 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
    10621 tacctgcagc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
    10681 aggtggaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
    10741 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
    10801 taccggcgta ggagagatcg ttgcgcgggt aaacaaatgg ctgaccaatg gaatacttgt
    10861 aacacatcgc ggccatggtc ggcatttttg acagcaggcg gaacgcggca atttcacgat
    10921 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc tgccagcgcg ccggtaatac
    10981 cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga cgggtaatct
    11041 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
    11101 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
    11161 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
    11221 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
    11281 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
    11341 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
    11401 ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
    11461 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
    11521 ctctgggcgc ttgcaggtaa aggatctatt gatgacgaat aaatggcgaa tcaagtactt
    11581 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
    11641 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
    11701 attatatgct tttcctggta atgtttgtaa caactttgtt gaatgattgt caaattagat
    11761 gattaaaaat taaataaatg ttgttatcgt gacgcagatc actgttcggg ataaaacccg
    11821 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
    11881 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
    11941 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
    12001 cggaggaagg aaatcccgac gtctcctggt aacagaaagt taacctctgt gcccgtagtc
    12061 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
    12121 aagacctgtt aatctggacc tacagaccat ccggttcccc gtcacggcga tagcgtccat
    12181 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcttgc tgtggcttct
    12241 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
    12301 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcatatcacg tcgtcgtagg
    12361 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
    12421 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
    12481 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgt
    12541 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgctaccagt
    12601 ggcgagctga catatgaagt ctggattggt ttcttcgcct ctgcgttcac caaagtgttc
    12661 accctgctgg cgctgttttc tatcttgatc catgcctgga tcggcatgtg gcaggtgttg
    12721 accgactacg ttaaaccgct ggccttgcgc ctgatgctgc aactggtgat tgtcgttgca
    12781 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
    12841 tcagagaatt tgatgcagtt gtgattggtg ctggcggcgc aggtatgcgc gcggcgctgc
    12901 aaatttccca gagtggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
    12961 ataccgtttc tgcgcaaggt ggtattaccg ttgcgctggg taatacccat gaagataact
    13021 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
    13081 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actggaacat atgggcctgc
    13141 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
    13201 acttcggcgg cgagcaggcg gcacgtactg cggcggctgc cgaccgtacc ggtcacgcac
    13261 tgttgcacac cctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
    13321 atgcgctgga tctggtgaaa aaccaggatg gcgcagtggt cggttgtacc gcactgtgca
    13381 tcgaaactgg tgaagtggtt tactttaaag ctcgcgcgac agtgctggcg actggcgggg
    13441 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
    13501 tggctatccg tgcaggcgta ccggtacagg atatggaaat gtggcagttc cacccgaccg
    13561 gtattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
    13621 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
    13681 gccgtgacgt ggtggcgcgt tccatcatga tcgaaatccg tgaaggccgc ggctgtgatg
    13741 gtccgtgggg gccacacgca aaactgaaac ttgaccatct ggggaaagaa gttctcgaat
    13801 cccgtctgcc gggtattctg gaactctccc gcaccttcgc tcacgttgat ccggtgaaag
    13861 agccgattcc ggtaattcca acctgtcact acatgatggg cggtattccg accaaagtga
    13921 ccggtcaggc gctgactgtg aatgagaaag gcgaagatgt ggttgttccg gggctgtttg
    13981 ccgttggtga aatcgcttgt gtatcggtac atggcgctaa ccgtctgggc ggcaactcgc
    14041 tgctggacct ggtcgtattt ggtcgtgcgg caggtctgca tctgcaagag tctatcgccg
    14101 agcagggcgc actgcgcgat gccagcgagt ctgatgtaga agcgtctctg gatcgcctga
    14161 accgctggaa caataaccgt aacggtgaag atccggtggc gatccgtaga gcactgcaag
    14221 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
    14281 agcagttgaa agttatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
    14341 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
    14401 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcgcatagc cgcttcgact
    14461 tcccggatcg cgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat
    14521 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
    14581 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
    14641 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
    14701 gtgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
    14761 tccgtcgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggtaaga
    14821 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
    14881 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
    14941 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
    15001 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggttgtat gaatgtattc
    15061 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
    15121 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
    15181 acagccgcct cgacggtttg agcgatgcat tcagtgtatt ccgctgtcac agcatcatga
    15241 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catatcaagt
    15301 cgatgttgtt gcaacgtaat gcgtaaaccc aggcctgata agacgcacaa gcgtcgcatc
    15361 aggcaaccag tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag aa
//
LOCUS       DADSZD010000104        15208 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.105, whole genome shotgun sequence.
ACCESSION   DADSZD010000104 DADSZD010000000
VERSION     DADSZD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.105"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            142..870
                     /gene="gadW"
                     /locus_tag="J1Z28_002989"
     CDS             142..870
                     /gene="gadW"
                     /locus_tag="J1Z28_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBA7717803.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(1233..4346)
                     /locus_tag="J1Z28_002990"
     CDS             complement(1233..4346)
                     /locus_tag="J1Z28_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBA7717804.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4371..5528)
                     /gene="mdtE"
                     /locus_tag="J1Z28_002991"
     CDS             complement(4371..5528)
                     /gene="mdtE"
                     /locus_tag="J1Z28_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBA7717805.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5588..5917
                     /locus_tag="J1Z28_002992"
     CDS             5588..5917
                     /locus_tag="J1Z28_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717806.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(5867..6394)
                     /gene="gadE"
                     /locus_tag="J1Z28_002993"
     CDS             complement(5867..6394)
                     /gene="gadE"
                     /locus_tag="J1Z28_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBA7717807.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7193..7765)
                     /gene="hdeD"
                     /locus_tag="J1Z28_002994"
     CDS             complement(7193..7765)
                     /gene="hdeD"
                     /locus_tag="J1Z28_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBA7717808.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            8020..8352
                     /gene="hdeA"
                     /locus_tag="J1Z28_002995"
     CDS             8020..8352
                     /gene="hdeA"
                     /locus_tag="J1Z28_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBA7717809.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            8468..8794
                     /gene="hdeB"
                     /locus_tag="J1Z28_002996"
     CDS             8468..8794
                     /gene="hdeB"
                     /locus_tag="J1Z28_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBA7717810.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            8858..9505
                     /locus_tag="J1Z28_002997"
     CDS             8858..9505
                     /locus_tag="J1Z28_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBA7717811.1"
                     /translation="MTAEFIIRLILAVIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(9557..10327)
                     /locus_tag="J1Z28_002998"
     CDS             complement(9557..10327)
                     /locus_tag="J1Z28_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HBA7717812.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVTA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(10324..11280)
                     /locus_tag="J1Z28_002999"
     CDS             complement(10324..11280)
                     /locus_tag="J1Z28_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7717813.1"
                     /translation="MGLSLLLLALVLFGASQGALKISFDALFDEEYRDIWLNIRLPRV
                     LLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIVLPFSFPVVLVLY
                     EQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAAIGILSYIGDEQQ
                     LRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQLGDEEAHYLGVNV
                     KRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRWLIPCSALAGACL
                     LLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(11365..11988)
                     /gene="chuY"
                     /locus_tag="J1Z28_003000"
     CDS             complement(11365..11988)
                     /gene="chuY"
                     /locus_tag="J1Z28_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HBA7717814.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAAPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(11988..12482)
                     /gene="hutX"
                     /locus_tag="J1Z28_003001"
     CDS             complement(11988..12482)
                     /gene="hutX"
                     /locus_tag="J1Z28_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HBA7717815.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAASL
                     KEHA"
     gene            complement(12495..13832)
                     /locus_tag="J1Z28_003002"
     CDS             complement(12495..13832)
                     /locus_tag="J1Z28_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HBA7717816.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNEDACAHYTDALIREIEREAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAVVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
     gene            complement(13852..14766)
                     /locus_tag="J1Z28_003003"
     CDS             complement(13852..14766)
                     /locus_tag="J1Z28_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter substrate-binding protein"
                     /protein_id="HBA7717817.1"
                     /translation="MPRIITRPFLFSPLTLCISAVVSAAKTMVKRKKLFTALLALSWT
                     FSVTAAERIVVAGGSLTELIYAMGAGKRVVGVDETTSYPPETAKLPHIGYWKQLSSEG
                     ILSLRPDSVITWQDAGPQIVLDQLRAQKVNVVTLPRVPATLEQMYANIRQLAKTLQVP
                     EQGEALVTQINQRLERVQQNVAAKKAPVKAMFILSAGGSAPQVAGKGSVADAILSLAG
                     AENVATHQQYKSYSAESLIAANPEVIVVTSQMVDGDINRLRSIAGITHTAAWKNQRII
                     TVDQNLILGMGPRIADVVESLHQQLWPQ"
BASE COUNT         3978 a         3632 c         3670 g         3928 t
ORIGIN      
        1 taactgttat gcaaaactac tttgtggata aaatttggtc ctaccaaata tggcagtttt
       61 tgcacacaga aacagtctgg cgtcatttca ttagtatact gacattgaaa taatcgcagt
      121 aatgaaatat aagggatagt catggctcat gtctgctcgg tgatcctcgt tcgtcgttca
      181 ttcgatattc atcatgaaca gcaaaaaata tcgttgcata acgagagtat cctgctgctg
      241 gataaaaatt tggcagacga ttttgcgttt tgttcactgg atacgcgacg gctggatatc
      301 gaagagctga cagtttgcca ttacttacaa aatattcgtc agttgccacg caatttagga
      361 ttgcatagca aagaccgttt gttaattaac cagtcacccc ccatacagct ggtgacggcg
      421 atttttgata gtttcaatga cccccgggtc aattcgccga tactgagcaa aatgctctat
      481 ctttcctgtt tatcaatgtt ttctcataag aaagaactga tccccttact tttcaatagt
      541 atcagtactg tttcaggaaa agttgaacgc cttattagct ttgatatcgc taaacgttgg
      601 tatctacgcg atatcgcaga aagaatgtat accagcgaga gtctcatcaa aaaaaagttg
      661 caggatgaaa atacctgttt cagtaaaata ttactcgcct ccaggatgtc gatggccaga
      721 cgattactcg agttacgtca aatacctctg catactattg cggaaaaatg tggctatagc
      781 agtacgtcat actttataaa tacatttaga caatattatg gtgtaacgcc acatcagttt
      841 tcgcaacatt cgccgggtac cttttcctga catatttcgc atttgaatat tggtcaggat
      901 ctcacgtctg cttcatgtga aactctttcc tgatgatttc tgccgggcta ccggctagtt
      961 ctctttcgca gtttgccagg cggtataggt attttcagga ggaaaagact cggcatgttt
     1021 gggattatta agctaacaat tcatacgatt acgggaatgt gggtcagtat tgtgctgttc
     1081 aaattgatga cgaatggctg gagtggtttc tactttcagt gctgtgtact aagcctggta
     1141 ttcctgacgg ttagctggtt gttgagtggt gaatggttag caggaaagag taaggctgaa
     1201 ccctcacgtt caaccttact ctcatttaca cgttacgctt ttttaaagcg ggcaaagaga
     1261 tgttccacta caacgaaaaa gaccggaacg aagtaaattg ccagcactgt tgcggcaaac
     1321 atcccgccca ttacgccggt acctaccgcg ttttgcgcgc cagagccggc accatgactg
     1381 ataaccagcg gcagcacgcc gagaataaag gccagagaag tcatcaggat tgggcgtaaa
     1441 cgcatacgcg ctgcttcgat gattgcctct accggcgttt tcccttcttt ctgcatcatc
     1501 tcaacggcaa attcgacaat caggatggcg ttcttggcgg aaagcccgat ggtggtcagc
     1561 aaaccaacct ggaagtaaac gtcattactt aagccgcgca gatcggtggc cagtaatgcg
     1621 ccaacgacgc ctaacggaac aaccaacatc accgagaacg gaattgacca gctttcataa
     1681 agtgcggcga gggcgaggaa caccacgacc agtgaaatgg catatagcgc gggagcctgg
     1741 tttgaggata acgcttcctg atacgacagt ccggtccatg agtagccgac gcctgccgga
     1801 agttttgcga ccaggtctgc cataaatttc atggcgtcac cggtactttt cccggcagcc
     1861 gcttcaccta aaatctccat tgacgggatg ccgttgtagc gttccagtcg cggtgaacca
     1921 taggtccatt cggtagacga ataggcagaa agcggtgcca tcgtgccaga ggcgttgcgt
     1981 acataccatt ggttgatgtt atccggcaac atacggaacg gcgtgcctgc ctggacatac
     2041 acttttttca cccgcccctg gttgaggaag tcgttcacgt agctgctgcc gaaggcggtg
     2101 gaaattgtct ggttgatatc agacagcgcc acacccatag cctcggcttt cgcagcgttg
     2161 acgttcactt tgaacatcgg cgtatcttcc aggccgttcg ggcgtacccc gatgacctga
     2221 ttcggtgatt gcgctgccag tgataacagc tcgtttcgcg cctgggtcag cttttcgtgc
     2281 cccagattgc cgttgtccag cagttccata tcaaaacctg acgcggtacc cagttcagcc
     2341 accgcgggta agttgaacgg gaagacgacg gctttattga tactgcttag cgcaatcatt
     2401 gcccgctgaa taatcgcggt aaccgagttt tcctcgccga cacgttcaga ccacggcttg
     2461 aggctgataa acgccagacc gttgttttgc ccctgaccac tgaagccaaa gccaccaacg
     2521 gtaaacaccg actgaacatt atttttctct tttgtcagat aataatccgt cacctgttgt
     2581 agcactttcg tggtgttaac catggttgca ccggaaggta actgcgcggt ggtcataaat
     2641 accccctgat cctcttctgg taagaaagag gtcggcgtac gcaggaacag caccgccatc
     2701 ccggcgcaaa tcagcaggta gaccaccatg tagcgaccgg tacaacgcaa cagcgagcgg
     2761 gtgctgtcgg tatagtgctg agttgatttt tcaaacagcg tgttgaagcg tgcgaacagg
     2821 gcgttaggtt tgtgaccgcc ctccggcgcg gctttcagaa tggtggcgca cagggcaggg
     2881 gtcaggctca ttgctacaaa tactgaaagc agcatggagg agatcagcgt gatggagaac
     2941 tggcggtaga tttccccggt cgcaccgctc ataaaggcca tcggcataaa cactgcggaa
     3001 agaacaacgg caatcccgac cagcgcacgt tggatctgcc ccatcgattt atgcgtcgct
     3061 tccttcggcg gtagcttatc ttccgcaatg acacgctcga cgttctccac caccacgatg
     3121 gcgtcatcca ccagtaaccc tatcgccagc accatcccga acatagttaa cgtgttgatg
     3181 gtgaaaccga ccgccgacaa gatcgcaaac gtcccgagaa taaccaccgg tacggcaatc
     3241 gtcgggatga ttgtggcgcg gaaattctgc aaaaacagat acatgaccag gaagacgagg
     3301 atgatagcct caaccagcgt tttgaaaact tcctgaatag aaattttgat aaacggtgtg
     3361 gtgtcgtaag gataaaccgt cttcaggctt gcagggaaat aagccgataa acggttcagc
     3421 tcctctttca ccgctcgcga ggtatccagg gcgtttgctc cggtagccag tttgatggcg
     3481 atcccggcag caggtttgcc gttatagcgc gccacggtgg aataatcttc cgccccaagt
     3541 tcgacgcggg cgacatcacg cagcagcact tgcgaaccat cttgctgaac tttcaacagg
     3601 attttgccaa attcttccgg cgtttgcaga cgcgtctgca caatgatcga ggcgttcagt
     3661 tgctggtctg ccgcctgtgg catgccaccc agttgaccac cggaaatctg gttgttttgc
     3721 accttaatct gggaaataac atcggaaggt accaggttat atttattgag tttttgcggg
     3781 tccagccaga tacgcatggc gtactcggaa ccaaagagct gtacgctacc aacgcccgcg
     3841 gtacggctta gcgggtcttt gatattagac gctacatagt ccgcgatatc gtactggttg
     3901 aggctgccgt tatcagaaat aaatgccgct accatcagga tattactgct cgacttatcg
     3961 acgctaattc cctgctgctg caccgcttca ggtaatgaag gcatagcgag ttgcagttta
     4021 ttttgcacct gaacctgtgc gatatcaggt gatgtccccg tctcgaaggt cagagtgatg
     4081 gaggcattac ctgccgcatc gctggttgaa gacatgtaca tcaggccatc aagcccattc
     4141 atattttgct caatcacctg agtgaccgag tcttctaccg tttgcgcatc ggcacccgga
     4201 taggtagcgc tgatggtaat ggtcggtggc gcaatctgcg gatactgcgc aaccggtaag
     4261 ttcatgatcg ccagaccgcc tgcaagcatc ataataatgg caagtaccca ggcaaaaacc
     4321 gggcgatcaa taaaatagtt agccatgaaa gtccccttaa gcctgcaacg ttattgtttc
     4381 gattcggtgc tggcgttttc ctggctggag gaaattgcac gtgctttgat acccggacga
     4441 atgcgttgca aaccggaaac gatcacccga tcgccagcct gcaagccaga ggtgacaacc
     4501 cactggtcgc cgatggcttt gctggcttcg atttcgcgta gctgcacgac atcgtcttta
     4561 tccagaatga gtgccgttgc tttaccctgg gcgttgtggg tgacgccttc ctgcggcacc
     4621 agtaatacat tctggcggct accttcatcc actaatgccg tgacatacat gccaggcagc
     4681 aagtcaccat ttgggttggg gaaaatcgcc cgtaacgtca cggagcccgt ggtttcatcc
     4741 accgtcgggt cggagaattt cagcgtgccg gtctggccgt aacgtttacc attttccaga
     4801 ttgagctgta ctggcgtact gccctgaacc tgtttgattt gcccactggc gacctcttct
     4861 ttcatgcgca agaaatcctg caccgactgc gtgagatcga cataaatcgg gtccagacgt
     4921 tgtacggtaa ccagcgaatc tgcctgatta gcggtaacga gtgcgccgac ggtcaccgac
     4981 gatttcccgc tgacgcccgt aatcggcgag gtgacattcg cgtattgcag attgatcgtc
     5041 gcctgttcaa cagccgcttt ggcgacggtg acattggctt ctgcttcatt caactgggtg
     5101 cgtgcggtgt cgtaatcctg acggctaaca tagttggtct tcagcaacga tgcctggcgg
     5161 ttaaaggtga tgcgggcatt gctggcggta gagagcgctt tcgccagcga acctttggcg
     5221 gagttcagct ctgcctgtaa aggcgcagga tcaatctgat acagcgaatc gccctggttc
     5281 actttatcgc cttcgataaa gttgcgttta atgataatac cgcccacctg gggacgtatc
     5341 tcggcaactt cataaggaac ggttctaccg ggcaattcgc tcaacacatt gaccgaaccg
     5401 ggggagagtg tgacgacacc gacctcaggc gtcatggcgg cggcgttttc cgccgatttg
     5461 tcatcgcagg cggtgagcat cgcgccgcag aataacaacg gtattaacag ttttcttctt
     5521 ctgttcattt tagtccctga aaattcttga gaaataacgg ttctttaaat tgcgcgcgag
     5581 gagagatatg agggatttac agaccagcgg catagtgggg ttgtcagcca gtaagatggg
     5641 atgcaagcac agtgatcgac atggtgaggt caacgacaaa aaaaatgttg ccgttctgcc
     5701 aacagtttct gccagcattc gggcaacgaa gggttctact ggtggataca cataccaggg
     5761 gaataaagtc gttttcgtct ctccttcaat aatcaccggg ggtattgaaa tcagcatctg
     5821 catccctcgt catgccagcc atcaatttca gttgcttatg tccttactaa aaataagatg
     5881 tgatacccag ggtgacgatg tcgctcatac gtcttaattt cagcttgtac ataatgttga
     5941 atttctgaat ctttaatgtt tgttgcgaaa tgttgagcgt tgacgtgata ttgctttcat
     6001 ttttgttttt cagaatcagt tgcataattt tgaactgatt atgtgaaaaa catagttgtg
     6061 aatttttatg gggcaagtgt ttaccataag taatctcaag aagatttttg atactttctt
     6121 tacggctgac aaaataaata ggcgctttag cttttagttt actgatgtga taaggtgcca
     6181 aaacaataat tctctcggca tctaatttct ccaggaattt aatcgcttct tcatcaagga
     6241 tatggttgtg ataggtgtca atgatcacct tgaagggttt attgcctact tttttgatct
     6301 ctgacaggga attgattttt atcatttcgt gattatcttt caactgccaa aagccctgta
     6361 aaagaaaaga atctttcgtc atgagaaaaa tcataacttg ctccttagcc gttatcgttt
     6421 cgaacatgtc atccttttca ttatttacat ccttgtccga atcgttgttc aatatagtaa
     6481 acgcctataa gaactttgcc cttcgtgaac taatacgtct attaacacta atcaatactt
     6541 cagatttatc catcacacac tccatatatt ggtcctaagt aagattcctg gttgttatca
     6601 gtttgtaact agtaaacaaa aggttaactt ttgcggatta ctgcccaaga ataagttatc
     6661 aataaccagt ttgtgatctc tgaagaatat tactaaagtt aaaatgtaat ccgatttaaa
     6721 tatcgagtct ccttgtttcg acttaggctg gcaattggat tgccagcttt cttttaacgc
     6781 attgaaatta cctgattaaa taactcattc atgtcttcag gtttttatat agcgtctgga
     6841 gacacggata tttatgcaat gtaatcaacg ccagttgcaa aaaaatgaac ccggattact
     6901 tatttaaccg cccatagcat caacgcttac ggaactcttt gttcactttc ccacagcact
     6961 tttaaaacat taaacctgac cacggaaaat atcagccatg ataatgtttg aaatggctaa
     7021 tttgccatgg aacgaaaaaa atcagattaa attcagtagg ttgaaataat caacagcagg
     7081 caattgtttc aatgatagtg cgtaattgaa ctgaaacact gtatagctaa gagagtatta
     7141 actggatcat agtcacttaa tgtgactatg atccaggtgg aaatagggat aattattgct
     7201 ttttaacgaa caaactggcg aagctgaaca gactggcggc gctaaatatc agttcaattc
     7261 ccaccagtgt ggaaaccagc gttaccgaca ccatcggcgt agcaccaagg aatatccagg
     7321 caatgacgat atccagcaca ccaataacga gctgtagcca actgcctttc attgaacgct
     7381 gacgatacca actcatcagg cgaataaccc ctgccacaca gaacaaaccg gcaataaatg
     7441 ccgcaatggc aaaaatgccc agctctggtg cgcggatgaa gaaatagccg atcaataaat
     7501 aggcgactgc gacgaggaaa ccggataata ccggccagaa attatgactg cggttgctga
     7561 atagcccgac aataagcgca atgcccgagc agattaataa tgcacccact actgtgctta
     7621 aaatgtcgcc agagacgaac ggaaaactga tacacagcaa cccgacgata aacagcagca
     7681 cggcaataaa ctggattgct ctgcgatgtt ttttaagcat ctccagatca aacttcaaaa
     7741 ttgttgcctt atctatatat aacatagaac caccctataa aattaagaag aaaatcccct
     7801 gctatcaatc tatgccaaaa acgcgtgtaa gaatgcagtc gatttaataa aaatttccta
     7861 attacagtat ctgatgtatc tgtaactcat tgtattgaaa taaaaatatc tgattttgat
     7921 attttccatc aacatgacat atacagaaaa ccaggttata acctcagtgt cgaaattgat
     7981 tcgtgacggc tctttcactt tatagttgag gatattacga tgaaaaaagt attaggcgtt
     8041 attcttggtg gtctgcttct tctgccagta gtgagcaatg cagcggatgc acaaaaagca
     8101 gctgataaca aaaaaccggt caactcctgg acctgtgaag atttcctggc tgtggacgaa
     8161 tccttccagc caactgcagt tggttttgct gaagcactga acaacaaaga taaaccagaa
     8221 gatgcggttt tagatgttca gggtattgca accgtaaccc cagctatcgt tcaggcttgt
     8281 actcaggata aaaaagccag ctttaaagat aaagttaaag gcgaatggga caaaattaag
     8341 aaagatatgt aattcctgga atgcgttaca tcgtatttcc ttgcatattg aacaggccgg
     8401 aatatcttct ttaaaagcag ctattcctcc tgtttgtata taatctctat attgaatggg
     8461 ttacaaaatg aacatttcat ctctccgtaa agcgtttatt tttatgggcg ctgtagcggc
     8521 tttgtcactg gtgaacgcac aatctgcgct ggcagccaat gaatccgcta aagatatgac
     8581 ctgccaggaa tttattgatc tgaatccaaa agcaatgaca ccagttgcgt ggtggatgct
     8641 gcatgaagaa acagtatata aaggtggcga taccgttact ttaaatgaaa ccgatctcac
     8701 tcaaattcct aaagtgatcg aatactgcaa gaaaaacccg cagaaaaatt tgtatacctt
     8761 caaaaatcaa gcatctaatg acttgccgaa ttaataaggt acaagtaaaa aggagtagca
     8821 agttgagcca tcttgctgct cctttttgca tttttatatg acagcagaat ttattatacg
     8881 acttatactt gcggtaattg cctgtggcgc tattggcatg gaaaggcaaa tgcgcggcaa
     8941 aggagcaggg ttacgcacac atgtattaat tggcatggga agcgccctgt ttatgattgt
     9001 ttcgaaatat ggttttgctg acgtgctgtc tttaggtcac gtcggactgg atcccagccg
     9061 tattgctgct caggttgtga cgggcgtcgg gtttatcggt gcaggtaaca ttctcgttcg
     9121 taaccaaaat attgtaggtc tgacgacggc agcggatatc tgggtgaccg ccgccattgg
     9181 tatggttatt ggcagcggta tgtacgaact gggtatttat ggttcagtga tgaccttgct
     9241 ggtgctggaa gtcttccatc aattaacctt ccgcctgatg aacaaaaatt accatttaca
     9301 gctgacatta gtgaacggta atacggtatc aatgctcgac tggtttaagc agcaaaaaat
     9361 aaaaacggat ttagtttcgt tgcaggaaaa tgaagatcat gaagtggtcg ccattgatat
     9421 ccagcttcat gccacgacgt cgatagaaga tctgttgcga ttattaaaag gtatggcagg
     9481 ggtgaaaggc gtttcaatta gctaatctgt atagttattt attcgcagga gtaccagtgc
     9541 ggtactcctg ttggttttaa tgatcgagga cgatcagtgg tgaatggttg gcaggatctt
     9601 taagtacggt aatatccgcg ccgtagagca ttgttagcgc ctgctgtgtc aatacgtcct
     9661 gcggcttacc atttgcggta acctttcctt tttgcattaa tacaacacgg tctgcataac
     9721 gcgcggcaag atttagatcg tgcaatacgc agcaaacatt gaactgccgt tcgtgaacta
     9781 actggcgtaa taaacgaaac aagtgctgct gatggtgaat atccagcgcc gaagttggtt
     9841 catcaagaaa taaccatttc ggtgagggag taggttccca gagttgcacc agcaggcgcg
     9901 ctaattgcac gcgctgttgt tcaccaccgg ataattgccg atagtcgcgg tttgctaacg
     9961 cctgacagtc acataacgcc attatttgtg ctgtttcatt atcctgatta ccagtacgat
    10021 gcggatgccg ccccatctgg ataacttctt gtacgctgaa tggaaacgcc atgtggctat
    10081 tttgacgcat cacggcccgg tgtttcgcca gttcagtaat cgaccattca tttaatggct
    10141 tattaaacaa ccgacattcg ccgctatcag gttgaagata gccggttagc tggcgaagca
    10201 gcgtagattt tcccgcacca ttaggcccga ggatggcaac aatttcccca ccgggaaaag
    10261 tcagcgagac attatcagtc agacgccgcc cctgaaggct ataaaccaga ttctgggcgc
    10321 tgatcatgtg atcctccggt tgcgcagaat caaccacata aaataagggc caccaagcag
    10381 gctggttaat aatcccacgg gcatttctgc tggctgtacc agcgtgcggg caagcgtgtc
    10441 tgccatcagc aataaacagg ctccggcgag ggcggaacaa gggattagcc agcggtgatt
    10501 tgccccggta gtcatgcgaa tcagatgcgg gatcaccagg ccaataaagc cgataatgcc
    10561 gcttaccgat acggcagcac caacgagcag tgagctcact aacagtaatt gctggcgttt
    10621 acgcttcacg ttcacgccga ggtagtgggc ttcttcatca ccgagctgca gtaaattcag
    10681 cgttccggcg agacaagttg ttgcgataat ggccggtagg atgaatgaac tggcaaccaa
    10741 taacgtcggc cattgcgcct gtccaagatt gcccatcatc cacaatgtga gttgtcgtag
    10801 ctgctgctca tcgccgatat agctcaggat gccgatcgct gcgccgcaca gggcattgat
    10861 ggcgataccg gcgagtaata attgcatcat gctgccatcg cgatggcgct gcgtgatgag
    10921 aaaaatgatg gtgcagacca ctaaacttcc ggcgatggca aacaccattt gctcataaag
    10981 caccagcacc actgggaagg agaagggcag cacaatagca acgccaacca ttaatgcaga
    11041 accgctactg acgccaagta atccagggtc agccattggg ttgcgaaaaa gcccttgcat
    11101 aattacccct gcggttgcca acgctgcacc taccagcacc gccagcaaaa cccttggtag
    11161 acgaatattg agccagatat cgcggtattc ctcatcaaaa agggcatcaa aactgatctt
    11221 taacgctccc tgactggcac caaacagcac cagagcgagc aataatagtg atagccccat
    11281 aaagaccctg gcggaagaga gtgaatcctt gagcacgcaa aactccgtta cgacattacg
    11341 gcgcggacac gccgcgccgg gggatcacgc cggtttcagg tccggttcaa tcagactgta
    11401 gacctgctgg ttaagtgctg gcgcatttgc cagttcatgg atatgtgcgg cgacatcagc
    11461 acggttaaca aaaccatgac actcctgatt ttgtatccgt tgtgctttgc ccgttgccgc
    11521 accatcaagc agaccgccgg ggcggagaat ggcataatca agctggctgg tttgcaacca
    11581 gctttccgcc agcgtttttt cccgtaccgc ctgaccaaac gcagcctttg cccgctctga
    11641 taaaaacggc cagctatcgc cgcatcccaa agaggtcacc agaatcatgc gactgatgcc
    11701 agccttctcg gcctcatcaa tcactgttct gtgcgccaga taatcctgag ctccgcccat
    11761 tgttgagatg ataagcgcat ccggggcagc tgcgcggcaa gcagccgcga ctacgctggc
    11821 atcacaggcg tcgcctgtaa aaacctgtac gccttgttgc gccagtttcg tggcggcatc
    11881 ggcatgacga atgacagcaa cgaccggacg ttgttccgcc agcgccagtt caagcgttct
    11941 ggcacccaca ccttttccgc ctgcgccaaa gagtagccac ggtgtcatca ggcgtgttcc
    12001 tttaacgatg ctgccagagc atggaaggcg ctgacttgtt cgctcaggag ctgacgatga
    12061 tcatccctac cgaggaagat tttcagcatt gcgctgcctt ccttattgaa aaagagaata
    12121 gaggcggtat ccatgcccat aaatttacgt tcgatcaagg caatatgcgt gcagttttct
    12181 gctttgatat gcccggacat gccgtgcttt ccgcgtaggt tgaaatagcc gtggcgatgg
    12241 aaacccgaag gcagttcgcc gctaaattcg agtattacat cggtagtatg tactaacgtg
    12301 gtgacgttgc cccattcgca gacggtatcc cagacagtat cgaatttatc acctgacaca
    12361 acagtcggag agggcagatt tttcactacc tccagtagcg tggtgttgta ttgttcggcg
    12421 atcgcctcca gcgtaccgtc tggctccgtt ttcaaaaatt cctgtaacga gacatggctc
    12481 ataaatcact cctgttatgg tttttcaagc gcaattgctg gcgcattaag tacctgaatc
    12541 aattcattaa gagactgcaa aatgttgctt gcccagaaac gaccttcatt ggtcaaccgc
    12601 agacacgttg acgcatcgcg gcttaagcct ttttggtgcc actgggccag taacggcgcg
    12661 agtttttcgg catgtggcgt aagttcgtcc agcggtacac gcgccgtttc aatgcccgac
    12721 tgcaaggtat gacgccactg cgcgttacgt tcggcgttac gcatgatcat catcagcggt
    12781 tttttacctg ctgcgacgga ttcatgccag gtctgaagat tgcgttcgtt catccaggag
    12841 taaccattaa tcgaaccgcc ggctccggaa ccaaaggcca gacaatcggc accttgttta
    12901 atcagcaggt tatagagatt gcgttcgcgt gtggtacggc cccagtggct gttactgatg
    12961 cagcgccaac cggcatcgtc cataaaatca cacccttgca ggtaaagatc gcgacgttct
    13021 gccggagagg gcacggtagt acgcccattt tccacggctt tgcccagcgg tgtattgggc
    13081 aggacattga gcgcatagag atcgacgccg tcgagaccga tatcgcgggc aatagccaga
    13141 tcttctcccc aggtttgcgc atcctgaccc ggcagaccaa acagcaggtc acagaccacc
    13201 gcggcacggt cgcgtttgac caggctttcc ataaacgcaa tagcggttgg gccatccgag
    13261 gtgcgggcca ttttcttgcg gattttgctg ttaaacgact gaataccaat tgagaagcgg
    13321 tttgctccag catcaaggca agcatcgatt cgctcagcgt caaaattcag tacccggcct
    13381 tcgatagtga tttcacaatc aggggccagt ggcagctttt ctcgtaatgt ggtgatgatt
    13441 ctggccaaat cgtgtgccga aagcgcggaa ggcgtaccgc caccgaaata gaccgcgtgg
    13501 atgggggccg actgatgcaa tacgctgtct gcttcccgtt ctatttcacg aatcagggcg
    13561 tcggtataat gagcacacgc atcttcatta aaacggttct gataaaaacc acaaaacgtg
    13621 cagtgtgtcg cacaaaaagg aatgtgtagg taaaccagtc gcttacgtgg tgataccgtt
    13681 tgattgatca tctcttgcca ggtctgtgcc agttgctctt tggcaaccgg aatggcccca
    13741 cgaaacggca tcatggcgcg cctgtcttta aagggctgat caccgtctaa agcaaaatgt
    13801 ggcgtgagat ccagggtgtt atttgtgttc attcgtctga tttaaatcca tttattgcgg
    13861 ccaaagctgc tggtgtaaag actcaacgac atcagcaata cgcggaccca ttcccagaat
    13921 caggttttga tcaacggtga taatgcgctg gtttttccat gcggcagtgt gggtaattcc
    13981 ggcaatagaa cgtagccgat taatatcgcc atcgaccatt tgtgaggtga cgacaatcac
    14041 ttcagggtta gccgcaatca gcgattccgc gctgtagctt ttgtactgct ggtgggtagc
    14101 gacgttttct gctccggcaa gcgaaagaat ggcgtccgcg acgctgcctt taccggcaac
    14161 ctgtggcgca cttccgcctg cggagagaat aaacatggct ttaaccgggg cttttttggc
    14221 cgccacgttt tgctgtactc gctccaggcg ttggttgatc tgtgtcacca gcgcctcgcc
    14281 ttgttcagga acctgtaacg tttttgccag ttgacgaatg ttggcgtaca tctgctcaag
    14341 ggtggctggt acacgcggca gagtgacgac gttgactttt tgcgcccgca gttggtcgag
    14401 cacaatttgc ggtcctgcat cctgccaggt aattacgcta tccgggcgaa gtgacaaaat
    14461 gccttcgctg ctgagttgtt tccagtaacc aatatgcggc agtttggcgg tttctggtgg
    14521 ataggatgtc gtttcatcga caccaaccac gcgtttgcca gcgcccatcg cgtagattag
    14581 ctccgtcagc gatcctcctg cgaccacgat acgttcggca gccgttacgc taaaagtcca
    14641 gctaagagcc agtaatgccg taaacaattt ttttcgtttt accatggtct tcgcggcaga
    14701 gaccacggct gaaatacata acgttagtgg ggaaaaaaga aacggcctgg tgatgatcct
    14761 tggcataatg tgagaactct tatcaaattg agaatgatca tgattatcat ttgagttata
    14821 tatgaaatac aaccgtcgat tcaatacgca cggcgttaca agcgtattta tttttgattt
    14881 ttatcaagat gcttcgtcag ctaatgttct tgttaccgat taaatttcag cgcgggaaaa
    14941 agctgagaac ccggagatat cgcggcttgc agagcaaaga gaagagaaaa ttatcactgt
    15001 taacggcaac gtttcgtgat gaggttgtgg ggggatagcc ataaacacag gatggttttc
    15061 tggatgctgg atagatttca taaccccctg tttattgtgt taatggcggt taaaaaaagc
    15121 ttgatcataa aaaattgatc gataataatt ctcattatca cgtttgagtt taacaaaata
    15181 tgacatcatg gataacaaaa tcatatct
//
LOCUS       DADSZD010000105        15185 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.106, whole genome shotgun sequence.
ACCESSION   DADSZD010000105 DADSZD010000000
VERSION     DADSZD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.106"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            209..1522
                     /gene="gltP"
                     /locus_tag="J1Z28_003004"
     CDS             209..1522
                     /gene="gltP"
                     /locus_tag="J1Z28_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBA7717818.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(1670..2359)
                     /locus_tag="J1Z28_003005"
     CDS             complement(1670..2359)
                     /locus_tag="J1Z28_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBA7717819.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(2470..3174)
                     /locus_tag="J1Z28_003006"
     CDS             complement(2470..3174)
                     /locus_tag="J1Z28_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7717820.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCGKST
                     ILRVIAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            complement(3161..3997)
                     /locus_tag="J1Z28_003007"
     CDS             complement(3161..3997)
                     /locus_tag="J1Z28_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7717821.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            complement(3990..4823)
                     /locus_tag="J1Z28_003008"
     CDS             complement(3990..4823)
                     /locus_tag="J1Z28_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7717822.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            complement(4823..5872)
                     /locus_tag="J1Z28_003009"
     CDS             complement(4823..5872)
                     /locus_tag="J1Z28_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7717823.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTGQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            complement(5972..7540)
                     /locus_tag="J1Z28_003010"
     CDS             complement(5972..7540)
                     /locus_tag="J1Z28_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7717824.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NASSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSTKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            complement(7915..10062)
                     /locus_tag="J1Z28_003011"
     CDS             complement(7915..10062)
                     /locus_tag="J1Z28_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(9643..9645),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HBA7717825.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(10260..11726)
                     /gene="mdtP"
                     /locus_tag="J1Z28_003012"
     CDS             complement(10260..11726)
                     /gene="mdtP"
                     /locus_tag="J1Z28_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBA7717826.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQFNDPQLDALIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQVLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQTAPVVEKK"
     gene            complement(11723..13774)
                     /gene="mdtO"
                     /locus_tag="J1Z28_003013"
     CDS             complement(11723..13774)
                     /gene="mdtO"
                     /locus_tag="J1Z28_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBA7717827.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIASLFLIYKWSYGEP
                     LIRLIIAGPILMSCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEAVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPISFADSQAIIEFRQKL
                     ASEINKLQHSITEGQCWQSDWRISESEAMTARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(13774..14805)
                     /gene="mdtN"
                     /locus_tag="J1Z28_003014"
     CDS             complement(13774..14805)
                     /gene="mdtN"
                     /locus_tag="J1Z28_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBA7717828.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(14824..15099)
                     /locus_tag="J1Z28_003015"
     CDS             complement(14824..15099)
                     /locus_tag="J1Z28_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717829.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPMIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         3556 a         3988 c         4021 g         3620 t
ORIGIN      
        1 ttaatcctgc cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa
       61 agggttgcgc aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa
      121 ccaacgcaac acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga
      181 acggcgctcc attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca
      241 gattctgttt gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga
      301 cagccgcgac tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct
      361 gatcaaaatg attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt
      421 tggtgatgcc aaacagctcg ggcgtattgg tgcgaaaacc attatctact tcgaggtgat
      481 caccaccgtc gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg
      541 ggtggatatg tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ccacggaagc
      601 ggtacaaagc agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat
      661 cgtggcgtcg atggcgaaag gcgaaatgct gccgatcatc ttcttctcgg tgctgtttgg
      721 tctggggctt tcatccctgc ccgcgacgca tcgtgaaccg ctggtgacag ttttccgctc
      781 catctctgaa accatgttta aagtgactca catggtgatg cgttatgcgc cggtgggtgt
      841 gtttgcgctg attgcggtga cggtggctaa ctttggtttc tcgtctctgt ggccactggc
      901 gaaactggtg ctgctggtgc atttcgccat tctgttcttc gcgctggtcg tactgggaat
      961 tgtggcgcgc ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct
     1021 gattctggcg tactccactg ccagctctga aagcgtgctg ccgcgaatta ttgagaagat
     1081 ggaagcctac ggagcaccgg cgtcgatcac cagtttcgtg gtgccgaccg gttactcttt
     1141 taaccttgat ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta
     1201 tggtattgac ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc
     1261 gaaagggatt gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag
     1321 cgtaggtatt ccgctggaag gtctggcgtt tattgccggt gttgaccgta tcctcgatat
     1381 ggcgcgtact gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg
     1441 ggaacacaaa tttgaccgta agaaagcgct ggcttatgag cgtgaagtgc tgggcaaatt
     1501 tgataagact gcggatcagt aattgaagat tgccgggggg atatccaccc ggcaatgtgt
     1561 ggatgcttga ctgagtaata gcctgatgta gcgtggtttt tgtaggtcgg ataaggcgtt
     1621 tacgccgcat ccgacatcaa cgcctgatgc gacgtttgct gcgcctacct taaccgttgg
     1681 ttaatttttc aaactcttca cacccggtat caaacccttc catacagctc aggttcaacc
     1741 agtgcagcgc cttttgtttg ttcttctcga taaaaccctc ttcaccgttt aagaacatca
     1801 ttcccgccca gtattcggaa taaccggttc gggaaatcgc agagctgcgt ttgaaatacc
     1861 aggttgcttt gtcatcgtcg gccttaatgc ccacgccgtt ggcgtaaatc aaaccaagca
     1921 gcatttgggc atcgacggca gagtcgttct ccagatcttc cgaggcgttt tctaacagcg
     1981 aaatcgcctt tggataatcc ggtttacccg cttgcgtatt taccagaata tgcgccagcg
     2041 ttacttcacc ttctttactg cccgcttgcg ctgctttctc ggcaagcact tttgcttgtg
     2101 gatagtccag actgaccgga ttggtgattt tgatctgcgc caacagtgcg caggcatcag
     2161 catcaccgct gtcggcggct ttctgtgccc aatattcggc tttgctcaaa tcgccggagc
     2221 taaaccagct gtcggcaaga aaatattgtg cgcgtcggtc tccggcctct gctgccttta
     2281 aatactggct gccaggctcg gagtcgttcg cgtgggcaaa gaatgtcaaa aacaacatca
     2341 aagtgataat ttttttcatt ttgataatga agtttggtag gggagagaca gtataaagtg
     2401 ttcgggtgga caaaaaaaca actgtaaaaa gacctgaaag cagaatgaat taatgcaatg
     2461 tcaggttttt taatccatcc gatgttcccc cttttccatg gcgtcgcgat caaaaaatcg
     2521 ctgaattttt ccctcggaca tcaatgccgc tcgatcagac atatgcgcga tgacatcggc
     2581 atcgtgactg accagcagat atgtcatttg gtgttccagc ttaagttgat tcaacaaatt
     2641 aaggatttcc gcttgtaccg acatatccaa cgccgaggtg ggttcatcca gcagaagaag
     2701 ttgtggtcgt aacaacaggg cgcgagcaat aaccacacgt tgccgttgac caccggaaag
     2761 ctggtgtggg tagcgacgag cggcgtcgaa aggcaagcca acctgttcca gtgcagtctg
     2821 gacgcgtttt tctatttctc tttcgccacg aattttgagt ggttcagcca gcgttcgcca
     2881 cagagtatga ttgggatgca acgaggcata gggatcctga aacaccattt gcacattgcg
     2941 gcggagatcg ccctgaaaac gcattcctgg tttgatggtg tgcccaaaca gtgacacctg
     3001 accgtgccag tcgcgctgta atccagcaat tacgcgcaaa atcgtggatt ttccgcagcc
     3061 tgattcgcca attaaactaa atgtctctcc ggcattaacc gaaaaactgg cggcagaaac
     3121 tgcggttttc tcagcaaaag tcacctgtaa gtgattaatt tcaacaatcg tcatggtatt
     3181 tctccggcgt cattgccgtc ctgtccagcg tcggcagcat ctgcccgtaa gtttgtgcat
     3241 ttgggcggca agtccacagt gtgcgcgtgt aaggatgcgt tgctgtaggt agcgcagcag
     3301 cgtgcatttc atccacctta tttccctgat acatcaccag cacccgatgg caaaattggg
     3361 caactaatgg caggtcgtga ctgatcagca acattgccat ctgacgttgt tcacactggg
     3421 taaccagcag ttccagaatc tgattacgca ggcgcacatc cagtgctgat gttggctcat
     3481 cggcaatcaa tacccgggga tcgttgatga gcgcaattgc aatcatcact cgctgcccca
     3541 tgccgccaga aagttcacga ggatagcttt gcagtactcg cgtattgagg ccgacagcct
     3601 gaattgcggt gtttattttc tcttctttct cgcggcggtt cagacgttga tggagcgtaa
     3661 gcgcctcttc cagttgcgct tttactgtct ttactggatt aagcgcgtaa cgcggatctt
     3721 gcaataccat cgcgatatcg ttaccgcgca actgttgcca tccacgcgcg ttaagggtga
     3781 gtgcatcacg ccccagaatg ttaagtgtgt ccgcggttac tacacccggt ttacgcacca
     3841 gccccatcag ggcgcgggcg gtcatggact tacctgaacc cgactcaccg accagcgcca
     3901 gacgttcatt gcctaatgta aaactgacat tgttgacgac ccgggactcc ggataatcga
     3961 cgcagagatt gttgatacta agacgtgagt cagtcatgtt gaggctccag aacatcgcgc
     4021 aagccatcac ccagcagatt aaaggccaga ctggcaatta agataattgc gccgggagcg
     4081 gcggcaatcc accattgatc aaaaatgacc tgcataccgt cggcgatcat ggctccccat
     4141 tccgccattg gcgggcgagc accaagccca agaaaaccca ggccagcagc cgccagaatg
     4201 atacccgcta aatccagcgc cagacggaca atggcagatg gcaggcacaa tggtaaaata
     4261 tgaccagcca gcagacgcct gccgcggatg cccatcattt ctgcggcggc aagataatca
     4321 ctgtgtcgca gacgttggat ctcactccgt gcctgacgcg cataagcagg ccaggtggtt
     4381 aatgccaggg ccagtgcgcc gttaaccagc cccggcccca gcatggcaac aaaagcaaaa
     4441 gccagaatta aacgcggcat tgacattact acgtcagtaa aacgcatcag cacgcgttcc
     4501 agccagccgc cgtagtaccc ggctaaaatg ccaatcagca gaccgaccgg gagtgtaatg
     4561 attgtcacca atgccaccag ccccagtgcc gggcggctgc cgtagattaa acgtgaaagt
     4621 aggtctcgcc cgtaactgtc agtgccgagc cagtgctgac gactcggtgc ttgtaaccgc
     4681 gccgccgcat cctgccagtt aggatcgtga ggtgccagcc acggcgcgaa gagcgcaatt
     4741 agcatcaata acaggataat tatcaacccg caaaacgcgg cgggtgaatg gcgtaaacgg
     4801 cgtaaaaaga gaaacatcgg catcagcgta acctcggatc ggtcagccgg accagcaggt
     4861 cggttaagtt atttatcaac acaaagcaga cgccaatcag cagcgttcct cccatgattg
     4921 ctgtggtatc acccgcgaag agcgctgtag tgaggtagcg cccaatacct ggccatgaaa
     4981 agacggtttc tgtcagtacc gcgccttcca gcattgatgt ataggcgagg gcgataacgg
     5041 tgagcaaggt gccgcgaatg ttgggtaaaa catgacgtag caaaattgtc atctcgctgg
     5101 cccctttggc gcgcgccagc agaatatatt ctttattcat ttcgccaaga caggcggaac
     5161 gagtcaggcg cgtaatactg gcgagcgaga agtacgccag taataaaacc ggaagtatca
     5221 aatggttgat tgcattggta aatgcctcgc gatcgccaga aagccacgta tcaatcagcg
     5281 caaatccgct acggggttct atggtgaatt gataaatatc atccagcctg cccggcccgg
     5341 cactccattg cagtctggcg tagaacagcg ccagcatcag cagaccaagc caaaaaacag
     5401 ggacggaatt tcccagtaga gttaatgttc gcacggtgaa atccagcgga gaaccgacat
     5461 aacgggcgca aagtacaccc gcaatgacgc cgacaacagc accgacaata agcgccaacg
     5521 ttgccagttc aatggtcgcc gggaatgctg atagcaagtc ctggagtact ggttgcccgg
     5581 tggagctggc gatgcccaga tcgccgtgtg ccagattcat caggtaatgc cagaactgga
     5641 cggctaacgg ctgatccaat cctaactggt ggcgtacctg atcataggtg gactggctgg
     5701 cgtgatcgcc aacgatttgt agtacgcgat cgaccggtga gagagccgaa agcgcaaagg
     5761 taatcagcag caggccgagt agcgtcagag caacggtaaa cagcccttgc aacagcgcga
     5821 gcagagggcg cttgcctcgc catctcgcgc gagtggaaaa aacgacagac atgatctttc
     5881 catacagtga taaatcagcg gctggcctgc aatacaggcc agccctggta ccgcgcccgg
     5941 taacatggct ggacgcgagt tgcatacagg gttatttggt tacctgatca aaccagacca
     6001 tatcggcatt caacccttgt tgatacccct gcacattatc acgcatgaca atttgcgttt
     6061 tgccttgatc aataaagaca tacggggaac tacgttgtag ttcttgttgc attttcttat
     6121 acaaatcaag acgtttagtg ctatcaggtt cagcgatggc cgccagcgtt tctttgttca
     6181 gttccgggat tttccagccg tttaacccgg caacggtgct ggatttgcca tcgttatagg
     6241 cgaaagagct ggcgttggaa tgtgcgtcaa aataatcagg caaccacata cgaatggccc
     6301 cctgatgctg gtgggcgcga acgcgagagt agacctgact ccctgcggct ggcagcaaat
     6361 caacttttac accgccctga gcaaagctgg cctgaattga ctgcgcgatg gtaataaatg
     6421 gcggtttatt ttcgacatca agcgtgaagt gcgggtcttt gattccggct ttagcgagga
     6481 tctcttttgc tttggctgga ttaaaggtaa acggtgtttc ttccagtgct ccaggaaagc
     6541 ccacgggcag gaaactttga tgggtaaaat attgtccttt taagagattt ttggtgatgc
     6601 cgtcgtaatc gaccaaccag cgagcggcct cccagaacgc aggattactc atcagcgggt
     6661 tatccttgtt tccggtattg aaggccaggt agttctgttc ggcggaggca atgctcatca
     6721 cttttacgcc aggtttaccc tgtaatgcat cgatttgatc ggctcccagg tcacgagcca
     6781 tatccgcatc accttgttgg atcagcaggc gacgtgttgc cggatcggga acatttttga
     6841 tgatgacgct tttcagtttc ggggcgccgg ttggtgagct ggcgttggct tccatcacga
     6901 tggcctgatg cggttggtaa gtacgcattt taaacgcgcc gctgccagcg gaatgcatct
     6961 tcagccattc attaccaaag tcgttatttt tggcgtttgg cgcaaccagc ttctcgtcaa
     7021 caattgaggc gattggtgtg gagagaatat tcagtgccag cgccgggctg acgtcggcac
     7081 tccattgtac ctggacggta tggtcatcga ttttcttcaa ctggctggcg atattttccg
     7141 gctgccatcc cagcacattg agaataaacg caccggattt attcagtgtg acagcccgga
     7201 cataagagta aatcacatct tccgggcgta ccggattacc agaggcaaat ttcgcatcgc
     7261 ttttcagttt tatggttagc gttttggctg cggggtcagc ctgccaactt tccgccagaa
     7321 tcggagtgat tttcgtcgga tcattacggt ccggttgtac tacgcgttga tacagacttg
     7381 gtacagtctg aatacttgag agttcattag cttctgccgg gtccagactc acaatatcat
     7441 ccagcccctg ggctacaacc aacgtattag gcggtgtagc ggcaacggtt gtggcggaaa
     7501 ttgtgcttaa aaccagtaaa ggtaatagtt ttttaatcat atgcttccca atccctgaaa
     7561 ttactttgag gttattaata tgctatcaga cggggtttcg ctgcattaat cgcgccagaa
     7621 tatcccgtta atagaacgag ttaaacggtc gatgatttct caccttaccg aaattttgtt
     7681 tatcaaatgt aaagtattat tacgttaggc cgcgatgaga attgagtaaa gataattctc
     7741 atttgcaatc gtgactggaa tagaagaaca accgagaaga cgtcgctaat aaccaggatg
     7801 ggctggtttg actgcgcagg tatgtataaa aaaatccgga aacagagagt tgtttccgga
     7861 ttgatacaaa ggcgttgggt aatatcgccg cttttaacgt gagtttacgc ggtattacgc
     7921 cagtgccgct tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc
     7981 ggcggcgcgc tgatcggcga tcggctcgac gcgcacggcg cagtatttgt attccggcgt
     8041 tttcgtaatc gggcttaagt tttcggtcac cagctcgtta caggcaccaa tccaccactg
     8101 gtaggtcatg taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat
     8161 tttgccttta cgcgagtgca cccaaaccaa tgcctcatct tcaataccca gacgtttggc
     8221 gtcttcggta ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca
     8281 gttaccggtc atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat
     8341 cgggtattcg tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgcgc
     8401 cagaccgttc ggggtatcaa acttctcttt aaacagataa gaagtcccct gatcggcatc
     8461 tgaagtatcg cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc
     8521 accgtagaaa tccgggcaca gatgacgcaa ctcgtcccag atctcctggg tgttgttgta
     8581 gtgcatcgga taacccatac gagtggcaat ttcactgatg atttgccagt ccgttttcag
     8641 atcccacttc ggctcaaccg ccttgaagaa acgctggaag ccacggtcag ccgcagtaaa
     8701 cacgccttca tgctcgcccc acgacgttga cggtaaaatg acatccgccg ccgacgcggt
     8761 tttggtcata aagatgtcct gaacgataac cagttccaga tcttcaaagg ctttacgtac
     8821 tgccgacagt tccgcgtcag tttgcagcgg atcttcgccc ataatgtacg ccgctctcac
     8881 ttcaccatgc gccacgcggt gcggcagctc gctgatgcga tagccggtat gagcaggcag
     8941 gctttccacg ccccaggctt tggcgaattt ctcgcggtta gccggatctt tcacgtactg
     9001 atagcccgga tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg
     9061 accacgaacc gggtttacac ccgcatgtgg cttaccgagg ttaccggtca gcatcgcgag
     9121 gctggtcaga gaacgcacgg tttccacgcc ctgatagaac tgggttaccc ccatgcccca
     9181 caggatggcg gcgctttctg cctgggcata catccgcgcc gcctgacgaa tctcactggc
     9241 gctgacgccg gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata
     9301 ctcttcaaag ccttctgtac gggaagcgac gaacgctttg tcgtacagat tttcttcaat
     9361 aatgacatgg cccatcgcat tcaacagcgc gatgttcgag ccgtttttca atgcaatgtg
     9421 catgtcagca atgcgcgcgg tttcaatttt acgcggatcg cagacgatga ttttcgcccc
     9481 gttacgttta gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttata
     9541 cccgaaaacg aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc
     9601 attaccgacc gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca
     9661 gtcaacgtta ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc
     9721 gttacccgta ccgcgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt
     9781 gatggcgctc aggcgctcgg caacgtaatt cagtgcctca tcccaggaaa caggttcgag
     9841 tttgccgcca cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt
     9901 atcgttaatg aagtcccagc cataataacc cttcagacac agggtaccct ggttggtttt
     9961 cccctgcgct gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca
    10021 acctgatgcg caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa
    10081 tcaaatcacg catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg
    10141 gggtcattcc ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt
    10201 cgtcagtttt gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg gtagacattt
    10261 tattttttct cgacgacggg agccgtctga tacccgccgc ccagcgattt catcaactga
    10321 atgctttgga tcacccggcg gctgtccagc atcagtaatg acatctcctc ggcaagcact
    10381 ggcaaccggg cttcggtggc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg
    10441 gctgcgcgct gggtgaagcg cgttgcttct acgcgttcag cctgcatctc tcgctcgtcg
    10501 ttgagcgttt gcagacgtgt gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac
    10561 tggttgtaac gttcaatcat catgttgctg gcggcgcgag tgccttcgag attggcattc
    10621 aaccgtccac cgtcaaacag cggcaatttc agacccggga tgaagttgaa ctggcgactg
    10681 gtttttttga ataaggtatc caggtggatg gagtccagac cgaaaaacgc tttgatatca
    10741 aagctcgggt agaacagcgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac
    10801 cagcgcatgg cttgcagatc cgggcgtctg gcgagcaact cataagagag cgttgccgga
    10861 atgccggtct gtactcgtgg taatgccacc ggtttgatct ccggcatatc gctggctccc
    10921 gcgccaatca atgcacgcag agattctcgc gtttcagtga tttgcccttt gacggcagca
    10981 atttgtttat cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgcgcttcc
    11041 agaccgtgcg ccactttact ctggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt
    11101 tgttctaaca gatcgagcac ctgatagctg gcctgcatac tgtaataaag ctgcgctacg
    11161 cccgtggtca gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca
    11221 atggcggcag caaccgctga gcgatgcaca ccccacaaat cgagatccag tccggcaaac
    11281 aaaccgactg tggcttccgt atagtacggc ccgtccatac ccagtgctgg cgcatccatc
    11341 gcataagggc ttaaaaagcc gttcgccgag acgcgttggc ggttgagcat ccctaacgct
    11401 gcgacctgta attgtgaacc ggcatctaac aaatcggcct gggactgcgc tttttcttcc
    11461 cgcagtttcg cttcggcgag ggggtgtgaa ccacttagcg tccgttggat cagcgcatcc
    11521 agctgcgggt cgttaaattg tttccaccac tgcgcctgcg gccagccgga gctggcaaga
    11581 tgaatatcat cggccagttt gatttgttcc ggtttgagct gttgatgagg cgcagaatcc
    11641 ttacgtacca gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc
    11701 aggcgtgaaa gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg
    11761 ttaatgccgg ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct
    11821 gttttaataa ggtcggcaga atggcctgcg aggcgggtgt ttcttcaagc gttatttgag
    11881 gagaacggct gagtgcggtt gccagaccgg cggcgtattt ctccagggcg tcggcaaact
    11941 gacctgctct gtccggctgt agtgcgttat ccagcgcctg cgccgagttc caggtggcat
    12001 cccaggcgtg aagaatatcg cggccctgac gaataaccgt ttgcgaacgt tcaatcagta
    12061 atgcgcgttc ttcgctgtcc agttgacgct ccagcaccac gcgttggcac atctcttcac
    12121 aggcattaaa cgccgcatgc agacctatgc gaatttgcag ataagtgcgc agagccgtga
    12181 cttcctgctg gcgtgggatc cgcattactt tactcaacat acccagcgcg ccagccagtt
    12241 tttgcggcag cgtgcgcgct tcgctttcag gccagacaaa ggtgtaaatc accgcggaca
    12301 ccacggtacc aatgatgata cccagagcgc gatcgcgaat ttccaccagg tcgtacaccg
    12361 ggccaaaaac gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat
    12421 aagaagagcg ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca
    12481 caaacagcaa ttcgacaata ttgtccagcc agggcatgac caataacgtg aataacagcg
    12541 ccagaatcgc gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgacgaac
    12601 cgacattggg attagcgacg atcacgcagg tcagcataca ggtgtgaatg ccttcccagt
    12661 ccacgccgct gtagaaggtg taacagatca aacaggcgag cagcgttttt accgcgtagc
    12721 gcatatagtc tggattggta aaggcatcgg cgaccattga tggcggcttg gctgcaggcg
    12781 taggcggcgt atttgggtcc atctgaccaa gctgtaacag cgtctggcag atattctcca
    12841 ggttacattc ccgtgccgtc atcgcttcac tttcactgat ccgccagtcg ctttgccagc
    12901 actgaccttc ggtaatggaa tgctgcagct tgttgatttc tgaagctaat ttttgtcgga
    12961 attcaataat tgcctgagaa tcagcaaaag agatgggatc gtagcgattc agtgtcgagt
    13021 aaatgtaggt taccgttgcc acgcagcttt gccaccatgc gctctgggtt cgccagttgg
    13081 catcgtccgc gaggcaaaag acattgagtt tttgcagcgc cagcgcctct ctttcaatcc
    13141 gcgtttcggg tagcggtgcg aggctgtccg tcaggtggct aatggcatca tcaagccgat
    13201 cattaagcgc ctgatgcatc tgcgtaatgg cacgactggg aaaccacagc acgccgatta
    13261 acgtcatcag caaggttgga tagaggccaa caacgataca ccacagcgtt aagcgcacga
    13321 ccgcttccgg atagtcgagc atggcgggga aggtttgccc gtaaatagcg acaatggcga
    13381 cggcgaaaaa gaccagcccc aaccgatggg tgcgcatcaa aaacatacag ctcatcagga
    13441 tcggcccggc aatgatcaat cggatcaacg gttcgccata tgaccatttg tagatcaaaa
    13501 acaggctggc gatctccagc accgtggcga ccacaaacaa gatcgccaca aatttggtgt
    13561 aaaacgcgtt cgactgaata ccgtaaaaca gcactgccag tgataacgcc acaaaaggga
    13621 tctcaaaagt catcgagatc agaatcacca gcaggcagcc tacccagagt tgcatagtct
    13681 gtggcacgcg ccctggtcgc cgctcgctta actcttcatg aaagaacgcc agcagcctga
    13741 ccaccggtaa tggcagggag ttgagcgcgc tcattattgc ggctcaagat tagcgactgc
    13801 cgaagcgccg atgcggaaca tttccgggtc aggtttatcg accatgattt tgaccggaaa
    13861 acgctgggca acgcggaccc agttaataga acgagacact ttcggcaggc cgcccagcac
    13921 caggccgccg tcatccggta gcacgccgta gccaatcgaa tccactttac cctcgaaggt
    13981 tttgccgctg tcgctcatca ggcgaatggt tgcgagtgta cccgagcgaa tatttttcag
    14041 atcggtttcg cggaagttgg caatcacgta ccagtgacga gtgtcgatta gggtgaaaat
    14101 agggcgcatc gcagatgcaa actgcccgac ggaggttttg agggaaatga cccggccatc
    14161 aaacggcgcg cgaacggtcg ccatttccag atgcagtttc gtcaacgcaa tatccgcttc
    14221 gactgccgca cgttgcgcca ccagcgcatc cacgccgctg acggcgctgg cggctgactg
    14281 cgcctgcaac aatacggcat taaggtccgc ttctgccgca cgctgtgcgg ttcttgcacg
    14341 gtcaacctct tccgcagaaa caaaaccttc tcgcagtagc ggttcggtac ggcgtaacgt
    14401 atcgctggct tgtttcgcgg cggcacgggc tttttctacc gtggcattaa ccgagtcggc
    14461 accaaactgt tgcgcgtcaa cgctacgctg ggtgagcata atttgcttat ccagcgccgc
    14521 gagggaggct tcagctttcg ccagattggc ttcgtacggg cgcgggtcaa tgcggaacag
    14581 caaatcgccc tgtttcaccg cttggttgtc ggtgaccgcc agttcaacaa tgcgaccgct
    14641 gacttccggc accacatcaa tggtatctgc tgacgcgtaa gcgtcattag ttgatggcgc
    14701 actgtctacg cgccagataa cgaaaacaag ggcaaccagc gccaacgcaa ccactaacag
    14761 agcagggaat ttactgcgag gagctttttt cggcgtactt tccatgagcg cgtctcaaat
    14821 agattagaaa aatgccagcc agaacagcat ggcgtagaga gcaaaaaggg cggtataaat
    14881 aattccgaca aatgccagat tgatatttgt ccgctgaata attcgcctcg tgatgagcgt
    14941 caaaataagg ctggcaatag cgcagaaaaa ccagccggga taataagcgc cgatcatcgg
    15001 gattgccgga gataatgagc atcccgaaac catgaagacg ggaattatcc aggctgtttt
    15061 tttgagttgc atcgccatgc gagagagaac tgttggcata ttaaatacat cctgtctgct
    15121 gcgaacaata tctgtagtga atatcaatat ccattgaagg cgtcagtatg gctattttct
    15181 cgatg
//
LOCUS       DADSZD010000106        14955 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.107, whole genome shotgun sequence.
ACCESSION   DADSZD010000106 DADSZD010000000
VERSION     DADSZD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.107"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(19..2298)
                     /gene="maeB"
                     /locus_tag="J1Z28_003016"
     CDS             complement(19..2298)
                     /gene="maeB"
                     /locus_tag="J1Z28_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBA7717830.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            2587..3537
                     /locus_tag="J1Z28_003017"
     CDS             2587..3537
                     /locus_tag="J1Z28_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase A"
                     /protein_id="HBA7717831.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            3557..5560
                     /gene="tkt"
                     /locus_tag="J1Z28_003018"
     CDS             3557..5560
                     /gene="tkt"
                     /locus_tag="J1Z28_003018"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA7717832.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(5637..6680)
                     /locus_tag="J1Z28_003019"
     CDS             complement(5637..6680)
                     /locus_tag="J1Z28_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBA7717833.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGRAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNSGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(6806..7381)
                     /gene="nudK"
                     /locus_tag="J1Z28_003020"
     CDS             complement(6806..7381)
                     /gene="nudK"
                     /locus_tag="J1Z28_003020"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBA7717834.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(7449..9428)
                     /gene="aegA"
                     /locus_tag="J1Z28_003021"
     CDS             complement(7449..9428)
                     /gene="aegA"
                     /locus_tag="J1Z28_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBA7717835.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQRHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFCAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNINAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            9634..11334
                     /gene="narQ"
                     /locus_tag="J1Z28_003022"
     CDS             9634..11334
                     /gene="narQ"
                     /locus_tag="J1Z28_003022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBA7717836.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            11498..14611
                     /gene="acrD"
                     /locus_tag="J1Z28_003023"
     CDS             11498..14611
                     /gene="acrD"
                     /locus_tag="J1Z28_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBA7717837.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNSERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTYEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(14710..14769)
                     /gene="ypfM"
                     /locus_tag="J1Z28_003024"
     CDS             complement(14710..14769)
                     /gene="ypfM"
                     /locus_tag="J1Z28_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBA7717838.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         3496 a         3972 c         3909 g         3578 t
ORIGIN      
        1 gtgagagtta aaaaagaatt acagcggttg ggtttgcgct tcaaccacgg ccagcgccac
       61 catgttgacg atacgacgta ccgatgcaat cggcgttaac acgtgaaccg gtttcgccac
      121 gcccatcagc accggaccga cagtcacacc ttccgagctg gaaacgcgca gtaagttgta
      181 actaatgcgg gcagcttcca tgttcggcat caccagaata ttagcggaac ctttcaaagg
      241 gctgtccggc atacggtcgt tgcgaatcgc ttccaccagc gctgcatcgc cgtgcatttc
      301 accatcaatc atcagttctg gcgcacgttc cctgaccagt tccagcgcct gacgcatttt
      361 gctcgacgac gggcagtcag aagaaccaaa gttggagtgc gacaacaaag ccacacgcgg
      421 ctcaatacca aaacgacgaa cagtttctgc cgccatcaag gtgatctccg ccagctcttc
      481 tgcatcaggc tcatcattga cgtaggtatc ggcaataaag gtgttaccac tcggcaacag
      541 cagcgcgttc attgcacctg cggtgtgaac gccatcgcga taaccaaaga catttttcac
      601 cacgctaaag tgttcatgat aatcgcccac cgtaccgcaa atcattgcat cggcttcgcc
      661 acgctgaacc atgatcgcgc cgatcactgt cgggttactg atcagcgcac gctgcgcctg
      721 ctcctgagtg acaccgcgac gcttcatgat ctggaagtat tcggtccagt actctttaaa
      781 gcgcggatcg gattcgttat tgacgatctc aaaatcaacg cccgccttga tctgcaagcc
      841 cagtttctga atgcgcattt cgatcacgtt cggacgacca ataaggatcg gtttcgccag
      901 tcccagagtc accagttcct gagtcgcgtg cagaacgcgc gcctcttccc cttccggcag
      961 aactacgcgc ttcggcgctt tgcgagcctg ggagaaaatc ggcttcataa acaggttggt
     1021 tttgtaaacg aactctgtca gcttgtcgat gtagacgtcg aaatcagcaa tcggacgcgt
     1081 cgctacgccc gactccatcg ccgctttcgc caccgcagga gcgatcttaa cgatcaagcg
     1141 cggatcaaac ggttttggaa tgatgtattc cggaccaaag ctcagatcct gatcgccata
     1201 cgctgaagcc accacttcac tctgttccgc atgggccagt tctgcaatcg cgcgtaccgc
     1261 cgccagtttc atctcttcgt tgatggcggt tgcgccaacg tccagcgcgc cacggaagat
     1321 aaacgggaag caaaggacgt tgttcacctg gttcgggtag tcggaacggc cggtacagat
     1381 gatggcatcc ggacgcactt ctttcgccag cggcggcaga atttccggtt ccgggttcgc
     1441 cagcgccagg atcattggcg cacgggccat tttcttcacc atttcctggg tcagcacttt
     1501 cgggccggaa cagccgagga aaatatccgc gccttcaatc acatcatcga gggtacgttt
     1561 accgtcatcc accaccgcgt atgcggcttt ggtttccgcc atgtttggct cacggccctg
     1621 atagataacg ccttttgaat cgcaaaccac gatgttatgt ttttgcagac ccagcgctac
     1681 cagcaggttc atacaggcga ttgctgcggc acccgcgcca gaaaccacca tccgcacgtc
     1741 ggagatgttt ttctccacca cgcgcaagcc gttgaggatg gcggcagtgc tgataattgc
     1801 cgtgccgtgc tgatcgtcgt ggaataccgg aatattcatc cgctcgcgca gtttctgttc
     1861 aatatagaaa cattctggcg ctttaatgtc ttcgaggttg atgccaccga aggtcggttc
     1921 gagcgcagcg acgacttcaa taaatttgtc cgggtcgagt tcatcaactt caatgtcaaa
     1981 tacatcaatc ccggcgaatt tcttaaacag aacgcccttg ccttccatca ccggtttgcc
     2041 tgccagcgcg ccaatgttgc ctaaccccag caccgccgta ccgttagaga tcaccgctac
     2101 caggttaccg cgcgcggtat atttgtaggc ttttaacggg tctttttcga tttcaagaca
     2161 aggtgcggca acgcctggtg agtaggccag cgccagatcg cgctgcgttg ccagaggctt
     2221 ggttggagaa acctggattt tccctggaac tggaaattca tggaaatcaa gtgcactttg
     2281 ttttaactgg tcatccattt ggttgttcct ttcacgtaac gttcacaaat aaagtgtgtg
     2341 ggcaacagcc cctgtccaca acgtggcgca cattattacc ctgccggagt ctacagactt
     2401 tgagcaagtc caaactctca ccattaatat aatgttttgg taataatcct ataacactga
     2461 tgttacctgc ttaatccagc aataccatgc ctgtctgcta tgcttttttg atgcgtttag
     2521 cgaaatttct cagaagtgtg aattaacgca ctcatctaac actttacttt tcaaggagta
     2581 tttcctatga acgagttaga cggcatcaaa cagttcacca ctgtcgtggc agacagcggc
     2641 gatattgagt ccattcgcca ttatcatccc caggatgcca ccaccaatcc ttcgctgtta
     2701 ctcaaggctg ccggattatc acaatatgag catttaatag acgatgctat cgcctggggt
     2761 aaaaaaaatg gcaagaccca ggaacaacag gtggtcgcag cgtgtgacaa actggcggtc
     2821 aatttcggtg ctgaaattct caaaatcgta cccggtcgcg tgtcaacaga agttgatgca
     2881 cgcctctctt ttgataaaga aaagagtatt gagaaggcgc gccatctggt ggacttgtat
     2941 cagcaacaag gcgttgagaa atcgcgcatt ctgatcaagc tggcttcgac ctgggaagga
     3001 attcgcgcgg cagaagagct ggaaaaagaa ggtatcaact gcaacctgac gctgctgttt
     3061 tcttttgcac aggcgcgggc ctgtgcagaa gcaggcgttt ttctgatttc gccgtttgtc
     3121 gggcgtattt atgactggta tcaggcacgc aagccgatgg acccgtatgt ggtggaagaa
     3181 gatccaggcg ttaaatcagt acgcaatatc tacgactact ataagcaaca ccactatgaa
     3241 accattgtga tgggcgcgag cttccgtcgc accgaacaaa tcctcgcctt aaccggctgc
     3301 gaccgactga ctatcgcacc gaatttgctg aaggagctac aggaaaaagt ttcgccagtg
     3361 gtacgtaaat taatcccacc ttctcagacg ttcccacgcc cagctcccat gagcgaagcg
     3421 gaattccgtt gggagcacaa tcaggatgcg atggcggtag aaaaactgtc tgaaggcatt
     3481 cgtctgttcg ccgttgatca acgcaaactg gaagatcttc ttgccgccaa actataaacc
     3541 agccacggag tgttatatgt cccgaaaaga ccttgccaat gcgattcgcg cactcagtat
     3601 ggatgcggta caaaaagcca attctggtca tcccggcgcg ccgatgggca tggctgatat
     3661 tgccgaagtg ctgtggaacg attttcttaa acataacccc accgacccaa cctggtatga
     3721 ccgtgaccgc tttattcttt ccaacggtca cgcgtcgatg ctgctctaca gtttgctgca
     3781 tctgaccggt tacgacctgc cactggaaga actgaagaac ttccgtcagt tgcattcgaa
     3841 aacccctggt cacccggaaa tcggctatac gccaggtgtt gaaaccacca ccggtcctct
     3901 tggacaaggt ttggcgaacg ccgtcgggct ggcgatagca gagcgtacgc tggcggcgca
     3961 gtttaatcag ccggatcatg aaattgtcga tcacttcacc tatgtgttta tgggcgacgg
     4021 ctgcctgatg gaaggtatct ctcacgaagt ctgttcgctg gcgggcacgc tggggctggg
     4081 caagctgatt ggtttttacg atcacaacgg tatttctatt gatggggaaa ccgaaggctg
     4141 gtttaccgac gatacggcaa aacgatttga agcctatcac tggcatgtga tccatgaaat
     4201 cgacggtcac gatccacagg cggtgaagga agcgatcctt gaagcgcaaa gcgtgaaaga
     4261 taagccgtcg ctgattatct gccgtacggt gattggcttt ggttcgccga ataaagcagg
     4321 taaggaagag gcgcacggcg caccactggg ggaagaagaa gtagcgctgg cacggcaaaa
     4381 actgggctgg caccatccgc catttgagat ccctaaagag atttatcacg cctgggatgc
     4441 ccgcgaaaaa ggcgaaaaag cgcagcagag ctggaatgag aagtttgccg cctataaaaa
     4501 ggctcatccg caactggcag aagagtttac ccgtcggatg agcggcggtt tgccgaagga
     4561 ctgggagaaa acgactcaga aatatatcaa tgagttgcag gcgaatccgg cgaaaatcgc
     4621 tacccgtaag gcttcgcaaa atacgcttaa cgcttacggg ccgatgctgc ctgagttgct
     4681 cggcggttcg gcggatctgg ctcccagcaa cctgaccatc tggaaaggtt ctgtttcgct
     4741 gaaggaagat ccggcgggca actacattca ctacggggtg cgtgaatttg gtatgaccgc
     4801 tatcgccaac ggcatcgcgc accacggcgg ctttgtgccg tataccgcga cgttcctgat
     4861 gtttgttgaa tacgcccgta acgccgcgcg gatggcggca ctgatgaaag cgcggcagat
     4921 tatggtttat acccacgact caattggtct gggcgaagat ggtccgacgc accaggctgt
     4981 tgagcaactg gctagtctgc gcttaacgcc aaatttcagc acctggcgac cgtgcgatca
     5041 ggtggaagcg gcggttggct ggaagctggc ggttgagcgc cacaacggac cgacagcact
     5101 gatcctctca aggcagaatc tggcacaggt agaacgaaca ccggatcagg ttaaagagat
     5161 tgctcgtggc ggttatgtgc tgaaagacag cggcggtaag ccagatatta ttctgattgc
     5221 caccggttca gagatggaaa tcaccctgca agcggcagag aaattagcgg gagaaggtcg
     5281 caatgttcgc gtggtttccc tgccctcgac cgatattttc gatgcccagg atgaggaata
     5341 tcgcgagtcg gtactgcctt caaatgtttc tgcccgcgtg gcagtagaag cgggtattgc
     5401 cgattactgg tacaaatatg ttggtctgaa aggggcaatt gtcgggatga caggttatgg
     5461 ggaatctgct ccggcggata agctgttccc gttctttggc tttaccgccg agaatattgt
     5521 ggcaaaagcg cataaggtac tgggagtgaa aggtgcctga tggtgatcgc cggatgctga
     5581 ttgtcggatg tgacgctggt gcgtcttatc cggtctacat gtccccgcca ttttgtttaa
     5641 cgggtgatcc acaacgtggg ccaggcgtct ggcccatgcc agttatcgca ggtgggttct
     5701 gcggcgtaac gcaccaggcg aaaacgctga ccgtcaaagc gccagcgcgc ctgaatgcca
     5761 caatcgctta atccgcgccc ttttgctaag gtcaccagtt cacgcgattt ttcatcgaat
     5821 gttgcgttca tcagttccag ttcattcgtc tcctgaccgc tgttgaacgg caaacgtaac
     5881 cgaaccgggc gcgaggccag tggcttttta cgcgacacaa tccatgccaa atcaatggtg
     5941 ttataggccc ctgcctcaca gctaatcatc atcagcgctt tatcatcggt cagcgcagtg
     6001 acattcacct ctcgacgcaa tggatcaagc gagcagcgca gaccattcat ccgccagttt
     6061 ccataatcca gcaaatcgtt gcgttcttcg agtgagagtg gcgtcggcgt tgggttaacc
     6121 accgcgacct ctttcagcgc aggcgcgggc ggtacgctga gcggcggttc gtcccctttc
     6181 tttatccacg cggtttcact gccaacgcgc ttttgctgag catcaataaa caacaacgcc
     6241 gcttttaagc cactcagaga aatggtctga ttgccgtcgc gtaaggtgat cgccctccct
     6301 tcctgaatca tctgcaaaaa cgcggtgatg gttgccgtat catcggttac taataaccat
     6361 ggtgaaatcc gccacttgtc accacttaac gccaacggct cgccatctaa cagcagccgt
     6421 ggcgctatct ccccttctga cgcatccggc gacttcaatc cgccgcgctc aatacgtaaa
     6481 acggcatcgg tatgcgctcc ggcgctgcgg ctcagggtca tcaccagtcc attatgatca
     6541 cccgtattgc gcgccacaca aaaattttga ttattacagg tgacctgcca gtcggaaaac
     6601 gcccgttgcg ctggtgccgc ccacaccaaa gacgtcggca acaaagcgaa aaaaaagaga
     6661 aggaaaatgc gatagcgcat ggacggtacg cccccagaag ttcaaacaaa cagcacaagt
     6721 cgtatcttcg ttgcgcaggt agcgcggctc aatcggattt atcggattga gccaataata
     6781 aacaacgaaa tattattgtt atttttcagt ccattaaatg tgacatttgc aaatagttaa
     6841 gcaataacac cgtcttaccg tcacgtatct cgccggtttt aatcatctcc agcgcctggc
     6901 tgaacggcag ctcgagcact tcaatatctt catcttcgac accaccgccc gcgttagcgc
     6961 gctggctgtc actgtattcg gcgataaaga agtggattag ctcagtcaca ccgcctggcg
     7021 acatatacag ttcaaataat ttgcgcactt cgccaacttc ataacccgtc tcttcaatcg
     7081 cttctttacg aatacacact tccggttcgt cgttatccag cagcccggcg caggtttcaa
     7141 tcagttgccc gctttcattg ccattaaccc aggtagcgac gcggaactgg cgaatcagaa
     7201 ccacggtctt tttcttcgcg ttgtacagga ggatcgttgc accattaccg cgatcgtaaa
     7261 cttcacgttt atgacggata acttcgccat ctttgcgcgt gagatcgtaa gtaatgttgt
     7321 gcagggtgaa atagttatcg gagagaattt tgtctttaac gagggtgatt tgttgcgtca
     7381 taccgactcc acagcgcgaa atgaacaatt atcttacgct gtgaagtcgg gtttgtctgc
     7441 gcaggctatc agtgagattt gactgatttt acgcccagcc agtcaatgat cccctgtgcc
     7501 gcatgacgtc cttctgccat tgcggtaacc accagatccg caccacgcac ggcgtcacca
     7561 ccggcgaaga ttttcggatt cgtggtctgg taacggtact ggctttccac atccgcgatg
     7621 atgcggcccc atttgtctac cgttacaccg tgcgactcca gccacggcat cccgtgcgga
     7681 ttgaagccaa acgccataat caccgcatcg gctggcatga caaattcact gccttccacc
     7741 ggcactggac gccgacgtcc ctgggcgtcc ggctcgccaa gacgcgtgcg caggaaacga
     7801 atcccgcaga cgtgaccttg ttcattcagc tcaagcgcca ccggctggac gttaaattcg
     7861 aagttggccc cctcttcacg ggcgttcttc acctctttct tcgagcctgg catgttagct
     7921 tcatcacgac gataagcgca ggtgacgtta ctcgcgccgt ggcgcagcgc agtacgcaca
     7981 cagtccatcg cggtgtcgcc gccgcccagt accacgacgt taagtccggc agtattgata
     8041 aacggctctt ccggtagctc ttcgagtccc atcacctgtt tagtattggc gatgaggaac
     8101 ggcagcgcgt cataaacgcc cggcgcatct tcattgggta aacccgcttt catggaacgg
     8161 taagtgccta cgccaacgaa gaccgcgtcg tattgttcca aaagcgaatt caaagagaca
     8221 tctttaccca cttcacaatt gagttcgaag tgaatcccca tcgcgctgaa gatttcccga
     8281 cggcgtgcca gcagggattt atccagtttg aaagaaggaa tgccgaaagt gagcaagcca
     8341 ccgatttctg gatggcgatc gtacaccgtc accgccacgc cattgcgggt cagaacatcc
     8401 gcacaggcca gccctgccgg acccgcaccg ataatcgcca cccgcttgtc cactttggtg
     8461 acatgactta agtcaggacg ccaacctttc gccaacgcct gatctgaaat gtagcgttca
     8521 atgttgccga tagttactgc gccgtgctca tcgcgaatag tacaggcacc ttcacacaaa
     8581 cggtcttgcg gacaaacgcg tccggtaatt tccggcaggg tgttggtctg gtgagaaagc
     8641 tcgacggcgg cgttgatgtt tccggctttc accagttcaa tccactgtgg aatatggtta
     8701 tgcagcgggc aggtccattc acaaacgctg tgctcgccgc acttaaggca gcgcgaggct
     8761 tcccgttgtg cctgatcggc acagaatggc agataaattt catcaaaacc ggttttacgc
     8821 gcttcaatcg ccagtttgtc cggctcgcca cgcgcgggcg ttgcctgcat ttgctcgact
     8881 ttactcatta ccggcatttc ttgtgccgcg gtactggcat gccacggttg atgttcctga
     8941 cgtgcggtgc gcaagcggcg ggatttcgcc atgccggaga gtgcggcgtc agtgaccagt
     9001 tgcagcgcgt ctgccgggca attctcgaca caggcaggac cgttttcgcg ccctgcacaa
     9061 aggtcgcatt tatgcgccgt ggcttttact ttgcccgccg cgacgggtgt cagaacgatt
     9121 tgcatcgtac caaatggaca ggccaccacg caggatttac agccaataca cttttgctga
     9181 ttgacctgaa tgctgtcatc aacgtggctg attgcgccat tagggcagct acgggcgcag
     9241 ggcgcatctt cacaatggtg acaggtcact gcactacgtt gctgctgatg tttgataacc
     9301 gtaattcggg gatgaaaatg gcgttggctc aggacatgtt gctcgtcatt gtgagccatg
     9361 acacaggcga tttcacaagc atgacaaccc agacattgct gactgttggc cataataaaa
     9421 cgattcataa cgaccttctt ttttggttgt aaaaacctta ttctttatat gagtgttgtt
     9481 attacccgac ttacagggga atcggcaatg ttcatgtgcc cagaataagt aactatttcg
     9541 ctataaactg tggcagatca aataatccca tcactgacta aatttcgttt cagcgaactg
     9601 gaacattaat gattttttgt ggagaagacg cgtgtgattg ttaaacgacc cgtctcggcc
     9661 agtctggccc gggccttttt ttacattgtg ctgctgtcga ttctttccac gggtatcgct
     9721 ctgctaacgc tggcgagcag tttacgcgac gctgaggcta tcaatattgc cggatcgctg
     9781 cgtatgcaga gttaccgcct gggctacgac ttgcaaagtg gcagtccaca actcaatgca
     9841 catcgccagt tatttcagca ggcactgcat tcaccggtat taaccaatct caacgtctgg
     9901 tatgtgccag aggcagtaaa aacccgctat gcgcatctga atgccaactg gctggagatg
     9961 aataatcggc tcagcaaggg cgacttgccg tggtatcagg ccaatattaa taattatgtt
    10021 aatcagatag acctgttcgt gctggcttta cagcactacg ctgaacgcaa aatgctgctg
    10081 gtggtggcaa tttccctggc tggcggcatc ggtattttca cgctggtctt ttttactttg
    10141 cgccgcatac gccatcaggt ggttgccccg ctgaatcagc tggttaccgc cagtcagcgt
    10201 attgaacacg ggcagttcga ctcgccgccg ctggatacca gcctgccgaa tgagcttggc
    10261 ctgcttgcaa aaacctttaa ccagatgtcg agcgagctgc ataaattgta ccgttcgctg
    10321 gaggcgtcag tagaagaaaa gacccgcgat ctccacgagg ccaagcgtcg tctggaggtg
    10381 ttgtatcagt gttcgcaggc gctaaacacc agccagattg atgtgcattg tttccgccat
    10441 attttgcaga ttgttcgcga caatgaagcg gctgaatatc tggagttaaa tgtcggtgac
    10501 aactggcgga ttagcgaagg gcagccaaac ccggaattgc cgatgcagat tttaccggtg
    10561 acaatgcaag agacggttta cggcgaactg cactggcaaa atagtcacgt ttcatcatca
    10621 gaaccgctgc ttaacagcgt ttcgtcgatg ctgggacgcg gtttgtactt taatcaggcg
    10681 cagaagcatt ttcagcagtt attgttgatg gaagaacgtg cgaccatcgc ccgcgaattg
    10741 cacgactcgc tggctcaggt actttcttac ttacgtatcc agttgacgtt actgaagcgt
    10801 tcgataccgg aagataacgc caccgcacaa agtatcatgg ccgatttttc ccaggcgttg
    10861 aatgatgctt atcggcagtt acgcgagctg ttgactactt tccgcctgac gctgcagcag
    10921 gcggatctcc cctccgcgtt gagggaaatg ctggatacgt tacaaaatca aaccagcgcc
    10981 aaactgaccc tcgactgccg tctgccaacc ctggcgctgg atgcgcaaat gcaggtgcat
    11041 ttgttgcaaa ttattcgcga agcggtgctg aatgcgatga agcacgccaa cgccagcgaa
    11101 atcgccgtta gctgcgtcac cgcgccggat ggcaatcaca cagtctatat tcgcgacaac
    11161 gggattggca tcggtgaacc gaaagaaccc gaaggccatt atggtctgaa tatcatgcgc
    11221 gaacgcgcgg aaagattggg tgggacgctg actttttcac aaccttccgg cggcggcacg
    11281 ttagtgagta ttagctttcg ctctgcggag ggtgaggaaa gtcagctgat gtaatgcctc
    11341 ttattgacca aagaatagtg gcacttaagg ttcagtataa aagggcatga taatttacat
    11401 taactccttt ttttctccac gattggctcg taccttgccg ctacagtgaa gcaagccaag
    11461 cctacaacga tacgcagaaa cacgaggtcc tcttttaatg gcgaatttct ttattgatcg
    11521 ccccattttt gcctgggtgc tggcaatcct gttgtgtctg acaggtaccc tggcgatttt
    11581 ttcattgccc gttgaacaat accccgatct cgcgccaccg aatgtgcgag tgaccgctaa
    11641 ctatcccggc gcatcggccc agacgctgga aaacaccgtg acccaggtta tcgagcaaaa
    11701 tatgacgggc ctcgataatc tgatgtatat gtcatctcag agcagcggca ccggtcaggc
    11761 atctgtcacg ttaagtttta aagcaggcac cgatccggac gaagccgtgc agcaagtaca
    11821 aaaccagctg caatcagcca tgcgaaagtt accgcaggcg gtgcaaaatc agggcgtgac
    11881 ggtgcgtaaa accggcgata ccaacattct gaccattgcc ttcgtctcta ccgatggttc
    11941 gatggataag caggatattg ccgattatgt tgccagtaat attcaggacc cgttaagccg
    12001 cgtgaatggc gtcggggata tcgatgccta tggttcgcaa tattccatgc gtatctggct
    12061 ggacccggcg aaactcaaca gtttccagat gacggctaaa gatgtcaccg atgccattga
    12121 atcacagaac gcgcagattg cggttgggca acttggtggt acgccttccg tcgataagca
    12181 ggcgctcaac gccaccatta acgcccagtc actgctgcaa acaccagaac agttccgcga
    12241 tatcaccttg cgggtgaatc aggacggctc agaggtaagg ctgggcgatg tcgccaccgt
    12301 cgaaatgggg gcggagaaat acgattatct tagccgcttc aatggtaagc cagcctccgg
    12361 gctgggggtt aaactggcct ccggcgcgaa cgaaatggcg acagcggagc tggtgctcaa
    12421 tcgtctcgac gagctggcgc agtattttcc acacggactg gaatacaagg tggcgtatga
    12481 aaccacctcg tttgttaaag cctccattga agacgtggtg aaaacgcttc tggaagctat
    12541 cgccctggtt ttcctcgtta tgtatctgtt cctgcaaaac ttccgcgcca cgctgatacc
    12601 cactatcgcc gtgccggtgg tgttgatggg aaccttctcc gtgctttacg ccttcggtta
    12661 cagtgtcaac accttaacca tgttcgcgat ggtgctggcg atcggtctgc tggtggatga
    12721 tgccatcgtg gtggtggaaa acgtcgaacg tattatgagt gaggaaggac tcactcctcg
    12781 cgaagccaca cgtaaatcga tggggcagat ccagggggca ctggtcggga ttgcgatggt
    12841 gctttcggcg gtatttgtgc caatggcctt cttcggcggc accaccggtg ccatctatcg
    12901 ccagttctct attaccattg ttgcggcgat ggtgctgtca gtactggtag cgatgatcct
    12961 cactcctgct ctgtgtgcca cgctacttaa gccactgaaa aaaggtgagc atcacgggca
    13021 aaaaggcttt tttgcctggt ttaaccagat gtttaaccgc aactccgaac gctacgaaaa
    13081 aggggtggcg aaaattctcc accgtagcct gcgctggatt gtgatttatg tcctgctgct
    13141 tggcggcatg gtgttcctgt tcctgcgttt gccgacgtcg ttcttaccgc tggaagaccg
    13201 tggcatgttt actacctcgg tacagttgcc cagcggttca acccagcaac agaccctgaa
    13261 agtcgttgag caaatcgaga aatattactt cacctatgaa aaagacaaca tcatgtcggt
    13321 gtttgccacc gtgggttctg gccctggggg taacgggcaa aacgtggcgc gaatgtttat
    13381 ccgcctgaaa gactggagcg aacgcgacag taagaccggc acctcgtttg ccattatcga
    13441 gcgtgcaacg aaggcgttta acaagattaa agaagctcgc gttatcgcca gcagcccgcc
    13501 agcaattagc ggtcttggta gttctgcggg ttttgatatg gagttgcagg accacgctgg
    13561 agcgggtcac gatgcgctga tggcagcacg aaaccagttg ctggcgctgg cagcggaaaa
    13621 cccggagcta acccgtgtgc gccataacgg cctcgacgac agtccgcagt tgcagattga
    13681 tatcgaccag cgtaaagctc aggcgctggg cgttgctatc gacgatatta acgacacact
    13741 gcaaaccgcc tggggttcga gctatgtgaa tgactttatg gatcgcggtc gcgtgaagaa
    13801 agtctatgtg caggcagctg cgccgtatcg catgctgcca gatgacatca atctctggta
    13861 tgtccgaaat aaagatggcg gcatggtgcc cttctctgct ttcgcgacct cacgctggga
    13921 gacaggctcg ccgcgtctgg aacgctataa cggttattct gcggttgaga ttgttgggga
    13981 agccgcaccg ggggtcagta ccggtacggc gatggatatt atggaatcgt tagtgaagca
    14041 gttgccaaac ggctttggtc tggagtggac ggcgatgtcg tatcaggagc ggctttccgg
    14101 cgcgcaggct ccggcgctgt acgccatttc cttgctggtg gtattcctgt gtctggctgc
    14161 gctgtatgaa agctggtcgg tgccgttctc ggtaatgctg gtcgtgccgc tgggggttat
    14221 cggcgcgctg ctggcaacct ggatgcgcgg gctggaaaac gatgtttact tccaggtggg
    14281 cctgttaacg gtcattggtt tatcggcgaa aaacgccatc ctgatcgtcg aatttgctaa
    14341 cgagatgaac caaaaaggcc acgacctgtt tgaagcgacg ctccacgcct gccgtcagcg
    14401 tttacgcccg attctgatga cctcgctggc atttatcttc ggcgtattgc caatggcaac
    14461 cagcacaggt gccggttccg gtggtcagca tgcggtgggt actggcgtaa tgggcgggat
    14521 gatttcggcc actattctgg ctatttactt cgtgccgctg ttctttgtgc tggtgcgccg
    14581 ccgcttcccg ctgaagccgc gcccggaata agcaataaaa aaggcgacat gccaatgtgt
    14641 cgcccttttc aactttccga ttaagaacct gctcagcggg ttcttgctgt ttgtactttg
    14701 tctcaggaat tacttacgaa gcataacttc gataaagtct ttccagttcc ccagttcacg
    14761 ttcaatcata acaacctctc ttataattat gggtattcta cggaaacaat ataccgtggt
    14821 gaagctaatt tactcgattg ctgcgatgac tacctccggg ggacaaacct tatgtaaata
    14881 ctatggtcct gcggtgatga tttgtatgtg atacaccgca acatttcgag atattcatac
    14941 ggcatctaat actga
//
LOCUS       DADSZD010000107        14745 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.108, whole genome shotgun sequence.
ACCESSION   DADSZD010000107 DADSZD010000000
VERSION     DADSZD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14745
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.108"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(29..892)
                     /gene="potB"
                     /locus_tag="J1Z28_003025"
     CDS             complement(29..892)
                     /gene="potB"
                     /locus_tag="J1Z28_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBA7717839.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            complement(876..2012)
                     /gene="potA"
                     /locus_tag="J1Z28_003026"
     CDS             complement(876..2012)
                     /gene="potA"
                     /locus_tag="J1Z28_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBA7717840.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2262..3488
                     /gene="pepT"
                     /locus_tag="J1Z28_003027"
     CDS             2262..3488
                     /gene="pepT"
                     /locus_tag="J1Z28_003027"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBA7717841.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(3537..4658)
                     /locus_tag="J1Z28_003028"
     CDS             complement(3537..4658)
                     /locus_tag="J1Z28_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBA7717842.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4734..6194)
                     /gene="phoQ"
                     /locus_tag="J1Z28_003029"
     CDS             complement(4734..6194)
                     /gene="phoQ"
                     /locus_tag="J1Z28_003029"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBA7717843.1"
                     /translation="MKKLLHLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(6194..6865)
                     /gene="phoP"
                     /locus_tag="J1Z28_003030"
     CDS             complement(6194..6865)
                     /gene="phoP"
                     /locus_tag="J1Z28_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBA7717844.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(7035..8405)
                     /gene="purB"
                     /locus_tag="J1Z28_003031"
     CDS             complement(7035..8405)
                     /gene="purB"
                     /locus_tag="J1Z28_003031"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBA7717845.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(8409..9050)
                     /gene="hflD"
                     /locus_tag="J1Z28_003032"
     CDS             complement(8409..9050)
                     /gene="hflD"
                     /locus_tag="J1Z28_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBA7717846.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPCSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(9086..10192)
                     /gene="mnmA"
                     /locus_tag="J1Z28_003033"
     CDS             complement(9086..10192)
                     /gene="mnmA"
                     /locus_tag="J1Z28_003033"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBA7717847.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(10246..10707)
                     /gene="nudJ"
                     /locus_tag="J1Z28_003034"
     CDS             complement(10246..10707)
                     /gene="nudJ"
                     /locus_tag="J1Z28_003034"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBA7717848.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(10717..11370)
                     /gene="rluE"
                     /locus_tag="J1Z28_003035"
     CDS             complement(10717..11370)
                     /gene="rluE"
                     /locus_tag="J1Z28_003035"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBA7717849.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWRDATD"
     gene            11542..12792
                     /gene="icd"
                     /locus_tag="J1Z28_003036"
     CDS             11542..12792
                     /gene="icd"
                     /locus_tag="J1Z28_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBA7717850.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(12906..14048)
                     /locus_tag="J1Z28_003037"
     CDS             complement(12906..14048)
                     /locus_tag="J1Z28_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7717851.1"
                     /translation="MAARPRSHKISIPNLYCKLDKRTGKVYWQYKHPLSGRFHSLGTD
                     ENEAKQVATEANTIIAEQRTRQILSVNERLERMKGRRSDITVTEWLDKYISIQEDRLQ
                     HNELRPNSYRQKGKPIRLFREHCGMQHLKDITALDIAEIIDAVKAEGHNRMAQVVRMV
                     LIDVFKEAQHAGHVPPGFNPAQATKQPRNRVNRQRLSLPEWQAIFDSVSRRQPYLKCG
                     MLLALVTGQRLGDICNLKFSDIWDDMLHITQEKTGSKLAIPLNLKCDALNITLREVIS
                     QCRDAVVSKYLVHYHHTTSQANRGDQVSANTLTTAFKKAREKCGIKWEQGTAPTFHEQ
                     RSLSERLYREQGLDTQKLLGHKSRKMTDRYNDDRGKDWIIVDIKTA"
     gene            complement(14038..14274)
                     /locus_tag="J1Z28_003038"
     CDS             complement(14038..14274)
                     /locus_tag="J1Z28_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBA7717852.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            complement(14414..14653)
                     /locus_tag="J1Z28_003039"
     CDS             complement(14414..14653)
                     /locus_tag="J1Z28_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBA7717853.1"
                     /translation="MFRVIDPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRRK"
BASE COUNT         3608 a         3682 c         3709 g         3746 t
ORIGIN      
        1 ttgccattaa ctctttgaat actcagcact aatcatctat ttcggttgcc ttcttattca
       61 gcagacgaga agcgcgccag taaaccagca acatcaggcc cattacgata gtcagcgtaa
      121 tgctggtggc tgcaccaaac ggccagtcac gaatattgag gaactgtacc ttgatgacgt
      181 taccgatcag caggtttttc gcaccgccca tcaggtcgga cacatagaac aggcccatcg
      241 ctggcagcat caccagcaga catccggcaa taattcccgg catcgttagt gggataatga
      301 tgcggataaa ggtctgcaac ttgctggcac cgagatcgcg cgccgcctca agcaacggct
      361 tatccagttt ttcgatactg gagtacagcg gcatcaccat aaacggcagc agaatgtaaa
      421 ccagaccgat aatcaccgca ctaggcgtga acatgatgcg aattggtgta tcgataacgc
      481 ccagccagag caaaaactcg ttgagatagc ctttggtgct gaggaaaatt ttcagcccgt
      541 aaatacgaat caatgagttg gtccagaacg gaacaatcag cagaaacagc agcagcggac
      601 gcaccttgtg tggcagcttc gccagaaacc aggcaaaagg atagcccagc accaggcagg
      661 cgagggtggc gatcagcgcc atattcagcg agtgcaatag cacttcaaaa tagagcggat
      721 cgagcagacg cgtgtagtta tccagcgtaa agaccatttt gacgaaactg gcgtcgtcgc
      781 gggtcaaaaa gctggtgcca atgatcatca ggttgggcag aaagacaaac aacacaagcc
      841 aaccgacaat agtgacaatc actacattct ggaacttact tgtgttcttc atcagccagt
      901 acgacctccc agctttctac ccaattaatg gccatttttt ggtcgagaga gtggtcaaag
      961 tcaggatcgt cttcattgaa gaattcgctg accatcacca tcttgccatt ttccagctca
     1021 acaaccgact ccagcgtcat gcctttgtag ttacgctcgc gaacgtaacc aatcagccct
     1081 tcggcgtggt tgtcgtcgtt aatctcttca acacgtaagt cttccgggcg cagcagaaca
     1141 tgcagttttt gccccggttc aacggcgaag ttaacgtaga tattacattc gcggccttca
     1201 acgttggcgc gtacgcgctg ctcgtcgaga cgttcgatga cagtggcgtt aaacatattg
     1261 atttcgccaa taaacccggc aacaaacagg tttttcggct cttcgtagat ttcacgcggc
     1321 gtgccgtctt gctcaatgcg accatcgcgc atcaccacga tcctgtccga catggtaagt
     1381 gcttcttcct ggtcgtgagt tacgaacacg aatgtaatgc caagcttacg ctgtaacgct
     1441 ttcagctcgt tctgcatttg cttgcgcagt ttgtaatcca gcgctgagag cgactcatcc
     1501 agcaacaaca gacgaggctt gttaaccacc gcgcgagcaa tggcgacgcg ttgctgttga
     1561 ccaccagaga gctgatgcgg tttgcgttga gcgaaggttt ccagctgcac catccgcagg
     1621 gcttccatca cgcggggcgt aatttcagca gcgggggttt tttgcatgcg caacccaaag
     1681 gccacatttt cgaacacggt catgtggggg aaaagtgcgt agctttggaa aacagtgttc
     1741 acatagcggt tttccgccgg aacgtgggtg atgtcctcgt tatccagcat gatgcgtccg
     1801 gaatcaacag tttccagacc tgcaatcagg cgaagaacgg ttgttttacc gcagccagaa
     1861 gggccaagca gcgtgaggaa ctcgccattg ttgatagtca gatccagctg gggaatgacc
     1921 tctttaccat caaagcattt gcgaattccc gccaattgca ccagcggtga aagcgaactc
     1981 ggttgtttat tcaatttttt actctgtccc atgtaaacgc aacggatggc ttaccgatgc
     2041 ggggtttgtg gttaaccacc ttggtgactc ttaatgaggg cggtaattct acggcaaacc
     2101 gcttgaatcg ccaatctttg ttgtgaatta ctggcttagc tttatattca ttaaggtaat
     2161 gctgataaat attcccgctt gcagggctaa aagtgacctg acgcaatatt tgtcttttct
     2221 tgcttcttaa taatgttgtc acaaaaagtg agggtgacta catggataaa ctacttgagc
     2281 gatttttgaa ctacgtgtct ctggataccc aatcaaaggc aggggtgaga caggttccca
     2341 gcacggaagg ccaatggaag ttattgcatc tgctgaaaga gcagctcgaa gagatggggc
     2401 ttatcaatgt gaccttaagt gagaagggca ctttgatggc gacgttaccg gctaacgtcc
     2461 ctggcgatat cccggcgatt ggctttattt ctcatgtgga tacctcaccg gattgcagcg
     2521 gcaaaaatgt gaatccgcaa attgttgaaa actatcgcgg tggcgatatt gcgctgggta
     2581 tcggcgatga agttttatca ccggttatgt tcccggtgct gcatcagcta ctgggtcaga
     2641 cgctgattac caccgatggt aaaaccttgt taggtgccga tgacaaagca ggtattgcag
     2701 aaatcatgac cgcgctggcg gtattgcaac aaaaaaacat tccgcatggt gatattcgcg
     2761 tcgcctttac cccggatgaa gaagtgggca aaggggcgaa gcattttgat gttgacgcct
     2821 tcgatgcccg ctgggcatac accgttgatg gtggtggcgt cggcgaactg gagtttgaaa
     2881 acttcaacgc cgcgtcggtc aatatcaaaa ttgtcggtaa caatgttcac ccgggcacgg
     2941 cgaaaggagt gatggtaaat gcgctgtcgc tggcggcacg tattcatgcg gaagttccgg
     3001 cggatgaaag cccggaaatg acagaaggct atgaaggttt ctatcacctg gcgagcatga
     3061 aaggcaccgt tgaacgggcc gatatgcact acatcatccg tgatttcgac cgtaaacagt
     3121 ttgaagcgcg taaacgtaaa atgatggaga tcgccaaaaa agtgggcaaa gggttacatc
     3181 ctgattgcta cattgaactg gtgattgaag acagttacta caatatgcgc gagaaagtgg
     3241 ttgagcatcc gcatattctc gatatcgccc agcaggcgat gcgtgactgc gatattgaac
     3301 cggaactgaa accgatccgc ggcggtaccg acggcgcgca gttgtcgttt atgggattac
     3361 cgtgcccgaa cctgttcact ggcggttaca actatcatgg taagcatgag tttgtgactc
     3421 tggaaggtat ggaaaaagcg gtgcaggtga tcgtccgtat tgccgagttg acggcgcaac
     3481 ggaagtaagc gaaaagggat gcggcatgtg atgccgcatc cggcttaaat ccaaacttac
     3541 ccttcgaaga accagtatcc gctattgacc agcgccgcga gcatcgcgag gaatgacgga
     3601 tcttccagcg catcgccaaa attctccgcg gtcagcgcaa tgttgctggc gagcgcatcc
     3661 agtgccggac ggtgcgggga atcgatcttc tcaccattgg catacacgtc gtcgccaatg
     3721 cgcaatacgc gcagaccccc caggcgcacc agcacatcac cttgtttcag cgcatcgtag
     3781 atttcatccg gctgataagg cggctctggc ggcgcgatat ccagttcatg acgtgactgg
     3841 gatataaact cgccaaacca ttgtttaaag tgttccggct ggttgatcaa ttcgagcatc
     3901 atctcacgca gtttatccat ctcttgcggc agaacgtccg caggatgagc gcgaggtgga
     3961 acatcaggat cgctgtagta gttgccgccc agttcacgtt gcagcacata atcggcaaat
     4021 ccgctaatca gttcccgcgt atttggcgcg cgaaaaccca cggaatagtt catcgcattt
     4081 tccagcgcgt agccttcatg cgggaatcct ggcggaatat aaagaatatc gccaggctcc
     4141 agctcttcat cgatgatggc ttcgaacgga tcgacctgta acaggtccgg gtgcgggcag
     4201 tgctgtttca tttgcagctt ttcgcccact cgccagcgac gacgaccggt accctgaatg
     4261 ataaacacgt cgtactgatc gagatgcggg ccgacgccgc cgcccggtac agaaaaagag
     4321 atcatcagat catcaatacg ccagtccggt agttcacgga acggtcgcat tagcgcggcg
     4381 gtcggctcat gccagtggtt cactgcttgc actaacaatg accagttagt ttcaccgaga
     4441 tgatcgtagc tttcgaatgg accgtggctg acctgccatt tgccatcctg atgactgacc
     4501 agtcgactgt cgacttcgct ttccatcgcc agacccgcca actcgtctgg agagattggg
     4561 tcaataaaat tattaaagcc gcgttttaac accaccgggc gtttctgcca gtgacgttca
     4621 agaaaatcgg gccagttaag agtgagttgg tattccatgt taagcatcca caggctggta
     4681 tctgcaaccg attataacgg atgcttaacg taatgcgtga agtatggaca tatttattca
     4741 tctttcggcg cagaatgctg gcgaccaaaa atcacttcca tccgcgcacc gcccagcatg
     4801 ctctctccgg cgacgatttt accctcatat tgctcggtga tttcgcgggc taccgccagc
     4861 cccacacctt gcccagggcg taaagtatca acccgttgac cacggtcgaa aatgacctct
     4921 cgcttgctta atggaatacc ggggccatca tcctcgacca caatatagag atgctcgtcg
     4981 gtttgccttg cagaaatttc gacaaattcg aggcaatatt tacaggcatt atccagcacg
     5041 ttgcccatca cttcgacaaa atcattctgc tcaccaacaa agctgatttc tggcgagata
     5101 tcgagagaga tattgacccc tttgcgttga tacactttgt tcagcgccga ggtgagattg
     5161 tccaacagtg gcgcgaccgg atgcagctcg cggctaagca atgtcccgcc gcgcatactg
     5221 gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc cagcattacc
     5281 ggctcagcat cactgacgct catcttctca ctacgcagag aacgcagcgt actttgcagc
     5341 accgccagtg gcgttttcag actatgggtc aggtcggtga gcgttgtacg atatttgtcg
     5401 taacgttcgc gttcactttt taacagtcgg ttcaggtttc gtaccagact ggtcagttct
     5461 cgcgttgtgg ctggattgag caattcgcgg ttatgttctt ccagttcgcg gacttctttt
     5521 gccagggctt cgatggggcg taaactccac caggcggcga cccacagcag cgggatcact
     5581 aacagcagat tggctgagag cacatagata aaccagctcc agaccatata ggaacttttt
     5641 agctccaccg gaatggtatc caccaccaca atggttaact ttggcatccg cgatgttgcc
     5701 gggtagacgt ttaccgccac cgagtgggtc atctccgcgt cgtcatcatc ttcccgcact
     5761 tcctgcaact gttgctgtat cgaatgatct ccactcagca agaggctggt atcgttaaca
     5821 tccgcttcaa tttcatgaaa accattcgat ttcagccagt caggctggat catcttcatc
     5881 agccagggca cgtcacgttg cgcccataaa agctgcccgt tctcatcata aattagtgtc
     5941 atcgtggggc tttgcttgtc gatattttcg ggtaactcga catgcaactt attgttttcc
     6001 cacttcgcaa gggtatagaa cagattgctc tcgccacgta acagccgaaa cgtggtttta
     6061 tcgaaactga cgctataacc gatcagcgca accattccgt aggcaagcga aagcaccaat
     6121 actactgctg ccgttgctaa cagaaaacgt acccgcagcg agagcgggaa aaaaagatgc
     6181 agtaattttt tcatcagcgc aattcgaaca gatagccctg gccgcgaacg gtggtaatca
     6241 cttcttgcgg atattgtgcc tgtatttttt tgcgcagacg tcccatcagt acatctatgg
     6301 tatgactttc ccgcagctcc gcatccgggt agagttgtag cattaacgaa tctttgctga
     6361 ccactttgcc attattgcgt atcaacgttt ccatgatggt gtattcgaac gcggtcagtt
     6421 tgatcacttc gtcattaata gataattcac ggcgggagag atcaacctga aacggtggta
     6481 gcgaaatgac ctgtgaagcc agaccgctat tacgccgcat taatgcctgc attcgcgcca
     6541 tcacctcttc aatatgaaac ggtttagtca cgtaatcatc agcaccggca cttaatactt
     6601 cgactttgtc ctgccagctt tcacgggcgg ttaataccag aatcggcagt gaaacatcat
     6661 tgctacgcca gcggcgaatc agtgacagac cgtcctcgtc aggcaaaccg agatcgacaa
     6721 tcgcaatatc cggtaaatgt tcattgagat aataatcggc ttctttggca tcttctgcat
     6781 catcgacctg atgaccagca tcctgaatct gaactttaag gtggtgacgt aacaacgcat
     6841 tgtcttcaac aaccagtacg cgcattttta tttctccctg tcttaattat taaaatagtg
     6901 taacgcgatt atgtggttat gggggtaaac attaaataaa ccagcggggg aggggaggta
     6961 aagtgaaaaa ataaaaagcg gataatctta ataagcaggc cggacagcat cgccatccgg
     7021 cactgttttg aggcttattt cagctcatca accatggtga tggcgcgacc aatgtagtta
     7081 gccggcgtca tcgctttcag gcgggctttc tcttcttctg gcaacgccag accatcgata
     7141 aactgcttca tgccttcggc gtcaacgcgc ttaccgcgag tcagctcttt cagcttctcg
     7201 tacggttttt cgatgccata gcgacgcata actgtctgga ttggttcagc cagcacttcc
     7261 cagttgtgat ccagttcatc cagtagatgg tcacggttca cttccagttt actcacgcct
     7321 ttcagggtgg attgatacgc aatcagcgca taaccgatac ccacgccgag gttacgcagc
     7381 acggtggagt cggtcaggtc acgctgccag cgggaaactg gcagtttgct tgccaggtgc
     7441 tgcaataccg cgttggaaag ccccaggttt ccttcggagt tttcgaagtc gatcgggtta
     7501 actttatgcg gcatggtgga agaaccaatc tcaccagcaa tggttttctg tttgaagtgg
     7561 ttaagggcga tataacccca gacgtcacgg tcaaagtcga tcagaatggt gttgaagcgc
     7621 gcaacgcaat caaacagttc ggcaatgtag tcgtgcggtt cgatctgggt ggtgtacggg
     7681 ttccactgaa tacccagcga ggtgacgaac tcttcgctga actgatgcca gtcaacttcc
     7741 gggtaagctg cgatgtgggc gttgtagtta ccgaccgcac cattgatttt gccgaggatc
     7801 tccacctgat taagctggcg gtactggcgc tccatacggt aggcgacgtt tgccatctct
     7861 ttaccgatgg ttgacggcgt ggctggctga ccgtgggtac gggacagcag cgggatatcg
     7921 cgatactgaa cggcgagatc tttaatgcca tcaatcagtt gacgccagta cggcaggatc
     7981 acttcatcac gcgcggtttt cagcattaat gcgtgggaga ggttattgat atcttccgaa
     8041 gtacaggcaa agtggatgaa ttcagaaacc gcgtgcagtt ccgggatctc cgccactttt
     8101 tctttcagga aatactcaac cgctttaacg tcgtggttag tggtacgctc gatggttttg
     8161 atgcgtgctg catcttcttc gctgaaactg gcgacaattg catcaaggta accgattgcg
     8221 tcggcagcaa aagcaggaac ttccttgatc gctgcgtgcg cggccagttt ttgcagccaa
     8281 cgtacttcaa cttgtacacg gaatttcagc aaaccgtatt cgctgaaaat cccgcgcagc
     8341 gcgctgactt tatcgccgta gcgtccatcg acaggggaaa cggcggtcag tgaggataat
     8401 tccatagatc acaactccgg ggttaaatga gcaagaattt gttttgcctg agtggtcagg
     8461 cgattacgag aaaacatcag ttgcagacga ccgccgccga cctggtgcca gagcacggcg
     8521 gcgcgaatgc ctgccagcag ggttgcgcga actttcgcct gcacctgtgg gctttgcagt
     8581 acagcagggg aaccagtgac ctgaatgcgc gggccaagcg ggctgataac atcaacatag
     8641 atggcagcca tcgcgctcat cagcgtttcg gactgtaaat cgaagtgttc gagctggcgt
     8701 tgcaggccgt tgatccggtt gcccagagtg tcgagcgcac ctttcgctga ggagagtttg
     8761 cgctcaagca ccatcagact gagtgtgtag cgggttaatt cggcgtttaa gccctggcga
     8821 ctgctggcat tgagtacgcc gagcaaggtt tccagcccga cgcgcaggtt ggcttcgcta
     8881 ccgccaaaaa ccgccagcgt tgagcagggg ttcatatcga taatactgtt gagtgagacg
     8941 tgtagcgcat cgccatcaca atgcccctgg tgagcgagtt gttgcaccag gcgtgccgac
     9001 tgacaaatac cggcaagggc gagggtgatg tcatagtaat tctttgccac gttcactgct
     9061 tccttgtttt aagtaaagat aataatcaga ccggcagcgg cagacgctgc tcaataatac
     9121 cgccaccgag gcacacttca ccgttataga aaacggcaga ctggcccggc gtcacggcgg
     9181 caaccggctc atcgaaaatc acttcaatgc gatcagcgtc cagcgccttg acggtgcacg
     9241 ggatgtcggt ctggcgatag cgggttttta ctgtgcaacg catagtaccg gtgaacggct
     9301 cgcgatcgac ccagtgcagc tgctgggcaa tcagaccgac agacatcaga cgcgggtgtt
     9361 catggccttg cgcaacaatc agaatgttgt tttcgacgtc tttatcgacc acataccacg
     9421 gttcttcggt gccgtctttg gtgccaccga tacccagacc tttacgctga ccgagggtgt
     9481 gatacatcag cccctggtgc tcaccaattt catcgccatc gacggtaatg attttgcccg
     9541 gttgcgccgg gagataacgg cccaggaact cgcggaattt acgctcgccg ataaagcaga
     9601 taccggtaga gtcttttttc ttcgcggtga ccagaccgag atcttcagca atcttacgca
     9661 cctgcggttt ttccagttcg ccgaccggga acaggctttg cgcaatttgc tcatggctga
     9721 gcgtataaag gaagtagctc tggtctttat tgctgtccag gccacgcagc aggcggctct
     9781 tgccatcgac atctgcccga cgtacgtaat gaccggtagc gatataatcg gcacctaaat
     9841 cttcggcggc aaattcgagg aaagctttaa atttgatctc tttgttgcac agaatatccg
     9901 gatttggcgt gcgaccggct ttatattcgg caaggaacag ttcgaagacg ttgtcccagt
     9961 actcggcagc aaagttaacg gtgtgcagtt caatgccgag cttgtcgcag acagcctggg
    10021 catcagccag atccgctgcc gctgtgcaat attcctcacc gtcgtcttct tcccagttct
    10081 tcataaacag gccttcgacc tgatatccct gttgttgcaa cagccaggca gaaacggagg
    10141 aatcgacacc gccggacatg ccgacgatta ctttttttgc ggtttcagac attggatcac
    10201 tcacgacatt gaacttcaag gcggcgtatt ctatcacgca gccttttaga tgacaccctt
    10261 tgtaaaaggc cagttaaaat cgccaatcat ctccagcgga tagcgttgcc cgctttgata
    10321 acaacgaata ctttccgcca ccagcggcga acgaagattt gacgcctgta aaatttcttc
    10381 ggcgctgacc caacggcagc agtcgatatc gctgtcatga ggctgcgtcg ggcatatttg
    10441 ctcaagctca atggcaaaga ggaaacgcaa aaacggcgtt ttatccggcg caatccactg
    10501 atgcatacga ataaagtgtt gcggctgcgc gctaatgccg gtttcttccc acagctcacg
    10561 ggcggcggct tccactaagg tttcatcggc ttccaggtgc ccggcaggtt ggttccataa
    10621 cgctttacca ttaatcgtct cttcaacgac taaaaatttg ccttctgcgt gcaccacgca
    10681 ggcaacggta acgtgcggtt taaacatttt gcatccttaa tctgtcgcgt ctcgccattc
    10741 gccattggca agattatcca aagagtaatc ccccatcgca tagcgaatca gtcgcagcgt
    10801 ggggaaacca acatgggcgg tcatgcggcg cacctggcga ttacgtccct catataaggt
    10861 gatcttcagc cagctggtgg gaatactttt gcgctcacga attggtggat tccgcggcca
    10921 taaccacgcg ggttcttcaa ccagctccgc gcctgcgggc agggtagggc catcatttaa
    10981 ggttacgcca ttgcgcaagg cttcaagtgc gtcttgtgtg ggaatacctt ccacctgcac
    11041 ataatagatt tttccggtgc gtttacccgg ctgggttaaa cgcgcctgca acgcgccgtt
    11101 attggtcagc accagtaacc cttcgctgtc gcggtcgaga cgacctgccg catacacccc
    11161 ctgaaccggg atgaattctt ttaatgtttt gcgtccggct tcatcggtga actgcggaag
    11221 aacatcgtag ggtttattga acaggatcac acgcgtgggc tggttttcag gtttacgcct
    11281 ggtagaacgt tgcgagctga atcgcttaac ctggtgattt ctaaaagaag ttttttgcat
    11341 ggtattttca gagattatga attgccgcat tatagcctaa taacgcgcat ctttcatgac
    11401 ggcaaacaat agggtagtat tgacaagcca attacaaatc attaacaaaa aattgctcta
    11461 aagcatccgt atcgcaggac gcaaacgcat atgcaacgtg gtggtagacg agcaaaccag
    11521 tagcgctcga aggagaggtg aatggaaagt aaagtagttg ttccggcaca aggcaagaag
    11581 atcaccctgc aaaacggcaa actcaacgtt cctgaaaatc cgattatccc ttacattgaa
    11641 ggtgatggaa tcggtgtaga tgtaacccca gccatgctga aagtggtcga cgctgcagtc
    11701 gagaaagcct ataaaggcga gcgtaaaatc tcctggatgg aaatttacac cggtgaaaaa
    11761 tccacacagg tttatggtca ggacgtctgg ttgcctgctg aaacccttga tctgattcgt
    11821 gaatatcgcg ttgccattaa aggcccgctg accactccgg ttggtggcgg tattcgctct
    11881 ctgaacgttg ccctgcgcca ggaactggat ctctacatct gcctgcgtcc ggtacgttac
    11941 tatcagggca ctccaagccc ggttaaacac cctgaactga ccgatatggt tatcttccgt
    12001 gaaaactcgg aagacattta tgcgggtatc gaatggaaag cagactctgc cgacgctgag
    12061 aaagtgatta aattcctgcg tgaagagatg ggcgtgaaga aaattcgctt cccggaacat
    12121 tgcggtatcg gtattaagcc gtgttcggaa gaaggcacca aacgtctggt tcgtgcagcg
    12181 atcgaatacg caattgctaa cgatcgtgac tctgtgaccc tggtgcataa aggcaacatc
    12241 atgaagttca ccgaaggcgc gtttaaagac tggggctacc aactggcgcg tgaagaattt
    12301 ggcggtgaac tgatcgacgg cggcccgtgg ctgaaagtta aaaacccgaa taccggcaaa
    12361 gagatcgtca ttaaagacgt gattgctgat gcattcctgc aacagatcct gctgcgtccg
    12421 gctgaatatg atgttatcgc ctgtatgaac ctgaacggtg actacatttc tgacgccctg
    12481 gcagcgcagg ttggtggtat cggtatcgcc cctggcgcaa acatcggtga cgaatgcgcc
    12541 ctgtttgaag ccacccacgg tactgcgccg aaatatgccg gacaggacaa agtaaaccca
    12601 ggctctatta ttctctccgc tgagatgatg ctgcgccata tgggctggac tgaagccgca
    12661 gacctgattg ttaaaggtat ggaaggcgca atcaacgcca agaccgtaac ctatgacttc
    12721 gaacgtctga tggaaggcgc taaactgctg aaatgttcag agtttggtga cgcgatcatc
    12781 aaaaatatgt aattaccaca tgtgttaaat attataacgg gcgtataaca cgcccgttgt
    12841 tttatgatga tgtaaaatct tccccaaaac tttccccaaa acccttcccc aaaactggct
    12901 attttctatg ctgttttgat atctacgata atccagtctt taccacgatc atcattgtat
    12961 cggtcggtca tttttctgga tttatgacct aacaactttt gcgtatccag accctgttcc
    13021 cgatataacc gttctgacag agatcgctgc tcatgaaatg tgggcgcagt tccttgctcc
    13081 cattttatgc cacatttttc cctggccttt ttaaaagccg ttgtcagagt atttgcagaa
    13141 acctggtctc ctctgtttgc ttgagaggta gtgtgatggt aatggaccag atatttacta
    13201 acaacagcat ccctgcactg agatataact tcacgaaggg taatattcag agcatcgcat
    13261 ttcaggttaa gcggaatagc aagttttgaa ccggtttttt cctgagtaat gtgcaacatg
    13321 tcgtcccaga tatcagagaa tttcaaattg cagatatcgc ctaaacgttg tccagtgaca
    13381 agagcaagta gcatcccgca ttttaaatag ggctgccgtc tgcttacgct gtcaaatatt
    13441 gcctgccatt cgggcagtga taatctttgg cggtttactc gatttcgcgg ttgttttgtt
    13501 gcctgcgctg ggttaaatcc tggcggaaca tgtcctgcgt gttgtgcttc tttgaagacg
    13561 tcgatcaaca ccattctcac gacttgcgcc atcctgttat gaccttcagc ctttacagca
    13621 tcaattattt cggcaatatc aagtgcggta atatccttga ggtgttgcat tccacaatgc
    13681 tcacggaaaa gacgaatggg tttgcctttt tgccgatagg agttgggtct tagttcatta
    13741 tgttgcagcc tgtcctcctg gatagaaata tatttatcaa gccattctgt caccgtaatg
    13801 tctgagcgcc tgcctttcat tctttccaga cgctcattga cgcttaatat ttgtcgagta
    13861 cgttgttcag caataatggt atttgcttca gtagcaactt gttttgcttc attctcatca
    13921 gttcctaagc tatgaaaacg accggatagt ggatgtttgt attgccaata tacctttccg
    13981 gttcgcttat ctaatttgca atataaattg ggtatagaga ttttgtgaga tcggggtcta
    14041 gcagccatca gcgattatcc tttggagttt tgggtttgcg tttattggga gttgcggttc
    14101 tgcaagcgtt cctacaaaac gggaatttcg gtcaatcatc cagtagcgac caacttttat
    14161 agcgggtggg gccatcattt tcccttgcgc gtattttttc agaactcgct cacttggtgc
    14221 taagtcccca aattcttctt tagcccagtc ctgtaaagtg attagtcgag acatttgtcc
    14281 tcctcttagc tgctgaggga gtttgtgacc gatatatctg acatgatatt aagctcatgg
    14341 caggtacatc tcttgactgg tcatagagat aaatttaatg ctgagaaatg cagtattgaa
    14401 tttatcaatt tttctatttc ctgcgcatgt cacgtaactt cttaatgtgt tctgccgttt
    14461 cgatctcttc tgctatccga tctgcatcag ctttattcac aggttcaaag tcatgattaa
    14521 agcggaacat gctggcgata catgttctgc cttttcggat gtagtgaact ttgttgtggg
    14581 tagaacgcag gattttgcag ggagtgccgt ggtggtcgac gtaccaggtg ttagggtcaa
    14641 tcactctgaa cattcttgac cttgtgttgt gcggattgat attcagtgct gatattcacc
    14701 tttatagcga acacctttac cggtttatcg ccaaagtgcg gatgt
//
LOCUS       DADSZD010000108        14597 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.109, whole genome shotgun sequence.
ACCESSION   DADSZD010000108 DADSZD010000000
VERSION     DADSZD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.109"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(12..1466)
                     /gene="xylB"
                     /locus_tag="J1Z28_003040"
     CDS             complement(12..1466)
                     /gene="xylB"
                     /locus_tag="J1Z28_003040"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBA7717854.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1538..2860)
                     /gene="xylA"
                     /locus_tag="J1Z28_003041"
     CDS             complement(1538..2860)
                     /gene="xylA"
                     /locus_tag="J1Z28_003041"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBA7717855.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3226..4218
                     /gene="xylF"
                     /locus_tag="J1Z28_003042"
     CDS             3226..4218
                     /gene="xylF"
                     /locus_tag="J1Z28_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7717856.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4296..5837
                     /gene="xylG"
                     /locus_tag="J1Z28_003043"
     CDS             4296..5837
                     /gene="xylG"
                     /locus_tag="J1Z28_003043"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBA7717857.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALKKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5815..6996
                     /gene="xylH"
                     /locus_tag="J1Z28_003044"
     CDS             5815..6996
                     /gene="xylH"
                     /locus_tag="J1Z28_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBA7717858.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7074..8252
                     /locus_tag="J1Z28_003045"
     CDS             7074..8252
                     /locus_tag="J1Z28_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR family transcriptional regulator"
                     /protein_id="HBA7717859.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8360..9184)
                     /locus_tag="J1Z28_003046"
     CDS             complement(8360..9184)
                     /locus_tag="J1Z28_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBA7717860.1"
                     /translation="MILTPIRRYGAMMLMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9504..11534
                     /gene="malS"
                     /locus_tag="J1Z28_003047"
     CDS             9504..11534
                     /gene="malS"
                     /locus_tag="J1Z28_003047"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBA7717861.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLMLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQLDTRSGTPT
                     LMISIQNAVEPVASLVRECPKWDGLPLTLDVSATFTEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPNNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGSKADLRTLVDSAHQRGIRIIFDVVMNHTGYATLADMQEYQFGALYL
                     SGDELKKTLGERWSDWKPAAGQTWHSFNDYINFSDKAGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIDGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRYGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDISGKSA
                     ASVAHWQKISQFRARHPAIGVGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11712..12965
                     /gene="avtA"
                     /locus_tag="J1Z28_003048"
     CDS             11712..12965
                     /gene="avtA"
                     /locus_tag="J1Z28_003048"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBA7717862.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13117..13590)
                     /locus_tag="J1Z28_003049"
     CDS             complement(13117..13590)
                     /locus_tag="J1Z28_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBA7717863.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13692..14540)
                     /gene="yiaJ"
                     /locus_tag="J1Z28_003050"
     CDS             complement(13692..14540)
                     /gene="yiaJ"
                     /locus_tag="J1Z28_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBA7717864.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
BASE COUNT         3703 a         3707 c         3585 g         3602 t
ORIGIN      
        1 ggggataacg tttacgccat taatggcaga agttgctgat agaggcgacg gaacgtttct
       61 cgtcgtggct gataagcggc ataacgctgc gcatctggta gatgcgactg ttctaacggt
      121 agttgcggca acaactcaat gagcgatttc tctggattcg ccgcaatctg cgccagcctt
      181 gctgcgccca gtgctggccc cacatcgcca cccgtgcggt aatccagttg ctgaccgctg
      241 atgtccgcca gcatttgacg ccagtactca ctacgcgccc cgccaccaat caacgtaaca
      301 ctttgcggtt taatgccgca ggcatgcacg acatccatgc catctgccag cgcataaccc
      361 acgccttcca gcactgctcg cgccagttca ttggggccat gttgatgagt caaaccaaag
      421 aaaacccctt tcgcctgggg attattgtgt ggtgtacgct cgccggaaag ataaggcaga
      481 aaccaaactg gctcggcact ttcatcagcc tgttgagctg cagtgattaa agcagggaca
      541 ttgctcaggc cggttaattt cgcggcccaa tccagacacg aagccgcact cagcattaca
      601 gacattaaat gccaacgttg cggtaacgca tggcaaaagc tatgtacggc gctttctggc
      661 ttgcttaaga acccttcgct gacagcaaaa tagacccccg acgtccccag cgataacatt
      721 gcctgattag catcaaccat tcccacacca accgcaccag ctgcattgtc gccaccgcct
      781 gcgataactg gcaccgccgc cataccccac gctttcgcaa cttcaggtaa caaagcacca
      841 gtaatttcgc tgccttcgta taatgcgggc atctggtcac gagataagtg gcaagcctgc
      901 agcatgagat cgctccagtc acgctttgcg acatccagcc acatagtgcc agctgcgtca
      961 gacatatcgc tggcaaactc ccccgtcata cgcagacgca agtaatcttt cggtaataat
     1021 accttatcga tttgacggaa tatctccggc tcatgccgct gaacccatag caatttaggc
     1081 gcagtaaatc cgggcatcat caggttgccg gtaatcaccc gcgattgcgg aactcgcgct
     1141 tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc acaaaatggc agggcgtaac
     1201 acccgttgct gagcatccag taaggttgca ccgtgcatct gtccggcaat acctaatgct
     1261 ttaacgtctt gcagagaatg ctgaacgccc agagctttca ttgcgcgatc agttgcctgc
     1321 caccactgtt ccgggtcttg ttccgaccag agtggatgcg ggcgcgaaac ggtcagcttt
     1381 tccgtttgcg cagcaaccac ctcaccctgc tcgttgagca aaataacttt tacgcccgag
     1441 gtgccaagat ctatcccgat atacatatcg atcgttcctt aaaaaaatgc ccggtatcgc
     1501 tactgataac cgggccaacg gactgcacag ttagctgtta tttgtcgaac agataatgat
     1561 ttaccagatt ttccagttgc tcctggcgac cactctgatg caccggagac aaattatgtt
     1621 cctgagcata tttggctaaa tctgccagtg acatttggcc tttcaggatt tgctggccta
     1681 attcactatt ccagccggaa taacgctgcg cgatgcgttt atccagctcg ccatcttcaa
     1741 tcatgcgcgc tgcaattttc agcgccaacg ccatcgtatc catcgcgccg atatgaccgt
     1801 aaaacagatc atatttatca gtactttgac gacgtacttt ggcatcgaag ttcagaccac
     1861 cggtggtgaa accgcctgct ttgagaattt catacatcac cagcgcattc tcttccacac
     1921 tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc gccacggttg gcgtcgacag
     1981 aaccgaacag gccaagcgca atggcggtgg ctatttcatg atggaaagag tgacctgcca
     2041 gcgtcgcgtg gttagcttca atgttcagtt taatctcttt ttccagacca aactgtttca
     2101 ggaagccata gaccgtcgca gcatcgtaat catattgatg tttggtcggt tcttgcggtt
     2161 tcggttcgat aagcaacgtg ccctggaaac cgattttatg tttatgctca accaccatct
     2221 gcataaagcg gcccagctgt tcacgctcct ggcgcaagtc ggtatttaac agcgtttcgt
     2281 aaccttcacg accaccccac aggacatagt tttcaccgcc caatttatgg gttgcttcca
     2341 tcgctgtaac aacttgcgtt gccgcccaac tgaagacttc aggatctggg ttcgtcgccg
     2401 cacccgcgcc atagcgaggg tttgtaaagc agttagcagt tccccacagc agcttcacgc
     2461 cgctctcttc ttgcttgcct gccaggacat caaccatttg cgcaaaatta ttgatgtact
     2521 cttttaacga cgcgccctca ggggaaacat ccacatcgtg gaagcaataa aatggcacat
     2581 gtaacttgtg gaaaaactca aatgcgacat ctgctttacg cttcgccaac gccagtgcct
     2641 caccaggctg ctgccacgga cgattaaacg cccccacacc aaacatatcc gccccgttcc
     2701 agcagaaggt gtgccagtag caggcggcaa aacgcaagtg ctcttccata cgcttgccca
     2761 acaccagttc gtcgggattg tagtgacgga atgctaacgg gtttgaggat tttgagcctt
     2821 cataacgaac gcgatcgagc tggtcaaaat aggcttgcat attgaactcc ataatcaggt
     2881 aatgccgcgt gtgatggatg atatcgtaat attgggcact ccctttcagt tgctcaatta
     2941 tgttatttca cactgctatt gagataattc acaagtgtgc gctcgctcgc aaaataaaat
     3001 gggatgatga aactgggtaa ttcctcgaag agaaaaatgc aataagtaca attgcgcaac
     3061 aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa aatcgtaatc gaaagataaa
     3121 aatctgtaat tgttttcccc tgtttagttg ctaaaaattg gttacgttta tcgcggtgat
     3181 tgttacttat taaagctgtc ctctaactac agaaggccct acaccatgaa aataaagaac
     3241 attctactca ccctttgcac ctcacttctg cttaccaacg ttgcggcaca cgccaaagaa
     3301 gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac gctggcaaaa agatcgagat
     3361 atttttgtga aaaaagcaga atctctcggc gcgaaagtat ttgtacagtc tgcaaatggc
     3421 aatgaagaaa cacaaatgtc gcagattgaa aacatgatta accggggtgt cgatgttctt
     3481 gtcattattc cttataacgg tcaggtatta agtaacgttg taaaagaagc caaacaagaa
     3541 ggtattaaag tattagctta cgaccgtatg attaacgatg cggatatcga tttttatatt
     3601 tctttcgata atgaaaaagt cggtgaactg caggcaaaag ccctggtcga tattgttccg
     3661 caaggtaatt acttcctgat gggcggctcg ccggtagata acaacgccaa gctgttccgc
     3721 gccgggcaaa tgaaagtatt aaaaccttat gttgattccg gaaaaattaa agtcgttggt
     3781 gaccaatggg ttgatggctg gttaccggaa aacgcattga aaattatgga aaacgcgcta
     3841 accgccaata ataacaaaat tgatgctgta gttgcctcaa acgatgccac cgcaggaggg
     3901 gcaattcagg cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat
     3961 gcggatcttg caggtattaa acgtattgct gccggtacac aaactatgac ggtgtataaa
     4021 cctattacat tgttggcaaa tactgccgca gaaattgccg ttgagttggg caatggtcag
     4081 gagccaaaag cggataccac actgaataat ggcctgaaag atgttccctc ccgcctgctg
     4141 acaccgatcg atgtgaataa aaacaacatc aaagatacgg tagttaaaga cggattccac
     4201 aaagagagcg agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca
     4261 tgccgcgtga attgattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaaa
     4321 atattaccaa aaccttcggc agtgtgaagg cgattgataa cgtcagcttg cggttgaatg
     4381 ctggcgaaat cgtctcactc tgtggggaaa atgggtcagg taaatcaacg ctgatgaaag
     4441 tgctgtgtgg tatttatccc catggttcct acgaaggcga aattattttt gcgggagaag
     4501 agattcaggc gagtcacatc cgcgataccg aacgcaaagg tatcgccatt attcaccagg
     4561 aattggcgct ggtgaaagaa ctgaccgtgc tggaaaatat cttcctgggt aacgaaataa
     4621 cccataatgg cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac
     4681 aggtcagttt atccatttca cctgataccc gcgttggcga tttaggactt gggcaacaac
     4741 aactggttga aattgccaag gcacttaata aacaggtgcg tttgttaatt ctcgatgagc
     4801 cgacagcctc attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac
     4861 aacagcacgg tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt
     4921 ccgatacgat ttgcgttatt cgcgacggtc aacacattgg tacgcgtgat gctgctggaa
     4981 tgagtgaaga cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta
     5041 atgaaccaca taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc
     5101 cggttaatcg tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa
     5161 tactgggtat cgccggactc gttggtgccg gacgtaccga gactattcag tgcctgtttg
     5221 gtgtgtggcc cggacaatgg gaagggaaaa tttatattga tggcaaacag gtagatattc
     5281 gtaactgtca gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg
     5341 acggcatcgt tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaaaaaat
     5401 ttaccggtgg cattagccag cttgatgacg cggcagagca aaaatgtatt ctggaatcaa
     5461 tccagcaact caaagttaaa acgtcgtccc ccgaccttgc tattggacgt ttgagcggcg
     5521 gcaatcaaca aaaagcgatc ctcgctcgct gtctattact taacccgcgc attctcattc
     5581 ttgatgaacc caccaggggt atcgatattg gcgcgaaata cgagatctac aaattaatta
     5641 accaactcgt ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc
     5701 tcggccttag cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa
     5761 atcataacct gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg
     5821 aaaagcaatc cgtctgaagt gaaattagcc gtaccgacat ccggtggctt ttccgggctg
     5881 aaatcactga atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc
     5941 tttacctgga ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc
     6001 caaaccgcga ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa
     6061 atcgaccttt ccgtcggctc aatgatgggg ctgttaggtg gcgtcgcggc gatttgtgac
     6121 gtctggctag gctggccttt gccacttacc atcattgtga cgttggttct gggactgctt
     6181 ctcggagcct ggaacggatg gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc
     6241 ctcgcgggca tgttggcatt tcgtggcata ctcattggca tcaccaacgg cacgactgtt
     6301 tcccccacca gcgccgcgat gtcacaaatt gggcaaagct atctacctgc cagcaccggc
     6361 ttcatcattg gcgcgcttgg cttaatggct tttgttggct ggcaatggcg cggaagaatg
     6421 cgccgtcagg ctttgggttt gcagtctccg gcctctaccg cagtagtcgg tcgccaggct
     6481 ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga atgattaccg tggtgttccc
     6541 actcctgttc tgctgctaac gttactgtta ttgggcggaa tgtttatggc aacgcggacg
     6601 gcatttggac ggcgcattta tgccatcggc ggcaatctgg aagcggcacg tctctccggg
     6661 attaacgttg aacgcaccaa acttgccgtg ttcgctatta acggattaat ggtagccatc
     6721 gccggattaa tcctcagttc tcgactcggc gctggttcac cttctgcggg aaatatcgcc
     6781 gaactggacg caattgcagc atgtgtgatt ggcggcacca gtctggcagg cggcgtcggg
     6841 agcgtggctg gagcggtaat gggggcattc atcatggctt cactggataa cggcatgagt
     6901 atgatggatg taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggct
     6961 gtatggatgg actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc
     7021 cggtaacgta ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta
     7081 ctaaacgtca ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag
     7141 tagaaggcgt aggggaatat ttacaggcgt cacaatcgga atgggatatt ttcattgaag
     7201 aagatttccg cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg
     7261 acttcgacga caaacaaatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg
     7321 ttggtggctc gtatcacctt gccgaaagtt acccacccgt tcattacatt gccaccgata
     7381 actatgcgct ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg
     7441 ctttttatgg tcttccggaa tcaagcggta aacgttgggc cactgaacgc gaatatgcat
     7501 ttcgtcagct tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg
     7561 cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac
     7621 cgcaaaccgg gattattgcc gttactgacg cccgggcgcg gcatattctg caagtatgtg
     7681 aacatctaca cattcccgta ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac
     7741 tgacccgcta tctttcgcgc gtcgctctct cttctgtcgc tcagggcgca cggcaaatgg
     7801 gctatcaggc ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc
     7861 ggattttggt cccgccagtc cgcgtgattg aacgacgttc aacggattac cgctcgctga
     7921 ccgatcccgc cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta
     7981 aagtggatca ggtactggat gcggtgggca tatcacgctc caatcttgag aagcgtttta
     8041 aagaagaggt gggcgaaacc attcacgcca tgattcatgc cgaaaagctg gagaaagcgc
     8101 gcagcctgct gatttctact accttgtcaa ttaacgagat atcgcaaatg tgcggttatc
     8161 catcgctgca atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt
     8221 atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg agaattatcg gcgtcgcatc
     8281 aggtaactca gcacaaccac ctgatacgag caaaagcctt atccgcctga caagtcggat
     8341 aaggctcgag gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca
     8401 ttgcgaacag gtagttgtta taactcttcc ctttggtcga gtagcccttc agcttgtgaa
     8461 tcatcgctgt ggcagtcact tcctgatccg ctttacgcag ttgtgcacgc gatttacgga
     8521 acgaagagta agccgggtgc gtattgaggt tagtgacata ggcgctcacc gattctttga
     8581 cagaactaaa ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca
     8641 tgcatttcat gccgaacagg ttgttgttgt tgcgcgccag ctttgacgtt ccccaaccgc
     8701 tttctgctgc agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca
     8761 aggtattcca cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga
     8821 tgtctttcag acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca
     8881 gccagttacg ttccgcagta atggccgcat tttggctggt aatgtaaggc attacagtcc
     8941 ggagaaacgc ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac
     9001 tactctttgc actattgcga gaatactctt gtttactgct tacctgttta ttactagctt
     9061 tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg
     9121 aaaacaccag agtgagtaac ataagcatca tcgccccata tcgtcgtatg ggagtcaaaa
     9181 tcatcaggtc tcctggtcgg atttaatcat tccaacacct tatatttttt acaaatttga
     9241 gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa
     9301 atctgaaact atgtcacgag ttaacgattc agattgacgc taaatcgcag aaaatgtggg
     9361 gtttatcgca aaattcagcc gttttttgcg cgcgatcgct cacccttgct tctcatcctg
     9421 tggacttacc gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc
     9481 gcagcaataa ggactcatcc gccatgaaac tcgccgcctg ttttctgaca ctccttcctg
     9541 gcttcgccgt tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggga
     9601 cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac gcgtccacta atgctaaatt
     9661 ttgaccaaca gtgctggcag cctgcggatg cgataaaact caatcagatg ctttccctgc
     9721 aaccttgtag caacacgccg cctcaatggc gattgttcag ggacggcgaa tatacgctgc
     9781 aactagacac ccgctccggt acgccaacat tgatgatttc catccagaac gccgtcgaac
     9841 cggtagcaag cctggtccgt gaatgcccga aatgggatgg attaccgctc acactggatg
     9901 tcagcgccac ttttacggag ggagccgccg tccgggatta ttacagccag caaattgcga
     9961 tagtgaagaa cggtcaaata acgttacaac ccgctgccac cagcaacggt ttactcctgc
    10021 tggaacgggc agaaactgac acatccgccc ctttcgactg gcataacgcc acggtttact
    10081 ttgtgctgac agatcgtttc gaaaacggcg atcccaataa tgaccagagt tacggacgtc
    10141 ataaagacgg tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgacca
    10201 acaaactgga ttacctccag caactgggcg tcaatgcttt atggataagc gccccatttg
    10261 agcaaattca cggctgggtc ggcggcggta caaaaggtga tttcccgcat tatgcctatc
    10321 atggttatta cacacaggac tggacgaatc ttgatgccaa tatgggcagt aaagccgatc
    10381 tacggacgct ggttgatagc gcgcatcagc gcggtattcg tattatcttt gatgtcgtga
    10441 tgaaccacac cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat
    10501 acctttctgg tgacgagctg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg
    10561 ccgccgggca aacctggcat agctttaacg attacattaa tttcagcgac aaagcaggct
    10621 gggataaatg gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg
    10681 gattcgacga tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta
    10741 ccgcttctgg tctgccagtg ttctataaaa acaaaacgga tacccacgct aaagtcatcg
    10801 acggctttac ccctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg
    10861 ggattgatgg ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac
    10921 tgaaaaccga agccagcgct gcacttcgcg aatggaaaaa agctaacccc gacaaagcat
    10981 tagatgacaa acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg
    11041 actactatcg ctacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga
    11101 aagcagtcga ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc
    11161 aggatttcaa cgtgttgagc tacctctcgt cgcatgatac ccgcctgttc cgtgaagggg
    11221 gcgacaaagc agcagagtta ttattattag cgccaggcgc ggtacaaatc ttttatggcg
    11281 atgaatcctc gcgtccgttc ggtcctaccg gttctgatcc gctgcaaggt acgcgttcgg
    11341 atatgaactg gcaggatatt agcggtaaat ctgccgccag cgtcgcgcac tggcaaaaaa
    11401 tcagccagtt ccgcgcccgc catcccgcaa ttggcgtggg caaacaaacg acactttcgc
    11461 tgaagcaggg ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg
    11521 cggggcaaca gtgacttttc cggcttcccg ttcgtgagta cctcgggaag ccgccaacca
    11581 ggataaaatg tcagccctaa tcagcattgc aggataaagc accgctcact cttcaacaga
    11641 ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca
    11701 gatatcccgc tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta
    11761 cgctgctgat ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg
    11821 gcggtaatcc ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca
    11881 tgctggaaag tggcaaagcg actgatgcac tgtgcaatta cgacggtcca caggggaaaa
    11941 cggagctact cacactgctt gccggaatgc tgcgcgagaa gttgggctgg gatatcgaac
    12001 cacagaatat tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaatctgt
    12061 ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat
    12121 atattggcta cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata
    12181 ttgaactgct gccggaaggc cagtttaaat accacgttga ttttgagcat ctacatattg
    12241 gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta
    12301 ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga
    12361 ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt cagcgaagcg cgcccgctat
    12421 ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct ggggctacct ggctcccgct
    12481 gcggcattat catcgccaat gaaaaaatca tcaccgccat caccaatatg aacggcatta
    12541 tcagcctggc acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg
    12601 atctgctgcg cttgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa
    12661 ctatcgccat cattcgccgc tatttaccgg aagatcgctg cctgattcat aaaccggaag
    12721 gagccatttt cctctggcta tggtttaagg atttgcccat tacgaccgag cagctctacc
    12781 agcgcctgaa agcacgcggc gtgctgatgg tgccggggca caacttcttc ccgggactgg
    12841 ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga
    12901 aaattgaggc gggtgtgaag attctggcag aagagataga aagagcctgg gctgaaagtc
    12961 actaaaaatt gcctgatgcg ctacgcttat caggcctaca tgatttctgc aatatattga
    13021 atttggtaga atttgtaggc cggataaggc gttttagccg catccggcat aaacaaagcg
    13081 cactatccct gcgttgagaa acgccggaaa aacgtttcaa acaggctgct gccgtagccg
    13141 atgtcgctgc actttctcga catccatgca ctgcaacgcc tttgtcgggc aggcttcaac
    13201 acaggccggc cccgtcgccc gatgccagca cagatcgcac ttaatcgccc tcgccttttt
    13261 gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc agcatacagc ttttacagcc
    13321 aatgcaacgt gattgttcaa cgaaaatatg cccatgttcg cggcttatcg cgtcaacagg
    13381 gcacacattc gcgcacggtg catcttcaca ctgatgacag gctactgccg tggtccagct
    13441 gtggtcttta atgacacgaa tacgggaaat aaactcgtct ggtgacaacg cagcgcaatc
    13501 ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag gtacggcaac cgatacattt
    13561 cgtcgcatcc gcaataataa accggttcat gatcactcct ttgcactaaa acagcagagt
    13621 gccagagagc gcgtccctac tgctttgatc caacgaagga aatgttaaag ttttggcact
    13681 tgtccaaagg gttatgtaat cgcgccctgg tcgtccctga cagtaaatcc cagttcatta
    13741 gaaatagcct gcgcggtttc acgcagtggt ttcaggagat ttttctctcc cacctgtttc
    13801 agacgtgatg tcgaaagcga aatcgacacg gcgtacggca cccgcccatg aatatcaaac
    13861 accggaacag caatacagga gacgccgagt tcgttttctt ctctgtccat cgccgctcca
    13921 ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca gctcggtaat ggtattgcgg
    13981 gttaacggct ggatctcatg ctgatggctt tcccagtatg acttcacata gtccgggtga
    14041 ccaaacgcca tgtagatctt gcccattgcg gaacagtaga gcggcatatg ctggcctata
    14101 taggcacggg ttcgcagcat cccggttgtg ggttccagct tataaatcaa aatagcgtga
    14161 tcgtcttcgc ggctggagaa gttaatagtt tcaccagtgg cgatgttcag agcctcaaga
    14221 tgcggagcgg cgatatgaat gatattcagc gaagatagcg ccttctgccc gacggcaata
    14281 aatttggtgg tcaggcgata actccctgcg gcgggcgcgg tggtcacata gccgcaggac
    14341 tgtaatccct gcaataagcg atggacggta ctcttattta aaccagccag ctccgaaaga
    14401 tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct caatcagcat caacccacga
    14461 aacagactct ggcttccggc tgggcgctct ttttcctgcg ccatctcgtt ctcttttttt
    14521 cccatcactt ctttccccat tttgtcgcgt cctgatggta gcgcaaagtg tgccgtagtt
    14581 cacgatctcg acagata
//
LOCUS       DADSZD010000109        14571 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.110, whole genome shotgun sequence.
ACCESSION   DADSZD010000109 DADSZD010000000
VERSION     DADSZD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.110"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(378..2042)
                     /gene="asnB"
                     /locus_tag="J1Z28_003051"
     CDS             complement(378..2042)
                     /gene="asnB"
                     /locus_tag="J1Z28_003051"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBA7717865.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTINEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNSKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2298..3050)
                     /gene="nagD"
                     /locus_tag="J1Z28_003052"
     CDS             complement(2298..3050)
                     /gene="nagD"
                     /locus_tag="J1Z28_003052"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBA7717866.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(3098..4318)
                     /gene="nagC"
                     /locus_tag="J1Z28_003053"
                     /gene_synonym="nagR"
     CDS             complement(3098..4318)
                     /gene="nagC"
                     /locus_tag="J1Z28_003053"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBA7717867.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            complement(4327..5475)
                     /gene="nagA"
                     /locus_tag="J1Z28_003054"
     CDS             complement(4327..5475)
                     /gene="nagA"
                     /locus_tag="J1Z28_003054"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBA7717868.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            complement(5535..6335)
                     /gene="nagB"
                     /locus_tag="J1Z28_003055"
     CDS             complement(5535..6335)
                     /gene="nagB"
                     /locus_tag="J1Z28_003055"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBA7717869.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            6668..8614
                     /gene="nagE"
                     /locus_tag="J1Z28_003056"
     CDS             6668..8614
                     /gene="nagE"
                     /locus_tag="J1Z28_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBA7717870.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            complement(8703..9983)
                     /locus_tag="J1Z28_003057"
     CDS             complement(8703..9983)
                     /locus_tag="J1Z28_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane transport protein"
                     /protein_id="HBA7717871.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(9997..10467)
                     /locus_tag="J1Z28_003058"
     CDS             complement(9997..10467)
                     /locus_tag="J1Z28_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBA7717872.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            complement(10464..11633)
                     /locus_tag="J1Z28_003059"
     CDS             complement(10464..11633)
                     /locus_tag="J1Z28_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HBA7717873.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVALF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     ERRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            complement(11669..12427)
                     /locus_tag="J1Z28_003060"
     CDS             complement(11669..12427)
                     /locus_tag="J1Z28_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBA7717874.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERRNKKVSTLIETHESHSH"
     gene            complement(12487..13374)
                     /locus_tag="J1Z28_003061"
     CDS             complement(12487..13374)
                     /locus_tag="J1Z28_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBA7717875.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSACISGLANIAPREI
                     NAIWSRFHASDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            complement(13378..14535)
                     /locus_tag="J1Z28_003062"
     CDS             complement(13378..14535)
                     /locus_tag="J1Z28_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBA7717876.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVSVIDNVPAEPTHHDVRQQMATLGNASFDVVVGVGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSAEEKSHALVTWLQALVNQLKLPNNL
                     AALGVPPEDIASLSEAALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
BASE COUNT         3541 a         3752 c         3755 g         3523 t
ORIGIN      
        1 cttttttttc gcgatacctt atcggcgttg cggggcgcat tatgcgtata gagccttgca
       61 gcgtcaacct ctttttcaag gaaaattgct cgaaagtgac tgtttggtta ggttgcgaac
      121 agcatggcgc tatattcgtc aattattgtt tactttgtgt tttttcccca ccctacagcc
      181 attcttttgt catacaggat gaaattcgga attcaacaat agtggtggga aattaatcta
      241 tgaaatactg gcctacagta atgagttgtc aaacagcgat gtggcaaacc cggacatttc
      301 cttactgcat atcagaatca acaagcgccc cagtaactga aattgccccg gatttctccg
      361 gggcaatatt acacgtctta tttatatgcc gactggtgaa cgccaaccgc gcgaccagac
      421 ggatcgtcca ttttcttgaa cgcttcatcc cactcaatcg ctttagcgga agaacaagcg
      481 acggaaggac cgcccggcac gcactcagcg gcgctcggaa gcgggaacaa ttcttcaaag
      541 atctcccggt acagatacgc ttcttttgag gtcggcgtgt tgtacgggaa gcggaagcgg
      601 gcagtttcca gctgttgatc agaaacctgc tgcgctgcca cttctttcag ggtgtcgatc
      661 caactgtaac cgacgccatc ggagaactgc tctttttgcc gccaggccac gcttgcaggc
      721 agatacgctt caaaacattc acgcaggatg tgtttttcca ttttgctgtt accgcacatt
      781 ttatcctgtg ggttaatgcg catcgccaca tcaaggaatt ttttgtcgag gaacggaacg
      841 cgtgcttcca cgccccaggc tgacatcgct ttgttggcac gcgcgcagtc atacatatgc
      901 agggccagca gtttacgcac cgtctcttca tgcaactctt tagcgttcgg cgctttgtgg
      961 aagtatagat aaccgccaaa cacttcgtca gaaccttcac cggacagcac cattttaatg
     1021 cccatcgcct tgatcttacg cgacattaaa tacatcggtg ttgaagcgcg aatagtggtc
     1081 acatcataag tttcgatgtg gtaaatcacg tcgcggatgg catccagacc ttcctgtaca
     1141 gtgaagtgaa tttcgtgatg caccgtgccc agatggtttg ccacttcctg ggctgctttc
     1201 agatccggtg aacccggcag acctacagca aaggagtgca actgcggcca ccaggcttca
     1261 gagcgttcct gatcttccac gcgacgggcg gcgtatttct tggtgatagc ggaaataatt
     1321 gaggaatcca gaccaccaga aagcaacaca ccgtaaggca catcagacat cagatggctt
     1381 ttaacggaat cttccagtgc ctgacgcagc tcgtttttgt cggtcacgtt atcttttacc
     1441 gcatcgtagt cgaaccagtc gcgatgataa taagaacgga tttcgccgtc ctgactccac
     1501 aaatagctcc ccgccggaaa ctcgttaatc gtgcggcaaa ctggcacgag ggctttcatt
     1561 tctgaggcca catacagctg accgtgttcg tcatagccca tatacagtgg gatgatcccc
     1621 agatggtcgc gaccaatcag atatgcatct ttttcgctgt cgtacagggc aaaggcaaac
     1681 atgccctgca agtcgtcgag aaattccggc cctttttcct gatacagcgc gaggatcact
     1741 tcacagtcag acccggtctg gaactggtaa cgatcgccat attcagcgcg caatgcctgg
     1801 tggttgtaga tttcaccgtt tactgccagc acatgagttt tttgttggtt gtagagaggt
     1861 tgcgcccccg cgttaacgtc aacaattgac agacgttcgt gggcgagaat ggcgttatcg
     1921 ctggcataaa taccggacca gtccgggcca cgatgacgca tcaggcgtga cagctcgagt
     1981 gctttcttac gcagctcaac tgcgtctgtt ttgatatcga atacgccaaa aattgaacac
     2041 ataaccttct ccgttaacct ggtgtttact gcgtgttgtg tttgcttgtt aaaaaaatgc
     2101 cgcaaagcag cacggtgcgc aagcgatttg gcggtggaaa aataaaaaac gtaatggcga
     2161 ttgctgattg atgaaaaagg ggagggcttt tgggtatatt tattgatgga tcgataattt
     2221 ttgacgggtt ttattgaatg gggtgatttt aatgatgggt aaaaataaga aaccacgccc
     2281 tgaggcgtgg tttctattca gataacgtcg atttcagcga ctgatgggta aatccaactt
     2341 gggcggaacg gcatactgtc gatatcgtcg agcgacgaaa caccagaaag caccagaatc
     2401 gtctccaggc cggcctggaa gcccgccaga atatcggtac gcaagttatc gccgacaatc
     2461 accgtttctt ccgaatgcgc ctgcattttg tttaatgccg cgcggatgat ccacggactc
     2521 ggctttccaa catagaacgg tttgcgcccg gagattttct cgatccctgc acacaacgcg
     2581 ccacaggcgg gataaaaacc gcgcccgtgg gtgtccgggt tggtggcgat aaagcgtgca
     2641 ccgttagcga cgaaataggc tgctttatgc atcatgtccc agttgtagga acgcgtttcg
     2701 ccaacaatca caaaatcagg gttcacatcg gtaatagtga aaccggcttt gtacagttca
     2761 tgaatcagtg cgccttcgcc caccacatac gctttcttgc cttcctggcg acgcagaaaa
     2821 tcggcggtcg ccatcgcgga ggtataaaac acgctatccg gtacatcgac accggcagtg
     2881 gcaaagcgat tcgccagatc ttgcccggtc tgcgaaggat agttggtcag caacaccagc
     2941 ggcaggcctt tatccataat cccgtgcaaa aattccgctg cacccggtac ggcgacgtta
     3001 tcgtgcatca gcacgccgtc gatatcgcaa attacatttt taatggtcat ggactaccca
     3061 gaatattgac aacaataagc gccactataa aagcacatta attttccagc aaatgctgga
     3121 gcaaaatacc gttgagcatg gcgcgtttta ccagcgcaaa agcgccgatt gccgagcggt
     3181 gatccaactc agaacgtacc accggtagat tagtacgaaa cgccttcagc gcctgggtat
     3241 taatgcagct ttcaatagca gggagcagca ctttatcggc ttcggtgatt tctccagcaa
     3301 taacaatttt ttgcggatta aataagttga tagcaatggc gatggtttta cccagatgac
     3361 gaccgacata ttcaatcact tccgaggcca ggctatcgcc tttgttcgcg gctttgcaga
     3421 tagttttgat ggtgcagtcg tccagtggca cacggctctg gtagccctgc tttaacagat
     3481 tcaacacccg ttgttcaatg gcagcattgg cggcgatagt ttccaggcag ccaaagttgc
     3541 cgcagtggca gcgttcaccc agcggttcga cctgaatatg gccaatttca ccgacgttgc
     3601 cgttgcgccc gataaaaatt cgcccgttag agataatccc ggccccggtt ccgcgatgga
     3661 cacgcaccag aatggagtct tcgcaatcct gacttgcacc gaagtagtgc tctgccagcg
     3721 ccagactgcg gatatcgtgg ccgacaaaac aggtcacttt aaaacgctct tccagtgctt
     3781 caaccagccc ccagttttct acctgaatat gcggcatgta atgaattttg ccgctgtccg
     3841 ggtctacaag ccctggcagg atcaccgaaa tcgcgatcag ctcgcgtagc ttgcgctggt
     3901 agctatcaat aaactgagca atggcattca gcagggcatg ttccagcgtc tgctgggtac
     3961 gttccggcag cgggtaatgt tcttctgcca gcactttgct gctgagatca aacaaggtga
     4021 tggtggcgtc gtgacgacca agccgtacgc cgattgcgtg gaaattgcgg gtttcggtga
     4081 cgatggagat agcgcggcgg cccccggtgg aggcctgctg atcaacttct ttgatcagcc
     4141 cgcgttcgat aagctgacgc gtaattttgg ttacgctggc gggggcaagc tgactttgct
     4201 cggcaatctg aatccgcgag attggcccgt actggtcaat caggcgataa actgccgcgc
     4261 tgttaagctg ttttacgaga tcaacattac ctatctgagc ttgtccgcct ggtgtcatac
     4321 tttctcttat tgagttacga cctcgttacc gttaacgatg gtcctggtga ttttaaaatc
     4381 aggtgtgaat gcagtcaggt tggctacttt acctgcggcg agcgtgccga gacgtttctc
     4441 aacgccaatc gcacgtgccg gatagagcgt cgccatacgc agcacttcat ccagtgcgat
     4501 accacaatgt tcgaccagat tacgcacgcc ttcaatcatg gttaaggatg aaccgcttaa
     4561 cgtaccgttc tcatccacac aaagtccatt acggtagtat attgttttac ccgcaaaaat
     4621 gaactgttca atgttggcac ctgctggcgc ggtggcatcg gtaaccagac acaatttgtc
     4681 gcctttcaga cgtttagcgt tacgaatgtt ggcgtaatca acatgcaggc catcagcgat
     4741 aataccgcaa taaatgtcag cttcgtcgag gatcgcgccc gccaggcccg gttcacgacc
     4801 ggtaatatac ggcatcgcgt tgtacagatg ggtggcaaag gtaatccccg cgcggaaacc
     4861 ggctttcgct tctttcaacg tcgcgttgga gtgaccagca gaaaccacaa tcccggcatt
     4921 tgccagtttg ctgatgactt ccgctggaac catttccggt gccagagtca ctttggtaat
     4981 cacgtcggcg ttctcacaca ggaaatcgac cagcgcggca tcaggcttac gcacaaaatt
     5041 cggattatgg gtgccttttt ttaccagatt cagccacgga ccttccagat gcagacctaa
     5101 cgcctggttc ggatgttttg ccaggtactc gcgcataacg cgcacgccct gtttcatcag
     5161 ctcatcgctg gtggtgataa gcgtcggcag atagttagta cagcctgatt tctcattggc
     5221 tttctgcatg atttccagcg tttccacgct gaccgcttca gcggtgtcgt taaactgtac
     5281 gccgccgcag ccgtttaact gcacatcgat aaaaccgggg gagagaatgg ccccgttcag
     5341 tgaacgttgt tcgatctctg gcggcagttc cgctaccgga cagacgcttt taatcaggcc
     5401 atcagcgata acaaccgcgt ggtcatcaag aaattcgtgg ccggtaaaga tccggccctg
     5461 ggttaatgca tacattctga cccccgattt taaaaaataa tgccctgagc aaggagccag
     5521 ggcagggata acaattacag acccttgata ttttctgctt ctaattcatt gaaatatctt
     5581 aaagtcttaa ctttcagctc catggtggaa ggttcatcgc acaccatgat cgcttttgga
     5641 tgcagttgca gacaactgat ggtccacatg tggttcacgc aaccttcaac ggcggcctgc
     5701 agtgccagtg ctttctgact acccagcacc agaatcatca cttcttcggc atccagcaat
     5761 gtaccaacac cgacagtcag ggcatatttt ggcacctgat taacatcgtt atcaaagaaa
     5821 cgagagttcg cgacgcgagt gtcatgagtc agggttttga tacgagtacg agaagccaga
     5881 gaagacgccg gttcgttaaa tgcaatatga ccgtcgttac ctacaccgcc cataaacaga
     5941 tgaatttttc cgtaagaacg gattttttct tcatactggc ggcattcggc gtcgatatcc
     6001 ggggcgttgc cgttgagaag gttgatattt tctgctggaa tatcaacgtg atcgaagaaa
     6061 ttacggtgca taaagctgta gtagctttcc ggatgctctt tcggcagacc gacatattcg
     6121 tccatgttga aggtgacaac gtgcttaaag ctgacctggc ctgctttatg catttcgact
     6181 aacgctttat aggtggtcat cggcgtgccg ccagtcggca ggcccagtac aaacggacga
     6241 tcggcagtcg gtttgaacgc attgatacga ttgacgatat ggcgagcagc ccatttgccg
     6301 acctgttcag cggtagtcag ggggatcagt ctcattattc acctcaataa gtaaaatgta
     6361 agccgttggc ggattaggca tctttaagcg taacctggat ttgcgcagac aggcgtcaat
     6421 ccgacctgat tttttgaatg ataaaataag ttttctggtt tagccagtaa aagggagtga
     6481 tgataacgat atttggtgac aaaactcaca aaagacacgc gtttaatttg cgatacgaat
     6541 taaattttca cacactctgt agcagatgat ctaacaatct gattacagaa catcggcagt
     6601 acaatttgca gcaaaataaa aatacggctt taaacgagcc aaatagggtt ctcgtagggg
     6661 gaataagatg aatattttag gttttttcca gcgactaggt agggcgttac agctccctat
     6721 cgcggtgctg ccggtggcgg cgctgttgct gcgattcggt cagccagatt tacttaacgt
     6781 tgcgtttatt gcccaggcgg gcggtgcgat ttttgataac ctcgcattaa tcttcgccat
     6841 cggcgtggca tccagctggt cgaaagacag cgcaggtgcg gcggcactgg cgggtgcggt
     6901 aggttacttt gtgttaacca aagcgatggt gaccatcaac ccagaaatta acatgggtgt
     6961 actggcgggt atcattaccg gtctggttgg tggcgcagcc tataaccgtt ggtccgatat
     7021 taaactgccg gacttcctga gcttcttcgg cggcaaacgc tttgtgccga tcgccaccgg
     7081 cttcttctgt ctggtgctgg cggccatttt tggttacgtc tggccgccgg tacagcacgc
     7141 tatccatgca ggcggcgagt ggatcgtttc tgcgggcgcg ctgggttccg gtatctttgg
     7201 tttcatcaac cgtctgctga tcccaactgg tctgcatcag gtgctgaaca ccatcgcctg
     7261 gttccagatt ggtgaattca ccaacgcggc gggaacggtt ttccacggtg atatcaaccg
     7321 cttctatgcc ggtgacggca ccgcggggat gttcatgtcc ggcttcttcc cgattatgat
     7381 gttcggtctg ccgggtgcgg cgctggcgat gtacttcgca gcaccgaaag agcgtcgtcc
     7441 gatggttggc gggatgctgc tttctgttgc tgttactgcg ttcctgaccg gtgtgactga
     7501 gccgctggaa ttcctgttca tgttccttgc accgctgctg tacctcctgc acgcactgct
     7561 gaccggtatc agcctgtttg tggcaacgct gctgggtatc catgccggct tctctttctc
     7621 tgcgggggct atcgactacg cgttgatgta taacctgccg gccgccagcc agaacgtctg
     7681 gatgctgctg gtgatggggg ttgtcttctt cgctatctac ttcgtggtgt tcagtttggt
     7741 tatccgcatg ttcaacctga aaacgccggg tcgtgaagat aaagaagacg agatcgttac
     7801 tgaagaggct aacagcaata ctgaagaagg tctcaatcaa ctggcaacca actatattgc
     7861 tgcggttggc ggtactgaca acctgaaagc cattgatgcc tgtattactc gtctgcgcct
     7921 taccgtggtt gactcagctc gcgtcaacga tgcgatgtgt aaacgtctgg gtgcttctgg
     7981 ggtagtgaaa ctgaacaaac agactattca ggtgattgtt ggcgcgaaag cagaatccat
     8041 cggcgatgcg atgaagaaag tcgttgcccg tggtccggta gccgctgcgt cagctgaagc
     8101 aactccggca actgccgctc ctgtagcaaa accgcaggct gtaccaaacg cggtatctat
     8161 cgcggagctg gtatccccga ttaccgggga tgttgtggca ctggatcagg ttcctgacga
     8221 agcattcgcc agcaaagcgg tgggtgacgg tgtggcggtg aaaccgacag ataaaatcgt
     8281 cgtatcacca gccgcaggaa caatcgtgaa aatcttcaac accaaccacg cattctgtct
     8341 ggaaaccgaa aaaggcgcgg agatcgtcgt ccatatgggt atcgacaccg tagcgctgga
     8401 aggtaaaggc tttaaacgtc tggtggaaga gggcgcgcag gtaagcgcag ggcaaccgat
     8461 tctggaaatg gatctggatt acctgaacgc taacgcccgt tcgatgatta gcccggtggt
     8521 ttgcagcaat atcgatgatt tcagcggcct tatcattaaa gctcagggcc atgttgtggc
     8581 gggtcaaaca ccgctgtatg aaatcaaaaa gtaatctgct ttatgtctga tgcgacgcgg
     8641 cagcgtcgca tccaacaata aaaagcggca gggatcttct ctgccgcttt ttttgtctga
     8701 gattacattg ggaagataaa aacagcgcct gcacaaagcg ccagaatgcg gatgagatac
     8761 attggtacag agaacaccca gaattcggga agtttgtatt tccccattcc gaggatcatg
     8821 gctggcattc cgtcaatagg catatagccg ccattccagc cagaaatcac caccgcaatc
     8881 gcggcagcgg tcgggttaag cccaaggcta gtacaggcgg caatggcgat gggggcaaaa
     8941 atatatactg accccatatt cgaacccgtg aaggtggcgc aggtactggt aagcaaagca
     9001 aaggcgacaa taaatacaaa cggtgagacg ccagaaccga gcacacctgc aacggcatcg
     9061 ccaaccatcg ccgtaaagcc ggtgcccgcc agtgcatcag ctacaccgat cacccccgcc
     9121 atcattaaaa tgaccggagc ccccatatga tcgcgaactt ctttgaagtc cagtacatcg
     9181 atcatcagaa caaacgcacc cgcaagaccc ggaataacga aggcgatatt tcccagaatg
     9241 ttcattagca tcatgccgat aacgctgaca gcgaatgcgg caatagtgca atattctttc
     9301 caggcaggaa gggcgttaaa cgtttcctcg cgtgccgcca gcgcatcctc ggaagcatcc
     9361 gcaatgggat gatccggaag aaaacggtag gcaataagac tccatgcgag gaaagccgct
     9421 gacatgatga gattcaccag cgcaaagcgc cccatcgaaa tctcctgatg aaaccccgcg
     9481 gtttcgagta cggtcgccgt aacgccaaag agaagcgcga gattgatagg aagaagcggg
     9541 tggttggtgg caaatcccag gggcatcatt aatttcgatg ttggtagctt tttactgtac
     9601 ggaatggtgg ataccagcga cagaattagc acgtaataac cggtcgctcc ggtgccggca
     9661 agactggcac ccagcataac aatgactaac aacagcccat agctgcggta actgccttta
     9721 ttgaccaggc ttatcatcgc agatttaacg ttgctgataa gccgggtttt ttgtagcgcc
     9781 gccatgacca ccataaaccc tgcaatcatc accacgcttg gattgacgaa tccggcaaaa
     9841 gcctgttgaa cggtcgaaag tcccgataca accagcaata aacacgcaag cagtggcggt
     9901 gcgccaaaag gcattttgtc cgacatgatc agcacgatca tcaggaccag tattcctaat
     9961 gccaggatca tttccattgt cattttttct ctccttttag gcttccagca tgtcgcgcgg
    10021 tactttaaag accgctttgc gtattgaagt agccgtgcca attgcgcaca gcgcctggtg
    10081 aggttcctca ggcataaaga cggcgaaatc accggcgtgt agggtgatgg cgacactgtt
    10141 ttcaggggat ggggcgataa acagatcggg cttacgttct tcatcgtttt cgcgagcgat
    10201 gggccgcgtt cccgcattaa tgccctcaca accagtaatc accacctgta tatcggccca
    10261 taaatggtga tactccgtat ggcggagttc ggcgggttgc gtgtctgaag ttcccagcgt
    10321 gcagaaccac cgacatcctt caggttgcca gcgaccgtcc tctctggtgg ataacgccga
    10381 taacgaacac tccgggcgca gcagaatatt tctcacccag gcaggaagcc ccgccagcga
    10441 caaatggttg agattaccga taatcatgat gcctgcccct taacccactc gggggagatg
    10501 cgtacgtact tgatggcctg acgccaccag gtgtaagcga tatgtagcgt accgtccggg
    10561 ctttgtttga tgctggggta ggagaactca cgatttagct tttccaggga attgttggtc
    10621 atgcagtagc cgtcgccttc atcgagattg cgccgccacg gccagctttt cccaccatcg
    10681 gcagaaatcg ctaccgtcat cggcgcgcgt ggggcccccc agaaagcgga gcgtccgacg
    10741 gctgtcggtt ctttgcggct gtcgtcgcca tcgtcgattt cgtcataaag cgacgcgcga
    10801 cgttctactg cgcctgccgc actcatcgcg ttgtacacca gcgccagttc gccgctggca
    10861 agtgtcgtta cctgaataga cgaattgttg ttgggcaatt cggttgcttg cggtactgac
    10921 cagctctcgc cgttgtccgc ggaatggctg atatagatgt tgtctgccca acggctgcga
    10981 aagagagcaa ccaacgtacc gttgtgcagc attgtgatat tcatatgaac acagccaaga
    11041 ctttgcggca cctcgacatc gcgccaggag tgaccaccgt cagcggaaat tttcactgcg
    11101 ctgatatcat cattgccgac ccatttttcg ccgggttggg tacggcaata aaagacgggc
    11161 agcagccagt tgccgttatc aagcaccgtg attggctgtc gaatgaacgt accgggttta
    11221 tcaagcaggg tggcaatctc gccccaggtt tgtccgaggt cgtctgactt acggtagcgg
    11281 acgatggcgg tgtcctgatt accggatatt tgtgcggtcc ataggagcca cagcacgtta
    11341 tccggcgcga gaaacagcac cgggttttgc tctgaacgcg tgtcatcgtg ggaaagtttg
    11401 accgcatcgc tccactgcat tccgccagcg ggcaatcgcg atccccagac agaaatatcg
    11461 gcaatacctt cctgcgttcc cgcaaaccag acgcacatta acgcgccatc cggcaaaggc
    11521 agaatattcg ccgcgtggtt ttgcgggcag gacgagggca gcatggcggt aagcacatgc
    11581 gaatcgtttt cggatggacg gatgacgccg tcacgaataa gggctacaga catagttaga
    11641 ctccatctta aagttggtaa tagatttatt aatgcgagtg cgattcatgc gtttcgatca
    11701 atgtcgaaac ttttttattt cttctttcac ggataatatt gagtgtctca acacttaatg
    11761 agaaaaatat cgcgaagtag agatactctt tggcaatatg aatgttgaga ctatcggcaa
    11821 ccagcaatac accgacaaac aataaaaagg ttaacgccag tatttttatt gacggcgtgg
    11881 cgttaacgaa atcaccaatt gttttcgccg caaacatcat gacaccgacg gcaatcatta
    11941 cggcggcaat catgataaat atatgctgag aaaggccgac ggcggtgatc accgaatcca
    12001 ggctaaacac gatatcgagc agcattatct gtacaatggc accgcacaac gacagttgcc
    12061 gcgatccgct gccatgttcg tcttcctgcg cgggcgacag ctcttcgcgt atctcctgta
    12121 tcgctttata aatcaggaac agcccgcccc cgagcagaat caacgtgcgg aacgaaatct
    12181 ccagcgtcag cacggtaaaa agcggctggg taatatgtga aagccacgca atggatatca
    12241 gcaataatat ccgcataatc atcgcgcagc ttaaacccag tttacgcgcc atattttgtt
    12301 gtgataacgg aagttttgcc accaccagtg aaagaaaaat aatattatcc accccaagaa
    12361 taatttccag aaaggtgagg gtaattaaag tgagccagat atctggctgc ataaaccaca
    12421 aaaacatggg gtgatccttt attctcttta tctaaacttt atagcgggct ggataataga
    12481 gttaatttaa ttaatgttat aatgacgaat aatttgttga atctcgctga tctgttcagg
    12541 cgtgaaatta accgcgtagc ggctatcacc aacatcatgc cccataagct gcaacgcttt
    12601 tttcaccgca gccggggaga atgctacttt ataaagatcg gtacgcaggc ccgttacact
    12661 ctcctgtgct tcataagacc cttcaatatc actggcatgg aagcgagacc agatggcatt
    12721 gatttctcgc ggcgcgatgt tggcgagccc agagatacag gctgaacatc cctcgacaaa
    12781 ccctttatgg atcagcgaat ccgggccgtt gagcacatta aagccgtcaa tgtcgcgcac
    12841 cgcatcgaga aaacccttca ggctttcgtc gcttccggcg ctgtctttaa tcccaatgat
    12901 attggggtgc gacgccagag cacgcgcggt ttctggctgg atcgtattgc cagtgcgggc
    12961 cgggatgtta tagagaaaca tcggaatgct cagtgcgtcg gcaattgcgc tgtaatgcgc
    13021 aatcaactct tcctgtttca gcggtacgaa gtaaggggca atgaccgaca cggcatctac
    13081 gccaagtttt tcaatctgct gacccagacg aatcgtttcg cgcgtagaga tctcgccaat
    13141 gtgtgccacg accggcgcac gaccagccac ttcatccacg caggtttgcg taacgctgag
    13201 cttctcctgt tcattcaaca caaaaaattc gccgttggtg ccgccgcaaa aaatcgcgtt
    13261 acccgcttcc agttgacgat tcacctgcac ttttaacgcc gggatattca gactgccggt
    13321 cgcggtaaac ggggtgacga tagcggttaa tacaccttca atggttttcg gcatgattta
    13381 ttccttaaat tgatgttgcg gaaacagtgt ttggtaaatg gcctgtacgt cctgtagatc
    13441 aagctggcac ggcacattgt tcataaggcg cttcacgttc agtgccgcct cgctcagaga
    13501 ggcaatatcc tctggcggta cgccgagagc cgcgagattg ttgggtagct tgagttgatt
    13561 gactaatgcc tgtaaccagg tcaccagggc atgagatttt tcttccgcgc tcagggcggt
    13621 ttccgcatcg ggaatgcaat cccagacccg ggcaaatttc tcgaccgccc aggggcgaac
    13681 aaacgccatg cacggcgcca gcaagatggc attcgcgacg ccatgcggca gatgatattt
    13741 gccacctaac gggtaggaga gcgcatgaac gagatgcgtg cccgcatggg ttatcgctac
    13801 gccgccatag taagacgccc acagcatttc cagttttgcg cgcagatcct gaggatcgtt
    13861 cacggcgggt tgaatgtgcc ggaaaagttt acttaaccca gtcagcgccg cgttatcgct
    13921 gaccggattt gccacggtcg cggtaaaaca ctccagtaag tggcaaagag catcaatgcc
    13981 cgtggacgcc gcaatatgcg cgggcatgct ggtggtcagt tccgggaaaa gcgccacata
    14041 gtctggtaac agcacctggg aaataatacc caccttcgtg ctttgctcag ggattgccag
    14101 aatcgcattc ggcgtggctt ctgagccggt tccggcggtt gtaggaatca accatgattg
    14161 cacccgctga gtcggttttt cacccgcaag cagcgcatcc agccccggag attgtggatg
    14221 gcaaagcacc gacaacagtt ttgcgacatc cagcacgctg ccgccgccga cgcccaccac
    14281 cacatcaaaa gacgcgttgc cgagggtcgc catttgctgg cgcacatcat gatgtgtggg
    14341 ctctgcgggc acattatcga taacgctcac ttgcgggcag tgcttttcca gtaaggcgcg
    14401 aatctgctgc acaccgtcga gctgcgcaat attacggtca gtgaccagca gcatttttct
    14461 gatgtcgggt aagagcgacg tcagggaagg gatagcgcct gcgccgctca gtatcgtgct
    14521 attgataatt accacaaata acctctcttc tggtgattga attcaatcat t
//
LOCUS       DADSZD010000110        14502 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.111, whole genome shotgun sequence.
ACCESSION   DADSZD010000110 DADSZD010000000
VERSION     DADSZD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.111"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            160..963
                     /gene="dkgB"
                     /locus_tag="J1Z28_003063"
     CDS             160..963
                     /gene="dkgB"
                     /locus_tag="J1Z28_003063"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBA7717877.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAS
                     VGQAIAESGVPRHELYITTKILIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQYGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(960..1874)
                     /locus_tag="J1Z28_003064"
     CDS             complement(960..1874)
                     /locus_tag="J1Z28_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7717878.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            2115..2915
                     /locus_tag="J1Z28_003065"
     CDS             2115..2915
                     /locus_tag="J1Z28_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBA7717879.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2993..3763
                     /locus_tag="J1Z28_003066"
     CDS             2993..3763
                     /locus_tag="J1Z28_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA7717880.1"
                     /translation="MTIQSHHDHVEKQFSSQAGEYLTSTVHAFGQDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRVLKPGGRLIVMDVMSPGHPVRDVWL
                     QTVEALRDTSHVRNYASGEWLKLINEANLIVDNLITDKLPLEFSSWVARMRAPEALVD
                     AIRIYQQSASTEVKTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(3810..5168)
                     /gene="mltD"
                     /locus_tag="J1Z28_003067"
     CDS             complement(3810..5168)
                     /gene="mltD"
                     /locus_tag="J1Z28_003067"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBA7717881.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5240..5995)
                     /gene="gloB"
                     /locus_tag="J1Z28_003068"
     CDS             complement(5240..5995)
                     /gene="gloB"
                     /locus_tag="J1Z28_003068"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBA7717882.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVKKFPQIVVYGPQETQDKGTTQVVKDSETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            6029..6751
                     /locus_tag="J1Z28_003069"
     CDS             6029..6751
                     /locus_tag="J1Z28_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA7717883.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(6748..7215)
                     /gene="rnhA"
                     /locus_tag="J1Z28_003070"
     CDS             complement(6748..7215)
                     /gene="rnhA"
                     /locus_tag="J1Z28_003070"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBA7717884.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            7280..8011
                     /gene="dnaQ"
                     /locus_tag="J1Z28_003071"
     CDS             7280..8011
                     /gene="dnaQ"
                     /locus_tag="J1Z28_003071"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBA7717885.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNATF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            8144..8220
                     /locus_tag="J1Z28_003072"
     tRNA            8144..8220
                     /locus_tag="J1Z28_003072"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8178..8180,aa:Asp,seq:gtc)
     gene            complement(8352..9398)
                     /locus_tag="J1Z28_003073"
     CDS             complement(8352..9398)
                     /locus_tag="J1Z28_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBA7717886.1"
                     /translation="MDTSLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPETINWAELERDASRLLTRSKDIRILVVLLRCRIQQAGAKGLAEALALLETLCATY
                     PNAIHPQLLATEDITAEEAAVARSNALAALLDHEGVIADIRGITLSNNAAIRLQVRDV
                     ERSLSASRPADALAPESVRQQLADLEARGKLPLDAFRQAAETTERLQRHARETLNDQA
                     PNLSRLTQLLALLPGAIRTTTPEILPQPVAEQPESTTIVHTEEISSEQIALPVHIAEE
                     ISPMSGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRFSEVIN
                     EIPVELLEKWDALE"
     gene            complement(9409..10404)
                     /gene="tssG"
                     /locus_tag="J1Z28_003074"
     CDS             complement(9409..10404)
                     /gene="tssG"
                     /locus_tag="J1Z28_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBA7717887.1"
                     /translation="MSDDFTQAQETPWRYGFLNLMRRVDVQLCTVPAGNTWQPRMEKF
                     RLGQTPALTFAPREIASVSWQEERLHISLYSLGLWGPNGPLPLHYTELARNRTESRRD
                     PTLTRFSDLFHHRWLTQFYQAWRSAQSAGGGLDKPQHDRFAFYIASLSGQDLRESADS
                     PLPDHARLAASAHLVRESRNPDGLATTLAHYFGVPFRIQEYVLHWIAVADDEITRLGM
                     PAPSSVIGNGALIGQAVPDMQYKFRLVIGPLTLEDYRRFLPGGNNLPVLTELVRAFSG
                     FEYCWEIELQLKPHAAPPAVTGGSYQLGWTAWAGKAMHENPVTGMIFEPEHYLAH"
     gene            complement(10401..12284)
                     /gene="tssF"
                     /locus_tag="J1Z28_003075"
     CDS             complement(10401..12284)
                     /gene="tssF"
                     /locus_tag="J1Z28_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBA7717888.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDPEEG
                     DLAKGFTVPRNTAFFSAIPEGESTACQFRSSQNVTLWPLAIEEVRLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLNKLPFYLCGEERTASHLFELLHTSAIAS
                     LAGIPGHFDGALDVNLKQPVMYEGLDPDQGLLPLAWNVFHGHNLLHEYFTCPERFFFF
                     TPTGLSAGLQKINGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVAARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTSYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRPPQPPLAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCNTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPARAGQR
                     GSV"
     gene            complement(12300..12794)
                     /gene="tssE"
                     /locus_tag="J1Z28_003076"
     CDS             complement(12300..12794)
                     /gene="tssE"
                     /locus_tag="J1Z28_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBA7717889.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHDISVSPVQLKEII
                     RRDLSFLLNTVSHEDDIDAARYPDAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     IRFEPRLKASTLQIIPLQDERHQSGHNTLQFEIRGEILTQPYPMAFRVRSALDMEQSR
                     ITFF"
     gene            complement(12791..13621)
                     /locus_tag="J1Z28_003077"
     CDS             complement(12791..13621)
                     /locus_tag="J1Z28_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
                     /protein_id="HBA7717890.1"
                     /translation="MTNNNELPATLSALLRDYSVAEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGYWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPDFIL
                     PPPAWMEDLLTALACNARGEAQEADAHRSRALEAITDTAGQWNGGAFDWISDSDSRTG
                     PVLELIAGGVYIWLPFSQICSLKSPRPAHLTDLIWKPVNVTLNNGDTHSAWLFSRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(13608..>14502)
                     /gene="tagK"
                     /locus_tag="J1Z28_003078"
     CDS             complement(13608..>14502)
                     /gene="tagK"
                     /locus_tag="J1Z28_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
                     /protein_id="HBA7717891.1"
                     /translation="GLQKIQAHGIADNTIYATEGTQIIFTVTSPWIPVLEVNDDVKIA
                     LSLTRHEEAWWIINQSTEYCCTVNDQIVEPHHRMRLNEGDLIEWGLSSWCLVHGNPSS
                     PDLSAVKTTSQTATMSETITEYLDLDWFRQQHLTSQNPFDIIPVHGASSTGEDAQKEN
                     ALTRLYQEYREALLSPEQDLHRHADPFPFNEEPSSQDLDSLRDLVTEAETLQDMVTGG
                     LSIDAILDVLDATGEGETAWPAEKTPPDILHLLSPECAPETAHNTVLPDLTRKEHRII
                     GIDSHYRINPAQHGEISHDKQ"
BASE COUNT         3367 a         3747 c         3838 g         3550 t
ORIGIN      
        1 tattaagaag cctcgagtta acgctcgagg tttttttcgt ctgtatttct attattgcca
       61 aaatcgcaaa aatcctctgc gttttacgcc ctttttccgc aacagtctaa agtccataat
      121 cacctcagtt aacgaaaata gcattaaaag aggcatatta tggctatccc tgcatttggt
      181 ttaggtactt tccgtctgaa agacgacgtt gttatttcat ctgtgaaaac ggcgcttgaa
      241 cttggctatc gcgcaattga taccgcacaa atatatgata acgaagcttc cgtaggtcag
      301 gcgattgcag aaagtggcgt gccacgtcat gaactctaca tcaccactaa aatcttgatt
      361 gaaaatctca gcaaagacaa attgatcccg agtctgaaag agagcctgca aaaattgcgt
      421 accgattatg ttgatctgac gctaatccac tggccgtcac caaacgatga agtctctgtt
      481 gaagagttta tgcaggcgct gctggaagcg aaaaaacaag ggctgacgcg tgagatcggt
      541 atttccaact tcacgatccc attgatggaa aaggcgattg ctgctgttgg cgctgaaaac
      601 atcgctacta accagattga actctctcct tatctgcaaa accgtaaagt ggtcgcctgg
      661 gctaaacagt acggcatcca tattacttcc tatatgacgc tggcgtatgg taaggccctg
      721 aaagatgagg ttattgctcg tatcgcagct aaacacaatg cgactccggc acaagtgatt
      781 ctggcgtggg ctatggggga aggttactca gtaattcctt cttctactaa acgtaaaaac
      841 ctggaaagta atcttaaggc acaaaattta cagcttgatg ccgaagataa aaaagcgatc
      901 gccgcactgg attgcaacga ccgcctggtt agcccggaag gtctggctcc tgaatgggat
      961 taagcctctc tgacagctcc tccgggagct gtttttacat gctcgctaag gaaatcgata
     1021 aaagcccgga tgcgcgtact taccgcacgg tcgctgtaat atacggcact gaatggcatt
     1081 tccactggca atactttatc tgccattaac tccaccaatt ctccgcgagc gatttctctg
     1141 tcgatcatgt agtcggacaa acacgcaatc ccgttgccac tcaggcaaag ctgtttcagt
     1201 gtttccccac tattggatga caaaccgtac ttcacctcat gtaattgtcc atcactacag
     1261 gctatcggcc aggtattgag ggaagcgggt tcagtgaatc ccaggcaaac atgttgcttt
     1321 aaatcgtcga tcgtttctgg cttcccgtag cgggaaatat aatcggggga ggcgataatt
     1381 tttcgataac tgttaaataa cggcctggca cgtaagctgg aatccgttaa cgtaccagcg
     1441 cgtatcgcga catccacttt tctttcgatc aaattaataa tcgtttcgga ggagactagc
     1501 gataaagtga cttccggata gcgttcacgg aaaggcttaa ttaacggcat cagaaagtgc
     1561 agcaccactg gagttgcggc atcgatgcgc aacagtccac gcggtgtatt acgcgtctcc
     1621 ataatttctg attctgccgc tgccatctcc tgcaaaattg actgtacgcg acgaaaataa
     1681 cgctggcctt cttccgtcag gctaagttgc cgcgtggtcc gattaagcag gctaacgcca
     1741 agtttcatct ccagcttttt caccgcccgg cttaccgctg agtttgcttg ccctaattgt
     1801 tccgctgccc ggctaaagct gccgctttct acgaccgaaa caaaaatggc gagttcttcc
     1861 gacgtggctt tcatttttgc tcctgttgca aaatagaaga gatattttga atttatttgt
     1921 cattaaacca tcaggatgcg tgatatgtca tccgatttaa tgttctcaat aatgagcaaa
     1981 attctgaccg gtgtaagcac ttgcttacat aacaatatac aattgctcgt tgaaagagtg
     2041 agctaaaatc cctataacag tagaaccctc ccgagtgcgg aagggttgac gtaatagagg
     2101 tttcaaagtc aaaagtgcga aaaaacacct atgccatgcg ctatgttgcc ggacaacctg
     2161 cggaaaggat cttaccgccg gggtcttttg cgagcatcgg ccaggcatta ccacctggag
     2221 aaccgttaag taccgaagag cgtattcgga tcctggtgtg gaacatatac aaacagcaac
     2281 gcgctgaatg gttgtcggta ttaaagaact acggcaaaga tgcacatctg gtgttattgc
     2341 aggaagcgca gacaacgcca gagttagtgc agtttgcgac cgctaactat cttgccgccg
     2401 atcaggtacc cgctttcgtg ctgccacaac atccttccgg tgtaatgacc ctttcggcgg
     2461 cgcatccagt gtattgctgc ccgttacgtg aacgagaacc cattttgcgt ctggcgaagt
     2521 cggccctggt gacggtctat ccattgcctg acacccgcct gttgatggtg gttaatatac
     2581 acgccgtcaa tttcagtttg ggcgtggatg tctatagtaa gcagttactt cctattggtg
     2641 atcagatagc tcaccacagc ggcccggtca ttatggcagg agatttcaat gcctggagcc
     2701 gtagaaggat gaacgcgtta tatcgctttg cgcgggaaat gtcgctgcgc caggtgcgtt
     2761 ttaccgatga tcagcgccgt cgggcgtttg gccgcccgct tgattttgtt ttctaccgtg
     2821 gtttgaacgt cagcgaagct tctgtactgg ttacgcgcgc ttccgatcac aatccgctac
     2881 tcgttgaatt cagtcccggc aagcctgata aataaggtat gtcaggtctg ccacagggca
     2941 gaccaacgtt tggcgctgcc cttttatact caaacaacaa aaggacagcg caatgacaat
     3001 acagtcccac catgaccacg tagaaaagca gtttagctca caggccggtg agtatttaac
     3061 cagtaccgta catgcattcg gacaagattt acagcgcctg gcggtgcgtc tggctgatta
     3121 tcctgatgca agtgtacttg atatgggctg cggagcaggg catgccagct ttgtcgctgc
     3181 gcaaaacgtg agcacggtgg tggcgtatga cttatctgcc caaatgctgg atgtcgtggc
     3241 acaagctgcc gaagcccggc aactgaaaaa tatcaccacc cgccagggtt atgccgaaag
     3301 tctgccattt gccgataacg catttgatat tgttatcagc cgttattctg cccatcactg
     3361 gcatgatgtt ggtgcagcac tgcgtgaagt gaatcgggta ttgaaacccg gcggtaggct
     3421 gattgtgatg gacgtaatgt ctccgggtca tccagtgcgc gacgtctggt tacagacggt
     3481 agaagcatta cgcgatacct ctcacgtacg aaactacgcc agcggtgagt ggttgaagtt
     3541 aatcaatgaa gccaatctga tagttgataa tttaattaca gataagttac cgctggaatt
     3601 ttcttcatgg gtcgcgagaa tgcgtgcgcc agaagcgtta gtagacgcta ttcgcattta
     3661 ccaacagagc gcatcgacag aggtgaaaac gtattttgcc ttgcagaatg atggctcttt
     3721 caccagtgat atcatcatgg tagaagcaca taaagcagca taaataaaaa aggcaccggg
     3781 ggaatcggtg ccttttatta tctggtttgt caggaatctg gcatgttgtt gtttttcaca
     3841 aacaacgtca gcttatcgcc tggttgcaga ttcgcagtat cgctgttcca gcgcatcaca
     3901 tctttgatgt tcacgccgtg gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca
     3961 cgatacgtaa tgctatcgct gttgtttgcc agtcgctgtg cgctactgcc tgcaccaatc
     4021 gtcaaacttt ggcctggctt cagcttagat ccgcgcagtt tgttccactg ctgcaaatct
     4081 ttggtgctta cgccgagacg tgaagcgata cttgaaagcg tgtcgccaga gcgtacggtg
     4141 taaacacggc tgttaagcgg cgtattgtcg gcaaccagcg tcgactgtac agcagcaatt
     4201 tcgcctgaag ccagagattc acgcagttga tctgcatgct tctttggcac catcacgtac
     4261 tgcggaccac ttgcgcccag cgtggagcct ttcacgcctg cgttgaatgt cttcagcttg
     4321 ctgacggaaa tccccgccat atctgcaacc ttcgccattt caaccgggct gctcaggtgc
     4381 acacgcgcca gagcacggct ttcatcggtc gttggcagac gtacgccata acgcttgctg
     4441 tttttgagaa tatcactcaa tgccagcatt ttaggcacgt actgcttcgt ttcctgcggc
     4501 aacggtaacg accagaagtc cgtggatttc ccacgcgctt tgttcgtttt aattgccttc
     4561 atgacccgac cttcgccgct gttataagcc gctacggtca gaagccagtc gccgtcaaac
     4621 atcttgttca gacgctgcat catgttcagc gcggcagttg ttgaagcaac aacatcgcga
     4681 cgcgcgtcat aattgcgtgt ctgtttcaaa ccataattgc gccccgtgct cggaatgatc
     4741 tgccagatgc ccgcggcatt ggcgccagac gttgcgtgag gatcaaaagc gctctccact
     4801 atgggtagta gtaccagttc cataggcatg ttacgttttt taacttgccc ggctatccag
     4861 tacatatacg gctctgcccg taaagttaca tcgtggagat agctcttatt gcgtaaatat
     4921 ttctgtttct gttcgcgaat ccggtcgttt tccggaattc ccatctttag ctcgtcgcca
     4981 atgaaagccc acaagtcacc atctggcgcg atagacgtcc catcgtccat ccatcgtgcc
     5041 tgacttgtaa actttgctgc ttccccttga ccagctgcag aaaggctctg tgcgtgctgt
     5101 tgaacgttgc cggtactctg gcaacccacg agcaggacag aggcgagtaa tatcgctttt
     5161 gccttcatgt gtgtgtcaat agttgcttaa aagacgaccg atcataacgg cgaacggagc
     5221 cgatgacaag aaagttttat cagaacctat ctttctttga ccttaaccat gcaaaacgtt
     5281 cttcaggttg ttgcaataat gtttcttcat taattacatt aattaaatca atatcttccg
     5341 ttcttaaaaa aacattaatt tgccgctcat ttttcagaat tacgggtagt gttatttgat
     5401 tttttgcccg taactcctta actttacgat aataatcatt tatggacaaa tcgtgcggaa
     5461 gaatactcaa agcaaacttc atatttgata aggtatattc atgagcacaa cataccaatg
     5521 tatcgtcagg taacgcactt aactttttaa gtgattgata catttgtgat ggtgtccctt
     5581 caaacaaccg accacaccca ccagaaaaca gcgtgtcgcc gcaaaataga taaggtttac
     5641 tgaagtaaca gatatgtcct aaagtgtgac ccggtgtggc aattacacta aattcatgcc
     5701 ccaaaacgaa ggcagtttcg ctatctttga ctacctgtgt tgttccctta tcttgtgtct
     5761 cttgtggacc atacaccaca atttgcggaa actttttcac cagttctttt acgccgccaa
     5821 cgtgatcgtg gtgatggtgg gtgagaaata tggcctccgg ttgccagtta ttggcggcaa
     5881 tggcgtttaa tactggctct gcgtctccgg gatcgacaat caggcagcga cctgcttcat
     5941 cattcaaaac ccagatgtaa ttgtcatcaa aggcgggaat actgttaaga ttcatagatt
     6001 acctctcagt gtgaaacgga aggttgtgat gaaaccggca agagtccctc aaactgtcgt
     6061 ggctcctgat tgctggggcg atttgccctg gggagagctt tatcgcaagg cgctggagcg
     6121 tcagctcaac ccgtggttca ctaaaatgta tggttttcat ctgcttaaga ttggcaattt
     6181 aagcgcagaa atcaattgcg aagcgtgcgc ggtttctcat caagtgaatg tttctgcgca
     6241 aggaatgccc gtccaggtgc aggcggatcc acttcatctt ccttttgccg ataaatccgt
     6301 tgatgtttgt ctactggcac atacattgcc gtggtgcact gatccgcatc gtttattgcg
     6361 tgaagccgat cgggtactga ttgatgatgg ctggctggtg attagtggct tcaatcccat
     6421 cagtttaatg ggattacgca aacttgtgcc ggtattgcgc aaaacatcgc cctataacag
     6481 ccggatgttc actctgatgc ggcagctgga ctggctctct ttgttgaatt ttgaagtgct
     6541 acacgccagc cgtttccacg ttcttccgtg gaacaaacac ggaggaaaac tattgaatgc
     6601 gcatattcct gcgcttggtt gcttacaact tattgttgcc cggaaacgga ctattccttt
     6661 aacgctaaat ccgatgaaac agagcaagaa taaaccgcga attcgccagg cggttggtgc
     6721 cacccggcaa tgtcgtaaac cacaggctta aacttcgact tgatagcctg tatcttccag
     6781 tgtgggattc attgccgcag cacgagccag ttcatcacag cgttcgtttt ccgggtgtcc
     6841 ggcatggcct ttaacccatt cccatttgat ttgatgctgc cccagagcag catcaagacg
     6901 ttgccagaga tcgacatttt ttactggttt tttgtctgct gttttccaac cacgtttttt
     6961 ccagttatgg atccactggg tgataccctg gcggacatac tggctgtcgg tactcaaaat
     7021 gacttcgcaa tgttctttta acgcctccag cgcgacaata gcggccatca actccatacg
     7081 gttgttggtg gtgcgggtgt agccagcgct aaaggttttc tcgtgtccgc gatagcgtaa
     7141 aatagcgccg taacccccag gtccaggatt acccagacac gaaccatcgg tgaaaatttc
     7201 tacctgttta agcatctctg gtagacttcc tgtaattgaa tcgaactgta aaacgacaag
     7261 tctgacataa atgaccgcta tgagcactgc aattacacgc cagatcgttc tcgataccga
     7321 aaccaccggt atgaaccaga ttggtgcgca ctatgaaggc cacaagatca ttgagattgg
     7381 tgccgttgaa gtggtgaacc gtcgcctgac gggcaataac ttccatgttt atctcaaacc
     7441 cgatcggctg gtggatccgg aagcctttgg cgtacatggt attgccgatg aatttttgct
     7501 cgataagccc acgtttgccg aggtagccga tgagttcatg gactatatcc gcggcgcgga
     7561 gctggtgatc cataacgcaa cgttcgatat cggctttatg gactacgagt tttcgttgct
     7621 taagcgcgat attccgaaga ccaatacctt ctgtaaggtc accgatagcc ttgcggtggc
     7681 gaggaaaatg tttccaggta aacgcaacag cctcgatgcg ttatgtgccc gctacgaaat
     7741 agataacagt aaacgaacgc tgcatggggc attactcgat gcccagatcc ttgcggaagt
     7801 ttatctggcg atgaccggtg gtcaaacgtc gatggctttt gcgatggaag gagagacaca
     7861 acagcaacaa ggtgaaacaa caattcagcg cattgtgcgt caggcaagta agttacgcgt
     7921 tgtttttgcg acagatgaag agcttgcggc acatgaagcc cgtctcgatc tggtgcagaa
     7981 aaagggcgga agctgcctct ggcgggcata aatacctgta aaaggcgcta aaattagcga
     8041 cttgggcgat ttttgcagca aacgattcaa aagatgagaa aaaccgttga cgaagggtga
     8101 ggcaatccgt aatattcgcc tcgttcccaa cggaacacaa cgcggagcgg tagttcagtc
     8161 ggttagaata cctgcctgtc acgcaggggg tcgcgggttc gagtcccgtc cgttccgcca
     8221 atattcagaa agcccgaatc attactgatt cgggctttcg tcgttttggt gaagcgagaa
     8281 tttttcactc cgcaagtaaa acgaatatga tttaaaaaat ggccagataa tataaattct
     8341 ggccacttct gttactccag cgcatcccat ttttccagca gttctactgg aatttcattt
     8401 attacctccg agaagcgttt tccgaccagg cgttctgcct ggcgcagcaa aggaatggtc
     8461 gggctgctcg gttcgctgtg ctcaaaccag cgacggatga cacgcaggcg ttccagcgcg
     8521 tcgttccggt cgcggatctg ccggggttct gcgccggaca tcggtgatat ttcctccgcg
     8581 atatgcaccg gtagcgcaat ctgctctgat gatatctctt cagtatgaac gatggtggtg
     8641 ctctccggct gttcagccac tggttgtggc aggatctccg gcgtggtggt ccgtatcgct
     8701 cccggcaaca gggccagcaa ttgtgtcaga cgggataggt tcggtgcctg gtcattcagc
     8761 gtttcacgcg catggcgttg cagccgctca gtggtctctg cggcctggcg aaaggcatcc
     8821 agcggcagtt tgccgcgcgc ctccaggtcg gccagttgct ggcgtaccga ttctggtgcc
     8881 agcgcatccg ccgggcgtga tgcagacagc gaacgctcca catcccgtac ctgaaggcgt
     8941 atggcagcat tgtttgacag tgtaatgcca cgaatatccg ctattacgcc ttcatggtcc
     9001 agcaacgccg ccagggcgtt actacgtgcc actgccgcct cttcagcggt gatatcttca
     9061 gtcgcgagca gttgaggatg gatggcgttc gggtacgtgg cgcacaacgt ttccagcagc
     9121 gcgagcgcct ccgccagtcc cttggcaccg gcctgctgga tacggcagcg cagcaggaca
     9181 accagaatgc ggatatcttt gctgcgcgtc agcaggcggc tggcatcccg ctcaagctcc
     9241 gcccagttaa tggtttccgg cgtactgaca aaatcgccgt attgcgcttc ttcacgcggt
     9301 gcggcgcgcg tgaacagtag caggtattcc ggatcgtact ccagatccgg gccgcagggg
     9361 cattctgccg aaattgggtc agacagggat gtatccatta aattaacctc agtgcgccag
     9421 gtagtgttct ggttcgaaaa tcatgcccgt caccgggttt tcgtgcatgg ctttacccgc
     9481 ccatgcggtc cagcccagtt ggtacgaacc accggtaaca gccggagggg cggcgtgggg
     9541 ctttagctgt agctcaattt cccagcagta ttcaaaaccg ctaaaggcgc ggaccagttc
     9601 tgtcaggact ggcaggttat tgccgccagg cagaaaacgt cggtaatcct ccagcgtcag
     9661 cgggccgata accagccgga acttatactg catgtccggc acggcctggc cgatgagtgc
     9721 accgttgcca ataacggaag agggggctgg catccccagc cgggtgatct catcgtccgc
     9781 cacggctatc cagtgcagga catactcctg tatccggaaa ggtacaccga aatagtgcgc
     9841 cagcgtggtg gccagaccgt ccggattgcg ggactcacgc accagatggg ccgatgccgc
     9901 caggcgagcg tgatccggca acgggctgtc ggcgctctcc cgcaaatcct gcccgctgag
     9961 gctggcgata taaaaggcaa aacggtcatg ttgtggttta tccagaccgc ccccagcgga
    10021 ctgggcgctg cgccatgcct ggtaaaactg cgtcagccag cggtgatgaa aaagatcaga
    10081 aaaacgggtt agcgtcgggt ctcttcggct ttcagtccgg ttgcgcgcca gttcggtgta
    10141 gtgcagcggc agtggaccgt tcggccccca cagcccgaga ctgtacaaac tgatgtgcag
    10201 ccgctcttcc tgccagctca cactggcgat ttcccgtggc gcaaaggtca gcgcgggagt
    10261 ttgtccgagg cgaaattttt ccatccgtgg ttgccaggtg ttgcctgccg gaacagtaca
    10321 caactgcaca tccacgcggc gcatcaggtt cagaaaaccg taacgccagg gggtttcctg
    10381 cgcctgagtg aaatcgtcgc tcatacgctt ccccgctgtc ccgccctggc aggccaggtc
    10441 ataattttgc cgcgctgcat gctgtgcagc gtcatctgcg agaaggtatt aatggaaaca
    10501 tgacgggcga tatagtgctc caacaccaga ccgaacagat acggactgat accggagaac
    10561 ccctcctcat ccacggtcag ttcacaactg acgccgcgcc catagaccag cagcccggaa
    10621 ccgggcagac ggcgggtgac cggggtggtg ttgcagccaa tcaggctgcg tacctggcgc
    10681 gactgcggac tgtcatgcgc cggaataaac aggttaagca gatcgcgcag cgcctgacca
    10741 ccggtacggt gatccagatc ggccagcggc agatagttaa aggataactg gcggatcaac
    10801 cgccaggcca tttcacgttc agccagcgga ggctgcggcg gacgcggcgg acggataagc
    10861 cccacgcccg ccaccgggat ggccgcatcc acggtcagat cgtcccgtcc attgcgggca
    10921 ataagacagg gcagatcgcg gttggtgacc atagccgtga tggtgatatg gcgcagattt
    10981 tccgggtacg gcgcttcata ctggtcaacc agcgacagga acacttctga tcctgtatag
    11041 gaggtgcggg tgccatagcg gcgggcattt tccgacaacc ggcgcggctc ccggcgcagg
    11101 gaaaaataac gtccgtgatt gccttcatca ttattgcggg tgtggtagag cgggcgaaag
    11161 gccatcttcc gtgtggtgtc agtttccagc ccttccactt cctggaccga gaacacctca
    11221 taatccagcg gatgggttct gtccaccacc agatgctgtt ccgtcgtgct gtgcgtgaca
    11281 tcgatccggg cggcgacgcg gggaaacagg ttgataaccg gggtacagaa caggctgaac
    11341 tgtgccgcgt ttgtctggtg gatcagccag tccggcggca ggcggttcag caggataaca
    11401 atctccgcca cgccgccgtt aattttctgt aacccggcag acaggcctgt cggggtgaaa
    11461 aaaaagaagc gttccggaca ggtaaaatat tcatgtagca ggttatggcc gtgaaacaca
    11521 ttccacgcca gcggcaacag tccctgatcc ggatccaggc cttcgtacat aacgggctgt
    11581 ttcagattca catccagtgc gccgtcaaaa tgccccggaa tgccagccag gctcgcgatg
    11641 gcgctggtat gcagtagttc gaacaggtgt gaggccgtgc gctcctcgcc gcagagataa
    11701 aatggcagct tgttcagccc tgcaagctga ctgaacgtca gttcaccaaa ggtgcgcagg
    11761 gtaatacgca gcgcaccagc gacgtgaatg ttggctggca ggtagcggtg cagggcaggc
    11821 atatccggcg gcgcggcggt cagccgcacc tcttcgatgg caagcggcca cagcgtcaca
    11881 ttctggctgc tacgaaactg gcaggcggta ctttcgcctt ccggaatggc ggaaaaaaag
    11941 gccgtgttgc gcgggacggt gaatcccttc gccagatcgc cttcttccgg atcgggatga
    12001 agctgcgcca cgctcattga gggcgtgggt gtgacgtaat tggggctgac cacttccagc
    12061 agacgctggg tgaaacgggg gaattcagcg tcaattttta gctgcgtgcg ggcggtcagg
    12121 aagctgaagg cctcgaccat tcgctccaca tacggatcgg caatatccgt tccctgcata
    12181 cccagtcggg cggcgacttt aggatgacgg gcggcaaatt cggctcccgt ttcccgcaga
    12241 tagctcagtt cgcggttgta gtattccagt agccgtggat ccatcggttt ctctctgaat
    12301 taaaaaaacg taatgcggct ctgttccata tccagcgcgc tgcgtacccg gaatgccatc
    12361 gggtagggct gcgtgaggat ctcgccgcga atttcaaact gtaatgtgtt atggccgctc
    12421 tggtggcgct catcctgaag tgggatgatt tgcagggtgg acgctttcag ccggggttca
    12481 aaccggatga tggtacgcaa aatggccctg cgaatatcgt cccacttgtg ttcatacaga
    12541 aagctgcccg ccagtggcgg caggccgtag ttcagtacgg acgctgccgc gtccggataa
    12601 cgtgcagcat caatatcatc ttcatggctg acggtgttga gcagaaatga caaatcccgg
    12661 cgaatgatct ccttaagctg aaccgggctg acgctgatat cgtggtcgcg tttctggttt
    12721 ggggcatcat cacacaggcg gtcaaacagc gtgggcagca ggtgattgcc cggacgctgg
    12781 cgggaagcgc tcacgcgtcg cttccttcag cggtgttaaa ggtacaggtg gacaaatcca
    12841 gcaggctgat atcgccgtga ccggtgagcc acaccctctg acccagcgcc tgtaccagag
    12901 tctctcccgg cccgtcctgc cagaccgttt cccggcacag acgcagggag tcggcagcct
    12961 tttcagagcc gctgtagcgg gaaaacagcc aggcactgtg tgtgtcgcca ttattcagtg
    13021 tgacgttgac gggtttccag atcaaatcgg tcagatgcgc aggccgcggc gattttagcg
    13081 aacagatttg tgaaaacggc agccagatat atactccgcc cgcgatgagt tcgagtaccg
    13141 gaccggtacg ggagtcgctg tcgctaatcc agtcaaatgc gccgccattc cactgccctg
    13201 cagtatctgt tatggcctca agtgcgcgac tgcggtgggc gtcagcttcc tgcgcttcac
    13261 cacgagcgtt acaggcgagg gcggtcagca gatcctccat ccaggcgggc ggcgggagaa
    13321 taaaatcggg ccgctgttct ccctgaaagc aggcgtggcg gtatatctca cagcgtatca
    13381 gctcgccaaa tacctgcgct tcgcgggtat aatttgcgtc catccgggcg catagctgga
    13441 tctgttgcag tgcccgcgac cagtagcctg ccacgcacaa taactgaaat aggctgtggc
    13501 ggcgggaggc ctgcgccggg tgcatcctga cctgctgctc tgccatttga atgccctccg
    13561 ccacggaata atcccgcagc agcgccgaca gggtagccgg tagctcgtta ttgtttgtca
    13621 tgagatatct ctccgtgttg tgccgggttt atccgataat ggctgtcaat gccaatgatg
    13681 cgatgctctt tgcgtgtcag gtcgggcaga acggtgttat gcgctgtttc cggtgcgcat
    13741 tccggggata gcaggtgcag gatatctggg ggcgtctttt cggcaggcca ggcggtttcc
    13801 ccttcgccgg tggcgtccag gacatcgaga atagcgtcaa tgcttaaccc gccagtgacc
    13861 atatcctgca atgtctcggc ttcagtgacc agatcacgca atgaatccag atcctgtgag
    13921 gagggctctt cgttaaaagg aaaaggatca gcatggcgat gaagatcttg ttcaggagat
    13981 aacaacgcct cgcggtattc ctgataaagt cgagtcaggg cattttcttt ttgagcatct
    14041 tctccagtgg aggaggctcc atgaacagga atgatatcga atggattttg tgatgtcaga
    14101 tgctgctgcc tgaaccaatc cagatccaga tattcggtta ttgtttccga catggtggca
    14161 gtctgcgatg tcgtttttac tgcgctcagg tccggagatg atggattgcc gtgcacaagg
    14221 caccatgaag ataaccccca ttctatcagg tcgccttcat tcagacgcat acggtgatga
    14281 ggttccacaa tctggtcgtt taccgtacag caatattcgg tagactgatt gataatccac
    14341 catgcttctt cgtggcgggt cagactgagg gcaatcttta catcatcatt cacctccaaa
    14401 acaggaatcc agggtgaggt aacggtgaag ataatttggg ttccttcagt cgcgtaaatt
    14461 gtgttatcgg cgatcccgtg cgcctgtatt ttttggagtc cc
//
LOCUS       DADSZD010000111        14243 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.112, whole genome shotgun sequence.
ACCESSION   DADSZD010000111 DADSZD010000000
VERSION     DADSZD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.112"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(97..462)
                     /locus_tag="J1Z28_003079"
     CDS             complement(97..462)
                     /locus_tag="J1Z28_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717892.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCTLSMM
                     MAEGIVIMKYELWYIPPALLGLAGVLSWLLLLLHTGYFSGISEGVHKRLWLAVAAVFS
                     GAGMIIFYWLLHASENIIK"
     gene            complement(476..1216)
                     /gene="traF"
                     /locus_tag="J1Z28_003080"
     CDS             complement(476..1216)
                     /gene="traF"
                     /locus_tag="J1Z28_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBA7717893.1"
                     /translation="MNKALLPLLLCFIFPASGKDAGWQWYNEKINPKEKENKPVPAVP
                     RQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKAM
                     LAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAITKLAEHYGIMFFYRGQDPIDGQLA
                     QVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGSV
                     RPLSYGFISLDDLAKQFLNVSEDFKPNF"
     gene            complement(1209..1466)
                     /gene="trbE"
                     /locus_tag="J1Z28_003081"
     CDS             complement(1209..1466)
                     /gene="trbE"
                     /locus_tag="J1Z28_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBA7717894.1"
                     /translation="MKVIFTSNRFIDFIIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERESDERNEDE"
     gene            complement(1505..3301)
                     /gene="traN"
                     /locus_tag="J1Z28_003082"
     CDS             complement(1505..3301)
                     /gene="traN"
                     /locus_tag="J1Z28_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBA7717895.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEITRNEYTNYICERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRIYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTESGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEDACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALSKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFDRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLK"
     gene            complement(3298..3936)
                     /gene="trbC"
                     /locus_tag="J1Z28_003083"
     CDS             complement(3298..3936)
                     /gene="trbC"
                     /locus_tag="J1Z28_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBA7717896.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGYSGK"
     gene            complement(3945..4937)
                     /gene="traU"
                     /locus_tag="J1Z28_003084"
     CDS             complement(3945..4937)
                     /gene="traU"
                     /locus_tag="J1Z28_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBA7717897.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(4934..5566)
                     /gene="traW"
                     /locus_tag="J1Z28_003085"
     CDS             complement(4934..5566)
                     /gene="traW"
                     /locus_tag="J1Z28_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBA7717898.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVSGIGRTEKYGNRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEQGRK"
     gene            complement(5563..5949)
                     /gene="trbI"
                     /locus_tag="J1Z28_003086"
     CDS             complement(5563..5949)
                     /gene="trbI"
                     /locus_tag="J1Z28_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBA7717899.1"
                     /translation="MSSTQKPADVTAERRIHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(5946..8573)
                     /gene="traC"
                     /locus_tag="J1Z28_003087"
     CDS             complement(5946..8573)
                     /gene="traC"
                     /locus_tag="J1Z28_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBA7717900.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(8733..8945)
                     /gene="traR"
                     /locus_tag="J1Z28_003088"
     CDS             complement(8733..8945)
                     /gene="traR"
                     /locus_tag="J1Z28_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBA7717901.1"
                     /translation="MSDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPIPEA
                     WRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(9080..9595)
                     /gene="traV"
                     /locus_tag="J1Z28_003089"
     CDS             complement(9080..9595)
                     /gene="traV"
                     /locus_tag="J1Z28_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBA7717902.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMVQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(9592..9842)
                     /locus_tag="J1Z28_003090"
                     /pseudo
     CDS             complement(9592..9842)
                     /locus_tag="J1Z28_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
     gene            complement(9854..10084)
                     /locus_tag="J1Z28_003091"
     CDS             complement(9854..10084)
                     /locus_tag="J1Z28_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBA7717903.1"
                     /translation="MTEQTETTEIKMNMRNINVITALSVPGKTVSDDFMHAVLSNCAT
                     RIVLPAPEKFGSESLPDNFNMTAVGVMKNSEI"
     gene            complement(10038..10628)
                     /gene="traP"
                     /locus_tag="J1Z28_003092"
     CDS             complement(10038..10628)
                     /gene="traP"
                     /locus_tag="J1Z28_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBA7717904.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(10618..12045)
                     /locus_tag="J1Z28_003093"
     CDS             complement(10618..12045)
                     /locus_tag="J1Z28_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBA7717905.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPLPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGGVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(12045..12749)
                     /gene="traK"
                     /locus_tag="J1Z28_003094"
     CDS             complement(12045..12749)
                     /gene="traK"
                     /locus_tag="J1Z28_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBA7717906.1"
                     /translation="MFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAISNTSPNLFT
                     VPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLSIQAVPREG
                     AGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQVPVTKETLQ
                     APAGLSSVADAVWTGNHLKMVRFAVENKMLSALNIRESDFWQPGTRAVMFSQPASQLL
                     AGARMDVYVIRDGEGN"
     gene            complement(12760..13326)
                     /gene="traE"
                     /locus_tag="J1Z28_003095"
     CDS             complement(12760..13326)
                     /gene="traE"
                     /locus_tag="J1Z28_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBA7717907.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNVIQGVNNYRLQNE
                     QRTAVTPMAFNAPFAVSQNSADASYLQQMTLSFIALRLNVSSETVDASHQALLQYIRP
                     GAQNQMKVILAEEAKRIKNDNVNSAFFQTSVRVWPQYGRVEIRGVLKTWIGDSKPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(13348..13659)
                     /gene="traL"
                     /locus_tag="J1Z28_003096"
     CDS             complement(13348..13659)
                     /gene="traL"
                     /locus_tag="J1Z28_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBA7717908.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(13674..14039)
                     /gene="traA"
                     /locus_tag="J1Z28_003097"
     CDS             complement(13674..14039)
                     /gene="traA"
                     /locus_tag="J1Z28_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001668211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBA7717909.1"
                     /translation="MDAVLSVQGASAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(14073..>14243)
                     /locus_tag="J1Z28_003098"
     CDS             complement(14073..>14243)
                     /locus_tag="J1Z28_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="HBA7717910.1"
                     /translation="EDTNNRLIKAKDRSGRSKTIEVQIRLRDHLKRFPDFYNEEIFRE
                     VTEESESTFKEL"
BASE COUNT         3268 a         3995 c         3453 g         3527 t
ORIGIN      
        1 aataaattac ttgtccgcgt tacaaataat aagagcaaaa ctcgcactta taatatctat
       61 tctctggatg tgtcttattc tcgcattaac tattcgttat tttattatgt tttctgatgc
      121 atggagcaac cagtagaata taatcattcc cgcaccagaa aacactgcgg caacagcgag
      181 ccacaaacgc ttatgcactc cttctgatat gcctgaaaaa tatcctgtat ggagtagcag
      241 cagcaaccat gacaacactc ctgccagacc cagcagggca ggagggatat accacagttc
      301 atatttcata ataacaatac cttctgccat catcatggac agggtgcaaa caacaaaaca
      361 gtcccatgaa aaaaaacgcc tgtcagcagc aaggaaaatc actgtcagtg acatgactgt
      421 cacctgggcc agcaataaag gccataaaga aataaaattc atttaaacga cctctttaaa
      481 aattcggttt aaagtcttca gaaacgttca ggaattgttt tgccaggtca tccagcgaaa
      541 taaagccgta tgataacggg cgaacactgc cctgtttcgg gtcaaccagc atcatggccg
      601 ggaaatattt cacgccgaga cgctgcgcct gcccttggtc agtccgggaa tccggcaata
      661 gtggattaat cacgccatcc acggaaacag gaataacact cagaccatac gtatcccgga
      721 agccattaat gacctgcgcc agctgcccgt cgatggggtc ctgcccccgg taaaaaaaca
      781 tgatgccgta gtgttcagcc agtttcgtaa tagcctgtcg ctgctgcgcc tggtctgctg
      841 ccagctggtt ccgtaccgtg ccgttgtaat ggctgtactg caggttatag tccagttcag
      901 gatgtgccag catggccttt ttggcgctca tggtgaaaag cccggcctgc tgagtccagt
      961 aattctgcag ccggaaatat ttcacaaaat tatccacgcc gggatacaga atggcttcgt
     1021 acagcgcacg ctttgttgcc gtctgcagtg cggccagttt ctgcataata tccggctcct
     1081 gacgtgggac tgcaggtaca ggtttatttt ctttttcctt tggatttatt ttctcgttat
     1141 accactgcca gcctgcatct ttccctgacg ccggaaaaat aaagcagagt aacagtggca
     1201 gtaatgcttt attcatcttc attcctttca tcactttctc tttcatcagg tttctgcagg
     1261 tcagtcaggg cagaaaaaca aaaatacaga ataaacagga gcacaccgga gtaaaggata
     1321 acgaaagcag cggcaagcat cccgtctgcc gtggtttctt ttaccgtatc ccacgaccag
     1381 ataattaccg gagaaatgac aatcgctgta atcaaaagcc ggataataaa atcaataaaa
     1441 cggttcgacg tgaatatgac tttcatcatt ttccccggtg attttccata ccttactgtc
     1501 ctgcctattt cagctggtca gcaatctgtt ctttcacttt ctgcgtcaga acgccactgt
     1561 cagggatttt ctggttattc atcaggtctt cgtagaagtt agtgaaatcc agtctgtcga
     1621 actgaatttt ctgcagttca tcaaccgtaa ttccccgaca gtcaggatgc ttcgcactac
     1681 caaaactgat acgtagctgc ccgttgcgcc cctgctgctg gactatctgc gccagtttcg
     1741 aatcaaactg acagtagctg cgttttttct ccagacagac acccagcact ttcttcgaac
     1801 agaattcccc gacgctgacg gtcagtttgt ttgattttgc tttagacagg gctttttcat
     1861 cactgctgca tttggccagc ccaatatcct gtccccagcc gctgtcctta cagcagttgc
     1921 tgtatccggc tgcagccttc ttacaaaatt tcgcctgtcc ggtgaaagca cggacatcca
     1981 ccccgttcag cgccgcgaca tccttacctg cagcggccag tgccgccagc tgagaaacgg
     2041 cttcggcaaa atcattactc tgcccgctct gagccttatc gcattcgcca tcgagacaga
     2101 agacgtctcc cccacagacc atcactttcc cggatgtttt tgactcgcag gagtacgtgg
     2161 catattcatg cagacaggca tcttcttcgg agtaaaaggc gcactggtgc gacaccaggg
     2221 tacaggcagg attgtcggtg tacgtctgac aggttccctt gtctgctgac tgcgtgacat
     2281 acgtgtcccg gtatgcccag cagctctggt acatgctgta tgactgaccg tctttcacca
     2341 gcgtcctgtt cccgccagac tccgtacatt ctgtttttgt cagtttcccc aactccttgc
     2401 tgaacgggca gctttcagac cagacaacat acggggtgta cgaggctttt tttaccctga
     2461 caaccagacg cagcttcatt gccgtatgac gtttgaattt tgtattcccg acccggacgg
     2521 ggtgagatga cgtaaaagca acaccggcag tcagtgtttt ctgtactggt gtaaatgagg
     2581 catctgctga gtatttggca ttccagctca gtggggttcc cagtactgta attgtcatag
     2641 gtacactctt ctgcaggtaa atacgactcc agctgtaatg tgcatcgaca atttcgccgg
     2701 taacgggaga ggtaatggag acaacatact gaccattcac ttcacgtgca ggtagctgac
     2761 tcatttcata ctccagcgtc cgggtttccc atgtggtgct cccctgcagc tccatacggg
     2821 cagttcgtgt gcagtactgc tccacctgta aatcccgctc acagatgtaa ttcgtgtatt
     2881 cattccgggt aatctcctgc gcactgcact gctgcccggt gtttcccaca atgctgtcag
     2941 cccggttcac cacatccctg ccggtctgga taaacggtgc atccggtgaa agaatgtctt
     3001 tcggcttgtt catgaatgac tctgtgatgg tttttccggt ttcaccggtc gcccattccg
     3061 tggtgccgtc atttttcagg ccgccatccc cgccggcagt cacgccaccg taatattttg
     3121 tctcgtccgg attcgcgtta tagccgggga tactctcctg tggcttaaag ccctgaatac
     3181 tgctgcttcc ctgccctttg atctggtgag caaaatcaga accggcccgg taatcactgt
     3241 tgctgtcagc ctgtgccatg ccggcaacca gagccagtat cagaggtaaa atacgtttca
     3301 tttcccggaa tatcctttcc ctgccagtaa atcatgagcc acctgccggc agtcacccgt
     3361 ggcggccact ttctccagcg cctggccgac ccgcaggttt ccccggataa tgtcgtatcc
     3421 ctgactgcag aacaccacca gtgccggtac actgcgaatg ccgtactgtg aaaacagcgt
     3481 cgggtcgatc tgaacaccat cagttgcgcc gtctttcacc agggacaaca cggcttcagc
     3541 ggtggtcttc aggtcattat tcaccatgcc ccgcagtgtg gccggaatac cgtagtgccg
     3601 ggtttcgccc agcatccgtt tcagcccctc ttcgggaatg gaaaacgaca caaaatacag
     3661 ggcaccctgc cggggtttcc cgtcctgcga agcctgctgt ttacgaacca gctcatccag
     3721 gaaatggtta tctgaacgct gaagggggtt ttccagcacc tgtttctccg cccaggcttt
     3781 cagctgatgg tcaggttttt cacgcagttg tctgcttaaa ttttcctgct gcttcaggaa
     3841 ctggcggttt tcaggagtgt tcacgttttc tgacgccata accgccccgt tcagcatcat
     3901 cagcagtgct gccagagatt tcatactcag cttcatcatt cacctcacag gaatacacag
     3961 ttacgtttac gccacatcag gtagccgaag tttttctttg tgttgggcgg atttttcccg
     4021 gtttcccagc gggtcacgct gcgcccgaac gggtggcact gtccgctgtc cgggtacata
     4081 ttcaccatct ggtaacgcca gcgttctttg ggcaggattg gggacggata ttcattacag
     4141 acggcgttat ttttcccgat ggtttccata atcatgccct gacggtgcag cttgaacgcc
     4201 atgcgttcac tgaccagcag ggaggactgc aacggactgg actcattact cacccagcca
     4261 ttgaacgggt acatactgcc ctgcgaacca gcacaccaga acagaacatc gagaggcata
     4321 ttaaaggcgc tggcaatggc atctgctgca caggccccct gtgctatcgg attggcaaag
     4381 atgacagctt ccggattgag aatggtggtc aggctgctgt ccgtccaggt ggggtcaatt
     4441 tcagaaagat aagcgatatc caggtcacca ccttccagac agcccagcga tgtgatgatg
     4501 ttcagccagt acgtcagcgg gtatttgtac cagtgaacgt gatagaacgc cccgttcacc
     4561 tgcttctcat cttttttcgc cgttccctgt gccgttttac caaaagccgg caggctgaag
     4621 cccaggttaa ccatgcatcc cggtgaccgg gtgacgtccg tcagcgccat cggctcccag
     4681 taaccaatgg ccagcccgat acgcctgaac agcggcggcg gtgccggaca aatctgaatg
     4741 ggcatcgacg ggttcgccgt gtcggggacc ttaccctgac tgactttgat actgcccagc
     4801 gagagcggga aaatacagct ccagcagata tccgtgatcg ggtttacaaa acgcccctca
     4861 caggcagaat ccgcagacgc tgcagggaca tgaccggcga gaaggaataa catcagcagc
     4921 cacaggcttc gcttcatttt ctgccctgct ctgccggaat aaattccacc ttcaggaagc
     4981 gatcgccggg aacggcgctc acccgtgccg ggacctgatc aatgccaagg cgctggcaga
     5041 gcacaccgtt ctggtcaaag taaacacggc tgtccagaga cttctgcatc tccgggatac
     5101 tgccctgcac gaggataatt ttgctctcca gtgtgggcgg cgtctggcgt ttcatccagg
     5161 ccacctgcgc cggatcatcg ccattgatga aatacagcgt ctggttaaaa ggaacatact
     5221 gcagcggatt catcacttca ccctgacggg caaacacccg cccttcgtta tcccggatat
     5281 ccgcagccag tctgacggac gggtcaaaca accggttgcc gtatttctcc gtgcgtccga
     5341 taccggacac ggcagggggc cgcagactgt tgcggatcac ccgctcctta aaggcatcca
     5401 ttttccggcc catctcaccg gactgctcca gtgccgtcag gcgctgcatg atcaccgtca
     5461 gcatgtccgg ctcctttacc ggccacagat caccccaggt accaagatcg gcggcggcca
     5521 cactctgccc ccatatcagc agggcaatca gcccccggca tctcatggtt ccgccctcat
     5581 tcgccgggca atatcctgct ggatttcccg ggtgatatcc ggtgccccct gtaccacagc
     5641 aggcgacacc agaatgaccg cgtggtgttt ctgctgccac gcctgcaggc tggcttccag
     5701 tgccgtgtta aaacgcgctg aaagggcttt tgactgcgct tctgacagtt gtttctgact
     5761 ggcgctgtca aaaaacgcat ccacggtctg tttcatgttg aaggcgacag tcaccggtgc
     5821 gttcagtctg acaatgccgt aactgacggc tgcgttcagt aacaccattg ccaggcatcc
     5881 cggcaccgtc caccaccagt ggatgcgtcg ttctgccgtg acgtcggcgg gtttctgcgt
     5941 tgaactcatg ccacgctcct gtatttctca tgttcttcca gccaggcttc cagcgaagcc
     6001 atctccggcc ctgacttctt ccacgccagc tgccataccg cctcatgaat actcagcccc
     6061 tctttacgct tctgctgcac aaactcaaaa tccgggccgt cagagctgta catggcgcgg
     6121 cttaacgggt ccacaaacag acggtgccag gaggaatggt tttccacctg cagcaggaag
     6181 gaactgaacc actggtcttt ggccgcacca aacttaccaa tcatgtcgcg ctgcagtggc
     6241 aggaactggt ccggatacag ttggttgtat ttcgcgaact ccttcgcact ctgtttgagg
     6301 ataattttgt aggaggagtt accccatgcc gcgcgggcag cactggaggc cttgtcagag
     6361 tcaaagtcga cgatgttctg tgtgatggtg atataggcac cggtatgacg gcgggccgta
     6421 cggtagcctt tctcaataaa ttcaccgacc ttgtgatttt tgaagtccag cagacgccag
     6481 ccttcatcaa taacgttcag tttcttgagg ttacgcggcg tgcggtacat cctgttctcg
     6541 atgtagataa tcagggagaa catcaccgca accagcagtg acggacggtc ttccagtccg
     6601 cccagctcca gcaccaccat ttttgcgtca tcccgcaggg acggctcatc agagttaaaa
     6661 tactggccgt aagtgccgtt ggcagtgtac tggtcaagca gcacaatcat ttcgtccaga
     6721 cggctgcgga tagtcggtga ctcggcatac tgctcgctgt cactggcgtt tttcaggaaa
     6781 tccaccacgt catcaatacg tgctctgttc tctttggcca gccaggaggc tctgaccgcc
     6841 tgcaacagca gaccttcatg cacttcatcc aggttaccgt tggggctggc catcactgac
     6901 aactggtcac ggacacgctc cgctgactgg tcaatatcgg tgatgttcgc aaacgggtta
     6961 aaacgcaggg tttcaccgtc cagatacacc ccgcccatgt tctcacacag ggacttgtag
     7021 ccatccccca tgtcgaacac cacggcaaag cctccggagt ccagcacgct gcggataagt
     7081 ggctgtatca gcccggtttt accggccccg gaggtgccac agaccgccat gttgtagttg
     7141 gtgttattca tcccccggaa gaaaatatcg ataaacgcca gctggttacg gtaggtgggt
     7201 gccagcagac ctgccggtgt cagggggtta tccgccacta acggcatcag gttggcaaca
     7261 ttaaagctct ctgcgcgctg aaccactccg gcctctttca gctgtttaaa cagccctttc
     7321 ccggccataa agggcaggca ggtcaggaaa ttgcgcatgt ggttaaagcg cggtgaaatc
     7381 agctcaaaac cgtttttacg aaagctgttc aggatgtcct gctccacttc cagcgccgtt
     7441 tcattattgt ccttgcagaa tgctgtgatg ttgaggaagt aggacaccac ggaggactga
     7501 ccggagccca gccgctgacg cagttccccc cactccttcg cctctttctc cacggacgga
     7561 aaccatttgg cataggaggt cttcgacttt ttctccaggt ccatgtactt caggttggct
     7621 tcactgtggg ttttcacctg gtcttccacc accagagtca gcgtcaggat gaacggacag
     7681 gagatggaca gttccgggtt cagcaggttg ctgtagttgt cggccatgtt ccacaggaag
     7741 gcgatttccg ggttacgggc cagatggaaa ttcaggatgc gggccgtgct gttcctgccg
     7801 ttctcacgca ggcccagcgt caggtaatca gcccggactt tcagatcaaa actgtcctcc
     7861 acacactgat agttcagatc agaatacggg tccagctgac gtcttttcgg gtacagggag
     7921 tccgggttat ggttaatcat ctccccgaca atatcgataa aggcctgtgc atccaccgtc
     7981 tgtgtggtga tactggcccc ctgtaacgac gcccggatga ttttcaccag gttttccatt
     8041 tccagaatgt cggcccggct ttttttcttc gaaggagaac agtacgagat aaacacccgg
     8101 taatggcgca gggtcagggg cagattcatc ccctccggca gcggaaactg tgtcgctgcc
     8161 gctttcatat aataggcccg ggtaatggcg ttaaaccgtt cagcctgttc acctgaccag
     8221 gagaactcac gcagcccgta ttcaatcctg tcaccaacca gctgactgga catcagatgg
     8281 atacacaacg gaataccgcg cggcagtttg gtgcgcagca tgtgatcaag ggcctccaca
     8341 atggactcat tcgccccatt gatgggaatg gcttccagca taaagcccat cgtggtgtca
     8401 ttcatgaaca gaccggattc ctggttgtaa tcacggtacg gcagcagacg gctgaactgc
     8461 gggaagctca tttcgcccag aacttcattg gcctttgcgg attcgtcagg cagtttcagt
     8521 gctgtgacga gggagttaac cgcctgagtg acggcctcaa gtgggttatt cacatgaatt
     8581 ccttagttga aaaataaagc gagaaatgcg gtagccatga agggaatacc tgtaattaac
     8641 gcgccatttc ccggcgcata tacaaagatt tctgttttct gaatatccac tcatgttcat
     8701 ccggaattaa atcactatgt tctgcgactg acttatgcat aatgttttct ctgtctttcc
     8761 tgatacgcct gacattcaac gcacaacgtc acaccgggaa atattttccg ccaggcttcc
     8821 ggaataggat ttccgcatgc ttcacagaga taaacaggaa taccatgagc atttattttc
     8881 tgtcgtatcc ggtttattcc tgtcatggtc agttgttctg tcactgaata tgcttcatca
     8941 ctcacggaat attcctcccg acatgttctg acacgctacc gcccatccct tttcacgcac
     9001 agggcactcg ttttacaggc agattttgaa tacagaaagc ccgccggcat tgccggctga
     9061 tatacaactt cagggccagt taattaatac gtggttttcc ccacgcagac ggtttaataa
     9121 caaaaaacac gctggatggc tgatgataaa catcctggtt atcaatataa ggcgctatcc
     9181 ataatgctgc cgtctgctca cccgttctta gcggacgagg aggtgttacc ggcgtaactg
     9241 aactgaccgg aacaaccgtt ttcacttccc gggcagcggt aaacagcggg cgtggtgcca
     9301 gcagtttctg ctccggatgt gctgtcaccg caggtctgct gccggacgga gtggtcgcgg
     9361 tgacggtctg caccggcatt gtccggaagt tcccttcagc caggcgcggc agtgatgccg
     9421 caaccggctt tgcttctgag gactgctcca gttttttggc cttctcattg gcctgcacca
     9481 tcgtcataca ggtatcggat gtggtggcgt tacactcaaa ttccgtactg gttccggcac
     9541 agccggaaag taataaggtc cccagcagag gaataaataa agaaatctgt ttcattgtcc
     9601 ctcacctttc tcatcgcagt tcacaaaatc cttcttcaaa tccctgaccg tacaggtttt
     9661 tacaatgtca ggattttcaa aaaccgtact ccgtgatgat ttataaacat atgccacaat
     9721 gcgttttccc ccgcatcagg ttctggtgca ctgacttcac ataacggagg tgttattccg
     9781 gactcataca gcaccggata atttattttt ttgacgctgc catcagaaac cagcttattc
     9841 atcaaagggt tccttatatt tctgaattct tcatcacacc gacagcagtc atattaaaat
     9901 tgtccggcaa tgactcagaa ccgaatttct ccggtgcagg aagtacgatt cttgtggcgc
     9961 agttactgag aacagcatgc ataaaatcat ctgacacggt tttacccggc acagagaggg
    10021 ctgtaataac attaatatta cgcatgttca tttttatctc cgttgtttct gtttgttctg
    10081 tcatcaggaa gataaacagg gcatttcctg atttttcccg gcagggaagc gtcttccgta
    10141 taaacttcct tcagatgttc atctgcggtt accacccgga tacatttcat ttttccgggt
    10201 cgcaccggat atttcccggt gatcgctgcc agcgagacat acatcagtgc cagtgttgcc
    10261 cataatgcgg tgaccagttg catcagtgac cggtcagtct ccctgacata atctgcagca
    10321 tcagccgcgc ggtaatggca gatatcctgt atttctgatc tgcctgaaga atcagaggaa
    10381 gaaaaccagc attcactgac cgggaactgt ccggcaggtg ctgtttgtct tacgaagtta
    10441 atctccttaa ccagtaattt ccccggcgtg gtgttgtcct tccataaaac aaacagagcc
    10501 atcagggcca ttgtggccag tggcagaatt acggtgtact tcagacacca gaacagtcct
    10561 cgcagaaccc gggcgacaac ataccgggcg gcatggcacg cctgacggga cgacatatta
    10621 tttgccatcg ttgccccctc cctggctgcc ggacataaac tgctgcggat tcaggttgcc
    10681 cagctgcttc agcatctggt cagtattaaa gccgttaaga tgactttcag ccgatggcgg
    10741 aacaggaacc ggactttccg gattgtcttc ttccgctctg ctctgcgtgc gttccagcgc
    10801 catctcttcc acggttttca gctggaagcc gtcctggaac accacggtca cttcgttgcc
    10861 cgcaccaatc ggtatcaccg ggtgatactg ttcggcacgt ttgatgtagt agtcactgag
    10921 cgtctgtgcg gctttcgatg cgccgccacc gatacccatt ttcaggacat caccagcccc
    10981 gtaagcggct gtggcaccca gcccgacagc cggctgggag gcgcgctcca ttccctgacc
    11041 gataccgtca acaaatcccg cgccccatgc ccagccgagg attttgccgt tacgcatcac
    11101 cactccgccc ttgataccgt ttttaccccg gaagctgaca tgccccttaa tcggcatatc
    11161 aatagttttg ccgtccttca ggcaactgat attgcgggtg cgtacaatgg cacgctcact
    11221 ggacacatcc ccccaggctt ccagacccac aaaacatccc gttgcgtcat acgtcttgct
    11281 gttcggcatt tccaccaggc cggtgatacg cagctgcatc ggcaccgtgg attcattacc
    11341 ggtgacagag gcattggcat ccgccccttc aatcagcatg gctttcgcaa aacttcctga
    11401 cggaatgtac ggcagtgatg gtccctgttt tccctcatta cgggtaaaca ccttacgctg
    11461 tatccggtta ggcagcggca cagactggta cgtcacctgt ggtggtggcg tgacaccatt
    11521 cccgggataa aacgccgtcg gcggtggaac agcaacgctg ccctgcggcg ggaaggatac
    11581 gggggtgttt cctggctgtg gttcgccttc cgggccgggc ggtgaggcag gcatctgcgg
    11641 tacaggctca ccggtcgccg tgacgggatt agcacccagg gcttttacct gctctgccag
    11701 ggcggcattg tcctgtccca gcttttcaat acgacgctgg tcatcaccgc gctgtttgtt
    11761 cagaacatcc agctcacgac ggatttcctc atactgcttc tgcatctgcg ctgccgtcac
    11821 ctgcatctct gtggtggcat gctgacgcac cttgtcatca aaggtcgtat ccaccacacc
    11881 ggtcatatcc ggcacaggct cctgttcagc cacggtttca ccgttaccgg acatgtccac
    11941 atcagacaga tacagtgccc caccaatcgc ggaggctgtg ccgacaacca caatccccag
    12001 ccacaggtac tgcttgcgtt tcacaatggt attgatactg gccatcagtt gccctccccg
    12061 tcacggatga catacacatc catgcgcgca cctgccagta actggctggc aggctggctg
    12121 aacatcacgg cacgggttcc cggctgccag aagtcacttt cccggatatt cagggcagac
    12181 agcattttat tttccacggc aaagcggacc atcttcaggt gattccccgt ccatacggca
    12241 tctgccactg aagacagccc cgccggggcc tgcagggttt cctttgtcac cgggacctga
    12301 taccagcctg cgggtaattt tccgccacgg acggcctggc tgatggtcac aagcagggat
    12361 tcgtaaggcg tggacgtttc ccacgcaccg gcttcttctc cggttccgcg caggtcactg
    12421 accagctgaa tggtacgccc cgcgccttca cggggaacgg cctgaatgga aagattcaga
    12481 ccacgttctg tttccagaat gaacgtaaag ggctttttgt tgacggtggc aaccaccaca
    12541 ccgccggagg cggtctgctc attattggtc agggcaccat ccagactgtt cacggcgata
    12601 atccggtcac cgggaacggt aaacagattc gggctggtat tgctgatggc aatactggcc
    12661 tgaccaccgt tcaccattgg caccacggtg ggggccagcg taccgtttgc ggccatcacg
    12721 ctgcaggaaa aaaacaggct gccgaatatt attgccgtat tattttttct catcgtctgt
    12781 ttccccgaaa ttatccagcc aggtcacccc gttttcccgc ttcagaataa ggatgtaatg
    12841 tttgatatca gtgaaaggtt ttgaatcacc aatccaagtt ttaagcacac cacgaatttc
    12901 cacacggcca tactgaggcc agacacgaac actggtctgg aaaaaggctg agttcacgtt
    12961 atcgttttta atacgcttcg cttcttcagc cagaataact ttcatctggt tctgtgcgcc
    13021 cgggcggata tattgcagaa gcgcctgatg tgaggcatcg acggtttctg atgaaacatt
    13081 cagacggagg gcaataaatg acagcgtcat ctgctgtaaa taagaggcgt cggcactgtt
    13141 ctgtgacacg gcaaaggggg cattaaatgc cattggcgtc acggcagtgc gttgctcatt
    13201 ctgaagacgg tagttattca ccccctgaat gacgttaaca gagaggctga gaacaataag
    13261 cactgacata aatataaagg cgatggccat tacacgactg gtacttaaac gggcaccgtg
    13321 ttccatataa caatcctggt atcagttcta tttaatccac tgccggaaac acgaatcggg
    13381 aacattatga aaaataccgc gcagcagggc tgttggcata taccagtaaa tcaggtcacg
    13441 taaccaggaa ctgccccgcc cttttttcag ttttttaatc ccgaaataaa ccagaaccgc
    13501 tgcaccaata ccgaacagat atttcgatgt tgtgataccc cagccaatac agattgctgc
    13561 ggggatcagt tcatccagtg gcaggccaaa ccagcggctc tggttggtca gtgtttccgg
    13621 gaaacgatat ttcttaagtt tattctcgtc tcccgacatc gttttatttc ctgtcagagg
    13681 ccaacgacgg ccatacccac agcaataaat acagagatga tggcaaaacc ggctaggaac
    13741 ttgacgtttt tggtcatcat gtacatgaca gcaccgacca gaacttcagc cagaacaacc
    13801 catttaacaa cactggagtc cttaccgaag gtcgccttaa ccgtggtgtt accgcttgcc
    13861 atcaggtcct gaccactgct gccggcggcc atcgccagct gcgggaagaa catcatcaga
    13921 acagcagccg ggatcacagc gcgagccagg cgaagcatat tcagacgagt gaatttggaa
    13981 aaaaacgatt tctttttgac gggcgcagaa gcaccctgaa cacttaaaac ggcgtccata
    14041 tgattcctct gtttaatatt gatagataaa aactaaagtt ctttaaatgt tgactcgctc
    14101 tcttcggtca cctccctgaa tatttcttcg ttatagaaat caggaaaccg tttcagatga
    14161 tctctgagac ggatctggac ttctatcgtt ttacttcttc cactacgatc tttggcctta
    14221 ataagtcggt tatttgtatc ttc
//
LOCUS       DADSZD010000112        14230 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.113, whole genome shotgun sequence.
ACCESSION   DADSZD010000112 DADSZD010000000
VERSION     DADSZD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.113"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(71..1720)
                     /gene="pgi"
                     /locus_tag="J1Z28_003099"
     CDS             complement(71..1720)
                     /gene="pgi"
                     /locus_tag="J1Z28_003099"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBA7717911.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2245..3594
                     /gene="lysC"
                     /locus_tag="J1Z28_003100"
     CDS             2245..3594
                     /gene="lysC"
                     /locus_tag="J1Z28_003100"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBA7717912.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3847..4119
                     /locus_tag="J1Z28_003101"
     CDS             3847..4119
                     /locus_tag="J1Z28_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBA7717913.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4120..4992)
                     /gene="rluF"
                     /locus_tag="J1Z28_003102"
     CDS             complement(4120..4992)
                     /gene="rluF"
                     /locus_tag="J1Z28_003102"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBA7717914.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5340..6287
                     /locus_tag="J1Z28_003103"
     CDS             5340..6287
                     /locus_tag="J1Z28_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBA7717915.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFSLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            6357..7163
                     /locus_tag="J1Z28_003104"
     CDS             6357..7163
                     /locus_tag="J1Z28_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7717916.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHGG
                     DGQYESHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7173..7580
                     /locus_tag="J1Z28_003105"
     CDS             7173..7580
                     /locus_tag="J1Z28_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose IIA component"
                     /protein_id="HBA7717917.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            7580..8074
                     /locus_tag="J1Z28_003106"
     CDS             7580..8074
                     /locus_tag="J1Z28_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBA7717918.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            8140..8937
                     /locus_tag="J1Z28_003107"
     CDS             8140..8937
                     /locus_tag="J1Z28_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA7717919.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            8948..9772
                     /locus_tag="J1Z28_003108"
     CDS             8948..9772
                     /locus_tag="J1Z28_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBA7717920.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9824..11053
                     /locus_tag="J1Z28_003109"
     CDS             9824..11053
                     /locus_tag="J1Z28_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBA7717921.1"
                     /translation="MKTTALRLYGKRDLRLEIFDLPEMQEDEILATVITDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAVILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            11435..12124
                     /gene="pepE"
                     /locus_tag="J1Z28_003110"
     CDS             11435..12124
                     /gene="pepE"
                     /locus_tag="J1Z28_003110"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBA7717922.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12214..13845)
                     /locus_tag="J1Z28_003111"
     CDS             complement(12214..13845)
                     /locus_tag="J1Z28_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBA7717923.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT         3478 a         3576 c         3703 g         3473 t
ORIGIN      
        1 tcacagacaa ggggatttat ctgataaaaa atgccgacat aacgtcggca tttttattaa
       61 ggttatttaa ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
      121 cgtggctact gatttctttg tcatctttca gctctggcag aatacggttc gccagctgtt
      181 tacccagctc cacgccccac tggtcgaagg tgaagatgtt cagaatcacg ccctgagtaa
      241 agattttgtg ctcatacagc gcaatcaacg cacccaggct gaacggagtg atttcgcgca
      301 gcaggataga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
      361 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
      421 caaacgccag cgcttcggtc tgagcgaaga agttagacag cagtttctgg tgatgatcag
      481 agagtgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
      541 ggtggatcag ctggtagaac gcgtgctgac cgttagtacc tggttcaccc cagataatcg
      601 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
      661 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
      721 cttcagtttc cgcaccaaag aaattgttgt accagatgcc gatcagcgcc agcaagacag
      781 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
      841 gcagttcaac aaaattatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
      901 acaaagagta acggccgcca acccagtccc agaactcgaa catgttggca gtatcaatac
      961 caaactcgcc aacggctttg gcattggttg agagcgccgc aaagtgtttc gcaacgtgtt
     1021 tttcatcacc agccgctttc aggaaccagt cacgcgcact atgggcgttg gtcatggttt
     1081 cctgagtggt gaaggtttta gatgctacca ggaacagcgt ggtttccggg tttacttttt
     1141 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     1201 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccgccgatcc
     1261 cgatgttcac tacgtcagtg attgctttgc cggtatatcc tttccactca ccggaaataa
     1321 tcgcttccga gaaggttttc atcttctcca gcactgcgtt gacttccggc attacgtctt
     1381 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     1441 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     1501 cgcactcttt cgccagatct tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     1561 agtaatccac cagcatctga tcgtcgaagg ttgcggagaa cttagaaaaa cgatcgccat
     1621 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     1681 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     1741 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     1801 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     1861 actgaccgcc aggagtggat gaaaaatccg catgacccca tcgttgacaa ccgccccgct
     1921 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     1981 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     2041 tgattgaacg gctagccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     2101 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     2161 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     2221 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     2281 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     2341 gtgcgtttag ttgtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagct
     2401 gaaggactgg aacctggcga gcgattcgaa aaactcgacg caatccgcaa tatccagttt
     2461 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     2521 gagaatatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc cctgacagat
     2581 gaactggtca gccacggcga gctgatgtcg accctgctgt ttgtcgagat cctgcgcgaa
     2641 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgatcgattt
     2701 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgcttcca
     2761 cgcctcaatg aaggcttagt gatcacccag ggatttatcg gcagcgaaaa taaaggtcgt
     2821 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     2881 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     2941 gtggtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     3001 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     3061 ccagtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     3121 actgaaaatc cgccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     3181 ttgcacagcc tgaatatgct gcattctcgc ggtttcctcg cggaagtttt cggcatcctc
     3241 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagtgt ggcattaacc
     3301 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc cctgctgatg
     3361 gagctttccg cactgtgccg ggtggaagtg gaagagggtc tggcgctggt cgcgttgatt
     3421 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaacca
     3481 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     3541 ggcgaagatg ccgagcaggt agtgcagaag ctgcatttta atttgtttga gtaaacacag
     3601 tatggcccgg aagacattat ttcgggccgt attgtttttc ttgtcattat gcccataaat
     3661 aaacgagtct attccctgtt aaccagcgtc tttatcggag aataattgcc tttaattttt
     3721 tatccgcatc tctaactaat tatcgaaaga gataaatagt taagagaagg caaaatgaat
     3781 attatcagtt ctgctcgcaa acgaattccc gtacactgaa ctctctggct cactgagggc
     3841 accatcatgg cactcccccg tattacccaa aaagagatga ctgaacgcga gcagcgtgaa
     3901 cttaaaacac tgctggatcg ggcgcggatt gcgcatggtc gcgtactgac caactccgaa
     3961 actaacagca ttaagaaaga gtacatcgat aaattaatgg tcgagcgtga ggctgaagcg
     4021 aaaaaagccc gccaattgaa gaaaaagcag gcttataaac cggatcctga ggcgtcattt
     4081 agctggtcag caaatacgtc gacgcgcggc agacgctgat taacgcccct tctttttgcg
     4141 ccccggcgag gtaaaacgct taccgttgga cgccgggcga ccgccttttt ccgccgtttt
     4201 ttccatctta acgactggac gtttgatgcc taccgttttc ggtttggcct tcgctttagg
     4261 ttttgcctcg gaagaggaat tttcaatgag cttaaagaga tcgattaact catcgtcggt
     4321 taaatcgcgc cactcaccca gcggaatgcc gcttaagctg acgttcataa tgcgcgtacg
     4381 ttccagcttt ttcacttcgt aaccaaagtg ctcgcacatg cgacgaatct gacggttcag
     4441 cccctgcacc aaggtaatgc ggaagacaaa cggtgcttct tttttgactt tgcacttttt
     4501 agttaccgtc ccgaggatcg gtactcccgc ccccatacca cgaataaatt catcggtaat
     4561 cggtttatca accgtcacca gatactcttt ctcatgatcg ttacccgcac ggaggatctt
     4621 attcaccaga tcgccgtgat tggtgaggaa aatcagcccc tgggaatctt tatccaggcg
     4681 gccaatcggg aacacgcgtt tgctgtggtt aacgaaatcg acaatgttat cgcgctcgcc
     4741 atcttcggtg gtgctgacaa taccgacggg cttgttcagg gcgataagta ccaaatcttc
     4801 ggattcccga ggctcaatca actgaccatt tactttcaca acgtcgccgg gtttcacctg
     4861 atcgccaatg gtggctcgct tgccattaag gaacacattg ccttgctcga tatagcgatc
     4921 cgcttcgcgg cgtgagcaaa ttccgctttc gctgatgtat ttatttaaac ggactgatga
     4981 gtcgggcagc ataaattctc ctgtaaaacg cggaatatac cgtacctctt agcggggaaa
     5041 aacagggtta tgcggcaacg tcgaaattaa atgtgatctg ccacgccttt tatcacaaaa
     5101 gtgtctccgg aagtacgatg caaatttgcc tggcaaaata agaataatca acaaattacg
     5161 catcgctgca cacctgtgct gatgactact acgcagagaa acaaatgtgc aagagaaagc
     5221 gctattttcc cccatgcaaa gagtgaaaaa tcgctttgaa aggccatcat gatggggtta
     5281 tatcagatca agcggtgaga aagcgtattt gcacaaatgt gcagaaccag gaggcggcaa
     5341 tggaaaacag tgacgatatc cgtttgattg tgaagattgc ccaactctat tacgaacagg
     5401 atatgacgca ggcgcaaatt gcgcgcgaac tgggtattta ccgcaccacc atcagccgct
     5461 tgcttaaacg aggccgcgat cagggaattg tcaccatcgc catcaactat gactacaacg
     5521 aaaatctctg gctggagcag caagtgaagc aaaagtttag cctgaaagac gttgtggtgg
     5581 tgtcaggaaa tgatgaggat gaagatactc aactggcgat gatggggtta cacggcgcgc
     5641 aactgctgga tcgcttgctg gaacctggcg atattgtcgg tttttcctgg ggccgcgcgg
     5701 tgagcgcact ggttgaaaac ttgccgcagg cggggcaatc gcggcagtta atttgcgtgc
     5761 cgattattgg cggcccgtcc ggtaaactcg aaagccgcta tcacgtaaac acattaacct
     5821 acagcgcggc agcgaagctg aaaggggaat cgcatctcgc ggattttccg gctctgctgg
     5881 ataacccatt aattcgtaat gggatcatgc agtctcagca ctttaaaacc atctctgcct
     5941 actgggataa tctagatgtc gccctggtgg gaattggctc accggccatt cgcgacggcg
     6001 ctaactggca tgcgttttat ggtggtgaag agagtgacga cctgaatgcc cgccaggttg
     6061 ctggcgatat ttgctcgcgc ttttttgata ttcacggcgc aatggttgaa acgaatatga
     6121 gcgaaaaaac actctctatc gaaatgaata aattaaagca ggcacggtat tccattggca
     6181 ttgccatgag cgaagaaaaa tacagcggaa ttgttggtgc actgcgtgga aaatatatta
     6241 attgtctggt aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat
     6301 gtttaccgta ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc
     6361 aaacgtggtt aaatttgcag gataaaatca ttattgtcac cggtggcgca tccggcattg
     6421 gtctggcgat tgtggatgaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc
     6481 acggtggcga tggtcaatat gaaagccata aaggttatca gttctggccg accgatattt
     6541 ccagcgccaa agaggtaaat catacggtag cagaaattat ccagcgtttt ggtcgcatcg
     6601 acggtctggt taacaacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc
     6661 ctgccgggca gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga
     6721 aaggcgtttt tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg
     6781 tgattgtgaa tgtttcctca gaaagtgggc tggaaggctc agaaggccaa agctgttacg
     6841 ccgcgaccaa agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaagc
     6901 acggtatccg tgtggtcggt atcgcgccgg ggattctgga aaaaacagga ctgcgcacgc
     6961 cggaatatga agaagcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag
     7021 gctataccaa aaacgccatt cctattgggc gcgccggaag attagcagaa gtggctgatt
     7081 ttgtttgtta tctgctgtct gaacgcgcca gctatatcac cggagtaacc actaacattg
     7141 cgggcggcaa aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcgcatg
     7201 gcaaattagc ttgcgccatg ctggaatcgg ttcagatggt gtacggcgat gccagggtcg
     7261 aggccgttga atttgtgccc ggcgagaacg ccggagacat cgtagcaaag ctggaaaagt
     7321 tagtaagcat tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc
     7381 cgtggaatgc ggcagcgatg cttgcgatgc gcaatcctcg tctgcgggta atcagcggcc
     7441 tttctctgcc gctggcgctc gaactggttg ataaccagga cagcatgaat attgatgaac
     7501 tgtgtgaaca cctgacgcaa atcgcaaagc aaacctgcgt cgtctggaga caggtggcaa
     7561 cagcggagga ggatttctga tgaacattac gctcgcccgc attgatgacc gcctgatcca
     7621 cggccaggtc actaccgtct ggtctaaagt ggctaacgcc cagcgcatta ttatctgcaa
     7681 tgacgaagtt tataacgatg aagttcgccg gacattattg cgtcaggctg cgccaccggg
     7741 aatgaaggtt aacgtggtca atattgaaaa agccgtcgcg gtgtatcaca acccgcaata
     7801 tcaggacgaa accgtttttt atcttttcac tcgcccacag gatgccttag caatggttcg
     7861 ccagggggta aaaataggta ccctgaatat tggcggcatg gcctggcgtc ctggtaaaaa
     7921 acagttaacc aaagcagttt cattagatga cgatgatatt aacgcatttc atgagttaaa
     7981 taatcttggc gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat
     8041 cgacaaaata aacgaacagt taatcgcgaa ttaaataata gcgcctgtgt acatatgctt
     8101 taacaggcag ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa
     8161 atcattgcta tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt
     8221 cagactcatc gtccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa
     8281 accggaatta tgctcggtgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc
     8341 gcggcgcaat ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt
     8401 ggtcagcaaa gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag
     8461 gtgctgacgg tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca
     8521 gcggaagaag cacggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag
     8581 gcactgcgcg ttgctattcc ggcactgatt gtctcgctgt tcgtcagcgc tgatatggtg
     8641 agcaatatgc tgagcgccat tcccgaattt gtgacccgtg gactgcagat tgctggcggt
     8701 tttatcgtgg tggtcggtta cgccatggtg cttcgcatga tgggcgtgaa atatttgatg
     8761 cctttctttt tcctcggttt cctcgcaggt ggctacctcg atctcagtct gctggcgttc
     8821 ggtggcgtcg gcgtgatcat ggccctgctc tacatccagt taaatccaca gtggcgtaaa
     8881 gctgaaccac atccccagac caccactatc accgcccttg accaacttga tgattaacgg
     8941 agccatcatg gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc
     9001 caacctgcaa caggcatcct ttaactttga acgtattcac gggctgggtt tttgctacga
     9061 catgatcccc gccatcaaac gactttaccc attaaaagag gatcaggttg cggcgctcag
     9121 gcgacaccta gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgtcac
     9181 tgccgccatg gaagaggcgc gagccaacgg cgcggaaatt gatgacggta ccattaacgg
     9241 catcaaagtc ggtctgatgg gaccattggc aggagttggc gatccactgg tctggggaac
     9301 gctgcgcccg attaccgccg cgctcggcgc atctctggca ctttcgggca acattctcgg
     9361 ccccctgctg ttcttcttta ttttcaacgc ggtgcgtctg gcgatgaagt ggtatggcct
     9421 acagctcggc tttcgcaaag gggtgaatat cgtcagcgat atgggcggta atttgctgca
     9481 aaaactcacc gaaggcgcgt cgattctcgg gctgtttgtg atgggcgtgc tggtgaccaa
     9541 atggacgtca atcaacgtac cgttggtggt ttcacaaacg cctgccgccg atggtgccac
     9601 cgtcaccata accgtgcaga acatcctcga ccaactttgc cctggtttgc tggcgctcgg
     9661 tctgacgctg ctgatggttc gtctgctcaa caaaaaaatt aacccggtat ggctgatttt
     9721 cgccctgttt ggtttaggga ttatcggtaa tgcgctgggc ttcctgtcct gattcttcgc
     9781 cccggcgcaa ccgtcggggc catcgttcaa cacgaggtgg tttatgaaaa caacagctct
     9841 gcgtctttat ggtaaacgtg atttacgcct ggaaatcttt gaccttcctg aaatgcagga
     9901 ggatgaaatc ctcgcgacgg tgatcactga cagcctgtgc ctctcttcct ggaaagaggc
     9961 caatctgggt gaaaaccata aaaaagtacc cgacgatgtg gcgaccaatc ccatcatcat
    10021 cggccacgaa ttttgcggcg atattctggc cgtgggtaaa aagtggcagc acaaattcca
    10081 gccgggtcag cgttatgtga ttcaggccaa cctgcaactc cccgaccgcc cggattgccc
    10141 cggctactcc ttcccgtggg taggcggcga agcgacgcat gtggttattc ccaacgaggt
    10201 catggaacaa gattgcctgc tggcatacga cggcgaaacc tattttgaag gctcgctggt
    10261 tgaaccgctt tcctgcgtga ttggcgcgtt caacgccaac tatcatcttc aggaaggtag
    10321 ttataaccac acgatgggga ttcgcccgca agggcgcacg ctgatcctcg gcggcaccgg
    10381 accaatggga ctgttggcga ttgattatgc gctacatgga cccgttaacc cttcgctgct
    10441 cgtcattacc gataccgaca acgacaaatt gagttatgca cgcaagcact atccatcaga
    10501 accgcaaaca ctgattcatt atctcaatgc cgccgatgca gcatttgata cgttaatggc
    10561 actgagtggc ggtcacggct tcgatgatat tttcgtcttt gtgcctaatg aaggactggt
    10621 gactctcgcc tcttccttgc tggcgacaga tggttgtctg aatttcttcg ccggaccgca
    10681 ggataagcac ttcagcgcgc caattaattt ctacgatgtg cattatgcat ttacccacta
    10741 cgtgggcacg tcaggcggca ataccgacga catgcgcgca gcggtcaaat tgattgaaga
    10801 gaaaaaagtg caggccgcaa aagtggtaac acatattctt gggctgaatg ccgcgggcga
    10861 aactacgctt gaattgcctg ccgtcggcgg cggcaaaaag ctggtgtata ccgggaaata
    10921 cctgccgctg acgtcactca cgcagattca ggatcaagca ctggcggtga ttctggcgcg
    10981 tcatcagggg atctggtccg gtgaggcgga gcaatacctg ctcgcccatg cagaggcaat
    11041 ttcccatgat taatcgcgct acttaactct gcatgtcgct ggctggtcgc cgcggcaact
    11101 tcgcgacctg atgtgcggct gattgctgaa gcggcacaat gtgcacgaga aggataaaaa
    11161 aacatcagcg ccttaagggg cgctcaaatt ggtggcaaag tgcacattgt ttatgccgga
    11221 tgcggaatga agccttattc ggcctacata atcttgaaaa ttcaatatat tgcagttatc
    11281 tggtaggcca ggtaagcgta gcgcatcagg caatcctgtg tttgtcatca gtctcagcgc
    11341 ctgtgcaata tgtgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca
    11401 ttccctccgt gttttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc
    11461 gacgctgccg ggtaaagcct ggctggaaca cgcgctgccg ctgattgctg aacagttgca
    11521 gggtcgccgt tctgcggtat ttattccttt cgctggcgta acgcagacct gggatgatta
    11581 cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag
    11641 cgttgtcgat cccgttgccg cgattgaaaa tgcggaaatt gtgattgtcg gcggcggtaa
    11701 tactttccag ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt
    11761 ggtgaaacgt ggcgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac
    11821 cattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct
    11881 gttcccgcta caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga
    11941 aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg
    12001 tctgccggaa ggtaactgga tcacagtaag taaaggtcac gctacgctgg gtggcccgaa
    12061 caccacttat gtgtttaagg ctggtgaaga agcggtgccg ctggaagcgg gtcaccgttt
    12121 ttaagtgaat tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgatgctt
    12181 tacgcgtctt atcaggcctt cagggtcacg gtattaatac tcgtcccgtc cttcatcttc
    12241 atccggctgt tccagcacac tgtaagccac cgaacaaaac agcgagttca gacgctgcat
    12301 atctcccagt aagcctaaat gtagcgaact ggtttcaata ctctgcacgt tttgctggtg
    12361 caggcgatcg acgtgggcgt gggaatagcg gcgattaaga atgcgaaaac gatgtttgct
    12421 gcggcgcaaa cgacgggcgc tggtaacatc gccagagaag aaaaccgaca ttgccagttt
    12481 taaattactg agcaattgct catagagcgc atccagttcc ttcaagccat caagcgaaaa
    12541 tgctcgcctt gctgccagcg atttgtcggc aatttcactc cccatgcgct cgacgatatc
    12601 ggaggcctgt tcaaggttga gcgacatttc gatgatctcc gcccagcggc gcgactcttc
    12661 ttctgccagt tcctcttttg gcatccgcgc cagatacagc ttaatggcgg tatagagaac
    12721 gttgatgtca tcagccagct tacgcagctc tttctcctgc cgtggctcgc cgtgcatcac
    12781 tttattcaac ccttccatca tctgttccat ggcgtcgcca atgcgcaggg tttcgcgggc
    12841 agcgttcgcc agagcaagcg tgggcgtatc cagcgcgctg acatccagat gtttaggccg
    12901 tagctgggta tccagttccg gttcatcgcg aataatcgtt ttgcaaaacc gtgccatcgg
    12961 gtcaacgaat ggcaacatga ccaggcagcg tacaaggttg tagaagacgt ggaaatagat
    13021 caccaattcc gcttttggca atggcaactt ccccattgtc tctgccagca aatgaacaaa
    13081 cggcaggata atcaggctac ccaccagctt aaacagcagg ctacccagcg cgacacggcg
    13141 ggctgcggca ttggcggcac tgttgttgag catcgccagc aggccggaac cgaggtttgc
    13201 accaatcacc agacaaagcg ccactgggaa agagataatg cctgcggcgg tcagagtcgc
    13261 ggtcagcagt accgcggcaa ggctggagta gctgataatg gcgaacatcg caccaatcag
    13321 cgcatccagc aaaatgtcgc ctgtcagcga ggcaaagatc acctgaacgc cgtttgcctg
    13381 agtgatcggc gttacggcct gcacaatcaa ctccagcgcc agtaaaatca gcccaagacc
    13441 aataccgacg cggcccagct gcccggcgcg tgactgtttg cgtccgagga aaaaaatcac
    13501 gccgataaaa ataagtaacg gtgacagcca ggataaatcg aaggtgagaa tacgcgccat
    13561 cagcgccgtc ccgacatctg cacccagcac aatcaccagc gcaggcgcga gagcaaccag
    13621 atcctgagcg acgaacgagg tcaccagcat ggtggtggca ttactgctct gcaccagtgc
    13681 ggtaacgccg atccccgcgc aaaaggcgag cggctttttt tcgacgctcc ggctaaggac
    13741 agtacgcaga cgcgcgccga agacgcgcat tacgcctgtt cgaacaatat gagtccccca
    13801 gaccagcagg gcgacggcag aaagcaggtg aagcaaagtt agcacggaat caggttctcc
    13861 tttttgttat gcattttcct gttaagattt ttctaatcac gttgttttta tccaggcatt
    13921 tctcttgtat gtactttcag tataagagat ttatacaaga aagcatttaa aaaggcattt
    13981 atataaaaat ccacctgtat ctgttgctat ttctgtcaca ctttcccctg tcgtttacac
    14041 cagataatcg ttgtcacttt tgtgacctct gagaagataa acaacgaaat atttaagacc
    14101 agttcctgtg aatgaaaata attaagtaac aacggtatta cggagattga gcagcttacg
    14161 gtagcaaaac gcataaactc ctgaatcgcc aaaaaagaga aactaaataa tttttgtctt
    14221 aaaaaacatt
//
LOCUS       DADSZD010000113        13878 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.114, whole genome shotgun sequence.
ACCESSION   DADSZD010000113 DADSZD010000000
VERSION     DADSZD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.114"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            2..517
                     /gene="hpt"
                     /locus_tag="J1Z28_003112"
     CDS             2..517
                     /gene="hpt"
                     /locus_tag="J1Z28_003112"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBA7717924.1"
                     /translation="MIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRGSFMFMAD
                     LCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDIIDSGNTLS
                     KVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDYAQRYRHL
                     PYIGKVILLDE"
     gene            complement(558..1220)
                     /gene="can"
                     /locus_tag="J1Z28_003113"
     CDS             complement(558..1220)
                     /gene="can"
                     /locus_tag="J1Z28_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBA7717925.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            1329..2255
                     /locus_tag="J1Z28_003114"
     CDS             1329..2255
                     /locus_tag="J1Z28_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7717926.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            2252..3022
                     /locus_tag="J1Z28_003115"
     CDS             2252..3022
                     /locus_tag="J1Z28_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7717927.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            3127..3567
                     /locus_tag="J1Z28_003116"
     CDS             3127..3567
                     /locus_tag="J1Z28_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7717928.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLTSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFREHIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            3631..4860
                     /locus_tag="J1Z28_003117"
     CDS             3631..4860
                     /locus_tag="J1Z28_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBA7717929.1"
                     /translation="MYKQAVILLLMLFTASVSAALPAHYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(4864..5244)
                     /gene="panD"
                     /locus_tag="J1Z28_003118"
     CDS             complement(4864..5244)
                     /gene="panD"
                     /locus_tag="J1Z28_003118"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBA7717930.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            5518..6402
                     /locus_tag="J1Z28_003119"
     CDS             5518..6402
                     /locus_tag="J1Z28_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBA7717931.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGESMMTLAQWFEEKGIQQGRQEVSQEFALRLLSKGMSRE
                     DVAEMVNLPLAEIDKMIN"
     gene            complement(6484..7335)
                     /gene="panC"
                     /locus_tag="J1Z28_003120"
     CDS             complement(6484..7335)
                     /gene="panC"
                     /locus_tag="J1Z28_003120"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBA7717932.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMNLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRSDDIQIRDADTLLEISENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(7347..8141)
                     /gene="panB"
                     /locus_tag="J1Z28_003121"
     CDS             complement(7347..8141)
                     /gene="panB"
                     /locus_tag="J1Z28_003121"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBA7717933.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFAEEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            complement(8253..9515)
                     /locus_tag="J1Z28_003122"
     CDS             complement(8253..9515)
                     /locus_tag="J1Z28_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBA7717934.1"
                     /translation="MKTFFRYFLFLALCSCCYTASAGTDDDVGYIVGNNYGVGPSDQK
                     WRETGPNGDVTVKFRYGSGTNNLVFYKPTQLGPTGVSLKWAQLDSASGGGFLYCNRSS
                     NSSGAPMSIEHKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSTLTSTDINPSGMYI
                     GDSTSQSFNWRGESEQTLYWSCNNANSSKKYWAVGGVMQTLTIEFYTDTDFNPTTNQR
                     VTLSRTDSYLYSFKAYNAGVSIKSYFLKIDFDLTDIVLTNPTCFTAALSGPSVSGSTV
                     KMGDYSPAQIKNGATAVPFDITLQNCIRVRNIETKLKSNKVGSVSKELLANTLTGNDA
                     AKGVGVLIEGLKNTKSAQMVLKPNDATSIYKDYETENDTTGGIFPDNGNGGTSQPLHF
                     QATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            complement(9565..10161)
                     /locus_tag="J1Z28_003123"
     CDS             complement(9565..10161)
                     /locus_tag="J1Z28_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7717935.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHINF
                     RALSGVMATGSSQIFANEDNAGAKNVGVVIFSIQDPANTFNVLSATGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGPLISHVLVDIYYE"
     gene            complement(10188..10796)
                     /gene="yadL"
                     /locus_tag="J1Z28_003124"
     CDS             complement(10188..10796)
                     /gene="yadL"
                     /locus_tag="J1Z28_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBA7717936.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGTTKISTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTTR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTNVTAGDFTAPATFTVTYN"
     gene            complement(10808..11374)
                     /locus_tag="J1Z28_003125"
     CDS             complement(10808..11374)
                     /locus_tag="J1Z28_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7717937.1"
                     /translation="MIKITPHKITILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     GTNVTDNGNDKYTLVIPSMGMDKIANKTAQSEANFKLVANGCSSGISWIDTTLTGNQS
                     GSSPALIIPLASDTTSTTSYIGMGFKRKATSGDTFLKPNSAEYIRWSASEISTDGLEM
                     TVALRETSVGKGVPGKFRALATFNFSYQ"
     gene            complement(11391..13868)
                     /locus_tag="J1Z28_003126"
     CDS             complement(11391..13868)
                     /locus_tag="J1Z28_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HBA7717938.1"
                     /translation="MGKDASNIDLSRYTEGNPTLPGIYDVSVYVNDQPIMSQSIAFAV
                     IEGKKNAQACITQKNLLQFHISSPDKNSEKAILLKRDEDLGDCLNLAEMIPQSSIRYD
                     VNDQRLDIDVPQAWIMKNYQNYVDPSLWENGINAAMLSYNLNGYHSESPGRTNDSIYA
                     AFNGGINLGAWRLRASGNYNWMTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGET
                     FDSVSIRGIRLYSDSRMLPPVLASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAF
                     AIDDLSPSGYGSDLIVTIEEADGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSI
                     QDEPNLFQASYYYGLNNYLTGYTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVS
                     IPDDKTYQGQSYRISWNKLFENTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQE
                     LEPKSMRNYSRMKNQVTVSINQPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYS
                     NSASWGSYSISAQRSLNEDGQTDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDF
                     KGNNQLNISSSGYSDTNRISYSVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASA
                     SSDNSRQFSLNTDGGFVLHSGGLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDR
                     WGYGVTSALSPYHENRIALDINDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAI
                     MNIVRSDGKNIPFAADIYDEQNNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSC
                     FAHYQQNTTSEKIAQSIILNGLRCQIQ"
BASE COUNT         3450 a         3185 c         3395 g         3848 t
ORIGIN      
        1 aatgatcccc gaagcggaga ttaaagcgcg tatcgccgaa ctgggtcgtc agattactga
       61 gcgttacaaa gacagcggca gcgatatggt gctggtgggt ctgctgcgtg gctcatttat
      121 gtttatggcg gacctgtgcc gtgaagttca ggtatctcat gaagtcgact ttatgaccgc
      181 ctccagctac ggtagcggca tgtccaccac ccgtgatgtg aaaatcctca aagacctgga
      241 tgaagatatc cgtggcaagg acgtgctgat tgttgaagat atcatcgact cggggaatac
      301 actgtcgaaa gtgcgtgaga tcttaagcct gcgcgaaccg aagtcgttgg cgatttgtac
      361 gctgctggat aaaccgtccc gtcgtgaagt gaacgtcccg gtagaattta tcggtttctc
      421 gatcccggat gagtttgtgg tgggttacgg cattgattac gcacagcgtt accgtcatct
      481 gccgtatatc ggcaaagtga ttctgctgga cgagtaagtg tgaagttgcc ggatgtgttg
      541 catccggcat ggcattttta tttgtggttg gcgtgtttca gcttgaggtt ggagatcccg
      601 tgacggtaac gttgctcaag ggtttcgcgg ttagtcgctg tgacatccag atcacgcagc
      661 aagccgtcgt gaatgccgta ggcccagccg tgaatggtaa ctttctgccc gcgtttccac
      721 gctgattgca taatggtgga gtggcccagg ttatacacct gttccatgac gttcagttca
      781 cacaaggtat ccagacggcg ctcttgcggc atttcgccga gcaatgagct atgtttgaac
      841 cagatatcgc ggatatgcag cagccagttg ttgataagcc ccagttccgg gttttcaact
      901 gcggcttgta cgccgccgca accgtagtgg ccacagataa taatgtgttc aacttcgagt
      961 acatccactg catactgaac cacggaaagg cagttcaggt cggtgtgaat gaccaggtta
     1021 gcaacattac ggtgaacaaa gagttcgccc ggctcaagac cggttaaacg ttctgcagga
     1081 acgcgactgt cggaacatcc aatccataga aagcgcggtt tttgcgcttg tgccagtttc
     1141 tcaaaaaacc cgggatcctc ttccaccagc atttttgacc atagtgcatt gttgctgatg
     1201 agtgtatcta tgtctttcat ggaggttaac gacctgtaac caaataatta cgtttggcta
     1261 atatagggca actccgggac gatttaaacc acagataaag tgtaagaacg taaggtaagt
     1321 aaaaatttat gaccattgca ctggaacttc aacagcttaa aaaaacctat ccaggcggcg
     1381 ttcaggcgct tcgtggaatc gatttgcagg tcgaagcggg tgatttttat gcgcttctcg
     1441 ggccgaacgg ggccggaaaa tcgaccacta tcggtattat cagctctctg gtaaataaaa
     1501 cctccgggcg ggtcagcgta tttggttacg atctcgagaa agatgtcgta aacgctaaac
     1561 gtcagttggg tctggtgcca caggaattta acttcaaccc gtttgaaacc gtgcagcaaa
     1621 ttgtggtgaa tcaggcaggg tactacggtg tggagcgcaa agaagcgtac attcgcagcg
     1681 aaaagtatct taaacaactc gatctatggg gaaaacgcaa cgaacgtgcg cgtatgttat
     1741 ctggcgggat gaagcgccgt ttaatgattg cccgtgcgtt aatgcatgaa cctaaactac
     1801 tgattctcga cgaaccgacc gcaggcgtgg atattgaact tcgccgctca atgtggggct
     1861 ttttgaagga tttaaacgac aaaggcacca ccatcattct caccacacac tacctggaag
     1921 aagcagaaat gctgtgccgc aatatcggca ttattcaaca cggtgagttg gtggaaaata
     1981 cttcgatgaa ggcgctgctg gcgaagctga aatcagaaac ctttattctc gatctcgcac
     2041 cgaaaagtcc gttaccgaag ctcgatggct atcagtatcg actggtagat accgccacgc
     2101 tggaagttga agtgctgcgt gagcagggca tcaacagcgt atttacgcag ttaagtgagc
     2161 agggcattca ggtattaagt atgcgtaaca aagctaaccg tctggaagag ctgtttgttt
     2221 cactggttaa tgaaaaacaa ggagatcgca catgatgcat ctttactggg tggcgctaaa
     2281 aagcatctgg gcgaaagaga tccatcgctt tatgcgtatc tgggtgcaga cgctggtgcc
     2341 gccagtcatc accatgaccc tttactttat tatcttcggt aacctgattg gttcgcgtat
     2401 tggcgatatg catggcttca gctacatgca gttcatcgta ccggggctga tcatgatgtc
     2461 ggtgatcacc aacgcctacg ctaacgtcgc gtcatcattt tttggtgcca agttccagcg
     2521 taatattgaa gagctgctgg tagcgccggt tccgactcat gtcattattg ccggatatgt
     2581 cggtggtggc gtggcgcgtg gtctgtttgt tggtattttg gtgacggcaa tttcactgtt
     2641 ttttgtgccg tttcaggtgc attcgtgggt attcgttgcc ttaaccctgg tgctcactgc
     2701 ggtgctattc tcccttgcgg gtttgctgaa cggcgtgttt gccaaaacgt tcgatgacat
     2761 cagcctggtg ccaacctttg tgttaacgcc actcacgtat ttgggcgggg tgttttactc
     2821 cctgactttg ttgccgccgt tctggcaagg gctgtcgcac ctgaacccaa tcgtttatat
     2881 gatcagtggt ttccgctacg gcttcctcgg tatcaatgat gttccgctgg tcactacctt
     2941 tggcgtactg gtggtcttta ttgtggcgtt ttatttgatc tgttggtcgc tgatccaacg
     3001 tggacgtggt ttgcgtagct aaggctattt cctctcctct ggatttgggg gagaggagtt
     3061 ttgacggcta tcacccttta tcaacaatgg tcagggtaga ctgattttcg gctaaggagg
     3121 aaggcgatgt taggttgggt aattacctgt cacgatgacc gggcgcaaga gatactggat
     3181 gcgctggaga aaaaacatgg ggcacttctt cagtgccggg ccgtgaattt ctggcgcgga
     3241 ttaacctcta atatgctcag ccgcatgatg tgcgatgcct tgcatgaaac ggactctggt
     3301 gagggcgtta tctttttaac cgatatagcc ggagcgccac cgtaccgcgt ggcttcatta
     3361 ttaagccaca aacactcccg ttgcgaagtg atttctggtg tcacgttacc gttaattgaa
     3421 cagatgatgg cttgccgtga aaccatgacc agttcagcgt ttcgcgagca tattgtcgaa
     3481 ctgggtgcgc cggaggtgag tagtctttgg caccaacaac aaaaaaatcc gcctttcgtc
     3541 ctcaaacata atttgtatga gtattaaccc gcgattctga tgacgctttt gctacaataa
     3601 aagcgttgtt tcaccctcgg ttattttttc atgtacaaac aagctgttat tctcctgctg
     3661 atgctgttta ccgcaagcgt cagtgccgca ttacctgccc attatatgca aaccatcgaa
     3721 aatgccgcgg tctgggcgca aattggtgac aagatggtga cggtggggaa tattcgggcc
     3781 ggacaaatca ttgccgtgga gcccactgcc gcaagttatt acgtatttaa ttttggcttt
     3841 ggcaaagggt ttatcgataa aggccatctc gagccggttc aggggcgaca aaaagttgaa
     3901 gatggtttgg gtgacctcaa caagccgctg agtaatcaga acttaataac ctggaaagat
     3961 acgccggttt ataacgcacc gagtgtgggc agtgcgccat ttggagtact ggcggacaat
     4021 ttgcgctacc cgattttgca taaactgaaa gacaggttaa atcaaacctg gtatcagatc
     4081 cgtattggcg atcgactggc ctatatcagc gcgctggatg cccaacccga taatggcttg
     4141 ccggtgctaa cctatcacca tattttgcgc gacgaagaaa acacccgttt tcgccatact
     4201 tcgacgacca cctcggtacg cgctttcaat aaccagatgg cctggctgcg cgacagggga
     4261 tacgcgacac tgagcatggc gcagctggaa ggttacgtga agaataagat caatctccct
     4321 gcgcgagcgg tggtaattac ctttgatgat ggcctcaagt cggtgagccg ctatgcgtat
     4381 cctgtgttga aacaatatgg tatgaaggcg acggcgttta ttgttacctc gcgcatcaaa
     4441 cgtcacccgc agaaatggaa cccaaaatcg ctgcaattta tgagcgtttc tgaacttaac
     4501 gaaattcgcg atgtatttga tttccagtca catactcatt ttttgcatcg gatagatggt
     4561 tatcggcggc ctatattgct gagccgtagt gagcacaata ttctgtttga ttttgcacgt
     4621 tcacgccgcg ctctggcgca atttaatcca catgttttgt atctttctta tccatttggc
     4681 ggatttaatg acaaagccgt gaaggcagca aaagaagccg gatttcacct ggcggtgacg
     4741 accatgaaag gcaaagtaaa accgggggat aatccgttgt tactaaaacg actttatatc
     4801 ttaagaacgg attcgctgga gacgatgtcg cggctggtga gtaaccagcc gcagggataa
     4861 caatcaggca acctgtaccg gaatcgcttt cgcggtacgt ttcatttcgt tgtcgccttc
     4921 aaaataggcg acgttgggtc gccaggtgcg agcttcttca tctggcatgg taacgaagct
     4981 ggcgatgatg acaatatcgc cgacgctggc gcagtgggcc gccgcaccgt taacagaaat
     5041 aattctcgaa ccgcgttctg ccgcgatggc ataggtggag aaacgcttgc cgttggtgac
     5101 attccagata tcaatggctt cgttttcgag aataccggct gcgtcaagaa aatcctgatc
     5161 aatggcgcaa gaaccttcat agtgcaggtc cgcatgagtc actttcacgc ggtggagttt
     5221 gccctgcagc atcgtgcgaa tcataacttc tacctttcta ccctgtcgtt aacgaagcag
     5281 gcgatgcctg ctttgaggaa attctcacgc agtattgccc gatttttgtt tagtgtctac
     5341 tcatctgacg gcatttgcag cagcagtttg cgtaccgtgc cgaagcgtgc catgtaacgc
     5401 ctgtaaccaa ttgaaattta tctgaacgct gctcggtaaa cactaaagag gcggctgacg
     5461 acggcacacc atgactggat actgccttca cgcaagacca cacggaaggc agggaagatg
     5521 gatgcaccga gtaccacacc gcatgatgcg gtgtttaaac aatttttaat gcatgcggaa
     5581 acggctcgcg actttctgga tatccatttg ccagcggagc tacgcgaact gtgtgacctc
     5641 gacacgctgc atcttgagtc ggggagtttt attgaagaaa gcctgaaagg gcacagcact
     5701 gacgtgcttt attccgtgca aatgcagggc aaccccggtt atttgcatgt tgtgattgaa
     5761 catcaaagca agccggataa gaaaatggcc tttcgcatga tgcgttattc tattgctgcc
     5821 atgcaccggc atctggaggc tgaccacgat aagctgccgc tggtggtgcc aattctgttt
     5881 tatcagggcg aggccacacc ttatccgcta tcaatgtgct ggtttgatat gttttactca
     5941 ccggaactgg cgcgacgcgt ctataacagt cctttcccgc tggtggatat cactatcaca
     6001 ccggatgacg aaatcatgca acatcggcgg attgcgattc tcgaactgct gcaaaaacat
     6061 attcgccaac gcgacttaat gttattgctg gagcaactgg tcacgctgat agacgaaggg
     6121 tacactagcg gaagtcagtt agttgccatg caaaactata tgctgcaacg cggtcatact
     6181 gaacaagcgg atttgtttta tggtgtggtg agagacaggg aaacgggagg ggagtctatg
     6241 atgacgctgg cgcagtggtt tgaagagaag gggattcagc agggaagaca ggaagtaagt
     6301 caggaattcg ccctgcgtct tctgagtaaa ggaatgtctc gggaagacgt tgcagagatg
     6361 gtaaatttac ctcttgctga gattgataaa atgattaact aagttgtctg cattatgaat
     6421 aattgcctac agataacgtg gcaatgaata actggcgcca tcagccgtag cgccagttaa
     6481 gcattacgcc agttcgacca gtttgttgtc gatcaggcga gcatcgccaa gccaggcggc
     6541 taccagaatc accgctcgtt tgctgttttc agagatttcc agcaaggtgt cggcatcgcg
     6601 aatctgaata tcatcggaac ggaaaccttt ttcattcagt tcttgcccgg caatagcaat
     6661 aatttcatcg agatcccgtt cgccagcctg caatttgtcg gcaattgaac ttaagacttt
     6721 gtagagaccc ggcgcaattt tacgttgttc cgccgtcaga tagccgttac gggaactgag
     6781 cgccagaccg tctttcgcgc gcataatcgg cactccgaca atctcaatat cgaagcccat
     6841 atcggcaacc attttgcgga tcagtgccag ttgctgaaaa tccttttcac cgaagcaggc
     6901 gatatctggc tggaccaggt tgaacagctt gctgacaata gtcgaaacgc cgcgaaaatg
     6961 tcccggacgg ctggcacctt ccagcatggt ggaaagaccg ggaacatcaa cataggtgtg
     7021 ggtttcagta ccgttcgggt agatctcttt taccgaaggc gcgaaaacta aatccacttt
     7081 acgtttgttc agcttttcgc agtcctcttg caaggtccgt ggataacgtg ccagatcttc
     7141 cgggcggtca aactgcattg ggttaacgaa aatactgacg acgaccacat cggcgcgggc
     7201 tttggcttcg tcgaccaggt tcatatggcc atcgtgcagg ttacccatgg tgggcaccag
     7261 cgccacgcgc ttaccttcca tacgcaggcg gcgaatttgc tgacgcagca gcggcagggt
     7321 ttcgataatt aacacaacgt cactccttaa tggaaactgt gttcttcgcc cgggtaaacg
     7381 ccggactcca cttcagccat atactgccgc acagccgcgc ggatgtcgcc cgtttcggcg
     7441 aggaaatttt tggcgaattt aggaatatga ccgccggtaa tgccgaacgc gtcgtgcatc
     7501 acgagaattt gcccgtcagt gacgttgcct gcgccaatac cgataaccgg gattgccagt
     7561 gcttcggtaa tgcgttttgc cagctcaaca ggcacgcact ccagtaccag cagttgtgcc
     7621 ccagcagctt ctaaggctaa tgcatcgctg agcagtcgat cgcccgcttc atcgccgcgc
     7681 ccctggactt tgtagccacc gaaaatattc actgactgtg gggttaggcc taaatgacca
     7741 caaactggaa cggcacgttc ggtcagcatt tgtacggttt ccaccagcca ctcaccgcct
     7801 tcaattttga ccatgttggc tccggcacgc attaccgttg cggcgttttc aaaggcttgt
     7861 tccggcgtgg cgtacgccat aaatggcagg tcagccagca gaaggcagtt tggcgcgccg
     7921 cgacgcacag cggcggtgtg ataggcgata tcggcaacgg taaccggtag cgtggagtca
     7981 tgcccctgga ccgtcatgcc cagtgaatcg cctaccagca tgacgttaag tccttcttcg
     8041 gcaaacagtt ttgcgaagct atagtcgtaa gcggtgatgg ttgcgaaacg ttttttgtcc
     8101 tgcttgcatt tctgcaatga agcaatcgtg gttggtttca tagcgtatcc tgataaatga
     8161 tgttgtgctg tctcgcattt tatcagtcac attggtgggg gcaatgattt atccgtatcg
     8221 caccgccgtt gtctggcggt gcgatagtgt tatcaggggt aggtaacctg aaaagtgctg
     8281 gtggctttaa aatcccccgg ttctatagca atattaccgt cttgttttaa tgtagcctgg
     8341 aaatgaagag gttgagaggt cccaccgttg ccattatccg ggaaaatccc cccagtagtg
     8401 tcattttctg tttcataatc tttataaata gacgttgcat catttggttt cagtaccatt
     8461 tgtgcacttt tagtgttttt taaaccttca atcagtacac caacgccttt tgctgcgtca
     8521 tttcctgtga gtgtgttagc caaaagttct ttgcttacac tacccacctt gtttgacttg
     8581 agttttgttt ctatattgcg aacacgaata cagttctgta gcgtgatatc aaaaggaacc
     8641 gctgttgcgc cattttttat ttgtgcaggg ctataatcac ccattttaac cgttgaacca
     8701 cttaccgatg ggccactaag cgcagcagta aaacaggtag ggtttgttaa tacgatatct
     8761 gtcaagtcaa agtcgatttt aagaaaataa ctcttgatac taacgccagc attatatgct
     8821 ttgaatgagt agagataact atctgttctg gataatgtta cccgttgatt cgttgtcggg
     8881 ttaaagtcgg tatcggtata aaattcaatc gtgagcgttt gcataacgcc accaacggcc
     8941 cagtattttt tgctgctatt cgcgttgttg caagaccaat atagggtttg ctcgctttcg
     9001 ccacgccagt taaaagattg gctcgtagaa tcgccaatat acatgcctga tgggttgata
     9061 tcagtagatg taagtgttga ccaaatgttt gatattgcta acgtgtaata caggcctgga
     9121 acagatgtct taaagagttt atgtccgcca taactttttc cggaatcaac cattttatgt
     9181 tcaatagaca ttggtgcgcc gcttgagtta ctactcctgt tacagtaaag gaaacctcca
     9241 cccgaagcgg aatctaactg cgcccatttt aatgacaccc cggttgggcc aagttgggtt
     9301 ggtttataaa aaactaaatt attggtgcct gaaccatacc ggaatttgac ggtaacatct
     9361 ccattgggac ctgtctctcg ccatttttgg tctgatggac caacaccgta attattccca
     9421 acaatatagc cgacgtcatc atcggtgccg gcgcttgctg tataacaaca agagcatagt
     9481 gctaaaaaaa gaaagtatct gaagaatgtc ttcattttta ctccattgtc atgctgcaag
     9541 ataatacttg tagcactttg tctgttattc gtagtagata tccactaaca catggcttat
     9601 cagtgggcca gaggtgacat tcagtgctgg atcgattttt tgcattcggg tactaaattt
     9661 ccaggatgaa ttatttagtg ctgatgacat gaccggataa acggaacgtg agctaccagt
     9721 ggcactaaga acgttgaagg tatttgccgg gtcctgaata gaaaatataa ctaccccaac
     9781 atttttagcc ccggcgttgt cttcattagc gaatatttga ctggaacctg tcgccattac
     9841 gccactaagt gcgcgaaaat taatatgtaa ctgatttaac acattccctg tgccctgaag
     9901 tgtgcagtcc tgtactgtaa tagtgaactc ttttccaccg gggtgataat tttcggctga
     9961 aatgtttccg gtaaaatcgc ccacgccaac gacacctaaa tcaatatttc cgttgttgct
    10021 gattccactt tggcaggtgt tattttttat atttgcaaca atgtcaacat cctgtcctgc
    10081 aaccacaggg gaagtggcgc tgaatagcaa cgacagaatc agaattgatt ttttcatcag
    10141 attacgttga gtgggacgca tatttagttc ctgaatcagt ctgcctgtta gttataagtc
    10201 acggtgaatg ttgccggggc agtgaaatca cccgcagtga catttgtgac agttttatct
    10261 tttgcgacca ccaacaccgc actcattggg taatcgacaa cattgctgcc cgtcgcatta
    10321 gcaatactga cttcttctgt tgcgggggtg acacaactga attgtttagc gccgttctta
    10381 cctggtgtgg atgtgctcca gacctcaacg gcgaccgccg ctgcttttgc tgttgccgtt
    10441 gatgcgttag caaatccagg accattatta gaattacctt cacataatgc tcgtgtagtc
    10501 aattttattt tggccttttt cccgggaatc cccgcacagt ctttgaactg taatttgaag
    10561 gttttaattt tagtttcggc attaatttca gaaatatata catcaccgaa attgatggtg
    10621 gaaattttag ttgtaccgtc atcagtcaac gttgatgtac aggtacccat ttctacgtca
    10681 gtagtaacag tcaggccaat actgtcagtg gccgcatgtg aggaaatggt taacagtgat
    10741 gaagccagaa atagactacc cgataatcca taacataatt tttttaatat catcattgtc
    10801 atccttgtta ttgatagctg aagttaaaag ttgccagtgc gcgaaacttg cctggaacac
    10861 ctttccctac acttgtttca cgcagtgcga cagtcatctc aagtccatcg gtgctaattt
    10921 ctgatgcact ccaacgtata tattctgcgc tattaggttt cagaaatgta tcgcctgaag
    10981 tcgccttcct tttaaatccc attccgatat agcttgtggt ggaggttgta tcactggcca
    11041 gggggataat tagcgcgggt gaacttcctg attgatttcc tgtcagggtt gtatcaatcc
    11101 agctaattcc actgctgcat ccattggcta ccaatttaaa gttggcttca gattgtgctg
    11161 ttttattggc tattttatcc attcccatcg agggaataac taaggtgtac ttatcatttc
    11221 cattatcagt gacattagtt ccggtaagtg taatattaca ggttgttgct ttcacggtag
    11281 cggtaaaatc gacattaaca tccgttgcaa ataccgaagg cgataacaat agtcccatga
    11341 gtattgttat tttatgtggt gtgattttta tcattggaag tcccttgtgg ttactgtatc
    11401 tgacaacgaa gtccgttcag aataatagat tgtgctattt tttctgatgt cgtgttttgt
    11461 tgataatgag caaaacaact gaccggtttg ccctcttcaa tccaggtaat tcgtatattt
    11521 ccttcctgcc cgataccgcg aacaaaggcc tgaccaccct gacctacgtt gccgatgata
    11581 ttgttttgtt catcataaat atccgctgca aatggaatat ttttaccatc gcttcgcacg
    11641 atgttcataa ttgccgattg tccctgaact gtttcaaaat cagcaaagac gacggctccc
    11701 tggcgcggga cggcgacagt actggtgctt ttcaattcaa catcgttttc cagatcgtta
    11761 atgtccagtg cgatacgatt ttcatggtat ggcgaaaggg cgctggtcac accataaccc
    11821 cagcggtcta cggtgctgtt tccgtagtta atacgagcgc cttttgctcc tggcgcctga
    11881 atgacagcca gagtgtcgga atcactaaaa ctgtcattac tgaacgtcag cccgccgcta
    11941 tgtaacacaa atccaccatc agtattgagt gagaattggc gactattatc actgcttgct
    12001 gaggctgaac ctgacagcgt tccccacggc gattcataac tggcataacc gccgatataa
    12061 ctcaaatcat cactggattt attcatcata taacccgtgt ttacgctgta gctgatgcga
    12121 ttggtatcgc tgtaaccact actgctgata ttgagttggt tattaccctt aaagtcgctg
    12181 tttaattgtg tatcaatact ctggaagccg gagctgcggt gttccgtacc cagtaaattt
    12241 tctattggaa tcgtgaagct gagataaatg ctatcgtcgg tttgaccatc ttcattcaac
    12301 gaacgttgag cactgatact gtaactgccc caggacgcac tattgctata accaatagag
    12361 taattagtac tgttttgccc ggaggcccag taatctgacc agcttcccga gagataaaat
    12421 gaaccgtaat cctttttctc aaatttcagc ggttggttga tactgaccgt gacctgattt
    12481 ttcatgcgcg agtaattgcg catggattta ggttcaagct cttgctcagg atgttctact
    12541 tcatcaatca gcgtcagcgc atcattgagg cccagatagt gctgggtgga gtagcgatag
    12601 gccgcgatat ttaatgatgt actggtattt tcaaataact tgttccagga aatacggtag
    12661 ctttgtcctt gataggtttt atcgtccgga atgctgacat ttgagtgagt aacatcgaca
    12721 gaaaaagccc ccacgggggt attcatacca agccccagca atccggcggt gtagttatta
    12781 tcagttagct gaataccggt atatcctgtc aggtagttat tcaaaccata gtagtagctg
    12841 gcttgaaata aattaggctc atcctggata ctgtctttta aaacctgacc ggcgctaata
    12901 tcccagcgac caacgccagg gcgcagcatt tgcacaacag atgaaaatgg ctgggagaac
    12961 gtccgttttg tgccatcagc ttcttcgata gtcacaatga ggtcactgcc ataacctgac
    13021 gggctaagat cgtcaatggc aaaggcccct ggcggcaccg tcgtttcata gattttataa
    13081 ccattttgca taacggttac tttggcattg gtattcgcta cgccatgaat gattggcgca
    13141 aaactggcta ataccggagg cagcattcga ctgtcactgt ataagcgaat accgcgaata
    13201 ctgactgaat cgaaggtttc gccggttgtg taagattcac caataaccag ttgtgaacgt
    13261 agtgacgcaa gatcgcgttg taaatagcga ttctgaaaat cgtaatcgct atggacattg
    13321 gtcatccagt tgtagttacc cgaggcacgt aaccgccagg cacctaagtt tatcccaccg
    13381 ttgaatgccg catagatact gtcatttgtt ctgcctggac tttcactgtg atatccgttg
    13441 aggttgtagg aaagcatggc tgcattaatg ccattttccc acaaagaggg atcaacatag
    13501 ttttgataat tcttcataat ccatgcctgg gggacatcga tatccaggcg ttgatcgtta
    13561 acatcgtaac gaatggatga ttgagggatc atttcagcca gattgaggca atcaccaaga
    13621 tcttcgtctc gcttaagcaa aatggctttt tcgctattct tgtctggtga actgatatga
    13681 aattgcaata aatttttctg cgtaatgcag gcctgagcgt tctttttacc ttcaataaca
    13741 gcaaaagcaa tgctttggct catgatgggt tgatcgttaa catacacact gacatcgtaa
    13801 atgccaggta gtgtgggatt gccttctgta taccgactaa ggtcgatatt tgaagcatcc
    13861 tttcccatca gaaatgta
//
LOCUS       DADSZD010000114        13776 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.115, whole genome shotgun sequence.
ACCESSION   DADSZD010000114 DADSZD010000000
VERSION     DADSZD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.115"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(312..737)
                     /gene="arsC"
                     /locus_tag="J1Z28_003127"
     CDS             complement(312..737)
                     /gene="arsC"
                     /locus_tag="J1Z28_003127"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBA7717939.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFTKEDGEKVVDEAGNRLK"
     gene            1271..1354
                     /locus_tag="J1Z28_003128"
     CDS             1271..1354
                     /locus_tag="J1Z28_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBA7717940.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1408..2760)
                     /gene="gorA"
                     /locus_tag="J1Z28_003129"
     CDS             complement(1408..2760)
                     /gene="gorA"
                     /locus_tag="J1Z28_003129"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBA7717941.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2832..3674)
                     /locus_tag="J1Z28_003130"
     CDS             complement(2832..3674)
                     /locus_tag="J1Z28_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HBA7717942.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3877..5919
                     /gene="prlC"
                     /locus_tag="J1Z28_003131"
     CDS             3877..5919
                     /gene="prlC"
                     /locus_tag="J1Z28_003131"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBA7717943.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENDELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            5927..6679
                     /gene="rsmJ"
                     /locus_tag="J1Z28_003132"
     CDS             5927..6679
                     /gene="rsmJ"
                     /locus_tag="J1Z28_003132"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBA7717944.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6728..8197)
                     /gene="dtpB"
                     /locus_tag="J1Z28_003133"
     CDS             complement(6728..8197)
                     /gene="dtpB"
                     /locus_tag="J1Z28_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBA7717945.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8514..8948)
                     /gene="uspA"
                     /locus_tag="J1Z28_003134"
     CDS             complement(8514..8948)
                     /gene="uspA"
                     /locus_tag="J1Z28_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBA7717946.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9339..9674
                     /gene="uspB"
                     /locus_tag="J1Z28_003135"
     CDS             9339..9674
                     /gene="uspB"
                     /locus_tag="J1Z28_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBA7717947.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9745..11244)
                     /gene="pitA"
                     /locus_tag="J1Z28_003136"
     CDS             complement(9745..11244)
                     /gene="pitA"
                     /locus_tag="J1Z28_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBA7717948.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTIDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11476..12669
                     /locus_tag="J1Z28_003137"
     CDS             11476..12669
                     /locus_tag="J1Z28_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBA7717949.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGITWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(12756..13763)
                     /locus_tag="J1Z28_003138"
     CDS             complement(12756..13763)
                     /locus_tag="J1Z28_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002432832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HBA7717950.1"
                     /translation="MGIICIALGEFVLTGSGQSEYFVAGHVLISLSAICLALFTTAFI
                     IISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMADEFVAGHVIFGVGMI
                     AACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLIAVPVLLTLFGFIWSV
                     ILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTFSVKEHWLWCYWVILL
                     GSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWLLALVWRRTCSLANRI
                     PMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCFTLFSIVSILEAGSAK
                     K"
BASE COUNT         3517 a         3492 c         3619 g         3148 t
ORIGIN      
        1 gtatgttgaa attatttatt gtgtaaacca ctgatagatc ataatccagt acatctttac
       61 agtgctgcac tgtatcgctg ataactaagc agctcacaga tacaccatgc tggacgcgat
      121 aattggaatc tcacgcaggg agtactgtgt aaatagcaaa attgcctgat gcgctacgac
      181 tgtcaggcct acaaataaca tacaattcat cgaattatca taactttgta ggtcggataa
      241 aacgcgccag cgtcgcatcc gacataaata aagcgcattt ttctaacaac ctgtggggga
      301 atatagccgc tttatttcag gcgattaccc gcctcatcaa ccactttctc gccatcttcc
      361 ttggtgaacg cgcctttttg cgcatctggc agaatttcca gcacgacttc tgaagggcgg
      421 cacaggcgag ttcccagcgg cgtcaccaca atcgggcgat taatcagaat cgggtgctga
      481 agcataaagt cgattaactg atcgtgagta aatttatctt ctgcaaggcc cagttcctca
      541 tacggctcga cgtttttacg cagcaacgcg cgtacggtaa tccccatatc ggcaatgagt
      601 tttaccagct catcgcgcgt cggtggtgtt tccagataat agataatggt cggttctgta
      661 ccgctgttgc ggatcatctc cagcgtatta cgcgacgtac cgcaggccgg gttgtgataa
      721 atggtaatgt tgctcatatc agtatctcat tacgatgtga aagagaggtg tagcgccagc
      781 gccaccagag ttacaaacca aagcggacaa gtcaaaaaca catatggttt aacgaatatg
      841 taagtgtaga gaaaggtgta ggtgtgaatt aagcgttggg cattcatttg aaaggaggtc
      901 tgaatcagga cgtaaaaaaa ccgacttcgc gtcggttttt ttactgttca gccctgagtt
      961 ggtggctctg gctggagtga gatagccatc atctaacgca tcaatgctaa taccatactg
     1021 aaactattgc aaggacatgc tggttttata acccgcaagt actgtatgat tatccagtta
     1081 gctctgagac attttcactc tggcaatgcg cataaacgct ttcaaagtcc tggtctgaag
     1141 tacgggtggt gccgttaact gatgctctgg ccggagtgag atagtcgtca tctaacaatg
     1201 agacatgcgc cgtgacaggc aatggatgag taagcggatg cattctcact ccatcgcatg
     1261 gagaaaacgg gtgattgata aagcaatcat cgttcttggg gcgttaattg cgctgctgga
     1321 actgatccgc tttctgcttc agcttctgaa ctgatagcgg aaacgtagct aagggctaag
     1381 agcacactac tcttagccct ttaacattta acgcattgtc acgaactctt ctgccgctgt
     1441 cgggtgaatg gcaaccgtgt tgtcgaagtc ttttttggtt gcacccatct tcagcgccac
     1501 cgcgaagccc tgcaacattt cgtccatacc aaaaccaatg ccgtgaatac cgacaatctt
     1561 ctcttccggt ccaacgcata ccagtttcat gcggcacggc tggcggtgag tggtgacggc
     1621 ggtatacatc gcggtgaaag aggatttata cactttcacc tgatcgtcgc cgtactgctc
     1681 gcgcgcctgc ggctccgtta aaccaacagt accaatcggc ggatggctga agaccacggt
     1741 cggaatgttg ctgtaatcca gatgctcatc cggcttgtta ttaaacaggc gttcagagag
     1801 acggcgacct gctgcaacag ccaccggtgt cagttccact gcacccgtgt tatcgcccac
     1861 cgcgtaaata ccttcaacgt tggtgttttg atatttatcg acgacgatat aacctttttc
     1921 gttagtttta acgccagcgg cttccaggtt gatgttgtca ttggcaggct cgcgaccaat
     1981 cgcccaaatc aggcaatcca ccgtttcact gcgaccatct tccagctcca gcgtcaggct
     2041 accatcggca tttttcacta ccgctttcgg gatggcgttg gtgtgcaact gcgggccttc
     2101 ggcgttcatc acttcaacca gcgtttcaga gatcatcggg tcgaagctgc gcagcggcgc
     2161 atgtttacgc acaaacagat gcgtcttcgc accgaggccg ttaatcacgc ccgccagttc
     2221 aacggcgatg taacccgcgc caacaaccgc tacacgctct ggcaaagccg gaagggcgaa
     2281 gaagccatca gaatcaatac cgtattccac gcctggaata tccgggtggc tcggacgacc
     2341 accggtggcg atcagaatat gatcggccgt gatggtttcg ccgtttacct ccagcgtttt
     2401 ggcatcaacg aaacgggcaa agcctttgat tacatcaacg ttatttttac cgagcacgtt
     2461 ctcataggaa gtatgaatac ggtcgatata ggcggtacgg ctggcgatca acgtttccca
     2521 gttgaattta ttgatagtgg tatcaaaacc ataatccggg ccgtacatat ggatcgcttc
     2581 acggatttgc gccgcgtgcc acatcacttt tttcggcaca cagccaacat ttacgcaggt
     2641 gccgcccagc tctttggctt caatcagtgc gcatttctgg ccgtacatag ccgcgcggtt
     2701 gatggaggcg ataccgccgc tgccgccgcc gatggcgatg taatcatagt gtttagtcat
     2761 gacatattgt ccttatcgtt gattaccgcg attgtagcgc gagacgtaat aggtgccagc
     2821 aatggctgca attactctgg cacgatccag cttacggtgg cgtgcccggt gcctgccgga
     2881 accagtttgc tgtgcagcca cggcagcacg ttattcatct gctgttccag cttccacggc
     2941 gggttaatca caatcatgcc ggaagcggtc atgccacggc gatcgctgtc tggcagcacc
     3001 gccagttcaa tttgcagaat tttgcgaatg ccggtcgctt ccaggtcgtg gatcatgcgc
     3061 ttaatttgct ggcgcagcac cactggatac cacaatgcgt aagtgccagt ggcgaaacgt
     3121 ttgtaacctt ctgctatccc gctgaccacc gcctgatagt cggttttcat ttcatacggc
     3181 gggtcgataa ggattaaacc acggcgggaa accggcggca gcttggcctt aagctgctgg
     3241 aaaccgtcgg ctttttcgac gcgcgcacgg ctgtctttct gaaattcaga acgcagcaac
     3301 gggtaatcgc tcgggtgcag ttcggtcagt tgcaggctgt cctgttcacg cagtagctgg
     3361 cgagcaatca acggcgaacc ggggtaataa cgcaactgcc cgctacggtt gaagtgtttt
     3421 accacattga tgtacgcctc cagctccgca ggcaaatcgt cctgctgcca gatacgggcg
     3481 atgccttcga gatattcgcc ggtacgctcg gcatgttcgc tgcctaactg ataacgcccg
     3541 gcccctgcgt gggtgtcgag atagagaaac ggtttatctt tctctttcag cgactcaatg
     3601 atcaggctct gaacggtatg tttaaggacg tcggcgtggt tgccagcgtg aaagctgtgg
     3661 cgataactga gcatgggtaa aggtgttccg gtaagtaaaa atcggcccgt attcggggcg
     3721 cacaaaagcc tatcaggaca gtataccgaa aagaggccgc cgccgcgaaa gcgtaacgtt
     3781 tctcattgaa attcactaca cttaacccca tgctacaaac attatgtaaa gcgcctgttg
     3841 agcgcttcct taacctcttt aaccaggact gcgctaatga cgaatccgtt actgactccc
     3901 tttgaattgc ctccgttttc taaaattctc ccggaacatg tcgttccagc cgtgactaag
     3961 gcgctgaacg actgccgcga aaatgtggag cgcgtagtag cgcaaggggc accgtacacc
     4021 tgggaaaatc tctgccagcc gttggcggaa gtggacgatg tgctggggcg tatcttctcc
     4081 ccggtcagcc atctgaactc ggtgaaaaat agcccggaac tgcgtgaagc gtacgaacaa
     4141 accctgccgc tgctgtcaga atacagcacc tgggtagggc aacatgaagg gctgtataag
     4201 gcatatcgcg acctgcgcga tggcgatcat tacgccacgc tgaacacggc gcagaaaaaa
     4261 gcggttgata acgcactgcg cgacttcgaa ctctctggca tcggtctgcc gaaagagaaa
     4321 cagcagcgtt acggcgaaat tgctacccgt ctttctgaac tgggcaacca gtacagcaac
     4381 aacgtcctcg atgcgacgat gggctggacc aaactcgtta ccgacgaagc ggagctggcg
     4441 gggatgccag aaagcgcgct ggctgcggca aaagcccagg ctgaagcgaa agagctggaa
     4501 ggttatttgc tgacgctgga tatcccaagc tacctgccgg taatgaccta ctgcgacaac
     4561 caggccctgc gcgaagagat gtatcgcgct tatagcactc gtgcttccga tcaaggtccg
     4621 aacgccggta agtgggacaa cagcaaggtg atggaagaga tcctcgcgct gcgtcacgaa
     4681 ctggcgcaac tgctgggctt tgaaaactac gccttcaaat cacttgccac taaaatggca
     4741 gaaaacccgc agcaggtgct ggatttctta accgatctgg caaaacgcgc gcgcccgcaa
     4801 ggcgaaaaag agctggcgca actgcgcgcc ttcgccaaag ccgaatttgg cgtcgatgag
     4861 ttacaaccgt gggatatcgc ttactacagc gaaaaacaga aacagcacct ctacagcatc
     4921 agcgatgaac aactgcgtcc gtacttcccg gaaaacaaag cggttaacgg cctgtttgaa
     4981 gtggtgaaac gtatttacgg catcaccgct aaagagcgta aagatgttga tgtctggcat
     5041 ccggatgtac gtttcttcga gctgtatgac gagaacgacg aactgcgcgg cagcttctac
     5101 ctcgacctgt atgcccgtga aaacaaacgc ggcggggcgt ggatggatga ctgcgtaggc
     5161 cagatgcgta aagccgacgg ttcgctgcaa aaaccggtcg cgtatctgac ctgcaacttc
     5221 aaccgcccgg taaatggtaa accggcgctg tttacccatg acgaagtgat caccctgttc
     5281 cacgagttcg gtcacggcct gcaccatatg ctgacccgca ttgaaaccgc tggtgtttcc
     5341 gggatcagtg gggtgccgtg ggatgcggtc gaactgccga gccagtttat ggaaaactgg
     5401 tgctgggagc cggaggcgct ggcgtttatc tccggtcact atgaaaccgg cgagccgctg
     5461 ccgaaggagc tgctggataa aatgctggca gcgaaaaatt accaggcggc gctgtttatt
     5521 ctgcgccagc tggagttcgg cctgttcgat ttccgcctcc atgccgagtt ccgtccggat
     5581 cagggggcga aaatcctcga aactctggca gaaatcaaga aactggttgc cgtggtaccg
     5641 tctccgtcat ggggccgttt cccgcatgct ttcagccaca tttttgccgg tggttatgcg
     5701 gcaggttact acagctacct gtgggccgat gtgctggcgg cagatgcctt ctcgcgtttt
     5761 gaggaagagg gcattttcaa ccgtgaaact gggcagtcgt tcctcgacaa cattctgagc
     5821 cgtggcggtt cagaagagcc gatggagctg ttcaaacgct tccgtggtcg tgaaccacag
     5881 ctggatgcga tgctggagca ttacggcatt aagggctgat cattcagtga aaatctgctt
     5941 aattgatgaa acaggcgccg gagacggcgc cttatctgtt ctggcggccc gctgggggct
     6001 ggaacacgat gaagacaacc tgatggcgct ggtgttaacg ccggagcatc tggaactgcg
     6061 caagcgtgat gagccgaaac ttggcggcat ctttgttgat tttgttggcg gagcgatggc
     6121 gcaccgacgc aaattcggcg gtggtcgcgg agaggcggtg gcgaaagcgg tgggcattaa
     6181 aggcgattat ttgccggatg tggtggacgc caccgcagga ttagggcgcg atgcctttgt
     6241 gctggcttcg gtcggctgcc gcgtgcggat gctggagcgc aatccggtgg ttgccgcgtt
     6301 gctcgacgat ggtctggcgc gtggttatgc ggatgcggaa atcggcggct ggttacagga
     6361 acgtttgcag ttgattcacg cctccagtct gacggcgctg agcgatatca ccccgcgtcc
     6421 gcaggtagtt tatcttgacc caatgttccc gcataagcag aaaagcgcgc tggtgaaaaa
     6481 agagatgcgc gtgtttcagt cgctggtggg gccggatctt gatgccgatg gattgttgga
     6541 gcctgcgcgg ttgctggcaa ccaaacgcgt ggtggtgaag cgtccggact atgcgccgcc
     6601 gctggcgaat gtcgccacgc caaacgcggt agtcactaaa gggcatcgct ttgatattta
     6661 tgccggaacg ccggtgtaaa agtaaagcgg ggtagcgata acgctacccc gctttctgca
     6721 agaataatta atggctttcc ggcgtcgcaa tcatgcgttt cagccacggc accatcaata
     6781 gcatcactac tgcaacgccc agcgtaacca ggccaatctt accaaacacg ttggtataga
     6841 cgggcaacgt ctcaagtgga tcggtaatgt tgtctggtac tgcggtaaat gttgccacat
     6901 agccgcccag caagaacgcg gcagcctgcg tcaggaacca catcccgaga ataaagccca
     6961 tcaaatgctg cggcaccagg gcagcaatca tcgccaggcc aagggcgcta ataaacagtt
     7021 cacctaagct ctggaataag tacaccagca cgataaacca tggcgatgtc agcccctggg
     7081 catccgcaaa ccacattcca gccgctgcag ccgtcaaaaa gcccagtgag cacataaaca
     7141 tgccgagagt aaacttcatc ggcatcgaga gatctttgcc tttgttaccc agatgcgtgt
     7201 aaatgcctgc cagtattggg ctggcgagta ccacccagaa cgggttaagc gcctggaagc
     7261 tgaccgggtt gatggaaaag ccgagaattt catgatgcac gttgttgatg gcaaagaagt
     7321 tcagcgacgt tggcatctgg gcgtagagaa tgtaaaacac caccgcttcg agcatcagga
     7381 caaaggcgac aaacatttta ttgcgcccgg ttttatccag cttgaatgcc tgacgaaaga
     7441 agatgatggt gacgacgatg gagagaacaa tcagaaccag attggcgact tctacgttgt
     7501 gcatcagcca ggcgcagacg aagatcatca ccacgctgcc aagtaatacg tacaacagtt
     7561 tactgaagct catcggccgg aagtcgggtt cagaaccaat gtctttcacc attccacgac
     7621 aggcgatgta aaccagtaat gcgataatta accctgcacc gcacaggttg taggtgactg
     7681 aatagccgaa tttatcagcg atcacagggg ccagcgataa cgctatcaac gagccgatat
     7741 tgatcgacat atagaacagg gtgaatgcgc catcaagccg cggatctttc ggcgggtagc
     7801 acttcgaaag caagctggct gggttagctt taaacaggcc gttaccgaca gcgatagtcc
     7861 ccagggcgat gaaaatcagg tcaggcttaa gtagcgacat gccggtcatg aagtagccaa
     7921 tcgccagcac aagtgctccg agaacaatgg tgcgtttggt tcccagcagg tggtcgccaa
     7981 catagccacc aatggaaatg aggccataga ccagcgcagc aaaagcacca aaagtgacaa
     8041 aagcctgctc ttgcgagaac ccaagctgtt taacgaagaa aaccgccagt acgccctgca
     8101 cgccgtagta gccgaatcgc tcccataact cgacaaaaaa gatcatgaaa aatgggcgag
     8161 gttgctgcag catccccatg ggtgttgttg tattcatatt tattaacctt ccaataccat
     8221 cctgaaaagt attaacgcgt aagggataac gcgcgaaaaa gtttacatct gtcagagatt
     8281 cttaatattg atgcggagaa tagattaata cactattaca acagaaaata accagctgat
     8341 cacactaatg atagaaaata tgatggtgtt aaatgttgta ttggcagggg ttttgattgc
     8401 gtacgtagac cgcaggccag ataaggcgtt tacgctgcat caggtaacaa ggcgcgccaa
     8461 aaagcaaaac gcccgcatat gcgggcgttc aggaacacgt cgtggaggga agattattct
     8521 tcttcgtcgc gcagcggaac aatcagcata tcaacgtgaa cggtgttgat cagctgacgt
     8581 gcggaagaca tcagtttgct ccagaagtcc tggtggtgac cacaaaccac cagatccata
     8641 tcgtatttct tgattgcatc gaccagtacc tggcccagat cgccgctgcc gctcagggtt
     8701 tcagtgattg ggtagcctgc attggttgaa agctcggtca gtgcatgatg tgtctcttca
     8761 gagatgcgtt tctgcatatc acccagattc acatcgataa gcccggtgta taggtcagag
     8821 tagtttacat ctacgtggat cagagaaact ttcgcattgt aggggcgagc catagagact
     8881 gctttctcta ccagaacttt gctttccggg gagaggtcga ccgcgatgag aatgtgttta
     8941 taagccatag tgttactcct tccataaagt tgtcgatgac tggccagcta gcgtttcttg
     9001 tgccgttacg tcacccgcgt cctgcgaact acgtgcgcgg ggctaaccca gccgtaaaga
     9061 ttcagtgtta agaccatcct taccttatag cgacccggat gatccgtcaa tccgccttgc
     9121 ttactcgtta gataaacaaa ttttcgacat gaatatattg atagtggtta accttctgga
     9181 aaaaaaacaa cctgatctcc tacactatct atagagccgc tcggatgttg gtcaccacaa
     9241 aagcggtttc tccaggacct ggtgttaact gattggcagc gtatcgggag cggtagcccc
     9301 ggggtggaac tccgcgggca ggtcgccggg gaggaggtat gataagcacc gtcgcattat
     9361 tttgggcttt atgtgtcgtt tgcattgtta acatggcgcg ctatttctca tcactacgcg
     9421 cgttgttagt ggtactgcgt aactgcgatc cattgctcta tcaatatgtt gatggagggg
     9481 gcttttttac ctcacatggc caacccaaca aacaggtgcg tctcgtttgg tatatctatg
     9541 cccaacgtta tcgcgatcat cacgatgatg agtttattcg ccgctgtgag cgggtgcgtc
     9601 ggcagtttat tctgaccagc gcattgtgtg gtctggtggt ggtcagcctg attgcattga
     9661 tgatttggca ttaagcactt cagcaataat gacgcataaa aaaagcgggc cagtaaactg
     9721 acccgctcgt tttggtgcgt acgattacag gaactgcaag gagagccagt atagcccgcc
     9781 ggaaagcagt accgcagccg gaagggtaaa cacccaggcc atcagaatac tggttacggt
     9841 tttacgctgt aagccgccgc catctaccac catcgtcccc gcgacagaag aggagagtac
     9901 gtgagtagtg gaaaccggca tcccggtata acttgccagg ccgatagaca ctgccgccgt
     9961 catctgggca gacatcccct gagcgtaggt catgcctttc ttaccgattt tctcaccgat
    10021 agtcgttgcc acacgacgcc agccaatcat cgtaccgata cctaacgcca gcgcgaccgc
    10081 catgatgatc cacaccggcg catactcgat ggtgctaagc atgtcggact tcagtttctt
    10141 caacaggcgc tgatcgtcag cactcacacc aggcatcttc accactttgt cgatagtgtc
    10201 agaaacgcac agcataatgc ggcgcatctg gctacgttga tcaagcgaca gcttgtcgta
    10261 gctttccaca tcgatagtca acatgccttt caggcggttg agcgcgttaa tggtattcga
    10321 cggatggcaa tggaactccg cgggttgcgt tgcgccagct tccggagccg gtactaactg
    10381 atcagcaccg gtcgcttgtt tcagcagtgc aggatgctgc tcaaagtaag cttcgacgtt
    10441 gttgatggca tcacgggtac gggtgatttc gtagccagtg gcattcatgt tcaccacgaa
    10501 gcctgctggt gcgacgccaa tcaataccaa cataaccaga ccaatgcctt tctgaccatc
    10561 gttcgcgccg tgcgaaaacg ccacgccgat agcggaaagg atcagcgcaa tacgcgtcca
    10621 gaacggcggc tttttcttgc cgtctttctt ttcacgctcc gctggggtca ggtggatacg
    10681 ggcgcgtttc ttggtgccgc tccagtagcg acgcagcaag aaaatcagac cgccagcaaa
    10741 caccaggccg acaatagggg aaacgatcag ggaaccgaaa atacttaata ctttcgggat
    10801 attgagtgca tccaccactg acgtcccggt catcaacgca ttggttaaac caatcccgat
    10861 gatcgcgcca atcagcgtat gagagctgga tgcaggtaaa ccaaagtacc aggtacccag
    10921 gttccagata atagccgcca gcaacataga gaacaccatg gcaaggccat gagacgatcc
    10981 catattaagc agcagatccg tcggcagcat atgcacaatg gcataggcaa cactcagacc
    11041 acccagcaaa acacccaaaa agttgaacac cgccgccata accacggcga gctgagaacg
    11101 catcgcgcgg gtatagataa cggttgccac ggcgttggct gtgtcatgga aaccattgat
    11161 ggcttcgtag aacagcacaa aagccagtgc aagcaataat aacagcccgg tatgcaaatc
    11221 caggccagca aacaaatgta gcataggacg ttacgccatt ttgaggacat gaacgcggcg
    11281 cattatcagt gactttcgcg gcgcgggcaa agtgaaatat agactttttt tgatttgcct
    11341 cctgtatgga ttccactcaa aaaataatta tcttatataa ttcaggcaaa tacttccttt
    11401 tagtaatatt gatgctggtg cgaccactga ggaatcttta caattcacgc ccgttttttc
    11461 ttagaggagc gcaacgtgga aaggtttgat gccattatta taggcgctgg tgcggcaggt
    11521 atgttctgtt ctgcgctggc aggtcaggca ggacgccggg ttctgctgat cgataatggt
    11581 aaaaaaccag ggcgcaaaat cctcatgtct ggcggtgggc gctgcaactt taccaacctt
    11641 tatgtcgaac cgggcgctta tctgagccag aatccgcatt tttgtaagtc tgcactcgcg
    11701 cgttttaccc agtgggattt cattgatctg gtcaataaac acggcatcac ctggcacgag
    11761 aaaacgttag ggcaactctt ctgcgatgac tccgcgcagc agattgtcga catgctggtg
    11821 gatgagtgcg agaagggcaa tgtgaccttc agattgcgta gcgaagtgct gagtgtggcg
    11881 aaggatgata caggcttcac gcttgaactg aacggcatga ctgtcggttg cgaaaagctg
    11941 gtcattgcga ccggtggact gtcaatgccg gggctgggcg catcgccgtt tggttataag
    12001 attgccgaac aattcggcct caacgtgctg ccgacccgtg cgggtctggt gccattcact
    12061 ctgcataaac cgttgcttga agagttacag gtgctggcgg gcgtggcggt gccttccgtg
    12121 attaccgctg aaaacggtac cgttttccgt gagaacttac tcttcaccca ccgcggctta
    12181 tctggaccgg cggtgttgca gatttccagc tactggcaac cgggtgaatt tgtcagcatc
    12241 aatctgctac cggatgtgga cctcgaaacc ttcctgaatg agcagcgtaa tgcacatccg
    12301 aatcaaagcc tgaaaaacac gctggcggtt catctgccga agcggttggt tgaacggtta
    12361 caacaactcg ggcaaatccc ggatgtctcg ctaaaacagc tcaacgtgcg tgaccaacag
    12421 acactgatta gcacattgac tgactggcgc gtacaaccca acggcactga aggctatcgc
    12481 actgccgaag tgacgctcgg cggcgtggac accaacgaac tctcttcacg gacgatggaa
    12541 gcgcgcaaag tgcctgggct gtacttcatc ggcgaagtga tggacgtcac cggctggctg
    12601 gggggctata acttccagtg ggcgtggtcg agtgcatggg cttgtgcgca ggatttggtg
    12661 gcagaataag aagcaaaatg tgctctcgat accagaggga ttaacctgaa taaaaccaga
    12721 taaggcgtcc aacgccttat ctgaagcctt ttttattatt ttttcgctga acccgcttct
    12781 aatatagaaa cgatggaaaa cagtgtaaaa caaactgcac ctaagccaac cagaactcgc
    12841 gaaggaataa agtaagccat atcggtttgc gcaatttcag ccaggaacgc agcaaggaac
    12901 agacaaaaca gacaggtaaa taccggaatc atgggtattc tgttggctaa cgaacaggtg
    12961 cgtcgccata ccagcgctaa caaccacact ttggagaaaa tactgtagca gatcatcccc
    13021 aggcaaatga gaataatgcc gggggccagg cgggcgcttg catctgaact gattaatacg
    13081 tagataccaa aaataatggt aagtgagcca agcaaaataa cccaatagca ccacagccaa
    13141 tgttctttca ctgagaacgt attccgcgtt tgatggacaa tagtggcgac aaggccaata
    13201 agacaagcgc aaattgccgt taaccccatt agtacgtgac ctgcaacata atgtggtgtg
    13261 atatctgcac tacgtaacag tatgactgac caaatgaaac caaagagagt aagcagaacg
    13321 ggaacggcaa ttaagcaatt accgattgtt gaagaatagg cctgcaatgg cgtaccgtca
    13381 ctcttactgc ccgaggcgtt tttggggatc aacagaaaat gtccggaaga tgccgctacg
    13441 gttgatacac aagcggcaat catgccaacg ccaaaaataa catgaccggc gacaaattca
    13501 tctgccatca cattattgct tgccaacagt gaccatcccc aaatcatagt tgtagcagaa
    13561 ccagcataac caataacagg aaataacctg ttgtagaatt tattcatgcc gtgagttaac
    13621 tgagatataa taataaatgc ggtagtgaac aatgccagac atatcgcaga aagagaaatc
    13681 agaacatgcc ctgcaacgaa atattcactt tgcccagaac cggttaatac aaactcacct
    13741 aatgcgatgc atattatacc catcagtaaa ggaatt
//
LOCUS       DADSZD010000115        13733 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.116, whole genome shotgun sequence.
ACCESSION   DADSZD010000115 DADSZD010000000
VERSION     DADSZD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.116"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(171..923)
                     /gene="yecC"
                     /locus_tag="J1Z28_003139"
     CDS             complement(171..923)
                     /gene="yecC"
                     /locus_tag="J1Z28_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBA7717951.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(920..1588)
                     /gene="tcyL"
                     /locus_tag="J1Z28_003140"
     CDS             complement(920..1588)
                     /gene="tcyL"
                     /locus_tag="J1Z28_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBA7717952.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1603..2589)
                     /gene="dcyD"
                     /locus_tag="J1Z28_003141"
     CDS             complement(1603..2589)
                     /gene="dcyD"
                     /locus_tag="J1Z28_003141"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBA7717953.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2694..3494)
                     /gene="tcyJ"
                     /locus_tag="J1Z28_003142"
     CDS             complement(2694..3494)
                     /gene="tcyJ"
                     /locus_tag="J1Z28_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7717954.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3582..4133)
                     /gene="fliZ"
                     /locus_tag="J1Z28_003143"
     CDS             complement(3582..4133)
                     /gene="fliZ"
                     /locus_tag="J1Z28_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBA7717955.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(4179..4898)
                     /locus_tag="J1Z28_003144"
     CDS             complement(4179..4898)
                     /locus_tag="J1Z28_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBA7717956.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5064..6716)
                     /locus_tag="J1Z28_003145"
     CDS             complement(5064..6716)
                     /locus_tag="J1Z28_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HBA7717957.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            6982..8388
                     /gene="fliD"
                     /locus_tag="J1Z28_003146"
     CDS             6982..8388
                     /gene="fliD"
                     /locus_tag="J1Z28_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBA7717958.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQNSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            8413..8823
                     /gene="fliS"
                     /locus_tag="J1Z28_003147"
     CDS             8413..8823
                     /gene="fliS"
                     /locus_tag="J1Z28_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBA7717959.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEKSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8823..9188
                     /gene="fliT"
                     /locus_tag="J1Z28_003148"
     CDS             8823..9188
                     /gene="fliT"
                     /locus_tag="J1Z28_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBA7717960.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9266..10753
                     /gene="amyA"
                     /locus_tag="J1Z28_003149"
     CDS             9266..10753
                     /gene="amyA"
                     /locus_tag="J1Z28_003149"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBA7717961.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10787..11200)
                     /locus_tag="J1Z28_003150"
     CDS             complement(10787..11200)
                     /locus_tag="J1Z28_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7717962.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11387..12592
                     /gene="yedE"
                     /locus_tag="J1Z28_003151"
     CDS             11387..12592
                     /gene="yedE"
                     /locus_tag="J1Z28_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBA7717963.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            12589..12822
                     /gene="yedF"
                     /locus_tag="J1Z28_003152"
     CDS             12589..12822
                     /gene="yedF"
                     /locus_tag="J1Z28_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBA7717964.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12931..13599
                     /locus_tag="J1Z28_003153"
     CDS             12931..13599
                     /locus_tag="J1Z28_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBA7717965.1"
                     /translation="MCGRFAQSQTREDYLAFLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFIAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPRVLSPEAAREWMRQEVGDKEASEIAASGCVPANQFTWHPVSCAVGNVKNQGAEL
                     IQPV"
BASE COUNT         3371 a         3559 c         3388 g         3415 t
ORIGIN      
        1 ataaataaga tttatatata atttatatta ttcaggcaat gaattacttt tgcaagccat
       61 cgcattctct tatgttatta atgagttatg ctgatttgtt aagcagtttt atcacgtttg
      121 aaatggcgtc ccgccccgac tggtgaatcg tcggggctga ttttttctta ttattgcagc
      181 agaaacttct ccaggaactg gcgggtgcga ggctgctgag gatcagcaaa taacgctttt
      241 gctaccccct gctcgactat ccgcccctgg tccataaaaa tcgcccggtc cgcaacatcc
      301 cgggcaaagc tcatttcgtg cgtcacaatc accatcgtgc gcttttcctg cgccagctga
      361 cgaatggtgt tcaggacttc gcccaccagc tctggatcca gcgccgacgt tggctcatca
      421 aacaaaatca cttcaggacg cattgccaga gcacgcgcaa tcgcaacgcg ctgctgttga
      481 ccgccagaca aacgacgtgg ataactggtt tctttaccgg ccagcccaac tttcgccagc
      541 agctcgcgag cgcgcgccgt ggcctcttct ttcggttcgc ctttgacgat caccggccct
      601 tcaataatgt tttccagcac cgtacgatgc ggaaacagat taaagttctg gaagacaaac
      661 ccgacgtgct gacgcaactg gcgaatcagc gatttttgct gacttaatga acgtgcagta
      721 tcaatagtga tatcgccaac ggtgatggtt cccgcttccg gttgttccag cagatttatg
      781 ctgcgtagca acgtggtttt gccggaacca ctcggaccga taatagccac tacttcgcca
      841 ggcttcacct caaggtcgat accatgcagc accgtctgac cgtggaattt tttcaccagg
      901 ttcttaactt caatggcact catttgggtt ccctctcctg gcgattaagt tgattctcaa
      961 aatggttctg caacgtcgat aacacagtcg ccataatcca gtagatgagc gaagccgcca
     1021 ggtacatggt gaacacctcc agcgtgcgcg aggtaatcaa ctgcgcctga cggaacagct
     1081 ccggcacctg gattgtcgcg gccagcgacg tatcttttac caggctgata aagctgttcg
     1141 acagcggcgg caacgccacc cgcgccgcct gtggcaaaat cgcgcgacgc atggtctgcc
     1201 atggcgtcat accaatactg gctgcagctt cccactgacc tttatcaata gaagaaatgg
     1261 cggcgcgcag cgtttcggca gcataggcgg cagtgttcag cgacagacca atcattgctg
     1321 acggaatggg atctagttcg atgccaaact gcggcaagcc gtaatagatc ataaacagtt
     1381 gggcgatgag cggcgtacca cgaaaaatag agatataaaa acgcgccagc cagcgcacgg
     1441 gccagattgg cgaaaggcgc atcagcgcga gaataaaccc tagcagtaag ccaaaaaaca
     1501 tgccgccgat actgagttgc agcgtatacc cagccccttt cagcaggaac ggcaaggaat
     1561 caataaccag ttgtatactt tcttgcatga gcgtttccgg agctaaacgt ggggatgata
     1621 ggcgaacagc gcaggcgcac cgccggtatg aataaacaga atcggccctt catctttgaa
     1681 gcgtttctga ctgataccgt caatcagccc cgccatcgct tttccggtat acacaggatc
     1741 aagcagaatg ccttccagcc gcgccagcaa tttcactgct tccatgcctt cgtcgttcgg
     1801 cacgccgtag ccaggtgcaa aatagtcatc ccagagtaaa atttccgctg atgcggtcag
     1861 ctccagttct ttcgcaatcg cctgttggag gttaaccact ttcggcaatt gatcggcaac
     1921 agaacgcgac acagtcacgc caatcagttc gctttcaggc atcaggtgtt ccagcccaac
     1981 agccagtccg gcgtgagttc cggcactgcc cgatgcgacc accaccgacg aaatattaac
     2041 tgccccttca cactgttgcg cgatttccag cgcactctcc acataaccca gcgcgcccaa
     2101 agcattagaa ccgccaaccg gaatgacata cgggcgaaag ccttgtgctt cgactcgcgt
     2161 cgccagctct tccagttggg tattgggatc ggtcagtgcg tcgcacattt caatctgggt
     2221 attgaacaga tccagcaaca aacgattgcc gttggttaaa tagttttccg cggttgtacc
     2281 aataggattt tccagcagcg ccacgcaatg cagaccgagt ttcgcagcga ctgcggcagt
     2341 ctggcgcaca tggttagact ggatcgcccc ggcagtaatc agcgtatcgg caccttcacg
     2401 cagagcatct gccgcgagaa attccagctt acgtaattta ttgccgccca ttgccatggg
     2461 agtgacgtca tcccgtttga tgaaaatttc ccgtccaaga taatcagaaa agcgcggcag
     2521 atattcgagc ggcgttggcg cgccgataaa ctccagccgt ggaaaacggg ttaaattatg
     2581 cagtggcata acagcctccg atgtgtgttg ttgtgatttt cttattatgc acgctgaaaa
     2641 cgcgtaaata aaaaaggcgc tagtgaaagc gccctttttt gtcattatgc tgattatttg
     2701 gtcacatcag caccaaacca tttttcggaa agggcttgca aagtgccatc tttttgcatt
     2761 tccgcaattg catcattcac cgctttcagc aggtcttcat ttcctttacg cagcgccacg
     2821 ccagactcct gacgggagaa tgcttcaccg gttactgcca gcgtatcgtt ggttttcttc
     2881 accagatcca gcgccgccag acgatcaacg aggatcgcat cgatacggcc tacgcgcaga
     2941 tcctgatatt tggtcgggtc atcatcatag gtacgcacat ccacgccctg aacattctga
     3001 cgcagccact cttcatagtt ggtgcccaga ccgacaccca cttttttgcc tttcagatca
     3061 tcagccgttt taatggtgcc ttcgttacct tttttcacca gcgcctgaat accggaaatg
     3121 gtgtacgggg tagagaaatc gtattttttc ttgcgctcgt cagaaatggt gacctggtta
     3181 atcaccacat caatgcgttt agagtccagc gacgccagca taccgtccca tttagtcggt
     3241 tttagtgacg cctcaacgcc aagatgtttt gccagctgtt gggcaaattc cacttcaaaa
     3301 ccggttaatt tgccgtcatc cccctgaaaa ctgaacggcg gataagttcc ttccagccct
     3361 accagcagcg tgccgcgctc tttgacttta ttaagcagac cttcatctgc aaaactttta
     3421 acgctcatac ccgcaaccag cgccacggcc atcacaccca tcaatgcctg acgtcccaga
     3481 tgtgctaatt tcatatttac cccgaatgtt gttatgtctg tttgcagtgt agagccattg
     3541 tttgtaaaca caaaaacaac tccgctacat cttattctta tttaatatat atcagaagca
     3601 ggttggctgg aggatttctg cacaagtccg gtacacgttt gacgctgata gtgttgatac
     3661 ttccgtaacg cgatgcggta attgttggtg ctggtggagg gcagccacgg ggcgaggatt
     3721 tcatcaagga aaccgtcctg cagctggtcg agggaaatat tttgctcgtg tagatgattc
     3781 cccaaacggc gcagacgaac gacatattca cgcaccgtac cagggctcat ttcagtttgc
     3841 tcaaaaagaa attgcttaaa gccgataata tcgaaaaaat cactctgcgt tttgcaatgt
     3901 aaatcaccgc aaaatcggca caatgccgcc catgattgtt gttccacttg ccagccattt
     3961 tcatcaagca gcgtgtccag gcgggaaatc tcgattttat tcactatttt gccgtcgcga
     4021 actaaggtaa tgcgatcgag taatttacgg caatgcgcgc aatgggtctg gctgtgttta
     4081 aagtctttaa gatagcggct taatggccgt cttttcaggt gctgcaccat cattaagaac
     4141 tcctggtagt caaaattaaa gtgcggcatt tactgacgtt ataacttacc cagtttagtg
     4201 cgtaaccgtt taatagcctg gctgtgtaac tgactgaccc gcgattcccc gacctccagc
     4261 accgcgccaa tctctttgag attcagctct tcctgataat agagggttaa taccagtttt
     4321 tcgcgctccg gcaacgtttc gatagcttcc atcacccgct ggcgcagatt actgtccagc
     4381 agttgttgta gcgggttttc tcgctgatga tcatcagtaa ccagttcaat gctatcgccg
     4441 tgctcttcgc gccactcatc gtaggagaag agctggctgt tattggtgtc gagcaacatt
     4501 tggcgatagt cggcaatatc gatccctaaa cgttccgcta cctcagtttc cgtggcgttg
     4561 cggccaagtt cctgctccag ttgccctatt gcctgtgcca cttcacgcgc gttgcgtcgc
     4621 acgctgcgcg gcacccagtc acggctgcga agttcatcca gcatagcgcc acggatacgc
     4681 tgcactgcgt aagttgtaaa tgccgttcct tgtagggcgt catagcgttc gacggcatta
     4741 agtaacccaa tgccgcccgc ctgtagcaga tcgtcaagtt ccacgctcgc gggcagtcga
     4801 acctgcaggc gcaatgcttc gtgacgcacc agcgggacat aacgctgcca cagcgagtgt
     4861 ttatccatta caccttcagc ggtatagagt gaattcacga taaacagccc tgcgttatat
     4921 gagttatcgg catgattatc cgtttctgca gggtttttaa tcggacgatt agtgggtgaa
     4981 atgaggggtt atttgggggt taccggtaaa ttgcgggcag aaaaaacccc gccggtggcg
     5041 gggtttgaga ggcattcaga cgattaaccc tgcagcagag acagaacctg ctgcggtacc
     5101 tggttggctt ttgccagcac ggagttaccg gcctgctgga taatctgcgc tttcgacatg
     5161 ttggacactt cggtcgcata gtcagcgtcc tgaatacggg actgcgcttc agacaggttg
     5221 gtagtggtgt tgttcaggtt ggtgactgcg gaatccaaac ggttttgcac cgcacccagg
     5281 gaggagcgga atttgtcgat ggagctgatg gcttcgtcca gagctttcag gggatcggct
     5341 gtcgtagctg cttttgtttc agcatctgtc gtcaatttac catcagcatc tttataaact
     5401 gccttaccgc tgccgttagt cactgaacca tcatcctgta ggtaaaaatc tacagtatca
     5461 gatgtggtca acgccccgtt gttatcaaca taaaccgaat caacaccttt tgttacagcg
     5521 ccagttgtgc cgttgacgct ataacctgca gtagtgaaat caacattgtt tcccgaggtt
     5581 gatacagctt tatcgaaagc tgcctgatca acttttgcac ctgttactga ataagtaata
     5641 gtgtccggcg tagtactatt accggtaaag gagatagagg taccgccaat gtcaatggtt
     5701 gtcgctttgg tattattggc tgcaagagtg gatagactag cctcagtcaa agaagcacca
     5761 gagccgtttt tcgttaagcg cccagttgta tcaatgtaga gtttatctcc attgcttgcc
     5821 gtaattttac catcactgct gatctgaaca tcctgtgcag aaccatcgat agtaatagtg
     5881 cccttagcgg tatcaccaac gcccggggtc aagtatttct gaacatcggc agctgaagcc
     5941 gttgtggtat caaaagagta tgacttactt gcactgtcat acttgtaatt cgttgcacta
     6001 gccgccgttc cgaagccgtt attaatggtt gcctgaacag tatcaccatc actaactcca
     6061 gccaacaaat cagacgcttt agcagcatcg taacccgcac tcacagtata tttgttgccg
     6121 accactgttg cattagcagc taccaagtca gctttagatg cagcagtatt agcgacagcc
     6181 ccgccaccat tcacattaaa cccattcagc cccagcgtat cagagtcaat tttcttcaga
     6241 tcaatagtga tagtctggcc gtcattcgca ccaacctgaa ttttcatcga accgtctttt
     6301 gccagtacgt tcacgccgtt gaactgggtc tgaccggata cgcggtcaat ttcgtcgaga
     6361 cgggatttga tttcgtcctg aatggagtcc agatccgaat cagagttagt cccggtagaa
     6421 gcctgaaccg tcagttcacg aatacgctgt aagttgttgt taatttcgga cagcgcgcct
     6481 tcggtggtct gcgcaacaga aataccgtcg ttggcgttac gtgcagcctg agtcaggcct
     6541 ttaatgttag aagtaaaacg gttagcaatc gcctgacccg cggcgtcatc cttcgcgctg
     6601 ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga ctggttcttg
     6661 ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg tgccatgatt
     6721 cgttatccta tattgcaagt cgttgattac gttttgggtt tccacccgtc ggctcaatcg
     6781 ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc acaaacagcc
     6841 attttttgtt agtcgacgaa atactctttt ctctgcccct tattcccgct attaaaaaaa
     6901 acaattaaac gtaaactttg cgcaattcag gccgataacc ccggtattcg ttttacgtgt
     6961 cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg gtcaggtctg
     7021 gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct aacccctatt
     7081 tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct gaaaagcgcg
     7141 ctgacgactt tccagaccgc caatactgca ttgtctaaag ccgatctttt ttccgccacc
     7201 agcaccacca gcagcaccac cgcgttcagt gccaccaccg cgggtaacgc catcgccggg
     7261 aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgaccac gcgcaccacc
     7321 agagatgata cgaaaacggc gatcgccacc agcgacagta aactcaccat tcaacaaggc
     7381 ggcgacaaag atccgattac cattgatatc agcgcggcta actcgtcttt aagcgggatc
     7441 cgtgatgcca tcaacaacgc aaaagcaggt gttagcgcga gcatcattaa cgtgggtaac
     7501 ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgacaatgc gatgacgctc
     7561 tcggttagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag tgccagcagc
     7621 aacggcatgg aggtctcggt cgccgcccag aatgcgcagc tgacggtcaa taacgtcgcc
     7681 attgagaaca gcagcaacac catcagcgac gcgctggaaa acatcaccct gaacctgaac
     7741 gatgtcacca cgggcaacca gacgctaacc atcactcagg acacctccaa agcgcaaacg
     7801 gcgattaaag actgggtgaa tgcctataac tcgctaatag ataccttcag cagcctgacc
     7861 aaatacaccg ccgtagatgc gggagctgat agccagaatt ctagcaatgg cgcactactc
     7921 ggcgactcca cgctgcggac gattcagacg cagttgaaat cgatgctgag taataccgtc
     7981 agttcttcca actataaaac gctggcgcag attggtatca cgaccgatcc cagcgatggc
     8041 aaactggaac tggatgccga caaactcacc gctgcattga aaaaagatgc cagcggcgta
     8101 ggtgcattga ttgttggcga tggtaaaaaa accggcatca cgaccaccat cggcagcaac
     8161 ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg cgttagtaag
     8221 accctgaata agttaactaa agactacaac gccgccagcg atcgcattga tgcgcaggtc
     8281 gcgcgctata aagaacaatt tacccaactg gacgttttaa tgacctcgtt aaacagcacc
     8341 agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc aatattcatc
     8401 gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa
     8461 agcgccgtaa tgagcgccag ccaacagcag ttggtcacca tgctatttga tggagtgctg
     8521 agcgcactgg ttagagcgcg actgtttatg caggacaaca atcagcaagg caaaggtgtc
     8581 tctttgtcaa aagcgatcaa catcattgag aacggactgc gggtgagtct tgatgaaaag
     8641 agcaaagacg aattaaccca aaacttaatt gctctttata gctatatggt caggcgtttg
     8701 ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aggtggaagc attaatgcgt
     8761 aatattgccg atgcctggaa agagtcttta ctctcccctt ctttgattca ggacccagtc
     8821 tgatgaacaa tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc
     8881 tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgccagc gaaatggcgt
     8941 atgtgaatgc ggtgcaggag atcgcacacc tgactgaaga ggttgccccg tccactacga
     9001 tgcaggagca gctccgcccg atgctgcacc tgattctcga caacgaaagc aaggtaaagc
     9061 agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
     9121 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caggataacc
     9181 tcttttgaat cttaatgagc cgatggctcg cgaataatcc gattacggct acgcttctaa
     9241 tattcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
     9301 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgcag acggttttaa
     9361 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcggcta
     9421 ttcggtcggc tacgactcct atgatttatt tgatctaggt gagtttgatc agaaaggcag
     9481 catccctact aaatatggtg ataaagcaca actgctggcc gccattgacg ctctgaaacg
     9541 taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
     9601 agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt acgcaaattg atgaagaaat
     9661 cattgagtgc gaaggctgga cgcgttacac cttccccgcc cgtgccgggc aatactcgca
     9721 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaatc ctgatgaaga
     9781 tggcatattt aaaattgtta acgactacac cggcgaaggc tggaacgatc aggttgatga
     9841 tgaactcggt aatttcgatt atctgatggg tgagaatatc gattttcgca atcatgccgt
     9901 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggctt
     9961 tcgtcttgat gcggtcaaac atattccggc atggttttat aaagagtgga tcgaacacgt
    10021 acaggaagtt gcgccaaagc cgctgtttat tgtagctgaa tactggtcgc atgaagtcga
    10081 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgtttg atgcgccgct
    10141 gcagatgaaa ttccatgaag catcgcgcat gggtcgcgac tacgacatga cgcagatctt
    10201 cactgggacg ctggtggaag ccgatccttt tcacgccgta accctcgttg ccaaccacga
    10261 cacccaaccg ttgcaagccc tcgaagcacc ggtcgaaccg tggtttaaac cgctggcgta
    10321 tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg acctctacgg
    10381 tgcacattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    10441 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt
    10501 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    10561 ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa accattcatc tgggagagaa
    10621 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg tagtgacaga
    10681 cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gttagcgtgt gggttatcga
    10741 agaggtgatt taaatccatc cccggcggca agccggggag atttcattac ggcagtggcg
    10801 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    10861 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    10921 gttttacggt aacgttagtg agatcatcag acatcaatgt caacttaccc ggcacggcaa
    10981 tcacccgctg ccactgacgg caatccaggg tatcgccctc tttagtgaca atgaggctgc
    11041 ctatcgcttc cggactgacc agcgcacgtt gcggcccttt ggtctgccag taacccgtca
    11101 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    11161 ccagcaacat gagcgcacct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    11221 aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggct cagagagaaa
    11281 tttgatctaa ccattatttt tgtgaattat taccccgaaa ataaatttgc atggtgtatg
    11341 attcgcgact cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    11401 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    11461 tattctctcc acttactatt ttggcatcac tggcacattt tgggcagtca cgggtgaatt
    11521 tacccgttgg ggcggtcagc tcctgcagtt gttcggcgtc catgctgaag agtggggtta
    11581 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggaa tgatgatcct
    11641 cggtatgttt ggcggctgtt ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat
    11701 gccgcgcagc cgtatccgca ttatgcaggc catcattggc ggtattatcg ccggttttgg
    11761 cgcacgtctg gcaatgggct gtaacctggc ggcgttcttt accggaattc cacagttctc
    11821 gctgcacgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcgcgctt
    11881 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ctgcctcacc
    11941 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    12001 cggcatgctg ggctgggcgc tgctgacggc gatgaaccag cccaaactgg ggctggcaat
    12061 gctgtttggc gtcggctttg gtttactgat tgaacgtgcg caaatctgct ttacttcggc
    12121 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    12181 ggcggtaagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat
    12241 gtgggcagga ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc
    12301 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    12361 ggtcggtctg ggcaacgtga tcggctcaac gattttggcg tactactggg atgatttcgc
    12421 tccggcgctg gccaccgact gggacaaaat caacctcctg aaaacctttg gtccgatggg
    12481 tggcctgctg gtgacatatt tgctgttgtt tgcagctcta atgttgatta tcggctggga
    12541 aaaacgcttc ttccgccgtg cggcaccgca gattgctaag gagatcgcat gaaaaatatc
    12601 gttcctgatt accgtcttga tatggtgggt gaaccctgcc cttatccggc tgtcgcaacc
    12661 cttgaggcga tgccgcagtt aaaaaaaggg gagatcctgg aagtggtgag cgactgtccg
    12721 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt gctggatatt
    12781 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    12841 gcccacagcg ggaacatcgt tacctctcta tcacatagat tttacccgcc ccctctccta
    12901 ttcttttcag tgtgctctca ggaggtttct atgtgtggac gctttgccca atcccaaacg
    12961 cgtgaagatt acctcgcgtt tctcgcggaa gatattgaac gcgatattcc ctacgatccc
    13021 gaacctattg gcagatacaa cgtcgcgccg ggaaccaaag tccttctgct cagtgaacgt
    13081 gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg gtgggataaa
    13141 ccgccgctga ttaacgcccg cgtagaaact gccgcaacca gtcgtatgtt taaaccgctc
    13201 tggcaacatg gtcgggcaat ctgttttgcc gatggctggt ttgagtggaa aaaagaaggc
    13261 gacaaaaaac agccttattt tatctatcgc gctgatggac aacctgtttt tatagccgcg
    13321 ataggtagca caccgtttga gcgtggtgac gaagccgaag gatttttaat tgtcactgca
    13381 gcagcagacc agggtctggt agatattcat gaccgccgcc cacgagtact gtcgccagaa
    13441 gctgcacggg aatggatgcg gcaagaggtt ggcgataaag aagcctcaga aatcgcggcc
    13501 agtggctgtg ttccagcaaa ccagttcacc tggcacccgg tatcgtgcgc ggtgggtaat
    13561 gttaaaaacc agggcgcgga gttaattcaa cctgtttgat aagcctggag attatctaca
    13621 cctggatgga catcattgta ctatgccccg tacatgcccc atcacatcct caacatcaaa
    13681 gtattagagc cttgcaaacc attatgcgtc tcagtttgtc tcatctatct tac
//
LOCUS       DADSZD010000116        13674 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.117, whole genome shotgun sequence.
ACCESSION   DADSZD010000116 DADSZD010000000
VERSION     DADSZD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13674
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.117"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            47..1501
                     /locus_tag="J1Z28_003154"
     CDS             47..1501
                     /locus_tag="J1Z28_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HBA7717966.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPGG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            1561..2922
                     /gene="allB"
                     /locus_tag="J1Z28_003155"
                     /pseudo
     CDS             1561..2922
                     /gene="allB"
                     /locus_tag="J1Z28_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2978..4279
                     /locus_tag="J1Z28_003156"
     CDS             2978..4279
                     /locus_tag="J1Z28_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBA7717967.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            4301..5446
                     /gene="glxK"
                     /locus_tag="J1Z28_003157"
     CDS             4301..5446
                     /gene="glxK"
                     /locus_tag="J1Z28_003157"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HBA7717968.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVKAFYGITGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQA
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(5575..6360)
                     /gene="allE"
                     /locus_tag="J1Z28_003158"
     CDS             complement(5575..6360)
                     /gene="allE"
                     /locus_tag="J1Z28_003158"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBA7717969.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFV
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(6371..7606)
                     /gene="allC"
                     /locus_tag="J1Z28_003159"
     CDS             complement(6371..7606)
                     /gene="allC"
                     /locus_tag="J1Z28_003159"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBA7717970.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(7628..8677)
                     /gene="allD"
                     /locus_tag="J1Z28_003160"
     CDS             complement(7628..8677)
                     /gene="allD"
                     /locus_tag="J1Z28_003160"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HBA7717971.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            8994..10661
                     /locus_tag="J1Z28_003161"
     CDS             8994..10661
                     /locus_tag="J1Z28_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBA7717972.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSASQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            10671..11930
                     /locus_tag="J1Z28_003162"
     CDS             10671..11930
                     /locus_tag="J1Z28_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBA7717973.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQYIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDVGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            11941..12756
                     /locus_tag="J1Z28_003163"
     CDS             11941..12756
                     /locus_tag="J1Z28_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBA7717974.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGCGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFSVKQPQRYCLLRITPP
                     AHPQPLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFAFSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            12753..13646
                     /locus_tag="J1Z28_003164"
     CDS             12753..13646
                     /locus_tag="J1Z28_003164"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7717975.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPHVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEATYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         3070 a         3339 c         3807 g         3458 t
ORIGIN      
        1 ctagattaaa acaacgttat tcgatacgtg aaattaagag ggatttatgg aacatcagag
       61 aaaactattc cagcaacgcg gctatagcga agatctactg ccgaaaacgc aaagtcagcg
      121 gacctggaaa acatttaact attttacctt atggatgggt tcggttcata acgttcccaa
      181 ttatgtgatg gttggcggct tttttattct cggcttgtct acctttagta ttatgctggc
      241 aattatcctc agtgcctttt tcattgccgc ggtaatggta ttaaacggtg ctgcgggcag
      301 taaatacggc gtaccgtttg ccatgatcct gcgtgcttct tacggcgtac gtggtgcact
      361 gtttcccgga ttattaagag gcggtattgc tgccattatg tggtttggcc tgcaatgtta
      421 cgcggggtca ctggcctgct tgattctgat tggcaaaatc tggccgggat ttttgactct
      481 cggtggcgat ttcacgctgt taggcctttc tctaccggga ttaattactt ttttactctt
      541 ctggctggtt aacgttggta ttggttttgg cggcggaaaa gttttaaata aattcactgc
      601 cattcttaac ccgtgcatct atatcgtttt cggcggtatg gcgatttggg cgatttcgct
      661 ggtcgggatc ggtccaatct ttgactacat tccgggcggt attcagaaag cagaaaacag
      721 tggcttcctg tttctggtgg tgataaacgc ggtggttgcg gtctgggcgg caccggcggt
      781 gagcgcatcc gactttacgc aaaacgccca ctcgtttcgt gagcaggcgc tggggcaaac
      841 gctgggttta gttgtggcct atattctgtt tgcggtcgcc ggggtatgta ttattgctgg
      901 agccagtatt cattacggcg ctgacacctg gaacgtgctg gatattgttc agcgttggga
      961 cagcctgttc gcctcgttct ttgcggtact ggttattctg atgacgacta tctctactaa
     1021 cgccaccggt aatattattc cggctggtta tcagattgcc gccattgcac cgacgaaact
     1081 gacctataaa aacggcgtac tgattgccag tattatcagc ctgctgatct gcccgtggaa
     1141 attaatggaa aatcaggaca gtatttatct gttcctcgat attatcggcg gaatgcttgg
     1201 tccggtaatt ggtgtgatga tggcgcatta ttttgtggtg atgcgtgggc aaattaatct
     1261 tgatgaactg tacaccgcac ctggcgatta taaatattac gataacggtt ttaacctcac
     1321 cgcgttttca gtaactctgg tggccgttat tttatctctt ggcggtaagt ttattccctt
     1381 tatggaaccg ttatcacgtg tttcatggtt tgtcggcgtc atcgtcgcct ttgcggccta
     1441 cgccttatta aagaaacgta caacagcaga aaaaacagga gaacaaaaaa ccataggtta
     1501 attaatcacg atattgaaca ttgagttaaa aaccaatctg tattttacaa ggagtttgtt
     1561 atgtcttttg atttaatcat taaaaacggc accgttattt tagaaaacga agctcgcgtt
     1621 gtagatatcg ccgttaaaga cggaaaaatt gctgctatcg gccaggatct gggcgatgca
     1681 aaagacgtta tggatgcgtc tggtctggtg gtttcgccgg gcatggttga tgcgcacacc
     1741 catatttctg aaccgggtcg cagccactgg gaaggttatg aaaccggtac tcgcgcagca
     1801 gcaaaaggtg gtatcaccac catgatcgaa atgccgctca accagctgcc tgcaacggtt
     1861 gaccgcgcct caattgaact gaagttcgat gccgctaaag gaaagctgac tatcgatgcg
     1921 gcacaactcg gtggcctggt gtcttacaac attgaccgtc tgcatgagtt ggatgaagtg
     1981 ggtgttgtcg gtttcaaatg cttcgttgcg acctgtggcg atcgcggtat cgacaacgac
     2041 ttccgtgacg tcaatgactg gcaattcttc aaaggcgcgc agaagctggg tgaactaggc
     2101 cagccggtgc tggtgcactg cgaaaacgcg ctgatctgtg acgcactggg cgaagaagcg
     2161 aagcgtgaag gtcgcgtaac tgcccatgac tatgtggctt cgcgtccggt atttaccgaa
     2221 gtggaagcga ttcgccgcgt actgtacctg gcgaaagtcg ctggttgccg tctgcacgtt
     2281 tgccacgtca gcagcccgga aggtgttgag gaagtgactc gtgcacgtca ggaaggtcag
     2341 gatgttactt gtgaatcctg accgcattac tttgtgctgg ataccgatca gttcgaagaa
     2401 atcggtactc tggcgaagtg ttcaccgccg atccgcgatc tggaaaacca gaaaggcatg
     2461 tgggaaaaac tgtttaacgg tgaaatagac tgcctggttt ccgaccactc tccatgcccg
     2521 ccggaaatga aagccggtaa catcatgaaa gcgtggggcg gtatcgctgg tctgcaaagc
     2581 tgcatggacg tgatgttcga tgaagcggta cagaaacgcg gaatgtctct gccaatgttc
     2641 ggcaaattaa tggcgactaa cgcagcagat attttcggtc tgcagcaaaa aggccgtatc
     2701 gccccaggaa aagatgccga cttcgtcttc attcagccga atagcagcta tgttcttacc
     2761 aatgacgatc tggaatatcg ccacaaagtc agcccgtatg ttggccgtac tattggcgcg
     2821 cgtatcacga aaaccatctt acgtggtgat gtgatttacg atatcgaaca gggcttccct
     2881 gttgcgccga aaggtcaatt tatccttaaa catcagcagt aatctggccc tgcaatgccc
     2941 gtccttgtgg cgggcattct ccggttaagg tgtgtttatg ttcaattttg cagtgggccg
     3001 cgaaagcctg ttatcaggat ttcagtggtt tttctttatt ttttgcaaca cggttgtggt
     3061 tcctcctacg ctactttctg cttttcagtt gccgcaaagt agcctgctta cgctcacgca
     3121 atatgctttt cttgctaccg cactggcctg cttcgctcag gcgttctgcg gtcatcgtcg
     3181 cgctattatg gaggggccgg gcggcctgtg gtggggaacc atcctgacta tcacccttgg
     3241 cgaagcatcg cgcgggacac cgatcaacga tatcgctacc agtctggcgg tggggattgc
     3301 gctctccggc gtgctgacga tgttgattgg ttttagcgga ttaggccatc gcctggcacg
     3361 gttatttacg ccgtcggtga tggtcttgtt tatgttgatg ctgggtgcgc aactgaccac
     3421 tatctttttc aaaggtatgc tcgggctgcc gtttggcata gccgacccga attttaaaat
     3481 tcaattaccg ccgttcgcgc tctcggtggc ggtgatgtgc ctggtactgg cgatgattat
     3541 tttcctgcca caacgttttg cccgttacgg cctgctggtc ggtacgataa ccggctggtt
     3601 gttgtggtac ttttgctttc cttcttcgca ttcgctctcc ggtgagttgc actggcagtg
     3661 gttcccgctc ggcagtggcg gtgctttgtc gccggggatt attctgacgg cggtgattac
     3721 aggtctggta aatatcagca atacctacgg tgcgattcgg ggcacggatg ttttttatcc
     3781 gcagcagggc gcggggaata cgcgttatcg tcgcagcttt gtggcgaccg gatttatgac
     3841 actgataacc gtgccgctgg cggtaattcc attttcaccg tttgtttcat ccattggttt
     3901 attaacccag actggcgatt acacgcggcg ttcgtttatt tatggcagcg ttatttgcct
     3961 gctggtggcg ctggttccgg cactcacgcg actgttttgc agtatccctt tacccgtgag
     4021 tagtgcggtc atgctggttt cttatctgcc tttactcttt tccgcgctgg tgtttagcca
     4081 gcaaataacg tttaccgctc gcaatattta tcgactcgca ttgccgttat ttgtcggcat
     4141 atttttaatg gcattaccgc ctgtgtatct gcaagacctt ccattaacgc ttcgtcctct
     4201 gctcagtaac ggcttactgg tcggaatttt actggctgtt cttatggata accttattcc
     4261 gtgggaacgc atcgaataat ttgttgaaaa aggattgata atgaagattg tcattgcgcc
     4321 agactctttt aaagagagct taagtgcaga aaaatgttgt caggcaatta aagccgggtt
     4381 ttcgaccctc tttcccgatg cgcactatat ctgtttgccg atagcggatg gcggcgaagg
     4441 gacggtggag gcgatggtcg ccgcgacgga cggtaacatc gtgaagcttg aagtctgcgg
     4501 gccgatgggc gaaaaagtga aagcttttta tggcattacc ggcgacggga aaacggcagt
     4561 gattgagatg gcggcagcaa gtggcctgat gttggtcgcg cctgaaaagc gtaatccgtt
     4621 gctggcctcc agttttggta cgggggagtt aattcgccat gcgctggata acggcattcg
     4681 ccatattatt ctcggcattg gcggcagtgc gacggtcgac ggcggtatgg gcatggcgca
     4741 ggcgctcggt gtgcgtttcc ttgatgccga cggtcaggcg ctggcggcta acggcggcaa
     4801 tttagcgcgc gtggcaagca ttgagatgaa tgaatgcgat ccgcgtctgg cgaactgtca
     4861 tattgaagta gcatgtgacg ttgataaccc gctggtaggg gcacgcggcg cggcggcggt
     4921 gtttggcccg caaaaagggg caacgccgga gatggtcgaa gagctggaac aggggctgca
     4981 aaattacgcc cgtgttttac aacagctaac tgaaatcaac gtctgccaga tggctggtgg
     5041 tggcgcggcg ggcggtatgg gcattgcggc ggcggtattc ctcaatgcgg atattaaacc
     5101 gggtattgag attgtgttga atgcggtcaa tcttgcgcag gcagtgcagg gtgcagcact
     5161 ggtgatcacc ggggaagggc gcatcgactc gcaaacggca ggcggtaaag cgccgctggg
     5221 tgtggcatcg gtggcgaagc agtttaatgt cccggtgatt gggattgctg gcgtattggg
     5281 tgatggcgtg gaagtggtgc accagtacgg cattgatgcg gtattcagca ttttgccacg
     5341 tctggcacct ttagccgaag tgctcgccag cggtgaaacc aatctcttca acagtgcgcg
     5401 aaatattgcc tgcgccatta aaataggtca gggaattaaa aactgactat tacctttaaa
     5461 gcggatgcga tttatatcgc atcttttttt acctgaattg actacaaaaa aagccacggt
     5521 ataaaccgtg gcaaaatcca acatagctaa aaataatcag gcgagtggta tgacttaaat
     5581 ctctacgtcg cggttgcaat ctttcgagta aatatagctg aacgcttcac cacgccctac
     5641 gccataacca gcctgtaaag aataagcgcc cataaagatg taatcgcctt ttttcaccgg
     5701 gatccagtta ttgtcgaggt tataaacccc ctgaccggaa agaatatagg caccgtgttc
     5761 ctgaacatgg gtttcgatat aaccgtggct ggcacctggt gcaaaagaga ggatatgcat
     5821 gttcatatca aaacctaact ctttgggcag aaaatccagc agaataacat cgtccatgcc
     5881 ttcataatga atgcgttcca gttcgctggc attgccagaa accagccacg gcgcatgacc
     5941 ttctaccgga atatagcgac gcttatataa aaagatttgg ctgtcttcgg cctgggcgtt
     6001 aacaaacgtc attaaggagc ctggcgggca ataaagatag ccaccttcgc ttaagacaaa
     6061 tgttttccct tcggctttgg cagtgatatt tccagagatc acatacagga acgtttcaat
     6121 gccttcgcca ccgaagccct gttggttgcc gccgttttga tgcagtgtga ccagataatc
     6181 aacaaaagag gcacccagct ttggcgtgga gaggattgtc gcgtcacaat tttcaaagcc
     6241 cggaataata ttttttacca gaccgtctgg tgttaacagt gcgaaattac cgtgtttaac
     6301 aatcgcacgg ttagccagta aatcttcgcg gtaaccggtg acgttattta aatatcccat
     6361 ttataactcc ttatttctgc caggcaagtt gataaagcat gagtgccaac gttttgaccc
     6421 cttcggcaag gtcggtaata ttggtgcgtt ccgccgggtt atggctgatc ccgttgatgc
     6481 tgggaatgaa aatcatgcag gtcggcacgc gaggcgcgaa aatttgcgcg tcgtgcccgg
     6541 caccactgtg catcacccgg taattcagtt tttcgctttc acacaattct gtcagggtgg
     6601 cgaccagctc cttattcatc ggcacgggtt cttcgtccat ccataaatcg atatcaatac
     6661 caatatccat ttcatcgcaa atcgcccgca tgtcgttttc taactgttgg gtgaaatcgc
     6721 gcagcacggt agcatcggta tgacgacaat caatggtgaa cgtggtttta cccggcacta
     6781 cattcaccgt attcgggcgc ggctctactt tgccaaaggt cagaaccagc gggtcgccca
     6841 tctttttcgc tttttcgaca gactgatggc aaatgcgact gaaagcgtaa accgtatcac
     6901 gacgatagcc catcggcgtg gtgcctgcat ggtttgattc accgttcagc gtcaccgtat
     6961 aacgacgctg cccgacaatt gcattcacca cgccaattga ttgcccatta ctttccagca
     7021 cacagccctg ttcgatatgc agttcgacaa aggctttaat atcctgacgc ggagttagtg
     7081 gggcgttcgg cagagtaaat ccgcaagcct tcatcgcatc gacaaaacta tttcctttgg
     7141 catcacagat attccgcacg tcgtcaggat tcgccagccc aaaaatattt ttactgcccc
     7201 agaaaacata cgggaagcgg ctgccttctt cttctgccat cgccaccact tcgaccgtac
     7261 gtaacggcgc gccgtattgc gttttcagcc agtcaatagc cagccacgcc gccagcgcac
     7321 cgaattgccc gtcgaggtta ccgccgttaa ccacggtatc gatatgcgaa ccgctcagaa
     7381 ccacttcctg tggatattcg gtgccactca ggcgaccgta taaattcccc acttcatcga
     7441 aacgtgtttc cagcccgctt gctgccattc tttttttaaa ctgctgctgg gtttccagcc
     7501 attccggcga ataaagtaaa cgggtcatcc cacccgctgg gtcagcgcca aaagaggaaa
     7561 gccagggcag cgtttcttct atagcttgac ggaaatgtgt aatcataaga aagtcctgtc
     7621 tcaataatta ttgcgcaaag ggatttttcg tttcgtatga cgtgttataa agcgcgtcgg
     7681 aaattaaata ctggtaaata tcatcaacaa tttcgatgcc ttcgacggcg gctttgcgtt
     7741 gtttaatatc ctgatcctgt ccgggataat aaacctgatt aaaaccgggc gcgggggtaa
     7801 tggtatttaa ttcgcgcatg gtctggctaa gatgttgacg gaataattcg ctggaggaga
     7861 aaaagttcgg gttaataact atatgtaatt gccccaaatt acgcccggcg tgtaaatcgt
     7921 catacatcga actaacctgt cgcccgaacg gtaagccgag caagacgcct gagaggacgt
     7981 caatcatcat catcaggcca taccctttcg gcccagcggc ggggagcaga gcatgtaccg
     8041 cgaacggatc ggttgttggt gcaccgtttt tatcgactgc ccaggtatcc gggatagaca
     8101 tattgcgcga gcgggcgtcg agcacttttc cccatgcctg tacggtagtc gccatatcaa
     8161 aggtaaggat ctcgtcgcct tctcccggcg cggcaaaggc cagtgggtta gtgccgtagt
     8221 aaatttccgc gccaccaaac ggtaccacca ttggatcgga ctggcacagc gaaatgccaa
     8281 tcaatccggc gcgggcagcc tgctgcacaa aataagagat tgcgccgctg tgacccatcc
     8341 ggctgatacc gaccaccgca acgccttttt gctgggcggt tttgatggca tgttccatac
     8401 ccattttcgc cgcgacctgt ccggcggcat tgtcggcatg taaaattgcc gagcacggtc
     8461 cggtttcctc aagacgaaac tccggttcgc ggttggtgcc gccttttgaa atgcgttcgg
     8521 cgtagtattc aacgcgcacc gcgccatgag agtggatccc tctggcatcg gcgtaaacca
     8581 atacttcagc cacggttgca gcgtgctcac gttttaaccc agcctggcag agtttattct
     8641 cgattagctg gtggagtgtt tcccgactga ttttcatctg tcttcctttt taacgacggt
     8701 gtgaagcatg actgcaatta acatacaggg aaaatatctg gattatgtga tccagacagg
     8761 caaaaaaata tagttagaat ttatttgata atcttcgtgc ttttaacctg gtttttaaaa
     8821 caaccatgct tattaaaaaa taacgcgtaa tagtctggcg gttatttgaa ttcttttgtt
     8881 aataatccac gtgtgatatt catcacctta tttactcgtt gtcatcgata ccgtaatcgc
     8941 cacattaaca ctgctcgtgc aattgccatg ggtgcaattt ttaaggagtt gttatgatcc
     9001 acgcctttat taaaaaaggg tgttttcagg attcggtcag tttaatgatt atttcacgaa
     9061 aactcagcga atcagaaaat gttgatgatg tttccgtaat gatgggtacg cccgccaata
     9121 aagcgttatt agatactaca ggtttctggc atgacgattt taataacgcc acgccgaacg
     9181 atatttgcgt ggcaattcgt agcgaagcgg cggatgcggg gatcgcgcag gcagttatgc
     9241 agcagcttga agaggcgcta aaacaactgg cgcagggttc aggcagcagc caggcgttga
     9301 cgcaggtgcg tcgctgggac agtgccagtc agaaattacc cgatgccaat ctggcgctga
     9361 tttcagtggc tggtgagtat gcggcggagc tggcgaacca ggcgctggat cgcaacctca
     9421 acgtgatgat gttctccgat aacgtcacgc tggaagatga aatccaactt aaaacccgcg
     9481 cgcgggaaaa aggcttgctg gtgatggggc cagactgcgg tacgtcgatg attgccgcca
     9541 cgccgctggc ttttgctaac gtgatgccgg aaggcaacat tggcgtcata ggcgcttccg
     9601 gtaccgggat tcaggagctg tgctcacaga ttgcgctggc aggggaggga attactcacg
     9661 cgattggtct tggcgggcgc gatctcagcc gtgaagtggg cggcatcagt gcgctaacgg
     9721 cgctggaaat gctcagtgca gacgagaaaa gcgaagtgct ggcatttgtt tcaaaaccac
     9781 ctgccgaaac tgtgcgtctg aaaattgtta atgccatgaa agcaactggc aaaccgacgg
     9841 tggcgctgtt tttaggttat accccggcgg tggcccgcga cgagaatgtc tggtttgcct
     9901 cctcgctgga tgaggccgct cgtctggctt gtctgctttc acgcgtcact gcgcgacgta
     9961 acgcgatagc gcctgtcagc agcggattta tttgcggttt gtataccggc ggtacgctgg
    10021 ctgctgaagc ggcgggatta cttgccggac accttggcgt ggaagccgac gatacccatc
    10081 aacatggcat gatgctggac gccgacggac atcagatcct cgacctgggc gatgattttt
    10141 acaccgtcgg gcgtccccat ccgatgatcg acccgacctt acgcaacctg ttaattgccg
    10201 atctcggcgc taaaccgcaa gtgcgcgtgt tgctgcttga tgtcgtgatt ggcttcggtg
    10261 cgaccgccga tcctgccgcc tcgctggtga gcgcctggca aaaagcctgt gccgcgcgtt
    10321 cagacaatca accactgtat gccattgcca cggtgacagg cactgaacgt gacccgcaat
    10381 gccgctcgca gcaaatcgcc acgctggaag atgcggggat cgcggtagtg agttcgctac
    10441 cggaagccac cttgctggct gcggcgttaa ttcatccgct ctcgcctgcc acacagcaac
    10501 acacaccgtc attactggaa aacgtcgccg tgattaacat cggattacgc agctttgcgc
    10561 tggagctaca aagcgccagc aaaccggttg tgcattacca atggtcgcca gtcgccggcg
    10621 gcaataaaaa actggctcgt ttattagaac gtttgcaata aggggttccc atgtttacat
    10681 cagtggcgca agccaatgct gcggttatcg aacaaattcg tcgcgctcgt ccacactggc
    10741 tggatgtgca accggcttct tcacttatca gcgaactaaa caagggcaaa acactgcttc
    10801 acgccgggcc gccaatgcgc tggcaggaga tgaccggacc catgaaaggg gcgtgcgtgg
    10861 gcgcatgtct gttcgaaggc tgggcgaaag atgaagcgca ggcgctggca atactggagc
    10921 agggggaagt gaacttcatt ccttgtcacc atgtgaatgc cgtcgggcca atgggtggca
    10981 ttacttctgc cagtatgccg atgctggtgg tcgagaacgt gaccgacggc aaccgggcgt
    11041 actgcaacct caacgaaggt atcggcaaag tgatgcgttt tggcgcttac ggcgaagatg
    11101 tcctgactcg ccatcgctgg atgcgcgatg tgttaatgcc agtattaagc gcggcgctgg
    11161 ggcgcatgga gcgcggtatc gatctcaccg cgatgatggc gcagggcatt accatgggcg
    11221 atgagttcca tcaacgcaat attgcttcct ctgcactgtt aatgcgtgcg ctggccccac
    11281 aaattgctcg cctcgatcat gataaacagt acattgcgga agtgatggat ttcctcagcg
    11341 tgaccgatca gttcttcctc aacctcgcga tggcctactg caaggcggcg atggatgtag
    11401 gcgcgatgat ccgcgcaggc agcatcgtta cggctatgac ccgcaacggc aatatgttcg
    11461 ggattcgggt aagcgggctg ggtgaacgct ggtttactgc gcctgtaaac actccgcaag
    11521 gtctgttttt caccggcttc tcgcaggagc aggcgaaccc ggatatgggc gatagcgcga
    11581 ttaccgaaac ctttggtatc ggaggtgcgg caatgatcgc cgcacctggc gtaacacgct
    11641 ttgtcggtgc gggtggcatg gaagcggcca gagcggtatc tgaagagatg gcggaaattt
    11701 accttgaacg caatatgcaa ttgcagatcc ccggctggga tttccagggg gcgtgcctgg
    11761 ggctggacat tcgtcgcgtg gtagaaaccg gtattacgcc actcatcaat accggtatcg
    11821 cccataaaga ggcggggatc gggcagattg gcgcaggcac cgtgcgggca ccgctggcgt
    11881 gctttgaaca ggcgctggaa gcactggctg aaagcatggg gattggttga ggaacgcaca
    11941 atgacgatca tccatcctct acttgccagc cgtagcgcac cgaattatcg ccagtcctgg
    12001 cggttagcgg gagtgtggcg gcgggcgatt aacctgatga cggaaagcgg cgaactgtta
    12061 acgctgcatc gccaggggag tggttgcggc cccggcggat gggtgcttcg ccgcgcgcaa
    12121 ttcgatgcgt tatgcggtgg attatgcggc aatgaacgac cacaggttgt ggcacaaggg
    12181 attcgcctcg ggcgtttctc ggttaaacag ccacagcgtt attgtttgct acgtattacg
    12241 ccgcctgcgc atcctcaacc gcttgccgtt gcatggatgc aacgcgcgga ggaaaccggg
    12301 cttttcgggc cactggcatt ggcggcaagc gatccgctgc ctgctgaatt acgccagttt
    12361 cgtcactgtt ttcaggccgc gctcaatggc gttaagaccg actggcggca ctggctgggt
    12421 aaaggccccg gattaacgcc aagtcatgat gacacgctga gcggaatgct gctggcggcc
    12481 tggtattatg gggctttaga tgcgcgctcc ggtcgcccgt tttttgcctt ttccgacaat
    12541 cttcaactcg ttaccactgc ggtgagcgtc agttatttac gttatgccgc gcaaggatat
    12601 ttcgcctcgc cactcctgca ctttgttcat gctctgagtt gtccgaaacg taccgcagcg
    12661 gcgatcgatt cgctgctggc gctggggcat acgtcagggg ccgatacgct gctggggttc
    12721 tggcttggcc aacaattatt acaaggaaaa ccatgaaaac actggttgtg gctcttgggg
    12781 gcaacgcctt actccagcgc ggtgaggcgc tgacggcaga aaatcaatat cgcaatattg
    12841 ccagtgctgt gcccgcgctg gcgcgcctgg cccgttctta tcggctggcg attgttcacg
    12901 gcaacgggcc gcaggtgggg ctgctggcgt tacagaatct ggcgtggaaa gaggtagaac
    12961 cgtatccgct ggatgtactg gtggcggaaa gccaggggat gattggctat atgctggcgc
    13021 agagcttgag cgcacagccg cagatgccgc atgtcacgac ggtgctgacg cgcattgagg
    13081 tttcgcctga tgatccggcg tttttgcagc cagagaagtt tattggtccg gtttatcagc
    13141 cagaagaaca agaggcactg gaagcgactt acggctggca gatgaaacgt gatggtaaat
    13201 atttgcgccg ggtggtggcg tctccgcaac cgcgtaaaat tctcgacagc gaagccatcg
    13261 agttgttgct caaagagggg catgtggtga tttgcagtgg cggcggcggt gtgcctgtga
    13321 cggaagacgg ggcagggagt gaagcggtga ttgataaaga tctcgccgct gcgttgctcg
    13381 ccgagcagat taatgccgat ggactggtga tcctcaccga tgctgatgcg gtatatgaaa
    13441 actggggaac gccgcagcaa cgtgccattc gccatgccac accggatgag ttagcgccat
    13501 ttgccaaagc cgatggttcg atggggccga aggtaacggc ggtgagtggc tatgtcagaa
    13561 gccgtggtaa acccgcgtgg attggggcgt tatcgcggat tgaagagacg ctggcgggcg
    13621 aagcggggac ttgtatttcg ctgtagtgat gggcaccagg caattggcac atct
//
LOCUS       DADSZD010000117        13617 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.118, whole genome shotgun sequence.
ACCESSION   DADSZD010000117 DADSZD010000000
VERSION     DADSZD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.118"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1356
                     /gene="chbC"
                     /locus_tag="J1Z28_003165"
     CDS             <1..1356
                     /gene="chbC"
                     /locus_tag="J1Z28_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBA7717976.1"
                     /translation="SNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMF
                     VLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIG
                     MALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAE
                     MFTFIVHRNWVIKLPDSVPTSVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIM
                     DTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQY
                     GSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALP
                     SGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMP
                     TGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALK
                     F"
     gene            1407..1757
                     /gene="chbA"
                     /locus_tag="J1Z28_003166"
     CDS             1407..1757
                     /gene="chbA"
                     /locus_tag="J1Z28_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBA7717977.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            1768..2607
                     /gene="chbR"
                     /locus_tag="J1Z28_003167"
     CDS             1768..2607
                     /gene="chbR"
                     /locus_tag="J1Z28_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBA7717978.1"
                     /translation="MQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDYY
                     EFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFEQ
                     HYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRHY
                     REEQVIDDIPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTPM
                     QIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLTE
                     FNQ"
     gene            2711..4063
                     /locus_tag="J1Z28_003168"
     CDS             2711..4063
                     /locus_tag="J1Z28_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBA7717979.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLTARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSMNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELNVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCTLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            4076..4834
                     /gene="chbG"
                     /locus_tag="J1Z28_003169"
     CDS             4076..4834
                     /gene="chbG"
                     /locus_tag="J1Z28_003169"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBA7717980.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSA
                     YCFPRLTELDVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(4881..7141)
                     /gene="katE"
                     /locus_tag="J1Z28_003170"
                     /pseudo
     CDS             complement(4881..7141)
                     /gene="katE"
                     /locus_tag="J1Z28_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
     gene            7324..7587
                     /gene="cedA"
                     /locus_tag="J1Z28_003171"
     CDS             7324..7587
                     /gene="cedA"
                     /locus_tag="J1Z28_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBA7717981.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(7634..9025)
                     /locus_tag="J1Z28_003172"
     CDS             complement(7634..9025)
                     /locus_tag="J1Z28_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HBA7717982.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(9158..9748)
                     /locus_tag="J1Z28_003173"
     CDS             complement(9158..9748)
                     /locus_tag="J1Z28_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7717983.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIASAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(9912..10580)
                     /gene="hxpB"
                     /locus_tag="J1Z28_003174"
     CDS             complement(9912..10580)
                     /gene="hxpB"
                     /locus_tag="J1Z28_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBA7717984.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            10727..11263
                     /locus_tag="J1Z28_003175"
     CDS             10727..11263
                     /locus_tag="J1Z28_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7717985.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNVFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PLICIAAGYVFFSLLGFI"
     gene            complement(11304..12164)
                     /locus_tag="J1Z28_003176"
     CDS             complement(11304..12164)
                     /locus_tag="J1Z28_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBA7717986.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(12270..12560)
                     /gene="ghoS"
                     /locus_tag="J1Z28_003177"
     CDS             complement(12270..12560)
                     /gene="ghoS"
                     /locus_tag="J1Z28_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBA7717987.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMMDDDG
                     NIHELGTNTFGLISTQNEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(12661..13590)
                     /gene="pfkB"
                     /locus_tag="J1Z28_003178"
     CDS             complement(12661..13590)
                     /gene="pfkB"
                     /locus_tag="J1Z28_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBA7717988.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGTATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHLEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         3434 a         3207 c         3468 g         3508 t
ORIGIN      
        1 agtaatgtta ttgcatcgct tgaaaaggta ctcctccctt ttgcagttaa aataggaaag
       61 cagccacacg ttaatgcaat caaaaatggc tttattcgct taatgccgtt aacccttgcg
      121 ggggccatgt ttgtattaat taacaacgtc tttctaagct ttggggaggg gtcgtttttt
      181 tattccttag gtattcgcct ggatgcctca accattgaaa cacttaatgg tctgaaaggt
      241 attggcggca acgtatataa cggaacatta gggataatgt ctttaatggc accgttcttt
      301 attggcatgg cgctggcaga agagcgtaaa gtcgatgcgc tggcggccgg gttgttatcc
      361 gttgcagcat ttatgaccgt caccccatat agtgtcggtg aggcctatgc ggttggcgca
      421 aactggttag gtggggcgaa tatcatctcc gggattatta ttggcctggt ggtggcagaa
      481 atgtttacct ttattgttca tcgtaactgg gtcattaagt tacctgatag cgttccgaca
      541 tcggtttcgc gctctttctc agcattaatt cctggtttta ttattctttc cgtgatgggg
      601 attattgcct gggcgttgaa tacctggggc accaacttcc atcagatcat tatggatacc
      661 atctcaaccc cactggcatc gttgggtagc gtggtaggct gggcctatgt gatctttgtt
      721 ccactgctct ggttcttcgg tattcatggc gcgctggcgc tgaccgcact ggacaacggc
      781 attatgacgc cgtgggcgct ggaaaatatc gcgacctatc agcaatatgg ttccgtcgaa
      841 gcggcgctgg cagccggtaa gaccttccat atctgggcca agccgatgct ggactccttt
      901 attttccttg gtggcagtgg tgcgacttta ggcctgatcc tggctatctt tatcgcttct
      961 cgccgtgctg attatcgtca ggtggcaaaa ctggcgctgc cgtccggcat cttccagatt
     1021 aacgaaccga ttctgtttgg tctgccaatt atcatgaacc cggtgatgtt tatcccgttt
     1081 gtactggtac aaccgattct ggcggcaatc accctggcag cgtactacat gggcattatt
     1141 ccaccggtga ccaatattgc accgtggacc atgccaaccg gtctgggagc cttctttaac
     1201 accaacggta gcgtcgccgc attgctggtc gcactcttca atcttggtat cgcaacgtta
     1261 atttatctgc cctttgttgt ggtcgctaac aaagcacaaa acgcgattga taaagaagag
     1321 agcgaagaag atatcgccaa cgccctgaaa ttttaattga tgccggtacg ggtaatcgtg
     1381 ccggtaagaa tagagaggaa cgatgtatga tggatctcga taatattccc gatacgcaaa
     1441 cggaagctga agagctggaa gaagtggtga tggggcttat catcaactcc ggacaagcgc
     1501 gtagcctggc gtatgcggca ctgaaacagg cgaagcaggg cgattttgcc gcagcaaaag
     1561 cgatgatgga tcagtcacgg atggcattga atgaagcgca tctggtacag acgaaactga
     1621 ttgaaggcga tgctggcgaa ggtaagatga aagtgagtct ggtgctagtc cacgctcagg
     1681 atcatttaat gacgtccatg cttgcgcgtg aactgattac tgaattaatt gagcttcatg
     1741 aaaaactgaa ggcataagga gtcgatgatg cagccagtga ttaacgcgcc ggaaattgcc
     1801 actgcccgag aacagcagtt gtttaatggc aaaaacttcc atgtgtttat ctacaataaa
     1861 actgagagta tcagcggact gcatcagcac gactattatg aatttactct ggtattaacc
     1921 gggcgttatt tccaggagat taacggtaag cgcgtgttac tggaacgggg cgattttgtt
     1981 tttattccgt taggttcgca ccatcaaagt ttttatgaat ttggtgccac gcgtatattg
     2041 aacgttggga tcagtaaacg cttttttgag cagcattacc tgccgttgtt gccttattgc
     2101 tttgtagctt cgcaggtata ccggaccaat aacgcttttc tcacctatgt ggaaacagtg
     2161 atttcttcat tgaatttccg cgaaacaggg ctggaagagt ttgttgagat ggttactttt
     2221 tatgtcatta accgtttacg tcattaccgc gaagaacagg tgattgatga tataccgcag
     2281 tggctgaaaa gtacggtaga aaagatgcat gataaagagc agtttagtga atcggcgctg
     2341 gagaatatgg tgacgttgtc agccaaatca caggaatatt tgacgcgagc gactcaacga
     2401 tactatggta aaacgccaat gcagattatt aatgaaatcc gtattaattt tgccaaaaaa
     2461 caactggaaa tgaccaacta ttcagtgacg gatattgcgt ttgaggccgg ttatagtagc
     2521 ccgagcttgt ttattaaaac gtttaagaaa ttaacatcct ttacgcctaa gagctatcgt
     2581 aagaaattga ctgaatttaa tcagtaagtc gttatacctg acaattcaca tatctgtcct
     2641 gtagctggat tatttatgtc cggggggcag atatgccaga tatcagtatt ctgtactgaa
     2701 gggagaaatt atgagccaga aattaaaagt cgtcactatt ggtggcggga gcagctatac
     2761 cccggagtta ctggaaggat ttattaagcg ttatcacgaa ttgcctgtca gcgaattatg
     2821 gctggtggat gtcgaaggtg gtaaagcgaa actggatatt atttttgatc tctgccaacg
     2881 gatgattgat aacgctggcg tcccgatgaa gctttataaa acgctggatc gccgcgaagc
     2941 attgaaagat gctgatttcg ttactaccca actgcgcgtt ggccaattaa cggcacggga
     3001 actggatgaa cgtattccat taagtcatgg ttatcttggt caggaaacta acggcgcggg
     3061 cggtctgttt aaaggtctgc gtaccattcc ggtgattttt gacatcgtaa aagatgtcga
     3121 agaactatgt ccgaatgcat gggtgattaa cttcactaac ccggcgggaa tggtcactga
     3181 agccgtttat cgtcataccg gatttaaacg ctttatcggc gtgtgtaata ttccgatcgg
     3241 catgaagatg tttattcgcg atgttctgat gctgaaagac agcgatgatt tatctatcga
     3301 tctgttcggc ctcaaccata tggtgttcat taaggatgtg ctggtaaatg gcaaatcacg
     3361 ctttgccgaa ttgcttgatg gtgtggcatc cgggcagtta aaagcatctg gcgttaaaaa
     3421 tattttcgat ctgccattta gcgaaggctt aattcgttct atgaatctgt tgccgtgttc
     3481 ttatttgctt tattacttca agcaaaaaga gatgctggct attgaaatgg gcgaatacta
     3541 caaaggcggc gcacgagcgc aggtagtaca gaaagtcgag aaacaacttt ttgagctata
     3601 taaaaacccg gagttgaacg ttaagccgaa agaactggaa cagcgcggtg gggcttatta
     3661 ttctgatgct gcgtgcgaag tgatcaacgc tatctataac gacaagcaag cagaacatta
     3721 cgttaatatc ccgcatcatg ggcatattga taatattccg gcagactggg cggtagaaat
     3781 gacctgtacg ctggggcgcg atggcgcgac gccacatccg cgcattacgc atttcgatga
     3841 taaagtgatg gggctgattc acaccatcaa aggcttcgag attgctgcca gcaacgccgc
     3901 acttagtgga gaatttaacg atgtcttact ggcgctaaat cttagtccgt tggtgcattc
     3961 cgatcgcgat gctgagctgc tggcacgcga gatgattctg gcgcacgaga aatggctgcc
     4021 aaattttgcc gactgcatcg cagagcttaa aaaagcacat taaccgaggc tgattatgga
     4081 acgcttactg attgttaatg ccgatgattt tggcttaagc aaaggtcaga actacggcat
     4141 tatcgaggcc tgtcgcaatg ggattgtcac gtcgacgacg gcgctggtga atgggcaggc
     4201 tattgaccat gcggtgcagt tgagccgtga tgaaccgagt ctggccatag ggatgcactt
     4261 tgtccttact atgggcaagc ctctgacagc tatgccgggg ttaacccgcg atggtgtgct
     4321 gggaaaatgg atctggcagt tggcagaaga agatgcttta ccgctggaag aaattactca
     4381 ggagctcgcc agtcagtatt tgcgtttcat tgagctattt ggacgcaaac ctacgcatct
     4441 tgatagccat catcatgtgc atatgttccc gcagattttc ccgattgtgg caaagtttgc
     4501 ggctgaagag gggattgcgt tgcgcatcga ccgtcaaccg ctatctaacg atggtgattt
     4561 accggctaat ctgcgcagtt cgcagggatt cagtagcgcg ttttatggtg aagagattag
     4621 cgaggcattg ttcctgcaag tgcttgatga ttccagccat cgcggggaac gttcgttgga
     4681 agtcatgtgc catccggcgt ttgtcgataa tacgattcgc cagagtgctt attgcttccc
     4741 tcggttaacg gaactggatg tgctgacttc agcgtcgttg aaatatgcga ttgctgagcg
     4801 tggttatcgg ttgggtagtt atcatgatgt gtaagtaagg tggtaaggtc actccccagg
     4861 gggcgtgacc ttatccatat ttacgccggg attttgtcaa tcttaggaat gcgtgaccac
     4921 acgcggtgtg ctgtcatcag cgttaacagt tcatccataa aactaccatc ggcgctgtca
     4981 gcttccgcaa tcccttcttc accttggtca gcgaccttga ttgttgcttt aaacttgcgc
     5041 gcgtctcctg ccagcgcaat cggtttaagg tgtttgtagg cttccatcag atagtagttg
     5101 gcatcgccgt tgttagcgat atccgcaata ttgccgcaag ggacaatgac tgcatcgacc
     5161 gtcagcgaag gcgcaccggc aaaggtagca gctataggca acaccgtacc gtcatccgca
     5221 gtcacttccc ccattcggga gtagagcagt ttggcatgaa cgcctttggc cttcagtgcc
     5281 ttgagaatgg ccagaaggtc tgccgatctc acttcatcat taagcaaaat tgctaccacg
     5341 cgacctttca cataccgtca ggaatggcgt acagacttaa ggatggatcc tttttcagac
     5401 cgttgacgtc cggaggcggg gtgatattca actggtcgtc agtcagttcg ataccgagat
     5461 ttttcgccac cgcctgggcc agagtgagat caatatgcgc cagctggtca acaacgcgct
     5521 cacgaatata cggacgaact actttgctta actcaaaact gaaaccatcg acaatatggt
     5581 gctgctcgaa tggcgtctga cttagccaga acagacgcgg atgggaataa tattcgccaa
     5641 acgatggact gcgctcgcga actttattcc cttccacgcg ttcctggtat gattcaaaac
     5701 cgccgcgttt cggccccggc ggtgtttcgc gcggccagtt atcgttgatc gagttcggtt
     5761 cgtaattcgc agggttagtg tcgatcccca tacgatgcat gccgtcacgc tggaaattat
     5821 ggtaagggca ggtcgggcgg ttaatcggaa tttcatggaa attcggccca ccaagacgac
     5881 tgatttgtgt atcggtataa gagaacaaac gcccctgcaa taacgggtcg ttggtgaaat
     5941 ccagaccggg gacaatatgc cctggatgga aagccacctg ttcgttttca gcaaagaagt
     6001 tatccggatt acgattgagc accattttgc cgacacgctg aacaggcacc aattcttccg
     6061 ggataagttt tgttggatcg agaagatcga agtcgaactt gaattcgtct tcttcaggaa
     6121 tcaactggaa gcccagttcg tattcaggaa aatcgcctgc ttcaatcgct tcccacaact
     6181 cgcggcggtg gaagtccggg tcacgtccgg ttagtttttg tgcttcatcc caaacgagtg
     6241 aggctttacc tgccagtggt ttccagtgga aacgtacgaa cgttgccttc ccttcggcat
     6301 taatcaggcg gaaggtatga ataccgaagc cttccatagt gcggtaactg cgcgggatac
     6361 cgcgatccga cattgcccac atcacgttgt gcagagtttc aggttgcaga gaaacataat
     6421 cccagaaagt atcgtgggcg ctttgccctt gtggaattgc ccagtgcggt tctggtttta
     6481 ccgcatgaac aaaatcgggg aatttatgcg catcctggat aaagaagatt ggcgtgttat
     6541 tgccaacgag gtcaaaaata ccctcttcag tatagaactt ggtggcaaag ccacggatat
     6601 cacgtacggt atcggcagag ccagcgccgc cctgaacggt agagaaccta acaaatactg
     6661 gggtgatttt gttcgtatct gagaggaaat ccgctttggt gatatcgctt aagcttttat
     6721 atggctggaa ataaccgtga gctgctgatc cgcgtgcatg aacaatacgt tccgggatgc
     6781 gctcatggtc aaagtgagtg attttctcgc gcagaataaa atcttccagc agagttggac
     6841 cacggctacc ggcacgcagt gagttttgat cgtcggcgat gcgcacgccc tgattagtgg
     6901 tcagcgcata attttcactg cctttgcgta cgtcttccag agaattaagt ttttcgttac
     6961 gcgtatcagg ggctttcagg ctccctgggg cggtaggttg tgcaccaggc ggtgttggtt
     7021 cagtcgctgg acgatgagat ccgtcttcag gtgccagtga gtccatcccc ggtttcgctt
     7081 cgctggaatc gtgtagtggt gactggtgct gatgtgggtt cttttcgtta tgttgcgaca
     7141 ttgaactcgt ctccttaaat tattactgaa agggccgcta ttttggtcaa aaaaccctct
     7201 aactatagac caccagccag atcccgcttc ggaaactatt ctggaaacgg tgttatgaaa
     7261 tacacgcaaa gcgggacgta caggggctaa atcggctaaa ctacagtttt tctgattttt
     7321 aatatgcgtt tagtgaagcc agtaatgaag aaaccgctcc gtcagcaaaa ccgccagatt
     7381 attagctatg tcccacgcac ggaacccgcg ccgccagaac atgcgataaa gatggattcg
     7441 tttcgagatg tctggatgct gcgtggcaaa tatgttgcgt ttgtactgat gggggagtca
     7501 tttctgcgct caccggcgtt tactgtgcct gaatccgccc agcgttgggc aaatcagatt
     7561 cgtcaggaaa atgaggtcga agaataaaaa aacacctgtc gggatgacag gcgtttaacg
     7621 taacaaatca ggattaacgg tgtgccagtt cggcgtcgtc ttcgctatcc agaatggctt
     7681 tatcggtttg cttcagccac tggctggtca gcgtgccagc ggtcatcgag ccactaacgt
     7741 ttagcgccgt acgacccatg tcgataagcg gttcaacgga gattaacaat gccaccagcg
     7801 ttaccggcag gcccatcgca ggcagcacaa tcagtgcggc gaaagttgca ccaccaccga
     7861 caccggcaac gcctgcggaa ctaacggtga caataccgac cagcgtcgca atccacatcg
     7921 ggtccagtgg gttaatgccc accgtaggcg caaccatcac cgccagcatt gccggataca
     7981 aaccggcaca accgttctga ccaatggtcg caccgaaaga ggcggcgaaa ctggcgatgg
     8041 attcaggaac acccagacga cgcgtttgtg cttccacatt cagtgggata gacgcagcac
     8101 tggaacggct ggtaaaggca aacgtcagta ctggccatac cttacggaag tacttcagcg
     8161 ggctcacgcc attaatgccc agcagaaggc catgcactgc aaacataatc agcagaccga
     8221 ggtaggacgc aacaacgaaa cttcccagtt tgatgatgtc ttgcaggtta gaacctgcaa
     8281 ccactttggt catcagcgcc agaacaccgt aaggggtcaa ctgcatgacc aggcgaacca
     8341 gtttcatcac ccagctttgc agggtatcga tagcggttaa gacgcgttca cctttcggcg
     8401 catcatcctt cagcagtttc agcgcagcta cgccgaggaa tgcggcaaaa attaccacgc
     8461 tgataattga cgtcggattg gctccggtca gatcggcaaa cgggtttttc gggataaagg
     8521 acaagaccag ctgcggaacg ctcaggtcag agactttacc aacatagtta ctttcgatgg
     8581 cgttcagacg tgcagtttct gcaccaccct gaaccagacc ttcagccgtc aaaccaaaca
     8641 ggttggtgac cagcacaccg accagcgctg caatcagcgt ggtaaacaaa agcgtaccga
     8701 tggtcagaaa actgattttg cctaactgag atgcgttatg cagacgggca accgcgctca
     8761 gaatagaggc aaagactaac ggcataacga tcatttgcag cagttgaaca tagccgttac
     8821 caacgatgtt aaaccactgt acagaatctt taagtacctg gctgtcagaa ccataaatgg
     8881 tatgcagggc gaggccaaaa accacaccca tcaccagacc caccagcact tttttcgcca
     8941 gactccactg tttatggcgg gtctgagcca gcgcaaacag cagtacaacg aacaccacga
     9001 tgttcgcaat taatggaaag ttcatccccg ttctcctgat cttattattt ctatcactgc
     9061 gtagcagtca tagtgttggc gcaagattaa cagaactgtg agctggctct tatattcaaa
     9121 tggaattgct tattccagaa aggtgatttt tgccttttta gtattcaacc tgatgcttaa
     9181 taagccgatg aaactggatc tgcaaagtat tgatcatttg cgatgaccaa cgaacagcgc
     9241 tgttttcggg taatgaatga ggcatccata ccgaccagac aaataatgcc atgcagataa
     9301 aacgttccag ttggttgccc ttttggggaa ccaggatagg caagcggaaa cgccagcggc
     9361 atggccagag caggggaaca ccggcgggtg tcagcatatc ggcaagtatg tggctcaaat
     9421 aacccagcac catgccctgc aatgcatcag ccggaatgaa ccagctttca ggaaccttaa
     9481 ggtaaaaggt tgccagcagc gcaaataccg ccagcagact gtgggtaaaa ccacgatgcc
     9541 caaaagcgct ggcgatcggt tttgatatcc actttaatcg ctgcccaaga aacgactttg
     9601 ggtgatcgat gtccggtaac aaacacgtca ggattgcgga agggacaata tgccaccagt
     9661 caccctgtgc cagcacgggc gtcagctcgg catttttggc aaataccgca caagcaatag
     9721 aaaagagaag gtgaccttcc gccgtcatga taaaactcac taaactgtcg atacatacag
     9781 tatagggttt ttatacagtg cacggaagtg gtgacggtgt aactctttct cttaaattgg
     9841 cgattaatcc gtcagttgaa tgcaaattca gataaagaac caggggcagt gtgatcactg
     9901 ccccctggtc attaaccgag aaggtctttt gcggtgagtt ctgtcagcga tgagagtttg
     9961 acgtttgcta atacaaaacg tggatcattt tgcgcttctg gcgcaggaac gacgatggaa
    10021 cgcatgcggg ctgctttaga ggcgatcatg ccatttaccg aatcttccag tgccacgcag
    10081 gtcagagggt caacccccag ttttgctgcg cagtcgagat atacttgcgg atgcggcttg
    10141 ctgtaaggca gtttttcagc cgaggcgagg gcatcgaaac tgtcgcgtaa atcaaacata
    10201 gtcaatactt tttccagcat atgcagcggt gacgcggagg ccagccccac caataaacct
    10261 tgttctttgc ataacgcaac ggcttcacgc acacctggca ataatggacg tgtctcttca
    10321 accagtgaaa tggcacgggc aataacttgt tctactactt cctgacggct tggcccattc
    10381 catggttgcc gggcgtacca aagatcgacc accatatcga tgcgtaaacc taaggtgtcc
    10441 ggcagctcgt tacgacggga gatatccacc cccaggcttg ccatcacatc cagttcggct
    10501 cgatcccata aaggttctga gtcgataagt aatccatcca tatcaaaaat tgcagcaaga
    10561 atctgacgcg gggttgacat tacaacctct cctttagagc attatatgta aggtattaag
    10621 ccgtgcttat gattaagcgc ctatcatatc gcctttaaag aatgggcgaa aacagatatc
    10681 tacaggtaaa ctaagtgaaa cgacgcgtct taatcaagga gaactcatga cgtatcaaca
    10741 agctggacgc attgctgttt tgaaaaggat tttgggttgg gtgattttta tacctgcatt
    10801 gatctctaca ttgatttctt tgctgaaatt tatgaatacc cggcaggaaa accaggaagg
    10861 cattaatgcg gtcatgctcg actttactca tgtcatgatc gatatgatgc aggcgaatac
    10921 gccttttttg aatgtgttct ggtacaactc tccgacgccc aattttaatg gtggcgtgaa
    10981 cgttatgttc tgggtgattt tcatcctgat ttttgtcgga ctggcgttgc aggattccgg
    11041 agcccggatg agtcgccagg cgcgcttctt gcgggaaggt gttgaagatc aactcattct
    11101 ggaaaaagcc aaaggggaag aggggctaac gcgtgaacaa attgagtcac gtattgttgt
    11161 tccgcaccat actattttcc tgcagttttt ctcgctgtat atcctgccgc ttatttgtat
    11221 tgctgcaggt tatgtgttct tttctctgct tgggtttatt taatcaaaag gccgcacgga
    11281 gcggccatta tcctcaaccc atatcatgct gctaataatc tatccagtga ctgctgagca
    11341 ataaccaaat gctgaccgcc aaataacctt gcacgattta acagcgtgta gagttggtaa
    11401 accggttgac gttcaaggaa atcggcaggt agcggggata ctgactgata gccgtcatag
    11461 atttgtggcg gctgttcagt atgcagcggt aacatcgcca ggtcgcactc tcggtcaccc
    11521 cagtaacagg ccgggtcgaa aatgtacggg ccatccggac ccagcgcaca gttgccggac
    11581 cataaatcgc cgtgcaacag agaaggctgc ggttgatgcg aggcgagacg ctgctgaata
    11641 tgctcgacga tggcatcgat attgccgaaa gcgatccctt tttctgccgc cagttccagc
    11701 tgccagccaa tccgttgttc agcaaaaaaa gttgaccagc gacgttgcca ggtgttgggc
    11761 tgtggagttg tggagagcgc gttatcgaaa tcgaggccaa attgtggttg gtcgctccat
    11821 tgatgtaaac gcgcaatttg ctgaccaaga ataaatgcac tatgcgcatc cagcgggcga
    11881 ggtgggagat aatccatcac cagaaaactg tagtcacggt cagcgccaac tgcccaaacc
    11941 ttaggcacgg tgacggtttt actacgcgac agtaactcca gttggtcggc ttcggcggta
    12001 aaaccgggaa gcagttccct ttcatcacat ttgacgaaaa agtcacgtcc tgcatagcgc
    12061 aaatgccatg cggcatggac ttctccgcca ggcagttcat tacgcagttc gatttcgcct
    12121 tcacctaact gctcgctcaa aagacgactg attgcctgcc acatgatgtc tctcccatgt
    12181 tgtctgccag atcataaggt tagcgcaaga atgtggtgta aaaacacgag ctaacgcaca
    12241 ccacagactg attattgccc aaaaaccatt tattttctgt tgctattcca ttcctcccag
    12301 gtcgtgatgg tgatttcagg atctttgccg gttgcacttt gggtaagccc cgaaaccagt
    12361 tctctaattt cttcttcatt ttgggtactg ataagtccaa aagtgttagt ccccagctca
    12421 tggatattgc catcatcatc catcatggtg agcaaaaaac cgtcgcgagt caggtaatta
    12481 ttcaactcgt taatttcagt caatgtatcc tcatgcaaca tgacggttat tacataacgg
    12541 acaagatcgc cactggccat agttcacccc tttgttatca catgactttt gagcatagtc
    12601 ggagaaacgc gttgccgaca ggttggtggt gattccccca atgctggggg aatgtttttg
    12661 ttagcgggaa aggtacgcgt aaattttttg cgtatcatca tgggagcaca ggcgtgttcc
    12721 ctggttgagt gtggctgcac tccccgcagc tacgccaaaa cggaccatct cttcaagaga
    12781 ggcattttct gccagtttaa gtgtcatcgc gccgaccatg ctgtcaccag cgccaacggt
    12841 actctggctt ttcaccggtg gtggcaccac ctgaatgcag ttttcactat caacgcccag
    12901 cgccccttgt ggacccaggg aaacgacaac ccgtttggcc ttgccgctat taacgatttc
    12961 ctgcgcggct ttgcggacat cgtctggctg ggtgagttcg cgatttacca gcgcactgag
    13021 ttctttttgg ttaggcttaa ccaactcgat gttaccaatc gccagtgctg cacttaacgc
    13081 ttcgccagaa ctgtcgacga tgcagcggat cccttgtttt tgcgcagcgg aaatcagttg
    13141 ggttaatttt tccagcttca cacctggcgg caggcttccg cttatgacca ggatggcccc
    13201 ggattcaatt tcaagaactt gctcttcgag ctggcgaaac tcatcttcat ttaatgccgc
    13261 gcctggcata acaaaacgat actgctcacc gcttgcttcc aaatgtacgt gtaaattctg
    13321 ccgggtccag tctttggctt ctacagtagc gacggggaca ttttcatccg ccaacagtga
    13381 aaccagatgt tcgccggtcg cgccacccgc cgggaagatc gctgtggcag tgcctcccag
    13441 atgggcaatg gcgcgggcga cattgatgcc gccgccaccg ggttcgaaca ccggtgcggt
    13501 acagcgcagt tttccttcgg gataaatttg cggggtaatt gttgcgctat caagagaggg
    13561 cgcaagtgtc aacgtataga tacgtaccat catttcctcc tataggctga tttcagt
//
LOCUS       DADSZD010000118        13531 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.119, whole genome shotgun sequence.
ACCESSION   DADSZD010000118 DADSZD010000000
VERSION     DADSZD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.119"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            151..660
                     /locus_tag="J1Z28_003179"
     CDS             151..660
                     /locus_tag="J1Z28_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBA7717989.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            632..2560
                     /locus_tag="J1Z28_003180"
     CDS             632..2560
                     /locus_tag="J1Z28_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA7717990.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVLPMTAVEWADQYYYLPKES
                     SYGDGEWKTLPFQVAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVSCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDKPESVFYLCEH
                     HGCVIHQSEPDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVDEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDPAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2544..2750
                     /locus_tag="J1Z28_003181"
     CDS             2544..2750
                     /locus_tag="J1Z28_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-stabilizing protein"
                     /protein_id="HBA7717991.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRCRRRPLGVRL"
     gene            2747..4339
                     /locus_tag="J1Z28_003182"
     CDS             2747..4339
                     /locus_tag="J1Z28_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7717992.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNNVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4329..5834
                     /locus_tag="J1Z28_003183"
     CDS             4329..5834
                     /locus_tag="J1Z28_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBA7717993.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEVQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5871..6218
                     /locus_tag="J1Z28_003184"
     CDS             5871..6218
                     /locus_tag="J1Z28_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBA7717994.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETALTYYKSGTFATEAIHWPESVDEHKKAN
                     AFAGSALSHAALP"
     gene            6276..7304
                     /locus_tag="J1Z28_003185"
     CDS             6276..7304
                     /locus_tag="J1Z28_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBA7717995.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRMVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGIVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7356..7739
                     /locus_tag="J1Z28_003186"
     CDS             7356..7739
                     /locus_tag="J1Z28_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBA7717996.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMYGDESGAEKNYTEDAGQLNNTDDPDDVKAVRVRKCLHVMGYCPETGRPVELT
                     FRGMRVLVPSPLATAMIQHGTADHA"
     gene            7732..8085
                     /locus_tag="J1Z28_003187"
     CDS             7732..8085
                     /locus_tag="J1Z28_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717997.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGISAQFTSGTQRGGEVHGVFD
                     DPESLGFTGSGVRIEGSSPSLFVRTDMVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPVVNRRR"
     gene            8101..8634
                     /locus_tag="J1Z28_003188"
     CDS             8101..8634
                     /locus_tag="J1Z28_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717998.1"
                     /translation="MAIKGLDQAIENLSRIRKSAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERARLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKSGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKKRYPIDVVKIPMAVPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            8631..9026
                     /locus_tag="J1Z28_003189"
     CDS             8631..9026
                     /locus_tag="J1Z28_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7717999.1"
                     /translation="MKHTDIRAAVLDALEKHDTGATLFDGRPAVFEEADFPAVAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMERRIYPAMAAIPALAGMITTM
                     VQQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9034..9783
                     /locus_tag="J1Z28_003190"
     CDS             9034..9783
                     /locus_tag="J1Z28_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718000.1"
                     /translation="MATPNPLVPVKGSGTTLWVYTGKGDAYANPLSDDEWTRLAKIKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDRGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEINVTDGAAG"
     gene            9799..10227
                     /locus_tag="J1Z28_003191"
     CDS             9799..10227
                     /locus_tag="J1Z28_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA7718001.1"
                     /translation="MFLKTEQFEYNGVTLTLSELSALQRIEHLALLKQQEEQAAASGN
                     PQVSVEELVRTGAFLVAMSLWHNHPQKTQMPSMNEAVMKIEQEVLTTWPADAIARAEN
                     VVLRLSGMTGPVRMESDFTEEAENVDTDVSYSAGKPSTAS"
     gene            10254..10667
                     /locus_tag="J1Z28_003192"
     CDS             10254..10667
                     /locus_tag="J1Z28_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA7718002.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDAQLDMHFSGLTY
                     AVLSLFFCDPEMHPSDFSLLVPRREEEQTEMPDEEEILMQKAAGLAGGVRFGGEGEGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            10648..13230
                     /locus_tag="J1Z28_003193"
     CDS             10648..13230
                     /locus_tag="J1Z28_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7718003.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMTRVRRHFSGLETDARKTAGAV
                     EQSLNRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQSPWLILLQQGGQVKD
                     SFGGLIPMFRSLAGAVTLPAVGITSLVAATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAV
                     TKGFDEQTRRLKANMGTLETWADKVGSAFKSMWDAVLDIGRPESSAEMLNKAQQAFDE
                     ADKKWQWYQSRSNRRGKTSAYLSNLRGAWDDRENARLGLSAATLQADLEKASEMAARD
                     RAESEASRLKYTEEAQKAYERLQTPLDKYTARQKELNKALKDGKILQADYNTLMASAK
                     KDYESTLKKPKSSGVKVSAGERQEDQAHAALLALETELRTLEKHSGAHEKISQQRRDL
                     WKAESQFAVLAEAAQRRQLSVQEKSLLAHKDETLEYKRQLADLGDKVEHQKRLNELAQ
                     QAVRFEQQQSAKQAAIRAKARGLTDRQAKRESEEQRLRDVYRDNPQALAQVTGALKQT
                     WADEDMLRGDWLAGFKSGWGEWVESATDSFSQVKSAATQTFDGIAQNMAAMLTGSEQN
                     WRSFTRSVLSMMTEILIKQAMVGIVGSIGSAIGGAIGGAGASASTGTAIQAAAANFHF
                     ATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYADGGYVGGSGSQA
                     QMRRADGISFNQNNNVVIQNDATNGSIGPQALALVYDMARKGARDEIQAQMRDGGIFS
                     GGGR"
     gene            13227..>13531
                     /locus_tag="J1Z28_003194"
     CDS             13227..>13531
                     /locus_tag="J1Z28_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718004.1"
                     /translation="MKTFRWKVKPGMEVTSQPSVMEVRFGDGYSQRAPAGLNADLKIY
                     NVSLSVSRDDARRLEDFLAEHGGWKAFLWTPPYTYRQIKVTCAKWTTRINMLRVEFSA
                     "
BASE COUNT         3144 a         3214 c         4265 g         2908 t
ORIGIN      
        1 tccatgtccg gtttctctgt aggttaacta tatgaaaaat ataaaaacag gtcttctgtg
       61 aaccggacat gcgcaaaaaa tgggcatgta aaccggacat gaccggtttt gtcgtgattg
      121 tgaggtgaga gtttttgcga ggtgtgagga gtggctacgc agactgaagt tgccaggcat
      181 ttgagtctga ccgatcgcca gcttcgcaga ttgcagaaat taccgggtgc cccggtctcg
      241 aataagcgag ggcaactgga tctggatgcc tggcgtgatt tttacatatc gtatctgagg
      301 agaagtaaaa acgatgtgcc tgatggcgat agcgaagaag actatgaaga gaaattgctt
      361 attgccagat gggaactgac agcagaacag gccgttacac aacagctaaa aaatgaggtg
      421 tcaaaaggaa aactgatcga caccggattt tgtatttttg ccctcagtaa gctggcaatg
      481 gcgttatcca gtacgcttga ttccattccg ttatccatgc agcgacagtt cccggattta
      541 acgccacgtc atcttgacca tctgaaaacc cttattgcaa agggggcaaa tcagtgtgcg
      601 cgtgcagggg ataaattacc ggatttactc gatgaatata tcagagcaac aactgaataa
      661 tatgatggct gccgtttcgg ttgcgctgca accactggtc agggtattgc cgatgacggc
      721 agttgaatgg gctgaccagt attattatct gcctaaagag tcttcatacg gtgacggcga
      781 atggaaaacg ctgccgttcc aggtcgccat tatgaactgt atgggtaacg accaggttcg
      841 cacggttaac ctgattaaat ctgcccgtgt tggctataca aagatgttgc tgggggtggt
      901 cgggtatttt attgagcata aatcccgaaa cagcctgctt tttcagccca cggattctgc
      961 cgctgaagat tttatgaagt ctcatgtgga ggcgacgatt cgcgatgttt cctgcctgaa
     1021 aaaactgtcg ccctggctgg gacgtaaaca tcgtgacaat accctcacgc tgaaacgctt
     1081 ttcctccggt gtggggttct ggtgtctggg tggtgctgcc gccaaaaact accgtgaaaa
     1141 atccgtggat gtggtctgtt atgacgagct ttcctcgttc gaaccggatg tcgaaaaaga
     1201 gggttcgcca accctgctgg gggataaacg tattgagggc tctgtatggc caaaatccat
     1261 tcgcggctcg acgcctaaaa tcaaaggcac ctgccagatc gaaaaagcgg ccaacgagtc
     1321 ggcacatttc atgcgttttt atgtgccctg cccgcactgt ggggaggcgc agtatctgaa
     1381 atttggcgat gagtccacgc cttttggcct caaatgggag aaggataaac ctgaaagcgt
     1441 tttctacctc tgtgaacatc atggctgcgt gatccatcag tctgagcctg accagagcaa
     1501 cgggcgctgg atctgtgaaa acacgggcat gtggacccgt gacggcctga tgtttttcag
     1561 cgcccggggt gatgaaattc cgccgccgcg ctccatcacg ttccatatct ggacggcgta
     1621 cagtccgttc accacctggg tacagatagt ctatgactgg ctggatgcac tgaaagatcc
     1681 caatggcgtg aaaacctttg tgaacaccac gctgggcgag acctgggaag aggccgtggg
     1741 cgaaaaactc gatcaccagg tactgatgga taaggttgtg cgttacacgg ctgcagtgcc
     1801 tgcccgggtg gtttatctga cggcgggcat tgactcgcag cgtaaccgtt ttgagatgta
     1861 tgtctgggga tgggctccgg gagaggaagc ctttctggtg gataaaatca tcattatggg
     1921 gcgtcccgat gaggaagaga cgctgttacg tgtggatgcg gcgatcaaca aaaaataccg
     1981 tcatgcagac ggaaccgaaa tgactatttc ccgtgtctgc tgggacatcg gggggatcga
     2041 tggcgaaatc gtttatcaga ggtcaaaaaa acacggtgtt ttccgggtgc tgccggtaaa
     2101 aggcgcatct gtctatggca agccggtgat caccatgcca aaaacccgca atcagcgggg
     2161 cgtgtatctg tgtgaagtgg ggacggacac cgcaaaagaa attctctatg cccgtatgaa
     2221 agccgatccc acgcctgtgg atgaagccac gtcgtatgcc atccgttttc ctgatgatcc
     2281 ggagattttt tcgcagacag aggcgcagca actggtcgcg gaagagcttg tggagaagtg
     2341 ggaaaaagga aagatgcgtc tgctgtggga taacaaaaag cggcgtaacg aagcgctgga
     2401 ctgcctggtg tatgcctacg cggcattacg tgtgtccgtg caacgctggc agcttgatcc
     2461 ggctgtactg gcaaaatccc gggaagaaga gacgacccgg ccaactctta aagaactggc
     2521 agcgaagctg tccggaggag tgaatggtta cagtcgctga actgcaggcg ctgcgtcagg
     2581 cgcgccttga tttattaacc ggtaaacggg tggtgtctgt ccagaaagat ggtcgcagaa
     2641 ttgaatatac ggcggcttct ctggatgagc ttaaccgggc gatcaatgat gcggagtcgg
     2701 tactggggac aacccggtgt cgccgtcgtc cgctgggagt gagattatga aacgaacgcc
     2761 tgtcctgatt gatgtgaacg gcgttccgct tcgtgagagt ctcagctaca acgggggcgg
     2821 tgcaggattt ggcgggcaaa tggctgagtg gttgccaccg gcgcagagtg ccgatgcggc
     2881 cctgctgccc gcgttgcgtc tggggaatgc ccgggcagat gatctggtgc gcaataacgg
     2941 aatagcggct aatgcggtgg ctctgcataa ggatcacatt gtcgggcata tgtttctgat
     3001 cagctaccgt ccgaactggc gctggctggg gatgcgggag accgcagcaa aaagctttgt
     3061 cgatgaggtg gaggcggcct ggtcggaata cgccgaaggg atgtttggcg agatcgacgt
     3121 ggaaggaaaa cgcacgttca cggaatttat ccgtgaaggt gtgggcgttc atgcgtttaa
     3181 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgtacgcg
     3241 ttttaaagcc gtgagtccga aacgggtgga cacgcctgga cacggtatgg ggaaccgttt
     3301 tctgcgggcc ggtgtggagg tcgatcgata tggccgtgcc gtcgcgtacc atatctgtga
     3361 ggatgatttt ccgttctctg gtagtggacg atgggaacgg atcccgcgtg aacttcccac
     3421 cgggcgtccg gccatgctgc atattttcga gccggtggag gacgggcaga cccgtggggc
     3481 taatcagttt tacagcgtca tggaacggct gaagatgctc gattccctgc aggcaacaca
     3541 gcttcagtcg gccatagtga aggcgatgta tgcagcgacg attgaaagtg aacttgatac
     3601 cgaaaaggcc tttgaatata tcgccggcgc gccacaggag cagaaggata atccgcttat
     3661 taatattctg gagaagtttt ccagctggta tgacacgaat aacgtgacac tgggcggtgt
     3721 caaaattccg caccttttcc ctggtgatga tctgaaacta cagactgcgc aggattcaga
     3781 caatggattt tctgcgcttg aacaggctct gctgcggtat atcgccgccg gtcttggcgt
     3841 ttcctacgaa cagttgtccc gtgattactc gaaggtcagt tactcaagtg cccgcgcctc
     3901 cgccaatgag tcgtggcgct attttatggg gcggcgaaaa tttattgcgg cccggctggc
     3961 cacgcagatg ttttcctgct ggctggaaga ggcacttctt cgggggatta ttcgtccgcc
     4021 acgggcacgt tttgattttt atcaggcgcg atcagcctgg tcacgggcag agtggattgg
     4081 tgccggaaga atggccattg acgggctcaa ggaagtccag gaatcagtga tgcgcattga
     4141 ggccggactg agcacgtatg agaaagagct ggcgctgatg ggcgaggatt atcaggacat
     4201 tttccgccag caggtcaggg aatctgctga gcggcaaaaa gccggactct cacgtccggt
     4261 gtggatagcg caggcgtatc agcagcagat agcggagagt cgcaggccgg aagaggagac
     4321 aacaccacgt gagacgtaat ctttcacaca ttattgccgc agcattcaat gaaccgctgc
     4381 ttctggagcc cgcctatgcg cgggttttct tttgcgcgct cgggcgcgag atgggggcag
     4441 caagtctttc ggtaccacaa cagcaggtac agtttgatgc tcccggaatg ctggctgaaa
     4501 cggacgagta catggccgga ggtaaacgac cggcccgtgt ttacagggtg gtgaacggta
     4561 ttgctgtact gccggtgacc ggcacgctgg tgcaccggct gggtggtatg cggccatttt
     4621 ccggaatgac aggctatgac ggtattgtcg cctgtcttca gcaggcaatg gcggatagcc
     4681 aggtgcgggg cgtactgctg gacattgaca gtccgggcgg gcaggccgcc ggcgcgtttg
     4741 actgcgctga catgatttac cgcctccgtc agcagaagcc ggtctgggca ctgtgcaatg
     4801 acacggcctg ttctgcagcc atgctgctgg cgtcggcctg ctcccgacgg ctggttaccc
     4861 agacatcccg tatcggttcc attggcgtga tgatgagcca tgtcagctat gccggtcatc
     4921 tggcgcaggc cggtgtggat atcacgctga tttactcagg ggcgcacaag gtggatggca
     4981 atcagtttga agcgttgccg gcagaggttc gccaggacat gcagcagcgg attgatgcgg
     5041 cgcgccggat gtttgccgaa aaagtggcga tgtttaccgg tctgtctgtt gatgcagtca
     5101 cgggaacaga ggccgctgtt tttgaaggtc agtccggcat tgaggccggg ctggcggatg
     5161 aattaatcaa tgcgtcggat gccatcagtg tgatggccac ggcgctgaac agtaatgtca
     5221 gaggaggcac tatgccgcaa ttaactgcaa cggaagccgc cgcgcaggag aaccagcgag
     5281 tgatggggat cctgacatgc caggaagcga aaggacgtga acagcttgcc acgatgctgg
     5341 caggacaaca gggcatgagc gttgaacagg cccgggcgat tctggccgcg gcggcaccgc
     5401 agcagccggt ggcatccacg cagagtgaag ccgatcgcat tatggcgtgt gaagaagcga
     5461 acggtcgtga acaactggcg gcaacgctgg cggcgatgcc ggagatgacg gtggaaaaag
     5521 cccgcccgat cctggctgct tcaccgcagg cggatgccgg accatcactc cgtgatcaga
     5581 tcatggcact ggatgaggca aaaggggctg aggtgcaggc tgaacagctg gctgcctgcc
     5641 cgggaatgac tgtggagagc gcccgggctg tgctggctgc gggatcaggt aaggcagaac
     5701 cggtctctgc atccacaacc gccatgtttg aacatttcat ggcgaaccat tcaccggcag
     5761 cggtacaggg tggcgtgcca cagacgtcag cagacggtga tgcggacgtg aaaatgctca
     5821 tggccatgcc atgaagccag tgctgaccat caacaggagg tttttacaat atggtgacga
     5881 aaaccatcac tgaacagcgt gcggaagtac gtatttttgc cggtaatgat ccggctcata
     5941 ccgccacagg cagcagcggg atttcctcgg caacaccggc actgacgccc ctgatgctgg
     6001 atgaagccac cgggaaactg gtggtctggg acggacagaa agccggtagt gcggttggca
     6061 tactggtact gccgcttgaa ggcacagaga cggcgctgac gtattacaag tcgggaacct
     6121 ttgcgacgga ggcaatccac tggcctgaaa gtgtggatga acacaaaaag gccaacgcct
     6181 ttgccggcag tgccctgagt cacgcggcgc tgccgtaaca cgttatcagg ccaccgcggt
     6241 ggcctgactg atttctgaat gaaaggaact gatttatggg attgtttacg acccgccagt
     6301 tactcggtta taccgaacaa aaagttaaat ttcgtgcgct gtttctggag ctgtttttcc
     6361 gccgtatggt gaatttccat accgaagaag tgatgctgga caaaattacc ggaaaaacgc
     6421 cggtggcggc ctatgtctcc ccggttgttg aaggaaaagt gctgcgtcat cgtggtggtg
     6481 aaacccgcgt gttacgtccg ggctacgtca agccgaaaca cgaatttaat taccagcagg
     6541 cggttgagcg tcttcccggt gaagatccgg ctcagctgaa cgacccggcc taccgccgtc
     6601 tgcgtatcat taccgataac ctcaaacagg aagagcacgc gattgtccag gtggaagaaa
     6661 tgcaggcggt gaatgccgtg ctgtatggca aatacacgat ggaaggggag cagtttgata
     6721 ctgtcgaggt ggatttcggg cgctctgaag gaaataacat tgagcaggct gacggtaaaa
     6781 aatggtctga gcaggaccgt gatacgtttg atccgacgca tgatattgac ctctactgcg
     6841 atcaggccag cggtcttgtg aatattgcca tcatggacgg tatcgtctgg cgtctgctga
     6901 atggctttaa actgttccgc gaaaaactgg atacccgtcg cggctcaaat tcacaactcg
     6961 aaacggcagt gaaagatctg ggcgcagtgg tgtccttcaa ggggtattac ggcgatctgg
     7021 ccattgtggt ggcgaaaacg tcttatgtgg cagaggacgg taccgaaaaa cgttatctgc
     7081 cggagggcac actggtcctg gggaatacgg cagcagaggg cattcgttgc tatggtgcca
     7141 ttcaggatgc gcaggcgttg tctgagggtg tggtggcctc ttcccgttat ccgaaacact
     7201 ggctgaccgt gggcgatccg gcccgtgaat ttaccatgac gcagtctgcg ccgctgatgg
     7261 tgttgccgga cccggatgag tttgtggtgg tacaggtgaa ataatccgtg agcgggggcg
     7321 aaatgccccc gtgtctttat tcacaggaga ctgagatggc aacaaaagaa gaaaatctga
     7381 atcgtcttcg tcaactggct ggcctgctgg ggcgcgaggc ggatatgtcg gggagtgctg
     7441 cggatattgc gcaacgtgtg tctgagtggg aagaggagct tgctgcttcc cgggagggca
     7501 ttatgtacgg cgatgagagc ggggctgaga aaaattacac agaggatgcc gggcagttga
     7561 acaacactga tgatccggat gatgttaaag ctgtccgtgt gcgcaagtgc ctgcatgtga
     7621 tggggtattg cccggagaca ggccgtcctg ttgagctgac gttccggggg atgcgtgttc
     7681 tggtgccatc accactggcg acagccatga tacagcacgg aacggctgat catgcgtgac
     7741 tttcagaatg cctttgatgc cgctcttgcc ggggtggaca gcacaattgt tgaagtgatg
     7801 ggtatcagtg cgcagttcac ctccggtaca cagcgtggcg gggaagttca tggcgttttt
     7861 gacgatccgg agtcgctggg ttttaccggc agtggggtcc gtattgaagg aagcagcccg
     7921 tcattatttg tgcggacgga tatggttcgt gctgtgcggc gtggtgacac gctgaccatt
     7981 aacggcgaga tgttctgggt ggatcgtgtt tctccggatg acgggggcag ctgttatctc
     8041 tggctcaacc gtgggcaacc acccgtagtt aaccggcgac gataaacgca gggggaaatg
     8101 atggcgataa aagggcttga tcaggcgatt gaaaatctga gccggattcg taaaagcgcc
     8161 attccggctg cttcagcaat ggccattaac cgcgtggcca caacggcgat taatcagtct
     8221 tcgtcacagg tcgcccggga gacccgggtg agccggaaac tggttaaaga gcgtgccaga
     8281 ctgaaacggg ctacggtcag aaatccgaat gcaaaaatta tcgttaaccg cggtgatctt
     8341 cctgtgatta agctggggat cagaatgctg gggcgtcgtc cgaacagcat actcaaatcc
     8401 ggtcagcatc gttatcagcg ggcatttatc cagcgattaa aaaacggtcg ctggcatgtc
     8461 atgcagcgtg tggccgggaa aaaacgttac cccattgatg tggtgaaaat cccgatggcg
     8521 gtcccactga aacaggcttt tgatgagaat gttgaccgta tccggcgtga acgcctgccc
     8581 aaagaactgg catatgcgct gaaacaacaa ctgaggattg cgataaaacg atgaaacata
     8641 ctgatatccg tgcggcagtg ctggatgcac tggagaagca tgacaccggg gcgacgctgt
     8701 ttgatggtcg ccccgctgtt tttgaggagg cggattttcc ggcggtcgcg gtttatctga
     8761 cggatgcaga gtataccggt gaagagctgg atgcagatac ctggcgggcc acactgcata
     8821 ttgaggtgtt tttaccggca caggtaccgg attcagagct tgatcagtgg atggaaaggc
     8881 ggatttaccc ggcgatggcg gcgatccctg cactggcggg catgattacc acgatggtgc
     8941 agcagggcta tgagtatcgt cgcgatgacg atatggcgtt atggagttct gctgatctga
     9001 cgtattccat tacctacgaa atgtgaggat gatatggcaa caccaaatcc cctggtgccg
     9061 gtaaaaggtt ccggcaccac gctctgggtt tacaccggta agggcgatgc ttatgcaaac
     9121 ccgctgtcag atgatgagtg gactcgcctg gcaaaaataa aggatctgac ccccggcgag
     9181 atgacggcag aatcctacga cgataactat ctggatgatg aggatgctga ctgggtatcc
     9241 accgggcagg ggcagaaatc tgcaggtgac accagtttta cgctggcctg gaagcccggc
     9301 gagaaagggc agcgtgattt gattgcctgg tttgacagca gcgagacccg ggcctacaaa
     9361 atccgcttcc cgaacggtac ggtggatgtg ttccgtggct gggtgagcgc cattggtaaa
     9421 gcggtgaccg ccaaagaggt gatcacccgt acggtaaaaa ttaccaatat cggtcgccct
     9481 tcgctggcgg aggatcgggg ggacatcaca ccggtcaccg gtattaccgt gacgccacca
     9541 acgggcaatg tggcaaaagg tcagaatatc accctgaccg tggccgttca gccggaaggc
     9601 gcaacggata aaacgttccg cgccacgtca gcgaatcaga atttcgcgac cattaccgtg
     9661 aaagggaaca cgatcacggt gaaaggtgtt gcggcaggta aagcgcagat ccctgttgtc
     9721 accggcaatg gtgagtttgc tgcagttgct gaaattaacg tcacggatgg tgccgctggc
     9781 tgaggtgaga gataaagcat gtttctgaaa acagaacaat ttgaatataa cggtgtgacc
     9841 ctcacgctgt cggagctgtc cgcgctgcag cgtattgagc atctggcttt gctgaaacag
     9901 caggaagaac aggccgccgc cagtggcaac ccgcaggtga gcgtggaaga gctggtcaga
     9961 accggcgctt ttctggtggc gatgtccctg tggcataacc atccacagaa aacgcagatg
    10021 ccgtccatga atgaggccgt gatgaagatt gagcaggaag tgctcaccac ctggcctgcc
    10081 gatgccattg cccgggctga aaatgtggta ttgcgtctgt ccgggatgac cgggcctgtc
    10141 cggatggaaa gtgatttcac ggaagaggca gaaaatgttg atactgacgt ttcttattcc
    10201 gcgggaaagc cttcgacggc gagctgaatt ttgccctcag actggcgcgt gagatgggga
    10261 ggcctgactg gcgcgccatg cttgccggga tgacatccac cgaatatgcc gactggcgac
    10321 gtttttaccg cacgcattat tttcaggatg cccaactgga tatgcatttt tccgggctga
    10381 cgtacgccgt actcagcctg tttttttgcg atccagaaat gcatccctcg gatttcagtc
    10441 tgcttgtccc ccggcgagag gaagagcaga cggagatgcc ggatgaggaa gaaattctga
    10501 tgcagaaagc ggcaggactt gccggaggcg tccggtttgg tggggagggt gagggcgata
    10561 tttcaccttc tgcggatgtg gtggatgtca gcgaggatga tgttgcatta atgatggctt
    10621 cagcggggat ttccggaggt gtgagatatg tcccagccgg ttggtgatct tgttattgac
    10681 ctgagtctgg atgcggtccg tttcgatgag cagatgaccc gcgtaaggcg tcatttttca
    10741 ggactggaga ctgacgccag aaaaaccgcc ggtgccgttg agcagagtct gaaccgtcag
    10801 gcgctggccg cacaaaaagc cgggatttcc gtcgggcagt ataaagccgc catgcgtacc
    10861 ctgcccgcac agttcacgga tatcgccacg cagcttgccg gtggtcagag tccgtggttg
    10921 atcctgctgc agcagggcgg acaggtgaag gactccttcg gcgggctgat ccccatgttc
    10981 cggagccttg ctggtgccgt cacgctacct gctgtcggga tcacatcact cgttgcagca
    11041 accggggcgc tggcgtatgc ctggtaccag ggggactcca cgctttcagc gtttaataaa
    11101 accctggttc tttccggtaa tcagtccggg ctgaccgcag atcgcatgct gactctctcc
    11161 agagcaggac aggcagcagg gctgacgttt aatcaggcga gtgagtcact ggcagcactg
    11221 gtgaacgccg gtgtgcgtgg tggtgaacag tttgatgcca ttaatcagag tgtggcgcgt
    11281 tttgcttctg catccggtgt ggaagtggac aaggttgcag aggctttcgg aaaactgacc
    11341 accgacccga cgtcggggct gatggcgatg gcgcgccagt tccgtaacgt gacggcagag
    11401 cagattgcgt atgttgcgca actgcagcgt tcgggtgatg aggccggggc attgcaggcc
    11461 gcaaacgagg ccgtcacaaa agggtttgat gagcagaccc gccgcctgaa agcgaacatg
    11521 ggaacgctgg agacctgggc ggataaggtc ggcagtgcgt ttaaatccat gtgggatgca
    11581 gtgctggata tcgggcgtcc tgagtcatct gcagaaatgc tgaataaggc acagcaggca
    11641 ttcgatgagg cggataaaaa atggcaatgg tatcagagtc gcagtaaccg gcgcggaaaa
    11701 acgtcagcgt atctttctaa tctgcgcggc gcatgggacg accgcgaaaa cgcccgcctc
    11761 ggtctttcag cggcgacact gcaggcagac ctggaaaaag ccagcgaaat ggcggcacgg
    11821 gatcgggctg aatcagaggc ctcacggctg aagtacaccg aagaggcgca gaaggcatat
    11881 gaacgcctgc agacgccgct ggataaatat accgcccgcc agaaggaact gaataaggca
    11941 ctgaaagacg ggaaaatcct gcaggcagat tacaacacgc tgatggcgtc ggcgaaaaag
    12001 gactatgagt cgacgctgaa aaaaccgaag tcgtcaggtg taaaagtgtc agccggtgaa
    12061 cgtcaggaag accaggcgca tgctgccctg ctggcgcttg aaaccgagct ccggacgctg
    12121 gaaaaacaca gcggtgcgca tgagaaaatc agccagcagc gccgggattt atggaaggcg
    12181 gaaagtcagt tcgcggtact ggcggaggcg gcacaacgtc gccagctgtc cgtacaggag
    12241 aaatccctgc tggcgcataa agatgagacg ctggaataca aacgccagct ggctgaccta
    12301 ggcgacaagg ttgaacacca gaaacgcctg aatgagctgg cacagcaggc ggtgcggttt
    12361 gaacagcagc agagcgcgaa gcaggcagca atcagagcaa aagcccgcgg tctcaccgat
    12421 cgtcaggcga agcgtgagtc tgaagagcag cgtcttcgtg acgtgtaccg cgataatccg
    12481 caggcgctgg cacaggtcac cggggcactg aaacagacat gggcggatga agacatgctg
    12541 cgcggtgact ggctggccgg gtttaagtct ggctggggtg agtgggtgga aagtgcgacg
    12601 gacagttttt cgcaggttaa aagtgctgca acgcagacct ttgatggtat tgcacagaat
    12661 atggcggcaa tgctgaccgg cagtgaacag aactggcgca gtttcacccg ttcggtgctg
    12721 tccatgatga cggaaatcct gattaaacag gccatggtgg ggattgtcgg gagtatcggc
    12781 agcgccatcg ggggagccat tggtggtgct ggcgcatcag cttccacggg gacagccatt
    12841 caggctgcgg cagcgaactt tcatttcgcg accggtggat tcaccggcac tggcggaaaa
    12901 tatgagcctg cggggattgt tcaccgtggt gagtttgtgt tcacgaagga ggcaaccagc
    12961 cggattggtg tcggcaacct gtaccgcctg atgcgggggt atgcggatgg cggttatgtg
    13021 ggtggttccg gaagccaggc tcagatgcgg cgggcagatg gcataagttt taatcagaac
    13081 aacaatgtgg tgattcagaa tgatgccacg aacggcagta tcggtccgca ggcactggcg
    13141 ctggtttatg acatggcccg taagggagcc cgtgatgaga ttcaggcaca gatgcgtgat
    13201 ggtggtatat tctccggagg tgggcgttga aaacatttcg ctggaaagtg aaaccgggaa
    13261 tggaagtgac ctctcaaccg tcggttatgg aagtgcgttt tggggacggg tattcccagc
    13321 gtgcgcctgc cgggctgaat gcggatctga aaatctacaa cgtttcgctg tctgtgtccc
    13381 gtgatgatgc cagacggctg gaagattttc tggcagagca cgggggctgg aaggcgtttc
    13441 tgtggacgcc gccttatacg tacaggcaga taaaggtgac gtgtgctaaa tggaccacca
    13501 ggataaatat gctgcgtgtg gaatttagtg c
//
LOCUS       DADSZD010000119        13524 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.120, whole genome shotgun sequence.
ACCESSION   DADSZD010000119 DADSZD010000000
VERSION     DADSZD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.120"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            12..539
                     /locus_tag="J1Z28_003195"
     CDS             12..539
                     /locus_tag="J1Z28_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBA7718005.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATV
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            548..2647
                     /locus_tag="J1Z28_003196"
     CDS             548..2647
                     /locus_tag="J1Z28_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBA7718006.1"
                     /translation="MGFSSARNLGRDISAGFSPPRRMPISEAVKKFMRVPKGAGNSVP
                     WDPELTPYIIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWIVYTIVCDPSDMLVVQ
                     MTEDKAREHSKKRLDRTFRSSAAVKKRMSPRRNDNNVHDKTFRDGSFLKIGWPSVNIM
                     SSSDYRFVALTDYDRFPENIDSEGDGFSLASKRTTTFMSAGMTLVESSPGRDICDSKW
                     RRKSPHEAPPTTGILSLYNRGDRRRWYWSCPHCGEYFQPAMDAMTGYRNEPDPFKASE
                     AAYLLCPHCSGIITAEKKRELNSAGVWLREGQVIDRNGNVSGEPRRSRIASFWMEGPA
                     AAYQTWAQLVYKLLTAEQEYEATGSEETLRAVINTDWGLPYLPRASMEQRKSELLEQR
                     AEPVPSRSVPDGINFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRSD
                     CDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDNA
                     YKFWRRCRRDGLGKRIYLFKGDSIRRAKLISRTFPDNTGRTGRRAQAAGDVPLWLLQT
                     DALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAFD
                     LMVYAEALVILHGYEKIRWPDAPEWASRETWLECVQDSTEPSSSPEPVSTPVKKQKRK
                     KTVTDDVNPWLTSGGWL"
     gene            2644..2856
                     /locus_tag="J1Z28_003197"
     CDS             2644..2856
                     /locus_tag="J1Z28_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718007.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            2856..4364
                     /locus_tag="J1Z28_003198"
     CDS             2856..4364
                     /locus_tag="J1Z28_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7718008.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLTP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEADGNGSKDKERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            4309..6336
                     /locus_tag="J1Z28_003199"
     CDS             4309..6336
                     /locus_tag="J1Z28_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBA7718009.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDGPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQSLQAQCLADPECSLEQAREKLLSEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARRLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            6423..6746
                     /locus_tag="J1Z28_003200"
     CDS             6423..6746
                     /locus_tag="J1Z28_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBA7718010.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            6739..7014
                     /locus_tag="J1Z28_003201"
     CDS             6739..7014
                     /locus_tag="J1Z28_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HBA7718011.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            7026..7604
                     /locus_tag="J1Z28_003202"
     CDS             7026..7604
                     /locus_tag="J1Z28_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718012.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVTTRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTAGVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            7601..8002
                     /locus_tag="J1Z28_003203"
     CDS             7601..8002
                     /locus_tag="J1Z28_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718013.1"
                     /translation="MNRHTQIRQVVLARLREQCGDSATFFDGLPAFVDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDVPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            8013..8756
                     /locus_tag="J1Z28_003204"
     CDS             8013..8756
                     /locus_tag="J1Z28_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718014.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVAAVTVSAAG"
     gene            8817..9203
                     /locus_tag="J1Z28_003205"
     CDS             8817..9203
                     /locus_tag="J1Z28_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBA7718015.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLYASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAGDLEHEVLTPEKS"
     gene            9212..9541
                     /locus_tag="J1Z28_003206"
     CDS             9212..9541
                     /locus_tag="J1Z28_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBA7718016.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            9513..12476
                     /locus_tag="J1Z28_003207"
     CDS             9513..12476
                     /locus_tag="J1Z28_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBA7718017.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQAGKATHSLGLKSAAARQEIGV
                     LIGELARGNLGALRGSGITLANRAGWIDTLMSPKGMMLGGVIGGIATSVYGLGKAWYD
                     GQKEGEEFNRQLSLTGHYAGVTAGQLWTLSRAISGNGITQHAAAGVLAQVVGSGAFRG
                     NDIGMVARAAAQMERSVGQSVSDTINQFKRLKDDPVNAAKALDNELHFLTATQLEQIR
                     VLGDQGRSSDAARIAMSALAEETGRRTADIDNNLNALGSTLRYLSDLWSRFWDAAMNI
                     GREDSLDEQIAALQEKVSRAKRLPWTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQA
                     ERNYQEQQKRRNAENAALNRMNETEAARHQREIARINSMQYADQAVRDAAIQRENERY
                     EKALASGKKKTRETRNDEATRLLLQYSQQQAQVEGQIAAARQSAGIATEKMTEAHKQL
                     LALQQRISDLDGKKLTADEKSVLARKDELIQALTLLDVKQQELQKQTALNDLKKKTIQ
                     LTSQLAEEELAQRQQHDLDIATVGMGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKG
                     TYNTDDYRKAEQALTESLNRQLNENRRYWQQLEVMQGNWKNGVLRAFQDFTVDADNTA
                     GTAEQVFSSAFSNMGNGLATFVTTGKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGS
                     VLGFDLSSLSLNANGGIYQSADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLR
                     RGADGKLGVVADIGGSGMAMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFM
                     QQQARDGGRLSGAYR"
     gene            12476..12805
                     /locus_tag="J1Z28_003208"
     CDS             12476..12805
                     /locus_tag="J1Z28_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718018.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            12815..13513
                     /locus_tag="J1Z28_003209"
     CDS             12815..13513
                     /locus_tag="J1Z28_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA7718019.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTVVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYLIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
BASE COUNT         3292 a         3135 c         4102 g         2995 t
ORIGIN      
        1 tggagttgct tatgtcaaat gtgagcggga tcggtgatgc ttattactgg agtgttttta
       61 aaatcgctga ggcctttggg cttcaccggg acacagtaaa aaaacggctt ctcgcggcca
      121 acactcctgt ggctgcgact gtcaggggga accccgttta cgccctgcag catgtcgggc
      181 cagccctgtt tagtgtgaag catgaggcag cagactctgt tcatgatcca tcccgtatgg
      241 agccgaaaga gagaaaggac tggtaccagt ctgaaaatga aaggatcaag ctggaaaagg
      301 agcagcgaaa actcatcccc gttgatgaag tagtcatcgt ctattcgtcc atgagaaagg
      361 ctgtcgtcca ggttctggag acaattccgg atgttcttga acgcgattgc gccctgactc
      421 ctcaggccgt cggcgttgta cagcaggcaa ttgatgacct gcgatacact cttcaggaaa
      481 aatcctacga ggcttgtgct gctgaattaa ttcctgatga ggaaggagag agtctctagg
      541 aggaataatg ggtttttcat cagcccgaaa tttgggaagg gacatatcgg caggattttc
      601 cccaccacgt cgcatgccga tttcggaggc tgttaaaaaa ttcatgcgtg ttcccaaggg
      661 ggctggtaac tcggtgccat gggatcctga actgacaccc tacatcattg agcccatgaa
      721 ctgcctggca tcgcgtgaat acgatgcggt gatttttgtt ggtcctgcgc gaacagggaa
      781 gaccattggt ctgatcgatg gatggattgt ctataccatc gtttgcgatc cttcggacat
      841 gctcgttgtg cagatgaccg aagataaggc ccgcgagcat tctaaaaagc gcctcgacag
      901 aacgtttaga agcagtgcgg cggtaaagaa aagaatgagt ccacgtcgta acgacaataa
      961 tgtccatgat aagacgttca gggatggctc gttccttaaa attggttggc cctcggtcaa
     1021 cattatgtcg tcgtcggatt accggtttgt cgccttaacc gattacgacc gttttccgga
     1081 gaatatcgac agcgagggtg atggtttctc cctggcctca aaacgtacca ccacatttat
     1141 gtccgccggg atgactctgg tggagagctc gccgggacgt gacatctgcg acagcaaatg
     1201 gcgacgtaag tcgcctcatg aagcgccacc gacgactggt attctttccc tttacaatcg
     1261 tggtgaccgc cgccgctggt actggtcatg tccgcactgc ggtgaatatt ttcagccagc
     1321 catggatgcc atgaccggct accgtaatga accggatccc tttaaagcca gtgaggcggc
     1381 gtatctactt tgcccgcact gcagcggcat tatcactgcg gagaaaaagc gtgagctcaa
     1441 tagtgcagga gtctggttgc gtgaaggtca ggtcattgat cgtaacggca acgtttccgg
     1501 tgaaccgcgc cgctcccgta tcgccagttt ctggatggaa gggccagctg ctgcgtatca
     1561 gacctgggcg caactggttt acaaattact gactgcagaa caggagtatg aagcgacagg
     1621 aagcgaagaa acactcaggg cggttatcaa caccgactgg ggattgcctt atcttcctcg
     1681 tgccagcatg gagcaacgaa aaagtgaact gcttgagcag cgggcagagc cagttccttc
     1741 ccgcagtgtg ccggatggca ttaatttcct tgtggcgaca gtggatgtgc aggcgggacg
     1801 tcatcgccgt tttgtggttc aggtaacggg ctatggcagt cgtggcgaac gctggattat
     1861 tgatcgttac aacatcacgc agtcattgcg cagtgactgc gacggggaga gccagcgaat
     1921 tgatccggcc agctatccgg aagactggga tgtcctgctg acggatgttt ttcataaaag
     1981 ctggccgctg gcctccgatc cttctcaaca aatgcgactg atggcaatgg cggtggactc
     2041 cggcggtgaa gacggggtca ctgataatgc ctataaattc tggcgtcgtt gccgtcgtga
     2101 tggccttggt aaacgtattt acctgtttaa gggggacagc atccggcgcg caaaactgat
     2161 cagccgtaca ttccctgata acaccggacg aacgggccgc cgggcgcagg ccgcaggtga
     2221 tgtgccgctc tggcttcttc agacggatgc cctgaaagac cgggtgaata acgcgttatg
     2281 gcgtgactcg ccaggtccag gctatgtgca tttccctgac tggctgggga gctggtttta
     2341 cgacgaactg acgtatgaag agcggagcag tgacgggaaa tggagtaagc cgggtcgcgg
     2401 tgccaacgaa gcttttgacc tgatggtgta tgccgaggct ctggtcattc tgcatggata
     2461 cgaaaagatc cgctggccgg atgcaccgga gtgggcgagc cgggaaacct ggctggagtg
     2521 tgtccaggac agtaccgaac cgtcatcctc accggaaccg gtatccacgc ctgttaaaaa
     2581 acaaaaacgg aagaaaacag taactgacga tgttaacccc tggctgactt ctggaggatg
     2641 gttatgaacc agaatgatat cgaagccatg attcagcgtt atacggaagc tgaaatggcg
     2701 gtgctggacg gaaaatccgt cacttttaat ggtcagcaga tgaccatgga aaacttatct
     2761 gagatccggc aggggcggca ggagtgggag cgccgccttg cggctctgat tacacgacga
     2821 cgggggcatc ccgggtaccg gctggcgagg ttctgatggc aattcttgat gatgtgattg
     2881 gcgttttttc accaggatgg aaagcggcaa ggctgcgttc ccgtgcggtg atccaggctt
     2941 atgaggccgt aaaaacgacg cggacacaca aagcccgacg ggagaaccga actgccgacc
     3001 agttaagcca gtacggggcc gtgtcgttac gtgagcaggc ccgttacctt gataacaacc
     3061 acgatctggt cattggtgta tttgacaagc tggaagaacg ggtggtgggg aaaaacggga
     3121 ttattgtcga gccacatccg gtattacgca atggggccat tgcccgtgat ctggcagcgg
     3181 agatacgcac ccgatggagt gaatggtctg tcagtccgga agtcaccggg cagtttaccc
     3241 gtccgatgct ggaacgtctg atgctgcgta cctggctgcg cgatggtgag gtgtttgccc
     3301 agatggtttc cgggcgcata aacagcctga cgccttctgc cggtgttcat ttctggctgg
     3361 aggcgctcga gccagacttt attcccatga ccagtgatga gagcaacagg ctgaatcagg
     3421 gcgtgtttgt tgatgactgg gggcgtcccg aaaaatatct ggtgtataaa agtcgtcctg
     3481 tatccggacg gcagatggaa accaaagaag tggatgcaga gcgaatgctg catcttaaat
     3541 ttgttcgccg tctgcaccag atgcgcggga cgtctttgtt gtccggtgtg ctgatccgcc
     3601 tcagtgccct gaaagagtat gaagattctg agctgactgc agcaaggatc gccgctgctc
     3661 tggggatgta catccggaaa ggcgacgggc agagctatga agcggatggt aatggcagca
     3721 aggataagga acgcgagctt accattcagc caggcattat ttacgatgat ctgaaacccg
     3781 gcgaagaaat cggaatggtg aagtcggatc gccccaatcc taaccttgaa acttttcgta
     3841 atggtcagtt gcgtgccgtg gcggcgggca gtcgtctgag tttttccagt acagcgcgca
     3901 actataacgg cacttacagc gcccagcgtc aggagctggt tgaatccact gatggctacc
     3961 tgatcctgca ggactggttt attggtgccg tcacccgccc gatgtatcgt gcctggctga
     4021 aacaggctgt ggcatccggt gttatcaggc taccccgcga tcttgaccgt tcttcactgt
     4081 ataccgcggt gtattccgga ccggtgatgc cgtggattga ccctgttaag gaggctgagg
     4141 cctggaaaat ccagattcgt ggtggagcgg cgacagaatc agactgggta cgtgctggtg
     4201 gtcgtaatcc ggatgatgtc aaacgtcggc gcaaggccga aattgatgaa aaccgcaagc
     4261 tggatctggt atttgatacc gatccggcca gtgataaagg aggcagcagt gccgcaacga
     4321 aacgacagga gccgcagcac accgacgacc agtccgaaga ataattcctg gttcaggatg
     4381 caggctggtc accagagtga cgcggatatt tatatttatg acgagattgg tttctggggt
     4441 gttacagcga agcagtttat cagtgatctg aatgcactgg gcgatatcac ccacattaat
     4501 ctccatatta attcaccggg tggcgatgtc tttgaaggca tcgccatttt taatgcgctg
     4561 aaaacacatg gtgcgtccat taccgtttat gtcgacggtg tggcggcgtc aatggcgtcg
     4621 gtcattgcga tggtgggaaa cccggtcatt atgccggaaa acaccttcat gatgattcat
     4681 aaaccatttg gctttacggg cggtgatgcg gaggacatgc gcacctatgc cgacctgctc
     4741 gataaagttg aggcggttct gttacccgct tatgcacaga aaaccgggaa aaccaccgat
     4801 gaaattgctg ccatgctggc ggatgagacc tggatgtccg gtgccgaatg tctggcacat
     4861 ggatttgctg atcaggtgac accagccgtt aaggcaatgg catgtattca gtcaaaacgt
     4921 acagaggaat ttaaaaagat gccggaatcc attcgaaata tgattactcc gccacgcaac
     4981 agtgctccac gcgtacagga tgatggacct gcagcctccc ggacgccagt gcaggcagca
     5041 gcacccgtgg tggatgaaaa cagcatccgt gcgcaggtcc tggcagagca aaaagcgcgt
     5101 gtaaacggta ttaatgatct gtttgccatg tttggcgggc gttatcagtc gttgcaggct
     5161 cagtgtcttg ccgatcctga atgttcgctg gagcaggccc gcgaaaagct gttgagcgag
     5221 atggggcgcg agtccacgcc atccaataaa aataccccgg ctcatattta tgccggtaac
     5281 ggtaattttg tgggggacgg gatccgccag gcgctgatgg cgcgcgccgg gtttgaaaaa
     5341 accgaacgtg ataatgtcta caacgggatg accctgcgtg aatatgcccg tatgtcacta
     5401 actgaacgtg gtattggtgt ttccagttat aacccgatgc agatggtcgg tgcggcgttc
     5461 acacacagta cgtctgactt cggtaatatt ctgctggatg ttgcgaacaa agccattctg
     5521 cagggctggg aagatgcccc tgaaacctat gaacagtgga cgcggaaagg tcagttgtct
     5581 gattttaaaa ttgcccatcg tgtgggtatg gggggcttca gtgctctgcg tcaggtgcgt
     5641 gaaggggcag aatataaata cgtcaccacc ggagataaac aggccactat tgcactggcg
     5701 acctatggcg agctgttcag tatcacccgt caggccatta tcaatgatga tctgaatatg
     5761 ctgaccgatg tcccgatgaa gctgggccgt gcggcgaaat ccactattgc cgatctggtt
     5821 tatgccattc tgacgtctaa cccgaaaatc tccacagata atgtaagtct gttcgataaa
     5881 gcgaaacatg caaacgtact ggagagcgcg gcaatggacg tggcatcgct ggataaagcc
     5941 cgccggttga tgcgcgttca gaaagagggg gagcgtcatc tgaatattcg tcctgcgttc
     6001 gtactggtac cgacggcgat ggagtctgtt gctaaccagg tcattcgctc ctcaagtgtc
     6061 aagggggctg acattaacgc cggtattatt aacccggtga aagattttgc gaccgttatt
     6121 gcagagcctc gtcttgatga taacagccag accaccttct acctggctgc gtcaaaaggc
     6181 tccgatacga ttgaagtggc ttatctcaac ggtgtggata cgccatatat tgatcagatg
     6241 gagggcttca gtgtggatgg cgtgacaacg aaagtgcgta ttgacgccgg tgtcgcgcca
     6301 gttgatcacc gcggtctggt gaaatgtacg gcgtaaacgt cgcagacaac aactctgatg
     6361 gcccgtaagg gctttttttg tacctgaaat cagcccctga acggggctgt gcggagacag
     6421 ttatggcaaa gaattttgta gaagaaggaa aaacggtggc gattgttgcc agtgcagcca
     6481 tcagcagcgg agatctggtg caggtgggcg atgtttttgc ggtggcgctg accgatattc
     6541 cacagggtga aacaggcgac ggcatgaccg aaggtgtgtt tatgctgcct aagctgaaaa
     6601 cggatgatat gaaaacgggt aagaaggttt atctgaagtc cggaaaagtt cagctgacta
     6661 acagcggctc tgatccgctg gtcggggttg tctgggcaga tgccggaacc agtgcagaag
     6721 aagtgccggt aaaactcaat gtctgatccc ttttcccggc tggcagcgcg tatggatgct
     6781 atcacggtca gaaagatggg aaagacagcc tcgattaatg atgtcgatat gactgtgatc
     6841 ccgggagaaa cactggcaga gctgaatgct ctgtccggac ctgcggtctc tctggtggtg
     6901 ttttcttcgg gataccgccc acggcgcggg gatcgcgttg tttatgacgg acaacaatgg
     6961 acggtcacac ggcatgaacg ctttaacggt aagccaatga tctttattga gtaaagaggt
     7021 gtgggatgaa ggggcttgag aatgccatcc gcaatctgaa cagccttgat acccgtatgg
     7081 tgccacaggc cagcgcatgg gcgataaacc gtgtggcaca gaaagcggtc tcggttacca
     7141 cccggcaggt tgccgggaat accgttgcgg gagataacca ggtgaaaggg atccccctga
     7201 aactggtacg tcagcgtgtc cgggtgttta aagccagtcc gtcaggaaaa atgacggcca
     7261 ggatccgcgt taaccggggc aatctgcctg ccattaagct ggggacagcc ggggtcagac
     7321 tggcccggcg tggtggaaaa ctgcagtacc gtggcagtgt gctgaaggtg ggtaaatatc
     7381 ttttccggga tgcgtttatt cagcaactgg cgaatggtcg ttggcatgtg atgcggcgta
     7441 ttgatggcaa aaatcgttac cccattgatg tggtgaaaat cccgctgtcc ggaccgctga
     7501 cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc ggaaatgccg aaacagctgg
     7561 ggtatgcact gaaacaacaa ctgaggttat ggctgacccg atgaaccgac atacacaaat
     7621 ccgccaggtc gtactggcac gccttcggga acagtgtgga gacagcgcca cgttttttga
     7681 cgggcttccg gcatttgttg atgcgcagga actgcctgcc gtggcggtgt ggctgagtga
     7741 tgctcagtac accggaaaaa tgacggatga agatgactgg caggctgttc tgcatattgc
     7801 tgtcttcatc cgggcacagg caccggattc agagctggat atgtggatgg agagcaccat
     7861 tttcccggcc ctgaatgatg taccagcact ttccggactc atcgacaccc tgatcccact
     7921 cggttttaac tatcaacgtg ataatgagat ggccacctgg gcgatggcgg aaatcacgta
     7981 ccagatcacg tacacgaatt aaggaggtgg taatgaccac accaaatcca ctggcaaaaa
     8041 cgaaaggtgc gggaacgacg ttctggatgt ataccggcaa gggcgatgcg tttgcgaacc
     8101 ctttatcgga cactgactgg ctgcgtcttg cgatggtgaa ggatctgcaa cctggcgaaa
     8161 tgaccgctga tgcagaagat gacacttatc tcgatgatga agatgcagac tggaaaacga
     8221 caacccaggg gcagaaatcc gttggtgata cttcggcgac gctggcctgg cgtccgggtg
     8281 acagcgggca gaaaaaactg gttcagttgt tcgactccgg tgaagtctgc gcgtttcgta
     8341 tcaaatatcc caacggcact gttgatgttt tccgtggctg gctgagctca ctgggtaaaa
     8401 ccattgcctc aaaagacgtg atgacccgca ctgtgaaaat cagcggtgtg gggcgtccgt
     8461 atctggcaga ggaaggcact gaaaccgtga gcgttaccgg gctgacagta tcaccggcat
     8521 ctgccagtgt aaaagtggga gcaaccacca cgctgacctt tacagtaaaa cctgacggag
     8581 ccagtgacaa agcgatcagt gtgcattcgt cagatccaca gactgccacg gttaccctga
     8641 acggacttgt ggccacggta aaaggcgtga agcagggcag tgtcagcatt gtgggcatga
     8701 ccgctgacgg gaattttgtg gctgtggctg cggtgactgt cagcgcagca ggttaacagg
     8761 acgatactca tcatttgccc cggttattcg gggctttttt gcaggtggag aacatgatgt
     8821 ttctgaaaca ggacacgttt aattatgaaa aacagtccgt ggtgctcagt gagctgtccg
     8881 ggctgcagag aattgaatat ctgacgtttg ttcagcagcg aacggcaaag tttgatgccg
     8941 gggagggaga actgccggag gctgaacgac agattgcttt tctgcggatg gggatggata
     9001 tcaatgcctg gctggtttcc cgctcactgt ggaatgcgga acagtctcag gatgttgaga
     9061 cgctttacgc atccgttatt acaacatggt cgtatgatgc cctgggtgcg ggggcggaga
     9121 tggttctgtc gctgagcggt atgggggcca ttgataatgc cggggatttg gagcatgagg
     9181 tgctgacgcc ggaaaagtcc tgacgcggga aatgcagttt gtcatgcggc ttgcccggga
     9241 gttccggcgg gcagactggc ggcggatgct gtcggaaatg tcggccactg agcttggtga
     9301 gtggggcgat tatttccgga tgcagagctt cagtgatgtg tggatggatg cgcagtttgc
     9361 ctcgctgaag gcattgatcg tgagaatggt gtccggcagc agtgatgctg cggtggctga
     9421 tttcagcctt ttaccggaag agaacgggat accggagcga acggacgaag aactgatgca
     9481 tcttggggaa ggtatttccg gaggtgtgcg ttatggacca gatagccaac ctggtcattg
     9541 atttggggat tgatgcggca gagtttaaaa atgaaattcc ccgtatcaaa aaccttctga
     9601 atggtgcagc cagcgatgca gaacggtctt ctgcccgtat gcagcgtttt atggagcgtc
     9661 agactcaggc cgcccggcag acaacgcagg cggcgtcttc ggctgcaaca gccgcatccg
     9721 tccatgcgca gacggtggag aagaacgcac aggctcatga acgcatggcc cgcgaggtgg
     9781 agaaaacccg ccagcgtatg gaggcactga gccagaaaat gcgcgaggaa caggcgcagg
     9841 ccatggctct ggcggaggct caggataaag cagctgctgc gttttatcgt cagattgaca
     9901 gtgtgaaaca ggccagtgcg gggctgcagg aattacagcg tattcagcag cagatccgac
     9961 aggccagaaa cagtggcggg attggtcagc aggattatct ggcgctgatt tctgaggtta
    10021 cggcgaaaac ccgtgttctt acgcaggctg aggaagaggc tacccgacag aaagtggcgt
    10081 ttatccgtca gcttaaagag caggcaggaa aagccacgca ttcgctgggt ctgaaaagtg
    10141 cagcggcccg ccaggagata ggcgttctga taggtgaact ggcccgcggc aatttagggg
    10201 cgctgagggg atccgggata acgctggcta accgtgccgg atggatagac actctgatgt
    10261 caccgaaagg catgatgctt ggcggggtta ttggcggtat tgccacgtcc gtctatggtc
    10321 tgggtaaagc ctggtatgac ggtcagaagg agggggaaga atttaaccgc cagttgtcgc
    10381 tgacggggca ttatgccgga gtcactgccg ggcagctgtg gacgctcagt cgtgctattt
    10441 ccgggaatgg tatcacgcaa catgctgcag ccggtgtgct ggctcaggtg gtggggagtg
    10501 gtgcatttcg tggaaacgat atcggtatgg tggcgagagc tgccgcacag atggagcgat
    10561 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
    10621 taaatgccgc gaaggctctg gacaatgagc tgcattttct tactgccact cagcttgagc
    10681 agatacgtgt ccttggggat cagggacggt ccagtgatgc tgcacggata gccatgtctg
    10741 cactggcaga ggaaaccggt cggcgtactg cggatattga taataacctc aatgcgcttg
    10801 gcagtacgct gaggtatctg tctgatttgt ggagtcgttt ctgggatgcg gccatgaata
    10861 ttggtcgtga agactcgctg gatgaacaga ttgccgcttt acaggagaaa gtgtcgcggg
    10921 cgaaaagact cccctggacg gcatcatctt ctcaggttga atacgatcag cagcgtctta
    10981 acgatcttca ggagaaaaaa cgccagaagg atttgcagga tgcaaaagag caggcagagc
    11041 ggaattatca ggagcaacag aaacgacgta atgctgaaaa tgctgcactg aaccggatga
    11101 atgaaacgga agcagcacga catcagcgtg aaattgcgcg tattaattcc atgcagtacg
    11161 ccgatcaggc tgtcagggat gcagcgatac aacgtgaaaa tgaacgttac gagaaagccc
    11221 tggcatccgg taagaaaaaa acacgcgaaa cccgtaatga tgaggccacc cggttattgc
    11281 tgcagtacag tcagcaacag gcacaggtgg aaggacagat tgctgctgca agacagtcag
    11341 caggcattgc cactgaaaag atgacagaag cgcataaaca gcttctggct ctgcagcagc
    11401 gcatcagcga tctggacggg aaaaaactga cggcagatga aaagagtgtg ctggcccgta
    11461 aagatgaact gattcaggca ctgacgctgc tggatgtaaa acagcaggag cttcagaaac
    11521 agacggcact caacgatctg aagaaaaaaa caattcagct gaccagtcaa ctggctgaag
    11581 aagagctcgc tcagcgtcag caacatgacc tggatatcgc cacggtgggt atgggtgatc
    11641 agcagcggca gcgatatcag gtacaactga gtcttcgcca gaaataccag caacagctgg
    11701 agcagttgag gcgggatagt gagcagaaag ggacatataa cacggatgac tacagaaagg
    11761 ccgagcaggc gctgacggag agcctgaacc gacaactgaa tgagaatcgc cgttactggc
    11821 aacagcttga agttatgcag ggtaactgga aaaacggagt cctgcgtgca tttcaggatt
    11881 ttaccgtgga tgcagacaat acggcaggaa cagcagaaca ggtgttctcg tcagccttca
    11941 gcaacatggg aaatggcctg gcaacttttg tcactaccgg caaactcaac ttcaaatcct
    12001 tcacctcttc ggtgctgtca gatatggcga aaatcctggc gcaggcaacc atgatgaaat
    12061 cgataaaagg gattggcagt gtactgggat ttgatctcag cagcctttcc ctgaatgcca
    12121 atggggggat ttatcagtct gctgatttga gtcgttacag tggcacggtg gttaaccgtc
    12181 cgacgttttt tgcttttgca aaaggcgcgg gtgtgatggg ggaagctgga cctgaagcca
    12241 ttctgccact gcgtcgtggt gctgacggta agctgggggt tgtggcggat attggtggtt
    12301 caggtatggc gatgtttgcc ccgcagtaca acatcgagat caataacgat ggcacgaacg
    12361 ggcagatagg tccggctgcc ctgaaggtgg tttatgacct tgggaaaaaa gcggcagcgg
    12421 actttatgca acagcaggcc cgtgatggtg gtcggttaag tggagcatat cggtaatgga
    12481 gacgtttcac tggaaagtgc gcccggatat gaatgtggta tcagagccga aagtggtgac
    12541 agtgaagctg ggcgatggtt atgaacagcg tcgtgcggcg ggactgaata accagttgtc
    12601 gacttacagc gtgacgatac gtgttcgtaa atgtgaacac ccatctttaa aagcctttct
    12661 ggaacggcac ggtggcgtcc gcgcatttca gtggacgcca ccttatgact ggaagccgat
    12721 cagggtggtt tgtcgtaaat ggtcggcaag cgtgggggcg ttgtgggtaa ccataacggc
    12781 agattttgaa caggtcgtgg cataggagtc tctgatgcag gatattccac aggaaacaca
    12841 tcatgagacg acacgcctca ctcagtcagc ccaggtggtg ctctgggaaa tcgatctgac
    12901 agtggtcggt ggagaacgtt attttttctg taatgagcag aacgaaaaag gtgagccggt
    12961 cacctggcag gggcgacagt atcaggcgta tctcattcag gggacgggat ttgaactgaa
    13021 cggcaagggc agtgctgccc gtccgacact gacggtttct aacctgcacg gcatggtcac
    13081 cgggatggcg gaagacctgc agagtctggt cggaggaacg gtggtccggc gtaaggttta
    13141 cgcccgtttt ctggatgcgg tgaacttcgt caacggaaac agtgacgccg atccggagca
    13201 ggaggtgatc agccgctggc gcatcgagca gtgcagcgaa ctgagcgcgg tgagtgcctc
    13261 ttttgtactg tccacgccga cggaaacgga cggtgccgtt tttccgggac gtatcatgct
    13321 ggccaacacc tgcacctgga cctatcgcgg tgatgagtgc ggttatcacg gtccggcggt
    13381 cgcggatgaa tatgatcagc cgacgtccga tatcacgaag gataaatgca gcaaatgcct
    13441 gagcggctgt aagtttcgca ataacgtcgg caactttggc ggcttccttt ccattaacaa
    13501 actttcgcag tgaatcccat gaca
//
LOCUS       DADSZD010000120        13504 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.121, whole genome shotgun sequence.
ACCESSION   DADSZD010000120 DADSZD010000000
VERSION     DADSZD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.121"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..507
                     /locus_tag="J1Z28_003210"
     CDS             <1..507
                     /locus_tag="J1Z28_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBA7718020.1"
                     /translation="KLVAEKVAQGAHADLISIDNEGNITEQEWEKLDAADAIIFGAPT
                     YMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDKQVTLIYLQTLASQHG
                     GLWVSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQIPTGDLDTAVKYGERV
                     ATITVRLK"
     gene            complement(678..1670)
                     /gene="rpoS"
                     /locus_tag="J1Z28_003211"
     CDS             complement(678..1670)
                     /gene="rpoS"
                     /locus_tag="J1Z28_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBA7718021.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(1733..2872)
                     /gene="nlpD"
                     /locus_tag="J1Z28_003212"
     CDS             complement(1733..2872)
                     /gene="nlpD"
                     /locus_tag="J1Z28_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBA7718022.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(3012..3638)
                     /gene="pcm"
                     /locus_tag="J1Z28_003213"
     CDS             complement(3012..3638)
                     /gene="pcm"
                     /locus_tag="J1Z28_003213"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBA7718023.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(3632..4393)
                     /gene="surE"
                     /locus_tag="J1Z28_003214"
     CDS             complement(3632..4393)
                     /gene="surE"
                     /locus_tag="J1Z28_003214"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBA7718024.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(4374..5423)
                     /gene="truD"
                     /locus_tag="J1Z28_003215"
     CDS             complement(4374..5423)
                     /gene="truD"
                     /locus_tag="J1Z28_003215"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBA7718025.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAVRSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(5420..5899)
                     /gene="ispF"
                     /locus_tag="J1Z28_003216"
     CDS             complement(5420..5899)
                     /gene="ispF"
                     /locus_tag="J1Z28_003216"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBA7718026.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(5899..6609)
                     /gene="ispD"
                     /locus_tag="J1Z28_003217"
     CDS             complement(5899..6609)
                     /gene="ispD"
                     /locus_tag="J1Z28_003217"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBA7718027.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(6628..6939)
                     /gene="ftsB"
                     /locus_tag="J1Z28_003218"
     CDS             complement(6628..6939)
                     /gene="ftsB"
                     /locus_tag="J1Z28_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBA7718028.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(7132..7455)
                     /locus_tag="J1Z28_003219"
     CDS             complement(7132..7455)
                     /locus_tag="J1Z28_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBA7718029.1"
                     /translation="MRNSHNITLTNNDGLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(7505..8110)
                     /gene="cysC"
                     /locus_tag="J1Z28_003220"
     CDS             complement(7505..8110)
                     /gene="cysC"
                     /locus_tag="J1Z28_003220"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBA7718030.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(8110..9537)
                     /gene="cysN"
                     /locus_tag="J1Z28_003221"
     CDS             complement(8110..9537)
                     /gene="cysN"
                     /locus_tag="J1Z28_003221"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBA7718031.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(9539..10447)
                     /gene="cysD"
                     /locus_tag="J1Z28_003222"
     CDS             complement(9539..10447)
                     /gene="cysD"
                     /locus_tag="J1Z28_003222"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBA7718032.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            10699..11736
                     /gene="iap"
                     /locus_tag="J1Z28_003223"
     CDS             10699..11736
                     /gene="iap"
                     /locus_tag="J1Z28_003223"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBA7718033.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDVRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEVFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTATFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(11784..>13504)
                     /gene="cas3"
                     /locus_tag="J1Z28_003224"
     CDS             complement(11784..>13504)
                     /gene="cas3"
                     /locus_tag="J1Z28_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBA7718034.1"
                     /translation="LSLLKDKNIVTFFDFWGKNRRGEKDGGDDYHLLCWHSLDVAAMG
                     YLMVKSNCFGLADYFRQLGFADTEQAAQFFAWLLCWHDTGKFARSFQQLYLHPQLKVP
                     EGARKNYEKISHSTLGYWLWHHYLIECEELLPSSSLSLRKLKRVIDMWMPVTTGHHGR
                     PPDRIDELDNFLPEDKAAARDFLLEIKVLFPLIEIPTFWDDDEGVELIKQLSWYISAT
                     VVLADWTGSSTRFFPRVAQTMDIEDYWQKTLVQAQNALTVFPPKAETAPFTGINTLFP
                     FIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLMAAGKAQGLFFG
                     LPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNKSLWSGDLVGSEEP
                     DEQTFSQGCAAWVADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLAD
                     EIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGIEGQQEAPF
                     LEKDDYPWLTHVTKSDVHSHRVATRKDVERSVSVGWLHSEQECIVFPAPAGINRSRFW
                     GTVTHN"
BASE COUNT         3230 a         3665 c         3344 g         3265 t
ORIGIN      
        1 aaactggttg ctgaaaaagt ggcacaaggc gcccatgcag atttaatttc gattgataac
       61 gaaggaaata tcactgagca ggagtgggaa aaactggacg cagccgatgc cattatcttc
      121 ggtgctccca cctatatggg cggtgcgcca tggcaattca agaaatttgc tgatgccagt
      181 tccaaagcgt ggtttacacg taaatggcag gataaagtct tcgggggttt caccaacagc
      241 gccagcctga atggcgataa acaagttacg ctgatctatc tgcaaacgct ggcttcacag
      301 catggcggtc tgtgggtgag tttaggtcag gctcctgcca atgtgcttgc gtcaaccagg
      361 gaagatgtaa acaatctggg cggttcagtc ggtttgctgg tgcaatctcc agccgatgcg
      421 ggtgcagacc aaatccctac gggcgatctg gatacagccg tgaaatatgg tgaacgcgtg
      481 gcgacaatta ctgtgcgctt aaaatgatga ttgccaaatg tgacgctgac gcgtctatcc
      541 ggtcttctac atctaaaaag tgccgtcccc tggtcagtca gataaagttt taacgctgta
      601 tccgataaca gttcaacatg taccaaaaga aaaaaggcca gcctcgcttg agactggcct
      661 ttctgacaga tgcttactta ctcgcggaac agcgcttcga tattcagccc ctgcgtttgc
      721 aggatttcgc gcaaacggcg caggccttca acctgaatct ggcgaacacg ttcacgggtg
      781 aggccaattt cacgacctac atcttccagt gttgccgctt cgtaccccag caaaccgaat
      841 cgacgtgcca gcacttcacg ctgtttggcg ttcagctcga acagccattt gacgatgctc
      901 tgcttcatat cgtcatcttg cgtggtatct tccggaccat tctctttttc atcggccagg
      961 atgtccagca acgctttttc ggaatcacca cccagcgggg tgtctaccga ggtaatgcgc
     1021 tcgttaagac gaagcatacg gctgacgtca tcaactggct tatccagttg ctctgcgatc
     1081 tcttccgcac ttggttcgtg gtccagctta tgggacaact cacgtgcggt tcgcaggtaa
     1141 acgttcagct cctttacgat gtgaatcggc aaacgaatag tacgggtttg gttcataatc
     1201 gcccgttcga tcgtctggcg aatccaccag gttgcgtatg ttgagaagcg gaaaccacgt
     1261 tccgggtcaa acttctctac cgcgcggatc agccccaggt tgccctcttc gatcaggtcc
     1321 agcaacgcca gaccacgatt gccataacgg cgggcaattt ttaccaccag acgcaagtta
     1381 ctctcgatca tccggcggcg agaggcgaca tctccacgca gtgcgcgacg cgcaaaataa
     1441 acttcttctt cggccgttaa cagtggtgaa taaccaatct caccaaggta aagctgagtc
     1501 gcgtccagca cacgctgtgt ggctccctgc gataacagtt cctcttcggc caaatcgtta
     1561 tcactgggtt cctcttctac taaggccttt tcgtcaaaaa cctcaactcc gttctcatca
     1621 aattccgcat cttcatttaa atcatgaact ttcagcgtat tctgactcat aaggtggctc
     1681 ctacccgtga tcccttgacg gaacattcaa gcaaaagcct ggttccgccg atttatcgct
     1741 gcggcaaata acgcagcggg tttacggatt tccccttgta acgaatttca aaatgcaagc
     1801 gtgttgaact ggttccggtg ctacccatgg ttgctatttt ttgccccgcc ttcacttctt
     1861 gttgttcccg gaccagcatt gtgtcgttat gggcgtaggc actcaggtaa tcatcattat
     1921 gtttgatgat aatcagatta ccgtagccgc gcagcgcgtt accggcataa acaacgcggc
     1981 catctgcggt cgcgataatt gcctgtcctt tgctgcctgc gatatcaatc cccttgttgc
     2041 ccccctcaga agcgccaaag gtttcgatca ctttgccctc agtcggccag cgccaggtgg
     2101 agataggcgt actggttgat gtactgctga cagtcggctc ggttgtgctt gctgttggca
     2161 ccgttacagg cgctgtgacc gtggtcgcag ttggcttgtt gttcggcaac attttgttag
     2221 cactctgttc acccgaagac tcagaatacg taattgtcgg ttgcgacgca acagcaacgg
     2281 tggaattttg tgcaggcttg atcacaactc cttgctctgc tgcgtcggcc tgggtaatgg
     2341 catttccgcc tgtgattggc gtaccggaag cattacccac ctgtaaggtc tgaccgacgt
     2401 tcagcgcgta tggtgcctga atattgttgc gctgagcaag gtcacggaaa tcgttgccag
     2461 taatccaggc gatatagaaa agcgtgtcgc cttttttcac ggtgtatgta ctgccgctat
     2521 aactgccttt cggaatgttc ccatactgac ggttgtagac gatgcgtccg ttttccatct
     2581 gtaccggctg ctgagctact ggctgcacag gctggatttg cggttgttga gtagcctgaa
     2641 tttgtggctg ctgtaccggc tgaatttgcg gttgctgcgc tgtagacgtc gtccccattt
     2701 tcggcggcgg cgtaatcaac ataccagaat tggtatttgc aggcgcattg ccattaacgg
     2761 agctgaccgg tgccggtgga tttgaagtgt cagaacagcc tgccagccat agcgaaacca
     2821 gtgacaatgc cgcaatgcgg cgaacggtga attttgggct tcccgcgctc atttatcccc
     2881 caggaaaaat tggttaataa ccagtgacat aattaccgtg caaggcacca tactgaacac
     2941 tggaaaagat gttcacgata cgctgacttg cggcaaaata accaggaaaa atccaggtat
     3001 ttcctcacgt tttaagccag ctcacccttc actaaaggga caaagcgcac ggcctccacg
     3061 gtatcgataa taaattcgcc tccccgacga cgcacccgtt tcaaatactg gtgctcctcc
     3121 cctacgggta agacgagaat cccgccttcg tccagctgcg tcattagcgc agttggaatt
     3181 tcaggcggtg ccgccgtaac aatgatagcg tcaaacggcg cgcgtgcctg ccaaccttgc
     3241 catccatcgc catgacgggt tgaaacatta tgtaaatcaa gatttttcag gcggcgacgc
     3301 gcctgccact gcaagccttt aatccgttca accgagcaaa catgctggac aagatgcgcc
     3361 aggattgcag tttgatatcc cgaaccagtg ccaatttcca gcacccgcga ctgcggcgtc
     3421 agctcgagta attcggtcat tcgcgccacc atatacggct gcgaaattgt ctgcccctga
     3481 cctatcggca aggcgatatt gtcccaggct ttttgttcaa acgcttcatc aacgaatttt
     3541 tcacgcggca cggcggcaag tgcattcagc acctgctcat cctgaatacc ttgcgcacgt
     3601 aattgatcca gaagtgcttg tacgcgtctg cttaccattg cgtgccaact cccacgctgt
     3661 ttaaccagtc tgaaaccaca tcttgcgcgc tatgcgcagt taaatccaca tgcagcggcg
     3721 tgatggaaac atagccctca tctaccgcag caaaatcggt ccccggaccg gcatcacatt
     3781 taccgcccgg cgggccaatc cagtacagcg tattgccgcg cggatcttgc tgcgggatca
     3841 cctgatctgc cggatgtcgt gtaccgcagc gcgtcacgcg aatacctttg atttgatcca
     3901 atggtaaatc cggaacgtta atattaagaa tacgcccggt gcgcagcggc tctttacaca
     3961 gtgcgcgcaa aattgagcag gttaccgccg cggcagtgtc gtaatgttta tgcccgtcaa
     4021 gcgagacggc aagcgccgga aaacctaaat ggcggccttc catcgcggcc gctaccgtac
     4081 cggaataaat aacatcatcc cccagattcg gcccggcgtt aattccggac acaacaatgt
     4141 ccgggcgcgg gcgcatcaga gcatttacgc caagatagac gcaatcggtc ggggttccca
     4201 tttgcacagc aatatcacca ttttcaaagg taaacgtgcg cagggaggat tccagtgtca
     4261 gagaattaga agcgccgctg cggttacgat cgggggcgac cacctgaacg tcagcaaact
     4321 cacgcaaggc tttcgccagc gtttgtatac cgggtgcatg taccccgtca tcattactca
     4381 gcaatatgcg cataatcacc tgttgtgttg ataagttccc tgacaacgct ggttgcaaaa
     4441 cttcccgccg gaagccagaa acggatttct acggtgacgt catcccacca gttccagctt
     4501 aattgttgcg gatacagcag catcgctctg cgcgcggctt caactttttc acgcaccagt
     4561 aaagtttgta attcagtttc ttcggcgaca gctgcttgtt cgaatgccag cgcttcacgc
     4621 tgagttcccc attcgccact gcccggcaat gcggcggtta tcatcagctc tttatcgttg
     4681 acgcgacgct gtaattccgc cagttcttcg gtggttgcaa caaaccagct accacgtccg
     4741 gctaattgta gcgcatcgcc gtcaacaact tgattaacgt ctgctttttt gaggcgctca
     4801 gcaacaatct gattaaacaa cgcactgcgg actgccgaca accaaaaact ccgtttattg
     4861 cgatcgcgca ccggagtatt ggtttgcgcc cagcgcagcg caccctgcaa gttgctaccg
     4921 ccaatcccaa aacgttgggc accgaagtag ttcggtacac cttttacgca aatatcgatc
     4981 agacgttgtt caacgtcatc gcgattgctc acttcgcgta gtaccagggt aaaggcgtta
     5041 cctttcagcg cccctaaacg cagcttgcgc ttgtgccgcg catactccag cacctggcag
     5101 ccttccagtt gaaaggcgct cagatcgggc atctctttgc ccggcacgcg agcgcataac
     5161 cactgttccg ttacagcatg tttgtctttt tgcccagcga agctgacttc acgggcatga
     5221 attttcagga atttcgccag tgcatccgcc acaaaacggg tattgcaacc gtttttgagg
     5281 attctaacca gaatatgctc accttcacca tcaggctcaa agcccaaatc ttccaccacc
     5341 acaaagtctt ccggattcgc tttcagcagc ccggtgcctt gcggtttacc gtggaggtaa
     5401 gtgagattat caaactcaat cattttgttg ccttaatgag tagcgccacc gcttcacagg
     5461 caatcccttc cccacgtccg gtaaatccca gtttttccgt agtagtggct ttcacgttaa
     5521 catcatccat atggcagccg agatcttcgg caataaacac gcgcatttgt ggaatgtgcg
     5581 gcaacatctt cggtgcctga gcgatgatag tgacatcgac gttgccgagg gcataacctt
     5641 tcgcctgaat acgacgccag gcttcgcgca acagctcgcg gctgtcggca cctttaaatg
     5701 ccggatcggt atccgggaac agcttgccta tatctcccag cgccgccgcg ccaagcaatg
     5761 catcggtcaa cgcatggagc gccacgtcgc catcagaatg cgccagcagc cctttttcgt
     5821 aaggaatgcg tacgccacca atgataattg ggccttcacc gccaaaggcg tgtacgtcaa
     5881 aaccgtgtcc aattcgcatt atgtattctc ctgatggatg gttcgggtga ggtaaaattc
     5941 ggccagcgcc aaatcctccg ggcgcgttac cttgatgtta tccgcgcggc cttcaacaag
     6001 ttgcggatga aaaccgcaat attccagcgc cgaggcctca tcggtaatgg tagcgccttc
     6061 atttagagcg cgcgtcaggc agtcatgtaa cagctcacga gggaaaaatt gcggcgtcag
     6121 cgcgtgccat aagccgttgc gatcaacggt atgagcaatg gcatttttgc ccggttcggc
     6181 acgtttcatc gtatcgcgca ccggcgcagc gaggatccct cccgtgcggc tggtttcgct
     6241 caacgccaac aatcgcgcaa ggtcatcctg atgcagacaa ggacgagcgg cgtcatgcac
     6301 caatacccac tgcgcgtcgc cagcggcttt cagacctgcc agcacggaat cggcacgctc
     6361 ttcaccacca tcaacaaccg tgattcgtgg atgattcgcc agaggaagtt gtgcaaagcg
     6421 gctatcgcca ggacttatgg caatgacgac acgtttcacc ctgggatgcg ccagcagcgc
     6481 atgcaccgag tgttcaagaa tggtttgatt accgattgag agatattgct taggacattc
     6541 cgtttgcatt cggcggccaa atccggccgc cggaaccacg gcgcaaacat ccaaatgagt
     6601 ggttgccatg ttaattcccg ggctgattta tcgattgttt tgccccgcag actgtgcgcg
     6661 ctttgacgcg tcaggcacca gacgataaaa cgtttcgccc ggcctggtca tgctgagttc
     6721 attacgcgca cgctcttcga gcgcctcctg gccgccattg agatcgtcaa tttcggcaaa
     6781 aagctgatcg tttcgcgctt taagtttcgc gtttgtagct tgctgtgccg ccacatcatt
     6841 attgacgcga gtatagtcat gtataccgtt cttaccgaac cacagcgaat actgtagcca
     6901 gaccagaata gccagcaaca gcagcgttag tttacccatc ctgccccctg aaaaacggca
     6961 tcttcatccc atgcatccga agacgactct acatcctctg ttggggatac cgcgacaacg
     7021 cgggcaaatg taccacattt gtccattgtt acgtataccc agggcgtgca gaacataatc
     7081 tcattattag ttacggattg aattatgaac agaatagaga ggaaagaaca attaacccag
     7141 tagccacata aacagcgcac caaacataat gccaaccgtc gccagagtga aaacaatact
     7201 gtagcgtagc tttccgtcca tcaatgaatg cagtgcaatc cccaccacca ctgcgacggg
     7261 catcagcgcc agaaagaaag gccaggtgta gataaaaaag agcagcgtgt tagagccata
     7321 aatcaacatc ggaattgcca acgcgcacag ccacgataaa aaaccaacca cagcgcctgg
     7381 cagcgaccat gtggtgtctt caacctcagt aaggccgtcg ttatttgtta atgtaatgtt
     7441 gtggctgtta cgcatatctg atcctgttac tttgacgaac cgggcatgaa aacccggtgg
     7501 tgtctcagga tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat
     7561 ttgttactaa ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg
     7621 aatctattcc cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aaaccttttg
     7681 gatcgcgggc ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt
     7741 ctcctacgcg ttcgcgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca
     7801 gcaccaccag tccggcttca accatcaaat tcgccacttc accgacgcga cggatattct
     7861 ctttacggtc ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acgttgtcgc
     7921 catccagcag atacgtactg acgccaagtt tatgtaacgc ctcctccagc gcccccgcga
     7981 cggttgattt accggaacca gagaggccgg taaaccacag cacaacacca cgatgaccgt
     8041 ggtgctgttc gcgttgttgc ggagttactg ggtggctatg ccagacgacg ttttcgtcat
     8101 gcagcgccat tatttatctc ccagtaaatc gcgcgcaccc caatgcggga aatggcggcg
     8161 aaccagggca ttcaattcca gttcgaatgc actgaattca gatggcgcag cagttgcctg
     8221 gctaactggt tcgtgcacca taccggcacc tacggtcaca ttactcaggc gatcgataaa
     8281 aatcagccca ccggtaaccg ggttttgctg ataacgatct aacaccagtg gctcgtcaaa
     8341 ggtgagatct accaggccga tgccgttcag cggcaggttt tcaacttcgc gttgggtaag
     8401 gttattgata tcaacctgat aacgaatgcc atcaacacga gcacgcgtct tcttaccggc
     8461 aattttgatg tcgtaactct ggcccgggga aagcggctgt tccgccatcc ataccacatc
     8521 caccgacgcg ctctgcacag ccggtaacgc ttcgtctgcc gccagcagca gatcgccacg
     8581 gctgatgtcg atctcatccg tcagcaccag ggtgatcgct tctccggcaa aggcttcttc
     8641 gcgatcgcca tcaaaggtca cgatccgcgc gacgtttgat tccacaccag agggcagcac
     8701 tttgacacgt tgcccgactt ccacgcgacc ggatgccagc gttccggcgt agccacgaaa
     8761 atcgagattc ggacggttaa cgtactgcac cgggaagcgc attggctgag catccaccac
     8821 tcgctggatc tccacggttt ccagcacttc tagcagtgtc ggaccgctgt accacggcat
     8881 actttcactt tgcgaagcca cgttgtcgcc ttccagtgcg gagagcggca caaagcggat
     8941 atccagatta cccggcagct gcccggcaaa agtcagataa tcttcacgaa tacgggtgaa
     9001 cgtctcttca ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat
     9061 ccccaacagt gtggagataa aactatgacg acgggtttga tcgagcacgc ctttacgggc
     9121 atcgatcagt aagatcgcca gttcacatgt cgatgcgcca gtcgccatat tgcgggtgta
     9181 ctgctcgtgc cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaatagcg
     9241 gtaggccaca tcaatagtga tgccctgttc gcgctcagct tgcaggccgt ccaccagcag
     9301 tgccagatcc agcttttcgc cctgggtgcc gtgacgctta ctgtcgttat gcagcgatga
     9361 gagctgatct tcgtagattt ggcgggtatc gtgcagcagg cgaccaatca gggtactttt
     9421 gccgtcatcg acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat
     9481 catccatgct tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt
     9541 aaaaataccc ctgacgtttt ttcagctcca tagaccccgc ctggtcgcgg tcaattacgc
     9601 ggccctgacg ttcactggtg gtggaaacca gcatctcttc gatgatctcc ggcagtgttt
     9661 gcgcatttga ctccaccgcg ccggtcagcg gccaacagcc cagtgtgcgg aaacgcacca
     9721 tccgtttttt aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca
     9781 acataccgtc gcgttccaga accggacgtt ccgcagcgag atacagcgga acaatgtcga
     9841 tattttccag ccagatgtat tgccagatat cctgctcggt ccagtttgag agcgggaaga
     9901 cgcggatgct ttcgcctttg ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct
     9961 gatttttcgg atcccagcga tggaagcggt cacggaaaga gtagattcgc tctttagcgc
    10021 gggatttctc ttcgtcacgg cgcgcaccac cgaaggcggc atcaaaaccg tatttgttca
    10081 gcgcctgttt caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgtacga
    10141 atggattaat ccccatcgcc acgccttccg ggtttttatg caccagcagc tcgcagccgt
    10201 aggctttcgc cgtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcga
    10261 catgcagcaa cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca
    10321 tgacgctgga atctttaccg atagagtaga gcatcaccgg atttgagaat tctgcagcca
    10381 cctcgcgaat aatgtggatg ctttccgcct ccagttgccg caggtgggta agtcgttttt
    10441 gatccataac cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata
    10501 gggagcgtaa gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa
    10561 cgatttggca aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa
    10621 gatttgaaat tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat
    10681 ggtatttaag gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc
    10741 ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat
    10801 acccaggcac gacatattgc tactttcttt ccgggacgca tgaccggaac tcctgcagaa
    10861 atgttatctg ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatgtt
    10921 cggacattta atagtcggta tatttatacc gcccgcgata atcgcaagag ctggcataac
    10981 gtgacgggaa gtacggtgat tgccgctcat gaaggcaaag cgccgcagca gatcatcatt
    11041 atggcgcatc tggatactta cgccccgctg agcgatgctg acgccgatgc caatctcggc
    11101 gggctaacgt tacaaggaat ggatgataac gccgcaggtt taggtgtcat gctggaactg
    11161 gctgaacgcc tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc
    11221 gaagaggaag gaaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag
    11281 aaaaatacgc tgctggtgat taatctcgat aacttaattg ttggcgataa gctgtatttc
    11341 aacagtggcg taaaaacacc tgaagcagta aggaaattaa cgcgcgaccg ggcgctggca
    11401 attgcgcgta gtcatggaat tgccgcaacg accaatccgg gtttgaataa aaattatccg
    11461 aagggtactg gctgttgtaa tgacgccgaa gttttcgaca aagccggtat tgccgtactt
    11521 tcggtagaag caacaaattg gaatcttggg aataaggatg gttatcagca acgcgcaaaa
    11581 acagccacat tccctgcggg aaatagctgg catgacgtaa gactggataa tcaacaacat
    11641 attgataagg cacttcctgg aagaatagaa cgtcgctgcc gtgatgttat gcggataatg
    11701 ctaccgctgg tgaaggaact ggcgaaggcg tcatgaggcg tttgaaaatg ggagctggga
    11761 gttccccgct gacgcggggg agctcagtta tgggtaacag taccccagaa ccgagagcgg
    11821 tttatccccg ctggcgcggg gaacacaata cactcttgtt cgctatgaag ccaacccaca
    11881 ctcacgctgc gctcaacgtc tttgcgcgtc gctacccggt gtgaatgcac atcggatttc
    11941 gtaacgtgcg tcagccaggg gtaatcatcc ttttccagga acggagcttc ttgctggccc
    12001 tctataccac gcgcaaaggc agcaacgagt ttgtcgcgct gctgttgaga taacgtggca
    12061 gaaagcaaaa tgacgctgtt tccgccacgc gcctgccgct cgatcagccc ttcaagaatg
    12121 catgacatgt aagcgtcaca ggcatggatc tcatcagcca gcaggatttt gttactcagt
    12181 cccagaagcc gcagattatt atgtttgaac ggcatcacag ccatcatcgc ctgatccagc
    12241 gtgccgacgc caatttcagc cagtagcgcc ttcttgttac tgtcggcaac ccaggccgca
    12301 catccctgac tgaacgtttg ttcatccggt tcttctgacc cgactaaatc accggaccag
    12361 agtgatttat taaagcggtc cattaatgtt cgggcactgt gtgccagcac caggctgggg
    12421 cgggactctg gcgaatagaa agcaagccag gttttgacca gtcgatcgta catggcattg
    12481 gccgttgcca ttgttggcag gccaaaaaat aaaccctgcg ctttccccgc agccatcaac
    12541 ctgtgcgcca ggataagcgc cgcttctgtt ttacctgcgc cggttacatc ctccagaata
    12601 aataactgtg gtcctggttg gctgatatcc agatccagca ccttttgctg taacggtgtt
    12661 gggttctcaa taaaaggaaa cagcgtatta attccggtga aaggcgcggt ttctgctttt
    12721 ggaggaaaga cggttaaggc gttttgagcc tgaactaaag ttttctgcca gtaatcttca
    12781 atatccattg tctgtgcgac gcgtggaaaa aatcgcgttg acgatcccgt ccagtctgca
    12841 agtacgacgg ttgcagagat ataccaggaa agttgtttta taagttcaac gccctcgtca
    12901 tcatcccaga atgtggggat ctctatgagc ggaaacagta ccttgatttc aaggagaaaa
    12961 tctcgtgcgg cagctttgtc ttcaggcaga aagttgtcca gttcatcgat acggtcagga
    13021 ggccgaccat gatgcccggt cgtcacgggc atccacatat ctattacccg tttaagtttg
    13081 cgcaaagaga gtgaagatga aggaagcaac tcttcacatt caattaaata atgatgccac
    13141 aaccagtaac ccagcgttga atgagagatc ttttcgtaat tctttcttgc gccttccgga
    13201 accttgagtt gtgggtgcag gtaaagttgc tgaaaagagc gggcaaattt tccagtatcg
    13261 tgccagcaca gcaaccaggc gaaaaattgt gctgcctgct ccgtatcagc gaaacctagt
    13321 tgacgaaaat agtcagccag cccgaagcaa ttgcttttaa ccattaaata gcccatcgcg
    13381 gctacatcca gcgaatgcca gcaaagaagg tgatagtcgt cgcccccgtc tttctcgccg
    13441 cgtcggtttt ttccccagaa atcaaaaaaa gtcacaatat ttttatcctt cagtaaactt
    13501 aaag
//
LOCUS       DADSZD010000121        13223 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.122, whole genome shotgun sequence.
ACCESSION   DADSZD010000121 DADSZD010000000
VERSION     DADSZD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.122"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..378
                     /locus_tag="J1Z28_003225"
     CDS             <1..378
                     /locus_tag="J1Z28_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA7718035.1"
                     /translation="ACRPTEELVKRMLNKGLESLAEGEKPLLHSDQGWHYRIKSYQAA
                     LADKGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDYRSVEELENAVNEYITYWNQ
                     KRIKLSLGGLSPVEYRTEYQKAG"
     gene            complement(453..779)
                     /locus_tag="J1Z28_003226"
     CDS             complement(453..779)
                     /locus_tag="J1Z28_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBA7718036.1"
                     /translation="MFRSLFLAAALMVFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(894..2150)
                     /locus_tag="J1Z28_003227"
     CDS             complement(894..2150)
                     /locus_tag="J1Z28_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBA7718037.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPIPPSALPG
                     LIVALILGLAGGVSLGPEHPIITVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     QMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVMGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            2354..3319
                     /gene="glk"
                     /locus_tag="J1Z28_003228"
     CDS             2354..3319
                     /gene="glk"
                     /locus_tag="J1Z28_003228"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBA7718038.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            3538..3864
                     /locus_tag="J1Z28_003229"
     CDS             3538..3864
                     /locus_tag="J1Z28_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBA7718039.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3886..5133
                     /locus_tag="J1Z28_003230"
     CDS             3886..5133
                     /locus_tag="J1Z28_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBA7718040.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            5149..6234
                     /gene="ypdF"
                     /locus_tag="J1Z28_003231"
     CDS             5149..6234
                     /gene="ypdF"
                     /locus_tag="J1Z28_003231"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA7718041.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQFELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEYIRRFIQAGMSE
                     CEIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISVIRPGVRCQQVDDAARRV
                     ITEAGYGKFFGHNTGHAIGIEVHEEPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            6234..7271
                     /gene="ypdE"
                     /locus_tag="J1Z28_003232"
     CDS             6234..7271
                     /gene="ypdE"
                     /locus_tag="J1Z28_003232"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBA7718042.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            7296..9791
                     /gene="ptsP"
                     /locus_tag="J1Z28_003233"
     CDS             7296..9791
                     /gene="ptsP"
                     /locus_tag="J1Z28_003233"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBA7718043.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGELQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9794..10651)
                     /locus_tag="J1Z28_003234"
     CDS             complement(9794..10651)
                     /locus_tag="J1Z28_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7718044.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWIQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRHQFGLTP
                     GEYSARFQG"
     gene            complement(10664..11401)
                     /locus_tag="J1Z28_003235"
     CDS             complement(10664..11401)
                     /locus_tag="J1Z28_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBA7718045.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTTPAATVTRENDTINLVKDERIIVTPINDIYYAE
                     AHEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTY
                     ILRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11416..13113)
                     /locus_tag="J1Z28_003236"
     CDS             complement(11416..13113)
                     /locus_tag="J1Z28_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBA7718046.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3158 a         3350 c         3601 g         3114 t
ORIGIN      
        1 gcctgcaggc ctacagagga gctggtgaag cggatgttaa ataaggggct ggaaagcctt
       61 gcagaaggag aaaaaccgct gctccacagt gatcaagggt ggcattaccg gataaaaagt
      121 tatcaggctg ccctggcaga caaagggtta gtgcagagca tgtcgcgcaa gggcaactgt
      181 cttgataatg cggtaatgga gaatttcttt ggtcacctga aagaagaaat gtactatcgc
      241 cgggactaca gaagtgtaga agagctggaa aatgccgtta acgaatacat aacttactgg
      301 aatcaaaaaa gaataaaact cagtcttggg ggactcagcc cggtagaata ccggactgag
      361 tatcaaaaag ccggttaact gaaactgtcc aggttttggg gttcagttca gctagcgtcg
      421 catcaggcat tgtgctccat tgacggatgc gtttaatggc ggtgtccatg tccgcggcct
      481 tttccacgat gatcgtcacg atcgcgccag ccttcacgat agccacgctc atacgcttta
      541 cgcttatccc agccacgatg gtagccatta tcatgacgcc accagcggtt tttacgccat
      601 tcataattgc ggtgccagta gtcacggtcg cgccagtgac caccgtccca gtaattaccg
      661 taatgatcgc gatcgccaat ttgtaatttg attgatggca gtagggtgat ttcacctgcg
      721 tttgccgcaa gcggggtaaa taccatcagg gcggccgcca gaaacagtga cctgaacatt
      781 gttattctcc ttcacgatcg aagccgtcag cggcctgtta acgcaatatt acggggaggt
      841 aaagcgccgt atcatcgcca taactcttaa atcataaagg agagcatttt ttgctaaacg
      901 ttatcgtggg gtggctgttg cttcggacga ttgaccatca tcatcggttt acctgctaat
      961 aacagccatg ccggaagcat gacgatacag agcagcggta gcaatgtggt attgggtaca
     1021 acgactgccg ccataaaaag acttaaccag ccatcgcgtg ttaccaccag aacgatgccg
     1081 agaatagcgc aggaaacggt tattgccgct ggaacggcgg ggacgtgctc atgcagcatc
     1141 aaccctaatg ccacgccgac aaacactgcc gggaagattc gcccaccgcg aaagccactg
     1201 gcggcagcaa cgaccagggc ggcaagttta atcaccgcca gcaaaaagta atcgctggtg
     1261 ctgaaagcct gatttgccac catctgctgc atctcatcca gccctttaaa cagcgaaact
     1321 ggtccaccca taacccccag aataccgaga ataaatccgc caatacccag cacgagcacc
     1381 ggatttttca tttgatgcat catcgcgtgc aatcgtggta agcaccatac ggcgaccatc
     1441 cctgctgcga tggcaatcgc cgcgacaatt gcaccactga gaatatcggt catttgcatc
     1501 tgtccgtaat gcgcaatggg cagtgaaaaa tgaggatgga aaaataatcc ggtagttagt
     1561 gcaccagctg ctgccgccat taacggcgca aagagacgat cccatagcgg aacttcacta
     1621 ctgccattta aggtttgtga aaatatcaac gccgccgcaa caggtgtgcc aaacagcgcg
     1681 ccgatggttc cggcagaggc taaaatagtc cactccattc ggttgacgcg cggtaacaga
     1741 cgagcgccaa tggcgacggc aagggcaata ttgacggtta tgatcggatg ttccggcccc
     1801 aggctgacgc cgccagcaag accgagaatt aatgctacga taagtcctgg tagcgcagag
     1861 ggcggaatcg gtgcgccgat aagcggttca caggcgggat ctggtccggc atgaccctgg
     1921 ctgaaacgga taaccaaccc caccgcaata cccgtcagcg ttaatacacc gatgatccag
     1981 agaggtgaat cctgggctat ccccagagtt cccggcagtc gttgccagag caaattctgt
     2041 aataccgagg cgattttcat caccataatc agaataagac tggacgcaat cccaattgcc
     2101 acggcgggga gcgataataa caacatggtt ctggctcgcg gatggagcat gttctattcc
     2161 ttatgcgggg tcagatactt agtttgccca gcttgcaaaa aggcatcgct gcaattggtg
     2221 ctgaaacgat aaagtaattg tgtgacccag atcgatattt acagggagcc tgcctttccg
     2281 gcgttgttgt tatgccccca ggtatttaca gtgtgagaaa gaattatttt gactttagcg
     2341 gagcagttga agaatgacaa agtatgcatt agtcggtgat gtgggcggca ccaacgcacg
     2401 tcttgctctg tgtgatattg ccagtggtga aatctcgcag gctaagacct attcagggct
     2461 tgattacccc agcctcgaag cggtcattcg cgtttatctt gaagaacata aggtcgaggt
     2521 gaaagacggc tgtattgcca tcgcttgccc aattaccggt gactgggtgg caatgaccaa
     2581 ccatacctgg gcgttctcaa ttgccgaaat gaaaaagaat ctcggtttta gccatctgga
     2641 aattattaac gattttactg ctgtatcgat ggcgatcccg atgctgaaaa aagagcatct
     2701 gattcagttt ggtggcgcag aaccggtaga aggtaagcct attgcggttt acggtgccgg
     2761 aacagggctt ggggttgcgc atctggtcca tgtcgataag cgttgggtaa gcttgccagg
     2821 agaaggcggt cacgttgatt ttgcgccgaa tagtgaagaa gagggcatta tcctcgaaat
     2881 attgcgtgcg gaaattggtc atgtttcggc ggaacgcgtg ctttctggcc ctgggctggt
     2941 gaatttgtat cgcgcaattg ttaaagccga caaccgcctg ccagaaaatc tcaagccaaa
     3001 agatattacc gaacgcgcgc tggctgacag ctgcaccgat tgccgccgcg cgttgtcgct
     3061 gttttgcgtc attatgggcc gttttggcgg caatctggcg ctcaatctcg ggacatttgg
     3121 cggcgtgttt attgcgggtg gtatcgtgcc gcgctttctt gagttcttca aagcctctgg
     3181 tttccgcgcc gcatttgaag ataaagggcg ctttaaggaa tatgtccatg atattccggt
     3241 atatctcatc gtccatgaca atccgggcct tctcggttcc ggtgcacatt tacgccagac
     3301 cttaggtcac attctctaaa tccttcctgt tatatcggga ggtaactctc ccgataatct
     3361 tttaaatcat acagttaatt aaatttttct ttgtgtcccc tcacaaggcc gacctgcgtc
     3421 acacttccgt acagcgggat taattctcca gtaaatgcat tatttgtctg gtaacggcga
     3481 tttgttttgc acgttcataa tttcactcgt caactatcac tgaacgagga attaacgatg
     3541 agtaagaaac tgattgcctt atgtgcctgc ccaatgggcc tggctcacac ttttatggcc
     3601 gctcaggcgc tggaagaagc ggcggtagaa gccggttatg aagtgaaaat tgaaactcag
     3661 ggcgcggacg gtatccagaa tcgcctgacg gcgcaggata tcgccgaagc gaccatcatc
     3721 atccactccg tggcagttac cccggaagat aacgagcgtt tcgaatcacg cgacgtttat
     3781 gaaatcactt tgcaggacgc aattaaaaac gctgcgggaa tcatcaaaga aatcgaagag
     3841 atgattgcct ctgaacaaca gtaatattcg acagggataa ttgttatggc cattaaaaaa
     3901 cgcagtgcaa ccgttgtgca tggcgcatcc ggtgcggcag cggcagttaa gaatccgcag
     3961 gcctctaaat ccagcttctg gggtgaactc ccgcagcatg tgatgtcagg gatttcacgc
     4021 atggtgccga ccttaattat gggcggtgtg atcctcgctt tcagtcagct gattgcttat
     4081 agctggctta aaattcctgc tgatatcggc atcatggatg ctcttaatag cgggaaattc
     4141 tccggtttcg acctctcctt actgaaattt gcctggctgt cgcagtcctt cggcggtgtg
     4201 ttgtttggtt ttgccattcc gatgtttgct gcttttgtgg cgaactctat cggtggcaaa
     4261 ctggcgttcc cggctggttt tatcggcggc ttgatgtcta cccagccgac gcaactgctg
     4321 aacttcgatc ccagcacgat gcaatgggcg acctcttcgc cggtgccgtc caccttcatt
     4381 ggtgcgctga ttatttctat cgttgccggt taccttgtga aatggatgaa ccagaaaatc
     4441 cagttaccgg atttcctgtt agcgttcaaa accacatttt tgctaccgat tctttccgcc
     4501 atttttgtca tgctggcgat gtactacgtc atcaccccct ttggtggctg gatcaacggc
     4561 ggtatccgta ccgtgctgac cgctgcaggt gagaagggcg cgctgatgta cgcaatgggg
     4621 atcgccgcgg cgacagcaat cgaccttggt ggcccgatca acaaagcggc aggtttcgtt
     4681 gccttcagct ttaccactga ccacgtatta ccggtcactg cacgttctat cgctatcgtt
     4741 attccgccga ttggtctggg tctggcgacc attatcgatc gtcgcttaac tggcaaacgc
     4801 ttgttcaacg cccagcttta tccgcagggt aagaccgcga tgttccttgc ctttatgggg
     4861 atcagtgagg gtgcgattcc gtttgcactg gaaagcccta tcaccgccat tccgtcgtat
     4921 atggttggcg cgattgtcgg ctcaaccgcc gcagtctggc tgggcgcagt gcaatggttc
     4981 ccggagtccg ctatctgggc atggccgctg gtcactaacc tcggcgtcta catggcgggg
     5041 atcgcgctgg gggcagtcat aactgcgctg atggtggtgt tcctgcgtct gatgatgttc
     5101 cgtaaaggca aattgttaat cgatagcctg taagaaggaa aaatccagat gacattactc
     5161 gcttcgctgc gcgactggct taaggcgcaa caactggatg cggtgcttct ctcctcacgg
     5221 cagaacaaac agccgcatct ggggatctcc accggatcag gttatgtggt gattagccgt
     5281 gaaagtgcgc acattctggt ggattcgcgc tattacgcgg atgtagaagc ccgcacgcaa
     5341 ggttaccagc tgcatttgct cgacgcgatg cacacgcttg ccaccatcgt cagacaaatc
     5401 attgccgatg agcagttgca aacgctcggt tttgaaggcc agcaggtgag ttgggaaacc
     5461 gcgcaccgct ggcagttcga actcaatgcg aaactggtta gcgccacgcc ggatgtgctg
     5521 cggcaaatca aaacgccaga agaggtggag aaaatccgcc ttgcctgcgg gattgccgat
     5581 cgtggcgcag agtatattcg ccgctttatt caggcgggga tgagcgaatg cgagatagcc
     5641 gctgaactgg agtggtttat gcgccagcag ggcgcagaaa aagcctcttt cgataccatt
     5701 gtcgccagtg gctggcgtgg ggcgctgccg cacggtaaag ccagcgacaa gattgttgca
     5761 gcgggcgagt ttgtcactct cgatttcggt gcgctgtatc agggctactg ctctgatatg
     5821 acgcgcacct tgctggtgaa tggcgaaggg gtgagcgccg aatctcaccc gctgtttaac
     5881 gtctatcaga ttgtcctgca ggcacagctg gcggcaatct ctgtaattcg ccccggcgtg
     5941 cgctgccagc aggttgacga tgccgcgcgc cgcgtgatta ccgaggccgg ttatggcaag
     6001 tttttcggcc ataacaccgg tcatgctatc ggtattgaag tccatgaaga gccgcgtttt
     6061 tcaccgcggg acaccacgac gctacagcca ggtatgttac tgaccgtgga gccagggatt
     6121 tatttgccag ggcaaggggg cgtgcgcatc gaagatgtcg tgctggtcac cccgcaaggc
     6181 gcagaagtgc tctacgccat gccgaaaaca gtgttgctca cgggagaggc ataatggatt
     6241 tatcgctctt aaaagcgttg agcgaggcag atgcgatcgc ctcctcggaa caggaagtac
     6301 ggcagatcct gctggaagaa gcggatcgcc tgcaaaaaga agtgcgattt gatgggctgg
     6361 gatcggtgct gatccgactc aatgagtcga caggtccgaa ggtgatgatc tgtgcgcata
     6421 tggacgaagt gggatttatg gtgcgcagca tctcccgcga aggggcgatc gatgtgttgc
     6481 cggttggcaa tgtacgcatg gctgcccgcc agcttcagtc ggtgcgtatc actacccgtg
     6541 aagagtgcaa aattccaggc ctgcttgacg gcgaccggca ggggaatgac gtcagcgcca
     6601 tgcgcgtgga cattggcgcg cgctcctgtg acgaagtgat gcaggcggga attcgtccag
     6661 gcgatcgcgt caccttcgat accacttttc aggttctccc tcaccagcga gtgatgggga
     6721 aagcctttga tgaccgcctc ggttgctatc tgctggtgac gttactgcgt gagctgcacg
     6781 acgccgaact gcctgcggaa gtgtggctgg tggcaagttc cagcgaagag gtgggattac
     6841 gcggcgggca aactgccacc cgcgcggtgt cgccggatgt cgccattgtg cttgatactg
     6901 cctgctgggc gaaaaacttt gattatggcg cagctaacca tcgccagatt ggtaacgggc
     6961 cgatgctggt gttaagcgac aagtcactga ttgcgccgcc aaaacttacc gcctggatcg
     7021 aaaccgtggc ggcagaaatt ggcgtaccgt tgcagtcaga tatgttcagc aatggcggca
     7081 cggacggtgg ggcggtgcac ttaaccggca ctggcgtacc cacagtggtg atggggcctg
     7141 caacccgcca cggacattgc gccgcatcga ttgccgactg ccgtgacatt ttgcagatgg
     7201 agcaactttt atctgccctt attcaacgtc ttacgcgtga gacggttgtt caactgacgg
     7261 atttcagatg atctcctgat taacccggag cggttatgtt aacgattcaa tttctctgtc
     7321 ctttgcctaa cggtctacat gctcgtccgg cgtgggaact taaagagcag tgcagccagt
     7381 ggcaaagcga aatcactttt attaaccatc gccagaacgc aaaggccgat gcgaaaagct
     7441 cgctggcgct gattggcact ggcactttat ttaatgacag ttgcagcctg aacattagcg
     7501 gtagcgatga agagcaggcg cggcgcgtgc tggaagagta tatccaggtg cgctttatcg
     7561 acagcgacag cgtccagccg acgcaggcag aactgacggc gcatccgctg ccgcgttcat
     7621 taagccgcct gaacccggat ttactgtacg gcaacgtgct ggcaagcggc gttggcgtgg
     7681 gtacactgac cctgttacag agcgacagcc tcgacagtta tcgggcaata ccggccagtg
     7741 cgcaagattc caccctgctg gagcacagtc tggcaacgct tgccgagcaa ctgaaccagc
     7801 aattgcgtga gcgtgacggc gaaagcaaaa ctatcctcag cgcccatttg tcgctgattc
     7861 aggatgatga atttgcaggc aatatccgtc acctgatggc agaacagcat caggggctgg
     7921 gggcggcgat catcagcaat atggagcagg tttgtgccaa actttctgct tctgccagcg
     7981 attatctgcg cgagcgggtc agcgacattc gcgatatcag cgaacagttg ctgcatatca
     8041 cctggccgga actaaaaccg cgcaataatc tggtgcttga aaaaccgacc attctggtgg
     8101 ctgaagattt aaccccaagc cagtttttga gcctcgattt aaaaaatctt gcgggcatga
     8161 ttctggagaa aaccgggcgc acctcgcata ccctgattct ggcccgcgct tcggcgatcc
     8221 cggtactgag cggcctgccg ctggatgcga ttgcccgtta tgccgggcaa cctgcagtgc
     8281 ttgatgccca gtgcggcgta ctggcaatta atcccaacga tgcggtgagc ggttattacc
     8341 aggtggcgca gacgctggcg gataaacgcc aaaaacaaca ggcgcaagct gccgcgcagc
     8401 tggcctattc ccgtgataaa aagcgtattg atattgcggc gaatattggc acagctctgg
     8461 aagccccagg cgcgtttgcc aacggagcgg aaggcgttgg gctgtttcgt accgaaatgc
     8521 tctatatgga tcgcgacagc gcgccggacg agcaggaaca gtttgaagcg taccagcagg
     8581 tgctactggc ggcgggcgac aagccgatta tcttccgcac gatggacatc ggtggcgata
     8641 aaagcattcc ttacctcaac attccccagg aagagaaccc gttcctcggc tatcgcgcgg
     8701 tacgtattta cccggaattt gctggcctgt tccgtactca attgcgggcg attttgcgcg
     8761 ctgccagttt cggcaacgcc cagttgatga tcccaatggt ccacagtctc gatcagatct
     8821 tatgggtgaa gggtgagctc caaaaagcga tcgttgagct taagcgcgat ggcctgcgtc
     8881 atgcagagac gattacgctg ggtatcatgg tggaagtgcc gtcggtgtgc tacatcatcg
     8941 accacttctg cgatgaggtc gatttcttca gtatcggttc taacgatatg acccagtatc
     9001 tgtatgcagt cgatcgtaat aacccgcgcg tatcgccgct gtataacccg attacgccat
     9061 cgttcctgcg catgttgcag cagattgtta ccgctgcgca tcagcgcggc aaatgggtag
     9121 gtatttgcgg tgaactgggc ggtgaaagtc gttatctgcc gctactgcta gggctgggcc
     9181 tggatgagct aagcatgagt agcccgcgta ttccggcagt gaaaagccag cttcgtcaac
     9241 tggatagcga ggcgtgtcgg gaactggcgc gtcaggcgtg cgagtgccgc agcgcgcagg
     9301 aaattgaagc gttacttacc gcgtttgcgc cggaagaaga cgttcgccca ctgctggcac
     9361 tagagaatat ctttgttgat cagtccttta gtaataaaga gcaggcgatc cagttcctgt
     9421 gcggtaacct cggtgttaac gggcgtactg aacatccgtt cgagctggaa gaagatgtct
     9481 ggcagcggga agagattgtt actactggcg ttggttttgg cgtggcgatc ccgcacacca
     9541 aatcacagtg gatccgtcat tccagtatca gcattgcccg gttggtgaaa ccggttgact
     9601 ggcagtcaga aatgggcgaa gtcgaactgg tgatcatgct gacgctgggc gctaacgaag
     9661 ggatgaatca tgtgaaagtc ttctcgcagc tggcgcgtaa actggtgaat aaaaacttcc
     9721 gccagtcgct gtttgccgcg caagatgcac aaagtattct gacgctgctg gaaacggaat
     9781 taaccttctg acgttagccc tgaaaacgtg cgctatactc tcccggcgtc agaccaaact
     9841 gatgccggaa aacgcgacaa aaatagtcgc tatccggaaa cccgcaacgc tgcgccactt
     9901 catgaatcga cagatgatat ttttgcaaaa tcatcctcgc ttttgccatt cgtacccagc
     9961 gaacatactc gataaaacgc attgttccat gctgagcaaa cagttttgac agatgattgg
    10021 gcgtgatatt aaaaaactgc gccacgctct cgcgggtgag cggctgggca taattatcct
    10081 gaatccagtt acaaatgctg tgatagagaa aatcgccgcg cggttgcgaa ttgcccggcg
    10141 gcatattcac cacgttacgg caaagatgca gcaaacttaa caccagcggc tgaatgatat
    10201 tctgctccag tggcgaacgg ctaagatggg tgagggcggt aagcatcgct tcaccttcac
    10261 cgcgttgcag gctgggaagc tggatctggc gagcaggatg taacaacgac gtggtgcggc
    10321 tatcgtaaaa cgataaccca agccaggtcg gtgcgaacac caggcttaac agcatcaccg
    10381 gtttgttgtt gaccggtaaa ttagccgcgc gtgccggaat aaacagcatt tctccttcca
    10441 ccatacgttg ctgctttgct tccagcagat tgccgtattc gccgcgtagc acgatatcca
    10501 gacggggaaa atcaatacat aaactgccca ctggcaatga ggcgggctgg ctggcaaacc
    10561 agacgcgccc aaggagttgc gggttaagca ccaggccgct gaacagatcg gcgaaaaatt
    10621 gttgatcggc gggcaagcct ggtgctttca tgaaagttcc cgattacaga tgcattaact
    10681 ggcggaattc tttaactttg ctacggctga ccggcacttc aaaatccaga tctttcagcc
    10741 gcagaatgta ggtgttatta aaccacggtt cgatttcgcg tattttgttc agattgacac
    10801 aaaatgagcg atggcagcgg aaaaaatgcg acggcggcag cttgctgcaa aactcggtaa
    10861 tgttcatcgg cattacgtag gattcacgcc gcgtatagac aaaggtcatt ttctcgtgcg
    10921 cttcggcgta atagatatcg ttaattggcg tgacgatgat tcgctcatct ttcaccagat
    10981 taatggtgtc attctcacgc gttaccgtcg cggcaggcgt cgtactgctg gtctgctgtt
    11041 gttgccaggc tgcttccagt ttttgcagca tcccggtaat gcgtgattcc tgatacggtt
    11101 tgagaatgta gtcaaacgct tccagttcaa atgcttccac cgcatgttct ttccacgcgg
    11161 tgatgaacac aataaacggt ttatgggcga actggctgat gttttgcgcc agcaacacac
    11221 cgtccagcga cggaatattg atatccagaa aaatggcgtc gacgcggtta tgctgcaaaa
    11281 acttcaacac gtccagaccg tcgtcaaagg tgccgacaat ctccatctgg ctgtgctctt
    11341 taattagcca gctcagttcc tgttgtgcca ggaattcgtc ttcaacaatg atgactttca
    11401 caatatcact ccggctcaaa gcaataaggt agcctgtgag gcgactgggg tgcgttggtt
    11461 aggaatgtaa aacgcaattt ccgttcctgg ttccaggcgg cggatatgca gcccctcgcc
    11521 atacaataac ttcacgcgat gatggacatt cagcaggcca attttattgc ccggcatttc
    11581 gttcgcttcg acccgctcaa tcacctttgg atcgatgccg tgcccggtat ctcgcaccgc
    11641 aatacgtacc cgattaccgc actctgcaac gctgatggtg acaacgcctt tacctttgca
    11701 aggctggata ccgtggacaa tggcgttttc caccaacggc tggatcagca ggctgggaat
    11761 gcagcaattc acctcttcat caatatcata gataaccgtc agcttgtcgc caaagcgggc
    11821 ctgctcaatg gcaatatagt ctttaatttg atacagctct tttttgatat cgatttgctc
    11881 atcgtctttt aattcaatgt tatagcgcag ataacgcgac agattaaaga tcagctggcg
    11941 agcggtatcc ggattcagac ggattgacga tgaaatagcg ttcaaagcgt taaacaggaa
    12001 atggggatta attttgcttt gcagggcgcg aagctctgcc ttatttgcca tttcacgcag
    12061 ctgctcggcg cgtgacacct ccagttgcgt ggagatgatt tgtgacagac cgaccgccat
    12121 ttcctgtaat gacgaggtta tctgatgcgc gtggcagtag taaattttca gcgttccggt
    12181 cacaacgcct ttctcccaca atgggatcac cagcatggaa tgaatctctg gtgtgcggtg
    12241 ggcttcatca ttgtttttaa tgatgatttt tccataattc atcgcctggc gggtagtcgg
    12301 gctaatgaag tcatcgccat tctgatagtt atgttcaccc acgccaacgt aggccagcac
    12361 atgatcggta ttagtcatcg ccaccgcatc ggcgtgaatg tcatcgcgga taatttcgca
    12421 gactttgcgt aatgactcgc tattgacatg gcgaaacagc ggcagcgttt tgttggcgat
    12481 atccagcgcc agcttggcct gccgcgcggc gctggcctct ttttctccct caacactttg
    12541 taccagcaac acaataaagc cgatacagac gctgccgagg atcattggaa tcccgatttt
    12601 agagacgata tcgatcccca atgcggtagt tggtgcccag acaatcacca gaatcatggt
    12661 caacgtttca cacaacatgc cgccgagaat accgacgcgc cagcgctgtg ctttggggat
    12721 tttcaggttg atccagcccg atatacaacc cgccagaatg ctggtgataa agcaggggat
    12781 cgccgtcacg ccgccgatgt caattaaata acggtgaata cccgcaatca cgccggtaat
    12841 gatgcccacc cacgggccaa acagaatccc gcctgacatc accgcgataa tacgtacgtt
    12901 caccagcgag ccttctacgg gaacgccgga ccaggtgctg aacagggcga acagcgagaa
    12961 aatggcggta acggcgagca gctctttcgg tgagtgtgcc gacttgtgca acagttcgcg
    13021 aaacaggcgg atacggatga gaaaaaacag gcagataagc attaacgctg cccgatcgaa
    13081 gaccgccagc agcatgttga atatttcgtg cacgggtgac tctgaataag gattaaacct
    13141 ctatgatagg taacctgaag gctgatgacc agcaggcctt ttttgaggaa aaaccacaat
    13201 tttaaaaggg gattgaaaga taa
//
LOCUS       DADSZD010000122        13120 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.123, whole genome shotgun sequence.
ACCESSION   DADSZD010000122 DADSZD010000000
VERSION     DADSZD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.123"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(81..1040)
                     /locus_tag="J1Z28_003237"
     CDS             complement(81..1040)
                     /locus_tag="J1Z28_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBA7718047.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPEAARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSEGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGEASPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCY"
     gene            complement(1233..2423)
                     /gene="glpC"
                     /locus_tag="J1Z28_003238"
     CDS             complement(1233..2423)
                     /gene="glpC"
                     /locus_tag="J1Z28_003238"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBA7718048.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(2420..3679)
                     /gene="glpB"
                     /locus_tag="J1Z28_003239"
     CDS             complement(2420..3679)
                     /gene="glpB"
                     /locus_tag="J1Z28_003239"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBA7718049.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHVAQQIAQRAGGQQ"
     gene            complement(3669..5297)
                     /gene="glpA"
                     /locus_tag="J1Z28_003240"
     CDS             complement(3669..5297)
                     /gene="glpA"
                     /locus_tag="J1Z28_003240"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBA7718050.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQTLHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEATLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            5570..6928
                     /gene="glpT"
                     /locus_tag="J1Z28_003241"
     CDS             5570..6928
                     /gene="glpT"
                     /locus_tag="J1Z28_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBA7718051.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            6933..8009
                     /gene="glpQ"
                     /locus_tag="J1Z28_003242"
     CDS             6933..8009
                     /gene="glpQ"
                     /locus_tag="J1Z28_003242"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBA7718052.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            8212..8862
                     /gene="inaA"
                     /locus_tag="J1Z28_003243"
     CDS             8212..8862
                     /gene="inaA"
                     /locus_tag="J1Z28_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBA7718053.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(8916..9170)
                     /gene="yfaE"
                     /locus_tag="J1Z28_003244"
     CDS             complement(8916..9170)
                     /gene="yfaE"
                     /locus_tag="J1Z28_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBA7718054.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(9170..10300)
                     /gene="nrdB"
                     /locus_tag="J1Z28_003245"
     CDS             complement(9170..10300)
                     /gene="nrdB"
                     /locus_tag="J1Z28_003245"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBA7718055.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(10490..12775)
                     /gene="nrdA"
                     /locus_tag="J1Z28_003246"
     CDS             complement(10490..12775)
                     /gene="nrdA"
                     /locus_tag="J1Z28_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBA7718056.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         3059 a         3298 c         3514 g         3249 t
ORIGIN      
        1 ccttctaatt taccctcctg ccagccaatt tgatgtcctt cctgccgtaa ccgttcagca
       61 atagtcatta atctctcctt ttaataacag ttcggaatat cagcatcagt gagttgggtt
      121 gtcgccagca caatctcacg ttcaaagccc tgttccagca tgcgcaaggc aattttaatg
      181 gcttgttcat gcataccttc tactttgcct ttctgcaaac cctccaattt cccctcctgc
      241 caaccttcaa ttttaccttc ctgccagcca atttgatgcc cttcctgccg cagccgttca
      301 gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt cggcaatctc ctgaataaaa
      361 cgggtgaaac gggaggtatc gccgcattgc agcagataat taaacagtgt ttgtagctga
      421 ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa
      481 tcgcggtcgc gaatatgctt ttgaatcagt tccagcagag ctatacgccg atgttgcatg
      541 atctcatcgt caggtacgat ggtgatatcc accaacggaa aagcttcggt gtacaactgc
      601 cgggcaagtt gcggatcgtc aaactcatcc agccagttga gtgagtacgg gtagggcgag
      661 gcttcgccat gataaaacag caatggcacc accagcggaa ctctgtcata gcctttatcc
      721 aggtgacgct gcatggcggc agtggcatag cgcattagcc gaaaagccat attcttttct
      781 gcagagcttt gatgttcaat cacgcagtag atatagccgt caccctcgct ggtttccaca
      841 gaccacaaaa catccgagta gtaagcgcgt aaactttttt caataaaact agtgggttcc
      901 aggcgtaagg tttgcaggtt gcaaagcttg cgcagcggct ctggtagatg tatttccaga
      961 aaatcccgtg cggcttcggg tgtgaacata aaggttttaa acaccgcatc atgcggggag
     1021 gaggttgttg attcggtcat ggttgtccgt cacctgatat ttatgatgac gcgacattac
     1081 ggttatccca ggcctttgac atctgccagt ttcagccttt gcgagacatt ttcagaggaa
     1141 ttttcactgc gttgcaacgc atacattggg cacggaatcg tgtacgcaaa aatgcccggt
     1201 aacaacatgc ttaccgggca tcagaaagga gtttaagcca gcgcctgtgc cagcagcgta
     1261 atcggatgtt cgcagcgaag acttgtggac atctcaatct gccatttaca ggtttcgcag
     1321 tcggtgacca ccagatctgc gccgctctct tctatctggc ggaacagtgg tgcgccgatg
     1381 gcttgtgaag tggggtagtt ctcttttttg aaaccgtaag ttcccgcaat accgcagcac
     1441 tgggaatcca gcaccgttaa ctcaagcccc gggattttac gcaacagctc cagggtgtag
     1501 agcgtccagc ccattttttc catatggcac ggagtgtgat aaaccacttt cagcggcagc
     1561 ggtttcagcg gtaacgtttt gccttcgtcc agcttgcgcc acagccagcg ggtcgccagt
     1621 tcgatatgat cgcgcaagcc tttgttgtcg acattcagca cttccgggta ttcgtcgcgc
     1681 agggcaaatg tacacgttga ggaggtggca atcaccggaa tgccttttac tcccacagct
     1741 tcgcggatcg actctacatt cgtaattgcc tgtttgcgtg ctttatcggt aaaaccgttg
     1801 gcgatcagcg gtacgccgca gcatttttct ttgctgagca gttgcacgcc ggtacccatc
     1861 gcgttgagca ctttaattaa atctttgcct agctgcggat ggttgtagtt aacgaagcag
     1921 ccgtgaaaga aagcgacctg gtctttatac tgcgcttgct gagccgccac gctgcgatac
     1981 cagcgacgga acgtaccgaa ggagtatttc ggtagcgtgc gacggtggtc gatttttaac
     2041 gccgcatcaa gcaactgccg caccggtttc agcgaggtag cagtgttgac gatgggggcg
     2101 aacggcgtgg aaacgctacc catcaggtcg gtatgactca acacaaaatt acgcagcgac
     2161 gggcgtgtgg tgtcatattt cgcccgcgcg cgctggataa tatcgccaat cttcacatcg
     2221 gacgggcagg cgacttcaca acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac
     2281 agtgcaccat cttttaaacg cagacgctcg ccatccggcc cggcttgttt tggccccgga
     2341 taacccggat tcacccggct caccgggcag gcggtggtgc agacggtgca cttaatgcag
     2401 ttttcgaagc tggtgtcatt cattgttggc ctcctgcgcg ttgggcaatc tgttgagcga
     2461 catgtaaagc actgacggca caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc
     2521 cgcccagcac cgaaccgatg gcaaacaggt tctcaatggt ttgccccgcc tgcgacgggc
     2581 gtagcgcctc atcagtggtt acaccgaact gctgccacgg ctgcggcgca aaaaaatcgc
     2641 ccttatacca ttcaccccgc gtagcggttt gtagcacatc aaggccgaga atcggctcgc
     2701 gaatgccgtc acgttctgcc accagtccgc cgctaaagaa actgccgctc gcaagcaccg
     2761 caaatcgtgg gcgcagcggg atatcggcgt gattgcgcgt ccagatttca ttcactacac
     2821 catttttaca ggtcactttt ttcacttcat cgcccggcat ccacacgcca ccctggcgca
     2881 caaactggcg ctgtaactgg ttttgcagac gaatgcccag cacggaaggc ggcagcgttg
     2941 gcaaaagcat caatgaacaa ggtagttttt cattcaacca atgccacagt ttgtcatcgg
     3001 ccaggccgaa gcaggcgggc atcaggatca tttcgcaggt attggcgaca ggaataagcg
     3061 catcaagtaa caacggccag ttttcttcat tatcaaggaa acgggcgata ttcaccgccc
     3121 ggaactcggt ggcgttattg cgcagcacat ccagttccgg cagttcaatt tcagcggttt
     3181 caactttaag atccaattcg cgcaatgaag ccgccgcaag atgcgcctga aaatccatca
     3241 ggccgctaat tcccactaca catattttct tcgcgggcag cggccagaca gggacttctg
     3301 gcgaacttag ccaggttgag cgcagagtgc ctagcggtgt aatccgctgg tgagccagct
     3361 ctacgctgcc ctgcaattgc gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga
     3421 gatcgagcac gcgttgaggc ccgagaagag tgtaaggatg ggcaggtgcc tgctgacgca
     3481 aagattccag tccactgtga atgtctgtca ccggttgacc atccggcaga tggctcagca
     3541 aatccagcga tccagatgag aaatggagcg cgctttgacc acgagtgaca atggcacagc
     3601 gcaggccgtg tttttgcagt tgcaggccac agagtaatcc ggcgaggccg ccgcccataa
     3661 tgacagtatc aaagcgcatc tttctgctcc ttctccagac cacacaatcc ctgataaacc
     3721 cagcgggtaa attcgctttc gcgcagtgca tctccccagg cgatgggttg cacgcctttc
     3781 cagcgttcgt ttaggaaggt ggaaagttgc tcgatagatt gtgcggacgt cgtgacgtta
     3841 aaacgttgca gcagtccggc agcgcggcag gcgcagagtt ctccctggca ggtgcccatc
     3901 cccacacggg tacgacggcg taaatccagc aggctattaa cgtttaaatt ttctaccgcg
     3961 tactgcactt cacccgcagt taccgcttcg cactcacata ccaggctacg gtgcagacgg
     4021 ccttcgctca gccaggcagg cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc
     4081 ggggcaggta gggagatgac tttacgcaag gtagcttcag cgggttcctg tgaaccagga
     4141 agtgccagat cggcggtcgt acaggggcgc gtgttgccca gtttgcggca tacggcgtcg
     4201 gtagcccatt ccgccattag ccgataggtc atcagtttgc caccggtgat ggtgataaac
     4261 ccgtccagac catcgcgttc agcatggtcg agcagcacga tgccacggct gacgttacgt
     4321 ccgctcggat cgtcatcgct ggcaaccagc gggcgtacgc cagagtaggc ccgcaaaatg
     4381 cgcgttttcg ccatcacggg ggccagtttt tccccttcac gcagcagaat atcaacttct
     4441 tctgccgtca ctcgattatc gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca
     4501 atcagcgaaa tggtatcgcc aggcaccaga atatcggcgt cggaaggttt gcggcagcgg
     4561 ttgatcacat gctgattaat gcggtgatcc atgatcagca gcgatccttt cgccgggaac
     4621 atgcgaatgc gcagatcggc atattcggca atgtgttgcc cccagatccc ggcggcatta
     4681 actacgacag gcgcatgaag tgtctgaatt tcgccggtga gatggttacg tacacgaaca
     4741 ccgcacaccg tcgcgccttc acgaatcagc cccgtgactt catgagcggt aaggataacg
     4801 gcaccgtgtt ctttggcatc cagcatgttt gctgctgtca gacgaaatgg atcaacggtg
     4861 ccatccggaa ctttcaccgc gccaatcagt gccgggttaa cggcaggttc gataatgcgc
     4921 gcttgctgcg gatcaatggc ttctgcgctg atccctgctt cttcgcaggc gcggataaaa
     4981 gtggcctgga aggagaggtc atcttccggc agggtgataa acaggccgtt ggttggttct
     5041 acgcagtgac gtgcaatgcg tttcaggatc tggttttcac taatacattc gcgggccgat
     5101 tccgcatcgg ttaccgcata gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg
     5161 gcaccggttg cgatgtcgtg gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag
     5221 tcgcgggcaa tcccggctcc cgttgcgcca ccgccaatga taatcacgtc acttgattgc
     5281 gagtcgcgag ttttcattgt tatccctctg aagttcgttt tttaccattt agccatagta
     5341 aaaacatgaa ttgtttgatt tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt
     5401 tcatgcgtca ttctgaacat ttcaaccatc gtatttaata atgtgtgcgg taattcacat
     5461 ttaatttatg aatgttttct taacatcgcg gcaactcaag aaacggcagg ttctcccact
     5521 gaatcaggct gttaatcata aataagacca cgggccacgg aggctatcaa tgttgagtat
     5581 ttttaaacca gcgccacaca aagcgcgctt acctgccgcg gagatcgatc cgacttatcg
     5641 tcgattgcgc tggcaaattt tcctggggat attctttggc tatgctgctt actatttggt
     5701 tcgtaagaac tttgcgctcg ctatgcctta tctggttgag cagggattct cacgcggtga
     5761 tttaggtttt gccctttcgg ggatctcgat tgcttatgga ttttcgaaat tcatcatggg
     5821 ttcggtatcg gatcgctcga atccgcgcgt tttcctgccc gcaggtttga ttctggcggc
     5881 ggcagtgatg ttgtttatgg gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt
     5941 tgtactgttg ttcctctgcg gttggttcca ggggatgggg tggccgccgt gtggtcgtac
     6001 tatggtgcac tggtggtcgc agaaagaacg tggcggtatt gtgtcagtgt ggaactgtgc
     6061 gcacaacgtc ggtggtggta ttccgccgct gctgttcctg ctggggatgg cctggttcaa
     6121 tgactggcac gcggcgctct atatgcccgc tttctgtgcc attctggtgg cactgttcgc
     6181 ctttgcgatg atgcgcgata ccccgcaatc ctgtggcctg ccgccgatcg aagagtacaa
     6241 aaatgattat ccggacgact ataacgaaaa agcggaacag gagctgaccg cgaagcagat
     6301 cttcatgcag tacgtgctgc cgaacaaact gctgtggtac atcgccattg ccaacgtgtt
     6361 cgtttatctg ctgcgttacg gcatcctcga ctggtcaccg acttatctga aagaggttaa
     6421 gcatttcgcg ctggataaat cctcctgggc ctacttcctt tatgaatatg caggtattcc
     6481 gggcaccctg ctgtgcggct ggatgtcgga taaagtcttt cgtggtaacc gtggggcaac
     6541 cggcgtcttc tttatgacac tggtgaccat cgcgactatc gtttactgga tgaacccggc
     6601 aggtaaccca accgtcgata tgatttgtat gattgttatc ggcttcctga tctatggccc
     6661 tgtgatgctg atcggtctac atgcgctgga actggcaccg aaaaaagcgg caggtacggc
     6721 agcgggtttt accgggctgt ttggttacct cggcggttcg gtggcggcga gcgcgattgt
     6781 tggctacacc gtggacttct tcggctggga tggcggcttt atggtaatga ttggcggcag
     6841 cattctggcg gttatcttgt tgattgttgt gatgattggc gaaaaacgtc gccatgaaca
     6901 attactgcaa aaacgcaacg gaggctaatg gcatgaaatt gacgctgaaa aaccttagca
     6961 tggcgatcat gatgagcact atagtcatgg gaagcagtgc aatggcggcg gacagcaacg
     7021 aaaaaatagt catcgcccat cgcggtgcca gtggatattt gccggagcat acgctgccag
     7081 caaaagcgat ggcgtatgcg cagggagcgg attatctgga acaggatttg gtgatgacca
     7141 aagacgacca tctggttgtt ctgcatgacc attatctcga tcgtgttact gatgttgccg
     7201 atcgtttccc ggatcgggcg cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg
     7261 atgaaattaa gtcgctgaaa tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc
     7321 agacttatcc ggggcgtttc ccaatgggta aatctgactt ccgggtgcac acctttgaag
     7381 aagagattga atttgttcag gggttaaacc actctaccgg gaaaaatatc ggtatctatc
     7441 cagaaattaa agcgccgtgg ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc
     7501 tggaagtgct gaagaaatat ggttacaccg gtaaggacga caaagtttat ttgcaatgtt
     7561 ttgatgctga tgagctgaag cgtattaaga atgtgctgga acccaaaatg ggtatggatc
     7621 tcaatctggt acagctgatt gcctataccg actggaatga aacgcagcag aaacagccgg
     7681 acggaagctg ggttaattac aattacgact ggatgtttaa gccgggggct atgaaacagg
     7741 tggcggaata tgctgatggt attggtccgg attaccatat gttgattgag gagacatcgc
     7801 agccgggtaa tatcaaactc actggcatgg tgcaagatgc tcagcagaat aaactggtag
     7861 tgcatcctta taccgtgcgg tcagataaac tgcctgaata cactactgat gtgaatcagt
     7921 tatatgatgc tctgtataac aaagcgggtg taaatgggct atttactgat ttccctgata
     7981 aggcagtaaa attccttaat aaagagtaaa ttaactaccg gatggcactt atgtgctatc
     8041 cgataccact cttcattaaa gtctgtgggt tatccttaat tgcaattatt tagagcgaga
     8101 tgacttgttc ccggcataag atactcgtca atacaacacg tattattaag attttcctca
     8161 gctaaacatc tatagtcatt gtcgttaata aaaaacgaca ggcgataggg tatggcagtt
     8221 tcagcaaagc acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag
     8281 cctaactatc gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag
     8341 ctgtatgtaa agcgcatgac gcatcatctg tttcattccg tacgttatcc gttcggccga
     8401 ccaacgattg tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg
     8461 ccgaagatcg tttttggtga agcggtgaaa attgagggtg aatggcgcgc gttgctggtg
     8521 actgaagata tggcggggtt catcagcatt gctgactggt atgcccggca tgcagtatcg
     8581 ccttattctg acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa
     8641 atgcatagcg ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca
     8701 gaaggtaagg cagaagctgg ttttcttgat ctggagaaaa gtcgtcgccg cttgcgtcgt
     8761 gataaagcga ttaatcatga cttccgccag ctggaaaaat atctggagcc gatccccaaa
     8821 gcggactggg agcaggtgaa agcatattac tacgctatgt aatcgttgta ggccagataa
     8881 gacgtataag cgtcgtatct ggcgttaaaa ccaattcaca tctcgatttc aatatcgcct
     8941 tttgcccggc aacaacaggg caaaatttcc cccggctgaa taaaagctaa tggttcggca
     9001 atccagtcca cctggcctgc gaccagtcgg gtgcgacagg agccgcagta accttcgcgg
     9061 cactggtact caaccgccac attgtgggat tccagcgccg ccagaagcga agggtgttca
     9121 tcctggcaca gcagttgtgt gccagtaatg cgcagggtaa cgcgggccat cagagctgga
     9181 agttactcaa atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga
     9241 cttccacttc ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccacg
     9301 ggatcgggtt ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat
     9361 tggtgatgta ttcaacgtac tggcagagga tgtctttatt cagaccaatc atcgaaccat
     9421 cacggaacag ataatccgcc cagtctttct cttgctgtgc cgcctggaca aacaggtcat
     9481 agcactcctg cttacattct tcggcaattt ccgccatctc cggatcatcc gcgccgctgc
     9541 gcagcagatt cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca
     9601 ggcgaataat tttggcgtta ccttccatca gctcacgttc agcgaatgcg aaagagcagg
     9661 caaagctgac gtagaaacgg atcgcttcca gcgcgttaac gctcatcagg cagagataca
     9721 gttttttctt cagctcgcgc aggctaacgg ttacggtttt accgttaacg gtgtgggtgc
     9781 cttcgcccag cagatgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga
     9841 tcccttccgc acgtttctgg atctgctcgt tggtgacgat atcgtcaaag acaactgacg
     9901 gatcgttaac gatattacgg atgatatggg tataggagcg ggaatgaatc gtttctgaga
     9961 acgcccaggt ttctacccag gtttccagtt ccgggatcga gatcagcggc agcagtgcca
    10021 cgttcggact gcgaccctga atggaatcca gcagcgtctg atatttcagg ttgctgataa
    10081 agatgtgttt ttcgtgttca ggcagtgcct ggtaatcgat acggtcgcgg gagacgtcaa
    10141 cttcttccgg acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat
    10201 atttttgctg atcgtagcga gccacgttga ccggctgacc aaagaacatt ggttctttga
    10261 gctgatcatt tttcgtctgt gaaaaggtgg tatatgccat gaatgtgtcc tgttaggagt
    10321 tttatgccgg atgcgcttcg cttatccggc ctacaaaccg catcgtaggc tgggtagggc
    10381 gttcatgccg catctgggca tccagtgccg gatgcgcttc gcttatccgg cctacaaatt
    10441 ggacccgtag gccgggtagg gcgctcacgc cgcatccggc atcacaatat cagatcttac
    10501 atgcgccgct ttcgcagcca tcgtcctgga ttgacggcac cagatcgtcc tgcgcgtctt
    10561 ctgcgccgtc acgggtgttc tgataataca gcgttttgac cccgaatttg taggcagtga
    10621 gcaggtcttt cagcaactgc tgcatcggca cttttcctga cgggaaacgt gacggatcgt
    10681 agttggtgtt agcagagatc gactgatcga taaatttctg catgataccc accagttgca
    10741 ggtagccatc gttacccggc atttcccaca gcagctcata ggcgtcgtgc agatgctcgt
    10801 agtccggcac cacttggcgc agaataccgt ctttcgacgc tttgatgctg acgtaaccgc
    10861 gcggcggttc aataccgtta gtggcgttag agatctgcga agaagtctcg gacggcatca
    10921 gggcagaaag cgtggagtta cgcaggccgt gcgttttgat tgactcacgc agtgcttccc
    10981 agtcgtaatg cagcggctca ttagcgatgg catccagatc tttcttatag gtatcgatcg
    11041 gcaggatccc tttcgcgtaa gtggtttcgt taaaccacgg gcacgcgcct tgctctttcg
    11101 ccagctcatt agacgctttc agcaggtaat actgaatggc ttcgaaggtt ttatgcgtca
    11161 ggttgttggc gctgccgtcg gagtagcgtt taccgtgctt cgccaggtag taagcgaagt
    11221 tgatcacacc aatacccagc gtacgacgac ccatcgctcc acgtttggcg gccgggatcg
    11281 ggtaatcctg ataatccagc agcgcgtcga gtgcgcgtac tgccagaatt gccagctctt
    11341 ccagttcatc caggctgtta attgcgccca ggttgaaagc agacagcgta cacagcgcga
    11401 tttcaccgtt ctcgtcgttg acgtcgttca gcggtttggt cggcagggcg atttccaggc
    11461 acaggttaga ctggcgcact ggcgcgatgg ccggatcaaa cgggctatgg gtattgcagt
    11521 ggtcaacgtt ctgaatatag atacgaccgg tagacgcacg ttcctgcatc atcagcgaga
    11581 acagctcaac ggctttcaca cgctgcttgc ggatgctgtc gtctttctca tatttggtat
    11641 acagacgttc aaactcttcc tgatcggcga agaacgcgtc gtacagcccc ggtacgtcgg
    11701 acgggctgaa cagagtgata tcttcacctt tcagcaggcg ggtatacatc agtttgttga
    11761 tttgaacccc gtagtccata tgacgcacgc ggttgccttc aaccccacgg ttgtttttca
    11821 acaccagcag gctttccact tccagatgcc acatcgggta gaacagcgtt gccgcaccgc
    11881 cgcgcacgcc gccctgagag caggatttca ccgcggtctg gaaatgtttg tagaacggaa
    11941 tacaaccggt atggaacgct tcgccaccgc gaatcgggct acccagcgca cgaatacgcc
    12001 cggcgttaat gccgatcccg gcacgctggg aaacgtattt aacaatcgcg ctggaggtgg
    12061 cgttgatgga atccaggctg tcgccgcact caatcagcac gcaggagctg aactgacgag
    12121 tcggggtacg cacgccggac atgattggcg tcggcagcga aattttaaat gtggaaaccg
    12181 cgtcgtaaaa acgcttcacg tattgcaggc gcgtttcacg cgggtagttc gagaacaggc
    12241 acgcggcaac cagaatataa aggaactgag cgctttcgta gatttcgccg gtcacgcggt
    12301 tctgtaccag atatttgcct tccagctgct taacggcagc ataagagaag gtcatatcac
    12361 ggtcgtgatc gataaacgtg tccatctgct tgaactcttc ttccgtgtag tcttccagca
    12421 gatgattatc gtatttgccc atctcgacca ttttcaccac atggtcgtac agcgcaggcg
    12481 gctcaaactg accgtaggct tttttacgca ggtggaagat cgccagacgt gcggcgagat
    12541 actgataatc cggcgcatca cgggagatca ggtctgcggc agccttgata atagtttcgt
    12601 ggatgtcaga ggtcttgata ccgtcataaa actgaatgtg ggagcgcagc tcgacctggg
    12661 aaatcgaaac gttatgcagt ccttctgccg cccaatccag aacgcgatgg attttgtcga
    12721 gattgatgcg ctctgtgcta ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg
    12781 tcgtacctgt ttttcaatag cttcatgcag actacctgta gtgagggaag ctgctaagaa
    12841 cactatatat agggggtatg tttgggattc actgcaagat agtgtgaaac tggccctctt
    12901 gcaagtgcat aactttgtgg ataactcagg aaggaaaaag tggcttccgc gcaccttagg
    12961 tcagacaagg tgtccgggaa agtcaatggg aagagaaaaa tttgttaaaa ataaccgttc
    13021 ggtgaattct tgaacggtaa aataggatag gtaaattgat atagatggtt tattaatctt
    13081 gttgctaatg attgccaaaa ttgatctagg cacaagtcgt
//
LOCUS       DADSZD010000123        12984 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.124, whole genome shotgun sequence.
ACCESSION   DADSZD010000123 DADSZD010000000
VERSION     DADSZD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.124"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            3..863
                     /locus_tag="J1Z28_003247"
     CDS             3..863
                     /locus_tag="J1Z28_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7718057.1"
                     /translation="MEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGVQLFD
                     RVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILPAVIA
                     RYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDELVVFA
                     APTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNSEAIK
                     HAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNALRRFL
                     DYCDPANVPR"
     gene            1068..2537
                     /gene="lysP"
                     /locus_tag="J1Z28_003248"
     CDS             1068..2537
                     /gene="lysP"
                     /locus_tag="J1Z28_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBA7718058.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPCNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2829..4820
                     /gene="cirA"
                     /locus_tag="J1Z28_003249"
     CDS             2829..4820
                     /gene="cirA"
                     /locus_tag="J1Z28_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBA7718059.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTVQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4852..5688)
                     /locus_tag="J1Z28_003250"
     CDS             complement(4852..5688)
                     /locus_tag="J1Z28_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase YeiG"
                     /protein_id="HBA7718060.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5946..6614
                     /gene="folE"
                     /locus_tag="J1Z28_003251"
     CDS             5946..6614
                     /gene="folE"
                     /locus_tag="J1Z28_003251"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBA7718061.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6631..7788
                     /locus_tag="J1Z28_003252"
     CDS             6631..7788
                     /locus_tag="J1Z28_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBA7718062.1"
                     /translation="MERNVTLDFVRGVAVLGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKHWKLHSGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            7930..8970
                     /gene="galS"
                     /locus_tag="J1Z28_003253"
     CDS             7930..8970
                     /gene="galS"
                     /locus_tag="J1Z28_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBA7718063.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9250..10248
                     /gene="mglB"
                     /locus_tag="J1Z28_003254"
     CDS             9250..10248
                     /gene="mglB"
                     /locus_tag="J1Z28_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBA7718064.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10309..11829
                     /gene="mglA"
                     /locus_tag="J1Z28_003255"
     CDS             10309..11829
                     /gene="mglA"
                     /locus_tag="J1Z28_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBA7718065.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            11845..12855
                     /gene="mglC"
                     /locus_tag="J1Z28_003256"
     CDS             11845..12855
                     /gene="mglC"
                     /locus_tag="J1Z28_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBA7718066.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCVIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
BASE COUNT         3095 a         3100 c         3490 g         3299 t
ORIGIN      
        1 agttggaagt ttttgcagaa gtattgaaaa gtggatcaac cacccaggcg tcggtgatgc
       61 tggcgttgtc gcaatcagca gtgagcgcag ccttgaccga cctggaaggg cagcttggcg
      121 ttcaactgtt tgatcgcgtg gggaaaagac tggttgttaa tgaacacggg cggctgctct
      181 atccgcgagc gttggcactg cttgaacagg cggttgaaat cgaacaactg tttcgcgaag
      241 acaacggcgc gattcgtatc tatgccagta gtaccatcgg taactacatt ctgcctgcag
      301 ttatcgcccg ttatcgccat gattatccgc agttgccgat tgaacttagc gttgggaata
      361 gccaggacgt gatgcaagcg gtgctggatt tccgcgttga tattggcttt attgaaggac
      421 cgtgccacag cactgaaatc atttctgaac cgtggctgga agacgagctg gtggttttcg
      481 ccgcgccgac ttcgccgttg gctcgtggtc cggtcacctt agaacagctg gccgctgcgc
      541 cgtggatcct gcgtgaacgc ggttccggca cgcgggagat tgtcgattat ctgttgctgt
      601 cacatttacc gaagtttgag atggcgatgg aattaggtaa ctccgaggca atcaaacatg
      661 cggtgcgtca tgggttggga attagttgcc tgtcgcgacg ggtgattgaa gatcaattgc
      721 aggcaggcac attaagtgaa gttgcggtcc ctctgccgcg cctgatgcgt acgttgtggc
      781 gtatacatca tcggcaaaaa cacctttcca acgcgctacg gcgctttctg gactattgcg
      841 atcccgcgaa tgtaccgcgt taaggtgctg tacaagaaca tgctggtgct gtgtcgattt
      901 cgtgacgcag cgccctcagc atgcattcgc cagaaaagag attggctgct ttacttataa
      961 tccctgggcg atcatgaagg tgtcttataa ccgtgtattt ctgccggaag gattgccaat
     1021 cgtctgctac aatcgcgcct catttttaag atggatagca tttttgtatg ggttccgaaa
     1081 ctaaaactac agaagcgccg ggcttacgcc gtgaattgaa ggcgcgtcac ctgacgatga
     1141 ttgccattgg cggttccatc ggtacaggtc tttttgttgc ctctggcgca acgatttctc
     1201 aggcaggtcc gggcggggca ttgctctcgt atatgctgat tggcctgatg gtttacttcc
     1261 tgatgaccag tctcggtgaa ctggctgcat atatgccggt ttccggttcg tttgccactt
     1321 acggtcagaa ctatgttgaa gaaggctttg gcttcgcgct gggctggaac tactggtaca
     1381 actgggcggt gactattgcc gttgacctgg ttgcagcaca gctggtcatg agctggtggt
     1441 tcccggatac accgggctgg atctggagtg cgttgttcct cggcgttatc ttcctgttga
     1501 actacatctc tgttcgcggc tttggtgaag cggaatactg gttctcactg atcaaagtca
     1561 cgacagttat tgtctttatc atcgttggcg tgctgatgat tatcggtatc ttcaaaggcg
     1621 cgcagcctgc aggctggagc aactggacaa tcggcgaagc gccgtttgct ggtggttttg
     1681 cggcgatgat cggcgtagct atgattgtcg gcttctcttt ccagggaacc gagctgatcg
     1741 gtattgctgc aggcgagtcc gaagatccgg cgaaaaacat tccacgcgcg gtacgtcagg
     1801 tattctggcg aatcctgttg ttctatgtgt tcgcgatcct gattatcagc ctgattattc
     1861 cgtataccga tccgagcctg ctgcgtaacg atgttaaaga catcagcgtc agtccgttca
     1921 ccctggtgtt ccagcacgcg ggtctgctct ctgcggcggc ggtgatgaac gcagttattc
     1981 tgacggcggt gctgtcagca ggtaactccg gtatgtatgc gtctactcgt atgctgtata
     2041 ccctggcgtg tgacggtaaa gcgccgcgca ttttcgctaa actgtcgcgt ggtggcgtgc
     2101 cgtgtaatgc gctgtatgcg acgacggtga ttgccggttt gtgcttcctg acctccatgt
     2161 ttggcaacca gacggtatac ctgtggctgc tgaacacctc cgggatgacg ggttttatcg
     2221 cctggctggg gattgccatt agccactatc gtttccgtcg cgggtacgta ttgcagggac
     2281 acgacattaa cgatctgccg taccgttccg gtttcttccc actggggccg atcttcgcat
     2341 tcattctgtg tctgattatc actttgggcc agaactacga agcgttcctg aaagatacca
     2401 ttgactgggg cggcgtagcg gcaacgtata ttggtatccc gctgttcctg attatttggt
     2461 tcggctacaa gctgattaaa ggaactcact tcgtacgcta cagcgaaatg aagttcccgc
     2521 agaacgataa gaaataagtt tcctcccttc cttgctaagc cctctcaacc gagagggctt
     2581 tttcaattcc atttccctga caaatcatgc ggatataaaa tttaacattt ggattgataa
     2641 ttgttatcgt ttgcattatc gttacgccgc aatcaaaaaa ggctgacaaa tcagaggctg
     2701 ttccggcttt ctgggatgat cacctgcata caaataagtc caccgcgatg ctgccgtacg
     2761 caaggggacg taaagaagat gtgagcgata cccattttat tttcgtagtt acctcatgga
     2821 gatatggaat gtttaggttg aaccctttcg tacgggtcgg gctgtgtttg tccgctattt
     2881 cttgtgcatg gcctgtgtta gcggtcgatg atgatggcga aacgatggtt gtcactgcat
     2941 cttccgttga acaaaacctt aaagatgctc ccgccagtat cagcgtcatt acccaggaag
     3001 acctgcagcg aaaaccggta cagaatctga aggatgtcct caaagaagtg cctggcgtac
     3061 aactgacgaa cgaaggggat aaccgtaagg gcgtaagtat tcgtggtctg gacagcagct
     3121 acaccctgat tcttgtcgac ggtaaacgcg ttaactcccg caatgccgtc ttccgccaca
     3181 atgatttcga tctgaactgg atcccggtcg attccatcga acgtattgaa gtggtccgtg
     3241 gcccgatgtc gtcgctgtac ggttccgatg cgctcggcgg tgtagtgaat atcatcacca
     3301 aaaaaatcgg tcagaaatgg tcgggcaccg ttaccgtcga taccaccgtt caggaacatc
     3361 gcgatcgcgg tgatacctat aacggtcaat tctttaccag cggaccatta attgacggcg
     3421 tgctgggaat gaaagcttac ggcagcctgg caaaacgtga aaaggatgac ccgcaaaact
     3481 caacgaccac cgataccgga gaaacgccgc gtattgaagg attctccagc cgcgacggca
     3541 atgtcgaatt tgcctggaca ccgaatcaaa atcacgattt tactgccgga tacggtttcg
     3601 accgtcagga tcgtgattcc gactcgctgg acaaaaaccg cctggaacgc cagaactact
     3661 ccgtcagcca taatgggcgt tgggattacg gcaccagcga actgaaatac tacggtgaga
     3721 aagtcgagaa caaaaaccct ggcaacagca gcccgataac ttccgaaagc aatacggtcg
     3781 acggcaaata cacgttgccg ctgacggcga ttaatcagtt tctcacggtt ggcggtgaat
     3841 ggcgtcacga caaacttagc gatgcggtga acctgaccgg gggaaccagc tccaaaacgt
     3901 ctgccagcca gtacgcgctg tttgtggaag atgaatggcg gatcttcgag ccgctggcgc
     3961 tgacgaccgg cgtgcgtatg gacgatcacg aaacctacgg tgaacactgg agtccgcgtg
     4021 cctacctggt ttataacgcc accgacaccg taacggtgaa agggggctgg gcgacggcat
     4081 ttaaagcccc ttctctgttg caacttagcc ctgactggac gagcaattcc tgccgtggcg
     4141 catgtaagat tgtgggtagc ccggatctga aaccagaaac cagcgaaagt tgggagctgg
     4201 ggctttacta catgggtgaa gaaggctggc tggaaggggt tgaatccagc gttaccgttt
     4261 tccgtaacga tgtgaaagat cgtatcagca ttagccgtac gtctgacgtc aatgctgcac
     4321 cgggctacca aaactttgtc ggttttgaga cgggcgctaa cggacggcgc ataccggtat
     4381 ttagctacta caacgttaac aaagctcgta ttcagggcgt ggaaaccgaa ctgaaaattc
     4441 cgttcaacga tgaatggaaa ctgtcgatca actacaccta caacgatggt cgtgatgtca
     4501 gcaacggcga aaacaaaccg ctatccgatc tgccgttcca tactgctaac ggtacgctgg
     4561 actggaaacc gctggcgctg gaagactggt cattctatgt ttctggtcac tataccgggc
     4621 agaaacgcgc cgacagcgcg acggctaaaa caccgggcgg ttataccatc tggaataccg
     4681 gcgcggcctg gcaggtgact aaagacgtca aactgcgcgc aggcgtgctg aaccttggcg
     4741 acaaggatct cagtcgtgac gactacagct ataacgaaga cggacgtcgt tactttatgg
     4801 cagtggatta tcgcttctga tgagaagatg cccggcgaac cgggcggact ttcacttcag
     4861 taaatactgc gcatggaagc gcaggtgatc ctctataaaa gaggcgatga agtagtaact
     4921 gtgatcatat cccggctgaa tacgcagcgt catcggccac gctttctggc gcgcggcttc
     4981 tgccagtact gcaggttgca gttggtcagc aagaaactga tcattatcac cctgatcaat
     5041 aagcgtcggg attgcatcct gcgcgttact ggcatacatc aacgcgcaac tgtcccattc
     5101 cagccatgca tttttgtcct cacctaaata agtgctaaac gctttgattc cccacgggac
     5161 gctgcacgga ttcacaattg gcgcaaaggc ggaaacgctg gtgtatttac ccggattttt
     5221 cagcgccata atcagcgcac cgtgaccacc catggaatga ccgctaatag cgcagcggtc
     5281 gctgacatta aattgcgact gaaccagcgc cggtaattca tcgcgcagat aatcatacat
     5341 ccggtaatgc gtcgcccacg gtggttgcgt ggcgttaaga taaaaccccg cgccctggcc
     5401 taaatcgtag ccatcgtcgt tggcaacctg ttcgccgcgc ggactggtgt ctggcatcac
     5461 cagtacaatc cccagttccg ccgccacgcg ctgggcaccc gccttggtgg tgaagttctc
     5521 gtcattgcag gttaatccgg aaagccagta cagcactggt ggcggggtgt gatcacgagg
     5581 tggtgggaga aagatactga acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca
     5641 gcgttgctgc cagccttcaa aacagcggtg ctcttcgagc atttccatgc gtggctcctt
     5701 gttgtgttgt ttgcaatttc ttcatcatac cgtttattca atgtgctgtg agtaactttc
     5761 acttccgtat ttgcataacg atgttttaac atctgctgat gaaaggcagc ggcaattaca
     5821 ataattatcg ctgtgaatac tggattatgt gcgccgcctc acgcacaata atcaggctgt
     5881 aaatcagctt aataactttg cccccacgca gggcggaggc gtcacacctg caggagaaat
     5941 cataaatgcc atcactcagt aaagaagcgg ccctggttca tgaagcgtta gttgcgcgag
     6001 gactggaaac accgctgcgc ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc
     6061 ttattgctgg tcatatgacc gaaatcatgc agctgctgaa tctcgacctg gctgatgaca
     6121 gtttgatgga aacgccgcat cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc
     6181 tggattacgc caatttcccg aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa
     6241 tggtcaccgt gcgcgatatc actctgacca gcacctgtga acaccatttt gttaccatcg
     6301 atggcaaagc gacggtggcc tatatcccga aagattcggt catcggtctg tcaaaaatta
     6361 accgtattgt gcagttcttt gcccagcgtc cgcaggtaca agaacgtctg acgcagcaaa
     6421 ttctcattgc gctacaaacg ctgctgggca ccaataatgt ggctgtctct atcgacgcag
     6481 tgcattactg cgtgaaggcg cgtggcatcc gcgatgcaac cagtgccacg acaacgacct
     6541 ctcttggtgg attgttcaaa tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc
     6601 gtcatcacaa ctgataaaaa ggcaggaacc gtggagcgca acgtcacgct cgattttgtt
     6661 cgcggcgtcg ccgttctggg gatcctgcta ttaaacatca gcgcctttgg gctaccaaag
     6721 gcggcttatc tcaatcccgc ctggtacggc gctattacgc cacaggatgc atggacctgg
     6781 gcatttctcg atctcatcgg ccaggtgaaa ttcctcacgc tttttgcgct gttgtttggt
     6841 gcgggcctgc aaatgttgct gccccgtggc agacgatgga tccagtcgcg gttaacgctg
     6901 ttagtcttgc tgggctttat tcacggttta ttgttctggg acggcgatat tctgctggct
     6961 tacgggctgg tgggcttaat ctgctggcgg ttggtgcgcg atgcgccatc ggtaaaaagc
     7021 ctgtttaata ccggcgtcat gctttatctg gtggggcttg gcgttttgct gttattgggg
     7081 ttgatttccg atagccagac cagccgcgcc tggacacctg atgcatcggc tattttgtat
     7141 gaaaagcact ggaaacttca cagtggtgtt gaagcgatca gtaatcgtgc cgatggtgtt
     7201 ggcaacagtt tactggcact gggcgcacag tatggctggc aactggcagg gatgatgctc
     7261 attggtgccg cattgatgcg cagtggctgg ctgaaagggc agttcagctt acgtcactat
     7321 cgtcgtactg gttttgtgct ggtggcgatt ggggtgatca ttaaccttcc tgccatcgcc
     7381 ctgcaatggc gactggactg ggcgtatcgt tggtgcgcct tcttacttca gatgccgcgg
     7441 gaactgagtg cgccgtttca ggcgattggc tatgcgtcgc tgttttatgg cttctggccg
     7501 caattaagcc gctttaagct ggtgctggca atcgcctgtg tcggacggat ggcactgacc
     7561 aactatctat tgcaaacgct gatttgtact acgctttttt accacctcgg tttgtttatg
     7621 cagtttgacc gtctggagct gctggcgttt gttattccgg tatggctggc gaatatcttc
     7681 ttctctgtta tctggctgcg ttacttccgc caggggccgg tggaatggct ctggcgtcag
     7741 ttaactttgc gtgctgccgg accgacaata tctaaaacat caagataacg atctggatca
     7801 cattcgttaa caaaacggct gtaaccgttt ccattgctgt gactcgattc acgaagtcct
     7861 gtattcagtg ctgacaaaat agccgccagc aagcagtcat ttactgcaat ctcataacag
     7921 gtagtgaata tgatcaccat tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg
     7981 gtttcccggg tgctcaataa cagcacgctg gtcagtgccg acacgcgtga agcagtaatg
     8041 aaagctgtga gtgagctgga ttatcggcca aacgccaatg cccaggcgct ggcaactcag
     8101 gttagcgaca ccattggcgt ggtggtgatg gacgtttctg atgctttttt cggcgcgctg
     8161 gtaaaagcgg tggatctggt cgcccagcag catcagaaat acgtgctaat cggcaatagc
     8221 tatcatgaag cggaaaaaga gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat
     8281 gcgttgattg ttcactcaaa agcattgagt gacgatgaac tggcacaatt tatggataac
     8341 attcccggta tggtgttaat caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc
     8401 ctggataatc tcagcggtgc ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa
     8461 cgtattggtt atctttcttc cagccacggc attgaagatg acgccatgcg taaagcaggt
     8521 tggatgagtg cgttgaaaga gcaggatatt attccgccgg aaagctggat tggcactggc
     8581 acgccggaca tgccgggcgg tgaggcggcg atggttgaac tgctggggcg caatctacaa
     8641 ctaaccgctg tatttgctta taacgacaat atggccgctg gcgcactgac agcattaaaa
     8701 gataatggca ttgcgattcc gttacatctc tcaatcattg gtttcgatga tattcccatc
     8761 gcccgttaca ccgacccgca attaacgact gtgcgttatc ccattgcttc aatggctaaa
     8821 ttagctaccg aactggcctt gcagggggca gcagacaata ttgatcctcg tgccagccac
     8881 tgttttatgc cgacgttagt gcgtcgccat tctgtcgcaa cacgccagaa tgcggcggcg
     8941 atcactaact caacaaatca ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt
     9001 atcttaacaa tgtgatggct atgattgcac cgttttaacg ttgtaacccg tatgtaacag
     9061 tgaataatca ctttcaccga ggtaatagcg tcataacaac aattaaagcc gttttctgga
     9121 gcgttaccgg gcatggaaga acgaatttta aaaagtgagc ttcggcgttc agtaacactt
     9181 cattaactct actgccccgc cgagcattta tctcaagcac taccctgcat aagaaaaacc
     9241 ggagatacca tgaataagaa ggtgttaacc ctgtctgctg tgatggccag catgttattc
     9301 ggtgccgctg cacacgctgc tgatactcgc attggtgtaa caatctataa gtacgacgat
     9361 aactttatgt ctgtagtgcg caaggctatt gagcaagatg cgaaagccgc gccagatgtt
     9421 cagctgctga tgaatgattc tcagaatgac cagtccaagc agaacgatca gatcgacgta
     9481 ttgctggcga aaggggtgaa ggcactggca atcaacctgg ttgacccggc agctgcgggt
     9541 acggtgattg agaaagcgcg tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg
     9601 tctcgtaagg cgctggatag ctacgacaaa gcctactacg ttggcactga ctccaaagag
     9661 tccggcatta ttcagggcga tttgattgct aaacactggg cggcgaatca gggttgggat
     9721 ctgaacaaag acggtcagat tcaattcgta ctgctgaaag gtgagccggg gcatccggat
     9781 gcagaagcac gtaccactta cgtgattaaa gaattgaacg acaaaggcat taaaactgaa
     9841 cagttacagt tagataccgc tatgtgggat accgctcagg cgaaagataa gatggacgcc
     9901 tggctgtctg gcccgaacgc caacaaaatc gaggtggtta tcgccaacaa cgacgcgatg
     9961 gcaatgggcg cggtagaagc actgaaagca cacaacaagt ccagcattcc ggtgtttggc
    10021 gtcgatgcgc tgccagaagc gctggcgctg gtgaaatccg gtgcactggc gggcaccgta
    10081 ctgaacgatg ctaacaacca ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat
    10141 ggcaaaggtg cggctgatgg caccaactgg aaaatcgaca acaaagtggt ccgcgtacct
    10201 tatgttggcg tagataaaga caacctggct gaattcagca agaaataaga ctgattgggc
    10261 gcagtcgttt actgcgccct tattgtatga ccaacaaggt aaaattatat ggtcagctca
    10321 acgactccgt cttccgggga atacttgttg gaaatgagcg gtatcaacaa gtcttttcct
    10381 ggtgttaagg cacttgataa cgttaactta aaagtccggc cacattctat ccatgcatta
    10441 atgggggaaa atggtgcggg aaaatcaaca ttattaaaat gcctgtttgg tatttatcaa
    10501 aaagactccg gcaccatttt attccagggt aaagagatcg atttccattc cgccaaagaa
    10561 gcactggaaa atggtatttc gatggtacac caggagttaa atctggtatt acaacgttcg
    10621 gtgatggaca acatgtggct ggggcgatat cccaccaaag gcatgtttgt cgatcaggac
    10681 aaaatgtacc gcgaaaccaa agcgattttt gatgaactgg atattgatat cgatccgcgt
    10741 gcacgcgtcg gtacattatc tgtttcgcaa atgcagatga tcgaaattgc caaagcgttt
    10801 tcctataacg cgaaaattgt gattatggat gaaccgactt cttcgttaac cgaaaaagag
    10861 gtcaatcatc tgttcactat tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat
    10921 atctcgcata aaatggaaga aatcttccag ttatgtgatg aagttaccgt attgcgcgac
    10981 ggtcagtgga tcgctaccga accgctggca gggctgacga tggacaagat catcgccatg
    11041 atggttgggc gttctcttaa ccagcgtttc cctgacaaag aaaacaagcc gggcgaagtg
    11101 attctggaag tgcgtaacct gacgtcgctg cgccagccgt cgatacgcga tgtctcgttt
    11161 gatctgcata aaggggagat tctcggtata gccggtctgg tgggagcgaa acgtaccgat
    11221 attgttgaga cgttatttgg tattcgcgag aaatcggctg gcaccattac gttgcacggc
    11281 aaaaagatca ataaccataa tgccaacgaa gccataaacc acggatttgc actggtaact
    11341 gaagagcgcc gctcaacggg aatttatgcc tatctggata ttggctttaa ctcgttaatt
    11401 tccaatatcc gcaactataa aaataaagtt ggtttactgg ataactcgcg gatgaaaagc
    11461 gatacccagt gggtgattga ttcgatgcgg gtaaaaacac cggggcatcg gacgcaaatt
    11521 ggttcgctct ccggtggtaa tcagcaaaaa gtgattattg gtcgctggct gttaacgcaa
    11581 ccagaaatat taatgctcga tgaaccgacg cgcggtattg atgttggggc taagtttgag
    11641 atttatcagt taattgccga actggcgaag aaaggcaagg ggattattat tatctcctcc
    11701 gaaatgccag agttgttagg gataacagac cgtattctgg tcatgagcaa tggtctcgtt
    11761 tccggaattg tcgatacaaa aacaacaacg caaagcgaaa ttctgcgtct tgcgtctttg
    11821 cacctttaag attaggggct tcccatgagt gcgttaaata agaaaagttt tcttacttac
    11881 ctgaaagagg gcggtattta cgtcgttctt ttagttttgc tggcgattat tattttccag
    11941 gacccaacat ttttaagtct gttgaactta agtaatattc tcacccagtc atcggtgcgt
    12001 attattatcg cgcttggtgt ggcagggtta attgtcaccc aggggactga cctttccgca
    12061 ggtcgccagg tggggctggc ggcagtagta gcagcgacgt tattgcagtc catggataac
    12121 gccaacaaag tgttcccgga aatggcgacg atgccgattg cgctggttat tctgattgtc
    12181 tgtgtcattg gtgcggtgat cggtttgatc aacggcttga ttatcgctta tctcaacgtg
    12241 acgccgttca ttaccacgct cggtacgatg attatcgtct acggcattaa ctcgctctat
    12301 tacgactttg tcggggcgtc gccaatttcc ggttttgaca gtggcttctc tacctttgct
    12361 caaggctttg tcgcgctggg gagtttccgt ctctcttaca tcaccttcta cgcgttgatt
    12421 gcggtggtgt ttgtctgggt gttatggaac aaaacccgct tcggcaagaa catttttgcc
    12481 attggcggta acccggaagc ggcgaaagta tctggtgtca acgtcggcct gaacctgctg
    12541 atgatttacg cgttgtctgg tgtgttctat gccttcggcg ggatgttaga agccggacgt
    12601 atcggctcag ccaccaacaa cctcggcttt atgtatgagc tggatgctat cgcggcgtgc
    12661 gtggtaggcg gcgtatcgtt cagcggcggt gtggggacgg taattggtgt ggtgaccggg
    12721 gtaattattt ttaccgtcat taactatggc ctgacgtata tcggcgtaaa cccgtactgg
    12781 cagtacatca tcaaaggggc gattattatc ttcgccgtgg cgctggattc actgaaatac
    12841 gcgcgtaaga agtgatgtga ttgtggtccg cattcgttca taaacgcaca taacatcatg
    12901 atctgtgtgg ttttgtagtc cggataaggc gttcacgccg catccggcga caggagccaa
    12961 atgccggatg caacgctggc gcgt
//
LOCUS       DADSZD010000124        12960 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.125, whole genome shotgun sequence.
ACCESSION   DADSZD010000124 DADSZD010000000
VERSION     DADSZD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.125"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            159..395
                     /locus_tag="J1Z28_003257"
     CDS             159..395
                     /locus_tag="J1Z28_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006675846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major outer membrane lipoprotein"
                     /protein_id="HBA7718067.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(458..1462)
                     /gene="ldtE"
                     /locus_tag="J1Z28_003258"
     CDS             complement(458..1462)
                     /gene="ldtE"
                     /locus_tag="J1Z28_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBA7718068.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1611..2027)
                     /gene="sufE"
                     /locus_tag="J1Z28_003259"
     CDS             complement(1611..2027)
                     /gene="sufE"
                     /locus_tag="J1Z28_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBA7718069.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(2040..3260)
                     /gene="sufS"
                     /locus_tag="J1Z28_003260"
     CDS             complement(2040..3260)
                     /gene="sufS"
                     /locus_tag="J1Z28_003260"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBA7718070.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGSLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3257..4528)
                     /gene="sufD"
                     /locus_tag="J1Z28_003261"
     CDS             complement(3257..4528)
                     /gene="sufD"
                     /locus_tag="J1Z28_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBA7718071.1"
                     /translation="MAGLPNSSNALQQWNHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4503..5249)
                     /gene="sufC"
                     /locus_tag="J1Z28_003262"
     CDS             complement(4503..5249)
                     /gene="sufC"
                     /locus_tag="J1Z28_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBA7718072.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5259..6746)
                     /gene="sufB"
                     /locus_tag="J1Z28_003263"
     CDS             complement(5259..6746)
                     /gene="sufB"
                     /locus_tag="J1Z28_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBA7718073.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6755..7123)
                     /gene="sufA"
                     /locus_tag="J1Z28_003264"
     CDS             complement(6755..7123)
                     /gene="sufA"
                     /locus_tag="J1Z28_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBA7718074.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7672..7860)
                     /locus_tag="J1Z28_003265"
     CDS             complement(7672..7860)
                     /locus_tag="J1Z28_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718075.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(7960..8370)
                     /gene="menI"
                     /locus_tag="J1Z28_003266"
     CDS             complement(7960..8370)
                     /gene="menI"
                     /locus_tag="J1Z28_003266"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBA7718076.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8367..11423)
                     /locus_tag="J1Z28_003267"
     CDS             complement(8367..11423)
                     /locus_tag="J1Z28_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBA7718077.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTIIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALLGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11637..12749
                     /gene="ydiK"
                     /locus_tag="J1Z28_003268"
     CDS             11637..12749
                     /gene="ydiK"
                     /locus_tag="J1Z28_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBA7718078.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT         3048 a         3465 c         3172 g         3275 t
ORIGIN      
        1 tctcaatttt tttatctaaa acccagcgtt cgatgcttct ttgagcgaac gatcaaaaat
       61 aagtgccttc ccatcaaaaa aatattctca acataaaaaa ctttgtgtaa tacttgtaac
      121 gctacatgga gattaactca atctagaggg tattaataat gaaagctact aaactggtac
      181 tgggcgcggt aatcctgggt tctactctgc tggcaggttg ctccagcaac gctaaaatcg
      241 atcagctgtc ttctgacgtt cagactctga acgctaaagt tgaccagctg agcaacgacg
      301 tgaacgcaat gcgttccgac gttcaggctg ctaaagatga cgcagctcgt gctaaccagc
      361 gtctggacaa catggctact aaataccgca agtaatagta cctgtgaagt gaaaaatggc
      421 gcacgatgtg cgccattttt ttgcctgcta tttaccgcta ctgcgtcgcg cgtaacatat
      481 tcccttgctc tggttcccca ttctgcgctg actctactga aggcgcattg ctggctgcgg
      541 gagttgctcc actgctcacc gcaaccggat accctgcccg acgatacaac gctttatcga
      601 ctaacttctg atctacagcc ttattgtctt taaattgcgt aaagcctgct ggcagcgtgt
      661 acggcattgt ctgaacgttc tgctgttctt ctgccgatag tggtcgatgt acttcaacat
      721 aacgcatccc gttaggttcc acggaatatt tcaccggttc gttgatcact ttcaccggcg
      781 ttcccgtccg cacgctggag aataaagctt taatatccgg tgcattcatg cgaatacagc
      841 ccgaactgac gcgcaaaccg acgctgtccg gcgcactggt gccatgaatg aggtattcgc
      901 cattaccatg cgcgaggcgc agtgcgtaac gtcctagtgg gttattgggc ccggcaggaa
      961 cgactggtgg taatttaatg ccacgctcca gcgaacgttg acgaatgccc gccgtaggcg
     1021 tccaggttgg gttagggatt ttctgcccca cacgcgtttc catcaccggc gtttccagcc
     1081 cctgcaaacc aatacctatc ggataaacct gcacgatatt ttctcccggc ggataataat
     1141 aaaggcgcag ctctgcaagg tttacaataa tcccctgacg cggcgcatca ggtaataata
     1201 gttgtgaagg aatagttatc gtcgtaccgg gttttggcac cggggcgata gtgttattgg
     1261 cttcaaggat caacattgcc gcagtatcaa aacgtcgggc gatggcctga aggtttttat
     1321 ccccttcttg caccgtatac gtttgatttt gcccaaccag tcgactgccg gttggtggta
     1381 gcggataatc aaccgcccag gcagcctgga tggcgctaaa agcgccgata agcgtgagtg
     1441 taagcaaaga cgcgcgtttc attgtaaacc tcctgtattt gccggagact cacgctgaaa
     1501 cgtcggatgg cgcttatgtt cacctgaaac caaaacactc ctgtgcaggc cagtgtaaac
     1561 attgaccacc cggcaatgtg agccaaccgg atgaaagctg tccctttagt ttagctaagt
     1621 gcagcggctt tggcgcgaat tgcgcgaatc atcgcttcca gaccttgtga gcgtgatggg
     1681 gtgagatgtt gggtgagcgc cattttttca aaccacgggc gcacatcgaa attgacaata
     1741 tcctgcggcg tcatttgatc gtaaagaata aagacgaccg caataagccc ttttacaatg
     1801 gccgcatcgc tgtcgccctg taattcaata attccctgcg cattttgacg catcacaatc
     1861 cacacctgac tctggcagcc ctggatgcta ttttgcgggc ttttgtcttc gtcacgtaat
     1921 tctggcagac gctgacccaa ctcaataatg tagagatatt tctcttccca gttggcgcag
     1981 cgtaaaaaat tacgcagcaa tttttcttta tccggcaata aagccatagt gcctccctgt
     2041 tatcccagca gacggtgaat acgttgcagg ccggtcacca gacgatccac ttcttcatgg
     2101 gtgttataca tggccagcga tgcccgacac atcgcaggga cgttgtaata ggccattaat
     2161 ggcattgcgc agtgatgtcc ggtacgcaca gcaatgccgt aattatcgag aaaactgcca
     2221 acatcatagg cgtggtgttt accgagatta aaagcaataa cgccaagcct gttttgtggg
     2281 ccatagagag tgagatccgg tacagattcc aactgtgata acgcgtaatg catcagattc
     2341 tgttcatact cggctatgtt attaagcccc agcgccgaaa catactccag cgccgcgcca
     2401 agaccaatga tgcctccggt attgggtgta ccggcttcaa accgccatgg tgctttggtc
     2461 caggtagtgc cttcactcag gctgacggtg gcgatcatag aaccgccccc ttcccacggc
     2521 ggcatctcct gcaacaaggc ttctttgaca taaagaatgc caattccggt agggccatac
     2581 agtttatgcc cggagaacac gtaaaaatcg caatccagcg cctgaacatc caccggatga
     2641 tgcatcaccg cctgagcgcc atccaccagc acttttgcgc catgttgatg cgcaagtgtg
     2701 atcatctccg ccagcggatt ttctgtccca agcacgttgg agacatgagt aattgccagc
     2761 agacgagttt tctcatcaaa cagcgtaggc agcgtctcca gttgcagaga accgtcgggg
     2821 ttgagcggga tcacacgcag ctctgcgcca acgcgtgcac aaagcatctg ccaggggaca
     2881 atgttagcgt ggtgctccat ctgactgatg atgatgttat cgcccgctcg cacattgctg
     2941 ttgccccagc tattggcgac cagattgatc ccttccgtcg tgccgcggac gaacaccagc
     3001 tcttccgccg aacgggcatt aataaatagc gaagcacgct tgcgcacgtt ctccattttc
     3061 tcggtcgcct gggcgcttaa ggtatgaata ccgcgatgca ccgccgcgta gccatgacga
     3121 taaaactcag cctcggcatc aatcacctgg ctcggtttct gcgcactggc ggcgctgtcg
     3181 agataagcca gcggcaaacc gtttacctca cgagaaagca ccggaaagtc ggcccgcact
     3241 ttgtcgacgg aaaaagtcat cttgcacctc ctggcagtcg ttgaccgatt cgggccagca
     3301 cctgctgttt aagcccctca tcacacagtg cttccgtcag ttcggcagca aaggcgtaga
     3361 tgatcatctg ctgggcatct tgctgattga tcccgcgcga gcgcagataa aacatctgtt
     3421 cgtcatcaat acgccccacc gtcgcgccgt ggctgcattt cacatcatct gcatagattt
     3481 ccagctgcgg tttcgtatcc acttccgcca gtttgcccat cagcagatta ttgttggtca
     3541 tctgaccatc cgttttgatg gcgtgctgcg cgacgttgat caaaccgtta aataccgcgc
     3601 ggcctttgtc actgacgata gttttgtgca actgtcggct attacaaaaa cctttattgt
     3661 gttccagcca ggtacgagta tcacacacct cgtttttcac cggcatcgcc aggctattga
     3721 tccgcagcgt gctgttttcg ccattgagtt gcgtactggt gttgtgtcgt aacactgcgc
     3781 cacccagcag gaaactgtgg ctaaatgcgg tggcatcgtc agccagcaac aaatcgttat
     3841 gagcaaagtg gtgactgagc gggttttcaa acgccagctt gatatgctgc aaatgggcgt
     3901 tcgctgcgac gttgatagtg aaccgtgcgc cggtgaagtg acgagcatca ttaaggctga
     3961 caaaatgttc gatcaccgtt gcttcggcac cttccgccag atccagatga tgccggtaat
     4021 gggcagtatt cacctcttca cctgccacgc cctgggtgat atgcattaac agcaatggct
     4081 ttgccggccg ttgaccgcgc ttcacggcga tatgcgtcac gctttgtgcc aggctttccg
     4141 tcaaatgcag aaacacttcc gcctgaatag cgtcgggtaa cccctgacgg tcgtcgttaa
     4201 tgctcacttc atatccgctg ccttcagttg catcgctcag tgcgggcacg taacgtccat
     4261 cgacaaacac cagccgcacg gcgtctaatg ttaacgctaa ggcatcacgc tgctgtgggg
     4321 atatctcccc cgcaatgctg acaaactggc tattggtcag cccgtccagc ggcgtatatt
     4381 tccagttttc atgtttacgt gtcggcagtc cggtacgcag caattgttgt aaatgctgct
     4441 gtgcttgcgg ggagcgtttc gccccttcag cttcaaacaa atgattccac tgttgcagtg
     4501 cgttactgct gttcggtaag ccagccataa ccctgctcct ccagttgttt gaccaacgtg
     4561 aaatcgccgg atttcacaat tcgtccctga tacagcacat ggacgtaatc aggcttgatg
     4621 tagtcgagaa tgcgttggta gtgcgtaaca atgatgaatg aacgcttgcc gtcacgcaga
     4681 gagttcacgc catcggcaac cacttttaat gcgtcaatat ccagcccgga gtctgactca
     4741 tcaagaatgc ataactccgg ttccagcacc gccatttgca aaatatcgtt gcgctttttc
     4801 tcgccgccgg aaaaaccaac gtttaccgaa cgggttaata aatcttccgg catcttcagg
     4861 agagcgattt tctcttccat caaatcctga aaatcaaagc ggtcgagcgt ttcctggccg
     4921 cgatagctgc gcaccgcatt aagtgccgtt tgcaggaaaa actggttact gacgccggga
     4981 atttccaccg gatactggaa ggccataaag atgccttcac ctgcacgatc ttccggcgac
     5041 agcgcaagca aatctttgcc tttaaactca accgtgccgc tcgtcacttc ataatcttct
     5101 cgcccggcaa gcgttgccga taaggtactt ttgcccgaac cattgggccc cataatggcg
     5161 tgaacttcgc cgggacgaac gtcgaggctt aatccacgca ggatagcttt atcttccacg
     5221 ctgacgtgta aatctttaat acttaacatg tttattcctt atccgacgct gtgttcaaga
     5281 ctgatggcga ggagtttttg tgcttcaacg gcaaattcca gcggcagttc cgagaacacg
     5341 tctttgcaaa aaccgttaac aatcatcgag atggcatctt cttcgctgat cccgcgttgc
     5401 aggcagtaaa acagttgatc ttcaccaata cgtgatgtcg ttgcctcgtg ctccagctga
     5461 gcgctgttat tgcgacattc gacatacggg aaggtatgcg ccccacaatt agcgccaatc
     5521 agcattgagt cgcactgagt gaaattgcgc gcattggttg ccgtcggcat gattttcact
     5581 aagccgcgat aactgttctg actatgtccg gcagagatcc ctttcgatat aatggtcgat
     5641 ttggtgtttt taccgatgtg gatcatcttg gtgccggtat ccgcttgctg atgaccgctg
     5701 gtcagagcca ctgagtaaaa ctcaccaatg gagttatcgc cgcgcaaaat gcagctggga
     5761 tatttccacg taatcgctga gccggtttct gattgcgtcc atgacatttt gctgttttcg
     5821 ccttcgcaca aagcacgctt ggtgacgaag ttgagaatac cgccggtgtt gttatcgcca
     5881 gggaaccagt tttgtaccgt ggaatatttc acctcggcgt ttttatggat gatgacttcc
     5941 accaccgccg cgtgtaactg atagctgtca cgcaccggag cggaacagcc ttcaatgtag
     6001 ctaacgtagc tgtcttcgtc ggccaccaga atggtgcgct caaactgccc ggttttttcc
     6061 gcgttaatgc gaaaataggt ggaaagttcc atcgggcaac gcacgccttt aggcacataa
     6121 ataaacgtac cgtcagaggc taccgccgca ttaagtgcgg caaagaagtt gtcattcccc
     6181 ggcaccacgg tgccgagata tttacgcacc agttccgggt gatcgtggat cgcctcgcca
     6241 aaggaacaaa aaataattcc ctgctccgcc agtttttcgc gataagtagt ggcaaccgaa
     6301 acagagtcga aaatggcatc cactgccacc tctttgcctt cccgcacggg aacgcccaac
     6361 tgctcaaacg ccgcctccac ctctttactt aaaaaggcgt tcgcgccagt ttgctgcacc
     6421 gcgccgggtt cagacgcgca gttgtcgtca caattaccgc acgatggtgc cgagtagtag
     6481 ctgtaatcct gataattcag cttgtcgtag tgcgctttca accagtgcgg ttcttccatc
     6541 tccagccatg cgtgataggc gttgagacga aactccagca tccactccgg ctcattacgc
     6601 ttcgccgaaa ttgcgcgcac cacctcttcg tttatcccct ttgccagctc atcggtggct
     6661 aactgggtga agaatccttc tttataattc agcgggccgc cggtccaggt tttgacatcg
     6721 tcagttgctt cagtattacg agacatagta ccgcctatac cccaaagctt tcgccacagc
     6781 cacattcatt ctgggcttta gggttgtgaa atttgaatat ctgattaagt ccttcacgaa
     6841 cgaaatcgac ttccgtgcca tcaataaaag gcatcgcttg cagcgggaca aacagcttcg
     6901 cgccgtcgtg ttcaaacagc agatcgtctt tgtccggctc gctaacactg tcgagcacat
     6961 agccaaagcc cgcgcagccc gtttgcttaa cgcctaagcg cacgccgatc ataccaggct
     7021 gctttgccac cagttcgcgg atgtgtatcg ccgctgcggg tgtcagcgtt aagccttgcc
     7081 aggcgaaatc ctgtggatta aaggttcctg aatgcatgtc catcgattta cctcacttca
     7141 tcgctttcag cgtataacag catgttagtg ataatgatta tcagttcaac ccagcaaacg
     7201 caggggcttt agcgtaaaac atgcttttta cctgctttta ataagcatag accctgatgt
     7261 gtgggttaac aggcacgcta agaattaggt atctcattgt tagataatga ttattatcta
     7321 aggagcatta aggctgtgat gaaaaagaaa aagttgtatt gaaaatgact atttaagaga
     7381 taggtaaaaa agtgcagcgt tcagaaataa gaaaaccctt aagtctgtgc gacacaggct
     7441 taagggtttc taccccatcc ggcgcttatc tccggcactc tcagtggctt agctcttgaa
     7501 ggggcgataa ggataatctc ataaagctaa cccactgttt taacacaaac tgcgatttgt
     7561 attatttttg aacgttatca ggcgggtaga taggctgtat taaaaaggcc ctcgaacctg
     7621 gacgaagatt aatccaggcc gatttggcga actcgcgaca ttaaatcagg atcaatgcac
     7681 gcccagacgc caggcgaagt agatttcttc ttttaattca gcagaagaga gagtaagcag
     7741 gtcagcaaat tcaagttcta attgtttcag acgcttgaga tattgggcag gtgaaagatt
     7801 gctttggtca cggcgtaaaa attcaatggc cagttgggtg ggatcaagtt gagtagacat
     7861 agcatcctcg cttttagaca agacctgcac agtataccac cgtttactgt gcagataata
     7921 accaaaagca atatgcgtca cacttttctg gtgacaacgt cacaaaatag cggtcgtcaa
     7981 tcgtgacgaa cagcacaaac gccctttctc atcgaagatt tcaatctgcc agacctggtg
     8041 acgcgaaccg agatgcaacg gtttgcatac gccgcgcacc cgcccttctc gtgccgagcg
     8101 gacgtggtta gcattgattt ccagaccaac cactttttgc tcaccttcgg tacataaata
     8161 accggcaacg gaaccgatac tttcggccag taccacggat gcacctccat gcagcaaccc
     8221 gaaaggctgc tttgtccgtg agtctactgg cattgtcgct tcaagggtgt catcaccaat
     8281 atgttcaaag cgaatatcca gcaatcccac catgtttcct tcccccatag cattcagtgc
     8341 ttccagggtg atttttcgtt tccatatcat ttaataatct ccagtaaagc ctgcacagga
     8401 tggcgtaccc ctgtgccttc aacccgtttt acctgactac ggcaggaata tccggtcgcc
     8461 aggcagcggt tacgcggcag tcgctgcata gcctgatgcc aggataattc atagatcccg
     8521 agcgaatttt tatggttttt cgcttcatgt ccgtaagtcc ctgccatgcc gcagcacccc
     8581 acgctgacat tttccagttt cgcgccaaaa cgggcaaata ttgccgccca ttgtgctggc
     8641 gcgcctagca aagcggtaac ttcggtacag tgaccaaaga aataccatga ttcaccgctg
     8701 actgtagcca ctggctgtga ctcaagtgcg cttgccagcc attcatttgc cagtaagacg
     8761 ttaaactcgc cacgctcctc gcccagagcc agtttatatt catcgcgata acaaagtacc
     8821 agcgccgggt cgacgcctac cattggcata cccagcttcg ccatacggtt gaggaaatcc
     8881 gccgtctttt tcgccgtctt cgcaaaacga ttaagaaaac ctttgatatg ctgggctttt
     8941 ccatttggcg aaaatggcag taacacgggc tggaaaccta atttttcgac cagacggaca
     9001 aaatccgcca ccacttgagc atcgtaatag ctggtaaagg ggtcctgcac caccagcacc
     9061 gtgcgcgctt tctgctctgc attgagagct tcaagctgtt ccagcgtcat gtttgccgag
     9121 cgatgcccca ccatttgttg ttgtagcgag gggaccgaca gcagcggcaa atcaaccatg
     9181 ccgatatgtt tttccgagag tttgcgcacc agcggctggt taatgaagaa gttaaaggtt
     9241 ttcggcgcgc gtgccatcag cggtgcgtaa ctctcaaccg tagcgacgag gtggtcgcgc
     9301 agcgggcgta aataacgggt gtgatagagt tgcaggaaac gagagcgaaa ctccggcaca
     9361 tcaattttga tggggcactg agtcgaacaa gctttacagg ccagacagcc cgacatcgcc
     9421 tctttgactt cgtgtgagaa gtcatattcg cctttattcg catgccaact attgcgcgtg
     9481 cgggcaatta acgtccgcaa actgacgccg gattcaggca gttcttgttc cagtttgagt
     9541 gggtcaacgc cgcgatccgc caacaaacgc agccattcac gtaccagcgt tgcgcgccct
     9601 ttcggtgaat gaatccggtt ctgggtgatc ttcatcgacg gacacatcgg actacgagca
     9661 tcaaagttga agcataaacc gttgccgtta cactccattg caccacgcca ctgctggcgt
     9721 accgcaatgg ggatctgtcg atcgaatgta ccgcgcttca ccgcgtccac tttcatcatc
     9781 ggcgcatcga gaccttctgg cgggcaaatc ttcccggggt tgagtcggtt atgcgggtca
     9841 aatgccgctt tcactttacg cagttctgca aaaagttcct caccgaaaaa cgccgggctg
     9901 tattcggcgc gaaaaccttt gccgtgttcg ccccacaaca aaccaccgta tttcgcggta
     9961 agcgccacta catcatcaga gatttgcttc atcaaaatct cttgttgcgg atcgcacata
    10021 tccagtgccg gacggacgtg caagacacct gcgtcgacgt gaccgaacat accgtagctt
    10081 aagccgtggc tgtcgagcag cgcgcgaaat tcagcaatat aatccgccag gtgttccggc
    10141 ggtacgcagg tatcctcagc aaacggaatt ggcttagcag cacctttggc attgccaagc
    10201 agaccaacgg cttttttgcg catcgcatag atacgttcaa cacccgccag ctcgcggcac
    10261 acctgccagc cgatcactcc tgcctgttgg cttgcaatca gctcatcaag ccgcacacag
    10321 agtgcattaa ctcgctcgtc aatcagtgcc tcatcatcac cggcaaattc cacaatgttc
    10381 agcccgagca tctctttatc aggcacatcg gtaatcaact cgctgacgga atgccagaca
    10441 atatcttccc gcgccagatt cagcactttt gagtccaccg tctctaccga aagcgcccgc
    10501 gcctcaacca taaacggcgc gttacgcagc gcggagtcaa aagagtcata tttgacgttc
    10561 accagacggc gtactttagg caagcgcgta atatccagtc gcgcttcggt aataaaggcc
    10621 agcgtccctt ctgaacccgt cagaatgcgc gtcaggtcga actcggtcat ctcatcgtta
    10681 aagacatgac gcagatcgta accggtaaga aagcggttaa gcttggggaa gttgtcgatg
    10741 attaactggc gttgctgacg gcaacgttga taaaccgtgt tataaattcg cccgattatg
    10801 gtattggatt tacccagcgt ttccgccagt tcgacgggta aaggttgcgt atcgagaata
    10861 tcgcccccca acaacaccgc gcgtacgcca agtacgtgat ctgacgtttt gccatagacc
    10921 agcgatccct gaccggatgc atcggtattg atcatcccgc cgagcgttgc ccggttgctg
    10981 gtcgaaagtt ctggcgcaaa aaagtagcca aagggtttca ggtactgatt gagttggtct
    11041 ttaatcaccc cggcctcaac gcgcacccag ccctcttcag ggttaatttc gatgatgcgg
    11101 ttcatatggc gggacatatc aacaataatc ccctggttga gcgcctgacc gttagtgccg
    11161 gtgccgccgc cgcggggggt aaagatcaac gatgaatagc gttcctgcgc ggcaagacgg
    11221 gcgatcagcg ctacatctgc ggttgaacgc ggaaatacca ccgcatcggg gagaagttgg
    11281 taaatactgt tgtcagtcga cattgtcaga cgatcggcat aacttgtcgc cgtatcgccg
    11341 gtaaaaccct gttgctccag ctcttgcaaa aaattaagca ccagttgaac gacgccgggt
    11401 gcctgggaaa tctgtggaat cattatattg accctttcct gcggtctgtg atgagagatt
    11461 aatcgtttgc gtgttgcctg agttgttgta ccacattttt ttctaacacg cccatcagaa
    11521 ttaagggcag aatcgacctg ttaaaaaccg ctgaaattgc tcatcattat gcaggtgagt
    11581 ttcgcgtgtt cacgtcgcgt cgacgatttg acgcacaaaa aaggtgaaaa gtagttatgg
    11641 taaatgttcg tcagcccagg gatgtcgcac aaattctgct ttcggtgctg tttttagcca
    11701 tcatgattgt ggcatgtctg tggattgtac aaccctttat tctcggcttt gcatgggccg
    11761 gtacagtggt tatcgccacc tggccggtat tgttacgttt gcaaaagatc atgtttggtc
    11821 gccgctctct cgccgttctg gtgatgacgc tgttattagt gatggtgttt atcatcccta
    11881 ttgctttgct ggttaacagt atcgtcgacg gcagcggccc gctaattaaa gccatttcca
    11941 gtggtgacat gacgttaccc gatctggcgt ggcttaatac cattccggtg attggcgcga
    12001 agctatatgc aggctggcac aatttgctgg atatgggggg gacggcgatc atggcgaaag
    12061 tccgtcctta tattggcacc accaccacct ggttcgttgg gcaggcggcg catatcgggc
    12121 gctttatggt gcattgtgca ctgatgcttc tcttcagcgc cctgctgtac tggcgcggtg
    12181 aacaggtggc acaaggcatt cgccattttg caacccgtct ggcaggcgtt cgcggagatg
    12241 ccgccgtgct gctggcggca caggctatcc gcgcggtggc gctgggtgtg gtggtgacgg
    12301 cgttagtaca ggcagtgctt ggcggtatcg gcctcgccgt atccggcgta ccttatgcaa
    12361 ctttgctaac ggtgttaatg atcctctcct gccttgtcca gcttggaccg ttgccggtac
    12421 tgattccggc gattatctgg ctctactgga ctggcgatac cacctgggga acggtattgt
    12481 tagtttggag cggtgtggtt ggcacgctcg ataacgtcat ccgcccaatg ttaattcgca
    12541 tgggtgccga tttaccgctg atcctgatcc tctctggcgt tattggtggt ttgattgctt
    12601 tcgggatgat cggtctgttt attggtccgg ttctgttagc cgtttcctgg cgtctgtttg
    12661 ccgcgtgggt ggaagaagtc ccaccgccga cggaccaacc ggaagaaatt ctcgaagaac
    12721 ttggcgaaat cgagaaaccg aataagtaat ttctcatcag gcggctctgc cgcctgattg
    12781 ttaacccctg ctaattgata tttgcttgct cttccccata ttttcctgct taccgctatt
    12841 cagctggtag tacctgtcgc aaattcttta cagtttttaa actaatgaga cgaatctgat
    12901 cgacgcaaaa agtccgtatg cctactatta gctcacggtt ataaatcaac atattgattt
//
LOCUS       DADSZD010000125        12904 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.126, whole genome shotgun sequence.
ACCESSION   DADSZD010000125 DADSZD010000000
VERSION     DADSZD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.126"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1134
                     /gene="proV"
                     /locus_tag="J1Z28_003269"
     CDS             <1..1134
                     /gene="proV"
                     /locus_tag="J1Z28_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBA7718079.1"
                     /translation="YIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGK
                     STMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLD
                     NTAFGMELAGINAEERREKALDALRQVGLENYALSYPDELSGGMRQRVGLARALAINP
                     DILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNG
                     EVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSA
                     LKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALTQQQGLDAALIDAPLAVDAQTPLS
                     ELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1127..2191
                     /gene="proW"
                     /locus_tag="J1Z28_003270"
     CDS             1127..2191
                     /gene="proW"
                     /locus_tag="J1Z28_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBA7718080.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2248..3240
                     /gene="proX"
                     /locus_tag="J1Z28_003271"
     CDS             2248..3240
                     /gene="proX"
                     /locus_tag="J1Z28_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBA7718081.1"
                     /translation="MRHSVLFATAFATLISIQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3432..4616
                     /locus_tag="J1Z28_003272"
     CDS             3432..4616
                     /locus_tag="J1Z28_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718082.1"
                     /translation="MTKPNHEISPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4740..5477
                     /locus_tag="J1Z28_003273"
     CDS             4740..5477
                     /locus_tag="J1Z28_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBA7718083.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5467..5802
                     /gene="ygaH"
                     /locus_tag="J1Z28_003274"
     CDS             5467..5802
                     /gene="ygaH"
                     /locus_tag="J1Z28_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBA7718084.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(5821..5922)
                     /locus_tag="J1Z28_003275"
     CDS             complement(5821..5922)
                     /locus_tag="J1Z28_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718085.1"
                     /translation="MFNGRKRTIHLGMTSLISADIVTTVTNKVNVLF"
     gene            5893..6423
                     /gene="emrR"
                     /locus_tag="J1Z28_003276"
     CDS             5893..6423
                     /gene="emrR"
                     /locus_tag="J1Z28_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBA7718086.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6550..7722
                     /gene="emrA"
                     /locus_tag="J1Z28_003277"
     CDS             6550..7722
                     /gene="emrA"
                     /locus_tag="J1Z28_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBA7718087.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7739..9277
                     /gene="emrB"
                     /locus_tag="J1Z28_003278"
     CDS             7739..9277
                     /gene="emrB"
                     /locus_tag="J1Z28_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBA7718088.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9341..9856)
                     /gene="luxS"
                     /locus_tag="J1Z28_003279"
     CDS             complement(9341..9856)
                     /gene="luxS"
                     /locus_tag="J1Z28_003279"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBA7718089.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10006..11562)
                     /gene="gshA"
                     /locus_tag="J1Z28_003280"
     CDS             complement(10006..11562)
                     /gene="gshA"
                     /locus_tag="J1Z28_003280"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBA7718090.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(11635..12063)
                     /locus_tag="J1Z28_003281"
     CDS             complement(11635..12063)
                     /locus_tag="J1Z28_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7718091.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRNGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12060..12626)
                     /gene="yqaB"
                     /locus_tag="J1Z28_003282"
     CDS             complement(12060..12626)
                     /gene="yqaB"
                     /locus_tag="J1Z28_003282"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBA7718092.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         2968 a         3419 c         3338 g         3179 t
ORIGIN      
        1 tatatcgaac aaggactttc aaaagaacaa attctggaaa aaactgggct atcgcttggc
       61 gtaaaagacg ccagtctggc cattgaagaa ggcgagatat ttgtcatcat gggattatcc
      121 ggttcgggta aatccacaat ggtacgcctt ctcaatcgcc tgattgaacc cacccgcggg
      181 caagtgctga ttgatggtgt ggatattgcc aaaatatccg acgccgaact ccgtgaggtg
      241 cgcagaaaaa agattgcgat ggtcttccag tcctttgcct taatgccgca tatgaccgtg
      301 ctggacaata ctgcgttcgg tatggaactg gccggaatta atgccgaaga acgccgggaa
      361 aaggcccttg atgcactgcg tcaggtcggg ctggaaaatt atgccctcag ctacccggat
      421 gaactctctg gcgggatgcg tcaacgagtg ggattagccc gcgcgttagc gattaatccg
      481 gatatattat taatggacga agccttctcg gcgctcgatc cattaattcg caccgagatg
      541 caggatgagc tggtaaaatt acaggcgaaa catcagcgca ccattgtctt tatttcccac
      601 gatctcgatg aagctatgcg tattggtgac cgaattgcca ttatgcaaaa tggtgaagtg
      661 gtgcaggttg gtacaccgga tgaaattctt aataatccgg cgaatgatta tgtccgaacc
      721 ttcttccgtg gcgtcgatat tagccaggtg ttcagtgcga aagatattgc ccgtcgtaca
      781 ccgaacggct taattcgtaa aacccccggc ttcggcccgc gttcggcact gaaattattg
      841 caggatgaag atcgcgaata tggctacgtc atcgaacgcg gtaataagtt tgtcggcgct
      901 gtgtctatcg attcgcttaa agccgcgtta acgcagcagc aaggtcttga tgcggcgctg
      961 attgatgcgc cgttagcggt cgatgcacaa acgcctctta gcgagttgct ctctcatgtc
     1021 ggacaggccc cctgtgcggt gcccgtggtc gacgaggacc aacagtatgt cggcatcatt
     1081 tcgaaaggaa tgctgctgcg cgctttagat cgtgaggggg taaataatgg ctgatcaaaa
     1141 taatccgtgg gataccacgc cagcggcgga cagtgctgca caatccgcag acgcctgggg
     1201 tacaccggcg actgcaccga ccgacggcgg tggcgctgac tggctgacca gtacgcctgc
     1261 gccaaacgtc gagcatttta atattctcga tccgttccat aaaacgctga tcccgctcga
     1321 cagttgggtc actgaaggga tcgactgggt cgttacccat tttcgtcccg tctttcaggg
     1381 cgtgcgcgtt ccggttgatt acatcctcaa cggtttccag caattgctgc tgggtatgcc
     1441 cgcgccggtg gcgattatcg ttttcgctct catcgcctgg cagatttccg gggtcggaat
     1501 gggcgtggcg acgctggttt cgctgattgc catcggcgca atcggtgcct ggtcacaggc
     1561 aatggttacc ctggcgctgg tgttaacagc cctgctgttc tgtatcgtca taggtttgcc
     1621 gttggggatc tggctggcga gaagtccgcg agcggcgaaa attattcgtc cactgcttga
     1681 tgccatgcag accacgcccg cgtttgttta tctggtgcca atcgtcatgc tgtttggtat
     1741 cggcaacgtg ccgggcgtgg tggtgacgat catctttgcg ctgccgccga ttatccgtct
     1801 gacgattctg ggaattaacc aggttccggc ggatctgatt gaagcctcgc gctcattcgg
     1861 tgccagcccg cgccagatgc tgttcaaagt tcagttaccg ctggcgatgc caaccattat
     1921 ggcgggcgtt aaccagacgc tgatgctggc cctttctatg gtggtcatcg cctcgatgat
     1981 tgccgtcggc gggctgggtc agatggtact tcgcggtatc ggtcgtctgg atatggggct
     2041 tgccaccgtt ggcggcgtcg ggattgtgat cctcgccatt atcctcgacc gcctgacgca
     2101 ggccgttggg cgcgactcac gcagtcgcgg caatcgtcgc tggtacacca ctggccctgt
     2161 cggtctgctg acccgcccat tcattaagta actctgcact tgcccggtga cgccgggcat
     2221 atcaccctgc caaaaaaagg aataacaatg cgacatagcg tactttttgc gacagcgttt
     2281 gccacgctta tctctataca aacttttgct gccgacctgc cgggcaaagg cattactgtt
     2341 aatccggttc agagcaccat caccgaagaa actttccaga cactgctggt cagccgcgcg
     2401 ctggagaaat taggttatac cgtcaataaa cccagcgaag tggattacaa cgttggctac
     2461 acctcgcttg cttccggcga tgcaaccttc accgcagtga actggacgcc actgcatgac
     2521 aacatgtacg aagctgccgg tggcgataag aaattttatc gtgaaggagt atttgttaac
     2581 ggcgcggcac agggttacct gatcgataag aaaaccgccg accagtataa aattaccaac
     2641 atcgcgcaac ttaaagatcc gaagatcgcc aaactgttcg ataccaacgg cgacggtaaa
     2701 gcggatttaa ccggctgtaa cccaggctgg ggctgcgaag gtgcgatcaa ccaccagctt
     2761 gccgcgtatg gactgaccaa taccgtgacg cataatcagg ggaactacgc agcgatgatg
     2821 gctgacacca tcagtcgtta caaagagggt aagccggtgt tttactacac ctggacgccg
     2881 tactgggtga gtaacgaact gaaaccgggg aaagatgtgg tctggttgca ggtgccgttc
     2941 tccgcactgc cgggcgataa aaatgccgat accaaactgc cgaatggcgc gaattacggc
     3001 ttcccggtca gtaccatgca tatcgttgcc aacaaagcct gggccgagaa aaacccggca
     3061 gcagcgaaac tgtttgccat tatgcagtta ccagtggcag atattaacgc ccagaacgcc
     3121 attatgcatg acggcaaagc ctcagaaggc gatattcagg gccacgttga tggttggatc
     3181 aaagcccacc agcagcagtt cgatggctgg gtgaatgaag cgctggcagc gcagaagtaa
     3241 tttttattcg ggcggataag gcgtttttgc ctcatccgcc gctctgtaca caatgcctga
     3301 tgcgacgctg gcgcgtctta tcatacctac aaaccacacc gcacatttca acaatctatc
     3361 tttcatccca tattcatcaa catccgctat tattgatttc cagcttaatc atcacctgat
     3421 gaacaaaaat aatgactaaa cctaatcatg agattagccc ggcgctgatc gtgctgatgt
     3481 ctatcgccac cggtctggcg gtagccagta actattacgc ccagccattg ctcgacacca
     3541 tcgcgcgtaa cttttccctt tccgccagtt cggcaggctt tattgttacc gccgcgcagt
     3601 tgggctatgc cgcaggtctg ctgtttcttg ttcccctcgg tgatatgttt gaacgccgcc
     3661 gcctgattgt ctcgatgacc ttactggcag caggcggcat gttaatcact gccagcagtc
     3721 agtcgctggc gatgatgatc ctcggtacag cattaaccgg tttattttca gtcgtggcac
     3781 aaattctggt tccactggca gcgacgctgg cttcaccgga caaacgcggc aaagtggttg
     3841 gcaccattat gagcggtctg ctattgggga tcttactcgc acggaccgtt gccggattgc
     3901 tggcgaatct cggcggctgg cgcaccgtct tttgggttgc ttcgatgtta atggcactga
     3961 tggcgctggc attatggcgt ggtctgccac aaatgaaatc agaaacccac ctcaactacc
     4021 cacagttgtt gggttccgtt ttcagcatgt ttatcagcaa taagatcctg cgcacccgcg
     4081 cgttgctggg ctgcctgacc tttgccaact tcagcattct ctggacctca atggcctttt
     4141 tgcttgccgc tccacctttt aactacagcg atggtgtaat tggtctgttt gggcttgcgg
     4201 gagctgccgg agcgttgggc gctcgtccgg cgggcggttt tgccgataag ggcaaatcgc
     4261 accacaccac aactttcggt ctgctgctgc tgttactttc atggctggca atctggtttg
     4321 gtcacacttc cgtactggcg ctgattatcg gcatcctggt gctggacctc accgtgcagg
     4381 gcgtgcatat cactaaccag acggtaattt atcgaataca ccctgatgcg cgtaatcgcc
     4441 tgaccgcagg ttacatgacc agctacttta ttggcggtgc cgctggttcg ctaatttcag
     4501 cctcagcctg gcaacatggc ggttgggctg gcgtgtgtct ggctggcgcg acgattgccc
     4561 tggttaactt actggtctgg tggcgaggtt ttcatcgtca ggaagccgca aattaagcaa
     4621 tcagcaaccg ttttcgtggc tttacactta taagggtgtt aagaagccca tcagtctgat
     4681 aaggttaaga tattcattca gtctatttat aatattaaca atcgttaagc gtacactcta
     4741 tggaaagccc tactccacag cctgctcctg gttcggcgac cttcatggaa ggatgcaaag
     4801 acagtttacc gattgttatt agttatattc cggtggcctt tgcgttcggt ctgaatgcga
     4861 cccgtctggg attctctcct ctcgaaagcg tttttttctc ctgcatcatt tatgcaggcg
     4921 cgagccagtt cgtcattacc gcgatgctgg cagccgggag tagtttgtgg gttgctgcac
     4981 tgaccgtcat ggcaatggat gttcgccatg ttttgtatgg cccgtcactg cgtagccgta
     5041 ttattcagcg tctgcaaaaa tcgaaaaccg ccctgtgggc gtttggcctg acggatgagg
     5101 tttttgccgc cgcaaccgca aaactggtac gcaataatcg ccgctggagc gaaaactgga
     5161 tgatcggcat tgccttcagt tcatggtcat cgtgggtatt tggtacggta ataggggcat
     5221 tctccggcag cggcttgctg caaggttatc ccgccgttga agcagcatta ggttttatgc
     5281 ttccggcact ctttatgagt ttcctgctcg cctctttcca gcgcaaacaa tctctttgcg
     5341 ttaccgcagc gttagttggt gcccttgcag gcgtaacgct attttctatt cccgtcgcca
     5401 ttctggcagg cattgtctgt ggctgcctca ctgcgttaat ccaggcattc tggcaaggag
     5461 cgcccgatga gctatgaggt tctgctgctt gggttactgg ttggcgcggc gaattattgc
     5521 ttccgctatc tgccgctgcg cctgcgtgtg ggtaatgccc gcccaaccaa acgtggcgcg
     5581 gtaggtattt tgctcgacac cattggcatc gcctcgatat gcgctctgct ggttgtctct
     5641 accgcaccag aagtgatgca cgatacacgc cgtttcgtgc ccacactggt cggcttcgcg
     5701 gtactgggtg ccagtttcta taaaacacgc agcattatca tcccaacact gcttagtgcg
     5761 ctggcctatg ggctagcctg gaaagtgatg gcgattatat aacccacaag aatcattttt
     5821 ctaaaacaat acatttactt tatttgtcac tgtcgttact atatcggctg aaattaatga
     5881 ggtcataccc aaatggatag ttcgtttacg cccattgaac aaatgctaaa atttcgcgcc
     5941 agccgccacg aagattttcc ttatcaggag atccttctga cccgtctttg catgcatatg
     6001 caaagcaagc tgctggaaaa ccgcaataaa atgctgaagg ctcaggggat taacgagacg
     6061 ttgtttatgg cgttgattac gctggagtct caggaaaacc acagtattca gccttctgaa
     6121 ttgagttgtg ctcttggatc atcccgtacc aacgcgacgc gtattgccga tgaactggaa
     6181 aaacgcggtt ggatcgaacg tcgtgaaagc gataacgatc gccgctgcct gcatctgcaa
     6241 ttaacggaaa aaggtcacga gtttttgcgc gaggttctac cgccgcagca taactgcctg
     6301 catcaactct ggtccgcgct cagcacaaca gaaaaagacc agctcgagca aatcacccgc
     6361 aaattgctct cccgtctcga ccagatggaa caggacggtg tggttctcga agcgatgagc
     6421 taacgcgtca tctcgctcaa aaatccagat ttataaaaga aaaaataacc ggccagcatc
     6481 gtaacatgct ggcctttttg gcaagcaggt cggctcagcc gatgagttaa gaagatcgtg
     6541 gagaacaata tgagcgcaaa tgcggagact caaaccccgc agcaaccggt aaagaagagc
     6601 ggcaaacgta agcgtctgct cctccttctc accttgctct ttataattat tgccgtagcg
     6661 atagggattt attggttttt ggtactgcgt catttcgaag aaaccgatga cgcatacgtg
     6721 gcagggaatc agattcaaat tatgtctcag gtgtctggca gcgtgacgaa agtctgggcc
     6781 gataacaccg attttgtaaa agaaggcgac gtgctggtca ctctcgaccc gacagatgct
     6841 cgccaggcat ttgaaaaagc caaaactgca ctggcttcca gcgttcgcca aacccaccag
     6901 ctgatgatta acagcaaaca gttgcaggcg aatattgagg tgcaaaaaat cgccctcgcg
     6961 aaagcacaaa gcgactacaa ccgccgtgtg ccgctgggca atgccaacct gattggtcgc
     7021 gaagagctgc aacacgcccg cgacgccgtc accagtgccc aggcgcaact ggacgtcgct
     7081 attcaacaat acaatgccaa tcaggcgatg attctgggga cgaaactgga agatcagcct
     7141 gccgtacaac aggctgccac cgaagtacgt aacgcctggc tggcgctgga gcgtacccgt
     7201 attgtcagtc cgatgaccgg ttatgtctcc cgccgcgcgg tacagcctgg ggcgcaaatt
     7261 agcccaacga cgccgctgat ggcggtcgtt ccagccacca atatgtgggt ggatgccaac
     7321 tttaaagaga cgcagattgc caatatgcgt atcggtcagc cggtcaccat caccaccgat
     7381 atttacggcg atgatgtgaa atacaccggt aaagtggttg gtctggatat gggcacaggt
     7441 agcgcgttct cactgcttcc ggcgcaaaac gccaccggta actggatcaa agtcgttcag
     7501 cgtctgcctg tgcgtattga actggaccag aaacagctgg agcaataccc gctgcgtatc
     7561 ggtttatcca cgctggtgag tgtcaatacc actaaccgtg acggtcaggt actggcaaat
     7621 aaagtacgtt ccacaccggt cgcggtaagc accgcgcgtg aaatcagcct ggcacctgtc
     7681 aataaactga tcgacgatat cgtaaaagct aacgctggct aatccagagg tgcgtgtgat
     7741 gcaacagcaa aaaccgctgg aaggcgcaca actggtcatt atgacgattg cgctgtcact
     7801 ggcgacattc atgcaggtgc tggactccac cattgctaac gtggcgatcc ccactatcgc
     7861 cgggaatctg ggctcatcac tcagtcaggg aacgtgggtg atcacttctt tcggggtggc
     7921 gaatgccatc tcgatcccgc ttaccggctg gctggcaaag cgcgtcgggg aagtgaaatt
     7981 gttcctctgg tcaaccatcg cctttgctat tgcgtcgtgg gcgtgtggtg tctccagcag
     8041 cctgaatatg ctgatcttct tccgcgtgat tcaggggatt gtcgccgggc cgctgatccc
     8101 gctttcacaa agtctattgc tgaataacta cccgccagcc aaacgctcga tcgcgctggc
     8161 gttgtggtcg atgacggtga ttgttgcgcc aatttgcggc ccgatcctcg gcggttatat
     8221 cagtgataat taccactggg gctggatatt cttcatcaac gtgccgattg gcgtggcggt
     8281 ggtgttgatg acactgcaaa ctctgcgcgg acgtgaaacc cgcaccgaac ggcggcggat
     8341 tgatgccgtg gggctggcac tgctggttat tggtatcggc agcctgcaga ttatgctcga
     8401 ccgcggtaaa gagctggact ggttctcatc acaggaaatt atcatcctca ccgtggtggc
     8461 ggtggtggct atctgcttcc tgattgtctg ggagctgacc gacgataacc cgatagtcga
     8521 tctgtcgttg tttaagtcgc gcaacttcac catcggctgc ttgtgtatca gcctcgcgta
     8581 tatgctctac ttcggcgcga ttgttctgct gccgcagttg ttgcaggagg tctacggtta
     8641 cacggcgacg tgggcagggc tggcctctgc gccggtaggg attattccgg tgatcctgtc
     8701 gccgattatc ggccgctttg cgcataaact ggatatgcgc cgactggtaa ccttcagctt
     8761 tattatgtat gccgtctgct tctactggcg tgcctatacc tttgaaccgg gtatggattt
     8821 tggcgcgtcg gcctggccac agtttatcca gggttttgcg gtggcctgct tctttatgcc
     8881 gctgaccacc attacgctgt ccggtttgcc accggaacga ctggcggcgg catcgagcct
     8941 ctccaacttt acgcgaacgc tggcggggtc tatcggcacg tcgataacca cgaccatgtg
     9001 gaccaaccgc gagtcactgc accatgcaca gttgactgag tcggtaaacc cgttcaaccc
     9061 gaatgctcag gcgatgtaca gtcaactgga agggcttggt atgacgcaac agcaggcatc
     9121 aggctggatt gcccagcaga tcaccaatca ggggctgatt atttccgcca atgagatctt
     9181 ctggatgtca gccgggatat tcctcgtcct gctggggctg gtgtggtttg ctaaaccgcc
     9241 atttggcgca ggtggcggcg gaggtggtgc gcactaagta caactaagcc agttcatttg
     9301 aactggcttt tttcaattaa ttgtgaagat agtttactga ctaaatgtgc agttcctgca
     9361 acttctcttt cggcagcgcc agttcttcgt tgctgttgat gcgcacgtca cgttccagaa
     9421 tgttacgcgc aatatcctgc gcttcctgca atgagtgcat ctggtaagtg ccacactggt
     9481 agacgttcag ttccgggatc tgattctgat cctgcacttt cagcacgtct tccattgccg
     9541 ctttccaggc atcagcgaca cgctgctcat ctggcgtacc aatcagactc atataaaaac
     9601 cggtgcggca gcccattggc gagatatcga taatctctac gccattaccg ttaagatggt
     9661 tacgcataaa accagcaaac aggtgctcca gggtatggat ccctctttct ggcatcactt
     9721 ctttgttcgg cacgcagaag cgcagatcga acacggtgat tgcgtcgcca tgcggggtgt
     9781 tcattgtttt tgccacccga actgcaggcg cttccatccg ggtatgatcg actgtgaagc
     9841 tatctaacaa cggcatttag ccacctccgg taattttttt aaaaattttc tgaactcttt
     9901 gttcccaggc gagtctgagt atatgaaaga cgcgcatttg ttatcatcat ccctgaattc
     9961 agagatgaaa ttttggccac tcacgagtgg cctttttctt ttctgtcagg cgtgcttttc
    10021 cagccacacc gcaaacggtt cggtatcagc ggcttccatt tcctgctgac ggcgttcaga
    10081 cgcctcgcgc tcggcgacaa aatcctcttc gcgcagaatt tccagcggct cttcacgcag
    10141 cagattacgg tacgcttcag caaacgcttt gcctgttccg ccaataccag tatcaatcat
    10201 agaccttaag atacgggcag agaaagtcag atcaggatta tcgaagcagg cgaccagttc
    10261 atcacacact ttctgatacg cttcgccgcc gttaatgcta tccagcgttt gcgcgacgcg
    10321 tttcagatcg cggaacagat ctttacccac ctgcggtaac gggaactgcg cagtttcgca
    10381 gccgataccc agcgtcaggc ccggtttgcg accttcgagg atcacccggt tccagttaac
    10441 gcgtgtacag gcaagttcgc tactgctcat ttccggtgca tcagccagcg cacaccagac
    10501 cataaacagg tcgaggaatc gcacctgttg ttcatctaca ccaatcggcg agaacgggtt
    10561 gatgtccaac gagcggactt caatatattc aatgccgcca cgtaacagcg catcagaagg
    10621 cgactcgccg ctgcgggtaa cgcgttttgg acgaatcggc gcgtacagtt cgttttcaat
    10681 ctgcagcacg ttgctgttga tttgcagccg cttaccgtct ttctcaatac caatcttcgc
    10741 gtactcttcc gatggcgttt tgattgcctg tttaaggccc gccacgtact cgtagagatc
    10801 attgaaggtg ataccaagat tgctttgcga tttattggta tagccgagat cgctcaaacg
    10861 aagagaggtc gcatacggca ggtaatacat accgcactcg gttttctcaa acggcagcga
    10921 cgttggtttt ccttgcaaga aagaagaaca aatcgctgga gacgcaccaa acagataagg
    10981 aatgacccaa ccgaagcgat agtagttacg gataacacgg aaataacccg cagatatttt
    11041 ctctttggca tcagcgcccg agatatcacc gcacttcgct tgccagaatg ccattggcaa
    11101 agagaaatta tagtgcacgc cggaaatggt ttgcatcagc gcgccgtagc gatttttcag
    11161 cccttcacgg tacagcgttt taaagcgacc ggtgttagaa gtgccgtact gtgccagttc
    11221 gatgtcctga ccttctgcga tatagcatgg catacttaac ggccacatcc gctcatcgcc
    11281 catattgcgc gccgtatagc gatgcagatc gcgcataaag gtcagcatat gttcaatatc
    11341 accatccact ggtgtaataa attccagcaa tgcttccgca aaatcggtag taatccattt
    11401 atgggtcagt gcagaaccta atgcttcagg atgacctgtt gttgccagtg tgccatcagc
    11461 attaacacgc aaagtttcgc gctccagccc acgctgtatc ccctttaacg cctgaggatg
    11521 tttttccagc caggccagcg cctgtgatac gtccgggatc aaattgacct cccgcctgtc
    11581 aaaatcgttt taattagcat aactgtaatg gtgaccatat gtgcaggcct acaattagtg
    11641 ccaccacatc atgccctgaa cggtagctgc tgcaactgca acatagcgta acgctttacc
    11701 aaggcacaaa aaaaagatta ccggtcccca cgagatgcgc atccatcccg ctaacagaca
    11761 cagtaaatcg ccaaccacgg gcatccagct taataatagc gtgactgcac cattgcgttt
    11821 cagccagccg gtagctttct cttgccagcg cgatgtttta cgcaatggaa agaaacgccc
    11881 aaggataacg ttagttaacc ctccaaggct attacccatt gttgctgtta aaactaaaac
    11941 ccagggatga ctcactccgg aaagcaacat tgccaccagc acgacttcgg agttgccggg
    12001 taatagtgta gcgctgagaa aactactggc aaacaacgag aaaagcgata acgcttcact
    12061 cacagcaagc gaacatccac ggcgtccatg cctgctgcac gggccgcctg aataccgaaa
    12121 tcggcatctt caaagaccac acactgcgtc ggttgcacgc ccatacgctg cgcgcacaac
    12181 aaaaatgtgt ctggcgcggg tttatggtgt ttgacgtgat cggcagcgac gacggcgtca
    12241 aaataacggc gtaatcccag gtgtgccagc aatgcctcag cgatggcgct ttcactcccc
    12301 gttcctacag ccattgggcg acgaccatgc cagcttttca ccacttcaac aagaggaagc
    12361 ggctcgacac tatccagcag catacttctt actgcttctg ttttttcacg cgctaacgca
    12421 tgcgggtcga gatcggcctg attcagctca ataatcgcct gagcaatacg ccaggtgggc
    12481 gagccattaa gcgcaatcat cgcctgaaca tcgtactgaa gaccgtagtg ccctaatact
    12541 tcgcgccacg ctttacggtg cgtaggctcg gtatccagga tggtgccatc catatcaaaa
    12601 attaaacctg cataacgctc gtacatggtc ttctcgcaag tagaaaaata tgacgctact
    12661 ttagcgtaat tgcttgattt tgtcgctgat ggagaaggag aagaaaattc aggaaatgga
    12721 taaagtagtt atttagaaat gagatatttt tgaaggaaat ttttttgaga agatggtgca
    12781 tccgggagga tgactcactg gcgttcgccc ttcgggccgc tgctggcgca acgttatcct
    12841 ccctggtgcc tgcggttaat accgctacct cttcgatacc ttcattgctg aaggttacgg
    12901 agaa
//
LOCUS       DADSZD010000126        12877 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.127, whole genome shotgun sequence.
ACCESSION   DADSZD010000126 DADSZD010000000
VERSION     DADSZD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.127"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(124..2391)
                     /gene="adiA"
                     /locus_tag="J1Z28_003283"
     CDS             complement(124..2391)
                     /gene="adiA"
                     /locus_tag="J1Z28_003283"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBA7718093.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESERYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKVGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYSPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2590..3498)
                     /gene="melR"
                     /locus_tag="J1Z28_003284"
     CDS             complement(2590..3498)
                     /gene="melR"
                     /locus_tag="J1Z28_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBA7718094.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3781..5136
                     /gene="melA"
                     /locus_tag="J1Z28_003285"
     CDS             3781..5136
                     /gene="melA"
                     /locus_tag="J1Z28_003285"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBA7718095.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELVRDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            complement(5133..5783)
                     /locus_tag="J1Z28_003286"
     CDS             complement(5133..5783)
                     /locus_tag="J1Z28_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBA7718096.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRRRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(5906..7552)
                     /locus_tag="J1Z28_003287"
     CDS             complement(5906..7552)
                     /locus_tag="J1Z28_003287"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBA7718097.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(7630..8970)
                     /locus_tag="J1Z28_003288"
     CDS             complement(7630..8970)
                     /locus_tag="J1Z28_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBA7718098.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(9541..10260)
                     /gene="dcuR"
                     /locus_tag="J1Z28_003289"
     CDS             complement(9541..10260)
                     /gene="dcuR"
                     /locus_tag="J1Z28_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBA7718099.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(10257..11888)
                     /gene="dcuS"
                     /locus_tag="J1Z28_003290"
     CDS             complement(10257..11888)
                     /gene="dcuS"
                     /locus_tag="J1Z28_003290"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBA7718100.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            12069..12299
                     /gene="yjdI"
                     /locus_tag="J1Z28_003291"
     CDS             12069..12299
                     /gene="yjdI"
                     /locus_tag="J1Z28_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBA7718101.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            12311..12583
                     /locus_tag="J1Z28_003292"
     CDS             12311..12583
                     /locus_tag="J1Z28_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7718102.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
BASE COUNT         3131 a         3116 c         3372 g         3258 t
ORIGIN      
        1 ccgcgcaaaa agcatgacat tttttgtttg cagcaatttt tatgaaaacg ttgccatact
       61 taaaaggcct caagccaggc ggcgtaatgt tatttaacaa ttacgccttc agcggaatag
      121 tggttacgct ttcacacaca taacgtggta aataccgtca ataatttctg tcccttctgt
      181 ttcgtgttca aatccaggga aatgatggtc ccaggattgc agcgagcgta aataacttac
      241 ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcga
      301 atacgggata actgagtttg ccgcgatacg tcctggcaga ttttcaatgg ataccagttc
      361 gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccaccggtag
      421 gccggaatag gcttcattca accgtgcgcc agggttgttt tctttcagcc aggcaaacat
      481 ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag
      541 ttcaggcatc acctgcgcca gcggtgtgtt ggcatcatag tggcgtttga aggagcaaag
      601 ggtgttaacc agagtccccc atttcccacg ggtcacgccc atagagaaca ggaacataat
      661 ctggaagtca gtggtacggg taggcacaat gccgtggcga ccaagccagg ctgtgaccag
      721 cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat
      781 gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga agccgtgcca
      841 gctttcgccc ggatgcatga cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc
      901 gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccatgg
      961 tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca tcgcctgacg
     1021 gaaatcaacc gcttcgtcga tcacttcctg ggtcagtgac aggccgctgt tgccgtccat
     1081 catcgacact gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg
     1141 catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg
     1201 aatataagag gcctgggaca gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac
     1261 ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg
     1321 gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc
     1381 cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtac agttggtcac
     1441 cacgcagtaa gacggttttt gcccgacttt gtctttggtc agcgggcttt cactgatttt
     1501 cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg
     1561 gttgcggctt ggcaccatgt agaccggttt cgcgcctgtc agcatcaagc cttgttcgat
     1621 ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat
     1681 gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa
     1741 tacgcgtgcg gcatatcttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc
     1801 gagggaagtg cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat agtagtcatg
     1861 gtagaaacgt ccggcgggtg ttttagtaaa accaacgccg ccctggtggc ctggcgctgc
     1921 ccaggaatat tcatggatgt cgctatattt catcagcgcg ctgaacagtg gcggcaatag
     1981 ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt cggcagtatc
     2041 ttccagaatc caggcgaatt catcgacaag ctccagcagg tcgcgatcca ttgcggcgag
     2101 ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt
     2161 accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat agctgaacat
     2221 caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga
     2281 tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc
     2341 gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta
     2401 cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa
     2461 gcgcgtgaaa aacaaacatt tttgaaatat ttatttttat aaacaacacg ttatgtgatt
     2521 attttaaatt attttcactg cttattatcg acggctaaac tatttttttg gctgatactg
     2581 atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg
     2641 cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cggctactcg aacgaaagcc
     2701 tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg
     2761 gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat
     2821 ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc
     2881 ctgatcatag ttttcggcaa taaagcccag catctggctg acataaaatt gcgcatggcg
     2941 cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga
     3001 gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc
     3061 gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt
     3121 cgccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg
     3181 ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt
     3241 atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct gattgatatt
     3301 cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac
     3361 ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tttccatccg
     3421 ctggtattct gaatacagcg acagtgggct acgggtctgc ttttcgtcgc tgctgcacat
     3481 aaacgtatct gtattcatgg atgactctct ttcctggaat atcagaatta tggcaggagt
     3541 gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccacag cggcgagggg
     3601 agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt cccataaact
     3661 cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagacg
     3721 gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc
     3781 atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat
     3841 attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac
     3901 attgatccca cccgcctgga agagtcgcac attgtggtgc gtaagctgat ggattcagca
     3961 ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgctgat
     4021 tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag
     4081 gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgctggggcc gggcggcatt
     4141 atgcgtgcgc tacgtaccat tccgcacctg tggcagattt gcgaggacat gacggaagtc
     4201 tgccccgatg ccaccatgct caactacgtt aacccaatgg cgatgaatac ctgggcgatg
     4261 tatgcccgct atccgcatat caagcaggta gggctgtgcc attcggtgca gggaacggcg
     4321 gaagagctgg tgcgtgacct caatatcgat ccggctacgc tgcgttaccg ttgcgcaggt
     4381 atcaaccata tggcgtttta cctggaactg gagcgtaaaa ccgcagacgg tagctacgtg
     4441 aatctctatc ctgaattgct ggcggcttat gaagcagggc aggcaccgaa gccaaatatt
     4501 catggtaata ctcgctgcca gaatattgtg cgttacgaaa tgttcaaaaa gctgggctac
     4561 ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat taagccaggt
     4621 cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc
     4681 gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat
     4741 attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac tggcgagccg
     4801 agtgtgattt atggcaatgt ccgtaacgat ggcttgattg ataacctgcc acaaggatgt
     4861 tgcgtggaag tggcctgtct ggttgatgct aatggcattc agccgaccaa agccggtacg
     4921 ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac gctgctgaca
     4981 gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat
     5041 actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct gattgccgcc
     5101 cacggcgact ggctgccagg ctggttgcac cgttaagggg taatcgcttc cggcgctctg
     5161 gtgattaagc caaagcgccg tagctggcgg caatgaaagc gggcgaggaa tatcaccgtc
     5221 gttaatgcgc ccagtagtgc gcagaacata tcggattgcg tatcccacgg gtcgccttga
     5281 gtaccgagaa aatcatctgc gccctggccc attgccagcg ctgcccacca ctcgattaac
     5341 tcatacatcg cgcttatcgc cagcgcgaca caacagacca gaaacgccac cattttacgc
     5401 ctgcgaacgt acatcccgcg cacgaggatc tctcgtgcca ccagtgcagg caccagcccc
     5461 tggaaaaaat gccccagttt gtcatacgga ttacggctca accccagcca ttcctgcacc
     5521 tcaaaaccaa cggggacttt cgcgtaggtg tattgcccgc cgaccatcag gatgatggcg
     5581 tgaaagaaaa tgagcgtata gagcagcggc gttaacgggt aacgtttagc ggtggcaagc
     5641 agtagcggca cgacaataat caccggtgtc acttccatca gccaggtgag tttgtcatgg
     5701 gccgaaatgc cggtataaat caggattaac gttagcgcca gtgcgccggt gttaagaatt
     5761 aacggcttga gtgtgcgggt catggtgagt tcgcaagtca gggaaaatac tgactattga
     5821 ccagtaagcg tgaaattaca atcgcaaaag aggtggccca gggggatcac ctggcagcat
     5881 gctgccaggc gctgggccga agaggttact tagtgcagtt cgcgcactgt ttgttgacga
     5941 tctgctggaa gaagtcgtta cctttgtcat cgaccaggat aaacgccggg aaatcttcta
     6001 cttcgatttt ccagatagct tccataccca gctccggata agcgacgcat tccagatgct
     6061 tgatgctctg ctgcgccagt accgccgccg gaccgccgat gctaccgagg tagaagccgc
     6121 cgtgtttatg gcacgcgtcg gtaacctgct ggctgcggtt acctttcgcc agcatgatca
     6181 tgctgccgcc gtgggattgc agcagatcca cgtaggagtc catacgacct gcggtggttg
     6241 ggccaagtga acctgatgga taaccggcag gcgttttcgc cggacccgcg tagtagatcg
     6301 ggtgatcttt gatgtactgc ggcagttctt taccggcgtc aatcagctct ttcagcttgg
     6361 cgtgtgcaat atcgcgaccc acgataatgg tgccggtgag cgacaagcgg gtggataccg
     6421 ggtattgcga aagctgggca aggatctctt tcatcgggcg gttaaggtcg actttcactg
     6481 cttcgccttc acctgcctga cgcagttctt ctggaatgta ctggcctgga ttgtgttcca
     6541 gtttttcgat ccagatacct tcgcggttga ttttcgcttt aatgttacgg tcagcggagc
     6601 aggagactcc catgcctacc gggcaggatg cgccgtgacg tggcagacgg ataacgcgga
     6661 tgtcgtgcgc gaagtattta ccgccaaact gcgcgccaag accgagtttc tgggcctctt
     6721 ccagcagttc ctgttccagc tggatatcgc ggaacgcctg accatgttcg ttcccttccg
     6781 tcggcagttc atcgtaatag tgagcgcttg ctaacttgac ggttttcagg ttggtttccg
     6841 cagacgtacc gccaatcaca aacgcgatat ggtacggcgg gcaggctgca gtaccgaggg
     6901 tacgcatttt ctcgacgagg aagtttttca gtttgccggg cgtcagcagg gctttggttt
     6961 cctggtagag atacgttttg ttggcagagc cgccgccttt agcgacacag aggaatttgt
     7021 actcatcgcc atctaccgcg tacaggtcga tttgcgcagg caggttcgtg ccggtgttga
     7081 cctctttata catgtccagc ggcgcgttct gtgaatagcg caggttatct tcgatatagg
     7141 tgttatagac gcctttcgac agcgcttctt catcaccgcc accggtccac acgcgctgac
     7201 cttttttacc gacgatgatc gcggtgccgg tatcctggca ggtcggcagc acgcctttgg
     7261 cggcgatttc ggagtttctt aagaattgca gcgccacgta cttgtcgttt tcgctggctt
     7321 ctggatcgtg aagaatagcc gcaacctgtt tctggtgtgc cgggcggagc atgaaagaag
     7381 catcgtgaaa ggcttgctgc gccagcaggg tcagggcttc tggttccact ttcaggatgg
     7441 tttcgccgtc gaagtcggca acgctaacgt aatcggaagt gagtagatag tattcggtat
     7501 tgtctttccc catcgggaaa ggtgcctggt agataaaggg tttgtttgac atagcttcca
     7561 gcctgtaact ctgtatttgt tattcgaaaa tggcgtgccg cgtagtcacg ggcggcacgc
     7621 aaagtgcatt tataagaacc cgtacatcgc ggcgaagatc cagccgaaga cgcacgatac
     7681 gctcacacca atcaaccccg gcagaataaa gctgtggttg atgacgaagc gaccgatgtg
     7741 ggtggtgccg gaacggtcaa actgaatcgc tgccagatcg ctcggataag tcggcaggat
     7801 gtaataaccg tagcaagccg gtgctgaagc cacgatgtaa gcggggtcaa cgccgatcgc
     7861 cagcgcgacc ggaacaatcg ccgccagcgc tgcagcctga gagtttacaa acttggaaac
     7921 cagcagcaga acaatggcat aggcccacgg atactctttc accatttcac ccagtacgcc
     7981 ctgaatttca gacatatgcg caccgaacat ggtttcagcc atccatgcga taccgtacac
     8041 cgccacgatg gcgatcatac cggaacggaa aacttcgttt tttgagatag acgcgggatt
     8101 ggttttggtc aggataataa tcagcgcccc ggtcagcagc ataaacatct gaataaccag
     8161 taccatcgac agcggtttgc cgccgaagga tggacgcagg tccgaatcag caccaagaag
     8221 ggcgactaca gcgattgccc cgaggaagat ccacattgcc agccagttgc ttttcggcag
     8281 ttttttatcc agcagcgtcg cggtatcacc gtaaacatac tcacggtttt ccggtacgga
     8341 gatgaatttt tggaactctt cgtctttatc cagatcttta ccgcggaacc agctgaagat
     8401 accgatcgcc aggataccga ttaacgtcga tggaatggtg attgccagca ggtcgaggaa
     8461 ctcaagatgg cgaccatcaa aggtgacatt acccagcatc gcaaccagag atacgaccgc
     8521 aaccgacacc ggactggcga taatccccat ctgtgcaccg atagaacttg ccgccatcgg
     8581 acgttccgga cggatgttgt tcttaatagc aacgtcgtag atgatcggca gaatggtgta
     8641 aaccacatga cccgtaccgc aaagaatggt cagcgtacag gtcacaaacg gcgcgacaat
     8701 tgagacatat ttcgggttgc gacgcagcag cttctcggca atttgcagca tgacatcaag
     8761 accgcccgaa gcttgcaagg tcgccgatgc cgccaccacc gcaatgataa ccagcatgac
     8821 atcaaccggt ggtttacctg gctgaaggtg gaagacgaag accagaatga ccagaccgat
     8881 accgcctaat aaacccagcg cgataccccc ctttctggca ccataaaaca gacatatcag
     8941 tattatgata agttggatag taaataacat gtgtgaaccc tcgcgataat cctatttaaa
     9001 tttttgctga atagatcaca gtcacgttct gttttgtatg aactgttttc agagcctgac
     9061 taaatatccg tttggtcgtt accggcttga gcaaatacct cacagtgaat attggctgat
     9121 taaagcagcg gcaaatttat gcactgtgat aagcggcttt ttcagagaga aagactctct
     9181 ggcgatgggt ttatagtttg tacagcgcag atgttaatgt tttgtttgct gttatcttat
     9241 tgatatttat cggtctgttt tttgttctca gggcaggtat cattaggttt atagattaat
     9301 ctgatctacc catttgtggg taaaaataca cataatgtgg gtgacataat agttaattaa
     9361 cttttgttag cgttttgaaa ttaaaaacac cgttcatctg aagagatatt aatttttagc
     9421 gatgatggag ggataattat atttgatctg gcacaagttt tactgatgaa gaatgtaact
     9481 tgtgccagga atattttgca ttacggtaat aattattact tacagataac cagcgatccg
     9541 ttattggcaa tattgtttca gcagtgagta gtgttcagcc tgaatacggt aacggtaaac
     9601 cggacgcccc gtaacgccat aatggatact ggtgaacaag atgtggcagt tgaccagcca
     9661 gatgaggtat ttacggcagg aaacacgcga gatgttaacc tcgttggcca gctcgtcggt
     9721 tgaaaactca tagtcctgat gcgcgtcaat ccactggcac agcgtgcgta acgtctgcgg
     9781 cgttaagcct tttggcaagc gacgaggatc ctgttcgttg gagctgctgc cgtggattag
     9841 ctgatcaagc tcggcctggt cataatactg atgtttttcc agcgccattt tcttttgccg
     9901 ccagccggtg agcgcctctt caaagcggga agcctggaag ggtttgatca ggtaatccac
     9961 aacaccgtaa tgcagcgaat ctttaatggt tactgcatcg gcagcggagg agataacaat
    10021 cacatcactt ttgcaacgcg cgttatgcag gacaggcagt aaatcgagcc cgttctcttt
    10081 ttgcatatag atatctagca atatcaggtc gataggcgta tcgctattga agataatctc
    10141 tttggctttc tccagcgtcg aagccgttcc acagcattga aagcccggga tttgtgccac
    10201 gtatcggcga ttcagctccg cgaccattgc gtcatcatcg ataattaata cattgatcat
    10261 ctgttcgacc tctccccgtc ccagggtatc tggacaaaaa attgtgtgaa aatcccgggt
    10321 tccgattcca cggcgatgct gccgccgaga ttttctacct gttgtttgac aagtgctaaa
    10381 ccgacgcctc gctcgcttcc ttttgtcgag acacctttgt caaaaatgtg atcgattttg
    10441 tcgggtgcga tccccggtcc atcatcgtta acttcgcagt gcagccagcc gtgacggtag
    10501 tgcaatgtta cgctaatttc gccaccgggt tccggcccta atgcctccag cgcgttttct
    10561 atcagatttc ccaacgtggt aatcagcgtc gcgacctgat cctcactacc gctgtctggc
    10621 agctggcttt cactgtttaa aatcagcgta tggcctaaat cggtcgcgcg gttaatcttg
    10681 ctgattaaaa aaccagcgat aaccggagat ttgatcttac ccagcagaga gccaatctct
    10741 tcctgatagt tattggctgt tttgagaatg taatcttcca actgcttata actcttcaga
    10801 tgcaataatc cgagaatcac atgcaattta ttcataaatt cgtgggatcg ttcacgaagt
    10861 gcgtcagcat agttgaccag accgtcgagt cgctgcatca gtttacgtac ttcagttttg
    10921 tccctgaagg ttgaaatggc accgatgata acgccattac tgcgcaccgg aacggtgttg
    10981 atcagtaata gtcggtcctt aatcgtaatc tcttcgtcgc ggcgtggggt tccgtcgcgt
    11041 aacacttccg agacatctac cacctgtgac cacgagtggc ttagcgtcga cagtttctca
    11101 tcgtcctgcg acttacggta attcagcaat tcttgtgcgg catcgttgat cagcgtgacc
    11161 tcgccgcgat cgtccacggc aacgacgcct tctttgatgg actgcaacat ggcctggcgt
    11221 tgctcaaaca gtgtggagat ttcgtagggt tccaggccga aaaggatttt tttcagtacg
    11281 ttaaccagaa tgcatgtgcc gatcagtccg accagcatgc caaatagtac cgaccagata
    11341 atgctccagc gactgtcatt gatctgttgg gtcacgcggc ttaactcaag gccgatcgcc
    11401 accacgccaa tttgtttatg attttcatcg tagatggggg taaatacgcg taaagcctgc
    11461 gccagaaaac cgcgattgat agcgacattt tcttcgccat tcagcgcttt aaggatgtca
    11521 tcacctttaa atggctgacc aatacgctgg gcttcaggat gcgagtagcg aagactgtgc
    11581 atatcggtaa cgacaataaa cagcagatcg ttgcgtttgc gcacggcttc cgcgatggcc
    11641 tggattccac tctcctgcgg ttttttctgc aagccctgac ggatttccgg cgagtcggcg
    11701 agggtacgcg ctactgccag agccttgtta gctagcccgt ctcgcgtcat atcactgatt
    11761 tgcgagaagt aaatcagatg caccaccaat agcaccgaga acagtaccgc gctgaccatt
    11821 aagatcacag tggtactcaa tttcatcgga cgtttgcgta acatacggta gggcaatgaa
    11881 tgtctcatca gcttccttgt gtgacaaatt tcttaagcat tatctctgat gaggcgggta
    11941 attcaaaggg agtaagaatg attggctata taggggaaga gattctggca acggaaactg
    12001 ccagtgctgt atgaagattc cggggctatg cttatagcga taatcatact gatgagagag
    12061 ggaaggtcat ggatcgggcg ctactggacg ggggttatcg ctgttatacc ggcgaaaaga
    12121 tcgatgtcta tttcaatact gcgatatgtc agcattctgg caattgcgta cgtggcaacg
    12181 gcaagttatt taatctcaaa cgaaagccgt ggatcatgcc ggatgaagtc gacgttgcca
    12241 ctgtggttaa agtgattgat acgtgcccga gcggcgcgct gaaataccgt cataaataag
    12301 cgagggtaaa atggaaatac gcgaaggcca caataaattt tacattaatg acgaacaagg
    12361 caagcaaatc gctgaaattg tctttgtgcc gaccggagag aatttagcga ttatcgaaca
    12421 taccgatgtc gatgaaagcc tgaaagggca agggattggt aaacagctgg ttgcgaaagt
    12481 cgtggaaaaa atgcgtcggg aaaaacgaaa aattatccca ctatgcccat ttgcgaaaca
    12541 cgaatttgat aaaacacggg agtatgatga tattcgcagt tgatgggaga gtacagagtc
    12601 acgatatttt tcatgctctc cgcggtgtga tgcaggagag catctgaagg gtagggggat
    12661 gcacaaagaa tggtcagaga gagcgttttt ttgtcccaag tcatcccctt tactgagcaa
    12721 aaaaaaaaga atatctccta tatgagaatc atcattcggg gttaataagt tttgcgtccc
    12781 cagagcgttc aatattgata ggagtcatat tatggaaggt aaaaacaagt tcaatactta
    12841 tgttgtttct tttgattatc catcatctta ttcctca
//
LOCUS       DADSZD010000127        12742 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.128, whole genome shotgun sequence.
ACCESSION   DADSZD010000127 DADSZD010000000
VERSION     DADSZD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.128"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            154..1212
                     /gene="citC"
                     /locus_tag="J1Z28_003293"
     CDS             154..1212
                     /gene="citC"
                     /locus_tag="J1Z28_003293"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBA7718103.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1227..1523
                     /gene="citD"
                     /locus_tag="J1Z28_003294"
     CDS             1227..1523
                     /gene="citD"
                     /locus_tag="J1Z28_003294"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBA7718104.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1520..2428
                     /gene="citE"
                     /locus_tag="J1Z28_003295"
     CDS             1520..2428
                     /gene="citE"
                     /locus_tag="J1Z28_003295"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBA7718105.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2439..3971
                     /gene="citF"
                     /locus_tag="J1Z28_003296"
     CDS             2439..3971
                     /gene="citF"
                     /locus_tag="J1Z28_003296"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBA7718106.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3975..4526
                     /gene="citX"
                     /locus_tag="J1Z28_003297"
     CDS             3975..4526
                     /gene="citX"
                     /locus_tag="J1Z28_003297"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBA7718107.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGDILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4501..5379
                     /gene="citG"
                     /locus_tag="J1Z28_003298"
     CDS             4501..5379
                     /gene="citG"
                     /locus_tag="J1Z28_003298"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBA7718108.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5430..6893
                     /gene="citT"
                     /locus_tag="J1Z28_003299"
     CDS             5430..6893
                     /gene="citT"
                     /locus_tag="J1Z28_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBA7718109.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7007..7813
                     /gene="rna"
                     /locus_tag="J1Z28_003300"
     CDS             7007..7813
                     /gene="rna"
                     /locus_tag="J1Z28_003300"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBA7718110.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGNTVSRRDFDAAFAKSWVKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8043..8453
                     /gene="rnk"
                     /locus_tag="J1Z28_003301"
     CDS             8043..8453
                     /gene="rnk"
                     /locus_tag="J1Z28_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBA7718111.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8809..9237
                     /gene="uspG"
                     /locus_tag="J1Z28_003302"
     CDS             8809..9237
                     /gene="uspG"
                     /locus_tag="J1Z28_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBA7718112.1"
                     /translation="MYKTIIMPVDVFEMELSDKAIRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAK
                     ELDADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9358..10923)
                     /gene="ahpF"
                     /locus_tag="J1Z28_003303"
     CDS             complement(9358..10923)
                     /gene="ahpF"
                     /locus_tag="J1Z28_003303"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBA7718113.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11094..11657)
                     /gene="ahpC"
                     /locus_tag="J1Z28_003304"
     CDS             complement(11094..11657)
                     /gene="ahpC"
                     /locus_tag="J1Z28_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBA7718114.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12029..>12742
                     /gene="dsbG"
                     /locus_tag="J1Z28_003305"
     CDS             12029..>12742
                     /gene="dsbG"
                     /locus_tag="J1Z28_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBA7718115.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQ"
BASE COUNT         2992 a         3362 c         3251 g         3137 t
ORIGIN      
        1 aatactcctt aggatgtata gcgaaaggag aaaaagatat acctcgatcg cccccctttc
       61 tcccaagtga aaataaaagg ttatcagttt gcaacatcga acaacatttg ttgcaaatcg
      121 ataacaacat gcaccttcag gatactattt attatgttcg gcaatgatat tttcacccgt
      181 gtaaaacgtt cagaaaataa aaaaatggcg gaaatcgccc aattcctgca tgaaaatgat
      241 ttgagcgttg acaccacagt cgaagtattt attaccgtaa cccgcgatga aaagcttatt
      301 gcgtgcggtg gaattgccgg aaatattatt aaatgcgttg ctatcagtga atccgttcgc
      361 ggtgaaggac tggcgctgac attagccacc gagctaatta acctcgccta tgagcggcac
      421 agcacgcatc tgtttattta taccaaaacc gaatacgagg cgctgttccg ccagtgcggt
      481 ttttccacgc tgaccagtgt acccggcgtg atggtgctga tggaaaacag cgccacgcga
      541 ctgaaacgct atgccgaatc gctgaaaaaa tttcgtcatc cagggaacaa gattggctgc
      601 attgtgatga acgccaatcc ctttacgaat ggtcaccgtt atctgattca acaggctgca
      661 gcacagtgcg actggttgca tctgttttta gttaaagaag attcttcacg ctttccctat
      721 gaagaccggc tggatctggt gttaaaaggc accgccgata ttccacgcct gactgtgcat
      781 cgcggctccg aatacatcat ctcccgcgct acgttccctt gctacttcat taaagaacag
      841 agcgtcatta accattgtta caccgaaatt gatctgaaga ttttccgtca gtacctcgct
      901 cccgcgctgg gtgtaactca ccgctttgtc ggtactgaac ccttttgtcg cgttaccgcc
      961 cagtacaacc aggatatgcg ctactggctg gaaacgccga ctatctccgc accgcccatc
     1021 gaactggttg aaattgagcg gctgcgttac caggagatgc cgatatccgc ttcccgggta
     1081 cgtcaactgc tggcgaaaaa cgatctcacg gctatcgcgc cgctggtccc tgcagtcacg
     1141 ctgcattatt tgcagaacct gcttgagcac tcccgccagg acgcggcagc tcgtcaaaag
     1201 acccccgcat gagaaacagg tgaaaaatga aaataaacca gcccgccgtt gcaggcaccc
     1261 ttgagtctgg ggatgtgatg atacgcatcg ccccactcga tacgcaggat atcgacctgc
     1321 aaatcaacag cagcgttgag aaacagtttg gcgatgcaat tcgcaccacc attctggacg
     1381 ttctcgcccg ctacaacgtg cgcggcgtac agctgaatgt cgatgacaaa ggcgcactgg
     1441 actgcatttt acgtgcacga ctggaagccc tgctggcacg cgccagcggt atcccggctc
     1501 tgccatggga ggattgccaa tgatttccgc ttcgctgcaa caacgtaaaa ctcgcacccg
     1561 ccgcagtatg ttgtttgtgc ctggtgccaa tgccgcgatg gtcagcaact ccttcatcta
     1621 cccggctgat gccctgatgt ttgacctcga agactccgta gcattgcgtg aaaaagacac
     1681 cgcccgccgc atggtttacc acgcgctgca acatccgctt tatcgcgata ttgaaaccat
     1741 tgtgcgtgta aacgcgctgg attccgaatg gggtgttaac gacctggaag ccgtcgttcg
     1801 cggtggtgcg gacgttgtgc gtctgccgaa aaccgatacc gctcaggatg ttctggatat
     1861 cgaaaaagag atcctgcgta tcgaaaaagc ctgtggtcgt gaacccggca gtaccggcct
     1921 gctggcggcg attgaatctc cgctgggcat tacccgcgcg gtagaaatcg ctcacgcttc
     1981 cgagcgtttg atcggtatcg ccctcggtgc agaagactat gtgcgcaacc tgcgtacaga
     2041 acgctccccg gaaggaactg aactgctgtt cgctcgctgc tccattttgc aggccgcgcg
     2101 ctctgcgggt attcaggcgt tcgataccgt ctattccgac gctaacaacg aagccggatt
     2161 cctgcaagaa gccgcccaca tcaaacagct gggctttgac ggcaaatcgc tgatcaaccc
     2221 gcgtcagatt gatctgctgc acaacctcta cgcaccgacc cagaaagaag tggatcacgc
     2281 ccgccgcgtc gtagaagccg ctgaagccgc cgctcgcgaa ggcctcggcg tggtttccct
     2341 gaacggcaag atggtggacg gtccggttat cgatcgcgcc cgtctggtgc tctcccgtgc
     2401 agaactttcc ggcatccgcg aagaataagg caatcaaaat gacgcagaaa attgaacaat
     2461 ctcaacgaca agaacgggta gcggcctgga atcgtcgcgc tgaatgcgat cttgccgctt
     2521 tccagaactc gccaaagcaa acctaccagg ctgaaaaagc gcgcgatcgc aaactgtgcg
     2581 ccaacctgga agaagcgatt cgtcgctctg gtttacagga cggcatgacg gtttccttcc
     2641 atcacgcttt ccgtggcggt gacctgaccg tcaatatggt gatggacgtc atcgcgaaga
     2701 tgggctttaa aaacctgact ctggcctcca gctccctgag tgattgccat gcgccgctgg
     2761 tagaacacat tcgccagggc gtggttaccc gcatttatac ctctggcctg cgcggtccac
     2821 tggcggaaga aatctcccgt ggtctgctgg cggaaccggt gcaaatccac tctcacggcg
     2881 gacgtgtgca tctggtacag agcggcgaac tgaatatcga tgtggctttc ctcggcgtcc
     2941 cgtcctgtga tgaattcggt aatgccaacg gctactccgg taaagcctgc tgcggctccc
     3001 tcggctatgc aatggttgat gccgacaacg caaaacaggt cgtgatgctt accgaagaac
     3061 tgctgcctta tccgcataat ccggcaagca ttgagcaaga tcaggttgat ctgatcgtca
     3121 aagttgatcg cgttggcgat gctgcaaaaa tcggcgctgg tgctacccgt atgaccacta
     3181 acccgcgcga actgcttatt gcccgtagcg ctgcggatgt gattgttaac tctggctact
     3241 tcaaagaagg tttctccatg cagaccggca ccggcggcgc ttcgctggcg gtaacccgtt
     3301 tcctggaaga caaaatgcgt agccgcgata ttcgcgccga cttcgccctc ggcggtatta
     3361 ccgcaacgat ggttgatctg cacgaaaaag gtttgatccg caaactgctg gatgtgcaga
     3421 gctttgacag ccatgctgcg caatcgctgg cccgtaaccc caatcacatc gaaatcagcg
     3481 ccaaccagta cgctaactgg gggtcgaaag gcgcagcggt tgatcgtctc gacgtggtgg
     3541 tactgagcgc gctggaaatt gacacccagt tcaacgttaa cgtgctgacc ggctctgacg
     3601 gcgtactgcg tggtgcttcc ggtggtcact gcgataccgc gattgcttct gcgctttcca
     3661 tcatcgtcgc gccgctggta cgcggtcgta ttccgacgct ggtggataac gtgctgacct
     3721 gcatcacccc gggctccagt gtcgatattc tggtcacaga ccacggcatc gctgttaacc
     3781 cggcacgtcc ggagctggca gaacgtctgc aggaagcggg gatcaaagtg gtttctattg
     3841 agtggctgcg tgaacgtgcg cgtctgctga ccggtgaacc acagccgatt gaattcaccg
     3901 accgcgtcgt tgccgttgtg cgttaccgcg atggctcggt gatcgatgtt gtgcatcagg
     3961 tgaaggaata agccatgcac ctgcttcctg aactcgccag ccaccatgcg gtgtcaattc
     4021 ccgagctgct cgtcagccgg gatgaaaggc aagcacggca acacgcctgg ctcaagcgcc
     4081 atcctgttcc actggtctcc tttaccgtgg ttgcgcctgg gccgattaaa gacagcgagg
     4141 tcacacgccg aatttttaat catggcgtga cagccttgcg tgccttagcc gcaaaacagg
     4201 gctggcaaat tcaggagcag gctgcactgg tttccgccag cgggccggag ggcatgttga
     4261 gcattgccgc cccggctcgc gacctcaagc tcgccaccat tgagcttgaa catagtcatc
     4321 ctctcgggcg gttatgggat atcgatgtcc tgacgcccga aggcgacatt ctctcccgcc
     4381 gcgattattc actgccgcct cgccgctgcc tgttgtgcga gcaaagcgca gctgtttgcg
     4441 cgcgtggaaa aacccatcaa ctgaccgatt tactcaaccg catggaggcg ctgctgaacg
     4501 atgtcgatgc ctgcaacgtc aactaaaacc acaaagcttg cgacgtcatt aatcgatgag
     4561 tacgccctgc tgggctggcg cgccatgctg actgaagtca acctgtcacc gaaaccaggc
     4621 ctcgtggatc ggattaactg cggtgcgcac aaagatatgg cgctggaaga tttccaccgc
     4681 agcgcgctgg cgattcaggg ctggttaccc cgtttcattg aatttggtgc ctgtagtgca
     4741 gaaatggcac cggaagcggt actcaacgga ttacgcccaa ttggtatggc ttgcgaaggt
     4801 gatatgttcc gcgccactgc gggcgtaaac acgcataaag gaagcatttt ttctttaggg
     4861 ctactgtgtg cggcaattgg ccgtttgctt cagctcaacc agccggtaac gccaacaacc
     4921 gtttgttcta cggcggcaag tttctgccgt ggcctgaccg atcgcgaact gcgtaccaat
     4981 aattcacgac tgacggcagg tcaacggttg taccagcagc ttggccttac cggcgcacgc
     5041 ggtgaagccg aagcgggtta tccactggtg atcaaccacg ccttgccgca ttacctcact
     5101 ctgctggatc aggggttaga tcctgagctg gcattgctcg ataccttgct cctactgatg
     5161 gctaccaacg gcgataccaa cgttgcatcg cgcggtggcg aggggggcct gcgctggcta
     5221 cagcgcgagg cgcaaacatt attgcaaaaa gggggcattc gaacccccgc cgatctcgat
     5281 tatctccggc agttcgacag ggagtgtatc gaacgaaatc tcagtccagg cggcagtgcc
     5341 gacctgctga tccttacctg gtttttagca cagatttaat tatttaagca cttgataaat
     5401 ttggaaatat taattttcgg agaacccgta tgtctttagc aaaagataat atatggaaat
     5461 tattggcgcc cctggtggtg atgggtgtca tgtttcttat ccctgtacct gacggtatgc
     5521 cgccgcaggc gtggcattac tttgcagtgt ttgtggcaat gattgtcggc atgatcctcg
     5581 agccaattcc ggcaacggcg atcagtttta ttgcggttac tatttgcgtt attggcagta
     5641 attacctgct ctttgatgcc aaagaattag ctgacccagc gtttaatgcg caaaaacagg
     5701 cgctgaaatg gggtctggct ggtttttcca gcaccacggt atggctggta tttggcgcat
     5761 ttatttttgc attagggtat gaagtttccg ggttaggccg tcgtattgcc cttttcctgg
     5821 tgaaattcat gggcaaacgc acgctgacgt tgggttatgc gattgtcatt atcgacattc
     5881 tgctggcacc gtttacaccg tccaacaccg cgcgtaccgg gggtacggtt tttccggtca
     5941 ttaaaaacct gccgccgctg tttaaatcat tcccgaacga tccgtccgcg cgtcgtattg
     6001 gcggctattt gatgtggatg atggtcatta gtaccagtct gagttcgtcc atgtttgtca
     6061 ccggtgcggc accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga
     6121 ttagctggtt gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg
     6181 cgccgtggct ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa
     6241 cctgggcggg tgatgaacta aaaaccatgg gtgcgctgac gcgcagagag tggacgctga
     6301 ttggccttgt attgctcagc ttaggtttat gggtatttgg cagtgaagtc attaatgcta
     6361 ctgcggtagg tctgctggca gtttcgctga tgctggcgct gcacgttgtg ccgtggaaag
     6421 acattactcg ctataacagc gcatggaaca cgctggtcaa cctggcaacg ctggttgtta
     6481 tggcgaatgg cctgacccgt tctggcttta tcgactggtt cgccaacacg atgagtacac
     6541 acctggaagg cttctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg
     6601 cacactacct gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg
     6661 ccgtcggtaa agggattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtactgt
     6721 ctatcggtat catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg
     6781 gctgtggcta tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt
     6841 acatctctat gctgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc
     6901 gccaaacgct gaataaatca tcacgcccgc caggtaagcc caccaggcgg gcttttttat
     6961 gatttaatag ataatattca gtgtatgtat gagttccaca cccattatga aagcattctg
     7021 gcgtaacgcc gcgttgctcg cggtttctct gcttcccttc tcttctgcca acgccgtggc
     7081 gttgcaggca aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac
     7141 cggattttgc cagagtcaac acgatcgaaa tcgtaacgaa cgagatgagt gtcgcctgca
     7201 aaccgaaacg accaacaaag ctgatttttt gaccgtacat ggtctgtggc caggattgcc
     7261 taaatcggtt gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac
     7321 tcgcccaatc ccgaatctgc cagaagcacg cgccagccgg atgtgttcat cgccggaaac
     7381 tgggttatca ctggaaacgg cagctaaact aagtgaagtc atgccaggag ctggcggacg
     7441 ttcctgcctg gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccaga
     7501 cgcatacttc ggcacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagctggaaa
     7561 attccttgcg gataactacg gtaacacagt gagccgccgt gactttgatg ccgcctttgc
     7621 caaaagctgg gtaaaagaga atgtgaaagc agttaagcta acgtgccagg gtaaccctgc
     7681 gtatttgact gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc
     7741 caactcattc ttgccacaac cacacccagg caactgtggc aaaacctttg tgattgataa
     7801 agcaggttat taatcctctg atttacaaaa cacgctaatt cgtcttccat aaaccatgac
     7861 gattaagatt tatacataat gcgctacatt gaggaattcc ctgaatgtga cgcaaatcac
     7921 ttcaaagagt gagcaacagt cagtatcatc atgtgagtta aaccctcgcc gcctgacggt
     7981 gagggttttc ttttgggtat gtttatctgc gacactcgca gtaccgacga catggagtaa
     8041 aaatgtccag accaactatc atcattaacg acctggatgc cgaacgcatc gatattctgc
     8101 tggagcaacc cgcctatgct ggtttgccaa tcgccgacgc gttaaacgca gagttggatc
     8161 gcgcccaaat gtgttcgcca gaagagatgc cacacgacgt ggtgacaatg aacagccggg
     8221 ttaaattccg caatcttagc gatggcgaag tgcgtgtgcg cacgctggtg tatccggcaa
     8281 aaatgaccga cagcaatact cagctttccg ttatggctcc ggtaggtgcc gcactgctgg
     8341 ggctgcgcgt tggcgattcc attcactggg aacttccggg cggcgttgca acccaccttg
     8401 aagtgctgga actcgaatac cagccagaag ctgctggcga ctacctgctt taatcccatc
     8461 tgcacaagcg acaactcgct ttgcccagag gatgcactag catcctcttt ccgctcaaat
     8521 atcaaccaga tttatcattt tgcgcttaag cgcagcattc taatctgaac tggttattca
     8581 tctgcggaac ctcatatgag cgaagctctc ggtatcactg cattttatat attcatagca
     8641 gcaattattg ttgctgcagt gctatacctt gaacaacgct ggtagcgcct ggttttattt
     8701 attttccagc tcaatagcaa attcagatca aatcagcctc gcaaattata gattcataac
     8761 caataatgtg ctgttatgta tacatgcaaa aacaaggaga gaatgcttat gtacaaaaca
     8821 atcatcatgc cagtggatgt tttcgaaatg gaattgagcg acaaagcgat tcgccacgct
     8881 gaattcctcg cccaggatga cggagttatt catctacttc acgtactacc ggggtcagcc
     8941 agcctgagcc tgcaccgttt tgccgctgat gtacgtcgtt ttgaagagca tctgcaacat
     9001 gaagcagaag aacgtttaca aacgatggtc agccacttca ccatcgatcc ttcccgcatt
     9061 aagcaacatg tccgttttgg tagtgtgcgg gatgaagtta acgagttagc gaaagaactt
     9121 gatgcagatg tagtagtgat tggttcgcgt aacccatcaa tttcaactca tctgttgggt
     9181 tctaacgcct cgagcgtgat ccgccacgcc aatttgccgg tgctggttgt gcgttaattg
     9241 ccagaataag tctcccgccc tgcccggagc catccctgca gggcttttta atgcaataaa
     9301 aaagccgcca ggtttgaccc cggcggctaa gcaattacag gtgaatctta cttcttctta
     9361 tgcagttttg gtgcgaatca ggtagtcaaa agcactcaga gaggctttgg caccttcgcc
     9421 agtggcgatg atgatctgct tgtacggaac cgtcgtacag tcacccgctg cgaacacgcc
     9481 tttcacgttg gtttcgcatt tcgcatcaat gataatctcg cccatgcgat tacgttcgac
     9541 tgcgccttcc agccagttgg tgttcggcag cagaccaatc tggacgaaaa taccggccag
     9601 ttcgatgttg tgaatattgc cgctgacacg atcgcgatat tccagaccta cgactttgct
     9661 accgtcgcct ttcacttccg tggtttgcgc attcagaata atgtcgacgt ttttcaggct
     9721 gcgcagtttg tcctgcagaa cctggtcggc tttcatttct ggcgcaaatt ccagcagcgt
     9781 tacgtgctca acgatacccg ccaggtcaat tgccgcttcc acgccggagt taccgccgcc
     9841 gataaccgct acgcgtttgc ctttaaacag cgggccgtcg cagtgcgggc agtaggtcac
     9901 gcctttggtg cgatactgat cttcgccagg aacgttcatg ttgcgccatt ttgcaccagt
     9961 cgccacgata atgctgcgtg ctttcagtac cgcgccagaa gctgtttcaa tctgatgcag
    10021 gccgccttca accgctgccg ggatcagttt gctcgcgctc tggctgtcga tcacatcaac
    10081 gtcgtattca tcaacatgaa ctttcagcgc acctgccagt ttctggcctt cggtcttcgg
    10141 tacagaaatg tagttttcga tatcaacggt atcgaggatc tgaccaccaa aacgttcgcc
    10201 catcagaccg gtacggatgc ctttacgtgc ggagtaaatt gctgccgctg cacccgccgg
    10261 gccggaacca acgattaata cgtcataagc atcacgctta ttcagctctt ctgccgcacg
    10321 tttttccgca ccagtatcaa ttttggcaac gatttcagtc aacgtcatgc ggccctgacc
    10381 aaactctttc ccgtttacga acactgccgg aacgcccatc acgttgcgat cggtgatttc
    10441 gttctggaag gtgccgccgt caattgcagt gtgcttgatg cgcgggttca gtacgctcat
    10501 caggttcagc gcctgcacca cgtccgggca gttgtggcaa gagagcgagt aataggtttc
    10561 gaattcaaaa tcaccgtcaa tatggcgaat ctgctccagc agagactgcg cttctttcga
    10621 cggatgacca ccggtccaca gcaacgccag taccagcgag gtgaactcgt ggcccagcgg
    10681 ggagcctgca aaacgtggtc cctggttgga acctgggttg gtgatcagga aagacggctt
    10741 acgcaccggc aagctgttat cttctttaaa ggtgactttg tctgacagtt ctgcgatttc
    10801 agccaacagt tccttgattt ctgccgattt agcgctgtca tccagcgtgg caattaactc
    10861 aacaggcttg gtcaatttct caaggtaagc cttgagttga gttttcatat ttgtgtcgag
    10921 catgtttatc tcctgggctt taaacatcat catgcaagcg gcctctgcgg ctagctggat
    10981 gcagcttgca tcatggtgcg gggaagaaaa tcgggtgccg cagcacccga agaattaggt
    11041 gaatttccga agtatctcgc gccggagtta gtagcgcgaa agacaaagga aatttagatt
    11101 ttaccaacca ggtccagaga cggagccaga gttgcttcac cttctttcca tttagccggg
    11161 caaacttcac ctgggtgaga agctacgtac tgtgctgctt tgattttacg cagcaggtct
    11221 gacgcgtcac ggccaatgcc ttcagcggta acttcgattg cctggatgat accctgcggg
    11281 tcaacaacga aggtcgcacg gtcagccaga ccttcatctt cacgcatgtt gtcgaagtta
    11341 cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt tgattttagc gatggtttca
    11401 gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg tagatactgc gtatacgtct
    11461 acgcccagtt tctgcaattc ttcgtaatgg tcagcaacgt cacccagttc ggtcgggcat
    11521 acgaaagtaa agtcagccgg gtagaagaag aagacgctcc agcggccttc ggtatctttt
    11581 tcggtgattt cgatgaattc gccgtttttg aatgcctggt ttttaaaagg tttaattttg
    11641 gtgttaatca aggacatcta tacttcctcc gtgttttcga tgagatgtaa ggtaaccaat
    11701 ttttgccgat tcggctaatg cgtttccatt atcaaatcga taagttttac cttacaacct
    11761 tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga taaaaagtaa aatggccccc
    11821 tttccaggtg accgtgtaat ctgaactacc ctttggtatc ttcagcgcct gcctgcaggc
    11881 tgaggtgttg cccttataaa tgcaacaacg acatggatta caacaccctc ataaacaaag
    11941 ggcaatcacc tgatctaagc tcttacctat gacagtgata ggttatgcct tttactcgac
    12001 ttttgcactg actgaaaagg acaaattaat gttaaaaaag atacttttac tggccctgct
    12061 tcctgcaatc gccttcgcag aggaacttcc tgccccagta aaagcgattg aaaaacaggg
    12121 tattacaatc atcaaaacgt tcgatgcccc tggaggaatg aaaggttatc tcggtaagta
    12181 tcaggatatg ggcgtcacca tctacctgac tccagatggt aagcacgcta tctctggtta
    12241 catgtacaac gaaaaaggtg aaaacctgag taacacactt atcgaaaaag aaatttacgc
    12301 acccgccgga cgtgaaatgt ggcaacggat ggaacaatcc cactggctcc tcgacggtaa
    12361 aaaagacgcg ccggtcattg tctacgtctt cgccgatccg ttctgcccat attgtaaaca
    12421 gttctggcag caggctcgtc cgtgggtaga ttctggcaaa gtgcaattga gaacattgtt
    12481 ggttggggtt atcaagccag aaagcccggc gacagcagcg gcaattcttg cctccaaaga
    12541 tcccgcaaaa acctggcaac aatatgaagc ctctggtggc aagcttaagc taagcgtgcc
    12601 tgcaaacgta agcacagagc aaatgaaagt gttaagcgac aacgagaaac tcatggacga
    12661 tctgggggca aatgtcacac cagctatcta ttacatgagt aaggaaaata ccctgcaaca
    12721 agccgtcgga ttacccgatc ag
//
LOCUS       DADSZD010000128        12722 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.129, whole genome shotgun sequence.
ACCESSION   DADSZD010000128 DADSZD010000000
VERSION     DADSZD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.129"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            33..491
                     /locus_tag="J1Z28_003306"
     CDS             33..491
                     /locus_tag="J1Z28_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBA7718116.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            521..2293
                     /locus_tag="J1Z28_003307"
     CDS             521..2293
                     /locus_tag="J1Z28_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBA7718117.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            2286..4067
                     /locus_tag="J1Z28_003308"
     CDS             2286..4067
                     /locus_tag="J1Z28_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBA7718118.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIDVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            4248..4586
                     /gene="glnK"
                     /locus_tag="J1Z28_003309"
     CDS             4248..4586
                     /gene="glnK"
                     /locus_tag="J1Z28_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBA7718119.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            4616..5902
                     /gene="amtB"
                     /locus_tag="J1Z28_003310"
     CDS             4616..5902
                     /gene="amtB"
                     /locus_tag="J1Z28_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBA7718120.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(5951..6811)
                     /gene="tesB"
                     /locus_tag="J1Z28_003311"
     CDS             complement(5951..6811)
                     /gene="tesB"
                     /locus_tag="J1Z28_003311"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBA7718121.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVSAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            7029..7601
                     /locus_tag="J1Z28_003312"
     CDS             7029..7601
                     /locus_tag="J1Z28_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718122.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(7632..7943)
                     /locus_tag="J1Z28_003313"
     CDS             complement(7632..7943)
                     /locus_tag="J1Z28_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBA7718123.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            8106..8202
                     /gene="ffs"
                     /locus_tag="J1Z28_003314"
     ncRNA           8106..8202
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="J1Z28_003314"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            8322..8675
                     /locus_tag="J1Z28_003315"
     CDS             8322..8675
                     /locus_tag="J1Z28_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBA7718124.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(8717..10267)
                     /gene="pdeB"
                     /locus_tag="J1Z28_003316"
     CDS             complement(8717..10267)
                     /gene="pdeB"
                     /locus_tag="J1Z28_003316"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBA7718125.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELNTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPSS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIIARLQKTPGEHIENNGIIYDILP
                     LPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHSQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(10431..10901)
                     /locus_tag="J1Z28_003317"
     CDS             complement(10431..10901)
                     /locus_tag="J1Z28_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718126.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(11017..11568)
                     /gene="maa"
                     /locus_tag="J1Z28_003318"
     CDS             complement(11017..11568)
                     /gene="maa"
                     /locus_tag="J1Z28_003318"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBA7718127.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLRAHRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPATIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(11741..11959)
                     /gene="hha"
                     /locus_tag="J1Z28_003319"
     CDS             complement(11741..11959)
                     /gene="hha"
                     /locus_tag="J1Z28_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBA7718128.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(11985..12359)
                     /gene="tomB"
                     /locus_tag="J1Z28_003320"
     CDS             complement(11985..12359)
                     /gene="tomB"
                     /locus_tag="J1Z28_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBA7718129.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         3042 a         3043 c         3438 g         3199 t
ORIGIN      
        1 taaaagagaa aaaaattctc tgtggaaggg ctatgttaga taaaattgac cgtaagctgc
       61 tggccttgct acagcaggat tgcaccctct ctttgcaggc actggctgaa gccgttaatc
      121 tgacaaccac cccctgctgg aagcgtctga aacggctgga ggacgacggt atccttatcg
      181 gcaaagtcgc cctgctggat ccggaaaaaa taggtctcgg cctgaccgcc tttgtgctga
      241 taaaaacgca acatcacagc agcgaatggt attgccgctt tgtcacggtg gttaccgaaa
      301 tgccagaagt gctggggttc tggcgcatgg ctggtgaata cgattatctg atgcgcgtcc
      361 aggttgccga catgaaacgc tacgacgagt tttataagcg tctggtaaac agtgtaccgg
      421 ggctgtcgga cgtcacttcc agcttcgcga tggaacagat taaatacacc acttctttac
      481 ccatcgaata aatatccaga atcaagtcag gacacaacgc gtgcgattat ttgctcaatt
      541 aagctggtat ttccgtcggg aatggcgtcg ctatctcggg gctgtcgcct tgcttgtcat
      601 tatcgcgatg ctgcaactgg ttccgccaaa agtggttggt attgttgttg atggcgtgac
      661 agaacaacac tttactaccg ggcagatcct gatgtggatc gccaccatgg tgctgattgc
      721 cgttgtggtt tatctcctgc gttacgtctg gcgcgtattg ctgtttggtg cgtcttatca
      781 actggctgtt gaactgcgtg aagattatta ccgccagcta agccggcagc atcctgagtt
      841 ttacctgcgt catcgcacgg gtgacctcat ggctcgtgcg accaatgacg tcgatcgcgt
      901 cgtgtttgcc gccggagaag gggtgctgac gctggtggat tcactggtaa tgggctgcgc
      961 tgtgttgatt atgatgtcta cgcaaattag ctggcagttg accttatttg ccctgttgcc
     1021 gatgccagtg atggcgatca tgatcaagcg caacggcgat gccttgcatg aacgctttaa
     1081 gttggcacag gcggcgtttt ccagtcttaa tgaccgcacc caggaaagcc tcaccagtat
     1141 ccgcatgatc aaagcctttg gtctggaaga tcgtcagtcg gcgttatttg ccgcggatgc
     1201 cgaagatacc ggcaaaaaaa acatgcgggt ggcgcgtatt gatgctcgtt tcgacccgac
     1261 catctatatc gcgattggta tggcgaactt gctggcgatt ggcggcggta gttggatggt
     1321 ggtacagggc agtttaacgc tgggccagct caccagtttt atgatgtatt taggtctgat
     1381 gatttggcca atgctggcgc tggcatggat gtttaacatt gtggaacgtg gcagcgcggc
     1441 gtatagccga attcgtacca tgctggccga agcgccagtc gttattgatg gtagtgataa
     1501 ggtgccagaa ggtcgtggtg aactggatgt gaatattcgc cagtttacgt atccgcagac
     1561 tgaccatcct gcgctggaaa acgtcaattt cgccctgaaa cccggtcaga tggtaggtat
     1621 ttgcgggccg actggttccg gcaaaagtac cctgttgtcg ctcattcagc gtcatttcga
     1681 cgtcagcgag ggggatatcc gctttcatga tattcctctg acgaagttac aactcgatag
     1741 ctggcgtagc cgcctggcgg tagttagcca gacaccgttc cttttttccg acaccgtagc
     1801 gaacaacatc gcgttgggtt gcccgaatgc cacccagcaa gagattgagc atgtcgcgcg
     1861 gttagccagc gtgcatgacg atattttgcg tttaccgcaa ggttacgata cagaagtggg
     1921 cgagcgcggc gtgatgcttt ccggtgggca aaaacagcgt atctccattg ctcgtgcgtt
     1981 attagtcaac gcggaaatcc tcatccttga tgatgcgctt tcggcagtag acggacgcac
     2041 agagcaccag atcctgcata acctgcgtca gtgggggcag ggaagaacgg taatcatcag
     2101 tgcccatcgc ctttctgcac tgacggaagc cagtgaaatt attgtgatgc agcacggaca
     2161 tatcgcccag cgtggcaatc atgatgagct ggcgcaacaa agtggctggt atcgcgatat
     2221 gtatcgctat caacaactgg aggcggcgct cgacgacgct ccggaaattc gcgaggaggc
     2281 catcgatgcg tagttttagc caactgtggc cgactctcaa gcgcctgtta gcgtacggtt
     2341 caccgtggcg taaaccgttg gggattgcgg tcctgatgat gtgggttgcg gcggcggcag
     2401 aagtcagtgg gccgctgctt atcagctatt ttatcgacaa tatggtagcg aaaaataacc
     2461 tgccgttgaa agtggttgca gggctggcgg cggcgtatgt tgggctgcaa ctgtttgcag
     2521 ccgggctaca ttacgcgcag tcgctgctgt ttaatcgggc ggcagtaggc gtagtgcaac
     2581 agttgcgtac cgacgtgatg gatgctgcgt tacgccagcc attaagcgag tttgataccc
     2641 aacccgtcgg gcaggtgatt tcccgcgtca ctaatgacac tgaagtgatc cgcgatctct
     2701 acgttaccgt agtggcaact gtcctgcgta gcgccgcact ggtgggcgcg atgctggtgg
     2761 cgatgttcag cctcgactgg cgaatggcgc tggtggcgat aatgattttc ccggtggtgc
     2821 tggtggtaat ggtgatatat cagcgttaca gcacgccgat tgtccgtcgt gtgcgcgctt
     2881 atctggcgga tatcaacgac ggctttaacg aaatcatcaa tggcatgagc gttatccagc
     2941 agttccgtca gcaggcgcga tttggcgaac gtatgggtga ggccagtcgt tcacactata
     3001 tggcgaggat gcaaaccctg cgcctcgacg gttttctgct gcgtccgctg ctgagtctgt
     3061 tttcatcgct cattctttgt ggcttgttga tgctgtttgg cttctccgcc agcggcacca
     3121 ttgaagtggg cgtgttgtat gcgttcatca gttatttggg gcgacttaac gaacccttaa
     3181 tcgaactgac cacgcagcag gcgatgctgc aacaggctgt tgttgctggt gagcgcgttt
     3241 ttgagctgat ggacggaccg cgccagcaat atggcaatga tgatcgcccg ttgcagagcg
     3301 gcaccatcga cgtcgataac gtgtcatttg cttatcgcga tgacaatctg gtgctaaaga
     3361 acattaatct ctctgtgcct tcgcgcaact ttgtggcgct ggtcgggcat accggcagtg
     3421 gcaaaagcac cctcgccagt ttattgatgg gctattaccc gctaacggaa ggtgagattc
     3481 gccttgatgg tcgtccatta agtacgctaa gtcacagcgc gctgcgccag ggcgtggcaa
     3541 tggtgcagca agatccggtg gtgctggcgg ataccttcct cgccaacgtg acgctgggac
     3601 gtgatatctc tgaagaacgc gtctggcagg cgctggaaat cgtgcagctg gcggagctgg
     3661 cgcgtagcat gagtgatggt atttacacgc cgcttggcga gcaggggaat aatctctcag
     3721 tcgggcaaaa gcaactgctg gcactggcgc gcgtgctggt ggagacgccg caaatcctga
     3781 tccttgatga ggcaaccgcc agcattgact ccgggactga acaggcgatt caacatgctc
     3841 tggcggcggt gcgtgaacat actacgcttg tggtgattgc tcaccgctta tcgactattg
     3901 ttgatgccga caccattctg gtgcttcatc gtgggcaagc cgtggagcag ggcactcacc
     3961 agcaactgct ggcggcccag ggacgatact ggcagatgta tcaactgcaa cttgcgggcg
     4021 aagagctggc agccagcgtg cgtgaagagg aatcattgag cgcctgaata gcgtaatatt
     4081 tcatcgttgg tgcaaaaatg taacgcactg tgcactgtca tagtgcgttt tcattttcaa
     4141 tcttcttaac ttcctgctct ctttctcgtt tttcatttct ggcacaccgc ttgcaatacc
     4201 ttcttcgtgt agcagaacca ttaccgaatt ctgaccggag gggatctatg aagctggtga
     4261 ccgtgataat caaaccattc aagctggaag acgttcgtga agcgttatct tccattggta
     4321 ttcagggcct gaccgtcacc gaagtgaaag gtttcgggcg tcagaaaggg catgccgagc
     4381 tgtaccgggg ggcggaatac agcgtcaact tcctgccaaa agtaaaaatt gatgtggcga
     4441 ttgctgatga ccaactcgat gaagtgatcg atatcgtcag caaagcggct tacaccggaa
     4501 aaattggcga cggcaaaatc ttcgtcgctg aattgcaacg cgtcattcgt attcgtaccg
     4561 gcgaagccga cgaagcggcg ctgtaatctc tggcacacag caacaggaac gaaaaatgaa
     4621 gatagcaacg ataaaaactg ggcttacttc actggcgatg ctgccgggac tggtaatggc
     4681 tgcacctgcg gtggccgata aagccgacaa tgcgtttatg atgatttgta ctgcgctggt
     4741 gctgtttatg accgttccgg ggattgccct gttttatgga gggctgattc gtggcaaaaa
     4801 cgtgctgtcg atgctgacgc aggtgaccgt gacgtttgca ctggtctgta ttctctgggt
     4861 ggtttacggc tactcgctgg cgtttggcga aggcaacaac ttcttcggca acattaacgg
     4921 gttgatgctg aaaaacattg aactgacggc ggtgatgggc agcatttatc agtatatcca
     4981 cgtggcgttt cagggatcgt ttgcctgcat taccgtaggc ttgatagttg gggcgctggc
     5041 ggaacgaatc cgcttctcag ctgtgttgat ttttgtggtg gtatggctga cgctctctta
     5101 cattccgatt gcgcatatgg tctggggcgg cggtttgctg gcttctcacg gtgcactgga
     5161 ttttgcgggt ggcacggtgg tgcacattaa cgccgcaatc gccggtctgg tgggcgcgta
     5221 tctgataggc aaacgcgtgg gcttcggcaa agaggcgttt aaaccgcaca acctgccgat
     5281 ggtcttcacc gggactgcca ttctctatat cggttggttt ggctttaacg ccgggtcagc
     5341 gggcacggcg aatgaaatcg cggcactggc atttgtgaat actgtagtcg caactgcggc
     5401 ggcaattctt ggctggatct tcggtgaatg ggcgctgcgt ggtaagcctt cactgctggg
     5461 agcgtgttct ggcgcgattg ccggtctggt cggcgtgacg ccagcctgtg gttacattgg
     5521 ggttggcggc gcgttgatta tcggcgtggt agctggtctg gcgggcttgt ggggcgttac
     5581 catgctcaaa cgcttgctgc gggtggatga tccctgcgat gtcttcggtg tgcacggcgt
     5641 ttgtggtatt gtcggctgta tcatgaccgg gatttttgcc gccagctcgc tgggcggcgt
     5701 gggcttcgct gaaggtgtga cgatgggcca tcagttgctg gtacagctgg aaagcatcgc
     5761 cattacgatt gtctggtctg gtgtggtggc atttatcggc tacaaactgg cggatttgac
     5821 ggttggtctg cgtgtaccgg aagagcagga gcgagaaggg ctggacgtca acagccacgg
     5881 cgagaatgcc tataacgcgt aacaagcact gcaaaaaaca gccggacggt tttcacctcc
     5941 ggctattttt ttaattgtga ttacgcatca ccccttcctg aacggtcgag gcaaccagca
     6001 cgccgtcctg ggtataaaac tcaccgcgca caaagccgcg tgcgctggac gccgaggtgc
     6061 tttccacgct atacagcagc cattcattca aattaaacgg gcgatggaac cacatggaat
     6121 ggtcaatggt ggcaatctga atccccggtt cgagaaaacc gatgccgtgc ggctgtagag
     6181 ctaccggcag gaaattaaga tcagaagcat aaccgagcag atactgatga acgcgcagat
     6241 catccggcac gctaccattt gcgcggatcc acacctgacg atgcggttct gcgacgtgac
     6301 ctttcagtgg gttatgaaac tccaccggac ggacttccag cggacgatcg cagatgaatt
     6361 tatctttcag cactggcggc agcaggtgcg ccagcgattg ggcgatttgc gtttctgaag
     6421 gcaggccatc aggcgctggc gcggacggca ttgttttttg atgttcgaaa cccgcttctg
     6481 gtgcctggaa agaggcagtc atataaaaaa tcggtttgcc gttttgaata gcagaaaccc
     6541 ggcgggcgct gaagctgtta ccgtcacgca gcgtttcgac atcataaata atcggcttct
     6601 tactatcgcc agggcgaaga aagtagctgt gaaaagaatg taccagccgc tcgtcaggga
     6661 cggtctcttt tgcagcatac aaggcctgac ccacgacctg gccgccaaat acctggcgta
     6721 aacctaaatc ttcactctgg ccgcgaaaga gtccttcctc aattttttcc agatttaaca
     6781 atgtcagtaa attttttagc gcctgactca tataactctc cagtaacaaa gctgccgcag
     6841 caagccaaag tgagttgagt ataacgcaaa tttgccactg gtccgatggg tgcaatggtc
     6901 tgaattacgg gctaattaca ggcagaaatg cgtgatatgt gccacacttg ttgacgttac
     6961 tattttgtta accactcttc cggcgaggaa agttagcccg ctggtgcatt gataataagg
     7021 agaagtgaat gaaactcgtg cacatggcca gtggtttagc ggttgcgatt gcgttggcgg
     7081 cttgcgcaga taaaagcgcg gatattcaga cgccagcacc ggctgcaaat acgtctattt
     7141 cagcaacaca acaacctgct atccagcaac cgaatgtctc cggtaccgtt tggatccgtc
     7201 agaaagtcgc actgccgcct gatgccgtgc tgaccgtgac actttctgac gcgtcgttag
     7261 ccgatgcgcc gtcgaaagtg ctggcgcaga aagcggtgcg taccgaaggt aaacagtcac
     7321 cattcagctt tgttctgcca tttaacccgg cagatgttca gccgaacgcg cgtattctgt
     7381 tgagtgcggc gattaccgtg aatgacaaac tggtatttat taccgatacc gttcagccgg
     7441 tgatcaacca gggcggaact aaagccgacc tgacattggt gccggtgcag caaaccgccg
     7501 tgccggttca ggctagcggt ggcgcaacga ctaccgtacc ttcaacttca ccaacccagg
     7561 tgaatccgtc ttcggcagtt ccagctccaa cgcaatatta agcgaagtta accctctccg
     7621 cccggagagg gttagtagtt ccagcgataa agctgcaagt cgatttgccc gcttcccgac
     7681 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg tccggtcagt
     7741 gaaatggtgc cgtggcgatt aaccacccgg tgccagggca aggtgctgcc ttcggggaga
     7801 cgctttaaca caccgcctac ctggcgcgcg gcgcgcggcg atcccgccag tttcgccaca
     7861 tcaccgtaag tggtgacata gccttcggga atagcggcta cgatttgcca gacgcgttgg
     7921 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
     7981 aaaacgcccg agtgaagccg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
     8041 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttctcaacgc tctcaatggg
     8101 ggctctgttg gttctcccgc aacgctactc tgtttaccag gtcaggtccg gaaggaagca
     8161 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
     8221 tcccaccgtt tcgtcaaaaa atcccaacat ggctaaactt taaccacaac tgacgtcgca
     8281 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
     8341 ttgttgttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
     8401 cgccattatt taaagagttc ggtgcgcttc gtattgtcga atgctgggcc agcgatgtac
     8461 cggatggtaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
     8521 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgctgctaat caaaagatga
     8581 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     8641 tctatggtgg attcgaatca atcatcgacg agtaggtagc gtgacgggct gtgcggcgtg
     8701 ccgtcagccc taaaattcac aaatgttgct cggcccagcg taagaaatcc tctttcggta
     8761 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     8821 cttgtttact ggtttcgatt ccctccgcaa ccattttcag tttcagtgtt tttgccattt
     8881 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     8941 tcagaatgtc gacatccaga tcctgtaaat agcttaaact tgaataccct gtgccaaaat
     9001 catcgagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     9061 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     9121 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     9181 caagattgat cgaaatatgc tgctgagaat gctgacgcag ccagtcgccc atatcttcaa
     9241 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     9301 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     9361 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     9421 cacgattttc gatagcatcc tgcagccgat gatgcggtga ctggatacgg cgcaaaatac
     9481 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     9541 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     9601 tcatctccgg taagggcagg atatcgtaaa tgattccatt attttcgata tgctcaccgg
     9661 gtgttttttg tagcctggca ataattccct gagcaatttt atcgctgctg gttatgacaa
     9721 cgttatgggc attgccaata atggcggcat caatttgcca tgagctatag ggaatgacat
     9781 caataaagga agaggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     9841 aacgaataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     9901 ttttacctgg ctcggggaag gtatcgggcg ggctttcatg ctccagagac gaacattggg
     9961 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
    10021 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcttgca
    10081 gcgcatcttt cccttgtgtc gccaccttat tggcccgaat agcgacgcgg gaggaatacg
    10141 tattcagctc ttcaataaat gatgtttcta cctgctgatg ggccagccag atgcttaagc
    10201 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
    10261 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
    10321 ggctggcagg agataaggag gtagagccgg gatttcatga agggatgggt ggaaacgaac
    10381 gttgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
    10441 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
    10501 attttttgca gttgttcttt atgaacatcc gcgacacgcg gagagttcag gatggcttca
    10561 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
    10621 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
    10681 ttaagcagga taaataatac aaccagcagc cagacagagc ctgaaagcgt ttcggattgc
    10741 aacatcaccg ccagggtagc aaaaaaagca acatacatac cgataaacag ccccggatgt
    10801 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
    10861 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtgatatc
    10921 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
    10981 ttgccacgat tttgcataac agttgagaaa agctggttac aattttttaa tgattctggc
    11041 tggattaccg cccacgacaa cgttgtccgg gacatctttt gtgacaactg cacctgaggc
    11101 taccacgacg ttatcaccaa tggtcacacc agggttaatg actgcacgtc cgccaatcca
    11161 gacgttatta ccgatggtgg cgggtttccc cagttcagca ccgctattac gtgctacagg
    11221 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
    11281 aatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
    11341 gagaaaaatg ttatagccat agtcacagcg aaacgttggc tcaatataag cctctgtcac
    11401 ctgaccgaat aaatcagcaa gaatttgctg gcgtaatgtg tgctcttccg ccagggaatg
    11461 attgtatcgg tgaataagcc gatgagcgcg caggcgatcg cgagataacg cctcatctgc
    11521 cgagtgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
    11581 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttcccatta
    11641 catctgtgtg atgaagatca catagggtat gtcttcatgg cgaaaaaagt gcaaaattct
    11701 taataaacag ccagttatag ctccgaaagc gagttattta ttagcgaata aatttccata
    11761 ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa ttcggcgagg cggtgatctg
    11821 cggctgagta aaataccgcc agttcattat ctgataattc gtatttattt ttctcgataa
    11881 cacgctccag cgtgtcaatt gtctggcaac gacgtaaacg cattaaataa tcggttttcg
    11941 ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca agataaactc
    12001 gcaggattct ctttcgtcgc attgacaaaa caacggaata gtcgattacc tgacttccgc
    12061 catttctgaa gatcctgcat attaatacca taactactga acaacataaa ggtgtcatcc
    12121 agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat tttgtaatta
    12181 agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact ggaggttgat cgccgaggtt
    12241 gggtcgttaa cccagccatg attgctttct tcaaggtttg caaggcagtc atgatacagg
    12301 gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta ttcatccata
    12361 acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt cgggatggaa aaaacttact
    12421 gacctggact tgccctctcg tttgttagct taactataag ccactctttg taggttttca
    12481 tcgcattatt acgaaaaatt acaattagga aggaagcggg acaggactta ctttttcata
    12541 ctagcaccaa cccgcagcag gtaaaagcag tttgctcgaa ctgttcataa catttcagaa
    12601 ttatcgccag aagaaatcgg tgacactaat accaggattg ctctgaatat gacgtaataa
    12661 ccgaggaatg aataaagaat taccgcaaat aattaagaat agcctctaaa tggttatgga
    12721 tt
//
LOCUS       DADSZD010000129        12564 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.130, whole genome shotgun sequence.
ACCESSION   DADSZD010000129 DADSZD010000000
VERSION     DADSZD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.130"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            8..1522
                     /locus_tag="J1Z28_003321"
     CDS             8..1522
                     /locus_tag="J1Z28_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HBA7718130.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSVLVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1552..2694
                     /gene="caiA"
                     /locus_tag="J1Z28_003322"
     CDS             1552..2694
                     /gene="caiA"
                     /locus_tag="J1Z28_003322"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBA7718131.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2823..4040
                     /gene="caiB"
                     /locus_tag="J1Z28_003323"
     CDS             2823..4040
                     /gene="caiB"
                     /locus_tag="J1Z28_003323"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBA7718132.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4113..5666
                     /gene="caiC"
                     /locus_tag="J1Z28_003324"
     CDS             4113..5666
                     /gene="caiC"
                     /locus_tag="J1Z28_003324"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBA7718133.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASELRHICLTDMALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5775..6560
                     /gene="caiD"
                     /locus_tag="J1Z28_003325"
     CDS             5775..6560
                     /gene="caiD"
                     /locus_tag="J1Z28_003325"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBA7718134.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6566..7156
                     /gene="caiE"
                     /locus_tag="J1Z28_003326"
     CDS             6566..7156
                     /gene="caiE"
                     /locus_tag="J1Z28_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBA7718135.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVERCHAALHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7275..7670)
                     /gene="caiF"
                     /locus_tag="J1Z28_003327"
     CDS             complement(7275..7670)
                     /gene="caiF"
                     /locus_tag="J1Z28_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBA7718136.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7931..11152)
                     /gene="carB"
                     /locus_tag="J1Z28_003328"
     CDS             complement(7931..11152)
                     /gene="carB"
                     /locus_tag="J1Z28_003328"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBA7718137.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11170..12318)
                     /gene="carA"
                     /locus_tag="J1Z28_003329"
     CDS             complement(11170..12318)
                     /gene="carA"
                     /locus_tag="J1Z28_003329"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBA7718138.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTIKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
BASE COUNT         2921 a         3277 c         3348 g         3018 t
ORIGIN      
        1 ttaactcatg aagaatgaaa agagaaaaac gggaatagaa ccgaaggttt tctttccgcc
       61 gttaataatc gtcggcatac tttgttggct tacagtcaga gatctggatg cggcgaatgt
      121 cgttattaat gctgtattca gttacgtcac caatgtatgg ggatgggcat ttgaatggta
      181 tatggtggtg atgcttttcg gctggttctg gctggtgttt ggcccgtatg ccaaaaagcg
      241 tttaggtaac gaaccaccag aatttagtac cgccagttgg atctttatga tgttcgcttc
      301 ctgtacgtct gctgccgtac tgttctgggg atcgattgag atctactact acatctccac
      361 cccgccgttt ggcttagaac cgaactcaac aggcgcgaaa gagttggggc tggcttacag
      421 cttgttccac tggggacctc tgccgtgggc cacttacagc ttcctttcag tcgccttcgc
      481 ttacttcttc tttgtccgca aaatggaagt gattcgcccc agctccaccc tggtgccgct
      541 ggtaggtgaa aaacacgcca aagggttgtt cggcactatc gtcgacaact tctacctcgt
      601 cgccttgatc ttcgcgatgg gtaccagtct gggccttgcc acgccgctgg tgaccgagtg
      661 tatgcaatgg ttgtttggca ttccgcatac cctgcaactg gacgctatca tcattacctg
      721 ctggattatc ctcaacgcca tttgcgtcgc ttgcggtctg caaaaagggg tacgtatcgc
      781 cagtgacgtg cgtagttacc tgagcttcct gatgctgggt tgggtgttca tcgtcagcgg
      841 tgccagcttc atcatgaact acttcaccga ttcggtgggg atgttgctga tgtatctgcc
      901 gcgcatgttg ttctataccg atcccatcgc taaaggcggc ttcccgcagg gctggaccgt
      961 gttctactgg gcatggtggg tgatttacgc tatccagatg agtatcttcc tcgcccgcat
     1021 ctcccgtggt cgtaccgtgc gtgaactgtg cttcggcatg gtgctggggc tgacagcgtc
     1081 aacctggatc ctgtggactg tactcggtag taacactctg ctgttgatag ataaaaacat
     1141 catcaacatt ccaaatctga tcgaacagta cggtgtggcg cgcgccatca tcgaaacctg
     1201 ggccgctctg ccactcagca ccgccaccat gtggggcttc ttcatcctct gctttattgc
     1261 caccgttacg ctggttaacg cctgctctta taccctggcg atgtccactt gccgcgaagt
     1321 acgcgatggt gaagaacccc cgctgctggt gcgtatcggc tggtcagttc tggttggcat
     1381 tatcggcatt gttctgctgg cgctcggcgg cctgaaaccg attcaaaccg ccattatcgc
     1441 cggaggatgc ccgctgttct tcgtcaacat catggtgacg ctctccttta ttaaagacgc
     1501 gaaacagaac tggaaagatt aattaacccc caaatatcaa gaggttgaaa gatggatttt
     1561 aatttaaatg atgagcagga actgtttgtc gccggtatcc gcgaactgat ggccagcgaa
     1621 aactgggagg cctattttgc cgagtgcgac cgtgacagcg tctacccgga acgttttgtc
     1681 aaagccctgg cggatatggg tatcgacagt ctgctgatcc cggaagaaca cggtggtctg
     1741 gacgcggggt ttgttactct cgccgccgtg tggatggaac tgggtcgtct gggagcgcca
     1801 acttacgtgc tgtatcagtt accgggcggg tttaacacct tcctgcgtga aggtacgcaa
     1861 gagcagatcg acaagatcat ggctttccgc ggcaccggta agcagatgtg gaactcagcg
     1921 attaccgaac cgggcgcagg ctccgacgtg ggtagcctga aaacgactta tacccgtaga
     1981 aatggtaaga tttatcttaa tggtagtaag tgttttatta ccagtagcgc ctacaccccg
     2041 tacatcgtgg tgatggcgcg cgacggggct tctccggaca aacctgtcta caccgaatgg
     2101 tttgttgata tgagcaaacc gggcatcaaa gtgaccaaac ttgaaaagct cggtctgcgt
     2161 atggatagct gctgtgaaat cacttttgac gacgtggaac tggacgagaa agacatgttc
     2221 ggtcgggaag gtaacggctt taaccgcgtc aaagaagagt tcgaccatga acgtttcctg
     2281 gtagccctga ccaactacgg tacggcgatg tgcgcctttg aagatgcggc gcgctacgcc
     2341 aaccagcgcg tgcagtttgg cgaggctatt ggtcgtttcc agttgattca ggaaaaattc
     2401 gcccacatgg cgatcaaatt aaactccatg aaaaacatgc tgtatgaagc agcgtggaaa
     2461 gcagacaacg gcaccatcac ctctggcgat gcagcgatgt gcaaatactt ctgcgccaat
     2521 gcggcatttg aagtggtgga tagcgcaatg caggtgctgg gcggtgtcgg gattgcgggc
     2581 aaccaccgca tcagccgctt ctggcgtgac ctgcgtgtag accgcgtttc cggaggatct
     2641 gacgaaatgc agatcctgac gctgggtcgc gcggtgctga agcaataccg ctaagtccgt
     2701 gcgctgcctg atgcgacgct gccgcgtctt atcaggccta cgggagcacg aatgtcggcc
     2761 ggataaggcg tttacgccgc atccggcagt catgcgctac ataaattttt caggagctaa
     2821 taatggatca tctacccatg ccgaaattcg ggccgctggc cggattgcgc gttgtcttct
     2881 ccggtatcga aatcgccgga ccgtttgccg ggcaaatgtt cgcagaatgg ggggcggaag
     2941 ttatctggat cgagaacgtc gcctgggccg acaccattcg cgttcaaccg aactacccgc
     3001 agctctcccg ccgcaatttg catgcgctgt cgttaaatat tttcaaagat gaagggcgcg
     3061 aagcgtttct gaaattaatg gaaaccaccg atatcttcat cgaagccagt aaaggtccgg
     3121 cctttgcccg tcgtggcatt accgatgaag tcctgtggca gcacaaccca aaactggtta
     3181 tcgctcatct gtccggtttt ggtcagtacg gcaccgagga gtacaccaat cttccggcct
     3241 ataacactat cgcccaggcc ttcagtggtt acctgattca gaacggtgat gttgaccagc
     3301 caatgcctgc cttcccgtat accgccgatt acttttctgg cctgactgcc accacggcgg
     3361 cgctggcagc actgcataaa gtgcgtgaaa ccggcaaagg cgaaagcatc gacatcgcca
     3421 tgtatgaagt gatgctgcgt atgggccagt acttcatgat ggattacttc aacggcggcg
     3481 aaatgtgccc gcgcatgacc aaaggtaaag atccctacta cgccggttgc ggtctgtata
     3541 aatgcgccga cggctacatc gtgatggaac tggtgggcat tactcaaatt gcagagtgct
     3601 ttaaagatat tggcctcgca catctgctcg gtacgccgga aatcccggaa ggcactcagc
     3661 ttatccaccg tatcgaatgc ccttacggcc cactggtaga agagaaactc gatgcctggc
     3721 tggcggcaca taccatcgca gaagttaaag aacgctttgc cgaactgaat atcgcctgtg
     3781 ccaaagtact gaccgtaccg gaactggaaa gcaatccaca gtatgtggct cgcgaatcaa
     3841 tcactcagtg gcaaacgatg gatggtcgca cctgcaaagg gccgaatatc atgccgaaat
     3901 tcaaaaataa ccccggacaa atctggcgcg gaatgccctc acatggcatg gacacggctg
     3961 ctattttgaa aaatatcggc tacagcgaaa acgacattca ggagttggtc agcaaaggtc
     4021 tggccaaagt tgaggactaa acgcgtggcg tcagtcagct tgcctgacta gcgcccgctc
     4081 aataacaaac gaacaaaatg gatagaggtg caatggatat cattggcgga caacatctac
     4141 gtcaaatgtg ggacgatctg gcggacgttt acggtcataa aacggcgctg atttgtgaat
     4201 ccagcagcgg agtcgttaac cggtatagtt atcttgagtt aaatcaggag attaaccgca
     4261 cggcaaacct gttttatgtg ctggggattc gcaaaggcga caaggttgct ctacatctcg
     4321 acaactgccc ggaatttatc ttttgctggt tcgggttggc aaaaattggc gcgattatgg
     4381 tgccgattaa cgcccgcctg ttgcgcgaag aaagcacgtg gatcctgcaa aatagccagg
     4441 cgtgcctgct ggtgaccagt gcgcaatttt atcctatgta tcaacagatt cagcaggaag
     4501 atgcctctga gttgcggcac atttgcctga cagatatggc acttcccgct gatgatggcg
     4561 tgagttcgtt tactcaattg aaaaatcaac aacctgccac cttgtgctat gcaccgccgc
     4621 tatcgactga cgatacggcg gaaattctct tcacctccgg caccacctcc cgaccgaaag
     4681 gggtggtgat aactcattac aacctgcgct tcgctggata ttactccgcc tggcagtgtg
     4741 cactgcgtga cgatgacgtc tacctgacgg taatgcctgc gtttcatatc gattgccagt
     4801 gtactgcggc gatggcggcg ttttctgccg gggccacctt tgtgctggtc gagaaataca
     4861 gcgcccgtgc cttctgggga caggtgcaga agtaccgcgc caccattacc gaatgtattc
     4921 cgatgatgat ccgtacgttg atggtgcagc cgccttcagc caacgatcgg caacactgcc
     4981 tgcgggaagt gatgttttat ctcaacttgt cggagcagga aaaagacgcg ttttgtgaac
     5041 gcttcggcgt tcgcttgctg acgtcttatg ggatgacgga aaccattgtt ggcattatcg
     5101 gcgatcgccc tggcgataaa cgacgctggc cgtcgattgg tcgggcgggg ttttgctacg
     5161 aagcggagat ccgcgacgat cacaatcgcc cgctcccggc aggtgaaatc ggtgaaatct
     5221 gtattaaagg cgtaccaggg aaaaccatct tcaaagagta ttttctcaac ccgaaagcca
     5281 ctgcgaaagt gctggaagcc gatggctggc tacataccgg cgataccgga taccgcgacg
     5341 aagagggctt tttttatttc gtcgatcgcc gctgcaatat gatcaaacgc ggtggcgaga
     5401 atgtctcctg cgtggagctc gaaaatatca tcgccaccca tcccaaaatt caggatatcg
     5461 tggttgtggg tattaaagat tcgattcgcg atgaagccat caaagcattt gtggtgctga
     5521 atgaaggtga aacattgagc gaagaggaat tcttctgctt ctgtgaacag aatatggcga
     5581 aatttaaagt gccctcttat ctggagatca gaaaagatct gccacgtaat tgctcgggga
     5641 aaataattag aaagaatctg aaataaatga aacggcaggg aactaccctg ccagctaata
     5701 atcacgctct aaaacaatat gcattttttg cagggatgtt gtcgtccctg aaaaagcaaa
     5761 aatggagaaa aggaatgagt gaatcattac atctgacccg caatggatca attctggaaa
     5821 ttacccttga tcgtccaaaa gcgaatgcta ttgatgcaaa aaccagcttt gaaatgggcg
     5881 aagtatttct aaatttccgt gacgatccgc aattacgtgt cgccattatt accggtgccg
     5941 gagagaaatt cttttccgcg ggctgggatt taaaagcggc agcagaaggc gaagcaccgg
     6001 atgctgactt tggcccggga ggttttgcgg gattaaccga aattttcaat ctcgacaaac
     6061 cggtcatcgc cgctgtgaac ggctatgcct ttggcggcgg ctttgaactg gcgctggcgg
     6121 cagattttat tgtttgtgcc gataacgcca gcttcgccct accggaagcc aaactgggca
     6181 tcgttcctga cagcggcggc gtgctgcgtc tgccgaagat cctgccgcct gccatcgtca
     6241 atgaaatggt gatgaccggc agacgaatgg gcgcagaaga ggcgctgcgt tggggggtag
     6301 tcaaccgcgt ggttagccag gcggaactga tggataacgc ccgcgaactg gcgcagcagc
     6361 tggttaacag cgctccgctg gcgattgcgg cgctgaaaga gatctaccgc accaccagtg
     6421 aaatgccggt agaagaagcg tatcgctata ttcgcagcgg cgtgttgaaa cactatccgt
     6481 cggttctgca ttcggaggat gccattgaag ggccgctggc gtttgccgag aagcgcgatc
     6541 cagtgtggaa aggacgttaa caaccgtgag ctattacgcc tttgaggggt taattccggt
     6601 tgttcacccg acggcgtttg tccatcccag tgcagtattg attggcgatg tgattgtggg
     6661 agccggtgtc tacatcggcc cactcgcctc actgcgtggt gactacgggc ggttgatcgt
     6721 gcaagcggga gccaatattc aggatggctg cattatgcat ggctactgcg acactgacac
     6781 catcgttggg gaaaacggtc atatcgggca cggagcgatc ctgcatggtt gtgtgattgg
     6841 tcgcgatgca ctggtcggga tgaacagcgt gattatggat ggcgcggtca ttggcgaaga
     6901 gagcattgtt gccgctatga gctttgtcaa agcgggcttt cgcggcgaga aacgccagtt
     6961 gttgatgggt acgccagccc gcgctgtacg cagcgtgagt gacgacgagt tacactggaa
     7021 acggttgaat accaaagagt atcaggatct tgtcgagcgc tgtcatgcag cgttacatga
     7081 aacgcagcca ctgaggcaaa tggaggaaaa tcgcccccgt ttgcagggga cgacggatgt
     7141 gacgccgaaa cggtaacatc gtcggatgcg gcataaacgc cttatccgac ctggctgtaa
     7201 agcaaccgta ggccggataa gatgcgccag catcgcatcc agcgaccagg tcgcacggta
     7261 tcaacgaaat gacattaacg acgcatactc tttgacaaca tcatctgcca cttctgctcg
     7321 cggttaagtt ccgtgagcgg aacggcagga atacgcggcg tctttcttac gccgactaat
     7381 ttatcccggc tgttattgcg caggcgcgcg taaatttgct catcgatatc aaccactttt
     7441 accagtcgct ggcactggca tccccgccct tccagcttat tagggatcat cttaacttcg
     7501 cagcttattt ctgtgacttc cgacagaata taagtcaggg tattaaccgc cttgctgtgt
     7561 tcaatatcga aatgaataga gatctctctt gctatcaccc accggttttc agccatcatc
     7621 cattcggcga ttaacaaata gagtggtttt tcaacacatc cttcacacat acgaactcca
     7681 gtttcacaaa ttaaaagact ggctatgact cgttttgcag caattaaaca aggccatatt
     7741 aaaattgtgg atatatcgat agtatgcaac ttcaccgtcg ataagtgtga gctaaatcct
     7801 gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaacgg
     7861 tcctcatcag agaaccgtat tagttattcg atcaaattag cggatgaaaa atatccgcca
     7921 tgacacgcta ttatttgatc tgcgcgtgca tttcctgcac cgaaattact ttttcggtcg
     7981 catcggcatt cagcgccatc gcggtggcaa agccgccgtt cagggtggtg tcgtaatgca
     8041 ctttatattg cagcgcactg cgacggatca cgcgggagtc ttcaatcgca cgacgacctg
     8101 aggtggtgtt gatgatgtag gtatattcgc cattcttaat acggtcctga atgtgcggac
     8161 ggccttcatg caccttgttc accagacgcg gattgatacc cgcttcgccc agcacaatcg
     8221 ccgtgccgtg agtcgcatcc agctcgaagc cctgtttcag cagttttgcc gccaggtcca
     8281 ccacgcgttc tttatcgcct tcgcgcacgg aaagcagcgc acgaccgtgt ttcttcatgg
     8341 tggagttgct gcccagctgc gctttggcaa acgcttcagc gaaggtgcgg cccacgccca
     8401 ttacttcccc ggtagagcgc atttctggcc ctaacagcgg gtcaacgccc gggaatttat
     8461 tgaacggcag caccacttct ttcaccgagt agtacggcgg gataacttct ttggttacgc
     8521 cctgctcagc cagcgatttg ccagccatca cacgcgccgc cacttttgcc agcggtacgc
     8581 cggtggcttt ggagacgaac ggaacggtac gcgccgcacg cgggttaact tcaatcaggt
     8641 agacttcgtt gtttttcacc gcaaactgca cgttcatcag gccgcgcacc tgcagttcga
     8701 aggccagttt ctgcacctgc tggcgcatca catcctgaat ttcctgactt aaggtgtaag
     8761 ctggcagtga acatgcggag tcaccggagt gcacacccgc ctgctcaata tgctccatga
     8821 tgccgccaat cagcaccatt tcgccgtcgc agatggcatc cacgtcaact tctactgcgt
     8881 catcaaggaa gtggtccagc aacactggcg catcgttaga tacgctgacc gctgtctgga
     8941 agtagcgacg caggtcagct tcgtcataga cgatttccat cgcccgaccg ccgagaacgt
     9001 aagacggacg caccaccagc gggtagccaa tctctttcgc tttctcaacc gccatttcga
     9061 tggtggtaac ggtggcgttc gccggttgtt tcagtttcag acgctcaacc gcatgctgga
     9121 agcgttcacg gtcttccgca cggtcgatgg catccgggct ggtaccgata accggtacgc
     9181 cagcagcttc cagcgcgcgc gccagtttca gcggggtctg accgccgtac tggacgataa
     9241 cgcctttcgg cttctcaata cgcacaattt ccagcacgtc ttccagagtt accggctcga
     9301 agtagagacg gtcagaggtg tcgtagtcgg tggagacggt ttccgggtta cagttaacca
     9361 taatggtttc ataaccatct tcgcgcagcg ccagcgaggc gtgtacacag cagtagtcga
     9421 attcgatacc ctgaccgata cggttcgggc cgccgccgag caccatgatt ttttcacgat
     9481 cggtggacgg attcgcttcg cactcttctt cataagtgga gtacatgtaa gcggtgtcgg
     9541 tggcgaactc tgccgcacaa gtatccacgc gcttgtaaac cgggtgcaga tcatactggt
     9601 cgcgcaactt gcggatttcc gcttcgcgca cgcccgccag tttagccaga cgcgcatcgg
     9661 caaagccttt gcgtttcaac tggcgcagga attcagcgtg caggccagtg atgcccactt
     9721 ctgcgacttt ctcttccaga cgcaccagct cttcaatctg taccaggaac cagcggtcaa
     9781 tgttggtcaa attaaataca ccgtctacag acaggcccgc gcggaaggca tcagcgatat
     9841 accagatacg ctctgcgcca gcatctttca gttcgcggcg gattttagtc agcgcttccg
     9901 ggtcgtcgag actcactttc ggatcgaagc cagttgcgcc cacttccagg ccgcgcagcg
     9961 ctttttgtag ggattcctgc tgcgtgcgac caatcgccat cacttcgcca accgatttca
    10021 tctgagtggt cagacggtcg ttagcaccgg cgaatttctc gaagttgaag cgaggaattt
    10081 tagtaaccac atagtcgatg gacggctcga aggaggctgg agtacgccca ccggtgatgt
    10141 cgttcatcag ctcgtcgagg gtgtaaccca ccgccagttt cgccgccact ttggcaatcg
    10201 ggaaaccagt ggctttcgac gccagcgcag aggagcgaga cacgcgtggg ttcatttcga
    10261 taacaatcag gcgaccgttt ttcgggttca ccgcaaactg gacgttggaa ccaccggttt
    10321 caacgccgat ttcacgcagt accgccatcg aggcgttacg catgatttga tattctttgt
    10381 cggtcagcgt ttgggctggc gcgacagtga tggagtcacc ggtgtggatg cccatcgcat
    10441 caaagttttc gatagagcag acgatgatgc agttgtcgtt tttatcacgc accacttcca
    10501 tctcgtactc tttccagccg atcagcgact catcaatcag cagctcttta gtcggagaaa
    10561 gatccagacc gcgggcgcaa atttcttcaa attcttcacg gttataagcg ataccgccac
    10621 cgctaccgcc catggtaaag gatgggcgaa taatgcacgg gaagccaact tcagcggcaa
    10681 ccgccagcgc ttcttccatt gaatgagcga taccggaacg cgcagtttcc agaccgattt
    10741 tcttcatcgc tacgtcgaaa cggcggcggt cttcggcttt atcaatcgca tcggcagtgg
    10801 caccaatcat ggtgacgccg aactcttcca gcacgccctg acgctcaagc tccagcgcac
    10861 agttcagcgc cgtctgcccc cccatggttg gcagcaccgc gtccggacgc tctttttcaa
    10921 taatcttgcg taccacttcc cagtgaatcg gctcgatgta ggtggcatca gccatttccg
    10981 ggtcggtcat gatggtcgcc gggttggagt tcaccagaat gacgcggtaa ccctcttcac
    11041 gcagggcttt acacgcttgc gcgccagagt agtcaaactc acacgcctga ccgataacaa
    11101 tcgggcccgc acccagaatc aggatacttt ttatatctgt acgttttggc atggctcttt
    11161 tactcctgat tacttagcgg ttttacggta ctgctcaatt aacgcgataa agtggtcaaa
    11221 caacggcgcg gcgtcgtgtg gaccagggct ggcttccggg tgtccctgga agctgaacgc
    11281 cggtttatcg gtacgatgaa tgccctgtaa cgtaccgtcg aacagggatt tatgtgtgac
    11341 acgcaggttt gcaggtaatg ttgcttcatc caccgcaaaa ccgtggttct gggcggtgat
    11401 catcaccacg tttttctcaa catctttaac cggatggttg ccgccgtggt gaccaaattt
    11461 cattttgatg gtcttcgcac cgctcgccag cgccagcagc tgatgaccga gacagatgcc
    11521 gaataccgga atatcggttt cgaggaattt ctggatggcg gtaatggcgt aatcgcacgg
    11581 ggccgggtcg ccaggaccgt tggagagaaa gatgccgtct ggattcattt tcagcacatc
    11641 ttccgcagaa gtttgcgccg gaacgatggt caggcgacag cctctatcca ccagcatacg
    11701 caggatgttg cgcttggcac cgaaatcgta ggccacgacg tggtacggca gctcgtcttc
    11761 ttttttcgct tctggcaggc cgccggtcag tgtccagctc ccttgtgtcc agctataggg
    11821 ttctgcggtg gttacttctt ttgccagatc catgccgttc agacctggga acgcgcgggc
    11881 tttttctaac gccagcgccg catccgggtt gtcacccgca ataatgcagc cattctgagc
    11941 acctttctca cgcagtaaac gcgtcagctt acgggtatcg atatcggcaa tcgccacgat
    12001 gttatggcgc ttcaggtaag aagagaggtc ttcggtatta cggaagttgc tggcaatcag
    12061 cggcaggtcg cgaatcacca gaccttgggc atgaacctga gaagattctt catcggcgtc
    12121 attagtgccg acattgccaa tatggggata agtaagagta acgatttgac gagaatagga
    12181 aggatcagtg aggatttctt gataaccggt cattgaagta ttgaaaacga cttccccaac
    12241 cgccgaacct gttgccccta tggcccgacc gtgaaactgg gttccgtctt ccagaaccaa
    12301 tagcgctgac ttaatcaaaa caccctccag agaatattca ctcactttat ttgcatatta
    12361 attcacaatg atgacgtaaa tcaatgcaaa tctgcttacc gacgaatttc tggcaaacgg
    12421 cggcattctg gagatatggg agctaaaagt caacttaatg gcgggatttt ttatattatt
    12481 ttgtggctca tgcatgcttt acaggcatta agcgacaaaa agatcatata acatgttctg
    12541 gaaaacgctt gcgcagcaag aaga
//
LOCUS       DADSZD010000130        11881 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.131, whole genome shotgun sequence.
ACCESSION   DADSZD010000130 DADSZD010000000
VERSION     DADSZD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.131"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..3640)
                     /locus_tag="J1Z28_003330"
     CDS             complement(<1..3640)
                     /locus_tag="J1Z28_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBA7718139.1"
                     /translation="MRIVIDLQGAQTESRFRGIGRYSIAIARGIIRNNSRHEIFIALS
                     AMLDESIADIKAQFADLLPAENIVVWHAVGPVRAMDQGNEWRRESAELIREAFLESLC
                     PDVVFITSLFEGHVDDAATSVHKFSRQYKVAVLHHDLIPLVQAETYLQDDVYKPYYLQ
                     KVEWLKNADLLLTNSAYTAQEAIEHLHLQGDHVQNIAAAVDSQFCMAEVAASEKETVL
                     GHYGIQREFMLYAPGGFDSRKNFKRLIEAYAGLSDALRRSHQLVIVSKLSIGDRQYLE
                     SLASGNGLQQGELVLTGYVPEDELIQLYRLCKLFIFASLHEGFGLPVLEAMSCGAPVI
                     GSNVTSIPEVIDNPEALFDPYSVSSMRDKIAQCLTDDTFLARLKEMAQQQARNFSWDK
                     AAVTALEAFEKIAVEDTGTAQVLPEALIQKILAIPQGQPDDRDLRLCATAIDYNLKTA
                     ELYQIDDKSLNWRVEGPFDSSYSLALVNREFARALSADGVEVLLHSTEGPGDFAPDAS
                     FMAQSENSDLLAFYNQCQTRKSNEKIDILSRNIYPPRVTKMDAKVKFLHCYAWEETGF
                     PQPWINEFNRELDGVLCTSEHVRKILIDNGLNVPAFVVGNGCDHWLNIPAETTKDVDH
                     GTFRFLHVSSCFPRKGIQAMLQAWGKAFTRRDNVILIIKTFNNPHNEIDAWLAQAQAQ
                     FIDYPKVEVIKEDMSATELKGLYESCDVLVAPGCAEGFGLPIAEAMLSGLPAIVTNWS
                     GQLDFVNSQNSWLVDYQFTRVKTHFGLFSSAWASVDIDNLTDALKAAASTDKSVLRDM
                     ANAGRELLLQQFTWKAVADRSCQAVKTLRAHIDIAQHRARIGWLTTWNTKCGIATYSQ
                     HLVESAPHGTDVVFAPQVSAGDLVCADEEFVLRNWIVGKESNYLENLQPHIDALRLDV
                     IVIQFNYGFFNHRELSAFIRRQHDAGRSVVMTMHSTVDPLEKEPSWNFRLAEMKEALA
                     LCDRLLVHSIADMNRLKDLGLTANVALFPHGVINYSAASVTRQQQSLPLIASYGFCLP
                     HKGLMELVESVHRLKQAGKPVRLRLVNAEYPVGESRDLVAELKAAAQRLGVTDLIEMH
                     NDFLPDAESLRLLSEADLLIFAYQNTGESASGAVRYGMATQKPVAVTPLAIFDDLDDA
                     VFKFDGCSVDDISQGIDRILNSIREQNSWATRTQQRADAWREQHDYQAVSRRLVNMCQ
                     GLAKAKYFK"
     gene            complement(3643..4920)
                     /locus_tag="J1Z28_003331"
     CDS             complement(3643..4920)
                     /locus_tag="J1Z28_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBA7718140.1"
                     /translation="MGSSFYRSFEERHRGSVEEIKRRLSFYLPFLAGLKDIYPDGMIA
                     DIGCGRGEWLEILTENGIANIGVDLDDGMLARAREAGLNVQKMDCLQFLQSQADQSLI
                     ALTGFHIAEHLPFEVLQQLVMHTLRVLKPGGLLILETPNPENVSVGTCSFYMDPTHNH
                     PLPPPLLEFLPIHYGFNRAITVRLQEKEVLQSPDAAVNLVDVLKGVSPDYSIIAQKAA
                     PTDILERFDTLFTQQYGLTLDALSNRYDAILRQQFSSVVSRLETLNQTYMQQISQMSE
                     TIQTLQGEVDDLSHVIDQNHQLHQQMADLHNSRSWRITQPLRWLSLQRQLLRQEGAKV
                     RARRAAKKILRKGMALSLVFFHRYPKSKVYLFKVLRKTGCYTLLQRLFQRVMLVQSDT
                     MMMQSRRYDVGTEEMTSRAMSIYNELKNKNTEK"
     gene            complement(4920..6134)
                     /locus_tag="J1Z28_003332"
     CDS             complement(4920..6134)
                     /locus_tag="J1Z28_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003656103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen ABC transporter ATP-binding
                     protein Wzt"
                     /protein_id="HBA7718141.1"
                     /translation="MSYIRVNNVGKAYRQYHSKTGRLIEWLSPLNTKRHNLKWILSDI
                     NFEVAPGEAVGIIGINGAGKSTLLKLITGTSRPTTGEIEISGRVAALLELGMGFHSDF
                     TGRQNVYMSGQLLGLSSEKITELMPQIEEFAEIGDYIDQPVRVYSSGMQVRLAFSVAT
                     AIRPDVLIIDEALSVGDAYFQHKSFERIRKFRQEGTTLLLVSHDKQAIQSICDRAILL
                     NKGQIEMEGEPEAVMDYYNALLADKQNQSIKQVEHNGKTQTVSGTGEVTIAEVHLLDE
                     QGNVTEFVSVGHRVSLQVNVEVKDDIPELVVGYMIKDRLGQPIFGTNTYHLNQTLTSL
                     KKGEKRSFLFSFDARLGVGSYSVAVALHTSSTHLGKNYEWRDLAVVFNVVNTEQQEFV
                     GVSWLPPELEIS"
     gene            complement(6134..6928)
                     /locus_tag="J1Z28_003333"
     CDS             complement(6134..6928)
                     /locus_tag="J1Z28_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen ABC transporter permease
                     subunit Wzm"
                     /protein_id="HBA7718142.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFRKHAADMVDEL"
     gene            complement(6930..8306)
                     /gene="cpsG"
                     /locus_tag="J1Z28_003334"
     CDS             complement(6930..8306)
                     /gene="cpsG"
                     /locus_tag="J1Z28_003334"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HBA7718143.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPEKVVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRANAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLSKISVLKEYVDHLM
                     SYVDFANFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVTELLCLKNSSLKSLVADRQKAFPASGEINRKPGNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     gene            complement(8330..9745)
                     /locus_tag="J1Z28_003335"
     CDS             complement(8330..9745)
                     /locus_tag="J1Z28_003335"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HBA7718144.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGRNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHSAYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLIELAKYRPDIL
                     ETCQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDAEWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRAEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGAYLGEDDIIRIKDQYGRC"
     gene            complement(10019..10302)
                     /locus_tag="J1Z28_003336"
                     /pseudo
     CDS             complement(10019..10302)
                     /locus_tag="J1Z28_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10571..10696
                     /locus_tag="J1Z28_003337"
                     /pseudo
     CDS             10571..10696
                     /locus_tag="J1Z28_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="catalyzes the formation of 1-(5-phosphoribosyl)-AMP
                     from 1-(5-phosphoribosyl)-ATP and the subsequent formation
                     of
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide from 1-(5-phosphoribosyl)-AMP in histidine
                     biosynthesis; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase"
     gene            complement(10897..11874)
                     /gene="wzzB"
                     /locus_tag="J1Z28_003338"
     CDS             complement(10897..11874)
                     /gene="wzzB"
                     /locus_tag="J1Z28_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBA7718145.1"
                     /translation="MRVENNNVSGQNLDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVNQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLEQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTLEVVAQEQKELRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSDALESMVKHEASRPLVFSSNYYQTRQNLLDIDNLDVDELDIHA
                     YRYVMKPTLPIRRDSPKRAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT         3105 a         3169 c         2716 g         2891 t
ORIGIN      
        1 atttaaaata tttagcttta gctaagcctt gacacatatt aaccagacgg cgtgaaacag
       61 cttgataatc atgttgttcc cgccatgcat cggcacgttg ttgagtcctg gttgcccaag
      121 agttctgttc acggatggaa ttcaggatcc ggtcaatccc ctgactgata tcatcgacgc
      181 tgcatccatc aaatttaaag acggcatcgt ccaaatcatc aaagatcgcc aggggcgtta
      241 ccgcaacagg tttttgagtc gccataccat aacgtaccgc cccgctagca gactccccag
      301 tattctgata agcaaaaatc agaagatcgg cttctgaaag caaccgcaga ctctctgcat
      361 cagggaggaa atcattgtgc atctcaatca gatcggtaac acctaaccgc tgagcagccg
      421 ctttaagctc tgccaccaga tcgcgtgact ccccaacagg atactctgcg ttcaccagtc
      481 gtaaacgaac cggtttacca gcttgcttga gtctatggac ggattctact agttccatca
      541 ggcccttatg cggtaagcag aagccatagc tcgcaattag cggtaaagac tgctgttgac
      601 gcgtgacgct cgctgcggag tagttgataa cgccgtgcgg gaataaagca acattcgcag
      661 ttaagcctaa atctttaagg cggttcatat cggcaatcga atgcaccaac aaccggtcgc
      721 aaagtgccag cgcctctttc atttcagcaa gacggaaatt ccagctcggc tctttttcca
      781 gcggatccac agttgagtgc atcgtcataa caactgaacg accggcgtca tgctggcgac
      841 gaataaacgc cgacagttct cgatgattaa agaatccata gttgaattgg atcacgatga
      901 catcgagtct cagagcatca atgtgtggct ggaggttttc cagatagttg ctctctttac
      961 ctacaatcca gttgcgaagt acaaactctt cgtctgcaca cacaagatcg ccagcgctga
     1021 cctggggagc aaaaacaaca tccgtgccat gaggtgcgct ctccaccaga tgctgggaat
     1081 aggttgcgat cccacatttc gtgttccagg tcgtcaacca gccaatgcgc gcccgatgct
     1141 gtgcaatatc aatatgcgca cgcagagtct tgaccgcctg gcaagaacga tcagccaccg
     1201 ctttccaggt aaactgctgc agaagaagct cgcgaccagc attggccatg tcacgcagca
     1261 ctgatttatc ggttgaggct gccgctttta atgcatctgt taagttgtca atatccacac
     1321 tggcccaggc tgaggaaaac agaccaaagt gcgtttttac ccgagtgaac tgatagtcaa
     1381 ccagccatga attttgtgaa ttaacaaaat caagttgccc gctccaattg gtgacgatag
     1441 ccggtagccc actcagcatt gcttcagcaa taggtaaacc aaagccttca gcgcaacctg
     1501 gagcaaccaa aacatcacag ctttcataaa gccctttaag ctcggtggct gacatatcct
     1561 ctttgatcac ttcaactttg ggatagtcta tgaattgagc ctgggcctga gccagccatg
     1621 cgtcaatttc attgtgcgga ttgttaaaag tcttaatgat taagataaca ttgtcacgac
     1681 gagtgaacgc cttcccccaa gcctgaagca ttgcctgtat ccctttgcgt gggaaacaag
     1741 aagagacatg caggaaacgg aatgttccgt gatccacatc ttttgtcgtc tcggctggga
     1801 tattgagcca atggtcacag ccattgccaa caacaaatgc gggcacattc agtccgttat
     1861 caatcaatat cttacgaaca tgttccgaag tacacagcac tccgtcaagt tcccgattaa
     1921 attcattgat ccacggttgc ggaaagcccg tttcttccca agcataacaa tgaaggaatt
     1981 ttactttggc atccattttg gtaacccgcg gtggatagat atttctgcta agaatatcta
     2041 tcttttcgtt actcttgcgg gtctgacatt gattataaaa tgccagaaga tcactatttt
     2101 ccgactgtgc cataaacgag gcatctgggg caaaatcacc tggtccttca gtggaatgca
     2161 ataaaacctc tacaccatcg gctgagagtg cccgggcaaa ttcgcggttg accaacgcca
     2221 gactatatga gctatcgaat gggccttcca cacgccagtt cagcgattta tcgtcgattt
     2281 gataaagttc tgccgttttc agattgtaat caatggccgt tgcgcacaag cgcagatcgc
     2341 ggtcatctgg ctgcccttgt gggatagcaa ggatcttctg aatcaaagct tcaggcaaaa
     2401 cctgcgcagt accggtgtct tctaccgcga tcttttcgaa agcttccaga gcagtcaccg
     2461 cagccttatc ccaagagaaa ttacgcgctt gctgctgcgc catttctttc agacgcgcga
     2521 ggaaggtatc atcagtcaaa cattgcgcga tcttatccct catggaagag acagaatacg
     2581 ggtcgaataa tgcctcagga ttatcgatga cttcaggaat actggtgaca tttgagccaa
     2641 tcaccggcgc accgcacgac attgcttcca gaaccggcaa cccaaaacct tcatgtagtg
     2701 aagcaaagat gaacagctta cataggcgat agagctggat cagctcatct tcaggcacat
     2761 aaccagtgag taccagttcg ccctgctgta aaccattacc tgacgcaagg gattccagat
     2821 actgacgatc accgatggaa agcttactga cgataaccag ttgatgactg cgacgtaagg
     2881 catcactgag cccggcatag gcctcaatca accgtttaaa gtttttcctt gagtcaaatc
     2941 ctccgggcgc atacaacatg aactcgcgct gaataccgta atggccaagg acggtctctt
     3001 tttcgctcgc tgccacctcc gccatacaaa actgagaatc gactgcggct gcaatattct
     3061 gcacatgatc gccctgtaaa tgcagatgct caatcgcttc ttgtgcggta taagcagagt
     3121 tagtcaacaa aaggtcagcg ttttttaacc actcaacttt ctgtaagtag tagggtttgt
     3181 atacatcgtc ctgcagatag gtttccgcct gcacgagggg gataagatcg tggtgcagta
     3241 cggctacttt atactgacga ctaaatttgt gtaccgatgt agccgcatcg tcgacatgac
     3301 cttcaaacaa actcgtaatg aaaacgacat ctggacacaa tgattcaaga aacgcttccc
     3361 gaatcagctc tgcgctctcc cgacgccatt cattaccttg gtccatcgca cgaacagggc
     3421 ctacggcatg ccatacgact atattttctg ccggcaggag atcggcaaat tgcgccttaa
     3481 tatctgcaat cgactcatcc agcatggcgg atagcgcgat gaaaatctca tgccggctgt
     3541 tatttctgat tatgcctctg gcgattgcga tactataacg accgatgcca cgaaagcggc
     3601 tttccgtctg tgcgccttgt aaatctatga caatacgcat cgttatttct ccgtattttt
     3661 attttttaat tcgttataaa tactcatcgc gcgacttgtc atttcttcag tacccacatc
     3721 atatcttctg gactgcatca tcatcgtgtc agattgcacc agcattacgc gttggaacaa
     3781 acgttgtagc aatgtataac agccagtttt tctcagaacc ttaaacagat aaaccttaga
     3841 cttagggtaa cgatggaaaa agaccagcga gagcgccatc cctttgcgca atattttttt
     3901 cgcagcccta cgggctcgca ctttagcccc ttcctgacgt aataattgac gttgcaaaga
     3961 caaccagcgt agtggttgag taatacgcca tgaacgactg ttatgtaaat ccgccatttg
     4021 ctgatgaagc tgatggttct gatcgatgac atgactcaga tcgtcaacct caccttgcaa
     4081 cgtctgaata gtctctgaca tttggcttat ttgttgcata taggtttggt tcaacgtctc
     4141 cagccgtgag acaacggacg aaaactgttg gcgcaaaatc gcatcgtaac ggttgctcag
     4201 agcatccagc gtcagaccgt actgctgggt aaacagggtg tcaaagcgtt caagaatatc
     4261 tgttggcgct gctttctgag caatgatgct gtagtcgggg ctcacccctt tgagtacatc
     4321 gaccaaatta acggctgcat ccggagattg aagaacctct ttttcctgca gacgaacggt
     4381 aattgctcgg ttaaaaccat aatgaatagg taaaaactca agcagtggcg gtggcagagg
     4441 atgattatgc gttggatcca tataaaatga acaggtgccg acgcttacat tctccgggtt
     4501 aggcgtttcg aggatcagca aaccacctgg tttcagcacc cgtagggtat gcatgacgag
     4561 ctgctgcagg acctcaaaag gcaaatgctc agcaatatga aaaccggtca acgctatcag
     4621 gctctgatcc gcctgacttt gcagaaactg cagacaatcc attttctgca cattcagtcc
     4681 ggcctccctg gcgcgcgcca gcatgccatc atcgagatcg acgccgatgt ttgcaatgcc
     4741 attttcagtc aggatctcca accattcgcc acgtccacaa ccaatatccg caatcatgcc
     4801 atcaggataa atgtccttca gacctgcaag aaaaggtaaa taaaaactca agcggcgctt
     4861 gatttcttca accgaacctc tgtgtcgttc ttcaaatgaa cgataaaacg acgaacccat
     4921 taagaaatct ccagttcagg cggcaaccag gacacgccga caaactcttg ttgttccgtg
     4981 ttaacgacgt tgaataccac ggccagatcg cgccattcat agtttttgcc gaggtgcgta
     5041 ctggaagtat gcaacgcgac agcgacagaa taggagccaa cccccaatct cgcatcgaaa
     5101 gaaaataaga acgaacgctt ttctcctttt ttcagggagg tgagtgtctg attgagatgg
     5161 tacgtattgg tcccgaaaat cggctgccca agtcgatcct taatcatata tccgacaaca
     5221 agctcaggaa tatcgtcctt gacctcaacg ttgacctgca agctgacacg atgccctacc
     5281 gaaacaaatt cagtcacatt gccctgttca tcgagaagat gaacctcagc gatagtcacc
     5341 tcaccagtgc ctgaaacagt ttgcgtttta ccattatgct caacttgttt aatggactga
     5401 ttttgtttat cggccagaag cgcattgtaa taatccatca ctgcttcagg ttcaccttcc
     5461 atttcaattt ggcctttatt caataaaatg gcccggtcgc aaatgctttg gatcgcttgt
     5521 ttatcatggg ataccagcaa cagcgtggtg ccttcctgac gaaattttcg aatacgctca
     5581 aagcttttat gctggaaata tgcatcccca acagataatg cctcatcgat aattagcaca
     5641 tcaggacgga tagccgtcgc tacactaaaa gctaatcgaa cttgcatccc actggagtag
     5701 acgcgcacag gctgatcgat ataatcccca atctcagcaa actcttcaat ttgcggcatc
     5761 agttcagtta ttttctctga cgataacccc aacagttgcc cagacatata aacattctgc
     5821 cgaccagtga aatcagaatg aaaccccatc cccaattcga gtaatgcagc gacacgtcca
     5881 gagatttcaa tttctccagt cgtcggcctg gacgtcccgg ttatgagttt aagcagggta
     5941 ctcttgcctg caccgttgat accgataata ccgacagcct cgcccggagc gacttcgaaa
     6001 ttaatgtcac tgaggatcca tttcaaatta tggcgtttgg tattcagagg ggataaccat
     6061 tcgatcagtc tcccggtctt tgagtgatac tggcgatacg ccttaccgac attatttact
     6121 ctgatataac tcattataat tcatccacca tatccgccgc atgcttgcgg aacatcctga
     6181 aaccgatgac gcagaaaata atggcgagag ccaatactgg ccatagcgaa taccagttgg
     6241 gggccagatg ataggcgaag atggactgat aagattgcat gatccgagtc atcgggttat
     6301 acatcatcag attttttgcc catgcaggta atgaattcag tacataaaca atgggtgtga
     6361 accaaaacca gaattgcagc gcaacgccaa ccagttgccc cacatccctg aaaaagacgt
     6421 tcatcacacc aaggatcatc cccagcccac cggcaaacag gatctgcaaa agcaggaccg
     6481 gtatcaccga gagaaagagc cagccgggga agttaccggt gacaatgata aaaattagaa
     6541 acaaactgaa aataatcgcg aaatttagca ccgccgataa cgtcacgatg atcggcagac
     6601 agattttcgg aaaactgagt ttcttgatca gattagcatt gttaataaat acgctctgac
     6661 ctttatccag catctcagta aataatcccc aggtcagtac cccggaacag agataaatac
     6721 tataggcaaa cgacccggta ttatcgggca ttcttgcctt catcacctcg gaaaaaacca
     6781 gggtatagac cagaatcata gagagcggtt gtaaaacgag ccatagtgcg cccagcatac
     6841 tagtttgata gcgcgcctga aaatcacgtt taacactgct ccagataaat ccccgataac
     6901 gataaatcgt cgttagtaaa tctctcacct tattccccgc ttaacaggtg taacagctcg
     6961 gtcgtttttt cattcataag cgccacatca gctctggact caacgttcag acgcaccacc
     7021 ggctcggtgt tagacgtgcg caggttaaag cgccattcag ggtattcaat actgatcccg
     7081 tccgttgtgt cgatgtgtgc agccgccggc tcatactgcg cccggatgcg ggcgatcgcc
     7141 tcagccgcat tccctggctt gcggttaatc tctcccgacg cagggaacgc cttctggcgg
     7201 tccgccacca gcgatttcag cgagctgttc ttcagacaca gcagctccgt caccagcagc
     7261 cacgggatca tcccgctgtc gcagtaggcg aaatcgcgga agtaatggtg cgcactcatc
     7321 tccccgccgt agatagcgtc ttcctgacgc atccgctcct tgatgaacgc atgccccgtc
     7381 ttcgacatca ccggctggcc gccgttgcgg gtcacgatgt ctaccgtgtt ccacgtcaag
     7441 cgcgggtcgt gaatgatttt cgctcccggc tgcttctgca ggaacgcctc agccagcagg
     7501 ccgacaatgt aatacccctc gataaacgaa gcttcgtcat cgaacaggaa gcagcgatcg
     7561 aagtcgccgt caaaggcaat ccccatgtcg gcctgatgct cgcgcaccgc gtcggcggta
     7621 tcctggcggc actccggcag cagcggattc ggaataccgt tagggaaatg gccatccggc
     7681 tggtgatgca ccttgataaa ggttaccggc accccagccg ccgcgaagcg tttctccacc
     7741 tcatcaatca cgtgccccgc agccccgttt ccggagttca ccaccaactt cagtggacgg
     7801 gtgaagttcg cgaagtccac gtagctcatc agatggtcaa catactcctt cagtaccgat
     7861 atcttgctca gggtcccgcg acgcgccggg tccactggcg ggaactggtt ttcctccgcc
     7921 aggcgctgga tatcccgcag gccggtgtcg ccgctgatgg gcttcgcatt cgcgcgcacc
     7981 agcttcatgc cgttgtagtt cataggattg tggctcgctg tcacctcgat gccaccatct
     8041 actccaaggt ggaaggtggc aaagtaaatc tcttcggtac cgctcaggcc gatgtccagc
     8101 acgtcagtac cggcatccat tagcccgcgg gccagcgcca gcttcagcga ctcgcttgtg
     8161 aggcgcacat cgccccccac cactaccttc tcgggtttaa gaaattcgcc gtaggcgcga
     8221 ccgatacggt aggcaatgtc ctcgttcagt tcctcaccca gttcaccacg gatgtcataa
     8281 gccttaaaac aagttaactg tgtcattctg cgtcttgtcc cgaaaataat tagcaacgac
     8341 catactggtc tttaatacga ataatgtcgt cctcgccaag gtacgccccc gactggatct
     8401 ccagcacttc cagcggaatg cagccagggt tttccaggca gtgctcggcg ccaatcggaa
     8461 taaaggtgga ctggttctcg gacagcagga actgcttacc gttgaccgtc acctgaccgg
     8521 tgccggcgag aataacccaa tgctcggcgc gatggtggtg catctgcatc gagaaggcac
     8581 cgcctggttt caccgtgata cggttgacgt tgaagcgcgg ggtctggacc accacgtcgc
     8641 agcggcccca ggggcggtaa atctcacggt ggcgcttgta ctcgctgcgc tgattctgct
     8701 tgaggaactc gaccgctttc ttcacgtcct gggaacgctc gcgattcatc accagcacgg
     8761 cgtccttagt gctgacaatc accagattct ctacgccaat ggccgccact agcttctcgt
     8821 cgctgttgat gtagcagttt tcgctgttgt gtacccacgc atcgccacta aggacgttgc
     8881 cctgctcgtc tttcgggctg acctcccata gtgcggacca ggagccaacg tcgctccact
     8941 cagcatcgag accgaccacc accgcatcag cggttttctc cataacggcg tagtccacgg
     9001 actcatccgg gcactcgcag aaaatatcat gtgggatatt gatgaaatcg ctgccattat
     9061 cggcggcatt caccgcagcc tggcaggttt ccaggatatc cggacggtat ttggccagct
     9121 cgatgagata tttcttggcg cggaacataa acatgccgct gttccagtag tactccccgg
     9181 aggcgaggta agcctcggcg cgctcgcggt ccggcttctc cacaaagcgg gccacctggt
     9241 acgcggaatg ggcactgtcg gtgagcgcca cgccgcgctg aatgtaaccg tagccagttt
     9301 ccggggcatt cggcacgata ccgaatgtga ccagatgacc ttcatcagca tactgctcgg
     9361 cgacccggat agcatcgtgg aaggccgact ggttattgat gatgtggtcg gcggcgagaa
     9421 ccagcatcag cgggtcgtca ccgtagcggg tggcctgaag ggccgccagg gcgatggccg
     9481 gcgcggtgtt gcgcccgacc ggctcaagta taatgttatt cgacagctta ttgagctggc
     9541 gcagttgttc agccaccagg aagcgatgct cttcgttaca gatgaccatc ggttcatgga
     9601 tttccagacc cgagagtcgg gtgatggttt cctgcagcat ggagttccgc ccgaacaggc
     9661 ggaggaactg tttcggataa agctcgcgtg acatcggcca gagacgactg ccggtaccac
     9721 cagccataat tacaggaagt aacataatga aatccttaaa taaataattg ttttatttta
     9781 ctatcaatca atcgaccaac gggttgaatg gcctttttca ggtacgctac cgccattggc
     9841 tatcgcaacc aaaatgcgta gtgctctagg ctaaacgcca ccctctttcg cttcagactg
     9901 tcggcagccg cttcactcag cggtctcaaa aaccatcctt acggaacggg catcctgaaa
     9961 acctaaaagc agagatatgt cccagggcat atttctgctt ttaatatgta tctgttcctc
    10021 accaggccac gaaacgcccc acaatttcca catttgccgc cagagtaacc atcaggaacc
    10081 tctcctttac gcgggggaaa acacatgctc tcaccgccat caaaaaaacg aatcaagtga
    10141 tttaccgatg ccagccccca tcagaaaagg gcatttacga tacggtgaaa gataagaaga
    10201 gacatcgtat tcgccgccag agaagcagcg cgcattcggg cttgacagct ctttgcacac
    10261 gacgacggcg ttatgttgcg actggtggga taaacgactc atgcctctac actgcacatt
    10321 ccacaaaaaa atcttcgcag attatagcgt tgcatgctgg gaaaaaaaat ataaaagccg
    10381 actaattaat ggattagtca aataatacac acatcaaaca tttaccaaac caattaatgc
    10441 taattcattg atataacggt aaaaataaag aaaggcatga caattttttg cttatttgct
    10501 aatgatccgc tggtgtaaaa tatatctttg aataaaatta cgccatacta gaggtgaata
    10561 gccccatttt gcgacgatac ataactgctt cgaactgacc aatgaggcgt ctgatttcat
    10621 atatcacctg ctgaggcaga atcaagacca ggatttaaca acggtgattg agaacctgcg
    10681 caggtggcat cagtgaattg ctgggtatgt ggaagtgtta ttacagtcac ttccggcaac
    10741 aactaatccg ttaatgctcg gtgcgcaagt ttattgcgct tacaaaattc tcaacacatg
    10801 taagctggat aaggtgcgcc agcatcgcat ccaacaaaaa aaacgggcac gcgaccaccc
    10861 tgcaccaccc tgcccgcttt ctttaagcta aaaaaatcac ttcgcgttgt aattacgcag
    10921 cgcattacgc cccagcacaa tacccgcccc aaccatgcca cccagcagaa ccgccagaat
    10981 caaggtgatt gcccttttag gactatcgcg gcggatgggt aacgtaggtt tcatcacata
    11041 gcggtaagcg tgaatatcaa gctcatcaac gtcaagattg tcgatatcta gcaggttttg
    11101 acgagtctga tagtagtttg acgagaacac caacggacgg ctcgcttcat gcttaaccat
    11161 cgactccagt gcatcactcc ccaaaaggaa catggtatcc tgggtcacgt cctgtgtctg
    11221 ctgaatctgc ggctttgtca cctgtgcctg attcgcatac tgcaacgctt cctgaatttg
    11281 acggatacgc agctctttct gctcctgggc gaccacttcc agggtcttca gagaatcttg
    11341 caagtttttc atacgcagcg cgatgttgtc tttcaggtct ttttccagtt cttcatttac
    11401 ctggtcgtca acttgctgga tatactgctc cagctgtttc tgcgcacctt caggtgtttc
    11461 accaacataa gaaaccgcta aaggtaagga ttgattttta acggtaggtt caatggtcag
    11521 tttttcaggt tcttcctgat tatccagcgt ttctgctaat gcagaaaatg tcgtgctgaa
    11581 gcgaccaatt aacatcgact ggatctcaga cactttcggc gcagctgaac cataaatcac
    11641 atttatggcg ttgttataac cagcgatttg ccccacatca ggttgattaa cgatagctgt
    11701 ggaagtccat ttttctttgg caacgaccag atatcctatt gccagggcaa tagcgacaat
    11761 gacagagatg atgattgtca tcttgccacg ccatagttgc accagtaaat caatcaaatc
    11821 aatctgttcc gggtcaaggt tttgcccaga aacattatta ttttctactc tcatcattac
    11881 c
//
LOCUS       DADSZD010000131        11806 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.132, whole genome shotgun sequence.
ACCESSION   DADSZD010000131 DADSZD010000000
VERSION     DADSZD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.132"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            352..1116
                     /locus_tag="J1Z28_003339"
     CDS             352..1116
                     /locus_tag="J1Z28_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBA7718146.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1230..1928)
                     /gene="thiQ"
                     /locus_tag="J1Z28_003340"
     CDS             complement(1230..1928)
                     /gene="thiQ"
                     /locus_tag="J1Z28_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBA7718147.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGMTDELL
                     SGKASASAILGITG"
     gene            complement(1912..3522)
                     /gene="thiP"
                     /locus_tag="J1Z28_003341"
     CDS             complement(1912..3522)
                     /gene="thiP"
                     /locus_tag="J1Z28_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBA7718148.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     SVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGCHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVLVDGINRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            complement(3498..4481)
                     /gene="thiB"
                     /locus_tag="J1Z28_003342"
     CDS             complement(3498..4481)
                     /gene="thiB"
                     /locus_tag="J1Z28_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBA7718149.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPYDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFIEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(4512..4611)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4645..6300)
                     /locus_tag="J1Z28_003343"
     CDS             complement(4645..6300)
                     /locus_tag="J1Z28_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SgrR"
                     /protein_id="HBA7718150.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAA
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQILPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     SSNQLKIQAFDDFFGYRALIDEVNVWVLPEIADDPAGGLMLKGPQDEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWISYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHTRT
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEINLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            6389..6520
                     /gene="sgrT"
                     /locus_tag="J1Z28_003344"
     CDS             6389..6520
                     /gene="sgrT"
                     /locus_tag="J1Z28_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBA7718151.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(6628..7233)
                     /gene="leuD"
                     /locus_tag="J1Z28_003345"
     CDS             complement(6628..7233)
                     /gene="leuD"
                     /locus_tag="J1Z28_003345"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBA7718152.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVQANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(7244..8644)
                     /gene="leuC"
                     /locus_tag="J1Z28_003346"
     CDS             complement(7244..8644)
                     /gene="leuC"
                     /locus_tag="J1Z28_003346"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBA7718153.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(8647..9738)
                     /gene="leuB"
                     /locus_tag="J1Z28_003347"
     CDS             complement(8647..9738)
                     /gene="leuB"
                     /locus_tag="J1Z28_003347"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBA7718154.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTNEMGDIIARYVVEGV"
     gene            complement(9738..11309)
                     /gene="leuA"
                     /locus_tag="J1Z28_003348"
     CDS             complement(9738..11309)
                     /gene="leuA"
                     /locus_tag="J1Z28_003348"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBA7718155.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(11401..11487)
                     /gene="leuL"
                     /locus_tag="J1Z28_003349"
     CDS             complement(11401..11487)
                     /gene="leuL"
                     /locus_tag="J1Z28_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBA7718156.1"
                     /translation="MTHIVRFIGLLLLNASFLRGRRVSGIQH"
BASE COUNT         2640 a         3151 c         3167 g         2848 t
ORIGIN      
        1 catggcgaaa tatagataac atttttcttc agaaagcaaa gctaatagat tttttgaaaa
       61 gaatgggacc gagtctacaa aacgattgta tatggttttt tcatgataaa agtgattatg
      121 caaataattt ccagcgttat tgcatcattg agcaccgtga ttctctgcag gtggaatatt
      181 ttgagactat cgaatgatag gcagagggtg tcttttgtgc cggttgtgaa gaaaaagtga
      241 atgatgtagc cgtcaagttg tcataattgg taacgaatca gataattgac ggcttgacgg
      301 agtagcatag ggtttgcaga atccctgctt cgtccatttg acaggcacat tatgcaagca
      361 ttgctggaac actttattac ccaatccacc gtgtattcat tgatggcggt ggtgttggtg
      421 gcctttctgg agtcgctggc gctggtcggt ttgattctac ccggtacggt gctgatggcg
      481 gggctgggag cgctgattgg tagcggtgag ttaagtttct ggtacgcctg gctggcgggg
      541 attattggct gcctgatggg cgactggatc tccttctggc tgggctggcg ttttaaaaag
      601 ccgttgcatc gctggtcatt tctgaagaag aacaaagcat tactcgataa aaccgaacat
      661 gcgctgcatc agcacagcat gttcaccatt ctggtcggtc gttttgtcgg tccgacgcgt
      721 ccgctggtgc caatggtggc gggaatgctg gatctcccgg tggcaaaatt tattacgccg
      781 aatattatcg gctgcttgct gtggccaccg ttttacttcc tgccggggat tctggcgggt
      841 gcggcgatcg atattcctgc cgggatgcag agcggtgagt ttaaatggtt gctgctggca
      901 acagcggtgt ttttgtgggt tggtggctgg ctgtgctggc ggttatggcg cagcggtaaa
      961 gcgactgacc gtttgagtca ttatttgtcc cgcggtcgtt tgttgtggct gacgccgttg
     1021 atttctgcca tcggcgtggt ggcgctggtg gtgttaattc gccacccatt gatgccggtg
     1081 tatatcgata ttttgcgtaa agtggttggg gtttagggga tagccttgtg cgggttgcct
     1141 gatgcgacgc ttgccgcgtc ttatcaggcc tacaaaacgc actgtccgta ggtcggataa
     1201 ggcgttcacg ccgcatccga cagtgcatac taacccgtaa tccccaatat tgccgaagca
     1261 ctcgccttac cgctcaacaa ctcatcggtc ataccctgcc aggcgatgcg cccgtcggcg
     1321 accaccaccg agcgtgtggc gatccgcgcc gcatcttcca cactgtgcga caccatcaat
     1381 agcgtcattt tttgctgctg gcagctcgtg ctcaccagcg tcaacatctc ttgacgtaac
     1441 gccggatcga gcgcagagaa cggttcatcg agcaataaaa tcggctgttc gcgcaccaga
     1501 caacgcgcta acgccacgcg ctgtcgttga ccgccagaaa gctcgcccgg taatcgcgcc
     1561 attaaattat caatccccat ctgacgggca atcgcgtgca ttttctcctg ctgtgccgcg
     1621 ttcagcttca atcctggatt aagccccagc ccgatgttct gcgcgaccgt caggtgactg
     1681 aacaggttgt tttcctgaaa cagcatcgac accggacggc gtgacggcgg cgtcgtggtg
     1741 tgatcaacgc cgtcgatagt cagcgaaccg ctggctggcg tcagaaaacc ggcgatcaaa
     1801 ttcagcaggg tacttttacc cgcgccgctt ggcccgagga tcgccacctg ctcgccgcgt
     1861 tccaccgtta agctaaaacg catcggcaaa tggtggtaaa gccaggtgat atcagtcagt
     1921 tttaacattt cgccccggta gtttttcaat cacggtaaac agcagaaaac agagtagcag
     1981 cagaattaac gcggtgaccg cgccgtcctg gctgcgatag gagccaattt gctggtagag
     2041 ataaaacggc agggtgcgga aatcatcgtt accgaacaac gccaccacgc caaaatcacc
     2101 aatcgatagc acacaagcaa aggccagtgc ctgcgccagt gggcgtttaa gggcgcgcag
     2161 ctccaccact tttaagcgtg accagccttc aatgcccagc gactgacaca acatactgta
     2221 acgcgcggtg atatcgcgca tcgggttttc cagcaccttc agcgcataag ggatcgccat
     2281 taacgcgttg gtgaaaatca caatgccgtc agcagattgc ggcaggccga tagtgttgtt
     2341 gagcagtaaa aagaagcccg tagccagcac aatccccggc atggcgagga tcaacatgcc
     2401 gctcatctcc agcgcctgac ccgccagcat tttctgccgc gcccgcagtt cgcgactgct
     2461 ccatagcagc atcatggtca gcactacgca taatacgcct gccgccagcg caatacgcaa
     2521 cgaggtccac agcgcctgcc acagcaccgg ctgcgccagc acttccggca actggcgatt
     2581 tatcccatcg acgagcaccg ccagtaacgg tggcagcaac agcaacagcg ccagcacaat
     2641 taacaccgtg tcgcaaatgc ggctatgcag acgatcgtcc gggtcgcgcc agccttgcag
     2701 cagtgtggta ccaggcgcaa tggccttact caatcgctga ctcaacagca ccagcccgag
     2761 gcagcacacc atctggatca gcgccagcat tgccgcgcgg gcaggatcgt agtcgtaact
     2821 cagcgcctga tagattgcca gctcgatagt ggtcgcctgc ggaccgccgc ccagcgatag
     2881 cacggtggcg aagctggcga aacagagcat aaagattagt gcggcaaccg gcggaatttg
     2941 tcgccgtaac cacggccatt cgacgaagcg gaagaagtga cagccgcgca tcccaagctg
     3001 ggcggcaagc tgacgttgtt cgccgggaat gttttccagt gcctggagta ataaacggct
     3061 agccatcggc agattaaaaa acacatgggc cagcaaaatg ccttgcaggc cgtagggcga
     3121 aaaggtccac tccagaccaa gtgattgcca gagcgaggcc agccagccct ggcgaccata
     3181 gacgctaaga atgccgaaaa cggcaaccag caccgggagg atcaaggtca ttgcgcacag
     3241 acgcaacagc atctgtcgcc caggaaagcg cctgcgatag agggcgcggg cgaggaatat
     3301 tgcgggtacg acagagagca gcgccgagag aaacgcctgc cagaaggaga agcgcaccac
     3361 atgccacagg tagctgtcct gccagactga cacccagtca ccctgcggcg cgttccacca
     3421 cagggcgaga aacgccgcca gtgcaaccgc taccaccagc gtggcggcgc ttacgcctgg
     3481 aattaaccag ccgggaatta acggctgacg gcgcgttgcc attcgctaat ccatgcctga
     3541 cgttgtgccg ccacttcggc gggtgtgaac tctagtgtgg tggctggttt ggtcaattgt
     3601 tcaaatcctg caggcagagt gacgtttgct accggataca tccagttgcc ggttgggatc
     3661 gcattctgaa aagccggaga aaccataaac tggaggaatt tttgcgccag ctctggctgc
     3721 ttgctggcag cggtgcgggc ggcaacttcc acctgcagat agtgaccttc gataaagttt
     3781 gtggcggcgt agttatcttt cttctcttcg agaatgtgat aagccggaga ggtggtgtaa
     3841 ctcagtacca gatcgctttc gccttttaaa aacaggccgt aggcttcgct ccagcctttg
     3901 gtgaccgtga cagttttctt cgccagtttc tgccaggctt gcggggcgtt atcgccatag
     3961 actttttgca tccatagcaa cagacccagc cccggtgtac tggtacgtgg atcttcataa
     4021 atcacccgcc agttttgatc gctctcaacc agttctttca ggctttgcgg cggatttttc
     4081 agtttgttct tgtcatagac gaaggcgaaa tagccgtaat catacggtac gaaagtgtca
     4141 ttattccagc cgccaggaac gttaacggct tccgctgcca caccgctttt agcaaacagc
     4201 cctgttttgc tggcggcgtc taacagattg ttatccagcc ccagcaccac atcggctttg
     4261 ctgtttttgc cttccatccg tagacggttg agcagcgaaa cgccatcttc cagcgccacc
     4321 agtttcagtt cgcaattaca gtctgcttca aaggcttttt tgactaccgg accaggtccc
     4381 cagtcagcgg caaaggaatc gtaggtataa acagttagaa caggtttagc gaaaacaggc
     4441 gctgtgcaca gcaacagcag gggaagacat ttttttaaca ctttgcacct cacaaaagag
     4501 tggcaaagga cttgagaagg agcctcaaat cccttcgccg gcgttatccg gatcaggttc
     4561 gacgggtatt ttctcagcgc acgcgtctgc gtggcacccc gttgagaacg gcgttagtgt
     4621 agtgattttg ttatcaacca gcaatcatgg atccggtggt gcaaaccacg ctgatttaaa
     4681 atcgaaccag ccgagggtat tcatacgcag gccgcgcata ctgcgttgcc cctgaatgat
     4741 cagccagtgg tggataagcg gcaccattgc tttgctggcg accagttgct ggcaccagtt
     4801 cgccaggttt atttcgccat tgcgccagcg ggcggcgtcg gcttgccagt cgatcggaat
     4861 acagtgttgt agcagcggca cctcgcacag gtgcgcgaat agcgaaaaat ccagcggcag
     4921 cgtaaagttg gcgctgttaa gccagatatc gctctcgatc tctccttcat gccactgatc
     4981 gtagctgatc tctttgattt ccagcgtgac ctggtgactt gccagaatct gctgcatgat
     5041 cccggcaatc accctatgct cactgtgatc ctgataaaag gtcagggtga ggctttccag
     5101 accagccggt ttttcgctct ttatggtgcg ggtatggtgc caacggggaa gcagcccata
     5161 ggccggaaac cacaactgct ggtactgttc ctcggcgaaa taaaccagat tggtcggcga
     5221 aagcacatag cttatccagt ctctgacttg ctgattcgcc ccgcgatggg tacggctgtc
     5281 gaacagtaaa tagtaacaac cttcttcaag gcggctttca atctcttttt cctcgtcttg
     5341 cggtcctttc agcatcaggc ctcccgcggg gtcatcagca atttccggca agacccagac
     5401 gttaacttcg tcgattaatg cccgataacc aaagaagtcg tcgaatgcct gaattttcag
     5461 ttgattgctg ctgttgcgga tcaccgcata aggacccgtt ccgatgggat ggctggcaaa
     5521 gttgctgagg gtttcccatt cgcgcggcag gatcatcgcc ggaacttgcc ccagcagtaa
     5581 cggtaaccag cggtccggtt gcgtgagatg gatatccagc gtccagggcg tcggcgacac
     5641 aatgtcagca atatgcgaat agagcggcag cgtattgatt cgttttaaag aggcgatcac
     5701 gtcgtccatt tccagttcac gaccatgatg aaaatggact cctggacgca aaaagaaacg
     5761 ccagtgaagc ggtaaaattt gctgccagtg gtgggcgatg tccgcttcca gttccccatt
     5821 ttcctcattt atgcgcgtta gcgaactgaa gatttgccgg gcgatatggg tttcggaacg
     5881 acgcaatgca ctgccgggca gcagattacg caacggacga tagtagagaa cgcgcaggat
     5941 gtgccgcccc tggcggaagc tgcgtcccag atgagaaacc aacatttgcc gcacagccgc
     6001 tttgtcgcca accagttgca ccagttgatc gatacgatcc tgctccagca ggtcctctgc
     6061 tcgctgttgc tgaagcgcca gcccggtata gaggaatgtc agacgcgagc gtttgccgcg
     6121 cccgacttcc gcttcccacg tcagccagcc gcgatcctgc atggtgttga gcagggtgcg
     6181 catatgacga cgcgagcagc tcaataacgc tgccagttcg ttgagcgtcg tgtcctgcga
     6241 tttaccctcg cagcattgcc acaggcggat gaactgttgt tgcagacgag cagatggcat
     6301 aaaaggggaa ctcctgcgca aaagacagca attttatttt ccctatatta agtcaataat
     6361 tcctaacgat gaagcaagga ggtgcctcat gcgtcagttt tatcagcact attttaccgc
     6421 gacagcgaag ttgtgctggt tgcgttggtt aagcgtccca caacgattaa ccatgcttga
     6481 agaactgatg caatgggagg ggagtcattc tgactactga tttgcggaca tcatgtgtga
     6541 ctgagtattg gtgttaatcg ccacgccagc agtgattatc tgctggcttt tttctttttg
     6601 gcgattgagt gccggacggt gtacaaacta acgcataaac gccggttgct tctcttcata
     6661 agacgcaatt gcgtcgtcgt gctgcagagt caggccgatg ctgtccagac cgttcatcat
     6721 gcagtggcgg cggaaggcat cgatggtaaa gcgataagtt ttctctcccg ctttcacctc
     6781 ttgcgcttcc aggtctacat caaaatgaat ccccggattg gcctgcacca gcgcaaacag
     6841 ttcgtccact tctgcgtcgc ttaatttcac cggcagcagc tggttgttaa agctgttgcc
     6901 gtagaagatg tcagcaaaac tcggtgcaat caccacttta aaaccgtaat cggtcaatgc
     6961 ccacggcgcg tgctcacgcg aggaaccgca gccgaagttt tctcgtgcca gcaaaatgga
     7021 agcgccctga tactgcggga agttcagcac gaagtccggg tttggctgtt ggcctttttc
     7081 atccagaaaa cgccagtcgt taaacagatg cgcgccaaaa cccgtacggg tcactttctg
     7141 caaaaactgt ttcgggatga ttgcatcggt atcgacattg gcggcatcca gcggaaccac
     7201 caggcctgtg tgtttgataa atttctctgc catggtgtgc tccttattta atgttgcgaa
     7261 tgtcggcgaa atgtccggtc acagcagcag cggcagccat tgccgggctg accagatggg
     7321 tgcgccctcc acgcccctgg cggccttcaa agttacggtt gctggtggag gcacaacgct
     7381 cacccggatt caggcgatcg ttgttcatcg ccagacacat tgagcaacct ggcaagcgcc
     7441 attcaaaacc ggcttcaata aagattttgt ccagaccttc cgcttccgcc tgggctttta
     7501 ccgggccaga gccgggaacc accagtgcct gcacgcctgg cgcgactttc cgtcctttgg
     7561 cgatttcagc tgccgcgcgt aaatcttcaa tacgcgagtt agtgcaggaa ccgataaaca
     7621 ctttgtcgat agccacttct gtcagcggaa tacccggttt cagccccata taggccagcg
     7681 ctttttctgc cgacgcgcgt tcaaccggat cggcaaacga agccggatcg ggaatgttgt
     7741 cgttcacgga aatcacctga cctggattgg tgccccaggt gacctgcggt gaaatttctt
     7801 ctgcctgcaa agtcacaacg gtatcgaaag ttgcgccttc gtcggtttgc agagttttcc
     7861 agtaggcaac ggcgtcgtcg aaatctttgc ctttcggcgc atgcagacgg cctttgacat
     7921 agttaaaggt agtttcgtcc ggtgcaacca gaccggcttt cgcgcccatt tcgattgcca
     7981 tattgcacag ggtcatacga ccttccatgc ttaaatcacg gattgcttcg ccgcaaaact
     8041 ccaccacatg cccggtgccg cctgcgctac cagttttacc gataatcgcc agcacgatat
     8101 ctttcgctgt aatgcccggc gcggctttgc cctggacttc aattttcatg gtcttcgcac
     8161 gaccctgttt cagggtttgc gttgccagta cgtgttcaac ttcggaagtg ccgataccaa
     8221 aggctagtgc gccaaacgcg ccgtgggtgg cggtatgcga gtcgccgcag acaatggtca
     8281 tccccggcaa tgtgacgccc tgttccggcc ccattacgtg gacgatcccc tgatacgggt
     8341 gattcaggtc atacagctcg acgccaaatt ctttgcagtt tttgatcagc tcctgcatct
     8401 ggatgcgcgc catttcaccg caggcattaa tgtctttggt ctgagtagag acgttgtgat
     8461 ccatagtggc gaaggttttg cctggctgac gtaccgggcg accgtgggcg cgcagaccat
     8521 cgaacgcctg cggtgaggtc acttcatgca ccaggtggcg gtcgatatat aacagcgggg
     8581 tttcgttttc ggcttcgtac actacgtgag cgtcgaacaa tttttcgtat aacgttttag
     8641 ccatgattac accccttcta ccacatagcg ggcaatgata tcgcccattt cattggtact
     8701 aacggcggca gcgccacggg ctaaatcccc ggtgcgaatg ccttcttcta atgcgcggtt
     8761 aatggcgcgt tcaatggcgc aagccgcatc atctgcatcc aggctgtagc gcagcagcag
     8821 tgccagcgac agaatttgcg caatcgggtt ggcgatgttt ttgcctgcga tatctggtgc
     8881 cgagccgccc gccggttcat acagaccaaa accttgctcg ttcaggctgg cggaaggcaa
     8941 catccccatt gagccagtga tcattgcgca ctcatcagac agaatgtcgc caaacaggtt
     9001 ggagcacagc aagacgtcaa actgtgatgg atctttaatc agctgcatgg tggcgttgtc
     9061 gatgtacata tgcgccagtt cgacgtccgg gtattccgtg gcgatctcgt taacgatctc
     9121 ccgccataaa atagaggatt gcagcacgtt ggctttgtcg attgaggtga ctttgtggcg
     9181 acgcttgcgg gcagattcaa atgcgatgcg ggcaatacgc tcgatctcaa aacggtggta
     9241 cacctcggta tcaaacgctt tttcatattg tccgctacct tcgcggcctt ttggctgacc
     9301 gaaatagatg ccgccggtca gttcgcgcac acacaggatg tcaaagccgt ttgcggcaat
     9361 gtcagcacgc agtgggcaga acgcttccag cccctgatac agttttgccg gacgcagatt
     9421 gctgaataat ttgaagtgtt tacgcaaagg cagcagtgcg ccgcgttctg gttgctggtc
     9481 tggcggcaaa tgttcccact tcggaccacc taccgagcca aacagcacgg catcggcttg
     9541 ctcacaacct tcaaccgtcg caggcggcag cggctgtccg tggttatcaa tggctgcgcc
     9601 gcctacatcg taatggctgg tggtgatacg catcgcaaat cggttgcgca cggcatccag
     9661 cactttcagc gcctgggtca tcacttccgg accaataccg tcccccggca atacggcaat
     9721 atggtaattc ttcgacatca cacggtttcc ttgttgtttt cgttgtgttg agctttgcgt
     9781 tgcaactctt tttcgacttc tgcggcacgc cagatattgt tcagtacgtg caccatggct
     9841 ttggcggagg actcgacaat atcggtggcc aggccgacgc cgtggaagcg gcgaccgttg
     9901 tagttggcga caatatccac ctgacccagc gcatctttac cgtggccttt ggcggtcagg
     9961 ctgtatttca ccagttcgac gttatagtca gtgatgcggt ttatcgcttg gtagacggca
    10021 tcgaccggac cgttaccgtt ggcggcttct gctttgactt cttcgccaca ggccagtttg
    10081 acggcggcag tggcaatatc gttagaacct gactgcacgc tgaagtaatc cagacggaaa
    10141 tgctccggct cttcttgctg cttaccgatg aaggccagcg cctccagatc gtaatcaaac
    10201 acctggcctt ttttgtccgc cagcttcagg aaagcgtcgt acaggttgtc taaattatat
    10261 tcactttctt tatatcccat ctcatccatg cgatgtttca ccgccgcacg ccccgaacga
    10321 gaggtcagat tcagctggat ttggttcaga ccaatagatt ctggtgtcat gatttcgtag
    10381 ttttcgcggt ttttcagtac accatcctgg tggataccgg aggagtgtgc gaatgcgccg
    10441 ctgccaacaa tggctttgtt tgccgggatc ggcatattac aaatctggct aactaactgg
    10501 ctggtgcgcc atatctcctg gtgattgatg gcggtgtgga cgttgagaat atccttacga
    10561 actttgatcg ccatgatgac ttcttcaagg gaacagttac cggcacgctc gccgatcccg
    10621 ttcattgcgc cttctacctg ccgtgctccg gcatgtaccg ccgccagtga gttgccgacc
    10681 gccaggccca aatcgtcgtg ggtatgtacg gaaataatgg ctttgtcgat gttgggtacg
    10741 cgttcataca ggccgctgat gattccggcg aactcaaatg gcatggtgta gcccacggtg
    10801 tccggaatgt tgatggtggt ggcaccggca ttaatcgccg cttcgaccac tcgcgccaga
    10861 tcggcaattg gggtgcgtcc ggcatcttcg caagaaaatt caacatcatc ggtgtaatta
    10921 cgggcgcgtt tcaccatata gatagcgcgt tcgatcactt cgtccagcgt gctgcgcagc
    10981 ttggtggcga tgtgcattgg cgaagtggca ataaaggtat gaatacggaa ggcttcggcg
    11041 actttcaggg attcggctgc cacgtcaata tctttttcca cgcagcgagc taacgcgcat
    11101 acacggctgt ttttaacctg gcgagcgatg gtctgcactg attcaaaatc acccggcgaa
    11161 gagacgggga aaccgacttc cataacgtca acacccatac gctcaagggc cagcgcaatt
    11221 tgcagttttt ctttcacact caagcttgcc tgtaacgcct gttcaccgtc gcgcaatgtg
    11281 gtatcgaaaa taatgacttg ctggctcatg gtttgggtcc ttgtctcttt ttgagcgcct
    11341 cgcttcgggc ataaaaaaac ccgcgcaatg gcgcgggttt ttgtttgact gcgtgctggc
    11401 ttaatgctgg atgccgctca ctcgtctacc gcgcaaaaaa gatgcgttta gtagtagtag
    11461 accgataaag cgaacgatgt gagtcattaa atcagctcca gatgaatgcg atatgctttt
    11521 agagttactg gatacaaaaa cggatgtcaa ccctgaagca ataaaaacgt cacgccattg
    11581 tgagttcggc atccgtaaaa ttagctaatt gtgctgcggt ggttaaaaca agcgatatta
    11641 atttctgctt aaccgccgat gcttttcatc ggttgacata tttcagaata attttttgca
    11701 tctaatcaac gagggaaagg gggataaaat gcacgcgtta caaaacctat cctgatgatt
    11761 tgtattgaat tatatgtttt gcgaattttt tgatattcat ttgctg
//
LOCUS       DADSZD010000132        11769 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.133, whole genome shotgun sequence.
ACCESSION   DADSZD010000132 DADSZD010000000
VERSION     DADSZD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.133"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            65..2242
                     /gene="iroN"
                     /locus_tag="J1Z28_003350"
     CDS             65..2242
                     /gene="iroN"
                     /locus_tag="J1Z28_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore salmochelin receptor IroN"
                     /protein_id="HBA7718157.1"
                     /translation="MRINKILWSLTVLLVGLNSQVSVAKSSDEDNDETLVVEATAEQV
                     LKQQPGVSVITSEDIKKTPPVNDLSDIIRKMPGVNLTGNSASGTRGNNRQIDIRGMGP
                     ENTLILIDGVPVTSRNSVRYSWRGERDTRGDTNWVPPEQVERIEVIRGPAAARYGSGA
                     AGGVVNIITKRPTNDWHGSLSLYTNQPESSDEGATRRANFSLSGPLAGDALTTRLYGN
                     LNKTDADSWDINSPVGAKNAAGHEGVRNKDINGVVSWKLNPQQILDFEAGYSRQGNIY
                     AGDTQNSSSSAVTESLAKSGKETNRLYRQNYGITHNGIWDWGQSRFGVYYEKTNNTRM
                     NEGLSGGGEGRILAGEKFTTNRLSSWRTSGELNIPLNVMVDQTLTVGAEWNRDKLDDP
                     SSTSLTVNDRDISGISGSAADRSSENHSQISALYIENNIEPVPGTNIIPGLRFDYLSD
                     SGGNFSPSLNLSQELGDYFKVKAGVARTFKAPNLYQSSEGYLLYSKGNGCPKDITSGG
                     CYLIGNKDLDPEISVNKEIGLEFTWEDYHASVTYFRNDYQNKIVAGDNVIGQTASGAY
                     ILKWQNGGKALVDGIEASMSFPLVKDRLNWNTNATWMITSEQKDTGNPLSVIPKYTIN
                     NSLNWTINQAFSASVNWTLYGRQKPRTHAETRSEDTGGLSGKELGAYSLVGTNFNYDI
                     NKNLRLNVGVSNILNKQIFRSSEGANTYNEPGRAYYAGVTASF"
     gene            complement(2287..3243)
                     /gene="iroE"
                     /locus_tag="J1Z28_003351"
     CDS             complement(2287..3243)
                     /gene="iroE"
                     /locus_tag="J1Z28_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroE"
                     /protein_id="HBA7718158.1"
                     /translation="MYAREYRSTRPHKAIFFHLSCLTLICSAQVYAKPDMRPLGPNIA
                     DKGSVFYHFSATSFDSVDGTRHYRVWTAVPNTTAPASGYPILYMLDGNAVMDRLDDEL
                     LKQLSEKTPPVIVAVGYQTNLPFDLNSRAYDYTPAAESRKTDLHSGRFSRKSGGSNNF
                     RQLLETRIAPKVEQGLNIDRQRRGLWGHSYGGLFVLDSWLSSSYFRSYYSASPSLGRG
                     YDALLSRVTAVEPLQFCAKHLAIMEGSATQGDNRETHAVGVLSKIHTTLTILKDKGVN
                     AVFWDFPNLGHGPMFNASFRQALLDISGENANYTAGCHELSH"
     gene            complement(3328..4557)
                     /gene="iroD"
                     /locus_tag="J1Z28_003352"
     CDS             complement(3328..4557)
                     /gene="iroD"
                     /locus_tag="J1Z28_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore esterase IroD"
                     /protein_id="HBA7718159.1"
                     /translation="MLNMQQHPSAIASLRNQLAAGHIANLTDFWREAETLNVPLVTPV
                     EGAEDEREVTFLWRARHPLQGVYLRLNRVTDKEHVEKGMMSALPETDIWTLTLRLPAS
                     YCGSYSLLEIPPGTTAETIALSGGRFATLAGKADPLNKMPEINVRGNAKESVLTLDKA
                     PALSEWNGGFHTGQLLTSMRIIAGKSRQVRLYIPDVDISQPLGLVVLPDGETWFDHLG
                     VCAAIDAAINNRRIVPVAVLGIDNINEHERTEILGGRSKLIKDIAGHLLPMIRAEQPQ
                     RQWADRSRTVLAGQSLGGISALMGARYAPETFGLVLSHSPSMWWTPERTSRPGLFSET
                     DTSWVSEHLLSAPPQGVRISLCVGSLEGSTVPHVQQLHQRLITAGVESHCAIYTGGHD
                     YAWWRGALIDGIGLLQG"
     gene            complement(4661..8446)
                     /gene="iroC"
                     /locus_tag="J1Z28_003353"
     CDS             complement(4661..8446)
                     /gene="iroC"
                     /locus_tag="J1Z28_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin/enterobactin export ABC transporter
                     IroC"
                     /protein_id="HBA7718160.1"
                     /translation="MIIICQHPSRSHASITMRDRSVLAFLRNDPASGSYFTDGGGLMP
                     ANHTPTPAQSWIVRLARVCWERKKLSVIVVVASVSTILLAALTPLLTRQAVNDALAGN
                     PARLPWLACGLLLIAFFDFIGNYVRRGYAGILSLWVQHTLRGRVFDSIQKLDGAGQDA
                     LRTGQVISRTNSDLQQVHTLLQMCPVPLAVFTYYIAGIAVMLWMSPAMTLIVVCVLVC
                     LAITALRARRRVFAQTGLASDQLANLTEHIREVLAQISVVKSCVAEMRETHWLDRQSR
                     QIVRVRIGAVISQAMPGATMLALPVLGQIVLLCYGGWSVMHGRIDLGTFVAFASFLAM
                     LTGPTRVLASFLVIAQRTQASVERVFALIDTHSQMEDGTESINSQVVGLELENMSFDY
                     HHGDRHILSDISFSLRAGETVAVVGASGSGKSTLLMLLARFYDPCSGKIWLNTSEGRQ
                     NLRDIRLEALRRRVGIVFEDAFLFAGTVAENIAYGHPQATADDIRRAAAAAGASDFIN
                     ALPKGFDSLLTERGTNLSGGQRQRIALARALITAPDVLILDDTTSAVDAVTEAEINTA
                     LGRYAEEGHMLLVIARRRSTLQLASRVVVLDKGRMVDTGTPAELEARCPAFRALMTGD
                     SDFLATSHNSHNELWPAEPATQDDVTDTGDKGFVARMTRVPENAVQQALAGKGRKVTS
                     LLKPVAWMFVIAALLIALDSAAGVGVLILLQHGIDSGVAAGDMSTIGLCALLALCLVI
                     VGWCSYSLQTVFAARAAESVQHSVRLRSFSHMLRLGLPWHEKHADSRLTRMTVDVDSL
                     ARFLQNGLAGAATSLVTMFAIAATMFWLDPFLALTALSAVPVAALATMIYRRLSTPAY
                     AQARLEIGKVNSTLQEKVSGLRVVQSHGQQELEGARLRALSERFRATRVRAQKYLAVY
                     FPFLTFCTEASYAAVLLVGASQVAAGEMTAGVLAAFFLLLGQFYGPVQQLSGIVDAWQ
                     QATASGKHIDELLATEGTENLGSSSVLPVTGALHLDEVTFSYPDSHEPALNKLTLTIP
                     EGMVVAVVGRSGAGKSTLIKLIAGLYFPTHGNIRIGVQMLDDASLTEYRRQIGLVDQD
                     VALFSSDIAENIRYSRPSATNEDVEIASQRAGLYEMVCNLPQGFRTPVNNSGADLSAG
                     QRQLIALARAQLANAHILLLDEATSCLDRTSEERLMSSLTDVVHAGKHSALIVAHRLT
                     TAQRCDLIAVIDKGLLAEYGTHEQLLSAGGLYTRLWHDSVSSTALHRQHNMKEETPG"
     gene            complement(8460..9575)
                     /gene="iroB"
                     /locus_tag="J1Z28_003354"
     CDS             complement(8460..9575)
                     /gene="iroB"
                     /locus_tag="J1Z28_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="salmochelin biosynthesis C-glycosyltransferase
                     IroB"
                     /protein_id="HBA7718161.1"
                     /translation="MRILFVGPPLYGLLYPVLSLAQAFRVNGHEVLIASGGQFAQKAA
                     EAGLVVFDAAPGLDSEAGYRHHEAQRKKSNIGTQMGNFSFFSEEMADHLVEFAGHWRP
                     DLIIYPPLGVIGPLIAAKYDIPVVMQTVGFGHTPWHIRGVTRSLTDAYRRHNVGATPR
                     DMAWIDVTPPSMSILENDGEPIIPMQYVPYNGGAVWEPWWERRPERKRLLVSLGTVKP
                     MVDGLDLIAWVMDSASEVDAEIILHISANARSDLRSLPSNVRLVDWIPMGVFLNGADG
                     FIHHGGAGNTLTALHAGIPQIVFGLGADRPVNARVVAERGCGIIPGEVGLSSNMINAF
                     LNNRSLRKASEEVAAEMAAQPCPGEVAKSLITMVQKG"
     gene            complement(10400..11252)
                     /locus_tag="J1Z28_003355"
                     /pseudo
     CDS             complement(10400..11252)
                     /locus_tag="J1Z28_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707076.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         2792 a         3146 c         3141 g         2690 t
ORIGIN      
        1 atctggcaag gatgtgagct taacgatcaa acattttaag ttaggcattt attagggaat
       61 aggtatgaga attaacaaaa tcctctggtc gctaactgtg ctcctggttg ggttgaatag
      121 ccaggtatca gtagccaaat cctccgacga ggataatgac gagactctgg tggtggaagc
      181 caccgctgag caggtattaa aacagcagcc gggcgtgtcg gttattacca gcgaggatat
      241 taaaaagacc cctccggtaa acgacctttc agatattatt cgtaaaatgc ctggcgtcaa
      301 tcttaccggc aatagcgcct cgggcacacg cggtaataac cgccagatcg atattcgtgg
      361 tatggggccg gaaaacacct taattttgat tgatggtgta ccggtgacgt cacgtaactc
      421 cgtgcgttat agctggcgtg gagagcgtga tacccgcggt gacaccaact gggtgccacc
      481 ggaacaggtt gagcgtattg aagtgatccg cggccctgcg gcggcgcgct acggttcggg
      541 ggcagccggg ggggtggtga acatcattac caaacgtccc accaacgact ggcacggttc
      601 gctgtcgtta tataccaacc agccggaaag tagcgatgag ggcgctacgc gtcgcgccaa
      661 tttcagcctt agtgggcctc tggctggtga tgctcttacc acgcgtttgt atggtaacct
      721 gaataaaacg gatgctgaca gttgggatat taattctccg gtcggtgcga aaaacgcagc
      781 cgggcatgaa ggggtacgta acaaagatat taacggcgtt gtctcgtgga aattaaatcc
      841 gcagcagatt ctcgatttcg aagccggata tagccgccag gggaatatct atgcgggcga
      901 tacgcaaaac agttcttcca gtgcagttac cgaaagcctg gcaaaatccg gcaaagagac
      961 gaaccgcctg taccgacaga attatggcat tacgcataat ggtatctggg actggggaca
     1021 aagtcgcttt ggtgtttatt acgagaaaac caataatacc cgcatgaatg aaggattatc
     1081 cggcggtggt gaagggcgta ttttagcggg tgaaaagttt acgaccaatc gccttagttc
     1141 ctggcgaacc agcggtgagc ttaatattcc tttgaatgtg atggttgatc aaacgctgac
     1201 cgttggtgca gagtggaacc gcgataagct cgatgatcct tcctctacca gcctgacggt
     1261 gaatgacaga gatatcagcg gtatttctgg ctctgctgcg gatcgcagca gtgaaaatca
     1321 ttctcaaatc agtgcgctgt atattgaaaa taacattgag ccggttccgg gcacgaatat
     1381 cattcccggc ctgcgctttg attatctcag cgactccggc gggaacttca gccccagtct
     1441 gaatctttcg caggaattgg gcgattattt caaagtcaaa gcaggggttg cccgaacctt
     1501 taaagcccca aacctgtatc aatccagtga aggctatctg ctctactcga aaggcaatgg
     1561 ctgtccaaaa gatattacat caggcgggtg ctacctgatc ggtaataaag atctcgatcc
     1621 ggaaatcagc gtcaataaag aaattgggct ggagttcacc tgggaagatt accatgcaag
     1681 tgtgacctac ttccgcaatg attaccagaa taagatcgtg gccggggata acgttatcgg
     1741 gcaaaccgct tcaggcgcat atatcctcaa gtggcagaat ggcgggaaag ctctggtgga
     1801 cggtatcgaa gccagtatgt ctttcccact ggtgaaagat cgtctgaact ggaataccaa
     1861 tgccacatgg atgatcactt cggagcaaaa agacaccggt aatcctctgt cggtcatccc
     1921 gaaatatact atcaataact cgcttaactg gaccatcaac caggcgtttt ctgccagcgt
     1981 caactggacg ttatatggca gacaaaaacc gcgtactcat gcggaaaccc gcagtgaaga
     2041 tactggcggt ctgtcaggta aagagctggg cgcttattca ctggtgggga cgaacttcaa
     2101 ttacgatatt aataaaaatc tgcgtcttaa tgtcggcgtc agtaatatcc tcaataaaca
     2161 gatcttccga tcttctgaag gggcgaatac ctataacgag ccaggccggg cttattatgc
     2221 cggagttact gcatcattct gatcgtagtg tttcaggcgc actgcccgcg taaaagcggg
     2281 cagtgtttag tggcttaact catgacaacc tgctgtgtaa tttgcgtttt caccactgat
     2341 atctaacagt gcctggcgaa aggatgcatt gaacatcggc ccgtgtccca ggttggggaa
     2401 atcccaaaat acggcattga cgcctttatc tttcagtata gtgagggtgg tatgaatttt
     2461 cgacagcacc ccgacagcat gcgtttcccg gttatcaccc tgtgtcgccg agccttccat
     2521 tatcgccagg tgtttggcgc agaattgcag aggctcaacc gccgtaacgc ggcttagcaa
     2581 agcatcataa cctctgccca acgacgggct ggcgctgtag tacgaccgga agtaagagga
     2641 ggacagccag gaatctagca cgaagaggcc gccgtaggag tgcccccata agccgcggcg
     2701 ttgccgatcg atattcagtc cctgttccac ttttggggca atacgcgttt ccagtaactg
     2761 gcggaagttg ttgctgccac cactcttacg gctaaaacgc cctgagtgga gatctgtttt
     2821 tctgctttct gctgctggcg tatagtcgta agccctgctg ttgagatcga aagggaggtt
     2881 ggtctgatac ccgacagcca cgatcactgg cggtgttttt tctgacaatt gtttgagcag
     2941 ttcgtcatcc aggcggtcca taactgcgtt accgtcaagc atatataaaa tcgggtaacc
     3001 cgatgccggt gcggttgtat tcggcacggc cgtccatacc cgataatggc gtgtgccatc
     3061 gacagagtcg aaagaggtgg cgctgaaatg gtaaaacacg gagcctttat cggctatatt
     3121 cggccccagt ggccgcatat ccggcttcgc ataaacttgc gcactacaga taagggtaag
     3181 gcaagaaaga tgaaagaaaa tcgctttatg cgggcgtgtt gagcgatact cgcgggcata
     3241 catctgatga ttattccttt tccgcaggtg acaatttgat ttattcagga agtctgtacc
     3301 gtttcctgaa agtgtttgtg ggtcaactca accctgtagt aaaccaatcc cgtcaatcag
     3361 tgcaccgcgc caccatgcgt aatcgtgacc accggtgtag attgcgcaat ggctttcgac
     3421 gccagcggta atcagccgct ggtgaagttg ctgaacgtga ggcactgtcg aaccttccag
     3481 cgatcccacg cacaggctga tacgcacgcc ctgcggtggg gcagaaagca gatgctcact
     3541 cacccatgag gtatcggttt cgctgaacaa gcctggtcga ctggttcttt ctggcgtcca
     3601 ccacattgaa ggagagtggc tgagcaccag accgaacgtt tccggtgcgt aacgagcccc
     3661 cattagtgca ctgatcccgc cgaggctctg cccggccagc actgtgcgcg aacggtctgc
     3721 ccactgacgc tgcggttgtt cagcgcggat catcggcagc agatgtcctg cgatatcctt
     3781 tatcagcttg ctgcgcccgc cgagtatctc agtgcgttca tgttcattaa tgttgtcaat
     3841 gcccagtaca gccacgggca cgatgcgcct gttatttatg gcggcgtcaa ttgccgcgca
     3901 tacgccaagg tgatcaaacc aggtttcacc atcgggcagc acgaccagcc cgaggggctg
     3961 agaaatatca acgtccggaa tatagagccg aacctggcga gatttcccgg cgataatgcg
     4021 catggaggta agcagttgtc cggtgtggaa gccgccgttc cattccgaca gggcgggagc
     4081 tttatcaagt gtcagcactg attcctttgc gtttccccga acgttgatct ccggcatttt
     4141 gtttagcgga tcggcctttc cggcgagggt ggcaaaacga cctccggaca gtgcaatcgt
     4201 ctcagccgta gtgccggggg ggatttccag cagcgaatag gagccgcagt aacttgcggg
     4261 taaacgcagt gtcagtgtcc agatatccgt ttcgggaagg gcgctcatca ttcctttttc
     4321 tacgtgctct ttatccgtca cccggttcag acgcagataa acgccctgca gaggatgtcg
     4381 ggcgcgccac agaaaggtca cttctcgttc atcttccgct ccttcgactg gcgtcacaag
     4441 aggaacattc agcgtctcag cttcgcgcca gaagtcagta aggttagcaa tgtggcccgc
     4501 tgccagttgg ttgcgcaggc tggcgatagc agagggatgt tgttgcatgt tcagcattca
     4561 tactctttaa aaattaagga tgtcagtata aataattaaa gcggcttttc acggtaccgc
     4621 cgcttctgac agtgttttta atacattacg tgtccagtaa ctatcccggg gtttcctcct
     4681 tcatgttgtg ctggcgatgg agcgcagtac tgctgacgct gtcatgccat aagcgggtat
     4741 agaggccgcc cgcagataac agctgttcgt gggttccgta ttccgcaagt aatcccttat
     4801 caataacggc aatcagatcg cagcgttgcg cggtagtcag acgatgtgca acaatcagcg
     4861 ccgagtgctt cccggcatgc acgacatctg ttaacgatga catcagtcgt tcttcggatg
     4921 tgcgatccag acatgacgtg gcttcgtcga gcagcaggat gtgagcattc gccagttgcg
     4981 cgcgggccag cgcaatcaac tggcgctgac ctgcggacag atcggctccg ctgttattca
     5041 ccggtgtccg gaatccctgc ggcagattgc acaccatctc atacagccct gcccgctgtg
     5101 aggcaatttc aacgtcttca ttggtggcgg atggccgtga ataacgaatg ttttctgcaa
     5161 tatcactact aaacagtgct acatcctgat cgacaagccc aatctggcga cgatactcag
     5221 tgagcgaggc atcatcgagc atttgcacac cgattctgat gttgccgtgc gtggggaaat
     5281 acaacccagc aatcagctta atcagcgtcg acttacccgc accgctgcga ccgacgaccg
     5341 cgacaaccat tccctcaggg atcgtcaggg taagtttgtt cagagctggc tcgtgactgt
     5401 cgggataact gaacgtgacc tcatcaagat gcagtgcacc ggtgacaggg aggaccgaag
     5461 aggacccgag gttctcagtg ccttctgtcg ccagtagttc atcaatatgt ttgccgctgg
     5521 ctgtcgcctg ctgccaggcg tcgacaatcc ctgataactg ttgcactggc ccatagaatt
     5581 gccccagcaa caggaagaaa gccgccagta ccccggcagt catttctcca gcggcgacct
     5641 gcgaagctcc cactaacaga acggcggcat aggaggcctc ggtgcagaat gtcaggaacg
     5701 gaaaatagac tgcaaggtat ttttgtgctc gcacacgggt tgcgcggaag cgctccgata
     5761 acgcgcgcag ccgggcgccc tccagttcct gctgaccatg cgattggacg acacgcaggc
     5821 cggagacttt ttcctgcagg gtgctgttga ctttgcctat ttccagccgt gcctgtgcat
     5881 aagcaggggt actgaggcgg cgataaatca tggttgccag tgcggccact ggcactgcgc
     5941 ttaatgccgt cagcgccagg aacgggtcga gccagaacat ggtggcggcg attgcgaaca
     6001 tcgtcaccag actggtggcc gcaccggcaa ggccgttttg cagaaagcgg gcgagagagt
     6061 ccacatcaac ggtcatgcgg gtcagacgcg aatcggcatg cttttcatgc caggggagtc
     6121 caagacgcag catatggctg aagctgcgca agcgcaccga atgctggact gattccgccg
     6181 ctctggcggc gaagaccgtc tgcagagaat aactgcacca gcccacaatg accaggcaca
     6241 gggcgagcag ggcacagagg ccgatggtcg acatatcgcc tgcggcgaca ccggagtcaa
     6301 tgccgtgctg caacagtatc agcaccccta cgcctgccgc agaatcgagt gcgatcagca
     6361 gagcggcgat gacgaacatc cacgccacag gcttcagtag tgacgtgact ttgcgacctt
     6421 taccggccag cgcctgctgt actgcatttt ccggtacgcg agtcatacgg gcgacaaaac
     6481 ctttatcccc cgtatccgtt acatcgtctt gtgtcgctgg ttcagccggc cacaattcgt
     6541 tgtggctatt gtgggacgtg gccagaaaat cgctgtcgcc ggtcatcagt gcgcggaacg
     6601 ccggacagcg cgcttcaagt tctgccgggg ttccggtatc caccatacgg cccttatcca
     6661 gcaccacaac ccggctggct agctgaagcg ttgaacgccg tcgggcaatc accagcagca
     6721 tatgcccttc ttcagcatag cgacccagcg cggtattaat ctccgcttcc gtaacagcat
     6781 caaccgctga ggtggtatca tccaggatta acacgtccgg tgcagtaatg agcgcccgcg
     6841 ccagcgctat tcgctgcctc tgcccgccgg aaagattcgt accccgttcg gttaacaggc
     6901 tatcgaagcc tttcggcagg gcgttgataa aatcgctggc tcctgcagca gctgccgcac
     6961 ggcgaatgtc gtccgccgtt gcctgagggt ggccataggc gatattttcc gccaccgtac
     7021 cggcaaacag aaaagcatct tcaaatacga tgcctacccg gcgacgaagc gcctccagtc
     7081 tgatatcgcg aagattttgt cggccttcgc tggtgttgag ccatatcttt ccggagcagg
     7141 gatcataaaa acgcgccagc agcatcaaca gggtcgattt tcctgaaccc gatgcgccca
     7201 ccaccgccac ggtttcaccg gcgcgcaggg aaaaggagat atcgctgagg atatgtctgt
     7261 cgccatggtg gtagtcaaag ctcatattct ccagttccag tccgacaacc tgactgttaa
     7321 tcgactccgt cccgtcctcc atctgtgaat gggtgtcgat cagtgcaaac acccgctcca
     7381 cggacgcctg agtgcgctgt gcgataacca gaaacgatgc cagtacgcgg gttggcccgg
     7441 tcagcatcgc gaggaagctg gcgaatgcaa cgaaggtacc gagatcgatc cgcccgtgca
     7501 tgaccgacca cccgccgtag cacagcagga cgatttgccc gagcaccggt agcgccagca
     7561 tggtggcccc aggcatcgcc tgcgagataa ccgcaccgat gcgtacacgc acaatctgcc
     7621 gcgactgcct atcgagccag tgcgtttcac gcatctctgc cacacaggat tttaccactg
     7681 agatctgtgc cagcacctcg cgtatatgtt cggtgagatt cgccagttgg tccgaggcca
     7741 gcccggtttg cgcgaagacc ctacgacgcg cacgaagcgc ggtgatcgcc aggcatacca
     7801 gtacgcacac gacgataagc gtcatggcag gggacatcca cagcatcacg gcaatgccgg
     7861 caatgtaata agtgaacact gccagcggca ccgggcacat ctgcagcagg gtatgcacct
     7921 gctgcagatc gctgttggtc cgtgaaatca cctgcccggt gcgcagcgca tcctggcctg
     7981 cgccgtcaag tttctgaata ctgtcgaata cccgtcctct gagggtatgc tgcacccaga
     8041 gtgagagaat cccggcataa ccacggcgca catagttacc gatgaaatca aaaaaagcga
     8101 tcaacagtaa cccgcaggcc agccacggca ggcgggccgg attgcccgcc agcgcgtcat
     8161 tcacagcctg tcttgtcagc agtggcgtca gcgcagccag caaaatagtc gatactgacg
     8221 ctaccaccac gatgacacta agtttcttac gttcccagca cacgcgtgcc aggcgaacta
     8281 tccatgactg tgccggtgtg ggagtgtgat tcgcaggcat taagcctcct ccgtctgtaa
     8341 aatacgatcc actggccgga tcgttccgca aaaaagccag cacgcttcgg tcccgcattg
     8401 ttatggatgc atggctcctg gatgggtgct ggcatatgat tatcatgaag gtgtagtggc
     8461 taccctttct gtaccattgt gatcaggctt ttcgccactt cacctgggca gggttgcgcc
     8521 gccatttccg ctgctacctc ttcagaggct ttgcgaagtg agcgattgtt gaggaaagca
     8581 ttgatcatgt tactcgacag gccgacctcg ccggggataa tcccgcagcc gcgttcggca
     8641 acaacgcggg catttaccgg gcgatcggct cccaggccga agacgatttg tggaatacca
     8701 gcgtgtagcg ctgtcagggt attgcctgct ccgccatgat gaatgaaacc atctgcgccg
     8761 ttgaggaaga cacccatggg tatccagtca accaggcgaa catttgacgg cagcgagcgc
     8821 aaatcggacc gcgcattggc tgaaatatgc aggatgatct cggcatcgac ctcactggca
     8881 gaatccataa cccaggcgat taggtcaagg ccgtcaacca tcggtttgac agtgccgaga
     8941 ctcaccagca gacgtttacg ctcgggcctt ctctcccacc atggttccca caccgcgcca
     9001 ccgttatacg ggacgtactg catcggaata atgggttcac cgtcgttttc cagaatgctc
     9061 atactcggcg gtgttacgtc gatccaggcc atatcgcgtg gtgtggcccc gacattgtgg
     9121 cgacgatagg cgtccgtgag cgaacgcgtt acacccctga tatgccaggg ggtatggccg
     9181 aagcctacgg tttgcatcac gaccggtata tcatatttgg cggcaatcag cggcccgatg
     9241 actccgagcg gagggtagat gatgaggtca ggccgccagt gtccggcaaa ctccaccaga
     9301 tggtcggcca tttcttcgct gaagaatgag aagttgccca tttgggttcc gatgttactt
     9361 tttttccgct gtgcctcatg gtggcggtaa ccagcttcag aatccagacc gggggcggcg
     9421 tcaaacacca ccaacccggc ctctgccgct ttttgtgcga attgtcctcc gctggcgatc
     9481 agtacttcat gaccgttaac gcgaaatgcc tgcgccaggg acagcacagg atacaacagc
     9541 ccatagagag gcgggcctac aaacaaaata cgcatgagaa aatccctctc cgcttgacga
     9601 tctaaatcag gcagtcgacg cactcatatg ccagcagcag gacacgactt ttcgttaaac
     9661 ctgctttacc agatgctgcg ccatttgtgc ttcaccagcg ttatggacag atcagtcaga
     9721 gacttttccg tgacatacac aggtgaacag ggaactccgt atccccactt ccattaacgc
     9781 ttacgcgcca ttgtcctgtt gaacagccca aacggcgtcg gacagctacg ccgggatgtt
     9841 ctgacgcatt tgtgcagtgt tagaccgaca atgaatctgg aaagatccgt gactttttag
     9901 acacacggct tgcatcatcc tgatagtctc gccttcgctg gcctgtaagt aaaatggcct
     9961 tgtactggcg atgcttgttc cctttcaacc ttaccagaga caattttaac gcaaatgcta
    10021 atgataataa ttaccaatat cattaggcta ttgcaacggt aattaaaaac ttaatgaaaa
    10081 gggaagcact cataggacta tctggggtat ttaaattgaa acctacgatt ccattctctt
    10141 tcctgtatgt aatcctgacg cggggaatca aggggagaac gtccatggct atctttcagg
    10201 aagtgggact gcctaataac tttttgctga ttggatttat tgctggtgat tattatcttt
    10261 tattcgtatt tccgactaag ttgctttaca acattgagcc aagcatgaat cactgctgga
    10321 gtcactttac ccaaaccact gcggccagta gttgcgacag ttgaaaaatt gatcttaccc
    10381 acggaatgtg gacacagtgc taagtgagat tctggttttc aaattgttcc gggctgagac
    10441 cgccgcacgc attgtggcga cgccagcgat tgtaatcaca ctcgatataa ttaacactgt
    10501 cgtccgcatt atttctcggc tggcaaaacg ttctccgtgg atacagtcca ctttcaacga
    10561 gtaaaagaat ctttccacac agtcattgtc atagcagcag ccctttgcac tcatgctccc
    10621 ccgcagatta ttacgtttca gcaaggtctg ataatccgct gaacagtact ggccactacg
    10681 tccggagtga acaatgacat cttccggatg tttacgccgc cagagcgcca tttgcagtgc
    10741 atcgcaggca agttgtgccg tcatacgcgg cgacatcgac cagccaatga cggctcgcga
    10801 ccacagatca agcatcaccg ccagatacag ccagccttca tccgtacgta agtaggtgat
    10861 gtcacctgtc cacttctggt ttggccgctg gcgtaaaaat cctgtttctg cagattctct
    10921 gacacgggca agccgtgctc acggtaactg accggacaga acttgcgggt cgctttcgct
    10981 cgtagtccct gacgacgcag gctggcagca attgttttga tgttgtattc cggcaattcg
    11041 tcagcaagac gaggcgcacc ataacgttgt tttgcctcag tgaatgcttt acggacagca
    11101 gcatcacaga tgagccggaa ctgctggcgc gggcttattt gatggcgacg caggagctcg
    11161 gcataccagc ggctgcgggc aacccgaagt acacggcaca tggctttgac gctgaactca
    11221 gcccgatgtt tttcgataaa gacatacttc atttcaggcg cttcgcgaag tatgtcgcgg
    11281 ccttttggag ggaggccaat tcctcgtccc gttccgccag ttgtcgctta agacgggcaa
    11341 tttcaacaga catgtcttgc tcacgtttgc aagaggaatg tgaatttttc agcttgctac
    11401 gccaggcata aagctgagat taatacaggt tgagctcgcg tgcagcggca gcaacaccga
    11461 tgcgttctgc aagcttcagg gcttcattgc gaaattcagg cgagtgttgc ttacggggct
    11521 ttttgctggc tgatactggt ttagtcatga gtcacctctg attgaaagtg tactcactta
    11581 gtcccgtgtc cactattggc gggtaggatc attggtagga atcataagcg taatgacact
    11641 ggcggaagca gttaaccaat cgaagataaa aatgaaagcg aaagccactt cgcattgctc
    11701 aatgaattga atcatatcgt caggtgatta ccgatatttt taacaaaaat ataacaaaac
    11761 ggtaacgcg
//
LOCUS       DADSZD010000133        11734 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.134, whole genome shotgun sequence.
ACCESSION   DADSZD010000133 DADSZD010000000
VERSION     DADSZD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.134"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            121..1509
                     /locus_tag="J1Z28_003356"
     CDS             121..1509
                     /locus_tag="J1Z28_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK family nitrate/nitrite MFS transporter"
                     /protein_id="HBA7718162.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1592..5332
                     /locus_tag="J1Z28_003357"
     CDS             1592..5332
                     /locus_tag="J1Z28_003357"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBA7718163.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGTVCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCIGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAKGRPLIDTAIDASEVILALAPETNGHVAAKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5329..6873
                     /gene="narH"
                     /locus_tag="J1Z28_003358"
     CDS             5329..6873
                     /gene="narH"
                     /locus_tag="J1Z28_003358"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBA7718164.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQVDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYECQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6873..7568
                     /gene="narW"
                     /locus_tag="J1Z28_003359"
     CDS             6873..7568
                     /gene="narW"
                     /locus_tag="J1Z28_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBA7718165.1"
                     /translation="MQILKVIGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPNDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEESDDTHQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7565..8245
                     /gene="narI"
                     /locus_tag="J1Z28_003360"
     CDS             7565..8245
                     /gene="narI"
                     /locus_tag="J1Z28_003360"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBA7718166.1"
                     /translation="MIQYLNVFFYDIYPYLCGTVFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8325..9218
                     /locus_tag="J1Z28_003361"
     CDS             8325..9218
                     /locus_tag="J1Z28_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBA7718167.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMTPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKSETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIDVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIDF"
     gene            complement(9314..10159)
                     /gene="nhoA"
                     /locus_tag="J1Z28_003362"
     CDS             complement(9314..10159)
                     /gene="nhoA"
                     /locus_tag="J1Z28_003362"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBA7718168.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10334..10903
                     /locus_tag="J1Z28_003363"
     CDS             10334..10903
                     /locus_tag="J1Z28_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBA7718169.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10907..11134)
                     /gene="pptA"
                     /locus_tag="J1Z28_003364"
     CDS             complement(10907..11134)
                     /gene="pptA"
                     /locus_tag="J1Z28_003364"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBA7718170.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADINDVIIRHLNSKDSSISISL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11197..11734)
                     /locus_tag="J1Z28_003365"
                     /pseudo
     CDS             complement(11197..11734)
                     /locus_tag="J1Z28_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2758 a         3053 c         3056 g         2867 t
ORIGIN      
        1 ttatctattc ttctaatcag cacttcacct gattcatcct taattctaat aactggctat
       61 tgcgccttac ctcctgtttt gtcttttttt cattaccaat gtgtgcatgt gaggaacaat
      121 atggcactgc aaaatgagaa aaatagtcgt tatcttttac gcgactggaa accagaaaat
      181 ccggccttct gggaaaataa aggaaagcat attgcacgaa gaaacctctg gatatcagtc
      241 agttgtctac ttcttgcctt ctgtgtctgg atgctattta gcgcagttac tgttaatctc
      301 aataaaatcg gttttaattt cactaccgat caactctttt tattaaccgc attaccctcc
      361 gtttctggcg cattattgcg tgtcccctac tcctttatgg tgcctatatt tggtggacgc
      421 cgatggacgg tttttagtac tgcaatcctg attattcctt gcgtctggct cggaattgcc
      481 gtgcagaatc cgaatactcc ttttgggata tttatcgtta tcgctttgct atgcggtttt
      541 gcaggtgcga actttgcttc gagcatgggc aatatcagtt tcttcttccc aaaagccaaa
      601 caagggagcg cacttgggat taatggcgga ttaggaaact taggtgtaag tgtgatgcag
      661 ctggttgcac cgctggtcat ttttgtaccc gtatttgcct ttctcggcgt caatggcgta
      721 ccgcaggccg acggttcggt gatgtcgctg gcgaatgccg catggatttg ggtgccatta
      781 ctggcgattg ccacgatcgc cgcctggtca gggatgaatg atatcgccag ttcacgcgcg
      841 tcaattgccg accagctgcc agtgttacaa cgcctgcatc tctggctgct gagcctgctt
      901 taccttgcca ccttcggttc gtttatcggt ttttctgcgg gttttgccat gctggcgaaa
      961 actcagttcc ctgatgtgaa tattctgcgc ctggcgttct ttggcccatt tattggtgcc
     1021 atcgcgcggt cggttggtgg tgctatttcc gataaattcg gcggcgtgcg ggtgacgttg
     1081 atcaacttca tttttatggc gattttcagc gccctgctgt tccttacctt accgggcaca
     1141 ggctccggta atttcatcgc attttacgcc gtatttatgg ggttgtttct gaccgcaggt
     1201 ctgggaagtg gttctacttt ccagatgatc gccgtcatct ttcgccagat aaccatttat
     1261 cgggtgaaga tgaaaggcgg tagtgatgag caagctcaga gagaagccgt caccgaaacg
     1321 gcggcggctc tgggctttat ctcagccatt ggcgcagtgg gcggcttttt cattccacag
     1381 gcgtttggca tgtcgctcaa tatgaccggc tctccggtgg gcgcgatgaa agtgttttta
     1441 atcttctaca tcgtttgtgt gctgctgacc tggctggttt atggtcggcg gaagttcagc
     1501 caaaaataag agatcgatgc ctgacacgtc ttatcaggct acaaccgtgt cgcatcagat
     1561 cccaattcat tcctggagca ggagttatgt catgagtaaa cttttggatc gctttcgcta
     1621 cttcaaacaa aagggcgaaa cctttgccga tggtcacgga caggtgatgc atagcaaccg
     1681 cgactgggag gacagctatc gccagcgttg gcagttcgac aaaatcgtgc gttccaccca
     1741 cggtgttaac tgtacaggct cctgcagctg gaaaatctac gttaaaaatg gtctggtgac
     1801 ctgggaaatc caacagaccg actacccgcg cactcgccct gacctgccaa atcatgaacc
     1861 tcgcggctgc ccgcgtggcg caagttactc ctggtatctc tacagcgcta accgcctgaa
     1921 atacccgctc attcgtaaac gactgattga actgtggcgc gaagccctca aacaacacag
     1981 cgatccggta ctggcgtggg catcgattat gaacgatccg caaaagagcc tgagctacaa
     2041 acaagtgcgt ggacgcggtg gctttatccg ctccaactgg caggagctaa atcagctgat
     2101 tgccgccgct aacgtctgga ccatcaaaac ttacggcccg gatcgcgttg ccggtttctc
     2161 gccgattccg gcgatgtcga tggtttctta cgccgccgga acacgctatc tgtcgttgtt
     2221 aggtggcacc tgtttaagct tctacgactg gtattgcgac ctgccgcccg cctcgccgat
     2281 gacctggggc gagcaaaccg acgtaccgga atctgccgac tggtataact ccagttacat
     2341 catcgcctgg gggtctaacg taccgcagac acgtacgccg gacgcccatt tctttaccga
     2401 agtacgctac aaaggcacta aaaccatcgc cattacccct gactactctg aagtggccaa
     2461 attgtgcgac cagtggctgg caccgaaaca aggcactgat agtgccctgg caatggcaat
     2521 gggccatgtc attttaaaag agtttcatct cgataatccc agcgattatt ttatcaacta
     2581 ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg gaacctcgcg acgacggcag
     2641 ctacgttccc gggcgcatgg tccgcgcatc tgacctggtg gatggactgg gcgaaagcaa
     2701 taatccgcag tggaaaaccg tggcagttaa taccgcaggt gaattggtag tgccgaacgg
     2761 ttcgattggt tttcgctggg gagaaaaagg caaatggaac ctggaatcca ttgccgccgg
     2821 tacggaaacc gaattgtcgt taaccctgct cggtcaacat gacgccgttg caggcgtggc
     2881 cttcccctac tttggcggca ttgaaaatcc acattttcgc agcgtaaaac acaatcctgt
     2941 cctggtgcgc cagcttccgg tgaaaaatct gacgctcgcc gatggcactg tctgtccggt
     3001 ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc gatcgcgggc tggaagatga
     3061 aaacagcgcg aaagattacg ctgaaatcaa accttatacc cctgcctggg gcgagcaaat
     3121 taccggcgtt ccgcgccagt atattgaaac catcgcccgt gaatttgccg ataccgccca
     3181 taaaacgcat gggcgctcga tgattattct cggcgcaggt gttaaccact ggtatcacat
     3241 ggacatgaac taccgcggga tgatcaatat gctgatcttc tgcggttgtg tcgggcaaag
     3301 cggtggcggc tgggcacact atgtcggtca ggaaaaactg cgcccgcaaa ccggctggct
     3361 gcctctggcc tttgcgctcg actggaaccg gccaccgcgc cagatgaaca gcacctcgtt
     3421 tttctacaat cactccagcc agtggcgcta tgaaaaagtc accgcgcagg aattgctctc
     3481 accgctcgcc gatgccagta aatacagcgg tcacctgatt gatttcaacg ttcgcgccga
     3541 acgtatgggc tggctccctt ctgcgccgca gctggggcgt aacccgctct ccctgaaagc
     3601 tgaagccgac aaggccggat tatctcccac agaatttacc gcccaggcgc tgaaatcggg
     3661 cgatttacgt atggcctgcg aacaaccaga tagcggcagc aatcatccgc gtaacctgtt
     3721 tgtctggcgt tctaacctgc ttggctcctc cggcaaaggc cacgagtata tgcagaagta
     3781 tctgctgggg accgaaagcg ggattcaggg cgaggaactc ggtgccagcg acgggatcaa
     3841 accagaagaa gtcgagtggc aaactgcggc gattgaaggc aagctcgacc tgctggtgac
     3901 gctcgacttc cgcatgtcca gtacctgtct gttctccgat atcgttctgc ccaccgccac
     3961 ctggtacgaa aaagacgata tgaatacctc ggatatgcat ccatttattc atccgctttc
     4021 tgcggcggtc gatccggcct gggaatcacg cagcgactgg gaaatctaca aaggtatcgc
     4081 taaagccttt tcgcaagtgt gcatagggca tctcggcaaa gaaaccgacg tggtattaca
     4141 accactgctg catgactccc cggcagagct ttcacagccg tgtgaagtgc tcgactggcg
     4201 caaaggcgag tgcgatctga ttccaggcaa aaccgcgccg aatattgtgg cggtggagcg
     4261 cgactaccct gctacctatg aacgctttac ctcactcggg ccattgatgg acaaacttgg
     4321 caacggcggt aaagggattt cgtggaatac ccaggatgaa attgatttcc tcggtaaact
     4381 caattacacc aagcgtgatg gcccggcaaa ggggcgtccg ctgattgaca ccgccattga
     4441 cgcatcagaa gtgattctgg cactggcacc ggaaaccaac ggccatgttg cagccaaagc
     4501 gtggcaagcg ctgggcgaga tcaccggacg cgaacatacc catctggcgc tgcacaaaga
     4561 ggacgagaag attcgctttc gcgatattca ggcgcagcca cgtaaaatta tctccagccc
     4621 cacctggtct ggtctggaaa gcgatcacgt ctcctataac gcgggataca ccaacgttca
     4681 tgagttaatt ccgtggcgca cgctgtcggg acgccagcaa ctctatcagg atcatccgtg
     4741 gatgcgtgct tttggtgaaa gtctggtggc ttatcgcccg cctatcgaca cccgtagcgt
     4801 cagtgaaatg cgccagatac cgccaaacgg cttcccggaa aaagccctta acttcctgac
     4861 gccgcaccag aaatggggca ttcactcaac ctacagtgaa aacctgctaa tgctgacgct
     4921 ctctcgcggt ggaccgattg tctggatcag cgaaaccgat gcccgagaac tgaccattgt
     4981 cgataacgac tgggtggaag tatttaacgc caacggcgcg ctgactgccc gcgcggtggt
     5041 cagccaacgt gtaccgccgg gcatgaccat gatgtatcac gcccaggaac gcattatgaa
     5101 tattcctggt tcggaagtaa ctggcatgcg cggcggcatt cataactcgg ttacccgcgt
     5161 ttgcccgaaa ccaacgcata tgattggcgg ttacgcgcag ctggcctggg gctttaacta
     5221 ctacggcacc gtcggctcga accgcgacga attcattatg atccgcaaaa tgaagaacgt
     5281 taactggctg gatgatgaag gtcgcgatca ggttcaggag gcgaaaaaat gaaaatacgt
     5341 tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg gctgccatac ctgttcggtg
     5401 acctgtaaaa acgtctggac cggacgcgaa ggcatggagt acgcatggtt taacaacgtc
     5461 gaaaccaaac cgggcattgg ttatccgaaa aactgggaag atcaggatga gtggcaaggt
     5521 ggctgggtgc gtgatgtgaa tggcaagata cgcccgcgtc tgggcagcaa gatgggcgta
     5581 ataaccaaaa ttttcgccaa cccggtagtg ccgcaggttg acgattacta cgaacctttc
     5641 accttcgact acgaacattt gcatagcgcg ccggaaggca aacatattcc cacagcccgt
     5701 ccgcgttcac tgattgacgg caaacggatg gacaaagtga tctggggccc aaactgggaa
     5761 gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc gcaacttcga ggccatgcaa
     5821 aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt acctgccgcg cctgtgcgaa
     5881 cactgcctta accccagttg tgtagcgacc tgccccagcg gcgctatcta caaacgcgaa
     5941 gaagacggca ttgtactgat cgatcaggat aaatgccgtg gctggcgttt gtgcattagc
     6001 ggttgcccat acaaaaaaat ctacttcaac tggaaaagcg gtaagtcaga aaaatgtatt
     6061 ttctgttatc cgcgaattga gtccggtcag ccaacagtct gctccgaaac ctgcgtgggt
     6121 cgcattcgtt atcttggcgt gctgctctac gacgccgacc gcatcgagga agcggcgagc
     6181 accgagcgcg aagttgacct ctatgaatgc cagtgcgaag tgttcctcga tccgcacgat
     6241 ccctcggtga tcgaagaagc cctgaaacaa ggtattccac aaaacgtgat tgacgctgcc
     6301 cagcgttcgc ctgtctacaa aatggcgatg gactggaaac tggcgctacc gctgcaccct
     6361 gaataccgca ccctgccgat ggtctggtac gttcctccgc tgtcaccgat tcagtcctac
     6421 gctgatgcgg gaggtttgcc gaaaagcgaa ggcgtgctgc ccgccatcga aagcctgcgt
     6481 attccggtgc aatatctcgc caatatgttg agtgccggtg ataccggtcc ggtactgcgg
     6541 gcgctgaaac ggatgatggc gatgcgccac tatatgcgtt cacaaaccgt ggaaggcgtt
     6601 actgatactc gtgccatcga cgaagtaggt ctgagcgtcg cccaggtcga agagatgtat
     6661 cgttacctcg ccattgccaa ctatgaagat cgtttcgtca tcccgacaag ccatcgggaa
     6721 atggcgggcg atgccttcgc agaacgcaac ggctgcggtt ttacctttgg cgacggttgc
     6781 cacggttcgg acagcaaatt caacctgttc aacagtagcc gtatcgatgc catcaacatc
     6841 accgaagtgc gcgacaaagc ggagggcgaa taatgcagat cctcaaagtg atcggcctgt
     6901 tgatggagta cccagacgag ttgttgtggg agtgtaagga cgacgcgctg gcgcttatcc
     6961 gccgcgacgc gccgatgctc accgattttg cactcgacct gctcaacgcc ccgctgctgg
     7021 ataaacaggc cgaatggtgt gaagtgtttg accgcgggcg caccacgtcg ctgctgctgt
     7081 ttgaacatgt tcatgccgag tcccgcgatc gcggccaggc aatggtggac ctgctggcgg
     7141 agtatgaaaa ggtcggtctg caactgaatt gtagggaact gcccgattat ctaccgctgt
     7201 atctggagta tttaagcgta cttccgaacg atcaggcgaa agaaggatta ctcaacgttg
     7261 cgccgatcct cgccctgctt ggcggacgct taaaacaacg cgaggcaccg tggtacgcgt
     7321 tatttgacgc cctgttgcaa ctggcaggaa gccctctgtc aagtgacagt gtcacaaaac
     7381 agattcgcag tgaagagagt gacgacaccc accaggcgct ggatgcggtg tgggaagaag
     7441 aacaggtgaa gtttattgaa gataacgcca cggcgtgtga cagctcgccg cttaatcaat
     7501 atcagcgacg ctttagccag gatgtcgcgc cgcagtatgt cgacatcagt gcgggaggtg
     7561 ggaaatgatt cagtatctga acgtcttttt ttacgatatc tacccgtact tgtgtggcac
     7621 ggtttttatc cttggaagct ggctgcgcta cgattacggg cagtatacct ggcgcgccgc
     7681 ctccagccag atgctggata aacgcgggat ggtgatatgg tcgaacttgt tccatatcgg
     7741 cattctgggg atcttcttcg gtcatctgtt cggcatgtta acgccgcact ggatgtacgc
     7801 gtggtttctg ccggtggcgg taaaacagca gatggcgatg atcctcggcg gaatatgcgg
     7861 ggtattgacg ctgattggcg gtgcggggct actggtgcgt cgtttaacaa atccgcgtat
     7921 cagggcgacg tcttctggtg cggatatcct gatcctcgcc attttgttaa ttcagtgcat
     7981 tcttgggtta accaccattc ctttttcagc acaacatccg gacggcagtg aaatgatgaa
     8041 gctggtgggc tgggcgcaaa gcattgtcac tttccagggc ggtgcatcta cgcatcttga
     8101 tggtgtcgcc tttatattcc gcgttcatct ggtgcttgga atgaccatct tcctgctctt
     8161 cccgttcacc cgactggtgc atgtctggag cgcgccgttt gagtacttta ctcgtcggta
     8221 tcagattgtg cgttcgcggc gttaattctc acgcgggccg gatagccctt cgctctccgg
     8281 ccacttgcag gctatgatag ttctccagcc acaggagaac attaatgaaa ccgcaggttt
     8341 accacgtcga tgcttttacc tctcaaccgt ttcgcggcaa ttctgccggt gttgttttcc
     8401 ccgccgataa tctcagtgaa gcgcaaatgc agcttatcgc ccgcgagtta ggtcattcgg
     8461 aaactgcttt tctgctgcac agcgacgaca gcgatgtgcg catccgctac tttacgccaa
     8521 cggttgaagt tccgatttgt ggtcacgcga cagtagctgc gcactatgta cgtgcaaagg
     8581 tgttaggttt aggaaattgc acggtctggc aaacatcgct ggcaggaaaa catcgtgtga
     8641 ctatagaaaa gcaaaatgat gattatcgta tttcgctgga acaaggtact ccaggctttg
     8701 agccaccgct ggaaggtgaa acacgtgcag cgattatcaa cgcgcttcat ctcactgagg
     8761 acgatattct gccaggcttg ccaattcagg tggcaacaac aggacactca aaagtgatga
     8821 ctccactgaa gccggaagtg gatatcgacg ccctttcgcc tgaccttgct gcgctgaccg
     8881 caatcagcaa gcagattggc tgcaatggtt tcttcccgtt ccagatccgc ccaggcaaaa
     8941 gcgaaaccga tggtcgcatg ttctcgcctg cgattggtat tgtggaagat ccagtgaccg
     9001 ggaatgccaa tggcccgatg ggtgcatggt tagtacatca caacgtattg ccccacgatg
     9061 gcaacgtgtt gcgtgttaaa ggccatcagg ggcgcgcatt agggcgtgac ggcgtgattg
     9121 atgtgacggt gacaattcga gataaccaac cggagaaagt taccatttcc ggcacggcgg
     9181 tgattctatt ccatgctgaa tgggccattg atttttgatg ccgttgcctg atgcgcttcg
     9241 cttatcaggc ctacaaccgg actaatgtag gccggataag gcgtttacgc cacatccggc
     9301 aacctgacat aaattatttt cccgcctccg ggtgcgtatc aaacgccgcc atcaccagcg
     9361 ctaactcatc cacagtaaag ccatgtttcg catcatctac gcccagacca aactgttcct
     9421 gcatcacggc atataacgac gccacatctg gtagatttcg ctgctcaact gcgtgcccat
     9481 tttcataatg ggtaaaatga aaattggtca gcgttagctt gccaccgtcc ggcaaatggc
     9541 gacacatcag caaatgatgg cgaaaatgcg actgcggcca gtgcgccgac cagaaattgc
     9601 ccatcacata atcgctttgt tgctgctcgc agagatcaaa acggtacatc gactgccagt
     9661 gctgatgatg attaaactgc aatacccaat catcaccctc ttgcaacaac cgatactctc
     9721 cgtgtggcgt ggtttgcatg atatccggca ttaaacgaat cggcgcggtt agcgtctgcc
     9781 caccgaaacc gacatcagca atccattttt ccccttccag ttccaccaac agcaaacgat
     9841 gggtgcgcgg cggtaatgct ggcggatttg ataacactac gcgacctaac aagctgcgaa
     9901 cgttaaaccc cagctcgcgt aacacccgct caaacacgcc attctgctca aaacagtaac
     9961 cgccacgacg ggctatcacc agtttctctt ccagcgattg atcatcaagc tgtatttccc
    10021 tcggcagcaa aacgtcgaga ttttcaaacg gaatggtgca attgtgtttc aggtgcaatg
    10081 cacgcagcgt atcaatattg gccgcagcag ctcccaacca gttaatacgg gcaaaatagt
    10141 gattcagaat gggcgtcatc aggcgtttcc cttagtggtt ttctcaactt ataacctgga
    10201 atagacgccg cactggctat tttgtgcgat cgcggacatg catcacgttc gcaaatcgcc
    10261 aatcccgtta atttcgttac ttattgcgtt acgagctacg cttttaaaac acccttcata
    10321 gcgaggcgat atcgtgagcc aattcattcc cgtcgaatta caccatgcca gccgtctgtt
    10381 aaatcatggc ccaactgtca tgatcaccag tttcgatgag caatcccagc ggcgtaacat
    10441 tatggcagca gcctggtcga tgcccgtaga gtttgaaccg ccacgcgtgg cgattgtggt
    10501 agataaatcg acatggacca gggagttgat tgaacgtaac ggtaagtttg gcatcgttat
    10561 cccgggcgtt gcggcgacta actggacgtg ggcggtggga agtgtgtcgg gtcgtgaaga
    10621 agataaattt aattgctacg gcattccggt agtgagaggc ccggtgcttg gtttgccgct
    10681 ggtcgaggaa aaatgtctgg cgtggatgga gtgtcgcctg ctacctgcaa cttccgcgca
    10741 agaaaaatac gatacgctat ttggcgaagt ggtatcagca gcggcagacg cacgggtatt
    10801 tgtcgaaggc cgctggcagt ttgatgatga taagctcaat acgttgcatc atttaggtgc
    10861 tggcacgttt gttaccagtg gtaagcgagt tacggcaggt taagcattac atgctgtaac
    10921 cgggtttctt tatcaaagcc tccatttggg gcgcgatttc ggcatcccag atagcttgcc
    10981 aggattctgg ttgaatctgc tgtagagaaa tgcttatcga gctatcttta ctgttcagat
    11041 ggcgaataat aacgtcgtta atatctgcag caagcgctgc tttttgttgt tcgtccagtt
    11101 cacgcggaaa acatttaatg tcgatgtgcg gcatgattgt gctccttttg aaagatgtca
    11161 gtaaaattaa catacattgt gaatgtcagg gcaagattac gaaagcccac tccccgcaag
    11221 gactgacccc aggtagtttc tgtccatggc tgtttttcgc atcttacgtc ttaaccctgc
    11281 cttgaatatc ttttcattcg tcaaaatatt aatagcgatg taccgtctcc ctgaaaataa
    11341 ttcagcggcg ttccctcttc ttattttaca attgtcttca ttcattacca cgtccaggca
    11401 acagtgtagc ttattctcca cgtgccagtg atttcggagc gctgtgatga acttctctgc
    11461 ggttaaatca gcagaactga tataatatct gaccatcatt tcgggttctt tcttttgttc
    11521 tgctattatt gacttaaagg agactgctaa acataatttc ttcagccttt tccattcaaa
    11581 cgtgaaatca ataagttcat cagggacatc gcaaacaata tgaaaacgga tttcttcgcc
    11641 ggagtgtttt cccatcaatt gcgatgacgt ctttatcatc tgaagaatga ctgtcacgca
    11701 tccagttaat aaagcactcg tgaaatttcg cagg
//
LOCUS       DADSZD010000134        11733 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.135, whole genome shotgun sequence.
ACCESSION   DADSZD010000134 DADSZD010000000
VERSION     DADSZD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.135"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            211..1458
                     /gene="mdtA"
                     /locus_tag="J1Z28_003366"
     CDS             211..1458
                     /gene="mdtA"
                     /locus_tag="J1Z28_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBA7718171.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATAPEEKATSREYAKKGARS"
     gene            1458..4580
                     /gene="mdtB"
                     /locus_tag="J1Z28_003367"
     CDS             1458..4580
                     /gene="mdtB"
                     /locus_tag="J1Z28_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBA7718172.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIADS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            4581..7658
                     /gene="mdtC"
                     /locus_tag="J1Z28_003368"
     CDS             4581..7658
                     /gene="mdtC"
                     /locus_tag="J1Z28_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBA7718173.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQNVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLVISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            7659..9074
                     /locus_tag="J1Z28_003369"
     CDS             7659..9074
                     /locus_tag="J1Z28_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718174.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAITTLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGIVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMASIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            9071..10474
                     /gene="baeS"
                     /locus_tag="J1Z28_003370"
     CDS             9071..10474
                     /gene="baeS"
                     /locus_tag="J1Z28_003370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBA7718175.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            10471..11193
                     /gene="baeR"
                     /locus_tag="J1Z28_003371"
     CDS             10471..11193
                     /gene="baeR"
                     /locus_tag="J1Z28_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBA7718176.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            11373..11705
                     /locus_tag="J1Z28_003372"
     CDS             11373..11705
                     /locus_tag="J1Z28_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBA7718177.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         2556 a         3196 c         3255 g         2726 t
ORIGIN      
        1 tcggcttatc tcctttcgcc atgcaagcag tctatcgcta acgcgtagat aaaatagttt
       61 catgtgttat tactggatgc gtgctcgcaa atgtgcccgt cattcagacg attcccgaca
      121 gtgtttcata attcctccat ttttctccct tattggctgg ctacactagt atcattccgc
      181 gaaacgtttc aggaagagaa actcttaacg atgaaaggca gttataaatc ccgttgggta
      241 atcgtaatcg tggtggttat cgccgccatc gccgcattct ggttctggca aggccgcaat
      301 gactcccaga gtgcagcccc aggggcgacg aaacaagcgc agcaatcgcc agcgggtggt
      361 cgccgtggta tgcgtgccgg cccattagct ccggttcagg cggcgaccgc agtagaacag
      421 gctgttccgc gttacctcac cgggcttggc accattaccg ccgctaatac tgttacggtg
      481 cgcagccgcg tggacggtca actgatggcg ttacatttcc aggaaggcca gcaggtcaaa
      541 gcaggcgatt tactggcaga aattgaccca agccagttca aagttgcatt agcacaagcc
      601 cagggccaac tggcaaaaga taaagccacg cttgccaacg cccgccgtga cctggcgcgt
      661 tatcaacaac tggcaaaaac caatctcgta tctcgtcagg aactggatgc ccaacaggcg
      721 ctggtcagtg aaaccgaagg caccattaag gctgatgaag caagcgtcgc cagcgcgcaa
      781 ttgcaactcg actggagtcg cattaccgca ccagtcgatg gtcgcgttgg tcttaagcaa
      841 gttgatgttg gtaaccaaat ctccagtggc gacaccaccg ggattgtggt gatcacccaa
      901 acacatccta tcgatctggt ctttactttg ccggaaagcg atatcgccac cgttgtacag
      961 gcacaaaaag ccggaaaacc gctggtggta gaagcctggg atcgcaccaa ctcgaagaag
     1021 ttaagtgaag gcacgctgtt aagccttgat aaccaaatcg atgccactac cggtacgatt
     1081 aaagtgaaag ctcgctttaa taatcaggat gatgcgcttt tccctaatca gtttgttaac
     1141 gctcgcatgt tagtcgatac cgaacaaaac gccgtggtga tccccaccgc cgcactgcaa
     1201 atgggcaacg aaggccattt tgtctgggta ctgaatagcg aaaacaaagt cagcaaacat
     1261 ctggtgacac cgggcattca ggacagtcag aaagtggtga tccgtgcagg tatttctgcg
     1321 ggcgaccgcg tagtgacgga cggcattgat cgcctgaccg aaggggcgaa agtggaagtg
     1381 gtggaagccc agagcgccac cgcaccggaa gagaaagcca ccagccgcga atacgcgaaa
     1441 aaaggagcgc gctcctgatg caggtgttac ccccgagcag cacaggcggc ccgtcgcgcc
     1501 tgtttattat gcgtcctgtc gccaccacgc tgctgatggt ggcgatctta ctcgccggga
     1561 ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga agtggactat ccaaccattc
     1621 aggtggtcac gctttatcca ggtgcgagcc cggatgtcat gacctctgcc gttaccgcgc
     1681 ctctcgaacg ccagttcggg cagatgtctg gcctgaaaca gatgtcgtcg caaagttccg
     1741 gcggtgcgtc agttatcact ttacagttcc agctaacatt gccgctggat gtcgccgagc
     1801 aggaagtgca ggccgcgatt aacgctgcga ccaacctgct accgagcgat ctgcctaacc
     1861 cgccggttta cagcaaagtg aacccggcag atccaccgat catgacgctc gccgtcacct
     1921 caaccgccat gccgatgacc caggtggaag atatggtgga aacccgcgtc gcgcagaaaa
     1981 tctcgcagat ttccggcgta gggctggtca cactttccgg cggccagcgt ccggctgttc
     2041 gcgtcaaact taacgctcag gcgattgccg ccctcggcct gaccagcgaa accgtacgca
     2101 ccgctattac cggcgctaac gttaactcgg caaaaggtag cctcgacggc ccttcccgcg
     2161 cggtcacgct ttccgctaac gaccagatgc aatccgccga agagtatcgc cagctaatca
     2221 ttgcctacca gaacggcgca ccaattcgtc tgggcgatgt cgctactgta gagcaaggag
     2281 cagaaaacag ctggctcggc gcatgggcta acaaagaaca ggccattgtg atgaatgtcc
     2341 agcgccagcc cggtgctaac attatctcca ccgccgacag catccggcag atgctaccac
     2401 agctcaccga gagtctgccg aaatcggtga aggtgacggt actttctgac cgtaccacca
     2461 atatccgcgc atcggtcgac gatacccagt ttgagctgat gatggctatc gcgctggtag
     2521 tcatgattat ctacctgttt ttgcgcaata ttccggcgac catcattccc ggtgttgctg
     2581 taccgctgtc gttaattggc acctttgcgg tcatggtgtt tctcgatttt tcaatcaata
     2641 acctgacgtt gatggcgtta actatcgcca ccggattcgt ggtcgatgac gccatcgtgg
     2701 tgatcgaaaa tatttcccgc tatatcgaaa aaggcgaaaa accgttggcg gcggcgctca
     2761 aaggcgcggg tgaaatcggc tttaccatta tctcgctgac cttctcgctg attgcggtgt
     2821 tgatccccct gctgtttatg ggcgatatcg tcgggcgact gttccgcgaa tttgctatta
     2881 ccctggcagt cgcgattttg atctcggcgg tggtgtcgct gaccctgaca ccgatgatgt
     2941 gcgcgcggat gctcagccag gagtcgctgc gtaaacagaa ccgcttctcc cgtgcctcgg
     3001 agaaaatgtt cgacaggata atcgccgcct atggtcgtgg actggcgaaa gtgctgaatc
     3061 atccgtggct gaccttaagc gtggcgctca gcacgctgct gcttagcgtg ctgctgtggg
     3121 tgttcattcc gaaaggtttc ttcccggtgc aggacaacgg cattattcag ggcactttgc
     3181 aggcaccgca atccagctcc tttgccaata tggcccagcg gcaacgccag gtcgcggacg
     3241 tgattttgca ggatccggca gtgcaaagtt tgacctcatt tgttggcgtt gatggcacta
     3301 acccgtcgct gaacagtgcg cgtttacaga tcaatctcaa accactggat gaacgagatg
     3361 accgggtgca aaaagtcatc gcccgtctgc aaacggcggt ggataaagtg ccgggcgtcg
     3421 atctcttcct gcaaccaacg caggacctga ctattgatac tcaggtcagc cgcacccagt
     3481 accagtttac cttgcaggcc acgtcactgg atgcgctcag tacctgggtg ccacagttga
     3541 tggaaaaact ccagcagctg ccacagcttt ctgatgtctc cagcgactgg caggacaaag
     3601 ggctggtggc gtatgtcaat gtcgacaggg acagcgccag ccgtctgggg atcagcatgg
     3661 cggatgtcga taacgccctg tacaacgcgt ttggtcagcg actgatttcc actatttata
     3721 ctcaggctaa ccagtatcgc gtggtgctgg aacacaacac cgaaaatacc ccaggcctcg
     3781 cggcgctgga taccattcgc ctgaccagca gcgacggtgg cgtggtgccg ttaagctcaa
     3841 ttgccaaaat tgagcagcgt tttgcgccgc tttccatcaa ccatctggat cagttcccgg
     3901 taacgactat ctcctttaac gtgccggata actattcgct gggcgatgcg gtgcaggcga
     3961 ttatggacac agagaagacg ctgaatctgc cggtggatat caccacgcag ttccagggca
     4021 gcaccctcgc cttccagtcg gcattgggca gcactgtctg gctgattgtc gcggcggtgg
     4081 tggcgatgta tatcgtgctc ggcattctgt acgagagctt tattcacccg atcaccattc
     4141 tctcgacgct acccaccgca ggggtcggcg cgctgctggc gttaatgatt gccgatagcg
     4201 aactggatgt gattgccatt atcggcatta ttttgctgat cggtatcgtg aagaagaacg
     4261 ccatcatgat gatcgacttc gcgctggctg ctgagcgcga gcaaggcatg tcgccgcgcg
     4321 aagctattta ccaggcttgt ctgttgcgtt ttcgtccgat cctgatgacc actctggcgg
     4381 ctctgcttgg tgcgctgccg ctgatgttga gtaccggggt cggcgcggaa ctgcgtcgtc
     4441 cgttaggtat cggcatggtc ggcggtctga ttgtcagcca ggtgctgacg ctgtttacca
     4501 cgccggtgat ttatctgctg ttcgaccgtc tggcattgtg gaccaaaagc cgctttgccc
     4561 gtcatgaaga ggaggcgtaa gtgaagtttt ttgccctctt catttaccgc ccggtggcga
     4621 cgattttact gtcggtcgcc attaccctgt gcggcatact gggtttccgt atgctgccag
     4681 tcgccccgct gccgcaggtc gattttccgg tgatcatggt cagcgcctcg ctgcccggtg
     4741 cgtcaccaga aacaatggcg tcttccgttg ccacgccgct ggagcgctca cttgggcgca
     4801 ttgccggagt cagcgaaatg acctccagca gttcgctcgg cagcacgcgc attattttgc
     4861 agtttgattt tgaccgggat atcaacggcg cagcgcgcga tgtgcaggcg gcgatcaacg
     4921 ccgcgcaaag tctgctaccc agcgggatgc ccagccgtcc gacctatcgc aaagcgaacc
     4981 cgtcggatgc gccaattatg atcctcacgc tgacatccga tacttattcg cagggtgaac
     5041 tgtacgattt cgcctcgaca cagctggctc cgacgatttc gcaaatcgac ggtgtcggtg
     5101 atgtcgatgt tggcggcagc tcgctgcccg ccgtacgcgt cgggctgaat ccgcaggcgc
     5161 tgtttaatca gggcgtctcg ctggacgacg tacgcaccgc catcagcaat gccaacgtgc
     5221 gtaaaccgca gggggcgctg gaagatggta ctcaccgctg gcagatccag actaacgatg
     5281 aactgaaaac cgccgctgaa tatcagccgc tgattattca ttacaacaac ggcggcgcgg
     5341 ttcgtctggg cgatgtggcg acggtgaccg actctgtaca aaatgtacgt aacgccggga
     5401 tgaccaacgc caaaccggct attttgctga tgatccgcaa gctgccggaa gccaatatta
     5461 tccagacggt agacagcatc cgcgcccgtc tgccggaatt acaatccacc atcccggcag
     5521 caattgatct gcaaattgcc caggatcgct cccctactat ccgcgcgtcg ctggaagagg
     5581 ttgagcagac gctggtaatc tcagttgcac tggtgattct ggtggtgttt ttgttcctgc
     5641 gttcgggtcg cgccaccatt attcccgccg ttgcggtgcc ggtttcgctg attggtacgt
     5701 ttgcggcgat gtacctgtgt ggtttcagtc tcaataatct ttcgttaatg gcgctcacca
     5761 tcgccactgg ctttgtggtg gatgacgcca tcgtggtgct ggaaaacatt gcccgtcatc
     5821 tggaggcggg gatgaaaccg ctacaggccg cgctgcaagg tactcgtgaa gtcggcttta
     5881 cagtgctgtc gatgagtctg tcactggtgg cggtgttcct gccgctgctg ttgatgggcg
     5941 gattgccggg tcgcctgtta cgcgagtttg ccgtgacgct ttctgtcgcc attggtattt
     6001 cgctgctggt ttccctgacg ttaacgccga tgatgtgcgg ctggatgcta aaagccagca
     6061 agccgcgcga gcaaaagcga ctgcgtggct ttggtcgcat gttggtagcc ctgcaacaag
     6121 gctatggcaa gtcgctgaaa tgggtgctca atcatacccg tctggtgggc gtggtgctgc
     6181 ttggcaccat tgcgctgaat atctggctgt atatctcgat cccgaaaacc ttcttcccgg
     6241 agcaggacac cggcgtactg atgggcggga tacaggctga ccagagtatt tcgtttcagg
     6301 cgatgcgcgg taagttgcag gacttcatga aaattatccg tgacgatccg gcggtggata
     6361 atgtcaccgg ctttaccggc ggttcacggg tgaacagtgg aatgatgttt atcaccctta
     6421 aaccacgcgg cgagcggagc gaaacggccc aacagattat cgatcgcttg cgtaaaaaac
     6481 tggcgaaaga accgggagcg aatctgttcc tgatggcggt acaggatatt cgcgttggcg
     6541 gacgtcaggc taacgccagt taccagtaca cgttgttatc cgacgacctg gcggcactgc
     6601 gcgaatggga gccgaaaatc cgcaaaaaac tggcgacatt gccagagctg gctgatgtta
     6661 actccgatca ggaagataat ggcgcggaga tgaaccttat ttacgatcgc gacactatgg
     6721 cgcgattggg aattgacgtg caagccgcca acagcctgtt aaataacgcc ttcggtcagc
     6781 ggcaaatctc gaccatttac cagccgatga accagtacaa agtggtgatg gaagtggatc
     6841 cgcgctatac ccaggacatc agtgcactgg aaaaaatgtt cgttatcaat aacgaaggaa
     6901 aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc gaacgctcca ctgtcggtga
     6961 atcatcaggg attatcggcg gcctcgacca tttcgttcaa cctgccgacc gggaaatcgc
     7021 tctcggacgc cagtgcggct atcgatcgcg caatgaccca gcttggtgtg ccttcgacgg
     7081 tgcgcggcag ttttgccgga acagcgcaag tgttccagga gacgatgaac tcacaggtga
     7141 tcctgattat cgccgccatc gccacggtat atatcgtact gggtatcctt tacgagagtt
     7201 atgtgcatcc actgacgatt ctctccaccc taccctcggc gggcgtcgga gcgctgttgg
     7261 cgctggagct gttcaatgcc ccgttcagcc taattgcgct gatagggatc atgttattaa
     7321 tcggcatcgt gaagaaaaac gccattatga tggtcgattt tgcgcttgaa gcccaacggc
     7381 acggtaacct gacgccgcag gaagctattt tccaggcctg tctgctgcgt tttcgcccga
     7441 ttatgatgac taccctggcg gcgctgtttg gtgcgctacc gctggtattg tcgggcggtg
     7501 acggctcgga gttgcgccaa ccattgggga tcaccattgt cggcggtctg gtcatgagcc
     7561 agcttctgac gctgtatacc acgccggtgg tgtatctctt tttcgaccgt ctgcggctgc
     7621 gtttttcgcg taaacctaaa caagcggtaa ccgagtaaat gacagatctt cccgacagca
     7681 cccgctggca attgtggatt gtggctttcg gcttctttat gcagtcgctg gacaccacca
     7741 tcgttaacac cgcgcttccc tcaatggcgc aaagcctcgg ggaaagtccg ttgcatatgc
     7801 acatggtcat tgtctcttat gtactgaccg tggcggtgat gctgcccgcc agcggctggc
     7861 tggcggacaa agtcggcgtg cgcaatattt tctttaccgc catcgtgctg tttactctcg
     7921 gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact gttgctggca cgcgcgctac
     7981 agggcgttgg cggcgcgatg atggtgccgg tcggcaggtt gacggtgatg aaaatcgtac
     8041 cacgcgagca atatatggcg gcgatgacct ttgtcacgct gcccggtcag gtcgggccgc
     8101 tgcttggccc ggcgctcggc ggtctgctgg tggagtacgc atcgtggcac tggatctttt
     8161 tgatcaacat tccggtgggg attattggcg cgatcaccac actgatgtta atgccgaact
     8221 acaccatgca gacgcggcgc tttgatctct ccggctttct gttgctggcg gttggcatgg
     8281 cggtattaac cctggcgctg gacggcagta aaggtacagg tttctcgccg ctggcgattg
     8341 ctggcctggt cgcagttggc gtggtagcac tggtgcttta tctgctgcac gcccaaaata
     8401 acaaccgcgc cctgttcagt ctgaaactgt tccgcactcg taccttttcg ctgggcctgg
     8461 cggggagctt tgccggacgt attggcagtg gcatgttgcc ctttatgaca ccggttttcc
     8521 tgcaaattgg cctcggtttc tcgccgtttc atgccggact gatgatgatc ccgatggtac
     8581 tcggcagcat gggaatgaag cgaattgtgg tacaggtagt gaatcgcttt ggttatcgtc
     8641 gggtactggt ggcgaccacg ctgggcctgt cgctggtcac cctgttgttt atgactaccg
     8701 ctctgctggg ctggtactac gttttgccgt tcgtcctgtt tttacaagga atagtcaact
     8761 cgacgcgttt ctcctccatg aacaccctga cgctgaaaga tctcccggac aacctggcga
     8821 gcagcggcaa cagcctgctg tcaatgatta tgcaattgtc gatgagtatt ggcgtcacca
     8881 tcgccgggct gttgctggga ctttttggtt cgcagcatgt cagcgtcgac agcggcacca
     8941 cacaaaccgt ctttatgtac acctggctta gcatggcgtc tatcatcgcc cttccggcgt
     9001 tcatctttgc cagagtgccg aacgatacgc atcaaaatgt ggctatttca cggcgaaaaa
     9061 ggagcgcgca atgaagttct ggcgacccgg tattaccggc aaactgtttc tggcgatttt
     9121 cgccacctgc attgtcttat tgatcacgat gcactgggcg gtacgtatca gttttgagcg
     9181 cggctttatc gattacatca agcatggtaa tgaacaacgg ctgcaaatgc tcggcgatgc
     9241 gcttggtgag cagtacgccc agcacgggaa ctggcgcttc ctgcgtaata acgatcgctt
     9301 tgtatttcag atcctacgtt cactggagca tgataacaac gaagataagc ccggccccgg
     9361 tatgccacca cacggctggc gcacgcaatt ttgggtggtt gatcaaaata ataaagtgct
     9421 ggttggcccg cgagcaccgg ttccacccga cggcacacgg cggcccatta tggtcaatgg
     9481 tgcggaagtt ggcgcggtga tcgcctcccc tgttgaacga ctgacccgta atactgatat
     9541 caattttgac agacaacagc ggcaaaccag ttggctgatt gtggctttat ctaccttatt
     9601 agcggcgctg gcgacattcc cactggcgcg cggtttgctg gctccggtaa aacgactggt
     9661 ggatggcacg cacaaactgg cagcgggtga tttcactact cgcgtagccc ccaccagcga
     9721 agatgagttg ggcagactgg cagaggattt caatcaactc gccagcacac tggagaaaaa
     9781 ccagcagatg cgccgcgatt ttatggccga tatttctcat gaactgcgca cgcctttagc
     9841 agtactgcgc ggcgaactgg aagccattca ggatggtgtg cgtaaattca cgccggagac
     9901 gctggcttct ttacaggcag aagtcggaac actgacaaaa ctggttgacg atctccatca
     9961 attgtctatg tctgatgaag gcgctctcgc ctatcaaaaa gcaccggtag atttgatccc
    10021 actgctggaa gtggcgggag gcgcatttcg cgaacgattc gccagccgtg gcctgaaatt
    10081 gcaattttcc ctgccagaca gtattaccgt atttggcgat cgcgatcgtt taatgcagtt
    10141 gttcaataac ttactggaaa acagcctgcg ctacactgac agcggcggca gcctgaaaat
    10201 ctctgccgag cagcacgaca aaacggtgcg cctgaccttt gccgacagtg cgccgggtgt
    10261 cagtgacgat cagttacaaa aattgtttga acgtttttat cgcaccgaag gttcccgcaa
    10321 ccgcgccagc ggtggttccg ggctggggct ggcgatttgc ctgaacattg ttgaagcaca
    10381 taacggccgc attattgccg cccattcgcc ttttggcggg cttagcatta cagtagagtt
    10441 accgctggaa cgggatttac agagagaagt atgaccgagt taccaatcga cgaaaacaca
    10501 ccgcgtattt tgatcgtgga agatgaaccg aagctggggc agttgctcat tgattatctg
    10561 cgtgctgcga gctatgcgcc aacgttgatt agccacggcg atcaggtact gccatatgtg
    10621 cgccagacac caccggatct gatcctgttg gatctgatgc tccccggcac cgatggcctg
    10681 acgctgtgcc gggaaattcg tcgtttttct gacattccga tcgtgatggt gacggcaaaa
    10741 atcgaagaga tcgaccgcct gctggggttg gagattggcg cagatgacta tatctgcaag
    10801 ccgtacagcc cacgggaagt ggtagcgcgc gtcaaaacca ttttgcgccg ttgcaaaccg
    10861 cagcgtgagt tgcagcaaca ggatgctgaa agcccgttga ttatcgacga aggtcgtttt
    10921 caggcttcct ggcgcggtaa aatgcttgac ctgacgcctg cagaatttcg tctgctgaaa
    10981 acgctctctc atgaaccagg aaaagtgttc tcccgcgagc agttgctgaa ccatctttat
    11041 gacgactacc gcgtggtaac cgaccgtacc atcgacagcc acattaaaaa cctgcgccgc
    11101 aagctggaat ctctcgacgc cgaacagtca tttatccgcg ccgtttacgg cgtcggttac
    11161 cgctgggaag ccgacgcctg ccgcatcgtt taacattttt acctgccgga ggtggccttc
    11221 agaccagatc cggcaacatt atcccacgca tggtcagcaa actgacatta tctctccgcc
    11281 ctggcaggca ccagaaaatc gctcatactt taattggcaa acagtacctt tagatgctgt
    11341 tttcgatacg caatttcaat caaggagtca ttatggctgg ttggtttgaa ctcagtaaga
    11401 gcagtgataa tcagttccgg tttgtgctaa aagcgggcaa tggtgagact atcctcacca
    11461 gcgagctgta tacctcaaaa gcctctgcgg aaaagggcat cgcgtcggtg cgtagcaaca
    11521 gcccgcaaga agaacgctat gagaaaaaaa cggcaagcaa tggcaaattc tatttcaatc
    11581 tgaaagccgc caatcatcaa attatcggct ccagccagat gtatgccacc gcgcaatctc
    11641 gtgaaaccgg aatagcctcc gttaaagcaa atggcacaag ccagacggtg aaagacaata
    11701 cgtaattacg atgccgggcg taggttgttg gcg
//
LOCUS       DADSZD010000135        11627 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.136, whole genome shotgun sequence.
ACCESSION   DADSZD010000135 DADSZD010000000
VERSION     DADSZD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.136"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            165..815
                     /gene="zinT"
                     /locus_tag="J1Z28_003373"
     CDS             165..815
                     /gene="zinT"
                     /locus_tag="J1Z28_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBA7718178.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(1485..1574)
                     /locus_tag="J1Z28_003374"
     tRNA            complement(1485..1574)
                     /locus_tag="J1Z28_003374"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1538..1540),aa:Ser,seq:cga)
     gene            1668..2465
                     /gene="mtfA"
                     /locus_tag="J1Z28_003375"
     CDS             1668..2465
                     /gene="mtfA"
                     /locus_tag="J1Z28_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBA7718179.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEILIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2566..2641
                     /locus_tag="J1Z28_003376"
     tRNA            2566..2641
                     /locus_tag="J1Z28_003376"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2599..2601,aa:Asn,seq:gtt)
     gene            2803..4065
                     /locus_tag="J1Z28_003377"
     CDS             2803..4065
                     /locus_tag="J1Z28_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7718180.1"
                     /translation="MSLTSAKIRTLKPSDKPFKVSDSHGLYLRVKPGSSRHWYLKYRI
                     SGKESRIALGTYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4259..5563)
                     /gene="ybtS"
                     /locus_tag="J1Z28_003378"
     CDS             complement(4259..5563)
                     /gene="ybtS"
                     /locus_tag="J1Z28_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     YbtS"
                     /protein_id="HBA7718181.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGETYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5591..6871)
                     /gene="ybtX"
                     /locus_tag="J1Z28_003379"
     CDS             complement(5591..6871)
                     /gene="ybtX"
                     /locus_tag="J1Z28_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HBA7718182.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVSQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6864..8666)
                     /gene="ybtQ"
                     /locus_tag="J1Z28_003380"
     CDS             complement(6864..8666)
                     /gene="ybtQ"
                     /locus_tag="J1Z28_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HBA7718183.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGAASGEWVHE"
     gene            complement(8653..10365)
                     /gene="ybtP"
                     /locus_tag="J1Z28_003381"
     CDS             complement(8653..10365)
                     /gene="ybtP"
                     /locus_tag="J1Z28_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HBA7718184.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVME
                     NERQ"
     gene            10622..11581
                     /gene="ybtA"
                     /locus_tag="J1Z28_003382"
     CDS             10622..11581
                     /gene="ybtA"
                     /locus_tag="J1Z28_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HBA7718185.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQHRCNAETHLTA
                     QALRHALMRMHSGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
BASE COUNT         2914 a         3248 c         2871 g         2594 t
ORIGIN      
        1 ttgtcatcaa cttttcatca ccagaataat ttattttatt tcaataagat agttcaatac
       61 acctaagaat accatgctct catttcctaa tgagttgttg cattttgcca catgttacaa
      121 tataacatta cacatcatgt acattaactc tggaggaaat tgttttggcg attcgtcttc
      181 ataaactggc tgttgcttta ggtgtcttta ttgttagcgc tcctgccttt tcgcatggtc
      241 atcattctca tggcaaaccc ttaacagagg tcgaacaaaa agctgctaat ggtgtttttg
      301 atgataccaa tgtacaaaac cgaacgctca gtgactggga tggagtctgg caatccgttt
      361 accctttact gcaaagtggc aaacttgatc ccgtctttca gaagaaagcg gatgcagata
      421 aaaccaaaac atttgctgaa attaaagatt attatcgcaa aggttatgcg acagatatcg
      481 aaatgattgg cattgaggac ggcattgtcg aattccatag aaataatgaa acaacatcct
      541 gtaaatatga ttacgatgga tacaaaatac tcacctataa atcaggcaag aaaggcgttc
      601 gttacttatt cgaatgtaaa gatcctgaaa gcaaagcccc taaatatata caatttagcg
      661 atcatataat tgcaccacga aaatcatctc attttcacat ttttatgggt aatgattccc
      721 aacagtcttt attgaatgaa atggaaaact ggccaacgta ttatccctat cagttgagta
      781 gcgaagaagt ggtcgaggag atgatgtctc attgattttt ttgcgctgac ttcagttatt
      841 taacagaaca gtgcctggta gtaacaacaa taatttacca agacaaataa gtaagacaaa
      901 ctcagacacg ctcatacttg agtcgtgtct tttttattat gtctgtagaa aaaaactgag
      961 aaaaaggcga acaataaata cacaactcag ccatacagac tggcagcgca aatgtttcca
     1021 ggagcaagtc atctgtcatg gtaaagctaa ccagaaatat aggttaattt ttgtgtttaa
     1081 acctttttta cttgttagta tgatacaaag gctttcaaaa aagctgggaa attcaacaac
     1141 tcttggaaat tatggacttt gtcccgcggc tttaatctga cggccgcgtt ccttttttgg
     1201 ctcaattatt gaccccttct ctacgcaact tcagttcccg ccagcaactt tgcggcagtt
     1261 tcataggatc aatgtctaaa agaataatgg tgaccgataa gaaaacgact gaataactgt
     1321 agattttcgc tcgaaacctt cctgtcagct ccatagcgaa tcaagtgctg aatgtcacag
     1381 tatcgaacag aaaacagtga cgatctaacc cttcaagaat attctacgat tgttctgttt
     1441 aggaaaagca aggcgggaag tcgggagata agtcattgat aaagtggcgg agagaggggg
     1501 atttgaaccc ccggtagagt tgcccctact ccggttttcg agaccggtcc gttcagccgc
     1561 tccggcatct ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt
     1621 ccctgtccgt gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc
     1681 cctggaaagt acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga
     1741 tccccctttt aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac
     1801 gttttttaca gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa
     1861 gctgccggat agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg
     1921 atggttttca tgaaatctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag
     1981 acgatatcgg tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag
     2041 ggcctatcgt tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc
     2101 tgattattca tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag
     2161 ttccctttat ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga
     2221 acaacattca ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg
     2281 ctgccagtga tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag
     2341 aactttttgc tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag
     2401 atcctttgca gagactgcat cacgctaatg atacagactc gttttcggcg acgaatgttc
     2461 attaattaac aactttgcag attaattaac caattgaaat ggcttatgaa atttagtgtt
     2521 gacagacaag gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag
     2581 tcggtagaac ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc
     2641 aattttctgt tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt
     2701 gtgaaaactc ttctcagtgc attttgattt taccctacgc atcgggaaaa attggtggtc
     2761 aaatctgggg tcaggttagt tcgataatgg agtgaccccc atatgtccct taccagcgca
     2821 aaaatccgca ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg
     2881 tatctgcggg tcaagccggg tagctcccgc cactggtatc tcaaataccg tattagcggt
     2941 aaagaatccc gcattgcgct gggtacctat ccagccatct ccctgtctga tgcgcgacag
     3001 caacgtgaag gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct
     3061 gctgtacgtg gctcacgaac accggagaaa gtttttaaaa acgtggcgct ggcgtggcat
     3121 aaaagtaaca ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag tctgaacaat
     3181 cacatctttc cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt
     3241 gacctgctga aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag
     3301 cacctgagta acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca
     3361 gcaaaccttg gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg
     3421 gagcggctgc ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc
     3481 cggcatgccg ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc
     3541 cgctggtcag agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc
     3601 attattggcg tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc
     3661 ctctcagaac agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa
     3721 ctgatcttcc ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag
     3781 gcactgcggg tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca
     3841 atggcatgca gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag
     3901 atgagtcatc aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg
     3961 gaagcccgca aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct
     4021 tatgcaccgc cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc
     4081 cacactctgg caaattacca ccacctccac aggaggtggt tctatataga cttttatcaa
     4141 cttcatgacg aaaaacacct attttatccc aaaagaagac aatagagtca cagaggaatc
     4201 aaagagcgac tcaattgtct ttggacaatc tggttgtgag gttagaaaac agttactcct
     4261 acaccattaa atagggcgca atgctcgcta atttctcccg ggtttctgtc agttcgcgtt
     4321 ccggcgtaga ttgcgcgatg attcccgccc ccgcctgtat ccagcagcgc tggctatcct
     4381 gaaatacgga acgcagaact agcgccgcat caaagcgcgt atcgtccagc agcaggattg
     4441 cgccggaata aagctctcgc ggcgtcttct caatttgcat aatcgcgttc agagcagcat
     4501 ttttagggat acctgaggcg gtaatcgacg gaaacagcac ggtaaacgca tcccaggcat
     4561 ccttgttttc cgcaagctga ccgctcacgc cagaccccag atgctgaacg ctgccgcgct
     4621 ggcgaaccga cattaaatct tcaaccacca cgctgcccgg ctggcaaacg gcctccagtt
     4681 cagcaatagc ttctttgaca gaaaggatat gctcaagcac ctctttactg tcgtgcagca
     4741 gttctgcctc tttcgcctta ttatgctccg ggtttcccat gcgatcgcgg gtgcccgcaa
     4801 gcggttcagt gaccacttta ttgcccgtca ctgacatcac cagttccggg ctaaagccca
     4861 gcgcttcgcg tccttcctga cggaacataa acgagcgcac aggcgtgttt gcctgccgcc
     4921 cgtataacag cgtggcgggc atatcaatcc gcgatggcag gggaatggcg cgcgagacaa
     4981 tcactttgac atactcgcca cggcggatct ccgcaacggc gcgcgcaacc tgttgtttat
     5041 atgtctcacc attgagggcg gtatccaccg ccagtggtgc gttctgcgtc gttgtaccgg
     5101 cctcttttac ccactcgcac aaacgtcggc acccgtcggc ggagtccgca taaacggtga
     5161 catttccctt ttcaaaaata agttcttcac ggggaacggt taacgtcagc agcggccact
     5221 ccccggcgtt aaaggcaatc ccccgcgcat gcgccgcaaa gttgaaccca acctgtccat
     5281 atacccgacg tcctttcacc tgaggatgcg ccataaaacg acgcgcgcag tcggcaatgg
     5341 aatccaccgc ccatttttgc tccccggcat catcaataaa tgtcgcctgc gttccgtcgg
     5401 cattaatgtg cagccgtgcc tggcaccctt tgcctaaata ccaacagggt tgacgctcat
     5461 agacataaca ctcttcttct gcgaactggc gtaaaacgcc agaaatcgtc ggtagccatt
     5521 gttcctctgg taacgccaga tgtagaaatt cactgatttt catcccttac ctctctgtgt
     5581 tattcgcgga tcaatcaatt gcgcttgtca tcagttctct tgccgaatgc agccggatat
     5641 aagcaaccca cgccgccagc aacgtgaatg ccccggcaaa caggaagcag gccgcatagc
     5701 caaaatgttg agcaacaacg ccaccgataa cgcctgccag aatggcgatt gccgcatcag
     5761 tacattgaaa gagggtgaag tcgacaccgg cctgcaaagg cgatgaaagc gacatcagcg
     5821 tggcgtaaag acagaccagc gcgcaggcca gcgaaatgga ctggacaatg accagacact
     5881 ggagaatctg cagcagcaga tgacccggtg ccatcatgag cgtcatcacc accgccagca
     5941 gcgcgatccc ctggacgcca taagccgtca acagcgctct gccgggtgag gtgtatttca
     6001 tcagcaatcc gccggccagc gttcctgcta tgcccgctgc aatattgccg ccgctgaaca
     6061 gcgcgcccaa tgcggacatg ctcaacccat gatccaacaa cagcggtgcc agaagaggca
     6121 gcacaaaccg catgcctgaa ttcagcatca ataccagcag taagcccagg cgcgcctgct
     6181 tcctccttag cgcataacct aacgccggaa catgcgggat agtcgctgtt cgcgtcggct
     6241 ccgtaatgcg ccacagcggg agtgacagcg ccataatcag caccgccatc atcagcatgg
     6301 cgacaggcca gccggatgcc gccgacagca tcaggaacac cccgccgccg cacatcattc
     6361 ccagatagct gccgccgacc tgcacgctgt ttccccagcc gtaacccgtg cgggtcagtt
     6421 gatcgacaca aaagccgtcg ctggcgatat caatggtgga ggcgaccgtg cctgccacca
     6481 taaacagaac aaataacgcg acgatttgcg tatcggggaa tcccccttct cgcccaaacc
     6541 agccaatcgc tgcggcaata gtcaggatcg ccgctagcgc gacctgacca cgcaggatta
     6601 acatgcggga acggcgttcc tggctaccgg gcggaagccg ccagcgctcg atccacggcg
     6661 cccaaataaa cttcagcgcc cagggcagca taaacagggt tgtcgcaccg gcaagcgcca
     6721 gcgaaccgcc agcagcgcgc accagcgcgg gtaaggactg catagacagc gccgatacca
     6781 gactctgcga cacatagacg ccggcaatag caaaaatcac ccgcccggtc gtcaacgtca
     6841 gaggtttcac attcgactga acatcactca tgcacccact ctccagacgc ggcccccccg
     6901 tcgtcgcgcc acacgcgcgc ggccatttgc gcctgccaca gtgcctgata acgtccatga
     6961 tgtgagagca attgcgcatg agtaccctgc tcaaccacct gtccctcttc catcaccaga
     7021 atgtttccgg ccccggcgat ggtggataaa cggtgagcga tgataatcac cgtccggttg
     7081 tgaaccaggt tatcgatcgc tttttgcacc gccagctcgc tttcaatatc cagcgcggca
     7141 gtcggttcat cgagaatgac gaccggcgcg tttttcaata acgctctggc aatggaaatc
     7201 cgctggcgct cgccgcccga tagctggccg cccatctctc ccattggcgt cagccagcct
     7261 tgcggaagac gggaaataaa ctcaaggcac tgcgccgcgc gggcggcctc ttctacctcc
     7321 tgccgcgtcg cctgtgggcg cgcgatacgg atattcgcca gcagcgtgtc atcaaacagc
     7381 cagacatcct ggaaaacaac agaaatcagg ctattgagct gttccggcgt caggcggcga
     7441 atatcgacgc cgccaataga aatctgccct tgctgcggat cggcatagcg cattaacagt
     7501 ttggtgacgg tagttttgcc tgcgccggag gcacccacca gcgcgctcat actggctgcc
     7561 gggaacgtca aagaaacatg attaagcgcg tggccgtcgc cttcttcgta gcgatagctg
     7621 acgttgtcaa agcggatatc gtaacgttcc ggcatctcgc tttgctctgc gacgggaagc
     7681 ggtgctatcg ccataaactg ctcaatccgt tgcagggcgc tggcgatcag ttccacaacc
     7741 gaggtgtagc tgataaacat cgccatcggt tctgcgaagc gcataatcat cgcgacggcg
     7801 gcaatcaaaa aggcgaggtt cagagtgccc gtcactaccc agacgatccc ggataacacc
     7861 accacctgta ggcccaactc cacgacgctg gcgatcagca tcgtcgcgcc agcgccctga
     7921 cggtgagtgc gggtctgtaa gttttccagc gcattgaaat gcgccagcag cgcccggctt
     7981 ttatcggcat cgctgccgca ggtgcgcaat accatcatcc cctgagcaaa ttcaacgata
     8041 tcgccgctca gacgctggtg cgcttccccc agcgtctgca tttgtcgccg catcgccggg
     8101 cggcgccagt aataaaacgg caccagcaga gggaagatca gcaacatcac cagccccagc
     8161 cgccagtcta tccacaatgt cgccagcgac gccgtcagcg gcgtgacaat ggtgagcagc
     8221 aaaatattgg ctatcgcaat aacataattg aggttttcat ccacgctgcc cagcagcaag
     8281 gcgttcattt cacccgccct gccgcgctgg agcttctcca gcggcacgcg gcgtaactgc
     8341 tcgccaagtc gcaggcgcaa ctcatgggta gcctgcgcca gatgaccacg gtattcaaag
     8401 cccaggccat accagcgtag caccagcgtc acaattgcgg cgacgctgaa ggccatagcc
     8461 cagttaagaa gttgcggcgc gtctccccgt aacagcgcat caatgatcgg ataaagacag
     8521 gcaaaggcga tcccctgcat gaatgccgcc agcaacagcg ccagcatact gcgtcgcagc
     8581 atcctggcct gactgccaac gctggagata agctggcgcc agatgacgcg ccaggccagg
     8641 ttatccgcag gattattgtc tttcattttc catcacctct tcctgtgttc cacccggcac
     8701 ccaatgctgc gcctgctgat agtgttgcca gagccgttga tacaggccgc cctgcgccaa
     8761 cagttgcgta tggttcccca tttccctgag ctgtccgtcg gaaaacagca atatcacatc
     8821 ggcctgagtc accattgaga gacgatgcgc gaccatgatg accgtccggc cacgcatggc
     8881 cgccgcgagc gccttgataa gcgccgcttc gttttccggg tcagcaaacg ccgtcgcctc
     8941 atcgagcacc aggatggggc gatcctgcaa aagcgcccgg gcgatagtaa tgcgctgccg
     9001 ctggccgccg gagagaaata ccccacgttc cccgactcga gtgttgtaac cttctggcag
     9061 agcgctaata aaatcatggg cctgcgccac tctggccgcc gctataaccg cctccagcgg
     9121 cgtatccggc gcgcccagac gaatgttatt ggctatcgtg tcggcaaaaa ggaagttgtc
     9181 ctgaaacaca aacgagagtt gcttcatcag ggtgtccgtc tgcatatcac gcagatccac
     9241 gccgccaata cggatatggc ctttgtccgg gtcggcgtaa cgcagcagca agcgcgccac
     9301 ggtgcttttt ccggcgccgc ttggcccgac cagcgccaca atttgcccgg caggcacatg
     9361 aaagctcacc tcctgcaacg cggcgccagt acgcgcttgc ggataatgaa agctcacctg
     9421 ctcaaaggta atgctcgcct cttgcggctg ctggtcagac tgcggcagcg ataactccgg
     9481 catcgcgaga acctgataaa tacgctgaat gcttaaacgc gtttgcgcga ccaggttatt
     9541 gagcatcatc attggcatta cggcttcggc cattccgctg cccagcagta aaacggccac
     9601 ccacgcgata aaatcgaaac tgccatagtg caacaggccg tatcccgacc agatcaggac
     9661 aaacagggtc gggagaggat tcagaatcga gaaggaaaaa cgcgctgaaa aaccggcttt
     9721 gcgataccag gttttgagca catcgaccca ctcttcaagg gcacgttgat agcgcaaaaa
     9781 actggtgctg ccgctatcaa acgttctcac cacgggcatg gcctgcacga actcaataac
     9841 cgccgcgcta acctgctcgc gcgccttatg ataacgctga gccatatctt ccgaacgctg
     9901 gcgggcgagc acgagaacaa ccgatccaaa cgccagtacc cccagcgtcg cgattgccag
     9961 ccgccagtcc agccagaaca agataacgat tgtcgccagc ggcatgatga tcgcgcgggc
    10021 atagagcggc gtgctgtcgg ccacaaaaat atgtaacgac ttcacgtcat ccagcatcac
    10081 tttcgccact gacccgctgc ccatttgctg taacacgcca ggcggaagct gcaatgcttt
    10141 acgggctaac ctgctgcgca gaatgttctc cagatgaaaa gccgcgtaat gagaggtgtt
    10201 gaacgcctgt aagcgcagaa caaaagttaa gaccacgcag ccgatcacgc cgcccagcgg
    10261 ccaggccgga atagcgtcag gcgtcgcgcg aatgtcacgc aggctccacg ccagaaaaag
    10321 gagcgccccc aggctggtta atgccgccag ccccgccagc gccatagcta aaataacctt
    10381 cctgcgaacc ggtgtaatca cctgccaaag aggaaatcgg gacagcgatt cagtattgga
    10441 tgattgagat gacatgggag taactgaatt tcctgatgaa tttttacgga cgccaaaaac
    10501 gacaattcag ggtggcgttc tgagaattaa tgtgaataat aaccattatc aataactcat
    10561 ctaccccatt cggttctatt tatacccgca ttggtctaag ccacagggag ataaccaggt
    10621 catgacggag tcaccgcaaa cgcaatctga aatctctatt caccagttgg tggtcgggaa
    10681 acctgcaaac gatggcaata ttcctgctca gtgtgaactg ctgcgctgtt cgttgcagga
    10741 aggtatggat attttgctct ggaggggcca ttttgcgcgg cctgagacgt tgcagctaca
    10801 cgacgatctg ggcaggatta acttctcgtg catcctggaa ggcacatcgc gctttgcgat
    10861 tcagggactc cggcgtcaca ccgactggga gcttgcgcgc aatcgccatt acattaccca
    10921 tacgccagac tgtcgcggca gcgcctccta ttgtggccga ttcgagagca ttaccctctc
    10981 cttcagccct gaaaccctcg cgctgtgggt gccggatatt agcgcggtca ttaagaataa
    11041 gatcgactcc cactgctgtt gccagcagca ccgttgtaat gctgaaaccc acttaacggc
    11101 tcaggccttg cgtcacgcgc tgatgcgcat gcacagcggc ttcagtcatg aacaaaagcc
    11161 gtcgacgcta tggttgcagg ggcaaagtct ggtcatgctc agtctggtgc tggatgaaca
    11221 tcgcgaagac gccagttgcc tctcctgcca cttcaacccg atggaacgcc agaaactgct
    11281 gcgggcgaag gatctgctgc tggctgatct gacgcaagcg ccgggcgtcg cggagctggc
    11341 cagagagtcc ggtctgagcg ttctcaaaat taagcgaggc ttccgcgttc tgttcaacaa
    11401 cagcgtctat ggcctgtttc aggctgaacg aatgcaggaa gccagaagac gcctggccaa
    11461 cggcaatacc tccgtcatga cggtggcggc cgatctgggc tatgccaacg ccagccattt
    11521 ctctgccgcc ttccagaaac agtttggcgt aactccttcg acctttaaac gcgggatgta
    11581 accggggtcg cgccccccta aactcctccc tgacagaggc ccggata
//
LOCUS       DADSZD010000136        11598 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.137, whole genome shotgun sequence.
ACCESSION   DADSZD010000136 DADSZD010000000
VERSION     DADSZD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.137"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(134..346)
                     /gene="cspH"
                     /locus_tag="J1Z28_003383"
     CDS             complement(134..346)
                     /gene="cspH"
                     /locus_tag="J1Z28_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBA7718186.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            632..844
                     /gene="cspG"
                     /locus_tag="J1Z28_003384"
     CDS             632..844
                     /gene="cspG"
                     /locus_tag="J1Z28_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBA7718187.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            855..1043
                     /locus_tag="J1Z28_003385"
     CDS             855..1043
                     /locus_tag="J1Z28_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBA7718188.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1018..1248
                     /gene="ymcE"
                     /locus_tag="J1Z28_003386"
     CDS             1018..1248
                     /gene="ymcE"
                     /locus_tag="J1Z28_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBA7718189.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVRIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            1238..1411
                     /locus_tag="J1Z28_003387"
     CDS             1238..1411
                     /locus_tag="J1Z28_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBA7718190.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1460..2533)
                     /locus_tag="J1Z28_003388"
     CDS             complement(1460..2533)
                     /locus_tag="J1Z28_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBA7718191.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAIYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(2616..5348)
                     /gene="torS"
                     /locus_tag="J1Z28_003389"
     CDS             complement(2616..5348)
                     /gene="torS"
                     /locus_tag="J1Z28_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBA7718192.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     DFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQHQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRTQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGLVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATEIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGHIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDDL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAVQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQPLDVSQLNEDAHLMGAEKIHEWLILFKQHALPLLDEIDIARASQDNEKIK
                     RAAHQLKSSCSSLGMRSASQQCAQLEQQPLSAPLPHEEITRSVAALEAWLIRKT"
     gene            5431..6459
                     /gene="torT"
                     /locus_tag="J1Z28_003390"
     CDS             5431..6459
                     /gene="torT"
                     /locus_tag="J1Z28_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBA7718193.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6432..7124)
                     /gene="torR"
                     /locus_tag="J1Z28_003391"
     CDS             complement(6432..7124)
                     /gene="torR"
                     /locus_tag="J1Z28_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBA7718194.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7254..8426
                     /gene="torC"
                     /locus_tag="J1Z28_003392"
     CDS             7254..8426
                     /gene="torC"
                     /locus_tag="J1Z28_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBA7718195.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASAVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8426..10972
                     /gene="torA"
                     /locus_tag="J1Z28_003393"
     CDS             8426..10972
                     /gene="torA"
                     /locus_tag="J1Z28_003393"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HBA7718196.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLAVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10969..11568
                     /gene="torD"
                     /locus_tag="J1Z28_003394"
     CDS             10969..11568
                     /gene="torD"
                     /locus_tag="J1Z28_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBA7718197.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSHELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLDLLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDSFGFYAALSQLLLVLVECDYQKR"
BASE COUNT         2818 a         2914 c         3079 g         2787 t
ORIGIN      
        1 taactattag gtgcagaggt attcgcttaa atggattagt ttcctggtga aaaggtaact
       61 tgcgttataa tgtgtagtgt tgactattga actatgccaa taaaaccagt cagctttaaa
      121 caagccgctc atattaagag agataaacat ttgccgctgt tggtcctcgc aggccattta
      181 cgcggcaaaa ttccacgcgt aatcctggta taagcacttc tgcgtcgcgg ggagtgaatg
      241 cggaaatatg gacctggact tctttacgac cgtcggaggg gataatgaat cctttgccgc
      301 ttttgcgatc aaaggttttg acaattcctg tcattttacg ggacaaacaa attccttact
      361 gaaaatactg cgctgcacta tacggggtta ataaaataaa gccagcgata tttaagaccg
      421 ccggacggct aaaataaaat ttgcttaatc tcaattatca tgcgttaata gctgcgtcgg
      481 tttgaaagac agacagcata caaagtagtt tactaaagca gttctcatta tcaggcatta
      541 tccccttctt ttgagtctct ctcctgaaca ctaagtagtt tctgtattaa agccctgttt
      601 gccgaaaggc ccaaaatgaa ggaagtaaaa tatgtctaat aaaatgactg gtttagtaaa
      661 atggtttaac gcagataaag gttttggctt tatcactcct gatgatggca gcaaagacgt
      721 tttcgtccat ttcactgcca tccagagcaa tgaattccgc acgctgaacg aaaatcagaa
      781 agttgaattt tctattgagc aggggcaacg tggtcccgcg gcagcgaacg ttgttacgct
      841 ctaaggttgc cgttattact caacatctcc atttccgctg tccatgttgc catggttcac
      901 agtaccgcac atcggcattc gatgtgacgg agcgaaaccc tttgggtgca aagtgcattt
      961 tttgtaaatc gacgatgatc acctttgata acgtcgcgct gcaaatacgc actgaccatg
     1021 cgccgctgga tttcacaaaa taatatcagg ctccctcgtg gagccttttt tatatctgcc
     1081 ttatttttct tcaatgctgt acgtatagta agcgataacc tgttgattat tgaatctttc
     1141 ggggagatgg cttataacat ttcttacctg accagggtac cgggaaccaa caccttactg
     1201 gcgtgttgct gtcttttaag accagaagag gttaacagtg aatattgaag agttaaaaaa
     1261 acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa atcgccgagc tgaacaagaa
     1321 tacagggaaa gaagtctctg aaattcgctt caccgcacga gaaaaaatga ccgggcttga
     1381 aagttatgat gtcaaaatca aaataatgtg attttgtgaa catcaccccg tgcgaggtga
     1441 tgttccgctt gttgctaatt tagtgaccaa tcattggcga ttgcggaatt aagcgttggt
     1501 acaattcctc cggcaccggg ctttgccata ctcccgcata gattgcgtaa ccaatcgccg
     1561 caaacataat ccccataacc agtagcgtca ttaaccatcc agacaacgca aaggcttttt
     1621 tatttgccgc aggtttttgc agtgaaaagg tcaatgttga ggctaccgga catgactcta
     1681 cgcaagtcat acagccggta cattccactg ttcgtacctg aattaattta tcgaccggga
     1741 tccgtgatgg gcaacttttt gcgcatttgc cacaatcgat acaactttcg gcattgcgac
     1801 gaatcttaaa cggcgataat agcgaaacca cgcccatcag cgcgccatat gggcaaagat
     1861 aacgacacca ggcatggcga ataaacaggc tggcaatcag caaaacgatc atgctgatta
     1921 atgtcgcggt ccccatatga cgaaagaaat cgagcatttt aacgtccatc accacgctgt
     1981 agggcgacaa cataaaatag tgaatcgcct gagcgggcat caataacgcg atatagagaa
     2041 aaaaactcaa cagcaaatat ttcacgccgc gcagaggaat atccagccag cggggaagga
     2101 caaattgccg accaaacagt ttgttaccga gatcgccgat taattccgaa agcgtaccaa
     2161 ccgggcataa ccatgaacaa aaggcctttt tgagtaatag actgatgacg ataaaagcga
     2221 ccaataacag catcgcggcg gcgtggacgg acggtaactg acctgttgca aggctatatt
     2281 tcagattcat cagaccggca atcggtagcc agccttcgat acctcccggt ctggcgacaa
     2341 atgtcgtact acctgccgtt tcgtaatagc gcacccaata ccagaacgtg atggcaatat
     2401 aaatattaat tgccaacagt aataattgcg tcgctttacg ccaggtcgtg gcattgcgcc
     2461 agtcattcca cggtaatttg ccgcccgtcg tgcctggccg ccgctgccag cgggttcttt
     2521 ttttctctgc catgattttg ccagtccgtt aagttgcata ccaaatgcca ctattctagt
     2581 tgttcttaac tggctgatat tgattcaaat cgcgttcagg tctttcttat taaccatgct
     2641 tccagagcgg caacactgcg tgtaatttct tcgtgtggaa ggggggcaga taatggctgc
     2701 tgctccagtt gtgcgcattg ctggctggcg ctacgcattc ccagactcga gcaactgctt
     2761 tttagctgat gtgcggcacg ctttattttc tcgttatcct gactggcgcg ggcaatgtcg
     2821 atttcatcga gaagcggcag ggcatgttgt ttaaataata tcagccattc gtggatcttc
     2881 tccgccccca ttaaatgagc atcttcattg agttgcgata catccagtgg ttgatcgtta
     2941 ttgacttgca gttggagata gtgcgccagt aattgaccga gcacttcacg cggcaccggt
     3001 ttagggataa tcccgcgaaa tagcgagctg gtacgctgtc gcagtgtttc gtcaatgaca
     3061 tgggcgctaa agccaatcaa aaccagcgac ggatattgct gtgccagttg tcgggcaagc
     3121 gtaatgccgt cgatatccgg tagatcaaaa tccaccagtg cggcagcaaa cggttcacta
     3181 ttttgcagtg tttctaaagc ctgcacggca tttcctactg caacgacctg cgcaccactg
     3241 gtgttcagca tttcgacagt aattcgctgg gtgagcgggt tatcttcaat taacagcaaa
     3301 cgcaaatcgt ccagacgtac cgcctgattg actgtttttg gcacgggtgc agtggcaaca
     3361 cgtaacggca agcgtaaaca aaaacagctt ccaacctccg gcgtgctggt ggcgctcagt
     3421 tcgccgccca tcgcctgggc cagacggcta ctgatagtca gccccagccc ggtgccgccg
     3481 cgtttgccgc taacctgaac aaatggctgg aagatttctg ccagtttcgc gggatcaata
     3541 ccgcagccgc tgtcttccac ttcgaccagc cattgttcgc catcagtgcg actacgcagg
     3601 ataatgtgcc cttcgtcggt gaaacgcagg gcgttgctca acaggttggt gatgacctga
     3661 cgaatacgcc gcggatcgcc cattaacgca gtcggtacat cgtcggcaat ttctgttgcc
     3721 aggcgaatcg ggcgaccttt cacccttccg ctcattaatt gcagggtact ttccagcagc
     3781 gggcgcggtt caaagggttc atcgctgacc gaaacattct tgccgcctgc ttcgatagcc
     3841 gaataatcga gaatatcgtt gaggatggtc agcaacgatt caccagagtc ggtaatcgcc
     3901 cgcaaatcat cgcgctgggc gttaagtgcg gggttatctg ccagcagttg cgcggtgccg
     3961 agaataccgt acagcggtgt gcggatctca tggctcatcg ccgccagaaa cgccgatttt
     4021 gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt gttctatcac cagttcctgc
     4081 aattcagctg tacgcgcttt gacctgcgcc gccagctgtt cgcggtggcg attcagtgca
     4141 tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg tatccagttc ccgtacgcca
     4201 gcggtttccg ggaaagggga gtcgatatca ccatccagca agcgttgcag cgcctgcgtt
     4261 tgttctgcaa gagggcgcgt gactgagcga taaaccacgc gccagaggat cagaatcagt
     4321 gcgcaaagag aaactaatcc cagcaataac aggctgtatt gcccgcgtgc actggctttt
     4381 tccagatgtg ccagcccgtg ttgattacgc agctcaatgg tgtcgaccag ctgactgact
     4441 tcgctactaa actgcgcgaa ttgggcgatg ttattttggg cgagagtttg caggtgattg
     4501 ctgatttcac tgtcctgctg atacagcgcc agcaaatcgc tatattggct aacggtagtt
     4561 aacgttgttg ctacctgagt acgaacaccc ggatcttcaa tgcgtatttg ccgacgttgc
     4621 agaattttca ccgcattatt gagctgcttt tccagcgttg gcgcattttt ctggatctgt
     4681 tccagcccca gattcatcac catttgctgc acccgcaggg cgctaaggcg cagttcattc
     4741 atctggttaa catactcaag atcgatatca atcagccgat cgagtgcact ttctgcggcc
     4801 tgacgctgat gttgttcgat caaatcgtaa atcccggcct gggtcgctcc ggcggaggtt
     4861 gccgcattat tcgcctgacc ttgcgccagg cgtgcgatct catcggcggc ggcgactatc
     4921 tgctgactga gttgctgttg ttgctggcga agttgcaaac gctgccccac cagttcccct
     4981 tgctgacgta acgaacggga gatctcctgc tcctgttgtt caatagcggt ggtgtcaaaa
     5041 tcttgttccc gtaacgcttg cagcaacgca ttaatcttca ggctttgtgc ggtgagcatt
     5101 cgcccctgcg cctgccacat cttttcgtta tcggcactgg tcaggttctg cgcggcgaaa
     5161 agttcccagg cgctggcttc gctcaactgg cgcgccatat tcatggtagg aatcaatgcc
     5221 tgagtgttgt ctttttccac ctggctgata aagcgcaggt tgtaccatcc caccagggta
     5281 ctggtcaggg ttaacagcgc catcagggca aagcccatcc agagtcttcg ggtcagggtt
     5341 aaattcacgg tcggtgcact ttaggtgaaa aaggttgagt cgcaaagcag aatgcatcta
     5401 gcataaagcc ttattattga tgaggctatc atgcgcgtac tgctattttt acttctttcc
     5461 cttttcatgt tatcggcatt ttcggctgat aacctgttgc gctggcatga tgcgcagcat
     5521 tacacggtgc aagcctctac gccgcttaaa gccaaacgcg catggaaact gtgcgcgctt
     5581 tatcccagcc tgaaagattc atactggtta tcgttgaact atggtatgca ggaggctgct
     5641 cgccgctacg gtgtggattt aaaagtgctg gaggcaggcg gctacagcca gttggctacc
     5701 cagcaagcac aaatcgacca gtgtaaacag tggggcgcag aggccatttt gctcggtagt
     5761 agcacgacgt catttcccga tctgcaaaag caggtagcaa atctgccggt gatcgaactg
     5821 gtgaatgcta ttgatgctcc ccaggtgaaa agccgcgttg gtgtgccctg gtttcagatg
     5881 ggctatcaac cagggcgata tctggtgcaa tgggcgcacg gtaaaccact gaacgtgctg
     5941 ttgatgcccg gccccgataa cgccgggggc agtaaggaga tggtagaggg ttttcgcgca
     6001 gccattgccg gaagcccggt acgtattgtt gatattgcgc tcggtgataa cgatattgaa
     6061 atccagcgta acctgttgca ggagatgctg gaacgccatc cagaaatcga cgtcgttgcc
     6121 ggaacggcca ttgcggcaga ggcggcaatg ggggaagggc gcaacctgaa aacgccgctt
     6181 accgtggtgt cgttttatct ttcacatcag gtgtatcgcg ggttgaagcg gggaagagtg
     6241 attatggcag ccagcgatca aatggtctgg cagggggaac tggcggttga gcaggctatc
     6301 aggcaattac aggggcaatc ggtgtctgat aatgtcagcc caccgatttt agtcatgacg
     6361 ccgaaaaatg ccgaccgcga acatatccgc cgctcgctgt caccaggggg atttcgtccg
     6421 gtttattttt atcagcacac atcagcggct aagaaataac cttcgccatg ttgtgtcacc
     6481 agtaaatccg cactgagttt atggcgtaaa cgacgaatta acacatcgac ggtgcgcagg
     6541 tcagggtttt ccacccgacg cgcagagagc atacgcagca gacgttcacg gctgagaatt
     6601 tcgcccggat tagtcacaaa tgccaccagc atttcatact ctgcgcgggt cagtttaatc
     6661 ggctcgccat cccgctccag cgtatggcgc gacacattca ggcaataacc ggcaaagcga
     6721 tagcagttgt cctgagtgtg cggttgcgcc tgtcgcgcga ggtcgattcg ccagagcaaa
     6781 tttttcactc gtaccaccag ttcgcgcagc tccagcggtt tggtgacgta atcgtctgcg
     6841 cccatttcca gcccaacaat acggtcaatc cgatcgctgc gtccggtaac cagaataatc
     6901 cccaccgttg agcgttctcg cagggcgcgg gttaacatca ggccattttc atcgggcaaa
     6961 ttgatatcca gcagaatcaa atccaccggc tgattctgca taatttcccg cagcccggca
     7021 ccgctcgccg taacggaaac ggtatacccc tcctgagtga agtaagattg taatcgcgcc
     7081 tgggtaaccg gctcatcttc aacaataaca atgtgatgtg gcatcagagg gttttactca
     7141 ttctgttcat atctgttcat attctgccgt aagccgttca tcctgaccag tgccgctgtt
     7201 catatttgct cattaagatc gcttcactaa accataattt tacaggggct attatgcgga
     7261 aactctggaa cgcgctacgc cgacccagtg ctcgttggtc ggtactggcg ctggtcgcta
     7321 ctgggattgt gattggcatt gcgctgattg tattgccaca cgttgggatc aaagtcacca
     7381 gcacaaccga attttgtgtc agttgccaca gtatgcaacc ggtgtatgaa gaatataaac
     7441 agtcggtgca tttccagaac gcctccggcg tgcgagctga atgccatgac tgtcatatcc
     7501 cgccggatat tccaggcatg gtgaagcgca aactggaagc gagcaacgac atttaccaga
     7561 cctttattgc ccactccatt gatacacctg agaaatttga agccaaacgc gcggaacttg
     7621 ccgagcgtga atgggcgcga atgaaagaaa acaactcggc aacctgccgc tcctgccata
     7681 actacgatgc gatggatcat gcgaagcagc atcctgaagc ggcgcgtcag atgaaggtgg
     7741 cagcgaaaga taatcaatcc tgcatcgact gtcataaagg tattgcccac cagttaccgg
     7801 atatgagtag tggcttccgt aagcagttcg atgagctgcg cgccagtgct aatgatagtg
     7861 gtgacacgct gtactccatt gatattaagc cgatttatgc ggcgaaaggc gataaagaag
     7921 cgtctggttc tctgctgcct gcttcggcag tgaaagtcct taaacgtgac ggcgactggc
     7981 tgcaaattga aatcactggc tggacggaaa gtgcaggacg tcagcgtgta ctcacccaat
     8041 tcccaggtaa acgcatcttt gttgcctcga ttcgtggtga tgtgcagcag caggtgaaaa
     8101 cgctggagaa aaccaccgtt gccgacacca ataccgagtg gagcaagttg caggctactg
     8161 cgtggatgaa gaaaggcgac atggtgaacg atatcaaacc gatctgggct tatgcggatt
     8221 ctctgtacaa cggcacctgt aaccagtgcc atggcgcacc ggaaatctcc cactttgacg
     8281 ctaacggttg gatcggcacg ctcaacggca tgattggctt taccagcctc gataaacgtg
     8341 aagaacgcac cttgttgaaa tatctgcaaa tgaatgcgtc tgacaccgca ggtaaagctc
     8401 acggcgataa gaaggaagaa aaataatgaa caataacgat ctctttcagg catcacgtcg
     8461 gcgttttctg gcacaactcg gcggcttagc cgtcgccggg atgctggggc cgtcattgtt
     8521 aacgccgcgc cgtgcgactg cggcgcaagc ggcgactgag gctgtcatct cgaaagaggg
     8581 cattcttacc gggtcgcact ggggggctat ccgcgcgacg gtgaaggatg gtcgctttgt
     8641 ggcagcaaaa ccgttcgaac tggataaata tccgtcgaaa atgatcgccg gattgccgga
     8701 tcatgtacac aacgcggcgc gtattcgtta cccgatggta cgcgtggact ggctgcgtaa
     8761 gcgccatctc agcgacacct cccagcgcgg tgataaccgt tttgtgcgcg tgagctggga
     8821 tgaagccctc gacatgttct atgaagaact ggaacgcgta cagaaaactc acgggccgag
     8881 tgccttgctg accgccagtg gttggcaatc gacggggatg ttccataacg cttcggggat
     8941 gctggcgaaa gctattgcct tgcatggtaa tagcgtgggt acgggcggag attactctac
     9001 cggggctgcg caggtgatcc tgccgcgcgt ggtaggttcg atggaagtgt atgaacagca
     9061 aacctcctgg ccgctggtat tgcagaacag caaaaccatt gtgctgtggg gctccgattt
     9121 gctgaaaaac cagcaggcga actggtggtg cccggatcac gatgtttatg aatattacga
     9181 gcagttgaaa gcgaaagtcg ccgccggtga aattgaggtc atcagcatcg atccggttgt
     9241 tacatccacc catgagtatc tggggcgcga gcatgtgaag cacattgcgg ttaacccgca
     9301 aactgacgtg ccgctgcaac tggcgctggc gtatacgctg tacagtgaaa acctgtacga
     9361 caaaaacttc ctcgctaact actgtgtagg ttttgagcag ttcctgccgt atctgctggg
     9421 tgagaaagac ggtcagccga aagatgccgc atgggcagaa aaactgaccg gcattgatgc
     9481 cgaaaccatt cgtgggctgg cacggcagat ggcggcgaac agaacgcaga ttattgctgg
     9541 ctggtgcgta cagcgtatgc agcacggtga acagtgggcg tggatgattg tcgttctggc
     9601 ggcgatgctg gggcaaattg gcctgccagg tggtggtttt ggttttggct ggcactataa
     9661 cggcgcaggc acgccggggc gtaaaggcgt tattctgagt ggtttctccg gttctacgtc
     9721 gattccgcct gttcacgaca acagcgacta caaaggctac agcagcacca ttccgattgc
     9781 ccgttttatc gatgcgatcc tcgaaccggg gaaagtgatc aactggaacg gtaaatcggt
     9841 aaaactgccg ccgctgaaaa tgtgtatttt tgccggaact aacccgttcc atcgccatca
     9901 gcagatcaac cgcattattg aaggctggcg caagctggaa acggttatcg ccatagataa
     9961 ccaatggacc tcaacctgcc gctttgccga tatcgtgctg cctgcgacca cgcagtttga
    10021 gcgtaacgat ctcgaccagt acggcaacca ctccaatcgt ggcatcatcg ccatgaaaca
    10081 ggtcgtgccg ccgcagttcg aggcgcgcaa cgactttgat attttccgcg agctgtgccg
    10141 ccgctttaat cgcgaagaag cctttaccga agggctggac gagatgggct ggctgaaacg
    10201 catctggcag gaaggtgtac aacaaggcaa aggacgcggc gttcatctgc cagcgtttga
    10261 tgacttctgg aataacaaag agtacgtcga gtttgaccat ccgcagatgt ttgttcgcca
    10321 ccaggcattc cgcgaagatc cggatctcga accgctgggc acgccgagtg gcctgattga
    10381 gatctactcg aaaaccatcg ccgacatgaa ctacgacgat tgtcaggggc atccgatgtg
    10441 gtttgagaaa atcgaacgct cccacggtgg gcctggctcg cagaagtatc cgttgcatct
    10501 gcaatctgtg catccggatt tccgacttca ctcgcagtta tgtgagtcgg aaactctgcg
    10561 tcagcaatat accgtagcgg gtaaagagcc agtatttatt agcccgcagg atgccagcgc
    10621 gcgcggtatt cgtcacggtg atgtggtacg cgtctttaac gttcgtggtc aggtgttggc
    10681 tggagcagtg gtttctgacc gctatgcacc tggcgtggcg cggattcacg aaggggcatg
    10741 gtacgatccg gacaaaggcg gcgagccagg ggcgctgtgc aaatacggta accccaacgt
    10801 gttgaccatc gacatcggta cttcgcagct ggcgcaggcg accagtgcgc acactacgct
    10861 ggtggaaatt gagaagtaca acggaacagt ggagcaggtg acggcgttta acggccccgt
    10921 ggagatggtg gcgcagtgcg aatatgttcc cgcgtcgcag gtgaaatcat gaccacgctg
    10981 acagcacaac agattgcctg tgtttacgcc tggctggcgc agttgttctc ccatgaactg
    11041 gacgatgaac aactgacgca aatcgccagt gcgcagatgg ctgaatggtt ttcgttgctg
    11101 aaaagcgaac ctccgctcac tgcggcggtg aatgggctgg aaaacagtat tgccactctg
    11161 acagtacgtg acgatgcccg tctggaactg gccgctgact tttgcggcct gtttctgatg
    11221 accgacaaac aagcggcgct gccgtatgca tcggcctaca aacaggacga gcaagagatt
    11281 aaacgcttgt tagttgaggc ggggatggaa accagcggca atttcaacga accggcagat
    11341 catctggcga tctatctcga tttgttgagt catctgcatt tttcgctggg agaggggacc
    11401 gttcctgcgc gaagaatcga cggtttgcgg caaaaaacac tgacggctct gcgggaatgg
    11461 ttaccagagt ttgctgcgcg ttgtcgacag tatgacagct ttggttttta cgcggcacta
    11521 agccagttat tgctggtgtt agtggagtgt gactaccaaa aaagataatg ccgtttgtag
    11581 gcctgataag acgcggca
//
LOCUS       DADSZD010000137        11523 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.138, whole genome shotgun sequence.
ACCESSION   DADSZD010000137 DADSZD010000000
VERSION     DADSZD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.138"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1151
                     /locus_tag="J1Z28_003395"
     CDS             <1..1151
                     /locus_tag="J1Z28_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBA7718198.1"
                     /translation="AFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSL
                     ALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSN
                     HAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAG
                     FGMMANAADKVKDPEVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVA
                     QAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKPLWRQ
                     STWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILI
                     VGTLVMLLVIVGFIYSLWSQGSRALIWIIGALLLSLIVAMVMKRNKTV"
     gene            1276..1683
                     /locus_tag="J1Z28_003396"
     CDS             1276..1683
                     /locus_tag="J1Z28_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CueR"
                     /protein_id="HBA7718199.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1684..2907)
                     /locus_tag="J1Z28_003397"
     CDS             complement(1684..2907)
                     /locus_tag="J1Z28_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718200.1"
                     /translation="MFSPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVIYDPHSHGL
                     IHSLCWQDVPLIRFWSEAGCPTCAEFVYSGFAEDEQGAARFLSALENWNRPWSGITDA
                     FAALTPLFSCMADGYYLLEDRELYPTDGNGHFFWAATDHSSSNPATVAVWDPEYCSFS
                     DTAPCFLLPGQPPSHFNPERATFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATHFNDEQQTLVFPGGECLHKTELQYHIPKLTACKTLPPSAWESFGP
                     DKHISPSETWSEELKQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADII
                     LQALFSTHSPLFIDFARFVISHPAYAIYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKP"
     gene            complement(3026..3484)
                     /locus_tag="J1Z28_003398"
     CDS             complement(3026..3484)
                     /locus_tag="J1Z28_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBA7718201.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGSHVEVIAIEGITLIIRAVIA"
     gene            complement(3481..4398)
                     /locus_tag="J1Z28_003399"
     CDS             complement(3481..4398)
                     /locus_tag="J1Z28_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBA7718202.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            4544..5221
                     /gene="fetA"
                     /locus_tag="J1Z28_003400"
     CDS             4544..5221
                     /gene="fetA"
                     /locus_tag="J1Z28_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBA7718203.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDTILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            5208..5987
                     /gene="fetB"
                     /locus_tag="J1Z28_003401"
     CDS             5208..5987
                     /gene="fetB"
                     /locus_tag="J1Z28_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBA7718204.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(6050..6904)
                     /gene="cnoX"
                     /locus_tag="J1Z28_003402"
     CDS             complement(6050..6904)
                     /gene="cnoX"
                     /locus_tag="J1Z28_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBA7718205.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(6965..7774)
                     /gene="ybbO"
                     /locus_tag="J1Z28_003403"
     CDS             complement(6965..7774)
                     /gene="ybbO"
                     /locus_tag="J1Z28_003403"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBA7718206.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(7764..8387)
                     /gene="tesA"
                     /locus_tag="J1Z28_003404"
     CDS             complement(7764..8387)
                     /gene="tesA"
                     /locus_tag="J1Z28_003404"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBA7718207.1"
                     /translation="MNFNNVFRWHFPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            8358..9044
                     /locus_tag="J1Z28_003405"
     CDS             8358..9044
                     /locus_tag="J1Z28_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7718208.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVRLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEV"
     gene            9041..11455
                     /locus_tag="J1Z28_003406"
     CDS             9041..11455
                     /locus_tag="J1Z28_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7718209.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQIAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSIERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVPPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVGLGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
BASE COUNT         2587 a         2973 c         3193 g         2770 t
ORIGIN      
        1 tcgcctttgc ttttggcggt attgtggcga tgttttccgg ttatgcctat gcgcgtctgg
       61 gggcgagcta tcccagcaat ggcggtatta tcgacttctt tcgtcgcgga ttaggcaacg
      121 gcgttttttc gctggcactc tcgttattgt acctgttgac gctggcagtg agtatcgcca
      181 tggtcgctcg tgcttttggc gcttatgccg tgcagttttt gcatgaaggc agccaggagg
      241 agcaccttat tttgctctac gcgttgggga tcattgcggt gatgacgctt ttcaactcct
      301 taagcaacca tgcggtaggg cggctggaag tgatcctcgt cggcattaaa atgatgatcc
      361 tgttattgct gattattgcc ggtgtctggt cgctgcaacc ggcgcatatt tccgtttctg
      421 cgccccccag ctctggggcg tttttctcct gtatcgggat aaccttcctt gcctatgcgg
      481 gctttggcat gatggcgaac gcggcggata aagtgaaaga tccggaggtc ataatgccac
      541 gggcgtttct ggtggcgatt ggcgttacca cgttgcttta tatctcgctg gcgctggttt
      601 tgcttagcga tgtatcggca ttagagttag aaaaatatgc cgataccgcc gtagcgcagg
      661 ctgcttctcc gctcctcggg catgtggggt atgtgatcgt cgtcatcggc gctttactgg
      721 cgacggcttc agccatcaac gcaaacctgt tcgccgtgtt taacatcatg gacaacatgg
      781 gcagcgaacg cgaactgccg aagctaatga ataaacctct gtggcggcag agtacctggg
      841 gcaacatcat cgtcgtggtg ttgattatgc tgatgacggc ggcgctgaat ttaggctcac
      901 tcgccagcgt tgccagcgcc acctttttga tctgctatct ggcggtgttt gtggtggcga
      961 tccgcctgcg tcatgatatt cacgcctcgt tgccgattct tatcgttggt acgttggtta
     1021 tgttgttggt gatcgttggc tttatctaca gtctctggtc ccagggcagc cgtgcgctga
     1081 tatggattat tggcgcactc ttactcagcc ttattgtggc aatggtcatg aagcgcaata
     1141 aaaccgtata acatctctct gtgcgcagta cttcctgtat tattgtggtg gcggtcgata
     1201 ttcgcactgg caaaaaaacg tgcttgaata tttgttgaaa ccctttaaca aagcacagga
     1261 ggcgttgcgc gaacgatgaa cattagcgat gtagcaaaaa ttaccggcct gaccagcaaa
     1321 gccattcgct tctatgaaga gaaggggctg gtgacgccgc cgatgcgcag cgaaaacggt
     1381 tatcgcacct acacgcagca gcatctcaac gaactgacct tactgcgcca ggcacgccag
     1441 gtgggcttta acctggagga gagcggcgaa ctggtaaatc tgtttaacga tccgcagcgt
     1501 cacagcgccg acgtcaaacg tcgtacgctg gagaaagtag cggagatcga acagcatatc
     1561 gaagaactgc aatccatgcg taaccagctg ctggcgctgg cgaatgcctg ccccggtgat
     1621 gacagcgccg actgcccgat tatcgaaaat ctctccggct gctgtcatca tcgggcgggg
     1681 tgattaaggc ttgcgaaaat agtcgtccat tattttcgtc agctccggac gttccccgtc
     1741 atcattgatg gcaaaatcca gaaaaaaagc atctgcttgc ggcgttcggt tttgcgccat
     1801 caaccggaag gtgagggggc gataaatggc gtatgccgga tggctgataa cgaatcgggc
     1861 aaagtcaata aaaagcggcg agtgtgtgga aaacagcgcc tgcaaaataa tatcagcctt
     1921 ttcatcctcc cccaaacctt gttggatcat gctcttaata cgagtttcgc tcaaattccc
     1981 ggcctcaaca atttgccagg cttgatccag cgaggggtac cgactgacgc tctgctttaa
     2041 ctcttctgac caggtttcgg atgggctgat gtgcttatca gggccgaaac tctcccatgc
     2101 actgggcggt aatgttttgc acgcggtgag ttttggtatg tggtattgca gctctgtttt
     2161 gtgcagacat tccccgccag gaaataccag ggtttgttgc tcatcattaa aatgggttgc
     2221 cgtgctgagc accagtgttt tgagcggtcg cccttccagc gcggcggcag tagccttatg
     2281 gtgtccgtcg agcagaacgc agagatagga atcagggaga taccaggcca gagcgcgggc
     2341 gtccggttta tcgcgataaa acgtcgcacg ctcaggatta aaatgtgatg gcggctggcc
     2401 gggtagaaga aagcagggcg cggtgtcact gaaagagcaa tattcaggat cccagacggc
     2461 aacggttgcc ggattactgg aactatggtc cgttgccgcc caaaagaaat gcccgttgcc
     2521 atccgtcggg taaagttcac gatcttccag caaataataa ccatcagcca tgcatgagaa
     2581 cagcggtgtc agcgcggcaa aagcgtccgt tatgccagac catgggcgat tccagttttc
     2641 gagagcagac aaaaaccggg cggctccctg ttcatcctcc gcaaaaccgc tgtagacaaa
     2701 ttccgcacag gtagggcacc cagcctcgga ccagaaacgt attaacggga catcctgcca
     2761 gcagagcgaa tggataagac cgtgcgagtg cggatcgtaa atcacacatt gacgcagaaa
     2821 tcctttcccg gacttcaccg aaaaaatgac gcccgcagcg gtatttttca gcagacgtac
     2881 ttcaggtttt cgaaaggggg aaaacatggc tactccttag ccgaacattc aattaacact
     2941 atgtaggggg ctatatgccg gatgcggcgt aaacgcctta tccggcatgg ggtttggtac
     3001 agatttccag gtgtgataag acgcgtcagg cgatgaccgc acggataatc agcgttattc
     3061 cttctatcgc aatcacctca acatgcgaac cagcgccgag atcctcgctg gcgctgacag
     3121 gccatgaact gtcaccgacg cgcatatgac cgcgcccgtt gaccagcgga gattccagca
     3181 caaaacgtcg gccaatcaac tgctgcccgc gctggtttaa atgactgtcg ctgtgctttt
     3241 gttcgcgcac ccgccgcgac aaccatttcc accacagcca ggcggcgagc agcgtcagga
     3301 cggcaaacat taccccttgc cactcccaac ccagcggcag caaccagacc accagaccgg
     3361 taatcaccgc tgccacgccg ctccacaaca gataaccatt gccgcccaac atctcggctg
     3421 ccagcagcaa accgccgaga cttagccaga aaatatgtgg atgaacgacc attaactcca
     3481 tcatggctga gtccgtttgt tggcgctgtc tttcaccagc tcagcaatcc cggcaatcga
     3541 ccccatcagg ctgctggcct ctaatggcat catcaccact ttgctgttac tggacgaacc
     3601 gatctgctgt aacgcttcgg tgtatttctg tgcgacgaag tagttcaccg cctgaatatc
     3661 accggaggcg atggcttcag acaccatttt ggtggcgcgg gcttctgctt cagcggaacg
     3721 ttcacgcgct tcagcctgta aaaacgccga ctgacgttcg ccttccgctt tcaggatttg
     3781 tgactgtttt tcaccttcgg ctttgaggat ttccgcctga cggatccctt ccgcttcaag
     3841 gatgtaagcg cgtttggtac gttccgcttt catctgcgcg ttcattgaag agataagctc
     3901 tgcgggcggg cgcacatcac gaatttcgat acgggtgact ttaatccccc acgggttggt
     3961 ggcctcatcg acaatatgca gcagacgcga gttaatgctg tcgcgctgag agagcatttc
     4021 atcaagttcc attgaaccca gcacggtacg gatgttagtc atggtcaggt tgatgatcgc
     4081 cagctccaga ttgctgactt cataagccgc gcgcggcgcg tcaatcacct gaataaagca
     4141 cacggcgtcg atggtaacgt tggcgttatc tttcgagata acttcctggg aagggatgtc
     4201 gagcacttgc tccatcatat tgatcttgcg accaatgcga tccataaagg gcaccaccag
     4261 gcttagacct ggctgtaacg ttttggtata gcgaccaaaa cgctctactg tccactgata
     4321 gccctgcggc acgattttga cgcccgcgcc aacaatgacc agcgcgacaa aaatgagaat
     4381 agggataaag ataagcatcg gaaaaacctc ctgttgtacc gtccataatc agcaaaattg
     4441 ctgcttgatt aaacaaatta tacctgatta ctgaaagaga gttccccctt attcctgcga
     4501 aggataaact gtttttagta aaaatcagaa aaagggaaca gcgatgcagg aaaatagtcc
     4561 tttgcttcag ctacaaaacg taggatatct ggcgggtgat gcgaagattc ttaataacat
     4621 caatttttcg ctgcgtgctg gcgaatttaa gttaattacc ggtccttctg gttgtggcaa
     4681 aagtacgctg ctaaaaatag ttgcttcatt gatcagccca accagcggaa cgttactgtt
     4741 tgaaggtgag gatgtcagca cactaaagcc agaaatctac cgccagcaag tctcttactg
     4801 cgcccagacg ccgacgctgt ttggcgatac ggtatacgat aatctgatct ttccctggca
     4861 gatccgtaac cagcagcctg acccagccat ttttctcgat tttctcgaac gcttcgcttt
     4921 gccggacacc attttgacga agaatatcgc cgagctatct ggtggtgaaa aacaacgcat
     4981 ctcattgatt cgtaacctgc aatttatgcc gaaggtttta ttgttggatg aaatcaccag
     5041 tgcactggat gaaagtaata aacataacgt caatgagatg atccatcgct atgtgcgcga
     5101 gcaaaatatt gccgtgctgt gggtgacaca cgataaagac gaaattaatc atgcggataa
     5161 agtgattaca ctgcaaccgc atgccggaga aatgcaggaa gcacgctatg aactcgcata
     5221 atattactaa cgaatcatta gcactggcat taatgctggt ggtagtggca atcttaatta
     5281 gccataaaga aaaactggcg ctggagaaag atattctctg gagcgtcggg cgcgcgataa
     5341 ttcagctgat tattgtcggc tatgtgctga agtatatttt cagcgtggat gatgccagcc
     5401 tgacattatt gatggtgtta tttatctgct ttaatgcggc gtggaatgcg caaaaacgca
     5461 gtaaatatat tgctaaagct tttatctcat cgtttattgc cattacggtc ggggcgggaa
     5521 ttaccctggc ggtgctgatt ctctcagggt cgattgaatt tatcccgatg caggtgatcc
     5581 ctatcgccgg gatgatagcc ggtaacgcca tggtagcggt ggggttgtgt tacaacaatt
     5641 tagggcaacg cgtgattagc gaacagcaac agattcagga gaaactgagt cttggcgcga
     5701 cgccgaaaca ggcttcagcg atattgattc gcgacagtat tcgcgcggct ttaattccga
     5761 cggtcgattc agcaaaaacg gttggcttag tgagtttacc aggaatgatg tccgggctga
     5821 tatttgccgg gattgatccg gtgaaggcga ttaaatatca gattatggtg acctttatgc
     5881 tgctctcaac cgccagcctg tcgaccatta ttgcctgcta tttaacctat cgtaaatttt
     5941 ataattcgcg ccaccagttg gtggtgacgc aattgaagaa gaaatgatgc aatgccggat
     6001 gcgcagcatc gcatccggcg ttgtggttca tgtgccgatc aacggaaact caatacaaca
     6061 acgcatacag ctggcggcga tacttcgacg ccagtgcatc acctgtaccc agcgcagcga
     6121 ggatctcctg gaacgtttta cgcgtctgac cgtctgcggc ggtgagatct ttacgcagat
     6181 gcccaaacag taactccagc gcctcttcat tgcgcccaac ctggtgcagt tgcagtgcca
     6241 gttgcgtcgc cagtgcggca tcttctggat tctctgccac ctgctgttgc agctgttgaa
     6301 tttccggcgt atctgccgcc tgcttcagca gttcgatttg cgccaccagc ccctggtagc
     6361 gggtgtcctg atcctgtaaa ggaatggttt tcagcaccgc ttctgcatct tcagaacggt
     6421 tcagcgcaat cagcgtttct gccagcagca ggccgatctc cccgttctga ttcgacaact
     6481 gccaggcgtc tttcagcaat ggcaaggcgt cggtgtaatt gccttcctgc atcagctgca
     6541 tcgcctgctg cgctttcagc tcttcttcgc gcggcagcac tttatccagc agggcgcgga
     6601 tcgcctcttc cggttgcggc ccctggaagc cgtctaccgg ttgcccgttc tggaacagat
     6661 acacggtcgg aatcgcgcgc agaccaaact gcgcggcaat catctgctcc gcgtcgcagt
     6721 ccagcttcgc cagaataaat tgcccgttgt actgcgccgc aaggctttcc agaattgggg
     6781 ttaactgcaa acagtgctgg ctacgttcag accaaaaata gaacagcacc ggagtggtca
     6841 tcgactgttc aagaacctgt tgcaggttag attcgttaat gttgacaata ttttctacgg
     6901 acatggagtc gctctctgtt gtcgatattt tctttgacat gggggcttaa gcgcgcgctt
     6961 caactcaccc ctgcaatatt ttgtccatca cgcgccccgg cagcaggcgc ttaagcacca
     7021 ttactgccca ggtcaccagc gttaccggat agcgcatctt cggcttctcg ctaataaaag
     7081 catggcgcac tttgtcgacc accgcttccg gtcccaacgt aaagcgggcg gcgatgccgg
     7141 gattttcgac aggtttatca ctttgcgtct gattgacgtt gtcggtgaag cgagtacgaa
     7201 tgggaccagg ttcgatcagg ctgactttaa ttccgctgtg gcgcagctcc atgcgcagtg
     7261 cgtctgacca cgcctccagc gcatatttac tggccgcgta agcgccacga cccggtgtgg
     7321 agattaatcc catcaccgat gaggtcatca caatacgccc ttcaccgtgc ggcaacatcg
     7381 cgggtaacag gcgcatggtg agctggtgtg cgccgaaaaa gttggcggaa aactgctgtt
     7441 ccatctgcgc acggctgatg gtggaaaggg ggccatacat gccgaatccg gcattgttaa
     7501 agatcccata cagacaatta tcggtcaggg cgatcacttc gtcggctgcg cgatcaacac
     7561 tttctggcga atccagatcg atcaacacgc cggtaaatcc catgctgttc atgcgctcaa
     7621 catcatccgg tttccggcaa cctgccagca catgaaaacc ctggcgtttt aattcgagcg
     7681 cgctttccag gccaattcca ctggaacatc cggtaattaa gaccgatttt tgcataactt
     7741 tacctgtcag gatctccgtt gctttatgag tcatgattta ctaaaggctg caactgcttc
     7801 gccatccagt cggcaataaa cggctgggcg tcgcggttgg gatgaatacc gtcatcctgc
     7861 atccattgtg gcttgaggta gacctcttcc ataaaaaagg gcagcagcgg aacatcaaac
     7921 tctttggcga gtttggggta aatggcgcta aaggcttcat tataacggcg accatagttt
     7981 gcaggcagac gtatttgcat taacaatggt tcagcgttgg cggctttgac atcctgcaaa
     8041 atctggcgca gcgtttgctc ggtttgctgt ggttgaaaac cacgcaaacc gtcattgccg
     8101 cccagttcaa ccagcaccca gcgcggctga tgctgtttca gcagagccgg aaggcgcgcc
     8161 agcccttgtt gcgaggtgtc gccgctgatg ctggcattga ctaccgacgt tttactctgc
     8221 cacttatcat tcaacaaggc aggccaggcc gcgctggcag acattcgata cccggcgctc
     8281 aggctatcac ccagaatcaa taacgtgtcc gctgcggcgg cacggaaggt taacaggacc
     8341 aggaacagga agggaaaatg ccagcggaaa acattgttga agttcatcat cttaagaagt
     8401 ccgtcggtca gggggagcat gaactctcca tcctcaccgg agttgagctg gttgtcaaac
     8461 gtggcgagac catcgcactg gtgggcgagt cgggatcggg taagtcaacc ttgctggcga
     8521 tcctcgccgg gctggatgac ggcagcagtg gcgaagtgag actggtggga caaccgctac
     8581 ataatatgga cgaagaagcg cgggcaaagt tgcgcgcgaa gcacgtaggc tttgtttttc
     8641 agtcatttat gttaattcct acccttaacg cgctggaaaa cgtcgagctt ccggcgctgc
     8701 tgcgcggtga gagtagcgca gaaagtcgta acggggcgaa agcgttgctc gaacagttag
     8761 ggttgggtaa acgtcttgat catcttccgg cacagctttc tggcggcgaa cagcaacgag
     8821 tggcgctggc gcgagccttt aatggtcgac ctgatgtgct gtttgccgac gaacccaccg
     8881 gcaaccttga ccgccagacg ggcgataaaa ttgccgatct gcttttttcc ctcaaccgtg
     8941 agcatggcac cacgttgatt atggtgaccc acgacctgca actggcggca cgctgtgacc
     9001 gctgcttacg gctggtgaac gggcagttgc aggaggaagt atgattgcac gttggttctg
     9061 gcgcgaatgg cgctcgccgt cgctattaat tgtctggctg gcgcttagcc tggcggtggc
     9121 ctgcgtgctg gcgctgggca atatcagcga tcgcatggag aaaggtttaa gccagcaaag
     9181 ccgtgagttt atggcgggcg atagggcgtt gcgcagttcg cgcgaagtgc cgcaagcgtg
     9241 gctggaggaa gcgcaaaagc gcggcctgaa agttggcaag cagctgactt tcgccaccat
     9301 gacctttgca ggcgacacac cgcagctggc gaacgtcaaa gcggtggatg atatctaccc
     9361 gatgtatggc gatctgcaaa ccaatccccc aggcctgaaa ccgcaggcgg gcagcgtatt
     9421 gctggcccca cgcctgatgg cactgcttaa cctgaaaacg ggcgatacca ttgacgtggg
     9481 cgatgccacc ttgcggattg ccggagaagt gattcaggaa ccggattccg gttttaatcc
     9541 tttccagata gccccacgcc tgatgatgaa tctggcggat gttgataaaa ccggagccgt
     9601 gcagccgggg agtcgggtca cctggcgcta taaattcggc ggcagcgaga accagctcga
     9661 cggctatgag aaatggttgt taccccagct taaacccgaa caacgctggt acggtctgga
     9721 acaggacgaa ggcgcgctgg ggcgatcgat agaacgttcg caacagttcc tgctgctttc
     9781 ggcgcttctg accttgctgc tggcggtggc ggcggtcgcg gtggcgatga atcattactg
     9841 tcgcagtcgc tacgacttgg tggcgatcct caaaacgctg ggggcagggc gagcgcaact
     9901 gcgtaagtta atcgtcggtc agtggttgat ggtgctggcg ctttcagccg ttaccggtgg
     9961 ggcgataggc ctgttgttcg aaaacgtgtt gatggtgctg ctcaagcccg tgctacctgc
    10021 cgctttacct cctgccagcc tctggccgtg gctgtgggcg ctcggcacca tgacagtcat
    10081 ctcgctgctg gtggggctgc gaccataccg cctgttgttg gcaacgcagc ctttacgcgt
    10141 gttacgtaat gatgtggttg caaacgtctg gccgctgaag ttttatctgc cgattgtcag
    10201 tgtggtggtt gtgctactgc tcgccggact gatgggcggc agtatgctgc tctgggcggt
    10261 gctggcgggc gcggtcgtgc tggcattgct gtgcggcgtg ctgggctgga tgctgctgaa
    10321 tgtacttcgc cgcatgacgc tgaaatcgct acctctgcgc ctggcggtta gccgcctgtt
    10381 acgtcagccg tggtcaacgt taagccagct ttcggcattt tcgctctcct ttatgctgct
    10441 ggcactgttg ctggtgttgc gtggcgatct gctcgaccgc tggcaacagc agctaccgcc
    10501 agaaagcccg aactactttt taatcaacat cgccacagaa caggttccgc cgctaaaagc
    10561 gttcctcgcg gaacatcaaa tagtcccgga atcgttttat ccggtagtgc gggcgcggtt
    10621 gacggcgatt aacgataagc cgacagaagg caatgaagat gaggcgctca atcgcgaact
    10681 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    10741 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttggcct
    10801 cggcgataca gtcaccttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    10861 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    10921 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    10981 gttgacgcaa ctcaaccgcc agttccctac cattagcctg ttagacatcg gcgcgatttt
    11041 aaaacaggtc ggtcaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    11101 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgtcagcg
    11161 tcatcaggag ctggtggtgt ggcgcacact cggtgcgggg aaaaagctgc tgcgcacgac
    11221 attgtggtgt gagttcgcca tgctcgggtt tgtatccggc ctggtggccg caattggtgc
    11281 ggaaacggca ctggcggtac tgcaatcgaa agtgtttgat ttcccgtggg agccagactg
    11341 gcgattgtgg attgttctgc cgtgcagcgg tgcgctactg ctgtcgcttt gcggcggctg
    11401 gctgggtgcg cgactgctta agggtaaggc gctgttcagg cagtttgcgg ggtgacgtga
    11461 taaagccgtt tatttctacc tggtagataa acggctttat tattagtgca tcacgtaatt
    11521 aaa
//
LOCUS       DADSZD010000138        11258 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.139, whole genome shotgun sequence.
ACCESSION   DADSZD010000138 DADSZD010000000
VERSION     DADSZD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.139"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..691
                     /gene="gspC"
                     /locus_tag="J1Z28_003407"
     CDS             <1..691
                     /gene="gspC"
                     /locus_tag="J1Z28_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HBA7718210.1"
                     /translation="VKNEQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQET
                     PKLSIVLNGIVLTSNDETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFET
                     HGHYEKVTLHPGLPDIIKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFT
                     TSLLQPGDIALRINNLSLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELT
                     GMNGQRNEGTQ"
     gene            675..2627
                     /gene="gspD"
                     /locus_tag="J1Z28_003408"
     CDS             675..2627
                     /gene="gspD"
                     /locus_tag="J1Z28_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HBA7718211.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITRANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRPLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            2637..4118
                     /gene="gspE"
                     /locus_tag="J1Z28_003409"
     CDS             2637..4118
                     /gene="gspE"
                     /locus_tag="J1Z28_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HBA7718212.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERTVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFAPVS
                     PQQAQMFKYHQLVVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            4115..5311
                     /gene="gspF"
                     /locus_tag="J1Z28_003410"
     CDS             4115..5311
                     /gene="gspF"
                     /locus_tag="J1Z28_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HBA7718213.1"
                     /translation="MNYRYRAMTQDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQRTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLNNLEIRQRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            5321..5758
                     /gene="gspG"
                     /locus_tag="J1Z28_003411"
     CDS             5321..5758
                     /gene="gspG"
                     /locus_tag="J1Z28_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HBA7718214.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            5766..6275
                     /gene="gspH"
                     /locus_tag="J1Z28_003412"
     CDS             5766..6275
                     /gene="gspH"
                     /locus_tag="J1Z28_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HBA7718215.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            6272..6649
                     /gene="gspI"
                     /locus_tag="J1Z28_003413"
     CDS             6272..6649
                     /gene="gspI"
                     /locus_tag="J1Z28_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HBA7718216.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            6642..7229
                     /gene="gspJ"
                     /locus_tag="J1Z28_003414"
     CDS             6642..7229
                     /gene="gspJ"
                     /locus_tag="J1Z28_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HBA7718217.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            7222..8205
                     /gene="gspK"
                     /locus_tag="J1Z28_003415"
     CDS             7222..8205
                     /gene="gspK"
                     /locus_tag="J1Z28_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HBA7718218.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPGEGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            8220..9383
                     /gene="gspL"
                     /locus_tag="J1Z28_003416"
     CDS             8220..9383
                     /gene="gspL"
                     /locus_tag="J1Z28_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HBA7718219.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIQSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHCVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIAFWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPAAVPLLYHLQTLLLEHPELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            9380..9841
                     /locus_tag="J1Z28_003417"
     CDS             9380..9841
                     /locus_tag="J1Z28_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HBA7718220.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQEQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEVTLKHLSFREQQ"
     gene            9841..10518
                     /locus_tag="J1Z28_003418"
     CDS             9841..10518
                     /locus_tag="J1Z28_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBA7718221.1"
                     /translation="MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
                     LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLG
                     YLAWISY"
     gene            complement(10547..11023)
                     /gene="bfr"
                     /locus_tag="J1Z28_003419"
     CDS             complement(10547..11023)
                     /gene="bfr"
                     /locus_tag="J1Z28_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBA7718222.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(11095..>11258)
                     /gene="bfd"
                     /locus_tag="J1Z28_003420"
     CDS             complement(11095..>11258)
                     /gene="bfd"
                     /locus_tag="J1Z28_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBA7718223.1"
                     /translation="KKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVRAAREVMEDELM
                     QLPEFKESA"
BASE COUNT         2935 a         2890 c         2691 g         2742 t
ORIGIN      
        1 agttaaaaat gaacagatca ttaatcaacc aacaaatgca tttcagtctg aattcagcct
       61 tgccgcttta tggcgcaacg aaaatcatgc tggggtaaaa gatgcaaatc ccgtggcagt
      121 taatcaggaa acacctaagc tctctatagt gcttaatggc attgtactca catcgaatga
      181 tgagacttct tttgtcttaa ttaatgaggg gaacgaacaa aagcgatact ccctcaacga
      241 agcccttgag tcagcgccgg gaacgtttat tagaaaaata aataaaacca gcgttgtgtt
      301 tgaaactcac gggcattacg aaaaagtaac tctccatcca gggctacctg acatcatcaa
      361 gcagcctgat tcggaaaatc aaaatgtact cgccgattat attatcgcca cacctatacg
      421 tgatggagaa cagatatacg gtcttcgatt gaatcctcgt aagggtctca atgcatttac
      481 caccagcttg ctgcaacctg gggatatcgc cctcaggatc aataatcttt cgctgaccca
      541 ccctgatgag gtttcacagg cattaagttt actgctaact caacaaagtg ctcagtttac
      601 aattcgtcgc aacggcgtac cccgcttgat aaatgtttcc gtcgcggaac ttacaggaat
      661 gaatggacag cgcaatgaag ggactcaata aaatcacctg ctgcttgctg gcagcactac
      721 tcatgccttg tgcaggacac gctgagaacg aacaatacgg cgctaacttc aataacgccg
      781 atatccgcca gttcgtggaa atagtgggtc agcatcttgg caaaacgatc ctgatcgacc
      841 cttcggtaca gggaaccatt tccgtacgca gtaatgatac gtttagccaa caggagtact
      901 accagttctt tttaagtatt cttgatcttt acggttattc cgtgatcaca ctggacaatg
      961 gttttctgaa agtggtgcgc tcagctaatg taaaaacatc gccagggatg attgctgaca
     1021 gttctcgtcc aggcgtaggt gatgagttgg tcacccgaat cgtaccgctt gagaacgttc
     1081 ctgctcgtga cctggctccc ctgctccgcc agatgatgga tgcgggtagc gtcggtaatg
     1141 ttgtgcatta tgaaccctcc aacgttctta ttctgaccgg tcgtgcctcc accattaata
     1201 aactgattga agtcataaag cgcgttgatg tcatcggcac agagaagcag caaattattc
     1261 atctggaata tgcatcagcg gaagatctcg ccgagattct taatcaatta atcagcgaaa
     1321 gccacggtaa aagccagatg cctgccctcc tttccgcgaa gattgtggcg gataagcgaa
     1381 ccaactctct tatcatcagc gggccggaaa aagcacgcca gcgcataact tcattactga
     1441 aaagccttga tgtagaagag agcgaggaag gaaatacccg ggtctattac ctgaaatatg
     1501 ctaaagccac gaatctggtg gaagtgctaa ccggtgtttc cgaaaagctg aaagatgaaa
     1561 aagggaattc gcgtaagcct tcttctactt ctgcgatgga taatgtggcc attaccgccg
     1621 atgaacagac caactcgttg gtcattaccg ctgaccagtc cgtccaggag aaactcgcca
     1681 cggtaattgc gcgtctggat attcgccgtg cacaggtgct ggttgaggca atcatcgtcg
     1741 aagttcagga tggaaatgga ctcaacctcg gcgtgcaatg ggcgaataaa aacgttggcg
     1801 cacagcaatt taccaatacg ggattaccgg tttttaacgc tgcgcaaggt gtagctgatt
     1861 ataaaaagaa tggcgggatc accagagcga atcctgcctg ggatatgttt agcgcctaca
     1921 atggcatggc tgcaggcttc ttcaatggcg actggggcgt attgttgact gcgctggcca
     1981 gtaacaataa aaatgacatc ctcgccaccc cgagcatcgt aacgctggat aataaactcg
     2041 cgtccttcaa cgttggtcag gatgtgccgg tgctatccgg ctctcagacc acttctgggg
     2101 ataacgtctt taatactgtc gaacgcaaaa cggtgggcac aaaactcaaa gtcactccgc
     2161 aggtcaatga aggcgacgcg gtactgctcg aaatagagca ggaggtttcc agcgttgact
     2221 cttcgtctaa ttcgacgctc ggcccgacat ttaacacccg tactattcaa aacgccgtgc
     2281 tggttaaaac cggtgaaacg gtggtcctgg gcggattgct ggatgatttt tctaaagagc
     2341 aagtgtcaaa ggttcctctg cttggcgata ttcctttagt gggacaactc ttccgctata
     2401 cctcgaccga gcgcgctaaa cgcaacctga tggtatttat ccgcccgacg attatccgtg
     2461 acgatgatgt ttatcgccca ctgtcaaaag agaaatacac ccgctaccgt caggagcaac
     2521 agctgcgaat cgacgggaaa tcaaaagcgc tggttggctc ggaagatttg ccggtgctga
     2581 atgagaacac gttcaacagt cacgcccctg cgccatcgtc acggtgaggc attcttatga
     2641 gaattcactc accgtacccc gccagttggg cgctggcaca acgaattggt tatctctatt
     2701 cagagggcga gattatttat ctcgccgata cgccattcga acggttactc gatattcaac
     2761 gtcaagttgg ccagtgccag accatgacca gcttgtcaca ggctgatttt gaagcccgac
     2821 tggaagcggt gttccatcag aataccggtg agtcgcaaca gattgcgcag gatatcgatc
     2881 aatccgtcga tcttctctcg ctttcggaag agatgcccgc aaatgaagat ctcctgaatg
     2941 aagattcagc ggcaccggtt atccgcttga tcaatgctat tttgagtgag gccatcaaag
     3001 aaaccgcctc cgatatccac attgaaacct atgaaaaaac aatgtcgatc cgttttcgca
     3061 tcgacggcgt tttgcggaca attttacagc ccaacaaaaa actggcggca ctgcttatct
     3121 cccgaattaa ggtcatggcg cgtcttgata tcgccgaaaa acgtattcca caggatggaa
     3181 gaatcagttt gcgtatcggg cgacgtaaca tagatgtccg cgtatccaca ctgccatcca
     3241 tctatggtga acgcactgtg ctccgcctgc tggataaaaa cagcctccag ctttcattga
     3301 acaacctggg gatgacggca gcggataaac aggatttaga aaatctcatt cagcttccgc
     3361 acggtattat cctggtaaca gggccgacag gctccggtaa aagcaccacg ctctacgcca
     3421 ttctttcggc gcttaatacc cctggccgta atattctgac ggtagaagat cccgtggaat
     3481 atgaactgga aggcatagga caaacgcagg tgaatacccg agtggatatg tctttcgctc
     3541 gcggcctgcg cgccatactt cgccaggacc cggatgtcgt catggtgggg gagattcgtg
     3601 atacagaaac cgcgcaaatt gcggttcagg cctcgctcac aggccatctg gtactctcaa
     3661 cactccacac taacagtgca tcaggcgcag tcacccggct acgcgacatg ggcgttgaat
     3721 cattcctgct ttcgtcttca ctggcaggga ttatcgcgca acgtctggtt cgtcgtctgt
     3781 gtccgcaatg ccgacaattc gcgcccgtat cccctcaaca agcgcagatg tttaaatatc
     3841 atcagctcgt ggtgacaaca attggcactc ccgtaggctg ccctcattgc catcaatccg
     3901 gctatcaggg gcgcatggcg atccacgaaa tgatggtggt gacgccggaa ttgcgggccg
     3961 ctattcatga aaatgtggat gaacaagcac tggagcgact ggtccggcaa caacacaatg
     4021 ccttaatcaa aaatggcctg caaaaagtga tacgcggtga cacctcctgg gatgaggtta
     4081 tgcgcgtcgc cagtgccacg ctggagaacg aagcatgaat tatcgctatc gcgccatgac
     4141 ccaggatggt caaaaattgc aggggatcat tgatgctaac gatgaacgtc aggcacgact
     4201 gcggctgcgt gaagaagggc ttttcctgtt ggatattcgc ccccaaaaaa gttcgggagt
     4261 aaaaacacgt cgcccgagga tcagccatag tgaactgacg cttttcaccc ggcagttggc
     4321 aaccttaagc gcagcggcat tacctctgga agagagcctt gccgtaattg gtcaacaaag
     4381 cagtaataat cgactggctg acgtgttaaa tcaggtacgc agcgccatcc ttgaagggca
     4441 tcccctttcc gatgcgttac aacattttcc cacgcttttc gattcgctct accgtaccct
     4501 ggtaaaagcg ggcgaaaaga gcggtctgct ggccccggtg ttggaaaagc tggctgatta
     4561 caatgaaaac cggcagaaaa tccgcagcaa gctcattcag tcactgatct acccctgcat
     4621 gctcactacg gtggcgattg tggtcgtgat tattctcctc actgctgtcg tgcccaaaat
     4681 taccgaacag ttcgtgcata tgaagcagca actgccactg agtacacgca ttcttttagg
     4741 tctgagcgac acgttgcaac gtaccggccc gacattatta gcgacagtat ttattgtcgc
     4801 tgtgggtttc tggctctggt taaaacgcgg caataaccgc caccgttttc atgccatgtt
     4861 actgcgcgtt gcgctcatcg gcccgctgat ttgcgccatt aacagcgcac gctatctccg
     4921 cactttaagt attttgcaat ccagcggcgt ccctctgctg gatgggatga atttgtccac
     4981 cgaaagcctc aacaacctcg aaattcgcca gcgcctggca aatgcggcag aaaacgttcg
     5041 ccagggtaac agcattcatc tttcactgga acaaaccgca attttcccgc cgatgatgct
     5101 ctacatggtg gcctctggcg aaaaaagcgg gcagctcggc acgttaatgg tcagagcagc
     5161 agataaccag gagacactcc aacaaaatcg gatcgcctta acgctctcca tcttcgagcc
     5221 agcactcatt attacgatgg cactgatcgt cctgtttatt gtcgtgtcgg tactccaacc
     5281 tcttcttcaa cttaactcaa tgattaatta aggaaatcat atgcgcgcaa cggataagca
     5341 acgcggtttt acattactgg aaattatggt ggtgatcgtc attatcggcg tacttgccag
     5401 cctggtggtt cccaacctga tgggcaataa agaaaaggcg gataaacaaa aagccgtcag
     5461 cgatattgtg gcgctggaaa acgccctgga tatgtacaag ctcgacaatc atcgctatcc
     5521 aaccacaaat caggggcttg aatccttagt cgaagcgccg acactgccac cgctggctgc
     5581 aaactataac aaggaaggtt atatcaagcg tctgcccgcc gatccctggg gcaatgatta
     5641 tgtccttgtt aatcctggtg aacacggcgc atacgatctg ctttcagcag ggccggacgg
     5701 tgaaatggga accgaggacg acatcaccaa ctggggtttg agcaagaaga aaaaataagt
     5761 cagcgatgaa tcagcaacgc gggtttaccc tgctggagat gatgctggtg ctggcgctgg
     5821 tcgctatcac ggcaagtgtg gtgctcttca catatggtcg ggaggatgcc gccagcactc
     5881 gtgcgcgcga aacagcggcg cggtttaccg ctgcacttga attagccatc gaccgcgcca
     5941 cgctcagtgg tcagcctgtc ggtatccatt tctctgattc agcatggcgc attatggtac
     6001 cgggcaaaac accatcagcc tggcgctggg ttccattaca agaagatgcc gcagacgaga
     6061 gcaaaaatga ctggggtgaa gaactctcaa ttcaacttca gccatttaaa cctgatgaca
     6121 gcaaccaacc gcaggtggtg attctggccg acggacaaat cactcccttc tctttgttga
     6181 tggctaatgc cgggaccggt gagccgctcc tgacgttagt cagttcaggc tcatggcccc
     6241 tggatcaaac gctcgccaga gatacccgcc catgaacaaa caatcaggga tgacactgct
     6301 tgaagtctta ctggcgatga gtattttcac cgctgttgcg ctgactttaa tgagcagtat
     6361 gcaagggcaa cgaactgcca tcgagcggat gcgtaatgag acgctggcat tgtggatagc
     6421 ggataaccag cttcagtcac aagattcatt cgacgaggaa aacacgtcca gttctggcaa
     6481 agagttaata aatggcgaag agtggaactg gcgcagcgat atccactcaa gcaaagacgg
     6541 cacgcttctg gaacacacca taacggtaac attacccagc ggtcagacaa cctcgcttac
     6601 gcgctatcaa agcatcaata ataaatccgg gcaggcacaa gatgattaat cgccagcagg
     6661 gtttcacatt gctggaagtc atggcggcgc tggcgatttt ctcaatgctt agcgtgctgg
     6721 cgtttatgat tttttctcaa gcttccgagt tgcatcaacg ttcacagaaa gaaatccagc
     6781 agttcaatca gctacagcgc accatcacca ttcttgataa cgatctcctg caactggtcg
     6841 cccggcggaa tcggagtacg gacaaaatca tggtgttggg tgaagaagcg atttttacca
     6901 cgcaaagtcg cgatccgctg gcccctctca gtgaagcaca aaccttactc actgttcact
     6961 ggtatctgcg aaatcatacg ctctaccgtg ctgttcgtac ttctgtggat ggcaggaagg
     7021 atcaacccgc ccaggcgatg ctggaacatg tcgagagctt tcttctggaa agtaacagcg
     7081 gagaaagcca ggagcttcct ctctcagtca cgttacatct gaagactcag caatatgggg
     7141 cgttacagcg ccgttttgca ttacctgagc agttagcgag agaagagtcg ccagctcaga
     7201 cgcaggccgg gaataataac catgaataat gaacaacgcg gcgtagcact gctcattgta
     7261 ctcatgttac tggcgctcat ggctgctctg gctgcagaca tgacgatcag ttttcacggc
     7321 cagcttcatc gaacccgcca ggtaaaccat catttgcaac ggcaatatga tattgagctg
     7381 gcggagaaac ttgcgcttgc cagcctcacc ctggacgtta aagataacga cagacaaacc
     7441 acgttgcagc agtactgggc acaaccgcaa cagttgcagc tggaaaatgg caatacagtg
     7501 aagtggcagt tacgcgatgc ccagcactgt tttaatctca atgccttagc aaaaatctct
     7561 gacgccccgc tggcttcccc tgatttcccc gcccaggtat tcagtgccct gctgattaac
     7621 gccggtatcg atcgcggaaa caccgatgag atcgtgcaat caattgctga ctatattgat
     7681 gccgatgatt cgccacgctt tcacggcgca gaagataact tttaccaaag ccaaacgccg
     7741 cctcgccata gcgccaatca gatgcttttt ctgaccggtg aattgcgtca gataaaaggc
     7801 ataaccgaaa acatctatca gcggcttatc ccgtatgtct gcgttctccc gacctcggaa
     7861 cttagcatca atcttaatat gctaacggag aacgatattc cgctcttcag agcgttattc
     7921 ctcaataaca tcacggatgc tgacgctcgg gtcttattac aaaaaaggcc cggggaaggc
     7981 tggctaacca cagatgcatt tctttactgg gcgcagcagg atttctccgg cgtgaagccc
     8041 ctggttgctc aggtgaaagg gcatctcttt ccctatagcc gctattttac gctgagtacg
     8101 gaaagtatca gcgacgaaca atcccaggga tggcaaagcc atattttttt caaccgtaaa
     8161 cagcagagcg cccaaatcta tcggcgtacg ctgcaactct actaatcata aggatcgtga
     8221 tgcctgaatc gttgatggta atacgttctt tctcgacgct gcgtaagcat tgggaatgga
     8281 tgacgttctc cgcagatagc gtatcttcgg tacatacgct cacagacgat cttccgctgg
     8341 agtcgctggc cgatcagccc ggtgccggaa atgttcacct tttgatccca cctgagggtc
     8401 ttttatatcg ttccctcacg ctgcccaacg ccaaatacaa actcaccgcg caaacattac
     8461 agtggttggc ggaggagaca ttacccgacg ccagccaaaa ttggcactgg acggttgtcg
     8521 ataaacaaaa cgaaagtgtc gaggtgatag gcattcagtc ggagaaactc agtcgttatc
     8581 ttgagcgttt acatactgcc ggactcaacg tgacgcgggt tttgccagat ggctgctatc
     8641 ttccctggga agtagatagc tggactctgg ttaaccagca aacaagctgg cttattcgca
     8701 gtgccgcaca tgccttcaac gaactggatg aacactggtt acagcatctt gctaaccagt
     8761 ttccgccaga gaatatgcgt tgttatggtg tagcgcctca cggcgttgca gcggccaatc
     8821 cacttatcca gcatcctgaa ataccatcac tgagtttgta ttctgccgat attgcattcc
     8881 agcgctatga catgctgcat tgtgtttttc gtaaacagaa aactgtaagc aaatcgggaa
     8941 aatggctcgc ccgcctggca gtaagctgtc tcgttctcgc cattctttct tttgttggca
     9001 gccggggcat cgcgttctgg caaacgctta agattgaaga tcaacttcag caacaacagc
     9061 aagaaacctg gcaacgttat ttcccgcaga tcaaacacac ccacaacttt cgtttttact
     9121 ttaagcagca actcgctcag caatatcctg cagctgtacc gttgctctat catttacaaa
     9181 cacttctgct tgaacaccct gaactgcagt taatggaggc gaactatagc cagaggcaaa
     9241 agtcgctgac cttaaaaatg agtgctaaga gtgaagccaa tatcgatcgt ttctgtgagt
     9301 taacacagtc ctggcttccg atggaaaaaa cagaaaaaga tccggtcagt ggtgtatgga
     9361 cagtaaggaa ctcagggaaa tgattaaatc atggtgggca gaaaaatcca catcggaaaa
     9421 acagatcgtt gcggcattgg cggttctctc gctcggcgtt ttttgttggc ttgcggtgat
     9481 aaaaccgata gatacctaca ttgaagagca tcagtcccac gcgcaaaaaa ttaagaagga
     9541 catcaaatgg atgcaggaac aagcgagcac ccatggttta ctcggtcatc cagcgcttac
     9601 gcagcccatc aaaaatatcc ttctcgaaga agcaaaacgt gagaatttga ctatcacgct
     9661 ggaaaacggt ccggacaata ctctgacaat taatcctgtc acagctcccc tcgaaaacgt
     9721 ctcccgctgg ctcactacgg cacagataac atacggtatt gttattgaag accttcagtt
     9781 tactcttgcc ggaaacgaag aggtcaccct caagcatttg tccttcaggg agcaacaata
     9841 atgacaatgc tactgccgct cttcattctg gttggtttta ttgcaggtta ttttgttaat
     9901 gccatggcct atcacctctc gcctttggag gataaaacgg cgttaacgtt tcgccaggta
     9961 ttggttcatt tctggcaaaa aaaatatgcc tggcatgata cagtgcccct gatactttgc
    10021 gttgctgctg ctatcgcctg cgccctggca cctttcacgc ccattgtgac tggcgcactc
    10081 tttctctatt tctgtttcgc actcacgctc agtgttattg attttcgcac tcagctcctg
    10141 cccgacaaac tcaccttacc gctgctctgg cttggcttgg tatttaatgc gcagtctgga
    10201 ttaattgatt tacatgatgc ggtttatggc gcggtcgctg gctatggggt gctgtggtgt
    10261 gtttactggg gcgtctggtt agtttgtcac aaagagggat tgggctacgg tgatttcaag
    10321 ctactggccg ctgcaggcgc gtggtgtggc tggcaaacgt tgccaatgat actgctgatt
    10381 gcctcgctgg gtggcattgg ttacgccatc gtttcacaac ttctgcaacg ccggactata
    10441 accactatcg cattcggccc gtggcttgcg ctcggcagca tgataaacct gggatatctg
    10501 gcctggatct cttattaacc gggagggttc tccctcccga cacggctcaa ccttcttcgc
    10561 gaatctgtgc ttgcagataa ttttgcagtc ccatcttctg aatcagatca agttccgttt
    10621 ccagccagtc gatatggcct tcttcatcgc gcaaaatttc tatcatcata tcgcggctga
    10681 cgtaatcatg aacgctatcg gcataaccaa ttgcctcacg caaattcttc gcgccatcca
    10741 gctcaagtgc cagatcagaa cgcagcattt cctcaacatc ttcaccaatg ttcagtttgc
    10801 ccaggtcctg taagtttgga agaccttcca gaaaaagaat gcgctcaata taacgatcgg
    10861 cgtgtttcat ctcatcaatg gattcatgat actccacatc attgagacgt ttgagacccc
    10921 agtttttaaa catccgggca tggagaaagt actgattgat tgcgacaagc tcatttccca
    10981 acagtttgtt gagataattt ataactttag tatcaccttt cattttttga ctccttcgct
    11041 tccactcttt gaagcgtaga acggcttacg agtaagtcaa aaaacacact ccgtttatgc
    11101 ggactcctta aactccggca gctgcattaa ttcgtcctcc atcacttcac gcgcggcccg
    11161 gacacattta ccgcactgat tgccgaccgg aatgaatttt tttaattgct ggaacgagtg
    11221 gggggaaaac tggcgcacag cctgacgaat ttttttat
//
LOCUS       DADSZD010000139        11253 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.140, whole genome shotgun sequence.
ACCESSION   DADSZD010000139 DADSZD010000000
VERSION     DADSZD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.140"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(53..874)
                     /gene="dapB"
                     /locus_tag="J1Z28_003421"
     CDS             complement(53..874)
                     /gene="dapB"
                     /locus_tag="J1Z28_003421"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBA7718224.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAVLEREGSFL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(1035..1400)
                     /locus_tag="J1Z28_003422"
     CDS             complement(1035..1400)
                     /locus_tag="J1Z28_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBA7718225.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIAVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(1423..2337)
                     /gene="rihC"
                     /locus_tag="J1Z28_003423"
     CDS             complement(1423..2337)
                     /gene="rihC"
                     /locus_tag="J1Z28_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBA7718226.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLEIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(2403..3353)
                     /gene="ispH"
                     /locus_tag="J1Z28_003424"
                     /gene_synonym="lytB"
     CDS             complement(2403..3353)
                     /gene="ispH"
                     /locus_tag="J1Z28_003424"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBA7718227.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(3355..3804)
                     /gene="fkpB"
                     /locus_tag="J1Z28_003425"
     CDS             complement(3355..3804)
                     /gene="fkpB"
                     /locus_tag="J1Z28_003425"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBA7718228.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(3912..4406)
                     /gene="lspA"
                     /locus_tag="J1Z28_003426"
     CDS             complement(3912..4406)
                     /gene="lspA"
                     /locus_tag="J1Z28_003426"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HBA7718229.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(4406..7222)
                     /gene="ileS"
                     /locus_tag="J1Z28_003427"
     CDS             complement(4406..7222)
                     /gene="ileS"
                     /locus_tag="J1Z28_003427"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBA7718230.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLLGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(7265..8206)
                     /gene="ribF"
                     /locus_tag="J1Z28_003428"
     CDS             complement(7265..8206)
                     /gene="ribF"
                     /locus_tag="J1Z28_003428"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBA7718231.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(8214..8432)
                     /locus_tag="J1Z28_003429"
     CDS             complement(8214..8432)
                     /locus_tag="J1Z28_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 domain-containing protein"
                     /protein_id="HBA7718232.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            8535..8798
                     /gene="rpsT"
                     /locus_tag="J1Z28_003430"
     CDS             8535..8798
                     /gene="rpsT"
                     /locus_tag="J1Z28_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBA7718233.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(8894..9799)
                     /gene="nhaR"
                     /locus_tag="J1Z28_003431"
     CDS             complement(8894..9799)
                     /gene="nhaR"
                     /locus_tag="J1Z28_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBA7718234.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(9859..11025)
                     /gene="nhaA"
                     /locus_tag="J1Z28_003432"
     CDS             complement(9859..11025)
                     /gene="nhaA"
                     /locus_tag="J1Z28_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBA7718235.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         2859 a         3141 c         2684 g         2569 t
ORIGIN      
        1 atattcaatg aattaaatgt gttatttttg catcaccaca aatatcttgt ggttacagat
       61 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
      121 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggatgcctta tgggtgatct
      181 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
      241 gcacggtggc aaaaccaatg gtgccaggca cacgttcacc ggtatggcct tcacgactgt
      301 agaccgcgca atctttcaga tctttatcaa gagcgtgggc gatcgcctct cccattgcca
      361 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
      421 cggtgtagtc ccccatcact ttggctgctt tctccagcag tttaagcatg acgttaacgc
      481 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaatcgcct
      541 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctggcgac
      601 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
      661 catcttttat cgcatcaaga ctactttgca cagtaacgcc tgttttcccg gctccggcta
      721 gttcgcccgc atcgctgccc agtaaaaaag atccttcgcg ctccagcacc gcgcccagct
      781 gcacgccttc taatgccagc gccgcctgaa tcaactggcg gcccatacgg cccccggctc
      841 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
      901 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
      961 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgacgcagaa agtgatgcgt
     1021 cataccctct ggcattatgc ctggtcttgt gactttgacg gcaggcagca cagtataagc
     1081 agaattacgc ccgtaacagg gatcaacacg gcaatggtaa cccaccaccc tgaatatccg
     1141 atatcgtgca tccttctgac ggctaacgct atcgccggta cggcaaaaat caacgttaaa
     1201 ataaatgacg cgggcagcgc aaaaaaaatg aaaacaccat agtcattgtt aattgttacc
     1261 gctaaccacc aacataaaag cggaagcaga cagataacgc cactaccaat cataaacgac
     1321 cagaactcga agcgattgga aacaccttca taattaaaga tgtttttcca cccttccaga
     1381 tacgcttctc catacgtcat agaacacttc cttgttttat tattagagcg ccagggccag
     1441 cacctcagcc acccactgct ggaagccttt cacatccaga tccagcgcca cctgtacatt
     1501 ggctggtttg cccaggcaac cgtcgatatc aaccaccgtc gtgcctgagg taaattcgcc
     1561 ctgagtttcc actgccacaa aacacggttt gagagtgaac aattccgggc gcaccagcca
     1621 ggcgatagcg caaagatcgt gcatacgcag tccactttgc atactgccgc tacggtagtg
     1681 gctaaacagg gcgtggagca tttttccggt gcgattcaac tccggcaaag tagcgagata
     1741 gtcaggggtt aatattgcct gattggtgac atccagacca cacatgacga tttcgatgcc
     1801 gctacggaag acacaggctg ccgcttctgg atcggcagcg atattaaact cggcgtttgg
     1861 tgtgcagttg ccgcgaccgg cagaaccgcc catgatcacc agacggcgaa tatacggctt
     1921 acattccggg cattgtgaaa gtaacagcgc aatattggtt aacgggccga tggccaccag
     1981 ggtaacaggc tcaggcgcac gcatcagggc atcccgaatc gccagaaacg ccggtatctc
     2041 cagtggcttt cggttgtgct caacaaagtc gtagcctgcc attcccgatt cgccgtgcac
     2101 agatgccgca tcacgcggtg cgcgtaccag cggcacagct gcgccctggg caagcggaat
     2161 ctccgcattc cagaaatgca gcagttgcag ggcgttgcgg gtggttttct ctaccgagac
     2221 attacccgcg acggtagtca tcagttgcag gtcgagttcg ggagcaaaaa tcgcggctgc
     2281 aatggcgacg gcatcgtcaa tgccggggtc tgtatcgagg aagataggta aacgcatgtt
     2341 ttctccataa aaaatgccgg taacaagacc ggcattttcg cataacttag gctgctaatg
     2401 acttaatcga cttcacgaat atcgacacgc agctctttcg gcacttcgaa aacaatgttt
     2461 tcttcacggc cttccagcgg aatggcttca ccaccgccca gctgctgcaa acgtgccacc
     2521 acattctgca ccagaatatc cggagccgat gcgcccgcag tcacgccgac gcatttaacc
     2581 tctttcaccc actcttcctg gatatctttc gcatcgtcaa tcaaaaacgc gcgtttgccc
     2641 atgcgctggg ccagttccgc cagacggttg gagttggagg agtttttcga accgaccacc
     2701 agcaccactt ccgcctgctc tgccaaggcg cgtaccgctt cctgacggtt agtcgtggcg
     2761 tagcagatgt catctttgcg cggaccgaca attttcggga agcgtttacg cagcgcgtcg
     2821 atcacatcag acgtgtcatc caccgacagc gtggtttggg tcataaagga gagcttctct
     2881 tcgtttttga ccgtcagttt ccacacatcg tctggcgatt cgaccagata cattccccct
     2941 tccgggttgc tgtactggcc catcgtccct tccacttccg ggtgcccggc gtgaccgatg
     3001 agaatagatt cttcaccacg acggctggcg cgggcgactt ccatatgcac tttggtcacc
     3061 agcggacagg tggcatcaaa caccgtcaaa tcgcggcttt tcgcttcgtt acgtactgcc
     3121 tgagaaacac cgtgtgcgga gaaaatcagg atcgcgccgt ccggtacttc gctaatctgc
     3181 tcaataaaga tagccccacg ctcgcgcagg ctatcgacca cgtagcggtt atgcaccact
     3241 tcgtgacgga catatatcgg tgcgccgtaa atggccagcg cgttttcaac aatgctgata
     3301 gcgcggtcta ccccggcaca aaaaccacgc gggttggcca acaggatctg catgttacgc
     3361 ctccagtgcc ggatcgattt ccagcacttc aatatcaaaa tgaacggtct gcccggccag
     3421 cggatggttg aaatcaacgg taatggagtc gccgttaatt tcgcggatca cgccaggcat
     3481 ctcactgcca tccattgcgg taaaaagcat gattgcgcca atttctggct cgcctgcatc
     3541 cataaattca cggcgggaga agtactgaat caggtccggt gacggcacgc caaacgccgc
     3601 atctggctcc agcgagaagg tggttttatc gcccactttc agccccagca ggtgttgctc
     3661 cagcccttca gaaagagaag catcgcccag gcggaacagc gccggtttac cgttgttgcg
     3721 ggtagactcg gcggtggtgc catcgtcgag tttcagcgtg aagtgcacca ggacggcgct
     3781 attgctctgt acagattcag acatgcaggt ttgctcttat attttgagga tttttgccgg
     3841 atgcggccta cgatttcgca gcaatctgta ggccggataa gatgcgtcag catcgcatcc
     3901 ggcagggttt attattgttt tttcgcttta gaaggtaaaa aaccttccag cacaatcagt
     3961 gccgcaccga cacaaatggc agtatcggca aggttgaagg tggcgaagtg ccagtcgccg
     4021 acatagaagt cgatcatatc gacaacgaag ccgtgccaca ggcggtcgaa caggttgccc
     4081 agcgcgccgc caataatcag cgcgtaagcg atattgttta gcttctgcgt ggccttcgag
     4141 cgatacatca tcactgccag gatcacgcta ataccaatcg caataccggc aaaaaaccaa
     4201 cgctgccagc cgccgctatc ggcaaggaaa ctaaacgccg cgccatagtt acgcgcataa
     4261 tgcagattaa gcgacgggaa cagcgggacc gtatccccca gagcaaagtt ctggaggatc
     4321 aggtatttgc tgcccagatc gataatcagc acgactacca ccagccacag ccagcgtagc
     4381 cctgttgaac agatcgattg actcatcagg caaacttacg tttttcaccg tcaccggcga
     4441 cgttgctgac acagcggccg cagatttctg cgtgttccgc caccttgccg acatcctggg
     4501 tgtagtgcca gcagcgaggg cacttctcac cttcggcttt actcaacgcg actttcagac
     4561 ctttgagtac ttcgctctgc tgagcatcag caggtgcgtc gttatagtct gcaacggtag
     4621 cgccggaggt caacaggaca aatcgtaatt catcgcccag cgcggtcagt ttcgccgcca
     4681 gttccggttc tgcatacaag gttactgccg cttccagcga gccgcccact ttcttatcgg
     4741 cacgcgcttg ctcaatgact ttgttcactt cgccacgcac tttcaacagc tcgtcccaga
     4801 acgcatcgtt cattgcttca ctgtctgcca gaccaaacag gccttcgtac cactcgccgg
     4861 tgaagacgta tttttcacgt tcgcccggca ggtaacccca cacttcatca gcggtgaagg
     4921 agaggattgg tgccatccag cgaaccagcg cttctgcgat gtgatacagc gcagtctggc
     4981 agctacgacg cgccacgctg tccgctttgg cggtatactg acggtctttg atgatgtcga
     5041 ggtagaagga acccatctca acggagcaga agcgcatcag acgctgtacc acttcgtgga
     5101 aatcgtatgc ttcgtacgcc ttgaggatgt cttcctgtgc cgctttcgca caacctacgg
     5161 cccagcgatc cagtaccacc atctcttccg gtttcaccat atcttttgcc ggatcaaaac
     5221 cgttcaggtt tgccagcagg aagcgcgcgg tgttacggat acgacgatag ctgtcggcag
     5281 cacgtttcag gatctcgtca gaaacggcca tttcgccagt gtagtcggtt gatgccaccc
     5341 acagacgcag aatatccgca cccagtttgt tcatcacatc ctgcggcgaa acggtgttac
     5401 cgatggattt agacatcttg cgaccctgac catccacggt aaagccgtga gtcagtacct
     5461 gacgatatgg cgctttgccc ttcatcgcgg tagagatcat cagggaagac atgaaccagc
     5521 cacggtgttg gtcagaacct tccagataca tgtccgctgc gtgaccggca aattccggac
     5581 gcacatcaac aacggaagag tgggtagatc cggagtcaaa ccatacatcc agcgtatccg
     5641 gtactttcac atactggtca gcttcgtcgc cgaggatctc tttcgcatcg agatcccacc
     5701 acgcctgaat gccgtcaact tcaacgcgtt ttgccacttc ttccatcagt tcgagagtac
     5761 gcggatgcag ttcttctgtg tctttgtgca cgaacagtga catcggcacg ccccaggtac
     5821 gctgacgaga gatacaccag tcaggacggt tagcaaccat cgactcgata cgcgcctggc
     5881 cccagtcagg gatccactgc acgcctttga tctctttcag tgactgcgca cgcagacctt
     5941 tctgatccat gctgacgaac cactgcggcg tcgcgcggaa gatgatcggc gttttatgac
     6001 gccagcagca cggatagctg tgttgcattt tctcaacgtg caacagtgcg cctttttcct
     6061 gcaacaacgc aatgacaata tcgttcgctt tgaagacgtt aacgccatcc agagtcgggt
     6121 aagtacccag cagataagtg ccgtccgggc caaccgggtt agcggtttcc agaccatatt
     6181 tttgaccgat cacatagtcg tccggaccgt ggcctggcgc ggtatgaacg gcaccggtac
     6241 cggcatccag cgtaacgtgg tcgccgagaa ttgccggaac atcgaagtcc ataaacggat
     6301 gggtaaagcg caacagttcc agctcggcac ctttcaccgt gccaagaatg gtgtaatcgc
     6361 taacgccgat acgctgcatt acgctttcaa ccagatcttt cgcgaggatc acggcctgac
     6421 cgtcgatttg caccagcgca taatcaaaat caggtgcaat ggagattgcg cggttagcag
     6481 gcagcgtcca cggcgtggtg gtccagataa ccagcgaaat tgggccgtta acattgctta
     6541 cgccaaattt cgttttcagc gcatcctgat cgaccgcctg gaaagcgacg tcgatggacg
     6601 gagaagtttt gtcgtaatac tcaacttccg cttctgccag tgcagaacgg cagtcaacgc
     6661 accagtgcac cggcttcgcg cctttgtgca ggtgaccgtt gccgatgatt ttgcccagcg
     6721 cgcggatgat gttggcttca gttttgaagt ccatggtcag gtacgggtgc gaccagtcgc
     6781 ccagcacgcc cagacggata aagtctttgc gctgaccgtc aacctgggtc gcagcgtatt
     6841 cgcggcactt ggcgcggaac tcagcggcgg tgaatttctc ccccggctta ccgtattctt
     6901 gctctacttt cagttcgatt ggcagaccat gacagtccca gccaggcaca tacggcgagt
     6961 catatccaga aagccctttg gacttaatga taatgtcttt cagaatcttg ttaaccgagt
     7021 gaccaatatg aatgctgcca ttcgcataag gagggccatc atgcagaatg aaggtttttt
     7081 tgcctttttt agctgcacga atgatgccgt acaaatcatc atcagtccaa cgcgccagca
     7141 ttcccggttc gcgcttggcg agatcgccac gcatcgggaa ccctgtttcc ggcaaattca
     7201 gggttgattt atagtcactc atcagattct cgattccgta tttcggtttg attacataac
     7261 aggcttaagc cggttttgtt agcccaaaaa attcgcgggc ggttaattca tcacgcgcaa
     7321 tctgcgcttt cagttcgtcc agcgatgcaa atcgctgctc attgcgtatt tttttacgca
     7381 gcactacttg tatatggcga ccataaaggt ccattgcaac atctaacaaa tgcacttcca
     7441 gttgctggcg aataccggca accgttgggc gtgttccgat gtttgcaacg ccgggtaacg
     7501 gcttttcgcc aaggcccaac acttctaccg cataaacccc tttcaccggg gaaacctgac
     7561 ggcgtagcgg tacattcgcc gtcgggaaac ctatagtgcg ccctaattca tcaccgtgga
     7621 ctacacgccc ggagatagca aacgggtgcc ccagtaaact ttctgccaga gccagattgt
     7681 catccgcaag cgcctgacgc acggcggtgc tgctgatacg cacgccacct tcgcaaaaag
     7741 tttgcgtgct ggtgatatcg aagccgtatt ccatgcccgc tttctgtaat aacaagaaat
     7801 cgccttcacg accagcgcca aagcggaaat cgtcacctac ggcaagaaat tttacccgca
     7861 agtgcttcac cagaagatca ctgatgaagt tttgcgcggt taacgccgca aaacgcctgt
     7921 caaaacgcac gcacagcacg taatcaacac cacactctgc aagataacgc agtttttccc
     7981 gcagccgggt gagccgtgcc ggggctttat cagtagcaaa cagttccagt ggttgaggtt
     8041 caaaaagcat caccatcacc ggtaagttgc gcttgcgccc ttcttcctgc aagccctgta
     8101 acagcgcgcg atgaccgcga tgcacgccgt cgaaattacc aatagtcagc acacaccctt
     8161 cttgcggggc ctggctgaga ttatgtatgc cgcgtatcag cttcatgtct ggctcaaaac
     8221 agtgaaaatc gtccgagtat accttgtaca gcggtcaagg ttaaccggcg attgagtacc
     8281 gaaatcagaa agaagtggct tttcattgct atggcgcaaa tcatgggaag aaactgaccg
     8341 cctgctgcaa tttttctcgc ggaaaagctg tattcacacc ccgcaagctg gtagaatcct
     8401 gcgccatcac tacgtaacga gtgccggcac attaacggcg cttatttgca caaatccatt
     8461 gacaaaagaa ggctaaaagg gcatattcct cggcctttga attgtccata tagaacacat
     8521 ttgggagttg gaccttggct aatatcaaat cagctaagaa gcgcgccatt cagtctgaaa
     8581 aggctcgtaa gcacaacgca agccgtcgct ctatgatgcg tactttcatc aagaaagtat
     8641 acgcagctat cgaagctggc gacaaagctg ctgcacagaa agcatttaac gaaatgcaac
     8701 caatcgtgga ccgtcaggct gctaaaggtc tgatccacaa aaacaaagct gcacgtcata
     8761 aggctaacct gactgcacag atcaacaaac tggcttaatt gcctgttgtt gtcagctttg
     8821 taaaaaaacc cgctctggcg ggttttttta tatccatctg caggccaaaa ttcggcctgc
     8881 aaagtacaaa tgtttatttt gaagctggag taaacagcgc agaatagtct gtattgcaga
     8941 tacgctgtac tgcagggtgc tgaatcatcc tttcggcaaa aatcgcgtgg tactcttcca
     9001 tcacgttctc aacacggcct atctccacaa ccgagtcatc gttatagaaa tcattagcgt
     9061 aaagcgaagg tgcaacgaaa atagcgttat gcgtcgcccc aaaggctttc atcaacgcag
     9121 catcatcaaa ctcacccaaa atttcgacgt tcaagccctg ggagttaaac cagtttaata
     9181 atttacgccc caacattgag cgacgccccg gaataagtaa acgacgctct tcaagacagg
     9241 caggaaacgg cttttctggt agtgggttag tgcaccagaa actgacacca cattcgccaa
     9301 ttttcatgga aaacagccct tcctgctgag tggaatcgat cggacagtca gagatgatca
     9361 tatccagttt atgctgactc aactgctcta aaagcatctc gtgcgtcgat tcgaaacagc
     9421 gtagatggat ctgctcgtct tccacaactg cggcatccag aacactgctg accagacgtt
     9481 tggaaagtgc atctgccaca ccaacatcaa acaataagtt ggactctttg cgatagttga
     9541 cgatatccag catttcctgg cttaaggtga acattttatc ggcatagcga tagaccagtt
     9601 cccccagttc gctgggttcc agaccacgtc ctttacgctt aaatagtttc ccttgcaggc
     9661 gctcttccag cgcccggatc tgcccggtaa tggtttgtgg tgttaaataa agcgcctccg
     9721 ctgcgccaac cacagaacct tctttgtaga catgccagaa gtaatacaag tggttgtaat
     9781 tgatatgaga catgctcatt tctctccctg ataacaatga aaagggagcc gtttatggct
     9841 ccccagtaca tcgtcctgtc aaactgatgg acgtaaacga acgcgtaacc agctatatcc
     9901 aattaccgcc gaagaaattg aaccgacaag gatacctaat tttgcccagt taatcagttc
     9961 tggatctacg ctaccaaatg ccaggctggc aataaagata gacatagtaa aaccgatacc
    10021 gcacaggata ccaaccgcca taatttgctg gtaagtcgtt ccctctggca gatgtgccaa
    10081 tttcaaacgc agcgccaacc agcagaacag actaataccc agtggcttgc caatcagcaa
    10141 accagcgatg atccctaatg gcaggatgga ggtcaggcca tccagcgtga cgccttgcag
    10201 tgaaacgcca gcattagcaa atgcaaacag cggtaaaatc agatacgcca cccacgggtg
    10261 caacacatgc tccagtcgct tcgctggaga acgcccatgc ttctctttta acggaataaa
    10321 gaagccgaca atgacgcccg ccagggttgc gtgaaccccc gatttcaaca ccgctgtcca
    10381 cagcaccacg ccaaccagaa tataaacgcc cgtgcgacgt acaccacaca gattcaatac
    10441 cgcgagtacc gcaattgcta cagccgcgac gccaagagag gccatcgata agtcattagt
    10501 gtagaacaat gcgatgataa tgatggcccc aagatcgtcg ataatagcca gagccatcaa
    10561 aaagatcttc agcgctaacg gaacacgact tcccaacagc gccaacacac caagtgcaaa
    10621 ggcaatgtca gtcgccgccg ggattgccca gccttcgcga gtgataggat cggcatagtt
    10681 aaaagccaga tagagcaatg ccgggacaat catcccgccg attgcggcaa taacaggaaa
    10741 tgccgcctgg cgcagactgg ccagcgaacc ttgcatcagc tcgcgtttaa cttccagacc
    10801 aaccaacagg aaaaataccg ccatcagagc gtcattgatc catagcagca tgttcttgtt
    10861 gatctcaagt gtcccaaccc ggagctgaac cggcgtctca agaaagtcgt gataccatcc
    10921 actggttgca ccgctgttgg ccataatcat cgctaataca gcggcaataa tgagaataat
    10981 gcctcccgag gcatcactgc taaagaatcg atgcagatgt ttcacttttt atttctcttc
    11041 aggtgaatag atcgttttca cgattttacc ccgcacgaat catcgttaaa atcagattat
    11101 agtgtaggaa tagctcgctt tttacgatct attaaaagaa ttgcgccatc agcgagagat
    11161 gagtttttga tgcccgaaag cggcggctga tgaccttaaa ccccattaat catagtctaa
    11221 tgtcaagatt tctgaagaaa ttattcggaa aag
//
LOCUS       DADSZD010000140        11065 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.141, whole genome shotgun sequence.
ACCESSION   DADSZD010000140 DADSZD010000000
VERSION     DADSZD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.141"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            241..1383
                     /locus_tag="J1Z28_003433"
     CDS             241..1383
                     /locus_tag="J1Z28_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7718236.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     SEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRADLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAITDLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1390..2154)
                     /gene="glcC"
                     /locus_tag="J1Z28_003434"
     CDS             complement(1390..2154)
                     /gene="glcC"
                     /locus_tag="J1Z28_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBA7718237.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDASPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLATSENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMKQG"
     gene            2405..3904
                     /gene="glcD"
                     /locus_tag="J1Z28_003435"
     CDS             2405..3904
                     /gene="glcD"
                     /locus_tag="J1Z28_003435"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HBA7718238.1"
                     /translation="MSILYEERLDGALPEVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPIGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGADALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCEQVNDILLNAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3904..4956
                     /gene="glcE"
                     /locus_tag="J1Z28_003436"
     CDS             3904..4956
                     /gene="glcE"
                     /locus_tag="J1Z28_003436"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBA7718239.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRATLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMIDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSDGDGGFAPLPAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4967..6190
                     /gene="glcF"
                     /locus_tag="J1Z28_003437"
     CDS             4967..6190
                     /gene="glcF"
                     /locus_tag="J1Z28_003437"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HBA7718240.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMPANEVGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAGDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6195..6599
                     /locus_tag="J1Z28_003438"
     CDS             6195..6599
                     /locus_tag="J1Z28_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HBA7718241.1"
                     /translation="MKTKVILSQQMASAIISAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6621..8792
                     /gene="glcB"
                     /locus_tag="J1Z28_003439"
     CDS             6621..8792
                     /gene="glcB"
                     /locus_tag="J1Z28_003439"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBA7718242.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPDHVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDSAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFSRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKDQVQASLENMAKVVDQQNAGDPAYRPMVE
                     NFANSCAFKAACDLIFLGVKQPNGYTEPLLHAWRLREKENH"
     gene            9148..10830
                     /gene="glcA"
                     /locus_tag="J1Z28_003440"
     CDS             9148..10830
                     /gene="glcA"
                     /locus_tag="J1Z28_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HBA7718243.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFDIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLSLFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYRLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
BASE COUNT         2567 a         2750 c         3098 g         2650 t
ORIGIN      
        1 gcttttttgt ttttattgtg aatgatattt aatttgtgaa tatattaaga aggagccttg
       61 tccggtcgcc ttaaatcgac aaaaggcatt gtggcgcttc tgtgtagtct ctgaccttat
      121 cgtaatcatt aataaatact atttattctt tgtttgtatt cattgggttg attattaaga
      181 tgccgttcgt tgctgttgca acttgttttt caccgttaat tatttgttat ccacagagat
      241 atggaatgcg atctgctgat gattaaaatt gaaaaagtaa ttaataaaaa tgaccttaag
      301 gcatttatcg cttttccgtc atcactttat cccgatgatc caaactggat acccccttta
      361 ttcattgaac gcagcgagca tttgtctgcg aagaatccgg ggacggacca tatcatctgg
      421 caggcgtggg tggcaaaaaa agcggggcag atagttgggc gtattaccgc gcaaatcgat
      481 accttgcacc gtgagcgtta cggagaagat accggtcatt tcggaatgat tgatgccatt
      541 gatgatccgc aggtttttgc cgcgttgttt ggcgcagcgg aggcgtggtt gaagtcacaa
      601 ggtgcaagta agatcagcgg gcctttcagc ctgaatatca atcaggaaag cggattactg
      661 attgaaggtt ttgacacacc tccctgtgcg atgatgccac acggtaaacc gtggtatgcc
      721 gcgcatattg aacaactggg ttatcacaaa ggcattgatt tactggcgtg gtggatgcag
      781 cgagccgatc tcactttctc tccggcgcta aaaaaactga tggatcaggt gcgcaaaaaa
      841 gtgaccattc gctgcatcaa tcgccagcgg tttgccgaag agatgcagat cctgcgtgag
      901 attttcaatt ctggctggca gcacaactgg ggatttgtgc cgtttaccga acatgaattc
      961 gcgaccatgg gcgatcaact taaatatctg gtgccggacg atatgatcta catcgctgag
     1021 attgattctg caccctgcgc gtttattgtt ggcttgccga acatcaacga ggcgattacc
     1081 gatctgaacg gatcgctctt tcccttcggc tgggcaaaat tgctgtggcg tttgaaagtc
     1141 agcggtgtgc gaactgcgcg agtgccgctg atgggcgtgc gggacgagta tcagttcagc
     1201 cgcatcggcc cggtgattgc gctgttattg attgaagcct tacgcgatcc gtttgcccgc
     1261 cggaagatcg atgcgctgga aatgtcatgg atcctcgaaa ccaatacagg catgaataac
     1321 atgctggaac gcattggggc ggagccgtac aagcgttacc gattgtatga aaagcagatt
     1381 tgatggcagt cacccctgct tcatctccag cggcaccgcc gagcgaatca aatggtggcc
     1441 ttccagctcg atatcgtgga gattcttttt cacggtccgc acatgatcgc gtgctgcgcg
     1501 ctgggcaacg tgcggcagcc gctgcaacac cgcgttgtag atccgcgcat gctggcgatc
     1561 gatctgcttt ttttgctgtg gtcgatggta gagattattt acgctggcaa acaccgaatt
     1621 aaacatcagg tcggtcaatg attgcagcgt aaacaccagc acctgattgt gagaagcctg
     1681 acaaatggcg agatggaaag cgtgatccaa ctgcgcatgt tcgatcagcg aaatctcttt
     1741 gttgttctca ctggtggcga gcattttttc ataacagcgg gttatcacaa caaaatcagc
     1801 ctgggttccc agcgttgccg ccagccttgc cgattcgccc tccagtaatg cgcgaacgtc
     1861 gagcagatcg tacagcgttc gcggctgcgt actgaacaga tggatcagcg ggctggcgtc
     1921 ctgcacccga ttaagccgtg cgacacgaga atcgcgaccc tgcgccgttt caataatccc
     1981 gcgcccgcgc agcacggtca gcccttcacg cagtgcggag cgtgagaagc cgagcttttc
     2041 acacagtcga cgttccgagg gaagcggctg accgaccttc aacacgccgt cgataattaa
     2101 ccgttcgata ctctctgcaa ccacttcgca aatagggcga cgttcatctt tcattcccgg
     2161 acctcgtgca caggtaggac caatttttct gttcttaact cgcaaaacac gcacatcacg
     2221 taagtgatta tgttacatca atttaacatt aagttaacca aagcaaggtc acagagctgg
     2281 aaaaaaaatg gtctgaccgg tagctaaaga gatagacgaa aacgaaaagc ccgcttaata
     2341 actgttcaca gaagcagcgc gaaaaaatca gctgccacac aacacaacaa agcgaaccct
     2401 actcatgagc atcttgtacg aagagcgtct tgatggcgct ttacccgaag tcgaccgcac
     2461 atcggtactg atggcactgc gtgagcatgt ccctggactg gagatcctgc ataccgatga
     2521 ggagatcatt ccttacgagt gtgacgggtt gagcgcgtat cgcacgcgtc cattactggt
     2581 tgttctgcct aagcagatgg aacaggtgac ggcgattctg gctgtctgcc accgcctgcg
     2641 tgtgccggtg gtgacccgtg gcgcaggcac cgggctttct ggtggcgcgc tgccgctgga
     2701 aaaaggtgtg ttgttggtga tggcgcgctt taaagagatc ctcgacatta accccattgg
     2761 tcgccgcgcg cgcgtgcagc cgggcgtgcg taacctggcg atctcccagg ccgttgcgcc
     2821 gcataatctc tattacgcgc cggatccgtc ctcacaaatc gcctgttcga ttggcggcaa
     2881 tgtggcggaa aatgccggtg gcgttcactg cctgaaatat ggtctaaccg tacataacct
     2941 gctgaaaatt gaagtgcaaa cgctggatgg cgaggcgctg acgctgggag cggacgcgct
     3001 ggattcgcct ggttttgacc tgctggcgct gttcaccgga tcggaaggca tgcttggcgt
     3061 gaccaccgaa gtgacggtaa agctgctgcc gaagccgccc gtggcgcggg ttctgttagc
     3121 cagctttgac tcggtagaaa aagccggact tgcggttggt gacatcatcg ccaatggcat
     3181 tatccccggc gggctggaga tgatggataa cctgtcgatt cgcgcggcgg aagattttat
     3241 tcatgccggt tatcccgtcg atgccgaagc gattttgtta tgcgaactgg acggcgtgga
     3301 gtctgacgta caggaagact gcgagcaggt taacgacatc ttgttgaacg cgggtgcgac
     3361 tgacgtccgt ctggcacagg acgaagcaga gcgagtacgt ttctgggccg gtcgcaaaaa
     3421 tgcgttcccg gcggtaggac gtatctcccc ggattactac tgcatggatg gcaccatccc
     3481 gcgccgcgcc ctgcctggcg tactggaagg cattgcccgt ttatcgcagc aatatgattt
     3541 acgcgttgcc aacgtctttc atgccggaga cggcaacatg cacccgttaa tccttttcga
     3601 tgccaacgaa cccggtgaat ttgcccgcgc ggaagaactg ggcgggaaga tcctcgaact
     3661 ctgcgttgaa gttggcggca gcatcagtgg ggaacatggc atcgggcgcg aaaaaatcaa
     3721 tcaaatgtgc gcccagttca acagcgatga aatcacgacc ttccatgcgg tcaaggcggc
     3781 gtttgacccc gatggtttgc tgaaccctgg gaaaaacatt cccacgctac accgctgtgc
     3841 tgaatttggt gccatgcatg tgcatcacgg tcatttacct tttcctgaac tggagcgttt
     3901 ctgatgctac gcgagtgtga ttacagccag gcgctgctgg agcaggtgaa tcaggcgatt
     3961 agcgataaaa cgccgctggt gattcagggc agcaatagca aagccttttt aggtcgtcct
     4021 gtcaccgggc aaacgctgga tgtgcgttgt catcgcggca ttgttaatta cgacccgacc
     4081 gagctggtga taaccgctcg tgccggaacg ccgctggtgg cgattgaagc ggcgctggag
     4141 agcgccgggc aaatgctccc ctgcgagccg ccacattatg gtgaagaagc cacctggggc
     4201 gggatggtcg cctgcgggct ggcggggccg cgtcgcccgt ggagcggttc ggtccgcgat
     4261 tttgtcctcg gcacgcgcat cattaccggc actggaaaac atctgcgttt tggtggcgaa
     4321 gtgatgaaaa acgttgccgg atacgatctc tcacggttaa tggccggaag ctacggttgt
     4381 ctgggtgtgc tcactgaaat ctcgatgaaa gtgttaccgc gaccacgcgc caccctgagt
     4441 ctgcgtcggg aaatcagcct gcaagaagcc atgaatgaaa tcgcccagtg gcaactccag
     4501 ccattaccca ttagtggctt atgttacttc gacaatgcgt tgtggatccg ccttgagggc
     4561 ggcgaaggat cggtaaaagc agcgcgtgaa ctgctggggg gcgaagaggt tgcgggtcag
     4621 ttctggcagc aattgcgtga acaacaactg ccgttcttct cgttaccagg taccttatgg
     4681 cgcatttcct tacccagcga tgcgccgatg atagatttac ccggcgagca actgatcgac
     4741 tggggcgggg cgctacgctg gctgaaatcg acagccgatg acaatcaaat ccatcgcatc
     4801 gcccgcaacg ctggcggtca tgcgacccgc tttagtgacg gagatggtgg ttttgccccg
     4861 ctaccggctc ctttattccg ctatcaccag cagcttaaac agcagctcga cccttgcggc
     4921 gtgtttaacc ccggtcgcat gtacgcggaa ctttgaggat caggctatgc aaacccgatt
     4981 aacagaagag atgcggcaga acgcgcgcgc gctggaagcc gacagcatcc tgcgcgcctg
     5041 tgttcactgc ggattttgta ccgcaacctg cccaacctat cagcttctgg gcgatgaact
     5101 ggacgggccg cgcgggcgca tctatctgat taaacaggtg ctggaaggta acgaagtcac
     5161 gcttaaaacg caggagcatc tcgatcgctg tctcacttgc cgcaattgtg aaaccacctg
     5221 tccttctggt gtgcgctatc acaatttgct ggatatcggg cgtgatattg tcgagcagaa
     5281 agtgaaacgc ccactgccgg agcgaatgct gcgcgaagga ttgcgccagg tagtgccgcg
     5341 tccggcggtc ttccgtgcgc tgacgcaggt agggctggtg ctgcgaccgt ttttaccgga
     5401 acaggtcaga gcaaaactgc ctgctgaaac cgtgaaagct aaaccgcgtc caccgctgcg
     5461 ccataagcgt cgggttttaa tgctggaagg ctgcgcccag cctacgcttt cgcccaacac
     5521 caacgcggca actgcgcgag tgctggatcg tttggggatc agcgtcatgc cagctaacga
     5581 agtaggctgt tgtggcgcgg tggactatca tcttaatgcg caagagaaag ggttagcgcg
     5641 ggcgcgcaat aatattgatg cctggtggcc cgcgattgaa gcaggtgccg aggcaatttt
     5701 gcaaaccgcc agcggttgtg gcgcgttcgt caaagagtat gggcagatgc tgaaaaacga
     5761 tgcgttgtat gccgataaag cgcgtcaggt cagtgagctg gcgggcgatt tagtcgaact
     5821 tctgcgcgag gaaccgctgg aaaaactggc aattcgcggc gataaaaaac tggccttcca
     5881 ctgcccgtgt accctacaac atgcgcaaaa gctgaacggc gaagtggaaa aagtgttact
     5941 tcgtcttgga tttaccttaa cggacgttcc cgacagccat ctgtgctgcg gttcagcggg
     6001 aacatatgcg ttaacgcatc ccgatctggc gcgccagctg cgggataaca aaatgaatgc
     6061 gctggaaagc ggcaaaccgg aaatgatcgt caccgccaac attggttgcc agacgcatct
     6121 ggcgagcgcc ggtcgtacct ctgtgcgtca ctggattgaa attgtagaac aagcccttga
     6181 aaaggaataa caaaatgaaa actaaagtca ttcttagcca gcaaatggcg agtgcaatta
     6241 tttccgcagg tcaggaagag gcgcagaaaa ataactggtc tgtttccatt gctgttgccg
     6301 atgacggcgg tcatctgctg gcgttaagtc gcatggacga ttgcgcgccg attgcggctt
     6361 acatctccca ggagaaagcg cgtaccgccg cgctggggcg tcgtgaaact aagggctatg
     6421 aagagatggt gaacaacgga cgtaccgcgt tcgtgactgc gccgttactg acgtcgctgg
     6481 aaggtggcgt accggttgtt gtggatgggc aaattattgg tgccgtgggc gtttctggtt
     6541 taaccggagc acaggatgcg caggtcgcga aagcggcagc agcggtgttg gcgaaataag
     6601 cgaaaacgag gagataaaca atgagtcaaa ccataaccca gggccgttta cgcattgacg
     6661 ccaattttaa acgttttgtg gatgaagaag ttttgcctgg cgtggaactg gatgctgccg
     6721 cgttctggca caatgttgat gagatcgttc acgatctggc gccagagaat cgtcagttgc
     6781 tggcagagcg cgatcgcatt caggcggcgc ttgatgagtg gcatcgcagc aatccggggc
     6841 cggtaaaaga taaagcggcc tataaatctt tcctgcgtga actgggctac ctggtgccac
     6901 agcctgacca cgttacggtg gaaaccacgg gcatcgacag cgaaatcacc agtcaggcgg
     6961 ggccacagct ggtggttccg gcgatgaacg cccgctacgc gctgaacgcg gcgaacgctc
     7021 gctggggctc actgtacgat gcgttatacg gcagcgacat catcccgcag gaaggggcga
     7081 tggtcagcgg ctacgatccg cagcgtggtg agcaggttat cgcctgggtt cggcgtttcc
     7141 tcgatgaatc actaccgctg gaaaatggca gctatcagga tgtggtggcg tttaaggtgg
     7201 tcgataaaca attacgcatc cagttgaaaa atggtaaaga aaccacgtta cgtactccag
     7261 cacagtttgt cggttaccgt ggcgatactg ctgcgccgac ctgcattttg ctgaaaaata
     7321 acggcctgca tattgagcta caaatcgatg ccaacgggcg gattggcaaa gacgattcgg
     7381 cgcacatcaa cgatgttatc gtcgaagcgg ccatcagtac cattctcgac tgcgaagatt
     7441 cggttgcggc ggttgatgcg gaagataaaa tcctgctgta ccgcaacctg ctgggcctga
     7501 tgcaggggac tctgcaagag aaaatggaga agaacggtcg gcaaatcgtg cgtaaactga
     7561 atgacgatcg tcagtacact gccgccgatg gatctgaaat ttctctgcac ggacgctcgt
     7621 tgctgtttat ccgcaacgtg ggtcatttga tgaccattcc tgtgatttgg gacagcgaag
     7681 gcaatgaaat cccggaaggc attcttgatg gtgtcatgac tggcgcgatt gccctctatg
     7741 atttaaaagt gcagaaaaac tcgcgcactg gcagcgtcta tattgtgaaa ccgaaaatgc
     7801 acggtccgca ggaagtggcg ttcgccaaca aactgttttc ccgcgttgag acaatgctcg
     7861 gtatggcacc gaataccctg aaaatgggca ttatggatga agaacgccgg acctcgctga
     7921 acttgcgtag ctgtatcgct caggcgcgca accgcgtggc gttcatcaat accggtttcc
     7981 tcgaccgtac cggcgatgaa atgcattcgg tgatggaagc gggcccgatg ctgcgtaaaa
     8041 accagatgaa atcgacgccg tggatcaaag cctacgaacg taataacgtg ctttccggtc
     8101 tgttctgtgg gctacgtgga aaagcgcaaa tcggtaaagg catgtgggca atgccggacc
     8161 tgatggcaga catgtacagc cagaagggcg accaattgcg tgccggggca aacacggcct
     8221 gggttccgtc gccaactgcc gctacgctcc atgcgctgca ctaccaccaa accaacgtac
     8281 agagcgtgca agccaacatt gcccagaccg agttcaatgc tgaatttgaa ccgctgctgg
     8341 acgatctgct gactattccg gttgctgaaa acgctaactg gtcggcgcaa gagatccaac
     8401 aagagctgga taacaacgtg caggggattc tggggtacgt ggtgcgttgg gtggagcagg
     8461 ggattggttg ttcaaaagtg ccggatattc acaacgtggc gttgatggaa gaccgcgcaa
     8521 cgctgcgtat ttccagccag catatcgcca actggttacg tcacggtatt ctgaccaaag
     8581 atcaggtgca ggcgtcgctg gagaatatgg cgaaagtggt tgatcagcaa aacgctggcg
     8641 atccggctta tcgtccaatg gtggagaatt tcgctaactc gtgtgctttt aaagctgcct
     8701 gcgatttgat cttcctcggc gtgaaacagc caaacggcta taccgaaccg ttattacacg
     8761 catggcgttt acgcgaaaaa gaaaatcatt aatagcgtca aaaaaagccc cggcaagatt
     8821 tattgtcggg gctatgatag taactgcggt agtacattta tcccggaaat aaccggagcg
     8881 attatcaggg gttaacaata ttaaataccc gtagcgcatc actagcggga ttaaaacaac
     8941 aatatggaca tcttaacgat actctgcgcc tgcgtgtgcg gaacagaaac ccttacccga
     9001 tatacttgtt atgccagagg tagcaattgc gttagcggcg tactgttgct gtaaagtata
     9061 acgcctatat tttacagttc agtcctcttt tgcttattac aaccttaagt atatcaagca
     9121 tataaagata ataagagact gaacaatatg gttacctgga cccaaatgta tatgccgatg
     9181 ggaggactgg ggctatccgc tctggtcgcc ctgatcccaa taatattctt cttcgttgca
     9241 ctcgcggtat tacgtctgaa aggacatgtc gctggagcaa taacccttat attatctatt
     9301 ctgattgcga tattcgcctt taaaatgccg attgatatgg catttgctgc tgcgggctat
     9361 ggctttattt atggtttatg gcctattgcg tggattattg tcgcggcggt attcctgtat
     9421 aaattaaccg ttgccagcgg ccagttcgat attatccgca gctcggttat ctccatcacc
     9481 gacgatcagc gtttgcaggt gttactgatt ggtttctcct ttggtgcgct gctggaaggg
     9541 gcggctggct ttggcgcacc ggtggcgatt accggtgctc tactggtggg cctgggcttc
     9601 aaaccgctat acgcggcggg gctgtgtctg attgccaaca ctgcgccggt ggcgtttggt
     9661 gcgttgggtg tgccaattct ggtcgccggt caggtaacgg gaatcgatcc gttcgacatt
     9721 ggcgcaatgg cgggacgtca gttaccgttc ctgtcggttc ttgtgccgtt ctggctggta
     9781 gcaatgatgg acggctggaa aggggtgaaa gagacgtggc cagcggcgct ggttgcaggg
     9841 ggaagcttcg ctgtcactca gttctttacc tctaactata ttggtccgga actgccggat
     9901 attacttcgg cgctggtgag tatcgtctca ctttctttat ttcttaaagt ctggcggcca
     9961 aaaaataccg aaacggcaat cagtatggga caatccgcag gtgcgatggt ggtgaataaa
    10021 ccgtcttctg gcggtcccgt gccttcagaa tatcgtctgg ggcaaatcat tcgtgcgtgg
    10081 tcaccgtttt taatcttaac ggtgttggtc accatctgga ccatgaagcc gtttaaagcg
    10141 ttatttgctc cgggcggcgc gttttattca ctggtgatta atttccagat ccctcatttg
    10201 catcaacaag tcttgaaagc ggcccccatt gtcgcccagc cgacgccaat ggacgcggtg
    10261 tttaaattcg accccctctc cgctggcggc accgctattt ttattgcggc gattatctcg
    10321 atcttcatcc tcggcgtggg gatcaagaaa ggtattggtg tctttgccga aacgctaatt
    10381 agtctgaagt ggccgatatt gtcgattggc atggtgctgg cgttcgcctt cgtgaccaac
    10441 tattccggta tgtccaccac gctggcgctg gtactggctg gtacaggcgt gatgttccca
    10501 ttcttctcac cgttcctcgg ctggctgggc gtattcctta ccggctcgga cacttcttca
    10561 aacgccctgt ttggttcact gcaatcgacc acggcgcagc aaatcaacgt ctctgacacc
    10621 ctgctggtgg cagcaaacac cagcggcggc gtaactggca agatgatctc cccgcaatct
    10681 atcgccgtgg cctgcgccgc gacgggcatg gtggggcgag aatctgaact gttccgctac
    10741 accgtgaagc acagtctgat ttttgccagc gttatcggca ttatcacgct gttgcaggcg
    10801 tatgtgttta ccgggatgtt agtttcgtaa gtcaataatg ccggatgatc gttaatctca
    10861 tccggcttcg tttacattat tctgtcttat taatggaaaa attatttccc gctttattat
    10921 ccatgcggca ataatgtttt gcgttcagat aacatgctaa ataattaaga gcgtgagaac
    10981 atctattttt tatcacgcaa aacaagtctg tattaattat gttcgcttga tgagcaaact
    11041 ccgttctatc tgttgctttt gcaat
//
LOCUS       DADSZD010000141        11005 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.142, whole genome shotgun sequence.
ACCESSION   DADSZD010000141 DADSZD010000000
VERSION     DADSZD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.142"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..158
                     /locus_tag="J1Z28_003441"
     CDS             <1..158
                     /locus_tag="J1Z28_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBA7718244.1"
                     /translation="ITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQ
                     EELKALV"
     gene            complement(278..457)
                     /locus_tag="J1Z28_003442"
     CDS             complement(278..457)
                     /locus_tag="J1Z28_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBA7718245.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            531..1892
                     /gene="pabB"
                     /locus_tag="J1Z28_003443"
     CDS             531..1892
                     /gene="pabB"
                     /locus_tag="J1Z28_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBA7718246.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKCTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAQQDIVLPDMAVGIYDWALVVDHQRQTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGTILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDLQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            1896..2474
                     /locus_tag="J1Z28_003444"
     CDS             1896..2474
                     /locus_tag="J1Z28_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBA7718247.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2658..4022
                     /locus_tag="J1Z28_003445"
     CDS             2658..4022
                     /locus_tag="J1Z28_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine dehydratase 1"
                     /protein_id="HBA7718248.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4153..5751
                     /locus_tag="J1Z28_003446"
     CDS             4153..5751
                     /locus_tag="J1Z28_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBA7718249.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGNGLVDSLPQPENNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5755..7311)
                     /gene="yoaE"
                     /locus_tag="J1Z28_003447"
     CDS             complement(5755..7311)
                     /gene="yoaE"
                     /locus_tag="J1Z28_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBA7718250.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7774..8745
                     /gene="manX"
                     /locus_tag="J1Z28_003448"
     CDS             7774..8745
                     /gene="manX"
                     /locus_tag="J1Z28_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBA7718251.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPIEKAAPAPAAAAPKVAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8808..9608
                     /locus_tag="J1Z28_003449"
     CDS             8808..9608
                     /locus_tag="J1Z28_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBA7718252.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAAIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9621..10472
                     /locus_tag="J1Z28_003450"
     CDS             9621..10472
                     /locus_tag="J1Z28_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBA7718253.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10527..10985
                     /locus_tag="J1Z28_003451"
     CDS             10527..10985
                     /locus_tag="J1Z28_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBA7718254.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVIQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2683 a         2799 c         2772 g         2751 t
ORIGIN      
        1 atattacggc gcgagctgac caggaaggct ggaaccctgg tttcacggaa aaaatggttg
       61 gatgggcaaa aaaaatggag tctggtgaac gctctgtgat taaaaatcct gaatactttt
      121 caacatatat gcaggaagaa ctaaaagcac tggtttgagt taaatttaca tcagcataaa
      181 tgggtcagga cgcttttaat cacatataaa aagcgtcctt tttctcacaa tcaaataatc
      241 atcggcttct ataacgaaag cgatacggat tagtgtgtta ctcgtcttca tcctcaaaac
      301 gcgccacgat tcgctcgccg gaatggttgg cacgcagctc ttctgccacc agcgcgattg
      361 cctggccgct gctcatccct tgtgccatca gttcctggat ccgctcgacg gctttttgct
      421 gctgttcatg ggtgagtgaa ggtaaacctg caaacattgt taactcctgc taaattgttg
      481 gcgctaatta tttcatgcta cccggcacat agccagtaga gtcaggactg atgaagacgt
      541 tatctcccgc tgtgattact ttaccctggc gtcaggacgc cgctgaattt tatttctccc
      601 gcttaagcca cctgccgtgg gcgatgcttt tacactccgg ctatgccgat catccgtata
      661 gccgctttga tattgtggtc gccgagccga tttgcacttt aaccactttc ggtaaagaaa
      721 ccgttgttag tgaaagcgaa aaatgcacaa cgaccactga tgacccgcta caggtgctcc
      781 agcaggtgct ggatcgcgca gacattcgcc caacgcataa cgaagatttg ccatttcagg
      841 gcggcgcgct ggggttgttt ggctacgatc tgggccgccg ttttgagtca ctgccagaaa
      901 ttgcgcagca agatatcgtt ctgccggata tggcagtggg tatctacgac tgggcgctgg
      961 ttgttgacca ccagcgtcaa acagtttctt tgctgagtca taatgatgtc aatgcccgtc
     1021 gggcctggct ggaaagccag caattctcgc cgcaggaaga tttcacgctc acttccgact
     1081 ggcaatccaa tatgacccgc gagcagtacg gcgaaaaatt tcgccaggta caggaatatc
     1141 tgcacagcgg tgattgctat caggtgaatc tcgcccagcg ttttcatgcg acctattctg
     1201 gcgatgaatg gcaggcattc cttcagctta atcaggccaa tcgcgcgccg tttagcgctt
     1261 ttttacgcct tgaacaaggc acgattttaa gcctttcgcc agagcggttt attctttgtg
     1321 ataatagtga aattcagacc cgcccgatta aaggcacgct accacgcctg cccgatcttc
     1381 aggaagatag caaacaagca gaaaaactgg cgaactcagc gaaagatcgt gccgaaaatc
     1441 tgatgattgt cgatttaatg cgtaatgata tcggtcgtgt tgccgtagca ggttcggtaa
     1501 aagtaccaga gctgttcgtg gtggaaccct tccctgccgt gcatcatctg gtcagcacca
     1561 taacggcgca actaccagaa cagttacacg ccagcgatct gctgcgcgcg gcttttcctg
     1621 gtggctcaat aaccggggct ccgaaagtac gggctatgga aattatcgac gaactggaac
     1681 cgcagcgacg caatgcctgg tgcggcagca ttggctattt gagcttttgc ggcaacatgg
     1741 acaccagcat tactatccgc acgctgactg ccattaacgg acaaatttac tgctctgcgg
     1801 gcggtggaat tgttgccgat agccaggaag aagcggaata tcaggaaact tttgataaag
     1861 ttaataagat attacgccaa ctggagaagt aagacgtgga ataccgtagc ctgacgcttg
     1921 atgatttttt atcgcgcttt caacttttgc gcccgcaaat taaccgggaa accttaaatc
     1981 atcgtcaggc tgccgtgtta atccccatcg tccgccgacc gcaaccgggg ttgttgctga
     2041 ctcagcgttc gattcatctg cgtaaacacg ctggacaagt ggcattccct ggaggcgcag
     2101 tcgatgacac ggacgcatca gttatcgccg ctgcgctgcg cgaagctgaa gaagaggtcg
     2161 ctataccgcc ttccgccgtt gaggttatcg gcgtgctgcc gcccgtcgat agcgtcactg
     2221 gctatcaggt aaccccagtg gtcggtatta tcccgcccga tctgccgtat cgcgccagtg
     2281 aagatgaagt ctccgccgtc tttgaaatgc cgctcgccca ggcattacat ctgggtcgtt
     2341 atcatccttt agatatctac cgccgtggcg attcacatcg ggtatggctg tcctggtacg
     2401 aacagtattt tgtatgggga atgaccgcag gcataattcg tgagctggcg ctgcaaattg
     2461 gtgtgaaacc ctgactatac ttatctttac atctacaaaa cactacttga gacaatcatc
     2521 gcaatattag ttaaatcgcg gtttttgatt agtttaattc atgtgaatag ttaagccagt
     2581 cgccgcgttc cctcttacac tatgcgctgt tattcgttcg ttactggaag tccagtcacc
     2641 ttgtcaggag tattatcgtg attagtctat tcgacatgtt taaggtgggg attggtccct
     2701 catcttccca taccgtaggg cctatgaagg cgggtaaaca gttcgtcgat gatctggtcg
     2761 aaaaaggctt actggatagc gttactcgcg ttgccgtgga cgtttatggt tcactgtcgc
     2821 tgacgggtaa aggccaccac accgatatcg ccattattat gggtcttgca ggtaacgaac
     2881 ctgccaccgt ggatatcgac agtattcccg gttttattcg cgacgtagaa gagcgcgaac
     2941 gtctgctgct ggcacaggga cggcatgaag tggatttccc gcgcgacaac gggatgcgtt
     3001 ttcataacgg taacctgccg ctgcatgaaa acggtatgca aatccacgct tataatggcg
     3061 atgaagtcgt ctacagcaaa acttattatt ccatcggcgg cggttttatc gtcgatgaag
     3121 aacactttgg tcaggatgcc gccaacgaag tgagcgtgcc gtatccgttc aaatctgcca
     3181 ccgaactgct cgcgtactgt aatgaaaccg gctattcgct ttctggtctc gctatgcaga
     3241 acgaactagc gctgcacagc aagaaagaga tcgacgagta tttcgcgcat gtctggcaaa
     3301 ccatgcaggc atgtatcgat cgcgggatga ataccgaagg cgtactgcca ggcccgttgc
     3361 gcgtgccacg tcgtgcgtcg gccctgcgcc ggatgctggt ttccagcgat aaactgtcta
     3421 acgatccgat gaatgtcatt gactgggtaa acatgtttgc gctggcagtt aacgaagaaa
     3481 acgccgccgg tggccgtgtg gtaactgcgc caactaacgg tgcctgcggt atcgttccgg
     3541 cagtgctggc ttactatgac cactttattg aatcagttag cccagacatc tatacccgtt
     3601 actttatggc agcgggcgcg attggtgcac tgtataaaat gaacgcgtct atttccggtg
     3661 cggaagtcgg ttgccagggc gaagtaggtg ttgcctgttc aatggctgct gcgggccttg
     3721 ccgagctgtt gggcggtagc ccggaacagg tttgtgtggc agcggaaatt ggcatggaac
     3781 acaacctcgg tctcacctgc gaccccgttg cagggcaagt tcaggtgccg tgcattgaac
     3841 gtaatgccat tgcctctgtg aaggcgatta acgccgcacg gatggctctg cgccgcacca
     3901 gtgcaccgcg cgtctcgctg gataaggtca tcgaaacgat gtacgaaacc ggtaaggaca
     3961 tgaacgctaa ataccgcgaa acctcacgcg gtggtctggc aatcaaagtc cagtgtgact
     4021 aatactttat cctcgcccat ctgcaacgga tgggcgaatt tattcccgct tcctcgtctg
     4081 ctgtaatttt ccccactaca cttccactgt tgcgtcaggc gtttgtcgcc atacgcttac
     4141 agggtggccc gcatgcaaaa agcacaacgg atcattaaaa cctatcgccg taatcgaatg
     4201 attgtttgta cgatttgcgc actcgttacg ctcgcttcga ccctgagcgt gcgatttatt
     4261 tcacagcgta acttaaatca acaacgggta gtacaattcg ccaatcacgc tgtagaggaa
     4321 ttagataaag tactgcttcc cctacaggct ggtagcgaag tcttgcttcc gctgattggt
     4381 ctgccctgct ctgtcgccca tttgccatta cgtaaacagg cggcaaaact ccaaactgtg
     4441 cgatccattg gcctggtgca agacggcaca ctttattgct ccagcatttt tggttatcgc
     4501 aatgtgcccg tcgtggacat tctggctgaa cttcctgcac cgcaaccact tttacgactg
     4561 acgaccgacc gtgccctgat aaaaggcagt ccggttttga ttcaatggac gcctgcagcg
     4621 ggcagtagca atgctggggt catggagatg attaacatcg acttactgac ggcaatgctc
     4681 cttgaaccac aactgccgca aatcagtagc gccagcctga cggtggacag tcggcatttg
     4741 ctctatggta atgggctggt agattccctt ccgcaacctg aaaacaatga aaactatcag
     4801 gtttcttcgc aacgctttcc ttttaccatt aacgttaatg gcccgggggc tacggcgctg
     4861 gcatggcact atcttccaac acaattaccg ctggcggtgc tgctaagttt actggtgggc
     4921 tacatcgcct ggctggcgac cgcttaccga atgagctttt cccgcgagat caatctgggc
     4981 ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg cagccagcaa
     5041 tgtattggtg tagagatttt gctgcgctgg aacaatccac gtcagggctg gatttcaccg
     5101 gatgtgttta ttcctatcgc ggaagaacat catttaattg taccactgac ccgctatgtg
     5161 atggcagaaa ccattcgtca gcgccatgtt ttcccaatga gtagtcagtt tcatgttggc
     5221 attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct caatcagtac
     5281 tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg cgatgcctta
     5341 ctggatgttg attaccggat tgcccgcgag ctgcatcgta aaaacgtcaa actggcgatt
     5401 gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg tcctgacgtt
     5461 ctgaaaattg ataagtcatt taccgcagct ataggttctg atgcggttaa ctcgacggtg
     5521 accgatatca tcatcgcact ggggcaaaga ctgaatattg aactggtggc ggagggcgta
     5581 gaaacgcaag aacaggcgaa gtatttgcgc agtcatggcg tgcatatttt gcaagggtat
     5641 ttgtacgcac agccgatgcc gctacgtgat tttcccaaat ggctggcggg cagccaaccg
     5701 ccgcccgccc ggcataatgg acatatcaca cccattatgc cgttacgtta acgcttactc
     5761 atcttcatcg tgcgccggtt gctctttaac aatgcgaacc agatcaacac gataatcatt
     5821 ggcttcaatg atggtgatat gcagtggccc tacatcaatc acatcgccca cacggggaat
     5881 gtgaccattt gccgagatca cgaggcccgc gaccgtcgcg atatcatcgt catcggcaag
     5941 gtgctcaaca tcaagcgcct gctgcaaggc atgcaaatct gtaccgcctt ttaccagcca
     6001 gccgtcacca tcggtaatga tttccggcgt ttcgtcagcg tccgggaatt cacccgcaat
     6061 ggcttccagc acatccagcg gcgtgaccag accttgtacc acaccaaact cgttggtcac
     6121 gataacaaag ctcccgcgag cacgacgcag cacgcccagc aggttgatcg gatcgagggt
     6181 ttccgggacg ataatcgccg gagacgccga agctatcgcc gccacatcaa ctccctcttc
     6241 cagcgccacc agcagttctt tagcacgtac gatgccgata atttcatcca gttcaccgcg
     6301 acataccggg aacaaactgt gcggtgagga gagcagttgc tcgcggattt catcgacccc
     6361 gagattagca tcaacccagc ttatttcacc gcgcggcgtc atgatcccgc gcagagaacg
     6421 cgacgccagc gtcagtacgc cgttaatcat gtaacgttct tcttcggcaa acgcaccttc
     6481 cgggatcggc atcggcatcg ggttatcggc atcgtgctga acattggcct gacgtttccc
     6541 gcccatcaaa cgcaggatgg catcggcagt acgcgctcgc agcggcaaag tcgactggtg
     6601 acgaataaag ttgcgacgcg caatctggtt aaacacttcg atgatgatcg agaagccaat
     6661 cgcggcatac aggtaacctt tcggaatgtg gaaaccgaaa ccttctgcca ccagactcag
     6721 accaatcatt aacaggaagc ttagacagag caccaccacc gttggatgct ggttaacaaa
     6781 tcgcgtcagc ggtttcgatg ccagcagcat aaccgccatc gcaatcacta ccgccgccat
     6841 catcaccggc aaatggttaa ccatccctac tgcagtaatt accgcatcca acgagaagac
     6901 ggcgtcaagg atgacaatct gtgtgacgac cacccagaaa ctggcgtagc ctttaccgtg
     6961 gccggaatca tgatcgcggt tttccagccg ttcatgcagt tcagttgttg ctttgaacag
     7021 caagaatatg cccccgaaca acataatcag gtcgcgtccg gagaaggaga aatccatgac
     7081 ggtaaatagc ggtttggtca gcgtgaccat ccatgaaatc agcgacagca gccccagacg
     7141 cataatcagc gccagtgata atcccagcaa acgcgcttta tcgcgttgtt ttggcggcag
     7201 tttgtcagca agaatggcga tgaagaccag gttatcgata cccagcacaa tttcgagaac
     7261 aacaagcgtg agtagccccg cccaaatcga ggggtccatt aagaattcca tgacaagctc
     7321 ctgcttaagg aatagctatt cgacgccaga aataatgcag gcgtaacgac aaaatgcaaa
     7381 cgaaaggtgc ggcataaagt gccagaaagg caggcgttaa aaggcctgat gctgaaatga
     7441 cgtcggtgac gatccatact gcgggctact gccctatact ccatggttgt taaacaggag
     7501 ttaaacatat cagagacgcc tctgatttgg caaagattta ccttcctttg caaacgaatg
     7561 tgacaaggat attttacctt tcgaaatttc tgctaatcga aagttaaatt acggatcttc
     7621 atcacataaa ataatttttt cgatatctaa aataaatcgc gaaacgcagg ggtttttggt
     7681 tgtagccctt atctgaatcg attcgattgt ggacgacgat tcaaaaatac atctggcacg
     7741 ttgaggtgtt aacgataata aaggaggtag caagtgacca ttgctattgt tataggcaca
     7801 catggttggg ctgcagagca gttgcttaaa acggcagaaa tgctgttagg cgagcaggaa
     7861 aacgtcggct ggatcgattt cgttccaggt gaaaatgccg aaacgctgat tgaaaagtac
     7921 aacgctcagt tggcaaaact cgacaccact aaaggcgtgc tgtttctcgt tgatacatgg
     7981 ggaggcagcc cattcaatgc tgccagccgc attgtcgtcg acaaagagca ttatgaagtc
     8041 attgcaggcg ttaacattcc aatgctcgtg gaaacgttaa tggcccgaga tgatgaccct
     8101 agctttgatg aactggtggc actggcagta gaaacaggcc gtgaaggcgt gaaagcactg
     8161 aaagccaaac cgattgaaaa agccgcgcca gcacccgctg ccgcagcacc caaagtggct
     8221 ccaactccgg caaaaccaat gggaccgaac gactacatgg ttattggcct tgcgcgtatc
     8281 gacgaccgtc tgattcacgg tcaggtcgcc acccgctgga ccaaagaaac caatgtctcc
     8341 cgtattattg ttgttagtga tgaagtggct gcggataccg ttcgtaagac actgctcacc
     8401 caggttgcac ctccgggcgt aacagcacac gtagttgatg ttgccaaaat gattcgcgtc
     8461 tacaacaacc cgaaatatgc tggcgaacgc gtaatgctgt tatttaccaa cccaacagat
     8521 gtagagcgtc tcgttgaagg cggcgtgaaa atcacctctg ttaacgtcgg tggtatggca
     8581 ttccgtcagg gtaaaaccca ggtgaataac gcggtttcgg ttgatgaaaa agatatcgag
     8641 gcgttcaaga aactgaatgc gcgcggtatt gagctggaag tccgtaaggt ttccaccgat
     8701 ccgaaactga aaatgatgga tctgatcagc aaaatcgata agtaacgtat tgtgttgatt
     8761 atcattcagt tttcacactt tagtcttacg taaacaggag aagtacaatg gagattacca
     8821 ctcttcaaat tgtgctggta tttatcgtag cctgtatcgc aggtatggga tcaatcctcg
     8881 atgaatttca gtttcaccgt ccgctaatcg cgtgtaccct ggtgggtatc gttcttgggg
     8941 atatgaaaac cggtattatt atcggtggta cgctggaaat gatcgcgctg ggctggatga
     9001 acatcggtgc tgccgttgcg cctgacgccg ccctggcttc tatcatttct accattctgg
     9061 ttatcgcagg tcatcagagc attggtgcag gtatcgcact ggcaatccct ctggccgctg
     9121 caggccaggt actgaccatc atcgttcgta ctattaccgt tgctttccag cacgctgcgg
     9181 ataaggcagc tgataacggc aacctgacag caatttcctg gatccacgtt tcttctctgt
     9241 tcctgcaagc aatgcgtgtg gctattccgg ccgccatcgt tgcgctgtct gttggtacca
     9301 gcgaagtaca gaacatgctg aatgcgattc cggaagtggt gaccaatggt ctgaatatcg
     9361 ccggtggcat gatcgtggtg gttggttatg cgatggttat caacatgatg cgtgctggct
     9421 acctgatgcc gttcttctac ctcggcttcg taaccgcagc attcaccaac tttaacctgg
     9481 ttgctctggg tgtgattggt actgttatgg cagtgctcta catccaactt agcccgaaat
     9541 acaaccgcgt agccggtgcg cctgctcagg ctgctggtaa caacgatctc gataacgaac
     9601 tggactaaca ggtgagcgaa atggttgata caactcaaac taccaccgag aaaaaactca
     9661 ctcaaagtga tattcgtggc gtcttcctgc gttctaacct ctttcagggt tcatggaact
     9721 tcgaacgtat gcaggcactg ggtttctgct tctctatggt accggcaatt cgtcgcctct
     9781 accctgagaa caacgaagct cgtaaacaag ctattcgccg tcacctggag ttctttaaca
     9841 cccagccatt cgtggctgcg ccgattctcg gcgtaaccct ggcactggaa gaacagcgtg
     9901 ctaatggcgc agagatcgat gacggtgcta tcaacggtat caaagtcggt ttgatggggc
     9961 cgctggctgg tgtaggcgac ccgatcttct ggggaacggt acgtccggta tttgcagcgc
    10021 tgggggctgg tatcgcgatg agcggcagcc tgttaggtcc gctgctgttc ttcatcctgt
    10081 ttaacctggt gcgtctggca acccgttact acggcgtagc gtatggttac tccaaaggta
    10141 tcgatatcgt taaagatatg ggtggtggct tcctgcaaaa actgacggaa ggggcgtcta
    10201 tcctcggcct gtttgtcatg ggggcattag ttaataagtg gacgcatgtc aacatcccgc
    10261 tggttgtctc tcgcattact gaccagacgg gcaaagaaca cgttaccact gtccagacta
    10321 ttctggacca gttaatgcca ggcctggtac cactgctgct gacctttgct tgtatgtggc
    10381 tactgcgcaa aaaagttaac ccgctgtgga tcatcgttgg cttcttcgtc atcggtatcg
    10441 ctggttacgc ttgcggcctg ctgggactgt aagactgttg tacactaccg gggccttttg
    10501 gccccgtttt tttatctgga ggattaatga caatcacgga cctggtactg attcttttca
    10561 tcgccgcact tctggccttc gcgatctacg atcagttcat catgccccgc cgtaacggcc
    10621 ccaccctgct ggcaattcct ttgcttcggc gtggtcgcat cgatagcgtt atcttcgtcg
    10681 gattgattgt cattcttatc tataacaacg tcacgaatca tggcgcgcta ataacaacat
    10741 ggttattaag tgcactggct ctgatgggtt tttatatatt ctggatccgc gttccgaaga
    10801 tcatctttaa acaaaaaggt tttttcttcg ccaatgtctg gattgaatat agccgaatca
    10861 aagcgatgaa cttgtcggaa gatggcgtgt tggtgataca attagaacag cgtcggctgt
    10921 taatccgcgt tcgaaatatc gacgatttgg aaaaaattta taagcttctc gtttcaactc
    10981 aataagttat gaatttagcc aaagc
//
LOCUS       DADSZD010000142        10934 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.143, whole genome shotgun sequence.
ACCESSION   DADSZD010000142 DADSZD010000000
VERSION     DADSZD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.143"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            68..829
                     /locus_tag="J1Z28_003452"
     CDS             68..829
                     /locus_tag="J1Z28_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7718255.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKESISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            966..2303
                     /gene="adiC"
                     /locus_tag="J1Z28_003453"
     CDS             966..2303
                     /gene="adiC"
                     /locus_tag="J1Z28_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBA7718256.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2407..4050
                     /gene="eptA"
                     /locus_tag="J1Z28_003454"
     CDS             2407..4050
                     /gene="eptA"
                     /locus_tag="J1Z28_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBA7718257.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKIKPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4047..4715
                     /gene="basR"
                     /locus_tag="J1Z28_003455"
     CDS             4047..4715
                     /gene="basR"
                     /locus_tag="J1Z28_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBA7718258.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4716..5816
                     /gene="pmrB"
                     /locus_tag="J1Z28_003456"
     CDS             4716..5816
                     /gene="pmrB"
                     /locus_tag="J1Z28_003456"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBA7718259.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5792..5881)
                     /gene="pmrR"
                     /locus_tag="J1Z28_003457"
     CDS             complement(5792..5881)
                     /gene="pmrR"
                     /locus_tag="J1Z28_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBA7718260.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5993..7495)
                     /gene="proP"
                     /locus_tag="J1Z28_003458"
     CDS             complement(5993..7495)
                     /gene="proP"
                     /locus_tag="J1Z28_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBA7718261.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7759..8637)
                     /locus_tag="J1Z28_003459"
     CDS             complement(7759..8637)
                     /locus_tag="J1Z28_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718262.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGEHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8634..10862)
                     /gene="crfC"
                     /locus_tag="J1Z28_003460"
     CDS             complement(8634..10862)
                     /gene="crfC"
                     /locus_tag="J1Z28_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBA7718263.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQHWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     NSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQAATDEQSTRLGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESMALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLCDDIQTLFTAE
                     RY"
BASE COUNT         2753 a         2877 c         2662 g         2642 t
ORIGIN      
        1 aaccatttcc cggtatcgct tcgctttagc gagaactggt cttttattcg cactcaggag
       61 tacatgtatg aggatttgca gcgaccaacc ttgtattgtt ttattgactg aaaaagatgt
      121 ctggataagg gtgaatggga aagaatctat tagccttaaa gctaaccaca tggcgttatt
      181 aaattgtgaa aataatatta tcgacgtctc ctctcttaat aacactttgg ttgctcatat
      241 tagtcacgac atcatcaaag attacctccg gtttctgaat aaagatctct cgcaaatacc
      301 agtatggcaa cgtagcgcta cgcccatcct caccctgcca tgcctgacgc cagacgtctt
      361 tcgcgttgcc gcgcaacata gcatgatgcc cgctgaaact gagtcagaaa aggaacgaac
      421 acgtgcatta ttattcactg tgctatcccg ttttctcgac agtaaaaaat tcctttcact
      481 aatgatgtat atgttacgta attgtgtaag tgacagcgtt tatcaaatta ttgaaagcga
      541 tatccacaaa gactggaatc ttagtatggt agccagttgt ttatgtctta gcccaagtct
      601 gttaaagaaa aagctgaaaa gcgaaaacac cagttatagc caaataatca ccacctgccg
      661 catgcgctat gccgtaaatg aattaatgat ggacggtaaa aatatctctc aggtatcaca
      721 gtcctgcggc tacaacagta cgtcgtactt tatttctgtc tttaaagact tctacggtat
      781 gacgccgctg cattatgtta gtcagcacag agaacgcact gtcgcctgat ttttaacctt
      841 aacgaagagc tatattaata acggcatcag cgataacccg gccgataata attcaacgat
      901 cgaatgcagg cgtatgatat gacgtaattt attgtcacga agctcgcctt cgcaggagtt
      961 taattatgtc ttcggatgct gatgctcaca aagtgggctt aatccccgtc accctgatgg
     1021 tgtcggggaa tattatgggg tcaggtgttt ttctgttacc tgcaaacctg gcctccactg
     1081 gcgggattgc catttatgga tggttggtga cgattatcgg tgcgctgggg ctctcgatgg
     1141 tatacgccaa aatgtcgttc ctcgacccaa gtcctggtgg ttcttacgct tacgcccgcc
     1201 gctgctttgg cccgtttctc ggttatcaaa ccaacgtcct ctactggctg gcctgctgga
     1261 tcggcaatat cgccatggtg gtcattggcg taggatattt aagttacttc ttcccgattc
     1321 tgaaagaccc attggtatta accatcacct gcgtcgtggt gctgtggatc ttcgtcctgc
     1381 tgaacattgt cggtccgaaa atgatcaccc gcgtacaggc cgttgctacc gtgctggcgc
     1441 tgatccccat cgtcgggatt gccgtatttg gctggttctg gttccgtggt gaaacctata
     1501 tggcggcatg gaacgtcagc ggcctgggca ccttcggtgc gattcaaagt acccttaacg
     1561 ttacgctgtg gtcgttcatc ggtgtagaaa gtgcctccgt tgccgcaggt gtggtgaaaa
     1621 acccgaaacg caacgtccct atcgccacca ttggcggggt attgattgct gccgtttgct
     1681 atgtactttc taccaccgcg attatgggga tgatccctaa tgccgcactg cgcgtttctg
     1741 cttcgccatt cggtgatgcc gcacggatgg cgttgggtga caccgccggg gccattgttt
     1801 ccttctgcgc agctgcgggt tgcttaggtt cactgggcgg ctggacgttg ctggcgggtc
     1861 aaacggcgaa agccgctgcc gatgacggac tgttcccacc gatttttgcc cgtgtaaata
     1921 aagcgggcac gccagtggcg gggctgatta tcgtcggtat tttgatgacc atcttccagc
     1981 tcagtagcat ttcgccaaac gcgaccaaag aattcggtct ggtttcttcc gtgtcggtca
     2041 tctttacact ggtgccatat ctttacacct gtgcggcgtt actgctgctc ggccacggtc
     2101 actttggtaa agcacgcccg gcatacctgg cagttactac cattgccttc ctctactgca
     2161 tctgggccgt agtagggtcc ggcgcgaaag aggttatgtg gtcatttgtc actctgatgg
     2221 taatcaccgc catgtatgcg ctgaattaca accggctgca taaaaacccg tatcccttag
     2281 atgcaccaat aagcaaagat taattcccct taatcaagca aacataaaag ccaaccttaa
     2341 gaacttaagg ttggcttaat tttgctttgc gagcatatgc gcactttgtt cgatggaaac
     2401 accgtgatgt tgaagcgcct actaaaaaga ccctctttaa atttattctc ctggttatta
     2461 cttgctactt tttatatctc agtctgcctg aatatcgcct tttttaaaca ggtgctacag
     2521 gtgttaccgc tggattcact gcataacgtg ctggttttct tgtcgatgcc ggtcgtcgca
     2581 ttcagcgtga ttaatattgt cctgacacta ggctctttct tatggcttaa tcgtccactc
     2641 gcctgcctgt ttattctggt cggtgcggct gcacaatatt tcatcatgac ctatggcatc
     2701 gtcatcgacc gttcaatgat caccaatatc attgatacta cgccggcgga aagttatgcg
     2761 ttgatgacgc cacgaatgtt attaacgctg gggttaagtg gcgtgttggc cgcgattatt
     2821 gcctgctgga tcaaaatcaa acccaccacc tcacgcctgc gcagcgttct tttccgcggg
     2881 gccaacatcc tgatttctgt actgctgatt ctgctggttg ccgcactgtt ctacaaagac
     2941 tatgcctcgc tgtttcgcaa caacaaagaa ctggtgaaat ccttaagccc gtccaacagc
     3001 attgttgcta gctggtcgtg gtactctcac cagcggtcag ccaacctgcc actgatacgc
     3061 attggtgaag atgctcaccg caacccatta atgcaaaatg ggaaacgcaa aaacctgacc
     3121 attttgatcg tcggagaaac gtcgcgcgca gagaatttct cgctgaatgg ttatccacgc
     3181 gaaaccaatc ctcgcctggc aaaagataac gtggtctatt tccctaatac cgcatcttgt
     3241 ggcacagcaa cagccgtctc agtgccgtgc atgttctcgg atatgccgcg tgagcactac
     3301 aaagaagagc tggcacagca ccaggaaggc gtgctggata tcattcagcg agcgggcatc
     3361 aacgtgctgt ggaatgacaa cgatggcggc tgtaaaggcg tttgcgatcg cgtacctcac
     3421 cagaacgtca ccgcgctgaa cctgcctggt cagtgcatca acggcgaatg ctatgatgaa
     3481 gtgctgttcc acgggctgga agattacatc aataacctgc agggtgatgg cgtgattgtc
     3541 ttacacacca tcggcagcca cggcccgacc tattacaacc gctatccgcc gcagttcagg
     3601 aaatttaccc caacctgcga cactaacgag atccagacct gtaccaaaga gcaactggtg
     3661 aacacttacg acaacacgct ggtttacgtc gactatattg ttgataaagc gattaatctg
     3721 ctgaaagaac atcaggataa atttaccacc agcctggttt atctttctga ccacggtgaa
     3781 tcgttaggtg aaaatggcat ctatctgcac ggtctgcctt atgccatcgc cccggatagc
     3841 caaaaacagg tgccgatgct gctgtggcta tcggaggatt atcaaaaacg gtatcaggtt
     3901 gaccagaact gcctgcaaaa acaggcgcaa acgcaacact attcacaaga caatttattc
     3961 tcaaccttat tgggcctgac tggcgttgag acgaagtatt accaggctgc ggatgatatt
     4021 ctgcaaactt gcaggagagt gagtgaatga aaattctgat tgttgaagac gatacgctgt
     4081 tattgcaggg attgattctg gcggcgcaaa ccgaaggcta cgcgtgcgat ggcgtgagca
     4141 ccgcgcggat ggcggaacaa agccttgaag ccggtcatta cagcctggtg gtactggatt
     4201 taggcttacc cgatgaagat gggctgcatt ttcttgcccg tatccggcag aaaaaataca
     4261 ctctgccggt gttaattctc accgcccgcg atactcttac cgacaaaatc gccgggctgg
     4321 atgtcggggc cgacgattat ctggtgaagc cttttgcgct ggaagagttg cacgcccgta
     4381 tccgcgccct gctacgacgc cataataatc agggcgaaag tgagctgaac gttggcaacc
     4441 tgacgctgaa tatgggtcgc cgccaggtat ggatgagcgg agaagaactg attctgacgc
     4501 ccaaagaata tgctctgctg tcgcgcctga tgcttaaagc aggcagcccg gtgcatcggg
     4561 aaattctcta caacgatatc tacaactggg acaacgagcc ctcgaccaac accctggaag
     4621 tgcatatcca taacctgcgc gacaaagtgg gcaaagcccg tatccgcacc gtgcgcggat
     4681 ttggctatat gctggtcgca aatgaggaaa actaattgaa tctgatgcgt tttctgcgcc
     4741 gaccaatatc gctgcgccaa cggctgatat tgaccatcgg ggccattttg ttggtgtttg
     4801 agctgatcag cgtcttctgg ctatggcatg aaagtaccga gcagattcag ctgtttgagc
     4861 aggcgctacg cgacaatcgc aacaacgatc gccacatcat gcgtgagatc cgcgaggcgg
     4921 tcgccagcct gattgtcccc ggcgtcttta tggttagcct gacgctattt atctgctatc
     4981 aggcggtacg ccgcatcacc cgaccgctgg cggagctgca aaaagagctg gaagcgcgca
     5041 ccgccgacaa cttaacgccc attgccattc atagcgccac cctcgaaatc gatgcggtgg
     5101 tttcggcatt aaacgatctg gtcagtcgcc tgaccagcac gctggataac gaaaggttgt
     5161 ttaccgctga cgtcgcgcac gaactgcgaa cgccactggc gggggtgcgt ttgcatctgg
     5221 aactgctggc gaaaacgcat cacattgatg tagcaccgtt agtggcacga cttgatcaga
     5281 tgatggagag cgtctcccaa ctgctgcaac tggcgcgtgc cggacagtca ttttcttccg
     5341 gtaattatca acatgtaaaa ctgctggaag atgtcattct cccctcgtat gacgaactca
     5401 gtaccatgct cgaccagcgc cagcaaaccc tgctattgcc agagagcgcc gccgacatca
     5461 ccgttcaggg tgatgcaacc ctgctgcgga tgttattgcg aaacctggta gaaaacgccc
     5521 atcgttatag cccacaaggc agcaacatta tgattaagct gcaagaagat ggcggagcgg
     5581 tcatggccgt tgaagatgaa ggaccgggta ttgatgagag taaatgcggg gagttgagta
     5641 aagcgtttgt acgaatggac agccgttatg gcgggattgg tctggggtta agtattgtca
     5701 gccgcattac acagttgcat cacgggcagt ttttcctgca aaaccggcaa gagacttccg
     5761 gcacgcgggc ctggatacgg ctgaaaaaag atcagtacgt ggcaaaccag atataaagaa
     5821 agctgctgac caccagcacg ctgaacacgg tagttaaact ttcataaaca cggtttttca
     5881 ttgcactctc ctccgttaac ctggaggaga gtatgcgcgt cagagattaa gtgaacctta
     5941 agagttcaat tgacctgata agacagcgtc acatcaggcc atccgtttca gcttattcat
     6001 caattcgcgg atgttgctgc accaggcggg tacgtttcgc ctgcaaatcg gcaatctcgt
     6061 ggtcaatatc atcgattttc tgctcgatat tatcgtaatg ctcgacgaga atttccttcg
     6121 cttcctgtat atctgacgcc gctggtgtcg cacctttcaa cggtcgattc gccgtctctt
     6181 tcatggttac gccggtgatt aaaccaacca ccgccactac catcaggtaa taggcaggca
     6241 tcatcagatt ctgcgagctt tcgaccagcc aggccgccag cgttggcgtc agaccggcaa
     6301 ccagcaccga aatattaaat gccgccgcca gcgcgctgta acggatatgc gtcgggaaca
     6361 tcgcaggcaa ggtagaagcc ataacgcccg taaagcagtt aaggatcacc gccagcatca
     6421 ataagccggc aaaaatcagg ccgatgacgt tactgttaat cagaataaac gccgggatcg
     6481 ccaacacaaa cagtgcaaca ctaccaagaa gcacaaacgg acgacggcca aaacggtcac
     6541 tcagcaagcc catcaccggt tgaacaaaca gcataccgat cataatggcg ataataatca
     6601 gcaccccgtg gtcttcggag taatgcaggt tatgcgacaa ataactcggc atataggtca
     6661 gcaacatgta gtaagtcacg ttggtggcaa ttaccagacc aatacatgtc aacaggctgc
     6721 gccagtgttt ggtggcaatc tctttaaacg agactttcgg gccatcctgc aaaccttcgc
     6781 ggtcgccctg ttccagttta tcgacatgct gctggaacgc cggagtctct tccagcgcat
     6841 ggcgcaggta aagcccgata atccctaacg gcagagcgat aaagaacgga atacgccagc
     6901 cccaatcgag gaagttctct tcgccgacaa tggtcgaaat taacaccact acgccagcac
     6961 ccagcacaaa cccggcaata gaaccaaagt ccagccagct gcccataaag ccacgtttac
     7021 ggtcagggga gtattccgca acaaatattg acgccccggt atattcaccg ccgaccgaga
     7081 aaccttgtgc catcttacag atcaacagca gaatcggtgc ccaaatacca atcgtgtcgt
     7141 aggacggtat taagccaata cagaacgtac tgatcgacat aatcacaata gtgatagcga
     7201 ggatcttctg gcgaccatat ttatcgccca acataccaaa gaagagcccg ccaagcggtc
     7261 gaatcagaaa gggaacggag aaagtggcga gtgcagcaac catctgcacg ctggggtcag
     7321 cccccgggaa aaaaacttta cctaatgcgt aagcaacaaa accataaaca ccaaaatcga
     7381 accattccat tgcattaccc agtgatgctg cggtaatggc tttacgcagt ttaccgtcat
     7441 caataatggt gacatcacga agggtaatcg gttttacttt tttccttttc agcatagctt
     7501 tcctcgcaga gttggcccat ttccgcaaac gccagaaaaa tcagcgcgac ggccgcaaaa
     7561 ggggagcggg aattgcagga tgcaatctct gtaacaagcg tagcgggttt gcttacaccc
     7621 tccggttaag gaatgtacaa tcaaaccagt tgacgcctga attgggcagt taatgacctt
     7681 tttaccctac cagtgtttaa atttgtgatc aacttcacac atgcatttcg tttaggactc
     7741 attggtgtaa ctgattattt acaccaaatt tacagagaag atttcctcga ccattgcccc
     7801 cgccagacgc tgcgctgaac agagtcgtgg catccccagt gcgacagttc gccagcgttg
     7861 ggtcactgac cagcaaacgg gttgtttatc tgccgtagac cactttccca gccaacttaa
     7921 aaaatcagca tgctccccgc ccgtcgaaac ggtcgaagag gcaaaccact gatggtaata
     7981 gtgacgcgtg gtcggggccg cattgatact ctctgccaaa tgttgtgtcg ctttcacgcg
     8041 caaatcaccc agcaacaacg ccaccgcttg cgggcttccc agacggcagg catcgccaaa
     8101 tgccccccag cgcaactcct ccagaacgat aaagcagcgc ccaggcaacg accaggcgtt
     8161 atacgcccca gcacgccagc gggtaaaaat ttgctcgctc tgttcacccg cctgcaccaa
     8221 taggcctcgt tgcgttaacg ccttttcctt ccagcgacaa cgggctgaaa accactcata
     8281 caaggcgaca atttgctggg ggagctccgg gaaagcagag tgctggcggt gatccatttt
     8341 ttgcagcagc gccagatgct gcgcgatctg cgtcagcgcc agatgaccgg gatccagcat
     8401 cgacgccagt ttctccacca atgtcagacg cagactaagc ggcgcattta acatgccgcc
     8461 gttcgtccat gtctgaagct gcacgcgcgc aagaagttcc tgcatcagtc gttcacgaaa
     8521 ctgctgctga tgggcttgtg gaaggcgggc atcatcgccc tgcgccagat ccactaaaaa
     8581 gcgcggatga accgactcca gcacgcgacc ggggccgtcc agtaacgtct tggtcaatat
     8641 cgttctgccg tgaaaagtgt ttgaatatca tcgcataaca gctgagcgtc ggtgtaaatc
     8701 cagccgtgag tcatcacagt ctgctgcaat tgctgttgca tcagcctgac caccgattca
     8761 ttttgttgac gcagagccat actttcgcgt aaacgcgtct gtaattccgt caaacatgaa
     8821 gcgaactcag cgaaaaaagt attcatgcct gccgtaacag attcatcgac ctgctctgcc
     8881 ataactttag ccatttgttg gcaataaaga tcgacttctg cgcttaatgc tcgttgcaac
     8941 acactgtaat caaccgtctc tgtcgggaat ttctcatttc cccgtcccca gtcgggctga
     9001 ttcaaccagc gcgaaaaaat ctcacgcaca acgcctaaac gcgtgctctg ctcgtccgtt
     9061 gctgcctggc gcgaaatgac ttcactgaac agctggcgag tgttgaagtg gggaactacg
     9121 ccgtgaaaaa caggaaaatg aaacccagga cgaaacccag actcactcaa ttccattttg
     9181 acttgttgct caatggggcg aataacatcg gttaacaccc ggcaaagggt ggactcaagc
     9241 tcggcaaagc gcagcgtaaa gtcgcgactg atggtgttct gcgccgtctg taacagtgtc
     9301 tcacagcggg tacgcatctc acttaacgac tccgaattat cctgaaacaa cgcggctaac
     9361 tgcgcattca ccgcatcttg ctgttgacgc agaaagtggt tggcggaggt cagggccaac
     9421 tcgatttcat gtttaatctc gccgctcacc tgcgcctgat tgagttgcaa tagctgcaaa
     9481 ctttcttcga cctgatggat attttgccgc aattgttcac aagcgacgtt taacccgtgc
     9541 gcacgaaaat ccaggtattc ccgcgcctgc tgcgcgtaat tcaacagttt atgcgcggca
     9601 gatcgcagag catacaacga ggcgttagcg taagcggcat gaagcaacgc ctgaattggc
     9661 tgggcgaaca gcgaatcttc ccacaactga tcggcagcat ggcgaatatg ttcgaggtcc
     9721 gccagatcgg catgacgcca gcgcctgccg agcgcggcat gggcaaaatc ttccacccag
     9781 tgttgttgct ctggcgcggg taacttaccg ttgttggcta actcatggcg cgcccgattc
     9841 gccaggtagc cccacatcga cgacaccgga aatatctgct gtggcgtaat acagcctttc
     9901 atcagcgttc cggaaatcag tgcccgcacc tggtcagcat cgtcactgtt acgatcctgt
     9961 tgatcgaact tattgaccag cacatacaac ggcaccgatt gccccaccgc caaaattgcc
    10021 tcacggacct cttcatcgga gatcgatttc agttgcgtat aatccagcac cgccagtacc
    10081 gccgaggcgc gtgccagctg ctggttaagc attttttgca gatgcggttg cccggcttca
    10141 tttggcccgg gggtatccag taacgtcaac tgaccgggat aactctccag ccccgccaga
    10201 tggacaaact ccacttcaat cacgggaata tgctcaatgg cggcgtaagc agaaaaagga
    10261 aaatcgacgt ccagcgcctt cgccagtcgc accaaatcat tcaaactttt cagacaatga
    10321 aaaataggct gagcacccag ataatatttt tcgaaagcga cgccattttc gatccgctgc
    10381 ataagcgcac gcatatcttt atctatttcc agcacatcgg tcagatgctt aatatcgcaa
    10441 tcacgcaggc gctgttgtaa ttgttgaatt aaatggtcga ttggcgcgac atgtgaaaaa
    10501 tgcagtaccg gttctttttg cccgggcgta tggcgaataa gcgtcggcag cgcagtcatt
    10561 gggcgattgc gattaggcag aacctccgta ccaacaatgg cattaatggt ggttgatttc
    10621 cctgctttca tagtaccgac aatcgccagc accatttcca gtcgggaaat tttacgcaac
    10681 tcattattca gcatcgcgtg acgttcggcg atattaggct gactccaggg taaagccagt
    10741 tgtggcgcat cgtctccggg tacagagaga ggcatttttt ccagtaactg caactgttgg
    10801 cgagaaagct gtaacaggcg ttcagcctcc tgacttaact catacagggt ctgtgtgtac
    10861 atagaaaatt tttccttaaa gcaaatttta ttacttttat ttagccagat tgtttttgag
    10921 ttctgttttc ggct
//
LOCUS       DADSZD010000143        10825 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.144, whole genome shotgun sequence.
ACCESSION   DADSZD010000143 DADSZD010000000
VERSION     DADSZD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.144"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            172..885
                     /locus_tag="J1Z28_003461"
     CDS             172..885
                     /locus_tag="J1Z28_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBA7718264.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            917..1675
                     /locus_tag="J1Z28_003462"
     CDS             917..1675
                     /locus_tag="J1Z28_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718265.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISRF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1721..3073
                     /locus_tag="J1Z28_003463"
     CDS             1721..3073
                     /locus_tag="J1Z28_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBA7718266.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTIRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLIAQTIAS
                     LAYVILLFMFCHLFGVMGAAFAYFGGQCLDVILSLAPTLRTFFRRHLLVYNGVGEKFQ
                     "
     gene            3070..3894
                     /locus_tag="J1Z28_003464"
     CDS             3070..3894
                     /locus_tag="J1Z28_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718267.1"
                     /translation="MMRKYFPLEVIERLFIAVDDNDVIDTQVSLPPTITLRCSPEIIH
                     DNYALCLRFWLDGVNRKELLRLTLKQAKGKILTDDERKTYKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVCLGHFQDGFRNGKKNIVSYYGNLLRVYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRHSYDAPMALDSNIRQRLELLISRFPL"
     gene            3906..4466
                     /locus_tag="J1Z28_003465"
     CDS             3906..4466
                     /locus_tag="J1Z28_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBA7718268.1"
                     /translation="MSSRLIIALVIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     EDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWHLQEQSSTILVLNKTGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            4497..5567
                     /gene="lptF"
                     /locus_tag="J1Z28_003466"
     CDS             4497..5567
                     /gene="lptF"
                     /locus_tag="J1Z28_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBA7718269.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFPR"
     gene            5564..6643
                     /gene="lptG"
                     /locus_tag="J1Z28_003467"
     CDS             5564..6643
                     /gene="lptG"
                     /locus_tag="J1Z28_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBA7718270.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGYSIFRIALV
                     ALVAGILWTVSLGAIDEWGASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDYLAWQSTFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWHDRQH"
     gene            complement(6678..7850)
                     /locus_tag="J1Z28_003468"
     CDS             complement(6678..7850)
                     /locus_tag="J1Z28_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBA7718271.1"
                     /translation="MGLYDKYARLAGERLQFSDYGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISTLADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(7850..8098)
                     /locus_tag="J1Z28_003469"
     CDS             complement(7850..8098)
                     /locus_tag="J1Z28_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBA7718272.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(8130..9044)
                     /locus_tag="J1Z28_003470"
     CDS             complement(8130..9044)
                     /locus_tag="J1Z28_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBA7718273.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETLLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPLPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(9041..10774)
                     /locus_tag="J1Z28_003471"
     CDS             complement(9041..10774)
                     /locus_tag="J1Z28_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBA7718274.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAW
                     FKALPEADVVLQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRP
                     GDRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSV
                     APPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTF
                     MPCYGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKAL
                     PEHGIEIRNEADIPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYL
                     LDGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQC
                     RISDEARRGQLIHALAARIQSEFGVTADIDLLPPHSIPRTSSGKPARAEAKKRYQKAY
                     AASLHVQESLA"
BASE COUNT         2647 a         2654 c         2807 g         2717 t
ORIGIN      
        1 tatatcctta aatttaaata tccatccaaa atatttattt ggttaatatg tttttgatga
       61 aagcgtaatt caggtcaatg tcacaattaa ccattgtcac aataaggttg aacggatatt
      121 ctgagcacgg accacgtaat attcaataca ttatttgctg ccgggttttt cgtgaatcaa
      181 cgtaatatgt caataattaa ttccacgcca gtgcgtgtta ttgccattgt aggatgtgat
      241 ggttcaggta aatcgaccct cacggcaagc ctggtaaatg aactggcatc aagaatgcca
      301 acagaacaca tttatctcgg gcaatcgtcc gggcgaattg gcgaatggat ttcacagcta
      361 cctgttattg gcgcaccttt tgggcgttat ttgcgaagta aagcggcaca tgtgcacgaa
      421 aagccctcaa caccgcctgg caatattact gcgctggtta tctatctgct ttcctgctgg
      481 cgggcgtaca agtttcgcaa aatgttgtgt aaaaaccagc aaggctatct gctcatcacc
      541 gaccgctacc cgcaggttga agtgccgggt ttccgctttg atggcccgca attggcaaaa
      601 accacgggcg gtaacggttg gataaaaatg ttaaggcagc gtgagctgaa gctgtaccaa
      661 tggatggcgt cttatttgcc tgtattgttg attcgtcttg gcattgatga acaaaccgcg
      721 tttgcgcgta aacctgacca ccaactggca gcgttacagg aaaaaatcgc cgttacgccg
      781 caactgacat tcaatggcgc caggatcctt gagctggatg gtcggcaacc agccgatgaa
      841 attctgcaag cgtcactacg cgcaattcac gccgcgcttt cctgatcacc aaaatggata
      901 ggggatgatt aaacgaatgg atgcactaca aacacaaact gttaagagta caaccgcgcc
      961 gcagcccaac tacattccgg ggctgattgc ggtggtcggg tgtgatggaa ccggtaaatc
     1021 cacactgaca aatgacctgg tgaaatcgct gcaacaacac tggcaaaccg agcggcgcta
     1081 tctggggctg ctctccggcg aagacggcga caaaatcaaa cgattgccgt tggttggtgt
     1141 ctggctcgaa cggcgactgg cggccaaatc ctcgaaaacc caaagcatga aaaccaaatc
     1201 tccggcgcta tgggcggcgg tgattatgta ctgcttctcg ctgcgaagaa tggcgaatct
     1261 gcgcaaggtt cagcgactgg cgcaaagtgg cgttctggtg gtcagcgatc gcttcccgca
     1321 ggctgaaatt tcgggctttt attatgacgg accggggatt ggcgtcgaac gtgcgaccgg
     1381 gaaaatcagc aggtttctgg cgcagcgtga acggcgttta taccaaaaaa tggcgcaata
     1441 ccgtcctgaa ttaatcattc gcttggggat tgatattgat actgccatct cccgcaagcc
     1501 tgaccatgac tatgccgagc tgcaggacaa aatcggcgtc atgtcgacga ttggctataa
     1561 cggcacaaaa attcttgaga ttgattccag agcgccttac agcgaggtgc tggaacaggc
     1621 acagaaggcg gtttctctgg ttgccatcgt ttctgaccgc cgaagtttaa cttaaggcag
     1681 gcgggatact ttttactatc aacgataagg tcaggctgaa ttggcgggtt ttaacatcaa
     1741 acattggttt gcagatggcg cgtttcgcac cattattcgc aatagcgcct ggttaggctc
     1801 cagtaatgtt gtgagcgctt tgctggggct gttggcgctc tcgtgtgccg gtaaagggat
     1861 gacgcccgcc atgtttggcg tactggtaat tgtgcaatcg tacgccaagt cgatcagcga
     1921 ttttattaag tttcagacat ggcagctggt ggttcagtac ggaacgccgg ctttgaccaa
     1981 caataatccg cagcaatttc gcaatgttgt ctcattttcc ttctcgctgg atatcgtcag
     2041 cggcgcggtg gcgattgtcg gcggcattgc cttactgcca ttcctttccc attcattagg
     2101 tctggatgac caaagctttt ggctggcggc gctctattgc acgctcattc cttcaatggc
     2161 ttcctccacg ccgaccggca ttctgcgtgc ggtagatcgc tttgatttga ttgctgtaca
     2221 gcaggcgacg aaaccttttc tgcgcgcagc ggggagcgtc gtagcctggt attttgactt
     2281 tggttttgcg ggttttgtta ttgcctggta cgtgtcgaat ctggttggcg gcaccatgta
     2341 ctggtggttt gccgcgcgcg aattacgccg cagaaatatc cataacgcct tcaaattgaa
     2401 tctgtttgag tctgcccgac acattaaagg cgcgtggagt tttgtctggt caaccaacat
     2461 tgcccactcc atctggtcgg cgcgtaactc gtgcagcaca gtgttggtgg ggatcgtgtt
     2521 aggacccgct gctgccgggt tatttaaaat cgccatgaca ttcttcgatg ccgccggaac
     2581 gccagcgggt ttactgggca aaagttttta tccagaggtg atgcgtttag atccacgcac
     2641 catcagaccg tggttgttag gcgtgaagtc cggtttactg gcgggcggaa tcggtattct
     2701 ggtggcgctc gcggtgttga tcgtcggcaa gccgctcatt tcgctggtgt ttggcgttaa
     2761 gtatctcgaa gcgtatgacc tgattcaggt gatgctaggc gcaattgtga tttcgatgct
     2821 gggcttccca caggagtcat tgctattgat ggcgggaaaa cagcgtgctt ttttgatagc
     2881 acaaactatt gcatcgttag catacgttat tttgttgttt atgttttgtc atctgtttgg
     2941 cgtaatgggg gctgcatttg cttatttcgg tggtcaatgt ctggatgtga tcctgtcgtt
     3001 agcaccgaca ttaaggactt ttttccgtcg ccatttattg gtctataacg gagtcggaga
     3061 aaaattccaa tgatgagaaa atactttcca cttgaggtca ttgagcgttt atttattgct
     3121 gttgatgata atgatgttat tgacacacag gttagcttgc cgccaacgat cactttgcgc
     3181 tgttcaccgg aaattattca tgacaattat gccttatgtt tgagattctg gctggatggt
     3241 gtaaatcgta aagagttact tcgcttaacg ctgaaacaag caaaaggcaa aatacttaca
     3301 gacgatgagc gcaaaacgta taaatacatg cgagcgcgct ataagcatct gcgtttcgct
     3361 cagcgactgt atctgaaaaa acaccaggca ggatttttat tcggcaaaac gacggtatgt
     3421 ctgggccatt ttcaggatgg tttccgtaac ggtaagaaaa atattgtgtc gtattacggc
     3481 aacctgttac gggtttattt aagctctccg gtctggtcgt tggtgaacta ttcattacgc
     3541 catagccagt tagaaagcgt aagcggtttt attgcctatc ggcaaaagca aatgcatacc
     3601 ctgaaagaga taattgccaa accgcgacta accggcaggg aatttcatga tgtgcgaaaa
     3661 atcatcagcc agcaggtttc ttattacgac accttaagat cgcttgatcc tgaaaataaa
     3721 gaagcgttac agatttcccg cttcctcgcc gccattaatg gcctgatggg ggataaacat
     3781 gacgacatgg tggcggatga tatggaaaac cgccattcgt atgatgcacc gatggcgctg
     3841 gatagcaata ttcgtcagcg cttagaactg ctgatttcgc gttttccgct ataaccggag
     3901 caatgatgtc atctcgtttg attattgcgc tggttatcat gctcttagcc ccgggtgtgc
     3961 aggcgcacaa tttcgtcact ggtaaaacgg ttacccctgt ttatatccag gaaggcggcg
     4021 agctgcttct gaatagcgaa gacgaaattc actaccaaaa gtggaacagc acacagctgg
     4081 cgggaaaagt ccgtattatt cagtatatcg ccgggcgtaa gtcggcgaag aaaaagaact
     4141 cactgttaat taaagcggtt gaagcggcaa actttccgca ggatcgtttt cagcccacca
     4201 ccattgtgaa cactgatgat gccatctttg gtaccggcta ttttgtcgtt ggcaaaatcg
     4261 aaaaaaataa acgccgctat ccatgggcgc aattcgttat tgacggcaat ggacagggac
     4321 gcgttgcctg gcacttgcag gagcagagtt caaccattct ggtgctgaat aaaaccgggc
     4381 aaattcagtg ggcaaaggac ggcagtctga cgccggaaga ggtagatcat gtcattgcgc
     4441 tggcgcagaa actgatcaat gaataaaaaa ttcaaaggat tgagggcgtt agtgagatga
     4501 agctggttga acactacatc atgcgtggta cgcgccgtct ggtgttgatt atcgtcggtt
     4561 ttctgatctt tattttcgcc agctactccg cacagcgtta tctgaccgaa gcggcaaatg
     4621 ggacattagc tcttgatgtt gtgctggata tcgttttcta caaagtgctg attgcactgg
     4681 agatgttgtt acctgttgga ctgtatgtgt cagttggcgt gacgctaggg caaatgtata
     4741 ccgactcgga aatcacagct atctctgcgg cggggggcag tccgggacgc ttgtacaaag
     4801 ccgttcttta tctggcgata ccgctaagta tttttgtcac ccttctgtcg atgtatggtc
     4861 gtccgtgggc ttatgcgcag atttatcaac tggagcaaca gtcacagtcg gagctggatg
     4921 ttcgccagtt gcgggcaaag aaatttaaca ctaacgataa cggacgaatg atcctttccc
     4981 agacggttga tcaggataat aatcgcctga ctgacgcgct gatttatact tctactgcca
     5041 atcgaacccg cattttccgc gcccgttcgg ttgatgtggt tgacccatct cccgagaaac
     5101 cgaccgttat gttgcacaac gggaccgcct atcttctcga tcatcagggg cgtgacgaca
     5161 acgaacagat ctaccgtaat ctgcaattac atctgaatcc gctggatcaa agccctaacg
     5221 tcaaacgcaa agcaaaatcg gtcacggagc tggcgcgctc cgtctttcca gccgatcatg
     5281 ccgaactgca atggcgacaa agccgtggcc tgaccgcatt gttgatggcg ctgctggcca
     5341 tttcattaag tcgggtaaaa ccgcggcaag ggcgattttc aacgttattg ccactgacat
     5401 tgctgtttgt tgccattttt tatggcggcg acgtctgccg tacgctggtg gctaacggcg
     5461 cgattcccct cattcctggt ttgtggttag tgcccggact catgctaatg ggcctgttga
     5521 tgctggtcgc acgcgacttc tccttgctgc agaaatttcc ccgatgaatg ttttcagtcg
     5581 ctatttaatc cgtcacctct ttctcggttt tgccgccgcc gcagggctat tgctgccgct
     5641 ttttaccacc ttcaacctga ttaacgaact ggatgatgtc agtccgggcg gttatcgctg
     5701 gacccaggcg gtgctggtgg tgctgatgac cttaccgcgc acactggtcg aactttcgcc
     5761 gtttatcgcc ttattgggag gtattgtcgg tctcgggcag ctatcgaaaa acagtgagct
     5821 taccgccatt cgcagcacgg ggtattctat cttccgtatt gcactggtgg cgctggttgc
     5881 cgggatactg tggactgttt cgttaggcgc gatagatgag tggggggcgt cgccattgca
     5941 gcaacaggcg ctgcaaatca aatcgaccgc caccgcgttg ggggaggatg atgacattac
     6001 cggcaatatg ctttgggcca ggcgcggtaa tgaatttgtg acggtgaaat cgctgaacga
     6061 gcagggccag ccggtgggcg tggagatttt tcattaccgc gacgatctct cgctcgaatc
     6121 ctacatttat gcgcgcagtg ccaccattga agatgacaaa acgtggatcc tgcatggtgt
     6181 gaatcataaa aaatggctga atggcaaaga aacgctggaa acatctgatt atcttgcctg
     6241 gcaatcgacc ttcaccagta tgaatcttga agagttatcg atgccgggga atactttttc
     6301 tgtccgtcag cttaatcatt acatccatta tttgcaggaa accgggcaac ccagcagcga
     6361 ataccgcctt gcactgtggg aaaaactggg gcaacccatc ttaaccctgg cgatgatttt
     6421 gctggctgtg ccgtttacct ttagtgcccc gcgctcgccg gggatgggta gccgtctcgc
     6481 tgtaggggtc atcgttggtt tactcacctg gatcagctat caaatcatgg tcaatctggg
     6541 attgctattt gcgttgagcg cacctgttac cgcgctcggt ttaccggtag cgtttgtgct
     6601 ggtggcgttg agcctggtgt attggcatga cagacaacat taaacccgcc gtggcgcaac
     6661 accacggcgg gttcacatta ctgcttcaga gtagcaaatg cctgaatgat ggcatcgatt
     6721 tgctcacgac tgtgggcagc gtttacgcta cagcgcagca gcgtgatgcc agcaggcgcg
     6781 gcaggtggca ggataagatt cacataaacg cccagcgaaa taagctcgcg ccagatgcgt
     6841 aaaccttcct ctttcgaacc gattattact ggcactaccg ggctaatatg tgaacttaac
     6901 tcgtaaccca gtttttgcag accgtcatac agatggttgg cgttatccat cagcttttgc
     6961 cgcagctctg gttgtgaggc aatggttctt aacgacgagc gcacggtcgc aatgcaggac
     7021 ggagaaggag aggcggtaaa aatatacggg cggctgccgt agcgcagcac ttccatcgct
     7081 tccgaaccga cagcaaaacc gccaatcgag gccagacttt tactgaaggt gccgacgata
     7141 atatccacgt cgtcttcaac gccaacggct tcaaccagtc cgcgccccgt tgcgcctaac
     7201 acgccaaacg aatgtgcttc atcgacaatt aaatatccgc cgagacgacg tttgatatca
     7261 acaatttctg ccagcggcgc aacgtcaccg agcatactgt agatgccttc aacgataata
     7321 atcgcctctt tcgcccgttc accgaggcgg accatgcgcc gttccagatc gttgacgtca
     7381 ttatggcgaa aacgaataat ctccgcgccg ccgagagaac aggcgtcata aatactggcg
     7441 tggctgtcgg catccagtaa caccacggcg ttatggtcgg caagtgtgct gatgacgcct
     7501 aaattagcgg tataaccggt tgaaaaaacg atagcggtag ggcgattgaa aaactcggca
     7561 atctctttct ctaacgccag atgcggcgca taactgccgt ttgccatacg agaaccggtg
     7621 gtgccagtgc cttgggccgc taacgctgcc tgaccttcgc ggatagcatc atgattaaag
     7681 gttaaaccga gataattatt ggtcccggcc agaatcactt ttttattgcc gatccggcct
     7741 tcggtagcgg aatacacttc atcaatacag gtgccaaatg gtgttaaccc gtagtcacta
     7801 aattgcaggc gctcgccagc aaggcgcgca tatttatcgt atagccccat tatttattct
     7861 ccagccatgg aagtagagtt tccattaatt gcgcaggggt tttgacatcc agcagaatat
     7921 taataggaat ggaaatatcg aatctgtctt ccagcatcat taagagatcc atcactttga
     7981 tcgactccag gccaagatcg ttgacgagat cgctgtcggg cttaatttcc acaccgtttt
     8041 cgaccaaatc ctgtaaacag gaaaggatgt aatccattac tatttcacga tttaccatag
     8101 aaacgttaac acacctttaa acaagacaat caaaatagcc cgttgcgtaa tgcgtgttcc
     8161 aggctaatcc cgggaaacca attaatatct tcagaaatac ggttattact tgccgaccag
     8221 tcggcatgag ttaattcacg gattttcgaa cgagtcagca tcggttcttt acccgccaga
     8281 cggctcaacg cggtactgat atccgcaagg caggtgagta gcggcagagg aataccgacc
     8341 atccgcacgg aaccacagcg gacatcggca acaagctgtt ggacacgttg ccagtcatag
     8401 cctccggcga cgccatcgca taattcatag gtttgcgtct gtacagtttc ggcgcttaac
     8461 cactgaccca ctgcttgcgc aaaatcggtg acgtgaagaa aagagagcag tgtctctggc
     8521 gtgccaagtc gtggcagcag accgcgcagc atccagtcaa acagcggttt taactcttta
     8581 tcgccgggac catacacggc tgtcgggcga aaaacgccca acgtaatttc gtcagccatt
     8641 gcagtcagcc gttgttcggc gacgtgtttg gaatttgcgt accaggagag ttcgggatgg
     8701 cgcgccgcca gcgaagagat aaacagaaaa cgttggcaaa agccgctctc ttttgccgct
     8761 tgcatcaggc gcaggctgcc gtcaacgttg cagcgggtaa aaatctcttc tttgtgcccg
     8821 cgcacttgcc cggcgcaatg gaccaccgcg ctggctccgg caaccagttc gctaagcgaa
     8881 tgtgtatctt ccagcgaacc gcgcacccaa gtgagattat cattgacgtg agcgcgggca
     8941 gtacgcgtca atgcgcgaac gtgaaagccg cgggcgagca ggttatcaat aatatattta
     9001 ccgataaacc cggtagcgcc cgtcaccgcg acagtttgat tcatgccagg gattcctgca
     9061 catgaagact ggcagcataa gccttctgat aacgtttttt cgcttccgca cgggcaggct
     9121 tgccggagga tgttcgggga atactgtggg gcggcaacag atcgatatcc gcggtaacgc
     9181 caaattcgct ttggatccga gctgccagcg cgtggataag ctgcccgcga cgtgcttcgt
     9241 cgctgatccg acactggatc tgcaaaatga ttttttcctg ggcggtaaca aaagcaatcg
     9301 catcgccaga atgaatttcc ggctcctgtt ccgcaatgta ttcaatatcc tgcggccaga
     9361 tattacggcc acgaataata atcagatctt taatgcgtcc ggtgacatac agataaccgt
     9421 ccagcagata accaaggtcg ccggtgtcta accagcccgt cgcggcaatc tcgtcttgcg
     9481 aaacctggtc gccaaagtac ccgctcatca ggctggggcc ggagatgcaa atatggccta
     9541 ccacacgttc cgcgacaggt atatctgctt cattgcggat ttcaatgcca tgttccggca
     9601 acgctttacc gcagttgacg aaagtcgata cggcgcgtgt ttcagcaccc ggcgcgacgg
     9661 ctttaccctg gtattcgagg atgtcgcgat ccacttcgtt aaccacaacc ccgcaggctt
     9721 catcagagaa gctgacagcc agcgcatttt ctgccagtcc gtagcacggc atgaaagttt
     9781 tatcgtcaaa gttaacctga cggaaacatt cagcgaattg atggagttgt tctgcggata
     9841 tgggttctgc gccaatacca gcgacgcgcc agcaggaaag atccagttca gcgagatctt
     9901 tttcattcac gcggcgctgg cacaattcat agccaaacgg cggtgcaacg gaaacggtgc
     9961 cacgattttt actgatcaat ttaagccatt gcagaggacg catggcaaaa tcctgagtgc
    10021 gcaaataatc tactgaaagc tgcgtggcga cgggggtcag gagaaagccg accagtccca
    10081 tatcatggta gaaaggcagc caggagacgc agcggtcgcc agggcgtaat ttaatcccat
    10141 cgtggcttat agcacgtaga ttagccatca cttcgtgatg ggtgataatg acgccacggg
    10201 gaaaacgggt gctgccggag gtgtactgga ggtaggcgat atcgtttgga actggacgct
    10261 ggagcacaac atcggcttcc ggtaatgcct taaaccaggc gtggcttaaa acatgtaatt
    10321 cggggttgtt gttatgcgtc gcggcattga ccagtggcaa ccactcatca ccagtgataa
    10381 tggctgcggg ctggcaactt gccagtaaac cctgcagttt ggcgctccag gaatcccgct
    10441 gacctacgcc cattggaatc gccaacggga cggcgactaa gccggcatac tggcaggcaa
    10501 aaaaagcctc tacaaacccg ctactcgttt cggcaatcag cgccacgcga tcgccttttt
    10561 tcaggttcag cgataacaac cttttcgcac cagcttcggc acgcgctttt aacgtctgat
    10621 attccagttg atcttcaagt tggcaacgtc tgtcataaaa attcattccg gcgctactca
    10681 gagcggcgta gtccaaagca tcaaccagcg ttggaaaatc ggcatagcgc ataggtaggg
    10741 aatgcgtaaa gattttatta gacatataca ccacgaaaaa attggatatc aaaaataaaa
    10801 tgacaataat gcggtggatt atata
//
LOCUS       DADSZD010000144        10664 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.145, whole genome shotgun sequence.
ACCESSION   DADSZD010000144 DADSZD010000000
VERSION     DADSZD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.145"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(141..1193)
                     /gene="fbaB"
                     /locus_tag="J1Z28_003472"
     CDS             complement(141..1193)
                     /gene="fbaB"
                     /locus_tag="J1Z28_003472"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBA7718275.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1450..2727
                     /locus_tag="J1Z28_003473"
     CDS             1450..2727
                     /locus_tag="J1Z28_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718276.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TTAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2724..3728
                     /locus_tag="J1Z28_003474"
     CDS             2724..3728
                     /locus_tag="J1Z28_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBA7718277.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDIDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAI
                     "
     gene            3725..4690
                     /locus_tag="J1Z28_003475"
     CDS             3725..4690
                     /locus_tag="J1Z28_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7718278.1"
                     /translation="MSGARLHTLLPELATRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4664..5410)
                     /locus_tag="J1Z28_003476"
     CDS             complement(4664..5410)
                     /locus_tag="J1Z28_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7718279.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRNGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5462..6280)
                     /locus_tag="J1Z28_003477"
     CDS             complement(5462..6280)
                     /locus_tag="J1Z28_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBA7718280.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(6345..7145)
                     /gene="thiD"
                     /locus_tag="J1Z28_003478"
     CDS             complement(6345..7145)
                     /gene="thiD"
                     /locus_tag="J1Z28_003478"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBA7718281.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKNWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7142..7930)
                     /gene="thiM"
                     /locus_tag="J1Z28_003479"
     CDS             complement(7142..7930)
                     /gene="thiM"
                     /locus_tag="J1Z28_003479"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBA7718282.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLAQEVQA"
     regulatory      complement(7965..8061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8153..8425)
                     /gene="rcnR"
                     /locus_tag="J1Z28_003480"
     CDS             complement(8153..8425)
                     /gene="rcnR"
                     /locus_tag="J1Z28_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBA7718283.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8546..9370
                     /locus_tag="J1Z28_003481"
     CDS             8546..9370
                     /locus_tag="J1Z28_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBA7718284.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9589..9927
                     /gene="rcnB"
                     /locus_tag="J1Z28_003482"
     CDS             9589..9927
                     /gene="rcnB"
                     /locus_tag="J1Z28_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBA7718285.1"
                     /translation="MTIKNKMLLGALLLVTSATWAAPVTAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10008..>10664)
                     /locus_tag="J1Z28_003483"
     CDS             complement(10008..>10664)
                     /locus_tag="J1Z28_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBA7718286.1"
                     /translation="GGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDACVNSY
                     RNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGNKTTINSKKGD
                     IILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGTPVTLL
                     NITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD
                     "
BASE COUNT         2486 a         2723 c         2692 g         2763 t
ORIGIN      
        1 aatatgaaaa ttaatcagaa tgacaggcaa aaaaaacggc ctcccggggg aagccgtaac
       61 gaagtgcgcg tgtttttatg ccggatgcaa cgtaaacgcc ttttccgtcc tacggggtgg
      121 tgctgttcgt atgaggcgta tcaggcgata gtaattttgc tatcgagata aacgtcctgc
      181 acggcgttaa tcagtttcac gccgtcagcc atcgatttct tgaacgcctt acgtccaaga
      241 atcagcccca ttccgcctgc gcgtttgttg ataaccgcag tacgcactgc atcgctgagg
      301 tcagtttcac cgcctgcagc accgccggag tttatcaacc cggcccggcc catatagcag
      361 ttagctaact gataacgcac caggtcaatc gggttttcac tggtcaattt gctgtagact
      421 cgatcgtcgg tataaccgta attaatcgct ttatagccgc cgttattttc cgccattttt
      481 tgtttgacga tatctgcacc tatggttgcc gccagatggt tcgcctgacc agtcaggtcg
      541 gcggaaacat ggtaatcaac gccatctttc ttaaaggcgg agttacgcaa ataggcccac
      601 agcactgtca ccatgcccag ctcgtgcgca cgttcaaaag ccgcagaaat ttcttcaatc
      661 tggcgacgtg actcttccga gccaaaatag atagtcgcac caaccgccac cgcgcccata
      721 ttgaacgcct gctccacgct ggcatacagc gtttgatcgt aggtattcgg gtaacttagc
      781 gtctcgttgt gattaagttt gacgaggaat ggaatgcgat gcgcgtaacg ccgcgatacc
      841 gatgccagca cgccgtaagt tgacgccaca cagttacagc ccgcttcgat cgccagttca
      901 acaatgtttt tcgggtcaaa gtagagcggg ttagcggcaa atgaagctcc ggcagagtgc
      961 tcaacgccct ggtcaaccgg cagaatagaa agatatcctg tgccagccag acgcccggtg
     1021 ttgtacaacg tctgcatatt acgtaacacc gctggcgggc gattattgtc aatcatcacg
     1081 cggtctacgt agtcatgtcc ggggagataa agctggtcag aaggaatagt catacaacgg
     1141 tgctgtaaaa ggttgtcggc gtctttgcca agcaactgcg caatatctgt cattactatg
     1201 ctcccgtaaa ttccgattgg atatcggcta tggattgtcc tggcccgcct tttgcgggca
     1261 atcataatcc tggtcgttac gggcgagttt ttccatcttt tggatgtttt ttcagcgttt
     1321 tctgttggct cgattcatca gaaaaaatgt taccgcggtc aagttttcac cgcaaaggta
     1381 tttaaaaggt attactaagt ggtattgtca tcgcgtacct tacattacct gtcatgaagg
     1441 aattaaaaga tgaaaacaac agcaaagctg tcgttcatga tgtttgttga atggtttatc
     1501 tggggcgcgt ggtttgtgcc attgtggttg tggttaagta aaagcggttt tagtgccgga
     1561 gaaattggct ggtcgtatgc ctgtaccgcc attgcggcga tcctgtcgcc gattctggtt
     1621 ggctccatca ctgaccgctt tttctcggcg cagaaagtgc tggcggtatt gatgttcgct
     1681 ggtgcggtgc tgatgtattt cgccgcgcaa cagaccactt ttgccgggtt cttcccgtta
     1741 ctgctggcct actcgctaac ctatatgccg accattgcgc tgactaacag tatcgctttt
     1801 gccaacgtgc cggatgtgga gcgtgatttc ccgcgcattc gtgtgatggg cactatcggc
     1861 tggattgcct ctggtctggc atgtggtttc ttgccgcaaa tgctggggta tgccgatatc
     1921 tcaccgacga acatcccgct gctgattact gccggaagtt ctgctctgct tggtgtgttt
     1981 gcatttttcc tgcccgacac accgccaaaa agcaccggca aaatggatat taaagtcatg
     2041 ctcggcctgg atgcgctgat cctgctgcgc gataagaact tcctcgtctt tttcttctgt
     2101 tcattcctgt ttgcgatgcc actggcgttc tattacatct ttgccaacgg ttatctgacc
     2161 gaagttggca tgaaaaacgc cactggctgg atgacgctcg gccagttctc tgaaatcttc
     2221 tttatgctgg cattgccgtt tttcaccaaa cgctttggta tcaaaaaggt attattgctt
     2281 ggtctggtca ccgccgcgat ccgttatggc ttctttattt acggcagtgc ggatgaatat
     2341 ttcacttacg ctctcttgtt cctcggcatt ttgctgcacg gcgtaagtta cgatttttac
     2401 tacgttaccg cttacatcta tgtcgataaa aaagctcccg tgcatatgcg taccaccgcg
     2461 caggggctga tcacgctctg ctgccagggc ttcggcagtt tgctcggcta tcgtcttggc
     2521 ggtgtgatga tggaaaagat gttcgcttat caggaaccgg taaacggact gactttcaac
     2581 tgggccggga tgtggacttt cggcgcggtg atgattgcca ttatcgccgt gctgttcatg
     2641 atttttttcc gcgaatccga caacgaaatt acggctatca aggtcgatga tcgcgatatt
     2701 gcgttgacac aaggggaagt taaatgaaaa cagaacgtat tctcggtgct ctttatgggc
     2761 aggcgttagg ggatgcgatg gggatgccct ccgagctttg gccgcgcagt cgcgtcaaag
     2821 cacactttgg ctggattgac cgttttctgc ctgggccaaa ggagaataac gcagcctgtt
     2881 attttaaccg cgccgaattc accgacgata cctcgatggc gctgtgtctg gcggatgcgt
     2941 tactggaacg tgaaggcaag atcgatccgg atctgattgg gcgtaatatt ctcgactggg
     3001 cgctgcgttt cgacgccttt aacaaaaacg tactaggtcc gacctcgaag attgcgctta
     3061 acgccattcg cgacggtaaa cccgttgctg aactggaaaa taacggcgtg accaacggcg
     3121 cagcgatgcg catctcgcca ttaggttgtt tgcttccggc gcgcgatatt gactctttta
     3181 ttgatgatgt ggcgctggcc tccagcccga cacataaatc cgatctggcg gttgcggggg
     3241 cggtagtcat cgcatgggcg atttctcgtg ccattgacgg agaaagctgg tcagcgattg
     3301 ttgattcact gccttcaatt gcgcgacatg ctcaacaaaa acgcatcact accttcagcg
     3361 cctcactggc agcacgtctg gagattgcgc tgaaaattgt gcgcaatgct gacggcaccg
     3421 aatccgccag cgaacagctt taccaggtcg ttggcgcagg taccagcact attgagtccg
     3481 ttccgtgcgc cattgcgctg gttgaactgg cacaaaccga cccgaaccgt tgcgccgtcc
     3541 tgtgcgctaa ccttggcggc gacacagaca ccatcggtgc tatggcgacg gcaatttgtg
     3601 gcgcgttgca tggcgttaac gctatcgatc ctgcgttaaa ggcggaactg gatgcggtaa
     3661 atcagcttga tttcaaccgc tatgccacgg cgctggcgaa gtatcgccaa caacgggagg
     3721 cgatatgagc ggcgctcgat tacacacgct gctgcctgaa ttagccacgc gtcagtcggt
     3781 gatggtcgtc ggcgcggcgg tcattgatgt gatcgccgac gcttatgccc tcccctggcg
     3841 tgggtgcgat atcgaactga aacagcagag cgttaacgtt ggcggctgcg cactgaatat
     3901 tgccgtggcg ttaaagcgcc tcggcatcga agcgggtaat gccttgccgc tcggtcaggg
     3961 ggtgtgggcg gagattattc gcaaccggat ggcaaaagag gacttaatca gcctgatcga
     4021 taacgccgaa ggtgataacg gctggtgtct ggcgctggtt gagcctgatg gcgaacgcac
     4081 ttttatgtca ttcagcggtg tggaaaatca gtggaatcgc cagtggctgg cgcgattaac
     4141 cgttgcgcct ggcagcctgc tctatttttc cggttatcaa ctggcctcgc cctgcggcga
     4201 attgttagtg gaatggctgg aaaagctgca agacgtgacg ccgtttatcg attttggccc
     4261 acgtattggc gatatcccgg atgcattact ggcgcggatc atggcctgtc gacctttagt
     4321 gtcactcaat cgtcaagagg ctgagattgc cgccgaacgt tttgctttat ccgcagagat
     4381 aacaacactt ggcaagcaat ggcaggagaa atttgccgcg ccgttgatcg ttcgcctcga
     4441 taaagaaggc gcatggtatt tcagcaacga cgcttctggc tgcattccgg catttccaac
     4501 gcaagttgta gacaccattg gggcgggcga cagtcatgcc ggtggcgtac ttgccgggct
     4561 ggcctctggt ctgccactgg cggatgccgt attactgggc aatgcagtgg cgtcgtgggt
     4621 tgtcgggcat cggggcggtg attgtgcgcc aacgcgcgag gaactactcc tcgcacacaa
     4681 aaacgtatag atcgctgcga cagtggctga tgctgtactc aataggccgt tgctgttggt
     4741 caagcgccac ttgcttgatc accagcaccg gtattttgct atctaactga atatgtgact
     4801 gaaactcggc atccggcatc cgggcgctaa cgcgggaacg cgtacgttgt gggtaaatat
     4861 gctggctgcg gaagtagtca taaagagaaa tcccgatggc atcaacatca tgaattaaat
     4921 gtgccggaac ccacgattcc tcaatcgata ccgcctcttc atcgacatag cgaatacgct
     4981 taagcaaaaa gacatcactt cccgcttcga ccgccagctg ctgcgcgact tcttcgggac
     5041 atttcacgac acgcttgtta acccatagcg tatcgggctt tttcccgcgc aataccacct
     5101 gctgagaaaa cccccgcgct tctttcagcg agtattcgaa gatgttgttg atctgcgtgc
     5161 cgtaaccacg cgaacgcgtc accacgccct cttcttccag cgcctgcatc gccttgcgca
     5221 ccgtaatgcg cgaaacgccg gttaactgac ttaaatcacg ctcaccgggc aaaatattgc
     5281 catgctccag caccccgttg cgcacggcat tttttaccgt ttcggcaaac ttaatataaa
     5341 gcggcgtgtt atccgccgct aaaatacgct cgttcagttg ggcaatcagc tgggtatgcg
     5401 cttgttccat ttgtcttttt cctgccgggg aatctggtgg gagtatatca ctgcttcata
     5461 tttaaggcgt ttctttaatc ccatcaacga atgcctgcaa ctcctccacc gtgccattaa
     5521 acacattaaa atccaccgga ccattaatgc catcgacctg tccacggtcg gaatgctgcc
     5581 agaagcgcca ggccatgccg tcattgtccg ggcgacgttg ataatagtga gccacccacc
     5641 acggatattc attgaaatag cccgccagat tggtgtgata aaaaacggct cctgagtaaa
     5701 taattggctt ttttcccgta cgtttttcga ccatttttag ccactgactt acccgcttgc
     5761 gtaattcttt tgccgataat tttccccgtt cttctacgtc cagcacggca gggaaatcgc
     5821 cttgtgagaa atccaccgtt tgcagaaata atctcgcctg aactgaagca gataccgacg
     5881 gggaaaaata atgatacgcc ccgcgcagca ggccattttc gcggcttagt tgccagttac
     5941 gcgaaaaata ggggtccacc agcttttcgc cttccgtcgc cttaataaag gcaaactgca
     6001 agcgaatacc gttatcgcgc atttttgcca cccgctgcca gtcgatccgc tcctgccagc
     6061 gcgaaacatc tattccgtga atggtgtaac tggcaggaat gcgaatagcg aaagatttaa
     6121 ccgggcgata accgtagaag tgaatgtagt ctttaatctg aattgctgtc gaatagaaaa
     6181 aattcaccgt ctgacgcgga taaattgcaa cgatggaaat tagcccaaga gcgcataata
     6241 aaacggtgaa tttttttcga ctggtgattc tcaactgcat tggtttcatc cctgaatgtc
     6301 agcaccaaag gctgacaata accaaagcag ctatagtacg gtgctcacca ccaggcgtgg
     6361 aagtggtgaa ccggaccaat accgtgacca acttccagcg tgtcggcctg ggctaatgcc
     6421 gatgaaagcc agttttttgc ctcctgtacg gtgtcagccc agtttgtatg gcgcgggcgc
     6481 agtgcagcca acgccgcaga gagcgtacaa ccagtgccgt gagtgttttt ggtcataatg
     6541 cgctgtgcgg taaaccgttg ttcaccctcg cgggtaaaca gccagtccgg gctttgctca
     6601 tcatccagat gaccaccttt cattagcact gcgccacagc ccatcgccaa cagcgatcgc
     6661 ccttgttcca gcatttcctg ttcggtgcgc gcgtgtggcg cgtcgagcaa ggcggctgct
     6721 tcgggcaagt ttggtgttat taatgaaacc tgtggcaata atcgactgcg tagcgtggca
     6781 actgccgaag gtgaaagcag cgggtcgccg ctttttgcca gcataacggt gtcgagtacc
     6841 acgttttgga tctgataacg ttgcaaccgt tctgccaccg cttcaacaat atcggtttcc
     6901 gccaacatac cgattttagt ggtgtcgatt cgcacatcgc tgaacaccga atcgagctgg
     6961 gcggcgacaa aatcaggctc aatgcgatac accgactgta cgccacgggt attttgcgcc
     7021 accagtgcag taataactga gcaaccataa gcgccaagtg ccgagaaggt tttaagatcg
     7081 gcctgaatcc ccgcaccacc actcggatca gtaccggcaa tcgtcagagc gttaattcgt
     7141 ttcatgcctg cacctcctgc gccaattgcc agagcgcatc aaggaaatgt ggaacaaaac
     7201 tgcctggccc ctcgcttctg gcaactgcgc gttctccggc ttgtttcatc cagtgacagg
     7261 cagatgcgat attttccagc gtatcgcctg gtagcgcaca acaggcagcg acaaccgccg
     7321 atagtgcaca gccagttcct accactttgg tcattaacgg atcgccaccg tgaatgccaa
     7381 tgatacgatg tccatcggta acataatcca cctcgccagt gaccacgacg attgcgccag
     7441 tttcccgtgc cagtgtttgt gcagcgggta tcgcgttagc tgcggcatca gtggtatcca
     7501 ctccccgtcc accattagca acgccagcta atgccatgat ttccgaagca ttaccacgta
     7561 tggcggttgg cttatgggac agaagttcca cacaaaaacg gcggcgataa tcgagcgcac
     7621 caaccgcaac cggatcaagc gtccagggcg tttgtgatcg tgttgcttgc tcaacggcag
     7681 cactcatcgc ttgcgctcgt ggctgcgtca gcgtgccaac gttaatcaac aaggcactgg
     7741 cgatagccgc aaactgactg gcctcttcgg tttcgataac catcgctggc gatgcgccga
     7801 gcgccagcaa ggtattggca gtaaaagttt gcaccacatc gttggtcatg cagtgcacaa
     7861 gaggggaatg ttggtgaaaa aggtgtaacg cgtgcgcaga ttgcgctgaa ctcagcaggt
     7921 cgacttgcat agtttgctcc tgccataacg tgaagaagca atgacctggt ggtccgtgac
     7981 ttccctacgc tggcattatc cagatcaggt gatacgggta tttctcagcc ttcacaaaga
     8041 agggcacccc gagtcgtttg gttgcgataa taaatcgcag aggaggatgg taatgtccag
     8101 cgcacgcgtt gtaaacgggg aaaaacggcg gtattacaat aacgttgtga aattatttga
     8161 tataagaatc cagcaccttc agaacgacat ccagatcttc ttcacgtttt agctcatccc
     8221 cctggtgaac gatgtgttcc gtcagatggc ctttaatcac ttcccgcatc agaccgttta
     8281 ccgcgccacg gatagcagca atctgttgta aaactgcagc gcattcatgc ggttcgtcga
     8341 gcattttctt gagcgccacg acctggccct gaatcttact ggcacgcgct ttcagtttct
     8401 gtttatcacg gattgtatga gacatggcaa cacctggtta acaagaatat gaaaaatcat
     8461 agcactatta atctactggg gggtagtatc agatactggg ggggagtaga atcagattgc
     8521 cgaattaata ctaagaatta ttatcatgac cgaatttaca acacttcttc agcaaggaaa
     8581 cgcctggttc ttcatcccca gcgccatctt acttggtgcg cttcatggcc tggaaccggg
     8641 gcactcaaaa acgatgatgg cggcgtttat catcgccatc aaaggcacca ttaaacaggc
     8701 ggtgatgctc ggactggcag caactatttc gcataccgca gtggtctggt taattgcctt
     8761 tggcgggatg gtgatcagca aacgctttac tgctcaatca gcagaaccgt ggctccagtt
     8821 gatttccgca gtgatcatta ttagcaccgc gttctggatg ttctggcgta cctggcgcgg
     8881 cgaacgcaac tggctggaga atatgcacga gcatgatcat gagcatcatc accacgatca
     8941 cgaagatcac cacgaccatg gacatcatca ccatcacgaa catggcgagt atcaggatgc
     9001 ccatgcacga gcccatgcca atgacattaa acgacgcttt gatggtagag aggtcaccaa
     9061 ctggcaaatt ttgctatttg gcttaaccgg tggccttatc ccctgcccgg cagcaattac
     9121 cgtgctgttg atttgcattc agttgaaagc cctgacactg ggcgcaacac tggtcgtcag
     9181 tttcagcctc ggcctggcgt taacgcttgt caccgtaggc gttggcgcag caatcagcgt
     9241 tcagcaggtc gcaaaacgct ggagcggatt taacactctc gctaaacgcg ctccctattt
     9301 ttccagtctg ttgattggct tagttggtgt gtatatgggc gtacatggct tcatgggcat
     9361 aatgcgataa ctcaatcctt taggcttgca tgttatcttc agcccaggat gattcttaaa
     9421 tcagctattt ccgctgactc tctgctcgct aaggatgctt ttagggcatc ctttcattta
     9481 cactttttac gaaatcatgg gatcactaac aaaatatcgc ttgtcagtta tattgtatgg
     9541 caggaaagat atgcgactga tattacagat ccccaaagtg gagagtttat gaccattaaa
     9601 aataagatgt tgctgggtgc gcttttgctg gttaccagtg ccacctgggc cgcaccagtc
     9661 accgcgggtt cgaccaatac ctcgggaatt tctaagtatg agttaagcag tttcattgct
     9721 gactttaagc atttcaaacc aggtgacacc gtcccagaga tgtaccgcac cgatgagtac
     9781 aacattaagc agtggcagtt acgtaacctg cccgcgcctg atgccgggac gcactggacc
     9841 tatatgggtg gcgcgtacgt gttgatcagc gacaccgacg gtaaaatcat taaagcctac
     9901 gacggtgaga ttttttatca tcgctaaaaa aagccccctc ggagagaggg gggaatgcag
     9961 acaccttaat atttttatta ttagccactt gctcgtcttg cttgttatta gtcgtatttc
    10021 acgttgatta atgcggttgc ctccagtgcg ccagatttaa ctttgtttgt atcgtagacg
    10081 tagtaactgg ccgttatcgg aattgaaacc gttgtatctg taagttttga ctttgccgca
    10141 atttctttta aatttgtata tcctttactg ctgttagtga tatttaaaag tgtgactggc
    10201 gtaccattac tttcaagaat aaatcctacg ccattatctt ccgcaccttt caggatcgtg
    10261 ttgctgttgg ttaacaagtc agaacttgat aaatacacct gcattttccg tgatgtttga
    10321 ttttgttgac ctaataaatt tttacaacgc agaataatat cccctttttt actatttatt
    10381 gttgttttgt tacctgtggc tggtaattgt gaaacgggaa tactctctaa cgtaattaat
    10441 aaatcgtccg gtttgcaggt tgtggttatc gggttgtatg acaatgtcag attaaaggaa
    10501 tagacacctg cattttgtgc caatgcattt ctgtagctat ttacacatgc atcccatgac
    10561 ttaccaccta ataaacacag tagaaattgt actgcggcac tcccgcgtgt cgatgatgaa
    10621 gcactggata atgtcgcaac tccattaatc atcactgaac cacc
//
LOCUS       DADSZD010000145        10647 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.146, whole genome shotgun sequence.
ACCESSION   DADSZD010000145 DADSZD010000000
VERSION     DADSZD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.146"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..371
                     /locus_tag="J1Z28_003484"
     CDS             <1..371
                     /locus_tag="J1Z28_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBA7718287.1"
                     /translation="ILSIDELSRLLFVWLTFIGAIVAYMDNSHVQVTFLVEKLSPANQ
                     QRVSLLTHTLILLLCLGLAWGAIEKTAQDWSNLSPILGVPIGLMYAAAIPTSLIIALL
                     ELRHLYRQFTNTPSRNQQGA"
     gene            374..1651
                     /gene="yiaN"
                     /locus_tag="J1Z28_003485"
     CDS             374..1651
                     /gene="yiaN"
                     /locus_tag="J1Z28_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBA7718288.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGELMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1664..2650
                     /gene="yiaO"
                     /locus_tag="J1Z28_003486"
     CDS             1664..2650
                     /gene="yiaO"
                     /locus_tag="J1Z28_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HBA7718289.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2654..4150
                     /gene="lyxK"
                     /locus_tag="J1Z28_003487"
     CDS             2654..4150
                     /gene="lyxK"
                     /locus_tag="J1Z28_003487"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HBA7718290.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLQPDMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4147..4809
                     /gene="ulaD"
                     /locus_tag="J1Z28_003488"
     CDS             4147..4809
                     /gene="ulaD"
                     /locus_tag="J1Z28_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA7718291.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTRLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAGDFHAQIDSIWGGK
                     RA"
     gene            4802..5662
                     /locus_tag="J1Z28_003489"
     CDS             4802..5662
                     /locus_tag="J1Z28_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HBA7718292.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5656..6351
                     /gene="araD"
                     /locus_tag="J1Z28_003490"
     CDS             5656..6351
                     /gene="araD"
                     /locus_tag="J1Z28_003490"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA7718293.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AKIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6398..7936)
                     /locus_tag="J1Z28_003491"
     CDS             complement(6398..7936)
                     /locus_tag="J1Z28_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBA7718294.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8044..9339)
                     /locus_tag="J1Z28_003492"
     CDS             complement(8044..9339)
                     /locus_tag="J1Z28_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBA7718295.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMAEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLIRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKRINNQQAVYVAHYPL"
     gene            complement(9469..10620)
                     /gene="yiaY"
                     /locus_tag="J1Z28_003493"
     CDS             complement(9469..10620)
                     /gene="yiaY"
                     /locus_tag="J1Z28_003493"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBA7718296.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLAANALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         2453 a         2837 c         2803 g         2554 t
ORIGIN      
        1 gtatattatc gattgatgaa ttatcccgac ttttatttgt ctggttaacc tttattggcg
       61 caatcgttgc ttatatggac aacagccatg tgcaggttac ttttttagtc gaaaaactct
      121 caccggctaa tcaacaacgt gtttcgctgc taacccatac gctcatttta ttgctgtgtc
      181 tggggctggc ctggggcgct atagaaaaaa cagcgcaaga ctggagcaac ttatcgccca
      241 ttcttggcgt ccccattggc ttaatgtacg ccgccgctat ccccaccagt ctgattatcg
      301 cattgctgga attgcgtcac ttataccggc agttcacaaa tacccccagc cgaaaccaac
      361 aaggagcctg acgatgacaa tccttatatt tattgtcagt ttgttagggg ctatcgcaat
      421 tggcgttccc atcgcctggg cattattact gtgcggcatt tcattgatgt tctggatgga
      481 tatctttgat gtgcaaattc tggcacaaac gttggtaaat ggcgcagaca gcttctccct
      541 gttagccatc cccttctttg tactggcggg tgagctcatg aacgcgggcg gcctgtcgca
      601 acgtatcgtc gacttgccaa tgaaactggt aggccacagg ccaggcggtc tgggttacgt
      661 gggcgtgctg gcagcgatga tcatggcaag cctgtccggt tctgcggttg ctgataccgc
      721 agctgtcgcg gcgctgctgg tgccgatgat gcgccaggcc aactatccgg taaaccgcgc
      781 cgccgggctt atcggttccg gggggattat tgcggccatt atcccgcctt ccataccact
      841 cattatcttc ggcgtctcca gcggactctc catcagcaaa ctgtttatgg cgggcattgc
      901 gccaggcatc atgatgggcg tcacgctgat ggttacctgg tggtggcagg caaaacgact
      961 gaatttacct tgtcagccga aagcctcact gcgagaagtc tggcaatcct tagtttccgg
     1021 tatttgggcg ctctttttac cgataattat tattggcggt ttccgttcgg ggttatttac
     1081 accgaccgaa gcaggcgcgg ttgccgcctt ctacgctctg ttcgtctcgg tggtggtcta
     1141 tcgcgagatg accttctcca cgctttatca tgtgctgatt aacgcggcaa aaaccacatc
     1201 cgtggtgatg ttcctggtgg cctctgctgc ggtatctgca tggcttatta cgatcgccga
     1261 attacccatg atggtatcgg agctattaca accgttggtt gattcgcctc gcttattatt
     1321 catcgtcatt atgctcgcta ttatggtgat cagtacggtc atggacttaa cgcctaccgt
     1381 attaattctt acaccagtat taatgccatt agtgaaagaa gcgggaatag atccggtcta
     1441 tttcggcatc atgtttatta tcaactgttc aatatcactg attacgcctc cagtcggtaa
     1501 tgttcttaac gtggtatgcg gcgtggcgaa acttaaattt gacgatgcgg taaaaggcgt
     1561 tgcgccttac gtcatggttt tattcatgtt gttagcatta tttattttta taccggagct
     1621 cattaccgct cctcttaaat ggatgagtta aaaggaaatc ataatgaaat tacgttctgt
     1681 aacctacgct ttattaattg ccggagtggc gacgttcagc acatcttctc tggcggcaca
     1741 atcattacgt ttcggttatg aaacctcaca aaccgattca caacatattg cggcgaaaaa
     1801 atttaatgaa ttactgcagg aaaaaaccaa aggcgaactg aaattaaaac tgttcccgga
     1861 tagcaccctt ggtaatgcgc aggcgatgat cagcggcgtg cgtggcggca ctattgatat
     1921 ggagatgtct ggctctaaca actttacggg actctcgccc gtcatcaacc tgctcgatgt
     1981 cccgttcctg ttccgcgata ccgctcacgc gcataaaacg ctcgacggca aagtcggtga
     2041 tgacctgaaa gtctcccttg aaggtaaggg gctgaaagtg ctggcttact gggaaaacgg
     2101 ctggcgcgat gtcaccaact cgcgtgcacc ggtcaaaacg cctgccgacc tgaaagggtt
     2161 gaaaatccgc accaacaaca gcccgatgaa tatcgctgca tttaaagttt ttggcgctaa
     2221 cccgatcccg atgccgtttg ccgaagtgta taccgggctg gaaacccgca ctatcgacgc
     2281 tcaggaacac ccgatcaacg tcgtctggtc agcaaaattt tatgaagtgc agaagtacct
     2341 ttccctgacc catcacgcct attcgccgct tctggtggtg atcaacaaag cgaagtttga
     2401 tggcttaacc ccagagttcc agcaggcact gatctcatct gcgcaggaag cgggtagcta
     2461 tcagcgcaaa ctggtcgccg aagatcagca aaaaatcatc gacggtatga aagaagcggg
     2521 cgtggaagtc atcaccgatc tcgaccgcaa agcctttagc gatgcgctgg gtactcaggt
     2581 ccgtgacatg tttgttaaag atgttccgca gggcgctgat ctgctgaaag ccgtggatga
     2641 ggtgcaataa accatgacgc aatactggct ggggttagat tgtggcggta gctggctgaa
     2701 agccgggctg tatgaccgag aaggccggga ggcgggcgtg cagcgcctgc cgctgtgcgc
     2761 attaagtcca cagccaggct gggcagagcg cgacatggca gagctgtggc aatgttgcac
     2821 tgctgtcatt cgcgccctgc ttacccattc cggcgtcagc ggggaacaaa ttgtcggtat
     2881 cggcatctcc gcgcagggaa agggcttatt tctacttgat aaaaacgaca aaccgctcgg
     2941 taacgccatt ttgtcctcgg atcgccgggc gatggaaatc gttcgtcgct ggcaggaaga
     3001 tggcatcccg gaaaaactct atccgctgac ccgacaaacc ttatggaccg ggcatccggt
     3061 gtcgctgtta cgctggctga aagagcacga accagaacgc tacgcgcaaa ttggctgcgt
     3121 gatgatgacg cacgactacc tgcgctggtg tttaaccggc gtcaaaggtt gcgaagagag
     3181 caatatttct gagtccaatc tctacaacat gagtcgcggg gaatatgacc cgtgccttac
     3241 cgactggctg gggatcgctg aaatcaatca cgccctgccg cctgttgtcg gatctgccga
     3301 aatctgcggg gaaatcaccg ctcagacagc cgtactgacc ggtctgaaag cgggtacccc
     3361 cgttgtcggc ggcctgtttg atgtggtttc caccgcactc tgcgccgggc tcgaagacga
     3421 attcaccctc aatgcggtga tggggacctg ggcggtgacc agcggcataa cccacggttt
     3481 acgtgacggt gaagcgcatc cgtatgtcta tggtcgctac gttaacgatg gtcaatttat
     3541 cgttcacgaa gccagcccca cctcttccgg caatctcgaa tggtttaccg cacagtgggg
     3601 agaaatctca tttgctgaga ttaatcaggc cgttgccagt ttgccgaaag ccggtggcga
     3661 tctctttttc ctgccgttcc tgtacggcag caacgccgga ctcgaaatga ccagcggttt
     3721 ctacgggatg caggccattc acacccgtgc acacctctta caggctatct atgaaggggt
     3781 ggtgttcagc catatgaccc atctcaaccg gatgcgcgaa cgttttactg atgtgcacac
     3841 cctgcgcgtc actggcggcc cggcgcactc cgatgtctgg atgcaaatgc tggcggacgt
     3901 cagcggtctg cgtatcgagc tgccgcaggt ggaagaaacc ggctgctttg gtgcggcact
     3961 tgccgcccgc gtcggtaccg gggtttatcg cgatttcagc gaagcccaac gtgacttaca
     4021 gcacccggta cgcaccctgc agccggatat ggccgcgcat cagctttacc aacaaaaata
     4081 ccaacgctat caacatctca ttgccgcact tcagggttat cacgcccgca tcaaggagca
     4141 cacattatga gtcgaccact tctgcaactg gccctcgacc actcatcact tgaagccgcg
     4201 cagcgcgacg tgacgcggtt aaaagacagc gtcgatattg tcgaagcagg caccattctc
     4261 tgtttaaacg aagggcttgg cgccgtgaaa gccttgcgcg aacagtgccc tgacaaaatc
     4321 atcgttgccg actggaaggt cgccgacgcg ggtgaaacgc tcgcgcaaca ggcatttggc
     4381 gcaggcgcta actggatgac catcatctgc gccgcgccgc tcgccacggt ggaaaaaggc
     4441 cacgcgatgg cacagaactg cggcggtgaa attcagatcg agctattcgg caactggacg
     4501 ctggacgatg cccgtgactg gcatcgtatt ggtgtacgac aggcgattta tcatcgtggt
     4561 cgtgacgcgc aggccagcgg gcaacagtgg ggcgaggccg atctggcacg catgaaagcg
     4621 ctttccgata tcggccttga gctttccatt actggcggga ttacccctgc tgacctgccg
     4681 ctgtttaaag agatccgcgt gaaagcgttt attgccggac gggcgctggc aggcgcagcc
     4741 aatccggcgc aagtggctgg cgatttccat gcgcaaatcg actccatctg gggaggcaag
     4801 cgtgcgtaat catccgttag gcatttatga aaaagcgctg gcgaaagatc tctcctggcc
     4861 ggagcggctg gtactggcaa aaagctgtgg ttttgatttt gtcgaaatgt cggtggatga
     4921 aaccgacgaa cggttgtcac gtcttgactg gagtgccgca caacgtactt cgctggttac
     4981 cgcgatgatc gaaacgggtg ttgctattcc gtcaatgtgc ctgtccgcac atcgtcgttt
     5041 tccttttggt agccgggacg acgccgtgcg ccagcgggcg cgggagatta tgagtaaagc
     5101 gattcgcctg gcgcgcgatc tcggcattcg tactattcaa ctggcaggtt acgacgtcta
     5161 ttacgaagac catgacgaag gcacccagca acggtttgct gaagggctgg cgtgggcggt
     5221 tgaacaggca gcggcatcac aagtcatgct ggcggtggag attatggaca ccgcgtttat
     5281 gaattctatc agcaaatgga aaaagtggga cgaaatgctt gcgtcaccgt ggttcactgt
     5341 ttacccggac gtaggcaacc tcagcgcctg gggcaatgat gttcccgccg aactgaaact
     5401 gggcatcgac cgtatcgccg ccatccacct gaaagacact caacccgtta ccgaacatag
     5461 ccccggacag ttccgcgatg tgccgtttgg cgaaggctgt gtcgattttg ttggcatctt
     5521 caaaacgctg catgagctga actatcgcgg atcatttctg attgaaatgt ggaccgaaaa
     5581 agccaaagaa ccggtgctgg agattattca ggcgcggcgc tggattgaag cgcgtatgca
     5641 ggaggccgga tttatatgtt agagcaactg aaagctgacg tgctggcggc aaacctggcg
     5701 cttcccgttc atcatctggt gacgttcacc tggggcaatg tcagcgcgat agacgaaacg
     5761 cggcaatgga tggtgattaa gccttccggc gttgagtatg aggtaatgac cgccgacgat
     5821 atggtggtgg ttgagatagc cagcggcaag gtggtggaag gcagcaaaaa gccctcttcc
     5881 gacacgccaa cgcatctggc gctttaccgt cgctttgcgg agattggcgg catagtgcat
     5941 acccactcgc gccatgccac catctggtcg caggccggac ttgatctccc cgcctggggc
     6001 actactcacg ccgattattt ttacggagcc atcccctgca cgcgcctgat gaccgcagaa
     6061 gagatcaacg gcgagtatga ataccagacc ggcgaagtga tcattaaaac cttcgaagag
     6121 cgtgggttga acccggcaaa aatcccggcg gtactggtgc attctcacgg cccgttcgcc
     6181 tggggaaaaa atgccgccga tgcggtgcat aacgccgtgg tactggaaga gtgcgcctat
     6241 atgggtctgt tctcgcgcca gcttgcgcca cagctccctg cgatgcaaaa cgaactgctg
     6301 gataagcact acctgcgtaa gcatggggcc aatgcctatt acgggcagta gccatacctg
     6361 atagtgcccg gcaatacacg ccgggcagcc tttcacatca gaacagcccc aacggtttat
     6421 ccgagtagct caccagcagg cacttggttt gctggtaatg ctcaagcatc attttgtggg
     6481 tttcgcggcc aatgcctgat tgtttgtagc cgccaaacgc cgcatgtgcc gggtaagcgt
     6541 gataacagtt ggtccacacg cgccctgcct gaatgccgcg ccccatctta taggccagat
     6601 taccgttgcg gctccagacg cccgcgccca gaccatattg cgtatcgttc gccagctcca
     6661 gcgcttcttc catcgttttg aaggtggtta ccgccagcac cgggccaaaa atctcctcct
     6721 ggaacacgcg catattgttc tgaccaaaca gaatcgtcgg ttcgaggtag tagccgtctt
     6781 tcagttcgcc ttccagcagc ttgcgtcgcc cgccggtgag cacatcagcg ccctcttttt
     6841 taccgatatc aatgtagttg aggatggttt ccagttgccc gtgagaaacc tgcgcgccca
     6901 tttgcgtcac gctgtcgagc gggttaccgc tgcgaatgct ttcgacacgg cggatggcgc
     6961 gttccataaa gcgttcgtag atagactcct gcactaaagc acggcttgga caggtgcaaa
     7021 cttcgccctg gttaaaggca aacagtgcaa agccttccag cgctttatcg aaaaaggcat
     7081 cttcttcatc catcacatca gcaaagaaga tatttggcga tttgccgccc agctccaggg
     7141 ttaccggaat aatattttgc gtggcgtact gcataatttg ttggcccact tccgttgagc
     7201 cggtaaacgc cactttggcg atgcgtttcg aggtcgccag atattcgcca atttccccac
     7261 cagcgccgtt aaccacgttc accacgcccg gcggcagtaa atcaccgacg atttccatta
     7321 gcagcagtac agaaagcgga gtaagacgtg cgggtttcag caccacacag ttgcccgccg
     7381 ccagcgcggg tgccattttc cagctcgcca tcagcagcgg gaagttccac gggataatct
     7441 gccccaccac gcctaacggt tcatggaaat gataggccac ggtttcgcta tccacttcac
     7501 tgatcccacc ttcctgcgcc cgaatacacg aggcaaaata gcgaaaatga tcaatcgcca
     7561 gcggcacgtc ggcagcactg gtttcgcgaa tgggtttacc gttatcccag gtttctgccg
     7621 tcgctaaaag ttcaaggttt tgttccattc gatcggcaat cttaaacaga atcgccgcgc
     7681 gatcctgcac cgaggtgtgc gcccatttat ctttcacttt atgcgcagca tccagcgcca
     7741 gatcgatgtc tcgtttgccc gaagacgcca cttcgcacag tagctgcccg gtcaccggcg
     7801 tcaggttctg gtaatactcg ccatcggcag gcgctaccca ttcgccaccg ataaagttgt
     7861 cgtagcgggc ttttaatttg agggggaaac catactcgcc gggcttaatc tgtgctgaag
     7921 ggggattatt ggtcatgatc gtctccttgc ggtgtgaggt ataacaaggg tagacgtgac
     7981 tggcgaaatc ttcgccagtc ggtaacagct ttacgacggc tatcacgaat ttacgggcaa
     8041 aagctacagc gggtagtgtg cgacgtacac tgcttgctgg ttattaattc ttttctcttg
     8101 cgcgaatcct tctttaacaa ttgtccgtaa atcagctcga gccgtattat cagagacgcc
     8161 caagctcgcg ctaatttcag caactgtgta ctctttcccc ggattggcga gcatcacatt
     8221 caggagcgtt acctggcgcg gattcaggcg tttaagcaca ccagagctaa acagcacact
     8281 atcgagtttt gcgcttcgtt gtgtaatttc atcgatatgc tgctgccagt tttgcagagc
     8341 acgtttaatg acacccgcct gatattcaag gaaataagtc agatccatac catctgattc
     8401 agtgtactgg tagctggcgg catatttgac cggtgcagca tgaagcaaac ggctgatgga
     8461 aatatattta aagacatcgt agcccgattt cagcatatac cagtaaaaca gcgcccgact
     8521 ggtgcgcccg ttgccatccc gaaacggatg ttcatgggcc agcataaaat gcagaatgca
     8581 ggcacggatt aacgggtgaa tatacccttc atggctgttt aaccactggc aaactttttc
     8641 cagccgttcc ggtaacaatg ccgcagcggg cggttgatgc actatattgc catcatagtc
     8701 ggcgatcaca acatcatctg tttcacgaaa ttctcccgga cgatatttcg cgtcattgat
     8761 tccacccata ccaatggcat gtaactggcg aataagcgca ggtgtcagcg gttctgcgag
     8821 taagtgcgga atttccgcca tcagccgggc atttcccgca atcatatgct cgtcttcagt
     8881 gcgtggcgac cgccctgttt ccagcatttt tctcgccacc agcgttgtag tattcgcacc
     8941 ttccagctgg gagctggtga tcggctcttc caggcgtaac tggcttaact cggctccggc
     9001 cccgcgtaac cgttcagtct gatctttcag cgccagcgcg gtagcacgtt tgtcgacaag
     9061 ctcgcacgct tctgcaatca ccggggtaat gttgaatccg gcctgttcac ccgcttcatt
     9121 gcaataatta attcgttgga tcgccgcgtt gcgcgccaga cgggtaagcg tccacgccac
     9181 atcgacgttt tctccttttt tgatgcggta ctgcaactca tcaaaaggca gataacgtcc
     9241 tttttcatcg gtaacccggt taaagcgcag gtgggccagg agttcctcac ccgataagtg
     9301 tgataaagga ataagtccgg gaggtgattt gatagccatg tgcttatctc aaaaaagcgc
     9361 aatattgaga tagtgtatag caggaaagag aaactatctc aaaaaaagca agttttgaga
     9421 tagttgaaaa agcggctgac aactcgccag ccgttgtggg aatgatgatt acatcgctgc
     9481 gcgataaatc gccacaattt cttcgtgagt tgcctggatc gggttagtaa agccgcaggc
     9541 atctttcagg gcattagccg ccagaaccgc gaaatcttct tctttcacgt tcaggtcgcg
     9601 taggcctgcc gggatatcca ctttcttcgc cagttcacgg atggcgttaa tgcaggcttc
     9661 agcaccttcc gcgtcgtttt tacctgtcac gttcacgccc attgcagcgg cacaatcacg
     9721 cagacgtgca gcggcgactt tgctgttgaa tacctgaaca tgcggcagca aaacggcgtt
     9781 acatacaccg tgtggcaggt tgtagaaacc gcccagctgg tgcgccatcg catgaacata
     9841 acccagagaa gcattattga acgccatacc ggcaaggaac tgggcataag ccattgcttc
     9901 acgcgctttc gcattactgc catcttcaac ggctaacggc aggttttcgg caatcatggt
     9961 cacggctttc agtgcacaag cgtcagtgat cggcgtggcg gcaatagaaa catacgcttc
    10021 gatagcgtgc gttaaggcat ccatacccgt tgcggcggtc agtgacttcg gcataccaat
    10081 catcagagag gagtcattga cagaaagcag cggagttaca tgtttatcca caatcgccat
    10141 tttgatgtga cgcgcttcgt cagtgatgat gcagaaacgg gtcatttcag aggccgtacc
    10201 cgccgtggtg ttgatggcga tcatcggcag ctgcggtttt gcagagcggt caacgccttc
    10261 ataatcgcgg atgtcaccgc cgttggctgc aaccagcgca ataccttttg cgcagtcgtg
    10321 tggagaacca ccgcctaaag aaatcacgct atcgcaatta ttttctttaa gtaatttcaa
    10381 acctgcagcg acgttttccg tggtggggtt aggttgggtg ccatcataaa taacgctaaa
    10441 aatattgcgt tcttccagtg ctttttgcac atcacccgcc atacccaatt tcgttaacat
    10501 gttgtcggtg acaattaagg tacgggtaaa tccataatct gccatcatat tcattgcatc
    10561 attcaatgaa tcagcaccaa taacattcac ggaaggaata aaaaaggttg aagctgccat
    10621 aacacactcc tgaaagtgta aaaaagg
//
LOCUS       DADSZD010000146        10634 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.147, whole genome shotgun sequence.
ACCESSION   DADSZD010000146 DADSZD010000000
VERSION     DADSZD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.147"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..411)
                     /locus_tag="J1Z28_003494"
     CDS             complement(<1..411)
                     /locus_tag="J1Z28_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA7718297.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELI"
     gene            805..2340
                     /gene="mdoG"
                     /locus_tag="J1Z28_003495"
     CDS             805..2340
                     /gene="mdoG"
                     /locus_tag="J1Z28_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBA7718298.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            2333..4876
                     /gene="mdoH"
                     /locus_tag="J1Z28_003496"
     CDS             2333..4876
                     /gene="mdoH"
                     /locus_tag="J1Z28_003496"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBA7718299.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            5048..5275
                     /locus_tag="J1Z28_003497"
     CDS             5048..5275
                     /locus_tag="J1Z28_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBA7718300.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5276..5650)
                     /locus_tag="J1Z28_003498"
     CDS             complement(5276..5650)
                     /locus_tag="J1Z28_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBA7718301.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(5733..6959)
                     /gene="mdtG"
                     /locus_tag="J1Z28_003499"
     CDS             complement(5733..6959)
                     /gene="mdtG"
                     /locus_tag="J1Z28_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HBA7718302.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            complement(7131..8051)
                     /locus_tag="J1Z28_003500"
     CDS             complement(7131..8051)
                     /locus_tag="J1Z28_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family Kdo(2)-lipid IV(A)
                     lauroyl/palmitoleoyl acyltransferasee"
                     /protein_id="HBA7718303.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            8276..9328
                     /locus_tag="J1Z28_003501"
     CDS             8276..9328
                     /locus_tag="J1Z28_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBA7718304.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPLFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKNKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPEPTE"
     gene            complement(9370..9945)
                     /locus_tag="J1Z28_003502"
     CDS             complement(9370..9945)
                     /locus_tag="J1Z28_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBA7718305.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IELKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(9949..10515)
                     /locus_tag="J1Z28_003503"
     CDS             complement(9949..10515)
                     /locus_tag="J1Z28_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBA7718306.1"
                     /translation="MSFTNTPERYGVISAALHWISAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
BASE COUNT         2653 a         2596 c         2769 g         2616 t
ORIGIN      
        1 tattaattcc caggccaacg tattatattt gtcatacaat gacagcccag gccaactttc
       61 cgctttccct ttgacgtatt gcagcataat aaattgcggc aatgtcagta aggggatggc
      121 tgttaacatt gggatcccta cacgttcgac acgtactttc caccattttt tcaagggata
      181 gcgtaaaaaa agcatgtagg agaagtaccc ggatataacg aaaaacacct gcatgcggaa
      241 cgagtggatg aagtcattaa aaagggtcag ccacaatgac ggttcggcgc tattcacatg
      301 ccatgtatgg ctcgaataga ttaaagaaat atgaaaaggg atccctaaca acatcagcca
      361 ggcgcggatg gagtcgagga aatattcacg ttgcgcgggt actggattca tatatggtta
      421 actaatctcg gatttttcgt cttatccctg tcgggttatg cctttaggct tgttgccata
      481 gcgacaccga cctgaccgcg ccaggcgcag gcttcaaggt ttttatgcat agcatcatcg
      541 ctaccactaa ccagaatgga agcgtctgta agacggttga taaataaatt tgctggcaaa
      601 ccctacacga agtcgatgct tctgtcttta ggagaagcac ggaaagtgaa aacggttgca
      661 atcaggtgct taatccatga gccagtgtgc tgaacgatac cgggattctg ttgtcggaat
      721 ggcaggttat ccattaaaat agatcggatc gatataagca cacaaagggg gaagtgctta
      781 ctaattatga aacataaact acaaatgatg aaaatgcgtt ggttgagtgc tgcagtaatg
      841 ttaaccctgt atacatcttc aagctgggct ttcagtattg atgatgtcgc aaagcaagct
      901 caatccttag ccgggaaagg ctatgaggcg cccaaaagca acttgccctc cgttttccgc
      961 gatatgaaat acgcggacta tcagcagatc cagtttaatc atgacaaagc gtactggaac
     1021 aatctgaaga ccccattcaa actcgagttc taccatcagg gtatgtactt cgataccccg
     1081 gtcaaaataa atgaagtgac tgccaccgca gtcaaacgaa tcaaatacag cccggattat
     1141 ttcactttcg gcgatgttca gcatgacaaa gacacggtaa aagaccttgg ttttgccggt
     1201 ttcaaagtgc tttacccgat caacagcaaa gataaaaacg atgaaatcgt cagcatgctc
     1261 ggggccagct atttccgcgt gattggtgca ggtcaggttt atggcctttc tgcacgcggc
     1321 ctggcaattg ataccgcctt gccatcgggt gaagaatttc cacgcttcaa agagttctgg
     1381 atcgagcgtc caaaaccgac tgataaacgt ttaaccattt atgcattgct tgactcgccg
     1441 cgcgcgacag gtgcttacaa attcgtagtt atgccaggac gtgacacggt tgtggatgtg
     1501 cagtcgaaaa tctatctgcg cgataaagtc ggcaaactgg gggttgcacc gttaaccagt
     1561 atgttcctgt ttgggccgaa ccaaccgtcg cctgcaaata actatcgtcc ggagttgcac
     1621 gattctaacg gtctgtctat ccatgcaggt aatggtgaat ggatctggcg tccgttgaat
     1681 aacccgaaac atttagcggt cagcagcttc tcgatggaaa acccgcaagg cttcggtcta
     1741 ttgcagcgtg gtcgtgattt ctcccgcttt gaagatctcg atgatcgtta cgatcttcgt
     1801 ccaagcgcat gggtgactcc gaaaggggag tggggcaaag gcagcgttga gctggtggaa
     1861 attccaacca acgatgaaac caacgataac atcgtcgctt actggacgcc ggatcagctg
     1921 ccggagccgg gtaaagagat gaactttaaa tacaccatca ccttcagccg tgatgaagac
     1981 aaactgcatg cgccagataa cgcatgggtg caacaaacgc gtcgttcaac gggggatgtg
     2041 aagcagtcga acctgattcg ccagcctgac ggtactatcg cctttgtggt cgattttacc
     2101 ggcgctgaga tgaaaaaact gccagaggat accccggtca cagcgcaaac cagcattggt
     2161 gataatggtg agatagttga aagcacggtg cgctataacc cggttaccaa aggttggcgt
     2221 ctggtgatgc gtgtgaaagt gaaagatgcc aagaaaacca ctgaaatgcg tgctgcgctg
     2281 gtgaatgccg atcagacgtt gagtgaaacc tggagctacc agttacctgc caatgaataa
     2341 gacaactgag tacattgacg caatgcccat cgccgcaagc gagaaagcgg cattgccgaa
     2401 gactgatatc cgcgccgttc atcaggcgct ggatgccgaa caccgcacct gggcgcggga
     2461 ggatgactcc ccgcaaggct cggtaaaggc gcgtctggaa caagcctggc cagattcact
     2521 tgctgatgga cagttaatca aagacgacga agggcgcgat cagctaaagg cgatgccaga
     2581 agcaaaacgc tcctcgatgt ttcccgaccc gtggcgtacc aacccggtag gccgtttctg
     2641 ggatcgcctg cgtggacgcg atgtgacgcc gcgctatctg gctcgtttga ccaaagaaga
     2701 gcaggagagc gagcaaaagt ggcgtactgt cggtaccatc cgccgttaca ttctgttgat
     2761 cctgacgctc gcgcaaactg ttgtcgcgac ctggtatatg aagaccattc ttccttatca
     2821 ggggtgggcg ctgattaatc ctatggatat ggttggtcag gatgtgtggg tttcctttat
     2881 gcagcttctg ccttatatgc tgcaaaccgg tatcctgatc ctctttgcgg tactgttctg
     2941 ttgggtgtcc gccggattct ggacggcgtt gatgggcttc ctgcaactgc ttattggtcg
     3001 cgataaatac agtatatctg cgtcaacagt tggcgatgaa ccattaaacc cggagcatcg
     3061 cacggcgttg atcatgccta tctgtaacga agacgtgaac cgtgtttttg ctggcttgcg
     3121 tgcaacgtgg gaatcagtaa aagccaccgg gaatgccaaa cactttgatg tctacattct
     3181 gagtgacagt tataacccgg atatctgcgt cgcagagcaa aaagcctgga tggagcttat
     3241 cgctgaagtc ggtggcgaag gtcagatttt ctatcgccgc cgccgccgtc gcgtgaagcg
     3301 taaaagcggt aatatcgatg acttctgccg tcgttggggc agccagtaca gctacatggt
     3361 ggtgctggat gctgactcgg taatgaccgg tgattgtttg tgcggcctgg tgcgcctgat
     3421 ggaagccaac ccgaacgccg ggatcattca gtcgtcaccg aaagcgtccg gcatggatac
     3481 gctgtatgcg cgctgccagc agttcgcgac ccgcgtgtat gggccactgt ttacagccgg
     3541 tttgcacttc tggcaacttg gcgagtcgca ctactggggg cataacgcga ttatccgcgt
     3601 gaaaccgttt atcgagcact gtgcactggc tccgctgccg ggcgaaggtt cttttgccgg
     3661 ttcaatcctg tcacatgact tcgtggaagc ggcgttgatg cgccgtgcag gttggggggt
     3721 ctggattgct tacgatctcc cgggttctta tgaagaattg ccgcctaact tgcttgatga
     3781 gctaaaacgt gaccgccgct ggtgccacgg taacctgatg aacttccgtc tgttcctggt
     3841 gaagggtatg cacccggttc accgtgcggt gttcctgacg ggcgtgatgt cttatctctc
     3901 cgctccgctg tggtttatgt tcctcgcgct ctctactgca ttgcaggtag tacatgcgtt
     3961 gaccgaaccg caatacttcc tgcaaccacg gcagttgttc ccggtatggc cgcagtggcg
     4021 tcctgagctg gcgattgcac tttttgcttc gaccatggtg ctgttgttcc tgccgaagct
     4081 attgagcatt ttgcttatct ggtgcaaagg aacgaaagaa tacggcggct tctggcgcgt
     4141 tacattatcg ttgctgctgg aagtgctgtt ttccgtgctg ctggctccgg tacgcatgct
     4201 gttccatacg gtcttcgtcg tcagcgcgtt ccttggctgg gaagtggtgt ggaattcacc
     4261 gcagcgtgat gatgactcca cttcctgggg ggaagcgttc aaacgccacg gctcacagct
     4321 gctgttaggg ttagtgtggg cagttggtat ggcgtggttg gatctgcgtt ttctgttctg
     4381 gctggcaccg attgtcttct cgttgatcct gtcaccgttt gtttcggtga tttccagccg
     4441 tgccaccgtt ggtctgcgaa ccaaacgctg gaaactgttc ctgatcccgg aagagtattc
     4501 accgccgcag gtgctggttg ataccgatcg gttccttgag atgaatcgtc aacgctccct
     4561 tgatgatggt tttatgcacg cggtgtttaa cccgtcattt aacgctctgg caaccgcaat
     4621 ggcgaccgcg cgtcaccgcg ccagcaaggt gctggaaatc gcccgtgacc gccacgttga
     4681 acaggcgctg aacgagacgc cagagaagct gaatcgcgat cgtcgcctgg tgctgctaag
     4741 cgatccggtg acgatggccc gtctgcattt ccgcgtctgg aattccccgg agagatattc
     4801 ctcatgggtg agttattacg aagggataaa gctcaatcca ctggcattac gtaaaccgga
     4861 tgcggcttcg caataaaaac gtagttgcct gatgcgctac gcttatcagg cctacatcgt
     4921 tcctgcaatt tattgatttt gcaagatttt gtaggtcggg taaagcgttc acgccgcatc
     4981 cggcaaaaac aacgagccaa taaaaatacc ggcgttatgc cggtattttt tacggaagag
     5041 gtatcaaatg cgtttaattg tggtgagcat aatggtgacc ttgctgagtg gctgtggcag
     5101 catcattagt cgcactatac cggggcaggg gcatggcaac caatattatc ccggtgtaca
     5161 atgggacgtg cgtgactccg cctggcgtta cgtcacgatc cttgatctgc cattctccct
     5221 ggttttcgat actttactgc tgccgatcga catacatcat ggcccgtatg agtgattaac
     5281 gttcatccca ctcatcagct gcagtgcgtc cttcctcggt atccagcggt ggctcaagct
     5341 gaaattcccc ctcgtcccat tcatgtaatg tattctcatc ttgccactcc tggcggatct
     5401 caatctcatc atagtcgccg tcaaagacac tttgcgcagc ttcaccgcta agcatgggta
     5461 aacattcacc ttcttcccct tcgtcggcaa aaaactcaac ttgccacatg atgtcgccgt
     5521 cctgcaaaac gtatttttgg gcattgaact gttgcacatt cgcgttttcg gcgtcgatgc
     5581 cggggttgtc tgcaagaaat tcttcgcgtg cagcgtcaat ggcttcttca agcgttgcgt
     5641 acatggtcat cacgattctc cctttgagtt gatgaggttt cagggaaaag gatagctgat
     5701 tctccgcttt tgcaagtatg aaaggcgaaa aatcagttcg atatctgggg gattcgacga
     5761 cgacgtagac tgttccatga atagactgcg ttgaataaca ctacgccagc ggtgacgaga
     5821 aataccgccc tgaaaccgta gttcgctgaa atcgctgctc ccatcaatgg tccggtaacg
     5881 ttgccaatat cacgaaacga ttggttatag ctgaagatac gcccggcgat ctggttgctc
     5941 gagttgtaaa ccaacagtgt ctgtacggcg gggagtaatg caccatcggc ggcaccgagc
     6001 aaaaaacgta aaatcccaag ttgtaatggc gtctgaacgt aagacattgg gatcaacagc
     6061 agtacagaaa agatcagcgc cgtaatcagg atcttttcgg ggccgattcg atcgccaagt
     6121 ttgccgagtc gtggtgcact tagcagagcc gccacgcctg gcaccgaggc gatcatgcca
     6181 ctgataaaag cgacgttact gacgttaccc gccagttcgc ggacatacag cgtcagaatg
     6241 ggggcaattg agcccgtcgc cacctggatg attaacgtgg tgacaaacag gctgagtacc
     6301 agtttcgggt ttttaagtga tgtcaccact tcccgcatat gcagcatctc ttttttgctg
     6361 accggctgga atttttctct gatgcaaaac agggtgacga aaaagcagag tatgagcaca
     6421 ctggcggtaa taaagaatac cggacgtaag ccgtagctat cggcgagcaa accgccagcc
     6481 attgggccga gcaacgcgcc gctaacgccg cctgtggaga gcgtacccag cgcccagccg
     6541 cttttattac gcggtacttg tgtggcgata agagcattag cgttggggac aaatccgcca
     6601 agtaaaccaa gaagcgcccg caggatcaaa aactgccaga tattttgtgc cagtcccatc
     6661 aacaccatca cgatgcccat gccgagagca gagcgtaata gcatgagttt tcggccttta
     6721 cggtcggcga gtccacccca aaacggtgag gcgatggccg aaaataaaaa tgtaatgctg
     6781 aagacaatac cggaccacat attcagggcg gagtggccgg taacgccaag ctgctcaacg
     6841 tagaggggta agaagggcat taccaggctg aaggcggcac cggtaagaaa acagcctagc
     6901 caggcgacga tcaggtttcg tttccagttt ataggggtgt cattttcaca gggtgacata
     6961 gcaatccgct gttggtgcgc caggcgcggt gaacataaga agaaaagata agcacactaa
     7021 ttatgcgccc gacttccagg gggcgcaatc cagagagctt ttatcgctaa atcaggggga
     7081 ttttctgtgg taatgccgga tgccattctg aagcatccag catggaagat ttaatagcgt
     7141 gaaggaacgc cttccgggcg tgttttaaag cgacggtgta accacatata ctgctctggt
     7201 gccatcatga tgcatttttc gaccactttg ttcatccacg cggcggtagt ttcggcatca
     7261 tccagcggcg gagaacactc tggcggcagt ataatcaact gatacccttt gccatctggc
     7321 ttacggcgtg gaacgaaggg caccagacat gcgccggaca tccgtgccag catccaggtt
     7381 ccggtcgtgg tcgcagcctg ctcaacggca aacaacggga cgaaaacgct tgagcgcggg
     7441 ccgtaatcat gatccggtgc gtaccagacc acttcgcctt ttttcagggc tttaatcatg
     7501 ccttttaaat ctttgcggtc gagcatcgat ttatttgagc gcaaacggcc ccaggtttgt
     7561 agccagtcaa tcagtggatt atcgttcggg cggtaaacgc caatccccgg ttcctgcata
     7621 ccaaactgcc gcgcacccag ctccagtgtc agaaaatgga tgccaactaa caggatgccg
     7681 cgtttttgcg cctgcacgtc acgaatgtgt tccatgccga tcacttccgt ccagcgggcg
     7741 attcggcggt ccggccagaa ccacgccatg ccggtttcca tcaggcccat gccaacggat
     7801 tcgaaattct tcaccaccat tttacggcgt tcttgttcgc tcatttccgg gaagcacagt
     7861 tccaggttgc gatgcacaat ttttgcgcgt cgtttcataa aacgtaacgc cagttttcct
     7921 aatccacaac cgaggcggta gataaccggg tagggcaatt gcacgaccaa ccaaagtacg
     7981 ccaataccca accaggttaa ccaataacgc ggatgaagca gtgcggtgga gaacttgggt
     8041 agattcgtca tatcaatcct gtttttcaac ctatacgggc aattgtatgt attgtcgcat
     8101 tttttcgtcc gcaaccaaaa tttgtgactg aagactgggc gaaattgccg cgcttgtaaa
     8161 taacaaataa tttttaatgc taaaatgtag cgtaaaatgt gtggatgtta attatcgata
     8221 attgctatat catgccgcgg atttttactt tcccatctcg caggaaccgt acaccatgcc
     8281 agtgttacac aaccgcattt ccaacgacgc gttaaaagcc aaaatgttgg ctgagagcga
     8341 accgcgaacc accatttcgt tttataagta tttccacatt gccgatccta aggtgacccg
     8401 tgacgcttta tatcaactgt ttaccgcgct gaatgttttt gggcgagtgt atctggcgca
     8461 tgagggcatt aacgcgcaaa tcagcgtacc tgcgagcaat gttgaaacat ttcgcgcgca
     8521 gctctatgcc ttcgacccgg ctttagaggg tttacgcctg aatatcgcgt tggatgatga
     8581 cgggaaatcc ttctgggtac tgcgtatgaa ggtacgcgat cgcattgttg ccgacggtat
     8641 tgacgatcct ctctttgatg ccagcaatgt tggtgagtat ctgcaagcgg cggaagtgaa
     8701 cgccatgctt gacgatcccg atgcattgtt tatcgacatg cgtaaccact atgagtatga
     8761 agtggggcac tttgaaaacg cgctggaaat accggcagat accttccgtg agcagctgcc
     8821 aaaagcagtt gagatgatgc aggcacataa agataaaaaa atcgtcatgt actgcaccgg
     8881 cggcattcgt tgtgaaaagg ccagtgcctg gatgaaacat aacggattca acaaagtctg
     8941 gcatatcgag ggcggaatta ttgaatacgc ccgtaaggcg cgcgagcagg gcttgccggt
     9001 gcgttttatt ggcaaaaatt ttgtttttga cgagcggatg ggcgaacgta tatctgatga
     9061 gattatcgcg cactgccacc agtgcggtgc gccgtgcgac agccatacca actgtaaaaa
     9121 tgatggctgc catctgttgt ttattcagtg tccagtatgt gcggaaaaaa acaaaggttg
     9181 ttgtagtgag atttgctgcg aagaaagcgc gttaccgcca gaggaacagc gacgccgtcg
     9241 ggcaggacgt gaaaatggca ataagatctt taataagtct cgtggacgtc tgaatacaac
     9301 actgggcatt cctgaaccaa cagaataaat atcattgccg gatgcgtgcc atccggcaac
     9361 atttcactct tacttctgct gtacgccttc cactgaaata atcagatcca cttcctgaga
     9421 agctggacct aaatctgtct tgatattgaa gtctttgagc tcgattttgc cttcggcctc
     9481 aaagcctgca cgtttaccgc cccacgggtc gtcaccctga ccaattaatt tcgcttccag
     9541 tgtgacgggc ttggttacac cattcagcgt cagatcgccg gtaatatcca gttcgtcacc
     9601 gtctttcttc acgctggtgg aggtgaatgt tgcctgtgga tattttgcgg tattgaggaa
     9661 atccgcactg cgaagatgtt tatcgcgttc ggcgtgatta gtatcgacgc tggtggtgtt
     9721 aattgtcaca ttcactttat cggcagctgg atttttttca tcaaaggtaa aagtaccgtc
     9781 gaaatctttg aaggtgccat ataaccagct atagccaaga tgctggatgc ggaagttaac
     9841 aaaggcgtgc tgaccttctt tgtcaatttt gtaatcggcg gcaaccgctg aaccggcaga
     9901 gaacatcaag gaagcgaagg ttaaaccaag caggcttttt ttcatttttt atactccata
     9961 gtcagatgac gactttccca gcatgcgctt cagagtgtcg tctttatcga tgaaatgatg
    10021 cttcagggcc ataaatccgt gcataacgga cagtactacg acgctccacg cgagccaaaa
    10081 atgcagggca ccggcaaagt cagcctgtgc gccagcgtcg gcgagggtcg cggggacgtc
    10141 aaaccagcca aaaacgctga tcggtttacc atcggcagtt gagatcagat agccgctgat
    10201 gccgatggcg aacagcaata ggtacaatgc gagatgtcca gcttttgctg caagacgagt
    10261 catgggcgaa taactcggca gcggacccgg cggtggagat atgacacgcc acagaacgcg
    10321 aataaccagc cccatcatta acaaaatacc gatgctttta tgcagttcgg gtgctttgtg
    10381 ataccagcca tcgtaataac tgagcgtgac catccacaga cctaaagcaa acatgccgta
    10441 gacaataatt gcgcttatcc aatgtaaggc cgcagatata acaccatagc gttcaggggt
    10501 atttgtgaat gacataagta catccaaaac gaatttgcga gagaaagaaa atggcgtgtc
    10561 ggagaatgaa ttgcaacaca aaaataacaa attgaatgat atttattttt atttcaataa
    10621 ttttgaatta ataa
//
LOCUS       DADSZD010000147        10560 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.148, whole genome shotgun sequence.
ACCESSION   DADSZD010000147 DADSZD010000000
VERSION     DADSZD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.148"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            107..1081
                     /gene="cysB"
                     /locus_tag="J1Z28_003504"
     CDS             107..1081
                     /gene="cysB"
                     /locus_tag="J1Z28_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBA7718307.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1376..1540
                     /locus_tag="J1Z28_003505"
     CDS             1376..1540
                     /locus_tag="J1Z28_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBA7718308.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRNPELKRKAWLAVFLGSVLFWVV
                     VALFIWKLWG"
     gene            1543..1710
                     /locus_tag="J1Z28_003506"
     CDS             1543..1710
                     /locus_tag="J1Z28_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718309.1"
                     /translation="MVGQEQLESSPFCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1824..1919
                     /locus_tag="J1Z28_003507"
     CDS             1824..1919
                     /locus_tag="J1Z28_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718310.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2069..4744
                     /gene="acnA"
                     /locus_tag="J1Z28_003508"
     CDS             2069..4744
                     /gene="acnA"
                     /locus_tag="J1Z28_003508"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBA7718311.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGKSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSVLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASRELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVDQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(4808..5398)
                     /gene="ribA"
                     /locus_tag="J1Z28_003509"
     CDS             complement(4808..5398)
                     /gene="ribA"
                     /locus_tag="J1Z28_003509"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBA7718312.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5568..6332
                     /gene="pgpB"
                     /locus_tag="J1Z28_003510"
     CDS             5568..6332
                     /gene="pgpB"
                     /locus_tag="J1Z28_003510"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBA7718313.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6481..6789
                     /gene="lapA"
                     /locus_tag="J1Z28_003511"
     CDS             6481..6789
                     /gene="lapA"
                     /locus_tag="J1Z28_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBA7718314.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            6796..7965
                     /gene="lapB"
                     /locus_tag="J1Z28_003512"
     CDS             6796..7965
                     /gene="lapB"
                     /locus_tag="J1Z28_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBA7718315.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8157..8894
                     /gene="pyrF"
                     /locus_tag="J1Z28_003513"
     CDS             8157..8894
                     /gene="pyrF"
                     /locus_tag="J1Z28_003513"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBA7718316.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVSELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMAAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8894..9220
                     /gene="yciH"
                     /locus_tag="J1Z28_003514"
     CDS             8894..9220
                     /gene="yciH"
                     /locus_tag="J1Z28_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBA7718317.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSMLEAKGMK
                     VKLAGG"
     gene            complement(9346..9564)
                     /gene="osmB"
                     /locus_tag="J1Z28_003515"
     CDS             complement(9346..9564)
                     /gene="osmB"
                     /locus_tag="J1Z28_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBA7718318.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9833..10552)
                     /locus_tag="J1Z28_003516"
     CDS             complement(9833..10552)
                     /locus_tag="J1Z28_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7718319.1"
                     /translation="MVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKLSYLRRAHGFA
                     VSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLARTLGEQKKNV
                     TIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHFSKAFIGIDGW
                     QPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVERFNRVITDSK
                     IRASDLMHLEQSKLTVHVVDI"
BASE COUNT         2632 a         2462 c         2717 g         2749 t
ORIGIN      
        1 tatagtggtt atagttagca ccttttttat tattaaatcg tattagtcac ccgcctggtg
       61 tggcgaaaaa acgatgttct gatggcgtct aagtggatgg tttaacatga aattacaaca
      121 acttcgctat attgttgagg tggtcaatca taacctgaat gtctcatcaa cggcggaagg
      181 actttacaca tcacaacccg ggatcagtaa acaagtcaga atgctggaag acgagctggg
      241 cattcaaatt ttttcccgaa gcggcaagca cctgacgcag gtaacgccag caggacaaga
      301 aataattcgt atcgctcgtg aagttctgtc gaaagtcgat gccataaaat cggtcgccgg
      361 agagcacacc tggccggata aaggctcgct gtatatcgcc accacgcata ctcaggcacg
      421 ctatgcgtta ccaaacgtta tcaaaggctt tattgagcgt tatcctcgcg tttctttgca
      481 tatgcaccag ggctcgccga cacaaattgc tgatgccgtc tctaaaggca atgccgattt
      541 cgctatcgcc acggaagcgc tgcatctgta tgaagattta gtgatgctac cgtgctacca
      601 ctggaatcgg gctatagtag tcactccgga tcacccgctg gcaggtaaaa aagccattac
      661 cattgaagaa ctggcgcaat atccgttggt gacatatacc ttcggcttta ccggacgttc
      721 agaactggat actgccttta accgcgcagg gttaacgccg cgtatcgtct tcacggcaac
      781 ggatgctgac gtcattaaaa cttacgtccg gttagggctg ggggtagggg tcattgccag
      841 catggcggtg gatccggtcg ccgatcccga ccttgtgcgt gttgatgctc acgacatctt
      901 cagccacagt acaaccaaaa ttggttttcg ccgtagtact tttttgcgta gttatatgta
      961 tgatttcatt cagcgttttg caccgcattt aacgcgtgat gtcgttgatg cggctgtcgc
     1021 attgcgctct aatgaagaaa ttgaggtcat gtttaaagat ataaaactgc cggaaaaata
     1081 atttcttgcg ttattttcgg caccttttat gtagcgaagg tgccggaata tattctcctt
     1141 tgttacttat ttgtatccgg tgcacaatat ttttatccct catatttata gggtaaatta
     1201 cctgtaaagt agtgtctaat tacacacctc gcaaactcca gttttcaaat atctatttca
     1261 ggtcaaaaga ttgaattatt cactggagac gattcgtaaa ttcgctggat ttttgactaa
     1321 agtttcttta ggatttgtct catcgtatga ttaattacac caatcgttca acgtcttgct
     1381 gataagtgat ggcgattata tgaggttagc aatgccttct ggaaatcagg aaccccgccg
     1441 caatcctgaa ttaaaacgta aagcctggct ggcggttttt cttggttctg tacttttctg
     1501 ggtggttgtc gcactgttta tttggaaatt gtggggataa ctatggtcgg tcaggagcaa
     1561 ctggagtcgt caccattttg tcagcatagt gacaatgaac cagaagcaaa acgggaatgt
     1621 tccgtcgtta ttccagacga ctggcaacta acatcgcagc aacaagcctt tatagaactg
     1681 tttgctgaag atgatcagcc gaaacaataa ttatcatcat tcttattacc catttttaat
     1741 gaattacagg gcttttaata caccgcagca ataacaggtt gagttatctc aacacaaagt
     1801 aataaccgtt aagggtgtag cctatgatca acacaaatat gaaatattgg tcctggatgg
     1861 gcgcattttc tctgtcgatg ctcttctggg ctgaactcct ctggatcatt actcactgat
     1921 ccctgacccc gctgtttgct ttgcctcaag gtttctcctc ttttatcaat ttgggttgtt
     1981 atcaaatcgt tacacgatgt ttgtgttatc tttaatattc accctgaaga gaatcagggc
     2041 ttcgcaaccc tgtcattaag gaggagctat gtcgtcaacc ctacgagaag ccagtaagga
     2101 cacgttgcag gccaaagata aaacttacca ctactacagc ttgccgcttg ctgccaaatc
     2161 actgggcgat atcacccgtc tacccaagtc actcaaagtt ttgctcgaaa acctgctgcg
     2221 ttggcaggat ggtaaatcgg ttaccgaaga ggatatccac gcgctggcgg gatggctgaa
     2281 aaatgcccat gctgaccgtg aaattgccta ccgcccggca agagtgctga tgcaggactt
     2341 taccggcgta cctgccgttg ttgatctggc ggcaatgcgc gaagcggtta aacgcctcgg
     2401 cggcgatact gcaaaggtta acccactctc accggtcgac ctggtcattg accactcggt
     2461 gaccgtcgat cgttttggtg atgatgaggc atttgaagaa aacgtacgcc tggaaatgga
     2521 gcgcaaccac gaacgttatg tgttcctgaa atggggaaag caggcgttca gtcggtttag
     2581 cgtcgtgccg ccaggcacag gcatttgcca tcaggttaac ctcgaatatc tcggcaaagc
     2641 agtgtggagc gaattgcagg acggtgaatg gattgcttat ccggatacac tcgttggtac
     2701 tgactcgcac accaccatga tcaacggcct tggcgtgctg ggctggggcg ttggtggcat
     2761 cgaagcagaa gccgcaatgt taggccagcc ggtttccatg cttatcccgg acgtagtggg
     2821 ctttaaactt accggaaaat tacgtgaagg tattaccgcc acagacctgg ttctcactgt
     2881 tacccaaatg ttgcgcaaac atggcgtggt ggggaaattc gtcgaatttt atggtgatgg
     2941 gctggattca ctaccgctgg cggatcgcgc caccattgcc aatatgtcac cagaatatgg
     3001 tgccacctgt ggcttcttcc caatcgatgc tgtcaccctc gattacatgc gtttaagcgg
     3061 gcgcagtgaa gatcaggtcg agttggtcga aaaatatgcc aaagcgcagg gcatgtggcg
     3121 taatccgggc gatgaaccaa tttttaccag tgtgttagaa ctggatatga atgacgttga
     3181 agcgagcctg gcagggccaa aacgcccgca ggatcgcgtt gcattgcccg atgtaccaaa
     3241 agcatttgct gccagtagag aactggaagt gaatgccacg cataaagatc gcctgccggt
     3301 cgattatgtt atgaacggac atcagtatca gttacctgat ggcgctgttg tcattgctgc
     3361 gataacgtcg tgcaccaaca cctctaaccc aagtgtgttg atggccgcag gcttgctggc
     3421 gaaaaaagcc gtaactctgg gcctcaagcg gcaaccgtgg gtcaaagcgt cgctggcacc
     3481 gggttcgaaa gtcgtttctg attatctggc aaaagcgaaa ctgacaccgt atctcgatga
     3541 actgggattt aaccttgtgg gatacggttg taccacctgt attggtaact ctggaccgtt
     3601 gcccgatcct atcgaaacgg caatcaaaaa aggcgattta accgtcggtg cagtgctgtc
     3661 cggcaaccgt aactttgaag gccgtatcca tccgctggtt aaaaccaact ggctggcctc
     3721 gccgccgctg gtggttgcct atgcgctggc gggaaatatg aatatcaacc tggcttctga
     3781 gcctatcggc catgatcgca aaggcgagcc ggtatatctg aaagatatct ggccatcggc
     3841 acaagaaatt gcccgtgcgg tagaccaagt ctccacagaa atgttccgca aagagtacgc
     3901 agaagttttt gaaggcacag ccgagtggaa ggaaattaac gtcacacgat ccgataccta
     3961 cggttggcag gaggattcaa cctatattcg cttatcgcct ttctttgatg aaatgcaggc
     4021 aacaccagca cctgtggaag atattcacgg tgcgcggatc ctcgcaatgc tgggggattc
     4081 agtcaccact gaccatatct ctccggcggg cagtattaag cccgacagcc cggcgggtcg
     4141 atatctacaa ggtcggggtg ttgagcggaa agactttaac tcctatggtt cgcggcgtgg
     4201 taaccatgaa gtgatgatgc gcggcacctt cgccaatatt cgcatccgta atgaaatggt
     4261 gcctggcgtt gaagggggga tgacgcgcca tttacctgac agcgatgtcg tctctattta
     4321 tgatgccgca atgcgctata agcaggagca aacgccgctg gcggtgattg ccgggaaaga
     4381 gtatggatca ggctccagcc gtgactgggc ggcaaaaggt ccgcgcctgc ttggtattcg
     4441 tgttgtgatt gccgaatcgt ttgaacgaat tcaccgttcg aatttaattg gcatgggcat
     4501 cctgccgctg gaatttccac aaggtgtgac gcgtaaaacg ttagagctta ccggggaaga
     4561 gaagattgat attggtgatc tgcaaaatct acaacccggc gcgacggttc cggtgacgct
     4621 tacgcgcgcg gatggtagcc aggaagtcgt accctgccgt tgtcgtatcg acaccgcgac
     4681 ggagttgacc tactaccaga acgacggcat tttgcattat gtcattcgta atatgttgaa
     4741 gtaacgagta tttgcttgcc ggttattttg cttccggcaa gcaaatcaat tacacaatgc
     4801 aagagggtta tttgttcagc aaatgaccca ttttctcggc tttggtatcg agataatgtt
     4861 cgttattggg gttacgacct acaatcaacg gtacgcgttc aataatatta atccctgctt
     4921 cggtcagaat ttcgactttt ttcgggttat tggttaacaa gcggacttca ttgacgccaa
     4981 ggagtttgaa catatcagcg caaagagtga agtcgcgctc atcagcggcg aagcctaact
     5041 ggtggttagc ctctacagta tcgtaacctt gatcctgcag tgcgtaagcg cggattttat
     5101 tcagcagacc aatgttacga ccttcctgac ggtgatacag caaaatacca cggccttcct
     5161 cggcaatttg cgtcaatgcc gcttcgagct ggaagccaca atcgcagcgc aagctgaaca
     5221 gggcgtcacc ggtcagacat tcggaatgga cgcgcgcaag taccggggta tgcccggaaa
     5281 tatcgccata gactagcgcg acatgatcgt gtccggttgc cagttcttca aatcccacca
     5341 tcaggaaatc gccccatggg gttggcagtt tggcttctgc cacacgttta agctgcatga
     5401 aattctccag ataatgctgg ttctgtattg gcttattttg ccataacgag aaggggttca
     5461 cctaatcacg acgcgtcgat cgttcacgga atggcacaaa tctgtcaatt tttcctggaa
     5521 ctggcatttt cagttatgat tgtgggactt atcaaaaagg agaggccatg cgttcgattg
     5581 ccagacgtac cgcagtggga gctgcactat tgcttgtcat gccagtagcc gtatggcttt
     5641 ctggctggcg ttggcaacct ggagaacaaa gttggttact aaaagcggct ttttgggtta
     5701 ctgaaactgt cacccagccc tggggcgtaa ttacacattt gattttattc ggctggtttc
     5761 tctggtgtct gcgttttcgc attaaggctg ccattatgtt atttgccatt ctggcggcgg
     5821 caatccttat gggacaaggc gttaaatcct ggatcaaaga caaagtccag gaaccacgac
     5881 cttttgttat ctggctggaa aaaacacatc atattccggt tgatgagttc tacactttaa
     5941 agcgagcaga acgcggaaat ctggtgaaag aacagttggc tgaagagaaa aatatcccac
     6001 aatatttgcg ttcacactgg cagaaagaga cggggtttgc ctttccttcc ggtcacacga
     6061 tgtttgctgc cagttgggca ctgctggccg ttggtttgct gtggccgcgt cggcgaacgt
     6121 taaccattgc tatcttgttg gtctgggcaa cgggagtcat gggaagccgc ctgctgctcg
     6181 ggatgcattg gccacgcgat ctggttgtag ctacgttgat ttcgtgggcg ctggtggcgg
     6241 tggcaacgtg gcttgcgcaa cgaatttgtg ggccattaac accacctgcg gaagaaaatc
     6301 gcgaaattgc acaacgagaa caagaaagtt aacgctggtt gattttccga atttagccct
     6361 taaatcatca acaatgcgcg tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa
     6421 agggctattg cggtaagttg accataattt attcgctcta accacataac gggaagtaat
     6481 gtgaaatatt tactcatttt cttactggtg ttagcgatct tcgtgatttc ggtcacgttg
     6541 ggtgcgcaga acgatcaaca ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt
     6601 atttccacat tgctggcggt attgtttgct gcggggtttg ctatcggttg gttgatttgt
     6661 ggcctgttct ggctgcgagt tcgtgtttcc ctggcgcgcg ctgaacgtaa aataaagcga
     6721 ctggaaaacc agctttcacc cgcgactgac gtggctgtag cgccgcactc gtctgcgtcg
     6781 aaggaataac tttctatgct ggagttgttg tttctgcttt tgcctgtagc cgctgcctat
     6841 ggctggtata tgggccgcag aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg
     6901 tcgcgtgatt acgtagcggg ggttaacttc ctgcttagta atcaacagga taaagcggta
     6961 gacctgtttc tcgatatgct taaagaggat acaggtaccg ttgaagccca ccttacgctc
     7021 ggaaacctgt tccgttcgcg tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta
     7081 atggaaagcg cctcgctgac ctatgaacag cggcttttgg cgattcaaca actggggcgt
     7141 gattacatgg ctgccgggtt atacgaccgc gcggaagaca tgttcaatca gctgaccgat
     7201 gaaactgact tccgcattgg cgcgctgcaa caactgctac aaatttacca ggctaccagc
     7261 gagtggcaga aagcaattga tgttgccgaa cgcctggtga agctgggtaa agataaacag
     7321 cgcgtcgaaa ttgcccattt ctactgtgag ttagctctgc agcatatggc cagcgacgat
     7381 ctcgatcgtg ccatgacttt gctaaaaaaa ggtgcggcgg cagataaaaa cagcgcccgc
     7441 gtatccatca tgatgggacg cgtgtttatg gcgaaaggag aatacgccaa agccgtcgaa
     7501 agtttgcaac gtgtcatatc ccaggacaga gaactggtca gcgaaacgct ggaaatgctg
     7561 caaacatgct atcagcagtt gggtaaaact gccgaatggg cagaattcct gcagcgcgcg
     7621 gtggaagaga acaccggtgc cgatgctgaa ttgatgcttg cggatatcat cgaagcgcgc
     7681 gacggtagtg aggccgcaca ggtctatatt actcgccagc ttcagcgtca tccgaccatg
     7741 cgtgtgttcc ataagttaat ggattaccac ttaaatgaag cagaagaagg gcgtgccaaa
     7801 gagagtctga tggtgctgcg tgacatggtt ggcgagaagg tacgtagtaa gccccgttat
     7861 cgctgccaga aatgtggttt taccgcatac accctctact ggcattgtcc gtcttgtcgg
     7921 gcctggtcaa ccattaaacc aattcgcggt cttgatggcc tgtaattttt aaaaaaatcc
     7981 gactttagtt acaacatact aattattgat gttctattgt gctccggcac cgacggcgcg
     8041 gaaaagcctg ccagaggaga aatcgcaact gttaattttt tatttccacg ggtagaatgc
     8101 tcgccgttta cctgtttcgc gccacttccg gtgcccatca tcaagaaggt ctggtcatga
     8161 cgttaactgc ttcatcttct tcccgcgctg ttacgaattc tcctgtagtt gttgcccttg
     8221 attatcataa tcgcgatgcc gcgatggcct ttgtcgacaa gatcgaccca cgcgattgtc
     8281 gtctgaaggt cggcaaagag atgtttacat tgtttgggcc acagtttgtg agcgaacttc
     8341 aacagcgtgg ttttgatatc tttcttgacc tgaaattcca cgatattccc aacactgcag
     8401 cgcacgctgt cgctgctgcg gctgacttag gcgtgtggat ggtgaatgtt catgcctccg
     8461 gtggggcgcg tatgatggcc gcagcgcgtg aggcactggt tccgtttggc aaagatgcac
     8521 cgcttttgat tgctgtgaca gtgttgacca gcatggaagc cagcgacctg gccgatcttg
     8581 gcgtgacact gtcacctgca gattatgcag aacgtctggc ggcactgacg caaaaatgtg
     8641 gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg ctttaaacag gtattcggtc
     8701 aggagttcaa actggttacg ccgggcattc gtccgcaggg gagtgatgct ggtgaccagc
     8761 gccgcattat gacgccagaa caggcgttgt cggctggtgt tgattatatg gtgattggtc
     8821 gcccggtaac gcaatcggta gatccagcgc agacgctgaa agcgatcaac gcctctttac
     8881 agcggagtgc atgatgagtg attccaacag ccgtctggtc tactcaacgg aaaccggacg
     8941 tattgatgag ccaaaagcgg cccctgtacg ccctaaaggc gacggtgtgg tgcgtattca
     9001 gcgtcagacc agtggacgca aaggtaaggg cgtttgcctg attaccggtg tcgatctcga
     9061 tgatgccgaa ctgacgaaac tggcagctga actgaagaaa aaatgcggct gcggcggagc
     9121 agttaaagat ggaattattg aaattcaggg cgataagcgt gatttattaa aatcaatgct
     9181 tgaagcgaaa gggatgaagg taaaactcgc aggcggctaa cataaaaagc cacggatata
     9241 tccgtggctt tcgaatattt tactgtgtgt attattcagt tatttcgtac tgaacagaat
     9301 caagcgtatt ataattgcga gtcgataatc gacatttatt tacgattatt taccgacctg
     9361 gtgaccaata acaccaccga cggctgcacc acctaatgta cccaacgtac tgccatcggt
     9421 cagtactgca ccgcctaatg cccctgcacc tgcaccgatt gcggtgttgc ggtcccgttt
     9481 agaccagtta gaacaggcac tcagagacat tgccagggta attgccagaa cagccgcggt
     9541 catttttttg ctcgttacaa acataatact ctctcctgaa tttatgattc acggaagtaa
     9601 gctctctata actataatag ctaagaataa gccaggtgaa attattccgt gaaatccgct
     9661 cgagcgcagg gttatatcac gcaggtgata atgacttcct gttatatcgc tgataataat
     9721 tttatatctt gagagtgtta ataacaggta aatagtctta attatcaacc aggaatcatc
     9781 ttagagcgga tgatttgcca aactgcaaat catccgtaga gaagggaaat ggttaaatgt
     9841 caacgacgtg aacggtgagt ttactttgtt caagatgcat cagatcgctg gcgcgtattt
     9901 tcgaatcggt aatcacgcga ttgaatcgct caacggggcc gatggagtat gaatgtacag
     9961 caccaaattt cgagctgtca gtcaggacta tcgcctcgca ctctttttcc agcacggcat
    10021 tgaccacatc ggtacgcatc atatcgcgac cggtaaaccc agtttcaggt tgccagccat
    10081 caataccaat aaatgctttg ctgaaatgca cttgttggat gcactggcgt gtcaaagggc
    10141 caaccatact ttcgcttttt ttctggtaca cgccaccgag caaaatgact tcacaaggcg
    10201 cgtctttcag caaatgcgcg atgtagctgc tgaccgtgat gatagtgaca tttttcttct
    10261 gctcgcccag agtcctggcg agcagggcat tgctgctacc attttcgata aagatggttt
    10321 cgcccggttg aaccagtgac gcggcaaact cggcgagttc acgcttcagc gtatagttgc
    10381 tcatcatacg ggtttcgacg tcatcactat caagcgaaac tgcaaaacca tgtgcacgtc
    10441 ggaggtaact cagtttttcg agggtgttga gatcctggcg aatggtaact tcagaaactc
    10501 cagtggcttt tgccagatcg gttacgctaa cctgaccctg gtcaatgacc atctgtagaa
//
LOCUS       DADSZD010000148        10550 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.149, whole genome shotgun sequence.
ACCESSION   DADSZD010000148 DADSZD010000000
VERSION     DADSZD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.149"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            153..1520
                     /gene="dcuC"
                     /locus_tag="J1Z28_003517"
     CDS             153..1520
                     /gene="dcuC"
                     /locus_tag="J1Z28_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBA7718320.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1564..2061)
                     /gene="sspB"
                     /locus_tag="J1Z28_003518"
     CDS             complement(1564..2061)
                     /gene="sspB"
                     /locus_tag="J1Z28_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBA7718321.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2067..2705)
                     /gene="sspA"
                     /locus_tag="J1Z28_003519"
     CDS             complement(2067..2705)
                     /gene="sspA"
                     /locus_tag="J1Z28_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBA7718322.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3100..3492)
                     /gene="rpsI"
                     /locus_tag="J1Z28_003520"
     CDS             complement(3100..3492)
                     /gene="rpsI"
                     /locus_tag="J1Z28_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBA7718323.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3508..3936)
                     /gene="rplM"
                     /locus_tag="J1Z28_003521"
     CDS             complement(3508..3936)
                     /gene="rplM"
                     /locus_tag="J1Z28_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBA7718324.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4156..5283)
                     /locus_tag="J1Z28_003522"
     CDS             complement(4156..5283)
                     /locus_tag="J1Z28_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBA7718325.1"
                     /translation="MQSITPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDENRTQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5477..5875
                     /locus_tag="J1Z28_003523"
     CDS             5477..5875
                     /locus_tag="J1Z28_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBA7718326.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6029..7396
                     /gene="degQ"
                     /locus_tag="J1Z28_003524"
     CDS             6029..7396
                     /gene="degQ"
                     /locus_tag="J1Z28_003524"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBA7718327.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINANKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIISALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIIRGNESL
                     YLLMR"
     gene            7486..8553
                     /gene="degS"
                     /locus_tag="J1Z28_003525"
     CDS             7486..8553
                     /gene="degS"
                     /locus_tag="J1Z28_003525"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBA7718328.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8616..9554)
                     /gene="mdh"
                     /locus_tag="J1Z28_003526"
     CDS             complement(8616..9554)
                     /gene="mdh"
                     /locus_tag="J1Z28_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBA7718329.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            9989..10459
                     /gene="argR"
                     /locus_tag="J1Z28_003527"
     CDS             9989..10459
                     /gene="argR"
                     /locus_tag="J1Z28_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBA7718330.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT         2594 a         2689 c         2628 g         2639 t
ORIGIN      
        1 cataagcgca tattttttaa tgaaaaatca cctcacctac aattaaaaac acgacatccg
       61 caccataaat agccttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga
      121 ttgaccgttt tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg
      181 tattaattac gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg
      241 ccgcaggtat cttcctgatg atgtgcggtg tctggttagg gttcggtggt gtgctcgatc
      301 ccgccaaaag cagcggctac ttgatcgtcg atatttataa tgaaatcctg cgcatgctgt
      361 ccaaccgcat tgccggattg gggctgtcga ttatggcagt gggcggttat gcccgctata
      421 tggagcgcat aggggcaagt cgcgcgatgg tgagcttgtt aagccgcccg ttaaaactca
      481 ttcgctcgcc gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt
      541 ttattaccag cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg
      601 tgagtctggg agtaagtcgt ctctctgcgg tggcggttat cgcaaccacc atgtccattg
      661 agtgggggat tctggaaacg aactccattt ttgctgccca ggtcgcagga atgaaaattg
      721 ccacttactt cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga
      781 tctcccactt tttcgtgcaa cgcgctttcg acaaaaaaga taaaaatatc aatcacgaac
      841 aggcagagca aaaagctctc gataatgtcc cgccgctcta ttacgccatt ttaccggtga
      901 tgccgttaat cctgatgctc ggctcgctgt tccttgccca cgtcgggctg atgcagtcag
      961 aactgcatct ggtggtggtg atgttactga gtttgacggt gacgatgttt gttgagttct
     1021 tccgcaagca taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg
     1081 gtacgcagtt tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct
     1141 taacgacgat tggcactgtc gatgcggtta tcaggggggc ggagcattct ggtctgggcg
     1201 gtattggcgt gatgattatt atggcgctgg tcattgccat ttgtgccatt gtgatgggct
     1261 caggcaatgc gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac
     1321 atgtaccagc ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgctg
     1381 tttcgccgat tactgcggtg gtggtcgtta cgtcaggaat tgcaggcgtt tcgccttttg
     1441 cggtagtgaa gcggacagcg atccccatgg cagtcggttt cgtggtgaat atgattgcca
     1501 caatcacgct attttattaa gccattaaaa agacaaaaca ggccgccagg gcctgtttta
     1561 gtattacttc acaacgcgta atgccggtcg accaccgcgt ggtggctgcg gaggttcatc
     1621 gtcaggatga gtttcatcat cgtgatctgg cttgtcgcca tcgataaccg acataacggt
     1681 ttcgttgtct gccgatgcct cttcatcatt catgatgctg gcatcttcat cgtaggcagc
     1741 ttcaggctca aacatcgtgc ctgcgccatt ttcacgggcg tagatagcca gcacggcagc
     1801 cagcggcaca gaaacctgac gcggaatgcc accaaagcgc gcgttaaagc gcacctcatc
     1861 attcgccagt tccagattgc cgacagcacg cggcgcaatg ttgagtacga tttgcccgtc
     1921 acgcgcatat tccataggaa cctgcacgcc tgggagcgtc acatccacca ccaggtgcgg
     1981 cgtgagctgg ttatccagca accactcata gaatgcacgc agcagatagg gacgacgtgg
     2041 tgttagctgt gacaaatcca tacagattaa ctccggccca gacgcatttc acgttctgct
     2101 tcagttaaag aagcaaggaa agagtcacgt tcaaagacgc gggtcatata gcctttcagc
     2161 tctttcgcac ccgggccgct gaactcgatg cccagttgcg gcagacgcca cagcagagga
     2221 gcaaggtagc aatcgaccag gctgaactca tcgctcagga agtacggctt ctgaccgaag
     2281 accggcgcaa tcgccagcag ttcttcgcgc agttgcttac gtgcggcatc tgcttcagaa
     2341 gctgaaccgt tgatgatggt gttcatcagc gtgtaccagt ctttttcgat gcgatgcatg
     2401 tacagacggc tttcaccgcg agctaccggg taaacaggca tcagtggcgg atgcgggaaa
     2461 cgctcatcca gatattccat aatgatgcga gattcccaca gggtcagctc acgatccacc
     2521 agggtcggaa cgctctgatt cgggttgagg tcaatcagat cctgaggcgg attgtccttt
     2581 tccacgtgtt cgatctcgaa acttacacct ttctcagcca gcacaatgcg gacctgatgg
     2641 ctatagatgt cagtaggacc ggaaaacagc gtcattaccg aacgtttgtt ggcagcgaca
     2701 gccatgaaaa cctccaggta tagtcagaat ttttactgct accagccacc aggtggccag
     2761 tcagaagttg tgttacccag taaggaacga ctctctttgt tcgaaaatca aacaaaaaat
     2821 gagcaatacc cgacatttgg gcagaaaatt ggatgatagt ttaccagatt ttgcgacctt
     2881 tgtggtgagt cgatgccgga aatggggaaa aagagatgcg ctttagtctg aaatagttga
     2941 cttagtccct tattggcgat gtggtttttg ttttacctgt ctgtcaggtg gcagcaaaaa
     3001 gcaactttcc attttttacg ctgatgcaga ttttagccat aaaaaaaccc gccgaagcgg
     3061 gttttttcgc aaattgtttt ctgccggagc agaagccaat taacgtttgg agaactgcgg
     3121 acgacgacgt gctttacgca gaccgacttt cttacgttca acctgacgag cgtcacgagt
     3181 aacgaagcca gctttacgca gttcagaacg cagggactcg tcgtattcca tcagagcgcg
     3241 ggtgataccg tgacggatcg caccagcctg accagagata ccaccacctt taacggtgat
     3301 gtacaggtcc agtttctcaa ccatgtcgac cagttccagc ggctgacgaa ctaccatgcg
     3361 agcagtttca cgaccgaagt actgttccag agaacgttgg ttgattacga ttttaccgtt
     3421 gcccggtttg atgaaaacgc gagctgcgga acttttgcgg cgaccagtgc cgtagtattg
     3481 attttcagcc attgcctata atcccgatta gatgtcaaga acttgcggtt gctgtgccgc
     3541 gtggttgtgc tcgttacccg cgtaaacttt cagtttacgg aacatagcac gacccagcgg
     3601 gccttttggc aacatgcctt taaccgcgat ttcaatcaca cgctcaggac ggcgagcaat
     3661 catctcttca aaggtcgctt gtttgatacc accgatgtgg ccggtgtggt gatagtacac
     3721 tttgtcagta cgcttgttgc cggttacagc aactttgtca gcgttcagaa cgatgatgta
     3781 atcaccggta tctacgtgcg gagtgtattc cgctttgtgc ttaccgcgca ggcgacgagc
     3841 cagttcagta gccagacggc ccagagtttt accggtcgcg tcaacaacat accagtcgcg
     3901 ttttacggtt tctggtttag ctgtaaaagt tttcattaaa agcttaccca ataaatagtt
     3961 acacgttggt gaacacccaa acgtcttcaa ttgttgaggt tcacacgaca aagtccggca
     4021 aacctacccc ttcgaatagc ctatgccagc acacaaaaag tttcgggaaa aaaaactttc
     4081 ttgtaacgtg gggtcgcagg attatagaga agtcggggtc aaagatcgac ccctttttgt
     4141 gatttgtggc aggttttacc ccgccaaatg ctcgcgcttc aaatactctt cgctttgcat
     4201 ctcttgcaga cgtgacaggc aacgctggaa ctcaaacttc agccgctcgc cctgataaat
     4261 ttcatacagc ggcacttctg cactcaccac taatttgaca tggcgctcgt aaaactcatc
     4321 caccagcgca ataaagcgcc gcgcttcgct ctccatcaac cgcgtcataa ctggtacatc
     4381 aaacaacatg accgtgtgaa agaggcgtga gagcgcaata tagtcatgct gactgcgggc
     4441 atcgacgcac aaggtagtaa aagaaaccgc cagcgtctgg ttctcgacgc ccattgtcgc
     4501 caatggccga tggttgattt ctaacgtcgg tgaattttct cgtttcgccc ccgccagcgc
     4561 caaccatagt ttatccattt gcgtccggtt ttcatcgttg agtggcgaaa gccacagatg
     4621 cgcctgagtg agtgtacgca gtcgataatc aacaccagcg tccacgttca ttacatcaca
     4681 atgctgttta atggcatcga ttgcaggcaa aaaacgcgca cgttgcaggc catttcgata
     4741 aagttcgtcc ggcggaatat ttgacgttgc taccagggta ataccgcggg cgaacagggc
     4801 tttcatcaga ccgccaagta gcatggcatc ggtaatatca gaaacaaaaa attcgtcaaa
     4861 acagagcacg tcagtttctg ctttaaagcg atcggcaata atttccagcg gatcggtctg
     4921 cccctgcaaa gcagttagct cttcatgtac ccgcagcata aaacggtgaa agtgcagacg
     4981 ctgtttccgc tctcccggca ggctttgata gaaaaggtcc atcagccagg ttttcccgcg
     5041 tcctacaccg ccccacatat ataagccacg cactggcgta tgctttgtgt cttcgcgttt
     5101 accccacagc ttaccgaccc gcgccattag cccactcgtc ctgggggctg gaggcgtgct
     5161 attgatgagt tcctgataaa taatttccag gcggctgacg gcctcttttt gaacgtcgtc
     5221 gggttgatgg ctgccttcat tgagcgcctt caggtattgc gatgttgggg taatgctttg
     5281 catgatctta ttgttattcc ttgaataatc ggtgcgccgt tgttcacggt tgacgaaaaa
     5341 aaggccgttc tacactacgc gatatgcagt cgggattcca cttctgtgga attaacggtt
     5401 atagtggcat aatcagccgc aggcatggag cctgaagcca acaccctacg gaaacaaaag
     5461 acaacgggag atgttcatga cctgggaata tgcgctaatt gggttagtcg tcggcatcat
     5521 tattggtgct gtggccatgc gttttggtaa tcgtaaacta cgccagcaac aggcgttgca
     5581 gtacgaactg gaaaagaata aagctgaact ggacgagtat cgcgaagagc tggttagcca
     5641 ctttgcccgc agcgcggaat tactggatac catggcgcac gactatcgcc agctgtatca
     5701 gcacatggcg aaaagctcca gtagcctgct gccggaactg tctgcggaag caaacccgtt
     5761 ccgtaatcgt ctggcagagt ctgaagccag caacgatcag gcaccggtgc agatgcctcg
     5821 cgactattct gaaggcgcat ccggcctgct gcgtactggc gcgaagcgcg actaatttat
     5881 ttttcgggcg cagccattgc gccctcctct tctctccctc cccgactatc atttaatctg
     5941 gtgtctcatt gttagccgtc tgaaaattca ataacatcaa actgttttga atctcttttc
     6001 ttatcattca ggtacgagag caggaataat gaaaaaacaa acccagctgt tgagtgcatt
     6061 agcgttaagt gtcgggttaa ctctctcggc gtcatttcag gccgtcgcgt cgattccagg
     6121 ccaggttgcc gatcaggccc ccctccccag tctggctcca atgctggaaa aagtgcttcc
     6181 ggcagtggtg agcgtacggg tggaaggaac ggccagtcag ggacagaaaa tcccggaaga
     6241 attcaaaaag ttttttggtg atgatttacc ggatcaacct gcacaaccct tcgaaggttt
     6301 aggctccggt gtcatcatca acgccaataa aggctatgtg ctgaccaaca accatgtgat
     6361 taatcaggca cagaaaatca gtattcagct caatgatggg cgcgagtttg atgcaaaact
     6421 gattggtagc gatgaccaga gcgatatcgc cctgttgcaa attcaaaacc cgagcaaatt
     6481 aacgcaaatc gctattgccg actccgataa attgcgcgtc ggtgattttg ccgtggcggt
     6541 cggtaaccca tttggccttg ggcaaaccgc cacctctggc attatatccg cattaggccg
     6601 cagcgggttg aatcttgaag gtctggaaaa ctttatccag acagatgctt ccattaaccg
     6661 cggtaactcc ggcggtgcac tgttaaacct taacggtgag ttaattggca tcaacactgc
     6721 aatccttgcg cctggcggcg gtagcgtcgg gattggattt gccattccca gtaatatggc
     6781 gcgaacactg gcgcagcagc ttatcgactt tggagaaatc aaacgcggcc tgttaggcat
     6841 caaaggcacc gagatgagtg ccgatatcgc caaagccttc aaccttgacg tgcagcgtgg
     6901 cgcgtttgtc agcgaagtgt tgccaggttc tggctcggcg aaagcgggcg tcaaagcggg
     6961 cgatattatt accagcctca acggcaaacc gctgaatagc tttgctgagt tgcgctctcg
     7021 tatcgcgacc accgagccgg gcacaaaagt gaagcttggc ctgctgcgta acggcaaacc
     7081 actggaagta gaagtgacgc tcgataccag cacctcttcg tcggccagcg ctgaaatgat
     7141 cacgccagcg ctggaaggtg caacgttgag cgatggtcag ctaaaagatg gtggcaaagg
     7201 tattaagatc gatgaggttg tcaaaggaag cccagctgct caggctggct tgcaaaaaga
     7261 cgatgtgatc attggcgtca accgcgatcg ggtgaactcg attgctgaaa tgcgtaaagt
     7321 gctggcggca aaaccggcca taatcgcctt gcaaatcata cgcggcaatg aaagcctcta
     7381 cctactaatg cgttaatgtc gtaaaccggg catcaggctg acgtgtgatg tccggttaac
     7441 tcgtggtatg ctgctgccgt tccctttttt aacgatgcct ccatcatgtt tgtgaagctc
     7501 ttacgttccg ttgcgattgg attaattgtc ggcgctattc tgctggttgc catgccttcg
     7561 ctgcgcagcc ttaacccgct ttccactccg caatttgaca gtaccgatga gacgcctgcc
     7621 agctataatc tggcggttcg ccgcgccgcg ccagcggtgg ttaacgttta taaccgcggt
     7681 ttgaacacca actctcacaa ccagcttgag atccgcaccc tgggatctgg tgtaatcatg
     7741 gatcaacgcg ggtatatcat caccaataaa cacgtgatca acgacgccga tcagatcatc
     7801 gtcgccttac aggatggacg tgtttttgaa gcgttactgg ttggctctga ctctctaacc
     7861 gatctggcgg tgctgaaaat taacgccaca ggcggtttac caacgattcc gatcaatcca
     7921 cgccgcgtac cgcacattgg cgatgtggtt ctggcgatcg gtaacccgta caacctcggg
     7981 cagaccatta cccaggggat tatcagtgcc acgggtcgaa tcggtctgaa cccgaccggg
     8041 cggcaaaact tcctgcaaac cgatgcttcc attaaccacg gtaactctgg cggcgcgctg
     8101 gtgaactcgc tgggcgaact gatgggcatt aacacgctgt cgtttgataa gagtaacgat
     8161 ggcgaaacgc cggaaggtat cggctttgcg attcctttcc agttagcgac caaaattatg
     8221 gataaactga tccgtgatgg ccgtgtgatc cgtggctaca ttggcattgg tggacgtgag
     8281 atcgccccat tacatgcgca gagtggcggc atggatcaac tgcaagggat cgtggttaac
     8341 gaagtgtcgc ctgatggccc agcggcaaat gcaggcatcc aggtcaacga tctgattatc
     8401 tctgtgaata acaaacccgc tatttccgcc ctggagacaa tggatcaggt cgcggaaatt
     8461 cgccctggtt cggtgatccc ggttgtcgtg atgcgtgacg ataaacagtt aacattgcag
     8521 gtcaccattc aggaatatcc ggcgaccaac taaggtcagc gtaaaaaaac aaaaaccgga
     8581 gtctgtgctc cggtttttta ttatccgcta atcaattact tattaacgaa ctcttcgccc
     8641 agggcgatat ctttcttcag cgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
     8701 ctcagagtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
     8761 gagaagaaac gagcgtactg accgtcgcct tcaacgtagg cacattcgac aacgccttgt
     8821 tcgccctgca gtgcgcgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
     8881 agggttgcag acccgccacc ggctttcgct tcaaccacct cagtacccgc gttctggata
     8941 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
     9001 agcggcagaa tggtaacacc agagtgacca ccaataaccg gcacttcaac ttcgcctggc
     9061 tgtttgcctt tcagttccgc aacaaaggtg ttggaacgaa tgatatccag cgtggtaacg
     9121 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
     9181 actgtagtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
     9241 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
     9301 atacccggtt tacgcgctac gcctgcagag ataagaacga catctgcacc ttccagcgcc
     9361 ggagtcgcat cttcacctga aaaacctttg attttcacag cagtagggat atggctcaga
     9421 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
     9481 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
     9541 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
     9601 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
     9661 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
     9721 aacagtcact tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgct
     9781 cagccaaaac aatatcaatt tgataacaat taatttactt ttaagcagaa tttgcaggcc
     9841 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
     9901 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
     9961 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
    10021 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgttgc
    10081 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
    10141 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
    10201 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtactgg atatcgacta
    10261 caacgatgca gttgtcgtga ttcataccag ccctggtgcg gcgcagttaa ttgctcgcct
    10321 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
    10381 ctttactacc cctgctaacg gtttcaccgt caaagagctg tacgaagcga ttttagagct
    10441 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
    10501 ttgcctatca accccctgct ttcccctgcg attcatttaa cgaatagtgc
//
LOCUS       DADSZD010000149        10427 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.150, whole genome shotgun sequence.
ACCESSION   DADSZD010000149 DADSZD010000000
VERSION     DADSZD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.150"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            107..2248
                     /locus_tag="J1Z28_003528"
     CDS             107..2248
                     /locus_tag="J1Z28_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HBA7718331.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYDLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESVGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(2312..4204)
                     /gene="parE"
                     /locus_tag="J1Z28_003529"
     CDS             complement(2312..4204)
                     /gene="parE"
                     /locus_tag="J1Z28_003529"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBA7718332.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSEDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(4233..4814)
                     /gene="yqiA"
                     /locus_tag="J1Z28_003530"
     CDS             complement(4233..4814)
                     /gene="yqiA"
                     /locus_tag="J1Z28_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBA7718333.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(4814..5641)
                     /gene="cpdA"
                     /locus_tag="J1Z28_003531"
     CDS             complement(4814..5641)
                     /gene="cpdA"
                     /locus_tag="J1Z28_003531"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBA7718334.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPGRHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(5666..6088)
                     /locus_tag="J1Z28_003532"
     CDS             complement(5666..6088)
                     /locus_tag="J1Z28_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBA7718335.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLSRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(6089..6718)
                     /gene="nudF"
                     /locus_tag="J1Z28_003533"
     CDS             complement(6089..6718)
                     /gene="nudF"
                     /locus_tag="J1Z28_003533"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBA7718336.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            6923..8404
                     /gene="tolC"
                     /locus_tag="J1Z28_003534"
     CDS             6923..8404
                     /gene="tolC"
                     /locus_tag="J1Z28_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBA7718337.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGATAGQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            8552..9223
                     /locus_tag="J1Z28_003535"
     CDS             8552..9223
                     /locus_tag="J1Z28_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBA7718338.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPV
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            9229..10389
                     /locus_tag="J1Z28_003536"
     CDS             9229..10389
                     /locus_tag="J1Z28_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBA7718339.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIAAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         2627 a         2766 c         2588 g         2446 t
ORIGIN      
        1 tcaattacat atccgatggc gaaataacaa acggatagtg cttagtgagc aataagaaac
       61 aattttcact cgcgacaatc cgcgagtttt tgggataaca gactaaatgg ctatgttcac
      121 accttcattc tcaggactca aaggtcgggc gctcttttca ctgctttttg cggcaccgat
      181 gattcatgca acagactctg taacgaccaa agatggcgaa acaatcactg ttacagcaga
      241 tgcaaatacc gcaactgagg caaccgatgg ttatcaacct ctgagcacct ccacggcgac
      301 attaaccgat atgccgatgc tggatatccc gcaggtggtc aatacggtta gcgatcaggt
      361 tctggaaaac cagaatgcga caacgctgga tgaggcgctt tataacgtca gtaacgtggt
      421 acagaccaat acattaggcg ggactcagga tgcttttgta cgccgtgggt ttggcgcaaa
      481 ccgggatggc tccatcatga ccaacggtct gcgaaccgta cttcctcgta gtttcaacgc
      541 cgcaacagag cgtgtggaag tgctaaaagg cccggcctcc acgctgtatg gcattctcga
      601 tcctggcgga ctgattaacg tcgtgaccaa gcgcccggaa aaaacattcc atggttcggt
      661 ttcagccacc tcctccagtt ttggtggcgg cactgggcaa cttgatatca caggtcccat
      721 tgaaggcact cagctggcgt atcgtcttac cggggaagtg caggatgaag attactggcg
      781 aaacttcggt aaagagcgca gtacatttat tgccccgtca ctcacctggt ttggtgataa
      841 tgcaacagta accatgctct attcccatcg ggactataaa actccgttcg atcgtggaac
      901 gattttcgac cttacgacga aacagcccgt aaacgttgat cgaaaaatac gttttgacga
      961 accgtttaat attacagatg gtcagtccga tctggcgcaa ctcaacgcag aatatcatct
     1021 caatagccag tggacagcgc gctttgatta cagctacagc caggataaat acagcgataa
     1081 tcaggcgcgt gttaccgcgt atgatgcaac gacaggaaca ctgacacggc gtgttgatgc
     1141 aactcaggga tctacccagc gtatgcatgc tactcgtgcg gatctgcaag ggaatgttga
     1201 tattgccgga ttctataatg agattctggg tggggtgtca tatgaatatt atgatcttct
     1261 gcgtacagat atgattcgct gtaaaaaagc taaagatttc aatatataca accctgttta
     1321 tggtaatacc agcaaatgta caacggtttc ggcgtcggac agtgatcaga cgatcaaaca
     1381 ggagagctac tcagcgtatg cacaggacgc actctatctg accgataact ggattgccgt
     1441 cgccgggatc cgctatcagt attacacgca gtatgcgggt aaaggccgtc cttttaatgt
     1501 caatactgac agccgcgatg aacaatggac tcccaaactg gggttggtct acaaactgac
     1561 gccatcggta tccttatttg ccaattattc gcaaacattt atgccgcagt cgtcaattgc
     1621 cagctacatt ggcgatcttc caccagaatc atctaatgct tacgaagtcg gggcaaaatt
     1681 cgagctattc gatggtatca ccgcagatat tgcgctgttt gatatccata aacgtaatgt
     1741 gttgtatacc gaaagtgttg gtgatgaaac cattgccaaa acggcaggcc gtgttcgttc
     1801 aagaggggta gaagtcgacc ttgcgggagc attaactgaa aacattaata tcattgccag
     1861 ctacggctat accgatgcta aggttctgga ggatcctgat tatgcaggga aaccattgcc
     1921 gaatgttcct cgtcatactg gttcgctatt cctgacctat gatattcata acatgccagg
     1981 caataatacg ctgacatttg gcggtggtgg acatggtgta agccgtcgtt cggcaaccaa
     2041 tggggctgac tattatctgc caggctattt tgttgccgat gccttcgccg catacaaaat
     2101 gaaattgcag tatccggtca cactgcaatt aaacgtcaaa aacctgtttg ataaaacgta
     2161 ttacacctct tccatcgcca caaataatct ggggaatcag attggcgatc cgcgtgaagt
     2221 gcaattcacg gtgaaaatgg aattttgatc aaaaaagccc ggtatacact tgtataccgg
     2281 gctatgtttt attcttctgg cgggaggatt atcagacctc aatctctgcc atgtcgcctt
     2341 tctcttgcaa ccagttgcgg cgatcttccg agcgtttctt cgccagcagc atatccatca
     2401 tcgcgtcagt acgctgatcg tcttcatcat cgatagtcaa ctgcaccaga cggcgagtgt
     2461 tcggatcaag cgtggtttcg cgcaattgca tcgggttcat ttccccgaga cctttaaaac
     2521 gctggacgtt tggcttgcct ttcttgcgtt ttaattgctc aagtacgccc tctttctctt
     2581 cttccgtcag cgcgtaataa acctctttcc cgaggtcaat acggtagagc ggtggcagtg
     2641 caacgtaaac gtgaccgtgt ttcaccaatg cgcggaagtg ttttacaaac aaagcgcaga
     2701 gcagcgtggc aatgtgcaga ccatcggagt cagcatccgc caggatacag atcttgccgt
     2761 aacgaagctg gctcaaatct tcgctgtcag gatcgatacc gatagctacc gaaatatcgt
     2821 gcacttcctg cgaagccagc acttcgtcgg aagagacttc ccaggtatta aggatcttac
     2881 ctttcagtgg catgatcgcc tgatattcgc gatcgcgcgc ctgcttggca gatccgcctg
     2941 cggagtcacc ttccacaagg aatagttcgg tacggttaag atcctgcgcg gtacagtcag
     3001 ccaatttacc aggcagcgcc gggccgctgg ttagcttttt acgcaccact tttttagccg
     3061 cacgcatacg acgctgggcg ctggaaatcg ccatctctgc caacagctcc gccgcctgaa
     3121 cgttctggtt cagccacagg atgaaggcat ctttcaccac gcccgaaacg aatgccgcac
     3181 actgacgcga agagagacgt tctttggtct gcccggcaaa ctgcggatcc tgcattttta
     3241 ctgacagcac ataggcgcag cgatcccaga tatcttccgc cgacagcttt acaccgcgcg
     3301 gcagaatgtt gcggtattca cagaactcac gcatcgcgtc cagcaggccc tgacgcaggc
     3361 cattaacatg ggtaccgccc tgcatcgttg ggatcaggtt gacgtagctt tcggtcagca
     3421 gttcaccgcc ttccggcagc cacagtagcg cccagtccac cgcttcagta tcgccagcga
     3481 aattaccgat aaacggtttt tctggcagcg tcggtaaacc gtttaccgct tccgccaggt
     3541 aatcattcag accgtcctga tagcaccagc gctgttcggt gttgttgatc tcatctttaa
     3601 aagtgatctc aacgcccggg cacagtaccg ctttggcttt cagcacatgc gtcaggcgtg
     3661 aaacagaaaa acgcgggctg tcaaagaagg tttcatccgg ccagaagtgc acgctggtac
     3721 cggtattgcg tttaccgcaa gtgccgacaa cctgtaaatc ttgcaccttt tcgccatttt
     3781 caaaggcgat gttatagatc tgaccatcgc ggcgtacgtt aacttctacg cgcttcgaca
     3841 gggcgttaac taccgaaatc cccacgccat gcaggccgcc agagaactgg taatttttgt
     3901 tagagaattt accgcccgcg tgcagacggc aaagaatcag ttcaaccgcc ggtaccccct
     3961 cttccgggtg aatatctacc ggcatcccgc gcccatcgtc aatcacttct aacgactggt
     4021 cggcatgtaa gattacatcc acgcgttttg cgtggcccgc cagtgcttca tcgacactgt
     4081 tatcaataac ttcttgcccc aaatggttag ggcgagtggt atcggtatac atccccggac
     4141 ggcggcgaac cggctcaagc ccggtgagta cctcaatggc atcagcgtta taagtttgcg
     4201 tcatggttta agttagcaat tcgagttgat cgtcagagat ggtgcagacc aagaaaatcg
     4261 acgatcgggt tgaaataatc ttcgaagccc gtgaatgcgt ggttgccgcc ttctatgaca
     4321 gtctggcggc aggaagcgta gtacgccacc gcctggcggt aatccagcac ttcatctccc
     4381 gtctgttgca gcaaccagat caaatccggc gcttccagcg ggtcaatctg catgactttg
     4441 agatcgtaaa tatggcgtga ctctagcaca tattgctgcc cggtgtaggg gttctcgttc
     4501 tgaccgagat agtccgtcag cagttcaaac gggcgcaccg ccgggttgac caccactgcg
     4561 ggcagcataa aacattgcga caaccaggtg gcgtaatatc cccccagtga cgaaccgaca
     4621 atacccagcg aatcgccgcc atgttccagg acaatggatt ccagcagttc tgccgcgtca
     4681 gaaggatacg gcggcaactg cggaatgatc atctcaacgt cagggtgatg ttccgccagc
     4741 cagtttttta acaagctcgc ttttgcagag cgcggcgagc tgttgaaacc gtgtaaataa
     4801 agaagcgtag acatcagtag ccttctgaag cggtatcagg ttggaagcgt gcgtccgcca
     4861 ggcgatgcac ctcggtggtc agcgtgccat cagcatgtaa ctcgagagta cgccagccgg
     4921 gcgcgatggt gtccagcgta aagttagaac agtgcggctt aaactgcaca caggtcgacg
     4981 gcgttgccag caagcggcga ccattccagt cgagatccag ctcctgatga atgtgaccgc
     5041 acagcaagta tttgacgtgc ggaaacttcg ccagcacggt atccagttcg cccgcgttac
     5101 gcaggctgtg ttgatcaagc caactacaac ctgcaggtag cggatgatga tgcagcagca
     5161 gcaacgtatg gcgtcctggc gcatcggcca gtttacgttc cagccactca agctgaaact
     5221 cgctcagctc accgtgcggc acgccaaaca cctggctatc cagcaacagg atttgccatt
     5281 gctcaccaat aaacacgcgc ttcgccgggg agatacccgc atcctgtaac gcactgtaca
     5341 tcgcgggctg gaaatcgtgg ttgcccggca gccagacgca gggcgcacga aaacttgcga
     5401 tgccttcagc gaaatgctga taggccgcag aggattgatc ctgcgctaaa tcacctgtcg
     5461 cgacaatcag gtcgaattcg tgctggtgtg gccgaatcgc ctccagcacc gcctggtagc
     5521 tttcccaggt gtttacccct aacagggctt cgtgcttttg tgcaaacagg tgagtgtcgg
     5581 taatttgtaa aatcctgact ctggcctcac cagccagagg aagggttaac aggctttcca
     5641 aatggtgtcc ttaggtttca cgacgctaat aaaccggaat cgccatcgct ccatgtgcta
     5701 aacagtatcg caaccagtcc gctaaaaact gattaatttg atgcttttcg tcgcgttgat
     5761 gcaacttttt attaggataa tcataccgcg ctttgaagcg aaaaatctgc tggcttgaac
     5821 acacttcagc caccatcgcg tcatgataca gacgcaccgt cattgacgga aggctccagt
     5881 aactgatcgc gggcgcagtc tgttctattg tcaccagggt agtgtatcgg gtcgattcca
     5941 caatcgtcag ccgatattgt gcgtttgcca cctgatagct tacagtttcg ccgggtgcgt
     6001 cattgcgcga taacaaacgg cgcaattgtg aaaagttcat ctcgcacagg cgcatcattt
     6061 caggaaagtc aggtgtgtaa cgcttcattt atgcccactc attttttaac gcttgatgat
     6121 gcagctgcag ccattgcaaa gcgatgaccg acgctgcgtt gtcgattttc ccctcttcta
     6181 cccactggta tgcctgttcc cggcttacca catgaacgcg aatatcttcg ttttcatcag
     6241 ccagaccgtg aataccgctt gcggtcgtgg cgtccacttc gcccaccata attgacgaac
     6301 gctcactggt gccccccggg cttgccagga aacttaacac cggtttggtc cgtttgacta
     6361 tcagtcccgc ctcttcaatc gcttcgcgac gggcaacatc ttccacactt tcaccctctt
     6421 caatcatccc ggcaaccatc tccagtagcc agggggtttc gctggtgtcg tacgcggcaa
     6481 tccgaatctg ctcaatcagc acaacttcat cacgtactgg gtcaaagggt agcaagactg
     6541 cggcgtgacc gcgctcaaaa atttcccgcc gtacctcatg actcatttgc ccgttgaata
     6601 gacgatgacg aaatctataa agatctaatg aaaaaaagcc gcgataaagt gtttctcgtg
     6661 caataatttc tacatcgttt ttgccaaatg taacgggcag gttgtctggc ttaagcattg
     6721 ttaatgtcct ggcactaata gtgaattaaa tgtgaatttc agcgacgttt gactgccgtt
     6781 tgagcagtca tgtgttaaat tgaggcacat taacgcccta tggcacgtaa cgccaacctt
     6841 ttgcggtagc ggcttctgct agaatccgca ataattttac agtttgatcg cgctaaatac
     6901 tgcttcacca caaggaatgc aaatgaagaa attgctcccc attcttatcg gcctgagcct
     6961 ctctgggttc agttcgttga gccaggccga gaacctgatg caagtttatc agcaagcacg
     7021 ccttagtaac ccggaattgc gtaagtctgc cgccgatcgt gatgctgcct ttgaaaaaat
     7081 taatgaagcg cgcagtccat tactgccaca actaggttta ggtgcagatt acacctatag
     7141 caacggctac cgcgacgcga acggcatcaa ctctaacgcg accagtgcgt ccctgcagtt
     7201 aactcaatcc atttttgata tgtcgaaatg gcgtgcgtta acgctgcagg aaaaagcagc
     7261 tgggattcag gacgtcacgt atcagaccga tcaacaaacc ttgatcctca acaccgcgac
     7321 cgcttatttc aacgtgttga atgcgattga cgttctttcc tatacacagg cgcaaaaaga
     7381 agcgatctac cgtcaattag atcaaaccac ccaacgtttt aacgtgggcc tggtagcgat
     7441 caccgacgtg cagaacgccc gcgcacagta cgataccgtg ctggcgaacg aagtgaccgc
     7501 acgtaataac cttgataacg cggtagagca gctgcgccag atcaccggta actactatcc
     7561 ggaactggcg gcgctgaatg tcgaaaactt taaaaccgac aaaccacagc cggttaacac
     7621 gctgctgaaa gaagccgaaa aacgtaacct gtcgttgtta caggcgcgct tgagccagga
     7681 cctggcgcgc gagcaaattc gccaggcgca ggacggccac ttaccgactc tggatttgac
     7741 ggcctctacc ggaatttctg acacctctta cagcggttca aaaacccgtg gtgcgaccgc
     7801 aggccagtat gacgatagca atatgggcca gaacaaagtt ggcctgagct tctcgctacc
     7861 gatttatcag ggcggaatgg ttaactcgca ggtgaaacag gcccagtaca actttgttgg
     7921 tgccagcgag caactggaaa gcgcgcatcg tagcgtcgtg caaaccgtac gttcctcctt
     7981 caacaacatt aatgcttcta tcagtagcat taacgcctac aaacaagccg tagtttccgc
     8041 tcaaagctca ttagacgcga tggaagcggg ttactcggtt ggtacgcgta ccattgttga
     8101 tgtgttggat gcgaccacca cgctgtacaa cgctaagcaa gagctggcaa atgcgcgtta
     8161 taactacctg attaaccagt tgaatattaa gtcagctctg ggtacgttga acgagcagga
     8221 tctgctggca ctgaacaatg cgctgagcaa accggtttcc actaatccgg aaaacgttgc
     8281 cccgcaaacg ccggaacaga atgctattgc tgatggttat gcgcctgata gcccggcacc
     8341 agtcgttcag caaacatccg cacgcactac caccagtaac ggtcataacc ctttccgtaa
     8401 ctgatgacga cgacggggct tcggccccgt ctgaacgtaa ggcaacgtaa agatacgggt
     8461 tatctgccgc attcttcccc cttctcgctt caatttcgac cagccatcct ctattctgat
     8521 gggtatttac cactggtccc ggaagacaaa aatgaaacgg acaaaatcca tacgccacgc
     8581 atcgttccgc aaaaactgga gcgcacgcca tctgacacca gtcgctctcg cggttgccac
     8641 tgtttttatg ctggctggct gtgaaaagag tgatgaaaca gtgtctctct atcaaaatgc
     8701 tgacgactgt tcagctgcaa acccaggcaa aagcgccgaa tgtaccaccg cgtacaacaa
     8761 tgcgctgaaa gaagccgaac gtactgcgcc gaaatacgcc acccgtgaag actgtgttgc
     8821 tgaatttggt gaaggtcagt gccagcaggc accagtccag gctggcatgg caccagaaaa
     8881 ccaggcgcag gcccagcaat ccagcgggag tttctggatg ccgctgatgg ccggttacat
     8941 gatgggacgt ctgatgggcg gcggcgcggg atttgcacag cagccgctgt tctcctcgaa
     9001 aaacccggcc agcccggctt acggtaaata taccgacgcg accggtaaaa actatggcgc
     9061 agcccagcca ggccgcacca tgaccgtacc gaagacggca atggcaccaa aaccggcgac
     9121 cacaactact gttacccgtg gcggttttgg tgaatctgtt gccaaacaaa gcactatgca
     9181 gcgtagcgca accggcacct cttctcgttc aatgggtggc tgataccgat ggaaagagtc
     9241 agtattaccg agcgcccgga ctggcgcgaa aaagcccacg aatacggttt caattttcac
     9301 accatgtacg gcgagccgta ctggtgtgaa gatgcttact acaagttgac cctcgctcag
     9361 gttgaaaagc tggaagaagt caccgccgaa ctgcaccaaa tgtgcctgaa agtggtggaa
     9421 aaagtgatcg ccagcgatga gctgatgacc aaattccgca taccaaaaca cacctggagt
     9481 ttcgtgcgcc agtcatggct gacgcaccag ccatcgcttt attcgcgtct tgatctggcg
     9541 tgggatggca ctggcgaacc taagcttctg gaaaataacg ccgatacgcc aacgtcacta
     9601 tacgaggcgg cgttctttca gtggatctgg ctggaagatc agcttaacgc cggtaacttg
     9661 ccggagggca gcgaccagtt taacagtttg caagaaaagc tgatcgatcg cttcgttgag
     9721 ctgcgtgaac agtatggctt ccagttgctg catctcacct gctgtcgcga cacggtggaa
     9781 gatcgcggaa ccattcagta tttgcaggac tgcgcgacgg aagctgaaat cgctaccgag
     9841 ttcctctaca tcgacgatat cgggttaggt gaaaaaggtc agttcacgga tttacaggat
     9901 caggtgattt ccaacctgtt caagctgtat ccgtgggaat ttatgttgcg tgagatgttc
     9961 tccaccaagc tggaggatgc aggcgtacgc tggctggaac cggcgtggaa gagcattatc
    10021 tccaacaaag cgcttctacc gctactgtgg gagatgttcc cgaatcaccc gaacctgctg
    10081 cccgcttatt ttgcggaaga tgatcatccg caaatggaaa aatatgtggt taaaccgatc
    10141 ttctcccgtg aaggcgcaaa cgtgtcgatc attgagaacg gcaaaaccat tgcagcagcg
    10201 gaaggtccgt atggcgaaga agggatgatt gttcagcaat tccacccgtt accgaaattc
    10261 ggcgacagct atatgctgat tggtagctgg ctggtgaacg atcaacctgc cggaattggc
    10321 attcgtgaag accgtgcatt gatcacccag gatatgtctc ggttttatcc gcatattttt
    10381 gttgagtaat acagcttaat attgccccga taagcggggc aatctct
//
LOCUS       DADSZD010000150        10388 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.151, whole genome shotgun sequence.
ACCESSION   DADSZD010000150 DADSZD010000000
VERSION     DADSZD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.151"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            5..2647
                     /gene="tssH"
                     /locus_tag="J1Z28_003537"
     CDS             5..2647
                     /gene="tssH"
                     /locus_tag="J1Z28_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HBA7718340.1"
                     /translation="MDTPVSRSALYGKLTKSLFRSLESATTFCKLRANPYVELTHWLH
                     QLIQQPENDIHNVLRHYQITSADVEKALIQQLNILPAGASVINDFSHHIDLSVEKAWM
                     LASIRFGDQKIRSGWLLLAWLTTPELYRVLKNICAPLAGLPVDELLDILPSLIASSPE
                     LTEIPYDGSDLNFAVPGEASQVFAGESTGSKSALAKYCQDMTALAREGNIDPVTGREH
                     EIRTMTDILLRRRQNNPLLTGEAGVGKTAVVEGFALAIAGGEVPPSLRNVRLLSLDVG
                     ALLAGASMKGEFESRLKALLEEAARSAQPVILFVDEVHTLVGAGGTSGTGDAANLLKP
                     ALARGTLRTIGATTWSEYKRHIEKDPALTRRFQVLQVAEPEEVAAVEMVRGLVTTLEE
                     HHDVLILDEAVRAAVQLSHRYIPARQLPDKAISLLDTAAARVALSLHTPPARVQYLRQ
                     QLNAAELEQELLLKQEKMGIRSERGDVLTARIISLNADLSDTEARWQQELELVRSLQE
                     QRGAAPGERDEITLQQAETALRGWQGENPVVYPEVSATVVAEIVSDWTGIPAGRMVKD
                     EASQILELPTRLAQRVTGQMDALAQIGERIQTARAGLGDPRKPVGVFLLAGPSGVGKT
                     ETALALAEAIYGGEQNLITINMSEYQESHTVSTLKGAPPGYVGYGEGGVLTEAVRRHP
                     WSVVLLDEIEKAHHDVHELFFQVFDKGAMEDGEGVHIDFKNTTLLLTTNAGAELISQL
                     CEDPALMPDSTGLKEALMPELRKHFPAAFLGRVTVIPYLPLDETARGVIARLHLGRLM
                     DRMAEQHGVSLVYGDELITHIVSRCPMHETGARLLIGYIEQHILPQLSRYWLQAMTEK
                     TAITQIRIRVDEDEQVIFETVHEA"
     gene            2749..3300
                     /gene="tssB"
                     /locus_tag="J1Z28_003538"
     CDS             2749..3300
                     /gene="tssB"
                     /locus_tag="J1Z28_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBA7718341.1"
                     /translation="MAINNSAQKFIARNRAPRVQIEYDVEIYGSEKKIELPFVMAVLA
                     DLAGKPREELPPVTDRKFLDIDIDIDNFNERMKAIAPRVAFAVPNTLTGEGQLMVDIT
                     LENMDDFSPAQIARKVDALNQLLEARTQLANLQTYMDGKAGAENLINKLLQDPTLLKA
                     LASAPKPVAQQESVLSGETGTAD"
     gene            3325..4839
                     /gene="tssC"
                     /locus_tag="J1Z28_003539"
     CDS             3325..4839
                     /gene="tssC"
                     /locus_tag="J1Z28_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HBA7718342.1"
                     /translation="MVNDTLLATGDLSTTQTTAYQETEFDSLLDKAFRPKTPQAAKAV
                     EAAVQTLAQQALTNTVTVSDDAYKSIAAIIEQIDFKLTEQIKLILSHPEFQKLESSWR
                     GMEHLVYNTETDEKLKIRFMNLSKDELRKNMKRYKGIAWDQSPMFKKLYEAEYGQLGG
                     EPYGCIIADYYFDHTPPDVDLLGSIAKVAASAHAPFIAGASPSVLQMDSWQELANPRD
                     LTKIVTQNLEYAPWNSLRKSEDSRYIGLTMPRFLSRLPYGAKTNPVDEFDFEEDADGA
                     DHSKYVWSNAAYAMGVNINRSFKHYGWCTLIRGVESGGAVENLPCHTFPTDDGGVDMK
                     CPTEIAISDRREAELAKNGFIPLIHRKNSDYAAFIGAQSLQKPQEYYDPDATANANLS
                     ARLPYLFACSRFAHFLKCIVRDKIGSFKEREDMQRWLNEWIMNYVDADPVNSSQETKA
                     RRPLAAAEVVVEEVEGNPGYYDAKFFLRPHFQLEGLTGSLRLVTKLPSVKQGNS"
     gene            4890..5297
                     /locus_tag="J1Z28_003540"
     CDS             4890..5297
                     /locus_tag="J1Z28_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSL0067 family protein"
                     /protein_id="HBA7718343.1"
                     /translation="MAYVYGLVDSLQGTDMVGMAGAGDRKECVFLVQVYAHVGNTGTW
                     KQGRKVFGDKSIPRGTAIATFVNGKYPSRQNDHKHAAFYLEQDSQYIYVMDQWTGKTT
                     KVSTRPIARKGGMRSDGTYPDASNNAEAFYIIE"
     gene            5943..6428
                     /locus_tag="J1Z28_003541"
     CDS             5943..6428
                     /locus_tag="J1Z28_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HBA7718344.1"
                     /translation="MAYDIFLKIDGIDGESMDDKHKNEIEVLSWRWNIHQESTMHAGS
                     GLGSGKVSVTNLEFEHYIDRASPNLFKYCASGKHIPKSILVMRKAGGNPLEYLKYTFT
                     DLIVAVVSPSGSKEGEIASRERVELSFSTVKQEYVVQNQQGGSGGTITAGYDFKANKE
                     I"
     gene            6496..7029
                     /gene="tssJ"
                     /locus_tag="J1Z28_003542"
     CDS             6496..7029
                     /gene="tssJ"
                     /locus_tag="J1Z28_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBA7718345.1"
                     /translation="MNKWRNPGGWLCAVALSFVCLLSGCSIDSDDVPDLMSQRLDLVM
                     KASDKVNPDNQKKAAPIEIRVYELKNDAAFTAADWWSLQDNDKAVLADDLLHRDSFIL
                     RPGEEKKLRRSLNKETTTLGILAGYRNLGKSVWRATYKIPDPPEKSWYSRFMPKKKVE
                     LDAGIEQSAIVIKERDE"
     gene            7033..8376
                     /gene="tssK"
                     /locus_tag="J1Z28_003543"
     CDS             7033..8376
                     /gene="tssK"
                     /locus_tag="J1Z28_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HBA7718346.1"
                     /translation="MSWNNRVVWSEGQFLLPQMFQQQERYLEHVIHYRSLPLSPFFWG
                     FNEYGIGSEALNIGKLVLTKASGVFADGTPFNAPGHTPLPPPLTILPEHLNQLIYLAV
                     PIRTPNSEETSFDNNPESLARFSVHENEIRDANSLGRGAQLLQLSHLRLRLLPEKAVT
                     DAWIGLPLTRITALHPDGRVDIDKELIPPVANYQASTLICTWLSWINDLIKMRADSLA
                     ERLTGSDKHGHEAAEVSDYLLLQVLNRFGPLLIHMSKTPLSPEVLYRNLCALAGELST
                     YVRPQTRRPEEYKEYKHLTPYAGLKSVVDEVQYLLNAVLIRGAQRIPLEEGTYGILNA
                     VVEPTELSDFSSLVLAVKAQMSADTLLQHFSAQTKIGPSERLPELIRSHLPGLVLQVL
                     PVPPRQIPFQSGYIYYDIRRDGVLWEHIARYGGMAMHTAGEFPGLELALWGVRDK"
     gene            8373..9689
                     /gene="tssL"
                     /locus_tag="J1Z28_003544"
     CDS             8373..9689
                     /gene="tssL"
                     /locus_tag="J1Z28_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL"
                     /protein_id="HBA7718347.1"
                     /translation="MTDEILTPPAANTLPFQPSAGGVKPPDNDGYDAPIQDSLRREEV
                     KVVVEDGPDMKLRLAEITAAVNPLLAAASPLLCALAQMPRELSPGLVEHYRALLEREV
                     RRYQTLCDQANLRREHVLAVRYCLCTALDEAANNTVWGRRGVWAGKSLLVTFHGESEG
                     GIKLFQIIGRLAASFQEHSDVLEIIYHILGLGFEGRYSVRPDGRKQLDDIRQQLLTQL
                     VQRRDPVTPTLSPEFAGAVSGRMRRMRRVPVWLSAGIALLVMLTLFAFYSHRMNLATE
                     TVKQRIDAIGKNLPPPPVPVHKLRLKILLANEIARGLLTVEEDDHHSRVIFRGDTMFI
                     PGQKTVNDALRPVIDKAAREIARVGGAVTVTGHTDSQPIYSAEFPSNQVLSEKRAAEV
                     ASLLTSGGVPASRIRGVGKGDTMPVADNSTKTGRAQNRRVEILVVE"
     gene            9697..10383
                     /locus_tag="J1Z28_003545"
     CDS             9697..10383
                     /locus_tag="J1Z28_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718348.1"
                     /translation="MEKLECNASNHSPVWGVTEFIFWKLAPKKWGGGVAFGRYFKDGW
                     VKHNCLLINAAANAWHLPAPLLAGVCWIEVGGDPQIIDTLAFEFRAMSWSGPEWVDEH
                     ITIASKPEKTSFGAVSMQLRTAARTLGLDETKMDNSQLRELANCLQHDVFNIQLAARH
                     LYQLAEKDGFQVSLPHLTKEQIRIIGARYNRGVSLSLEKIKEDTSYGDFIINNWLHFS
                     SLLITTWKSS"
BASE COUNT         2602 a         2465 c         2850 g         2471 t
ORIGIN      
        1 acagatggac actcctgttt cgcgcagtgc actgtatgga aagctaacca aatctttgtt
       61 tagatctttg gaatctgcga cgacgttctg taaattacgc gctaatcctt atgtcgaact
      121 gacgcattgg ttgcatcagt taatacaaca accagagaat gacattcaca atgtgcttcg
      181 acattatcaa ataacatctg ctgatgttga gaaggcgtta attcagcaat tgaatatatt
      241 gcctgccgga gcgagtgtaa ttaacgattt ttcccatcat atcgatcttt ctgttgaaaa
      301 agcctggatg ttggcaagta tccgctttgg tgatcaaaaa attcgtagtg gatggttatt
      361 acttgcctgg ttaaccacgc cagaattgta tcgggtgtta aaaaatatct gtgcgccatt
      421 ggcagggcta ccggtagatg aactattaga tattcttccg tctttaatcg cttcatctcc
      481 tgaattaaca gaaatacctt atgacggctc ggatttaaat tttgcagtac cgggagaagc
      541 tagccaggtc tttgccggcg aatcaaccgg aagtaaatcc gcgctggcga aatactgcca
      601 ggacatgact gcgctggccc gtgaaggcaa tatcgatccg gtcaccggcc gcgaacacga
      661 gatccgtact atgacggata ttctgctgcg ccgtcgccag aacaatccgc tgctgaccgg
      721 cgaggcgggg gtcgggaaaa cggcagtggt ggaaggattc gcgctggcga ttgccggggg
      781 ggaagtgccg ccttcactgc gtaacgtgcg gttgctgtca ctggatgttg gagcgctgct
      841 ggctggggcg tccatgaagg gtgaatttga atcccgcctg aaagctttgc tggaagaggc
      901 cgcgcgctcg gcgcagccgg tgatactttt tgttgatgaa gttcacaccc tggtgggcgc
      961 tggtggtaca tccggtacgg gggatgctgc caacctgctg aaaccggcgc tggcgcgtgg
     1021 cactctgcgc accatcggcg caaccacctg gagcgagtac aaacgccata tcgaaaagga
     1081 tccggcgctg acccggcgtt ttcaggtatt gcaggttgct gagccggaag aggttgcggc
     1141 tgttgagatg gtgcgcggat tagtcaccac gcttgaagaa caccatgatg tcctgattct
     1201 tgatgaggcc gtgcgcgccg ctgtacagct ttctcaccgt tacattccgg cccggcaact
     1261 accggataag gccatcagcc tgctggatac ggcggcggcc cgtgtagcgc tgtcgctgca
     1321 taccccgcct gcgagagtcc agtatctgcg ccagcagctc aacgctgccg aactggagca
     1381 ggagttgtta ttaaagcagg aaaaaatggg tatccgctcg gagcggggcg atgtactgac
     1441 ggcgcggatt atatcgctta atgccgatct ttctgacacc gaagcgcggt ggcagcagga
     1501 actggagttg gttcgcagtt tacaggagca acgcggcgca gcgcctggcg agcgcgacga
     1561 aatcactcta caacaggcag aaacggcatt aagaggctgg cagggtgaaa accccgtcgt
     1621 gtacccggaa gtcagtgcaa ccgtggtggc agagattgtc tccgactgga ccggtattcc
     1681 cgccggacgg atggtgaaag atgaagccag tcagatactg gaattgccca cccgtttggc
     1741 gcagcgtgtt accgggcaga tggatgcgtt ggcgcagatt ggcgagcgta tccagaccgc
     1801 gcgggcgggt ttaggtgacc cgcgtaagcc tgtgggtgtc tttttgctgg ccgggccgtc
     1861 cggagtaggc aagactgaga ccgcgctggc actggcagaa gcaatctatg gcggtgaaca
     1921 gaatctgatc accatcaaca tgagcgagta tcaggagtct cacacagtct ccactctgaa
     1981 aggcgcgccg cccggctacg tcggctatgg cgaaggcggt gtgttgacgg aagcggtacg
     2041 ccgccatccc tggagcgtgg tattgctgga tgaaattgaa aaagcccatc acgacgtcca
     2101 cgagctgttc ttccaggtgt ttgataaggg ggcgatggaa gatggcgaag gtgtccacat
     2161 cgatttcaaa aataccacgc tgttgttgac tactaacgcg ggggcggaac ttatcagcca
     2221 gctctgtgag gatccggcgc tgatgcccga ttcgacaggg ttaaaagagg cgttaatgcc
     2281 ggagttacgt aaacacttcc cggcggcttt cctggggcgt gtgacggtta ttccttattt
     2341 accgctggat gagaccgcgc gtggtgttat tgcccgcctg catcttggca gattgatgga
     2401 tcggatggcg gagcagcatg gcgtttcact ggtgtatggc gatgagctga ttacgcacat
     2461 tgtatcgcgc tgcccgatgc acgaaaccgg cgctcgtttg ctcatcggct atatcgaaca
     2521 acatatcctc ccacaactgt cccgttactg gttgcaggcg atgacagaga aaacggccat
     2581 cacgcaaatc cgcattcgtg tggatgagga tgaacaggtt atttttgaaa cggttcatga
     2641 agcatgattt atccgtatga atgaactgtt tgctgtgcat tttttaaact gtgccgtcgg
     2701 accactcccc aggtggtcgt cggctgactg ataaggaaat gaaggattat ggctatcaac
     2761 aacagtgcgc agaagtttat tgcccgcaac cgcgcgcccc gcgtgcagat tgagtatgac
     2821 gttgagattt acggctccga gaaaaaaatc gaactaccgt ttgtcatggc ggtactggcc
     2881 gatctggccg gtaaaccacg cgaagagctg ccaccggtca ccgatcgtaa attccttgat
     2941 attgatatcg atatcgataa cttcaacgag cggatgaaag ccattgcgcc gcgcgtggca
     3001 tttgctgtcc ccaatacgct gacgggcgaa gggcagttaa tggtcgatat cacgttagag
     3061 aatatggacg atttttcgcc tgcgcagatt gcccgtaagg tggatgccct gaatcagttg
     3121 ctggaagcac gtactcagct tgccaacctg caaacctata tggatggtaa agcgggagca
     3181 gaaaatctga tcaacaaact attacaagac ccaacgttac tgaaagctct ggccagcgcg
     3241 ccaaaacctg tcgctcaaca ggaaagtgtc ttatcaggtg aaacgggaac agcagactaa
     3301 cagcgatttt attaaggaac tttcatggta aacgatactc ttctggcgac tggcgacctc
     3361 tctactacgc aaaccactgc ctatcaggaa acggaatttg attcgctgct ggataaagcc
     3421 ttccgtccca agacacctca ggcagcaaag gctgttgagg cggcagttca gacgctggcc
     3481 caacaggctc tgacaaatac ggtcactgtt agtgatgatg cttataaaag tattgctgcg
     3541 attattgagc agatcgattt caaattaact gagcagatca aactaatcct gagtcatcca
     3601 gaatttcaga aactggagtc ctcttggcgt gggatggaac atttggttta caacactgaa
     3661 accgatgaaa aattaaaaat ccgctttatg aacctttcaa aggatgaatt gcggaaaaat
     3721 atgaaacgtt acaaagggat cgcgtgggat caaagcccca tgttcaaaaa gctgtatgaa
     3781 gccgaatatg ggcagttagg cggagaacct tacggttgta ttattgcgga ttattatttt
     3841 gatcacacgc caccggatgt ggatctgctc ggctccattg ccaaagtggc agcctcagcc
     3901 catgcgccgt ttattgccgg ggcctctcca tcagtgttgc agatggattc ctggcaggaa
     3961 ctggctaacc cacgcgacct caccaaaatt gtgacccaga acctcgaata tgcaccgtgg
     4021 aactcactgc gtaaaagcga ggattcccgc tacatcggcc tgacgatgcc gcgttttctc
     4081 tcccgtttac cttatggtgc gaaaacaaac cctgtcgatg agtttgattt tgaagaagat
     4141 gcagatggcg cagaccacag caaatacgtc tggagcaacg ctgcctatgc gatgggcgtg
     4201 aatatcaatc gttcctttaa acattatggc tggtgtacgt tgatccgcgg tgtggaatcc
     4261 ggcggcgcgg tggaaaacct gccctgtcat accttcccga ccgatgacgg cggcgtggac
     4321 atgaaatgcc cgacggaaat cgccatctct gaccgccgtg aagcggaact ggcgaaaaat
     4381 ggctttatcc cgctgatcca ccgcaaaaac tccgattacg ccgcctttat cggcgcacag
     4441 tcgctgcaaa aacctcagga atattacgac ccggatgcca cggccaacgc taatctctcc
     4501 gccaggctgc catatttgtt cgcctgctca cggtttgcgc acttcctcaa gtgcatcgtg
     4561 cgcgacaaaa tcggctcgtt caaagaacgt gaagatatgc agcgctggct caatgagtgg
     4621 atcatgaact acgttgacgc cgatccggtg aactcctcac aggaaaccaa agctcgtcgc
     4681 ccgctggcag ctgccgaagt ggtggtggaa gaggtggaag gtaatccggg gtattacgat
     4741 gccaaattct tcctgcgtcc gcatttccag cttgaagggt taacaggatc actgcgtctg
     4801 gtcacaaaac tgccgtcagt aaaacagggt aattcctgat ttttatgaat attttaatga
     4861 atgacgccgg gtaatactca gggggatata tggcatatgt ttatggactg gtagattcat
     4921 tgcagggcac tgacatggtg ggaatggcgg gggcaggaga cagaaaggaa tgtgtgttct
     4981 tagttcaggt atatgcacat gtggggaata ccggaacctg gaagcaggga cgaaaggttt
     5041 ttggggataa atcgatcccc agaggaacag caattgccac ttttgttaac ggaaagtatc
     5101 cctcgaggca gaatgatcat aagcatgccg ctttctatct ggaacaggat agtcagtata
     5161 tttatgttat ggaccagtgg acaggtaaaa ccacgaaagt ctctactcgc cctattgccc
     5221 gaaaaggtgg gatgcgcagc gatggaacat atcctgatgc cagtaataat gcggaagctt
     5281 tttatattat tgagtagagg atagattcaa tggggacacg ttttattttt tctctgttat
     5341 tggggtttat gttgttttat tcacaatttg ttgcagcatg ggaagtcagt tgccccatgt
     5401 ttatcaatat aaaatcctca acaacagtac tggagtcaga tatatctgca tcatggcaga
     5461 gatcttcccg ctatgaaccc cgtctgtggt tggacggtgt agggatgtcg cagggaaaac
     5521 ctgaaaatcg ggtggatcta aaaccagaga cagagacaat aaagggtgaa accaggcaaa
     5581 tatgggatac cacaatggcc agtaatcagc atagtgaacg ttactgggtt tcctgtattt
     5641 ataaccatgg acaggtttgg ttatcacaac cggtgcctgc attggtgaca cagtgccagg
     5701 tatcttcttt gccaggtcaa cgggcaccag gagagtcatc ggtacctgta atatgtaaat
     5761 agatataata cattcagttt gtgaattaat ggcatgccgg tatctcatta ccggaaatta
     5821 tatagtcttc tgttcttctg aaattaagga gggggggagc tgtacggcaa tgatttacct
     5881 gtatccagat gtacagataa atcagagatt atttttaagt aattcttaat aaggaaatga
     5941 aaatggctta tgacattttt ctgaaaattg acggcattga cggcgagtca atggatgaca
     6001 agcataaaaa tgaaattgag gtgctgagct ggcgttggaa tatccaccag gagtccacca
     6061 tgcacgcggg tagcggtctg ggatccggta aagtctccgt gactaacctg gagtttgaac
     6121 attacatcga tcgcgccagc ccgaacctgt tcaaatactg cgcttctggt aagcatattc
     6181 cgaagtccat ccttgtcatg cgtaaagcag gcggcaatcc gctggaatac ctcaagtaca
     6241 cctttactga cctgattgtg gctgtggttt ctcctagtgg tagcaaggag ggggagatcg
     6301 cttcccgcga gagagtcgaa ctttctttca gcaccgtgaa acaggaatac gtggtgcaga
     6361 accagcaggg cggcagtggc ggcactatca cggcaggtta cgatttcaaa gctaacaaag
     6421 aaatttaagc aatattttcc cggccaggca tatagcctgg ccggttttta ttatttgatt
     6481 ttgaaggaaa ttacagtgaa taaatggcgt aaccccggtg ggtggttatg tgcggtagct
     6541 ttgtcttttg tctgcctgct ttccgggtgc agcattgaca gcgacgacgt gccggacctg
     6601 atgtcacaaa gactcgatct ggtgatgaag gcttccgata aggtgaatcc tgacaatcag
     6661 aaaaaagccg cgcccataga gatacgcgtt tatgaactga aaaatgatgc agctttcacg
     6721 gcggcggact ggtggtccct tcaggacaat gacaaggctg ttcttgctga tgatttactc
     6781 caccgcgaca gctttatcct gcgtccgggc gaggagaaaa aattgcgccg ctccctcaat
     6841 aaggaaacca ccacgctggg gatccttgcc ggataccgca atctgggaaa atcggtctgg
     6901 cgggctacat ataagattcc tgacccaccg gaaaaaagtt ggtacagccg ctttatgcca
     6961 aagaaaaaag tcgaactgga tgctggcata gagcaaagcg ccatagtgat taaagaacgg
     7021 gatgagtaaa ttatgagttg gaataaccgg gtggtctgga gtgaaggaca atttttgctg
     7081 ccacagatgt ttcagcagca ggaacgctat ctggaacatg tcattcatta tcgcagtttg
     7141 cctctttcac cttttttctg gggcttcaat gagtatggta ttggtagtga ggcgctgaac
     7201 atcggtaaac tggtacttac gaaggcttcc ggggtatttg ctgacggtac accttttaat
     7261 gcgccggggc acacaccact cccgccgcct ctgaccatcc ttcctgagca tcttaaccag
     7321 ttgatttatc tggcagtgcc aatacgtacg ccgaacagtg aagaaactag cttcgacaat
     7381 aaccctgaat cactggcacg cttttcggtg catgaaaatg aaatccgtga tgccaattcg
     7441 ctggggcgcg gcgcgcaact gctgcaactt agccacctac gcttgcggct attaccggaa
     7501 aaggcggtga cagacgcgtg gatcggcctg ccgctgacgc gcatcacagc gctgcatccc
     7561 gatggtcggg tcgatataga caaagaattg atccctccag tggcgaacta ccaggccagt
     7621 acgctgatat gtacctggct atcgtggatt aacgatctga taaaaatgcg tgcagattca
     7681 ctggcggaac gattgacggg gagcgacaaa cacggtcatg aagcggcgga agtgtcggat
     7741 tatctgcttc tacaagttct taatcgtttc ggccccttac tgatccatat gtcgaaaacg
     7801 ccgctgtcgc ctgaagtact ctatcgtaac ctctgcgcat tggctggaga actctctacc
     7861 tacgtgcgtc cgcaaacacg ccgtccggaa gaatataaag aatataagca tctgacgccc
     7921 tatgccgggc tgaaatcggt ggtggacgag gttcagtatc tgctgaatgc tgtgttaata
     7981 cgtggggcgc agcgaattcc tctggaagag ggaacttacg gtattctcaa tgcggtggtt
     8041 gaacctaccg aactgtcaga cttcagctcg ttagtattgg ctgtgaaggc acagatgtcc
     8101 gccgacacct tactacaaca tttttcggct cagaccaaaa tagggccgtc agaacgcctg
     8161 ccggaactga ttcgctcgca ccttcctggt cttgttctac aggtgctgcc agtgccaccg
     8221 cgtcagatcc cgttccagtc cggttacatc tattacgaca tccgccgcga cggcgtgttg
     8281 tgggaacata tcgcccgcta tggtggcatg gcaatgcata ccgccggaga gtttccaggg
     8341 ctggagctgg cgctgtgggg agtgcgcgat aaatgaccga cgagatcctg acgccgcctg
     8401 cggcaaacac cctgcccttc cagccatcag ccgggggagt aaaaccgcca gacaatgacg
     8461 gttacgacgc gcccatacag gacagtcttc gcagggaaga ggtaaaagtg gtggttgaag
     8521 acggcccgga tatgaagctc aggctggccg aaatcaccgc tgccgtgaat cccctgctgg
     8581 cggcggccag ccccctgtta tgcgcgctgg cgcaaatgcc tcgtgaactt tcccctggtc
     8641 ttgtcgaaca ttatcgcgcc ctactggaac gtgaagttcg ccggtatcag acattatgcg
     8701 accaggcaaa cctgcgccgc gaacatgtgc tggcggtgcg ctactgcctg tgtacggcgc
     8761 tggacgaagc cgccaacaac accgtctggg gacgacgcgg tgtctgggcc gggaaaagcc
     8821 tgctggtgac ttttcatggc gaaagtgaag gcggcatcaa gctgtttcag ataattggtc
     8881 gtctggccgc cagcttccag gagcacagcg atgtgctgga aatcatttac cacattctgg
     8941 ggctgggttt tgaaggtcgc tacagtgtac gacccgacgg gcgcaagcaa ctggacgata
     9001 tccgccagca actgctgact caactggtcc agcgccgcga tccggtcacg cccacgctct
     9061 cgccggagtt tgccggtgcc gtcagcggac ggatgcgtcg gatgcgtcgg gtaccggtct
     9121 ggctgagcgc cgggattgcc ctgctggtga tgctgacgct gtttgccttt tacagtcacc
     9181 ggatgaacct cgccaccgaa accgtaaaac agcgtatcga cgccattggt aaaaatctac
     9241 cgccgccacc cgttccagtg cataaactgc ggttgaaaat cctgctggcg aacgaaatcg
     9301 cccgtggcct gctgacggtg gaggaagatg accatcacag ccgtgtaatc tttcgtggcg
     9361 atacgatgtt tataccgggg cagaaaaccg tgaatgatgc gctgcgcccg gtgattgaca
     9421 aggccgcgcg tgagattgcg cgtgtggggg gcgcggtgac ggtgaccggc cacacggaca
     9481 gccagccgat ctactccgct gaattcccgt ccaaccaggt attgtcggaa aaacgtgcgg
     9541 cagaagtagc ttcgctgctg acatctggtg gcgttccggc tagccgaatt cggggtgtcg
     9601 gtaaagggga tactatgccg gtggctgata atagtactaa aactggacga gcgcagaatc
     9661 gccgggtgga aattctggtg gtggagtgaa ttaataatgg aaaagctaga gtgtaatgcg
     9721 tctaaccatt cgccagtgtg gggagtaaca gaatttatat tctggaaact ggcgcctaaa
     9781 aaatgggggg gaggcgtagc atttgggcgt tattttaaag atggttgggt gaagcataac
     9841 tgtcttttga tcaatgcagc tgcaaatgcc tggcatttac ctgccccctt acttgctgga
     9901 gtttgttgga ttgaagtagg gggtgatcct cagattatag acacgctggc atttgaattt
     9961 cgtgctatga gttggagtgg gcctgaatgg gttgatgaac atatcacaat cgcttcgaaa
    10021 ccagaaaaaa cttcttttgg tgcggtgagt atgcaattgc gaactgcagc cagaacactc
    10081 gggcttgatg agactaaaat ggacaactct cagttgcgtg agctggcaaa ttgtctccag
    10141 catgatgtgt ttaatataca attagctgca agacatcttt atcaattggc tgaaaaagat
    10201 ggttttcagg tatccttacc tcacctgaca aaagaacaaa taagaattat tggagctcgt
    10261 tataatcgag gtgtttccct tagtctggaa aaaataaagg aagataccag ctatggcgat
    10321 tttattataa ataactggtt acatttctct agtttattaa taactacctg gaagtcttca
    10381 tgagaaaa
//
LOCUS       DADSZD010000151        10324 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.152, whole genome shotgun sequence.
ACCESSION   DADSZD010000151 DADSZD010000000
VERSION     DADSZD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.152"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..588
                     /locus_tag="J1Z28_003546"
     CDS             <1..588
                     /locus_tag="J1Z28_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718349.1"
                     /translation="LRYLAKNSRFRRGIVAQFLYVGMQVAVWSFTIRLALELGDINER
                     DASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYVALAPSFSAVYV
                     AVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPAIQGYIADMFHS
                     LQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            600..1613
                     /locus_tag="J1Z28_003547"
     CDS             600..1613
                     /locus_tag="J1Z28_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 domain-containing protein"
                     /protein_id="HBA7718350.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLTGRYEYIMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            2035..2289
                     /locus_tag="J1Z28_003548"
     CDS             2035..2289
                     /locus_tag="J1Z28_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBA7718351.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVTLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPLTNSSGIGVELLPVEMTLG"
     gene            complement(2536..3792)
                     /locus_tag="J1Z28_003549"
     CDS             complement(2536..3792)
                     /locus_tag="J1Z28_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA7718352.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGASLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(3805..4092)
                     /locus_tag="J1Z28_003550"
     CDS             complement(3805..4092)
                     /locus_tag="J1Z28_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBA7718353.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSISADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            complement(4108..4551)
                     /locus_tag="J1Z28_003551"
     CDS             complement(4108..4551)
                     /locus_tag="J1Z28_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7718354.1"
                     /translation="MLKNLLNTKVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            4822..5853
                     /locus_tag="J1Z28_003552"
     CDS             4822..5853
                     /locus_tag="J1Z28_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7718355.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMENRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDSCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            5981..6541
                     /locus_tag="J1Z28_003553"
                     /pseudo
     CDS             5981..6541
                     /locus_tag="J1Z28_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(6560..7043)
                     /locus_tag="J1Z28_003554"
                     /pseudo
     CDS             complement(6560..7043)
                     /locus_tag="J1Z28_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(7829..7993)
                     /locus_tag="J1Z28_003555"
     CDS             complement(7829..7993)
                     /locus_tag="J1Z28_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate delta-isomerase"
                     /protein_id="HBA7718356.1"
                     /translation="MRGTIDALDELFATLSYDKPRVIALHFKISEGYTLLMLITTFAS
                     CLILCKNYGW"
     gene            complement(8105..8794)
                     /locus_tag="J1Z28_003556"
     CDS             complement(8105..8794)
                     /locus_tag="J1Z28_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003825705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBA7718357.1"
                     /translation="MRTVLDSLKGKLIVSCQALENEPLHSPFIMSRMALAAAQGGAAG
                     IRANSVADISAIKEIVSLPMIGIIKRDYPGSEVFITATLREVDELMTVEPEIIALDAT
                     ARPRPGGQTLEELVTQIRARYPSVLLMADIATVEEAVTAERLGFDCVGTTLYGYTAET
                     ARHVLAENDCGFLREVLAAVSVPVVAEGNVDTPERAARCLTLGAHTVVVGGAITRPQQ
                     ITTRFIAAIAS"
     gene            complement(8794..>10324)
                     /locus_tag="J1Z28_003557"
     CDS             complement(8794..>10324)
                     /locus_tag="J1Z28_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA7718358.1"
                     /translation="SGAWFEKAQRFGKSFMLPIAVLPAAGLLLGIGGALSNPNTLTAY
                     PFLNVDWLQAIFTIMTSAGSIVFANLSVLFAVGVAVGLAKSDKGTAGLAALLAFLVMN
                     ATINALLTLNGTLAHKNPGSVGQGMTLGIQTLETGVFGGVVIGLVTCTLHSRFNKVSL
                     PQFLGFFSGSRFVPIISSLAAIVVGAVMTVVWPHFQKLIFGLGGLVDATGYLGTLLYG
                     FILRMLGPLGLHHIFYLPFWTTALGGSEIVNGQLVEGTQRIFFAQLADPNTQQFYAGT
                     ARFMSGRFITMMFGLLGACLAMYHTAKPENRKVVAGLLLSAALTSFLTGITEPVEFSF
                     LFVAPVLYVIHAFFDGLAFMVAHILHITIGQTFSGGLIDFLLFGVLQGESKTNWMYVP
                     IVGIPWFFLYYFTFRYLINRFGWLTPGRENVTLVESGQPQSERAAAVIAGLGGKENLE
                     EVDCCATRLRVTVKESSKVDEAALKVTGARGVIIRGNGVQVIYGPHVTIIKNEVEEIL
                     S"
BASE COUNT         2792 a         2590 c         2370 g         2572 t
ORIGIN      
        1 ctgcgctatc tggctaaaaa ttcgcgcttt cgtcgtggta ttgtggctca attcctttat
       61 gtgggtatgc aggtcgcagt atggtcattt accatccgtc tggcgctgga gttgggcgat
      121 atcaacgaac gtgatgcttc aaacttcatg gtctacagct ttgcctgctt ctttattggc
      181 aagtttatag ccaacatcct tatgactcgt tttaatccgg aaaaagtcct gattctctat
      241 tccgttatag gggcgctgtt tctggcatat gttgcactgg ctccaagttt cagcgcggtc
      301 tatgtcgccg tcctggtaag tgttttgttt ggcccttgct gggcgacgat ctatgcagga
      361 acactggata cagttgataa cgaacataca gaaatggcag gagcagtcat tgttatggct
      421 attgtcggtg ccgcggtggt tccggcaatt cagggatata ttgcagatat gttccactca
      481 ctacagcttt cattcctggt ttctatgctc tgttttgtct atgtcggagt ctacttctgg
      541 cgtgaaagca aagtacgtac cgcactggct gaagtcacag cgtcctgagg agaaccgtta
      601 tgagtacgcg tattaatttg tggcgggcgc tttttggcga gaaacctcgt atcttgctgg
      661 aaaatagtga ttttactgtc acgtcttttc gctatgacag cggtgtcgag gggcttaaaa
      721 ttgcaaacag tcgtggacat ttgattattc ttccctggat ggggcagatg atttgggatg
      781 cgcaatttga tggacacggc ctcaccatgt gcaatatgtt tcgccagcct aaaccggcga
      841 cagaggtgat agagacctat ggctgttttg cttttcattc aggtttgctg gctaatggct
      901 gcccatctgc ggaagatact catctgctac acggtgaaat ggcctgtgcc gcgatggatg
      961 aagcatggct ggaattagac ggagatatgt tacgccttac cggacgttac gagtatataa
     1021 tgggtttcgg tcatcattat ctggctcaac caacagtggt ccttcacaaa tcctctacac
     1081 tgttcgatat taagatggcc gtaaccaatc tggcttctgt agatatgcca ctacaatata
     1141 tgtgccacat gaattatgca tatataccaa atgctacatt cagccagaat atcccggacg
     1201 aaatattaag attgcgtgaa tcagtgcctt cgcatgttaa tcccaccgct cagtggctgg
     1261 cgtttaatca acggattatg cagggagaag cctctctgtc gacgctgagt caaccggaat
     1321 tttatgatcc agaaattgtc ttctttgcag acaaactgga cgcttataca gatcagccag
     1381 agtttcgcat gatctctccc gatggcacta cgtttgttac tcgtttctac agtgctgaac
     1441 taaattatgt gactcgctgg atcctttata acggagagca acaggtagcc gcctttgcat
     1501 tacctgcaac ctgtcggcct gaggggtatc ttgccgcgca acgtaatggc acgctgattc
     1561 aggttgcacc gcagcaaacg cgcactttta cagtcaccac aggaattgaa taaccactca
     1621 agtcctgaac agcggcgcgc ttatcgttaa ggtaagcgcg cttttttacc cgccaggaca
     1681 agactatgat agcgttgccc ttatagacga tcacattatc gtccactcca aatttgcgca
     1741 actactgtag agtggtgtac tccacagctt ctttgaggat ctcaacttcc attgttttct
     1801 taccgagcaa gcactgcaac tcttttattt atttgtagtg gtcaacaaaa actggtcaca
     1861 atttcagttt ttttccagta tctattttcc gattcgttcg gcggtaatcc accgttatat
     1921 tcatgaggcc ggagtgcact ataaaagtgt atgcgtaaac cgactgctgt atgtagccat
     1981 taagccagta ttggtaacat aggtacccac cttttcaact agtggacacc tgttatggaa
     2041 caaaaaacat tatcagctga accccgcagg tcattttcaa atgagtttaa acttcaaatg
     2101 gttacactgg ctttacaacc aaggggttct gttgcccgta ttgcccggga gcatgatatc
     2161 aatgataatc ttctgttcaa atggctcagg ctctcgctga tcaaagggcg tatatcacgg
     2221 cgtcttccgt taacgaactc ttctggcatt ggcgttgaat tattacctgt ggagatgacg
     2281 ctgggatgag cggccttacc ccgtttttta cgcgaggtaa ttatgaagat caatgactag
     2341 ccgtcgaacc gtgtgcatga actgctgcac tgaaacttct cagctgtaaa ataatcctta
     2401 cgctacgtac ttctcggagc gcttacaatt aatcggatgc agtcctgact ggaagaaaga
     2461 aaaatggaga aaacaagagg aattaatatc ctccacgttc ctgaaatcct ggaggatatt
     2521 aatgtcatca aaatattagc gaacgataat ccctaacaaa atacccagtg cgccaaagtc
     2581 agcatcactg aaggtggtgt tagcaaaacc aatatcaccc agcaccggca ataagaatac
     2641 tggcaggaag gtaataagca agccttgagc gaaggcaccc aaaatcgcac cacgacgtcc
     2701 acccgttgca ttaccgaata cccctgcagc tgcacctacg aagaagtgcg gcaccacacc
     2761 aggaataatc acgatcatgt tcagcagata caacgtgaac ataccgatta aacctgcagc
     2821 aaagctactc aggaaaccaa ccagtacagc gttaggtgcg taagggaaga cgaccgggca
     2881 atcgagagca ggtctggcat tcggaaccag cttgtctgaa atacctttga atgccgggac
     2941 aatctccgca atcaccatgc gcacaccctg cagaatgatg tacacgccag ctgcgaaggt
     3001 aatggattgc atgatagaga acatgaacca gtttttacca ccgctaagct ctttcaccgc
     3061 atctgcaccc gcaaacaggc acgtcaccag gaaaattatg ctcatggtaa aagagatagc
     3121 aaccggcgtg tcgcgcaaga acagcagact tttcggcaca ttcatgtctt cagtcgagtg
     3181 ctctttatta ccaaacttgc tgccgatgaa accggccaat acataagata atgttgagaa
     3241 gtggccgatg gcaacgtcat cagaaccggt cactttcttc atgtacggat gcgcaatagc
     3301 cgggaaaaag accattgcca cgccaacaac caaagagcca acggctataa gcgtaatgcc
     3361 ggtcatgcct gctgttgcca gaattaccgc taccatcatc gacataaaca acgtgtgatg
     3421 accggtcagg aagataaatt tccacggtgt gaaacgggca atcataatgt tgataaccat
     3481 cgcaaagaac ataatcatcg ccatttcttt cccgaagctt ttctgcgcta cggaaacaat
     3541 ggcttcatta ttcggcacga caccctgaat accaaaggca tgctggaaaa tgtttgcgaa
     3601 gtcacccagt gacgacacaa ctaaactcgc ccctgcgcct aaaatcacaa accccataat
     3661 ggtcttgacg gtacctttaa tgcattcggt taccggtttt ttctgtgcaa tcaggccaat
     3721 cagggcgatt aaaccgacca gcaccgcggg ttcggaaagt acatcgctca tcaaaaaacg
     3781 aaagaaatcc atatcgcccc cagcttacag tgcgcctaat tcggttaatg ccacggacaa
     3841 acgttctctc atggctttct tatcaatcat attttccagg gcgacgattt tgcctgcgac
     3901 agactgcgcg actaactgct cggcaatatc tttggtgccg acatagatat cactcggcgt
     3961 ccctttcgcg gagcccaggt cgacatgatc aacatcagcg ctgatggaca aatccttcaa
     4021 aatgctttta atgctcattt ccatcatcag gctggtgcct aagccgttac cgcaaaccac
     4081 tgttattttc ataaatgttc ctcaggatta atagcgcgca ataacagaca aaatgtcctg
     4141 cgtcgttttg gccgtcaaaa tagcctgtat gtctttttcg ttatcgaata acttggcgag
     4201 ttgagaaatc gcttcaatgt ggctggtact gtcggttgca gccagcacaa tcagtaattt
     4261 cactggatcg ttttcatcgg catcaaaatt cacacctgaa gggatcaggg tcagtgctaa
     4321 tgacagcttg ttagccccct cttccgggcg cgcatgaggc atcgcaatac cagggccaac
     4381 cacatagtac ggaccgatag tgtcgtgaga gtgatagatc gcatcaacat aacgcggttc
     4441 gatagaaccg ttttcaatca acggtcggca tgaaatcgcc acggcttccc gccagtcttt
     4501 gacctgttcc acgacctgaa cgacctttgt atttaataga ttttttaaca tgcgactgat
     4561 aactcccgat gataattaac aggagagtag atattttacg cattaattaa tgtgatgatt
     4621 gtcgcatttg atagcgctat ctgataacgc taccaatctt tgtgatagcg ctatcatttc
     4681 tctgtcgcca tgctttggct atattcggtt cctgcatata caatgcaaca ggatatttgc
     4741 taccgtgggc aaaaactgga aagagtaatg gtaaaataaa agtaatgaaa acagctggtt
     4801 gtaattatcc atggagtcga aatggaaaaa acacgtaagc gccgtggtac cggacgggtg
     4861 acgctgcagg aggtcgctaa ctttgcaggt gtcggaacca tgaccgtttc ccgcgcgctg
     4921 agaacaccgg aacaggtttc tgacaagctg agggaaaaga tagaacaggc cgtagaagag
     4981 ttgggttata ttcccaacag aactgctggc gcccttgcat ctggtcacag tgatacagtc
     5041 gccgtactgg ttccttccct gacagataaa gccagctccc gctttatgca gtcgttgcag
     5101 caggtgctta ataaaaatga attccagcta ctgcttgggt gtcatgagta taatcagctc
     5161 aaagaagctg aaattctgat gacgcttctt caaggcaacc cggcggcgtt agttattttt
     5221 ggttctcagt tggctgataa aactcaccag atactcgaga aaacaaatat acctaccatt
     5281 aacgttgtcg gttccccttt cagtccggct caaattacca tcgaaacagc attttttgaa
     5341 gcatcacata aactgaccga acatctgctg gagcagggtt ataagaatat cggatttatc
     5401 ggtgcgcata tggaaaaccg cctgcaacgt cagcaactga acggctggca taaagcaatg
     5461 ctgagccatt accagaacac tgatcttgtt atcacaatgc cggatgccgc aagtctgcaa
     5521 ttggggcgtt acgcattaaa cgagatatta ctgcgacaac cagagttgga tgctgtgatt
     5581 tgcagccacg aagagattgc gctgggtatc atgtttgagt gtcagcggcg tttgttaaaa
     5641 atccctggaa atatcgctgt agcttgtctt gacggttcag acagttgcga tcaaacacac
     5701 ccaaccttga catccatccg tattgactac aaaaaaatgg ggatagagac gggtaagtta
     5761 ctgattggac tattgaataa taatcatgat gaatcagaag agtcgcgtat cgtgcaattc
     5821 aactaccaga tagaattacg tcagagtaca tgagcctaaa agggcactat tgcaaagtta
     5881 gctattgtgg tagggatagc cgtttccggc tattccccgc gcagatccgg atgtgtgctg
     5941 ctaacgcatc cattcctact tcaggtattt ggcgtagaag cggatgctaa aaacttgccg
     6001 caacaccgca gtatcagccc gcagagtcgc tctccatctc cagattatcc attcctatat
     6061 tatctctgca ccggatgaat tacgcgaaca actcagaaat atgacacata tgcaacttat
     6121 caggactccg ggatcctgga agcctagtgc cagtgaatat cgtaatgtta ccaacgttta
     6181 tcgcattgca ttgaaatcac ttgcctgacg ctatttcgag ttacatgatg aaatcgccga
     6241 ccttgatgtc atgattgcgg caattgtcga tgaactggca cctgaactga ttaaacgtaa
     6301 tgctgttgga tacgaaagcg cttcgcagtt gctcatcact gccggagata atccccaacg
     6361 gctaagaata gaatcaggtt ttgcggtgct gtgtggtgtc aactcggtta ccgtatcttc
     6421 taaaaaaatg aaccgttacc gacttaatcg gggtggggag cgtgctgcaa atagtgcact
     6481 tcacatcatc gctatcggac gtttacgaac tgacgataaa acaaaggaat atgtcgctaa
     6541 atagctgcgc ctaataccgc tacacttttg ccagaccatg tctcccgccg gggaacggac
     6601 tggcagtttc cataaagtgt tggacttttt tcagcaactg ccacatctcc ggcactgatg
     6661 attgcgcatt atcgtttcat gaagcgcctg ccatagccgt tcaaaatgat ttacccatgg
     6721 cgagtaaact tgctgataaa tcaccctgaa cttcggattt tccttcagcc aacgctgcgt
     6781 ttcccggctt ttgtgaataa tgtaattatc cacgatgagg ggcatcgttt ttgcccgccg
     6841 gtacacagac ttcagttgct tcaacagact gataaataag gctgaacttt gcggttactg
     6901 cccacgtagc agactttgcc cgttcagcta tgtaacgcgc cagcaagata atatttttca
     6961 ttctgccccg gtgtcatcac ccgcttttgc tgtccgcgca attgccagtc agcaccggtt
     7021 ttgggatgga tagccaggtt aacagcgtcc cgataacgtc ttgattctta taagggcgtt
     7081 aactaatttc gttacatcat atacatgatg cttgtgtgcg aagtaacgat agacaccaag
     7141 tagatgctta aaggaagagg ctgacataaa actcaaggat atgaactttg cctgcatata
     7201 ggctacacca tggacgtaat agcgttaaaa aagctactct tactaaacag attatacatc
     7261 agatcgcctc gcagattacc tctatattcc cagaagctcc gcccactgcc tgactcagga
     7321 gtttctcgat aacgactatc cgctctatat gccggataca cgcttgtatc acgtgggcct
     7381 ggcgctgcaa aaaatggcgg ctaactatat tgaaagcatc gatcaggacc gcgatttgcg
     7441 gctattgatc tcatttctga gcaaacggtt gggaaaacca gctacgcggc gattctggac
     7501 gacaggcagg tggtttattt ggcaaaagcg ttctcctatg atgaactctc tcattaatgc
     7561 ggcatatcgg atcggcaata atctcccagc cactgtaccg ccgcttgtaa agtgctgctg
     7621 agccaatatc actttctgat aggcttctga agcgtcatgg gcgttcttac gcaataaccc
     7681 gttataccac tgtgaccaca accagacggt accgccgaag caattaaaca accaataatg
     7741 gtgttgataa gacacggaag gatgtactgc tcgccattca gccataacag ttgcagggta
     7801 tatacagcag taatggtaaa accaaccttc accagccgta gtttttgcac aatatcaggc
     7861 agctggcgaa ggtggtaatc agcatcaaca aggtataacc ttcggaaatt ttaaagtgca
     7921 gcgcaatcac ccgcggtttg tcgtagctca gcgtggcgaa tagttcgtcc agcgcgtcaa
     7981 tagtccccct caccttgcag ggtctggaaa atctagttaa tcgggtactg cataacactc
     8041 tcaatatggg gataagcgat aattatcgct tatccccacc aaagtatcat accccatcgg
     8101 tgctttacga ggcgatcgcc gcgataaagc gtgtcgttat ctgctgcgga cgagtgattg
     8161 cccccccaac caccacggta tgagcgccta atgtcaggca acgtgcggca cgttccgggg
     8221 tatcgacgtt cccttccgcc acaaccggaa cgctgaccgc tgccaggacc tcacgtaaga
     8281 atccacagtc attttccgcc agcacatgcc tggcagtttc tgcggtatag ccgtagagcg
     8341 tagtgcccac acagtcaaaa cccagacgtt cagctgtcac cgcctcttct actgttgcta
     8401 tatctgccat cagaagtacg gacgggtagc gagcgcggat ctgcgtgacc agttcttcca
     8461 gcgtctgacc accagggcgc gggcgcgcag tagcatccag ggcaataatt tccggctcaa
     8521 cggtcatcaa ctcatccact tcccggagag tggccgtgat aaacacctca ctgccagggt
     8581 aatcgcgttt gataatccct atcatcggaa gggagacaat ctctttgata gcactaatat
     8641 ccgccacgct gttggcacgg atacctgcag caccaccttg tgctgccgcc agcgccatac
     8701 gtgacataat aaacggacta tgtagaggct cattttccag agcctgacag gagacgatta
     8761 atttaccttt cagggaatcc agtacagttc tcattacgat aaaatctctt cgacttcgtt
     8821 tttaatgatg gtgacatgag ggccataaat cacctgaacg ccgttgccgc gaataatcac
     8881 gccacgcgct ccggtcactt tcagcgcagc ttcatctacc ttactgctct ctttaaccgt
     8941 cacgcgcagg cgcgtggcgc aacagtccac ctcttccaga ttctctttgc ctcccagccc
     9001 ggcaatgact gccgctgcgc gttcactctg aggctggccg gattcaacca gggttacgtt
     9061 ctcacgaccc ggcgtcagcc agccaaagcg gttaatcaaa tagcggaaag tgaagtagta
     9121 aaggaagaac cacggtattc ccactatggg gacatacatc cagtttgttt tagattcccc
     9181 ctgcaacacg ccgaagagaa ggaaatcgat aagcccgccc gaaaaggttt gcccaatagt
     9241 gatatgtaaa atatgggcga ccataaacgc cagaccatcg aagaaggcgt gaattacgta
     9301 taatacgggc gcgacaaaca ggaaggagaa ctcaacaggc tcagtgattc ccgtcaggaa
     9361 agaggtcaag gctgccgaca aaagtagtcc ggcgacaacc tttctattct ccggctttgc
     9421 ggtatggtac attgccagac atgcgccaag caggccaaac atcatggtga taaagcgccc
     9481 ggacataaag cgagcggtac ccgcgtagaa ctgctgcgta ttcggatcgg caagttgggc
     9541 aaagaaaatc cgctgcgtac cttcaaccaa ctggccgttg acgatttcac tgccgcccag
     9601 cgccgtcgtc cagaacggca aataaaagat atggtgtaaa cccagtggac caagcatacg
     9661 caggatgaag ccgtacaaca gcgtccccag atagcccgtc gcatccacca ggccgcccag
     9721 tccaaaaatg agtttctgga aatgcggcca caccaccgtc ataaccgcac ccactactat
     9781 cgcggcaagg gagctgataa tgggtacaaa acgagaacca ctaaaaaaac cgagaaactg
     9841 aggtagtgag actttattga aacggctatg taacgtgcag gtgacgagac caatcaccac
     9901 cccaccaaac acaccagttt ccagtgtctg aataccaagc gtcatcccct ggccaactga
     9961 accggggttc ttgtgcgcca acgttccatt tagcgttaat aaggcgttga tggtagcatt
    10021 catcaccaga aacgctagca atgccgcaag cccggcagtg cctttatccg atttcgccag
    10081 ccccacggcg acgccgaccg caaagagtac cgacaggttg gcaaacacaa tagaacctgc
    10141 gctggtcatg attgtgaaga ttgcctgcag ccagtcgaca ttcaagaatg ggtaggccgt
    10201 cagcgtattg ggattcgaca gagccccacc aatccccaac aacaaccccg ctgcgggcaa
    10261 taccgcaatc ggcagcatga acgatttgcc gaaccgctgc gctttttcaa accatgcgcc
    10321 tgac
//
LOCUS       DADSZD010000152        10306 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.153, whole genome shotgun sequence.
ACCESSION   DADSZD010000152 DADSZD010000000
VERSION     DADSZD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.153"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            124..339
                     /gene="bdm"
                     /locus_tag="J1Z28_003558"
     CDS             124..339
                     /gene="bdm"
                     /locus_tag="J1Z28_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBA7718359.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            441..578
                     /gene="sra"
                     /locus_tag="J1Z28_003559"
     CDS             441..578
                     /gene="sra"
                     /locus_tag="J1Z28_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBA7718360.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            735..2432
                     /locus_tag="J1Z28_003560"
     CDS             735..2432
                     /locus_tag="J1Z28_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HBA7718361.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWHAEYRDYRRTSI"
     gene            2566..3576
                     /gene="adhP"
                     /locus_tag="J1Z28_003561"
     CDS             2566..3576
                     /gene="adhP"
                     /locus_tag="J1Z28_003561"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBA7718362.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3762..4031
                     /locus_tag="J1Z28_003562"
     CDS             3762..4031
                     /locus_tag="J1Z28_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Killer protein"
                     /protein_id="HBA7718363.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTRLRHRLAVIDAACCLADID
                     MPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            4031..4315
                     /locus_tag="J1Z28_003563"
     CDS             4031..4315
                     /locus_tag="J1Z28_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBA7718364.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4544..5197)
                     /locus_tag="J1Z28_003564"
     CDS             complement(4544..5197)
                     /locus_tag="J1Z28_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA7718365.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(5190..6074)
                     /gene="fdxH"
                     /locus_tag="J1Z28_003565"
     CDS             complement(5190..6074)
                     /gene="fdxH"
                     /locus_tag="J1Z28_003565"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HBA7718366.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(6087..9134)
                     /gene="fdnG"
                     /locus_tag="J1Z28_003566"
     CDS             complement(6087..9134)
                     /gene="fdnG"
                     /locus_tag="J1Z28_003566"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8547..8549),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBA7718367.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            9366..10247
                     /gene="yddG"
                     /locus_tag="J1Z28_003567"
     CDS             9366..10247
                     /gene="yddG"
                     /locus_tag="J1Z28_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBA7718368.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2484 a         2702 c         2606 g         2514 t
ORIGIN      
        1 ataaaaatca tcaaccagga ctaatcttaa caacgaagcg gcaaatattt gccatgctga
       61 atgtgcttta taaatctgcg atccgtagca gacaccataa atacacagac acggagaatc
      121 actatgttta cttattatca ggcagaaaat tcaacagcag aacccgctct ggttaatgcc
      181 attgagcaag gccttcgggc agagcacggt gttgtcactg aagatgacat tctcatggag
      241 ctgaccaaat gggtggaagc ttctgataac gacatcctca gtgatatcta ccagcaaacg
      301 atcaattatg tggtcagtgg tcagcaccct acgctttaag gtgctatgct tgatcggcaa
      361 cctaatttag gggtttagca cgtgtttctt cgctacggcg atgttgtcct taaaactagc
      421 aacaggattg aggagttaaa atgaaatcga accgtcaggc acgtcatatt cttggactgg
      481 accataaaat ttctaaccag cgcaaaatag ttaccgaagg tgacaaatcc agcgtggtaa
      541 ataatcccac cggcagaaaa cgccccgctg aaaagtaatt cataaccatc agtcctcaat
      601 gacgattaaa caccattgcc tgcgcaatgg tgtttttgtt tttatctgct ttatacttgg
      661 tacttgacgc cctggcggta atgcaaagac gataaaagcc cccagggatg gatattcaaa
      721 aaagagtgag tgacatggaa ccaaaaacaa aaaaacagcg ttcgctttat atcccttacg
      781 ctggccctgt attgctggaa tttccgttgt tgaataaagg cagcgccttc agcatggaag
      841 aacgccgtaa cttcaacctg ctggggttac tgccggaagt ggtcgaaacc atcgaagaac
      901 aagcggaacg agcatggatc cagtatcagg gattcaaaac cgaaatcgac aaacacatct
      961 acctgcgtaa catccaggac actaacgaaa ccctcttcta ccgtctggta aacaatcatc
     1021 ttgatgagat gatgccagtc atttacaccc caaccgtcgg cgcagcctgt gagcgttttt
     1081 ctgagatcta ccgccgttca cgcggtgtgt ttatctctta ccagaaccgc cacaatatgg
     1141 acgatattct gcaaaacgta ccaaaccata atatcaaagt gattgtggtg actgacggcg
     1201 aacgtattct ggggcttggt gaccagggca tcggcgggat gggcattccg atcggtaaac
     1261 tgtcgctcta taccgcctgt ggcggcatca gtccggccta tacccttccg gtggtgctgg
     1321 atgtcggcac caacaaccaa cagctgctta acgatccgct gtatatgggc tggcgtaatc
     1381 cgcgtatcac tgacgacgaa tactatgaat tcgttgatga atttatccag gctgtgaaac
     1441 aacgctggcc ggacgtactg ttgcagtttg aagacttcgc acaaaaaaat gcgatgccgt
     1501 tacttaaccg ctatcgcaat gaaatttgtt cttttaacga tgacattcag ggcaccgcgg
     1561 cggtaacagt cggcacactg atcgcagcca gccgcgcggc aggcggtcag ttaagcgaga
     1621 aaaaaatcgt cttccttggc gcaggttcag cgggatgcgg cattgccgaa atgatcatcg
     1681 cccagaccca gcgcgaagga ttaagcgagg aagcggcgcg gcagaaagtc tttatggtcg
     1741 atcgctttgg cctgctgacc gacaagatgc cgaacctgct gcctttccag accaaactgg
     1801 tgcagaagcg cgaaaatctc agtgactggg ataccgacag cgatgtgctg tcactgctgg
     1861 atgtggtgcg taatgtaaaa ccagatattc tgattggcgt ctcaggacag accgggctgt
     1921 ttacggaaga gatcatccgt gagatgcata aacactgtcc gcgtccgatc gtgatgccgc
     1981 tgtctaaccc gacttcacgc gtggaagcca caccgcagga cattatcgcc tggactgaag
     2041 gtaacgcgct ggtcgccact ggcagtccgt ttaatccagt ggtatggaaa gataaaatct
     2101 accctatagc ccagtgcaac aacgccttta ttttcccggg catcgggctg ggtgttattg
     2161 cttccggcgc gtcacgtatc accgatgaga tgctgatgtc ggcaagtgaa acgcttgctc
     2221 agtattcgcc gctggtcctg aacggcgaag gtctggtact accggaactg aaagatattc
     2281 agaaagtctc ccgcgcaatt gcctttgcgg ttggcaaaat ggcgcagcag caaggcgtgg
     2341 cggtgaaaac gtctgccgaa gctctgcaac aggccattga tgataatttc tggcacgccg
     2401 aataccgcga ctaccgccgt acctccatct aagcctgcgc ccggtagtga aggctaccgg
     2461 gcaattttcc tctccctttt tcagatctca tccatactgg gtagtggcga ataaatctca
     2521 tttgcctcac ctgctatgca gaacatcatc cgaaaaggag gaactatgaa ggctgcagtt
     2581 gttacgaagg atcatcatgt tgacgttacg gataaaacac tgcgctcact gaaacatggc
     2641 gaagccctgc tgaaaatgga gtgttgtggt gtatgtcata ccgatcttca tgttaagaat
     2701 ggcgattttg gtgacaaaac cggcgtgatt ctgggacacg aaggtattgg tgtggtggca
     2761 gaagttggcc ctggtgttac ttcattaaaa ccaggcgatc gtgccagcgt ggcgtggttc
     2821 tatgaaggat gcggtcattg cgaatactgt aacagtggta acgaaacgct ctgccgttca
     2881 gttaaaaatg ccggatacag cgttgatggc gggatggcgg aagagtgcat cgtggtcgcc
     2941 gattacgcgg taaaagtgcc agatggtctg gactcggcgg cggccagcag cattacctgt
     3001 gcgggggtca ctacctacaa agccgttaag ctgtcaaaaa ttcgtccagg acagtggatt
     3061 gctatctacg gtcttggcgg tctgggtaac ctcgccctgc aatacgcgaa gaatgtcttt
     3121 aacgccaaag tgatcgccat tgatgtcaat gatgaacagt taaaactggc aaccgaaatg
     3181 ggtgcagatt tagcgattaa ctcacgcacc gaagacgccg ccagaattgt gcaggagaaa
     3241 gccggtggcg ctcacgcagc ggtggtgaca gcggtagcta aagctgcgtt taactcggcg
     3301 gtggatgctg tccgtgcagg tggtcgtgtc gtggctgtcg gtctgccgcc ggagtctatg
     3361 agcctggaca tcccacggct ggtgctggat ggtattgaag tggtcggttc gctggtcggc
     3421 acgcgtcagg atctaaccga agccttccag tttgccgccg aaggcaaagt ggtgccgaaa
     3481 gtcgccctgc gtccgttagc ggacatcaac accatcttta ccgagatgga agaaggcaaa
     3541 atccgtggcc gtatggtgat tgatttccgt cgctaagagg tctttgctgc gactgccatg
     3601 ttcgggtcgc agcctgtttt gcgagacgct ttccgcaatt tcactcttct tcttttcctc
     3661 ccccatcttt cccctcacaa ttcgcgaaaa cgtagcgaaa tttgttgcgc tgtacggaac
     3721 aatagtgata gtgttccttt cgagggaaca aggatgaaga gatgataatg aatttcagac
     3781 acaagggatt gcgtgacttg tttcttctcg gcaaaacctc cggcgttata ccgacacgat
     3841 tacgccaccg actcgctgtg attgatgcag catgctgtct tgctgatatc gatatgcccg
     3901 gttaccgact acatccgtta agcggcgatc gcgatggaat ttgggcgata tctgtctcgg
     3961 gcaactggcg aatcacattt gaattcgtca atggcgatgc atacatactg gattacgagg
     4021 actatcactg atgaaaatgg ccaatcatcc ccgcccgggg gacattattc aggaatcact
     4081 ggacgaactt aatgtcagcc tgcgcgagtt tgccagagca atggaaattg cgccctcaac
     4141 ggcaagccga ttgctgaccg gaaaagcagc tttgacgcca gaaatggcaa taaaactttc
     4201 cgtggtgatc ggcagttcgc cgcaaatgtg gctgaatctg caaaatgcct ggagtctggc
     4261 agaggcagaa aaaacagtgg atgtgtcgcg actgcgccgt ctggttacgc aataaaaaaa
     4321 tggcacctta taaagcgcca tttgaaactg atgacaaacg caacattgcc cgatgcgcta
     4381 cgcttatcag gcctacacgt tcactgcaat ctattggatt tgaacggttt gtaggccgga
     4441 taaggcgttc acgccgcatc cggcatgaac aaagtgcacg ttgtctacaa taaaaaaatg
     4501 gcgcctttta cagcgccatt ttcagcttaa agctcaaaag agattataac ccttcttcac
     4561 tctctttttt cgcttctgcc ttctcgattt cacgatacca gcgcggatgg tgtttcttcg
     4621 cccagcgacg gctcaccttc ccttcgatca tccctttaat agatcctttc acccagaatg
     4681 ccatatacat atggatcagg atggcgtgga tcaggatgat acccgcagcc gcatggatca
     4741 gcaggctata acggacaacc tgtattggga agtactgcgc aaagtacgga cgccagataa
     4801 tcactccggt caccagcagc acgaaaatca tgctcatgat tgaccagaac atcatctttt
     4861 gcccggcgtt gtacttaccg acatccgcca ctttgtgctc attgcctttt aatacttcga
     4921 caatgttcaa cagccacgga atatctttct tatccgggat gttgtgatgc acaaaacgca
     4981 caaacataaa catcagcgcg acgaaaatcg caatgccgaa gaacgggtgc aaaatgcgtc
     5041 ccatctgcgg cgtaccgaag gtttgcgtca gccattgcag cgtcgggaag aagaacgaaa
     5101 tcccggacag cgccaccagg aagaagcaaa tcaccacggt ccagtgacag gcgcgatcaa
     5161 taaatttggt gcgcacaatc attttcgact tactcatgat ttccctcctc gtcatcgtcc
     5221 acttccttat tcgggccaat accgatgtag tggaaaatca acccggcaaa agtggcaata
     5281 aagccagccg ctgccagcgg tttcaacgcg cctttccaca ggcttaccga ggtatcgatc
     5341 ttcggatctt tcggcagacc atgatacagc tccggctgat cggcatgatg cagcacgtac
     5401 ataacgtgtg taccaccgac cccttccggg ttgtagacgc cagcatgttc gtaaccacgc
     5461 gctttcagtt tcgctacgcg ctgttccgcc agctccagca tctccttctt ggtgccgaag
     5521 tggatagccc cggtcggaca ggttttcaca caagccggtt cctggccgac gctgacgcga
     5581 tcgacgcaga gcgtgcattt atatacccgg ttatcctctt tgttgaggcg cggaatatta
     5641 aacggacacc cggcaatgca gtaaccacag ccgatgcaat tttccgactg gaaatcgaca
     5701 atcccgttag cgtactgaat gattgcacca gcagacgggc acgccttcag gcagcccgga
     5761 tcttcacagt gcatacagcc gtctttacgg atcagccact ccagcttgcc gttctgttcg
     5821 gtttcgctaa agcgcatcac cgtccaggac ttggcgctca gatcggcggg gttatcgtaa
     5881 accccgacgc agtgccccac ttcatcacgg atgtcgttcc actccgaaca cgccacctga
     5941 caggctttac agccgataca ggtggaaacg tcgataagtt tcgctacttc tgctttgtaa
     6001 tcacgcacct gagaaggcgg cgtgatggag ttagttgcgg accttttgat aatgtcctgc
     6061 gtttccatag ccatctgttc gcccccttac gccttctcga tgttgactaa gaacgcttta
     6121 tattccggcg tttgcgagtt tgcatcaccg acattcggcg tcagagtgtt agcgatataa
     6181 cctttacgcg cgacaccctc aaagccccag tggattggaa tacccaccgt ttcaacctgc
     6241 tgaccgttta cattcagcgg tttcagacgg cgcgttacca cagccaccgc gcggataaag
     6301 ccacgcttgc tggagacagt gacacgatcg ccattggcaa tgcctttcgc cgccgccagc
     6361 gtttcgctga tttccacaaa ctgttccggc tgagcaattg cgttgagcaa tgcgtgcttg
     6421 gtccaggtgt ggaagtgctc cgtcagacga taggtcgtac ccacatacgg gaactgctct
     6481 tttttaccca tccgcagcgc gtcttgttca tacagacgaa caaccgggtt agacaccacg
     6541 ttcggatgca acgggttagt gcccagcggc gtttcaattg gctcgtagtg ttccgggaac
     6601 ggaccttccg ccattttgtt gatggcaaac aggcgtccca tcccttccgg ctgcatgata
     6661 aacggcccgg ttggcgtacc cggtgcggca ttgccgaagt ccggaatatc gttacccgtc
     6721 cacttgctac cgttccactg gatcagcatc cgtttcggat cccacggttt accgttgata
     6781 tccgccgaag cacggttgta gagtacgcga cggttgagcg gccacgccca ggcccatccc
     6841 agcgtattcc ccagaccgga cgggtcggag ttatcgcggt tagccatctg gttgccctgc
     6901 tcggtccagc taccggtgta gatccagcaa gaagatgcgg ttgtaccgtc gtcacgcaga
     6961 tgcgcaaagc tactcagcaa ctgacctttc ttcgcaatca gcacgccatt agcgtcatag
     7021 agatcttcca gcgcatagcc gttgttctct ttcgccactt cgtcagactg cggttcgtgc
     7081 ggctgcttgt agttccagct catcttcatc agcggttcta cgcctttacc accctcggcc
     7141 tggtacagct cgcgcagatg atggtagata cccgccagaa tttcgccgtc gttacgtgct
     7201 tcgcccggcg cgtcctgacc tttccagtgc cactgcaacc agcgaccgga gttagcgata
     7261 gaaccatctt cttcagcaaa gcaggtcgaa ggcagacgga atacttcagt ctgaatagat
     7321 gccggatcga catcgttcga ctcgccgtgg ttctgccaga aggtagaggt ttcagtcacc
     7381 agcggatcga taaccaccat gtacttcagt ttgctcaggc agctcaccac tttgtttttg
     7441 tccgggaagg acgcaaccgg gttaaagccc tggcagaaat aaccggtgac tttgccttcg
     7501 tccatcatgt tgaaatactt gatgacgtcg taggtctggt cccacttcgg cagccagtcg
     7561 tagccccagt tgttctcttt ctgcgcggca tcgccgtaga aagatttcat caggctaacg
     7621 aagaacttcg gatagttgct ccagtagttc acctgatcag ccagcgtcgc tttcggcgta
     7681 ttcgcttcca gatacgactg caaatcaacc tgtttttctg acggtagcgt cagataacct
     7741 ggcaggctgg tagagagcag gcctaagtca gtcaggccct gaatgttgga gtgaccacgc
     7801 aatgcgttca cgccgccacc ggccataccc atgttaccga gcagcaactg gatcatcgcc
     7861 atcgtacgga tgttctgcgc acccacggtg tgctgcgtcc agcccagcgc gtacaggaag
     7921 gtggttgtgc gatccggtgc gctggtggag gccagcactt cacacacttt caggaagtcg
     7981 gcttttggcg taccgcaaat gttttctact acgtccggcg tgtagcggga aacgtgcgct
     8041 ttcagcagat tccatacaca gcgcggatga gtcagcgtgt catcgcgttt cgcatagccg
     8101 ttttcatcga actgatagtt ccaggacgac ttatcgtatt gacgtttttc agcgtcgtag
     8161 ccgctgaaca gaccgtcttc gaaagcaaaa tcatcacgca ccagcaggct ggcgttggtg
     8221 taatgcttaa cgtattcggc gttgattttg ttgttttcga tcaggtagcg caaaacgcca
     8281 gacaggaacg taatgtccgt accggaacga ataggtgcgt aaatatccgc cacagaagcg
     8341 gtacgcgtaa aacggggatc gacaacgatc aaggttgcat cgttgttgtt tttcgcttcc
     8401 atcgcccagc ggaaaccgac gggatgcgct tcagcagcgt taccgcccat caccattacg
     8461 acgttagcgt ttttgatatc cacccagtgg ttggtcatcg caccgcgacc aaatgttgga
     8521 gcaagacttg ctaccgttgg tccgtgtcag acgcgcgcct ggttgtctac cgccagcatc
     8581 ccgagggagc gggcaaattt ttgagtcagc atcccggttt cgttgctggc accggaagca
     8641 cacagcatac cggtagaaag ccaacggttt accgttacgc cctgctcgtt cttttcaata
     8701 aagttagcgt cacggtcggc tttcatcagc ttcgcaatac gggagaatgc ttcttcccag
     8761 ctaatgcgct gccatttgtc agaacctggc gcacgatatt ccgggtagcg cagacggttt
     8821 tcgctgttga cgtaatccag caaaccggcc cctttcgggc acagcgcacc acggcttacc
     8881 ggatgatccg ggtcaccttc aatgtgataa atcgcttctc tggcgttttt cgcgccatca
     8941 cccaggctat acatcaatag cccgcaacct acggaacagt atgtgcaggt gttacggatc
     9001 tctttcgcgc gtaataattt gtagtttcgc gcctgagcca gtgcttgctt cggggcaaag
     9061 cccaatgctg ctactgttgt tcccgccata ccgcccgcgc agattttaaa aaattgtctg
     9121 cgactgacgt ccattgcttt cctctttttt caggggtatt actgcgcgag gaaactaaca
     9181 gtaaaattct gaatcttttt gcgcgaaatc aaaaagaaca cgtgtttgcc acaaaataat
     9241 ccgcctgcgc aaattgacct acctcaatag cggtagaaaa acgccccact gcctgacagg
     9301 gcagttaaaa aaatgctata aaattcagct taatttttaa cggcaagaga gacaaaacag
     9361 cgagcatgac acgacaaaaa gcaacgctca tagggctaat agcgatcgtc ctgtggagca
     9421 cgatggtagg attgattcgc ggtgtcagtg agggactcgg cccggtcggc ggcgcagctg
     9481 ctatctattc attaagcggg ctgctgttaa tcttcacggt tggatttccg cgtattcggc
     9541 agatcccgaa aggctattta ctcgccggga gtctgttatt cgtcagctat gaaatctgtc
     9601 tggcgctttc cttagggtat gcggcgaccc gtcatcaagc gattgaagtg gggatggtga
     9661 actatctatg gcccagcctg acaattctct ttgccattct gtttaatggt cagaaaacca
     9721 actggctgat tgtaccagga ttattattag ccctggtcgg cgtctgctgg gtgttaggcg
     9781 gtgacaatgg gttacattat gatcaaatta ttaataatat caccaccagc ccattgagtt
     9841 atttcctggc gttcattggg gcgtttatct gggcagccta ctgcacagta acgaataaat
     9901 acgcacgcgg atttaacgga attaccgttt ttgtcctgct aacgggagca agtctgtggg
     9961 tttactattt tcttacgcca caaccagaaa tggtatttag cacgcccgtc atgattaaac
    10021 tcatctctgc ggcatttacc ttaggatttg cttatgctgc atggaatgtc ggtatattac
    10081 atggcaacgt caccataatg gcggtaggtt cgtattttac gcccgtcctt tcctcagcgc
    10141 ttgcagccgt gctgctcagc gccccgctgt cgttctcgtt ctggcaaggc gcgctaatgg
    10201 tctgcggcgg ttccctgctc tgctggctgg cgacacgtcg tggctaaaat aattgtcggg
    10261 tctacggccc ggcagttaat cagataaatt ctgacaatat taaaaa
//
LOCUS       DADSZD010000153        10298 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.154, whole genome shotgun sequence.
ACCESSION   DADSZD010000153 DADSZD010000000
VERSION     DADSZD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.154"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..1810)
                     /gene="selB"
                     /locus_tag="J1Z28_003568"
     CDS             complement(<1..1810)
                     /gene="selB"
                     /locus_tag="J1Z28_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBA7718369.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRIVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDH"
     gene            complement(1807..3198)
                     /gene="selA"
                     /locus_tag="J1Z28_003569"
     CDS             complement(1807..3198)
                     /gene="selA"
                     /locus_tag="J1Z28_003569"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBA7718370.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAANASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHAKDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3296..3904)
                     /locus_tag="J1Z28_003570"
     CDS             complement(3296..3904)
                     /locus_tag="J1Z28_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBA7718371.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AIGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3975..5111)
                     /locus_tag="J1Z28_003571"
     CDS             complement(3975..5111)
                     /locus_tag="J1Z28_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7718372.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5114..5476)
                     /locus_tag="J1Z28_003572"
     CDS             complement(5114..5476)
                     /locus_tag="J1Z28_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBA7718373.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVTPQEKTTDAEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6013..7926
                     /locus_tag="J1Z28_003573"
     CDS             6013..7926
                     /locus_tag="J1Z28_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBA7718374.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8245..9393
                     /gene="mtlD"
                     /locus_tag="J1Z28_003574"
     CDS             8245..9393
                     /gene="mtlD"
                     /locus_tag="J1Z28_003574"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA7718375.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9393..9980
                     /gene="mtlR"
                     /locus_tag="J1Z28_003575"
     CDS             9393..9980
                     /gene="mtlR"
                     /locus_tag="J1Z28_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor MtlR"
                     /protein_id="HBA7718376.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(9991..10200)
                     /locus_tag="J1Z28_003576"
     CDS             complement(9991..10200)
                     /locus_tag="J1Z28_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718377.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
BASE COUNT         2424 a         2817 c         2670 g         2387 t
ORIGIN      
        1 aatgatcatt tccacgacga cgcgtaaagc caatgcggtc aaaatattcc agaatctgaa
       61 ttgccagctt gcggcctacg cctaagcgat cgcggaaatc cgccgcgcag gttgaaccgc
      121 attcctgatc gagatcgcgg atcatattgg caaattcgac aatccgatcg ttacggtaat
      181 aacgatcttt gacgatcgca gtaattatcc cctgttgcgc cgcctggcgt agcgtcaggc
      241 gcattgcctg ctcgtcggtt cccgtctctt ttgccaggtc acgcacccac catggctcgt
      301 caccaaacag cggctctgct ttttgccaga tggcctgctg ctctgcactg aagcccgctt
      361 tatgatccgg cagatgcagc cagccgtgat gactgtggat gtcgccgctt tcgcgcatct
      421 tttcaatcag caacagtacc agcgcttcat cttccattgg caacgccata cgtcgcagac
      481 gttcgcgccc aggaccaggt tcatcgcgat gttgctcatg ataagtcgct aatgtgtcga
      541 gaattttccg ctgccagcgg gcggcaactg gcgcattcaa caagctgtaa ccagcctgaa
      601 tatagccagg ctgttgcagc agttcgcgca tcccttcgcc gttgagctgc cgtgcccagg
      661 cgaaatccgc aaggttaacc gcgccgcgtt ccagatgaac agataacgca tcggcatcgc
      721 tctgcgcccg tgcaagagac gccagccatt gcagatattc cggcttacgc ttaccgcgac
      781 gcggcgggtt aagcatcacc acacgcgccc ccgccagcgt gttgcgggcc gagatatcgc
      841 gcagcactat gcgatcgtta tccgccagcc acagcggcgt gtcgaacacc agctcggcaa
      901 ggttatcttc cagcagcgaa acgcgtcccg taacgtggct ggcggcgtgg tgaatatgca
      961 gcggctgcca ctgggtcagc ggtgtatggg tttgaagctc gacaatcacc cgtgtgaacg
     1021 gctccggcgg cacatcggca agcagccagt cgccacggtt aatctgctct ttttccgcat
     1081 cacccgcgat gttaagcgcg atacgctgcc cggcatgggc agtttctgtt ggctgatttt
     1141 gcgcatgcag cgcacgcacg cgcatcggtt tattcacacc agtcagccag agtgaatcgc
     1201 ctactttcac ttccccgctt aacgccgtac cggtgacgac cagcccggca ccttttacgg
     1261 taaatgcgcg gtcgatcgcg aggcggaaac tatgttggct ggcgtgttca cgttctggta
     1321 actgaagcag atgctcgcgc agggcatcaa ttccccgacc ttcggttgct gcggtgataa
     1381 acagttttgc ctcagcaaaa ccgtattccc gcagcacctc ctttacctgg cgttcaacct
     1441 catcaacacg cgcttcgtcc acgcgatcgg ctttggtcag cgccactgtc agcatcgggt
     1501 taccggtcag ctgcaaaatc gccagatgct cacgggtctg tgccatcaca ccgtcatcgc
     1561 acgccaccac caacagcgcg tgatcgatac caccaacgcc cgccagcatg ttggaaagaa
     1621 acttttcatg accgggtacg tcgataaaac caggcacgcg accatccggc tgcggccagt
     1681 aggcatagcc gagatcgatg gtcatgccgc gctttttttc ttccggcaga cggtcggcat
     1741 ttacgcccgt aatcgcctgc aacaaggttg ttttgccgtg gtcaacgtgt ccggcagtcg
     1801 caataatcat ttcaacaaca tctccaaaaa ccgttgctca tcttcaaggc agcgtaaatc
     1861 cagccacaat cgtccgtcat aaatacgacc aatcaccggc actggcaatt cacgccagcg
     1921 ggcggctaat gactcaaggt ggctaccgcg tccatcatgg ggggtaaacg ttaatgccgc
     1981 gctcggcagg cgatcaaccg gcagcgaacc actgccgatc tgcgaaagac atggcataac
     2041 ctgtaccgca aactccgcgc cgtaatgtgc ggcaagggga gcctgtaaac gttgtgcctg
     2101 gatttgaatg acctctgcgc tgcgggtaag caggcgcagg gtcggtaatt tttcactcag
     2161 agcttcaggg tgtaaataaa gacgcaacgt ggcttccagc gccgcgaggg tcattttatc
     2221 cgcgcgtaat gcacgcttca gcgggtggct ttgcaggcgg gcgatcattt cttttttacc
     2281 aacaataatt cctgcctgcg gcccgcctaa cagcttgtcg ccggagaagc tcaccagact
     2341 gacgcccgcc gcaatcaact cctgcggcat tggctctttc ggcaaaccgt actggctaag
     2401 atcgaccagc gagccactgc ctaaatcagt caccactgga acatccagtt ctttgccgag
     2461 cgccaccagt tccgcttcat ctatcgcttt ggtgaacccc tgaatgctgt agttactggt
     2521 atgtactttc atcaacagtg cggtattttc attcaccgcc tgacgataat ccttcgcgtg
     2581 cgtgcggttg gtggtcccta cttcgtgtag ggtgcatcct gcctgacgca taacatcggg
     2641 aatacgaaac gcgccgccaa tctccaccag ttcgccgcga gaaaccacca cctctttccc
     2701 gctggcattg gccgccaaca tcaataacac cgccgccgca ttgttattaa caatacaggc
     2761 atcttccgcc cccgtaatac ggcacagcag ctgcgccagc gcccgatcgc gatgtccgcg
     2821 tccggcgtcg tccagatcgt actcgagggt cactggcgaa cgcatagcct tcgtaacggc
     2881 ttccaccgcg gcttccgcct gtaaagctcg cccaaggttg gtatgcagca cggttcccgt
     2941 caggttgatc accggacgca gcgcgctttg cgcctctttc gtcaaccggg catcgacttc
     3001 ttgcgcccag ttttcacacc acgcgggcag cgtctggctg tcacgaatca cttctcgcgc
     3061 ttcgtcaagc atctgacgca gcaattccac cacgcgggtg tgaccataag tatcacgcaa
     3121 agaaaggaag gagctatcgc gcaataagcg atcaatagcc ggaagttgac tatagaggga
     3181 acgcgtttcg gttgtcatag gaaacctggc tgatcaaggc cctctcacac ggagaagggc
     3241 gtttaacata accacggatt gtaacgtgag atgggtcagg aggacatatc gcgcatcaag
     3301 cctttggcgg ttcggtgcga gcaaaacttt cgcggctaaa caggttttcg accagtttga
     3361 ctaagtgtgg gcggtcgaca caccagcctg gcgcaacgcg gcggaaattg agatatccga
     3421 tggcacaggc aatggcgata gtcgccagat tgaccgtatc ggttttgagt gtgccatcga
     3481 ccagatatcc ttccagcaca tccagactgc ggttgatttt ctcacgctga cgtaacaatt
     3541 catcttcaga ctgctgcgcc gccggacgcg cctgttcacg caccgatacc agcccggcat
     3601 ccataatgcc atccgccagt gcctcaattt tgcgcacccg caacgactcc agcggatcgc
     3661 gcggcaacat cgccggagca acattcatta attcaatata ttcagcgatg atcggtgaat
     3721 caaaccagca ttcgccctct tcggtcacca gtaccggtac ttttcctaac gggttaaatt
     3781 gcgccacgcc gttatccgcg ttatagggca gttcattaat aaattcgaac gttatgccct
     3841 tttctaacaa cagaatagaa agtttgcgta caaacgggct ggtgtagcta ccgacgagtt
     3901 tcatgccgag tcctttgtgc gaggaaaaat atcagtatgg ctcaggctcg cgcaaaacac
     3961 aggtgataca agtctcaatg atccaaataa agataatgca tccaactggt cattcttagc
     4021 agcactttcc gcatcaccga aacatggctg aaatggtcgg agtaaactgc cacctgggcg
     4081 tagatgccgt cgggtaaggc atcgatatca gcgttagggt ccagttcaat ggttcccagc
     4141 acaccgtccg tgccgggcac gaccgttaat gattgcaata ccccctgcgc ctgataagaa
     4201 ccgcctggca cgacaggtaa aatactggtc agtttgccgt gaaacacctg cccaggtagc
     4261 gcgttaaaca ccacttccgc atcatcgccg ggtttcagac gtaacagcga gttttgccga
     4321 aattgggcga caatttgccg tttttgctcg gggatgaaga ccatcaccgg acgcagcggc
     4381 aaggcagctg cgtatgtacc tgggcggatc agtacctgag tgacgtagcc attgctcggt
     4441 gcgcgaatga cagtctgctc aaggttatat tttgcttcag taagttgcgc tcttaagctc
     4501 acaatctgcg attgctcgcc gttaaccata ctgtcgagct ggctctggat ctgcgcctgc
     4561 tccgccaccg agcctttcac cagcgcatcc tgcgcgagga aattttgccg cgcatcgtcg
     4621 atgtcacgtt ccgagaacgg attcaccgcc gcctggctgc ctttcagata acgttgataa
     4681 tttttaaaca gacggtcgcg ctccgctgaa acctgggtgg tgttggcctg cgcttcagtg
     4741 agctgcgcgc gcagcgtctt tatattatgc gtcgccgtca tcaggtcagc ctgaagcctg
     4801 tcaactcgcg cctggtaacg aaccgggtcg agcttaaaaa gcacctcgcc cttctgaata
     4861 agctgattat ttttgtcggt gacttcagta acaattcccg tcacctgtgg cgtgatgggg
     4921 atcgctatca ctgccttttg cgcggtaaaa gtgtaagggt ggttgtagtt catcaacaaa
     4981 atcaaaccac tcaccagaaa cacgcctccc agcgccgccg tcgccagcgt ccactgattc
     5041 accggaatgc ggaagatttt aaagaccgcc cacgccagcg ccacgtaagt taaaacaatc
     5101 aatagatcca taattagatc tccggaaacg tggaatagtc agtctttttc tcggcggcga
     5161 gttggtgctc cagccgggaa atccggtcag aaagtgcggc gatttccgcg tcagtcgttt
     5221 tctcttgcgg cgtaacatgc gactgcatgc cccagccgcg ctccggttga tagagcgtcg
     5281 cccagatcca caggaacggc cagataacat gcagagtaaa caggctcacc cagcccgccg
     5341 tatgaatagc gtcggcatgg ggatggttgc gctttttggc aatcagataa gggatgtcat
     5401 gtatcgcgat gattccataa aaaatcacca gaaagacgaa gatcagcact cccaacgcga
     5461 aatagtttag aaacatatct gcctcggatt cacgtttatc agtgttgttt tttggctggc
     5521 agccagaagg gagtcaggct gatattttga caataatccg ggttcgcgat tctcgccata
     5581 acaccaaaga ataattttaa gaggtgatga gttgcttagt tacataacga tttcatgacg
     5641 aaggcataac atgctgtaga tcacatcagg tgaacgccgt aagaaaatat cttgtgattc
     5701 agatcacaaa gattcaacaa accatcaaaa taaaaatgtg acataactca catttaaatg
     5761 ccatttttag cgaaaatcgc cgccttgttg cttttttaca caagcatttt gtgatgagca
     5821 tcacgtcaat tacctctcta ccccctacat ttatgtgact gatatcacac aaaaggccgc
     5881 cgactggaca gttaaccgat tcagtgccag atttcgcagt atctacaagg tccggctacc
     5941 tctgccgcca cattaacaaa aaacctcggg cttccagcct gcgcgacagc aaacataaga
     6001 aggggtgttt ttatgtcatc cgatattaag atcaaagtgc aaagctttgg tcgtttcctc
     6061 agcaacatgg tgatgccaaa tatcggcgcg tttatcgcgt ggggtatcat caccgcgtta
     6121 tttattccaa cagggtggtt accgaacgaa acgctggcga agctggtcgg gccgatgatc
     6181 acttatctcc tgccgctgct gatcggttat accggtggta agctggttgg cggcgaacgt
     6241 ggcggcgtag tcggtgccat caccaccatg ggcgttatcg tcggcgcaga catgccgatg
     6301 ttcctcggtt ctatgattgc aggtccgctg ggcggctggt gcattaagca cttcgaccgc
     6361 tgggtagacg gtaagatcaa atccggtttt gaaatgctgg tgaataactt ctccgcaggc
     6421 atcatcggga tgatcctcgc tattctggca tttctcggca ttggcccggt tgttgaagcc
     6481 ctgtctaaaa tgctggcggc gggcgttaac ttcatggttg tccatgacat gctgccgctg
     6541 gcgtctatct ttgttgaacc ggcgaagatc ctgttcctca acaacgctat taaccacggt
     6601 atcttctcgc cgctgggtat tcagcagtcc catgaactgg gtaaatccat cttcttcctg
     6661 attgaagcta acccaggtcc gggtatgggc gtgctgctgg cgtacatgtt ctttggtcgt
     6721 ggtagcgcta aacagtctgc gggcggtgcg gcaatcatcc acttcctggg cggtatccac
     6781 gaaatctact tcccgtatgt gctgatgaat ccgcgtctga ttctggccgt tatcctcggt
     6841 ggtatgactg gcgtgtttac gctgactatc ctgggcggtg gtctggtttc tccggcatct
     6901 ccgggttcta tccttgctgt actggcgatg acgccaaaag gtgcttactt cgctaacatc
     6961 gcgggtgtgt gtgcggcgat ggcagtctcc ttcgttgtct ctgccattct gctgaaaacc
     7021 agcaaagtga aagaagaaga tgatattgaa gctgctgcac gtcgcgttca ggaaatgaaa
     7081 gctgagtcta aaggcgcatc tccgctgtct gcgggcgatg tgactaacga cctgagccac
     7141 gtacgtaaaa tcatcgttgc ctgtgatgcc ggtatgggtt ccagtgcgat gggcgcaggc
     7201 gtgctgcgta agaaaattca ggatgcaggt ctgtcgcaga tttcggtcac taacagcgcg
     7261 atcaacaacc tgccgccaga tgtggacctg gtcatcactc accgtgacct gaccgaacgc
     7321 gctatgcgcc aggttccgca ggcacagcat atttcgctga ccaacttcct cgacagcggc
     7381 ctgtacacca gcctgaccga acgtctggtt gctgcccagc gccatactga aaacgaagtg
     7441 aaagtgaaag acagtctgaa agacagcttt gacgattcca gtgctaatct gttcaaactg
     7501 ggcgcggaga acatcttcct cggtcgcaaa gcggccacca aagaagaagc gattcgtttt
     7561 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     7621 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     7681 gttgaagcga aagatcgcgt gctgaaaacg ggtgtggtgt tctgccagta tccggaaggc
     7741 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgctgcccgt
     7801 aacaacgagc acattcaggt gatcaccagc ctgaccaacg cactggatga tgagtccgtc
     7861 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaattgct ggcaggtcgt
     7921 aagtaatcca ttcccaccct ctccgcatga agaaggtggg gctaattgcc tgatgcgctt
     7981 cgcttatcag gcctacaaca taaagcacaa tttgttttgt cggtcggata aggtatttac
     8041 gccgcatccg gcagtcggtg cacaatgcct gatgcgacgc tgacgcgtct tatcaggcct
     8101 acaggtcagt gccaaacacg taggccggat aaggcgttta cgccgcatcc ggcaaaaagc
     8161 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
     8221 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
     8281 cgtggcttta tcggtaaact gctggcggac gcgggtatcc aactgacgtt tgccgatgta
     8341 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggc
     8401 gaaaccgagc aggtagatac cgtttccggc gtcaatgccg tcagcagcat tggcgatgat
     8461 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc tgtcgtgctg
     8521 gaacgtattg ctccggccat cgccaaaggg ctggtgaaac gtaaagaaca aggtaatgaa
     8581 tctccgctga acatcatcgc ctgtgaaaac atggtccgcg gcaccacgca gctgaaaggc
     8641 catgtgatga acgccctgcc ggaagatgcc aaagcgtggg tagaagaaca cgttggcttt
     8701 gttgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
     8761 gaagtgacgg tagaaacctt cagcgaatgg attgtcgata aaacgcagtt caaaggcacg
     8821 ctgccaaaca tccctggcat ggaattaacc gacaatctga tggcatttgt cgaacgtaaa
     8881 ctcttcaccc tgaatacagg tcatgctata accgcgtacc tcggaaaact ggccggtcat
     8941 cagaccattc gtgacgcaat tctcgacgag aaaatccgcg cggtggtaaa aggggcaatg
     9001 gaagaaagcg gcgcggtact gatcaagcgc tacggctttg atgctgacaa acatgcggca
     9061 tacatccaga aaatcctcgg tcgtttcgag aacccgtacc tgaaagatga tgtagaacgc
     9121 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
     9181 ggaaccctgg aatatggtct gccacataaa aacctgattg aaggtattgc cgctgcaatg
     9241 cacttccgca gtgaagatga cccgcaggct caggaactgg cagcgctgat tgccgacaaa
     9301 ggcccacaag ccgcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
     9361 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gataccctgc
     9421 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gcctttgaaa
     9481 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
     9541 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
     9601 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga tggtccgctg ggcgatcttt
     9661 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
     9721 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgctttta
     9781 ccgacgacga aatacttgga ccctttggtg aactgcattg cgtggcggcg ttaccaccgc
     9841 cgccacagtt tgaacctgca gactccagtt tgtatgcaat gcaaattcag cgttaccaac
     9901 aggctgtacg atcaacaatg gtcctttcac tgacagagct gatttcaaaa atcagcttaa
     9961 aaaaagcctt tcaaaagtaa gtactttcgc ttacttctcc tctacctgtt tcggacgata
    10021 aagctgacga taatactcca gacgttgtag atataacggc acgctttcat caggtattcc
    10081 ggacggaatc gcgttacggg gaggtagttt tttcagatac tcccggaacg cctggctgga
    10141 tgccatgaaa tctacggctt tatcgataag aagcggaacg ttttcaccta atttgcccat
    10201 gatgatatct ccgtattacc ccgcgccggg aatgcgcggc cgccaatttt agtttagatc
    10261 ccgatataaa tgacattaag gaaaacgtgc tcaatcct
//
LOCUS       DADSZD010000154        10249 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.155, whole genome shotgun sequence.
ACCESSION   DADSZD010000154 DADSZD010000000
VERSION     DADSZD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.155"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(96..869)
                     /gene="uxuR"
                     /locus_tag="J1Z28_003577"
     CDS             complement(96..869)
                     /gene="uxuR"
                     /locus_tag="J1Z28_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBA7718378.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1084..2544)
                     /locus_tag="J1Z28_003578"
     CDS             complement(1084..2544)
                     /locus_tag="J1Z28_003578"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBA7718379.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLEITDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2625..3809)
                     /gene="uxuA"
                     /locus_tag="J1Z28_003579"
     CDS             complement(2625..3809)
                     /gene="uxuA"
                     /locus_tag="J1Z28_003579"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBA7718380.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4149..5492
                     /gene="gntP"
                     /locus_tag="J1Z28_003580"
     CDS             4149..5492
                     /gene="gntP"
                     /locus_tag="J1Z28_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBA7718381.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQI
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5735..6637)
                     /gene="fimH"
                     /locus_tag="J1Z28_003581"
     CDS             complement(5735..6637)
                     /gene="fimH"
                     /locus_tag="J1Z28_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBA7718382.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSSFSGTVKYNGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6657..7160)
                     /gene="fimG"
                     /locus_tag="J1Z28_003582"
     CDS             complement(6657..7160)
                     /gene="fimG"
                     /locus_tag="J1Z28_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBA7718383.1"
                     /translation="MKWCKRGYLLAAMLAFASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSLSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(7173..7703)
                     /gene="fimF"
                     /locus_tag="J1Z28_003583"
     CDS             complement(7173..7703)
                     /gene="fimF"
                     /locus_tag="J1Z28_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBA7718384.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNEIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7713..>10249)
                     /gene="fimD"
                     /locus_tag="J1Z28_003584"
     CDS             complement(7713..>10249)
                     /gene="fimD"
                     /locus_tag="J1Z28_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein FimD"
                     /protein_id="HBA7718385.1"
                     /translation="AFAAQAPLSSAELYFNPRFLADDPQAVADLSRFENGQELPPGTY
                     RVDIYLNNGYMATRDVTFNTGDSEQGIVPCLTRAQLASMGLNTASVSGMNLLADDACV
                     PLTSMIHDATAQLDVGQQRLNLTIPQAFMSNRARGYIPPELWDPGINAGLLNYNFSGN
                     SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLER
                     DIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQ
                     VTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLL
                     QREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFG
                     IGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTS
                     GYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTST
                     LYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNI
                     PFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGG
                     GDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLND
                     TVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTLADNVDLDNA
                     VANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQV
                     YLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2390 a         2736 c         2569 g         2554 t
ORIGIN      
        1 ttaatttacg cataatatat tctgcagaac ataattaact attcagggaa catacatagt
       61 gcaaagcggt taaactttgc accagaaatg agggatcagg cgtcgacttt atccagcggc
      121 catgaatcaa acagatagcc atcgaaatag atgtcatcaa cattagagaa ctccagcaag
      181 cgctgcttca cattctccag atgctgccac attgccagct tcgccgctcg ggcatctttt
      241 ttgattaacg cggcgaggat ctgtttgtga tcgcccaacc actctttgcg atacaggctg
      301 tcatccaggt ggctgtgcaa ctggatccac attggattgt tttcccgcca ctgccaggac
      361 tgacggaata actccaccag catgctgtta tgcgttgctt cggcaatagc gagatggaac
      421 tgcatgtcac cgctttcgct gctgcccggc gcgctggaag ccagttcacg ctcttccagt
      481 tgcaatgcct ggcgcatttt gacgatatct tcgcgggtag cctgcagggc ggcaaactcg
      541 gcgatgttac tttccagtaa ctgccgcgcc tgtaacagct caaaaggacc ggcatcgttg
      601 cagacgttgg catccggact gtctgcgttg tggctgcctg agctatcaag aacatagata
      661 cctgcacccc gacgcacttc caccagccct ttgatctcca gcatgatcag cgcttcacgt
      721 accaccgtcc gcgtgacatc aagcatttct gcaatttcac gctccggtgg cagccgttcg
      781 ccaggattgt acggcgtctt tatgatcaga tcgcggatca tcgccccgac ttcctggtaa
      841 ggtctttgcg cagaggtggc agatttcatg gtgttgtgtc cgttaggtgc ggttaatcaa
      901 attttttccg gatgcggcgt gaacgcctta tccgacctac aacgggcaat tttacggcac
      961 ccgttaagct caaactatac cattagcact ggatgttggc acgtacaggt gggcaaaaaa
     1021 acgaaaactg gtcaaccaaa tattagtttg attgaccagt aagtctgtag ggtaatcagt
     1081 tagttacagc gcagccacac actcgcgcgc accgcgctca cacagctgct gatatgccgc
     1141 cgtcaccgcg ccgacaaagt cggcgttctg cggcagatca tcggcaaaga taccgctcag
     1201 gcccagcagt gctttcacgc ggtctgcacc ctgatactgc gcgttgatct tctggaactc
     1261 cgccagcatc gggtcaacca cgtcaatagc attaccctgc tcatccacgc cctgggtgta
     1321 gcgcatccag ccagccacgc ccagcgccag gtgacgccag ctaccgccgt tttgcaggtg
     1381 cagacgcacc gggtccagca gacgctgcgg taacttctgg ctgccgtcca ttgcaatctg
     1441 ccaggtacgg tgacgcagag acgggttgct gaaacgctcg atcagcagcg tcgcataggc
     1501 gttcaggtcc gtaccttccg gcatcgacag cgttggcgct tgttcctgca tcatcagggc
     1561 aaaggccgct ttgcgataag ccgggttagt catggtgtcg gcaatagttt catagccgcc
     1621 gaggtaaccg aggtacgcca ggaaagagtg gctaccgttc agcatacgca gcttcatcat
     1681 ttcgaacggt acaacatccg caacaaactg cgcgccgact ttatcccagt ccggacgacc
     1741 attaacgaag ttatcttcaa ttacccactg acggaacggt tcgcaggcaa tggcgcacgg
     1801 gtcgtaaaca cccagctggt cagcaatttc ctgtaaggtt tctggcgtcg ccgccggaac
     1861 gatgcggtca accatggtgc acggaaaggt gacgttttct tcaatccatg ccgccagttg
     1921 cgggtcacgc gcctgagcca gcccaagaac ggcaaccttc gccacatgac cgttttcacg
     1981 cacgttgtcg caggacatca ccgtaaatgc tttcagccct ttttcacgac gcagacgcag
     2041 ggcttctacg atgtaaccaa tcgcggactt cggcgcagtc gggttttcga gatcgtgctt
     2101 gatcagcggg ttattgagat ccagctgacc gcttgccgca tcagcgcagt agcctttttc
     2161 cgtgaccgtc agagagacaa tcgccgtctg cggacgcgcc atcgcgttga gaataccttc
     2221 gcaaccgtcg atttccggat gcagcgcttc tttcatcgaa ccgataattt tcagctcggt
     2281 gctttccgcg cctttttccg ccacggtgta cagcagttgc tgttttttca ggttttcgat
     2341 cagcacgcgg tcgttgcctg gcatcaggtt aacttcgcag ataccccagt cgctgtcggt
     2401 aatttccagc agatgatggg tatacagcgc ctggtgcgcg cggtgaaacg ccccgcaacc
     2461 gagatgcaca atgcgtgatt ccagacgaga atgatcccat gacgggcggg caaccggcag
     2521 attgctgtca acaatagtag tcatcgtaaa ctccagaatc cgtcttcaga gagtgacggc
     2581 aaggaagaag gaaccggcac ggcgcgcagc catgccggtg ggtattaacg gctaaagaaa
     2641 gcgcgctgga tcgccagttc gacaccgcga acttcggcca ggcctttcag acgaccaatt
     2701 gcggagtaac caggattggt tttcttcttc aggtcgtcca gcatctgatg accgtggtcc
     2761 ggacgcatcg ggatcaggtc ttctttgcct tccgctttac gacggtgttc ttcttcaaca
     2821 atcgctttca ccacttcgta catatcaacg tcaccgttca ggtgcgccgc ttcgtggaag
     2881 gttttcgggt tatcttcacg catggtggag cgcagatggg tgaagtaaat acgcggaccg
     2941 aactgcttga tcatatcaac cagatcgttg tcagcacgca cgccgtagga accggtgcac
     3001 atggtgaaac cgttcgccat gctgtttacg gtatcaacca tccactgcat atcttcaatg
     3061 gtggaaacaa tgcgcggcag gccgaggatc gggcgcggcg gatcgtccgg gtgaaccgcc
     3121 atacgcacgc cgacttcttc ggcaaccgga ataatcgctt tcaggaagac agcgaagttt
     3181 tcgcgcagtt tggctttgtc gatatctttg tacagctcca ggtgcttacg gaactggtcg
     3241 agggtatacc cttcttccgc acctggcaga ccggcaatga tgttacgggt cagacgcgct
     3301 ttgtcttcgt cgctcatggt ggcgaagcgt tcagctgcct gagcaatttc ttcttcggtg
     3361 taatccgctt ccgcgcctgg acgtttcagg atatgcattt cgaatgcagc gaattcgatc
     3421 tggtcgaaac gcagcgcttt ggagccgtct ggcagcacgt attcgaggtc tgtacgagtc
     3481 cagtcgagca ccggcatgaa gttgtagcac acggtgcgaa tgccgcactg cgccaggttg
     3541 cgcagagtct gctgatagtt agcgatccac tgctcatagt tgccggtgtg ggttttgata
     3601 tcttcgtgaa ttggcacgct ttctacgaca gaccatacca ggcctgcgtc ttcaacgatc
     3661 gccttgcgtt tgaggatctc ttcaacggac catacttcgc cgttcgggat atggtgcagc
     3721 gcggtaacca cgccagttgc gcccgcctga cggacatcag ctaaagaaac cggatcgttt
     3781 gggccgtacc agcgccaggt ctgttccatg atttttcctc tttcacatag tgggttcaac
     3841 agcgaagaga cgcggaaccc cggagttgtt taggtatccg gatgaacaac ccggccacag
     3901 ccgtagcgtc gcatcggatg agatggcgta taagttctac cttttggtaa gacatgtcaa
     3961 cccgaattct aaaattggtt aaccacatca caagaatttc actctcctgg ttgttgctgc
     4021 aaattgcagc aaagggtaat cgatggttat tgatcagagg atgtgacatt catcgcaaca
     4081 atggttgacc aatttacata acatgtcgcc caaaataaca acggttcaac caggtaaggt
     4141 aaaacgttat gcatgtgctt aacattctct gggtggtatt cggcattggc ctgatgctgg
     4201 tactgaattt gaagttcaaa atcaattcga tggtggcttt gttggtggcg gcgctgtccg
     4261 tcgggatgct ggccggtatg gatttgatgt cgctgctgca caccatgaaa gcaggtttcg
     4321 gcaacacgct gggggaactg gcgatcatcg tggtgtttgg tgcggtcatc ggtaaattga
     4381 tggtcgactc cggcgctgct caccagatag cgcataccct gctggcacgt ctcggcctgc
     4441 gctatgttca gatatcagtg attatcatcg gacttatttt tggtctggcg atgttctatg
     4501 aagtggcctt tattatgctg gcgccgctgg tgatcgtaat tgccgccgaa gccaaaatac
     4561 cgttcctgaa actggctatt ccggcagtag cggcggccac caccgcacat tcactgttcc
     4621 ctccgcaacc gggtccggtg gcgctggtga atgcttacgg cgcagatatg gggatggttt
     4681 atatctatgg cgtcctggtg actattccca gcgtaatctg tgcgggactg atcttgccga
     4741 aattcctcgg caaccttgag cgcccgacac cgtcatttct gaaagcggat caaccggttg
     4801 atatgaacaa cctgccctct tttggtgtct cgattctggt gccgctgatc ccggcgatca
     4861 ttatgatctc caccaccatc gccaatatct ggctggtaaa agatacccct gcctgggaag
     4921 tggttaactt tatcggttcc tcgccgattg caatgtttat tgcgatggtg gttgcattcg
     4981 tactctttgg taccgcgcgt ggtcatgaca tgcagtgggt gatgaacgct tttgaaagcg
     5041 cggtgaagag cattgcaatg gtgattctga tcatcggtgc gggtggcgtg ctgaagcaga
     5101 ccatcatcga caccggcatc ggcgacacca tcggcatgtt gatgtcccac ggtaatatct
     5161 cgccctacat catggcatgg ctgatcaccg tactgattcg tctggcgacg ggacagggtg
     5221 tggtttcagc gatgaccgcc gccgggatta tcagtgctgc aatccttgat ccagcaaccg
     5281 gtcagctggt tggcgtgaat ccggcgctgc tggtactggc gacggctgcg ggttccaaca
     5341 ccctcaccca cattaatgat gcctcattct ggctgttcaa aggttacttt gacctgtcgg
     5401 taaaagacac gttgaaaacc tggggactgc tggagctggt caactccgtg gttgggctga
     5461 ttattgtgct gattattagc atggtagcgt aaaagaacac cagatcctgc cattggcagg
     5521 ctctgactga tgacaaagtc taaaaatgtg ccctcaccct aaccctcgcc cctccgggga
     5581 gagggttagg gtgaggggaa cagaccagca aagtgcgctt tgtttatgcc agatgcgacg
     5641 ctgacgcgtc ttatctggcc tacaaagggc taacgtgcag gttttagctt caggtaatat
     5701 tgcatacctg cattagcaat gccctgtgat ttctttattg ataaacaaaa gtcacgccaa
     5761 taatcgattg cacattccct gcagtcacct gccctccggt acgtgcgtaa tttgccgtta
     5821 atcccagact taccgccgaa gtccctactg ctcctaacga taccgtgtta ttcgctggaa
     5881 taatcgtacc gttgcgcgtc aactgtacgc cgacgccctg cgcgggtgaa aacgacgcgg
     5941 tattggtgaa aatcgagttg cccgcatctg cggttgtgcc ggagaggtaa taccccaggt
     6001 tttggctttt cgcacaataa acggtaagag gaatcggcac tgaaccaggg tagtccggca
     6061 aagtaacggt gacatcacga gcagaaacat cacagccgcc agtgggcacc accacatcat
     6121 tattggcgta aatattccac acaaactgga aatcatcgct gttatagttg ttggtctgtc
     6181 gcaaaataag cacggcaatt aatgagcctg ctttaatcgc cactcccccc gcactgctca
     6241 caggcgtcaa ataaagcgcc accggccacg gcttatccgt tctcgaatta taaacaaccc
     6301 gcggcgtttc gctggtagta gggaaaggat agctactgcc attatatttt acggtcccgg
     6361 aaaaactaga taacacgccg ccataagccg aacctcgttg cagtgtgaca tagtctgtaa
     6421 tggtttccgg gtaatcgtta tggcaaaaga tttgcgtcga aagatccacg accaggtttt
     6481 gccccacatt cacggcaggc gcaaggttta cataaacatt ggcgctgcca ccgccaatag
     6541 ggatagcggt accattggcg gttttacagg cgaatgacca ggcatttacc gaccagccca
     6601 tcagcagtac agcaaacagg gtaataactc gtttcattac aatcatctct tcgggttcag
     6661 ctgtaggtat aggtgatgct aatcactgcc tgaatggttc cctgagtggc tccgccattt
     6721 accgtcaatg ctctgacctg taacgggaag tgcgctgata gtgaggaatc atccacctga
     6781 actgttttgg ttgcgccagt attcaatgtg ttgccactgt catcctgtag ctctaactgg
     6841 atgttttgcg cggtcccctg gtttttataa tatccggtac tgtcggctgc cccgctgaag
     6901 ctggcagtga ccctcgacgt tcccaccgga caattagtca actcaagcgc aacatcatgc
     6961 caggccgatg ccgccccggc agacataaga ctgaaagaat aaagatcgcc gagatctacc
     7021 gtggcattgg tggtggaaac tgtgcacggt ttggcgacga ccttaccgtt caccgtgatg
     7081 gtgacatcgg ctgcctgtat cgtcgcactt gcgaacgcca acattgccgc caataaatac
     7141 ccacgtttgc accatttcat gagcatctcc agttactgat attcaagagt gaaggtagcc
     7201 gtggcattaa tatgccccgc agtgacaggc acctgtgtcg ccattagccg ggcgtaaaaa
     7261 ttcaacgtat ttggtttacc cggcgtcagg gtcgtccacg aaatcgcgga cgatggggca
     7321 ttaaggggta tctcattttg ctgctcattc agaagctgta ttcccagtcc cgcagccgct
     7381 gacaccgtat tttcaagtgc aagcaggttg gcattgtggc tatctgcaac gccggtaaac
     7441 ccaaccttta cggcagaaac ggcgttacca cagggtgaca gcaaaatacg aaatggaaca
     7501 acaggagtcg tcgcgccaat gttgttaaat tgcttcgccg cgttttccat cagatcaaca
     7561 gtaaaattgg ttgattcagc ggccacacta cagccattat ctctgacata gccgcggata
     7621 gtaatcgtgc tatccgcagc caaagcgtga cttaccgcca gccacaaaaa agcgcaaaga
     7681 agataaaaag gtttgtttct catcacgccc ccttaacgac attcagctga tagctgggtt
     7741 aataactgct gctgactctc tggtggcagt tgataattgg cgacacagtg agcattttcc
     7801 tcttctcccc atttcacctg cacttttccc gctaaaggca ttccgctgag gtaaacctga
     7861 ccattatccg caacaatgcc gctactctgg ctactctctg atgtcaccat cgccccaaac
     7921 ggcagcggct tattattgtg ggttagcgtc atgagcagtt ttatcccaac gcgcgcttta
     7981 aactctgctc gcacgatcgc cccacgggtg ggaacaacgt tagcgaccgc attatctaaa
     8041 tcgacgttat cagccagggt attggtatcc agcgccactc tgttttcccg atattcagtg
     8101 gcataaggca gcacggcata accccgccag tcggtacgca ctcctgtctg gttttcgact
     8161 tttgcatctt ttgcgccagg cgctttaaca agcaccaccg tatcgtttaa cggctgcccc
     8221 agcgttacgc cattggcatg agccagtacc ccaccgctga ctccgtaata gagctgctta
     8281 atatcatcgc tatggctgta accgatattg gcattgccgt aaccaccgcg ataattcagc
     8341 gtggcgtagc ctgtacttcc gctatttcca tcgcctcccc cggcataacc ggtttgcacg
     8401 ctatagctga ggttgttgtc ttccagcaac gtaccgtata caccggccag attggtcatc
     8461 cgaccgttga gatcgtgtga catgctgtag ctggcactgg catgtcgcca ctgagattta
     8521 ctgtcagaac gcagccaatg gctgaaaggt atattgacgt taagagctaa catctgatcc
     8581 cgtccttttt gccaggcgtt tttcgtcagg ctatagctga gcgtccagtt aatatcttcg
     8641 aacgcagtat ttaatccagc ctggaattgc tcatcgacat tactcgttcc ccaataagtt
     8701 tgatggctac cactcaaata cagtgttgat gtgcgcccga gttgctgagt aacggtgagt
     8761 tgtaatttcc cgcgtttgtt ataagcgaga ttgtaatagt cggtgaattt cggcttaacc
     8821 tgaataactc catcctgtgt ttcgatgttg tagccattca ttcgactgta tgttgtatca
     8881 gcgaaattaa aatacccact ggtcgaataa cggtaaccca ctaactgaat attcgtacct
     8941 gactcattga gcgatttgtt ataaagaaaa cgcaccgatt gtccgtcatg ctgactgtca
     9001 tcgggaagtg tggaattagc ctgcgtcata tccacagaca gagcgcccag tgcccccata
     9061 tttttcccga taccgaaatt aaacgcacga taacgatccg ccagttgcgt tccaccatat
     9121 attgtccagc cagccggaag gccgtggagt aatgtactct ggaaaaagcg gggtttttcc
     9181 tgttgtgcat ttccactacg gtattctcct gccgtaatgg aataacgtgt atgcccttca
     9241 cgctgcaaaa gcgggactga cgaatagggt acggtaaaaa tctgcgtact gccgtcagcc
     9301 tctttaatcg ttacctgcaa gtcaccacta ttacctgcgg catagatatc gttgatggta
     9361 aaaggccccg gtggcaccgt actattataa atgtcatacc cattttgttt aatagtgacc
     9421 tgtgcagtac cacgagcaat accgtggatc accggggcaa atcctctttg gctatcgggt
     9481 aacatattgt catctgaggc caattgtgcg ccgcgaaagt taataccatc gaaaatatcg
     9541 ccctgagtat aaccatcacc cagcgtcagc cgggaacgta acggaattat gtctcgctca
     9601 agccaggtat tgatatgctg ccatttattt ttgctacctg atgaactgtc gctactgtta
     9661 taactccagg tggtattgtc gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg
     9721 tttaaatatg cataatggct gttaccccca attcgattct gtacactatt tccgctgaaa
     9781 ttataattga gcaatccggc attaataccg ggatcccata actcaggagg aatataacca
     9841 cgcgcgcgat tactcataaa tgcctgaggg atcgtcaggt tgagtcgctg ctgacccaca
     9901 tccagttgcg cagtagcgtc atggatcatt gaggttaatg gcacgcaggc atcatccgcc
     9961 agcagattca taccggagac agaagccgta ttcagcccca tactggcaag ttgcgcgcgt
    10021 gtcaggcagg gaacaatccc ttgttcgctg tcgcccgtat taaatgtgac atcacgcgtt
    10081 gccatataac cattattcaa atagatatca acacgatacg tccctggcgg taattcttgc
    10141 ccattttcaa aacgcgataa atcagccaca gcctggggat catccgctaa aaaacgcggg
    10201 ttaaaataga gttcggcaga tgacaaaggt gcctgtgcgg caaaagcac
//
LOCUS       DADSZD010000155        10189 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.156, whole genome shotgun sequence.
ACCESSION   DADSZD010000155 DADSZD010000000
VERSION     DADSZD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.156"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(172..1446)
                     /locus_tag="J1Z28_003585"
     CDS             complement(172..1446)
                     /locus_tag="J1Z28_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA7718386.1"
                     /translation="MAINVLSPKQIENAKPKTNDYKLTDGGSLYLLVRKTGGKYWRMN
                     YRFDGKQVTLALGVYPDVSLATARKRRDEARQLLADGRDPREAKKPKKEDPISPTFES
                     VAREWHSGTMGHPEWKEITRKKILREMENHLFPAIGSKPIDTLKTRDLMPMLVEMTEK
                     GIGATTSRVKTTMTSIFRYAVQRGIVDYNPAHDLKGAITTPKVRHRPALPLERLPELL
                     AKTRSYTGRPLTRLAVLFSLHTFVRSSELRHARWEEIDIENGLWTIPGQREEIAGVKF
                     SERGAKMGAAHYVPLSSQAIGVLEDIRKISGEYLLVFPGDSNPYKPMSENTVNKALRT
                     MGYDTQSDVCLHGFRAMACSALTESGLWSRDAVERQMSHQERNEVRAAYVHLAQHMQE
                     RRRMMQWWSNYLELNKDEFHAPYDLSGINIIN"
     gene            complement(1633..1704)
                     /locus_tag="J1Z28_003586"
     tRNA            complement(1633..1704)
                     /locus_tag="J1Z28_003586"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1669..1671),aa:Arg,seq:cct)
     gene            complement(1780..2712)
                     /locus_tag="J1Z28_003587"
     CDS             complement(1780..2712)
                     /locus_tag="J1Z28_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBA7718387.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGIVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            3006..3761
                     /gene="mlaA"
                     /locus_tag="J1Z28_003588"
     CDS             3006..3761
                     /gene="mlaA"
                     /locus_tag="J1Z28_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBA7718388.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(3823..5163)
                     /gene="fadL"
                     /locus_tag="J1Z28_003589"
     CDS             complement(3823..5163)
                     /gene="fadL"
                     /locus_tag="J1Z28_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBA7718389.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTQQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            5535..5819
                     /locus_tag="J1Z28_003590"
     CDS             5535..5819
                     /locus_tag="J1Z28_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 family protein"
                     /protein_id="HBA7718390.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5992..7302
                     /gene="fadI"
                     /locus_tag="J1Z28_003591"
     CDS             5992..7302
                     /gene="fadI"
                     /locus_tag="J1Z28_003591"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBA7718391.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     GDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7302..9446
                     /gene="fadJ"
                     /locus_tag="J1Z28_003592"
     CDS             7302..9446
                     /gene="fadJ"
                     /locus_tag="J1Z28_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBA7718392.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPTTERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRNIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPRGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCATHTIFASNTSSLPISDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            9649..10134
                     /gene="sixA"
                     /locus_tag="J1Z28_003593"
     CDS             9649..10134
                     /gene="sixA"
                     /locus_tag="J1Z28_003593"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBA7718393.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2468 a         2446 c         2617 g         2658 t
ORIGIN      
        1 gtataggtgt aataaatcat caatctcgca ttctaatagt cttgaagcgc gttcaaatga
       61 acaatattct aatggtggta tttttttgtc aatccataga tttcctaaga atatagtttc
      121 attctctttt gaatgctgca ttatgacctc tgttttaaat cataagaaaa tttaattgat
      181 aatgttaata cctgataagt cataaggagc atgaaattcg tccttattta gttccagata
      241 attagaccac cattgcatca ttctgcggcg ttcctgcatg tgctgcgcaa gatgcacata
      301 tgctgcccgc acttcgtttc tttcttggtg actcatctgc cgttcaactg catcgcgtga
      361 ccagagtcct gattcggtca gtgcagagca ggccatcgcc ctgaacccat gcaagcaaac
      421 atcactttga gtgtcgtaac ccatcgttcg cagtgcttta ttaactgtgt tttcgctcat
      481 cggtttatat gggttgctgt caccggggaa aaccagcaga tattcaccac tgattttcct
      541 tatatcctcc agtaccccga tagcctgact agatagcggg acataatgag cagcccccat
      601 ctttgcaccc cgctctgaga atttaacccc tgcgatctct tcacgttgtc ctggtatcgt
      661 ccatagccca ttttcaatgt cgatctcctc ccatctggca tgtcgtagtt cgctggatcg
      721 cacaaaagtg tgcagggaaa atagaactgc taggcgagtc aatggtcttc ccgtgtaact
      781 tctcgttttc gctaacagtt caggtaaacg ttcgagaggt agtgcggggc gatgtcgtac
      841 tttgggggtc gtaattgcac cttttagatc gtgggcaggg ttgtaatcga caattccacg
      901 ttgcacggca taacggaaga tgctcgtcat agtggttttt acccggctcg ttgtggcacc
      961 aattcctttc tcagtcatct caaccagcat cggcatcaag tcacgggttt tgagcgtatc
     1021 gataggcttt gaaccgatgg caggaaagag gtggttttcc atttctcgga gaattttttt
     1081 acgggtgatc tctttccact caggatgccc catcgttcca ctatgccact ctctggcaac
     1141 actctcgaac gtgggggaaa ttgggtcttc ctttttgggt ttctttgctt cgcgaggatc
     1201 acgaccatcg gctagtagtt gcctggcttc atcacgccgt ttccgcgctg ttgcaagtga
     1261 gacatctggg tagaccccta atgccaatgt cacttgtttt ccatcaaaac gataattcat
     1321 acgccagtat tttccaccag tctttcgtac cagtagatac aaactccctc catcggtaag
     1381 tttgtaatca ttggtcttcg gcttggcgtt ttctatctgt ttgggggaaa gcacattaat
     1441 ggccatatct gtactcattt tggtaaattg atggtatctg catatcgaac tggggaatac
     1501 catcacttat accatcacga ggttgtaatg tcatgaaatt aactgaaatg aaaatcaggc
     1561 cgattcggtg ataacttact gttttttaag tggttatgta atgaagtgaa atgtagagaa
     1621 aggtctttaa agtgtcccct gcaggaatcg aacctgcaat tagcccttag gaggggctcg
     1681 ttatatccat ttaactaaga ggacaatgcg gcatgagtat acccgctaat ggactacggg
     1741 gtaagtacac agccgctcga ttgcttaaac cctcgccatt tatgccgggt ttttatcatt
     1801 tttcttaatg ttttccgcac gttctgcttt ttgacgtgct tctgctttgc gcttgttgct
     1861 catgtcgtta cgaatctgtg catgactcat taacgcaaag ataaaggtgc cgccgcagat
     1921 gttccccgct aaagtaggta gtgcaaaggg ccagatgaaa tcgctccagt gcagcgtgcc
     1981 gttaaacacc agatagagga tttcaacaga accgacaacg atgtgggtgg tgtcacccag
     2041 ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacccg ctgcaggaaa
     2101 catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa acatctcgct
     2161 gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag tttcttcatt
     2221 gaagataggc atatattcaa atgcccacgc agcaatacct gtcccgagaa tattacccag
     2281 cagcacgatg ccccataacc gcataagtaa gccgacgttg ctcattgtcg gtttttgcat
     2341 gacgggtagt accgcagtta cggtgttttc ggtaaataat tgctggcggg ccattatgac
     2401 gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg gcacgccttc
     2461 cagttcgaca tgaaatatcc cttttgccag tagcgaagcg cccatcgaca ggcccgccgc
     2521 aatggctgac cacagtagcg ccattgcgtc gcgttccagc tctttttcac catcctggcg
     2581 gatatgctca tgaattgcca tcgcccggga ggggagtcgg tcttcatcta tttctatttt
     2641 tttaccgcgc tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt
     2701 gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt agcggctttt
     2761 ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggccgcgtt tgcctttttc
     2821 tgttgctatt gaatcaagta gcctacaggg cggcgattac caggctatga tcaaatcagc
     2881 aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac ctaaacaggc
     2941 ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa aacagggaga
     3001 catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg
     3061 cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttt aaccgcacta
     3121 tgtacaactt caacttcaat gtattagacc cgtatattgt tcgaccggtc gctgtcgcct
     3181 ggcgtgatta tgttccgcaa cctgcgcgta acggtttgag caactttacc ggcaaccttg
     3241 aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag gggatggtcc
     3301 actttacccg ctttttcctg aataccattt tgggaatggg cggctttatt gatgttgcag
     3361 ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt acgcttggtc
     3421 attatggtgt gggttatggg ccgtacgttc aattaccgtt ctacggtagc ttcacgctgc
     3481 gtgatgacgg tggtgatatg gcggatagtc tttacccggt tctttcctgg ctgacctggc
     3541 cgatgtctgt gggtaaatgg acgcttgaag ggatagaaac tcgtgcgcag ttgttggatt
     3601 ccgacggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgtgaa gcgtacttcc
     3661 agcgtcatga tttcatcgct aatggcggtg aactcaaacc gcaggaaaat ccgaacgcac
     3721 aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaac aaaaaaggtg
     3781 agtcgcaaaa ctcacctttt ttcttattaa gctaacgcgt tatcagaacg cgtagttaaa
     3841 gttagtaccg aacagccagg ctttaccttc agactcgaac tgatatgggc cttcgttaat
     3901 tttcacgctc tgaccgtgca tataagaaac accaacgtcg actgaagcat ctttattaaa
     3961 tgcgtaagtc gtacctgcac tcagccagaa acggtcctgg tccggaatgg agatagaacg
     4021 attctgtgcc ggaactgggc tgtcatcaaa ggcgataccg gtacggaagg tccagttatc
     4081 atcgtagtaa taagtggtac ccaacgcgat gcggtaagca tctttaaagc cttcatgttt
     4141 ctggaacagc gtgtcgccac tcgttgaggt cgctttcagc tgctggaact gactccagct
     4201 ggtgtaagcc aggctatagt gaattgccca ctgcggatca acacggttat aacccgaaac
     4261 ctcccacatt tcaggcaggt tcagcgtcag ataacctgac tgcgttgcgc cacctgtcgc
     4321 ggtaggaatt ggcaaaccgt agttattaaa tgcacgatta agatcgctgc tgtagttacc
     4381 tttgaagtca attttcactt cagaacggta ggtcagtgca tagcggttat ttttatccag
     4441 ttcatacagg attccggcgt tccagccaaa accccactgg ttaccgttca gatgagcgat
     4501 tttggtatta ctgtcgatac cgttggcggt agctgccaat gcttgccctt gctgagtttg
     4561 tccagcagga gattgcataa tctgaccggc aaccagctgc cccagatcgc ctgcgaaacg
     4621 ttcaattttc gcgcgagcgt agacggcgtt gaaaccaaga ccaaagctcc atgcattatt
     4681 taagcgatac gcaccgctta agttcaggtt catggtttca aggtcggttg taccaccgac
     4741 agaaccgcct gcataagtat cgttaaactc tgttgccaga ccatagttag aggtaataga
     4801 agcgccccaa ccaaattggt cgttaatcgg tgcaacaaag tgcatgttcg gaacccatgc
     4861 cgtaggcgcg atgttatcgg ctttcaggct acgaccagac ggagacgttc cgctgatatt
     4921 tacatccgga tcaatataaa ccgcacccgc agaaaatgtc gggcggtcaa acatggtaat
     4981 caatgcgggg ttacggctaa cgttacctgc atcatcggca attgcacctt cccctgaata
     5041 agcccggccc aggccagagg aagaaaattc gtttaactga aagcctgccg accaggcctg
     5101 ggtggagata agtgccactg cgactgcgag agcagacttt gtaaacaggg ttttctggct
     5161 catgaccata acctcaatga tttattttta tacaaactat gttacgtgct gtaacaggag
     5221 cgcgaagtgt agggtctgag gtacatctta gaaatcagac cagtggcgag agtataggtc
     5281 ggaccagctg gaatgttgca agtatgtttc tatctttttt cagatatgat ctaagaaacg
     5341 cgatccagct ggcaaaattg ggaggtggcg cactcatgga ttcgggtgaa ttttgtttta
     5401 gatcattttt aagtgtgatt tcggtcactt atccgatttt gcagaattaa caactggagc
     5461 agcactttcc ggaagcgtaa aatatccaca acgtttatct ggcaccttgg gtgcacatac
     5521 agaggaaatc aacgatgagt aaatgtagtg ctgatgaaac cccggtttgc tgctgtatgg
     5581 atgttggcac cattatggac aactccgact gcaccgcgtc ttacagccgt gtgtttgcaa
     5641 accgcgcaga agcagaacaa acgctggcgg cgctgactga aaaagcccgt agcgtggaat
     5701 ccgaaccgtg caaaatcacc ccgactttca ctgaagagtc tgacggtgtg cgcctggata
     5761 tcgatttcac ttttgcctgc gaagctgaaa tgctgatctt ccagctgggt ctgcgttaat
     5821 tcctctcggg tacatacgcc cctgtcagtt gctggcaggg gcgttttctt ttctcttctt
     5881 cttttctctc tgtgtcatag ctcccacttt cccttttctc cacttggcta aatgtaaaaa
     5941 aatggttaag actatgatca ggtcagacca ctttatttac aggggagtgt tatgggtcag
     6001 gttttaccgc tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6061 ccttttgccc gacaggcgac ggcttttcat ggcattcccg cggttgattt agggaagatg
     6121 gtggtaggcg aactgctggc acgcaccgag atccccgctg aagtgattga acaactggtc
     6181 ttcggtcagg tcgttcagat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6241 actggaatga atgtgcatac cgatgcttac agcgttagcc gcgcttgcgc taccagtttc
     6301 caggcggttg caaacgtcgc agaaagcctg atggcgggaa ctattcgagc ggggattgcc
     6361 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgcgcgta
     6421 ctggttgatg tcaacaaagc ccgcacaatg agccagcgcc tgaaactttt ttctcgctta
     6481 cgtttgcgtg acttaatgcc ggtgcctccg gcagtggcgg aatactccac tggattacgg
     6541 atgggggaca ccgcagagca aatggcgaaa acctacggca tcacccgaga acagcaagat
     6601 gcactggcgc accgttcgca tcagcgtgcc gctcaggcgt ggtcagaagg taaactcaaa
     6661 gaagaggtga tgactgcctt tatcccccct tataaacaac cgcttgtcga agacaacaat
     6721 attcgcggta attcctctct tgctgattac gcaaagctgc gcccggcgtt tgatcgtaaa
     6781 cacgggacgg taacggcggc gaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     6841 ctgatgactg aatcccgggc gaaagaatta gggctggtac cgctggggta tctgcgcagc
     6901 tacgcattta ctgcgataga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     6961 ccgctggcgc tggaacgtgc cggattgacg atgggcgatc tgacattgat cgatatgcac
     7021 gaagcctttg ccgcccagac gctggccaat attcagttgc tgggtagcga acgttttgct
     7081 cgcgacgtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7141 cttggcggtt cgattgctta tggacatccc ttcgcagcga ccggcgcgcg gatgattacc
     7201 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagtgac cgcctgtgct
     7261 gcgggtgggc ttggcgcagc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7321 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccgggtgaga
     7381 aaatgaatac cctgaaggcg gaatttgcct cgcaggtacg cgccattatt aagcaactcc
     7441 gtgaaaacaa agagttgcgt ggcgtggtgt ttgtctccgc gaaaccagac aacttcattg
     7501 ctggcgcaga catcaacatg atcggcaact gcaaaacggc gcaagaagcg gaagtactgg
     7561 cgcggcaggg gcaacagtta atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     7621 ctattcatgg cgcttgcctg ggcggcgggc tggagttggc gctggcgtgc cacggtcgtg
     7681 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     7741 ccggttcagg cggtacccag cgtttaccgc gcctgatagg tgtcagcaca gcattagaga
     7801 tgatcctcac cggaaaacaa cttcgggcga aacaggcggt aaagctgggg ttggtggatg
     7861 acgtcgttcc gcactccata ttgctggaag ccgctgttga gctggcaaag caggatcgcc
     7921 catcttcacg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     7981 tgctgttcaa aatggtcggt aagaaaacag aacacaaaac gcaaggtaac tatccgacga
     8041 cagaacgcat cctggaggtt gtcgaaacgg gattagcgca gggcaccagc agcggctatg
     8101 acgctgaagc acgggcgttt ggcgaactgg cgatgacgcc gcaatcgcag gcgctgcgca
     8161 atatcttttt tgccagtacg gaagtgaaaa aagatcccgg cagtgatgcg ccgcctgcgc
     8221 cattaaacag cgtggggatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8281 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcgg ggcataaatc
     8341 atgcgctgaa gtacagttgg gatcagcttg agggcaaagt tcgccgtcgt catctcaaag
     8401 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat tgcggctttg
     8461 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     8521 tggcggaagt ggagcaaaat tgcgctactc ataccatctt tgcttcgaac acgtcatctt
     8581 taccaattag cgatatcgcc gcgcacgcca cgcgaccaga acaggttatc ggcctgcatt
     8641 tcttcagtcc ggtcgaaaaa atgccgctgg tagaaattat tcctcatgcc ggaacatcgg
     8701 cgcaaaccat cgccaccacg gtaaaactgg cgaaaaaaca aggcaaaacg cctattgtcg
     8761 tgcgtgacaa agctgggttt tacgtcaatc gtattctggc gccttacatt aacgaagcta
     8821 tccgcatgtt gaccgaaggc gagcgtattg agcatatcga tgccgcgctg gtgaaatttg
     8881 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     8941 ttatgcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9001 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg ccggggtttc tatctttatg
     9061 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcccgc catttacccg ctcattggcg
     9121 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9181 tgaatgaagc agtacgttgt ctggacgagc aggttatccg tagcgtgcgt gacggggata
     9241 ttggcgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9301 attctctcgg cgcaggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9361 cccgttttac cccttgcgat cgtttagttg agatgagcga gcgtggggaa agtttttgga
     9421 aaacaactgc aactgacctg caataagaag gtcaaagtta tatgaatccg cgctgaatgg
     9481 cggagttttg ctcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
     9541 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
     9601 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
     9661 atcatgcgtc acggcgacgc agccctcgat gccgccagtg attcggttcg tcctctgacc
     9721 actaacggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
     9781 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
     9841 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
     9901 gttggtctgg tcggtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
     9961 gtgatctccc acttaccgtt agttgggtat ctggttgccg agttatgtcc gggcgaaacg
    10021 ccgcctatgt ttaccacttc agcgattgcc agcgtaacgc ttgatgaaag cggaaaaggc
    10081 caattcaact ggcaaatgag cccttgtaat ttgaagatgg caaaagcaat ttaagcaatc
    10141 aatgcccggt tacgtgcgcg tacaccgggc agtttctccg tcatacaat
//
LOCUS       DADSZD010000156        10144 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.157, whole genome shotgun sequence.
ACCESSION   DADSZD010000156 DADSZD010000000
VERSION     DADSZD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.157"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..4305
                     /locus_tag="J1Z28_003594"
     CDS             <1..4305
                     /locus_tag="J1Z28_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7718394.1"
                     /translation="DGQGSTGTEIAGNNAVVNQDGLLDVSGGGHGIDITGDSATVINK
                     GNITVTDKDSVGVLINGDRATFANTGHIDVNNSATGMSITTSEGAISQAGSMNVGDFS
                     TGMALSGNNNSVTLAAKDLNVTGQKATGVNISGDNNAVDIAGNILVDKDQTADNAADY
                     FFESSVGVNVTGNNNTVSLNGELTVVSDSELTSRSHGKRDGSQENISGLIVSGDNNTI
                     SLNGGIQFIGEQKILADGSDIASLRKGFSDTSIISVDGNSSVYLNGTSTIGGDLPLGY
                     TSIIQLKNKAILEIGRDALLGVKDIKSFNNYYEPVPKIIKATTGSKVINNGNIDIQNI
                     GFISVSGEDTSGTNNGNITLSQYDYGNMMSGTNALLSTDGGSVVNNGTIIGKVMEQSS
                     VINRFETTDVTYDELFNNSVTGITGMLSKTGAMGINNVNGIIDMYGRGSVGMLAVTQA
                     IIENAGTIKLDSLWVDANDTTALPDNIAISNARYYGAGMAVGSDSYGSPDANVTAINQ
                     LGGVISVYNAGVGMAAYGGSNMVINQGTINLEKNENYSSSLSANTLVGMAVYQHGTAI
                     NDKTGVININVDTGQAFYNDGTGTILNYGEINLLGSPMDSADPHMGATPDNLDLLSAL
                     TGSGETDMRTASSGGFVTTKALANYGNETLNSNVTAKAWLYNQDKANLTINGELSVGQ
                     GLENSGLLDSDTISAAANVYNRASGSIITDLLMLTSGNSFFNQGNFSGSIVGNSYRQN
                     VVNTGSMTVTADGTSLIGGSFVLYNEAGAILSNSNSAVSGGENAIVNITRTSDSLAQV
                     NRGTITATNGYSAIKTASTGSNSNGKWIWNTDTGVISGVNPNAPLIDLGRGYNFANAG
                     TINVQGDGVVAISGGTTSYTVQLVNSGTINVGTAQGKADGTNGTGLIGIKGNGRETTI
                     NNAQSGVINVYADNSWAFGGQTKTIINNGEINLLCDMGCDIYAPGTTGTLNDHNSTTD
                     IIVPAATSTPTQGSVPTVPADSSAQQKLTNYTIGTNSDGTSGMLKANNLVISDNVKVN
                     TGFSAGTADTTVVINDVFKGENISGAENISSSTVMWNAQGSTDASGNVDVTMTKNAYT
                     DVVTDSSVNNVAQVLDTGYTNNDLYTSLNVGTTAELNSALKQISGSQATTVFNEARVL
                     SNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNDTQYDMMALRKSLTLTEHQNLSLE
                     YGIARLEGNGSDTAGDNGVTGGYSQFFGLKHQMAFDNGMSWNNALRYDVHNLDSSRSI
                     AYGDVNKTADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLEGGYQERNAG
                     DFNLSMNSGSETAVDSIVGLKLDYAGKEGWSANATLEGGPNLSYVKSQRTASISGAGS
                     QRFNIDDGQNGGGFNSLATMGVKYSSQESALQVDAFHWKEDGISDKGVMLNFKKTF"
     gene            4525..5391
                     /gene="pdxI"
                     /locus_tag="J1Z28_003595"
     CDS             4525..5391
                     /gene="pdxI"
                     /locus_tag="J1Z28_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HBA7718395.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVATSLNATPMQVALAWLLQRSPNILLIPGTSSVGHLRENMASDELVLTDDV
                     LATLDSISQKAG"
     gene            complement(5432..6037)
                     /gene="azoR"
                     /locus_tag="J1Z28_003596"
     CDS             complement(5432..6037)
                     /gene="azoR"
                     /locus_tag="J1Z28_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBA7718396.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            6238..10140
                     /gene="hrpA"
                     /locus_tag="J1Z28_003597"
     CDS             6238..10140
                     /gene="hrpA"
                     /locus_tag="J1Z28_003597"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBA7718397.1"
                     /translation="MTEQQKLTFTALQQRLDSMMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDHLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         2671 a         2340 c         2750 g         2383 t
ORIGIN      
        1 gacggacagg gttcgaccgg aactgaaatc gcgggtaaca acgcggtggt gaaccaggac
       61 ggcttactgg atgtgagcgg cggcggtcac ggcattgata tcactggtga tagcgccact
      121 gtaatcaaca aaggcaatat aactgtcacg gataaagatt cggttggtgt gctgattaac
      181 ggtgatcgag cgacctttgc caatacgggt catattgacg tcaataattc agctactggc
      241 atgagcatca cgaccagcga gggggccatc tcccaggcag gtagcatgaa tgttggtgac
      301 ttttcaacag gcatggcgct cagtggtaat aacaacagtg tgacgttggc ggcgaaagat
      361 ctgaacgtga ccgggcagaa ggcaacgggc gtcaacatct ctggtgacaa taatgccgtt
      421 gatatcgctg gcaatatctt ggttgataaa gatcaaactg cagataatgc ggcagactat
      481 ttcttcgagt catctgtcgg ggtgaatgtc acgggtaaca ataatacagt atctctaaat
      541 ggcgaattaa cagttgtctc tgacagcgaa ttaacctcaa gatctcatgg caaacgtgat
      601 gggagtcaag aaaatatctc cgggctgatt gtgtctggtg ataataatac aataagccta
      661 aatggcggta tacagtttat cggtgagcaa aaaatactgg cggatggttc agatattgca
      721 tctctgcgta aaggatttag tgatacctcc ataatcagcg ttgatgggaa ctcatcagta
      781 tatttgaatg ggacttcgac aataggtggc gaccttcctt taggctatac aagcattatt
      841 cagttaaaaa ataaagctat cctggaaatt ggtcgtgatg ctttgttggg tgtaaaggat
      901 attaaatcgt ttaataacta ttacgagcca gtaccgaaga ttataaaggc tactacaggt
      961 tctaaggtca tcaataatgg caatatagat atccaaaata tcggttttat ttcagtgtca
     1021 ggtgaagata cgtcgggcac taataatggc aacattacgc tatcacagta cgattacggc
     1081 aacatgatgt caggtactaa tgctctgtta agtaccgatg gaggcagtgt tgttaacaac
     1141 ggcaccatca tcgggaaggt catggagcaa agtagcgtta ttaatcgatt tgagacgacc
     1201 gacgttactt atgatgagtt gtttaataat agcgttacgg gcataaccgg aatgctttct
     1261 aaaaccggtg ctatgggcat taataatgta aacggcatta ttgatatgta tggacgcggt
     1321 agcgtgggta tgttggcggt tactcaagcg attatagaaa atgctggcac catcaaactg
     1381 gactctctat gggttgatgc taacgatacc actgcgttac ccgataatat cgctatcagt
     1441 aatgcccgtt attatggtgc agggatggcg gtaggttcag atagctatgg tagcccggat
     1501 gcaaatgtca cagctattaa ccaattaggt ggtgtgatta gtgtctataa tgctggggtc
     1561 ggtatggccg cttacggcgg tagtaatatg gttattaacc aaggtacgat taacctggag
     1621 aaaaatgaaa actatagcag tagtctgagt gcaaatacgc tagttggtat ggcggtttat
     1681 caacacggca ctgctattaa cgataaaact ggcgtgatta atattaatgt cgataccggc
     1741 caggcgtttt ataacgacgg tacagggaca atcctgaatt atggtgagat aaaccttctc
     1801 ggttctccaa tggacagtgc agatccccat atgggggcta ccccagataa tctggatctg
     1861 ctgtcagcac taacaggcag cggcgaaacg gatatgcgaa ctgcttcaag tggtgggttt
     1921 gtgacgacca aagcgctggc aaactacggc aatgaaacgc taaatagtaa tgtgaccgcc
     1981 aaagcgtggc tttataacca ggataaggcc aatctgacaa ttaacggaga gctatccgtt
     2041 gggcaagggc tggaaaatag cggattactg gattctgaca ccattagtgc agcagcgaat
     2101 gtctataacc gtgcatcggg tagcattatt accgacctgt taatgttaac gagtggtaac
     2161 tcgtttttta accagggtaa tttcagcgga tctatagttg gcaacagcta taggcaaaat
     2221 gtggttaata ccggaagtat gacagtcacg gctgatggta catcgctcat cggcggcagc
     2281 tttgtgcttt ataacgaagc gggggcaata ctcagcaaca gcaacagcgc cgtttcgggt
     2341 ggagaaaatg ccattgttaa cataacccgc accagcgatt cactcgcgca agttaaccgc
     2401 ggcactatta ccgcgaccaa cggctacagt gcgattaaaa cggcaagtac gggttctaac
     2461 agcaacggaa aatggatctg gaataccgat acgggtgtaa ttagcggcgt gaatcctaat
     2521 gcaccgttaa ttgatcttgg tcgcggctat aactttgcca acgcaggcac tattaatgtg
     2581 cagggtgatg gtgtagtcgc tatcagcggg ggaaccacca gctatacggt tcaactggtg
     2641 aacagtggga ccatcaatgt cggtacggcg cagggtaaag cggacggcac taacggtact
     2701 ggtttaattg gtatcaaggg gaatggccgt gagacgacaa ttaataacgc acaaagcggt
     2761 gtaattaacg tttacgcaga taactcctgg gctttcggag gacagactaa aacgatcatc
     2821 aataatggtg aaatcaacct gctgtgtgat atgggatgtg atatttatgc accgggcacc
     2881 accggtacac tgaacgatca taacagtact actgatatta ttgttcctgc cgcaacgagc
     2941 acacctacgc aggggtcagt acccacagta cctgcggact ccagtgcaca gcaaaaactg
     3001 actaactaca ctattggtac caatagcgat ggcacctctg gtatgttgaa agccaataac
     3061 cttgtcatca gcgacaacgt caaggtaaat accggattca gtgcaggtac tgccgacacg
     3121 actgttgtga ttaatgatgt tttcaaaggt gaaaatattt ccggggctga gaatatttct
     3181 tcttccacgg taatgtggaa tgctcagggc agcactgatg caagcggcaa cgtagacgtc
     3241 acgatgacca aaaacgccta tacggatgtt gtgacggatt cttcagttaa taatgtcgct
     3301 caggtactgg atacgggtta caccaataac gacctgtaca ctagtctgaa cgtaggaacg
     3361 actgctgaac tgaacagtgc gctgaaacaa attagcggca gccaggcaac aacggtattt
     3421 aatgaagcac gagtgttaag taaccgcttc agcatgttgt ccgatgcagc accggaagtt
     3481 gcaaacggtc tggccttcaa cgtggtagcg aaaggcgacc cgcgtgctga actgggtaac
     3541 gacacacagt acgacatgat ggcgctgcgt aaatctctga cgctgactga acatcagaac
     3601 ctgagtctgg aatacgggat tgcgcgtctg gagggtaatg gttcagatac cgcaggcgat
     3661 aacggagtga caggcggtta cagccagttc ttcggtctga aacaccagat ggcgtttgac
     3721 aacggtatga gctggaataa cgccctgcgt tatgatgtgc ataatctcga tagcagtcgc
     3781 tcgatagctt acggcgacgt caataaaacc gctgatgcta atgtgaaaca gcagtatctg
     3841 gaattccgta gtgaaggggc aaaaaccttt gaactgcgtg aaggtctgaa tgtgacacct
     3901 tatgcgggtg tgaaactgcg tcacacactg gaaggtggct atcaggaacg caatgcgggt
     3961 gactttaacc tgagcatgaa cagcggcagc gaaacggcgg tggacagcat cgttgggctg
     4021 aaactggact atgcagggaa agaaggctgg agtgcgaacg ccacgctgga aggtgggccg
     4081 aatctgagct atgtgaaaag tcagcgaact gcgagcattt cgggcgcggg aagccagcgt
     4141 ttcaacattg atgatggtca gaatggtggt ggtttcaaca gcctggcgac aatgggtgtg
     4201 aaatacagca gccaggagag tgcattgcag gtagatgcct tccactggaa agaagacggc
     4261 atcagtgata aaggtgtgat gctaaacttc aagaaaacgt tctaattttt agcatgtaat
     4321 acataaaccg caacgctgat acaggttgcg gtttttttat tgccggatgc ggtgcttgac
     4381 gtgtcttaac ctgtctgggg tttcgtgtct ttcaggacaa cagtacaact tatcccgcaa
     4441 tcttgtttac accctcgcat tatttatctc ttttcgtttc tatactgatt tttcttaatc
     4501 ggttttatta cagggcaggg tgcgatgagc agcaacacat ttactctcgg tacaaaatcc
     4561 gttaaccgtc ttggttatgg cgcgatgcaa ctggctggtc ctggggtttt tggcccccca
     4621 agagatcgcc acgtcgctat aaccgtgctg cgtgaggcgc tggcattggg cgtcaatcat
     4681 attgatacca gcgactttta tggtccgcac gtcaccaatc agattatccg cgaagcgctt
     4741 tatccgtact ctgacgacct gacaattgtc actaaaattg gtgcgcggcg tggagaggac
     4801 gcatcctggt tacccgcatt ttctccggca gagctgcaaa aagcggtaca cgataatcta
     4861 cgtaatctcg gcctggacgt actggatgta gttaacctgc gcgttatgat gggggatggt
     4921 catggcccag cggaaggatc gattgaggcc agcctgacag tgctggcgga gatgcaacaa
     4981 caaggcctgg taaaacatat tggcctgagt aacgtcacac cgacgcaggt tgcagaggcg
     5041 cgaaagattg ccgaaatcgt ctgcgtgcag aacgaataca acattgccca ccgtgctgat
     5101 gacgcaatga ttgatgcttt ggctcgtgat ggcattgcct acgtgccatt ctttccgctc
     5161 gggggcttta cgccgctgca atcctccacg ctttccgatg ttgctacaag ccttaatgcg
     5221 acaccaatgc aggtggcgct ggcgtggctg ttacagcgtt caccgaatat tttgctgatt
     5281 cctggcacct cttcggtggg gcatctgcgg gagaatatgg cgtccgatga attagtgcta
     5341 acggatgacg tgttggcaac gctggacagt atttcgcaaa aggcgggtta aataaaaaaa
     5401 cctcccactt tcgtgggagg catcataaat cttatgcagc aacaatgctg tcgatggctg
     5461 ctttcgcgtc agactgtgct ttcgctgcca tttccgggcc gtacgcgatc ccttcggcga
     5521 agacaaattt cacatcggta atgccgataa agcccaggaa tgtggacaga tacggcgtca
     5581 ccagatccgt tgggccatct ttatggatcc caccgcggct ggtgataacg atggcttttt
     5641 taccggtgac caggccttcc ggaccgttct ccgtatagcg gaaagtaacg cctgcgcgtg
     5701 caaccaggtc aaaatagttt ttcaactggg tcgagatatt gaagttatac atcggtgccg
     5761 caataacgat aacgtcgtgg gctttcagct cggcaatcag ctcatcggaa agcgccagag
     5821 cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagtgcacca accagttcgc
     5881 catccaggac cggaatcgga tttgcagcca ggtcacgcac ggtgatttca tcagcggagt
     5941 gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga gagtaccctg
     6001 ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat agatatttga
     6061 atgggcaatg ccccgttgct tgttgacact ttattcacaa ttctgccaca gagatagcgc
     6121 aataaatcga agcctatgtt cgaatttatt gaacaacgca tagaaagccg cgatgtggta
     6181 ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac taacgttatg
     6241 acagaacaac aaaaattgac ctttactgcc ttgcagcagc ggctggattc gatgatgctg
     6301 cgtgacagac tgcgtttttc tcgccgtctg cacggcgtga agaaggttaa aaatcctgat
     6361 gcacaacagg ccattttcca ggagatggcg aaagagattg accatgcggc agggaaagtc
     6421 ctgctgcgtg aagcggcacg accggagatt acttacccgg acaatttgcc ggttagtcag
     6481 aaaaagcagg acattctcga agcgattcgt gaccaccagg tggtgatcgt cgccggggaa
     6541 acgggttccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg gcgcgggatt
     6601 aaagggctga tcggccatac ccagccgcgt cgcctggcgg caagaacggt ggcgaaccgt
     6661 attgcggaag agctgaaaac ggagccgggc ggttgcatcg gttacaaagt gcgtttcagc
     6721 gatcacgtca gtgataacac gatggtcaag ctgatgaccg acggtatcct gctggcagag
     6781 atccagcaag accacctgct gatgcagtac gacaccatca ttattgacga agcgcacgaa
     6841 cgcagcctga atatcgattt tctcctcggc tatttgaaag agttgctgcc gcggcgtcct
     6901 gacctaaaaa tcattatcac ttccgcgact atcgacccgg aacgcttttc gcgccacttt
     6961 aataatgcgc cgattatcga agtctccggt cggacctatc cggtggaagt gcgctatcgc
     7021 ccgattgttg aagaagccga tgataccgag cgcgatcagt tgcaggcgat ttttgacgcc
     7081 gtagacgaac tgagccagga aagccctggc gacattctga tctttatgag cggcgagaga
     7141 gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac cgaaatcttg
     7201 ccgctttatg cacggctttc caacagcgaa cagaaccgcg tgttccagtc gcacagcggg
     7261 cgacgcattg tactggcgac caacgtcgcg gaaacctcgt tgaccgtacc gggtattaaa
     7321 tacgttatcg acccaggtac ggcgcgtatc agccgctaca gctatcgcac caaagtgcag
     7381 cgtttgccga ttgagccaat ttcgcaggct tcagctaatc agcgtaaagg ccgctgcggt
     7441 cgtgtgtcag aagggatctg tattcgtctc tattccgaag acgatttcct ctcgcgcccg
     7501 gagtttaccg acccagaaat tctgcgtact aacctggcct cggttatttt gcagatgacc
     7561 gcactgggct tgggcgatat cgccgcattc ccgtttgtcg aagcaccgga taaacgcaat
     7621 atccaggatg gcgtgcgtct gctcgaagaa ctgggcgcga tcaccacgga tgaacaggcc
     7681 agcgcctata aactgacgcc gctcggtcgc cagctctcgc aactgccagt cgatcctcgt
     7741 ctggcgcgta tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc gatgattatc
     7801 acgtccgcgc tctccattca ggatccgcgt gagcgtccga tggataaaca gcaggcatct
     7861 gacgaaaaac atcgtcgctt ccacgacaaa gagtctgact tcctggcatt tgtgaatctg
     7921 tggaattatc ttggcgagca gcaaaaggcg ctttcttcaa acgccttccg tcgcctgtgt
     7981 cgtactgatt atctcaacta tctgcgcgtg cgcgaatggc aggatatcta cacccagttg
     8041 cgccaggtgg tgaaagaact tggcattccg gttaacagcg aaccggcgga ttatcgcgaa
     8101 attcacattg cgttgctgac cggtttgctt tcgcatatcg gcatgaaaga tgccgataaa
     8161 caagaatata ccggcgcacg taacgcgcgt ttctccatct tccccggttc tggtttattc
     8221 aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg cctgtggggg
     8281 cgcattgcgg cacgcatcga tccagaatgg gtggaacctg tcgctcagca tttaatcaaa
     8341 cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc aacggaaaaa
     8401 gtcactgttt acgggttgcc gattgttgct gcgcgtaagg tcaactacag ccagatcgat
     8461 ccggcgttat gtcgtgaact ctttattcgc cacgcgctgg tggaaggtga ctggcagacg
     8521 cgtcacgcat tcttccgtga aaacctgaaa ctacgggcgg aagtagaaga gctggaacac
     8581 aaatcacgtc gccgcgatat tctggttgat gacgaaacgc tgtttgagtt ctacgaccag
     8641 cgcatcagcc acgatgtgat ttccgcacgt cacttcgaca gctggtggaa aaaagtcagc
     8701 cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga aggggcggaa
     8761 aaaatcagca aactggatta cccgaacttc tggcatcagg gcaatctcaa actgcgtttg
     8821 agctatcagt ttgagcccgg cgcggatgct gacggtgtga ccgtgcatat cccgctgccg
     8881 ttacttaacc aggttgagga gagtgggttt gagtggcaga tccccggtct gcgtcgtgag
     8941 ctgatcatcg ccctgattaa atcgctgccg aagccggtac gccgtaattt tgtgcccgcg
     9001 ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggaattgcc gttgctcgac
     9061 agccttgaac gagagttacg gcggatgact ggcgtcaccg ttgaccgcga agactggcac
     9121 tgggatcagg tgcccgatca cctgaaaatc actttccgcg tggtggatga caaaaacaag
     9181 aagctaaaag aagggcgctc attacaggat ctgaaagacg ccctgaaagg caaagtgcag
     9241 gaaacgctgt ctgccgtggc ggatgacggt atcgagcaga gcggtttaca tatctggagt
     9301 tttggtcaac tgccggaaag ctacgagcag aagcgcggca actacaaagt gaaggcgtgg
     9361 ccggcgctgg tggatgagcg tgacagcgta gcgatcaaac tgtttgataa cccgctggaa
     9421 cagaagcagg caatgtggaa cggtcttcgc cgtttactgc tgctaaatat tccatcgccg
     9481 atcaagtact tgcacgaaaa gctgccaaac aaagccaagc tggggctgta ctttaatccg
     9541 tatggcaaag tgctggagct gatcgatgac tgtatctcct gcggtgtgga tcaactgatc
     9601 gacgccaatg gtggcccggt ctggacggaa gaaggctttg ctgcgctgca tgaaaaagtc
     9661 cgcgctgaac tgaacgacac ggtggtggat attgcgaagc aggtcgagca aatcctcact
     9721 gcggtgttca atatcaacaa acgtctgaaa gggcgggtgg atatgaccat ggcgctgggg
     9781 ctttctgaca ttaaagcgca gatgggcggg ctggtgtatc gcggttttgt cactggcaac
     9841 ggttttaaac ggttgggcga cacgttacgg tatttgcagg cgattgaaaa acgactggaa
     9901 aaactggcga ttgatccgca tcgtgaccgt gctcagatgc tgaaagtcga aaacgtccag
     9961 caggcatggc agcaatggat caacaaactg ccgcctgcac gtcgtgagga tgaagacgtg
    10021 aaagaggtcc gctggatgat agaagagctg cgcgttagtt acttcgctca acaacttggt
    10081 acgccttatc cgatttcgga taagcgtatt ttgcaggcga tggagcagat tagcggttaa
    10141 cact
//
LOCUS       DADSZD010000157        10096 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.158, whole genome shotgun sequence.
ACCESSION   DADSZD010000157 DADSZD010000000
VERSION     DADSZD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.158"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..767)
                     /gene="trpA"
                     /locus_tag="J1Z28_003598"
     CDS             complement(<1..767)
                     /gene="trpA"
                     /locus_tag="J1Z28_003598"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBA7718398.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAAL"
     gene            complement(767..1960)
                     /gene="trpB"
                     /locus_tag="J1Z28_003599"
     CDS             complement(767..1960)
                     /gene="trpB"
                     /locus_tag="J1Z28_003599"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBA7718399.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(1972..3330)
                     /gene="trpCF"
                     /locus_tag="J1Z28_003600"
     CDS             complement(1972..3330)
                     /gene="trpCF"
                     /locus_tag="J1Z28_003600"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBA7718400.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLSI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(3334..4929)
                     /gene="trpD"
                     /locus_tag="J1Z28_003601"
     CDS             complement(3334..4929)
                     /gene="trpD"
                     /locus_tag="J1Z28_003601"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBA7718401.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(4929..6491)
                     /gene="trpE"
                     /locus_tag="J1Z28_003602"
     CDS             complement(4929..6491)
                     /gene="trpE"
                     /locus_tag="J1Z28_003602"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBA7718402.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPEGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            complement(6583..6627)
                     /gene="trpL"
                     /locus_tag="J1Z28_003603"
     CDS             complement(6583..6627)
                     /gene="trpL"
                     /locus_tag="J1Z28_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBA7718403.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6765..7646
                     /gene="yciV"
                     /locus_tag="J1Z28_003604"
     CDS             6765..7646
                     /gene="yciV"
                     /locus_tag="J1Z28_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBA7718404.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7643..8263
                     /locus_tag="J1Z28_003605"
     CDS             7643..8263
                     /locus_tag="J1Z28_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBA7718405.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8291..>10096
                     /locus_tag="J1Z28_003606"
     CDS             8291..>10096
                     /locus_tag="J1Z28_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HBA7718406.1"
                     /translation="MAGIYRCILLLIVGLFFSSLSYAKNTEIPSYEEGISLFDVEATL
                     QPNGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTLFSLKLPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLGNAPAPQTTNIFSHLLLPSTSSLLICFPSLFLACGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDHYDKNQQILIRV
                     NEGATRNNAVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKSSF
                     YRPDAFLQWGGMAILAVILYGNLSPVGWAGMSLVGDMFIMICWLLTFLFCSLDLLFAR
                     DDDKPCVNRVIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGM
                     GYIFLPKFTQNGQQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSAN
                     LGREWAARITPSLTAAVRAPEIARSGVLFSLQMHLS"
BASE COUNT         2362 a         2629 c         2649 g         2456 t
ORIGIN      
        1 ttcagtgccg ccagcatttt ctctggctca ttaatatgtt gctcgatgat tttaacgatg
       61 gccgaaccag aaatcgcgcc cgcagctcct gcatcaatcg ctgcttttac ctgatccggg
      121 gcggaaatac caaatccctg caatggaggc gcagcattgt attctttcag cttcgtgacc
      181 agatgattga ggggtaacgc ggcgcggttt tctgcgccag tcacgcccgc acgcgacagc
      241 aaataggtgt atccacgacc gtaggaggct atctggcgca gcaaatcatc gtcggcattc
      301 ggcgggcaaa taaagatagg tgcgacatta tgacgcaacg cggcctggcg gaagggcgcg
      361 gactcttcca cgggcacatc ggcaaccagc accgaatcga cgccgacttt ctcgcactcg
      421 gcataaaact catcaatgcc tttgttaaac accaggttgg catacatcaa aaggccgatg
      481 ggaatggtcg ggtgcttctg gcgaatgagt gccagcatct caaagcactg cgccggggtt
      541 actcccgccg caaaagcacg cagtgtggcg ttttgaatcg tcgggccatc cgccagtggg
      601 tcggagaagg ggatgcctaa ctccagcgca tcagcaccgg cttcaattag cgtatcgata
      661 attttcaacg actgctcaat gcccggatca ccgagggtga cgaaaggaac gaatgcgcct
      721 tctttgcgct ccttcaactg ggcaaacaga gattcgtagc gttccatcag atttcccctc
      781 gtgctttcaa aatatcgtga acggtgaaga tgtctttatc gccgcgaccg gaaaggttaa
      841 ccaccagtag ctgctctttt tccggatttt cgcgcatcat tttcagcgca tgggccaggg
      901 cgtgggagga ttccagcgcc gggatgatcc cttcatgcag gcaaagcgtt ttaaaggctt
      961 ccagggcttc atcgtcggta atagacacgt aatcagcgcg tccagtgctg ttgagatacg
     1021 catgttgcgg gccgacggac gggaaatcca gcccggcaga aatggagtaa gactcttcaa
     1081 tctgcccgtc ttcggtttgc atcatcggcg ctttcatacc gaaatagatg cccacgcgac
     1141 catgttttaa cggtgcgccg tgctcgccag tttcgatacc gtggccgcca ggctccacac
     1201 caatcaggcc gacgtcggtt tcgttgatga aatctgcaaa cataccgatg gcattcgaac
     1261 caccgccaac acaggcgata acggcatccg gcaggcgacc ttctctttcc agaatctgcg
     1321 ctttcgtttc ttcgccaatc atccgctgaa actcacgcac aatggtcggg taaggatgcg
     1381 ggccagctgc ggtacccagc atatagtgcg cggtttcata actgccggac cagtcgcgta
     1441 gcgcctcatt acaggcatct ttcagcgtcg cggaaccgct atgcaccggg atcacttccg
     1501 cacccattaa gcgcatccgg aaaacgttgg gcgactggcg ttcaacgtct ttggcaccca
     1561 tataaatacg gcatttcagg ccgagcaggg cgctggcaag ggccgacgcc acgccatgct
     1621 gaccggcacc ggtttcggca ataatttcag ttttacccat ccgcttcgcc agtaaagcct
     1681 gaccgagcac ctggttagtt ttatgcgcgc cgccgtgcag caaatcttcg cgcttcagat
     1741 acagcgtggt gttcgtcccg gctgtaatgt tctggcattt ggtcagcgcg gttggacgcc
     1801 cggcatagtt tttcagcagg tcgttgaact gagcctgaaa ttcaggatct ttttgcgcgc
     1861 tgacaaaagc ttcttccagc tggcgcagag caggcatcag gatttgtggc acgtacatgc
     1921 cgccaaactc accaaaatag gggttaagta atgttgtcat tgttcctttc cttaatatgc
     1981 gcgcagcgtc tggaaaaccg aggccaaaag acgtgcgtct ttgatacccg gttgcgactc
     2041 tacagcagaa ttaaaatcaa gcccggcgca gccggtttgt gccgcttcca cgcagttatc
     2101 tgcgcctaag ccccccgcca gcagaacgtt gccaagcgat tgaccattta atagtgacca
     2161 gtcgaaacgt tgtccgctcc cgccctgacc gttgtcgaat acatatttat cgatgtgctg
     2221 aaaatcgcgc gcgggaagag tttcaccgac acttaaagcc ttccagatgg cgacgtgtgc
     2281 tggcagagcc tcacgcagag tgtcgatata cagctgatct tcattaccat gcagttgcac
     2341 tgccgccagc gataacacct tagctttgtc caccacatcg gcaatatcgt gattgcggaa
     2401 cacgccaaca tactgcaacg gtgccgcagc catcacttcc tgcgcctgtt caacgttgac
     2461 gcaacgcggt gatgtcgcaa caaaaatcaa cccaccgtaa atcgcgcccg cgtcataagc
     2521 tgctttagca tcttgcccac gtgtcaggcc acatacttta ttctcaccca gcaacacccg
     2581 acgcacggcg gcgttcaaat cgtcatgggc catcaacgcc gaaccaatca gaaagccgtt
     2641 agcgaagtgg cttaactcgc gcacctgagc gtaagtattg atgccggatt cgctgattac
     2701 cgtcacgttg tgccccagtt tcggcgcaag ctcgcgggta cggttgagat caatcgacaa
     2761 atcgcgcaga tcgcggttgt tgatgccaac gacctttgcc cccaatgcaa tggcgcgctc
     2821 cagttcctct tcattactga cttcggtcag cacacccatc tccagactgt gggcgacggc
     2881 tgcaagctgg cgatattgtt cgtcatccag tactgaaagc attaataagc aggcatcggc
     2941 ctggtaatag cgcgccagat agatctggta aggatcgata atgaagtctt tacataaaat
     3001 cggctgcggg gcgatttggc tgacgatgga gaggaaatca aagctcccct gaaaatattt
     3061 ctcatcagtc agcactgaaa ttgccgaagc gtaatgttta taaatggcag caatgcgtgc
     3121 cggatcgaaa tcatcacgga tcacgccttt tgacggcgac gcttttttgc actccagaat
     3181 aaacgccgtg cgtgcaccct gtagcgcatc ataaaaatgt cgcgtgctcg gctgaatctc
     3241 attctgaaaa ctggccagcg gttgctgctg tttgcgggct tctacccaaa tcgccttgtc
     3301 tgcgacgatt ttcgctaaaa cggtttgcat tatttaccct cgtgccgcca gtgcggtgac
     3361 tctgtcgtaa gcggaaccac tgcgcagtac ctcaagaacg gtttgcgcat tggcttgcag
     3421 atcttcatgg ccatgcaggc gcattaacat ggcgacattc gccgcgacgg ctgcttcgtg
     3481 agcggcgtcg cctttacctt gtaacaagcg tgttaaaatg tcacggtttt cttccggtgt
     3541 tccgcctgcc agttgctcct ggtggtaggg tgtcaggcca aaatcttcag cggtcaactg
     3601 atagctctta atttcgccgt catgtagttc ggcaacgatt gtcggcgcgt gtaatgaaac
     3661 ttcatccatc ccgccgctgt gcaccactgc cgcgcgttga taccccagca cgcgcaaggt
     3721 ttcggcaatc ggcagcacca gctccgggct gtagacacca attaacgcca gcggcggatg
     3781 cgccgggtta attaatggcc ccagcacatt gaacagtgtg cgggttttca gttgctggcg
     3841 aaccggcatt gcatggcgga atccggtgtg atatttcggt gcaaagagga aacagacgcc
     3901 taactcatcc agcgcctggc gcgatttatc ggcgttcata tcaagattaa taccgaacgc
     3961 cgccagcaga tccgacgagc cggatttact ggagacgcta cggttgccgt gtttcgccac
     4021 tttcagcccg caggccgcgg cgacaaacgc gctggcggta gaaatattga tgctgttgct
     4081 gccgtcaccg ccagtaccga cgatatcggc aaacagataa tccgggcgcg ggaacggcgc
     4141 ggcgttttcc agtagcgcgg ttgccgcccc ggcgatctcg ttcgggtgct caccgcgaat
     4201 tttcatgctc accagcgccg ccgccagttg ttccggtttc agttcgccac gtaccaccgc
     4261 tgaaaacagc tggtggcttt cttgttggct aagcgtctgt gcctgataca gtttttccag
     4321 aatcggttgc agcgtgttgg ttggctcaag tttctgctgc gcccaggcca gcgtttgttc
     4381 cagcaggcga gcgccctggg tagtaagaat ggattccgga tggaactgga atccacaaac
     4441 gcgatccgca tcatgacgca ccgccatcac catgccatta aaatgggcgt tgatggttaa
     4501 accggccgga atattactgc caaccagcga gtgataacgc gccactggca gcgggtttgt
     4561 taatccggca aacatcgcct gaccgtcatg ttcaatgctc gacgctttac cgtgaagaat
     4621 ttcgcccgcc tgaccgacat agcccccgta agcttcgaca atcgcctgat gtccgaggca
     4681 aatgccaata attggcagct tgccacgcaa gcgggtgagg agttccggca tacaaccggc
     4741 ttcgctcggc acaccggggc caggagaaag catcagcacc ggattgctca tcgtcgccag
     4801 gcgttcaatt aaggtctgcg ccggaatatg gttgcggtaa atcaccacgt tatgaccatt
     4861 gctgcgcaac tgatctgcca ggttgtacgt aaaagagtcg atattatcga gcagcagaat
     4921 gtcagccatt agaacgtctc ctgtgcatga tgcgcggtgg caatagcgcg cagtacagcg
     4981 cgggctttat tacgagtttc gtcggcttcc gactgcggaa cagaatcaag gactacgcca
     5041 gcaccggctt gcacggtggc gataccgttt tccaccagcg ccgagcggat cacaatgcag
     5101 gtgtcgagat cgccatgcgc ggtaaaataa cctaccgcgc cgccgtagct gccgcgtcga
     5161 cgaccttctg cttcggcaat taactgcata gcgcgtactt tcggcgcacc gcttaacgtc
     5221 cccatattca tacaggcgcg gtaagcgtgc agggcgtcga gatcgtggcg cagctcacca
     5281 acaacgcggg agaccaggtg catcacgtaa gagtaacggt caactttggt gagatcggcg
     5341 acgtagcggc tgccgggggt gcaaatacgc gccaggtcat tacgggcgag atccaccagc
     5401 atcagatgtt cagaaagctc tttatgatcg gtgcgcattt ccagttcgat gcggctgtcg
     5461 aggtctctat ccagtgaacc atcggcacga cgaccgcgtg gacgtgttcc ggcaatcggg
     5521 taaatctcaa tctggcggct ggtggcgtca tacttgagcg aactttccgg cgacgcgcca
     5581 aacagggtga aatcattatc ctgcataaaa aacatgtacg ggctgggatt actctttttc
     5641 agcacgtaat aggccgccag cggtgacggg cagggcagag agaaacggcg agatggcacc
     5701 acctggaaaa tttctccggc gcgaatcgct ttttgcaaca aacgcactac gccaccgaac
     5761 tcttcatcgc tctggttaca ttcacaacgc atatgcggca cggaaaccac cggcagcggc
     5821 ggcgcggctt cggtcagttg ctgacgtagt tcgttcaggc gagcagtgag acgttgtttt
     5881 tcttcttcat tcggagcaaa caggctggcc tgaatacggg tgcttttttt ctgatggtca
     5941 atcaccatca gcgtttcagc gagataaaaa cagaaatcag ggcagctatt ttccgctgac
     6001 agttgcggta aattttcaaa tcccgccaca aggtcataag agaacaggcc gccgaagaac
     6061 attgcttctc gttcttcctt cggtacattc aacagattct gtaataagcg gaaagcgtca
     6121 aaaaccgaaa gggagcataa gcgggcgtct tcatccagca gtggactgac aggcgggaag
     6181 cgcagtacgc ggcagtttgg tgattgttca ttttccacac cctcaggcaa ggcgttatcc
     6241 agtagtgtca acagagcttc tccattgccg gaaagtgcct ggattgtgac agtgtcactt
     6301 aatgctgtaa tgcgcagcgc actgtctacc agcagcaggc tttttaaatc atctttgctg
     6361 tcgatatctg cggattccag cagcagcgtt gccggacgat ccccacacaa ctggtgaaaa
     6421 agcgcagtcg ggttgtcgcg ataagcgcct ttgcaggtta gcagttcgag agtcggtttt
     6481 tgtgtttgca ttgttattct ctaattttgt tcaaaaaaaa gcccgctcat taggcgggct
     6541 gggtatctga ttgctttacg catggtgaat acactgcccg tttcaggaag tgcgccacca
     6601 acctttcagt acgaaaattg ctttcattgt cgataccctt tttacgtgaa cttgcgtact
     6661 agttaactag ttcgatgatt aattgtcaac agctcatttc agaatatttg ccagaaccgt
     6721 tatgatgtcg gcgcaaaaaa cattatccag aacgggagtg cgccttgagc gacacgaatt
     6781 acgcagtgat ttacgacctg cacagccata ccacagcttc cgatggctgc ctgacgccag
     6841 aagcattggt gcaccgtgca gtcgagatgc gcgtcggcac cctggcgatc accgaccatg
     6901 acaccacagc tgcaattgca ccagcaagag aggaaatttc acgttcggga ctggcgctga
     6961 atcttattcc tggtgtggaa atttccacgg tctgggaaaa tcatgaaatt catattgtcg
     7021 ggctgaatat tgatattact catccgctaa tgtgtgagtt cctcgcgcag cagacagaac
     7081 ggcgcaatca gcgggcgcag ctgattgccg aaagacttga aaaagcgcaa atacctgggg
     7141 cactggaagg cgcgcaacga ctggcgcagg gcggtgcggt gacgcgcggt cattttgcgc
     7201 gtttccttgt ggagtgcgga aaagccagtt caatggcgga tgtctttaaa aagtatctgg
     7261 cgcgcgggaa aaccggatac gttccgccac agtggtgtac aatagagcaa tctattgatg
     7321 tcattcatca ttctggcggt aaggcggtgc tagctcatcc tgggcggtac aatcttagtg
     7381 ctaaatggct gaaaagattg gttgcgcatt ttgccgaaca ccacggcgac gcgatggaag
     7441 tcgcgcagtg ccagcaatcg cccaatgaac gcacccagct ggcgacactt gcgcgtcagc
     7501 atcatttatg ggcatcacaa ggatctgatt ttcatcagcc atgcccgtgg atcgaactgg
     7561 gtcgtaaact ctggctgccc gcaggcgttg aaggcgtctg gcagctatgg gaacagccgc
     7621 agaacaccac agagagggaa ttatgagcca gtttttttat attcatcctg ataacccgca
     7681 gcaacgcctg atcaaccagg cggtggagat cgtgcgtaaa ggcggggtga ttgtttatcc
     7741 aactgattcc ggctatgcgc tcggctgtaa aattgaagat aaaaacgcga tggagcgtat
     7801 ttgtcgtatt cgccagctgc cggacggtca caactttacc ctgatgtgtc gcgatctttc
     7861 tgaactctcg acctattcat ttgtcgataa cgttgcgttt cgtttgatga aaaacaacac
     7921 gccaggcaac tataccttca tcctgaaggg gacgaaggag gtgccacgcc gtctgttgca
     7981 ggaaaaacgc aaaaccatcg gtatgcgcgt gccgtctaac ccgattgcgc aggcgctgct
     8041 tgaagcattg ggcgaaccga tgctttccac ttcgctaatg ctgccaggca gtgaatttac
     8101 cgaatccgat ccggaagaaa ttaaagaccg tctggaaaaa caggtggatt tgattattca
     8161 cggcggctat ctcgggcaga aaccaacaac ggttattgat ctcaccgatg atacgccggt
     8221 cgtggtgcgt gaaggcgtag gtgatgtgaa gcctttctta taacaaattg cgttccacgg
     8281 atggaaaatg atggcaggga tatatcgctg cattttgctg ttgatagttg ggcttttttt
     8341 ctcttcgttg agttatgcga aaaacacgga gatcccttct tatgaagaag ggatctcgct
     8401 ctttgatgtt gaagccactc tgcaaccgaa tggggtgctc gacatcaaag agaacattca
     8461 ttttcaggcg cgaaatcagc agattaagca cggattttat cgtgatttac cgcgactctg
     8521 gatgcagcct gatggggacg ctgcactgct gaactatcat attgttggcg tcacccgtga
     8581 tggtattcct gaaccctggc atcttgactg gcatatcggg ttaatgagta ttgtcgtggg
     8641 cgataaacaa cgtttcttgc ctcaaggcga ctatcattat caaattcatt atcaggttaa
     8701 aaatgctttc ctgcgtgagg gggattctga tctgctaatc tggaacgtga ccggtaacca
     8761 ctggccgttt gaaatttata agaccctatt ttcactcaag ttgcctgata ttgcgggtaa
     8821 tccatttagc gaaatcgatc tctttactgg agaagagggc gacacatatc gaaatggccg
     8881 catccttgag gacggaagaa ttgaatctcg cgatccgttt tatcgtgaag atttcacggt
     8941 cctctaccgc tggcctcacg cattacttgg taatgccccg gctccacaaa cgacgaatat
     9001 tttcagtcat cttcttttac cctctacgtc atcgttgtta atttgctttc cgagtctgtt
     9061 cctggcgtgt ggatggttat atctctggaa gcgcaggccg caatttacgc cggtagatgt
     9121 gattgaaacc gatgtcattc cgccagatta cacgcccggc atgttacgtc ttgatgcgaa
     9181 actggtttac gacgataaag gtttttgcgc cgatatcgta aatctgattg tgaaaggaaa
     9241 aattcatctg gaagatcatt atgacaagaa ccagcaaatc ctgattcgtg ttaatgaagg
     9301 cgcgaccaga aataatgcgg tattactgcc cgcagagcag ttattactgg aagcgttatt
     9361 tcgtaaaggc gataaggtcg ttcttacggg gagacgcaac agagtcttac gcaaggcatt
     9421 tttacggatg cagaaatttt atctgccgcg taaaaagtct tcgttttacc gacctgatgc
     9481 gtttttgcaa tggggcggaa tggcaatatt ggcggtcatt ctctacggta acctgagtcc
     9541 cgtaggctgg gcaggaatga gtctggtagg cgatatgttt attatgatct gctggcttct
     9601 tactttctta ttttgttccc ttgatctttt gtttgcccgc gatgatgaca agccttgcgt
     9661 taatcgtgtc atcatcactt tgtttttacc actgatttgt tcaggtgtgg ctttttactc
     9721 tctctacatc aatgtcggag atgtattctt ttactggtat atgccagcgg gttattttag
     9781 cgcggttttc ctgaccggtt atctcactgg catggggtat atttttctgc caaagtttac
     9841 ccaaaacggg cagcaacgtt atgcccacgg tgaagctatc gttaactatc ttgcgcgtaa
     9901 agaggcggca acacacagtg ggcggcggcg gaaaggggaa acacggaaac tggattacgc
     9961 tttgttaggt tgggcagttt cggcaaacct tggcagagaa tgggcagcac gtatcacccc
    10021 atcactcaca gcggctgttc gcgccccgga aattgcccgt agtggcgttt tgttctcatt
    10081 acagatgcat ctgagt
//
LOCUS       DADSZD010000158        10018 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.159, whole genome shotgun sequence.
ACCESSION   DADSZD010000158 DADSZD010000000
VERSION     DADSZD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.159"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            239..1009
                     /locus_tag="J1Z28_003607"
     CDS             239..1009
                     /locus_tag="J1Z28_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBA7718407.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNQDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1000..1473
                     /locus_tag="J1Z28_003608"
     CDS             1000..1473
                     /locus_tag="J1Z28_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBA7718408.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1581..2120
                     /gene="dnaT"
                     /locus_tag="J1Z28_003609"
     CDS             1581..2120
                     /gene="dnaT"
                     /locus_tag="J1Z28_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBA7718409.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFDNNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQTAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2123..2860
                     /gene="dnaC"
                     /locus_tag="J1Z28_003610"
     CDS             2123..2860
                     /gene="dnaC"
                     /locus_tag="J1Z28_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001731874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBA7718410.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2909..3403
                     /locus_tag="J1Z28_003611"
     CDS             2909..3403
                     /locus_tag="J1Z28_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBA7718411.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNVAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3657..5948
                     /gene="opgB"
                     /locus_tag="J1Z28_003612"
     CDS             3657..5948
                     /gene="opgB"
                     /locus_tag="J1Z28_003612"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBA7718412.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSRTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6088..7110)
                     /locus_tag="J1Z28_003613"
     CDS             complement(6088..7110)
                     /locus_tag="J1Z28_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HBA7718413.1"
                     /translation="MSTMKVLICQEIKNLVWEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7249..8154
                     /locus_tag="J1Z28_003614"
     CDS             7249..8154
                     /locus_tag="J1Z28_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7718414.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARKPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(8325..9977)
                     /locus_tag="J1Z28_003615"
     CDS             complement(8325..9977)
                     /locus_tag="J1Z28_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBA7718415.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         2630 a         2570 c         2374 g         2444 t
ORIGIN      
        1 tatctattat cctgactcgt aaaggccaaa taacaaattc attatagtga taatattcat
       61 gcgatagttt gtttacggcg agagataatt cgctttttat caccgagcat ctcccgataa
      121 ccaggattaa tcagcgcaat tttgatctta acctaccgca aaacaaccgt gcttcatgat
      181 acaaatggat tctcattatc tgaacaatac gcagcacgtc tatgacaaag ggcgagttat
      241 gcaaactgag caacagcgag ccgtaacacg gctttgtatc cagtgtggat tatttctttt
      301 gcaacatggt gcggaaagcg cgttggttga tgagctttcc tcacgactgg gtcgggcgtt
      361 gggaatggac agcgtcgaaa gttctatctc ttcgaacgcc atagtgctga caaccattaa
      421 agatgggcaa tgcctgacat cgacacgtaa aaatcaagat cgcggcatta atatgcatgt
      481 ggtgactgaa gtccagcaca ttgtgattct ggcggagcac catctgctgg attacaaagg
      541 cgtagagaaa cgatttagcc aaattcagcc attacgttac ccaagatggc tggtagcctt
      601 aatggttggc ctttcctgcg cctgtttctg taaactcaat aaaggtggct gggatggtgc
      661 cgtcatcacc ttctttgcca gtacggccgc gatgtatatc cgccagctgc tggcacaacg
      721 tcatcttcat ccacagatca acttttgcct caccgctttc gccgccacca ccatttccgg
      781 attgcttttg caacttccca ctttcagcaa tacccccacc attgcgatgg ccgccagcgt
      841 tctgctgctg gtgccgggct ttccgttgat aaatgccgtc gccgatatgt ttaaaggcca
      901 catcaatacc ggactggcac gctgggcaat cgccagtctg ctgacactgg ctacctgtgt
      961 cggcgtagta atggcactga cgatttgggg gctacgcgga tgggtgtgat cgaatttctg
     1021 ttagcgttgg cgcaggatat gatcctcgcc gccattcctg cggtcggctt tgcgatggtg
     1081 ttcaacgttc ccgttcgagc gttacgctgg tgtgcgctgc ttggtgcgat aggtcatggt
     1141 tcccgaatga tcttaatgac cagcgggctg aatattgaat ggtcaacctt tatggcctct
     1201 atgctggtcg gcaccattgg tattcaatgg tcgcgctggt atctggcgca tccgaaagtg
     1261 tttaccgtgg cggctgttat ccctatgttc cctggcatat cagcttatac cgcaatgatt
     1321 tcggcggtaa aaatcagcca gctaggttac agcgaaccgt tgatgattac cctgttaacc
     1381 aactttctta cagcttcatc gattgttggt gcgttatcca tcggtctttc cattcctgga
     1441 ttatggttgt accgcaagcg ccctcgcgta taaaattgcc tcacttaaag cacgcggatg
     1501 agagacagcc tcctctcctc cgtgtgttac tataaaagtt atctcccttc tcgttcatcg
     1561 ttccatattt gagaaacagt atgtcttcca gagttttgac cccggacgtc gttggtattg
     1621 acgccctggt acacgatcac caaaccgttc tggcaaaagc tgaaggcggt gtggttgccg
     1681 tatttgacaa caacgccccg gcgttttatg ccgtcacgcc tgcacgcctg gctgaactgc
     1741 tggcgctgga agaaaagctg gcgcgtccgg gaagtgacgt ggccctggac gatcaactct
     1801 atcaggaacc gcaaaccgct cccgttgccg tacccatggg gaaatttgcc atgtatccgg
     1861 actggcaacc cgatgccgac tttatccgcc tggcggcgct atggggcgtg gcactaagag
     1921 agccggtgac cgccgaagaa ctggcgtcat tcattgccta ctggcaggcg gaaggcaaag
     1981 tctttcacca tgtgcagtgg caacaaaaac tggcgcgcag cctgcaaatc ggtcgtgcca
     2041 gcaacggcgg actgccgaaa cgagatgtga atacggtcag tgaacctgac agccaaattc
     2101 caccaggatt cagagggtaa cgatgaaaaa cgttggcgac ttgatgcaac gcctgcaaaa
     2161 aatgatgcct gcccatatca aacctgcttt caaaacgggt gaagaattac tggcctggca
     2221 gaaagaacaa ggggcgatcc gctccgccgc tctcgaacgt gaaaatcggg cgatgaaaat
     2281 gcagcgcacc tttaaccgct ccggtattcg tccactgcat cagaactgct cctttgagaa
     2341 ctatcgcgtt gagtgtgaag ggcaaatgaa tgcgttaagc aaagcgcgcc agtatgtcga
     2401 agagtttgac ggcaacatcg ccagcttcat cttttctggc aagccaggaa ccggcaaaaa
     2461 ccatctggcg gcggcaatct gcaacgagct gctgctacgc ggtaaatccg tattgatcat
     2521 caccgtggcc gatattatgt cggcgatgaa agataccttc aggaatagcg gtaccagcga
     2581 agaacaactg cttaacgatc tgagtaacgt tgatctgctg gtgatcgatg agatcggcgt
     2641 gcagaccgaa tcgaaatacg aaaaagtgat catcaaccag atcgtcgatc gccgctcttc
     2701 ttccaaacgc ccaaccggga tgctgaccaa cagtaatatg gaagagatga ccaagttgct
     2761 gggcgaacgc gtgatggacc gtatgcgcct gggtaacagt ttgtgggtga tcttcaactg
     2821 ggatagctac cgtagccgtg taacaggtaa agagtattaa tccccgtttc tggcggtata
     2881 ctgcgacaca attcaacctt gagtaatgat gatgaaaact attaaacatc ttctgtgctg
     2941 tgccattgcc gccagcgcat taatttccac cggggtgcat gctgcgtcct ggaaagatgc
     3001 gctctccagc gccgccagcg aacttggcaa ccaaaacaac acgacacagg aaggcggttg
     3061 gtcgctcgcg tcattaacta acttgcttag cagcgggaac caggccttaa gcgcagataa
     3121 catgaacaac gtcgcaggca ttctgcaata ctgcgcgaag caaaagctgg cttcggtaac
     3181 cgatgccgaa aacatcaaga atcaggtact ggaaaaactg ggcctgaaca gtgaagagca
     3241 aaaagaagac accaactatc tggatggcat tcagggtttg ctgaaaacca aagatggtca
     3301 gcaactcaat ctggataata ttggaacgac tccgctggca gaaaaggtga aaaccaaagc
     3361 ctgcgatctg gtgttaaagc aggggctgaa cttcatttcc tgaaccgcga aacgccgcgt
     3421 tttagctgtt acccggctga cgcggctctc tcctgccctt ttccctgctc ctggacggtt
     3481 ttacccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa actaattcct
     3541 aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc attacattgt
     3601 gcttctcaaa aaacttaaac gttggctaaa cggcccggtt tagtgaggat cgcccgttgt
     3661 cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca tggaaagcgg
     3721 gacgtaacac ctggtggttt gcggccacat taacggtact ggggctattt gtcgttttaa
     3781 atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac gcggttctct
     3841 ataccttaac caacagcctg accggtgctg gtgtcagcaa atacatcctg cctggtatcg
     3901 gcattgtgct ggggctggcg gcggtgttcg gtgcgctggg ctggatcctg cgccgtcgtc
     3961 gccatcatcc gcaccatttt ggttacagcc tgctggcact cttactggcg ctgggttcag
     4021 tggatgccag cccggcattt cgtcagataa cggaactggt gaaatcccag tcacgcgacg
     4081 gcgacccgga ctttgcggct tattataaag agccgtcgag aactatccct gacccgaaac
     4141 tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat aacgaggctt
     4201 tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat ttcagccaca
     4261 cgcagcagct gccgggaacg gattacacga ttgcgggcat ggtagcttct cagtgcggca
     4321 taccgctgtt tgccccattt gaaggcaacg cctccgcctc tgtctccagc ttcttcccgc
     4381 agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat ttcgtgcagg
     4441 gcgcgaatct gcgttttgcc ggtaaagatg tgttcctgaa gtcgcatggc ttcgaccact
     4501 tatacggctc agaagagctg aaaagcgtgg tggccgaccc gcactatcgc aacgactggg
     4561 gattctacga cgataccgtt ctcgatgaag cgtggaaaaa atttgaagag ctttcccgct
     4621 caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg gatggtttta
     4681 tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat cagtcattca
     4741 gcgcggtaag ttgcagccag gagaatattg cgacgtttat caacaaaatc aaagcgtcac
     4801 cgtggtttaa agataccgtc atcgtcgtct cttctgacca tttagcgatg aacaatacgg
     4861 cgtggaaata ccttaataag caggatcgca ataacctgtt ttttgtcatc cgtggcgaca
     4921 agccgcagca agagacgctg gcagtgaagc gtaatacgat ggataacggc gcgacggtgc
     4981 tggacattct cggtggcgat aactatctcg gacttggccg tagcagttta tccgggcagt
     5041 cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag ccggatatca
     5101 tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag cagaaaaaca
     5161 tgattgcctt ctcaggtagc catttccgtt tgccgctgct gttgcgggtt tcagacaaac
     5221 gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag ctggcagatt
     5281 tcgctccacg cgacaatttc gtctgggttg accgttgcta caagatggca caactctggg
     5341 ctccggaact ggcactctcc accgactggt gtgtctcgca agggcagctt ggcggtcagc
     5401 aaattgttca gcatgttgac aaagcaatat ggaagggaaa aacggcattt aaagatacgg
     5461 tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct aaagattgtt gataacgata
     5521 ttcgctacaa agccgacagt ttcatcttta acgtcgctgg tgcgccagaa gaggtgaaac
     5581 agtttagcgg gatttctcgt ccagagtcgt ggggccgctg gtccaacgca cagctgggcg
     5641 atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg gtgattaccg
     5701 ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc aatgaagaac
     5761 aaacccttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat aacccaaccg
     5821 atgccgacac actggtaatt gtgccaccgg aacctgtctc aaccaacgaa gggaatatcc
     5881 tcgggcactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg gtagaacgtg
     5941 aagggtaatt cctgactgat gtgtaggtcg cataaggtgc gacctacaca ccttctccgg
     6001 cctacaaaac attattgaga aacagaaaaa aaacagcgcc caacacttcc ccggacgctg
     6061 catggcaact tgaataatct cgccttatca gaaagtaatt acgcccttaa tcaactcacg
     6121 attgttaatc acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt
     6181 taacatcatg tcggcagtga ttttcccttc cgccatcaat cgaccgactt tagcgaaatc
     6241 ttccggcgtg gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc
     6301 ggagaactgc aactcacctt taaacaggcc gacaaatacc accgtgccgc cgtgacgaat
     6361 cagatttacg gtgttattca tcgcatgttg attacctgtc gcgtcaatca ctttctgcgc
     6421 cagcgaacca ccaaactgcg cccgcagctg cgcgtcaaaa tcttcggctg acgggtccag
     6481 aacaggtaat tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac
     6541 caccacctgc gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc
     6601 cccgaccacc agcacctgct cgctgggtgc aatagctgca cgacgcaccg catgagcgct
     6661 aatagcgaaa ggttcaatca atgctgccgc ctgcgggtca ataccgtctg cgggcaaaat
     6721 gttcgccacc ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac
     6781 tgaaattttt tcacagcaat tggtacgacc gcttttacac gccgggcatt gctgacaaga
     6841 aatatacgga attaccgcaa cttgctgtcc gatattaaaa ttgtgaatgt tttttcctga
     6901 ttcaataatc tccccacata tttcatggcc taaaacgcgt ggataactga aaaatggctg
     6961 attgcccgcc caggcatgaa tatctgtacc gcaaatcccg acagttttaa ttttaattaa
     7021 tgcttcatta tcgcctggca ccggtctttc ccggacttcc catactaaat tctttatttc
     7081 ctgacaaatt aatactttca ttgtggacat gattcaccgc tccttttatt ttatgacttt
     7141 cagatatcgt cgaatttata agagcgcctg aatttaccca taaaatatgc ggatgatcgc
     7201 gcataaaaat gttttaaatc ggcttttaat gccggactgg agggaagaat gagtcgttcg
     7261 cagaatttac gtcatagcgt cataaatcag gtgattgaag atatggcgag gggcaatatt
     7321 ccttctccgc tgccttcaca aagcggactg gcagaaatgt ataacatcag tcgcactacg
     7381 gtacgccaca tactccagca tttaagcgca tgtggcgtgc tcaccctggt ggggaaaaat
     7441 tatgttatcg cccgcaaacc cgaacagaac gatggatttg attgcatcac ggcctcgctg
     7501 aaagagcaaa accgtctttt cgagcaggca tttttcacca tgattaacct gcgccagtta
     7561 cgcgccggag aaagcttttc tgaacttcag ctggcgcgag cggcaagcgt cagtccggta
     7621 gtggtacgag aatatctttt gaaattcggt cgttataatc ttattcaaag cgaaaagcgc
     7681 ggccaatgga gcatgaagca gttcgatcaa tcctacgccg agcagctgtt tgagctacgc
     7741 gaaatgctcg aaacacattc cctgcaacac tttctcaacc ttcccgatga tgaccctcgc
     7801 tggttgcagg cgaaaaccat gctcgaacgt cacaggacat tgcgcgacag cattggcagc
     7861 aactttcgaa tgttttctca attagaccgt gattttcatg ccatgctgtt atccgctgct
     7921 gacaacatct tttttaatca atcacttgaa attatatcgg tgatctttca ttttcactat
     7981 caatgggatg aaagcgatct caaacagcgc aatatcattg ctgttgatga acatatgacc
     8041 attctcagcg ccctgatttg ccgtagcgat ttagatgcaa taacggcgtt gcgaaaccat
     8101 ctggataccg ccaaacaatc aatgattcgt tccatcaggc aacatcaccg ataaattaag
     8161 tacagattaa aaacattgat tcttggttta tatcactatg ttgtattaat gcagtattaa
     8221 tccaggcgat tgttctgccc ataaggaaat acatagggaa taaacgatgc tggaagtatt
     8281 actctcagcc atcatttaat tccctattac tggcagcgat aaaattatcg gctatccatg
     8341 attaacgttt ccagcacatc taattcactg actatttttg ccagtgtttc atcactgact
     8401 tcttgccttg cgcgcatctg gtaaatagcc gcgcgtttgg catttaacgc cgccagatta
     8461 aatgttcttt ccagcttcgc acgctgcata tgcagggaat tatcattagg cgacatatcc
     8521 tcttcactat gtcgcatata accgataact ctggcccccg tttccataat caactcgtta
     8581 tcgaggtttt ctgttctggc gcgcagcaaa cgttgctcgg tcttttgcac actcagtatg
     8641 gccattttag ccataaacag atcggcagct tcctcttctt tttgtcggtt atcgctgaaa
     8701 atccccacat ttttccctag taataacggc agaataataa tgccgcaaac cagcgaaaag
     8761 aggatcacac ctgtagcgag aaataccagc aagtaacgcc cgggaaaagg tgtgccatcc
     8821 ggcagcaata acgggataga aagcaccccc gcaagcgtga tagctccgcg cacccccgca
     8881 aaggtcgcca gcagcatatg acgcgtttta tatccagcaa acagcatcgg atgtttgccg
     8941 ggtcgtttac ggctatacag acgcatcagc caaagccagc ccaggcgaac aaccattaac
     9001 gccaggtaga tccaaatgac atcaaggagc agcgatccgg tgtccaccag tggatcgttt
     9061 tctgcacgga tcagtgattc cgaaataatg cccggcagtt gtaagcctaa aatcagaaac
     9121 accatgccgt taaagacata ttcaagcatc gaccagctac ttttagcttt aattctcagg
     9181 cgaataggcg cgtggtggac tacgccaatg cgactggttg ttaaccccgc ggcgacagcg
     9241 gcaagaatgc cagataagcc aaaatgttcc gcaaccagat aaacaataaa cggcaacaga
     9301 agcatcaata caatctgaat ggaaggttca tcgtgagcat atcggctgat caacagcagc
     9361 cctttaccat ataaccaggt aacagcgatc cccacggcaa taccgcccaa cgcaacgaca
     9421 aaaaacgcaa tggttgcgcc atgaacgctg aattccatcg ttcccatcgt cactgcaaca
     9481 gcaaatttca acgccaccag gccggaagca tcattcatca acgcttcgcc ttcgagcact
     9541 tcctggatgt ttttgctgat acgccctttc ccgacaatcc ccagcaacgc cacggcatcg
     9601 gtaggcgaca acacggcggc taatgcgaaa gcagcgatga gcggcacatt cggtaagagt
     9661 aaatgaatca catagccgac accaaaaata gtgattagta ccaacacaag tgccaggctg
     9721 ataatttccc ggctgtgctt gatcaattct ttaggttgta tttttgaact ttcagcaaac
     9781 aacaggggtg gtataaacaa taagagaaaa agttcaggat tgaattccac atgcagacca
     9841 aatataggct gggccagtac tgcaccagcg gcaatctgga ttaaaggaag tggtatctgg
     9901 acaggagaca gacgtgtaag cacgctggtt aatgatacta ccaacgtaag aattaaaatt
     9961 gttaaaagta tttccatata ataaacaggc ttcctcattc ataaataatg gatattat
//
LOCUS       DADSZD010000159        10006 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.160, whole genome shotgun sequence.
ACCESSION   DADSZD010000159 DADSZD010000000
VERSION     DADSZD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.160"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            147..299
                     /gene="hokA"
                     /locus_tag="J1Z28_003616"
     CDS             147..299
                     /gene="hokA"
                     /locus_tag="J1Z28_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBA7718416.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(487..699)
                     /gene="cspA"
                     /locus_tag="J1Z28_003617"
     CDS             complement(487..699)
                     /gene="cspA"
                     /locus_tag="J1Z28_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBA7718417.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(980..1270)
                     /locus_tag="J1Z28_003618"
     CDS             complement(980..1270)
                     /locus_tag="J1Z28_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBA7718418.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1704..2414
                     /locus_tag="J1Z28_003619"
     CDS             1704..2414
                     /locus_tag="J1Z28_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBA7718419.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2464..3438)
                     /gene="ghrB"
                     /locus_tag="J1Z28_003620"
     CDS             complement(2464..3438)
                     /gene="ghrB"
                     /locus_tag="J1Z28_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBA7718420.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3542..4201)
                     /locus_tag="J1Z28_003621"
     CDS             complement(3542..4201)
                     /locus_tag="J1Z28_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBA7718421.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4354..6687
                     /locus_tag="J1Z28_003622"
     CDS             4354..6687
                     /locus_tag="J1Z28_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin guanine dinucleotide-containing
                     S/N-oxide reductase"
                     /protein_id="HBA7718422.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWEKGGYTRFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6656..7096)
                     /locus_tag="J1Z28_003623"
     CDS             complement(6656..7096)
                     /locus_tag="J1Z28_003623"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7718423.1"
                     /translation="MIREAQRSELPALLELWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7093..7656)
                     /gene="tag"
                     /locus_tag="J1Z28_003624"
     CDS             complement(7093..7656)
                     /gene="tag"
                     /locus_tag="J1Z28_003624"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBA7718424.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            7814..8512
                     /locus_tag="J1Z28_003625"
     CDS             7814..8512
                     /locus_tag="J1Z28_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7718425.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8741..9943
                     /locus_tag="J1Z28_003626"
     CDS             8741..9943
                     /locus_tag="J1Z28_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718426.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2374 a         2407 c         2577 g         2648 t
ORIGIN      
        1 tgcttgagac tgtttgtctc aggtattcac cgaaaggcag acagagaaaa gccccacctg
       61 actataaatc aaagtgaggc ccctctatgc atagacacca taaggttagc ctcttactcg
      121 ttggaaatca acacaggggg ctgggaatgc cgcagaaata tagattactt tctttaatag
      181 tgatttgttt cacgctttta tttttcacct ggatgataag agattcactg tgtgaattgc
      241 atattaaaca ggagagttat gagctggcgg cgtttttagc ctgcaaattg aaagagtaag
      301 cgtcttcggc gggaaattat tcccgccttt cttacggtgt tgcgcattct cattgcaccc
      361 aaatttattc ttcacaaaaa taacactaga ttctattacg cgatcgatta tttatttcct
      421 gaaaaacaaa taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag
      481 cagagattac aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt
      541 gaaggacact ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa
      601 gtgtacgaac acgtctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc
      661 gttgaaccat tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt
      721 aattgtgcct ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc
      781 actacgaggg gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg
      841 gtctgtacgt caaaccgttg atgtgcatta agccacgcat tggtgggtga tgcaacaatt
      901 atttttcata tttatgatta atcggttgga aaacggtcct gttatcagga ccgtaaacag
      961 aaataaagtg gataaaagtc tattccatca actgcttact taatgccggg ttggcttgaa
     1021 tcaaacgcat caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct
     1081 ttaccatggc gactgatacg cccaaaaccc gggcaaaatc atcgattttt aatcctgtcc
     1141 cttttcgtaa ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct
     1201 gcgtttcatc tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt
     1261 tatattccat tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca
     1321 ttaagaatag tcgggaaaaa cagacacagc ggggtgtagt cagggcatac tctgactatg
     1381 attcgattca gccagtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg
     1441 atatatctct taccactccc ggtttgtaaa gatttcgaaa atctaacgtt ctgcgaaggc
     1501 gtaaatctct ggctacaaaa aaggcaacgg caatatcatt ttttaacaat gaaacaacaa
     1561 attaaccttg tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc
     1621 tgcaagcctg tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt
     1681 ttttgggtga aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc
     1741 ctcttgcggc gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc
     1801 agcgtaaagc gtttatcgac ttcctgcaga atacagtgat gcgtagcggt gaacgtctac
     1861 caaccctgac tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc
     1921 tgtatggtta ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggttgtag
     1981 acagcgttaa cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg
     2041 aaatgaacgg ttcgctgggt gtgctggcgc aacagctgca aaatgcgaag ttgcaggccg
     2101 atgctgcgca ctctgcgtta aaacagagtg atgacctgaa accggtcttt gatcaggcat
     2161 tcaccaaagt ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa
     2221 ctttcacgca acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga
     2281 gctttgtcgc aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac
     2341 tgattgcgcc attaccggca caacatcagg cgtttaatca ggcctggact acagctgtta
     2401 ctgcaaccca ataaagagta aaagcccgag cgattatcgg gctttactcc acgcagtcgc
     2461 ggcttagtcc gcgacgtgcg gattcacaca gttcttttca acctttcctt gtaacgcatc
     2521 aatcaaatta tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc
     2581 aatatgcggt actgcgacga cgttggccat tgagagcaac ggcgaatcta cggacagtgg
     2641 ctcttgttcg aagacatcca gcccggcggc gtgaatttcc cctttctgca atgcagcaat
     2701 cagtgcattt tcatcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt
     2761 cattttggcg aattgttctg cgccaaacag atgatgcgtc tcatccgtta acggcaggat
     2821 caggcaaacg aaatctgact cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa
     2881 gcgttcttct gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaaacc
     2941 aaagtgcgca cgttgtgcca gcgccatacc gatccgtccc atcccgacaa tgcccagtgt
     3001 tttatggtga acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc
     3061 ttttacccgt tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag
     3121 cgtatcggcg acggtttctg ttaatacggt tggtgtgtgc atcagcagaa tttttcgggc
     3181 ggtaagcgca tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag
     3241 tttcggcatt ttttccagca atgcggcatc aacattctca tttgaaccca gcaagccttc
     3301 agcttcggca aaaattgctg cattttgttc gacggtttgt gggctgaggt ttgccacctg
     3361 gtgaacggtg aaatgttctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag
     3421 gataacggac ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa
     3481 atatgatgaa ggaaaactga agaatgccag gtgatttctg catcacctga catgaaaggg
     3541 attacagtgg gcttaaggta atttctacac ggcggttttg cgctttacct tctgcggtgc
     3601 tgttgctggc gattgggtta gccgggccaa ggccttgagt acggatgcgg ctggcgtcca
     3661 cgccctgggt gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt
     3721 tcaggtcgtg actccccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat
     3781 actctttcag taccattgcc acgccggtca gggtgttagc gcccgccggt ttcagggttg
     3841 cgctgctgct gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc
     3901 gggttacgct aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca
     3961 tgtaataacc aacgccgccg cccagagctg cgcctgctgc tgcgccaatg agcgcgcctt
     4021 taccgcgatc tttcttcgaa gaagagagcg caccaatacc cgcgccaacg agagagccca
     4081 gacctgcgcc gatagcagat ttacctgctt cgcgttcgcc ggtgtaaggg ttagttgtac
     4141 agccagatac cgccagagcg ccactcacta cggcggcaat aagataaaca cgtttcttca
     4201 ttgttaatcc ttaataacct ttttattctt tgccacgggt tccgtggcgg gagattatgc
     4261 cgcgtgagca tgaagattat tcctgggaat actcggaaat ttgtaagtaa tatttaactg
     4321 ctcaatacat ctaacctttc aggagtcttc ggtttggcca actcatcctc acgatattcc
     4381 gttctgactg ccgcccactg ggggcctatg ctggttgaaa ccgacggcga aaccgtgttt
     4441 agctcgcgtg gcgcgttagc cacaggaatg gaaaactcct tgcagagcgc ggttcgcgac
     4501 caggttcaca gcaatacgcg ggtgcgattt ccaatggtgc gcaaaggctt tcttgcgtca
     4561 ccggaaaatc cgcaaggcat tcgtgggcag gatgaatttg ttcgcgtgag ttgggatgag
     4621 gcgctggagc ttattcacca tcaacataaa cgcattcgtg aggcttatgg tccggcatcg
     4681 atttttgctg gttcctacgg ctggcgttca aacggcgtgc tgcataaggc ctcgacatta
     4741 ttacaacgct atatggcgtt ggcaggcggt tataccgggc atctggggga ttattcgacc
     4801 ggcgcggcac aggcgatcat gccgtatgtc gtgggtggca gcgaagttta tcaacagcaa
     4861 accagttggc cgctggtgct ggaacatagc gatgtcgtgg tgctgtggag tgctaaccca
     4921 ctcaatacgc tgaaaattgc gtggaatgca tccgatgagc aggggctttc ttacttttcc
     4981 gcactgcgtg acagcgggaa aaagctgatc tgcattgatc caatgcgatc ggaaaccatc
     5041 gatttctttg gcgataaaat ggagtgggtg gcaccacaca tgggcaccga tgttgcgttg
     5101 atgctgggga tcgcccatac gctggtggaa aatggttggc acgacgaagc gtttctggcg
     5161 cgttgcacca caggttatgc cgtcttcgcc tcttatttgc tgggcgagag tgacggtata
     5221 gcgaaaaacg ccgaatgggc ggcagagatt tgtggtgttg gcgcagcgaa aatccgcgag
     5281 ctggcggcta ttttccacca aaatacaacc atgctgatgg caggctgggg aatgcagcgc
     5341 caacagtttg gtgagcaaaa acactggatg ctcgtcacgc tggcggcaat gttggggcaa
     5401 atcggcacac ccggcggcgg ttttggtctt tcttaccatt ttgccaatgg tggtaacccc
     5461 acgcgccgcg ctgcggtgct ctcttccatg cagggcagct tgccgggtgg caccgatgcg
     5521 gtagataaaa tccctgtcgc ccgcattgtt gaagcactgg aaaaccctgg tggcgcatat
     5581 caacataacg gtatggaccg acatttcccg gatattcgtt ttatctggtg ggcgggtggt
     5641 gccaacttta ctcatcatca ggataccaat cgcctgatcc gtgcctggca aaaaccggag
     5701 ctggtggtga tctccgaatg cttctggacg gctgcggcga aacacgcgga tatcgttctg
     5761 cctgcgacca cctcttttga gcgtaatgat ctcaccatga ccggtgatta cagtaatcag
     5821 catctggtgc cgatgaagca agtggtgccg ccacgcgatg aagcgcgtaa tgattttgat
     5881 gtttttgccg acttgagtga acgttgggag aagggtggtt atacacgttt tacggaagga
     5941 aaaagtgagc tgcaatggct ggaaacgttt tataacgttg cccggcagcg cggggcaagc
     6001 cagcaggttg aattgccacc atttgctgag ttctggcaag ccaaccagtt aattgagatg
     6061 ccggaaaacc cggacagtga gcggtttatt cgcttcgccg attttcgccg cgatccgcag
     6121 gcgcatccat taaaaaccgc cagcggtaag attgaaatct tctcgcagcg tattgccgat
     6181 tacgcttatc cggattgccc ggggcatcca atgtggctgg cgccggacga atggcagggc
     6241 aatgccgaac cggaacagtt gcaggtactt tctgctcatc cggcgcatcg tctgcacagc
     6301 cagctgaatt acagttctct gcgcgaattg tacgcggtgg caaatcgtga gcctgtcacc
     6361 attcatcctg acgatgccca gacgcgcggc atacaagatg gcgatactgt tcggctgtgg
     6421 aactcacgcg ggcaaattct tgccggcgcg gtcattagcg agggaattaa acctggcgtg
     6481 atttgtattc acgaaggcgc atggccggat ttggatttaa ccgctggcgg catttgtaaa
     6541 aacggcgcag tgaacgtgct gaccaaagat ctccccagct cgcggctggg gaatggctgt
     6601 gcgggtaata cggcgctggc atggctggaa aaatacaacg gtccggaact gacacttaca
     6661 gcgtttgaac caccggccag ctcataatcc aggtgggtag ttgggtttca tcctgccatg
     6721 cgcaatcgac aatgtgaaaa ccctgcgcat ggtagaaatc tatcgccggt tgattttttt
     6781 gataaacctc cagcatcaga tggggatggc gctgctgcac atactgcatc agcgccttac
     6841 caataccgcg tctgacggcc ttcggtgcga caaacattgc tgcgaggaat cggccttcca
     6901 taatgctgac aaaaccgaga agcttaccgt cttcttccca gacccagttt tgcgcgttgg
     6961 caagatacgc atcccgcacc agcggaatgc agtcacgcca gtaactcgat tttataaagg
     7021 gatgccccca ggttgtactt tccagccaca gttcgaggag cgcggggagt tctgaacgtt
     7081 gcgcttcccg aatcatggtt tatttcccgg atggcaacag cagccaacca catgatcatt
     7141 caccagccca catgcctgca taaaggagta acagattgtg gtaccgacaa acttaaaacc
     7201 acgttttttt agtgctttag atagggcgtc ggaggcgggt gtagatgtgg ggatttcgct
     7261 caacgttgtg gcttgtgtca cctgtggttg atgattgaca aacgaccaga caaagtcggc
     7321 aaatggttcg ccgttctgtt ccatttgcag gtacgcccgc gcattaccaa taattgcctg
     7381 aattttccca cgatggcgga taatcccggc gtcctgtacc agtctttcga catcctcttc
     7441 ctgcattgcg gcaactttca ccggatcgaa ctgatgaaag catgcgcgat agttttcgcg
     7501 ttttttgagg acggtgatcc acgataaccc agcctgctgc ccttcaaggc agatcatttc
     7561 gaacagtttt ttactgtcag tttcaggcac gccccattca ttatcatggt aggcaatata
     7621 aagcgggtcc tgactcaccc agccgcaacg ttccatactt tccctcgcta tgtacccgat
     7681 cgtaaaaatt tcagccgctt ttcttgacgc atcggtgaaa aagtcaatat ttaatacagg
     7741 gctactatgc ccgatatcct tcatacaatg acaataatat tgccgaattc ggctcttccc
     7801 tggagtcgtg ttaatgatca taaaaaaaag cggtggtcgc tggcagctaa gcctgcttgc
     7861 gagcgtggta atcagtgcct tttttctcaa cacagcttac gcctggcaac aagaatatat
     7921 cgttgatacg caacccggac attccacaga gcgttacacc tgggatagcg atcatcaacc
     7981 tgattacaac gatattttgt cgcaacgtat tcaaagtagc caaagggcgc tgggactgga
     8041 agtcaatctg gcggaagaaa cccctgtgga tgtgaccagc agtatgagta tgggctggaa
     8101 ttttccttta tatgagcagg ttacaaccgg cccggtcgcg gcattacatt acgatggcac
     8161 aactacctcg atgtataacg agtttggcga cagtactacc acgctggccg atccgttatg
     8221 gcatgccagc gtgagtacct taggctggcg tgttgactcc cggcttggcg atcttcgacc
     8281 ctgggcgcaa atcagctata accagcaatt tggcgagaat atctggaagg cgcaatcagg
     8341 cctgagccgg atgacggcga caaaccagaa cggcaactgg ctggatgtca ccgtaggcgc
     8401 tgatatgttg ctcaatcaaa atattgctgc ctatgctgcg ctatctcagg cagaaaatac
     8461 cactaacaat agcgactatc tgtatacgat gggggttagt gccagatttt aacgttatag
     8521 atacaattga aacccataaa taacaactaa tgtggtgata gtggggacta acttactaaa
     8581 taataaattt ggcgaataat tgtcgagtca ttcattcctg aactaatgca tttcattccg
     8641 ttctgatggc atttcatgcc gtttttcccc aggcataaag cgcacttcgt tatggttgtc
     8701 agcagagatt tttccttttt attactgcag gaatactgcc atgacacctt caaattatca
     8761 gcgtactcgc tggctgacac tcatcggtac tatcattacc cagtttgctc tggggtcggt
     8821 ttatacctgg agtctgttta acggtgcgct ttctgccaag ctgggggctc ctgtaagcca
     8881 ggttgctttc tctttcggct tgttaagtct ggggctggca atttcgtctt ctgttgcggg
     8941 caaattgcag gaacgttttg gtgttaaacg cgtcactatg gcttccggca ttttgctggg
     9001 attaggtttc ttcctgacag cacattccaa caacctgatg atgctgtggt taagcgccgg
     9061 tgtgttggtt ggtctggcgg atggtgcggg ttacctgttg acgctctcta actgcgtgaa
     9121 gtggtttccg gagcgtaagg gacttatctc tgcttttgct atcggttctt atggtctggg
     9181 cagcctgggt tttaaattta tcgacacgca cctgctcgaa acggtgggtc tggaaaaaac
     9241 ctttgttatt tggggcgcga ttgtactggt gatgattgtc tttggcgcaa cgttaatgaa
     9301 agatgcgccg aagcaggaag tgaaaaccag caatggtgtg gtggagaagg actacaccct
     9361 ggcagagtcg atgcgtaaac cgcagtactg gatgttagcg gttatgttcc tgactgcgtg
     9421 catgagtggt ctgtatgtga ttggtgtagc gaaagatatc gctcaaagcc tggcgcatct
     9481 tgatgcaatt tccgcagcca atgctgtgac ggttatttcc atcgccaacc tttctggtcg
     9541 tctggtgctg ggcattctgt ctgacaaaat cgcccgtatc cgtgttatca ccattggtca
     9601 ggtgattgcg ctggtgggta tggcggccct gctgtttgca ccattgaatg cagtgacgtt
     9661 ctttgcagcg attgcctgtg tggcatttaa ctttggtggc acgattacgg tgttcccgtc
     9721 actggtcagt gagttcttcg gcctcaataa cctggcgaaa aactacggcg tcatttacct
     9781 cggtttcggt atcggcagca tttgtggttc gattatcgct tcactgtttg gcggattcta
     9841 tgtgactttc tacgtgattt tcgccctgct gattctgtca ctggcgcttt ctacgaccat
     9901 tcgccagcca gagcagaaaa tgttgcgtga agcacatatt tagaaatatg ttgctgctga
     9961 aataaatacg atgagggaga cttcgaagtc tccctttttt tatggt
//
LOCUS       DADSZD010000160         9928 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.161, whole genome shotgun sequence.
ACCESSION   DADSZD010000160 DADSZD010000000
VERSION     DADSZD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.161"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(188..1426)
                     /gene="mntH"
                     /locus_tag="J1Z28_003627"
     CDS             complement(188..1426)
                     /gene="mntH"
                     /locus_tag="J1Z28_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+) uptake NRAMP transporter MntH"
                     /protein_id="HBA7718427.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            1762..2964
                     /gene="nupC"
                     /locus_tag="J1Z28_003628"
     CDS             1762..2964
                     /gene="nupC"
                     /locus_tag="J1Z28_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBA7718428.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(3013..5202)
                     /locus_tag="J1Z28_003629"
     CDS             complement(3013..5202)
                     /locus_tag="J1Z28_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HBA7718429.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLSFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5409..5484)
                     /locus_tag="J1Z28_003630"
     tRNA            complement(5409..5484)
                     /locus_tag="J1Z28_003630"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5449..5451),aa:Ala,seq:ggc)
     gene            5720..6064
                     /locus_tag="J1Z28_003631"
     CDS             5720..6064
                     /locus_tag="J1Z28_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBA7718430.1"
                     /translation="MTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKARLVHV
                     NEQVREYIRNAERPESQGETPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLLREGII
                     GLLQRLGIRDSK"
     gene            6066..6458
                     /locus_tag="J1Z28_003632"
     CDS             6066..6458
                     /locus_tag="J1Z28_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBA7718431.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6498..7706)
                     /gene="tnpB"
                     /locus_tag="J1Z28_003633"
     CDS             complement(6498..7706)
                     /gene="tnpB"
                     /locus_tag="J1Z28_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HBA7718432.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLRDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGIVKNVTVCQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILNQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQICGYTANADVNGARNILAAGHAVLACGEMVQSCRPLKQ
                     EPTEMIQATA"
     gene            7714..8255
                     /gene="tnpA"
                     /locus_tag="J1Z28_003634"
                     /pseudo
     CDS             7714..8255
                     /gene="tnpA"
                     /locus_tag="J1Z28_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311304.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(8256..9671)
                     /gene="gltX"
                     /locus_tag="J1Z28_003635"
     CDS             complement(8256..9671)
                     /gene="gltX"
                     /locus_tag="J1Z28_003635"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBA7718433.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLNLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
BASE COUNT         2576 a         2564 c         2336 g         2452 t
ORIGIN      
        1 cagcggcaag ttttacttca aatgaatgtt tcataaactg caccttcaga tgccagatta
       61 tgtccaacat ttggggtgca gttcatagcg cgtcttatca ggcctacaaa ccagctcttt
      121 aacgcgtagg gcggataaag catttacgcc gcatccgcca atggtgccaa atgcgacgct
      181 cattcaacta caatcccagt gccgttccta ccagtaacca gatattcagc gccactacca
      241 gcaccacaat cacccagcct gtctgtttta cgcgtttact gttcaccaga tcgcccatca
      301 acttgctgtc actggtgaaa atcaacagtg gaaccagcgc cagggcgata ccaaaactta
      361 acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga atgacgataa
      421 atgacggcag catggtgact gtacgacgca cccacagcgg gatatgaaag cgaatgaagc
      481 cctgcatcac tacctgcccc gccagcgtcc ccaccaccgt tgaagacagt ccggcagcaa
      541 ccaggcttaa tccaaagacc gttgccgcag catggcttaa cagcggttgc agcgtcagat
      601 aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc gccgcagctg
      661 tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc acatcccatt
      721 tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg agcgaagagt
      781 gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcccgctgcc aggaagaccg
      841 cctccgaagt aggtaaactc gggattacca ttcctttacc cagctgcgcc aggttaggct
      901 gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac ccgccaatca
      961 ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa gtcgcgatcc
     1021 ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa ccgatcgccg
     1081 caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga acccaataga
     1141 accacacgac ggggcgcggg tagtgatcgc gaatctgctc cgccagattt ttaccggtgg
     1201 caatccccag tttggccgag aggatttgaa tcagcatcgc catcaggtta gcccaaacga
     1261 caacccacaa taactgatag ccgaagctgg cacccgcctg aatattggtc gcaaagttac
     1321 cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg agcctcatct
     1381 tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg tgcctctaaa
     1441 acatagcctt tgctatgttt catgctacgt taaatgagaa tgattatcaa attcatttaa
     1501 atgggttgtg atgatttctc tgatagacca ggattatgac tacgaaaaga tagatgactc
     1561 gaatgttatg gttatgttta atattagcac atttacataa ccttccgctt ccatacacaa
     1621 catagcagaa atgtatgaca gatcactatt tttgaagctt gtcacaggac gtcattatag
     1681 tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg aaatttaacc
     1741 tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc acttgccgtt
     1801 gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat ccgttatgtt
     1861 attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc cgacgttggt
     1921 ctgggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tgctcggttt tgccaacgaa
     1981 gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt cttcctgaaa
     2041 gtgctgtgcc caatcgtctt tatctctgcg ctgatcggta ttctccagca tattcgcgta
     2101 ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg catgggcaaa
     2161 ctggaatcct ttaacgccgt cagctccctg attctgggtc agtctgaaaa ctttattgcc
     2221 tataaagata tcctcggcaa aatctcccgc aatcgtatgt acaccatggc agcaacggcg
     2281 atgtccaccg tgtcgatgtc catcgttggt gcatatatga ccatgctgga gccgaaatac
     2341 gtcgttgcgg cgctggtact gaacatgttc agcaccttta tcgtgctgtc gctgatcaac
     2401 ccttaccgtg ttgatgccag tgaagaaaac attcagatgt ccaacctgca cgaaggtcag
     2461 agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc cattatcgtt
     2521 gccgcgatgc tgatcggctt tatcgccctg atcgctgcac tgaacgctct gtttgctacc
     2581 gtgactggct ggtttggcta cagcatctcc ttccagggca tcctgggtta catcttctat
     2641 ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg cagtatcatg
     2701 gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa aattgcttcc
     2761 acgctctctc cgcgtgcgga aggcatcatc tctgtgttcc tggtttcctt cgctaacttc
     2821 tcttcaatcg ggattatcgc gggtgcggtt aaaggcctga atgaagagca aggtaacgtg
     2881 gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt gctgtctgcg
     2941 tcaatcgcag cactggtgct gtaagaccat acataaaaaa gccggggtaa ttccccggct
     3001 tttttacgcc tgttaatcag ctaatggctg cgggcgacca atcaaatacc cttgcagata
     3061 ttgcaccccg agcttatgca atagcgcctg ctgctgttgc gtctcgacaa actccgcgac
     3121 cacactcaat gacttcgctt tcgccagatc ggtaattgat ctcacaatca tcgcatccag
     3181 cgtgttcgtg acgatatctt tcacaaagac gccatcaatt ttgatgatat cagcctgcaa
     3241 acgctttaac cgttcgtagt tggcatatcc ggtgccaaag tcatcaatcg caatccggaa
     3301 accaaactta tgcagctgct cgatgttgta catgctgctt tctgcgttag aaaacgcctg
     3361 ctcctcggtg atctcaagaa tgaccgcctg cggggagatg taataacgct taaacagacg
     3421 aataatccgc ccggcaatat tcttttgcag cagcgtgagc ggcattaaat tgactgaaaa
     3481 gcgtggacct tttttgtcgc aagggtgtgt tgccagccac ttcaacaagg attccagcac
     3541 ttgcaaatca aaacgcgcgc taaggttaaa ctgagcaata aggggcagaa acttatccgg
     3601 ggtcataatg ccgccgtcat acttcagtcg cgcgaggatc tcatcataac cttcaccctc
     3661 tttgttgcga attggctggg cgtagagcag caaatcaccc tgatctaacg ccgtgcgaat
     3721 ggtattcagc aatagcacct gtttagtggt ttgcccggaa accatctcct ctctgctatc
     3781 cagcgccagc acatgatgat gtgcgcagga ttgctccgcc agccagctta actgccccaa
     3841 caagggttgc agggtttcct gatttccatc aaaacgtccc caggcagcac catagcccat
     3901 atccagcccg gtattgttcc agtgaatttg ccgactattc aggatgttaa ccatatgctg
     3961 gagtcgccct tccgtttccg gcccgctcag cactaacagc agttcactac ccggcaattg
     4021 atacaacttt tcgttttcct gcatcaacgg cagcagcgta cggtaaattg agcggataca
     4081 gtgaacgcgc atcattaagc cgtaatgacg actcataaac tcaagattat caatgcgcag
     4141 gcagcaaaaa ctcttgcccg cctcttgctc cggcgcttgc tccaacgcac gaaagttggg
     4201 tagaagcgtt aacggatcgg tcagcgcctg caaatgccag cggcggttaa gccattcact
     4261 acgatgataa atacgcacca tatagagcag gcaaacacta aaggaaatca gtactgcgag
     4321 aataaatgcc agcgaatatt cggttagtac cccttgcaaa aagttctggt tgtaattcag
     4381 aaggcaaagc gttgaaacag cccaggtgag atttaaaaac ggatagcgaa gcttaccgac
     4441 accgagggta aagatgataa agaaaacagg taccaggtaa ccggcaataa agtcattttc
     4501 atacggtgtg cacatcagaa gtagcagcac gctaagagct gccagccagc ttaaggtaaa
     4561 tgcgcgtttc tctttgctca acgacggagc gatatcccta cgccacaata tctgtgcaaa
     4621 gtggggactt acaatcatgc gggtgagata gtaaaaaagc atgttgtaaa tcagcactgc
     4681 ggtgaaaagg cttagcaaat caacgaccgt gaaaatggca tccgcatcgc cgaaaaaggt
     4741 agatatcttt agcggaaaat caaagaaatt tcccacaaga tacatgctgc atttgatgcc
     4801 aatcggcgcc accaaaccaa gccagaacag acgttgccag acgtatcggc tggtcaatcc
     4861 ataacgccaa cgcgtcccca attgccagcg taatattgca caggcacaca ggactgcaaa
     4921 cgcctggctg ccgatcatga cggcggtctg taaaaaagag agatgcaggt tccacagatt
     4981 ggtgcaaaac atcccgcata aaatgggaac gacaccacgc cagccaaaga taaacaagat
     5041 tgacagcatt acgcacagtg gcatccacgc cagaaagata taattggaat ggataacagc
     5101 aagtggcgaa attaaacggg atagctgaat aagtaccacg gtgagcgtaa acgctagtgt
     5161 gaatatcttg atatttttta tcaggttatg ctccacaaac atgagtacac tactctcact
     5221 gatcggggtt ttcaggcaca caaataataa gtttattcgc atcgcgaagc aaacaggggc
     5281 cagcctgttg ctgttttttc agtcagtgat gcattcacca cacggcactg ccaggatata
     5341 gactgtatta caaaagcggc aaaaggcaga gacaaaaaac ccccgctttg cagcgagggt
     5401 ttgaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg
     5461 ctctcccagc tgagctatag ccccatcacg taaagcttgt cgagttgacg ggcggcatca
     5521 tatgaattcc gcccgaatgt gtcaacggca aattgccacg tgtagtttca atcgctgaaa
     5581 aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg
     5641 tttcctgtaa agtggtgtta taaaatgaac tactaataga cccacataca ttcagggaat
     5701 tgttatgttc aaggagagga tgacgccaga tgaacttgcc agactgaccg gctatagccg
     5761 ccagaccatt aataaatggg tacgcaagga aggctggaca acgtcaccaa aacctggcgt
     5821 ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgcaa
     5881 tgctgaacgt ccggaaagtc agggagaaac gcctgccctt tctggtgatg cgccgcttga
     5941 agtattgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc
     6001 cttgcttctg cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa
     6061 ataatatgaa aagattacgc aataaaatga ccaccgaaga attggctgaa tgcctcggtg
     6121 tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc
     6181 ctggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgaattta
     6241 ttcagaacac gccagccttc cataacacgc cgatgcttct ggaagccgaa gaaccccttg
     6301 ccgaatacgc accggggatt cgcactcctg cttatcggca aatcatcagc gcaatagaca
     6361 acatgactca cagtgagcag gaaaaagttg cgcaattttt gtcacgcgaa ggaattcgta
     6421 atttccttgc tcgtctcgac atagatgaat cagcttgaca tcctccacgc cctgaatgac
     6481 gaggatccct gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac
     6541 gggcggcatg actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg
     6601 ttacgagcgc cgtttacatc ggcgttcgct gtatatccac atatctggca tctgaattta
     6661 ctttgtgaca ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg
     6721 tacgctggcg gaacagcaag cacctgaccg ccacgccaga gctgcttgta ctcaagctgg
     6781 cggcgcattt cataccagcc ctgattcagt atcgaacggt ttaaacctga ttttgcccgg
     6841 acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc
     6901 aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac tttgtgaagg
     6961 tagtccctgc ggatatttgc gatacgggaa tgcagtcgct gtattttgcg tttctgcttc
     7021 tgccagttat tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccagcgtc
     7081 ttctggtttt tctgaaaact gtttacaggc ccaaagactg tgccatctga cagcgtggcg
     7141 agtttagcca cgccagcatc cagcccgatc attgacgctg aagggtgaac cggagttgat
     7201 acttcacttt ctgtctgaat actgatgtac cacttaccgc aggactggca gacagtgaca
     7261 tttttcacaa tacccgtgac ctgacggctg ttccggtagc gcatccagcc cagtttcggc
     7321 agaaaaatac ggctgttttc ctgatcgagc ttaacgccct gcgggtagcg gaatgcatca
     7381 ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa gtttttgtaa
     7441 gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc tctaagccat
     7501 tgcgtttcag tggcattttt ccactcaacc agccaggaag ccattttccc gtaagggatg
     7561 tatttattac cggcctcatg attctcattc tgacgtgcca gagcacgatt gaaaacgaaa
     7621 cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg tcttaactgg
     7681 aatttaaatg cctgtagtcg tttcacaggt caaattatat tttggtctgt gaaaaaagaa
     7741 accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc
     7801 ccaaataccg acgccagatt tttgaccatg acgcgacaga aaaactacgc acttactatt
     7861 caaatgtatg tgctgatttt gaagctgaac gggttgaaat ggatggcgaa ccagatcact
     7921 tccatttgtt aattaactat cctcccaaac tggcgatatc cagtctggta aacagcctca
     7981 aaggcgtatc gggtaggtga ctgcgacgag atcgaccaga tattgcagtc aggtattact
     8041 acaaaggcat tttgtggagt cctggctatt ttgccagtag ctgcggaggt gcgccaatat
     8101 ccgtcatccg ccaatacatt gaacaacagc aaacacctgg tcaggtggaa aaccgcgcct
     8161 tatatccccg ccctgaagga cggggtttta cggcgcaccg gataaataaa aaacggcagg
     8221 ggtatctctc ctgccgttta tcttttatgc gctaattact gctgattttc gcgttcagca
     8281 ataaaagcca gcgctttgtt gatacgctcg atactgcggg tcttaccgat tgcgtgaacg
     8341 gtaacatcca gcgccggaga ctgccccgca ccggttacgg cgacacgcag cggcatacca
     8401 actttaccca tacccacttc cagctcatcc gccgtcgcct gaatagcgtg atgaacgttt
     8461 tcagcggtcc agtcagtaat cgcagtcagt ttgtcacgaa ccacttccag cggctggcgc
     8521 gctaccggac gcagatgttt tttcgcggcg tcggcatcga actcagcaaa atcttcgtag
     8581 aaataacggc agctctgtgc catctctttc agcgtcttgc agcgttcgcc cagcagtttc
     8641 accagatcag ccagctgcgg gccgttacgg gtatcgatat tttcctgctc aatgtgccac
     8701 tgtaagtgag tagcaacata ctccggcggc aacgcgttaa tgtagtgatg gttcagccac
     8761 agcagcttgt cggtgttgaa cgcactggca gatttgctga cggcattcag agtgaagtat
     8821 ttaatcatct cttcacgagt gaagatttcc tgatcgccgt gggaccagcc cagacgcacc
     8881 agatagttca gcagtgcttc tggcagataa ccatcatcac gatactgcat tacgctgacc
     8941 gccccgtgac gtttggacag ttttttaccg tcatcgccgt tgatcataga aacgtgcgcg
     9001 taaaccggta ccggcgcttt cagagcctta agaatgttaa tctggcgcgg tgtgttgttg
     9061 atatggtctt cgccacggat aacgtgggtg atttccatat cccagtcatc gacaaccaca
     9121 cagaagttat aggttgggga accatcggta cggcggataa tcagatcatc cagctcctgg
     9181 ttgctgaact cgatcggacc acggatctgg tcgtcaaaaa caacagaacc ttcctgcggg
     9241 ttagcaaaac gtacgacaca cggctcatca tcagcgtgat gctcatgact gtggcggcag
     9301 cgaccgtcat aacgcggctt ctcacctttc gccatttgct cttcgcgcag cgcttccagg
     9361 cgctctttag agcaatagca tttataagct gtgccctctt ccagcatctg atcgatcacc
     9421 gcgttgtagc gatcaaaacg tttggtctgg tagtacggac cttcatccca ctccaggttc
     9481 agccagttca tgccatccat aatggcttcg atagcttccg gcgtggaacg ctcaagatcg
     9541 gtgtcttcaa tacgcagcac gaactcaccg ccgtggttac gtgcaaaaag ccaggagtaa
     9601 agagcagtac gcgcgccgcc aacgtgcaga tagcctgttg ggcttggcgc gaagcgagtt
     9661 ttgattttca tgaaatggcc ttacgtttag aaagatgccg acaaccggca aatcctggaa
     9721 aaattgagtg ggcgatattc tatcactccc gcctgattcc tcaatgtagt tcgggttgca
     9781 accttacgca ttattgtttt cgttgacaaa ttgcgcaaca cggtctgttt tgcgatcatc
     9841 ttgcttaaat ttacgacgaa cgaacaattt ctttagaaaa tgcgttgact cattttgaac
     9901 tctccctata atgcgactcc acacagcg
//
LOCUS       DADSZD010000161         9764 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.162, whole genome shotgun sequence.
ACCESSION   DADSZD010000161 DADSZD010000000
VERSION     DADSZD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.162"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(113..2686)
                     /gene="clpB"
                     /locus_tag="J1Z28_003636"
     CDS             complement(113..2686)
                     /gene="clpB"
                     /locus_tag="J1Z28_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBA7718434.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMIRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLDVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2816..3547)
                     /gene="pgeF"
                     /locus_tag="J1Z28_003637"
     CDS             complement(2816..3547)
                     /gene="pgeF"
                     /locus_tag="J1Z28_003637"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBA7718435.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLIGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKSSTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="J1Z28_003638"
     CDS             complement(3544..4524)
                     /gene="rluD"
                     /locus_tag="J1Z28_003638"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBA7718436.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4659..5396
                     /gene="bamD"
                     /locus_tag="J1Z28_003639"
     CDS             4659..5396
                     /gene="bamD"
                     /locus_tag="J1Z28_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBA7718437.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5666..6007
                     /gene="raiA"
                     /locus_tag="J1Z28_003640"
     CDS             5666..6007
                     /gene="raiA"
                     /locus_tag="J1Z28_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBA7718438.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     gene            6111..6158
                     /locus_tag="J1Z28_003641"
     CDS             6111..6158
                     /locus_tag="J1Z28_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718439.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6257..7417
                     /gene="pheA"
                     /locus_tag="J1Z28_003642"
     CDS             6257..7417
                     /gene="pheA"
                     /locus_tag="J1Z28_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBA7718440.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAVMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7460..8581)
                     /gene="tyrA"
                     /locus_tag="J1Z28_003643"
     CDS             complement(7460..8581)
                     /gene="tyrA"
                     /locus_tag="J1Z28_003643"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBA7718441.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8592..9662)
                     /gene="aroF"
                     /locus_tag="J1Z28_003644"
     CDS             complement(8592..9662)
                     /gene="aroF"
                     /locus_tag="J1Z28_003644"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBA7718442.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
BASE COUNT         2493 a         2603 c         2284 g         2384 t
ORIGIN      
        1 aaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaacatagtc
       61 ttttccgtct aatttataga caaaaacgag ccccgaaggg ctcgttttat cattactgga
      121 cggcgacaat ccggtcttca ttaacttcca ggcgaataac tttacccgga accaattcac
      181 cagacagtat ttgctgtgcc agcgggtttt cgatctgctg ctggatagca cgcttcagag
      241 gacgtgcacc atagaccgga tcgtaaccgt tctcgctcag cagtttcagc gcctcgtcag
      301 aaatgtggat ttcataacca cgttcttcca gacgtttgta cagacgtttc aactgaatct
      361 gcgcaatcga cgcaatgtgt tgttcaccca gcggatggaa gacgacgact tcatcgatac
      421 ggttaatgaa ttccggacgg aagttatggc ttaccacatc gagcaccagc tctttcatgt
      481 gcgcataatc cagttcacca aaacgttcct gaatcagatc ggaaccgagg ttagaggtca
      541 taatgacgac cgtattacgg aagtcgaccg ttctcccctg cccgtcagtc agacgcccat
      601 catccagtac ctgcaacaga atgttgaaga catccggatg cgctttttcc acttcatcca
      661 gcaggatgac ggaatacgga cgacgacgca ccgcttcggt caggtagcca ccttcttcat
      721 aaccgacata tcccggaggc gcaccaacca gacgagacac cgagtgtttc tccataaact
      781 cggacatatc gatacggatc atcgcctcgt cactatcaaa cataaagttc gccagcgcct
      841 tacaaagctc agttttcccc acacccgttg ggccgaggaa caggaatgaa ccaatcgggc
      901 gatttggatc cgccagcccc gcacggctac gacgaatagc gttagatacc gcatcaaccg
      961 cttcgttctg accaataacg cgatggtgca gttcttgctc catacgcagc agtttttcgc
     1021 gctcgctttc catcatgcga gaaaccggaa tccccgtcca acgcgccagc acttcagcaa
     1081 tttcggcatc ggtcacttta ttacgcaaca gacgcatagt tttgccttcg agctgcgttg
     1141 cggcttccag ttgcttttcc agttccggga ttttgccgta ttgcagttca gacatccgcg
     1201 ccaggtcccc cacacggcga gcctgttcaa tagcgatttt cgcttgttcc agttccgctt
     1261 taatggtctg cgtaccagaa agcgatgctt tctctgcttt ccactcttct tctaactcgg
     1321 agtactgacg ttctttgtcg ctcagttctt cgttgagcat atccaggcgt tttttactgg
     1381 cttcatcaga ctctttcatt aacgcctgtt gttccagttt gagctggatg atacgacgat
     1441 cgagtcggtc gagttcttct ggttttgagt caatctgcat acgaatgctg gatgctgctt
     1501 catcgatcag atcgatggct ttatccggca gctgacggtc agcaatgtag cgatgagaca
     1561 acgtcgccgc tgcaacaatt gccgggtcag taatttgcac atggtggtgc agctcgtaac
     1621 gttctttcag gccacgcaga atagcaatgg tgtcttcaac agaaggctcg gcaacaaaca
     1681 ctttctggaa acgacgttcc agcgcagcat ctttttcgat gtactggcga tattcgtcaa
     1741 gcgtcgtggc acctacgcag tgcaattcac cacgcgccag cgccggtttc agcatgtttc
     1801 cggcgtccat tgcgccatcg gctttacccg cgccgaccat ggtatgtaat tcgtcgataa
     1861 acaggatgac gttgccttcc tgtttagcaa gatcgttaag tacgcctttt aaacgttctt
     1921 caaactcacc gcgatatttc gccccagcca ccagcgcgcc catatccagc gccagtacgc
     1981 ggcggccttt caacccttcg ggcacttcgc cgttgataat acgctgcgcc agaccttcaa
     2041 cgatggcagt tttaccgacg ccgggttcac caatcaatac cgggttattt ttagtacgac
     2101 gttgcagcac ctgaatggta cggcgaattt cttcatcacg accaatcacc ggatcgagtt
     2161 tgccttgttc ggctcgttcg gtaaggtcga tggtatattt tttcaaagcc tgacgttggt
     2221 cttcagcacc ttgatcgttc acgctttcac ctccacgcat ttgttcaatc gcttgagtaa
     2281 tgttggcggt ggtcgcccct gctgctttca ggatgtcggc cagcgtgccg cgagactcaa
     2341 gtgccgccag aacgaacagt tctgacgaga taaagttatc accacgtttt tgcgccagct
     2401 tgtcgcaaag attaagaacg cgcaccagat cctgtgatgg ctggacatca ccaccagtac
     2461 cttcaacctg cggtaaacga tttaatgcct gattgatatc tgtgcgcaac tggccagcat
     2521 ttatgccagc ggatgttaat aaaggactga ccgaaccccc ttcctgattc agcagggcgc
     2581 tcattaaatg aagtggttcg atgaattggt tgtcgtgccc gagtgcaagt gattgggcat
     2641 cggcaagagc aagctggaat ttattagtaa gacgatccag acgcataact cctcccataa
     2701 cggatcaaag ttgctactgg agattaaatg aggtcatccc tcaattattc aaggttattg
     2761 accagattaa tgtgaaaaga aaatcacgcg taccggatcg tcttgattct ttaggttata
     2821 tcagccaaat gaaacttgcc atacgaccgg tagtcttgtc gcgacgataa gagaagaaag
     2881 tctcattttc cgtatatgta caacggtcac cgccaaaaat ttgttcaaca ccaacgctcg
     2941 ccagacgctg ccgggcaagc tgataaatat ccgccagata cttatcacca tgctgaatga
     3001 aagctgtact cgatttggcg tctactgcca taaacgcctc gcgaacctcc gcccccactt
     3061 cgaacgcacg tggcccaatt gccggcccta accaggcgag aatattttcc ggattatcag
     3121 caaaacagga aaccgtctct tccaacacgc ctgcgcacag tccacgccag ccagcatgag
     3181 cggcggcgac ctccgttccc gcacgattgc aaaacagcac gggcaggcag tcggcagtca
     3241 tcacagcgca aaccgtgcca ggcgtattgc tataagaggc atctgctcgt tttgaggcat
     3301 aaggttcgcc aataagctta agcacgtctt tgccgtgtac ctgctcaagc cagaccggtt
     3361 tagaaggcaa attgcccgca gcaaaaagtc gcttgcgatt ctcctcaacg tgatccgggt
     3421 tatcaccaca atgagcgcca aggttgagag agtcgtaagg cggcaagctc acgccgccga
     3481 tacgggtaga gctacaggcc gcaacacctt ttggcagcgg ccactgcggg acaatcagct
     3541 tactcataac cagtccactt catccttatg ctcttcgaaa tcggcgcgca tcacctcaat
     3601 cagctccacc atatcttgtg gaataggcgc atgccattcc atttcgatac cggagatcgg
     3661 gtgataaaga cgcagcatgg ttgcatgaag cgcctggcgg tcaaacttac gcagcgtgga
     3721 gataaacgct tccgaagcac cttttggcgg acgcggacgg ccaccataaa ccggatcacc
     3781 caccagcgga tgagtgatat gggccatatg cacgcggatc tggtgcgtac gtccagtttc
     3841 cagacgcaac cgcagacgcg tgtgcacacg gaagtgttcc atgatgcgat agtgagtgac
     3901 cgccggttta cccatcggat gcaccgccat gtgagtacgt ttggtcgggt ggcggctgat
     3961 tggttcatct actgtaccgc cagcggtcat atgaccaatt gccaccgctt catactcacg
     4021 agtaatttca cgccgttgca aagattcgac taaacgcgtc tgagccggaa cggtttttgc
     4081 cacaaccatc aggccggtgg tgtctttatc cagacgatgg acgatgcccg cacgcggtac
     4141 atcggcaatg ggcggatagt aatgaagcaa cgcattcagt accgtaccat ccgggttacc
     4201 cgcgccagga tgtaccacca ggtcgcgtgg tttattaatg acgatgatat cttcatcttc
     4261 atagacgata tccagcggga tatcctgcgg ttcaaaacgc gcttcttctt caatctcagc
     4321 gttgatggcc acctgctcgc cacccaatac tttttctttc ggcttatcac acactttgcc
     4381 gttaaccagc acgcgctggt cgaggatcca ttcttttatt cgcgaacgtg aataatccgg
     4441 gaacatttcg gccaaagcct gatctaagcg ttgaccgagt tggttttcgg acaccgttgc
     4501 agtgagctgt actcgttgtg ccatatatac tgcttcttcg tttatcgttg gggttttacg
     4561 gctttgccgt ttaatatagt gtgctattgt agctggtctt aaccgggagc aggaacagag
     4621 aatctcccgt attacatttt gaggaaagtc aaaacgtcat gacgcgcatg aaatatctgg
     4681 tggcagccgc cacactaagc ctgtttttgg cgggttgctc ggggtcaaag gaagaagtac
     4741 ctgataatcc gccaaatgaa atttacgcga ctgcacaaca aaagctgcag gacggtaact
     4801 ggagacaggc aataacgcaa ctggaagcgt tagataatcg ctatccgttt ggtccgtatt
     4861 cgcagcaggt gcagctggat ctcatctacg cctactataa aaacgccgat ttgccgttag
     4921 cacaggctgc catcgatcgt tttattcgcc ttaacccgac ccatccgaat atcgattatg
     4981 tcatgtacat gcgtggcctg accaatatgg cgctggatga cagtgcgctg caagggttct
     5041 ttggcgttga ccgtagcgat cgcgatcctc aacatgcacg agctgcgttt agtgactttt
     5101 ccaaactggt gcgcggctat ccgaacagtc agtacaccac cgatgccacc aaacgtctgg
     5161 tattcctgaa agatcgtctg gcgaaatatg aatactccgt ggccgagtat tatacagaac
     5221 gtggtgcatg ggttgctgtc gttaaccgcg tagaaggcat gttgcgcgac tatccggata
     5281 cccaggctac gcgtgatgcg ctgccgctga tggaaaatgc ataccgtcag atgcagatga
     5341 atgcgcaagc tgaaaaagta gcgaaaatca tcgccgcaaa cagcagcaat acataacaga
     5401 aacctgaaac acaaaacggc agccttgagc tgccgttttt ttattctgtt agttgtgaaa
     5461 ctgaagcgat ttagtcacta tcgatctcat caaatatggc tcgctttgag atattcctca
     5521 agtaaaaaaa cacctcttcc tgcgatttct cacaaaaaag attcgttgac aaaaagtgac
     5581 aaaattatga gatttccatc acacattttg acatcaggaa cgttatgctg aattcaccaa
     5641 gacgggaaga caagaggtaa aatttatgac aatgaacatt accagcaaac aaatggaaat
     5701 tactccggcc atccgccaac atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac
     5761 acatctgatt aatccacata tcattctgtc caaagagcca caagggtttg ttgctgacgc
     5821 cacaatcaat acacctaacg gcgttctggt tgccagtggt aaacatgaag atatgtacac
     5881 cgcaattaac gaattgatca acaagctgga acggcagctc aataaactgc agcacaaagg
     5941 cgaagcacgt cgtgccgcaa catcggtgaa agacgccaac ttcgtcgaag aagttgaaga
     6001 agagtagtcc tttatattga gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac
     6061 agcgtgaaaa cagtacgggt actgtactaa agtcacttaa ggaaacaaac atgaaacaca
     6121 caccgttttt cttcgcattc ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg
     6181 aaacagaatg cgaagacgaa caataaggcc tcccaaatcg gggggccttt tttattgata
     6241 acaaaaaggc aacactatga catcggaaaa cccgttactg gcgctgcgag agaaaatcag
     6301 cgcgctggat gaaaaattat tagcattact ggcagagcga cgcgaactgg ccgtcgaggt
     6361 gggaaaagcc aaactgctct cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt
     6421 gctggaaaga ttaattacgc tcggtaaagc gcaccatctg gacgcccatt acattactcg
     6481 cctgttccag ctcatcattg aagattccgt attaactcag caggctttgc tccagcaaca
     6541 tctcaataaa attaatccgc actcagcacg catcgctttt ctcggcccca aaggttctta
     6601 ttcccatctt gcggcgcgcc agtatgctgc tcgtcacttt gagcaattca ttgaaagtgg
     6661 ctgcgccaaa tttgccgata tttttaatca ggtggaaacc ggccaggccg actatgccgt
     6721 cgtaccgatt gaaaatacca gctccggtgc cataaacgac gtttacgatc tgctgcaaca
     6781 taccagtttg tcgattgttg gcgagatgac gttaactatc gatcattgtt tgttggtctc
     6841 cggcactact gatttatcca ccatcaatac ggtctacagc catccgcagc cattccagca
     6901 atgcagcaaa ttccttaatc gttatccgca ctggaagatt gaatataccg aaagtacgtc
     6961 tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga
     7021 agctggcggc actttgtacg gtttgcaggt actggagcgt attgaagcga atcagcgaca
     7081 aaacttcacc cgatttgtgg tgttggcacg taaagccatt aacgtttctg accaggttcc
     7141 ggcgaaaacg acgttgttaa tggcgaccgg acaacaagct ggtgcactgg ttgaagcgtt
     7201 gctggtactg cgcaaccaca gtctgattat gacccgtctg gaatcacgtc cgattcacgg
     7261 taatccgtgg gaagagatgt tttatctgga tattcaggcc aatcttgaat cagcggtaat
     7321 gcaaaaagca ttgaaagagt taggggaaat cacccgttcg atgaaggtat tgggctgtta
     7381 cccaagtgag aacgtagtgc ctgttgatcc aacctgatga aaaagtgccg gatgatgtga
     7441 atcatccggc actggattat tactggcgat tgtcattcgc ctgacgcaat aacacgcggc
     7501 tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt
     7561 caataaacgc ctgcttatcg ccctgctcca gcaactcaat cgcctcgccg aaacgcttat
     7621 agtaacgttt gattaacgcc agattacgct ctgacgacat aataatgtcg gcataaagct
     7681 gcggatcctg agcaaacagt cgcccgacca tcgccagctc aaggcggtaa atcggcgaag
     7741 agagcgccag tagttgctca agctgaacat tttcttctgc caggtgcagc ccataagcaa
     7801 aagtagcaaa gtggcgcaga gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc
     7861 taatacgatg caaccgagcg ccccagacct gaatttgctc cagaaaccat tggtatgctt
     7921 ccggcttacg tccatcacac cagaccacaa cttgctttgc caggctaccg ctgtccgggc
     7981 cgaacatcgg gtgtaacccc agcaccgggc catcgtgcgc cgccagcatg gcctgtaatg
     8041 gtccattttt caccgatgcc agatcaacca gaatacaatc tttcggtaaa ggcggtaatt
     8101 tgccgataac ttgctcagta acgtggatgg gcacactaac aatcaccatt ccggcatcgg
     8161 caacaatatc agccgctcga tcccagtcat gttgctccag aatccgcacc tgataacccg
     8221 agagtgtcag catcttctcg aacagacgtc ccatctgacc gccaccaccg acgataacca
     8281 ccgggcgcag tgacggacaa agcgttttaa atcctttgtc gttttcactg gagtaagatt
     8341 cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg cggtacaccc agagcttccg
     8401 cctctgcacg acgcgaggcc aacatagatg cctcgcgctc cggaacataa ataggcagtc
     8461 caaagcggct tttcacctcg cccacttcag caaccagttc cagacgcttc gctaataaat
     8521 tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca
     8581 taataaacct cttaagccac gcgagccgtc agctgcccgt tcagatcctg atgaatttca
     8641 cgcagcaagg catcggtcat ttcccagcta atgcaggcat cggttacgga tacaccgtat
     8701 ttcatttcac tgcgcggttg ctcggaagac tgattgccct cgtggatatt actttcgatc
     8761 atcagaccaa taattgagcg attgccatct ttgatttgag caaccacgga ttctgccacc
     8821 gcaggctgac ggcgataatc tttattggaa ttaccgtggc tgcaatctac catcagagac
     8881 gggcgcagtc ccgcctgttc catctctttt tcacattgcg caacatccgc agggctatag
     8941 tttggcgctt taccaccgcg caggatcaca tgcccgtccg gattcccctg agtttgtagc
     9001 aacgcaacct gccctgcctg gttaatgcca acaaaacggt gcggctgggc ggcggcacgc
     9061 atagcgttaa ttgccgttgc cagactgcca tcggtgccgt ttttaaaacc aaccggcatg
     9121 gaaagcccgg aggccatttc acggtgagtt tgcgattccg ttgtacgagc accaattgct
     9181 gaccagctaa acagatcgcc caggtattgc gggctattcg gatctaacgc ttccgtcgcc
     9241 agtggcagtc ccatattcac cagctcaagc agcaatttac gcgcgatctg cagcccggct
     9301 tctacatcaa aagagccatc catatgggga tcgttaatta accctttcca gccgacagtg
     9361 gtacggggtt tttcaaaata gacgcgcatt accagataga ggctatcgct gacctctgcg
     9421 gcaagggctt taaatcgacg agcatattcc agagcagttt ccggatcatg aatggaacaa
     9481 ggaccacata ctaccagcag acgaggatcg cgcccggcga taatatctga aatggtttta
     9541 cgcgagtcag caatctgggc ttcttgttgc aggcttaatg gaaaagcggc cttcagttgt
     9601 tccggagtca ttaaaacctg ttcgtcggta atatgtacgt tattcagcgc gtctttctgc
     9661 atgatggcga tcctgtttat gctcgtttgc gatagttgat cctcagcgag gatgacgtaa
     9721 cgataacaca taaagtaaag ttttcaatcc atatttcgta catg
//
LOCUS       DADSZD010000162         9716 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.163, whole genome shotgun sequence.
ACCESSION   DADSZD010000162 DADSZD010000000
VERSION     DADSZD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.163"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(106..1761)
                     /gene="treC"
                     /locus_tag="J1Z28_003645"
     CDS             complement(106..1761)
                     /gene="treC"
                     /locus_tag="J1Z28_003645"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBA7718443.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLYK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRRFYIWRDGEPETPPNNWRSKFGGSAWRWHVESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(1811..3232)
                     /gene="treB"
                     /locus_tag="J1Z28_003646"
     CDS             complement(1811..3232)
                     /gene="treB"
                     /locus_tag="J1Z28_003646"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBA7718444.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GIFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(3351..4298)
                     /gene="treR"
                     /locus_tag="J1Z28_003647"
     CDS             complement(3351..4298)
                     /gene="treR"
                     /locus_tag="J1Z28_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBA7718445.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKTHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            4483..4536
                     /gene="mgtL"
                     /locus_tag="J1Z28_003648"
     CDS             4483..4536
                     /gene="mgtL"
                     /locus_tag="J1Z28_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBA7718446.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            4677..7373
                     /gene="mgtA"
                     /locus_tag="J1Z28_003649"
     CDS             4677..7373
                     /gene="mgtA"
                     /locus_tag="J1Z28_003649"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBA7718447.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7579..7965)
                     /gene="ridA"
                     /locus_tag="J1Z28_003650"
     CDS             complement(7579..7965)
                     /gene="ridA"
                     /locus_tag="J1Z28_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBA7718448.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(8038..8499)
                     /gene="pyrI"
                     /locus_tag="J1Z28_003651"
     CDS             complement(8038..8499)
                     /gene="pyrI"
                     /locus_tag="J1Z28_003651"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBA7718449.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(8512..9447)
                     /gene="pyrB"
                     /locus_tag="J1Z28_003652"
     CDS             complement(8512..9447)
                     /gene="pyrB"
                     /locus_tag="J1Z28_003652"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBA7718450.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(9451..9585)
                     /gene="pyrL"
                     /locus_tag="J1Z28_003653"
     CDS             complement(9451..9585)
                     /gene="pyrL"
                     /locus_tag="J1Z28_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBA7718451.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
BASE COUNT         2309 a         2453 c         2568 g         2386 t
ORIGIN      
        1 atatgtaaat tttttaatga attttttgat tataaaatca acaaaaacaa cctactgaca
       61 ggcaaaaccc cggaatgaca ggcattaccg gggcttagcg aagatttact tctgtaacca
      121 ccagacagcc tcaaaaggcc gtaaagtcat ggcacagggt tgtggtgagg cttcttcgta
      181 gttatgcatc acaagctgcc agttgccgag cattttccct ggctgccagg gttgggtctc
      241 acggctaagg ttggcaatga ccagcaaggt ttgccccttc cactcacggc gatagcacca
      301 caatacaggg ctgtttggca gcagatcctg gtaatcgccc catgtcagga cggcttcttg
      361 cttacgcagt gcgattaact tttggtaggt gtaaaacacc gaggactcat cggccagcgc
      421 tgcttctacg ttgatttcct gatagttatc gcccaggcca atccacggtt cgccagccgt
      481 aaacccggca ttatcgccgt tggtccattg catgggtgtg cgactgttgt cacgggattt
      541 actggcgagg attgccaata actcgtcggc atcacgccca tcgttgcgca gctcggcaaa
      601 catattgagg ctctccacgt cgcgatagtc agtaatgcgc gtgaaatgtg ggttggtcat
      661 gccaatctct tcgccctggt agatatacgg cgttccctgc atgccatgca gcaccatcgc
      721 cagcattttt gccgcaggca cgcggtattc accttcatca ccaaagcgag aaacaatgcg
      781 cggctgatcg tggttacacc agaacaaggc attccatgcc acgttgtgca ttccttgttg
      841 ccagtggcgg aacaatgttt tcaacgccac aaagtcaggt ttagccagcg tccatttttc
      901 accaccggga taatcgacct tcaggtgatg aaaattaaag gtcatcgaca attcactgcc
      961 tgtcagtgcc gcgtatcgct ggcaatgctc aaggctggtg gaggacattt cacctacggt
     1021 cattaacccg cgtggcgtaa acacatcgcg gttcatctcg tgcaaaaact cgtgtgctcg
     1081 tggcccgtcg gtatagaagc gacgcccatc gccatccagg tcttcagggt aacgcgggtc
     1141 tttggagatc agattcacca catccaggcg caacccgtcg accccacgat cggcccagaa
     1201 ctcacagact tttttcagct ctgcgcgtac cgctggattc tcccagttga gatccgcctg
     1261 ttctggtgca aagagatgca aatagtactg ttcgctttct acatgccagc gccacgcact
     1321 accaccgaat tttgaacgcc agttgttcgg tggcgtttct ggttctccat cgcgccagat
     1381 ataaaaccgg cggtaagggc tttctttgtt cagcgcctcg cgaaaccagg catgttgggt
     1441 agaggtatgg ttaaacacca tatcgagaat gatacgaatc ccacgcgatt ttgcctgtgt
     1501 caccagttcg tcaaaatcgt ccagcgtgcc ataggtggga tcaatcgccg tataattcgc
     1561 tacgtcgtaa ccgttatcga cctggggaga gacataaaag ggggttagcc agatggcatc
     1621 aacgcccagt ttatacagat agtccaggcg ttggataacg ccacgtaaat cgccggtgcc
     1681 gctacccgtg gtgtcctgaa aactctttgg ataaatctgg tagataacgc cgttttgcca
     1741 ccagtgggga agatgagtca ttacgttatt cctgcaaatg cgagggggcg caattgcgcc
     1801 ccgaagaaaa ttaaacaatg tccagcgtgc ccaggcggta tttccgctga tagataaacg
     1861 aggtgagtac aatcgggatg atgatggcga tagccattgc cagcgcaaac acctgccagt
     1921 agctcggctg aatcgagaga attcccggca ggccgcctac gccgatgcca ttcgccataa
     1981 cgccgttcag gccgcatagc aatcctgcca gaccagaacc aatcatcgcg cacagcatcg
     2041 ggaagcgata tttcaggttg atgccgtaca tagcaggctc agtgactcca agccaggcgg
     2101 agatagcggc aggcacggag atctcgcgtt cattgtgctt gcggctggaa atgataatac
     2161 ctatcacggc ggagccctga gcaatattcg acagcgcaat cagcggccac actggcgtgc
     2221 cacccatgct ttgaatcatc tgcaaatcaa tagcaagcgt ggtctggtgt acgccggtga
     2281 tcaccagcgg ggcgtacagg aagccaaaca atgccgcgcc aatcggagca aagctgccgg
     2341 tcatcaggtg acgtaccgca aaggcaacgc catcgccaat catgcgacca aacggaccaa
     2401 tcagcgcatg ggcgaggaac accgcgagga tcagcgaaca gacgggcacc accaccagat
     2461 agaggtaatc cggcacgatg cgcttaagac gagtttcaat aacgcccagc gccagtccgg
     2521 ctaacagtgc cgggatgacc tgcgcctgat agcccacttt ggcgatgctg aatatgccaa
     2581 agtcccatac ttccggcagc tgctgcccga gcagataagc gttcatcagc tgtggagaaa
     2641 ccagcgttac gccaagcacg ataccgagga tcggtgtacc gcccattttt ttcaccgctg
     2701 accagcaaat cccgaccggc aggtagaaga agatcgcttc accgatcaac cacagaaaat
     2761 cgtagatcgt ttgcagggaa gggtacattt gcgccagcgt ctgaccgttg ctcatgggca
     2821 aatcgccgat cacattgcga aaaccgagga tcaaaccacc actaatcaac gcgggcagca
     2881 acgggaagaa gatctccgcg aaatgagaga tcaactgctc atgccatttc atattctgcc
     2941 gggcggcttt ttttacctgc tctttatcaa cctgcgcctg tccggttgac gcaatcagtg
     3001 cttgatagta atcacccacg ttggtgccaa tcaccacctg aaattgcccg gcattggtga
     3061 aacagccttt caccatgggg agttgctcaa tttctttcgg tctggcattg gccggttggt
     3121 tgaggacaaa gcgcaggcga gtaatacagt ggctcaccgt cgcaatattg ccgcgcccgc
     3181 cgaccagttc aatcaaccga tcgatatccg tttggtttat tttgctcatc ataaagcccc
     3241 atggcagatg acatttttgg ttggctgcag aatatattgc gcggaaaaat ttaaaaacgg
     3301 gaacgttccc gaaacgcagc gaagatcaca atttatcgtt caggaaacga tcaggacagg
     3361 gtggcgggga tgatgatttg ttgcggttcg ctgcgcccgg ttacctgcgc gatcaactgg
     3421 caggccgcct ggcgtccagc ttcggcataa ccgggatcta cggtgacgat ctccggatgg
     3481 aggaatttca ttaacggtgt attaccgacg ctcgccagtt gcaaggtgtc gatgcgttgc
     3541 tcttgcaggt atttacttgc gccaagtgcc agcgtgtcgg ttgcgcacag taacgcggta
     3601 gtttcaggcg taatcacttt tgctacgttc tcatagcctt gcttcatagc aagccctggc
     3661 agagcggcaa cgggatgcaa tttatgcgtt ttgcagaacg caaggtaggc ttcgtgacgt
     3721 cgcttaccgg tcgtcacgtc actatgcggc acgccgagat agctgatatt acgatgtccc
     3781 tggtcataca gccgttgcat caggatcttg attgcccctt cgtcgtcata acagaccgaa
     3841 gcaaagcctt ttgcgtcacg cgccagcaga accagcgatg actgccagtg ggctaacatt
     3901 tcttctgtta tgccggtaaa accgaacagc actacgccgt cgatattacg ccgtttcagc
     3961 acccccaaat gttcggcaac taattgtggg gaaaactgac tttccatcat gattggatcg
     4021 taaccttgtt catagaacgc tggcagcatg gtttgaacgg cgagattttc tgacaacgaa
     4081 tccagacggg taacaatgat ggcgaccact ttatcgcttt gcccacgcat agcgcgcgcg
     4141 gagcgggaag gggaaaatcc atgctgattc atcactgctt caacacgctc gcgcgtgcgc
     4201 tggctcacgc cgctttcgtt attcagcacc cgggaaactg tagatttccc cacgccgctt
     4261 aagcgcgcga tgtctttgat ggtcagccga ttttgcatcc tgttgtccca taacgtgttg
     4321 tttaattatt tgagcctaac tttacctgtg cattcagcaa tgggtaaagt ctggtttatc
     4381 gttggtttag ttgccagcag gtattatatc gccataaatg ctacgaatat tattggattc
     4441 tccttattat ttgcggcgct tttttcactt accggaggtt atatggaacc tgatcccacg
     4501 cctctccctc gacggagatt aaaacttttc cggtaagccc gtcttttcac ggcgttaccg
     4561 gatgcgtaag gccgtgacgt tttaacgtcc ctgctcagct ttattacctt caggtaaggc
     4621 ttcgccacgc ctgaagacat ttctgtactg tttcagacag tgcggaggga ctccttatgt
     4681 ttaaagaaat ttttacccgg ttcattcgcc atttaccttc ccgtctggtt catcgtgatc
     4741 cattgcctgg cgcgcagcag acagtgaatg cggcggtccc gccttcttta agtgcgcatt
     4801 gcctgaaaat ggcggtgatg cccgaagaag aattgtggaa aacgttcgac acccatccgg
     4861 aagggttaaa tctggcggaa gtggaatctg cccgcgaaca acatggtgaa aataaattac
     4921 ccgcacaaca accgtcgccg tggtgggtac atttatgggt ctgctatcgc aaccccttta
     4981 atattttgct taccattctc ggtgctattt cttacgccac ggaagattta tttgccgcag
     5041 gtgttatcgc gctaatggtc gctatttcta cgttgctgaa ctttattcag gaagctcgtt
     5101 ccaccaaagc ggcagatgcc ctgaaagcga tggtcagtaa taccgcgacg gtactgcgcg
     5161 taattaacga taaaggtgaa aatggctggc tggaaatccc gatagaccaa ctggtgcccg
     5221 gcgatattat aaaactggcg gcgggggata tgatcccggc agatttacgt atcttgcagg
     5281 cgcgggatct gtttgtcgct caggcgtcgt taaccggtga gtctctgccc gtagaaaaag
     5341 ccgctaccac tcgccagccg gagcacagca atccgctgga gtgcgacacc ctgtgtttta
     5401 tgggcaccac cgtggtgagc ggcacggcac aagcaatggt gattgccaca ggtgccaata
     5461 cctggtttgg tcaactggcg gggcgtgtta gtgagcagga aagcgagccg aatgcctttc
     5521 agcaagggat tagccgcgtc agtatgctgc tgattcgctt tatgctggtg atggctccgg
     5581 tggtgttgct tatcaacggt tacaccaaag gtgactggtg ggaagcggca ctgtttgcgc
     5641 tttcggtcgc ggtaggctta acgccagaaa tgttgccgat gattgttacc tcgacgctgg
     5701 cgcgtggcgc agtcaagttg tcgaaacaga aagtgatcgt caaacatctg gatgccattc
     5761 agaactttgg cgcaatggat attctgtgca ctgataaaac cggcaccctg acacaggata
     5821 aaattgtgct ggagaatcat accgatatct ccggtaaaac tagcgaacgc gtgctgcata
     5881 gcgcgtggtt gaacagccat taccagaccg ggcttaaaaa cctgcttgat acagcggtgc
     5941 tcgaaggtac ggatgaagag tcagcgcgct cgctggccag ccgttggcaa aaaattgatg
     6001 agattccgtt tgatttcgag cgtcgtcgga tgtcggtggt tgtggcagaa aataccgaac
     6061 accatcagtt ggtttgcaaa ggcgcattgc aggaaatcct caatgtgtgt tcgcaagtgc
     6121 gtcacaatgg cgagattgta ccgctcgatg acaccatgct gcgtaagatt aagcgggtta
     6181 ctgatacgct gaatcgtcag gggctgcgcg tggttgcggt ggcgacgaaa tatctgccag
     6241 cgcgtgaagg agattaccag cgggcggatg aatccgacct gatcctcgaa ggatatattg
     6301 ctttccttga tccgccaaaa gagacaacag ctccggcact gaaggcatta aaagcgagtg
     6361 gaattaccgt aaaaatcctt actggcgaca gtgagttggt tgcagcgaaa gtgtgccatg
     6421 aagtggggct ggacgcggga gaggtggtca ttggtagtga tattgaaacg ctatctgacg
     6481 acgaactggc aaatctcgca cagcgcacca cgctgtttgc tcgtctgacg ccgatgcata
     6541 aagaacgcat cgtaacgttg ctgaagcgcg aagggcatgt ggttggcttt atgggcgatg
     6601 gtattaatga tgcgcccgcc ttacgcgctg cggatatcgg catttctgtg gacggcgcgg
     6661 tagatattgc ccgtgaagcg gctgatatca tcctgctgga aaaaagcctg atggtgctgg
     6721 aagagggggt tattgaggga cgtcgcactt tcgccaacat gctgaaatac atcaaaatga
     6781 cggcgagctc taacttcggt aatgtgttca gcgtgctggt ggcgagtgct ttcttgccct
     6841 tcctgccgat gttgccgcta cacttgctta ttcaaaatct gctgtacgat gtgtcacagg
     6901 tggcgatccc atttgataac gtcgacgacg agcaaattca aaagccgcag cgttggaatc
     6961 cggcggatct ggggcgcttt atggtcttct tcggaccgat cagctcgatt ttcgatattc
     7021 tgacgttttg cctgatgtgg tgggtattcc atgccaacac gccggaaacg caaacgctgt
     7081 tccagtcggg ctggtttgtg gtgggcttac tgtcgcaaac gctgattgtg catatgatcc
     7141 gcacccgtcg tgtaccgttt attcagagct gtgcatcgtg gccgttaatg atcatgaccg
     7201 tgatcgtgat gattgtcggg atcgcattgc cgttttcacc gctggccagt tatctgcagc
     7261 tgcaggcgct gccgttaagc tatttcccgt ggctggttgc gattctggca gggtatatga
     7321 cgttaaccca gttggtgaaa gggttctata gccgtcgtta cggctggcaa taaagaatca
     7381 actgggcatg gtgacccgga ttcagattgc agacaaagtg agttttgttc atgccggatg
     7441 cggcgtaaat gccttatccg gccaataaat tcaataaatt gcaggaatca cgtaggcctg
     7501 ataagcgtag cgcatcaggc gattttgcgt ttgtcattag acttaatccg ggcatgatag
     7561 cccggatttc catcaagatt agcgacgaac agcgatcgct tcgatctcaa tcttcacgtc
     7621 tttcggcaga cgagcaactt caacgcaaga acgtgccggg aaggtggcgt tgtgttcggt
     7681 gaagaaggct tcgtaggtag cattaacggt tgcgaagtcg ttcagatctt tcacaaacac
     7741 ggtggtttta acgatatcgc ccactttcag gccagcggct tcgacgatcg cttttacgtt
     7801 atccagcgac tgacgtgcct gtgcagcgac atctgccggg acttcgcccg ttttcgggtt
     7861 taccgggatc tgaccggaag tgataatcat attgcccaga tcaacgccct gtacgtaagg
     7921 accgatagct gccggtgcat tttccgtcgc gatagttttg ctcatgattt ctcctttatt
     7981 acagcggtaa aaagtctggc tcattatagg gagccagact tttattacca accgcaatta
     8041 attggccagc accacattat gagaaaactc tttttcacag tatttgcatt tgagcgcgat
     8101 atcattggcg cgttttcgca cggcaaagct ggatgaaacc ggttcggcat ggctgataca
     8161 gttgctgtta gggcagacca gcacattgtc gatgcgctct ggcaaactgg ggcgcgattt
     8221 gccaaccact tcatagttgt cgatacggtt aaccgtggct tgcggtgcat ataacgccag
     8281 ctgatcaacc tgatcctcac ttaaaaaggt attttcgatt ttgatcaaat ctttgcggcc
     8341 catctcgcca gaaggcaaat tcagaccaat ggtgatgcgc tgatctgttt cggtcaactt
     8401 gaacagactc aacagcttaa aaccgatctg ggcggggata tggtcaatta ccgtgccgcg
     8461 tttaatagct tcaacctgca atttattatc gtgtgtcatc tctatttccc cttacagtac
     8521 cagatcgcga ttcagaacca gtgccagtaa cgcctggcga gcgaaaatcc cgttgcctgc
     8581 ttgctggaag taccaggcgt gaggcgtttt atccacatcc gtcgcaatct catcaacacg
     8641 cggcagcgga tgcagcactt tcatattggc tttggcgttg tggagatcgc tggcgcgaag
     8701 aacaaactgc gctttcacgt tggcgtactc ggacgggtcc agacgctctt tttgcacgcg
     8761 ggtcatgtac aggatgtcta cttccgccat cacttcttca atagagctgt gcagactcca
     8821 ggcgatccct ttttcatcga gcatatccag aatgtattgc ggcattgcca gcgcatccgg
     8881 cgcgataaag taaaaacggt tgccgtcgaa cttcgctagc gcctgggtca gggagtgaac
     8941 ggtgcggcca tatttcaggt caccaaccat tgcgacgtgg agattgtcca gacgcccctg
     9001 ggtttcctga atggtgaata agtccagcaa ggtttgcgtc ggatgttggt tggagccatc
     9061 accggcattc agtaccggta cattgccgga aaactcggtg gccaggcgcg ctgcaccttc
     9121 ctgcggatga cgcatcacta tcgcatcgac gtaagtgctg ataaccgaaa tggtatcggc
     9181 cagcgtttcg ccctttttac ccagtgatgt attggcgctg tcggagaagc ccaccacgct
     9241 ggcacccagg cggtgcatgg aagtttcgaa agagaggcgg gtgcgggtag aggcttcgaa
     9301 gaaacagctg gcaataactt tgtgcttcaa cagctctggt tgcgggttcg ctttcagttt
     9361 cgccgctgtc gccagcacca gattaaggtc atcgcgacta aggtcgttta tggaaatgat
     9421 atgtttctga tatagcggat tagccatctt ttatctcctg acgcctgggc aaaaaaaagc
     9481 ccctcgattg aggggctggg aatgggtgat caacgggaag aaaaacggca ggccagcgtc
     9541 ttttttcaga cgcggtaaga caaaatgtcg aacacactga accatacatc ctcccggcaa
     9601 attgtccggc attatactca gctctgagag aggatcaaga agatgatgca gttataaagc
     9661 aaggcaaacg gttatcatga atatatattc aattttaata agtaattaat ttgctt
//
LOCUS       DADSZD010000163         9521 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.164, whole genome shotgun sequence.
ACCESSION   DADSZD010000163 DADSZD010000000
VERSION     DADSZD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.164"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            119..682
                     /gene="srlA"
                     /locus_tag="J1Z28_003654"
                     /gene_synonym="gutA"
     CDS             119..682
                     /gene="srlA"
                     /locus_tag="J1Z28_003654"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBA7718452.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            679..1638
                     /gene="srlE"
                     /locus_tag="J1Z28_003655"
                     /gene_synonym="gutE"
     CDS             679..1638
                     /gene="srlE"
                     /locus_tag="J1Z28_003655"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBA7718453.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGEATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            1649..2020
                     /gene="srlB"
                     /locus_tag="J1Z28_003656"
                     /gene_synonym="gutB"
     CDS             1649..2020
                     /gene="srlB"
                     /locus_tag="J1Z28_003656"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBA7718454.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            2024..2803
                     /gene="srlD"
                     /locus_tag="J1Z28_003657"
     CDS             2024..2803
                     /gene="srlD"
                     /locus_tag="J1Z28_003657"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBA7718455.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            2909..3268
                     /gene="gutM"
                     /locus_tag="J1Z28_003658"
     CDS             2909..3268
                     /gene="gutM"
                     /locus_tag="J1Z28_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBA7718456.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMYAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            3335..4108
                     /gene="srlR"
                     /locus_tag="J1Z28_003659"
                     /gene_synonym="gutR"
     CDS             3335..4108
                     /gene="srlR"
                     /locus_tag="J1Z28_003659"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBA7718457.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            4101..5066
                     /gene="gutQ"
                     /locus_tag="J1Z28_003660"
     CDS             4101..5066
                     /gene="gutQ"
                     /locus_tag="J1Z28_003660"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBA7718458.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(5063..6577)
                     /gene="norR"
                     /locus_tag="J1Z28_003661"
     CDS             complement(5063..6577)
                     /gene="norR"
                     /locus_tag="J1Z28_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBA7718459.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGEAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            6764..8203
                     /gene="norV"
                     /locus_tag="J1Z28_003662"
     CDS             6764..8203
                     /gene="norV"
                     /locus_tag="J1Z28_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBA7718460.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLTDIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            8200..9333
                     /gene="norW"
                     /locus_tag="J1Z28_003663"
     CDS             8200..9333
                     /gene="norW"
                     /locus_tag="J1Z28_003663"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBA7718461.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDARIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
BASE COUNT         2274 a         2534 c         2523 g         2190 t
ORIGIN      
        1 ttcaaaataa aatatttatc tttcattttg cgatcaaaat aacactttta aatctttcga
       61 tctgattaga ttaggttgcc gtttggtaat aaaacaataa atactgaagg agagaacaat
      121 gatagaaacc attactcatg gcgcagagtg gtttatcggg ctgttccaga aaggcggaga
      181 ggtgtttacc gggatggtga ccggcattct tccgctgtta attagcctgc tggttatcat
      241 gaacgcattg attaatttta tcggtcagca tcgtattgaa cgatttgctc aacgttgcgc
      301 cggtaaccct gtttcccgtt atctgctgct accgtgcatt ggtacgtttg tcttttgtaa
      361 cccgatgacc ttaagtcttg gtcgctttat gccggaaaag tacaaaccca gctattacgc
      421 ggcggcctct tatagctgcc actcaatgaa tggcctcttc ccccatatca accctggcga
      481 actgtttgtt tatcttggca ttgccagcgg tctgacgacg ctgaatctgc cacttggccc
      541 gctggcagtg agttatctgc tggttggttt ggtcaccaac ttcttccgcg gctgggtgac
      601 cgatctgacc accgccattt ttgagaaaaa gatgggcatt caacttgaac aaaaagttca
      661 cctggcagga gcaacatcat gacacgcatt cggatcgaaa aaggaacggg tggctggggc
      721 ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg tctatatcac cgccggtacc
      781 cggcctgcga ttgttgacaa actggcacag cttactggct ggcaagctat cgacggattt
      841 aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca
      901 ttacgctgcg gcatctatcc gaaacggcgt attcccacca ttaatatcca ctcgacgggc
      961 aagtccggcc cgctggcgca gtacattgtg gaagatattt atgtctctgg cgtaaaagaa
     1021 gaaaacatca ctgtagtggg tgaggcgaca ccacaaccct cttccgtggg ccgtgactat
     1081 gacaccagca agaaaatcac cgaacaaagt gatggtttac tggcgaaggt gggaatgggt
     1141 atgggttctg ccgttgccgt gctgttccag tctggccgtg acaccatcga cactgtatta
     1201 aaaaccattc tgccgtttat ggcgttcgtc tcggcgctta tcggcatcat tatggcttct
     1261 ggccttggtg actggattgc ccacggtctt gctccgctgg cgagccatcc actgggtctg
     1321 gtcatgctgg cgctcatctg ctccttcccg ctgctttcac ctttcctcgg cccaggcgca
     1381 gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg
     1441 ccgcatctgg ctttacctgc actgtttgcc atcaacgcgc aggcggcctg cgacttcatc
     1501 ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg ttcgcgtcgg tgtcccttct
     1561 gttctggtga gccgcttttt aaccggcgcg ccaactgtac tgatcgcctg gtttgtctcc
     1621 ggttttatct atcaatagag gctgaaacat gaccgttatt tatcagacca ccatcacccg
     1681 tattggcgcg agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg
     1741 cgcgcctgcg gatctcgaag agtattgctt cattcattgc cacggcgagt tgaaaggtgc
     1801 actccatccc ggtttgcaat tttctctcgg gcggcatcgc tatccggtga ccgccgttgg
     1861 cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt
     1921 aagcgaagcg gaatttccgg gcactgtcca tgtggcaggc cctgtacccg acgatatcgc
     1981 gccgggatcg gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc aggttgccgt
     2041 tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc cacggtctgg ctgccgaggg
     2101 gtatcgcgtg gcggttgtcg atattcagag cgacaaagct gcaaatgtgg cgcaagaaat
     2161 taacgccgaa tatggtgaag gaacagcgta cggttttggt gctgacgcca ccagcgagca
     2221 gagcgttctg gcgctctctc gtggggtaga tgaaatcttt ggtcgcgtgg atttactggt
     2281 ctacagcgcc ggaatagcaa aagcagcctt tatcagcgat ttccagcttg gagattttga
     2341 ccgctcgcta caggtgaatc tggtgggcta tttcctgtgt gcgcgtgaat tttcgcgttt
     2401 gatgatccgc gacggcattc agggacgcat tattcagatc aactcgaaat ccggcaaagt
     2461 gggcagcaaa cacaactctg gctacagcgc agcgaaattt ggtggcgtcg gactgactca
     2521 atcactggct ctggatctgg cggagtacgg cattacggtg cattcactaa tgctcggtaa
     2581 tctgctgaaa tcgccgatgt tccagtcact gttgccacaa tacgcgacca agctgggtat
     2641 caaaccggat caagtcgagc agtattacat cgataaagta ccgctcaaac gcggctgcga
     2701 ttatcaggat gtgctgaata tgctgctgtt ctacgccagt ccaaaggcat cgtactgcac
     2761 tggacagtcg attaatgtca ccggcggtca ggtgatgttc tgatcaacag cggagatccg
     2821 ttaaggatct ccgcgagact aatagaatgc ctgatgcgct acgcgcatca ggcatacagg
     2881 actttcgcca ctacattaag gaaaagttat ggtatccgca ctcatcaccg tcgccgttat
     2941 cgcctggtgt gcgcaactgg ccttaggcgg ctggcaaatt tctcgtttta accgtgcctt
     3001 cgacacacta tgccagcaag ggcgggttgg cgtgggccgt tccagcgggc gctttaaacc
     3061 gcgggtcgtg gtcgccatcg cgctggacga tcagcaacgc atcgtcgaca ccttgtttat
     3121 gaaaggactg accgtcttcg cccgaccgca aaaaattccc gcaattaccg gtatgtatgc
     3181 gggtgattta cagcccgatg tgatctttcc ccatgatcca ctatcacaga atgctctatc
     3241 attggcgctt aaactgaaac gtggataatt tcgttgtgaa tgttacttgc ttgcgaagtt
     3301 atcattttga aacctaaatc aggtaatcac gcccatgaaa cctcgtcagc gtcaggccgc
     3361 cattctggag tatctgcaaa agcagggtaa atgctcggtt gaagaattgg cgcaatactt
     3421 tgacaccaca ggcacaacca ttcgcaaaga tctggtcatt ctggaacatg caggaaccgt
     3481 cattcgtact tatggcggag tggtgttgaa taaagaggaa tccgatccgc ctatcgatca
     3541 taaaacactc atcaacaccc acaagaaaga gctgattgca gaagctgccg ttagttttat
     3601 ccatgatggc gattcgatca ttcttgatgc tggcagtacc gttttgcaga tggtccccct
     3661 gctctcgcgc tttaataaca tcacagtaat gaccaacagc ctgcatatcg tcaatgcgct
     3721 atccgaactg gataacgaac aaactatcct gatgccaggc ggaacatttc gcaaaaaatc
     3781 ggcctcattt cacgggcaac tggcagagaa tgccttcgag catttcacct tcgataaatt
     3841 gtttatgggc accgacggca tcgatctcaa tgcgggcgta accaccttta acgaggtcta
     3901 taccgtcagt aaggcgatgt gtaatgccgc gcgcgaagtg attttgatgg ctgactcatc
     3961 gaagtttggt cgtaaaagcc ccaacgtggt ttgtagtctt gaaagcgtcg ataagctgat
     4021 taccgacgca ggtatcgatc cagcgtttcg tcaggcgctg gaagaaaaag ggatcgacgt
     4081 gatcataacc ggagagagca atgagtgaag cactactgaa cgcgggacgt cagacgttaa
     4141 tgctggagtt gcaggaagca agccgtttac cagaacgtct gggcgatgat tttgttcgcg
     4201 ccgccaatat catcctgcac tgcgaaggca aagtggtggt ttcgggaatt ggcaaatcgg
     4261 gccacattgg taagaaaatc gcggcaacgc ttgccagcac cggcacaccg gctttttttg
     4321 tccatccggc agaagcactg cacggcgatc tggggatgat tgaaagccgc gatgtgatgc
     4381 tgtttatctc ttattccggt ggcgcgaagg aactggatct gattattccg cgtctggaag
     4441 acaaatctat cgcgctgctg gcaatgaccg gaaaaccgac gtcaccgctg ggcctggcgg
     4501 caaaagcggt gctggatatc tccgtagaac gcgaagcctg cccgatgcac cttgcgccga
     4561 cttccagcac cgtgaatacc ctgatgatgg gcgacgcgct ggcgatggcg gtcatgcagg
     4621 cgcgcggatt taatgaagaa gattttgccc gctcccaccc agccggggca ctgggcgctc
     4681 gcttgctgaa caaagtgcat catcttatgc gtcgtgacga tgccatcccg caggtggcgt
     4741 taaccgccag cgtgatggac gcgatgctgg aacttagccg caccggttta ggtctggtgg
     4801 cagtgtgcga tgatcaacga ctggtgaaag gcgtctttac cgacggtgat ttacgtcgct
     4861 ggctggttgg cggcggcgca ctcaccacgc cagtcaatga agcgatgacg gtcggcggca
     4921 ccacgttgca atcgcaaagc cgtgccatcg acgccaaaga aatcctgatg aaacgcaaaa
     4981 tcaccgccgc accggtagtg gatgaaaacg gcaaactcac cggcgcaatt aacctgcagg
     5041 atttctatca ggccgggatt atttaatcct tcagccccag acgtttcgcc agccgatgca
     5101 ggttggcgac gtcggtttcc agcatccgcg cacaggcggc ccagttgtga tgattttgcg
     5161 ccagtgcctg acgaatggtt tcacgctgga acgcttctgt cgcttcacgc aggttttgct
     5221 taacaacggg caccgccgcc gcttctggcg gcggcaacgt cacctcagga aaagcaaaat
     5281 gttgcgcctc aagaatcact tcatcgccgc tgcgggtggc tctcgccagc actaccgccc
     5341 gatgaatagc atgttccagt tcacgaacgt tccccggaaa attgtagtgt tgcagtaaat
     5401 ttcgcgctcc ggcacttaat accactcggg agagccccag ccgcaaacga cactgctcgc
     5461 agaaataccc cgccagcaga atgacatcat cgccccgctc acgcagcggc ggcactgaaa
     5521 gtggaaacac gctcaggcgg tggaacaagt cagcgcgaaa tcgccctgcc agtacctctt
     5581 cgcgtaagtc acggttagtc gccgccagca cgcgcacatc gacccgcaaa ctgcggtcat
     5641 cgccaacgcg ttgaatatcg ccatactgca acacccttag cagcttggcc tgcaatgcca
     5701 acgacaactc gccgatctca tcgagaaaca gcgtaccgtt atccgccatt tcaaacttcc
     5761 cgctgcggtt actgatagca ccagtaaacg cccctttcac atgcccgaac aactcacttt
     5821 ccgccacact ttccggcagt gcggcacagt tgagatagac cagcggattc accgcccgtg
     5881 gcgaggcttc atgaatcgct ttcgccacca gctccttacc ggttcccgtc tcaccgctaa
     5941 ttaaaacgtt gagatcggat gccgccacaa tctcaatctc ttttttcaat tgcgtcatgc
     6001 ctggcgacag accgatcatc tgcgtctgtt tcaccgcttc aaacggcgcg gcttcgcctg
     6061 gcagcatatt ctggctttcc agttgttcaa tcagtaacgc attgcttaac gctcccgccg
     6121 ccagcgcagc aatcaggcgt aactcttcgt cgctgaaaac atcgaactga tcgggctgca
     6181 tcccgtcgag cgtcaaggca ccgatcaggt tttgcccggc aaacaacggc aaaccaacgc
     6241 aggcgtgaac cttcagactc tcttgccctg gaatcagacc gtcataagga tcgggcaatt
     6301 cgctgtctgc gggaaagcgc accacatcac ccgcgcgggc aatcgcttcc agccgtggat
     6361 gaccttccag cgcaaagcgt ctaccgagta catccttcgc cagcccgtcg atggcaagcg
     6421 gaataaactg ccgcgagtcg taacgtagca acgcagacgc atcgcactcc agcacctgac
     6481 gcagcgtggt gatcaggcgc tgaaaacgat cctgatgacc aatcccacgc tgcaattcga
     6541 tggcgatatt cgccagcaca tcaacggaaa aactcatctt tgcctcactg tcaatttgac
     6601 tatagatatt gtcatatcga ccattttatt gatagtcatt ttgactactc attaatgagc
     6661 acaattttat ttatagagta aaaacaatta gataaaaaac tggcacgcaa tctgcaatca
     6721 gcaagacatc tttttagaac acgctgaata aattgaggtt gctatgtcta ttgtggtgaa
     6781 aaataacatt cattgggttg gtcaacgtga ctgggaagtg cgtgattttc acggtacaga
     6841 atataaaacg ctgcgcggca gcagctacaa cagctacctc atccgcgaag aaaaaaacgt
     6901 gctgatcgac accgtcgacc ataaattcag ccgcgaattt gtgcagaacc tgcgtaatga
     6961 aatcgatctg acggatatcg attacatcgt aattaaccat gctgaagagg accacgccgg
     7021 ggcgctgacc gaactgatgg cacaaattcc cgatacgccg atctactgta ctgccaacgc
     7081 tatcgactcg ataaatggtc atcaccatca tccggagtgg aattttaatg tggtgaaaac
     7141 aggcgacacg ctggatatcg gcaacggcaa acagctcatt tttgtcgaaa cgccaatgtt
     7201 gcactggccg gacagcatga tgacttacct gacaggcgac gcggtgctgt tcagtaacga
     7261 tgctttcggt cagcactact gcgacgaaca tctgttcaac gatgaagtgg atcagactga
     7321 gcttttcgag cagtgccagc gttactacgc caatatcctg acgccgttca gccgcctggt
     7381 aacgccgaaa attaccgaga tcctgggctt taacttgcca gtcgatatga tagcgacctc
     7441 tcacggcgtg gtatggcgcg ataacccgac gcaaattgtc gagctgtacc tgaaatgggc
     7501 ggcggattat caggaagaca gaatcaccat tttctacgac accatgtcga ataacacccg
     7561 catgatggct gacgctatcg cccaggggat tgcggaaacc gacccacgcg tggcggtgaa
     7621 aattttcaac gtcgcccgaa gcgataaaaa cgaaatcctg accaatgtct tccgctcaaa
     7681 aggcgtactg gtcggcactt ctacgatgaa taatgtgatg atgccgaaaa tcgccgggct
     7741 ggtggaggag atgaccggat tacgcttccg taacaaacgc gccagcgctt tcggctctca
     7801 cggctggagc ggcggtgcgg tggatcgtct ttccacacgc ctgcaggatg cgggtttcga
     7861 aatgtcgctt agcctgaaag ccaaatggcg accagaccag gacgctctgg agttatgtcg
     7921 tgaacacggt cgcgaaatcg cccgtcagtg ggcgctcgcg ccgctgccgc agagcacggt
     7981 gaatacggtg gttaaagaag aaacctctgc cgccacgacg gctgacctcg gcccacggat
     8041 gcaatgcagc gtctgccagt ggatttacga tccggcaaaa ggcgagccaa tgcaggacgt
     8101 tgcgccagga acgccgtgga gtgaagtccc ggataacttc ctgtgcccgg aatgctctct
     8161 cggcaaagac gtctttgacg aactggcatc ggaggcaaaa tgagcaacgg cattgtgatc
     8221 attggttcgg gcttcgccgc ccgccaactg gtgaaaaata ttcgcaaaca ggacgccaga
     8281 attccattaa ccctgattgc cgccgacagc atggatgagt acaacaaacc tgacctcagc
     8341 catgttatca gtcaggggca acgtgccgac gaccttaccc gccagacggc aggtgaattt
     8401 gccgagcagt ttaatctgcg cctgtttccg cacacctggg taacggatat cgatgccgaa
     8461 gcccatgtgg tgaaaagtca gaataatcag tggcaatacg acaagttagt gctggcaacc
     8521 ggtgccagcg cctttgtccc gccagtgccc gggcgtgagt taatgctgac gttaaatagt
     8581 cagcaagagt atcgcgcctg tgaaacgcaa ctgcgggatg cccgacgcgt gttgattgtc
     8641 ggcggtggtt tgattggtag tgaactggcg atggattttt gtcgggcagg caaagcggtc
     8701 acgctaatcg acaacgctgc cagtattctg gcgtcgttaa tgccaccgga agtcagcagc
     8761 cgcttgcagc atcggttgac ggagatgggc gttcatctgc tgttgaaatc tcagttgcag
     8821 ggactagaaa aaacagattc tggcattctg gcaacgctgg accgccagcg ctgcatcgaa
     8881 gtggatgcgg taattgccgc caccggactg cgcccggaaa ccgccctggc acgacgcgcc
     8941 gggctgacga ttaatcgcgg cgtttgcgtc gatagttatc tgcaaaccag taatgccgat
     9001 atttatgcgc tgggcgattg cgcggaaatt aacggtcagg tattgccgtt cctccagccg
     9061 attcaactta gcgcgatggt gctggcaaaa aatcttctcg gcaataacac gccgctgaaa
     9121 ctcccggcga tgctggtgaa aatcaaaacg ccggaattac cgctgcatct ggcaggcgaa
     9181 acccagcgtc aggatttacg ctggcaaatt aataccgaac gccagggaat ggtggcacgc
     9241 ggcgttgacg atgctgacca gcttcgcgcc tttgtggtca gtgaggatcg gatgaaagag
     9301 gcatttggat tgttgaaaac gttgtcgatg taggcgagct acagtgcccc aaatgtcgga
     9361 tgcggcgctg acgcgtctta tacgacctac agggaacgca tgtgttgatc acataatacg
     9421 tttatgtcgc attgcactgt cccaaaatgc cggatgcgac gctggcgcgt cttatccgac
     9481 ctacggggac gcacgtgtag gccggataag gcgtttacgc c
//
LOCUS       DADSZD010000164         9276 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.165, whole genome shotgun sequence.
ACCESSION   DADSZD010000164 DADSZD010000000
VERSION     DADSZD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.165"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(41..1261)
                     /locus_tag="J1Z28_003664"
     CDS             complement(41..1261)
                     /locus_tag="J1Z28_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718462.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            1479..3131
                     /locus_tag="J1Z28_003665"
     CDS             1479..3131
                     /locus_tag="J1Z28_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBA7718463.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYGPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3168..3647)
                     /gene="ybaK"
                     /locus_tag="J1Z28_003666"
     CDS             complement(3168..3647)
                     /gene="ybaK"
                     /locus_tag="J1Z28_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBA7718464.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(3851..4645)
                     /locus_tag="J1Z28_003667"
     CDS             complement(3851..4645)
                     /locus_tag="J1Z28_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718465.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEDQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPSGRYVVAVGALHLYGEGNLPQMLR"
     gene            4778..5080
                     /locus_tag="J1Z28_003668"
     CDS             4778..5080
                     /locus_tag="J1Z28_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7718466.1"
                     /translation="MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIIN
                     AATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYRLIFKWVNGKAEELFLDPHNY"
     gene            5116..5457
                     /locus_tag="J1Z28_003669"
     CDS             5116..5457
                     /locus_tag="J1Z28_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBA7718467.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5515..8019)
                     /gene="copA"
                     /locus_tag="J1Z28_003670"
     CDS             complement(5515..8019)
                     /gene="copA"
                     /locus_tag="J1Z28_003670"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBA7718468.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALVVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     NVPLADVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVDTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            8281..9213
                     /gene="glsA"
                     /locus_tag="J1Z28_003671"
     CDS             8281..9213
                     /gene="glsA"
                     /locus_tag="J1Z28_003671"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBA7718469.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVS
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT         2349 a         2423 c         2406 g         2098 t
ORIGIN      
        1 aaaacgatgc agccaaagtt tgcctttggc tgcaatgaaa tcagtcttta tgccggttat
       61 caggcaggaa tatggtcaac atccccaata gtggcaggaa agcacagatt ttatagacta
      121 actctatgct ggtgtggtcg gcgataagcc ccagaactgc cgcccccaga cctcccatgc
      181 cgaaagcaaa accgaaaaat agtccagaaa ccataccgat acgtcccgga agcagctcct
      241 gggcatagac cagaatggca gagaatgccg aagcgaggat aaatccaata atcaccgtta
      301 aaacccccgt ccagtgcagg ctggcgtagg gtaaaatcag cgtaaacggc gcaacgccga
      361 ggatagagcc ccaaatcaca tatttccgtc caattttatc ccctacaggc ccgccaatca
      421 ccgtacctgc cgcaacggca aacaggaagg caaacaaatg aagctgagca ttctggatag
      481 ataatccgaa tttttgcatc agataaaagg tgtaatagct gctgatgctc gccatataga
      541 aatatttcga gaaaatgagg attaacagaa tgctgaccgc cagaacaact ttattgcgcg
      601 gcagtggatt gataatcgtc gctttgggtt ttcctttatt cattcggtgc tgtgccgagt
      661 accaacggct gatttgcgcc aacaccacga tcgccagcag tgccgcaagc acaaaccagg
      721 caacgttgcc tttgccataa ggcgcgataa tcaccgccgc cagcaagggg cccagggaac
      781 tgccaaagtt gccgccgacc tgaaagatag attgcgccag gccatgacgc ccgccggaag
      841 ccatacgggc cacgcgagaa gattccggat gaaagaccga tgaaccggta ccgaccagcg
      901 ccgctgccag cagaactgcg ccgaaactgc ccgccagcgc cagcagcacc agaccactta
      961 aggtaaagca catgccaatc ggcaacgacc acggcatcgg atatttatcg gtccagtagc
     1021 cgaccaccgg ttgcagtaac gaagaggcga gctggaaggt gagggttatc atgccaatct
     1081 gcataaatgt cagagaaaat tctgactgaa gcagtggata aatcgccaga atcagcgatt
     1141 ggatcatgtc gttcagcaga tgtgagaggc tgatagcacc taaaatacca aacgatgttc
     1201 gggccttagt cgttgacgca gccgcgcccg ccacaggctg ggtttgttca ctcattgcca
     1261 taggaaagtc actttttcag ggttgcgatg taaagaatga tcttatttgt gattattacc
     1321 cgactaacat acctgtatgc gccgcctgaa ggaagtctca acgcggaata cagaatttct
     1381 aatctggatg cagatttatc ttcaccggac gcagacttgt ttatgatgtc gcgtcatact
     1441 atttttcaac acgttgaaat caggtcaggg agagaagtat gaaattattg cagcggggcg
     1501 tggcgttagc actgttaacc acatttacac tggcgagtga aactgctctg gcgtacgagc
     1561 aggataaaac ctacaaaatt acagttctgc ataccaatga tcatcatggg catttttggc
     1621 gcaatgaata tggtgaatat ggtctggcgg cgcaaaaaac gctggtggat ggtatccgca
     1681 aagaggttgc ggctgaaggc ggtagcgtgc tgctactttc cggtggcgac attaacactg
     1741 gcgtgcccga gtccgactta caggatgccg aacctgattt tcgcggtatg aatctggtgg
     1801 gctatgacgc gatggcgatc ggtaatcatg aatttgataa cccgctcacc gtattacgcc
     1861 agcaggaaaa gtgggctaag ttcccgttgc tttccgccaa tatctaccag aaaagtaccg
     1921 gcgagcgcct gtttaaacca tgggcgctgt ttaagcgtca ggatctgaaa attgccgtta
     1981 ttggcctgac gacggatgac acagccaaaa ttggtaatcc ggaatatttc actgatatcg
     2041 aatttcgtaa gcccgctgat gaagcgaagc tggtgattca ggagctgcaa cagacagaaa
     2101 agccagacat tattatcgcg gcgacccata tggggcatta cgacaatggt gagcacggct
     2161 ctaacgcacc gggcgatgtg gagatggcac gcgcgctgcc tgccggatcg ctggcgatga
     2221 tcgtcggtgg tcactcgcaa gatccagtct gtatggcggc agaaaacaaa aaacaggtcg
     2281 attacgggcc gggtacgcca tgcaaaccgg atcaacaaaa cggcatctgg attgtgcagg
     2341 cgcatgagtg gggcaaatac gtgggacggg ctgattttga gtttcgtaat ggcgaaatga
     2401 aaatggttaa ctaccagctg attccggtga acctgaagaa gaaagtgacc tgggaagacg
     2461 ggaaaagcga gcgcgtgctg tacacccctg aaatcgctga aaaccagcaa atgatctcgc
     2521 tgttatcacc gttccagaac aaaggcaaag cgcagctgga agtgaaaata ggcgaaacca
     2581 atggtcgtct ggaaggcgat cgtgacaaag tacgttttgt acagaccaat atggggcggt
     2641 tgattctggc agcacaaatg gatcgcactg gtgccgactt tgcggtgatg agcggaggcg
     2701 gaattcgtga ttctatcgaa gcaggtgata tcagctataa aaacgtactg aaagtgcagc
     2761 cattcggcaa tgtggtggtg tatgccgaca tgaccggtaa agaggtgatt gattacttga
     2821 ccgccgtcgc gcagatgaag ccagattcag gtgcctaccc gcaatttgcc aacgttagct
     2881 ttgtggcgaa agacggtaaa ctgaacgacc tgaaaatcaa aggcgaaccg gtcgatccgg
     2941 cgaaaactta ccgcatggcg acattaaact tcaatgccac cggcggggat ggctatccgc
     3001 gccttgataa caaaccgggc tatgtgaata ccggctttat tgatgccgaa gtgctgaaag
     3061 cgtatatcca gaaaagctcg ccgctggatg tgagtgttta tgaaccgaaa ggtgaggtga
     3121 gctggcagta atccgaaagt gccggatgtt cgcatccggc acaatgctta atcgcgacgg
     3181 gcgatatcag caaatttggc atcgagtatc tttgccaggt cgcttgccgc cagttcgata
     3241 tccagaccgc gcttgccgcc ggaaacataa atagtggcaa attcttgtgc gggggcgtcg
     3301 ataatcgttg gcagacgttt tttctgcccc agtgggctaa tccctccaac cagatatccc
     3361 gtcgaacgct gcgcgaccat cggatcggcc atctcaactt tcttggcacc cagcgctttt
     3421 gctacttttt taagatccag ttgacctgcg accggcgtaa cggccacggc aaggtgtttc
     3481 atatcaccgt tcactgcgac cagcagcgtt ttgtagacct gatccggatt caaacctaat
     3541 tttttgacga cttcatcgcc aaaattggtt tcagccggat cgtgctcgta ggtgtggatt
     3601 tgaaacgaaa tcttgttttt ttcgagtaat ttaactgcgg gtgtcatggt aattttcctg
     3661 ccttaaacaa aaaaacgaag caagcatacg cctgatggcg gtccaaaaaa aagagtcatc
     3721 ttgcctaaga gtattggcag gatggtgaga ttgagcgaca atcgagttac accgtcgctt
     3781 aaagtgacgg cataataata aaaaaatgaa attcctcttt gacgggccaa tagcgatatt
     3841 ggccattttt ttagcgcaac atctgcggca aattcccttc gccatacagg tgtagtgcac
     3901 caaccgcgac cacatagcgc cccgacggca tggcgcgtaa tttatcccgc caggcgagat
     3961 ttcgctgatg catcagcaca tcgtacagcg actgactgaa cgtattgggc aacgttattt
     4021 cattattttg cggcggtgca ttcagccacc agctcatcat ttgttgcagc aaccgtgcgt
     4081 tggtgtgcca gtgggtcagc gtatcgtcca gcagcgccag tcctttgtca gggagctgga
     4141 gcaacatggc aatctggttt tcagcccctt ccagttcaat cacgggttta tgttgttgct
     4201 tagccgcctg caatagctgg taatcgatac cgtattctgc ccgcagcccc agtttttgcg
     4261 cctgcgtcgc ctgaagaacc atcgcgattt gccacagcgg ttgggtagaa aagagcgacg
     4321 gagaaatgcc catctcctgg ctaatgtgct gcaggttttg gagttggtcc tcgctaatgc
     4381 gctcttccag ggcctcgcag gcaggcaaat tggcaaaagg cgtatcgctg gtggaaacat
     4441 ccgcctcgac gatcagcgca tcggcgtttt tgagcttttt gagcaaacgg gtgggcaggg
     4501 gagccatatc gtggctaccc atatgaatac tgccgatcag atgaaaatgg cgattgccgg
     4561 gaagagagat atcgatagca ggccaggagt aatgattacc gcgcagcgcg gcccaaagcg
     4621 tttttacccg gtacaacaga tccatacgac ctccctttgt gaaatatcat gctagcgcgc
     4681 ggggagggat ggcgcaaatg gagttgattg ttgcacttgc gcacagtgct tgtgtgcatt
     4741 acaatgatta aataatggac aaggagatga gagaggaatg gctcaaaaga aaaatataag
     4801 gagttttcgt gatgcctggt tggctgattt ttttgtccat tcaacgccac atcgaaagat
     4861 ccctgctgaa atccatacca cgttatcacg aaagttggac atcattaatg ctgccacttc
     4921 tcatcgggat ttacgctccc ctccggggaa ccgatacgaa gagttatccg ggaaattaca
     4981 ggaatactcc tccattagag tgaataaaca gtaccgactg atttttaaat gggttaatgg
     5041 caaggccgaa gaattgtttt tagacccaca caactactaa aacatagctg tcgtattgac
     5101 gagaaaggta aaactatgaa acaggcaaca agaaaaccga cgacacccgg agatattctt
     5161 ctctatgaat atctggaacc gctcgatttg aaaatcaatg agttagcaga gttgctgcat
     5221 gttcatcgta atagcgtcag tgcactgatc aataacaatc gtaaactcac cactgagatg
     5281 gcattccgtc tggcgaaagt ttttgatacc acagtcgatt tttggctaaa cctccaggcg
     5341 gcagttgatc tttgggaagt tgaaaacaac atgcgtaccc aggaagaatt gggacggatt
     5401 gaaacagtgg ctgaatattt ggcacgccgt gaagagcgtg caaaaaaggt cgcgtagaat
     5461 aatttcccta taacggatgc gccaccctaa agcagcgcat ccgcaatgat gtacttattc
     5521 cttcggttta aaccgcagca agcggttggc gttactcact acggtgatcg acgagagcgc
     5581 cattgccgct ccggcaacca ccgggttaag cagtgttcca gtgaacggcc acaaaatacc
     5641 ggcggcgacc ggaataccaa tgctgttgta gataaacgca ccgagcaggt tctgcttcat
     5701 gttgcgcagc gttgcgcggg aaattgcgag cgcatccgca acacccatca ggctatggcg
     5761 catcagggta atcgctgcgg tttcaatggc aacatcactg ccgcccccca tcgcaatacc
     5821 gacatccgcc tgagccagcg ctggcgcatc gttaatgccg tcgcccacca ttgctacctg
     5881 acgtccttca ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcacgccggc
     5941 tatcacctca tcaatccctg cttctttagc gatcgcattg gcggtggtcg ggttatcccc
     6001 ggttagcatc accaggcgat atcccgcttt atgcaggcgt tgcagcgccg ccacgctatc
     6061 actacgcaat ggatcgcgta cagccagcag ggctaccgct ttcccgtcaa ccgccagcag
     6121 cacaggcgtt gccccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtatc
     6181 aacctgttgc tcatttaaca acgcctgatt acccagcaat aacgcatgac cttcagcttc
     6241 accgctcacg cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc
     6301 acctgcttta tcgaggatcg ctcgtgccag cggatggctg gaaccttgct ccagcgccgc
     6361 cgccagacgc aatgcttgcg cttcatcaac atcagcaaac gtttttactg cgacaacctg
     6421 cggtttacct tcggtcagcg tcccggtttt atcgaacact acggtgtcga gcgtactggc
     6481 gcgttgcagc gcgtcggcgt cccgtaccag cacgccaaac tcagccgccc gcccgacgcc
     6541 cgaaataatc gacatcggcg tcgccagccc cagcgcacac ggacaggcaa taatcagcac
     6601 cgtggtggca atcaccaggg tatagacaat ctgcggtgcc ggaccaaaga aataccagat
     6661 tgccgcactg atcagcgcaa tcaccaccac taccggcaca aatacggctg agattttatc
     6721 cgccagctga ccgatttctg gcttactgct ctgggcctgg cgcaccatac gaataatccg
     6781 cgacagcgta gtatggctgc caaccgcact ggcgcgaaac agcacgctac cgtcctgcac
     6841 cactgtcccg gcatggacgc tatcgccttc gcctttttgc tgcgggatag gttcgcccgt
     6901 cagcatcgct tcatccagcc acgcttcgcc ttgagtaatc tcaccatcta ccggcacacg
     6961 gtcgccggtc gtcaggcgca gtaacatacc tggctgaaca tctgccagcg gcacgttttt
     7021 ttcaccttcg tcggtgacca ggcgcgccgt cggtggggtt aaatcgagta atttctccag
     7081 cgccttagaa gaacgctgac gtgcgcgcgc ttccagcata tgaccgagat taatcagacc
     7141 gataatcatc gcactggctt catagtaaag atgccgtgct tccatcggga accactgcgg
     7201 ccacaggttg acgctcatcg aatagagcca cgcaacaccc gttcccagcg ccaccagcgt
     7261 atccatcgtc gccgcaccgt tcagcaggct tttccatgcg ctgcggtaaa aatgcccgcc
     7321 ggcgaaaacc attaccgcca gggttatcag gccgataact aaccacaggc tgcggttgtc
     7381 agcggtgacc atcatgttat cgccgatcat cccccagacc atcaccggga tacccaccac
     7441 cagcgcgaca attgcctgcc agcggaagcg cttcatcgta gcgacggcgg tttcttgctg
     7501 gcgctcgcgg cgtttagcgt catcttcaat cgcttccgcg ccgtagcccg ctttttccac
     7561 cgcctgcact aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag
     7621 gtttacccgt gcctgagtga cgcccggtac gctttgcaac gcattttgta cgcgggtgac
     7681 acagctggcg cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt
     7741 cgctgccgga agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc
     7801 cagcggttta gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc
     7861 gatcagctgt tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc
     7921 ctgttcaaca tccgggcgct gttcgagact ttctttcacg cgtttaacgc agtgaccgca
     7981 ggacaggcca tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt
     8041 tttgactggc tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa
     8101 accaggcggg taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga
     8161 catttatctc tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcat
     8221 gaaaatcggc accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag
     8281 atgttagatg caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca
     8341 cttaacggcg gacaaaatgc cgattacatt ccctttctgg cgaatgtccc tggtcaactg
     8401 gcggcagtgt ctatcgtgac cagcgatggc aacgtctata gtgcgggtga cagtgattac
     8461 cgctttgcgc tggaatccat ctcgaaagtc tgtacgttag cccttgcgtt agaagatgtc
     8521 ggcccgcagg cggtacagga caaagttggc gctgacccga ccggattgcc ctttaactca
     8581 gttatcgcct tagagttgca tggcggcaaa ccgctttcgc cactggtaaa tgctggcgct
     8641 attgccacca ccagcctgat taacgctgaa aatactgaac aacgctggca gcgaatttta
     8701 catatccaac aacaactggc tggtgagcag gtagcgctct ctgatgaagt caaccagtcg
     8761 gaacaaacaa ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat
     8821 ctctattgtg atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc cacgctcatc
     8881 aatactattg aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgctg
     8941 acgcataaac gcgttcttca ggccgacaat gtgccgtaca ttctggccga aatgatgatg
     9001 gaagggctgt atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc
     9061 ggtgtaggtg gcggtattct ggcggtcgtt cctggcgtga tgggaattgc cgcgttttca
     9121 ccaccgctgg acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct
     9181 aagcaactcg gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg
     9241 gtaacaaacc cctcggtcta tggaacgtcg tttcca
//
LOCUS       DADSZD010000165         9206 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.166, whole genome shotgun sequence.
ACCESSION   DADSZD010000165 DADSZD010000000
VERSION     DADSZD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.166"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..280
                     /locus_tag="J1Z28_003672"
     CDS             <1..280
                     /locus_tag="J1Z28_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718470.1"
                     /translation="VGNAVILLAKDVIYNEYFFDLRKSFFVSVKDLMASGTKGIPETQ
                     KYPDVYCWIPLFSINYGVVITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            664..870
                     /locus_tag="J1Z28_003673"
     CDS             664..870
                     /locus_tag="J1Z28_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA7718471.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            964..1566
                     /locus_tag="J1Z28_003674"
     CDS             964..1566
                     /locus_tag="J1Z28_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001596160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718472.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIHYFHNLT
                     PGWVSFNGEKPEIAIVPRSLHRLIYGSDKRATPPLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTENLKRK
                     SEKELGEWIIDRQKQNAALADLMDQYVLLAYRTTVDDNRN"
     gene            3276..3488
                     /locus_tag="J1Z28_003675"
     CDS             3276..3488
                     /locus_tag="J1Z28_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718473.1"
                     /translation="MDELKSAREQQKRRDERADGSVLLKLSGKIHKQLESIARSRGVP
                     PKKMLNEMIEQAHRDFVANEQHKTRS"
     gene            complement(3981..4238)
                     /locus_tag="J1Z28_003676"
     CDS             complement(3981..4238)
                     /locus_tag="J1Z28_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718474.1"
                     /translation="MQHIDRLNVIKALVLLEDEQIVRFNIAANDNASQIHMLVDGLGV
                     GLTHEPVAAGMISHRTACHRSRQHDRPAHSYFYYLSAYQVA"
     gene            complement(4292..5059)
                     /locus_tag="J1Z28_003677"
     CDS             complement(4292..5059)
                     /locus_tag="J1Z28_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBA7718475.1"
                     /translation="MSGLTINALCAGYGKRRIIEHLSISTLPRGEVTVLLGPNGCGKS
                     TLLRALAGLNRASGEAWLNEENLLSLPFARRAEKVVFLPQSLPQGVHLQVLESVVVAQ
                     RASGAGQNQAQAIALLEELGIAHLAMNYLDSLSGGQKQLVGLAQSLIRRPALLLLDEP
                     LSALDLNYQFHVMDVVSRETRRRNMVTLVVLHDINIALRHAAQVIMLKEGKLIDSGDP
                     QTVIHAESLAQVYGVRGRVERCAQGRSMVIVDGAIEK"
     gene            complement(5056..6114)
                     /locus_tag="J1Z28_003678"
     CDS             complement(5056..6114)
                     /locus_tag="J1Z28_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBA7718476.1"
                     /translation="MTVLESTLSVENRYRQLFRQRLVILAVIFIAIIASLLLDFTLGP
                     SGLPLHSLIKTLMHPSGATNGMRVIVWDIRLPYALMALLVGMALGLAGAEMQTILNNS
                     LASPFTLGVSSAAAFGAALAIVLGIGIPGVPESGFIPANAFLFALLSALLLDSLTRWT
                     RVPTSGVVLFGIALVFTFNALVSIMQFVADEDTLQGLVFWTMGSLARASWEKLAVLAA
                     AMAIVLPWSLRRAWQLTALRLGEERAMSFGIDVRRLRLGSLLRISLLAALSVAFVGPI
                     GFIGLVAPHISRLLLGEDHRFYLPGSILIGGLVLSLASVASKNIIPGVILPVGIVTSL
                     VGVPFFLSIVMRHRGSMS"
     gene            complement(6133..7122)
                     /locus_tag="J1Z28_003679"
     CDS             complement(6133..7122)
                     /locus_tag="J1Z28_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBA7718477.1"
                     /translation="MKLSRLFPLLALLLAASLHAENTHKEVRIASPWPAQNTIIAMLG
                     YGDNIVGTSMIAKRIPLFRQSLPRIEKVAAVSVNSGHEINPEQIIALGVDMLFVPQNM
                     VVPQQALLKQAGVQVLAFEANSLRALTQRVQQTAAVLGPDAQQKALAYQRYFDRNVAL
                     VTGRLKDLPASQRVSLYHSMGNPLTTTGRPSLNQDWIDLAGGKNIAENWFGEHQQNRS
                     GEVALEKIVTANPAVIIAMNKRDADAILSSPQWASVDAVIHHRVYVNPKGMFWWCRET
                     SEEALQFLWLAKTLYPARFADVDIRKETREFYRQFFGLTLSDAQMSDVLNPPR"
     gene            complement(7133..9205)
                     /locus_tag="J1Z28_003680"
     CDS             complement(7133..9205)
                     /locus_tag="J1Z28_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7718478.1"
                     /translation="MIVTGNTAADTTDSAAGAGFKTNDIDVGPLGTKSWIETPYSSTT
                     VTKEMIENQQAQSVSEMLKYSPSTQMQARGGMDVGRPQSRGMQGSVVANSRLDGLNIV
                     STTAFPVEMLERMDVLNSLTGALYGPASPAGQFNFVAKRPTEETLRKVTLGYQSRSAF
                     TGHADLGGHFDENKRFGYRVNLLDQEGEGNVDDSTLRRKLVSVALDWNIQPGTQLQLD
                     ASHYEFIQKGYVGSFNYGPNVKLPSAPNPKDKNLALSTAGNDLTTDTISTRLIHYFND
                     DWSMNAGVGWQQADRAMRSVSSKILNNQGDISRSMKDSTAAGRFRVLSNTAGLNGHID
                     TGSIGHDLSLSTTGYVWSLYSAKGTGSSYSWGTTNMYHPDAIDEQGDGKIRTGGPRYR
                     SSVNTQQSVTLGDTVTFTPQWSAMFYLSQSWLQTKNYDKHGNQTNQVDENGLSPNAAL
                     MYKITPNTMAYVSYADSLEQGGTAPTDESVKNAGQTLNPYRSKQYEVGLKSDIGEMNL
                     GAALFRLERPFAYLDTDNVYKEQGNQVNNGLELTAAGNVWQGLNIYSGVTFLDPKLKD
                     TANASTSNKQVVGVPKVQANLLAEYSLPSIPEWVYSANVHYTGKRAANDTNTSYASSY
                     TTWDLGTRYTTKVSNVPTTFRVVVNNVFDKHYWASIFPSGTDGDNGSPSAFIGGGREV
                     RASVTFDF"
BASE COUNT         2286 a         2480 c         2345 g         2095 t
ORIGIN      
        1 tgttggtaat gcagtcatcc tgctggcaaa ggatgtgata tataacgagt acttttttga
       61 tctgcggaag tcatttttcg tgagcgttaa agatctgatg gcctctggca caaaaggtat
      121 tccggagaca cagaaatatc ctgacgttta ttgctggatc ccgcttttct ccataaatta
      181 cggcgtggtc attaccccgg tctataaaat tgatcccctg aaacccgtaa cggtaaaaaa
      241 accggataag atcactgttg tttgtaatta cagagaataa tctcagcctt ctccgctcac
      301 cgcaacagtg tttacctcgc tttatcttcc gttcagtcaa tacaaaccac cgctctggca
      361 ggtacagggc gtggtttctc tccggtactc ccctgcgcag taataatcat cactgaaaat
      421 actgattccg tcacgacaaa acgccatccc atattccggc aacacaaaat catcttccgt
      481 ttataccggc ttcactgaca aaacagacaa cctgtattgc ctgtacacaa ccgtttctct
      541 cctggcagga atattcccct actgattttt tacgtttctc cccggttgtt atgccggtga
      601 aggtggtgcg ttgttttcat catcaacgcc ggttgagatt aacaacatcc ggaggagatt
      661 tttatgacca ccccagtttc gctgatggat gaccagatgg tcgatatggc gtttatcact
      721 cagctgaccg gcctgaccga taagtggttt tacaagctca tcaaggatgg ggcctttcct
      781 gcgcccatca aactcggccg gagctcccgc tggctgaaaa gtgaagtgga agcctggcta
      841 caggcgcgta ttgcacagtc ccgtccgtaa tttttaacca ctgccccttc acccacagca
      901 gacgcaatct ccggcctgcc ggcggcgttc tgctgcctgt tatcccccgt aaggaatatg
      961 aaaatgaaac cacagtatca gacccgttac gagctgctcc acgaaagcta ccagaaatgg
     1021 ctgaccggct tcacccggca cgccgtatcc tggggcgtgt gtcatccgaa tatccactac
     1081 ttccataatc tgacgcccgg gtgggtgtca ttcaatggtg aaaaaccgga gattgccatc
     1141 gtcccccgga gtctgcaccg gctgatttat ggctctgaca aacgggccac gccgcccctg
     1201 gatgatgatc tggtggtgaa cttatgcacc agcgaacatc tgctggttca tcatccgatg
     1261 ctggaaggca ttctgctgtc tgaatgtgaa cgtctgaggc agcgttctct ggcaaataaa
     1321 ctgatcagcc tcttccgtca gtttggtggc actgagttgc gcctcaaact ggtctggctt
     1381 tgctggcttg atttaatgac cggaaacagc cttgacgact ggacggagaa cctgaaacgg
     1441 aaatcagaaa aagagctggg ggaatggatc atcgaccgtc agaagcagaa cgcagcactg
     1501 gcggatctga tggatcagta cgtgctcctg gcataccgca caacggttga cgataaccgt
     1561 aactgatgcc ggttgcttcc ggaaggtgcg gcaaaatacg ggaatgctgc tcccggcccc
     1621 gcatgctcac ctgcagagcg ggggcgtcgt catactgagc catgacggat gtcttaaggg
     1681 catcctgtca ctgctgagct ggcatgctgc ggtacatgcc tctcctggtg acaataagta
     1741 catggcgaca gcgctccctg aaccgcggaa gagatgtaca cggccggacg ccgtcagtgt
     1801 atacgggtag acgttccatg ctgcatgact acatccggcc cggatgtcaa tgagtccgtc
     1861 aggaacgctt cgcttcgaaa cccggcctgg ataccagcgc cggcatgccg ggaagaagtt
     1921 gatatggtgg ttgcagggca acagcaatcc tgaatcaact gctgcatcac tcaaccacgc
     1981 tgaatatcaa aggagagcgt gaccagttaa aagagaaacg caaatctgta gtgctgcaca
     2041 aaaacacaac atcagtcagt gaggatgaaa tggtgaaaag cagacagcat cagtattgaa
     2101 aatatcctgg ctggcatgaa aatggcccgg cccgtggcgt tgacatattc tctcctgagc
     2161 atcctgaatg actgcagtta ctgtggaaaa gtaagcagtc tccacccacg gcaccactta
     2221 acagacattc agtaactccc cccggacttt ctatgatgca ttatcggtgc actgaataca
     2281 ggagagtgcg cttaaaaccg cagaccgcat tgcgggtgtt actgcatgac tgcctggcgg
     2341 tattgtgaga tgtcggcagg aaccgatgtc tgcgcagggg gaacttccgt gatcgggata
     2401 agtgcagaag aaatcagatc acgacgtaaa cctgcagtgc ctgacgctgc aagtggcatt
     2461 aaccatggga cgttgccgat cccggattgc ggaaacagga gcactatttt ctgccgcggg
     2521 gagtacctct ggcagtaaat ccggctcgct catctgaccg tctctctccc ggaaaagacc
     2581 agaaatgaga tcgtcacctc aacagcaggg aagatgtctt gacagactgg ttatattgca
     2641 tacgatttat gaagaaaaag acagcaaata tggaggctat agcggagaac accctacgaa
     2701 attcctgcgg aaaacgattg aaaaaacgat cagattcgat tcgtttttat atgctttttg
     2761 ggcacgtggc gatgacgaac tgttgcatgt gcagaggaga atgcacccgg aagcaccaga
     2821 atcatccaga aaggcagaac gattacgcat caaaccagga gacagcaata tcaggataac
     2881 atactgattt aaaatgaatt tacagcaata cgtgaaaaca ttttttactt aaaggagaca
     2941 gatgcgctga agacagtgca ggtattttta tataaaaatg agaaggttac gggatgtcta
     3001 tccggcacac tatgaccgga gaatatcagt caggcttaaa aagacaataa atacagtgag
     3061 ttaaatacac agagaaaacg aaaacgtagt atcagagggt acatcttaca acagagactg
     3121 aatttccggt ttttcataac cggcacattc cggaaagagc aggctaaccg gaggctttct
     3181 gacaccagtc cctggcagtt taatctgtac atcatgtacg gatgttttgt tcgcattttt
     3241 aaaatcggca aaaatgcgag aagagcagct atattgtgga tgaactgaaa agtgccaggg
     3301 agcaacaaaa aaggcgtgat gagcgtgctg atggttccgt cctgctgaaa ctgtccggaa
     3361 aaatccacaa acagcttgaa agtattgccc gaagtcgtgg tgtcccccca aaaaaaatgc
     3421 tgaatgaaat gattgagcag gcgcaccggg attttgtggc aaatgagcag cataagaccc
     3481 ggagttaaag acggcagggt ccccggttac caattctgca gcgtactcaa caaatgtatc
     3541 cggcccttac ctgtgaggga cacgaaaatt atacattgag catgagtgga taatcccggg
     3601 tgagagtctc ccgtttttac actattcact aaaggtgagg catatcattc agtctctctg
     3661 aattcttcat ttcagtaact tcagctaccg tcgtttcatc tctcccacga accggagccg
     3721 caacttgttg catcacaggg ctgacaagtg aactaaaatc cgcaccttaa tattctgact
     3781 ggctgcgtac attcattgca tgaagcggcc ggagcggctg agctgaaggt ccgttgtgag
     3841 cgaacagcgg acattcacta cacatggttc aaacatgaac cggagcttta ttgtctagct
     3901 gtcagtgtac tgcgatttac ccgctcggtt gtccagaaaa tcggtctgaa atccagtcat
     3961 taaagggcct tagcctgaca ttaagcaact tggtaggcgg atagataata gaaataggaa
     4021 tgcgcaggtc tgtcatgttg cctagaacgg tgacaagctg tccggtgcga aatcattccg
     4081 gcagcgacag gctcatgtgt cagcccgacg cccagaccgt caacgagcat gtggatctgc
     4141 gaggcgttat cgttcgcagc gatgttaaaa cgaacaatct gctcatcttc tagaagcaca
     4201 agtgctttaa tgacattaag gcggtctata tgttgcatta cagacttcct attgatcttt
     4261 acctgaggct gaagcaacca cgttaatccg actatttttc gattgcacca tccactatca
     4321 ccatcgatct tccctgagca caacgttcaa ctctcccgcg tacgccgtat acctgcgcaa
     4381 ggctctctgc atggatcacc gtttgcgggt cgccgctgtc gataagtttc ccctctttca
     4441 gcatgatgac ctgggcggca tgccgcagtg cgatattgat atcgtgtaag acgaccagcg
     4501 taaccatatt gcgtcttcgc gtttcgcgcg atacaacatc catcacatgg aactgataat
     4561 tgagatccag cgcgctcagt ggttcatcca gcaataataa tgcggggcgg cgaataagcg
     4621 actgtgcaag ccccaccagc tgcttctgac cgccggacag gctatcgagg tagttcattg
     4681 ccagatgtgc gatgcctagc tcttcgagca gcgctatagc ctgcgcctga ttttgcccgg
     4741 cgccggaggc gcgctgggcg acaaccaccg actccagcac ctgtaaatgc acgccctgcg
     4801 gcagggactg cggcaggaac acaacttttt cagcccgacg agcaaacggc agcgataaga
     4861 gattctcttc attcagccag gcttcgccac tggcgcgatt tagcccggcc agggcgcgca
     4921 gtagcgttga tttaccgcag ccattaggcc ccagcagtac agtgacttcg ccgcgcggca
     4981 gcgtagaaat cgacagatgc tcaataatcc gccgtttgcc gtaaccggcg cacagcgcat
     5041 taatcgtaag cccgctcatg acatactccc tcgatgacgc attacaatgc tcagaaagaa
     5101 cggaacgcct accagcgagg taacaattcc taccggtaaa atgacaccgg gaatgatgtt
     5161 cttcgacgct accgaggcca gcgatagcac cagcccaccg ataaggatgc ttccgggcaa
     5221 ataaaagcga tgatcttctc ccagcaacag gcgggaaata tgcggtgcaa ccaggccaat
     5281 aaagccaatt ggccccacaa acgcaaccga aagggccgcc agcaggctga tgcgcagcag
     5341 ggaacccagg cgcaaacggc gaacatcaat gccaaaactc atcgcccgct cttcgccgag
     5401 gcgtagcgcg gtaagctgcc aggcacgacg cagcgaccag ggtaaaacaa tcgccatcgc
     5461 cgccgccagc accgccagtt tctcccatga tgcgcgagcc aggctgccca tcgtccagaa
     5521 caccaggccc tgcaaagtat cttcatcggc gacaaactgc ataatggaca ccagcgcatt
     5581 gaaggtaaac accagcgcaa tcccaaacag cacaacgccc gacgtcggca cgcgcgtcca
     5641 gcgggtcaga ctatcgagca gcagggcgga caggagcgca aaaagaaaag cgtttgccgg
     5701 tataaacccg ctttcgggaa cgccgggaat accgatgccc agaacaatcg ccagcgctgc
     5761 gccaaaggcc gccgccgacg aaacgccaag cgtaaacggg ctggcgaggg agttattcag
     5821 aatagtttgc atctctgccc cggccaaacc cagcgccatt cctaccagga gcgccatcag
     5881 cgcatagggc aaacggatgt cccaaacgat aacccgcatg ccgttagtcg ccccggacgg
     5941 atgcatcaaa gtctttatca gcgaatgtaa agggagccct gaggggccga gggtgaaatc
     6001 gagcagcagc gaagcaatga tagcgataaa aataaccgcc agtattacca gacgctggcg
     6061 aaaaagctgg cgatagcggt tctcgaccga cagcgtactt tccaatacag tcatggtttt
     6121 tcacagcctc cgttagcgcg gcggattgag cacatcgctc atctgcgcat cgcttaacgt
     6181 caggccaaag aactgccgat aaaactcacg cgtctctttg cgtatatcca catcagcaaa
     6241 tcgcgcagga taaagcgttt tcgccagcca caaaaattgc agcgcctctt cgctggtttc
     6301 acggcaccac cagaacattc ctttcggatt aacgtaaacg cgatgatgaa tgaccgcgtc
     6361 cacgcttgcc cactgcggag aactcagaat ggcatccgcg tcgcgcttgt tcatcgcgat
     6421 gataaccgcc ggattggcag tgacaatttt ctccagcgcg acttcacccg aacgattttg
     6481 ttgatgctcg ccaaaccaat tttcagcgat atttttgccg ccggccaggt caatccagtc
     6541 ctggtttagc gagggtctac cggtggtggt cagcggattg cccatactgt gatagagcga
     6601 cacgcgctgt gacgcaggaa gatctttcag gcgcccggtg accagggcca cgttgcgatc
     6661 gaagtagcgt tgataggcga gcgctttttg ctgcgcatcg ggccccagca ccgctgccgt
     6721 ttgctgtacg cgctgcgtta acgcccgcag tgaattcgcc tcaaacgcca gcacctgcac
     6781 cccagcctgt ttcagcaacg cctgttgagg cactaccata ttctgcggta caaaaagcat
     6841 atcgaccccc agcgcgataa tctgttcggg attgatttca tgcccgctgt ttacgctaac
     6901 tgctgcgact ttctctatgc gcggcaggct ctggcgaaac agggggatcc tttttgcaat
     6961 catcgaggtt ccgacaatgt tatcgccgta ccccagcatg gcaataatgg tattttgcgc
     7021 cggccacggt gacgcgatac gcacctcctt gtgcgtattt tctgcgtgca ggctggccgc
     7081 cagcagcagc gccagcagcg gaaataaacg ggagagtttc atcatttatc catcagaaat
     7141 cgaaggtgac ggatgcacgc acttcgcggc cgccgccgat aaacgcactt ggggaaccgt
     7201 tatcgccatc ggtacccgat gggaagatag aagcccagta atgtttatca aacacgttgt
     7261 ttaccaccac gcggaaagtg gttgggacgt tgctcacttt cgtggtgtaa cgcgttccca
     7321 aatcccatgt ggtatagctg ctggcgtaag aggtgttggt atcgttcgcc gcgcgtttgc
     7381 ccgtataatg gacgttagcg ctgtaaaccc attccggtat ggacggcaaa ctgtattccg
     7441 ccaacagatt ggcctgcact ttcggcacgc cgacaacctg tttattgctg gttgaggcat
     7501 tcgccgtatc tttcagtttc gggtcgagga aggtcacgcc gctgtaaata ttcagcccct
     7561 gccacacatt cccggcagcg gttaactcaa ggccgttgtt aacctggtta ccctgctctt
     7621 tatacacgtt atccgtatca agataggcaa acggacgttc cagtcggaac agcgcggcgc
     7681 ctagattcat ctcgccgatg tccgatttta gccccacttc atactgcttg ctgcgatacg
     7741 ggtttagcgt ttgaccggca ttttttacgc tctcatccgt cggtgcggta ccgccctgct
     7801 ccagcgaatc ggcatagcta acgtaggcca ttgtgttagg ggtaatttta tacatcagcg
     7861 cggcgttcgg acttaaacca ttttcatcaa cctggttcgt ttgattaccg tgcttatcgt
     7921 agtttttagt ctgcagccag ctctggctga gatagaacat tgccgaccac tgcggcgtaa
     7981 atgtcaccgt atcgccgagc gtaacgctct gctgagtatt tacgctggag cggtatcgcg
     8041 gcccgccggt gcggattttg ccatcgccct gctcatctat cgcatccggg tgatacatat
     8101 ttgttgtacc ccagctatag ctggaacctg ttcctttggc actataaagc gaccagacat
     8161 atcccgtggt agaaagtgac agatcgtggc cgatagagcc ggtatcaata tgaccattca
     8221 gcccggcggt gttgctcagg acgcgaaaac gtccggcagc ggtggaatcc ttcatcgaac
     8281 gagagatatc gccctgattg ttgagtattt tactggaaac actacgcatc gcgcggtcag
     8341 cctgctgcca gcccacgcca gcgttcatgg accagtcgtc gttaaagtag tggatcaggc
     8401 gagtgctgat ggtatcggta gtgaggtcgt tgcccgcagt gctgagcgcc agatttttgt
     8461 ccttcggatt cggcgcagac ggcagtttga cgttcggccc atagttaaag ctaccgacat
     8521 agcctttctg gataaattcg taatggctgg cgtcgagctg tagctgagtg cccggctgaa
     8581 tattccagtc gagcgcaacg gaaacgagtt tgcgacgcag cgtgctgtca tccacattgc
     8641 cttccccttc ctgatcaagc aggttcacgc gatagccaaa ccgtttgttt tcatcaaaat
     8701 ggccacccag atcggcatgg ccggtaaacg cactgcggct ttgatagccc agcgtcactt
     8761 tacgcagcgt ctcttcggtt gggcgcttcg ccacgaaatt aaactgccct gctgggctcg
     8821 ccgggccgta cagcgcgccg gtcaaactgt taagcacatc catgcgctca agcatttcca
     8881 ccggaaacgc ggttgttgaa acgatattca gcccgtccag acggctgttg gccaccacgc
     8941 tgccctgcat cccccgactt tgcggacgcc cgacatccat tccaccgcgc gcctgcattt
     9001 gcgtactggg agagtatttc agcatctcgc tgacgctttg cgcctgctga ttttcaatca
     9061 tctctttagt tacagtggtg ctggaatatg gtgtttcgat ccaggatttc gttcccagcg
     9121 ggccgacatc tatatcgttc gttttgaaac cggcaccggc ggcagaatcg gtggtgtcgg
     9181 cggcggtgtt gcccgtgaca atcatt
//
LOCUS       DADSZD010000166         9201 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.167, whole genome shotgun sequence.
ACCESSION   DADSZD010000166 DADSZD010000000
VERSION     DADSZD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.167"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(163..954)
                     /gene="cpoB"
                     /locus_tag="J1Z28_003681"
     CDS             complement(163..954)
                     /gene="cpoB"
                     /locus_tag="J1Z28_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBA7718479.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(964..1485)
                     /gene="pal"
                     /locus_tag="J1Z28_003682"
     CDS             complement(964..1485)
                     /gene="pal"
                     /locus_tag="J1Z28_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBA7718480.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="J1Z28_003683"
     CDS             complement(1520..2812)
                     /gene="tolB"
                     /locus_tag="J1Z28_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBA7718481.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYIMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(2945..4210)
                     /gene="tolA"
                     /locus_tag="J1Z28_003684"
     CDS             complement(2945..4210)
                     /gene="tolA"
                     /locus_tag="J1Z28_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBA7718482.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQELSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(4275..4703)
                     /gene="tolR"
                     /locus_tag="J1Z28_003685"
     CDS             complement(4275..4703)
                     /gene="tolR"
                     /locus_tag="J1Z28_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBA7718483.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(4707..5399)
                     /gene="tolQ"
                     /locus_tag="J1Z28_003686"
     CDS             complement(4707..5399)
                     /gene="tolQ"
                     /locus_tag="J1Z28_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBA7718484.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5396..5800)
                     /gene="ybgC"
                     /locus_tag="J1Z28_003687"
     CDS             complement(5396..5800)
                     /gene="ybgC"
                     /locus_tag="J1Z28_003687"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBA7718485.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(5950..6243)
                     /gene="ybgE"
                     /locus_tag="J1Z28_003688"
     CDS             complement(5950..6243)
                     /gene="ybgE"
                     /locus_tag="J1Z28_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBA7718486.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6243..6356)
                     /gene="cydX"
                     /locus_tag="J1Z28_003689"
     CDS             complement(6243..6356)
                     /gene="cydX"
                     /locus_tag="J1Z28_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBA7718487.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6371..7510)
                     /gene="cydB"
                     /locus_tag="J1Z28_003690"
     CDS             complement(6371..7510)
                     /gene="cydB"
                     /locus_tag="J1Z28_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBA7718488.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(7526..9094)
                     /gene="cydA"
                     /locus_tag="J1Z28_003691"
     CDS             complement(7526..9094)
                     /gene="cydA"
                     /locus_tag="J1Z28_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBA7718489.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2243 a         2521 c         2201 g         2236 t
ORIGIN      
        1 tttttcgttg ctttcggttt gtttgatatc ccgtggcaac ggcggcatat attactgatt
       61 tcagacttga gcgcaacaaa aatttcgatg tagatcactc aactgcttat gattcgcacg
      121 acacgaccag aaataatgcg acttctggtc gtgtgttatg cattacatcg cgttcagacg
      181 tttttgcgcc tgtttagcgc catcagtacc agggtattta ctgataacct gctggtatac
      241 ggctttcgct tttgcggtgt cacctttgtc ctgcatgatg acgccgactt taaacatcgc
      301 atctgcagcc ttcggtgact tcggatagtt tttcactacc gaagcaaaat aatacgccgc
      361 atcatctttt ttacccttgt tgtagtttaa ctgacccagc caataattgg cgtttggcag
      421 gtaagttgaa tcagggtaat ttttgatgaa attctgaaat gccaccattg cgtcatcctg
      481 gcgggattta tcctgcacca gcgcaatagc tgcattgtaa tccgtgtttg catcaccgct
      541 tttcaccggc gcgccagcat tcgcagtacc agcatcagct gtcggcgtcg ttgatgccgc
      601 cacaccgctt tgatcgccgc tggttgattg cgccgctgca ccaccgctgc tgaggctgtc
      661 gatctgcaac aggatctgct tttgccgctc cacgacctga ttcagttgat actgattttc
      721 ctgaatctga ccacgcaggg aatcaatatc ggattgatta tcagagagtt gttgctggag
      781 ttgggttaaa agctggctgt gagcattaga aatacgctca agttgagtga cgcggtcttc
      841 gaccgagcct gagccgacac tactgattgg tgcctgagca aaagcggccc agggggccgc
      901 tataccaacc agtaacgaca gactcaatag ttgatgtctg aagttactgc tcatgcaatt
      961 ctcttagtaa accagtaccg cacgacggtt tttggcatac gccgcttcgt catgacccag
     1021 tactgcaggt ttttctttac cgtaagaaac gatggagatc tggtcagcag aaacgccttt
     1081 accctgcaga tacatcttaa cagcgttcgc acgacgttca cccagggaga tgttgtattc
     1141 cggagtacca cgttcgtccg cgtgaccttc tacggtgact ttgtaagacg ggttgctacg
     1201 caggaagttt gcgtgtgcat ccagcatttg agcgaagtca gaacggatat cgtacttgtc
     1261 cagatcgaag taaacgatgt tgttctgctg cagctgttgc atttgcagac gagcctgctc
     1321 ttcggaagac atgttgccgt tgccgccgtt cgcatccata ccagtgccgg cacccagcat
     1381 gccttcgctg ccgtcattgc tggcgttctt gttggaagaa catgccgcaa ttgccataac
     1441 aggcagagca atcatcagcc ctttcagcac tttgttcagt tgcatttcaa tgattccttt
     1501 actattcaat taattattat cacagatacg gcgaccaggc agggaatttg acctgtccat
     1561 cagttgccgg aagacgcgct ttgaaacgcc catctgtaga aaccaaattc agcacggatc
     1621 ccatcccctg agaagagctg tagattacca tagtgccgtt aggtgccaga cttggcgttt
     1681 catccaggaa cgtggacgac agaacttgta cgcctcccgt tgccagatct tgtttggcaa
     1741 tgtgctgctg cccaccgttg gagctgacca ttaccataaa tttaccgtcg ctgctgacat
     1801 ccgcatcctg gttctgcgaa ccttcccagg taatacgttg tggcgcaccg ccgttgatat
     1861 tcactttata aacctgcgga cgaccggcct ggtcagaagt aaatgccagg ttctggctgt
     1921 ccgggaacca ggtcggttcg gtattgttac tgcgaccatc agttacctgg cggatctgac
     1981 cagaagccaa atccattatg tacaggttca ggctaccggt tttcgacaag gcgaatgcca
     2041 gtttgctgcc gtctggcgag aatgcaggtg caccgttgtg acgcgggaat gaagccacct
     2101 gacgtacagc gccatttgcc agcgtctgaa taaccagcgc ggaacgaccg ctttcgaagg
     2161 tcacataagc cagtttagaa ccgtctggtg accacgccgg agacatcagc ggttgcggtg
     2221 aacggtgaac gacaaactgg ttgtaaccgt catagtcaga tacgcgcagt tcatacggga
     2281 actgaccgcc gttagtctga acaacgtagg caatacgggt acggaacgca cctttaatac
     2341 cggtcagttt ttcaaatact tcgtcactgg cggtatgacc agcataacgc agccactgct
     2401 tgttcacttt gtacgagttc tgagcaagta cagtacccgg tgcgccgcca gtgtcaacaa
     2461 gttgataagc gacattgtaa gaaccatccg gattcggagt gacctgaccg acaaccacag
     2521 cgtcaatgcc cagcgcggac catgcagctg gttgtacttc ctgcgcacta cccggctgct
     2581 gtggcagacg agcacgatct aacggattaa atttaccgct gttacgcaag tcagcagcaa
     2641 cgatgccgcc aatatcttca ggtgccgcac caggccccgc ccactggaaa ggaacaacac
     2701 caataggacg accggaatct acaccgctgt cgatcacaat gcggacttca gcatgcagaa
     2761 ctgatgccca cagtatgaga aaaccaaatg ctactcgtaa tgcctgcttc atcatatctc
     2821 ccttatctgg accgatggcc cacgataatt tagcagaatg ttaacaaact aaaatacaca
     2881 aaactaccag aaccccgtgg caaccggtgc ctgatgttga ccgtcggaac agtcaacatc
     2941 gcgattacgg tttgaagtcc aatggcgcgt ttttgaacac ttcatatact gcctggcttg
     3001 gtggtttcgg gatcttcgca agtttagctg ctgccaacgc agcttgacaa agtgcgggat
     3061 cgccaccttc aggtttgata tccagtaaca taccatcggg tgccagtttt atgcgcagcg
     3121 tacaggtttt gcctgcatag gacgatgcgt catagaactt actttcgata gcagatttaa
     3181 tctgcccggc atagttattg atatcggccc ctgatgcgcc attgttttta gtattaccac
     3241 tcccggcagg cgaagcattg ttccctttcg cccctccccc agttttcggt gcattcttac
     3301 cagagcttag ctcaccgaaa atatcatctg cctcagcggc agcttttgct gcagcggctt
     3361 tttcagctgc tgcttttgcc gctgccgctt ttttgtctgc ggcggctttt tctgccgctg
     3421 ctttcttatc agctgctgcc ttttcagcag ccgctttttc tgctgctttt ttgtcggctg
     3481 ccgctttctc tgctgccgct ttcttatcag ctgcagcctt ttcagcagcc gctttcttct
     3541 cagcttctgc cttggctttt tcagcagctt cggttgccgc tttctttctt gcttcagctg
     3601 cagctgcttc tgccgcttca gctttcttct tcagcgccgc agcagcagcc tcggcttttt
     3661 tctgcgcttc gactgcggct ttggcggctt ctgcttctgc ttttttcttc gcgtctgccg
     3721 ccgctttctt cgctgcttct tccgcagctt tagcatccgc ttcggccttc gctttagcat
     3781 ctgccgccgc tttcgctgcc gcctcttccg cttgcttctg ctttaactcg gcctgttttg
     3841 cggcttcttc agcctgcttt ttctgctcct gagccgctaa ccgctctttc tcaagttgct
     3901 tcaggcgttc ctgttcagcc gcttgtttct cacgcagttc ttcagcagcc tgctgctcct
     3961 tcatcttgcg ctgctcatca gaacgcttcg cgcttaattc ctggctttgc atacgtttgt
     4021 actgctcaac taccgcacct gaatcaacca tgacagcgtc gatggacgaa ccaccgccgc
     4081 ctccggctga agcttctata ttctcatcga acgaactcca gatcagcgct gcaaataaga
     4141 tgacatgcag cactgctgaa ataattatcg cccgcttgag cttgtcgttt tgttcggttg
     4201 cctttgacac tctcggtttc caaaaactgt tcgcctgtta cccgctctct ttcaagcaag
     4261 ggaaacgcag atgtttagat aggctgcgtc attaaaccaa ccgatttcac acccgcacta
     4321 tgtaacaagt tcagtgcttt aattatttca tcgtaaggca catcttttgc gccaccgatc
     4381 agaaagaccg ttttcgggtt ggccttgaaa cggctggaca cttccgccac cacctgctct
     4441 ggtggtaaac gctccagacg atctttctca accaccacgg tgtactgacc aataccagac
     4501 acttcaacaa tcactggcgg attatcgtta ctgctcaccg cctgtgattc agtagcgtct
     4561 ggcagatcga cctccacgct ctgggtgatg atgggcgctg tcgccataaa gatcagcaac
     4621 agcaccagca gtacgtccag caacggtaca atgttgattt cggacttgag atcgcgacga
     4681 cctcgtccac gcgctctggc catggcttac cccttgttgc tctcgctaac ggtaaacgcc
     4741 tggcggtgca gaatcgcggt aaactcttcc ataaagttgt cgtaattcag ttccagtttg
     4801 tttacgcgct ggtttaggcg gttataagcc attaccgccg gaattgcggc gaacaaacca
     4861 attgccgtcg caatcaacgc ttctgcgata ccgggcgcaa ccatttgcag tgttgcttgt
     4921 tttaccgccc cgagggcgat aaaggcgtgc atgatccccc agaccgtacc aaacagacca
     4981 atatacgggc tgatggagcc aaccgtaccg aggaaaggaa tgtgcgtttc cagattttca
     5041 agttcacgat tcatggaaat acgcatcgca cgcgacgccc cttccactac ggcttccggc
     5101 gcatggctat tggcacgatg caggcgcaca aactctttga agccgctgta aaagatttgt
     5161 tccgaaccgc tcagattatc ccgtttcccc tggctctctt gatagaggcg agagagttcg
     5221 attccagacc agaatttatc ttcaaacgct tcggcttcgc gcgccgctgc gttaagaata
     5281 cgggtccgct ggataataat ggcccaggat gcgattgaaa aaccaatcaa aatcaacatg
     5341 ataagtttaa ccagaaggct agccttcagg aacaaatcaa ggatattcat gtcagtcact
     5401 gcttaaactc cgcgacaata gacttgggaa gcgcacgagg cttcattttg agtgggtcaa
     5461 cgcaaacaac cagaacctct gcttcattca acaaagtatt ctcggcgttg acaatacgtt
     5521 gcgtgaaaac caaagaggtg ccacgcattg atgttatttc agtctgtatt tcgagcatat
     5581 cgtcgagccg cgcaggtgcg taatattcca ccgtcatttt acgtaccaca aaggcaacgc
     5641 gttcagccat cagcgcctgt tgactgaaat gatgatgacg cagcatctct gtgcgtgctc
     5701 tttcataaaa agcgacgtaa ctggcgtggt acaccacacc accggcatcg gtatcttcat
     5761 agtagacacg aaccggccat cgaaacagcg ttgtattcac tttacatccc ggtaatgcaa
     5821 caaaagttag agcttttaaa cgctgctact atacgcgcgc gattgaggtt tgggaatgag
     5881 ggcaagttaa gggagcgtaa aaatttatgg gcctgactcg gcccataaat ttttaaagag
     5941 aattctgatt taaaagaaga agaaaatcag cccgacaatg agaacaatat cggcaagcaa
     6001 tgggcaaaaa atcccttgcc agagaacctt ctgcggacga aagcccacgc cgtgaatcac
     6061 accagcacag acggcccaca taagcagtaa accatgccag atttccagtt cactggtctt
     6121 cgcggcgaaa cgtgatgggt cccaaaacat acatcctgct aacagaagtg ccatcacgaa
     6181 ggaaagcgcc cgcagggggc gcttgtccat taccgcatac aaggttgcga taatcttact
     6241 catcagatgt cttcttgacc ggctttgcct gattcgacgt gctcaagcgc cagcgcggtg
     6301 attaccccaa acgaacaggc aagaagcgtt cccagaatcc atgcgaaata ccacattttt
     6361 agctccttac ttagtacaga gagtgggtgt tacgttcaat atcttctttg gtgatacgac
     6421 cgaacatttt ccagtaacac caggcggtgt agagcagaat gatcggtacc agaaccaccg
     6481 caacccaggt catgacgtta agcgtcagct ggctggaagt tgcatcccac attgtcagac
     6541 ttgcgttcat catggtgctg gacggcatca caaacgggaa cattgcgata ccggcagtca
     6601 ggatgatgca ggccagcgtc agggaggaga acacaaacgc ccacgcggct ttatccatac
     6661 gtgcagtcag gatggtcagc agcggcagaa ccacacccag tgccggaata gcccacagaa
     6721 ttggcgtgtt gttgaagtta accagccatg cgccagcttc acgaaccact tctttattca
     6781 gcgggttaga ggctgcgtaa tggtccattg tcgatttcac gacataacca tcgataccgt
     6841 acatcaccca tacgccagcc agtgcgaagc agaccagcgt caccagtgcc gccacctgag
     6901 ccgttgcacg ggtacgcagg tgcagttcgc ccacggtacg catttgcaga taggttgcgc
     6961 cctgagtaat gatcatccct acgctcacca cacctgccag caggccgaac gggttaagca
     7021 actggaagaa gttaccggtg tagtacagac gcagatattc atcaacgttg aatggtacgc
     7081 cctgcaacag gttaccgaac gctacaccaa tcaccagcgg cggaacgaag ctaccaatga
     7141 agatgcccca gtcccacatg ttacgccagc gggtttcttc aatcttagag cggtagtcaa
     7201 aaccgaccgg acggaagaac aaagacgcca gtacgaggat catcgccaca tagaagccgg
     7261 agaacgcagc ggcatagacc atcggccagg cagcaaacag tgcgccgcct gcggtgatca
     7321 gccaaacctg gttaccgtcc cagtgtggtg cgatggagtt aatcataatt cgacgctcgg
     7381 tgtcgttacg accgaggaaa cgggtgagca tgcccacccc catgtcgaaa ccgtcagtga
     7441 ctgcaaaacc aattagcaga acgccaacca gcagccacca gataaaacgc aatacttcat
     7501 aatcgatcat ttgacgactc ctgtcttagc gtgccggctg agtagtcgtg gaagactgct
     7561 caaagtgata gcgaccggtt ttcaggctgc ttgggccgag gcgtgcaaac ttgaacatca
     7621 agaacaattc tgccaccagg aacagggtat acaggccgca aatcagcacc atcgagaaga
     7681 tgagatcgcc tgcggtcagt gacgagttcg ccacagctgt cggcagcact tcaccgatag
     7741 cccacggttg gcggccatat tcagccacga accagcccgc ttctacagca atccacggca
     7801 gcggaatacc gtacagcgcg gcgcgcagca gccatttttt ctcgccaatg cggtttcgga
     7861 tgacactcca gaaagagagc gcgatgattg ccagcagcag gaagccacac gccaccatga
     7921 tacggaacgc aaagtacagc ggcgctacac gcggaatgga gtctttggtt gcctgttgaa
     7981 tctgagcttc agtcgcatca gccacgtttg gcgtatagcg tttcagcagc agaccgtaac
     8041 cgagatcttt cttcatgcta ttgaactggt cacgaacggc ctggtcagta gaaccagaac
     8101 gcagttgttc gagcagagag tacgccttca tcccgttacg aatgcgttct tcatgctgca
     8161 ccatcagttc tttcagaccg ataactggag tatccacgga acgcgttgca atgatgccca
     8221 gcgcgtaagg gatctggatc gcaaatttgt tcgtttcctc ttcctgatca ggaatgccga
     8281 acagagtaaa ggcagcaggt gcaggttgcg tttcccactc ggcttcgata gcagccagtt
     8341 tggttttctg cacgtcgccc atttcgtagc cggattcatc acccagaaca ataacagaca
     8401 gaacggcagc cataccgaag ctggcagcga tagcaaagga gcgtttagcg aaggcgaagt
     8461 cacgaccttt cagcatatac catgcgctga taccgaggat gaacattgcg ccagtcacat
     8521 aaccagaagc tacggtgtga acgaatttca cctgagcaac cgggttaagc accagctcgg
     8581 agaagctcac catctccata cgcatagttt caaagttgaa atcggacgcg attgggtttt
     8641 gcatccagcc gtttgcaacc agaatccaca gtgcggacag gtttgaaccg agcgccacca
     8701 gccaggtgac acacatatgc tgaactttac ccagacgatc ccaaccgaag aagaacagac
     8761 ctacaaaggt ggattcgagg aagaaggcca tcagaccttc gattgccagc ggcgcaccga
     8821 agatatcccc tacatagtgg gaatagtaag accagttagt cccgaactgg aactccatgg
     8881 tcagaccggt agccacaccc agagcaaagt tgataccaaa caacttgccc cagaacttgg
     8941 tcatatcttt ataaatctgt ttgccggaaa ggacgtagac cgtttccata atggccagca
     9001 ggaacgccat accgagcgtc agtggcacaa aaaggaagtg gtacatcgcg gtcaaggcaa
     9061 actgtaagcg cgacagttcg actatatcta acatcatgac tccttgctca tcgcatgaag
     9121 actccgagag tgaaccccgt tagaaagggt cacacgcatg ccccaataca aaattatttg
     9181 ctcgcctcta tcgttgccat t
//
LOCUS       DADSZD010000167         8873 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.168, whole genome shotgun sequence.
ACCESSION   DADSZD010000167 DADSZD010000000
VERSION     DADSZD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.168"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(154..597)
                     /locus_tag="J1Z28_003692"
     CDS             complement(154..597)
                     /locus_tag="J1Z28_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HBA7718490.1"
                     /translation="MAELQKVDDWLSALLANLEPATRSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLRTAASSDSVSVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDEAEMITRDIFFRYLS"
     gene            complement(590..1021)
                     /locus_tag="J1Z28_003693"
     CDS             complement(590..1021)
                     /locus_tag="J1Z28_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718491.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAVAEPDEPEEMWTVKRG"
     gene            complement(1117..1545)
                     /locus_tag="J1Z28_003694"
     CDS             complement(1117..1545)
                     /locus_tag="J1Z28_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HBA7718492.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELRSKSQAL
                     AKSNSQLISLSILTETNNREQARLYADAEQTSVLLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGAGSAAH"
     gene            complement(1542..1919)
                     /locus_tag="J1Z28_003695"
     CDS             complement(1542..1919)
                     /locus_tag="J1Z28_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718493.1"
                     /translation="MKKKVISGLFLMLWIALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVSLAALIKNGPLWQELRASLLKTITRKENVFISWMMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(1921..2433)
                     /locus_tag="J1Z28_003696"
     CDS             complement(1921..2433)
                     /locus_tag="J1Z28_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA7718494.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(2414..2629)
                     /locus_tag="J1Z28_003697"
     CDS             complement(2414..2629)
                     /locus_tag="J1Z28_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718495.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(2633..2836)
                     /locus_tag="J1Z28_003698"
     CDS             complement(2633..2836)
                     /locus_tag="J1Z28_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718496.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVDLPDIPTTTTVQTVQLWD"
     gene            complement(2836..3300)
                     /locus_tag="J1Z28_003699"
     CDS             complement(2836..3300)
                     /locus_tag="J1Z28_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HBA7718497.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(3396..4046)
                     /locus_tag="J1Z28_003700"
     CDS             complement(3396..4046)
                     /locus_tag="J1Z28_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HBA7718498.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHALGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASAINHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(4050..5108)
                     /locus_tag="J1Z28_003701"
     CDS             complement(4050..5108)
                     /locus_tag="J1Z28_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HBA7718499.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAVAAETGA"
     gene            complement(5125..5958)
                     /locus_tag="J1Z28_003702"
     CDS             complement(5125..5958)
                     /locus_tag="J1Z28_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HBA7718500.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEVQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            6101..7867
                     /locus_tag="J1Z28_003703"
     CDS             6101..7867
                     /locus_tag="J1Z28_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HBA7718501.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            7867..>8873
                     /locus_tag="J1Z28_003704"
     CDS             7867..>8873
                     /locus_tag="J1Z28_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBA7718502.1"
                     /translation="MGKSKKNRAASTNQIQHKSQTTAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASRVFVRNELIPLQKRFEELNSWANEQVI
                     R"
BASE COUNT         1937 a         2614 c         2333 g         1989 t
ORIGIN      
        1 agagatcgag ctatggtgat ggaattacta cctcaaacaa aggaaaatat aaaaatagcc
       61 aatgagtgga gttctacaat tcatcagctt tactgtgact ttatctccac atgtaggaat
      121 aacttagcca tataaattat atgctgtagt taattaagac aagtaacgaa aaaaaatatc
      181 acgagttatc atttccgctt cgtcgttaac acccaaaagg cgacgctctg cataacggac
      241 ctcaggtcct ttgcggctga cgcgatcacg cagtccgtag tgatgaacac gggcaatgcg
      301 ctgtacctta ccttcaaact gtacgctgac agaatcagag ctggcggcag ttctcaggta
      361 ttttgtggtg cgcagctttg caaacatctg acgtttgatg cgccccttct tgctgcgtgc
      421 tgttacccta cgcggttcat aactgctgcc atctggattg cgctgcatcc tgatattctg
      481 ctgctgtgtc cggcgcagtt cctgcgccag ctggcgcatc atgcgacttc tcgtggctgg
      541 ttccagattc gccagcaagg cactaagcca gtcgtccacc ttctgcagtt cagccacgtt
      601 tcaccgtcca catttcttca ggttcatcgg gttctgctac cgcttcaacg ctcgacacac
      661 tgccgtcagt gctgaccagc acacgttccg tcagttgcag gttaaggctg atatcacaga
      721 catcgttgcg cagaatatcc acctcaaagg tgaatagctt ttcccgcaac gccgggttat
      781 tgatggcatc gggctggtta tcccgcagcc acagtaaaac cggggccatc agcagattct
      841 ggtcgccgct gaaatcctct atcaccacgt tcagggtata gcggtactcc catgacatgg
      901 agctggcccc cgtggcaacc agcgaaccgt tatccacaaa cagatgcagt ttgtccgggt
      961 tattgcggac ataaggcact gctttattga gggcgtggcg cagggattgt ggtttgttca
     1021 ctgtttcgct cctgacacgc aataatcatg tccactttgt ctgcacagac cgcccaggcg
     1081 gcctccgttt catccagcaa cgcgttcagg tcaccgttag tgcgcggcgc tgcctgcccc
     1141 agccgacacg gcgtcactcg cggacaacca ctgacggtaa gctgcacctc cggtgagtgt
     1201 ggggcgttcc cgcagccgga taatgtcagc aggcaaagga gtatcagccc agcggcgtaa
     1261 atcctcgttc tcacgtttca gttcctcaat ccggtgttgt cgttgtctca gtagtacgct
     1321 ggtctgttct gcgtcggcat agagtcgcgc ctgctcccgg ttgttggttt ctgtcagaat
     1381 ggacaggctg ataagctggc tgttgctctt tgccagtgcc tggcttttgc tccgaagctc
     1441 gtctgcctgc gtgctgatgg tctggctggc atcagccagc cgccacgtct gccagcccag
     1501 cgccgccagt aataacgcca gcacaaccag cagcaaccgg ttcatgctgc tacctgttgc
     1561 gccatctgat tacgggtgat ccagaaggca ataacggtca gtagataaaa gaccagggta
     1621 atagcccacc ccgtccaggc gagactgacg acaatcagca atcgcatcat ccagctgata
     1681 aatacgtttt cttttcgggt aattgtcttc agcaaagatg ccctcaactc ctgccagagc
     1741 gggccgttct taattaacgc agccagtgat accggaatta ctgcccatgt cagcagacag
     1801 gctacccaga caccagatgc tgccagtacc ggaaaaatcc cctgcggata caccattgcg
     1861 gcgattaaca gcgctatcca taacatcaga aacagcccgc tgattacttt ctttttcatt
     1921 tcagtttgct ccctgtaagc accaggccat ctcccgcgca cggcggttat ccagcccctg
     1981 attaaacaca ccttttacat acacccagcg cggcaactgt cggcacgcat ccgcccagcg
     2041 ccgctgattg agcaatttca ccagcgtgga actgcaggca ttgcccgtac ccacgttgaa
     2101 ggcaaacgac accgcagcgt catacacctt ctgcggcggc tgttgcttca cacacctttc
     2161 cagcgcccgc tccacacgca gcacgttgga gatcagccct tctgctgcct gtcgctccgt
     2221 aatggttttg ccgggaatga cgcccgacgt attaccaatg ccgtcggtcc agacacccgc
     2281 gctgcactga tacggctgca gacgacagcc ttcgtaatcg gcaatcagtt tcagcccctc
     2341 cacggaggtg tgaagctgct gaaaacccgg cagcgtggca gcaatagcca gcacggcccc
     2401 gacaaggcag cgtttaacga ttgatggatt catagtcctc ccgcgagatc tgcccgtcgc
     2461 gcagaagctg gtaggctttg tgtttgtagt accagttgat agccagcatc agcacaccaa
     2521 tcatcaggcc gcccagcgtt gaggcatcct tgatggacaa atcgcccagc caggccagca
     2581 cgacggcgat gcaatacgtg ataaaggcgc tgattcgctc aagcgtcata attcagtccc
     2641 atagctggac ggtctgcacg gtggtggttg tcggtatgtc cggcagatcc acctgcagcc
     2701 cgtgaggtaa aaaggggccg tattcggcaa gccccggatt tgccttcagt acctgctccg
     2761 tgacaccctg cgtgcgcccg taatgacgcc agcaaagcgc gtccaccgtg tcatactgat
     2821 gcgcacgcac tttcatcaga taagctccac tgtgcagtgc ggcgcatcct gcacccggct
     2881 gatggcccag cgggcatcac gccacaaatc accacttgct tccgccagtt cctcgcctcg
     2941 cttcacaccg gacgccgtgg cgtcatagtc ctgataacgc tcgttgagca tggcgcgtgc
     3001 ccagcagtaa accgcgttga aatagtgctg aatgcgctca cttttgccgt ccagctgttc
     3061 tgccggaacc tcagccagcg aggcatagcc cagcatctgc tggcgtctgc gaaactcata
     3121 cagctcggcg ttgacctccg aaattgccga cagcgcaacc tgctttaaac gcggctgcgt
     3181 caccgtgccg tcagtgcgca tcacactgcg aaactccgac aggtccacat caggccagaa
     3241 cggcgtattt ctgatgattt ccgcctgttc cggtgcctgt tctggcgcaa caaacttcat
     3301 gctgctttct cctgaaataa agggcggtgg acggggtttt gatgtggcag agcctttcgc
     3361 caccccgtgc cgcccgtgcg cgggggcacg ttctgtcagc ggctgtcatt gcgcagtctg
     3421 cgctccagct gctgtttgtc ttttttcacg ccacagcggg gatcgagctg taacgcatgg
     3481 ttgagatgat taatggcgga cgccggattg ctttcactca ggacagcacc aatcgcttta
     3541 tgcagacgcg cacgtgactg gtccggcata tccagaccgt ctgtcagctc cagcgtctgc
     3601 agcaacagat cggcatcaaa cccggtggcg gcaagcattg cactctgggc tgcgtctgcc
     3661 atttcctctg ccagcacggt ctgcacgttg cggttaccca gcggcatcac ccagccatga
     3721 cgcagggcat gacgcccgat ctccagcgcc ccggcataat ctccggcatc aatgcgccac
     3781 agcatcacgt acatcagcac gtcatcctgt tgagcgcctc cggcagccag gacgccctcc
     3841 gcccaggcgg catatttcgg cagcagctcc accttgattt ccgctttttt gaccgtggac
     3901 tgaacgccct tgagacggcg gcggtcttcc gccagttgca gcagcatcag gtcatagccc
     3961 aacgcgtggc gaacactgcc gccctcgcgg gcggcctgtt cagcctgaac gcgcaggcga
     4021 tgctgccgtg cgggactcag gctcatggat tacgctccgg tttctgctgc gacggcgctg
     4081 aaatcgccaa tctggatgtt ttccaccagt gcggcgcagc ggtagtcctc aaccacatag
     4141 gcttcattaa cggattcaaa gttttcaatc cggtcacgtt tcgggttgtc gataactgaa
     4201 cggcggcggg tgtcttcctg ccagtagatg gacaggttat ccagacgggt gatcagcagc
     4261 gcattcggcg ggaagaacgg cgcacgcacg gcctgcaggc cacccatgcg tttctgactg
     4321 atgatcatat cggcagccag tttttcactg ttttcctgct ctttgttgac cagcgggaaa
     4381 tacttgtcag acagcagttc acgaccgcaa atcaccacca gatcgtcatc atcctggtag
     4441 accacgtcga taagctcatt gaccgcatcc atcactacag cgtccaggtt ggcatattcg
     4501 ccacctttcc cgactttcac cgcacccggt gtggtttcgc cgcccgtggt ggtgctgccc
     4561 atgacgtgat ccggtgcatc ctcacggatt ttctgcagcc agcctttgtt cacatcctgc
     4621 agcagcgggt tttcactacg gttggaggtt ttcgcacgct tcacgccgtt aaagccgatc
     4681 atgatgcggt ccagtgcctg acgtttcacg atggcgtcac ggatacgcac ctggaaatcc
     4741 tgaaacttcg cccacaggtc cagcttcgcg taggtcagca ccgtgtcaaa gttggtctgc
     4801 tcgcatttat attccacatc gaccatcagc gtcggatcga caggttcacg ctctttcgcg
     4861 gtggtgtcag tggttccggc aatggtgctg ccaacaccca gccccagcag ctgaccggac
     4921 tgctcagtca ctggcgtgac gttaatcagc gtcaggaaag cggcggactg ctggatctgg
     4981 tcttccagcg tctgctgcac agacggctcc acagtgaatt tgctggacag ttcttcaact
     5041 gccacaccgt tcagacgcgc cagctgctgc aggtaagcgt taaaagcaaa gcgggtattc
     5101 ttcttcatcg ggttttgtgc tccatcagca attggtcaga gtgtcagcgg gggcgttacc
     5161 gcctgttgca cgctggcggt agtcctggcg gctgtcttca tgactcagct tgtccaccag
     5221 ttcgttaaag gcggtttgct gtgcctgcag ggcagtctcc agctcagaca ggcgttcttc
     5281 ctgctcagac agggattttt cggtgcgtgc gctcaggttc tgctgctcag tggcgaccag
     5341 ctccacggcc ttatgcacat cagagaaccg ggcgtcatcg gactgctctt ttttggtaaa
     5401 cagcgccgtg acgcgggcaa acagggacgg tttgtcgtcc tggacttctt ccagttcgat
     5461 caccgtttcc tctgcagcgg taaaaagatt ggcgggattc tgcttgcggt ttgccagcgg
     5521 gttatgggct gcactggcgc tgaatgtcag catttccgta cccagactgg cgggatcatc
     5581 agtggcagcc aggccgacca gataggcttt gcccgtatca gcaaacttcg ggctgacttc
     5641 catagaggtg aataatttct ggcctttttt caccagttcc accagggact ccgttggctc
     5701 aacgtcggca tacaatgcca tcttgcctgc cagcggacct tccgtgattt cttcagcaaa
     5761 cagcgccgtc accttgccgt agcggttaaa ggtgctgtcc ggcagataag acttgatgtg
     5821 ctcaaggtta atcagcgcgg tgtacaccgc cgggttgtag ctggctgcca tctgttccag
     5881 ccattcacgc tggatttcgc gtccgtcggt ggtggcacct tccaccccga tgcgaaaacg
     5941 ctttgctttc actgtcatga gccgtgctcc gttagaaaaa acttactgga gccttatggt
     6001 tgcggtgatg ggggcagtga aacaatgcgc ggtatttgta ccgacaacca cacaaaccgc
     6061 aggcggggaa agccttcatt caaggctgta ggtttgtgcc atgaacacca cactgacacc
     6121 cgcagatctc gatccccgtc ggcaggccat gctgctgtac tttcagggat accgcgtcgc
     6181 ccgcattgct gaaatgctgg gcgagaaagt tgcaaccgtt cacagctgga aaaaacgcga
     6241 caagtggggt gactatgggc cgctggatca gatgcagctc accaccgccg cacgctactg
     6301 ccagctcatt atgaaggagc acaaagaagg gaaagatttc aaagaaattg acctgctggc
     6361 acgccagtct gagcgccacg cgcggatcgg caagtttaac aatggcggca acgaagccga
     6421 cttaaaccct aacgttgcca accgcaacaa aggcccacgt cgtcagccgg aaaagaatgt
     6481 tttcaccgat gaacagattg agaaactgga agaaatcttc cattcctcta tgttcaacta
     6541 ccagcgccac tggtgggaag ccggaaaaac caaccgcatc cgcaacctgc tgaagtcacg
     6601 ccagatcggc gcgacctttt actttgcccg tgaagccctg attgacgccc tgctgaccgg
     6661 gcgtaaccag attttccttt ctgccagcaa ggctcaggcc cacgtcttta aacagtacat
     6721 catcgacttt gccaaagaag tggaggtgga gctgaaaggc gatccgatgg tgcttcctaa
     6781 cggggccacg ctttacttcc tcggcaccaa tgcccgcacg gcccagagtt accacggcaa
     6841 cctgtatctg gatgaatatt tctggatacc gaaattccag gagctgcgca aagtggcttc
     6901 cggtatggct attcacaaaa aatggcgaca aacctatttt tccacgccat ccagtctgac
     6961 acacagtgct tatccgttct ggtccggtgc gctgttcaac cgtgggcgca acaaagccga
     7021 taaggtggac atcgacctgt cccacagcaa tctggccccc ggcctgctgt gcgcagacgg
     7081 gcaataccgc cagatagtca ccgtagaaga tgcggtgcgc ggcggctgta acctgttcga
     7141 cctcgaccag ctacgcatgg agtacagccc ggacgaatac cagaacctgc tgatgtgcga
     7201 gtttgtggac gatctcgcgt ccgtgtttcc gctcagcgag ctgcaggcgt gcatggtgga
     7261 cagttgggaa gtctggaccg actttcatgc actggcgctg cgcccgtttg gctggcgcga
     7321 agtgtggatc ggttatgacc cggcaaaagg tacgcaaaac ggcgacagcg ctggatgcgt
     7381 ggtggtggca ccgccagccg tgccgggtgg taagttccgc attcttgagc gtcaccagtg
     7441 gcgcgggatg gacttccgcg cccaagctga cgccatcaaa aaacttaccg aacagtacaa
     7501 cgtgacttat atcggtatcg actcaaccgg cgttggtcac ggggtttacg agaacgtgaa
     7561 agcgttcttt cctgccgtcc gggagtttgt ctacaacccc aacgttaaaa acgccctggt
     7621 actcaaggcc tacgacatta tcagccaccg ccgtctggag tttgacgccg ggcacaccga
     7681 cattgcgcag tcattcatgg caatccgtcg cgcaaccacc gccagtggca accgcccaac
     7741 ctatgaagcc agccgcagcg aagaagccag ccacgccgat ctggcctggg caacaatgca
     7801 cgcactgttt aacgaaccac tgcagggcga gtccgccaat accagtaata ttgtggagat
     7861 tttttgatgg gaaagagtaa gaagaaccgc gctgcgtcga cgaaccagat ccagcataaa
     7921 agtcagacta cagccgaagc attcagcttc ggtgatcccg ttcctgttct ggaccgccgc
     7981 gaactactgg actatgtgga atgcgtacag acagatcgct ggtatgagcc gccagtaagc
     8041 tttgacggac tggcgcgcac cttccgcgcc gccgtgcatc acagttcacc aattgcggtg
     8101 aaatgcaaca ttctgaccag tacctacatc ccccacccgc tgctcagcca gcaggctttt
     8161 tcacgttttg tgcaggacta tctggtattt ggtaacgcct acctggagaa acgcacgaac
     8221 cgcttcggtg aagttatcgc ccttgagcct gctctggcaa aatacacccg acgcgggtta
     8281 gacctggata cctactggtt tgtgcaatac ggtatgacaa cccagccgta tcagttcacg
     8341 aaaggcagca tttttcatct gatggaaccg gatatcaacc aggagatcta cggcctgccc
     8401 ggttatcttt ctgccattcc gtcagccctg ctcaacgagt ccgccacgct gttccgccgc
     8461 aagtattaca ttaacggcag tcatgcaggc ttcatcatgt acatgaccga tgccgcgcag
     8521 aaccaggagg acgtgaacaa cctccgcaac gcaatgaaaa gcgccaaagg cccgggcaac
     8581 ttccgcaacc tgtttatgta ctcgcctaac ggcaaaaaag acgggcttca gatcatcccg
     8641 ttgtcagaag tcgcggcgaa agatgaattt ctgaacatca agaacgtcag ccgcgatgac
     8701 atgatggcgg cgcatcgtgt tccgccacag atgatgggga ttatgccgaa taatgtcggg
     8761 gggtttgggg atgtggagaa ggccagccgc gtttttgttc gaaatgaact gatccctcta
     8821 cagaagcgtt ttgaggaact gaatagctgg gccaatgaac aagtaattcg ttt
//
LOCUS       DADSZD010000168         8804 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.169, whole genome shotgun sequence.
ACCESSION   DADSZD010000168 DADSZD010000000
VERSION     DADSZD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.169"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            218..1312
                     /locus_tag="J1Z28_003705"
     CDS             218..1312
                     /locus_tag="J1Z28_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase A"
                     /protein_id="HBA7718503.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1336..1548)
                     /locus_tag="J1Z28_003706"
     CDS             complement(1336..1548)
                     /locus_tag="J1Z28_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBA7718504.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1808..2116
                     /locus_tag="J1Z28_003707"
     CDS             1808..2116
                     /locus_tag="J1Z28_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2755 family protein"
                     /protein_id="HBA7718505.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2175..3269)
                     /gene="yaiW"
                     /locus_tag="J1Z28_003708"
     CDS             complement(2175..3269)
                     /gene="yaiW"
                     /locus_tag="J1Z28_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBA7718506.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSSPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3282..4502)
                     /gene="sbmA"
                     /locus_tag="J1Z28_003709"
     CDS             complement(3282..4502)
                     /gene="sbmA"
                     /locus_tag="J1Z28_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBA7718507.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFALFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4854..6011
                     /gene="ampH"
                     /locus_tag="J1Z28_003710"
     CDS             4854..6011
                     /gene="ampH"
                     /locus_tag="J1Z28_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBA7718508.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6012..6635)
                     /gene="iprA"
                     /locus_tag="J1Z28_003711"
     CDS             complement(6012..6635)
                     /gene="iprA"
                     /locus_tag="J1Z28_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBA7718509.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGSCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6721..8742)
                     /locus_tag="J1Z28_003712"
                     /pseudo
     CDS             complement(6721..8742)
                     /locus_tag="J1Z28_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         2246 a         2243 c         2097 g         2218 t
ORIGIN      
        1 aaaaactcac cacatggtca tttaagccag gaataaatta gcttcccgtt catcattttt
       61 gtgatcttgc acacatcttt gagataacgc actttcttca ccagtacgtt gctcgctttg
      121 tcagcagaag attgcaacat tctgtcagaa ggccgcgaat cccccgtttt gtcgtggtaa
      181 tgtatgcact ttgtgttaga cgggataggt ttttaagatg gaaaaactgc gggtaggaat
      241 cgtttttggt ggtaaatcag cggaacatga agtgtctctg caatcggcaa aaaacattgt
      301 cgatgccatt gataaaagtc gcttcgatgt tgtgctgctg ggcattgata aacaagggca
      361 atggcacgtc agcgatgcca gcaattatct gctaaatgca gacgatcctg cccatattgc
      421 gttgcgccct tcggcgacca gccttgcgca ggtgccaggt aaacatgagc atcagcttat
      481 cgacgcacaa aacggtcagc cgttgccgac ggtggatgtc attttcccta ttgtccacgg
      541 tacgctgggc gaagatggtt ccttgcaggg aatgctgcgg gtcgccaatt taccgtttgt
      601 aggttctgat gttctggctt cagcggcctg tatggataaa gatgtcacca aacgtctgct
      661 gcgcgatgcc gggctgaaca ttgcgccatt tattaccctg acgcgcgcta atcgtcacaa
      721 catcagtttt gccgaagtgg agtctaaact ggggttaccg ctgtttgtaa aaccggctaa
      781 tcagggctct tctgttggtg tcagcaaagt aaccagtgaa gaacagtaca caattgccgt
      841 cgatctggcg ttcgagttcg atcataaagt gatcgttgag caagggatca aaggtcgtga
      901 aatcgaatgc gcagttcttg gcaacgataa cccgcaagca agcacctgtg gcgagatcgt
      961 actcaccagc gatttctatg cctacgacac caagtacatt gacgaagatg gcgcgaaagt
     1021 ggtagttccg gcagccattg cgccagaaat caacgataag atccgggcga ttgccgttca
     1081 ggcttatcag acgttgggat gcgcaggcat ggcgcgtgta gacgtgtttt taaccccaga
     1141 gaacgaagtg gtgatcaacg agatcaacac cctgcctggc ttcaccaaca tcagtatgta
     1201 tccgaagctg tggcaagcca gcggtctggg ttacaccgac ctgatcacgc gtttgattga
     1261 gctggcgctg gagcgtcacg ctgcggataa cgcactgaaa accacaatgt aatattcaaa
     1321 cgcccggtaa tattcttatt gggcgttttc agtttcttca tcgcgacgac gaataatcaa
     1381 ccccgccagc cagaaactaa tcacccatgt caccagaccg acggcatagg tctgccaacc
     1441 ttttgcttca aatcccagta accccaccac gccattaaga atgaatataa ggccaatggc
     1501 gaacgcatag tagtgccagt cacggcggat ttttacaggc agattcatgg cagctccgtt
     1561 aacgcaaaac aacatccttg catattctgt aacaactgtc tgtggtgtag acaacacttt
     1621 cgcaattgtt aataaattta acttaaggtt gctattttgt gactcacaac gaagattcac
     1681 gcttcgggaa tattcccaac atcaatttac catgattctg atattgacaa tctaaatgac
     1741 ctgtcagact tgccataagc cgctggtatc acaccagaag agaagcagat gacttccgga
     1801 ggtttttatg gctgatttca ccctgtcaaa atcgctgttt agcggaaaat atcgcaatgc
     1861 ctcttcaacg cctggcaata ttgcctatgc gttgtttgtg ctgttttgct tttgggctgg
     1921 ggcacaattg ctgaacctgc tagtgcatgc gcccggcgtc tatgagcgtt taatgcaggt
     1981 ccaggaaaca ggtcgcccac gggtggaaat tggtttaggt gtcggcacca tttttgggct
     2041 gatcccgttt ttagtaggct gcctcatttt tgcagtagtg gcgctatggc tgcactggcg
     2101 acatcgccgc cagtaagatt tacgttgcca gatgcggcct aaaacgcctt atctggcgtg
     2161 acattcctgt ttgatcactg tttcatacaa cgcgcccgcc gttcgtctac gcgcttcgca
     2221 aaccaggccg tagtcagatt gcgggtgatt ttcgggcttt ccagttgaat gccaggcaac
     2281 atttcgcggg gtagtgattt accggttttc gcttcggcaa gttgataaac tttcttgtac
     2341 agcgccgtct cctcaaaaga gaagctgtcc cctttctcta actgacggcg aatttcgctg
     2401 tcgctcatcc ccagttttcc cgccagtttg cgcgttgcca gttcggtttt cccgggctct
     2461 ttactgtcgt aacgaattaa atcaccgtcc agtgccagct tcacgccgct ggccttactg
     2521 accgcgttct gaaacgcggc attacggctg gcgtaccagc cagcgttaaa atcagcaaaa
     2581 cggtatatcg gcgagctata gctggcggga taattcagta aatggtaagt accaaaccac
     2641 aacccgccgc ggcgactgaa gacttcctga cggactgtac cgtccatttt ccacggatac
     2701 cctttggtat gctgttcggc aaaagcaatg ctgacctgca tcggcccacc tgtgcgcacc
     2761 ggattgagcg aaccaaacag cgtctgcccc attggcacca tgctgatcag atcgtcaaaa
     2821 atcgcactca attgcttttc tgtacgcacc gaatctaacc gttcgctata gcttttgccg
     2881 tttggcgatt tgatttttag cgcggtatgc accagaaaag caggaatgtg catccgttca
     2941 gcacgacggt caatctcttg ccaggcgatc ttgcttaagc ccggaacggc cggatctgcc
     3001 tgataatttg actcctgctg cgccaccgcc agcaccgagc agacgttttc cagcgtcggt
     3061 gccagcccct gactttcaaa ggtggtggcg agatctttcg cccaggcatc gcggtctttc
     3121 acgctcgcag gcatcttctg ccgcacgacg ctcgccacat ctatcgcttt ttcgcccttt
     3181 ttcaacggtt gcggagcctg actactacac cccgctaaaa ccagaaccgc caacaatgac
     3241 agcgaagata gaggattgac gcgcgacatt acttctcctt tttagctcaa ggtatgggtt
     3301 acttcctgaa ttttgtcacc atccagctca tgttcaaagc tgcgcagacg tttgtagata
     3361 gacatcaact caaccagtgt ggtccatgag ttaatcaggt actggaaagc accgcgaacc
     3421 tgaccaaaaa cgttagtaat ctgcgtcatc aggccgagtg taatcgtacc ggcaacaatt
     3481 gacggaaaca gcaagaacaa accgaaaacg ttatcgacct gcaaatagag aatgcgggcg
     3541 atattgaaat acatatagtg aaaatagagg cggaaatagt ttttccgtac ggcgctaaaa
     3601 agctcgcgta ccgtaggcgg cgtcgcgcgc gtggcatcgt cttcaccata aaccagctct
     3661 ttacggtagg cagcctctac acgctggttt ttaaactcca gccccggcag tttgatccct
     3721 accactgcca gtaatccggt ccccatcagc gaccagacga tagcggcaat caccagacca
     3781 tacggaatgt gcccgacaat cggcagctcc ggcacatgtg cggagagcgt taccagcacc
     3841 ggcaggaagg cgatcaacgt catgatagcg ttgataaagc tgacgcccat attctccagc
     3901 gttgaagcaa aacgcatggt gtcttcctgc acacgctgcg cggccccttc gatatgacgc
     3961 agttgttgcc agttcgccat gtaatattcg ttcatcgccg tacgccagcg gaacacgtag
     4021 tgactgacaa agaagttgtt cagcacactg atcaccaccg cgatcagcgc aatccccaga
     4081 aagacgccca cttcgcggta aaattgttcg atggtgactt tatgcggcga acttagcgcc
     4141 gtttgaatca gatcatagaa cggcgcatac caggcgttga cggcaacccc gacttccacc
     4201 aaaaaccagg tgacaaagat aatcagcgca gtaccgagta ttgaccagta ttgccaacgg
     4261 tgcgggctat agataaacca gaacaatgca aaaagtccta cgcaaacaat gtagtaagcg
     4321 taaaaaatca ggaaatccaa cgaccagaaa cgcgcggcgc taatcgggat ctgcccggaa
     4381 gcgccggtga tacgcgccac ccaatcaccc ccaccggctt gccagaagat aacggcaatc
     4441 aatgcccaaa caaaggccga gagaaaaaac gttcccggct ttgggaaaaa agacttaaac
     4501 atatgcactc ctgctaactt cttatcgttt taacaactgc tgtgtattaa ccgcatgacc
     4561 gctacaaagc gtcagccaaa tgaaaccgcc cattgtgacc catagcaagg cgcttagttc
     4621 gccagcctga ccataagaat tgtttcgacg ggtttcacaa accaaagatt gtttacataa
     4681 gcggattaac acgcgcagga tcacaaatgc aggcattggt gagcaggaaa aagatcgtgt
     4741 ttacgcgccg ttacgttttt aacactatta ttgcactaat tcgccctttg caatccatca
     4801 atgagtagta taaatacgct cagttacctt cattcaatct atggacacca ccgttgaaac
     4861 gtagtctgct tttttctgcc gtgctgtatg cggcgtcatt gacctccgtc catgcggcgc
     4921 agccgatcac tgaaccggag tttgcctctg atattgtcga tcgttatgcc gatcatattt
     4981 tttacggcag cggtgccacg gggatggcgc tggttgttat cgacggtaac cagcgcgtct
     5041 ttcgcagtta tggcgaaacg cgacctggta ataacgttcg cccgcagctg gattccgtca
     5101 tacgtattgc ttccctcacc aagttaatga ccagtgagat gctggtgaaa ttgctcgacc
     5161 aggggaccgt gaagttaaac gatccgctaa gtaaatatgc tccgccaggc gcacgtgtac
     5221 cgacctacaa tgggacgccc attacgctgg tgaatctggc aacccatacc agcgccctgc
     5281 cccgtgaaca gcccggtggc gcggcacatc gtccggtatt tgtctggcca acgcgcgagc
     5341 aacgctggaa atacctttcc actgccaaac taaaagccgc gccgggcagc caggccgcgt
     5401 actccaacct cgcattcgat ctgctggcag atgcactggc gaatgcgtcc ggcaagcctt
     5461 atacccagtt gtttgaagag cagattaccc gtccgttggg gatgaaagac accacctaca
     5521 ccccctcacc ggatcagtgc cgccgtttaa tggttgctga gcgcggtgcc agtccgtgca
     5581 ataacacgct ggcggcaatt ggcagcggcg gcgtctattc cacgccaggc gatatgatgc
     5641 gctggatgca gcagtatttg tcatctgatt tttatcagcg cagtaaccag gctgatcgca
     5701 tgcagacgtt gatttatcag cgcgcgcagt ttacaaaagt gattggcatg gatgtccccg
     5761 gcaaagcgga tgcgcttggt ttaggctggg tatatatggc cccgaaagag ggtcgtccgg
     5821 ggattattca gaagacaggt ggtggtggcg gtttcattac ctatatggcg atgatcccgc
     5881 agaagaatat tggtgcgttt gtggtggtca ctcgttcgcc gttgacgcgc tttaaaaata
     5941 tgagcgatgg catcaatgac ctggtgaccg agctaagcgg gaataaaccg ttggttatcc
     6001 ccgcatcctg attaatactc tgaaggcaaa cggttgatag cgaccagttt gcctttattc
     6061 atttcgatat agccgccttt acgcaaagca gcgagaactt ccgcaacgac agaacgcgat
     6121 atacgtgtac gttgatggat ataattcatc acgccaatac gtgatcgcaa ttcttcattc
     6181 cagtcaatca ttgataataa cgtcgcgcgg atttgttcgt aggaattatg cccaatgagc
     6241 tgaacgtcgc gtaattccag aattctattt tgccaggcta accagtaaaa agcgtctcgc
     6301 cagagttgat tttgttcaat aagagtaatg gtttgtttgg ctggcaaatg atatcccgta
     6361 cagcttcctt ctgatattaa tttgtatggt atatcgtttt tcattaaacc atcagccagc
     6421 cccataatat aaggcgcctg cgtaataccg ataagtacgt tttcttctct acgcagagag
     6481 ataacgccct ctaaaataac gaaagtatct tcgctattaa catcactgga aaatataact
     6541 tgcttttcat tattaaactc gaagcgcgta ccgtatctgg acaaacattt atcgagctta
     6601 ccaaattcct gaagaggttt aactacagat aacatttgcg cgtcctttgc agtaatgccc
     6661 gtcaaatcct tgacgggcat tatttagatt aaattaccta tttcttcgga gtgaagaata
     6721 ttaccaggta tatttaacac ccacattcgc ggaccagtct tgatctacgt caccaccacc
     6781 gaggtagtta gcatcggtat aggcgctgaa gttcttggtg aagctgaact gagtacccag
     6841 accaacacgt accgcagacc cttcagtacc gttatcgatg gaatcaccgt tcacatcgtt
     6901 atcgttgtta gagtcgtcgt agacgtaagc cagtttgaag tacggagtca gagcctggtc
     6961 ttcgctgtag gtgaaggtat aacctgcatc tacacccagt tcataacgca tgctgtcgta
     7021 agactgacca tcaactttca tgtcgttgct cagctggtaa tcatcaccag actggaacag
     7081 accagaaacg ctgccgtaag gagtcacgta acctgcatca cccagtttga agtcgtaacc
     7141 ggctttcaaa ccaaagcccc aagcgtcgga gttggtgctg ccgtcaacgt aagtaccgtt
     7201 gctcatggtt gcagacaggt cgttgttgaa gtgagagtag ctcaagctac cgtcaacaaa
     7261 gacgttgttc gcgaagtgag cagaagagta gatgtaggca gtctggctgt cttgatccac
     7321 ctgaccagaa cggtcattca tatcaccttt agcgaagcct gcagccgcac cgacgatcca
     7381 cttagcgttg ttaccgtcaa ttttggtatc aacaccgacc atgatgccgt taacatcctg
     7441 atcatagttg atggtgccgt tgtcgccgtt gaagttacca ccgaagtagc ttacccatgc
     7501 gccgccgtta tcagccagac catgacgaga gttggtcaga cgagtaccaa cggtgtcttg
     7561 ttccaggttc cagatattgg tgttcgcaga cgggatgctc agcgccatgt tagcgtagtc
     7621 ggtcaactcg gtctgatgca gaacaacggt gttaccacgc tgctcagcct gataggtgta
     7681 tgcacccagg tcagctttgt tagcagcaga gaaggtcgcg ttggtgttga cgtcgtttac
     7741 atagataatt tctttatctt tgtaatcagc gattgcgcca gcgccagttg cgttgtcgat
     7801 acgaactttg tagttaccgg ttgccgcaac aacgttgtct acagagctgt tatccagttc
     7861 agtaccgttg tctacgtcgc cgttaccgtt gatagtcaga tgaccatcag agttcatagc
     7921 aacaacaccg tagccgtagt tagcctgagc ggtatcccaa gtacggtcgt tcaccaggtc
     7981 agcgttcaga acataatcgc tgctgttgat atcgaacaca ccagaaacta cattgccgtt
     8041 gtggtcagcg atgttgccgc tagtcagttc cagtttgtca gtacggaaag cttcagtatc
     8101 aaactgatca acgttcacat ccagtacacc accgttagtt acggtgatgg tattggcata
     8161 caggttagaa tagttattcc agccagtaga ttcggttaaa ttaacggtac tgtagctatc
     8221 aacagtcagg tggtctgtag caaccaaacc atcttcacca atattcagtg aggagccgtt
     8281 ggttaaacca atggtatctg agaccagaga ggaatcagta acatttacct gagagccatt
     8341 atttacagct aatgtatcaa ttactgaact attaacggta ttccattctg aaccattgtt
     8401 cagtgctacg ttaaatacgc cgctctggaa taccttgttg tcaataatac gaccccaatc
     8461 gttagcttcg atagtggcac caggggtcag gctgttcggc gcaacatcat acatatctgc
     8521 cggagcaatt acttcagcac tcatgtttgc tgagccaacc cacttactac cgttgttcag
     8581 agtaatgttc agttcatcaa cgttctgagc atcatctacc caaccaccgt tggtatcaag
     8641 gacaccgtca ccattgctgt catggccggt tgaatagaag aatacgccat cgttattcca
     8701 gtggcttgtg aactcgacat cacccaacag ggttgaatgg tcaaagttta cattgttttt
     8761 catggaataa tttgagccag cattgtcaga gaatgacaga gcta
//
LOCUS       DADSZD010000169         8560 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.170, whole genome shotgun sequence.
ACCESSION   DADSZD010000169 DADSZD010000000
VERSION     DADSZD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.170"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            347..1156
                     /gene="pduF"
                     /locus_tag="J1Z28_003713"
     CDS             347..1156
                     /gene="pduF"
                     /locus_tag="J1Z28_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBA7718510.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCVAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNIVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWFAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKEISE"
     gene            1359..2273
                     /gene="pocR"
                     /locus_tag="J1Z28_003714"
     CDS             1359..2273
                     /gene="pocR"
                     /locus_tag="J1Z28_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HBA7718511.1"
                     /translation="MISAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFT
                     PFCQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGF
                     VLCGQVRLRNDDVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVE
                     NCLKKQLNFVVIKENKSQSDSVRQTRAPGPHDNKMKKALRYIDAHLSDDLRLEDVASH
                     VYLIPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYF
                     CKVFRQTYQVTPQVYRQQVSDNAAMETV"
     regulatory      2365..2540
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2758..3195
                     /locus_tag="J1Z28_003715"
     CDS             2758..3195
                     /locus_tag="J1Z28_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBA7718512.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PFDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLNGPA
                     GGAVALIHYLNEICHARETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            3329..3874
                     /gene="cobU"
                     /locus_tag="J1Z28_003716"
     CDS             3329..3874
                     /gene="cobU"
                     /locus_tag="J1Z28_003716"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBA7718513.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WNYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRINQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            3871..4614
                     /gene="cobS"
                     /locus_tag="J1Z28_003717"
     CDS             3871..4614
                     /gene="cobS"
                     /locus_tag="J1Z28_003717"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBA7718514.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            4626..5705
                     /gene="cobT"
                     /locus_tag="J1Z28_003718"
     CDS             4626..5705
                     /gene="cobT"
                     /locus_tag="J1Z28_003718"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBA7718515.1"
                     /translation="MQILADLLNTIPAIDPPAMSRAQRHIDGLLKPVGSLGKLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGWDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVILGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMGMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            5767..6693
                     /gene="ldtA"
                     /locus_tag="J1Z28_003719"
     CDS             5767..6693
                     /gene="ldtA"
                     /locus_tag="J1Z28_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBA7718516.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQVIDQPVKYTMEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVNINRNGF"
     gene            6867..7832
                     /locus_tag="J1Z28_003720"
     CDS             6867..7832
                     /locus_tag="J1Z28_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718517.1"
                     /translation="MTKQNKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIANNLRKKFQLEGAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSLHEGMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGDPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLCKIFPDTWCEALFQQDIAADVCISLVSREGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(7884..8342)
                     /locus_tag="J1Z28_003721"
     CDS             complement(7884..8342)
                     /locus_tag="J1Z28_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBA7718518.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDVTFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAAS"
     gene            complement(8353..>8560)
                     /locus_tag="J1Z28_003722"
     CDS             complement(8353..>8560)
                     /locus_tag="J1Z28_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBA7718519.1"
                     /translation="KEEKIEAQVDKSSLMACTSDTFNSYDLVVSSTKLDNPAITTRVI
                     VGAGLLTGLGEDEIFAAVKEEMLK"
BASE COUNT         2131 a         1780 c         2230 g         2419 t
ORIGIN      
        1 caatgtgaat taattgcgtt gtctagtgag tttaatcgcg agtaatattt cgttgagttt
       61 ggttcgagga aggaactgtg atatttgttt caattttatt atgtcatgat ggcaattttt
      121 tactgtaaaa gtttggtggg aattattact ttcaacactg attaacgctg tgttgcatga
      181 ttcggcaatg acaataattt gttatgttag cacgattttt acacgagtct gtcatgcatg
      241 ttggcaatat tttctgcaga gcaatttttt atagttgcgc tcggtataca caacaatcag
      301 cgcatagttg gctgataaag aaaaactccg taaagaaggt gtcactatga atgattcact
      361 caaggcgcaa tgcgttgccg agtttttggg caccggactg ttcctctttt ttggcattgg
      421 ttgtttgtgc gccctgaaag ttgccggggc aagtctggga ctgtgggaaa tttgtatcat
      481 ttgggggtta gggatttcgc ttgctgttta ccttacggca ggtatttccg gcgctcatct
      541 caacccggca gttaccattg ctctgtggct gtttgcctct ttcccagccc gtaaagtgct
      601 gccattttgt gtggcgcaat tagcgggtgc ctttggtggt gcggttctgg catattctct
      661 ctacagcagt ttgtttaccg attttgaatc tgctcacaat atagtgcgtg gtagcgcaga
      721 aagtttacaa ctcgccagta tcttcagtac ttatccagca gcggcgatta atgtatggca
      781 agctgcgttg gtcgaagtgg tcataacgtc catgttaatg gggttgatta tggcgttgac
      841 cgatgacggc aatggcgtac ccaaaggacc gcttgcgcct ttactcattg gtattctggt
      901 tgctgttatt ggtgcttcta ccggaccatt aaccggtttt gccatgaatc cagcgcgtga
      961 ttttggacct aaaattttca cctggtttgc gggttggggg gaaattgcga tgactggtgg
     1021 acgcaatatt ccttatttca ttgtacccat tattgcaccg atcattgggg catgtgttgg
     1081 tgcggcgatt tatcgctatc tcattgcaaa gaatttacct gtcaatagtg taacgcctaa
     1141 agaaatcagc gaataacgaa ataatctggc agttataaac gtttattttt ttactgtttg
     1201 tgatctgctt tccgaactta ttaaaaagtc tcccgcttca cggaggcttt tgttattgtc
     1261 ctttgtgctt ctcgtcagta aatcatcaag gatgatgtga agattaaatg taaaacaatt
     1321 gttttgctat aacaataaat taactgggag gttttatcat gatttctgcg gcgacactta
     1381 attcagaact cattaataaa atcgcgcagg attttgcgca ggcgaccagc cttgcggttg
     1441 tcgtggtcaa tattcacggc gatgaaattt ccgagctgtt taacttcact ccattttgtc
     1501 agatgatgcg gcaacaccct cagctgagca ctcgatgtcg catgagcgat cgttgtgggg
     1561 gtctggaagc ctctaaatcc gatcagcctt gtatttatcg ctgtcatgcc ggattaaccg
     1621 atttctctat tccactggta atcgccgggc atttagttgg ttttgtattg tgcgggcaag
     1681 ttcgtctgcg taatgatgat gtcgatttgg tcgatattct gaatgtcgat gattgctggc
     1741 aggcagatcc cgaattactt aatgaatttc gcaaagtccc tgagatggat tactcaagag
     1801 ttatggcctc tgcggatttg ttgaagttga ttgtagaaaa ttgcctaaaa aaacaactca
     1861 attttgtggt tattaaagag aacaaatctc agtcagattc ggtgcgccag actcgtgcgc
     1921 ctggtcccca cgacaataaa atgaaaaaag ctttacgtta tattgatgca catttatcag
     1981 atgatttgcg tctggaagat gtcgcctcgc acgtttatct aatcccttat tatttcagta
     2041 agttatttaa gaaataccat ggaatcgggt ttaatgcgtg ggtaaatcaa caacggatgg
     2101 ccagcgctcg tgagttactg tgccatagtg actggagcat cgccagcatt gcgcgtaatt
     2161 taggtttttc gcaaacaagt tatttttgca aagttttccg tcagacttat caggttacgc
     2221 cgcaggtgta tcgacagcaa gtgagtgata atgcggcgat ggaaacggta tagagtaaat
     2281 gccccaaaaa aagaggacgg tttaaataaa ctctgcaatg aataattaac gttatttacc
     2341 tgttatggga aaagtgaaat cagctcataa catacgctga caggtgtggc acatttgtgc
     2401 ctgggaagag ggtgaaaatc cctcgcagcc cccgctgctg taatgctgac aaacccgaaa
     2461 atgccactga tcgcgagatt gggaaggtcg ggagaggatg acgctaagcc agaagaccag
     2521 ccagtcagtc aataccaaca acttcggtgg gaagtgggtt gcccggaagc gtccggtaga
     2581 gtacaggcgc gtctttatac ccctaccacc ctgaaaggat cagggtatgg cggcatcatt
     2641 tagttctttc atcgcagtgc cgacaggcac cagggctgca cttaaggtgt ggcaatcttg
     2701 tttcatggct tatcaaccag accgccatta caggaaatta agtggagggc gaggggcatg
     2761 aaaacgataa aacccgaatc gatagctctt ttttgcctct tatctggaga tgtggatctg
     2821 gcgaaacatc tggcggaaat attgccaata acctgttttg ttggtgagga ctggcagcaa
     2881 gatttccagc cttttgacgg tgggcctgtg caatcgttat cagatgcgta tagcgattat
     2941 tcagtgctgc tattgatagc agctgaaagc gagatatccg taccagaatg gctcgctcat
     3001 tctgagggaa cgacggtaat tttgattgca gaacacaatg cccgtttgct gaatggtccg
     3061 gctggtgggg ctgtagcatt aatccattat ctgaatgaga tctgtcatgc cagagaaacc
     3121 gctgtcaatg acgcattttg tcggacgtta acacgaggaa gtctggctat tccgcaatgc
     3181 catcttcttt cgtaatggat tgagaatgct ggatttttaa ttatattacg cgcagtatta
     3241 atcgcgtaaa tgttttgtgg tcagcaacgg tgggggagcc attgttctgt tcaatgaata
     3301 ccatcataca attgaatcag gagtcggtat gatgattttg gtgacggggg gcgcacggag
     3361 cgggaagagt cgccacgcag aggcgcttat tggggactct tcacaggttc tgtatatcgc
     3421 tacctcgcaa atccttgatg atgagatggc tgcacggata gaacatcatc ggcaaggccg
     3481 cccggagcac tggcgcacag tggagcgctg gcaacatctt gatgaattaa ttcatgcaga
     3541 cattaacccg aatgaggctg tgttgctgga atgcgttact acaatggtga ctaatctgtt
     3601 gtttgattat ggcggcgata aagaccctga tgaatggaat tatcaggcga tggaacaggc
     3661 gattaatgct gagattcagt cgttgattgc tgcctgccaa cgttgccccg caaaggttgt
     3721 attagtgact aacgaagtgg gaatggggat tgtgccggag agtcgtctgg cacgacattt
     3781 tcgtgatatt gccgggcgga taaatcagca gttggccgct gcggcaaatg aagtatggct
     3841 ggtggtttcg ggtattggag taaaaatcaa atgagtaaat tattttgggc gatgctctca
     3901 tttattacgc gtctgcctgt accgcgtcgc tggtcccagg gactggattt cgagcattat
     3961 tctcgcggta ttattacttt tcctttgatt ggattattgc ttggcgcgat tagcgggctg
     4021 gtcttcatgg tgctgcaggc atggtgtggc gtaccactgg cggcactgtt tagcgtactt
     4081 gtgctggcgc tgatgaccgg gggattccat ctggacggcc tcgctgatac ctgtgatggc
     4141 gtattttctg cacgtagccg cgatcgcatg ttggaaatca tgcgtgatag ccgtttaggc
     4201 acccacggcg gtctggcatt gatttttgtg gtactggcaa agattctggt gttaagcgag
     4261 ttggctctgc gtggcgaacc gatccttgca tcgctggcgg cagcatgtgc ggtcagccgc
     4321 gggactgccg cattattgat gtatcgtcat cgttatgcgc gggaagaagg gcttggcaat
     4381 gtgttcattg gcaaaattga tgggcgacaa acctgcgtca cactcggctt agccgctatt
     4441 tttgctgccg tattattgcc gggtatgcat ggtgtggctg ctatggtggt gacgatggtg
     4501 gcgattttca tcctcggtca gcttctcaaa cgtacgctgg gcgggcaaac gggcgatacg
     4561 ctgggagcag ccatagagct tggtgaactg gtcttcctgc tggcactgct ataaagagac
     4621 gtaacatgca aatacttgcc gatttactga atacgatccc agccatcgac cctcccgcta
     4681 tgtcgcgtgc acaacggcat attgacgggt tactcaaacc tgttggtagc ctgggaaagc
     4741 tggaggcgct tgccatacaa ctggcaggaa tgccggggtt gaatggcata ccgcatgtgg
     4801 gcaaaaaagc ggtactggtt atgtgtgccg atcacggcgt ctgggaggaa ggggtcgcta
     4861 tttctccaaa agaagtgaca gccatacagg ctgaaaatat gacccgtgga acaaccggcg
     4921 tgtgtgtgct ggcagcacaa gcgggcgcta acgtccacgt aattgatgtt ggtattgata
     4981 ctgctgagcc tatccccggg cttatcaaca tgcgtgtcgc acgaggtagt ggcaatattg
     5041 cttcagctcc ggcaatgagt cgccgtcagg ctgaaaagtt gcttttggac gtcatatgtt
     5101 atacgcggga gctggcaaaa aacggtgtca cgctgtttgg tgtaggtgaa ctggggatgg
     5161 caaacacgac accggcagcg gcaatagtca gcacaatcac tggctgggat cctgaagaag
     5221 tggttgggat tggcgcaaac ctgccgacag ataaactggc taataaaatt gatgttgtgc
     5281 gccgggcgat tacgttaaat caaccaaatc ctcaggatgg tgttgatgtc ctggcaaaag
     5341 tgggtggatt tgatttggtc ggaatagctg gagtgatatt aggtgctgct tcctgcggtt
     5401 tacccgtgtt gctggatgga tttctttctt atgctgctgc gctcgcagcc tgccagatgt
     5461 ctcctgcaat caaaccgtat ctcattcctt ctcacttgtc ggcagaaaaa ggcgcgcgta
     5521 tagcgctgtc gcatttgggg ctggagcctt atctcaatat gggtatgcgt ttaggtgagg
     5581 ggagtggtgc agctctggcg atgcccatca tcgaagctgc ttgtgcgata tacaacaaca
     5641 tgggcgaact tgctgccagt aatattgttc taccggggaa tacgacttct gatttgaaca
     5701 gctaaaaacg aaaagtatga taaaagctat acttatcgga tagtattcgc gacttaggaa
     5761 agggacatga tgcgtcgtgt aaatattctt tgctcatttg ctctgctttt tgccagccaa
     5821 aatagcctgg cggtaactta tccattacct ccagagggta gccgtttagt ggggcagtcg
     5881 cttactgtaa ctgttcctga tcacaatact cagccgctgg agacttttgc cgcacaatac
     5941 gggcaagggt taagtaatat gctggaagcg aatccgggcg ctgatgtttt tttgccgaag
     6001 tctggctcac aactcaccat tccgcagcaa ctgattttgc ccgccactgt ccgtaaaggg
     6061 attgtggtta acgtcgctga aatgcgtctt tattactatc cgccagacag taatacagtg
     6121 gaagtctttc ctattggtat cggccaggct gggcgagaaa ccccgcgtaa ctgggtgact
     6181 accgttgaac gtaaacaaga agcaccaacc tggacgccaa cgccgaacac tcggcgcgaa
     6241 tatgcgaaac gaggggagag tttgcccgca tttgttcctg cggggcccga taaccccatg
     6301 gggctgtacg cgatttatat tggcaggttg tatgccatcc acggtaccaa tgccaatttt
     6361 ggtattgggc tccgggtaag tcagggctgt attcgtctgc gcaatgacga tatcaaatat
     6421 ctgtttgata atgttcctgt aggcactcgt gtacaagtta ttgatcagcc agtgaaatac
     6481 acaatggaac cggatggttc aaagtggctg gaagttcatg agccgctatc gcgcaatcgt
     6541 gcagaatatg agtctgaccg aaaagtgcca ttgccggtaa ccccatcttt gcgggcgttt
     6601 atcaacgggc aagaagttga tgtaaatcgc gcaaatgctg cgttgcaacg tcgatcggga
     6661 atgcctgtga atatcaaccg taacggattt tgacattcaa aacgtctggt ttgtcggtct
     6721 gtgtaagcat tccgtcatcc agaacaggtt gtttcgaatc atacaaatga taattttctt
     6781 ctcatatgcg catctcttat gagcataaac gttatactct tctgatttat acatgtttgc
     6841 ggatgacatg atcaatggaa aagagaatga cgaaacagaa taaaaacagc cgttggcagg
     6901 gttacgatcc tcgttacata tactcgctcg tggtcagacg ctattttgcg gatatgaaaa
     6961 ccaagattga gattgctgag gagcttggcg tttcccgctt taaagttgcc agattgatcg
     7021 atgaggcgat cgaacaggat tatgtgaggt ttatcttccc taagcagcaa gcgatggatg
     7081 aagaaatcgc caataatcta cggaaaaaat ttcaactgga aggtgcaatt gttctctcag
     7141 ttgctgaatc ctggacgacg caagaagaac tcaatcacaa attgggtgaa gtcaccgctg
     7201 aatatcttat gcaatctctt catgaaggta tgaaagtggg gatcgcctgg gggcgtgtat
     7261 tatcgagcac ggtcagtaag ttgagcaagt tgcctccttt agacgttgtg cagttatctg
     7321 gtgtacatcc ggggatagag tttagtcaag ggccaataga tcttattcat aagatcgctg
     7381 ccatttcgca gggaaaagcg cacccaatgt acgtgccgat gtgggtcgat gacgaagagc
     7441 ttgctgccag actggcaggt gatccagcgg tattagatac acagcaatat tactcgcagt
     7501 tggatgtggt tatcaccggg ataggtgact ggaaatcagg ttcttcaagc ttgtgtaaaa
     7561 tatttccgga tacctggtgc gaagctttgt ttcaacaaga tatcgctgcg gatgtgtgta
     7621 tctcgttggt cagcagggaa gggaagattc ttcatagccc tattgaacgt ctgggatttg
     7681 gcatttcgac ggatcaacta caaaaagcca aaaaagtgat tggtgtcgct ggaggggagg
     7741 aaaaatatga aggcattctt gcttcgctga aatctggact tttaaacgtc ttaattactg
     7801 attttgatac ggctattaaa cttctggatt aataggcgta tcagtgaaga aagggcagta
     7861 ccgctgattc tgccctttta atttcaggat gctgcgagag tatcctctac atatttagct
     7921 acgcgcgttg caggtaaatt gcggcaagtc tcaacaaagg cgttatcctg aaacagtgtg
     7981 aataaacgac gcaatatcga catatgttga tcactggtgg caaatgccag gttaaacata
     8041 atgctacaat caactgttaa atcagcatct tccatttgcc cgaaagtgac atcattggcg
     8101 aaacgaacaa tactgatact gtttacattt accagcgtgt aatcggcatg gggaatggcg
     8161 ataccctgac cgtttgccat cattagccct gtcggatagc ttttttcacg ttccaggcag
     8221 gcgtcaatgt attctgcagt gatgtagcct ttttccaata attgcgtacc agcaaaggtg
     8281 atcgccgttt gatagtcact gatgctgtta tcaacatcat tcaccagaat ttcaaatttc
     8341 attgcacacc tgttatttca acatctcttc tttaactgca gcgaaaatct catcttcgcc
     8401 cagaccagtc agtaatccag cgccaacgat gacacgagta gtaatggccg ggttatcgag
     8461 tttggtcgat gaaaccacca ggtcatagct gttgaaggta tcgctggtgc atgccattaa
     8521 cgaagattta tcgacttgcg cttcaatttt ctcttctttc
//
LOCUS       DADSZD010000170         8235 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.171, whole genome shotgun sequence.
ACCESSION   DADSZD010000170 DADSZD010000000
VERSION     DADSZD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.171"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            108..1085
                     /gene="csiD"
                     /locus_tag="J1Z28_003723"
     CDS             108..1085
                     /gene="csiD"
                     /locus_tag="J1Z28_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBA7718520.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTNR
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGV
                     DDVKQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1105..2373
                     /gene="lhgO"
                     /locus_tag="J1Z28_003724"
     CDS             1105..2373
                     /gene="lhgO"
                     /locus_tag="J1Z28_003724"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBA7718521.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPAFHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2396..3844
                     /gene="gabD"
                     /locus_tag="J1Z28_003725"
     CDS             2396..3844
                     /gene="gabD"
                     /locus_tag="J1Z28_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBA7718522.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDNGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3858..5138
                     /gene="gabT"
                     /locus_tag="J1Z28_003726"
     CDS             3858..5138
                     /gene="gabT"
                     /locus_tag="J1Z28_003726"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBA7718523.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLSIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5376..6776
                     /gene="gabP"
                     /locus_tag="J1Z28_003727"
     CDS             5376..6776
                     /gene="gabP"
                     /locus_tag="J1Z28_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBA7718524.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            6797..7459
                     /gene="csiR"
                     /locus_tag="J1Z28_003728"
     CDS             6797..7459
                     /gene="csiR"
                     /locus_tag="J1Z28_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBA7718525.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     NVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSYYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7460..7909)
                     /gene="kbp"
                     /locus_tag="J1Z28_003729"
     CDS             complement(7460..7909)
                     /gene="kbp"
                     /locus_tag="J1Z28_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBA7718526.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(7993..8151)
                     /locus_tag="J1Z28_003730"
     CDS             complement(7993..8151)
                     /locus_tag="J1Z28_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBA7718527.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         1846 a         2165 c         2325 g         1899 t
ORIGIN      
        1 atatgtaact tttatgcgca tttttcagaa atgtagatat ttttagatta tggctacgaa
       61 atgagcatcg ccatgtcacc ctacatctca taagaggatc gcttctgatg aatgcactga
      121 ccgccgtaca aaataacgct gtcgattcag gccaggacta tagcggattc actctcatcc
      181 cgtcggcgca atccccgcgt ctgctggaac tcaccttcac cgaacagacg accaatcggt
      241 tcctcgaaca ggttgccgag tggcccgtac aggcgctgga gtacaaatcg tttctgcgtt
      301 ttcgggtagg caaaattctc gacgatctgt gtgcgaatca gctgcaaccg ctgctgttga
      361 agaccctgtt aaaccgcgct gaaggtgcgc tgttgatcaa tgcggtgggt gtcgatgatg
      421 tgaagcaagc ggatgagatg gtgaagctgg caacggcggt agcgcatctg attggtcgct
      481 caaatttcga tgcgatgagc ggtcagtatt acgcgcgatt cgtggtgaaa aatgtcgata
      541 actcagacag ctatctgcgt cagccgcacc gcgtaatgga gctgcacaac gacggcacct
      601 acgtcgaaga gatcactgac tatgtgctga tgatgaaaat cgacgagcag aacatgcagg
      661 gtggaaattc gttgctgctg catctcgatg actgggaaca tctggaccac tatttccgcc
      721 acccgctggc gcgtcgcccg atgcgctttg ccgcgccgcc gagcaaaaac gtcagcaaag
      781 atgttttcca tccagtgttc gacgttgatc aacagggtcg cccggtgatg cgttatatcg
      841 accagttcgt ccagccaaaa gacttcgaag aaggcgtgtg gctgagtgag ctttccgatg
      901 ccattgaaac cagcaaaggc attctttctg tgcccgttcc cgttggcaaa ttcctgttga
      961 ttaacaatct attctggctg cacggtcgcg accgctttac tccgcacccg gatctgcgcc
     1021 gtgaactgat gcgtcagcgt ggctatttcg cttacgccac tcaccactac cagacgcatc
     1081 agtaagcgca aaggaattga gcggatgtat gattttgtga ttattggcgg cggcatcatc
     1141 ggcatgtcga ccgccatgca actgattgat gtttatccgg atgcccgcat tgcgttgctg
     1201 gaaaaagagt ccggcccggc ctttcatcag acgggccaca acagcggcgt gatccatgcc
     1261 ggggtctatt acacgcccgg tagcctgaag gcacagtttt gcctggcggg aaaccgcgcc
     1321 accaaagcct tttgcgatca aaacggcatt cgctatgaca actgcggcaa gatgctggtc
     1381 gccacctctg aactcgaaat ggaacggatg cgcgcgttgt gggaacgcac ggcggcgaac
     1441 ggtatcgagc gcgagtggtt aaacgccgag gaactgcgcg agcgcgaacc gaatatcacc
     1501 gggcttggcg gcatttttgt gccgtccagc ggcattgtca gctatcgcga agtgacggcg
     1561 gcgatggcaa aaatcttcca ggccagaggc ggcgagatta tttataacgc cgaagtcagc
     1621 gccctcagtg agcataaaaa cggcgtggtg atacgtaccc gtcagggcgg tgaatatgaa
     1681 gcatcgacgc tgattagctg ttccgggctg atggctgacc ggctggtgaa aatgctcggc
     1741 ctcgaaccgg gctttattat ctgcccgttc cgtggcgagt atttccgcct tgcgccggag
     1801 cataaccaga ttgttaacca cctgatttac cccattcccg accccgcgat gccatttttg
     1861 ggcgtgcatc tcacccgcat gatcgatggc agcgtgaccg tcgggccaaa cgcggtgctg
     1921 gcttttaaac gcgaaggcta tcgcaagcgc gatttctcgt ttagtgacac gctggagatt
     1981 ttaggctcgt cggggattcg ccgggtgctg caaaaccatc tacgctcagg attgggcgag
     2041 atgaaaaact cgctgtgcaa aagcggctat ctgcggctgg tgcaaaagta ttgtccccgg
     2101 ctttcgttaa gcgatctcca gccctggccc gcaggtgtgc gggcgcaggc ggtatcgccg
     2161 gacggcaagc tgattgacga ttttctgttt gtcaccaccc cgcgcacgat ccacacctgc
     2221 aatgcgccct ccccggcagc gacatcagca attcctattg gtgcgcatat tgtcagcaag
     2281 gtacaaacgc tgttggcaag ccagagtaac cccggacgca cgctgcgagc ggcacgtagt
     2341 gtggatgcct tacacgccgc atttaatcaa taacctttga aaacaggacg taacaatgaa
     2401 acttaacgac agtaacttat tccgccagca ggcgttgatt aacggggaat ggctggacgc
     2461 caacaatggc gaggtcatcg acgtcaccaa tccggcgaac ggcgacaagc tgggtagcgt
     2521 gccgaaaatg ggcgctgatg aaacccgcgc cgctatcgac gccgccaacc gcgccctgcc
     2581 cgcctggcgt gcgctcaccg ccaaagaacg cgccaacatt ctgcgcaact ggttcaattt
     2641 gatgatggag catcaggacg atttagcgcg cctgatgacc ctcgaacagg gcaaaccact
     2701 ggccgaagcg aaaggcgaaa tcagctacgc agcctctttt attgagtggt ttgccgaaga
     2761 aggcaaacgt atttatggcg ataccattcc cggtcatcag gccgacaaac gcctgattgt
     2821 tatcaaacag ccgattggcg ttaccgccgc catcacgccg tggaacttcc cggcggcgat
     2881 gattacccgt aaagccggtc cggcgctggc ggcaggctgc acgatggtgc tgaaacctgc
     2941 cagtcagacg ccgttctctg cgctggcgct ggcggaactg gcgattcgcg caggcgttcc
     3001 ggctggcgta tttaacgtgg tcaccggttc ggcgggcgcg gtcggtaacg aactgaccag
     3061 caatccgctg gtgcgcaagc tgtcgtttac cggctcgacc gaaattggcc gccagttaat
     3121 ggaacagtgc gcgaaagaca tcaagaaagt gtcgctggag ctgggcggca acgcgccgtt
     3181 tatcgtcttt gacgatgccg acctcgacaa agccgtggaa ggcgcactgg cctcgaaatt
     3241 ccgcaacgcc gggcaaacct gcgtctgcgc caaccgttta tacgtgcagg acggcgtgta
     3301 tgaccgcttt gccgaaaaat tgcagcaggc ggtgagcaaa ctgcacatcg gcgacgggct
     3361 ggataacggc gtcaccatcg ggccgctgat cgatgaaaaa gcggtagcaa aagtggaaga
     3421 gcatattgcc gatgccctgg agaaaggcgc gcgcgtggtt tgcggtggta aagcacacga
     3481 acgcggcggc aacttcttcc agccgaccat tctggtggac gttccggcca acgccaaagt
     3541 gtcgaaagaa gagacgttcg gccccctcgc cccgctgttc cgctttaaag atgaagccga
     3601 tgtgattgcg caagccaatg acaccgagtt tggtcttgcc gcctatttct acgcccgtga
     3661 tttaagccgc gtcttccgcg tgggcgaagc gctggagtac ggcatcgtcg gcatcaatac
     3721 cgggataatt tccaatgaag tggccccgtt cggcggcatt aaagcctcgg gtctgggtcg
     3781 tgaaggttcg aagtatggca tcgaagatta cttagaaatc aaatatatgt gcatcggtct
     3841 ttaactggag aatgcgaatg agcagcaata aagagttaat gcagcgccgc agtcaggcga
     3901 ttccccgtgg cgttgggcaa attcacccga ttttcgctga ccgcgcggaa aactgccggg
     3961 tgtgggacgt tgaaggccgt gagtatcttg atttcgcggg cgggattgcg gtgctcaata
     4021 ccgggcacct gcatccgaaa gtggttgcgg cggtggaagc gcagttgaaa aaactgtcgc
     4081 acacctgctt ccaggtgctg gcctacgagc cgtatctgga gctgtgcgag attatgaatc
     4141 agaaggtgcc gggcgatttt gccaagaaaa ctctgctggt tacgaccggt tccgaagcgg
     4201 tggaaaatgc ggtgaaaatc gcccgcgccg ccaccaaacg tagcggtact atcgccttta
     4261 gcggtgcgta tcacgggcgc acgcattaca cgctggcgct gaccggtaag gtgaacccat
     4321 actctgcggg catggggctg atgccgggtc atgtttatcg cgcgctttat ccttgcccgc
     4381 tgcacggcat aagcgaggat gacgctatcg ccagcatcca ccggatcttc aaaaatgacg
     4441 ccgcgccgga agatatcgcc gccatcgtga ttgagcccgt tcagggcgaa ggcggtttct
     4501 acgccgcgac gccagccttt atgcagcgtt tacgcgcgct gtgtgacgag cacgggatca
     4561 tgctgattgc cgatgaagtg cagagcggcg cagggcgtac cggcacgctg tttgcgatgg
     4621 agcaaatggg cgtggcgccg gatctcacca cctttgcgaa atcgatcgca ggcggtttcc
     4681 cgctggcggg cgtcaccggg cgcgcggaag tgatggacgc cgtcgcaccg ggcggactgg
     4741 gtggcaccta tgccggtaac ccgattgcct gcgtagcggc gctggaagtg ttgaaggtgt
     4801 tcgagcagga aaatctgctg caaaaagcca acgttctggg gcagaagttg aaagacggat
     4861 tgctgtcgat agccgaaaaa cacccggaga tcggcgacgt acgcgggctg ggagcgatga
     4921 tcgccatcga gctgtttgaa gatggcgatc acagcaaacc ggacgcaaaa ctcaccgccg
     4981 agatcgtggc gcgtgcccgc gataaaggcc tgattcttct ctcctgcggc ccgtattaca
     5041 acgtgctgcg catccttgta ccgctcacca ttgaagacgc tcagatccgt cagggtctgg
     5101 agatcatcag ccagtgtttt gatgaggcga agcagtagat ccgtacccat gccggaggcg
     5161 acgctgcgcg tcttgtccgg cctacgcgaa tccaggtcgg ataaagcgtt tacgctgcat
     5221 ccgacaatct gtatgtgaac aggaataaat ctatgtcggc cggataaggc ggagccgctc
     5281 tccggtaaaa agaatcaata acaattacac gcgtgacccg acgcgggaaa tgtcggggcg
     5341 ctctcccaag tgacacactt tcgagaggat tcaggatggg gcaatcatcg caaccacatg
     5401 agttaggcgg cgggctgaag tcacgccacg tcaccatgtt gtctattgcc ggtgttatcg
     5461 gcgcaagtct gtttgtcggt tccagcgtcg ccatcgccga agcgggcccg gcggtattac
     5521 tggcctatct gttcgccgga ttactggtgg ttatgattat gcggatgttg gcggaaatgg
     5581 cggttgccac gcccgatacc ggttcgtttt ccacctatgc cgataaagcc attggccgct
     5641 gggcgggcta taccatcggc tggttgtact ggtggttttg ggtactggtt atcccacttg
     5701 aagccaacat cgccgccatg atcctgcact cgtgggttcc aggcattccc atctggttat
     5761 tttccctcgt cattaccctc gccttaaccg gcagtaattt attaagcgtt aaaaactacg
     5821 gcgaatttga gttctggctg gcgctgtgta aagtcatcgc tatcctggcc tttattttcc
     5881 ttggtgcagt cgcaattagc ggtttttacc cttatgccga agtgagcggg atctcaagat
     5941 tgtgggatag cggcggcttt atgcccaacg gcttcggtgc ggtattaagc gcgatgttga
     6001 tcaccatgtt ctcgtttatg ggcgcagaaa ttgtcactat tgccgccgcg gaatctgaca
     6061 cgccggaaaa acatattgtc cgcgccacca actcggttat ctggcgtatt tctatcttct
     6121 atttgtgctc tatttttgtc gtcgtggcat taataccgtg gaatatgccg gggctgaaag
     6181 ccgttggatc ttatcgctcg gttctggagt tgctcaatat tccccatgcg aaattaatca
     6241 tggactgcgt gatattactt tccgtaacca gctgcctgaa ctcggcgctg tataccgctt
     6301 ccagaatgct ctactcctta agccgtcgcg gtgatgctcc cgcggtaatg ggtaaaatca
     6361 accgcagtaa aaccccgtat gtggcggtat tactctccac cggcgcggca ttcttaacgg
     6421 tggtggtgaa ctattacgcg cctgcgaagg tatttaaatt tctgatcgac agctccggcg
     6481 ctatcgccct gctggtttat ttagtcatcg ccgtttcaca gttgcggatg cgcaaaattc
     6541 tgcgagcaga aggaagcgaa attcgcttgc gcatgtggct ttatccgtgg ctcacctggc
     6601 tggtcatcgg ctttattacc tttgtgttgg tagtgatgct attccgaccg gcgcaacagt
     6661 tagaagtgat ctccactggc ttattagcga tagggattat ctgtaccgtg ccaattatgg
     6721 ctcgctggaa aaagctggta ttgtggcaaa aaacacccgt tcataatacg cgctgataat
     6781 gatcaggagt cacaccatga ccattacgtc tctggatggc tatcgctggc tgaagaacga
     6841 tattattcgc ggtaattttc aaccggatga aaaattacgg atgagtttgc tgacatcgcg
     6901 ttatgcactt ggcgttgggc cgttacggga agcgctttcg caactggtgg cggagcggct
     6961 ggtcacggtg gtgaatcaaa aagggtatcg ggtggcgtct atgtcagagc aggagctgct
     7021 cgatattttc gacgcccgcg ccaatatgga agcgatgctg gtcagtctgg cgattgcccg
     7081 cggtggcgat gaatgggagg caaacgttct cgcaaaagcg catctgctca gtaagcttga
     7141 ggcctgcgac gccagtgaga aaatgcttga tgagtgggat ctgcgtcatc aggcgtttca
     7201 tactgcaatt gtggcgggct gtggttctta ctatttgctg caaatgcgtg aacggttgtt
     7261 tgatctggca gcgcgttatc gatttatctg gctgcggcga acagtgcttt cggtggaaat
     7321 gctggaagat aaacacgatc agcaccagac cctgactgcg gcggtactgg cgcgagatac
     7381 cgcgcgcgcc agtgagttaa tgcgccagca tttactgacg ccaattccca ttatccagca
     7441 ggcgatggct ggcaattaac tactcttcag gaatacgcaa cacttgcccc gggtaaattt
     7501 tatccgggct ttttagcatc ggtttattcg cttcgaagat tttattgtac agattagcgt
     7561 taccgtagac ctgtttggaa atggcactca gagtgtcgcc agacttaacg gtataaaact
     7621 ggctggcagt ggctggtgtc gccgttttca cctgatcatc gacactggca ataccggaaa
     7681 tattccccac cgcaacaagg attttctcct tcgcctcctg acttaagccg tcacccgtga
     7741 ccgtcgcttt gccgtcggca atttgaatat tcactttatc ggcatcaggt ataccggttt
     7801 tgttcagatg ctcctgcacc ttcttcgcct gatcgtcttt atcgtgctga cctgtaaccg
     7861 cgtcccagag tttttctccg gcatctttca caaaattgaa cagacccata gctacctcat
     7921 tcgttaacgg aaacactcag aaagtgtagc agaggaacgc ggcgcgttaa cgccgcgggg
     7981 gtgaacacag gtttaatcgc gggtttgcac ccagaacgcg tgaatcaaac cggggatata
     8041 cccaagcagt gtcagcagaa tattaataat gaacgcccaa ccgaaccctt taccgagcag
     8101 cacgccgagc ggcggcagaa tgatggtgat gacgattctc cagaaaccca tatgtactcc
     8161 ctataagaaa attactcatt gtttaaaaag agattttatc tcttaagcgt agtaatttta
     8221 gcggagcctg ccagt
//
LOCUS       DADSZD010000171         8153 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.172, whole genome shotgun sequence.
ACCESSION   DADSZD010000171 DADSZD010000000
VERSION     DADSZD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.172"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            220..4002
                     /locus_tag="J1Z28_003731"
     CDS             220..4002
                     /locus_tag="J1Z28_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718528.1"
                     /translation="MGRKSREKRERKLTEQKVDYDSLLHDMSRYRELFTNGVGEDTYF
                     CKHLEATQNLFRRYKTLDVAVALFTSELWPANTGSPIKHIFAWCVLLELPFESQVGIS
                     IDSYIDFKVFTKELYASWPEFPMLEDFSPEADWGQIKVRLGQDFVPMFYGSCIERMSD
                     FVEAFRITYAHIPEAQAHMDLAIALQAQIIESIPELMLSVNTEAQCADVELATEDFWL
                     TCKSLLQQLGNELANRRKKAGCTFDTLVGTFKAPLKPDAFGNAVMQGMALPFLAVEMD
                     GLWIPMSVRSAPGLIIDHWANKNLNGISPSTHRKLAEFVAERFQRTLMGPLTLYVDGT
                     ACKDLPISCIIPGNSGIYLICTCDHTSNDRLSNAAESIYAKVSCGSPIFFRLDDGRFL
                     SLSRDGAEPGVDELHIVVVVTLAGTAFNSINLPSKPTRLFPLADFITIFDSLKDLEEL
                     ESYWKFADAQEGTLSPFSCGTVDLFASFKDMHGVLVEGAIAPNFIGLDPHWGTSWRFK
                     ILTEFWSRAPKVFPDSSIGWALSSSTEGVVRLESRHHKAMAYSTSIGSCTVQTLIEIP
                     KELRFEDGRLIGLFAQLLADCSYRCRKMMSDISLFRQPHVLFSCSPDPSSLVKAEESP
                     SSLDEFKHVVTSVIPDSHNPDVFHVQVDTRAVLAGLNLSKDGSFEVRCLLETLEKCHA
                     VLGLQMPDEFAQRFTHIASQAARYHLKVMTRNIDVPDYVYPIIPSPTDYKLARRRLAA
                     EIMGLGFIPGRYELSDAKARIDPSSARMRLHIENRLAAFDRHQLLQAFIEQHDALLVT
                     ERTRIQRARLSLAHEVEYDRLDAIEQARKEFGDMARHYRYLLEKTVSLSSNGVDDVTE
                     DVLRELVGLVDWFMVLTGASDTLHNGIDVGGVEIDDSYIPEVFYSAGFDDREAVFARE
                     YAKSRLGLGANDKDAVEGESVDLLSSEKLKEAFITDLGFELQNMLTALAVLSQAQRYG
                     FGDELSLSYVAAPDRLAQVLSDSIDRLDYSEAQKIVAFLTLSETGVRRLAGRDIDEGD
                     IPYWERNKRIDRYTIKPLVIDGTDLRWGAESASRAMYIWMSAVRDGYLPAEFDWPHVK
                     PVIRDVKESIEKRLELRTEEIFRRHTPYVLRGIDFFRRFRGEKFDDVGGFDVFVYWPE
                     DNLLVTVECKYNQPPYTMKDSRRLRDRIFGKAENDRAGQFSRILDRRKFLETHRSRLL
                     ELLQWPQHESVPLRNIELYVSRDVYYWMVHPPYPVPTQFVRVDTLDTWIKTELNIP"
     gene            4007..>8153
                     /locus_tag="J1Z28_003732"
     CDS             4007..>8153
                     /locus_tag="J1Z28_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HBA7718529.1"
                     /translation="MSEDIKKTAITAAGYIYQNRQGLKVLCDWLDAPTRYSRVKFECD
                     NENEAPQGLDDIVIERSDGFVDLQQIKFTPDSDTHLLSWDWMLEKTGKTERSRSMLRK
                     WFDAFKQLDPARIGEISLITNRRPDAAIEACLGLDRIHFSKIPEPQRSRVETELGSIE
                     ECEAFFNQLRIRHSDKGYLALEHEVDARLRLHGTPEGIANLKYVALNWATQKNLPAPD
                     GWIKLAEVGTILRASPPAPLPEDFVVPEGYEVPDEIFHQEFVQSTINATGKAIVLTGP
                     PGRGKSTYLSALCDTLAEKGIPTVRHHYFLSTTERGRDRVNSYVVEQSILAQIQQFHE
                     DVPKTGGGLHTQLEACAAHYKVLGKPFVLILDGLDHVWRINAEDKRPLDDLFCQVIPC
                     PENMVLLVGTQPVNDEQLPADLLAFAPKSEWHTLPAMSESAVLSYLRRTVQEGWLTTG
                     FESEGQIEEQLQSAASALRKKTNGHPLHVIYATSELKHSGRRLSSWDIEQLKGDLSQD
                     ARFYYASLWERLAPSQKDALRLICAFTFFWPKTAFSEIATKIKAVEPDVDKVEHLLHS
                     SVAGLKVFHESLAVFVRATDGFESRINELMPVIADWLEHQAPTSLRVNWLWTVQAKLG
                     NPNNLITGLTRDWIMSRLKEGYSEALFEILLSEALSAALETANFSDAYRLAHLKARMV
                     DGSQFQMQDSDLARLISFTLTLTSEESVIRESIASRHETDILHVAALGLALLSRGDII
                     QAEICGEEAFHRFRALCQFSNKYNSRAGSDELTFFASAFTQLGVIADTSENLAWLVEK
                     NSPDIWLPRVQMLIQKGNLDDLMLLAASLPDGQKKNIISDACIRAAATAGVVVTERED
                     FDELVRTPFVAAIEAALIKTSTPLSQPIPVNWISDSYYKQKDNLATLTHHWLFGAVHL
                     NLCMIAEGQTNFEHIKAPVYEDREDITDYLNVLSGIGAQIAQRWWRGEFINFHELYEL
                     LEPVESRRFRQSYFRKSATEDFQAALHRIACDIHIGSVLLSGTSAATLSPATIDVALQ
                     CAWFDSASFRTQYASGSLIRMADEAAITFIGAQRLLLDADIQNETSVHLQIPLQLCAI
                     AIFHRLYSEARELCTQTWELTTGYGHRKDPTLSNTVDAIAYLVDEVPNEACRLLAKIA
                     PQIHHVLDYTDGKGTRHVLAEADRLLAKLKPSALVIKFEEHICVGDWALAEGSMQAYI
                     KQGIIEEWPLDALMRTGLHPEIRDALQQLVEDGCAAASERLRILDKHNGWDIGCLLRH
                     ENSSSDADNKPYEGDVTVFRPEQLEDLLKVLEPYYKERPKLLRTWYQHWESQKQGRNL
                     LVELDKILFTENNKWMREALQLSEFAFQTKRRSSGLTAAWKYLVHAQIRNGGWIGYME
                     NVDV"
BASE COUNT         2185 a         1746 c         1982 g         2240 t
ORIGIN      
        1 gaaataagaa aaatttatta tttatcaatt cattgggttt tatggttgca ataacataaa
       61 tgtaacagtt tgtcggatgt gtttgatagt gtgaggctat gcacttttgt gagggcggaa
      121 cagtaagtga ctcaagatat tcattatgat acgcattaat aatttctatg cttcgatacc
      181 gtatagactg tcaacgtatg catacaggga ggatatgaag tgggtaggaa gtcacgtgaa
      241 aagcgggaaa gaaaactaac agagcaaaag gttgattatg attctttgct acacgatatg
      301 tccagatatc gtgaattgtt tacgaatggg gtaggtgaag acacatactt ttgtaagcat
      361 ctggaagcca cccagaattt atttcgccga tacaaaaccc tcgacgtagc agttgcatta
      421 tttacttccg aactctggcc agccaataca ggtagcccca taaagcatat ttttgcttgg
      481 tgtgtccttt tagagctgcc atttgagtct caggtaggta tatcgattga tagctatata
      541 gattttaaag tatttacaaa agaattatac gcatcatggc ctgaattccc aatgcttgag
      601 gatttttccc ccgaggctga ttgggggcaa atcaaagtac ggcttggtca agacttcgtc
      661 cctatgttct acgggagttg cattgagaga atgtctgact tcgtagaagc atttcgcatt
      721 acctatgcgc atattccaga agcacaagcc catatggatc ttgccattgc gctgcaagcg
      781 cagatcatcg aatcgattcc tgagttgatg ctctctgtca acaccgaggc ccaatgtgct
      841 gatgttgaac tagctactga agacttctgg ttgacttgca agtctttgct tcaacaactt
      901 ggcaatgagc tagcgaaccg ccggaagaaa gcagggtgta cgttcgatac tcttgttggc
      961 accttcaaag caccgctgaa accggatgcg ttcggaaatg cagtgatgca aggcatggcg
     1021 ctacccttcc ttgctgtcga gatggatggg ttatggatac caatgtcagt acgcagtgct
     1081 ccgggtctca ttattgacca ttgggctaac aaaaatctga acgggattag cccctctaca
     1141 cacaggaaac ttgcagagtt cgtcgctgag cgattccaaa gaacccttat ggggcctttg
     1201 actctatatg ttgacggcac cgcctgcaaa gacctgccta tcagttgtat tataccgggt
     1261 aattctggga tatatctcat ttgcacatgt gaccacacat cgaatgaccg tctgtcaaac
     1321 gcagcggaaa gcatatacgc caaggtgagt tgtggatcgc cgattttctt ccgtcttgat
     1381 gatggacgtt tcctttctct atctagagat ggtgcagaac ctggcgtcga cgagcttcac
     1441 atcgttgttg tcgttacact cgcgggtact gcgtttaatt ccatcaatct cccctcaaag
     1501 ccaactcgtt tgtttccatt agcggatttc atcacgattt ttgatagtct caaagatctg
     1561 gaggaattag aaagttattg gaaatttgct gatgctcaag aggggacgct cagcccattt
     1621 tcctgtggaa cagtagactt gtttgcatca ttcaaagaca tgcatggtgt acttgtcgaa
     1681 ggtgcgattg cacctaactt cattgggcta gaccctcact ggggtacttc ttggcgcttc
     1741 aagatattga cagaattctg gtctcgcgca cccaaggtat ttccagacag ctctatcgga
     1801 tgggcgttga gttcttcaac tgaaggtgtc gttcgacttg aatcgcgtca tcacaaagcg
     1861 atggcatact caacatcgat tggttcctgt accgttcaaa cattaataga aatacccaaa
     1921 gaattacgct ttgaagatgg acgtttgata ggcctttttg cccaacttct ggccgactgt
     1981 agctatcgct gcagaaaaat gatgtctgat atctctctat ttaggcagcc gcatgttttg
     2041 ttttcctgct cccctgatcc atctagtctg gttaaagctg aagagagtcc ttcttcttta
     2101 gacgaattta aacacgtggt gacttcagtc atcccggatt cccacaaccc cgatgtattt
     2161 cacgttcaag ttgatacacg tgcagtgctt gccgggttaa atctttccaa ggatggatca
     2221 ttcgaggttc gttgcttact ggaaacgctt gaaaaatgtc atgcagtact tggtttgcag
     2281 atgcctgatg aatttgctca acgatttact catatagcgt cgcaagcggc tcgttatcat
     2341 ttaaaagtca tgacccgtaa tattgatgtc cccgactatg tataccctat aattccttcg
     2401 cctaccgatt acaagcttgc acgtagaaga ctggccgcag aaatcatggg gcttgggttt
     2461 attcctggca ggtatgaact gtcagatgcc aaggccagga ttgatccttc cagtgctcgc
     2521 atgcgtcttc acattgagaa tcgacttgca gcatttgata ggcatcagct tctgcaggca
     2581 ttcatcgagc aacacgatgc cctccttgtt actgagagga ctagaatcca acgagcccgg
     2641 ttgagtcttg ctcatgaagt tgagtacgac agacttgatg cgattgaaca ggcacgcaaa
     2701 gaattcggag atatggctcg gcattatcga tatctgttgg aaaagaccgt cagtttatct
     2761 tcaaacggtg tagatgacgt tacagaggat gtactacggg agttagtagg tttggttgat
     2821 tggttcatgg tgctgactgg tgccagtgat acccttcaca atggcataga tgtgggggga
     2881 gttgaaatcg acgactcgta tattccagaa gttttctatt cggcaggttt tgatgatcgt
     2941 gaggcggttt ttgcaaggga gtacgcaaaa tccagactgg gactcggtgc aaatgacaag
     3001 gatgcggtag aaggtgaatc ggtagattta ctgtcgtcag agaaactcaa agaggctttt
     3061 ataaccgatc tgggcttcga actgcagaac atgctcactg ctctcgctgt tttatcgcaa
     3121 gctcaacgat atgggtttgg cgacgaactt tcactgtctt atgttgctgc accggatcgc
     3181 ctcgcgcaag tattgtctga cagtatcgat agacttgact attctgaggc ccaaaaaatc
     3241 gttgcttttc tgactctttc ggaaacaggt gttcgcaggc ttgctggacg ggatatcgat
     3301 gagggagata tcccatactg ggagcgtaac aagcgaatcg accgatacac aattaagcca
     3361 ctggtaattg atggtaccga cctgcgttgg ggggccgaat cagcaagccg ggcaatgtat
     3421 atttggatgt cggcggtacg agacggttat ttgccagcag aatttgattg gcctcatgtt
     3481 aagcccgtca ttcgcgatgt taaggaaagc attgaaaagc gcctcgagct tcgtacagag
     3541 gagatttttc gcagacatac accatatgtt ctgcgaggta tcgatttctt tagaagattc
     3601 cgtggagaga aatttgatga cgttggcggc ttcgatgtct tcgtatactg gccggaagat
     3661 aatttgcttg tgacggtaga gtgcaagtac aaccagccgc catatacaat gaaagatagc
     3721 cgtcgtcttc gtgatcggat atttggcaag gcagagaatg acagggcagg acaattcagt
     3781 cgtattttag accgcagaaa atttctcgaa actcaccgtt caaggttgct tgaactgctc
     3841 cagtggccac agcatgagag tgtaccttta aggaatattg aactctatgt aagccgcgat
     3901 gtgtactact ggatggttca tccgccatat cctgttccga cccagtttgt acgtgtagac
     3961 actttggaca cttggatcaa aaccgaacta aacatccctt gatgctatga gtgaagatat
     4021 taagaaaacc gccatcactg cagctggtta tatttatcaa aatcgtcagg gccttaaggt
     4081 cctgtgcgat tggcttgatg caccgacgcg ctatagccgc gtgaaatttg aatgtgataa
     4141 tgaaaacgaa gcaccacagg gactagatga tatcgtcatc gaacgaagtg atggctttgt
     4201 tgaccttcaa cagatcaagt tcactccaga ttctgacacg catcttctat cttgggattg
     4261 gatgctagag aaaacgggca aaacggaacg gtctagatcg atgctacgta aatggttcga
     4321 tgctttcaaa cagctcgatc cagcacgtat tggcgaaatc tcgctaataa ctaaccgtcg
     4381 acctgatgca gcgattgaag cttgtctggg gctagatagg attcattttt ccaagatccc
     4441 tgagcctcag cgctccagag ttgaaactga actcggtagc attgaggaat gcgaagcgtt
     4501 tttcaaccaa ttacgcattc gacacagcga taagggctat cttgcattgg agcatgaagt
     4561 tgatgctcgc cttcgactgc atggtacgcc agagggaatc gctaatctaa aatatgtggc
     4621 actgaattgg gcgacgcaaa aaaatctccc tgcgccggat ggctggatca aactggcaga
     4681 ggttggaact atcttgcgag cttcacctcc agctcctttg ccagaggatt ttgttgttcc
     4741 agaaggttat gaggttcctg atgagatatt ccatcaagaa tttgtccaga gcacaattaa
     4801 tgctaccggg aaggcaattg tgctgactgg tccaccagga cgcggaaaga gtacttacct
     4861 gagcgcactg tgcgacacgc ttgcagaaaa aggtatccca acggttcgcc atcactattt
     4921 tctgtcgaca accgagcgag gccgcgaccg ggttaatagc tacgttgtcg agcaatccat
     4981 tctggcacag atccaacagt ttcacgagga tgttccgaag actggtggag gcttacatac
     5041 acaattggag gcatgtgctg cccactacaa agtactggga aagccatttg tgctcattct
     5101 tgatggtttg gatcatgtct ggcgtatcaa cgctgaggat aaacggccac tagatgacct
     5161 tttctgtcag gtaattccct gcccggaaaa tatggtgctg ctcgtcggca ctcaaccggt
     5221 aaatgatgaa cagttgccag cggatttgct cgcctttgca ccaaaatctg agtggcacac
     5281 attgcctgca atgtctgaga gtgcagtact ttcttatcta cgtagaacag ttcaggaagg
     5341 ctggcttaca actggttttg aaagtgaagg acaaattgaa gaacaactgc agagtgcagc
     5401 atctgctctt cgtaagaaaa ctaatggcca tcctcttcac gttatttatg caacttcaga
     5461 attgaaacat tcgggccgca gactatctag ttgggatatt gagcagttga agggagactt
     5521 gagccaagac gcccgatttt actatgcatc tctatgggaa cgtctcgctc caagccaaaa
     5581 ggatgcgcta aggcttatct gtgcctttac ttttttctgg cctaagacag ccttctcaga
     5641 gattgctacc aaaattaagg cggtggaacc agatgtggat aaagtagagc acttgctcca
     5701 ttcgtctgtg gctggtctta aagtcttcca tgaaagtttg gctgttttcg tacgtgcaac
     5761 tgatggcttt gagagtcgaa tcaatgaatt aatgcctgtt attgcagact ggcttgaaca
     5821 ccaggcacct acttcactac gagtgaactg gttatggaca gtccaagcga agcttggtaa
     5881 ccccaacaat cttattacag gtttaactcg tgactggatc atgagtcgct taaaagaagg
     5941 atattcagaa gctttatttg agatattatt gtcagaagcc ttgagcgctg cgcttgaaac
     6001 tgcaaatttt tcagatgcct accggttagc gcatctgaaa gctcgtatgg ttgatggtag
     6061 tcaatttcag atgcaggata gcgatctagc taggctcata tcttttacac tgacactaac
     6121 ctcagaggaa agtgtgatac gagagtctat agcctccagg catgaaacgg atattttaca
     6181 cgttgctgca cttggtctgg ccttactgtc tcgaggggat attattcagg cagaaatttg
     6241 cggtgaagaa gcctttcatc ggttcagagc cctatgccaa ttttccaata aatataattc
     6301 aagggctggt tctgatgaac tcacgttttt tgccagtgca tttacacagc ttggcgttat
     6361 cgctgataca tctgaaaatc tcgcttggtt agtcgagaaa aatagcccgg acatatggtt
     6421 acctcgcgtt caaatgctga ttcaaaaagg taatttggat gatttaatgt tgctcgcagc
     6481 ttctctaccc gacggacaga aaaagaacat aattagcgat gcttgtatac gtgcggctgc
     6541 gactgcagga gtggtagtga ccgagcgaga ggacttcgac gaacttgtca gaactccatt
     6601 tgtggctgca atagaggccg cgttaatcaa aacctcaact cctttgagcc aaccaatccc
     6661 ggtgaattgg ataagcgaca gctattacaa acaaaaagat aatttggcga ctctcacgca
     6721 tcactggcta tttggcgccg tacatctgaa tctttgtatg attgcagaag ggcaaacaaa
     6781 cttcgagcac atcaaagccc ctgtgtacga agatagagaa gatattactg attatctcaa
     6841 tgtactgtcc ggaattggtg cacaaatagc ccaacgatgg tggcgtgggg aattcattaa
     6901 cttccatgag ctgtatgaat tactcgagcc tgttgaatcg cgacgattta gacaaagtta
     6961 ttttagaaaa tcggcaacag aggactttca agcggcacta caccgtattg cttgcgatat
     7021 acatattggt agtgttctgc tttcgggaac tagtgctgcg acactttccc cagcgaccat
     7081 tgatgttgcg ttgcaatgcg cttggttcga tagtgcgtca ttccgcactc agtacgcatc
     7141 tggttcattg atcagaatgg ccgacgaagc ggctatcaca tttattggcg ctcaacgttt
     7201 gttacttgat gcagacattc aaaatgagac tagtgtacat cttcaaatac cattacaact
     7261 ctgtgcgata gcgatcttcc atcgactgta ttcggaggct cgtgagcttt gcacgcagac
     7321 gtgggaattg accactggat acggacatcg taaagacccg acactgagta atacggttga
     7381 tgccatcgct tatttggttg atgaagtgcc gaatgaagct tgtcgattac tggctaaaat
     7441 tgctccgcag atacatcatg ttttggatta caccgatggt aagggtacga gacatgttct
     7501 tgctgaagca gatcggctac tggcaaagct gaagccttcc gcattggtaa taaaatttga
     7561 agagcatata tgtgtgggtg actgggcttt ggctgagggc agcatgcagg cctacatcaa
     7621 acaaggaatc atcgaagagt ggcctctgga tgctctaatg cgcacaggcc ttcatcccga
     7681 aattcgggat gcactgcaac aacttgtaga ggatggttgt gcagctgctt ccgagcggct
     7741 tcgcattctt gataagcaca atggatggga tattggttgt ttactgcgcc acgaaaattc
     7801 aagtagtgat gctgacaaca aaccttatga aggtgatgtg acggtcttta gacctgagca
     7861 attagaagac ttgctcaaag ttctggaacc atattacaaa gagcgtccaa aacttctccg
     7921 tacttggtat cagcactggg aaagccaaaa gcaggggcga aatttactcg tcgaactgga
     7981 taagattcta tttacagaaa acaacaaatg gatgagggaa gctctacaac tttctgagtt
     8041 tgcctttcaa acaaaacgta gatcaagtgg tttaacagcg gcctggaagt atcttgttca
     8101 tgcacagatc cgtaatgggg gatggattgg ttacatggag aacgtggatg tga
//
LOCUS       DADSZD010000172         8126 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.173, whole genome shotgun sequence.
ACCESSION   DADSZD010000172 DADSZD010000000
VERSION     DADSZD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.173"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            7..3072
                     /gene="ag43"
                     /locus_tag="J1Z28_003733"
     CDS             7..3072
                     /gene="ag43"
                     /locus_tag="J1Z28_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBA7718530.1"
                     /translation="MVVASELARSRGKGAGVAVALSLAAVTSVPALAADSIVQAGETV
                     NGGTLENHDNQIVLGTANGMTISTGLELGPDSEENTGGQWIQNGGIAGNTTVTTNGRQ
                     VVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVHEGGVATGTIINRDGYQSVK
                     SGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTINKNGRQIILFSGIARDTLIY
                     AGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGWQVVKAGGAVGNTTVNQNGE
                     LRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSVVEGKADNVVLENGGRLDVL
                     TGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSGAAVSGTRSDGKAFSIGGG
                     QADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAGTTTLNNGAILTLSGKTVN
                     NDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLTQKAVNLNEGTLTLNDSTV
                     TTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIPDNATVQSVVDDLSHAGQI
                     HFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNADRLVIDGGRATGKTILNL
                     VNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQAGAFNYSLNRDSDESWYL
                     RSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNN
                     GGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAG
                     TVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETG
                     LPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTF
                     GEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQ
                     NGTSLDLQAGLEARVRENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            3193..5709
                     /locus_tag="J1Z28_003734"
     CDS             3193..5709
                     /locus_tag="J1Z28_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718531.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQYGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            5785..6240
                     /locus_tag="J1Z28_003735"
     CDS             5785..6240
                     /locus_tag="J1Z28_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718532.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            6319..6552
                     /locus_tag="J1Z28_003736"
     CDS             6319..6552
                     /locus_tag="J1Z28_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBA7718533.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            6652..7470
                     /locus_tag="J1Z28_003737"
     CDS             6652..7470
                     /locus_tag="J1Z28_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA7718534.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            7525..8010
                     /locus_tag="J1Z28_003738"
     CDS             7525..8010
                     /locus_tag="J1Z28_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA7718535.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            8026..>8126
                     /locus_tag="J1Z28_003739"
     CDS             8026..>8126
                     /locus_tag="J1Z28_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
                     /protein_id="HBA7718536.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPG"
BASE COUNT         1943 a         2154 c         2287 g         1742 t
ORIGIN      
        1 ggcaccctgg tggtggcctc cgaactggcc cgctcacggg gaaaaggcgc cggtgtggcg
       61 gttgctctgt ctcttgctgc tgtcacgtca gtcccggccc tggctgctga cagtattgta
      121 caggcaggtg aaaccgtgaa cggcggaaca ctggaaaatc atgacaacca gattgtcctc
      181 ggtacggcca acggaatgac catcagtacc gggctggaac tggggccgga cagtgaagaa
      241 aacaccggtg ggcaatggat acagaatggc gggatagccg gaaacaccac tgttaccaca
      301 aatggtcgtc aggtcgtgct ggagggggga acagccagtg atacggttat tcgtgacggc
      361 gggggacaga gcctgaacgg gctggcggtg aacaccacgc tgaataacag aggcgagcag
      421 tgggttcatg agggcggggt tgccaccggt acaattatca accgcgacgg ttaccagagc
      481 gttaaaagtg gcgggctggc aacaggaacc atcatcaaca ccggcgcaga aggcggcccg
      541 gattctgaca actcgtacac gggtcagaag gtccagggaa cagcagaatc caccaccatc
      601 aacaaaaatg gacggcagat tatcttattt tccgggatag cccgtgacac tctcatttac
      661 gcaggtggtg accagtcggt acacggaagg gccctgaata ccacactgaa tggcggttac
      721 cagtatgtgc acaaggacgg acttgcgctg aacacggtaa ttaacgaggg gggctggcag
      781 gttgttaagg caggtggtgc tgtcggtaac accaccgtta atcagaacgg tgaactgagg
      841 gtacatgccg gcggggaagc cactgcagtc acccagaaca cgggcggtgc actggttacc
      901 agtacggctg caaccgttac cggcataaac cgccttggag cattctcggt tgtggagggt
      961 aaagctgata atgtcgtact ggaaaatggc ggacgcctgg atgtgctgac cggacacaca
     1021 gccactaata cccgcgtgga tgatggcgga acgctggatg tccgcaacgg tggtaccgcc
     1081 accaccgtat ccatgggaaa tggcggtgta ctgctggccg attccggtgc cgctgtcagt
     1141 ggtacccgga gcgacggaaa ggcattcagt atcggaggcg gtcaggcgga tgccctgatg
     1201 ctggaaaaag gcagttcatt cacgctgaac gccggtgata cggccacgga taccacggta
     1261 aatggcggac tgttcaccgc caggggcggc acactggcgg gcaccaccac gctgaataac
     1321 ggcgccatac ttaccctttc cgggaagacg gtgaacaacg ataccctgac catccgtgaa
     1381 ggcgatgcac tcctgcaggg aggctctctc accggtaacg gcagcgtgga aaaatcagga
     1441 agtggcacac tcactgtcag caacaccaca ctcacccaga aagccgtcaa cctgaatgaa
     1501 ggcacgctga cgctgaacga cagtaccgtc accacggatg tcattgctca gcgcggtaca
     1561 gccctgaagc tgaccggcag cactgtgctg aacggtgcca ttgaccccac gaatgtcact
     1621 ctcgcctccg gtgccacctg gaatatcccc gataacgcca cggtgcagtc ggtggtggat
     1681 gacctcagcc atgccggaca gattcatttc acctccaccc gcacagggaa gttcgtaccg
     1741 gcaaccctga aagtgaaaaa cctgaacgga cagaatggca ccatcagcct gcgtgtacgc
     1801 ccagatatgg cacagaacaa tgctgacaga ctggtcattg acggcggcag ggcaaccgga
     1861 aaaaccatcc tgaacctggt gaacgccggc aacagtgcgt cggggctggc gaccagcggt
     1921 aagggtattc aggtggttga agccattaac ggtgccacca cggaggaagg ggcctttgtc
     1981 caggggaaca ggctgcaggc cggtgccttt aactactccc tcaaccggga cagtgatgag
     2041 agctggtatc tgcgcagtga aaatgcttat cgtgcagaag tccccctgta tgcctccatg
     2101 ctgacacagg caatggacta tgaccggatt gtggcaggct cccgcagcca tcagaccggt
     2161 gtaaatggtg aaaacaacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat
     2221 aacaatggcg gtattgcccg tggggccacg ccggaaagca gcggcagcta tggattcgtc
     2281 cgtctggagg gtgacctgat gagaacagag gttgccggta tgtctgtgac cgcgggggta
     2341 tatggtgctg ctggccattc ttccgttgat gttaaggatg atgacggctc ccgtgccggc
     2401 acggtccggg atgatgccgg cagcctgggc ggatacctga atctgataca caacgcctcc
     2461 ggactgtggg ctgacattgt ggccctggga acccgccaca gcatgaaagc gtcaacggac
     2521 aataacgact tccgcgcccg gggttggggc tggctgggtt cactggaaac cggtctgccc
     2581 ttcagtatca ctgacaacct gatgctggag ccacaactgc agtatacctg gcagggactt
     2641 tccctggatg acggtaagga caacgccggt tatgtgaagt tcgggcatgg cagtgcacaa
     2701 catgtgcgtg ccggtttccg tctgggcagc cacaacgata tgacctttgg cgaaggcacc
     2761 tcatcccgtg cccccctgcg tgacagtgca aaacacagtg tgagtgaatt accggtgaac
     2821 tggtgggtac agccttctgt tatccgcacc ttcagctccc ggggagatat gcgtgtgggg
     2881 acttccactg caggcagcgg gatgacgttc tctccctcac agaatggcac atcactggac
     2941 ctgcaggccg gactggaagc ccgtgtccgg gaaaatatca ccctgggcgt tcaggccggt
     3001 tatgcccaca gcgtcagcgg cagcagcgct gaagggtata acggtcaggc cacactgaat
     3061 gtgaccttct gacagaacca tcgcctctct gtggtcccgg tcatcatgac cgggacccgg
     3121 accggcgcaa cggatcttca acgccacatt cgctggcatt aacaataaca tgatattcat
     3181 cacggagtga ctatgttaca gatagtcggc gcgctgatcc tgctgatcgc aggatttgcc
     3241 attcttcgcc ttttgttcag agcattaatc agcacggctt ctgcgctggc agggctcata
     3301 ttgctgtgtc tgttcggccc ggccttactg gctggctata tcaccgaacg cataacccgg
     3361 ttattccata ttcgctggct ggcaggcgta tttctgacga ttgccggaat gatcatcagc
     3421 ttcatgtggg gacttgatgg taaacatatc gcgctggagg ctcacacctt tgactctgtg
     3481 aaatttattc tgaccaccgc tctcgccggt ggtctgctgg ctgttcccct gcagatcaaa
     3541 aacattcagc agtacgggat cacaccagaa gatatcagca aggaaattaa cgggtattac
     3601 tgctgttttt atactgcctt tttccttatg gcgtgttctg catgcgcacc attgatcgcg
     3661 ttacagtacg atatttcacc gtcactgatg tggtggggcg ggttgttgta ctggctggct
     3721 gcattagtga cgctgctatg ggcggccagc cagatccagg cgctgaaaaa actgacctgt
     3781 gccatcagcc agacactgga agaacaaccg gtgctcaaca gtaaatcgtg gctgaccagt
     3841 ttgcaaaacg attacagcct tcctgactca ctgacggagc gcatctggct gacgctcatt
     3901 tcacaacgga tttcccgggg agagctgagg gaatttgaac tggcagacgg aaactggtta
     3961 ctgaacaatg cctggtatga aagaaacatg gcagggttta acgaacagtt gaaagagaac
     4021 ctgtcattca cacctgatga actgaaaacg ctcttccgga accgcctgaa tttatcaccg
     4081 gaagcgaatg acgattttct cgatcgttgc ctggacggcg gtgactggta tcccttttca
     4141 gaaggtcgcc gttttgtatc attccatcac gtggatgagc ttcgtatctg tgcctcctgc
     4201 gggctgacag aagtacatca tgccccggaa aatcataagc cggatccgga atggtactgc
     4261 tcctctcttt gtcgcgaaac agaaacactg tgtcaggaaa tttatgaacg cccttacaac
     4321 agctttattt ccgatgcaac ggcgaatggt ctgattctca tgaaactgcc ggaaacctgg
     4381 agtacaaatg agaaaatgtt tgcttccgga gggcagggac atgggtttgc cgctgaacgg
     4441 ggaaaccata ttgtcgacag agtccgtctg aaaaacgcac ggatcctcgg tgataataat
     4501 gccagaaatg gagcagacag actggtcagc ggaacagaaa tccagacgaa atattgttca
     4561 actgcagccc gtagcgtcgg tgcggcattc gacggacaaa acggacagta tcgttacatg
     4621 ggaaataatg gccccatgca actggaagtc ccccgtgatc agtatgccgg cgctgttgaa
     4681 accatgagga ataagatccg cgaaggtaaa gtacccggtg taaccgatcc cgcagaagcg
     4741 tcccggctga ttcgtcgggg acatctgact tatactcagg cccgtaatat cacccggttc
     4801 gggaccatcg aatcggtcac ttatgatatt gccgagggtt cggttgtcag tctggcggcc
     4861 ggagggatca gttttgccct gacggcatcg gtcttctggc tcagcaccgg cgatcgcgat
     4921 gctgccctgc agacagctgc tgtccaggca ggaaaaacct tcacccgcac actggctgtc
     4981 tacgtcacaa cccagcaact ccaccggctc agtgttgttc agggtatgct gaagcacatt
     5041 gatttttcga ctgccagccc gactgtccga ctggctcttc agaaggggac cggtgcagga
     5101 aatatcagtg ccctgaacaa agtaatgaag ggtacgctgg tgacatctct ggcactggta
     5161 gctgtcacaa ccggccctga catgatcaaa atgttgcggg gacggatctc cggtgcgcag
     5221 ttcatcagga atcttgccgt ggcatcttcc ggtgtggcag gtggagctgt cgggtcagtg
     5281 gcgggcggga tactgttcag tccactggga ccatttggtg cactgacagg gcgtgtggtt
     5341 ggcggtgttc tggggggaat gattgcctcc gctgtatcag gaaaaattgc cggagcgctg
     5401 gttgaagaag atcgcgtcaa aattctggca atgattcagg agcaggtgac atggcttgcc
     5461 ggcagtttcc tgctgaccgg acatgagatt gaaaatctga acgagaatct ggcccgtgtt
     5521 atcgatcaga atgctctgga gatcattttc gccgccggta tacaacaacg ggccgcgacc
     5581 aatatgttaa tcaaaccact ggtggtcagt atcatcaggc aacgccccgt catggaatat
     5641 gatgcatccc atctcggcaa tatggttaac cgactggaag aagcattacc cccggagtta
     5701 ccggcataac ggctattctg cgccttttac gctttatcac ctgatttgtc agtgtcatcg
     5761 tcatgactca gagaggtaaa taccatgatt cacctgttca aaacctgcat gattaccgcc
     5821 ttcattctgg ggttaacgtg gtctgcccca ctccgggcac aggatcaacg ttacatcagt
     5881 atacgcaata cagatacgat atggctcccg ggaaatattt gtgcttacca gttccggctg
     5941 gataatggcg gaaacgatga aggatttggc cccctcacca tcactctgca actcaaagac
     6001 aaatatggtc agacgctggt gaccagaaaa atggaaacgg aagcttttgg tgacagtaat
     6061 gccacgcgaa ccacagacgc atttctggaa acggagtgcg tggaaaatgt cgccacaacc
     6121 gaaatcatta aagcaactga agaaagtaac ggccatcgtg tcagtctgcc gttatcggtt
     6181 ttcaatcccc aggactacca tccactgctg attaccgttt ccggaaaaaa cgttaactga
     6241 tccctgaagt cagtttcatg cttcagcctc tgtcacaacg cgtggcagag gctttttatt
     6301 tatcaggaga aaacagatat gccgggttac acagagtatg tcctggctga aggatctttc
     6361 tcatacgggc aggcggttgc tgtcataacc gcttaccgca atgtgtttat ccaggatgac
     6421 ccgggaatgc atttccgtcg ggttatccgc aacgcggaag gtcagcgacg ctggcgctgc
     6481 cggaattctg aacccgatgc cggaaaggtg cttaatacgc ggctcgccag tgacggtctt
     6541 ctcagacaat aaacgtcatt catttcaccc atcaggccgc gtcttctccg ggagacgtgg
     6601 ctttttcatt tattacacca gtaaatctta accaccgata aggagcaaat tatgcgacta
     6661 gccagtcgtt ttggttatgc gaaccagata cgccgtgacc gtccgctgac acacgaagaa
     6721 ctgatgcact atgtgccggg gattttcggg gaagataaac acacgtcccg aagccagaac
     6781 tatacgtaca tccccaccat caccgtactg gaaagcctgc agcgggaagg ctttcagcca
     6841 ttcttcgcct gccagacccg tgtgcgcgac ccgggccgcc ggggatacac aaaacacatg
     6901 ctgcgtctgc ggcgggccgg agagataaac ggagaacatg tccctgaaat tattctgctc
     6961 aactctcatg acggtacctc cagctaccag atgctgccgg gttacttcag gttcgtctgc
     7021 cagaacggat gcgtctgtgg tcagtctctg ggggaagtgc gtgttccgca ccggggaaat
     7081 gtggtggaca gagtcattga aggggcttac gaggtggtgg gcgtgtttga ccggattgag
     7141 gaaaagcgtg atgccatgca gtcgctgatt ctgccgccac cggcacgcca ggcgctggca
     7201 caggcggcac tgacttaccg ttatggtgac gaacatcagc ccgtcaccac cgccgacatt
     7261 ctgacgccac gacgccggga ggattacggt aaggacctgt ggagtgctta tcagaccatt
     7321 caggagaata tgctgaaagg cggaatttca ggtcgcagcg ctaaaggaaa acgtatccat
     7381 acccgtgcca ttcacagcat tgacactgac attaagctca accgcgcatt gtgggtgatg
     7441 gcggaaaccc tgctggagag cctgcgctga ttcagccatt accctgaaaa ccacgcattt
     7501 atttgtcctt taaggagtca tttcatgaaa accatttcac ataattccac cacatcttct
     7561 gtttccgtaa ccgctgcatc agggaatgac cagcctcagt tggttgccac catagtccct
     7621 gatgaacagc gcatcagctt ctggccgcag cattttggcc tcattccaca gtgggtgacc
     7681 ctggagcccc gtgttttcgg ctggatggac cgtctgtgcg aagactactg cggtggtatc
     7741 tggaatctgt acactctgaa caacggcggg gcatttatgg cccccgaacc ggatgacgat
     7801 gatgacgaaa catgggtact gttcaatgcc atgaacggca accgcgctga aatgagcccg
     7861 gaagccgctg gcattgctgc ctgtctgatg acgtacagtc atcacgcctg tcgtacggag
     7921 tgttatgcca tgacggtcca ttattaccgg ctgcgggatt acgccctgca gcatccggaa
     7981 tgcagcgcca ttatgcgcat catcgactga atggaggcag ggacaatgca gcagctttcc
     8041 tttctgcccg gagaaatgac gcccggcgag cgcagtctca ttctgcgggc tctgaaaacc
     8101 ctggaccgcc atcttcatga acccgg
//
LOCUS       DADSZD010000173         8103 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.174, whole genome shotgun sequence.
ACCESSION   DADSZD010000173 DADSZD010000000
VERSION     DADSZD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.174"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            2..1137
                     /locus_tag="J1Z28_003740"
                     /pseudo
     CDS             2..1137
                     /locus_tag="J1Z28_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1311..2081)
                     /gene="yafV"
                     /locus_tag="J1Z28_003741"
     CDS             complement(1311..2081)
                     /gene="yafV"
                     /locus_tag="J1Z28_003741"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBA7718537.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVSWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRISPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLW"
     gene            2235..2708
                     /gene="ivy"
                     /locus_tag="J1Z28_003742"
     CDS             2235..2708
                     /gene="ivy"
                     /locus_tag="J1Z28_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-lysozyme inhibitor"
                     /protein_id="HBA7718538.1"
                     /translation="MGRISPGGMMFKAITTVAALVIATSAMAQDDLTISSLAKGETTK
                     AAFNQMVQGHKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEK
                     SNQMTGLFSTIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2751..5195)
                     /locus_tag="J1Z28_003743"
     CDS             complement(2751..5195)
                     /locus_tag="J1Z28_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBA7718539.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            5435..6013
                     /gene="lpcA"
                     /locus_tag="J1Z28_003744"
     CDS             5435..6013
                     /gene="lpcA"
                     /locus_tag="J1Z28_003744"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBA7718540.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            6218..6985
                     /locus_tag="J1Z28_003745"
     CDS             6218..6985
                     /locus_tag="J1Z28_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBA7718541.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6956..7696)
                     /locus_tag="J1Z28_003746"
     CDS             complement(6956..7696)
                     /locus_tag="J1Z28_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBA7718542.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(7852..8055)
                     /locus_tag="J1Z28_003747"
     CDS             complement(7852..8055)
                     /locus_tag="J1Z28_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HBA7718543.1"
                     /translation="MNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWI
                     LIYKLTDKLLRFERTGTHAALFG"
BASE COUNT         2060 a         2072 c         2028 g         1943 t
ORIGIN      
        1 aatggaactt aaaaaattga tggaacatat ttctattatc cccgattaca gacaagcctg
       61 gaaagtggaa cataaattgt cagatattct actgttgact atttgtgccg ttatttctgg
      121 tgcagaaaat tgggaagata tagaggattt ttggtaaaca catctcgatt ttttgaagca
      181 atatggtgat tttgaaaatg gtattcctgt tcacgatacc atcgccagag ttgtatcctg
      241 tatcagcctg caaaatttca cgagtgcttt attaactgga tgcgtgactg ccattcttca
      301 gatgataaag acgttattgc aattgatgga aaaacgctcc ggcattctta tgacaagagt
      361 cgccgcaggg gagcgattca tgtcattagt gcgttctcaa caatgcacag tctggtcatc
      421 ggacagatca ggacggatga gaaatctaat gagattacag ctatcccaaa acttcttaac
      481 atgctggata ttaaagaaaa aatcatcaca actgatgcga tgggttgcca gaaagatatt
      541 gcagagaaga tacaaaaaca gggcggtgat tatttattcg ctgtaaaagg aaatcagggg
      601 cggcttaata aagcctttga ggaaaaattc ccactgaaag aattaaataa tccagagcat
      661 gacagttacg caattagtga aaagagtcac ggcagagaag aaatccgtct tcacattgtt
      721 tgcgatgtcc ctgatgaact tattgatttc acgtttgaat ggaaagggct gaagaaatta
      781 tgcgtggcag tctcctttcg gtccataata gcagaacaaa agaaaaagcc agaaatgacg
      841 gtcagatatt atatcagttc tgctgattta accgcagaga agttcgccac agcaatccga
      901 aaccactggc acgtggagaa taagctgcac tggcgtctgg acgtggtaat gaatgaagac
      961 gactgcaaaa taagaagagg aaacgccgca gaattatttt cagggctacg gcacatcgct
     1021 attaatattt tgacgaatga taaggtattc aaggcagggt taagacgtaa gatgcgaaaa
     1081 gcagccatgg acagaaacta cctggcgtca gtccttgcag ggagcgggct ttcgtaatct
     1141 tgctctggct ttattatcga attcggtgac cgccttgacg gtcacttctg agaaaggcat
     1201 ttacatagaa tggtaaacag gctcacagaa actacctggc atcagtcctt gcggggagcg
     1261 ggctttcgta gtcttccccg actctccccg gccctaaaca caacccccac tcaccacaac
     1321 ctaaactcat ccgcatcctg ccatgccgga aacttttctc tatattcccg caatgccatc
     1381 atcgatagct ccgcatcaat gcgcgttgcc tgatgcgcgt cggcagtagc gataatctcg
     1441 ccttgcggat taatcacccg gctgtcaccg cgataatggc agccgttgcc atcgctgccg
     1501 acgcgattgc atcccgccac atacgcttga ttctcaatcg cgcgggctgt cagcaatgcc
     1561 tgccagtgca gagagcgcgg agcaggccag ttggcgacgt acagggcgag gtcataatcg
     1621 ttgagattgc gcgaccacac cggaaaacgt aagtcgtagc acaccagcgg cgaaatacgc
     1681 cagccgcgcc attccacaat cactcgcgca ttgcccgctt tataatgtag atgctcatct
     1741 gccatgcgga acagatgacg cttatcataa aaatgtaccg tgccgcccgg ctcaaccagc
     1801 aaaaagcggt taaccgaacc agactccgtt tgtaatgcaa cactgcctgc aatcagcgca
     1861 ttgcactgct gcgctttggc tgtcatccag ctcactacgt catcttgtgc tagcgacgaa
     1921 gctgccgctt ccatggcaaa gccgctggta aacatctccg gtagaacgat cacatcgcgc
     1981 ccggtaatac cttccagttg acgatcaaaa tggcgcaggt tggcaggacc atccatccac
     2041 accagtggtt gctgcaaaag cgtaatcttc aaaccaggca cggtgtacaa ctcctttatg
     2101 cgaagggttt tataactcta acaccttatc aggcagttgc cttagcgcag aataaattga
     2161 taacaaatgc tgatattgga aatatctgat ttgcaaatta tcgtgttatc gccaggcttt
     2221 agggagttaa tcacatgggc aggataagcc cgggaggaat gatgtttaag gcaataacga
     2281 cagtcgccgc tctggtcatc gccaccagtg caatggcgca ggatgattta accattagca
     2341 gtcttgcaaa gggcgaaacc accaaagcgg cgtttaatca gatggtgcaa gggcataagc
     2401 tgcctgcctg ggtgatgaaa ggcggtactt atactcccgc acaaaccgtg actttgggag
     2461 atgagacgta tcaggtgatg agcgcgtgca aaccgcatga ctgtggctcg caacgtatcg
     2521 ctgtgatgtg gtccgagaaa tctaatcaga tgacggggct gttctcgact attgatgaga
     2581 aaacgtcgca agagaaactc acctggttga atgtgaacga tgcgctttcg attgatggca
     2641 aaacggtgct gttcgcggcg ttgaccggca gcctggaaaa ccatccggat ggctttaatt
     2701 ttaaataatt agccgataaa aaaacggagc cgaaaggctc cgttattcaa ttacgcggct
     2761 tcaactttcc gcactttctc cggcaacttt accggcttcg tcgccagctc ttctggatca
     2821 aagtcatcaa cgttaatact gcgtaaacgg ctttcttcag cttttaccag aatagcggct
     2881 tcatctttat caatcagccc cttcgccagc gcgttgtgcg ccagttcatc cagacgggta
     2941 aacggcaggt ttttacccaa ctctttacag atccgctgat gaatcgggtc ggcggcaatc
     3001 acatccacca gcgcctcttc cagtaaacca accgggttat gctcgcttgg cgtcaggtac
     3061 tgaccgcgac caatacgtga acgagtggcg ttcggtactt gtaaaatctt cgccacttta
     3121 tgatccagct tgtcagacgg tgccaggtaa tgacgtccgg tcgggaagat caccacattc
     3181 agcagcccgg caaccacacg gttcgggaag ttttgcagta aatcgtccat cgcctgttct
     3241 gcctgataca gcgcatcctg tacgccccag tgcaccagcg gcagatcggc ttcattacgg
     3301 ccttcgtcgt cataacgctt cagaacggca gaggcgaggt aaagctggct taaaatatcc
     3361 cccagacgtg ccgagatacg ctcacgacgt ttcaggctgc cgcccagcac cgccatcgag
     3421 acatcagaaa gcagggcgag gttggcgctc aggcggttca ggtgctgata gtagcgttta
     3481 gtggcatcgc cggttggtgt gctgctggtt aaaccgcgcg tcaggcccag ccagaagctg
     3541 cgaactttgt tgctaccgac gtgaccgata tgtttgaaca gcagtttatc gaacgcgtta
     3601 acgtcattgt tcttcgctgc ttccatctct tccagcacgt acggatggca acgaatcgct
     3661 ccttgtccga agatcatcat gctgcgggtc agaatgttag ccccttcaac ggtgatggca
     3721 atcggtgcgc cctggtaagc acgcgccagg aagttgcttt gcccgagcat aatgcctttg
     3781 ccaccagtga tatccatcgc atcaataatc gactgctgcc cgcggtgggt acagtgatac
     3841 ttaacgatag ccgacagcac ggcaggtttc tcaccgagca taatgccgta ggtaatcagc
     3901 gatgccgcag catccattac ataagcgttc ccggcaatac gagccagcgg ctcttcaatc
     3961 ccttccatct taccgataga gattttgaac tgacggcgaa tgtgcgcata cgcgcccgtt
     4021 gccagcgcta ccgatttcac gccgccggtt gagttggaag gcagggtgat gccgcggcct
     4081 accgagaggc actccaccag catccgccag ccttgcccgg ccatttttgg tccgccgata
     4141 atgtaatcga tcggtacgaa gacatcttta ccgcgcgtcg gtccgttctg gaacggtacg
     4201 ttcagcggga agtggcgacg accaatttcc acgcccggcg tggtggtcgg gatcagcgca
     4261 caggtaatac ctaaatcttc tgcaccgccg agtaatttct ccgggtcgga gagtttaaac
     4321 gccagaccaa gcacggtcgc aatcggtgcc agcgtaatgt agcgtttgtt ccaggtcaga
     4381 cgcattccca gcacctgctg gccctgccat tcgcccatgc agacaatccc ggtatccgga
     4441 atcgcgcccg catcggaacc cgcttcgggg ctggtcagtg caaagcaagg gatctcctga
     4501 ccacgcgcca gacgcggcag atagtgattt ttctgctcgt cagtgccgta atgttgcagc
     4561 agctcgcccg ggcctaatga gttcggcacg ccgacggtaa tcgccaggat cccgctcacg
     4621 ccggagagtt tttgcagcac gcgagactgg gcataagccg agaactccag cccgccgtac
     4681 tcttttttga tgatcatcgc gaagaaacga tgctctttaa ggtacgccca taactccggc
     4741 ggcaggtccg ccagctcatg agtaatctgg aaatcattcg ccatccggca ggcttcttct
     4801 accggaccgt cgagaaacgc ttgctcttca gcggtcaggc gcggctgcgg atagttatgc
     4861 agctttttcc agtccggctt gccctggaac aagtcgccct cccaccaggt ggtgcccgca
     4921 tcaatcgctt ctttctcagt gcgcgacatc ggcggcatca ccttacggaa accgcgaaat
     4981 accggtgcgg aaatcatcga cttacgcata ggcgcaaagt taaatggcac gaggataatg
     5041 gccagaggca ctagtaccca cgccgaccac agaccagcaa cgccgaggac ggctgtccag
     5101 gcgagcaaaa tcagactgct gataaataag ctcacgcggt gatagaacaa tgcgccgagc
     5161 aggacaaccg tagcgagaat actcaaaatc atcataacga aaagcccctt acttgtagga
     5221 ggtctgacca cttgtgatga tatggttgta gtggatgtaa aaacatttag caatgtgttt
     5281 acaatataat tacaacaaag ctcacattgt tgctgttttt atccgcactt caggtcaaaa
     5341 agtcctggtc atagcacctg cccgtacttc tcgcttttgg cggtatccgg tacactgcat
     5401 tttgtctatt acatttatgc tgaaggatat cctcatgtac caggatctta ttcgtaatga
     5461 actgaacgaa gcggcggaaa cgctggctaa ctttttaaaa gatgacgcca atattcacgc
     5521 cattcagcgc gcggcggtgc tgttagcaga cagctttaaa gccggtggca aagtgctttc
     5581 ctgtggcaac ggcggctccc attgcgacgc catgcacttt gccgaagagt tgaccggtcg
     5641 ctaccgtgaa aaccgtccgg gctacccggc gattgccatt tctgacgtta gtcatatttc
     5701 ctgcgtcggt aacgattttg gtttcaatga tattttctcc cgttacgttg aagcggtagg
     5761 tcgcgaaggc gatgtactgc tggggatctc cacttccggt aactctgcaa acgtgatcaa
     5821 agcgatcgcg gcggcgcgtg agaaggggat gaaagtgatc accctgaccg gtaaagacgg
     5881 cggcaaaatg gctggcacgg cggatattga aattcgcgtt ccgcactttg gttatgccga
     5941 ccgcattcag gagatccaca ttaaagtgat ccatatcctg atccagttaa ttgaaaaaga
     6001 gatggttaag taagtctggc gtaggccgga taaggcgttt acgccgcatc cggcatttgt
     6061 gctctgatgc ctgatgcgac gctgacgcgt cttatcatgc ctacaatctg cacccgaacc
     6121 gtaggccgaa taatgcgttc acgccacatc cgacctgaaa attcttaaat caatcttcgc
     6181 cgggggccat gcgctcccgc tgttgtggag gttacccatg tgcgaattgc tcgggatgag
     6241 tgccaatgtc cctaccgata tctgctttag tttcaccgga cttgtacagc gtggtggtgg
     6301 aaccgggcca cataaagatg gctggggcat taccttttac gaaggtaaag gctgtcgcac
     6361 atttaaagat ccgcaaccca gctttaattc ccccatcgcc aaacttgtcc aggactaccc
     6421 gataaaatcc tgttcggtgg tggctcatat tcgccaggct aatcggggcg aggtggcgct
     6481 ggaaaatact cacccgttta cccgcgagtt atgggggcgt aactggactt atgcccataa
     6541 tggacaactg acgggctaca aatcactgga aaccggcaac ttccgcccgg tcggtgaaac
     6601 cgatagcgaa aaagcctttt gctggctcct gcataaatta acgcagcgtt acccgcgcac
     6661 accgggcaac atggcggcag tgtttaaata tatcgcctca ctggcggatg aactgcggca
     6721 gaagggcgtt ttcaacatgc tactttcgga tgggcgctat gtaatggcgt attgctcgac
     6781 taatttacac tggatcaccc gccgcgcgcc gtttggcgtg gcaacgttgc tggatcagga
     6841 tgtggaaatc gacttcagct cgcagaccac accgaatgat gtggtcacgg tgattgcgac
     6901 acagccgctg acgggcaatg aaacctggca aaagattatg ccaggtgaat ggcgcttatt
     6961 ttgcctcggg gagcgtgtag tttgatgcca gttgtggctg cacaacttcg tggcttaacg
     7021 gcttgctgac cacgtaacgg ccattgacca cagaaacggt cggtggctta cgggtttgct
     7081 caaagtagtc gtagcccggc ttcagttgct cccagaagtc cttaaagttg gaatatttat
     7141 ggcgcttcat attggcgtcg gtcatgcgga acggataaat actcacttgc acgctcggct
     7201 gaccaaacac cagcgcacca gtaacgaact ggaagatctc atcaataccc tgattggtca
     7261 tcgcgtagca gccgatggaa acacaatcgc cgtgaatcat caggtatttc ccttcataac
     7321 catgcgcacg gtcataggca tttgggaatc caatattaat cgctttgtag taacggctgt
     7381 ctggttttaa ctgattacgc tggacgctat aaaacccttc cgggcttttg aaatcgccct
     7441 gacgctgttt tggccctaag ccgccggaat atttacagat tttatagctg tcgagcagtt
     7501 gatattgctc gcccattttg acgtagagat cgagcgtacg ttcttccttg aagatctgga
     7561 tgtagacagg ggatcccatc aactgctgct tatactcttt gctcactggc gtggtcgagc
     7621 tactgctgcc cagcaaaccg gcaaacgaaa cgcacgggat caacaacatc gcaagaatta
     7681 atgcgatttt acgcatactg cttattcctt gataaaacgg ttacacacgc caggacggca
     7741 aaatgaatcc caaatcggaa tagtctggat ttggaaggct cacattatca ccaaaagagt
     7801 tttacgcaag cctgtcggcg cagggtttac aaatttcatc ggcagcgggc tttacccaaa
     7861 tagcgccgcg tgagttccag ttctctcaaa tcgtaaaagt ttatcggtaa gtttgtaaat
     7921 caggatccag tccggctcga catgagcatc gcgataacct ttccatgaac cttgcagcgg
     7981 gtggtcttta taaacagctg gaagcggtaa agtattattg ataagaagcg tcataagata
     8041 tttcaattta ttcatatcct tatgacgctt ttgtgcaagt tttacatcct ttgaaaattg
     8101 tcc
//
LOCUS       DADSZD010000174         8049 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.175, whole genome shotgun sequence.
ACCESSION   DADSZD010000174 DADSZD010000000
VERSION     DADSZD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.175"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(76..954)
                     /gene="glxR"
                     /locus_tag="J1Z28_003748"
     CDS             complement(76..954)
                     /gene="glxR"
                     /locus_tag="J1Z28_003748"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBA7718544.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTDASDIIFIMVPDTPQVEDVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(1054..1830)
                     /gene="hyi"
                     /locus_tag="J1Z28_003749"
     CDS             complement(1054..1830)
                     /gene="hyi"
                     /locus_tag="J1Z28_003749"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBA7718545.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(1843..3624)
                     /gene="gcl"
                     /locus_tag="J1Z28_003750"
     CDS             complement(1843..3624)
                     /gene="gcl"
                     /locus_tag="J1Z28_003750"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBA7718546.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKVLMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(3714..4529)
                     /gene="allR"
                     /locus_tag="J1Z28_003751"
     CDS             complement(3714..4529)
                     /gene="allR"
                     /locus_tag="J1Z28_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBA7718547.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            complement(4607..5089)
                     /gene="allA"
                     /locus_tag="J1Z28_003752"
     CDS             complement(4607..5089)
                     /gene="allA"
                     /locus_tag="J1Z28_003752"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBA7718548.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            5319..6245
                     /gene="allS"
                     /locus_tag="J1Z28_003753"
     CDS             5319..6245
                     /gene="allS"
                     /locus_tag="J1Z28_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBA7718549.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIDVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSFCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            6314..7420
                     /gene="mnmH"
                     /locus_tag="J1Z28_003754"
     CDS             6314..7420
                     /gene="mnmH"
                     /locus_tag="J1Z28_003754"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBA7718550.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENMLAAEMLKTDASQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAYACGY"
     gene            complement(7472..8049)
                     /locus_tag="J1Z28_003755"
                     /pseudo
     CDS             complement(7472..8049)
                     /locus_tag="J1Z28_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395526.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
BASE COUNT         2054 a         2111 c         1901 g         1983 t
ORIGIN      
        1 catctttggc tgctttcttt ttttaatatt gctggccgat tcattttctg atatcggcca
       61 tttttattgc gggtatcagg ccagtttatg gttagccatt aattccagcg cctgcactaa
      121 tgcagagtga tccaactggc tgccaccgtt tgccgcacag gtattaaaca attcctggca
      181 ggtcgcggtg tttggcaggt tcagcgccag tgctttcgca ctttgcagtg ccaggttgag
      241 atctttctgg tgcagcgcaa ttttgaaacc tggattaaag gtgcgtttaa tcatgcgctc
      301 accgtgaact tccagaatac gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg
      361 taccgggtcc gcaccggctt ttgaagcaaa caacaaggct tcagaaaccg cttcaatatt
      421 gagcgccacg ataatctgat ttgccacttt gcaggtttga ccgtcgccgt taccgcccac
      481 gagagtgata tttttaccaa gcagttcaaa cagcggtttt acacgttcaa atactgcttc
      541 atcaccgcca accataatcg ataacgtccc ttcacgcgcg ccgatttcac cgccggagac
      601 tggcgcatcg agataatcgc cgcccagttc attcacctga cgagcgaaac gtttagtttc
      661 aatcggggaa atggagctca tatcaacaat ggttttgccc ttcagcgagg ctttggtaca
      721 accattttca ccaaacagaa cgtcttcaac ctgtggcgta tccggcacca taataaaaat
      781 gatgtccgat gcatctgtta cctggcgcgc agtttcaaca ctaacggcgc ccagtgacag
      841 taattcatca gcaaccggac caatggttgt gacatgcagt tgatgaccgg cacgcgccag
      901 attaatggcc atcggagtac ccataatgcc taagccaata aatcccagtt tcataattaa
      961 cctcttttaa atttcgcttt tcctgaattc agacaacacg atcccctgcc gacttagcga
     1021 cctaaaaaag cattgtctga atagcgttac gttttaacgg tacggatcca tccagcgtaa
     1081 accagcttcc gtggtggttt ggggtttata ttcacagcca acccaaccgt tgtagtcaga
     1141 attttcaatt accgtaaaga gatattcgta attaatttct ccggttcccg gttcgccgcg
     1201 atgcggatta tcagcgattt gcaggtgacc aattttatcg gcccattgcg tcatagtgtt
     1261 ggttaattca ccttccatcc gctgcatgtg ataaatgtca tactgaattt tcaaattgca
     1321 gcaaccaaca tcatcaatca atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg
     1381 aatatcaaaa tggttgatag gttcaatcag taataaaata tcttctttca tcagcatatt
     1441 cgcggcataa cgcaggtttt ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc
     1501 cggcgtttta ccgaccagac agttaatttt tttattaccc agcgcacgag cataacgaat
     1561 cgctgctgct acgccatccc gaaactcttc ttcacggcca ggaatacagg caataccccg
     1621 ctcgcccgcc gcccagtcac ccgctggtaa attgtgcagc gtatgctcga gtttattact
     1681 cgccagcacc tgttttaatt cttcaatgtc gtagtcataa gggaacataa attcaacgcc
     1741 gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga aaatcatatt caccaaataa
     1801 catcgataaa ttagcagaga aacgtaacat tatttatctc ccttattcat agtgcatgaa
     1861 acaggtttca gtcggtgcat ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt
     1921 atccagttca ctgcccatcg aaatattagt cacacgctcg agaataactt ccacgactac
     1981 cggtacccga tattgcgcca ttaagacttt tgcctgttca aaggctggtg caatatcttc
     2041 cggtttgaaa acgcgaattg ctttacaacc taaaccttcc gctactttta cgtggtcgac
     2101 gccataaccg ttcacttcgc tggagttaat attttcgaac gcgagctgta cgcagtaatc
     2161 catatcaaag gcacgttgcg actggcgaat cagacccaga taggcgttat tgaccagtac
     2221 atggatgtac ggaatgttga actgtgcgcc aacggctaat tcttcaatca ggaactggaa
     2281 gtcgaagtca ccggaaatcg ccaccacatt gcgctctgga tcagcggcac aaacccccag
     2341 cgccgccgga atcgtccagc ccagcgggcc tgcctgccca cagttgatcc agtggcggtc
     2401 tttaaagaca tgcagcattt gtgccgcagc gatttgtgac aggccaatgg tggtgacata
     2461 acaaacatcg cggccaaagg ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg
     2521 cacgttgtcg aagtgggttt tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac
     2581 ccactcttta cgacacggca gacgaccggc tttttgcatc tcctgcgcca cttcaaccag
     2641 cagcgtcagc gccgctttag catcagagac aatgccaaga tccggacaca gtacgcggcc
     2701 aatttgcgtc ggctcaatat cgatatgaac gattttgcgc ccttcggtgt atttctctac
     2761 cgaaccggta tgacggttgg caaaacggtt accgatacca aataccatgt cggaagccag
     2821 cagcgttgcg ttaccgtaac gatgcgcggt ttgcagaccc accatcccgg ccatcagttc
     2881 gtggtcatcc gggatacaac cccagcccat cagcgttggg atcaccggaa cgctggtcag
     2941 ttcagcaaac tgttgtaaca gtgcagctgc gtcagcattg attaccccgc ctccggcaac
     3001 aatcaccgga cgttcggcct ggattaacat ttctacagct ttttcgatct gcatacggct
     3061 ggcagcaggt ttgtagaccg gcagcggttc gtacatatca ggatcaaact cgatttccgc
     3121 aacctgtacg tcgaacggta aatccaccag taccggaccc ggtcgaccag aacgcatcag
     3181 gtgaaacgcc tgttgcagca cacgaggcac cagcgccgct tcacgaactg taaccgccat
     3241 tttgctgacc ggtttagcaa ttgcttcaat atcaacggcc tggaaatctt ctttatgcag
     3301 acgggcgcgc ggagcctggc cggtaataca cagaatagga atggaatcag cagatgcgga
     3361 atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa gtccccagac atacgccgat
     3421 attccctgcc gttgcgcggg tataaccttc cgccatgtgc gaagcacctt ccacatgacg
     3481 cgccagaatg tgacgaatac cgccgtgctt acgcatcgct gagtagaacg gattgattgc
     3541 agctcccgga acgccgaagg cggtggtgat accttctttc tccagcacat acattgccgc
     3601 gtcaacggct ctcatttttg ccattttatt cctacctcta ttaattggaa aatttttcca
     3661 actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg gatacgacag acactataga
     3721 tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc
     3781 tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc cggagatgga gatagcggca
     3841 acaacactac ctacatcatc gtaaattgct gaagctatgc aattcaggcc gacaacatgc
     3901 tcttctttat ctacggtata gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg
     3961 ggcatatcca caagcgtcgt tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc
     4021 atcaactctt cttccgccag cggataaagc agcgccttgc ccgcaccgga agcatgcagc
     4081 ggcagacgac tgcccagcgg cgcacacatt ctgaccatcg atttacactc taactgacca
     4141 attaataccg cttcattgcc gttacggatc gcgacattga ccgtttcgcc ggaaagtaac
     4201 atcaggcggc gcataaacgg cccggcgacg gagaggacat cgcggttatg gatgtacgct
     4261 gcaccgacgt taaagacacc taatcctata tgccaccagc ctaactgact gtcctgatag
     4321 acaaaatccg ctgcctgtaa aaccttcagc aagcgaaagg tcgtggagag cggcaaatcc
     4381 agattgagag agatatcgct aaccgacgaa cttcccccac ttttttccag atattgcaga
     4441 atcgcaattc cccgctctaa cgcctgtgca cccttctgtg ccacaggctc cgcctgtcct
     4501 ggcctgccgc gccgtctaac ttccgtcata cctacgttaa ctccgaccta caaatgagca
     4561 taaacaatga acaattcgtt tatcttatgc aagtcggttg caggcgtcac gcaaaacaga
     4621 gttcctgttc aggaatactt tcaacatcac agttgtcact gccgccgcga tcgatggtca
     4681 gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg ccagacgtta cgatggtaat
     4741 tcactccctg ttcgccattg gtaataaacg cccgcagggt tgacaggtct ggtttgtcgt
     4801 cacctaacgc caccaccacc acaaacactt cacctttcat cgggataaag gcctgtgtac
     4861 ccagcggatg acgttcgagt tcgtgaatag tcagcggcag attcgccggt tgcgcgcggt
     4921 taatgctgat aagcgtacgg tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac
     4981 gctccaccag tccattgtta atatggaaaa aatcccgttt ctgcgtttcg attacgtcgc
     5041 cataagcact aaaggcttcc tgacttaacg gtaatacctg aagtttcatc tttttgtcct
     5101 tgtaacataa ttccgttcaa ccaccgccga ccggtcagct tggtttcaat aacaggcaat
     5161 ctaaaacctt aagagaacaa catcaacaca aatgttggaa tatttttcca aaactagatt
     5221 tagtccacaa attgaacagg gttatcatgt aaaaatactg ataaatcaga ggataagaaa
     5281 atcacgcaac ttgcggattt aaaaggggat acctgaagat gttcgatcca gaaaccttgc
     5341 ggactttcat tgcggttgct gaaacaggaa gtttttcaaa agcggcagaa cgattatgta
     5401 aaaccacggc gacgatcagt tatcgcatta aacttctgga agagaatacc ggagtggcgc
     5461 tgtttttccg cacgactcgc agcgtgacgt tgacggcggc tggcgagcat ctactttgtc
     5521 aggccagaga ctggctgagc tggctggaaa gtatgcccag cgagttacaa caggtgaatg
     5581 atggcgtgga acgccaggtg aatatcgtca tcaacaacct gctctataac cctcaggccg
     5641 ttgccaggct gctggcgtgg ctaaatgaac gttacccgtt tacccaattt cacatctccc
     5701 gacaaatcta tatgggcgtc tgggactcgc tattgtacga aggtttttca ctggctatcg
     5761 acgtcacggg aaccgaggcg ctggcaaata cctttagtct tgatccctta ggatcggtac
     5821 aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa cgttgaagag ccgctaacag
     5881 aagcacagtt gcggcgcttt ccggcggtca atattgaaga cagcgcccgc accttaacca
     5941 aacgcgtcgc ctggcgattg ccggggcaaa aagagattat tgttcccgat atggaaacga
     6001 aaatcgccgc ccatctggcg ggcgttggca ttggtttttt gccaaaatca ttttgccagt
     6061 caatgatcga taatcaacaa ctggtcagcc gggtaatccc aacgatgcgc cctccttcgc
     6121 cattgagtct ggcatggcgc aaatttggca gcggcaaagc ggtagaagat attgtgacct
     6181 tgtttaccca gcgcaggccg gaaatcagcg gatttttaga aattttcggc aacccacgca
     6241 gttaaaaatc aagtttcctt gcgcgttggt gcgtaaaata gcgcgcaagc tgatttgagg
     6301 ttcacttttt actatgcaag agagacacac ggaacaggac tatcgtgccc tgctgattgc
     6361 tgatacgccc attattgatg ttcgcgcccc catcgagttt gagcaaggcg caatgcccgc
     6421 cgctatcaac ctaccgttaa tgaataacga tgaacgcgcc gccgttggca tctgctataa
     6481 acagcaaggg tcagacgcgg cgctggcact ggggcataaa ctggtggcgg gtgaaattcg
     6541 tcagcagcgc atggacgcct ggcgggcagc gtgcctgcaa aatccgcacg gtattctctg
     6601 ctgcgcccgt ggcggtcagc gctcgcatat tgtgcaacgc tggctgcatg acgcggggat
     6661 tgattatccg ctggtggaag gcggttataa ggcactgcgc cagaccgcga ttcaggcaac
     6721 cattgaactg gcacaaaaac cgatagtgct gattggcggt tgtaccggca gcggcaaaac
     6781 gctgttagta caacaacagc cgaacggtgt tgatctggaa gggctggcgc gccatcgcgg
     6841 ttctgctttt ggtcgcacgt tacaaccaca acttagccag gcgagttttg aaaacatgct
     6901 ggctgccgaa atgctaaaaa ccgacgccag tcagaatttg cgcttgtggg tgctggaaga
     6961 tgaaagccgg atgatcggtt cgaatcacct gccggaatgt ctgcgcgaac gaatgactca
     7021 ggcgacgatt gcggtggtag aagatccgtt tgagatccgt cttgagcgcc tgaacgaaga
     7081 gtatttcttg cgtatgcatc atgattttac ccacgcgtac ggcgacgaac agggctggca
     7141 ggaatattgc gaatacctgc atcacggact ttcggctatt aagcgtcggc tggggctaca
     7201 gcgatataac gaactggctg caaggctgga tgcagcactg acaacgcaac tcaccaccgg
     7261 cagcaccgac ggtcatctgg cctggctggt gccgttactt gaagaatatt acgacccgat
     7321 gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga
     7381 agtagcggaa tgggttaagg cttatgcctg cggttattaa cccacaaaaa tgcttttccg
     7441 tgcctcttta agaaattgtg caactggtta actaccagtt gccataggga tattttttat
     7501 ctttgataaa ctcaagaaca tattcatcgt ctccaagaga aaaatcatgc cgggaaattc
     7561 ctttacacat ttccattttt tcacctgaaa ccgtttctat cagaatctgc ttatccgtca
     7621 ggcgaatatt gggcttgtca ggtttctcca gccatacccg tacttttcct tccggagcca
     7681 gaccgattaa cagataccgc agataatact tttcactgcc accgcctggt ggtgaataag
     7741 gtgtgagcat caatttccag acttctcccg ccagcgtgat ccaggtctca tacacttttt
     7801 tatcaatcac tgaatcccag caaaaatgga tagacatagg gggatttttg gctttattga
     7861 atgccgtcat tccaccacta agattttcct gccattgtcc tattgttgtt gagacaggga
     7921 tagtgctatc cagggttctg aactgataat agatgccatc ggtatctaat aactccacat
     7981 agataacttc tgctggtagg gaattagggt gaaaaaattg aaatgccatt catcataggg
     8041 tgcaagcag
//
LOCUS       DADSZD010000175         8048 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.176, whole genome shotgun sequence.
ACCESSION   DADSZD010000175 DADSZD010000000
VERSION     DADSZD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.176"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            167..967
                     /locus_tag="J1Z28_003756"
     CDS             167..967
                     /locus_tag="J1Z28_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBA7718551.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFHRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            1164..2603
                     /gene="aldA"
                     /locus_tag="J1Z28_003757"
     CDS             1164..2603
                     /gene="aldA"
                     /locus_tag="J1Z28_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBA7718552.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERAAEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADIELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKVIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2644..3645)
                     /gene="gap"
                     /locus_tag="J1Z28_003758"
     CDS             complement(2644..3645)
                     /gene="gap"
                     /locus_tag="J1Z28_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBA7718553.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDIVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATTDNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            3834..4364
                     /gene="cybB"
                     /locus_tag="J1Z28_003759"
     CDS             3834..4364
                     /gene="cybB"
                     /locus_tag="J1Z28_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBA7718554.1"
                     /translation="MENKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4609..4782
                     /locus_tag="J1Z28_003760"
     CDS             4609..4782
                     /locus_tag="J1Z28_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718555.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4854..5003)
                     /gene="hokB"
                     /locus_tag="J1Z28_003761"
     CDS             complement(4854..5003)
                     /gene="hokB"
                     /locus_tag="J1Z28_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBA7718556.1"
                     /translation="MKRNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            5402..7042
                     /gene="trg"
                     /locus_tag="J1Z28_003762"
     CDS             5402..7042
                     /gene="trg"
                     /locus_tag="J1Z28_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBA7718557.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFAYILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQMVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASTAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(7080..8003)
                     /locus_tag="J1Z28_003763"
     CDS             complement(7080..8003)
                     /locus_tag="J1Z28_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7718558.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
BASE COUNT         2072 a         1866 c         2055 g         2055 t
ORIGIN      
        1 caattctctg ctgcaagatg aataatgttt atctacagca tttccttaaa aaatatgtca
       61 ggcttgcgga gtggcagtta aggaaatacg acttcctcct ttcagagtgc tccgcttctc
      121 actattatct cacgcagtat tcttaaggga acgataagga ggaaccatga atattacccc
      181 gtttccgacg ctttcgccgg caaccataga tgccataaat gttatcggac agtggctggc
      241 gcaggatgat ttctccggtg aggtgccgta tcaggccgat tgcgtgatcc ttgcaggcaa
      301 tgcggttatg ccgactatcg atgcggcatg taagattgcc cgcgatcagc aaattccttt
      361 actgattagt ggtggtatcg gtcactcgac aacttttttg tatagcgcca tcgcacagca
      421 tccacactac aacactatcc gcaccactgg cagagctgaa gctaccatcc tggcggatat
      481 cgctcatcag ttctggcata ttccgcatga aaaaatctgg attgaagacc agtcaacaaa
      541 ctgcggtgaa aacgcacgct ttagcatcgc gctattgaat caggccgtag aacgagttca
      601 tacggctatc gttgttcagg accccaccat gcagcggcgc acgatggcga cgttccaccg
      661 tataaccggg gataatcccg atgcaccacg ctggttaagt tatcccggat tcgttcctca
      721 gttaggaaat aacgcagaca gtgtaatctt tgttaatccg ttgcaaggat tatggccggt
      781 tgagcgttat ctatcgctac tcactggcga gctgccgcgt ttacgcgatg atagcgatgg
      841 ctacggcccc cgcgggcgag attttatcgt tcacgttgat tttccggcag aagtcattca
      901 ggcatggcaa acgctgaaac atgatgcggt gctcatcgag gcgatggaaa gtcgctcgtt
      961 acgttaaaaa ttgcccgttt gtgaaccact tgtttgcaaa cgggcatgac ttctgacttt
     1021 tatttctgcc tttaattcct tttacacttg tttttatgaa gcccttcaca gaattgtcct
     1081 ttcacgatgc cgtctctctg ataattgatg ttaattaaca ataaatttac caaaaacaaa
     1141 catgtaaatc acaggagtcg cccatgtcag tacccgttca acatcctatg tatatcgatg
     1201 gacagtttgt tacctggcgt ggagacgcat ggattgatgt ggtaaaccct gctacagagg
     1261 ctgtcatttc tcgcattccc gatggtcagg ccgaggatgc ccgtaaggca atcgatgcag
     1321 cagaacgtgc acaaccagaa tgggaagcgt tgcctgctat tgaacgcgcc agttggttgc
     1381 gcaaaatctc cgccgggatc cgggaacgtg ccgctgaaat cagtgcgctg attgtcgaag
     1441 aagggggcaa gatccagcag ttggctgaag tcgaagtggc ttttactgcg gactatatcg
     1501 attatatggc ggagtgggca cggcgttacg agggcgagat tattcaaagc gatcgtccag
     1561 gagaaaatat ccttctgttt aaacgtgcgc ttggcgtgac taccggcatt ctgccgtgga
     1621 actttccgtt cttcctcatt gcccgcaaaa tggctcccgc tcttttgacc ggtaatacca
     1681 tcgtcattaa acccagtgaa tttacgccaa acaatgcgat tgcattcgcc aaaatcgttg
     1741 atgaaatagg ccttccgcgc ggcgtgttta accttgtact cgggcgtggt gaaaccgttg
     1801 ggcaagaact ggcgggtaac ccaaaggtcg caatggtcag tatgacaggc agcgtctctg
     1861 caggtgaaaa gattatggcg actgcggcga aaaacatcac caaagtgtgc ctggaattgg
     1921 ggggtaaagc accggctatc gtaatggacg atgccgatat tgaactggca gtcaaagcca
     1981 tcgttgattc acgcgtcatt aatagtgggc aagtgtgtaa ctgtgcagaa cgtgtttatg
     2041 tacagaaaag catttatgat cagttcgtca atcggctggg ggaagcgatg caggcggttc
     2101 aatttggtaa ccccgctgaa cgcaacgaca ttgcgatggg accgttgatt aacgccgcgg
     2161 cgctggaaag ggttgagcaa aaagtggcgc gcgcagtaga agaaggggcg agagtggcgt
     2221 tgggtggcaa agcggtagag gggaaaggat attattatcc gccgacattg ctgctggatg
     2281 ttcgccagga aatgtcgatt atgcatgagg aaacctttgg cccggttctg ccggtggtcg
     2341 catttgacac gctggaagag gctatctcaa tggcgaatga tagtgattac ggcctgacct
     2401 catcaatcta tacccaaaat ctgaacgtcg cgatgaaagt cattaaaggg ctgaagtttg
     2461 gtgaaactta catcaaccgt gaaaacttcg aagctatgca aggcttccac gccggatggc
     2521 gtaaatccgg tattggcggc gcagatggca aacatggctt gcatgaatac ctgcagaccc
     2581 aggtggttta tttacagtcg taatgaatga aagaggcgga ggtttttcct ccgcctgtgc
     2641 tggtcagagt ttggcgaatt tttccagggt acgaatgagt tgagtgacga aaccatactc
     2701 gttatcgtac caggcgacgg tcttcaccag ttgtaaatcg cccactgcgg tgatttccgt
     2761 ttgggtcgca tcaaacaccg aaccgaaatg gctgccaatg atatcggaag agacaatttc
     2821 ttcgtcggta taaccaaacg attcgttatc ggttgttgct tttttaagtg cgttattcac
     2881 ctcttcagca gtcacttttt tccccaggat agacaccagc tcggtcactg aacctgtttt
     2941 cactggcacg cgttgcgcat ggcctttaag tttgccgcta agttcgggga tcaccagacc
     3001 aatggctttt gccgcacctg tagtgtgggg aatgatattt tctgccgctg cacgtgaagc
     3061 gcgtaaatct ttacctcgcg gaccatccac cagtgactga gtgccggtat aggcatgaat
     3121 ggtcgtcatc gtgccgactt ctattccgaa actgtcgtgc aaggctttgg ccatcggtgc
     3181 aagacagtta gtggtgcatg acgccacgga aacaatggtg tcgttgccat ccagtgtgtc
     3241 gtcattgacg ttataaacga tggttttcat ttcaccggca ggggcggaaa tcaacacctt
     3301 cttcgcacca gcatcaaggt gcgcctgcga tttctcggcg gaggtataaa agccagtaca
     3361 ttcgacaatg atttctgcgc ctttcgcttt ccatggaata tttttagcct ctttttcggc
     3421 gtaaaccgcg atactttttc catcaacgat aagtgaatct tccgtaaaat caacgctcca
     3481 ggggaacggt ccgtagtttg aatcatgttt caacaggtag gcgagaattt ttggggaagt
     3541 gagatcatta atagcaacaa tgtctatgtt gcttttgact tcaagtaatc gacgcaacac
     3601 cagtcgaccg atacgaccaa aaccgttaat accaacttta ctcatggtct tctcctgtca
     3661 ggaacgttcg gatgaaaatt gatcctttcc aggcttagac caggatgtcg ggatgggcaa
     3721 tctacattct tgccgtgaaa cgtaacgtac tgaaaacggg tgaacaatat ttaatgaaat
     3781 tttaagaaaa gcccgttatg ttaacggaaa attatgttaa agcaggaaat gttatggaaa
     3841 ataaatactc aaggttacaa atcagcattc actggttggt ctttttgctg gttatcgtag
     3901 cgtattgcgc aatggagttt cgtggtttct tcccacgtag cgatcggccc ctcatcaaca
     3961 tgattcatgt ttcctgtggc atctcaattc tcgtactgat ggtggttcgt cttttgttaa
     4021 ggctgaaata cccaaccccg ccgattatac ctaagccaaa accgatgatg acgggactgg
     4081 cgcatttggg ccatttggtg atttatctcc tgtttattgc gctgccggtg attggtttgg
     4141 tgatgatgta taaccggggc aacccgtggt ttgcgtttgg tttgacgatg ccttacgctt
     4201 cagaggccaa tttcgagcgg gttgatagct taaagtcgtg gcatgaaacg ctggcgaatc
     4261 tgggatattt tgtcatcggg ttgcacgctg cggctgcact ggcacaccac tatttctgga
     4321 aggacaacac acttctacgc atgatgccac gtaaacgttc ctgaaggata tttaaagaaa
     4381 acgcctgtac taaaaccgac ccgtggtaca ggcgaagaat acgggcctac atcggaagcg
     4441 cctattatat ttatttgtat gacaaataaa accataatcc ttgtctatac gtccatctgg
     4501 cttatcttta atcaattcac ccgatctttg atctcatcaa cggtatcaaa ataaaccagc
     4561 gtataacgtt caaatcgaac ataagattcg ataaatcatg gaggttatat gaaaaaactg
     4621 gcacttatcc tggttatggg aacgcttgtt tccttttatg ccgatgccgg gcgcaaaccc
     4681 tgttctggtt cgaaaggggg gatctcacac tgtacggcag gcggcaaatt tgtctgtaat
     4741 gatggttcta ttagtgcatc gaaaaaaaca tgcactaact gaagtgtaaa aggggtgcca
     4801 tgagaaaatg aatccgctag tcagatgcgc ggggaaaatc cccgcgcttg cccttacctg
     4861 gacgtgcagg ccatgagcgc agcaacctcc ttatcaccgt cccggaaccg cagttcgtag
     4921 agcgtttgtc gggtcaggag tgtgaatgtc agaatcgtaa tgcagataat gagcagacac
     4981 accaccagag ggttgcgctt catagccttt ctccttgccg gattgcgggt aagaggctaa
     5041 gatctgaatt gctgtgttca ttcgttggcc tcggttgata gaaatatcgg tcggggcctt
     5101 cgtctttctg attcccggtt agcctgaaaa cagaaagtct caggcacccg tgagcatcct
     5161 atgtggtttc cttagggacg aaaataatca cctgacgaaa ttgcgtgctg ttttccagaa
     5221 tttttcgtcg ttcggtttag ccagtttaat cattcgttac tctcttcatt ccaatggcat
     5281 tattcttctc tgccataatt gttgtaaaaa tgtgacgcaa agaggttttt ggtcgtaaga
     5341 aattaccgtc aagtgccgat gactttctat caggagtaaa cctggaagag agacaacggt
     5401 aatgaataca actccctcac agcgattagg ttttttgcat cacatcaggc tggttccgtt
     5461 atttgcctac attcttggcg gtatcttagt tctattcgca ttaagttcag ccctggccgg
     5521 ctatttcctt tggcaggccg atcgcgatca gcgtgatgtt actgcggaga ttgagattcg
     5581 gaccgggtta gcgaacagtt cagatttttt gcgttcagcc cggatcaata tgattcaggc
     5641 cggggcagcg agtcgtattg cggaaatgga agcaatgaag cgaaatattg cgcaagccga
     5701 atcggagatt aaacagtcgc agcaagggta tcgtgcttat cagaatcggc cggtgaaaac
     5761 acctgctgat gaagccctcg acactgaatt aaatcaacgc tttcaggctt atatcacggg
     5821 tatgcaacct atgttgaaat atgccaaaaa tggcatgttt gaagcgatta tcaatcatga
     5881 aagtgagcag atccgaccgc tggataatgc ttataccgat attttgaaca aagccgttaa
     5941 gatacgtagc accagagcca accaactggc ggaattggcc catcagcgca cccgcctggg
     6001 tgggatgttc atgattggcg cgtttgtgct tgccctggtg atgacgctga taacatttat
     6061 ggtgctacgt cggatcgtca ttcgtccact gcaacatgcc gcacaacgga ttgaaaaaat
     6121 cgccagtggc gatctgacga tgaatgatga accggcgggt cgtaatgaaa tcggtcgctt
     6181 aagtcgtcat ttacagcaaa tgcagcattc actggggatg acagtaggga ctgttcgaca
     6241 gggtgcggaa gagatttatc gtggcaccag cgaaatttcc gctggcaatg cggacctttc
     6301 atcccgcacc gaagaacaag cggcggcaat cgaacaaact gccgccagca tggagcaact
     6361 cactgcgacg gtgaaacaga atgcggataa cgcgcatcat gccagcaaac tggcgcaaga
     6421 ggcttctatt aaagccagcg atggcgggca gatggtttcc ggtgtagtaa aaacgatggg
     6481 cgctatctcc acgagttcga agaaaatttc tgagatcacc gccgtcatca acagtattgc
     6541 tttccagacg aatattctgg cactgaatgc tgccgttgaa gccgcgcgag cgggtgagca
     6601 aggacgtgga tttgccgttg tcgccagcga agtacggaca ctcgcaagcc gcagcgctca
     6661 ggcggcgaaa gagattgaag gcttgatcag tgaatcagtc aggttaattg acctgggatc
     6721 ggatgaggtg gccacggccg ggaaaaccat gagcactatt gttgatgccg tcgcgagtgt
     6781 cacacatatc atgcaggaaa tcgccgccgc ctcggatgaa caaagcagag gcataacgca
     6841 ggttagccag gcgatttctg aaatggataa ggtgacgcaa cagaatgctt ctctggtaga
     6901 agaggcctca acggcggcgg tgtcccttga agaacaggcg gcacgactaa ctgaggcggt
     6961 ggacgtattc cgtctgaaca aacattctgt gtcggcagaa ccacgaggag cgggtgaacc
     7021 agttagtttt gctacggtgt gaaaatgtta aaggagggat cgacagatcc cttcaccttt
     7081 cagaacggca ttgattttcg aatagcgtta atcatcaact ggcaaccaga agagaacgtc
     7141 gcatctacgc gggtcagtat tccaatcggt tcgcctgcac catgtcccgg aacaggcagg
     7201 gctaccagcg tggcatgacg caggtcgtct tttacagcgc cagaagggac aaaccacacg
     7261 taatcgtatt caaccgtaag ttgacgagat agcgaagcag acagcgtttc gatacaaccc
     7321 gaaggaattt tacatccctg gctctgcact aatgcatctg aatgctggcg tggcgcagtg
     7381 ccttctggtg atacaacgac cggccattcc agcacccggc ttagcgttac gttctcctga
     7441 agtagcggat gattagggcg gacaaccagc ttcagcgatt caagaaacag cagttcgtaa
     7501 ttaagtccgg tcatcagttc aggatctgac atccgaccaa tgccgatatc gatttccccg
     7561 gtttttaaac ccgcaagaat catagggtta ctcattgtcg caacttgcaa ggtcgtctct
     7621 ttttgttgct gatgaaactg acctataacc gaaggtaata tccccagtgc cgcagtcggt
     7681 agtgcaccaa ccctgacgac atcattatta agaccttctt tacgatgaag cgactgtcca
     7741 gcagtgttga tggcgtcaag aactctgact gcatgcgtta aaaattgttc gccgggtaag
     7801 gtaagttgcg ccccctgacg accacgctca aacaagcgag cgcccgtcag ctgctccagt
     7861 tcattcaatg tcttagagag cgcaggttga ctcaaattaa gggtttcagc cgcgcgcccc
     7921 aaagttcctt gttgtgcgac agctacgaat gtatgaaggt ggcgcaaacg gatgcgctga
     7981 ctaaacagac tatttttttc cataagcgat gttaaaaacg aagcggtgtc gctgacaagt
     8041 gaagttgt
//
LOCUS       DADSZD010000176         7902 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.177, whole genome shotgun sequence.
ACCESSION   DADSZD010000176 DADSZD010000000
VERSION     DADSZD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.177"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            14..178
                     /locus_tag="J1Z28_003764"
     CDS             14..178
                     /locus_tag="J1Z28_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718559.1"
                     /translation="MRKFSEQIFKITGDENVAKNELEPWTPEGTDPNYCWWEVDPVDA
                     ANEAMSYYND"
     gene            214..843
                     /locus_tag="J1Z28_003765"
     CDS             214..843
                     /locus_tag="J1Z28_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718560.1"
                     /translation="MAELTKEWLQNMITGIESVIDDKSFVCDEIVFKIGEVKNILTAF
                     KVTLASLEAEPVAEIRAHYPLGINGGKQKFVQAIGDLPDFGGHLYAAPPAPVVPDEGR
                     DQFEALIRFHVGDENHETLLLRANEGMNYRDPNVDFAWIFWKSSREHLLRNNDGSLPE
                     SGHQVRGLTLLVKRLASSLKSVNKSSKLPDKAMDYLKRNGLISVEDVLR"
     gene            complement(1048..1347)
                     /locus_tag="J1Z28_003766"
     CDS             complement(1048..1347)
                     /locus_tag="J1Z28_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7718561.1"
                     /translation="MTFEVIITPEAEQQIINLLRYITEKAGSVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGNKVFIAGIYHGGQSYEADFL"
     gene            complement(1353..1610)
                     /locus_tag="J1Z28_003767"
     CDS             complement(1353..1610)
                     /locus_tag="J1Z28_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HBA7718562.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRTAIRSGK"
     gene            1746..2024
                     /locus_tag="J1Z28_003768"
     CDS             1746..2024
                     /locus_tag="J1Z28_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718563.1"
                     /translation="MAHDTRFYNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFEKALSLDRRQWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            2026..3075
                     /locus_tag="J1Z28_003769"
     CDS             2026..3075
                     /locus_tag="J1Z28_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBA7718564.1"
                     /translation="MRVLLRPVLVPELGLVVVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYSTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPESVLRRSLGLPVEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIVLPHNAHQQQNPPQEKPVVSVTVDPESPESFMKRPKRRRWVNEKYTLWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEEKYGSQVDL
                     IFRFLDHAFATGVLG"
     gene            3088..3447
                     /locus_tag="J1Z28_003770"
     CDS             3088..3447
                     /locus_tag="J1Z28_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBA7718565.1"
                     /translation="MRTEFVLPWPPTVNTYWHRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIKITEVGSA"
     gene            3444..4109
                     /locus_tag="J1Z28_003771"
     CDS             3444..4109
                     /locus_tag="J1Z28_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBA7718566.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGSSIALVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEHQIAITRWAWNEFNAQSGARKIAGKTLVRLKTLIWLASQAVKAE
                     LFGGEGYGYQELALLTGVTTKNWSKTFTGHWVTMKHIFHRLDSETLLLVVRTRSTQKA
                     AFS"
     gene            4363..5076
                     /locus_tag="J1Z28_003772"
     CDS             4363..5076
                     /locus_tag="J1Z28_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718567.1"
                     /translation="MDDSALLRNSSLFVAYMGCLGWGSAYFYGWGTSFYYGFPWWVVG
                     AGVDDVARSLFYAVTVIVIFLIGWGVGIVFFLGIKQKRNIQNLSFIRLFLAILLLFIP
                     PVLEFSVIHQHVEPDVLIFCVIAVFIITLFVRSGRRLVSVSCFSEMYFIRHHRIEFMM
                     AGFMIYFWAFSLIAGWYKPQFKREYQAIHYENVWYYIIARYDDRLVLSKSYSSGGKKF
                     VICNSGNIDDFEINVVRVR"
     gene            5251..5448
                     /locus_tag="J1Z28_003773"
     CDS             5251..5448
                     /locus_tag="J1Z28_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBA7718568.1"
                     /translation="MQKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRHLPQ"
     gene            5599..6657
                     /locus_tag="J1Z28_003774"
     CDS             5599..6657
                     /locus_tag="J1Z28_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBA7718569.1"
                     /translation="MFNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     SWDNQWAGDEDYLKWLDQCLAQFWRVLKPTGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDDGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSTSQWQLPNERDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVAAYTSLNRQYAELQCEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLRQIICASSRPGDMVADFFMGSGSIIKAAMALGRHAIGVELEA
                     ERFEQTAREVLNLISNGH"
     gene            6699..6774
                     /locus_tag="J1Z28_003775"
     tRNA            6699..6774
                     /locus_tag="J1Z28_003775"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6732..6734,aa:Ile,seq:cat)
     gene            6777..6853
                     /locus_tag="J1Z28_003776"
     tRNA            6777..6853
                     /locus_tag="J1Z28_003776"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6811..6813,aa:Lys,seq:ttt)
BASE COUNT         1993 a         1699 c         2108 g         2102 t
ORIGIN      
        1 atataacgag tgggtgagaa aattctctga gcagattttt aaaattactg gcgacgagaa
       61 tgtggcaaaa aatgaattag agccgtggac acctgaagga accgacccaa attattgttg
      121 gtgggaggtt gatccggttg atgctgcaaa tgaagccatg agttactaca acgattaatg
      181 ccggaagact acccgaaaga gaggtaatga aaaatggctg aattaaccaa agaatggcta
      241 cagaacatga ttaccggaat tgagtcggta atagacgata aatcttttgt atgtgatgaa
      301 atagtattca aaatcggtga ggttaaaaac atacttaccg catttaaagt cacgctggca
      361 tcgctggaag cagagccggt ggctgagatt agagcgcact acccattagg gattaacggc
      421 ggtaaacaaa agttcgtcca ggccattgga gatcttcctg actttggcgg acatctgtac
      481 gccgctccgc cagcgcctgt agtgccggat gaggggcgcg accagtttga agcacttatc
      541 aggtttcatg tcggtgatga aaaccatgaa acattattgc tgcgggccaa cgaagggatg
      601 aattaccggg acccaaatgt tgattttgca tggatattct ggaagtccag tcgtgagcat
      661 ctgcttcgga ataatgacgg cagtctgcca gaatcgggtc atcaggtacg cggattaaca
      721 ctgctggtta agcgactggc cagttcatta aaaagcgtca ataaatccag caagctgcct
      781 gataaggcga tggactacct gaagcgaaac ggactgataa gcgtggagga tgttttgcga
      841 tgacctggcc tgaagcattc acaacggtag ggattgcaat ggcggtggcg ctggtggtgt
      901 attcgatttg ccgctgggga taaatcaccg aaaaaagatc ccgacgcata cacgagccgg
      961 gatctttgat ttatatagcc tacgaatccg ccagtaagag agggggcgga cggtttattt
     1021 taacaccgga atgatgtggg taaaagttta taagaaatcg gcttcatagc tttgcccacc
     1081 atgataaata cccgcgataa agactttatt gccatcaacg gcaaaagcaa taatcgttct
     1141 gtggcggaag tgagttaccc gcattccctg gcgaatatca tcgcgtttat tgccccgatg
     1201 cgggaatgta gaaaacccat caagataatc aagaagcgca ttggcataat tgtcagcaat
     1261 gacgcttcct gctttctccg tgatatatct gagcaggttt attatctgct gttcggcctc
     1321 gggggtaatg atgacttcaa atgtcatgca gattacttcc cggatcgaat cgcggtgcga
     1381 acctgtgaaa tggagcgtcc gttgtttggg ttttcgcgga tagagtcaag agagggggcg
     1441 gctgaatgcg ttaaccacgc ttcgattgct ttatcgcgct cattcagtgc gcgaagccct
     1501 tcacgaatga cctcactttc tgaagcatag gcaccggaag ccacacgggc gcgcaccatg
     1561 tcagccatct cgttagttaa tgtaatgctg aattgttggg ttgtacgcat ggtaaacctc
     1621 acggagtagg atagaacact attcgatgat agcacgctgc ctgttgacga caacagaaat
     1681 cagaggcaat attgccgcgc gccagcctga acaactggca cctgctgcgt cagcagagaa
     1741 aactgatggc gcacgatacc agattttaca attcggataa ctctgccgcc cctgccagca
     1801 ggcacgggcg gcgttctcat gcattcaaat ctgactggtt ccagcatgac ccatgcactg
     1861 aagaacaggc cgaatggttg atacagtgct accgcagacg cgggtatgag tttgagaaag
     1921 ccctcagcct tgaccgccgc caatggataa tctctgtaag gctcccttat tccgaacgcc
     1981 caccgcgtcc gtcccgtaca ttccagcaac gcatctggag gtaacgtgcg ggtattactt
     2041 cgacctgttc tggttcctga actcgggctg gtggtcgtta agccgggccg tgagtccatg
     2101 ccggtattcc acaatacccg ggtactggtg gagccggaac cgaaaagcat gcgtaatctg
     2161 ccgtccgggg tcgttcctgc cgttcgccag ccgctggtgg aagacaaaac attgctgccg
     2221 tttttcagta acgcacgggt gattcgtgct gctggtggtg ctggtgcatt gtctgactgg
     2281 ctgttgcgcc atattaaatc ctgccagtgg ccacacggcg attatcatca cagcgaaacc
     2341 gtcattcacc gttatagtac tggcgcgatg gtgttgtgct ggcactgcga caaccagctg
     2401 cgcgaccaga cctccgaatc actcgagcaa cttgctcacc aaaacttgtc agcatggatg
     2461 attgacgtca ttcgtcacgc aatgaatggc acacaggagc gggaattatc gctggctgaa
     2521 ttatcctggt gggcggtctg caatcaggtg gctgatgcgc ttccggagtc tgtattgcgt
     2581 cgttcgctgg gattaccggt ggaaaaaatc cgctccgtat acagtgagag cgacatcata
     2641 ccgggagagc agactgccac cagcattctg aagcagcgca ctaaaaatat tgtgctgccg
     2701 cataacgccc accagcaaca gaacccacca caggaaaagc cggtggtcag cgttaccgtt
     2761 gatccggagt ctccggaatc tttcatgaag cggcctaaac gtcgccgttg ggtaaatgag
     2821 aaatacacgc tctgggtaaa gacacagccg tgtgcatgtt gtggtaagcc agctgacgat
     2881 ccgcatcacc tgattggtca tggtcaggga ggaatgggaa caaaggccca cgatattttc
     2941 acgctaccgc tgtgccggga acatcacaac gaacttcatg cagatccgct ggcgttcgaa
     3001 gaaaagtatg gttcccaggt tgatttgatt tttcgttttc ttgatcacgc ttttgcaact
     3061 ggcgtgcttg ggtgaaagag gtgactgatg cgtacagagt ttgttttgcc ctggccgccg
     3121 acggtgaaca cttactggca tcgtcgtggc agcacatatt ttatatctga ggagggaaag
     3181 cgctatcgcc gggctgtggc gcttattgtt cgccagcagc ggttgaaact aagcctgtcc
     3241 ggaaggctgg cgataaaggt gattgcagag cctccggata agcgacgtcg ggatctggac
     3301 aatatcctga aagcgcccct ggatgcgctg acgcatgcgg ggctgttaat ggacgatgag
     3361 cagtttgatg aaatcaatat tgtacgtggt cagccagtat ctggtggacg cctggagata
     3421 aaaattactg aggtggggag tgcatgaata accagtattt acagtttgtt cgtgagcaac
     3481 tcatgattgc cactgcggat ctcagtggtt cgacaaaagg ccagctggaa gcctggcagg
     3541 agaatgccct gttcgataca gggcgttaca gacgcaaaaa aatccggtac cgcgatgaag
     3601 taaccggaaa aatgataacg cgggataatc caccgatccc gggaaaacaa tcgctggcga
     3661 aaggctcatc aattgccctg gtcagtccgg ttgagttttc aacatcatca tggcggcggg
     3721 cagttctgtc tcttgaagaa catcataaag cctggctgct gtggtgttac agcggtagca
     3781 tttgctggga gcatcagatc gcgataacgc ggtgggcgtg gaatgaattt aatgctcaat
     3841 ccggtgccag aaaaattgca ggaaaaacgc tggtgcgcct gaagacgttg atctggttgg
     3901 cgtcgcaggc ggtaaaagct gagctttttg gtggggaagg ttatggatat caggaactgg
     3961 cgttactgac gggggtaaca accaaaaact ggtccaagac atttactggt cactgggtta
     4021 caatgaaaca catttttcat cggctggata gtgaaacttt attgttagtg gtgagaacgc
     4081 gttcaacaca aaaggcagcg ttttcatagc aaagtattgc aaaagtagat taaaaggcat
     4141 atgttttgtg taaatctgat attctgccga ttttctacgt gatggcaaag ctagtaaaac
     4201 ccgcgaccga gcgggttttt atctcctaaa aaaacagtat cgacattaaa cgttatgacg
     4261 attgtgccaa tactttctcc ttctatgacg ccccttgact gtatggaatc caatttgtta
     4321 tgtaatgcgt gttaatattt ttgagttgtt aatggtgtta ctatggatga cagtgctctg
     4381 ctcagaaact cttcactttt tgttgcttat atgggctgtc tcggatgggg aagcgcttat
     4441 ttctatggat ggggtacttc attttactat ggctttccat ggtgggttgt cggggctggt
     4501 gtcgatgatg tagcacgaag tttgttttat gctgtgacag ttatcgttat attccttatt
     4561 ggatggggcg ttggcattgt tttctttttg ggcataaaac aaaagcgcaa tatacaaaat
     4621 ttgagtttta tcagactttt tcttgcgata ttgctgcttt ttattccacc tgttctggag
     4681 ttttcggtaa ttcatcagca tgttgagcca gatgtactga ttttttgcgt tattgctgtc
     4741 tttataatca cgctttttgt caggtcagga agaagacttg tttcagtcag ttgtttttcg
     4801 gaaatgtatt ttattcgcca tcaccgaatt gagttcatga tggctgggtt tatgatttat
     4861 ttctgggcat tctctcttat tgccggttgg tacaaaccac agtttaagag ggaatatcag
     4921 gcgatccact atgagaatgt ctggtattac attattgcgc gctatgatga tcgtctggtg
     4981 ttatcgaaat catacagtag tgggggtaag aaattcgtta tatgtaatag cggaaatatt
     5041 gatgattttg aaattaatgt tgtcagggtg cgttaaaatt tcctgagtaa caaagatttt
     5101 taccgcccgc cattgagcgg gttttttatg tcagaaaagc ggttcggtac attaaatgtg
     5161 caggtggtta ttaataccgg tctttcagct tgctggcttt ttcgacaaga gctattggtg
     5221 tgtcacgtta accggaaaag ggaaaaagac atgcagaaac agcaggatat gacagaaacc
     5281 gccagagtgg tgtttaatga attgagcgtc accgaaccgg cgacagtcgg ggagattgcg
     5341 cagaacacat acctttcacg cgaacgctgc cagttaatac tgacccagct tgttatggcg
     5401 ggtctggcag actatcagtt tggttgttac agacaccttc cacagtgaag gcttttttat
     5461 ttgtggtaaa tgggcggctg gtgggtgtta ggggcgctca ccagccatct gctcatgcat
     5521 tgggatcaca agcaaacctc aggcccactg ctttgcgcaa aagcagaatg agcctatcag
     5581 agacaggctt aatgatccat gtttaatact gtaaaaatat ccagttgtga gttaatcaac
     5641 gccgactgcc tggaatttat ccggtcgtta cccgaaaatt ctgttgatct gatagtcacg
     5701 gacccgccgt actttaaagt gaaacctgag agctgggata accagtgggc gggtgatgaa
     5761 gattacctga agtggctgga ccagtgtctg gcgcagttct ggcgggtgct gaaacctacc
     5821 ggaagtcttt acctgttctg tggccatcgc ctggcgtctg acatcgaaat catgatgcgt
     5881 gaacgcttta atgtgctgaa ccatatcatc tgggcgaagc catccggacg ctggaacggg
     5941 tgcaacaagg aaagcctgcg tgcgtatttc ccggccacag agcgcattct gtttgcggaa
     6001 cattatcagg gaccgtatcg cccaaaagat gatggctatg aggcgaaggg cagggcactg
     6061 aaacagcatg tgatggcccc gctgatatct tactttcgtg atgcgcgtgc cgctctgggg
     6121 ataacggcaa aacagattgc tgatgccaca ggaaagaaaa acatggtgtc gcactggttc
     6181 agtaccagtc agtggcagtt accgaatgaa cgcgattatc tgaaattaca ggcgttgttt
     6241 gcccgggtgg cagaagaaaa gcatcaacgc ggtgaactgg aaaaacccca ccaccagctg
     6301 gttgccgcgt atacctcact gaaccggcag tatgcggaac tgcagtgtga atataagcat
     6361 ctgcggcggt attttggcgt gacggcgcag gtgccatata ccgatgtgtg gacgcacaag
     6421 ccggtccagt tttacccggg taaacacccc tgtgaaaaac cggcggagat gctgcggcag
     6481 ataatttgtg cgagcagccg tcctggtgac atggtggcgg atttctttat ggggtcgggc
     6541 tcaatcataa aagccgctat ggcgcttggg cgtcatgcaa ttggtgttga actggaagct
     6601 gaacgttttg agcaaacggc cagggaagtt ctgaatttaa tcagtaatgg tcactgatat
     6661 tgcagaatta aatccccgtg attattattc tgcattccgg ccctttagct cagtggtgag
     6721 agcgagcggc tcataaccgc caggtcgctg gttcaaatcc agcaagggcc accatgccgt
     6781 cactagctca tcaggaaaga gcatcaacct tttatgttga gagtgcgggg ttcgagtccc
     6841 cggtgacggt ccagtaaaaa aatgatgagc tggctttcag gaccgttttt aattcacagc
     6901 aaaaagagcc cgtggcgtgt ttatcgtttt tcccgaactg taacagaaaa cattgatagt
     6961 tcctgtgccg tgttttataa cgctgaatgg ggtggggcat tgttggtggg agttctggta
     7021 cttcctgaca gggactgacg atgcgccacc ccgacgttgt taaaattacg aaaaaatcct
     7081 gttgtctgtt tgaatgccgc cttaactggc ggttttttgc aactttatac cggactgtat
     7141 atcaggtaag cattcagtta tcgtgccata taacaaaatc cgaaaatgct atgcctgcat
     7201 attgttagtt ggcttatgtt tttgtatgtt gctgcaccgc aggattctcc cctgcaacga
     7261 aggttgtatg atgaataatt ttttatcttt cctttttcca gtcatcctca gatgactggc
     7321 tcctttttga gcttgtcgat aatacggggg atgccacttc aggggataaa gttattgcgt
     7381 tgccggggaa gcattgtcag ctttttttaa gataactcct gtgagttatg tgcataaatg
     7441 ctacaaactc cacgttttat atggttggga gttgccgggc gcgcagttag tttgctaaga
     7501 aaacgcctgc atggtgaatc cccctgtgcg gtggggtgat accattaatt tcctgtgttg
     7561 ccgactggta tcacggacgg tctgttcacc gggaggcacc cggcaccatg catttgaatt
     7621 tcttttttta aagatttgat tcggaatatt cctgtaacca gaattaagaa ttgataaata
     7681 actcacattt tatggtgctt cgtattgtca gagggacgtg atgttttgta tttttttagc
     7741 gcatggtgaa tccccctgtg cggaggggca taatgactgt taggcttggt cgcattagcg
     7801 ttagtagcaa gttgcggatt ctgtctggtc attgcagaat tcaccgggag gcacccggca
     7861 ccgtgcaatt caatactttt gttgttcttg tttaatctgc tg
//
LOCUS       DADSZD010000177         7847 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.178, whole genome shotgun sequence.
ACCESSION   DADSZD010000177 DADSZD010000000
VERSION     DADSZD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.178"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            133..222
                     /gene="kdpF"
                     /locus_tag="J1Z28_003777"
     CDS             133..222
                     /gene="kdpF"
                     /locus_tag="J1Z28_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBA7718570.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            222..1895
                     /gene="kdpA"
                     /locus_tag="J1Z28_003778"
     CDS             222..1895
                     /gene="kdpA"
                     /locus_tag="J1Z28_003778"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBA7718571.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLAPVALLIALFFIQQGALQNFLPYQAVTTIEGSQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICTGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1918..3966
                     /gene="kdpB"
                     /locus_tag="J1Z28_003779"
     CDS             1918..3966
                     /gene="kdpB"
                     /locus_tag="J1Z28_003779"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBA7718572.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSITVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3975..4547
                     /gene="kdpC"
                     /locus_tag="J1Z28_003780"
     CDS             3975..4547
                     /gene="kdpC"
                     /locus_tag="J1Z28_003780"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBA7718573.1"
                     /translation="MRGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4540..7224
                     /gene="kdbD"
                     /locus_tag="J1Z28_003781"
     CDS             4540..7224
                     /gene="kdbD"
                     /locus_tag="J1Z28_003781"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdbD"
                     /protein_id="HBA7718574.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRTAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7221..>7847
                     /gene="kdpE"
                     /locus_tag="J1Z28_003782"
     CDS             7221..>7847
                     /gene="kdpE"
                     /locus_tag="J1Z28_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBA7718575.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPAR"
BASE COUNT         1622 a         2073 c         2320 g         1832 t
ORIGIN      
        1 ctaaacatta gttaaatcat ggcttttgcc atttttatac tttttttaca ccccgcccgc
       61 agatttttgc gaaatctttg cagccagaac tctacccttc cggtatcact tttaggccac
      121 tggaggtgca ctgtgagtgc aggcgtgata gccggcgtgt tgctggtgtt tttattactg
      181 ggttatctgg tttatgccct gatcaatgcg gaggcgttct gatggctgcg caagggttct
      241 tactgattgc cacgttttta ctggtattaa tggtgctggc gcgtccctta ggtagcgggc
      301 tggcgcggct gattaatgat atacctcttc ctggtacgac gggcgttgaa cgcgtactct
      361 ttagcgcgct tggcgtctct gaccgcgaga tgaactggaa gcagtatctc tcagccattc
      421 ttggcctgaa tatactgggg ctggcggtgc tgttttttat gttgctcggt cagcactatc
      481 tgccgcttaa tccacagcag ttgccggggc tgtcgtggga tctggcgctc aataccgccg
      541 tcagctttgt cacgaacacc aactggcaat cttatagcgg tgaaaccacg ttgagctatt
      601 tcagccagat ggcgggctta acggtgcaaa actttctttc tgccgccagc gggattgcgg
      661 tgatttttgc gcttattcgc gcgtttaccc gccagagcat gagcacgctc ggtaatgcct
      721 gggtcgatct gctacgcatc acgttatggg tgttagcccc tgtggcattg ttgatcgcac
      781 tgttttttat acaacaaggt gcgctgcaaa actttttgcc ttatcaggct gtgacgacca
      841 ttgaaggatc gcaacagctg ttaccaatgg ggccagtagc ttctcaggaa gcgatcaaaa
      901 tgctcggaac taacggcggt ggctttttta atgccaactc gtcgcatccg tttgaaaacc
      961 caaccgcact gaccaacttc gtgcaaatgc tggcgatttt cttgatccca acagcgctgt
     1021 gctttgcctt tggtgaagtg gcgggcgatc gtcgccaggg gcgcatgttg ctgtgggcga
     1081 tgtcggtgat ttttgtcatc tgcacaggcg tagtgatgtg ggcagaagtt cagggtaatc
     1141 cgcatctgct ggcgctcggc gcggacagca gcatcaatat ggaaggtaaa gagagccgtt
     1201 tcggcgtgct ggtcagtagc ctgtttgcgg tcgtgactac ggcggcttcc tgcggcgcgg
     1261 tgattgcgat gcatgattcg tttaccgctc ttggtggcat ggtgccgatg tggttgatgc
     1321 aaattggtga agtggtattc ggcggcgtcg gttccggcct ttacggcatg atgctgtttg
     1381 tcctgctggc ggtgtttatt gccgggctga tgattggtcg tacaccggaa tatctgggta
     1441 aaaaaatcga cgtacgcgag atgaaactga ctgcactggc gattctggtc accccgacgc
     1501 tggtgctgat gggcgcggcg ctggcaatga tgaccgacgc cggacgtagc gccatgctca
     1561 accctggccc gcatggtttt agcgaagtgc tgtacgccgt gtcatccgcc gctaacaaca
     1621 acggcagcgc ctttgccgga ttaagcgcca actctccatt ctggaactgt ttactggcgt
     1681 tgtgcatgtt tgtcgggcgc ttcggggtga ttatcccggt gatggcaatt gccggttcgc
     1741 tggtgagtaa aaagagccaa cccgccagct ccggcacatt gccaacgcac ggcccgctgt
     1801 ttgttggcct gttaatcggc accgtgttgc tggttggcgc actgaccttt atccctgccc
     1861 tggcgcttgg tccggtggcg gaatatctct cctgatgata ttgagtgagc actgaatatg
     1921 agtcgtaaac aactggcgct attcgaacca acacttgtcg ttcaggcgct gaaagaagcg
     1981 gtgaaaaaat taaacccgca ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc
     2041 ggaagtctgc tgaccacctg tattagcatc gcgatggcaa gcggtgtgat gcctggcaat
     2101 gcgctgttta gcgcggcaat tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc
     2161 gccgaggcgt tggcagaagg ccgcagtaaa gcgcaggcca acagtctgaa aggggtgaaa
     2221 aaaaccgctt ttgctcgcaa actgcgcgaa ccgaaatatg gcgctgcggc ggacaaagtt
     2281 cctgctgatc aactgcgtaa aggtgacatc gtgctggtag aagctggcga tattatcccc
     2341 tgcgatggtg aagtcattga aggcggcgca tcggtcgatg aaagcgccat caccggggaa
     2401 tcagcaccgg tgatccgtga atccggcggt gattttgcct ccgtcactgg cggcacgcgt
     2461 attctttctg actggctggt gattgagtgc agcgttaacc ccggcgagac atttctggat
     2521 cggatgatcg cgatggtgga aggtgcgcag cgacgtaaaa cgccgaacga gattgccctg
     2581 accattctcc tgattgccct gactatcgtc tttttactgg caaccgccac gctgtggccg
     2641 ttttccgcgt ggggcggtaa tgcagtcagc ataacggtac tggtggcact gctggtctgt
     2701 ctgatcccaa ccactattgg cggcctgttg tcagcgattg gcgtcgccgg gatgagtcgg
     2761 atgctgggcg cgaatgttat tgccaccagc gggcgagcag ttgaagcagc gggtgatgtt
     2821 gacgttctgc tgctggataa aaccggcacc atcaccctcg gtaaccgtca ggcgtcagag
     2881 tttatccccg cacaaggcgt ggaggaaaaa gcgctggccg acgccgcaca actggcttcg
     2941 ctggctgatg aaacgccgga aggccgcagt attgtgatcc tcgccaagca gcgttttaac
     3001 ctgcgcgagc gcgatgtgca gtcgctccat gccacctttg taccgtttac tgcacaaagc
     3061 cggatgagcg ggatcaacat cgacaaccgc atgatccgta aaggttctgt cgatgccatt
     3121 cgccgccatg ttgaggctaa cggcggtcac ttccctgccg atgttgatca aaaagtcgat
     3181 caggttgcgc gtcagggagc cacgccgctg gtggtggtgg aaggttctcg tgtgctgggc
     3241 gttattgcgc tgaaagatat cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgt
     3301 aaaatgggca ttaaaacggt gatgattacc ggcgataacc gcctgactgc cgccgcgata
     3361 gctgcggaag cgggtgtcga tgattttctc gccgaagcaa caccggaggc caagctggca
     3421 ctgattcgtc agtatcaggc ggaaggccgt ttagtggcga tgaccggcga tggcaccaac
     3481 gatgctccgg cgctggcaca ggccgatgtt gcggtggcga tgaactccgg cacccaggcg
     3541 gcgaaagaag cgggcaatat ggtggatctc gactccaacc cgaccaagtt gatcgaggta
     3601 gtgcacattg gcaaacagat gctgatgacc cgtggctcgt tgaccacctt cagcattgcc
     3661 aacgatgtgg cgaaatactt cgccattatt ccagcggcgt tcgcggcaac gtatccgcag
     3721 ttaaatgcgc tgaacatcat gcgcctgcat tcgcccgact ccgccattct cagtgcggtc
     3781 attttcaacg ccttgattat cgtctttttg attcccctgg cgttaaaagg cgtgagttat
     3841 aaaccgctta ccgcttctgc catgttgcgc cgtaacttat ggatttacgg tctgggtggg
     3901 ctgctggtgc cgtttattgg tatcaaagtc attgatttac tgctaaccgt ttgcggtctg
     3961 gtgtgaggtt taccatgcgt ggattacgtc cggcattatc gacatttctc tttctgttat
     4021 tgattactgg cggcgtttac ccgctgctga ccaccgcact ggggcaatgg tggtttccct
     4081 ggcaggccaa cggttcattg attcgtgaag gtgatacggt gcgcggttcg gcattaatcg
     4141 ggcaaaattt taccggcaac ggctattttc atggtcgccc gtcggcaaca gcagaaatgc
     4201 cctataatcc acaggcttct ggcgggagca atctggcagt cagtaacccg gagctggata
     4261 aacagatagc cgcacgcgtt gccgcattac gggctgctaa cccggatgcc agcacgaacg
     4321 ttccggttga actggtgacg gcgtcagcaa gcgggctgga caataacatc accccgcaag
     4381 cggcggcctg gcagatccca cgcgtggcga aagcgcgtaa tctcagcgtt gaacagctca
     4441 cgcaactgat cgcaaaatac agccaacaac cgctggtgaa atatatcggc cagccggtcg
     4501 tcaacattgt cgaactcaat ctggcactgg ataagcttga tgaataacga acccttacgt
     4561 cccgaccccg atcgtctgct ggaacaagct gccgcgccgc atcgggggaa gctgaaagta
     4621 ttcttcggtg cctgcgcagg cgtcgggaag acttgggcga tgctggcgga agcccagcga
     4681 ctgcgggcgc aagggctgga tattgtggtt ggcgtggtag aaacccacgg tcggaaagat
     4741 accgccgcca tgctgaaagg tctggctgtt ctgccgttaa aacgccaggc gtaccgtggg
     4801 cggcacatca gcgagtttga tctcgatgcc gccctcgccc gccgtccggc actgatctta
     4861 atggacgaac tggcgcacag taatgcgcca ggttcacgtc atcctaaacg ctggcaggac
     4921 atcgaagagc tgctggaagc tggcattgat gttttcacta ccgtcaacgt tcagcatctg
     4981 gaaagtctga atgatgtggt cagcggcgtc accggaattc aggtacggga gaccgtgccc
     5041 gatccttttt ttgatgccgc cgacgacgta gtactggtgg acttgccccc ggacgatctg
     5101 cgccagcgac tgaaagaagg caaagtctat attgccgggc aggcggagcg cgccattgaa
     5161 cattttttcc gcaaaggtaa tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc
     5221 gatcgcgtag atgagcaaat gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg
     5281 cacacacgcg acgcgatcct tttatgcatc gggcataaca ccggcagtga gaaactggtc
     5341 cgcacggcgg cgcggttggc ctcgcgcctg ggcagtgtct ggcacgcggt gtatgttgaa
     5401 acccctgccc tgcatcgctt accggagaaa aaacgtcggg caattctcag cgccttacgt
     5461 ctggcgcagg agctgggcgc ggagacggca acactttctg atccagcgga agagaaagcg
     5521 gtagtgcgtt atgcccgtga acataatctc ggcaagatta ttctcggtcg cccggcttcg
     5581 cgccgctggt ggcgtcggga aacatttgct gaccggctgg cacgcatcgc ccccgatctc
     5641 gatcaggtgc tggtcgcgct tgatgaacca cccgcccgca cgattaacaa cgcgccggat
     5701 agccgctctt ttaaagacaa gtggcgggta cagattcagg gatgcgtggt tgccgccgcg
     5761 ttatgcgccg ttatcacctt aattgccatg cagtggctga tggcgtttga tgccgccaac
     5821 ctggtgatgc tgtatctgct tggcgtcgtg gtggtggcac tattttacgg acgctggcct
     5881 tcggtggttg ccaccgtcat taatgtggtg agtttcgatc tcttttttat cgccccacgc
     5941 ggcacgctcg ccgtctctga tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc
     6001 gggctggtga tcgggaacct tactgctggc gtgcgttatc aggcgcgggt agcccgttac
     6061 cgcgaacaac gcacacggca cttatatgaa atgtccaaag ctctggctgt gggccgtagt
     6121 tcgcaggata tcgccgccac cagcgaacag tttattgcct ccacgtttca tgcccgtagc
     6181 caggtgttgc tgcctgatga caacggtaaa ttgcagcctt taacacatcc gcaaggaatg
     6241 acgccgtggg acgatgccat cgcgcagtgg agttttgata aaggcctgcc tgcgggcgcg
     6301 ggcaccgaca cgttacccgg tgtaccgtac cagattttgc cgctaaaaag cggcgagaaa
     6361 acctacggac tggtggtggt ggaaccgggg aatctgcgcc agttgatgat cccggaacag
     6421 cagcgcctgc tggagacgtt tacgctgtta gtcgccaatg cccttgagcg actgacacta
     6481 accgccagcg aagaacaggc gcggatggca agcgaacgtg aacagatccg caacgccctg
     6541 ctggcggcgc tttcgcatga tttacgcacg ccgcttacgg tgctgttcgg tcaggcagaa
     6601 atcttaacgc tcgatctggc aagcgaagga tcgccccacg cccgccaggc cagcgagatt
     6661 cgtcagcatg tgctgaacac cacccgactg gtgaataatc tactggatat ggcgcgaatt
     6721 cagtccggcg gctttaattt gaagaaagag tggttaacgc tggaagaagt ggtcggcagc
     6781 gcgctgcaaa tgctggaacc gggtttatcg tcgcccatca atctttctct gccagagccg
     6841 ctgaccttaa tccacgttga cgggccactc tttgaacggg tgctgattaa cctgctagag
     6901 aacgcggtga aatatgcggg ggcgcaggcc gaaattggta tcaatgccca cgttgagggc
     6961 gaaaatctac aactggatgt ctgggataac ggccccggtc ttccaccagg ccaggagcag
     7021 acgatatttg ataagtttgc tcgcgggaat aaagagtcgg cggtaccggg tgtagggctt
     7081 gggttggcaa tttgtcgggc gatagtggat gtacacggcg gcaccattac cgcattcaac
     7141 cgaccggaag gtggtgcctg ttttcgtgtt acacttcccc agcaaacagc ccctgaactt
     7201 gaagaatttc atgaggatat gtgacaaacg ttctgattgt tgaagatgaa caggctattc
     7261 gtcgctttct gcgcacggcg ctggagggcg acggtatgcg cgtctttgag gccgaaacgc
     7321 tgcaacgcgg cttgctggaa gcggcaaccc gtaagccaga tttgattatt ctcgatctcg
     7381 gcctgcccga cggtgatggg attgagttta tccgcgacct gcgccagtgg agcgcggtgc
     7441 cggtgattgt gctttccgca cgcagcgaag agagcgataa aatcgccgcg ctggatgccg
     7501 gagcggatga ttatctgagt aagccgtttg gcattggcga attgcaggcc cgtctgcgcg
     7561 tcgcattacg ccgccactcc gccaccacca cgcccgatcc gctggtgaaa ttttctgatg
     7621 ttaccgtcga tttagccgcc cgcgttatcc accgcggcga ggaagaggtg catctcacac
     7681 cgattgagtt ccgcctgctg gccgtgctgc tcaacaacgc cggaaaagtg ctcacccagc
     7741 gccagttact caatcaggtg tggggaccaa acgcggtcga acacagccac tatttgcgta
     7801 tttatatggg gcatctgcga caaaaactgg agcaagatcc cgcccgc
//
LOCUS       DADSZD010000178         7843 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.179, whole genome shotgun sequence.
ACCESSION   DADSZD010000178 DADSZD010000000
VERSION     DADSZD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.179"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            63..914
                     /locus_tag="J1Z28_003783"
     CDS             63..914
                     /locus_tag="J1Z28_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBA7718576.1"
                     /translation="MLKYSKTPGIFKLEGNRLGRPYHRLPTLFTGNFDVIDSHLGSYF
                     LKKHRSNITLKKIGCEMDIINKNAELMVSQVGHLAFDIDRSLLLMLLGNFYGLESSLE
                     EAKSHNGLPTKTETRLKNRLALDICTLIFNLQTSGIALKLKLDSSTVITHWAYQLTFT
                     LAGDEESSFRILLDDAHTDFILNLIRHSEHSKPQQVAKSVNKPALIKEIIRSMPLTLN
                     VKIAELSLNVADLAQIKAGDILPISLGETFPVAIGQSELFSALIVEDKDKLFLSELAG
                     KNENNHE"
     gene            907..1278
                     /locus_tag="J1Z28_003784"
     CDS             907..1278
                     /locus_tag="J1Z28_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718577.1"
                     /translation="MSKQEDILAGDFGLTDEVAPVAKSADAETLVTRLEDRFSDSMTL
                     LKRIPVTLTLEVSSVEIMLADLLNIDDDTVIELDKLAGEPLDIKVNNILLGKAEVVVV
                     NEKYGLRVLEFNTRDINDLAP"
     gene            1275..2027
                     /gene="fliP"
                     /locus_tag="J1Z28_003785"
     CDS             1275..2027
                     /gene="fliP"
                     /locus_tag="J1Z28_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016149597.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBA7718578.1"
                     /translation="MKRRTQLTLGLALLALAPLALAQGGDIALLNVVTHGNTQEYSVK
                     IQVLILMTLVGLLPTMVLMMTCFTRFIIVLSLLRQALGLQQTPPNRILIGIALSLTLL
                     VMRPIWLNIYDHAVVPFENDQITLTDALSTAATPLKRFMLAQTDKKAMAQIMTIGGAK
                     GNAADQDLSIVVPAYVLSELKTAFQIGFMIYIPFLVIDLIVASVLMAMGMMMLSPLIV
                     SLPFKLMLFVLIDGWSLTIGTLTTSIRGLGLG"
     gene            2030..2302
                     /gene="fliQ"
                     /locus_tag="J1Z28_003786"
     CDS             2030..2302
                     /gene="fliQ"
                     /locus_tag="J1Z28_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003838859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliQ"
                     /protein_id="HBA7718579.1"
                     /translation="MLTVDVAADIVASGIKVVILLVSVLVVPSLLVGLLVSVFQAVTQ
                     INEQTLSFLPRLIVTLVVLGMCGKWMIIQLHDLCIHLFTQAALLVH"
     gene            2304..3086
                     /gene="fliR"
                     /locus_tag="J1Z28_003787"
     CDS             2304..3086
                     /gene="fliR"
                     /locus_tag="J1Z28_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HBA7718580.1"
                     /translation="MRTSDVTQLMDLALGLWFPFVRIMAFLRYMPVLDNSALTARVRI
                     ILSLALAIIITPLIPHPVPHDLLSLNSLILTVEQILWGMLFGLMFQFLFLALQLAGQI
                     LSFNMGMSMAVMNDPSSGTSTTVLAELINVYAILLFFAMDGHLLLVSVLYKGFTYWPI
                     GNALHPQTLRTIALAFSWVLASASLLALPTTFIMLIVQGCFGLLNRIAPPLNLFSLGF
                     PINMLAGLVCFATLLYNLPDHYLHLANFVLQQLDALKGHYGG"
     gene            3076..4215
                     /gene="flhB"
                     /locus_tag="J1Z28_003788"
     CDS             3076..4215
                     /gene="flhB"
                     /locus_tag="J1Z28_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBA7718581.1"
                     /translation="MADSSSEEKTEKPSAQKLRKAREEGQLPRSKDMGLAASLFAAFV
                     VISSSFPWYADFVRESFISVHQYAQEINNPQVIGQFLRHHLLILGKFILTLLPMPAAA
                     LLSSLVPGGWLFLPKKILPDFSKISPLKGIGRLFSSEHLAETGKMTVKSVVVLVMLWV
                     SLRNNFAAFLGLQALPFKLAMNEGLSLYASVMRNFVILFIFFALLDVPLAKALFTKGL
                     KMTKQELKEEYKNQEGKPEVKARVRRLQRQLAMGQIRKVVPKANVVITNPTHYAVALQ
                     YDSSRAAAPFVVAKGTDEIALYIRQVATENQVEVVEFPRLARSVYYTTQVNQQIPFQL
                     YRAIAHVLTYVLQMKHWREGAQPRPALNRHISIPKEVLKLDGENN"
     gene            4199..6292
                     /gene="flhA"
                     /locus_tag="J1Z28_003789"
     CDS             4199..6292
                     /gene="flhA"
                     /locus_tag="J1Z28_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhA"
                     /protein_id="HBA7718582.1"
                     /translation="MAKTTKSFLALLRGGNLGVPLVILCILAMVILPLPPALLDILFT
                     FNIVLAVMVLLVAVSAKRPLEFSLFPTILLITTLMRLTLNVASTRVVLLHGHLGAGAA
                     GKVIESFGQVVIGGNFVVGFVVFIILMIINFIVVTKGAERISEVSARFTLDAMPGKQM
                     AIDADLNAGLINQTQAQTRRKDVASEADFYGAMDGASKFVRGDAIAGMMILAINLIGG
                     VCIGIFKYNLSADAAFQQYVLMTIGDGLVAQIPSLLLSTAAAIIVTRVSDNGDIAHDV
                     RNQLLASPSVLYTATGIMFVLAVVPGMPHFPFLMFSALLGFTGWRMSKRPQAAEAEEK
                     SLETLTRTMTETSEQQVSWETIPLIEPISLSLAYKLVALVDKAQGNPLTQRIRGVRQV
                     ISDGNGVLLPEIRIRENFRLKPSQYAIFINGIKADEADIPADKLMALPSSETYGEIDG
                     VLGNDPAYGMPVTWIQPAQKAKALNMGYQVIDSASVIATHVNKIVRSYIPDLFNYDDI
                     TQLHNRLSSMAPRLAEDLSAALNYSQLLKVYRTLLTEGVSLRDIVTIATVLVASSAVT
                     KDHILLAADVRLVLRRSITHPFVRKQELTVYTLNNELENLLTNVVNQAQQAGKVMLDS
                     VPVDPNMLNQFQITMPQVKEQMKAAGKDPVLLVPPQLRPLLARYARLFAPGLHVLSYN
                     EVPDELELKIMGALS"
     gene            6397..6675
                     /locus_tag="J1Z28_003790"
     CDS             6397..6675
                     /locus_tag="J1Z28_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBA7718583.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            6668..7024
                     /locus_tag="J1Z28_003791"
     CDS             6668..7024
                     /locus_tag="J1Z28_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBA7718584.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIIDVLEREFSAQM"
     gene            complement(7081..7830)
                     /locus_tag="J1Z28_003792"
     CDS             complement(7081..7830)
                     /locus_tag="J1Z28_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBA7718585.1"
                     /translation="MPLPSIPSFVLSGLLLLCLPFSSFASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LAENNHFLSSDHLHNITEVAIYRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         1770 a         2046 c         2175 g         1852 t
ORIGIN      
        1 gtgtcaccgc cggatttgcg tgcacaaaat cgcccatacc attacagcag agaaagtaag
       61 ccatgctgaa gtacagtaaa acgccaggca tctttaaatt agaaggtaat cggcttggac
      121 gcccctacca tcgcctgccg accctgttta cggggaattt tgacgtaatt gattcgcatc
      181 ttggcagcta ctttctcaaa aagcatcgca gcaatatcac cctaaaaaag attggctgcg
      241 aaatggatat tattaataaa aatgccgagc tgatggtttc ccaggtcggg catctggcgt
      301 ttgatattga tcgctcctta ttgttaatgc tgctgggcaa tttttatggt ctggaatcgt
      361 ctcttgaaga agcgaaatca cacaatggat taccgaccaa aacggaaaca cgtttaaaaa
      421 accgcctcgc gctggatatc tgcacgctga tttttaatct gcaaacctcc ggtattgcgc
      481 tgaaattaaa actggacagc agcacggtaa ttacccactg ggcgtatcaa ttaaccttta
      541 cccttgcggg cgatgaagag agcagttttc gtattttgct cgacgacgcc cacaccgatt
      601 ttattttgaa tctgatccgc cacagcgagc acagtaaacc gcagcaggtg gcgaaatccg
      661 ttaataaacc ggccctgatt aaagaaatca tccgctctat gccgctgacc ctgaacgtca
      721 aaatcgccga gttgtcgctg aatgtcgcgg atctggcgca gataaaagcg ggggatattt
      781 tacccatctc gctgggagaa acgttcccgg tggcgattgg gcaatctgaa ttatttagcg
      841 ccctgattgt ggaagacaaa gacaaactgt ttttgtcgga gctggcggga aaaaatgaga
      901 acaaccatga gtaagcaaga agatatttta gccggggatt ttggcctcac cgatgaagtt
      961 gcgcctgtcg ccaaatccgc cgacgccgaa acgctggtca cccgcctgga agaccgattt
     1021 tccgactcga tgacgctgct gaaacgtatt ccagtgacct taacgctgga agtatcgtcg
     1081 gtggagatta tgttagccga cctgcttaat atcgacgacg acacggtgat tgaactggat
     1141 aaactcgccg gggagccgct ggatattaaa gtgaacaata tcctgctggg caaagcggaa
     1201 gtggtggtgg tcaacgagaa gtacggcctg cgcgtgctgg aatttaacac ccgcgatatc
     1261 aacgacctgg caccatgaaa cgccgtactc aactgacgct gggcctggcg ctgctggcgc
     1321 tcgccccgct ggcgttggcc cagggtggcg atattgcgct gcttaacgtg gtgacccacg
     1381 gcaacactca ggagtacagc gtcaaaattc aggtactgat tctgatgacc ctcgtcggtt
     1441 tgctgccgac gatggtgctg atgatgacct gctttacgcg ctttattatc gtgctgtcgc
     1501 tgctgcgcca ggcgctgggg ctgcaacaaa cgccgccgaa ccgcattctg attggtattg
     1561 cgctctcact caccctgctg gtaatgcgcc ccatctggct caatatttac gaccacgcgg
     1621 tagtgccgtt tgaaaacgat cagattaccc tgaccgatgc gttaagcacc gccgcgacgc
     1681 cgctgaaacg ctttatgctg gcgcagaccg acaaaaaagc gatggcgcaa atcatgacca
     1741 tcggcggcgc gaagggcaat gccgccgacc aggatctctc catcgtggtg cccgcctatg
     1801 tactgagcga gctgaaaacc gccttccaga ttggctttat gatttacatc ccgtttctgg
     1861 ttatcgacct gattgtcgcc agcgtattga tggcgatggg gatgatgatg ctgtcgccgc
     1921 tgattgtttc gctacccttt aaactgatgc tgtttgtgct gattgacggc tggtcgctga
     1981 ccatcggcac gctcacaacc agcattcgcg ggctggggct gggctgatta tgttgaccgt
     2041 agacgtagcc gccgacattg tcgccagcgg cattaaagta gtgattttgc tggtttcggt
     2101 actggtggtg ccaagcctgc tggtcggcct gctggtgagc gtgttccagg cggtgacgca
     2161 gattaacgaa cagaccctga gctttttgcc aaggctgatt gtcacgctgg tggtgctggg
     2221 catgtgcggc aaatggatga ttatccagtt gcatgatctc tgcattcacc tgtttaccca
     2281 ggccgcgttg ctggttcatt gagatgcgca caagcgacgt tacgcaactg atggatctgg
     2341 cgctggggct gtggtttccc ttcgtgcgca ttatggcgtt tctgcgctat atgccggtgc
     2401 tggataacag cgccctgacc gcgcgcgtgc ggattatttt gtcgctggcg ctggcgatca
     2461 tcatcacgcc gttgatcccc catcctgttc ctcacgacct gctttcgctc aatagcctga
     2521 tcctgacggt agagcaaatt ttatggggca tgttattcgg gctgatgttt cagtttttgt
     2581 ttctggcgct tcaactcgcc gggcaaattc tctcgttcaa tatggggatg agtatggcgg
     2641 tgatgaacga cccgagcagc ggcacatcga ccaccgtgct ggcggagcta atcaacgtct
     2701 acgcgatcct gctgtttttt gccatggacg ggcatctgct gctggtaagc gtgttgtaca
     2761 agggcttcac ctactggccg attggcaacg ccctgcatcc gcaaaccctg cgcaccatcg
     2821 cgctggcctt tagctgggtg ctggcgtcgg catcgctgct ggcgctgccg accactttta
     2881 tcatgctgat tgtgcagggc tgctttggcc tgctcaatcg cattgcgccg ccgctgaacc
     2941 ttttttcgct cggctttccc atcaacatgc tggcggggct ggtctgcttt gccaccctgc
     3001 tctacaacct gccggatcac tatctgcatc tggcgaattt tgtcttacaa caactcgacg
     3061 cgctgaaagg tcactatggc ggatagcagc agcgaagaaa aaacagagaa accctcggcg
     3121 caaaagctgc gtaaagcgcg tgaagagggg cagctaccgc gctcgaagga tatggggctg
     3181 gcggcgagcc tgtttgcagc gtttgtggtg atctccagca gttttccgtg gtatgccgat
     3241 tttgtgcgcg agagttttat cagcgtgcat cagtacgcac aggagattaa taacccgcag
     3301 gtgatcggtc agtttctgcg ccaccacctg ttaattctcg gcaagtttat ccttacgttg
     3361 ctgccgatgc ccgccgccgc gctgctctct tcgctggtgc cgggcggctg gctgtttctg
     3421 ccgaaaaaaa tcctcccgga tttcagcaaa atcagcccgt taaaaggcat tgggcgactg
     3481 ttttccagcg aacatctggc ggaaacgggg aagatgacag tgaagtcggt ggtggtgctg
     3541 gtaatgctgt gggtaagcct gcgcaataac tttgccgcct ttctcggcct gcaggcgctg
     3601 ccgttcaaac tggcgatgaa tgaggggtta tcgctctacg ccagcgtgat gcgcaacttt
     3661 gtcatcctgt ttatcttctt tgcgcttctc gacgtgccgc tggcgaaagc cctgttcacc
     3721 aaagggctga agatgaccaa acaggagctg aaagaagagt acaaaaatca ggaaggcaag
     3781 ccggaagtga aggcgcgcgt gcggcggctg caacggcaac tggcgatggg gcaaattcgc
     3841 aaagtggtgc cgaaagccaa cgtggtgatc accaacccga cccactacgc cgtggcgtta
     3901 cagtatgact catcacgtgc cgccgcgccg tttgtggtgg cgaaaggcac cgatgaaatc
     3961 gccctctata tccgccaggt cgccactgaa aatcaggtgg aagtggtcga gttcccgcga
     4021 ctggcgcgct cggtttacta caccacccag gtgaaccagc aaattccgtt tcagctttac
     4081 cgggctatcg cccacgttct gacctacgtc ctgcaaatga aacactggcg cgagggcgca
     4141 cagccgcgcc cggccctgaa cagacatatt tccattccca aagaggtact taaactggat
     4201 ggcgaaaaca actaaatcgt tcctcgcgct gctgcgcggc ggcaatctcg gcgtgccgct
     4261 ggtgatcctg tgtattctgg cgatggttat tctgccgctg ccgcctgcgc tgctggatat
     4321 tctgttcacc ttcaacattg tgctggcggt gatggtgctg ctggtggcgg tgtcggcgaa
     4381 acgaccgctg gaattcagcc tgttcccgac cattttgctg atcaccaccc taatgcgcct
     4441 gacgcttaac gttgcctcta cgcgcgtggt gctgctgcac gggcatctcg gcgcgggcgc
     4501 ggcgggtaag gtgattgagt cgtttggtca ggtggtgatt ggcggcaact ttgtcgtcgg
     4561 tttcgtggta tttatcatcc tgatgatcat caactttatc gtcgtcacca agggggccga
     4621 gcgtatttcc gaggtttctg cccgctttac cctggacgcg atgcccggca aacagatggc
     4681 gattgacgcc gatcttaacg ccgggttgat caaccagacg caggcgcaaa cccggcgtaa
     4741 agatgttgcc agcgaggccg atttctacgg cgcaatggac ggggcatcaa aatttgtgcg
     4801 cggggatgcc atcgccggga tgatgattct ggcgatcaac ctgatcggcg gcgtctgtat
     4861 cgggatcttc aaatacaacc tgagcgccga cgccgccttc cagcaatatg tgctgatgac
     4921 tatcggcgat gggctggtgg cgcaaatccc ttccctgctg ctctccaccg cggcggcgat
     4981 tatcgtcacc cgcgtcagcg ataacggtga tatcgcccat gacgtgcgta atcagttgct
     5041 ggcaagcccg tcggttctct acaccgccac tggaattatg ttcgtgctgg cggtggtgcc
     5101 gggaatgccg catttcccgt tcctgatgtt cagcgccctg cttggtttta ccggctggcg
     5161 gatgagcaag cgcccgcaag cggcggaggc ggaagagaaa agcctcgaaa cgctaacccg
     5221 tacgatgaca gaaaccagcg agcaacaggt cagttgggaa actattccgc tgatcgaacc
     5281 tatcagttta agcctcgcct acaaactggt ggcgctggtg gataaagcgc agggcaaccc
     5341 gctcacccag cgtattcgcg gcgtacgtca ggtgatttcc gacggcaacg gcgtgctgct
     5401 gccggagatc cgtattcggg aaaacttccg cctcaagccc agccagtacg ctattttcat
     5461 caacggcatt aaggctgatg aagcggatat tccggcggat aaactgatgg ccctgccctc
     5521 cagcgaaacc tacggcgaga tagacggcgt gctggggaac gacccggcgt acgggatgcc
     5581 ggtcacctgg attcagcccg cgcagaaagc gaaggcgctg aatatggggt atcaggtgat
     5641 cgacagcgcc agcgtaattg ccacccatgt gaataagatt gtgcgcagct atattcctga
     5701 tttgtttaac tatgacgata ttactcagct gcataaccgt ttgtcgtcga tggcaccgcg
     5761 actggcggaa gatttgagcg cggcgctcaa ttacagccag ttgctgaaag tgtaccgcac
     5821 actactgact gaaggcgttt ccctgcgcga tatcgtcacc atcgccaccg tgttggtcgc
     5881 cagtagtgcg gtgactaaag atcatattct gctggcggcc gatgtgcgcc tggtgctgcg
     5941 gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg acggtgtata cgctgaataa
     6001 tgagctggaa aatctgctga ccaacgtggt gaatcaggcg caacaggccg gcaaagtgat
     6061 gctcgatagc gtgccggtgg acccgaatat gctcaaccag tttcagatca cgatgccgca
     6121 ggtgaaggag cagatgaaag cggcggggaa agatccggtg ctgctggtgc cgccgcagct
     6181 gcgccctttg ctggcgcgct acgcaaggtt gtttgcacca gggctgcatg tgctgtcgta
     6241 taacgaagtg ccggatgagc tggagttgaa gataatgggg gcgttgagtt gattgaggaa
     6301 tcttttccat gctttgcagc acctgacacc attaattgac gtcacactgc aaagagacta
     6361 cgatgacacc atacaatgac gtcatggatt gatagtatga ttaaggtcag cgcgctaggc
     6421 tctcgtcata gaaaaacatt cgacgatgta atgacggagc cgccaaaatc tggaattaaa
     6481 tgggatgatg ttgttgcact cattaaagct gtgggtggaa caataaaaaa taacaacggc
     6541 tccagacgaa aatttcagat tggaacgacc aaattcagca cacatgagcc acatccaaag
     6601 aatgtgatgg ataaaggcgc agtagcaggt cttaaggagt ggtttgttaa ctgtgtaggg
     6661 gtagattatg aataaagcat cgaacattat cacaattaaa ggtcaaccag ccgcagtgac
     6721 atttgaagcc gatatcaacg catttcgcgg caagttcctc aatgtaaacg gttattgtga
     6781 ctttgtagcc acaagtattg aggcattata tcgtgaaggc gaaagcgcgc tggatgagtg
     6841 gatggcggat tgtgaagagg acggtattgc cccttttcgt gaagaagaag agcaaaaacg
     6901 cctgacgctt cgtgttcctc atcacattga ttcgcggctg accatcgtcg ccagacaaca
     6961 ttccatttca aaaaaccagc tcattattga tgtcttagaa cgtgagtttt cagcccagat
     7021 gtaaacaggt cggatgccgc gtggcatccg accttttgcc agttcgtcaa tattacccat
     7081 ttacaaaatc gtctcttccg tcaaaatcct gcgcgcgccc aaaaagtggt cctgccagaa
     7141 aggttcggct aaccggctta cccgtatggt ttcccccgtg cgtggcgatt cgataaattg
     7201 cccgtctccc agatacacgc ccatatgatc ggcaatctca cggctgtgaa tatggaaaaa
     7261 cagcaaatct ccccggcgca ggtcgttatt cgccacaatc gttgcccgat ggtagtgata
     7321 catctcattg gccgtacgcg ggagcttcgc ctcgaggatc ttgttgtagg cataaaaaac
     7381 caacccgcta cagtcgaagc cttgatcggg ccgcgtgccg ccccagacgt aaggcttacc
     7441 aagctgctgc tccaggcggt aaatcgccac ttcggtgatg ttgtgtagat ggtcgctgct
     7501 taagaaatgg ttgttctccg ccagcgcctg ccagcgtctg tcactcgcct tgagcggcgc
     7561 aggaaaccat tctggatgct gttttatctg ttcgcggttg tgctggcgca gcgcccttct
     7621 gctttcggcg ttgccttcca caatgtagct ggcctgcttt ttcagatggg tttggtactg
     7681 ttttaataaa cgcgcacggt tttgcatccg ctggcgggcg gcgtagctga aagagatatg
     7741 tgatgtggtg gcgctggcaa atgaagaaaa cggcaagcaa agcaacaata atcccgacaa
     7801 cacaaaagag ggaatcgacg gcaaaggcat tctcaataat ggc
//
LOCUS       DADSZD010000179         7832 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.180, whole genome shotgun sequence.
ACCESSION   DADSZD010000179 DADSZD010000000
VERSION     DADSZD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.180"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            32..1198
                     /locus_tag="J1Z28_003793"
     CDS             32..1198
                     /locus_tag="J1Z28_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBA7718586.1"
                     /translation="MIEHYIRGFEEELREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFNGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQLGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGSCMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            1241..2557
                     /locus_tag="J1Z28_003794"
     CDS             1241..2557
                     /locus_tag="J1Z28_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718587.1"
                     /translation="MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            2661..3311
                     /gene="fabR"
                     /locus_tag="J1Z28_003795"
     CDS             2661..3311
                     /gene="fabR"
                     /locus_tag="J1Z28_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBA7718588.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            3311..3670
                     /locus_tag="J1Z28_003796"
     CDS             3311..3670
                     /locus_tag="J1Z28_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBA7718589.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(3710..4810)
                     /gene="trmA"
                     /locus_tag="J1Z28_003797"
     CDS             complement(3710..4810)
                     /gene="trmA"
                     /locus_tag="J1Z28_003797"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBA7718590.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      4924..5114
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5179..7017
                     /gene="btuB"
                     /locus_tag="J1Z28_003798"
     CDS             5179..7017
                     /gene="btuB"
                     /locus_tag="J1Z28_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBA7718591.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFQQPINTVLA
                     PTDIVTREDIERWQSRTVLDVMRRLPGVDVAQYGGMGQSSSLFIRGTESKHVLVLVDG
                     IPVARPGISNSSDIDQIPVSLVQRIEYIRGPRSAVYGSGAIGGVVNIITMTNKERSQI
                     NSSIGSKGYQQYDGTYRQRFGDTVVTAAGAYQTTKGFNVQPHSSYSGDGDRDGYRNKL
                     FWGGIEHKFNDQFDGYFRGYGYASNADYDQGNWGYDGGNDEHQNYTQSWDTGLRFNSG
                     VYSSQLVVNYQRIKDYNYSSLTGRYASGYTLDDMEQRYIQWGNNVIVGHGAISAGADW
                     KQEKLMSFGANGTDNYKRNNTGLYLTGQQQIDNITLEASGREDHDGQLGWHGTWQTAA
                     GWQFVDGYKVTLSYGTGFLAPSLGQQFGAERFGIASNPNLKPEESKQWEAGLEGLTGP
                     VDWRLSTYRYKIQNLIDYNNNTYFNVKSATIKGLEWTGNFTTGPIDHRLTLQYVDPRD
                     DETDQQLYRRAKQQVKYELSGEVYELGWDVTYQYIGERYDYDYDSTRKVKMGGLSVWD
                     IGLSYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            6962..7819
                     /gene="murI"
                     /locus_tag="J1Z28_003799"
     CDS             6962..7819
                     /gene="murI"
                     /locus_tag="J1Z28_003799"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBA7718592.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         1802 a         1825 c         2138 g         2067 t
ORIGIN      
        1 gcctgtttat aagtgaataa aggaatagcc gatgatcgaa cactatattc gtgggtttga
       61 ggaagaactt cgcgagatcc gccatcagat ccacgaaaac ccggagctgg gattacaaga
      121 atttaaaacc agtgctttgg ttgcggaaaa gctgcgccag tggggctatg aggttgagca
      181 agggctggcg acaaccggcg tggtagccac gttaaaagtg ggcgacggag agaaaagtat
      241 tggcctgcgg gcggatatgg atgcgttgcc gatttatgaa aatagtggca agccctgggc
      301 cagtaaacat cctggtttaa tgcacgcctg cgggcatgac gggcatacca ccatactgct
      361 gggggctgcg cgttattttg ccgaaacccg ccgttttaac ggcacgttac gccttatctt
      421 ccagcctgcg gaagagatga ttaacggcgg tgagatcatg gttaaagagg ggctttttga
      481 tcgtttcccc tgcgatgtca ttttcgggat gcacaacatg ccgggtttgc cggtcggcaa
      541 gtttttcttc cagcttgggg cgctgatggc gtcaatggat cagttccata ttacggttcg
      601 tggttgtggc ggacacggtg cgatcccgca caaagccatc gatccagtgc tggttgccgc
      661 acatatcacc accgcattac aaagcattgt gtcgcgcaat gtcgatccgc tggaagcggc
      721 ggtaattacc gtcggcagca ttgttgcggg tgaggcggct aacgtcattc ctgacagtgc
      781 tgagatgaaa atcagtgtcc gctctcttag ccgcgatacc cgacaacttc ttctgacccg
      841 tattcctgct cttgcacaag cccaggctgc cagctttggc gctacggctg aggttacgca
      901 tgttaacggt acaccggtat tagtaaatga cgaggagatg gcgcgcttcg cctggcaggt
      961 ggcgtgcaaa acatttggtg aagatcgggc tgagtttggc atcaaacccc tgatgggcag
     1021 tgaagatttc tctttcatgc tggaagccca acccaaaggc ggtttcctgt tattcggtaa
     1081 tggcgatgtc ggggaaggtt cctgcatggt gcataacccc ggttacgact ttaacgatgc
     1141 aagcctggtc ccggcaagca gctactgggg cgcactggtt gaagcctggc tgcaataaat
     1201 aaaacaacac acaacaacac tttttggcaa ggaacatgga atgacggcga tcgtaaatga
     1261 aaccgtgagt gtgaagaaaa agcccagcgg caggcgggtg atctttgcct cggcgtttgg
     1321 caatgcgctc gaattttttg attttggcgt ttacaacttt tttgtggtct atatcagtac
     1381 gcttttcttc ccacccagtg ccgatcataa cgtcgcgctg ttactggcat ttgcaacgtt
     1441 tggtgtgagc ttttttatgc gtccgctcgg tgggattatc gttggtgcct gggccgatcg
     1501 cttcgggcgt aaaccggcga tggtgttcac tattgctctg atgagcctcg gtacgctgat
     1561 gatcggcatc gcaccgacgt atgaaacggc gggctattgg ggaactgcaa cgctggtgct
     1621 ggcgcgtcta attcagggcg ttgcggcagg tggtgaagtc ggggcttcaa tgtcgctact
     1681 ggtggagtca gctccggcaa atcgtcgcgg cttttacagc agttggtcac tggcgaccca
     1741 agggctggca acgaccttcg gtggggttgt tgctttaggt ctgagcgcat ggctaccgtt
     1801 cgccacaggt tcagaaaccg taatggcaga atggggctgg cgtgtgccgt tttttattgg
     1861 cgtgttatta gcacctatcg gttgctggtt gcgtctgagc ctggaaaatg acgtacctga
     1921 gccagtgcgc aataaaaaag ctgctaccag cgaaagcgcg ttttctctgc tcctgcaaca
     1981 taaggcgact atcgttaatg gcgttttact cgctattggt agcacggtcg caacctatat
     2041 ctccctcttt tattacggca cctgggcagc gaagtatctg gcgatgcctc aacattattc
     2101 tcatgcagcg atgttactgg cgggggtaat tacgtttgtg ggtgcgctgt tggtggggat
     2161 gctatgtgat tcggttgggc gtaaaaagct gattttaatc tcccgtgtga tggtgcttct
     2221 ctgtagctgg ccgtcattct ggctgttggt gaattaccca agccctggca tgttgctgac
     2281 ggtggttttc gtgatggtca gctttaccac gcttggcggt gtgccagtga tgttgttgat
     2341 ctctgaactg ttgccgaaac ggattcgggc attaggtttc gcgctggttt atagcattgg
     2401 cgtagcaatc tttggcggtt ttgcgcaata tttcgccacg caatccatcg tgttgctgga
     2461 tagtctgacg gctccggcgt ggtatctggg tggaggcacg ttgctgtcga tgctggcgtt
     2521 gttgtacgta aaagaaccgg ccaaagaatt gcagtaaata tgttttattg cgttaccgtt
     2581 cattcacaac actggagcaa tccagtatgt tcattctctg gtatagtgcc agcagtactt
     2641 ttggcaagga ttcagacatc gtgatgggcg taagagcgca acaaaaagaa aaaacccgcc
     2701 gttcgctggt ggaagccgca tttagccaat taagtgctga acgcagcttc gccagcctga
     2761 gtttgcgtga agtggcacga gaagcaggca ttgctcccac ctctttttat cggcatttcc
     2821 gcgacgtaga cgaactgggt ctgaccatgg ttgatgagag cggtttaatg ctacgccaac
     2881 tcatgcgtca ggcgcgtcag cgtatcgcca aaggcgggag tgtgatccgc acctcggtct
     2941 ccacatttat ggagttcatc ggtaataatc ctaatgcctt ccggttatta ttgcgggaac
     3001 gctccggcac ctccgctgcg tttcgtgccg ccgttgcgcg tgaaattcag cacttcattg
     3061 cggaacttgc ggactatctg gaactcgaaa accatatgcc gcgtgcgttt actgaagcgc
     3121 aagccgaagc aatggtgacg attgtcttca gtgcgggggc cgaggcgttg gatgtcggcg
     3181 tcgagcaacg tcggcaatta gaagagcgac tggtactgca gctgcgaatg atttcgaaag
     3241 gggcttatta ctggtatcgc cgtgaacaag agaaaaccgc aattattccg ggaaatgtga
     3301 aggacgagta atgaaacaag caaatcaaga tagaggtacg ctgctgctgg cgctagtggc
     3361 tggcttatcg attaatggta ctttcgcagc gctgtttagc tccattgtac cattttctgt
     3421 attcccgatt atttccctgg tgttgacagt ttactgcctg catcaacgtt atcttaatcg
     3481 cactatgccg gtaggcttgc cgggtctggc agctgcttgt tttattctcg gcgtactgct
     3541 gtacagcacg gtagttcgtg cggaatatcc ggatatcggc tctaacttct tcccggcagt
     3601 actgtccgtc attatggtgt tctggattgg cgcgaagatg cgtaaccgta agcaggaagt
     3661 tgctgagtaa tcggtatcat gccggatgca agcgcatccg gcatgggttt tacttcgcgg
     3721 tcagtaatac gccgcactcc atatggtgcg tgtaggggaa ctgatcaaat agagccagac
     3781 gttcgacctt gtgcgtctgg cttaatgttt ccagattctt gcataacgtt tccggattgc
     3841 aggagatata cagaatacgt ggatacgcct gcaccatttt ctcggtttca ctgtccagac
     3901 cgctgcgcgg tgggtcgaca aaaatggttt cgcactgata actctttaag tcgattcctt
     3961 gcaggcggtt aaactcgcgc acaccattca tcgcctgagt aaattcttct gccgccatac
     4021 gaataatttg tacgttatca atatggttag cggcaatgtt gtattgcgcg gcggcgactg
     4081 acggcttggc gatttcggtg gctaataccc gatcaaaatt gcgtgccagc gctaatgaaa
     4141 agttaccgtt gccgcagtat agctccagta aatcgccttt tgagcctttg gttacgtcca
     4201 gcgcccattc cagcatctga atattcatcg ccgcgttcgg ttgggtaaag ctgttttcta
     4261 cctgacggta gatcatctct ttccccgcga tcggcagacg ttcgtcgatg taatcctgat
     4321 ccagcgcgat tttggttttc gttgcccgac caatcagatg cacattcaga ttctgcgcgc
     4381 gcagtgcatc gcgcagggct tccgcctgct gacgccactc atcatccagc tttttatggt
     4441 acagcaagga aacaaccgct tgattgctta gcgtagtgag gtaatcaatc tggaacaact
     4501 tgtggcgcag aatgggatta ttacgcacac ccgcaatcat cgccgtcatc aactggttga
     4561 taagttcact ggcggcgggg aagctatcca cacggatacg acttttggtt tgttgatcga
     4621 aaatgatgtg gtacaggtca tcgccatcgt gccagatgcg gaactccgca cgcatccgat
     4681 aatgactgac cggcgagcga aacacttccg gaaccaggtc agaaaacggt gccatcatac
     4741 tttgcaaacg taccactttt tcggctaact gcgcttcata ctgttctgtt ggaaggtgtt
     4801 cgggggtcat gatgtatcct gaaaaattaa agtacgcggc gattgtaggg attgctcatc
     4861 agatgtccag atcttgatga attcctattt gtgagctacg tctggacagt aacttgttac
     4921 aacctgtagc atccacttgc cggtcctgtg agttaatagg gaatccagtg cgaatctgga
     4981 gctgacgcgc agcggtaagg aaaggtgcga tgattgcgtt atgcggacac tgccatccgg
     5041 tgggaagtca tcatctctta gtatcttaga tacccctcca agcccgaaga cctgccggcc
     5101 aacgtcgcat ctggttctca tcatcgcgta atattgatga aacctgcggc atccttcttc
     5161 tattgtggat gctttacaat gattaaaaaa gcttcgctgc tgacggcgtg ttccgtcacg
     5221 gcattttccg cttgggcaca ggataccagc ccggatactc tcgtcgttac tgctaaccgt
     5281 tttcaacagc ctatcaatac tgttctggct ccgacggata ttgtcacgcg tgaggatatc
     5341 gaacgctggc aatccagaac cgttctggat gtgatgcgcc gcttacctgg tgttgatgtc
     5401 gcacagtatg gcggcatggg acagagttct tctcttttta tccggggaac cgaatcgaaa
     5461 cacgttctgg tgttagttga tggaattcca gtagcacgcc ctggtatatc gaatagctca
     5521 gacattgacc agatcccagt ctcgctggtc cagcgtattg aatatattcg tggcccacgc
     5581 tcggctgttt atggctctgg tgcaattggt ggtgttgtca acatcatcac tatgacgaat
     5641 aaagagcgtt cgcaaattaa ctccagtatc ggttcgaaag gctaccaaca gtacgatggc
     5701 acataccgcc agcgttttgg agatacggtg gtgactgctg caggggctta tcagacgacc
     5761 aaaggtttca atgttcagcc acactcaagt tatagcggag atggcgatcg cgatggttat
     5821 cgtaacaagc tgttctgggg gggaattgag cacaaattta acgatcaatt tgatggttac
     5881 ttccgtggct acggttatgc cagtaatgcc gattacgacc aagggaactg gggatatgat
     5941 ggcggcaatg atgagcatca aaattacaca cagtcatggg atactggatt gcgttttaat
     6001 tcgggagtct actcttcgca actggtggta aattatcagc gcattaagga ctacaactac
     6061 agcagtctga ctgggcgtta cgcatcaggt tatacacttg atgatatgga gcaacgttac
     6121 atccagtggg gtaacaatgt cattgttggg catggtgcta tcagcgcagg agcagactgg
     6181 aaacaggaaa aactgatgtc ttttggcgct aatggcacgg ataactacaa gcgtaataac
     6241 actgggcttt atctaaccgg gcagcagcaa attgataaca tcacactaga agcctctggt
     6301 cgtgaggatc acgatggcca gctaggttgg catggcactt ggcaaacggc agcagggtgg
     6361 cagtttgttg atggttacaa agtaacgctt tcctatggaa ctggtttcct tgctccttcg
     6421 cttggtcaac aatttggcgc agagcggttt ggtattgctt ctaaccctaa cttgaaacca
     6481 gaagaatcta agcaatggga ggcaggatta gaaggcttga caggtcctgt tgactggcga
     6541 ttgtcgacct atcgctacaa aattcagaac ctgatcgact acaacaacaa tacctatttt
     6601 aacgttaaat ccgcgaccat taagggcctg gaatggacgg gtaatttcac aactggtcct
     6661 atcgatcatc gtttaacgtt gcagtatgta gacccccgtg atgatgaaac tgatcaacaa
     6721 ctctatcgcc gtgcaaaaca gcaggtaaaa tatgagttga gcggtgaggt ttacgagttg
     6781 gggtgggatg tcacgtatca gtacattggt gaacgatacg attacgacta tgacagtacc
     6841 cgtaaggtga aaatgggggg actgagcgtg tgggatatcg gactttcata tccggtcacc
     6901 tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta cgagacagtc
     6961 tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac cttctgaacc
     7021 acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct atgacgagat
     7081 ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg ctttcccgta
     7141 tggcgaaaaa agcgaagtgt ttattgttga gcgagtggtg gaaattgtca ccgcagtgca
     7201 agaacgttat ccccttgcgc tggctgtggt cgcttgcaac accgccagta ccgtttcact
     7261 tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc
     7321 tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg gaactgttaa
     7381 acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct
     7441 gggctcggca gagatggttg agttggctga agcgaagctg catggcgaag atgtttctct
     7501 ggatgcgcta aaacgtatct tgcgtccgtg gttaagaatg aaagagccgc cagataccgt
     7561 tgtactgggg tgtacccatt tccctctact acaagaagaa ctgttacagg tgctgccaga
     7621 gggaacccgg ctggtggatt ctggcgcagc gattgctcgc cgaacggcct ggctgttaga
     7681 acatgaagcc ccggatgcaa aatctgccga tgcgaatatt gccttttgta tggcaatgac
     7741 gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa
     7801 actggcagtt ttaggctgat ttggttgaat gt
//
LOCUS       DADSZD010000180         7746 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.181, whole genome shotgun sequence.
ACCESSION   DADSZD010000180 DADSZD010000000
VERSION     DADSZD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.181"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            293..3094
                     /gene="sucA"
                     /locus_tag="J1Z28_003800"
     CDS             293..3094
                     /gene="sucA"
                     /locus_tag="J1Z28_003800"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBA7718593.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQEKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            3109..4326
                     /gene="odhB"
                     /locus_tag="J1Z28_003801"
     CDS             3109..4326
                     /gene="odhB"
                     /locus_tag="J1Z28_003801"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBA7718594.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPTLAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            4420..5586
                     /gene="sucC"
                     /locus_tag="J1Z28_003802"
     CDS             4420..5586
                     /gene="sucC"
                     /locus_tag="J1Z28_003802"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBA7718595.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            5586..6455
                     /gene="sucD"
                     /locus_tag="J1Z28_003803"
     CDS             5586..6455
                     /gene="sucD"
                     /locus_tag="J1Z28_003803"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBA7718596.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            6695..>7746
                     /locus_tag="J1Z28_003804"
     CDS             6695..>7746
                     /locus_tag="J1Z28_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBA7718597.1"
                     /translation="MGVNSDVYAADVNIDILSATVKDKRIEGVSVTLQRNGAQSVSGT
                     TNASGSVNLGSTFADDQDALLIVKKEGYSNLVVKCSCAGMTYAISPAMTSLDGMRVVL
                     SWGEKPFDLDSHLIFPGGHIYFDSKEGTDANLDVDDTDSYGSETVTISKKHFGESYIY
                     AVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIWTVFKLNPNGEFE
                     DINSVTSANFNDTTLGVRDLATVIMPATGSSTPASPAMQNSGDTQLARKYNREGEAVY
                     KTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWANRKAISLASGANA
                     ATTRANSYYNIAKIYET"
BASE COUNT         1950 a         1976 c         2116 g         1704 t
ORIGIN      
        1 atccggccta caaaagaaat gcagaaaccg taggcctgat aagacgcaca agcgtcgcat
       61 caggcaaaca gcgccggatg cggcgtgaat gccttatccg gcctacaaaa gaaatgcagg
      121 aaatctttaa aaactgcccc tgacactaag acagttttta aaggttcctt agcggacgca
      181 gaaaacacga caagtccgca acaagtgaac cccggcacgc acatcagata tgaaatgtgc
      241 gtggtagtat ccacggcgaa gtaagcataa aaaagatgct taagggatca cgatgcagaa
      301 cagcgctttg aaagcctggt tggactcttc ttacctctct ggcgcaaacc agagctggat
      361 agaacagctc tatgaagact tcttaaccga tcctgactcg gttgacgcta actggcgttc
      421 gacgttccag cagttacctg gtacgggagt caaaccggat caattccact ctcaaacgcg
      481 tgaatatttc cgccgcctgg cgaaagacgc ttcacgttac tcttcaacga tctccgaccc
      541 tgacaccaat gtgaagcagg ttaaagtcct gcagctcatt aacgcatacc gcttccgtgg
      601 tcaccagcat gcgaatctcg atccgctggg actgtggcag caagagaaag tagccgatct
      661 ggatccgtct ttccacgatc tgaccgaagc agacttccag gagaccttca acgtcggttc
      721 atttgccagc ggcaaagaaa ccatgaaact cggcgagctg ctggaagccc tcaagcaaac
      781 ctactgcggc ccgattggtg ccgagtatat gcacattacc agcaccgaag aaaaacgctg
      841 gatccaacag cgtatcgagt ctggtcgcgc gactttcaat agcgaagaga aaaaacgctt
      901 cttaagcgaa ctgaccgccg ctgaaggcct tgaacgttac cttggcgcaa aattccctgg
      961 cgcaaaacgc ttctcgctgg aaggtggtga cgcgttaatc cctatgctta aagagatgat
     1021 ccgccacgct ggcaacagcg gcacccgtga agtggttctc gggatggcgc accgtggtcg
     1081 tctgaacgtt ctggtgaacg tgctgggtaa aaaaccgcaa gacttgttcg acgagtttgc
     1141 cggtaaacat aaagaacacc tcggcacggg cgacgtgaaa taccacatgg gcttctcgtc
     1201 tgacttccag accgatggcg gcctggttca cctggcgctg gcgtttaacc cgtctcacct
     1261 tgagattgta agcccggtcg ttatcggttc tgttcgtgcc cgtctggaca gacttgatga
     1321 gccgagcagc aacaaagtgc tgccaatcac cattcatggt gacgccgcag tgaccgggca
     1381 gggcgtagtt caggaaaccc tgaacatgtc gaaagcgcgt ggttatgaag ttggcggtac
     1441 ggtacgtatc gttatcaaca accaggttgg cttcaccacc tctaacccgc tggatgcccg
     1501 ttcgacgccg tactgtactg atatcggtaa gatggttcag gcaccgattt tccacgttaa
     1561 cgcggatgat ccggaagccg ttgcctttgt tacccgtctg gcgctcgatt tccgtaacac
     1621 ctttaaacgt gatgtcttca tcgacctggt atgctaccgc cgtcacggcc acaacgaagc
     1681 cgacgagccg agcgcaaccc agccgctgat gtatcagaaa atcaaaaaac atccgacgcc
     1741 gcgcaaaatc tacgctgaca agctggagca ggaaaaagtc gcgacgctgg aagatgccac
     1801 cgagatggtt aacctgtacc gcgatgcgct ggatgctggc gattgcgttg tggcagagtg
     1861 gcgtccgatg aacatgcact ctttcacctg gtcgccgtac ctcaaccatg aatgggacga
     1921 agagtacccg aacaaagttg agatgaagcg cctgcaggaa ctggctaaac gcatcagcac
     1981 ggtgccggaa gcggttgaaa tgcagtctcg cgttgccaag atttatggcg atcgccaggc
     2041 gatggctgcc ggtgagaaac tgttcgactg gggcggcgcg gaaaacctcg cttacgccac
     2101 gctggttgac gaaggcattc cggttcgcct gtcgggtgaa gactccggtc gcggtacctt
     2161 cttccaccgc cacgcggtga tccacaacca gtctaacggt tccacttaca cgccgctgca
     2221 acatatccat aacggccagg gcgcgttccg cgtctgggac tctgtactgt ctgaagaagc
     2281 cgtactggcg tttgaatacg gttatgccac cgcagaacca cgtaccctga ccatctggga
     2341 agcacagttc ggtgacttcg ccaacggtgc acaggtggtt atcgaccagt tcatctcctc
     2401 tggcgaacag aaatggggcc ggatgtgtgg cctggtgatg ttgctgccgc acggttacga
     2461 agggcagggg ccggagcact cctccgcgcg tctggaacgt tatctgcaac tttgcgctga
     2521 gcaaaacatg caggtttgcg taccttctac cccggcacag gtttaccaca tgctgcgtcg
     2581 tcaggcgttg cgcgggatgc gtcgtccact ggtcgtgatg tcgccgaaat ccctgctgcg
     2641 tcatccgctg gcggtatcca gcctcgaaga actggcgaac ggcaccttcc tgccagccat
     2701 cggtgaaatc gacgagcttg atccgaaggg cgtgaagcgc gtagtgatgt gttctggtaa
     2761 ggtttattac gacctgctgg aacaacgtcg taagaacaat caacacgatg tcgccattgt
     2821 gcgtatcgag caactctacc cgttcccgca taaagcgatg caggaagtgt tgcagcagtt
     2881 tgctcacgtc aaggattttg tctggtgcca ggaagagccg ctcaaccagg gcgcatggta
     2941 ctgcagccag catcatttcc gtgaagtgat tccgtttggg gcttctctgc gttatgcagg
     3001 ccgcccagcc tccgcctctc cggcggtagg gtatatgtcc gttcaccaga aacagcaaca
     3061 agatctggtt aatgacgcgc tgaacgtcga ataaataaag gatacacaat gagtagcgta
     3121 gatattctgg tccctgacct gcctgaatcc gtagccgatg ccaccgtcgc aacctggcat
     3181 aaaaaacccg gcgacgcagt cgtacgtgat gaagtgctgg tagaaatcga aactgacaaa
     3241 gtggtactgg aagtaccggc atcagcagac ggcattctgg atgcggttct ggaagatgaa
     3301 ggtacaacgg taacgtctcg tcagatcctt ggtcgcctgc gtgaaggcaa cagcgcgggt
     3361 aaagaaacca gcgcgaaatc tgaagagaaa gcgtccactc cggcgcaacg ccagcaggcg
     3421 tctctggaag agcaaaacaa cgatgcgtta agcccggcga tccgtcgcct gctggctgaa
     3481 cataacctcg acgccagcgc cattaaaggc accggtgtgg gtggtcgtct gacccgtgaa
     3541 gatgtggaaa aacatctagc gaaagccccg gcgaaagagt ctgctccggc agcggctgct
     3601 ccggcggcgc aaccgaccct ggctgcacgt agcgaaaaac gtgtgccgat gactcgcctg
     3661 cgtaagcgtg tggcagagcg tctgctggaa gcgaaaaact ccaccgccat gctgaccacg
     3721 ttcaacgaag tcaacatgaa gccgattatg gatctgcgta agcagtacgg tgaagcgttt
     3781 gaaaaacgcc acggcattcg tctgggcttt atgtccttct acgtgaaagc ggtggttgaa
     3841 gccctgaaac gttacccgga agtgaatgcg tctatcgacg gcgatgatgt ggtctaccac
     3901 aactatttcg acgtcagtat ggcggtttct acgccgcgcg gcctggtgac gccggttctg
     3961 cgtgatgtcg ataccctcgg catggcagac atcgagaaga aaatcaaaga gctggctgtt
     4021 aaaggccgtg acggcaagct gacggttgaa gatctgaccg gtggtaactt caccatcacc
     4081 aacggtggtg tgttcggttc cctgatgtca acgcctatca tcaacccacc gcagagcgca
     4141 attctgggta tgcacgctat caaagatcgt ccgatggcag tgaatggcca ggttgagatc
     4201 ctgccgatga tgtacctggc gctgtcctac gatcaccgtt tgatcgatgg tcgcgaatcc
     4261 gtgggcttcc tggtaacgat caaagagttg ctggaagatc cgacgcgtct gctgctggac
     4321 gtgtagtagt ttaagtttca cctgcactgt agaccgacgt ggatagggtg tcggtctacg
     4381 gtttaaaaga taacgattac tgaaggatgg acagaacaca tgaacttaca tgaatatcag
     4441 gcaaaacaac tttttgcccg ctatggctta ccagcaccgg tgggttatgc ctgtactact
     4501 ccgcgcgaag cagaagaagc cgcttcaaaa atcggtgccg gtccgtgggt agtgaaatgt
     4561 caggttcacg ctggtggccg cggtaaagcg ggcggtgtga aagtggtaaa cagcaaagaa
     4621 gacatccgtg cttttgcaga aaactggctg ggcaagcgtc tggtaacgta tcaaacagat
     4681 gccaatggcc aaccggttaa ccagattctg gttgaagcag cgaccgatat cgctaaagag
     4741 ctgtatctcg gtgccgttgt agaccgtagt tcacgtcgcg tggtctttat ggcctccacc
     4801 gaaggcggcg tggaaatcga aaaagtggcg gaagaaactc cgcacctgat ccacaaagtt
     4861 gcgcttgatc cgctgactgg cccgatgccg tatcagggac gcgagctggc gttcaaactg
     4921 ggtctggaag gtaaactggt tcagcagttc accaaaatct tcatgggcct ggcgaccatt
     4981 ttcctggagc gcgacctggc gctgatcgaa atcaacccgc tggtcatcac caaacagggc
     5041 gatctgattt gcctcgacgg caaactgggc gctgatggca acgcactgtt ccgccagcct
     5101 gatctgcgcg aaatgcgtga ccagtcgcag gaagatccgc gtgaagcaca ggctgcacag
     5161 tgggaactga actacgttgc gctggacggt aacatcggtt gtatggttaa cggcgcaggc
     5221 ctggcgatgg gcaccatgga catcgttaaa ctgcacggcg gcgaaccagc taacttcctt
     5281 gacgttggcg gcggcgcaac caaagaacgt gtaaccgaag cgttcaaaat catcctctct
     5341 gacgacaaag tgaaagccgt tctggttaac atcttcggcg gtatcgttcg ttgcgacctg
     5401 atcgctgacg gtatcatcgg cgcggtagcg gaagtgggtg ttaacgtacc ggtcgtggta
     5461 cgtctggaag gtaacaacgc cgaactcggc gcgaagaaac tggctgacag cggcctgaat
     5521 attattgcag caaaaggtct gacggatgca gctcagcagg ttgttgccgc agtggagggg
     5581 aaataatgtc cattttaatt gataaaaaca ccaaggttat ctgccagggc tttaccggta
     5641 gccaggggac tttccactca gaacaggcta ttgcatacgg cactaaaatg gttggcggcg
     5701 taaccccagg taaaggcggc accacccacc tcggcctgcc ggtgttcaac accgtgcgtg
     5761 aagctgttgc tgctactggc gctaccgctt ctgttatcta cgtaccagca ccgttctgca
     5821 aagactccat tctggaagcc atcgatgcag gcatcaaact gattatcacc atcactgaag
     5881 gcatcccgac gctggatatg ctgactgtga aagtgaaact ggacgaagct ggcgttcgta
     5941 tgatcggccc gaactgccca ggcgttatca ctccgggtga atgcaaaatc ggtatccagc
     6001 ctggtcacat tcacaaaccg ggtaaagtgg gtatcgtttc ccgttccggt acactgacct
     6061 atgaagcagt taaacagacc acggattacg gtttcggtca gtcgacctgt gtcggtatcg
     6121 gcggcgaccc gatccctggc tctaacttta tcgacatcct cgaaatgttc gaaaaagatc
     6181 cgcagaccga agcgatcgtg atgatcggtg agatcggcgg tagcgctgaa gaagaagcag
     6241 cggcgtacat caaagagcac gttaccaagc cagttgtagg ttacatcgct ggtgtgactg
     6301 cgccgaaagg caaacgtatg ggtcacgcag gtgcgatcat tgccggtggt aaagggactg
     6361 ctgatgagaa attcgctgct ctggaagccg caggcgtgaa aaccgttcgc agcctggcgg
     6421 atatcggtga agcactgaaa actgttctga aataaatatc tgtaataaga aatagccctc
     6481 gccgcttccc tctacaggag gggcgaaggg ctgtcggttt cgacatggtt ggccatctta
     6541 tgatggcctt ttttattcgg gcactaatct gaaaagtttg cccactgctt ttctcccatc
     6601 ttctgcgcaa taatgcccgg caaaatatcc cctctccgtt ctgtaaaagc atttatacaa
     6661 gaagaaaatt gttctgccgg ttgctctggc cctgatgggt gtaaattcgg acgtttatgc
     6721 ggccgatgta aatatcgata ttttaagtgc gacagtgaaa gataaacgca ttgagggagt
     6781 atcggtgacg ctgcaacgca atggcgcaca atctgtttct ggaaccacaa atgcatcggg
     6841 cagcgttaat ctgggttcta catttgctga tgatcaagat gctttattga tcgtcaaaaa
     6901 agagggctat tctaatctgg tggtcaaatg ttcgtgtgct ggcatgactt atgcgattag
     6961 tccggcaatg actagtctgg atggcatgcg tgtagttttg agctggggcg aaaagccttt
     7021 tgatcttgat tcacatttga tcttccctgg cggacatatc tattttgata gtaaagaggg
     7081 gactgatgct aatctggatg ttgatgatac ggatagctat ggatcagaaa ctgtcactat
     7141 cagcaaaaag cattttggcg aaagttacat ctatgccgtg caggactata gcaacaaagg
     7201 gttgcccaac agtaattatc tctctgccag taaggcaaaa gtgtttgttt atgtagggag
     7261 ctccctggtg cgtagttata gtgtccctgc tggaaaacga gggaacatct ggaccgtgtt
     7321 taaactcaat ccaaacggcg agtttgaaga tatcaactct gttaccagcg ccaattttaa
     7381 cgacaccaca ttaggcgtca gggatttagc cactgtgata atgcctgcga cgggttcttc
     7441 aactccggct tcacctgcga tgcaaaacag tggagatact caactggcaa gaaaatataa
     7501 tcgtgaaggc gaagctgttt acaaaaccgg tcaacttgag caagccattc aactcttcca
     7561 acaggcaaca gaattagacg gcaattacgg acaggcattt agtaacctgg ggctggcgta
     7621 tcagaaaaat ggcaacatcg cggaagcaat ttgggcaaat cgtaaagcga tctcgttagc
     7681 cagtggagca aatgcggcga caacgcgcgc taactcctat tataacattg ccaaaattta
     7741 tgaaac
//
LOCUS       DADSZD010000181         7639 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.182, whole genome shotgun sequence.
ACCESSION   DADSZD010000181 DADSZD010000000
VERSION     DADSZD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.182"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            127..576
                     /gene="alaE"
                     /locus_tag="J1Z28_003805"
     CDS             127..576
                     /gene="alaE"
                     /locus_tag="J1Z28_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBA7718598.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(613..957)
                     /locus_tag="J1Z28_003806"
     CDS             complement(613..957)
                     /locus_tag="J1Z28_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBA7718599.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1109..1438
                     /locus_tag="J1Z28_003807"
     CDS             1109..1438
                     /locus_tag="J1Z28_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBA7718600.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1495..2829)
                     /locus_tag="J1Z28_003808"
     CDS             complement(1495..2829)
                     /locus_tag="J1Z28_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBA7718601.1"
                     /translation="MPRYQDIARQLKTAIEQGELKPGARLPSSRTWSQELGVSRSTVE
                     NAYAELVAQGWLIRRGQAGTFVSERIYPQQSTVQVVDFAGESQQPLPFQMGLPALDLF
                     PRELWARVMGRRLRTQTRFDLALGDVCGEVALREAIVDYLRVSRGIDCQPEQVFITHG
                     YAASIALILHALAKPGNGMWIEDPGFPLIRPIVTRHDVEILPVPVDDNGLDITSGIQN
                     YPDARFALITPAHQSPLGVALSLARRHQILEWADRSQAWIIEDDYDSEFRYHGKPLPA
                     LKSLDAPQRVIYAGTFSKALFPALRCAWLVVPVKQIAQFRHQASLAPCAVPVLWQNTL
                     ADFLREGHFWRHLKKMRQHYAQRRQWIEQALTQQGFQVVPQKGGIQMVIRMIGDDIAH
                     ARKANAAGLAVQALSDWRIRSSGEGGLLLSFTNIVNEGMARQVAQQLRKALS"
     gene            2917..3348
                     /locus_tag="J1Z28_003809"
     CDS             2917..3348
                     /locus_tag="J1Z28_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBA7718602.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3557..3802
                     /gene="nrdH"
                     /locus_tag="J1Z28_003810"
     CDS             3557..3802
                     /gene="nrdH"
                     /locus_tag="J1Z28_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBA7718603.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3799..4209
                     /gene="nrdI"
                     /locus_tag="J1Z28_003811"
     CDS             3799..4209
                     /gene="nrdI"
                     /locus_tag="J1Z28_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBA7718604.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4182..6326
                     /gene="nrdE"
                     /locus_tag="J1Z28_003812"
     CDS             4182..6326
                     /gene="nrdE"
                     /locus_tag="J1Z28_003812"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBA7718605.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDSQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISERYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IHSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQSNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6336..7295
                     /gene="nrdF"
                     /locus_tag="J1Z28_003813"
     CDS             6336..7295
                     /gene="nrdF"
                     /locus_tag="J1Z28_003813"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBA7718606.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNLEKISQARREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWSDDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         1910 a         1951 c         1873 g         1905 t
ORIGIN      
        1 aaaatttttc actaattgat tagtcatagc cagcgatata cgctatgcga aaatgcagat
       61 ggcaatgaga tccactgctt tcatctccat taacatccca ttacgctttt attaaggagc
      121 attagcatgt tctcaccgca gtcacgcttg cgtcatgcag ttgcagatac gttcgcgatg
      181 gttgtttact gttctgtcgt gaacatgtgt attgaagttt tcctctccgg aatgagcttc
      241 gaacagtctt tttattccag attggtagcg attccggtga acatcttaat tgcatggcca
      301 tacggtatgt accgcgatct gtttatgcgc gcggcacgca aagttagccc gtcgggctgg
      361 ataaaaaatc tggcggatat cctggcttat gtgacgttcc agtcaccggt gtatgtggcg
      421 atcttgttag tggtgggcgc agactggcat cagattatgg cggcggtcag ttcaaacatc
      481 gttgtttcga tgttgatggg ggcggtttat ggctacttcc tcgattattg ccgccgactg
      541 tttaaagtca gccgttacca gcaggtaaaa gcctgaccct tcttttcgcg actggcatcg
      601 ccagtcgcgt ctttaactgg cttcgccaaa taaaccattc aaataacgtt caagcgcaac
      661 acgcgaacta aagccatgcg ggataccgta atgctcatga cgttcacccg ctaaatagag
      721 cggaaattgc tggtaatgat cgcaagtttt aatatcggaa gcgggttcag ccagtgttga
      781 actacgttct tttaatgttg gatgaatcac cagggcggta cgtcccattc gcgcttcgcg
      841 attaacatag acataatttt ccccacggcg gtaaccatac gctttctggg ttaccacatc
      901 gacagtaaaa ccgacttttt caagtacgcg cgccacctcg tctggtcgta aatacatatt
      961 tgatcctcgt tattatcaac cgcgggccta ccttacctga ttgcgcattc acaacgcttt
     1021 cagaaaagtc cataaagccg cgatgaacct cagttaagaa aattatggtc tacactgaaa
     1081 attacatcga attctaatgg gggatcatat gtttaaccga ccgaaccgca acgacgttga
     1141 tgatggcgtg caggatatcc agaatgatgt caatcaatta gctgacagtc tcgaatctgt
     1201 attgaagtcc tggggcagcg acgccaaagg ggaagccgaa gccgctcgta gtaaagcgca
     1261 ggctctactg aaagaaacca gggcgcgaat gcatggtcgt actcgcgtcc agcaagccgc
     1321 gcgcgatgcc gttggctgcg cggattcttt tgttcgtgaa agaccctggt gtagtgtggg
     1381 tacagcggca gcggtgggta tttttatcgg cgcactgtta agcatgcgca aatcgtagtg
     1441 cacaaatgat aataaatacg cgtctttgac cccgaagcct ggcttcgggg ttttttagct
     1501 taaggcttta cgtaattgtt gtgctacctg tcgcgccata ccttcgttaa cgatattagt
     1561 aaacgaaagc agtaatccac cttccccact tgaacggata cgccagtcgc taagtgcctg
     1621 caccgcaagg cctgcagcat tggctttacg cgcatgggca atatcatcgc ctatcattct
     1681 gatcaccatc tggataccac ctttctgcgg cacaacctga aatccttgct gcgtcaacgc
     1741 ttgctcaatc cattgccgac gttgagcata atgctgacgc attttcttca gatgccgcca
     1801 gaaatgcccc tcgcgaagaa agtctgccag tgtgttctgc catagaacag gtaccgcaca
     1861 tggagccagt gacgcctggt ggcggaattg tgcaatttgc ttcaccggca ccaccagcca
     1921 cgcacagcgc aatgcaggaa atagcgcttt gctgaatgtt ccggcataaa ttacccgctg
     1981 cggtgcgtcg agacttttta acgccggtaa cggcttaccg tgatagcgaa actcactgtc
     2041 ataatcatct tcaataatcc atgcctgact acgatctgcc cattccagta tctgatgcct
     2101 gcgcgctaaa gagagcgcca cacccagcgg actttggtgt gctggtgtta tcagggcaaa
     2161 acgcgcatca ggataatttt gtattccgct tgtgatatcc agtccattgt catcaaccgg
     2221 cacaggcaaa atttccacat catggcgagt gacaatcggg cgaatcagtg gaaagccggg
     2281 atcttctatc cacatcccgt ttcccggttt cgccagcgcg tgcagaatta aagctattga
     2341 ggccgcataa ccgtgagtga taaagacctg ctctggctga caatcaatcc cacgtgaaac
     2401 gcgtaagtaa tcaactattg cctcgcgcaa cgccacctca ccgcaaacat cgcctaacgc
     2461 caaatcaaag cgcgtctggg tacgaaggcg acgccccatc acccgcgccc acagctctcg
     2521 cggaaagaga tcgagtgcgg gtaatcccat ttgaaatggc aacggttgct gactttcacc
     2581 ggcaaaatct acaacttgta cagtagattg ttgcggatat atccgctcac tgacaaatgt
     2641 gccagcctgt ccccgcctga tcagccatcc ttgcgccacc agctcagcat acgcattttc
     2701 gacagtggat cgagacaccc ccagctcctg cgaccaggta cgacttgaag gcagccttgc
     2761 gccgggtttc agttcccctt gctcaatagc cgtttttaac tgacgggcga tatcctgata
     2821 gcgcggcata tggtctgctt ttttaggtaa tttatggctc tctttagcag tccattatag
     2881 tgctacattg cgggcgttaa taagaggaga aactgtatga ctacattacg tcagccttac
     2941 tacgaactta gcccggcagt gtataacgca ctagtgcagg ccaaaacggc actggaaaat
     3001 agcacgctgg ataccacgtt gatggagtta gtttatttgc gcgtctcgca aatcaacggc
     3061 tgcgcatttt gtctggagat gcacagcaaa gcattgcgca aatccggcgt gccacagcac
     3121 aaactggacg ccctggcagg ttggcgcgta agccatcatt ttgatgaacg cgagcgggcg
     3181 gcgctggcat gggcggaatc ggtaaccgaa attgccagaa cccatgcgga agacgaggtt
     3241 tatcagcctt tgcttgagca tttcagcgca gcagagatca gcgacttaac gtttgccatc
     3301 gggctgatga attgttttaa ccgtctggcc gtttccatgc ggatgtaact tcttgatgcc
     3361 gggtatttgc ccggcaaaaa aacacaaaat cccctacccc gttgagccat tcatcggtgg
     3421 cgtaatcaat ctaaaattcc catatcttgt gtggttgaat cttttttcaa ctacatctag
     3481 tatctctgta tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta
     3541 aacggaaata cgaatcatgc gcattactat ttacactcgt aacgattgcg ttcagtgcca
     3601 cgccaccaaa cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg
     3661 cgttcctgaa gcggcagaag cgctgcgtgc tcagggcttt cgtcagttgc cggttgtgat
     3721 tgcaggcgat cttagctggt ctggtttccg cccggacatg atcaatcgtc tgcatccagc
     3781 gccacacgcg gccagtgcat gagccagctc gtctactttt ccagcagctc cgaaaatacg
     3841 cagcgtttta tcgaacgttt agggctgccc gcggtgcgca tcccgctcaa tgagcgggaa
     3901 cggattcagg tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg
     3961 gctggcgcgg tgccacgaca ggtcattcgc tttttaaacg acgagcataa ccgggcgttg
     4021 ctgcgcggcg ttattgcttc gggtaatcgc aactttggtg aggcatatgg ccgcgccgga
     4081 gatgtgattg cccggaaatg cggcgtgccg tggctgtacc gctttgaact catgggtacg
     4141 caaagcgata tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag
     4201 aacgcctgac gcaggaaacg atggattatc acgctctgaa tgcgatgctt aacctttacg
     4261 atagcgcagg tcgcattcag ttcgataaag acagccaggc agtagacgcc tttatgacga
     4321 cgcatgtgcg tccgaacagt gtggccttca gtagccagca gcagcgcctg aactggctgg
     4381 ttaacgaagg ttactatgat gaaagtgtcc ttaatcgcta ctctcgcgat tttgtcatta
     4441 cgctgtttgc ccacgcgcac gccagcggtt ttcgtttcca gacattccta ggagcatgga
     4501 agttttacac cagctatacg ttgaagacat tcgacggtaa gcgttatctg gaagattttg
     4561 ccgatcgcgt cacgatggtg gcgctgacgc tggcacaagg cgatgaaaca ctggcgacgc
     4621 agctcactga tgagatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact
     4681 gcggtaagca gcagcgcggc gaactggttt cctgttttct gctgcgtatt gaagacaata
     4741 tggagtcgat tggccgggca gtaaattccg cactacaact gtcgaaacgc ggcggcggcg
     4801 tagcattttt gctgtctaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc
     4861 aatcttctgg cgtgattccg gtgatgaaaa tgctggaaga cgcattttcc tatgccaacc
     4921 aactcggcgc gcgtcagggg gctggggcag tctatttaca tgctcatcat cccgatattc
     4981 tgcgttttct cgatacaaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt
     5041 cgctgggcgt ggtgatcccg gatatcactt tccatctggc aaaagagaat gctcagatgg
     5101 cgctgttttc gccctatgac gtagagcgag tttatggcaa gccgtttgcc gatgtcgcca
     5161 tcagcgaacg ctatgacgaa ctggttgccg atgaacgcat tcgcaaaaaa tacctcaacg
     5221 cccgtgattt cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca
     5281 tgtatgaaga cacggttaac cgtgctaacc ctatcgccgg gcgcataaat atgagcaatc
     5341 tctgctcaga aattttgcag gttaacagtg cctcagagta tgacgagaat ctcgactatg
     5401 cccgtacagg ccatgatatt tcctgcaatt taggttcgtt gaatattgcc cacaccatgg
     5461 attcccccga ctttgcccgc acggtagaga ctgccgtacg cggtttaacg gcggtgtcag
     5521 atatgagtca tatccacagc gtaccgtcca tcgaagccgg aaatgccgcc tcacacgcca
     5581 tcggtctggg gcagatgaat ttacacggtt atctggcacg agaaggcatc gcttatgggt
     5641 cgccggaagc actggatttc accaatctct atttctatac catcacctgg cacgcactgc
     5701 gtacctcgat gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac
     5761 gctatgccag tggtgaatat ttcagccaat atctgcaaag taactggcaa ccgaaaacgg
     5821 cgaaagttgg cgaactgttt gcccgtagtg gtattacgtt acctacccgt gagatgtggg
     5881 cgcagctgcg cgacgacgtg atgcgctatg gcatatacaa ccagaatctc caggcggtgc
     5941 cgccaaccgg ttctatctct tatatcaacc atgccacgtc gagtattcat ccgattgtgg
     6001 cgaaagtaga gatacgcaaa gagggcaaaa ccgggcgcgt ttactaccct gccccgttta
     6061 tgactaacga aaatctggcg ctgtatcagg acgcttacga aatcggcgca gaaaagatca
     6121 tcgacaccta cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt
     6181 tccccgatac cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaagg
     6241 gtatcaaaac gctctattac attcgcctgc gtcagatggc gctggaaggc actgaaattg
     6301 aaggctgcgt ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca
     6361 tcaactggaa caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca
     6421 atttctggct accagaaaag gtgccactgt cgaacgatat acctgcatgg cagacattaa
     6481 gtgtcgtaga acaacaactg acaatgcgcg tttttactgg cctgacgctg ctcgacacgc
     6541 tgcaaaatgt tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg
     6601 cagtattatc gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcaattt
     6661 tctcgacgct gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg
     6721 cgccgttgca gcgaaaagca cagattattc agcaacatta tcgcggtgat gatccgctga
     6781 aaaagaaaat cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc
     6841 cgatgtattt ttccagccgc ggaaagctaa ccaataccgc agacctgatc cgtctgatta
     6901 tccgggatga agcagtccac ggttactaca tcggctataa atatcagaaa aacctggaaa
     6961 agatctccca ggcacgacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac
     7021 tctatgacaa cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tggtctgacg
     7081 atgtaaaagc gtttctctgt tacaacgcca ataaggcgtt gatgaatctg ggctacgaac
     7141 cgctatttcc cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga
     7201 atgccgatga aaatcacgat ttcttttccg gttcaggctc ttcatatgtg atgggaaaag
     7261 cggtcgaaac agaagatgaa gactggaatt tctgagggtg ttatttttca aaaatatcac
     7321 tacccgcagc agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattttgtgg
     7381 tgatctacac tgatactctg ttgcattatt cgcctgaaac cacaatattc aggcgttttt
     7441 ttgctatctt tgacaaaaaa tatcaacctt tctcgatttg ctctcagccc ttatatcatg
     7501 ggaaattccg gcgatttgct cacatcaata ttcatgccac atttgccatc aggggttgcc
     7561 tcagattctc agtatgttag ggtagaaaaa agtgactatt tccattgggt aatgtatcga
     7621 cataaacaaa taacaggaa
//
LOCUS       DADSZD010000182         7618 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.184, whole genome shotgun sequence.
ACCESSION   DADSZD010000182 DADSZD010000000
VERSION     DADSZD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.184"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            2..214
                     /locus_tag="J1Z28_003814"
     CDS             2..214
                     /locus_tag="J1Z28_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBA7718607.1"
                     /translation="MNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQQF
                     PVRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(430..1359)
                     /gene="fdhE"
                     /locus_tag="J1Z28_003815"
     CDS             complement(430..1359)
                     /gene="fdhE"
                     /locus_tag="J1Z28_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBA7718608.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1356..1991)
                     /gene="fdoI"
                     /locus_tag="J1Z28_003816"
     CDS             complement(1356..1991)
                     /gene="fdoI"
                     /locus_tag="J1Z28_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBA7718609.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1988..2890)
                     /gene="fdxH"
                     /locus_tag="J1Z28_003817"
     CDS             complement(1988..2890)
                     /gene="fdxH"
                     /locus_tag="J1Z28_003817"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HBA7718610.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2903..5953)
                     /gene="fdnG"
                     /locus_tag="J1Z28_003818"
     CDS             complement(2903..5953)
                     /gene="fdnG"
                     /locus_tag="J1Z28_003818"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5366..5368),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBA7718611.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPSSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6147..6980
                     /gene="fdhD"
                     /locus_tag="J1Z28_003819"
     CDS             6147..6980
                     /gene="fdhD"
                     /locus_tag="J1Z28_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBA7718612.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQMFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7068..7556
                     /locus_tag="J1Z28_003820"
                     /pseudo
     CDS             7068..7556
                     /locus_tag="J1Z28_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
BASE COUNT         1748 a         1988 c         2033 g         1849 t
ORIGIN      
        1 gatgaatacg gtttttcttc atttatcaga agaggcaatt aaacggctga acaagcttcg
       61 gggatggcgt aaggtttcgc gctctgcaat tttacgcgaa gcggtagagc aatatctgga
      121 gcggcagcaa tttccggtgc gtaaggcaaa aggcggcagg caaagggacg aggcagttgg
      181 tgttgaagaa ctatgtaagc agcataagga atgacaaaag tttttttatt catgaatata
      241 aaaaaccaga tgcccttatt ctggtattaa tacaaggctg ttttacttga acttataata
      301 actgcaactg ttacatcata tctggaaaac gcctcgcgaa atacgagaga ttttgcggga
      361 atagcaggtg tcgtcacacc ctatgagctg taatccgggc gaccagcgcc acccggaaaa
      421 cgtcagagat tactcccctt cacccggaaa caggaacggg ttaatggaac tacgggcata
      481 gccttcttgc tccattcgcg cgtccagtac cagagaggcg aggtcatctg ccacggcttc
      541 aactttcggt tctttttcct gataaagaat cttcaggtaa gtgccgcagt catcgcagct
      601 ttcggcttta atcgcggcct gttcgtcatc cagcgaccag taatgcagtt tgccgctttg
      661 ttcacagttg ctgcatttta cgcgcactac gtgccattca gtttcacaca ggttgcagtg
      721 caggtaacgc agaccctgag tggtgccaat ttgcaccatg ctggacaccg gcatagagcc
      781 acaaaccggg caatattgac gttgttcgcc gtattcagcg cgggctttgc cggggatcag
      841 attagccatc tgcgcccagt agagcgacag cgcagcccag ataaacggcg ctttatcgct
      901 gctgacggac gagaaatcag aggcaaacag tgcgctggcc atatcttcca gctcctgagt
      961 cgatgccttc tccagattct caatcactgc cagcgccggg ccgctcattt caggtttcag
     1021 ctcagcaatc agcgccatca gcagcttttg ccagtgctta tcacgcggca gaacgtgaat
     1081 atccagcggg ggcttgcctt gtgcgctggc ttctttaatg cgcgtggtca gatccatctc
     1141 cagcggatgg tcgtacagca ccacttcctg ggcgtgggcg ataagcgcag caaagcgcag
     1201 gtaatcaccc agcggattat tttctgccag ctcgcgcaga cgctcggcgc ggcggttgta
     1261 taaattcttg agccgaggga acaataacgg cggaatcata tccgccgtac gtttctcgct
     1321 cgaacccagc tcatcttgcg ggattatgcg aatactcatt cagctttctt ttccgttgtc
     1381 ttgcggacct cacggtacca gcgcgggtga tgtttcttcg cccaggcgct ggtcacccat
     1441 ccttccacca tggcggtaat cgtgcctttc acccaaaggg cggcgtagat atgcaccatg
     1501 ataaccacaa ttaacgccac tgcggcaaat gaatgcagca ttaacgcgaa tcggatcacc
     1561 gggattgaga aagcaggcgc aaaataagga cgccagataa tcacgccgct caccagcagc
     1621 agaaccagga aaataatcgc cgcccagaaa acgcatttct gaccgaagtt ataacgcccg
     1681 gtgtcaccta cttcctcgtt gacgacgatc ttacgaatat tcttcgccca aaagatatca
     1741 tcccgattga ttaggttgtg atgccagtaa cggaaaaaca tgatgatgaa cgaggcaaac
     1801 ataaccacgc cgacaaacgg gtgcagaatg cgcgccagct gcggtgtgcc catgatttgc
     1861 atcaaccagt tgaaggacgg gaacaaaaag cccagcccgc tcaccgccgc caggatgaag
     1921 cagaaggcgg tgatccagtg gttgatacgt tccggcgcgg tgtagcgcac gatggtgtca
     1981 cgtcgtttca tttgcgctcc tcgtctttct cttcgtgcag attattctct tcctcatccg
     2041 cacggttcgg accgacaccc acgtagtgga agatactggc tgcgaaggta gccgcaaagc
     2101 caacagctgc gagcggtttc cagatgcctt tccagaattt cacggtttcg ctgatttccg
     2161 ggttctccgg caagccatga tacagatttg gcttgtcagc atggtgcagc acgtacataa
     2221 catgtgtacc accgacgcct gccgggtcgt acaaacccgc attgtcgtaa ccacgggttt
     2281 tcagctccgc cacgcgctcg ctcgccagcg ttttcatcga ctctttcgta ccgaagtgaa
     2341 tcgcgcccgt tgggcaggtc ttcacgcagg ccggttcttg cccaaccacc acgcggtcaa
     2401 cgcacaacgt acatttgtag acgcggttgt cttccgggtt gaggcgcgga atgtcgaacg
     2461 gacagcccgc aatgcaataa ccgcagccga tgcactgctc ggactggaag tcgacgatac
     2521 cgttggcata ctgaataatt gccccttccg ccgggcacgc tttcaggcag cctggatcgg
     2581 aacagtgcat acagccgtct ttgcggatca gccattccag tttgtcgttc tgctccactt
     2641 ccgagaatcg catcaccgtc cacgatttgg cacttaaatc attggggttg tcgtacaccc
     2701 caatgttatt gccgacggta tcgcgaatgt cgttccactc tgaacacgcc acctgacagg
     2761 ctttacagcc gatacaggtg gtaacgtcga tgagtttcgc cacttcttcc tggaagtccc
     2821 gcgcctgagg cgcgggggtc agaccgttag tcgcggaacg acgaatgata tcttgcgatt
     2881 gataagccat atgtcgtctc cgttacacct tttccacatt cacaaggaag gacttaaact
     2941 ccggcgtctg cgtgttcgca tcaccgacga atggcgtcaa cgtattggca ataaagcctt
     3001 ttttcgcaac accttcatag ccccagtgaa tcggaatacc gatggtatcg atatctttgc
     3061 cgtttgcttt cagcgtgcga atacgtttgg tcactaccgc tttggctttg atatagccac
     3121 ggttggagga gactttcaca gtatcgccct gggcaatgcc aagtttattc gccagcgact
     3181 ccccgatttc cacaaactgc tctggttgca aaatcgcatt caacagcgcg tgtttggtcc
     3241 agtagtggaa gtgctcggtc agacgatagg tggttccgac atacgggaac ttatcggctt
     3301 tacccaatgc ttcggcgtcg tctttaaaga tacgcgccgc cgggttcgag ataacgtttg
     3361 gatgcagcgg gttagttccc agcggcgttt caaacggctc gtagtgttcc gggaacggac
     3421 cttccgccat cttatcgagg gcaaacagac gtcccatgcc ttcctgctgc atgataaacg
     3481 gccctacgcc gctgcccgga ggcgctgcgc tgtagtccgg aatatcccag ccggtccact
     3541 tagtgccgtc ccatttcagc aactgacgct tcggatccca tgggttaccc tgcggatctg
     3601 cggaggcgcg gttatacaga atgcggcggt taagcggcca tgcccatgcc cagcccagcg
     3661 tgttaccgag gccagacgga tcggcgttat cacgacgcgc catctggttg ccttccggcg
     3721 tccagctacc ggcgaaaatc cagcagccac aggaggttgt accgtcatcg cgcagttggg
     3781 cgaacgagct aagttgttgg cctttcttga cgataaccgc accggttgcc gggtcggtaa
     3841 tatcggccag cgctttgccg ttgctctcca ttgccacttc ttctgaagat ggctcatgcg
     3901 gaatggcgta gttccaggtc atgttcagca cctggtccgg gttcgcgcca ccctgttcgg
     3961 catacatctt gcgcaagcgc aggaagatac cggagaggat ctcgccatca gtcagcgcaa
     4021 tccccggggc gtccgcacct ttccagtgcc attgcaacca gcgaccagag ttaacgattg
     4081 aaccgttctc ttccgcgaag caggtcgatg gcagacggaa cacttcggtc tggatcttcg
     4141 acgagtcaac ttcgttcagc tcaccgtggt tctgccagaa gttagaggtt tcagtgttca
     4201 gcgggtcgat agtcacgagg aatttcagct tcgacagaca gccgatcact ttgtttttgt
     4261 tcgggaatga ggcaacaggg ttaaagccct ggcagatata gccattgacc ttgccctctt
     4321 tcatcatctc gaagtattgc aggacgtcgt agcctttatc ccacttcggc aaccagtcaa
     4381 agccccagct attttccgcc gtcgctttat caccaaagaa ggccttcatc attgagacga
     4441 agaatttcgg gtagttgccc cagtagttga cctggccttc cagcagtggt tttggcgtgt
     4501 tggcagcgag gtaagtttgc agatcggtct gcttctcgct tggcaacgtc atgtaacctg
     4561 gcagactctg cgacagtagc cccaggtccg tcagcccctg aatattggag tgaccgcgca
     4621 gggcgttaac gccgccgcca gccatcccca tattgccgag cagcaactgg atcatcgcca
     4681 tcgtacggat gttctgcgca ccgatggagt gttgcgtcca gccgagggcg tagaggaacg
     4741 acgctgtttt atcgtgagca ctggtttcgg cgatgtattc gcagactttc aggaacgcgt
     4801 cttttggcgt accgcagatg ttttcaacca catctggcgt ataacgggaa acgtgctgtt
     4861 tcagcaagtt ccacacgcaa cgtggatgtt gcagggtagt atcacgtttg gcgaagccgt
     4921 tttcatccag ttcgtaagtc caggtggact tatcgtactt gcgtttttcc gcgtcgtagc
     4981 cggtgaacag gccatcttca aagccgtaat cctcacgcac gatcaggctg gcgttggtat
     5041 aggcttcggt gtattcgcgg ttgaattttt cattgttcag caggtacagc aatacgcctg
     5101 acaggaaagc aatgtcagta ccggaacgga taggggcata gtagtcagcc accgccgctg
     5161 tacgtgtaaa gcgaggatcg atcacaatca gcttcgcgcc gttgtgaatt ttggcttcca
     5221 tcgcccagcg gaacccgacc gggtgagctt cagcggcgtt accgcccatc accacgacga
     5281 ggttggcatt cttgatgtcg acccagtggt tggtcatcgc accgcgacca aatgttggag
     5341 caagacttgc taccgttggt ccgtgtcaga cacgcgcctg gttgtcgacc gcgagcatac
     5401 ccagcgcgcg ggagaatttt tgcgttaaat agccggtttc gttactcgac gcggaagcac
     5461 acagcatccc ggtggagagc cagcggttaa cagtcacgcc ttcggcgttt tgcgcaatgt
     5521 agttcgcatc gcggtcttct ttcatcagtt tggcgatgcg atcaaacgcc tcttcccaac
     5581 tgatttgttg ccatttatca gaacctggcg cacggtattc cggaaacttc aggcggcttt
     5641 cggagtggat gaaatccacc agaccagcgc ctttcggaca aagtgctccg cggttgaccg
     5701 ggtgatccgg gtcaccttcg atatggaaga tagatgcttt ggcgtttttt gctccgtcac
     5761 cgaggctgta catcaacagc ccacagccta cggaacaata ggtgcaggta ttacgggttt
     5821 cgcgggtgcg cagcagttta tactgccggg tttccgcgag tgctacgctg ggtgcaaaac
     5881 ccagtgccgc tgccgtggtg cctgccatac cgccagcgca gatcttaaag aactgccttc
     5941 tgctgacctg catggattgc tccttgtttc gacattgtca cgtcccattt acattcgctt
     6001 gctgcgtgtg cagggagtgg gagttatttt tctttgcgga aggggccgca aaggtccaga
     6061 attggctcaa tttccctcca tccaggaagg gtttgtaaca gaataccata atgttggtgt
     6121 gtgtgttctt atctggttaa tagaaagtga aaaaaacaca gcaaaaagaa atcgagaatg
     6181 tgacaaatat cacaggtgtt cgtcaaattg agttatggcg gcgtgatgat ttacaacacc
     6241 cacggttaga tgaggtggcg gaagaagttc ccgttgcgct ggtctacaac ggcatttcgc
     6301 acgtggtgat gatggcgtcg cccaaagacc ttgagtactt tgcgctcggt ttttcgcttt
     6361 ccgaaggaat tatcgaaagc ccgcgcgata tcttcggcat ggatgtcgtt ccttcctgta
     6421 atggcctgga agtacaaatt gagctttcca gccgccgctt tatggggttg aaggagcgcc
     6481 gccgggcgct ggcgggacgt acgggctgtg gcgtatgcgg tgtagaacag cttaacgata
     6541 tcggtaaacc ggtgcagccg ctgccgttca cccagatgtt tgatctcaac aaactggatg
     6601 atgcattacg tcatctcaat gatttccagc cagtggggca actgactggt tgtactcacg
     6661 ccgctgcatg gatgttgcca tctggcgaac tggtcggcgg gcatgaagac gtgggtcgcc
     6721 atgtggcgct ggacaaactg ttaggccgcc ggtcacaaga aggggaaagc tggcaacaag
     6781 gtgcggtgct ggtttccagc cgtgccagtt atgaaatggt gcaaaagtcg gcgatgtgcg
     6841 gcgtagagat tttgtttgcg gtgtctgccg cgaccacact tgctgtagaa gtggctgagc
     6901 gctgtaatct gacgctggtg ggcttttgta aaccgggtcg ggcaacggtt tatacccatc
     6961 cgcagcggtt gagcaattaa ttaaaataag tcctgtgata tatattaaat tgcaggactt
     7021 attcatttcg tgaattttgt tattttttca taaacaaaac gcgtgtagtg gcgttaacgc
     7081 ctgcgctgca acctattgat ggcgtggcgg tgagttatat tgatgctgcc gtagcgttgg
     7141 gtaataccat taacgaaatg gataaatatt acacccagga aaattataaa gacgatgcgt
     7201 ttgcgaaagg gaaaacattc cactactact ctctggcagt catgatttca gcgaaacaaa
     7261 ttaataacct gatatcgcaa gataagtttg atgcagaagc ggcaatgaag aaagtgtctg
     7321 aactggaaac gctggtggcg caagccaaag aagcggataa aagcggcatg aatttctcgt
     7381 ttattaattc ggcaggccag tatcaactcg aggctaaaaa atacgttcgc cgcatcagag
     7441 ataaagtcct gcactctgac tgggataaag agcaacttca ggacgcaaac tcaagctgga
     7501 tggccgaaga ctcatttcca cgagcgttat gcgattacaa cgaaatggtc gatgaaatat
     7561 tacagttaat tgtaatcgcg gggcggtttt gtgatgagca cggatatgtc actaagtc
//
LOCUS       DADSZD010000183         7587 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.185, whole genome shotgun sequence.
ACCESSION   DADSZD010000183 DADSZD010000000
VERSION     DADSZD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.185"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..194)
                     /locus_tag="J1Z28_003821"
     CDS             complement(<1..194)
                     /locus_tag="J1Z28_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718613.1"
                     /translation="MCPECFFLMLFFCGYRACYCSSSSSFRSSPVYGFSGRPPGGAGC
                     RERSQRSCLRPGGLPSLTRNP"
     gene            complement(313..504)
                     /locus_tag="J1Z28_003822"
     CDS             complement(313..504)
                     /locus_tag="J1Z28_003822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718614.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(501..923)
                     /locus_tag="J1Z28_003823"
     CDS             complement(501..923)
                     /locus_tag="J1Z28_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBA7718615.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWNLSD
                     DELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENRGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(971..1273)
                     /locus_tag="J1Z28_003824"
     CDS             complement(971..1273)
                     /locus_tag="J1Z28_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA7718616.1"
                     /translation="MMPEYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(1369..1811)
                     /locus_tag="J1Z28_003825"
                     /pseudo
     CDS             complement(1369..1811)
                     /locus_tag="J1Z28_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001450806.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1472 domain-containing protein"
     gene            complement(2290..3261)
                     /locus_tag="J1Z28_003826"
     CDS             complement(2290..3261)
                     /locus_tag="J1Z28_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HBA7718617.1"
                     /translation="MKRAPVIPKHTLKTQPLEDTSSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGIIEIADGSRRRKAAALTESDFRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKTAL"
     gene            complement(3261..4427)
                     /gene="sopA"
                     /locus_tag="J1Z28_003827"
     CDS             complement(3261..4427)
                     /gene="sopA"
                     /locus_tag="J1Z28_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HBA7718618.1"
                     /translation="MGLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIK"
     gene            complement(4890..5087)
                     /locus_tag="J1Z28_003828"
                     /pseudo
     CDS             complement(4890..5087)
                     /locus_tag="J1Z28_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="controlled cell death protein; post-segregation
                     toxin; toxin addiction system; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
     gene            5209..5967
                     /locus_tag="J1Z28_003829"
                     /pseudo
     CDS             5209..5967
                     /locus_tag="J1Z28_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(6356..6807)
                     /locus_tag="J1Z28_003830"
                     /pseudo
     CDS             complement(6356..6807)
                     /locus_tag="J1Z28_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            6960..7349
                     /locus_tag="J1Z28_003831"
                     /pseudo
     CDS             6960..7349
                     /locus_tag="J1Z28_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1969 a         2063 c         1786 g         1769 t
ORIGIN      
        1 gggtttcgcg tcagggatgg aagcccgcca gggcggagac agcttcgctg gctccgttca
       61 cgacagcccg ccccgccagg gggacgcccg gaaaagccat aaacaggaga ggaacggaaa
      121 gaggaagaag aagaacagta acaggcacgg taaccacaga aaaataacat cagaaaaaaa
      181 cattccggac acatcacaaa caggaagggt aacgggcgac gcgtcagcgt ggcccgcttc
      241 ccttccggca ggcgtcagcc tgccaggggt tttcgcgcgg gctgacaggc ggcctccacc
      301 gcctgccctg tatcacctgt ttctgctgcc ctgtaagatg tagtgaccgc ccaggaaaac
      361 agcctgctga ttcgccacaa aatcgcagat ttcatcaaca atctgcgcag tggtgagcgg
      421 tttctgcccc atttcacgac gccgcgcatt aatcacggct ttgtaattgc gcagaatttc
      481 gcgggtttct gtactgatat tcatgacatg tgctccccgt ccagcgcctg taacacaacg
      541 tccattgctt cgcgttcttc cctgacgaat gcgtcaccgt cgcccctgtt ctcactcccg
      601 acggcataca ggcgcttcat ttcactgcct gccagcgcca tcactttttc cagcatcacc
      661 gccggaactg tcacctgacg gctggcgcgc ttttcttcca tcagctcacg aacaacgtcg
      721 tgaacaatgc tgacatccgc accatattca aaggcatcgt ccagccgctg catgacggtt
      781 tccagctcat cgtctgacag gttccagtcc tgggctatgt ggcgcacatc cccacgggtc
      841 agcaccacgg caaaaacgca ttcactgtcc ggcacgtctg tatccagtac atcgcggatg
      901 atttctttaa cagtacagta catgttctcg tctcctttac aggggcggca ttgccgcccc
      961 cgtccgttaa tcagtccagt gccgcataaa tcgcgttgca ttcggggtga aattcgatgt
     1021 gacgccacaa ctgcgcatca cgcatccggt acaggtgtgt cagtcccgca tcaccgctgt
     1081 cgtggtacag ccacaactgg cggttaatca caagggaagt cagaatgatg cctgcggcat
     1141 cggcgctgac cgtgcggtca aaccagtttg caccgttaac cagatgaaaa cggtcaccgt
     1201 ccggcatcat gtaaccgcca ccgtccggca ggcggatgaa gtgccagaag ccgccttcat
     1261 attccggcat catttgtttc gccagcgcgt acacagacgc ttcggcatac agaaaatcgt
     1321 tgccaaacag gtaaggcaga aaagacagac ggtcgcactc ttcaacattc agagtgacag
     1381 gtttcgcata ctgcatgatg ttttcctccg ttgttcacat attctgcgac accgggcggt
     1441 gccgtgcctc cataaaagcc gggcgataag gcggggatgc aggggcgcaa gggaagccgc
     1501 agcctgacgc actcggctga cgggagttgg agggtgaaac cgcgaaatct gagcgggtga
     1561 acgaccggaa aatcacggca gacgggaagg agggaccggg tgaccgcagc ggcgaagtgt
     1621 acccttgcat caacgccagc ccggctaaga tggaggcacg gcaccgcctg cacacgacgc
     1681 tgaggccaca taactgaaaa cccacaaggc catgccctgc atggccgggt aaagcggagc
     1741 tggcggcgat ttcctttgtg ctctgtccgg ggaccgccat cgtcatcatt tccggatttt
     1801 tgccagggca tccatcatgc tgcggatttc atcccgtgcc tccaggacaa ctcccgcaga
     1861 atattgaggt caaacaagcg gcggggcggt taacccgata ttcacagcac atacagacca
     1921 ggccacttac caggatatgc tctacacact cacttacagg cattaatgga atcgtagtct
     1981 cacgcagata atctgatcga caatacgagt gggaccgtgg tcccaaactg attatcagac
     2041 cgacaataca agtgggaccg tggtcccagg ccgataatca gaccgacaat acaagtggga
     2101 ccgtggtccc aggccgataa tcagaccgac aatacaaatg ggaccgtggt cccaggccga
     2161 taatcagacc gacaatacaa atgggaccgt ggtcccagat ataatattca gaccagttag
     2221 ttatgttttc tagcctgtgc taaaggaaat taagtaaaga cagataaatg tagactaaaa
     2281 cgtgccgcat caaagtgccg ttttttcaag ttccttaaga atggcttcaa ttttctctat
     2341 acactcagtt ggaacacgag atctatctag gtttagcacc attttatcgc ccttgtacaa
     2401 cactgtcgct ccaggagcaa actgatgtcg tgagcttaag ctagttcttg aggcagatga
     2461 cgttttaagc acagatgtta aaagagtgat gacttcttca gcttcaaata tcacccctgc
     2521 ttttttctgc tcgtgaaggt tagatgcctg ttgcttaagt aattcttctt tatctgtaaa
     2581 agctctttga agtgcatcac ctgaccgggc agatagttca ccaggatgag aaaaaagagc
     2641 aacaactgat tttggcagtt tagctgtgtt tatacagcgg gtaataatct tacgtgaaat
     2701 attttccgca tcagccagcg cagaaatatt tccagcaaat tcattctgta atcggcttgc
     2761 ataacgctga ccacgttcat aagcacttgt tgggcgataa tcgttaccca atctggacaa
     2821 tgcagccatc tgctcatcat ccagctcacc aaccagaact cgaaaatcac tttctgtaag
     2881 tgcagcagct ttacggcggc ggctcccatc ggcaatttct atgatcccag acactcttcg
     2941 accaaacgct ggtgtctgct gacctgtcag tagaaaagaa gggataagat catccagggc
     3001 gtcttcagtt agcagttcct ggtcacgttc attacctgac catacccgag aggtcttctc
     3061 aacactatct ccccggagca cctcaagggt aaatttcacg tcccgaccac atacaggcaa
     3121 agtaatagca ttgccacgag ccatagctcc tacgcgcgca attaacgaat ccaccatcgg
     3181 tgcagctggt gccgatgacg aagtatcttc aagcggttga gtcttaagcg tatgttttgg
     3241 aattacaggc gcacgcttca ttatttaatc tcccagcgtg gtttaatcaa acgatcgaaa
     3301 atttcattgc agacaggttc ccagatagaa agggcatttc tccaggcacc tgttgaagag
     3361 cgttgatcaa tagcctgttc aaaaacagtt ctcatccgaa tctgaccttt accaacttca
     3421 tccgtttcac gtacaacatt ttttagaacc atgcttcccc aggcgtcccg aatttgctcc
     3481 tccatccacg gggactgaga accattacta ttactgtatt tggtaagcaa aatacgtaca
     3541 tcaggctcga accctttaag atctacgttc ttgagcagat cacgaagcat atcgaaaaac
     3601 tgcagagcgg aagtgtagtc gaacaactca gcaggcgtgg ggacaatcaa cacatcagca
     3661 gcacatacga cattaatcgt gccgataccc aggttaggcg cgctgtcaat gacaatgaca
     3721 tcatagtcat gagcgacggt ttcaatggcc agccggagca tcaggtgtgg atcggtgggc
     3781 aatttacctt catcaaattt gcccattagc tcagtttcaa tgcggtgcag agccaaacag
     3841 gaaggaataa tgtcaagccc aggccagcaa gtaggcttta ttgcataagt gacatcgtcc
     3901 ttttccccaa gatagaaagg aaggagagta tcctctgcat gaatatgaag atctggcacc
     3961 cagccgtgat acatcgatgc tgttccctgg gggtcgttac cttccacgag cagaacacgt
     4021 agtcccttca gagccagatc ctgagcaaga tgaacagaaa cagaggtttt gtaaacgccc
     4081 cctttatgag cagcaactcc aatcaccggc ggaaatacgt cttcagcacg tcgcagtctc
     4141 gtaccaaaca cgtcacgcat atgattaatt tgttcaattg tataaccaac acgttgctca
     4201 acccgccctc gtatttccat atccgggtgc ggtagccggc ctgctttctc ggcatccctg
     4261 atagcctgag aagatactcc aactaaatcc gctgcttcac ctattctcca gcgtcgggtg
     4321 attttcctcg cttccggact gtcatcatta aactgtgcaa tggcgatagc cttcgtcatt
     4381 tcatgaccag cgtttatgca ctggttaagt gtttccataa gccccattct gaacatcctt
     4441 taatcattgc tttgcatttt tttattaaat cttgcattta attgcaaagc aacaataaaa
     4501 ttgcaaagtt gtttaaaata agagcaacaa aacaaaaaat ataagaagaa caaacacctt
     4561 atcgattggc atagggggta gcacaagact gcgccctgct acaccactca gcatgcagct
     4621 ccaaccgggc agttcgagaa gttgaggtta tgcgagtcaa gggaagccta aagccagctc
     4681 gccttgttcc atcgcacctc atatcagatt cttattcatc ggttcacggg gtcattccgt
     4741 gcttccttcc cacgctcggt caccctcacg cagttgcact tcctttcgct cgctgtagtc
     4801 agctcgcggg agggctttca cctccaagat cgcgcccatg ctgggcgcac acatcctaca
     4861 caccctgaaa aacgtggagg atgtctaatt tatattcccc agaacgtcag attaatagcg
     4921 tttttgatgt cattttcccg gtggctgaaa tcagctactt cttctcctat aacggagacc
     4981 ggcacactgg ccatatcggt agtcatcatg cgccagcttt cccccccgac atgcaccacc
     5041 gggtaaagtt cacgggagac cttatctgat aacagacgtg cactggcctg gggaccataa
     5101 tccgtcgtgt cagcgccagt gatataagac agtaattcac catttggatg gtccgctcca
     5161 cccaacatat tgtttccttg aggttctcac accagaaagg acatcagcat gctgagcaga
     5221 gaggactttt acatgacaaa gcaaatgcgc cagcaaggtg cgtatattgt tgatattgcc
     5281 actcaggttg tttgctctga gcggactgtc atacgatacc tgaacctcca gccagaaaaa
     5341 cgcgccacaa aatggccaaa ctcaagctgt tcatgggcta tatcgacatg cgtcaggctg
     5401 agaacgtctg gaacggcgag gtcctcctgg tagaaatcaa agcgatgggt taaatcggtg
     5461 gtcgttccat gttgcgttac tacatccagc ccaaacgtaa gatgcagccg tcgaagaaaa
     5521 cggttcactt cgaaactcaa gcccggttac cagctacagc atgactgggg agaatttgag
     5581 gctgaggtta ccgggcaacg atgcagggtt aacttcgcgg ttaatacact gtggttctca
     5641 cgtcgcttcc atgtcttcgt ggcaccaaag caggatgctg aacataccta tgagtcacta
     5701 atccgcgcct tccgctactt caacggcagc gtgagaaccg tgctggtcga taaccagaag
     5761 gctgcgttgc tgaaaaataa caacgggaag gtggtgttaa actccgggtt cctgctgctg
     5821 gacgatcact atggcttcct gccacgggca tgccgtccac gaagggccag aaccaaagtt
     5881 aaggtggaac ggatggtgaa atacctcaag gagaacttct tcgtccggta ccgccgattc
     5941 gacagtttcg cccatgttaa tcagcttcac ttcatgttaa gtgattttat gacagttggt
     6001 tggaaaccat ccggattggg ctgctgtcag gtcttctccc ggacccgacc cgcgactttt
     6061 cccgatactg gaacaccatc agaagtattg tcaaacctgc ctatctggcc agccctcccg
     6121 tatttccaga gcatggtatg atggacagcc tgtttgattt tcggatgcgc cagaatacgc
     6181 atactgtcag ggctgggaaa caatgctctc gattatttac tgaaaaaagg tgatatcact
     6241 gacgcagaat atcgggcggc tctggaaatc gatcccctgc aatcgatttc tgtacacctg
     6301 ccgtattcac agacctacct ctgataaaaa gggccagtaa cgcccctttt ctttttcata
     6361 atgttctgat caatcttgcc gggtttcctg cataaatgcc ttttttctca atgtttttta
     6421 ccacaacagc ccccgcacca ataacagctc cgctgcatat atacggagac agaataatgg
     6481 ctccactgcc aacagtaacc gaatccccta ggataatgct gatccagtta tccggtgacg
     6541 gatctggtgc tcctgagtaa acagatcgtt ggcgaacgtc acgttatgac caatgaagca
     6601 gttctcaccc agtgttacgt tctcacaaat aaacgtgtgc gactgtatcc ggcttccacg
     6661 accaatgaca cagcctttct gtatctcaac gaagggtccg acaaacacgt tgtccctgag
     6721 ttcacattta taaatgttga ccggtttaac aatacgtaca ttggttccat aaatcacatc
     6781 ccggacagta cattccataa atttcatact cacacgtcct ttaaatacag atgacacttg
     6841 ccggaattat accgcccaga ttcactctca atatttcaga atagcttata agccgcactt
     6901 ttaccgatgc ccagcaaaaa atcagctctg gctccacttc caccagaaat ttgatctgcg
     6961 cgccgggaaa cacgcagttg gtagtggaac tgacggacgc cggccgggcc gtggcacagc
     7021 ctctgctgct ggcagaaaag gaccgcctcc gggcagaaca gcgtgcggca gaagtcgtcg
     7081 tactgccgct ggtcccggca gcatgtctgc ccgcagacgg cacttctgcc actgatctgg
     7141 cggtagagct gaacggcata acgtatcagg cctgccgtgg ggattttgtg gtgcgtctcg
     7201 acggcagcac ctgtctgcag ttatggaata aagaaggcag agtggtacgc cgggaggacg
     7261 atccgctgga agtggcgcag tggctgcagg catgtcacga tgcaggaatg gaggttcgcg
     7321 tacagataaa tgaaagtgcc gctccgtaag aacacgcctg tgcacggttt acacatcatc
     7381 ccatggtggc agccggttaa ctttcctgcg ccaccagtgg aaagaggcca tgtacagtcc
     7441 ggacaaaaaa ccacccgtga tacttataat aatggcctca cctgccacca ttcctgacgg
     7501 cccccagtaa ataaaccaca ttgcacatcc ccaggaaatg ccccataagc cccccgtcag
     7561 caccgtgacc tgccagaacg gcataaa
//
LOCUS       DADSZD010000184         7529 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.186, whole genome shotgun sequence.
ACCESSION   DADSZD010000184 DADSZD010000000
VERSION     DADSZD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.186"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..544)
                     /locus_tag="J1Z28_003832"
     CDS             complement(<1..544)
                     /locus_tag="J1Z28_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HBA7718619.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIP"
     gene            complement(638..829)
                     /locus_tag="J1Z28_003833"
     CDS             complement(638..829)
                     /locus_tag="J1Z28_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBA7718620.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(826..1014)
                     /gene="dicB"
                     /locus_tag="J1Z28_003834"
                     /gene_synonym="ftsT"
     CDS             complement(826..1014)
                     /gene="dicB"
                     /locus_tag="J1Z28_003834"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HBA7718621.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            complement(1501..2076)
                     /locus_tag="J1Z28_003835"
     CDS             complement(1501..2076)
                     /locus_tag="J1Z28_003835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718622.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALRIRMCSHNDT
                     EHKYEKQNQTAENFVLEPGETLFKIPVTCPICGFTSEELDDSCNNQETTKYVEDDTEC
                     ARRTIISTSPNSRTNKSHFERVINPLPQTNKKDAGGQKTQWNNGISVKKRRSIKDKQR
                     SANNCKRFIPVINFHVLKRIYWWLRHCLMNP"
     gene            complement(2078..2233)
                     /locus_tag="J1Z28_003836"
     CDS             complement(2078..2233)
                     /locus_tag="J1Z28_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HBA7718623.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(2425..2832)
                     /locus_tag="J1Z28_003837"
     CDS             complement(2425..2832)
                     /locus_tag="J1Z28_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002443616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7718624.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            2910..3137
                     /locus_tag="J1Z28_003838"
     CDS             2910..3137
                     /locus_tag="J1Z28_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7718625.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            3121..3642
                     /locus_tag="J1Z28_003839"
     CDS             3121..3642
                     /locus_tag="J1Z28_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718626.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGTVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            3623..4588
                     /locus_tag="J1Z28_003840"
     CDS             3623..4588
                     /locus_tag="J1Z28_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718627.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKKLGLLRCEKLNKSKRDMTNFYTINY
                     ESELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRSNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTAWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            4629..5051
                     /locus_tag="J1Z28_003841"
     CDS             4629..5051
                     /locus_tag="J1Z28_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBA7718628.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRVLTFYQGNYREVML"
     gene            5048..5281
                     /locus_tag="J1Z28_003842"
     CDS             5048..5281
                     /locus_tag="J1Z28_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBA7718629.1"
                     /translation="MSTSATILDMCCGSRMLWFDKNDEWSIFSDIRKEEHSLCDGRHP
                     IINPDIIADFRALPWLSALHQKQTLPDLDICFR"
     gene            5335..6000
                     /locus_tag="J1Z28_003843"
     CDS             5335..6000
                     /locus_tag="J1Z28_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBA7718630.1"
                     /translation="MTVADFKRPKLELPNGAKKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAKQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAYYPGMVYWD
                     YNWRKQGGSSRMIEISKREQFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     GQE"
     gene            complement(6393..6514)
                     /locus_tag="J1Z28_003844"
                     /pseudo
     CDS             complement(6393..6514)
                     /locus_tag="J1Z28_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303812.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6559..6771
                     /locus_tag="J1Z28_003845"
     CDS             6559..6771
                     /locus_tag="J1Z28_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA7718631.1"
                     /translation="MLDTCRLASYVPKGKEKQAMKQQKAMLIALIVICLTVIVTALVT
                     RKDLCEVRIRTGQTEVAVFTAYEPEE"
     gene            6988..7239
                     /locus_tag="J1Z28_003846"
     CDS             6988..7239
                     /locus_tag="J1Z28_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718632.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
BASE COUNT         2018 a         1620 c         1853 g         2038 t
ORIGIN      
        1 caggaataca tttatgctta acccacccaa tggcgtgtaa cttaagctcc ggatacatgg
       61 cgttaacttc tggcattttc atcaacgctt caacgatatg tccgtcgaat gttgccatgt
      121 cttcctgcaa caattcctgt gcgctaataa ccatatcaac ggtgatgttt tcacatgtgt
      181 cgaacttaac catgacagcg ttctgtactt caggggccag cttgtcaaaa gtgacgttca
      241 tcggatcgga ttcagtctca accgggacaa aagaagcaga ctcctcatcc cagcggtttt
      301 cctgcatata ttcagcatcc catgaatcga gggcagggcg gggtatgcca ggtttatcct
      361 cgcagacaat aaatttataa gcgcagtcct gagcagccgg ataatgttcc aggaattgcc
      421 agtgaaattt tgctcgagca cggcgttcgt cgccagcttc aatggctgtg gctacagcca
      481 cagcgccttc ttcccttgtt gccagttcgt caggaatagc ggcgcaaata aagactttac
      541 tcattttgtt ttaacctcat gacagattta aggatgaaca aatccctgcc attgctggca
      601 tataagaatg aaaccggata tttattacgg aactgtttta aagacctgcc gggatttcga
      661 tattatcctg gtgaataact ttatcgaccg ggtaacagtt accgggaatt ttctgttcgg
      721 ttgctgcagt catacactcc tgcattgtcc tgtgaacact gactgcaata tcaactggca
      781 ctccggaaac aagaaaaact gtcagaacaa gcgcaaatgc tgaattcatt gtgcacatcc
      841 ttttggcatc agacgtaaac gagccagcat tgaaacaatg catattttat ttaatagctc
      901 ccgttcttgt tttctcttgt taatggcatc ttcagtaaat acagggttac tgatagtgac
      961 accaatttca aaacaacctt cagacgtatt aacgtttggt aataacgttt tcattatcgc
     1021 gtcctcaaca atgaattttg tgatgcagtg cctggtgcct ccaggtgacg ttaaccagtt
     1081 aacaattaac gtcggataca gagaatccac ccataacact gtttttggtt ttaactgttc
     1141 cgcgtgcgct tagccgcatt caccgcatca caaaattcac tttaaaaacg gcggcagagc
     1201 agtcacggag taaaactgat accgccaaac gtcaccagaa aattgataac agagggcgtt
     1261 gcagcggggt tgtcacttaa gcgtatggtc aacctgacaa ctcggtgtcc tcaacgggga
     1321 aggaataacc ccgccatact taccgccgcg ccattttgcg ggttgccaca accggaagcg
     1381 cacggtcgaa ttaaatttaa cgacaccgta cagtgagacg aacttcgccg tgcgctttcg
     1441 tgttgtgtgc ctgcttttaa ccacgtcagg cgaggtggta tccttaaaat caccacagtt
     1501 ttaaggattc attaagcaat gtcgcaacca ccaataaatc cgcttaagaa catgaaaatt
     1561 gattactggt ataaagcgct tacagttgtt ggcgctgcgt tgtttgtctt taatggaacg
     1621 tctttttttg acagatatcc cgttgttcca ttgggttttt tgtcctccgg catctttttt
     1681 attggtttgg gggagtggat taatcaccct ctcaaagtga gatttattgg tcctggagtt
     1741 tggactcgtg gatataatag ttcttcgtgc gcactcggta tcatcttcga catacttggt
     1801 tgtttcctga ttgttacagg agtcgtcaag ttcttctgat gtaaaaccgc aaatggggca
     1861 cgtaacggga attttgaaaa gcgtttctcc gggttccaga acaaaatttt ctgcggtctg
     1921 attttgcttc tcatatttgt gctccgtgtc attgtgagag cacattctta ttctgagtgc
     1981 ctgtttaaac tcactgaagc tgagagcttc ttcgccttcg gcaaggcctt cgaagtattc
     2041 ttcgtaagcc ttttccatga ttgtgtcgaa atccatatca ctcacctgag tttctttcca
     2101 gccagcgacg ggcaccattt tcggttttaa acgttttgct tttggtatac gtcattgcgg
     2161 tgaacgtgcc gtcctggttt ggaaacacgc cgtacaccag agattcgttg ttgccaagat
     2221 cgatagtatc catgctgacc tcatttcccc ttaacgccgg ggtagcggaa ctgtttgctg
     2281 agaacaccgt gcggtgtctt gatggaggta atttagtttt ctcattattt ttcgtcaagg
     2341 tttttgatga gaaaactcaa gtattgacgc gagataaagc caatacattg aaatgtaagg
     2401 ctttaaaatt ttgtgaaggg atgattattg atgcttgttg cgtttgcgag cctctaatag
     2461 ctcggtgaac aggcgattaa aattctcaac gcgggcgcgg agttcgctga gttgtgcttg
     2521 ttgctctgat ttaggaagtg cacgatacaa acgcagcatc tctaactcat cttccgataa
     2581 gtctaaggca ctatcgagtg aaacaggggg agctggtgtt ttgtcctcgt cgccaaacag
     2641 tatccaggtt ggtgaacatt gcaatacctc ggcgaggcga tgcaaatttt gcccgcgcgg
     2701 ggctgtatgg tcactttccc ataatgaaat tgatgagcca gatacgccag cagctttgct
     2761 taaaccgttt tgacttaagc ctacctgctt acgtctttct ctaatgcgtt gacctaaagt
     2821 tttctcgttc atatttagat atcttaataa cccttgactt gagattcctt gagtgattag
     2881 cattgagaaa actcaatatt ggaggtgcga tgtttaaatc agacgtaatt aatttttacg
     2941 gtacgaaagc caaagtagcg aaagctgctg gcgttgaccc atctgctgtt tctcaatggc
     3001 aagagctggt tcctgaaggt cgcgcgatgc gtctacagga ggcatctggc ggcgagcttc
     3061 agtatgatcc caaggtttat gacgaatatc gtaagacgaa gcgggcgggg cggttgaaca
     3121 atgaaaatca ctcctgaaca ggctcgtgag gctctagatg cctggatatg tcgaccagga
     3181 atgacacagg agcaggcgac gatattaatc actgaagcat tctgggcttt gaaagagcgc
     3241 ccgaacatcg atgttcagcg tgtcacagat gaaggtggca cggttgatca gcgagcgctt
     3301 ggcgttaatc gagtgaagat attcgaacgc tggaaggcta tcgacaccag ggataagcgt
     3361 gaaaagttca cggcgttagt gcctgcaatt atggaggcta tccggattaa tgatttcagg
     3421 ttgtatcgtg aaattagtga cggaaaaagc atcacgtata tgatcgccgg gttaaacaaa
     3481 gaatatggcg atgtggtgga gtccggactg ctttttgctg atcctgccgt agtggatcgt
     3541 gaaactgacg aacttataga aaaagcaatt gctttcaaac ttgcgtatcg acagcaatac
     3601 caacaaaaag ctggatggaa ttatgagcct tctttttgct gaacgcccac tggttataaa
     3661 cacacagctt gcgatgaaga ttggcttaaa tgaagccatt gtgttgcagc agttgcatta
     3721 ctggttgaga gataccaatt ccggcatgga atgtgatggt gttcgctgga tttacaacac
     3781 aacggaacaa tggctggaac agttcccatt ctggtcagag tcaacgttaa agcgcgcgtt
     3841 tgcaagtctg aaaaagctgg ggcttttgcg ttgtgaaaag ctcaataaat caaagcgcga
     3901 tatgaccaat ttctacacga tcaactacga gagcgagctt ttagatgatg gcaaattgag
     3961 cgaatccatc ggttcaaaat gcgccgctcc atcaggtcaa aatgacacga tggaagaggt
     4021 caaaatgaaa cgctccattg gttcaaaacg atccaatgtc atcgggtcaa aatggcctga
     4081 tgatcttaca gagaatacaa cagagattac tacagagaat aaaaacactt ttcgtccgga
     4141 agcttcgcaa ccggacccgc agacgactga acaggatttt ttaacccgga actccgacgc
     4201 ggttgtgttt agtgcgaaaa aacgccagtg gggtagcagg gaggatttgg cgtgtgcgca
     4261 gtggatctgg gggcggatcg tgggtctcta cgaacaggca gccagtgatg atggcgagat
     4321 catgcgacca aaagagccta actggactgc ctgggccaat gacgtgcgca caatgcggat
     4381 gctggatggc agaagccaca gacaaatttg cgaaatgttt ggtcgggttc agcgagatcc
     4441 attctgggta aaaaacatca tgagcccgtc aaagctccgc gaaaaatggg acgaactggt
     4501 catccgcctg ggacgttcac ctgtacagcg ttgtgttaat catatttctg aaccggatac
     4561 agaaattccg cctggtttca gaggataaat gttgatttca ggtcatgagg taattttaag
     4621 ggggacttgt ggcaaaagtt tttacacaag aagagcggga aaaaattaaa gggcaggtgg
     4681 tggaactcgt gcgccagagc ggtcgtgaga cgttacggca actggaagct aaaacaggtg
     4741 cgactagata tctgatgagt gttcttgcca gagagctggt agccagtggc gatgtataca
     4801 actccggcta cgggttattt ccgtctgaac aggcgcgtaa ggactggcaa aacgcccgca
     4861 aaaaactctc gagggcaaag gtgaagaaac ctgctgtggt tgatccggac cttatctggt
     4921 cattacctga tggagaaata cgccgctacg acagtcgtct aaacataatc tgtcgcgagt
     4981 gccggaagag cgaagttatg cagcgtgttc tgacgtttta tcaggggaat tatcgggagg
     5041 tgatgctgtg agtacatcgg ctaccattct cgacatgtgc tgtggcagtc gcatgctctg
     5101 gttcgataag aatgacgaat ggtcgatatt tagcgatatc aggaaagagg aacattcgct
     5161 atgtgatgga cgtcacccga ttattaatcc cgacattatc gctgattttc gtgcgctgcc
     5221 gtggttgtct gctttgcatc aaaagcagac attaccagat ttagacatct gtttccgata
     5281 gcccgttctg atgctacact ttttgctatt ttcatgaacc tcataaacac atttatgact
     5341 gttgctgatt tcaaacgccc caaattggag ctcccaaacg gggcaaaaaa actattactg
     5401 cactcttgct gtgctccatg ttccggtgaa gtgatggagg cgcttcaggc ctcgggaatc
     5461 gactacacca tctttttcta caacccgaac attcatcctc agaaagagta tttaattcgt
     5521 aaggatgaga atattcgctt tgctaaacaa cacggcgtgc catttattga tgctgattac
     5581 gacacagaca actggtttga gcgagccaaa ggaatggaat gggagcctga acgagggatc
     5641 cgttgtacca tgtgttttga catgcgtttt gagcggactg cgctgtatgc cgctgaaaat
     5701 ggtttcagtg tgatcagtag ctcactgggc atttcacgct ggaaaaatat gcagcaggtt
     5761 aacgactgtg gacggcgagc cgtcgcgtat tatccgggca tggtgtactg ggattataac
     5821 tggcgaaagc agggcggctc ttcccgcatg attgaaatca gcaagcgcga acaattctat
     5881 cagcaggaat attgtggctg cgtgtattca ctgcgcgata ccaatctaca ccgcaaatct
     5941 cagggacgcc ctctaatcaa aattggtcaa ctccactacg gtaaagaagg gcaggaatga
     6001 ttttatatta tgccaacaac ggagcgtagc agtgtttcta tatatagtac gcctacctta
     6061 attttcaaat aatcatgatt aatgagcagg tcatcagtgc aagcaatgac ctgctcatct
     6121 tgttgaaatt agaggaaatc ttgaagccat gatatggctt aaaataacgt ttgattattt
     6181 tacctgctgt ttgtccctgg tttaaagtcg gttgtctgat ttgattacgc gcaactgacg
     6241 cttacgtatt gaagtaacga gcacgaagac acatatttat cagtgcctgt atacgtcaag
     6301 agcaggagat ttatgtgctg gttctcaaat gcacgctggc tattgtggct gtaatggcaa
     6361 tttattgtct tgttattgtt cttatggatc accttactga ttgatttcat attggcgagg
     6421 taacggtagt taagtagaat ggctgcgggt gcttgaggct atctgcctcg ggcatgaaca
     6481 ccaacggcag atagagaaaa gccccagtta acattacgcg tcctgcaaga cgcttaacat
     6541 taatctgagg ccaatttcat gctagacaca tgtaggttag cctcttacgt gccgaaaggc
     6601 aaggagaagc aggctatgaa gcagcaaaag gcgatgttaa tcgccctgat cgtcatttgt
     6661 ttaaccgtca tagtgacggc actggtaacg aggaaagacc tctgcgaggt acgaatccga
     6721 accggccaga cggaggtcgc tgtcttcaca gcttacgaac ctgaggagta agagacccgg
     6781 cgagggagaa atccctcgcc acctctgatg tggcaggcat cctcaacgca cccgcactta
     6841 acccgcttcg gcgggttttt gtttttattt tcaacgcgtt tgaagttctg gacgatgccg
     6901 gaatagaatc aaaaatactt aagtagcgcg cagggataag agggatggtc ccttaaaggg
     6961 gagagctaat tatccggaag gattctgatg atgaacatcg aagaactgcg taaaattttt
     7021 tgtgaagatg gcctctatgc tgtgtgcgtt gaaaatggaa atcttgttag tcattaccgc
     7081 attatgtgtt tgcgaaagaa tggggctgcg ttaattaatt ttgtggatgg tcgagtgaca
     7141 gacggattta tcttgcgcga aggtgagttt gtcacttcat tacaggcact gaaagagatc
     7201 ggaataaaag caggcttttc agcttttgca gaagaataaa ctcatctaca atcttgcgcg
     7261 gggctgaact cccgctgagt aacaccgtgc caccggagaa aaccgatggc acgcaacgta
     7321 aaatattaca attctgataa ttcgcccgtt cttgcctgca cgcacgagcg gtattctcac
     7381 gcattcaagt ctgaatggtt ccagcaccct ccatgcactg aagagcaggc tgaatggata
     7441 attcagtgtt accgcaggcg cggatacgag gttaagaaag cccttagtct cgactaccgt
     7501 cactggataa tctcagtcag attgcctta
//
LOCUS       DADSZD010000185         7515 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.187, whole genome shotgun sequence.
ACCESSION   DADSZD010000185 DADSZD010000000
VERSION     DADSZD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.187"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(54..1121)
                     /gene="purK"
                     /locus_tag="J1Z28_003847"
     CDS             complement(54..1121)
                     /gene="purK"
                     /locus_tag="J1Z28_003847"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBA7718633.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(1118..1627)
                     /gene="purE"
                     /locus_tag="J1Z28_003848"
     CDS             complement(1118..1627)
                     /gene="purE"
                     /locus_tag="J1Z28_003848"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBA7718634.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(1723..2052)
                     /locus_tag="J1Z28_003849"
     CDS             complement(1723..2052)
                     /locus_tag="J1Z28_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718635.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            complement(2163..2885)
                     /gene="lpxH"
                     /locus_tag="J1Z28_003850"
     CDS             complement(2163..2885)
                     /gene="lpxH"
                     /locus_tag="J1Z28_003850"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBA7718636.1"
                     /translation="MATLFIADLHLCAEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LKLYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQTAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(2888..3382)
                     /gene="ppiB"
                     /locus_tag="J1Z28_003851"
     CDS             complement(2888..3382)
                     /gene="ppiB"
                     /locus_tag="J1Z28_003851"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBA7718637.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3556..4941
                     /gene="cysS"
                     /locus_tag="J1Z28_003852"
     CDS             3556..4941
                     /gene="cysS"
                     /locus_tag="J1Z28_003852"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBA7718638.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(4977..5498)
                     /locus_tag="J1Z28_003853"
     CDS             complement(4977..5498)
                     /locus_tag="J1Z28_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBA7718639.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKAIPPRLLFAGIVLAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(5606..5818)
                     /gene="ybcJ"
                     /locus_tag="J1Z28_003854"
     CDS             complement(5606..5818)
                     /gene="ybcJ"
                     /locus_tag="J1Z28_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBA7718640.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(5820..6686)
                     /gene="folD"
                     /locus_tag="J1Z28_003855"
     CDS             complement(5820..6686)
                     /gene="folD"
                     /locus_tag="J1Z28_003855"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBA7718641.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            6727..6924
                     /locus_tag="J1Z28_003856"
     CDS             6727..6924
                     /locus_tag="J1Z28_003856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718642.1"
                     /translation="MSILSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYIR
                     CGRTLVQQLVQKPLTQQGLTV"
     gene            6957..7033
                     /locus_tag="J1Z28_003857"
     tRNA            6957..7033
                     /locus_tag="J1Z28_003857"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6991..6993,aa:Arg,seq:tct)
     gene            complement(7049..7515)
                     /locus_tag="J1Z28_003858"
                     /pseudo
     CDS             complement(7049..7515)
                     /locus_tag="J1Z28_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
BASE COUNT         1805 a         2003 c         1964 g         1743 t
ORIGIN      
        1 cggtaggcct gataaggcgt tcacgccgca tgcggcaaga aaatgagcat caattaactg
       61 aacttgctct gcgcccacat cacgccgctg gcatactccg gcggcagcag tgggattaag
      121 gcttccagcg tcgcggtcag acgcgatgtg tcgctgtcgg tcaaattcaa atgccccact
      181 ttacggcccg gacggacttc tttgtcgtac cagtgcagat gcacaagcgg cagtttcagc
      241 cagtcataat tcagatcgct accaatcaga ttgatcatca ccgacggatt attcactact
      301 ggttgcggta acggcagatc ggtaattgcc cgcagatgta gctcaaactg gctgatgctg
      361 gcaccgtttt gcgtccagtg accgctgtta tgcacacgcg gagccagttc gttgatcagc
      421 agaccttgtg gggtgacaaa acactccatt gccatcacgc ctacatagct cagctcctgc
      481 ataatcgccg acaacatctc ttcagcttgc gcctgcagct gtgcgttggc ctgcgggaaa
      541 gcgacgctgg tgcgcaaaat gccgtcctga tgcaggttat gcgtcagcgg ataaaacacg
      601 gtgctgccat caaaaccgcg cgcgccaacc agcgacactt caccagagaa gtttatgccc
      661 tgctcgacaa tacattcgcc gtagcattca gcaggtaact gttcggtttc atcggcgcgt
      721 aaacgccatt gaccgcgtcc gtcatagcca ccagtgcggc gcttaacaat cgccagctca
      781 cctaaacgat caaacaccgc aggccactcg ctgcgttcgg aaagtaactg ccacggtgcg
      841 gtcggcaggt ggagcttatc gaaaagctgc ttctgagtca gacggtcggc aataatcggg
      901 aacacatcgc ggttcacaaa ggccggatgg cgcgccagct cgcgggttaa tgcagtttcc
      961 ggccagcgtt cgatctcagc ggtaatcacg ctttgttgaa aaggcaccgc cgccggttca
     1021 gcgtccagcc cgaccggcca gacagcaatg cctaacggtt cgcctgcctg acgcagcata
     1081 cggcctaact gcccgttacc gaggacgcaa acctgtttca tgccgcacct cgcgggtccg
     1141 ggttttccag cacttcatca gtttgtgctt tgcgccattc attcagacgc tggtgcagtt
     1201 ctttatcatg agtcgcaaga atttgtgctg ccagtaacgc cgcgtttgcc gcgccagctt
     1261 taccaatcgc cagcgtaccc accggaatgc cgcgcggcat ttgtacgatg gagtagaggc
     1321 tatcgacacc gctcagtgcg gcgctctgta ctggcacgcc cagcaccggc accagcgttt
     1381 tggcggcaat catgccaggc aggtgcgctg cgccgcctgc gcccgcaata atcacctgat
     1441 aaccgttctc ttcggcgctt tcggcgaagc tgaacagttt atcgggggtg cggtgagcag
     1501 aaaccacttc aacgtggtgc gggacattca ggatttcgaa gatttcggcg gcgaactgca
     1561 tggtagccca gtcgcttttg gaccccatca cgatggcgac acgcgccgga ttattgcggg
     1621 aagacatgcg tcttaaaact cctgtggtgc acaactctcg gctttagagg gcacagagaa
     1681 tagcacggaa agagtgcaag gaaaacggtt gcgtaggcgg aattaaccgt cagttgttga
     1741 gtttttaatt tgttctgcgt gttgattatg cgctctggct gaactggcaa agaatgccca
     1801 tatcaacaca atgctccatg gaaaaaagca aataccgaca atcaatataa atctttctac
     1861 aaaacgttgg ttatgggggc gggtgagtac taaataaatg agccagaaat agactatcca
     1921 ggaaatgacc gccgcagcta ccagtgacaa tgcgccaaaa tagaagccga caagaccgaa
     1981 ccagctaata cacagtggcg ctcctgccgt aagggcgatg ccagaaatca aaaatccgtt
     2041 gcagcgttgc ataataatat tccctgttgt tatttcactc agagattatt accttgctga
     2101 ttttaaaaaa gatatacagg ttgcgttagt tcggtgcttt ttcagaatct gtccattcgt
     2161 ggtcagaaag gaaaatggat tagctcaacg tcatccgccg tgactttcac catcgaacct
     2221 tctgtatgcc aggcacccag taccacgcga aaagccgttt gctgattggc gataagttca
     2281 tgcaccgccg ggcgatgggt atgcccgtgg atcagccatt gcacttgatg tttttccatc
     2341 gcactgacca ccgcgttttg gttaacgtcc atgatcgcca gcgatttact gctgttggct
     2401 tctttactgt tcgcgcgcat tcgcgcggca atgcgtttgc gcacaaacaa cggcagggcg
     2461 aggaatagcg tttgcagcca gggtttgtgg actttggcgc gaaaagcctg ataacctgcg
     2521 tcatcggtgc acagcgtgtc gccatgcata atcaacaccc gacgaccata aagtttgagc
     2581 accttttctt ccggcaataa cgtcatgccg ctttcacggg caaagcgttt gccgagcaga
     2641 aaatcacggt tgccatgaat gaaataacag ggaacgccgg aatcggatac cgctttgatc
     2701 gctgcggcca tctggtgatg aagtgggttg ggatcgtcgt cgccgatcca ggcttcaaac
     2761 agatcgccaa gaatatacag cgcatcggcc ttgcgggcct cccccgctaa aaaacgcaga
     2821 aaaccggcgg tgatcgccgg ttcttccgcg cagagatgaa gatctgcaat aaagagtgtc
     2881 gccacgatta ctcgctaacg gtcacgcttt caatgataac gtcttctttt ggcacgtcct
     2941 ggtgcatacc gctacgaccg gttgccacac ctttgatttt gtctaccacg tccatgcctt
     3001 caaccacttc agcaaatacg cagtagcccc aaccttgcag gctttcgcca gagaagttca
     3061 ggaagtcgtt atcaaccacg ttgatgaaga actgggcagt tgcagagtgc ggtgcctgag
     3121 tacgggccat tgccagcgta ccacgggtat ttttcagacc gttgttggct tcgtttttga
     3181 tcggttcttt ggtggctttt tgtttcatgc ccggttcaaa accgccgccc tgaatcataa
     3241 agccgttgat aacacggtgg aaaatggtgt tgttgtaaaa accttcgcgg cagtagtcca
     3301 ggaagttttt aactgtttca ggtgctttat cgtcaaaagt tttgatgaca atatcgccgt
     3361 gattggtgtg gaaagtaacc atttttgcat cctgttccgt ttgattggtg cttcaacccg
     3421 gttcgggtca tatatagggt ggtgttatag cataaccgca cgatcggatc atctcgcaat
     3481 gtatgctgat tcgcgcggga aatatgggta ttatacgcaa ctcaattacc cacacatgtc
     3541 taaacggaat cttcgatgct aaaaatcttc aatactctga cacgccaaaa agaggaattt
     3601 aagcctattc acgccgggga agtcggcatg tacgtgtgtg gaatcaccgt ttacgatctc
     3661 tgtcatatcg gtcacgggcg tacttttgtt gcttttgacg tggttgcgcg ctatctgcgt
     3721 ttcctcggct ataagctgaa gtatgtgcgc aacattaccg atatcgacga caaaatcatc
     3781 aaacgcgcca atgaaaatgg cgaaagcttt gtggcgctgg tggatcgcat gatcgccgaa
     3841 atgcacaaag attttgatgc tttgaacatt ttgcgcccgg atatggagcc gcgcgcgacg
     3901 caccatatcg ccgaaattat tgaactcact gaacaactga tcgccaaagg tcacgcttat
     3961 gtggcggaca acggcgacgt gatgttcgac gtcccgaccg atccgacata tggcgtgctg
     4021 tcgcgtcagg atctcgatca gctgcaggca ggcgcgcgcg ttgacgtggt cgacgacaaa
     4081 cgcaacccga tggacttcgt tctgtggaag atgtcgaaag agggcgaacc gagctggccg
     4141 tctccgtggg gcgcgggccg tcctggctgg cacattgagt gttcggcaat gaactgcaaa
     4201 cagttgggta accactttga tatccacggc ggcggttctg acctgatgtt cccgcaccac
     4261 gagaacgaaa ttgcccagtc cacctgtgcg cacgatggcc agtatgtgaa ttactggatg
     4321 cactccggta tggtgatggt tgaccgcgag aagatgtcta aatcgctggg taacttcttc
     4381 accgtgcgtg atgtgctgaa atactacgat gcggaaactg tgcgttactt cctgatgtcg
     4441 ggtcactatc gcagtcagtt gaactacagc gaagagaacc tgaagcaggc gcgtgcggcg
     4501 ctggaacgtc tctatactgc cttgcgcggc acagacaaaa ccgttgcgcc tgccggtggc
     4561 gaagcgtttg aagcgcgctt tattgaagca atggacgacg atttcaacac cccggaagcc
     4621 tattccgtgc tgtttgatat ggcgcgtgaa gtgaaccgtc tgaaagcaga agatatggca
     4681 gcggcgaatg caatggcgtc tcacctgcgt aaactttctg ccgtattggg cctgctggag
     4741 caagaaccgg aagcgttcct gcaaagcggt gcgcaggcag acgacagcga agtggctgag
     4801 attgaagcgt taattcaaca gcgtctggat gcccgtaaag cgaaagactg ggcggcggca
     4861 gatgcggcgc gtgaccgcct taacgagatg gggatcgtgc tggaagatgg cccgcaaggg
     4921 accacctggc gtcgtaagta attgcgctat tgccggatgc gagttttcgc atccggttat
     4981 cgtctgcgcc accacaacat tcccatcagt agcatccccg gcaaccacac ccacatcaat
     5041 tcagaaataa tcacctgatg cccgtacggc gtggtgtaac gagacaatgc aaacggcgcg
     5101 actttaatca cctgccaggg agcgaaaaag cgttcatctg accacggcca cagccagcca
     5161 acccctttac cgccagtggt taccgaatcc agcaagctgt gcgatagcaa cgagacggtt
     5221 aaaaacagcc agcagcgaat cagcccagcc ctgaaccatc gacgtccaat cagcacacat
     5281 aacagcggga caacaaacgc aaacaccagc gaatgggtaa acccgcgatg accaaaaacg
     5341 ttgccgtaag caacgccaaa tttaaacgat aatacgtcgg catcaggcaa catcgccagg
     5401 acgattccgg caaataacag acgcggtggg atggctttcg aacccaaccc taaaccaatg
     5461 cataggggaa cggcggcgtg cgtaataacg gttggcatga tggtcgcttc ggcaaaatgt
     5521 cgatgctatc agcatggatg aacggggcgt agagggcaaa agtctgaaaa gagaaccggc
     5581 ctgttgatac aggccgggaa agggatcagg caacaacctg tacgctgtga cctgcaaaac
     5641 tcactgtctg accggcgaca attttgcagc gtttgcgcgt ttcaaccgca ccgtcgactt
     5701 tcacctggcc ttcggcaatc gcgattttcg cctgcgcgcc gctttcgctc cagccttcca
     5761 gtttcagcaa gtcgcacagc tcaacgtgcg gatgtttacc taaagaaaat gtcgccatgt
     5821 tactcaccct gtggatcatg atattcaacg cacgcctgta gcgtgttttc aatcagcgtg
     5881 gcaaccgtca tcgggccaac gccgcccgga acaggcgtaa tgtatgaggc gcgtttagcc
     5941 gcgtcttcaa acacgacgtc gcccacaact ttgccatttt ccagacggtt gatgccgaca
     6001 tcaatcacaa ttgcgccttc tttgatccag tcaccaggaa taaagcccgg cttgccgacg
     6061 gcaacgatca gtagatcggc attttctacg tggtgacgca gatttttagt gaaacggtgg
     6121 gtcacggtag tggtgcaacc tgccagcagc agttccatgc tcattgggcg accgacgata
     6181 ttcgatgcgc caatcaccac ggcgttaagg ccgaaggtat caatgttgta acgctcaagc
     6241 agcgtgacga taccacgcgg ggtgcaggga cgcagacgcg gcgcgcgctg gcacaggcga
     6301 ccgacgttat aaggatggaa gccgtctacg tctttgtccg ggtgaatacg ttccagtact
     6361 ttgacgttat caatacccgc cggtaacggc agttgaacca gaatgccatc aatggtgttg
     6421 tcggcattca gcgcatcgat aagctccagc agctccgctt cgctggtggt ttccgggagg
     6481 tcataagagc gggagacgaa cccgacttct tcacaagcct tgcgtttgct tgcgacataa
     6541 atttgcgatg cagggttact gcccaccagc acaacggcca gtcctggtgc ccgcagtccg
     6601 gctgcaatac gcgcctgaac tttttgagca acttcagagc gcacctgctg cgcaatcgtt
     6661 ttaccgtcaa taatctttgc tgccatcaga gagaggattc catctgttac gtaggtcgaa
     6721 ggggatgtgt ctattttgtc agaagcgtgg cgcgctgtca gttttcgttt tctgaaaagt
     6781 aaaaccttct gtacctggct gattcacacc tatcatgata accgtcgttg gtttgccgcc
     6841 atagtgagct acatcagatg cggtcgtaca cttgttcagc aactggttca aaagccattg
     6901 actcagcaag ggttgaccgt ataattcacg cgattacagc acaccgtggt atcaatgcgc
     6961 ccttagctca gttggataga gcaacgacct tctaagtcgt gggccgcagg ttcgaatcct
     7021 gcagggcgcg ccattaaaat tcaatcagtt acgccttctt tatttcttct ataattccag
     7081 agtgggacat tatcaccaaa aatgtcgtct attttcctcg cgtgctcagt caaatgatta
     7141 ggcgcaaggt gagcatatct acgaaccatt tctctggact cccatccgcc catttcctga
     7201 agcactgata atgggacgcc tgacggaatc aaccagcttg cccaggtatg tctgaggtca
     7261 tggaaacgga aattttcaat tcctgcacga cgacaagctg atagccatgc tgtcttgctg
     7321 tcaatgcgca tcttcctgag cgcaggcatt gatgttccat ctgctcgctt agccgccctg
     7381 gtatgtacac ccacatttgt gatgcttgcc tgtttgatca cgcaacactt tagaggcggt
     7441 atcgttcagc gccacaccaa tggcgcggtt tgatttgcta tcttctggat tcaccctggc
     7501 aacttgtcgc tgcat
//
LOCUS       DADSZD010000186         7466 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.188, whole genome shotgun sequence.
ACCESSION   DADSZD010000186 DADSZD010000000
VERSION     DADSZD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.188"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..1361)
                     /gene="pgtP"
                     /locus_tag="J1Z28_003859"
     CDS             complement(<1..1361)
                     /gene="pgtP"
                     /locus_tag="J1Z28_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transporter PgtP"
                     /protein_id="HBA7718643.1"
                     /translation="MLSIFKTGQAADSVPAEKIQVTYRRYRMQALLSVFLGYLAYYIV
                     RNNFTLSTPYLKEQLDLSATQIGVLSSCMLIAYGISKGVMSSLADKASPKVFMACGLV
                     LCAIVNVGLGFSTAFWIFAVLVILNGLFQGMGVGPSFITIANWFPRRERGRVGAFWNI
                     SHNVGGGIVAPIVGAAFALLGSEHWQSASYIVPACVAIVFAVIVLILGKGSPRQEGLP
                     SLEEMMPEEKVVLNTRQTVKAPENMSAFQIFCTYVLRNKNAWYVSLVDVFVYMVRFGM
                     ISWLPIYLLTVKHFSKEQMSVAFLFFEWAAIPSTLLAGWLSDKLFKGRRMPLAMICMA
                     LIFICLIGYWKSESLFMVTIFAAIVGCLIYVPQFLASVQTMEIVPSFAVGSAVGLRGF
                     MSYIFGASLGTSLFGIMVDHIGWHGGFYLLGCGIICCIIFCWLSHRGAIELERHRAAY
                     IKE"
     gene            1760..2977
                     /gene="pgtC"
                     /locus_tag="J1Z28_003860"
     CDS             1760..2977
                     /gene="pgtC"
                     /locus_tag="J1Z28_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate transport regulator PgtC"
                     /protein_id="HBA7718644.1"
                     /translation="MILLLLLMDTGAVRAQRNELVMATTFSPGATAWIIQRWQTEPES
                     VMIRTLNRTSASLEQLLYTANVENVDLILTSSPMLLQHLQEHQKLAPFDDAPAESQNL
                     VPESIRATSVAVAISGFGLLINRPALSVKHLPAPADWDDLALPIYQDALLMSSPSRSD
                     TNHLMVESLLQQKGWVKGWETLLTSAGNLVTISSRSFGVADKIKSGLGVAGPVIDNYA
                     NLLLNDPHLSFTYFPRSAVSPTYVAILKKSPHADAARRFIHYLLSPKGQRILADANTG
                     KYPVTPLAADNPRATQQQLLMNQPPLNYHLILKRQSLVQRLFDTAISFRLAQLKDAWR
                     ALHSTEARLKKPLPEIRALLTQVPVAAASSEDPVWLAQFDNKSFTEQQMMKWQLWFLN
                     NQRLAIKKLEELK"
     gene            2974..4983
                     /gene="pgtB"
                     /locus_tag="J1Z28_003861"
     CDS             2974..4983
                     /gene="pgtB"
                     /locus_tag="J1Z28_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004137022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PgtB"
                     /protein_id="HBA7718645.1"
                     /translation="MKNITLWQRLRQVSISTSLRCAFLMGALLTLIVSSVSLYSWHEQ
                     SSQIRYSLDEYFPRIHSAFLIEGNLNLVVDQLNEFLQAPNTTVRLQLRTQIIQHLDTI
                     ERLSRGLSSRERQQLTVILRDSRSLLSELDRALYNMFLLREKVSELSARIDWLHDDFT
                     TELNSLVQDFTWQQGTLLDQIASRQGDTAQYLKRSREVQNEQQQVYTLARIENQIVDD
                     LRDRLNELKSGRDDDTQVETHLRYFENLKKTADENIRMLDDWPGTITLRQTIDELLDM
                     GIVKNKMPDTMREYVAAQKALEDASRTREATLGRFRTLLEAQLGSTHQQMQMFNQRME
                     QIVRVSGGLILVATALALLLAWVFNHYFIRSRLVKRFTLLNQAVVQIGLGGTETTIPV
                     YGNDELGRIAGLLRHTLGQLNVQKQQLEQEITDRKVIEADLRATQDELIQTAKLAVVG
                     QTMTTLAHEINQPLNALSMYLFTARRAIEQTQKEQASMMLGKAEGVISRIDAIIRSLR
                     QFTRRAELETSLHAVDLAQMFSAAWELLAMRHRSLQATLVLPQGTATVSGDEVRTQQV
                     LVNVLANALDVCGQGAVITVNWQMQGKTLNVFIGDNGPGWPEALLPSLLKPFTTSKEV
                     GLGIGLSICVSLMEQMKGELRLASTMTRNACVVLQFRLTDVEDAK"
     gene            4973..6220
                     /gene="pgtA"
                     /locus_tag="J1Z28_003862"
     CDS             4973..6220
                     /gene="pgtA"
                     /locus_tag="J1Z28_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PgtA"
                     /protein_id="HBA7718646.1"
                     /translation="MLSSEYSILLIDDDADVLDAYTQLLEQSGYRVFACNNPFEAQAW
                     IQPDWPGIVLSDVCMPGCSGIDLMMLFHQDDQQLPILLITGHGDVPMAVDAVKKGAWD
                     FLQKPVDPGKLLSLVEEALRQRQSIIARRQYCQQTLQVELIGRSEWINQYRRRLQQLS
                     ETDIAVWLYGAPGTGRMTGARYLHQFGRNAQGEFVYRELTPDNAPQLNDFIALAQGGT
                     LVLSHPEHLTREQQYHLVQLQSQEHRPFRLIGIGDTSLVELAASNHIIAELYYCFAMT
                     QIACLPLTQRPDDIEPLFRHYLCKACQRLNHPVPEVGKEMLKEMMRRMWPNNVRELAN
                     AAELFTVGILPLAETANPLMHVGTPAPLDRRVEDAERQIITEALNIHQGRINEVAEYL
                     QIPRKKLYLRMKKYGLSKEHYKN"
     gene            6600..7466
                     /locus_tag="J1Z28_003863"
                     /pseudo
     CDS             6600..7466
                     /locus_tag="J1Z28_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174402.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         2084 a         1796 c         1800 g         1786 t
ORIGIN      
        1 tgttctttta tatatgcggc tctgtgacgt tcaagttcaa ttgcaccacg atgtgataac
       61 cagcaaaaaa tgatgcaaca aataataccg cagccaagaa gataaaatcc gccatgccag
      121 ccaatatgat cgaccataat accaaacagg ctggtgccca gagatgcacc gaagatatag
      181 ctcataaaac cacgtaaacc taccgcggag ccaacagcaa agctgggcac gatctccata
      241 gtctgaacag aagccagaaa ttgtggaacg taaatcagac aaccaacaat ggcagcaaaa
      301 attgtcacca taaacagtga ttcacttttc cagtagccaa tcaggcaaat gaaaatcagc
      361 gccatacaaa tcatcgccaa tggcatacga cgccctttaa acagtttgtc tgacaaccaa
      421 ccggcaagta gcgtggaagg gattgcggcc cattcaaaaa ataaaaacgc gacgctcatt
      481 tgttctttag aaaaatgttt caccgtcagc agataaatag gcaaccagct aatcatcccg
      541 aagcgtacca tgtatacaaa tacgtcaacc agtgagacat accaagcatt tttattgcgt
      601 aatacataag tgcagaaaat ctgaaaggca ctcatgtttt ctggtgcttt taccgtctgt
      661 cgggtattca ggacgacttt ttcttccggc atcatctctt ccagagaggg tagaccttcc
      721 tgacgtgggg aacctttacc gagaatcagc acaattaccg caaaaacgat ggccacgcag
      781 gccggaacga tatagctcgc actttgccag tgctcgctgc cgagtagggc aaaagcggca
      841 ccaacaatgg gtgcaacaat accaccaccg acgttatgag agatattcca gaaagcacca
      901 acccgaccac gctcccggcg agggaaccag ttagcaatag tgatgaaaga aggaccaacg
      961 cccattccct ggaaaagacc attcagaata accaataccg caaaaatcca gaatgcggtg
     1021 ctgaatccca ggccaacgtt aacgatggca cataacacca gcccacacgc cataaagact
     1081 ttcggactgg ctttatcggc aaggctactc atcactcctt tgctgatacc ataggcgata
     1141 agcatacagc tactcagtac gccaatttgt gtggcgctga gatctaattg ctctttaaga
     1201 taaggcgtcg ataaggtgaa attattacgc acgatatagt atgcaagata ccccagaaat
     1261 acactaagta acgcctgcat acgatagcga cgatatgtca cctgaatttt ctccgccgga
     1321 acactatccg ccgcttgccc cgttttaaat attgataaca tgtcttcacc tgtattttat
     1381 attgatatga acacctggag tattctgaca atgagagtta tataattgag atgaaacatc
     1441 aaactgaaaa aatgtgtcac aattgacaca aattaataaa atcaaagcat gtttttgacc
     1501 cattttattg atgcatagac agggtagatt tatgctttat ctgttttatt gttagcatgt
     1561 gcggatatta acaaatctca tgctgattat cgggtaatca ctgattaata tattaacgct
     1621 ctctttgagc agatgtgtta tcagacgaag tgtctgaaac gggtaatttt tgtctcaatg
     1681 cgatttttgc ggtgtattaa tatttatgac ctggatcaca aaccgatttt ttagctggtg
     1741 cgaaatcggc atcaaaacca tgattctttt actgttgttg atggataccg gggccgtcag
     1801 ggctcaacga aatgaactgg tgatggccac cacattctca cccggagcta ccgcgtggat
     1861 aatacagcgc tggcaaacag agcctgagtc ggtaatgatc cgtacgctta accgcaccag
     1921 tgcctcactg gaacagttgc tttatacggc caacgtagaa aacgtcgatc tgatcctgac
     1981 ctcatcacca atgctgctcc agcaccttca ggagcaccag aaactggccc cgtttgatga
     2041 tgcacccgca gaaagccaaa acctggtgcc ggagtcgatc cgtgcaacct ccgttgcagt
     2101 agcaatatca ggttttggtc tgcttattaa tcgtcctgcg ctttctgtaa aacaccttcc
     2161 tgcccctgct gactgggacg atcttgcttt gccgatctat caggacgctt tattgatgag
     2221 tagtccgtcg cgttcagata ctaaccattt aatggttgag tcattactac agcaaaaagg
     2281 ctgggtgaaa ggatgggaaa cgttgctgac aagtgcagga aacctggtga caatctcctc
     2341 ccgcagtttt ggcgtagccg ataaaataaa aagcggactc ggcgttgccg gccccgttat
     2401 cgataactat gctaatttac tgttaaatga cccccatctc tcttttacct atttcccccg
     2461 gtctgcggta tctcccacct acgttgcaat tctcaagaaa agcccacacg ccgatgcagc
     2521 ccgtcgcttt attcactacc tgttaagtcc gaaaggacag cgtattctgg ccgatgccaa
     2581 tacaggaaaa tatccggtga caccacttgc cgcagacaat ccgcgggcaa cgcaacaaca
     2641 gctcctgatg aatcaacccc cgctgaatta tcaccttatc ctgaaacgtc agagtctggt
     2701 gcagcgcttg tttgatacgg caattagctt tcggttagca cagctaaaag atgcctggcg
     2761 agcgctgcac agtaccgagg cacgcctgaa gaaaccatta ccagaaatac gtgcactgtt
     2821 aacacaagtg cccgttgcag cggccagcag tgaagatccc gtctggttgg cacagttcga
     2881 taacaaaagt tttaccgagc agcaaatgat gaaatggcaa ctctggtttc tgaataacca
     2941 gcgcctggcg ataaaaaaac tggaagagct gaaatgaaaa acataacgct gtggcagcgt
     3001 ttaagacagg tcagtatcag taccagctta cgttgcgcat ttctgatggg ggcactactg
     3061 accctgattg tcagtagtgt cagtctgtat tcatggcacg aacaaagctc acaaattcgt
     3121 tactcgctgg atgagtattt tccccgtatt cactctgctt tccttattga agggaacctg
     3181 aatctggtgg tagaccagct aaatgaattt ttgcaggctc ccaacaccac ggtgcgattg
     3241 caacttcgta cccagattat tcagcatctc gacaccatag aacggcttag tcggggactg
     3301 tcatcccggg aacgccaaca actgacggtc attttgcggg acagtcgatc actgttatcc
     3361 gagttggatc gtgcgcttta caacatgttt ttactacggg aaaaggtgag tgagctatca
     3421 gcgcggattg actggttaca cgatgacttt actaccgagc ttaattcttt agtgcaggat
     3481 ttcacctggc agcagggaac gctgctggat caaatcgcct cccgacaggg cgatacggcg
     3541 caatatctga agcgatctcg tgaagtgcaa aatgaacagc agcaggttta taccctggca
     3601 cgcattgaaa atcagattgt tgacgatctg cgtgacagac tcaatgagct caaatcagga
     3661 cgtgatgacg acacacaggt ggaaactcat ctccgttatt ttgaaaatct gaaaaaaacg
     3721 gcagatgaaa atatacgtat gctggatgac tggcctggca ccattaccct gaggcagact
     3781 atcgatgaat tgctggatat gggaatcgta aaaaataaaa tgccggatac gatgcgtgaa
     3841 tatgtcgccg cccaaaaagc cttagaggat gccagtcgca ccagggaagc gacactgggt
     3901 cgcttcagaa cgttactgga agcgcagctt ggcagtactc atcaacaaat gcagatgttt
     3961 aatcaacgaa tggaacaaat tgttcgcgtt agcggtgggc tgatcctagt ggcgacagca
     4021 ctggcgttac tgctggcatg ggtattcaac cattatttta tccgctcacg gttggtgaaa
     4081 cgctttaccc tactgaatca ggccgttgtg caaattggtc tgggaggcac ggaaacgact
     4141 attcctgttt atgggaatga tgaactgggg agaattgcag gattattacg ccatactctc
     4201 gggcagctca atgtgcaaaa acagcaactt gaacaagaaa ttaccgatcg aaaggtgata
     4261 gaagccgatc tgcgtgccac ccaggatgaa ctgattcaga cagcaaagtt ggcggtagtc
     4321 gggcaaacga tgaccacgct ggcccacgag atcaatcagc cgctaaatgc actgtcaatg
     4381 tatctgttta cagcccgcag ggccattgaa cagacccaga aagaacaggc cagcatgatg
     4441 cttggtaaag ccgaaggggt gattagtcgt attgacgcca ttatccgttc actacggcag
     4501 tttacccggc gcgccgaact ggaaacatca ctccatgccg ttgatttagc acagatgttc
     4561 agtgcggcct gggaacttct agccatgcgt catcgctctc tgcaagctac gcttgttctg
     4621 ccgcaaggta cagccacagt ttcaggcgat gaggtcagaa cccagcaggt actggttaac
     4681 gtactggcga atgcgcttga tgtttgtggg caaggcgctg tcattaccgt taactggcaa
     4741 atgcagggta aaacgctgaa cgtattcatt ggcgataatg gcccgggctg gcctgaggca
     4801 ttgttgcctt cgttattgaa gccgtttacc accagtaaag aagtaggact gggtattggt
     4861 ctttcaattt gtgtgtcgtt gatggagcaa atgaaagggg aattgcggct ggcatcaacg
     4921 atgaccagga atgcctgtgt ggtactgcaa ttcagactaa cggatgtgga agatgctaag
     4981 tagtgaatat tcgattctgc tgattgatga tgatgctgac gtgctggacg cctacacaca
     5041 gttacttgaa cagtcgggct atcgtgtttt tgcctgtaac aatccttttg aggcacaggc
     5101 gtggatacaa ccagactggc caggcattgt gctaagtgat gtgtgtatgc ctggctgctc
     5161 tggtatcgat ttgatgatgt tattccacca ggatgatcaa cagctcccca ttctgcttat
     5221 caccgggcac ggcgatgtgc caatggcagt tgatgcggtg aaaaaaggcg cgtgggattt
     5281 tttgcaaaaa ccggtcgatc ccggcaaatt gctttctctg gttgaagagg cactacgcca
     5341 gcgtcaatca atcattgcgc gtcgtcagta ttgccaacag acattgcagg tggagttgat
     5401 agggagaagt gaatggataa atcagtatcg gcgacgttta cagcagttgt ctgagacaga
     5461 tattgccgtc tggctctacg gtgcccctgg cacaggacgg atgaccggtg cacgttatct
     5521 gcaccaattt ggacgtaatg cacaaggcga atttgtctac cgcgagctga caccagacaa
     5581 tgcaccacag cttaatgatt tcattgcgct ggctcagggg ggaacattag tgctcagcca
     5641 tcctgaacac ctgacgcgtg aacagcagta ccatctggtt cagttacaaa gccaggaaca
     5701 cagacctttt cgcctgattg gtattggaga cacctcgctg gtcgagctgg ccgccagcaa
     5761 ccatatcatc gcggagctct attactgctt tgcaatgaca caaatcgctt gcctgccatt
     5821 aactcaacgc cccgacgata ttgaaccact gtttcgccac tacctttgta aggcatgtca
     5881 acggcttaac catcctgttc cggaagtggg aaaagagatg ctcaaagaga tgatgcgccg
     5941 catgtggcca aataatgtgc gagagctggc caatgcagca gaactgttta ctgtaggaat
     6001 attaccgctg gcagaaacag caaacccgtt gatgcacgtc ggaaccccgg caccgcttga
     6061 caggcgagtg gaggacgctg agcgacagat aatcacagag gcgcttaata tccatcaggg
     6121 gcgtatcaat gaggttgctg aatatttaca aatcccgcgg aaaaaactct atctcagaat
     6181 gaaaaaatac ggtcttagca aggagcatta taaaaattaa atatcagtct atccagcaca
     6241 gaaattatgg agccccccag tcttggtacc agtcccgggt actgctgtaa gtttctgcac
     6301 cgattcagta aatttcagca gggataaaat agtgcagata atcgaagcat tacttccaga
     6361 gtggggaaca tccagagttt tgttcacaaa gtggtggagc actggctaca tctgttgata
     6421 tttcactcca ctctccactc tccactctcc actctccact ctccactctc cactctccac
     6481 tctccactct ccgccctcca ttcgactatg agactacaat aactcagttc gcctggcgct
     6541 gattattctg gccagttttg taaccggagt gtcatgtaca tgatccaaca gaaaatggcg
     6601 tgacgctcaa tacttctcag gtcagttact atattactca gcgtaagaaa ggtataaccc
     6661 agcatatctc ggccatgaaa gcgggtatct cagtccgttc tggtcgtcgg atcgaaaaag
     6721 gccagagggc aaaaaatagt gttcggcact ggagcacacg caaagatcct ctggaagcag
     6781 tgtgggacag catgcttgtt cctctgttga aagagaggcc tgttctgaca ccaacaactc
     6841 tgctggagat gctacaggat aaatatcccg gccagtatcc caacagcttt cgaagaacaa
     6901 tgcaacggcg gggcgcgaat ggaaattaca gtctggtgca gagcaggagg tcatgttccg
     6961 ccagtggcat cagcccggtc tgcgaggcct gttggacttt accaaactga aaggtgtggt
     7021 ggtcactatc gccggtaagt tgttggtgca tatgttgtat aacttccgtc tggaatggag
     7081 ccactggagc tgaatgcgag ttgtgctggc tggtgagagc ttctctgctc tggctgaagg
     7141 tctggcagga agccctcgga caactggggg gagtgccggc agaacataaa acggacagcc
     7201 tgagggcggc atggaaacaa caaggcaaaa gatggaggcc gcgagttgac tgagcgctat
     7261 gctgctctct gtcagcacta cggaatgcag ggcgtacacg ataatgccgg ttggggccac
     7321 gaaaatggct cggttaaaag tgcccacaga catctaaaaa ggcgtatctg tcaggcactg
     7381 atactgcggg gcagtaactc cttcagcacc atggaagaat atcaggcctt catcactcag
     7441 caggttatgc ggcacaaccg taaaag
//
LOCUS       DADSZD010000187         7397 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.189, whole genome shotgun sequence.
ACCESSION   DADSZD010000187 DADSZD010000000
VERSION     DADSZD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.189"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..585
                     /locus_tag="J1Z28_003864"
     CDS             <1..585
                     /locus_tag="J1Z28_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718647.1"
                     /translation="DLKDDSFEHFENGLQIIGFACGIWYADDMISDIKNGLAIDPDYE
                     FRLKPVKIRDNVLMATASYEGNKVIDIPFEDLLIMRRDLELIWKSVKTGIPMPSLLTK
                     RISSEPKIPKRLNNTAEHHAKNREGLLNAAIYILAKYPSECRGERKEISPEKWTNAII
                     KHLGELPPIFITNEQEILRKLRLAVNHAGLKQKG"
     gene            688..903
                     /locus_tag="J1Z28_003865"
     CDS             688..903
                     /locus_tag="J1Z28_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBA7718648.1"
                     /translation="MTLKTYPITGHARPRATADFLQISTVTLWRWEKNNPEFPKSIRI
                     SKRVSVYDAQEIRQWVKAQSAGLEEVV"
     gene            complement(953..1147)
                     /locus_tag="J1Z28_003866"
     CDS             complement(953..1147)
                     /locus_tag="J1Z28_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718649.1"
                     /translation="MGVIDVSTHKNERRGNPPFQFRLDPELRELMEQAQQQDGDESLA
                     AWIKRILRKELQSRGLEPKN"
     gene            1264..1440
                     /locus_tag="J1Z28_003867"
     CDS             1264..1440
                     /locus_tag="J1Z28_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBA7718650.1"
                     /translation="MAKQKCTWLFAAINRSQRNARPVMLRITADNERSARRRLAPDYV
                     LSFAGRIPCGGEHA"
     gene            1433..1792
                     /locus_tag="J1Z28_003868"
     CDS             1433..1792
                     /locus_tag="J1Z28_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718651.1"
                     /translation="MRNYPHPGERWQHERGWTVTIIRLIEASPSVALVNPEFSCEVLI
                     RHDSDNQLSSCPLAWFEQRYTRLFDVPLFKPAPAPAGGSGSGPLTLHPSVLFIRWRER
                     SIWRSAEPDDGHYSKFL"
     gene            1824..2108
                     /locus_tag="J1Z28_003869"
     CDS             1824..2108
                     /locus_tag="J1Z28_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718652.1"
                     /translation="MNISNGHNGAQGQTLPEKHNTGAFAYGLSEDGFGKLTRARNACD
                     MLQLLFSEYPSQAGALDAGCAPGVAALMEYLRADLTDIAHSCALIEGGAR"
     gene            2105..2488
                     /locus_tag="J1Z28_003870"
     CDS             2105..2488
                     /locus_tag="J1Z28_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HBA7718653.1"
                     /translation="MNQPQVSIFPAEMTTALYRRAIASAWRQKMLNETGNDQYGPHSL
                     TVERIEMAIALHIECALINEYGEAQGAAAALALLTDMLEPSLLTAPPVLTARGCEVMA
                     EMYRTLPAAFDVCCSTGVTLYQGEV"
     gene            2485..5157
                     /locus_tag="J1Z28_003871"
     CDS             2485..5157
                     /locus_tag="J1Z28_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HBA7718654.1"
                     /translation="MTIQTVTQISAAARGKWPVILQMLRIDVPENGRHGPCPKCGGKD
                     RFRLDDLDGRGTWICSQCGNGDGLDLVKLVTGYSVRKAAQEVAQVLNVPDVQELPVKP
                     ARQKAPKRDMGLTVAALMKESHTGESAYLTGKGFAGYPASLTGSVQHISGKDFPAGSL
                     LLPLTTNTGAVTGAQLIAPTGEKSILPGSTMKGAFVSLSPLPSEPPVQVVITEGYATA
                     LTVSQLTAGCVVAAISAGNLPNVAQSLRARWPEVKIIIAGDNDFQDGGENPGRAFAER
                     AAKVVGGWMTLPPGEIKADWNDFHREHGITRAREAFRNGLVLCGEGRTQLPHGFRLTQ
                     EYLWYEKQVQRNGETEIQNVKICSPLRVTAITCDADGGNFGRLLEWEDTWGERRRWAM
                     PMEMLSGSGEELRRVLLVNGLSYISTTGEARARLMEYISLCKPERRVTCVSRTGWHGQ
                     VYVLQDEVSGEGAEGVILQTTSVQGRDFRVSGTTEEWRENVSRYCTGNSRVAFAVSLA
                     FAAPLLRLVGMDGGGYHLKGESTDGKTTTMKAATSVCGGPDYWQTWRATGNALEGCAS
                     RRNDAAMMLDEIREVDGREAGNIAYMLANGQGKGRAGTDGELRTRKQWRLLFFSTGEL
                     SLTEHAAKAGERTFAGMEVRMIQIPSDSGKFGVFEELHGFDSGKALAEHLEWATSCYY
                     GSPFREWLKALTADLNGLTAQAKSLMKEYAAALTPKDAGNQVGRAVNRFALVAMAGEL
                     ATRLGITGWPEGEALRATRVCLNAWLKDRGHTANQEDIAALEQVRSFFTANQYSRFAD
                     WYDERNRPGNMVGWRRVEKGSTAQGTEAVTTFYVMPSGWKEICRGFDPRKVARLCADR
                     GYLLPSTDGKLQTTIRPPEMNPRRLYVFNSEVPG"
     gene            5558..6301
                     /locus_tag="J1Z28_003872"
     CDS             5558..6301
                     /locus_tag="J1Z28_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation initiation protein"
                     /protein_id="HBA7718655.1"
                     /translation="MNNTAVNNNLSTFPAVTQRALETMNTARNAWLEARRQQKAAADN
                     IATIRQRRAEMEATTNTLNEEWRTLFRESQGVVSKEMKKLRTEIALGRETLEDFDELL
                     AAQESENALLPQKAAELAGKYIHAHNILVDIRAKQIWEDFMQSHGKALIQTLSLLKST
                     MGREASAVVGVVNSVNDPDTVLKDFIHKHITRPALANDAMPEQDPVFKLAGIAPDYAA
                     RLDFSNQLSPAAMHKIKVRQELAEKEKAV"
     gene            6298..6849
                     /locus_tag="J1Z28_003873"
     CDS             6298..6849
                     /locus_tag="J1Z28_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HBA7718656.1"
                     /translation="MTARGTPDQALTSFRNARILWAGHSESRKAVEQQIASLLAATEK
                     PADYARQLELLRERLDVLKWQINCAARECIYSQHLLMEACTEAALSNFMQANGAALTS
                     ALAPFLKGRGGVDVASRILRSALVRQLTITPPEIAGDYREILDESGLMPDPGMIRDCQ
                     GSYTPAQHLRFQQRLNDINDIQE"
     gene            6855..7127
                     /locus_tag="J1Z28_003874"
     CDS             6855..7127
                     /locus_tag="J1Z28_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HBA7718657.1"
                     /translation="MALKCPECGTVAHARTAAYEAPSVKRSWYQCQNLECSCTFTALE
                     SVDKIIMKPRRNEQLSDKAQSPEKQQQTLNRYGSASKLSSRQQIPV"
BASE COUNT         1878 a         1824 c         2059 g         1636 t
ORIGIN      
        1 gaccttaaag atgactcctt tgaacatttt gaaaatgggc ttcaaattat aggatttgcc
       61 tgtggtattt ggtacgccga tgatatgata agtgacataa aaaatggatt agctatagat
      121 ccagattatg aattcaggct taaaccggta aaaatacgag ataatgtttt gatggcaacg
      181 gcctcatatg aaggaaataa ggtcattgat attccatttg aagatcttct tatcatgcga
      241 agagatttag agttaatatg gaaatcagta aaaactggaa ttcctatgcc aagtttactt
      301 acgaaaagaa tttcatctga acctaaaatc cccaagcgat taaataacac tgctgagcat
      361 catgcaaaaa atagggaggg gttattaaat gcagccatat atatattagc aaaataccca
      421 agcgaatgtc gtggtgaaag aaaagagata agtcctgaaa agtggactaa cgccataatt
      481 aaacatttag gagagctacc tccaatattt atcactaatg aacaagaaat cttaagaaaa
      541 ttacgattag cggttaatca tgcgggatta aaacaaaagg ggtagactga aacaatctac
      601 aggtagagtt gattacacta ccttcacagc attttgatat ccccatagta tctgccgtgt
      661 tcattacata acacggcagg taatttcatg acattaaaaa cttatcctat cactggacat
      721 gcccgcccac gagctactgc tgactttttg caaataagta cggttactct ctggcgctgg
      781 gaaaagaaca atcctgaatt tcccaaatct atacgcattt caaaacgggt atctgtttac
      841 gatgcacaag aaattaggca gtgggttaaa gctcaatcag caggattgga ggaagtagtg
      901 taatgacaat attaacaaag ctggggagaa aaaagcctcc ccaacgtatt aatcaattct
      961 ttggttctaa acctcggctt tgtaactctt tgcgtagaat gcgcttgatc caagccgcta
     1021 gcgattcatc gccatcctgc tgttgtgctt gttccatcag ttcacgcagt tcagggtcaa
     1081 gtcggaactg gaatggcgga ttcccacgtc tttcattttt gtgtgttgac acgtcaatta
     1141 cacccattgt aatgtattta tgtgtaatga cacattacac acagaaaaac aaaatagcaa
     1201 cgccccggag tgtttgcagc accaccgagg cgtctgacca gcacgttata tgaggtaacg
     1261 aagatggcta aacagaagtg tacctggtta tttgcggcga tcaaccgcag tcaacgtaat
     1321 gcccgtccgg tgatgttaag gatcaccgca gataacgagc gatcagcgcg gcgcagactt
     1381 gccccggact atgtactgag ctttgccggg cgcattccct gcggaggtga acatgcgtaa
     1441 ttatccgcat cccggcgaac gctggcagca tgaacgtggc tggacggtca cgattatccg
     1501 attaatagaa gcctcgccct ctgtggcgct ggttaatcct gaattcagct gtgaagtgct
     1561 gatacgtcat gacagcgaca accagctttc ttcctgccct ctggcatggt ttgaacagcg
     1621 atatacgcgc ctgtttgatg taccgctctt taagcctgca ccagctcctg caggcggtag
     1681 tggttccggc ccgctgacac tgcatccgtc tgtgctattt atccgctggc gggagcgtag
     1741 catatggcgt tctgctgagc ctgatgacgg tcactattca aagtttctct gacaaaacac
     1801 cgctgaaaac ggagtaatcc acaatgaata tttcaaacgg gcacaacggt gctcagggcc
     1861 agactttgcc tgaaaaacac aataccggtg cttttgccta tgggttgtca gaggacggtt
     1921 tcggaaagct gacccgcgca agaaatgcct gcgacatgct ccagcttctc ttttctgaat
     1981 acccgtctca ggccggagca ctggacgcag gctgtgcgcc tggtgtcgcc gcgctgatgg
     2041 agtacctgcg ggcagatttg acggatattg cacacagctg cgcactgatt gaaggaggtg
     2101 ccagatgaac cagccgcagg tttccatttt cccggcagaa atgaccaccg cgctttaccg
     2161 gcgggctatt gcttcagcat ggcggcagaa aatgctgaac gaaactggca atgatcagta
     2221 tggtccgcac agtctgacgg tggagcgtat tgaaatggcc attgcgctgc atatcgagtg
     2281 cgcactgatt aacgagtacg gcgaagcaca gggcgccgct gccgcgctgg cactgctgac
     2341 tgacatgctg gagccgtcac tgctgaccgc gccgccggta ctgaccgcgc gcggttgtga
     2401 agtaatggcg gagatgtacc gcacgcttcc ggcggccttt gatgtctgct gcagtaccgg
     2461 tgtgacgttg tatcaggggg aagtatgaca atacagacgg ttacgcagat ttcagccgcc
     2521 gcgcggggga aatggcccgt tattctgcag atgctgcgta ttgatgtacc tgaaaacggc
     2581 aggcacggtc cctgcccgaa atgtgggggc aaggatcgtt ttcgccttga tgaccttgac
     2641 gggcgcggga cgtggatctg cagccagtgt ggcaatggtg acggtctgga tctggttaag
     2701 ctcgtaaccg gttacagcgt cagaaaggct gcgcaggagg tggcgcaggt gcttaatgtg
     2761 ccggatgtgc aggaactgcc tgttaagcct gccagacaaa aagcccctaa acgcgacatg
     2821 ggtcttaccg tggcggcgct gatgaaagag agccacacgg gagaaagcgc ctatctgaca
     2881 ggaaaagggt tcgccggata cccggcctct ctgaccggaa gcgtgcagca tatcagcggt
     2941 aaggattttc ctgccggttc cctgctgtta ccactcacga ccaacaccgg agccgtgacc
     3001 ggtgcacagc ttatcgcccc gacgggtgaa aaaagcatac tgcccggcag cacgatgaaa
     3061 ggcgcgtttg tgtcgctcag cccgttaccg tctgaaccac cggtacaggt ggtgattacc
     3121 gaaggttacg ctacggcgct gacggtaagc cagctcactg ccggatgcgt agtggctgcc
     3181 atatctgcgg gcaacctgcc caatgtggcg cagtcgctgc gggcacgctg gcctgaggta
     3241 aaaattatca tcgccggtga taacgatttt caggatgggg gggagaatcc cggcagagcc
     3301 tttgctgaac gggcggcaaa agttgttggc ggctggatga cgctgccacc aggagagatt
     3361 aaggctgact ggaatgactt tcatcgggag cacggtatta cccgtgcccg tgaagccttt
     3421 cgcaacggtc tggtactgtg cggggaaggc cgcacgcaac tgccgcacgg gttccgtctt
     3481 acccaggaat atctctggta tgaaaagcag gtacagcgca acggtgagac ggagatccag
     3541 aacgtcaaaa tatgcagccc gctgcgcgtg acggcaatca cctgcgatgc cgatggcggt
     3601 aacttcggac gactgctgga atgggaagat acgtggggtg agcgtcgccg ctgggcgatg
     3661 ccgatggaaa tgctgagcgg cagtggtgag gaactgcgcc gggtactgct ggttaacggg
     3721 ctgtcttata tcagcactac cggtgaggca cgcgcacgcc tgatggaata tatctcactg
     3781 tgtaaaccgg aacgccgcgt gacctgcgtc agccgtactg gctggcatgg tcaggtttac
     3841 gtcctgcagg atgaggtcag cggtgaaggt gcagagggtg tcattctcca gaccacttcc
     3901 gtgcaggggc gtgatttccg cgtgtcgggc acaacagagg aatggcggga gaacgtatcc
     3961 cgctactgta ccggcaactc tcgcgtggca tttgctgtca gcctggcctt tgctgcacca
     4021 ctgttacggc tggttggtat ggacggcggc ggctaccacc tcaaagggga atcgacagac
     4081 ggtaagacca ccaccatgaa agcggcaacc tccgtctgcg gcgggcctga ctactggcag
     4141 acatggcggg ccaccggcaa cgcgctggag ggatgcgcca gccgccgcaa cgatgccgcc
     4201 atgatgcttg atgagatccg ggaagttgac ggacgcgagg caggcaatat cgcctacatg
     4261 ctggcaaacg gtcagggcaa gggccgtgcc ggtacggacg gtgagctgcg tacccgtaag
     4321 cagtggcgcc tgttgttctt ttcaactggc gagttgtctc tgaccgaaca tgcggcaaag
     4381 gccggtgagc gtacttttgc cgggatggaa gtcaggatga tccagatccc cagcgattcc
     4441 gggaaatttg gcgtttttga ggagctgcac ggcttcgaca gcggcaaggc tctggcagag
     4501 catcttgaat gggccacgtc ctgctactac ggttcgccgt tccgggagtg gctgaaagca
     4561 ctgaccgctg atcttaacgg actaacggca caggcaaaat cgctgatgaa ggaatatgct
     4621 gccgccctga ctccgaaaga tgcaggcaac caggtgggcc gggctgtgaa tcgctttgca
     4681 ctggtggcga tggcgggtga actggcaacc cgtctgggta tcaccggctg gcctgagggt
     4741 gaagcactgc gggcaacccg cgtctgcctg aacgcatggc tgaaagatcg cgggcacacc
     4801 gccaatcagg aagatattgc cgcactggag caggttcgca gtttctttac cgcgaatcag
     4861 tacagccgct ttgcagactg gtatgacgag cgcaaccgcc ccggcaatat ggtgggctgg
     4921 cgcagggtgg agaaaggcag taccgcgcag ggcacggaag ctgtcactac gttctatgtc
     4981 atgccgtccg gctggaaaga aatctgcagg ggatttgacc cgcgcaaggt ggcgcgtctg
     5041 tgcgcagatc gtggatacct gctgccctcc actgatggca aactccagac aaccattcgc
     5101 ccgccagaga tgaatccccg caggctctat gtcttcaaca gcgaggtgcc gggttaaggc
     5161 tttgcgtgag tcttatttat taagggtaac aggtgaaaca ggtgaaacaa ccgtatttta
     5221 cagggctgaa cctgttccac cttctgaaat agtgaggtgg aacagatgta acgccagcca
     5281 atccgctgtt ccaccttgtt acccgcgatg ttccaccggt gcagtgtata taagtgcttt
     5341 ataaaacaaa gctgtaacac atgtttcacc tgttccaccg catcagggac ataaagcaga
     5401 ctgaaactga gggcattttt atctggctgg cttttatagc cacgtctgaa taaccgcaag
     5461 cgcccgttgt gccagccacc acacactgag taccgatagt gtgcttgtgt cagccacgac
     5521 acaattgacg taacgaacca acccgacagg agaagtcatg aacaacaccg cagtgaataa
     5581 caacctttcc acttttccgg ctgtaacgca gcgggcactg gaaaccatga acacagccag
     5641 aaacgcatgg cttgaagcac gtcgtcagca gaaagcggca gcggataata ttgcgacaat
     5701 ccgccagcgt cgcgctgaga tggaagccac gacgaacaca ctgaatgagg agtggcgcac
     5761 gctgtttcgt gaaagtcagg gcgtggtttc aaaggaaatg aaaaaactgc gcacggaaat
     5821 tgcgctggga cgagaaacgc ttgaggattt tgatgagctg ctggcggctc aggaaagcga
     5881 aaatgcactt ttgccgcaga aagctgcgga attagccgga aagtatatcc acgcgcataa
     5941 cattcttgtg gatattcgcg caaaacaaat ctgggaagat tttatgcaat cgcatggaaa
     6001 agcgcttatt cagacgctga gcctgcttaa atccacaatg ggccgggaag ccagtgccgt
     6061 tgtgggtgtg gtgaattcag ttaatgaccc ggacacagtg ctgaaagact ttattcataa
     6121 acatatcacc agaccggctc tggctaacga tgcgatgcct gaacaggacc cggtgtttaa
     6181 actggcggga attgccccgg attatgcggc gcgtctggat ttcagtaatc aactctctcc
     6241 ggcagctatg cataaaatta aggttcgtca ggaacttgct gagaaggaga aagcagtatg
     6301 acggcccgag gcacgcctga ccaggcactt accagttttc ggaatgcccg tattctgtgg
     6361 gccggacata gtgagagccg aaaagccgtt gaacaacaga tagcatctct cctcgctgcc
     6421 acagaaaaac cagcagatta tgcccgacag ctcgaattac tgcgggaacg tctggatgtg
     6481 ctgaaatggc aaattaactg tgctgcgcgc gaatgcattt actcgcagca tttactgatg
     6541 gaagcctgca cagaagccgc tctcagcaat tttatgcagg cgaatggggc agccctgaca
     6601 tctgcactgg ctccgttcct gaaggggcgc gggggggttg acgttgcgtc ccgtatatta
     6661 cgcagtgctc tggttcgcca gctgacaata acaccgcctg aaattgccgg ggattatcgt
     6721 gagatcctgg atgaatccgg tttgatgcct gaccccggga tgattcgtga ttgccagggc
     6781 tcttacaccc cggcacaaca tctgcgtttt cagcaacgcc tgaatgacat taacgatata
     6841 caggagtgat attaatggca cttaaatgtc cggaatgcgg tacagttgca cacgccagaa
     6901 cagcagccta tgaagctccg tcagttaaac gctcgtggta tcagtgccag aatctggaat
     6961 gttcctgcac atttacggca ctggagagtg tggataagat aatcatgaaa ccccgacgca
     7021 atgaacagct atcagacaaa gcacaatccc cggaaaaaca acagcaaaca cttaatcgct
     7081 atggctccgc gtcaaaactt tcaagccgtc agcagattcc tgtctgatta gaaagaacgc
     7141 ccacgtaatc ccggtccagt accgggattt tttatcccct tttcctggct ggcatgagag
     7201 cgaatgagtg catgtctatg gcgcatgaaa acgcatgagt cttatgcacc atttttgacg
     7261 agaaaaccct tgtttggtgg cttctgagac gatttatgag gtgcatgaaa accagtctgt
     7321 taagcgaagc gggcaggcgt gcggggctgc gcacggaggc ggaataaata gtaatgctat
     7381 ggatgctggg cacacat
//
LOCUS       DADSZD010000188         7359 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.190, whole genome shotgun sequence.
ACCESSION   DADSZD010000188 DADSZD010000000
VERSION     DADSZD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.190"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            9..863
                     /gene="gatY"
                     /locus_tag="J1Z28_003875"
     CDS             9..863
                     /gene="gatY"
                     /locus_tag="J1Z28_003875"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBA7718658.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAASMHAP
                     VIIAGTPGTFTHAGTENLMALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            892..2154
                     /gene="gatZ"
                     /locus_tag="J1Z28_003876"
     CDS             892..2154
                     /gene="gatZ"
                     /locus_tag="J1Z28_003876"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBA7718659.1"
                     /translation="MKTLITRHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCADDSIPLAPETVAERAAVLCLAAESVATDCQ
                     REQLNYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTRTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2164..2616
                     /gene="gatA"
                     /locus_tag="J1Z28_003877"
     CDS             2164..2616
                     /gene="gatA"
                     /locus_tag="J1Z28_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBA7718660.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKIHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2647..2931
                     /gene="gatB"
                     /locus_tag="J1Z28_003878"
     CDS             2647..2931
                     /gene="gatB"
                     /locus_tag="J1Z28_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBA7718661.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGNIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            2935..4290
                     /locus_tag="J1Z28_003879"
     CDS             2935..4290
                     /locus_tag="J1Z28_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA7718662.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKAAIA
                     E"
     gene            4338..5378
                     /gene="gatD"
                     /locus_tag="J1Z28_003880"
     CDS             4338..5378
                     /gene="gatD"
                     /locus_tag="J1Z28_003880"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBA7718663.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRDLRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            5478..6257
                     /locus_tag="J1Z28_003881"
     CDS             5478..6257
                     /locus_tag="J1Z28_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7718664.1"
                     /translation="MTMNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRF
                     LEQKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMT
                     VILDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMH
                     GSIAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKF
                     NRRGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6339..7238)
                     /gene="yegS"
                     /locus_tag="J1Z28_003882"
     CDS             complement(6339..7238)
                     /gene="yegS"
                     /locus_tag="J1Z28_003882"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBA7718665.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRITWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1834 a         1649 c         1932 g         1944 t
ORIGIN      
        1 atgtgaaaat gtacgtggta tcgacaaagc agatgctgaa caacgcgcag cgcggcggtt
       61 atgcggttcc ggcattcaat attcacaatc tcgaaacgat gcaagtggtg gtggaaaccg
      121 ctgccagcat gcatgcgccg gtcatcatcg ccggaacgcc cggcacattt actcatgccg
      181 gtacagaaaa tctgatggcg ctggtcagcg cgatggcgaa gcaatatcac catccactgg
      241 cgattcatct cgaccatcac acgaaatttg acgatatcgc tcagaaggtt cgttctggcg
      301 tgcgctcagt catgattgac gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca
      361 aagaggtggt ggatttttgc catcgctttg atgtcagcgt cgaagcggag ctggggcaac
      421 ttggcggcca ggaagatgat gtgcaagtca atgaagccga tgctttttat accaaccccg
      481 ttcaggcgcg tgaatttgcc gaggcaaccg gaattgattc cctggcggtc gccatcggca
      541 cggctcatgg gatgtatgcc agcgcaccgg cgcttgattt ttctcgactg gagaacattc
      601 gccagtgggt gaacttaccg ctggtgctgc atggcgcgtc aggattatcg acaaaggata
      661 ttcagcaaac catcaaactg gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg
      721 ccttctcgca ggcgttaaaa aattacctga ccgagcaccc cgaagcgacc gatccccggg
      781 attatttgca gtcggctaaa tcagcaatgc gcgatgtggt gagcaaagtg attgccgatt
      841 gtggttgcga aggcagggca taacgcacct gccatttaac aaggaaaaaa catgaaaacg
      901 ttaattaccc ggcataaagc tggtgaacat atcggcatat gttcagtctg ttctgcccat
      961 ccgttggtta tcgaagcggc gctggcattt gatcgcaaca gcacgcgtaa agtgctgatt
     1021 gaagcaacgt caaaccaggt caatcaattt ggcggttata ccggaatgac accggcagac
     1081 tttcgcgaat ttgtttttgc gattgccgat aaagtcggat ttgctcgtga gcgtattatt
     1141 ctcggcggcg accatctggg accaaactgc tggcagcaag aaaatgcgga tgcggcgatg
     1201 gaaaaatccg tcgagctggt aaaggcatat gttcgtgccg gcttcagtaa aattcatctt
     1261 gatgcgtcaa tgtcctgcgc tgacgattcc ataccgttag cgccagaaac ggttgctgaa
     1321 cgtgccgctg tgctttgtct ggcggcggaa agtgtggcga ctgattgcca gcgtgagcaa
     1381 ctgaactatg tcattggcac tgaagttccg gttccgggcg gtgaggccag cgccattcag
     1441 tcagtacaca tcacccaggt tgaagatgcc gccaatactt tacgcacgca tcaaaaggcc
     1501 tttattgccc gtggtctggc agaggcgtta acacgcgtga ttgccatcgt ggtgcagccg
     1561 ggtgtggaat ttgaccatag caatattatt cattatcagg cgcaagaagc tcaggcgttg
     1621 gcgcaatgga tagaaaaaac caaaatggtt tatgaagcgc attccaccga ttaccagacc
     1681 cggacggcct atcgggaatt agtccgcgat cactttgcaa tattgaaagt cggtcccgca
     1741 ttaacctttg ctttacgtga ggcgatattt gcgctggcgc aaattgagca ggaacttatc
     1801 gccccagaaa atcgcagcgg ttgcctggcg gtaattgaag aagtgatgtt tgacgaaccg
     1861 caatactgga aaaaatatta tcgtacgggt tttaacgatt cattactgga tattcgttac
     1921 agcctgtcgg atcgtattcg ttattactgg ccgcatagcc ggattaaaaa tagcgtcgaa
     1981 acgatgatgg tgaatctgca aggcatggaa atccctctgg gcatgattag tcagtatctt
     2041 cctaaacaat ttgaacgcat tcagtccggg gaattatcag caataccgca tcagttgatt
     2101 atggataaaa tttatgatgt tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg
     2161 tatatgacta acctgtttgt tcgtagcgga atttcttttg tcgatcgtag cgaagtttta
     2221 acccatatcg gtaatgagat gctcgcgaaa ggtgtggttc atgatacctg gccacaggca
     2281 ttaattgcca gagaagcaga attccctacc gggataatgc ttgagcagca cgccattgca
     2341 ataccgcatt gtgaggcgat tcatgctaag tcgtcagcca tttatctgtt aaggccaaca
     2401 aataaaattc attttcagca agcggatgat gataacgacg tggcggtatc gttggttatt
     2461 gcgttgattg tggaaaatcc gcagcagcaa ttgaaacttt tacgctgttt atttggcaag
     2521 ttacaacagc ccgagatcgt cgagacacta atcactcttc ctgaaaccca gttaaaggaa
     2581 tacttcacaa agtatgtttt agattcagac gaataaatcc ctctgtaaca ataaaaagga
     2641 ctatttatga aacgcaagat tattgtcgct tgcggaggcg cggttgcgac ctctacgatg
     2701 gcggcggaag aaattaaaga gttgtgtcag agtcataata ttcctgttga attaatccag
     2761 tgtcgggtta atgaaataga aacctatatg gatggcgtgc atttgatatg caccactgcc
     2821 agggtggatc gtagttttgg caatattccg ttagttcacg gcatgccttt tgtttctggt
     2881 gtcggtatcg aagcattaca aaataaaatt ctgactatct tacaggggtg acctatgttt
     2941 tcagaagtca tgcgttatat tctcgacctc ggccctacgg tgatgctgcc gattgtcatc
     3001 attatttttt ctaaaatatt aggcatgaag gcaggcgatt gctttaaagc gggtctgcat
     3061 atcgggattg gctttgttgg cattggcctt gtgattggct taatgctgga ttccattggc
     3121 ccggcggcga aagcgatggc ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc
     3181 tggccgggct cttcacccat gacctgggcg tcgcaaattg cgctggtggc gattccgatt
     3241 gcgattctgg ttaacgtggc gatgttactg acccgtatga cgcgggtggt aaatgttgat
     3301 atctggaata tctggcatat gaccttcacc ggcgcgttgc tgcatctggc aaccggttca
     3361 tggatgatag ggatggcggg tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac
     3421 tggtttgccc gcgatacccg aaatttcttt gagctggaag gcattgctat tccgcacggt
     3481 acgtcggcgt atatggggcc aattgctgtg ctggtcgatg ctatcatcga gaaaatccca
     3541 ggcgttaacc gaattaaatt tagcgccgat gatattcagc gcaaatttgg cccttttggc
     3601 gaaccggtca ccgtgggttt tgtgatgggg ctgattatcg gcatcctcgc gggttacgat
     3661 gtcaaaggtg tattgcagct ggcggtaaaa acggcggcag ttatgctgct gatgccacgg
     3721 gtgattaaac ccatcatgga tggtttaacg cccatcgcta agcaggcccg tagtcgttta
     3781 caggcgaagt tcggcggtca ggagttcctg attggccttg atccggcgtt gctgctggga
     3841 catacggcgg tggtatcggc aagcctgatt tttattccac tcaccatttt aattgctgtt
     3901 tgtgtgccgg gtaatcaggt gctgccgttt ggcgaccttg ccaccatcgg cttctttgtg
     3961 gcgatggcgg tcgcggtgca tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc
     4021 attatgagca tcaccctgtg gatcgcgacg caaactattg gtttgcacac ccaactggcg
     4081 gctaatgcgg gcgcgttaaa agccgggggt atggtggctt caatggatca gggcggttct
     4141 cccattacct ggttactgat tcaggttttc tccccgcaaa atattcccgg tttcattatt
     4201 atcggcgcca tttatctcac cggtattttc atgacctggc gtagggcgcg cggctttatc
     4261 aaacaagaga aagctgctat cgcagaataa tttttacctg agggggaggt tatccccctt
     4321 aacaatcagg agtttttatg aaatcagtgg tgaatgatac tgatggtatc gtgcgcgtag
     4381 cagaaagcgt cattcctgaa attaaacatc aggatgaggt gcgggtaaaa attgccagct
     4441 cgggcttatg tggttccgat ttacccagga tatttaaaaa tggtgcacat tattatccaa
     4501 taacgttagg ccatgaattt agtggctata ttgatgcggt gggatccggt gttgatgatt
     4561 tacaccccgg cgatgcggtt gcctgtgtgc cgttattacc ctgttttact tgtccagagt
     4621 gtctgaaagg gttttattcc cagtgcgcaa aatatgattt tattggctca cggcgtgatg
     4681 gtggatttgc cgaatatatt gtcgttaagc gaaacaatgt ctttgcacta cctgcggata
     4741 tgcctattga ggatggcgct tttattgaac cgattaccgt tggcctgcat gcgttccatt
     4801 tagcgcaagg atgtgagaat aaaaacgtta ttattattgg tgccggaacc attggcctgc
     4861 tggcaattca gtgcgctgtc gcgctgggag caaagagtgt gacggctatc gacattagct
     4921 cagaaaaact ggcactggca aaatctttcg gtgcgatgca aacatttaac agccgtgaaa
     4981 tgagcgcgcc gcaaatacag ggcgttttac gcgatctgcg ctttaatcag cttatcctcg
     5041 agacggctgg cgtcccgcaa accgtcgaac tggcggtaga gattgccggt cctcatgccc
     5101 aactggcgct ggtaggcacg ttgcatcagg atctgcattt aacatctgca acgtttggca
     5161 aaatattgcg taaagagctg acggttatcg gcagttggat gaactactcc agcccttggc
     5221 cggggcagga gtgggaaacg gcgagccggt tgctgacaga acgtaagtta agcctggagc
     5281 cattaatcgc tcaccgtgga agctttgaaa gcttcgccca ggcggtgcgt gacatcgctc
     5341 gtaatgccat gccgggcaaa gtgttgctca ttccctgaaa ccgcgggcca gcgtgatgca
     5401 ggcccggtat tgtgcaaaac agatcattca ccaatggtcc cccttcgttt acactagcca
     5461 caattgaata tgggtaaatg acgatgaatt cattcgagcg aaggaataag atcatccaat
     5521 tagtgaatga acagggaacc gtgcttgttc aggatctggc gggagtattt gctgcctcgg
     5581 aagcgacaat ccgtgccgat ttgcgctttc tcgaacaaaa aggcgtggtt acgcgctttc
     5641 atggcggtgc ggcgaaaata atgtccggta atagcgaaac cgagacccag gaagtcgggt
     5701 ttaaagagcg atttcagctc gccagcgcgc cgaaaaacag aatagcgcag gcggcagtca
     5761 aaatgatcca cgaagggatg accgttattc tcgacagcgg aagtaccaca atgcttatcg
     5821 ccgaaggatt aatgaccgcc aaaaatatca cggtgattac caacagtctc ccggctgcgt
     5881 ttgccctttc cgaaaataaa gacattactc tggtcgtctg cggtggcacg gtccgccata
     5941 aaacgcgatc tatgcatgga tctattgctg aacgttctct gcaggatatt aatgccgatt
     6001 taatgtttgt gggtgctgat ggtattgatg cggttaatgg tattacgacc tttaatgaag
     6061 gttattctgt tagcggtgcg atggtgacag cggcgaataa agtgattgcc gttcttgatt
     6121 catcgaaatt taatcgtcgc ggttttaatc aggtactgcc aatcgaaaaa attgacatta
     6181 ttattaccga tgatgctgtg tctgaggtgg ataaactggc attgcagaag acacgggtaa
     6241 aattaattac ggtgtagtat tgttctgtat gccggataag gtgtttacac cgtatccggc
     6301 attacggatt ctctctggtt caacagagaa tagtttgatt aacgcaacaa tggacaatct
     6361 ggtggtaatc gacaacgcaa cgccgccgga agtatttcaa tatggaagtt ttgcccactc
     6421 aacggttcgc catcaagatt aaaagtgatt tcgtgtgggg cttgtatatc aaaccacgac
     6481 gaagctcctt cgataatatt cgggttatct tcgtcagatt ttaaggttga tacgagagtc
     6541 ggaataattt catcgccggt aaaaatgcgc agttgcagca agccatcgtt aattaatgcg
     6601 ttcgggcaca attgttgacc gccaccggcc tgacgaccgt taccaatacc aatgaccagg
     6661 gcgtcacctt gccagtgaaa gttttcaccg cggatttcac aacggtccgg ttgcagagtg
     6721 tccatgcgca ttaagccatg aatgatgtaa gagacgccac ccagcgcggc ttttaatttt
     6781 tccggcgttt ctgtggtaat acgcgtccca aacccgcctg tcgccatatt aataaaacag
     6841 gtttgtttgt tgacctgcgc catatctatc gcaatggcgt taccggcaat tgccagtttc
     6901 agcgccttat ccagtgcctc aggaatccct acactggtgg caaaatcatt ggcagttcct
     6961 aatggtaaaa ttcccagcgc gggtatgtca tccccctcac attgaatcaa tgccgtagaa
     7021 acttcattaa tggtgccatc gccaccaccg gcaatcactg ttgcgacacc taacttccgg
     7081 gcctcctcta caaatcgtgc agcatcgcct ttctcccagg tgatccgcac atggatcgtc
     7141 attccttcct cacgcaacag cataattgct tcgcgcaagg gtagattgtc agtacttttg
     7201 ccattaagaa tcagtaagct ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt
     7261 ggtctgtaga tagtgtagag cagaaaagaa aaggtggagt cagaacagga tgaaagtcgg
     7321 aggataaaaa gtcagttcag gtatggggat tcagtgctt
//
LOCUS       DADSZD010000189         7349 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.191, whole genome shotgun sequence.
ACCESSION   DADSZD010000189 DADSZD010000000
VERSION     DADSZD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.191"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            188..2338
                     /gene="btsT"
                     /locus_tag="J1Z28_003883"
     CDS             188..2338
                     /gene="btsT"
                     /locus_tag="J1Z28_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBA7718666.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPI
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            2456..2659
                     /locus_tag="J1Z28_003884"
     CDS             2456..2659
                     /locus_tag="J1Z28_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBA7718667.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            2670..3626
                     /gene="yjiA"
                     /locus_tag="J1Z28_003885"
     CDS             2670..3626
                     /gene="yjiA"
                     /locus_tag="J1Z28_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBA7718668.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGYIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(3660..3962)
                     /locus_tag="J1Z28_003886"
     CDS             complement(3660..3962)
                     /locus_tag="J1Z28_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HBA7718669.1"
                     /translation="MSMVKHKRGTPSTLDVQHEAELKALANKSDEDIDYSDIPPSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(3959..4168)
                     /locus_tag="J1Z28_003887"
     CDS             complement(3959..4168)
                     /locus_tag="J1Z28_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718670.1"
                     /translation="MVDYPKRDAISNINAIIVDTFLYIQLGMATMPMEFEWNANKAKS
                     NRVKHGIRFEDAVLVLDDPQRTEPL"
     gene            4264..5430
                     /locus_tag="J1Z28_003888"
     CDS             4264..5430
                     /locus_tag="J1Z28_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBA7718671.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDTWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVNREGMSYRITDTGRAWLGKALPTRKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            5494..7113
                     /locus_tag="J1Z28_003889"
     CDS             5494..7113
                     /locus_tag="J1Z28_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HBA7718672.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTCGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRKNLEALGYGL"
     gene            7103..>7349
                     /locus_tag="J1Z28_003890"
     CDS             7103..>7349
                     /locus_tag="J1Z28_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBA7718673.1"
                     /translation="MGFDFQLLENAGIQLIDGDRGKNYPKHNDFMENGYCLFLSAKNV
                     TKSGFQFQETLFINETKDRELRAGKLKYGDIVLTTRGT"
BASE COUNT         1798 a         1912 c         1905 g         1734 t
ORIGIN      
        1 tttgccgctc accgccattc ctgaccgctt acgtccctaa aataaccact cagttattta
       61 ccttacttta cgcgcgcgta actctggcaa catcactgca ggatagcggt caatttacct
      121 cctcaaacgc aacgcaaacc tagaacggct tcggccaact attaatcaat acatgccagg
      181 ttttactatg gatactaaaa agatattcaa gcacataccc tgggtgattc tcggaatcat
      241 cggtgcattc tgcctcgcgg tagttgcatt acgtcggggg gagcacgtca gcgccctgtg
      301 gatcgtggtc gcctctgtgt cggtgtatct ggtggcgtat cgctactaca gtctgtacat
      361 cgcccagaag gtgatgaaac tcgaccccac gcgcgcgacg cctgcggtta ttaacaacga
      421 cggtctgaac tacgttccga ccaaccgtta cgtgttgttt ggtcaccact tcgccgctat
      481 cgccggtgct ggtccgctgg tgggtccggt tctcgccgcg cagatgggct acctgcctgg
      541 cacgctgtgg ctgctggcgg gggtcgtgct ggccggtgcg gtacaggact ttatggtgct
      601 gtttatctcc tctcgccgta acggtgcatc tctcggtgag atgatcaaag aagagatggg
      661 gccaataccg gggactatcg cgctgtttgg ctgtttctta atcatgatca tcatcctcgc
      721 cgttctggcg ctgattgtgg ttaaagccct ggccgaaagt ccgtgggggg tcttcaccgt
      781 ttgctcaacc gtaccgattg cgctgtttat gggtatctat atgcgcttta ttcgtccggg
      841 gcgtgtgggt gaagtctctg tcattggtat cgtgctgctg gttgcctcta tctacttcgg
      901 tggcgtgatt gctcacgatc cgtactgggg tccggcactg acctttaaag acaccaccat
      961 taccttcgcg ctgattggct atgcgtttgt ttccgcactg ctgccagtgt ggctgatcct
     1021 cgcaccgcgc gactatctgg caaccttcct gaaaatcggc gttatcgtcg gcctggcgct
     1081 gggtatcgtg gtgctgaacc cggaactgaa aatgcctgcc atgacccagt acattgacgg
     1141 tactggcccg ctgtggaaag gcgctctgtt cccgttcctg ttcatcacca tcgcctgtgg
     1201 tgcggtatct ggcttccacg cgctgatctc ttccggtacg acgccgaaac tgctggctaa
     1261 tgaaaccgac gcgcgtttca tcggctacgg cgcaatgctg atggagtcct tcgtggcgat
     1321 tatggcgctg gttgctgcgt ccatcatcga accgggtctt tacttcgcga tgaacacccc
     1381 gcctgctggc cttggcatca ccatgcctaa cctgcatgaa atgggtggcg agaacgcgcc
     1441 gatcatcatg gcacagctga aagacgttac cgcacacgcg gcagcgaccg tcagctcctg
     1501 gggcttcgtg atttcgccag agcagatcct gcaaaccgcg aaagacattg gtgagccttc
     1561 tgtcctgaac cgtgcaggtg gtgcaccaac gctggcggta ggtatcgctc acgtgttcca
     1621 caaagtgctg ccgatggctg acatgggctt ctggtatcac ttcggtattc tgttcgaagc
     1681 cctgttcatc ctgaccgcgc tggatgcggg tacccgttct ggccgcttta tgctgcaaga
     1741 cctgctgggt aacttcatcc cgttcctgaa aaaaaccgac tccctggttg ccggtatcat
     1801 cggtactgcg ggctgtgtgg gtctgtgggg ctacctgctg tatcagggcg tggtcgatcc
     1861 gctgggcggc gttaagagcc tgtggccgct gttcggtatc tccaaccaga tgctggcagc
     1921 cgtggcgctg gtactgggca ccgttgtgct gattaagatg aagcgcaccc aatacatctg
     1981 ggtaactgtt gttccggctg tatggctgct tatctgcacc acctgggcgc tgggcctgaa
     2041 actgttcagt accaacccgc agatggaagg cttcttctac atggcaagcc agtacaaaga
     2101 gaagattgct aacggtactg acctgacggc gcagcagatt gccaacatga accacatcgt
     2161 tgtgaacaac tacaccaacg caggcctgag tattctgttc ctgattgtgg tgtacagcat
     2221 catcttctac ggtttcaaaa cctggctgaa ggtgcgtaac agcgacaaac gtactgacaa
     2281 agaaacaccg tacgttccaa tcccggaagg cggcgtgaag atctcttcgc accactaacc
     2341 ggttgcctta tccggcttac aaaatgtcta tcgtaggccg gataaggcgt ttacgccgca
     2401 tccggcaaat ccccagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt
     2461 tggtaactta ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat
     2521 tccagactac gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat
     2581 gagctatgaa gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg
     2641 cggtatgcgc tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg
     2701 gttttttagg cgcaggaaaa accaccctgc tgcgccatat tcttaacgaa caacacggct
     2761 acaagattgc cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg
     2821 gcgatcgcgc cacgcaaata aaaacgctga ccaacggctg catctgctgt tcgcgctcca
     2881 acgagctgga ggacgcgcta ctcgacctgc tggacaatct cgacaagggc tatattcagt
     2941 tcgaccgtct ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct
     3001 ttttctccca tgaagtttta tgccagcgtt acctgctgga cggagtgatt gcgctggtgg
     3061 atgcggtaca tgccgatgag cagatgaacc agttcaccat cgcccagtca caggttggct
     3121 acgccgaccg tattctgctg actaaaaccg acgtcgcagg cgaagcagaa aaactgcgtg
     3181 aacgcctggc gcgcatcaac gcccgtgcac cggtctacac cgtcacccac ggcgacatcg
     3241 acctgggcct gctgttcaac accaacggtt ttatgctgga agaaaacgtc gtcagcacca
     3301 agccacgttt tcactttatc gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac
     3361 tggattaccc ggtagatatc agcgaagttt ctcgggtgat ggaaaacctg ctgctggaat
     3421 cggcagataa actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc
     3481 tgctgttcca gggcgttcag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg
     3541 aaaaaccgca tagcacgatg gtgtttatcg ggattcagtt gccagaggaa gagattcggg
     3601 cagcgtttgc ggggttgagg aaataatttt ggatgatggc cgccatgtag cggcctgaat
     3661 tattttttgt tctgatcgcg cagcatcgct tcacgtaaaa ttgcgttcag gcgagtttga
     3721 tagcctttac ctggtcgctt caaccattcc atcacatcag catcaatacg caccgaagcc
     3781 tgagttttca atggccgata aaacttaccg cgttcggcat ttgaccattg ctcatcgctg
     3841 gagggtggga tatcactgta atcaatatct tcgtcagatt tattcgccag cgccttcagt
     3901 tcggcctcat gctgcacatc gagtgtagag ggtgtccctc gtttatgttt aaccatgctc
     3961 atagcggctc cgttcgttgt ggatcatcaa gcactaacac cgcatcttca aagcggatgc
     4021 catgtttcac ccgattactt ttggccttgt ttgcattcca ttcaaactcc attggcatag
     4081 ttgccatccc taattgtata tacaaaaatg tatctacaat tatagcgttg atgttggaaa
     4141 tagcatcacg ttttggataa tcaaccatga aattgacaat gagtgagttg gacttgattc
     4201 atccaataga atttctcgcc tgacatggca cactaaacac ctcgctctcg ttaagggatt
     4261 accgtgaaaa actacaaagt cattaccccg ctttttccta cctatgcgca agttaaggct
     4321 atgatgaaag ccgtctcagg ctattccctg aaagcggtac gcaatatgat taccgccatt
     4381 cacgagcaaa ccggtacgcc gcaaaaaccc gttgactggt cggaacctga cctgtggatc
     4441 agcgaacgct taacgggtga agatgccgac atcgcccgtc gtatttggga taccgataat
     4501 cacatattga acccgcgcca cagctacggc tgctacttat ttctcaacta tcctcagttt
     4561 gagctgatgg aaagcacgcc agacgatacc tggcagccca cctcgcgcgg gcaaaaattc
     4621 ctgcaagatg acgaaaaaac gctgcgttca ctggacgacc aggaaggaat tctgcaactg
     4681 cttgagctgc tggctggccg agaaatgtcg cgccgcgccg atttattgcc ggaatggcag
     4741 gcctttttgc atcagcattc gaaattcgcg tcagcaagtt cagtgaaaag cacgctctac
     4801 tcccgtctct ataaccttat cgaccgtgac atggttaacc gtgaaggcat gagctatcgc
     4861 atcaccgata ccgggcgcgc ctggcttgga aaagcgttac cgacacgaaa aagcgatccg
     4921 cgtaaagagc tgctggaagc agtgaaacgt tacaacgagc agcaaaaaga actgttgcgc
     4981 gagcaaatca gcacgatgaa cccgtacaaa tttgagcaac tggtcggtca attactggaa
     5041 gcaatgggct acgagcaggt cgaagtgacc aaagcctctg gcgataaagg cgtggacgtg
     5101 attggtcagg tgcaggtcgg catcaccacc ataaccgagg tggtgcaggt caaaagaatg
     5161 cagaacacca tcacccgccc ttatatcgac cagttgcgcg gggcattgcc ttaccacaaa
     5221 gccattcgcg gtacactcat caccaccggg aaatttgcgg cgaaatgtgc agaagcggcg
     5281 ctctttccgg gtgccgcgcc cattacgctt atcgacggcg atcgtttact ggaactgtta
     5341 atcgaaaata acgtcggcat tcgccgcagc aatgcagttg aattgctgga tgtagatttg
     5401 cagttgtttg atgagctgga gatggattaa agggcgaaat ccgccgcctc aaccattaca
     5461 atagcgaccc aatttatctg aacagagttc atcatggcca aagcaccaac taaaaaagcc
     5521 aaagcaaaga aaggctttga agaaacatta tgggatacag caaaccagct tcgcggtagc
     5581 gttgagtcct ccgaatacaa gcacgtggtg ttgagcctcg tgttcctgaa attcatcagc
     5641 gataagtttg aagcacgccg caaaaaaatg attgccgatg ggcaagcaga cttccttgag
     5701 atggaagtgt tctaccagca ggacaacatt ttctacctgc cggaagaggc gcgctggtca
     5761 tttatcaaac aaaatgccaa acaggacgat attgcggttc gtattgacac cgccctctcg
     5821 accattgaga aacgtaaccc aaccctgaaa ggcgcgctgc cagacaacta cttcagccgt
     5881 cagaatctgg aaaccaaaaa actggcgtca ctgattgata ccatcgacaa catcgaaacg
     5941 ctggcacacg aaactgacgt tgaaacgtta tcgaaagaag acctggtcgg acgcgtttac
     6001 gaatacttcc tcggtaagtt tgccgcaacc gaaggcaaag gcggtggtga gttctacacg
     6061 cccaaatgtg tggttacgct gttaactgaa atgctcgaac ccttccaggg caaaatttat
     6121 gatccgtgct gcggctcggc ggggatgttt gtgcagtcgg tgaagtttgt tgagagccat
     6181 cagggtaaaa gtcgtgatat cgcgctgtat ggtcaggaac tgacagccac gacgtataaa
     6241 ctggcaaaaa tgaacctcgc cattcgcggt ctttcagcta acctcggcga acgaccggca
     6301 gacaccttct ttagcgacca gcacccggac ctgaaagccg actacattct ggcgaatccg
     6361 cccttcaacc tgaaagactg gcgtaatgaa gccgaattaa ccaaagaccc acgttttgct
     6421 gggtatcgta tgccgccaac cggtaacgcc aactacggct ggattttgca tatgctctcc
     6481 aagctgtcgg ctaacggcac agcaggtttt gtgctggcaa acggttcgat gagttctaac
     6541 acctgcggtg aaggcgagat ccgtgcgcag atgatcgaaa atgatctgat cgactgcatg
     6601 atcgccctac ctggacagtt gttctacacc acgcagatcc cggtgtgttt atggtttatg
     6661 accaaatcga aggctgccga cccagccaaa ggttatcgtg atcgtcaggg cgagacgctg
     6721 tttattgatg cgcgtaacct cggcaccatg attagccgca caactaaaga gttaacgacg
     6781 gaagatattg ccacaatcgc cgatacctac catgcctggc gcagcacgcc ggaagaactg
     6841 gctgcacgga ttgcgcgtgg tgacagcaag ctggaaaaat atgaagacca ggcaggcttc
     6901 tgcaaagttg cgaccctgca agatattaaa gataacgact acgtgctgac gccgggccgc
     6961 tatgtgggtg cagccgagca ggaagaggac ggcgtggcat ttgagacaaa aatgcgcgaa
     7021 ttgtcgaaga cgttgtttga gcagatgaag caggcggaag aactggatcg tgcgattcgc
     7081 aagaatctgg aggcgctggg ttatgggctt tgattttcag ttgttagaaa atgcagggat
     7141 ccagctaatt gatggtgaca gaggcaaaaa ttatcctaaa cataatgatt ttatggagaa
     7201 tggctattgt cttttcttat ccgctaaaaa tgtcactaaa tctggttttc aatttcaaga
     7261 aacattattt atcaatgaaa ctaaagatcg tgaactaaga gcaggaaagc ttaagtacgg
     7321 tgacattgtt cttactaccc gtggcacag
//
LOCUS       DADSZD010000190         7343 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.192, whole genome shotgun sequence.
ACCESSION   DADSZD010000190 DADSZD010000000
VERSION     DADSZD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.192"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..464
                     /gene="ecpR"
                     /locus_tag="J1Z28_003891"
                     /gene_synonym="matA"
     CDS             <1..464
                     /gene="ecpR"
                     /locus_tag="J1Z28_003891"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HBA7718674.1"
                     /translation="VDIDRLIYLSLEKIRKDFVFINLNTDSLTEFINRDNEWLSAVKG
                     KQVVLIAARKSEALANYWYYNSNIRGVVYAGLSRDIRKELAYVINGRFLRKDIKKDKI
                     TDREMEIIRMTAQGMLPKSIARIENCSVKTVYTHRRNAEAKLYSKLYKLVQ"
     gene            538..1125
                     /gene="ecpA"
                     /locus_tag="J1Z28_003892"
                     /gene_synonym="matB"
     CDS             538..1125
                     /gene="ecpA"
                     /locus_tag="J1Z28_003892"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HBA7718675.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1182..1850
                     /locus_tag="J1Z28_003893"
     CDS             1182..1850
                     /locus_tag="J1Z28_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HBA7718676.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIQNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            1876..4401
                     /locus_tag="J1Z28_003894"
     CDS             1876..4401
                     /locus_tag="J1Z28_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718677.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEEGEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSMSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4391..6034
                     /gene="ecpD"
                     /locus_tag="J1Z28_003895"
     CDS             4391..6034
                     /gene="ecpD"
                     /locus_tag="J1Z28_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HBA7718678.1"
                     /translation="MRVNLLIAMIIFAIIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6003..6713
                     /locus_tag="J1Z28_003896"
     CDS             6003..6713
                     /locus_tag="J1Z28_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718679.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELSTRPVDGELLFAPRQLALQTGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWF
                     FDQVTGTVSNTGNTWFKLLIKPGCDTTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7020..>7343
                     /locus_tag="J1Z28_003897"
     CDS             7020..>7343
                     /locus_tag="J1Z28_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBA7718680.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYENRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTP"
BASE COUNT         1886 a         1907 c         1928 g         1622 t
ORIGIN      
        1 ttgtggatat cgacagatta atttatctat cgctggagaa aatcagaaaa gatttcgtgt
       61 ttatcaatct caatacggat tctttaactg agtttataaa ccgtgataat gagtggttat
      121 ccgcggtaaa ggggaaacag gtcgtattga ttgcggccag aaagtcagaa gccttagcaa
      181 attattggta ttacaacagc aatattaggg gcgtggtata cgctggactg agtcgtgata
      241 ttagaaaaga actggcctat gtgattaatg gcaggttcct gagaaaagat attaagaaag
      301 ataaaatcac tgaccgggaa atggaaatta tccgcatgac ggctcaggga atgctgccta
      361 aatcgattgc cagaattgaa aattgtagtg tgaagacagt gtatacccat cggcggaatg
      421 cagaggccaa gctgtactca aaattatata agttggttca gtaaactcca ggcaagttag
      481 ttttaaaaat gactcaccgg gacttcatgt cctcaattca actcgggaag aaaagcaatg
      541 aaaaaaaagg ttctggcaat agctctggta acggtgttta ccggtacagg tgtagcgcag
      601 gctgctgacg taacagctca ggctgtagcg acctggtcag caacagccaa aaaagacacc
      661 accagtaagc tggttgtgac gccactcggt agcctggcgt tccagtatgc cgaaggcatt
      721 aaaggtttta actcacaaaa aggtctattt gacgtggcta tcgagggtga ctcaacggct
      781 accgccttta aactgacctc acgccttatc accaacacct taacccagtt ggatacctca
      841 ggttccacac tgaatgtggg cgtggattat aacggcgcgg cagtcgaaaa aactggcgat
      901 accgtgatga tcgataccgc caacggcgta ctgggcggca accttagccc actggctaac
      961 ggttacaatg ccagcaatcg taccaccgca caggatggtt tcactttctc catcatcagc
     1021 ggcaccacca atggtaccac cgcagtaacc gattacagca ctctaccgga aggcatctgg
     1081 agcggcgacg ttagcgtaca gttcgacgct acctggacca gttaatctct ctgatgtacc
     1141 agcaggggta cccccctgct ttattcctgg acggaattgt tatgaaaaag caccttctgc
     1201 ctctcgctct gctgttttcc ggaatatcac cggcccaggc gctggatgtc ggcgatatat
     1261 catcgtttat gaacagtgac agcagcacgc tgagcaaaac gatccaaaac agtaccgaca
     1321 gtggccgcct catcaatatc cgtctcgaac ggctctcttc accgcttgac gacgggcagg
     1381 ttatcgcaat ggacaagccg gatgagttgc tactcactcc cgccagcttg ctgctacccg
     1441 cccaagccag cgaagtgatc cgcttcttct ataagggacc agcagatgaa aaagagcgct
     1501 actaccgcat tgtctggttt gatcaggccc tcagtgatgc gcagcgcgat aatgccaacc
     1561 gtagcgctgt ggccactgct tccgcccgca tcggcaccat tctggtcgtc gcaccccgcc
     1621 aggcgaacta ccactttcag tacgccaacg gctccctgac aaatacagga aatgcgacgt
     1681 tgcggatcct cgcctacggc ccctgcctga aagccgccaa tggtaaggag tgtaaagaga
     1741 attactacct gatgccgggc aagtcgcgtc gttttacccg cgtggacacg gcggataaca
     1801 aaggacgggt tgcactttgg cagggtgata agttcattcc cgtgaaatag atagctgtgc
     1861 agatggataa cgacaatgcc tttacgacgg ttctccccag gactgaaagc ccagtttgcc
     1921 ttcggcatgg tctttttgtt cgttcagccc gatgccagcg ctgctgacat aagtgcgcag
     1981 caaataggtg gggtgattat tccgcaggcc ttcagtcagg cgcttcagga cggcatgagc
     2041 gtcccgctct atattcatct cgccggtagc cagggtcgcc aggacgatca gcgaatcggc
     2101 agcgctttta tctggttgga tgatggacag ttacgcatcc ggaaaataca gctggaagag
     2161 ggtgaagata acgccagtgt cagcgaacaa actcgacagc aactgatgac tctggccaac
     2221 gccccgttca atgaggccct taccatcccc ctgactgaca acgcgcagtt ggatctcagc
     2281 ttgcgccaac tgctgctgca gctggtggtc aagcgcgaag cgctgggcac cgtactacgc
     2341 tcacgtagcg aagacatcgg gcagtccagt gttaacaccc tcagcagtaa tctgagctat
     2401 aacttcggcg tctataacaa ccagttgcgt aacggcggga gcaacacctc cagctatctg
     2461 tcgctgaata acgttactgc gctgcgcgaa catcacgtgg tgctcgacgg ctcactgtac
     2521 gggattggta gcggtcaaca ggacagtgaa ttatataaag cgatgtatga acgcgatttt
     2581 gctggtcacc gatttgccgg tggaatgctc gacacctgga acttgcagtc cttagggccg
     2641 atgaccgcca tttcagcagg gaagatttac ggcctttcct ggggaaacca ggccagctcc
     2701 accatcttcg acagcagcca atcggccacg ccagtgatcg cctttttacc ggcggcgggc
     2761 gaagtacatc tcacccgtga tgggcgacta ctaagcgttc agaacttcac catgggcaat
     2821 catgaagtgg atacccgggg tctaccgtac ggtatttacg atgtggaagt tgaggtaatc
     2881 gttaacggtc gcgtgatcag caaacgcacc cagcgggtca ataagctgtt tagccggggg
     2941 cgtggcgtcg gtgcaccgct ggcgtggcag atatggggcg gtagctttca tatggatcgc
     3001 tggtcggaaa acgggaaaaa gacgcgacca gccaaagaga gttggctggc aggtgcctcg
     3061 acctccggct caatgagtac gtttagctgg gcggcaacgg gatatggata cgataatcag
     3121 gcggtgggtg aaacccgtct gacgctgccg cttggggggg cgatcaacgt taacctgcaa
     3181 aacatgctgg ccagtgacag ctcatggagc aacatcgcca gcatcagcgc cactctacct
     3241 ggaggcttca gttcgctgtg ggttaaccag gaaaaaaccc gcattggcaa tcaattgcga
     3301 cgtagcgatg ccgacaaccg tgcaatcggc ggcacactca acctgaactc actgtggtcg
     3361 aagctgggta cgttcagcat cagctacaat gatgaccgcc gctacaacag ccattattac
     3421 acggcagatt actatcaaag tgtctacagc ggtacctttg gttcgcttgg cctgcgggcc
     3481 ggtattcagc gctataacaa cggcgacagc agcgccaata cagggaaata tatcgctctc
     3541 gatctctcgc taccactggg caactggttt agcgcaggga tgacccatca aaacggctac
     3601 accatggcaa acctgtcagc acgcaaacag tttgatgaag gaaccattcg cactgttggt
     3661 gccaatctgt cacgagccat ctccggcgat accggtgatg acaaaaccct cagcggtggg
     3721 gcgtatgcac agttcgacgc tcgttacgcc agcggaacgc tgaacgtcaa tagcgcggcg
     3781 gacggctaca tcaataccaa tttgaccgcc aacggcagcg tcggctggca gggtaaaaac
     3841 atcgctgcca gcgggcggac cgatggcaac gctggggtga tattcgacac tggcctggag
     3901 aacgatggtc agatcagcgc caaaatcaac gggcggattt tcccgcttaa cggcaagcgt
     3961 aactatctcc cgctctctcc ctatggaaga tatgaggtgg agttgcagaa cagcaaaaac
     4021 tcactcgaca gttacgatat cgtcagcggt cgcaaaagtc atctgactct ctatccaggc
     4081 aatgtcgctg tcattgagcc agaggtgaag cagatggtta ccgtctccgg tcgtatccgt
     4141 gcggaagacg gcacactgct ggctaacgca cggattaaca accatatcgg ccgaacccga
     4201 accgatgaaa acggcgagtt tgtcatggac gtggataaga aataccccac tatcgatttt
     4261 cgctacagtg gcaataaaac ctgcgaagtg gcactggaac tcaaccaggc gcgcggtgcc
     4321 gtctgggtcg gtgatgtggt ctgcagcggc ctctcatcgt gggcggcggt gacgcagaca
     4381 ggagaagaga atgagagtta acctactgat agcgatgata atctttgcga taatctggcc
     4441 tgcaaccgcg ctcagagcgg cagtgagcaa aacaacctgg gcggatgctc cggcacgcga
     4501 gtttgtgttt gtcgaaaaca actcagacga caactttttc gtcactcctg gcggggcgct
     4561 ggatccgcgc ctgaccggtg ccaaccgctg gaccggttta aaatacaatg gttcaggaac
     4621 catctatcag caaagcctcg gctacattga taacggttac aacaccggcc tttataccaa
     4681 ctggaagttt gatatgtggc tggaaaattc accagtttca tctcctttaa ctggcttgcg
     4741 ctgcatcaac tggtacgcag ggtgtaatat gaccaccagt cttatcctgc cgcaaaccac
     4801 cgacgccagt ggattttatg gcgcgacggt gaccagcggc ggcgcgaaat ggatgcacgg
     4861 catgttgtca gacgcgtttt accagtatct gcaacaaatg cccgtcggca gcagctttac
     4921 aatgaccatc aatgcctgcc agacctctgt gaactatgac gccagcagcg gcgcacgctg
     4981 taaggatcag gcctccggca actggtatgt tcgcaacgtc acccatacga aagcagcaaa
     5041 tctgcggttg ataaataccc actcgctggc ggaagtattt atcaacagcg acggagtacc
     5101 gactctgggc gaagggaacg ccgactgccg gacgcaaacc atcggcagcc gttcaggatt
     5161 aagttgtaag atggttaact ataccctgca aacaaacgga ctcagcaaca cctcaatcca
     5221 tatattcccg gcgatcgcca actcgtcgtt agcctcggcc gtcggggcgt acgatatgca
     5281 gttcagtctg aatggcagtt catggaaacc ggtgagcaat actgcctatt actacacctt
     5341 caacgagatg aagagcgcag actcgatcta tgttttcttc tcgagcaact tctttaaaca
     5401 gatggtgaac ctcgggatca gcgatatcaa caccaaagat ctattcaact ttcgctttca
     5461 gaacaccaca tcaccggagt ctggctggta tgaattttct acctccaaca cgctgattat
     5521 caaaccccgt gatttcagca tcagtattat ctccgatgaa tatactcaga caccgtcgcg
     5581 ggagggatat gttggcagcg gcgagtcggc actcgatttc gggtatatcg taaccaccag
     5641 cggtaaaaca gctgccgacg aagtgctgat caaggtgacc ggacccgcgc aggtgattgg
     5701 cgggcgctcc tattgtgtct tcagctccga tgacggtaag gcgaaagtac cgttcccggc
     5761 gacgctttcc tttattaccc gcaacggagc tacaaaaacc tacgatgccg ggtgcgatga
     5821 tagctggcgg gatatgaccg atgcgctgtg gttgaccaca ccgtggactg atatctctgg
     5881 cgaagtgggg cagatggata agaccacagt caaattttcg attccaatgg ataacgccat
     5941 ttctctgcgt acggtagatg ataacggctg gtttggcgaa gtcagcgctt caggagagat
     6001 tcatgttcag gcgacgtggc gtaacattaa ctaaggccct gctgacagtg gtctgtatgc
     6061 tggcggcacc tttgacacag gcgatttcgg tcggcaatct gactttttca ctgccgtccg
     6121 agactgactt tgtcagcaaa cgtgtcgtga ataacaacaa aagcgcgcgg atataccgta
     6181 ttgccatcag tgctattgat agcccgggca gcagtgaatt gagcacccga ccggtggatg
     6241 gtgaactact tttcgccccc cgccagctgg cgttgcagac tggtgagagc gagtatttta
     6301 aattttacta tcatggtcca cgggataacc gcgagcgcta ctaccgggtc tcatttcgcg
     6361 aggtccccac tcgtaacctg acaaaacgca gcccaaccgg cggtgaggtc agcacggagc
     6421 cggtggtggt gatggatacc attctggtag tacgaccacg tcaggttcag tttaaatggt
     6481 tcttcgatca ggtgaccgga acggtaagta acaccggcaa cacgtggttt aagctactga
     6541 ttaaaccagg atgtgatacg accgaagagg aaggcgatgc ctggtatcta cgtccgggag
     6601 acgtggttca tcagcctgag ttacgtcagc cggggaatca ttatctggtc tataacgaca
     6661 aattcattaa gattagcgat tcctgtccgg ctaagccccc ttcggcggac taagacttat
     6721 ccatcagacg agggaagaat tccacctcag agactcaaaa atcgtttaaa tgatgtggaa
     6781 gcgatcgtca atgtcaccga tgagctttct catatgcaca ccacagtgtt caggagacac
     6841 ggaaaggtac caggcggcct gctaacgagt ggtttcttca ctaattagga cgtaaaatcg
     6901 gtaacggctg gaaatcattc aatactcaca ctatcgaaag tacaccagcc aaccgcgata
     6961 cgtccctgca tacgacgtac cgcagttctc tttacgattt atagctgtac tggtgaatta
     7021 tgagcaatct gaatccatgc atgacgtgtg gtgcctgttg tgcatttttc cgcgtctctt
     7081 tttactgggc cgaagccgac gatgctggcg gtaccattcc cgccaggctc actgaacaaa
     7141 tatccccttt tcaccgatgc atgagcggta ctaatcagaa aaatccccga tgtattgccc
     7201 ttgcaggaac accgggcaaa aatgcctgct gcacgatata tgaaaatcga tcgtccacat
     7261 gcagagaatt cgccatgtct ggtgagaacg gagaagtcaa tgaggcttgc aatcgtgcaa
     7321 gagctaaata cgggctaaca ccg
//
LOCUS       DADSZD010000191         7228 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.193, whole genome shotgun sequence.
ACCESSION   DADSZD010000191 DADSZD010000000
VERSION     DADSZD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.193"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            82..213
                     /locus_tag="J1Z28_003898"
     ncRNA           82..213
                     /ncRNA_class="other"
                     /locus_tag="J1Z28_003898"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(397..1074)
                     /gene="narI"
                     /locus_tag="J1Z28_003899"
     CDS             complement(397..1074)
                     /gene="narI"
                     /locus_tag="J1Z28_003899"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBA7718681.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(1074..1784)
                     /gene="narJ"
                     /locus_tag="J1Z28_003900"
     CDS             complement(1074..1784)
                     /gene="narJ"
                     /locus_tag="J1Z28_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBA7718682.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1781..3319)
                     /gene="narH"
                     /locus_tag="J1Z28_003901"
     CDS             complement(1781..3319)
                     /gene="narH"
                     /locus_tag="J1Z28_003901"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBA7718683.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3316..7059)
                     /locus_tag="J1Z28_003902"
     CDS             complement(3316..7059)
                     /locus_tag="J1Z28_003902"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBA7718684.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         1600 a         2053 c         1882 g         1693 t
ORIGIN      
        1 cgacttcgaa ggttcgaatc cttcccccac caccatcact ttcaaaagcc cctgaattct
       61 caaacgaatc cgcaattaaa tattctgccc atgcggggaa ggatgagagg cttcgaccaa
      121 ggttcgactc gagtgccagc gagagagcgt tgccgcaggc aacgacccgt agggcgaagc
      181 gcgcagcgct gagtaatcct tcccccacca ccatttcttc ctgaaatact caaaacctgt
      241 tactccgagc taattctgcc caatggcacc gcgtttaccg gacctaaaat atcctcccct
      301 gaagttcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
      361 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
      421 atacttacgc gtcagatact ccaccgggac gctccagatg tgtaccagac gcgagaacgg
      481 gaacagcagg aataacgtca tccccaacac caggtgcaga cggaagataa acgccacgcc
      541 atcaagatgt tgagaagcgc caccgtggaa ggtcactacc gactgcgccc agccaaccag
      601 tttcatcatc tcgctaccgt ccatatgctg ggcggagaac ggaatggtca gcaggcccag
      661 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tcgttgcacg
      721 cacgcgtggg ctgaacagac gacgtttcaa caacagcacg ccgccaatca gacacagtac
      781 gccgctggca ccaccagcaa acatcgccat tttctgtttt acttcaatcg gcagccaggc
      841 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccgacaaaaa tccccagaat
      901 accgatatgg aacaggttcg acgcgaggtt catccctttg cgatccagca tctggctgga
      961 cgccgcacgc catgtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     1021 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     1081 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     1141 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     1201 tcccagactg catccagcgc ctgcggcgta tcgtcgcgcg cttcgtcggc aattttttcc
     1261 gccactttgt cgctgtcgat tgcggtattc gccagtttca gcagcagatc aaacagcacg
     1321 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcaata
     1381 tctttcaaac cctccacggc ttcgctttgc ggcagctgcg ccaggtactc cagatacagc
     1441 ggcagatggt cgggcaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     1501 agcagatcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     1561 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgcgcctg ggcatcgagc
     1621 ggatccatcg tcgttaaatc gcgcaggaaa atgcccagcg catgggcatc ctcttttggc
     1681 agatttttcg acgcggcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     1741 tccggatatt caaggagacg cgatacaatc acgagttcga tcatgggtgc ggctccgttt
     1801 tgctggttac gtcgatggca tcgatacggc ggctgttgaa cagattgaat ttggtatctg
     1861 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     1921 gcgccagttc acgatgactg ctcggcacca caaagcgatc ttcgtagttg gcaatagcca
     1981 gataacgata catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     2041 tatcgacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     2101 tcagcgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     2161 ccggaatacg caggctttcg acgtctggca gaatgccgtt gctgcccagt tcacccgcgt
     2221 ctgccgcaga ctgaatcggc gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     2281 attccggatg cagcggcagc gccagtttcc attccatcgc cattttataa actggcgact
     2341 gctgtgcagc ctcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     2401 tcggatcgtt cggatcgagg aacacatcca gctgacgctg gtagagatct ttctcgttct
     2461 cggtacttgc cgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     2521 tacgaccgac acaggtttct gaacatattg tcggctggcc ggcttcaata cgcggatagc
     2581 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     2641 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     2701 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcagac
     2761 agtgttcgca caggcgcggc aaatacatca tgaaggtgtt ttcgaactgg ctatacatcg
     2821 ccttctggat gttgtcgaag ttcttgtctt tcgccagctt gtcaaactca ccgcccaggt
     2881 catcttccca gttcggccct ttttcgattt tcgccatccg ttcgccggta atcagtgaac
     2941 gcggacgggc aatcggctgt gatttgctgc cttccggcgc ggtatgcagg ttctgataat
     3001 caaaatcgaa tggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     3061 tacccagcag catggcacgg ttacccatgc gcggctgaag tttgccgttg attttacgga
     3121 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tggcccggct
     3181 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
     3241 tacaggtcac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
     3301 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
     3361 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
     3421 gccgtagtag ttaaagccat atgccagatg ggcatagccg ccgatcatat gcgtcggttt
     3481 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
     3541 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
     3601 ctggctgaca accgcacggg cagtcagtgc accgttgctg ttgaagactt caatccagtc
     3661 gttatcggcg atacccagct ctttggcatc ggcttcactc agccagacca ccggaccacc
     3721 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
     3781 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct gccccatcac
     3841 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
     3901 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccacgggat
     3961 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt caaggccaga
     4021 ccatgtcggg ctggagatga ttttgcgcgg ctgtgcctga atatcgcgga agcgaatctt
     4081 ctcgtcttct ttattcagcg ccagatgcgt atggtcacgg ccggtaaact cgctcagggc
     4141 agcccaggct tttacagcta cctgaccatt ggtttccggt gccagcgtca ggatcatctc
     4201 tgccgcatca attgcggtat tgagcatcgg ctggcctttt gccggacctt ccgctttggt
     4261 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
     4321 gccgttaccg attttctcca tcagcgggcc gatagaggta aagcgttcgt aagttgctgg
     4381 ataatcgcgc tctacgacca taatgtgcgg tgcagtttta cctgggatca ggtcgcattc
     4441 gccttttttc cagtctttca catccagcgg ttgcgccagt tcagcggcag agtcgtgctg
     4501 gataggcagc gtgacgatgt cagtttcttt acccagatga ccaacgcaca cttcggagaa
     4561 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
     4621 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
     4681 gtaccaggtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
     4741 gtccagcgtc accaccagat ccagcttgcc ttccagaccg ttgtcctgcc agtccacttc
     4801 ttccggcttc acgccgccct gctgccccag gtctttgccc tggataccgt gctccgtccc
     4861 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
     4921 ccagatgaac agattacgcg ggtggttttt accgttttcc ggctgttccg cagcgaaacg
     4981 gatagaaccc tctttcaggg atttaaccgt atagtccacc ggattcatcc cagctttttc
     5041 tgcttcgcga gcgatagtca gcgggttagt gcctaactgc ggcgcagacg gcagccagcc
     5101 catgcgttct gcacggacgt taaagtcgat caggtgtcca gtatagcgag atttatccgc
     5161 catcggcgac agcagttctt ccgcagtcac ggtttcataa cgccactggc tggagtggtt
     5221 atagaaataa gaggtactgt tcatgtgacg cgccggacgc tgccagtcga gggcaaacgc
     5281 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
     5341 accgccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
     5401 catgtccagg tgataccagt ggttcagccc agcgccgacg ataatcatcg aacgaccgtg
     5461 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
     5521 gccggtaatc tgctcggccc aggcaggggt gtaagctttc acatcgtcat agctggttgc
     5581 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
     5641 ggtggtcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagcttatg
     5701 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccatcgc caccaaagta
     5761 cgggaagccc acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
     5821 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
     5881 gaagccaata gaaccgttcg gcgcaaccat ttcaccattg gtattaaagg cgacagtttt
     5941 ccattccgga ttgttttcct ggcccagtgc atccaccaga tcagcagcgc gcagcatacg
     6001 acccgcagcg tagtaaccgt cgcgctcttc cagcatcacc agcatcggca tgtcggtgta
     6061 gcggcgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
     6121 tacgtgaccc attgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
     6181 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtcccttt
     6241 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
     6301 ccaggcgatg atgtagctgg agttgtacca gtcagcagat tccggtacgt cagtttgctc
     6361 accccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
     6421 agtaccgcca atcagcgaga gatagcgcgc acccgatgcg taagaaacca tcgacattgc
     6481 cggaattggc gagaagccag cgacacggtc cgggccgtaa tttttgatgg tgtaaacgtt
     6541 agatgcggcg atcagttcgt tcacttcctg ccaggaagaa cgaacaaaac caccgcgtcc
     6601 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
     6661 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
     6721 catcgggtat ttcaggcggt tagcgctgta aagataccag gagtagctgg caccgcgcgg
     6781 gcagccgcga ggttcatggt ttggcaggtc cgggcgagtt cgcggatagt cagtctgctg
     6841 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
     6901 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
     6961 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
     7021 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
     7081 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
     7141 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctgga
     7201 ataccccttt aggataatta acatttat
//
LOCUS       DADSZD010000192         7199 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.194, whole genome shotgun sequence.
ACCESSION   DADSZD010000192 DADSZD010000000
VERSION     DADSZD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.194"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            87..1370
                     /locus_tag="J1Z28_003903"
     CDS             87..1370
                     /locus_tag="J1Z28_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBA7718685.1"
                     /translation="MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIV
                     GAFIFGKMGDRIGRKKVLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGL
                     GAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLA
                     WGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLA
                     TGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKI
                     GRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMA
                     EMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLIS
                     ALKMPEVKDRDLSALEDAAEDQPHVVRAAQPSRSL"
     gene            1459..2919
                     /locus_tag="J1Z28_003904"
     CDS             1459..2919
                     /locus_tag="J1Z28_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBA7718686.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     MIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAIDVKLAQWIEDNVTFS
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2955..3158)
                     /gene="ydfZ"
                     /locus_tag="J1Z28_003905"
     CDS             complement(2955..3158)
                     /gene="ydfZ"
                     /locus_tag="J1Z28_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBA7718687.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3335..4021)
                     /locus_tag="J1Z28_003906"
     CDS             complement(3335..4021)
                     /locus_tag="J1Z28_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7718688.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4110..4856)
                     /gene="ydfG"
                     /locus_tag="J1Z28_003907"
     CDS             complement(4110..4856)
                     /gene="ydfG"
                     /locus_tag="J1Z28_003907"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBA7718689.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     EDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4993..7038
                     /gene="dcp"
                     /locus_tag="J1Z28_003908"
     CDS             4993..7038
                     /gene="dcp"
                     /locus_tag="J1Z28_003908"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBA7718690.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIVLAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELTSIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
BASE COUNT         1801 a         1671 c         1840 g         1887 t
ORIGIN      
        1 aggatgcttt ttaaaaaatg caactatctg aaaaaaccta gaaaacgcca aggaaaccac
       61 aggatgggaa aaaacacctg tgaattatgg acttccagtt atattcgctc ggcgcagcgt
      121 tagtgtttca tgaaatattt tttcctgaat catcaacggc aatggcgtta attctggcaa
      181 tgggaaccta tggtgcaggt tatgtggcac gtattgtcgg agcatttatt ttcggcaaaa
      241 tgggcgacag aatagggcgt aaaaaagtgc tctttattac catcaccatg atggggatct
      301 gtaccacctt aattggtgtg ttaccgactt atgcacaaat tggtgttttt gcccccatct
      361 tgctggtgac gctgcgtatt attcaggggt tgggtgcagg tgcggaaatt tccggtgccg
      421 gtacgatgct ggcggaatat gcgccaaaag gtaagcgcgg aattatctcc tcatttgtgg
      481 ctatgggaac taactgcgga accttgagcg caacggcaat ctgggccttt atgttcttca
      541 ttctcagtaa agaggaactg ctggcgtggg gatggcgtat acctttcctg gcgagcgttg
      601 tcgtgatggt ctttgctatc tggttgcgta tgaatctgaa agaaagcccg gtctttgaga
      661 aggttaacga cagcaaccaa ccgacagcaa aacctgcacc tgctggtagc atgttccaga
      721 gcaaatcctt ctggctggca acagggctgc gttttggtca ggctggtaac tcaggtttaa
      781 ttcagacttt ccttgcaggc tatttagtgc agacgttatt gtttaacaaa gcaattccaa
      841 cagatgcatt gatgatcagt tcgattctcg gctttatgac cattccgttc cttggttggt
      901 tatccgataa aattggtcgc cggatcccgt atattattat gaatacctct gcgattgtgc
      961 tggcatggcc aatgctttct atcatcgtag ataaaagcta tgccccgagc accattatgg
     1021 ttgcactgat tgtgattcat aactgtgcgg tgcttggatt atttgctctg gaaaacatta
     1081 ccatggcaga aatgttcggc tgtaaaaacc gctttacccg aatggctatt tctaaagaaa
     1141 ttggtggtct tatcgcttcc ggttttggtc ctatcctggc ggggattttc tgcaccatga
     1201 cggaatcctg gtatccgatc gccattatga tcatggcata ttctgtgatt ggtttaatct
     1261 ctgcgctgaa aatgccagaa gtgaaagacc gtgatttaag tgcgctggaa gacgccgcag
     1321 aagatcaacc acatgttgta agagctgcgc aaccgtccag aagtctgtaa atcctcaatc
     1381 ccttctctta ccggagaggg gatttttatt catataacaa aacatgtagc ttgctatatt
     1441 taattttaag gaattgttat gggaaatcat ttgttatcag caaaagcgac gctccctgtt
     1501 tatgatcgta ataacctggc cccaagaatt gttcatttag gctttggtgc atttcaccgt
     1561 gcgcatcagg gcgtgtatgc cgatattctt gctacggaac atttcagtga ctggggatat
     1621 tatgaggtca acttaatcgg cggcgaacag caaattgccg atttacaaca gcaagataat
     1681 ctttataccg ttgcggaaat gtcggctgat gcgtggacgg ctcgcgtcgt tggcgtcgtt
     1741 aaaaaagcct tgcacgtaca aatagatggc ttagaaaccg tgttggctgc gatgtgtgaa
     1801 ccgcaaatcg cgattgtctc tctgacaatc accgaaaaag ggtatttcca ctctccggcg
     1861 accggacagt taatgcttga tcacccgatg gtcgttgccg acgtgcaaaa tccccaccag
     1921 ccgaaaactg caacagggat gattgttgag gcgctggctc gccgtaaagc ggcaggactt
     1981 cccgcattta ccgtcatgtc atgtgacaac atgccagaaa acggtcatgt tatgcgtgac
     2041 gttgtcactt cctatgcgca agccattgat gtaaaactgg cacaatggat cgaagataac
     2101 gtgactttct catcaacaat ggtggaccgt attgtgcccg cagtgacaga ggatacgctg
     2161 gcgaaaatcg aacaacttac cggtgtgcgc gatccggcag gcgttgcctg tgaacctttc
     2221 cgccagtggg taattgaaga taactttgtt gccggacgtc cggaatggga aaaagcggga
     2281 gccgaactgg ttagtgatgt gctgccttat gaagagatga agttgcgcat gctcaatggc
     2341 agtcattcat tcctggcgta tctggggtat cttgccggat atcagcacat taatgactgc
     2401 atggaagatg aacattatcg tcatgcagcg tatgccttaa tgttgcagga acaagcgccg
     2461 acgctgaaag tgcagggcgt tgatttgcaa gattacgcta accgattaat tgaacgctat
     2521 agcaacccgg cgttacgtca tcgaacctgg cagattgcga tggatggcag ccagaaattg
     2581 ccacagcgga tgttggattc tgttcgctgg catctggcgc atgacagcaa gttcgatctg
     2641 ctggcgctgg gcgtcgcggg ttggatgcgt tatgtcggtg gtgttgatga acagggaaat
     2701 ccgatagaaa tcagtgatcc actgttacct gttattcaga aggcagtaca aagtagtgct
     2761 gaagggacag cgcgcgtcca gtcattgctg gcaattaagg cgatctttgg tgatgattta
     2821 ccaggcaata gtttgtttac gacaaaagta acggaagcgt acttgtcttt attagcgcat
     2881 ggcgcgaaag cgaccgtggc gaaatattcc gtgaagtaaa aaaagcaatg ccgccatcgg
     2941 cggcattcac acgcttagtt catgccgaga cgaatcaggt ccagtggtgc cagtttctct
     3001 tcacaacctt caacaactac ggtttttccg cggcggattt gctcagcggt cagaccttca
     3061 gtatcaatcg acaggatctt gccagtgtga ccggtgccgc taaccataac acggctgcca
     3121 gtggtgattg cgttacggtt acgatcgtag gtcgtcatag gtgttttctc ctttctgatt
     3181 tacagtcgaa gcccacgtaa cgggcgcaat ataaatactc ctgtcgagtg aaatattttt
     3241 tgtttttgat caagctcaca tcttttttga tgcatgcatg agccataagg cgggggaaaa
     3301 gactctcacc ccggatggga tgagaggaaa tgaattactc ttcgctaaac cagtcgctgt
     3361 tttcctggcg gatctggcgc acagattcgc tgatttcctg caaatgttgc gtcattgcac
     3421 gttctaccgc atcgccatca cgtttttgca gggcagagaa aatatcaaga tgctggcgta
     3481 acagcatttc tggtggagaa acgtggtcga aactcatata gcgcacgcga tcaacggtcg
     3541 ctttcaggtt ctcaatggta tcccacgcca gttgacagtc ggcaatctgc gtcaggagtt
     3601 gatggaagtt gtcatcaagt tcaaaaaaat catccagttg cttgcgctca atggcaatgc
     3661 gttgctggtg aagattttgt tccagttggt agcactggct ttcggtaatc atgctcgccg
     3721 cccgacgcgc caccgcgcac tcaatggcct gacggataaa actgccgttg cgcacctggg
     3781 ccatggaaat tttgttgacg tagctgccac gctgcggacg aatttgaatc agaccgtttt
     3841 ccgccagttt aataaaggct tcacgaaccg gctggcgtga cacattgaaa cgaacagaaa
     3901 cttctttttc cgacaacggt gtgcctggtg caatcaggca atggacaatg tcgcggcgaa
     3961 gaatacgata aatctgctga ttgacaggct gtgtgggatt aagttgcgtt tcgacggtca
     4021 ttcgttcttg cttgtgagtg agttaactgc atgagtctac cacttttttg tgacaagcaa
     4081 taacccggca gttacgccgg gtataaaaat tactgacggt ggacattcag tccggcatag
     4141 ctttgggtaa ccggcatcat ttccagggta ttgatattga cgtgagcagg cagcgttgac
     4201 acccaccaga cggcttcgct gacatcttct ggtgtcaatg caacggtatt ttgataggtt
     4261 ttttccgctt taccgtcatc gcctttaaag cggacattgg aaaactcggt gccacccacc
     4321 agacccggtt cgatgtcggt gacgcgcacc gccgtaccat gcaggtccgt acgcagattc
     4381 aggctaaatt gacgaacaaa cgctttcgtc gcaccgtaaa cgttaccacc ggcatacggc
     4441 cagctacctg ccgttgagcc aatgttaata atatgaccat gattacgttc aaccataccc
     4501 ggtaagacgg cgcgcgtcat atataccagg cctttgttgt tggtatcaat catcgtttcc
     4561 cagtcttcaa tgctggcttt atgcgcaggc tccatgccca acgccaggcc ggcattgttt
     4621 accaggatat caatattgct ccactcggca gggagcgagg ccagcatctc ttcaatagcg
     4681 gcgcggttgc gaacgtccag ttgggcgata tacagattat cttccagttc gtcttttaac
     4741 tcctgcaacc gctcctggcg acggccagtg gcgataactt tatgcccttg ttgaataaaa
     4801 cgacgagtaa tgcattcacc aaaacctgcc gttgctccag ttactaaaac gatcatctca
     4861 ctgttcctca acgcttttgt gtgtcaataa catagcacgt gatgcttaag agcgggtaat
     4921 actggtttgc aagattgcac tccgttatca gtaagcctac gctatggaac acctcgcgtt
     4981 caggagaaga aaatgacaac aatgaatcct ttccttgtgc aaagcacact gccgtatctg
     5041 gctccccatt ttgatcaaat tgccaaccat cactatcgcc cggcattcga tgagggaata
     5101 cagcaaaagc gggcagaaat tgctgccatc gcgcttaacc cgcaaacacc tgatttcaac
     5161 aatactattc tggcactgga acaaagcgga gaattactta cccgcgttac cagcgtcttt
     5221 tttgcgatga ctgcggcgca taccaatgat gaattacagc gtcttgatga acagttttcc
     5281 gctgaactgg cggaacttgc gaatgatatc tatctgaacg gggaattatt cgcgcgggta
     5341 gatgctgtct ggcaacgccg tgaatccctg gggcttgata gtgaatccat ccgcctggtg
     5401 gaggtgattc atcaacgttt tgtccttgcc ggagccaaac ttgagcaagc tgataaagca
     5461 aaattaaaag tactgaatac agaagctgcg accctgacca gccagtttaa ccagcggtta
     5521 ctggcagcaa ataaatccgg cggtctggtt gtgaacgata tcgcgcagct ggcaggaatg
     5581 agtgaacaag agattgtgct ggcggcagag gcggctcgcg agaaaggtct ggataacaac
     5641 tggctgattc cgctgctgaa taccacccag caaccagcgc ttgctgaact gcgcgatcgc
     5701 gcgacgcgtg aaaaactgtt tactgcgggc tggacgcgag cggaaaaaaa tgatgccaat
     5761 gatacccgcg ctatcattca acgtctggtg gagatccgcg cgcagcaggc gaaactgctt
     5821 ggttttcctc attatgccgc atggaaaatc gccgatcaga tggcaaaaac gccagaagca
     5881 gcgctcaact ttatgcggga aattgttcca gcggcgcgtc aacgtgcgag cgatgaatta
     5941 acctccatac aggcggttat cgataagcag cagggcgggt ttagcgcgca gccgtgggac
     6001 tgggcatttt atgccgagca ggtaagacgt gagaaattcg atctcgatga agcgcagctc
     6061 aagccttatt tcgaattaaa cacggtgttg aatgaaggcg tattctggac tgcgaatcag
     6121 ctcttcggta ttaagtttgt cgaacgtttt gatattcctg tctaccatcc tgacgttcgt
     6181 gtgtgggaaa tttttgatca taatggcgtg gggctggcgt tattttacgg tgatttcttc
     6241 gcccgtgatt caaaaagcgg cggtgcatgg atgggcaatt ttgttgagca atcaacgctg
     6301 aatgaaacgc acccggtaat ttataacgtc tgcaattatc agaaacccgc tgccggtgag
     6361 cctgcgttgt tactctggga tgatgtaata accttattcc atgaatttgg tcatacgctg
     6421 cacggccttt ttgcccgcca gcgttatgcc acgctttccg gcaccaacac gccgcgtgat
     6481 tttgtcgaat ttccgtcgca aatcaacgaa cactgggcaa cacatccgca ggtatttgct
     6541 cgctacgccc ggcattatca gagcggggcc gcaatgcctg acgaacttca acagaaaatg
     6601 cgtaatgcca gtctgttcaa taaagggtat gagatgagcg aactgcttag cgccgcactt
     6661 ctcgatatgc gctggcattg cctggaagaa aacgaagcaa tgcaggacgt cgatgatttc
     6721 gaattgcggg cgctggtggc ggaaaatatg gatcttcctg ctataccgcc acgctatcgc
     6781 agcagttatt tcgcccatat ttttggtggc ggatatgccg caggttatta cgcttatctg
     6841 tggacgcaaa tgttggccga tgatggttac cagtggtttg ttgagcaggg cggattaacg
     6901 cgtgaaaatg ggcggcgttt tcgcgaggcg attctttcca gaggtaacag cgaagatctg
     6961 gaacgcctgt atcgacaatg gcgcggtaag gcacctcaga ttatgccgat gctgcaacat
     7021 cgtggcttga acgtataaac accaccaatg gctggcctgg atttatcgcc agaccagctt
     7081 cgttatagca gtaattgcag caactgggcg agggcaaact tcaccgctaa tgcgaatgcc
     7141 gtttcgcagt cgccagcaaa attacttttt ttgtatactc ttccgttata ataattttc
//
LOCUS       DADSZD010000193         7171 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.195, whole genome shotgun sequence.
ACCESSION   DADSZD010000193 DADSZD010000000
VERSION     DADSZD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.195"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            148..579
                     /gene="osmC"
                     /locus_tag="J1Z28_003909"
     CDS             148..579
                     /gene="osmC"
                     /locus_tag="J1Z28_003909"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBA7718691.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(768..3167)
                     /gene="dosP"
                     /locus_tag="J1Z28_003910"
                     /gene_synonym="pdeO"
     CDS             complement(768..3167)
                     /gene="dosP"
                     /locus_tag="J1Z28_003910"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBA7718692.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGASFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDEGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGIETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(3192..3410)
                     /locus_tag="J1Z28_003911"
     CDS             complement(3192..3410)
                     /locus_tag="J1Z28_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBA7718693.1"
                     /translation="MCLVDFGLRFNHKGRHYFKAANGEDIALSLSIGAAMFNGHPDYE
                     RLIQIADEALYIAKRRGRNRVELCKASL"
     gene            complement(3734..5053)
                     /locus_tag="J1Z28_003912"
     CDS             complement(3734..5053)
                     /locus_tag="J1Z28_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HBA7718694.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(5184..6719)
                     /gene="gadC"
                     /locus_tag="J1Z28_003913"
     CDS             complement(5184..6719)
                     /gene="gadC"
                     /locus_tag="J1Z28_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBA7718695.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            complement(6875..>7171)
                     /locus_tag="J1Z28_003914"
     CDS             complement(6875..>7171)
                     /locus_tag="J1Z28_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA7718696.1"
                     /translation="GRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLG
                     GEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT         1844 a         1741 c         1779 g         1807 t
ORIGIN      
        1 tatatatttc cggttatcta ttgtggaaat ttaatttaag tgcaggaatt atattttcgc
       61 cggattttat tcggattatc ctgcttatcc tcgtgctgtt tctcacgtag tctataattt
      121 cctttttaag cccacaggag agcaacaatg acaatccata agaaaggtca ggcacactgg
      181 gaaggcgata tcaaacgcgg gaagggaaca gtatctactg agagcggcgt gctgaaccaa
      241 cagccgtatg gatttaacac gcgttttgaa ggcgaaaaag gaaccaaccc tgaagaactg
      301 attggcgcag cacatgccgc atgtttctca atggcgcttt cattaatgct gggggaagcg
      361 ggattcacgc caacatcgat tgataccacc gccgatgtct cgctggataa agtggatgcc
      421 ggttttgcga ttactaaaat cgcactgaag agcgaagttg cggtgccggg tattgatgcc
      481 tccacctttg acggcattat ccagaaagca aaagcaggat gcccggtttc tcaggtactg
      541 aaagcggaaa ttacgctgga ttaccagttg aaatcgtaag cgttgccgga tgacgcgtca
      601 ggcgcgtgaa tgcctgatgc gtcagcacaa tcacatcagg ctgagaaggg attgttctga
      661 catggctatg cgttgtcgtt gtccaactaa acgtagcgga aatcacagta tttaagtgac
      721 agtgtcacgt taaataaaaa cccgcgagtg cgggcgagag gaatttgtca gattttcagc
      781 ggtaacacgc tgctcatcca gcctggaatt tcttcggcgg gcagggggcg ggaaaagaaa
      841 tatccctgaa taacgcgaca gtggatcttg cgtagcatct caaattgctc tttggtttcg
      901 atgccttcag ccacgacggt taaattgagg ctttgcccaa tgctggtaat ggcttcgagt
      961 aaggcaagga tgcgtttttc ggtcagacaa cgatcgacaa aacttttatc aattttgatt
     1021 tccgttaccg gaagactgac taagcgggat aatccggaaa agcctgtacc aaaatcatct
     1081 accgataaac cgatgcccat atcgcgcagg atctgaatgc gcttaaagat ttcggtatcg
     1141 tgttccatca tcatgctttc cgtgatttcc accgtcagct ggtggccgtc aataccccag
     1201 gcgtgcattg catcagatac ctgattaggc agctgattac tgcgaaagtg cagcgcagac
     1261 aagttaacgg ataacgccgg gatatgaata ttctggctac gccattctgc taactgacgg
     1321 caagcttccg cgatgaccca gcgcccaata ttttcgattt caccaatctc ttctgcgaga
     1381 ggaataaacc gtgaaggggg cacatgacca tgcaggggat cgtaccagcg agcaagggct
     1441 tcgatgccgt acagttcacc cgtttctgcg aagatttgcg gctgataaac cagtttaagt
     1501 tggttattgc taatcgcttc ttttagcgct gcgcctaaaa ccaaacgctc ttttaccatt
     1561 tcgttcatcg ccgggctgaa gaactgccag ccgttaccgc catttttgcg aataaaatcc
     1621 attgcattgt gagcagtgga gagcaagtaa tcgcggtttt taccctcgtc gtagctgatg
     1681 ccaatgctca aggtgagcgg gaatggttta tcgtcaatca ttatcggctt gctgaccaca
     1741 ttccgtagct catcggcgat ctgggtaatg ttactgacat cgttttccag gctcacgagg
     1801 acaaaagacg caccttcaat acgacagaga tactgatccg gtttgagttt ttcacgaaag
     1861 cgattgacca cttccagcaa tgcctgatcg gcccacgcat agccaaggct atcaatcaca
     1921 tcctgaatat ggtcaacacc gatgagatac accaccggag agacggcttt gtcgaccagg
     1981 tcatcgaggt aattgtgcag gttattgcga tttggcagac cggtcatcgg atcaaattgg
     2041 atgagttgtt caatatgctg acggcttttt tcctgttcca gcgccagcgc ggccatatgc
     2101 tggctgatat ctgccacgcg ttcgataaag gcgctggttt ctgctcctga cgaggtttta
     2161 atttgcagga tccccgcagg cgcgccatcg cgctgacgaa tggtcgctga ccagctttgc
     2221 gcattttgaa cttctgcacc gtgcgaagat gacgcccagt gtatcggcat cccgttgcgc
     2281 agcgcgaaca gcgaaacatg cgattcgttg agaacagatt cgatgttacg acaaatgatt
     2341 tcccccattt catgaaatgg ggggctgctg cacatggcgg cgagaatatt gccttcaagc
     2401 tggcggattt gcctttcttc ggtgatatcc gagaatgtca ttaccaggtt ctgcagatgc
     2461 gcgagcacgt cataaaccgg gctgatagag gctttaatcc agattttttc accggtgcgc
     2521 gtcaacagca gaaattcgtc ctgatcgcgg gcggttttcc atagcaactg ttgtaaacga
     2581 atacggttat cagcagggaa ttctggaatg ttcaggagtg tatcgggctg cataccgctg
     2641 gcttcgttaa tgcagtaacc aaacatttcg gtaaatgcgc gattgcactg cacaatatgg
     2701 cgttccggat cgaggacaat caccggtcgg tcgagatggt caacggcaat aatcaattgt
     2761 cgggtctgtt ctttttgcgc catttctacg ctggcatccc gtaccagcgc caggtaataa
     2821 actttcccct cggcgctcac tttcgatagc gcaaaacggg tccagatttt actgccgtct
     2881 tttttctcca gctgcagctc ccgactcatc ccctcgacgc gcgctttacc accttcacgg
     2941 ttgtgacgaa tgtattgagg atgcgcagga cgcaaatccc gcggaatcag catatcaatg
     3001 ttattgccaa tgacttcttc acgtttgtac ccccagagct tctctgcggc ggggttgaaa
     3061 aacatcactt catcattttc gttaattaac accgcgccca tcatattttg ctcaagggcg
     3121 gggaaaaaaa tgccatcggc ggcattatcc gcatcggtta gcttcatgat tacctctgca
     3181 tcctggcgca tctaaagact ggctttacag agttcaacac ggtttctacc tcgtcttttg
     3241 gcgatataca gagcttcatc ggctatttga atgaggcgct catagtcagg atgaccatta
     3301 aacatggcgg caccgatgga aagtgagagg gcaatatcct cgccgtttgc ggctttaaaa
     3361 taatgtcgac ctttatggtt aaaccgcagg ccaaaatcaa ccaaacacaa cgtgcgggat
     3421 cgactaactg caccatattc tcctgaaata tgaagatata ctgaaaagaa ataagcgact
     3481 taggacagtt tcaatctacg ctactgttct tcagaagagt atagcccatc gtaattattt
     3541 ttcggtgaca gcgaatatcg tatgtttttt catattcata catttttatt agggggatta
     3601 tggctgttta actaagtgtg gttaattgcg tggttaattt gacgtattta agcatgattg
     3661 ctagtgggat agttcaagag ggtaacaagc cagtgggtaa agcaccggct tgttacaaag
     3721 taagaatggg agtttaactg ccccaacgac tttgcagata gctgaccgct tgttgcgtct
     3781 gcggtttatt cagatagtcc tcacggaaca agattgtgcc gctaatttcc ggcacagcat
     3841 cgttaagatc gagctgcttt ttcagttccg gtacgccgcc gttaatcatc cagtctggct
     3901 ctatctttga aggttcaccc actttataga aggcgatacc gatatacagg cgggtcctgg
     3961 tcggtttaac gacatccgcc caccattttg ccaacacgtc ataacgcgcg gcactccgtg
     4021 agaatggcca gtaaatttgg ggagcaatgt aatccagcaa cccttgttcc acccatcgac
     4081 gggtatcagc gtaggattcg tcataggctg ccgcgcctcg ggtatcggaa ccgagcggat
     4141 cgtgtgatcg gttacgccac acgcctgccg ggctaacgcc aaattcgact tccggcttaa
     4201 tgcttttaat ggtgtgcgat accttcgcaa ttaactgctg agtattgttg cgccgccagt
     4261 ctgcttttga cgcaaatgcg ccgccgtatt tgcggtacgt ttcgttatca tttagccgtg
     4321 aacctggtga ctcagtatag aaatagtcat caaactgcac gccatctact ggatagcggg
     4381 aaaccacttc cgcgactatt gatgtgatcc agtcctgaac ctcagggatg cccgggtcga
     4441 ggacaaagcg atcgccggac gttctgatcc agtcgcggtg ttgcacatag acgctcgccg
     4501 gttgttgaga cagagtgcta ttcagttccc tgatagtacc gggcttcgta ttaaccgata
     4561 cgcgataggg gttaaaccag gcgtgtactt tcatcccacg cttgtgggct tcgtcgagca
     4621 taaattgcag cggatcgtaa cccggatttt caccaatctt accggtcata agatcggacc
     4681 acggcaaaat tttcgatggc cacagggcag taccatccgg cttgacctgg aaaaagaccg
     4741 tgtttatgcc gagacgttgc agatgatcca gtttgtcgat catcgcctgt tgttgtacac
     4801 gtgcccggct ggtggggtta ctaatgttaa ccgaggaaac cggtggccag tcgaggcgag
     4861 acaccgtggc cagccagatg ccacgcatcg gttgtgacga ttgttgcgtc gtggctggtg
     4921 gctttgagcc cgctggtggt gtcaccatgg actctggagg cgtgctttta caactacaca
     4981 gtaaaagtgc cagtgcaacc agtatcgctg gtcttctaat cgctaatttc tcgtttcggg
     5041 agcagatatc catatgtgct ggtttccggt aaacagatgt gcgctcattc tcgtacttat
     5101 cccggttaag tcaatacgac aacaagcacg aaaaagggag cgatgaatta tcgctccctt
     5161 gtcttataac cattcagaca tggttagtgt ttcttgtcat tcatcacaat atagtgtggt
     5221 gaacgtgcac gcgggtgcag gaagaagtga ccttttggtg cgttctgact gttgattggc
     5281 tccagagtga cgccggtatt cgctttgctt ttacgatcat gaacagcata gagaataaag
     5341 ggcagggcaa gtaccaccag gaaactaacg accagtaatt caacatacat atcggtagag
     5401 tcaccctgga tgttatccgg cggcaggaag gaaacaataa acgccataat tgaagtcagc
     5461 agaccgacaa tagccacgac cagtttcacc cctttaccac cagggatatt aaatgtgcgt
     5521 tttaagtcag gatgtttaag aaccaacaca atgtagccaa taaacagcat gaaataagca
     5581 cacagataaa tcaccaccgt cagcgccagt gcgatcagga aggacatgtt gttaccgcca
     5641 ccggtattgg tgaggatgat caacgcgata gaagtaatca ccaactgcga aatgaccagc
     5701 gttaccggta cgccattttt gttcatttta gcgaatgccg ctggcagcag gtttttctgc
     5761 gctgtgacat acatcccgcg agaaggacca acaatccagg aagcgatttc cgccagaaca
     5821 cccagcaaca gcagtgcgga gatcacgcga accgtccact caatttccgg tgccacatgt
     5881 gacatcagaa cggtaaaggt ttgcattacc cctgcggaga ggttgatttc attacccgga
     5941 atgaccatcg caatagacaa accgccaaca gagcttaagc agattgccgc caccatcagc
     6001 agtaacatag ccagtggata gtcgcgcccg gggttgctca tttcattgac gtgggttgcg
     6061 gaagcttcaa cgcccatata actcaaaatg aaggcaacaa atacaacgag ggtgcccact
     6121 ttagagaagt cagggaagaa ggtcttcgca tccatttcga tagcaacggg ggcaccggag
     6181 tgcagataaa tagccgctaa tgcgatcaaa ataaatgcag gtaacaggat accggcgaag
     6241 aagccaactt tagcaattcg agccgtgtat ttcgtgccac caaactgcgt taatgccagc
     6301 gcccaaagaa tgatgagtgc tgcaatagtt ttggtaatgg ggtcttcatt cagcgctggc
     6361 catttcagga tgtaggagag tgctcctaac acgaaataga gcatcggaat aaaaccaatg
     6421 gcgatttgca gatagccaaa tgagatcgct gcaaatcccc atctcggccc cagagtattt
     6481 gatacccagg cgaagacgcc accttcttcc cagccgtcga cggtagccat ttccgcagca
     6541 caaagtccca cgggaataaa ccataaaatc ccgcctaata gcaggaagaa gactaatgaa
     6601 aagcccgatg ttgcgaaggt agggtattca taaacagcca ttaccatcga tgccgttatg
     6661 gcaaaaaatc cgagtaatgt gagctgctta gctttacctg tctgtactga tgtagccata
     6721 ttatccccct aaaacggtat tcctgtcgga accgcacctg ttttgttctg aagcgtattc
     6781 agaacaatat tttccgttgc taatgccagt gaacagactt tggaaattgt cccgaaacgg
     6841 cttcgtttcg ggacaccgtt accgttaaac gttatcaggt gtgtttaaag ctgttctgct
     6901 gggcaatacc ctgcagtttc gggtggtcgc tgagatattt cagggaggct ttgtagtctt
     6961 ccagcaacag ttcagcaaag tccatttcga agccgcgacg acacataatg cgcatcacca
     7021 cgatgtcagt ggcttcaccg ccgagagtga aggccggaac ctgccagccg cgcagacgca
     7081 gacgttcaga gaggtcgtac agggtgtatc ccggatcttc accatctttg agtttgaagc
     7141 aaaccgccgg gatgccttcg tccgggcgac c
//
LOCUS       DADSZD010000194         7109 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.196, whole genome shotgun sequence.
ACCESSION   DADSZD010000194 DADSZD010000000
VERSION     DADSZD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.196"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(2..94)
                     /locus_tag="J1Z28_003915"
     tRNA            complement(2..94)
                     /locus_tag="J1Z28_003915"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="J1Z28_003916"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="J1Z28_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBA7718697.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="J1Z28_003917"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="J1Z28_003917"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBA7718698.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFDAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKSVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3589..4089)
                     /gene="recX"
                     /locus_tag="J1Z28_003918"
     CDS             complement(3589..4089)
                     /gene="recX"
                     /locus_tag="J1Z28_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBA7718699.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4158..5219)
                     /gene="recA"
                     /locus_tag="J1Z28_003919"
     CDS             complement(4158..5219)
                     /gene="recA"
                     /locus_tag="J1Z28_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBA7718700.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5299..5796)
                     /gene="pncC"
                     /locus_tag="J1Z28_003920"
     CDS             complement(5299..5796)
                     /gene="pncC"
                     /locus_tag="J1Z28_003920"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBA7718701.1"
                     /translation="MTDSELMQLSEQVGQALKVRGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(5941..7026)
                     /gene="mltB"
                     /locus_tag="J1Z28_003921"
     CDS             complement(5941..7026)
                     /gene="mltB"
                     /locus_tag="J1Z28_003921"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBA7718702.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
BASE COUNT         1655 a         2007 c         1720 g         1727 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatcg tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acactcaatt agtctgtaaa
      241 aacgtcaaaa agagtgtttt atcaacagaa aaacggaggt ctgacagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacaa tggagtgaaa
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatctaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc acccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacatct tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcactc tcctgtgcgg
     1201 cagcctgttc tttaagctgt tgtaactctt tttccagctg acgcgtacgt tccagtactg
     1261 aacgcacttt atcagccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggcga tagcgccttc tccggtcacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacgcgctc atcatacttc tcgccaaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagatccat gatgttggtt tcgatcggca agttacggcg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tctggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcactttgtc gttaaccagt gaacctttct gcgatacatg agtacccaaa acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaacatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggcctgcat tgatggcatc aaccgattta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcgc
     2101 tgtcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcacgac
     2161 gacgctgctc ttccattgct gcgtcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccgtaggt gtcgtacagg cggaaagcag
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc agcgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacctgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattacc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaatgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggtttcgg cagcggttcc atcgtgccat cggcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccgcccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatccct acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgctaccagc cgttgcgtgt cgttaaccgc ccggctggta actgaaaagt
     3541 gggaataaga taagttttct tgactgggaa gtaaaatccc gtatgcgttc agtcggcaaa
     3601 atttcgccaa atatcctgga tatcttccat cagatagcca cgatagagca gaaaacgctg
     3661 aatcttaact ttttctgaaa agacagttgg caaaggttcg ccatattttc gcgtcgcctg
     3721 atcgcgcgcc agtgcgcacc agtcgatgtc acattcacgc atcgcttttt ctgtcgcttc
     3781 gcgggaaata cctttctgat tcagttcctg acgaatacgc gcaggtccat aacctttgcg
     3841 gctacggctg gcgataaagc gcgcaacaaa tcggctgtca tcaagatagc catgttcgtg
     3901 gcaccaggca ataacgcgct cgtaatcttc tgccgtggca tcaatctctt ccgggccatt
     3961 tttgcccata atcggtgccg cgagtttacg tcgcagttct tgctcactgt gatcgcgcac
     4021 cgccagaatg cgtaccgcac gatccaacag gcgagcatat gccgggcgac gggatgttga
     4081 ttctgtcatg gcatatcctt acaacttaaa aaagcaaaag ggccgcagat gcgacccttg
     4141 tgtatcaaac aagacgatta aaaatcttcg ttagtttctg ctacgccttc gctgtcatct
     4201 acagagaaat ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg
     4261 atctctttcg cggtttccgg gttatctttc agccaggcag tcgcattcgc tttaccctga
     4321 ccgatcttct cacctttgta gctgtaccac gcgcctgctt tctcgataag cttctctttc
     4381 acgcccaggt caaccagctc gccgtagaag ttgatacctt cgccgtagag gatctggaat
     4441 tcagcctgtt taaacggcgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta
     4501 cccaccacgt tttcgccctc tttcaccgcg ccgatacgac ggatatcgag acgaacagag
     4561 gcgtagaatt tcagcgcgtt accaccagta gtggtttccg ggttaccgaa catcacacca
     4621 attttcatac ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc
     4681 gccagcttac gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg
     4741 atttcgcctt cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg
     4801 tctactgcgc cagaacgtgc cagggcatca cagatttcca gtgcctgctc gccggtatcc
     4861 ggctgggagc acagcaggtt gtcaatatcg acgcccagtt tacgtgcgta gattgggtcc
     4921 agcgcgtgtt cagcatcgat aaacgcacag gttttacctt cgcgctgcgc tgcggcgatc
     4981 acctgcaacg tcagcgtggt tttaccggaa gattctggtc cgtagatttc gacgatacgg
     5041 cccatcggca gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg
     5101 gtttccacat ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt
     5161 ttctcaatct ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt
     5221 tttactcctg tcatgccggg taataccgga tagtcaatat gttctgttga agcaattata
     5281 ctgtatgctc atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc
     5341 atacgcagta gcctgacgac gcaccgcatc acggtcaccg ctgaagcatt cccgccgggt
     5401 aatgccttca ccacgggcag tggcaaaagc aaaccagacg gtgccgacag gtttctcttc
     5461 actgccgcca tccggcccgg cgataccact aatagacacg gcgtaatcag cacgagccgc
     5521 tttcagtgcg cctatcgcca tttccaccac aacaggttcg ctcaccgcgc catgctgcgc
     5581 cagcgtctct tcgcgtacac cgatcatttg cgcttttgct tcgttactgt aggtgacaaa
     5641 tccgcgttca aaccaggcgg aactgccggc aatatcggta atcactttcg ctacccaacc
     5701 gccggtacaa gactcagcag ttgttacggt tgcgccacgg actttcagcg cctgcccaac
     5761 ctgttcgctt aactgcatca gttcactgtc agtcatcaca acctctgtca gtcataaatt
     5821 tatgccggac aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag
     5881 gaggattcag aaaaaagctg attagccaga gggaagctca cgcccccctc tggcaaatag
     5941 ttactgcacg cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg
     6001 ggtgctgtgg ttataacggg taatggtgta gaagttcggc agaccgtacc agtactggta
     6061 gccggtgcca acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg
     6121 tggcgttaaa cctgccgcgg caagctgcga aatgctgtac ctggttttga agccatttgg
     6181 caggcctggc gcctgaccgt ttgccattac cgcgacctga tcgcctttca cccagccgtg
     6241 cgctttgaaa taattcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg
     6301 cccgtcgccg ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtccgta
     6361 gcccatcgcc ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc
     6421 catcagcagg aaggtttcca gctcgccaga aaaatactcc gcgcggcgtg ggtagttaaa
     6481 tgacagcgtt gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt
     6541 ttcaacgccg ataatcccga cgataatttc cggcggtaca ccatacacct gccacgcgcg
     6601 attcaacgca tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt
     6661 aataaatttt ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga
     6721 tgtggttggt gcctggttat ccatcagccg cagtaccgaa tccagacgtt tcgcctggga
     6781 gagaatttcc tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa
     6841 ctgctgggca ttcgggttat tggcgaagtc accgcccatc tgcatcacat tgtgctgcgg
     6901 ctcaagcagg aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg
     6961 cttgctgcta caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt
     7021 gaacatgagg ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcccgcc
     7081 ccgcacaagg aagcggtagt cactgcccg
//
LOCUS       DADSZD010000195         7100 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.197, whole genome shotgun sequence.
ACCESSION   DADSZD010000195 DADSZD010000000
VERSION     DADSZD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.197"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            55..368
                     /locus_tag="J1Z28_003922"
                     /pseudo
     CDS             55..368
                     /locus_tag="J1Z28_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007794049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(531..1205)
                     /locus_tag="J1Z28_003923"
     CDS             complement(531..1205)
                     /locus_tag="J1Z28_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBA7718703.1"
                     /translation="MMHVRNFSYYTPAEPDVAGAIYLKSEDGQDWYECQSRFAEDTLK
                     VVYDSRGVITGYGKDIKLLWPVNQSVAEVPDTPESLKIDLSGRWGFDGEKITDLLTAD
                     RAREQKGAEINAWRSAMENAEYVFEYNGRKWDYGKVTQVRLEPSVAAAKAGILPEGFF
                     WTDAENNDVPMTAEALIALSAAAEKAMFQKGMEIHIRQRTMKKELEKLTSADEILAYP
                     VGWEMK"
     gene            complement(1202..1426)
                     /locus_tag="J1Z28_003924"
     CDS             complement(1202..1426)
                     /locus_tag="J1Z28_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718704.1"
                     /translation="MESYVFSPSANMFYPVSLRAVYEAAGNWPVDGIVVDYAVYKVFA
                     ADAAPAGMKRGVGTEKMPVWVPVSEEGTGK"
     gene            complement(1436..2896)
                     /locus_tag="J1Z28_003925"
     CDS             complement(1436..2896)
                     /locus_tag="J1Z28_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7718705.1"
                     /translation="MAVRISGVLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEAGLYRVTLNTEGRMPSYVGDILVKADSAPGTLNKFLMDFEDAPYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDPGPQGVQGPKGETGATG
                     PKGERGETGAQGAPGPKGDTGATGPEGKAGPVGPKGDRGDTGLRGERGEKGEKGDPGG
                     PPGPKGDTGPAGPQGPKGDTGPQGPKGDTGPQGPQGKSGSLEDITDREAFIRELGVPR
                     AYGRDISTGAGEWTTEEFVAWLKEQGAFDHTFWMMKVALPAAMNKVITDTGLGKINLG
                     GCAVEVAGTYNAAIVRVTIGEYAATGFLNGTVCTCTVYGDTRKYHWRVDYSTRNKPTT
                     ASLAVNGWERDEATGRIRQWGRIDVSQADGKALSLHSVRFPVAFPSAVLNVQVSAVGD
                     QHTIQMYTLSEPSLQTVTLRTIKGSYGTFFWEATGY"
     gene            complement(3038..3178)
                     /locus_tag="J1Z28_003926"
     CDS             complement(3038..3178)
                     /locus_tag="J1Z28_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBA7718706.1"
                     /translation="MSQKPLKTTVICITVVLIIWTTHSSLCEFRFRIAGAEIAAFLQC
                     KQ"
     gene            complement(3376..6852)
                     /locus_tag="J1Z28_003927"
     CDS             complement(3376..6852)
                     /locus_tag="J1Z28_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7718707.1"
                     /translation="MGKGGGKGHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDMDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERSGHWVTEKDITINGKTT
                     SQYLTSVIIGNLPERPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQSYPNTAIV
                     GLQVDAEQFGGQQMAVNYHIRGRIIQVPSNYDPGKRTYSGIWDGTLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYALGQYCDQPVPDGFGGTEPRMTFNAYLSQQRK
                     AWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWTYTAGNVVTGEDGVSFHYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLW
                     VIKTELLETQTVDFSVGAEGLRHTPGDIIEISDNAYAGTVIGGRILSVDNASRTLTLD
                     REVTLPEKGTSTVNLINGSGKPVSVDVTGQPSPDRIQISTLPDGVVEHSIWGLALPGL
                     RRRLFRCVAIRENADGTFAITAVQHVPEKEAIVDNGATFEPLSGSLNSVTPPAVQHLT
                     VEVSASDGQYLAVAKWDTPRVVKGVRFSLRLTSGTGENSRLVTSAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAAPASIELTPGYFEIMVVPKLAVYDPTV
                     QFEFWFSEQQITDLLQVEKSARYLGTGSQWSVTGSRIKPGQDFWFYVRSVNLVGKSAF
                     VEASGHASNDAAGYLEMFREKIGKLHLAQELWSQIDNGQLKDEMAEMKTTITETRNEI
                     TQTVNKKLEDQSATIQQIQRVQKETDDSLSALYMLKVQKTKNGIPYVAGIGAGIEDVD
                     GQPLSNILLQADRIAMINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNINANSGTLNNVTINQNCRILGKLSANQIEGDLVKTVGKA
                     FPRNNSYASGTITVTVYDDQGFDRQIIIPPVLFRGARHEHQSSKDHTSTWYSTCQLQV
                     QMNGRVIFHEPATDVTRVFSSVIDMPAGRGHVTLTFNVWSNGVNHWTPTTYISDLLVV
                     VMKKSTAGISIS"
BASE COUNT         1455 a         2238 c         1618 g         1789 t
ORIGIN      
        1 ttttttcagc ccccttttat ctaatcgctg caaaaaacaa caaatagtat aaaaatgagc
       61 aatcctaatc catacatgac gtgtggtgcc tgttgtgcat tttttcgtgt ctctttttac
      121 tgggctgaag ctgacgattc tggcggaaac gtcccggcca gtctcactga gcaaacatcc
      181 ccctttcatc gctgtatgcg cggcaccaat cagaaaaatc cccgatgtgt cgctctggcc
      241 ggcaccccgg tgaaaacgca tattgctctg tttacaaaaa ccgtccgtcc acatgcaggg
      301 aattcgcaat gtccggcgaa aatggcgaaa tcaatgaggc atgtaaccgc gcacgagcaa
      361 tatattgact tccgaaaatc tgaaaaatac tgatgcccgc gaaaatacga gcagcatcac
      421 agttacagca gaatttcctg tggcagtatc tggttgcatt cgtaactatc tacactaagt
      481 catcattatg accttctttt atgggggatg gtttctcccc ccttttttta ttatttcatc
      541 tcccagccaa ccggatatgc cagtatttca tccgcactag tcagcttttc cagctctttc
      601 ttcatcgtgc gctggcgaat gtggatttcc atccctttct gaaacatcgc cttttctgcc
      661 gcagcactga gcgcgatcaa agcctccgct gtcatcggca cgtcattatt ttctgcatct
      721 gtccagaaaa atccctccgg cagtattccc gcttttgctg ctgccaccga cggctcaaga
      781 cgaacctgag tcaccttgcc gtagtcccac ttccgtccat tgtactcaaa cacatactcc
      841 gcattttcca tggcacttcg ccaggcattt atttctgccc ccttctgttc acgggctctg
      901 tcagccgtca gcaggtcggt gattttttcc ccgtcaaacc cccagcgtcc gctcaggtca
      961 attttcaggc tctccggtgt gtccggcact tcagccacac tctggttcac cggccacaac
     1021 aacttaatgt ccttaccata tcccgtaatc acaccacggc tgtcatacac caccttcagc
     1081 gtgtcttccg caaacctgga ctggcattca taccagtcct gaccatcctc agatttcagg
     1141 tatatcgcac ccgccacatc cggctctgcc ggtgtgtaat acgaaaaatt tctgacatgc
     1201 atcattttcc cgtgccctct tccgataccg gcacccagac cggcattttt tccgtaccga
     1261 cgccccgctt catgcccgcc ggtgcagcat ccgctgcaaa aactttatac acggcatagt
     1321 ccaccacgat accatccaca ggccagttcc cggcggcctc ataaacggca cgcagggata
     1381 ccggataaaa catattcgcg gacggtgaaa aaacataact ctccataatc cctcctcagt
     1441 atccggtcgc ttcccagaaa aatgtcccgt aactcccctt tatagtccga agtgtcaccg
     1501 tctgtaatga cggttctgac agggtataca tctgaatggt atgctggtct cccactgcag
     1561 atacctgaac attcagtaca gcactcggaa aagcaacagg gaaacgaacg gagtgcaggg
     1621 acagtgcttt accatcagcc tgactcacgt caatacgccc ccattgccgt atccggcctg
     1681 tggcctcatc acgttcccag ccattgacag ccagactcgc cgtcgtgggt ttattcctgg
     1741 tactgtaatc cacccgccag tggtatttcc gggtatcgcc ataaacagta caggtgcaga
     1801 ccgttccgtt gagaaacccc gttgcggcgt attccccgat ggtcacacgg acaatcgcag
     1861 cgttatatgt tcctgccacc tctacagcac atccgcccag gttaattttc ccgagtcccg
     1921 tatctgtaat aaccttattc atcgccgcag gcagtgccac cttcatcatc cagaaggtgt
     1981 ggtcaaatgc cccctgctct ttcagccagg caacaaactc ttctgtcgtc cactcacctg
     2041 cgccggttga aatatcacgc ccgtatgctc tgggcacacc aagctcacgg atgaaggctt
     2101 ccctgtctgt aatatcttca aggctgcctg actttccctg aggcccctgc ggtccggtat
     2161 ccccctttgg tccctgtgga ccggtatctc cttttggtcc ctgcggcccc gccgggcctg
     2221 tatcgccctt tggcccggga gggccacccg ggtctccttt ctctcccttc tcgccccgct
     2281 cacctctgag ccccgtatcc ccccggtcac cctttggtcc cacaggaccg gctttaccct
     2341 cgggtcctgt ggcaccggta tccccttttg gccccggagc accctgtgca cctgtttcac
     2401 cacgctcccc ctttggcccg gttgcacctg tctcaccttt cggtccctgt accccctgcg
     2461 gtcccgggtc gcctttatca cccttcggcc ctctggcatt ctctgcccgt tttttggcct
     2521 cctctgcact ggctgcagag gcttcagcac gcttcagtat ttccgctgcc accgcttcca
     2581 gctcagcgag ggctttcgga taatatggcg catcctcaaa gtccatcagg aatttattca
     2641 gtgttcccgg tgcagagtcc gccttcacca gaatgtcacc gacgtatgac ggcatacgcc
     2701 cttctgtgtt cagggtcaca cggtacagcc ccgcttcaac atccatactg tagctgcctg
     2761 tctcccccgg ctgcccgtat gccaccgtgg tgacaatcac cgtttcggtg gtacgtcgcg
     2821 ctttcagctc tatcgtgcat cccggtaccg gcttccctgc gccgtccttc agcacaccag
     2881 atattctgac tgccatagat tttccccata aaaaaaccgc agtgccggtt tcccggcctg
     2941 cggtaaaaat cgtggtttac aggattaaaa aggtgccatc cggctgacca ccttcagcaa
     3001 ccggtcagcg ggggacaggg tcccccgcca cggtttctta ctgtttacac tgtaagaacg
     3061 ccgcaatctc cgcgcccgct atccggaacc ggaattcaca cagtgaactg tgggtggtcc
     3121 agataatgag cactaccgtg atacaaatca cggtggtttt taacggtttt tgcgacataa
     3181 cgcttgctcc ttttacggag aggcgctaac ctttcacttg cttaggatga atggtcaggc
     3241 ctcgggttaa acatgaatgt tttgtccggg gccttttcac atccggcctt caggtgttcc
     3301 ctccggccat cagccaaagg cacccgcgca tactgtacgg tttttatctc cttccggcaa
     3361 ttccggtcat catgttcacg aaatactgat acccgccgtg gattttttca tgaccacaac
     3421 cagtaaatca ctgatgtatg ttgtcggcgt ccagtggttc accccgttcg accacacatt
     3481 aaacgtcagg gtcacatgac cccgtcctgc cggcatatca atgacggacg aaaacacccg
     3541 ggtgacatcc gttgccggct catgaaaaat cacccttccg ttcatctgca cctgtaactg
     3601 acacgtggaa taccatgttg aagtgtgatc tttgctggac tggtgttcat gtctcgcccc
     3661 ccggaacagt accggcggaa tgatgatctg cctgtcgaat ccctggtcat cataaacggt
     3721 gaccgttatc gtgccactgg cataactgtt attccgtggg aaagctttcc ccactgtttt
     3781 gaccaggtca ccttcaatct ggtttgcaga cagttttccc agaatccggc agttctggtt
     3841 aatggtcaca ttattgagcg tgccagagtt cgcattaata tttccgctga tatccgcatt
     3901 acgggccgtc agcctgcctt ccggcgtcag ggagaacgtc gggggattgc cggacgaggt
     3961 gatgctcacc gcaaacagtc gcttcaggaa cacgtcgttc atgaacaact gattcccctg
     4021 cgccacaaac agcggcgtgg tgttgccgtt ctccgggtta atcatcgcga tacggtccgc
     4081 ctgcagcaga atattgctca gtggctggcc atcaacatcc tcaatccctg caccaatacc
     4141 ggcaacatac ggaataccgt ttttcgtttt ctgcaccttc agcatgtaca gtgcgctcag
     4201 gctgtcatcc gtctccttct gcacacgctg tatctgctgt atggtggcac tctggtcttc
     4261 cagcttttta ttgaccgtct gtgtgatttc gttccgggtt tccgtgatgg tggtcttcat
     4321 ttccgccatc tcatccttca actggccgtt gtcaatctgc gaccacaatt cctgtgccag
     4381 atgcagtttt cctatctttt cccggaacat ctccagatat ccggcggcat cattactggc
     4441 atgcccgcta gcttccacaa acgcggattt accgaccagg ttaacgctgc gtacgtaaaa
     4501 ccagaaatcc tgtcccggtt taatacggga accggtaaca ctccactgac tgcctgtccc
     4561 cagataacgg gcagatttct ccacctgtaa cagatctgta atctgctgtt ccgaaaacca
     4621 gaactcaaac tgaacggtcg gatcatatac agccagtttc ggtaccacca ttatttcaaa
     4681 ataacccggc gtcagttcaa tgctggcagg tgctgccggt gcgttaatcc ggaacgtggt
     4741 ggtggcaggc tccccctgct ggccatagct gtttatcgcc ctgaccgtca gggtgtactc
     4801 ccccagaggc agaccgctga aacggtgctc cgtgtcggcg gtgatggcgc tggtcaccag
     4861 acggctgttt tcaccggttc cgctggtcag gcgcagactg aagcgcacgc ccttcaccac
     4921 cctcggcgtg tcccattttg ccaccgccag atactgaccg tcagacgcgc ttacctccac
     4981 cgtgagatgc tgtactgccg gaggtgtgac actgttcagc gaaccggaca acggctcaaa
     5041 ggtggcacca ttatccacga tggcctcctt ctccggcaca tgctgcaccg ccgtgatggc
     5101 gaaggtaccg tccgcatttt cgcggatcgc cacacaccgg aacagacggc ggcgcagtcc
     5161 cggcaatgcc agcccccaga tactgtgttc caccaccccg tcaggcagtg tgctgatctg
     5221 tatcctgtca ggtgatggct gcccggtaac atccacactc accggcttac cactgccgtt
     5281 tatcagattc accgtggatg tgcccttttc cgggagggtg acttcacggt caagcgtcag
     5341 ggtccggctg gcgttgtcga cggacaggat ccgcccgccg ataacggtac cggcatacgc
     5401 attatcactg atttcaatga tatcgccggg cgtgtgacgc agtccctccg cccccacact
     5461 gaaatccact gtctgtgttt ccagcagttc ggtctttatc acccacaggc ctgcccggtg
     5521 agcctgacca cggctggtac agccgaacgc atccatcttc agaagattgc gtccgtaacg
     5581 cagtatcgca tccggatcct ccaccagttc agtcgatgtc tgccagccat tctgcggatc
     5641 ggtgtaattc acctcaactg ccgtgtgtct gtccttgagg gcactgaagc tgtaatgaaa
     5701 acttacccca tcctcccccg tcaccacatt accggcggta taagtccaga caacatccga
     5761 tggtctgtcc tgaacaaacg tcagcgtctg tccgttccat accggcatac agcgcattgc
     5821 agagcagaaa tcccccagca cgtcccacgc cttacgctgt tgtgacaggt acgcattaaa
     5881 ggtcatacgg ggttctgtac caccaaagcc atccggaacc ggctggtcac agtactgtcc
     5941 gagggcatag agcgcccact tgtccacatc cgcggccccg aggcgctttc ccataccata
     6001 acgtggatgc gtcagcatat cccacagaca ccatgccggg ttgttgctgt atgccggttt
     6061 cagagtcccg tcccagatac cgctgtatgt acgttttccc gggtcatagt ttgacggcac
     6121 ctggatgatg cgcccgcgga tatggtagtt caccgccatc tgctgcccac cgaactgctc
     6181 cgcatccacc tgcagcccca ctatggctgt gttcgggtag ctctgtttca catcgatgat
     6241 ttcggtgtat gacgaccaga gcgttctgtt ctgcagctgg tctgtggtac tgtccgccgt
     6301 ctctctgacc atccggatat taaagggacg ctcagggagg ttaccgatta tcaccgacgt
     6361 caggtactgc gaggtggtct taccgttaat ggtgatatcc ttctctgtca cccagtgccc
     6421 gctacgctca agctgaatca gcaaccggac agaagaggga ttacggtcac cctttgaggt
     6481 cgtctccacc agtgactgaa ccccgaaggt gacccgcaga cggtcaatgt tcgctgacgt
     6541 gatggtgcgc gtcaccggct tcgccctcgt cacctccacg cccagtgcgg tttccgcacc
     6601 ggaagactca aagccttccg gcggcgtctg ctcctgctcc ccggcacgcc agacggctgt
     6661 cacgccatgt atcacgggat taccgtccat atccgtcagc ggggttttgt tcaccagaat
     6721 actctgcagt cccttcaccg gaccttcaac cggcccttca ccaatggcat caatcacgct
     6781 catcatctgc gtggacttca ggttatcctt tgcctcacgc ggtgtgtgcc ccttgccgcc
     6841 ccccttaccc attgcgtcac cctttagtgt ctgatgtcag tctctgttta tgtacaaaaa
     6901 caacaggcac cccggagggt gcctgtctgc tgagtgaata aaatttctga atttcttcac
     6961 acttccggca attacctgta gccaccataa ttacgctgcg ttactgttct tctgcaaaaa
     7021 ataactcctt aaagttcatc ttcatcgttt tccccacagt ctggccaata ctgtgggtat
     7081 tttttatgtt tattctcacc
//
LOCUS       DADSZD010000196         7086 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.198, whole genome shotgun sequence.
ACCESSION   DADSZD010000196 DADSZD010000000
VERSION     DADSZD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.198"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            241..1116
                     /gene="rluB"
                     /locus_tag="J1Z28_003928"
     CDS             241..1116
                     /gene="rluB"
                     /locus_tag="J1Z28_003928"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBA7718708.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEI
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1156..1746)
                     /gene="cobO"
                     /locus_tag="J1Z28_003929"
     CDS             complement(1156..1746)
                     /gene="cobO"
                     /locus_tag="J1Z28_003929"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBA7718709.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVMQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1743..2501)
                     /locus_tag="J1Z28_003930"
     CDS             complement(1743..2501)
                     /locus_tag="J1Z28_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBA7718710.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALFPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2721..3770
                     /gene="sohB"
                     /locus_tag="J1Z28_003931"
     CDS             2721..3770
                     /gene="sohB"
                     /locus_tag="J1Z28_003931"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBA7718711.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3806..4057)
                     /locus_tag="J1Z28_003932"
     CDS             complement(3806..4057)
                     /locus_tag="J1Z28_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBA7718712.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4437..7034
                     /gene="topA"
                     /locus_tag="J1Z28_003933"
     CDS             4437..7034
                     /gene="topA"
                     /locus_tag="J1Z28_003933"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBA7718713.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         1801 a         1679 c         1926 g         1680 t
ORIGIN      
        1 ggggggcgga ggcggtggtg gttggtaacc aaatcgacgt taatcgaaac aagctttaac
       61 cataaagata gtgtagtatg ttgcgcctca aagccaggcc agttaaaacg tcgagtcgtt
      121 tacttaaggc ctgaagagtt caaacagact ttactatcag tgagtcagat gtaagttctg
      181 actcattgtt gcacaaaagc atatcagacg cctgggaagg cgacaccaaa aggaagctct
      241 atgagcgaaa agctacagaa agtgctggcg cgtgccggcc acggttctcg ccgtgaaatc
      301 gaatctatta ttgaagccgg tcgtgtgagt gttgatggca aaattgctaa actcggcgat
      361 cgcgttgaaa ttacccctgg actgaaaatc cgtatcgatg gtcacctgat ttcggtacgt
      421 gagtccgctg aacaaatttg tcgcgttctg gcctattaca aaccggaagg tgagttgtgt
      481 acccgtaacg atccggaagg gcgtccaacg gtgtttgacc gtctgccaaa gctgcgtggc
      541 gcacgctgga ttgccgtagg tcgtctggac gttaacacct gtggtctgtt gttgttcacc
      601 accgacggtg aactggcaaa ccgtttaatg catccaagcc gtgaagttga acgtgaatat
      661 gccgtgcgtg tatttggtca ggttgacgac gcgaaactgc gtgatttgag tcgtggcgtg
      721 cagctggaag atggtccggc agcctttaaa accatcaagt tcagcggcgg cgaagggatc
      781 aaccagtggt acaacgttac tctgaccgaa ggccgtaacc gtgaagttcg tcgtctttgg
      841 gaagcggttg gtgtgcaggt gagccgcctg attcgtgttc gttacggtga tatcccactg
      901 ccaaaaggtc tgccacgcgg tggctggacg gagctggacc tcgcccagac taactatctg
      961 cgcgaactgg tggaactacc gccagaaacc agctctaaag tcgctgtaga aaaagaccgt
     1021 cgtcgtatga aggcgaatca gattcgtcgt gcggtgaaac gtcacagtca ggtgagcggc
     1081 ggtcgtcgtt ctggcggacg taataataac ggttaatcaa agtatgccgg acgtcatatc
     1141 cggcattttt acagattagt aatcaatccc tatctgcgct tttacaccgg catcaaacgc
     1201 atgtttgacg gggcgtaatt cacttaccgt atccgccagt tcaagaatat cccgatgaca
     1261 accacgacct gtgataatca ccgtctgttg atgtggacgt tcatttaacg cctgcatcac
     1321 ttcttccagt ggcaaatagt catacgccac catatacgtc agttcatcga gcaaaaccat
     1381 atccagtgag gaatcagcca gcatccgctt tgcatgttgc cagacttcgc ggcaggcggc
     1441 ggtatcagac tctcggtttt gtgtatccca ggtaaagccc gttgccatta cctgaaactc
     1501 aacgccgtgt ggctccagca gatttcgttc accattaggc caggtgcctt taataaactg
     1561 cacgacgccc actttttttc cgtgaccaac tgcgcgtgtt gccgtaccaa atgccgcggt
     1621 ggttttgcct tttccattgc cggtaaagac gatgataata ccgcgttcat cctgggcctg
     1681 ggccacacga gcatctactt tttctttcac tcgctgctga cgctgttggt agcgttcatc
     1741 actcattggg aaattcctgg tttacggccc ggttgggcgt caaaggtcat gccggtttta
     1801 cggcggctgt catcgcccat cagccagagg tagagcggca tgatatcagc gggggtttta
     1861 agtttctgtg gatcttcggt cgggaaggcg ctggcccgca ttgcggtgcg cgtaccgcct
     1921 gggttaatgc agttgacgcg caggcgctgc tgatattcat cggccagtac ctgcatcatc
     1981 ccttcggtgg caaatttcga cgctgcatat gcgccccagt ttgcacgccc ctgacgtcca
     2041 acgcttgatg aggtaaagac cagtgaaccg gcatccgatt tgagtaataa aggaaacagt
     2101 gcctgtgtga gcataaaggt ggcattaacg ttgacctgca tgacgtcctg ccagacctgc
     2161 ggatcttgtt cgctcattgg gcaaacatcg ccgagcaatc cggcattatg caaaacacca
     2221 tccagacgcg gataattaac ggcaatgcgc tgtgccagtt gttggcaatc ttcggacgtg
     2281 caggttagca aatcgagaat aaaccactgt ggctgacgtc cagtttcttc gtttatgtgg
     2341 ctggctacct gacgtaattt ttcttcatta cggcccaaaa gaatcactgt cgcaccatag
     2401 cgtgcatacg tcatcgctgc ttcacgacca attccatcgc tggctcccgt caccaggata
     2461 atgcggtcat tgagtaaatc ttgttttggc tggtaatgca tggctactcc tcaacgacgt
     2521 tgtctgtcgc gctgccagtg tactttatga ctttggggct ttatgcctga aacagaaggg
     2581 catttcaatc agcctggggc gataacaccg caaaatggat actttgtcat actttcgctg
     2641 caataacatc tctgcgagac ggctaaacat gcctgttgta aactgtgagc caaagcgttg
     2701 tttaaccaag gtggggactc gtggaattgt tgtctgaata tggtttgttt ttggcgaaaa
     2761 tcgttaccgt tgtgctagcg attgcggcga ttgccgccat tattgtcaat gttgctcaac
     2821 gtaataaacg ccagcgtggc gagttacgag tcaacaatct cagcgaacag tataaggaga
     2881 tgaaagaaga actggccgcg gcgctgatgg acactcatca gcaaaaacag tggcacaaag
     2941 cgcagaagaa aaagcacaaa caagaagcga aagccgcaaa agcgaaagct aaactgggcg
     3001 aggtggtaac tgacagtaaa ccccgcgtct gggtgctgga ttttaaaggc agcatggacg
     3061 cccatgaagt gaactcgcta cgtgaagaga taacggcggt actcgcagca ttcaaaccgc
     3121 aggatcaggt tgtgctccgt ctggaaagcc caggtggcat ggtgcatggt tacgggttgg
     3181 cggcttcgca gttgcagcgt ctgcgtgata aaaacattcc tttaactgtt acggtagaca
     3241 aagtcgctgc cagcggcggt tacatgatgg cctgtgtggc ggacaaaatt gtttccgcac
     3301 cgtttgctat tgtgggttcc attggggtgg tggcgcaaat gcccaacttt aaccgcttcc
     3361 tgaaaagcaa agatattgat atcgaactgc acaccgccgg gcagtataag cgcacgctga
     3421 cgttgctggg tgaaaatacc gaagaagggc gggagaaatt ccgcgaagag ttgaacgaaa
     3481 cgcatcagtt atttaaagat tttgtgaagc gtatgcgtcc gtctctggat attgaacagg
     3541 tggcaacggg tgaacactgg tacggacaac aggcagtaga gaaaggcctg gttgatgaaa
     3601 tcaacaccag tgatgaagtt attcttagcc tgatggaagg ccgggaagtg gtcaatgtac
     3661 gctatatgca gcgtaaacga ctcattgacc gattcaccgg cagcgcggca gagagcgccg
     3721 atcgattgtt gttacgctgg tggcagcgtg ggcaaaagcc gttgatgtaa aagacaaacg
     3781 cgaggataag acctcgcgtt ttgctttaat caaccaagtg atatttttct gaaagcacat
     3841 gggccaggtg tttgaacata ttaaacaccg cggtgctttt ggcagttggt aatccttgtt
     3901 catctaaaaa gtagtcgccg gtaaatacca gcacgccatt acgctgcgtg acgccggtgg
     3961 cttcaatccc tgcgagcgta tcctcatgct cacgaatgat tttgttggcc tctttcaaca
     4021 gcgtttcgcg gtcgatgggt tgagtctctt tgttcattat ttactcctta aacaaggaca
     4081 ttagtctacg ccaggcatgg cttgcagaca aatataccac gctggtggca agagcgcctt
     4141 actggcaact ttggattttg catgctaata aagttgcgta tcggatttta tcaggtacag
     4201 tgtgacgctt tcgtcaatct ggcaatagat ttgcttgaca ttcgaccaaa attccgtcgt
     4261 gctatagcgc ctgtaggcca agacctgtta actcagtcac ctgaattttc gtgagcagag
     4321 tcacgacaag gggttgatat ccgcagagag cgagtccata tcggtaactc gttgccagtg
     4381 gaaggtttat caacgtgcga cgcattcctg gaagaatcaa attaggtaag gtgaatatgg
     4441 gtaaagctct tgtcatcgtt gagtccccgg caaaagccaa aacgatcaac aagtatctgg
     4501 gtagtgacta cgtggtgaaa tccagcgtcg gtcacatccg cgatttgccg accagtggct
     4561 cagctgccaa aaagagtgcc gactctacct ccaccaagac ggctaaaaag cctaaaaagg
     4621 atgaacgtgg cgctctcgtc aaccgtatgg gggttgaccc gtggcacaat tgggaggcgc
     4681 actatgaagt gttgcctggt aaagagaagg tcgtctctga actgaaacaa ctggctgaaa
     4741 aagctgacca catctatctc gcaaccgacc ttgaccgcga aggggaagcc attgcatggc
     4801 acctgcggga agtgattggt ggtgatgatg cgcgctatag ccgagtggtg tttaacgaaa
     4861 ttactaaaaa cgcgatccgc caggcattta acaaaccggg tgagctgaat attgatcgtg
     4921 ttaatgccca gcaggcgcgt cgctttatgg accgcgtggt ggggtatatg gtttcgccgc
     4981 ttctatggaa aaagatcgct cgtggtctgt ctgccggtcg tgtgcagtcg gtggcggtcc
     5041 gcctggtggt cgagcgtgag cgtgaaatta aagcgttcgt accggaagag ttctgggaag
     5101 tcgatgccag cacgaccacg ccatctggtg aagcgttggc gttgcaggtg actcatcaga
     5161 acgacaaacc gttccgtccg gtcaacaaag aacaaactca ggctgcggta agtctgctgg
     5221 aaaaagcgcg ctacagcgtg ctggaacgtg aagacaaacc gacaaccagt aaacctggcg
     5281 ctccttttat tacctctacg ctgcaacaag ctgccagcac ccgtcttggt tttggcgtga
     5341 aaaaaaccat gatgatggcg cagcgtttgt atgaagcagg ctatatcact tacatgcgta
     5401 ccgactccac caacctgagt caggacgcgg taaatatggt tcgcggttat atcagcgata
     5461 attttggtaa gaaatatctg ccggaaagtc cgaatcagta cgccagcaaa gaaaactcac
     5521 aggaagcgca cgaagcgatt cgtccttctg acgtcaatgt gatggcggaa tcgttgaagg
     5581 atatggaagc agatgcgcag aaactgtacc agttaatctg gcgtcagttc gtagcctgcc
     5641 agatgacccc agcgaaatat gactccacga cgctgaccgt tggtgcgggc gattttcgcc
     5701 tgaaagcacg cggtcgtatt ttgcgctttg atggctggac gaaagtgatg cctgcactgc
     5761 gtaaaggcga tgaagatcgt atcttaccgg cagtcgataa aggcgatgct ctgacgctcg
     5821 ttgaactgac accagcccag cactttacca agcctccagc ccgtttcagt gaagcatcgc
     5881 tggtcaaaga gctggaaaaa cgcggtatcg gtcgtccttc tacctatgcg tcgatcattt
     5941 cgaccattca ggatcgtggc tatgtgcgag tagaaaatcg tcgtttctat gcggaaaaaa
     6001 tgggcgaaat cgtcaccgat cgcctggaag agaatttccg cgagttaatg aactacgact
     6061 tcaccgcgca gatggaaaac agcctcgacc aggtggcaaa tcacgaagca gagtggaaag
     6121 ctgtactgga tcacttcttc tcggatttca cccagcagtt agataaagct gaaaaagatc
     6181 cggaagaggg tggtatgcgc ccgaatcaga tggttctgac cagcattgac tgcccgactt
     6241 gtggtcgcaa aatggggatt cgcacagcga gcaccggggt attccttggc tgttctggct
     6301 atgcgctgcc gccgaaagag cgttgcaaaa ccaccattaa cctggtgccg gaaaacgaag
     6361 tgctgaacgt gctggaaggc gaagacgctg aaaccaatgc gttgcgcgca aaacgtcgtt
     6421 gcccgaaatg cggtacggcg atggacagct atctcatcga tccgaaacgt aagttgcatg
     6481 tctgtggtaa taacccaacc tgcgacggtt acgagatcga agagggcgaa ttccgcatta
     6541 aaggttatga cggcccgatc gttgagtgtg aaaaatgtgg ctctgaaatg cacctgaaaa
     6601 tggggcgttt cggtaaatac atggcctgca ccaacgaaga gtgtaaaaac acacgtaaga
     6661 ttttacgtaa cggcgaagtg gcaccaccga aagaagatcc tgtgccatta ccggagctgc
     6721 cgtgcgaaaa atcagatgcc tatttcgtgt tgcgtgacgg tgctgccggt gtgttcctgg
     6781 cggccaatac cttcccgaaa tcgcgtgaaa cgcgtgcgcc gctggtggag gagctttatc
     6841 gcttccgcga ccgtctgccg gaaaaactgc gttatctggc cgatgcgcca cagcaggatc
     6901 cggaaggtaa taagaccatg gttcgcttta gccgtaaaac caaacagcaa tatgtctctt
     6961 cggaaaaaga cggaaaggcg actggctggt cagcatttta tgttgatggc aaatgggttg
     7021 aagggaaaaa ataaccttta attccgtctg gtttttataa acaaagggtc gcgaaagcgg
     7081 cccttt
//
LOCUS       DADSZD010000197         6992 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.199, whole genome shotgun sequence.
ACCESSION   DADSZD010000197 DADSZD010000000
VERSION     DADSZD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.199"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            109..1887
                     /locus_tag="J1Z28_003934"
     CDS             109..1887
                     /locus_tag="J1Z28_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBA7718714.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDENRVQFVNQTALKTLGVVQNNIIGKPVRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANASDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KEVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1927..2406)
                     /gene="xdhC"
                     /locus_tag="J1Z28_003935"
     CDS             complement(1927..2406)
                     /gene="xdhC"
                     /locus_tag="J1Z28_003935"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBA7718715.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLATWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2403..3281)
                     /gene="xdhB"
                     /locus_tag="J1Z28_003936"
     CDS             complement(2403..3281)
                     /gene="xdhB"
                     /locus_tag="J1Z28_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBA7718716.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELQGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLELHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3292..5589)
                     /gene="xdhA"
                     /locus_tag="J1Z28_003937"
     CDS             complement(3292..5589)
                     /gene="xdhA"
                     /locus_tag="J1Z28_003937"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBA7718717.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPEIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITSPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFIGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVHVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLIERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6003..6758
                     /locus_tag="J1Z28_003938"
     CDS             6003..6758
                     /locus_tag="J1Z28_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBA7718718.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6837..6910
                     /locus_tag="J1Z28_003939"
     tRNA            6837..6910
                     /locus_tag="J1Z28_003939"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6869..6871,aa:Gly,seq:ccc)
BASE COUNT         1738 a         1665 c         1784 g         1805 t
ORIGIN      
        1 tcttatgttt ttatcaaagc caatttcgcc tgagttaata tcatcatgat ctgtgaacca
       61 tcaacgtctt caatgatcga cttttaagta agtctggaag gtaaagctat ggagcttgcg
      121 actacgcagt cagtattgat gcaaattcaa ccgacaattc agcgttttgc cagaatgctt
      181 gccagcgttt tgcagcttga ggttgagatc gttgatgaaa acttgtgtcg cgtcgccgga
      241 acgggcgcgt atgggaagtt tcttggccgc cagttgagcg gcaactcacg cctgctccgc
      301 cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt ctcgcttcga tcccctttgt
      361 gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat ttctgggtac gcctgtcatt
      421 ttacaggatc gttgtgttgg ggtgataagt ttgattgccg ttacccacga gcaacaagaa
      481 catatcagtg ataatttacg cgaattttct gattacgttc gccatatatc caccattttt
      541 gtttcgaaac ttctggagga tcaggggcca ggagataaca tcagtaaaat attcgcgacc
      601 atgatcgata atatggatca gggcgtttta gttgttgatg acgaaaatcg tgttcagttt
      661 gttaatcaga cagctttaaa aacactcggt gttgtacaaa ataatattat tggaaaacct
      721 gttcgtttca gaccattaac atttgagagt aattttaccc atggtcatat gcaacatatt
      781 gtttcgtggg atgataaaag tgaattaatc attggtcagt tgcataacat ccagggccga
      841 caactatttt taatggcgtt tcaccaatcg cataccagtt tttctgtagc aaatgcatct
      901 gatgaaccgc atattgaaca attggttggc gagtgccgtg ttatgcggca attaaaacga
      961 ctcattagcc gtattgcacc cagcccatcc agcgttatgg tggttggtga aagcggcacg
     1021 ggtaaagaag tcgtcgcccg tgcaatccat aagttgagcg gaagacggaa taaacccttt
     1081 attgctatca actgtgccgc gattccggaa cagcttctgg agagcgaact gttcggttat
     1141 gttaaaggcg catttactgg cgcttctgcc aacggtaaaa cagggttgat tcaggcggcg
     1201 aatacgggca cgctgtttct tgatgaaatt ggtgatatgc cattaatgtt gcaggctaaa
     1261 ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg gtgccagtag cccaatacaa
     1321 gtcgacatcc gcatcatttc tgcaactaat cagaatttgg cccagttcat tgccgaaggt
     1381 aaattccgcg aagatctctt ctaccgactt aatgttatcc cgataactct tccaccgctg
     1441 cgtgaacgtc aggaagatat tgaattactg gtgcattact ttttacatct gcatacccgt
     1501 cgtctgggat cggtttatcc tggcattgct cctgatgtcg tcgaaatatt gcgtaagcat
     1561 cgttggcccg gaaacctgcg cgagttaagc aatttgatgg aatatctggt taacgttgtt
     1621 ccttcaggtg aagttatcga cagcacgcta ttgccgccaa atctgctgaa taatggcaca
     1681 acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc tgtcactcga tgatgcgggc
     1741 ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg aggcgctttc acgtcataac
     1801 agcaagaagg aagttgctga tgaactgggc atcggcattg ctacgctcta tcgcaagatt
     1861 aagaaatatg agttgttaaa cacataacct ctgagaatcc ggcctgaatt caggccggat
     1921 atccttttac ttcgttttct cgcaatccag aactgtattt acaatcatct gataccccgt
     1981 gcagcgacaa agatttcccg ccagtccacg acgaatttcc gtaatggtta atggtttttc
     2041 gcgtggtttt gccagcatcg ccgtggtagc cataatcagg cccggcgtac aaaacccgca
     2101 ttgcactgca ccagatttcg cataagccag ttggacatga gaaagtttac cgcctttcgc
     2161 ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag gtcgcaagga ataagcaact
     2221 gtctatcgca gtgccgtcga ccagcaccgt acaggcaccg cattcgccta cgcagcaacc
     2281 ttgtttgaca ctaagaagcc cttgttctcg gagtagttcc gaaagcggca ttcctggcgc
     2341 ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg atggtaattg tttcgctgtg
     2401 attcattgca attttccccc cgccgcggcg acggcttcgc taatcacttt tttggtcatc
     2461 gtctggatga gatgcagacg aaactcttta ctggcccgcc atgaagaacg cggggcgaca
     2521 tcttgcagga ctgattcgct gatggcttcc agcgtttgca ggtttaatgg cgcattttgt
     2581 gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc aaatgccagg
     2641 cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg cggcgcagcc aattgttgaa
     2701 atatccattg cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg ttctttcggc
     2761 tgtggcggaa aatgaaaggc gacaaggatt tcgtcatgct caagagacac tttgcccggc
     2821 ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc gtggggagtg gagctccagt
     2881 ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc tggtggcacc gttgcaaata
     2941 tttccgccgt aggtggcgac attacggatc tgcggcccgg cgattgatgc agccgcagca
     3001 cataacgccg ggagattgcg ttggattacg ggatcttcaa tgagctgagt aaatgttgtc
     3061 gcagagccga ttcgcagcgc gccatcttcc gcctgtgtta ttccctgaag ctctgccaga
     3121 ttgtggatat caacaatatg gcgatagcgg tcattgtgat ggtgaagctg tatcagtacg
     3181 tcagtgccac cggcaagcaa tttggcctgc ggattgtcag ccagcagggt gatggcatcg
     3241 gcaagggtgg ttgcgcgatg gtaagaagca aaatcaaaca tgatgttatc ctcaaatcaa
     3301 tcctgccaga tggaattctt catataatcg ttttggcgtt agcggcagtg tattgattgc
     3361 aacaccggta gccatcttca ccgcgttacg aatagcagcg gctacaggaa ttatgggggg
     3421 ctcacccagt gacttatgtc cgtatgctga ttgcggctca ttgatttcga cgaacgcgct
     3481 ttccagttgt ggcagatccg gcatggtcgg cattttgtaa tccagcagat tggggttacg
     3541 gaccacgccg cttttcgcat cgatgatcat ctcttcaaat agcgcccagc caatgcccat
     3601 tcccattccg ccgtgtacct gaccttctgc cagcagcgga ttaagaatat ggcccgaatc
     3661 atgaacgttg aggatgcggt tgatggtgac tttgcacagt gcaatatcga ccgtcagatc
     3721 aacaaaggta cagccaaacg ccggtgggtt agtggtggtt ttgatggagc tttcggcaga
     3781 gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt ttaacgacat
     3841 taacggttct tccggtcttt caatcagcac gatatggcct tttatcaggg tcagattcat
     3901 cgctgactga tgtagcatga ctgcggcgtg agcgatgatt ttctctttta ataacagtgc
     3961 cgcactgcgc agcgcaggcg cggcaacata gctctgacgt gaggcaaatg cgccggggtc
     4021 gaatggtgta acgtcggtat cttgggttga aataacgtga acatcgctga ccggaactcc
     4081 cacggtttct gccaccattt gcgagaacac ggtgtcggca ccctggccga tttccgtcgc
     4141 gccgctttgc acgttgatgg ttccatcctg attcatcaac aggcgcgcgc ctgctatttc
     4201 tacgccgaca ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa cgccacgacg
     4261 taaattgcct tgctggttct ggcactctgc acggcgtttt tcccattcaa agattttccg
     4321 gcctttttca agacactccg gtaaccctgc gctgtaaata cgttttcccg tgagcggatt
     4381 agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa tacctaacgc
     4441 tgtcgcggca tcatcaagca tagactcaac ggcaaatacg acttgtggcg cgccataacc
     4501 acgcatcgca ccagccgagg ggaggttggt atagcaggtc tttgaactgt aagcgtaggc
     4561 acaacgagga taaaggtaag cgactttatt ccccccagca gaagcaatgg agtgcccgtg
     4621 agatgcataa gcgccggtgt tagacagaac atccagacta taacctttca atgttccgtc
     4681 gcggttcacg cccatttgcc cgtcaatagt aaaagcgtgg cgggtacggg ttgcgaggaa
     4741 acactcttca cggctaaggg aaactttcac cggaatgccg ccaagtttgc tggtcaggaa
     4801 tgccgccatt ggctcttcca gtacatcctg tttattacca aaaccgccac cgataaacgg
     4861 tttgatgact cgtacgcatg accagggaat atccagcgcc tgaccaacca cgcggcgaac
     4921 aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc gagtcatcct ccatccatgc
     4981 cagcgatgtc acgctttcca tatgacaatg ttgaataacg ggagtctgat agtgcccctg
     5041 tacctggtag tcggcggcat cgattgtttg ttggacatta cccgtcgaca tcgtgctttg
     5101 tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct tctgccagcg ccgcttctgg
     5161 cgaggtgata acgggtaatt cttgccactc aatgctgacc aattgcgccg ctttttctgc
     5221 cgtgagttca tcgcgggcca cgacgatggc aacggcgtcg ccatgatgac gaacatggcg
     5281 agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg tcaagtgtcc aggcatgccc
     5341 tgccgtggcg aatgggattt ctggcacatc ttcccaggta aaaatcgcca ggacgcccgg
     5401 caaactcctg gcttgttcat cattaatact tacggcataa ccatgtgcga taggactacg
     5461 tacatatttc gcgtaacaca tgcccgccat aacataatcg tcagtatatc gtgcccgccc
     5521 ggtgacctta gcaatggcat cgacgcgcat gcatgattca cccgtagcgg ttgcttcccg
     5581 cgcttccata gaaatcccct cggtttgaac atttggttct tatggcaaag atttagtctt
     5641 agcaggcaag aattacgcca gaaacgacta tatgcgtgag gggaggaagg aagtgtgatg
     5701 acgttaaaaa aacacagata tgatacgaaa caggactctg aatagggaca gtgatacgta
     5761 tcaatatgag tggcgataaa atcaatgtga taaattagga ttattacgca tcaaggtggg
     5821 aaatagggta aaagtcagga acttcgatac cactacagca aatattttgc tgatgatact
     5881 tccctttgcc aaatgaaatt aacctctttc tctattacat cgtttttcca gaagcaagag
     5941 attgggtatc aagaggctgg ctgctatgat atggcgtctt gttttttaag cgaggaaaga
     6001 ttttgagtgc gggacgcctg aataaaaaat ctctgggtat cgtgatgttg ttatcggttg
     6061 ggctgctttt ggcgggctgt tcgggtagca aatcatccga tacaggaacg tattccggct
     6121 ccgtttacac cgtgaaacgg ggggatacgc tatatcgtat ttcgcgcacc acgggaacca
     6181 gcgtaaaaga actggcgcga ctgaacggca tttccccccc ttacaccatt gaagtaggtc
     6241 agaaactaaa actgggtggg gcgaaaagta gcagtagtac acgtaaatca accgccaaat
     6301 caacgaccaa aaccgcatcg gttacaccgt catcagcggt accgaaatct tcctggccgc
     6361 cagtagggca acgttgttgg ttatggccaa cgacagggaa agttatcatg ccgtattcga
     6421 cagcagatgg cggcaataaa gggattgata tctcagctcc acgaggtaca cctatttacg
     6481 ccgcgggtgc aggaaaggtg gtgtatgtgg gtaaccagct gcgtggctac ggtaatctca
     6541 tcatgattaa acacagtgaa gattacatta cggcttacgc tcataatgac acgatgctgg
     6601 taaataatgg gcaaagcgtg aaagctgggc aaaaaatcgc cactatgggg agcactgatg
     6661 cggcatctgt tcgcctgcat ttccagattc gttaccgtgc aacggcaatt gatccgctac
     6721 gttacttgcc gcctcagggc agcaagccaa aatgctgatg gtgaattaat cagcagtcag
     6781 cagcgtggta cttgctaagg agagcgtaag gtttataatg ccttacgcat ctcgaagcgg
     6841 gcgtagttca atggtagaac gagagcttcc caagctctat acgagggttc gattcccttc
     6901 gcccgctcca atttatcttc acatcatcta cctgccattt ttgttgtatc tgattttcta
     6961 tattatctta attcaagcgc ttcagacttt tc
//
LOCUS       DADSZD010000198         6988 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.200, whole genome shotgun sequence.
ACCESSION   DADSZD010000198 DADSZD010000000
VERSION     DADSZD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.200"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(50..832)
                     /locus_tag="J1Z28_003940"
     CDS             complement(50..832)
                     /locus_tag="J1Z28_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HBA7718719.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQQATLKQR
                     TEKAARMAGRMPAGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRHA"
     gene            1479..2717
                     /locus_tag="J1Z28_003941"
     CDS             1479..2717
                     /locus_tag="J1Z28_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HBA7718720.1"
                     /translation="MEVFMKDVSILNEMIFSASTEDELQEKFEDIYHRINNESEYDDS
                     ESVEFLPLPRLNSRSERSEVQDIISVVIKSGCFTSGPYIGKVEETLRLFYGAHTCIAT
                     SSGTDALKIALKSVGVEPGDEVIVPLNSFAATENAVMAVGAVPVFANIDSSFNMLPDE
                     VDRLRTSKTKAVLPVCLYGSCMYIDAIHRIARKADIPVIVDAAQCFGIRSLISHCDLL
                     ALSFNPFKNIGSLGKSGAVLTLSPELARLARQYSYHGFAEGKKNIKAQNWGLNSRMDN
                     LQAATLSVKLQHFENNAKKRCLLAARYHLLLSDLSHNIILPTETHQNTWHLFPVLLCK
                     GERDSLFAFAREKGVELDIYYPVLSHCGEHPLATGYSRREQFRDSEKIHSALLHLPLH
                     NHMSLQEQNIVIEVLHDYFK"
     gene            2701..3531
                     /locus_tag="J1Z28_003942"
     CDS             2701..3531
                     /locus_tag="J1Z28_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HBA7718721.1"
                     /translation="MTILNKNWPVEFPFPTVIRTVVCSDLDETYIPSDSDKKKLGGVV
                     QLESYITSHAEKKGILAGWITGTNLDSAWRKSRGYISRSPHFICCSLGTEFYWVKNGR
                     LIPSETWAERISRSGYSRQNVNCLVKILMEKEIPLLKQPEDYQGPFKVSYYYPESPSI
                     ASDFATIQELADERRIRVVFTRCNPAAGDPPSCYDVEFIPLCCGKDQAVSFLMEETGL
                     PKEAIIAFGDSANDFAMFAQAGSGYLVGNADPFAVSQYGNRLDSAYCHGILSVLKELQ
                     "
     gene            3531..4547
                     /locus_tag="J1Z28_003943"
     CDS             3531..4547
                     /locus_tag="J1Z28_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7718722.1"
                     /translation="MIIGIVGCGGIGRAHVNALCLNPLVTGLALYDVSPDNVRQLSEI
                     SSLPVTNCQDLEQLAGVSQGFIICTPNHLHVSVAEEVLRYKQLPFVCEKPLSTDLNSA
                     RYLQEIAPKGSIVSFNYRYNPVIDMIMRLRNERKLGELHFFSAEFNKNSALTRHHLTW
                     RDSAQQSKSSGALGDLSCHLLDLFCFIGESPVVVHGIKTVKGTRVGTKSDGQVEVDDN
                     GYVMGSSEKGAYFRVHASKSETDQNLGLHIQLVFENGEIRYSTHHENRLLLILFNDTN
                     TETIGFDALKRLPDPPRELPFWSDSFIHLHDDWCALIKYGHSSPKLADLSSGLHIQEI
                     IEAF"
     gene            4549..4818
                     /locus_tag="J1Z28_003944"
     CDS             4549..4818
                     /locus_tag="J1Z28_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718723.1"
                     /translation="MDTILLTGLFAAFFTTFAFAPQSIKTIRTRNTEGISVVMYIMFL
                     TGVISWIAYGIMRSDFAVLIANIVTLFLAAPVLVITLINRRKKHV"
     gene            4815..6347
                     /locus_tag="J1Z28_003945"
     CDS             4815..6347
                     /locus_tag="J1Z28_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004172011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HBA7718724.1"
                     /translation="MNIAIIGAGPAGIISARNAIKAGHSVVLFEKNTRIGGIWNPWSG
                     GAYRNACMQNSRYTFHYTGFPPGDIDEFPGVEQVFRYLSAVAGEDALRESIRLNTEVV
                     SLRKDAGHWVIRCASEGKDTEDIFDRVIIATGELWQPRRPPLPGEENFSGTLITSRDY
                     QEPEAFKGKNILIIGGGVSGADIASDLVPFARSVSLSVKKMGLYLPRQFPTGPNDMMH
                     SYLGRCLLSQMNYEDFIGYLDTMMPDYMQAYRASGLLPDMANNNAVHVNEKIIPNVAA
                     GLIKVKPQAERFTGEGAIKFVDASQEKYDVIITCTGYEMPDYSFIQGFDRTQLYEHFF
                     WTEDPSLAVINPPVDTAGFGAAFPYFDIISQWVMNVFSGKTSLPEKEAMRKWCAEHMA
                     SLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMVKPAYLATPPAFPEHGM
                     MDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALEIDPRQSISVHLPYSQT
                     YL"
     gene            complement(6379..6831)
                     /locus_tag="J1Z28_003946"
     CDS             complement(6379..6831)
                     /locus_tag="J1Z28_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004168570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBA7718725.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISITLGD
                     SVTVGSGAIILSPYICSGAVIGAGSVVVKPIENKGIYAGNPARLLRIL"
BASE COUNT         1818 a         1707 c         1658 g         1805 t
ORIGIN      
        1 cagtagtctc ttttttatag aagaaatcac aattcataat tgtgacttat tatgcatgtc
       61 tgtttttcag cattgtgacc gcatcggact caggcatcaa aaactgcacc cggtgccgtg
      121 cagccacatc cagtgcaaac accttcgtgt agacctccgt tgagctgatg gacttatgcc
      181 ccatcaggct ctgcaggacc ttcagcggta taccggcata cagcatgtgc atcgcatagg
      241 aatggcggaa cgtatgtggc gtgaccggga cagagaacgt cacaccatca gcggcagcgg
      301 cggcaaccgc ctccccaatc caggtcctga ccgttctgtc cgtcacttcc cagatccgcg
      361 ctttctctgt cctgcctgtt cgtttattac gccgttccat ggggattttc agtgtggcca
      421 ccatcgtctg cagctggctg acgtaccagg cgtcggagag cggaaccagc cggtgagtct
      481 gctgaccggc gggcattctt cctgccatcc tggcggcttt ttctgtccgc tgcttcagag
      541 tggcctgctg cacaaacgga tacggaggcg caagcgaaaa atccccccgc gtcagcgcca
      601 gtgcttcatt aatgcgcgca ccggtgttcc acagtgtggc cagcagcatc ttgcggcgca
      661 gatccgggac gtaatggagc agggcgctca cttcaggggc cagcaggtat tttggcagtt
      721 catcatgaac catcgacatc tggcggagtg ccagcgctgc cggataatcg atggcaacag
      781 gaagctgagc ggcatcccgg gcatggtgga tcggtgccgg caggtgctgc atatcagtgt
      841 tcactccacg gggaaacgtc gggcaggttt tccacaaccc ggtactttcc cggataacgg
      901 agctggttac cgccacgcgt ccagacggtg acaacaaaac ccccgccctg gttgttatag
      961 tgtcatcagc agctgaatcc ggcaccacat gttgtgctga cgggaggtgg cgttgtgtgg
     1021 ggggagagga agatcctgaa caatgagttt tcggatctca ggaagggaaa aaacagattt
     1081 ttgcaggata agcactaaaa acggtgaatt gttgtgatta ggggattatt gcaatcagaa
     1141 caataactgg aattgaaaag gttatattca tcaataaata gcagagtcac agacctgctg
     1201 aaagccagac catgaagata ctacccgagg aagatccatt tcaaatattc ggatacacag
     1261 gcaactgtgg cgacaattgt aatgacatta accatatcgg cttctctgaa gcattggtta
     1321 tggcaaccac gttatcaata cctttccatc gttttcagta aatgtcacta acagaccgat
     1381 attaccggac ctgtaagttg tctgtgacaa atatcaccaa tttgtaacta aaatgtgaaa
     1441 atacggttta tctgtgcttt atttttctcc gacataaaat ggaggtcttt atgaaagatg
     1501 tcagtatttt gaatgaaatg atcttcagtg catcaacaga agatgaacta caggaaaaat
     1561 ttgaagatat atatcaccgt atcaacaacg agagtgaata tgatgactcg gagtcagtcg
     1621 aatttctgcc acttccaaga ctgaattccc gttctgaacg ctcagaagtg caggacatca
     1681 tttcagttgt aattaaaagc ggatgtttta ccagtggccc ctatattgga aaagtagaag
     1741 agacattaag attattttat ggtgctcata cctgtattgc caccagtagc ggcaccgatg
     1801 cgttaaaaat agctcttaaa tctgttggag tggaacccgg agatgaagtc atcgttccac
     1861 tgaactcctt tgcagctacg gagaatgccg tgatggccgt gggagccgtt ccggtcttcg
     1921 ctaatattga cagttctttc aatatgcttc cggatgaagt ggatcgcctc agaacttcaa
     1981 agacaaaagc cgtgttaccg gtctgccttt atggctcctg catgtacatt gatgccattc
     2041 accggattgc ccgcaaagcc gacattcctg ttatcgtgga tgcagcacag tgctttggta
     2101 tcaggtcact catcagccac tgtgatctgc tggcgttgag ttttaatcca tttaagaata
     2161 ttggttctct tggtaaatcc ggtgcagttc tgaccctttc tcctgaactg gcacgcctgg
     2221 caaggcaata ttcatatcac ggatttgctg aaggtaaaaa gaatattaaa gcccagaact
     2281 ggggattaaa cagtcgtatg gataatcttc aggctgccac gcttagtgtc aaacttcaac
     2341 attttgaaaa taatgcaaaa aaacgctgcc ttcttgcagc ccgttaccat cttttactgt
     2401 ctgacctgtc acataacata atacttccga cagagaccca tcagaataca tggcaccttt
     2461 tcccggtact gctctgcaaa ggcgaacgtg attctctgtt cgcctttgct cgtgaaaaag
     2521 gagtggagtt agatatttac tatcccgtcc tttcacattg cggagagcat ccattggcaa
     2581 ccggatattc ccgtcgagag cagttccgtg attctgaaaa aatacattct gcattacttc
     2641 atctgccact gcataaccat atgtcgctcc aggagcaaaa catcgttatc gaggtactgc
     2701 atgactattt taaataaaaa ctggcccgtc gaattcccgt tcccgacagt aatccgcact
     2761 gtggtgtgca gcgatcttga tgaaacttat attccttcgg acagtgataa aaaaaaactg
     2821 gggggggtcg ttcagctgga gtcctatatt acatcccatg ctgaaaaaaa ggggattctc
     2881 gctggctgga tcacgggaac aaatctggat tcagcctggc gaaaatccag gggatatatc
     2941 tcgcgaagcc ctcactttat atgttgcagt ctcggcaccg aattttactg ggtgaaaaat
     3001 ggccgtctca tcccctctga aacctgggca gaaagaataa gccgttcagg ctacagccgt
     3061 cagaatgtca actgtctggt taagattctg atggaaaaag agatccccct tctgaaacaa
     3121 ccagaggatt atcagggccc ctttaaagtg agctattact accctgagag cccatctatt
     3181 gccagcgatt ttgccaccat tcaggaactg gctgacgaac gacgaattcg tgttgtgttt
     3241 acccgctgta acccggcggc tggcgatccc cctagctgtt atgacgttga atttatcccc
     3301 ctctgttgtg gcaaggatca ggctgtgtct tttctgatgg aagaaaccgg actgccgaaa
     3361 gaagccatta ttgcctttgg tgacagtgcg aatgattttg ccatgtttgc acaggccgga
     3421 agtggctatc tggtgggtaa tgccgacccg tttgcagtca gccagtacgg aaacagactg
     3481 gacagtgctt actgccacgg gatactgagc gttcttaagg agctacagtg atgattatcg
     3541 gaattgtcgg ctgtgggggg attggccgtg cccatgtcaa cgcactttgt ctcaatccgc
     3601 tagtaactgg tctggccctg tacgatgtga gtccggacaa cgtccgacag ctcagcgaaa
     3661 taagctccct gccggtaaca aactgccagg atctggagca gcttgccgga gtcagtcaag
     3721 ggttcattat ctgcacacca aaccatctcc acgttagcgt ggcagaagaa gtgcttcgct
     3781 acaaacagct cccgtttgtg tgtgaaaaac ccctctcaac agatctcaac tctgcacgat
     3841 atctacagga gattgctcca aaaggcagta tcgtgtcttt taactaccga tataaccccg
     3901 tcatcgacat gataatgcgt ctgagaaatg agaggaaatt aggtgaactg catttcttct
     3961 cagcagaatt taataaaaat tctgcactga cccgccatca cctgacctgg cgagactccg
     4021 cacaacagag caaaagcagc ggtgctctgg gcgatctatc ctgtcatctg cttgatttat
     4081 tttgttttat aggggaaagc ccggtagtgg ttcatgggat caaaacggta aaggggacac
     4141 gggtgggtac aaaatcagat ggtcaggttg aagtggatga taacggctat gtaatgggca
     4201 gttcagaaaa gggagcttat ttcagagttc atgccagtaa atcggaaaca gatcagaatt
     4261 tgggactgca tatacagctt gtatttgaaa atggtgaaat cagatattca acccaccatg
     4321 aaaatcgcct gctcctgatt ctatttaatg atacgaatac ggaaacaatt ggctttgacg
     4381 ctctgaaacg cttgcctgat ccaccacggg agcttccatt ctggtctgat tcgtttattc
     4441 atcttcatga cgactggtgt gctcttatta aatatggtca ctcgtctcct aagctggcgg
     4501 acctgtcatc cggccttcat atacaggaaa taattgaggc attctgatat ggataccatt
     4561 cttttaaccg ggctgtttgc tgcatttttc acaacgtttg cgttcgctcc ccagagcata
     4621 aaaactatca ggacacgcaa taccgaaggt atctccgttg ttatgtatat catgttcctg
     4681 acaggtgtaa tctcatggat agcgtatggc attatgcgat ctgattttgc cgtactgatc
     4741 gccaatattg tcacactgtt tctggctgca ccagtgcttg taataacact tatcaaccgc
     4801 aggaaaaaac acgtatgaat attgcaatca tcggcgcggg tcctgccggc atcatttctg
     4861 cccgtaatgc gattaaagca ggtcactccg tggttctgtt tgagaagaat acccgaattg
     4921 gggggatctg gaacccctgg agtggtggcg cttaccgtaa tgcctgtatg cagaactctc
     4981 gttatacatt tcattatact ggctttcccc ctggcgatat cgatgaattt ccaggagtgg
     5041 aacaggtatt cagatatctt tcggccgtgg ccggagagga tgccctccgt gagtcgatcc
     5101 gactgaatac tgaagttgtt tcactcagaa aagacgccgg acactgggtg atccgctgcg
     5161 cttctgaagg aaaagacacg gaagacattt ttgaccgggt catcattgca acaggtgaac
     5221 tctggcaacc ccgccggccc cccctgccag gtgaggaaaa cttctccgga acgttgatca
     5281 cgtcgagaga ttatcaggag ccagaggcat ttaaaggaaa aaatatcctc atcattggcg
     5341 gcggtgtcag cggtgcggat attgcctcag accttgttcc ctttgccaga agcgtaagtc
     5401 tgtccgtcaa aaagatggga ctttatctgc cgagacaatt cccgactggc ccgaatgaca
     5461 tgatgcactc ctatctgggc aggtgtctgc tgagccaaat gaattatgaa gattttatcg
     5521 gttatctcga caccatgatg cctgactaca tgcaggccta ccgagcatcc ggtctgttgc
     5581 ccgacatggc gaacaataac gcggtgcatg ttaacgaaaa aatcataccg aatgtggccg
     5641 ccggtctgat aaaagttaaa ccccaggcag agcgtttcac cggagaaggc gctattaagt
     5701 tcgtcgacgc atctcaggaa aaatatgatg taataataac ctgtacaggc tatgaaatgc
     5761 ctgattactc gttcattcag ggatttgaca gaacacagct ctatgagcac tttttctgga
     5821 cagaggaccc ttctctggcc gtcattaatc ccccggtgga taccgccggt ttcggggctg
     5881 cgtttcccta ttttgatatc atcagccagt gggtaatgaa cgtattctcc gggaagactt
     5941 cccttccgga aaaagaagct atgcgcaaat ggtgtgctga gcacatggct tcacttcatg
     6001 ttaagcgatt ttatgacagc tggctggaaa ccatccggat tgggttgctg tcaggtcttc
     6061 tcccggaccc ggcccgcgac ttttcccgat actggaacat catcagcagt atggtcaaac
     6121 ctgcctatct ggccactcct cccgcatttc cggagcatgg tatgatggac agcctgtttg
     6181 atttccggat cgccagaata cgcatactgt cagggctggg aaacgatgct ctcggttatt
     6241 tactgaaaaa aggtgatatc actgacgcag aataccgggc ggccctggaa atcgatcccc
     6301 ggcagtcgat ttctgtacac ctgccgtatt cacagaccta cctctgataa aaagggccag
     6361 taacggccct tttctttttc ataatattct gagcagtctt gccgggtttc ctgcataaat
     6421 gcctttgttc tcaatcggtt ttaccacaac tgaacccgca ccaataacag ctccgctgca
     6481 tatatacgga gacagaataa tggctccact gccaacagta accgaatccc ccagggtaat
     6541 gctgatccag ttatccggtg acggatctgg tgctcctgaa cgaaacagat cgttggcgaa
     6601 cgtcacgtta tgaccaatga agcagttctc ccccagtgtt acgttctcac aaataaatgt
     6661 gtgcgactgt atccggcttc cactaccaat gacacagcct ttctgtatct caacgaaggg
     6721 gccgacaaac acgttgtccc tgagttcaca ttcataaatg ttgaccggtt tgacaatacg
     6781 gacattggtc ccataaatca catcccggac agcacattcc ataaatttca tactcacacg
     6841 tcctttaaat acagatgaca cttgccggaa ttatacagcc cagattcact ctcaatgttt
     6901 cagaatagct tataagccgc acttttaccg atactcaaca acaaatcagg tctggatcca
     6961 cttcagccgg aaaccggatc tgcgcggc
//
LOCUS       DADSZD010000199         6910 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.201, whole genome shotgun sequence.
ACCESSION   DADSZD010000199 DADSZD010000000
VERSION     DADSZD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.201"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            3..1769
                     /gene="adeD"
                     /locus_tag="J1Z28_003947"
     CDS             3..1769
                     /gene="adeD"
                     /locus_tag="J1Z28_003947"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBA7718726.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEFTE"
     gene            complement(1816..3207)
                     /gene="uhpT"
                     /locus_tag="J1Z28_003948"
     CDS             complement(1816..3207)
                     /gene="uhpT"
                     /locus_tag="J1Z28_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBA7718727.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3345..4664)
                     /gene="uhpC"
                     /locus_tag="J1Z28_003949"
     CDS             complement(3345..4664)
                     /gene="uhpC"
                     /locus_tag="J1Z28_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBA7718728.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSEMLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(4674..6176)
                     /gene="uhpB"
                     /locus_tag="J1Z28_003950"
     CDS             complement(4674..6176)
                     /gene="uhpB"
                     /locus_tag="J1Z28_003950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBA7718729.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(6176..6766)
                     /gene="uhpA"
                     /locus_tag="J1Z28_003951"
     CDS             complement(6176..6766)
                     /gene="uhpA"
                     /locus_tag="J1Z28_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBA7718730.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
BASE COUNT         1755 a         1991 c         1785 g         1379 t
ORIGIN      
        1 atatgaataa ttctattaac cataaatttc atcacattag ccgggctgaa taccaggaat
       61 tgttagccgt ttcccgtgga gacgctgttg ccgattatat tattgataat gtctctattc
      121 tcgacctgat caatggcgga gaaatttccg gcccaattgt gattaaagga cgttacattg
      181 ccggtgttgg cgcagaatac gctgatgctc cggctttgca gcggattgat gcccgcggcg
      241 caacggcggt gccagggttt attgatgctc acctgcatat tgaatccagc atgatgacgc
      301 cggtcacctt tgaaactgct accctgccgc gcggcctgac gaccgttatt tgcgaccctc
      361 atgaaatcgt caacgtgatg ggagaagccg gattcgcctg gtttgcccgc tgtgccgaac
      421 aggcaaggca aaaccagtac ttacaggtca gctcttgcgt acccgccctg gaaggctgcg
      481 atgttaatgg tgccagtttt acccttgaac agatgctcgc ctggcgggac catccgcagg
      541 ttaccggcct tgcagaaatg atggactacc ctggcgtaat tagcgggcag aatgcgctgc
      601 tcgataaact ggacgcattt cgccacctga cactggacgg tcactgcccg ggtttgggtg
      661 gtaaagaact taacgcctat attgctgcgg gtattgaaaa ctgccacgaa agttatcagt
      721 tggaagaagg acgccggaaa ttgcaactcg gcatgtcatt gatgatccgt gaagggtccg
      781 cagcccgcaa tctcaacgcg ctggcaccgt tgatcaacga atttaacagc ccgcaatgca
      841 tgctctgtac cgatgaccgt aacccgtggg agatcgccca tgaaggacac atcgatgcct
      901 taattcgccg cctgatcgaa caacacaatg taccgctgca tgtggcatat cgcgtagcca
      961 gctggtcgac ggcgcgccat tttggtctga atcacctcgg cttactggca cccggcaagc
     1021 aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac ggtgcagcag gtactggtga
     1081 aaggcgagcc gatcgatgca caaaccttac aggcggaaga gtcggcgaga ctggcacaat
     1141 ccgccccgcc atatggcaat accattgatc gccagccagt ttccgccagc gactttgccc
     1201 tgcaatttac ccccggaaaa cgctatcgcg ttattgaggt aatccataac gaactgatca
     1261 cgcactcccg ctccagcgtc tacagcgaaa acggttttga tcgcgatgat gtgtgcttta
     1321 ttgccgttct tgagcgttac ggacaacgac tggctccggc ctgtggtttg ctcggcggct
     1381 ttggcctgaa tgaaggtgcg ctggctgcga cggtcagcca tgacagccat aatattgtag
     1441 tgatcggtcg tagcgcagaa gagatggcgc tggcggtcaa tcaggtgatt caggatggcg
     1501 gcgggctgtg cgtggtccgt aacggccagg tacaaagtca tctaccgttg cccattgccg
     1561 ggctaatgag caccgacacg gcgcagtcac tggcggagca aattgacgcc ctgaaagccg
     1621 ccgcccgtga atgcggtccg ttacccgatg agccgtttat tcagatggcg tttctttccc
     1681 tgccagtgat ccccgcgctg aaactaacca gtcaggggct gtttgatggc gagaagtttg
     1741 ccttcactac gctggaattc acggaataat aaaaaaaagc ccggcgtcat gccgggcaaa
     1801 agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc ggcggatttt
     1861 gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg cggcgatatc
     1921 cagcgcggcg aaggtgcctg cccagccggt aaggccaaat accggcgtcc catcggcaat
     1981 cattcccaga cctaacttgg caaagctgtc accaatcagg taagcgaagg tgcctttaat
     2041 accatcggca gcgccaatcg cttttttagg tacaaagcca acagccgcca caccaatcaa
     2101 caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccaggt agatatattc
     2161 gttgctggcg tgttgataca caccgagcgt ggcgataatc agcgccagcg cgatacaggc
     2221 caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca gcgtaccgac
     2281 cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag agagtttcag
     2341 ttcctggaac gcatacacgg ttgaccactg gtcgatacca atacgcacca catagaggaa
     2401 aatgttggca aagcacagca gccagatcac tttgtttttc agcacatact caacaaagat
     2461 ctgccacttg gtcatatcgg tagattctgt ctctttatcc tcttcactga tctcctcgcc
     2521 aaacagctct tcagctttgc cgaggccata agattccggg gagtcgctgc cgtaacgcag
     2581 accaataaaa ccaacaatca gcgcgataat cgacgggaaa ataaacatgc cgatgacatg
     2641 accatcgaac aggtaatttg cgccgaacag cgccacacct gctgcgcctg caccaccaag
     2701 gttgtgagaa atgttccaga aaccgaggaa tgtcccgcgt ttacgacgcg gcgtccattt
     2761 ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc tcaaggcgta
     2821 gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac tgaagcccag
     2881 catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt ttttgccgtc
     2941 ggcgtagtaa gaaaccagcg ttttacccac gccataagtg atggagaagc ccaggccgat
     3001 catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct gcgcgatgtt
     3061 aaagttcttg cgaatcaggt acatcgtaag gtagccgata aagaccacca ggtaggattg
     3121 catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca gggtcggctt
     3181 gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc tgcgtgaggc
     3241 atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca aaacctggaa
     3301 aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc ttctcgcggt
     3361 gtctgggcgt tcaaaaaggg cagtaatagc agtgcggaaa tcccggcggc gatggcgata
     3421 accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg ccaaccagca
     3481 agcgacgcac caagataagc aaacaagccg acaaatcccg tcgccgcccc tgccgcctct
     3541 ttgtgggaac actctgccgc cgccatgccg attaacatct gcgggccaaa gacaaaaaaa
     3601 ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat cagccacagc
     3661 gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc gcggttgccg
     3721 ttaaacagtt tgtccgaacc ccaacccgct accagcgcac cgataaatcc gcccagttca
     3781 aacattgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcatctc ggacatatac
     3841 aaattgcccc agtcgttgat cgccgcccga accacataga ccagcacata gcaaaacgaa
     3901 agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt acgcgtcagc
     3961 cctgcccctt cttgttgttg agcaatctcc agtgcgtcgt gccgccattc accaaccgcc
     4021 ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat ccccacgact
     4081 atcgccatac aacccgcaat catcatcccg gcacgccagc cgtaatgcag cgcgctcgct
     4141 gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca taatgcccac
     4201 caaccgccgc gctcggtacg agaataccag gcggttaaca gacgcgcaca caccggtgaa
     4261 ccccagccct ggaaaaaggc gttcaacacc caaagcacgg caaacgccca tagcgacgtc
     4321 gagaagccaa acagaatgtt gataatgccc gtggcaataa gccctatccc cataaaataa
     4381 cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc gtaggtaatg
     4441 taaaacaggg tcgctaacag gccaatatcg ctacggctga gcacgccgtt agcaaggatt
     4501 tctggtacgg cggcgttaaa acttttacgc gtgaagtaaa acagcgcgta acccagccag
     4561 atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc atgtttatca
     4621 gtcattaatg gcgcatcggc gggcgctttc agaaacggca acatcatcga accttagaca
     4681 taacgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat ggtcaatgtg
     4741 ccacccagcg ccgttacgcg ctcgcgcatt ccggtaaggc caaaaccgtg ttgcccggaa
     4801 tctggcggta aaccgctgcc atcgtcttca atcaccagca tcaaccgttc atcctgctgc
     4861 cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt cagcccttcc
     4921 tggcagacac gaaacagcgt cactcgctgg ttttcgctta acgctgattc atcgattcgc
     4981 cattcgagat ggctgacaat gccgcgccct tccagctcca tttcccgcat cagtgagcgg
     5041 atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc taacaagcgg
     5101 cgcaccgcgt cgtaaacgcc caacgacaac tgttcgatga gctgcccgct ctgtttcacg
     5161 ctggcgttat ccgccgcgag ccgctggaca atgcccgctt gagttcgaat agcagtaatg
     5221 gtctgaccga tatcatcgtg cagctcacgt gccacatcac ggcgcacgct ctcttcggtt
     5281 tccagcaacc gttcagcgag atgctgattg cgcgccagtt ccttttgcag cgactggtta
     5341 agttcacgca accgctggat gccagcgccc agtaacaacc ctgtcagact ttgcaccagc
     5401 agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat cagcgcgatg
     5461 gcgttcatca atgtcgcaat cagcgctcct tgccaaccat agtgccaggc cagcgcgata
     5521 atcggcagcg ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa tcccaactgg
     5581 agccagagac tgataacaaa cagcagcaag taccagacca gatgccgccc acgccagttg
     5641 attggttgag aaaccagtga cgggccgagc ggcagccagg tgttattggc gagatagtgc
     5701 cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag cagtagcgca
     5761 ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc cgccgccgtt
     5821 aatgccgcac cctgtaacag caaggtgcgc cagtcacgtt gatggcgata gcgcgagatc
     5881 agcgccacgg gcagtaacgt cagtaaacta ccgatcatca ataaggagaa atgggttaaa
     5941 ccgaccgcct gcgttaacca gtaaatcagc agccactccg cgcccagcaa caccggccag
     6001 tatccgcgcg ggcattgcag cattagccct agccgcaggc caaacggaaa taacagtacc
     6061 gccatatcag ggcgctcaac caggtgcagg ctgatactcc acaggcaaaa ccatgcggca
     6121 gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt cttcatcacc
     6181 agccatcaaa catgcggcgc gccagttcaa cgtcgttact gacgcccagt ttttccatca
     6241 ggttggcgcg atggacatgt accgttttcg gtgacaagcc cagttcggca gcaatctctt
     6301 tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcgcgtttg gttagcgggt
     6361 cctggcgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag ccgcccgtgg
     6421 caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggag agaaaaccgc
     6481 gcgcccccgc gttaagcgcc tgctcaacca gcgccggact gtcatgaacg gagagcataa
     6541 ttgtcgccat gcctttcggc agctggctta gaagctccag accggagata tcaggcatgg
     6601 agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc gcctcgcgcc
     6661 ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc tgcgcaaagc
     6721 cggagcggac gataaggtga tcgtctataa gggcaacggt gatcatggtc ttgtcctggc
     6781 gggtaataaa acgcgcttac cttaacgata agcgcgccac tgttcaagcc ttgagcggtt
     6841 actgaaaaaa gctgcgctcc tgttgcttgt gaaaagaaat gatgccttca aggtttggta
     6901 tatcgactaa
//
LOCUS       DADSZD010000200         6902 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.202, whole genome shotgun sequence.
ACCESSION   DADSZD010000200 DADSZD010000000
VERSION     DADSZD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.202"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            456..1445
                     /locus_tag="J1Z28_003952"
     CDS             456..1445
                     /locus_tag="J1Z28_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7718731.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1515..3029
                     /locus_tag="J1Z28_003953"
     CDS             1515..3029
                     /locus_tag="J1Z28_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HBA7718732.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3041..4030
                     /locus_tag="J1Z28_003954"
     CDS             3041..4030
                     /locus_tag="J1Z28_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose ABC transporter permease AraH"
                     /protein_id="HBA7718733.1"
                     /translation="MMSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVP
                     NFATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTES
                     LWIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIED
                     ESFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPV
                     VRTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGK
                     ISYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4197..4997
                     /gene="otsB"
                     /locus_tag="J1Z28_003955"
     CDS             4197..4997
                     /gene="otsB"
                     /locus_tag="J1Z28_003955"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBA7718734.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4972..6396
                     /gene="otsA"
                     /locus_tag="J1Z28_003956"
     CDS             4972..6396
                     /gene="otsA"
                     /locus_tag="J1Z28_003956"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBA7718735.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6403..6831)
                     /gene="uspC"
                     /locus_tag="J1Z28_003957"
     CDS             complement(6403..6831)
                     /gene="uspC"
                     /locus_tag="J1Z28_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBA7718736.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
BASE COUNT         1731 a         1620 c         1806 g         1745 t
ORIGIN      
        1 tacgtgttct gttaattaat gagtacagtg ataattaatg gcgaaatgaa tgacgcgcat
       61 tttccacatc tttaagttgc ggttattaca ccattgcaaa aactcaacgc caggtaatga
      121 ggcctattga ctggttaaaa agaaaacatc ccgcatgggt accaaagaca acaaggactt
      181 ccaggctaat cttatggatt attctgctgt gcactcgata atttgtctga caaattcgct
      241 tttccttatg tcttttcccg ctaaatttat gcacgttctc actgtaattc tgcgatgtga
      301 tattgctctc ctatggagaa ttaatttctc gctaaaacta tgtcaacaca gtcacttatc
      361 ttttagttaa aaggtaatgc tttgttttcc gattaattta acgaatgtca ttcgtttttg
      421 ccctacacaa aacgacacta aagctggaga gaaccatgca caaatttact aaagccctgg
      481 cagccattgg tctggcagcc gttatgtcac aatccgctat ggcggagaac ctgaagctcg
      541 gtttcctggt gaagcaaccg gaagagccgt ggttccagac cgaatggaag tttgccgata
      601 aagccgggaa ggatttaggg tttgaagtta ttaagattgc cgtaccggat ggcgaaaaaa
      661 cattgaacgc tatcgacagc ctggctgcca gtggcgcgaa aggtttcgtt atttgtactc
      721 cggacccgaa actcggctct gccatcgtgg cgaaagcgcg tggctacgat atgaaagtca
      781 ttgccgtgga tgaccagttt gttaacgcca aaggtaagcc aatggatacc gttccgctgg
      841 tgatgatggc tgcgaccaaa attggcgaac gtcagggcca ggaactgtat aaagagatgc
      901 agaaacgtgg ctgggatgtc aaagaaagcg cggtgatggc gattacctcc aacgaactgg
      961 atacggctcg tcgccgtact acggggtcta tggatgcgct gaaagctgca ggattcccgg
     1021 aaaaacaaat ttatcaggta cctaccaaat ctaacgacat cccgggggca tttgacgctg
     1081 ccaactcaat gctggttcaa catccggaag ttaaacattg gctgattgtc ggtatgaacg
     1141 acagcaccgt gctgggcggc gtacgcgcga cggaaggtca gggctttaaa gcgagcgata
     1201 tcatcggtat cggtattaac ggtgtggatg cggtgagtga actgtctaag gcacaggcaa
     1261 ccggtttcta cggttccctg ctgccaagcc cggacgtaca tggttataag tccagcgaaa
     1321 tgctttacaa ctgggttgca aaaggtgttg aaccgaccaa attcaccgaa gttaccgacg
     1381 tggtgctgat cacgcgtgac aactttaaag aagaactgga gaaaaaaggt ttaggcggta
     1441 agtaatttgc cggaaaaatt cccctctgca tgatgcagag ggggtgtgaa cgaccagtga
     1501 ttcacggaga cgttatgcaa cagtctaccc cgtatctctc atttcgcggc atcggtaaaa
     1561 cgtttcccgg cgttaaggcg ctgacggata ttagttttga ctgctatgcc ggtcaggttc
     1621 atgcgttgat gggtgaaaat ggcgcaggaa aatcaactct cttaaaaatc ctcagcggaa
     1681 actatgcgcc aaccacgggt tctgtagtga ttaatgggca ggaaatgtcc ttttccgaca
     1741 cgaccgcagc acttaacgcg ggggtggcga ttatttacca ggaactgcat ctcgtgccgg
     1801 aaatgaccgt cgcggaaaac atctatctcg gccagctgcc gcataaaggc ggcattgtga
     1861 atcgttcatt gctgaattat gaggcaggtt tacaaattaa acatcttggt atggatattg
     1921 acccggacac gccgctgaaa tatctctcca ttggtcagtg gcagatggtt gaaatcgcca
     1981 aagcgctggc gcgtaacgcc aaaattatcg cctttgatga gccaaccagc tccctctctg
     2041 cccgcgaaat cgacaatctt ttccgcgtta ttcgtgaact gcgaaaagag gggcgggtga
     2101 tcttatacgt ttctcaccgt atggaagaaa tatttgccct cagcgatgcc atcaccgtct
     2161 ttaaagatgg gcgttatgtc aaaaccttta ccgatatgca gcaggttgac cacgacgcac
     2221 tggtgcaggc gatggtcggg cgagacattg gcgatatcta cggctggcaa ccgcgtagtt
     2281 atggcgagga gcggctgcgt cttgatgctg tgaaagcacc aggcgtgcgt acgccaataa
     2341 gtctggcggt tcgcagtgga gaaattgtcg gtctgtttgg tctggtcggg gcagggcgta
     2401 gcgaattaat gaaaggcatg tttggcggga cgcaaatcac cgccggtcag gtttatatcg
     2461 accaacagcc gatcgatatt cgtaaaccga gccacgccat tgccgcaggc atgatgctct
     2521 gcccggaaga tcgcaaagcg gaaggcatta ttcccgtgca ctccgttcgc gacaatatca
     2581 acatcagtgc cagacgtaaa catgtgcttg gaggttgtgt aatcaacaac ggttgggaag
     2641 aaaacaatgc cgatcaccac attcgttcgc tcaacatcaa aacgccgggc gctgagcaac
     2701 tgattatgaa tctctcaggt ggaaatcagc aaaaagccat tctgggccgc tggttatcgg
     2761 aagagatgaa ggtcattttg ctggatgaac ctacgcgcgg cattgatgtt ggtgctaagc
     2821 acgaaatata taacgtgatt tatgcgctgg cggcgcaggg ggtggcggtg ctgtttgcct
     2881 ccagcgactt acctgaagtc ctcggcgttg ccgaccggat tgtggtgatg cgcgaaggtg
     2941 aaatcgccgg tgaattgtta cacgagcagg cagatgagcg tcaggcactg agccttgcga
     3001 tgcctaaagt cagccaggca gttgcctgag taaggagagt atgatgtctt ctgtttctac
     3061 atcgggttct ggcgcaccta agtcgtcatt cagcttcggg cgtatctggg atcagtacgg
     3121 catgctggtg gtgtttgcgg tgctttttat cgcctgtgcc atttttgtcc caaattttgc
     3181 caccttcatt aatatgaaag ggttgggcct ggcaatttcc atgtcgggga tggtggcttg
     3241 tggcatgttg ttctgccttg cttccggtga ctttgacctt tctgtcgcct ccgtaattgc
     3301 ctgtgcgggt gtcaccacgg cggtggttat taatctgact gaaagcctgt ggattggcgt
     3361 ggcagcgggg ttgctgctgg gcattctctg tggtctggtc aatggctttg ttatcgccaa
     3421 actcaaaata aatgccctga tcacgacact ggcaacgatg cagattgttc gaggtctggc
     3481 gtacatcatt tcagacggta aagcggtcgg tatcgaagat gaaagcttct ttgcccttgg
     3541 ttacgccaac tggttcggtc tgcctgcgcc aatctggctc accgtcgcgt gtctgatcat
     3601 ctttggcttg ctgctgaata aaaccacctt tggtcgtaat accctggcga ttggcgggaa
     3661 cgaagaggct gcgcgtctgg cgggtgtacc ggttgttcgc accaaaatta ttatctttgt
     3721 tctctctggc ctggtatcag cgatagccgg aattattctg gcttcacgta tgaccagtgg
     3781 gcagccaatg acgtcgattg gctatgagct gattgtgatc tccgcctgcg ttttaggtgg
     3841 cgtttctctg aaaggcggca tcggaaaaat ctcatatgtg gtggcgggta tcttaatttt
     3901 aggcaccgtg gaaaacgcca tgaacctgct taatatttct cctttcgcac agtacgtagt
     3961 tcgcggctta atcctgctgg cagcggtgat tttcgaccgt tacaagcaaa aagcgaaacg
     4021 cactgtctga tgcttttttc cacaacaatt tagcgttttt tcccaccaca gccagccgcc
     4081 acaacggttg gctgttcttc gttgcaaatg gcgacccccg tcacactgtc tatacttaca
     4141 tgtctgtaaa gcgcgttctg cgcaacacaa taagaaaaga gaaggaggag aaccgggtga
     4201 cagaaccgtt aaccgaaacc cctgaactat ccgcgaaata tgcctggttt tttgatctcg
     4261 atggaacgct ggcggaaatc aaaccgcatc ccgatcaggt cgtcgtacct gacactattc
     4321 tgcaaggact tcagctactg gcaaccgcaa gtgatggtgc attggcattg atatcagggc
     4381 gctcaatggt ggagcttgat gcactggcaa aaccttatcg cttcccatta gcgggcgtgc
     4441 atggggcgga gcgccgtgac atcaatggta aaacgcatat cgttcatctg ccggatgcga
     4501 ttgcgcgtga tattagcgtg caactgcata cggtcatcgc ccagtatccc ggcgcggagc
     4561 tggaggctaa agggatggct tttgcgctgc attatcgtca ggctccgcag catgaagacg
     4621 cattaatgac attagcgcaa cgtattactc agatctggcc acaaatggcg ttacagcagg
     4681 gaaagtgtgt tgtcgagatc aaaccgaaag gtaccagtaa aggtgaggcg attgctgcct
     4741 ttatgcagga agctcccttt atcgggcgaa cgcccgtatt tctgggcgat gatctgaccg
     4801 atgaatctgg cttcgcagtc gttaaccggc tgggcggaat gtcagtaaaa attggcacag
     4861 gtgcaactca ggcatcatgg cgactggcgg gcgtgccgga tgtctggagc tggcttgaaa
     4921 tgataaccac cgcattacaa caaaaaagag aaaataacag gagtgatgac tatgagtcgt
     4981 ttagtcgtag tatctaaccg gattgcacca ccagacgagc acgccgccag tgccggtggc
     5041 cttgccgttg gcatactggg ggcactgaaa gccgcaggtg gactgtggtt tggctggagc
     5101 ggtgaaacag ggaatgagga tcagccgcta aaaaaggtga aaaagggtaa cattacgtgg
     5161 gcctctttta acctcagcga acaggacctt gacgaatact acaacaaatt ctccaatgcc
     5221 gttctctggc ccgcttttca ttatcggctc gatctggtgc aatttcagcg tcctgcctgg
     5281 gatggctatc tacgcgtaaa tgcgttgctg gcagataaat tactgccgct gttgcaagac
     5341 gatgacatta tctggatcca cgattatcac ctgttgccct ttgcgcatga attacgcaaa
     5401 cggggcgtga ataatcgcat tggtttcttt ctgcatattc ctttcccgac accggaaatc
     5461 ttcaacgcgc tgccgacata tgacaccttg cttgaacagc tttgtgatta tgatttgctg
     5521 ggtttccaga cggagaacga tcgtctggcg ttcctggatt gcctttctaa cctgacccgc
     5581 gtcacgacac gtagcgcaaa aagccatacg gcctgcggca aagcgtttcg aacggaagtt
     5641 tacccgatcg gcattgagcc gaaagaaata gcgaaacagg ctgccggtcc actgccgcca
     5701 aaactggcgc aactcaaagc agaactgaaa aacgtacaaa atatcttttc tgtcgaacga
     5761 ctggattatt ccaaaggttt gccggagcgt tttctcgcct atgaagcgtt actggaaaaa
     5821 tatccgcaac atcacggtaa aattcgttat acccagatcg cgccaacgtc tcgtggtgat
     5881 gtgcaagcct atcaggatat tcgtcatcag ctcgaaaatg aagccggacg aattaatggt
     5941 aaatatgggc aattaggctg gacgccgctt tattatttga atcagcattt tgaccgtaaa
     6001 ttactgatga aaatattccg ctactctgac gtgggcttag tgacgccact gcgtgacgga
     6061 atgaacctgg tggcaaaaga gtatgttgcc gctcaggacc cagccaaccc gggcgttctt
     6121 gttctttcgc aatttgcggg agcggcaaac gagttaacgt cggcgttaat tgttaatccc
     6181 tacgatcgtg acgaagttgc agctgcgctg gatcgtgcat tgactatgtc gctggcggaa
     6241 cgtatttccc gtcatgcaga aatgctggac gttatcgtga aaaacgatat taaccactgg
     6301 caggagtgct tcattagcga cctaaagcag atagttccgc gaagcgcgga aagccagcag
     6361 cgcgataaag ttgctacctt tcctaagctt gcgtaggtgc ggttaatctc ccgtaagtgg
     6421 aactaacagc acatcgacct cacttgaggt aatcactctt ttcgccgagc aggatgctcg
     6481 cgaaaagaaa ctgtgattgt gattaccgca aatcaccaaa tcgaaatgat gtttgcgaca
     6541 aacttccaga atatgttcgc ttaactcacc gtaggcaata aacgtcttgt caacgggata
     6601 ccccgcatcc tgaattaact tatcaagaaa gctttgcgtt tcttcctgca tcacattgcg
     6661 taaatcttca agcatcggcg cagctaattg attgtacatt tccgggtcgg aagcgagagt
     6721 aattaaactg atgtgtccct ttactggcct ggcgatagat accgcttttg ccagcagttg
     6781 ctgactttcc ggtgtaaccg caacggcgac aagaatattg ctatagctca taacctgttc
     6841 cttattctgt gaacttcagg tgacattaaa gctttactcc taatggagaa acgacgcaat
     6901 cc
//
LOCUS       DADSZD010000201         6892 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.203, whole genome shotgun sequence.
ACCESSION   DADSZD010000201 DADSZD010000000
VERSION     DADSZD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.203"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            99..1496
                     /gene="ulaA"
                     /locus_tag="J1Z28_003958"
     CDS             99..1496
                     /gene="ulaA"
                     /locus_tag="J1Z28_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBA7718737.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            1512..1817
                     /gene="ulaB"
                     /locus_tag="J1Z28_003959"
     CDS             1512..1817
                     /gene="ulaB"
                     /locus_tag="J1Z28_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBA7718738.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            1827..2291
                     /gene="ulaC"
                     /locus_tag="J1Z28_003960"
     CDS             1827..2291
                     /gene="ulaC"
                     /locus_tag="J1Z28_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBA7718739.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            2305..2955
                     /gene="ulaD"
                     /locus_tag="J1Z28_003961"
     CDS             2305..2955
                     /gene="ulaD"
                     /locus_tag="J1Z28_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBA7718740.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            2965..3819
                     /locus_tag="J1Z28_003962"
     CDS             2965..3819
                     /locus_tag="J1Z28_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBA7718741.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            3819..4505
                     /locus_tag="J1Z28_003963"
     CDS             3819..4505
                     /locus_tag="J1Z28_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBA7718742.1"
                     /translation="MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4634..4909)
                     /locus_tag="J1Z28_003964"
     CDS             complement(4634..4909)
                     /locus_tag="J1Z28_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7718743.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            5237..5632
                     /gene="rpsF"
                     /locus_tag="J1Z28_003965"
     CDS             5237..5632
                     /gene="rpsF"
                     /locus_tag="J1Z28_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBA7718744.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            5639..5953
                     /gene="priB"
                     /locus_tag="J1Z28_003966"
     CDS             5639..5953
                     /gene="priB"
                     /locus_tag="J1Z28_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBA7718745.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            5958..6185
                     /gene="rpsR"
                     /locus_tag="J1Z28_003967"
     CDS             5958..6185
                     /gene="rpsR"
                     /locus_tag="J1Z28_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBA7718746.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            6227..6676
                     /gene="rplI"
                     /locus_tag="J1Z28_003968"
     CDS             6227..6676
                     /gene="rplI"
                     /locus_tag="J1Z28_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBA7718747.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         1647 a         1712 c         1894 g         1639 t
ORIGIN      
        1 ataaataatc ttgttgtgat tacttttgaa aattagagtg agagcacaac attccgggtg
       61 tgcgaaatac ccggttacct cttcttcagg agatcgttat ggagatcctc tacaacatct
      121 ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt attgtgacct
      181 gtctgggtta catcctactg cgcaaaagtg tcagcgttat tattaaaggt acgattaaaa
      241 ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctgaccagc accttcaaac
      301 cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc gatacttacg
      361 cgtcgatgat ggcaaccatt gaacgcatgg gcgatgccta tagctgggtg ggttacgcgg
      421 tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt accggtattc
      481 gtaccatcat gctgactggt cacatcatgt tccagcaggc ggggctgatt gccgttacgc
      541 tgtttatctt cggctactcc atgtggacga ccattatctg caccgcgatt ctggtttcgc
      601 tctactgggg cattacctcc aacatgatgt acaagccgac acaggaagtc accgacggct
      661 gtggtttctc cattggtcac cagcagcagt tcgcatcatg gattgcttat aaagtcgcac
      721 cgttcctcgg caaaaaagag gagagcgttg aagacctcaa actgcctggc tggctgaata
      781 ttttccacga caacatcgtc tccacggcga ttgtcatgac tatcttcttt ggtgccattc
      841 tgctctcctt tggtatcgac accgtgcagg cgatggcagg caaagtgaac tggacggtgt
      901 acatcctgca aaccggtttc tccttcgcgg tggcgatctt catcatcact cagggcgtgc
      961 gcatgtttgt ggcggagctc tctgaagcat tcaacggtat ctcccagcgc ctgatcccgg
     1021 gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgcgccgaac gccgtggtct
     1081 ggggttttat gtggggcacc atcgggcaac tgattgcggt tggcatcctg gtcgcctgtg
     1141 gctcttcaat cctgattatt ccaggcttta tcccgatgtt cttctccaac gccactatcg
     1201 gcgtgttcgc taaccacttc ggcggctggc gcgcggcgct gaagatttgc ctggtgatgg
     1261 ggatgatcga aatctttggt tgcgtctggg cggtgaaact caccggtatg agcgcctgga
     1321 tgggcatggc ggactggtcg attctggcac cgccgatgat gcagggcttc ttctccatcg
     1381 gtatcgcctt tatggccgtc atcattgtca ttgcactggc ttatatgttc ttcgctggcc
     1441 gcgcactgcg tgcagaagaa gatgcagaaa aacaactggc agaacaatct gcttaataag
     1501 gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg taacggacaa ggcagttcca
     1561 tgatcatgaa aatgaaagtg gaccagtttt taacccagtc gaacatcgac cacacggtaa
     1621 acagctgcgc ggttggcgaa tacaaaagcg agttgagcgg tgcggatatc atcatcgctt
     1681 ctacccatat tgcgggcgaa atcaccgtga ccggcaacaa atatgtggtt ggcgtgcgca
     1741 acatgctctc tcctgctgat tttggcccga aactgctgga agtgatcaaa gagcatttcc
     1801 cgcaggacgt gaagtaagga cgcgccatga aattacgtga ttcgctggct gaaaataaat
     1861 ctatccgcct gcaagcggaa gcagagacat ggcaggacgc agtgaaaatc ggcgttgatc
     1921 tgctggtagc ggcagatgtg gtagagccgc gttactacca ggcgattctg gatgcggttg
     1981 aacagcacgg cccgtacttt gtgctcgcac ccggcctggc aatgccgcac ggacgcccgg
     2041 aagagggggt taagaaaact ggttttgcgc tggtgacgct gaaaaagccg ctggagttca
     2101 accacgaaga taacgatccg gtggatatcc tcatcaccat ggcggcggtt gatgccaaca
     2161 ctcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa gagaatttcg
     2221 accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac cgcaccaacg
     2281 cggcagctta agaaggaatt gaacatgtca ttaccgatgt tgcaagtcgc gctggacaac
     2341 cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt cgacattatc
     2401 gaagtgggca ccattctttg cgtaggcgaa ggcgtgcgtg cggttcgtga cctgaaagcg
     2461 ctgtacccgc acaaaatcgt actggctgac gccaaaattg ccgatgcagg caaaatcctt
     2521 tctcgcatgt gcttcgaagc caacgctgac tgggtgaccg taatttgctg tgcggatatc
     2581 aacaccgcca aaggcgcgct ggacgtggca aaagagttta atggcgacgt gcagatcgaa
     2641 ttgaccggct actggacctg ggaacaggcg cagcagtggc gcgacgcagg cattgggcag
     2701 gtggtttatc accgcagccg tgacgcgcag gccgcaggcg tggcatgggg cgaagcggac
     2761 attaccgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac cggtggtctg
     2821 gcgctggaag atctgccgtt gtttaaaggc attccgattc atgtgtttat cgctggtcgc
     2881 agcattcgtg atgccgcttc tccggtggag gccgcacgtc agttcaaacg ttccatcgct
     2941 gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg catctacgaa
     3001 aaagcgctcc ccgctgggga gtgctggctg gaacgactgc aactggcaaa aacgttaggc
     3061 ttcgattttg tcgagatgtc agtagatgaa acggacgatc gcctttcgcg cctcgactgg
     3121 agccgcgagc agcgtctggc actggtgaat gcgattgttg aaaccggcgt gcgcgtaccg
     3181 tccatgtgcc tttctgctca tcgtcgtttc ccgctgggca gcgaagatga cgcggtgcgg
     3241 gcccaggggc tggagattat gcgtaaagct atccagttcg cccaagacgt cggtattcgc
     3301 gtgatccaac tggcgggcta tgacgtttac tatcaggaag ccaataacga aacgcgtcgt
     3361 cgtttccgtg acggtctgaa agagagcgtt gagatggcga gccgcgcgca ggtcacactg
     3421 gcgatggaga tcatggatta cccattgatg aactccatca gcaaggcgct gggctacgca
     3481 cactatctca acaatccgtg gttccagctc tacccggata tcggcaacct gtcggcgtgg
     3541 gacaacgacg tgcagatgga gttgcaggcc ggaattgggc atatcgtcgc ggtacatgtg
     3601 aaagacacca aaccaggcgt cttcaaaaac gtgccgtttg gcgaaggcgt agtggatttc
     3661 gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct gattgagatg
     3721 tggagcgaaa cggcggaaga cccggcggca gaagtagcga aagcgcgtga ttgggtgaaa
     3781 gcgcgcatgg ccaaagcagg catggtggag gcggcataat gcaaaagctg aaacagcagg
     3841 tgtttgaagc caacatggat ttaccgcatt acgggttggt gacctttacc tggggcaacg
     3901 tcagcgctat tgaccgtgaa cgcgggctgg tggtgatcaa gcccagcggt gtcgcctacg
     3961 aaaccatgaa agcggacgat atggtggtgg tcgatatgag cggtaaggtg gtggaagggg
     4021 agtatcgtcc gtcttccgac actgcgacgc atctcgaact ctaccgtcgt tatccgtcgc
     4081 ttggtggtat tgtccatacc cactccactc atgccaccgc atgggcgcag gcggggctgg
     4141 cgatcccggc gttaggcacc acgcacgccg attacttctt tggcgacatc ccgtgcacgc
     4201 gcggattaag cgaagaagag gtgcagggtg agtacgaact gaataccggc aaagtgatta
     4261 tcgaaacgct gggtgacgcc gagccgctgc atacgccggg aattgtggtg tatcagcacg
     4321 gaccgttcgc ctgggggaaa gatgcccacg acgcggtgca taacgcggtg gtgatggaag
     4381 aagtggcgaa aatggcgtgg attgcgcgca gtattaatcc acaactgaat cacatcgaca
     4441 gcttcctgat gaataaacac ttcatgcgta agcacggtcc caacgcctat tacgggcaga
     4501 agtaaaacac gcgctgcgga aatttccttc ttcgggagat aactggtcta attccgcagc
     4561 cgtttttcaa aaaaagcccc ctgcgaaggg ggcaaagcaa actatggcaa tgtttcgttg
     4621 gttatacctg gtgctagcga taaatatccg cgctggcgtg catattgccg ttactccccg
     4681 gttcccgcat cagaatcaca tggtagtacg ttgcgccttg cgcatctgtc tcttcattta
     4741 atgcctgacg tgcttcactt tcagtggcga agttatggtt gatataaatc acgcctaagc
     4801 tttgcacatc gtccatattt ctggcctggt ggttattaat ttcaatggct gcccatgtat
     4861 ttgcacttag caaaagcaca gccagaaggg ctaaaacgcg actgaacata gatacctcct
     4921 cgacggctga ctttgtgtgc tctcctcttg tggtgatctt ctgatttaat tttaatcaat
     4981 gataaagaag ttgatggtga ccatttctga tgcagttgtt caaaaaaaca ccatgacgaa
     5041 gtgtgatgaa ctccaaatca gcggtttaga ggttatttgc gaaaagggag atttatttcg
     5101 gctctgccct tgagtttagc ggggtatgtc agtactataa cgcgtcattt ttcagccgac
     5161 ctttaacacg ttccttgcct ccccgggatt cggctgaccc agacaggagg ctgaataatc
     5221 cgtaaggagc aattcgatgc gtcattacga aatcgttttt atggtccatc ctgaccagag
     5281 cgaacaggtt ccgggcatga tcgagcgcta cactgctgcc atcactggtg cagaaggcaa
     5341 gatccaccgt ctggaagact ggggccgccg tcagctggct tacccgatca acaaactgca
     5401 caaagcacac tacgttctga tgaacgttga agctccgcag gaagtgatcg atgagctgga
     5461 aactaccttc cgcttcaacg atgccgttat ccgcagcatg gttatgcgta ccaagcacgc
     5521 tgttaccgaa gcatctccga tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga
     5581 tttcgcaaac gaaaccgctg atgatgctga agctggggat tctgaagagt aatttctgat
     5641 gaccaaccgt ctggtgttgt ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc
     5701 atcaggaatt cctcactgcc agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg
     5761 ctttcaccgg caggcatggt gccaaatgcc cgttattgtt agcggacacg aaaaccaggc
     5821 cattactcac agtataacgg tcggcagtcg cataaccgtt caggggttca tttcatgcca
     5881 taaggcaaag aacggactga gcaaaatggt tttgcatgcc gagcagattg aattgataga
     5941 ttctggagac tagccatatg gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg
     6001 cggaaggcgt tcaagagatc gactataaag atatcgctac gctgaaaaac tacatcaccg
     6061 aaagcggtaa gattgtccca agccgtatca ccggtacccg tgcaaaatac cagcgtcagc
     6121 tggctcgcgc tatcaaacgc gctcgctacc tgtccctgct gccgtacact gatcgccatc
     6181 agtaatcggt cacggtccat taatacgact ttgagaggat aaggtaatgc aagttattct
     6241 gcttgataaa gtagcaaacc tgggtagcct gggtgatcag gtaaacgtta aagcgggcta
     6301 tgctcgtaac ttcctggtac cgcagggtaa agctgttcca gctaccaaga aaaacattga
     6361 attcttcgaa gcacgtcgcg ctgaactgga agctaaactg gctgaagttc tggcagcagc
     6421 taatgctcgc gctgagaaaa tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg
     6481 cgacgaaggt aaactgttcg gttccatcgg tactcgcgac atcgctgacg ctgtaactgc
     6541 agctggcgtt gaagtggcta agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac
     6601 tggcgaacac gaagtgagct tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa
     6661 cgtagtagct gaataattcg ttattcaacg agacgtaaaa acgccgacca gtggtcggcg
     6721 ttttgctttt aagtgataaa aaccgcagaa acagacgttc cctccataaa tctcttgtta
     6781 ttccttttat aaaaagttcg cagtacccat acctggcccc cagcctcgcc gctggtagtt
     6841 cgtgtcatgt tttttacgcg taaaacacga caaaagcatc aatacagcga tc
//
LOCUS       DADSZD010000202         6818 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.204, whole genome shotgun sequence.
ACCESSION   DADSZD010000202 DADSZD010000000
VERSION     DADSZD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6818
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.204"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            41..538
                     /gene="ftnA"
                     /locus_tag="J1Z28_003969"
     CDS             41..538
                     /gene="ftnA"
                     /locus_tag="J1Z28_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBA7718748.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(576..815)
                     /locus_tag="J1Z28_003970"
     CDS             complement(576..815)
                     /locus_tag="J1Z28_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBA7718749.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1006..2217
                     /gene="tyrP"
                     /locus_tag="J1Z28_003971"
     CDS             1006..2217
                     /gene="tyrP"
                     /locus_tag="J1Z28_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBA7718750.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKQRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2279..2944)
                     /gene="yecA"
                     /locus_tag="J1Z28_003972"
     CDS             complement(2279..2944)
                     /gene="yecA"
                     /locus_tag="J1Z28_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBA7718751.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3140..3226)
                     /locus_tag="J1Z28_003973"
     tRNA            complement(3140..3226)
                     /locus_tag="J1Z28_003973"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3190..3192),aa:Leu,seq:taa)
     gene            complement(3239..3312)
                     /locus_tag="J1Z28_003974"
     tRNA            complement(3239..3312)
                     /locus_tag="J1Z28_003974"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3278..3280),aa:Cys,seq:gca)
     gene            complement(3366..3441)
                     /locus_tag="J1Z28_003975"
     tRNA            complement(3366..3441)
                     /locus_tag="J1Z28_003975"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3406..3408),aa:Gly,seq:gcc)
     gene            complement(3593..4141)
                     /gene="pgsA"
                     /locus_tag="J1Z28_003976"
     CDS             complement(3593..4141)
                     /gene="pgsA"
                     /locus_tag="J1Z28_003976"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBA7718752.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4198..6030)
                     /gene="uvrC"
                     /locus_tag="J1Z28_003977"
     CDS             complement(4198..6030)
                     /gene="uvrC"
                     /locus_tag="J1Z28_003977"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBA7718753.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGA
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6027..6683)
                     /gene="uvrY"
                     /locus_tag="J1Z28_003978"
     CDS             complement(6027..6683)
                     /gene="uvrY"
                     /locus_tag="J1Z28_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBA7718754.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1642 a         1778 c         1616 g         1782 t
ORIGIN      
        1 gacgccacag caacaaatat aacctttgtg gagcactatc atgctgaaac cagaaatgat
       61 tgaaaaactt aatgagcaga tgaacctgga actgtactct tcactgcttt atcagcaaat
      121 gagcgcctgg tgcagctatc ataccttcga aggtgctgcc gcgttcctgc gccgtcacgc
      181 ccaggaagag atgacgcata tgcagcgtct gtttgattac ctgactgata ccggcaactt
      241 accgcgtatt aataccgttg aatctccgtt tgctgaatat tcctcacttg atgaattatt
      301 ccaggaaacc tataaacacg aacaattaat cacgcagaaa attaacgaac tggctcatgc
      361 tgcaatgacc aatcaggact acccaacatt taatttcctg caatggtatg tttctgagca
      421 gcatgaagaa gagaaactgt tcaaatcgat tattgataaa ttaagcctgg caggcaaaag
      481 tggcgaaggt ctgtatttta tcgacaaaga actctctacc ctcgacacac aaaactaatg
      541 ctctccggcg gcaggttttc tgccgccgtt tcaaatcagt gacggcaaat cttactctga
      601 tcggttgaaa aaccttcttc tgaaggtata aatttgcctt ttgctgccag aaacgccacc
      661 aactctcccg cagtcatccc ttctgccgag caggtgtgaa aacgtgcctg ttcaccaaaa
      721 cgcgctttaa ttgctgcttc cagactggca tgcgtatatt gctcgcctga ttcaatcatc
      781 atatttaaca cttcgtgacc gtgaatagag tccatcgttc ctcctcaaaa aaagcctagc
      841 gtagcgattg ccgcttatga agactttgcg ccagcgcaga actgaacgct ttttattgta
      901 catttatatt tacaacatat gtaacgtcgg tttgacgaat cagccgttat gccttaacct
      961 gcgccgcaga tatcactcat aaagatcgtc aggacagaag aaagcgtgaa aaacagaact
     1021 ctgggaagtg tttttatcgt ggcgggaacc acaattggcg ctggtatgct ggcaatgccg
     1081 ctggctgcgg caggtgttgg ttttagcgtc acgttaatct tgttaattgg gctttgggcg
     1141 ttgatgtgct acacggcgct attactgctg gaggtgtacc agcatgtccc ggcagatacc
     1201 ggtctgggca cgctggcaaa acgctatctg ggacgctacg gtcagtggct gacgggcttc
     1261 agtatgatgt tcttaatgta tgctctgact gcggcataca tcagcggtgc cggtgaattg
     1321 ttggcctcca gcatcagcga ctggacaggt atttcaatgt cggcaaccgc tggcgtgctg
     1381 ttgttcactt ttgttgccgg tggcgtggtt tgtgtcggaa cttcgctggt cgatttattt
     1441 aaccgttttc tgttcagcgc caaaattatt tttttggtgg tgatgctggt attgctgcta
     1501 ccgcatattc acaaagtgaa tcttttaacc ctgccgttgc aacagggact ggcactgtct
     1561 gcaatcccgg tgatttttac gtcgtttggt tttcacggta gcgtgccgag tattgtcagc
     1621 tatatggatg gcaacattcg taagcaacgc tgggtgttta taatcggtag tgcgatcccc
     1681 ctggtggcat atattttctg gcagctggcg acccttggca gcattgattc aacaaccttt
     1741 atgggattgc tggctaatca tgccggatta aacgggctgt tacaggcgtt acgcgaaatg
     1801 gtggcctctc cgcatgttga gctggcagtg catttatttg ctgatttagc cctcgccacg
     1861 tccttcctcg gcgtagcgtt aggcttattt gattatctgg cagatttgtt tcagcgttca
     1921 aataccgttg gtggacgatt gcaaactggt gcaattacct ttctgccgcc actggcgttt
     1981 gcactgtttt atccacgagg atttgtgatg gcgctgggtt acgccggtgt ggcactggcg
     2041 gtactggcat tgattatccc ttcgctgttg acctggcaaa gcaggaagca caatcctcag
     2101 gcgggttacc gggtgaaagg tggtcgtccg gcgctggtgg tggtgtttct ctgtggtatt
     2161 gctgtgattg gcgtgcaatt tttgattgcg gcaggcttgt taccagaagt ggggtgatta
     2221 gatagcctca aattccttat tgggtgccag aattaacgct gacacccaat gtgacctctt
     2281 aatgcaggca gcactgctta aatttcttac cactaccgca cggacaagga tcgttacgcc
     2341 ccggtttctc ttctgctttg ataggttgct gaacagcttt ttcctgcgga tgcgccatcc
     2401 agtatgcatg taaatcaagc gccgccagtc gaatagcatc tacgctctct tcaaacgctt
     2461 ctggcgacat cttctctacc cgctcgaagt tttcctcagt accgtgcagc gcaatcgcct
     2521 ccagcgctgg ttttaacgaa tcgggcaacg ttgaccagtc agaaagtgcc acgccccgca
     2581 tatagccaaa gcaccactcc tcaacaatcg tcagctcgct gccatcgact tctcgcaagc
     2641 cgaataacgg ctcaaactgc tccgggaatt cgttcagacg ctctgcggta tcggccatat
     2701 gttgaaaagc caggttcata aagcgcgtca tctctttctc tgacgcccag cgcggcacat
     2761 agtcagcccc accccacacg gcaaccagcc actgtgccgg ttcaatctct tgcggagaac
     2821 tcaacaccgc cgtcaataaa ccgtccagct ccgccacatc aagaatggcg tggtcagtgt
     2881 tgtatttggt cagaatatcg tccagccatt ccaactcact ttcgtttaac ggtcccgttt
     2941 tcatacgctt ttccttgtgg atctcaactc gccagcacct atcttacatg ccggtccgta
     3001 tcagagatac tttttgagtg gctttgccgg tgattaaaaa ttaaggaggg tgtaacgaca
     3061 agttgcaggc acaaaaaaac cacccgaagg tggtttcacg acactgctta ttgctttgat
     3121 tttattctta tctttcccat ggtacccgga gcgggacttg aacccgcaca gcgcgaacgc
     3181 cgagggattt taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg
     3241 gaggcgcgtt ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt
     3301 tgttaacgcg ccaaattctt caggcctttc agccagacat ccgctgacgc cgatgtcttt
     3361 taaactggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
     3421 taccaactga gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc
     3481 gtctggcaat cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc
     3541 aacgatattt ttcaccactt ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat
     3601 caagcaaatc tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg
     3661 cagccacaaa gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca
     3721 gccaggctaa cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca
     3781 cgctactgcg tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac
     3841 gggcgatcat cgttgctgcc ggtaaggtca cccaccagct gtggtaatgc tcggttacca
     3901 gcaccatggc gatagccacg agaactttat ctgcgacagg gtcaaggaaa gcaccaaacc
     3961 gggtactctg gttccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga
     4021 cgcagaaaat gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata
     4081 caaagaatgg gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca
     4141 taatgacggg taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca
     4201 atgtttcaac gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc
     4261 aatttcctcg acgctggcgt tacgtaaacc ttgcaaaccg cccatatatt tcaacaacat
     4321 ttgccgacgt tttgccccga cgccttcaat ggtttccagg gaactggtat ttttgacctt
     4381 cgcccgtttt ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg
     4441 gataacatgc agcgcgggag aatctggcgg caaactaaat ccctcacctt ccggctcaaa
     4501 gaacagcgtt tccagcccag ccttacgatc tgctcctttg gcaacaccaa gtagcagcgg
     4561 atgattttta tcccatgaga catccagttc ggcgaaaaca tttttcgcct gcgcaagctg
     4621 gcctttgccg ccgtcaataa ggatcacatc cgggatctta ctgtcgtcga tagctttacc
     4681 ataacgccga cgcagcacct gattcatcgc cgcgtaatca tcgcccggcg tgatgccagt
     4741 aatgttatag cgccgatact ccgcacgcag cgggccgttg gcatcaaaca ccacacagga
     4801 agcgacggtt tgttccccca tggtatggct gatgtcaaag cactccatcc gcttcacttc
     4861 cggcaatttc aacacgctgg caagcgcggt cagccgctgg tgaacggtag attgctgcga
     4921 aagtttgctg gttaaggccg tcgccgcatt ggtacgcgcg agtttcagat aacgcgctct
     4981 atcgccacga ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc
     5041 ggcgagcagc gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg
     5101 catctggctg ccttgtaaat agaactgacc tacgaaggtt tccaccacct cgctcagttc
     5161 cgtaccgcca ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat
     5221 gaacaatacg tggacacaag ccatacccgc atcgaacgcc actccaataa cgtcaaggtc
     5281 gtcgccggta ttggaaacaa actgtttttc ggtgacacgt cgcaccgcct gaatttggtc
     5341 gcgaatccgc gccgcttctt caaactccag attctggctg gcagtttcca tacgactaat
     5401 gagttgcgta agcacctgat catctttgcc agacaaaaac aagcgcacat actcgacctg
     5461 ctgagcgtat tcttcttcac tcaccagtcc ttcaacgcac ggccccagac agcgccctat
     5521 ctgatattgc agacacggac gcgagcgatt gcgataaaca ctgttttcgc actggcgaat
     5581 gggaaaaatc ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg
     5641 gccgaaatat tcacctttgg catgcttcgc tccacgatgc atcgccagac gcgggtgggt
     5701 atcgccactc aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg
     5761 cggctgatag agtttgatgt agttgtgttc cagtaacagc gcttcggttt ctgtatgagt
     5821 aaccgttaca tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag
     5881 gttgctacgg aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat
     5941 aaccgtacca ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa
     6001 aaacgctttt gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga
     6061 ccatggcgaa ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac
     6121 atacgatagc ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca
     6181 ttgaccttct ggcccttggt gatcatcagc ataatctgca attcacgttc agacaaactg
     6241 gcaaatgggc tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgata
     6301 tcagaagcaa tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc
     6361 ggagccgcgc ctttgctgag gtagcccgca gctccggcct gcatgacttt cgctggtaaa
     6421 gggttttctg tatggacggt aagcatgatg attttgacat cagctgtgga acgcgcgatt
     6481 ttacgcgtcg cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca
     6541 acggcatttg cccggcacca tttaacggcg tcttcaccgc acgatgcctc accgacgact
     6601 tttataccct ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca
     6661 tcaacaagta gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata
     6721 gttagtcatt cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt
     6781 attcgatttt tctctcgttt ttgtcaattc gattgtcc
//
LOCUS       DADSZD010000203         6766 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.205, whole genome shotgun sequence.
ACCESSION   DADSZD010000203 DADSZD010000000
VERSION     DADSZD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.205"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(100..768)
                     /gene="artM"
                     /locus_tag="J1Z28_003979"
     CDS             complement(100..768)
                     /gene="artM"
                     /locus_tag="J1Z28_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBA7718755.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(768..1484)
                     /gene="artQ"
                     /locus_tag="J1Z28_003980"
     CDS             complement(768..1484)
                     /gene="artQ"
                     /locus_tag="J1Z28_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBA7718756.1"
                     /translation="MNEFFSLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1491..2222)
                     /gene="artJ"
                     /locus_tag="J1Z28_003981"
     CDS             complement(1491..2222)
                     /gene="artJ"
                     /locus_tag="J1Z28_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7718757.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSIDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2240..2968)
                     /gene="artP"
                     /locus_tag="J1Z28_003982"
     CDS             complement(2240..2968)
                     /gene="artP"
                     /locus_tag="J1Z28_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBA7718758.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3186..3701)
                     /locus_tag="J1Z28_003983"
     CDS             complement(3186..3701)
                     /locus_tag="J1Z28_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBA7718759.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3827..4150
                     /locus_tag="J1Z28_003984"
     CDS             3827..4150
                     /locus_tag="J1Z28_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBA7718760.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4147..4977
                     /locus_tag="J1Z28_003985"
     CDS             4147..4977
                     /locus_tag="J1Z28_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBA7718761.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4974..5987)
                     /locus_tag="J1Z28_003986"
     CDS             complement(4974..5987)
                     /locus_tag="J1Z28_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7718762.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6086..>6766)
                     /locus_tag="J1Z28_003987"
     CDS             complement(6086..>6766)
                     /locus_tag="J1Z28_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBA7718763.1"
                     /translation="TSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDAVRRT
                     LKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLTALWRVVNQIGGKE
                     RYFFGNILWQTRALMDLAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQLTLLFG
                     MKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMAKRIA
                     RLAEQSTD"
BASE COUNT         1634 a         1768 c         1802 g         1562 t
ORIGIN      
        1 gacagcttag tgggttgtcc gtcttcataa taagaattta tgcactatgt aggccggata
       61 aggcgtccac gccgcatccg gcatcagcat gcagctacat taattccgcc gttcgaatgc
      121 cagtgcttta cgctcgatca gacgcatcat cagcgtcagc aggccgttga cgaccaggta
      181 aataatcccc gccgcgccaa ataccattac atcgtaggtg cgtccgtaca acaactggct
      241 gtatcccatc acttccatga gcgtaatggt gtaagccaga gaggtacttt tgaacaccag
      301 caccacttcg ttggaataag aagagagcga gcgtttaaac gcgtacggca gcaggatcgc
      361 cagcgtatct tttttgctca ttcccagggc gctacaggac tgccattgac cttccgggat
      421 cgcgcgaatc gcaccgtaaa acagctgcgt ggtgtacgcc gcactgttca gcgacaacgc
      481 aatcagcgca cataaccacg gttctgacaa caaatgccac agtgccggat actcctgcaa
      541 cgtcgggaac tggcccggcc cgtaataaat caggaagatc tgcaccagca gcggcgtacc
      601 ggtaaacagc gtgatatagc cccgtaccag ccacaccagc accggcgttt tcagcgtcag
      661 gatgatggta aaaatcaatg ccagaatcag tgccacaatc agcgaggcaa cggttagcgt
      721 caggctggtg tgcagccctt tcatcagttc gggtaaatac tcaaacatta gctgggcctc
      781 cgctcaaaac gtgtcgcgcg caggtcaatg cgtttgagaa tgtactgact gagcagggtg
      841 atcaccaggt aaatcgccgc cgccacaatg taccaggtaa acggttcctg ggtacgggta
      901 gcgatgcttt tggtttgtag cattaaatca ttcacactaa tcaaactgac cagcgcggta
      961 tctttcagca gcaccagcca ctggttaccg agacccggca gcgcatgacg ccacatctgc
     1021 ggcatcacca gacggaaaaa gatagccgat ttcgacagcc ccagcgcctg accggattcc
     1081 cactgaccca ccggcaccgc ttttaatgca ccacgcagcg tttgcgaggc ataggcggca
     1141 tacagcagtg acagagcgat gacaccacaa aggaacgggc taacgtcgaa gttctcaatg
     1201 tccatctgca ccgggatctg cacgaaccca agattgatag tgaagccatc cgaaagcgtc
     1261 agcagcagct gcgaggagcc aaaatagata aacagcacca ccagaatttc tggcaggcca
     1321 cgcagaatgg ttaccagcgc tgaacctgcc cacgcgacag gacgccattt cgcagactcc
     1381 cataccgcaa agaacatcgc cagcgccagc ccgacaatca atgcacaaac ggcaaggccg
     1441 acggtcatcc cggcggcgct tgctaaagaa aaaaattcat tcatcaggaa ttacttctgg
     1501 aaccatttgt tgtagatggt ttcgtaagtg ccatctttct tcactttttc cagcgcagtg
     1561 ttgagtttct gctgcagttc agtgttgccc tggcgtactg cgatgccgag gccagtaccg
     1621 aagtaatctt tatcggtcac tttatcgccc actgccgcca gtttcgggtt atctttcagc
     1681 cattcagtta ccactgcggt gtcaccaaat acgctgtcga tacgaccgtt ttgcagatcc
     1741 agttttgcgt tctggtaact gtcatatggc acggtagtga tttccggatg cttatccata
     1801 atgaacttct ggtgtgtcgt cccgttctga acgccaactt ttttgccttt cagctgatca
     1861 atactggtgt atttgccttg ctgacccaca aacagggcag agttgtcata gtacggggtg
     1921 gtaaacagca cctgcttttc acgctccgga gtgatatcca tgcccgccat cactgcttct
     1981 acgcgacgga atttcaggct tgggatcagg ctgtcaaacg cctggttaga gaaagtgcag
     2041 gttgcatcaa tctctttaca caatgcctgc gccaggtcga catcaaaacc aacgatctgg
     2101 ttgtttgcat caatcgattc aaacggagga taggaggctt cggtggcaaa acgaatggtt
     2161 tcggctgctg tggcggaaag actaaaacct gcaattaacg cggcaatcag aacttttttc
     2221 attgttgtta tcccgaatct tagtgagaga gatagttttt aaatgcttcg gtttgcggct
     2281 cggtaaagca gctcgcgtcg ccttgttcta cgatatgacc gttttccata tacaccactc
     2341 ggctggcggt tttacgcgcc acttcaactt cgtgggtgac gatcacctgg gtaatattcg
     2401 tttctgccag ctcacgaatg atgctgacga tttgtgccgt aatttcgggg tccagtgcgg
     2461 cggtcggttc atcgaacagc agtacctgcg gttccatcat caacgcacgg gcaatagcaa
     2521 cacgctgctg ctgaccacca gaaagatgca gcgggtaacg atcgctataa ggtttgagac
     2581 gcagacgttc cagcagtttt tctgcacggg ccagcgcctg atctttactc aaccccagta
     2641 cacgacaggg cgcttcaatc aggttttgct gcacggtcag atgcggccac aggttgtatt
     2701 gctgaaacac catgccaacg ttacgacgca aatcgcgaat cgctttgtca gagggggttt
     2761 tggtgaaatc gaaatggttg cctgcaatgt tgagcgtacc ggagcgcggc atctcaagca
     2821 gattgagtac acgcagcagc gagcttttac ccgcgccgct ggggccaagt aacaccagcg
     2881 tttcgccctg tgggcaatcc agcgtgatat cgaacagcgc ctgatgcgcg ccgtagaagc
     2941 aattaatgcc gtttaattga atactcattg acactcgtat actggcagtc tgatagctat
     3001 tgaggtcgaa gatagtacct ttgacagaat aattatgcaa tatttctgct ttaaaagtta
     3061 aaagcaaagc gcattattca ataaacatag cacaaaataa cgggggcggt ggtcggcgag
     3121 cataaatgtc ggcattcctc acgaaatgcc ggacaattta cggggtttat tggttgatca
     3181 agacgttagc gattctcgat ggactgacgg agcgtacccg ctgtggcatg aacgctaccg
     3241 cctaagtagc gcacatcgtc gatgacccaa cactggcctt cctgaatcat taacacttca
     3301 tcctgccagc cctggtcacc ctgcttgaga tccacgcgca atggaatgtt acgggcatca
     3361 cgattaggga tagtcgatgc actggctacg tgggcgctgt ctggcaaggt ggttcgactg
     3421 gagaacggat cgctgcttaa caattcacga tgactattat cgcggctggc atcgctaagt
     3481 agggttgcca gtttgtcact caaatagggg cgcagagcgg taatgtcatt gctgcgatgt
     3541 tgaatgcggt agtcatagaa ctgttgagcc acgctatcag gccccccctg gacacaggaa
     3601 ccggtgcggg ggccgttatc tttataagct ggcgtgacgg tggtacaggc actgagtagc
     3661 agtgcgcaag ggataagcat ggtcaatttg ctatagcgca tgatgatttc cttataagcg
     3721 aacaccttga aagcgttcta cgataataat gatatccttt caataatagc gtatcagtcc
     3781 gataatgctt tgaagatcga aggcttagca aacaaggaga tcgatcatgc aattttctac
     3841 aaccccaact ctggaaggcc agaccatcgt tgaatattgc ggtgtggtga ctggcgaagc
     3901 gattttaggc gcgaatattt tccgtgattt ctttgccggt atccgcgata tcgttggcgg
     3961 acgctccggt gcctacgaaa aagaactgcg taaagcgcgg gagattgcct ttgaggagct
     4021 cggctcccag gcgcgagcgc tgggggctga tgccgtcgtc ggtattgata tcgactacga
     4081 aacggtcggg caaaacggca gtatgctgat ggttagcgtc agcggtacgg cggtgaaaac
     4141 gcgtcgatga gaagagtctt ctggctggtc gctgccgctc tgttactggc agggtgcaca
     4201 ggcgaaaaag gcattgtcga gaaagaggga tatcagcttg atacccgacg ccaggcgcag
     4261 gcggcgtatc cgcgcattaa agtgctggtg atccactaca ccgcagatga ttttgatagc
     4321 tcgctggcga cactgaccga taagcaggtc agctcgcatt atctggtccc tgcggtgcca
     4381 ccgcgataca acggtaaacc gcgcatctgg caactggtgc cggaacaaga actggcctgg
     4441 catgcgggga ttagcgcctg gcgcggggca acgcgcctta acgacacctc tattggcatt
     4501 gagctggaaa accgtggctg gcaaaaatcg gcgggagtga aatattttgc cccgtttgaa
     4561 cctgcacaga ttcaggcgct tattccgctg gcgaaagata ttattgcccg ttatcacatc
     4621 aagccggaaa acgtagtagc acatgcggat atcgccccgc agcgcaaaga cgatccgggg
     4681 ccattatttc cctggcagca actggcacag caggggattg gtgcctggcc ggatgcgcag
     4741 cgggtgaact tttaccttgc cgggcgcgcg ccgcacacac cggtagagac cgcgtcattg
     4801 ctggagcttt tggcgcgtta tggttatgac gtaaagcctg atatgactcc gcgcgagcag
     4861 cggcgcgtga ttatggcgtt ccagatgcat ttccgcccga cgttatataa cggcgaagcg
     4921 gatgcagaaa ctcaggcgat tgccgaagcg ttgctggaga aatacgggca ggattagcgc
     4981 ggcagttttc cgtggtcgcg tagccaggcg gcagttttct cgataccttc atccagggtg
     5041 aggactggtt gataacctaa ctcttcctgc gcacgcgtaa tatccagcgt aaagtcaaaa
     5101 ttcaacttgg agacgccgta gtgggtcagc ggcggctctt ttgctgactt gcggcctaaa
     5161 cgctccatgc tgcgggcgat catatccagc atcgggtagg ggacggaacg aatacgacag
     5221 tcaatattca actcgtcgat cagcttctgc acgatgctgc gcagtgtgcg atgctcgccg
     5281 ttggtgatgt tgtacacacg cccggaaggt agcttatcgc aggcttcctg gcttgccagc
     5341 cacatggcgt gcacggcatt ttcatagtag gtcatatcga ccagcgcact gccgccgtgt
     5401 ggtaacagaa tgctgccgta gtggtgcatc atatgcgcca gacgggggat aaagacttta
     5461 tcgtgcggtc cgaacagact ttgtgggcgc agaatagtaa agcgcgtttg tggattcgcc
     5521 tgcgaaagca tattgatcac ttcttcactg gctgctttgc tgcgggcaaa ctcgttggcg
     5581 aagcggtgag ggcgaaaatc ttctttaata tcgcgatggt ggtgataatc gaagtacagg
     5641 gagggagaag agatatgaat aaagttacgc acaccccaag cgacagccca ttcacccagg
     5701 cggcgagtgg cgcgaacgtt agccagatcg aaagcctgtt gtgtccccca gggcgaggta
     5761 aagctggagc agtgccacag cgtatcaatg cccgcgagca tcactttagc ttgtgatgag
     5821 accagctcgg tcagatccgc cggaacaaac tctgcgccca ttttttccag caatttaccc
     5881 attgcctcgt tgcgaccggt cgctcgcacg ctgatgcctt tctggcataa aaactctacc
     5941 gcgtttcgac ctaagccgct ggtggcgccg gtaaccagta ccttcatatc aatccactgt
     6001 tgttgagaaa atgacgtgcg cattcttccg tgatttcccc cttgatgcaa tgggaaacat
     6061 gaaagaataa cgcaggtttt gtcgattaat ctgtgctttg ttctgccagt ctggcgattc
     6121 gttttgccat tccgcgaaaa ataaacagat gcgcggggat catcaataac cagtaaaaca
     6181 gccccggcat accgtgcgga tgccagaaag cacggacatc aatagtacga tagtcgcctt
     6241 tatcttccag ggtaaaacac agtcgtccca gccccggcgc tttcatgcca aataacaacg
     6301 taagttgttt ttccggttca acgacaatga ctttccagct atccaccgca tcgccggtct
     6361 gtaaatattc gcgctccggg cggcctttcg ccagcttatg accgatcgca aggtccatca
     6421 acgcccgtgt ctgccacaaa atattgccaa agaaataacg ctctttaccg ccgatttggt
     6481 tcactacccg ccataaagct gtaaggctgg cggacgtttt aacggtaaac cccgcctgtt
     6541 tggcgaaata accgtactcc ggtcgccagc gggcaaaggc ctgagcgtcg tagccccagt
     6601 cgctggagtt gaccagcttt tcttcttctt tcagggtgcg acgtaccgcg tcatcgaaag
     6661 cgatcagccg ttgtgggatg agcgcacgta gcgcggtgtc atccgccagc agatcgtgtt
     6721 tcagcccctg aatcaacgcc ctggcggtgg tgggcggtac tgaagt
//
LOCUS       DADSZD010000204         6683 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.206, whole genome shotgun sequence.
ACCESSION   DADSZD010000204 DADSZD010000000
VERSION     DADSZD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.206"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..590)
                     /locus_tag="J1Z28_003988"
     CDS             complement(<1..590)
                     /locus_tag="J1Z28_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HBA7718764.1"
                     /translation="MSLIKAGNDSGGRDAINRLIKAYNFSSRQQLCEHLEVSKSTMAN
                     RYLRDSFPAEWVIQCALETGVSLLWLATGQGDMYACENEETNLKNESFVTVRPLSKIV
                     APSIKRVELKNGELQPSDEILLDSSLLDGDSSNALFVKTANDSFVVDTSVKQVSNGFW
                     LVDMDGVKSIVKIARIPGNKIVVHQDETSFECSVDD"
     gene            707..949
                     /locus_tag="J1Z28_003989"
     CDS             707..949
                     /locus_tag="J1Z28_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBA7718765.1"
                     /translation="MSTDISIRVPKEMATPAEFAEWEGISRGSVYQKIHHGQLAKYMV
                     KKEKNKGRVSLRYLMYKTDQVRESLGHSNFRVIVGK"
     gene            982..1491
                     /locus_tag="J1Z28_003990"
     CDS             982..1491
                     /locus_tag="J1Z28_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HBA7718766.1"
                     /translation="MFDYKISKHPHFDEACRAFALRHNMAKLAERAGMNVQTLRNKLN
                     PDQPHQLNAPEIWLLTDLTEDSTLIDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTMT
                     GLGASFGLL"
     gene            1499..1699
                     /locus_tag="J1Z28_003991"
     CDS             1499..1699
                     /locus_tag="J1Z28_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HBA7718767.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            1663..2004
                     /locus_tag="J1Z28_003992"
     CDS             1663..2004
                     /locus_tag="J1Z28_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HBA7718768.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINSEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            2072..2305
                     /locus_tag="J1Z28_003993"
     CDS             2072..2305
                     /locus_tag="J1Z28_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HBA7718769.1"
                     /translation="MRNIETLTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            2305..2532
                     /locus_tag="J1Z28_003994"
     CDS             2305..2532
                     /locus_tag="J1Z28_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA7718770.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            2529..3383
                     /locus_tag="J1Z28_003995"
     CDS             2529..3383
                     /locus_tag="J1Z28_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HBA7718771.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCEAFISRARALFEEAKREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLKVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASILEHRSSEGHPVIV
                     SNSNTSLTRSLYRNFTHHCIRAKRSIGVAAGEGKFATEMIATKSANWFSADFSRGRDS
                     TVIFGVQV"
     gene            3389..4210
                     /locus_tag="J1Z28_003996"
     CDS             3389..4210
                     /locus_tag="J1Z28_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718772.1"
                     /translation="MHHGIHHFHGTPVWGSAGDVHRIAVSGAGAFVSYVRPDQIAASI
                     QHAQVVGIDNGAFSAWVRGLKINWSDFYKWLLNYYHHPKVAFFVIPDVVDGGERDNDA
                     LINEVPKMFYGKATPVWHLHESIDRLIELCREWPRVCFGSSGEYAAIRTAHWHRRMQD
                     AFEAIYCRHNFKTAVHGLRMLDGRVLGNYPLATADSTNLACNVPKFNSKYPELTRAIQ
                     EAEYSRNLTEKELKAVILKNRCAILKGAIEAVRPPSVSDWLSNGLQPSQLELEIA"
     gene            4210..6582
                     /locus_tag="J1Z28_003997"
     CDS             4210..6582
                     /locus_tag="J1Z28_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HBA7718773.1"
                     /translation="MNYSYSWNAEKKAINPYVETEEQSSVSALSNLIALYAADNEQEQ
                     LRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQRFNPDMVILADVNAQ
                     PSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRDCQLSTSFRFMASHE
                     GLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYVTDDVKPEEIRKTWEKVAA
                     ETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWYRKLWKMRCEWREEQ
                     LRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDGDTLDMEDVVNASSS
                     NPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNNGRPNPTWTNATVRQ
                     SSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCFMRKKDRRAITALLR
                     KFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNIDGRGLAGEISKETGK
                     SLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAARQQGDKKAGAPVLDN
                     PRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEPTAYGDHGIRIYGIW
                     SPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCPLAENLKQQGKDKSA
                     DGDTRTDITRMDDKELHDYLHSMSKKERRELAARLRLVKPKRRKDYKQRITDHQRQQL
                     VYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDDKWRNLY"
BASE COUNT         1777 a         1543 c         1698 g         1665 t
ORIGIN      
        1 atgtcgtcga ccgagcattc aaatgatgtt tcatcttgat gaactactat tttattacca
       61 ggaatgcgag cgattttgac gatgctttta actccgtcca tatccactaa ccagaagcca
      121 ttactgactt gtttaacaga cgtatccact acaaagctat cattagctgt tttaacaaat
      181 aaagcgttgg atgagtcacc atcgagcagg ctgctatcaa gaagaatttc atcacttggt
      241 tgcagttcgc cgttcttcag ttcaacacgt ttgatgctag gagcaacgat cttagaaagt
      301 ggtcttacag tgacgaagga ttcatttttg agatttgttt cttcgttctc acacgcatac
      361 atatctcctt gaccagtagc cagccataga agggaaactc ctgtttcaag ggcacattga
      421 attacccatt cggcaggaaa gctatccctt aagtatctgt tagccatagt gctttttgat
      481 acttccagat gttcgcagag ctgctgtcgt gagctgaagt tgtaagcctt aataagccta
      541 ttaatcgcat cgcgcccacc actatcattc cccgccttga ttaaactcat aatcaaacct
      601 cttgacgtat ataaaaagtg atcctaatat ccattcatgg tttgaaaagc aaaaccaaac
      661 cacataaaac aagataaaac gaaaccaaac taagagatac tgcactatga gtactgatat
      721 ttcaattcgt gtaccaaaag agatggctac gcctgcagag ttcgcggaat gggaaggtat
      781 ctcccgcggc tccgtgtatc aaaaaattca ccatggtcag cttgctaaat acatggtcaa
      841 gaaagaaaaa aacaaaggcc gcgtaagcct gcgttattta atgtacaaaa ccgatcaggt
      901 ccgtgaatcc ctcggtcatt ccaacttccg cgtcattgtt ggtaagtaag ttcaattatg
      961 agaactttct aagggggtag catgtttgat tataagattt ccaaacaccc gcattttgat
     1021 gaagcctgta gagcttttgc acttcgtcac aatatggcga agctggcaga acgtgcagga
     1081 atgaatgtcc agactttgcg aaacaaactc aacccagatc aaccgcatca gctaaatgcg
     1141 ccagaaatct ggctgcttac cgatctgact gaagattcaa cgctgataga tggttttctg
     1201 gcacagattc actgcctgcc atgtgtaccg attaatgagg tagcaaaaga gaaactgccg
     1261 cattacgtca tgagtgcaac cgcagagatc gggcgtgttg ctgcaggtgc ggtatctggc
     1321 gatgtaaaaa ccagtgcagg tcgtcgtgat gctatcagca gcattaactc tgtaacacga
     1381 ctgatggcgc tggctgctgt ttcattgcag gcccgtttac aggctaaccc ggcgatggcg
     1441 agtgcagttg ataccatgac tggccttggt gcttcattcg gtttgctgtg aggtgcttat
     1501 gcttacgaaa gaaccatcat ttgcatcgct gctggttaaa caaagcccgg caatgcacta
     1561 cggtcacggc tggatcatgg gtgaggatgg taaacgctgg catccgtgcc gttcacaaga
     1621 tgaattgctg gcagaactat ctacgaaaaa acgggggaac aaatggctat tgaaggcgct
     1681 gcggcgactg ttccattaag ccccggtgaa cgcctgaatg gacttaatca tattgcggag
     1741 ttaagggcga aagtttttgg tcttaatatt gagtcagagc ttgagcggtt tattaaagat
     1801 atgcgtgatc cacgggatat taatagtgaa caaaataaac gggcactggc tgccatattc
     1861 tttatggcaa aaattccagc tgaacgtcat agcatcagca ttaatgagct gaccactgac
     1921 gaaaagcggg agttgattaa agcaatgaat cattttcgtg cagtggtgag cttatttccc
     1981 agacggttaa ccatgccgaa ttaaccaact aatgaaatta atggcgtaaa cccgccgggc
     2041 atccctttat ctaaattcag gagaattgat tatgcgtaat attgaaaccc tcacgactaa
     2101 aaccggaccg gatgacgcag ggcttaatat tttactgaca gaggctcgtc tggaagaacg
     2161 ccgggcaagg gctgaagcaa tggcagctcg ccttgatagt ctggcgtgtc atattacatc
     2221 ccgccagcta aaccacgtcg aagcggcaga actgctgcgt gtgactgctg aagcaatcca
     2281 gaacgaagcg caggagatcc actaatggct gatgcaatgg atctcgtaca gcagcgcgtt
     2341 gaagaagaac gccagcgcca tatccgtgct gcccgtgcca aaacaccggg cgtgtcacgc
     2401 gtactttgca ttgaatgtga agcgccaatt ccgccagcac gacgccgcgc cattccggga
     2461 gtgcagcttt gcattacctg tcaggaaatc gcagaactga aaggcaaaca ttacaacgga
     2521 ggtgctgtat gagtaccatc ctgaaatggg cgggtaataa aactgccatt atgtccgaac
     2581 tgaaaaaaca tcttcctgct ggcccgcgac tggttgaacc tttcgcgggt tcctgtgcag
     2641 tgatgatgga gacggattat cccagctatc ttgttgcgga tattaatcct gatttaatca
     2701 acctctataa aaaggttgct gctgattgtg aggcgtttat atctcgtgcc agagctttat
     2761 ttgaggaagc aaaaagggag gtggcttatt acaacataag gcaggagttt aattactcca
     2821 ctgaaattac tgatttcatg aaagcggtat atttcctgta tctcaatcgt cacggttacc
     2881 gtggtttatg tcgctataac aagagcgggc atttcaacat tccctacggt aattataaaa
     2941 atccgtattt ccctgaaaaa gaaattcgcg catttgcaga gaaagcccag cgggcaacgt
     3001 ttatctgcgc cagctttgat gaaacgctgg cgatgttgaa ggtgggggat gtggtgtatt
     3061 gcgatccgcc ttatgacggt acgttttccg gctatcacac tgatggtttc actgaagatg
     3121 atcagtatca cctggcatcc attcttgaac atcggtcatc agaaggacat ccggtcattg
     3181 tttctaacag taacacgtct ttgacccggt ccctttatcg taatttcact caccactgca
     3241 tcagggcgaa acgcagcatc ggcgttgcag cgggggaggg aaaatttgca acagagatga
     3301 ttgccactaa atctgctaat tggtttagtg ccgattttag taggggacgt gactctactg
     3361 ttattttcgg ggtgcaagtg tgaaagaaat gcaccacgga attcatcatt tccatgggac
     3421 gcctgtctgg ggaagtgctg gcgacgttca tcgtattgcg gtgagcggag ctggcgcttt
     3481 cgtctcctat gtacgaccag atcagattgc ggcgtccatt cagcacgctc aggtcgtcgg
     3541 cattgataac ggcgcatttt ctgcatgggt gcgtgggcta aaaattaact ggagtgattt
     3601 ttataaatgg ctcctgaact attaccacca tcctaaggtc gctttttttg tcattcctga
     3661 tgttgtggac ggaggtgaac gtgacaatga tgccctgata aacgaagttc cgaaaatgtt
     3721 ctacgggaag gcaactcccg tctggcatct gcacgagtca atcgatcggc ttatcgagct
     3781 atgtcgtgaa tggcctcgtg tctgctttgg atcgtctggt gaatatgcgg ctatcagaac
     3841 tgcgcactgg catcgtcgta tgcaggacgc ttttgaagca atttattgcc gacacaattt
     3901 caaaacagct gttcatggtt tgcgcatgct tgacggtcgt gtgttgggaa attacccact
     3961 ggcgactgcc gacagtacaa atcttgcctg caatgtcccc aaatttaata gcaaatatcc
     4021 tgagctgacg cgggctattc aggaggctga gtattcgcgc aatctgacgg aaaaggagct
     4081 gaaagctgtc attctgaaaa accgttgcgc aattttaaaa ggtgcaattg aagctgttcg
     4141 cccaccttca gtttctgatt ggctgtcgaa tggtttgcag ccttcacagc tcgaactgga
     4201 gattgcgtaa tgaactacag ctattcctgg aatgctgaga aaaaagcaat caatccttac
     4261 gtagagacag aagagcaatc ttcagtttct gcgctttcaa acctgatcgc tctgtacgct
     4321 gccgataacg agcaggaaca gctgcgccgc gaggcactga gtgatcaggt ctgggagcgt
     4381 tatttcttta atgaatcccg tgatcctgtc cagcgcgaaa tggagcagga taagctcatt
     4441 agtcgggcaa agctggcgca tgagcagcag cgttttaatc cagatatggt cattctggcg
     4501 gacgtcaatg ctcagccttc ccatatcagc aagccgctga tgcaacgtat tgaatacttc
     4561 agcagcctgg gcaggccaaa ggcttattcc cgctatttgc gtgagacgat taagccatgt
     4621 ctggaacggc tggagcatgt acgcgactgt cagctatcca cttcttttcg ctttatggca
     4681 agccatgaag ggctggacgg cctgctgatc ctgcctgaaa tgagtcagga tcaggtgaaa
     4741 cgcctgtcca ccctggtagc tgcgcatatg agcatgtgcc ttgatgcagc ttgtggcgat
     4801 ttgtatgtca ccgatgacgt taagccagaa gaaatccgca agacatggga aaaggtggca
     4861 gcggaaaccc tgcgtctgga tgtcatcccg cctgcgtttg agcaactccg ccggaaaaga
     4921 aaccgccgta aacccgtgcc ctatgaactc attccgggtt cgctggcgcg tatgttgtgc
     4981 gctgactggt ggtaccggaa attatggaag atgcgttgcg aatggcggga agagcagttg
     5041 cgtgctgtct gcctggtcag caaaaaagca tctccctatg tcagctatga agccgtgatg
     5101 cataaacgtg agcagcgccg taagtcgctg gagttttttc gttctcatga actggtgaac
     5161 gaagacggcg acacgctgga tatggaagac gtggtaaacg ccagcagcag caaccctgcg
     5221 catcgccgca atgagatgat ggcctgtgtt aaaggcctgg agcttatcgc ggaaatgcgc
     5281 ggtgactgcg ccgttttcta caccatcacc tgtccgtcac gtttccattc cacgctaaat
     5341 aatggcaggc ccaacccaac ctggacaaat gcgacggtaa gacaaagcag tgattatctg
     5401 gtcggcatgt ttgctgcatt tcgtaaggcg atgcacaaag ccggattgcg gtggtatggc
     5461 gtgcgggtgg ctgagccgca tcatgacggt acagttcact ggcacctgtt gtgttttatg
     5521 cgcaaaaaag accgccgcgc catcactgca ttactgcgta agtttgccat ccgtgaagac
     5581 cgcgaggaac tgggcaataa cactgggccg cgctttaagt ctgagttgat taacccgcgc
     5641 aaaggtacgc caacaagcta catcgcgaaa tacatcagta agaacattga cgggcgtggt
     5701 ctggctggcg agatcagcaa ggaaacgggg aaatccctgc gtgataatgc tgaatacgtt
     5761 aatgcctggg cgtctctgca tcgtgttcag caattccgct tctttggcat tccggggcgt
     5821 caggcttacc gtgaactgcg attgctggct ggtcaggcgg caaggcaaca gggggacaaa
     5881 aaagcaggtg cgccggtact ggataacccg cgccttgatg ccatcctggc tgctgctgat
     5941 gctggttgtt ttgccaccta catcatgaag cagggcggcg tactggttcc ccgcaaatat
     6001 cacctcatca gaaccgctta tgaaatcaac gaagagccga ccgcctatgg cgatcacggc
     6061 attcgtattt atggcatctg gtcacctatt gcagagggca agatctgcac tcatgccgtg
     6121 aagtggaaaa tggttcgtaa ggccgttgat gttcaggagg cggcagccga ccagggcgct
     6181 tgcgcccctt ggactcgtgg caataactgt ccccttgctg aaaatttgaa acaacaaggg
     6241 aaagacaaat cagctgatgg ggataccaga acggatatca cccgcatgga tgacaaggag
     6301 ttgcacgatt acctgcacag tatgagcaaa aaagagcgcc gggaactggc tgcaaggtta
     6361 cgcctggtga aaccgaaacg gcgtaaagac tacaaacagc gaattacaga ccatcagcga
     6421 cagcagctcg tctatgaact gaagtccaga ggatttgatg gcagcgagaa agaggtcgat
     6481 ttactccttc gcggcggcag tattccgtca ggagcaggcc tgcgtatctt ctatcggaac
     6541 cagcgtttgc aggaagatga taagtggcga aacctgtatt aattacgcgg gttaacaatt
     6601 cgtgctctta ataataccag gcatatcagg ctgatgaacg taaaaaaacg ttttacatca
     6661 gtaagattat tatatactgt aaa
//
LOCUS       DADSZD010000205         6667 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.207, whole genome shotgun sequence.
ACCESSION   DADSZD010000205 DADSZD010000000
VERSION     DADSZD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.207"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            82..1806
                     /gene="aceK"
                     /locus_tag="J1Z28_003998"
     CDS             82..1806
                     /gene="aceK"
                     /locus_tag="J1Z28_003998"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HBA7718774.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(1823..2647)
                     /gene="iclR"
                     /locus_tag="J1Z28_003999"
     CDS             complement(1823..2647)
                     /gene="iclR"
                     /locus_tag="J1Z28_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBA7718775.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            2847..6530
                     /gene="metH"
                     /locus_tag="J1Z28_004000"
     CDS             2847..6530
                     /gene="metH"
                     /locus_tag="J1Z28_004000"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBA7718776.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWEMLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
BASE COUNT         1572 a         1734 c         1866 g         1495 t
ORIGIN      
        1 acgccgcatc cggcaatcag cgcctgatgt gacgcttacg cgtcttatcc ggcaattctc
       61 tgctcccgac gagggcaaca aatgccgcgt ggcctggaat tactgattgc acaaaccatt
      121 ttgcaaggtt tcgacgctca gtatggtcga ttcctcgaag tgacttccgg ggcgcagcag
      181 cgtttcgagc aagccgactg gcatgctgtc cagcaggcga tgaaaaaccg tattcatctt
      241 tacgatcatc acgtgggtct ggtcgtggag caactgcgct gcattactaa tggccaaagc
      301 acggacgcgg catttttact gcgcgttaaa gagcattaca cccggctgtt gccagattac
      361 ccgcgcttcg agattgcgga gagctttttc aactccgtgt actgtcggtt atttgaccac
      421 cgctcgctta ctcccgagcg gctttttatc tttagctccc agccagagcg ccgctttcgt
      481 accattccac gcccactggc gaaagacttt caccccgatc acggctggga atctctgctg
      541 atgcgcgtta tcagcgatct gccgctgcgt ctgcgttggc agaataaaag ccgtgacatt
      601 cattacatcg ttcgccatct gacggaaacg ctggggacag acaacctcgc ggaaagtcat
      661 ttacaggtgg cgaacgaact gttttaccgc aataaggcag cctggctggt gggcaaactg
      721 atcacgcctt ccggcacgtt gccattttta ttgccgatcc accagacgga cgacggcgag
      781 ttatttattg atacctgcct gacgacgacc gccgaagcga gcattgtttt tggctttgcg
      841 cgttcttatt ttatggttta tgcaccgctg cccgcagcgc tggtcgagtg gctacgggaa
      901 attctgccag gtaaaaccac cgctgaattg tatatggcta tcggctgcca gaagcacgcc
      961 aaaaccgaaa gctaccgcga atatctcgtt tatctacagg gctgtaatga gcagttcatt
     1021 gaagcgccgg gtattcgtgg aatggtgatg ttggtgttta cgttgccggg ttttgatcga
     1081 gtattcaaag tcatcaaaga caggttcgcg ccgcagaaag agatgtctgc cgctcacgtt
     1141 cgtgcctgct accaactggt gaaagagcat gatcgcgtgg gccgaatggc ggacacccag
     1201 gagtttgaaa actttgtgct ggagaagcgg catatttccc cggcattaat ggaattactg
     1261 ctccaggaag cagcggaaaa aatcaccgat ctcggcgaac aaattgtgat tcgccatctc
     1321 tatatcgaac gacggatggt gccgcttaat atctggcttg agcaagttga ggggcagcaa
     1381 ttgcgtgatg ccattgaaga gtacggcaac gccattcgcc agcttgccgc tgccaacatc
     1441 ttccctgggg atatgttatt taaaaacttt ggcgtcaccc gtcacgggcg cgtggtgttt
     1501 tatgattacg acgaaatttg ctacatgacg gaagtgaatt tccgcgacat cccgctgccg
     1561 cgctacccgg aagacgaact tgccagcgaa ccgtggtaca gcgtctcgcc gggcgatgtt
     1621 ttcccggaag agttccgtca ctggttatgt gccgacccgc gtattggtcc gctatttgaa
     1681 gagatgcacg ccgacttgtt ccgcgctgat tactggcgcg cactacaaaa ccgcatacgt
     1741 gaagggcatg tggaagatgt ttatgcgtat cggcgcaggc agcggtttag cgtccgattc
     1801 gtataaatca gaggcgaaaa agtcagcgta ttccaccgta cgccagcgtc acttccttcg
     1861 ccgctttaat caccatcgcg ccaaactcgg tcacacggtc atcggtaata cgtgaaatcg
     1921 gtccggaaat tgaaattgcg gcaaacggtt cgcggtgctc atcgaaaata cacgctgcaa
     1981 ggcaacgcag ccccagcgca tgttcctcat cgtcaaatga ataaccccgt ttgcgcgttt
     2041 gggcgagatc ttcttttaaa tgcacaggag acaccagcgt cgcgtgggta taggcatgta
     2101 accctttgcg gtgcagcagc ttcgtcacct gttcttcgct cagttgggcc agaaaggctt
     2161 tacccgcacc ggaagcgtgc atcggcaatt taccgccgat aggcgcggac attcgcatca
     2221 ggtgagtaca ctgtacctgg tcgataataa tcgcttcgtg atcgctttga tcaagcaccg
     2281 ccatattgac cgtttcgcca gactcttcca tcagattgcg cagaatagga tggacaatcg
     2341 ctaacaaatt acggctctgg agaaagctgc tgccgaccat aaaggcgtgt gcgccgatcg
     2401 cccagtgacc cagttcgccg acctgacgga caaagccctg ttgctgcatc gtggttagca
     2461 ggcggtgggt cgtggaattg ggtaacccgg cttgttgcgc cagttccgtg agtgccacac
     2521 tgccattgga ttcggcaatc cactccagta atttcaggcc acgcgttaaa gactgaacct
     2581 gtccagtcgc tggtgcggtg gcaacggcgg gttttctgcc gcgttttgcg ggaatgggtg
     2641 cgaccatgac agtctccttt ttctgtatcg tggaaatcat tttcattttt attatcagcg
     2701 aatgcaatag ttgctgaaca gatccgatga gttaatgttg aacaaatctc atgttgcgtg
     2761 gtggtcgctt ttaccacaga tgcgtttatg ccagtatggt ttgttgaatt tttattaaat
     2821 ctgggttaag cgtgtcggga gcaagtgtga gcagcaaagt ggaacaactg cgtgcgcagt
     2881 taaatgaacg tattctggtg ctggacggcg gtatgggcac catgatccag agttatcgac
     2941 tgaacgaggc cgattttcgc ggcgagcgtt ttgccgactg gccgtgcgat ctcaaaggca
     3001 acaacgacct gctggtgctc agcaaaccgg aagtgatcgc cgctatccac aacgcctact
     3061 ttgaagcggg tgcggatatc atcgaaacca acaccttcaa ctccacgacc attgcgatgg
     3121 cggattacca aatggaatcc ctgtcggcgg aaatcaactt tgcagcggcg aaactggcgc
     3181 gcgcctgtgc cgacgagtgg actgcccgca cgccggagaa accgcgctac gttgccggtg
     3241 ttctcggccc gaccaaccgc acggcgtcta tttctccgga cgtcaacgat ccggcattcc
     3301 gcaacatcac ttttgaccag ctggtggcgg cttatcgaga atccaccaaa gcgctggtgg
     3361 aaggtggcgc ggacctgatc ctgattgaaa ccgttttcga cacccttaac gccaaagcgg
     3421 cggtctttgc ggtgaaaacg gagtttgaag cgctgggcgt tgaactgccg attatgatct
     3481 ccggcaccat caccgacgcc tccgggcgca cgctctccgg gcagactact gaagcgtttt
     3541 acaactccct gcgccacgcc gaagcgctga cctttggcct gaactgtgcg ctggggccag
     3601 acgaactgcg ccagtacgta caggaactgt cgcgtattgc ggaatgctac gtcaccgccc
     3661 acccgaacgc cgggctgccc aacgcctttg gtgaatacga tctcgacgcc gacacgatgg
     3721 caaaacagat acgtgaatgg gcagaagcag gctttctcaa tatcgtcggc ggctgctgcg
     3781 gcaccacgcc acaacatatt gcagcgatga gtcgtgcagt agaaggatta gcgccgcgca
     3841 aactaccgga aattcccgta gcctgccgtt tgtccggcct ggagccgctg aacattggcg
     3901 aagatagcct gtttgtgaac gtgggtgaac gcaccaacgt caccggttcc gctaagttca
     3961 agcgactgat caaagaagag aaatacagcg aggcgctgga tgtggcgcgt cagcaggtgg
     4021 aaaacggtgc gcagattatc gatatcaaca tggatgaagg gatgctcgac gccgaagcgg
     4081 cgatggtgcg ttttctcaat ctgattgccg gtgaaccgga tatcgctcgc gtgccgatta
     4141 tgatcgactc ctcaaaatgg gacgttattg aaaaaggtct gaagtgtatc cagggcaaag
     4201 gcattgttaa ctctatctcg atgaaagagg gtgtcgacgc ctttatccat cacgcgaaac
     4261 tgttgcgtcg ctacggtgcg gcagtcgtgg taatggcttt tgacgaacag ggccaggccg
     4321 atacccgcgc acggaaaatc gagatttgcc gtcgggcgta caaaatcctc accgaagagg
     4381 ttggcttccc ggctgaagat atcatcttcg acccgaacat cttcgcggtc gcaactggca
     4441 ttgaagagca caacaattac gcgcaggact ttatcggcgc gtgtgaagac atcaaacgtg
     4501 aactgccgca cgcactgatt tccggcggcg tttctaacgt ttctttctca ttccgtggca
     4561 acgatccggt gcgcgaagcc attcacgcgg tgttcctcta ctacgctatt cgcaatggca
     4621 tggatatggg gatcgtcaac gccgggcaac tggcgattta cgacgacctg cctaccgagc
     4681 tacgcgacgc ggtggaggat gtgatcctca accgtcgcga cgatggcacc gagcgtttac
     4741 tcgagcttgc cgagaaatat cgcggcagca aaaccgacga caccgctaac acccagcagg
     4801 cggagtggcg ctcgtgggaa gtgaataaac gtctggaata ctcgctggtc aaaggcatta
     4861 ccgagtttat cgagcaggat accgaagaag cccgccagca ggctacgcgt ccgattgaag
     4921 tgattgaagg cccgttgatg gacggcatga acgtggtcgg cgacctgttt ggcgagggca
     4981 aaatgttcct gccgcaggtg gtgaaatcgg cgcgcgtaat gaagcaggcg gtggcgtatc
     5041 tcgaaccgtt tattgaagcc agcaaagagc agggtaaaac caacggcaag atggtgatcg
     5101 ccaccgtgaa aggcgatgtc cacgacatcg gtaaaaatat cgttggcgtg gtgctgcaat
     5161 gtaacaacta cgaaattgtc gatctcggcg tgatggtgcc tgcggaaaaa attctccgta
     5221 ccgctaaaga agtgaatgct gatttgattg gcctttcagg gctgatcacg ccgtcgctgg
     5281 acgagatggt taacgtggcg aaagagatgg agcgtcaggg cttcaccatt ccgctattga
     5341 ttggcggcgc gaccacctca aaagcgcaca cggcggtgaa aatcgagcag aactacagcg
     5401 gcccgacggt gtatgtgcag aacgcatcgc gcaccgttgg tgtggtggcg gcgctgcttt
     5461 ccgacactca gcgcgatgat ttcgttgctc gtacccgcaa ggagtacgaa accgtgcgta
     5521 ttcagcacgg acgaaagaaa ccacgcacac caccggtcac gctggaagcg gcgcgtgata
     5581 acgacttcgc ttttgactgg caggcttaca cgccgccggt ggcgcaccgt ctcggtgtgc
     5641 aggaagtcga agccagcatc gaaacgctgc gtaattacat cgactggacg ccgttcttta
     5701 tgacctggtc gctggccggg aagtatccgc gcattctgga agatgaagtg gtaggtgttg
     5761 aggcgcagcg gctgtttaaa gacgccaacg acatgctgga taaattaagc gccgaaaaaa
     5821 tgctgaaccc gcgtggcgtg gtgggcctgt tcccggcaaa ccgtgtgggt gatgacattg
     5881 aaatctaccg tgacgaaacg cgtacccatg tgatcaacgt cagccaccat ctacgccaac
     5941 agaccgaaaa aaccggcttc gctaactact gtctcgctga cttcgttgcg ccgaagcttt
     6001 ctggtaaagc agattacatc ggcgcatttg ccgtgactgg cgggctggaa gaggacgcac
     6061 tggctgatgc ctttgaagca cagcatgatg attacaacaa aatcatggtg aaagcgcttg
     6121 ccgaccgtct ggcggaagcc tttgcggagt atctccatga gcgtgtgcgt aaagtctact
     6181 ggggctatgc gccgaacgag aacctcagca acgaagagct gatccgcgaa aactaccagg
     6241 gcatccgtcc ggcaccgggc taccctgcct gcccggaaca tacggaaaaa gccacaatct
     6301 gggagatgct ggaagtggaa aaacataccg gtatgaaact cacggaatcc ttcgccatgt
     6361 ggccgggtgc atcggtttcg ggttggtact tcagtcatcc agacagcaag tattacgcgg
     6421 tggcgcaaat tcagcgcgat caggttgaag actatgcccg ccgtaaagga atgagcgtta
     6481 ccgaagtcga gcgttggttg gcaccgaatc tggggtatga tgccgagtaa gtgataacac
     6541 cggggtattt gccccggttt tttatgtcat aaataatgga ttattgaaca tgataaaaat
     6601 tgatgaaata gattttttgc tttcgaagga tgttcagagt ccgaagttac tgaactatta
     6661 ggaaaaa
//
LOCUS       DADSZD010000206         6660 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.208, whole genome shotgun sequence.
ACCESSION   DADSZD010000206 DADSZD010000000
VERSION     DADSZD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.208"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..525
                     /locus_tag="J1Z28_004001"
     CDS             <1..525
                     /locus_tag="J1Z28_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBA7718777.1"
                     /translation="LLSGVAFNALADDANQGSGKITFKGEVIDAPCSIAPGDEDQTIN
                     LGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVKITFSSASVDATDSNLLKN
                     TLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSYQELNFKARMESLGRTATP
                     GNVQAQANYVLDYK"
     gene            612..3134
                     /locus_tag="J1Z28_004002"
     CDS             612..3134
                     /locus_tag="J1Z28_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA7718778.1"
                     /translation="MNNKNTFSRDKLSHAIKNALSGVVCSLLFVLPVHAVEFNVDMID
                     AEDRENIDISRFEKKGYIPPGRYLVRVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTNFGLNTEFIESLQNIAGSECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDAGIAGFILDYNVYASQYAPHHGYSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSGNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSTSNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGIALHDYSYGDNAR
                     MMVDTDGISGIEINSNRTVTNGLGIAVIPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSTLHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3150..3890
                     /locus_tag="J1Z28_004003"
     CDS             3150..3890
                     /locus_tag="J1Z28_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7718779.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQIQIRLMALASLNDLPQD
                     RETLFYYNVREIPPRAKEQNVMQIAMQSRLKLFWRPKAIELKEGEMIPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFTSATQHLSSLPSTFQLGFVAD
                     YGGLEMFKVECNSIQSLCQSSPAKKGKI"
     gene            3887..4936
                     /locus_tag="J1Z28_004004"
     CDS             3887..4936
                     /locus_tag="J1Z28_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HBA7718780.1"
                     /translation="MITLFRLLAILCLFFNVSAFAVDCYQDGYRGTTLINGDLPAFKI
                     PENAQPGQKIWESGDINITVYCDNAPGWTTDNPSENIYAWIKLPQINSADMLNNPYLT
                     FGVTYNGVDYEGINERIDTHACLDKYEQYYNGYYYDPVCNGSTLQKNVTFNAHFRVYV
                     KFKSRPAGDQTVNFGIVNVLQFDGEGGANMSSNAKNLRYVITGLDNISFLDCSVDVRI
                     FPESQIVNFGQIAANSIATYRPKAAFSVSTIKDVAADCTEQFDVATSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNQYKRFTTYIPGQTGAIVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(4991..5644)
                     /gene="ribB"
                     /locus_tag="J1Z28_004005"
     CDS             complement(4991..5644)
                     /gene="ribB"
                     /locus_tag="J1Z28_004005"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBA7718781.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5748..5896)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6018..6317
                     /locus_tag="J1Z28_004006"
     CDS             6018..6317
                     /locus_tag="J1Z28_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HBA7718782.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPIAELQSPPAIPPVDKAE"
     gene            complement(6352..6552)
                     /gene="glgS"
                     /locus_tag="J1Z28_004007"
     CDS             complement(6352..6552)
                     /gene="glgS"
                     /locus_tag="J1Z28_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBA7718783.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
BASE COUNT         1893 a         1608 c         1472 g         1687 t
ORIGIN      
        1 ttgttatcag gcgttgcttt taacgctctt gctgacgatg ctaatcaggg ttcaggtaaa
       61 attactttta aaggtgaagt tatcgatgca ccttgttcta ttgctcctgg tgatgaagat
      121 cagacaataa acctcggtga agttgctgat accgtattaa aaagcggtca gaaatcactg
      181 cctgtagatg tcaccattca tttgcaggat tgtattttat ctgacggcac taacactgtt
      241 gataaagtca aaatcacctt tagttctgcc agtgttgacg ctaccgactc caacctgctt
      301 aaaaacactc tggaaggtaa catcggcggc gcaactgatg taggcgtacg tctggtgaaa
      361 tcagacaaca ccaacgtgac tcttggcact ccaatcacta tcaacttccc gacgactaac
      421 tcttaccagg agttgaactt taaagcccgt atggagtctc tgggacgcac cgcgaccccg
      481 ggtaacgtgc aggcacaggc taattacgta ctcgactaca agtaatcggt agtcaatacc
      541 tacacaacgc caggcggtaa agccgatgaa atttcatcgg caactttggg cctttttaga
      601 aatggatttt tatgaacaat aaaaacacgt tttcccggga taagttatcc catgcaatta
      661 aaaatgccct gtctggcgtt gtgtgttccc tactcttcgt tttgccagtc cacgccgtag
      721 aattcaacgt cgatatgatt gacgcagaag accgtgagaa tatcgacatc tctcgttttg
      781 agaaaaaagg ctatatcccc cctggtagat acctcgttcg tgtgcaaata aataaaaata
      841 tgttgccaca aacgttaata ctggaatggg taaaagccga taatgaaagt ggttcgttac
      901 tctgcttaac caaagaaaat ttgactaatt tcggtcttaa tacggaattt attgaatcat
      961 tgcaaaacat agctggcagc gaatgtctcg atttaagcca acgtcaggag ttaacgacac
     1021 gacttgataa agctacgatg atattatcgc taagtgttcc ccaggcatgg ttaaaatacc
     1081 aggcaacaaa ctggacgcca ccagagtttt gggatgccgg tatcgccggg tttatccttg
     1141 attacaacgt gtacgccagc cagtatgccc cacatcacgg atacagcacc caaaacgtca
     1201 gctcctatgg tacgttaggc tttaacctcg gcgcatggcg cttacgtagc gattaccaat
     1261 ataatcagaa ttttgctgat ggacgctcgg taaaccgcga cagcgaattt gcgcgaactt
     1321 atctgtttcg ccctatcccc tcctggtcgt caaaattcac tatgggccag tacgacctga
     1381 gctccaatct ttacgatacc ttccacttta ctggcgcatc gctggaaagt gatgaaagca
     1441 tgctgccgcc agatttacag ggttatgcgc cacaaattac cggcatcgcg cagaccaacg
     1501 cgaaagtaac tgtggcacaa aatggtcgtg tactttatca aaccactgtc gcgccaggcc
     1561 cttttactat ttctgatttg gggcaatcgt ttcaggggca gctggatgtc acagtggaag
     1621 aagaagatgg ccgcaccagc accttccagg ttggctccgc atccattccc tatttaaccc
     1681 gtaaagggca agtgcgctat aaaacgtcac tgggaaaacc gacatccgtc gggcataacg
     1741 atatcaataa tccctttttc tggacggcgg aagcctcctg gggctggctg aacaatgtgt
     1801 cgttgtatgg tggtggcatg ttcaccgctg atgattatca ggctatcact accggtattg
     1861 gctttaacct taaccaattc ggttcgcttt cttttgatgt caccggagca gacgcgtctt
     1921 tacagcaaca aaatagcggc aatctgcgtg gttacagcta tcgcttcaac tatgcaaagc
     1981 atttcgaatc gacaggcagt cagattacct tcgcgggtta tcgcttctca gataaagatt
     2041 acgtgtcgat gagtgagtac ctcagctcgc gtaatggcga tgagtcaatc gataatgaaa
     2101 aagagagtta tgtcatttcc ttgaaccagt actttgaaac gctggaatta aactcttatc
     2161 tcaacgttac acgcaatact tattgggaca gcaccagcaa taccaactac tccgtatctg
     2221 taagcaaaaa ctttgatatt ggcgatttca aaggtatatc tgcatcgctg gcagtaagtc
     2281 gaatccgctg ggatgacgac gaagagaatc aatattactt ctctttctct ctacctttac
     2341 aacaaaaccg caacatctcc tacagtatgc agcgaacggg aagcagtaat acttcgcaga
     2401 tgatttcctg gtacgattca tcagatcgca acaatatctg gaatatttca gcgtcggcaa
     2461 cggacgacaa tatacgtgat ggcgaaccaa cactgcgcgg cagctaccag cactattcgc
     2521 cgtggggacg tctgaacatt aatggcagtg tacagccgaa tcagtacaat tctgttaccg
     2581 caggctggta cggttcactt accgctacac gtcatggtat cgcccttcac gattatagct
     2641 atggcgataa cgcccgcatg atggtcgata ccgatggcat ctccggcatt gaaatcaact
     2701 ctaaccgtac cgttaccaac gggctgggca tcgccgtgat accttcgtta tcgaactaca
     2761 ccacctccat gttgcgggtg aacaataacg atctgccaga aggtgtcgat gtcgaaaact
     2821 cggttattcg caccacgctc acccagggtg ccatcggcta cgcaaaactg aatgccacca
     2881 ccggttacca aatcgtcggc gttattcgtc aggaaaatgg gcgcttccct ccactgggtg
     2941 tgaatgtcac ggataaagcg acaggtaaag atgtgggcct ggtcgcggaa gacggcttcg
     3001 tttatctcag cggtattcag gaaaatagta ctctgcattt aacctggggt gataatacct
     3061 gtgaagtcac gccgccaaac caaagtaaca ttagcgaaag cgcaataatt ttaccttgta
     3121 aaacagtcaa ataattcagg atgaatataa tgaaatcacc attaatcaca acaggaatga
     3181 tttgtttact ggggatctgc aactttgccc aggcgactgt ctcccccgac agaacacgca
     3241 tcatttttaa tgcttcgaat aaaagcgcaa cggttcgcct gaccaaccaa agtaaaatcg
     3301 atccctatct ggctcagtcg tggattgaag atgccagcgg taaaaaaacg cgtgattaca
     3361 tctcgacatt gccgccgatg gaacgtattg agcctgatga acagatacaa attcgcctga
     3421 tggcactggc ctcgttaaac gacttaccac aggaccgaga aacgttgttc tactataacg
     3481 tgcgagaaat tccgccgcgc gccaaagaac agaatgtaat gcaaatcgcc atgcaaagcc
     3541 gcctgaagct attctggcgg ccaaaagcga ttgagttaaa agagggggag atgattcctc
     3601 tgcaaaaagt aacgattacc cgtaccgccg ccggtctgac cctgaataac ccgacgcctt
     3661 atcatattac cgtcggttat atcggcacta atggcaagac actgatgccc ggtgcggaca
     3721 gtattatggt ggcaccattt acttctgcaa ctcaacacct aagcagcctg ccctctacct
     3781 tccagttagg ctttgtggcg gactacggcg ggctggagat gtttaaagtc gaatgtaatt
     3841 ccatccaatc cctctgccaa agctctcctg ctaagaaggg gaaaatatga ttacgctttt
     3901 tcgtttactg gcgattcttt gcctgttttt taatgtttca gcttttgctg ttgattgcta
     3961 tcaggatggg tacagaggaa caaccctcat aaatggagat ttaccagcgt tcaaaattcc
     4021 agagaacgcg caacctgggc aaaaaatttg ggagagtgga gatattaata tcacggttta
     4081 ttgtgacaat gcaccaggat ggacaacaga caatccatca gaaaatatct atgcctggat
     4141 caaattaccc caaataaata gtgccgacat gttgaataat ccatatttaa cctttggcgt
     4201 gacttataat ggtgtagatt atgaagggat aaatgaaaga attgatactc atgcatgcct
     4261 ggataaatat gagcaatact ataatgggta ttattatgac cctgtatgca atggcagtac
     4321 ccttcaaaaa aatgtaacat ttaatgccca ttttcgcgtc tatgtaaaat tcaaaagccg
     4381 cccagcagga gatcagacgg tgaactttgg catagtcaac gtgctacaat tcgatggcga
     4441 aggaggggca aacatgtcct ctaacgcgaa gaatttacgc tatgtgatta cgggattaga
     4501 taatatttca ttccttgact gtagtgtcga cgtccggatt tttccagaaa gccagatagt
     4561 taatttcggg caaattgctg caaactctat tgctacttat cgaccaaaag ctgcatttag
     4621 cgtatctact ataaaagatg ttgctgccga ttgtaccgag caatttgatg ttgccaccag
     4681 cttttatacg acagatacgt tacatgatga tacgcatctg gaaatgggta atggtttgtt
     4741 gatgcgtatt actgatcaaa aaacacaaga agacattaag tttaatcagt acaaacgctt
     4801 caccacatat attccaggtc agactggagc gatagttacc cgtgattacc aggctgaact
     4861 ttcacaaaaa ccgggggaga ctctcgttta tgggcctttc aaaaaagatc tcatcgtaaa
     4921 aataaactat aactgaacag caaaatgccg ggaacaaccc cggcattttc atatttagta
     4981 tttcacgtca ttagctggct ttacgctcat gtgcctgacg gtatgccacc agatcttcaa
     5041 tagtcacgag cgccatattg tgtttattgg caaactcaat gcactctggt gcacgcgcca
     5101 tcgtgccatc gtcattagtc agctcacaca gcacaccagc tggtttaaag cctgccagcg
     5161 tcatcagatc gatagtcgct tcagtatgac cgccacgcgt cagtacacca cctgcctgcg
     5221 cgcgaagtgg gaaaacgtgg cctgggcgat tcagatctga aggttttgcg ccatcggcaa
     5281 tcgctgcgcg aacggtcgta atacggtcag cggcagaaac accggtagtc acaccttcag
     5341 ctgcttcaat ggtcacggta aaaccggtgc cataggcgct ggtgttattt tctaccatca
     5401 ttggcagatc gagttgttta cggcgatcgt cagtaatgca caggcaaaca ataccactcc
     5461 cgtggcgaat ggtcagcgcc atctgctcaa cggtcatggt ttctgccggg aagatcatat
     5521 cgccttcgtt ttcacggtct tcatcatcaa gcaccatcac accgcgtcct tcacgcagcg
     5581 cagccagtgc attttcaaca cgttcgaaag gcgtaccaaa agaggaaagt agcgtctgat
     5641 tcatggtaaa aaaacctcac taaaattatg gttaccagaa tcagggcagt cttaggagtg
     5701 gcggcatata gccaaaataa cgtgagcggg tccatgcccg acagaatcgt tactctctcc
     5761 catccggact ctaaccgtcg gccccggaat tacaccggat ctgctgtcct ttgagttcgc
     5821 acccaaagcg ctcgcgggct ttcaactgag ttgatttacc gccggtgggg aatttcgccc
     5881 cgccctgaga ataagcggat tcactataac gctaatgatt agcgccagca acgcatagct
     5941 tcacataatt ctggtttatg acttaccctt atcgcactac aatggcactc aacacctatc
     6001 agtacaggga aactacaatg attgacccga aaaaaattga gcaaatcgct cgccaggttc
     6061 acgaatcaat gcctaaagga atcagggagt tcggggaaga tgtggagaaa aaaatccgcc
     6121 aaaccctaca agcgcagctg acgcgtctcg atctggtaag ccgcgaagaa ttcgacgttc
     6181 agacgcaagt cctgttacgg actcgtgaaa aactggcgtt actagagcag cgcatcagcg
     6241 aactggaagc gcgcaataat ccgatagcag aacttcaatc tcctccggct atcccgccag
     6301 ttgataaagc agaataaagc accacaaaaa cgggccagac ggcccgtaat atcagtgttc
     6361 taattcaagt tctctggttt gcatcatctg ttggcaatac caggcataac gcgcgcaaaa
     6421 ccaggttcta tgttcttcat ccatgttacc agtaacggcc agaatatcga ccgggcgacc
     6481 ttctgcgtgc attctggcaa aactacgcgc gagaaaatcg aaattattta aagaattaag
     6541 actatgatcc ataagcacat cctccggtta ttgcatacct tttaaggcca tatgcttttt
     6601 ctgatttcag tattccccaa tcagtgtagt gcctttaaac ataacgtgcg taaatatatt
//
LOCUS       DADSZD010000207         6606 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.209, whole genome shotgun sequence.
ACCESSION   DADSZD010000207 DADSZD010000000
VERSION     DADSZD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.209"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..544)
                     /locus_tag="J1Z28_004008"
     CDS             complement(<1..544)
                     /locus_tag="J1Z28_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HBA7718784.1"
                     /translation="MSKIFICVAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDSAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDVASASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDIATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKP"
     gene            complement(640..828)
                     /locus_tag="J1Z28_004009"
     CDS             complement(640..828)
                     /locus_tag="J1Z28_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HBA7718785.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            complement(825..1013)
                     /locus_tag="J1Z28_004010"
     CDS             complement(825..1013)
                     /locus_tag="J1Z28_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor"
                     /protein_id="HBA7718786.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     AILSRIALIKLGIFGERQ"
     gene            complement(1729..2100)
                     /locus_tag="J1Z28_004011"
     CDS             complement(1729..2100)
                     /locus_tag="J1Z28_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718787.1"
                     /translation="MRLTMEFKYLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMAR
                     NVRDAFTGLFSDTALNIHGTEDTAKQFAEKEVEDLSSKSQKQPNNHEINELLLATGFI
                     NINEYERRKKMLSDQSIKISF"
     gene            complement(2100..2252)
                     /locus_tag="J1Z28_004012"
     CDS             complement(2100..2252)
                     /locus_tag="J1Z28_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 domain-containing protein"
                     /protein_id="HBA7718788.1"
                     /translation="MDTLNLSNNESLVCGVFPNQDGTFTAMTYTKSKTFKTAAGAHRW
                     LARNTD"
     gene            complement(2573..3049)
                     /locus_tag="J1Z28_004013"
     CDS             complement(2573..3049)
                     /locus_tag="J1Z28_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7718789.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGSVKSKAEVARHFKVQPPSIYDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTSLISDTKSELLDINSLYQAASD
                     EKRAIVAFLLSENATEPGWVDHDVRAYIATMEMKVGKYLKDQESERKSQNITKTGT"
     gene            3175..3498
                     /locus_tag="J1Z28_004014"
     CDS             3175..3498
                     /locus_tag="J1Z28_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7718790.1"
                     /translation="MKKENYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATRGGVLCEELRPDVDWSYLRRSSCYSLNMLMKQPNDENEHTRNIKRQMI
                     HENQP"
     gene            3482..3907
                     /locus_tag="J1Z28_004015"
     CDS             3482..3907
                     /locus_tag="J1Z28_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBA7718791.1"
                     /translation="MKISHEHIRMAMNAWAHPDGEKVPTAKITKAYFELGMTFPELYG
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVVMH"
     gene            complement(4142..4537)
                     /locus_tag="J1Z28_004016"
     CDS             complement(4142..4537)
                     /locus_tag="J1Z28_004016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718792.1"
                     /translation="MDFATITSSVVLSACVAGVVSLVNGAWQRKSERTIEAQRRAAEA
                     RAKIREMALTLALKEWELHQTISKAKGYGVSGPEVYVFRYFRMLNLMEEDKFTIENLR
                     LIQYDSMCAVAAIQAEIERYREKNGLPMP"
     gene            4617..5702
                     /locus_tag="J1Z28_004017"
     CDS             4617..5702
                     /locus_tag="J1Z28_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBA7718793.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGPEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSAFYDRYKTDFHLDVRRNNRRNSARGFEEALKGLLSQ
                     EQEQEKEQDQDKNTMLHGEKIATNQAGDVQNVNPGQPAGTTPEADSGAVQQAMTARPE
                     QSHQLQQPEADSAIQREADQVVPENTGQPVRRVDYPDVFEQVWREYPLRAGANPKKSA
                     FSAWKARLREGVPPEAMLDGVRRYARYLATTGKTGTEFVQRATTFFGPDRNFENPWLL
                     PVSGTNNQRCVNHVSEPDTEIPPGFRG"
     gene            5743..6168
                     /locus_tag="J1Z28_004018"
     CDS             5743..6168
                     /locus_tag="J1Z28_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBA7718794.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKKTPVVDPDLIWSLPD
                     GEIRRYDRRQNIICSECRKSEVMQRVLVFYQGNFQEVVL"
     gene            6169..6582
                     /locus_tag="J1Z28_004019"
     CDS             6169..6582
                     /locus_tag="J1Z28_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718795.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASVRVDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRL
                     LRQEEQERRKNIEKLIQIERNAKARISRVTKSRTE"
BASE COUNT         1721 a         1442 c         1669 g         1774 t
ORIGIN      
        1 caggcttgca tttatgctta acccacccga tggcatgcag cttaagctcc ggatacatgg
       61 cgttaacttc tggcattttc atcaacgctt caacgatatg gccgtcgaat gttgcaatgt
      121 cttcctgcaa caattcctgt gcgctaatca ccatatcaac agtgatgttt tcacatgtgt
      181 cgaacttaac catgacggcg ttctgtactt caggggccag cttgtcaaag gtgacgttca
      241 tcggatcgga ttcagtctcg accgggacaa aggaagcaga cgccacatcc cagcggtttt
      301 cctgcatata ttcggtatcc caggaatcga gggcagggcg gggtatgccg ggtttatcct
      361 cacagacaat aaatttataa gcgctgtcct gagctgccgg gaattgctcc agaaattgcc
      421 agtgaaattt tgcgcgtgcg cgacgctcat caccggcttc aatggcagtg gccaccgcaa
      481 cagcgctatc ttcttttatg gcctgttcat caggaatagc cacgcaaata aagattttac
      541 tcattgtgtt ttaacctcat tacagatttc agggtgaacg aatccctgcc attgctggca
      601 tttttaatcc gttggtatgg cgttaatatg gctggagggt tatccagccg gtgtttcgtt
      661 attcaggtac agcgatactt tttttaccgg gaggcattca ccagaaattt tttgctcgtc
      721 tcttgcctgg aggcaggatt ctttactggc ataaattccg gtaatcacat tctgtgattc
      781 acccgttata agaaaaaccg tcatcaccag tgcaaatgct gaagtcattg acgttctccg
      841 aaaataccaa gtttaataag ggcaattcgg gaaagaatgg cattatcatt gagcagataa
      901 ggctcatatt tccgcatatt aatggcatcc tcagtaaacg ctttattact gagcagaaca
      961 ccaatatcaa aacaaccttc agacgtatta acgtttggta ataacgtttc cattatcgcg
     1021 tcctcaacaa tgaattttgt gatgccgtgc ctggtgcctc caggtgacgt taaccagtta
     1081 acaattaacg ccggattgtt agttgatgtc tgttacgcaa gttaaagacc gcttgtttta
     1141 actgttccgc gtgcgcatgg ccgcattcac cgcatcacaa aattcacttt aaaaagagcg
     1201 gatatccatt tccgccgaat caccagaaaa gtgataacag aggttgttgt ggctggggtg
     1261 tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacgggggaa ggaataaccc
     1321 cgccatactt accgccgcgc tatttcgcgg attgccacaa ccggaaacgc acggttgaag
     1381 aaatttaacg acaagcctta tatgcaaagg aatctcgccg tgcgctttcg tgttatgccc
     1441 tgacttttca gggatatatc ctttcagtaa actgtcagtg ccggattctt atccgtgtcc
     1501 ggcgcacgac cacacgctgt cacgagaggt ctccattctc aaccagtaac ctcaatggag
     1561 gataaaatgt cagagcagga gttaaaactt ggtgcatgtt attgtgttct caaggagctg
     1621 gtacacatgc ttccatctac tcagtatcaa cagttagttg gcaatttaaa tcagcgaatc
     1681 gaagctatgt taaaatctga tggttttaat aacgtagaaa cgctgatgct aaaaagatat
     1741 cttgatggat tgatcagata acattttttt acggcgttca tattcgttaa tatttatgaa
     1801 gcctgttgcc agaagcagtt cgttaatttc atggttgttt ggctgtttct ggctctttga
     1861 actgagatct tccacttctt tttctgcaaa ttgttttgct gtatcctctg tgccatggat
     1921 atttaaagct gtatctgaaa acagcccagt aaacgcatcg cgcacattac gagccatatt
     1981 atcagtgtct ttttttgtta ccgattccaa ttcaagttcg ttcagacgat gacgaagtgt
     2041 gtgtgctgca atctcctgga ttgaaggcgg taaatattta aattccatcg tcaacctcat
     2101 cagtcagtgt ttcttgctaa ccagcgatgc gcgccagctg cggttttaaa cgttttactt
     2161 ttggtatacg tcatcgcggt gaaggtgccg tcctggttag ggaacacacc gcataccaga
     2221 gattcgttgt tgctaagatt gagcgtatcc atgttgacct catttcccct taacgccggg
     2281 gtagcggaac taaaaacctg ctgcgctgtt atacaaagtg ttcccgccgt catgttcata
     2341 cgcctcgggc tggctactta acccctaatc actgccgggt aactcgaagt attgcccggt
     2401 gttctgtggg cggggtgggt tggtatggga gaactatagg taatgcctaa ttaattgtca
     2461 ataggctgcg cctaatgttt ttgatgcaaa cctaataggc gatgatatgt ggaagaggtg
     2521 atgggggggc tagataacgg gctctaggaa gttcccatca gaccatataa gtttaagttc
     2581 ccgttttggt gatgttctgg cttttccgtt ctgattcttg atctttcaaa tacttaccta
     2641 ccttcatctc cattgtggca atgtaggcgc gaacgtcatg atcaacccaa ccaggttccg
     2701 tggcattttc tgataagaga aaagccacaa tggctctttt ttcatcagag gcggcttgat
     2761 aaaggctgtt tatgtctaaa agttcacttt ttgtatctga aatgagggag gttggtatgg
     2821 ggtattcgtt aagcccccaa tgctctgggc caaccacatc agaaaagaaa cgccatagtt
     2881 ctggaagttt atctttactt atcgaacctt tcttaatcca atcatagatt gatggtggtt
     2941 ggactttgaa gtgacgcgct acctccgcct ttgatttgac ggatcctgat gcaatttttt
     3001 tgttaatggc ctgctctatc gctcggccta agtctttacc actaagcatt gcttaatact
     3061 ctcctatgct cattgaatta ggcaatacct acttcatttg cgttaggcga cgcctattga
     3121 catttgcatt aggcgtcgcc taatattgtt tcgtgttttt ttggagttca ttcgatgaaa
     3181 aaagagaact attcattcaa acgagcgtgt gctgttgtcg gtgggcaatc tgcaatggct
     3241 aggcttttag gtgtatcacc tccaagcgta aatcaatgga tcaaaggtgt acgtcagttg
     3301 cctgctgagc gatgtccagc aattgaacgt gcaacaagag gtggtgttct gtgcgaagaa
     3361 cttcgtcctg atgttgactg gtcatactta cgacgctcgt catgctattc gctgaatatg
     3421 ttgatgaagc aaccaaatga cgaaaatgag catacccgaa atatcaagag gcaaatgatt
     3481 catgaaaatc agccatgagc acatccgcat ggcgatgaat gcctgggcgc atccggatgg
     3541 cgagaaagta ccaactgcga agattaccaa agcgtatttt gaactgggaa tgacatttcc
     3601 ggaactgtac ggcgacagcc atccggaagc cctagctcgt aatacccaga aaattttccg
     3661 ctgggtagag aaagacaccc ctgatgctgt tgaaaaaatt caggcgttgt taccagcgat
     3721 cgaaaaggca atgccacctt tgctggtggc ccgaatgcgc agccacagtt cagcctattt
     3781 tcgggagctg gtggagacgc gggaacgact ggtgagagac gctgatgatt ttgtcgcagt
     3841 ggcgattgcc ggtttcaatc agatgaaccg tggtggcccg gcaggaaatg ccgtggtgat
     3901 gcactaaaag cacggatggc gttaacaatg acggaggggg ctgtgaatct acctgatttt
     3961 tttactgttc ttgcctgtgg tattggtggg gctctggccg gacattttat cgtgaatctg
     4021 ttcacctggt atctctttgg tttcagggat tacttcaccc gatggacttt aaattgtctt
     4081 cgtcggttca ttgggcgcaa gcctgatatg aggatttgga aagatgaaaa atgctgattg
     4141 tttatggcat cgggaggccg tttttctccc gatatcgctc gatctctgcc tgaattgctg
     4201 caacagcaca catggagtcg tactggatca gtctgagatt ttcaatcgtg aatttgtctt
     4261 cctccatcag gttgagcata cgaaagtatc gaaagacgta aacttctggg ccgctgacac
     4321 catacccctt tgccttcgat atggtttggt ggagctccca ttctttcaat gcgagagtga
     4381 gagccatttc tcttattttt gctctggctt cagcagcccg gcgttgcgct tcaatggttc
     4441 gttccgattt gcgttgccag gcaccattaa ccagcgaaac cacgccagca acacacgcac
     4501 tcaacacaac gctacttgtg atagttgcaa aatccatgtc gaacctcctc tggttctgtt
     4561 gattggtgaa tcacagatta taaccagagg aaggtttggc tccagacgag gtgaacatgc
     4621 gtgattatgc aaaagtttcc ccgcgattct ggctgggaga aacggggaga gaacttagaa
     4681 aggcgggtgc agaagcgcaa gttgttgctt tttacctgat gacatcccct cacgcaaata
     4741 tgttgggttt gtattacctg ccagttttgt accttgctca tgaaaccggg cttggtccgg
     4801 aaggggcttc gaaggggctt aaaagggctg ttgaagctgg tttttgtagc tatgaccatg
     4861 atgccgagat ggtctgggtc catgaaatgg cagcctggca ggttggggaa acgttgaagc
     4921 ctggagataa ccgttgtgca ggtgtcagga atgagtatgc atcattacct gaaaacgcct
     4981 ttctgtcagc cttttacgac agatataaaa cggatttcca tctggatgtg aggcggaata
     5041 atcgccggaa ttcagcaagg ggcttcgaag aggctttaaa ggggcttcta agccaagaac
     5101 aggaacagga gaaagaacag gatcaggata aaaatactat gcttcatggc gaaaaaatcg
     5161 ccacaaacca ggcaggggat gttcagaacg tcaatcctgg tcagccagca ggcacgacac
     5221 cggaagccga ttcgggcgct gtgcagcagg cgatgaccgc aaggccggaa caatcacacc
     5281 aactgcagca gcctgaagcc gattccgcca ttcagcggga agccgatcag gtagtcccgg
     5341 aaaacaccgg gcagcctgtg agacgagtgg attatccgga tgtgttcgaa caggtctggc
     5401 gggaataccc gttgcgtgcc ggggcaaacc cgaagaaatc cgctttcagt gcctggaagg
     5461 ccagattacg cgagggggtg ccaccagagg ccatgctgga tggcgtgagg cgttacgcaa
     5521 gatacctggc gactaccggg aaaacgggaa cggaatttgt tcagcgagcg acgacgtttt
     5581 ttggaccgga ccggaatttt gaaaacccct ggttgctccc ggtaagcggc acgaacaacc
     5641 agcgttgtgt gaatcatgtt tctgaaccgg ataccgaaat tccgccaggc ttcagggggt
     5701 aagtgtttat ttcaggtcat gaggtaattt tcaggagggc ttgtggcaaa agtttttaca
     5761 caagaagagc gagaaaaaat taaagggcag gttgttgagc tagtacgccg gagtgggcgc
     5821 gagacgttac ggcaactgga agccaagaca ggtgcgacaa gatatctgat gagcgttctc
     5881 gccagagagc tggttgccag tggtgatgta tacaactctg gttacgggtt attcccgtct
     5941 gaacaggctc gtaaggactg gcaaaacgcc cgcaaaaaac tctcaagggc aaagctgaag
     6001 aaaaaaacac ctgtggttga tccggacctt atctggtcgt taccagacgg agaaatacgt
     6061 cgttatgaca ggcgtcagaa cataatctgt agcgagtgcc ggaagagcga agttatgcag
     6121 cgcgtgctgg tattttatca ggggaatttt caggaggtgg tgctgtgagt gaaattagct
     6181 atcaggcttc aattgccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca
     6241 gaagatgagt taagtaatat tgttttattt ccggtaaaag aggatgaccc acgtaatcag
     6301 gttaattttc tttatgagcc atcggaaaga ccatattgtc atcacgcctc tgtccgggtt
     6361 gacgaaaaag agcgtcaggt ccgctgtaaa atctgcggtg cagttgtgga gccatttgac
     6421 tggatgctct ctgtggcgaa aagagaaacc agactggcag atgatgtaag gctattgcgt
     6481 caggaggaac aggaaaggcg aaaaaatata gaaaagctaa ttcagattga gcgtaacgcg
     6541 aaagcgcgga taagcagggt gacaaaatca agaactgaat aatttaaatt tagcactgtt
     6601 aaaaat
//
LOCUS       DADSZD010000208         6584 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.210, whole genome shotgun sequence.
ACCESSION   DADSZD010000208 DADSZD010000000
VERSION     DADSZD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.210"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..164
                     /locus_tag="J1Z28_004020"
     CDS             <1..164
                     /locus_tag="J1Z28_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718796.1"
                     /translation="LITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETRHHT
                     LKENIAPRG"
     gene            391..1656
                     /locus_tag="J1Z28_004021"
     CDS             391..1656
                     /locus_tag="J1Z28_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718797.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            1668..2534
                     /gene="ydiB"
                     /locus_tag="J1Z28_004022"
     CDS             1668..2534
                     /gene="ydiB"
                     /locus_tag="J1Z28_004022"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBA7718798.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DKDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2565..3323
                     /gene="aroD"
                     /locus_tag="J1Z28_004023"
     CDS             2565..3323
                     /gene="aroD"
                     /locus_tag="J1Z28_004023"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBA7718799.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            3468..5063
                     /locus_tag="J1Z28_004024"
     CDS             3468..5063
                     /locus_tag="J1Z28_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HBA7718800.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            5077..6228
                     /locus_tag="J1Z28_004025"
     CDS             5077..6228
                     /locus_tag="J1Z28_004025"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBA7718801.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(6271..>6584)
                     /locus_tag="J1Z28_004026"
     CDS             complement(6271..>6584)
                     /locus_tag="J1Z28_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7718802.1"
                     /translation="QLSKKFRASVSYICHEFTKEYRISPINYVIQRRMTEAKWSLTNT
                     ELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILSEFPQPVSLA
                     G"
BASE COUNT         1748 a         1487 c         1629 g         1720 t
ORIGIN      
        1 cgttgattac ggctcatctt tcgcaaagaa gtattgccga tattatgtgg ttcgataccg
       61 ccatcgctgc tatcggcttt atactggcac tgtttattgg cttacgcagt cgcaaagaaa
      121 cgcggcatca cacgctaaag gaaaatatcg ctccgcgtgg gtaatgcaat attcttttca
      181 ggttatgcaa gatcttacgg ataaataact ctttctgcgc taactaagga aaatcgcgtt
      241 caaaaacaaa ctatgacatg caatattcct ggaaacataa actttatgcc atgtacccag
      301 ggaaaatcat cttcagtata gtaattatgt aaaccgccgg agagcaatac gtacggtaac
      361 gaaattatct ttcagcaagg agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca
      421 tttatcctgg ctgttctttg tatttacttc agctatttcc tgcacggcat tagtgttatt
      481 acgcttgccc aaaacatgac atctttggca gaaaagtttt ccactgacaa cgcgggcatt
      541 gcctatttaa tttccggtat cggattgggg cgattgatca gtattttatt cttcggagtg
      601 atctccgata agtttggtcg tcgggcggtg atattaatgg cagtaataat gtatctgcta
      661 ttcttctttg gtattcctgc ttgcccgaat ttaactctcg cctactgtct ggcagtgtgc
      721 gtaggtatcg ctaactcagc gctggatacg ggtggctacc ccgcgctcat ggaatgcttt
      781 ccgaaagcct ctggttcggc ggtcatactg gttaaagcga tggtgtcatt cgggcaaatg
      841 ttctacccaa tgctggtgag ctatatgttg ctcaataaca tctggtacgg ctatgggctg
      901 attattccgg gtattctgtt tgtactgatc acgctgatgc tgttgaaaag caaattcccc
      961 agccagttgg tggacgccag cgtagctaat gaattaccgc aaatgaacag caaaccgtta
     1021 gtctggctgg aaggtgtttc atcagtactg ttcggtgtag ccgcattctc gaccttttat
     1081 gtgattgtgg tgtggatgcc caaatatgcg atggcttttg ctggtatgtc agaagctgag
     1141 gcattaaaaa ccatctctta ttacagtatg ggttcgctgg tctgtgtctt tatttttgcc
     1201 gcactactga aaaaaatggt ccgtcccatc tgggctaatg tatttaactc tgcactggca
     1261 acaataacag ctgccattat ctacctgtac ccttctccat tggtgtgtaa tgccggagcc
     1321 tttgttatcg gtttctcagc agctggcggc attttacagc tcggcgtttc ggtcatgtca
     1381 gagtttttcc ccaaaagcaa agccaaagtc accagtatat atatgatgat gggtggactg
     1441 gctaactttg ttattccgct gattaccggt tatctgtcga acatcggcct gcaatatatc
     1501 attgttctcg attttacttt cgcgctgctg gccctgatta ccgcaattat tgtttttatc
     1561 cgctattacc gcgttttcat tattcctgaa aatgatgtgc ggtttggcga gcataaattt
     1621 agcacccggt taaacacaat taagcataga ggttaaagga gttaattatg gatgttaccg
     1681 caaaatacga attgattggg ttgatggcct atcccatccg ccacagttta tcgcccgaaa
     1741 tgcagaataa agccttagaa aaagcgggat tgccatttac ctatatggcc tttgaagtgg
     1801 ataaagatag ttttcccgga gcaattgaag gattaaaagc cctcaaaatg cgcggaactg
     1861 gtgtatcaat gccgaacaaa caactggcgt gtgaatatgt tgatgaatta acgccagcgg
     1921 ccaaactggt gggagccatc aacaccatcg ttaatgatga tggctatctg cgtggctata
     1981 acaccgacgg cacgggtcat attcgcgcca ttaaagagag cggttttgat atcaaaggca
     2041 aaacgatggt gctattaggg gccggtggtg cctcaacggc aattggcgcg cagggggcaa
     2101 ttgaaggttt aaaagaaatt aaactcttta accgtcggga tgagttcttc gataaagctc
     2161 tcgccttcgc gcagcgggtt aacgaaaaca ccgattgcgt cgtgacagtc accgatctcg
     2221 ccgatcagca agcctttgca gaagccctgg cttccgccga cattttaacc aatggcacca
     2281 aagtgggtat gaaacccctt gagaatgaat cattggttaa tgatatcagt ctgttacatc
     2341 cgggacttct ggtcactgaa tgcgtgtata acccgcatat gacgaagtta ttgcagcagg
     2401 cgcaacaagc gggttgcaaa acgattgatg gatacggcat gttgttgtgg caaggggctg
     2461 aacagttcac gttatggact ggcaaagatt tccctctgga atatgttaaa caggtcatgg
     2521 ggttcggtgc ctgacaggct gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg
     2581 taaaagatct cgtcattggt acgggcgcac ctaaaatcat cgtctcgctg atggcgaaag
     2641 atatcgccag cgtgaaatcc gaagctctcg cctatcgtga agcggacttt gatattctgg
     2701 aatggcgtgt ggaccactat gccgatctct ccaatgtgga gtctgtcata gcggcagcaa
     2761 aaattctccg tgaaaccatg ccagaaaaac cgctgctgtt taccttccgc agtgccaaag
     2821 aaggcggcga gcaggcgatt tccaccgagg cttatattgc actcaatcgt gcagccatcg
     2881 acagtggcct ggttgatatg atcgatctgg agttatttac cggcgatgat caggttaaag
     2941 aaaccgtcgc ctacgcccac gcgcatgatg tgaaagttgt catgtccaac catgacttcc
     3001 ataaaacgcc ggaagccgaa gaaatcattg cccgtctgcg caaaatgcag tccttcgacg
     3061 ccgatattcc taagattgcg ctgatgccgc aaagtaccag cgatgtgctg acgttgcttg
     3121 ccgcgaccct ggagatgcag gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg
     3181 caaaaactgg cgtaatttct cgtctggctg gtgaagtatt tgggtcggcg gcaacttttg
     3241 gtgcggtaaa aaaagcctct gcgccagggc aaatctcggt aaatgatttg cgtacggtat
     3301 taactatttt acatcaggca taagcaataa tatttcggcg ggaataccct cccccgccga
     3361 actaaaaaag tatttttaac cgtatttaat aaaaatattt cgtgagtctc tgtgcgctaa
     3421 ttctccatct ggcgtaggga aaatcacacc tgaatcagga attaacaatg aaacctgtaa
     3481 aaccacctcg tattaatgga cgagtgccgg tcctgtcggc acaggaagcg gtgaattata
     3541 ttcccgacga agcaacactt tgtgtgttag gcgctggcgg cggtattctg gaagccacca
     3601 cgttaattac tgctcttgct gataaatata aacagactca aacaccacgt aatttatcga
     3661 ttattagtcc aacagggctt ggcgatcgcg ccgaccgtgg tattagtccg ctggcgcaag
     3721 aaggtctggt gaaatgggca ttatgcggtc actggggaca atcgccgcgt atttctgaac
     3781 tcgcagaaca aaataaaatt attgcttata actatccaca aggtgtactt acacaaacct
     3841 tacgcgccgc cgcagcccac cagcctggta ttattagtga tattggcatc ggaacatttg
     3901 tcgatccacg ccagcaaggc ggcaaactga atgaagtcac taaagaagac ctgattaaac
     3961 tggtcgagtt tgataacaaa gaatatctct attacaaagc gatagcgcca gatatcgcct
     4021 tcattcgcgc taccacctgc gacagcgaag gctacgccac ttttgaagat gaggtgatgt
     4081 atctcgacgc attggttatt gcccaggcgg tgcacaataa cggaggtatt gtgatgatgc
     4141 aggtgcagaa aatggttaag aaagccacgc tgcatcctaa atctgtccgt attccgggtt
     4201 atctggtgga tattgtggtg gtcgatccgg atcaaaccca actgtatggc ggtgcgccgg
     4261 ttaaccgctt tatttctggt gacttcactc ttgatgacag caccaaactt agcctgcccc
     4321 taaaccaacg taaattagtt gcacggcgtg cattattcga aatgcgcaaa ggcgcagtgg
     4381 ggaatgtcgg cgtcggtatt gctgacggca ttggcctggt cgcccgggaa gaaggttgtg
     4441 ctgatgactt tattctgacg gtagaaacag gtccgattgg cggtattact tcacagggga
     4501 tcgcctttgg cgcgaacgtg aatacccgcg ccattctgga tatgacgtcc cagtttgatt
     4561 tttatcacgg tggtggtctg gatgtttgtt atttgagttt tgctgaagtc gaccagcacg
     4621 gtaacgtcgg cgtgcataaa ttcaatggta aaatcatggg caccggtgga tttattgata
     4681 tcagtgccac ttcgaagaaa atcgttttct gcggcacatt aactgcgggc agtttaaaaa
     4741 cagaaattac cgacggcaaa ttaaatatcg tccaggaagg acgggtgaag aaatttattc
     4801 gggaactacc ggaaataact ttcagcggaa aaatcgctct cgagcgaggg ctggatgttc
     4861 gttatatcac tgagcgcgcc gtattcacgc taaaagaaga cggcctgcat ttaatcgaaa
     4921 tcgctcctgg cgtcgattta caaaaagata ttctcgacaa aatggatttc accccagtga
     4981 tttcgccaga actcaaactg atggacgaaa gattatttat cgatgcggcg atgggttttg
     5041 tcctgcctga agcggctcat taacaggagt ataatgatgg atttctcttt aactgaagaa
     5101 caagaactgc tgctggccag tattcgcgaa ctgattacga ctaactttcc ggaagagtat
     5161 ttccgcacct gcgatcaaaa cgggacatac ccgcgtgagt ttatgcgggc actggcggat
     5221 aacggtattt ccatgcttgg cgtgccggaa gaatttggtg gtatccctgc ggattacgtc
     5281 acccaaatgc tggcgctgat ggaagtgtca aaatgcggtg ctccggcatt tttgattacc
     5341 aacggtcaat gtattcacag tatgcgccgt ttcggttctg cagagcagct acgtaaaacg
     5401 gcagagagca ccctggaaac gggtgatccc gcctatgccc tggcattgac agagccaggc
     5461 gcaggctcag acaacaacag tgccactacc acttacacgc gtaaaaacgg caaggtttac
     5521 atcaacggac agaaaacctt tattaccggt gcgaaagagt acccatatat gctggtgttg
     5581 gcgcgcgatc cgcaaccgaa agatccgaaa aaggccttta ctctgtggtg ggtcgactcc
     5641 agtaaacccg gcattaaaat caatccactg cataaaatcg gctggcatat gctcagcacc
     5701 tgcgaagtct atctcgacaa cgtggaagtt gaagagagcg acatggtggg cgaagaagga
     5761 atgggtttcc tcaatgtgat gtacaacttt gagatggagc gcctgatcaa cgccgcgcgc
     5821 agcaccggct ttgccgagtg tgcctttgaa gatgccgccc gctatgccaa ccagcgtatc
     5881 gcttttggta agccaattgg tcataaccag atgatccagg aaaaactggc gctgatggcg
     5941 attaagatcg acaacatgcg caacatggtg ttgaaagtgg catggcaagc cgatcagcat
     6001 cagtcactgc gcaccagcgc ggcgctggca aaactgtact gcgcacgtac cgcaatggaa
     6061 gtcattgatg atgcgattca aatcatgggc ggtctgggct ataccgatga ggcgcgcgtc
     6121 tcccgcttct ggcgtgatgt ccgttgtgaa cgtatcggcg gcggtacaga cgaaattatg
     6181 atttacgtag caggtcggca gatcctgaaa gattatcaga acaaataatc tgcaggcggc
     6241 gcagcttctt aacaaactgc gccgccagat ttatccggca agacttactg gttgaggaaa
     6301 ttcagatagg atttcttgtt ccgcaaaaca gtttttaaat tgcctgcggt aatcgctggg
     6361 cgaacagccg acatggcgca aaaacagttt ggcaaagtga tcgacatttt cataacccac
     6421 acgccaggag atctctgcct gtgataattc agtattagtg agtgaccatt tcgcttccgt
     6481 catacgccgt tgaataacat agttaatagg ggaaatacga tactctttgg taaattcatg
     6541 gcagatataa ctgacgctgg cacgaaattt tttcgataac tgct
//
LOCUS       DADSZD010000209         6501 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.211, whole genome shotgun sequence.
ACCESSION   DADSZD010000209 DADSZD010000000
VERSION     DADSZD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.211"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            198..4064
                     /gene="tssM"
                     /locus_tag="J1Z28_004027"
     CDS             198..4064
                     /gene="tssM"
                     /locus_tag="J1Z28_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBA7718803.1"
                     /translation="MQKFLSLLFSRRALAVVGVVMLALLVWFIGPLVAFDTLHPLASV
                     GSRVVTIVLLLMFLALWLVNWSMSIIGVTVLCLVIGFVSPLLAFGDVRPFAPLWLRLT
                     IIGMILLTYALYGLYQLWRALRLDEQLLRRFLHPRGEDVSVAGEIKADLRTVSHIVTR
                     AIRQLKQLRVDLPGWRKVLEGKRFLYELPWFMVIGSPGDGKTTALLNTGLQFPLAEQM
                     EQTAHILTVPGGGTLHCDWWFTNEAVLIDTAGRYARHDDGGEISATQRNAGEWQGFLG
                     LLRKHRPGAPLNGVILTLNVADLTAQSPAERLAACAALRARLAELRETLGIRFPVYLV
                     VTKMDLLPGFTEYFRGLTSHLRAQVWGFTLPYSRRRHASDPQSLHALCGQELANLTLR
                     LDQGLDTRLQEEYDLKSRQRLYRFPREFAALGAPLLEAIEQIFLDSKFDATQLNNTLR
                     GVFFTSAAQAQADAVADQTGIWQRFVRAIKGKVESPSHTLPDGNRSYFLHDLLTQFIF
                     REAHLVEPNLQWAWRYRLLRTGGHLLVLALAFWLWQGMQGSQQGNSGYLNEIASRTVQ
                     LDGDVKAYTGKPAMQPVPVLLNQARALSAWPGLDPDSPPLAWRYGLYSVPPVMESVAS
                     LYDRLLDQLLLPPLVKRMEYVLSDAIARQDSKAAYDALHVYLLLNLDKDHEDKYNAAE
                     IHAWVLNDLENRDSVAEFGGRAAVLEHIDALFDGSRVVHSPYDKDEGLIRMARAFLDG
                     HTSTERIYTRALASMESEAPQEFSLVRAVGADAGTVFVRTDGAPLDRGVPGIFTREGY
                     RELFDKLLSEFVASASADDAWVMGRENAQKKTTDIVMSQLPGQELPVTREVRRLYLAE
                     YARRWQDFLASIHSINSAGEDGSPGLAYDLQVLRTLASPDSPLMRLSKAVVEQTTLVP
                     PLDAQARQKQMAQRAQDRLSGNAGKAVQTAKLLQDIHPEERLEKSLVDDRFAALREVV
                     SGRTDSGQGGGAPQLNSLLTMLNEYYTQLTIADSALAASTLPGRISAADKLQLEAAKL
                     PAPLKNILLDLTQQGTRKINAGTGEVLNAQMEAVIGDECRDAIDGRYPFTDSPQEVSA
                     DDFNRIFASGGVLDAFWTKQLAPLADTASNPWRYKPTEGNMTLLGPDLTPFQEAKQIR
                     SVFFNGDGGKKFFWAMQVRVVDMDPAIAELVIDIDGQVLRYAHGPVRPLKVNWPGPRN
                     GSMAEITASPRIRQDTSTLLTNGPWALFHLLDAGNAQETAVRGTQQVEYNFDGRRVVL
                     EITAGRDFDPVNRGLLQRFACPERAL"
     gene            4311..5006
                     /locus_tag="J1Z28_004028"
     CDS             4311..5006
                     /locus_tag="J1Z28_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718804.1"
                     /translation="MRKYTRFFLIFLLYAMSGNVFADIRKNTGTIDPDYINLSAQEFL
                     SFYARGTSQEKENVLMYVLGVSDATESKTWCGYNKINEIEFYSSVLTYLNQQSVNDMN
                     MRASTLIEQAMVRLFPCPDVASQEKKSPRQLSVSHLTSGAFDLNGDDFFRFWQSDNRQ
                     DRLNAKIYLLGIEDATESKIWCDYKTIKTVTLDEIIYWHFRKKMSDKLNERAANIIIE
                     ELSKHPCDKGNNK"
     gene            5003..5479
                     /locus_tag="J1Z28_004029"
     CDS             5003..5479
                     /locus_tag="J1Z28_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718805.1"
                     /translation="MKPSFSTLKKNHYSSAQVDGDVLYSEVGYNYEALQQQNAGYVNT
                     CATRMSVALIKSGVRFNGRLTIKSGDWKGKKIETGAKLLADQLSRADVFGRPVIWRDS
                     EKFKTDTSNKRGVVFFWKITDYNGGHIDLIEPSTTGAICHSHCYFSCKEIWFWELN"
     gene            5668..6429
                     /locus_tag="J1Z28_004030"
     CDS             5668..6429
                     /locus_tag="J1Z28_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated
                     domain-containing protein"
                     /protein_id="HBA7718806.1"
                     /translation="MRRAEISAPAMFGRLPDQRDYVRWRVSANEGQVWQAWLNQQTWL
                     GQGRHIVLPEGTEVAKAGINDGWLHLSPREKTVQPEHHPLPWSFVLSPGFLPIGGRDE
                     WLTGVLTASRDSTGRPWPLVIYQRAGREWLEESLDDTQGWLYWLARLAAAQVAPQAER
                     RGRLAEQTDQLWAMWRPGPRWAQWLRGLRRVPQRSRELTNLPDTPVVELPGVRHLPWP
                     GWPEKMLAQQEQGWFWQQNSEGRYVDALRLAGENR"
BASE COUNT         1487 a         1616 c         1933 g         1465 t
ORIGIN      
        1 atgcaggata tggacccttc aataacagca ttacctcagg gaaacggaaa tagagatgta
       61 atcaggaaat taatttattc tatttcagtg gctattcaga ttgttcttta ttttctttca
      121 gtatcgcgta caggaaaggg attttcagcc ctgggtattt tgctattact gattaccgca
      181 tggagctctg aacaataatg caaaaatttc tcagtctgct gttttcccgc cgcgcgctgg
      241 ctgttgtcgg cgtggtgatg cttgcactgc tggtctggtt tatcgggccg ctggtggcgt
      301 ttgataccct gcatccgctg gcatctgtgg ggagccgggt agtcaccatc gttctgttac
      361 tgatgtttct cgcactgtgg ctggtgaact ggtcgatgag catcattggc gtgacggttc
      421 tgtgcctggt tatcggcttt gtctcaccgt tgctggcctt cggtgacgtt cgcccgtttg
      481 cgccgctttg gctgcgtctg acgattatcg gcatgatcct gctgacttat gcgctgtatg
      541 gactgtacca gctgtggcgg gcgctgcgtc tggatgaaca acttctgcgt cgtttcctgc
      601 atccgcgtgg ggaagacgta tcggtggcag gggaaattaa agccgactta cgcaccgtca
      661 gccatattgt tactcgcgct attcggcaac tgaaacaact gcgtgtggat ctgcctggct
      721 ggcgtaaggt cctggaagga aagcgttttc tgtacgagct gccgtggttt atggttatcg
      781 gtagccccgg cgatggtaaa accaccgcgc tacttaacac cgggctacag tttccgctgg
      841 cggagcagat ggagcagacc gcgcatatcc tcaccgtgcc gggcggcggc acgctgcact
      901 gcgactggtg gttcaccaac gaagcggtgc tcattgacac tgccgggcgc tatgcccgcc
      961 acgatgacgg cggtgaaatc agtgcgaccc agcgcaacgc cggggagtgg caggggtttc
     1021 tgggactgct gcgtaaacac cgccccggcg caccgctcaa cggcgtgatc ctgacgctga
     1081 acgtggcgga tctgactgcg cagtccccgg cggaacgact ggctgcctgt gcggccctgc
     1141 gtgcgcggct ggcggaactg cgggagacgc tgggtattcg tttcccggtt tatctggttg
     1201 tcaccaaaat ggatctgcta cctggattta cggagtattt ccgtgggcta accagtcacc
     1261 tgcgtgccca ggtctggggc ttcaccctgc cttacagccg caggcgtcac gccagcgatc
     1321 cgcagtcgtt gcatgcgctc tgtggccagg aactggcaaa tctgacgctg cggctggatc
     1381 aggggctgga tacccggttg caggaagagt acgaccttaa aagccgtcaa cggctctacc
     1441 gctttccgcg cgaatttgcc gcgctgggcg cgccgctgct ggaagccatt gaacagattt
     1501 tcctcgattc aaaatttgac gccactcagc ttaacaacac cctacgcggc gtatttttta
     1561 ccagcgccgc acaggcgcag gcagacgcgg tggccgatca gacgggcatc tggcagcgct
     1621 ttgtccgggc gatcaaaggg aaagtggagt caccttctca taccttaccg gacggcaacc
     1681 gcagctattt tcttcatgac ctactgaccc agtttatctt ccgtgaagca cacctggtgg
     1741 agccgaatct tcaatgggcc tggcgttatc gcctgctgcg tactggcggg catctgctgg
     1801 tactagcgtt ggctttctgg ttgtggcagg gaatgcaggg cagccagcag ggtaacagcg
     1861 gctatctgaa tgaaatcgcc agccggacgg tccaactgga tggtgacgtc aaagcctata
     1921 ccggaaaacc tgctatgcag cctgtcccag tcctgttgaa ccaggcgcgg gcgctctctg
     1981 cctggccggg gctggacccg gactcaccgc cgctggcctg gcgttatggt ctttacagcg
     2041 ttccgccagt aatggagagc gtcgcatccc tgtatgaccg tctgctggac cagctcctat
     2101 tgccgccact ggtgaagcgc atggaatatg tgctttcaga tgccattgct cgtcaggaca
     2161 gcaaagccgc gtatgacgct ctgcatgttt atctgctgct gaatcttgat aaagaccacg
     2221 aggacaaata caacgcagcg gagatccacg cctgggtact gaacgatctg gaaaaccgcg
     2281 atagcgtggc ggagttcggc ggccgtgcag cagtgctgga acatatcgac gccctgtttg
     2341 acggcagccg cgtggtgcat tcgccttatg acaaagatga gggactgatc cgcatggcgc
     2401 gggctttcct tgacggtcat accagcaccg agcgtatcta tactcgagcc ctggcttcca
     2461 tggagagcga agcaccgcag gagttttcgc tggttcgcgc ggtgggcgcg gatgcgggga
     2521 cggtttttgt ccgcacggat ggcgcgccac tggatagggg cgtgcccggc atctttactc
     2581 gcgaaggcta ccgggaactg ttcgacaaac tgctgtcgga gtttgtcgcc tccgcatcag
     2641 cggacgacgc ctgggtaatg ggccgggaaa acgcgcaaaa aaaaacgact gatattgtga
     2701 tgagccagtt gccggggcag gaactcccgg ttacccgtga ggtacgccgc ctgtatctgg
     2761 ctgaatatgc ccgccgctgg caggattttc tggctagtat tcacagcatc aacagtgccg
     2821 gggaagacgg tagtcccgga ctggcgtatg accttcaggt gcttcgcaca ctggcctcgc
     2881 ctgactcacc tctgatgcgc ctgagtaaag cggtggtgga gcagaccacg ctggtaccgc
     2941 cgctggatgc gcaggccaga caaaaacaaa tggcccagcg cgcgcaggac cggctctccg
     3001 gtaatgcggg taaagcagtg cagacggcaa agctgttgca ggatatccac ccggaagagc
     3061 ggctggaaaa atcgctggtg gatgaccgtt tcgccgctct gcgggaagtg gtttccgggc
     3121 ggacggacag tggacagggg ggcggagcgc cgcagttaaa ttcgctgctg acaatgctca
     3181 atgagtatta cacccagctc accatcgccg acagtgcatt ggcagcgagc acattaccgg
     3241 gccgcatttc ggcggcggat aaactacaac tggaagcggc aaaactgccc gcaccgctga
     3301 aaaatattct gctggatctc acgcagcagg gaacgcgcaa aatcaacgcc ggcaccggtg
     3361 aagtgctgaa cgcgcagatg gaagcggtga tcggtgatga gtgccgcgac gccatcgacg
     3421 ggcgctaccc cttcaccgac agcccgcagg aagtcagtgc ggacgatttt aaccgtatct
     3481 ttgccagcgg cggcgtgctg gatgccttct ggacgaagca actggcccct ctggcggata
     3541 ccgccagcaa cccgtggcgc tacaaaccga ccgaaggcaa catgacattg cttgggccgg
     3601 accttacacc gtttcaggag gcgaaacaga tccgcagcgt tttttttaac ggtgacggcg
     3661 ggaaaaagtt tttctgggcg atgcaggttc gcgtcgtgga tatggatcct gccatcgccg
     3721 aactggtgat tgacattgac ggccaggtgc tgcgttacgc ccacggtcct gtcaggccgc
     3781 taaaagtgaa ctggccgggg ccgcgcaacg gttcaatggc agaaatcact gccagcccgc
     3841 gcatccgcca ggacacctcg acgctactga ctaacgggcc atgggcgctg tttcacctgc
     3901 tggatgcggg taacgcgcag gagacggcgg tgcggggaac acagcaggtg gaatacaatt
     3961 ttgacgggcg gcgggtggtg ctggaaatca ccgccggacg agactttgat ccggtgaacc
     4021 gcgggctgct gcaacgcttc gcctgcccgg agcgggcgct gtgatgtgtc gggcggcggc
     4081 agtatcgttg gcagactgcc ggacccacgt gactatgcta aaggctatat tcacggatgt
     4141 cttgacgtgg agttgtatgc taaaacagac cgttgcacca ggtgagcaaa aaggcaccct
     4201 taatgtcact tatatactga gatggcaggt caatgacgca acaaaactat aagctttctt
     4261 aaaccgacgt tatagcgacc atcccactac cgtgattcag gaagcatgat atgcgaaaat
     4321 atacgcgttt ttttcttatt tttttgttat atgccatgtc aggaaatgtt tttgctgata
     4381 tcagaaaaaa cacaggaact attgaccccg attatattaa tctcagtgcg caggaatttt
     4441 tgtcatttta tgcccgtgga acgtcacagg aaaaagaaaa tgtgctgatg tatgtacttg
     4501 gagtgagtga tgcgacagaa agtaaaacct ggtgtggata taataagatt aatgaaatag
     4561 aattttattc atcggtactg acatatctta atcagcagtc ggttaacgat atgaatatgc
     4621 gagcttcaac tctgattgaa caagcgatgg tcaggctgtt tccctgcccg gatgtagcat
     4681 cacaggaaaa aaaatctccc cggcagctat ctgtttcaca cctgacctct ggcgcgttcg
     4741 atctgaacgg agatgatttt ttccgcttct ggcagtcaga taacaggcag gacagactta
     4801 atgcaaaaat ctatcttttg gggatagaag atgctacgga aagcaaaata tggtgtgatt
     4861 ataaaacgat taaaacagtt accctggatg aaatcattta ctggcatttc agaaaaaaaa
     4921 tgtcagataa actgaatgaa cgggctgcaa atattattat tgaggaacta agtaaacatc
     4981 cctgtgataa aggaaataat aaatgaaacc ttcattttcc acacttaaaa agaaccatta
     5041 ctcatcagct caggttgatg gtgatgttct gtacagtgaa gtgggttata actatgaagc
     5101 tctgcaacag caaaatgctg gttatgtaaa tacctgtgcc acacgtatga gtgtggcgct
     5161 gattaaatca ggcgtcagat ttaacgggcg actgacgatc aaaagtggcg actggaaagg
     5221 gaagaaaata gaaaccggtg cgaaattact ggcagatcag ctttcccggg ctgatgtttt
     5281 tggaagacca gtcatctggc gtgactcaga aaaatttaaa acagacacca gtaataaaag
     5341 aggagtggtc tttttctgga aaattacgga ttataacggt ggacatattg atcttattga
     5401 accttctacc acaggcgcta tatgtcattc acattgttat tttagctgca aggaaatctg
     5461 gttctgggaa ctgaattaac aatttcatcg gaagctgatg ccttttctct ttgtaggggg
     5521 gagacctgag cgattttatt aaaaatgctc tgattcctgt ggaagaaaat taatcctgat
     5581 atctgacttt atgattgaaa tgaagaatta tgttctgtat taccgggaac tgctgcaaaa
     5641 cttcacctgt ccggtcagga cgttgtgatg cgccgggcag agatatcggc cccggcgatg
     5701 tttggcaggc tgccggacca gcgggactat gtgcgctggc gggtgagtgc gaacgaggga
     5761 caggtgtggc aggcgtggct gaaccagcag acgtggctgg ggcagggacg gcatattgtt
     5821 ctgccggaag gaacggaagt tgcgaaggcc ggaataaatg acggttggtt acacctgtca
     5881 ccgcgcgaga aaacggtgca gccggaacac catccattgc cgtggagctt tgtcctgtcg
     5941 ccgggttttt tacccattgg tgggcgcgat gagtggctga ccggggtact gaccgcatcg
     6001 cgagacagca cgggcaggcc gtggccactg gtgatttacc agcgtgccgg gcgggaatgg
     6061 ctggaagaga gtctggatga cacacagggc tggctgtact ggctggcgcg gctggcggcg
     6121 gcgcaggtgg caccacaggc ggaacggcgc ggacgactgg cggagcagac agatcagctc
     6181 tgggcgatgt ggcgaccggg gccgcgctgg gcacagtggc tgcgcggctt gcgacgtgta
     6241 ccgcagcgca gccgggaact gaccaacctg ccggacacgc cggtagtgga attgcccggt
     6301 gtgcgccacc tgccgtggcc gggctggccg gagaaaatgc tggcgcagca ggagcagggc
     6361 tggttctggc agcagaacag cgaggggcgg tatgtggatg cgctacggct ggcgggggaa
     6421 aacaggtaaa ggattttata tgagtaaacg tgaagaaatt aaaggtaacc tgaagtttga
     6481 tgaactcaaa tttggcattg t
//
LOCUS       DADSZD010000210         6458 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.212, whole genome shotgun sequence.
ACCESSION   DADSZD010000210 DADSZD010000000
VERSION     DADSZD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.212"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            112..654
                     /locus_tag="J1Z28_004031"
     CDS             112..654
                     /locus_tag="J1Z28_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718807.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            754..1956
                     /locus_tag="J1Z28_004032"
     CDS             754..1956
                     /locus_tag="J1Z28_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBA7718808.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRADAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2176..2958
                     /locus_tag="J1Z28_004033"
     CDS             2176..2958
                     /locus_tag="J1Z28_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBA7718809.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPEAVQKSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2973..4183
                     /gene="rhmD"
                     /locus_tag="J1Z28_004034"
                     /pseudo
     CDS             2973..4183
                     /gene="rhmD"
                     /locus_tag="J1Z28_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            4240..5529
                     /locus_tag="J1Z28_004035"
     CDS             4240..5529
                     /locus_tag="J1Z28_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718810.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5547..6350
                     /gene="yfaU"
                     /locus_tag="J1Z28_004036"
     CDS             5547..6350
                     /gene="yfaU"
                     /locus_tag="J1Z28_004036"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBA7718811.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
BASE COUNT         1451 a         1564 c         1806 g         1637 t
ORIGIN      
        1 agttacaata agtgactaga tccctttttt cgctcattga atcctctgcg ccaggcagta
       61 caatcccgca gctttgggaa ttttaattaa ttgcttgtgg gaaaggggat aatgaaaaaa
      121 attgcgctag caggtctggc cggaatgctg ttggtttctg catcggtcaa tgcaatgagc
      181 atcagcggcc aggcgggtaa agaatacacc aatattggtg tcggttttgg gactgaatcg
      241 acgggcctgg cgttaagcgg taactggaca cataacgacg acgacggtga tgccgcgggc
      301 gtggggctgg ggttgaatct gcctctcgga ccattaatgg cgaccgttgg cggaaaaggc
      361 gtttacacca acccgaacga tggtgatgaa ggttatgcag cggcagttgg cggtggtttg
      421 cagtggcaaa ttggtgacag cttccgcttg tttggtgagt attactactc tccggattcg
      481 ctctccagcg gaattaaaag ttatgaagaa gcgaatgctg gcgcgcgtta caccattatg
      541 cgtccagtca gcattgaggc tggctaccgt tacctgaacc tggcaggtaa aaatggcaat
      601 cgtgataacg ccattgctga tggtccgtat gttggggtaa atgccagctt ctgacgcctt
      661 ccggcgcggc gttctgtcgc gccattcagt ttcttactac gccctgtctc cctttgcgtt
      721 atagtgattc gataacaaaa gcaggagata accatgttaa aagtggaaat gttatccacc
      781 ggggatgaag tgttacacgg gcaaatcgtt gacactaacg ctgcctggct ggccgatttt
      841 ttctttcatc aggggctgcc attatctcgc cgcaatacgg tgggggataa tcttgatgac
      901 ttagtcacca ttcttcgcga acgtagtcag cacgccgatg tgctgatcgt taacggcggg
      961 ctgggaccga ccagtgatga tttaagcgca ctcgccgctg cgacagcaaa aggtgaaggc
     1021 ctggtgctgc atgaagcctg gctcaaagag atggaacgct atttccatga acggggacga
     1081 gtaatggcac cgagcaaccg taaacaagcg gagctgcctg ccagtgctga atttatcaat
     1141 aacccggtag gcactgcctg tggttttgcc atacagctta atcgttgcct gatgttcttt
     1201 acccccggcg taccgtcaga atttaaggtg atggtcgagc acgaaatcct gccgcgcctg
     1261 cgcgagcgtt tttctttacc acagccgccg gtttgcctgc gtttgactac ctttggtcgt
     1321 tcggaaagcg atctggcaca aagcctggac actctacaac tgccgccggg cgtaacaatg
     1381 ggctatcgct cctcaatgcc gattatcgaa ctgaaactca ccggaccggc aagcgaggaa
     1441 caggcgatgg aaaaactgtg gctggacgtt aaacgtgttg ccggacagag cgtgattttc
     1501 gaaggcaccg aaggactgcc cgcgcagatc agtcgcgaat tgcagagccg ccagttcagc
     1561 ctgacgctga gcgagcaatt caccggtggt ttattggctt tgcaactttc tcgcgcagat
     1621 gctccattgc tggcgtgtga agtggttcct tcacaggagg aaaccctggc gcaaactgcg
     1681 cactggatta cagaacggcg ggcgaaccat tttgctgggt tggcactggc tgtttctggt
     1741 ctggaagatg agcatctcaa ctttgcacta gccacgccag acggcacttt cgctttgcgt
     1801 gtacatttca gtgccacacg ctacagtctg gctattcgcc aggaagtctg cgcaatgatg
     1861 gcgcttaata tgctacgccg ttggttaaac ggccaggaca tcgccagcga gcatggctgg
     1921 attgaggttg ttgagtccat gaccttatct gtctgattcc gccactctgt gccggtttac
     1981 tgcaccggca cattccccct ccggcaaaat ttgtccaacc tgttgtactc aataagaaaa
     2041 aatccgtgcg ctgattcaca aatttgtgcg taatttctcc agtccgctgg acgagaaacg
     2101 ggatttgatg cgcttcacaa ttttactccc cctgagcaac gagactgatt ccagtatcta
     2161 agactggagt ccagtatgtt ggaatcaagc aaagttcccg ccctgacacg ggctattgat
     2221 attctcaatt tgattgcccg gattggcccc tgtagtgctg cgacgatcat tgacacattg
     2281 gggattccta aaagcacagc ctatttgctg cttaatgaac tcaggcgtca gcgttttctt
     2341 agccttgatc accaggaaaa tttttgtttg tggaccaggc tggtggagct ttccggtcat
     2401 gcgttaagca agatggatct ccgggaactg gctcgaccgc gtttgacgca actgatggat
     2461 accaccgggc tgttatgtca cctggggatt attgataacg gcagcgctta ttacattttg
     2521 aaagtggagt catcagccac tatcagcgtg cgttcccatg aaggaaaaag cctttcgctt
     2581 tatcgctccg gaataggtaa atgcctgctt gcctggcaac ctgaagctgt acagaaaagt
     2641 attatcgaag agctggtctg ggagcagatg acacccacca ccattaccca tccacaacgg
     2701 ctctacgagg aactggcgcg tattcgccgt cagggctgga gctatgacaa cggcgaagat
     2761 tatgccgatg tccgttgtgt cgctgcgccg gtgtttaacg ccaataacga gcttaccgct
     2821 gcgatttcgg tggtcggtac ccgcttacaa atcaatgaag aataccgtga ttatctggcg
     2881 ggcaaggcca ttgcctgcgc cagggatatt tcacgtttgt tgggatggaa aagtcccttt
     2941 gacttacagg cctcataaca atggagaaca tcatgaccct accaaaaatc aaacaggttc
     3001 gcgcctggtt tactggcggt gcgacagcag aaaaaggcgc tggcggcggt gattatcacg
     3061 accagggggc gaatcactgg attgatgatc atattgccac cccgatgagt aaataccgcg
     3121 attacgagca gtcacgccag tcatttggca ttaacgttct tggcacgttg attgttgaag
     3181 ttgaagcaga aaacggccag accggattcg ctgtttcgac agccggtgaa atgggctgtt
     3241 ttattgtcga aaaacatctt aaccgtttca ttgagggtaa atgcgtcagt gatatcaaac
     3301 tgatccacga tcaaatgctc aatgccaccc tgtattactc cggctctggt ggcctggtga
     3361 tgaatacgat ttcctgtgtc gatctggctc tgtgggatct gttcggcaaa gtggtcgggc
     3421 ttccggttta taaactttta ggtggtgcag ttcgtgatga aattcagttc tacgccacag
     3481 gtgcgcgtcc ggatctggca aaagagatgg gctttatcgg tggcaaaatg ccgacgcact
     3541 gggggccaca tgatggtgat gcggggatcc gcaaagatgc cgctatggtc gcggatatgc
     3601 gtgaaaaatg cggtgaggat ttctggttaa tgctcgactg ctggatgagt caggacgtga
     3661 actatgccat caaactggcc cacgcttgcg cgccctataa cctgaaatgg atcgaagagt
     3721 gcctgccgcc acagcagtat gaaggttatc gcgaactgaa acacaacgca ccagcgggga
     3781 tgatggtcac cagcggtgag caccacggca cactgcaatc tttccgtacg ctttcagaaa
     3841 acggtatcga cattatgcag ccggatgttg gctggtgcgg tggcttaacc acgctggtgg
     3901 aaattgccgc aatcgccaaa tccagggggc aactggtggt gccacacggt tcgtctgttt
     3961 actttactcc caccatgcgg tgatcacctt caccaatacg ccattcagcg aattcctgat
     4021 gaccagcccg gattgttcaa cgatgcgtcc gcaatttgat ccgattctgc ttaatgagcc
     4081 agtaccggtg aatggtcgta ttcataaatc agtgcttgat aaacccggtt tcggcgtcga
     4141 actcaatcgt gactgcaatt tgaaacgccc ctacagccac taatcactcc gggcgttgct
     4201 gtgacaacgg taacgcccac atattctaag gagcttttta tgagcaccgc tttgcttgac
     4261 gccgtggtga agaaaaaccg cgcgcgttta attccgttta tgttggcgct gtatgtgctg
     4321 gcgtttctcg accgttcgaa tattggtttt gccaaacaga cctaccagat tgataccggg
     4381 ctgagtaatg aagcttatgc gctgggagca ggcattttct ttgtggtata cgcgtttctg
     4441 ggggttccgg cgaatctttt gatgcgcaaa ctgggggcca gaacctggat tggtacgaca
     4501 acactgctgt ggggatttct ttcggctgcc atggcatggg ccgatactga agcgaaattt
     4561 ctgattgttc gcactctgct tggtgctgcg gaggccgggt ttttccctgg tatgatttat
     4621 ctcacctcgc aatggtttcc gcagcgtaat cgcgccagca ttatggggct gttctatatg
     4681 ggcgcaccgc tggcgttaac actgggatca ccgctttccg gcgcgctgct ggagatgcat
     4741 ggatttatgg ggcatcccgg ctggttctgg atgtttgtta ttgaaggatt gttggcagtc
     4801 ggcgctgggg tattcacatt cttttggctt gatgacacac cggagcaggc acgttttctg
     4861 agtaaacaag aaaaaacgtt gcttatcaat caactggcaa gtgaagaaca acagaaagtg
     4921 acttctcgat tgagcgatgc gctgcgtaat ggccgagtct ggcaactggc gattatctac
     4981 ctgaccattc aggtagcggt ttacggatta attttcttcc tgccgaccca ggttgcggca
     5041 ttgctgggaa caaaagtggg ctttacggcg tcggtggtca ccgccattcc gtgggttgcg
     5101 gccttgtttg ggacctggct tattccgcgc tactccgata aaaccggcga acggcgtaat
     5161 gtcgcagcgc tgacattact ggcggcgggc attggtattg gtctgtccgg gctgctttct
     5221 ccagtactgg cgatcgtagc actgtgtgtt gcagccatcg ggtttattgc cgtgcaaccg
     5281 gtgttctgga cgatgccgac acagcttctt tccggtacgg cgctggctgc ggggattggt
     5341 tttataaacc tgtttggtgc agtgggcggg tttattgccc cgatcctgcg cgtgaaagca
     5401 gaaacgttat tttccagcga tgcggcggga ttactgacgc tggcagcggt ggcggtcatc
     5461 ggttcgctga ttattttcac tctgcgtgta aatcgcactg ttgcgcagac cgacgtggca
     5521 catcattaaa tagtttaagg aacacgatga acgcattatt aaccaatccc tttaaagaac
     5581 gtttacgcaa gggcgaagtg caaattggtc tgtggttaag ctcaacgact gcctatatgg
     5641 cagaaattgc cgccacttcc ggttatgact ggttgctgat tgacggggag catgcgccaa
     5701 acaccattca ggatctttat caccagctac aggcggtagc gccctatgcc agccaccccg
     5761 tgatccgtcc ggtggaaggc agtaagccgc tgattaaaca agtcctggat attggcgcgc
     5821 aaactctact gatcccgatg gtcgatactg ccgatcaggc acgtcaggtg gtgtctgcca
     5881 cgcgctatcc tccctacggt gagcgtggtg ttggggccag tgtggcacgg gctgcacgct
     5941 ggggacgcat tgagaattac atggcgcaag tcaacgattc gctttgtctg ttggtgcagg
     6001 tggaaagtaa aacggcactg gataacctgg acgaaatcct cgatgttgaa gggattgatg
     6061 gcgtgtttat tggacctgcg gatctttctg cgtcgttggg ctacccggat aacgccgggc
     6121 acccggaagt gcagcgaatt attgaaacca gcattcggcg gatccgtgct gcgggtaaag
     6181 ctgctggttt tctggctgtg gctcctgata tggcgcagca atgcctggcg tggggagcga
     6241 actttgtcgc tgttggcgtt gacacgatgc tctacagcga tgccctggat caacgactgg
     6301 cgatgtttaa atcaggcaaa aatgggccac gcgtaaaagg tagttattga tatcaaaggc
     6361 ccatggggat cggctgtggg cctgtgttaa ttagtggtta ttcgctgcca gatcggcttc
     6421 gcttagctga gtggccgcga gcacctggtc acgatcaa
//
LOCUS       DADSZD010000211         6386 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.213, whole genome shotgun sequence.
ACCESSION   DADSZD010000211 DADSZD010000000
VERSION     DADSZD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.213"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(182..1852)
                     /gene="betA"
                     /locus_tag="J1Z28_004037"
     CDS             complement(182..1852)
                     /gene="betA"
                     /locus_tag="J1Z28_004037"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HBA7718812.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTTVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVITSKPGVNPLFEA
                     MIEAGMQAGYPRTDDLNGYQQEGFGPMDRTVTPHGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRAAANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPRIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPGILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGKEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1866..3338)
                     /gene="betB"
                     /locus_tag="J1Z28_004038"
     CDS             complement(1866..3338)
                     /gene="betB"
                     /locus_tag="J1Z28_004038"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HBA7718813.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTD
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPTKCKAAFEQKVLARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGIEEGARVLCGGNVLKGDSFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSILTYESEDEVIRRANDTDYGLAAGIVTADLNLAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3352..3939)
                     /gene="betI"
                     /locus_tag="J1Z28_004039"
     CDS             complement(3352..3939)
                     /gene="betI"
                     /locus_tag="J1Z28_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HBA7718814.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAERRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRHQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4068..6101
                     /gene="betT"
                     /locus_tag="J1Z28_004040"
     CDS             4068..6101
                     /gene="betT"
                     /locus_tag="J1Z28_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HBA7718815.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKNLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1328 a         1731 c         1761 g         1566 t
ORIGIN      
        1 taatattgta ttaatagtct gtatcaggaa tgttcgggtt aaatatcaac gaaaaaccag
       61 catcatgaat actggatatg aagcatgaga gttacctcag tgttttatat aaggattcgg
      121 tcctcatagt agaacggtaa ctctcaatct gatcggttcc tgagtcagtt cacatcacga
      181 ctcatttttt cgctctcacc ggcatcccat ttgccacaaa atatcccgcc gtgctcctcg
      241 gcaacgcttc cttcccacga atcatatccg ctattttctc gccaatcata atggtcgtgg
      301 cgttcaaatt gccggtgata atttgcggca taattgacgc atccaccacg cgcaggcctt
      361 ccagcccgtg tacgcggcct tcgccgtcaa ccacagccat ctcgtcataa cccattttgc
      421 aggtaccgca cggatgaaac gcggtttcgg cgtggttacg cacgaactca tcgagctgct
      481 catccgtctg gcattcgaca ccagggctga tttcgcggcc acggtattga tccagcgcgg
      541 gttgatgcat gatctcgcgg gtgatgcgaa ttgcgtcgcg gaactcctgc cagtcctgct
      601 cgtgcgacat gtagttaaac agaattcccg gatgctggtg cgggtcgcgg gatttaatcc
      661 gcacatgccc acggcttggc gagcgcatcg atccgacatg gcattggaag ccgtgctctt
      721 tcactgcgtt cgagccgtta tagttaatcg ccactggcag gaaatggtac tgaatattcg
      781 gccacgcaaa ttcctcacgg ctgcgaataa atccgcctgc ttcaaagtgg ttgctggcac
      841 caacgccggt gccgccaaac agccactccg caccgattct cggctgattc caccactgca
      901 gagcagggta gagggaaacc ggttctttgc actcgtattg cagatacatc tccaggtgat
      961 cctgaagatt ttcgccgacg ccgggtaatt catgcaccag cggaatatca aactccgcca
     1021 gcagttcagc gttgccgacg ccggagcgtt gcaggatctg cggtgaagca atcgcgcctg
     1081 cacataacag cacttcttta ttcgccgccg cgcgggtcgg aatggtgctg tcgccttcca
     1141 gccactcgac gcccaccgcg cgtttgccgt caaaaatgat gtgatcggtc atggcgtgag
     1201 tacgaatggt caggttaggg cgcgatttgg cctgatcgag atagccacgc gcggtgctgg
     1261 cgcgacggcc atgcggcgtg acggtgcgat ccatcgggcc aaaaccttcc tgctgatagc
     1321 cgttgagatc gtccgtgcgc gggtagcctg cctgcatgcc cgcttcaatc atcgcttcaa
     1381 acagcggatt gacgccgggt ttggaggtga tgacgctcac cgggccatcg ccaccgtggt
     1441 aatcgttctc gcccacatcg cgagtctcgg ccttgcggta gtagggcagg cagtcgagat
     1501 aactccagtt ctccagacca ggttcttgtg cccagttatc gagatccagc gcattgccac
     1561 ggatgtagca cataccgttg atcagcgatg aaccgcccag acctttgccg cgtccgcact
     1621 ccatgcggcg gttgttcata aacggttcgg gttccgtctc ataggcccag ttgtagcgtt
     1681 tgccctgtag cgggaacgcc aacgcggcgg gcatctgggt gcggaagtca aagcgatagt
     1741 ccgggccgcc tgcttcaagc agcagcacgg ttgtgttcgg atcttcagtc agacgggtag
     1801 cgagaacgtt gccggctgag ccggcaccaa taatgatgta gtcaaattgc aaataaacct
     1861 cctggttaga atatggactg gaatttagcc atctcaacct ggatggactt cacctgggtg
     1921 taactctgga gcgtcatcac gccgttctcg cgaccaatgc cggagtgttt gtagccgcca
     1981 acgggcatct ctgccgggga ttcgccccag gtgttgatcc agcaaatacc cgcttccagc
     2041 tgatgaatga cgcgatgcgc gaggttcagg tccgccgtca cgatacccgc cgccagaccg
     2101 tagtcggtat cgttagcgcg gcgaatgact tcgtcttccg actcgtaggt cagaatggac
     2161 atcaccggcc cgaaaatctc ttcgcgcaca atggtcatat cgtcgctgca atcggtgaac
     2221 acggtcggtg caacccatgc gccgttatcg aagctatccc ccttcagtac attgccgccg
     2281 cacagtacgc gcgcgccttc ctctatgcct ttggcgatat agcgcagcac gttatcgcga
     2341 tgcgggaagc tgaccagcgg gccgaagtta gtttgcggat cgaaaacgtc gcccgcgcga
     2401 atgcgctcaa cgcgcgccag aaccttttgt tcaaatgcgg ctttgcattt cgtcggaacg
     2461 aagacgcgag tgccgttggt acacacctga ccggagctga agaaattcgc catcatggcg
     2521 atatcggcgg cgagatcgag atccgcatcg tcgaaaacga tcagcggtga tttaccgccc
     2581 agctccatgg tcacttcttt cagggaagag gctgccgagt tagccatcac ttttttgccg
     2641 ctggcgacgc cgccggtaaa tgacactttg gcaatgcccg gatgatcggt cagatattgt
     2701 ccggtctccg cgcccacgcc cggcaacacg ttaaatacgc cgtccggcag gcccgcttcg
     2761 ctgtaaattt cggccagctt taacgcggta agcggggtga cttcgctcgg tttgaaaatc
     2821 attgcattgc ctgccgccag cgctggggcg gatttccaca gggcaatctg aatcgggtag
     2881 ttccatgcgc caatccctgc cactacgccc agcggttcgc ggcgggtata aacaaatgac
     2941 gtctcacgca acgggatctg gctgccttcc agcgccggga tcagcccggc gtagtactcc
     3001 agcacgtccg caccggtaac gatatcgacg gttgaggttt ccgaatatgc ttttccggtg
     3061 tcgagggttt ccagttttgc gagttcgtca ttgcgttcac gcagaatatc aacggcccga
     3121 cgcagaatac gcgagcgctc catagcggtc atcgccgccc agattttttg cccttgttgg
     3181 gcactcttca cggcgcgatc gacatcctca cgcccggcgg cctgcacggt cgccagcacg
     3241 ttaccgttgg ccgggttaat ggtctcgaag gtgcgaccgc tggtggcgga ggtataacca
     3301 ccatgtatat aaagctgctg ttctgccatt cgggacatca cgtctcctcg gttaatcggt
     3361 gggtaaatgc tgagtaataa agtggcgggt cagcgagtgt gcgagaggtt tatccagcgg
     3421 tttgccgctc agtgccgcgc gcagccataa tccgtcaatc agcgcggcaa ggccgtagcc
     3481 cgcttcctgt gcctggtggc gcggcaattc gcggcgaaac tcgctcacca gattcgacag
     3541 caagcggcgg ctgctgacct gctgtaaacg atagagcatc ggctgatgca tactgctggc
     3601 ccagaaggcc agccaggctt tcatcgccgc actgctcacc tgggtttcat cgaagtttcc
     3661 gccaacaatc gcctgtaatc gccgctctgc actgccctgc ggaagtgcat gtaatcgatt
     3721 caaaaccgcg tcacgcagct gactggtgat atcgcgcatg gttgcttcca gcagaccatt
     3781 tttgtccctg aaatagtggc tgatgatccc cgtagaaacg cctgcacggc gggcgatctg
     3841 cgcgatcgtt gcatcgtgca tgcccacttc atttattgct tccagcgtgg cgtcgatcag
     3901 ttgtctgcgc cggatcgact gcattcccaa tttgggcatt ttcgccactc cattcatcag
     3961 cgttgtttat ctattaaagc ggttattgat tggacgttca atataaaatg tgtcttaatt
     4021 gttactaatt tgattttaaa tggtaacaat aacagtgggg atactggatg acagaccttt
     4081 cacacagcag ggaaaaggac aaaatcaatc cggtggtgtt ttacacttcc gccggactga
     4141 ttttgttgtt ttccctgaca acgatcctgt ttcgcgactt ctcggccctg tggattggcc
     4201 gcacgctgga ctgggtttct aaaaccttcg gttggtacta tctgctggcg gcaacgctct
     4261 atattgtctt tgtggtctgt atcgcttgtt cgcgttttgg ttcggtgaag ctcgggccag
     4321 aacaatcaaa accggaattc agtctgctga gttgggcggc gatgctgttt gctgccggga
     4381 tcggtatcga cctgatgttc ttctccgtag ccgaaccggt aacgcagtat atgcagccgc
     4441 cggaaggcgc gggacagacg attgaggccg cgcgtcaggc gatggtctgg acgctgtttc
     4501 actacggctt aaccggctgg tcgatgtatg cgctgatggg catggcgctt ggatacttta
     4561 gctatcgtta taatttgccg ctcactatcc gctcggcgct gtacccgatc ttcggtaaac
     4621 ggattaacgg gccgataggt cactcagtgg atattgctgc ggtgatcggc accatcttcg
     4681 gtattgccac tacgctcggt atcggtgtgg tacagcttaa ctatggcttg agcgtgctgt
     4741 ttgatattcc cgattcgatg gcggcgaaag cggcactgat cgccttgtcg gtgatcatcg
     4801 ccactatctc ggtgacatcc ggtgttgata agggcattcg cgtgttatcg gaactcaacg
     4861 ttgcgctggc gctgggattg attctgttcg tgttgtttat gggcgacacc tcgttcctgc
     4921 ttaatgcact ggtgctgaat gttggcgact atgtgaatcg ctttatgggc atgacgctca
     4981 acagttttgc cttcgaccgt ccggttgagt ggatgaataa ctggacgctc ttcttctggg
     5041 catggtgggt ggcatggtcg ccgtttgtcg gcttgttcct ggcgcgtatc tcgcgtgggc
     5101 gtaccattcg ccagttcgtg ctgggcacgt tgattattcc gtttactttc acgctgttat
     5161 ggctctcggt attcggcaat agcgcgctgt atgaaatcat ccacggcggc gcggcatttg
     5221 ccgaggaagc gatggtccat ccggagcgcg gcttctacag cctgctggcg cagtatccgg
     5281 cgtttacctt tagcgcctcc gtcgccacca ttactggcct gctgttttat gtgacctcgg
     5341 cggactccgg tgcgttggtg ctggggaatt tcacctcgca gcttaaagat atcaacagcg
     5401 acgcacccgg ctggctgcgc gtcttctggt cggtggcgat tggcctgctg acgctaggca
     5461 tgctgatgac caacgggata tccgcgctgc aaaacaccac ggtgattatg ggcctgccgt
     5521 tcagctttgt gatcttcttc gtgatggcgg ggttgtataa atctctgaag gtagaagatt
     5581 accgccgtga aagtgccaac cgcgataccg caccgcgacc gctggggctt caggatcgcc
     5641 tgagctggaa aaaacgtctc tcgcgcctga tgaattatcc gggcacgcgt tacactaaac
     5701 agatgatgga gacggtctgt tatccggcaa tggaagaagt ggcgcaggaa ctgcggttgc
     5761 gcggcgcgta cgtggagcta aaaaacctgc cgccggaaga gggacaacag ttggggcatc
     5821 tggatttgtt ggtgcatatg ggcgaagagc agaactttgt ttatcagatt tggccgcagc
     5881 aatattcagt gccgggcttt acctaccgcg cacgtagcgg gaaatcgacc tactaccggc
     5941 tggaaacctt cctgttagaa ggcagccagg gcaacgacct gatggactac agcaaagagc
     6001 aggtgatcac cgatattctc gaccagtacg agcggcacct taactttatc catctccatc
     6061 gtgaagcgcc gggccatagc gtgatgttcc cggacgcgtg attgttattg cataaaaccg
     6121 cgccatgtct gcatatggcg cggtttctct tacctcttga tacgttatat ctgtacagtt
     6181 aagcccttag tgtcgattga tgattgccag gcaacagata ataaaaaaga aaagaactat
     6241 tgaagcccaa aaactacgtt tggactgttg tgaatgttca ataagcttag tcttatttaa
     6301 tgtaaatatt ggtgctaatt tgaaatgacg atttattcat ggggaacgcg tagcgcaaat
     6361 caactaaaat caatgtaacc gtttgt
//
LOCUS       DADSZD010000212         6184 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.214, whole genome shotgun sequence.
ACCESSION   DADSZD010000212 DADSZD010000000
VERSION     DADSZD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.214"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            167..1075
                     /locus_tag="J1Z28_004041"
     CDS             167..1075
                     /locus_tag="J1Z28_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBA7718816.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1294..3285)
                     /gene="tkt"
                     /locus_tag="J1Z28_004042"
     CDS             complement(1294..3285)
                     /gene="tkt"
                     /locus_tag="J1Z28_004042"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA7718817.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3563..4321
                     /locus_tag="J1Z28_004043"
     CDS             3563..4321
                     /locus_tag="J1Z28_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBA7718818.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYAKRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4369..5289)
                     /gene="speB"
                     /locus_tag="J1Z28_004044"
     CDS             complement(4369..5289)
                     /gene="speB"
                     /locus_tag="J1Z28_004044"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBA7718819.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNERSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5425..6156)
                     /locus_tag="J1Z28_004045"
     CDS             complement(5425..6156)
                     /locus_tag="J1Z28_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718820.1"
                     /translation="MKKWKVRNALIALAVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFKSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1511 a         1632 c         1597 g         1444 t
ORIGIN      
        1 atttgtgata cacatctcta ttttcttatt gaatgttcga aaacatactt attacgttta
       61 ttattttttc gctcatcccc tttataaatt ctgaattatc ggctgttgtc tgttttctca
      121 tcaccccatg tgacatattc ctatacttta ctcaaggaag attcatatga aagcacctgt
      181 ttcattaacc tcatttcgcc cacaaaaatc tcttgctatc gccattggcg tgcttgccgt
      241 ggttgtgctg ccatttctct cgtattacac cgttaatgaa ggtgaacgcg gcattttgct
      301 gcgctacgga aaaatcgtaa aagttgccga gccggggctg ggttttaaga tcccttttat
      361 ggaaagcgta gagaaaattt ccacgcgtaa tcaggctgtg gtctatcagg gattacaggc
      421 ctacagccgc gaccagcaac cggcacaaat gacagtatca gtcagcttcc acattaaacc
      481 ttcagaagca ggcgcggttt ataccaccta caacaccatt gaagcattaa aagatcgtct
      541 gattgtccgt cagttgccaa ctcaactgga gaacgttttc ggacaatata ccgccatcag
      601 cgccgtacag gatcgtacaa aactggttca ggatctgcaa aatgccatgc gcaaagccgt
      661 tgttgggcca gtggtaattg acggggtcca gattgaaaat attgattttt ctgacgctta
      721 tgaaaagtcg attgaagacc gaatgaaagc agaagtcgcc attgccacgc gtaagcaaaa
      781 tctggaaacg gagaaaatcc aggcgcaaat agcggtaact caggcccagg cggaagcaga
      841 cagtaaacta gcggcagcca aagcagaagc ggaaactatt cgcgtaagag gcgcggcaga
      901 ggctgaaact attcgtttaa aaagtgcggc tgaagcagaa gctatccgcc tgcgcggtga
      961 agctctgcgg gagaatcccg gacttgttgc cctcaccact gctgagcgtt gggacggtaa
     1021 actgcccgat accatgatcc ccggcagcac agttccgttt atctctacta agtaaaaaag
     1081 ggccgctttc gcggcccttg agactgatag caaagacaaa actgcctgat gcgctacgct
     1141 tatcaggcct acatacttcc tgcaatatgt tgaatttgca aaatcttgta ggacggataa
     1201 ggcgttcacg ccgcatccgg cataaacaac gcgcactttg ccaacaatct gaagggccgc
     1261 atttgcggcc cttctcacaa agcatcttac cgattacagc agcgcttttg ctttcgcaac
     1321 aacgttgtca acggtgaagc cgaactcttc aaacagctgc tctgccggag cagattcacc
     1381 gaaggtggtc atgccgacga tagcaccgtt caggccaaca tacttgtacc agtagtcagc
     1441 aatgccagct tccaccgcaa cacgagcggt aaccgctttc ggcagtacgg attcacggta
     1501 agccgcgtcc tgcttgtcga atgcgtcggt agacggcatg gaaaccacgc gcgctttcac
     1561 gccttcggca gtcagttttt cgtaggcggc aacagccagt tcaacttcag aaccggtcgc
     1621 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1681 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1741 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gctactgcgg attcaacctg
     1801 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga tctgttctac
     1861 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1921 ctgacgctgt ttcatcagcg cagccatacg tacggcatta cgtgcgtatt ccacgaacat
     1981 caggaaggtt gaggtatacg gcaggaaacc accatgcagg gagataccgt tggcaatcgc
     2041 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     2101 tgctttagaa ccagaccaca gggtcaggtt agacggagca aggtcagcgg agccgccgag
     2161 gaattccggc aacagcggac cgaacgcttc gatagcattc tgagacgctt tacggctggc
     2221 gattttcgcc ggattagcct gaagtttagc aatgaactct ttcgctttgg cgtcgaagtc
     2281 agacggcatt tcgcctttca tacggcgggt aaattcagcg gcttcctgcg gataagcttt
     2341 cgcgtaagca gcgaatttct cgttccatgc ggactctttc gcctggcctg cttctttcgc
     2401 atcccactga gcatagattt cagacgggat ttcgaacggc gcgtatttcc agcccagctg
     2461 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgggtacc
     2521 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2581 agtcactgcg cgcgcttctt ctactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2641 atcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gcggtgtcgt cggtaaacca
     2701 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2761 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2821 gtcgcccatg aaggcgtagg tgtagtggtc gacgatgtcg tggcccggac ggttaaactg
     2881 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2941 cggaccggtg gtggtttcta cacctgcggt gtaacccact tccgggtgac ccggagtttt
     3001 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     3061 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     3121 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     3181 gtcagccata cccatcgggg cgcccgggtg accggatttg gctttctgta ctgcgtccat
     3241 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3301 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3361 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3421 cagaaatctc tggatcatgc tcgcatgttg cgcaatccac tcgcccgtcc gctgcgcttt
     3481 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3541 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3601 tacggtgctg accggttgcc agaatatgga ctccagtgga ctgctctcat caggagcgga
     3661 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa aaactgagcg atcaggcatg
     3721 ccaggagatg gacagcaaag cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3781 gacgacaatt tctcgcgcgt taggcgataa catcaacggt cagccggtga attacaaagt
     3841 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3901 cgggctgatg gatatgatga cggataacga agtcgaagcg gtgctcggtc acgaaatggg
     3961 gcacgtggca ttaggccatg tgaagaaagg aatgcaggta gcacttggta caaatgccgt
     4021 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaactcgg
     4081 tgatctgggc gagaaattag ttaattcgca attctcccag cgccaggaag cagaagccga
     4141 tgattattct tacgatcttc tgcgccaacg cggcatcagc ccggcaggtc ttgccaccag
     4201 ttttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4261 agcatccgcc gaacgcgccc agcatattcg cgatcgtatg agcgcggatg gagttaagta
     4321 agcattttat tacgccggat ttgacgcata acgcatctta tctggcgctt actcgccctt
     4381 tttcgccgcc tggatataca gcatctccag cgccagcgtc gccgcagcca gagcggtgat
     4441 ttccgactga tcgtatgccg gagccacctc cactacgtcc atcccaacga tgttgagatc
     4501 tttcaggccg cgtactagtt taatggcgcg atcggaggtc aggccgccaa ttaccggcgt
     4561 accggtgcct ggtgcaaaag caggatccag gcagtcgata tcaaaggtca ggtaaaccgg
     4621 catatcaccc acaatctgtt tcacctgggc gataatgtca tccacgctgc gctcgttcac
     4681 ctggcaggcg tccagcacgg taaagccgtt gtctttatca aactcggtac gaataccaat
     4741 ctgcacggaa tgattcgggt cgatcagacc ttctttcggc gcggtataga acatggtgcc
     4801 gtggtcaaat tcacaaccgt tagcgtaggt atcggtgtgg gcatcaaagt gtaccaacgc
     4861 cattttgccg aaatgcttcg catgagcgcg cagcagcggc agcgtaacaa agtggtcacc
     4921 accgaaggag agcatacgct taccggcagc cagcagcttc tcggcgtgcg cctgcagctt
     4981 ctcgctcatc tcacgggcat cgccgaaggc atataccaga tcaccgcagt ccacgacgtt
     5041 caggcgctca cgcatgtcga aattccacgg gaagcggttg tgttcccagg ccagattcgt
     5101 cgaaacctga cggatagctg ccggaccgtg gcgccccccc gcacggccag aagtggccat
     5161 atcgaacggc acgccagtaa tcacccagtc agcatcgctg tcatacggct ggaagttcat
     5221 cggcaggcgt aaaaaaccaa aggcgttgga aaccagtgag ttatcgtatt gatgacctaa
     5281 ggtgctcatg gatggactcc tattacaaag tcgatataaa aaaacccctc cgcgtcgtta
     5341 ggcccgacga ggaagggttg gatttgcgaa acacaagttt gtgctaatta tcgccctaaa
     5401 tgggcaaggc atcaagcaag gttactattt ccgacacggt ggcggcgcat agccattgtt
     5461 atctgccgga aacgcttgtc cagagcacgg cggcagcgcg ttttgcgccg ggccttgtaa
     5521 ttcaaaggcg gcaaagccga ttacaccggt attacgcaca tcgccttgcg ccgaattggc
     5581 tgcgtaggaa tcagcaacgt tggcgaactg aaaagccgct tcggtatgtt tatctttgcg
     5641 gaatccttta atcgctaacg aatctccggc attcacaata taaccgttgt tattcagcga
     5701 accttgttta ccattcagaa catccaggcc atcaaccgta gcaacgatct cgtagttggt
     5761 gttacgactg taattacgga catataattg ataattcatt ccgactctgg catggacaat
     5821 atagcgtggt tcattattgg atgaatcata aacccgcgta attggtagag atttaaaatt
     5881 accatcgcga atagcatatt ccagttcgtt aatacgaatg ctataaacgc ggtcgtagcc
     5941 ggaaggaaca ttagtcgaat aactaataac gatcatctct gccggatcgc gccaggctcg
     6001 ttcagcagat acgccctgaa ccgttgagtg cacatcccca ccccaggcgg tgccgacatt
     6061 acccgatgca ctgctattat actgcgcatt actggaacaa ccggccagca gcacggctaa
     6121 tgctatcaac gcgttacgta ctttccattt tttcataacc gttccctctg catttaatta
     6181 atgc
//
LOCUS       DADSZD010000213         6175 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.215, whole genome shotgun sequence.
ACCESSION   DADSZD010000213 DADSZD010000000
VERSION     DADSZD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.215"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..205
                     /locus_tag="J1Z28_004046"
     CDS             <1..205
                     /locus_tag="J1Z28_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HBA7718821.1"
                     /translation="KKICRAEGATEEDDNKLVREFERLTEHPDGSDLIYYPRDDREDS
                     PEGIVKEIKEWRAANGKPGFKQG"
     gene            267..410
                     /locus_tag="J1Z28_004047"
     CDS             267..410
                     /locus_tag="J1Z28_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E6 lysis protein"
                     /protein_id="HBA7718822.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSTAELTGV
                     ETQ"
     gene            907..1821
                     /locus_tag="J1Z28_004048"
     CDS             907..1821
                     /locus_tag="J1Z28_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="HBA7718823.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSV
                     KALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     ASARRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPEFSQWLDAVIQRVEMYNASLSV
                     PLSPPECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
     gene            complement(2025..2423)
                     /locus_tag="J1Z28_004049"
     CDS             complement(2025..2423)
                     /locus_tag="J1Z28_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718824.1"
                     /translation="MRISNIEWLKKRLGFIRKLGEQTARQRQIIDLLDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKKIKYDLETSPNPDA"
     gene            2599..3525
                     /locus_tag="J1Z28_004050"
     CDS             2599..3525
                     /locus_tag="J1Z28_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBA7718825.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPDRGGAKKDSG
                     NLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARTVGHDIIEWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDGVSRQERTEPERVKSPSRGFSMGF"
     gene            3528..4061
                     /locus_tag="J1Z28_004051"
     CDS             3528..4061
                     /locus_tag="J1Z28_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718826.1"
                     /translation="MASTEEKLLALLLSVEHLQHTAMEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            4282..6117
                     /locus_tag="J1Z28_004052"
     CDS             4282..6117
                     /locus_tag="J1Z28_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HBA7718827.1"
                     /translation="MSGGDGRDHNSGAHNTGGNINGGPTGLGGNGGASDGSGWSSENN
                     PWGGGSGSGVHWGGGSGNGNGGGNGNSGGGSNSSVAAPMAFGFPALATPGAGTLGIAV
                     SGEALSAAIADIFAALKGPFKFSAWGIALYGILPSEIAKDDPNMMSKIVTSLPAETVT
                     DAQVSSLPLDQATVSVTKRVTDVVKDERQHIAVVSGVPMSVPVVDAKPTSTPGVFSTS
                     FPGVPSLTVSTVNDAPVSTSLPAGVTEDKDRTAFPAGFTFGGGSHEAIIRFPADSGQK
                     PVYVSVTDVLTPEQVKQRQEEEKLRQQEWDATHPVEVAEREYESAKAELEAEDKKVQS
                     LQATLDGLKNTPEGLALSDAGQHPLTSSESRFVAIPGYSGGGVHFDATATVDSRDRLN
                     SLINLGATAYVNNVLELGDVSAPTEDGVKVGNAIKNATIEVYDKLRQRLLDRQNEINN
                     AQASLNTAIESRKPKEKKVQDAKDKLDKENRRKQPGKATGKGKTVGDKWLDDAGKDSG
                     SPIPDRVADKLRDKEFKSFDDFRKKFWEEVSKDPELSKQFKDSNKTNIQKGKAPFARK
                     KDQVGGRERFELHHDKPISQDGGVYDMDNIRVTTPKRHIDIHRGK"
BASE COUNT         1613 a         1359 c         1695 g         1508 t
ORIGIN      
        1 aaaaaaaata tgtagagctg aaggtgctac tgaagaggat gacaataaat tagtgagaga
       61 gtttgagcga ttaactgagc acccagatgg ttcagatctg atttattatc ctcgcgatga
      121 cagggaagat agccctgaag ggattgtcaa ggaaattaaa gaatggcgag ctgctaacgg
      181 taagccagga tttaaacagg gctgaaatat gaatgccggt tgtttaagga tgaatgactg
      241 gcattctttc acaacaagga gtcgttatga aaaaaataac agggattatt ttattgcttc
      301 ttgcagtcat tattcttgct gcatgtcagg caaactatat ccgtgatgtt cagggcggga
      361 ctgtatcacc gtcgtcaact gctgaactga ccggagtgga aacgcagtaa cccgaaatcc
      421 tcatttgaca agagcaaagc gtgtcaggct gatcctgacg cgcttttttt tgaagtttca
      481 caaaattcca tgcgggagat gagagctaaa gtctccgtgc agaacttttc atccaggggg
      541 agaaaacttg tcattttgac ctgttcgccc ttcggaagag tcgaaatcga tcgcgcatcg
      601 ctttcgtgca tagttatgca ggcctctaaa aacgattctg acgcgttttt ctggtttagc
      661 ttggtgtttt cttgtctttt tgcgtttttt gcgccagaaa gcgattgagg gcgttttaag
      721 gggttcgtac aacgggagtt atggtaaatg gatcgggttt tcgggaaggt tcgacaggat
      781 ttgccgttgg gtgtagtgta agcgactgaa aaacaaacgc ccctgaaatc atgtccagat
      841 ccggcaagat tcaacatgaa atacagaggg cgtgtaggtc gcataacccg gagagaatcg
      901 taacacatga gcgccgcgct tcaatacttc gacgaaaatt taccccaccg tccctatcac
      961 acggatgatc tcgcttttgg tcttcgcatc tccggcaaag ggcgtgcgct tcttgcgcgg
     1021 tacatacagc agaaccagcc tcatgcgcag ttctggctgg tttttgacgt tgaccgcgag
     1081 ggggcagcga ttgactggag tgaccggaac gctcccgcgc cgaacatcac cgtaaaaaat
     1141 cctgtgaacg gtcatgctca cctgctctac gcgctcaata tcgccgtgag aaccgcgccg
     1201 gacagctcgg ttaaggcgct gaaatacgcc gctgcgattg aacgttcgct gtgtgagaaa
     1261 ctttgtgctg atgtgaacta cagcggactg atttgtaaaa atccgttcca cctggaatgg
     1321 caggtgatgg agtggcgaga ggaagcctat accctcgatg aactggctga ttatctcgat
     1381 ttgagcgcct cagcgcgccg tagcatcgat aaacattacg ggatggggcg aaactgccac
     1441 ctgttcgaaa tgacgcgcaa atgggcttac agggctattc gtcagggctg gcctgaattc
     1501 tcacagtggc ttgatgctgt gattcagcgt gtcgaaatgt acaacgcatc gctttccgtt
     1561 ccgctttcac cgcctgaatg tcgggctatt ggcaggagca ttgcgaaata cacgcacagg
     1621 aacttcacgc cggaaacttt cgcacagtat gtggctgata cgcacacacc agaaattcag
     1681 gctgcacgcg gtcgcaaggg cggttctaag tctaagcgca gcacagtggc tacatccgca
     1741 cgcacgctga aaccgtggga ggcattaggg attagtcgcg cctggtatta ccaactgaaa
     1801 aaacgaggtc tcgtagagta gaccaaataa gcctatatca gataacagcg cctttttggc
     1861 gtctttttga gcagcaggtt ttttgccgtt tttccccgtt cagccctgca atggtgctgt
     1921 tttccgaatg attgcgctgg tagtgctcgc cgcagtctca tgaccgagcg tagcgagcga
     1981 atgagcgagg aagcaccagg gaaccggaac acggatggag gcacttacgc gtctgggttt
     2041 ggggatgtct ccagatcgta cttaattttc ttaagtgcct gtaatatcct gtctttttta
     2101 tagcatagct cgcctgtttt tgtgtctacc agtcctgctt cgatcagtag cccagccgcg
     2161 agaaagagtc ggtgattgcg ttctcgtcgt tgtttttttc cctcgattcg tttttggacc
     2221 gcctgttttc gttcgctctc ctgtgcctgt aagtcgtttt tttccgctgt tgccagtacg
     2281 tggaggagtt tgcgttcctg ttctgtgagt cctgcttcgt tgtcgagcag gtcaataatc
     2341 tgtctctgtc gtgcggtctg ttctccgagc ttcctgatga atccgagccg tttcttcagc
     2401 cattcgatgt tgctgatgcg catgttcgtg atgctccctg gtgttttttg cggtgtgttg
     2461 tggtcgtgca gtatatcgct tgcggtcggt gggggctggg gttatcttcc gtactaattc
     2521 acataattag aaggaagcgc acttatatgt ttctctcctt cgtcgagaaa cagtgcgctc
     2581 ctgcggaggg ctgaaaccat ggcaacctat cacctcagcg ttaaatttgg tggcaagggc
     2641 caggctgcaa accacgccga ttacatcgag cgcaaagaga aataccgcga ccgtcaggat
     2701 ttggaatatt ccgcacacgg aaacatgccg gaatgggcgc gagataatcc ctcacatttc
     2761 tggcaggcgg ctgaccagtt tgaacgtgcg aacggttcga cctatcggga acttgaaatt
     2821 gcattacccc gtgaactgac accggaacag cgccttgaac tggtgcagga ttttgtacgt
     2881 caggaagctg gcgaacggca cgcttggagt tttgccattc acaatccgaa agcgagcatt
     2941 gacggcggcg aacagcctca tgcgcatatc atgatgagcc agcgtgtgaa tgatggcatt
     3001 gaacgcacac cggaacagta tttccggcgc tataacgcca ggtatcccga ccggggaggt
     3061 gcgaaaaagg attccggaaa cctgaccccc actcagcaaa aagagcagtt gcgggaattg
     3121 cgaaaacgct gggaggtgaa gcacaacgaa catatgagaa aacatggctt tgaaagggga
     3181 ttcattgact gccggacgct gaaagaacag ggcattgaac gacgtcccga agtgcatctg
     3241 ggttttgaag ctgccggacg gcttgatgat gcccagcgtc atgacatcat gcaaaaacgg
     3301 tctgaaccgg actattcccg ccatcttgaa caggcgcgaa cggtcgggca tgacatcatt
     3361 gaatggctgg acactccggt taagcaacat gaactggtca gaaggctgca acatgatgtt
     3421 gcgcgggagc atggctttga catgagagac ggagtgagca gacaggagcg aactgaacct
     3481 gaacgggtta aatcaccgtc acgcggattt tctatgggat tctgaacatg gccagcacag
     3541 aagaaaaatt actcgcgctc ctgttatcgg tggagcatct gcaacatacc gcaatggagc
     3601 aacagaaagc tgttgctgat tctctggctg tggcaaggga aacacttcgc ggatatcggg
     3661 atatttctga cggtgtggca aaacaggtca gggatgaagt cagaaatgag gtgcagaagc
     3721 tggacgtggc agggctgata ggtgagcgca tcagccagga ttttcagcgg ctggaagagt
     3781 cagtcgcaaa catgaaggca aacgcggatg ccctgaacag ggatattgcg acaattcaca
     3841 gcaatatcgt gcaggaatac aacaccctta aagggtatag ctggaaatgg ctgggtgggg
     3901 tgtcagtggc ttttctggtt atgttctgtg tgctcggatg gggaatgaaa acaatgctcg
     3961 acaggaacta caacgccaca gcggcggtgt atcagaaagt tgtgacactg gaagataaac
     4021 tggtggagaa acctgttacg ggtaagagtc ggaaaaactg acgcgcttcg cttgtccaac
     4081 gcctcacgac ccgcgaaagg atttcgcgtt ccgttcaccg ttttcatgag tgattcctcc
     4141 gaactgccaa aaaatatccc cccctgactt gacagaaaaa ataatggcga gtactttttg
     4201 atctgtacat aaaaccagtg gttttatgta cagtattagc tatgtaatca attgttttaa
     4261 cgcttaaaag agggaatttt tatgagcggt ggagatggac gcgaccacaa cagtggcgca
     4321 cataacacag gtggtaacat taatggcggc cctacggggc ttggtggaaa tggcggtgct
     4381 tctgacggtt ctggatggag ttcggaaaat aacccatggg gtggcggttc cggtagtggt
     4441 gttcactggg gaggtggctc cggcaatggc aatggcgggg ggaatggtaa ttccggtggt
     4501 ggcagcaatt catccgtagc agcacctatg gcatttggtt ttcctgcttt ggcaactcct
     4561 ggtgccggaa cactgggtat cgccgtctct ggtgaggctt tatctgcggc aatagcggat
     4621 atctttgcag ccctgaaagg cccgtttaaa ttcagtgcat ggggtattgc gctttacggc
     4681 attctgccat ctgaaatagc aaaagatgac ccgaatatga tgtcaaagat tgtgacgtca
     4741 ttaccggctg aaacggtgac agatgcgcag gtcagttctc tgcctctgga ccaggcaact
     4801 gtcagcgtga cgaaacgtgt gacagatgta gtgaaggacg aacgacagca tattgccgtt
     4861 gtttcaggtg taccaatgag tgtacctgtt gtggacgcaa aaccaacaag cacccccggt
     4921 gtattcagca catcatttcc tggtgttcct tccctgactg tcagcactgt caacgatgct
     4981 cctgtatcaa cttcgcttcc cgccggagtt actgaagata aggatcggac ggcttttcct
     5041 gctggcttta ccttcggtgg tggctctcat gaggcgatta tccgtttccc tgctgacagt
     5101 ggtcagaaac cggtgtatgt gtcggtaacg gacgttctga cacctgagca ggtaaaacag
     5161 cgtcaggaag aggagaaact ccgtcagcag gagtgggacg ccactcatcc tgttgaagta
     5221 gctgaacgcg agtatgaaag tgcaaaagca gaactggagg cagaagacaa gaaagttcag
     5281 agtcttcagg caacgcttga tgggctgaaa aatactccgg aaggtcttgc tctgtctgat
     5341 gcagggcaac acccgttaac atccagtgaa tcccggtttg tggctatccc cggatacagt
     5401 ggcggtggtg tacattttga tgccacagca acggtggatt cgcgtgacag gctgaacagt
     5461 ctgataaatc tgggggcaac cgcttatgtt aataacgttc ttgaacttgg agacgtcagc
     5521 gccccaacag aggatggggt aaaagtcggt aatgcaataa aaaatgcaac tattgaggta
     5581 tatgacaaat tacgtcagcg cctccttgac cgacagaatg aaataaataa tgcccaggct
     5641 tctcttaata cggctattga aagtcgtaaa ccaaaagaga aaaaagttca ggatgccaag
     5701 gataaactgg ataaggagaa tagacggaag cagccaggta aagccacagg gaaaggaaaa
     5761 acagttggtg ataaatggct tgatgatgcg ggtaaagatt caggatctcc cattccagat
     5821 cgtgttgctg ataaactgcg tgataaagag tttaaaagct tcgacgattt ccggaagaaa
     5881 ttctgggaag aagtgtcaaa agatcccgag cttagcaagc aatttaaaga cagtaataag
     5941 acgaacattc aaaagggaaa agcacctttt gcaaggaaga aagaccaagt aggtggtagg
     6001 gaacgctttg aattacatca tgataaacca atcagtcagg atggtggtgt ctatgatatg
     6061 gataatatca gagtgaccac acctaagcga catattgata ttcatcgagg taagtaaaaa
     6121 tggaactgaa acatagtatt agtgattata ccgaggctga atttctggag tttgt
//
LOCUS       DADSZD010000214         6126 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.216, whole genome shotgun sequence.
ACCESSION   DADSZD010000214 DADSZD010000000
VERSION     DADSZD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.216"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            89..2332
                     /gene="pdeF"
                     /locus_tag="J1Z28_004053"
     CDS             89..2332
                     /gene="pdeF"
                     /locus_tag="J1Z28_004053"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBA7718828.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERISALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREVVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2371..3912)
                     /gene="ppx"
                     /locus_tag="J1Z28_004054"
     CDS             complement(2371..3912)
                     /gene="ppx"
                     /locus_tag="J1Z28_004054"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBA7718829.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3917..5983)
                     /gene="ppk1"
                     /locus_tag="J1Z28_004055"
     CDS             complement(3917..5983)
                     /gene="ppk1"
                     /locus_tag="J1Z28_004055"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBA7718830.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
BASE COUNT         1497 a         1483 c         1506 g         1640 t
ORIGIN      
        1 tagtgatgaa taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc
       61 aggatgtggg ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg
      121 tgataaatgg tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca
      181 cattaatact ttcgcgcata tttcttccgg tgaagttttt ctcttttatc tgccactggc
      241 attgatgatc agcatgatga tgtttttcag ctgggcggca ttgccaggga tcgccttagg
      301 gatttttgtc cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa
      361 ttttattatc attatcattc tctgttgggg cggttacagg gtttttactc cccggcgtaa
      421 caacgtttca catggtgata gccgtttaat ttcccagcga ctattctggc agattgtgtt
      481 tcctgcaacg ctgtttctga tacttttcca gtttgctgcg tttgttggat tactggcgag
      541 cagagaaaat ctggtcggcg tcatgccctt taacctcggg accttaatca attatcaggc
      601 cttgctggtg ggtaatctga ttggtgtccc gctgtgctac ttcatcattc gggtggtgcg
      661 aaatccgttt tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaggt
      721 taccaaaaaa gagttcgcaa tctggctact ggcattaggt gctttattgt tgctgttatg
      781 catgccgtta aatgaaaaaa gcacgatttt tagcaccaat tacaccttgt cattattgct
      841 gcccctgatg atgtggggag cgatgcgcta tggttataag ctgatttcat tgctctgggc
      901 ggtcgtgttg atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta
      961 taccacgcag ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta
     1021 tatggctgta ctggcaaccc gtcagcgagc ggtagtcaga cgcattcagc ggcttgcgta
     1081 tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc
     1141 cccctggtct gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta
     1201 tggcatcatg ctacggattc aatacaagca aaaactttct cactggctgt cacccttgct
     1261 ggaaccgggt gaggatgttt atcagctttc gggtaacgat ctcgcgctgc gactgaatac
     1321 agaatcgcac caggagcgca ttagcgcact ggatagccat ctcaagcaat ttcgtttctt
     1381 ttgggatgga atgccgatgc aaccgcagat tggcgtcagt tactgctatg tgcgctcgcc
     1441 agtgaatcat atctacctgc tgctgggaga gctaaatacg gtcgccgagc tttccatcgt
     1501 gaccaacgcc ccggaaaata tgcagcgtcg cggggcaatg tatttgcaac gcgaattgaa
     1561 agataaagtc gcgatgatga atcggctaca gcaggcgctg gaacacaacc attttttcct
     1621 gatggcccag ccgattaccg gtatgcgtgg tgatgtctac catgaaattc ttctgcgcat
     1681 gaaaggtgag aatgatgaac tgatcagccc cgacagcttc ttgccggtcg cgcacgaatt
     1741 tggtttatcg tcgagtatcg acatgtgggt cattgagcat acgctgcaat ttatggctga
     1801 aaacagagcg aagatgcccg ctcaccgttt tgcgattaat ctgtcgccaa cctcggtatg
     1861 tcaggctcgt tttcccgttg aagtcagtca gctgctggct aaatatcaaa ttgaagcgtg
     1921 gcaacttatt tttgaagtca ccgaaagtaa tgctctgacc aatgttaagc aggcgcaaat
     1981 caccttgcag catcttcagg aattaggctg ccagattgcg attgatgatt tcggcaccgg
     2041 ctacgccagc tatgcgcggc ttaaaaatgt gaatgccgat ctgcttaaaa ttgacggcag
     2101 ttttatccgc aatattgtgt caaatagtct ggattatcag atagtggcgt cgatttgcca
     2161 cctggcgcga atgaagaaaa tgcgggtagt ggcagagtac gttgaaaacg aagagatccg
     2221 cgaggtggtg ctctctttgg ggatcgatta tatgcagggt tatcttattg gtaagccgca
     2281 accgttaatt gatacgctga atgaaatcga acccattcgc gaaagtgcct gaataatgcg
     2341 ggccgacatt tctcgtcggc ccgcaaagca ttaagcggcg atttctggtg tactttcttc
     2401 ttcaattttc aaccgccagc cagccacgcc ttcccagtat tcttgctcct tttccagatc
     2461 aagcagtacc agcgcattct gactaaacca gtcatgcgga aaacgcagtg tccagtgact
     2521 gtcatcggta atcagcgtca atgttggcgg tgtggtggtt gcctgacgtt gattattgag
     2581 taatacgcca aggcgcaata gctgaatcag tggcaggaac tgtttcttct taaacaaggt
     2641 aaagcgcggc agatcgtcga gcttaatcgc tttacggtga tagcgcacca gcgtcgccat
     2701 catcaactgc tgttcctgat taaaacccgg caagtcgctg ttttgcagaa tataagcgga
     2761 gtggcgatgc aaaccgctgt ggttgatatt caacccgacc tcatgcagca tggcggccca
     2821 tcgcagtagc gcttccagtt gcggatgcgc cagcttcggt tgctgttccc gccactgttc
     2881 gtacatttgc atagtggtat ccagcacccg tcgggcctgt tcgctgtcga tgtgatactg
     2941 gttggcgagg ctgctggcgg tgcgactacg cacatcctga tgacggaaac gtccttccat
     3001 ttcatacagt acgccttcgc gtaacgcccc gtcagaaagg cgcagttcac ggatggctaa
     3061 agcatcaaac acaccgcata aaatcgccag tccaggaacg aagactgttt tccgttcttc
     3121 ggaaagaccc ggtaaactca gcgatgcaaa attacggtga cgtaaaactt cttttaccag
     3181 tttttccaga cgttccgggg taattatccc gtctttctcg cccatttcca ttaacacttc
     3241 atgggcggct tttatggtac cggaagcgcc cattgcaaca ttccagccct gaattcgaaa
     3301 ttgccaggtt aacgtttcca gtttttgtgc tgccgccatg cgagcgcgct gaaaattctc
     3361 tttattgatg accccgccag ggaaataaag ctgggcaaag ctgacacaac ccatccggcg
     3421 gctttcaacg agaatcggtt cgaaattttc accaatcacc agttccgtag atccgccgcc
     3481 aatatcaata accagtttgc gacctttctc cggttgggta tgttctacgc ccataaaaat
     3541 cagacgggct tcttcattac cggaaataat ttcaatcggg tagggaatga ccttttccgc
     3601 gcgtttcaga aagtcagtgg cgttcagcgc ctgacgcagt gtatgggtgc caactataca
     3661 gacgctggca ggagaaaacc cttgcagccg ttcggcaaac agcgacagac agtttaaacc
     3721 gcgcgtcatt gcctcttcac tcaacatatt atctggcccc aggccgtccg ccagatgcac
     3781 ccgctgtttc aggcggccaa taatctgcat ggcaccatcc accacacggg ctatgaccat
     3841 gtgaaaactg tttgaaccaa gatcgaccgc agcaaactcc tgcggacgag gggatttatc
     3901 gtgtattggc atagggttat tcaggttgtt cgagtgattt gatgtagtca taaatcgcca
     3961 actgcgcccg tactttgcgg cgattgccgc ggggaacgta gcgattactg agttctttat
     4021 cgatataacg tgctttgact gtatcgctga acaatatgtc gatgatgtcc agcacccgct
     4081 gcttcaggcg cggatcgagc agcggcgtcg ccacttcaat acgataatca atattgcgcg
     4141 tcatccagtc ggcggaagaa aggtagacct ttttatcgcc gccattttca aaaatataaa
     4201 cccggtcatg ttcaaggtaa cggtcaacaa tactgatggc acgaatgttg tcgctaatgc
     4261 cttccagatt ggggatcagc gaacacatgc cgcgaaccag cagattaacc ggtacgccgg
     4321 agctggaggc cgcatacaga cgatcaacca ggcctttatc gacaaggtta tttagcttca
     4381 gggtgatacc gctgggcagc ccttgctgcg cgttggcgat ttcgcggtcc accatttcat
     4441 acaacaggcg gcgggagttt tgcggcgaca ccattaaata atcaaatgtc accggacggt
     4501 atgggttttc aataaagttg aacacccgcc gtacttcgtt ggttatgcgc gcatcggcgg
     4561 tcagcaacga atagtcagta taaagacgcg cggttttttc gttaaagttc ccggtcccga
     4621 tgtgtgcgta acgcaccact tcaccgtttt ctttacgtga aatcaggaac agtttggcgt
     4681 gaattttcag ccccggcgca gagaagataa cgtgcacgcc tgcttcggtc aggcgcttcg
     4741 cccagtgaat gttggcttct tcgtcgaaac gcgcctgtaa ctcaaccacc acggtgactt
     4801 tcttaccgtt gtgtgcggcg tggatcatcg agtcgatgat gcgtgaatct ttcgccacgc
     4861 ggtaaatgtt aattttaatc gccagtacgc tcgggtcgaa cgacgcctga cgtagcagtt
     4921 ccagtacatg ctcaaaggta tgataaggat aatagagcaa cacatcgcgt tcgcgaatag
     4981 catcaaaacc attgcggaac tgggctttat caaaccaaat gtggcgtaaa cgcggcagtg
     5041 gtttgttcac cagattggct ttgccgacat tggggaaatt aataaagtct ttaaaattat
     5101 gataacgacc accggggacg atggagtcgt agcgggaaat agtcagtttt tcgcgtaaca
     5161 cttcaaccag cgcattgggc atatcgcgct gataaacaaa acgcaccggc tcagcagtta
     5221 aacgctgctt gagactggaa gacatcaact ccatcaggct ggcttccatc tcatgcacta
     5281 aatcgtattc ggcatcgcgg gtcatcttca ttgaataggc attcagcgcg tcataatcaa
     5341 agaagccttt gaaaatatca tcgaggcagt aacgcagaat gttatccaga agaatcatcg
     5401 gcttgcgtcg acgtggcgct tctggcggta aattcacaaa gcgcggcact ttatctgatg
     5461 ggatctccag cagcgcgtaa cggatggtat cgccacggat aatttccacc gccagatagg
     5521 tgtaatcatc tttcaggaac tgcactaagt cagtgtcagg attgattaaa atcggcgtaa
     5581 tgtgctgacg cagatactgc ttaaaataat gacgcagcca gttttgttga ttgacggaga
     5641 gctggcgttc attaatcagg aagatctggt tgcgcgccat ctccagcaac agctcgttgt
     5701 agaggccgtc gaattcctga tcggctttca gcacccggga ctgaattttg cccagtaaat
     5761 ggcgggaatg agagttggaa ccttgttctt cgctaataat gatgcgtcgc ttcagttcag
     5821 cgaagcggac tttatagaac tcatcaaggt tattggaata gatccccagg aaacgcatcc
     5881 tttcaatcag cgggttagat ttgtccgccg cttcctgaag cacgcgttca ttgaacgata
     5941 accaactgag ctctttttcg atgtatagct tttcctgacc cattaccact tttactccgt
     6001 tttattcacg ggacactgcc tggatattat ggcgagcaat attggcagat gtccaactat
     6061 gccagaaaag ggggaaaaaa actgctggag cttgcgattt acaggtgaat tgaaaaacgc
     6121 cagcgg
//
LOCUS       DADSZD010000215         5979 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.217, whole genome shotgun sequence.
ACCESSION   DADSZD010000215 DADSZD010000000
VERSION     DADSZD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.217"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            292..1542
                     /gene="frc"
                     /locus_tag="J1Z28_004056"
     CDS             292..1542
                     /gene="frc"
                     /locus_tag="J1Z28_004056"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBA7718831.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1596..3290
                     /gene="oxc"
                     /locus_tag="J1Z28_004057"
     CDS             1596..3290
                     /gene="oxc"
                     /locus_tag="J1Z28_004057"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBA7718832.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAAETQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3360..4304
                     /gene="yfdV"
                     /locus_tag="J1Z28_004058"
     CDS             3360..4304
                     /gene="yfdV"
                     /locus_tag="J1Z28_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBA7718833.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4378..5523
                     /gene="yfdE"
                     /locus_tag="J1Z28_004059"
     CDS             4378..5523
                     /gene="yfdE"
                     /locus_tag="J1Z28_004059"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBA7718834.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGTHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSVLCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5579..>5979)
                     /locus_tag="J1Z28_004060"
     CDS             complement(5579..>5979)
                     /locus_tag="J1Z28_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EvgS"
                     /protein_id="HBA7718835.1"
                     /translation="LKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTF
                     QHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDS
                     KPEILQLLNSVKEHIAELDQEITVFCQQND"
BASE COUNT         1583 a         1265 c         1439 g         1692 t
ORIGIN      
        1 ccttacttgt aaatcactga cacttcttac agttgtaaga ataacatcac aaatgtcgcg
       61 gctctctctg attaccgcga accaatattt attatattct tcttttcgtc ctcactactt
      121 accatgcaga aaggaattgg aagtagcgat gaaatgattt tcagcacttg atacaggaat
      181 ttaaaaatat cgagtgcagc tacgacgata ttttgcagat tatttataga ggcgttatgc
      241 cttaaataaa cgctaaatta acacatattt aattaactaa taggtattgc tatgtcaact
      301 ccacttcaag gaattaaagt tctcgatttc accggtgtgc aatctggccc atcttgtact
      361 caaatgctgg cctggtttgg cgctgacgtc attaaaattg aacgtcccgg cgttggtgac
      421 gtaacgcgcc accagctgcg agatattcct gatatcgatg cgctttactt caccatgctt
      481 aacagtaaca aacgttctat tgaattaaat accaaaacag cggaaggcaa agaggtaatg
      541 gaaaagctga tccgcgaagc tgatatctta gtcgagaact ttcatccagg ggccattgat
      601 catatgggct tcacctggga gcatattcaa gaaatcaatc cacgtctgat ttttggttcg
      661 atcaaagggt ttgatgagtg ttcgccttat gtgaatgtaa aagcctatga aaacgttgct
      721 caggcagcgg gtggcgcggc atctactaca ggtttttggg acggtccgcc gctggtaagc
      781 gctgcagcgt tgggtgacag caacaccgga atgcatttgc tgatcggttt acttgctgct
      841 ttgctgcatc gcgaaaaaac ggggcgtggg caacgagtca ccatgtcaat gcaggatgcc
      901 gtgttgaacc tttgccgcgt gaaattacgc gaccagcagc gtctcgataa attgggttat
      961 ctggaagaat acccgcagta tccgaatggc acatttggtg atgcagttcc ccgcggtggt
     1021 aatgcgggtg gtggcggtca gcctggctgg atcctgaaat gtaaaggctg ggaaaccgat
     1081 cctaacgcct atatttattt cactattcag gagcaaaact gggaaaacac ctgtaaagcc
     1141 atcggcaaac cagaatggat taccgatccg gcatacagta cagcccatgc ccgacagcca
     1201 catattttcg atatttttgc tgaaatcgaa aaatacactg tcactattga taaacatgaa
     1261 gcggtggcct atttgactca gtttgatatt ccttgtgcac cggttttaag tatgaaagaa
     1321 atttcacttg atccctcttt gcgccaaagt ggcagtgttg tcgaagtgga acaaccgttg
     1381 cgtggaaaat atctgacagt tggttgtcca atgaaattct ctgcctttac gccggatatt
     1441 aaagctgcgc cgctattagg tgaacatacc gctgctgtat tgcaggagct gggttatagc
     1501 gacgatgaaa ttgctgcaat gaagcaaaac cacgccatct gatattaacg ggggcttctg
     1561 gctcccaatt tataaaaatt tcgaggttat taatcatgtc agatcaactt caaatgacag
     1621 atggtatgca tatcatcgtt gaagcattaa aacagaataa tattgacact atttatggtg
     1681 ttgtaggtat tcctgtgacg gatatggcgc gccatgccca ggcggaaggc attcgttata
     1741 ttggttttcg tcatgagcag tcggcaggct atgccgctgc agcaagcggt tttcttaccc
     1801 aaaaaccagg gatctgcctg acagtgtctg cgccaggatt cctcaacggt ttgaccgcat
     1861 tggccaatgc aacggtaaat ggttttccga tgatcatgat tagcggctcc agcgaccgcg
     1921 cgatcgtcga tctccagcaa ggtgattatg aagagctgga ccaaatgaat gcggcaaaac
     1981 cgtatgccaa agcagcattt cgcgttaatc agccgcagga tcttggcatt gcattggcac
     2041 gcgctatccg ggtctcagta tcgggtcgtc ctggcggtgt ttatcttgat ttgccagcaa
     2101 atgtcctggc cgcgacgatg gaaaaagacg aagcgttaac cacgattgtt aaagttgaaa
     2161 atccgtcgcc agcattattg ccatgcccga agtcagtcac tagcgcaatt tcgcttttag
     2221 caaaagctga acgaccatta attattcttg gcaaaggcgc ggcgtattca caatctgatg
     2281 aacagcttcg tgaatttatt gaaagtgccc agattccatt cctgccaatg tctatggcga
     2341 aagggatcct cgaagatacg catccacttt ctgcggcagc tgcgcgttcg tttgccctgg
     2401 caaatgctga cgttgtcatg cttgttggtg cacgactgaa ttggttactg gcacacggta
     2461 aaaaaggatg ggcggcagaa acacagttta ttcaactgga tattgaaccg caggaaattg
     2521 acagcaaccg ccccattgct gtgccagtcg ttggcgatat tgcatccagt atgcaaggta
     2581 tgttggcaga attgaaacaa aacacattta cgactccact ggtgtggcgc gatattttaa
     2641 atatccacaa gcagcaaaat gcacaaaaaa tgcatgaaaa gttaagtaca gatacccaac
     2701 cattaaatta ctttaatgca ttgagtgctg tgcgcgatgt tttgcgcgag aaccaggata
     2761 tttatttagt taatgaaggg gcaaataccc tggataatgc acgaaatatt attgatatgt
     2821 ataaaccacg tcgtcgtctg gattgtggca cctggggtgt catgggcatc ggtatgggct
     2881 atgccatcgg tgctagcgtg acctctggtt ctccggttgt cgccattgaa ggtgatagtg
     2941 cttttggttt cagtgggatg gagattgaaa cgatttgtcg atataacctg ccggtgacga
     3001 tcgttatttt taataatggc ggcatctaca gaggagacgg tgttgacctc agcggcgctg
     3061 gtgcaccatc gccaacggat ctgttgcacc atgcaaggta tgacaaatta atggatgcgt
     3121 ttcgtggcgt tggctataac gtcaccacga cagatgaact tcgtcatgct ttaaccaccg
     3181 gaattcagtc gcgcaaaccg accattatta atgtggtcat cgacccagca gcaggaactg
     3241 aaagtggcca tattaccaaa cttaacccaa aacaagtcgc tggtaattaa atactgacat
     3301 cgtctcctct tgccaactta ttatgcgatg gtaaggggac ccttatcgga aatactatta
     3361 tgctaacatt ttttattggc gatttattgc ctattatcgt aatcatgctg ttgggttatt
     3421 ttagcggcag acgagaaact ttttcagagg atcaagctcg ggcatttaat aaactggtat
     3481 taaactacgc tcttcctgcg gctctatttg tttctattac cagggcaaac agggaaatga
     3541 tttttgcgga cactcgcctg acccttgtat cacttgtggt tattgtcggg tgtttctttt
     3601 tctcctggtt cggttgctac aaatttttta aacgtaccca tgcagaagca gcagtatgtg
     3661 cattaattgc aggttcacct accattggat tcctggggtt tgcagttctc gatcctattt
     3721 atggtgattc cgtatcaaca ggtttagtgg tagcaattat ttctattatt gttaacgcaa
     3781 ttactattcc tattggtctg tatttgctga atccttcctc aggagcggat ggtaagaaga
     3841 atagtaatct gagcgcatta atttctgcgg caaaggagcc agtagtatgg gcacctgttc
     3901 tggcaacgat cctggtgctg gttggggtaa aaattccggc agcatgggac ccaaccttta
     3961 atctgattgc gaaggctaac tcaggggtgg cggtattcgc tgcggggttg actctggctg
     4021 cacataaatt cgagttcagt gccgaaattg cttataacac cttcctgaag ctgattctga
     4081 tgccactggc gctgcttctc gtgggtatgg catgtcattt gaacagcgaa catctgcaga
     4141 tgatggtact ggcaggcgca ttaccgccgg cattctccgg aatcattatt gccagtcggt
     4201 ttaatgtcta cacccgcact ggtacagcgt cattggctgt gagcgtactg ggttttgtcg
     4261 tcacggctcc cttgtggatt tatgtcagtc gactggtttc ataattaatc atgttattga
     4321 ttttacataa atgccgaagt gtcttttcac cttcggcttt tttcaaggca taagcaaatg
     4381 acaaataatg aatgcaaagg gccgtttgaa ggcttattag ttatcgatat gacgcatgtc
     4441 cttaatgggc ctttcggaac tcaacttctt tgtaatatgg gcgcaagggt aattaaagtt
     4501 gagccgccag gtcatggtga tgatacccgc acatttggtc cctatgtgga tggacagtca
     4561 ctctattaca gttttattaa tcatggcaaa gagagtgtgg ttcttgattt aaagaatgat
     4621 cacgataaaa gcatatttat aaatatgctt aaacaagcgg atgtattagc tgagaatttt
     4681 cgcccaggta caatggaaaa actggggttt tcatgggaaa cgctacaaga aatcaacccg
     4741 cgccttatat atgcttcatc gtcaggtttt gggcataccg gcccgctaaa agatgctcct
     4801 gcctacgata ccatcattca ggcaatgagc gggataatga tggaaacagg ataccctgat
     4861 gctccgccag tgcgcgttgg tacctctctt gcggatctct gtggcggtgt ttatttattc
     4921 agtggaatag tgagtgcact ttatggccgc gaaaagagcc agagagggac gcatgtcgat
     4981 atagcgatgt ttgatgccac tctgagtttt ctggagcatg gactgatggc atatatcgcg
     5041 actgggaagt caccacaacg cttgggaaat cgccatccct acatggcacc ttttgatgtt
     5101 ttcgatactc aggataagcc gattacgatt tgttgtggta atgacaagct tttttctgtg
     5161 ttatgccagg cactggagct tacagaactg gttaatgatc cccgatttag cagcaatatt
     5221 ttacgcgtac aaaaccaggc tattcttaaa caatatattg agcgaacgtt aaaaacgcag
     5281 gcagctgaag tttggttagc cagaatacat gaagttggtg tgcccgtcgc gccgttatta
     5341 agtgtggctg aggccattaa tttaccacaa actcaggcga gaaatatgtt gattgaagcc
     5401 gggggaataa tgatgccggg taatccgata aaaatcagcg gctgcgcgga cccgcatgtt
     5461 atgccgggag cggcaacgct cgaccagcat ggggaacaaa ttcgccagga gttctcatca
     5521 taaatgtaag atgtgctggt aaatagctcc cacatttgaa cattgtggga gccgctattt
     5581 agtcattttg ctgacagaaa acagtaatct cctggtccag ctctgcaatg tgttctttta
     5641 cagagttcag caactgaaga atttcaggct tactgtcatc tgaaacaggt gttatttcta
     5701 actggtggct aatattaatc aacttttgca aattcaggat gttagccgca ccgtggatgc
     5761 gatgaataca ctgatggaaa gttctgttat cgccagcttc tagtgcatga aacgcagcgg
     5821 gtagatcttt atgcgtttca tgctggaaag tcatgagaat ctcctgcatc agttgtagat
     5881 cattcgccgt attattcttc agggcctcga tatcaaggtg gcgatactga ggtgcaatat
     5941 gcgcaacctg gtgtaactga cttaaatgtg ttttcagta
//
LOCUS       DADSZD010000216         5978 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.218, whole genome shotgun sequence.
ACCESSION   DADSZD010000216 DADSZD010000000
VERSION     DADSZD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.218"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..275
                     /gene="papA"
                     /locus_tag="J1Z28_004061"
     CDS             <1..275
                     /gene="papA"
                     /locus_tag="J1Z28_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P fimbria major subunit PapA"
                     /protein_id="HBA7718836.1"
                     /translation="KGTVKLAFTGPIVNGHSDELDTNGGTGTAIVVQGAGKNVVFDGS
                     EGDANTLKDGENVLHYTAVVKKSSAAGAAVTEGAFSAVANFNLTYQ"
     gene            345..932
                     /gene="papH"
                     /locus_tag="J1Z28_004062"
     CDS             345..932
                     /gene="papH"
                     /locus_tag="J1Z28_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapH"
                     /protein_id="HBA7718837.1"
                     /translation="MRLRFSVPLFFVCCAFFRGVFAGPFPPPGMTLPEYWGEENVWWD
                     GRATFHGEVVRPACTLAMEDAWQIIDMGETPVRDLQNGFSGSERKFSLRLRNCEFNSQ
                     GGNLFSDSRIRVTFDGVRGETPDKFNLSGQAKGINLQIADARGNIARAGKVMPAIPLT
                     GNEEALDYTLRIVRNGKKLEAGNYFAVLGFRVDYE"
     gene            990..3500
                     /gene="papC"
                     /locus_tag="J1Z28_004063"
     CDS             990..3500
                     /gene="papC"
                     /locus_tag="J1Z28_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial usher protein PapC"
                     /protein_id="HBA7718838.1"
                     /translation="MKDRIPFAVNNITCVILLSLFCNAASAVEFNTDVLDAADKKNID
                     FTRFSEAGYVLPGQYLLDVIVNGQSISPASLQISFVEPALSGDKAEKKLPQACLTSDM
                     VRLMGLTAESLDKVVYWHDGQCADFHGLPGVDIRPDTGAGVLRINMPQAWLEYSDATW
                     LPPSRWDDGIPGLMLDYNLNGTVSRNYQGGDSHQFSYNGTVGGNLGPWRLRADYQGSQ
                     EQSRYNGEKTTNRNFTWSRFYLFRAIPRWRANLTLGENNINSDIFRSWSYTGASLESD
                     DRMLPPRLRGYAPQITGIAETNARVVVSQQGRVLYDSMVPAGPFSIQDLDSSVRGRLD
                     VEVIEQNGRKKTFQVDTASVPYLTRPGQVRYKLVSGRSRGYGHETEGPVFATGEASWG
                     LSNQWSLYGGAVLAGDYNALAAGAGWDLGVPGTLSADITQSVARIEGERTFQGKSWRL
                     SYSKRFDNADADITFAGYRFSERNYMTMEQYLNARYRNDYSSREKEMYTVTLNKNVAD
                     WNTSFNLQYSRQTYWDIRKTDYYTVSVNRYFNVFGLQGVAVGLSASRSKYLGRDNDSA
                     YLRISVPLGTGTASYSGSMSNDRYVNMAGYTDTFNDGLDSYSLNAGLNSGGGLTSQHQ
                     INAYYSHRSPLANLSANIASLQKGYTSFGVSASGGATITGKGAALHAGGMSGGTRLLV
                     DTDGVGGVPVDGGQVVTNRWGTGVVTDISSYYRNTTSVDLKRLPDDVEATRSVVESAL
                     TEGAIGYRKFSVLKGKRLFAILRLADGSQPPFGASVTSEKGRELGMVADEGLAWLSGV
                     TPGETLSVNWDGKIQCQVNVPETAISDQQLLLPCTPQK"
     gene            3571..4305
                     /locus_tag="J1Z28_004064"
     CDS             3571..4305
                     /locus_tag="J1Z28_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7718839.1"
                     /translation="MWKLSMIRKKILMAAIPLFVISGADAAVSLDRTRAVFDGGEKSM
                     TLDISNDNKQLPYLAQAWIENENQEKIITGPVIATPPVQRLEPGAKSMVRLSTTPDIS
                     KLPQDRESLFYFNLREIPPRSEKANVLQIALQTKIKLFYRPAAIKTRPNEVWQDQLIL
                     NKVSGGYRIENPTPYYVTVIGLGGSEKQAEEGEFETVMLSPRSEQTVKSANYNTPYLS
                     YINDYGGRPVLSFICNGSRCSVKKEK"
     gene            4342..4923
                     /locus_tag="J1Z28_004065"
     CDS             4342..4923
                     /locus_tag="J1Z28_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein papJ"
                     /protein_id="HBA7718840.1"
                     /translation="MVVNKTTAVLYLIALSLSGFIHTFLRAEERGIYDDVFTADELHH
                     YRINERGGRTGSLAVSGALLSSPCTLVSNEVPLSLRPENHSASAGAPLMLRLAGCGDG
                     GALQPGKRGVAMTVSGSLVTGPGSGSALLPDRKLSGCDHLVIHDGDTFLLCRPDRRQE
                     EMLAAWRKRATQEGEYSDARSNPAMLRLSIKYE"
     gene            4933..5469
                     /locus_tag="J1Z28_004066"
     CDS             4933..5469
                     /locus_tag="J1Z28_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7718841.1"
                     /translation="MIKSTGALLLFAALSAGQAMASDVAFRGNLLDRPCHVSGDSLNK
                     HVVFKTRASRDFWYPPGRSPTESFVIRLENCHATAVGKIVTLTFKGTEEAALPGHLKV
                     TGVNAGRLGIALLDTDGSSLLKPGASHNKGQGEKVTGNSLELPFGAYVVATPEALRTK
                     SVVPGDYEATATFELTYR"
     gene            5496..>5978
                     /gene="papE"
                     /locus_tag="J1Z28_004067"
     CDS             5496..>5978
                     /gene="papE"
                     /locus_tag="J1Z28_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P fimbrial minor subunit PapE"
                     /protein_id="HBA7718842.1"
                     /translation="MKKIRGLCLPVMLGAVLMSQHVHAVDNLTFRGKLIIPACTVSNT
                     TVDWQDVEIQTLSQNGNHEKEFTVNMRCPYNLGTMKVTITATNTYNNAILVQNTSNTS
                     SDGLLVYLYNSNAGNIGTAITLGTPFTPGKITGNNADKTISLHAKLGYKGNMQNLIAG
                     P"
BASE COUNT         1516 a         1303 c         1680 g         1479 t
ORIGIN      
        1 aaaaagggac tgttaagctg gcttttactg gcccgatagt taatggacat tctgatgagc
       61 tagatacaaa tggtggtacg ggcacagcta tcgtagttca gggggcaggt aaaaacgttg
      121 tcttcgatgg ctccgaaggt gatgctaata ccctgaaaga tggtgaaaac gtgctgcatt
      181 atactgctgt tgttaagaag tcgtcagccg ctggtgccgc tgttactgaa ggtgccttct
      241 ctgccgttgc gaatttcaac ctgacctatc agtaacagta atacgataat caggtccggt
      301 aaacagcgga gcacttccgc tgtttatttc tcagggtatt tgtcatgaga ctgcgattct
      361 ctgttccgct tttctttgtt tgctgtgcat tttttcgtgg tgtttttgcc ggtccgtttc
      421 ctccgcccgg catgaccctt cctgagtact ggggagaaga aaacgtatgg tgggatggca
      481 gggctacttt tcatggtgag gttgtcagac ctgcctgtac cctggcaatg gaagacgcct
      541 ggcagattat cgatatgggg gaaaccccgg tacgggattt acagaatggt ttttccggat
      601 ctgaaagaaa attcagccta cggctcagga actgtgaatt taacagtcag ggtggaaacc
      661 ttttctctga ttcccggata agggtgactt tcgatggtgt ccggggtgaa acgccggata
      721 agtttaattt atccggtcag gcaaaaggca ttaatctgca gatagctgat gccaggggaa
      781 atattgcccg ggcagggaaa gtaatgcctg caataccatt gacgggtaat gaagaagcgc
      841 tggattacac cctcagaatt gtgagaaacg gaaaaaaact tgaagccgga aattattttg
      901 ccgtgctggg attccgggtc gattatgagt gagtcactcc ggtgagatgt ccggttattt
      961 atctttttgt gaatctggtg atgcgtggaa tgaaagacag aatacctttt gcagtcaaca
     1021 atattacctg tgtgatattg ttgtctctgt tttgtaacgc agccagtgcc gttgagttta
     1081 atacagatgt acttgacgca gcggacaaga aaaatattga cttcacccgt ttttcagaag
     1141 ccggctatgt tctgccgggg caatatcttc tggatgtgat tgttaacggg caaagtattt
     1201 ctcccgcatc gttacagatt tcatttgttg aacctgcgtt gtcaggagat aaggcagaaa
     1261 aaaaattgcc gcaggcctgt ctgacatcag atatggtcag actgatgggg ttaacagcag
     1321 aatctctgga taaagttgtt tactggcatg atggtcagtg tgcggatttt catgggttgc
     1381 cgggagtgga tattcgtcct gataccggag cgggcgtatt acgcatcaat atgccgcagg
     1441 cctggcttga gtattctgat gccacctggc tgcctccctc acgctgggac gacggcattc
     1501 ccggactgat gctggattat aacctcaacg ggacggtttc ccgtaattat cagggaggag
     1561 actctcatca gttcagttat aacgggactg tgggggggaa tctggggccc tggcgcctgc
     1621 gggctgacta tcagggaagc caggagcaga gccgctacaa cggggaaaaa acgacaaaca
     1681 gaaatttcac atggagtcgc ttttatctgt tccgtgccat tccacgatgg cgggcaaacc
     1741 tgacgctggg cgagaataat atcaactcag atatattccg gtcatggagt tatacgggag
     1801 ccagcctgga aagcgatgac cggatgctgc cgcccagact gcgaggctat gcaccgcaga
     1861 ttaccgggat tgcggagact aatgcccgtg ttgtggtgtc gcagcaggga cgggtgctgt
     1921 acgactcgat ggtccccgca gggccattca gtattcagga cctggacagt tcagttcgcg
     1981 gacgtcttga tgttgaggtt attgaacaga acggacggaa gaaaaccttt caggtcgata
     2041 cggcctcggt tccttatctg acgcgtccgg gacaggtccg gtataaactt gtctccggtc
     2101 gttcccgcgg atacgggcat gagaccgaag ggcctgtatt tgcgaccgga gaggcatcct
     2161 gggggctcag taaccagtgg tcgctgtatg gcggggctgt gcttgccggt gattataatg
     2221 cactggcagc cggtgccggc tgggacctgg gtgtgccggg gaccctttcc gctgatatca
     2281 cgcagtcagt agcccgtatt gagggagaga gaacgtttca gggaaaatcc tggcgtctga
     2341 gctactccaa acggtttgat aatgcggatg ccgacattac gttcgccggg tatcgtttct
     2401 cagagcgaaa ctatatgacc atggagcagt acctgaacgc ccgctaccgt aatgattaca
     2461 gcagtcggga aaaagagatg tataccgtta cgctgaataa aaacgtggcg gactggaaca
     2521 cctcttttaa cctgcagtac agccgtcaga catactggga catacggaaa acggactatt
     2581 atacggtgag cgtcaaccgc tactttaatg ttttcggact gcagggtgtg gcggttggat
     2641 tgtcagcctc aaggtctaaa tatctggggc gtgataacga ttctgcttac ctgcgtatat
     2701 ccgtgccgct gggaacgggg acagcgagct acagtggcag tatgagcaat gaccgttatg
     2761 tgaatatggc cggctacact gacacgttta atgacggact ggacagctac agcctgaacg
     2821 ccggccttaa cagtggcggt ggactgacat cgcaacatca gattaatgcc tattacagtc
     2881 atcgtagtcc gctggcaaat ttgtccgcga atattgcatc cctgcagaaa ggatatacgt
     2941 ctttcggcgt cagtgcttcc ggtggggcaa caattaccgg aaaaggtgcg gcgttacatg
     3001 cagggggaat gtccggtgga acacgtcttc ttgttgacac ggatggtgtg ggaggtgtac
     3061 cggttgatgg cgggcaggtg gtgacaaatc gctggggaac gggcgtggtg actgacatca
     3121 gcagttatta ccggaataca acctctgttg acctgaagcg cttaccggat gatgtggaag
     3181 caacccgttc tgttgtggaa tcggcgctga cagaaggtgc cattggttac cggaaattca
     3241 gcgtgcttaa agggaaacgt ctgtttgcaa tactgcgtct tgctgatggc tctcagcccc
     3301 cgtttggtgc cagtgtaacc agtgaaaaag gccgggagct gggcatggtg gccgacgaag
     3361 gccttgcctg gttgagtggc gtgacgccgg gggaaaccct gtcggtaaac tgggatggaa
     3421 aaatacagtg tcaggtaaat gtaccggaga cagcaatatc tgaccagcag ttattgcttc
     3481 cctgtacgcc tcagaaataa atgaaagtcc ggaatattaa cggctgatga atgcggttat
     3541 gccattttcc ggaccaggtg aaattacaga atgtggaaat taagtatgat tcgaaaaaag
     3601 attctgatgg ctgccatccc cctgtttgtt atatccgggg cagacgctgc tgtttcgctg
     3661 gacagaaccc gcgcggtgtt tgacgggggt gagaagtcaa tgacgcttga tatctccaat
     3721 gataacaaac aactgcccta tcttgctcag gcatggatag aaaatgaaaa tcaggaaaaa
     3781 attattacag ggccggttat tgccacccct ccggttcagc gccttgagcc gggtgcgaaa
     3841 agcatggtca ggctgagtac cacaccggat atcagtaaac ttcctcagga cagggaatca
     3901 ctgttttatt ttaatctccg ggaaataccg ccgaggagtg aaaaggccaa tgtactgcag
     3961 atagccttac agaccaaaat aaagcttttt tatcgcccgg cagcaattaa aaccagacca
     4021 aatgaagtat ggcaggacca gttaattctg aacaaagtca gcggtgggta tcgtattgaa
     4081 aacccaacgc cctattatgt cactgttatt ggtctgggag gaagtgaaaa gcaggcagag
     4141 gaaggtgagt ttgaaaccgt gatgctgtct ccccgttcag agcagacagt aaaatcggca
     4201 aattataata ccccttatct gtcttatatt aacgactatg gtggtcgccc ggtactgtcg
     4261 tttatctgta atggcagccg ttgctctgtg aaaaaagaga aataatgtac cgcaataacg
     4321 gttaaatgcg ggtgggatat tatggttgtg aataaaacaa cagcagtact gtatcttatt
     4381 gcactgtcgc tgagtggttt catccatact ttcctgcggg ctgaagagcg gggcatatac
     4441 gatgacgtct ttactgcaga tgagttgcat cattaccgga taaatgaacg ggggggacgc
     4501 accggaagcc tggcagtcag tggtgcactg ctgtcctcac cctgcacgct ggtgagtaat
     4561 gaggtgccgt taagcctccg gccggagaat cactctgcgt cagccggagc acctctgatg
     4621 ctgaggctgg caggatgtgg ggacggtggt gcacttcagc ccggaaaacg gggcgttgcg
     4681 atgacagtct ccggctcact ggtaaccggt cccggaagcg gaagtgcttt acttcctgac
     4741 cgtaagctat ccggctgtga ccatcttgtt atacacgatg gtgatacctt tttactgtgc
     4801 cggcctgacc ggcggcagga ggagatgctg gccgcctgga ggaaaagggc gacacaggag
     4861 ggtgagtatt ctgatgccag gagcaatccg gccatgctgc gtctgagcat aaaatatgag
     4921 taagggaatg tgatgataaa aagcacagga gctcttttat tgtttgcagc actgtctgcc
     4981 gggcaggcaa tggcctcaga tgtggcattc aggggtaatc tgcttgacag accctgccat
     5041 gtgtccggtg acagtctgaa taaacatgtc gtcttcaaaa cccgcgcgtc cagggatttc
     5101 tggtatccgc ccggacgttc gccgacagag tcgtttgtca tcaggctgga aaactgccat
     5161 gcaacagcag ttggcaaaat tgtgaccctg acctttaagg ggacggaaga ggcggccctc
     5221 ccgggccatc tgaaggtaac cggagtgaat gccggccggt taggcattgc actgctggac
     5281 accgatggca gcagtctgct gaaacccggc gcttcccata acaaaggcca gggggaaaag
     5341 gttaccggaa acagtcttga gcttcctttc ggcgcgtatg ttgtggcaac gccggaagcc
     5401 ctgcggacga agagtgtggt acccggtgat tatgaagcaa cagccacgtt tgaattgaca
     5461 tatcgttagc aaaatttttg tacaggatat ttcagatgaa aaagataaga ggtttgtgtc
     5521 ttccggtaat gctgggggca gtgttaatgt ctcagcatgt acatgcagtt gataatctga
     5581 ccttcagagg aaaactgatt attcctgcct gtactgtaag caacacaact gttgactggc
     5641 aggatgtaga gattcagacc ctgagtcaaa atggaaatca cgaaaaagag tttactgtga
     5701 atatgcggtg tccttataat ctgggaacaa tgaaggttac gataacggca acaaacactt
     5761 ataacaatgc tattttagtt cagaatacat caaacacatc ttctgatggg ttactcgttt
     5821 atctttataa cagtaatgca ggaaatattg ggactgcgat aactttaggg actccattta
     5881 cgcccggaaa aatcacaggt aataatgcag ataaaactat atcacttcat gccaaacttg
     5941 gatataaagg gaatatgcag aatttgatag ccggtcct
//
LOCUS       DADSZD010000217         5953 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.219, whole genome shotgun sequence.
ACCESSION   DADSZD010000217 DADSZD010000000
VERSION     DADSZD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.219"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            279..734
                     /locus_tag="J1Z28_004068"
     CDS             279..734
                     /locus_tag="J1Z28_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBA7718843.1"
                     /translation="MVAEGILLEGKEWLITRCEKIYFDNLEKWFGHYCHIHCQVSLGR
                     LINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKIVGVIELDDPSHERADRIIR
                     DHWLDTIMTLARVPFVRASVTKLEEIPPVWDTRDKIRPLVAATKSEPLH"
     gene            complement(827..914)
                     /locus_tag="J1Z28_004069"
     tRNA            complement(827..914)
                     /locus_tag="J1Z28_004069"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(878..880),aa:Ser,seq:gga)
     gene            1147..2085
                     /gene="ghrA"
                     /locus_tag="J1Z28_004070"
     CDS             1147..2085
                     /gene="ghrA"
                     /locus_tag="J1Z28_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBA7718844.1"
                     /translation="MDIIFYHPTLDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGLQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            2140..2877
                     /locus_tag="J1Z28_004071"
     CDS             2140..2877
                     /locus_tag="J1Z28_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBA7718845.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFAGL"
     gene            2901..3455
                     /locus_tag="J1Z28_004072"
     CDS             2901..3455
                     /locus_tag="J1Z28_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA7718846.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVAPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3557..4048
                     /locus_tag="J1Z28_004073"
     CDS             3557..4048
                     /locus_tag="J1Z28_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBA7718847.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYAITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQQIK
                     NKA"
     gene            complement(4112..4945)
                     /gene="csgG"
                     /locus_tag="J1Z28_004074"
     CDS             complement(4112..4945)
                     /gene="csgG"
                     /locus_tag="J1Z28_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBA7718848.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(4972..5388)
                     /gene="csgF"
                     /locus_tag="J1Z28_004075"
     CDS             complement(4972..5388)
                     /gene="csgF"
                     /locus_tag="J1Z28_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBA7718849.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5413..5802)
                     /gene="csgE"
                     /locus_tag="J1Z28_004076"
     CDS             complement(5413..5802)
                     /gene="csgE"
                     /locus_tag="J1Z28_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBA7718850.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(5807..>5953)
                     /locus_tag="J1Z28_004077"
     CDS             complement(5807..>5953)
                     /locus_tag="J1Z28_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBA7718851.1"
                     /translation="IGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWAND
                     NLRR"
BASE COUNT         1491 a         1374 c         1479 g         1609 t
ORIGIN      
        1 aacgttggta atgaaatata atcaacatgg ataggtgaaa attaattatg agctttatgg
       61 cttttgtaac ttgtttgaca attgcatttg tcgccggttt tgttgttgcc agaaaattga
      121 cactccgaaa agttgcctca tcggtgatca tctctaaatc gcaaccagtc ccttcatctg
      181 caactccccc tgctgctaag gcgtcgacaa ggagctatac accgcagaaa acctatcgtg
      241 attctcgttg gcaagaacga gatgagttat ttcagggaat ggttgccgaa ggcattttat
      301 tggaaggaaa agagtggtta attacgcgtt gtgagaaaat ctatttcgat aatctggaga
      361 aatggtttgg acattattgc catatccatt gccaggtctc tctggggcgc ttgatcaact
      421 ttccagaaca acccgagttt agcgaagaag aaagaaagcg tttctttgct atttataatg
      481 gcatggcaat ggattatgtg ctggtttcaa agaagaacaa taaaatagtc ggtgttattg
      541 agctggatga tccgagtcat gaaagggcag atcgcattat tcgtgaccat tggttggata
      601 cgattatgac tcttgctcgg gtgccttttg tacgagcttc tgtcactaaa ttggaggaaa
      661 tccctccggt ctgggatacc cgtgacaaaa ttcgcccatt agtcgcggcg acaaagagtg
      721 aaccgttgca ttaaccaggt atccccggtc ggggcttctc atccccccag tgtatgcaat
      781 atacgaaaaa aaagctcgta ctttcgtacg agctcttctt taaagatggc ggtgaggggg
      841 ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac
      901 tcggacacct caccaaattg tcgttcctgt cttgctggaa cgggcgctaa tttagggaaa
      961 tcatgacctg aggtcaacaa acttttttaa aaaatcgctc gattattcaa acttcaatca
     1021 atatgcagtt ttaataagcg aaatttgctt tttttgccac cgaccacgga tttgttatgc
     1081 tggtggtctt tgtagatcat aacgataagt gcgaataaat ttcgccaaca ctcttcggga
     1141 gtcagtatgg atatcatctt ttatcaccca acgctcgata cccaatggtg gattgaggca
     1201 ctgcgcaaag ctattcctca ggcaagagtc agagcatgga aaagcggaga taatgactct
     1261 gctgattatg ctttagtctg gcatcctcca gttgaaatgc tggcagggcg cgatcttaaa
     1321 gcggtgttcg cactcggggc cggtgttgat tctattttga gcaagctaca ggcacaccct
     1381 gaaatgctga agccttctgt tccacttttt cgcctggaag ataccggtat gggcgagcaa
     1441 atgcaggaat atgctgtcag tcaggtgctg cattggtttc gacgttttga cgattatcgc
     1501 atccagcaaa atagttcgca ttggcaaccg cttcctgaat atcatcggga agattttacc
     1561 atcggcattt tgggcgcagg cgtactgggc agtaaagttg cacagagtct gcaaacctgg
     1621 cgcttcccgc tgcgttgctg gagtcgaacc cgtaaatcgt ggcctggctt gcaaagtttt
     1681 gccggacggg aagaactttc tgcatttctg agccaatgcc gggtattgat taatttgtta
     1741 ccgaataccc ctgaaaccgt cggcattatt aatcaacaat tactcgaaaa attaccggat
     1801 ggcgcgtatc tcctcaacct ggcgcgtggt gttcatgtcg tggaagatga cctgctcgcg
     1861 gcgctggata gcggcaaagt taaaggcgca atgctggatg tttttaatcg tgaaccctta
     1921 ccgccggaaa gtccgctctg gcaacatcca cgcgtgacga taacaccaca tgtcgccgcg
     1981 attacccgtc ccgctgaagc tgtggattac atttcgcgca ccattgccca gctcgaaaaa
     2041 ggggagaggg tctgcgggca agtcgaccgc gcacgcggct actaataaag catcaggttt
     2101 cctgctatcc ttggcgggaa ttgaatacag gagagagtta tgtatcccgt cgaccttcat
     2161 atgcataccg ttgccagcac gcatgcatat agcacattga gtgattacat tgcccaggcc
     2221 aaacaaaagg gcattaaact ttttgcgatc accgatcatg gcccggatat ggaagatgcg
     2281 ccgcatcact ggcacttcat taacatgcgt atctggccgc gagtggttga tggggtaggg
     2341 atcctgcgcg gcatcgaagc taacattaaa aatgttgatg gtgaaattga ttgcagcggc
     2401 aaaatgtttg attcgctgga tctgattatt gccggttttc atgagccggt ttttgcgcca
     2461 catgacaaag cgaccaatac acaagcgatg atcgccacta tcgccagcgg caatgtgcat
     2521 ataataagcc atcccggaaa cccgaaatat gaaatagatg ttaaagccgt tgctgaagca
     2581 gccgcgaaac atcaggtggc gctggaaatc aataattcct cttttttaca ctcacgtaag
     2641 ggaagtgaag acaattgtcg tgcggtagcc gcagcggtac gtgacgctgg aggttgggtg
     2701 gcattaggct cggattctca caccgcgttt accatgggtg aatttgaaga gtgtcttaaa
     2761 atcctcgacg cggtagattt tccgccagag cgcattttga atgtttctcc gcgccgctta
     2821 ctgaacttcc ttgaatctcg cggtatggca ccgattgcgg aatttgcagg cctttaatta
     2881 ctagtaatgg aaatatatta atgaatgagt tttctatcct ctgtcgtgtg ctgggttcgc
     2941 tctattaccg ccaaccgcaa gatcctttac tggtgccgtt gtttaccctg attcgtgagg
     3001 ggaaactggc tgcgaactgg ccactggagc aggatgagtt actgacgcgt ttacagaaaa
     3061 gctgtgatat gacgcaagtc tctgccgatt acaatgcgtt gtttatcggc gatgaatgtg
     3121 ctgtggcgcc atatcgtagc gcatgggttg agggcgcaac ggaagcggaa gtgcgcgctt
     3181 ttctttccga acgagggatg ccactagcgg atacgccagc cgatcacatc ggcacattgc
     3241 tgcttgcggc ttcctggctg gaagatcagt caacggaaga tgagagcgaa gcactggaaa
     3301 cactgttcag tgagtatctg ttaccctggt gtggtgcgtt ccttggcaaa gtggaggccc
     3361 atgcaactac gcctttctgg cgcaccatgg caccgctaac ccgcgatgct attagtgcaa
     3421 tgtgggacga gctggaagaa gattctgaag agtaaatgtg atctgcatca ctcataactg
     3481 caacgggtaa tttgttgttg cataaaatgt gtgctcgatc tcattcatgg ccgcgttttc
     3541 tgctatcatg cgcggcatga acatacttct ctctattgca atcacaacag gcattctctc
     3601 cggtatctgg ggatgggtgg ctgtttccct gggcttacta agctgggcgg gcttcctggg
     3661 ctgtacggcc tactttgcct gcccgcaagg tgggctgaaa gggctggcga tctccgctgc
     3721 aaccctgctt agtggcgtgg tgtgggcgat ggtcattatt tacggtagtg cactggcacc
     3781 acatctggaa atactcggtt atgccataac cggtattgtc gcgtttctga tgtgtatcca
     3841 ggccaaacag ctgctgcttt catttgttcc ggggacgttt ataggcgcat gcgcgacgtt
     3901 tgccgggcag ggtgactgga aactggtgtt accttcgctg gcgcttggac tggtatttgg
     3961 ttacgcaatg aaaaacagcg gcctgtggtt ggcggcgcgt agtgcaaaga cggctcaccg
     4021 tgagcagcaa atcaaaaata aagcgtgagg ggcactcacg ctttcgctta aaccgtaaaa
     4081 tgccggatga taattccggc ttttttatct atcaggattc cggtggaacc gacatatggc
     4141 ggtatttcac cagaatgtca ttctgccgtt ctgctttatt ttgcaaatcc cacagaccac
     4201 ggtcgatacc atcattaatc aggaaaatga cccctgtttc gatagccgac atcaggcaca
     4261 acataacagg ttcgttcgag gtgtaaccca cttccccttc cagcaagcgc tggtagtcaa
     4321 taaagcggaa aaccccggcc tgaacttcat aagaaagtat cgtcttactg gtgttcaccg
     4381 aggagaggat ctcaccggta ctcacattga cgacgcgcag gttcacggca atctgatcga
     4441 gctggtattg cgtgtcggca ccgatcccaa aatatcttgc cccaaccccg cccgatttga
     4501 cgttgctttc ataaccgata atcgaacctt caaccatgat atttgccgcc gttaaagatt
     4561 gcagcgggat tcggttatta atggcaactg tgccgttttc ttgtgccgca cgaataatct
     4621 tgcgctcgtt aagcagattt tgtaagccct ggcgctccag cggtataaac cagcgagaat
     4681 ctttcaatgc cgtgaccagc attgccgtgg cgctttgcgg aacagcagtg gagaagttac
     4741 ttgccgggta gggtttaaat tgcccggttt cgtcctgaat gttgtatacc gaaacaaaga
     4801 tcttacccgt cggcgctggc agatgggtca aatctttgta gctctgagca cgaggcatta
     4861 atgtcggtct ggcggcttct ttaggcgggg cggttaagca tccgctcagt aacatgacgg
     4921 caaccaaaag aaataagcgc tgcatgatta ttttccttat gaagctgggg cttaaaaatc
     4981 ggttgagtta ttttgtaaac ccgaaacctg gatggtcgag gtttgtccgg ttttacgatc
     5041 tgtcacgttc aactgcaatt gaccatcgcg gttagcaata tcaacaatat aatcgttggt
     5101 caccatgcgg cccggtttac cggtattaat attcgacagt agcccaccca aaatttgtga
     5161 ctggatggcc tgagtaaagt tatctaacgc tgatggcgtt tcaataccaa agtcatcgtt
     5221 atagctcgga tctttataag agttttgtgc ctgggcgcta tttaataaaa aagcaccgtt
     5281 atttgggtta ccaccaaagt ttggattacg gaactggaaa gtcatggttc cagcccaact
     5341 taatggcgaa ataagcatga gtagaactac tgcatgtttg acacgcattg cagcctccga
     5401 acaatttatt atttaaaatt catcatgcgc caaatcgccc gtacttaata acgcctggtt
     5461 tatctggcga cgatttagtg cttcttcagt ttgaatcagt gcaaagacga cagttttctc
     5521 gaagtctctt ttcaacggaa ataaaaaagt ctggaaaata acgtcctgat tcaccgttat
     5581 tgtgatccag cttccccatc gtgcactggg cctttcatta atcgttaagt tacccgtata
     5641 gtcactttcc catttatcac taaaggctcg gtaaaaatca tggccaatag atgaaacagt
     5701 atggtcagtt aacaatcccg ggacttctac ctcaacggcg tgaagattcc ctgcggcgaa
     5761 cagaaattct gccgccacaa tccagcgtaa ataacgtttc atggctttat cgcctgaggt
     5821 tatcgtttgc ccaggaaacc gcttgtgtcc ggttttttac ggctatcttc ttgaaaagat
     5881 tataaagatg cgttttaacc gtattttcgc tgatgaacaa cgaacgagcg atctcgttat
     5941 tagacgcgcc gat
//
LOCUS       DADSZD010000218         5926 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.220, whole genome shotgun sequence.
ACCESSION   DADSZD010000218 DADSZD010000000
VERSION     DADSZD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.220"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            118..1221
                     /gene="ompC"
                     /locus_tag="J1Z28_004078"
     CDS             118..1221
                     /gene="ompC"
                     /locus_tag="J1Z28_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBA7718852.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1333..2388
                     /gene="apbE"
                     /locus_tag="J1Z28_004079"
     CDS             1333..2388
                     /gene="apbE"
                     /locus_tag="J1Z28_004079"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBA7718853.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGAGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2462..3526
                     /gene="ada"
                     /locus_tag="J1Z28_004080"
     CDS             2462..3526
                     /gene="ada"
                     /locus_tag="J1Z28_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBA7718854.1"
                     /translation="MKYATCLTDDQRWQSVLARDSNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPLQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRELLAKGVSVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADSALADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3526..4176
                     /gene="alkB"
                     /locus_tag="J1Z28_004081"
     CDS             3526..4176
                     /gene="alkB"
                     /locus_tag="J1Z28_004081"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBA7718855.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHHLCQR
                     AATAAGYPDFQPDACLINRYVPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKVGGHPLTTDCRYNLTFRQAGKKE"
     gene            4252..5895
                     /gene="yojI"
                     /locus_tag="J1Z28_004082"
     CDS             4252..5895
                     /gene="yojI"
                     /locus_tag="J1Z28_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBA7718856.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIKTA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1390 a         1501 c         1621 g         1414 t
ORIGIN      
        1 gtgttggatt attctgcatt tttggggaga atggacttgc cgactgatta atgagggtta
       61 atcagtatgc agtggcataa aaaagcaaat aaaggcatat aacagagggt taataacatg
      121 aaagttaaag tactgtccct cctggtccca gctctgctgg tagcaggcgc agcaaacgct
      181 gctgaagttt acaacaaaga cggcaacaaa ttagatctgt acggtaaagt agacggcctg
      241 cactatttct ctgacgacaa gtctgtagat ggcgaccaga cctacatgcg tcttggcttc
      301 aaaggtgaaa ctcaggttac tgaccagctg accggttacg gccagtggga atatcagatc
      361 cagggcaaca gcgctgaaaa cgaaaacaac tcctggaccc gtgtggcatt cgcaggtctg
      421 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttatgacgta
      481 acttcctgga ccgacgtact gccagaattc ggtggcgaca cttacggttc tgacaacttc
      541 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ccgacttctt cggtctggtt
      601 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      661 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      721 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgac
      781 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta cactggtggt
      841 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctacaacgca
      901 actcgcgtag gttccctggg ttgggcgaac aaagcacaga acttcgaagc tgttgctcag
      961 taccagttcg acttcggtct gcgtccgtcc ctggcttacc tgcagtctaa aggtaaaaac
     1021 ctgggtgtca tcaatggccg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1081 ggtgcgacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1141 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1201 ggcctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1261 ttcattgttt tcagcgtaca aacacagttt tttggtgtac tcttgcgacc gttcgcatga
     1321 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1381 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1441 cttgaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgacgccaaa
     1501 cgcagtgccg aacttaaaga aaagattcag acccaactgg acgctgacga tcagctgctt
     1561 tcgacctata aaaaagattc cgcgctgatg cgctttaacg actcgcaaag tttgtcgccg
     1621 tggccggtaa gtgaagcgat ggccgatatc gtcactacct cgctgcgcat tggcgcgaag
     1681 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggtccg
     1741 gaacaacaac cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1801 ggcttacagc acctgacggt gattaatcag tcccaccagc aatatctgca aaaagacctg
     1861 ccggatttat atgtcgatct ctctaccgtc ggtgaaggtt atgcggcgga tcacctggca
     1921 cgcttgatgg agcaggaagg gatttcccgc tatctggtat cggtgggcgg cgcgctgaac
     1981 agccgtggta tgaacggtgc aggccagccg tggcgggtag cgattcaaaa gcccaccgat
     2041 aaagaaaacg cggttcaggc cgtggtggat atcaacggtc atgggattag tacctctggc
     2101 agctaccgta actattacga actggacggc aaacgtcttt ctcatgttat cgatccgcaa
     2161 accgggcgtc ctatcgaaca caatctggta tccgtgacgg tgattgcccc gacggcgctg
     2221 gaggccgatg cctgggatac tggcttaatg gtgctcgggc cggagaaagc caaagaggtt
     2281 gttcgccggg aagggctggc ggtttatatg atcaccaaag agggcgatag ctttaaaacc
     2341 tggatgtcgc cgcagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2401 ttggtttttg tgcgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2461 tatgaaatac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2521 ctcgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2581 gtcttgccga gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2641 ggcgcttgcc gctggctttc gtccctgcaa acgttgtcag ccagacaaag ccaatcccct
     2701 gcaacatcgc cttgataaaa ttacccacgc ctgccgactt ctggaacagg aaacgcctgt
     2761 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatttgc atcggttgtt
     2821 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     2881 gcgcgaattg ctggcgaaag gggtgagcgt gactacatct attcttaacg ccggattccc
     2941 cgacagcagc agttactacc gcaaagccga cgaaacgctg ggcatgacgg ctaaacaatt
     3001 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggccg
     3061 ttgcctggtg gcagaaagtg agcgggggat ttgcgcgata ttactgggcg atgatgacgc
     3121 gacactaatc agcgagttac agcagatgtt tcccgctgcc gacagcgcgc ttgctgatct
     3181 tacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3241 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3301 cacgatacct tgcggtgaaa ccgtcagtta tcagcaactg gctaatgcca tcggcaaacc
     3361 gaaagcggta cgggccgttg ccagtgcctg tgccgccaac aagctggcta tcgttatacc
     3421 ttgtcatcgg gtggtccgtg gtgatggcac tctttccggt taccgctggg gcgtgtcgcg
     3481 caaagcgcaa ctgctgcgcc gcgaagctga aaatgaggaa aggtaatgtt ggatctgttt
     3541 gctgatgctg aaccgtggca agaaccactg gcggctggag cagtgatttt gcaccgtttt
     3601 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3661 tttcgccaga tggtcactcc aggcggatat accatgtcgg tggcgatgac caactgtggg
     3721 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3781 aataaaccgt ggcccgccat gccgcagagt tttcatcatt tatgtcaacg tgcggctacg
     3841 gcggcgggct atccagattt ccagccagat gcctgtttga tcaaccgcta cgttcctggc
     3901 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc aattgtttct
     3961 gtttctctgg gcttacccgc gatttttcaa tttggcggcc tgaaacgaaa tgatccgctc
     4021 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg ggggtgagtc gcggctgttt
     4081 tatcatggca ttcaaccgtt gaaagtgggg ggtcatccac tcaccaccga ctgccgctac
     4141 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4201 gcacgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4261 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4321 gccagtgcgg cattaggcat tggcttaatt gcttttatca atcagcgcct tatcaaaacg
     4381 gcggatacca gtctactggt gttgccggag tttctgggat tattgctgct gttgatggca
     4441 gtcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttatcgactg
     4501 cgtagtgaat ttatcaagcg gattctcgat actcatgtcg agcgcattga acaacttggt
     4561 agcgcctcgt tgctggcggg gttaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4621 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatttgtgg
     4681 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcagtcac catctggggt
     4741 ggttttatac tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aactgaagat
     4801 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4861 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     4921 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     4981 ctgggcgcaa tcggcctggt gttctggatg gcgaacagtc tcggttgggc tgataccaac
     5041 gttgccgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5101 ggcgcattac cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5161 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggctt ttcccaactg gcaaacgctg
     5221 gagctgcgta acgtgacgtt tgcttatcag gataacgcgt tttccgttgg gccgattaac
     5281 ctcaccatca aacgtggtga actgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5341 acgctggcga tgttgctgac gggcttgtac caaccacaaa gcggcgaaat cttgctggat
     5401 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5461 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5521 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5581 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5641 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5701 ttccgtcgtg agttttatca ggtgctgctg ccgctgatgc aggagatggg taaaactatt
     5761 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5821 aacgggcaac ttagcgagct gacaggcgaa gagcgcgatg ctgcttcgcg tgatgccgtt
     5881 gcccggacgg cataatatta cgacgctgac ctggcggact gctgcc
//
LOCUS       DADSZD010000219         5922 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.221, whole genome shotgun sequence.
ACCESSION   DADSZD010000219 DADSZD010000000
VERSION     DADSZD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.221"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            17..295
                     /locus_tag="J1Z28_004083"
     CDS             17..295
                     /locus_tag="J1Z28_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HBA7718857.1"
                     /translation="MLRHTLSCYRCSVKLTAANICNEHFWLLIELSGVRSEKIIMSLK
                     DYLVDGFSKKEACEKNGVSLSYFSVTLKKLSYINNISALLSEYYHGDC"
     gene            353..1621
                     /locus_tag="J1Z28_004084"
     CDS             353..1621
                     /locus_tag="J1Z28_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBA7718858.1"
                     /translation="MKNLLFSLLLLSCSTISAPFYSLVREAVMNDPAVMEARAELNSA
                     QSRTEQARSAHWPVITATGSKLLSQSHRYSYDYDSEDILPGIRGEVNIFASGAIEADV
                     RRSESEAEYYHYKVKETGEERIRSFVSLYLDALREKQSIEVLKQSLSRHNSILDDLNT
                     ISIHDTGRESEFVQADARRLMVRQQLNTRNRVLKSTLGKLSTWTINPVTEADLENPFS
                     LMTESRLLTDFTQAPQTGNPSWLASQADVESKKAALRAQELARYPRIDLTGSVTRDDR
                     QIGVNLSWDLFNRNASYGVSEKAAQIAAATGRLDSVARLIDETGRLSLITVRESRVEM
                     ETLRRQEQASAKVVDFYRLQFRVARKTLIELLNAENELYSVGLSRVQTEDQMLHGMLD
                     YLYSQGMLLKWSGVNLSDAKNNGAEGQVNK"
     gene            1618..3765
                     /locus_tag="J1Z28_004085"
     CDS             1618..3765
                     /locus_tag="J1Z28_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HBA7718859.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAEALAASTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRALEDIPSLAVPVLLLLNAQEAIVVVRIKGTGLTREYHVLQPGGLR
                     NKLSHEALMAVYSGYCWFVQPQATQDRRSDLPEYDMSGGWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHNDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNEI
                     VRRPVERERQTHYLTVKDIQGEFRFSDVSFSYKSGLPEALSGLSLSIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVALQQLDPSFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQIGEGGLGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVVTHRLQVLNIVDRIVV
                     MNEGRVMMDGSRDEILKKLSGKQQNDENRPAAS"
     gene            3825..5072
                     /locus_tag="J1Z28_004086"
     CDS             3825..5072
                     /locus_tag="J1Z28_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HBA7718860.1"
                     /translation="MTEKMERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFIIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQID
                     EKQNKYLTEAGSELVKTETDLAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKKLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5347..5808
                     /locus_tag="J1Z28_004087"
                     /pseudo
     CDS             5347..5808
                     /locus_tag="J1Z28_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1588 a         1173 c         1592 g         1569 t
ORIGIN      
        1 gaaactcgat gagcatatat tacggcacac gctctcctgt tatagatgta gtgttaaatt
       61 aactgcggct aatatttgta atgagcactt ttggttgcta atagaattat caggtgttcg
      121 cagtgaaaaa atcatcatgt ctttaaaaga ctatttggtt gatggttttt ccaaaaaaga
      181 agcgtgcgaa aagaacggtg tgtcactcag ctatttttct gtcaccttaa agaaactatc
      241 ttatataaat aatatctcag cattacttag tgaatattat catggtgatt gctagcgcag
      301 ttcattctag taattacgct aactatctag agtgttaaca agaacataag agatgaaaaa
      361 tttattgttc tcgttgttgt tactttcatg ttcaacaatt agtgcaccgt tttattcttt
      421 ggttagggag gccgtaatga atgatccagc tgttatggaa gcccgagccg agttaaattc
      481 tgcacaatcc cgcacagagc aggctcgttc tgcacattgg ccggttatca ctgctacggg
      541 aagtaaactc ctttcacaga gtcaccgtta ttcttatgat tatgactctg aagatatttt
      601 gcctggtatt cgtggtgaag tgaatatttt cgcttcagga gcaattgagg cggatgtacg
      661 tcggagtgaa tcagaagcag agtattatca ttataaagtg aaagaaacag gggaagaaag
      721 aattcgttcc tttgtatctc tatatcttga tgcactcagg gaaaaacaat ccattgaggt
      781 acttaaacag agtctttccc gacataactc gatacttgat gatcttaata ccatcagtat
      841 tcacgataca gggcgggaat ctgaatttgt acaggcagat gcacgcagat taatggtccg
      901 gcagcagctt aatactcgga acagagtgct taaaagtaca ctgggaaaac tttcgacttg
      961 gacaataaat ccggtaaccg aagccgatct tgaaaatcct ttttctctga tgacagagtc
     1021 cagattgtta actgatttta cacaggcacc tcagacagga aatccatcgt ggcttgctag
     1081 tcaggctgat gttgagagta aaaaggcggc gctgagagct caagaacttg cccggtatcc
     1141 ccggatagat ttaacgggct ctgtcacccg agatgaccgg cagataggtg tcaatctgtc
     1201 ctgggacctt tttaaccgta atgccagtta cggtgtcagt gaaaaagctg cgcagatagc
     1261 cgcagctacg ggccgactgg actctgttgc ccgactgatt gatgaaaccg ggcgattatc
     1321 tctgataacg gtcagagaaa gtcgggtgga aatggaaacg ctcagacgtc aggaacaggc
     1381 ctctgccaaa gttgtagact tttatcgtct ccagtttcgt gtggcaagaa aaacactgat
     1441 tgaattactt aatgcagaaa atgaactgta cagtgtcggg ctttcccggg ttcagacaga
     1501 ggatcagatg ctccacggaa tgctggatta tctctattcc cagggaatgc tcctgaaatg
     1561 gagcggagtg aatctttctg atgcaaaaaa taacggggct gaagggcagg taaataagtg
     1621 aagagtattt ttgaaggatt atcacaggta acggcattac tgggtacacc tgtatctgct
     1681 gaggctttgg ctgccagtac tgtccggaca gatgtatcgg ggattgattt tcgttctgtt
     1741 ggcgaatttc tccgtagcga agggtttgat aatcatcttt ccaggcgggc tctggaggat
     1801 attccttcac ttgcggttcc agtgcttctt ttactgaatg ctcaggaagc aattgttgtt
     1861 gtacgtatta agggtacagg tctgactcgg gagtatcatg tgcttcagcc cggtgggctc
     1921 cgtaataaac tgtctcacga agcgttaatg gctgtttatt ctggttactg ctggtttgtt
     1981 cagccacagg caacacagga tcgtcgttct gacctgccgg agtatgacat gagcggggga
     2041 tggttctggc gggttctgtg gcgcttcagg ccatattacg gacaggttat tcttgcaagt
     2101 tttatcatta attttcttgc actggttagc tccttatatg taatgaacgt ttatgaccgg
     2161 gtcataccta atcgatcctg ggaaacgtta tggatgctga gtatcggagt gattgcagcc
     2221 attctgtttg aatgtgctgc aaaattaatt cggagctatc tgacggatat tgcgggtaag
     2281 aaggcagacc tgattatcag ctctgccctg ctacgccggg tgatggcggt gaaaatgtcg
     2341 gacagaccag catcctccgg ttcatacgcc agcaatttgc gggaatatga gtctgttagg
     2401 gagtttatga ccagtgccac gttgctggct tttgtggatt tgccttttat cctgcttttc
     2461 atcggtgtca tcggacttgt ggggggctgg cttgctgttg ttcctcttac actggttcca
     2521 ctggtgattc tggcaggaat attactgcag cgaccgcttt cccgtcatat taatgcctcc
     2581 atgcgggaat catcgcagcg ccagggatta cttgtggagg ctctggacgg aatagaaact
     2641 ctgaagctga ataatgcggt taactgggtc cagcgtcgct gggatggata tactgccagt
     2701 gtcgcccgtt cgtccatccg ggtaaaaaat accagtaatg tcattatctg tttttccaca
     2761 gcagttcagc aactgaatac tgttctgctg gtcattctgg gaacttatct tattcataat
     2821 gatgacccac ataaccgaat aaccatgggg gcactgattg ccgcggttat tctttccggt
     2881 cgtgcgctgg caccacttgg tcagattgca tcactggcca cccgttttca gcaggcccgc
     2941 ttggccatgc agggggtgaa tgaaattgtc cgccgtccgg tggagcggga acgtcagaca
     3001 cattatctga cagtgaagga tatccaggga gagtttcgtt tcagtgatgt ctccttttct
     3061 tataagtccg gattacccga ggcattgtcc ggtctgtcac tcagtatccg acctggagag
     3121 cgggtgggta ttattggctc aatagggtgt ggcaaatcga cgctactgaa aatgatggtg
     3181 gggctgtacc gtcagaacag gggacaaatt actcttgacg gtgttgccct gcagcaactg
     3241 gatcccagtt ttgtccgaga caaagtattt cttcttgaac agaatccccg gctttttctg
     3301 ggaactctgc gtgaaaatct gcagctttcg cgtctaccgg gaagcctgtc ggatgtggat
     3361 atgattaatg cagccggacg tttcgggttt gaccgtttta tccgacatca tcctcgcgga
     3421 cttgatatgc agatagggga aggcggtctg gggctgtcag gaggacagag gcagatggtt
     3481 gccctgacga gaatggtact gggttctccc cgggtggtac tgcttgatga gcccacgaca
     3541 ggccttgatc tgcagactga gtctgttgtt ctgaaaggtc tgagccactg gagtcgggga
     3601 cgcacaatgg tggtggtgac acatcgtctg caggttctga atattgttga ccgtattgtc
     3661 gtgatgaatg aagggcgggt aatgatggac ggttcccgtg atgaaatact gaaaaaactg
     3721 tcagggaaac agcagaatga tgagaacaga ccggcagctt cctgaacctc aaccggataa
     3781 gaaaaaagag cagatatgtt taacagtttg tggaccgcag ggatatgaca gaaaaaatgg
     3841 aacggaataa agcgtcgtca gaagtggtca ggcagtcaga cctggcgctg ttgacagatg
     3901 tacaggcagc ccttcagcag gaaaaacatc atgccctttt cctgatggtg atttttctgc
     3961 tggtactggt agtgacgttt attatctggg catggaacag ccctctggat gaagtgaccc
     4021 gtggtcaggg aagtattatt cccggtagta gggagcaggt tatacagact cttgacccag
     4081 ggattctgaa aacacttgag gtcagggaag gcgatattgt tgagaaaggt caggtactgc
     4141 tgacactgga tgatacccga agctcagcca tgttgaggga aagtgaagcc cgggtgaata
     4201 acctggaggc cgtcagggca agactccgtg cagaagctta ttctgaatca ctgacatttc
     4261 cggataacgt tcctgaggat ttgcgggagc gggaaagtac ggtctatttt ctccggaaga
     4321 cggagctggc gcagagcatc gccggcctta agcagagtaa ggctctgctg gacagggaaa
     4381 tagcaatgac acgaccaatt gtacgtgaag gggcgatgtc agaagtcgaa ctgttacgta
     4441 tgcagcgcca gtcagctgaa ctgcagttgc agatagacga gaaacagaat aaatacctga
     4501 cggaagccgg ttctgagctg gtgaagacag aaacagacct tgctcaggca aaagagaata
     4561 tggctggcag agccgacccg gttgagcgtt cacgtctcag ggctcctctg agaggtatcg
     4621 taaaaaatat acgggtaaac acactaggag gggtggtttc cgcaggacag gacataatgg
     4681 aaattattcc gctggaagat cagttactga ttgaagctta tataaatcca cgggatgttg
     4741 cctatgtcag aacagggatg ccagcacttg taaaattgac tgcttatgat tatgccattt
     4801 atggtggtct tgacggagtg gtaacactgg taagtccgga tactttacgt gatcagaaac
     4861 gtccgggaga tttaaaactg gaccctaatg aagcttatta tcgggtattg gttaccacca
     4921 gtaataatta tcttacagac aggaatggaa aaaaattacc agttattccc ggaatgatag
     4981 ccagcgtaga tataaaaacc gggcgtaaaa cagtattcca gtatctgatt aaaccgataa
     5041 ccagaatgaa gcaggcactt caggaaagat aataaaaagc acttacaggg aaggtgtttt
     5101 aaatgagcgg aataatttca cttttattct gccaggatca ttgcacatgc tgtatgtgat
     5161 ctgtagctaa agataacaca agatgtttgc ctgtcagggg aaaataaaac tgaaaaacgt
     5221 aatgattgag gaggcttaga aagcgtcaaa ttaattatat gtaactagat tcgtttttat
     5281 ttaatgactc cctttagcgg ataaaacata agagcatgct ggttttatta acctgaggat
     5341 acttatatga aaatgatttt cactggcaag gtcagtggag aaaaaacagt actggcagcc
     5401 ggagcccgtc atacagtaaa ggcacaggcc ggggaacagt atggactggt tgatgaggtg
     5461 acaggtctgg ttccggatgg ggtggaagct gaccgttcag gcgatgattt aattctacgt
     5521 aaaaaggaag atagtacaga agtccggatt gaaggattct gggaagaatg ccagccgggt
     5581 gaaacacagt gtacagcagt atttaatgtt gtgggtgaaa atggccaggt aactgaagct
     5641 gtcctgaccc aggatggccc tgtactggag aattttactg cagggcaaag tggcacatta
     5701 gctggaggta tcaataacgg gattatctgg ggggggattg cttttggtgc agggctggct
     5761 gctattgcac tggctgccgg aggcggtggt ggaagcagtc gtcataagcg tacagctgag
     5821 ggtgagcaga agccggttca gcctgaaggc ggtcaggaga ctggaaaaac agatgtatca
     5881 tctacaggct cgactaagcc ggaaagtgga aactcctctg ga
//
LOCUS       DADSZD010000220         5834 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.222, whole genome shotgun sequence.
ACCESSION   DADSZD010000220 DADSZD010000000
VERSION     DADSZD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.222"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            154..687
                     /locus_tag="J1Z28_004088"
     CDS             154..687
                     /locus_tag="J1Z28_004088"
                     /inference="COORDINATES: protein motif:HMM:NF018826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA7718861.1"
                     /translation="MLNLYITHSDLSPPDCVSTENNRLTSVISVLVSLQDNNIISVTD
                     YLKKHGISHCFYFLSTGNIRFTTHNRSMITVQTKKSPVYINYRANNIWTKLVIKKHLS
                     GNIPFFRSQFTLSRAGIFKYVLDVNMKQCQYYSVQNNILHSQFIIPVSSTIAPDRRPC
                     GCSPHPVYAAKPGVHRF"
     gene            complement(844..1029)
                     /locus_tag="J1Z28_004089"
     CDS             complement(844..1029)
                     /locus_tag="J1Z28_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718862.1"
                     /translation="MRELKMKLCALMLPLVVSACVSTPPVCPPQTKPPAPPAWVMLPA
                     PDWQTPLNGIISPSESG"
     gene            complement(1548..2081)
                     /locus_tag="J1Z28_004090"
     CDS             complement(1548..2081)
                     /locus_tag="J1Z28_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA7718863.1"
                     /translation="MSKKLRYGLSAVVLALIATGASAPEILDQFLDEKEGNHTTAYRD
                     GAGTWTICRGATRVDGRPVVPGMKLTKEKCAQVNAIERDRALAWVEKNIRVPLTEPQK
                     VGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(2086..2301)
                     /locus_tag="J1Z28_004091"
     CDS             complement(2086..2301)
                     /locus_tag="J1Z28_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBA7718864.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2379..2684)
                     /locus_tag="J1Z28_004092"
     CDS             complement(2379..2684)
                     /locus_tag="J1Z28_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HBA7718865.1"
                     /translation="MTEMKKLVTADAVKSALRTEEVRGTLKESIRKTFEAQIDADVEA
                     ILDELTGPAEEASAPQAGDGENTADSPVENSSEQPETQPGGEEQPADVLQPEPATML"
     gene            complement(2708..2884)
                     /locus_tag="J1Z28_004093"
     CDS             complement(2708..2884)
                     /locus_tag="J1Z28_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HBA7718866.1"
                     /translation="MSFIQQIQLYFCTAVCALYLVSGGYKIIRNYIRKKIDAMAAEKI
                     SAGQSAEPKSTPLP"
     gene            complement(3047..4990)
                     /locus_tag="J1Z28_004094"
     CDS             complement(3047..4990)
                     /locus_tag="J1Z28_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBA7718867.1"
                     /translation="MTFKHYDVVRAASPSELAERLSQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSGDSGTVMSTVSEPEYYFVVVLAGQSNGMSYGEGLPLPDSF
                     DRPDPRIKQLARRSTVTPGGTPCKYNDIIPADHCLHDVQDMSGINHPKADLNKGQYGC
                     VSQGLHIAKKLLPYIPQNAGILLVPCCRGGSAFTSGPDGSFSEASGASADSSRWGVGK
                     PLYQDLVSRTRAALAKNPKNKLLAVVWMQGEADLASGSQQHNGLFTTMVQQFRTDLSP
                     LAAQCVSGNAATVPWICGDTTYYWKNAGTDKYEAVYGGYKGKEAQNIFFVAFLTDENG
                     TNTPTNAPTEDPDIVAIGYYGAASRTQGSFVSTQRDSHFSSWARRGIISDRLASAILL
                     HAGRTTELMGGQTVTSPDEKPSPDAPSKPSTPPADTTTMSTLFAYRASESEGQLAPQG
                     WTAGGGKAQTVDDAGASGGKAMKLTKETGKSSWYLEHDAGNGADLLGKGGLVSCRFKV
                     DGALTANQYALALYWPVSGLPQGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQ
                     NTKLGSFGAFNNEWHTLGFRFAGHNSIEVTPVIDGQDGTPFMLSQSPVGTFRADKLRV
                     TDITNGATYPVLIDSIVVEVNNA"
BASE COUNT         1420 a         1627 c         1293 g         1494 t
ORIGIN      
        1 atcaaaaata cagataacat atattacaaa aataaattag cagaaaaata atcaccctgc
       61 ctgactatgc ggaaataaca aatagacatt tccttttcag ttcattattt tctcgccgct
      121 ccataatctt cagtcagaca tcgtcttttt tacatgctta atctttacat cactcactct
      181 gatttatcgc cacccgattg tgtctccaca gagaacaacc ggcttacttc tgttatatcc
      241 gtacttgttt cattacagga taacaatatt atttccgtta ccgactatct aaaaaaacat
      301 ggcatctcgc attgttttta cttcctgtcc acagggaata tacggtttac aacgcataac
      361 cggagtatga taacagtgca gacaaaaaaa agtcctgttt atataaacta cagagcaaac
      421 aatatctgga caaaactggt tattaaaaaa catttatccg gaaatattcc atttttccgg
      481 agtcaattta ctctttcccg ggctggcata tttaaatatg ttctggatgt aaatatgaag
      541 caatgccagt attattcagt gcagaacaat attctgcatt cgcaattcat cattccggtt
      601 tcttccacca tcgcaccgga caggcgacca tgtgggtgca gtccgcatcc tgtttacgcg
      661 gcaaaacccg gtgtgcatcg tttctgatta ttcccgcaca ctcacgcaga ggagtttccc
      721 cgtcgggctg cggtctctgt taatgcggga atacggcgac aatacaacgc actgagaaaa
      781 actatttcac gcactgagta ctgatatatt cctgtgcccc ttccagctgc ttctgcatcg
      841 tcatcagccg ctctctgagg gtgaaataat accgttgagc ggcgtctgcc agtctggggc
      901 gggcagcatt acccacgcgg gcggtgccgg tggctttgtc tgtggcggac acacaggtgg
      961 cgttgacacg caggcgctta cgaccagcgg caacatcagc gcgcagagtt tcattttcag
     1021 ctctcgcatc ggctaactcc tttgtgtatt ttgcatcaag ttcagcaaca tcacgctgac
     1081 gctgctgcat gtcagtaatg gtcgcgttcg cctggctgag ttttctcttc gcatcatccc
     1141 gctgcgcttt ataagtaatg gcgttatcgc ggtaatggtc tgttgccagc cacagcgcac
     1201 cgcaggccac cagcaatgca ataatgacca cataaagaac gcggttcata tcaccaccag
     1261 cgtatctgac caataaaata accagaggcc ataatcacaa acaccagcca gataaggatg
     1321 aacttccagg tggataattt ttcagccatc actcgtatct cccgaatcag tttgctaaaa
     1381 tcaaacacac tttctccttt gacttttccg gagtctggaa atacaagaac cccgcttgcg
     1441 gccaacaaac ggggttttac ttttattcac tcacgttttg ccagttctca gtatttcgtg
     1501 ttatccactc acgctggcca ctgccatttt tcagcaaaat attctgctta tctgtcgata
     1561 ccccagcacg ccagcgcact ctcctggtca cgccgggata cctgaccgta gcagttattt
     1621 gagcggatac ggcagtctct gccaccgtcc tttatccacc agcgaatcgc ttcgcaggca
     1681 ccttttcgat cacctgcatt aattcgttta taaaacgtcg acggaaaaca cttaccggga
     1741 ccaatgttgt acggacagaa tgacgcgatc cccactttct gcggttcggt cagcggtacc
     1801 cggatgtttt tctccaccca tgccagcgcc ctgtcgcgtt caatggcatt aacctgagcg
     1861 catttttcct tcgtcagctt catgccgggg actacaggtc tgccatccac ccgggtggca
     1921 ccccggcaga tagtccaggt acctgcgcca tcacggtatg ccgtggtgtg gttaccttcc
     1981 ttttcatcca gaaactggtc aaggatttca ggcgcagaag cccctgtagc aatcagcgcc
     2041 agaacgaccg cagataaacc gtagcggagt tttttactca ccagttcatt ctccccgcgc
     2101 tgccttgcgc cggtcctctc tgattttaaa atacaggttg gtcaggtacg tcagcagacc
     2161 aaacagcaga ctccccagca cgcctattgc cgcccactga gacggggata ccctgtccag
     2221 caactgcaga aaccagtatc ccgtccccac cgcagacgtg gtgtatgaca cacctgttgt
     2281 gattttttcc atctggtaca tatccccgcc tcccgttcac cggaagcgca cgataacaaa
     2341 agaccaccgc gatatgctgg tggcctgtgc gtcatgtatc acagcatggt tgctggctcc
     2401 ggctgcagaa catcagccgg ttgttcctca cccccgggtt gtgtctccgg ctgttcgctg
     2461 ctgttttcca ccggagaatc tgctgtattc tcaccatccc ctgcctgtgg cgcagatgcc
     2521 tcctcagcag gtccggtaag ctcatccaga atcgcttcca catccgcatc aatctgagcc
     2581 tcaaacgttt tacggataga ttctttcagc gtaccgcgaa cttcttcagt gcgcagcgca
     2641 gacttcactg catcagcagt gaccagtttt ttcatttctg tcatggtttt ctcgctgtga
     2701 gatgtgatta cgggaggggc gtggactttg gctctgctga ctgaccggca ctgatttttt
     2761 cagccgccat ggcatcaatc tttttacgga tgtagttgcg gatgatttta taaccaccac
     2821 tcaccagata cagtgcacac acagccgtac agaaatacag ctgtatttgc tgaataaatg
     2881 acatataccc ttacctttaa tattgactac agaaacagtt accggttaaa aagtaactgc
     2941 gtaaacaatg gccttcttta attgagttgt tgtattcctt aatgggtttg gaatatcacc
     3001 cgccagtcaa caaattgctc tttgactggc ggttttttta ccctgattac gcgttattca
     3061 cttccaccac aatgctgtca atcagcaccg ggtatgtcgc accattagtg atatcagtca
     3121 cgcgtaactt gtctgcccta aacgtgccaa ccggagactg cgacagcatg aacggtgtcc
     3181 cgtcctgacc atcaatgacc ggcgtcacct caatactgtt gtgaccggca aaacggaaac
     3241 ccagcgtatg ccactcatta ttaaatgcgc cgaatgaccc cagcttcgtg ttctgcgccg
     3301 tgtttccttt gtgatacatc acgttaaggt ctgtggcatc gctctgtacg tagaacgatg
     3361 ccagcaggtt atgaccggca ttaccttcca gagtaacccc ttgtggcaga ccagaaaccg
     3421 gccagtacag cgccagtgcg tactgattag ccgtaagcgc accatcaacc ttaaaccggc
     3481 agctgacaag acccccttta cccagcaggt ccgcaccatt accggcatca tgctcaaggt
     3541 accaggagga tttgcctgtt tccttggtca gtttcatggc cttacccccg ctggctccgg
     3601 catcatccac ggtctgagcc ttaccgcctc cggcagtcca gccctgcggt gccagctgcc
     3661 cttcagactc agatgcccgg taagcaaaca gcgtactcat cgtcgtggtg tcagccggtg
     3721 gcgtggacgg ttttgatggt gcatcaggtg atggcttctc atccggtgat gttacggtct
     3781 gtccgcccat cagttcagtc gtgcgtcctg catggagcag aatagctgag gccagacggt
     3841 cagaaatgat gcccctgcgt gcccatgaac tgaaatggct gtcacgctgt gtcgagacaa
     3901 aactgccctg ggtacgggat gccgcaccgt aatacccgat agccacaata tccgggtctt
     3961 ccgtcggagc attcgttggc gtgttagtgc cgttttcatc cgtcaggaac gccacaaaga
     4021 aaatattctg tgcttccttg cctttgtagc caccgtatac cgcctcatat ttatcggtgc
     4081 cagcgttttt ccagtaatat gtcgtatcac cacaaatcca cggcaccgtg gcagcatttc
     4141 cactcacaca ctgcgcagca agcggagaca ggtcagttct gaactgctga accatggtcg
     4201 tgaataaacc attatgctgc tgacttcccg atgcaaggtc tgcttctccc tgcatccaga
     4261 ccactgccag aagtttgttt ttcgggttct tcgccagcgc cgctctggta cggcttacca
     4321 ggtcctgata cagaggttta ccgactcccc agcgtgaaga atcagcggaa gcaccgctgg
     4381 cctcactgaa tgaaccgtcc ggcccgctgg tgaatgctga cccgccacgg caacagggca
     4441 ccagaagtat cccggcattc tgcgggatat aaggcagcag ttttttggca atatgcagcc
     4501 cctgactcac acaaccatac tgccccttgt tcaggtcagc tttcgggtgg tttatcccgc
     4561 tcatgtcctg cacatcatgc aggcagtggt ctgccggaat gatgtcattg tatttacacg
     4621 gcgtaccgcc tggcgtgacc gtgctgcggc gtgccagttg cttaatccgt gggtccgggc
     4681 ggtcaaatga atccggtaac ggaagccctt caccatagga catgccattt gactgccccg
     4741 ccagaacaac aacaaagtaa tattccggct cactgactgt gctcattacc gtaccgctgt
     4801 cacccgacgg cttcaccaca acaggcgtgg tgacatcacc ctccgcagca atcgcctgca
     4861 tcagggtata aggcgtaata gcaaccggac tgccaaacgg ctgccagcct tccttcagtt
     4921 tttgtgacag tcgctccgca agctctgacg gtgaagccgc cctgaccaca tcataatgct
     4981 taaatgtcat tgctcctccc ggacgggata atgtgttaac taagatgaga gagcagatga
     5041 cagccccggc cacatgaaag ttctgagagg gaagtgctgc ctgctgctcc attacacaga
     5101 ttaattatct gtctggtagt attcagcggc tgattagaat acttcacatt ttgttatttt
     5161 gtgcagcccg ttaccattcg tagcgggctt ttttagtcca taaaaaagcc cctccggaga
     5221 ggggcatact agctatatat ccatttatca ttggctgcgt ggtgccgggt gcctcccggt
     5281 gagctcagcc cggtgtcact gagcccgcac attctcgatc caagttatcg tcggagataa
     5341 tacctgatac accagacgcc cctccgcaca gggggattca ccacgcgcag agaagaatac
     5401 tgaacactat tttctccagg caatatgcaa taacatgaag tgtgagatga cttacaatta
     5461 taaaaaacag cctacagaag actcctaaat aaagccatat caaaatgagt aacaggaaaa
     5521 gaatccaggg aaaaatttac gagcaggtat tatccagcaa cagttcagac gccataactt
     5581 attgccgata gctaccatca ataatttctt tcataaaaaa gcccctccga agaggggctg
     5641 acatacgcat ttgctttcat cattaatatg gcgccgggtg cctcccagtg ctccataact
     5701 gattaatatg gaacgcggtt tcattcagtt gcattcatga ataacactct aatcagtccg
     5761 ccccgccgct tagggggatt cgccatataa attgaaagta ctgaacacat ctgtcacaaa
     5821 caatatgcaa ttgt
//
LOCUS       DADSZD010000221         5767 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.223, whole genome shotgun sequence.
ACCESSION   DADSZD010000221 DADSZD010000000
VERSION     DADSZD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.223"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            7..996
                     /gene="ldhA"
                     /locus_tag="J1Z28_004095"
     CDS             7..996
                     /gene="ldhA"
                     /locus_tag="J1Z28_004095"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBA7718868.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            1106..1528
                     /gene="hslJ"
                     /locus_tag="J1Z28_004096"
     CDS             1106..1528
                     /gene="hslJ"
                     /locus_tag="J1Z28_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBA7718869.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(1525..1791)
                     /locus_tag="J1Z28_004097"
     CDS             complement(1525..1791)
                     /locus_tag="J1Z28_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBA7718870.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            2065..5589
                     /gene="nifJ"
                     /locus_tag="J1Z28_004098"
     CDS             2065..5589
                     /gene="nifJ"
                     /locus_tag="J1Z28_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBA7718871.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAASVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPDSVRIVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLNQFADKIPTELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         1449 a         1481 c         1487 g         1350 t
ORIGIN      
        1 agtcttatga aactcgccgt ttatagcaca aaacagtacg acaagaagta cctgcaacag
       61 gtgaacgagt cctttggctt tgagctggaa ttttttgact ttctgctgac ggaaaaaacc
      121 gctaaaactg ccaatggctg cgaagcggta tgtattttcg taaacgatga cggcagccgc
      181 ccggtgctgg aagagctgaa aaagcacggc gttaaatata tcgccctgcg ctgtgccggt
      241 ttcaataacg tcgaccttga cgcggcaaaa gaactggggc tcaaagtagt ccgtgttcca
      301 gcctatgatc cagaggccgt tgctgaacac gccatcggta tgatgatgac gctgaaccgc
      361 cgtattcacc gcgcatatca gcgtacccgt gacgctaact tctctctgga aggtctgacc
      421 ggctttacta tgtatggcaa aacggcaggc gttatcggta ccggtaaaat cggtgtggca
      481 atgctgcgca ttctgaaagg ttttggtatg cgtctgctgg cgttcgatcc gtatccaagt
      541 gcggcggcgc tggaactcgg tgtggagtat gtcgatctgc caaccctgtt ctctgaatca
      601 gacgttatct ctctgcactg cccgctgaca ccggaaaact accatctgtt gaacgaagcc
      661 gccttcgatc agatgaaaaa tggcgtgatg atcgtcaata ccagtcgcgg tgcattgatt
      721 gattctcagg cggcaattga agcgctgaaa aatcagaaaa ttggttcgtt gggtatggac
      781 gtgtatgaga acgaacgcga tctgttcttt gaagataaat ccaacgacgt gatccaggat
      841 gacgtattcc gtcgcttgtc tgcctgccac aacgtgctgt ttaccgggca ccaggcattc
      901 ctgacagcag aagctctgac cagtatttct cagactacgc tgcaaaactt aagcaatctg
      961 gaaaaaggcg aaacctgccc gaacgaactg gtttaatctt cccgctcccc tgcattccag
     1021 gggagcttat tcagataatc cccaaagacc tttcatcctc tattcttaaa atagtcctga
     1081 atcagaaaca gtaatgagaa ccacaatgaa gaaagtagcc gcgcttgttg cgctaagcct
     1141 gctgatggcg ggatgtgtaa gtagtgacaa aattgctgta acgccagaac agctacagca
     1201 tcatcgcttt gtgctggaaa gcgtaaacgg taagcccgtg accagcgata aaaatccgcc
     1261 agaaatcagc tttggtgaaa agatgatgat ttccggcagc atgtgtaacc gctttagcgg
     1321 tgaaggcaaa ctgtctaatg gtgaactgac cgccaaaggg ctggcaatga cccgtatgat
     1381 gtgcgctaac ccgcagctta atgaactcga taacaccatt agcgaaatgc tgaaagaagg
     1441 tgcacaagtg gatctgaccg cgaaccagtt aacgctggcg accgcgaaac agacattaac
     1501 ttataagctg gcggatttaa tgaattaata gctgccacag ctcccggcgg caagtgactg
     1561 ttcgctacag cgtttgccgt tgggtaatgc acacatccca atcgccgtac catccagttg
     1621 gcgggcaaca gaaagcgaac cgccgatcat tgcacaattt gcttctccac tactggacat
     1681 cgacgctttt aaacctggcg ctacgtgcgc cgcagtcgcc tgctgaacag gttcactact
     1741 acacgccgac aacaataaag cggcacaccc tacccaaaac gctgctcgca tcttttcttc
     1801 ctctgatctt caagccaaac gacaccgcca taaataatag gcagcacaga gggcggcgtc
     1861 gagagctgtc ctgcgcgttg ccccgcattt ttactttttt atggctattt ttttgccctc
     1921 tgtttgatca aaacattcat tacgctgatg tgggggacac aaaagcgaaa atgcagaaga
     1981 aagccatttg ctaaaattga acgattacta ctgggcgcgc agcaatttcg tgcgcccctc
     2041 atttgcgcaa tgtaagggtg tcatatgatt actattgacg gtaatggcgc ggttgcttcg
     2101 gtggcgtttc gcaccagtga agttatcgcc atctacccta ttacccccag ttccacgatg
     2161 gcagaacagg ctgatgcatg ggccggaaac ggcttgaaaa acgtttgggg agacacacca
     2221 cgcgtggttg aaatgcagtc ggaagcgggt gctatcgcta ccgtgcatgg tgctttgcaa
     2281 actggtgccc tctcaacatc gtttacgtca tcgcagggtt tgctgctgat gatcccaacg
     2341 ctgtacaaac tggcaggcga actgacgccg tttgtcctgc atgttgcggc acgtaccgtt
     2401 gccacacatg cactctctat ttttggcgat cattccgacg ttatggcggt gcgccagacg
     2461 ggttgcgcga tgttgtgtgc agcaagcgtc caggaagcgc aagactttgc tctcatttcg
     2521 cacatcgcga cgctgaaaag ccgcgtgcca tttattcatt tctttgatgg tttccgcaca
     2581 tctcacgaaa tcaataaaat tgtcccgctg gccgatgaca cgattcttga actgatgccg
     2641 caggcagaaa ttgatgctca tcgcgcccgg gcactcaacc cggaacatcc ggtgatccgc
     2701 ggtacgtccg ccaatcctga cacttatttc cagtctcgcg aagccaccaa cccatggtac
     2761 aacgcggtct atgaccatgt tgaacaggcg atgaatgatt tcgctgccgc gacaggtcgt
     2821 cagtatcagc cgtttgagta ttacgggcat ccgcaagcgg aacgggtaat tatcctgatg
     2881 ggctcagcca ttggcacatg tgaagaagtg gttgatgaat tgctgacccg tggcgaaaaa
     2941 gtcggcgtgc tgaaagttcg cctgtaccgc cccttctccg ccaaacactt gctgcaagca
     3001 ttgccggact ctgttcggat agtggcggtg ctggatcgca ccaaagagcc aggtgcgcaa
     3061 gcggaacccc tgtacctgga tgtgatgacc gcactggcag aagcctttaa taatggcgag
     3121 cgtgaaaccc tgccccgtgt cattggtggg cgctatggtc tttcatccaa agaatttggc
     3181 ccggactgtg tactggcggt atttgccgag ctcaacgcgg ctaaaccgaa agcgcgcttt
     3241 acggttggta tttacgatga tgtaaccaat ctgtcactgc cgttgccgga aaacacccta
     3301 ccaaactcgg cgaaactgga agccttgttt tatggccttg gtagtgatgg cagcgtttcc
     3361 gcgaccaaaa acaatatcaa aattatcggt aattccacgc cgtggtacgc acagggctat
     3421 tttgtttacg actccaaaaa ggcgggcggc ctgacggttt ctcaccttcg cgtgagcgag
     3481 cagccgattc gctccgctta tctcatttcc caggctgatt ttgttggctg ccaccagttg
     3541 cagtttatcg acaaatatca gatggctgaa cgtttaaaac ctggcggcat tttcctgctc
     3601 aacacgccgt acagcgcaga tgaagtatgg tcgcgcttgc cgcaggaagt tcaggccgtg
     3661 ctaaaccaga aaaaagcgcg tttctatgtg attaacgcgg cgaaaattgc ccgcgaatgt
     3721 ggcctggcgg cccgtattaa tactgtcatg cagatggcat ttttccattt gacacaaatt
     3781 ctgcctggcg atagcgccct ggccgaattg cagggcgcga ttgccaaaag ctacagcagc
     3841 aaaggccagg atctggtgga acgcaactgg caggctctgg cgctggcgcg tgaatccgta
     3901 gaagaagttc cgctgcagcc ggttaatccg cacagcgcca atcgaccgcc tgtggtttcc
     3961 gatgccgctc ctgatttcgt gaaaaccgta accgctgcga tgctcgccgg gcttggcgac
     4021 gccctccccg tttcggcgct gccgccagac ggcacctggc cgatgggcac cacgcgctgg
     4081 gaaaaacgca atatcgccga agagatcccc atctggaaag aggaactctg tacccaatgt
     4141 aaccactgtg tcgccgcttg cccacactca gctattcgcg caaaagtggt accgcctgaa
     4201 gcgatggaaa acgcccctgc cagcctgcat tcgctggatg tgaaatcgcg tgatatgcgc
     4261 gggcagaaat atgtcttgca ggtggctccg gaagattgca ccggttgtaa cctgtgcgtc
     4321 gaagtttgcc cggcgaaaga ccgtcagaat ccagagatta aagccatcaa tatgatgtcg
     4381 cgcctggaac atgtcgaaga agagaaaatc aattacgatt tcttcctcaa cctgccagaa
     4441 atcgaccgta gcaaactgga acgtattgat attcgtacat cgcagctgat tacaccgctg
     4501 tttgaatatt caggtgcttg ctccggttgt ggcgagacgc cgtatattaa attgctgact
     4561 cagctctatg gcgaccggat gttgatcgct aatgccactg gctgttcttc aatatacggc
     4621 ggtaacctgc cttcaacacc atataccacc gatgccaacg gtcgtggacc ggcatgggcg
     4681 aactcgctgt ttgaagataa cgccgaattt ggccttggtt tccgcctgac ggtcgatcaa
     4741 caccgtgtcc gcgtgctgcg tttgctcaat caatttgccg ataaaatccc gacggaatta
     4801 ctgactgcgt tgaaatcaga cgccacgcca gaggttcgtc gtgaacaggt tgcagcttta
     4861 cgccagcaac tcaacgatgt tgccgaagca catgaactgc tgcgtgacgc agatgcactg
     4921 gtggaaaaat caatctggct gattggtggc gatggctggg cttacgatat cggctttggc
     4981 ggtctggatc atgtattgag tttgacggaa aacgtcaaca ttctggtgct ggatacgcaa
     5041 tgctattcca acaccggtgg tcaggcatcg aaagcgacac cgctgggtgc agtaactaag
     5101 tttggcgagc atggcaaacg taaagcgcgt aaagatcttg gcgtcagtat gatgatgtac
     5161 ggtcatgttt atgtggcgca gatttctctc ggcgcgcagt tgaaccagac ggtgaaagcg
     5221 attcaggaag cggaagcata tccggggcca tcgctgatca tcgcttatag cccatgtgaa
     5281 gagcatggtt acgatctggc actcagccac gatcagatgc gccaactcac tgctaccggc
     5341 ttctggccgt tatatcgctt cgatcctcgt cgtgccgatg aaggcaaact gccgctggcg
     5401 ctggattcac gcccgccgtc agaagcactg gaagaaacgt tacttcacga gcagcgtttc
     5461 cgtcgtctga attcgcagca gccagaagtg gcagaacagt tatggaaaga tgctgcagct
     5521 gatttgcaaa aacgctatga cttcctggca caaatggccg gaaaagcgga aaaaagcaac
     5581 accgattaaa tgctctggat aaggattatc caattctaaa aaaaagcccg gacgactgtt
     5641 cgggcttgtc ttcttatgtg ttggaaaatc agtagcaatg caatggccca acagaaaata
     5701 aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa attaaaagta
     5761 taatttt
//
LOCUS       DADSZD010000222         5711 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.224, whole genome shotgun sequence.
ACCESSION   DADSZD010000222 DADSZD010000000
VERSION     DADSZD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.224"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            85..1419
                     /locus_tag="J1Z28_004099"
     CDS             85..1419
                     /locus_tag="J1Z28_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBA7718872.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            1472..1924
                     /locus_tag="J1Z28_004100"
     CDS             1472..1924
                     /locus_tag="J1Z28_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBA7718873.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2135..3325
                     /gene="nepI"
                     /locus_tag="J1Z28_004101"
     CDS             2135..3325
                     /gene="nepI"
                     /locus_tag="J1Z28_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBA7718874.1"
                     /translation="MSEFIAENRGANAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAAMGVLCIFWIIKSLPSLPGESSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFVGTSLSSFIL
                     KRSVKLALAGAPFVLALSALVLTLWGSDKIVATGVAIIWGLTFALIPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYVLDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3366..3659)
                     /locus_tag="J1Z28_004102"
     CDS             complement(3366..3659)
                     /locus_tag="J1Z28_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718875.1"
                     /translation="MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3881..4699
                     /gene="nlpA"
                     /locus_tag="J1Z28_004103"
     CDS             3881..4699
                     /gene="nlpA"
                     /locus_tag="J1Z28_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBA7718876.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4703..5626)
                     /locus_tag="J1Z28_004104"
     CDS             complement(4703..5626)
                     /locus_tag="J1Z28_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBA7718877.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLLLLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
BASE COUNT         1348 a         1288 c         1485 g         1590 t
ORIGIN      
        1 tgaaatgtga taattatcac tgaaccgttg ttgtcatttg ttttacaaaa gcaagggatt
       61 aaatctcctc caatggacaa aaaaatgaat aatgacaata ccgattacgt gagtaatgaa
      121 tcagggacgc tttcgcgatt atttaaacta cctcagcatg ggaccaccgt ccgcacagaa
      181 ttgattgcgg ggatgaccac ttttttaacc atggtgtaca tcgtttttgt gaacccgcaa
      241 atcctcggcg cggcacaaat ggacccgaaa gtggtgtttg ttaccacctg tttgattgcc
      301 ggtatcggca gtattgcgat gggggtattt gctaacttac ccgtggcact ggctccggca
      361 atgggcctga acgccttctt tgccttcgtg gtcgtggggg cgatgggtat ctcctggcag
      421 accgggatgg gcgcgatatt ctggggcgca gttgggctat ttttgctcac gctgtttcgt
      481 atccggtact ggatgatctc caacattcca ttaagtttac gtattggtat caccagcgga
      541 atcggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
      601 gacaccctgg tgatgattgg tgatttaagt tctcacggcg tgttgttagg tattttaggg
      661 ttttttatta taactgtgtt gtcatcacgt cattttcatg ccgcggtgct tgtttctatt
      721 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      781 attccgcctg atattagcgg cgtcattggt gaagtggatt tgagcggtgc gttatcactt
      841 gaactcgccg gtatcatttt ctcctttatg ctgatcaact tatttgattc atcagggaca
      901 ttaattggtg tgactgataa agctggctta atagatagta acggtaaatt ccccaatatg
      961 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggtacctcg
     1021 tctgttaccg cctatattga aagtacttca ggtgtggctg tcggtggtcg cacggggctg
     1081 acggctgtcg tagtcggcgt tatgttcctg ttagttatgt tcttctcacc gctggtggcg
     1141 atggttccgc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttca
     1201 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
     1261 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactaggctt tatgtcgtac
     1321 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
     1381 gcaactctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
     1441 tatcatgagt tctgattacg caggagaact catgatctgg ataatgctcg ccacgctggc
     1501 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1561 tctcagcgat cggctgaaca tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1621 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1681 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1741 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt gccgcgccga ttactgatgc
     1801 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatatcaa
     1861 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1921 ttaaaaccgt ttcgtcttat cagcctacag accgcgcctg acctggtggg tggataaggc
     1981 gttcacaccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     2041 caacatcgta aaagttgcaa aaacgttaaa tgcatcacac atttcaatgt tagactggtc
     2101 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ctgaaaaccg
     2161 cggcgcgaat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgcgt
     2221 cgcctgtctg attatcgttg agtttttgcc tgtcagtttg ttgacgccaa tggcccagga
     2281 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcgat
     2341 gtttgccagt ttgtttatta cccagaccat tcaggccact gaccgccgct acgttgtcat
     2401 tctgttcgcc gttttgctga cgctttcctg tttgctggtt tcctttgcca actcattcag
     2461 tctgctgtta atcggtcgcg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     2521 ggcgtcgctg accatgcgtc tggtaccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2581 cttcggcgcg gtttctattg cgctggtgat tgctgcgccg ttgggcagtt ttttaggcga
     2641 gcttatcggt tggcgcaatg tctttaatgc ggcggcggcg atgggcgtgc tgtgtatttt
     2701 ctggattatc aaatcactgc cttcgctgcc aggtgaatct tcgcatcaga aacaaaatac
     2761 tttccgctta ttacaacgtc cgggagtgat ggccgggatg atcgccatct ttatgtcctt
     2821 tgccggacag tttgcttttt tcacttatat tcgcccagtg tatatgaatc tggcgggatt
     2881 cggtgttgat ggcttaaccc tggtgctgct gagttttggt atcgccagct ttgtcgggac
     2941 atcgctttcg tcgtttattc tcaaacgttc ggtgaaactg gccttagcgg gcgcaccgtt
     3001 tgtgctcgcc ctgagcgcgc tggtactgac gctgtgggga agcgataaaa ttgttgctac
     3061 tggtgtggcg attatctggg ggctgacctt tgctttaatt cccgttggtt ggtcaacgtg
     3121 gatcactcgc tcattggccg atcaggcaga aaaggctgga tctattcagg tggccgttat
     3181 tcagcttgct aacacctgcg gtgcggcaat cggcggttat gtgttggata acattggtct
     3241 gacttcgccg ctgatgttgt ccggcacatt gatgttgctg actgcattgt tggtgactgc
     3301 aaaggtgaaa atgaagaaat cctgatatca ggtaaagaag cgttggtcag tggttgttgc
     3361 cgttcttaca tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta
     3421 ttacgttcca tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac
     3481 attaactttt cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa
     3541 tcttgcaata ccgtggtttt ctcttttgcc gactgtagag aactgaatgg cgactcggcc
     3601 tgagaaatgg ctggaaaaat caggaatacg gtaatcatga gtagcatcgt tggcttcata
     3661 gcggcaacct tctgcaaatg tgcatcgaat gaagcataag tgtagtttgc ttttgccgct
     3721 ctcatcatgc tgtacgaaag gtgtttgcgt ggtcagtaag aagtgccaga ctttatattc
     3781 cacttttatt cctttttatt cttatcgata gcgtttcgtt ttttaaactg cagcgacctt
     3841 accgctatag tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca
     3901 tctacgggcg ggggccgcat tattactggc aggagttctg ctggcaggtt gcgaccagag
     3961 cagcagcgat gaaaagcata ttaaagttgg cgttattaat ggcgcagaac aagatgtcgc
     4021 ggaagtcgct aaaaaggtag caaaagagaa atatggtctc gatgttgaac tggtaggatt
     4081 tagcggttcg ttgctaccga acgatgcgac aaatcatggc gagcttgatg cgaacgtctt
     4141 ccagcatcga ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcggt
     4201 gggaaatacg tttgtcttcc ccatggcggg ctattccaaa aaaatcaaaa cagtggcgca
     4261 aataaaagaa ggtgcgacag tggcgatccc caacgacccg actaaccttg gtcgcgcgct
     4321 tttactgctg caaaaagaga agctaattac tctgaaagaa ggaaaagggt tattacctac
     4381 cgcgttggat ataaccgata atccacgcca tttgcagata atggagctgg aaggggcaca
     4441 gttgccacgt gtactggacg acccgaaagt ggatgtagcg attatcagca ccacttacat
     4501 ccagcaaaca ggactttctc cggttaacga cagcgtattt attgaagata aaaattcgcc
     4561 gtatgtgaat attctggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt
     4621 tctgcaatct tatcaatcac ccgaagtcgc taaagcggca gaaaccatct ttaacggtgg
     4681 cgcggtgcct ggctggtaat tctcactcat gccgcgccgg acgatttatt tttctggcgc
     4741 ggcggcggga atccattgaa atcaaaatca ccgatgacag aatcagcagc gttcccagcc
     4801 agtccggcaa tgtgaaggtg atccccagca acaacaaaga gagcagcgcg ctacttaatg
     4861 gttctgcaca gcttaaaatg ctggcttttg gaccgccaat taattgtgct cctttcaggt
     4921 acagactaaa tgtcagggac gtaccaatga ccaccaaata aaaaaacgcc agaatcaaac
     4981 tgccgttaac gacaaagttt gtcccttgtc tggcataaaa gggcagcagg agcagaccgc
     5041 caatgagcat actccagccg acgaccggta acgtgccata gcgggcaatc agcgttgagg
     5101 gataggtggt atagaatgca gcagcaaatg ccgaggcaat cccccagaat aacgcggcag
     5161 gagagatcga taatgacgtc ggattaccgt gtgtcaccaa taaaaaagtc ccgatcagcg
     5221 atgtcaaaat agcgcaaaaa accaggatgc cggggcggga tttacgcacc agtgagaacc
     5281 aggcgacgat aatagtcggt gagaggaatt gcagcaccgt tgccgtggct gcgttcgatt
     5341 tttcgatggt tagcaaaaaa gtgagctgta cggttagcgc gccaaccacg gaaaaaatca
     5401 gtaggctaat ggcatctttg tgattgttaa taatagaaaa gattttatca ccatgaacaa
     5461 atgatagcgt cagcagaatc aaaccggcga atatcaaacg cgtcatagtc aaaaactgcg
     5521 acgacatctg gctttgctcc atgatgtatt gcgcgcagac ccctgaactt ccccacaaca
     5581 cggcggcaat cagaacgttc agcatcccct ttctggtgga acccattttt ccctctgcat
     5641 actgttttgt cttttcatca atatacgtta aggataccac taccgatgac ggtgagggag
     5701 tagagtaatc a
//
LOCUS       DADSZD010000223         5639 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.225, whole genome shotgun sequence.
ACCESSION   DADSZD010000223 DADSZD010000000
VERSION     DADSZD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.225"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            131..544
                     /locus_tag="J1Z28_004105"
     CDS             131..544
                     /locus_tag="J1Z28_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017891459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718878.1"
                     /translation="MNIRTVLFLCSSLTATAAQAVTPEQQWGDWYGYINAMEFEISTD
                     NATGERLTLTCSDEHMTLSYSVPAKDYRASATGISINATSYATGETAFIALKNTSDQQ
                     QIEITLKDKPLPGTFKTAGLREALNDLSWQDCISH"
     gene            630..1469
                     /locus_tag="J1Z28_004106"
     CDS             630..1469
                     /locus_tag="J1Z28_004106"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718879.1"
                     /translation="MKALISRLTVISALLFPALSSAVVIPGNIHPGRYTFFLTEEGGE
                     MLRDMNDAAVSVKLNNKAGFTVEAQRMAAAANISPLLYAASPLEGNFIRYDSKPVKAL
                     TCLITSRNMPGTNKNYSWEETYCLDETGAAYIGTQGWPSRTIFDKRCGVGASDCPVYL
                     SLKNDPPTLRNDSAQYVPYSSAHRSETVPNKTQNKVPADEIVAEQDGYAVHFSRSNNC
                     SDSRLTVKNISTGKTAAITGVNGCTFNPKKNAVVIYPVNEYVSVYSGTTNTSEEIATA
                     DLP"
     gene            2143..2967
                     /locus_tag="J1Z28_004107"
     CDS             2143..2967
                     /locus_tag="J1Z28_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA7718880.1"
                     /translation="MTRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSEKHESRSDR
                     YTYIPTITILESLQREGFEPFFACQTKVRDQSKREHTKHMLRLRRAGQLTGHQVPEII
                     LLNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGAF
                     DRVDEKRDAMQSLALPAPARNALANAALKYRFGEDHQPVTVSQLLTPRRREDCSDDLW
                     TVYQRVQENLMKGGLSGRTAQGKSSRTRAVTGIDGDVKLNRALWVMAENMLAFFER"
     gene            2998..3441
                     /locus_tag="J1Z28_004108"
     CDS             2998..3441
                     /locus_tag="J1Z28_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA7718881.1"
                     /translation="MDTHNPEFAEQAAITASVVPDELRIGFWPQHFGSIPQWITLEPR
                     IFAWMDRLCTDYHGGIWNFSTLSNGGAFMYPESEDDEKWALFNSMNGNGAELTSEAAG
                     IVACLMTYSHHACRTECDATTEHYYRLRDFALNHPECNSIMHLID"
     gene            3454..3996
                     /gene="radC"
                     /locus_tag="J1Z28_004109"
     CDS             3454..3996
                     /gene="radC"
                     /locus_tag="J1Z28_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBA7718882.1"
                     /translation="MSTALSPLSSQPELFPLAVMAQHGCLLPATSGLTPYAQRTIRRA
                     INLLDKYLRQPGISFTSSTAARDWLRLQLAGQEREVFMVLYLDNQHRLLESETLFAGS
                     VNHVQVHPREVVKSALRLNAAAAVLAHNHPSGDPEPSQCDRNITGRLKEALALVDVNT
                     LDHLVIGSEGIVSFAERGWI"
     gene            3993..4214
                     /locus_tag="J1Z28_004110"
     CDS             3993..4214
                     /locus_tag="J1Z28_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA7718883.1"
                     /translation="MKIISKRQAMTLYRQHPGSRLFRFSTGKYKWSGSICHYAGREVE
                     DIRGVLAVFAERRQGRNGPYVILRSVTLN"
     gene            4240..4605
                     /locus_tag="J1Z28_004111"
     CDS             4240..4605
                     /locus_tag="J1Z28_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBA7718884.1"
                     /translation="MNNHSESGTKPENPACQQWGLKRTITPCFGARLVQEGNRVHFLA
                     DRAGFNGTFSDNDALRLDQAFPLILKQLELMLTSGELSHLHQHCITLYHNGLTCEADT
                     LGSCGYVYIAIYPDQPEPH"
     gene            4643..4972
                     /locus_tag="J1Z28_004112"
     CDS             4643..4972
                     /locus_tag="J1Z28_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBA7718885.1"
                     /translation="MQTLSSHPTRATQPNLSPVETWQRLLTHLLSQHYGLTLNDTPFS
                     NETTIREHIDAGVSLSDAVNFLVEKYGLVRIDRKGFSWQEQTPYISIVDILQARRSTG
                     LLKTNVK"
BASE COUNT         1506 a         1515 c         1310 g         1308 t
ORIGIN      
        1 tcaatttctg ataaccgatc tgtattaacg attatcccaa cagaccacga atgcatacga
       61 tcaggctgct tcctgcatgc cgggaagtgg cttttcacca tataaatcac acagtaaagg
      121 aaagatttaa ttgaacattc gcacagttct ttttctctgc tcatcgctga cagcgactgc
      181 tgcacaggcc gttactcccg aacaacagtg gggtgactgg tatggctaca tcaatgccat
      241 ggagtttgaa attagcaccg acaatgccac cggagagcgc ctgacgctga cctgctcaga
      301 cgagcatatg accctctcct acagtgtacc tgcaaaggac tatcgggcct cagccaccgg
      361 tataagcatt aacgcaacct catacgctac aggcgaaacg gcctttattg cgctcaaaaa
      421 taccagtgat cagcagcaga tagagatcac gttgaaagat aagccccttc cgggaacctt
      481 taaaaccgcg gggttgcgcg aagcgttgaa tgacctgagc tggcaggact gtatcagcca
      541 ttaaggctca tgagaggccc tggtcataaa gactcactgg gttaacgatg atttcgacaa
      601 cctcacaact cacgattcag gatgtaaaaa tgaaggcact aatttcacga ctcaccgtca
      661 tttcagctct gctatttccc gcgttaagct ctgcagtggt tatccccgga aatatacacc
      721 caggacgtta cacgttcttt ctgacagagg aaggtggcga aatgctacgc gatatgaacg
      781 atgctgcagt gagcgtcaag ctcaacaata aagcaggctt cactgttgag gcgcaaagaa
      841 tggccgctgc agcgaatatt tcgcctttgc tatatgccgc atcaccactt gaaggaaact
      901 ttatccgcta cgacagtaaa cccgttaagg ccctgacctg ccttatcact tcccgtaata
      961 tgccgggcac caacaagaat tattcatggg aggaaaccta ctgtctggat gaaaccggtg
     1021 cagcttacat tggcacccaa ggatggccgt cacgcacaat attcgacaaa cgctgtgggg
     1081 tgggtgcatc tgactgcccg gtctatctga gcctgaaaaa tgatccaccc accctgcgca
     1141 acgactccgc acagtatgtg ccatacagca gcgctcaccg ttctgagacg gtgcccaata
     1201 aaactcagaa taaagtaccc gctgacgaaa ttgtcgctga acaggatgga tatgcggttc
     1261 acttttcccg aagcaacaac tgttctgaca gcagactcac cgttaaaaat atcagcacag
     1321 ggaaaactgc tgcgatcaca ggtgtcaacg ggtgcacatt taatccgaaa aagaacgctg
     1381 tggttatcta tccagttaat gaatacgtga gcgtttacag cggtactacc aatacctctg
     1441 aagaaatagc gactgccgat ctcccttaat cactcacctg acccggtata gacattcttc
     1501 tataccggtc attatggaat tcccatgaaa tctaatcacc ttcaagatga taaaattccc
     1561 ctgaccacat cacccggtct tacctggcct gtagtcttct gcgtcgccgg aataacactt
     1621 ctgcgaagca agtctgtcgt gtgtcagacg ataggggcca gcctgaccac actgtgcctg
     1681 gtaacgctga taaaaaaact ctccactctg gtctgagggg ttcgctacct ggcaaagcca
     1741 aatgtgcacc tgagactgga ccagaagaca ctccccccct gataaaaaaa cctggcaaaa
     1801 cataccaaca attagtcagg cgtattgtta tccatgatct ggcggtgctg gcagaggcaa
     1861 ggctcaggga tgacgcattt gctttttctg tatgcagtaa agcataccgg cattttccct
     1921 cattcatccg acgaaggtat ccccaggaac agtacctccg ttttttgagg cagcatctgc
     1981 ctattatcct tgtacgttta cgtcaaatct gggattacac gtcccccact ttactcccgt
     2041 ctcaagacaa tgagacatag ccacctgctt cacccaggac attttatctt tcgaccacgc
     2101 cactctccgg agtggcattt ctgtctattt aaggaaccag ttatgacccg tttagccagc
     2161 cgttttggcc gtataaacca gatacgccgt gaccggcctc tgacccacga agagctgatg
     2221 agccatgtcc ccagcgtgtt cggtagtgag aaacatgaat ctcgttcaga ccgttacacc
     2281 tacatcccga ccatcaccat ccttgaaagc ctgcagcgtg agggctttga gccgttcttt
     2341 gcctgccaga caaaggttcg cgaccagagc aagcgggagc acaccaaaca catgcttcgc
     2401 ctgcgtcgtg ccggtcaact gaccgggcat caagtgccgg aaatcattct tctcaacagc
     2461 catgacggct catcaagcta ccagatgctg ccggggctat ttcgtggcgt ttgtacgaac
     2521 ggtcttgtct gcggccagtc attcggagaa gtgcgggtac cgcataaagg caatgtcgtc
     2581 gagaaggtaa ttgaaggggc ttacgaagtg ctcggggcct ttgaccgggt ggatgagaaa
     2641 cgcgatgcaa tgcagtcgct ggcgctgcca gcaccggccc gtaacgccct ggcgaatgcc
     2701 gcgcttaagt atcgctttgg tgaagaccac cagccggtca cggtgtcgca gttgctgacc
     2761 ccacgtcgcc gggaggactg cagcgatgac ctgtggaccg tataccagcg cgtacaggag
     2821 aacctgatga aaggaggtct gtcagggcga accgcacagg ggaaaagcag ccgcacccgt
     2881 gctgttaccg gcattgacgg cgatgtgaag ctcaatcgcg ccctgtgggt aatggcagaa
     2941 aacatgctcg cgtttttcga gcgttaaaca accgttccgg cataaggaga tagtttaatg
     3001 gatacacaca accctgagtt cgctgagcag gcagcaatta ctgcctcggt ggtccctgac
     3061 gagctacgca tcggcttctg gccacagcac tttggctcca tcccacaatg gataaccctt
     3121 gagccccgaa tctttgcctg gatggaccgc ctgtgcactg actatcacgg cggtatctgg
     3181 aacttttcga cgctcagcaa cggaggcgct tttatgtatc ctgagtcgga ggatgatgaa
     3241 aagtgggcat tgtttaacag catgaacggc aatggagcag aactcaccag tgaagcggca
     3301 gggatagtcg cctgtctgat gacatacagt caccacgcct gtcgcaccga gtgtgatgca
     3361 acgactgagc actattatcg cttacgcgac ttcgcgctga accatccaga gtgcaactca
     3421 attatgcatc tcatcgactg aggagagtca agcatgagta ccgcgttatc cccattgtca
     3481 tcacagcctg aactctttcc gctggcggtt atggctcaac atggttgcct gcttcccgcg
     3541 acttccggat tgacacccta tgcccaacgg accattcgcc gggctatcaa tctgctggat
     3601 aaatatctgc gccagcccgg catatctttt acctcaagca ctgctgcccg tgactggctt
     3661 cggttacagc tggcaggaca agaacgggaa gtgtttatgg tgctctatct tgataatcag
     3721 catcgtttac tggagagcga aacgctgttt gccggttcag ttaatcatgt ccaggtccat
     3781 ccccgcgaag tagtgaagtc agcacttcgc ttaaatgctg cagctgccgt gctggcgcat
     3841 aaccatccat ccggtgatcc ggagcccagt caatgtgacc gcaacattac gggcaggctt
     3901 aaagaagcgt tagcactggt tgatgtgaac actctcgatc atctggtcat cggctcagag
     3961 ggcattgtgt cgttcgcgga gcgaggctgg atatgaaaat catcagtaaa cgccaggcaa
     4021 tgaccctata ccggcaacat cctggctccc ggctgtttcg cttcagtaca ggaaaatata
     4081 aatggtcagg cagtatctgc cattatgctg gccgcgaggt ggaagatata cgcggcgttc
     4141 tggccgtatt tgctgaacgc cgtcagggcc gcaatggtcc ctatgtcatt cttcgaagtg
     4201 tcactctcaa ttaatctttc cctttaatca ggaacaataa tgaacaatca ctccgaatcc
     4261 ggtaccaagc cggagaatcc cgcctgccag cagtggggac taaagcgcac aatcacaccc
     4321 tgttttggcg ctcgtctggt gcaggaaggc aaccgggtgc attttctcgc tgacagggcc
     4381 ggattcaacg gtactttcag cgacaacgat gcattgcgcc tcgaccaggc ctttccgttg
     4441 atactcaaac aactggagtt gatgctcacc agcggagaac tctctcatct gcatcagcac
     4501 tgcatcacgc tataccacaa tggactgact tgcgaagctg acacactcgg ctcctgtggc
     4561 tatgtctaca tcgctattta tcccgaccag cctgaacccc attaactcta agcttatctc
     4621 ttaaactcat agagcaaaca tcatgcaaac cttatcatca cacccgacac gggcgactca
     4681 gcccaatctg tcacccgttg aaacctggca acggctgctg actcatcttt tatcgcagca
     4741 ctatggcctg acgttgaatg acacaccgtt cagtaatgaa accaccatac gagaacatat
     4801 tgatgctgga gtatcgctca gcgatgcggt gaactttctt gtcgaaaaat acgggcttgt
     4861 ccgtatcgac cgaaagggat tttcgtggca agaacaaacc ccgtatattt ccatagtgga
     4921 tattctgcaa gcaaggcgct ctaccggctt gctaaaaact aatgtgaaat aaacgcttaa
     4981 atacagagca gactaaagga aagcaaaatg ctaatctcac agacgaagag acttctactg
     5041 taacctcccc tcccctgcaa aaaacctgac attttctttt aggaccaaca atgggaccaa
     5101 attgaaaatt gaattaaaga ttacaaacta ttaaacaaca agttacgcaa ccaattcaga
     5161 ctccgccagc caatcatgat tggacggtgt aaggactaca ccaacaaaaa caggaagtta
     5221 taagtctcag caggacaccg accagacggt gaggagacaa aaaaggatac gcaacggagt
     5281 cgcgactccc tatgacatta aagcccgctg atgcgggctt ttttattggt cctgacccgc
     5341 cctgaataga attcatccaa ttacagtcca gacatataca gtatccctca gctcagtatc
     5401 agcttctcga caaatggtca ctgttattta cccgttgcca gaaagagaat aatttgcccc
     5461 catagccagc cttcccggaa tctgtatatt cattaatatt gttatcgatt ccatcaagta
     5521 acataggttg gatgcgattg atcttactca cattagtcga cacggaactc agtaagttga
     5581 actctgaaaa agcagtttaa atagcattta aacaaatgcc accagttcga aaaagagta
//
LOCUS       DADSZD010000224         5610 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.226, whole genome shotgun sequence.
ACCESSION   DADSZD010000224 DADSZD010000000
VERSION     DADSZD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.226"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            172..2379
                     /gene="tssI"
                     /locus_tag="J1Z28_004113"
     CDS             172..2379
                     /gene="tssI"
                     /locus_tag="J1Z28_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA7718886.1"
                     /translation="MSFVSTGNKSVGGGGPAITTPPVVGESGGVSADSAATGVADVAE
                     QMAEQAADLFGALPEPSGLVKAAVAAAQAAAAAAGIADMAGAAQDAMSSLISGPGAHN
                     VTVSGSAVPPQMLLFASLNGSETLGSLFTYVVQLKTSDTLNLGYVSPAANLPLKPMVG
                     KDLCVNIELDGGGKRYISGLVTAARVVGHEGRSVTYELRIEPWLKLLTHTSDYKAFQN
                     KTVVEILDEVLAEYSFPVEKRLVENYPSRTWPVQYGETDFDFIQRLMQEWGIYWWFEH
                     SENSHTLVLADAISAHKACPDSPLVSYYQAGLKLDKEFIHTITANESLRTGQWVLDDF
                     DFMKPRSLLANTVANPRETGHADFEHYEWPGDYFTKDEGEMLTRIRMEEQRSPGSRVH
                     GGGNIRTLMTGYTFTLENYPTAEVNQEYLLVQTTLFIQDNAQHSGQEQHFSYSTSFEL
                     HPTCEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDENS
                     SCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPG
                     MASQSGIFSHSLYGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTIMVDRT
                     KTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQLTLRCGNAALVL
                     HKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTANDIAPLPSPDVSE"
     gene            2404..2846
                     /locus_tag="J1Z28_004114"
                     /pseudo
     CDS             2404..2846
                     /locus_tag="J1Z28_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            2867..>5610
                     /locus_tag="J1Z28_004115"
     CDS             2867..>5610
                     /locus_tag="J1Z28_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBA7718887.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVAAFAFFTCGFF
                     AGLVLGFLADQIASGVLQLGEAIGRSIHSTAGKILTGSPNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQPAARKDDHTECDAVIEDGSPNVFLGGGTQTVLAISPE
                     IPDWLRQVVDVLFVVASLLGGLAGAWRQAAKLGSKFGTKCAAKFIGGQLVGMGVSEAV
                     MGLFCNPVDVTTGQKILLPETDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEIT
                     LREDETYITFIGVQGRELSYPKAMLTPGHQIFDPEEQFYLSRLHDGRYVLHYTDRSYY
                     VFDEFDDHGVAPLRFMETPYRQRIAFGRENGRLVRVASSSGHHLLLHRTMTPAGERLS
                     HIELVKGGRPGNLVEYRYDDNGQLTGVVNRAGVTARQFAYENGLMTEHRNATGFTCTY
                     RWQEIDGFPRVVEHTTSDGEHYRFQYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRT
                     PGGGLYRFTYNENHFPVAVELPGERRVTLEYDTLSRVVKETDAAGRVTQTQWNGSFAE
                     ITRRALDDDHVWKADYNEHGQVIRETDPEGRITRYGYDEQGLAVSRTDARGGEAALVH
                     DARGQLRRYTDCSGCATDYEYDEGGNLTAVTDAEGKTVRIRYNRLGLPETVNHPGKQQ
                     DRYTWNALGLLSSHRRITGSVQSWQYTPRGLLALHVDEEKRETRWHYTAEGWIASLSN
                     GNGAQYRFSHDADGRLTGEQRPDGLIRMFVLNAGGFPVIIQTQGTEGGVRNERQERDA
                     LGRLLRSDTQHSTRTFSYNRLDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTA
                     EHSVHGSIKYQRDALGNPTDITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLH
                     R"
BASE COUNT         1330 a         1468 c         1689 g         1123 t
ORIGIN      
        1 atacattaca taagcgcatt atcaataaga atgaaattgt atgggagagt cgttgttcga
       61 taaatcagcc agcatcattt tatctttctg ttaactatac agaaaatagt gttcagcttg
      121 aaaaagaaat ttgttattag cagatgacta catttttcaa aaggatccat catgagtttt
      181 gtatccacag gtaataaatc agttgggggc ggcgggcctg ccatcactac gccgccagtc
      241 gtcggagaaa gtggtggtgt gagtgcagat tctgccgcaa ccggtgttgc tgatgtcgcc
      301 gaacaaatgg cggaacaggc ggcggatctg tttggtgcgc tgccagaacc gtcaggactg
      361 gtaaaagcag cagtggcagc agcgcaggcc gccgccgccg cggcagggat agcggatatg
      421 gcaggggccg cacaggatgc aatgtcatcc cttatctccg ggccgggggc gcataacgtc
      481 acggtaagcg gcagtgctgt gccgccgcag atgttgttgt ttgccagcct taacggctcg
      541 gaaacactgg gtagcctgtt tacttacgtg gtacagctta aaacctcgga caccctgaac
      601 ctgggttatg tatctcccgc cgccaacctg ccgctcaaac cgatggtggg caaagacctg
      661 tgcgtcaaca ttgaactgga tggcggcgga aaacggtata tcagtggcct ggtaacggcg
      721 gcgcgggtag tgggtcatga gggccgttcg gtaacgtatg agttacgtat tgagccgtgg
      781 ctgaaactgc taacccacac cagcgactac aaggcgttcc agaataaaac ggtggtggaa
      841 attctggacg aagtgctggc tgaatattct tttccggtgg aaaaacgtct ggtggaaaac
      901 tacccgtcac gcacctggcc ggtgcagtac ggtgaaaccg actttgattt catccagcgc
      961 ctgatgcagg agtggggcat ctactggtgg tttgagcaca gcgagaacag tcatacgctg
     1021 gtgctggcgg atgccatcag cgcccataag gcgtgcccgg actcaccgct ggtcagttac
     1081 taccaggcag ggctgaagct ggataaggag tttatccata ccatcacggc caatgaaagt
     1141 ctgcgtaccg ggcagtgggt gctggacgat ttcgatttta tgaagccacg ctcattgctg
     1201 gcgaacaccg tggcaaaccc gcgtgaaacc ggtcatgcag actttgaaca ttacgaatgg
     1261 ccgggagact acttcacgaa ggatgaaggc gagatgctga cgcgcatccg catggaagaa
     1321 caacgaagcc ctggtagccg ggtgcacggc ggtggcaaca tccgcacact gatgacgggc
     1381 tataccttca cactggagaa ctacccgacc gccgaagtga atcaggaata cctgctggta
     1441 cagaccacgc tatttataca ggacaatgcg caacacagcg gccaggagca acatttctct
     1501 tactcaacca gctttgaact gcatcccacc tgcgaagtct accgcccgca gcgaacgctg
     1561 agcaaacccc acactaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa
     1621 atctggacgg ataaatatgg ccgggtgaag gtgcagtttg gctgggatcg ctacggaaag
     1681 aatgacgaaa acagctcctg ctggatccga gtgagctacc cgtgggcagg gaaaggattc
     1741 ggcatgatcc agatcccgcg tattggtcag gaagtgctgg tggatttcaa aaacggcgac
     1801 ccggatttac ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccctggggg
     1861 ctgccgggca tggcgtcgca gagtgggata ttcagccact cattgtatgg cgggccgact
     1921 aacggaaaca tgctgcgctt cgatgacaaa acgggtgcgg aagaggtgaa gttccatgcg
     1981 gaaaaagacc tgaataccac ggtaaaaaac aatgaaaccc acacgatcat ggtcgatcgc
     2041 acaaaaacca ttgtcaaaaa tgaaaccatc agcgtgggag aagatcgcaa tgccaccatc
     2101 acaaaaaatg atggtctttc cgtcaaacta gctcaaacca tcaatgtcgg gacgacctat
     2161 cgtctggatg ttggcgatca attaactctt cgctgcggca atgcagcgct tgtattacac
     2221 aaggatggct cgattgagtt ttgtggaaaa caacttttac ttcacaccag cgatgttatg
     2281 caactcattg gcagtggcat tgatatgaat ccagatggcg gcacagccat gactgcgaat
     2341 gatattgccc cgttaccttc cccggacgtt tctgaataaa aaaatataaa aggaattttt
     2401 tctatgacca gcctttacca tattcaggag ggctgcttta ccctgcctga aacttttctt
     2461 gatcgtaccg tcaatatttt cgtcccggcg agtaatgagc gggcctcccc cagtctgaat
     2521 atttcccgtg acactctcaa acccgatgaa aacctgacca cctatattga ccgccagata
     2581 gtcttgatga aaaaaacctg ggccagcaca gggtactttc gcggggaacg gcactggcgg
     2641 gaactggcga agctgccatg acaggtgaac agattctcgc aactcatcgt tctggaaaaa
     2701 cagaagtgta ccagcgtcag gccgggttta tctccgggcc aggaaaggta ctggtgttga
     2761 cgctgaccag tcagtgtcct tttgatgagc actctgacca gctatggact gcctggttga
     2821 ccagttttca gcctgctaaa agttaaaaca acatggaggt gttgtcatgt atgaagccgc
     2881 acgggtggat gaccctatct accacaccag cgctctcgcc gggtttttga ttggggctat
     2941 cattggcatt gccattattg ccgttgccgc ctttgccttt tttacctgcg gcttttttgc
     3001 cggtctggtg ctgggttttc ttgctgacca gatagcgtcc ggagtccttc aactggggga
     3061 agccatcggg cgctcgatac actccaccgc gggaaaaatt ctgaccggct ctcccaatgt
     3121 cagcaccaac agccgacctg ctgcccgcgc cgtactgagt acggtgatat gcgaagacca
     3181 ttctccggaa atccgtatcg cccagggttc cggcaatatc tacatcaaca gccagcccgc
     3241 cgcccgtaag gatgaccaca ctgaatgcga cgcggtgatt gaggacggct ctcccaacgt
     3301 ctttctcggc ggcggtacac agacggtgct ggcaatcagc ccggaaatcc cggactggct
     3361 gcgtcaggtg gtggatgtgc tgtttgtggt tgccagcctg ctgggcgggc tggccggagc
     3421 ctggcgtcag gcggcaaagc tgggttcgaa attcggcacc aaatgtgcgg caaaatttat
     3481 tggcgggcaa ctggtcggga tgggcgtatc cgaggctgtc atgggactgt tctgcaaccc
     3541 ggtggatgtg accaccgggc aaaaaatcct gctgccggaa acggatttta ccctgccggg
     3601 ccgtctgccc gtcacctgct cgcgctttta tgccagccac ctggaaacag agggactgtt
     3661 gggcagagga tggcggctga actgggagat aaccctgcgt gaggatgaga catatatcac
     3721 cttcatcggt gtacagggcc gggaactgag ttatccgaaa gcgatgctga cgccgggtca
     3781 ccagatattc gacccggaag agcagtttta cctgagtcgc ctgcatgacg ggcgctatgt
     3841 gctgcattac accgaccgta gctattacgt gtttgatgaa tttgatgacc acggcgtcgc
     3901 gccgttacgt tttatggaaa ccccgtaccg gcagcgtatt gccttcgggc gtgagaacgg
     3961 caggctggtg cgggtggcct ccagcagcgg acaccatctg ttgttgcacc gcacgatgac
     4021 gccggcggga gaacgcctgt cgcatattga acttgtgaag ggcgggcgtc ccggtaacct
     4081 ggtggaatat cgttacgatg ataacgggca actgaccggc gtggtgaacc gggccggtgt
     4141 gacggcgcgc cagtttgcgt atgaaaatgg cctgatgacg gagcaccgca acgcaacagg
     4201 cttcacctgt acctaccgct ggcaggagat tgatggtttc ccccgcgtgg tggaacacac
     4261 gaccagcgac ggcgagcatt accgctttca gtacgatttt gcgggcgggc agacggtggt
     4321 aaccggcagg ccggagcaga agtggcagtg gtggtttgat gaagagacgt atgtgaccgc
     4381 gcaccggacg ccgggcggcg gactgtaccg cttcacgtac aacgaaaacc atttcccggt
     4441 cgccgtggaa ctccccggcg aacgcagggt aacgctggaa tacgacactc tgagccgggt
     4501 ggtgaaagag acggacgcgg cagggcgggt gacacagacg cagtggaacg gcagctttgc
     4561 tgaaatcaca cgccgggcgc tggatgacga ccacgtatgg aaggcggatt acaacgaaca
     4621 cggccaggta atacgggaga cggacccgga agggcgcatc acgcgctacg ggtatgatga
     4681 acaggggctg gcggtcagcc gcacggatgc gcggggcggc gaggctgcgc tggtgcacga
     4741 tgcgcgcggc cagctgaggc gttacaccga ctgttcaggg tgcgccacgg actatgaata
     4801 cgatgagggc ggcaacctga cggcggtgac ggacgcggaa gggaaaacgg tacgtatccg
     4861 ctataaccgt ctggggctgc cggagacggt aaatcatccc ggtaaacagc aggaccgtta
     4921 cacctggaac gcgctggggt tactgagcag ccaccgtcgc atcacgggta gtgtgcagtc
     4981 ctggcagtat acgccgcgcg ggctgctggc gctccacgta gatgaagaga aacgggagac
     5041 gcgctggcat tataccgcgg agggatggat tgccagtctg agcaacggca acggggcgca
     5101 gtaccggttc agccacgatg cggacggacg gctgacggga gaacagcgcc cggacggtct
     5161 gatacggatg tttgtgctga atgcgggcgg gttcccggtc atcatccaga cacagggtac
     5221 agaaggcggg gtgcgtaatg agcgacagga gcgtgacgcg ctgggtcgcc tgttgcgcag
     5281 tgacacgcag cacagtacac ggaccttcag ctacaaccgt cttgaccaga taacggaagt
     5341 gacgctcact cccacggaag aaggggaacg gctgcaccac atgcaggcgg acacggtgcg
     5401 ctttgcgtat gaccgcagcg ggtggctgac ggcagagcac agcgtgcacg gcagtataaa
     5461 atatcagcgc gacgcgctgg gcaatcccac cgacattacc ctgccagacg ggcagcatct
     5521 cagtcacctg tactacggca gcgggcatct gctccagaca gcgctggacg gtatcacggt
     5581 cagtgaatat gagcgtgaca gtctgcaccg
//
LOCUS       DADSZD010000225         5604 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.227, whole genome shotgun sequence.
ACCESSION   DADSZD010000225 DADSZD010000000
VERSION     DADSZD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.227"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            210..4460
                     /gene="fdeC"
                     /locus_tag="J1Z28_004116"
     CDS             210..4460
                     /gene="fdeC"
                     /locus_tag="J1Z28_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HBA7718888.1"
                     /translation="MSRYKTDNKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMENTTVTADNNVEKNVASLAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKNFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSENDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDVEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAITAEVNYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIGGKGGQTVSLGLVVSKATHGLK
                     NVQWEAPSLLAAGGKITGQGNQWQVTLPAYQAGKDNYYAISAIAYDNKGNASKRVQTE
                     VVISGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDDMS
                     KTVTAELRATMMDVSNSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     LVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAAGSTASGW
                     TDNGDGTWTAQISLGTTAGELDVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKTPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGELEIEASVKNSQKTVKVKF
                     KADFSTGQASLEVDAAAQKVANGKDAFTLTATVKDQYGNLLPGAVVVFNLPRGVKPLA
                     DGNIMVNADKEGKAELKVVSVTAGTYEITASAGNDQPSNAQSVTFVADKTTATISSIE
                     VIGNRAVADGKTKQTYKVTVTDANNNLLKDSEVTLTASPENLVLTPNGTATTNEQGQA
                     IFTATTTVAATYTLTAKVEQADGQESTKTAESKFVADDKNAVLAASPERVDSLVADGK
                     TTATLTVTLMSGVNPVGGTMWVDIEAPEGVTEADYQFLPSKNDHFASGKITRTFSTNK
                     PGTYTFTFNSLTYGGYEMKPVTVTINAVPADTEGAEEK"
     gene            complement(4585..5442)
                     /locus_tag="J1Z28_004117"
     CDS             complement(4585..5442)
                     /locus_tag="J1Z28_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7718889.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLSAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKLPDEEVKL
                     RRHHLVRCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEYFVEIDSNNIVC
                     EIFIPVRPV"
BASE COUNT         1507 a         1291 c         1497 g         1309 t
ORIGIN      
        1 cccataaata aaaatataat cccgcgccca accacctgat gagtggctac aaacactgga
       61 tatattaggt ggcggtgtac tttcttacat aaaggtattt ccttttctgt ggaaaaggaa
      121 accgggaaat ccccggtttt tctgacaagc agacgccatt atttgtgtct gcctatgttc
      181 gttaattcgt tcatcaggaa attatctcaa tgtcacgtta taaaacagac aataaacagc
      241 cacgatttcg ttattcagtt ctggcccgct gcgtggcgtg ggcaaatatc tctgttcagg
      301 ttctttttcc actcgctgtc acctttacgc cagtaatggc agcacgtgcg cagcatgcgg
      361 ttcagccacg gttgagcatg gaaaatacta cggtaactgc tgataataac gtggagaaaa
      421 atgtcgcgtc gcttgccgct aatgccggga catttttaag cagtcagcca gatagcgatg
      481 cgacacgtaa ctttattacc ggaatggcca ccgctaaagc taaccaggaa attcaggagt
      541 ggctcgggaa atacggtact gcgcgcgtca aactgaatgt cgataaaaat ttctcgctga
      601 aggactcttc gctggaaatg ctttatccga tttatgatac accgacaaat atgttgttca
      661 ctcagggggc aatacatcgt accgacgatc gtactcagtc aaatattggt tttggctggc
      721 gtcatttttc agaaaatgac tggatggcgg gggtgaatac ttttatcgat catgatttat
      781 cccgtagtca tacccgcatt ggtgttggtg cggaatactg gcgtgattat ttgaaactga
      841 gcgccaatgg ttatatccgg gcttctggct ggaaaaaatc gccggatgtt gaggattatc
      901 aggaacgccc ggcgaatggc tgggatattc gtgctgaggg ctatttacct gcctggccgc
      961 agcttggcgc aagcctgatg tatgaacagt attatggcga tgaagtcggg ctgtttggta
     1021 aagataaacg ccagaaagat ccacatgcga ttaccgctga agtgaattac acgccagtgc
     1081 ctcttctgac cctgagtgcc gggcataagc agggcaagag tggtgagaat gacactcgct
     1141 ttggcctgga agttaattat cggattggcg aacctctgga aaaacaactc gatacagaca
     1201 gcattcgcga gcgtcgaatg ctggcaggca gccgctatga cctggttgag cgtaataaca
     1261 atatcgttct tgagtatcgc aaatctgaag tgatccgtat tgctctgcct gagcgtattg
     1321 gaggcaaggg cggccagacg gtttccctgg ggcttgtggt cagcaaagca actcacggtc
     1381 tgaaaaatgt gcaatgggaa gcgccgtctt tgctggccgc aggcggaaaa attacggggc
     1441 agggcaatca gtggcaagtg acgctcccgg cttatcaggc aggcaaagac aattattatg
     1501 cgatttcagc gattgcctac gataacaaag gcaatgcctc gaaacgtgtg cagacagaag
     1561 tagttattag cggagctggt atgagcgccg atcgtacggc gttaacgctt gacggtcaga
     1621 gccgtattca aatgcttgct aacggtaatg agcaaaagcc gctggtgctg tctctgcgcg
     1681 acgccgaggg ccagccagtc acgggcatga aagatcagat caagactgaa ctaaccttca
     1741 aaccggctgg aaatattgtg actcgtaccc tgaaggccac taaatcacag gcaaagccaa
     1801 cactgggtga gttcaccgaa actgaagcag gggtgtatca gtctgtcttt actaccggaa
     1861 cgcagtcagg tgaggcaacg attactgtta gcgttgatga catgagcaaa actgtcactg
     1921 cagaactgcg ggccacgatg atggatgtgt caaactccac cctgagtgct aacgagccgt
     1981 caggtgatgt ggttgctgat ggtcagcaag cctacacgct gacactgaca gcggtggact
     2041 ccgagggtaa tccggtgacg ggagaagcca gccgcctgcg acttgttccg caagacacta
     2101 atggtgtaac cgttggtgcc atttcggaaa taaaaccagg ggtttacagc gccacggttt
     2161 cttcgacccg tgccggaaac gttgttgtgc gtgccttcag cgagcagtat cagctgggca
     2221 cattacaaca aacgctgaag tttgttgccg ggccgcttga tgcagcacat tcgtccatca
     2281 cactgaatcc tgataaaccg gtggttggcg gtacagttac ggcaatctgg acggcaaaag
     2341 atgctaatga caaccctgta actggcctca atccggatgc accgtcatta tcgggcgcag
     2401 ctgctgctgg ttctacggca tcaggctgga cggataatgg cgacgggacc tggactgcgc
     2461 agatttctct cggcactacg gcgggtgaat tagacgttat gccgaagctc aatgggcagg
     2521 acgcggcagc aaatgcggca aaagtaaccg tggtggctga tgcattatct tcaaaccagt
     2581 cgaaagtctc tgtcgcagaa gatcacgtaa aagccggtga aagcacaacc gtaacgctgg
     2641 tggcgaaaga tgcgcatggc aacgctatca gtggtctttc gttgtcggca agtttgacgg
     2701 ggaccgcctc tgaaggggcg accgtttcca gttggaccga aaaaggtgac ggttcctatg
     2761 ttgctacgtt aactacaggc ggaaagacgg gcgagcttcg tgtcatgccg ctcttcaacg
     2821 gccagcctgc agccaccgaa gccgcgcagc tgactgttat tgccggagag atgtcatcag
     2881 cgaactctac gcttgttgcg gacaataaaa ctccaacggt taaaacgacg acggaactca
     2941 ccttcaccgt gaaggatgcg tacgggaatc cggtcaccgg gctgaagcca gatgcaccag
     3001 tgtttagtgg tgccgccagc acggggagtg agcgtccttc agcaggaaac tggacagaga
     3061 aaggtaatgg ggtctacgtg tcgaccttaa cgctgggatc tgccgcgggt cagttgtctg
     3121 tgatgccgcg agtgaacggc caaaatgccg ttgctcagcc actggtgctg aatgttgcag
     3181 gtgacgcatc taaggctgag attcgagata tgacagtgaa ggttaataac caactggcta
     3241 atggacagtc tgctaaccag atcaccctga ccgtcgtgga cagctatggt aacccgttgc
     3301 aggggcaaga agttacgctg actttaccgc agggtgtgac cagcaagacg gggaatacag
     3361 taacaaccaa tgcggcaggg aaagtggaca ttgagcttat gtcaacggtt gcgggggaac
     3421 ttgagatcga ggcctcggtg aaaaactctc agaagacggt caaggtgaaa ttcaaggcgg
     3481 atttcagtac cggtcaggcg agcctggagg tagacgccgc tgctcaaaaa gtggcaaacg
     3541 gcaaagatgc ctttacgctg acggcaacgg ttaaggatca atacggcaac cttcttcctg
     3601 gcgctgtggt cgtctttaat ctgcctcggg gcgtcaaacc gcttgcagac ggtaatatca
     3661 tggtgaacgc cgacaaggag ggtaaagcgg aactgaaagt ggtttccgtg actgccggaa
     3721 cctatgagat cacggcgtca gcaggaaatg accagccttc gaatgcgcag tctgtaacgt
     3781 ttgtggctga taagactacg gcgaccatct ccagtattga ggtgattggc aaccgtgcag
     3841 tggcggacgg caaaaccaaa cagacgtata aagttacggt gactgatgcc aataacaacc
     3901 tgctgaaaga tagcgaagtg acgctgactg ccagcccgga aaatttagtt ctgactccca
     3961 atgggacggc gacaacgaat gagcaagggc aggctatttt caccgccacg accactgtcg
     4021 cagcgacata tacactcacg gcgaaagtgg aacaggccga cggtcaggaa tcgacgaaaa
     4081 ctgccgaatc taaattcgtc gcggatgata aaaacgcggt gctcgctgca tctccagagc
     4141 gtgtagattc tctggtggcg gacgggaaga ctactgcaac actgacggtt actctgatgt
     4201 cgggtgtcaa ccccgtagga ggaaccatgt gggtcgacat tgaggctccg gaaggggtga
     4261 cagaggcgga ttatcagttc ctgccgtcga aaaatgacca tttcgcgagc gggaaaatca
     4321 cgcgtacatt tagtaccaac aagccaggta catacacatt cacattcaac tctttgacat
     4381 atggagggta tgaaatgaaa ccagtgactg tgacaattaa cgccgttcct gcagatactg
     4441 aaggcgctga ggagaaataa aaacgacgat gtaataaata ataatggcgt tgacatgcgc
     4501 agcgccatta tttactgaat acctaccctg gtaccgatta gacgctggca gggtagggta
     4561 aatcttatgt gatggagtta caggctaaac cgggcgaacg gggataaaaa tttcgcaaac
     4621 aatgttattg ctatcaattt cgacgaagta ttcattgtaa taaaatcgct caatatcact
     4681 gccgttcact ctggctaacc ccctggcagg taattccatc agatagaggt ttcgcgccca
     4741 ttcagggtag tgctcccact caccttcata gcggaaagag gcatacagtc ctcggttaat
     4801 tgtccactct ttcggcatct ctgaatcggt ttgtttgcat accgtaccgg caaaagtttc
     4861 aactgtgagc aaatcgaccg attttgttga tggttcgaac gtggagacga aatagatatc
     4921 cgtcttaaaa ttcttcatac atctgactaa atgatgacgt cgcagtttga cttcttcatc
     4981 cggtaattta ttcgacactt caatatcata tttaaatgaa ttgccaaaaa taggtgtctc
     5041 aggaaagttg actaatctac actccgtctt ttgctgttga cgtattaaaa aagaagggaa
     5101 gatatttgcg agatcccaat aatcacggtg gcggtattca cggggagagc agccgaataa
     5161 ctttttaaat tcgcggctga atgactgctg tgaatcaaaa tgcaaagaga gtgcaatatc
     5221 aagcatagat ttcgcggaga gccggacaag aatggcggca cgacaaagcc ttcgtttgcg
     5281 aatgtattct cccaaaggca catgcatgaa atttcggaac agaagctgga tgttgcgtct
     5341 gctgtagcca gatttctgtg cgatatcttc gattgaaata gggtgctcaa gattgcactc
     5401 aatccactcc aggagctgct gtagaatctt ctgcctgatc atcacatact ccgttaattc
     5461 tttgtcatat cgcacatttt cgttatgtcc gggccctaag tgtatcaaaa acagcaagtc
     5521 ccaaggcaaa agcactgata gcgtctgctt cattgcccca aaaatagaga ggtttcagtc
     5581 ctacattatt aatgaatttt ttgc
//
LOCUS       DADSZD010000226         5518 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.228, whole genome shotgun sequence.
ACCESSION   DADSZD010000226 DADSZD010000000
VERSION     DADSZD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.228"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(53..2122)
                     /gene="glyS"
                     /locus_tag="J1Z28_004118"
     CDS             complement(53..2122)
                     /gene="glyS"
                     /locus_tag="J1Z28_004118"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBA7718890.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEVLLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2132..3043)
                     /gene="glyQ"
                     /locus_tag="J1Z28_004119"
     CDS             complement(2132..3043)
                     /gene="glyQ"
                     /locus_tag="J1Z28_004119"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBA7718891.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALESPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3138..3437)
                     /locus_tag="J1Z28_004120"
     CDS             complement(3138..3437)
                     /locus_tag="J1Z28_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718892.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPLPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3612..4607
                     /locus_tag="J1Z28_004121"
     CDS             3612..4607
                     /locus_tag="J1Z28_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBA7718893.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLLFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCVTLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4649..5086)
                     /locus_tag="J1Z28_004122"
     CDS             complement(4649..5086)
                     /locus_tag="J1Z28_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBA7718894.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5132..5473)
                     /locus_tag="J1Z28_004123"
     CDS             complement(5132..5473)
                     /locus_tag="J1Z28_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718895.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
BASE COUNT         1347 a         1434 c         1343 g         1394 t
ORIGIN      
        1 tttagcgata aaaaaagcct gccagatggc aggctattta ataacggcgt tattactgca
       61 acagcgaaat atccgcaact cgcaggaaca gttcgcgcag tttctccagc atggtaagac
      121 ggttgattcg caattctttg tcatcaacca tgaccatcac tttatcgaag aaggcatcga
      181 ccggttcacg cagctcagcc agttcgacca gcgcatcctg gtaacgacct tccgcaaagt
      241 acggctccag cttgtcacgt agcaccacaa cctgcatcgc cagtttaatt tcttccggct
      301 ctttcagagt agaggcattc acgcgatcgc tcagcacttc atcagatttc gccaggatgt
      361 tagatacacg cttgttcgcc gccgccagtg ctgcagctgc ttccagggta cggaagtgcg
      421 ataccgcttt catacgggca tcgaaatcag ccggacgagt cggacgacgc gccagtaccg
      481 cctggatggt gtcaacggtg taaccttcgt cctgatacca ggcgcggaag cgaccgagca
      541 taaagtcgat aacatcatca accacgttgg cgttagtcag cttatcgcca tacagacgca
      601 ccgcttcttc ggtcagcgtt tgcagatcaa ggttgaggtt cttctcaacg ataatacgca
      661 gcacgccaag tgcggcacga cgcagcgcaa acgggtcttt gtcgcctttc ggatgctggc
      721 cgataccgaa gatacccgcc agggtatcca tcttgtcagc aatcgccagc gcacaggcta
      781 ccgggttgga cggcaggtca tcaccggcaa agcgcggctg atactgctca ttcagcgcaa
      841 cagctacgtc ttccgcttcg ccatcgtgac gcgcgtagtg catccccata acgccctggg
      901 tgtcggtgaa ctcgaagacc atgttggtca tcaggtcaca cttagacagc aggcccgcac
      961 gggtagcgtg gttaacgtca gcgccaatct gctcagcaat ccagccagcc agcgcctgaa
     1021 tgcggtcagt tttgtcgcgc agcgtcccca actgttgctg gaacaacacg gtttgcaggc
     1081 gcggcaggtt atcttcaaga cgttttttac ggtcggtatt gaagaagaac tcggcatccg
     1141 ccagacgtgg acgaacgact ttctcgttac cggagataat ctgctgcgga tctttcgatt
     1201 cgatgttggc aacgaagata aagttcggca gcagtttgcc gtcgttcgca tacaccggga
     1261 agtacttctg gtcacctttc atggtgtaaa ccagtgcttc agacggcacc gcgaggaatt
     1321 tctcttcgaa tttcgcggtc agaacgaccg gccactccac cagcgaagcc acttcttcca
     1381 gcaggctttc gcttaaatca gcgttaccgc caatcttacg tgctgcttct tcagcatcgg
     1441 ctttaatctt cgccttacgt tcttcgtaat cggcgatgac tttcccgcgc tcacgcagaa
     1501 tttccggata ctgatcggcg ttatcgatgg tgaattccgg ctcgcccata aagcggtggc
     1561 cgcgaatcac gcgatcggac tgaatgccca gaatggttgc cggaatgact ttgtcgccca
     1621 gcagcagggt cacggtgtgc accggacgca cgaagtgcac gtcgcttgcg ccccaacgca
     1681 tcagtttcgg gatcggcaat ttcgccagcg aagtcgcaac catattcggc agcagtactt
     1741 cggtgctttc gcccttcacg tgggcgcgat acagcagcca ttcaccttta tcggtagtca
     1801 gacgctcagc ctggtcaacg gtaataccgc aaccacgcgc ccaaccttct gccgctttgc
     1861 tcggtttgcc ttcagcgtcg aacgcctggg caattgccgg gccgcgtttt tcgatttcac
     1921 gatccggttg cgcttccgcc aggttagcta ctttcagcgc cagacgacgc ggagcagcaa
     1981 accattgaac ggtgccgtgt gcgaggccag cgttatccag ctccgcagta aagttcgcag
     2041 caaaggactc agccaggctg cgcagtgctt ttggtggcag ctcttcagtg ccgatttcca
     2101 ccagaaaagt tttctcagac atagccgcct cttacttatc tttgttgcac atcgggaagc
     2161 cgagggcttc acgggaagcg tagtatgctt cagccactgc tttggtcagg gtgcgaattc
     2221 gcagaatata gcgctgacgc tcggtgacgg agatggcttt acgcgcatcc agcaggttga
     2281 agctgtgggc ggctttcaga atacgctcgt aggctggcag cggcagcgga ctttccagcg
     2341 ccagcaactg ctgcgcttct ttctcgtact gctcgaagca ggtgaacagg aagtccacat
     2401 ccgcgtattc gaagttgtaa gtggactgct ccacttcgtt ctgatggaac acgtcgccgt
     2461 aggtggtttt acccagcggg ccgtcgctcc agaccaggtc gtaaacgctg tctacgccct
     2521 gaatgtacat ggccagacgt tccagaccgt aggtgatctc accggtaacc ggtttacatt
     2581 ccagaccacc aacctgctgg aagtaagtga actgcgtcac ttccatgccg tttagccaca
     2641 cttcccagcc cagcccccag gcacccagcg tcgggttttc ccagttatct tccacgaaac
     2701 ggatgtcgtg gatggtcggg tccatgccca gctctttcag agaaccgagg tacagctcct
     2761 gaatattgtc cggcgatggc ttaatgacca cctggaactg atagtagtgc tgtaaacggt
     2821 tggggttttc gccgtagcga ccatcggtcg ggcgacgaga aggctgaaca taagcagccg
     2881 ccatcggttc tggccccagc gcgcgcagac aggtcattgg gtgagaggtt cccgcgccga
     2941 cttccatgtc caatggttga acaatggtgc agccctggcg agcccagtaa tcctgtaagg
     3001 tcaggatcaa gccctggaag gtcctggtat caaacttttg catattattt cgtgctggat
     3061 acgtgtggat ttaaaggaag ggatcagtat acccgctgga tggaagatat acagtacgaa
     3121 acgggaaaaa gcagggctta acgcatggaa agatgcaaaa aatgtccatc cgcgtcaaaa
     3181 gaacagacaa actgctcttt ccggaaggta taaccgcgca tttcatagct tccctggaac
     3241 tgttcgaagg cggtgacgtc gattttttgc tcgccggtgt tataacgttt tgccgcctga
     3301 tccttgcata acgcttccat attcaacgaa cgcagaggat caacctttac ccgttgtgct
     3361 ttctgcacgg gtagcggtgt agaacaaccc actagcacaa taagcgccat tgctggaaag
     3421 aaagcgttca tcatcattaa attaccgtct ggtttgcagg tgagtcttat tatttatatc
     3481 ggtatagtgg ctgatatctt acgaatttcc ttaagcctct ataagctgta gtgataaaac
     3541 tcagattatt ccagtgcgcc aactgaatga tttatcggtc agactttttt taaccagata
     3601 cagagacaca gatgcagccc aaaatttact ggattgataa cctgcgaggg atagcgtgtt
     3661 taatggtggt gatgattcac accactacct ggtatgtgac caatgctcat agtgttagcc
     3721 ccatcacctg ggatatcgcc aatgttctga attctgcctc tcgtgtcagc gtgccgctat
     3781 ttttcatgat ttccggctat ctcttttttg gcgaacgcag cgcccagccg cgccatttct
     3841 tgcgtatcgg cttatgtctg cttttttata gcgcaatcgc actgctctac attgcgctgt
     3901 ttacctccat caatatggag ttagcgctga aaaacctgct gcaaaagcca gtgttttacc
     3961 acttgtggtt tttcttcgca attgcggtga tttatctggt ttcaccgctg attcaggtga
     4021 agaacgtcgg cggaaaaatg ttgctggtgc taatggtggt gattggcatt atcgctaacc
     4081 caaacaccgt gccgcagaaa attgacggtt ttgaatggct gccaattaac ttatatatca
     4141 atggcgatac tttttactac atcctttatg gcatgttggg ccgcgctata gggatgatgg
     4201 acacacagca taaagcactg tcgtgggtga gcgccgcact gtttgcgacg ggggttttta
     4261 ttatctctcg cgggacatta tatgaattac agtggcgcgg aaattttgcc gatacctggt
     4321 atctttactg tgggccgatg gtttttatct gcgcaatcgc gttattgact ctggttaaaa
     4381 acacgctgta tacgcgtacc atttgcggac ttggcttaat ctcccgccat tcgttaggta
     4441 tatacgggtt ccatgcattg attatccatg cactgcgcac ccgggggatt gagcttaaaa
     4501 actggccaat actcgatatt atttggatct tttgcgtgac gttggcagcg agtttgttac
     4561 tttctatgct ggtacaacga atcgacagaa acagactagt gagttaaata aaagcccggt
     4621 tacattggac taaccgggct tatgtgaatt attcgctgta ttcttcctgg gtaacctgtg
     4681 tttcttttgg tgggtttatt ccggcatcac gaatattctt ctgcaccgct actgcaccaa
     4741 acaagcttaa gaagaaagcc agcccataaa agcctttttc gctgagtaat aacgttgcat
     4801 tccatagacc taccagcaac aacgcaacag agataataaa gaccgtcagg caggtcatat
     4861 aataaatggg ggtggtaggt atgccttcat acttgtcccg aacggtcttt tgataagacg
     4921 ccgcggaaaa caaacctaat accagtacgg caaaataata ccctttttca tttaactgca
     4981 tctctgcatt ccatagccct aacagatagg taacaatacc gccaataagg gctacccatg
     5041 aaacaatact aaatgccgga gaataggttg atattttgtt gtccatcaca gtattccttt
     5101 cattcctgaa tgtgtaagaa ctttatgttg ctcatgccga gcgaaacaaa cgcatttgac
     5161 caaacaagca gacaaaaaag gcgatgggat agatcaccat ttcataagca ttgataggtg
     5221 cgtttaaaac accgataaac aagagaccga tagtgattaa cgctaccaac tgacagacgt
     5281 ggataaaaaa atcatccagt tgcccaagtt tttctgcgcg aagatatgca tagttgccaa
     5341 aagttgccgt cattaacacg ccaagaaaat agccttttcc acttaacaat gggcatgaga
     5401 tccatagccc aaccagataa accagcgccc caacaacaaa taatagtttt gccacagtct
     5461 ttgaggtttt cataacgatc tccatatcta ccagcgatac attacgaata accaacga
//
LOCUS       DADSZD010000227         5513 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.229, whole genome shotgun sequence.
ACCESSION   DADSZD010000227 DADSZD010000000
VERSION     DADSZD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.229"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(44..340)
                     /locus_tag="J1Z28_004124"
     CDS             complement(44..340)
                     /locus_tag="J1Z28_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBA7718896.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            564..1280
                     /gene="tonB"
                     /locus_tag="J1Z28_004125"
     CDS             564..1280
                     /gene="tonB"
                     /locus_tag="J1Z28_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBA7718897.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            complement(1320..1718)
                     /gene="yciA"
                     /locus_tag="J1Z28_004126"
     CDS             complement(1320..1718)
                     /gene="yciA"
                     /locus_tag="J1Z28_004126"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBA7718898.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(1824..2363)
                     /locus_tag="J1Z28_004127"
     CDS             complement(1824..2363)
                     /locus_tag="J1Z28_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBA7718899.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(2393..3136)
                     /locus_tag="J1Z28_004128"
     CDS             complement(2393..3136)
                     /locus_tag="J1Z28_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBA7718900.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            complement(3164..3253)
                     /locus_tag="J1Z28_004129"
                     /pseudo
     CDS             complement(3164..3253)
                     /locus_tag="J1Z28_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3492..4130
                     /gene="ompW"
                     /locus_tag="J1Z28_004130"
     CDS             3492..4130
                     /gene="ompW"
                     /locus_tag="J1Z28_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBA7718901.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(4189..4626)
                     /locus_tag="J1Z28_004131"
     CDS             complement(4189..4626)
                     /locus_tag="J1Z28_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBA7718902.1"
                     /translation="MLESMASRIDNYPELRARIEQHLSETKNQIVQLETILDRNDISR
                     SVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYVFEQFEIACYTSLLAAAKNAGDTA
                     SIPIIEAILNDEKHMADWLIQHIPQTTEKFLIRSETDGVEAKK"
     gene            complement(4741..5241)
                     /locus_tag="J1Z28_004132"
     CDS             complement(4741..5241)
                     /locus_tag="J1Z28_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBA7718903.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQIVESESNLKIKRMKCVAMEGLIEEADEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKDTLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(5327..5506)
                     /locus_tag="J1Z28_004133"
     CDS             complement(5327..5506)
                     /locus_tag="J1Z28_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBA7718904.1"
                     /translation="MTEHRGGSGSFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         1497 a         1299 c         1272 g         1445 t
ORIGIN      
        1 aacacggttg caaaaaccgt gcccttaaat attgaatttc tattcagaac actttcttaa
       61 atggtttcac tgaaacgtgt tcatagactc ctgccgctac gtacgggtca gcatcggccc
      121 aggcctgagc tgcttccagc gactcaaatt cagcaataac ggttgagcca gtaaatcccg
      181 cagcccctgg atcgttactg tctaccgctg gcattggacc ggcggtcaac aaacgacctt
      241 catcatgcag taactgtaaa cgtgccaaat gtgccggacg aacggaaagg cgtttttcga
      301 ggctatcagc tttgtcttga gcgtaaataa catacaacac aggcacaact ccttgttcgg
      361 gaaagttgta aagtacgtta tttgaaaggg cgaagatctg caacggaaag atgatgtctt
      421 tgttaagacc atgcataaag taaggtcaat tacgccaaaa atgacatttt cactgatcct
      481 gatcgtcttg ccttattgaa tatgattgct atttgcattt aaaatcgggg cctggttttt
      541 ctactgaaat gattatgact tcaatgaccc ttgatttacc tcgccgcttc ccctggccga
      601 cgttactttc ggtctgcatt catggtgctg ttgtggcggg tttgctctat acctcggtac
      661 atcaggttat tgaactacct gcgcctgcgc agccgatttc tgtcacgatg gttgcgcctg
      721 ctgatcttga accgccacaa gccgttcagc caccaccgga gccggtggta gagccagaac
      781 cggaacctga gccgatccct gaaccgccaa aagaagctcc ggtggtcatt gaaaagccga
      841 agccgaaacc taagccaaaa ccgaagccgg tgaaaaaggt ggagcagcca aaacgcgatg
      901 tcaaacccgt agagtcgcgt ccggcatcac cgtttgaaaa tacggcaccc gcgcgcccga
      961 catcaagtac ggcaacggct gcaaccagca agccggttac cagtgtggct tcaggaccac
     1021 gcgcattaag ccgtaatcag ccgcagtatc cggcacgagc acaggcattg cgcattgaag
     1081 ggcaggttaa agttaaattt gatgtcacgc cagatggtcg cgtggataac gtacaaatcc
     1141 tctcagccaa gcctgcaaac atgtttgagc gtgaggtgaa aaatgcgatg cgcagatggc
     1201 gttatgagcc gggtaagcca ggcagtggga ttgtggtgaa tatcctgttt aaaattaacg
     1261 gcactaccga aattcagtaa gcataaagtc aaaagcctcc gaccggaggc ttttgattat
     1321 tactcaacgg gtaaggcgcg aggttttcct tcgggatcaa ccgcgacata cttaaataat
     1381 gcttctgttg ctttatagcg ttgcccgatt ggttcagacg ccactttttt cacccacact
     1441 tcaatattaa tgctgactga tgtcgtccct ttctggacac agcgtgcata gcagcacacc
     1501 acatcgccga ccgcaaccgg ccgtaagaaa gtcattcctt caacccgcac ggtcactaca
     1561 cgaccatggg caatttcttt cgccagaata gcgccgccaa tatccatttg tgacattaac
     1621 caaccaccaa agatgtcacc attggcattg gtatcggcgg gcatggctaa agtacgtaaa
     1681 acaagatcgc cctgagggac gttatgtgtt gtagacatgg taaaaccgac ttaaaaggaa
     1741 aatcacaggc gcgatgctac tatgattttc ctgaggagaa cagaggagat acagcgccag
     1801 cccctgaagg actggcagtc tggttaggat ttatcttcct gcggcatgtg gcggtagata
     1861 tagataccgc ttaacaatgt gaagattaag gtaagggcgg tcaggccaaa gactttaaag
     1921 ttgacccaaa tattttgcgg cagccagaat gcgatgtaga tgtttgccag accgcaaagg
     1981 ataaagaaaa cagcccaggc cagattcagc ttcgaccata ccgattgcgg cagggtgagt
     2041 tctttaccca gcatccgctg aattagcggc tttttcatca cccattggct gactaacagg
     2101 gcacccgcaa acagggcata aatgacagta accttccatt taataaactc atcattgtgg
     2161 aagaacagcg tcaagccacc gaagacgacc accagaacaa aagtgatcag ggccatcttc
     2221 tcaaccttac gaaagcgaac ccagctatat ataagcacaa tcgccgtggc gacgatgagc
     2281 gccgcagtag ccgcatagat gtcataaatc ttgtaaaacg cgaaaaagac aaccagcggt
     2341 aaaaaatcaa gaaactgctt cattttacga ttccgtaatc aaatgcttaa ggttattggc
     2401 gaatcaacat atacaggcgg aacagataga tgagcaatac ggctgaaatc aggttgctca
     2461 aggtgttcgc cagtacggca ccaatttccg gggttaatgc ggcaaaagaa gaggcaaaaa
     2521 gcagcaacag tgtttttgcc agcaaccagc tcagtactgc gggtgccacc agacgcatat
     2581 tcgcccatgt cagccgcata ctgctacgca tcgaggcaaa aacgcccatt ttgtcctgta
     2641 ccagcatcac cggtgccaga gccagtaaaa tggccatgat aattcccgga acgaccacca
     2701 gcatgatgcc aatctgtact aaaagggtag tcagaaaaat cagaataaat aactttggta
     2761 atatcggcgc actggcacca atagctcgca gcgcactgac tctctgaccc gcagacacca
     2821 gctggataat taatattacg cccccggcga gaatggcgtt accgattaat cctgaaaaag
     2881 tggacgccgc tgaagcctgc agcaaaattt gttgctgttc cggtgacata ttctgaacca
     2941 ggtcgaacaa cccactactg ccgctaacgg gtacgccgtc attgagctgc gcaagctgtg
     3001 catcactggg tgagaaaaca tgccctaaca ccactgtgat aaacgcacat agcaacgata
     3061 ccaacagaat ggtcataaat tgattacgga aaaaatttcc ggtgtcacgg tatacggact
     3121 gcgccgtgat agacatgcac tctccttgag ttttatgcag gtgttaatta gcgggcaatt
     3181 gtaccctggt taagccccgg gtggcagcat caggtcttgt atggaaaagc atatctttgt
     3241 aaagcggagg taattgctgg ccttcaggcg tgggcaaacc gctgaaagtg gcgagcgagt
     3301 taatatttgc gtagcgaaat atataaaaat tgatttaaat cacattaacc aggattttca
     3361 atgcaacttc taaattaatc cagatcaata aagggtgaat taccatatgt aatgtgatct
     3421 gtgtaggatc atttgttacc ccaatgtggg tatattcgtc acgtttttat aaccataacg
     3481 acggagcgga tatgaaaaag ttaacagtgg cggctttggc agtagcaact cttctctctg
     3541 gcagtgcctt tgcgcatgaa gccggcgaat tttttatgcg tgcaggttct gcaaccgtac
     3601 gtccaacaga aggtgctggt ggtacgttag gaagtttggg tggattcagc gtgaccaata
     3661 acactcaact gggcctgacg tttacttata tggcgaccga caacattggt gtggaattac
     3721 tggcagcaac gccgttccgc cataaaatcg gcacccgggc gaccggcgat attgcaaccg
     3781 ttcatcatct gccgccaaca ctgatggcgc agtggtattt tggtgatgcc agcagcaaat
     3841 tccgtcctta cgttggtgca ggtattaact acaccacctt ctttgataat ggatttaacg
     3901 atcatggcaa agaggcgggg ctttccgatc tcagtctgaa agactcctgg ggagctgccg
     3961 ggcaggtggg ggttgattat ctgattaacc gtgactggtt ggttaacatg tcagtctggt
     4021 acatggatat tgatacgact gccaattata aactgggcgg tgcacagcaa cacgatagcg
     4081 tacgcctcga tccgtgggtg tttatgttct cagcaggata tcgtttttaa ttccgcacaa
     4141 aaatgacccc gtaattacgg ggtcaataag gacatggtat aaagggtatt atttcttcgc
     4201 ttctacgcca tcagtttcag agcgaattaa gaatttctca gttgtttgcg gaatatgctg
     4261 aattagccag tcggccatgt gcttttcatc atttaaaatc gcttcgataa tcggaattga
     4321 ggctgtatca ccggcatttt ttgctgctgc taagagtgag gtataacagg cgatttcaaa
     4381 ttgctcgaag acatatccgc taatagagcc tttgactatt tcatcagaag ggaatatacc
     4441 accgattgac tgcccaagtg cagccatttt actcatggaa tctttaatga ctgaacgtga
     4501 aatgtcatta cgatcaagaa tagtttccag ttgaacaatc tggtttttgg tttcactaag
     4561 atgttgttca atacgagcgc gtagttcagg ataattatct atacgactgg ccatggattc
     4621 aagcatagat tcggcttact tttccattgc gtgggcgtca cgtaaccagt catgataatg
     4681 ttcaatacga ttcatgaaaa tgtcctcttt ataaaatgac taaaagttaa agttcatatt
     4741 tcaggcttta ttttcggctt tcttatttac gttattaagg gccagatcag tcaatttgat
     4801 gtcggtggcc ttttcttctt ccagggtatc tttcagaagc ttcgctgctt tacgataacc
     4861 taattgttca gccagcgtcg ctaatgtccc gtaactggca atctcataat gctcgacttt
     4921 ctgtgctgcg gcaatcagtg cggcatcacg cacttcgttt ttctcggtac tttcgatgac
     4981 ctcatcagct tcttcaataa gaccttccat tgccacacat ttcatgcgct taattttcag
     5041 attcgattct gattccacaa tttgatcaat acgttcaatc tgtccatgtg tttcctcgag
     5101 gtgcgcatga aaagcctgac ttaatttttc gtttgatgtt gctcttgcga gttttgccag
     5161 tgcccgggtt aattgttttt ctgcgctgta ggtatctgaa agcaggtgaa taaatacatc
     5221 ttcaatggtc ttcatattca tatttttctc cagcgaaatc actgcgagcc atacggcccg
     5281 cagatgtaat tatctttatc agctgatatt aataattaac ggtgaatcag gattttcctg
     5341 atttattacc accgctttgt tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat
     5401 catttttaaa attaccgccg ctatgctgac cgcctttacg gcctgcgtcg gatgccttct
     5461 cacggtcttc ggcgaaactt cctgaaccac cacgatgttc ggtcatgtta ttt
//
LOCUS       DADSZD010000228         5483 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.230, whole genome shotgun sequence.
ACCESSION   DADSZD010000228 DADSZD010000000
VERSION     DADSZD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.230"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            202..1416
                     /locus_tag="J1Z28_004134"
     CDS             202..1416
                     /locus_tag="J1Z28_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBA7718905.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1483..2766
                     /locus_tag="J1Z28_004135"
     CDS             1483..2766
                     /locus_tag="J1Z28_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7718906.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3018..3935
                     /gene="oxyR"
                     /locus_tag="J1Z28_004136"
     CDS             3018..3935
                     /gene="oxyR"
                     /locus_tag="J1Z28_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBA7718907.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3918..5318)
                     /gene="sthA"
                     /locus_tag="J1Z28_004137"
     CDS             complement(3918..5318)
                     /gene="sthA"
                     /locus_tag="J1Z28_004137"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBA7718908.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHYEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
BASE COUNT         1231 a         1264 c         1490 g         1498 t
ORIGIN      
        1 agtttgttat gtgattattt aacgtgttgt tttaacgata atggctaatt attcaggtgg
       61 tttttgaggg tgttttttct cctcggtaga aatttttatt tttaatgtga tcacggttga
      121 ataatcacct tttctctctt gtatggtagt aggcgtaaaa ttaagtttga gtgtctattt
      181 acaatacagg agattgcaat aatgaaaatt attgctgtgg atgtttttgt tacttgtccc
      241 gggcgtaatt tcgttacgct taaaataacc actgaaagcg gactttgtgg attaggagat
      301 gccacgttaa atggtcgcga attgtcggtc gcttcttatc tgaaagatca tctttgtcct
      361 caattaattg gccgcgatgc cagccgtatc gaagatatct ggcaattctt ttataaagga
      421 gcttactggc gacgtggtcc cgtcacgatg tcggccattt ctgccattga tatggcgttg
      481 tgggatatca aagctaaagc ggcaaatatg ccattgtatc aactgcttgg tggcgcttct
      541 cgtgaaggcg tgatggttta ttgccacacc accgggcgga cgattgatga agtccttgaa
      601 gactatgcaa agcaccagca gatgggcttt aaggcgattc gtgtgcagtg tggtgtaccg
      661 ggtatgcaaa ccacctatgg tctggcgaag ggcaaaggac tggcgtatga accagcaacc
      721 aaaggcctgt ggccagaaga gcagttgtgg tcttcagaaa agtatctcga ttttacgccg
      781 aagttatttg aagcggtgcg taataagttc ggttttaacg aacatctgtt gcatgacatg
      841 caccatcgcc tgacccccat cgaagcggcg cgctttggga ggagcattga ggattaccgc
      901 ctgttctgga tggaagatcc gacgcctgcc gagaaccagg aatgttttcg tctgattcgc
      961 cagcataccg tgacgccgat tgcggtcggt gaagtgttta acagtatctg ggactgcaaa
     1021 cagttaatcg aagagcagct gattgactat attcgcacga cgattaccca tgcaggcggc
     1081 attaccggga tgcgacggat tgccgatttt gcatcgcttt atcaggtacg taccggttcg
     1141 catggccctt cagatttatc gcctgtttgt catgcggcgg cgctgcattt tgacctctgg
     1201 gtgccaaact ttggcgttca ggagtacatg ggctattccg aacaaatgct tgaagtcttc
     1261 ccgcacagct ggcgcttcga tgaaggttat atgcacccag gcgatgaacc ggggctgggt
     1321 atttctttcg atgagaaact ggcggcgaaa tatccgtatg acccggcgta tctccccgtc
     1381 gcacgactgg aagatggcac attgtggaac tggtaatagc ggttcgctta attattttct
     1441 gaatattcat caaatcgatt aattccgctc aaggagctaa aaatggacgt tgatgtgtca
     1501 accagtgttg cgggtaataa accgcaacgg attcgtcgta ttcagacagt gaccctggtt
     1561 ttattattta tggcggggat cgttaatttt ctcgaccgtt cgtcattgag cgtggcaggt
     1621 gaagcaattc gtggcgagtt aggattatcc gctaccgagt ttggtgtttt gctttctgca
     1681 ttttctctgt cttatggttt ttcacaactt ccttccggta ttttgctaga ccgctttggc
     1741 ccacgaatag tgttaggcgc aggcttaata ttctggtcat taatgcaggc attaacagga
     1801 atggttaatt cttttagcca ctttatttta atgcgtatcg gtctgggtat tggcgaagcg
     1861 ccatttatgc ctgcgggagt aaagtcgatc accgactggt atgcgcaaaa agaacgcggc
     1921 acggcgctgg ggatttttaa ctcatctacc gttatcggtc aggctatcgc gcctcctgct
     1981 cttgtattga tgcaactggc atggggctgg cggacgatgt ttgtcatcat cggcgtagca
     2041 gggattctgg ttgggatctg ttggtacgcg tggtatcgca accgggcgca gtttgtcctg
     2101 accgaggaag aacggacata tctctccgcg ccggttaaac cgcgtccaca gttacaattt
     2161 agcgagtggc tggcgctgtt taagcatcgg acaacctggg ggatgatttt aggtttctct
     2221 ggcgtcaatt ataccggttg gctctacatc gcgtggttac ccggttattt gcaggccgaa
     2281 caaggtttca gtctggcgaa aaccggttgg gtggcggcaa ttcctttcct cgcggcggcg
     2341 gtcgggatgt gggttaacgg tattgttgtc gatcggctgg cgaaaaaagg ctatgacctg
     2401 gcgaagacgc gtaaaacggc gattgtctgc ggtttgatga tgtcggcatt aggcacgttg
     2461 ctggtcgtgc aatcttcctc gccagcccag gcggtagcgt ttatctcaat ggcgctgttc
     2521 tgcgtgcatt tcgcgggaac atctgcatgg gggctggtgc aggtgatggt gtcagaaaca
     2581 aaagtggctt ccatcgccgg gattcaaaac tttggcagtt ttgtttttgc ttcctttgct
     2641 ccgatcgtaa ccggttgggt cgtggatacc acacactcgt ttaatctggc gctggttatc
     2701 gcggcctgtg tgacgttcac cggggcgctg tgttacttct ttattgtcaa agatcggatt
     2761 gagtaaccgt tactatcagg ctctcttgct gtgggcctgt agaataaaaa aaagcggatc
     2821 ctggagatcc gcaaaagttc acgttggctt tagttattcg agttgagaaa ctctcgaaac
     2881 gggcagtgac ttcaagggtt aaaagaggtg ccgctccgtt tctgtgagca attatcagtc
     2941 agaatgcttg atagggataa tcgttcgttg ctatgctacc tatcgccgcg aactatcgtg
     3001 gcaatggagg atggataatg aatattcgtg atcttgagta cctagtggca ttggccgaac
     3061 accgccattt tcggcgtgcg gcagattcct gccacgttag ccagccgacg cttagcgggc
     3121 aaattcgtaa gctggaagat gagttgggcg tgatgttgct ggagcggacc agccgtaaag
     3181 tgttgttcac ccaggcggga atgctgctgg tggatcaggc gcgtaccgta ctgcgtgagg
     3241 tgaaagtcct taaagagatg gcaagccagc agggcgagac gatgtccggg ccgctgcata
     3301 ttggtttgat tcccacggtt ggaccgtacc tgctaccgca tattatcccg atgctgcacc
     3361 agacctttcc aaagctggaa atgtatctgc atgaagcaca gacccaccag ttactggcgc
     3421 aactggacag cggcaaactc gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat
     3481 tcattgaagt gccgttgttt gatgagccaa tgttgctggc tatctatgaa gaccacccgt
     3541 gggcgaaccg cgaatgcgta ccgatggccg atctggcagg ggaaaaactg ctgatgctgg
     3601 aagatggtca ctgtttgcgc gatcaggcaa tgggtttctg ttttgaagcc ggggcggatg
     3661 aagatacaca cttccgcgcg accagcctgg aaactctgcg caacatggtg gcggcaggta
     3721 gcgggatcac tttactgcct gcgctggctg tgccgccgga acgcaaacgc gatggggttg
     3781 tttatctgcc gtgcattaag ccggaaccac gccgcactat tggcctggtt tatcgtcctg
     3841 gctcaccgct gcgcagccgc tatgagcagc tggcagaggc catccgcgca agaatggatg
     3901 gccatttcga taaagtgtta aaacaggcgg tttaagccat ttagcgcagc tacccgatag
     3961 gcttccgcca tcgtcgggta gttaaaggtg gtgttgacga agtactcaat agtgttgccg
     4021 ccacctttct gttccataat cgcctgaccg atatgaataa tttcggcagc gcgctcgcca
     4081 aagcagtgaa tacccagaat ctcttttgtt tcccgatgga acaaaatttt cagcgtgccc
     4141 acgttcatgc cgacgatttg tgcacgtgcc agatgtttaa actgggcgcg gcccacttca
     4201 tatggcactt tcatcgcggt cagctgctgt tcggttttgc ctacagagct gatttccggg
     4261 atggtgtaaa tgccggtagg gatatcttca atcagatgtg cgttggcttc acctttcacc
     4321 agcgcctgcg cggcaatgcg cccctggtca taggctgccg acgccaggct cggataacca
     4381 atcacgtcgc ccaccgcgta aacgtgcggc tgtgcggtct gatacatgct gttgaccttc
     4441 agctgtccgc gactgtcagt ttccagccca atgttctgta acgccagcga atcggtatta
     4501 ccggtgcgac cgttggcata gagcagacag tcagctttca gttttttacc cgacttcaga
     4561 tgcatgatca caccgtcgtc acagccttcg atcttctcgt actcttcgtt gtgacgaatc
     4621 actacgccac tgttccagaa gtgataggag agagaatctg acatctcttg atcgagaaac
     4681 gccagcagac gatcgcgggt gttgatcaga tccactttta catccatacc gcggaagatc
     4741 gacgcatatt cacagccgat cactccagca ccgtaaataa gtacatggcg cggttcgtag
     4801 tgcatgctaa gaattgagtc gctgtcataa atgcgtggat gggtgaaatc aacatctgtt
     4861 ggatgatatg gacgagaacc gcaggcaata acaaattttt cagcagttag tgtttcgacg
     4921 ctgccgtccg ggcaatccag cgccaacgta tgctcatcaa caaagcgagc gttcccctgc
     4981 aatatttcac agtgattacg ttcgtaaaat ccctgacgca tgcgcgtttg ttgattaatc
     5041 acgttatcgg catggttaag gatatcggca aaagaagagc ggagcagtcg ggaatggtcg
     5101 ctgtatagtg ggttttgatt gaattctata atgcggctga cggcgtgacg gagagctttc
     5161 gacgggatgg tgccccagtg ggtgcaaccg ccgccaacat tttgataacg ctcgataact
     5221 gcgacgcgcg caccttgctt aaccaggccc atcgcagcgc cttcgccgcc ggggccggaa
     5281 cctattacta tggcatcgta atcgtaggaa tgtggcatgg tagggcttac ctattcttat
     5341 acataaaagc aacagaatgg taacatttta tcgcgggtaa gccaattgat ccccgtcatt
     5401 tatctggcta tatcgtgagc ggcctttgct ttgtcttcct ccttcttatt ttatttattc
     5461 actattaatc gctttttctt atg
//
LOCUS       DADSZD010000229         5431 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.231, whole genome shotgun sequence.
ACCESSION   DADSZD010000229 DADSZD010000000
VERSION     DADSZD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.231"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            271..1491
                     /gene="arcA"
                     /locus_tag="J1Z28_004138"
     CDS             271..1491
                     /gene="arcA"
                     /locus_tag="J1Z28_004138"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HBA7718909.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGIEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
     gene            1502..2434
                     /gene="arcC"
                     /locus_tag="J1Z28_004139"
     CDS             1502..2434
                     /gene="arcC"
                     /locus_tag="J1Z28_004139"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBA7718910.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKGWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            2458..3462
                     /gene="argF"
                     /locus_tag="J1Z28_004140"
     CDS             2458..3462
                     /gene="argF"
                     /locus_tag="J1Z28_004140"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBA7718911.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTQCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            3519..4922
                     /locus_tag="J1Z28_004141"
     CDS             3519..4922
                     /locus_tag="J1Z28_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBA7718912.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLTVLTMPI
                     MAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            5038..>5431
                     /locus_tag="J1Z28_004142"
     CDS             5038..>5431
                     /locus_tag="J1Z28_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HBA7718913.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAG"
BASE COUNT         1392 a         1294 c         1344 g         1401 t
ORIGIN      
        1 aacaatcaat taacaacgaa gaataactct gcgtatattt gcgtattaat gcattatcgt
       61 attttgtttc tttatcattt atcagaaccg catcaaatat tatcaacttc gattattaat
      121 atcaccgccg gtgtgaaagt cgtcacattt tctggaagag ataaactgaa tcattgcatc
      181 tgaaaattac tgaacagacc tgactacaag catttaattt gtttgcagcc atctcctctt
      241 tatttttgaa aaagtgttaa aggaataatg atggaaaagc attatgttgg ttctgaaatt
      301 ggacaactac gcagcgttat gttgcatcgc ccaaatctca gtctgaaacg tctaacacca
      361 tccaattgcc aggagttgtt atttgacgat gtattgtccg ttgagcgggc aggtgaagag
      421 catgatattt tcgccaatac gctgcgccag caagggatag aagttctgtt actcactgat
      481 ttactaacac aaacattgga tatcccggaa gccaaaagct ggttactgga aacacaaata
      541 tcagattacc gactcggccc tacgtttgct actgacgtcc gcacctggct tgcagagatg
      601 tcacaccggg accttgctcg tcatttaagt ggtgggctga cttatagtga aattcccgcc
      661 tcaattaaaa atatggtggt tgatacccac gatattaatg atttcattat gaagccgtta
      721 ccgaatcatt tatttacgcg tgatacctca tgctggattt ataacggtgt atcgattaat
      781 ccaatggcaa aacctgctcg ccaacgtgaa accaataatc tacgcgctat ttatcgctgg
      841 catccacaat ttgctggcgg cgaatttatt aaatatttcg gcgacgagaa tattaattat
      901 gaccacgcca cgttagaagg tggtgatgtg ctggtgattg gtcggggggc cgtgttgatc
      961 ggcatgtctg aacgcaccac gccacaagga atcgagtttc ttgctcaggc attgtttaaa
     1021 catcgtcagg ctgaacgggt aatcgctgtt gagctgccta aacaccgctc ctgtatgcat
     1081 cttgacaccg ttatgactca tatcgatatc gacaccttct cggtttatcc cgaagttgtg
     1141 cgcccggatg ttaattgctg gacgctaaca cctgacggtc acggcggcct gaaacgtact
     1201 caagaaagca cactcctcca tgctatcgaa aaagccctcg gtattgacca ggtacgttta
     1261 atcaccacgg ggggtgatgc ctttgaggca gaacgcgaac agtggaatga cgctaacaat
     1321 gtgctcacgc ttcggccggg agtggtagtg ggctacgagc gcaacatctg gactaacgaa
     1381 aaatatgaca aagcaggcat caccgtttta cccatccccg gtgatgaatt aggccgcgga
     1441 cgtggcggcg cacgttgcat gagttgccct ttgcatcgtg acggtatcta aggaagagat
     1501 catggaaaac aaaccaacac tggttatcgc tctcggcggt aatgccctgc ttaaacgagg
     1561 cgaaccactc gaagcagaaa tccaacgtaa aaatatcgat cttgcggcta aaactatcgc
     1621 ccaactgact caacactggc gggttgtact ggttcacgga aatggcccac aagttggact
     1681 tctggcactt caaaatagcg cctatgccca tgtcgctccc tatccactcg acattctggg
     1741 ggccgaaagc caggggatga tcggctacat gctacaacag gcgctgaaaa atcaactacc
     1801 acaacgtgaa atcagcgtgc tgctgacgca ggttgaagtg gatgccaacg atcccgcctt
     1861 cagtaaccca actaagtaca tcggcccaat ttatgaccac gctcagactc aggtactaca
     1921 ggcagaaaaa ggctgggttt tcaaagcaga tggtcactct ttccgccggg tcgtaccttc
     1981 tccacaacct aaacgcatcg tcgagcggga tgccatccag acactgatcg cccacgatca
     2041 tctggtgatc tgtaatggtg gcggtggtgt tccggtggtt gaaaaagccg atggatacca
     2101 tgggattgaa gcggttatcg acaaagatct ttcagccgcg ctactggcaa gtcagatcca
     2161 tgctgacgcc ttgttgatcc tcactgatgc ggacgcagta tatctcgact ggggcaagcc
     2221 aacccaacgc ccgctggcac aggtcacgcc ggaattactc aacgaaatgc agtttgatgc
     2281 cgggtctatg gggccgaaag tcaccgcctg cgcaaagttt gttagtcagt gccggggcat
     2341 tgcagggatc ggctctcttg cagatggtcc ggaaattctc gccggagaca aaggcactct
     2401 tattcgcctt gatacccccc ataaccacgc ttgatccatt tttataagga catccccatg
     2461 gctacttcac tgaaaaaccg taattttctt aagctgcttg attacacccc agcagagatt
     2521 caatacctga ttgatttggc tatcaatctt aaagccgcca agaaatctgg aaacgaaaaa
     2581 caaacgctgg tcggtaaaaa cattgcgttg atttttgaaa aatcctctac ccgtacccgc
     2641 tgtgcctttg aagtcgcggc ctttgatcaa ggtgcgcagg tgacttacat cggccccagc
     2701 ggctcacaaa tcggccataa agaatcaatg aaagataccg ctcgcgtact cggacgcatg
     2761 tatgacggta ttgagtatcg cggttatggt cagaatatcg ttgaagaatt aggtgagttc
     2821 gctggtgttc cggtatggaa cggactgacc aatgaatttc acccgacaca gatcctcgcg
     2881 gatttaatga ccatgctgga acatgcgcca ggcaaaacgc tgccagagct gagctttgcg
     2941 tatcttggtg atgcccgaaa taacatgggg aactcgctga tggttggtgc tgcaaaaatg
     3001 gggatggata tccgtctggt ggcacctaaa tctttctggc cagatgaagc gctggtaaca
     3061 caatgccgtg agatcgccag cgtgactggg gcgagaatca ccctgacaga ggatgtggaa
     3121 gaaggcgtat acgacgttga ttttctctat acggatgttt gggtttcgat gggagagcca
     3181 aaagaagcct gggcagaacg agtgagtctg atggcacctt atcagatcaa tcaacaggtg
     3241 attactgcca cgcgtaaccc tgaagttaaa tttatgcact gcctgcccgc cttccataat
     3301 gagcacacca cagtaggacg cgagatcgag atggcctacg gcctcaaagg actggaagtt
     3361 actgaagagg tttttgaatc ggcccactct attgttttcg atgaagcaga aaaccggatg
     3421 cacaccatta aagcggtgat ggtagcaact ctcggcgact aaccctgacg ggcatgcgtc
     3481 gacgagtagc gtatgcccgt gattatggag atcacattat gggtaaattc aaatttccca
     3541 ccgcttatac catcttgttc gtgctgattg cgttagttgc ggcaatgacc tggattgtac
     3601 ctgcgggaaa atatcagatg gtgacaaatg ccgcactggg aaaggaagtt cctgttgcag
     3661 gcacttacgc acctgttgag gcgcaaccac agggtattac ggctgtcctg ttagcgccca
     3721 tcgacggctt gtacaaccat caaacctaca ctgcgggagc gattgatgtc gcgctgtttg
     3781 tcctgatcat cggtggattt ttgggagtag taaataaaac cggggcaata gatgccggta
     3841 tcgaacgagt gacaactcgc cttaatggtc gggaagagtg gatgattccg atcctgatgg
     3901 cgctcttcgc tgctggcggt actatttatg ggatggcgga agaatcactc cccttctaca
     3961 cgttgctggt gcccgtcatg atggctgccc gctttgatcc tctggtcgct gccgccacag
     4021 tgttattggg atcgggcatt ggcacgctgg gctcaaccat taacccattt gctaccgtca
     4081 ttgctgccaa cgccgcaggg atccccttta ctcagggcat tttgctgcgt gttattttgc
     4141 tgttagtagg ctacgtgatt tgtgtttact gggttatgcg ttatgcccgc aaagttcgca
     4201 acagtccgga aagctctatc gtggcggata agatggctga aaaccaggcg cattttctgg
     4261 gaaatcgttc agaaacgatg ctggaattta cccccacgcg caaagcgatc ctgatacttt
     4321 ttgctgcatc ctttgccttt atgatttacg gcgtagcggt actgggttgg tggatggcgg
     4381 aaatctctgc tgtctttctg gcagctgcgg tcatcgtggg tgtcattgcc cgcatgggcg
     4441 aagaaacctt caccagtacc tttattgacg gcgcgcggga tctccttggt gtggcactga
     4501 ttatcggcat cgcccgcggt attgtggtcg tgatggataa cggcatgatt acgcacacaa
     4561 ttctgcacag tgctgagaat ctggtttcag ggctttccac cactgttttc atcaacgtga
     4621 cctattggct ggaagtattg ctctcttttc tggttccctc atcttccggc cttacagtgt
     4681 taacgatgcc aataatggct ccactcgctg attttgctca tgtacaacgc gatctggtgg
     4741 tgaccgctta tcagtctgct tccggggtgg ttaatttgat tacgccaacc tctgcggtag
     4801 tgatgggcgg tctggcgatt gcccgtgtac cctgggtccg ttatctgaga tgggtagcac
     4861 cgctactgct gattctcacg atgttgaata tgattgtgct gagtatcgcc gcgatgattt
     4921 aacgcgacag gccgcttatc aaagcggcct tcaaatctgg tgtagtgata aatgaaccac
     4981 tctcacatta taaaatgtga aaaatatcat caggtaataa taaataaaag gtgcattatg
     5041 aaggaatacg atgattactc cgccaaagaa aagaagcaac tggcggtctg tcagcgcctg
     5101 attaccgaaa aaagttatct ttcacaggag gaaatccgcc gcgatttgca aaaccatggc
     5161 tttgacagta tcagtcagtc aaccgtttca cgactactga aattgctggg agtcataaaa
     5221 attagaaata caaaaggaca aaaaatttat tcggtgaatc ctcaattact tcccacacct
     5281 gacgcaggtc gatccgtcgc tgaaatggtc ctgagtgtcg agcataatgg tgaatttatc
     5341 cttatccata ccgtcgccgg atatggtcgg gcagtagcgc gaatccttga tttccatgct
     5401 ttgccagaaa ttcttggcgt gattgctggc a
//
LOCUS       DADSZD010000230         5331 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.232, whole genome shotgun sequence.
ACCESSION   DADSZD010000230 DADSZD010000000
VERSION     DADSZD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.232"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(73..1356)
                     /gene="aroA"
                     /locus_tag="J1Z28_004143"
     CDS             complement(73..1356)
                     /gene="aroA"
                     /locus_tag="J1Z28_004143"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBA7718914.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVHHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            complement(1427..2515)
                     /gene="serC"
                     /locus_tag="J1Z28_004144"
     CDS             complement(1427..2515)
                     /gene="serC"
                     /locus_tag="J1Z28_004144"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBA7718915.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2714..3406)
                     /locus_tag="J1Z28_004145"
     CDS             complement(2714..3406)
                     /locus_tag="J1Z28_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBA7718916.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3536..5296
                     /locus_tag="J1Z28_004146"
     CDS             3536..5296
                     /locus_tag="J1Z28_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HBA7718917.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1375 a         1364 c         1258 g         1334 t
ORIGIN      
        1 gaaaaaaaac gccggcaatg tgccgacgtc tttgaaaaga aaggattgtc tatgttatcg
       61 cccgttattc attcagccgg gttgacttat ccgcgccaat tgctcgaaat agtctggaaa
      121 agttttagcg gtacattttg gatcaagaat cgtcacaggc gtatctgata atgcgaccag
      181 cgagaaacac atcgccatcc ggtgatcatt ataagtggcg atctcagcaa atttcagttt
      241 ttccggtggc gtaatgcgaa tgaaatcatg cccctcttcg acttccgcac cgactttacg
      301 cagttctgtt gccatcgcaa acaggcgatc ggtctcttta acacgccagt tatagatatt
      361 gcgcagcgtg gtggtgcctt ttgcaaataa tgccgcggtg gcaatggtca tcgccgcatc
      421 gggaatatgg ttcatatcca tatcaatggc gttcagttca ccacgcgtgc aggaaatata
      481 atcatcgccc cagcaaatgg tcgcgcccat tttttccaga acatcagcaa agcgaatatc
      541 accctgcata ctgttgcgtc caatgccggt cacttttaca gtgccgcctc tgattgctgc
      601 tgctgccagg aaataagaag ccgaagatgc atcgccttca accaaataag tacccggaga
      661 ctggtaagac tgcccccctt ttacgacaaa ttgttgatag tgctgatttt caatttcaac
      721 accaaacgtc ttcatcagat tgagtgtgat gtcgatataa ggtttagaaa ccagatcgcc
      781 tttaatacga atcaccgtat cttccggcgc aagaggtgca gtcattaaca gtgcggtgag
      841 gaactggctt gaaacggagc catcaacatc aacgttgccg ccagtaaagc cgccctgtaa
      901 acgcaatggc ggataatttt cttgttccag gtaagtgatc ttcgccccgc cctggcgcag
      961 cgcatccacc agatgaccaa tcgggcgttc tttcattcgc ggttcaccgg tcagtacaat
     1021 atcattgcta cccaggcaaa gtgctgccgc cagcggacgc attgccgttc cggcgttacc
     1081 gaggaacaac tcccgggcac tttctgcgtg taatggacca ccgttaccga taatttcgca
     1141 acgcgtacga tcggctgaaa gcgtatagct tacccctaac gctgttaatg cattcagcat
     1201 atggtgcacg tcatcgctat ccagcagatt ggttaatact gttttgccgt gtgctaatgc
     1261 cgccagcaat aaagcgcggt tagaaacgct cttggaaccg ggcagattaa tagtgccatc
     1321 gacacgagcg atgggttgta acgttaggga ttccatgaac tcaactctct acaacagaaa
     1381 taaaaacccc acaggctggc tgtggggatt atgcaaaatt tcgacattag ccgtgacgac
     1441 gttcgaactc aaccatgaag tctgtcagtg ctttaacacc ttccagcggc atggcgttat
     1501 aaatagacgc acgcattccg ccgaccactc ggtgaccttt cagcgcatgt aagcccgcag
     1561 cgaaagactc ttcgaggaac aatttatcaa gcgcgctatc cgccaactgg aacggcacgt
     1621 tcatccgcga acggttagct ttcgccacat cattgcggta gaaatcgctg ttatcaatca
     1681 ccccatacag cagttctgct ttttgctgat tgatcttatc cattgcagcc acaccgccgt
     1741 tcgctttcag ccatttgaag accaggccag ataagtacca ggcaaatgtc ggcggcgtgt
     1801 tgaacatgga atcgttatcg ttgaggatgg agtaatccag aatcgatggg caggcgatat
     1861 tcgctttgcc cagcaaatct tcacgaacga tgacgattgt caggccagcc gggccgatat
     1921 ttttctgcgc gccagcgtaa attacaccat aacggctgac atcaatcgga cgggaaagaa
     1981 tggttgaaga gaagtcagcg gcgaccacca cgtctttacc gaagtctggt gtttcatcga
     2041 tagcgatacc gtcaatggtt tcgttcgggc agtaatgcat ataagcagca ttatcagaga
     2101 gttgccattc actcattggc ttaactgcgc gaagaccatc aacggtcact ttcgcatcaa
     2161 acacatttgg tgtgcagtat tttttcgctt ctttgatagc acttgctgcc cagtaacccg
     2221 catcaacata atctgcagtg gttttatcac caagaatatt cagcggtaca gcagcaaatt
     2281 gaccgcgacc accgccatgg cagaacaata ccttgtagtt ggaggggacg ttaagaagat
     2341 cgcgaaaatc cttctcggct tcctctgcaa cctgaatgaa ctctttgcca cggtgactca
     2401 cttccatcac cgacgtacca agaccgttcc agtcgcgcag ttcctgttga gcctgtttaa
     2461 gcacctctgc cggtagcatt gccggaccag aactaaaatt gaagatttga gccatttccc
     2521 ctcaccacgt tgcgttgcca ttaacccgcg gtcaatcaat tgcgaccaca ggcaatacat
     2581 tattcctgtg gctatcggtt ttatcattca gtgacacgca tcgcaatgag taaaaccgtt
     2641 gagggcaaaa atgaggcacg cgtcacacaa aagaatctct cggattattt tcaaaaaacc
     2701 gatgttttgg ctgtcaggtc acccgtttag cccgacttgc cttcgtccat tctggatttg
     2761 cacagcgcgg acataattgt ttttcccccg cgttcatatg aatgatttca ctgcaacgga
     2821 tgcaggcata gtccgccgac tccggcacca ctttgtaacg ctgagtacag tcactgggta
     2881 aaataagtaa tcccggtttc accttgtcat cttcaaagaa ataaacactg atttgcccgt
     2941 tggtttcgag aatcgccaga cgtacctgcc ccaactgctc tacaccgcgc aatcgtagct
     3001 ccataaagaa ctcaaattcc gtcatgttgg agttattgag tttcgaccag gccagctcgc
     3061 catcttcaat aatgacaacc ggcttgcctt ccagaagatc ttccagtttt tcactgtgcg
     3121 ccatcaacca cattaccagg cggtataaca acgccagagt aataaagacg ataagtaccg
     3181 ggaccatcgg cacatcatca taaaacgcca catctcccgc cgctgaaccc agtgtcagaa
     3241 tgattaacac ttcaaacagc gacatctgcc gcacaccgcg tcttccggtc attttgagga
     3301 acaaaaagac cagtacaaag gtataaagac tacgtagtgc aacttcgcca aggaaatcaa
     3361 aaggcacttt atcgaatgcc atacggtgga gatcaaacgc cttcattttt atgctcttat
     3421 cgtagttaat ccgctaaaag catagctaac agttcatcag cgtggcggcg gtgatagcac
     3481 ctttgagata aaacccgtat tattgcgcgc tttccgtacg actaaagtga ttttcatgac
     3541 gcaaacattt atccccggca aagatgccgc tctggaagat tccatcgcac gcttccagca
     3601 aaaactttca gacctcggtt ttcagattga agaggcctcc tggctgaatc ccgtgcctaa
     3661 tgtctggtct gtacatattc gcgacaaaga gtgcgcactg tgttttacca acggtaaagg
     3721 cgcaaccaaa aaagcggcgc tggcttctgc cctcggtgaa tatttcgagc gtctctcaac
     3781 caactacttt tttgcggact tctggctggg cgaaaccatc gccaacggcc cattcgtgca
     3841 ttatcccaac gaaaaatggt tcccactgac cgaaaatgac gatgtaccag aagggctgct
     3901 cgatgaccgt ctgcgcgcat tttacgatcc ggagaatgaa ctgaccggta gtatgctgat
     3961 tgacctgcaa tccggtaacg aagatcgtgg tatttgcggt ctaccgttta cgcgccagtc
     4021 cgacaatcag accatttata ttccgatgaa tatcattggt aacttgtacg tttctaacgg
     4081 tatgtctgct ggcaataccc gtaacgaagc acgcgttcag gggttgtccg aagttttcga
     4141 acgttacgtg aaaaaccgca ttattgctga aagcatcagc ttgccggaaa tcccggcaga
     4201 cgtgctggca cgttatccag cagtagttga agcgatcgaa acgctggaag cggaaggttt
     4261 cccgatcttc gcttatgacg gttcgcttgg cggccagtat ccggtgattt gcgtggtact
     4321 gttcaatcct gctaacggta cctgctttgc ctcattcggt gcgcatcctg attttggcgt
     4381 tgcactggaa cgtaccgtga ccgagttgct gcaaggtcgt ggcctgaaag atctggatgt
     4441 gtttactcca ccaaccttcg atgatgaaga agtcgctgaa cataccaacc tcgaaacgca
     4501 ctttatcgat tccagcggtt taatctcctg ggacctgttc aagcaggatg ccgattatcc
     4561 gtttgttgac tggagcttct ccggcaccac ggaagaagag ttcgccacgc tgatggctat
     4621 cttcaaaaag gaagataaag aagtttatat tgccgattac gagcatctgg gtgtctacgc
     4681 ttgccgtatt atcgtgcctg gcatgtccga tatttatccg gctgaagatc tgtggctggc
     4741 gaataacagt atgggcagcc atttacgtga aacgattctt tcgctaccag gtagcgagtg
     4801 ggaaaaggaa gattacctga acctcatcga gcaactggat gaagaaggtt ttgatgactt
     4861 tacccgcgtg cgtgaactgt tgggtctggc gaccgggtcg gataacggct ggtacactct
     4921 gcgtatcggt gaattaaaag ccatgctggc gctggctggt ggcgatctgg aacaggctct
     4981 ggtctggacc gaatggacga tggagtttaa ctcatcagta ttcagcccgg aacgcgccaa
     5041 ttattatcgc tgcctgcaaa cgttgttatt actggcgcag gaagaagatc gccagccgct
     5101 gcaatatctg aatgcgtttg ttcgcatgta cggcgcagat gccgtggaag ccgccagtgc
     5161 ggcaatgagc ggcgaagcgg cgttttatgg cttgcaacca gtagatagcg atctgcacgc
     5221 gtttgctgca catcagtcgt tgttgaaggc ctacgaaaag ctgcaacgcg ccaaagcagc
     5281 attctgggca aaataaaatc aaatagccta cgcaatgtag gcttaatgat t
//
LOCUS       DADSZD010000231         5297 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.233, whole genome shotgun sequence.
ACCESSION   DADSZD010000231 DADSZD010000000
VERSION     DADSZD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.233"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            166..699
                     /locus_tag="J1Z28_004147"
     CDS             166..699
                     /locus_tag="J1Z28_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBA7718918.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVAISESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSGQSQPEPQAEAGTKKGGAKTKGRGRKAAQTE
                     PQPPSEPEYNFDDDIPF"
     gene            757..990
                     /locus_tag="J1Z28_004148"
     CDS             757..990
                     /locus_tag="J1Z28_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBA7718919.1"
                     /translation="MREYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFKDGAGYWMNHAIRDFGILK"
     gene            1054..3045
                     /locus_tag="J1Z28_004149"
     CDS             1054..3045
                     /locus_tag="J1Z28_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBA7718920.1"
                     /translation="MSVTESKTKTERKSSRKPSKEQETALSALSALSALSALLAQTAE
                     VSVPLASLIKSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHALPGDRYGVAAGGR
                     RLAALNMLAERNILPADWPVRVKIIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQ
                     EGKTPAQIGDLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQ
                     VQVFEAACQSGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSD
                     DEGGYVDCVALDVALLEKLQAVAEFLREAEGWEWCAGRMEPVGFCREDAGTYRSLPEP
                     EAVLTEAEEERLNELMARYDALENQCEESDRLEAEMKLMRCMATVRAWTPEMRAGSGV
                     VVSWRYGNVCVQRGVQLRSEDDVADDTERTEQVQEKASVEEISLPLLTKMSSERTLAV
                     QAALMQQPDKSLTLLAWTLCLNVFGSGAYSKPAQISLECKHSSLTSDAPSGKEGAAFL
                     ALMAEKARLAALLPEGWARDMTAFLSLSQEVLLSLLGFCTACSIHGVQTREYGHTSRS
                     PLDPLETAIGFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAA
                     EHAEFHMKDNRWVPGWMCALRPQAETETTEYRDNQADAA"
     gene            3100..3534
                     /gene="psiB"
                     /locus_tag="J1Z28_004150"
     CDS             3100..3534
                     /gene="psiB"
                     /locus_tag="J1Z28_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBA7718921.1"
                     /translation="MKTELTLNVLQAMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVNDIIHILMAEGGQV"
     gene            3531..4250
                     /locus_tag="J1Z28_004151"
     CDS             3531..4250
                     /locus_tag="J1Z28_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBA7718922.1"
                     /translation="MSARSQALVPLSAEQQAAWWAVAETEKRRHQGNLLAEYPYAGAF
                     FRCLNGSRRMSLSDLRFFMPSLTAEELRGNHLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRLRVRDRSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWSERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4247..4561
                     /locus_tag="J1Z28_004152"
     CDS             4247..4561
                     /locus_tag="J1Z28_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HBA7718923.1"
                     /translation="MKWRYSLRWKRPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            4768..4929
                     /locus_tag="J1Z28_004153"
     CDS             4768..4929
                     /locus_tag="J1Z28_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBA7718924.1"
                     /translation="MKLPGNALIWCVLIVCCTLLIFTFLTRNRLCEIRLKDGYREVTA
                     SMAYESGGK"
BASE COUNT         1176 a         1398 c         1662 g         1061 t
ORIGIN      
        1 tgcgtcaggc cgtggcagtg atgccccttg cggcgaaggt tctcaaaaag gtgatggtga
       61 ataccggagg gaacaaaagg caggtccccg caaaacgtca aaaccgcggc tcccgaaact
      121 caaaagatga tgggggctga aaacatcaac agaaggagac acatcatggc agttcgtggc
      181 attaacaagg tcattctcgt aggccgcctg ggaaaagatc cggaagtccg ttacatcccc
      241 aacgggggcg cagtggcaaa cctgcaggta gccatatcag aaagctggcg tgacaaacag
      301 acgggggaga tgcgggagca gacagaatgg catcgtgtgg tgctgttcgg caagctcgcg
      361 gaagtggctg gtgaatatct gcgcaagggc gcgcaggtct acatcgaagg tcagcttcgc
      421 acccgtagct gggaggataa cggaatcacc cgttacgtca ccgaaattct tgttaagacc
      481 acgggcacca tgcagatgct gggacgtgcc gcaggtgctc agactcagcc ggaagagggg
      541 caacagttca gcggtcagtc tcagccggaa ccacaggcgg aggccggtac gaaaaaagga
      601 ggcgcaaaaa cgaaaggccg tggacgtaag gccgcgcaga cggagcctca gccgccgtct
      661 gaacccgaat ataacttcga tgacgacatc ccgttctgat cgggctgact gtgataaccg
      721 ccccgttctg tgcggggcat caccggagag atgaggatga gagaatattt cagaatactt
      781 caggggctgc ctgacggtcc ttttacccgc aaacatgccg aagctgttgc cgcacagtac
      841 cggaacgtct ttatcgagga tgaccacggc gaacagtttc gcctggttgt ccgtaataac
      901 ggaacaatgg tctggcgtac ctggaatttt aaggacggtg ccgggtactg gatgaaccat
      961 gccatccgtg atttcggcat tcttaagtaa gagaggtgcc ggacgggaac gcgtccggca
     1021 gacatacaac catcccggtc aaaggagaaa tatatgtcag ttacagagtc taagacaaaa
     1081 acggagcgta aatccagccg taaaccgtca aaagagcagg agactgccct gtctgccctg
     1141 tctgccctgt ctgccctgtc tgccctgctg gcgcagacgg cggaagtgag cgtgccgctg
     1201 gcctcactga ttaaatcacc actgaatgtg cgcacggtgc catattctgc ggagtctgtc
     1261 agcgaactgg cggagtccat taaaggcgtc ggcctgctgc agaatctggt cgtgcatgcc
     1321 ctgccaggtg accgctacgg tgtcgccgca ggcggtcgcc gactggcagc actcaacatg
     1381 ctggcagagc gcaacatcct tccggctgac tggcctgtac gcgtgaaaat tattccgcag
     1441 gagctggcga cggctgcatc gatgaccgag aacggtcatc gtcgggatat gcaccctgcc
     1501 gaacagattg ccggattccg cgcaatggcg caggaaggca aaacacctgc acaaatcggt
     1561 gatttgctgg gctattcacc ccgccatgtc cagcgaatgc tgaaactggc agatcttgcg
     1621 cctgtcatcc tcgatgcgct ggcagaagac cgcatcacca ccgaacactg tcaggcgctg
     1681 gcactggaga acgacaccgc gcgtcaggtg caggtgtttg aagctgcttg tcagtcggga
     1741 tggggtggaa aaccggaagt gcagaccatt cgtcgtctgg tgaccgaaag tgaagtggcg
     1801 gtggcgggga acagtaaatt ccgcttcgtg ggggctgatg ccttctcgcc agatgaactg
     1861 cgcaccgatt tgttcagcga cgacgagggt ggctatgtgg actgtgttgc gctcgatgtc
     1921 gccctgctgg aaaaactcca ggctgtcgca gagttccttc gggaagccga aggctgggaa
     1981 tggtgcgccg gacgtatgga gcctgttggt ttctgtcgtg aggatgccgg aacataccgc
     2041 agtctgccgg agccggaagc ggtgctgacg gaggcggaag aagaacgtct gaacgaactg
     2101 atggcacgtt acgatgctct ggaaaaccag tgtgaggaat ccgaccggct ggaagcagaa
     2161 atgaagctga tgcgctgcat ggcgacggtc agggcgtgga cgccggagat gcgtgccgga
     2221 agcggtgtgg tggtgtcctg gcgttatgga aacgtatgtg tccagcgtgg tgtgcagttg
     2281 cgcagtgagg atgacgtggc tgacgacact gaacgcacgg aacaggttca ggagaaagcc
     2341 tccgtggagg aaatcagtct gccgttgctg acgaaaatgt cctcagagcg cacgctggca
     2401 gtccaggcgg cactgatgca gcagccggac aaatccctga cactgctggc atggacgctc
     2461 tgtctgaatg tgtttggcag cggagcgtac agtaaaccag cacaaatcag cctggaatgt
     2521 aaacattctt cgctgaccag cgatgcgcca tcggggaagg aaggtgccgc attcctggct
     2581 ttgatggcag aaaaagcccg tcttgccgcc ctgctgccgg aaggatgggc acgggacatg
     2641 acggcgttcc tgtcactcag ccaggaggta ctgttatccc tgctcggttt ctgcactgca
     2701 tgcagcatcc acggcgttca gacccgtgag tatggtcaca cgtcacgcag tccgcttgac
     2761 ccgctggaaa ccgccatcgg ctttcacatg cgcgactggt ggcagccgac aaaagcaaac
     2821 tttttcgggc acctgaaaaa gccgcagatt atcgcagccc tgaatgaagc cggactgtcc
     2881 ggtgccgcgc gggacgcaga gaagatgaag aaaggtgatg cggctgaaca tgcagagttc
     2941 catatgaaag acaaccgctg ggtgcctggc tggatgtgtg cactacgtcc acaggcagag
     3001 acggaaacca cggaataccg tgacaaccag gccgatgccg cctgatgaac aaccacaccg
     3061 ccccgccgga gacggggcag cagcaaggga gataccgtga tgaaaactga actgaccctg
     3121 aatgtattac aggccatgaa cgcacaggaa tatgaagata tccgggctgc cggaagtgat
     3181 gaacgccgtg agctgacaca cgccgtgatg cgggaactgg atgcgccgga caactggacg
     3241 atgaatggcg agtacggcag cgagttcggc ggctttttcc ccgtccaggt ccgtttcacg
     3301 cctgcccacg aacgttttca cctggcgtta tgttcaccgg gtgatgtctc tcaggtctgg
     3361 gtgctggttc tggtgaatgc cggtggtgag ccgtttgcgg tggttcaggt gcagcggcgg
     3421 tttgcaccgg aagccgtcag ccattcactg gcactggcgg cgtcactgga tgcgcagggg
     3481 tacagcgtta acgacatcat tcatatcctg atggcggaag gaggtcaggt atgagcgcac
     3541 gttcacaggc actggttccc ctcagcgcag agcaacaggc cgcatggtgg gcggtggcgg
     3601 agacggaaaa acgccgccat cagggaaact tgctggcgga atatccgtat gcgggggcgt
     3661 ttttccgctg tctgaacgga agccgcagga tgtcgttgtc tgacctgcga tttttcatgc
     3721 cgtccctgac cgcagaagaa ctgcgcggca accatcttca gtggctgtat gccatcgatg
     3781 tgctgattga aacacagggc gaggtctgcc tccttcccct tcccggcgat gcagcagagc
     3841 ggctgttccc gtcggtgcgg ctccgtgtca gggatcgcag tcgccacaaa tccgcactgg
     3901 tcatgcagaa gtacagccgc cagcaggcac gggaggcaga gcagaaagcc cgtgcgtacc
     3961 aggcgctggt tgctcaggct gaaatcgaac tggcgttcca ttcaccggaa acagtcggaa
     4021 gctggcatgc acgctggagt gaccgggtgg cagagcatga cctggaaacc ctgttctggc
     4081 agtggagcga gcgttttccg tcactggccg gaatggaacg ctggcagtgg caggatatgc
     4141 cgttctggca ggtcatcgcg gaagccagtc ttgcagcaag agaggccggt cacgctgtcc
     4201 gtgagatgga gcggtggatg gtgccgaaca aactccggga ggcggcgtga agtggcgtta
     4261 ctcactgcgc tggaaacggc cttatccctg tcctggcgag catgaactgg tttcagaagt
     4321 ggtggaggcg gggcagcctg caccggcatc cgttatgtca cgctgggttg ccggagcagg
     4381 gtatgcggtc tgtctggatt ttatttctga ccgcccggtg cgccgctgga gcgaagaacg
     4441 taaggctgcg gtacgacggc ggaatctgga gaaacgtatc aacaggcatg caccactgtt
     4501 tgcggatgaa ctgattgccc gggaactggc tgaacgacct gattattttc agggaaagtg
     4561 agtgtggtca gcgtgcaggt atatgggcta tgatgtgccc ggcgcttgag gctttctgcc
     4621 tcatgacgtg aaggtggttt gttaccgtgt tgtgtggcag aaggacaaaa gccccgtagt
     4681 taattttcat taacccacga ggcccctgca tgtctaacca acaccagtat ggcctcttac
     4741 cgtgcaaatt gcaaggagga gtaaaccatg aaactaccgg gaaacgccct tatctggtgc
     4801 gtattaatcg tgtgctgcac gttgttgata ttcacattcc tgacccggaa tcgcctgtgc
     4861 gaaatccggc tgaaggacgg atacagggag gttacggcaa gcatggctta cgaatccggc
     4921 ggtaagtagc aacccggagg cgggcgcagg cccgcctttc cggaactgat gcgggtctga
     4981 ctgcactgaa gcgcctttat gaagggactg ccggttcgtc cattttccgg cagtcccttt
     5041 ttccttgctg gcattgtgcg ggcatgaaca accaggcagc caggaaggcc gtttccctcc
     5101 gggaacgggg atgaacgcgc gcctgcggcg cccgttcttc tcccccgcct tctctggtta
     5161 tggccagtca gaaatcacat caccttccct gtgattattc tcttttccct cctgttctga
     5221 ttctgattac tgctgctctg attcctgtca gttctgttgt tctcctgcct gtgccttttc
     5281 cgggcgtccc cctggcg
//
LOCUS       DADSZD010000232         5288 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.234, whole genome shotgun sequence.
ACCESSION   DADSZD010000232 DADSZD010000000
VERSION     DADSZD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.234"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1205
                     /gene="emrY"
                     /locus_tag="J1Z28_004154"
     CDS             <1..1205
                     /gene="emrY"
                     /locus_tag="J1Z28_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBA7718925.1"
                     /translation="RVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPI
                     CGPILGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLV
                     LGVGGLQIMLDKGRDLDWFNSSTIILLTVVSVISLISLVIWESTSENPILDLSLFKSR
                     NFTIGIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGR
                     YGNKIDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTT
                     ISFSGLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPV
                     FNSSSQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKP
                     PFTAKGVG"
     gene            complement(1313..2641)
                     /gene="dsdA"
                     /locus_tag="J1Z28_004155"
     CDS             complement(1313..2641)
                     /gene="dsdA"
                     /locus_tag="J1Z28_004155"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBA7718926.1"
                     /translation="MENAKMNSLIAQYPLVEDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAAAGGIIESELVAIPAMQKRLEKEYHQPIAGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVNADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLSWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(2659..3996)
                     /gene="dsdX"
                     /locus_tag="J1Z28_004156"
     CDS             complement(2659..3996)
                     /gene="dsdX"
                     /locus_tag="J1Z28_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HBA7718927.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTVLLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVIALAGTFLLSFII"
     gene            4214..5149
                     /gene="dsdC"
                     /locus_tag="J1Z28_004157"
     CDS             4214..5149
                     /gene="dsdC"
                     /locus_tag="J1Z28_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HBA7718928.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLAHHFLMDEEILPVCSPEYAQRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
BASE COUNT         1424 a         1243 c         1240 g         1381 t
ORIGIN      
        1 ttagagtcgt tcaggggtta atggcggggc cgttaattcc actgtcacag agtttattat
       61 taaggaatta tccaccagaa aaaagaacat ttgctctggc attatggtca atgaccgtga
      121 ttatcgctcc gatatgtggg ccgatattgg gcggctatat ttgcgataac tttagctggg
      181 gttggatatt tttaatcaat gtccctatgg ggattatcgt cctgacatta tgcttaacct
      241 tacttaaagg aagagaaact gagacttcac cggtcaaaat gaatctacca ggactgaccc
      301 tgttagtgct cggtgttggt ggcttgcaaa ttatgcttga taaagggcgc gatctggatt
      361 ggttcaactc gagtacaata atactactaa cagtagtatc agttatttct ctgatctctt
      421 tagtcatttg ggagtcgacc tcagagaatc cgattcttga cctcagtttg tttaagtccc
      481 gtaatttcac cattggtatt gtgagtatca catgcgcgta tttattttac tctggagcga
      541 tagttcttat gccgcagtta ctccaggaaa cgatggggta taatgcgata tgggccggac
      601 ttgcctatgc gcccatcggt atcatgccgc tattaatatc acctttgata ggacgttatg
      661 gcaacaaaat tgatatgcgg ttgttggtga catttagttt tttgatgtat gcggtttgct
      721 attactggcg ttctgtgaca tttatgccaa cgattgattt tacaggcatc attttgccac
      781 agttttttca gggattcgcc gttgcctgtt tctttttacc cttaacaacg atttcgtttt
      841 caggcttgcc agataataaa tttgccaatg cctcgagtat gagtaatttt tttcgcactt
      901 tgtcaggatc agttggtacg tcgttgacaa tgacactgtg gggacgccgc gaatcgttac
      961 accatagtca gttgacagca accatcgatc aatttaaccc cgtgtttaat tcatcgtcac
     1021 aaattatgga taaatactat ggttcgcttt caggagttct taatgaaatt aataatgaaa
     1081 taactcagca gtcactttct atttctgcaa atgagatttt ccgtatggcg gctattgctt
     1141 ttatcttact tacggttttg gtttggtttg cgaaaccgcc gtttacagcg aaaggcgttg
     1201 ggtgatggaa aaagaggagg ctatagcagg taaaacggca gaatcttaag tctgcgtcat
     1261 cggcagcatc gctcacccag ggaaaggatg gcgatgctgc gttgaaacgt tattaacggc
     1321 cttttgccag atattgattc atctcttctt ccggcaccat tccacctccc gtcgcccaca
     1381 ccagatgagt ggcattacgc agttgttctg cgctgaaacc gtgcatctgt tggtaactta
     1441 ctgatgcgca cacgcgctga ggcccggcca tacccgccag tgccgatggt tcaagacgaa
     1501 taccttcttc ctgcgccagc cagctaagca tgtcatacat ggtttgatcg ctaagggtat
     1561 agaagccatc cagcagacgc tccattgccc gcccgacaaa gcccgatgcg cgaccaaccg
     1621 caaggccatc ggcagcggta aggttgtcga tgccaatatc ctgaacagaa atctgatcgt
     1681 gtaatccggt atggacgcct aataacatac acggggagtg cgttggttcg gcaaaaaagc
     1741 agtgaacatg atcgccaaac gccagtttaa gtccgaatgc gacgccacca ggaccaccgc
     1801 caacaccaca cggcagatag acaaacagag ggttatcagc attgacgatg cgaccttgct
     1861 gagcaaattg tgccttaaga cgctggcctg cgacggaata tccaaggaac aacgtgcggg
     1921 aattctcgtc atcaataaag aaacagttcg ggtcagactg cgctgcttta cgtccttcct
     1981 cgacagcaac accataatct tgctcgtatt ccacgacagt aacgccatga ctgcgcagtt
     2041 tcgctttttt ccaggcgcgg gcatcagcag acatatgcac cgttacttta aagccgatgc
     2101 gggcgctcat aataccaatt gataacccca gatttccggt tgatcccaca gcaatactgt
     2161 attggctaaa gaactgttta aactccgggg aaagcagttt gctgtaatca tcctcaagtg
     2221 tcagcaaacc cgcttccaga gccagttttt cagcatgggc caggacttca taaatcccgc
     2281 cacgtgcttt tatggagccg gaaatcggca aatggctgtc tttttttagt agcaattgcc
     2341 cggcgatcgg ctggtgatat tccttttcca gccgtttttg catagcagga atagcaacca
     2401 gttctgattc aataatcccc cccgcggcag ccgtttcagg aaatgctttt gccagatacg
     2461 gcgcaaaacg agacaggcgc gcatgggcgt cctgaacatc ctgttcggtc aggccaacat
     2521 aaggtaaacc ttctgccaat gaggtcgtgc caggattaaa ccaggtggtt tctttaagag
     2581 caaccagatc ctctaccaac ggatactggg cgatgagcga attcatttta gcgttttcca
     2641 tagtacgtct ctttgcgctt agatgataaa tgacagcagg aatgtgcccg ccagagcgat
     2701 gactgaagcg ataaatgtcg ctgtcgtata gtatttaaat gtttcattga gcgtagcgcc
     2761 gcaatattgc ttcaccagcc agaaaagcga gtccgtaacg atcgtgcagc caatcgcacc
     2821 tgaaccgatg gcaattgcaa taatttccgg gctgatgtcg ggatacagcg gcagcatggg
     2881 tgcaacaatt gccgttgccc ccatcattgc caccgtagcg gagcccactg cagcatgcag
     2941 aataagtgcc accaaccagg ccagaagaat cgggtgcata tgcatattgg agagaataac
     3001 tgccagcgta tcagcgagac tgctgctttt taaaatggcg ttgaatgcgc ctccggcccc
     3061 gataatcagc aaaatattag caatagaacc gaaaccattt tccgtatgtg tgagcatcgt
     3121 ccccatgctc atatgctgac gtatacccaa cacataatag gcgacaaaca cggcgataaa
     3181 catggcagtg atagggttgc caataaactc aagcaaggta tacaaaccac tctcacgtgc
     3241 catattcaat tcggcaatcg ttttaaccaa catcagcgca atgggtagca gtacggtgaa
     3301 taacgttgcg cctaatgacg gtagtgtttt ttcatcgcga actttgagat cggcaaactc
     3361 tgtgggtaca ggtttaaagg gcagtcgttg acccagaaat ttaaggaaaa gtgggccacc
     3421 gatcagtgat gccatcagcc caaccagcaa accgtagacg atcaccgaac cgatatctgc
     3481 gcccagctta ttggcaacat ataaagcagc cggatgtggg ggaaccacgc agtgcactgc
     3541 catcaatgcg gtacacagcg gaatggccag ctttaacaat gaggtattgg tttttttggc
     3601 aattgaaaaa gccagaggaa tcaatagcac gacgcccact tcaacaaaca gcgtgatgcc
     3661 gcaaatcagg ccaaccagca ccataatgac atcagctgaa agccaccggc agcgttgaag
     3721 tgtcagacca attctttctg cggccccgga tacttccatc atttttccca gtatcgtgcc
     3781 aaggccgata accgcagcga ggaaccccag cgttccgcca attccacttt caatagcatt
     3841 taccatatcc agtggcccca tgcccatcat cgttcccacg aagaagctgg ccagcaacag
     3901 cgccagaaac gggtggaatt tgaacttcac gatggtcagt acaattaaca cgatgctgat
     3961 aagcagcgtg ctcacaaccc agatttgaga gtgcatatct caccttcccc tgtgattaac
     4021 ctgatgatat tggacaaaaa aacaacgcag gctgacaaac gataaaattt gcttttcacg
     4081 tgagctaaat tgcatcaatg gagtgattta catctaaaaa tgaaggtgaa ttgagatatg
     4141 gttcacttta gctcaccttg cagctgaaat tttgccgcta tttttttaca cttaagagaa
     4201 aaatgaggtg attatggaac cccttcgtga aataagaaat cgactgctta acggctggca
     4261 actatcaaaa ctgcatactt ttgaagtggc tgccaggcat cagtccttcg ccctggcggc
     4321 agaggaattg tcgctgagcc ccagtgcggt aagtcaccgt atcaatcagc tggaagaaga
     4381 attaggtatt cagttgtttg ttcgttccca tcgcaaagtg gaattaacgc acgaggggaa
     4441 acgtgtttat tgggcgctaa aatcgtcgct ggataccctg aatcaggaaa ttctggatat
     4501 caaaaatcag gagttatcgg gaacgttaac tctgtattcc cggccctcta tcgcccaatg
     4561 ctggttggtg cccgcattag gtgactttac acgccgatat ccgtctattt cgctcaccgt
     4621 gctcactggt aatgacaatg tcaatttgca acgtgccgga atcgatttgg cgatctactt
     4681 tgatgatgcg ccgtcagcgc aattggctca tcactttctg atggatgaag aaatcttgcc
     4741 agtttgcagc ccggagtacg ctcaaagaca tgatttaacc aacacggtaa ttaacctgcg
     4801 tcactgtacg ttgctccatg acagacaggc atggagcaac gactccggta cggatgaatg
     4861 gcatagttgg gcgcaacatt atgcggttaa tttgccaaca tcttctggaa ttggctttga
     4921 tcgctctgat ttagccgtta tcgccgcgat gaatcatatt ggggtggcga tgggaaggaa
     4981 acgtctggta caaaaaaggc ttgccagtgg tgagctcgtc gcgccgtttg gcgatatgac
     5041 ggtgaaatgc catcagcatt attacatcac cacattaccg ggcaggcagt ggccaaaaat
     5101 tgaggcattt attacttggt taagagaaca ggtaaaaacc acttcataat gaataagacc
     5161 acgagttcct ggtgtgcctt attcggcact gctagtgcga atttaataat ttacggtttt
     5221 tacgtggttt tagggataaa cacaataatg gtgtccctgc aggaatcgaa gtaaatatta
     5281 actctatg
//
LOCUS       DADSZD010000233         5266 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.235, whole genome shotgun sequence.
ACCESSION   DADSZD010000233 DADSZD010000000
VERSION     DADSZD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.235"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            167..1012
                     /locus_tag="J1Z28_004158"
     CDS             167..1012
                     /locus_tag="J1Z28_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7718929.1"
                     /translation="MNNITVKSLIQSNYPTLHQAEKKVADYILSHAHEVVNYSVTELS
                     EKSHASEATIVRTCKKLGYQGYYHLKIALAKEVINPDNSYPDNTDFTDITSLATFLLK
                     KQAEDLIQSTQFFNAEVLESILKLLANCDTIFFFAAGNSNPLAVYGAYKFSQLGLKTV
                     VHVSPEMQINAAYSMGKRDLAIGISNSGSTNLIVDIFKVVKERGAKSVCITNYIKSPL
                     SKLSTHQLNTAVSDKIFFEAFDSTRVPAMGVIDMLVLLFMYKNKAHYEKYRTREEFLG
                     SRFKG"
     gene            complement(1042..3039)
                     /gene="tkt"
                     /locus_tag="J1Z28_004159"
     CDS             complement(1042..3039)
                     /gene="tkt"
                     /locus_tag="J1Z28_004159"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005971397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBA7718930.1"
                     /translation="MNSQLLANAIRMLSVDAIQKANSGHPGAPMGMADIAEVVWRRHL
                     RHNPKNPQWFNRDRYVQSNGHGSMLIYALLHLTGYDLSMDDIRDFRQLHSRTPGHPEY
                     GYTPGVETTTGPLGQGVANAVGMAIAEKALAAEFNKPGFNIVDHHTWLFLGDGCLMEG
                     ISHEACGLAGTLKLGNLIAIWDDNGISIDGHVEGWFAEDTAARFRAYGWHVIEGVDGH
                     DPEAVDAAVREAKSVTDKPSLLCCKTIIGFGSPNKANSHDCHGSALGADEVALVRERL
                     QWPYAPFEIPGEIYAEWDATEKGAQVQQEWDALFADYAKQWPELAAEFTRRMKGDLPA
                     GWVENMQKYVHDLQSHPAALATRQVSQKCLNHFADMLPELMGGSADLSPSNLTRHQKS
                     VDFTGENPAGNYISYGVREFGMSAIMNGLALHGGFIPYGGTFLMFMEYARNALRMAAL
                     MKIRSVFVYTHDTIGLGEDGPTHQPVEQLASLRLTPNMETWRGCDQVEVAVAWQQAIE
                     RKDGPTSLVLTRQPLAQQPRTAAQLAEIARGGYVLSDCDGQPEMILISAGSEIELVVS
                     AAKALTEEGRKVRVVSMPCTERFDNQDAAYKESVLPKAVRKRLAVEASIAGFWERYVG
                     LDGKVIGMTSFGESAPANVLFKHFGFTPENVLAQARELLNS"
     gene            complement(3090..4598)
                     /locus_tag="J1Z28_004160"
     CDS             complement(3090..4598)
                     /locus_tag="J1Z28_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA7718931.1"
                     /translation="MSKKITSLFNFKKLQKFSSALMLPIAILPAAGLILAIGISFNIE
                     LLANCGGLIFANLPLLFCVGIVIGLTGDGSSSLSAIISYMIMNATMGTQLGITAESVA
                     ASNGDYAMILGVPTLQTGVFGGLIVAIFVYLLYTRFHNIELPQFLGFFGGKRFVPIIT
                     AVAAIIVGYLLPWLWIPVQKGLASLSTLVTSGHNANFAAFIYAVGERSLIPFGLHHIW
                     NVPFYYNFGDYMTKSGQLVTGDIPVFFAQLRDGVPLTAGLFMTGRFPIMMFALPAAAL
                     AMYQEAKPERKALIKGLLLSGALTTFVTGITEPLEYAFLFAAPVLYIIHIFLYATSFV
                     LMNILNVHIGHPFAGGLIDFVLNGVMPNRTPWYLVFLAGAGYAAIYYTVFRVLIRKLN
                     LKTPGREDEEIAVATTLTREERPYQIIEAVGGFDNIEDVDACATRLRLALVDDKKVNE
                     KRLKELGAAGLVKLGDGGVQVIFGGKSQILRDEIKTVMSRPRPTEMTVACAN"
     gene            complement(4640..5197)
                     /locus_tag="J1Z28_004161"
     CDS             complement(4640..5197)
                     /locus_tag="J1Z28_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA7718932.1"
                     /translation="MDAKLYITEIINELTFVKDSIREDDCTKLIDAIQQSQRVFCYGL
                     GRAGFSMKAFTMRLMHMGKEVYFLTETITPNFGPGDLFIVSSASGETAQLVALAKKAR
                     QLGGAVAVLTTNRHATITEFVDVIVQINAPSKNQKDSVFRSAQPMASLYEQALLVIAD
                     ALVMKMAAESGAPESELFKRHANLE"
BASE COUNT         1396 a         1431 c         1180 g         1259 t
ORIGIN      
        1 attagagcgc ccgtttaaat tatttttgtg cagttgacgt aattcatgaa aattaaccgg
       61 tctgttaatc attacgttca ctgcacacct tcattttttc acacctatcc acgcctgaaa
      121 actatgttat aatcagcact ttctgtcaga atgaaattta ctaattatga ataacataac
      181 cgtaaaaagt ctgattcaaa gcaattaccc tacactgcat caggcggaaa aaaaggtcgc
      241 tgactatatt cttagccacg cacatgaagt cgtgaactat agtgtcactg aactatcaga
      301 aaaaagccat gccagtgaag cgactatcgt acgcacctgt aagaaattag gttatcaggg
      361 ttattaccat ttaaaaattg cattagcaaa agaagtcatt aatcctgaca atagctatcc
      421 tgataatacc gattttaccg atataacgtc gctggccaca tttttattaa aaaaacaggc
      481 cgaagattta atacaatcaa cgcaattttt taatgccgaa gtgcttgagt ccattctcaa
      541 actacttgct aattgcgaca ctattttctt ttttgctgcc ggaaattcaa accctctggc
      601 cgtttatggt gcttataaat tcagccaact cggattaaaa accgttgttc acgtcagtcc
      661 ggaaatgcaa attaatgcgg catattctat ggggaaacgt gacctggcga ttggtatttc
      721 taactctggc agcaccaact taatagtcga tatctttaaa gtggtgaaag agcgcggtgc
      781 caaatccgtt tgtatcacta actatattaa atctccgtta tcaaaactct ccacccatca
      841 actcaatacc gcggtcagcg ataaaatctt ctttgaagcc ttcgactcca ctcgcgttcc
      901 ggcaatgggc gttattgata tgctggtgct gttgtttatg tataaaaaca aggcgcatta
      961 cgagaaatac cgcacccgtg aagagtttct tggctcccgt ttcaaggggt aaaaggtcat
     1021 cctttcaccc cttgtgcagg tttagctgtt cagtagctca cgcgcctgcg ccaggacatt
     1081 ttccggcgta aaaccaaagt gtttgaacaa cacattcgcc ggagcagact caccaaagct
     1141 cgtcatgccg atcactttac cgtccaggcc gacgtagcgt tcccagaatc cggcgatgct
     1201 ggcttctacc gccagacgtt tgcgtacagc ttttggcaat accgactctt tgtacgccgc
     1261 atcctgatta tcgaaacgct cggtacacgg catggaaacc acgcgaactt tacgcccttc
     1321 ttccgttaac gcttttgcgg cgctgaccac taactcaatc tctgaacccg cagaaatgag
     1381 gatcatctca ggctgaccgt cacaatcact cagtacataa ccaccacgcg caatctctgc
     1441 caactgtgca gcggtgcgcg gctgttgcgc cagaggttgg cgcgttaata ccaacgacgt
     1501 tgggccatct ttgcgctcaa tcgcctgttg ccaggctacc gccacttcca cctgatcgca
     1561 accgcgccag gtttccatat tcggcgtcag acgcagcgac gccagttgct caacgggctg
     1621 atgtgtcggg ccgtcttcgc ccaggccgat ggtatcgtgg gtataaacaa acaccgagcg
     1681 gatcttcatc agcgccgcca tgcgcagtgc attacgcgca tattccataa acatcaggaa
     1741 agtaccgccg taagggataa acccaccatg cagcgcaaga ccgttcatga tggccgacat
     1801 accaaattca cgcacgccat aggagatgta attccccgcc ggattttccc cggtgaaatc
     1861 aaccgatttc tgatggcgag tcaggttaga gggcgacaag tccgccgagc cgcccatcag
     1921 ttccggcagc atatccgcga agtgattgag gcatttctgg ctgacctgac gggttgccag
     1981 cgccgccgga tgggattgca gatcgtgcac atatttctgc atattctcga cccagccagc
     2041 aggcaaatca cccttcatgc gacgagtaaa ctccgccgcc agttccggcc actgcttcgc
     2101 gtagtcggca aacagtgcat cccattcctg ttgaacctgc gcgcctttct cggtcgcatc
     2161 ccattcggca taaatttcac caggaatttc gaaaggggca tacggccatt gcagacgttc
     2221 acgcaccagc gccacttcat ctgcacccag cgccgagccg tggcaatcgt ggctgttggc
     2281 tttatttggc gaaccaaaac cgataatcgt tttgcagcac agcagactgg gcttatccgt
     2341 gacgcttttc gcttcacgca ccgcagcatc taccgcttct ggatcgtgac catcaacgcc
     2401 ttcaatgaca tgccagccgt aggcacgaaa acgggcagcc gtatcttcag caaaccagcc
     2461 ttctacgtga ccatcgatcg aaataccgtt atcgtcccag atagcaatca gattgccgag
     2521 cttcagcgtt cctgcaagac cacaggcctc gtgggagatc ccttccatca ggcagccatc
     2581 acccaaaaat agccatgtgt gatgatcaac aatattaaag cccggtttgt taaattctgc
     2641 cgccagcgcc ttctccgcaa tcgccatccc caccgcatta gctacgccct gccccaacgg
     2701 gccggtggtg gtttcaacac ccggcgtata accatattct ggatgacccg gcgtgcggga
     2761 gtggagctga cgaaagtcgc gaatatcatc catcgacagg tcatagcctg taagatgcag
     2821 caaagcgtaa atcaacatgg agccgtgacc gttcgactga acatagcggt cgcggttaaa
     2881 ccactgcgga ttttttgggt tatgacgcag gtgacgacgc cagaccactt cggcaatatc
     2941 cgccatcccc atcggcgcgc ccggatgacc ggagttcgct ttctggatcg catcaacact
     3001 taacatccgg atggcgttag ccagtaattg tgaattcata cttcagctct ctctgtctgg
     3061 cgggctgaaa ccagcccgct aatctcgaat tagttagcac aggccactgt catttctgtc
     3121 gggcgcggtc tgctcattac ggttttgatt tcgtcgcgca ggatttgcga tttgccgcca
     3181 aatatcacct gtacaccgcc atcacccagt ttcactaaac ccgcagcccc caactccttc
     3241 agccgttttt cattcacttt cttatcgtct accagcgcca gccgcaggcg tgttgcgcag
     3301 gcatccacat cttcgatatt gtcgaaacca cctactgctt cgatgatttg gtatggacgt
     3361 tcttcgcgcg ttaatgtggt tgcaacggca atctcttcat cctcacgccc cggtgttttc
     3421 agattcaact tgcggatcag tacccgaaat acggtgtagt aaatcgctgc atagcccgcg
     3481 cctgccagga acaccagata ccacggcgtg cggtttggca taacgccatt cagtacgaaa
     3541 tcaataagac cgccagcaaa aggatgacca atgtgcacat tcaggatgtt catcagcaca
     3601 aatgaagtgg catataagaa gatgtgaatg atgtagagaa ccggagctgc aaacaaaaat
     3661 gcatattcca gcggctccgt aatcccggta acaaatgtgg tcagcgcacc ggagagtaaa
     3721 aggcctttaa tcagcgcctt acgttccggt tttgcttcct gatacatcgc caacgcggcg
     3781 gcaggtaacg caaacatcat gattgggaaa cgacctgtca taaacagccc cgccgtcaac
     3841 ggaacaccgt cgcgcaattg ggcaaagaac accggaatat cgcccgtcac cagttgcccg
     3901 gatttggtca tgtagtcgcc aaagttgtaa taaaacggca cgttccagat atgatgcagg
     3961 ccaaaaggaa tcagcgaacg ttcgccaacg gcgtaaataa aggccgcgaa gttcgcgtta
     4021 tgcccactgg tcaccagcgt tgaaagcgac gccagacctt tttgtaccgg tatccacagc
     4081 cacggcaata aatagccgac aataattgcc gccaccgccg taataatcgg tacaaagcgt
     4141 tttccaccga agaaaccaag aaactgaggc aattcaatat tgtggaagcg ggtatagagc
     4201 agataaacaa agatggcgac aatcaaaccg ccaaacactc ccgtttgtaa cgtcggtacc
     4261 ccgaggatca tcgcatagtc accgttactg gctgcaacgg actccgcggt aatgccaagt
     4321 tgcgtaccca tcgtggcatt catgatcatg tagctgataa tcgctgaaag tgaagaagaa
     4381 ccatcacctg tcaggccaat cacaataccg acacaaaaca gcaaaggaag gttggcgaaa
     4441 atcaggccgc cgcaattcgc cagcagctca atgttaaatg aaatcccgat ggctaaaatc
     4501 aaacccgccg ccggaagaat ggcgattggt aacatcagag cagaagaaaa tttctgcaac
     4561 ttcttgaagt taaacaagga tgttattttt ttgctcattt ttattctcct gtagggtatc
     4621 gagcaaataa cggcgacgac tattccagat tggcgtggcg cttaaacagt tcactttccg
     4681 gcgcaccact ttcagcagcc atcttcatca ctaatgcatc ggcaatcacc agcaaagctt
     4741 gctcatacaa cgacgccatc ggctgtgccg agcggaaaac agaatctttt tgatttttgg
     4801 aaggggcatt gatttgcacg atgacgtcga caaactcggt gatagtggcg tgtcgattcg
     4861 tggttaacac cgccacagcg ccgccaagct gacgtgcttt tttcgccagt gccaccagtt
     4921 gtgctgtctc accggaagcg gaagaaacga taaacagatc gccaggccca aagttcggcg
     4981 taatggtttc cgtcaggaag taaacctctt tgcccatatg catcaggcgc atggtgaagg
     5041 ctttcatgga aaagccggca cgccccaggc catagcagaa aacgcgctgg ctttgctgaa
     5101 tagcgtcgat caatttggtg cagtcatcct cacggataga gtccttaacg aaggttaatt
     5161 cattgatgat ctcagtgatg tacagctttg cgtccatttc acacctcatg aaataatttt
     5221 tctcaaacac aaaatctcat gaaattttat ttcattccaa tgcatc
//
LOCUS       DADSZD010000234         5122 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.236, whole genome shotgun sequence.
ACCESSION   DADSZD010000234 DADSZD010000000
VERSION     DADSZD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.236"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            8..1804
                     /locus_tag="J1Z28_004162"
     CDS             8..1804
                     /locus_tag="J1Z28_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002954510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HBA7718933.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKVMKDDYVVHTSAIVNNYMDNRSISDLQQTKVIKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDT
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            1822..2490
                     /locus_tag="J1Z28_004163"
     CDS             1822..2490
                     /locus_tag="J1Z28_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HBA7718934.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQSKKAKFAYYAAYHDKKERWSDGKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2505..3176
                     /gene="dsbI"
                     /locus_tag="J1Z28_004164"
     CDS             2505..3176
                     /gene="dsbI"
                     /locus_tag="J1Z28_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
                     /protein_id="HBA7718935.1"
                     /translation="MGIKGMWKDLRTSPVDTLVRWQEQRLLWLLMAVAMGALIILAHS
                     FFQIYLYMAPCEQCVYIRYAMFVMVIGGLVAAINPKNIILKLIGCVMAFYGSILGLKF
                     SLKLNDIHHAVHNPDPDSLFGVQGCSTDPTFPFNLPLAQWAPNWFKPTGDCGYDAPIV
                     PDGVTLSSTQQWFVEMYQQSEGWYLLPPWHFMNMAQACMLAFGMCLVLLVIMSGAWAH
                     KIIRG"
     gene            complement(3222..4037)
                     /gene="ygiD"
                     /locus_tag="J1Z28_004165"
     CDS             complement(3222..4037)
                     /gene="ygiD"
                     /locus_tag="J1Z28_004165"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311954.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBA7718936.1"
                     /translation="MTPLVKDIIMSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMT
                     LPRPQAIVVVSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTYYPAPGSPALAQHLV
                     ELLAPVPVTLDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLA
                     ALRDEGIMLVASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVN
                     YLDHEGGALSNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4153..4926
                     /gene="zupT"
                     /locus_tag="J1Z28_004166"
     CDS             4153..4926
                     /gene="zupT"
                     /locus_tag="J1Z28_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBA7718937.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
BASE COUNT         1321 a         1259 c         1264 g         1278 t
ORIGIN      
        1 tatatccatg tttgataaat atagaaaaac actcgtagcc ggaactgtgg cgataaccct
       61 gggtttgtca gcatcggggg tgatggctgc gggttttaaa ccagcgccac ctgccgggca
      121 actgggtgcg gtcattgtcg atccctacgg caatgcacca ctgaccgctt tggttgactt
      181 agatagccat gttatttctg acgtcaaagt taccgtccat gggaagggcg aaaaaggcgt
      241 agaaatcagc tatcccgtgg gtcaggaatc actaaaaact tacgatggtg taccgatttt
      301 tggtctttat cagaaatttg ctaacaaagt gaccgttgag tggaaagaaa acggcaaggt
      361 catgaaagat gattatgtgg tgcacacttc ggccatcgtc aataattaca tggataaccg
      421 ctctatctcc gatttacaac agaccaaagt tattaaagtc gcaccgggtt ttgaagatcg
      481 cctctatctg gttaataccc acacctttac cgcccaaggt tccgatctcc actggcatgg
      541 tgagaaagat aaaaatgccg gtatccttga tgcgggtccg gcaactggcg cactcccttt
      601 tgatatcgcg ccattcacct ttatcgtcga taccgaaggt gaataccgct ggtggctgga
      661 tcaagacact ttctacgatg gtcgtgaccg cgatatcaac aagcgtggtt atctgatggg
      721 tatccgcgaa acgccacgcg gcacctttac cgctgtacaa ggtcagcact ggtacgagtt
      781 cgacatgatg gggcaggtgc tcgaagatca caaactaccg cgcggatttg ctgacgccac
      841 tcatgagtcc attgagacgc caaatggcac ggtactgctg cgcgtaggta agagtaacta
      901 tcgtcgcgat gacggcgtac acgtcaccac cattcgtgac catatcctcg aagtcgataa
      961 atctggtcgc gttgtcgatg tatgggatct gaccaagatc ctcgatacga aacgcgatgc
     1021 actgctcggc gcgctggatg caggtgcagt ttgcgttaac gttgaccttg cccatgcagg
     1081 acaacaggca aaactggaac cagatacacc gttcggcgac gcgctgggtg tagggccagg
     1141 ccgcaactgg gcgcacgtga actctatcgc ctatgacgca aaagatgact ctatcatcct
     1201 ctcctctcgc caccagggtg ttgtgaaaat tggtcgtgat aagcaagtga aatggatcct
     1261 tgcgccctct aaaggttggg aaaaaccgct ggccagcaag ctgctgaaac cggttgatgc
     1321 taacggtaag ccaattacct gtaacgaaaa tggcctgtgc gaaaactcag acttcgactt
     1381 tacctacacc cagcataccg cctggatttc cagcaaagga acgctcacca tttttgataa
     1441 tggcgatggg cgtcatctgg aacaacctgc cttaccaacc atgaaatact cccgctttgt
     1501 ggaatataag attgatgaga agaaaggcac cgttcagcaa gtgtgggaat acggtaaaga
     1561 acgtggctac gatttctata gcccgattac ctccatcatt gaatatcaag ccgaccgtaa
     1621 caccatgttt ggcttcggtg gttctattca tttgttcgat gtcgggcagc caaccgtcgg
     1681 taagttgaac gaaatcgatt acaaaaccaa agaagtgaaa gtggaaatcg acgtgctgtc
     1741 agataaaccc aatcagactc actaccgtgc gctgttagtc cgtccacaac agatgttcaa
     1801 ataattaaga agggaataaa aatgtcgaaa ttagggatct catcactatt taaaaccata
     1861 ctgcttaccg cagctctggc ggtttcattt accgcttctg catttaccga aggcacggat
     1921 tacatggttc tggaaaaacc aattccgaat gccgacaaaa cgctgattaa ggtatttagc
     1981 tacgcctgcc cgttctgtta caagtacgac aaagcggtta ccggtccggt atcggaaaaa
     2041 gtgaaagata tcgttgcttt cacgccattc catctggaaa caaaaggtga atacggcaaa
     2101 caagcaagcg aagtctttgc ggtcttgatc aataaagata aagcagcagg catttcctta
     2161 tttgatgcta actctcagtc taagaaagcc aagtttgcct actacgcggc ttatcacgac
     2221 aaaaaagaac gctggtctga cggtaaagat ccggctgctt ttatcaaaac cggtctggat
     2281 gccgcaggta tgagtcaggc ggattttgaa gcggcactga aagaaccagc agttcaagaa
     2341 acactggaaa aatggaaagc ctcttatgat gtcgccaaaa tccagggggt tccagcctat
     2401 gtcgttaatg gtaagtactt gatctacacc aagagcatca aatccatcga cgcgatggca
     2461 gaccttatcc gtgagttagc aagcaaataa cagggaaaca ggctatgggt attaagggaa
     2521 tgtggaaaga tcttcgcaca tcaccagttg acacactggt cagatggcag gagcaacgct
     2581 tgttgtggtt gttaatggcc gttgcgatgg gcgcgttaat tatcctggcg cactctttct
     2641 tccagatcta tctctacatg gcaccctgtg agcaatgtgt atacattcgc tacgccatgt
     2701 ttgtgatggt tattggagga ttggttgcag cgatcaaccc aaagaacatc atcctaaagc
     2761 taattggctg cgtgatggca ttttacggca gcattttggg actgaagttt tcgctcaaac
     2821 tgaacgatat ccaccatgca gtacataatc cggatcccga ttcactgttt ggcgtacagg
     2881 gttgctctac cgatcccact tttcccttta acctgccgct ggcacagtgg gcaccaaatt
     2941 ggttcaagcc taccggggat tgtggctatg acgcaccaat cgttcctgat ggcgtcacac
     3001 tgagcagcac gcaacagtgg tttgtggaaa tgtatcagca atctgaaggc tggtatctgc
     3061 taccaccatg gcattttatg aatatggctc aggcttgtat gctggctttt ggtatgtgtc
     3121 tggtgctgct ggtcattatg agtggcgcat gggcgcataa aatcatcaga ggctaactca
     3181 gcaacacccg aagccagtca ttctggcttc gggttctttt attaacctat ctgcaccgac
     3241 agcatactta aactgcccat ttctatacca tcgaccggaa tggtaattgg ctcctgccca
     3301 tcccacgccc ctaagacata caacaacggc agatagtgct ctggcgttgg gttcgataac
     3361 gcgccacctt catggtcgag ataattcacc agaggatgtt gttccaccgg cccttgccac
     3421 gtcagattcg ctttcacata ctcattaaac gacgtcgccc acggatacgg tgaactatca
     3481 ccgtgccact tcactgtacg caggttatgt accacgttgc cactggcgac caacattatc
     3541 ccttcatcac gcagcgccgc gagtttgcgc cccatttcga aatgccaggc ggcaggtttg
     3601 ctactgtcga tactcaactg caccatcggg atatcggcgt cagggtacat cttaatcagt
     3661 acgccccacg agccgtggtc gaagccccag gcttctttat ccagcgtcac agggaccggc
     3721 gctaacagct caaccagatg ctgtgccagc gcaggtgaac ccggagcagg ataatacgta
     3781 tcgtacagcg cctgcgggaa gccaccaaag tcatgaatcg tgggcggcat ctccatcgcg
     3841 gtcactcctg ttccacgggt aaaccaatga gccgaaacca ccactattgc ttgcgggcgt
     3901 ggcaatgtca tccccaacgt ctgccagctg cgggtataca aattatcttc cagcacgttc
     3961 atcggactac cgtgacctaa aaacaatgct ggcatacgtg ttgaagacat gatgatatcc
     4021 ttaactaaag gtgtcatttt gatatcctca caatacgctt gttcggcgga gtaagaaccc
     4081 ggataacaat gatgatgatc atcagttatt ttgacgatct gcctgaaggt aaagatttat
     4141 aaggagttgt cgatgtcagt acctctcatt ctgaccatac tggcgggggc agccacgttt
     4201 attggcgcgt ttctcggcgt tctcgggcaa aaaccctcga accgcttact ggcgttttcg
     4261 ctaggatttg cggcggggat catgttgctc atctcattaa tggaaatgct tcctgccgca
     4321 ttagccgccg aaggaatgtc gcctgtgttg ggttatggaa tgtttatctt cggtctgctt
     4381 ggctattttg gcctggaccg catgttgcca catgcgcatc cgcaggattt aatgcaaaaa
     4441 tcggtgcagc cgttgccaaa atcgatcaag cgcacagcca ttctgctcac tctcggcatc
     4501 agtctgcata acttcccgga agggattgcc acctttgtca cggcgagcag caacctggag
     4561 ctgggatttg gcatcgccct ggccgtagcg ttgcacaata ttcctgaagg tctggcggtt
     4621 gcaggcccgg tttacgctgc aacaggatcg aaacgtaccg cgattctgtg ggcgggcatt
     4681 tccgggctgg cagaaattct tggtggtgtg ctggcatggt taatcctcgg tagcatgatt
     4741 tctccggtgg tcatggcggc aatcatggcg gcggttgcag gaattatggt ggcgctctcc
     4801 gttgacgaat taatgccgct cgccaaagag attgacccca ataataaccc cagctatggc
     4861 gtactatgtg gaatgtcagt gatgggattc agtttagtgc tgctacaaac ggtgggaatt
     4921 ggttaaagac gataatgaca gatgttgccc attgctaaag gcaacatctg tatcgttatt
     4981 aaaggtatta ttgatgcttt tcaaacactt ctttatcgga gcgtttatct aatttacttc
     5041 gggcagctaa cagcgcatct ctgtcatggc gaaaagcttt acagtaacgt ttaagatgaa
     5101 gaaaatagcc accagccagt ag
//
LOCUS       DADSZD010000235         5114 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.237, whole genome shotgun sequence.
ACCESSION   DADSZD010000235 DADSZD010000000
VERSION     DADSZD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.237"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..228
                     /locus_tag="J1Z28_004167"
     CDS             <1..228
                     /locus_tag="J1Z28_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HBA7718938.1"
                     /translation="KVKNINLDAIEDQKLGLVFNVIVSVEENDLSTGNKHIPLSSGMA
                     VTAEIKTGMRSVISYLLSPLEESVTESLHER"
     gene            complement(600..908)
                     /locus_tag="J1Z28_004168"
                     /pseudo
     CDS             complement(600..908)
                     /locus_tag="J1Z28_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(925..2001)
                     /locus_tag="J1Z28_004169"
     CDS             complement(925..2001)
                     /locus_tag="J1Z28_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc21 family transposase"
                     /protein_id="HBA7718939.1"
                     /translation="MAGKVTETAVVGGVDTHKDLHVAAVVDQNNKVLGTQFFSTTRQG
                     YRQMLAWMTSFGALKRIGVECTGTYGSGLLRYLQNAGLDVLEVTAPDRMERRKRGKSD
                     TIDAECAAHAAFSGIRTVTPKTRNGMIESLRVLKTCRKTAISARRVALQIIHSNIISA
                     PDELREQLRNMTRMQLIRTLGSWRPDASEYRNVTNVYRISLKSLARRYLELHDEIADL
                     DVMIAAIVDELAPELIKRNAIGYESASQLLITAGDNPQRLRSESGFAALCGVSPVPVS
                     SGKTNRYRLNRGGDRAANSALHIIAIGRLRTDDKTKEYVARRVAEGHTKMEAIRCLKR
                     YISREVYTLLRNQNRQINSIPITA"
     gene            complement(2283..3139)
                     /locus_tag="J1Z28_004170"
                     /pseudo
     CDS             complement(2283..3139)
                     /locus_tag="J1Z28_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3431..3604)
                     /locus_tag="J1Z28_004171"
                     /pseudo
     CDS             complement(3431..3604)
                     /locus_tag="J1Z28_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3723..4947
                     /locus_tag="J1Z28_004172"
                     /pseudo
     CDS             3723..4947
                     /locus_tag="J1Z28_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1242 a         1257 c         1342 g         1273 t
ORIGIN      
        1 aaggtgaaaa atataaattt agatgcaata gaagaccaga aactgggact cgtttttaat
       61 gtcattgttt ctgttgaaga gaatgatttg tcaaccggga ataagcacat tccattaagc
      121 tcgggtatgg ctgtcactgc agaaataaag actggaatgc gaagcgtaat cagctatctt
      181 cttagtcctc tggaagagtc tgtaacagaa agtttacatg agcgttaagt ctcagagccg
      241 cggtatccgg ctcatatctt ctcctgtcat gtgtgactgc actgttttgt ggcaagccag
      301 tactgactat ccactggaat tcggtagtca ttaactatgg cattgatgtc ctttataact
      361 gtcttacagg gcgtataaca catcccgcga cggaagctta tcacagagga agaggtaagc
      421 ttcagaatgg aaagtatatg agaagactgt gattgtttaa aaatacacaa tccgctgtga
      481 ggtggagtca ggggaggtac tggcccgcag aagcggagaa tggttgatgt atgaggcgag
      541 tgtgacattt ttcatgaaag taaaacaggt gataacctcc tttggttagg gtgtagtggt
      601 caactaattt tggccacacg acctgactgt tcgtggaaca gctgctctga ttcatttggc
      661 gttaagccac cgttatacca gtggggccgg atggcactgt aatagcctgt gatgtagcgg
      721 attatcgcgc tctgagcctc gttgaagctg ttatagccct tcgtcggcac ccattcggtc
      781 ttcaggctcc ggaagaaccg ctccatcggg gcattatccc agcagttacc ccgtcgactc
      841 atactctgct ttatccgaca gcgccacaga gcctgccggt actggcggct ggtatagtgg
      901 ctgccctgga cgcccttcta agagtcaagc cgttatcggg atgctgttga tctgcctgtt
      961 ttgattacgc agtaatgtat aaacttcgcg tgagatatag cgcttcaggc agcgtattgc
     1021 ttccattttt gtatgccctt ccgctactcg tctggcgaca tattccttcg ttttatcgtc
     1081 agttcgcaaa cgtccgatgg caatgatgtg aagtgcacta tttgcagcac gatctccacc
     1141 ccggttaagt cgataacgat tcgtttttcc tgaagatacg ggaacagggc tgacaccaca
     1201 cagtgccgca aaacctgatt ctgatcttaa tcgttgggga ttgtctcctg ccgtgatcag
     1261 caactgcgaa gcgctttcgt atccaatagc attacgttta atcagttcag gcgccagctc
     1321 atcgacaatt gccgcaatca tgacatccaa atcagcgatt tcgtcatgta actcgagata
     1381 gcgtcgggca agggacttta atgaaatacg ataaacgttg gtaacattgc ggtattcact
     1441 ggcatcaggc cgccaggatc ccagagtcct gatgagctgc atgcgcgtca tatttctgag
     1501 ctgttcacgt aattcatccg gggcagagat aatattggaa tggataatct ggagagcgac
     1561 tctgcgggct gatattgctg tttttcggca agtttttaat acccgcagag actcaatcat
     1621 gccattgcgc gttttggggg tgacggttct tattccggag aatgcggcgt gagcggcaca
     1681 ttcagcatca atcgtgtcac ttttaccccg tttgcgtcgc tccatccgat ctggcgcagt
     1741 cacctcaaga acgtctaacc cggcattctg taaatagcga agcagacctg atccataggt
     1801 gccagtacac tcaacaccaa ttcgctttaa tgccccaaac gaagtcatcc atgccagcat
     1861 ctgccggtaa ccttgccgtg ttgtggagaa aaactgggtc cccagaactt tattgttctg
     1921 atctacgaca gcggcaacgt gcagatcttt atgtgtatcc acgccaccca caacagcggt
     1981 ttccgtaact ttacctgcca taacagagct cctgtgagga aacagagttg aatctccaga
     2041 cagataaccc ggacaggaca gtaacgagac aaaccgtcag gcccttcttg agtcacgcgc
     2101 atcggtgagg agacgcctcg catgaaggcg cttccggcaa ccgacaggtc cagggcagga
     2161 cacaaagggt cgatcgctgt gtgagtcatg atgcgggaag gtcttcactg catcagtaat
     2221 cacaccagtc tggttaaaca gcaaacagag tgatgataaa aactgatacc ggcattatta
     2281 ctgtcactgt ggaacatcac gcctgttggc ttaccccgaa gctcccaggc catctgcaac
     2341 gctttgaccg tgagggcaga gtccggcgac gtcgacatcg cccagcccac aggtttgcgg
     2401 gcgaacagat ccagcactac tgccagataa gcccagcatt ttcccgtcca gatataggtc
     2461 acatcgccgc accagacctg atccggcgcg gtaaccgcga actgccggtc gagatggttc
     2521 ggtatttcaa cgtgttcgtt cccgccgcgt ttgtatttat gcaccggtac ctggcaactg
     2581 gagatatcca gctctttcat cagcttaccg gccagccatc gcccgagtct gacgccctta
     2641 gcgctgacca tcgtggcgat acttctcgcg ccagcagagc caccgctggc gttccagact
     2701 tcactgacga gactccgttt gacggcacgc tcagcatgag gccccctgct gtttttacga
     2761 atgtagcgat aactgcttcg gtgaacaccg aacagccggc aaaatggcgc taccgggtag
     2821 tgcgccctaa gactgtctat tatcgtgaac tgttcaggga gtccgacatc aagagcgcgg
     2881 tagccttttt taggatttca ttctccattt caaggcgttg tattcgtttt ctcatttccc
     2941 tgagttcagt ctgctcaggt gtcagaggca gccccggggg cgttttcccc tggcgctcca
     3001 tacgtaacga ttttacccag cggttgatgg cggagaggct gacgttcatc gccttagccg
     3061 cttcgccgtg agtgtagttc tgatccagga ccagttttgc cgcttcgact ttaaattcag
     3121 cagtaaatgc tttgctcatt ggttcacctg taagatgttg aggtgagcat atcacctcta
     3181 ctcaggttgc caaattcagt gtgccactac atatgtagtc aggcgtgata aatttcatat
     3241 ggaacaggta tgcgttttat ttgtgataac agttaatgag gtgtttccat acaccctgaa
     3301 gttacctgta atattagcgg gggatttgaa tgatgttgcg tggtctgcga ccactcgttt
     3361 attcatgcaa ataagtggac tgctggatcc acggtaagag tacagcgagg gccgtattga
     3421 cggggatgtg ttattcagcg ggcagtacta tgcgccacgg aagcagttcg ctgacacggt
     3481 tgaccggcca gtcagctatg acgccagaca catggcgaag gtagttttct ggatcctcgt
     3541 cgttcagttt gcacgtcccg atcaggctgt acagtagcac tccccgctca ccaccatgct
     3601 cagagctgcg tattaccgtg aaggagatcg gtagactggc cccctgaatc tccagacaac
     3661 cagtatcact taaataagtg atagtcttaa tactagtttt tagactagtc attggagaac
     3721 agatgattga tgtcttaggg ccggagaaac gcagacggcg taccacacag gaaaagatcg
     3781 caattgttca gcagagcttt gaaccgggga tgacggtctc cctcgttgcc cggcaacatg
     3841 gtgtagcagc cagccagtta tttctctggc gtaagcaata ccaggaagga agtcttactg
     3901 ctgtcgccgc cggagaacag gttgttcctg cctctgaact tgctgccgcc atgaagcaga
     3961 ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga aaatgaactc ctcaaagaag
     4021 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
     4081 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
     4141 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
     4201 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
     4261 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
     4321 ttaccggatc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
     4381 gaaacgggca catacaggca gagtggctgt gaaagaaagt aatcagcgat ggtgctctga
     4441 cgggttcgag ttccgctgtg ataacggaga aaaactgcga gtcacgttcg cgctggactg
     4501 ctgtgaccgt gaggcactgc actgggcggt cactacgggc ggcttcaaca gtgaaacagt
     4561 acaggacgtc atgctgggag cggtggaacg ccgcttcacg agcttccggc gtctccagta
     4621 gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacggca gtttgcccgg
     4681 atgttggggc ttgaaccgaa gagcacggcg gtgcggagtc cggagagtaa cggcatagca
     4741 gagagcttcg tgaaaacgat aaagcgtgac tacatcagtg tcatgcccaa accagacggg
     4801 ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg gcatccgcat
     4861 agtgcactgg gttatcgctc gccacgggaa tatctacggc agcaagccag taatgggtta
     4921 agtgataaca ggtgtctgga aatatagggg caaatccaat cggtgagtaa ccctctgtgt
     4981 cggcacatta tagccgtcac atcggataac tgttatcctt ctgttctgat atattctggg
     5041 aggtgatgtt tcactcctga taagagcatt actaattaca gctgcttttc ggataacatt
     5101 cgggcagttt tctt
//
LOCUS       DADSZD010000236         5072 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.238, whole genome shotgun sequence.
ACCESSION   DADSZD010000236 DADSZD010000000
VERSION     DADSZD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.238"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1024
                     /locus_tag="J1Z28_004173"
     CDS             <1..1024
                     /locus_tag="J1Z28_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA7718940.1"
                     /translation="CEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLE
                     THVFPFIGNRNITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGIIRY
                     NPALDMAGALTSVKRQHRPALDLSRLPELLSRINSYKGQPVTRLAVMLNLLVFIRSSE
                     LRYARWAEIDIDNSMWTIPAERKPLPGVKFSHRGSKMRTPHLVPLSKQAVAILTELQT
                     WAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTKEVCGHGFRAMACSALIESGLW
                     SRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREECISPFEYAKV
                     NNPLKR"
     gene            1280..2323
                     /locus_tag="J1Z28_004174"
     CDS             1280..2323
                     /locus_tag="J1Z28_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBA7718941.1"
                     /translation="MTGKLRFAVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLQRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYPPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            complement(2682..4184)
                     /locus_tag="J1Z28_004175"
     CDS             complement(2682..4184)
                     /locus_tag="J1Z28_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA7718942.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSTITTIACGYTERNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTTDEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQHYKQLSQHGADNWKILPGTPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMMGSHPNPCDRLHSWPNHYLEQYPRKVACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGMSVSDSANPVHHDGHIQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVLADSLKPQPLILPVKGK"
     gene            complement(4358..5071)
                     /locus_tag="J1Z28_004176"
     CDS             complement(4358..5071)
                     /locus_tag="J1Z28_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HBA7718943.1"
                     /translation="MAAACVFAVNAGAEEGKSGFYLTGKAGTSVVSLSDQRFLSGDEE
                     ETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKYNVDKDSWSG
                     GYWRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWISAGIGYARIHQKTTGISTWDYEYG
                     SSGRESLSRSGSADNFAWSLGAGVRYDVTPGIALDLSYRYLDAGDSSVSYKDEWGDKY
                     KSEVDVKSHDIMLGVTYNF"
BASE COUNT         1421 a         1202 c         1134 g         1315 t
ORIGIN      
        1 ttgcgaacag aaaagagcta aaaaagtagt ccctgattta caactctctt ttgaacatat
       61 tgcacgacgc tggcatgcca gtaataaaca atgggcacaa tcacacagcg ataaggtact
      121 caaaagcctc gaaacacacg ttttcccctt tatcggcaac cggaacatca caacactcaa
      181 taccccggac ctgcttatcc ctgttcgtgc tgcagaagct aaacaaattt atgaaatcgc
      241 cagtcgtctg cagcaaagaa tatctgctgt aatgcgttat gccgtacagt ctggcatcat
      301 cagatataat cctgctctgg atatggctgg cgcattgacc tctgtaaaac gacagcatcg
      361 ccccgctctt gatctttcac gtctgcctga acttctgtcg cgtattaaca gttataaagg
      421 tcagcctgtc acccggcttg ctgttatgct gaatttactg gtttttattc gctccagtga
      481 actcagatat gcccgctggg ctgaaattga tattgacaat tcaatgtgga ctattccagc
      541 cgaacgcaaa cctctgccag gggtaaaatt ctcacaccgg ggctccaaga tgcgaacacc
      601 acatcttgtg ccactcagca aacaggctgt agccatactg acagaacttc agacatgggc
      661 tggtgaaaat ggtctgatct ttacgggagc acatgacccg cgtaaaccaa tcagtgaaaa
      721 taccgtaaat aaggccctca gagtgatggg gtatgacaca accaaggaag tctgtggcca
      781 tggattccgg gcgatggcgt gcagtgcatt gattgaatcc ggtttatggt caagagatgc
      841 ggttgaacgt cagatgagtc atcaggagcg caacggagta cgtgccgcgt atattcataa
      901 agcagaacat ctggaagaac gtcggcttat gctgcaatgg tgggcagatt ttctggatgc
      961 aaacagggag gagtgtatca gtccgtttga atatgcaaaa gttaacaatc cactgaaaag
     1021 gtgactgaac gccatttaca cacatgctct tcctcttaag gggaagagca cccgcctcaa
     1081 tcaatttctt ttttatgccg gcggaaccat cagccaggct gaggttctca ttcaataact
     1141 caacgtctta ccacagaaca gaattcactg tattatcatg ggcaacgtta atcccggaca
     1201 ataaaaggac accgatgcca ggcatcacaa tttttatagt ctgcagatta tggtccatcc
     1261 ttttttacag gttcagaata tgactggcaa attacgcttt gcagtgaacg ataaccaggg
     1321 ctgtttcata tttccggaaa cctggtttgg ctcgttgctg gatgagtttg aagaactgat
     1381 tgatgcatac gatgcggatg aaatcagcga aacaagttac atcaacaaac tcaggcgcct
     1441 tgcccgacag gagaatgact ttattgatgt acacgctcac ttggcatacg tatttctgga
     1501 gcagaatgcc ccgcgtaagg cgctgaatgc agcccttaaa gggctggcag ttggcaatcg
     1561 tcttatccct gaaggtttca gtggcagaat catctggata catcctgaca accgaccttt
     1621 tcttcgtgcc ctctatgcgg caatcctggc aaatgcacat ttacagcgtc atcaggatgc
     1681 catcatgctc attgaaaaaa tactggatta caatcccgaa gataatcatg gtgcacgctg
     1741 gctactggga ccggaactgt tacgcactgg cgctcatgaa caggcacgcc atatactgca
     1801 ggaacatgca gatgaatttt caccttactg gtatgaactc ggccttctgc attttcttaa
     1861 tggggaactg gtaaaagctg caactgcatt tcgtcgcggc tttgcggcaa atacttacat
     1921 cgcagaaata ctgtgcggaa atctgcatcc atttccactt gctgtatggc ataacttttc
     1981 aggcggtccg gatactgccg aagattatta tgccacatac cccccgttat gggggcaata
     2041 tccggaagca ttgctgtttg taaactggct ctataatcat tcctctgtgc tgcatgaacg
     2101 ggcagaaatc attaaatgcg ccgaaatgct gatgcaggaa gatgatttcg aaatatgcga
     2161 aagtatttta agacagcagg agaagttgcg tgaaagaatt gatgagacgc tttctgagaa
     2221 aattgtacag aaatgcagaa atatgaatgg tgaatatgtc tggccctgga tattgccgtt
     2281 ttcagcggca ggcatgaaac atactggcat acagtatcag tagatattgc attagtgtat
     2341 cctgcacaca agtaataatt tatccaccaa taataacact gttaatgtcc ccttcccctg
     2401 gttgtcagcc aggggttatc ttctgaacat ttcttttgaa aaggataaca cagtaaatta
     2461 tttttatgaa ttatcccatg gactcattaa caccctttca taatgcttta ttgtcaaaca
     2521 cgttatggct gacatcaaaa aaaccggatt tcctctgcca gcgggtaatc acctccccgg
     2581 tgttttcggt tggtctggtt actcctgtct ggttattagc aagataattg ctataaacag
     2641 tggaaaactc atcgtacata atctggtgat gaacattacg cttattttcc cttgaccgga
     2701 agaatcagag gctgtggttt cagactgtct gccagtacat tcctctctcc gttaaaaacc
     2761 ataacgggtt cattatcttc gtctgtcagc ggattgaatg gcggtatatt ttcagtacga
     2821 atgccggtca gccactgaaa aatacctgcg aaatgacggg cactgatttt tctgctgacg
     2881 gactgatgag acgtgatgtc actggcggta ataatcaggg gaacgctgta gcctccttgt
     2941 atatggccat catgatgaac aggattagca ctatcgctga ccgacatccc atggtcagaa
     3001 aagtaaagca tggcgaaatg acgggaatgc cggcgaagga taccatcaag ctgaccgaga
     3061 aagttatcca gtttactgat gctggcgagg taacaggcaa cctttcgggg atactgctcc
     3121 aggtaatgat tcggccagga gtgaagccgg tcacacgggt tcggatgaga ccccatcatg
     3181 tgcaggaata tcaccttcgg agaggattta tccgccagtg cacgttctgt ttcctgtaac
     3241 aacaacatgt catccgtttt acgggaagca aagctgcctt tcttgaggaa aacggtatgc
     3301 tccgcatcag aagcaataac agagatgcgt gtatcatgct cccccagctt tccctgattg
     3361 gatatccacc atgtgctgta tcctgctttt gctgccagcg ccaccacgtt gttgccggag
     3421 tcagggttct gctcatagtc ataaatcagt gtccggctca gggaaggtac ggtactggct
     3481 gctgccgatg tatagccgtc aataaataaa ccgggagctg tattcagcca cggcgtggtt
     3541 ggcacgggat agccatatac cgacatataa tccctgcgca cactctcacc agtgacaata
     3601 acaattgtgt catataacgg tgttcccggc aggattttcc agttgtcagc cccgtgctga
     3661 ctcagttgtt tataatgctg catttcacgc aatgtgtcag ttgtccccac aacagttcct
     3721 ttaaccatcc gcaacggcca gctgtttact gagcataata cgaacagcag cagtgccagc
     3781 cagttacggt gaccgcggtg gtgtgttcgc cagaaaatca ccatgaatac cagaatcgcg
     3841 gcactgacca gaaaatgata aacaggaatc atcccggtaa actccgctgc ctcatcagtt
     3901 gtggtctgca acaacgcgac aataaaactg ttgttgattt taccgtacgt cataccggca
     3961 ggcgcataca gtgcacaaca gaacagaaat aacagagcgg taatggatgt gagggtattt
     4021 ctgtgtgcaa ggagcagaag gagaaacaga agcagcacat ttcctgttgc attcctctca
     4081 gtgtatccgc atgcaattgt ggttattgta gaaacaacaa aaaagaacaa aaacaataaa
     4141 atccggggtg tatttcccgg acaaaacagt tttctgatat tcatcggagt atatcgacaa
     4201 cattattatg aagagaacag gataataaaa atcagaaatt atctgtgaaa cagataaccc
     4261 tgcagtaata gactgcaggg tatttctgtt taattgcaga agatacacaa ttatcttaat
     4321 tgctaaatat acgtattatc gttcaggagc agtgtcgtca gaagttataa gtcacaccaa
     4381 gcatgatgtc atgactttta acatcaactt ctgacttata tttatcgccc cactcgtcct
     4441 tgtaactcac actgctgtca cctgcatcaa gatagcgata gctgaggtcc agagcgatac
     4501 ccggggttac gtcatagcgg acacccgcgc caaggctcca tgcgaagttg tcagcagagc
     4561 ctgaacgtga caacgattcg cgaccactgc ttccgtactc ataatcccag gtactgatac
     4621 cggttgtttt ctggtgaatt ctggcgtagc caatccctgc agatatccat ggtgtgaatg
     4681 cgctgtcatt ccggaagtca tagtacgcat tcagcattag tgtgttgact gacacctcat
     4741 tcttcaggtc atcacgccag taaccacctg accaactgtc tttatctacg ttatacttcg
     4801 aatcagcttt tccacgagcg taaaactcca gttctgtacg aaccggaata ctgaactgcg
     4861 gataaaaatc ataaccagcc gcgataccac cactgaatac cgtatcatgg tcatcgccgc
     4921 ctttatactt tgacgtttct tcctcatctc ctgacaggaa acgctggtct gaaagtgaca
     4981 ccacagaggt accggcttta ccggtcagat aaaaaccgct tttaccttcc tcagcaccag
     5041 catttactgc aaatacacag gctgcggcca cc
//
LOCUS       DADSZD010000237         5062 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.239, whole genome shotgun sequence.
ACCESSION   DADSZD010000237 DADSZD010000000
VERSION     DADSZD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.239"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            194..415
                     /locus_tag="J1Z28_004177"
     CDS             194..415
                     /locus_tag="J1Z28_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718944.1"
                     /translation="MKRLIPVTLLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            846..1871
                     /locus_tag="J1Z28_004178"
     CDS             846..1871
                     /locus_tag="J1Z28_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7718945.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPTEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAFNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1939..3120
                     /locus_tag="J1Z28_004179"
     CDS             1939..3120
                     /locus_tag="J1Z28_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA7718946.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIIGWLFHNTV
                     TNLSNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     LDLVFLSNRGLYIPSPQLGDGFLAFILAVVIAIVLSVGLFRFNKTHQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRRVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            3130..4233
                     /locus_tag="J1Z28_004180"
     CDS             3130..4233
                     /locus_tag="J1Z28_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBA7718947.1"
                     /translation="MTKVLLSHPPRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWVFLQANWVGSTRADCTKSGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRSRYIAVWAVIYPLVVWWLMYGGFLGLDRVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKCFNTERTPH"
     gene            4241..4999
                     /locus_tag="J1Z28_004181"
     CDS             4241..4999
                     /locus_tag="J1Z28_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBA7718948.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERIRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
BASE COUNT         1186 a         1176 c         1275 g         1425 t
ORIGIN      
        1 ttaataagat attcttcttc tcactcttta aaaactttct atagttcccg ctcccttcac
       61 tatttttaca attcacataa attgtcatta ttcattccgc aagatttata acctccagtg
      121 ataaaatgtc attcagctcg ttaataagag agttaactta ttaagcgtta gcgtttatta
      181 ctgaggtaac accatgaaaa gattaattcc tgtgacattg ctcaccgcat tgctggcggg
      241 ctgtgctcac gattcgccct gcgttccggt ttatgacgat cagggtcgtc tggttcacac
      301 caatacctgt atgaaaggca cgactcagga taactgggaa acggctgggg ctattgccgg
      361 cggggcagca gctgttgctg gcctgacgat ggggatcatc gctctgtcga aataactaaa
      421 cagggtgcgg agctccgcgc tctttttaac gatacagtat ctttccataa gataaaatat
      481 tgctatattc tgctgggtaa ttccctgctg atttagcatg ttatatcacg ttatttcgct
      541 gctttcgaaa taacgcatca atttaacttt cacacatccc accagaaata tactcacgct
      601 aacaatcatt attctgctta gtaaaaaacg cgtaaatatt ttgcgccaaa atgtggcgca
      661 tgtttcattt tcgccccatt gcggggcgct gtttttatta tttcctgtct acactcagct
      721 tattgcgacg cgaaatcgtg cgcaaaagct ggcactactt ttgcttataa gaggatggcc
      781 acagacaggt aaaagacgtt tcccaaacgt cctataacga taattttcgc ctcacaggaa
      841 gcattatgaa aaagatgatg atagccacac tggctgccgc cagcgtgctg cttgccgttg
      901 caaatcaggc gcatgctggc gcgacgcttg atgccgttca gaaaaaaggt tttgtgcaat
      961 gcgggatcag tgatggatta cctgggttct cttatgccga tgctgacggt aagttttcag
     1021 gtattgatgt agatgtttgt cgtggtgttg cggcagctgt atttggtgac gacacgaaag
     1081 tgaaatatac cccgctcact gcaaaagaac gcttcaccgc tttacagtca ggggaggtgg
     1141 atttgctctc ccgtaatacg acctggactt catctcgcga tgccgggatg ggaatggcat
     1201 ttaccggcgt cacttattac gacggcattg gcttcctgac tcacgataaa gcgggactaa
     1261 aaagcgcgaa agaactggat ggcgctaccg tctgtattca ggcgggtact gataccgaac
     1321 tcaacgtcgc cgattacttc aaggcaaata atatgaagta cacgccagta accttcgatc
     1381 gctcagacga atcagcgaag gctctggaat cgggccgctg cgatactctg gcctcggatc
     1441 aatcacaact gtatgccctg cgcatcaaat taagcaaccc aactgaatgg attgtcttac
     1501 cggaagttat ctctaaagaa ccacttgggc cggtagttcg tcgtggcgat gatgaatggt
     1561 tctcgattgt acgctggacg cttttcgcca tgctgaatgc tgaagagatg ggcatcaatt
     1621 cccagaacgt cgatgaaaaa gcggctaatc cagcaacgcc tgatatggca catctgctgg
     1681 gtaaagaagg cgattacggc aaggatctga agctggataa taaatgggcc tttaacatca
     1741 tcaaacaggt gggtaactac tcggaaattt tcgagcgtaa cgtaggttca gaaagcccgc
     1801 tgaaaattaa acgtgggcaa aataatctct ggaataatgg cggtattcag tacgcgccgc
     1861 ccgtgcgtta agtcggtgtg aggtaacgag cgtcgctacg gcggcgctct cttcagcgtt
     1921 atgctttccg aggtttctat gtctcatcgc cgctcaaccg ttaaaggctc actctctttt
     1981 gccaacccta cggttcgcgc ctggttattc cagatccttg ccgttgttgc tgttgtcggc
     2041 attattggtt ggttatttca caacactgta acgaatctca gtaatcgcgg cataacatca
     2101 ggatttgctt ttcttgaccg cggggccggt tttggcatcg ttcagcattt gattgattat
     2161 cagcaaggcg acacttacgg acgcgttttt attgtcggcc tgctcaatac gctactggtt
     2221 tctgcattgt gtattgtgtt cgcttctgtt ttgggcttct ttatcggtct ggcgagactt
     2281 tcggataact ggctgctacg aaagctttcc acaatttata ttgagatctt ccgtaatatt
     2341 ccgccattgc tgcaaatctt cttctggtac tttgccgtat tgcgcaattt gcccggacca
     2401 cgacaggcag tgagcgcact ggatttggtg tttctgagca atcgtgggct ttatattccg
     2461 tcgccgcagc tgggagatgg gtttcttgcg tttatcctgg ctgttgttat agctatagtc
     2521 ctttctgttg ggctattccg ctttaataaa acacaccaga taaagaccgg acaactgcgc
     2581 cgcacctggc cgattgccgc agtgttgatc attggtttgc ctttactggc gcaatggctt
     2641 tttggcgcag cactacactg ggatgtccca gcccttcgag gctttaattt ccgcggcggg
     2701 atggttttaa ttcctgaact ggcagcctta acgctggcgc tttcggttta tacttctgca
     2761 tttatcgccg agattatccg cgcagggatc caggcagtgc cttatggtca acatgaagcg
     2821 gctcgatcac tgggattacc caacccggta acgctacgcc gggtcattat tccccaggca
     2881 ttgcgggtga ttattccacc gttaaccagc cagtatctca acatcgtcaa gaactcctct
     2941 cttgccgccg ctattggcta tcccgatatg gtttcgctgt ttgctggcac cgtgctgaac
     3001 caaacggggc aagccatcga gacgatagcc atgaccatgt cggtctatct gattatcagc
     3061 ctgactatct cgctgctgat gaatatctat aaccgccgca tcgcgatcgt tgaacgctaa
     3121 ggagtcatga tgacaaaagt attgctgtct catcccccgc gcccggcgag ccataactca
     3181 agccgcgcga tggtgtgggt gcgaaaaaat ctgttctcca gctggagcaa tagcctgctg
     3241 actattggct gcatatggtt gatgtgggaa ctgatcccac cgttgctgaa ctgggtattt
     3301 ttgcaggcta actgggttgg ctcaacgcgt gccgactgca caaaatccgg tgcctgttgg
     3361 gtcttcatcc acgaacgatt tggtcagttt atgtatggac tttacccaca cgaccaacgc
     3421 tggcgaatta acctggcatt actgattggc cttgtttcta tcgcaccaat gttctggaaa
     3481 atacttccgc atcgcagtcg ctatattgcg gtctgggcgg tgatttaccc actggttgtc
     3541 tggtggctga tgtatggtgg gtttcttggt cttgatcggg ttgaaacccg gcaatggggc
     3601 gggttgacgc taactttaat tattgcatca gttgggattg cgggggcgct accgtggggg
     3661 atcttactgg cgttaggtcg ccgctcccat atgccgattg tgcgtatctt atcggtcatt
     3721 tttatcgagt tctggcgcgg cgtaccgctg attaccgtat tgtttatgtc ttcggtcatg
     3781 ctgccgttgt ttatggcaga aggcaccagt atcgacaaac tgatccgcgc gctagttggc
     3841 gtgatcctgt ttcagtcagc atatgttgcg gaagtcgtgc gaggcggatt gcaggcgctg
     3901 cctaaagggc aatatgaagc ggcagagtcg ctggcattgg gttactggaa aactcagggg
     3961 ctggttattc tgccacaggc gttgaagctg gtaattcctg ggctggtaaa taccatcatc
     4021 gcactcttca aagataccag tctggtgatc atcatcgggt tgttcgatct tttcagtagc
     4081 gttcagcagg caaccgttga tcccgcctgg ttgggtatgt cgacggaagg gtatgttttc
     4141 gccgcgctga tctactggat cttctgtttc agcatgtcac gttatagcca acatctggaa
     4201 aaatgtttta acaccgagcg tacaccgcat tgaggacact atgagccaaa ttttactgca
     4261 acctgctaac gcgatgatta cgctggaaaa cgtcaataaa tggtatggac aattccatgt
     4321 tttgaaaaat ataaatttaa ccgtgcaacc gggcgaacgg atcgttctat gtggcccttc
     4381 aggttccggt aaatcgacaa ccattcgttg tattaatcat ctggaagaac atcaacaggg
     4441 acggatcgtg gtagatggca tcgaacttaa tgaagatatc cgcaatattg agcgcatcag
     4501 gcaggaagtg ggaatggtct ttcagcattt caatctcttc cctcatctga ccgtgttaca
     4561 gaactgtacc ctagcaccga tttgggtacg caagatgcca aagaaagagg ctgaagctct
     4621 ggcgatgcat taccttgagc gggtgagaat tgccgaacat gcccagaagt ttcccggaca
     4681 gatttctggt ggtcagcagc aacgtgttgc catagcacgt tcgctgtgta tgaagccgaa
     4741 aattatgttg tttgatgagc caacgtcggc gctcgatcct gagatggtga aagaggtgct
     4801 ggatacgatg attgggctgg cgcagtcggg tatgacaatg ttgtgtgtaa cacatgagat
     4861 ggggtttgca cgaaccgtcg ctgaccgggt gatttttatg gatcgtgggg aaatagtgga
     4921 gcaagctgca cctgatgaat tttttgcgca tcctaaatca gagcgtacga gggcattttt
     4981 atcgcaggta atccattaat tgaatgttag ttcgaaaagc aaaaaggcca tcctttcgga
     5041 tggcctttcg cttgatttga tg
//
LOCUS       DADSZD010000238         5053 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.240, whole genome shotgun sequence.
ACCESSION   DADSZD010000238 DADSZD010000000
VERSION     DADSZD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.240"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1250
                     /gene="cadB"
                     /locus_tag="J1Z28_004182"
     CDS             <1..1250
                     /gene="cadB"
                     /locus_tag="J1Z28_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBA7718949.1"
                     /translation="ANLASIGGIAIWGWIISIIGAMSLAYVYARLATKNPQQGGPIAY
                     AGEISPAFGFQTGVLYYHANWIGNLAIGITAVSYLSTFFPVLNDPVPAGIACIAIVWV
                     FTFVNMLGGTWVSRLTTIGLVLVLIPVVMTAIVGWHWFDAATYAANWNTADTTDGHAI
                     IKSILLCLWAFVGVESAAVSTGMVKNPKRTVPLATMLGTGLAGIVYIAATQVLSGMYP
                     SSVMAASGAPFAISASTILGNWAAPLVSAFTAFACLTSLGSWMMLVGQAGVRAANDGN
                     FPKVYGEVDSNGIPKKGLLLAAVKMTALMILITLMNSAGGKASDLFGELTGIAVLLTM
                     LPYFYSCVDLIRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILM
                     FYARKMHERQSHSMDNHTASNAH"
     gene            1330..3477
                     /gene="cadA"
                     /locus_tag="J1Z28_004183"
     CDS             1330..3477
                     /gene="cadA"
                     /locus_tag="J1Z28_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBA7718950.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            3536..4993
                     /gene="dtpC"
                     /locus_tag="J1Z28_004184"
     CDS             3536..4993
                     /gene="dtpC"
                     /locus_tag="J1Z28_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBA7718951.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGINTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
BASE COUNT         1176 a         1227 c         1242 g         1408 t
ORIGIN      
        1 ctgcgaacct agcaagtatc ggtggtattg ctatctgggg ttggattatc tctattattg
       61 gtgcaatgtc gctggcatat gtatatgccc gactggcaac aaaaaacccg caacaaggtg
      121 gcccaattgc gtatgccgga gaaatttccc ctgcatttgg ttttcagaca ggtgttcttt
      181 attaccatgc taactggatt ggtaacctgg caattggtat taccgctgta tcttatcttt
      241 ccaccttctt cccagtatta aatgatcctg ttccggcggg tatcgcctgt attgctatcg
      301 tctgggtatt tacctttgta aatatgctcg gcggtacctg ggtaagccgt ttaaccacta
      361 ttggtctggt gctggttctt attcctgtgg tgatgactgc tattgttggc tggcattggt
      421 ttgatgcagc aacttatgca gctaactgga atactgcgga taccactgat ggtcatgcga
      481 tcattaaaag tattctgctc tgcctgtggg ccttcgtggg tgttgaatcc gcagcagtaa
      541 gtactggtat ggttaaaaac ccgaaacgta ccgttccgct ggcaaccatg ctgggtactg
      601 gtttagcagg tattgtttac atcgctgcga ctcaggtgct ttccggtatg tatccgtctt
      661 ctgtaatggc ggcttccggt gctccgtttg caatcagtgc ttcaactatc ctcggtaact
      721 gggctgcgcc actggtttct gcattcaccg cctttgcgtg tctgacttct ctgggctcct
      781 ggatgatgtt ggtaggccag gcaggtgtac gtgccgctaa cgacggtaac ttcccgaaag
      841 tttatggtga agtcgacagc aacggtattc cgaaaaaagg tctgctgctg gctgcagtga
      901 aaatgactgc cctgatgatc ctcatcactc tgatgaactc tgccggtggt aaagcatctg
      961 acctgttcgg tgaactgacc ggtatcgcag tactgctgac tatgctgccg tacttctact
     1021 cttgcgttga cctgattcgt tttgaaggcg ttaacatccg caactttgtc agcctgatct
     1081 gctctgtact gggttgcgtg ttctgcttca tcgcgctgat gggcgcaagc tccttcgagc
     1141 tggcaggtac cttcatcgtc agcctgatta tcctgatgtt ctacgctcgc aaaatgcacg
     1201 agcgccagag ccactcaatg gataaccaca cagcgtctaa cgcacattaa ttaaaagtat
     1261 tttccgaggc tcctcctttc attttgtccc atgtgttggg aggggccttt tttacctgga
     1321 gatatgacta tgaacgttat tgcaatattg aatcacatgg gggtttattt taaagaagaa
     1381 cccatccgtg aacttcatcg cgcgcttgaa cgtctgaact tccagattgt ttacccgaac
     1441 gaccgtgacg acttattaaa actgatcgaa aacaatgcgc gtctgtgcgg cgttattttt
     1501 gactgggata aatataatct cgagctgtgc gaagaaatta gcaaaatgaa cgagaacctg
     1561 ccgttgtacg cgttcgctaa tacgtattcc actctcgatg taagcctgaa tgacctgcgt
     1621 ttacagatta gcttctttga atatgcgctg ggtgctgctg atgatattgc taacaagatc
     1681 aagcagacca ctgacgaata tatcaacact attctgcctc cgctgaccaa agcactgttt
     1741 aaatatgttc gtgaaggtaa atatactttc tgtactcctg gtcacatggg cggtactgca
     1801 ttccagaaaa gcccggtagg tagcctgttc tatgatttct ttggtccgaa caccatgaaa
     1861 tctgatattt ccatttcagt atctgaactg ggttctctgc tggatcacag tggtccacac
     1921 aaagaagcag aacagtatat cgctcgcgtc tttaacgcag accgcagcta catggtgacc
     1981 aacggtactt ccactgcgaa caaaattgtt ggtatgtact ctgctccggc aggcagcacc
     2041 attctgattg accgtaactg ccacaaatcg ctgacccacc tgatgatgat gagcgatgtt
     2101 acgccaatct atttccgccc gacccgtaac gcttacggta ttcttggtgg tatcccacag
     2161 agtgaattcc agcacgctac cattgctaag cgcgtgaaag aaacaccaaa cgcaacctgg
     2221 ccggtacatg ctgtaattac caactctacc tatgatggtc tgctgtacaa caccgacttc
     2281 atcaagaaaa cactggatgt gaaatccatc cactttgact ccgcgtgggt gccttacacc
     2341 aacttctcac cgatttacga aggtaaatgc ggtatgagcg gtggccgtgt agaagggaaa
     2401 gtgatttacg aaacccagtc tactcacaaa ctgctggcgg cgttctctca ggcttccatg
     2461 atccacgtta aaggtgacgt aaacgaagaa acctttaacg aagcctacat gatgcacacc
     2521 accacctctc cgcactacgg tatcgtggcg tccactgaaa ccgctgcggc gatgatgaaa
     2581 ggcaatgcag gtaaacgtct gatcaatggt tccattgaac gtgcgatcaa attccgtaaa
     2641 gagatcaaac gtctgagaac ggaatctgat ggctggttct ttgatgtatg gcagccggat
     2701 catatcgata cgactgaatg ctggccgctg cgttctgaca gcacctggca cggcttcaaa
     2761 aacatcgata acgagcacat gtatcttgac ccgatcaaag tcaccctgct gactccgggg
     2821 atggaaaaag acggcaccat gagcgacttt ggtattccgg ccagcatcgt ggcgaaatac
     2881 ctcgacgaac atggcatcgt tgttgagaaa accggtccgt ataacctgct gttcctgttc
     2941 agcatcggta tcgataagac caaagcactg agcctgctgc gtgctctgac tgacttcaaa
     3001 cgtgcgttcg atctgaacct gcgtgtgaaa aacatgctgc cgtctctgta tcgtgaagat
     3061 cctgaattct atgaaaacat gcgtattcag gaactggctc agaatatcca caaactgatt
     3121 gttcaccaca atctgccgga tctgatgtat cgcgcatttg aagttctgcc gactatggta
     3181 atgactccgt atgctgcgtt ccagaaagag ctgcacggta tgaccgaaga agtttacctc
     3241 gacgaaatgg taggtcgtat taacgccaat atgatccttc cgtatccgcc gggagttcct
     3301 ctggtaatgc cgggtgaaat gatcaccgaa gaaagccgtc cggttctgga gttcctgcag
     3361 atgctgtgtg aaatcggcgc tcactatccg ggctttgaaa ccgatattca cggtgcatac
     3421 cgtcaggctg atggccgcta taccgttaag gtattgaaag aagaaagcaa aaaataatta
     3481 gctcgtacaa gggaagtggc ttgccacttc ccttttttgc tcataaggag aacacatgaa
     3541 aacaccctca cagccgcgcg cgatatacta tatcgtggcg atccaaatct gggagtactt
     3601 cagtttttac ggcatgcgtg ccttactcat tctctatctc acccatcagc ttggttttga
     3661 tgataaccat gccatcagcc tgttcagcgc atatgcttct ctggtttacg ttacccctat
     3721 tctcggcggc tggcttgccg accgcctgct cggcaaccgc actgcagtga ttgccggcgc
     3781 gctgttaatg acccttggcc atgtggtgct gggcattaat acaaattcaa cctttagcct
     3841 gtatctggcg ctggcaatca ttatttgtgg ctacggttta ttcaaatcaa acatcagctg
     3901 tttgcttggc gagctctacg acgagaacga tcatcgacgt gatggcggtt tttcgctgct
     3961 gtatgctgcg ggcaatatcg gttctatcgc cgcgcccatc gcctgcggcc tggctgctca
     4021 gtggtatgga tggcatgttg gctttgccct tgcgggaggc ggcatgttta tcggtttgtt
     4081 gattttctta agtggtcatc gtcatttcca gtccacgcgt agtatggata aaaaagcgct
     4141 cacaagcgtc aaatttgcct taccagtatg gagctggtta gtggtgatgc tctgtttagc
     4201 cccagtattt tttaccctgc tgctggagaa cgactggtcc ggatatttgc tggcgatcgt
     4261 ttgcctcatt gccgcacaaa tcattgcccg catgatgatc aaattccccg aacaccgccg
     4321 tgctctttgg caaattgtat tgttgatgtt tgtcgggacg ttgttctggg tactggcaca
     4381 acagggcggc agtaccatca gcttgtttat cgatcgcttt gtgaatcgtc aggcattcaa
     4441 tattgaagta cctacagcac tattccagtc ggtgaatgct attgcggtga tgctcgctgg
     4501 ggttgttctg gcctggctgg cgtcgccaga aagccgcggc aattcagcat tgcgcgtctg
     4561 gctgaagttt gcctttggct tactgctgat ggcttgtggc tttatgttgc tggcatttga
     4621 tgcccgacat gcagcggctg gcggtcaagc gtcaatgggc gtgatgatat ccgggctggc
     4681 gctaatgggc tttgctgaac tctttattga cccggtggcg attgcgcaaa tcacgcgtct
     4741 gaaaatgtct ggcgtattaa ctggtattta tatgctggca acaggcgcag tcgccaactg
     4801 gctggcaggc gtcgtggcac agcagacgac agagtcgcaa attagcggta tggcaattgc
     4861 cgcttaccag cgattctttt ctcagatggg agagtggacg ttggcttgtg tcgcgatcat
     4921 cgtggtattg gcctttgcta cccgttttct gtttagcacg ccgacgaata tgatacagga
     4981 gagcaacgat taactgtcgt aaaaacaatt ggttatgaat aaattaactt tcagtttatc
     5041 acactagcaa atc
//
LOCUS       DADSZD010000239         4846 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.241, whole genome shotgun sequence.
ACCESSION   DADSZD010000239 DADSZD010000000
VERSION     DADSZD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.241"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            129..953
                     /locus_tag="J1Z28_004185"
     CDS             129..953
                     /locus_tag="J1Z28_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBA7718952.1"
                     /translation="MPENENLLLRLRQSVDGYSRTQQKLGEFVLSDPAKVVYLTITEL
                     ARESGTSEASVTRLCRTLGCKGYNEFKMALALDLRQVQSVEHGGDEIDNVVNESVQAL
                     QDTAKLLDRTLLEAATLALHQAQSVQIYGVAASAILGEYLHYKLLRLGKPAQLFSDMH
                     RAAMNATTLSKNTFVVAISSSGSTRDLLHVVKLARKQGVRVLALSNTPRSPLASLSDI
                     QLVAAKPEGPLSAGALNAKVGVMLLVELLTTSLIALDHKYSDVSQQTASATLPLLL"
     gene            complement(2077..2613)
                     /locus_tag="J1Z28_004186"
                     /pseudo
     CDS             complement(2077..2613)
                     /locus_tag="J1Z28_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304738.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
     gene            2693..3714
                     /locus_tag="J1Z28_004187"
                     /pseudo
     CDS             2693..3714
                     /locus_tag="J1Z28_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3989..4306
                     /locus_tag="J1Z28_004188"
     CDS             3989..4306
                     /locus_tag="J1Z28_004188"
                     /inference="COORDINATES: protein motif:HMM:NF024728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718953.1"
                     /translation="MSRHHSSHPLLHRRAGTILVYIIPLTPDRIDIEQPRRAGVGAGI
                     NVFASVNAAKGNGAEWTETTLDKGKTVTINSGRDMVLNGDQNVTQYVHLTFIRPNILM
                     NGD"
BASE COUNT         1297 a         1154 c         1116 g         1279 t
ORIGIN      
        1 acaattaatt ttaaaaatga aaattaccat caaaaaccaa gacaccgatc atactttttt
       61 aaaatgactg gcatctctcc cctactttcc gccacactat cggcagagaa acgcattaag
      121 gattttgcat gccagaaaat gaaaacctgc tgctgagatt gcgccagagc gttgatggat
      181 atagtcgaac tcaacagaag ctgggagagt ttgtacttag cgatccggca aaagttgtct
      241 acctgacgat taccgaactg gcgcgtgaaa gcggcaccag cgaggctagc gtcacacgtc
      301 tgtgccgaac gctggggtgt aagggctata acgaattcaa aatggcgctt gcactcgatc
      361 tccgacaagt acagtctgta gagcacggcg gtgatgagat tgacaatgtg gtcaatgaat
      421 ccgtgcaagc actacaggac acagcaaaat tactcgacag gaccttgctg gaagcagcca
      481 cgcttgcact gcatcaggct cagtcagtac agatttatgg cgtagcagcc agtgctattc
      541 tgggggagta tttacattac aaactcctgc gtctggggaa acccgcacaa ttgttcagcg
      601 atatgcatcg tgctgccatg aacgccacaa cacttagtaa aaacacgttc gtggtggcga
      661 tttccagttc tggatccacg cgcgatctgc tgcatgtcgt aaaactagcg cgtaagcaag
      721 gtgtgcgtgt actggcgctg agtaatacgc cgcgcagtcc gttagcttcg ctgagtgata
      781 ttcaactggt cgcggccaaa ccggaaggcc ccttgagcgc cggcgcgctg aatgccaagg
      841 tgggagttat gctgttggta gagttactca ccacatcgct gattgcgctg gatcataagt
      901 acagcgatgt gagccagcaa actgccagcg cgaccctacc gctactgctt taaagatata
      961 aaaaaccgca aaagcactga gggatcccca tgaatcagcg ctaataaacc aacaccatat
     1021 tatggtcggt tctggcgaag tgattaagaa cggtgatcag caccattcgt tttaaaatta
     1081 gcgaggagta tcgcaagata ttcttcgcta atgtcagata cgatattacc cgccgcgttt
     1141 taattctgtt ggcctgctgt ctcagatcta cccctttatt ttaagcgtga tagccaataa
     1201 gccaccgcac tattgtgctc agtgttgcca gcaggctcag cccccgtatt tttccagccg
     1261 aacgactgta cctggcacgc acgccgaaac caaaacgctc accttttcat tccggaagtt
     1321 ttgctcaatc ttcattctgt aactgtataa caacatgatt taactagact taaagccttt
     1381 tatgctacta aagattaacc acggttcttt tgccgccgaa ctggcatctt gtatttgtga
     1441 tggtctggct ataccgctgt gggcacgttt attgttccgg cctttctgct ctttttattg
     1501 caaataaaaa caaccattgc accgcgcata ttctgagctg gcaagcgttc ccggcccaga
     1561 tattccggtt tactactggc cagcaacttc tgacacctga accagtattg tcctttactt
     1621 tcaatacgta gctgaatgtt acctctgata cgaccgataa aatcccatcc gagagattta
     1681 atatgtcaga accaagtatt ctggaaccct gcataggtaa ccatctttta tttgttttat
     1741 agcagttgcc aactctgaag caggaacgac atcctcaccg gcagcaatag cagtgagact
     1801 tcattcctga tatcgttatt aacgatccaa gctatcatct gatacgtact tttcacgaag
     1861 cgggtatctg gatatctagg caactgtata atgccgctac ctgagtcatg tgcggcaatt
     1921 gccgccttga gcgcctcgtt ggtctgcctg ccgacgcgta gctataggcc gaattgccaa
     1981 tgaaaaaggc gcttgcgcag cagggggagt aaatccctgt agctggtctt gccgagtagg
     2041 ctgtgcctta gccggcctac tcataggtac aggcgtttag ttaacgggta tcgcctcaaa
     2101 caccagcgtt tccagtgcct gcaacgtaat cactgttgcg tttttatact caacgggcaa
     2161 ggttttatta atgccatata ccgctttcag actaacctgc gccgcgtatc ctgttggtat
     2221 ttcgaaatcc ttcgctaact ctaggtagta ggtttgtggc ttatccgacg ggttgcgcaa
     2281 accaagaatg gctttgtctt tgttccaaga tggccagtcg tacatggcaa gcgccgtcga
     2341 gtcaccacca acccagtggg tatcaaccag cacgctggca ttttccttca accattttgc
     2401 gaccttcgcc aacgtatccc acttcacctt tttcagcaca gatggggtaa tatacagctc
     2461 ctgcagttag gtgctggtca cgaagtaggt ccagacctaa tcgaaaaaat cgcggtccgt
     2521 ttgctctttt tctagtccat aataggcatt ctcggcgctg gctatcccat ggtacatcag
     2581 cgaattcagc gggaacagcg ggcctttacg aatgaaaagg gcttccccaa gatatgcgcc
     2641 tgattaactc ttatgcaatc atggggtaaa catagccccg gagggattcg ttatgaccat
     2701 cacgactgtc ggtatcgatc ttgttaaaaa tgtgttcgct gctcactgcg ttgactaaca
     2761 cggtaaaaac tattctggtt aaaccccaaa atatcggata ctaccattcc ttagctgatt
     2821 gccagtttac ctctctgtgt tatcgggatg gaggtatact ccggggctca ctactggaca
     2881 aggttgtttc gacaatatgg tcatgaacca cgtctgatgg ttgctaaatt tgtatctcct
     2941 tatcgcatgg ctggtaaatc aggcaaaaat gacgcaaata atgctcaggc tatctgtgaa
     3001 gcagttcgtc gcccacatat gcggtttgta acagtgaagg atgagaacca gcaggcgatg
     3061 caatgcctac accgtaccag acagggtttt atcgaagaga aaacagcaac atataaccga
     3121 ttgagaggtt tgatatcgga attcagtgtc atcgccccac aaagtacaga tgcccttcgt
     3181 catatgtttt ctgagcagaa gagttttttg ccacttcagg ttcaacaata cgttcatgac
     3241 ctgctggtac acattgatcg tattgaagat aatattactg aatatgaccg gttttgtccc
     3301 gcatggcaaa agcagatcac cgcagtcagc gactaatgga actgaaaggg attagtacca
     3361 caacggcctg tgcgctggtc gccattatcg gaaatgcaca cgattttaaa atgagcgaca
     3421 actggcaact tggctgggac taacactatc gcaatacagc agtagtggga aatcaacgct
     3481 tggcaggata actaaagctg gcgatacgta tctgcgaatt ctgctggttc aggggggcgt
     3541 tcggttctga ttggtgccga aaaaaggact gattcattca gtcgttgggg ctgatcactg
     3601 gtcgagcgca gaggatactg gcgtaccgtt gtagagatca ccgccaaaaa cgcaaggctg
     3661 tgctgggcat cattgcatta cagtgataat ttcaggctgt actcagccag ctaaagcaca
     3721 aaacaaaata cccatgtgac cgtcaattcg ttgatgataa agggttagac cccgtgaggc
     3781 ctatctggct attgtacaga atatacgtat ccgcttaacg aagaagaacc tcacgcgcgt
     3841 ctttcatcaa ggtccgagtc gatgacgact catcatggcc atttatcgtt ctgttgtctc
     3901 tcccctttat ttttactgaa gagcagacag aaaccataaa caccggcgtt gacatgccgg
     3961 ggaagccctt atattacaat agagctgtat gtctcggcat cacagtagtc atccactgct
     4021 gcaccggcgt gccgggacca tactggttta tataatcccc ctgacgccag atagaatcga
     4081 catagaacaa ccaagacggg ctggcgtcgg tgccggtatc aacgtctttg ctagtgttaa
     4141 tgcagcaaaa ggcaacggtg ctgaatggac cgaaacgacc ctcgacaaag gtaaaaccgt
     4201 caccatcaac agtggtcggg acatggtgct gaacggtgat cagaacgtaa ctcagtacgt
     4261 acatcttacg ttcataaggc cgaacatatt gatgaacggc gactgagctg cagtagtggg
     4321 ctgatttttg gatgtgaata ggaaaaggga agttagtcct tatgactttg gcatgtagtc
     4381 taattcctga tgcttgctat attcgaattt gaaaagccag cttgaactat aacccatact
     4441 tttttataac tgtattattc tggcggctca gaggtgcact atgaaaactt tcgataccca
     4501 acttgcgatg agcattgtat cgcactcaac gggaggtttt agttatgaca cactcgctag
     4561 agccttgatt tctaaaaata aaaatgaaaa tcctgacgac cttcttgaat catgtcgcaa
     4621 tttttgtctc tctcttgagg aaaaaggtat tctgaggcga tgccaaaagt gttctcattc
     4681 tcaagatgag tatttcgagt atattcctca ctagcatcag tccctgaaaa atttttcagg
     4741 gactgataat tccgttcttg tcatcccctt ttttctcctg tagcttgtgt agcacgattt
     4801 aggggggcga ttgtgagcaa tatcaactta ataacaacat gtacaa
//
LOCUS       DADSZD010000240         4799 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.242, whole genome shotgun sequence.
ACCESSION   DADSZD010000240 DADSZD010000000
VERSION     DADSZD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.242"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            98..1762
                     /gene="tsr"
                     /locus_tag="J1Z28_004189"
     CDS             98..1762
                     /gene="tsr"
                     /locus_tag="J1Z28_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBA7718954.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQREASAVVKTVTPATPRKMAVADSEENWETF"
     gene            complement(1805..3076)
                     /locus_tag="J1Z28_004190"
     CDS             complement(1805..3076)
                     /locus_tag="J1Z28_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HBA7718955.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3073..3546)
                     /locus_tag="J1Z28_004191"
     CDS             complement(3073..3546)
                     /locus_tag="J1Z28_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBA7718956.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAILVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            complement(3610..4581)
                     /locus_tag="J1Z28_004192"
     CDS             complement(3610..4581)
                     /locus_tag="J1Z28_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HBA7718957.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
BASE COUNT         1259 a         1148 c         1230 g         1162 t
ORIGIN      
        1 tgaataaaat tactcggcgt catctccgcg ggatattcat aaagtttttc ctttcctggc
       61 cgaaaatctt gcatcggtcc acaggaatag agaaaccatg ttaaaacgta tcaaaattgt
      121 gaccagccta ctgctggttt tggccgtttt tggcctttta caactgactt caggcggtct
      181 gttctttaat gccttaaaga acgacaaaga aaatttcact gttttacaaa ccattcgcca
      241 gcagcaatcc acgctgaatg gcagctgggt cgcgttgttg cagacgcgta acaccctcaa
      301 ccgcgcgggt atccgctaca tgatggatca gaacaatatt ggtagcggtt caaccgttgc
      361 tgagctgatg cagagtgcca gtatttcgct gaaacaggcg gaaaaaaact gggcggatta
      421 cgaagcgttg ccgcgtgacc cgcgtcagag caccgccgca gcggcagaga tcaaacgtaa
      481 ttacgatatt tatcacaatg cgctggcgga gctgatacaa ctgttaggtg caggcaaaat
      541 caacgagttc tttgatcagc cgacccaggg atatcaggac ggtttcgaga agcagtatgt
      601 ggcttatatg gagcaaaacg atcggctcta tgatatcgcc gtcagcgata acaatgcctc
      661 ctacagccag gcgatgtgga ttctggtagg cgtgatgatc gtcgtactgg cggtcatcct
      721 cgccgtctgg ttcggtatta aagcctcgct ggtagcgcca atgaatcgcc tgattgacag
      781 cattcgtcat attgcaggcg gcgatctggt gaaaccgatt gaggtggatg gctctaatga
      841 gatggggcaa ctggcagaga gtttgcgcca tatgcaggga gagctgatgc gtaccgtcgg
      901 tgatgtgcgc aacggggcca atgccatcta tagcggtgcc agcgaaatcg ccaccggcaa
      961 taacgatctc tcttcgcgca ccgagcaaca ggccgcttcg ctggaagaga cggcagccag
     1021 catggagcaa ctgaccgcaa cggtgaagca gaacgccgaa aatgcgcgcc aggccagcca
     1081 cctggcgtta agtgcttctg aaacggcgca acgcggcggc aaagtggtag ataacgtggt
     1141 gcagactatg cgcgatatct ccaccagttc gcagaaaatc gccgatatta tcagcgtaat
     1201 tgacggtatt gccttccaga ccaatattct ggctttgaac gcggcggttg aagcagcgcg
     1261 cgcgggtgag caagggcgcg gttttgcggt ggtcgcgggt gaagtgcgta atctggccca
     1321 gcgcagcgct caggcggctc gtgaaattaa aagcctgatt gaagactcgg tggggaaagt
     1381 agatgttggc tctacgctgg tcgaaagcgc aggggaaaca atggcggaga ttgtcagtgc
     1441 cgtgacccgc gtgacggaca ttatgggcga aattgcttct gcttctgatg agcagagccg
     1501 tggtatcgat caggttggct tagcggttgc tgagatggac cgggtaactc aacagaacgc
     1561 cgcgctggtg gaagaatctg ccgccgccgc tgccgcgctg gaagagcagg ccagtcgcct
     1621 gaccgaagca gtggcagtgt tccggattca gcaacagcag caacagcagc gtgaagcatc
     1681 ggctgtggta aaaaccgtga cgccagctac gccgcgcaaa atggccgtgg cagatagcga
     1741 ggagaactgg gaaacgtttt aacgtaatga tgccggatga gtatttcatc cggcgttttt
     1801 attattaaac caggaacagt gaaatcgccg ggatatatgt caccagcatt agcattaaca
     1861 gtagagccgc cagtaggggc agggattgtt taaagaaatt tcccaccgaa acttgcgtga
     1921 tattacatac cagcagcatc acggtaccta ccggtggcgt gaatgcgcca atcgacaggt
     1981 tgaagatcat gatgatgcca aaatgcaccg gatcgatgcc aaagtgttcg accaccggtt
     2041 ttaatagtgg taccaggatc agcaaaatgg cgtttccttc cagaaacatc cccagcgtca
     2101 gcagcaatat gttgataatc aacaggaaca tgtatttgtt ttcggtcagc gaagtcacaa
     2161 aattcgccac tgcgacacca gcatgttcca ttgagaaaat ccatcccaat gcggcgctgg
     2221 ccatgattac cagcatcacc gatgcggttg cgcgtgccgt ttcgcccaat gctttgacga
     2281 catgagaaat ccgcatttca cgatagataa aaaagccaat cgccagcacg attagcaccg
     2341 ctactgcacc tgcttctgtt ggggtgaaaa tgttagcgcg aataccaccc accacagcaa
     2401 aaatgaggaa cagcgcaggc caggcctgtc ccagcgagaa caccacctct ttgcccgtag
     2461 gccagctttc acgcgagggt ttagcgttag ttttgcgggc gacaaggtag acggtaaaca
     2521 tcagtacgac tgcgcatagc aaaccaggta ccagtccggc gataaacatt tttccgatag
     2581 agacatcggc aaccagaccg taaataatca gggctattcc aggcggtatg atcggggtga
     2641 ctaacgagcc accagcggta atggcggcgg caaacgcgcg attgtagcct ttacgttcca
     2701 tctccggcac catcatgcgc gacagcatcg ccgcgtcagc aaggttagag gcgctgacgc
     2761 cgcccatcat ggtgctgacg agaatgtttg ccacgcccag tccgccgcgc attttcccca
     2821 ccagaacgtt ggcaatgtgc aggatgcgtt cggcaatgcc ggtgtagctc attagtgtgc
     2881 cgagcaagat aaaaaacgga attgccagca atgatgggct ttgcgttggc gcgattagtc
     2941 tttgcacggc aatttcaatc ggtacgctgg agaagaataa aaagtaagcg atgatgccaa
     3001 taaacatcgc cagatagacc cgaatgttga gaaacaaaca gacgatcatt agcggaataa
     3061 tcaataacca actcattgca ttttgtctcc gctatcggat tgttttgtat cgtttattaa
     3121 ccagtagagg gtataaatag ccgttgccag tgcgccgaca gggaccgcca gatcgatcca
     3181 gaaccaggaa accttcagaa tttgtgtcac tttaaactgc gccatttcgg ctagtcggta
     3241 gcccagccag gccatataaa gcagcagtgc gatggagaga agggcgacca gtattgcaat
     3301 aacacgcctg agtttgcctg gaagcatctc catcaggaag ggaatggtca ggtgcgccct
     3361 gtcacgttcc gccaccactc cacctaacat caccacccaa atcatcagca ttcccgacat
     3421 ttcttctgtc cattgaagcg gttggccgat gaaataacgc ataaataccg ctccaattgt
     3481 caccacaacc agaacgaata gcgcaaaggc agcactaaat tctgccactt tgccaaaaag
     3541 acgcatgaaa cctcctgtta gtgcgtgtcc tgcctaagag agatgaggca ggacgctgag
     3601 cattaatgct tattgcagtt gctgttgaat ggtttcgtac agacctggtg tccattccgg
     3661 gaattgagag taaacttcgc gtgctttctt ctggaatggt ccagtatcgg gatagatcac
     3721 ttcgacgccg gctgcctgca tggttttcaa catttcggca tcacgctcaa gagtgagttt
     3781 ctggctatag acacccgctt catagccggt ctggtggatc atctccagtt gttctggtga
     3841 aagggtgcta aagaaggctt caccacctat ccacactgag gtatttgtca ggtagttaac
     3901 cattgtcaga tatcttgcct gctcatacag tttttgccct tgcagcactg aaattgggtt
     3961 ttctacgccg tcaataacgc cctgggttaa tgcgggataa acttcgccta atggcatggg
     4021 agttggtgtt gcccccatcg cctgaatggc ttttatttgc atgacgttat taggaacgcg
     4081 aatttttaaa cctgcgagat cgtcaacggt gcgaatgggt tttttcgaaa taatctgtcg
     4141 agtgccatag agataattat tcattaccac atgaattccc tttttcttca gactttcatt
     4201 tttttgttta aaccagtcgc tttcatatat tttgaacagt ttttgcggat cgtcagtcag
     4261 atacggtcca aacaaaatcc ccaaatcggg ttcataatcg gcaagaaaag ccacatcact
     4321 caaggttatg acattcatgc ccatcatggc ttgctcggtg acatcctgtt ttgatcccaa
     4381 ttgggagctg ggatagagcg caagtgttat ttcgccatta ctttttttat ttaataggtc
     4441 tgcccagtaa cgcatgacca catccagggg ttctccaggg ttattttcat aggcaacttt
     4501 tattgaaata ggttttgcct ggattattga cggtaaaaaa agggtactga cagtaattag
     4561 catcgctatt attttgctca tctttactta ctccatgcta aggaaataaa actccaacat
     4621 tgttgtcggg agtgaaacct tatgaaaaag cagtcattac tgaaaagtag tcagcgaata
     4681 gcagaatggc tgcagatgta tcataaattc gtgcaccggg taatactttg ctgcttttaa
     4741 tcggttttta atgcattttt tgattgtata tcttaaagtt atgtaatgca tgacgttac
//
LOCUS       DADSZD010000241         4782 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.243, whole genome shotgun sequence.
ACCESSION   DADSZD010000241 DADSZD010000000
VERSION     DADSZD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.243"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            221..1051
                     /locus_tag="J1Z28_004193"
     CDS             221..1051
                     /locus_tag="J1Z28_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBA7718958.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVASQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            1083..2018
                     /locus_tag="J1Z28_004194"
     CDS             1083..2018
                     /locus_tag="J1Z28_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBA7718959.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFARLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            2111..2353
                     /locus_tag="J1Z28_004195"
     CDS             2111..2353
                     /locus_tag="J1Z28_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBA7718960.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2343..3794
                     /gene="panF"
                     /locus_tag="J1Z28_004196"
     CDS             2343..3794
                     /gene="panF"
                     /locus_tag="J1Z28_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBA7718961.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3806..4687
                     /gene="prmA"
                     /locus_tag="J1Z28_004197"
     CDS             3806..4687
                     /gene="prmA"
                     /locus_tag="J1Z28_004197"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBA7718962.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
BASE COUNT         1087 a         1191 c         1251 g         1253 t
ORIGIN      
        1 aaaataactt acaaaaaata acttaattta tttttcactt tgcaagtgaa accaatggaa
       61 accctgcata aatatgaata tattcattta tttttcaatg tattaagcta ttttcgttgc
      121 aatttttgac ccctgatttt cttttcgaat tgtcgatatg attgaaggat aactttcaaa
      181 gaaaaataca aggggctata gtgttacgca ttaaaggcca atgaagagtg ttaacgtgcc
      241 gagaaaatcg tctaacctac tgaaacgcag attaaaaatt gcagaaattg ttgcctccca
      301 gggtgaaatc aaagttgatg acttatctgc gcaacttggt gtctctggcg tgacgatccg
      361 cggcgatctc agttatctgg agcaacaggg atatttaaag cgatcatttg gcggtgctat
      421 tgcgacacca ctctcctctg aaccagaagc gatacaaccg atcgcgccat tgtctctggc
      481 acaacaaatg gaaatagcac gacattgcgc acgattaatt cgcgatcgtg acacgctatt
      541 tcttggacat gggacaatct gccgtaagat cattccattg ctcagcgaag tgaaaaaact
      601 gcgtctgatc accaacgatc ccgaacatgc gctgctggca aatcagttta ttgatggcga
      661 aataatactg gctggcagtg aaatgctgcg ccccgacacc gcactggccg gaaaacagct
      721 tgaacaggca cttcagcatt tcaccattac acatagcatt ctggaaatca gccatctgga
      781 tgctgatggt actttaaata tcaacgttcc cagactcgcc gctgcctggc agctctgttt
      841 cgatcatgcc cagaataaga cggttatcgt tgccgccgat cctgcgccat caacggctgc
      901 tatcgggcat ttgcatcagg cagaaaatgt gatcgtcagc cgcagcatta acgaacaata
      961 tcagcaacaa ttgcagtcgg ctgatttcgt tatcctttat accagtaatg agtgctttac
     1021 ctggtccaat cgcgaaaggc ttcaggaatg agaggggttt tgtgacaata actatctgcg
     1081 ctatgggcga attgctggcc gagtttttgt cccgcaaccc acatcaaaaa ttcactcagc
     1141 ctggggagtt tatcgggcca tttcccagcg gtgcgccagc aatttttgct gctcaggtgg
     1201 caaaactgtc ccatcgggcc atcttctttg gatgtgttgg taatgatgat tttgcccgac
     1261 tcattataga gcgtctccgt catgaaggtg tcattaccga tgggatccat gttatgaaca
     1321 atgccgtcac aggtacggcg ttcgtgagtt atcaaaatcc ccagcagcgg gatttcgtct
     1381 ttaatatccc taacagcgcc tgcggtttgt ttactgccga gcacattgat aaggatctgc
     1441 ttaaacagtg taaccatctg catattgtgg gctcatcgtt gttctcattt cgcatgatcg
     1501 atgtcatgcg taaagcaata acgacgatca aatcggctgg cggcaccgtt tctttcgatc
     1561 ccaatattcg caaagagatg ctgagtattc ctgaaatggc gcaggctctc gattatttga
     1621 ttgaatatac ggatattttt atccccagcg aaagcgaact ccctttcttc gcgcgtcaca
     1681 aaaatctgtc agaagaacag attgttagcg atcttctcca cggcggcgta aaacatgtgg
     1741 cgataaaacg cgcccagcgt ggggccagct attacaagct taaaaacggt acattacacg
     1801 cccagcatgt tgcaggtcac gatatcgaaa ttatcgatcc aacgggtgca ggcgactgct
     1861 ttggcgcaac gtttatcact cttttcttat ccggtttccc ggcacacaag gcgctgcaat
     1921 atgcaaatgc cagcggcgcg ctcgccgtaa tgcggcaagg tccgatggaa gggatatcct
     1981 cactggcaga cattgaagac tttttgcagc agcactgaac ggcaattttc tgtcagatca
     2041 ttttccctgc ctgaagactt gccgtagaat tgcccgtttc tcgtatcccc ccatgaggag
     2101 gaccccttcc gtggacactc gttttgttca ggcccataaa gaggcgcgct gggcgctggg
     2161 gctgaccctt ttgtatctgg cggtatggtt agtagccgct tacttacctg gcgttgcccc
     2221 cggttttacc ggctttccgc gctggtttga gatggcctgc atcttgacgc cgctgctgtt
     2281 tattggactg tgctgggcga tggtgaaatt tatctatcgc gatatcccac tggaggatga
     2341 cgatgcagct tgaagtaatt ctaccgctgg tcgcctatct ggtggtggtg ttcggtatct
     2401 ctgtttatgc gatgcgtaaa cggagcactg gcaccttcct taatgagtat ttcctcggca
     2461 gccgctctat gggcggtatt gtgctggcga tgacgctcac cgcgacctat atcagtgcca
     2521 gttcgtttat cggcgggcct ggagctgctt ataaatacgg cctgggctgg gtattgctag
     2581 cgatgattca gcttcctgca gtctggcttt cgctcggtat tctcggcaag aagttcgcaa
     2641 ttcttgcgcg ccgctacaat gcggtgacgc tgaacgatat gctgtttgcc cgctaccaga
     2701 gtcgtcttct ggtatggctg gcgagtttga gtttgctggt tgcgttcgtt ggtgcgatga
     2761 ccgtgcagtt tatcggcggt gcgcgcctgc tggaaaccgc ggcgggtatt ccttatgaaa
     2821 ccgggctgct gattttcggt atcagcattg cgttgtatac cgcctttggc ggctttcgcg
     2881 ccagcgtgct gaacgacacc atgcaagggc tggtgatgct gattggcacc gttgtgctgc
     2941 ttattggcgt ggtacatgcc gcaggtggtt taagtaatgc ggtagacacg ctgcaaacca
     3001 tcgatccgca actggttacg ccacaaggcg ctgacgatat tctgtcgcct gcctttatga
     3061 cgtcgttctg ggtactggtg tgctttggcg ttataggcct gccgcatact gcggtgcgct
     3121 gtatctctta taaagacagc aaagccgtac atcgggggat cattatcggt acgattgtag
     3181 tcgcaatcct gatgttcggt atgcatctgg ccggagcgtt aggtcgggcg gtgatccccg
     3241 atctcaccgt accggatctg gtgatcccaa cgttaatggt aaaagtgctg ccaccgtttg
     3301 ctgccgggat cttcctggct gcgcctatgg ctgcgatcat gtcgacaatt aacgcccaac
     3361 tgctgcaaag ttccgctacg atcattaaag atctctatct gaatatccgt ccggatcaaa
     3421 tgcaaaacga gacgcgtctg aagcggatgt cggcggtgat cacgttggtt ctcggtgcgt
     3481 tgctgctgct tgccgcctgg aagccgccag aaatgatcat ctggctgaat ttgctggcct
     3541 tcggtgggct ggaagccgtt ttcctgtggc cgctggtgct gggtctttac tgggaacgcg
     3601 ccaacgccaa aggcgcgcta agtgcgatga tcgttggcgg cgtgctgtat gccgtactcg
     3661 cgacgctgaa tattcagtac ctgggcttcc accctatcgt gccctcgtta ctactaagtt
     3721 tgctggcttt cctggtcgga aaccgtttcg gtacatccgt cccgcaagct accgttttga
     3781 ctactgataa ataaagagtt ttgccatgcc ttggatccaa ctgaaactga acaccaccgg
     3841 cgcaaacgcg gaagatctta gcgatgcgct gatggaagcg ggtgccgttt ctatcacttt
     3901 tcaggatacc cacgatacgc cagtatttga gccgctgccg ggcgaaacgc gcctgtgggg
     3961 cgacaccgat gtgattggtc tgttcgacgc tgaaaccgat atgaacgacg tggtggcgat
     4021 tctggaaaac catccgctgc tcggcgcggg cttcgcgcat aaaatcgaac aactggaaga
     4081 taaagactgg gagcgcgaat ggatggataa tttccacccg atgcgctttg gtgaacggct
     4141 gtggatctgc ccaagctggc gtgatgtgcc ggacgaaaac gccgtcaacg tgatgttaga
     4201 cccagggctg gcgtttggta cgggtaccca tccaactacc tctctgtgcc tgcaatggct
     4261 cgacagcctc gatttaaccg gtaaaacggt catcgacttt ggctgtggtt ccggcattct
     4321 ggctatcgcg gcgctgaagc tgggtgcagc aaaagccatt ggtattgata tcgacccgca
     4381 ggcgattcag gccagccgcg acaacgccga acgtaatggc gtttctgacc gtctggaact
     4441 ctacctacct aaagatcagc cagaagaaat gaaagccgac gtggtggtcg ctaacatcct
     4501 tgcaggccca ttacgtgaac tggcaccgtt aatcagcgtc ctgccggttt caggtggctt
     4561 gctgggcctt tccggtattc tggcaagcca ggcagagagc gtttgtgaag cttatgccga
     4621 cagcttcgct ctggacccgg tcgtggaaaa agaagagtgg tgccgcatta ccggtcgtaa
     4681 gaattaacct tcgcatcgcc gtagggtgac gcaaggcgaa gtgcgagcaa ggtcacaaaa
     4741 ggcacgttaa tttgccgatt atttacgcaa atttgcgtgc ca
//
LOCUS       DADSZD010000242         4776 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.244, whole genome shotgun sequence.
ACCESSION   DADSZD010000242 DADSZD010000000
VERSION     DADSZD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.244"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(158..1000)
                     /gene="purU"
                     /locus_tag="J1Z28_004198"
     CDS             complement(158..1000)
                     /gene="purU"
                     /locus_tag="J1Z28_004198"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBA7718963.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(1050..1508)
                     /locus_tag="J1Z28_004199"
     CDS             complement(1050..1508)
                     /locus_tag="J1Z28_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBA7718964.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            1621..2526
                     /gene="rssA"
                     /locus_tag="J1Z28_004200"
     CDS             1621..2526
                     /gene="rssA"
                     /locus_tag="J1Z28_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBA7718965.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            2618..3631
                     /gene="rssB"
                     /locus_tag="J1Z28_004201"
     CDS             2618..3631
                     /gene="rssB"
                     /locus_tag="J1Z28_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBA7718966.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNSGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISQCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            3833..4741
                     /gene="galU"
                     /locus_tag="J1Z28_004202"
     CDS             3833..4741
                     /gene="galU"
                     /locus_tag="J1Z28_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBA7718967.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
BASE COUNT         1253 a         1075 c         1203 g         1245 t
ORIGIN      
        1 ggggtaatgc tttttactgg cctgctccct tatcgggaag cggggcgcat catatcaaat
       61 gacgcgccgc tgtaaagtgt tacgttgaga aaaatgaact ggttgcgtaa ttttcatccg
      121 taacggatta aagctaacca gttatttttg ctggcgatta aagaataatc gtccgattac
      181 cgtaaacaaa gacgcgctgc gccagtactt tgtatagcgc acgacttaag acgtttttct
      241 cgacgtcacg acctgcgcgc atcatatctt cagctgtgta ggtatgatcg acatgaataa
      301 cgtcctgcat gatgattggg ccttcgtcca gattgtcatt cacatagtga gcggttgcgc
      361 caataatctt cacgccacgc tcataggcct ggtgataagg acgtgcgccg ataaacgctg
      421 gcaggaatga atggtgaata ttgatgatct tattcgggaa gcgtgccaca aattccggcg
      481 ttaatacccg catatacttc gccagcacca catagtcagg ttgataagca tcaatggcat
      541 ccgccatctt ttgatcgtgc tcgttgcggc ttaacccttc atggcttacc agctcaaacg
      601 gaatatcaaa ccgctcaacc agagaacgta aagtatcgtg gttaccaata actgccgcga
      661 tttcgacatc caggccaccg taattggctt tcatcaacaa atcgccaagg caatgcgctt
      721 ctttagtgac cagaattact atccggcgac gaccggcagg attcagctca cgcacggagc
      781 cttctggcaa tgcgctatcg agatccgcca gcagggtgga atcattaaaa atcccttcca
      841 gttccgtgcg cataaaaaag cgcccggtac gatgatctac aaattcattg ttctgcacga
      901 tatttaactc gtgcttgtag caaatattgg taatacgtgc gatcagacct ttttggtccg
      961 gacaaatagt acgcagaact ttacgttgga gtgaatgcat tgctggaaaa accttgttga
     1021 gagtgtttgc taaaccgtac cgtcaaccat tattggccgc agcacttttt aaatttttta
     1081 cctgaaccac aagggcaggg atcgttgcgg ccaaactgcg gacgtgtacc gtcaatatag
     1141 taccattgac cgttttcttt caaaaatcgc gaacgttcaa ttattgcacc tgttttgcca
     1201 ccctcggtaa atcgagcaac aaaactgaca aatccgatat tgccgccgtc ctgccagcaa
     1261 tgttcaaata cggtcaatcc caaccattca gtatgcgcaa atccggcaat caattcggct
     1321 cgtaacgccg ctgctccaca agatggatgc caggtcttta ttaaataatc tgcgtcttgc
     1381 atcacaaaag cgcagtaacg cgaacgcatg agatgttctg gatcaggtgc aaccttttca
     1441 ccagacacat aagggtggca acataggcta tactcgacag cactaccaca gggacaaagc
     1501 tgagacacaa ataatctccc tggaaacaat aacggcgtat taaccgcctg agtagcacta
     1561 tgttaaccga gcagtagcaa tgtggctacg attgcattcc aggggaatct tgcgggaata
     1621 atgagaaaga taaaaatagg gctggcgctg ggatctggcg cggcaagagg ttggtcgcat
     1681 attggcgtaa ttaatgcgct aaaaaaagtg ggtattgaaa ttgatatcgt tgcaggatgt
     1741 tcaattggtt cgctggtggg cgctgcctat gcatgcgatc gattatctgc gctggaagat
     1801 tgggtgacct ctttcagtta ttgggatgtt ttacgcctga tggatctctc ctggcagcgc
     1861 ggtgggttac tgcgcggcga gcgtgtcttc aatcaatatc gcgaaataat gccggaaaca
     1921 gagatcgaaa attgttcccg tcgcttcgcg gctgttgcca ccaatttaag tactgggcgt
     1981 gaattatggt ttactgaagg cgatctccat cttgctattc gtgcatcatg cagtattcca
     2041 ggactaatgg cacccgttgc gcataacggc tactggctgg ttgatggcgc tgtagttaac
     2101 ccaattccta tttccctcac gcgtgcattg ggggctgata ttgtgatagc ggtcgacctg
     2161 cagcacgatg ctcatttgat gcaacaagat ttgctctcct ttaatgtcag tgaagaaaat
     2221 agcgagaatg gtgattctct gccgtggcat gcgcgtctga aagaaaggtt aggcagcata
     2281 acgacacgtc gggcggtgac agcgccaacg gcaacagaga ttatgaccac ttctatccag
     2341 gtgctggaga accgccttaa aaggaaccgc atggcaggtg atccgcccga tattctgatt
     2401 caacctgttt gcccgcaaat atctacgctt gatttccatc gcgcgcacgc tgccattgcg
     2461 gccgggcagc tggcagtgga aaagaaaatg gacgaacttt tgccgttggt acgcaccaac
     2521 atttgaccag aatttttatc tacacttaag ttaattctga caggcgcagg tggcaatagc
     2581 atgccactat tgagtaaagc cagtcagggg agagaacatg acgcagccat tggtcggaaa
     2641 acagattctc attgttgaag atgagcaggt atttcgctcg cttctggatt cgtggttttc
     2701 ctcattggga gcgacaacgg tactggcggc tgatggggtg gatgcccttg agttgctggg
     2761 aggtttcact ccagacctga tgatatgtga tatcgcgatg ccacgaatga acgggcttaa
     2821 actgctggaa catatacgca acagtggtga ccagacaccg gttctggtga tatctgccac
     2881 tgaaaatatg gcagatattg ccaaagcgtt acgtctgggc gttgaagatg ttttgctgaa
     2941 accggttaaa gatctgaatc gcttgcgcga gatggttttt gcctgtctct atcccagcat
     3001 gtttaattcc cgcgttgagg aagaggaaag gctttttcgc gactgggatg caatggttga
     3061 taaccctgct gcagcggcga aattattaca ggaactacaa ccgccagttc agcaggtgat
     3121 ttcccaatgc cgggttaatt atcgtcaatt agttgccgcg gacaaacccg gcctggtgct
     3181 tgatattgcc gcactttcgg aaaacgatct ggcattttat tgccttgatg tcacccgagc
     3241 tggacataat ggcgtacttg ctgccttgtt attacgcgca ttgtttaacg gattattaca
     3301 ggaacagctt gcacaccaaa atcaacggtt gccagagttg ggcgcgttat tgaagcaggt
     3361 aaaccattta ctccgtcagg ccaatctgcc ggggcagttt ccgctattag ttggctatta
     3421 tcatcgcgaa ctgaaaaatc tcattctggt ttctgcgggt ctgaatgcga cgttaaatac
     3481 cggcgaacat caggtgcaaa tcagtaatgg tgttccgtta ggcactttag gtaacgctta
     3541 tttgaatcaa ttgagccagc gatgcgatgc ctggcaatgc caaatatggg gaaccggtgg
     3601 tcggctgcgc ttgatgttgt ctgcagaatg agcaaacgat aacacgggct aaatttgcat
     3661 tacctgctaa tgtcggctgg tggtactatc gtcgctattc gtataagtaa ttgtcttaat
     3721 tatgctaact cgcctccttt tcagaactta gccccttcgg ggtgctgata tactgggatg
     3781 cgatacagaa atatgaacac gttcaaaaca cgaacagtcc aggagaattt aaatggctgc
     3841 cattaatacg aaagtcaaaa aagccgttat ccccgtagcg ggattaggaa ccaggatgtt
     3901 gccggcgacg aaagccatcc cgaaagagat gctgccactt gtcgataagc cattaattca
     3961 atacgtcgtg aatgaatgta ttgcggctgg cattactgaa attgtgctgg ttactcactc
     4021 atctaaaaac tctattgaaa accactttga taccagtttt gaactggaag caatgctgga
     4081 aaaacgtgta aaacgtcaac tgcttgatga agtgcagtct atttgtccgc cacacgtgac
     4141 tattatgcaa gttcgtcagg ggctggcgaa aggcctggga cacgcggtat tgtgtgctca
     4201 cccggtagtg ggtgatgaac cggtagctgt tattttgcct gacgttattc tggatgaata
     4261 tgaatccgat ttatcacagg ataacctggc agagatgatc cgccgctttg atgaaacggg
     4321 tcatagccag atcatggttg aaccggttgc tgatgtgacc gcatatggcg ttgtggattg
     4381 caaaggcgtt gaattagcgc cgggtgaaag cgtaccgatg gttggcgtgg ttgaaaaacc
     4441 aaaagcggat gttgcgccgt ctaatctcgc tattgtgggt cgttacgtac ttagcgcgga
     4501 tatttggccg ttgctggcaa aaacccctcc tggagctggt gatgaaattc agctcactga
     4561 cgcaattgat atgctgatcg aaaaagaaac ggttgaagcc tatcatatga aagggaagag
     4621 ccatgactgc ggtaataaat taggttacat gcaggccttc gttgaatacg gtattcgtca
     4681 taacaccctt ggcacggaat ttaaagcctg gcttgaagaa gagatgggca ttaagaagta
     4741 acatccgtat cggtgttttc cacgaaacgg cgttga
//
LOCUS       DADSZD010000243         4768 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.245, whole genome shotgun sequence.
ACCESSION   DADSZD010000243 DADSZD010000000
VERSION     DADSZD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.245"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            114..812
                     /locus_tag="J1Z28_004203"
     CDS             114..812
                     /locus_tag="J1Z28_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBA7718968.1"
                     /translation="MIFQKIARLLKSEINGYSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPPIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVAHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(857..1756)
                     /gene="eamA"
                     /locus_tag="J1Z28_004204"
     CDS             complement(857..1756)
                     /gene="eamA"
                     /locus_tag="J1Z28_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBA7718969.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1951..3138
                     /gene="ydeE"
                     /locus_tag="J1Z28_004205"
     CDS             1951..3138
                     /gene="ydeE"
                     /locus_tag="J1Z28_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBA7718970.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLMAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            3265..3360
                     /gene="mgtS"
                     /locus_tag="J1Z28_004206"
     CDS             3265..3360
                     /gene="mgtS"
                     /locus_tag="J1Z28_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBA7718971.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3579..4469)
                     /gene="dgcZ"
                     /locus_tag="J1Z28_004207"
     CDS             complement(3579..4469)
                     /gene="dgcZ"
                     /locus_tag="J1Z28_004207"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBA7718972.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSSMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         1310 a         1146 c         1011 g         1301 t
ORIGIN      
        1 taatacagca ctaaagttgt tcaacatcag ggtgataatt aaacgactcg gccatacgat
       61 aaaccaatca ggactcaact ccttgttttc aatggctctt aaaaggtgca aaagtgattt
      121 ttcaaaagat tgcccgctta cttaaatccg agatcaatgg ctattcatgg catgttggtg
      181 atttgttgcc gtcagaagct gaactcgctg ttcgttataa tgtttcgcgt aataccctgc
      241 gtaaggcatt atccttgctg gaaggcgaag gaattattca tagaaagcat ggttcaggaa
      301 catacattca aaaaaagaat tttgttgcac acattgatca catgaacagc ttcagtgaaa
      361 ttgcgcataa aagcggcaag gaggcaggaa gtcagattat gaaatttgaa gtgcaggatg
      421 cttctccccc tattgcgact gagctgaatt tagtgactgg tgagcaggtt tattacataa
      481 agagactgcg attcattgag gataatgcag cacaattaga agaaacgtgg atgtcagtgg
      541 cacgttttcc tgatttaacc gtagcacata tgcaaaaatc caaattttcg tatattgaga
      601 acgaatgcgg gatcaaaatc attggcacct ttgaaacttt ctcccctact tttcctaccc
      661 cagaaatcgc cagtatttta cggatcagcc cacgggatcc catacttaaa attcagaccc
      721 aggctgtgga cagtaactct attccgctgg attattcgtt actttacagc aatattttcg
      781 agttccaggt aaagtacttt ttcccgcgat aaaagcagac gcacagagtg ctgacatcac
      841 attctgtgcg tatggtttag cctctaacct ttaccgcttt acgccaccgc aagccaaata
      901 cattgatata cagcccggtc ataatgagca ccgccccgaa aaattgtaga cccgttaagc
      961 gttcatccaa caatagtgcc gcacttgcca gtcctactac gggcaccagt aacgataacg
     1021 gtgcaacccg ccaggtttca tagcgtccca gtaacgtccc ccagatccca taaccaacaa
     1081 ttgtcgccac aaacgccaga tacatcagag acaagatggt ggtcatatcg atagtaacca
     1141 gactgtgaat catggttgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     1201 ttgggattaa agcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     1261 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgccgcc agggtcaaca
     1321 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     1381 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg cagtcgctca ccgaaagtaa
     1441 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     1501 caggcatacc gaagttaatg gcacaaaaaa gaaaagcaaa ctgcgcaaaa ctgatggtta
     1561 atccataccc cagcagcaaa ttcagcggta ctttcggtcg tgcgacaaaa aagatagccg
     1621 gaaaagcaac cagcataaag cgcaaaccgg ccaacatcag cggtggcatg ttatgaagcc
     1681 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     1741 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     1801 tgaatttact tttctttaac agttgattcg ttagtcaccg tttacgacgg cattaatgcg
     1861 caaataagtc gctatacttc ggatttttgc catgctattc ctttacatct ctaaaacaaa
     1921 acataacgaa acgcactgcc ggacagacaa atgaacttat ccctacgacg ttctaccagc
     1981 gcccttcttg cctcgtcgtt gttattaacc atcggacgcg gcgctacgct gccgtttatg
     2041 accatttact tgagtcgcca gtacagcctg agtgtcgatc taatcggtta tgcgatgaca
     2101 attgcgctca ctattggcgt cgtttttagc ctcggttttg gtatcctggc ggataagttc
     2161 gacaagaaac gctatatgtt aatggcaatt accgccttcg ctagcggttt tattgccatt
     2221 cctttagtga ataacgtgac gctggtggtg ctcttttttg cccttattaa ctgcgcctac
     2281 tccgtttttg ctaccgtgct gaaagcctgg tttgccgaca atctttcgtc caccagcaaa
     2341 acgaagattt tctcaatcaa ctacaccatg ctcaacattg gctggaccat cggtccgcca
     2401 ctcggcacgc tgttggtgat gcagagcatc aatctgccct tctggctggc agctatctgt
     2461 tccgcgttcc ccatgctttt cattcaaatt tgggtaaagc gcagcgagaa aatcatcgcc
     2521 acggaaacag gcagtgtctg gtcgccgaaa gttttattac aagataaagc actgttgtgg
     2581 tttacctgct ctggctttct ggcttctttt gttagcggcg catttgcttc atgcatttca
     2641 caatatgtga tggtgattgc tgatggtgat tttgccgaaa aggtggtcgc ggttgttctt
     2701 ccggtgaatg ctgccatggt ggttacgttg caatattccg tgggccgccg ccttaacccg
     2761 gctaacatcc gcgcgctgat gacagcaggc accctctgtt tcgtcatcgg gctggtcggt
     2821 tttatttttt ccggcaacag cctgctattg tggggtatgt cagctgcggt atttactgtc
     2881 ggtgaaatca tttatgcgcc gggcgagtat atgttgattg accatattgc gccgccagga
     2941 atgaaagcca gctatttttc cgcccagtct ttaggctggc ttggtgctgc gattaaccca
     3001 ttagtgagtg gcatagtact taccagtctg ccgcctttct cgctgtttat catcttagcg
     3061 ttagtgatcg ttgttgcgtg ggtgctgatg ttaaaaggaa ttcgtgcaag accgtggggg
     3121 cagcccgcgc tttgttgatt taagtcgaac acaataaaaa tttaattcag ccttcgttta
     3181 ggttacctct gctaatatct ttctcattga gatgaaaatt aaggtaagcg aggaaacaca
     3241 ccacaccata aacggaggca aataatgctg ggtaatatga atgtttttat ggccgtactg
     3301 ggaataattt tattttctgg ttttctggcc gcgtatttca gccacaaatg ggatgactaa
     3361 tgaacggaga taatccctca cctaaccggc cccttgttac aactgtgtac aaggggcctg
     3421 atttttatga cggcgaaaaa aaaccgccag taaaccggcg gtgaatgctt gcatggatag
     3481 atttgtgttt tgcttttacg ctaacaggca ttttcctgca ctgataacgg atcgttgaca
     3541 cagtagcatc agttttctca atgaatgtta aacggagctt aaactcggtt aatcacgttt
     3601 tgttcgtcaa taaacataca gcgatttctt ccggtttgct taccctcata cattgcccgg
     3661 tccgctcttc caatgaccac atccagaggc tcttcaggaa atgcgcgact cacacctgct
     3721 gtcacggtaa tgttgatatg cccttcagaa tgtgtgatgg catggttatc gactaactgg
     3781 caaattctga cacctgcacg acatgcttct tcatcattag ccgctttgac aataatgata
     3841 aattcttcgc ccccgtagcg ataaaccgtt tcgtaatcgc gcgtccaact ggctaagtaa
     3901 gttgcgaggg tgcgtaatac tacatcgccg attaaatgcc cgtaggtatc attaaccaat
     3961 ttaaatcggt ctatatccaa caacattaaa taaagattca gaggttcagc attgcgtaac
     4021 tgatgatcga aggattcatc aagaacccga cgacccggca atcccgtcag aacatccata
     4081 ctgctacgga tcgtcagcaa ataaattttg taatcggtta atgccgcagt aaaagaaagc
     4141 aacccttcct gaaaagcatc gaaatgcgcg tcctgccagt gattttcaac aatagccagc
     4201 attaattccc gaccacagtt atgcatatgt tgatgggcag aatccattag ccgaacgtaa
     4261 ggtaattcat cgttatcgag tggccccaga tgatcaatcc accgaccaaa ctggcacagt
     4321 ccataagaat ggttatccgt tatttctggc ttactggcat ctctcgcgac cacgctgtga
     4381 aacatactta ccagccactg gtagtgggca tcgatagcct tattgagatt taacaagatg
     4441 gcatcaattt ccgttgtctt cttgatcatt gccactcctt tttcacagtt ccttgtgcgc
     4501 gctattctaa cgagagaaaa gcaaaattac gtcaatattt tcatagaaat ccggagttat
     4561 gagcatctct gagataacat tgtgatttaa aacaaaatca gcagataaaa aagtgtttaa
     4621 ttctataaat taactctgca ttatcgcaat taaaaggatg acaaatagca taacccaata
     4681 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaactgagt
     4741 gacccgaacg acggccggcg cgcttttc
//
LOCUS       DADSZD010000244         4756 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.246, whole genome shotgun sequence.
ACCESSION   DADSZD010000244 DADSZD010000000
VERSION     DADSZD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.246"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            93..1067
                     /gene="hemB"
                     /locus_tag="J1Z28_004208"
     CDS             93..1067
                     /gene="hemB"
                     /locus_tag="J1Z28_004208"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBA7718973.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALNLAEKKILR"
     gene            complement(1107..1958)
                     /gene="tauD"
                     /locus_tag="J1Z28_004209"
     CDS             complement(1107..1958)
                     /gene="tauD"
                     /locus_tag="J1Z28_004209"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBA7718974.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQVITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1955..2782)
                     /gene="tauC"
                     /locus_tag="J1Z28_004210"
     CDS             complement(1955..2782)
                     /gene="tauC"
                     /locus_tag="J1Z28_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBA7718975.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAVLLTVWWAVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRILLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2779..3546)
                     /gene="tauB"
                     /locus_tag="J1Z28_004211"
     CDS             complement(2779..3546)
                     /gene="tauB"
                     /locus_tag="J1Z28_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBA7718976.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQIQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3559..4521)
                     /gene="tauA"
                     /locus_tag="J1Z28_004212"
     CDS             complement(3559..4521)
                     /gene="tauA"
                     /locus_tag="J1Z28_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBA7718977.1"
                     /translation="MAISSRNTFLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDIPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1110 a         1370 c         1210 g         1066 t
ORIGIN      
        1 cttcttgtca acatcgcgac aactttcgta aaacacaccc agcctgcttc aggtatacta
       61 tgctcctcga ttccacaaac atcaggcaga ccatgacaga tttaatccaa cgccctcgtc
      121 gcctgcgcaa atctcctgcg ctgcgcgcta tgtttgaaga gacaacactt agccttaacg
      181 acctggtgtt gccgatcttt gttgaagaag aaattgacga ctacaaagcc gttgaagcca
      241 tgccaggcgt gatgcgcatt ccagaaaaac atctggcacg tgaaattgaa cgcatcgcca
      301 acgccggtat tcgttccgtg atgactttcg gcatctctca ccataccgat gaaactggca
      361 gcgatgcctg gcgggaagat ggactggtgg cgcgtatgtc gcgcatctgc aagcagaccg
      421 tgccagaaat gatcgttatg tcagacacct gcttctgtga atacacatct cacggtcact
      481 gcggtgttct gtgcgagcat ggcgtcgaca acgacgcgac tctggaaaat ttaggcaagc
      541 aagctgtggt tgcagctgct gcaggcgcag acttcatcgc cccttctgcc gcgatggatg
      601 gtcaggtaca ggcgattcgc caggcgctgg acgctgcggg ctttaaagat acagcgatta
      661 tgtcgtattc gaccaagttc gcctcctcct tttatggccc gttccgtgaa gctgccggaa
      721 gcgcattaaa aggcgaccgc aaaagctatc agatgaaccc aatgaaccgt cgtgaggcga
      781 ttcgtgagtc actgctggat gaagcccagg gcgcagactg cctgatggtt aaacctgccg
      841 gagcatacct cgacatcgtg cgtgagctgc gtgaacgtac tgaattgccg attggcgcgt
      901 atcaggtgag cggtgagtac gcgatgatta agttcgccgc gctggcgggt gctatagatg
      961 aagagaaagt cgtgctcgaa agcttaggtt cgattaaacg tgcgggtgcg gatctgattt
     1021 tcagctactt tgcgctgaat ttggctgaga agaagattct gcgttaatga tcattatgcc
     1081 gggttatcca acccggcatt tcccccttac cccgcccgat aaaacggaat atccccaaga
     1141 atcgtcgccc gatgcataat ccgccgctgt ggcaggtaat ccgcattggc atagtgctga
     1201 gtcacgcggt tatcccaaat cgcaatatca ttcggttgcc agcgccagcg cacctgaaac
     1261 tccggtttag tgatatgggc aaacaaaaaa cctaacaagg cttcgctctc tttctcgctg
     1321 acatcaacaa ttcgtgtggt aaaaccttcg ttcacaaaca gcgcctgttt accggtcacc
     1381 ggatgcgttc gcaccaccgg atgtagcaac ggtggatttt tcgcgaccgc ctcacgccag
     1441 cgttgatgct cagcctcggt tttgcggtac ttgtattccg ggaacgattt acggaaatca
     1501 tgctccgcac gcaacccgct caacagctgg cggaagggaa cagagagcgc ctcatatgcg
     1561 gcaataccac tggcccagag cgtatcgccg ccggtcgagg gtaactcttt cgccgccaga
     1621 atcgcccctg cgggtggcgt ttgaataaat gtcacatcgg tgtgccagtt gtcgttatcc
     1681 ggcggattat cgttatgggt atccaggacg atgatctcgt caaccccttc agcatgaggg
     1741 taaacagggt gaatatgcag ctcgccaaaa cgctgggcca gcgcgcgttg ctgctgcggc
     1801 gtaataactt gatcgcgcag aaacaccacc tgatggcgca gcaccgcatg gtaaagctgt
     1861 tcaaactgat tatcgcttaa cgggcgcgtt aagtcggccc ccgtaatttg tgcgccaata
     1921 tacggcccca gcggggtaat gctcagacgt tcactcattg tacttctcca tgccagggcg
     1981 tcaggcggcg ctgtaacgcg cgcagaccca gttctaaaag aaaggcgata atcgcaatca
     2041 ccgcgatccc cgccagcacc acgtcagtcg cgagaaattc acccgctgac tgaaccataa
     2101 atcctaaacc gcgcgtcgcg gcaatcagct ccgccgccac cagcgtagac cagcccacac
     2161 ccagaccaat gcgtaatccg gtgaggattt ccggcagcgc gccgggcaaa atgacaaacc
     2221 acagcacctg cgcacggctg gcacccagcg actgggcggc acgaatgcga acctgctgcg
     2281 cgcttttcac ccccgccagc gccgacatcg ccaccggcgc aaaaatcgct aaatagatca
     2341 gtaagatctt cgaggtttcg ccaataccaa accagatcac catcagcggc aaataagcca
     2401 gcggcggcac cggacgataa agctcgatta tcggatccag aatgccgcgc accgtcgggc
     2461 ttagccccat ggcgatcccg accggaatac cgatcaacac cgccgcgagt agcgccagca
     2521 aaatgcgcgt caggctggct gccagatgct gccacagcgt ggcgtccata aatccctgcg
     2581 gcccggcaat ggtgagcagt tttgccagta cctgttgcgg tggcggcaaa aacagcgggc
     2641 taatcagttg cagcgccgcg actgcccacc atacagtgag caaaaccgct aacgtaccaa
     2701 tgcttaaggt cacctgacgc gagagcggcc agcgccattt cagccgatgc gaatgcagtt
     2761 tttcattaat gagcacactc atgagaacgc ctcccgttgc tcaaatacgc ggcttaaaac
     2821 atattcgcgc atggcgatga attgtggatc ggacttgatg ctgcggctcg actctcccgc
     2881 aacaaagcgg cgagcaaagt tgagtggcag ccgctccagc acacggcctg gaccgggtga
     2941 aagcagaacc agttcagtcg ccataaacac cgcttcttct atatcgtggg taatcaacag
     3001 cacctgcttt cccgtctcct gccagagttt cagcagcagg gtttgtatct ggtcgcgggt
     3061 gaaggcgtcc agcgcaccaa acggttcgtc gagtaataac agctggggat ttgccgccaa
     3121 cgcgcgggca atccccaccc gctgacgttg accaccggaa agctgccaga tgtagcgttt
     3181 ttctgcgcct tccagcccga cttttttcac catctggtgt gcgatttcca gtcgctgcat
     3241 tttctctaca cctgccagtt gcaggccgaa cgccacgttg tcctgtacat tgcgccacgg
     3301 taacaaccct tcattctgaa aaactacgcc acgctctgct cccggtccct gaataccctt
     3361 acccgccagt tgaatgctgc catgctgata aggcacaaaa ccagcaatca aattcagcaa
     3421 cgtagttttg ccgcagccag acggccccag aactaccaga agctcgccgc tttccagcgt
     3481 caggttgata tcttccagtg ccggtttgcc gccataatcg gcgtaaagat gagaaatttg
     3541 cagcatctgc gcctcctttt attgcacgaa gcgcgaggta acgtactggc tgtaatcatt
     3601 cgctacagcc gggactttgc cctgctcttt caaaaactgc gcggtgtcga tgatcgcttt
     3661 gttcaccggt ccggtcagtt ctgccgtttg ctgctgcggc gtcagatagg tattcccctt
     3721 caccagcccc ggaatgtcac cttccggcac gccgcttaac cgcgccagtt tgctgatgtt
     3781 ttccggctgt ttcagccacg cgtctgggtt agcaatgtac ggttgctgag catcgatggc
     3841 gcttttagcg aacgctttca cgacctcagg atgtttctcg gcaaaatctt tccgcacgac
     3901 ccagacgtcc agggtcggcg cgccccactg cccgacctgt tcagaatcgg tcagcacctt
     3961 gccatctttt tccagggcgt taaccgccgg tgcccagaca taagcaccat caatatctcc
     4021 ccgctgccat gcggcgataa ttgcgggcgg ctgcaggttc acaatctcaa cttgcccagg
     4081 tttaataccc cagtgtttca gcgccgccag caggctgtag tgggtggtgg agataaacgg
     4141 tacggcgatg cgtttaccaa tcaagtcttc cggtttactg atagttttct ttaccaccag
     4201 cgcttcggag ttacccagtt ttgacgccag caagaagact tcaatcggca cctgttggct
     4261 ggttgcaacc gctaacgggc tggaaccgag gttgccgatt tgcacgtcgc ccgaagccag
     4321 cgcccgcacg atgctggctc cactgtcaaa cttacgccag tccacggttg ctccgctttc
     4381 tttagcaaag gtgttgtcgg cctgagccac tttcgccggt tcggcggagg tttgatacgc
     4441 cacggtgacg ttcaccgctt gcgcctgaaa agcgatgaat gccagtgcgg caagaaatgt
     4501 gttacgcgat gaaattgcca tgattgtctg ctcccccgtc ttgttatggg agcagtattc
     4561 aggaataaaa acattcatta aaagaattag tcgttatcgt acagatgatt ttattcttag
     4621 caaaaaagcg gcaaaggaaa gattaaatat gcaattacgc ccactctgat aactcagtaa
     4681 attactgcac ttcattttat taatgcataa acaagcaggt tctaatgtct gttattttat
     4741 taacagcaac acatat
//
LOCUS       DADSZD010000245         4729 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.247, whole genome shotgun sequence.
ACCESSION   DADSZD010000245 DADSZD010000000
VERSION     DADSZD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.247"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..677
                     /locus_tag="J1Z28_004213"
     CDS             <1..677
                     /locus_tag="J1Z28_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBA7718978.1"
                     /translation="AALGQMATEIAHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNL
                     KHIISVIERMTQLISELKAFASRHRVPKGSADVIKVMYSAVALLNHSMEKNNIERRIK
                     APSMPLFVNCDELGLEQIFSNLISNALDSMEGSSYKRLDIAIRQANNKVIITIKDSGG
                     GFAPEVVDRIFEPFFTTKRRGMGLGLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPE
                     WGEEHE"
     gene            670..2028
                     /locus_tag="J1Z28_004214"
     CDS             670..2028
                     /locus_tag="J1Z28_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBA7718979.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(2017..3090)
                     /locus_tag="J1Z28_004215"
     CDS             complement(2017..3090)
                     /locus_tag="J1Z28_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HBA7718980.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(3102..4601)
                     /locus_tag="J1Z28_004216"
     CDS             complement(3102..4601)
                     /locus_tag="J1Z28_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HBA7718981.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSVILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
BASE COUNT         1308 a         1152 c         1123 g         1146 t
ORIGIN      
        1 tagcggcgct tggacagatg gcaaccgaaa ttgcccatga acaaaatcaa ccgttagccg
       61 ccattcacgc acttactgat aacgcgcgta ctatgctaaa aaaagagatg tatccgcagg
      121 ttgaacagaa tctgaaacac attatttcag tgattgagcg gatgacgcag ctcatttccg
      181 aacttaaagc atttgcctcg cgccatcgcg tacctaaagg ttctgccgat gtcatcaaag
      241 tgatgtatag cgccgtggcg ttacttaatc acagcatgga gaaaaataac attgagcgac
      301 gaataaaagc cccatccatg ccgttatttg tcaattgcga tgagctcggt cttgaacaga
      361 tattcagtaa tttaattagc aacgccttag attctatgga aggtagctct tacaaacgac
      421 tggatatcgc cattcgccag gcaaataaca aagttattat taccattaaa gacagcggtg
      481 gcggttttgc acctgaagtt gtcgatcgca tatttgaacc attttttacc actaaacgta
      541 gaggaatggg gttgggactg gcaatagtca gcgaaattgt ccgaaattcg aacggcgcac
      601 tccacgccag taatcatcct gaaggcggcg cagtaatgac attaacctgg cctgaatggg
      661 gagaagaaca tgaataataa atccagtgaa attactatag tttatattga agatagtgac
      721 gatgtacgtt ttgcctgtga acaaacgctc acgctggctg gttatcgtgt tatttcctgt
      781 tgcgatgcag aacacagtat tccccttatt caaagtcagg ccaacattat tatactgacc
      841 gatgtcaggt tacctggaat atcaggacta gagctcctca gttacatcaa cgaaatggat
      901 agcaaaattc cggttatttt aattaccgga cacggtgacg tggaaatggc ggtggatgcc
      961 atgcgtaatg gtgcattcga tttcattgag aaaccaagta gctcagataa attattgagt
     1021 attattgcca gagcggtaga aaaacggcga ttggttttag aaaatcagca attactggcg
     1081 aatctgcaac aggaaaatgg cccggtgctg attggtcgta gtccgcagat gcaacaactg
     1141 cgtaaaatga tccttaatgt cgcagatacc ggtgccgatg tccttattta tggtgaaaca
     1201 ggttgtggta aagaagtcgt cgccagaatg ctgcatcact ggagtacccg ccgtcagggg
     1261 cagtttgttg ccttaaactg tgcaggctta ccagaaacgt tgtttgaaag cgaaatattt
     1321 ggtcatgagg cgggcgcatt taccggtgcg gttaaaaagc gtattggaaa aattgaacat
     1381 gcaaatggtg gtacgttatt tctcgatgaa atagagggaa tgccatcagg aatgcaggta
     1441 aaactgctac gtgtattgca ggaaagaacc attgaacgat taggtgccaa tcaattaatt
     1501 ccggtgaatt gccgtgttat tgcagcgacc aaggaagatt tactgcgtcg cagcgaagaa
     1561 catctgtttc gtctcgatct ctattatcgt ctgaatgtgg ttagcctgaa tattccacct
     1621 ctacgccagc gacgagaaga tatccctgaa ctgttttact ggttcgccag tcaggcggca
     1681 caaaaatata atcgtcctct gcctgatatt tctcccatgt tactggcctg gttgcagtca
     1741 caaagttggc ctggcaatgt acgtgaactt aaacacaatg cagagcgttt tgttctcggt
     1801 ttactgaccc accaccagcc agtgccaatg actcagcaag aagagagtgg ccttaccgcc
     1861 tgcattgatg ctttcgagaa aaagttaatc gaagatatgc tgcgacaaac ggaaggccag
     1921 gtcagtttga cagcgcggct attacagctg ccgcgcaaga ctttatatga caaactcaac
     1981 aaacatcaga ttcaaccgca agtttatcga ccagaatcat ccagctaatg ctgattcctg
     2041 aggatgtaac cccaaatcga tacctttgcg ggcgtaatgt tcaactaccc aggaccagaa
     2101 tgataccggg atcgtaatca tcttcgcatt gcgttcccgg ttggcagctt ctggcatacc
     2161 ggggtaaatc accggattgc cgccaggttg aggctgcgta gctaacagat aatccgcaaa
     2221 gaactgccct ttctcccctg ccagatcggt gaattgatca ggatcgataa tcaacgcaaa
     2281 aagatgatta tgcgcagatc catccgtctg atgatcttcg gcgatcgtgt caccaccaga
     2341 taagatcccc gccatcattt caatcatcag tgccaggcca aatcctttat gctcaccaaa
     2401 tggcagtaac gcccctggag gattgctgaa taacacccca ggatcattgc ttggattccc
     2461 ctgtgcatca atcaattgtc ccggcgcggc ctgcttcccg gtggacgaca ttacccgtgc
     2521 tttatttagc gccatgctac tggtggcaaa atcaaccaga atcggcggac gcccatcact
     2581 gaacggccag gcaaagcaga aagggttagt ggtcatacgc ggttccgcac cgccccacgg
     2641 ggcgaccagt gcaggacgcc cggtgacgtt actcatggcc agcatcgcca ggccttgatc
     2701 agcaaccatt tgcccgtaat gccccatacg ccctaaatga tgcgcatggc acaacgttaa
     2761 caggcaaaaa ccgcggtgtt ttgcttgttc gatggcagca tttaccgcca cttttccggc
     2821 gtgttgacca aagccattgt gggcatgaaa acgttgtaaa ttaccttcac ttgttaagca
     2881 ttccgggcgg gcattcgcgg ttacatcgcc acgagcgata ttttccagat atttcggtag
     2941 cagcgtgacg ccgtggctgg caaagccata acgttccgct tcaatcatat gttccgccac
     3001 atcctgagcg atatcggcat cacagccatt aagttccagc agcccggcag ctaaccaacg
     3061 cactttttca acagcaattt gtaatgacat cgttggtttc ctcacattag acctaaaatt
     3121 ttccaccagg tggcggcaaa cagtagcaac aacaggtagc cgattaccgt caggtacagt
     3181 cccacacggg taaactggcg tggcgtgaag gtatccgtac ccatacacac catattttgt
     3241 ggcgcgttaa tcggcaggat gaagccaaaa ctgacactga aggcgagcag aatggtcatg
     3301 ccgaccgggt taacgccgag ttctggcggc agactgctta acaggctaat caggatgggc
     3361 agcaatgcgg cagttagcgc agtggcactg gcaaacccca ggtgaataat tatcaagaaa
     3421 gcggcgagaa tcgcgaagat ggctaatgat ggcaagccat cgagaccaaa acctttcacc
     3481 acatagttcg ccatccatga agctgcctgc gtatcaagca gcgtggaccc caggctgata
     3541 ccaataccaa acatcagcaa cgtgccccac tgcacacgtt tttcgacctc tttccagctc
     3601 attacaccga tgcctggcag caacataatc gccagtccgg caagggtgac agaggtggta
     3661 tcaatactgt gtaattttcc gccagtagac caaaagagca gtaacaacag cgaaataccg
     3721 atcaagcgtt tttctttacc ggtggttggc cctaattctg cgagtgcttt tttaatggcc
     3781 tcgctccccc cttcaacagc ttccgtttct ggcggcaaca atttacgtgc gaggaaatag
     3841 aggatcaccg acatggtgag gctccagggc gcgcccgcta acagccagtc aagccagctg
     3901 acagaatgcc cggcgccgaa agttttattg ataaaaccga tagaaagcag gttttgcgcc
     3961 gccgaagttt gaataccaac gttccagata ctggtcgcct gggcgataac aatcatcatt
     4021 gacgccgcta aacgtgaatg tttatccacc ttaaatgccg caatgactcc catcataatc
     4081 ggcaccacgc aagccgtacg ggcagtggca ctgggtacga ccaggctcag gacgatggtc
     4141 accacaatgg caccgataat aatgctgcgg ctgctggcac ctattttcga catagtaaac
     4201 agcgcaatgc gtttatccag cccggtaatg gtcattgccg ccgcgataaa cattgctgct
     4261 gccaccagcg ccagagcaga attagaaaaa cccgacagag tcattttcag cgctttcgcg
     4321 gtgccaagga tcgtgtccgg gtgattcata tccggggcaa aaccaaccat aaagataatc
     4381 agtgcggaaa taacaattgc actggcggtg taatccatcg cttcgctgat ccagacaata
     4441 atggcaaaca ccagaatggc gatcatattt tgtccagcga cgggcaaggt atcgcccagt
     4501 ggcagaaggg taataataat tccggcaatg atcgccaacc acaggccggg attagggata
     4561 attcgacgct tttgtggtgc gccatgggat attgatgaca tattcacctc attgtgaaaa
     4621 ataaaaaacc tgcctggcta ttgcaggagc tatgccatct ttaatttttt aataacgctt
     4681 tgttatttaa tgattttatt atttattctt tttattttgt gcgcggttt
//
LOCUS       DADSZD010000246         4660 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.248, whole genome shotgun sequence.
ACCESSION   DADSZD010000246 DADSZD010000000
VERSION     DADSZD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.248"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            135..554
                     /locus_tag="J1Z28_004217"
     CDS             135..554
                     /locus_tag="J1Z28_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008323638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HBA7718982.1"
                     /translation="MAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTSQDGRREFETS
                     ELIRAYGELKRNETPERHTAGQAEKPHDQQTERILRELSELKQCLTLMLEDKQAQDTE
                     RRRQEAEREQLQNEIAQLRQALEQERKRGFWSRLFGR"
     gene            635..1063
                     /gene="exc2"
                     /locus_tag="J1Z28_004218"
     CDS             635..1063
                     /gene="exc2"
                     /locus_tag="J1Z28_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HBA7718983.1"
                     /translation="MKRSGGTRYMYLSALITVSAALTACTPKTSLEQHTRHYVYASDD
                     GFDPNFYTKKADTTRMMIPFFQQFREMGVKDKAAGVSAETAQQRIKEFHSEEFLQSLR
                     TTTTFAGRKYTNSDMPSPEKMKLLADTISAIYLDGYEGRQ"
     gene            1894..2085
                     /locus_tag="J1Z28_004219"
     CDS             1894..2085
                     /locus_tag="J1Z28_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBA7718984.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGETL"
     gene            complement(3060..3191)
                     /locus_tag="J1Z28_004220"
     CDS             complement(3060..3191)
                     /locus_tag="J1Z28_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HBA7718985.1"
                     /translation="MRGLVFCALILILPGCQANYIRDVQGGTVAPSSSSAATGIEIR"
     gene            complement(3203..3682)
                     /locus_tag="J1Z28_004221"
     CDS             complement(3203..3682)
                     /locus_tag="J1Z28_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718986.1"
                     /translation="MIKRLLFLYVALLFLSEGAYASVTVFIAPSWERNTDSVNEIYKY
                     IDDINSNNHVIDQAYVEPIRKELGVYRDYIQKKLINFNGKGVCKLSITTGSTGVKDIE
                     PPDVVLLNSLAENSSLDCKKLYREIQSIVDNDPSLLFPEKIKSNGLLSIDMIIVMGR"
     gene            complement(3765..4523)
                     /locus_tag="J1Z28_004222"
     CDS             complement(3765..4523)
                     /locus_tag="J1Z28_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7718987.1"
                     /translation="MSGIENSNQGPGWHDHNTPYGTIHSYDGDSHSGYGAHSGGNGGN
                     RHEGISDKTHISIPSTRNYNGYYMSMCVNNLKAHEGFKNTMYKDSEGNITVGIGHLLS
                     TPDMAASLPFTRTHTWHGHGDDMEKEVAASKKEITDAFNNFKKDSRQVPMNLHLSNDA
                     VIGLCISDVQTTEKGLRSLYSGYDGFSNSRKTALVDMGFNLGIPKLSRNFPNFNAAVN
                     RGDWESAARESHRNLHQRGDQRNKDTADQLRDKH"
BASE COUNT         1330 a         1127 c         1000 g         1203 t
ORIGIN      
        1 aaaaaaacag ccactcacac catgtgccat ccccgccaga tgccacaaaa accagcacaa
       61 acattaccgt tctccggcct cagtatgttt tactgagacc atgagatgag acacctatgg
      121 gacactgtca ctttatggca tggcacacac tccgggacgc actgaaaatg acgggcagat
      181 cgcgttcaca gttttaccgt gatatgcgcg caggtcttgt cagttaccgt acaagccagg
      241 acggacgacg ggagtttgaa actagtgaac tgatccgggc atacggcgaa ttaaagcgga
      301 atgagacacc agaaaggcac acagcgggac aggcagaaaa acctcatgat cagcagacag
      361 aacgcattct ccgggagctg agcgagctga aacaatgcct gacgctgatg cttgaggata
      421 aacaagccca ggatacggag cgccggcgcc aggaagcaga acgggaacaa ctacagaatg
      481 agatagccca gctcaggcag gcgctggagc aggaaaggaa acggggattc tggtcccggt
      541 tgtttggtcg ctgaacgctg tcagagactg atgataagat agtcttctga taataactca
      601 ccgaaaataa atacattaag gtaaggagac tcgcatgaaa cgttctggcg gcacacgcta
      661 tatgtatctt agcgccctga taactgtgtc agcagccctc acagcctgta ccccaaaaac
      721 cagcctggaa caacataccc ggcattatgt ttatgcttca gatgatgggt tcgatcctaa
      781 cttctacacc aagaaagctg acaccacacg gatgatgatt ccgttctttc agcagttcag
      841 ggagatgggg gtgaaagaca aagcagccgg agtatcagca gaaacggcac agcaacgcat
      901 aaaagaattc cactctgaag aattccttca gtcactgaga accacaacca cctttgcggg
      961 cagaaaatac acgaacagtg atatgccatc accggaaaaa atgaagctgc tggctgacac
     1021 gatttctgcg atttatctcg atggctacga gggaagacag taagtggatt taccataatc
     1081 ccttaattgt acgcaccatt aaaacgcgtt gagcgcgatt ctgacgcagg cacagcaaaa
     1141 agaaatgttt acccccagga aaaccggaaa aatgcgtcag gatcgttttc agggcgttgc
     1201 atgactatgc acgaaactga atagcgatct gtttgggcgc atacggtgcc catgaggcac
     1261 attttcggac attttcagtt cgtgaatccc tggctcattg ctacatatcc gggcagacac
     1321 ccctggcagc gcccccaggg gtgttatgta aaaaaaggcg acgcgcccct aggggcgccc
     1381 ctacattaat taactgtttt ttctacagat agtcagtact gaccctaagt agggtcgtca
     1441 tagcgtcgcc atagggtcgt cgacgacgtg cttttgtata aatctgaata agaaaataca
     1501 tttttaaaac cataacaaca tgatttaaaa agcaaatcag caaaaagttg gttttgcgtg
     1561 gggtgtggac atttcagaca tgagaacaga ctcgcgtttt tccggagtat cagcggaata
     1621 tctgattaaa aaacactcgc cgcagagcgc gattttatgc aacagctgct gtactgcaca
     1681 gggattattt atcagttgat atgctgtgcc atgatattgc tattgccgaa agggacgtcg
     1741 ttttacggcg ttttccctat cctgcatcgc actggccgcg ttcgaatgtt tgcgcccctg
     1801 actaaccgct cactcagctt tatcactctc gcctgcgtca cagttatcat gctcccatga
     1861 acagaaattc ccttacacgg agacatcaga ccagtgacca aacaggaaaa aaccgccctg
     1921 aacatggccc gcttcatcag aagccagaca ttaaccctgc tggagaagct caacgaactg
     1981 gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgccga tgagctttac
     2041 cgcagttgcc tcgcacgttt cggggatgac ggtgaaaccc tctgacatat gcaggtcccg
     2101 gagacggtca cagcttgtct gtgagcgaat gccgggagca gacaagcccg tcagggcgcg
     2161 tcagcgggtt ttagcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga
     2221 gtgtatactg gcttaatcat gcggcatgtc cggtgtgaaa tgccgcacag atgcgtaagg
     2281 agaaaatgcc agtataggcg ctcttccgct tcctcgctca ctgactcgct acgctcggtc
     2341 gttcggctgc ggcgagcggt gtctactcac tcaaaagctg taatacagtt atccacagaa
     2401 tcaggggata atgccggaaa gaacatgtga gcaaaaagga aaaaggccgc gcagctgacg
     2461 tttttccata ggctccgccc ccctgacgag catcacaaaa attgacgctc aagtcagagg
     2521 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2581 cgctctcctg ttccgaccct gccacttacc ggatacctgt ccgcctttct cccttcggga
     2641 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcttggtgta ggtcgttcgc
     2701 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2761 aactatcgtc ttgagtccaa cccggtaaga cacgacttaa cgccactggc agcagccact
     2821 ggtagaggat gtacaagaaa ttgctgatac gtagttcttg aagtgaagga ccgactgcgg
     2881 ctacactgaa aggacagatt tggtggcagc ggtcttgtac accctgttac ctcgggttag
     2941 aaagcctgag aaacttctaa ccttcgaaag aacccacgcc tgagaacgtg ggttttttcg
     3001 tttacaggca gcagattacg cgtagaaaaa aaggatctca agaagatcct ttaatctttt
     3061 tacctaattt cgattccagt agctgctgag ctagaagatg gcgcaacagt ccctccttga
     3121 acatccctga tataatttgc ctgacagccg ggcaatatta aaattagcgc acaaaaaacc
     3181 aaaccacgca tagaattgta ttttatctcc ccataacaat gatcatatca attgataaca
     3241 aaccatttga ctttattttc tcaggaaata aaagtgacgg atcgttatca acaatacttt
     3301 gtatttctct atacagtttt ttacagtcta atgatgagtt ttccgccaat gagtttaaca
     3361 aaacaacatc tggaggctca atatctttaa cacctgtact tcctgttgtt atcgataatt
     3421 tacaaactcc cttcccatta aagttaatta gttttttctg gatataatct cgatataccc
     3481 ctaactcctt cctaataggt tctacatatg cttgatcgat gacatggtta ttactattta
     3541 tatcatcaat atatttatat atctcattaa ctgaatctgt attcctttcc caacttggag
     3601 caatgaatac tgttacagat gcataagcac cttctgataa gaacaataaa gcaacatata
     3661 agaataataa ccgcttgatc atataatcca ttcccattat aggttgatgt atttcatctt
     3721 taatataaga tgaaatacat ctcatatcta tatgttatgg tctattaatg tttatcccgc
     3781 aactgatctg ctgtatcctt gtttctctga tcgcctcgtt gatgaagatt tcgatggctt
     3841 tcacgtgctg cagactccca atccccacgg ttcacagcag cattaaagtt cggaaagttt
     3901 ctcgatagct taggaattcc taagttaaat cccatatcca ctaaggcagt ttttctagag
     3961 ttagaaaaac catcataacc agaatataaa gaacgcaagc ctttctctgt agtctgaaca
     4021 tcggaaatac ataatccaat aacagcatca tttgataaat gcaagttcat tggtacttgt
     4081 ctagagtctt ttttaaagtt attaaaagca tcggttattt ctttttttga tgctgcaact
     4141 tctttttcca tatcatcacc atgaccatgc catgtatgag ttcttgtaaa agggagagat
     4201 gccgccatat ctggcgtaga tagcaaatga ccaataccaa cagtaatatt accttcgcta
     4261 tccttataca tggtgttttt gaacccctca tgggctttta aattattaac acacatgctc
     4321 atatagtagc cattatagtt tctggtagat ggtatactaa tatgtgtctt atcagaaata
     4381 ccttcatggc gatttccccc attaccacca gaatgagcac cataaccgct atggctgtcc
     4441 ccatcataac tatgaattgt tccataggga gtattgtgat catgccaccc cggaccttga
     4501 ttagaatttt caataccact catcttaatt tcctctttga catttaaaac aacgatcaat
     4561 acaactgtat atataaccac tggttttata tacagtttaa aattacgccg ctgcgttttc
     4621 cctgtcaacc ctgtggattt tcatttttgt gaaaacggtc
//
LOCUS       DADSZD010000247         4577 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.249, whole genome shotgun sequence.
ACCESSION   DADSZD010000247 DADSZD010000000
VERSION     DADSZD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.249"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            206..1303
                     /gene="mltA"
                     /locus_tag="J1Z28_004223"
     CDS             206..1303
                     /gene="mltA"
                     /locus_tag="J1Z28_004223"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBA7718988.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1380..2186
                     /gene="tcdA"
                     /locus_tag="J1Z28_004224"
     CDS             1380..2186
                     /gene="tcdA"
                     /locus_tag="J1Z28_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBA7718989.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="J1Z28_004225"
     CDS             complement(2237..2680)
                     /gene="csdE"
                     /locus_tag="J1Z28_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBA7718990.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="J1Z28_004226"
     CDS             complement(2680..3885)
                     /gene="csdA"
                     /locus_tag="J1Z28_004226"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBA7718991.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFEGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4077..4304
                     /locus_tag="J1Z28_004227"
     CDS             4077..4304
                     /locus_tag="J1Z28_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBA7718992.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
BASE COUNT         1089 a         1158 c         1235 g         1095 t
ORIGIN      
        1 gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
       61 gattcggcat gattttgaac aaaaacaaat cactaaagcc tgttttttgt gcaaaatttg
      121 cgtcagggag aggctgctca tttcattaaa cgcggtggtt tgttatcttc gttgcgcctt
      181 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      241 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      301 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      361 gccgattaat gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      421 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      481 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgtcgacaa
      541 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
      601 tcagggcgag ttccagtatc cgatttaccg tatgccgcca aaacgtggtc gtctgccgtc
      661 tcgtgcggag atctacgcag gagcgctgag tgataaatat attctcgctt acagtaactc
      721 cctgatggat aacttcatta tggatgtgca gggtagcggg tatatcgact ttggtgatgg
      781 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
      841 taaggtgttg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
      901 tcactggggc gaaacacaca gtgaagctga ggttcgcgag ctgctggaac agaacccgtc
      961 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1021 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1081 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1141 gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg
     1201 gatcgggccg gaagccggac accgcgctgg ttggtacaac cactatggac gtgtctgggt
     1261 gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgata ttctgtatgc
     1321 cgtttttacg gatatgacag ggtgaggggt aacctcaccc tttttaattc tgaggtgcta
     1381 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgactgtatg
     1441 gtgaaaaagc attgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
     1501 tcggttcctg ggcggcggaa gcgctagcgc gcacggggat tggcgcaatc acgcttatcg
     1561 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
     1621 tcgggctggc aaaagcggaa gtcatggcgg agcgtattcg ccagattaac ccggagtgcc
     1681 gtgtaacggt ggtggatgac ttcgtgacgc cggataacgt agcgcaatat atgaatgccg
     1741 gttattcgta cgtgattgat gctattgata gtgtacggcc caaagcggcg ctgattgctt
     1801 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg cagattgacc
     1861 cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgttg gcggcgaagt
     1921 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
     1981 tggattgcgt gttctctacc gaagcgctgg tgtacccgca gtcagacggc accgtctgtg
     2041 cgatgaaagc cacggcggaa gggccaaagc ggatggattg tgcctcagga tttggcgcgg
     2101 cgacgatggt gaccgccact tttggctttg ttgcggtttc tcatgcgctg aagaagatga
     2161 tggcgaaagc ggcgcgtcag gggtaaacaa tcaggtcgga tgcgacgcga acgtcttatc
     2221 cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aatcgcctcg cttaacgcat
     2281 tcaacccctg gctgcgtgag gcgctaagtt gcgcacgtaa tcccagctca tcaaacaatg
     2341 ccagcggcga ctgtgcctgc aattcggcgg cggttttccc ctcaacggca gtcaacaaca
     2401 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaaatgc atcttgccgt
     2461 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
     2521 gcgcttttaa ctcctctggc aaagccggaa gctgttttcc cagcatgatc aactggcgat
     2581 atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct gcggttacgg
     2641 ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca ataattccag
     2701 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
     2761 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgctg
     2821 cccggcgcgc agggcaatac cgtactccgc cagcagtgtc accatatcgc tgtgatgaac
     2881 acccgcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
     2941 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg
     3001 gttgatatcg taatctgtca gccattccag cgccgcgctt aatcctatga cgccagcgac
     3061 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaagc cttcaaaact
     3121 cacttcgtga accattttgc cgccgcccag ccagggcgac atcgcttcca gcagttctgg
     3181 tttgccatac agcacgccga tgcctgtcgg gccatacagt ttgtgacctg aaaaagcata
     3241 gaaatcaata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
     3301 aaccataacc accatcccgg ctgaatgagc aaaagtaatc gctctcgcca gatccgggca
     3361 accgccagtg acgttcgaca tctggcccaa cgccagaatc cgactacggg gagtaatcag
     3421 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
     3481 ggcccccgtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt gttctgccac
     3541 actgacaata atttcatcgc ccggttgcag acgcggacgg gcatagcatt gcgccaccat
     3601 gttgatggat tcagtggtgc cgcgcgtcca gacgatattt ttgtcatccg atgcattcag
     3661 taattgtgcc actttttccc gcgcagcttc gtaacgcgcg gtcaggcgtt gggcttcggc
     3721 aaactggctg cgatggacgt ttccggcgct caggctataa aactgtcggg tggcttcaac
     3781 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
     3841 cgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
     3901 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
     3961 tatctggata tcctggcgaa gccgcgtcag taacatatgc tgaatagtgt taggcgttaa
     4021 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
     4081 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
     4141 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
     4201 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
     4261 tcaaccgtac cgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt
     4321 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
     4381 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
     4441 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg cattccgata
     4501 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
     4561 acggacaatt tataatg
//
LOCUS       DADSZD010000248         4500 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.250, whole genome shotgun sequence.
ACCESSION   DADSZD010000248 DADSZD010000000
VERSION     DADSZD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.250"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            218..1375
                     /gene="acrE"
                     /locus_tag="J1Z28_004228"
     CDS             218..1375
                     /gene="acrE"
                     /locus_tag="J1Z28_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBA7718993.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     SYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADATVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNNKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1387..4491
                     /gene="acrF"
                     /locus_tag="J1Z28_004229"
     CDS             1387..4491
                     /gene="acrF"
                     /locus_tag="J1Z28_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HBA7718994.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIQEVVKTLFEAIILVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPREATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYTLIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERSGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
BASE COUNT         1098 a         1090 c         1179 g         1133 t
ORIGIN      
        1 agattttaat tctacttctt taaatccgcg tcaaaacaaa acagtagaat attaatcttt
       61 ttttgtgttt atgtgccttg agttgccagt attcataact attccttaca tcgacgaatg
      121 ataatttgta ggatagcgaa ctgtattttt ctttctgcga gttaacgcat tgcctttttg
      181 ggcaaataac gcgcttttgg ttttttgagg aatagtaatg acgaaacatg ccaggttttt
      241 cctcctgccc tcctttattc tgatctccgc ggctttaatc gccggttgta acgataaggg
      301 agaagagaaa gctcacgtcg gtgaaccgca ggttaccgtt catattgtga aaacggcccc
      361 gttagaagtt aagactgaat taccaggccg caccaatgct taccgtatag ccgaagttcg
      421 cccacaggtt agcgggatcg tactgaatcg caatttcact gaaggcagcg atgtgcaagc
      481 aggccagtcc ctgtaccaga tcgatcccgc gacctatcag gcaagttatg acagcgcgaa
      541 aggcgaacta gcgaaaagtg aagccgccgc cgccatcgcg catttgacgg taaaacgtta
      601 cgttccgctc gtgggtacga aatacatcag tcagcaggag tacgaccagg ccattgctga
      661 tgctcgccag gccgatgcca ccgtgattgc cgcaaaagcc acagttgaaa gcgctcgcat
      721 caatctcgct tataccaaag tgactgcgcc tattagcgga cgtatcggca aatcgactgt
      781 gaccgaaggc gctcttgtca ctaatgggca aacgactgaa ctggcgactg tccagcagct
      841 cgatcctatc tacgttgatg tgacccaatc cagcaacgat tttatgaggc tgaagcaatc
      901 cgtagagcaa ggaaatttgc ataaggaaaa cgccaccagc aacgtagagt tggtcatgga
      961 gaacggtcaa acctatcccc tgaaaggtac gctgcaattc tccgatgtga ccgttgatga
     1021 aagcaccggc tccataaccc tacgtgctgt cttccctaac ccgcaacata cgcttttgcc
     1081 gggtatgttt gtgcgcgcac ggattgatga aggcgtccaa cctgacgcca ttcttatccc
     1141 gcaacaaggc gttagccgca caccacgtgg tgatgcaacc gtgctgattg ttaacaataa
     1201 aagtcaggtt gaagcgcgcc ctgtcgttgc cagtcaggcg attggcgata aatggttgat
     1261 tagcgaaggt ctgaaatctg gcgatcaagt cattgtcagc ggcctgcaaa aagcgcgtcc
     1321 gggagagcag gttaaagcca ctaccgatac ccccgcagat actgcatcga agtaaggcaa
     1381 tctgacatgg caaacttttt tattcgacga ccgatatttg catgggtgct ggccattatt
     1441 ctgatgatgg cgggcgcact ggcgatccta caattgcccg tcgcccagta tccaaccatt
     1501 gcgccgcctg cggtttctgt ttcggcaaac tatccgggtg ctgatgcgca gaccgtgcag
     1561 gatacagtga cgcaggttat cgaacagaat atgaacggta tcgataacct gatgtatatg
     1621 tcctccacca gcgattccgc cggtagcgtg acaattaccc ttaccttcca gtccgggacc
     1681 gatcctgata tcgcgcaagt acaggtgcag aacaaactcc agctcgccac gccgttgctg
     1741 ccgcaggagg ttcagcagca ggggatcagt gttgaaaagt ccagtagcag ctatttgatg
     1801 gtggcgggct ttgtctctga taaccctggc accacacagg acgatatctc ggactatgtg
     1861 gcctctaacg ttaaagatac gcttagccgt ctgaatggcg tcggtgacgt acagcttttc
     1921 ggcgcacagt atgcgatgcg tatctggctg gacgccgatc tgctaaacaa atataaactg
     1981 acaccggttg atgtgattaa ccagttgaag gtacagaacg atcagatcgc tgccggacag
     2041 ttgggcggaa cgccagcgtt accagggcaa cagttgaacg cctcgattat tgctcagacg
     2101 cggttaaaaa atccggaaga attcggcaaa gtgaccctgc gcgtaaacag tgacggctcg
     2161 gtggtgcgtc tgaaagatgt cgcacgggtt gaacttggcg gtgaaaacta taacgttatc
     2221 gctcgcatca acggaaaacc ggcggcgggc ctggggatta agctggcaac cggcgcgaat
     2281 gctctcgata ccgcgaaagc cattaaggca aaactggcgg aattacagcc attcttcccg
     2341 cagggaatga aggttcttta cccttatgac accacgcctt tcgtccagct ttctatccag
     2401 gaagtggtga aaacgctgtt cgaagccatt atcctggtat tcttggtgat gtatctgttc
     2461 ttgcagaaca tgcgggcaac attgataccc accattgctg tacctgtcgt gttgttaggg
     2521 acatttgcca tactcgccgc ttttggttac tccatcaata cgctaacgat gtttgggatg
     2581 gtacttgcca tcggacttct tgttgatgat gcgatagtcg ttgtggagaa cgtcgagcgc
     2641 gtaatgatgg aggataaact cccccccaga gaagcgacgg aaaaatcgat gtcgcaaatc
     2701 cagggcgcgc tggtaggtat cgcgatggta ctgtcggcgg tatttattcc gatggcattc
     2761 ttcggtggct caacaggggc aatttatcgc cagttctcta tcactattgt ttcagcaatg
     2821 gcgctttcag tactggttgc gctgattctc acccccgcgt tatgtgcaac attgctgaaa
     2881 ccagtctctg ccgagcatca cgaaaataag ggaggattct tcggctggtt taacaccact
     2941 ttcgatcata gcgttaatca ctacaccaat agcgttggca aaatcctcgg ttccacaggg
     3001 cgatatttac tgatctatac gctgattgtt gcaggaatgg tggtgttgtt tttacgtctt
     3061 ccgtcttcct tcttacctga agaggatcag ggtgtctttc tgaccatgat tcagttaccc
     3121 gctggcgcga cgcaagagcg gacgcaaaaa gtgttggatc aagtgacgga ttactatctg
     3181 aagaacgaga aagcgaacgt tgaaagtgtc tttacagtta acggctttag cttcagcggc
     3241 caggcacaaa acgccggtat ggccttcgtc agtctgaaac cgtgggaaga gcgtagtggt
     3301 gacgaaaaca gtgcggaagc agtaatccat cgtgccaaaa tggaattggg caagatccgc
     3361 gacggttttg tcattccatt taatatgcca gccattgttg aactgggcac ggcaacgggt
     3421 ttcgactttg agttaattga tcaggctggg ctgggtcacg atgccctgac ccaggcccgt
     3481 aaccagttgc ttggcatggc ggcgcaacat cctgccagct tagtcagcgt gcgccctaat
     3541 ggcctggaag acaccgcgca gtttaaactg gaagttgacc aggaaaaggc gcaggcatta
     3601 ggtgtttcac tttctgacat caatcagacc atttcaacgg cgttgggtgg gacttacgtt
     3661 aacgacttca tcgaccgtgg tcgcgtgaaa aaggtgtatg ttcaggcgga tgccaaattc
     3721 cgtatgctgc cagaagatgt cgataaactt tatgtccgca gcgccaacgg cgaaatggtg
     3781 ccattctctg cctttaccac ttcacattgg gtgtatggct ctccgcgact ggaacgctac
     3841 aacggtctgc cgtcaatgga gattcagggg gaagccgcgc caggaaccag ttccggcgat
     3901 gctatggcgt tgatggaaaa ccttgcgtca aaattaccgg cgggcattgg ttatgactgg
     3961 acgggtatgt catatcagga acgtttatcg ggaaaccagg ctcccgctct ggtagcaatt
     4021 tcctttgtgg ttgttttcct gtgccttgct gcactctatg aaagctggtc aattcctgtc
     4081 tcggttatgt tggtcgtgcc gttagggatt gtcggcgtgc tactggcggc aacactcttt
     4141 aatcaaaaaa atgacgtcta ctttatggtg ggcttgctaa cgacaattgg cttgtcggcc
     4201 aaaaacgcta ttttgattgt tgagttcgct aaagatctca tggagaaaga gggtaaaggt
     4261 gttgttgaag cgacactaat ggcagtacgt atgcgtctgc gtcctatcct gatgacctct
     4321 ctcgccttta ttctcggcgt attaccgctg gctatcagta acggggccgg cagtggcgcg
     4381 caaaacgcag tcggtatcgg ggtaatgggc ggaatggtct ctgcaacgtt gctggcaatc
     4441 ttcttcgtgc cagtgttctt tgtggtgatc cgtcgatgct ttaaaggata aatcagaaac
//
LOCUS       DADSZD010000249         4477 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.251, whole genome shotgun sequence.
ACCESSION   DADSZD010000249 DADSZD010000000
VERSION     DADSZD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.251"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..234)
                     /gene="chbB"
                     /locus_tag="J1Z28_004230"
     CDS             complement(<1..234)
                     /gene="chbB"
                     /locus_tag="J1Z28_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBA7718995.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEV"
     gene            complement(533..871)
                     /gene="osmE"
                     /locus_tag="J1Z28_004231"
     CDS             complement(533..871)
                     /gene="osmE"
                     /locus_tag="J1Z28_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBA7718996.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1073..1900
                     /gene="nadE"
                     /locus_tag="J1Z28_004232"
     CDS             1073..1900
                     /gene="nadE"
                     /locus_tag="J1Z28_004232"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBA7718997.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2130..3017
                     /gene="cho"
                     /locus_tag="J1Z28_004233"
     CDS             2130..3017
                     /gene="cho"
                     /locus_tag="J1Z28_004233"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBA7718998.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLHEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(2977..3552)
                     /gene="ves"
                     /locus_tag="J1Z28_004234"
     CDS             complement(2977..3552)
                     /gene="ves"
                     /locus_tag="J1Z28_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBA7718999.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3755..4240)
                     /gene="spy"
                     /locus_tag="J1Z28_004235"
     CDS             complement(3755..4240)
                     /gene="spy"
                     /locus_tag="J1Z28_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBA7719000.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
BASE COUNT         1121 a         1089 c         1073 g         1194 t
ORIGIN      
        1 tacttcaacc ggtttgttgg gtaacaaacg ctggatttcg ggcaacatat aagcaatctg
       61 cggccctaat aacacgacat cggcattctg acctttttca ccagccagtg tttccggaaa
      121 tgcttcaata atgaccggaa cttcatattt ttctgcctgt gcgcgcattt ttgataccag
      181 taaagaggta gacatgcccg cagaacaaaa cagataaatg tgtttctttt ccataaaact
      241 gccctcatcg acgattatct gtcagccaga cactccgcaa gccttaacct gcttccatgc
      301 tctgggtaac ttgcgaaacc aacatgactt tttttgtggc tgaaaggagt atacggtata
      361 ggcctgagca atagtatttc tttgacctga taaattgtct ctccttgacc tgtggttatg
      421 accctcttca catttcgggc aaataattcg cgaagataat taagaacaca ggccaaaaaa
      481 aaccggcgca atggccggtt tccgttgtta ctcaagcttt caggcgaggt gattacttcg
      541 tagcctgtgg atcagtgtcg tattcagcac aggtctgata accggagttg atgacatgtc
      601 cggtatcatc taacgcgaca aagtaggttt ctgctttacc atcacgttga cccaggatgt
      661 aggtctggca agtaccacga gcatggatca tgctcacttc agacgaaggt ttacccgcaa
      721 tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc tttcaccaca ggctgtacaa
      781 actggtcttt ggtacgatca taagccgtac aacccgccag catggttaat accgccgctg
      841 cactcagaat tcctgccata ttcttgttca tattccgtcc tcttgtttat cagcgtgtta
      901 gataagcctg gaatacattg ggcgcttttt caagcccgtg aacgaaacgg ctccgctttc
      961 agaggattcc tgtatgacgt tttaaccacc attcagcccg ctgtcgcttg tcgtttcagt
     1021 agcaacgggt tagctttaag gaagttttgt cttttctgtc tggaggggtt caatgacatt
     1081 gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag attaatgctg aagaggaaat
     1141 tcgtcgtagt atcgattttc tgaaaagcta cctgcaaact tatccgttca ttaaatcact
     1201 ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc ggtaagctgt gccagatggc
     1261 gattaatgag ctgcgccagg aaaccggcaa cgaatcgctg caatttattg ccgtacgcct
     1321 gccctatggt gttcaggccg acgaacaaga ttgccaggat gccatcgcct ttattcaacc
     1381 ggatcgcgta ttaaccgtta atatcaaggg cgcggtactc gccagcgaac aggcattgcg
     1441 ggaagcaggc attgaactga gcgattttgt ccgtggcaat gaaaaagcgc gtgagcggat
     1501 gaaagcacaa tatagcattg cgggtatgac cagcggtgtc gtggtaggca ccgatcatgc
     1561 agcagaagcc attaccggat tcttcactaa atatggtgac ggcggtacgg acattaatcc
     1621 gctgtatcgt ctcaacaaac gtcagggtaa gcagttactg gcggcattag gttgcccgga
     1681 acacctttat aagaaagcgc caacggccga tcttgaagat gatcgccctt ctctgccgga
     1741 tgaagtggca ctcggcgtga cctatgacaa tatcgacgac tatctggaag ggaaaaacgt
     1801 acctgaacag gtcgccagaa caatagagaa ctggtatctg aaaaccgaac ataaacgccg
     1861 tccgccaatt accgttttcg atgatttctg gaaaaagtaa taatttgcac atattggttt
     1921 gtacgaaaaa gagtactttg ttcacgccgg atgcggcgtg aacgtcttac tcgacctaca
     1981 aaactatgca aattcaatac attgcaggag tcgaataggc ctgacaggcg tagcacgtca
     2041 aacggtgtaa cctttgtcat cgacccgcct cttttttaat cgctctccgc ctgttacact
     2101 ggatagataa ccagcattcg gagtcaacag tggtacggcg tttaacttct ccgcggctcg
     2161 aatttgaagc tgcggcaatt tatgaatatc ccgaacattt acgttcattc cttaatgact
     2221 tacccacccg accaggggtg tatctgtttc atggcgaaag tgacaccatg ccgctctata
     2281 tcggcaaaag cattaacatc cgcagccgcg tcctttctca tttacgtacc ccggatgaag
     2341 ccgccatgct acggcaatcc cgacgcatca gctggatatg taccgccggt gaaatcggcg
     2401 ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc actgtttaat aaacgcttgc
     2461 ggcgcaatcg ccagctctgt gccctgcaat tacatgaaaa gcgcgtcgat gtggtgtatg
     2521 ccaaagaggt ggatttttca cgagccccca acctgtttgg cctgtttgcc aataggcgcg
     2581 cagctttgca agcattgcag accatcgctg atgaacaaaa actttgttat ggcctgttgg
     2641 gactggagcc gttaagtcgc ggtcgtgcat gttttcgttc agcgctaaaa cgttgcgccg
     2701 gagcatgctg cggtaaagag agccatgacg atcatgcgct acgtttgcgc cagtctctgg
     2761 aacgtttacg agtcgtttgt tggccgtgga aaggtgcggt agcgctgaaa gaacagcacc
     2821 cggaaatgac tcaatatcat attattcaaa actggctgtg gctgggggcg gttaattcgc
     2881 tgaaagaagc gacaacgtta attcgggcac ccgccgggtt tgatcacgac ggttataaaa
     2941 ttctttgtaa gccgctgctt tccggtaact atgaaattac tgaacttgat ccggtgaatg
     3001 accagcaagc cagttgattt cactgaacaa cagtttacct tgcggttgca aaagccgcag
     3061 ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta gtgagcagtt tgtcgcccaa
     3121 ttgccacgcc ccgtttatta caaacaccac cccgccgcgt gaaccaaagg tagtaaacgt
     3181 acgttcggca attctgactt tcgccttaca gacatccagg cgtgtcatga tgttgaaatc
     3241 catcgacatc tgccctgccg tcagtttcgc ttttaccacc aggtccgctg caaaggaaaa
     3301 aggttgtaac ggttttaagg tatggttaaa gcggtctgcg ctttcaagga acatctcgcc
     3361 gccttccagc aacgtcacta tcctttccat accgggaaac aaagaaaact ccccattcgc
     3421 cgcgatggat gtaatgctgg cacgccagta aaaatcacgt tttgccggtg ggaaagtgca
     3481 aatttcgcgc gtttcaccgg cagcgtttcg ccacagattc accgacattt tacgcatatc
     3541 aaagtattcc atacccgctc ccggcccgtt cttttcattg ttatcgttca ggtaaccgtg
     3601 gatggttatc ggcaactcgg gtgatttacg ttagtggcga tcaggctttt tttgcatctg
     3661 tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc attactgcaa ggtagtggac
     3721 aagaccggcg gtcttacgtt ttttggctga aagattattc agcagttgca ggcattttac
     3781 cttttgccgc tggacgttct gtcagacgct tctcaaaatt agcattaaat tgctttttct
     3841 gttccggcgt caggatgttg taaatcttgt tctgggtttc catgtgcgcc agcatgttag
     3901 ctttgcgctg ttcttccatt tttgcgatct gcgcttcagc ttttgcttta tcgaaggtat
     3961 cgctggcaat gatgtcatgc attgcgcggc gttcttccag cggcggacgt ttcatctggt
     4021 cacgctggcc tttcatgatt tcgcggatct gctgtttctg cgcgtcggtc aggttcaggt
     4081 ctttgaacat catgtcctca tgcggaccga acttgccttt atggtgcatc atcggcttcg
     4141 cgtcagccgg tgctgcggta gtggtgtctg cggcatgggc caggttagcc gcgccaagag
     4201 ccagggtaga ggcaacaaac agtgcagtta atttacgcat attctatatc cttcctttca
     4261 gttatttatt acggctttct taagtagcgt gccgtgttga cgagattaac tttactgact
     4321 ttagcgtcaa ttaatcagag caacggtaaa acaatgaaag tgtaaaaaac acttttgcgc
     4381 caattatgga gaaaaaaaga aaatttgatg gagagtgatg aaagaatatt acaacacgat
     4441 gattttgcag agattatgaa gaactatacc ggatgac
//
LOCUS       DADSZD010000250         4475 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.252, whole genome shotgun sequence.
ACCESSION   DADSZD010000250 DADSZD010000000
VERSION     DADSZD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.252"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            9..2912
                     /locus_tag="J1Z28_004236"
     CDS             9..2912
                     /locus_tag="J1Z28_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBA7719001.1"
                     /translation="MVQAQGRQLVKKGKDFTTLCPFHDEKTPSCVISPGKNLYHCFGC
                     NAGGSVLDWVMQTEKLSLRKAVERLKAELGDNPAVVPLVTKDEPEIFAEDEAGRQQLL
                     SRVVEFYHHTLLNAPEAVAYLEKRRLNHPELVAAFKLGFANRTLAYRLPSKKVLAGEK
                     IRTRLKATGVLRESGHEHFTGSLVVPVMDLNGQIREMYGRKIERPQRGLPAHLYLPGA
                     HGGVWNEQALIGSSTVVLCESLIDAMSFWVAGFRNVTAAYGVNGFTDEMLAAFRAHGV
                     KQVLIAYDNDPAGNEAAVKLASSLAASGIATFRVLFPAGMDANEYLCKVSEPERAFSL
                     LLDGAVPVADVVGAESIAPQASAEPAHLTSLAAGVATLPAAAAPAPGVSVEALPEGEL
                     MIALPGERWTIRGLSRKPNAAAMKVNAQVLDTESGVVFADAVDMMSARSLGGYARAAA
                     AELGLAEGDLRRSLGKVLLALEHWQAQPEPENTAPEMSDTDRDAALALLRDECLASRI
                     AADMAACGVVGESTNLTAAYLAAVSRKLDRPLAVLIQSSSAAGKSSLMDAVLNLIPPE
                     ERLQYSAMTGQSLFYLGETNLQHKILAIAEEEGVRQAAYALKLLQSDGELTIASTGKD
                     DASGNLVTKQYTVKGPVMLMLTTTAIDVDEELLNRCLVLTVNESREQTEAIHAAQRYA
                     QTLEGLLASNEKSYITQLHQNAQRLLRPLNVVNPFASQLTFMSDKTRTRRDHMKYLTL
                     IQAIALLHQYQREIKRVEHRGQVVEYIEVTREDIALANYLAHEILGRTLDEMPPQTRK
                     LLLLIQTMVGELAAAQHCKPGEVRFTRREIRDFTQWSDNQLKVHCMRLAEMEYLLIHG
                     GSRGHLLQYELLWDGSGDGSHLCGLIEPEDNQVHDSGKLGHDAGKLPLSCPHVGAKLG
                     AVKSPVSRASAGTDVPQVGEDEKTLFPAARKTSHRNSNTPESRPTPHDGAPAV"
     gene            3113..4207
                     /gene="xerC"
                     /locus_tag="J1Z28_004237"
     CDS             3113..4207
                     /gene="xerC"
                     /locus_tag="J1Z28_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerC"
                     /protein_id="HBA7719002.1"
                     /translation="MTRPIPPGPEELYLSRQNLTQRQYVVVWLEYLTQRQYVVVWLEY
                     LTAQNYSAVSVSDYRERVLPFVTWCEERGLMYAPQVSQPQLESYQRHLHGYRKADGHP
                     LAVSGQVGRSSAIKIFYRWLLKRHVILYNPADLLELPKPDKRLPAQVFSEDETRQVLQ
                     SLDVSTPVGLRNRTILEVLWSTGIRRMELANLMLWDVDAGRGVMNVRQGKGRKDRVVP
                     VGHVALAWLARYLKDVRPQMTRQRDSGHLFVTKYGSGLKRNTLTWLAGTAIRETARID
                     KPGACHIFRHSMATQMLDNGADTRHIQAILGHEKLETTQIYTKVAIKPLQKVHARTHP
                     AEKRRTETRGQQPESTEPDGATVSPDRLKS"
BASE COUNT          929 a         1265 c         1512 g          769 t
ORIGIN      
        1 tagtcgccgt ggttcaggcg caggggcgac aactggttaa gaaagggaag gacttcacca
       61 cgctgtgccc gttccatgac gagaaaacgc cgtcctgtgt gatctcaccg gggaaaaacc
      121 tgtatcactg cttcggctgt aacgcgggcg gatcggtgct ggactgggtg atgcagacgg
      181 aaaaactcag cctgcgtaag gcggtggaac gtctgaaagc ggagctgggc gacaatcccg
      241 ccgtggtgcc gctggtcacc aaagatgaac cggaaatctt cgcggaagat gaagcgggcc
      301 gtcagcaact gctctcccgt gtggtggagt tctaccacca cacactgctg aacgcgccgg
      361 aagcggtggc gtatctggaa aagcgccgcc ttaaccatcc tgaactggtc gccgccttta
      421 aactcggctt cgccaaccgc acgctggcct accgtctccc gtccaaaaaa gtgctggcag
      481 gcgagaagat ccgcacccgg cttaaggcga cgggtgtgct gcgcgagtcc gggcatgagc
      541 acttcaccgg gtcgctggtt gtgccggtga tggatctgaa cgggcagatc cgcgagatgt
      601 acgggcgcaa gatcgagcgt ccacaacgtg gcctgcccgc acacctgtat cttcccggcg
      661 ctcacggcgg cgtgtggaat gaacaggcgc tgatcgggtc atcaacggtt gtcctgtgcg
      721 aatcgctgat cgatgccatg tcgttctggg tggccgggtt ccgcaatgtc acggcggctt
      781 acggggtgaa cggcttcacc gatgagatgc tggcggcgtt ccgggcacac ggcgttaagc
      841 aggtgctgat cgcctacgac aacgatccgg cgggcaatga ggcagcggtg aaactggctt
      901 cttccttagc cgcttccggg atcgccacgt ttcgggtact gttcccggcg ggcatggatg
      961 ctaacgaata tctgtgtaag gtgtcggagc cggaacgggc gttcagtctg ttgctggatg
     1021 gcgcggttcc ggtggctgac gtcgtgggtg cggagagtat cgcgccgcag gccagcgcgg
     1081 agcctgcaca cctgacttca ttagctgccg gggttgcgac cttacccgca gccgccgcgc
     1141 ctgcaccggg cgtcagcgtg gaagcgttgc cggaaggtga actgatgatc gccttaccgg
     1201 gagaacgctg gacgatacgg ggcttaagcc gcaaaccgaa cgcggcggcg atgaaggtta
     1261 acgcgcaggt gctggacacg gaaagcggcg tggtgttcgc ggatgcggtg gacatgatga
     1321 gcgcccgcag tctcggcggt tatgcgcggg ccgcagcggc agagctgggc cttgcagaag
     1381 gcgatctgcg ccgctcgtta ggaaaagtgt tgctggcgct ggagcactgg caggcgcagc
     1441 cagagccgga gaacaccgcc ccggaaatga gcgacacgga tcgggacgcg gcgctggcgc
     1501 tgctgcgcga cgagtgtctt gcgtcacgca tcgcggcaga catggcggcg tgtggcgtgg
     1561 tgggtgagtc caccaacctg acggcggcgt atctggcggc ggtgtcgcgt aagctggaca
     1621 ggccgctggc ggtgctcatc cagtcatcga gcgcggcggg caagtcctcg ctgatggatg
     1681 cggtgctgaa cctgatccca ccagaggagc gcctgcaata cagcgccatg accgggcaga
     1741 gcctgttcta cctcggcgaa accaacctgc aacacaagat cctcgccatc gcggaagaag
     1801 aaggtgtgcg ccaggcggcg tatgcgctca agctgttaca gtcggacggg gaactgacca
     1861 tcgccagtac gggcaaggac gatgccagcg gcaacctggt cacgaagcag tacacggtga
     1921 aaggcccggt gatgctgatg ctgaccacca cggcgatcga cgttgacgag gaactgttaa
     1981 accgttgcct ggtgctgacg gtgaacgaga gccgggaaca gacggaggcc atccacgcgg
     2041 cgcagcgtta tgcgcagacg ctggaggggc tgctggcgtc gaatgaaaaa agttacatca
     2101 ctcagcttca ccagaacgcg cagcggctgc tgcgcccgct caacgtggtg aacccgttcg
     2161 cgtcacagtt gaccttcatg agcgacaaga cgcgcacccg gcgcgaccat atgaaatacc
     2221 tgacgctgat ccaggcgatc gcgctgttgc accagtacca gcgggagatc aaacgcgtgg
     2281 agcatcgcgg gcaggtggta gagtacatcg aggtgacgcg ggaggatatc gcgctggcca
     2341 actatctggc acatgaaata ctcgggcgca cgctggatga gatgccgccg cagaccagaa
     2401 agctgttgct gctgatccag acaatggtcg gtgaactggc agcggcgcag cactgcaaac
     2461 ccggcgaggt acgcttcacg cgccgggaga tccgcgactt cacacagtgg agcgacaacc
     2521 agttgaaggt ccactgcatg cgccttgcgg aaatggaata cctgttgatc cacggcggca
     2581 gccggggtca cctgctacaa tacgagttgc tgtgggacgg cagcggcgac ggctcgcacc
     2641 tgtgcggcct gatcgaaccg gaggataatc aggtgcatga ctccggcaag ttggggcatg
     2701 atgcaggcaa gttgccctta agttgccccc atgttggggc taagttggga gcggtgaaat
     2761 cgcctgtaag ccgcgccagt gctggtacgg atgtgccgca agttggggaa gatgaaaaaa
     2821 cactgttccc ggcagccagg aaaacatcac atcgtaacag taatacgccg gaaagccgcc
     2881 cgacacccca tgacggtgcg ccagcggtat gactcccgca agttggatct gaatcatcgc
     2941 aagttaccct taagttgccc tcatgttagg gctaagttgg gggcggtgaa atcccccaca
     3001 gaccgcgcca tttctggcct gaacgccccg caagttgggg cagcacaaaa accactgttc
     3061 catcctctgt tcttatccct gtcgtaccct aatctcaatc aggacaacca tcatgaccag
     3121 acctatccct ccggggccgg aggagcttta tcttagccgc cagaacctga cgcagcgcca
     3181 gtatgtggtg gtgtggctgg aatacctgac gcagcgccag tatgtggtgg tgtggctgga
     3241 atacctgacg gcacagaact acagcgcggt cagcgtaagc gactaccgcg agcgggtgtt
     3301 accgttcgtg acgtggtgcg aggagcgcgg gctgatgtac gcgccgcagg tcagccagcc
     3361 acagcttgaa agttatcagc gccaccttca cggctaccgt aaggcggacg gccatccgct
     3421 ggcggtcagt ggtcaggtgg gccggagcag cgcgataaaa atcttttacc gctggctgct
     3481 gaagcggcat gtgatcctgt acaaccccgc cgatctgctg gaactgccaa agccggacaa
     3541 acggcttccg gcgcaggtgt tcagtgagga cgagacgcgg caggtgttgc agagtctgga
     3601 tgtgagcact cccgttggcc tgcgtaaccg gacgatcctt gaggtgctgt ggagtaccgg
     3661 gatcaggcgg atggagctgg ctaacctgat gctgtgggac gtggacgccg ggcgtggcgt
     3721 gatgaacgtg cgccagggta aggggcgtaa ggatcgggtg gtgccggtgg gccatgtggc
     3781 gctggcgtgg ctggcgcggt atctgaaaga cgtgcggcca cagatgacgc gccagcggga
     3841 cagcggtcat ctgttcgtga cgaaatacgg cagcggcctg aaacgaaata cgctgacgtg
     3901 gctggcaggc accgcgatcc gggaaacggc gcggatcgac aaaccgggcg cgtgccatat
     3961 cttccggcac tcgatggcga cgcagatgct ggacaatggc gcagacacac ggcacatcca
     4021 ggcgatcctc ggccatgaga agctggagac gacgcagatc tacacgaagg tggcaataaa
     4081 gccgttgcag aaggttcatg cgcggacgca cccggcagag aagcggcgaa cggaaacgcg
     4141 ggggcagcag ccggagagca cagagccgga cggagccacc gtgtcgccgg acaggctgaa
     4201 aagttaggtg gcgatccctg agaggccgtg gagtgaggtg cagggcagac aaccgccttc
     4261 cctggcggtt gactgccccc ctgccagcgg agtgcgcggg ggtaaatgcc gcccgccccg
     4321 tggaggctgc cgcgccccat gcggccctgc ggcacggggc aaacgtcatt caacataatg
     4381 cgggggatta tgcgcactct cgccgggtgg caatgatggc gaacccgatc cgctgcgcgc
     4441 ggctcgtgtt cgcacaatcc gcattatgtc ctgcg
//
LOCUS       DADSZD010000251         4458 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.253, whole genome shotgun sequence.
ACCESSION   DADSZD010000251 DADSZD010000000
VERSION     DADSZD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.253"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(39..1541)
                     /gene="araA"
                     /locus_tag="J1Z28_004238"
     CDS             complement(39..1541)
                     /gene="araA"
                     /locus_tag="J1Z28_004238"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBA7719003.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            complement(1552..3252)
                     /gene="araB"
                     /locus_tag="J1Z28_004239"
     CDS             complement(1552..3252)
                     /gene="araB"
                     /locus_tag="J1Z28_004239"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBA7719004.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHTPGNVDYSRYIGGIYSSEWFWAKI
                     LHITRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTETWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTAQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            3591..4436
                     /gene="araC"
                     /locus_tag="J1Z28_004240"
     CDS             3591..4436
                     /gene="araC"
                     /locus_tag="J1Z28_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBA7719005.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
BASE COUNT          978 a         1194 c         1261 g         1025 t
ORIGIN      
        1 cagcgtcgca tcaggcgtta catacctgat gcggctactt agcgacgaaa cccgtaatac
       61 acttcgttcc agcgcagcgc gtctttaaac gctggcaggc gggtgtcgtt atcaatcacc
      121 gtgatttcaa tgtcgtgcat ctcggcaaac tggcgcatat cgttgaggtt cagcgcatgg
      181 ctgaagacgg tatggtgcgc gccaccagca aggatccacg cttcggaagc ggttggtaga
      241 tccggttgcg ctttccacag cgcattcgcc accggcagtt tcggcaggga gtgcggtgtt
      301 ttcaccgtgt cgatgcagtt aaccagcaga cggtaacgat cgccgagatc aatcaggctg
      361 gcgacaatgg ctggaccggt ttgagtgttg aagatcaggc gggcaggatc gtctttaccg
      421 ccaataccga gatgctgaac gtcgaggatc ggtttctctt ccacagcgat cgacggacag
      481 acttccagca tatgggagcc gagtaccagg tcattacctt tctcgaagtg ataggtgtag
      541 tcctccataa aggaggtgcc gccctgcaga ccggttgaca tcaccttcat gatgcgaagc
      601 agggcggcag ttttccagtc gccttcgccc gcaaagccgt agccctgctg catcagacgc
      661 tgtacggcca gacccggaag ctgtttcagg ccgtgcaaat cttcaaaggt agtagtgaaa
      721 gcgtggaagc caccttgttc caggaaacgc ttcatcccca gctcaatacg cgccgcttcc
      781 agcacgttct gtcgtttttc gccgtggatt tgtgtggcag gtgtcatggt gtagcagctt
      841 tcgtactcat cgaccagcgc gttaacatcg ccgtcgctga tggagttcac cacctgcacc
      901 agatcgccaa ccgcccaggt attgacggag aaaccgaact tgatctgtgc ggcaacttta
      961 tcgccatcgg tgaccgctac ttcacgcatg ttatcgccaa aacggcagac tttcagatga
     1021 cgggtatcct gtttagagac cgcctgacgc atccaggagc cgatacgctc atgtgcttgt
     1081 ttatcctgcc agtgaccggt aacgacggca tgttgttgac gcatacgcgc gccgatgaag
     1141 ccgaactcgc gaccgccatg cgcggtctgg ttcaggttca taaagtccat gtcgatgctg
     1201 tcccacggca gcgccgcgtt gaactgggtg tggaattgca gcaacggttt gttgagcatg
     1261 gtcaggccgt tgatccacat tttggccggg gagaaagtgt gcagccacac taccagcccg
     1321 gcgcaacgat cgtcgtaatt cgcgtcgcgg caaatagcgg tgatttcatc cggcgtggtg
     1381 cccagcggtt tcagcaccag tttgcagggc aacttcgctt ctgtattcag tgcattaaca
     1441 acgtgttccg catgttgcgt cacctggcgc agagtctccg ggccgtaaag atgctggctg
     1501 ccaattacaa accacacttc ataattatca aaaatcgtca ttgtcgtgtc cttatagagt
     1561 cggaacggcc tgggcagcct gtgccggggc ggaagttgga agatagtgtt gttcggcgct
     1621 catcgcccat tgctgatagc ggcgataaag ctgttcaaag cgttgtgcct gttcgctgca
     1681 cggttgcagg gttttctcta ccgcactggc cattttttgc tgggctgagg ggatatcggc
     1741 gtgcactttt gcggcgacgg cagcgaaaat cgccgcaccg agcgcgcaac attggtcaga
     1801 agcaacaatt tgcagcgggc gattcagcac gtcgcagcag gcctgcataa tgacctggtt
     1861 tttgcgcgcg atgccgccca gtgccatcac gttattgacg gcgatcccct gagcggtgaa
     1921 gcactccata attgcgcgtg cgccaaaggc ggtggcggca atcaaaccgc cgaacagcag
     1981 cggagcgtcg gtagcgaggt tcagatcggt aatcacccct ttcaggcgtt ggttagcgtt
     2041 cggtgtgcgg cggccgttaa accagtcgag caccaccggc aggtgatcca gagacggatt
     2101 tttggcccat gcttcggtca gcgccggaag cagttgtttc tggctggcgt tgatttgctc
     2161 tttcagttcc ggatgctggg cggcaagctg ttccagcggc cagccgagta cgcgaccgaa
     2221 ccaggcgtag atatccccaa acgccgattg gcctgcttcc agaccgataa atccaggcac
     2281 cacgctgcca tcaacctgac cgcaaatacc tttcactgcc cgctcgccaa cgctctgttt
     2341 gtcggcaatc agaatgtcgc aggtggaagt accgataact tttaccagtg cgttaggctg
     2401 tgcgcctgcg ccaactgcgc ccatatggca gtcaaacgcg ccgccggaaa tcaccacgct
     2461 ttcaggcagg ccgagacgct gcgcccattc cgggcataag gtgcccaccg gaatatcggc
     2521 agtccaggtt tcagtgaaca gcggggaagg taaatgacgg ttgaggatcg ggtccagctc
     2581 atcaaagaaa ctggctggcg gcaggccacc ccagctttcg tgccatagag atttatgccc
     2641 ggcgctgcaa cgtccgcgac gaatatcctg cgggcgggtg gtaccggaaa gcagagctgg
     2701 cacccagtcg cacagctcaa tccacgatgc ggcagattgt gccacggcgt tgtcctggcg
     2761 agttatatgc aggatttttg cccagaacca ttcgctggaa taaataccgc caatatagcg
     2821 ggagtagtca acgttgcccg gcgtgtgaca taagcgggta atctcttccg cttcttcaac
     2881 cgcggtgtgg tctttccaca atacgaacat cgcgttcggg ttttcggcaa actccgggcg
     2941 cagcgccagc acgttaccgt cggcgtcaat cggtgcgggc gtcgagccgg ttgtgtcaac
     3001 gccaatcccg accacagctg cgcgctgttc gacgctaagc tctgcaagca cggttttcag
     3061 cgccgcttcc attgactcaa tgtagtcacg cggatgatga cggaactggt tattcggggc
     3121 atcacaaaat tgcccgttct gccaacgggg ataccactct acgctggtgg cgatctcttc
     3181 accggtggcg cagtccaccg ccaaagctcg cacagaatca ctgccaaaat cgaggccaat
     3241 tgcaatcgcc atcgtttcac tccatccaaa aaaacgggta tggagaaaca gtagagagtt
     3301 gcgaaaaaaa acgtcaggct ggatccgcta atcttatgga taaaaatgct atggcatagc
     3361 aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct gccgtgatta tagacacttt
     3421 tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta cagaatgctt ttaataagcg
     3481 gggttaccgg ttgggttagc gagaagagcc agtaaaagac gcagtgacgg caatgtctga
     3541 tgcaatatgg acaattggtt tcttctctga atggcgggag tatgaaaagt atggctgaag
     3601 cgcaaaatga tcccctgctg ccgggatact cgtttaacgc ccatctggtg gcgggtttaa
     3661 cgccgattga ggccaacggt tatctcgatt tttttatcga ccgaccgctg ggaatgaaag
     3721 gttatattct caatctcacc attcgcggtc agggggtggt gaaaaatcag ggacgagaat
     3781 ttgtctgccg accgggtgat attttgctgt tcccgcctgg agagattcat cactacggtc
     3841 gtcatccgga ggctcacgaa tggtatcacc agtgggttta ctttcgtccg cgcgcctact
     3901 ggcatgaatg gcttaactgg ccgtcgatat ttgccaatac cggtttcttt cgcccggatg
     3961 aagcgcacca gccgcatttc agcgacctgt ttgggcaaat cattaatgcc ggacaagggg
     4021 aagggcgcta ttcggaattg ctggcgataa atctgcttga gcaattgtta ctgcggcgca
     4081 tggaagcgat taacgagtcg cttcatccgc cgatggataa tcgggtacgc gaggcttgtc
     4141 agtacatcag cgatcacctg gcagacagta attttgatat cgccagcgtc gcacagcatg
     4201 tctgcctgtc gccgtcgcgt ctgtcgcatt tgttccgcca gcagttaggg attagcgtat
     4261 taagctggcg cgaggaccag cgcatcagtc aggcgaagct gcttttgagc accacccgga
     4321 tgcctatcgc caccgtcggt cgcaatgtcg gttttgacga tcaactctat ttctcgcgag
     4381 tatttaaaaa atgcaccggg gcgagtccga gcgagtttag ggcaggttgt gaataataag
     4441 aaagaagggg gggaaccc
//
LOCUS       DADSZD010000252         4455 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.254, whole genome shotgun sequence.
ACCESSION   DADSZD010000252 DADSZD010000000
VERSION     DADSZD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.254"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            3..569
                     /gene="slp"
                     /locus_tag="J1Z28_004241"
     CDS             3..569
                     /gene="slp"
                     /locus_tag="J1Z28_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBA7719006.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            722..1252
                     /locus_tag="J1Z28_004242"
     CDS             722..1252
                     /locus_tag="J1Z28_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7719007.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTIAIIDFVALI
                     NGSYCSVPEADVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHINYLV"
     gene            complement(1309..2337)
                     /gene="chuS"
                     /locus_tag="J1Z28_004243"
     CDS             complement(1309..2337)
                     /gene="chuS"
                     /locus_tag="J1Z28_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hematinate-forming heme oxygenase ChuS"
                     /protein_id="HBA7719008.1"
                     /translation="MNHYTRWLELKEQNPGKYARDIAGLMNISEAELAFARVTHDAWR
                     MRGDIREILAALESVGETKCICRNEYAVHEQVGAFTNQHLNGHAGLILNPRALDLRLF
                     LNQWASVFHIKENTARGERQSIQFFDHQGDALLKVYATDNTDMAAWSELLARFITDEN
                     TPLELKAVDAPVVQTRADASVVEQEWRAMTDVHQFFTLLKRHNLTRQQAFNLVADDLA
                     CKVSNSALAQILESAQQDGNEIMVFVGNRGCVQIFTGVVEKVVPMKGWLNIFNPTFTL
                     HLLEESIAEAWVTRKPTSDGHVTSLELFAHDGTQIAQLYGQRTEGEQEQAQWRKQIAS
                     LIPEGVTA"
     gene            complement(2386..4368)
                     /locus_tag="J1Z28_004244"
     CDS             complement(2386..4368)
                     /locus_tag="J1Z28_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HBA7719009.1"
                     /translation="MSRPQFTSLRLSLLALAVSATLPTFAFATETMTVTATGNARSSF
                     EAPMMVSVIDTSAPENQTATSATDLLRHVPGITLDGTGRTNGQDVNMRGYDHRGVLVL
                     VDGVRQGTDTGHLNGTFLDPALIKRVEIVRGPSALLYGSGALGGVISYDTVDAKDLLQ
                     EGQSSGFRVFGTGGTGDHSLGLGASAFGRTENLDGIVAWSSRDRGDLRQSNGETAPND
                     ESINNMLAKGTWQIDSAQSLSGLVRYYNNDAREPKNPQTVEASDSSNPMVDRSTIQRD
                     AQLSYKLAPQGNDWLNADAKIYWSEVRINAQNTGSSGEYREQITKGARLENRSTLFAD
                     SFASHLLTYGGEYYRQEQHPGGATTGFPQAKIDFSSGWLQDEITLRDLPITLLGGTRY
                     DSYRGSSDGYKDVDADKWSSRAGMTINPTNWLMLFGSYAQAFRAPTMGEMYNDSKHFS
                     IGRFYTNYWVPNPNLRPETNETQEYGFGLRFDDLMLSNDALEFKASYFDTKAKDYIST
                     TVDFAAATTMSYNVPNAKIWGWDVMTKYTTDLFSLDVAYNRTRGKDTDTGEYISSINP
                     DTVTSTLNIPIAHSGFSVGWVGTFADRSTHISSSYSKQPGYGVNDFYVSYQGQQALKG
                     MTTTLVLGNAFDKEYWSPQGIPQDGRNGKIFVSYQW"
BASE COUNT         1161 a         1106 c         1018 g         1170 t
ORIGIN      
        1 acatgaacat gacaaaaggt gcactcatcc tcagcctttc atttttgctt gccgcatgta
       61 gttcaattcc gcaaaatatc aaaggcaata accaacctga tattcaaaaa agttttgttg
      121 ctgttcataa ccagccgggg ttatatgttg gtcaacaagc gcgctttggt gggaaggtta
      181 tcaacgttat caatggcaaa acggatacgt tgttagaaat cgctgtatta ccgttggata
      241 gctatgcgaa gcctgatatt gaagccaact atcagggccg actgctcgcc agacaaagcg
      301 gcttccttga tccagtgaac tatcgtaatc actttgttac catcctcggt accattcagg
      361 gtgaacaacc tggctttatc aataaagtcc cgtataactt cctggaagtg aatatgcagg
      421 gcatccaggt gtggcatttg agagaagtgg ttaataccac ctataacttg tgggattacg
      481 gctatggtgc attctggccg gaaccgggct ggggggctcc ttactacacc aatgcggtga
      541 gtcaggtaac acctgagctg gtcaaataac accaccgaaa gatgcagatg ctttctcagc
      601 atctgcatca tgcattacat caaattaata cacagtaagc taactattat tataagccct
      661 gtcctgttaa ttacctttgg caaactgatt ataaagttaa tgtccgtacc aggagtcggt
      721 tatgtttctt ataattacca gggatacgat gtttttcacc gcgatgaaaa acattctgag
      781 taaaggtaat gtcgttcata tacagaacga agaagagatc gacgtaatgt tgcatcagaa
      841 tgccttcgtc attattgata cattaatgaa taatgtattt cattctaatt ttctcactca
      901 aattgaacga ttaaaacctg tccatgtaat tattttctcc ccctttaata ttaaacgctg
      961 cctggggaaa gtaccggtga cctttgttcc gcgaactatc gccatcatcg actttgtcgc
     1021 actcatcaat ggcagttact gctctgtgcc tgaagcggat gtgtccctct cgcgcaagca
     1081 gcatcaggtt ctgagctgta ttgcgaatca aatgacaacg gaagatattc tggagaaact
     1141 gaaaatatcg ctaaaaacgt tctactgcca taaacacaat atcatgatga tcctcaatct
     1201 taagcggatc aatgagctgg tacgccatca gcatattaat tatctggtgt gaacgattga
     1261 acaatataaa gaggcccagc aacagccaga cctcccgtta attatacgtt atgcagtaac
     1321 gccttccggt atcaacgaag caatttgctt acgccattgc gcttgctcct gttcaccttc
     1381 tgtacgttga ccataaagtt gcgctatctg cgtaccatca tgggcaaaca attccaggct
     1441 ggttacgtgg ccatcgctgg tcggtttacg ggtaacccag gcttcagcaa tgctctcttc
     1501 taatagatga agggtaaacg tcgggttgaa aatattcagc caacctttca ttggcaccac
     1561 tttttctacc acaccggtga aaatctgtac gcagccacgg ttgccaacaa acaccatgat
     1621 ttcattgcca tcctgctgtg cagattcaag aatttgcgcc aacgcactgt tggatacttt
     1681 gcaggccaaa tcgtctgcca ccagattgaa cgcctgttgg cgcgtcaggt tgtggcgctt
     1741 gagcaacgta aaaaactgat gaacgtcggt catcgcccgc cactcttgct cgaccacact
     1801 ggcatcggct cgcgtttgaa caactggcgc atcaacggct tttaactcaa gcggcgtatt
     1861 ctcatcggtg ataaaccgtg ccagaagctc actccatgcc gccatatcgg tattgtcggt
     1921 ggcataaact tttagtaatg catcgccctg atgatcaaag aactgaatac tctggcgttc
     1981 gccacgagcc gtgttttctt tgatgtggaa aacactggcc cattgattga gaaacagacg
     2041 taaatccagc gcgcgcggat tgaggatcaa tccggcatgt ccgttcaaat gctggtttgt
     2101 gaacgcacca acttgctcat ggactgcata ttcattacgg caaatacatt tggtttcgcc
     2161 aacactttcg agcgccgcca gaatttcacg gatatcgccg cgcatccgcc acgcatcgtg
     2221 cgtgacgcgt gcaaatgcca gttctgcttc gctgatattc attaaacctg cgatgtcacg
     2281 cgcgtacttt ccgggatttt gttcttttaa ctcaagccag cgtgtgtagt ggttcattgt
     2341 ctctcttcct tccagataaa tgccccgaaa tatcggggca gatgattacc attgataact
     2401 cacgaaaatt tttccgttac gaccatcctg tgggatgcct tgcggcgacc agtactcttt
     2461 atcgaaggcg ttgcccaata ccagagtagt ggtcatgcct ttgagcgcct gctgcccttg
     2521 ataactgacg tagaaatcat tcacaccata gccaggttgt ttgctgtagc tgctgctgat
     2581 atgtgttgag cgatcggcaa acgtaccgac ccaaccaaca gagaagccac tgtgagcgat
     2641 cggaatattc agggtactgg taacggtatc cgggttaatg ctggagatat attccccggt
     2701 atcggtgtct ttgccgcggg tacggttata ggccacatca aggctaaaca gatcagtggt
     2761 atatttcgtc atcacatccc agccccagat tttggcgttc gggacgttat acgacatagt
     2821 tgtcgccgcc gcgaaatcga cggtcgtgga gatataatct ttcgctttgg tatcaaagta
     2881 gctggcttta aattccagag catcattgga caacatcagg tcatcaaaac gcagcccaaa
     2941 accgtactcc tgagtttcgt tagtttccgg acgtaagttc gggtttggca cccaatagtt
     3001 ggtatagaag cgaccaatcg agaagtgttt agaatcgtta tacatttcgc ccatcgtcgg
     3061 ggcgcggaat gcctgagcat atgagccaaa taacatcagc cagttggtcg ggttgatagt
     3121 catccccgca cgagatgacc atttgtcggc atcaacatct ttgtagccgt cgctgctacc
     3181 gcgataactg tcatagcggg ttccgccaag cagggtaatc ggcagatcgc gtaaggtgat
     3241 ctcatcttgt agccagccag agctaaaatc gatttttgct tgcgggaagc ccgtcgtcgc
     3301 gccacccgga tgttgttcct gacgataata ctcaccgcca tatgtcagta agtgagaagc
     3361 gaaactgtcg gcaaatagag tggaacggtt ctccagcctt gctccttttg ttatctgttc
     3421 acgatactcg ccggaactcc ccgtgttttg cgcattaata cggacttccg accagtaaat
     3481 ttttgcatct gcatttaacc agtcgttacc ctgcggggcg agtttataag aaagctgcgc
     3541 atcgcgttga attgttgaac gatcgaccat cgggttgctg ctatcagaag cttcaacggt
     3601 ctgcggattt tttggttcac gcgcgtcgtt gttgtagtaa cgcactaaac cgctcagaga
     3661 ctgggctgaa tcaatttgcc aggtcccttt cgccagcatg ttattaatgg attcgtcatt
     3721 cggcgcggtt tcaccattgc tctggcgtaa atcaccccga tcgcgactgg accaggccac
     3781 aataccatcc agattttcag ttcgcccaaa cgcgctcgcg cctaatccca ggctatggtc
     3841 ccccgtgccg ccagtaccaa agacacgaaa accactgctt tgtccttcct gcaataaatc
     3901 ttttgcatcg accgtatcgt aggagatcac tccacccagc gcgccactgc catacagtaa
     3961 tgctgaaggt ccgcgaacaa tctcaacacg cttgatcagc gccggatcga gaaaagtgcc
     4021 attcaggtgt ccggtatccg ttccctggcg aacaccatcg acaagaacca gcacgccgcg
     4081 atgatcatag ccacgcatat ttacatcctg accgttggtt cgtccggtac catcaagagt
     4141 aattccagga acatgacgca gcaaatcagt ggctgaagta gcagtttgat tttcaggagc
     4201 ggaagtgtcg ataacgctga ccatcatagg cgcttcgaag gaactacgtg cattccccgt
     4261 tgccgtaacg gtcatggttt cagtagcaaa agcaaacgtt ggcaaggtgg cagaaacagc
     4321 caaagccaac aaactcaaac gcaacgaggt aaattgcgga cgtgacatag cgattctcca
     4381 tgaggaaaat aaaaaatgct ggctgcctgg gttaataccg gggtggctgg cgaaaaataa
     4441 atttatttac aattc
//
LOCUS       DADSZD010000253         4441 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.255, whole genome shotgun sequence.
ACCESSION   DADSZD010000253 DADSZD010000000
VERSION     DADSZD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.255"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..2885
                     /locus_tag="J1Z28_004245"
     CDS             <1..2885
                     /locus_tag="J1Z28_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBA7719010.1"
                     /translation="VAVVQAQGRQLVKKGKDFTTLCPFHDEKTPSCVISPEKNLYHCF
                     GCNAGGSVLNWVMQTEKLSLRKAVERLKAELGDNPAVVPLVTKDEPEIFAEDEAGRQQ
                     LLSRVVEFYHHTLLNAPEAVAYLEKRRLNHPELVAAFKLGFANRTLPYRLPAVKSRAG
                     EKIRARLKTAGIMRESGHEHFTGSLVVPVIDLNGQIREMYGRKIERPQRGLPAHLYLP
                     GAHGGVWNEQALIGSSVVVLCESLIDAMSFWVAGFRNVTAAYGVNGFTDEMDQAFRKH
                     GVRQVLIAYDNDPAGNEAAVKLASSLAADGIVTFRVLFPAGMDANGYLCQVAEPERAF
                     SLLLDGAVPVADVVDAESIAPQASAEPAHLTSLAAGVAALPAAAAPAPGVSVKALPEG
                     ELMIALPGERWTIRGLSRKPNAAAMKVNAQVLDTESGVVFADAVDMMSARSRGGYARA
                     AAAELGLAEGDLRRSLGKVLLALERQQAQPEPENAAPEMTPEDREAALELLRDECLAS
                     RIAADMAACGVVGESTNLTAAYLAAVSRKLDRPLAVLIQSSSAAGKSSLMDAVLNLLP
                     PEERLQYSAMTGQSLFYLGETNLQHKILAIAEEEGVRQAAYALKLLQSDGELTIASTG
                     KDDASGNLVTKQYTVKGPVMLMLTTTAIDVDEELLNRCLVLTVNESREQTEAIHAAQR
                     YAQTLEGLLASNEKSYITALHQNAQRLLRPLNVVNPFASQLTFMSDKTRTRRDHMKYL
                     TLIQAIALLHQYQREIKRVEHRGQVVEYIEVTREDIALANRLAHEILGRTLDEMSPQT
                     RKLLTLIQQWVSGSGQAREAMLFTRKQLRDAVQWGDTQLKIHLARLVEMEYLLVHRRG
                     LTFCYELLFDGDGNADAAHLCGLIDVPETPYDVARSGEAVKQSASGRAVVGGQSGKAE
                     TVPDQTPCGLQAGAVGAEEKALFRQNGKTPSRNHTAARPGRSNTEA"
     gene            3079..4179
                     /gene="xerC"
                     /locus_tag="J1Z28_004246"
     CDS             3079..4179
                     /gene="xerC"
                     /locus_tag="J1Z28_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerC"
                     /protein_id="HBA7719011.1"
                     /translation="MTKPKSPGPEALYLGRQNEHTTQRQHMLAWLESLDAAGYSARSV
                     EGYRERLLPFMTWCEARGLLYAPQISLAVLEAYQRHLRSYRRADGNTLAVGGQLNRLS
                     AVKNFFRWLLQRHVILYSPAEMMTLPKEEKRLPAQVFSEQETRQVLGSLDTEKPLGLR
                     NRAILEVLWSTGIRRMEVANLMLPDVDFTRGVVNVRQGKGKKDRVVPVGHVALAWVMR
                     YLRNVRPHLTQRADSGHLFVTYHGKGLSRGTLTQIGGETIREKARIQKPGACHIFRHS
                     MATQMLDNGADTRHIQAILGHEKLETTQIYTRVAIGHLQEVHALTHPEEQDETWTDRH
                     AADAVAEPHPRRERNHAEMERALVSRKRSRRR"
BASE COUNT          933 a         1273 c         1500 g          735 t
ORIGIN      
        1 tggtcgccgt ggttcaggcg caggggcgac aactggttaa aaaagggaag gacttcacca
       61 cgctgtgtcc gttccatgac gagaaaacgc cgtcctgtgt gatctcgccg gagaaaaacc
      121 tgtatcactg cttcggctgt aatgcgggcg gatcggtgct gaactgggtg atgcagacgg
      181 aaaaactcag cctgcgtaag gcggtggaac gtctgaaagc ggagctgggc gacaatcccg
      241 ccgtggtgcc gctggttacc aaagatgagc cggaaatctt cgcggaagat gaagcgggcc
      301 gtcagcaact gctctcccgt gtggtggagt tctaccacca cacactgctg aacgcgccgg
      361 aagcggtggc gtatctggaa aagcgccgcc ttaaccaccc tgaactggtc gccgccttta
      421 aattaggatt cgccaaccgc acgttgcctt atcgtttgcc cgctgttaaa tcacgggcag
      481 gcgagaagat ccgcgccagg cttaagaccg caggtatcat gcgcgagtcc gggcatgaac
      541 acttcaccgg atcgctggtt gtgccggtga tcgatctgaa cgggcagatc cgcgagatgt
      601 acgggcgcaa gatcgagcgt ccacagcgtg gcctgcccgc acacctgtat cttcccggcg
      661 ctcacggcgg cgtgtggaat gaacaggcgc tgatcgggtc atccgtggtt gtcctgtgcg
      721 aatcgctgat cgatgccatg tcgttctggg tcgccgggtt ccgcaatgtc acggcggctt
      781 acggggtgaa cggcttcacc gatgagatgg atcaggcgtt cagaaaacac ggcgtcaggc
      841 aggtactgat cgcctacgac aatgatccgg cgggtaatga ggcggcggtg aaactggctt
      901 cttccttagc cgctgacggg atcgtcacgt tccgcgtact gttcccggcg ggcatggatg
      961 cgaacggcta tctgtgccag gtggcagagc cggaacgggc gttcagtctg ttgctggatg
     1021 gcgcggttcc ggtggctgac gtcgtggatg cggagagtat cgcgccgcag gccagcgcgg
     1081 aacctgcaca cctgacttca ttagctgccg gggttgcggc cttacccgca gccgccgcgc
     1141 ctgcaccggg cgtcagcgtg aaagcgttgc cggaaggtga actgatgatc gccttaccgg
     1201 gagaacgctg gacgatacgg ggcttaagcc gcaaaccgaa cgcggcggcg atgaaggtta
     1261 acgcgcaggt gctggacacg gaaagcggcg tggtgttcgc ggatgcggtg gacatgatga
     1321 gcgcccgcag tcgcggcggt tatgcgcggg ccgcagcggc agagctgggc cttgcagaag
     1381 gcgatctgcg ccgctcgctg ggtaaggtgc tgctggcact cgaacggcag caggcgcagc
     1441 cagagccgga gaatgccgcc ccggagatga cgccggaaga tcgggaggcg gcgctggagc
     1501 tgctgcgcga cgagtgtctt gcgtcacgca tcgcggcaga tatggcggcc tgtggtgtgg
     1561 tgggtgagtc caccaacctg acggcggcgt atctggcggc ggtatcgcgc aaactggaca
     1621 ggccgctggc ggtgctgatc cagtcatcga gcgcggcggg taagtccagc ttaatggatg
     1681 cggtgctcaa tctgctcccg ccggaagagc ggctgcaata cagcgccatg accgggcaga
     1741 gcctgttcta cctcggtgaa accaacctgc aacacaagat cctcgccatc gcggaagaag
     1801 aaggtgtgcg ccaggcggcg tatgcgctga aactgttgca gtcggacggg gaactgacca
     1861 tcgccagtac gggtaaggac gacgccagcg gcaatctggt taccaaacag tacacggtaa
     1921 aaggcccggt gatgctgatg ctgaccacca cggcgatcga cgttgacgag gagctgttaa
     1981 accgctgcct ggtgctgacg gtgaacgagt cgcgggaaca gacggaagcc atccacgcgg
     2041 cgcagcgtta cgcgcagacg ctggaaggtc tgctggcgtc gaatgaaaaa agttacatca
     2101 ccgcgctgca ccagaacgca cagcggctgc tgcgcccgct caacgtggtg aacccgttcg
     2161 cgtcacagct cacgttcatg agcgacaaaa cccgcacccg gcgcgaccat atgaaatacc
     2221 tgacgctgat ccaggcgatc gcgctgttgc accagtacca gcgggagatc aaacgcgtgg
     2281 agcatcgcgg gcaggtggtg gagtacatcg aggtgacgcg ggaggatatc gcgctggcca
     2341 accgcctggc acatgaaata ctcggacgca cactggacga aatgtcgccg cagacgcgga
     2401 aactgttaac cctgatccag caatgggtga gcggcagcgg tcaggcgcgt gaggcgatgt
     2461 tgttcacccg taaacaactg cgtgacgcgg tgcagtgggg agacacgcag cttaagatcc
     2521 acctggcgcg gctggtcgaa atggaatacc tgctggtcca ccgtcgcggg ctgacgttct
     2581 gctatgaact gctgttcgac ggggacggta acgcagacgc cgcgcacctg tgcgggctga
     2641 tcgacgtacc ggaaacgccg tatgacgtcg cccggtcggg ggaagcggtg aagcagtcgg
     2701 cctccggtcg ggctgtggtc ggcggtcagt cgggtaaagc tgaaaccgta ccagaccaga
     2761 caccatgcgg cttacaggcc ggagcggtcg gggcagagga aaaagccctg ttccggcaga
     2821 acgggaaaac cccgtcccgc aatcatactg cggcccgtcc gggccggagc aacaccgaag
     2881 cataagtatg acctcatccg gtcggggtag cggctgcaat agtcggcccc cggtcggcgg
     2941 gcggtcaggt aacgtaaaac caccggaagc cacactctgt aaggcttacg ccgtgaagcg
     3001 gtcggcctgc ctgaaaatca ctgttccatc ctctgttctt ctctctgtcg taccctaatc
     3061 tcaatcagga caatcatcat gacaaagccc aaatcaccgg ggccggaagc cctttatctt
     3121 ggtcgtcaga acgaacacac gacgcagcgc cagcatatgc tcgcctggct ggaaagcctc
     3181 gacgccgcag gctacagcgc gcgcagcgtg gaaggctacc gtgaacggtt gttgccgttc
     3241 atgacatggt gcgaagcgcg cgggctgctg tacgcgccac agataagcct ggcggtgctg
     3301 gaagcgtacc agcgccatct tcgcagctac cgcagggccg acggcaacac gctggcggtc
     3361 ggtggacagc ttaaccgcct cagtgcggtg aagaacttct tccgctggct gctgcaacgc
     3421 cacgtcatcc tctacagtcc ggcagaaatg atgacgctgc cgaaggagga gaaacggctt
     3481 ccggcgcagg tgttcagcga gcaggagacg cggcaggtgc tgggcagtct ggacacggag
     3541 aagccgctgg gactgcgtaa ccgggcaata ctggaagtgc tgtggagcac cgggatcagg
     3601 cgcatggaag tggctaacct gatgctgcca gacgtggact tcacgcgcgg cgtggtgaac
     3661 gtgcggcagg gcaaggggaa aaaggatcgg gtcgtgccgg tgggccatgt ggcgctggcc
     3721 tgggtgatgc gctacctgcg taacgtccgg cctcacctga cgcaacgcgc cgacagcggg
     3781 catctgttcg tgacgtacca cggtaagggg ctgagccggg gaacgctgac gcagatcggc
     3841 ggggaaacca tccgtgaaaa ggcgcggata cagaaacccg gagcctgtca catcttccgc
     3901 cactcaatgg cgacgcagat gctggacaac ggcgcagaca cgcggcacat ccaggcgatc
     3961 ctcggccatg agaagctgga gacgacgcag atctacacgc gggtagccat cggtcactta
     4021 caggaagttc acgcgctgac gcacccggaa gagcaggacg agacgtggac agatcgacac
     4081 gctgcggatg cggtggcaga gccgcatccg cgccgggagc gtaaccatgc ggagatggag
     4141 cgggcgctgg tcagcaggaa gcgcagccgg agacgctgac gcaggccgga agcgtgatca
     4201 tcgccggaca gtctgaaaag ttaggtggcg atccctgtaa ggccgcgggg cgcggtggac
     4261 aggcaaaagg cgcgcctccc tgcgcacctc ttgcccgttc agccccggcg tgcgcggggg
     4321 taaatgccgc ccgccccgcg gaggctgccg cgccccatgc gactatgcag cacggggcaa
     4381 acgtcattca acataatgcg ggggattatg cgcactctcg ccgggtggca atgatggcga
     4441 a
//
LOCUS       DADSZD010000254         4358 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.256, whole genome shotgun sequence.
ACCESSION   DADSZD010000254 DADSZD010000000
VERSION     DADSZD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.256"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..356)
                     /locus_tag="J1Z28_004247"
     CDS             complement(<1..356)
                     /locus_tag="J1Z28_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBA7719012.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTRE"
     gene            complement(471..546)
                     /locus_tag="J1Z28_004248"
     tRNA            complement(471..546)
                     /locus_tag="J1Z28_004248"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(511..513),aa:Thr,seq:ggt)
     gene            complement(553..627)
                     /locus_tag="J1Z28_004249"
     tRNA            complement(553..627)
                     /locus_tag="J1Z28_004249"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(592..594),aa:Gly,seq:tcc)
     gene            complement(744..828)
                     /locus_tag="J1Z28_004250"
     tRNA            complement(744..828)
                     /locus_tag="J1Z28_004250"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(792..794),aa:Tyr,seq:gta)
     gene            complement(837..912)
                     /locus_tag="J1Z28_004251"
     tRNA            complement(837..912)
                     /locus_tag="J1Z28_004251"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(877..879),aa:Thr,seq:tgt)
     gene            1274..2224
                     /gene="coaA"
                     /locus_tag="J1Z28_004252"
                     /gene_synonym="panK"
     CDS             1274..2224
                     /gene="coaA"
                     /locus_tag="J1Z28_004252"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBA7719013.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2253..3218)
                     /gene="birA"
                     /locus_tag="J1Z28_004253"
     CDS             complement(2253..3218)
                     /gene="birA"
                     /locus_tag="J1Z28_004253"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBA7719014.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3215..4243)
                     /gene="murB"
                     /locus_tag="J1Z28_004254"
     CDS             complement(3215..4243)
                     /gene="murB"
                     /locus_tag="J1Z28_004254"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBA7719015.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNTGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1120 a         1085 c          988 g         1165 t
ORIGIN      
        1 tgctcacgag tctgcggcat cgggccgtca gtcgcagcaa ctaccaggat cgcgccatcc
       61 atctgagcag caccggtgat catattttta acatagtcgg cgtgccccgg gcagtctacg
      121 tgtgcgtagt gacgggtcgg ggtgtcgtat tcaacgtgag aagtgttgat ggtgatacca
      181 cgagcttttt cttccggcgc gttatcgatc tggtcgaatg cgcgagcagc accgccgtag
      241 gttttagcca gtacggtagt gattgcagcg gtcagcgttg ttttaccatg gtcaacgtgg
      301 ccgatagtac cgacgttaac gtgcggtttt gtacgttcaa acttttcttt agacatcgat
      361 tgtccctcta agacacggat aaatcggtga tatcaccaca tcaaccaggc aacatgcccg
      421 acttgttgaa tgcaataaac agaaggaaaa aacagggagg agaaaagaag tggtgctgat
      481 aggcagattc gaactgccga cctcaccctt accaagggtg cgctctacca actgagctat
      541 atcagcacat cttggagcgg gcagcgggaa tcgaacccgc atcatcagct tggaaggctg
      601 aggtaatagc cattatacga tgcccgcatc ctggaactcg gctacctgat tttcattctg
      661 cactgaatat cgagagaagc tctctttatt cgagccggta agcgaactta tcgtctcggg
      721 ctacgccatc gcgtggccga aattggtggt gggggaagga ttcgaacctt cgaagtctgt
      781 gacggcagat ttacagtctg ctccctttgg ccgctcggga accccaccgg acttgatggt
      841 gccgactacc ggaatcgaac tggtgaccta ctgattacaa gtcagttgct ctacctactg
      901 agctaagtcg gcatcaagta gcgcgcattc tatggagaca tgcgagttca tgcaactaaa
      961 aaattgcata atttgttttg ttggtcacat tttgttcgac acgatgaaga aacagccggg
     1021 ttaagagcag attagcatac tttttcagca tcactctgct taacaatgtg cgccagctaa
     1081 atcgcaattc tttttatcag atggaatatc tgtcacattg cttttcaacg atagcttcct
     1141 gggagagatt ttttcttatt attcctcccc atctggtgtt accctcctgc ccattaaccc
     1201 attcaacaga actgtgacgc gccatggcaa atatcgcttt gccgatagag ctatgaccgc
     1261 cagaaacatg cttatgagta taaaagagca aacgttaatg acgccttacc tacagtttga
     1321 ccgcaaccag tgggcagctc tgcgtgattc cgtacctatg acgttatcgg aagatgagat
     1381 cgcccgtctc aaaggtatta atgaagatct ctcgttagaa gaagttgccg agatctattt
     1441 acctctgtca cgtttgctga acttctatat aagctcgaat ctgcgccgtc aggcagttct
     1501 ggaacagttt cttggtacca acgggcaacg cattccttac attatcagta ttgctggtag
     1561 tgtcgcggtg gggaaaagta caaccgcccg tgtattgcag gcgctattaa gccgttggcc
     1621 ggaacatcgt cgtgttgaac tgatcactac agatggcttc cttcacccta atcaggttct
     1681 gaaagaacgt ggtctgatga agaagaaagg cttcccggaa tcgtatgata tgcatcgcct
     1741 ggtgaagttt gtttccgatc tcaaatccgg cgtgccaaac gttacagccc ctgtttactc
     1801 gcatcttatt tatgatgtga tcccggatgg agataaaact gttgttcagc ctgatatttt
     1861 aattcttgaa gggttaaatg tcttacagag cgggatggat tatccacacg atccacatca
     1921 tgtatttgtt tctgattttg tcgatttttc gatatatgtt gatgcaccgg aagacttact
     1981 tcagacgtgg tatatcaacc gttttctgaa attccgcgaa ggggctttta cagacccgga
     2041 ttcctatttt cataactacg cgaaattaac taaagaagaa gctattaaga cagccatgac
     2101 attgtggaaa gagatcaact ggctgaactt aaagcaaaat attctaccta ctcgcgagcg
     2161 tgccagttta atcctgacga aaagtgctaa tcatgcagta gaagaggtca gactacgcaa
     2221 ataattttca ggggagctaa tgctcccctt tcttattttt ctgcactacg cagggatatt
     2281 tcaccgccca tccagggttt tattattcca tcctgctcaa gtaataaagc accctgtttg
     2341 tctattccgc gtgaaatgcc aaatatttct ttatcaccaa tgataagttt cactgggcga
     2401 ttaataaaat tatccaactt ttcccagcgc gaaagataag gtgccaatcc ttcttgctcg
     2461 aagagttcca gcgccgcgcg taattcacgt atcagcatgg ccgcaagagt attacgatcg
     2521 agattgatcc cggcttcctg cagcgtgatc cacccttgat taacgacact ctcttcaaca
     2581 cgacgcattg ccatgttgat ccctgcacca atgactattt gcgccgcatc gccggttttg
     2641 ccagtcagct ccacaagaat gcctgccagc ttgcgatcct gcagatagag gtcattaggc
     2701 catttaacac gaactttatc tgcacccagc ttgcgtaata cttccgccat cacgataccg
     2761 ataaccagac ttaaaccgat cgccgccgcc gggccctgtt ccagacgcca gaacatcgac
     2821 aaatataagt tcgcgccaaa aggcgaaaac catttccgac cacgacgacc acgaccagca
     2881 tgctggtatt ctgcaacaca ggcatcgcca gatttaagct ctccgatgcg gtccaggaga
     2941 tactgattcg tggagtcaat aactggcagc acggctacac tgccgtcatc cagctgactc
     3001 aatatctttt cggcattaag taactggatt ggctcgggca agctgtatcc tttacccgga
     3061 acagtaaata catcaacgcc ccagtcacgc agtgtctgaa tgtgtttatt aatagccgcc
     3121 cggctcattc ccagcgtttc acccaactgc tcgccagagt gaaattcacc gttcgctaac
     3181 agggcgatca atttcagtgg cacggtgtta tccttcatga aattgtctcc actgcgctca
     3241 cttcacctga tgcaccaata aagcggactt caggctcaag ccagacatta aatttttcac
     3301 ccactttttg tcttacatga tgcgccagct gtacaacatc ttcgcttttt gcatcgtgct
     3361 catttatgag aaccagcgcc tgttgacggt gtaccgcagc cccaccaatt tgcgtccctt
     3421 taagctggca ctgatcaatc agccaacccg cggccagttt tactgaacca tccacctggg
     3481 ggtaatgtgg ggctgttgga aattgcgcca acaacgcgtt agcagtttcg gcagaaacaa
     3541 caggattttt gaagaaacta cccgtattac cgttcacttt tggatcaggg agtttggtgg
     3601 tgcgcatatg acacaccgca tcaaatactt gctgtggcgt tactgttgta ggatccagac
     3661 gagttaagtc accatacgtt agtacaggtt gccactcttt tggcagacgc agacctacgg
     3721 ctacaatggc gaagcgatcc tggtattcat gtttaaaaat actgtcgcga tagccaaaac
     3781 ggcactcttt ggcagttaag cgtacttgct tgcctgtcgc cagttcaaca caatcaacat
     3841 aagcgcaaac tcgctgtaat tctacgccat aagcaccaat attctggata ggtgatgagc
     3901 cgacacaacc aggaattaat gccagatttt ccagaccagg cataccttcc tgcaaagtgt
     3961 attttaccag gcgatgccag ttttctccgg ctcctacatg taaataccac gcatcaggtt
     4021 catcatgaat ttcgatacct ttgatccggt tgatgatcac cgtgccgcga tagtcctcca
     4081 gaaaaagtac attacttcct tcacccagaa taagaacggg ttgtccttct gcggttgcat
     4141 gctgccaggc attgagtaat tgttgttcgt cttcggcaca tacaatgtgc tgagcattat
     4201 gatcaatgcc aaatgtgttc cagggtttta aggagtggtt catagctgct ttcctgatgc
     4261 aaaaacgagg ctagtttacc gtatctgtgg ggggatggct tgtagatatg atgacaggaa
     4321 gagtttatag acacgcaaaa aggcccagtc ttccgact
//
LOCUS       DADSZD010000255         4191 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.257, whole genome shotgun sequence.
ACCESSION   DADSZD010000255 DADSZD010000000
VERSION     DADSZD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.257"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..543
                     /locus_tag="J1Z28_004255"
     CDS             <1..543
                     /locus_tag="J1Z28_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBA7719016.1"
                     /translation="SNGMFNFIVMQTLFYLPFFILGALAFIFPHLKALFTTPSRGCTL
                     AAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVNVVFSFGHRLLNFQSARVTY
                     FVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLIFVVGIAIILYEIHLRIPLL
                     KFLFSGKPVVKRENDKAPAR"
     gene            complement(551..2032)
                     /locus_tag="J1Z28_004256"
     CDS             complement(551..2032)
                     /locus_tag="J1Z28_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HBA7719017.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDVMTHRYLRKMESSPFIHHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(1974..2507)
                     /gene="ymdB"
                     /locus_tag="J1Z28_004257"
     CDS             complement(1974..2507)
                     /gene="ymdB"
                     /locus_tag="J1Z28_004257"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBA7719018.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2602..2913)
                     /locus_tag="J1Z28_004258"
     CDS             complement(2602..2913)
                     /locus_tag="J1Z28_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7719019.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSSAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3034..3366)
                     /gene="csgC"
                     /locus_tag="J1Z28_004259"
     CDS             complement(3034..3366)
                     /gene="csgC"
                     /locus_tag="J1Z28_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBA7719020.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3425..3883)
                     /gene="csgA"
                     /locus_tag="J1Z28_004260"
     CDS             complement(3425..3883)
                     /gene="csgA"
                     /locus_tag="J1Z28_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBA7719021.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3924..>4191)
                     /gene="csgB"
                     /locus_tag="J1Z28_004261"
     CDS             complement(3924..>4191)
                     /gene="csgB"
                     /locus_tag="J1Z28_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBA7719022.1"
                     /translation="GSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDASISQG
                     AYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1056 a         1059 c          942 g         1134 t
ORIGIN      
        1 agtaacggca tgttcaattt tattgtcatg caaacgttat tttatttgcc gttctttatc
       61 ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
      121 ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca gcgctatggc
      181 agtggcgatg cctggatgta tgaaaccgag tcggtgatca ctatggtcct cggtctgtgg
      241 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
      301 acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt aacgctgttt
      361 ttcggcgcat acattacacc acacatcacc tccaactggc ttggttttct ctgtggcctg
      421 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccattattg
      481 aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
      541 taagtcgcgt ttacaataac cattccacgg gcaatatcga cgccagtttg accataaccc
      601 gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat
      661 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc
      721 tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
      781 tttcagtatt gagcaacgtt gaacgcggat cgaaattgaa agagccgata aacaccgttt
      841 taccatcgat actaaaggtt ttagcatgca ggctggcccc tgaattaccg gttatgccgc
      901 gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ctcatataat tccaccccat
      961 agcggagcaa ttttttgcgc cagcgcgcgt atcctgcatg gacgacagca acatcgttag
     1021 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     1081 gcgccacacc tgcacgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     1141 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     1201 cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc
     1261 catcaaccag atgatgtata aacggactgg attccatttt gcgtaagtaa cgatgcgtca
     1321 tgacatcgtt atgccaggag gcgggtaact cgatgcgatc tgccatttca ccttccggga
     1381 catccagcac ctgctgtaag ggggaaaccg atttgcaata ccaatagcgg gcgaaatcat
     1441 cggcaacgtc ctctaccacg ggtcctattg ccatgacatc taaatccgaa aaaagtggct
     1501 cttcccctgc accaaaatag gcatcaccaa tatttcgtcc tcccaccagc gtcaccacgc
     1561 catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat
     1621 aaccaagcgg acgtagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcgtg
     1681 gatggctgtc aagcaagcgt aaaatgttat caagtccggg cgtattgttg tcgtccagca
     1741 acaaacgtac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac agtaaccgac
     1801 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcag
     1861 ccagacgata ccgggcggca aacgcatcca gacttttctc cagaggaaaa agtccacact
     1921 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccgaggc aaatcattca
     1981 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     2041 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg
     2101 atttcagccg ctgccgcacg aggataacca taaaccccag tactgattgc aggaaaagcc
     2161 actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc atcctgcaaa
     2221 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg cactacggct
     2281 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     2341 tgctgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     2401 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     2461 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttac
     2521 accttcttgt ttcctaagtg tacagcaggc tttttggtta aatcgcattt ttctgctcag
     2581 caacgtgagt tgttcagaaa cttagttata ttcgatgttc attacagcca gctttttctg
     2641 tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttaacat tgccgctcat
     2701 taacgctttg ctggaatagg ttttacgcgg aacagaacca ttctgtgggc agttcatttc
     2761 tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc cataaaaatg
     2821 gatcacgccc ccttgcgcgc tgcttcctgc aatggcaata aaaggaaaaa acaaactgcc
     2881 gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa tgattaatca
     2941 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     3001 ccaggttcat cttatgctcg atatttcaac aaattaagac ttttctgaag agggcggcca
     3061 ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt tcacccgatc
     3121 gtccggggaa atatttaaac tcaacttcgt caaagcaatg agttgattag caggcaatga
     3181 aagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca gggacaatat
     3241 ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg tgtacatatc
     3301 cccttgctgg gtcgtattaa aggttatctg actggaaagt gccgcaagga gtaataacgc
     3361 attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctttc atactgatga
     3421 tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtg acgttaacgc
     3481 tggagttaga tgcagtctgg tcaacagcag caccgttacc gccaccgaac tgtttaacag
     3541 tcatagtaga atctttacca ttccactgat caagagtagc gctgttgccg aaaccacgct
     3601 gagtcagatc gattgagctg tcatcagaac cttggccaac atctgcgccg ttaccgccgc
     3661 cgtgctgggt aatggtcaga tcagagttac gggcgtcagc ttgcagagca agagcagagt
     3721 taccgccacc gtactggtaa atattcagct ctgaattcgg gccgctgtta ttaccgccac
     3781 caccgtggtt gccaccgccg ccgccgtact gaggaacaac acctgccaga gcgctaccgg
     3841 agaatacgat tgctgcaatt gctgctactt ttaaaagttt catgtaaaac ccccatcgga
     3901 ttgatttaaa agtcgaatgg aaattaacgt tgtgtcacgc gaatagccat ttgcgactgt
     3961 ctctgcacta caattgccgt tttttgagta ccatactgtg taatatttgc tttattacca
     4021 gaacctttct ggataatcat cgcagtatta ccataagcac cttgcgaaat actggcatcg
     4081 ttggcactgc ccgcctgatc aatatatgca aggttataat ctcctgtctg atcaatcttt
     4141 gcccggttgc tactaccttc ttgcgcaaca accgccaaaa gttttgagcc t
//
LOCUS       DADSZD010000256         4190 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.258, whole genome shotgun sequence.
ACCESSION   DADSZD010000256 DADSZD010000000
VERSION     DADSZD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.258"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(24..2000)
                     /gene="speA"
                     /locus_tag="J1Z28_004262"
     CDS             complement(24..2000)
                     /gene="speA"
                     /locus_tag="J1Z28_004262"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBA7719023.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2009..2140)
                     /gene="yqgB"
                     /locus_tag="J1Z28_004263"
     CDS             complement(2009..2140)
                     /gene="yqgB"
                     /locus_tag="J1Z28_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBA7719024.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2276..2491
                     /locus_tag="J1Z28_004264"
     CDS             2276..2491
                     /locus_tag="J1Z28_004264"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719025.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLFSTRLEVATRKKRRVLFI"
     gene            2795..3949
                     /gene="metK"
                     /locus_tag="J1Z28_004265"
     CDS             2795..3949
                     /gene="metK"
                     /locus_tag="J1Z28_004265"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBA7719026.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1050 a         1145 c         1018 g          977 t
ORIGIN      
        1 ccccgccagc ggaataatct taattattca tcttcaagat aggtataacc gtacaaacct
       61 gcctcgaact cttcaaggaa ctgttgttgc agttcagcat caagatcggt tttcttcacc
      121 tgatcgcgga actgggttaa cagcgttttc ggatcgagct gcacatattg cagcatgtcc
      181 gccacggtat cgccttcgtc agacagttct acctctacgc taccatcagg gaagacgaac
      241 acgtcaaccg cttcggtatc accgaacagg ttgtgcatgt tgccgaggat ctcctgatat
      301 gcgccgacca taaagaaacc gagcatcggc ggattctccg gatcgtactc cggcataggc
      361 attgtcgtgg caataccgtc accatcaata tagtggtcga tagcaccgtc tgagtcacag
      421 gtaatatcca gcagcacagc gcggcgttcc ggcacttgat ccagcccttc cagcggcaga
      481 accgggaaca actggtcgat cccccacgca tccggcatcg actggaacag cgagaagttg
      541 acgtacattt tgtccgccat acgttcctgc agctcgtcga taatcggacg atgagcacgg
      601 ttttgcggat ccagctgctt ttgcacttca tggcacatgc tcaaataaag ctgctcagcc
      661 catgcgcgtt cttgcaggct aaaggtgccg gaagagtagc cgatatgaat gtcgtgcaga
      721 tccatctggc tgtcgtgtaa ccattcacgc agagaacggc gagttcccgg ttcgtgcatt
      781 tcctgccagg tttcccacat gctttgcagg gcgcgcggtg catcttctgc aggcgcggtc
      841 ggcaccgtgt attcgttacg ttccacgccg atgatattcg acaccagcac ggtgtgatgc
      901 gcagtcaccg cacgacccga ttcggtgatt accgtcggat gtggcagacc attttcttca
      961 cacgcatcac caatcgccca gatgatgttg ttggcgtatt cattgaggcc gtagtttacg
     1021 gagcagtcag actgagaacg ggtgccttca tagtccacgc ccagaccgcc gccgacgtcg
     1081 aagcactgaa tattgacgcc cagcttgtgc agttccacat agaaacgcgc ggattcacga
     1141 acgcctgtcg cgatatcgcg aatattcgcc atctgcgaac cgaggtggaa gtgcagcagt
     1201 tgcaggctgt cgagacgccc ggcttcacgc agcgtttcaa ccagttgcag tacttgagtc
     1261 gccgccaggc caaacttcga tttttccccg ccggaggact gccatttacc cgagccctgc
     1321 gaagccagac gtgcacgcac gcccagacga ggaacgacat tcagacgttc tgcttcatcc
     1381 agcacaatgg caatttctga catcttctca atgaccagat agaccttgtg gcccatcttc
     1441 tcgccaatta atgccaggcg gatatattcg cggtccttat aaccgttgca gacgatgacg
     1501 ctacgggtca tgccagcatg tgccagcacc gccatcaact cagctttgga accggcttcc
     1561 aggcccagcg gttcgcccga atgaattagg gactcaatca cgcggcggtg ctggttaact
     1621 ttgatcggat aaacgaggaa gtaatcgccg ttatagccgt aggattcccg cgctcgtttg
     1681 aacgcggcgt taatggaacg caaacggtgc tgcaggatct gtgggaaaca gaacagtgca
     1741 ggcagacgct gaccctgtgc ttcgcgagtt ttcactaact gcgcgagatc gacgcgagct
     1801 tccgggacgt ccgggtccgg gcacacgctg atgtggccca gctcgttaac gtcatagtag
     1861 ttattgcccc accaggcaat attgtaagta cgcagcatct tgctggcttc ctgggagctc
     1921 attgcaacct cctgcatgga gcgtagtaca ccgtgttcgc ccgctgacga aggcaaaccc
     1981 atagacatgt cgtcagacat agcgaacctc aaattatttt attaagtgta aaacagttaa
     2041 cgactatcgc agcctggaac tgcgataaca acccataagc acatggattt tccagcagtg
     2101 aatgctgacg ctccaactgc gcaaccggtt tctttttcat aacattatta agcacataac
     2161 cgaacgtaag tgtgacagtt cggcgaaacc acgagaaaac tcttgttttt acaagagcgc
     2221 ccttgttcag tcctcagtag ctgtaaccag ctcttgaatc ctgagaagcg ccgagatggg
     2281 tataacatcg gcaggtatgc aaagcagaga tgcagagtgc ggggaacgaa tcttcaccag
     2341 aacggtgaga caggttaagc agcagacaac ggttcattat ttcgtatcac ctccacggcc
     2401 gcctgttaag acgaacccac aagccaaaac tctgttttca acccggctgg aagtggcaac
     2461 acgaaagaaa cgtcgtgtgc tttttattta agccgcgcgc cgcgttttat accccacaat
     2521 ggcagaaaat tgcaaaagat aaatacgcag aatgccagca ttgtcaggaa aaatttccag
     2581 ccacgttttt aacagaatga gacacgattc aaaaaaaagt ggaaattggg tgaagaactg
     2641 acctaaaata gccatccaga tgttaatcca tccataccga ttaacactca gactgccagt
     2701 gtttttaacc tgcagagtcg tggtaggatc cgctaccaca gaaaatccac acaacagttt
     2761 gagctaacca aattctcttt aggtgatatt aaatatggca aaacaccttt ttacgtccga
     2821 gtccgtctct gaagggcatc ctgacaaaat tgctgaccaa atctctgatg ccgttttaga
     2881 cgcgatcctc gaacaggatc cgaaagcacg cgttgcttgc gaaacctacg taaaaaccgg
     2941 catggtttta gttggcggcg aaatcaccac cagcgcctgg gtagacatcg aagagatcac
     3001 ccgtaacacc gttcgcgaaa ttggctatgt acattccgac atgggctttg acgctaactc
     3061 ctgtgcggtt ctgagcgcca tcggtaaaca gtctcctgac atcaaccagg gcgttgaccg
     3121 tgccgatccg ctggaacagg gcgcgggcga ccagggtctg atgtttggct acgcaactaa
     3181 tgaaaccgac gtgctgatgc cagcacctat cacttatgca caccgtctgg tacagcgtca
     3241 ggctgaagtg cgtaaaaacg gcactctgcc gtggctgcgc ccggacgcga aaagccaggt
     3301 gactttccag tatgacgacg gcaaaatcgt tggcattgat gctgtcgtgc tttccactca
     3361 gcactctgaa gagatcgacc agaaatcgct gcaagaagcg gtaatggaag agatcatcaa
     3421 gccgattctg cccgctgaat ggctgacttc tgccaccaaa ttcttcatca acccgaccgg
     3481 tcgtttcgtt atcggtggcc cgatgggtga ctgcggtctg actggtcgta aaatcatcgt
     3541 tgatacctac ggcggcatgg cgcgtcatgg tggcggtgcg ttctccggta aagatccatc
     3601 aaaagtggac cgttccgcag cctacgcagc acgttatgtc gcgaaaaaca ttgttgctgc
     3661 tggcctggcc gatcgttgtg aaattcaggt ttcctacgca atcggcgtgg ctgaaccgac
     3721 ttccatcatg gtagaaactt tcggtactga gaaagtgcct tctgaacaac tgactctgct
     3781 ggtacgtgaa ttcttcgacc tgcgcccata cggtctgatt cagatgctgg atctgctgca
     3841 cccgatctac aaagaaaccg cagcatacgg tcactttggt cgtgaacatt tcccgtggga
     3901 aaaaaccgac aaagcgcagc tgctgcgtga tgctgccggt ctgaagtaat ctttcttcac
     3961 ctgctttcaa aggccagcct cgcgctggcc tttttctttt acctcaccgc agtggacagg
     4021 cgttcacgcc gcatccggca aaaaaccgcc cgcacaataa catcattctt cctcaacacg
     4081 ttttaccgca aattatcatc actactgaaa tcgattacac caaccacacc agccaaatat
     4141 ttgtaatatt ttcatattat tattcagctt tcacagttgt tacatttctt
//
LOCUS       DADSZD010000257         4180 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.259, whole genome shotgun sequence.
ACCESSION   DADSZD010000257 DADSZD010000000
VERSION     DADSZD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.259"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(475..1332)
                     /gene="sitD"
                     /locus_tag="J1Z28_004266"
     CDS             complement(475..1332)
                     /gene="sitD"
                     /locus_tag="J1Z28_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBA7719027.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(1329..2186)
                     /gene="sitC"
                     /locus_tag="J1Z28_004267"
     CDS             complement(1329..2186)
                     /gene="sitC"
                     /locus_tag="J1Z28_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBA7719028.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2183..3010)
                     /gene="sitB"
                     /locus_tag="J1Z28_004268"
     CDS             complement(2183..3010)
                     /gene="sitB"
                     /locus_tag="J1Z28_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBA7719029.1"
                     /translation="MMQSAGIVVNDVAVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTTENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(3010..3900)
                     /gene="sitA"
                     /locus_tag="J1Z28_004269"
     CDS             complement(3010..3900)
                     /gene="sitA"
                     /locus_tag="J1Z28_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBA7719030.1"
                     /translation="MLLGGLALTCSIAFQASATEKFKVITTFTIIADMAKNVAGDAAE
                     VSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNGVPEVIVSS
                     GVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQRNADTYKA
                     KITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINADQQGTPQQV
                     RKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTENGPVPTYID
                     LLKVTTSTLVQGIKAGKREK"
BASE COUNT         1154 a         1034 c          977 g         1015 t
ORIGIN      
        1 aaatagtata aatgcaatga taatgtttaa taagacaatc agcttgcttt gtattttcat
       61 ttcggaatac tgttaagtaa ctttaataat cctaacaata gatcggattt ttcttagagt
      121 caatggtccc cgtttttgct tgcaaagcaa atgttttggt agttataagt tgtcttgata
      181 tagtcatgca tgcaacttgt tccgtgaaga gattaaagat tggaatataa cgcgaaaatt
      241 gctcatagtt caccaataca gctataaatc gtaaagagaa ccgtggctcg ttgtatgcaa
      301 aaacgtgcca cggttggctg atgcactttc gagagtgcat gtattgaatt atttccagct
      361 attatcgatt ttacgtattt tttgcatgat agaattcgta cctcctccca acgaccttcc
      421 atgactttcc ggactgctat aggctggcgg acgatgatgt cctcggatgt tgtgctaatt
      481 gggtgataac agatcctgtg tatgggagtt tccctttttg cgtgcgttga tgctggtaac
      541 ggcaaacgtc atgatgaaca aaaccgcgaa taagacgaca atcgtcggag ctggtgcgct
      601 gtcgataaaa aaggacaacc agacaccagt cattgacact attaccgata tgccagtcgc
      661 cagtaggagc gcaatatgaa agcgttgtgt caggagtacc gcaatggcgc caggcgcaat
      721 gagcagagaa atggagagaa tgatacctac cgctttcagc gtcgccacaa tggtcagaga
      781 gaccatgcac agcagcccat aatgcagcca tctcgtatgc aaaccgctta cctgcgcctg
      841 ttgataatca aagctgaaca gcaaaaaatc tcgccatttt acgctaataa caagtgtaac
      901 cagcccagca ataatcaccg tctggattat atcgccgatg gttatcccga gcatgtcgcc
      961 gaaaagaata tggtcaagat gtacgtctgg tttgactgca atatacaaaa tcagacctgc
     1021 ggcaaacatg ccagaaaaaa caatccccat cactgtatct tgcttgattc ggctgttgtc
     1081 tttcaggtat cccgtcgcca ccgcgcagaa tactccggcg acgaatgccc ctgtagccaa
     1141 aggtaacccc agaatccagg caagcacgat gccaggaaaa accgcatgac tcatcgcgtc
     1201 acccatcagt gcccagcctt tcaggaccag aaagaccgac agcagcgcac agggtatgga
     1261 aactaccagt gaaatcagca gtgcgtgact cataaaggtg aactgcagtg gctccagtaa
     1321 taatgccatc atgagcgatc ctccagcgcc ttatgcgcac gccgacgatt tgccaaaagc
     1381 ccatgggtgg gcgcaaagac aaatgctaaa agaaacagca gtgtctgcgc caccacgatg
     1441 attccacctg tcgcgccgtc cagaaagtaa ctgacccacg cgccgaggaa actggttaca
     1501 ctgccaatag ttacagcaat catgagcagg cggggaaaac gatcggttaa caaccatgct
     1561 gttgctccgg gagtcaccac cagacagatc accagaaagg caccaaccgt ttgcagcgcc
     1621 gcaacggtcg aaaccgacag cagggtgaag aagaggagtt ttaatctgcc cggatgcaat
     1681 ccaatagcac gggcgtgatt ctcgtcgaaa aaggtcacca taagatcttt ccatttgaaa
     1741 aaaagcacga tgatcgagag aatgccaatg atagtgagtt gcaggatatc cgccggatcg
     1801 attgccagaa tattgcccag gacgatggtc tgaatgttca cagatgtcgg gttcaatgac
     1861 accataaaca gccccaggcc aaaaaaggac gagaagatca ggccgatgat ggcatcttct
     1921 tttaagcggg tgcgttggtt aagaaacagc atgctgcccg cagccagtcc gccggaaaag
     1981 aaggctccca gagaaaacgg aagccccagc atatatgccc ccgcaacacc cgggacaata
     2041 gagtgcgaca acgcatcgcc aatcagcgac cagcctttta gcatcagata gcatgacagg
     2101 aaagcgcaga gaccgccgac catcgccgag acccacatcg cattgagcat atattcatag
     2161 ctgaagggtt ccagtagcac gttcatcttt cttccctctg caccgctgac ggtcgatgag
     2221 ccacaaaagg gcgttcatcg tcagtaataa tgctttcttc cgagccgtta agtgtgacat
     2281 ggcgcagtac gccgctaaaa gccagctcca ggttctcggt tgtaaaagtg gtgtctgtcg
     2341 gcccgcttgc caggacggtg cctttgacca tgaccgtata atcgcaaaat gtcgtgacgg
     2401 atccgagatt gtgcgttgag acaagcatcg ttttgccttc cgcgcgtaac tccctcaaca
     2461 ggctgatgat tttggcttcg gttttcacat caacgccagt aaaaggttca tccagcagga
     2521 tcacatcacc ctgctgtgca atcgctctcg ccagaaagac gcgtttcttt tgtccaccgg
     2581 aaagctcgcc gatttgtcga tggcgaaaat tcaccatatc gacacgttct agcgcatcgg
     2641 tgacaatttg acgatccctc tttttggcga tacgcaacat acccatatgc ccatagcggc
     2701 ccatcatcac cacatcttcc accagtacag gaaatgacca gtcaacctct tctgactgag
     2761 ggacgtaggc aaccaggttt ttctgtaacg cctgtcgcgt gggaataccc agaacagata
     2821 tctttccgct ggtcagacgc acaaatccca taatcgcttt aaacaacgtc gattttccgg
     2881 aaccgtttac cccaaccaga gcggcaatag atccacttgg tactgtgaag gatgcatcgc
     2941 gcagtgctgt gtgcccgtta cgccaggtga cagcaacatc attaacgaca atgcctgcag
     3001 attgcatcat tatttctccc ttttcccggc tttaattccc tgtaccagcg tactggtagt
     3061 aaccttaaga aggtcgatat acgtcggtac cgggccgttt tctgtgctca gggaatcgac
     3121 atagagcaca ccgccgtagt gtgcgccggt ttcacgcgca acctgacgcg ctggtttatc
     3181 ggaaatcgta ctctcgctaa agactgccgg gatatgattt ttcttaacta tatcaacaac
     3241 cttacgtacc tgctgcggtg ttccttgttg atcggcatta atcggccaca gataaagctc
     3301 ttttagcccc aaatcgcgtg cgagataaga aaaagcccct tcactggtga ccatccatcg
     3361 ctgattctca gggagttccg taatctgctt acgcaggggg gcaagggttt gggtaatctt
     3421 ggctttataa gtatcggcat tgcgttggta ggtttgtgca tttgccggat cgtattttat
     3481 caacgcatca cgaatattat cgacgtaaat cagagcatta tctggcgaca tccatgcatg
     3541 ggggttaggt ttgccctcat agggtccttc ggtgatccct acaggcgtca cacccgaaga
     3601 gacaattact tctggaaccc cattgagatg ctggtaaaag cgttggaacc acaattccag
     3661 attcatacca ttggcgagaa tcagttgtgc cccctgcgca cgtttaatat cgccaggggt
     3721 aggctgatac tcatgaattt ctgcaccagg cttggttatg gatgagactt ctgcagcatc
     3781 tcccgccacg tttttggcca tatccgcgat gatggtaaat gttgtaatga ccttaaattt
     3841 ttcagttgca cttgcctgaa atgcgatcga gcaggtgagt gcgaggcccc ccaagagcat
     3901 ggttactttt tttattgagc gcataattta gtacctatag tgagcaaaat tagaacaaac
     3961 atagcaacgg ctatgtttta tagcataacc tatacagaat gaaaatgatt atcattaaca
     4021 tctttgagtc ccaaaacagg gattagtttg tttcctgctg gatgaaagga ctcttgggat
     4081 agcacatgcc gcacgaagag cagcattaca tgatgttttg tcgaatgagc tacgaatcct
     4141 gatagaaata cgaaacaagc tagcacatgg acaatggatc
//
LOCUS       DADSZD010000258         4171 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.260, whole genome shotgun sequence.
ACCESSION   DADSZD010000258 DADSZD010000000
VERSION     DADSZD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.260"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            253..1002
                     /locus_tag="J1Z28_004270"
     CDS             253..1002
                     /locus_tag="J1Z28_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719031.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSIYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            1135..2397
                     /locus_tag="J1Z28_004271"
     CDS             1135..2397
                     /locus_tag="J1Z28_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7719032.1"
                     /translation="MKANFTLSDGDKAVTDADGKAKVTLKGTKAGAHTVTASMVGGKS
                     EQLVVNFTADTLTAQVNLNVTEDNFIANNIGMTRLQATVTDGNGNPVEGIKVNFRGTS
                     VTLSSTSVETDDQVFAEILVTSTEVGLKTVSASLADKPTEVISRLLNAKVDVNSATIT
                     SQEIPEGQVMVAQDIAVKAHVNDQFGNPVTHQPATFSAAPSSQMIISQNTVSTNTQGV
                     AEVTMTSERNGSYTVKASLANGASLEKQLEAIDEKLTLTSSPLIGVNAPKGATLTATL
                     TSANGTPVEGQVINFSVTLEGATLSGGKVRTNSSDQAPVVLTSNKVGTYTVTASFHNG
                     VTIQTQTTVKVTGNPSTAHVASFIADPSTIAATNSDLSTLKATVEDGSGNLIEGLTVY
                     FALKSGSTTLTSLTAVTDQNGIATTNVR"
     gene            2617..3093
                     /locus_tag="J1Z28_004272"
     CDS             2617..3093
                     /locus_tag="J1Z28_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin"
                     /protein_id="HBA7719033.1"
                     /translation="MQSGTNVPYMKISAIDYSQNINGDYKATVTGGGEGIATLIPVLN
                     GVHQAGLSTTIEFISAETRPMTGTVSVNSANLPTASFPSQGFTGAYYQLNNDNFAPGK
                     TAADYSFSSSASWVGVDATGKVTFKNDGDSNTVIITAPPRSGGAIYQTVPPESRSV"
     gene            complement(3221..>4171)
                     /locus_tag="J1Z28_004273"
     CDS             complement(3221..>4171)
                     /locus_tag="J1Z28_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBA7719034.1"
                     /translation="PDQIGDAMAIFPVIRALELHKIKHLLIVTSTINLEVFNALKLEQ
                     TKLTLVTMTMQDHATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKT
                     NFQVVGLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRSIYELPLSDDV
                     LDEVREEMRSLGSYIALNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGED
                     KARALVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYAD
                     YKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT         1235 a          959 c          911 g         1066 t
ORIGIN      
        1 atacataaca caaatatcac tcaggtacac tactcaaacc acgctgggat ttttcctcaa
       61 gttataatca tcaccccgaa aatcattcgg catttactca ttaaatagtc accccatagg
      121 cctgtacatg tttactcaga aatatacatc cttttctctg tcataaaccc tctgattaat
      181 cataaataaa tacttgtgac accaatcttt ttccttaacg gaacgaattg ttgtgtagaa
      241 ggagataata ttatgtcaaa aaaatatcag ccattgctta taactcatta tatgtcaaca
      301 tgggtcacta taacggaagc agttgagatc actaccaaag ccataaaaca aaaaattact
      361 cctagcgata tttatcgtca tgccttgagt ggcaacatcc tactatcgat ctatttccag
      421 tctcctgtga tacttaagaa gatacaaact tttaatggaa aaataaaatt caggcagttt
      481 gtgggagacc tacttgataa actatgcatg cttgacaggg acggttttat ttatggtcaa
      541 aatttaagac tctgtacaga agccagatat atttgtcctg tgcaacaaat tatagatacc
      601 cccctgttac gcaaactcaa ccagtttcgt acgtttgttc gtaatgtccg cccgggtgat
      661 gaactggatg tccaggcaca agttagtgaa aaaaatttaa ccccgccgcc gggtaacagc
      721 agtggcaacc ttgagcaaca gatagccagt acttcacagc taattggatc tctgctcgcc
      781 gaagatatga acagcgagca agcggcaaac atagcacgtg gatgggcctc ttctcaggct
      841 tcaggggtaa tgacagactg gttaagccgc ttcggtaccg caagaatcac gctgggcgtg
      901 gatgaagatt tcagcctgaa gaactcccgt gacggcaacc ctcgaaaatg gcgattcgat
      961 gcaacaaaca gtgaactatg tgccgaacgt cacgaatgct gaaatcacgc tggcagcctc
     1021 gaaggatccg gtgattgccg acaataacga tctcacgaca ctaacagcac cgtcgctgat
     1081 acagagggca atgcgatagc caacactgag gtaacattta ctctaccgga agatgtgaag
     1141 gcgaacttca cgctgagcga tggcgacaaa gcggttactg atgccgatgg caaagcgaaa
     1201 gtcacgctga aaggtacaaa agcaggcgct cataccgtga cagcatcgat ggttggcggt
     1261 aagagtgagc agttggtggt gaactttact gcggatacgc tcactgcgca ggttaatctt
     1321 aacgttaccg aggacaattt catcgccaat aacattggaa tgaccaggct acaagcaaca
     1381 gtgactgatg gaaacggcaa cccagtagaa ggtataaaag ttaatttccg tggaacctcc
     1441 gtcacgctaa gcagcaccag cgttgaaacg gatgatcagg tttttgctga aattcttgtg
     1501 acaagcaccg aggtcggact aaaaacagtt tcagcctctc tggcagataa acctactgaa
     1561 gtcatctcgc gattactgaa tgccaaggta gatgttaatt ctgcgacgat taccagtcag
     1621 gagatacctg aaggtcaggt catggtcgca caagacatag cagttaaagc tcacgttaac
     1681 gaccagtttg gcaacccggt gacgcatcaa cccgcgacat tcagtgcagc gccatcctcg
     1741 caaatgatca tcagccagaa cacggtctct actaatacgc agggagtagc cgaggtcacc
     1801 atgacgtctg aaagaaacgg ctcgtatact gtaaaagcat ccctagcgaa tggagcttca
     1861 cttgagaaac aactggaggc tattgatgaa aaactgacac tcacgtccag tccgcttatc
     1921 ggtgtcaatg ctcctaaagg cgctactctg acggcaacgc taacctctgc aaatggcact
     1981 ccagtggagg gtcaggtcat caactttagc gtaacgctag aaggtgcgac gttaagtggc
     2041 ggaaaagtga gaaccaactc ttcagatcag gctccagtcg ttctgaccag caataaagtc
     2101 ggtacatata cggtgactgc atcgttccat aacggcgtca caatacagac acagacaacc
     2161 gtgaaggtta ctggcaaccc aagcaccgca catgttgcta gctttatcgc tgatccgtcg
     2221 actatcgccg ccaccaacag tgatttaagt accttaaagg caacggttga ggatggcagt
     2281 ggtaacctga tcgaaggtct cactgtgtac ttcgccttaa aaagcggctc caccacatta
     2341 acgtcattaa cagccgtgac cgatcaaaac ggaatcgcga caacaaacgt gagatgagcg
     2401 ataacaggga gcgtcactgt aagcgcagtc ataagcgcag gtggaatgca aacagtagat
     2461 atatcgctag tggcaggccc agcagacgcc tcgcagtcca tccttaagaa caaccagtca
     2521 tcactgaaag gggactttac cgatagtgct gagctacatc ttgttctgca cgatatatca
     2581 ggcaatccga tcaaagtttc ttaagggata gaatttgtgc aatcaggtac aaacgtgccc
     2641 tatatgaaaa ttagcgcaat tgattacagt caaaatatca acggtgatta caaagctact
     2701 gttacaggag gcggagaggg tatcgcaacg ctgatccccg tattgaatgg tgttcatcag
     2761 gcgggtctga gtaccacgat agaattcatt agcgcggaaa ccagaccaat gacgggtaca
     2821 gtgtcagtga atagtgcaaa cctaccgaca gcttcattcc cttcgcaggg atttaccggg
     2881 gcgtattatc agttgaataa tgacaacttt gcaccaggaa aaacggcggc tgattattcg
     2941 ttttcaagct cggcctcctg ggtaggcgtt gatgctactg gtaaagtgac attcaaaaat
     3001 gacggtgata gcaatactgt aataatcaca gcgcccccaa gatccggtgg tgctatttac
     3061 caaacagttc ctcctgaatc gcgttcggtg tgactatgat cattttcggt gagtattgct
     3121 ttgatccaaa ggctggtttt ctgtgatgat tgaccagcct cagagtattg atgacaccac
     3181 cgttcataac agatcggcat tactatgcat aagtactgcc tcaaattctc gccaaaacac
     3241 tttttaatgc ctttgcgaat tcatccagac ttatattgtc aattttttgc ccaacgacga
     3301 ctgactccga aacatcggcc attgcgggcc atcgcgtttt gtaatcagca taaattgcaa
     3361 caacaggggt attataggca cttgccatat gtaatattga ggtgtcagga gttattgcaa
     3421 tataagcatc ttttatgatt gctgctgaac gtttaatcga gggtgataag gataaacggt
     3481 agacattatt ataacaatcc actaatgccc tggctttatc ttcccctttg ggtccataaa
     3541 cgatcactat tggaatatct gtttcacttt gaatttttgc tattagattt tctgcaatcg
     3601 ataatgaaaa tgtacgttcc tgcgagctac cttctaaatt gagcgcaatg taagatccta
     3661 acgatcgcat ttcctcgcgt acctcatcga gtacatcatc gcttaatggc aattcatata
     3721 ttgacctgac tgccggaaaa cccgcctcac gcatcataaa tgcccatgtc atgggtacag
     3781 gagcccggag attctgatcc atccgggacg cgttcttaca caaaggggaa tagcacttca
     3841 tggttaaacc gacgacctgg aaattcgttt ttgctttcaa ctgactgata aatatcatcg
     3901 ttttcaggtt ctttttacgc atcgcctcta tacaaagatc cggcgtacca tattgcagtg
     3961 ttatgttctt cgctaaatct tttatttctt ttaatgttgc gtgatcctgc atagtcattg
     4021 tgactaatgt taatttagtc tgttcaagtt tgagcgcatt aaagacttct aaattaattg
     4081 tcgacgtaac aattaataga tgcttaattt tatgcaattc aagcgcccga ataacaggaa
     4141 agatggccat agcatcgcca atctgatcgg g
//
LOCUS       DADSZD010000259         4135 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.261, whole genome shotgun sequence.
ACCESSION   DADSZD010000259 DADSZD010000000
VERSION     DADSZD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.261"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            140..1243
                     /gene="proB"
                     /locus_tag="J1Z28_004274"
     CDS             140..1243
                     /gene="proB"
                     /locus_tag="J1Z28_004274"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBA7719035.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1255..2508
                     /gene="proA"
                     /locus_tag="J1Z28_004275"
     CDS             1255..2508
                     /gene="proA"
                     /locus_tag="J1Z28_004275"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBA7719036.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2623..2698
                     /locus_tag="J1Z28_004276"
     tRNA            2623..2698
                     /locus_tag="J1Z28_004276"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2656..2658,aa:Thr,seq:cgt)
     gene            2919..3104
                     /locus_tag="J1Z28_004277"
     CDS             2919..3104
                     /locus_tag="J1Z28_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719037.1"
                     /translation="MASGLICHGGFEPHAWGVASLMDVTVLVVASLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            3319..3933
                     /locus_tag="J1Z28_004278"
     CDS             3319..3933
                     /locus_tag="J1Z28_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HBA7719038.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            4007..4135
                     /locus_tag="J1Z28_004279"
                     /pseudo
     CDS             4007..4135
                     /locus_tag="J1Z28_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354832.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT         1018 a          970 c         1110 g         1037 t
ORIGIN      
        1 cgtaaacaac tgccgctacg cttgctgatc ccgcgcaaca aaacgccatg ctttgcttgc
       61 agatggttgg caaccgacga cagtcctgct aaaacgttcg tttgatatca tttttcctaa
      121 aattgaatgg cagagaatca tgagtgacag ccagacgctg gtggtaaaac tcggcaccag
      181 tgtgctaaca ggcggatcgc gccgcctgaa ccgtgcccat atcgttgaac ttgttcgcca
      241 gtgcgcgcag ttacatgccg ccgggcatcg gattgttatt gtgacgtcgg gcgcgatcgc
      301 cgccggacgt gagcacctgg gttacccgga actgccagcg actatcgcct cgaaacaact
      361 gctggcggcg gtagggcaga gtcgactgat tcaactgtgg gaacagctgt tttcgattta
      421 tggcattcac gtcgggcaaa tgctgctgac ccgtgctgat atggaagatc gtgaacgctt
      481 cctgaacgcc cgcgacaccc tgcgtgcgtt gctcgataac aatatcgttc cggtaatcaa
      541 tgagaacgat gctgttgcta cggcagagat taaggtcggc gataacgaca atctttctgc
      601 gctggcggcg attctggcgg gtgccgataa actgttgctg ctgaccgatc aaaaaggttt
      661 gtataccgct gacccgcgca gcaatccgca ggcagaactg attaaagatg tttacggcat
      721 tgatgacgca ctgcgcgcga ttgccggtga cagcgtttca ggcctcggaa ctggcggcat
      781 gagtaccaaa ttgcaggccg cagacgtggc ttgccgtgcg ggtatcgaca ccattattgc
      841 cgcgggcagc aagccgggcg ttattggtga tgtgatggaa ggcatctccg tcggtacgtt
      901 gttccatgcc caggcgactc cgcttgaaaa ccgcaaacgc tggattttcg gtgcgccgcc
      961 ggcgggtgaa atcacggtag atgaaggggc aaccgccgcc attcttgaac gcggcagctc
     1021 cctgttgccg aaaggcatta aaagcgtgac tggcaacttc tcgcgtggtg aagtcatccg
     1081 catttgcaac ctcgaaggtc gcgatatcgc ccacggcgtc agtcgttaca acagcgatgc
     1141 attacgccgt attgccggac accactcgca agaaattgat gcaatactgg gatatgaata
     1201 cggcccggtt gccgttcacc gtgatgacat gattacccgt taaggagctg gccgatgctg
     1261 gaacaaatgg gcattgccgc gaagcaagcc tcttataaat tagcgcaact ctccagccgc
     1321 gaaaaaaatc gtgtgctgga aaaaatcgcc gatgaactgg aagcacaaag cgaaagcatc
     1381 ctcaacgcta acgcacagga tgttgcagac gcgcgtgcca atggccttag cgaagcgatg
     1441 cttgaccgtc tggcactgac gcccgcacgg ctgaaaggca ttgccgacga tgtgcgccag
     1501 gtgtgcaatc tcgccgatcc tgtggggcag gtaatcgatg gcggcgtact ggacagcggc
     1561 ctgcgtcttg agcgtcgtcg cgtgccgctg ggggtgattg gcgtgattta tgaagcgcgc
     1621 ccgaacgtga cggttgatgt tgcttccctg tgcctgaaaa ccggtaacgc agtgatcctg
     1681 cgcggcggca aagaaacctg tcgcactaac gctgcaacgg tagcggtgat tcaggacgcc
     1741 ctgaaatcct gcggcttacc ggcgggtgcc gtgcaggcaa ttgataatcc tgaccgtgcg
     1801 ctggtcagtg aaatgctgcg tatggataaa tacatcgaca tgctgatccc gcgcggtggg
     1861 gctggtttgc ataaactgtg ccgcgagcag tcgacaatcc cggtgatcac aggcggtata
     1921 ggcgtatgcc atatttacgt tgatgaaagc gcagagatcg ctgaagcatt aaaagtgatc
     1981 gtcaacgcga aaactcagcg accgagcaca tgtaatacgg tagaaacgtt gctggtaaat
     2041 aaaaacatcg cagatagctt cctgcccgca ttaagcaaac aaatggcgga aagcggcgtg
     2101 acgttacacg ctgatgcagc cgcgctggcg cagttgcagg caggccccgc gaaggtggtg
     2161 gctgttaaag ccgaagagta tgacgatgag tttctgtcat tagatttgaa cgtcaaaatc
     2221 gttagcgatc tggatgacgc cattgcccat attcgtgaac acggcacgca acactccgat
     2281 gcgatcctga cccgcgatat gcgcaacgcc cagcgttttg ttaacgaagt ggattcctcc
     2341 gctgtttacg ttaacgcctc tacgcgtttt accgacggcg gccagtttgg actgggggcg
     2401 gaagttgcgg taagcacaca aaaactccac gcgcgcggcc caatggggct ggaagctctg
     2461 accacttata agtggatcgg cattggtgat tacaccattc gtgcgtaaat aaaaccgggt
     2521 gatgcaaaag tagccatttg attcacaagg ccattgacgc atcgcccggt tagttttaac
     2581 cttgtccacc gtgattcacg ttcgtgaaca tgtcctttca gggccgatat agctcagttg
     2641 gtagagcagc gcattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt
     2701 taaatcaata agttacacat cactaatacc ttccttattt tttattggga cagatttggg
     2761 accgatgggt tcaggatcga atctatttac cgtgcgtgtt cggtaaggtg attaggtgcg
     2821 aggtgagcat atcgacgaac catttcgaga gtttcccatc cgctcatttt ttagtgcaag
     2881 cagggaaaca ccggactaaa ccaatcagct gttcgaaaat ggcaagcgga ttaatatgcc
     2941 atggcggctt tgagccgcac gcatggggtg tggcaagcct gatggatgtt actgtgcttg
     3001 tggtggcgtc tttgctggtc tggttgttgg ttaatcgcga aaatcattat gccagaggaa
     3061 taccacagga cgttcaggtt ttgcataaaa aagcccacca ttaaggtggg caaaaggaat
     3121 gatttatatt ctctgagaga aacattacat ttctccttgt cacttctaac atgaaaatat
     3181 ggagaaataa tggagcttat attaattggc gattaggtaa ccatgtggtt atacaaaaca
     3241 atacagttct ttacatttgc cttgttttat gaatactcct aaagaggtgt ataacataat
     3301 ggtacaagca gggtagatat gaatatattt gaacaaactc caccaaaccg cagacgttat
     3361 ggtcttgctg cattcattgg gctgattgct ggcgttgttt ccgcatttgt taagtggggg
     3421 gcagaagttc cattgccacc acgtagtccg gttgatatgt ttaatgcagc gtgtggtccg
     3481 gaatcattaa tcagggctgc aggccaaatt gattgctccc gtaactttct caatcctccg
     3541 tacatttttc ttcgggattg gttgggactg acagatccaa attcggctgt ttataccttc
     3601 gccgggcatg tctttaactg ggttggtgta acgcacataa tcttttcgat agtttttgcc
     3661 gttggttact gtgtggtcgc cgaagtattt ccgaaaatta aactttggca gggcttactg
     3721 gcaggtgctt tagcgcaact ttttgttcat atgatttcat tccctctcat gggacttacg
     3781 ccacctctct ttgatcttcc gtggtatgag aatgtctctg aaatttttgg acatttagtc
     3841 tggttctggt ctattgaaat tattcgcaga gatttacgaa acagaattac ccatgagcca
     3901 gatcctgaga tccctttagg ctcaaataga taatgctttg aatgataaaa atggcgcaaa
     3961 tatagcgcca tttttatagg ttaaaaacgt tgcttttcat attctgatgc ggacagtcag
     4021 tgatcatatc gcgctacttc aggatgatgt agatccggag aacgctacag aaaaagaggc
     4081 attgttgctg gaaaatggaa gaagtttcag gttttgttgt ccctgaaaaa cgaag
//
LOCUS       DADSZD010000260         3982 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.262, whole genome shotgun sequence.
ACCESSION   DADSZD010000260 DADSZD010000000
VERSION     DADSZD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.262"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            396..602
                     /locus_tag="J1Z28_004280"
     CDS             396..602
                     /locus_tag="J1Z28_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HBA7719039.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            678..974
                     /locus_tag="J1Z28_004281"
     CDS             678..974
                     /locus_tag="J1Z28_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBA7719040.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            980..1765
                     /gene="bet"
                     /locus_tag="J1Z28_004282"
     CDS             980..1765
                     /gene="bet"
                     /locus_tag="J1Z28_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HBA7719041.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     gene            1762..2442
                     /locus_tag="J1Z28_004283"
     CDS             1762..2442
                     /locus_tag="J1Z28_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HBA7719042.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFSNYDPRMKREGLHYVVVERDEKYIASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            2439..2597
                     /locus_tag="J1Z28_004284"
     CDS             2439..2597
                     /locus_tag="J1Z28_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HBA7719043.1"
                     /translation="MKHPHDNIRVGVIAFVHSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAV"
     gene            2594..3151
                     /locus_tag="J1Z28_004285"
     CDS             2594..3151
                     /locus_tag="J1Z28_004285"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017693151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBA7719044.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAANEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            3162..3443
                     /locus_tag="J1Z28_004286"
     CDS             3162..3443
                     /locus_tag="J1Z28_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719045.1"
                     /translation="MHFSGSGLHILCAYACRHGTCSMTPQQENALRSIARQANSEIKK
                     ARQQFPDKNVDDICRSVLKKHRETVTLMGFTPTHLSLAIGMLNGAFKER"
     gene            3542..3763
                     /locus_tag="J1Z28_004287"
     CDS             3542..3763
                     /locus_tag="J1Z28_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HBA7719046.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHLAVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQQDLELIRKQRGIK"
     gene            3763..>3982
                     /locus_tag="J1Z28_004288"
     CDS             3763..>3982
                     /locus_tag="J1Z28_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBA7719047.1"
                     /translation="MANLQLAVKSEYFDSMIRGEKTEEYRLCNDYWNKRIMFREYDRL
                     IITKGYPKRDDSSRRIDVPYDGYEVKTIT"
BASE COUNT         1084 a          973 c         1009 g          916 t
ORIGIN      
        1 gttatcgcta tgcgatgcaa gtcacaaaat aaatccatcc taaatacaac cagttatatc
       61 taaaacaacc aatgaaacaa cttttgttgt tgacggcaaa acaactatag ttttaaatga
      121 gttcatcgca acaacacaac gatacggcaa ccacctgatt caccgttgcg atgaccgctt
      181 agatccgcag cttgaatttc agcaggctcc ggggagtgcg aggggtgaaa cggacgtgtg
      241 aacgtcggtg tgaccagctg aaatcaactc aacacctcat acctcagtcg cttcaacgag
      301 gcggcttagt tatgacaacc ggcggccatc caccgcctga atacgcgcag aagtctctat
      361 atgttcagca gcccagctta cgggcaggag tttttatggt tcatcaacat tacggaacgc
      421 agaccgttaa tcgaggtgcg gtcatgccag gaatgctggt caaacacaaa gatggtacct
      481 ggactgcatc agctaattta cgcggacggc tttatctgca tcgcggcatc gagcgcactt
      541 atacccgtga tttgctcgtg gaagtttttc tcgacggacg cggtaacggc ctgaatcact
      601 aatccccttt cctgttttcc taatcagcct ggcatttagc gggcgatatt ttcacagcca
      661 ttttcaggag ttcagccatg aacgcttatt acattcagga tcgtcttgag gctcaaagct
      721 gggcgcgtca ctaccagcag atcgcccgtg aagagaaaga ggcagaactg gcagacgaca
      781 tggaaaaagg cctgccccag cacctgtttg aatcgctatg catcgatcat ttgcaacgcc
      841 acggggccag caaaaaagcc attacccgtg cgtttgatga cgatgttgag tttcaggagc
      901 gcatggcaga acacatccgg tacatggttg aaaccattgc tcaccatcag gttgatattg
      961 attcagaggt ataaaacgga tgagtacagc actcgcaacg ctggcaggga agctggctga
     1021 acgtgtcggc atggattctg tcgacccaca ggaactgatc accactcttc gccagacagc
     1081 atttaaaggc gatgccagcg atgcgcagtt catcgcattg ctgatcgtcg ccaaccaata
     1141 cggccttaat ccgtggacga aagaaattta cgccttccct gataagcaga acggcatcgt
     1201 tccggtggtg ggcgttgatg gctggtcccg catcatcaac gaaaaccagc agtttgacgg
     1261 catggacttt gaacaggaca atgaatcctg tacatgccgg atttaccgca aggaccgtaa
     1321 tcatccgatc tgcgttaccg aatggatgga tgaatgccgc cgcgaaccat tcaaaactcg
     1381 cgaaggcaga gaaatcacgg ggccgtggca gtcgcatccc aaacgaatgt tacggcataa
     1441 agccatgatt cagtgtgccc gtctggcctt cggatttgct ggtatctatg acaaggatga
     1501 agccgagcgc attgtcgaaa atactgcata cactgcagaa cgccagccgg aacgcgacat
     1561 cactccggtt aacgatgaaa ccatgcagga gattaacact ctgctgatcg ccctggataa
     1621 aacatgggat gacgacttat tgccactctg ttcccagata tttcgccgcg acattcgtgc
     1681 atcgtcagaa ctgacacagg ccgaagcagt aaaagctctt ggattcctga aacagaaagc
     1741 cgcagagcag aaggtggcag catgacaccg gacattatcc tgcagcgtac cgggatcgat
     1801 gtgagagctg tcgaacaggg ggatgatgcg tggcacaaat tacggctcgg cgtcatcacc
     1861 gcttcagaag ttcacaacgt gatagcaaaa ccccgctccg gaaagaagtg gcctgacatg
     1921 aaaatgtcct acttccacac cctgctggct gaggtttgca ccggtgtggc tccggaagtt
     1981 aatgctaagg cgctggcctg gggaaaacag tacgagaacg acgccagaac cctgtttgaa
     2041 ttcacttccg gcgtgaatgt tactgaatcc ccgatcatct atcgcgacga aagtatgcgc
     2101 accgcctgct ctcccgatgg tttatgcagt gacggcaatg gccttgagct gaaatgcccg
     2161 tttacctccc gggatttcat gaagtttcgg ctcggtggtt tcgaggccat aaagtcggct
     2221 tacatggccc aggtgcagta cagcatgtgg gtgacacgaa aagatgcctg gtacttttcc
     2281 aactatgacc cgcgtatgaa gcgtgaaggc ctgcattatg tcgtggttga gcgggatgaa
     2341 aagtacatag cgagttttga cgagatggtg ccggagttca tcgaaaaaat ggacgaggca
     2401 ctggctgaaa ttggttttgt atttggggag caatggcgat gaagcatcct cacgataata
     2461 tccgggtagg cgtgatcgct ttcgtccact ccgttacgaa gcgaggctgg gtatttcccg
     2521 gcctttctgt tatcagaaat ccactgaaag cgcagcggct ggctgaggag ataaataata
     2581 aacggggggc tgtatgactg atttcaccgg aagcaatact cctgccgaac atcgcgacag
     2641 ctggcgcaca ccaccagaga tttttgctgc gctgaatgca gagttcgttt ttcaacttga
     2701 tgctgcagcc aacgaaaaaa accggctatg tcggcttttt atctcacagg agcagaacac
     2761 attaaccact tcatggcctg aagcaatggg atatgcctct ggttatgtct ggctgaatcc
     2821 accatacagc aatatttccc cttttgtgaa aaaggcagcc accgaaaaca aattcagtag
     2881 tgtgggatgt gtaatgttat tgcctgctga cacgtctgtc ggatggtttc atgaagcgat
     2941 acaaaccgcc agtgaggtca gattcatcac agcaggacga ctggcattta ttaacccact
     3001 caccgagaaa cccgtgagtg gaaataataa aggctcgatg ctcattatct ggcacccata
     3061 cccccgtaca cactgccact ttacgaccgt tgatcgtggc gagttgatgg cgttcggctc
     3121 aaggattctt gcccgtcggg aggctgcatg acaaccacgg aatgcatttt tctggcagcg
     3181 ggcttcatat tctgtgtgct tatgcttgcc gacatgggac ttgttcaatg acacctcagc
     3241 aagaaaacgc ccttcgcagt attgcccgtc aggctaattc tgaaatcaaa aaagccagac
     3301 agcagtttcc ggataaaaac gtcgatgaca tttgccgtag cgtactgaag aagcaccgcg
     3361 aaacggtaac gctgatggga ttcacaccga ctcatttaag tctggcaatc ggtatgttaa
     3421 acggcgcctt taaggaacgg tgaacatgaa aagcaaaatc ctcagggagc tacaggctcc
     3481 ttttttattg ttcgcattca ccctcaagcg tattaaccaa caattcaggg attaatggaa
     3541 gatggcagac atcattgatt cagcatcaga aatagaagaa ttacagcgca acacagcaat
     3601 aaaaatgcgc cgcctgaacc acctggctgt atctgccact cattgttgtg agtgtggcga
     3661 tccgatagat gaacgaagac gactggccgt tcagggttgt cggacttgcg ccagttgcca
     3721 gcaagatctg gagcttatcc gcaaacaaag ggggattaag tgatggctaa cctgcaactt
     3781 gctgttaaaa gtgaatactt cgattccatg attcgcggag agaaaacgga agagtatcgc
     3841 ttgtgtaatg actactggaa taagcgaatt atgttccggg aatatgaccg cctgattatc
     3901 acaaagggat atccgaagcg cgacgattcc agtcgcagaa ttgatgttcc gtatgacgga
     3961 tatgaagtga agacaatcac ac
//
LOCUS       DADSZD010000261         3954 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.263, whole genome shotgun sequence.
ACCESSION   DADSZD010000261 DADSZD010000000
VERSION     DADSZD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.263"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..747)
                     /locus_tag="J1Z28_004289"
     CDS             complement(<1..747)
                     /locus_tag="J1Z28_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN/FAD transporter"
                     /protein_id="HBA7719048.1"
                     /translation="MLRHILTAKNILSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSSFEMWFHFLQLRSALNISSALRQVVHGTRWHSKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTDVKALALTYLEL
                     TVFSYPAAAITLIGSGALRGAGNTKIPLLIN"
     gene            752..827
                     /locus_tag="J1Z28_004290"
     tRNA            752..827
                     /locus_tag="J1Z28_004290"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:785..787,aa:Asn,seq:gtt)
     gene            complement(865..1815)
                     /gene="cbl"
                     /locus_tag="J1Z28_004291"
     CDS             complement(865..1815)
                     /gene="cbl"
                     /locus_tag="J1Z28_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBA7719049.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1917..2834)
                     /gene="nac"
                     /locus_tag="J1Z28_004292"
     CDS             complement(1917..2834)
                     /gene="nac"
                     /locus_tag="J1Z28_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator"
                     /protein_id="HBA7719050.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3161..3236
                     /locus_tag="J1Z28_004293"
     tRNA            3161..3236
                     /locus_tag="J1Z28_004293"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3194..3196,aa:Asn,seq:gtt)
     gene            complement(3295..3855)
                     /locus_tag="J1Z28_004294"
     CDS             complement(3295..3855)
                     /locus_tag="J1Z28_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA7719051.1"
                     /translation="MSVQENTINILHELENNALKIDDSQAAQFISQIRNARHIFLQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEISSPHTQPGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDDNNRVAGTFAQPMGSAFEQLCFITY
                     DAIVLALMSELGESSATMFTRHADLE"
     gene            complement(3871..>3954)
                     /locus_tag="J1Z28_004295"
     CDS             complement(3871..>3954)
                     /locus_tag="J1Z28_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HBA7719052.1"
                     /translation="LAIFLADKDNSYIQGQTILMDGGRTIA"
BASE COUNT         1099 a          996 c          841 g         1018 t
ORIGIN      
        1 gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc taccaataag
       61 agtgatggca gctgctggat aactgaatac ggtcagctcc agataagtca acgccagtgc
      121 tttaacgtct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc caaaatgatg
      181 aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact gccgcgtcgc
      241 caccctcgct cgtcgtcgat ctcgcttacc gagactaaat gctacaacga cagtagtacc
      301 aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg ccaaccccac
      361 gccggccatc gcatcttttc ccagccagct gaccagaaaa gtgctcagga cccccatcaa
      421 caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct cgcgccagaa
      481 caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa cctggcgtaa
      541 agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca tttcaaatga
      601 tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc aaatgcaaca
      661 aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg acaaaatatt
      721 tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg tagaacggcg
      781 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat ttctgaaaag
      841 cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt cactgttttc
      901 catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaagtt ccagaaagcg
      961 ccagacatag ttacgttgaa gttgccctcg cttcagtccc aaccagacag tattagcatc
     1021 gaaaagatgc ctcgtatcca gacggattaa attcttttcc tcttgttcac cactggattg
     1081 ttcggcaact aatccgatcc ccagcccaag agcaacatag gttttaatga catcagaatc
     1141 ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gcaaatgcgt catcaatgcg
     1201 tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta ttgattccag
     1261 cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac tatggtgcca
     1321 gcgaaaccac gggaaggcga cgagctgcgg gtcattactc aaacgctcgc tggcgatacc
     1381 aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac cctggattag
     1441 ctcgagccta acctctggga aaagttcgcg aaaagcttta atgacctctg gcaagctata
     1501 acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt tggtaaacag
     1561 gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa tgaccagtaa
     1621 tgctttgccc ggttcagtca tgcccagcag tcgcttacct cgtcgaacaa atatttcgat
     1681 gccaagttca tcctccagtt cccgaatatg acggctaacg cctgactgtg aggtaaaaag
     1741 catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga ttatctttag
     1801 ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg ttaaagtcta
     1861 atgcccggca taacaaataa taaaaacccg catcttattc catcccgata taacgcttag
     1921 ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga cattaacaac
     1981 tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt taaggacaaa
     2041 gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc gccacataac
     2101 gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat cgctgcggta
     2161 agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg tagggaaaaa
     2221 gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag gttcatttgc
     2281 gcaatagcat taacatcaac gctttgcccc gggcaatctt gagttcctac caggaaaaga
     2341 tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc ataaatcacc
     2401 gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact attttcatga
     2461 agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg catggtgatg
     2521 gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa tgactgacca
     2581 acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg tgcatgggta
     2641 tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat caaaagctgt
     2701 tgatttaact caccttccag tgtggcaacc tgctggctga gcgctggttg tgcgatatgc
     2761 aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa gtatttcagg
     2821 cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg ccattactta
     2881 caaccagaat gcaagatgct tgccagttct attttggcgt tgatgcgcaa gctaaccaac
     2941 tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca gtcgcattca
     3001 cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa gattgaaagc
     3061 ccaccacttt gttgaaattg gcagcaaaca aacaaaaaaa tgcatttcac cctttgacat
     3121 caccatgcac tgccattaat atgcgccccg ttcacacgat tcctctgtag ttcagtcggt
     3181 agaacggcgg actgttaatc cgtatgtcac tggttcgagt ccagtcagag gagccaaatt
     3241 caaaaaagcc tgctttcgag caggcttttt gctttctaat taccaactta ttggtcattc
     3301 cagatcagca tgtcgggtaa acattgtggc actgctttca cccagttccg acatcaacgc
     3361 cagaacaatt gcatcataag taataaagca gagttgctca aaagctgagc ccattggttg
     3421 cgcgaaagtg cctgcaacac gattgttatc gtctttcaca cttcctggta gtaccaacac
     3481 agattgagct aatttaccga tcgtcgaatc agctttcata gtgaccagag cgacgtttac
     3541 tccgctatca actgcttttt gtgccagact tttcaaactg gtagtttcac cactgcctga
     3601 cccaataatt aatagatctc caggttgggt atgcggtgat gatatttctc ccacaacgct
     3661 tactgaaaaa cctaagtgca gcaagcgatt agcaaaagcg cgaatagcaa tgccactacg
     3721 cccagctccc tgcaaaaaaa tatgccgggc atttctgatt tgcgaaataa actgtgcagc
     3781 ctgagaatcg tcaatcttca gcgcattatt ctctaattca tgcagaatat taatagtatt
     3841 ttcctgaaca ctcatttttt ctctcccttt ttaagcaata gtccggccac catccattaa
     3901 aatggtttgt ccctgaatat aagagttatc tttatccgcc aaaaatattg ccag
//
LOCUS       DADSZD010000262         3952 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.264, whole genome shotgun sequence.
ACCESSION   DADSZD010000262 DADSZD010000000
VERSION     DADSZD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.264"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..530)
                     /locus_tag="J1Z28_004296"
     CDS             complement(<1..530)
                     /locus_tag="J1Z28_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBA7719053.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFILLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIP"
     gene            complement(531..2030)
                     /locus_tag="J1Z28_004297"
     CDS             complement(531..2030)
                     /locus_tag="J1Z28_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBA7719054.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(2091..2981)
                     /locus_tag="J1Z28_004298"
     CDS             complement(2091..2981)
                     /locus_tag="J1Z28_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBA7719055.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYEIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDH
                     LNGKPVEKMIPVQPGVYTGK"
     gene            complement(3017..3871)
                     /locus_tag="J1Z28_004299"
     CDS             complement(3017..3871)
                     /locus_tag="J1Z28_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HBA7719056.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDCVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
BASE COUNT         1092 a          979 c          791 g         1090 t
ORIGIN      
        1 ggtatataga gaaattctcc attccccagc gccagaaagg catcatcttt aatcatgatg
       61 ggcataccat cggtcactat ataagcgata ccacggaaga tccccatagt agcaacggtg
      121 acaataaacg acgggatccc cgctatggcc gtcaagccac catttatcat ccccagagcc
      181 agtgctgcaa taatggataa tgggaccaat aaaataaatg gaattcccca tgaagcccca
      241 agcgcagcaa atgtgccaat caatgcaatg gttgaaccaa ccgataaatc aatatcgcca
      301 agcaaaagta cgtaagtcat tccataggcg gtaattgcaa ttatcgaaac ctgaagaata
      361 atattagtaa tgttattggc ggtaaaaaaa ctgtcattaa taaaactgaa cagaatgatt
      421 aacaccacca ttccactgag gataccacca tattgcctga agactttttt aaaattattt
      481 gcaatgggaa tttccgaagg tttcggactg gcaagagttt tctctgtcat ttaacgtact
      541 cctgttgcag cggtcattac ctgtgtctgc gttaaatttt cacatgaata aatatcggca
      601 atcgttttat tacgcattac caccagacga tcgctaagtg ccatgacttc tggcagttca
      661 gaggacacca gaataattgc tgtccctgcc gcagccagtt gacgtatcac gttatagatt
      721 tcaaattttg cgccgacatc cactccgcgc gttggctcat cgagcaataa aatacgcgga
      781 gttttctcca gccacttcga taacacgatt ttctgttgat taccgccgct cagtgctccc
      841 actgcctgat gcacagaact cactttagtg ttcattctgg caattgattg cagcgtcttc
      901 gcctcttctt gtcggtgatt gatgagtccc atccgcgaag cgttatattg aaacgccaca
      961 tggtgcgtgt tcatattgac gtcgtgcatc agcactaacc cttcttcctt gcggttttcg
     1021 gtcacaaaag caatgccatg atcaatagcc tgtttgggat tagcgatcct gacttcctta
     1081 ccatcaatca acacagtccc gtgatacgaa acgaggccaa atagcgcctt catcacatcg
     1141 gaacgcccgg cccccaccag gccaaaaaat ccgaggactt cccccggacg cacagcgaaa
     1201 ctgacctctt taaaaagcga aggatgggaa agatttttta cttccagttt tgcaggaaca
     1261 ttttcatccg gtttttcccc aagacgtgct ggccaaatat ttttcatttc ccgaccaacc
     1321 atttgggcaa tcaagtgctg gatatcactg tttttctttt catgcgtagc gatatactca
     1381 ccgtcgcgga tcaccgaaat atcatctgaa atccgcataa tttcatccat gcgatgagag
     1441 atataaataa ccgcacagcc tcgctgcttt aatttttcaa taatgttaaa caggatctcc
     1501 gcttcattat cacttagcga agatgtcggt tcatccataa tcacgacatc cgctgcatag
     1561 cttaaggctt tagcaatttc taccagttgc ctatgtgcca tcgacatatt gccgaccatt
     1621 tccagagggt ctagtgggat gcccagttcc gcgaggaggt tctggcaatc tgcgagcatt
     1681 tttttatcgt taatgatgcc aaaacgtctc ggttcattat tggcataaat attttccgcc
     1741 acggtcatat tattactcag gctcagttcc tgaaaaataa tcgcaatccc taactggcga
     1801 gaatgctgcg gattattaat ggtgatggtt tcaccacgta gtgttatcgt tccctgggtt
     1861 ggcggataaa caccggccag tattttcatt aatgtcgatt taccggcacc attctcgcct
     1921 aataaagtat gcacccgccc tttacgtaaa gagagtgtaa tatcttttaa tgcctgaaca
     1981 tgaccaaatg tcttcgcaat atgcgaaaca tcaagaataa catccgtcat aatgacctcg
     2041 gtgtcacaac aaaacgccaa taacgttttg ttgtgaggtg aagatattta ctatttacct
     2101 gtataaacgc cgggttgtac cgggatcatt ttttctaccg gtttaccatt gaggtgatca
     2161 acagcggcat caatggcttt agcacccatt tgatcgggat attgccgaat cacggccgca
     2221 aacgtttttt cactgtcaac ggcatcacgc gcttctttca tgccgtcaaa accaatgatt
     2281 ttgatgttgt cgttatgcgc agattttgct gcgataaccg ctgccagcgc cgcatcatca
     2341 ccaaagccaa acagaccatt caggttagca tttgcctgca taatgttttg cgcagtcgtt
     2401 aacgcttcag cccgcgtaat accaggctgt tcagcaacaa ttttaatatc cggtgtatcg
     2461 cttaaccctt tcttaaaacc atcaacgcgc gcaaccaccg attgtagtgc cggataagaa
     2521 ataatggcta cagtaccttt accatgcaaa agtttaccca ttaacttgcc tgcttcaacg
     2581 cctcccgcat agttatcggt cgcaacatgc gatacgaccg gaacgccttc cgcagcgacg
     2641 tcaacggtga taacgggaat accggcctta tcggctttaa ttatcgctgc ctggacgcct
     2701 ttcgaatcga ccggagagat aataatggca tccacttttt tagtaataaa atcctcaaca
     2761 tcagaaagtt gtttgtttaa atcctgattg gcaatagaga cacttaaatt gacattgtct
     2821 ttttttgcct cttctttcat ggcattcgca agatcaatat agaaaggatg ttgttgtgtt
     2881 aataatgacg caccaatttc atactctttt gccaacgaac tttgcgagaa caatgccagg
     2941 ctcaccaaca tagctgaacc aagtaattta atatttttca taaacaatcc tcattatttt
     3001 tgtagggtac aacgtcttaa atttttcctt cactaccaca gacgccaata atacgagtta
     3061 cgacttcttt catggcggcc tttgcgggct gcatatagtg acgtggatca tttgccgcag
     3121 gattgttgta gaaatatcct tttaacgcat cggcaaacgc aattttcaat tctgtcgcga
     3181 cattcacttt acaaataccg cgacgaatac agtgctgaac gtcctgttcc gataagcccg
     3241 aagcaccatg caacaccagc ggaatctcca ctcgctgacg aatctgttcc agccgcacac
     3301 aatccagtcg tggttcatgc gcatataaac catgtgctgt gccgattgct accgccaggg
     3361 agtcaatttg ggtacgttga acaaactctg cagctgcggc tggtgaggta taagcggaat
     3421 ctttttcatc cacccgcaga tcgtcctcct ggccgccaag acgccctagc tcggcctcaa
     3481 cgctaacatc aaagcgatgg caaaaggtgg taacttcttg cactcgcgca atgttgtctt
     3541 caaatggcaa atgggagcca tcaatcatga cagaacggat ccctgcgcga accttgttaa
     3601 aaatgtcatc actctcttca tgatggtcga gatgcaggac taacggcagg ttgtactcgc
     3661 gagccagcga ttgtactaac gaaattaaac ttcccacgcc accataacga tatgtaccgg
     3721 gtgttcccgc cagcataacg ggagagcgaa gttgtgcagc gctctcaaca actacctgta
     3781 aagtttcaag gttatgaatg ttgaaagcag ggacggcata tccctctgac tgggcttttt
     3841 tcagcatgtt tttgcttgaa ataatataca tcgcagctcc tgttattggt cgttcgcctt
     3901 acaaaatgaa gaaagagaat ctttactttc attttgaaag caaactagca ca
//
LOCUS       DADSZD010000263         3923 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.265, whole genome shotgun sequence.
ACCESSION   DADSZD010000263 DADSZD010000000
VERSION     DADSZD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.265"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            612..860
                     /locus_tag="J1Z28_004300"
     CDS             612..860
                     /locus_tag="J1Z28_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HBA7719057.1"
                     /translation="MFGKVEDKAKEVAGEVQEKYGELTDNCGHQVKGAARKIAAQGGQ
                     AAQEAADVVRNNVENNPFAAISIAAGVGLLIGLLIGRK"
     gene            932..1285
                     /locus_tag="J1Z28_004301"
     CDS             932..1285
                     /locus_tag="J1Z28_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719058.1"
                     /translation="MNAFKTLFLSALLVSTGVLADSSTHTGSLKNQASQQAEKISNRA
                     EEAAHKAEHRIEEKQQQAENRLRNGGEVSTTDKVKEDAQKAWNRTKETTEKAWNRTKE
                     AAQQAGDAVSEKVSE"
     gene            1369..1770
                     /locus_tag="J1Z28_004302"
     CDS             1369..1770
                     /locus_tag="J1Z28_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719059.1"
                     /translation="MRTGNIQPRGPSRSLVDNIHRVFNTLTGIFHTRIELIAIELEEE
                     KKNILGLLLMVGLALIFTVFGVVSLLIFILLSVSPEHRLIISGGASAVFLVIALFFVI
                     FIKQKTAGSRILSETRKQLSEDLNCLKRDDE"
     gene            1767..2060
                     /locus_tag="J1Z28_004303"
     CDS             1767..2060
                     /locus_tag="J1Z28_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719060.1"
                     /translation="MNTRKTEENRKQKLLKQIEQQRQTLGLLKADWLRITSPVDRGWQ
                     SLYQWRNVIVPAVGVAALYTLKSRPRRLIVWPRRVLAVWGGLRFIRRPFTSLR"
     gene            2405..>3923
                     /locus_tag="J1Z28_004304"
     CDS             2405..>3923
                     /locus_tag="J1Z28_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HBA7719061.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNTDKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNLSARTNEQKHANDALNALLKEKENIRNQLAGINQKIAEEKRKQDELKATK
                     DAINFTTEFLKSVSEKYGAKAEQLAREMAGQAKGKKIRNVEEALKTYEKYRADINK"
BASE COUNT         1197 a          790 c          977 g          959 t
ORIGIN      
        1 ttaaattata tcaataaggt tatttctgag ttatcgcggt tttatactga tgaaatagac
       61 tatgagtaat cttcagaggc atgttaatct cagggatatt gttttctgca ggaggcggat
      121 atacaggcct gtgcatttta ccgattattt aagtacacaa tttactgttc ctgtgtcggc
      181 atcagtggtt agttcactac attcgaacag aactgcctgc taagagttgg agttgatggt
      241 tatctattaa cagatatctt tagatggctg tctgtataac ttctggagta tttattgatg
      301 gcgattgctt gttaaacctg tgagataacc atttcatgtt cctccattga gaccataagg
      361 acagtgtact acgtgagttc tgtgattgtg cagggaactg ttggaagtga gcatttaatg
      421 aaaatgccac ggctttgctg atatagactg gtgagtatca ggtgaataaa aacaacctac
      481 aatcatattg ttgaatttaa tatttccctc tttacgtgag gaggatacat ggctcgtgcg
      541 ttgctaaata ccagtggacg atataaatgt atcggattaa atcttagttc ataatgcaga
      601 aggagtatat tatgtttggg aaagttgaag ataaagccaa agaagtagcc ggagaagtgc
      661 aggagaaata tggtgaactg accgataatt gcggacatca ggtaaaaggt gctgcccgta
      721 aaatcgctgc acagggtggt caggctgccc aggaggcggc ggatgttgta cgtaataatg
      781 tagaaaataa tcccttcgcg gcaatttcca tcgccgcggg tgttggcctg ctgattggtc
      841 tgctcatcgg acgaaaataa tatcagatgc gggcgaacag cccgcatttt tgtgccagat
      901 aatattgttt catctgtata aggagtaaag tatgaatgca ttcaaaacat tgtttctttc
      961 tgccctgctg gtatcaacag gcgtactggc tgattcgtcc actcataccg ggagcttaaa
     1021 aaaccaggcc agtcagcagg ccgaaaaaat atctaaccgg gcagaagaag cagcgcacaa
     1081 ggctgaacat cgtattgagg aaaaacaaca gcaggcggag aaccgtctca ggaatggtgg
     1141 agaggtgagt acaactgata aggtaaagga agatgcacag aaagcctgga acagaaccaa
     1201 agaaacaacc gaaaaagcct ggaacaggac taaagaagcg gcacagcagg ccggagatgc
     1261 tgtaagtgaa aaggtgagcg aataattctc cttccccggt cagaccgggg aaaatggagc
     1321 atgcagcgtg ctcagcagaa caatgattaa acaaaaaagg atgatgtctt gagaacagga
     1381 aatatccagc cacgtggacc atcccgtagt ctggtagaca atatacaccg tgtcttcaac
     1441 acccttacag gaatatttca tacccgcatt gagcttattg caattgagct ggaagaagaa
     1501 aagaaaaata ttctggggtt gctgctgatg gtggggttag ctctgatatt cactgtattt
     1561 ggggttgtat cgcttctgat attcattttg ctgtcagtct caccggagca tcggctgatt
     1621 atatcagggg gtgcttctgc tgtttttctg gttattgcat tattttttgt gattttcatt
     1681 aaacaaaaga ctgccggaag caggatacta tcagagaccc gtaaacaact cagtgaagac
     1741 ctgaactgtc tgaaaaggga tgatgaatga atacccggaa aacagaggag aaccgaaaac
     1801 agaaactgtt aaaacagatt gagcaacaac gacagacact gggcctgtta aaggcagact
     1861 ggctcaggat aacgtcgcct gttgaccgcg gctggcagtc attatatcag tggcgtaatg
     1921 ttatcgttcc cgcagttgga gtggctgcat tgtatacgct gaaatccaga cctcgtcgtc
     1981 tgattgtctg gccacgcaga gtactggcag tctggggagg gctaagattt atcaggcggc
     2041 ccttcacgtc gctccgatag agtaatttat atcgtattca gagaggcgtt gttctggcag
     2101 tgttactccg ttgacggaaa aacgttcagt tcttttctga cagtggctga gtgcaaaaac
     2161 attgttgctg ttatggggta tgtccggttt ttgtatttct cctgagaata ctcagactgt
     2221 gatgctgcca cagcgttagc aggctttccg aacggtataa ctcccccttc atcatgttaa
     2281 tgataatcac tatcattaaa tcttgacatg ccattttctc cttaataaat taatactgta
     2341 tatgcatcca tgtatacaag cagttatttc atttgttttc ctcagcggat gaaggagata
     2401 ccgaatgtct gaccctgtac gtattacaaa tcccggtgca gaatcgctgg ggtatgattc
     2461 agatggccat gaaattatgg ccgttgatat ttatgtaaac cctccacgtg tcgatgtctt
     2521 tcatggtacc ccgcctgcat ggagttcctt cgggaacaaa accatctggg gcggaaacga
     2581 gtgggtcgat gattccccaa cccgaagtga tatcgaaaaa agggacaagg aaatcacagc
     2641 gtacaaaaac acgctcagcg cgcagcagaa agaaaatgag aataagcgta ctgaagccgg
     2701 aaaacgcctc tctgcggcga ttgctgcaag ggaaaaagat gaaaacacac tgaaaacact
     2761 ccgtgccgga aacgcagatg ccgctgatat tacacgacag gagttcagac tcctgcaggc
     2821 agagctgaga gaatacggat tccgtactga aatcgccgga tatgatgccc tccggctgca
     2881 tacagagagc cggatgctct ttgctgatgc tgattctctt cgtatatctc cccgggaggc
     2941 caggtcgtta atcgaacagg ctgaaaaacg gcagaaggat gcgcagaaca cagacaagaa
     3001 ggccgctgat atgcttgctg aatacgagcg cagaaaaggt attctggaca cgcggttgtc
     3061 agagctggaa aaaaatggcg gggcagccct tgccgttctt gatgcacaac aggcccgtct
     3121 gctcgggcag cagacacgga atgacagggc catttcagag gcccggaata aactcagttc
     3181 agtgacggaa tcgcttaaca cggcccgtaa tgcattaacc agagctgaac aacagctgac
     3241 gcaacagaaa aacacacctg acggcaaaac gatagtttcc cctgaaaaat tcccggggcg
     3301 ttcatcaaca aatcattcta ttgttgtgag cggtgatccg agatttgccg gtacgataaa
     3361 aatcacaacc agcgcagtca tcgataaccg tgcaaacctg aattatcttc tgacccattc
     3421 cggtctggac tataaacgca atattctgaa tgaccggaat ccggtggtga cagaggatgt
     3481 ggaaggtgac aagaaaattt ataatgctga agttgctgaa tgggataagt tacggcaaag
     3541 attgcttgat gccagaaata aaatcacctc tgctgaatct gcggtaaatt cggcgagaaa
     3601 taacctcagt gccagaacaa atgagcaaaa gcatgcaaat gacgctctta atgccctgtt
     3661 gaaggaaaaa gagaatatcc gtaaccagct tgccggcatc aatcagaaga tagcggaaga
     3721 gaaaagaaaa caggatgaac tgaaggcaac gaaagacgca attaatttca caacagagtt
     3781 cctgaaatca gtttcagaaa aatatggtgc aaaagctgag cagttagcca gagagatggc
     3841 cgggcaggct aaagggaaga aaatacgtaa tgttgaagag gcattaaaaa cgtatgaaaa
     3901 gtaccgggct gacattaaca aaa
//
LOCUS       DADSZD010000264         3910 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.266, whole genome shotgun sequence.
ACCESSION   DADSZD010000264 DADSZD010000000
VERSION     DADSZD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.266"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            8..1666
                     /gene="dpiB"
                     /locus_tag="J1Z28_004305"
     CDS             8..1666
                     /gene="dpiB"
                     /locus_tag="J1Z28_004305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBA7719062.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKSVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1635..2315
                     /gene="dpiA"
                     /locus_tag="J1Z28_004306"
                     /gene_synonym="citB"
     CDS             1635..2315
                     /gene="dpiA"
                     /locus_tag="J1Z28_004306"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBA7719063.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2356..3741)
                     /gene="dcuC"
                     /locus_tag="J1Z28_004307"
     CDS             complement(2356..3741)
                     /gene="dcuC"
                     /locus_tag="J1Z28_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBA7719064.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1067 a          929 c         1005 g          909 t
ORIGIN      
        1 gaaaacaatg ttgcagctta acgagaataa acagtttgca tttttccaaa gactggcatt
       61 tccgctgcgt atctttttgc tgattctggt gttctcaata tttgtcattg cagccctggc
      121 gcaatatttt acggccagtt ttgaggacta tttaacgctt catgtgcgcg acatggcaat
      181 gaatcaggca aaaattattg cttctaatga cagtatcatc agcgcagtga aaacgcgtga
      241 ctacaagagg ctggcgacca tcgctgacaa attacaaaga gatactgatt tcgattatgt
      301 ggtgattggc gatcggcact cgattcgtct ttaccatcct aatccggaga aaattggtta
      361 tcctatgcag ttcaccaaac cgggcgcgct ggagaaaggg gagagctact tcatcaccgg
      421 gaaagggtca attggcatgg cgatgcgtgc caaaacgcca atctttgatg acgatggaaa
      481 agtcatcggc gtggtgtcga ttggctacct ggtgagtaaa atcgatagct ggcgtgctga
      541 gtttttatta ccgatggcag gtgtgtttgt cgtgctgtta gggattctga tgttgctatc
      601 gtggttcctg gccgcgcata tccgtcggca gatgatgggc atggagccaa agcaaatcgc
      661 acgcgtggtc cgtcagcaag aagcgctgtt tagttcggtt tatgaagggc tcattgcggt
      721 ggatccgcat ggttacatta ccgccatcaa tcgtaacgca agaaagatgc tgggtctgag
      781 ttcccccgga cggcaatggt tgggtaaacc cattgctgaa gtggtcaggc ccgccgattt
      841 ctttaccgaa cagattgatg aaaaacgtca ggatgtggtg gcgaacttta acggtctgag
      901 cgttattgcc aaccgggaag ctattcgttc aggtgatgat ttactggggg ccattattag
      961 ctttcgtagt aaagacgaaa tttccaccct caatgcgcaa ctgacgcaaa ttaaacaata
     1021 cgtcgagagc cttcgtacat tgcgacacga gcatctcaat tggatgtcga cgctcaatgg
     1081 tctgttgcag atgaaagagt atgatcgcgt gctggcgatg gtgcaggggg agtctcaggc
     1141 ccagcaacag cttattgaca gcctgcgtga ggcgtttgcc gatcgccagg tggcggggtt
     1201 gctttttggt aaagtgcagc gcgcccgcga actggggcta aaaatggtca tcgtccccgg
     1261 tagccagctt tcgcaactgc cgccaggact ggacagcacc gagtttgcag ccattgttgg
     1321 caatttactt gataacgcct tcgaagcaag cctgcgtagc gatgaaggaa acaagagcgt
     1381 tgaattattc ctcagcgatg aaggcgatga tgtggtgatt gaagtcgccg atcagggctg
     1441 cggcgttcca gagtctctac gagacaaaat atttgagcag ggtgtcagta cgcgtgctga
     1501 cgagcccggt gaacatggca ttgggttgta cttgattgcc agctacgtaa cgcgctgcgg
     1561 tggtgttatc actctcgaag ataatgatcc ctgcggtacc ttattttcaa tctatattcc
     1621 gaaagtgaaa cctaatgaca gctccattaa ccctattgat cgttgaggac gaaacgccgc
     1681 tggcagagat gcatgcggaa tatattcgtc acattcccgg attcagccag atattactgg
     1741 cgggaaacct ggcgcaggcc cgaatgatga tcgagcgttt taagccgggg ctaatcttgc
     1801 ttgataacta tcttcctgac ggtagaggga ttaatttact gcatgaactg gtgcaggcgc
     1861 attatcccgg cgacgtggtg tttaccactg cagccagcga tatggaaacg gtgtctgaag
     1921 ccgtacgttg tggtgtattt gattatctca ttaagcccat tgcctatgaa cggttggggc
     1981 aaacgctaac ccgtttccgc cagcgtaaac atatgctgga aagtattgat agcgccagcc
     2041 agaagcaaat tgatgagatg tttaatgcct atgctcgcgg tgaacccaaa gacgaactgc
     2101 cgaccggcat tgaccccttg acgctaaacg cggtgcgaaa actgttcaaa gagcctggtg
     2161 tgcaacatac ggcagaaacg gtggcgcagg cactgaccat cagccgcacc actgccaggc
     2221 gttatcttga atattgcgcc agccgccatc tgattattgc tgaaattgtt cacggcaaag
     2281 ttggcagacc gcaacgcata taccacagtg ggtgacatga taaagccgga ggaaacttgc
     2341 ctccggcatt actatttact tgcctgtgac cgctgctgcc gttcctggca ccatcagctc
     2401 tgtagcaacg ataacaatca ccaaaccaac aagcaccggc accgaggtgc gttttacgac
     2461 ttcaaacggc gagatcttcg ccatcccggc aaccgcaacg actacgccag aaacgggtga
     2521 tagggtacga cccaggtttg acgcctgcag catcgggata gtcaaatacg ccgggttaat
     2581 gccggaggag tgcgccagtt tcgggatcat ctcaacaaac gcataaaacg gcgcattgcc
     2641 tgaaccggtc gtgactgccg ccagcattgt caggatcacc aataccagca tcaggatgat
     2701 actcgccgaa ccaaacgagg tagcgataga aatcagactt tgaataaagc cgatggtgct
     2761 aagcccctga gcgaataccc cagcggcaac cagcagcatc accacgttag caaatgcatc
     2821 tgccataccg cgataagcca cttccagacc agagaaaact ttctgggtat taaagctgcg
     2881 gatgaactcc agaatggagg caattagcat acaaatcacc agaatagtga tgatgtgtaa
     2941 ttgcggaccc catttgccgt caaaaatcag cactccgatg atcggcgtga acggcaaaat
     3001 ggcataaaac gcaggggcag tggtggtgat ttcactgaca tctaacattt catgagagat
     3061 gtgctctttt ttatccagat aacgttgcca gaagaagtgg gcgattgcca tgccgataat
     3121 tgcagcaatt gagataggca gcgttgtttt gaaggcgaag tcaatcagcg acatttcgga
     3181 agcctgcgcc gccagcacca catcccctga agtcggtgcg agaataatcg ccgccgggga
     3241 ggcacaaatg gcagcagctg cgccacgact gataccaacg tttaccatca ccggaaacag
     3301 ggttgccatc agcaaaacac ccagaccggt tgcggaagag acagccagtg acatcagaca
     3361 ggcaacaaaa taggcggcaa tcatcagaag gtaaggggag ttaatatact gcaatggttt
     3421 tgacgccagc ttgaccacca tatcattcgc gccgatatgg gtcatgtaag cggcaaagcc
     3481 acacagcatc ataatcatca tgccgaggtc gccgccgcgg ctcattagca atattttaac
     3541 gtattcaacg atatccgtgg cgctgtagcc tgttgaagcc tggctggacg gtaacacttt
     3601 gtgccccata atggcactga taatcagcaa taacaggcca ccgacaaata acacgccagt
     3661 ggcagaatac cctttaatga tgtagcgagc tacacccaca ataaccacaa ccccaataag
     3721 gagttcaatg aatgtcagca taatttttcc tgtctccagg ccccaaagta aataataaaa
     3781 aattcctaaa agttaaggaa aaaatatgcc caataaattg gcggtgaatg ctgattaaaa
     3841 tcaagaaaaa ctgccattaa gacattgaag ttgctgtttt tatacgtaga taacaaaacg
     3901 catatttagc
//
LOCUS       DADSZD010000265         3881 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.267, whole genome shotgun sequence.
ACCESSION   DADSZD010000265 DADSZD010000000
VERSION     DADSZD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.267"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            4..387
                     /locus_tag="J1Z28_004308"
     CDS             4..387
                     /locus_tag="J1Z28_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719065.1"
                     /translation="MYNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQ
                     VLDEIQTPSAINSELYTKMIYLTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFF
                     LSIVISVMFCLVKKMVDEYQQNSGQ"
     gene            complement(630..1325)
                     /locus_tag="J1Z28_004309"
     CDS             complement(630..1325)
                     /locus_tag="J1Z28_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBA7719066.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1318..2745)
                     /locus_tag="J1Z28_004310"
     CDS             complement(1318..2745)
                     /locus_tag="J1Z28_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBA7719067.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQVQENKNG"
     gene            complement(2756..3475)
                     /locus_tag="J1Z28_004311"
     CDS             complement(2756..3475)
                     /locus_tag="J1Z28_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBA7719068.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLMLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1014 a         1025 c          814 g         1028 t
ORIGIN      
        1 tttctgtata acagagaaat aaagctggaa tggtcaggag ataagagaga tttaccaact
       61 gctgaagcgg aaatatctcg cgtgcaggcg tctatcatca aatggtatgc gtcagaatac
      121 cataatggca ggcaagttct cgatgagata caaacgcctt cagcaattaa cagtgagctt
      181 tatacaaaaa tgatatacct gaccaggaac tggtcactgt atccgaacgg tgatggctgt
      241 gtaaccataa gctcaccgga aataaaaaat aaataccctg ctgccatttg cctggctctg
      301 ggattttttc taagtattgt gatttctgta atgttttgcc ttgtcaaaaa aatggtagat
      361 gaataccaac aaaactctgg gcagtaataa acagcattct gacagagagt gcctgtataa
      421 aactatagtg acagtgaccg ggaagggagc ccgtcttcag gaatggattc ctgtcttttt
      481 aactgtaaat atcctggcat tattaatgtc aggatattta cagaagagca gcataaccat
      541 gacggaacgg ggttaagtca ggttgtcctt aacgttattc acaacctcgt ataccgcctt
      601 caggtttatg tccagactac atatctctct cagcaatcct caataatcag atacaccgct
      661 ttcaccggac catgaacccc gacgactttg ataagctcaa tgtccgccgt tgaactggga
      721 ccactgatga tgttaataca tgacggcatt cgttcaccgg cctgcgcttt ctgatgcaat
      781 ttttctgcga gttgcgctac acgcggcagg attgtgcttt tacgcaggat aaaaagagaa
      841 gattccggga gcaggctcaa tgaacgcccg cgcccggcgg cggaaaaaag aaccacgcct
      901 cccgattcgg ttaaaccata ttcagcatat acgacgccca ctttagcctg ctctgcttgt
      961 gatatgttct caataccttt cgccggatcc caaacaacgg cattgcattc ctgctgcaaa
     1021 cgttcgctaa tccccaattc ctccagcctc gtgtcaccac taatcacgac cgactgatct
     1081 cccagctctt tacacagacg tattgcagct tctgccgcct tcgcctcgct ggtcagttca
     1141 cagcgcgtca acataacatc gctggcaaac tggataaacg cgtcacagcg ctgctgttgg
     1201 gtaagttggg taagccgctc gttagcatag ttgttaagcg gcgcatcttc tgcttgcggt
     1261 tcaagtcgca gcgggcgacc cagtgcctga gcaacgttat tcaaaaattc gctccgatta
     1321 tccattctta ttctcctgca cctgatgttt cttaaaccaa ctacggaaac tctctccgtc
     1381 agcttcagga agatcgcgtg cttccatcca gtcgctaatc gcgccaaatt tgagtggtgt
     1441 tttgccgcca ttgataaacc agcttgccgc atgagcacca gccatcatcc cgactttcca
     1501 caatcctgga tgactattgg cataagcgaa catttttatc gcccgttgct ctgcttttgc
     1561 ggtgatccct ttttcagcca tcacccgacg atgacgcaaa atcagttttg acagcggaat
     1621 acgcaccgga cacacgctgt cacaagcggt gcataaagag caggcgtagg gtaaatcttt
     1681 aaaatcttta tagccgccaa gtaacgggga aatcaccgca ccaattggcc ctggataaat
     1741 agagccatat ccatgaccgc caatatggcg atatgccgga caagtattca tacaagcccc
     1801 gcaacgaata cagcgcagca catcccgaaa ttcagaggcc agcacctcag aacgcccgtt
     1861 atcgacaata accagatgaa actcttcagg accatcaacg tgccccgctt cgcgcggtcc
     1921 tgtcagccag gtgttgtatc ccgtcaaacg tgcaccaacg gcactgcgcg ccagcatggt
     1981 gatcaataca tctacctctg caaacgtggg agcaatacgc tccattccca tcactgcaat
     2041 atgcgtttta ggcagcgtgg tacacattcg cgcattacct tcattggtca ccaggcatac
     2101 cgaaccggtc tctgccaccg cgaaattaca gccggtaata cctatttcag cactgaggaa
     2161 atcttcgcgg attttttgcc gaatgaataa ggtcatcgct tcaggcgttt ccggcccctc
     2221 atagcccaga cgttcgtgta gcactcgacg gatctgatgg cgatctttat gaattgccgg
     2281 gaccacaaca tgagagggcg gatcttgatc cagctggaga atgtattcac ccagatcggt
     2341 ttcaatcacc tgaatcccgg catcctgcaa cacatgattg acaccaatct cttcggtcac
     2401 catcgattta gatttcacta ccttccgggc atttttgcgt tgggcaacct gtaaaatgta
     2461 gcgggtggcg tcttctttgg tttttgcaaa atagacgtga ccgccgtttt gtgtcacttt
     2521 ttctgagagc tggtacagat aagcgtcgag attactcaga acatgatcac gtatctgggc
     2581 ggcccgatcg cgccactcct cccagtgccc caattcatcg accatttttt gccgatttgc
     2641 cccaatacgc tgctgcgcgt ttgccaccgc tttacgcatg atcggatctt caatttgctg
     2701 acggatgcgt gtcttaaaat ctgtattact ggttttgatc gacatcttta tatcctcagc
     2761 ggctcatcag cacttcagca atatgcatca ctttgacttt ctgcccttcc cgttgtaatc
     2821 gtccactgat gttcagcagg caactcacgt cagcaccaat taaatactca gggcggactt
     2881 ccatcaggtg cgcaaccttt tctttcacca tctcgccgga tatttcggcc attttgaccg
     2941 agaacgtgcc gccaaatccg cagcaggtat cctgttcagc aaaggtcaac agctccagtc
     3001 cacgcacatt tttcagcagc ataagtggct cgtccttcac ccccagctta cgggccaggc
     3061 tacaagatgg gtgatacacc gctctccctt gcaaactggc acctacatcg actaccccta
     3121 atgtattaac aataaaagag gtgagatcct gcatacgcgc ggcaaccttt tcggcacgtg
     3181 atgcccattc aggctcatcc gccagatacg ttgggtaact ttttacggca taggtacagg
     3241 agccagccgg tgaaataatg ggatcgtcgt tatcctccag tgcggcgatc agatttttca
     3301 tccctggaat cgcttctttg atataaccac tattgatcgc aggctgaccg cagcatccct
     3361 gtttctccgg gaaatttacg cgacagccga gtttttccag tagcagcacg gagtctcgtg
     3421 ccattcttga tttcagggcg tcaccaatac aggtgacaaa gaaattgaca ttcacaacta
     3481 atactccatt acttcatgcc catttatgcc cgatggttcc ccccaccggg ggatcaatca
     3541 tcaataaata tggcgttctt ttttaattgg ctagcaacat accacccctc atttgtatga
     3601 caaatgataa tctttcatcc aaacttcaaa tgcaataact aattttattt ctttcggcat
     3661 cgctcacatt ttatggggaa atcgacgatc atttttggca taagaaatcc atttaaaaat
     3721 ggctacatcc cttataccta ttcagagcta cttattcagg ggttattgag atatacactg
     3781 atggtgtaca aaaaaatcac cgacaataat cacgtatcaa gtaatagtcg gcattttatt
     3841 taattgaagc aatttagttt gcatttttat ttaaaaactg a
//
LOCUS       DADSZD010000266         3622 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.268, whole genome shotgun sequence.
ACCESSION   DADSZD010000266 DADSZD010000000
VERSION     DADSZD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.268"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..445
                     /gene="nanC"
                     /locus_tag="J1Z28_004312"
     CDS             <1..445
                     /gene="nanC"
                     /locus_tag="J1Z28_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBA7719069.1"
                     /translation="WTVRPGMLTHFSSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKA
                     YRQQDLSGDMSRDNVHRWDGYVTYHINSDFTFAWQTTLYSKQNDYRYANHKKWATENA
                     FVLQYHITPDITPYIEYDYLDRQGVYNGRDNLSENSYRIGVSFKL"
     gene            465..1571
                     /locus_tag="J1Z28_004313"
     CDS             465..1571
                     /locus_tag="J1Z28_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBA7719070.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNQ
                     AGKDSTAIDKINTHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1636..2616
                     /locus_tag="J1Z28_004314"
     CDS             1636..2616
                     /locus_tag="J1Z28_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBA7719071.1"
                     /translation="MNAIISPNYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHLGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPYNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQFHSQLNNIT
                     AAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYFGSAYRSAENWTTSLRSSHFSAAARRGIISDRFVEAILQFWRER"
     gene            complement(3199..>3622)
                     /locus_tag="J1Z28_004315"
     CDS             complement(3199..>3622)
                     /locus_tag="J1Z28_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HBA7719072.1"
                     /translation="GAQKKITIISPWLSWQKVEQTGFLASMALARSRGIDITVVTDKN
                     CNIAHVDDDKRQEKQHLLNDAVEKLNKMGIATKLVNRVHSKIVIEDEELLCVGSFNWF
                     SAAREDKYQRYDTSLVYRGEGVKNEIKAIYGSLDQRQL"
BASE COUNT         1046 a          819 c          816 g          941 t
ORIGIN      
        1 atggacggtg cgcccgggaa tgttaacgca ttttagcagc aacggcacac gctacggacc
       61 ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat tttggcattc gctatcgtta
      121 cgactggaaa gcttaccgac aacaagactt atccggtgat atgtctcgtg ataacgttca
      181 tcgttgggat ggatatgtca cttaccatat taatagtgat ttcaccttcg catggcaaac
      241 gacgctatac agcaaacaga acgattatcg ctatgcaaac cataagaaat gggcgacgga
      301 aaatgcattt gttctacaat accatataac gcccgatatt acgccataca tagaatatga
      361 ctacctggac cgtcagggtg tttacaacgg cagagataat ttatcggaaa acagttatcg
      421 cattggtgtg tcatttaaac tgtagtagac aggagacagt cacaatgaat aaaacaataa
      481 cggcgcttgc gatcatgatg gcttcatttg ccgcaaacgc gtctgtatta ccggaaactc
      541 ctgtgccatt taaaagtggt accggagcaa ttgataacga cactgtctac attggtttag
      601 gtagcgcagg tacggcatgg tacaagctgg atacacaggc caaagataaa aaatggacag
      661 cgttagctgc attccctggt ggaccaagag atcaagcaac ctcggcattt attgatggca
      721 atctttatgt gtttggcggc attggcaaaa acagcgaggg cttgactcag gtatttaatg
      781 acgtacacaa atacaacccc aaaaccaata gctgggttaa attgatgtcg cacgccccga
      841 tgggcatggc gggccatgtg acttttgtac acaacggcaa ggcttatgtt actggcggtg
      901 ttaaccagaa tatcttcaat ggctattttg aagatctcaa ccaggctgga aaagattcaa
      961 ccgctataga taaaatcaac acccactatt ttgacaaaaa agcagaagat tatttcttca
     1021 ataaatttct gttgtctttt gatccctcaa cacagcaatg gagttacgct ggcgaatctc
     1081 cctggtacgg aacggctggt gcggcggttg tgaataaagg tgataaaacc tggcttatta
     1141 atggcgaagc caaacccgga ttgcgaacgg atgccgtatt tgaacttgac ttcaccggta
     1201 ataatttaaa atggaataag ctagctcccg tctcatcacc agatggcgtc gctggcggct
     1261 ttgcaggcat gagcaatgac tcactgatat ttgccggagg ggccggattc aaaggttcac
     1321 gagaaaatta ccagaacggt aagaactatg cgcatgaagg cctgaaaaaa tcatatagca
     1381 ctgatattca tctttggcat aacgggaaat gggataaatc gggtgagtta tcgcaaggtc
     1441 gggcctacgg agtatcattg ccctggaata atagtctatt gattattggc ggtgaaactg
     1501 caggcggcaa agcggtgacg gattcagttt tgatctctgt gaaggataat aaagtcacag
     1561 tacaaaacta acgcttcagg gccccggtaa aggggccctg ctatcaattt gtcatttcaa
     1621 ttacgagaaa attatatgaa cgcaataata tcgcccaatt attactatgt tcttaccgtt
     1681 gctggtcagt ctaatgccat ggcgtatggc gaaggactgc cattaccgga cagggaagat
     1741 gcgcctcatc ccagaatcaa acaattagcg agatttgcgc atacgcatct cggaggcccg
     1801 ccatgtcact ttaacgacat tattccactg actcactgcc cacacgatgt tcaggatatg
     1861 cagggttatc accatcctct ggcaacgaat catcaaacac agtacggcac cgttggccag
     1921 gcactgcata ttgcacggaa attactgccc ttcattcctg ataatgcagg gattctcatc
     1981 gttccgtgtt gccgtggcgg atcggctttt accgcgggca gcgaggggac atattcagaa
     2041 cggcacggag ccagccatga tgcttgtcgt tggggaacgg ataccccgct ctaccaggat
     2101 ttagtcagca gaacgcgagc cgcactggca aaaaatccgt ataacaaatt cctcggcgta
     2161 tgctggatgc aaggcgaatt tgacttaatg accagtgact acgcgtcaca ccctcaacac
     2221 tttaatcata tggttgaagc ctttcgtagg gatctaaaac aattccattc tcagcttaat
     2281 aatattactg ccgcgccgtg gttttgcggc gataccacct ggtactggaa agaaaatttc
     2341 cctcatgcgt atgaagctat ttatggcaat tatcaaaata atgttttagc caatattatt
     2401 ttcgtcgact tccagcaaca aggtgaaaga ggactgacga acgcgcctga tgaagatccg
     2461 gacgatttaa gcacgggata tttcggttca gcgtaccggt cagcggagaa ctggacgacg
     2521 tcactgcgaa gcagtcattt cagcgcggca gcccgtcggg ggattatttc tgacagattt
     2581 gtagaagcaa ttttgcagtt ttggcgcgaa aggtgagcgg ggagtttatt catgggggat
     2641 gtccttgtcc gttcccagag cagacaacaa aaaacaaatg agatcaaggt aatatctctt
     2701 cacttgcggg ctcgaacagc aggcaattgc caatataata atctggcaca agggtgaaaa
     2761 acgcgcaccg cataaaccgt tgttatttct tacagattag ccggatgcct taatagacat
     2821 ccgcgccttt tcggttatgg ctcggaaatc tacgaacatc tacacagttt actggaacgt
     2881 tttgggccac agcgttcgca gcaccgtcct gatatgccgt tctggcgatt ataagtcgac
     2941 ggattctggc aaatagacga tgatgatatg cttaatgatg ttcaaagaaa ttaggccatt
     3001 attatattat tgcttgcatt ttctttcatt cctaaaagca cccgccatta atcccaggcg
     3061 cattatgcaa aggaaaatga ttaggtaaac aataagattt ataatcagga aaggcgactc
     3121 acaataaaac ggaaagtcgc cttaacattg gtaaaatatg ccctttacta ctcctgtatc
     3181 atgaacggct catcacattt aaagctgacg ttgatccaga ctaccatata ttgcctttat
     3241 ttcatttttt acgccttcgc cacggtacac caaagacgta tcgtatcgtt gatacttatc
     3301 ttcccgtgca gcactgaacc agttaaaaga tccgacacac agcagttctt catcctcaat
     3361 cacaattttg ctgtggacac gattaaccaa tttcgtagcg atacccatct tattgagctt
     3421 ttcaacagca tcattcagca gatgttgttt ctcctgacgc ttatcatcat cgacatgcgc
     3481 tatattgcag tttttatccg tcaccacggt aatatcaatg ccccgggaac gggccaaagc
     3541 cattgatgcc agaaatcccg tttgctccac tttttgccaa gataaccaag gggaaataat
     3601 cgttattttc ttttgagctc cg
//
LOCUS       DADSZD010000267         3593 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.269, whole genome shotgun sequence.
ACCESSION   DADSZD010000267 DADSZD010000000
VERSION     DADSZD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.269"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            526..1491
                     /gene="lipA"
                     /locus_tag="J1Z28_004316"
     CDS             526..1491
                     /gene="lipA"
                     /locus_tag="J1Z28_004316"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBA7719073.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(1591..1794)
                     /gene="tatE"
                     /locus_tag="J1Z28_004317"
     CDS             complement(1591..1794)
                     /gene="tatE"
                     /locus_tag="J1Z28_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBA7719074.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(1923..2711)
                     /locus_tag="J1Z28_004318"
     CDS             complement(1923..2711)
                     /locus_tag="J1Z28_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBA7719075.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMVQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            2804..3187
                     /gene="crcB"
                     /locus_tag="J1Z28_004319"
     CDS             2804..3187
                     /gene="crcB"
                     /locus_tag="J1Z28_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBA7719076.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(3241..3450)
                     /gene="cspE"
                     /locus_tag="J1Z28_004320"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(3241..3450)
                     /gene="cspE"
                     /locus_tag="J1Z28_004320"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBA7719077.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
BASE COUNT          935 a          923 c          824 g          911 t
ORIGIN      
        1 agaaaatcgt cagaaggtaa accacaaata tttacaacct tacgaaatat tgatcttact
       61 cttctgacta ttttgaagtt gcatatgtcc ataaaggaat cgttaatgca gcgaaagtgc
      121 ttaatctgcc ccccctcggt cggagccctg gtccttcctg tcatctatcg ggcgattaaa
      181 actcactatc cgcagctttt actgcgcaac ccgcccatca gcggcgcgga aaaccaactc
      241 agtcagtttc aaaccgatct catcaccgat aacatgtttt gcacaggacc atagatgtta
      301 tctgtaatgc attttgaaaa agtattgtta cagccagatc acagcgcaga acgataaaat
      361 ggcacaatcc aacaacaatt ttacattttc gcgaccgctt tggctgcttt caggtccgtt
      421 tcaatgatat actgccagtc gttaattcaa aaatagttga taattacaac aatctattga
      481 attgaaacgc tttccttcgt aattcgcaac tggaacacgc acgctatgag taaacccatt
      541 gtgatggaac gcggtgttaa ataccgcgat gccgataaga tggcccttat cccggttaaa
      601 aacgtggcaa cagagcgcga agccctgctg cgcaagccgg aatggatgaa aatcaagctt
      661 ccagcggact ctacacgtat ccagggcatc aaagccgcaa tgcgcaaaaa tggcctgcat
      721 tctgtctgcg aggaagcctc ctgccctaac ctggcggaat gctttaacca cggcacagca
      781 acgtttatga tcctcggcgc tatttgtacc cgccgttgcc cgttctgtga cgttgcccac
      841 ggtcgcccgg tagctcctga tgccaatgaa ccagtgaaac tggcgcagac cattgccgat
      901 atggcgctgc gttatgtggt tatcacctcc gttgaccgtg atgacctacg cgatggcggt
      961 gcccagcact ttgcggattg cattactgcc attcgggaaa aaagcccgca aatcaaaatt
     1021 gaaactctgg tgccggattt ccgcggtcgt atggatcgtg ctctggatat tctgactgca
     1081 acgccaccag atgtgttcaa ccataacctg gaaaacgtac cgcgtattta ccgtcaggta
     1141 cggcctggcg cagattacaa ctggtcgctt aagctgctgg aacgctttaa agaagcgcat
     1201 ccggaaatcc cgaccaagtc tggtctgatg gtgggactgg gtgaaaccaa tgaagaaatt
     1261 attgaggtaa tgcgcgacct gcgccgtcat ggtgtgacga tgctaacgct ggggcaatat
     1321 ttgcagccaa gccgccatca cctgccggtt caacgttacg ttagcccgga tgagttcgac
     1381 gaaatgaaag ccgaagcgct ggcgatgggc tttacccatg ctgcatgcgg tccgtttgtc
     1441 cgctcttctt accacgccga tttgcaggcg aaagggatgg aagttaagta atcctgtaat
     1501 aattacttac atctgtctga taaaaaaccc gctttttgaa gcgggttttt gtatcgaaca
     1561 agatattgag ggagcgtcct gctcgccacg tcactcttta tgagagagct tttcagcctg
     1621 aagatcaacg tctgcgcctt ttttcgccgc agcatcgtca tcattcatcg ccttcttgaa
     1681 ccctttaatg gccgctccaa ggtctccgcc cagcgtacgt aacttcttag tcccaaacag
     1741 cagaacgacc agcgccgcaa ctaccagcag tttggtaata ctaatctcac ccatagatac
     1801 cttcttgaca tataaccggc aacagaacgc gccttaaaaa ccagagaata ttaacggtca
     1861 ttcgacgcgc gcacacaata gcaatctgta acaaggtgaa tcaagcgccg agtaaaaaaa
     1921 catcataata attgcggcgg cgcaaagcga cggttgttta agacgggcag ttgcgcgcgt
     1981 acctgacgca cacgttcggg cgtcacttcc gccataatga gtgcaggcat ttctgacgct
     2041 gccgcaatgg tgacgccaaa gggatctata atccggcttt gcccgatatt tttgttcccg
     2101 cactcccccg ccgccaccat ataacaggtg gtatccagcg cacgagcggc aagcaacgtt
     2161 gaccagtgat gctctttgag cggcccacga acccaggcgg caggaagtac caggatttca
     2221 gctccctgta atgcctgtgc cagcgccagc tctggaaagc gtaagtcata acaggtcatc
     2281 agaccgacct tcatcccttc cacctccagt aacggagcta tttcattacc ggcatcaaca
     2341 tggcgtgatt cctgaatggc aaatgcatca tagagatgca gtttggcata acgggcgacg
     2401 atgtttcctg cctgtagcgc caccagcata ttccatgcgc gccccggcgt tgaaggaaca
     2461 tgaatcgtca gaattgtcgt catcatgtta cgtttacttt ctcgccgtaa acggtcgagg
     2521 aattcgcctt ccagcagctg tgctgattta accgaaagat ccgcatcatg atcatcacgc
     2581 gccagtaatg cttccggcag gacaaacagc gaaacctcgt tttccgccgc ctgcaccatc
     2641 aacgaggcac aaatctcagc gttcttttcc cacacagatg taacagcaaa ctgtcctgcg
     2701 gcaaccagca ttgccacctc ctgtcagagt cgtaaaattc gccaacggcg attattcggc
     2761 gttacactta tcactcatac aaatcaaata gcaggatttt gcagtgttac aacttctttt
     2821 agcagttttt attggcggtg gtacgggaag cgtggcgaga tggctattaa gtatgcgatt
     2881 taacccgctg catcaggcga ttccgttggg gacgctggca gcaaacctga ttggggcatt
     2941 catcatagga atgggattcg cctggttcag caggatgacg aacattgatc cagtgtggaa
     3001 agtattaatc accaccggat tttgtggcgg tctaacaacc ttctcaacat tttcggcaga
     3061 agtggtgttt ttgttacaag agggccgctt tggctgggcg ttactgaacg ttttcgtcaa
     3121 tcttttgggg tcttttgcta tgaccgcact ggctttctgg ctgttttcgg cctcaaccgc
     3181 acactaaaaa ccaaaaaaaa cccgctgatt aagcgggttt tgaattcttg ctgacgtatc
     3241 ttacagagcg attacgtttg cagcagaagg gcctttggca ccgttagtga tttcgaactc
     3301 tacgcgctga ccttcagcaa gagttttaaa accattagtc tggattgcag agaagtgtac
     3361 gaacacgtct ttgctgccgt cttccggagt aatgaaaccg aatcctttgg actcattaaa
     3421 ccacttaacg ttacctttaa tcttagacat caaaattacc tttacatgaa aaatagacac
     3481 aaatgctgtg tcggttacca gtacaccaat tgtggtacgc tttgtccagt gcaactttac
     3541 taaaaagtga taaaagtcgc gttatttttg atgttaatta ttttactggt tga
//
LOCUS       DADSZD010000268         3573 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.270, whole genome shotgun sequence.
ACCESSION   DADSZD010000268 DADSZD010000000
VERSION     DADSZD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.270"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            69..1307
                     /gene="alaC"
                     /locus_tag="J1Z28_004321"
     CDS             69..1307
                     /gene="alaC"
                     /locus_tag="J1Z28_004321"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBA7719078.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            complement(1372..1443)
                     /gene="ypdK"
                     /locus_tag="J1Z28_004322"
     CDS             complement(1372..1443)
                     /gene="ypdK"
                     /locus_tag="J1Z28_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBA7719079.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(1799..2719)
                     /gene="lpxP"
                     /locus_tag="J1Z28_004323"
     CDS             complement(1799..2719)
                     /gene="lpxP"
                     /locus_tag="J1Z28_004323"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBA7719080.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPADENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3072..3314
                     /locus_tag="J1Z28_004324"
     CDS             3072..3314
                     /locus_tag="J1Z28_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBA7719081.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            complement(3391..>3573)
                     /locus_tag="J1Z28_004325"
     CDS             complement(3391..>3573)
                     /locus_tag="J1Z28_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719082.1"
                     /translation="EASYKETEKTSVMTTTHSTKLQPSQAILFKMIEDAPPLNLTEDI
                     TSTYPTKANYLIHPLR"
BASE COUNT          876 a          851 c          924 g          922 t
ORIGIN      
        1 tcaaaacagc caccatcgtg gcagcgtcgc tcggacggtc cgggcgctaa cgttaatctg
       61 aggaaattat ggctgacact cgccctgaac gtcgctttac gcgcattgat cgtctcccgc
      121 cctacgtttt taacattacc gctgaactga aaatggctgc gcgtcggcgc ggcgaagata
      181 ttatcgattt cagtatgggt aacccggacg gtgcgactcc gccgcatatc gtcgaaaaat
      241 tatgtacggt ggcccagcgc ccggacacgc atggttactc cacttcacgc ggcattccgc
      301 ggttacgtcg cgccatttcc cgctggtatc aggatcgcta cgacgttgaa atcgacccgg
      361 aatcagaagc catcgtcact attggttcga aagagggcct ggcgcatctg atgctggcga
      421 cgctggatca tggtgacaca gtgctggtgc cgaatccaag ctaccccatt catatttacg
      481 gcgcggtgat tgctggggcg caggtacgct cagtgccgct ggtggaaggt gtcgacttct
      541 tcaatgaact ggagcgcgcc attcgtgaaa gttatccgaa accgaagatg atgatcctcg
      601 gcttcccgtc aaacccaacc gcgcaatgcg tggaactgga attctttgag aaggttgtgg
      661 cgctggctaa acgctacgat gtgctggtgg tccacgacct ggcctatgcc gatatcgtct
      721 acgatggctg gaaagcgccg tcaatcatgc aggtgccagg tgcacgcgac gtggcggtcg
      781 agttctttac gctgtcgaaa agctacaaca tggcgggctg gcgtatcggc tttatggtcg
      841 gtaacaaaac gctggtcagc gcgttggcac gtattaaaag ctatcacgat tacggcacct
      901 ttacgccgtt gcaggtggcg gctattgcgg cgctggaggg cgatcaacag tgcgtgcgcg
      961 atattgccga acagtacaaa cgccgccgtg atgtgctggt gaaagggctg catgaagcgg
     1021 gctggatggt cgaaatgccg aaggcttcga tgtatgtctg ggcgaaaatc ccggaaccgt
     1081 atgctgccat gggctcgctg gaatttgcca agaaattgct taacgaagcg aaggtctgtg
     1141 tctctccagg gattggcttt ggcgactacg gcgatacgca tgtgcgcttt gcactgattg
     1201 aaaaccgcga ccgtattcgt caggcgattc gtgggattaa agcgatgttc cgtgccgacg
     1261 gtttactacc tgccagcagc aaacatattc acgaaaacgc ggaataaaca ccatcgtcag
     1321 aaatgcaaac aggagccaga tggctcctgt ttttcattgc acagtttgct tttagatcat
     1381 cagggcaaaa gtaccggccc aaacgatgac cataaaagaa atgcccataa agaaatattt
     1441 cactttacat ctctccgcgt aattactgtt acgcctgaat gacttcatta cacctgatac
     1501 agtgagttga caccgggcaa aacgacctta aacgtcctgt attttaccgt gggcatcacc
     1561 ccagaattct gagctagact gctcgagacg gcgctaatgt acgcctaaaa ctgaaacgag
     1621 acaagaggca ttttcttatt tacttttagt aacttacagg tgtctcatat cggcgacagt
     1681 ttaatttctg acggtaataa ttagatattc agcgaaggaa aaaaccttaa gaaaaaagcc
     1741 ataaaaacca tgaggttatt atggctgatt tgaggaggga aagagtgagc gccgtttgtt
     1801 aaatgtataa cgacgattca cccaccgggc gcgttttaaa gcgacggtgg atccagaggt
     1861 actgctccgg tgcgcgcatg atctctttct cgataatctt gttcatatag gcagcggctt
     1921 gattttcatc tgccgggtag ccttccatct ctggggtgat gtacaaacga tatccgctgt
     1981 aatccgcttt tcttaccatc gttacggtca acatggctgc gccagagaga cgagagagga
     2041 cataggtgcc attggttgtg gcgacatttt ccaccgcaaa gaacggcgcg aaggagctgc
     2101 ctttacgacc ataatcctga tcgggagcaa accataccgc ttcacctttc ttcagtgcac
     2161 tgacaatgcc gcgcagatta tttctgccga tcatcgcttt gttagagcgc atgcgaccac
     2221 gggtctgcac ccattccatc agctgattat tatgcgggcg ataggtggcc atcattggtt
     2281 ggcaaagtcc catcacccga ccgcccagtt ccagcgacat aaaatggacg ccgacaacca
     2341 ttacgccgcg attttgcatt tgtgcgcgtt taaggttatc cagcccttcc acatcaaacc
     2401 atttacgtac gcgactgtcg ggccagaacc acgccatgcc ggtttctacc agcgccatgc
     2461 cgagtgaacg aaagttttcg gcaatcatct tctcgcgctc tttcgcagaa tgctgcggga
     2521 aacaaagttc caggttttta cgggcgatag attcacgacg tttcaggaat ggtcgcgcca
     2581 ttgtgccgat acgcgtgccg agaaagcaga gaacaggata aggaagctgt acccaaagcc
     2641 agagtacacc aagcccaaac cacgtgagcc agtagcgagg atgcagaaac tcgcgggaaa
     2701 atttgcattt tggaaacata gttaccttct gtagatacga attgttgtat cgcagaagat
     2761 tccatgtcga cagcggtaca ctcaattgat taggccatca tgtacgacaa tggttcctgt
     2821 aaagagtgta aagactagtc aggacataac cacgaggagc atgaatgcgg tatctgtact
     2881 ttatcactcg ctgactgctt tcagctactg attattaatc gtgcaaagta aatatttttc
     2941 cgtacgtaac agtcttgcat ttaacatgct gttttgattg atgcttagca aaaattgcca
     3001 gaggttaagg attgtcttac tattaaagtg actgaagtta caggtttttt agcgcggcag
     3061 gaggttgagc tatgatttat ttatggatgt ttctcgccct gtgtattgtc tgcgtaagcg
     3121 gatacatcgg tcaggtactg aacatggtat cggcgatctc ttctttcttc ggcatggtga
     3181 tcctcgctgc actcatttat tacttcacca tgtggttaac tggcggtaat gaactggtga
     3241 cggggatatt tatgtttctt gccccggctt gtggcttgat gattcgcttt atggtggggt
     3301 atggcaggcg atagtgggtc aggatggctc atttgggcag gtgttcacat cagcatcaga
     3361 catgattgtt cacctgccag gtgacagacg ctatcgcaaa ggatgaatga gataattcgc
     3421 ctttgtcgga taagtagacg tgatgtcttc tgtgaggttt aatggtggtg catcttctat
     3481 catcttaaac aaaatcgcct gtgatggctg aagttttgta gaatgcgttg tcgtcatcac
     3541 tgacgttttt tctgtctctt tatagctcgc ctc
//
LOCUS       DADSZD010000269         3547 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.271, whole genome shotgun sequence.
ACCESSION   DADSZD010000269 DADSZD010000000
VERSION     DADSZD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.271"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..2375
                     /locus_tag="J1Z28_004326"
     CDS             <1..2375
                     /locus_tag="J1Z28_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBA7719083.1"
                     /translation="SRFSRAGYIMPGQYQMEIRVNDQNISPSAFQITFLEPPYSDSDN
                     EKPLPQPCLTPEIVSRMGLTEVSQEKITYWNNGQCADLSQLPGVEIRPNPAEGMLYIN
                     MPQAWLEYSDASWLPPSRWDNGIPGLLFDYNINGTVNKPHKGKQSQSLNYNGTAGANF
                     GAWRLRADYQGNLNHSTGSAQGTDSQFTWSRFYMYRAIPRWRANLTLGENYINSEIFS
                     SWRYTGASLESDDRMLPPRLRGYAPQVSGIADTNARVVISQQGRILYDATVPAGPFTI
                     QDLDSSVRGRLDVEVIEQDGRKKTFQVDTAYVPYLTRPGQVRYRLVSGRSRNYEHTTE
                     GPVFATGEASWGISNKWSLYGGGIVAGDYNALAVGLGRDLNEFGTVSADVTQSVARIP
                     GEETKQGKSWRLSYSKRFDDVNTDITFAGYRFSERNYMTMDQYLNARYRNDFTGREKE
                     LYTVTLNKNFEDWKTSVNLQYSHQTYWDRRTSDYYTLSVNRYFDAFSFKNIALGISAS
                     RSKYQNRDNDSAFVRLSVPWGTGTASYSGSMSNDRYTNTVGYSDTLNKGLSSYSLNAG
                     VSSGGGQPSQSQMSAYYNHSSPLASLSANFSAVENSYTSFGMSASGGATITAKGAALH
                     AGGMNGGTRLLVDTDGVGGVPVDGGRVSTNRWGIGVVTDVSSYYRNTTSVDLNRLPED
                     MEATRSVVESVLTEGAIGYREFEVLKGSRLFAVLRMADNSHPPFGASVTNAKGRELGM
                     VADSGLAWLSGVNPGETLNVGWDGRTQCVVDIPAHPDPAQQLLLPCRQVK"
     gene            2444..3175
                     /locus_tag="J1Z28_004327"
     CDS             2444..3175
                     /locus_tag="J1Z28_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012849081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7719084.1"
                     /translation="MQKMKPALKKTLMAVACLSAVPAAQAAISLDRTRAIFNGDEKSM
                     TLNIANDNKQLPYLAQAWVENEKKEKITTGPIIATPPVQRLEPGSKSMVRLTSTPDIS
                     RLPQDRESLFYFNLREIPPKSDKANVLQIALQTKIKLFYRPESIKAKPNAVWQDQLVL
                     NKTGGGYQIDNPTPYYVTVIGIGGGEKQAREGEFDAVMLAPKSTQMVKSGTYSTPYLS
                     YINDYGGRPVLQFSCAGSRCTAVKK"
     gene            3212..>3547
                     /locus_tag="J1Z28_004328"
     CDS             3212..>3547
                     /locus_tag="J1Z28_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease PIN"
                     /protein_id="HBA7719085.1"
                     /translation="MNRATIGFYLAVVLGSCSMNGVSQADELLTRDDFFVADESRHQW
                     VNEHNGRTGALNVKGALVSSPCQLDTNEVQISERKKRRDIQKVKINMSGCGYGNVPES
                     FSEWNRKLYV"
BASE COUNT          889 a          876 c         1039 g          743 t
ORIGIN      
        1 tttcccggtt ctcccgggcc ggctatatta tgcccggtca gtaccagatg gaaataaggg
       61 taaatgatca gaacatttct ccgtcggcat ttcagataac gtttcttgaa ccaccttatt
      121 cagattcgga taacgagaaa ccgttacctc agccctgcct gacaccggag atagtcagcc
      181 ggatgggact gactgaggtg tcacaggaaa aaattacata ctggaataac gggcagtgtg
      241 cggacctcag ccagttgccg ggggtggaaa tacgcccgaa tccggcagag ggaatgctgt
      301 acattaatat gccgcaggcc tggctggaat attccgacgc ctcctggctg ccgccgtccc
      361 gctgggataa cgggattccg gggctgttgt ttgactacaa catcaacggg acagtgaaca
      421 agccgcacaa gggaaagcag tcccagtcgc tgaactataa cggaactgcg ggggctaact
      481 tcggtgcctg gcgtctgcgt gcggactatc agggcaacct gaatcactcc accggcagtg
      541 cgcagggaac ggacagccag ttcacctgga gccgtttcta catgtaccgc gccatcccgc
      601 gctggcgcgc aaatctcaca ctgggtgaga actacatcaa ttctgagatt ttcagctcct
      661 ggcgctatac cggtgccagt ctggaaagtg atgaccgcat gctgccgcca agactgcgcg
      721 gttatgcacc gcaggtctca gggattgcgg atacgaatgc gcgcgtcgtg atttcgcagc
      781 agggacgcat cctctatgac gccaccgtgc cggcaggtcc gttcaccatt caggacctgg
      841 acagctccgt gcgtggccgc ctggatgttg aagttatcga gcaggacggg cggaaaaaaa
      901 cgttccaggt ggacacggca tatgtgccct atctgacccg tccgggccag gttcgttacc
      961 ggctggtttc cggtcgttcg cgtaactatg agcacaccac ggaaggcccg gtctttgcta
     1021 ccggtgaggc atcctggggt atcagcaaca agtggtctct ttacggtggc ggtatcgttg
     1081 ccggggatta caacgccctg gctgtggggc tgggccggga cctgaatgag ttcggtactg
     1141 tttccgctga tgtaacccag tccgtggccc gcattccggg agaagagacg aagcagggta
     1201 aatcctggcg tctgagctac tccaaacgtt ttgatgacgt gaatacggat atcacttttg
     1261 cgggttaccg cttctctgaa cgcaattaca tgacgatgga ccagtacctc aatgcccgtt
     1321 accggaatga cttcacggga cgtgagaagg agctgtacac cgtcacgctc aacaagaact
     1381 tcgaggactg gaagacctcg gtcaacctgc agtacagcca tcagacttac tgggaccgcc
     1441 ggacatctga ctattacacg ctgtcggtga accgctactt cgatgcgttc agctttaaga
     1501 atatcgcgct gggaatatct gcatcccgtt cgaaatacca gaaccgggat aacgattcgg
     1561 cgtttgttcg tctgtccgtg ccgtggggaa ccggtacggc cagctacagc ggcagcatga
     1621 gcaacgaccg ttacaccaat acggtgggtt acagcgacac gctgaacaaa gggctgagca
     1681 gttacagcct gaacgccggt gtgagcagtg gcggtggtca gccatcgcag agtcagatga
     1741 gcgcgtacta caaccacagc agtccgctgg ccagcctgtc agcgaacttc tctgctgtgg
     1801 agaacagtta cacgtcattc ggtatgagcg catccggtgg tgcaacgata accgcgaaag
     1861 gcgcggccct gcatgcaggg ggcatgaacg gaggaacccg tcttctggtg gataccgatg
     1921 gtgtcggagg tgtgccggtt gacggcggcc gggtctccac caaccggtgg ggcattggtg
     1981 tggtgacgga tgtgagcagc tattaccgca acaccacctc agtggacctg aacaggctgc
     2041 cggaagacat ggaagccacc cgctcggtgg tggaatcggt cctgacggaa ggggccattg
     2101 gttacaggga gtttgaagtc ctgaaaggaa gtcgtctgtt tgccgtgttg cggatggcag
     2161 acaacagcca tccgccgttt ggtgccagcg tgacaaacgc gaaaggccgt gagctgggga
     2221 tggtggcaga cagcggcctg gcctggctga gcggagtgaa tccgggtgaa accctgaatg
     2281 tcggctggga cggccggaca cagtgtgtgg tggacatccc ggcacatccg gacccggcac
     2341 aacagttgct gctgccctgt cgtcaggtga agtgacgtta tacacgccgg cgatcgtctg
     2401 tatatcccgg cagaaaaatg taaagaaaca gagagcagaa aatatgcaga aaatgaaacc
     2461 ggcgctgaaa aaaacgctga tggcggtagc gtgtctgagc gcagtaccgg cagcccaggc
     2521 tgccatctcc ctggaccgca cacgtgcgat ttttaatggc gatgagaaat cgatgacact
     2581 gaatatcgcc aatgataaca aacagcttcc ctatctggcc caggcatggg tggagaatga
     2641 aaagaaagaa aaaatcacca caggcccgat tattgccact ccgccagtac agcgtctgga
     2701 acccggctca aagagtatgg ttcgtctgac cagtacacct gacatcagcc gcctgccaca
     2761 ggaccgggaa tctctgttct atttcaacct tcgtgaaatt cctccgaaga gtgataaggc
     2821 aaatgtgttg cagatagccc tgcagacaaa aataaaactg ttttatcggc ctgaatctat
     2881 taaagcaaaa cccaacgcag tatggcagga ccagctggtg ctgaataaga ccggtggagg
     2941 gtatcagatt gataatccga caccgtatta tgtgacggta attggaattg gcggcggtga
     3001 aaaacaggcc agagaaggtg aatttgatgc tgtgatgctg gcaccaaagt caacgcagat
     3061 ggtgaaatca ggaacataca gcacacccta tctgagttat atcaatgact atggtggacg
     3121 tccggtactg cagttctcct gtgcaggaag tcgctgtact gcagtaaaga aataagaaag
     3181 cagtaaaacg aactgaccgg aggtgtcgga gatgaacagg gcaacaatag gattttatct
     3241 ggcagttgtg ctggggagtt gcagtatgaa cggtgtctca caggctgatg aattgttgac
     3301 gcgggatgat tttttcgtag cagatgaaag ccgccatcag tgggtaaatg agcacaacgg
     3361 aaggactgga gcgctgaatg tcaaaggggc attagtttca tctccgtgtc agctggatac
     3421 taatgaggtg caaatttcag aacggaagaa acgacgtgat atacagaaag ttaagatcaa
     3481 tatgtcaggt tgtggttatg gtaatgttcc ggagtcgttc tcagaatgga acagaaaatt
     3541 atatgta
//
LOCUS       DADSZD010000270         3506 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.272, whole genome shotgun sequence.
ACCESSION   DADSZD010000270 DADSZD010000000
VERSION     DADSZD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.272"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..443
                     /locus_tag="J1Z28_004329"
     CDS             <1..443
                     /locus_tag="J1Z28_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1795 domain-containing protein"
                     /protein_id="HBA7719086.1"
                     /translation="YLNETVVTFPGNIIQDSTINMLRLSDPDAALIISRGQMQEGDDL
                     TSQIEQQMKKLEKQVKDLHYTPVQVTRVGINDGEEGLEIQSQFLRGNEQVYQCQVAFV
                     LPGERVMMAFTYARTTPLTPADMTRWAEIKKNLRFRMRQEAGTN"
     gene            462..>3506
                     /locus_tag="J1Z28_004330"
     CDS             462..>3506
                     /locus_tag="J1Z28_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBA7719087.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLAASVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALADMLFPPQTEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEEARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVAGAAPGST
                     LTPDDKIDCHKHPSSLTQLLAQKKAAFLDDPPGTLLNLLSPDGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPAARSGARCTCEARVVNAPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVSTVALMFLRPGKMLSEIACFAAGIGMSILTQKAATA
                     LPHPVSAATGAKYLAGDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVRTPGSADENCMTFVSGMGRRLEMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QFFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRITEISGEQQRPCIRLHYELAA
                     HPRRVTQIHQHFPDTTPLLLRRYRYDGAGDLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWSLAARRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDAQGGKWRYVYDRQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNSWRCVYDERGNLLAVTDPLQQNTRYQYDRHGQVVQ
                     ITDARGGNKYLQWNEDGQLMRHTDCSGSQTAWFYDERTRLIRMTDAESHSTRYGYDDS
                     GHLTEVILADGRTVNYQSDAAGRLVKYTSPMGRITRWQRDGQGRVRSRTDATGRRTAF
                     GYDAYGRLVTLTNENGESYRFRHDVLDRLAEQINPDGCRQAYRYNALNAVTEVVFTGD
                     RGGEIRHRLARDAAGRLTAKETAGSRTEYVHDAADQLLEIRRR"
BASE COUNT          768 a          930 c         1192 g          616 t
ORIGIN      
        1 attatctcaa tgaaactgtt gtaacttttc ccgggaatat tattcaggac agtacgatta
       61 atatgctgcg cctcagcgat cccgacgctg cactcattat cagccggggc cagatgcagg
      121 aaggagacga cttaacctcc cagattgaac agcaaatgaa aaaactggag aaacaggtaa
      181 aggatcttca ctacacgccc gttcaggtaa cacgggtggg gattaatgat ggtgaagaag
      241 ggctggaaat acagagccag tttctccggg gtaacgagca ggtgtaccag tgtcaggtgg
      301 catttgtatt gccgggtgag cgggtcatga tggccttcac ctacgcccga accacaccgc
      361 tgaccccggc agatatgacg cgctgggctg aaattaaaaa gaatctgcgc ttcaggatgc
      421 ggcaggaagc cgggacgaat taatcacaca gggaacggga catggaagag gcattctggg
      481 cggcaagaca gggtgatgcc ctgctgcata cgtcgtttat ggcagatgta ctgggggcgg
      541 tgctggaggt ggccgcaaat gtcgttattg atgccctcat tgtcggcaca acctcgttac
      601 tggcggcgtc agtggggatc acgatggggt gcacggccgt cgtgctgacc ggttttgtgg
      661 cgggtgtggc gatggtgtac accggcgtga cagaaaaggt cagcgaagcc tgttcagccc
      721 tcgcagatat gcttttcccg cctcagactg aaggttacat cctcaccggc tctggtgaca
      781 catggattaa cagcaaacct gccgcgcggg cagcggcgac tgcggcatcg cggcaggaca
      841 ttgaggcgca ggaagcgcag gcgaaggcgg aacaggaaga agcacagcgt caggaggagg
      901 ccagaacgtt ccgggatgtg gccggggaat atctggaaat ggcgggggcc atagccctgg
      961 cagttaaccc ggtgacaggg cctgtgctga tgtcccgggc gctgcacgac caactcagta
     1021 cggaagaagg gcgttctgaa atggtggatg gcgtgacaca ttttgtgtcg gaactatggc
     1081 agcccacggt ggccggtgcg gctcctggct ccaccctgac tcctgacgat aaaatcgact
     1141 gccataaaca tccctcctct ctgacgcagt tactcgcaca aaaaaaagcc gcctttctgg
     1201 atgaccctcc gggaacgctc cttaatctgt tgagtccgga cggtatgctg gaaaccgcct
     1261 ttcagggagt gaatgcggtt attggcagca tcagtaatct gttcaaaggc gatgacgaac
     1321 cgcccgcggc ggaatatatc gcggaaggcg cgagggatgt gcgtatcaac agccagccgg
     1381 cagcacgcag cggggcacgc tgtacctgcg aggccagggt ggtgaatgcg ccggggaacg
     1441 gggcgtttgt gtcaccggat gtgcgcattg gcggtccgtt actggtggtc agggatatca
     1501 gaagcggcag gtcgcagata actctggtgt caaccgtggc gctgatgttt cttcgcccgg
     1561 gcaaaatgct ttctgaaatc gcctgttttg ctgccggtat cgggatgagc attctgacgc
     1621 agaaggccgc cacagccctt ccgcatccgg tcagtgccgc caccggggcc aaatacctgg
     1681 ccggtgatga tgacttcgat ttcagtctgc cgggccactt tccgctggac tggcagcgcg
     1741 tgtacagcag tcgtgacgaa cgcacggaag gcatgtttgg tcagggctgg agtgtaatgt
     1801 atgaagtcag cctcgtgcgc acaccgggta gcgcagacga aaactgcatg acctttgtgt
     1861 cgggtatggg acgacgcctg gagatggaag ccgtactgcc ggggggcggg ttttacagcc
     1921 ccggcgaagg actggcggta cggcggggtg agcaggggca ctggcttatc agcagcgatg
     1981 atggacagtt ttttctgttt gaggccgacc cgcaccaccc gcagcgccag cggctgaaaa
     2041 tgctgggcga ccgcaacagc aactgtctga atctgtacta cgacgaccgg ggacgcatca
     2101 ctgaaatcag cggcgaacag cagcgtccgt gcatccgtct gcattatgaa ctggcagcac
     2161 atccccggcg ggtgacacag attcatcagc atttccctga tacgacgcct ttactgctgc
     2221 gtcgttaccg ctatgacggg gccggcgacc tgaacggcgt gtacgacagc acggggcacc
     2281 tgctgcgtga gtttgcttac gacgaaaacc actgcatgac gctgcaccgc cagccgggcg
     2341 gcgaagggta ttattaccag tggggctggt atgaagggcc ggatgacgcg gcatggcggg
     2401 tgacggggca tcacacggac agcggtgaac agtaccggct ggcgtggagc ctggcggcac
     2461 gcaggctgtg cgtgacggac gggctggggc gcacgcgtta tcatcagtgg gacgcgcaga
     2521 atcaggtgac ggcgtaccag gacgaagccg gacaggtgac gacgttccgc tggagcgacg
     2581 aagaacggct gctgctggga atgacggatg cgcagggcgg caaatggcgc tatgtgtatg
     2641 accggcaggg gcatatcacg gagacgcacg acccgctggg acgggtggag caggcgcagt
     2701 ggcacccggt gtggcatcag ccggaaacgg aggtggacgc ggcgggaaac agctggcgct
     2761 gtgtgtacga tgaacgcggc aaccttcttg ccgtgaccga cccgctgcag cagaacaccc
     2821 ggtatcagta tgaccgccac ggtcaggtgg tgcagataac ggatgcgcgc ggggggaata
     2881 aatacctcca gtggaatgaa gacgggcagc tcatgcggca cacggactgc tccggctcgc
     2941 agacggcgtg gttctacgac gaacgcacgc ggctcatcag aatgacggat gcggagagtc
     3001 acagcacgcg ctacgggtat gacgacagcg gccacctgac ggaggtgata ctggcggacg
     3061 gccgcacggt gaattaccag tcggacgcgg cgggcaggct ggtgaagtac accagcccga
     3121 tggggaggat aacgcgctgg cagcgtgacg ggcagggccg ggtgcgcagc cggactgacg
     3181 cgacgggacg caggacggcg ttcgggtacg acgcttacgg gcgtctggtc acgctgacaa
     3241 acgagaacgg agaaagctac cggttccgcc atgacgtgct ggaccgtctg gcggagcaga
     3301 taaacccgga cggctgccgc caggcgtacc gctacaacgc gctgaacgcg gtcacggaag
     3361 tggtgttcac cggggaccgg ggcggtgaaa tccgtcaccg gctggcgcgt gacgcggcgg
     3421 gccggctcac ggcaaaggag acagccggta gccgtacgga atacgtccat gatgcggcag
     3481 accagctact ggaaatccgc cgccgg
//
LOCUS       DADSZD010000271         3477 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.273, whole genome shotgun sequence.
ACCESSION   DADSZD010000271 DADSZD010000000
VERSION     DADSZD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.273"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..861
                     /locus_tag="J1Z28_004331"
     CDS             <1..861
                     /locus_tag="J1Z28_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBA7719088.1"
                     /translation="VAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNA
                     LPRELKPLGQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSI
                     AEYQKTIAGNIEELENISRLTENILFLARADKNNVLVKLDALSLNKEVENLMDYLEYL
                     SDEKEIRFKVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNI
                     DIASPGTKINEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHN
                     VFRITLPQRN"
     gene            complement(969..1820)
                     /gene="hchA"
                     /locus_tag="J1Z28_004332"
     CDS             complement(969..1820)
                     /gene="hchA"
                     /locus_tag="J1Z28_004332"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBA7719089.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(2416..3474)
                     /locus_tag="J1Z28_004333"
     CDS             complement(2416..3474)
                     /locus_tag="J1Z28_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719090.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKVELYGKMVGERIL
                     TDRESGEKGDNSQDTSYARVGVKGETQINPELTGYGQFELDLEASNRHNPDQTRLAYA
                     GLSYKDFGSFDYGRNVGVAYDAEAFTDMFVEWGGDSWAGTDLFMTNRTNGVATYRNTD
                     FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSFAGAYANSDRTDWQS
                     GDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNKTQNFEAVLQYQFDF
                     GLRPSIGYSYSKALDVAGYKDSDRLNYIEIGTWYYFNKNMNVYTAYQINLLDKSDYVL
                     AHGLNTDDQLAVGIVYQF"
BASE COUNT          965 a          725 c          804 g          983 t
ORIGIN      
        1 gtcgccatta tattgtgctc agtattaagt ccgttgttaa tcagaacggg gttgcgagag
       61 atcaaaaagt tgagtggcgt aacggaagcg ctgaattata acgatagccg agtgccggtt
      121 gaggttaacg cattaccgag agaactaaaa cctcttgggc aggcgttgaa taaaatgcat
      181 catgctttag tcaaagattt tgagcgcctt agtcaatttg ctgacgatct cgctcatgaa
      241 cttagaacgc caattaatgc attactgggt cagaatcagg ttacgctcag tcaaaccaga
      301 agtatcgctg aatatcaaaa aacaattgcc ggaaacattg aagagctgga aaatatttcg
      361 cggttaacag agaatatact gtttcttgcc agggcagaca aaaacaatgt tttggtgaaa
      421 ctggatgcgc tttctctcaa taaggaagtt gaaaatctta tggactatct tgaatatctt
      481 tcagacgaga aagagattcg ttttaaggtc gagtgcaatc aacaaatctt tgcggataaa
      541 attttactgc aacgaatgtt atcgaatctt attgttaacg ccattcgata ttcaccagaa
      601 aaatcgcgta ttcatataac cagttttctt gatgccaacg gctctcttaa tattgatatc
      661 gccagtcctg gaacgaaaat taatgagcct gaaaaactct tccgtagatt ttggcgggga
      721 gataattcgc gtcattccgt aggtcaggga cttggccttt ctttagtcaa agcgattgcc
      781 gaattacatg gaggaagtgc tacgtatcac tatctcagta agcataacgt gttccgaatt
      841 acgttaccgc aaagaaatta acatcgggct tcagtacgcc ataagttaac cgtgaaaaaa
      901 cgcagccacg tcgtgagtat taacgtggct gcgattgatt atgcgcttac attacaatgt
      961 aacagggatt aactcgcgta agctgccagc atttcctgcg ccgccagttt acccaacgca
     1021 ttcgctgcaa aaggactgtc gccggtgaga agtttacggt ccttatgtac tcgcccggtg
     1081 atgtcgtcat taatgatgtt catgcccatt ttcttcaact cttcgccgaa gtaccaggtg
     1141 agatgacccg gcatatagcc aatatctggc gtttgtttgt ctgcggcgtc tgggaatgcg
     1201 caaatagaat aaccattcag tgggttatcg ctgtggcgaa gtgccagaaa agccgccgga
     1261 ccgtggcaaa gggagataac aaaacggtca tttttgattg cccactgtaa agcggcagcc
     1321 acgtcctggc tttctggtaa accaataagt gcgccatgcc caccaggaac aaagattgct
     1381 gcatattcgc tatcagcgtt gaggctggcg acaacatccg cgagtttctt cggattacgg
     1441 aataacgatt tatgctgttc aaagaatggc attacttttt catctttgtg cggcatagcc
     1501 cagtattcaa acttggtcat cagaccggaa atggtcgcca cttcgaattc gaaacctgca
     1561 gcatggagat gatacaacgg cagcaacgtt tcaatcggat ggttaccggt cgagaacagt
     1621 tttccgttat cggtcggcaa ataacgttcg tccgcggcga tcaccagaat tttatgttta
     1681 ccgcgatacg gttttggata gtccacgcca tcaagatcag agacgggact cgtatattgg
     1741 ctaagcgaat attctgaagg gaagaacgca ttatcttcag caatatcgac ctgcgggttt
     1801 ttacttgttt gaacagtcat agtgtattcc ttattgttgc ttagttaggg tagtcactat
     1861 atttgcgact gagctggtcg ctatttttaa ccaggctgaa aaattgagtc aatatatcga
     1921 aagcggtagt tggcgggcat aagtatgtaa tgatgatgct gcactggtgt tgaatattat
     1981 tcacgccaaa ctttccttat tgagtaacgt cgtcgcaagt ggcctcaatg attttagacc
     2041 attatcagag gtttgggggc gatggggaga gatttagtgc tgtggaatgt tgtggtactg
     2101 atgaatattt atatcccgga ttggtaggga aaaagctttt ttcatgcact atttttgcaa
     2161 atatttctat atgggaagcg gttttgttac agattaaatt tataaagaaa tgcatttggg
     2221 gtgttggctg gaagagttgc tggtagagga tattgtgcca ggaggggcag ggtaaggggc
     2281 tgtatcgaga tgctttggca attgcgtgtg ccgcagatgg agatgaaatg ccaataaaaa
     2341 aagcctgagt tcgatgaggg acgaactcag gctggtgtta tgttttacag taacacgcct
     2401 cggcgcgttg gctgattaga actggtagac gataccaact gccagttggt cgtcggtgtt
     2461 cagtccatga gcgagaacat aatcactctt atcaagcagg ttgatctgat aagcggtata
     2521 tacgttcata ttcttattga agtaatacca ggtaccgatt tcaatatagt tcagacgatc
     2581 agaatctttg taacctgcta cgtccagtgc tttagaatag gagtaaccga tggatggacg
     2641 cagaccaaag tcgaactgat attgcagaac agcttcgaag ttttgtgttt tgttcacaac
     2701 atcaccgtcg tcggaattca tgttgtgaga ttcaccgtac atcacagcag catagacatt
     2761 gttagcgtca tattttgttg acaacgccca aacttcggct ctttcgcctt taccatcacc
     2821 agactgccaa tcagtacggt cagagtttgc ataagcacca gcaaatgaga aaccgtcaat
     2881 agtgtaagtt gcagataaac catggccatc gccatttgct ttgtagttgc ctgtaccttc
     2941 atttttaccc tgatattgca gagcaaaatt tagaccttca accatgccaa agaaatctgt
     3001 gttacgatag gttgcaacac cgttggtacg gttggtcatg aacaggtcag taccagccca
     3061 tgagtcaccg ccccactcaa caaacatatc agtaaatgct tctgcatcat acgcgacacc
     3121 gacattacga ccgtagtcga atgaaccaaa atctttgtaa ctcaaaccag catatgccaa
     3181 acgagtttgg tcagggttgt gtctgtttga tgcttccagg tctagttcaa actgaccgta
     3241 accagtcagt tcagggttga tctgagtttc acctttcacg cctacacgag cataagaggt
     3301 atcttgagaa ttatcgcctt tttcaccact ttcacggtcg gtcagaatgc gctcaccaac
     3361 catcttgccg tatagctcaa ctttattgcc atttttatta taaatttcag ccgcatttgc
     3421 tgcgccagca actaataacg ccgggaccag cattgccaga acttttcttt tcattat
//
LOCUS       DADSZD010000272         3464 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.274, whole genome shotgun sequence.
ACCESSION   DADSZD010000272 DADSZD010000000
VERSION     DADSZD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.274"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            191..1156
                     /gene="dusB"
                     /locus_tag="J1Z28_004334"
     CDS             191..1156
                     /gene="dusB"
                     /locus_tag="J1Z28_004334"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBA7719091.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1182..1478
                     /gene="fis"
                     /locus_tag="J1Z28_004335"
     CDS             1182..1478
                     /gene="fis"
                     /locus_tag="J1Z28_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBA7719092.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1564..2448
                     /gene="yhdJ"
                     /locus_tag="J1Z28_004336"
     CDS             1564..2448
                     /gene="yhdJ"
                     /locus_tag="J1Z28_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBA7719093.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2532..2711
                     /locus_tag="J1Z28_004337"
     CDS             2532..2711
                     /locus_tag="J1Z28_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 domain-containing protein"
                     /protein_id="HBA7719094.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(2714..3376)
                     /gene="acrS"
                     /locus_tag="J1Z28_004338"
     CDS             complement(2714..3376)
                     /gene="acrS"
                     /locus_tag="J1Z28_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBA7719095.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDYLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMACYDLYKQAPALVDNVLRMFMPDENITKLIHKTNELS
                     VI"
BASE COUNT          956 a          790 c          811 g          907 t
ORIGIN      
        1 cttggagtgt aaattttagt cactattttc taatataatg atttttatga gtaattatcg
       61 cgccaagctc attttaaatg caattctttg atccatctca gatgattggt caaagtttgg
      121 cctttcatct cgtgcaaaaa atgcgtaata tacgccgcct tgcagtcaca gtatggtcat
      181 ttcttaactc atgcgcatcg gacaatatca gctcagaaat cgcctgatcg cagcgcccat
      241 ggctggcatt acagacagac cttttcggac gttgtgctac gagatgggag ccggattgac
      301 agtatccgag atgatgtctt ctaacccaca ggtttgggaa agcgacaaat ctcgtttacg
      361 gatggtgcac attgatgaac ccggtattcg caccgtgcaa attgctggta gcgatccgaa
      421 agaaatggca gatgcagcac gtattaacgt ggaaagcggt gcccagatta ttgatatcaa
      481 tatgggttgc ccggctaaaa aagtgaatcg caagctcgca ggttcagccc tcttgcagta
      541 cccggatgtc gttaaatcga tccttaccga ggtcgtcaat gcagtggacg ttcctgttac
      601 cctgaaaatt cgcactggct gggcaccgga acaccgtaac tgcgaagaga ttgcccaact
      661 ggctgaagac tgtggcattc aggctctgac cattcatggc cgtacacgcg cctgtttgtt
      721 caatggagaa gctgagtacg acagtattcg ggcagttaag cagaaagttt ccattccggt
      781 tatcgcgaat ggtgacatta ctgacccgct taaagccaga gctgtgctcg actatacagg
      841 ggcggatgcc ctgatgatag gccgcgcagc tcagggaaga ccctggatct ttcgggaaat
      901 ccagcattat ctggacactg gggagttgct gcccccgctg cctttggcag aggttaagcg
      961 cttgctttgc gcgcacgttc gggaactgca tgacttttat ggtccggcaa aagggtaccg
     1021 aattgcacgt aaacacgttt cctggtatct ccaggaacac gctccaaatg accagtttcg
     1081 gcgcacattc aacgccattg aggatgccag cgaacagctg gaggcgttgg aggcatactt
     1141 cgaaaatttt gcgtaaacag aaataaagag ctgacagaac tatgttcgaa caacgcgtaa
     1201 attctgacgt actgaccgtt tctaccgtta actctcagga tcaggtaacc caaaaacccc
     1261 tgcgtgactc ggttaaacag gcactgaaga actattttgc tcaactgaat ggtcaggatg
     1321 tgaatgacct ctatgagctg gtactggctg aagtagaaca gcccctgttg gacatggtga
     1381 tgcaatacac ccgtggtaac cagacccgtg ctgctctgat gatgggcatc aaccgtggta
     1441 cgctgcgtaa aaaattgaaa aaatacggca tgaactaatt caggttagct aaatgcttga
     1501 ttaaaaaggc gctactcggc atggggaagc gcctttttta taggtgtcac aaagggagtg
     1561 accatgagaa caggatgtga accgacccga tttggtaatg aagcaaagac catcatccag
     1621 ggtgatgcat taaccgaact taaaaagctc cccgccgaaa gtgtcgatct gatctttgcc
     1681 gacccaccgt ataacatcgg taaaaatttt gatggtctga tcgaagcctg gaaagaagat
     1741 ctgtttatcg actggctgtt tgaagtgatt gcagagtgcc atcgcgttct gaaaaagcag
     1801 ggcagcatgt acatcatgaa tagcacggaa aacatgccct ttatcgatct ccagtgccgc
     1861 aagcttttta ccatcaaaag tcgcatcgtc tggtcatatg acagttctgg agtgcaggcg
     1921 aaaaaacact acggctccat gtacgaaccc atcctgatga tggtgaaaga cgcaaagaac
     1981 tacacgttca acggtgatgc tattctggta gaagctaaaa ccggatcgca gcgcgcgttg
     2041 atcaattatc gcaaaaatcc tccacagcct tacaatcatc aaaaagtacc gggtaacgtc
     2101 tgggattttc cgcgcgtgcg ttatttgatg gatgaatatg aaaaccaccc gacgcaaaaa
     2161 ccggaagcct tgctgaaacg cattattctc gcctcttcca acccaggcga tatcgttctc
     2221 gacccgtttg ccggtagctt tactaccggt gccgtagccg tcgccagcgg gcgaaaattc
     2281 attggtattg agatcaacag cgagtacatc aaaatggggc ttcgacggct ggatgtcgcg
     2341 tcgcattact ctgcggaaga actggcgaaa gtgaaaaaaa gaaagaccgg caacctgtca
     2401 aaacgaagcc ggttaagcga agttgacccc gatctcattg caaagtaaag gaagtgtaag
     2461 cctgtttttc agattattca tttcgtgtat atttcctgcc agacttggtt aaacatgcac
     2521 aggctcaggt aatgattcgc aagtattggt ggctcgtcgt tttcgctgtc ttcgtttttc
     2581 tgtttgatac tttactgatg cagtggattg aactgctggc aacagaaaca gacaaatgcc
     2641 gcaatatgaa ctcagttaat ccactaaaac tggttaactg tgacgaactg aattttcagg
     2701 acagaatgtg aatttatatg acacttaatt cattcgtttt atgaattaat ttcgttatgt
     2761 tttcatctgg catgaacatt cttaatacgt tatcgaccag tgcgggtgct tgtttataaa
     2821 ggtcataaca cgccatattc attaaccagt tttgaacaat tccgctgaag gcaccatcaa
     2881 taataatcat cacaacatct aaatcgagat tatttgctac acaaccttgt tgctgacacg
     2941 cctgcaatac ttcgcggaga gtctgcggat taaagcccat cttttcgcgt atcactccct
     3001 cggccagcat ctcatcatta aattcacatt tgtgatataa gattttcagc aacgcctgct
     3061 ggcggggaat tttggcaata tattgcaaac cgacaatcaa tttttcacgc aattgctgaa
     3121 acgggtcatg ctctaaccca gcggtcaagt aatcctggat taactcccgc aatgaaggct
     3181 gttgcaacca catctcatta aacagctgag tcttgttttc gaagtgccag tagatagcgc
     3241 cacgcgtaac gttagcagcg tcggcaatgt cgttgagcgt cgtcttgctt acgccatgct
     3301 gcgcaaactg ggcgatggca gtttcaatca gttcttgccg ggtcttcagg gcttcagctt
     3361 tggttctttt tgccatgatt aattattcag gaaataaata tattcgacac aggatgagaa
     3421 aatagcgaag gttaatctat cacctaatgt gtatttatac gaga
//
LOCUS       DADSZD010000273         3430 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.275, whole genome shotgun sequence.
ACCESSION   DADSZD010000273 DADSZD010000000
VERSION     DADSZD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.275"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..309
                     /locus_tag="J1Z28_004339"
     CDS             <1..309
                     /locus_tag="J1Z28_004339"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719096.1"
                     /translation="YRSESRLTLWQHHSLRILWCYYHQVVKSFCNFVKYFFPLDREDL
                     LNLMDLTKNSPDTKQDLLDHLLSGKRMTGRDEMDIKRILKTKEALNNIRKFLDKKGNK
                     "
     gene            complement(385..1584)
                     /gene="lolC"
                     /locus_tag="J1Z28_004340"
     CDS             complement(385..1584)
                     /gene="lolC"
                     /locus_tag="J1Z28_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBA7719097.1"
                     /translation="MYKPVTVFIGLRYKYGSAADRFSRFVSWLSTIGITLGVMALITV
                     LSVMNGFERELQKNILDLMPHAILSAEKGYINDDILPADRIKLEGIQRIAPLTTGEVV
                     LQSAGNVAAGIMLGIGPAEKDPLSPYLVNVSQSVLQPGEYNVILGEQIAAQLGVNRGD
                     KVRIMVPSASQFTPLGRLPAQRLFTVAGIFAAGSDIDKSELLVNIQDASRLMRYPSGS
                     FSGWRLWLTAPLQVGELSRQTLPDGITWTDWRERKGELFQSQRMEKNIMGLLLSLIVI
                     VAAFNIITSLGLMVMDKQAEVAVLQTQGLTSRQVMMVFIVQGVSTGVIGALVGAVSGI
                     VLAGQLNNLMPVTGLLTGNIELPVATEPLQVIFVVLATILISLLSTLYPAWKAASIPP
                     AEALRYS"
     gene            complement(1712..2608)
                     /locus_tag="J1Z28_004341"
     CDS             complement(1712..2608)
                     /locus_tag="J1Z28_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003036300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7719098.1"
                     /translation="MKENLNDLFTFLTVVREGSFTRAAAKSGMSQSAVSQTIGNLEAR
                     LQLKLLNRTTRSLTLTEAGERLIQLTGPALEEIVVGLEQLKELRDKPAGRIRLSADEY
                     AIQYFLWPKLAPVLKEYPDIHLELITDYGLIDIAREGFDAGVRRGGLIARDMVAIPVS
                     AEHRMVVVISPQCLPEQDLPVVPSQLLKLPCINLQLPTHGQNFPWTFLINGEEQKLRV
                     QGQIVVNSISQVRQACLDGYGLAYVPQELVQNDITSGRLLTVLDDFSVTFPGYYLYYT
                     SRLKSSAAFNIIVESLRMSDLP"
     gene            2738..>3430
                     /locus_tag="J1Z28_004342"
     CDS             2738..>3430
                     /locus_tag="J1Z28_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003036296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7719099.1"
                     /translation="MKYTLITGASSGIGKALCYQFASRGYNLIITARREEELQKLRQD
                     IESQYAVHVIVKPCDLAQEGQAESLYHQLADFDIAVLINNAGFGDFSMPWDADLAKVK
                     RMLDLNVKALTTLSLLYTHDYADRDATLINVSSIGGYSIFDIAVTYCATKFFVASFTE
                     GLAQNLRDQGKKMRAKVIAPGPTQSEFITRSSDKGSICGDGLFAETSFLTAEKLADYI
                     WQLSESDSTVGII"
BASE COUNT          937 a          849 c          765 g          879 t
ORIGIN      
        1 taccgttcag aaagccggct gacgctgtgg caacatcaca gtctgagaat tctctggtgc
       61 tactatcatc aggtggtaaa aagtttctgt aattttgtaa aatacttctt tcctctcgat
      121 cgtgaagatt tgcttaacct gatggatctc accaaaaatt ctcctgatac gaaacaggat
      181 cttcttgacc atcttttgtc cggaaaaaga atgaccgggc gggatgaaat ggatatcaag
      241 cgcattctaa aaacaaaaga agcacttaat aacatcagga agtttcttga taaaaaggga
      301 aataaatgaa aaagaggggt atccatcccc ccctctgatt atatccgaca gacattaatc
      361 atctgaatcg ttccggtcag tgaattatga gtaccgtaat gcctctgcag gcggtatgga
      421 tgctgctttc catgccggat acagtgtgga caataaagag atgagtattg ttgccagaac
      481 gacgaaaatt acctgcaatg gttctgtggc tacgggtaac tcgatatttc cggtcagtaa
      541 ccccgttacc ggcatcaggt tatttaactg gcctgcgagt actatacctg atactgcgcc
      601 aaccagcgca ccgatgacac ctgtactgac tccctgaaca atgaaaacca tcattacctg
      661 acgtgaagtt aatccctgcg tctgcagtac cgccacttcg gcctgtttat ccatcaccat
      721 aagtccaaga gaagtgatga tattaaatgc ggcaacaatc acgataagac tgagtaaaag
      781 ccccatgatg tttttctcca tccgctggga ctggaataac tctccttttc gctcacgcca
      841 gtctgtccat gttattccgt ccggcagagt ctgacggctg agttctccca cctgcaatgg
      901 ggctgtcagc cacagacgcc atccggaaaa actccccgaa ggataacgca taaggcgtga
      961 cgcatcctgt atatttacca gtaactcact cttgtcaatg tcactgccgg cagcaaaaat
     1021 tcctgcgaca gtaaacaggc gctgagcagg caaacgaccg agtggagtga actgactggc
     1081 tgacggaacc ataatgcgga ctttatcacc gcgattaacc cccaactgag ccgcaatctg
     1141 ttcaccaaga atgacattat actcacccgg ttgtaatacg gactggctca cattgaccag
     1201 ataaggtgac agagggtcct tttctgccgg cccgataccc agcataattc cggcagccac
     1261 attgccggca ctttgcagca caacctcacc tgttgttaag ggtgcaattc gctgaatacc
     1321 ttcaagcttt atcctgtctg caggtaatat gtcgtcatta atataacctt tctctgcaga
     1381 gagaatggca tggggcatca ggtccagaat attcttctgt aattcacgtt caaatccgtt
     1441 cattacagat aatactgtta tcagggccat aacgccgaga gtaataccaa tcgttgaaag
     1501 ccaggataca aatcggctga agcggtctgc tgcgctacca tatttatagc gaagccctat
     1561 aaaaacagtt acaggtttgt acatatctct tttttaacaa cggttactaa tcagaattgt
     1621 tcatacgccg gccagcaggt aataaccggc aggataattg tcatcagtta acgaatttat
     1681 gctcctgtat atgggctacg ctaccggttt atcagggcaa atcagacatt cgcagtgatt
     1741 cgacaataat attaaatgcc gcagaggatt tcaggcgact ggtataatac agataatagc
     1801 ccgggaaagt gactgaaaaa tcgtccagga cagtcagcag tcttcctgat gtaatatcat
     1861 tctgcaccag ttcctgtgga acatacgcca gaccgtaacc atcaagacag gcctggcgaa
     1921 cctggctgat actgttaacg acaatctgtc cctgaacacg taacttctgc tcttctccgt
     1981 taattaaaaa tgtccacgga aaattctgtc catgcgtggg taactgcaga ttaatacacg
     2041 gtaacttcag taattgcgaa ggaacaacgg gtaagtcctg ttccggaagg cactgaggcg
     2101 aaatcaccac caccatccgg tgttccgcgc tgaccgggat ggctaccata tcccgggcta
     2161 tcaggcctcc ccgtcgcaca ccggcatcaa aaccctccct ggcaatatca atcagaccat
     2221 aatccgtaat tagctccagg tggatatcag gatattcttt cagaaccggt gccagttttg
     2281 gccacagaaa atactgaatg gcgtattcat cggccgataa gcgtattctg cctgcaggtt
     2341 tatcccgcag ctctttcagc tgttcaagtc cgactacgat ttcttcaagt gccggccctg
     2401 tcagctgtat caggcgttcg ccagcttctg tcagtgtcag gctccgggtt gtgcgattga
     2461 gtagttttaa ctgcagacgc gcttcaaggt tgccgattgt ctggctcacc gctgactggg
     2521 acatccctga tttcgccgca gcacgtgtga agcttccctc ccggacaacc gtcagaaaag
     2581 tgaacaggtc gttaagattc tctttcatta atttataagt caggctgata acagacataa
     2641 gattatcctc acttatcaaa tgaactgtcc agccttatca tcaccgcatt atcacgacac
     2701 attgcccgtc aggctgcaac agtcaggaga acaacacatg aaatataccc ttattaccgg
     2761 agccagttca ggtatcggca aggctctctg ctaccagttt gcttcccgtg gttacaacct
     2821 gattatcact gcacgacgcg aggaagagct gcagaagctt cgacaggata ttgaaagtca
     2881 gtatgcagta catgtcatcg taaaaccctg tgatctggcg caggaagggc aggcagaaag
     2941 cctttatcat cagctggctg attttgatat tgccgtgctg atcaataatg cgggctttgg
     3001 cgatttctct atgccctggg atgccgattt agcaaaagtg aaacggatgc tggatctgaa
     3061 tgttaaggcc ctgaccacac tttccctgtt gtacacacac gattatgccg atcgggatgc
     3121 gaccctgatt aatgtctctt ccattggtgg ctattcaatt tttgatattg cagttactta
     3181 ctgcgcaaca aaattctttg tggcgtcatt tacagaaggg ctggcgcaga atctgcgtga
     3241 tcagggtaaa aaaatgcggg caaaagttat cgccccggga ccaacacaga gtgaatttat
     3301 cacccgttcc tcagacaagg gaagcatctg tggtgacggt ctgtttgcag agacgtcttt
     3361 tcttactgct gaaaaactgg cggattacat ctggcaactg tctgaaagcg acagtaccgt
     3421 aggcatcatc
//
LOCUS       DADSZD010000274         3362 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.276, whole genome shotgun sequence.
ACCESSION   DADSZD010000274 DADSZD010000000
VERSION     DADSZD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.276"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            346..1662
                     /gene="shiA"
                     /locus_tag="J1Z28_004343"
     CDS             346..1662
                     /gene="shiA"
                     /locus_tag="J1Z28_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBA7719100.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1764..3218
                     /gene="amn"
                     /locus_tag="J1Z28_004344"
     CDS             1764..3218
                     /gene="amn"
                     /locus_tag="J1Z28_004344"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBA7719101.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          784 a          816 c          807 g          955 t
ORIGIN      
        1 caatacgtct caacaatgat actaaaaaca taactaacct cgcctcccta ctggttataa
       61 tgcaatgcag tctatcagaa tcatcagggt gcaattttgt gcatatgcgg acttttatgt
      121 ttcatatctc gaacctgtgg gccctctgct taatccttaa acaacaccag caactcctgc
      181 gctttcatct tccatcgaat ttttcatgtt gccgctaatc agccataaaa tcatttgcag
      241 atgcgctctg tcgaggtagt ctcataaggt tcgtttatag atcgacggca atgtgagtta
      301 ccttttccat actaattata aaaagacagt acaaacagga tcattatgga ctccacgctc
      361 atctccactc gtcccgatga agggacgctt tcgttaagtc gcgcccgacg agctgcgtta
      421 ggcagtttcg ctggtgccgt cgtcgactgg tatgattttc tactctatgg cattaccgcc
      481 gcactggtgt ttaatcgcga gtttttcccg caagtaagcc cggcgatggg aacgctcgcc
      541 gcatttgcca cctttggtgt cggcttcctt ttccgaccgc tcggcggtgt cattttcggt
      601 cactttggcg accgactggg acgtaagcgc atgttaatgc tgaccgtctg gatgatgggc
      661 atcgcgacag ccttaattgg tattcttcct tcattctcga ccattgggtg gtgggcacct
      721 attttgctgg tgacactgcg tgccattcag ggatttgccg tcggcggcga atggggaggc
      781 gcagcgttgc tttccgttga aagtgcgccg aaaaataaaa aagcctttta cagtagcggt
      841 gtacaagttg gctacggtgt aggtttactg ctttcaaccg gactggtttc attgatcagt
      901 atgatgacga ctgacgaaca gtttttaagc tggggctggc gcattccttt cctgtttagc
      961 atcgtactgg tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt
     1021 gaacaacagc aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg
     1081 ttacgacatc ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg
     1141 atgtacattg ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc
     1201 gaacttttcc ttaatattgg tttgctggta ggtggattaa gctgcctgac aattccctgt
     1261 tttgcctggc ttgccgatcg ttttggtcgg cgcagggttt atattacagg cgcgttgatc
     1321 ggaacgttga gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata
     1381 gttttcttct ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa
     1441 ccgatgttta ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat
     1501 caggttgcca gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact
     1561 tactttgccg ggaactggca tagcgtcgcc atttatttgc tggctggatg cctgatttct
     1621 gcaatgaccg ctttattgat gaaagacaat caacgctcgt gatagcctgg cgaagatcat
     1681 ccgatcttcg ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta
     1741 attgtatgga acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg
     1801 cactggataa actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aacgccattg
     1861 gcaaatatat tacaagtggc gaattacctg atgaaaacgc ccgcaaacaa ggtctttttg
     1921 tctatccatc actgaccgta acctgggacg gtagcacaac taatcccccc aaaacgcgcg
     1981 catttggtcg ttttacccac gcaggcagct acaccaccac gattactcgc cctactctct
     2041 ttcgttcgta tctcaatgaa caacttacgt tgctgtatca ggattatggt gcgcatatct
     2101 cggtgcaacc ctcgcagcat gaaatccctt atccttatgt catcgatggc tctgaattga
     2161 cacttgatcg ctcaatgagc gctgggttaa ctcgctactt cccgacaaca gaactggcgc
     2221 aaattggcga tgaaactgca gacggcattt atcatccaac ggaattctcc ccgctatcgc
     2281 attttgatgc tcgccgggtt gatttttccc tcgcacggtt gcgccattat accggtacgc
     2341 cagtagaaca ttttcaaccg ttcgtcttgt ttaccaacta cacacgttat gtggatgaat
     2401 tcgttcgttg gggatgcagc cagatcctcg atcctgatag tccctacatt gccctttctt
     2461 gtgctggcgg gaactggatc actgccgaaa ccgaagcgcc agaagaagcc atttccgacc
     2521 ttgcatggaa aaaacaccag atgccagcat ggcatttaat taccgctgat ggtcagggta
     2581 ttactctggt gaatattggc gtgggaccgt ccaatgctaa aaccatctgc gatcatctgg
     2641 cagtgctacg cccggatgtc tggttgatga ttggtcactg tggcggatta cgtgaaagcc
     2701 aggccattgg agattatgtc cttgcacacg cttatttacg cgatgaccac gttcttgatg
     2761 cggttctgcc gcccgatatt cctattccga gcattgctga agtgcaacgt gcgctttatg
     2821 acgctaccaa gttagtcagc ggcaggcctg gtgaagaagt caaacagcgg ttacgtactg
     2881 gcacggtggt aaccacagat gacaggaact gggagttacg ttactcagct tctgcacttc
     2941 gttttaactt aagccgggcc gtagcaattg atatggaaag tgcaaccatt gccgcgcaag
     3001 gatatcgttt ccgcgtgcca tacgggacac tactgtgtgt ttcagataaa ccgttacatg
     3061 gtgagattaa gcttcccggc caggctaacc gtttttatga aggcgctatt tccgaacacc
     3121 tgcaaattgg cattcgggcg atcgatttgc tgcgcgcaga aggcgaccga ctgcattcac
     3181 gtaaattacg aacctttaat gagccgccgt tccgataata agttatgaac cttcttatca
     3241 gaaatagatg tgaaggatta agtaagacat ctggcaaata gcctgcaata aacgcgggct
     3301 gtttgcttcc ttgggcagat gcgagtttta ttatcgtctt aatgatttcc acatattaaa
     3361 ag
//
LOCUS       DADSZD010000275         3353 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.277, whole genome shotgun sequence.
ACCESSION   DADSZD010000275 DADSZD010000000
VERSION     DADSZD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.277"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(1..756)
                     /locus_tag="J1Z28_004345"
     CDS             complement(1..756)
                     /locus_tag="J1Z28_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7719102.1"
                     /translation="MVVFSQQPFSFDGIKPWLYIWTMKTTLSERLKEARLARGLTQKA
                     LGDLVGVSQAAIQKIETGKANQTTKIVEIANALGVRAEWLSSGVGNMSDSTVQPILST
                     VSHSKYFKIDVLDIEVSAGPGVINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINV
                     RGDSMSGTIEPGDLLFVDITVKSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKS
                     YSPWDPIEKDEMNRVFIFGKVIGSMPQTYRKHG"
     gene            795..1025
                     /locus_tag="J1Z28_004346"
     CDS             795..1025
                     /locus_tag="J1Z28_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7719103.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            1095..1634
                     /locus_tag="J1Z28_004347"
     CDS             1095..1634
                     /locus_tag="J1Z28_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBA7719104.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAEDN
                     QDVVATLIVNEYREQGGGAIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEMSEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            1721..2650
                     /locus_tag="J1Z28_004348"
     CDS             1721..2650
                     /locus_tag="J1Z28_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HBA7719105.1"
                     /translation="MANTAEIFNFPVPDVAQKEPRVADLDDGYTRIANELLEAVMLAG
                     LTQHQLLVFLAVMRKTYGFNKKLDWVSNEQLSELTGILPHKCSAAKSVLVKRGILIQS
                     GRNIGINNVVSEWSTLPESGKKNKVYLKEVNLPESGKKSLPKSGKGVYPNQVNTKDKL
                     TKDNIKPFSSENSDESSDQPENDLPVVKSDAAIQSGSKWGTAEDLTAAEWMFDMVKTI
                     APSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWT
                     QLEINRNKQQAGVTAGKSKLDLTNTDWIYGVDL"
     gene            2647..3348
                     /locus_tag="J1Z28_004349"
     CDS             2647..3348
                     /locus_tag="J1Z28_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein P"
                     /protein_id="HBA7719106.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQLVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREETSVTAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELACMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGAMK"
BASE COUNT          939 a          811 c          829 g          774 t
ORIGIN      
        1 ctacccatgc ttcctgtagg tctgcggcat gctcccaata actttcccga agataaacac
       61 ccggttcatc tcgtctttct caattgggtc ccacggcgag tagctcttgt tatcagagat
      121 aaccagtagc ttatccttca tcatttgcag gcgctttaca tgggctgtgt cgtcgtacag
      181 aaacgcatag ataccatcac cgtcgaaaga tttaactgtg atatcaacga acagcagatc
      241 acctggttcg atcgttcctg acatgctgtc accacgcacg ttaatgatgc ggatattttc
      301 cgccttccta ccatcgaaca tgtgacgagc atcgtcaaac gagtactcaa ccgagcgtag
      361 aacttctaca aactcacggt tgatgactcc cggcccagca ctgacttcta tatcaagaac
      421 gtcaatctta aagtatttgg aatggctgac agttgatagt attggttgca ctgtactgtc
      481 tgacatattt ccaacgccag aagataacca ttctgcgcgc acacccaaag cgttcgcgat
      541 ctccacgatt ttagttgttt ggttagcttt ccctgtttcg attttctgaa tagcagcttg
      601 gctaaccccg accaaatccc caagcgcctt ttgtgtaagg cctcgcgcta atctggcttc
      661 tttaagtctt tctgagagtg ttgttttcat agtccaaatg tacaaccaag gttttattcc
      721 atcaaacgaa aatggttgtt gactaaaaac aaccatagtt ttaatcttga ttcaaattaa
      781 ccacggaggt tgttatgaac ccagctatca aaacagcgat caatatcgtt ggttcacaaa
      841 agaaactggg cgctgcctgc gaagtttcac agcaggccgt ctataagtgg cttcacaaca
      901 aagcaaaggt atcccctgaa catgtcggca gcattgttac ggctactggt ggagtagtga
      961 aggcatacca gattcgcccg gatcttccga agttgtttcc acacaccgaa aagaacgcag
     1021 cttaaatttc catttcacgc tctttaacaa taagcaatca acttaacagt caattcaaac
     1081 taaaggagtc aattatgcaa ccacttacat accaacagac tagcggattt agcccgactg
     1141 cggtgataaa tcgttctcaa acaaaacagg tgccaggcca cgaaaaaatc cgtgatgccg
     1201 tccgcgcctg gtcggctgaa gataatcagg atgtcgttgc cacactcatt gtgaatgagt
     1261 atcgggagca gggcggcggc gccatcgatt tccctgatga tgtcagccgt gcacgccaga
     1321 agctgttccg cttcctcgat aacaaattcg attctgaaaa ataccgaaat aacgtgcgtg
     1381 aactgacccc ggcaattctg gcggtactac cgctggaata tcgcggttac ctggttgagc
     1441 aggatagctt catggctagg ttggctgaaa tggaaaagga actcagtgag gcaaaacagg
     1501 ctgtcattct caacgcacca cgccaccaga aactgaagga gatgagtgaa ggtattgtgt
     1561 cgatgtttcg tgtggacccg gatctggctg gtccattgat ggcgatggtt accaccatgt
     1621 tgggggcaat atgacaggct cagaaatggc gaaagccggt ctgcgggaac agagccgact
     1681 ttcaggtgca aatcgtaaca cactcattgc gggaggaatt atggcaaaca ctgctgagat
     1741 attcaatttt ccagtgccgg atgtggcaca aaaggagccg cgcgtggcag atctcgacga
     1801 tggttatacg cgcattgcaa atgagttgct ggaagctgtg atgctggccg gattaacaca
     1861 gcaccagctt ctggtcttcc tggctgtcat gcgcaaaaca tatggcttta ataaaaaact
     1921 ggattgggtg agcaacgagc aactttccga attgaccggg atattgccgc acaagtgttc
     1981 tgctgcaaaa agcgttctgg taaagcgtgg gattcttatt cagagcgggc gaaatatcgg
     2041 cattaataat gtggtcagtg aatggtcaac attacccgaa tcaggtaaga aaaataaagt
     2101 ttacctgaaa gaggtaaatt tacctgaatc aggtaagaaa agtttaccca aatcaggtaa
     2161 aggcgtttac ccgaatcagg taaacacaaa agacaaacta acaaaagaca atataaaacc
     2221 tttttcgtcc gagaattctg acgaatcctc tgaccagcca gaaaacgatc ttcctgtggt
     2281 gaaatcggat gctgcaattc agagcggcag caagtggggg acagcagaag acctgaccgc
     2341 cgcagagtgg atgtttgaca tggtgaagac catcgcgcca tcagccagaa aaccgaattt
     2401 tgcagggtgg gctaacgata tccgcctgat gcgtgaacgt gacggacgta accaccgcga
     2461 catgtgtgta ctgttccgct gggcatgcca ggacaacttc tggtccggta acgtgctaag
     2521 tccggccaaa ctccgcgaca agtggaccca actcgaaatc aaccgtaaca agcaacaggc
     2581 tggcgtgaca gccggaaaat caaaactcga cctgacaaac actgactgga tttatggggt
     2641 ggatttatga aaaacatcgc cgcacagatg gttaactttg accgtgagca gatgcgtcgg
     2701 atcgccaata acatgccgga acagtacgac gaaaagccgc aggtacagct ggtagcgcag
     2761 atcatcaatg gtgtgttcag ccagttactg gcaactttcc cggcgagcct ggctaaccgg
     2821 gaccagaacg aactgaacga aatccgccgc cagtgggtgc tggctttccg ggaaaatggg
     2881 atcaccacaa tggaacaggt taacgcagga atgcgcgtag cccgtcggca gaatcgacca
     2941 ttcctgccat cacccgggca gtttgttgcc tggtgccggg aagaaacatc cgttaccgcc
     3001 gggctgccaa acgccagcga gctggttgat atggtttacg agtattgccg gaagcgtggc
     3061 ctgtatccgg acgcagagtc ttatccatgg aaatcaaacg cgcactactg gttggttacc
     3121 aacctgtacc agaacatgcg ggccaatgcg ttgactgacg cggaattacg gcgcaaggct
     3181 gccgatgaac tggcctgtat gaccgcgcga attaaccgtg gtgaggctat acctgaacca
     3241 gtaaaacaac ttcctgtcat gggcggtagg cctctaaatc gtgcacaggc tctggcgaag
     3301 atcgcagaaa tcaaagctaa gttcgggctg aaaggagcga tgaaatagag cta
//
LOCUS       DADSZD010000276         3284 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.278, whole genome shotgun sequence.
ACCESSION   DADSZD010000276 DADSZD010000000
VERSION     DADSZD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.278"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..815)
                     /locus_tag="J1Z28_004350"
     CDS             complement(<1..815)
                     /locus_tag="J1Z28_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7719107.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKISEHE
                     QSRRHPDASLTAKGFTQLSSATNSESEILAATPKAVKAVYDLAAGKAPVSHTHPWNQI
                     TGVPAASLTVKGTVQLSSATNSTSETQAATPKAVKAVYDLAAGKAPVSHTHPW"
     gene            complement(812..1417)
                     /locus_tag="J1Z28_004351"
     CDS             complement(812..1417)
                     /locus_tag="J1Z28_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HBA7719108.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGEAPGTFRLDIGVQDHGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(1410..2318)
                     /locus_tag="J1Z28_004352"
     CDS             complement(1410..2318)
                     /locus_tag="J1Z28_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBA7719109.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPATKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(2305..2664)
                     /locus_tag="J1Z28_004353"
     CDS             complement(2305..2664)
                     /locus_tag="J1Z28_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HBA7719110.1"
                     /translation="MTLYSGMNNTSGKAITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWESRLMLDSITINSNFDGSMVVGLTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(2661..3239)
                     /locus_tag="J1Z28_004354"
     CDS             complement(2661..3239)
                     /locus_tag="J1Z28_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HBA7719111.1"
                     /translation="MNAHLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGDLETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAVDNITLKTSEFVLEADRTRINSDVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
BASE COUNT          689 a          987 c          856 g          752 t
ORIGIN      
        1 ttccacgggt gtgtgtgact gacaggtgcc ttacctgctg caaggtcata tacagccttc
       61 actgcctttg gagtggcagc ctgcgtttct gatgtgctgt tagtggcgct gctgagttgc
      121 acggtgcctt ttaccgtcag cgaggctgca ggtacacctg ttatctgatt ccacgggtgc
      181 gtgtgactga caggtgcctt acctgctgca aggtcatata cagccttcac tgccttcggt
      241 gttgcggcca gtatttcgga ttcactgttg gtcgcactgc ttaactgagt aaaacctttt
      301 gcggtcagcg aggcgtccgg gtgacgtcgt gactgttcgt gctctgatat tttgtcatcc
      361 acatacttgc gagttgccag tactacagca gggtcgattt tcagggtgat attgtccgtg
      421 ctgctggtaa tcaacaccat acgcacggtc tgggtacgcc cgctgccttc agccagttgc
      481 ggcttatagc tttccgggca gtttcccacg gcaatcaatg ccccggactc atcaaacagg
      541 cccacttcac gtatccacca accgccctcg ttttcaggga tcacctgttc agcaataatc
      601 tggctgctgt tctgcgggtc gatataaagc atattcaacg cagcccggcg tttctcattt
      661 accagtgccg tctgctttgc gtccggcgtc ggtaatactc cgccgccatc gccgaccgcc
      721 atatgggtaa tttttagcgg cacaccgagc gcggcggcgc tggcaagttt cgccgcgcca
      781 atatccgtca gcagggtata aaattttgtg ctcatggatt cactctcatt gtgtcaataa
      841 catggaccgc cccgccttca tgcgcggtgc caccggaaat aatcgtttcg ttgatatacg
      901 gatagatcgt gatttcttcg ccaagatagc tggcggctcc cacccaatgc gggccgctgg
      961 tctgcagatt gatggacatg ccgatcatgt ggcggctaca tggtttggca tcgcttatca
     1021 gtcgctcaag ttccagatag gtatcttcag tgatgccatg gtcctgcacg ccgatatcca
     1081 ggcgaaacgt gcccggtgcc tctccggtct gccaccactc aataatgcgg atcagaaagc
     1141 cgaacggctc caccacccgc cgcacagcac tggtggtccc tttatgctga tgaatataaa
     1201 aagcatcctt caccacctgg cgcttgacgc tttctgtcca gccctcgtcc cagcgatcca
     1261 cagagaacgc ccaggcgaga taaggcagga aactgaccgg acaggttgcc ggattccaca
     1321 agtcacgaag cggcacctgc agatcagaaa tcccgctgca ggtttgcgcc agtcggcgct
     1381 ccagtggtgt tgaacccggt ggcagcagac tattcatccg ttcctccgtt ggttacgctc
     1441 cactgcgtac atgatgccgc ctgtgttttg ttcaggacca catccgccag aggagaagcc
     1501 agctccacac gctgcacacc ctcaacatgc agggcggcaa agatggcgct acggcgaata
     1561 tcccgaccaa gacgcgtctg actggcgatg tacttctgca ggctggcttt tgccgctgcc
     1621 atcaccggct ctgcttccgg tccaggatag agaaaaatgg tggcttccac gcgatacggg
     1681 atgatttctg cgctgcgaac cgtaagacgg tcagccaccg ggcggacgtt ctcactgttc
     1741 agagcttttt ccaccacgtc cagcaggtct ttttctgcag ttccatcgcc ttcgcggcta
     1801 agaacagtca gcaccacctc tgcaggtgcc gggctggttg cactggcatc cgccacccga
     1861 ccgtcggcgc ttcgggcatg aaactcataa gctgcagttg gccccgcaac agaaagccct
     1921 tcaaaggctg caggcacacg caggcgtaac gcttcatcgc tttccatcac agctgcaacg
     1981 ggcggcacag catcattatc agcaggcgtc accgtcaggc gtgtcacgtt gtagttggca
     2041 gcgagctggt caagatcgcc gcccatcgcg taagccacca tcaccgcctg cgcggcttcg
     2101 ttaatgcgct ggcgcagaag caactcacgg taagcgttct cctgcaacaa tttagtggcg
     2161 ggttcagatt ccagttccag cgtgcggatc acggcttcct gctcatcttt cggatgaagc
     2221 gccacaaatt ctgccttgcg ttcggcaagc agcgtctcaa agtccggcac atcgacaatc
     2281 tgcggcgcag gcaactgcga aaggtcaatc actgccattc tctgctcctg ttgatacgga
     2341 aagggaaaca ggcacaccgt tattacgccg cccggtcagc cccaccacca ttgaaccgtc
     2401 aaaattgctg ttgatggtga tggaatccag catcagccgt gactcccagc gactcagtgc
     2461 cacatacaca gccgacatga cctgcagccg taatgccgga ttttgtggct gatctattaa
     2521 agccgacagc agagaaccat attcacgacg agcaatgcgg ctaccctgcg gcgtcagcag
     2581 aatgtcccgc accgactggc gcagatggtc aatatcagta atggctttgc cgctggtatt
     2641 gttcatcccg ctataaagcg tcataccggg cctccggttg tgtcgccgcc tttcaggaca
     2701 ccagtatgct gatgcgcatc aaccacgatc ccgttagaac tcatcgctcc gccgccctgg
     2761 gtaacaccgc cattgatcac cacgtcgctg ttaatgcgtg tgcggtcagc ctccagtaca
     2821 aactcactgg ttttcagggt gatgttgtcg acagcctcaa tgaccattga tttgatgccc
     2881 ctgacatacc agcgcccggt ggcgggttcg tattcaaacc agccaccgtc aggatgttct
     2941 gtcacgcagg cgtccgccga cgtcgacggc ggtgcgaact gattcgaata gacagcgggc
     3001 aacgcaaagg cggtttccag attgccaccc agactcagca gcaccacctg ctcaccttcc
     3061 gatggtcgcc accatgtgcg ggcattcccg gcacgcagcg tcagccagct gatccagttg
     3121 gtttcaaggt cgcccgtttt cacccggcaa agccagttct cccggtccac ttcggtgact
     3181 acaccagtgc gaatcaggtt ggtgataagg cgcatgattt cggttaaatg tgcgttcatg
     3241 agaaaaaact aaccgccatt gcaaacgcaa tcgagtagat agga
//
LOCUS       DADSZD010000277         3245 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.279, whole genome shotgun sequence.
ACCESSION   DADSZD010000277 DADSZD010000000
VERSION     DADSZD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.279"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..326)
                     /locus_tag="J1Z28_004355"
     CDS             complement(<1..326)
                     /locus_tag="J1Z28_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719112.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMA"
     gene            complement(354..1145)
                     /locus_tag="J1Z28_004356"
     CDS             complement(354..1145)
                     /locus_tag="J1Z28_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001639317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBA7719113.1"
                     /translation="MRAPFSTENEACHQPKVGDDIFADNTIRNLESWGINTTYVEKVP
                     CTSSGVAPIFVNANSSNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETV
                     YHAIEFGKKNGIEVLLNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIR
                     LAARSLVDKGLNNIIVTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHY
                     YVQNGDVEAALKKAALFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            complement(1255..2468)
                     /locus_tag="J1Z28_004357"
     CDS             complement(join(1255..2174,2174..2468))
                     /locus_tag="J1Z28_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBA7719114.1"
                     /translation="MIFSPQHKTGDLMNKKTKRTLTPEFRLECAQLIVDKGYSYRQAS
                     ESMNVGSTTLESWVRQLRRERQGIAPSAIPITPDQQRIRELEKQVRRLEEQNTIFKKG
                     YCTLDVRLAERFTIVARLSGSHTVVSLCSALGIHRSSYRYWQKRRDTVNPVHVRLCSE
                     IRRVWNQSRGSAGARTLADMLTQNGVPMSRYRAGRLMKHLNLSSCQPGKHQYKNARQE
                     HTCLPNLLGRQFAVPEPDQVWCGDITYIWAGNCWCYLAVVMDLFARRVIGWSLSAHAD
                     TALISDALRMAYETRGQPHDVIFHSDQGSQYTGLKYQQILWRYRIKQSVSRRGNCWDN
                     SPMERFFRSLKTEWVPANGYVDKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENR
                     YHFYCKTVASIT"
     gene            2670..2927
                     /locus_tag="J1Z28_004358"
                     /pseudo
     CDS             2670..2927
                     /locus_tag="J1Z28_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          897 a          800 c          724 g          824 t
ORIGIN      
        1 gccatatgcg aggcagggaa aaaaagtgta caaccaccaa tatacagcgt caggccaatt
       61 aaaattgcca ccttataact ggtcttttta atcacaaggg atgctggtat tgcaattaaa
      121 aaataaccgc cataaaatgc gctctgcacc aatgctgaag caaagttact tagcgaaaat
      181 acacttttga attgagtgat taatatgtca tttaatgcag ctgcgcatcc ccatagcggg
      241 aataaacacg ataacaaaat aaactggaac aagggagtct tattcagata cccatcaggc
      301 atctgaatga tgtttttatc gttcatagtg ctacctttaa ctgtgcagga tgattattcg
      361 tttaaggtta aaaattcatt aaattgttca atactcggat aagatgattg cgtacctttc
      421 cctgtgacgc tgaaagcggc aaagagagcg gcttttttca aagcggcttc aacatcaccg
      481 ttttgaacat aataatggga aaagcaacca ataaatgcgt caccagcgcc actagtatca
      541 acagcattta ctttgaatgc aggaacatgg acttcctgat cgcgggtcat ccataatgcg
      601 cctttttcgc tcatggtaac aataatattg ttcagccctt tatcaactaa cgaacgtgcg
      661 gccaaacgaa tatgatcata agtatcaacc gacataccgg ttaatatttc cagttctgtt
      721 tcattcggga taaagaaatc acatttgcag gcataagaca tatctaactc acgcaatgcc
      781 ggagccggat ttaataacac ttcaatacca tttttcttac caaactcaat cgcgtggtaa
      841 actgtttcca gttgaacttc cagttgtaaa acgatcaatt tgcatttttt cagatcttct
      901 gccgctcgat cgatatcttc cggggaaaga aatttattcg cccccttaat tattaaaata
      961 ctattgctcg agttggcatt aacaaagatc ggtgcaacac cactgctggt acaggggacc
     1021 ttctcaacat aagtggtatt aattccccat gattcgagat tacgaatagt attatccgca
     1081 aaaatatcat cacctacttt cggctgatgg caagcctcat tctctgtgga aaaaggggcg
     1141 cgcattagct tctcaaccag agagcgtcac aaagacaaat attgctgaaa atcgcattca
     1201 tcgttcagac gtgaatgccc ggagacaaaa gccgctgtcc gtacagtgtt gtggtcaagt
     1261 aatactggcc acggttttac agtaaaaatg gtacctgttc tcggactctt ccggagtcaa
     1321 cccaccgtta taatggtgag gtctgacact gttgtagtag ttcaatatgt aatcattaat
     1381 ttgctgccgg gcctcgtcct tgtcgacgta accattcgct ggcacccatt ctgttttcag
     1441 actgcggaag aagcgttcca ttgggctatt atcccagcag tttccccgcc gactgacact
     1501 ttgctttatt ctgtaacgcc agagaatttg ttgatatttc aggcctgtat actggcttcc
     1561 ctggtcgcta tggaatataa catcatgtgg ttgaccacgc gtctcatagg ccatccgcag
     1621 ggcatcgctt atcagtgcag tatcggcatg tgcggacaga ctccagccga taaccctgcg
     1681 ggcaaaaaga tccatcacaa ccgccagata gcaccagcaa tttccggccc agatataggt
     1741 aatatctcca caccatacct ggtctggctc ggggacagcg aactggcgcc cgagcagatt
     1801 cggcaagcag gtatgctcct gacgggcatt tttgtactga tgttttccgg gctgacaact
     1861 gctcagattc agatgtttca tcagccgccc ggcacggtaa cggctcatcg ggacgccgtt
     1921 ttgggtcagc atgtcagcca gcgtgcgcgc ccccgcagag ccccggcttt ggttccacac
     1981 ccggcgtatt tcactgcaca acctgacatg caccggattc accgtatcgc gtcgtttttg
     2041 ccagtaccgg taactgctgc ggtgtattcc cagagcagaa cacagactga caacggtgtg
     2101 gctgccactg agtctggcaa ctatcgtgaa ccgttcagcg agtcggacat caagagtgca
     2161 gtagcctttt ttaatatcgt attttgttcc tccaggcggc gaacctgctt ttccagctcg
     2221 cggatacgtt gctggtctgg agtaatcggt atggcagagg gggcaatccc ctggcgctct
     2281 cgtctgagct gacgtaccca gctctcaaga gtggtagagc cgacattcat cgattcactg
     2341 gcttgtcgat atgagtagcc cttatcaaca atcagttgcg cacattccag tctgaactcg
     2401 ggggttaaag tacgtttggt tttcttgttc attaagtcac ctgttttgtg ttgaggtgag
     2461 aatatcacct ttaatcaggt ggccaaactt actgtgccac tacacagccc ccctctgacc
     2521 tgaacaaaaa gcatacacac aacaggctca cctgaccccg aaaaaccata acttcagcaa
     2581 taccagtgac accagcacgt tacctcagtg ccacatcttc agaccgcgat caatctgaaa
     2641 tgtgactatc ggggcaatat ccctgctttg tggtacacga taaccagaaa acatcccctg
     2701 aaccgccagc tagaaagact ggccacaaaa gggggaagct gaaatcgttt atggattaca
     2761 tcgacatatg cctggcagag aatgtccgga atgacgagat tatctttgcg gagattaagg
     2821 caatgggcta tacgggccga cgttccatgc cgcgttacta cattcagccc ggacgtaaaa
     2881 tgcgcccgtc aaaaagaacg gttcgcttcg aaactcagcc aggataccac ctctccgcca
     2941 cacccgggaa gaagttgata tggtggttgc cggacaacag cgctcctgga ttgactgctg
     3001 catcactcaa ccacgctgaa tatcaaagga gagcgttacc ggttaaaaga gaaacgtaaa
     3061 gctatggtga tgacccaaaa cgcaacgcct gtcagtgagg atgaaatggt gaaaagcgga
     3121 cagcatcagt aacggaagta tcttagcggg tacgaaaatg gcaggatcgt gacattgaca
     3181 gaatatctcc tgatcatcct gaatgactgc agttgctgtg gaaaagtaag ctgtcttcac
     3241 ccacg
//
LOCUS       DADSZD010000278         3240 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.280, whole genome shotgun sequence.
ACCESSION   DADSZD010000278 DADSZD010000000
VERSION     DADSZD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.280"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(3..329)
                     /locus_tag="J1Z28_004359"
     CDS             complement(3..329)
                     /locus_tag="J1Z28_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBA7719115.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWVRKF"
     gene            complement(337..522)
                     /locus_tag="J1Z28_004360"
     CDS             complement(337..522)
                     /locus_tag="J1Z28_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBA7719116.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(519..3158)
                     /locus_tag="J1Z28_004361"
     CDS             complement(519..3158)
                     /locus_tag="J1Z28_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBA7719117.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILEKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNAHLPGNDCPQGKECEDKQ"
BASE COUNT          791 a          899 c          733 g          817 t
ORIGIN      
        1 tattaaaatt ttcgcaccca tttaccgttt atcccttgta catattcccc gggtttagcc
       61 cgttctacga gcttttgccc ggcgagtttc gcgatatcat ccaccgacac attattttgc
      121 tttgccagtt gttgatagct cgctttacgt tcggcattga tgtcggttac taatttctca
      181 gtttcagcat ctgttttcag cgcaaccaga taaccataaa atgtttcccc tacccgcccc
      241 tgagttctgg cttcatccag agtcaatgcc attacattgc tgcttaccaa gccaacaaga
      301 aatgcacaaa gaagtaatgt tcttttcatc atcacctcaa aaaagatcgc tacgggtctc
      361 aagcagctct tcgacatctt tgtctgcctt gatgatgatc tcatgctcaa ttttaacgtt
      421 catattgata gtgatcggtt ccttaggtgc agcgacttca atgcgaggcg tacagccaac
      481 cagcataaaa gatgacgtca acgcagccag taaaattttc attgtttatc ctcacactct
      541 tttccttgcg gacagtcatt tcccggtaga tgcgcgttct gctccagcca tgcctggaga
      601 ttatcgccaa agcgtaaact gcgccacagc gtaaaaatat tctcttcatg atggtaatta
      661 agatttaccg taccgctttt accatcaacg cgactggtac ctgtaatatt ggcctgcatg
      721 gttaataaac cgagattatc taaattaatt tttgtcgatg aacggctaat ttccatatag
      781 cgcaaccagt taattgctga acccgcggtc atattgtctt tcaccaccgc atctgcggtg
      841 tctttatcca gccgcaaggt catcggcccg ctatttgcca gccagccgtc tttcactatc
      901 catttttcgt tgtttagcca taacggcaat gcaccactaa atgccccgga catggcgaat
      961 tgtttcggat tgacggcgct aaccagttcg ctggaggaaa gattattcag ccgcaacagc
     1021 gccgggtcat gttgcggcat acgtaattgt ttcatcagca cgttaccgcc taacacatcg
     1081 acgctaacat cagtcaacag caatggttct tcttcagtcc acggataacg accttgcaaa
     1141 tcagccgtaa tatttttcgc tgtcaccaga ttaatcactt cggcaattcg caacgtaacg
     1201 ggaccgcgag tccccagatg ccaggctcca tcggcaaaac ggaaaggcag gacaaaatcg
     1261 acaccgttaa cctgattatc tggcatccag gcactaccac ccttcaacac gccgtgccct
     1321 cccgcgcgga atccttgttc aggcgcagct gaaaatgcaa cctgagcata cagttcacca
     1381 tcgcgtaagt tcatcttcca gtcgggtggc accagcggct ggaatacagt cagtgattgt
     1441 ttaggccacc aggcgttacc gcgcagacga ataccgtccc agcgaccatt tacccgaacc
     1501 gggccaatct caccagcatg taaatcgcct ttaaagagga aatacgtcgg atcgcgccca
     1561 tcgacactaa attttaaggt tgatggcggt aacacactgc cgccagtgaa cagcgtttgc
     1621 ccggcgtcca gtgacagcgc gccgctgaat gagggatgct gtgcgtcacg tccccagacg
     1681 acgggcttct cgagaattaa tcgcggcttt tctaccgtca tcgtaccgta ttgtaactgg
     1741 tcgaaaccgg tggagagatc ggtcagcgta atcgtgctgt catgccactc accttttcct
     1801 gcgacatccc aggtggcatt catcggtgta aaactccctt ttccccagta gcgccactgc
     1861 cagcggccag cgtcagggag aaaatcattc gccagcccat ccatatgcag cacgaaatcg
     1921 cccagttcat tttcatgcgc ctgcaaaatg gcctgcaaac gtccgtcaac accacgttgg
     1981 gtgactttta cacccgctaa aggccagcgg atttcatcga tgtccagcga atcaatgact
     2041 cttccctttg aacgaagtaa cgcgccaggc tcaaaggtca gcgttgggtc agacagactt
     2101 ccacttagct gcgcaggtaa acgggcatat aaaatgaggt ccgcctgttt cgcttcaccg
     2161 gtcagctgca gaggcagctg gctgttatcc atgcttaatt ttcctgggcc aaaatttaac
     2221 accgcgttgc ctttacccgc ttgcccctgg gtcaacacac tcagtcgccc gctgaccaga
     2281 gcgttctcga gccctgcctg ccagttgtcg actttgacac ccagtcggcc actcaaagga
     2341 aaacctgcat acggccagct ccagcgacca tcgcttacgg tcaattgttg acgagtaatt
     2401 tgccacggca aatcgagcaa cggatcgccg ttatcccgtg ccagcacaat caattgcccg
     2461 ctattttcct gccagtccag ctcggcatcc accagtgacg gttcctgcgg caagtttaac
     2521 gtcgcagtag catgaccact taccggaagt ccatccggca cgagcggcat agtaaattcc
     2581 cccaccagtt ttaccggcgg ctgattttca aacgcggcga catccagttc gctgactgta
     2641 agttgttgcc ctttcagctg gccttgaaat ttaacttttt cgccctgata acgcagttgc
     2701 tggatatcgg aggttaatgc gagagagagt tttccctgcc attcctgcca gggagaaaaa
     2761 atcagtttat cgatattgat ccaggtgtta ggcagcatgg actgccactg cgcgagggtt
     2821 tttggagcgg ctggcgattg ctccgtctgc ggcaatttcg ccagacaagc agaatcaagt
     2881 tctaccgtgc cgacgttcaa taaccagcgg ctgggatgtg aaaggctggc gttggtgata
     2941 tgcgcaagct gacaatctcc caccagataa cggagatcgg ggatgattaa acctttacgc
     3001 gtaatgcgtg ggctttcatc taatgcaata cgtgtcccga gcggtagcca gatgcccgcc
     3061 agcgtgggaa cccacagccc gagcgtcatc agcagcgtca acggcacaag aataatcagt
     3121 aataacagcg cgagaacggc tttatattta cccagcatgg gtagttaata tcctgattta
     3181 gcgaaaaatt aagcattcaa tacgggtatt gtggcatgtt taacccttca gttgaaggtt
//
LOCUS       DADSZD010000279         3228 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.281, whole genome shotgun sequence.
ACCESSION   DADSZD010000279 DADSZD010000000
VERSION     DADSZD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.281"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(93..620)
                     /gene="mobB"
                     /locus_tag="J1Z28_004362"
     CDS             complement(93..620)
                     /gene="mobB"
                     /locus_tag="J1Z28_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBA7719118.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(602..1186)
                     /gene="mobA"
                     /locus_tag="J1Z28_004363"
     CDS             complement(602..1186)
                     /gene="mobA"
                     /locus_tag="J1Z28_004363"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBA7719119.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1256..1525
                     /locus_tag="J1Z28_004364"
     CDS             1256..1525
                     /locus_tag="J1Z28_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBA7719120.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1602..2588
                     /gene="srkA"
                     /locus_tag="J1Z28_004365"
     CDS             1602..2588
                     /gene="srkA"
                     /locus_tag="J1Z28_004365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBA7719121.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTVTFIEQAKVLQEPPLQLTPMY"
     gene            2605..>3228
                     /gene="dsbA"
                     /locus_tag="J1Z28_004366"
     CDS             2605..>3228
                     /gene="dsbA"
                     /locus_tag="J1Z28_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBA7719122.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          816 a          784 c          792 g          836 t
ORIGIN      
        1 gcgaaaggcc atccgaaagg atggcctttt tgcattggag cagaataaat gccggatgcg
       61 acgctggcgc gtttgctcca gcctactctt gcctatccgt cctgcttttg catccactcc
      121 accacaaaat cagccagccc ctcaacatca ttaatatcca gtaacgcgac atcaagatta
      181 agcggcacat cactggctac agcaataaca tgcctgtcta tcactaattc ttccggtcga
      241 tgtccggctc catcgcgaaa cagcacaatc tttgcgatct cttcatgctt aaacccttcg
      301 accagaatca aatccagctt tgaggtatcc attcgacttg cgagaaaatg tagatccagc
      361 tcttcttcgt ctggtgtttc cgtcatcaag gcccatcgct gttggctggc aacgatggtt
      421 tgcgccgcgc cagccttgcg cagctcatag ctatctttgc ccggtttatc aacatccata
      481 tcatggtgcg tatgcttaat cagccccgga cggatccctc tggcgcataa tgccgggatc
      541 aattttttca gcagcgttgt ttttccggtg ccactccatg cagcaaaggc gagtaacggt
      601 atcatcgttt ttcctgccat cgggcaagct cctctggcgt attcacgttt ataaatgcct
      661 ctttacgatc gctgaaatca accgcatgac cgccagccag acgcataaat gccataaccc
      721 ggcgttctcc tgcttgcaga tattccagta ataaaggctc aattgcgcgg tttaccagag
      781 caatagtcgg gtgatcgcgt tcaccgtcgt ggacccacac gacaggcgca tctttgcgct
      841 gatgagtaag ccgggcggct aaatcatggg gaatgtaagg cgtatcgcac gggcaaaaca
      901 aaaaccactc acccgcttcc tgctgcatta ctgaaagcat tcctgccaga ggaccagggt
      961 aatccgccag tgaatcttca atcactttca gaccgcttac ctgatagatt tcctgatgac
     1021 gattagcatt aaccacgacg tgagaaagct gcgtcataag cgcgtcagcg acatgctgcc
     1081 ataatggttt gccgtttaat tcaagcaatc ctttatctac gccgcccatt cgtctggctt
     1141 taccgcccgc cagcacaacg cctgttatcg tcgtcatcag attcaccgat atcgcctctt
     1201 ttattgtggg attgaccctg ctaacgtgtc tgtctcaaga gtaaggagca ttactatgaa
     1261 atgtaaacgt ctgaatgaag ttattgaact cctccagcca gcctggcaaa aagagccaga
     1321 ccttaacctg ctgcaatttt tgcagaaact ggcgaaagag tcaggttttg acggcgaact
     1381 ggcggatttg acggatgaca ttctgatcta tcacctgaaa atgcgtgatt ccgcgaaaga
     1441 tgcggtgatc ccgggtttgc agaaagatta tgaagaagat ttcaaaacgg cgctattacg
     1501 cgcacgtggc gtaattaaag agtaaaagct tgtaagcggc gcaaccaaaa tcatcgtgaa
     1561 atgatatcct tcatcattcg taatgttttc cggatgatgg gatgaacaac agcgctttta
     1621 ctttccagac actacacccg gataccatca tggacgctct gtttgagcat gggattcggg
     1681 tggattccgg tcttaccccg cttaacagct atgaaaaccg tgtctatcaa tttcaggacg
     1741 aagatcgtcg acgttttgtc gtcaaatttt atcgccctga acgttggaca gccgatcaaa
     1801 tcctcgaaga acatcaattt gcgttgcagc tggttaacga tgaagttccg gtcgcagcac
     1861 ctgtggcctt taatggtcag actttattga accatcaggg attttatttc gctgtttttc
     1921 caagcgtcgg tggtcgccag ttcgaagctg ataatatcga tcagatggaa gcggttgggc
     1981 gttatttagg gcgtatgcac cagacggggc gcaaacagct ttttatccat cgcccgacca
     2041 tcggtttgaa tgaatatctc attgagccac gcaagctgtt tgaggacgct acactgatac
     2101 cttccgggtt gaaagcggca ttcctgaaag cgacagatga gctgattgcc gccgttacag
     2161 cacactggcg ggaagatttc accgttctgc ggctacatgg agactgccac gccgggaata
     2221 ttctctggcg cgatggtcca atgtttgttg atctggatga tgcacgtaat ggtccagccg
     2281 ttcaggattt gtggatgttg ctcaatggtg ataaagccga gcagcggatg caactggaaa
     2341 ctattattga ggcttatgaa gaatttagcg agttcgacac cgctgaaatc ggactgattg
     2401 aacctttacg cgccatgcgt ttggtttatt atcttgcctg gctaatgcgg cgttgggctg
     2461 atcccgcgtt cccgaaaaat ttcccgtggt taaccgggga agattactgg ctacgacaga
     2521 cggtgacttt tatagaacag gcaaaagttc tacaagaacc ccctttgcaa ttaacaccta
     2581 tgtattaatc ggagagagta gatcatgaaa aagatttggc tggcgctggc tggtttagtt
     2641 ttagcgttta gcgcatcggc ggcgcagtat gaagatggta aacagtacac taccctggaa
     2701 aaaccagttg ctggcgcgcc gcaagtgctg gagtttttct ctttcttctg cccgcactgc
     2761 tatcagtttg aagaagttct gcatatttct gataacgtga agaaaaaact gccggaaggc
     2821 gtgaagatga ctaaatacca cgtcaacttc atggggggtg acctgggcaa agagctgact
     2881 caggcatggg ctgtggcgat ggcgctgggc gtggaagaca aagtcacagt tccgctgttt
     2941 gaaggcgtac aaaaaaccca gaccattcgt tcagcatctg atatccgcga tgtatttatc
     3001 aacgcaggta ttaaaggtga agagtacgac gcggcgtgga acagcttcgt ggtgaaatct
     3061 ctggtcgctc agcaggaaaa agctgcagct gacgtgcaat tgcgtggtgt tccggcgatg
     3121 tttgttaacg gtaaatatca gctgaatccg cagggtatgg ataccagcaa tatggatgtt
     3181 tttgttcagc agtatgccga tactgtgaaa tatctgtccg agaaaaaa
//
LOCUS       DADSZD010000280         3226 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.282, whole genome shotgun sequence.
ACCESSION   DADSZD010000280 DADSZD010000000
VERSION     DADSZD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.282"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            42..533
                     /locus_tag="J1Z28_004367"
     CDS             42..533
                     /locus_tag="J1Z28_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBA7719123.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEGIQTDPFQTRYEKEGLLTTDLTALAEGSTPSITGETSTSLAALLKPIKKPL
                     KGN"
     gene            536..1804
                     /locus_tag="J1Z28_004368"
     CDS             536..1804
                     /locus_tag="J1Z28_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HBA7719124.1"
                     /translation="MALIGVYADWEGLDGPERIGYLHSRRTRTREIFEFEYDKKALAD
                     PSLNFIQLDPEIMLYEGAQYPIPPKDKFGAFSDSCPDRWGRMLMKRRFERDIRDGLCD
                     KDSHLYESDYLLGVHDLYRVGALRYKREDAGEFLDNRIDVAAPPFTEIASLERASRAI
                     EEDPDNKELMGQKWLRMLIAPGGSLGGARPKASVVDEAGHLYIAKFPSVKDEYDVGGW
                     EMVVNALAVGCGLNVAPAQAHKFASNYHCFMVRRFDRTNAGRRLHFASAMTLTRHQDG
                     EDASTGVSYLELADVLIRHGAQTNTDLKELWSRIVFNILVSNTDDHLRNHGYILIPGK
                     GWRLSEAYDLNPVARSDGLKLNITENDNALDLELAREVAEYFRLGLTEADDIIANFRG
                     IVSQWRIIAERLRLPGREQELMAEAFRGAI"
     gene            complement(1947..3224)
                     /locus_tag="J1Z28_004369"
     CDS             complement(1947..3224)
                     /locus_tag="J1Z28_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7719125.1"
                     /translation="MNKIKNYRWHMIALVCFITVINYLDRTALGIAAPTIMETTGITK
                     EQYSWIVSAFQLAYTLGQPIMGFFIDTMGLKLSFAICAAIWGLATMGHALTGTWSGLA
                     FMRALMGFSEASAIPAGVKTASTWFPAKERGVATGVFNMGTSLGAMLAPPLIAWCIMF
                     HSWQFAFIVSGSLALLAALFWFFCYKDPKDAKRLSDEERHYIESGQEQHLKTDKKEKT
                     SIKHILSQRNFWGIGIARFLADPAWGTINFWVPIFFVETLHFSLKEIAMFVWLPFLLG
                     DLGCLASGFVAKFFHDRGVSLINSRRITFTIAAVIMMTIGLVSIVENPYIAVLLISIG
                     AFSHQCLSTVAATLGGDLFKKDEVATAVGMAGACAWSGQLIFNLFIGAFVHIIGFAPF
                     FIALAFFDIIGAIALWTLIKVKDEEPQVQLATS"
BASE COUNT          929 a          659 c          775 g          863 t
ORIGIN      
        1 ttatggctat catagccata aaaagataaa atattatcac tatgggtaaa aaaacagcac
       61 cgttactccc ttatacgaat cagatgctta gtgaatttgg tgagcggctg aaacttgctc
      121 gtttacgacg caggcttacc gcaaagcaag ttgctgaacg tgctgggatg tcgccaataa
      181 cattgcgctc tctggagtca ggaagtgcag gggtcacgat aggggcatat ttgtctgtga
      241 tgcaggtttt gggcctggaa acggatttaa ataagcttgc agctgaagac gacttagggc
      301 gtcagctcca ggattcccgt ttgattaaaa aaagagggct ttctcgttca tcaggtaaaa
      361 atgaagaagg tattcagaca gacccttttc aaacgaggta tgagaaagaa ggtttactca
      421 ccacggattt aacagcactg gcagaaggct caacgccatc tattacagga gaaacatcaa
      481 cctctcttgc agcactatta aaaccgataa aaaaaccgtt aaaaggaaat taattatggc
      541 attaatcgga gtgtatgcgg attgggaagg cctggatggt ccggagcgga ttggttattt
      601 acacagtcgc cgcactcgga cgcgtgagat atttgagttt gagtacgata aaaaggcgct
      661 tgcagatcct tcacttaatt tcattcagtt agatcctgaa ataatgttgt atgagggagc
      721 acagtaccca attcctccaa aggataaatt tggtgcattt agtgattcct gcccggatcg
      781 ttgggggcgt atgctaatga agcgtcgttt tgagagggat attcgagacg ggttatgtga
      841 taaagactcc cacctttatg agtctgatta tctactaggt gttcatgatc tttatcgggt
      901 tggggctctt cgatacaaac gtgaagacgc tggagagttt cttgataatc gcattgatgt
      961 tgcagcccca ccatttaccg aaatagcgag tctggaaagg gctagccggg caattgaaga
     1021 agatcctgat aataaagagc taatggggca aaaatggtta agaatgctaa tcgctccggg
     1081 gggatcatta gggggggcac gtccgaaagc cagtgttgtt gatgaagctg ggcatctcta
     1141 catcgctaaa tttcccagtg taaaagatga gtatgatgtt ggtggatggg aaatggtcgt
     1201 taatgcactt gctgttggct gtggactgaa tgttgcacct gctcaggcgc ataaatttgc
     1261 cagcaattac cattgtttta tggttcgtcg ttttgaccgc actaatgcgg gaaggcgtct
     1321 gcattttgct tcggctatga cactaaccag gcatcaggat ggtgaagatg cctctacagg
     1381 tgtgagttat ctggaattag cagatgttct tatcaggcat ggcgcgcaga ctaatactga
     1441 tcttaaggaa ctctggtcca ggattgtttt taatattctt gtttctaata ccgatgacca
     1501 tttacgtaat catggatata tcttgatacc gggaaaaggt tggcggcttt ctgaggcata
     1561 tgatttgaat cctgttgcca ggtctgacgg tctaaaactc aatattacag aaaatgataa
     1621 tgcacttgat ttggaattag ctcgagaagt tgcggagtat tttcgacttg gtttgacaga
     1681 agcagatgac attattgcta attttagagg gattgtcagt cagtggcgaa tcattgccga
     1741 gcgtcttcga ttacctggtc gtgagcaaga gttaatggca gaggcgttca ggggggcaat
     1801 ttagaatagt tatttcgata tattcgctaa atcataaatt tgcgaagcaa tgccagtaga
     1861 tactgaacgt tttggaagag acgtttaatt tatgagaaaa atagaaacgc tacaaagcta
     1921 aattatagcg tttctatgta aaataattag cttgttgcta actgtacttg tggttcttcg
     1981 tctttaactt tgataagcgt ccacagcgca atggcgccaa taatgtcaaa gaaagccagg
     2041 gcaataaaga acggcgcgaa gccaataatg tgaacgaatg ccccgataaa caggttgaaa
     2101 atcaactgac cgctccatgc acaggctcct gccataccca cagcggtagc tacttcgtct
     2161 tttttgaata ggtcaccgcc cagagttgct gcaacagtag aaagacattg atgcgagaat
     2221 gcgccaatgc taatcagtaa tacggcgatg tagggatttt cgacaatact caccagacca
     2281 atcgtcatca taatgacggc tgcaatagtg aaggtaattc ttcgtgagtt aattaaactc
     2341 acgccgcgat cgtggaagaa cttcgcgaca aaaccactgg ctaaacagcc gagatcgccc
     2401 agcaggaaag gcagccagac gaacatggca atttctttca ggctaaaatg cagcgtttcg
     2461 acgaagaaaa tcggtaccca gaagttaatg gttccccatg ccgggtcagc gagaaaacgc
     2521 gcgatgccaa tcccccagaa attacgttgg ctgaggatat gcttgattga cgttttttct
     2581 ttcttatctg ttttaagatg ctgttcttgc ccagactcaa tatagtggcg ctcttcatca
     2641 gaaaggcgtt tagcatcttt tggatcttta taacaaaaga accaaaataa agccgcgagt
     2701 aaagcaaggc tacctgagac aataaacgca aattgccagc tatgaaacat aatgcaccag
     2761 gcaatcaacg gtggagcaag catcgcgccg agtgaggtgc ccatattgaa aacacctgtc
     2821 gccacgccac gctcttttgc cgggaaccat gttgatgcgg tttttacacc cgcaggaatt
     2881 gccgatgctt cgctgaaacc catcagggcg cgcataaatg ccagacctga ccatgttcct
     2941 gtcaaggcat gccccattgt cgccaggccc cagattgcgg cacatatcgc aaaacttaac
     3001 ttcagaccca tggtatcaat aaagaagccc attatcggtt gccccaatgt ataggccaac
     3061 tggaatgcac tgacaatcca tgaatattgt tctttggtta ttccggttgt ctccataatc
     3121 gttggagccg caatacctaa cgccgttctg tccagataat tgattacagt gataaagcat
     3181 acgagggcaa tcatatgcca tcgataattc tttattttat tcattt
//
LOCUS       DADSZD010000281         3195 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.283, whole genome shotgun sequence.
ACCESSION   DADSZD010000281 DADSZD010000000
VERSION     DADSZD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.283"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            130..1023
                     /locus_tag="J1Z28_004370"
     CDS             130..1023
                     /locus_tag="J1Z28_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7719126.1"
                     /translation="MLKENFNELQIFLVVARERSFTKAAGKLGVSQSALSHAMKALEE
                     RLNIRLLTRTTRSVAPTEAGERIIACLEPRIDELEQELESLIQLNGTPSGNIRLSAGE
                     HAARSLIWPKLKPFLREYPEINVELVVDNGFVDIVEGRFDAGIRLGENVDKDMVAVRI
                     GLDMRMAVVGAPAYFAANPAPETPHELQNHRCINMRLPTAGGLYHWEFEREGKPLRVK
                     VDGQLTCSPLPERIDAALSGFGIACVPEDMVQEYIKSGKLIQVLQEWCPTFPGYYLYY
                     PSRKQHPPAFALLIDALRYTE"
     gene            complement(1054..2043)
                     /locus_tag="J1Z28_004371"
     CDS             complement(1054..2043)
                     /locus_tag="J1Z28_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA7719127.1"
                     /translation="MQKRYLGKSGLEVSALGLGCMGLSHGYGPATDTRQAIELIRAAV
                     ERGVTFFDTAEVYGPYLNEEVVGEALKPFRDRVVIATKFGFTFGDDNKQQILNSRPEH
                     IREAVEGSLRRLKTDVIDLLYQHRVDPDVPIEDVAGTVKDLIAEGKVKHFGLSEAGAQ
                     TIRRAHAVQPVTALQSEYSMWWREPEQEILPLLEELGIGFVPFSPLGKGFLTGAIKPG
                     TTFGKDDYRSTVPRFAAQAIEANEKLVTLLGELAAEKGVTSAQIALAWLLAQKPWIVP
                     IPGTTKLHRLEENLAAADIVLSQKDTQQISEALETIKIVGERYSPEHQARVGR"
     gene            complement(2070..2921)
                     /locus_tag="J1Z28_004372"
     CDS             complement(2070..2921)
                     /locus_tag="J1Z28_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001195265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBA7719128.1"
                     /translation="MQKVKLNNGIEMPLLGFGVFQMTDAAECERAVIDAINSGYRLID
                     TAASYQNEIQVGNALKQSGIARNELFVTTKLWLQDTSYEGAKAQFERSLNRLQLDYVD
                     LYLIHQPYGDVHGAWRAMEELQQAGKIRAIGVSNFHPDRLADLIAFNNVVPAVNQVEV
                     NPFNQQLQAVPWMQSRGIQPEAWAPFAEGKNGLFQHPVLTAIGEKYGKSVGQVVLRWI
                     YQRGIVSLAKSVRKERMEENINILDFELSPEDMLQITALDTATSAFFSHRDPAMVEWL
                     TGRKLDV"
BASE COUNT          783 a          761 c          803 g          848 t
ORIGIN      
        1 ctgaaagttt agaattattt tcgatacagc cagattatca ttgttactaa taatgtggtt
       61 aaaataatag catctatgcc ccccttcatg agtgtctgtg aagaggatgc ttctacaagg
      121 aaatcgccaa tgctcaaaga aaacttcaac gaactgcaaa tctttcttgt ggtggcaaga
      181 gagcgaagtt ttaccaaagc agcgggcaaa ctcggcgtct ctcagtctgc acttagccat
      241 gcaatgaagg ctctggagga aaggctcaat atccgcctcc tgacacggac tacccgcagt
      301 gtggctccca cagaagcggg ggagagaatc attgcctgcc ttgaaccgcg tattgacgag
      361 cttgaacagg aactggaatc acttattcaa ttgaacggca cgccctccgg gaatattcgg
      421 ttatctgccg gggaacatgc cgcgcgaagt ctgatctggc cgaagctaaa accttttctc
      481 agagagtatc ccgaaatcaa tgtcgaactg gtggtggata acggttttgt cgatattgtt
      541 gaaggccgtt ttgatgccgg gatacgactg ggtgaaaatg ttgataaaga tatggttgca
      601 gtcaggattg gactagatat gcggatggct gtggtgggag ctccggcgta ttttgctgca
      661 aaccctgcgc ctgaaacgcc tcatgagcta caaaatcatc gctgcatcaa catgcgtttg
      721 ccaactgccg gtgggcttta ccactgggag ttcgagcgag aagggaaacc gcttcgcgtt
      781 aaggttgatg ggcaactgac gtgtagtcca ttgccagaaa gaattgatgc cgcgttatca
      841 gggtttggga tcgcttgtgt ccctgaagac atggttcagg agtatataaa gtcaggcaag
      901 cttattcagg tattacagga gtggtgtcca acttttcctg ggtattatct ttactacccc
      961 agccgcaagc agcatccgcc agcttttgcg ctgttgatcg atgcgcttcg ctacaccgaa
     1021 taacgggtcc tttatcctgg acccgtttgg gtattagcgg cctacgcgtg cctgatgttc
     1081 aggcgagtaa cgttcaccga cgattttaat tgtttccagc gcctcgctta tctgctgcgt
     1141 atctttctgc gaaagaacaa tgtcagcagc ggccaggttt tcttccagcc ggtgcagttt
     1201 agtggtgcca gggatgggaa caatccacgg cttttgtgct aatagccatg ccagtgcgat
     1261 ttgtgcagac gttacccctt tctccgcagc cagttcacct aacaaggtga ccagtttttc
     1321 attggcttca atggcttgtg ctgcgaaacg cggtacggtg ctgcggtaat catctttgcc
     1381 gaaagtggtt cccggcttaa tcgcccctgt caggaaacct ttacctagtg ggctgaaggg
     1441 gacaaaacca attcccagtt cctcaagcaa cggcagaatt tcctgttcag gctcacgcca
     1501 ccacatggaa tattcgcttt gcagggcggt gacaggttgt accgcatgtg cacgacgaat
     1561 ggtttgtgct cctgcttcag acaggccgaa atgtttgacc ttaccttctg cgatgaggtc
     1621 tttcaccgtt cctgcaacat cttcaatagg aacatctgga tcaacacggt gttgatacag
     1681 caggtcaata acatctgttt tcagacgacg taatgatcct tcaacggctt cacggatatg
     1741 ttccggacgg ctgtttaaaa tctgctgctt attatcatcg ccgaaggtaa aaccgaattt
     1801 agtggcaatt accacgcggt cacgaaacgg ttttaaggct tcgccgacca cttcttcatt
     1861 cagatacgga ccatacactt cggcggtatc gaagaaggtg acgccacgtt caaccgcagc
     1921 acgaataagt tcgatagcct gacgggtatc ggtcgccggg ccgtagccgt ggcttaagcc
     1981 catgcaaccg agcccaaggg cggagacttc gagtccggat ttacccagat aacgtttttg
     2041 cattgaatga atacctcttt tatcgcggct taaacatcaa gtttgcggcc agtcagccat
     2101 tccaccattg cgggatcgcg gtgagagaag aacgcgctgg ttgcggtatc cagggcggta
     2161 atttgcagca tatcttcagg gctgagttca aaatcgagaa tgttgatgtt ctcttccatg
     2221 cgttcttttc gcactgattt tgccagtgaa acaatgcctc gttggtagat ccagcgcaga
     2281 acaacctgtc ccacgctttt gccatacttc tcgccaattg ccgtgagaac aggatgctgg
     2341 aacagaccat ttttcccttc cgcaaacggt gcccaggctt ctggctgaat gccacgactt
     2401 tgcatccagg gaacggcctg tagctgctga ttgaaagggt taacttcaac ctggtttacc
     2461 gccggaacga cgttattaaa agcgataagg tctgccagtc ggtcaggatg gaagttactg
     2521 acgccaatag cgcgaatttt accggcctgt tgcagctctt ccatggcacg ccaggctccg
     2581 tggacatcgc catatggctg gtgaatcaag tacaggtcaa cgtaatcaag ttgcagtcga
     2641 ttcagagagc gttcgaactg ggctttggcc ccttcgtaac tggtatcctg caaccagagt
     2701 ttcgtggtga caaagagttc gttacgggcg ataccgcttt gtttcagtgc gttcccgacc
     2761 tggatttcat tttgataaga ggcggcggtg tcaatcagtc gataaccgct gttaatagcg
     2821 tcaatgaccg ctctttcgca ttcggcggca tcggtcatct gaaatacacc aaagcccaac
     2881 aggggcattt caataccgtt gttcagtttt actttttgca tggtgttatc cttccgttat
     2941 cgttcatgag aagcataacg taaagggaag agctcgatta gacggcagga tttgtcaggg
     3001 gttatgaaca aaattcataa atctgtttga gggttgagat tagtcgtgca agttcgatat
     3061 ccccggaatt tacagtccga tgaataaaaa tgaatgtcca taaaaaatat attggtttat
     3121 cccagcgtat atacttactt cgacgtactc ccaatagcag ttaattaaag acgggtcatt
     3181 attattcact ctgaa
//
LOCUS       DADSZD010000282         3161 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.284, whole genome shotgun sequence.
ACCESSION   DADSZD010000282 DADSZD010000000
VERSION     DADSZD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.284"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            150..1751
                     /gene="aceB"
                     /locus_tag="J1Z28_004373"
     CDS             150..1751
                     /gene="aceB"
                     /locus_tag="J1Z28_004373"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HBA7719129.1"
                     /translation="MTEQATTIDELAFTRPHGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGMLPDFISETASIRDADWKIRGIPSDLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEECFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            1781..3085
                     /gene="aceA"
                     /locus_tag="J1Z28_004374"
     CDS             1781..3085
                     /gene="aceA"
                     /locus_tag="J1Z28_004374"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBA7719130.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
BASE COUNT          796 a          790 c          863 g          712 t
ORIGIN      
        1 ataagttaac atgcattttt aattaaaatg gaaattgttt ttgattttgc attttaaatg
       61 agtagtctta gttgtgctga acgaaaagcg cacaacgatc cttcgttcac agtggggaag
      121 ttttcggatc catgacgagg agctgcacga tgactgaaca ggcaacaaca atcgatgaac
      181 tggctttcac aaggccgcat ggcgagcagg agaagcaaat tcttactgcc gaagcggtag
      241 aatttctgac tgagctggtg acgcatttta cgccacaacg caataaactt ctggcagcgc
      301 gcattcagca gcagcaggat atcgataacg gaatgttgcc tgattttatt tcggaaacag
      361 cttccattcg tgatgctgac tggaaaattc gcgggattcc ttcggactta gaagatcgtc
      421 gcgtagagat taccggacct gtagagcgca agatggtgat caacgcgctc aacgccaatg
      481 tgaaagtctt tatggccgat ttcgaagatt cactggcacc agactggaac aaagtgatcg
      541 atgggcaaat taacctgcgc gatgcggtta acggcaccat cagctatacc aatgaagcag
      601 gcaaaattta tcagctcaag cccaatccag cggttttgat ttgtcgggta cgcggtctgc
      661 acttgccgga aaaacatgtc acctggcgtg gtgaggcaat ccccggtagc ctgtttgatt
      721 ttgcgctcta tttcttccac aactatcagg cactgttggc aaagggcagc ggtccctatt
      781 tctatctgcc gaaaacccag tcctggcagg aagcggcctg gtggagcgaa gtcttcagct
      841 atgcagaaga tcgctttaat ctgccgcgcg gcaccatcaa ggcgacgttg ctgattgaaa
      901 cgctgcccgc cgtgttccag atggatgaaa tccttcacgc gctgcgtgac catattgttg
      961 gtctgaactg cggtcgttgg gattacatct tcagttatat caaaacattg aaaaactatc
     1021 ccgatcgcgt cctgccagac agacaggcag tgacgatgga taaacccttc ctgaatgctt
     1081 actcacgcct gttgattaaa acctgccata aacgcggtgc ttttgcgatg ggcggcatgg
     1141 cggcgtttat tccgagcaaa gatgaagagc gcaataacca ggtactcaac aaagtaaaag
     1201 cggataaatc gctggaagcc aataacggtc acgatggcac atggatcgct cacccgggtc
     1261 ttgctgatac ggcaatggcg gtattcaacg atattctcgg ctcccgcaaa aaccagcttg
     1321 aggtgatgcg cgaacaagac gcgccgatta ctgccgatca gctgctggca ccttgtgatg
     1381 gtgaacgcac cgaagaaggt atgcgcgcca acattcgcgt ggctgtgcag tacatcgaag
     1441 cgtggatctc tggcaatggc tgtgtgccga tttatggcct gatggaagat gcggcgacgg
     1501 ctgaaatttc ccgtacctca atctggcagt ggatccatca tcaaaaaacg ttgagcaatg
     1561 gcaaaccggt gactaaagcc ttgttccgcc agatgctggg cgaagagatg aaagtcattg
     1621 ccagcgaact gggcgaagaa tgtttctccc aggggcgttt tgacgatgcc gcacgcttga
     1681 tggaacagat caccacttcc gatgagttaa ttgatttcct gaccctgcca ggctaccgcc
     1741 tgttagcgta aaccaccaca taactatgga gcatctgcac atgaaaaccc gtacacaaca
     1801 aattgaagaa ttacagaaag agtggactca accgcgttgg gaaggcatta ctcgcccgta
     1861 cagcgcggaa gatgtggtga aattacgcgg ttcagtcaat ccggaatgca ccctggcgca
     1921 actgggtgcg gcgaaaatgt ggcgtctgct gcacggcgag tcgaaaaaag gctacatcaa
     1981 cagcctcggt gcactgactg gcggtcaggc gctgcaacag gcgaaagcgg gtattgaagc
     2041 agtctatctg tcgggatggc aggtagcggc ggacgctaac ctggcggcca gcatgtatcc
     2101 ggatcagtcg ctctatccgg caaactcggt gccagcggtg gtggagcgga tcaacaatac
     2161 tttccgccgt gcggatcaga tccaatggtc cgcgggcatt gagccgggcg atccgcgcta
     2221 tgtcgattac ttcctgccga tcgtcgccga tgcggaggcc ggttttggcg gtgtactgaa
     2281 tgcctttgaa ctgatgaaag cgatgattga agccggtgca gcggcagttc acttcgaaga
     2341 tcagctggca tcagtgaaga aatgtggtca catgggcggc aaagttttgg tgccaactca
     2401 ggaagctatt cagaaactgg tcgcggcgcg tctggcagct gatgtgacgg gcgttccaac
     2461 cctgctggtt gctcgtaccg atgccgatgc ggcggatctg attacctccg attgcgaccc
     2521 atatgacagc gaatttatta ctggcgagcg taccagtgaa ggcttcttcc gtactcatgc
     2581 gggtattgag caagcgatca gccgtggcct ggcgtatgcg ccatatgctg acctggtatg
     2641 gtgtgaaacc tccacgccgg acctggaact ggcgcgtcgc tttgcacaag ccatccacgc
     2701 gaaatatccg ggcaaactgc tggcttataa ctgctcgccg tcgttcaact ggcagaaaaa
     2761 cctcgacgac aaaactattg ccagcttcca gcagcagctg tcggatatgg gctacaagtt
     2821 ccagttcatc accctggcag gtatccacag catgtggttc aacatgtttg acctggcaaa
     2881 cgcctatgcc cagggcgagg gtatgaagca ctatgttgag aaagtgcagc agccggaatt
     2941 tgccgccgcg aaagatggct ataccttcgt atctcaccaa caggaagtgg gtacaggtta
     3001 cttcgataaa gtgacgacca ttattcaagg tggcacgtct tcagttaccg cgctgaccgg
     3061 ctccaccgaa gaatcgcagt tctaagcaac cgataaacgt tgctgactgt aggccggata
     3121 aggcgttcac gccgcatccg gcaatcagcg cctgatgcga c
//
LOCUS       DADSZD010000283         3138 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.285, whole genome shotgun sequence.
ACCESSION   DADSZD010000283 DADSZD010000000
VERSION     DADSZD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.285"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(940..1169)
                     /locus_tag="J1Z28_004375"
                     /pseudo
     CDS             complement(940..1169)
                     /locus_tag="J1Z28_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1750..2853
                     /locus_tag="J1Z28_004376"
     CDS             1750..2853
                     /locus_tag="J1Z28_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBA7719131.1"
                     /translation="MFPVYHFLVSAAILVFVVIFWRTHHRDHRNWLALRLFVLCSVNR
                     WPLRMVKGTVVGTTDTLREMQRYEQLSQHGADNWKIQPGAPLYDTIVIVTGESVRRDY
                     MSVYGYPVPTTPWLNTAPGLFIDDYTSTASSTVSSLSRTLIYDYEQNPDSGNNVVALA
                     AKAGYSTWWISNQGKLGKHDTRISVIASDAEHATFLKKGSFASRKTDDKLLLQETERA
                     LADTSSPKIIFLHMMGSHPNPCDSLNSWPNNYLEQYPRKIACYLASISKLDNFLGQLD
                     GILRRYSRHFAMLYFSGLGLSVSDSANPVHHYGHVQGGYSVPLIITASDITSHQPVSR
                     KISARHFAGIFQWMTGICTENIPPFNPLTDEDN"
BASE COUNT          827 a          770 c          723 g          818 t
ORIGIN      
        1 cgccagtact attgattgtt atcaatacct aatccgtaca atcttttcct ccatttcttc
       61 ttaacatatt tcactctatg aaagataaaa ctgataccca cctacagttt tgggtcacca
      121 acaaaaaaca atgttcgcca gatattccac cactggcaca gttcaaccct gcttcaaaaa
      181 ttgacttatt cattgtgctc attttcgtag tccgttcagg ttgaataaaa ctcccttagt
      241 caaatgagga aagactgaga ggttattact ttagcttacg ttatagctgt tttcctttgc
      301 agtatttctt tgcttttctg tataccttta tacctgttat accagatcaa aaaacaagca
      361 atccacataa caaaacgcgt tttgttactg atgtcacaaa ttgagcatat ttttgtagct
      421 gatagtttgt tacaaacacc agaaccaaga tgcctatttc gtttcagcat caactgttaa
      481 attatctggc tgccaacgag aatactgagc ttttttgtcc ccaagactga ccgtcaacgc
      541 cacaatcttt gactgtttga cattgtcatg cccgcaggcc agcggatcta aagacattag
      601 actgcttgca tcagccagtt gcgtatctgg ctatgtccct taattggtaa gggttcgagg
      661 aactcaacat taacgcattt ttcagcgcat caccgacagt gtatatgaac gattgcactc
      721 tgacgatggt tgatttattt ggttgcgtag gggctagcta gtctggtacc ggaggctgga
      781 tctgaagtac tgaccccagg aataacgccg ggctgactca tattctgacc cattgtcagc
      841 ccgcgtgttt actgctctag ctcatttcct gttgtcaggc gttcaattcc caactgcagg
      901 ttatggcccg gtgcacaaac acctgacggc aggtatcact tacccgaaga aggtaaagcc
      961 ctcaagaggc agtaactcag ccaacctctc ctccggccac tcaggcagac gcgtcaggac
     1021 gtccgtcagc caagcatgtg gctccagact gcggttccca gaaggctcat tatctgtgcc
     1081 gcgcggttcc cggccatcag tgaaccagcg aacaaccagg cctttcgtcc cataacgacc
     1141 ggacggatag cacgctcgca gatattgttg tgtgtgcctt gaaatgcacg atctttccgt
     1201 cggtgaaagt ccgccccggc taactctcgc tccgactgga aggacaagaa gcggtccaga
     1261 aggtaacaaa agggctgaat ctctccctga aacgacttct tcaggaagtc aacaggttat
     1321 gcgggccgta acgtgagtga acactgagca gccatcgaaa tgccgtcgag ggtgctgaca
     1381 cgccacaata acggggaagg ccgacgatga ccgggaagag agtgagaaag gcatcggtca
     1441 tacccaccgg ggttgtggtg tcagcatgca taacaattga tcgatcgtgc aacaagagaa
     1501 gtccataacg atgtctgtcc gggtgacggg caacaacgcc tcgcgagggg gtgaatcggt
     1561 tgttatggat ggcagatggg gtgtagtagt gaaaatactg agtaatgtca gaggagcgaa
     1621 ggccccctct tgcagtacca gtgtaacatg caggaagagc ccggggattg ccatcagcct
     1681 cacaaccccg ctcgaaccgg ttcagaacct acagaaaaca ctacaggaga aagcaaagtg
     1741 aaccgccccg tgtttcctgt ttatcatttt ctggtcagtg ccgcgattct ggtattcgtg
     1801 gtgattttct ggcggacaca ccatcgcgac caccgtaact ggctggctct acggctattc
     1861 gtattatgct ctgtaaaccg ttggccattg cggatggtta aaggaacagt tgtggggaca
     1921 actgacacat tacgtgaaat gcagcgttat gaacaactga gtcagcacgg ggctgacaac
     1981 tggaaaatcc agccgggagc accgttatat gatacgattg ttattgtcac tggtgagagt
     2041 gtgcgcaggg attatatgtc agtgtatggc tatcccgtac caaccacacc gtggctgaat
     2101 acggcacccg gtttatttat tgacgattat acctcgacag cctccagtac agtgtcttcc
     2161 ctgagccgga cactgattta tgactatgag cagaaccctg attccggcaa caatgtggta
     2221 gcgctggcag caaaagcagg atacagcaca tggtggatat ccaatcaagg aaaactgggg
     2281 aagcatgaca cacgcatctc tgttattgct tctgatgcgg agcatgccac tttcctcaag
     2341 aaaggcagct tcgcttcccg taaaacagat gacaaactgt tgttacagga aacagaacgt
     2401 gcgctggcgg atacatcctc tccgaagata attttcctgc acatgatggg ttctcatcca
     2461 aatccgtgtg acagccttaa ctcctggccg aataattacc tggagcagta tccccgaaag
     2521 attgcctgtt acctcgccag catcagtaaa ctggataact ttcttggcca gcttgatggt
     2581 atccttcgcc ggtactcacg tcatttcgcc atgctttact tttctggcct tgggctgtcg
     2641 gtcagcgaca gtgccaatcc tgttcatcat tatggtcatg tgcagggagg ctacagtgtg
     2701 ccactgatta ttaccgccag tgacataacg tctcatcagc ccgtcagtag aaaaatcagt
     2761 gcccgtcatt tcgcaggtat ttttcagtgg atgaccggta tttgtactga aaatataccg
     2821 ccattcaatc cgctgacaga cgaagataat taacctgtta tggtttttaa gggtgagagg
     2881 aatataccgg cagacagttt gaaacctcag ccacttattc ttcctgatca caggtaatca
     2941 tatatggcaa actgtaatac agtcgcttgt actgaaaagt attctgtata aaatatccgt
     3001 attcatatca gcacaaggga ccatcaaatc cctgcagcac tccttacgtc agttatcccg
     3061 catcaccctg tgagtgcagg atttttatga ggttactttt ttattgaatg tatataaaac
     3121 gcggattctg ataatccc
//
LOCUS       DADSZD010000284         3065 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.286, whole genome shotgun sequence.
ACCESSION   DADSZD010000284 DADSZD010000000
VERSION     DADSZD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.286"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            322..2292
                     /locus_tag="J1Z28_004377"
     CDS             322..2292
                     /locus_tag="J1Z28_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBA7719132.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGIL
                     VLVDGVRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDV
                     QFWYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDR
                     YKSFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDIQGNYQSDYVDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHRGVYAGWRAALSAAATHVKKGDPCQDGYAIHSFSLSYKPVSVKGFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVNLYTRYQW"
     gene            2311..>3065
                     /locus_tag="J1Z28_004378"
     CDS             2311..>3065
                     /locus_tag="J1Z28_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HBA7719133.1"
                     /translation="MMKNTGYILALCLAASGQTLAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRDFFETPRLYNGKETITLKPTSTDYVYKTDSAGNGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVTYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLNVLYKTSPLAGATLELDSVSYLKSSRHTHATEHKHSAHKAELTFVSN
                     EDGIITIPSLHAGQWLAKVKNKKVFPDKSLCDET"
BASE COUNT          876 a          705 c          731 g          753 t
ORIGIN      
        1 tatctttgag taaacaagcc gtcatttcac atgagagcat ccgtgcccaa ctggtttcta
       61 tatactacga tgatgaatgt atgagttaac gctcattcgt catagaacag atttgcagct
      121 tctcctgtac ttgaataaac gttaactaat tccgtattgc tggttcttta tcggtagaaa
      181 aatcaatatc catagcatca cattgcagag cgaccgggta ttagagtcgc tattcactgg
      241 ttatcactga acgctgcgta atagtggttg cacagacaaa atgttatgtt ataacattac
      301 tatattggca tatattttcg aatgtatatg aacgtaatca gaactgtcat ttgtacatta
      361 atcatacttc cggtggggtt acaggcagcg acaagtcatt cttctatggc taaagataca
      421 atcaccgttg tcgcgacagg aaatcagaac acggtatttg aaacgccgtc gatggtcagt
      481 gtcgtcacga atgacacacc gtggagtcag aatgcggtta catcggctgg gatgctgaaa
      541 ggtgttgccg gtctcagcca gactggtgca ggacggacca atgggcagac ctttaatcta
      601 cgcggctatg acaaaagcgg gatacttgtt cttgttgacg gcgtccgcca actcagtgac
      661 atggcaaaaa gcagtggcac ttatctggat ccggcactcg tcaaacgtat cgaagttgtc
      721 cgcgggccaa actccagtct gtacggcagt ggcgggctgg gaggtgtagt ggacttcaga
      781 actgccgatg cagcagattt tcttcccccc ggagagacaa acggtttaag tctgtgggga
      841 aatatcgcca gtggtgacca cagcacaggc tcggggctca cctggttcgg taaaaccgga
      901 aaaacagatg cgctcctttc tgtcattatg cgtaaaagag gtaatatata tcaaagtgat
      961 ggtgagcgcg cacctaacaa ggaaaaacct gcagccctgt ttgcgaaagg ctctgtcggt
     1021 ataacagaca gtaacaaagc aggtgccagc ttgcgtctct accggaataa caccactgaa
     1081 ccgggcaatc ccactcagac acatggtgac agcggcctgc gtgacagaaa aacagtacaa
     1141 aatgacgtac agttctggta ccagtacgct cctgtggata acagcctcat caatgtaaag
     1201 tcaacgttat atctcagtga tatcactatc aagacaaacg gtcacaacaa aacggcagaa
     1261 tggcgaaaca acagaacctc cggtgttaat gttgtcaaca ggagtcatac tctgattttt
     1321 ccgggagccc atcagttaag ttatggcgct gaatattacc gtcagcagca gaagccggaa
     1381 ggctctgcca cactatatcc ggaaggaaac attgacttta catcgttgta tttccaggat
     1441 gaaatgacaa tgaaaagcta cccggttaac attatcgtcg gctcccgcta tgaccggtac
     1501 aagagcttca atccccgtgc cggagaactg aaagccgaac gcctgtcccc aagggcggct
     1561 atttcagtct caccgacaga ctggctgatg atgtacggct ccatatcctc tgcattccga
     1621 gcgcccacaa tggcagaaat gtacagggat gatgtacatt tttaccgcaa gggtaaaccc
     1681 aattactggg ttcctaacct taatctgaaa ccagaaaata acatcacccg tgagattggc
     1741 gcaggtattc aactggatgg cctgcttaca gacaatgacc ggctgcagtt aaaaggcgga
     1801 tatttcggaa cggatgccag aaactatatt gccacgcgcg tggatatgaa acggatgcgt
     1861 tcttattctt ataatgtatc ccgggcccgt atctggggat gggatattca gggaaattac
     1921 cagtctgatt atgttgactg gatgctttct tataaccgga cggaaagtat ggatgccagc
     1981 agcagggaat ggctgggctc cggcaatcct gacacactta tcagtgacat cagcatacct
     2041 gttggtcata gaggcgttta tgcaggatgg cgtgctgcac tttcggcagc agccacgcat
     2101 gtgaaaaaag gcgacccctg tcaggatggc tatgccatac attccttttc actgtcttat
     2161 aagccagtaa gtgttaaagg ctttgaggcg tcagtaactc tggataatgc cttcaacaag
     2221 cttgccatga atggcaaagg tgtgccgctt tcaggcagaa ccgtcaatct ttatacccgt
     2281 tatcagtggt aacatctcag gaaataaatc atgatgaaaa atacaggcta tatcttagct
     2341 ctttgtctgg cagcatcggg ccagaccctg gcccacgatg tctggattac cggtaaacag
     2401 gcagagaaca acgttactgc agagattggt tatggccata atttcccctc aaagggcacg
     2461 attcctgaca gaagggattt ctttgaaact ccccgacttt ataacgggaa agagacaata
     2521 acgctgaagc caacgtccac ggattatgtc tataaaactg actctgcggg caacggtaat
     2581 agctacgttc tgtcaacgaa aatgaaaccg ggatactggt cgagaacctc atcaggatgg
     2641 aagcctgtca gccgggaggg cagaaatgat gtgacatact gtgagtttgt gactaaatat
     2701 gcaaaatctt ttattcctgg tgaacagcag atgccagcac aactctatca gtctccaaca
     2761 ggggatgagc ttgaaatcat tccattatcc gatatcagtc gtttcggtga agatgtgaag
     2821 ctgaacgttc tgtataaaac gtcccctctt gccggagcca ctctggagct tgactctgtc
     2881 agttatctga aatcatcccg tcacactcat gcaacagagc ataaacattc ggctcataaa
     2941 gcagaactta cctttgtaag taatgaggac ggtatcatca ctataccttc ccttcatgcc
     3001 gggcaatggc tggctaaagt caaaaataag aaagtatttc cggacaagag cctgtgtgat
     3061 gaaac
//
LOCUS       DADSZD010000285         3037 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.287, whole genome shotgun sequence.
ACCESSION   DADSZD010000285 DADSZD010000000
VERSION     DADSZD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.287"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            620..1597
                     /locus_tag="J1Z28_004379"
     CDS             620..1597
                     /locus_tag="J1Z28_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HBA7719134.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKFFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(1882..2622)
                     /locus_tag="J1Z28_004380"
     CDS             complement(1882..2622)
                     /locus_tag="J1Z28_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HBA7719135.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLNGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(2743..2931)
                     /locus_tag="J1Z28_004381"
     CDS             complement(2743..2931)
                     /locus_tag="J1Z28_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HBA7719136.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPISPKNGKPAISPRHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT          757 a          757 c          768 g          755 t
ORIGIN      
        1 gggggcacag tgaaaccgat attgtacaga aagtacagca attacagagt gttacatgca
       61 gagtggctat cagatttctg ctccggtata gcaatcaaaa aaaacagtgc tcgccgcaga
      121 ccggtgaccg ggcgaagccc gcgaaccggc gaggaagcgc aatgtatctg tagttacagt
      181 gagcaggtac gccctgaagc cctttcataa aacggttgtg tgcgctgtcc ttctggaatt
      241 atttatccac atatccggag gcccgatcca gtaaactaga tccatgaatg atcaataaag
      301 gatccattaa agatccccat attgctgtac accttgtcat taatggtccg ggagcacgat
      361 cacataagct atggcatgtt actgataaac tgtaacaagt taatgataag ctgtattaag
      421 taatgcatat actgcagtaa gttaatgaca taaactatgg tcagtatgcc agactcagtt
      481 gttaaataca ggctacaggt ttttcttcag tcagttagcg gagttctgac acacgatttt
      541 ctgtttattc ttttactgtc cacaggctgg aggctttctg gaaaacgaaa attcagacat
      601 caaaaaactg ttcggcgagg tggataagtc gtccggtgaa ctggtgacac tgacaccaaa
      661 caataacaac accgtacaac ctgtggcgct gatgcgtctg ggcgtttttg taccgaccct
      721 taaatcactg aagaacagta aaaaaaatac actgtcacgt actgatgcca cggaagagct
      781 gacacgtctt tccctggccc gtgctgaggg attcgataag gttgagatca ccggcccccg
      841 cctggatatg gataacgatt tcaagacctg ggtggggatc attcattcct ttgcccgcca
      901 caacgtgatt ggtgacaaag ttgaactgcc ttttgttgag tttgcaaaac tgtgtggtat
      961 accttcaagc cagtcatccc gcaggctgcg tgagcgcatc agcccttccc tgaagcgcat
     1021 tgccggtacc gtgatctcgt tttcccgtac cgatgagaag cacacccggg aatacatcac
     1081 ccatctggta cagtcagcct actacgatac tgagcgggat attgttcagt tacaggccga
     1141 tccccgcctg tttgaactgt accagtttga cagaaaagtc cttctccagc ttaaggcgat
     1201 taatgccctg aagcgacggg agtccgccca ggcactctac acctttatag agagcctgcc
     1261 ccgggatccg gcaccgatat cgctggcgcg gctgcgtgca cgcctcaatc tgaagtctcc
     1321 tgtattttcc cagaaccaga cggtcagacg ggcaatggag cagttgcgcg agattggata
     1381 tcttgattac acggagatcc agcgggggcg gacaaaattc ttctgtattc actaccggcg
     1441 tccccggtta aaagcgccga atgatgagag taaggaaaat ccgttgccac cttcacctgc
     1501 ggaaaaagtc agtccggaga tggcggagaa gcttgccctg cttgagaaac tgggcatcac
     1561 gctggatgac ctggaaaaac tcttcaaatc ccgctgaaca taaactgtag tcagtgaaga
     1621 gcgctccttt actgactaca gcttatatta accggtgcag tgagtggtct gctcactgca
     1681 gtttatattc tgtttcctgc agtgctgtct gtagctgagc tgccatctgc ctgtccctta
     1741 cgtaagtgat cccgtaacct gatgctgagg cattgctccc ttcataaaac atgacttact
     1801 cactacagct tatatacatg ctccagctta tgttatgtct gttctgctga ccacagctta
     1861 tataaggaag cgcgtatgcc gtcacctcag gggaggcagt gtacgcagga tctccgcagc
     1921 atcccgcccg tcacctgtga aaggcactgc cagcgtggca gccatatcca gagcaaacac
     1981 tctggtataa acctccatcg aacgtggatc cctgtgacca gccagtgcct ggatgacttt
     2041 ccggggctgg cggtgataga gcatgtgcat gatatagctg tgccggaagg tgtgtggtgt
     2101 gactggaatc gaaaagtgta ctccgtcagc ttcggcccgt ctgacagcct gcttcagcca
     2161 gttgcgcatg gtttcgtcgg tcacggccca taatggttca cgacgacggg gccgggtggt
     2221 gatcatccag ctttccatct gcctgacata gcttatatct gtcagcggaa ccaggcgcac
     2281 ttcatctttt ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga tccgcacaaa
     2341 cggtcttact ccattcaggt caaatgattc cggcgtcagc atccgggctt cgccaatacg
     2401 cattccggta ttccagaggg tggcgaacag catatggtga cgctgatccg gcatatagaa
     2461 aagaagggca ctcacttccg gggccagcag gtaggccggt gtggcccccg tggaagtggc
     2521 cattcttctc agggagagcg ctgttgcaaa atccaccccc ggcgcaatgg gtaacaggga
     2581 gacgcgttct ggtgaattct gcaggggaat gacattgttc atgagttatt tttcacgtag
     2641 cacacgcact gtttatggat acaggtattc tgacctgaac gcccggatcc ttccacgaca
     2701 ataaatcgca gcacgcggat ccttttgttt gcttatcacg cattattgcg ggtaaaacca
     2761 aatacgtaaa ggcgcatttt gccgcaggga tcccgtctta agccagtcgt ggcgcgggct
     2821 gatggctggt ttgccgtttt tgggagaaat ggggtaatgc caggaacagg cagatacgga
     2881 gaaaaatcct gcagataacc ttctacgaca actgaaggtg tctgacgcca tattcttagc
     2941 tacttctcag ttttaaggcg gcttagtcac aacataaggt tatgcataat aagcatattt
     3001 tattatgtta tggtcttgtt catgcatgaa ctgttat
//
LOCUS       DADSZD010000286         3000 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.288, whole genome shotgun sequence.
ACCESSION   DADSZD010000286 DADSZD010000000
VERSION     DADSZD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.288"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            167..316
                     /locus_tag="J1Z28_004382"
     CDS             167..316
                     /locus_tag="J1Z28_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBA7719137.1"
                     /translation="MEKVISHTRYKLTPAEMEAFNSAVDHRLAELTMNKLYDRVPASV
                     WKYVI"
     gene            1062..1631
                     /locus_tag="J1Z28_004383"
     CDS             1062..1631
                     /locus_tag="J1Z28_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA7719138.1"
                     /translation="MTYKYNPFWQQRIRETVRHALAVHPRLTALRVDLRLPDISAETD
                     AAVISRFVNALKARINAYQKRKRREDKRVHPTTLHYAWAREFGERKGKKHYHLLLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDAGCHATLATFPEHPALWLTRDDDAGFQ
                     QLLERADYLAKESTKAYGTGERNFGCSRG"
     gene            1799..2181
                     /locus_tag="J1Z28_004384"
                     /pseudo
     CDS             1799..2181
                     /locus_tag="J1Z28_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2178..2603
                     /locus_tag="J1Z28_004385"
                     /pseudo
     CDS             2178..2603
                     /locus_tag="J1Z28_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2804..>3000
                     /locus_tag="J1Z28_004386"
     CDS             2804..>3000
                     /locus_tag="J1Z28_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA7719139.1"
                     /translation="MVSINHTRESSPYLSVPQDDFCIVNPGGCIVHFPANGRDILDIN
                     DPDFPEQYQRLLARLDYLTKL"
BASE COUNT          709 a          752 c          819 g          720 t
ORIGIN      
        1 gagaccggtt aagtggtggt acgggagtaa tgagtttggg gagggtacca gaatgtcagt
       61 ctggtacggt atgctgacag tatcacgtta attgcagcat aaagtgatga gaggctaatg
      121 gaagttaaaa ccagagcagt tccgtcgttg ttcatcccgg gagacactgg aaaaagtgat
      181 ttcccacacg cgttataagc ttactccagc ggagatggaa gccttcaact ctgcggtcga
      241 tcaccgactg gcagaactga caatgaacaa gctttacgat cgtgttcccg cttcagtctg
      301 gaaatatgtc atctgagcgt tcggtaatca caactgcatc gcgccaggtg ttatccgctt
      361 tgtgattctt attggctctt ctcaggttat taaaagctga atatgttcct gttgtcggga
      421 accaggataa taccgggaac gttaagcggt atagcgacaa caaagaattc gaaatcaaaa
      481 gggaatggtg tacggaggtg taggcggtaa tgggccgatt ttcgggcatt accgacctct
      541 gctgaggttt tctcgtattt atcggggagc tggtgcgtta ctgtcactgt ctggcacagc
      601 aaacgcagag gtggcggggc gctctgctcc gctgactccg gctaatctct ccccttttac
      661 ctactgcaac agtacaaata cactgacgcc tgaaacgaca gccagaatca gcacaggcag
      721 taacgtcagt agacaaagca gacgatcggt atttcggtaa taatcgaagg gatctgctga
      781 cacgtagccc gggcaacatt ccgtattatg agggatggcg ttatcacaat ccctgcacac
      841 cgttttgcgt atcaccatca cgtcctcctc acaatcttat ccgtaaccgg ctttttgcat
      901 taaaaaaaca acgctgaaaa ttcagccgtc agtccggaga cgacagtcgg gttatctcag
      961 agtgcctgag atattgcctg gttggagatc acttcaggga ctctgcgcat tttttacggc
     1021 gtggttaatc cgtatgaacg aaaccatacg gagatttaac catgacctac aaatacaacc
     1081 ccttctggca gcaacgtatt cgtgagacgg tgcggcacgc actggcggtg catccccgcc
     1141 tgacggcact tcgggtggat ctgcgtctgc cggatatatc cgcagagact gatgctgctg
     1201 tgatatcccg cttcgtcaat gccctgaaag ccagaatcaa cgcttaccag aagcgtaagc
     1261 gccgtgaaga taaacgcgta catcccacga ccctgcatta cgcctgggcc cgggaattcg
     1321 gggagcgtaa aggtaaaaaa cactatcatc tgctgttgct ggtcaaccgg gatacctggt
     1381 gtcgtgccgg tgattaccgc gctccggagt ctctggccgg gatgattaaa caggcatggt
     1441 gcagtgccct gggagtggat gccggatgcc atgccacgct ggcgaccttt ccggaacatc
     1501 ccgcactgtg gctgacgcgt gatgatgacg ccggctttca gcagctactg gaacgggctg
     1561 attatctggc gaaggagagt accaaagctt acggcaccgg tgagcgcaac tttggctgca
     1621 gtcgtggcta agcccgacag acgacttatc tgaccttagt gtgatgacgt gttcacagcg
     1681 accacactga ttttcccttc tgagactgaa ctgaaaaccg ccattcacac ctgaccacgc
     1741 tgcccgtacc gggacgactg ttgcctgcgt gtcgtctggc ggctgaggaa tatatactat
     1801 gtacgcagaa tcctttctcg cacttgatgg taacggacgt ctgacggggc ccgtaccgca
     1861 cagactgctc cttatgccca ctacacctgc cacttgtgtg gcagtgcact cagataccac
     1921 ccgcaatacg acaccgaacg gccctggttt gaacacactg acgacgggct gacagagcac
     1981 ggtcaacagt gtccttatgt cagaccggag cgcagggaga tacaactgat taaacgtctg
     2041 cagcaattcg taccggatgc cttacccgta gtacgtaaag ccagctggca ctgcaggcaa
     2101 tgtcaccacg attattatgg ggagcggtac tgcacacact gccagaccgg gcgcttcagt
     2161 gaggaggggg gagcagaatg aacggtataa aaacggggct ggggatcacg ccgggtgagc
     2221 acattatcag tgccgactct gcgctcagca gaaatatccg gcagtgtttc tgcctttcct
     2281 gtcatggccg gttgattctg cagacagatg ctcagggggc gtggtttgag cacgatttac
     2341 atgccctgag ctagcaacag aaggcggact gtgtggtgct gaatccggag aagtcccacc
     2401 cttacattga ggatatggcg atgtttttat cgccattacc ggtcgtgctg gagtggcatt
     2461 gtgtgatgtg tgagcagttt tttcatggga agaagttctg tgaggcctgt ggtacgggga
     2521 tttattgcag agcggtatgt acgaagtctg tttacagcta ccagccagac ttgtttgagg
     2581 actgcggagg tgcgagtact taatccgaaa tctccgctct gcgcagggcg ttcagaggta
     2641 tcgcaacctg cattttatcc ggtgtttcgc acctgccccg ttcgtgacac aaaggcgcga
     2701 caccggatat ccgcagtcct gcttacctga ataaaacccg gggtattgat tggtagtgtg
     2761 gtgcggaatc gtaccgtgat ctcgcattcg ccgaattcgc actgtggtgt caatcaacca
     2821 tacgcgagag tcctctccgt atttatcagt gcctcaggac gatttctgca tagttaatcc
     2881 agggggctgc attgttcatt tccctgctaa tggacgtgac atcctggata ttaacgatcc
     2941 tgatttccct gagcagtatc agcgtttatt ggcacgtctg gattatctga cgaagctcga
//
LOCUS       DADSZD010000287         2987 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.289, whole genome shotgun sequence.
ACCESSION   DADSZD010000287 DADSZD010000000
VERSION     DADSZD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.289"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            110..1351
                     /locus_tag="J1Z28_004387"
     CDS             110..1351
                     /locus_tag="J1Z28_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBA7719140.1"
                     /translation="MKWQGRAILLPGIPLWLIMLGSIMFITAFLMFIIVGTYSRRVNV
                     SGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKSTRNGIVTDNHRR
                     DIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQRAEEGIKIMKNN
                     MENYRYYQSKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQITTLESQIQTQAA
                     DFDNRIYQMELQRLELQKELVNTDVEGEIIIRALSDGKVDSLSVTVGQMVNTGDSLLQ
                     VIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPSEKFGQFSATVKTISRTPAST
                     QEMLTYKGAPQNTPGASVPWYKVIATPEKQIIRYDEKYLPLENGMKAESTLFLEKRRI
                     YQWMLSPFYDMKHSATGPIND"
     gene            1326..>2987
                     /locus_tag="J1Z28_004388"
     CDS             1326..>2987
                     /locus_tag="J1Z28_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HBA7719141.1"
                     /translation="MQQDRSMTNRNFRQIINLLDLRWQRRVPVIHQTETAECGLACLA
                     MICGHFGKNIDLIYLRRKFNLSARGATLAGINGIAEQLGMATRALSLELDELRVLKTP
                     CILHWDFSHFVVLVSVKRNRYVLHDPARGIRYISREEMSRYFTGVALEVWPGSEFQSE
                     TLQTRISLRSLINSIYGIKRTLAKIFCLSVVIEAINLLMPVGTQLVMDHAIPAGDRGL
                     LTLISAALMFFILLKAATSTLRAWSSLVMSTLINVQWQSGLFDHLLRLPLAFFERRKL
                     GDIQSRFDSLDTLRATFTTSVIGFIMDSIMVVGVFVMMLLYGGYLTWIVLCFTTIYIF
                     IRLVTYGNYRQISEECLVREARAASYFMETLYGIATVKIQGMVGIRGAHWLNMKIDAI
                     NSGIKLTRMDLLFGGINTFVTACDQVVILWLGAGLVIDNQMTIGMFVAFSSFRGQFSE
                     RVASLTSFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVAHMEPISLETNGLSYRYD
                     SQSAPIFSALSLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSG"
BASE COUNT          860 a          592 c          703 g          832 t
ORIGIN      
        1 taatgcaaat tttcgattta ttttccatca ttaatccaga taaacaacaa actaatagta
       61 tgcaaggagg cattatttgt ttcgccagga tgctttagaa aacagaaaaa tgaagtggca
      121 gggacgggca atattacttc ccggaatacc actgtggtta atcatgctgg gaagcattat
      181 gtttattacg gcatttctga tgttcattat tgttggtacc tatagccgcc gtgttaatgt
      241 cagtggtgag gtcacaacct ggccaagagc tgtcaatata tattcaggtg tacagggatt
      301 tgttgtcagg cagtttgttc atgaagggca gttgataaaa aaaggggatc ctgtttatct
      361 gattgacatc agtaaaagta cacgcaatgg tattgtcact gataatcatc gccgggatat
      421 agaaaaccag ttggttcgtg tggacaacat tatttcccgt ctggaagaaa gtaaaaaaat
      481 aacgctagat accctggaaa aacaacgtct gcaatacaca gatgcgttcc gtcgctcatc
      541 agacattata cagcgtgcag aggaagggat aaaaataatg aaaaataata tggagaatta
      601 cagatactat cagtcaaaag gactgattaa taaagatcaa ttaactaacc aagttgcatt
      661 atattatcaa caacaaaaca accttctcag tctgagcgga caaaatgaac aaaatgccct
      721 gcagataacc actctggaga gtcagattca gactcaggca gcagattttg ataatcgtat
      781 ctatcagatg gaactacaac gactcgaatt gcagaaagaa ctggttaaca ctgatgtgga
      841 aggcgaaatc attatccggg cgttgtctga cgggaaagtt gactccctga gtgtcactgt
      901 agggcaaatg gtcaataccg gagacagcct tctgcaggtt attcctgaga acattgaaaa
      961 ctattatctt attctctggg tcccgaatga tgctgttcct tatatttcgg ctggtgacaa
     1021 agtgaatatt cgttatgaag ccttcccctc agaaaaattt gggcagttct ctgctacggt
     1081 taaaactata tccaggactc ctgcgtcaac acaggaaatg ttgacctata agggagcacc
     1141 tcaaaatacg ccgggtgcct ctgttccctg gtataaagtc attgcgacgc ctgaaaagca
     1201 gataatcagg tatgacgaaa aatacctccc tctggaaaat ggaatgaaag ccgaaagtac
     1261 actatttctg gaaaaaaggc gtatttacca gtggatgctt tctcctttct atgacatgaa
     1321 acacagtgca acaggaccga tcaatgacta acaggaattt cagacaaatt ataaatctgc
     1381 ttgatttgcg ctggcaacgt cgtgttccgg ttattcatca gacggagacc gctgaatgtg
     1441 gactggcctg cctagcaatg atatgcggtc attttggtaa gaatattgac ctgatatatc
     1501 ttcgccggaa gtttaatctc tctgcccgtg gagcaaccct tgcaggaatc aatggaatag
     1561 cggagcaact ggggatggcc acccgggctc tttcactgga gttggatgaa cttcgagtcc
     1621 tcaaaacgcc gtgtattctc cactgggatt tcagtcactt cgtcgttctg gtcagcgtaa
     1681 agcgtaaccg ttatgtactg catgatccgg ccaggggcat aagatatatc agccgggagg
     1741 aaatgagccg atattttaca ggcgttgcac ttgaggtctg gcccggaagt gaattccagt
     1801 cggaaaccct gcagacccgc ataagtcttc gttcactgat taacagtatt tacggtatta
     1861 aaagaacgct ggcgaaaatt ttctgtctgt cagttgtaat tgaagcaatc aatctgctaa
     1921 tgccggtggg gacacagctg gttatggatc atgctattcc tgcgggggac agagggctac
     1981 tgacgctaat ttctgctgct cttatgtttt ttatattact caaagctgca acgagtacgc
     2041 tgcgcgcatg gtcttcactg gttatgagca cgctcatcaa tgtacagtgg cagtcggggc
     2101 tgttcgatca tcttctcaga ctaccgctgg cgttttttga acgccgaaaa ttaggtgata
     2161 tccaatcacg ttttgactcc cttgacacat tgagggccac atttaccacc agtgtgatcg
     2221 ggttcataat ggacagcatt atggttgtcg gtgtttttgt gatgatgctg ttatacggag
     2281 gatatctcac ctggatagtt ctctgcttta ccacaattta catttttatt cgtctggtga
     2341 catacggcaa ttaccgacag atatcagaag aatgtcttgt cagggaggcc cgtgccgcct
     2401 cctattttat ggaaacatta tatggtattg ccacggtaaa aatccagggg atggtcggaa
     2461 ttcggggggc acactggctt aatatgaaaa tagatgcgat aaattcgggt attaagctaa
     2521 ccaggatgga tttgctcttc ggaggaataa atacctttgt taccgcctgt gatcaggttg
     2581 taattttatg gctgggagca ggccttgtga tcgataatca gatgacaata ggaatgtttg
     2641 tagcgtttag ttcttttcgt gggcagtttt cggaaagagt tgcctctctg accagttttc
     2701 ttcttcagct aagaataatg agtctgcaca atgagcgcat tgcagatatt gcattacatg
     2761 aaaaggagga aaagaaacct gaaattgaaa tcgttgctca tatggagcca atatccttgg
     2821 aaaccaatgg tttaagctat cgttatgaca gtcagtcagc accgatattc agtgctctga
     2881 gtttatcagt agctccgggg gaaagtgtgg ctataactgg tgcttccggt gcgggaaaaa
     2941 ccacattaat gaaagtacta tgtggactat ttgaacctga tagcggg
//
LOCUS       DADSZD010000288         2952 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.290, whole genome shotgun sequence.
ACCESSION   DADSZD010000288 DADSZD010000000
VERSION     DADSZD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.290"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..2796
                     /gene="ag43"
                     /locus_tag="J1Z28_004389"
     CDS             <1..2796
                     /gene="ag43"
                     /locus_tag="J1Z28_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBA7719142.1"
                     /translation="GTLVVASELARSRGKGAGVAVALSLAAVTSVPALAADTVVQAGE
                     TVNGGTLTNHDNQIVLGTANGMTISTGLEYGLDNEANTGGQWIQNGGIANNTTVTGGG
                     LQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHEGGIATGTVINDKGWQAV
                     KSGAVATDTVVNTGAEGGPDAENGDTGQFVRGNAIRTTINKNGRQIVAAEGTANTTVV
                     YAGGDQTVHGRALDTTLNGGYQYVHNGGTASDTVVNSDGWQIVKEGGLADFTIVNQKG
                     KLQVNAGGTATNVTLKQGGALVTSTAATVTGSNRLGNFTVENGNADGVVLESGGRLDV
                     LEGHSAWKTLVDDGGTLAVSAGGKATDVTMTSGGALIADSGATVEGTNASGKFSIDGI
                     SGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGSLSGRTQLSKGASMVLNG
                     DVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHKLTTSNLTGQGGTINMRV
                     RLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQGIRVVDAQNGATTEEGA
                     FALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRS
                     HQSGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESNGSYGFVRLEGDLLRTEVAGM
                     SLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLVHTSSGLWADIVAQGTR
                     HSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAG
                     YVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAKHSVRELPVNWWVQPSVI
                     RTFSSRGDMSMGTAAAGSNMTFSPSQNGTTLDLQAGLEARVRENITLGVQAGYAHSVS
                     GSSAEGYNGQATLNVTF"
BASE COUNT          676 a          775 c          913 g          588 t
ORIGIN      
        1 ggcaccctgg tggtggcttc cgaactggca cgctcacggg gaaaaggcgc cggtgtggcg
       61 gttgctctgt ctcttgctgc tgtcacgtca gtcccggcac tggctgctga cacggttgta
      121 caggcgggag aaaccgtgaa cggcggaaca ctgacaaatc atgacaacca gattgtcctc
      181 ggtacggcca acggaatgac catcagtacc ggtctggagt atgggctgga taacgaggcc
      241 aataccggcg gacaatggat acaaaatggc ggtatcgcca acaacactac tgtcaccggt
      301 ggtggtcttc agagagtgaa tgccggagga agcgtttcag acacggttat cagtgccgga
      361 ggcggacaga gccttcaggg gcaggcagtg aataccactc tgaacggcgg tgagcagtgg
      421 gtacatgaag gcgggattgc aacgggtacc gtcattaatg ataagggctg gcaggccgtc
      481 aaatccggcg cagtggcaac cgacacggtt gtgaataccg gcgcggaagg gggaccggat
      541 gcagaaaatg gtgataccgg gcagtttgtt cgcggaaatg ccatacgcac caccatcaat
      601 aaaaatggtc gtcagattgt ggctgctgaa ggaacggcaa ataccactgt ggtttatgcc
      661 ggcggcgacc agactgtaca tggtcgcgca ctggatacca cgctgaatgg ggggtaccag
      721 tatgtgcaca acggcggtac ggcgtctgac actgttgtga acagtgacgg ctggcagatt
      781 gtcaaggaag gtggtctggc ggatttcacc atcgttaacc agaaaggcaa actgcaggtg
      841 aacgccggtg gtacagccac gaatgtcacc ctgaagcagg gaggcgcact ggtcaccagt
      901 acggcggcaa ccgtcaccgg cagcaaccgt ctgggcaatt tcactgtgga aaacggtaat
      961 gctgacggtg ttgttctgga gtccggtggt cgcctggatg tactggaggg ccattcagcc
     1021 tggaaaacac tggtggatga cggcggtacc ctggcagtgt ctgccggtgg taaggcaaca
     1081 gatgtcacca tgacatccgg tggtgccctg attgcagaca gtggtgccac tgttgagggg
     1141 accaatgcca gcggtaagtt cagtattgat ggcatatccg gtcaggccag cggcctgctg
     1201 ctggaaaatg gcggcagctt tacggttaat gccggaggac tggccagcaa caccactgtc
     1261 ggacatcgtg gaacactgac gctggccgcc gggggaagtc tgagtggcag aacacagctc
     1321 agtaaaggcg ccagtatggt actgaatggt gatgtggtca gtaccggcga tattgttaac
     1381 gcaggagaga ttcgctttga taatcagacg acaccggatg ccgcattgag ccgtgctgtt
     1441 gcaaaaggcg actccccggt aacgttccat aaactgacca ccagtaacct caccggtcag
     1501 ggtggcacca tcaatatgcg tgttcgcctt gatggcagca atgcctctga ccagctggtg
     1561 attaatggtg gtcaggcaac cggcaaaacc tggcttgcgt tcacaaatgt cggaaacagt
     1621 aacctcgggg tggcaaccac cggacagggt atccgggttg tggatgcaca gaatggtgcc
     1681 accacagaag aaggtgcgtt tgccctgagt cgcccgcttc aggccggcgc ctttaactac
     1741 accctgaacc gtgacagcga tgaagactgg tacctgcgca gtgaaaatgc ttatcgtgct
     1801 gaagtccccc tgtatgcctc catgctgaca caggcaatgg actatgaccg gattctggca
     1861 ggctcccgca gccatcagag cggtgtaagc ggtgaaaata acagcgtccg tctcagcatt
     1921 cagggcggtc atctcgggca cgataacaac ggtggtattg cccgtggggc cacgccggaa
     1981 agcaacggca gctatggctt cgtccgtctg gagggtgacc tgctcagaac agaggttgcc
     2041 ggtatgtcac tgacgacagg agtgtatggt gctgcaggcc attcttccgt tgatgttaag
     2101 gatgatgacg gctcccgtgc cggcacggtc cgggatgatg ccggcagtct gggcggatac
     2161 ctgaatctgg tacacacgtc ctccggcctg tgggctgaca ttgtggcaca gggaacacgc
     2221 cacagcatga aagcgtcatc ggacaataac gacttccgcg cccggggctg gggctggctg
     2281 ggctcactgg aaactggtct gcccttcagt atcactgaca acctgatgct ggagccacaa
     2341 ctgcagtata cctggcaggg actttccctg gatgacggcc aggataacgc cggttatgtg
     2401 aagttcgggc atggcagtgc acaacatgtg cgtgccggct tccgtctggg cagccacaac
     2461 gatatgaact ttggtaaagg cacctcatcc cgtgacaccc tgcgcgacag tgcaaaacac
     2521 agtgtgcgtg aactgccggt gaactggtgg gtacagcctt ctgttatccg caccttcagc
     2581 tcccgggggg acatgagcat gggtacagcc gcagccggca gtaacatgac gttctcaccg
     2641 tcccagaatg gcacgacact ggacctgcag gccggactgg aagcccgtgt ccgggaaaat
     2701 atcaccctgg gcgttcaggc cggttatgcc cacagtgtca gcggcagcag cgctgaaggt
     2761 tataacggtc aggccacgct gaatgtgact ttctgataat caggtcactc cccttcaggc
     2821 cccggtcatc atgaccgggg ccttttactt acagggagca acagatatgc cgggttgtac
     2881 atcacgttta ttgcccgaag gccctttctc acgcaaccag gctcttgccg tcacaacggc
     2941 ttaccttaat gt
//
LOCUS       DADSZD010000289         2947 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.291, whole genome shotgun sequence.
ACCESSION   DADSZD010000289 DADSZD010000000
VERSION     DADSZD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.291"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            75..899
                     /gene="rhaD"
                     /locus_tag="J1Z28_004390"
     CDS             75..899
                     /gene="rhaD"
                     /locus_tag="J1Z28_004390"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBA7719143.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLMLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            909..1223
                     /gene="rhaM"
                     /locus_tag="J1Z28_004391"
     CDS             909..1223
                     /gene="rhaM"
                     /locus_tag="J1Z28_004391"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBA7719144.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(1264..2658)
                     /locus_tag="J1Z28_004392"
     CDS             complement(1264..2658)
                     /locus_tag="J1Z28_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBA7719145.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT          791 a          763 c          638 g          755 t
ORIGIN      
        1 tgagtgcctt atccggccta cagatcgtac atccggcaac aggcacggac gtccaattaa
       61 caggaacaca gaacatgcaa aacataaccc agtcctggtt tgtccaggga atgatcaaag
      121 ccaccaccga tgcctggctg aaaggctggg acgagcgcaa cggcggcaac ctgacgctgc
      181 gcctggatga cgccgatatc gcaccatatc acgacaattt tcaccaacaa ccgcgctata
      241 tcccgctcag ccagcccatg cctttactgg caaatacacc gtttattgtc accggctccg
      301 gcaaattctt ccgtaacgtc cagcttgatc ctgcggctaa cttaggcatc gtaaaagtcg
      361 acagcgacgg cgcgggctac cacattcttt gggggttaac caacgaagcc gtccccactt
      421 ccgaacttcc agctcacttc ctttcccact gcgagcgcat taaagccacc aacggcaaag
      481 atcgggtgat catgcactgc cacgccacta acctgatcgc tctcacctac gtgctggaaa
      541 acgacaccgc ggtcttcact cgccaactgt gggaaggcag caccgagtgt ctggtggtat
      601 tcccggatgg cgttggcatt ctgccgtgga tggtacccgg cacggacgaa atcggccagg
      661 caactgcaca agagatgcaa aaacattcgc tgatgttgtg gcccttccac ggtgtgttcg
      721 gttccggacc gacactggat gaaaccttcg gtttaatcga caccgcagaa aaatcagcac
      781 aaatattagt gaaggtttat tcgatgggcg gcatgaaaca gaccatcagc cgtgaagagt
      841 tgatagcgct cggccagcgt ttcggcgtta caccactcgc cagtgcgctg gcgctgtaag
      901 gagcaaacat gatccgcaaa gcctttgtca tgcaggtaaa ccccgacgcc cacgaagagt
      961 atcagcgtcg gcataatccc atctggccgg aactggaagc agtgctgaaa tctcacggtg
     1021 cgcataacta cgccatctat ctcgacaaag cgcataatct gctatttgcc acggtagaga
     1081 ttgaatctga agaacggtgg aatgcggttg ccagtacaga tgtttgccaa cgttggtgga
     1141 aatatatgac cgacgttatg cccgctaatg cggataacag cccagtcagt agcgaactgc
     1201 aagaagtgtt ttacctgcct taatgagttg cggtgccgga aaatatccag caccgccctt
     1261 tcatcagaag aagtatttaa agcgaacgcg tgcgccgtaa cggtcatcac tatcgtcgcc
     1321 gtgtatctta tcgccgtcgc tatccagaat ggagtaatac gtgccgaggt aaatattaaa
     1381 gttctccata tccatcacat tagcgaactg ataagacgca tgaatggtgt gaatgttgta
     1441 tgttccttca tcatcaatcc agcaatcgtt atcacaaacc acgccactaa attcatcaat
     1501 cttattatgt gcatagatat aacccagctc gaaacgtttc cacagcgcgt taatccctgc
     1561 cgtgaaatct ttttcattat tagcatctaa ataggcggta ttaagattaa ccacgatgcc
     1621 attttccgga tcggttttca ggccattcca ggtcatactc atgccataac cggtacgatc
     1681 ggactgatcg acaaaattac ccttgctatc ggtataacca taagcattat ttaccacatt
     1741 cgcttccatc gctgcggaaa cggtgaattc ttccgtcggc gaccaggcaa ttaccggacg
     1801 cagataagca acatttttct gctgttccat atcgcgtcca tgataattac cgtcgttata
     1861 taaagatgtt ccgtcttcca gtaacgtgtt taattcaaaa taccagttat cgagttgttt
     1921 gctgacgagg aaattaccgc cagcgttaga acgcccgcgg ccctctttca tcatatagat
     1981 ataaccgctg ccatcgtcat aaagatcgtt cgcagtatta ccggaatgtt caacaaaggt
     2041 atcctgattc agcgggaaca tatcgtaagc ttcaaaacgg cctactttga ccttccagtc
     2101 gttttctttg ccaaagaaga aaaccgcatc atcgatattt actgaaccgt gcatatcccc
     2161 cagcggttgt gcggagaacc cagcgaaata gccattatcc agttttcgca taccatcaaa
     2221 acctaacaga atacgaccat tgagattcca ctgttcatta gtgggatcgc tattcacatc
     2281 agcgttggtc attgccagca ggccatgatt actttccgca tccatattaa attctacgtc
     2341 accgtagatt tttaaatcgc cataccctga aagcaggaga ttaggcgggg tggcgttttt
     2401 cttctctgac tccgtcgttg attgaccgtt taccggcgtg ttgccctgtg cggttttaat
     2461 ttcgcggatc tcggcggctt gttgtgtctg aagctgttta acctgcccct ctgcttttgc
     2521 tgcccgcatt tctgccgctt ccaggcgttc ttccagcaat gccatgcgtt gttccagcgt
     2581 taaatgctgt gtttttgcca taccggtact acatgccaat aataaaccga tggctaaaga
     2641 taatgtcgat tttttcatta tttacgttcc ctgaaataaa ggagacccta cgctttccaa
     2701 aaggaatacg tatatagaga aatataaggt gtgcactttc gaatttttaa tccaacttca
     2761 gcaggccgat ggatagtatc ccgccgcata atatgagtaa atccgctgat ttcctggaac
     2821 gtgaggaata taacaatagt gccgttttta actaaatatc gtaaaaaata ttagatatga
     2881 ttcacgctga ctcataagcc acaaatataa ataacgctta aacaaattat ctgagacatt
     2941 ttaacat
//
LOCUS       DADSZD010000290         2896 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.292, whole genome shotgun sequence.
ACCESSION   DADSZD010000290 DADSZD010000000
VERSION     DADSZD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.292"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(175..1356)
                     /locus_tag="J1Z28_004393"
     CDS             complement(175..1356)
                     /locus_tag="J1Z28_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBA7719146.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLACIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            1611..2840
                     /locus_tag="J1Z28_004394"
     CDS             1611..2840
                     /locus_tag="J1Z28_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBA7719147.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT          752 a          716 c          634 g          794 t
ORIGIN      
        1 taaaacaatg tttcattttc aagttaagga ttaaaaaaag tgcagtgtcg atcacgaata
       61 tgtcatgaga atggaaaaat cagggagccc tgaaaaggta tgtcgactca ccagccatca
      121 ttattcaaca gattaatgac ggcagatatt tatcggtaag gaggaaatgc agagctactc
      181 gtcacgttca atggtcaatg ccacaccctg gcccccgccg atacaaagcg ttgccagtcc
      241 tttgcgggca ttacgtttca ccatttcatg aacaagagaa accaggattc ggcaaccgga
      301 agcgcctatc gggtgaccga gtgcgatcgc accaccattg acattgaccc gacgctcatc
      361 ccattcaagc atcttgccaa ccgaaagcgc ctgtgcagca aacgcttcat tagcctcgat
      421 aagatcgaca tcagccaact gccagccaac acgctccagg caacggcggg tcgcatacac
      481 cggcgcaatt cccatcaatg ccggatccac accaacgctg gcaaatgcgc ggatgcaggc
      541 cagcacgggt aaattcaacg ctcgtgcttt ggcttcgctc atcatcatta ctgcagctgc
      601 cccatcgttt atggatgatg cattacccgc tgtcacagaa ccgagactat caaatgaagg
      661 atttaaacgg gctaagcctt ctgcgctggt gtcagtgcgt ggctgttcat cggtatcaac
      721 aaccaacgtc tgtccgttac tttgggttat taccgggacg atctcatctt taaatcgtcc
      781 ggcgtcaatc gccgctcgcg ctttttgttg cgagctaagt gcgtaagcat cctgcaactg
      841 acggctgatg ccatattcgc gagccagatt ttcggcggtg acaccaatat gataatcatt
      901 gaaggcatcc cacaacccat catgcacaag actatcaacc aactggctat tgccaagctg
      961 tgcgccggtg cggctatcag tcagaacatg tggtgcgcgg ctcatgtttt cctggccacc
     1021 ggcgatgaca atatcagcct cgccacactg tatcgcctga gtagccagat gcagtgcttt
     1081 aagcccggaa ccacaaacgt cattaatagt gattgcagaa acgctattag gaagaccacc
     1141 tttaatagcc gattgtcttg ccggattctg ccctgcccct gcagtcaaca cctgaccaag
     1201 aatcacttca tccaccgcat atgcaggaac gccggtacgt tctattaacg ctttgacgac
     1261 caggctgcca agttccacgg cggaatgacc cgctaacgca ccacgaaagc agccgatagg
     1321 tgtccgtaac gccccgacaa tcacaacgtc tttcatcaca acctcgcacc aattaacagt
     1381 gcaatagtaa atgattgtta attacagtta tcttaattgt ttaaaaaaag tgactattat
     1441 cacaaaggaa atacgcctga gtagtagtca gagacataac tggcacgtaa ggtttgcaac
     1501 cactaactca ccaatagaga ggtagatagg gcgttaatct cccatactta acctggttta
     1561 tggtaaattg ccctccattt tgtttaattt gtagatgata cgttcagata atgtctaata
     1621 tttggtcaaa agaagaaact ctgtggagtt tcgcgctcta cggcacagcc gttggtgcag
     1681 gcacgctctt ccttcctatt cagttaggtt cggcgggggc tgtggtcctg tttattactg
     1741 ctctggtcgc ctggccttta acatattggc cacataaagc cttatgccag ttcatcctct
     1801 catcgaaaac atcagcaggt gaagggataa cgggcgcggt aacacactac tatggcaaga
     1861 agattggtaa tctgattacc acgctgtact tcatcgcctt ttttgtcgtc gtgttgatat
     1921 atgcagtggc aattaccaac tcacttacgg aacagctggc aaagcatatg gttattgatc
     1981 ttcgcatccg tatgttggtg agtctggggg ttgtattaat cctgaatctc atttttctga
     2041 tgggacgcca tgcaaccatt cgggtaatgg gatttttggt attcccattg attgcctatt
     2101 tcttatttct ttctatttac ctggtcggta gttggcaacc tgatctatta acaacccagg
     2161 tagagttcaa tcagaatacc cttcaccaga tatggatatc gattcccgtg atggttttcg
     2221 cttttagcca tacgcccatt atttctacgt ttgccataga cagacgtgaa aaatatggcg
     2281 aacatgctat ggataaatgc aaaaaaatta tgaaagtcgc ttatctcatc atctgcataa
     2341 gtgtattgtt ctttgtcttt agctgcctgc tttctattcc accttcgtat attgaagcgg
     2401 ctaaagaaga aggggttacc attttatcgg cgctttctat gctgccgaac gccccagcat
     2461 ggttatcaat ttccgggatt attgtcgcag tagttgcgat gtcgaaatca ttcctgggta
     2521 cgtactttgg cgttattgaa ggtgccacag aggtcgtcaa aacaacatta cagcaggttg
     2581 gtgtaaagaa aagtcgtgca tttaaccgcg cactatcaat tatgttggta tcgctgatta
     2641 ccttcattgt ttgttgcatt aacccgaacg cgatttcgat gatttacgcg atcagcggcc
     2701 cgctcattgc catgatactt ttcatcatgc ctacgctgtc aacgtatctc atcccggcgc
     2761 ttaaaccctg gcgttccatc ggaaatctga ttacgctgat cgtgggtatc ctgtgcgtat
     2821 cggtaatgtt ctttagctaa tatccgttgt ggatggttca aaattgcttc aattttgaac
     2881 catcccagtt ttcata
//
LOCUS       DADSZD010000291         2876 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.293, whole genome shotgun sequence.
ACCESSION   DADSZD010000291 DADSZD010000000
VERSION     DADSZD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.293"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..110
                     /locus_tag="J1Z28_004395"
     CDS             <1..110
                     /locus_tag="J1Z28_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBA7719148.1"
                     /translation="TERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            173..394
                     /locus_tag="J1Z28_004396"
     CDS             173..394
                     /locus_tag="J1Z28_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA7719149.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            557..925
                     /gene="cbtA"
                     /locus_tag="J1Z28_004397"
     CDS             557..925
                     /gene="cbtA"
                     /locus_tag="J1Z28_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBA7719150.1"
                     /translation="MSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            1015..1392
                     /locus_tag="J1Z28_004398"
     CDS             1015..1392
                     /locus_tag="J1Z28_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBA7719151.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTHSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEAKQ"
     gene            1389..1877
                     /locus_tag="J1Z28_004399"
     CDS             1389..1877
                     /locus_tag="J1Z28_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719152.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDIDGVNLAELINNVAENGYS
                     LRVAEESDQQSTCTPPPFTILAGIRCSTAHITETDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRMVVTLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            1889..2086
                     /locus_tag="J1Z28_004400"
     CDS             1889..2086
                     /locus_tag="J1Z28_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HBA7719153.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            2183..2752
                     /locus_tag="J1Z28_004401"
     CDS             2183..2752
                     /locus_tag="J1Z28_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBA7719154.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     SAHITFKRLELIDRINDIIARHFPSVLSA"
BASE COUNT          735 a          778 c          685 g          678 t
ORIGIN      
        1 tcaccgaacg tctggtacag gcactgggcc tggtggatat ccgggtgccg gaccatctga
       61 tagtcggtgg caaccaggtt ttctcctttg ccgaacatgg tctgctttaa cccgtcacaa
      121 ccacatcaca cctgttttca cttttatctt ctgtcttcag aggtatccca ttatgaaaat
      181 tatcactcgt ggtgaagcca tgcgtattca ccaacaacat ccgacatccc gtcttttccc
      241 gttctgtacc ggcaaatacc gctggcacgg cagcactgaa gcgtataccg gtcgtgaagt
      301 gcaggatatt cccggtgtgc tggccgtgtt tgctgaacgt cgtaaggaca gttttggccc
      361 gtatgtccgg ctgatgagcg tcaccctgaa ctgatgttcc gtcacccgga aaacgagtac
      421 gactggctga gctcagacgc cgctgtggat gaggccctgc tcataaacga atacaccttt
      481 accgaaggcg gtctgcgcgc cggctgacct gtcatcgtta aaccattctt aaccgatttt
      541 ctgtgaggat tttatcgtgt cagacgcact ctccgggaca atgcttcccg acgacaatca
      601 cgaccgcccc tggtgggggc tgccctgcac cgtgacgccc tgtttcgggg cacgtctggt
      661 gcaggagggt aaccgcctgc attaccttgc cgaccgcgcc ggtatcagag gcctgttcag
      721 cgatgcagat gcgtaccacc tggaccaggc ctttccgctg ctgatgaaac aactggaact
      781 catgctcacc agcggtgaac tgaatccccg ccatcagcat accgtcacgc tgtatgcaaa
      841 aggtctgacc tgcaaagctg acactctcgg tagcggtggt tacgtttatc tggctgttta
      901 tccgacgccc gaaacgaaaa agtaaaccac cggaataacc ttctgctccg gcctggtgct
      961 ttcaccacgc cacttttcca tttttcatct ctgcatatca ggaaaacctt cagtatgaaa
     1021 acattacccg atacacacgt acgggaggca tcgagctgcc cgtctcccgt caccatctgg
     1081 cagactctgc tctcccgact actgggccag cattacggcc tcacgctgaa tgacacaccg
     1141 ttcgccgatg aacgcgtgat tgagcagcat attgaggcgg gtatttcact gtgtgacgcg
     1201 gtgaactttc tcgttgaaaa atacgcgctg gtacgtaccg accagccggg attcagcgcc
     1261 tgtacccact ctcagttaat aaacagcatc gatatccttc gggctcgccg ggcaaccggc
     1321 ctgatgaccc gcgacaatta cagaacagta aataacatta ccctgggtaa gcatccggag
     1381 gcgaaacaat gaaactggcc ctgacgctgg aagccgacag tattaacgtg caggcgctga
     1441 acatggggcg cattgtcgtt gacattgatg gtgttaatct cgctgaactg attaacaatg
     1501 tcgctgaaaa cggttattca ctccgcgtgg cggaggaatc cgaccaacag tcaacctgca
     1561 caccaccacc gtttacaatc cttgccggca tacgctgcag taccgcacat atcacggaaa
     1621 cagacaacgc ctggctgtac tcgctgtcgc atcagaccag tgacgttggt gaatcggaat
     1681 ggattcattt cacaggtagc ggatatctgt tacgtaccga tgcgtggtca tacccggttc
     1741 tgcggcttaa gcgcctgggc ctgtcaaaaa cgttccgtcg tatggttgtc acacttaccc
     1801 gacgttatgg cgtcagtctc attcatctgg atgccagcgc tgaatgcctg ccgggtttac
     1861 ccactttcaa ctggtaacca ggaacaacat gaaatcatta accacggaaa cagcactgga
     1921 tattctgatt gcgtggctgc aggacaatat cgactgcgaa tcgggaatta tctttgacaa
     1981 caatgaggat aaaacggatt cagcagcact gttgccctgt atcgaacagg ccagagagga
     2041 tatccgtacc ctgcgccaac tgcagcttca gcaccagaac cggtgagtct cactcatcat
     2101 ctcactcacc agacttcatt ccactgacgc cagcctgaac acggctggcg ttttcattta
     2161 tctgcaaaaa ggaatatcga ttatgtctga aatcacagtc tcccgtccgg aagtggtcaa
     2221 cgagaatacg gacgttatct gctccacctc agtcaggtcc aggtcactgg aatatgataa
     2281 ttttccggaa atcagcgaag cgaacattct gagcacattt gaacaactgc accagaacaa
     2341 agatgaagtg tttgaacggg gagtgatcaa cgtcttcaaa gggctgagct gggattacaa
     2401 aaccaactca ccctgtaaat ttggcagtaa aattatcgtc aacaatctgg tgagatggga
     2461 ccagtgggga tttcatctta tcagtggaat gcaggcagat cgcctggctg acctggaaag
     2521 aatgttgcat ctgctcagcg gtaaaccgat ccccgacaac cgagggaata tcaccattaa
     2581 tctggatgac cacatacagt ccgttcaggg taaaggacgc tatgaagatg agatgttcat
     2641 cattaaatac tttaagaagg gatctgcaca catcactttc aaaaggctgg agctgattga
     2701 cagaattaac gatataatag ccaggcactt tccttctgtg ctctcagcct gaccccgagt
     2761 ttgattccct ttcgatatca aaagggactg cgggtacaaa agagggtaca tatttcacca
     2821 aaccaaacaa aataaactaa tatcaacatg atagaagcat tcttcgattc cgagtc
//
LOCUS       DADSZD010000292         2712 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.294, whole genome shotgun sequence.
ACCESSION   DADSZD010000292 DADSZD010000000
VERSION     DADSZD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.294"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..354
                     /locus_tag="J1Z28_004402"
     CDS             <1..354
                     /locus_tag="J1Z28_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA7719155.1"
                     /translation="KRRKDKSVEPTPLYYLWSREAVANDTTPYLMVLLLNNDVWCKSR
                     EVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFNAANTYNAESGVLEQTARAYI
                     ESHGAIERHLGCSDF"
     gene            674..1870
                     /locus_tag="J1Z28_004403"
     CDS             674..1870
                     /locus_tag="J1Z28_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBA7719156.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLDHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKR
                     LAADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRGEVALEVEQRVLAHPLYP
                     VLTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRAEHPSRRGN
                     KALKRALFLSAFAALRDPLSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTF
                     YTPQAS"
     gene            1941..2174
                     /locus_tag="J1Z28_004404"
                     /pseudo
     CDS             1941..2174
                     /locus_tag="J1Z28_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015841669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter solute-binding protein"
BASE COUNT          645 a          723 c          694 g          650 t
ORIGIN      
        1 aagagacgta aagataaatc tgttgagccc acccctttat attatctctg gagtagggaa
       61 gctgtggcta acgataccac tccttatctt atggtgctgc tacttaataa cgatgtctgg
      121 tgtaagagtc gtgaggtggt ggttctcaga aatctgattg agcaggcgtg gggaaaagta
      181 ttgggtgaag ataactccat tacacatctg gttgtgtatc ctgaagtgtt gtcgttggta
      241 tttaatgcag ctaacacata caacgctgag tctggtgtac tggaacagac ggcccgggct
      301 tacattgaat cgcatggtgc cattgaacgt catcttgggt gtagtgactt ttaacgtcag
      361 gtgatgcata agggtctttc ggggagaggc cctgtttaca caattaaata agtcgagtcc
      421 tccaggctgg gctgtgtgca cgccccgtta ctttattcgt accccttata atggggtgtt
      481 agccagccat acccggcatg attactgccc ccagtcgtcc atgatccggg gggtgatgtc
      541 accgggtctg gtggggcgct ggtaaccgct aataggggtc aggtcaggca cttttgccgg
      601 gaccgtctgt aacgtggatg ccggtacctg ctccccgtgg ttatctggtt aacccatata
      661 caagggagac agaatgaccg aatccagcga ttacgaatcc gtccaggtct ttatcggcgt
      721 tgatgtcggt aaagatacgc atcacgctgt agccattaat cgttcaggta aacgcctgtt
      781 cgataaagca ttacccaacg acgaaaacaa actcaggtcg ctaatatctg acctgaaaca
      841 acatggtcag atactgctgg ttgttgatca gccagctacc atcggtgcgt tacctgtcgc
      901 cgttgcccgc tcagaaggag tccttgtcgg atacctccct ggactggcca tgcgccgcat
      961 agccgactta cacgccggtg aagctaaaac tgatgctcgt gacgctgcca tcattgccga
     1021 agctgcccgt accctgcctc acgcgctacg cacgctgaaa ctggctgacg agcaaatcgc
     1081 cgaactctcc atgctctgcg gcttcgatga tgatcttgcc gcacagacaa cgcaggccag
     1141 caaccgtatc cgcggccttc tgacccagat acatccggca ctggagcgcg ttctcggtcc
     1201 gagacttgat cacccggcgg tactcgatct tctccagcga tatccctcac cagaaaaact
     1261 cgcttcgctg ggtgagaaga agctggcagc ccagctctgc aaacttgcgc ctcgtctggg
     1321 taaacgcctt gcagcagaca tagctcaggc actggccgaa caaaccgtcg tcgttcccgg
     1381 cacgaatgcc gctgccgtag tactgccacg tctggcactc cagctcatca cgctgcgtaa
     1441 gcaaagaggc gaggtggcgc ttgaggtaga acagcgagtt cttgctcacc ctctttaccc
     1501 ggtcctgacc agtatgcccg gagtcggtgt caggaccgca gccagactcc tcaccgaggt
     1561 cgcctgccgc gccttcgcct ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt
     1621 aactcggcga tccggctcgt caatacgcgc tgagcatccc tcgcgacggg gtaataaagc
     1681 tctcaaacgg gcgttgttcc tgtcggcctt cgccgcgctc agggatccgc tctccagggc
     1741 ttactacacc cgcaaaatga gtcagggaaa acgacacaat caggcgctta tcgccctggc
     1801 gagacgacgc tgcgacgttc tgttcgccat gatgcgcgac gggacttttt ataccccgca
     1861 ggcgtcataa catgcttgac aacttaatag gggcacgtta ccaatactgg cttaatggct
     1921 acatacggcg gtcagtttac gcttacaaaa atgtggcgaa actgattaat caaattaaga
     1981 gagagcacgt aaaattgtac tttatcgaaa accagaccga tccccgtttg gtgaaacaaa
     2041 tcgccaatgc cagtggtgca caagcgggag gagagcttta tccggaagca cttaccgaca
     2101 gtagcggcct cgctgctacc tacacggcag cgttcaaaca caatgtcgat accctcgctg
     2161 ctggcatgaa gtaagagagt aggcaaaaaa gtaagtagat atcaggttgc cccatatcat
     2221 caggtaacct gtgacacttc tcttgccaga gcgccatgtt ccagcgcctc actcactatc
     2281 tgcttatcat ccgactttcc ctcatacctg tctcgattat aaagataaaa cgatgatgcc
     2341 cgggttcatt gtgcgaaggc atggcatatt tgttcccggt atcgtcagac agcatcattc
     2401 gactctccat ctgctgtctg gccagacaaa agatggcctt gtttgccgcg gtggaaatgg
     2461 agggaggacg agcccacaag catgaaaaac ttaacccagg ctgtggaagt gcaacttttg
     2521 ctccggttgc tgcagtaaaa cacgagtata cgtctgccgt acacattcat aagcgattcc
     2581 gatggctcac ttacgtggag aaatggagtg ccgtctgttc tttatttatt gtttgcgtta
     2641 tgttataaca tataaaagag tattgtttgg catctgtaac ttattgagaa ggcaaactgc
     2701 agagtggtta at
//
LOCUS       DADSZD010000293         2704 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.295, whole genome shotgun sequence.
ACCESSION   DADSZD010000293 DADSZD010000000
VERSION     DADSZD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.295"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(46..2703)
                     /gene="chiA"
                     /locus_tag="J1Z28_004405"
     CDS             complement(46..2703)
                     /gene="chiA"
                     /locus_tag="J1Z28_004405"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HBA7719157.1"
                     /translation="MCSALPALAMEAWNNQQGGNKYQVIFDGKIYENAWWVSSTNCPG
                     KAKANDATNPWRLKRTATAAEISQFGNILSCEKSGSSSSSTSNTPASNTPANGGSATP
                     AQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNCPGDAKGNDASNPWRY
                     VRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPAPADKSNDNYAVVAWK
                     GQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRYVRAATATEITQYGNP
                     GSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADSSNDYSLQAWSGQEGS
                     EIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTATAAEMSQYGNPTTCEI
                     DNGGVIIADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVPGGDNPWKKYEPAKAW
                     SASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNSRPWKPLGKTQSYSNE
                     ELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNPWRVFVDWTGTKERVG
                     TPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVVSKDANTCLPTWGTAY
                     GMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDLMQHYYDIVDNLNLRV
                     LDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTLPILPTGLTPEGMNVL
                     SDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSAIANLHAQLKGLYGNK
                     SDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLGMVGIWSIARDLPGGT
                     NLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          627 a          718 c          646 g          713 t
ORIGIN      
        1 cattgacaaa aaatgcggcg ataccggaag gtatcgccaa cacatttatt gcttagtaaa
       61 cggcgcgaag atttcgctaa atgcgtactt cggagcctgt tctttagtca gaccgtggaa
      121 ttccggagac aggttagtgc cgcccggcag gtcgcgcgcg attgaccaga taccaaccat
      181 accgagatta cgcttctgcg catcctgcat gaccagacga gcatcagaga gatagaacac
      241 ctcaccctga acgtcgttca cgccaaccat cggcgtggta cccatcatgg cgtcaatttc
      301 tgcatcactc ttattgccat agaggccttt cagctgcgca tgcaggttgg caatcgcaga
      361 cgttgcacac ttaccgtgga tgttctggcc ttcggtgttt gcagactgac aaatagcgtt
      421 accgtagtcc attgtcatca cgttcacacc cgccagctca acacctttcg ctttggcatc
      481 gctcaggaca ttcatccctt ccggcgtcag gcctgtcggc agaattggca aggtatacca
      541 gatagcaata tctttgcctt ctgacttcca tttatcctgc actttcttca cagcaaggtt
      601 acgacgttca atagatgcct gatccgcaac ccaggtgcct tcgatatcga agtccaggac
      661 tctgaggttc aggttatcaa cgatgtcata ataatgctgc attagatcgt caacgttctt
      721 acaggaagct gccagcggag cgttgttagc accaccgata gacagcatca catcaccgcc
      781 agcctcacgc agcgctttga ttttgctgta ctgagcgtaa ttctgcatac cgtaagcggt
      841 accccatgtc ggcagacagg tgttcgcatc tttactcacc acaaacgcca gcgtgaagtg
      901 gttgacgtta tgattcttag ccagcgcagc caaatccggg atcgtattca gcgtaaagtc
      961 gacatacggt gcataaacgt gtttcggcca cgctttcttc ggcgtaccta cgcgctcttt
     1021 ggttccggtc cagtcaacaa aaacacgcca cgggttgctg tcagaaggag aaactttctg
     1081 cactttactc tgagaaatgt agttctcacc gttaaagcga atcagcgtat cactggcata
     1141 aagcgtttct ggattaaact gcggcgcatt attcagctct tcattgctat agctctgagt
     1201 cttacctaac ggcttccacg ggcgactatt gctaccggtg gcgttttggt tcgcaaccag
     1261 agcagggttg tcactttgcg tccagaacag cgcttcataa gcctgcccat caacaacaac
     1321 gcgatcacct ttcacgtaca cggtggaagc ggaccatgct ttcgccggtt cgtatttctt
     1381 ccacgggtta tcgccgccgg ggacaaagcc ccatgcgtct tgatcgacag aggttttata
     1441 atgtgcatcg ttataatcta cgatgctgtt cgcgctgtac gctttgctgg cctggaaacc
     1501 atccgcaata atgacgccgc cattatcaat ttcacaggta gtcgggttac cgtactgact
     1561 catttccgca gcggtagctg tacgcacgag gcgccatggg ttattggagt tttctacact
     1621 ggtgccacgc gggcaatctt cagaaccaac ccaccaggca ttctgataaa cattaccatt
     1681 gaagataaca tggtaaattt cgctgccttc ctggccactc cacgcctgca gtgagtaatc
     1741 gttactgcta tccgctggtg ttgatggctc gttgttatcc ggagttggag taactggagt
     1801 ttccggtgtt tccggagttg gatcaaccgg agtcacagca ccgccattat tatccggctt
     1861 aacggaacag gagcctgggt taccatactg agtgatttct gttgccgttg ccgcgcgaac
     1921 ataacgccat gggttgctgg catcgttttc tttcgcatcg cctgggcaat ttgccgcgcc
     1981 tacccaccag gcgttcttat aaatgctgcc gttgtagata acgtaccatg tagaagaacc
     2041 ttcctgacct ttccaggcta cgacagcata gttgtcgttt gacttatcag ctggggctgg
     2101 ctgaacatca ggagccggtt taacgtccgg tgccggtttc acatccggtg caggctgtgg
     2161 tgctgaagta caagactgtg gattactggt ttctgagatt tccgttgccg tagcggcacg
     2221 aacataacgc catgggttgc tggcatcatt gcctttcgca tcacccgggc agttagaaga
     2281 ggctacccac caggcatttt tatataccgc accattaaag acgacatacc aggtttgacc
     2341 gccctgctgt ttattccagg caactacaga agaattagac ggaacagtgc cctgtgctgg
     2401 tgtagccgaa ccgccattag caggcgtatt agatgctggc gtatttgaag ttgaagaaga
     2461 tgagcttccg ctcttttcgc aggaaagtat attgccaaac tgactaattt cagcagctgt
     2521 tgcggtgcgc tttaaacgcc acgggttagt tgcatcattt gctttcgctt ttcccgggca
     2581 atttgtagaa gaaacccacc aggcattttc ataaattttg ccatcgaaaa taacctgata
     2641 tttattacca ccttgttggt tattccatgc ttccattgcc aatgctggca gagcggaaca
     2701 cacc
//
LOCUS       DADSZD010000294         2685 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.296, whole genome shotgun sequence.
ACCESSION   DADSZD010000294 DADSZD010000000
VERSION     DADSZD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.296"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..150)
                     /locus_tag="J1Z28_004406"
     CDS             complement(<1..150)
                     /locus_tag="J1Z28_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7719158.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQIQ
                     MATYEH"
     gene            complement(166..2646)
                     /locus_tag="J1Z28_004407"
     CDS             complement(166..2646)
                     /locus_tag="J1Z28_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBA7719159.1"
                     /translation="MLRMTPLASAIVALLIGIEAYAAEETFDTHFMIGGMKDQQVSNI
                     RLEDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREMIKRLGINTDSFASGK
                     QCLTFKQLIQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLQEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGHQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNLRRISYTLAASHAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSATFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQYQGNETTAGANL
                     TWNAPVATVNGSYSQSSAYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENYLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESKNYICQ"
BASE COUNT          748 a          696 c          497 g          744 t
ORIGIN      
        1 atgctcatat gtcgccattt gtatttgagt aagtgcaaaa tccccctgaa caaccaaatt
       61 gtgctgaaca gataactcac tataacatgc cgcatagctc acaggcataa ccatcatcat
      121 tactataaat agcattatgc gaatttccat agaaccacct gactattact ggcaaatata
      181 atttttactt tcatcaatct ctttaccgaa ggtgattgtg catgaaagcc cttgttgctt
      241 atcaattgcc acattaaccg atggcggtat ttcattggtg cgaataaata actggcttcc
      301 ctggccgaca acaccaatat tatgaccatg gatatcattg acttcataac caaacattaa
      361 tggttgccca tccgctctta atgcttttat aaaccatggc ttgcgctgat cggtatcaaa
      421 attaaccagt acaaccgcgc cacgataagg ggcggcaatt ttccggttgc cacgtaattc
      481 tgcttcgcta tcgctttgtg acacatccaa catcagataa ttttcccgat aaggtgtcat
      541 tccgtcgtat accaccactc cattacggtt tgttgtgcga tatttttgcc cattgacata
      601 agcatcttta attcctggcg cattcatcac agcaaacgtt tcagaaagac ggtttgccag
      661 attaacgcca cccgaccagg cgacaatgcc ccctgaaaca ctggctccag cctgtcgata
      721 agcactcgac tgactataac tgccattcac tgtcgcaacc ggcgcgttcc aggttaaatt
      781 cgccccagct gtcgtttcat ttccctgata ctgataactc aggttgaccc catagttaaa
      841 ctgatcccgg cttccaacgg ttcctgataa tcccgtatta tttgaggcaa acccctgatc
      901 atcaaacgtc gctgagttag acatatatat ttgccgacga ggcgtcgtaa cgtcatcacc
      961 ccaatcaaag ggaatcgata taaaaatatt aaaacgtttc tcttcatgat gattctcgtc
     1021 ataagcatgg cttgccgcga gggtatagct tatccttcgc aggttgttgg aataactcaa
     1081 ctgataatcc ttactgctgc cgctacgccc ccagtaatct cgccataacg tacttaagga
     1141 cacagaaccc cagccttctg gcaatgactg actcatattg gcagagaagc tatttttacg
     1201 gccaaaatcg ttctggtaat aatcggcaat gtcatagata tcgttttcat cacggcgata
     1261 attatcttta ttgtttgccc atacgtgatc attaaatgtc cgataatcac gcgacgaata
     1321 acgccaggcc gccagaccaa aacgtgtcga cgtttggctc acaaatttgt tgtaggcaat
     1381 ttgataactt tgcccgtcaa acacatcgcc gttgtcttgt ttactatgcg acttcgtggc
     1441 atcgacggaa atggcaccaa tgcgtgtatt ccagccagtc cccaaagtga acgcgtaata
     1501 attattcgcg accatcgagc caccatacag cgttaataag ttattaaaac catactgatg
     1561 acccgcctgg acaaaatcac tttgtttgct cgccccttca atatggctac gacccgccgc
     1621 aaaatcatat ttcgacacgc cgggttgcag catatttggt accgctgcat aaggcaccag
     1681 ataggtggtt accgaaccgt ccgcctcttt cacgctgaca tcaagatccg ctccaccacc
     1741 ggccaactgc aaatctgtaa tcgcaaacgg gccaggagga acctctttct gataaaccac
     1801 aaaaccattc tgttcaatag ttaccagcgc gttactctga gcaatcccct gcacccgtgg
     1861 cgtaaaattt tgtttcgagt taggcaacat ctgcatatca cgaaacaacc gcacaccgct
     1921 gaagcgaaca gaatcaaaaa tatcgcttga tgtgtacata tcacccacac gaagcgtgcc
     1981 gagaagttgg gcaaatccac gttccagata cagggtattg cttttccaca cccctggatt
     2041 gttatttgtt ttactgaaac tggcatcaga atgcagttgc cactcctgta aatttaaccc
     2101 gctgttaaaa cgtacatatg tactcttgct attaccggac gctttataat cgctgtaata
     2161 ctgactcaca taataagagg tataaaacgc attaataccc cgctcccagt tttctggtgg
     2221 aacatagcca ctttccagct cttccaccca ggcctgcggg acactgaaat cgagacgaaa
     2281 aactccgata tcccaggtat agctcccgcc ctgaataagt tgcttaaatg ttaaacattg
     2341 cttaccgctg gcaaagctat cggtattaat gcctaaccgc ttgatcattt cccttgataa
     2401 acatgtttct tgcgggttgt ctttaacaat gatctcatat ttcccacgcc attgcttatt
     2461 gacataaata tcgatgtcat actgccccgg caagggctga ttatcttcaa gacgaatatt
     2521 tgaaacctgc tggtctttca ttccacctat catgaaatgg gtatcaaagg tttcttcagc
     2581 tgcataagct tcaatgccga tcaataacgc tacgattgct gatgcaagtg gggtcattct
     2641 caacataggc agctcctgca attaaatttt gtcactaata taatt
//
LOCUS       DADSZD010000295         2650 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.297, whole genome shotgun sequence.
ACCESSION   DADSZD010000295 DADSZD010000000
VERSION     DADSZD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.297"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(41..1276)
                     /gene="preA"
                     /locus_tag="J1Z28_004408"
     CDS             complement(41..1276)
                     /gene="preA"
                     /locus_tag="J1Z28_004408"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBA7719160.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELHDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(1270..2508)
                     /locus_tag="J1Z28_004409"
     CDS             complement(1270..2508)
                     /locus_tag="J1Z28_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBA7719161.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYLPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
BASE COUNT          648 a          722 c          610 g          670 t
ORIGIN      
        1 atccggcgac aggaactaaa tgccggatgc gtcgctggtt ttacaacgtt accgggtgtt
       61 ctttctcgcc tttcttaaac ttcacttccc caagatcaat acaacctacc gggcagacat
      121 gaccacaaag cagacaaccc acgcatttct cggtattaca atgcggtgtg cgggttttct
      181 cgctccattc catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc
      241 caacacattt atcaagattg atatggggat agacaatata actgcggtct aaatcttccg
      301 ccgggacaat attgtggttc gccagaccca ccatctcctg caacgaatca aacccttgat
      361 cggcgagata atgcgacaac ccgcttgcca tatcttccac tatccgatac ccgtactgca
      421 taatgccggt ggtcacctgc aacgttgctg cgccgagcaa taaaaactcc gccgcatctt
      481 cccaggtttc aatgccgccg ataccgctga tcgggaaatc gtgcagttct ggatgggtgc
      541 gcatttgctg aataaagcgt agggcgatcg gttttaccgc tttaccggaa tatccggaaa
      601 tactcgattt accgttaacg atcggcatac caattttctg attaagatcg atattggtga
      661 tggatttaac ggtgttaatc gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg
      721 ccacttcgca catatcacca atattcggcg tcatcttcgc cagcattggc agcgtggaac
      781 cacgttttac tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac
      841 ccatcgcatg agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag
      901 cttcttgcac cagacgcgcc agctcttccc attgctgctc attttcgccc atgattgaag
      961 cgatcaatac tttgtccggg taatcttctt tcagccgacg cagggcggcc agattctctt
     1021 ccaacggatg ttcagcaatc tgctccatat ttttgaagcc gataaaaccg gtatcttctt
     1081 tcaccagatg atcaaaacgc ggcgagactt cgttggcgat aaaaaaaccg atcgttttaa
     1141 acaccacgcc gccccaacct gtgtcgtagg ctttggcaca catctcatag cagttgccta
     1201 ccggcgaaga agaaaggcag aacgggttgg gaaacttcac gccgcaaaaa gtaatcgaaa
     1261 gatctttcgt taacatgagc aagctccctc taaatagtga tgaatcgccc cggcggcttc
     1321 tttcccggtt ttcactgcat agaccacggt tttgtcaccc tcaacaatat cgccagcagc
     1381 aaagacttgc gggtcgcggg tctggtaatg ttgcgtttcg atggtgttac gctgcggctc
     1441 taactccgca aaggcatcca gtctggcatg ctgaccgacg gcgagaataa ttttatctgc
     1501 cgccatcgtc agttcgcccg gtaaacgaac atgcttaaag gtgactttat tgccttccac
     1561 ggctactggt gtaaatccat cgatgatcga aacgcccagc tctctggcgc tggtaaactc
     1621 tttttcgctt gccggaaact catctaactc ttcacgcgct acgcaggtta ccgcctgaca
     1681 gccgagaact ttcagcgtgc tggctacgtc catcgcaaca tcaccgccgc cgataattaa
     1741 tgcgctttgt ggaatgctga tatcgccttg cgcctgtcgt gcacgttgca ggaagtcgac
     1801 ggcaatctca acgtcgctat gctcaaacag cgataacccg gaaccgctcg ataaccctac
     1861 ggtgaccagt accgcgcggt tttccgcttt cagctgctcc agggtgagtg ttttaccgat
     1921 ttcgttattg cacttaatgg tcacgcccat tttttctata cgggcgattt ctgcatccag
     1981 caccgattgc ggtaaccgga attgcggaat gccgttacgc aaccagccac cgggttgtgc
     2041 ttctttctca tagatcgtga cgtcataacc ctggtttgtc agcgtcacac tggcctgcaa
     2101 tccggcagga cctgcgccaa taatggcgac tttgccgagc gttttagtac cgggcagata
     2161 aatttccatc ccagtttgtt gttcaaaatc agtaacaaaa cgctgtaagc gaccgatatc
     2221 aatgggagca tcaacaccgg cacgtgtaca accgctttgg cataatttct ccgtcgggca
     2281 cactctggca caaacggcac cgagggcatt attttcgcga attgtctcgg cagcgccttt
     2341 aaaattacga aagtagattg agcgaataaa tttccccgga tcggtctgtg ccgggcaagc
     2401 ctgactacag ggagcgtcat gacataataa acagcgagag gcttctttaa tcgccagtaa
     2461 aggcgtaaaa gccggagtga gttcatccag ataattttgt tgcggcatgt gatcttcctt
     2521 aatgctatga gtaattaagg cgaacgctaa ataaatattg cgtgctgata ttaaggctat
     2581 gcgcgtgggg aaatttaaac tgtgaaagga ttcacaaata gctgcggttc agaatgttat
     2641 atccgcagct
//
LOCUS       DADSZD010000296         2632 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.298, whole genome shotgun sequence.
ACCESSION   DADSZD010000296 DADSZD010000000
VERSION     DADSZD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.298"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            23..1075
                     /locus_tag="J1Z28_004410"
     CDS             23..1075
                     /locus_tag="J1Z28_004410"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBA7719162.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1151..2584
                     /locus_tag="J1Z28_004411"
     CDS             1151..2584
                     /locus_tag="J1Z28_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBA7719163.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSDYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT          623 a          592 c          678 g          739 t
ORIGIN      
        1 tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc gaggtggaac
       61 ctcgaggggc gcgttcctgt tagcggaaca tctacccgaa gatcaaacgc agcgcgataa
      121 aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta ttggcggcgg
      181 taatccgctg accagtaaag tcgccattat tagccgctcc agcgatccgc gtgcagatgt
      241 cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca cgcctaactg
      301 cggcaatatg ctgtctggcg ttggggcatt cgccattgaa aatggtttga ttgcagcgac
      361 ttcgccagtt acccgcgtac gtatccgcaa cgtcaatacg ggtacgttca tcgaagctga
      421 tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg acggcgtacc
      481 gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gccggaacga aaaccggaaa
      541 agtcttcccg actgataatc agattgatta ttttgacgat gtcccggtga cctgtatcga
      601 tatggcgatg ccagttgtca ttattcctgc tgaatatctg ggaaaaacag gctatgaatt
      661 accggcagag ctggatgccg acaaagcatt attagcccgc attgaatcta tccgtctaca
      721 agcgggtaaa gcaatgggct taggcgatgt cagtaatatg gttatcccta aacctgtact
      781 tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc cgcattcttg
      841 ccatcgcgct ttggcgataa ccggtgctat tgctatttcc agtagttgtg cattggaagg
      901 caccgtcacc cgacaaatcg tcccttctgt aggatacggc aatatcaata ttgaacaccc
      961 cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga cgttacgcgc
     1021 atctgttatt cggacgacaa gaaaaatatt ttccggtgaa gtttatcttc cctgaaaaat
     1081 tttgttgtcg tgataatgat actcaataaa ggaattctgt atgagtcata cagaaagaac
     1141 aggatattaa atgaataaaa aatcgttatg gaagctaatt ctgatattag cgatcccatg
     1201 tattattggc tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
     1261 tatttacctg gcggccattg tggggctggt tatcaagcct ttcccggaac ctgtcgtact
     1321 gttaattgcc gtcgccgcat cgatggtagt ggtgggtaac ttatcaggtg gggaatttaa
     1381 aaccaccgcc gtattaagcg attactcttc cggtaccacc tggctggtgt tttctgcgtt
     1441 tactttaagc gccgcgtttg taaccacagg cttaggtaaa cgtattgcct atctgctgat
     1501 tggtaaaatt ggtagcacta ccctgggtct gggttacgtt acggtatttc tcgatctggt
     1561 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcatcgtgt taccgatcat
     1621 caacagcgtg gcagtggctt tgggatcaga accggaaaaa agtccgcgtc gtgttggaca
     1681 ttacctgatg atgtccattt acatggtcac taaaaccacc agctatatgt tctttaccgc
     1741 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
     1801 ctggggtgga tgggcgctag ccgccggatt gccgggcatc attatgctgc tggtcacccc
     1861 gctggtgatt tacaccatgt atccaccaga aattaagaag gtggataaca aaaccatcgc
     1921 taaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
     1981 ctttgtactg gcgctgctgg gctggatttt cagtaagtct ctgggggttg atgaatccac
     2041 cgtggcaatc gttgttatgg caactatgct gctgctgggc atcgttacct gggaagacgt
     2101 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
     2161 ctccttatta tcgaaagtta aattcttcga atggttagct gaagtcttta aaaataacct
     2221 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
     2281 gcgttatttc ttcgcttctg gtagtgccta tatcgttgcc atgttaccgg tatttgccat
     2341 gctggcgaac gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctctaa
     2401 ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca tctttggcgt
     2461 gggttacaac gatattaaat cctggtggtt ggtcggtgcg gtactgacga tattaacctt
     2521 cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct ggaacatgct
     2581 gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc tc
//
LOCUS       DADSZD010000297         2621 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.299, whole genome shotgun sequence.
ACCESSION   DADSZD010000297 DADSZD010000000
VERSION     DADSZD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.299"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(401..1933)
                     /locus_tag="J1Z28_004412"
     CDS             complement(401..1933)
                     /locus_tag="J1Z28_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004390424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBA7719164.1"
                     /translation="MSYLPPALPMVNSAIPFPVDALPLIMQNAINYLQDGGKVPTVLA
                     VNAVLAAVSLACHSHINVLNPYTDIEEHCSLNILTLADSGTGKSSVSKQVMKPFDAFR
                     AELAESHRVNQAVWREDYVVWKTSLKALESKLRDTVKKDLCTDEAQAALKSHASIEPL
                     KPLLPTLVYNDTSLAALIAGLNAYPCAGLISDEASNFFDARLKDNLAFFNKAWDGDVY
                     EHNRHQRATQCFKPTLTVSLMLQPSLFFDYMKKDGDKALESGFLSRFLFSNITPNNTL
                     SSCVFSYHYRPDTAYRDESALTCFHDLIGKLLGQQLKQINSGEGKKKILKLSPEAEAH
                     WENIRENWLTYTLPGCAWSYIKPMVLKASTNALRIAALLNYFTNQEDDIITLDAISRA
                     SAMMNWYLNHTAAWFYQFTDEYKFQQDVQVLTQWIQYKFRSTNGLPFKKNDVIKYGPN
                     KFRRSERLEPLLNAIMNTGAFTYIVKSPENPAIYITWLMNDGRYMPFYDDKQPPSPQN
                     PA"
     gene            complement(2081..2293)
                     /locus_tag="J1Z28_004413"
     CDS             complement(2081..2293)
                     /locus_tag="J1Z28_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBA7719165.1"
                     /translation="MTISTVKVLRLPAVVSKLGIARSTIYDWLNAKSPRHDPAFPKPY
                     PLGKQSVGWLESELDEWILQRKQALL"
     gene            2422..>2621
                     /locus_tag="J1Z28_004414"
     CDS             2422..>2621
                     /locus_tag="J1Z28_004414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719166.1"
                     /translation="MKPNDIELLFKEMRGVYENQLNGLKAMRPSIKMLEQYNSEAQQW
                     LDNLRARFENEKDLCLSECESL"
BASE COUNT          712 a          557 c          647 g          705 t
ORIGIN      
        1 aagataattc tgacgagcac agaaattttg gttgcagcca gtcctgaatt attaagaggc
       61 ccaatcaaaa ccacaatcag aaacgactgt agcagcgttg tagcgacaaa ggcgataaca
      121 cgatgcaaca tttctgagct gtagtgctac atcgccagag tgtagtgaag tgaatccgcc
      181 tgttcttggt tgaacgattt gtgagcaaaa gagcatattt catttgcggg tgctttccag
      241 aatgccgcta cagttcgcta cagtagcggt tgtagcagag gagcaatgta tcactgtgta
      301 ttttttcaga gggataaggc tcgcgatctc ctccggacca cgagcctgtg tattgttatg
      361 ttatccaaaa tagtgctcag gtatgaggta gctattctgg ctaagcaggg ttctgaggcg
      421 aaggcggctg tttatcatca taaaatggca tatagcgacc atcgttcatg agccaggtaa
      481 tataaatcgc cgggttctca ggagatttga caatataagt gaaagcacct gtgttcatga
      541 tggcattcag caacggttcc agtctttcgc tgcggcgaaa tttgttcgga ccatatttaa
      601 ttacatcgtt ctttttgaac ggtaagccgt tagtggacct gaatttatat tggatccact
      661 gggtcagcac ctgtacatcc tgctgaaatt tatactcatc cgtgaactga taaaaccagg
      721 ccgcggtatg gttgagatac cagttcatca tcgctgatgc ccgcgaaata gcatcaagag
      781 taataatgtc gtcctcctgg ttggtgaagt aattcagcag cgcagcgata cgtaacgcat
      841 tcgtactggc tttcaatacc atgggtttga tatacgacca ggcacaaccc gggagtgtgt
      901 atgtcagcca gttctcacgt atattctccc agtgtgcttc agcttcaggt gaaagcttga
      961 gaatcttctt cttaccttca cctgaattaa tttgtttaag ctgctgcccg agcaatttac
     1021 caataaggtc gtgaaagcag gttaaagcac tttcatcacg gtatgcggta tccggacgat
     1081 aatggtagct aaacacacag gaacttagcg tgttgttcgg ggtgatattt gaaaagagga
     1141 agcgggataa aaaaccgctc tctaatgctt tatcgccatc ctttttcatg taatcgaaaa
     1201 aaagcgaagg ctgtaacatc aaggacaccg tcagcgtcgg tttaaagcac tgggtggcac
     1261 gctgatgacg attatgctca tacacatcgc catcccaggc tttattgaag aacgccagat
     1321 tatctttaag acgtgcgtca aagaagttgc tggcttcatc agaaatcaga cctgcgcagg
     1381 gataggcatt caggccagcg atgagcgcgg caagcgaggt gtcattatag accagcgtgg
     1441 gtagtaaggg cttgaggggt tcaatcgatg catgagattt cagggctgcc tgggcctcgt
     1501 ctgtgcacag gtctttttta actgtatcgc gcaacttact ttccagtgcc tttaaactgg
     1561 ttttccatac gacataatct tcacgccaga cagcctggtt aacccgatgt gattcagcca
     1621 actcagccct gaaagcatca aagggcttca ttacctgttt gctgacggac gatttgcccg
     1681 tccctgaatc agcgagcgtc aggatgttga gtgaacaatg ctcctctatg tccgtgtaag
     1741 gattgagtac attgatatgc gaatggcagg ccaatgatac tgccgctaaa acagcgttca
     1801 cggcaagtac tgtcggtact tttccaccat cctgaagata gttgatggcg ttttgcatta
     1861 tcaatggcaa tgcatcaacg gggaaaggaa tggcagaatt aaccattgga agtgcagggg
     1921 gtaaatatga catttcaatt tccttttttt caatcccaat aacgcaggaa tgacatcacc
     1981 gatgcggctt accctgccgg gtaagggaat gaggtgaatg ttatttaatg tggtgtttca
     2041 gaatgctcct tcgacagtcg cctgttgaag agagaggaga tcagagtagt gcctgtttgc
     2101 gctgcaatat ccattcatcc agctcggact caagccagcc aacagactgt ttgcccagcg
     2161 gataaggctt cgggaacgcc ggatcgtggc gcggtgattt ggcattaagc cagtcgtaga
     2221 tggtggaccg ggcgatcccc agtttgctga caactgcggg taaacgtagt acttttactg
     2281 ttgatatggt cattttgctt tgcctctgat tagctgtata cggacagggc aaaggctaaa
     2341 gtaagacggc atgtttatga atacgcctac ttgccccccc aaaattactt ttagctcatg
     2401 taactcatcc ttggataaga tatgaaacca aacgatatcg aactgctgtt taaagaaatg
     2461 cgtggggtct atgaaaatca actaaatggt cttaaggcaa tgagaccgtc tatcaaaatg
     2521 ttggaacagt acaacagtga agctcagcag tggttagata acctgagggc acgttttgaa
     2581 aatgagaaag acctttgtct tagtgaatgt gaaagcttaa t
//
LOCUS       DADSZD010000298         2603 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.300, whole genome shotgun sequence.
ACCESSION   DADSZD010000298 DADSZD010000000
VERSION     DADSZD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.300"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            126..2405
                     /gene="ydeP"
                     /locus_tag="J1Z28_004415"
     CDS             126..2405
                     /gene="ydeP"
                     /locus_tag="J1Z28_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBA7719167.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLCERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
BASE COUNT          694 a          615 c          641 g          653 t
ORIGIN      
        1 agtaaaaata gtaagagagt agtcccctac tcttatatat ccatgttgac gataaaccct
       61 atgtatatac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
      121 agcagatgaa gaaaaaaatt gaatcctacc agggagctgc cggtggttgg ggtgctgtta
      181 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccaggatgtt attgccatgt
      241 ttgacatgaa taagccagag ggctttgact gtccgggatg tgcatggcca gatcctaagc
      301 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
      361 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
      421 gagatcacga gcttgaggct gcgggacgtc tcactcagcc tttgaaatat gatgacgtca
      481 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gctcgccttc
      541 aaagctatag tgatcccaat caggttgaat tctatacttc ggggcgcact tccaatgaag
      601 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
      661 ccaacatgtg ccatgaaccg accagtgtgg gattggcagc gagtatcggt gtgggtaaag
      721 ggaccgtgtt gctggaagac tttgagaaat gcgatttagt catttgcatt gggcataacc
      781 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
      841 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
      901 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tataacgtgc
      961 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     1021 atgctgcaag cgccgcaggt cggccctcat tactggatga cgaatttatt caaacgcata
     1081 ccgtcggctt tgacgagcta cgccgtgacg tgctcaattc cgagtggaaa gatatcgaac
     1141 gtatttctgg actaagtcag acacaaatcg ccgaacttgc tgatgcatat gctgctgccg
     1201 aacgaactat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaacg
     1261 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     1321 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     1381 aaccgtctgc agagtttctg gatcgcctgt gtgagcgcta tggcttcacc ccacctcatg
     1441 cacctggaca tgctgcaatc gccagcatgc aagctatatg tacggggcaa gctcgagcat
     1501 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     1561 cgttaacgca attagatctg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     1621 tgaccgcacg gcatagctat attctgccgg ttctgggacg tagcgagatt gacatgcaaa
     1681 aaagcggtgc gcaggcggta accgttgaag attcaatgtc gatgattcat gcctcgcgtg
     1741 gtgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     1801 cgcaagcagc actaccccag agcgtggtag cctgggagta tctggtggaa gattatgatc
     1861 gcattcgcaa tgacattgaa gctgtgcttc cagagtttgc cgactataac cagcgcatcc
     1921 gtcatcctgg tggttttcac ctgataaacg cagcagctga aaggcgctgg atgacgtcgt
     1981 caggtaaggc taatttcatt accagtaaag ggctgttaga agatccctct tcagcgttta
     2041 acagtaagct ggtcatggcg acagtacgca gtcacgatca gtacaacacg acgatttatg
     2101 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     2161 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcactcacgc
     2221 cagacggtaa acgtagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     2281 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaaca cttgataacc
     2341 acgatccatt aagtggcatt cctggctata aaagtattcc ggttgaatta gaaccatcaa
     2401 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat tcctcattga
     2461 ctattatcgc gcaatttgct cacatggatt aaattaaacc acacactata agatataaac
     2521 ttctgcctac agctgtaaga aactccgctc agtactgaag tacccgtcct atttcctcta
     2581 ttctccagcc tgttatatta agc
//
LOCUS       DADSZD010000299         2601 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.301, whole genome shotgun sequence.
ACCESSION   DADSZD010000299 DADSZD010000000
VERSION     DADSZD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.301"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            55..1053
                     /gene="yiaK"
                     /locus_tag="J1Z28_004416"
     CDS             55..1053
                     /gene="yiaK"
                     /locus_tag="J1Z28_004416"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBA7719168.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDNETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            1065..1529
                     /locus_tag="J1Z28_004417"
     CDS             1065..1529
                     /locus_tag="J1Z28_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF386 domain-containing protein"
                     /protein_id="HBA7719169.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            1552..2472
                     /locus_tag="J1Z28_004418"
     CDS             1552..2472
                     /locus_tag="J1Z28_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HBA7719170.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT          711 a          636 c          631 g          623 t
ORIGIN      
        1 cgtatattga aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa
       61 gtgacatttg agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac
      121 aacgaaacgg ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat
      181 tctcacggcg ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt
      241 cctgatgccc aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggacgcccag
      301 cgttcgatcg gtaatctgac agcgaaaaag atgatggatc gcgccattga actggctgcc
      361 gatcacggta ttggtctggt ggcactacgt aatgccaacc actggatgcg cggcggcagc
      421 tacggctggc aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc
      481 gtaatgccac cgtggggcgc aaaagagtgt cgcatcggca ccaacccgct gatcgtcgcc
      541 attccttcca cgccgatcac catggtcgat atgtcgatgt cgatgttctc ttacggcatg
      601 ttagaagtta atcgcctggc aggtcgtcag ctcccggtag atggtggctt tgatgatgag
      661 ggcaatttga ccaaagaacc tggcgttatt gagaaaaatc gccgcatttt gccgatgggc
      721 tactggaaag gttcgggcat gtcgattgtg ctggatatga tcgctaccct cctttctgac
      781 ggcgcatcgg ttgccgaagt caccgaggac aacagcgacg aatacggcat ttcacaaatt
      841 tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc caaactgcaa
      901 cgcattatgg attacgttac cagtgccgag cgtgctgacg aaaaccaggc tattcgttta
      961 cccggccacg aatttactac cctgctggca gaaaaccgcc gtaacggtat taccgttgat
     1021 gacagcgtgt gggcaaaaat ccaggcgtta taaggagata agccatgatt tttggacata
     1081 tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc
     1141 gcaccaccga cttcaacgcc ctggaaccgg gcgttgtcga aatcgacggc aagaatattt
     1201 atgcgcaaat tatcgattta accacacgcg aagcggtaga aaaccgtccg gaagtgcatc
     1261 gtcggtatat cgatattcag tttctggcgt ggggtgaaga gaaaatcggc attgctattg
     1321 atacgggaaa taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc
     1381 acgatagtga acatgaatca tttatagaaa tgattccggg cagctacgct attttcttcc
     1441 cgcaggatgt tcatcgcccg gcatgtaata aaaatgccac tacggcaata cgcaagatcg
     1501 tggttaaagt ggccctcgct gctttataaa aaatgattac tggagcacat aatgaaaacg
     1561 aataatgccg gttatattat cggcgcgtat ccctgtgcac cttcttttca ccaaagaagt
     1621 gaagaggaag agaaggattt ctggcgacaa ttatctgaga ctccagacat tttgggtctg
     1681 gaacaacctt gcctggaaaa ttttcacccg ctgggcgatc aatggttact ccgtcatacg
     1741 ccagagagct ggaaatttgt cgtcaccgca gtgatggaaa ccatgcgtcg ccgcagcgaa
     1801 aacagcgggt ttggtctcgc ctccagtgat gaagaacagc gccaggcctg cgtggcgtac
     1861 tatcgccacc tgttcaataa aatcaactca ttgcaggcta ataaagtgct cgcactggag
     1921 ctccaggctg caccgttagc aacgaatcct aatgttatgc aagcgacgga cgcttttgcc
     1981 cgctcattga aagaaatcgc cagttgggac tggccgtgca agctggtgct ggaacactgc
     2041 gacgccatga catcttcttc cccccgaaaa ggctttttgc ctttagaaaa tgtgctggaa
     2101 gttattaccg attatgacat cagcatctgt atcaactggg cgcgctctgc cattgaaggg
     2161 cgcaatacaa cgctgccgct gacccacacc caaatggcga aacaagccgg aaaactcggc
     2221 gcgttaatgt tctctggtac gacgctaaac ggggcatacg gcgaatggca ggatttacac
     2281 gcaccgtttg caccattctg cgcagaaagc ctgatgacca cagatcatgt tcgcgagtta
     2341 tttaatgtag cagaatcgtc aacattgcat tttgctggga tcaagctact ggaaataaat
     2401 gcgacggctg atgtgcacca tcgcatcgaa atattacgca acggcattca ctctctgaat
     2461 gaatctcgtt aaacagaacc atttatatat cactttgaat atctaattag ccgttttctg
     2521 ataattatta cgaatattaa caacattagc gcataaaaaa tattgcataa ccagaaggac
     2581 tcgttatgaa acggatactc g
//
LOCUS       DADSZD010000300         2576 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.302, whole genome shotgun sequence.
ACCESSION   DADSZD010000300 DADSZD010000000
VERSION     DADSZD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.302"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            194..682
                     /gene="eco"
                     /locus_tag="J1Z28_004419"
     CDS             194..682
                     /gene="eco"
                     /locus_tag="J1Z28_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBA7719171.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(832..2478)
                     /gene="mqo"
                     /locus_tag="J1Z28_004420"
     CDS             complement(832..2478)
                     /gene="mqo"
                     /locus_tag="J1Z28_004420"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBA7719172.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAVDSTPKPQLKPQLVQKEVADIAL"
BASE COUNT          631 a          681 c          627 g          637 t
ORIGIN      
        1 atatagcgat taattcgcca gagatatttc tgctggtttg ttttctcatc agaatttaac
       61 actaaaagag caggtaaaat tatctgaatg ttctttaagt tattcataaa gcaaattaat
      121 aaatctgatg aatatgttaa ccttcagcga catcatcggt gaaaacctat aaatgaagaa
      181 ggaaagcaaa aaaatgaaga ccattctacc tgcagtattg tttgccgctt tcgccaccac
      241 ttccgcctgg gcggcagaaa gcgtccagcc gctggaaaaa atcgcacctt atccacaagc
      301 tgaaaaaggc atgaagcgtc aggtgattca gttaactccg caagaagatg aatctaccct
      361 gaaagtagaa ctgttaatcg gtcagacgct ggaagtcgat tgcaatttgc atcgtctcgg
      421 cgggaagctg gaaagcaaaa cgctggaagg ctggggctat gattattatg tctttgataa
      481 agttagctcg ccggtttcca cgatgatggc ctgcccggat ggcaagaaag agaagaaatt
      541 tgtcaccgcg tatctgggcg atgctggaat gctgcgttac aacagcaagc tgccgatcgt
      601 ggtgtatacg ccagacaatg tggatgtgaa gtaccgcgtc tggaaggcgg aagagaaaat
      661 tgacaacgcg gttgttcgct aaactgctgt gaagtgctgc aacctgtagg ccgaataagg
      721 cggttacgcc gcatccgaca tctaacgccc gagccggttg cctgatgcga cgctgaagcg
      781 tcttatcagc atacgccaca tccggcaaac catgccggat gtggcacatc attacaacgc
      841 aatatccgcc acttcttttt gaacgagttg cggtttcaac tgcggtttcg gcgtactatc
      901 tactgcctgc ggcttgtcgt atttcagccc cagcacttcg ctggtgtact gcaactcgcg
      961 ttctgttgcc gctacatcac cgttcagctt gcgtccataa gacggaacga tcgctttcaa
     1021 cgtagcctgc cattgcgggc tggaaacgcg atcgccaaat actttttcca gcagattcaa
     1081 cataatcggc gcggcggttg acgctcctgg cgatgccccc aggagcgcgg caatggtccc
     1141 ttgctggtca ctgacgactt cagtacccag acgcagtacg ccacctttat cggcatcacg
     1201 cttgataatc tgcacgcgct gccccgcttg ccacaaacgc cagtcctctt ttttcgcctg
     1261 cggatagtac tctttcaacg cttcaaaacg atcctcttca ctcaacatca cctgactcac
     1321 cagatatttc accagatcga aattatccag cccaacgtgc atcatcggca tcacgttaga
     1381 ggtggtggtg gaactcatca gatcccataa tgaaccgttt ttgaggaatt tggttgagaa
     1441 ggtggcaaat ggcccaaaca gcactacgcg tttaccgtca agaacacggg tatcgatatg
     1501 cggaaccgac atcggtggtg cgccaacgga tgctttaccg taaaccttcg ccagatggtg
     1561 attaaccacg tccgggtttt ccgaaacaag gaactgtccg cccaccggga aaccggcgta
     1621 gtctttcgcc tccggaatcc ccgattcctg taacagtttc agcgccgcac cgcccgcgcc
     1681 gataaagaca aatttggcgc ggatgttctg tgcagtgccg tttttcagat cggcaacggt
     1741 gacggtccag ctgttatcgt cattacgctt tagggcgcgg acttcgctgc tgagttgcag
     1801 cgagaagtta gatttcttct gtaaggaggc gatcaactgg cgggtgatct cgccatagtt
     1861 cacatcagtg ccaatttctg tgcgtgttgc cgctactttc tgttgcggat cgcgcccttc
     1921 catcactaac ggtgcccact ctttgatctg cgcgtgatct tcagagtaac gcataccgcg
     1981 aaacagcgag ctttgttgca acgcggcgta acgggcgcgc aggaaattaa cgttatcctc
     2041 gccccagaca aagctcatat gcggaacggt attgataaat gaacgcggag tacgcagcac
     2101 accgcgctcg acctggtgcg cccagaactg gcgggaaatc tgaaatgctt cgttaatggc
     2161 gactgctttt tcaatactga tgctgccgtc ggcattttgc ggggtgtaat tgagttccat
     2221 cagtgcagaa tgcccggttc cggcgttgtt ccagccgttc gaactctcct gcgcgacacc
     2281 ttccaggcgc tccaccatgg tcatcgacca ttcaggctcc agctcgcgta aataggtccc
     2341 caacgtggcg ctcataatgc cgccgccaat caacagtaca tcagtttcct gctcctcgga
     2401 cgctttcgct tttgccgcca tcgaaacggc attaagcccc acggccatcg agaagagcat
     2461 ggcagtcact tttttcatct tgttaatgcc ttacttttag tcgctttatt gcaggtgaga
     2521 tttgcgcggc atcaacggta acacttaagt aacaacattt aaataatgtt tataaa
//
LOCUS       DADSZD010000301         2502 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.303, whole genome shotgun sequence.
ACCESSION   DADSZD010000301 DADSZD010000000
VERSION     DADSZD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.303"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..1240)
                     /locus_tag="J1Z28_004421"
     CDS             complement(<1..1240)
                     /locus_tag="J1Z28_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBA7719173.1"
                     /translation="MALTDIKVRAAKPADKQYKLTDGGGIHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARKLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWATELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSCRGS
                     KMRTMHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYA"
     gene            1433..1885
                     /locus_tag="J1Z28_004422"
                     /pseudo
     CDS             1433..1885
                     /locus_tag="J1Z28_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
BASE COUNT          673 a          602 c          551 g          676 t
ORIGIN      
        1 ttgcatattc aaatggactg ataaaccttt ctctgtttac atccagaaaa tcggcccacc
       61 actgtagcat caatcgccgt tcttccagat gctctgcttt atggatatac gcggccctca
      121 ctgaatttcg ctccatgtgg ctcatctgac gttcaacagc atcacgagac cacaaacctg
      181 attcgaccaa tgaactacag gccattgttc gaaagccatg accacaaacc tctacttttg
      241 tatcataccc catgacccgt aacgcactat ttaccgtatt ctcactcatg ggtttgtgcg
      301 aatcgtgatc accaataaat atcaagtcat gggccccata aaactgtttt atctgcttta
      361 aaattgcaag agcttgcgtt gaaagaggca ctagatgcat tgtacgcatt tttgagcctc
      421 tacaggaatg tttcactcca ggaataggct cccgctccgg tgggatagtc catatagacg
      481 cttcgaaatc gatctctgac caacgagcaa aacgcagctc actggaccga ataaagatca
      541 gcaaagtgag ttctgtcgcc catcgggtta gcggcctacc agtatagcta tctatttttg
      601 taagcaactc agggatgcgc tttaattcaa gcgcgggacg atgttgtcga ttacaggaag
      661 caaccgcccc agccatctct tgtgccgggt tataatcaat taacccactt tgcactgcat
      721 agcgcatgat ggctgtagtg cgctgctgaa gacgagcggc cacttcaaga cgtccagaca
      781 tttctacggc cttaataggt gctaataaat ctcgagtttt taactcagcg atattacgtt
      841 caccaagcgc tgcaaaaaga ttatcttcaa gactttttag cacacgatgg gcgtgatctt
      901 cagaccactt tttattggtg ccatgccact caatcgcgac ttctttaaag gttcgtgctt
      961 tactctgttc aaccttatca tttttctttt tgtctcccgg atcgacgcca ttcgcaagca
     1021 gcttacgcgc ctcgtcacga cgtactctgg catccgctag tgtgatttca ggataaaccc
     1081 caagtgccag cattttttgc tttccctcat aacggtactg caaacgccag tacttagaac
     1141 catttggatg gacaagcaga tgaataccgc caccatcagt cagcttatat tgcttatccg
     1201 ctggcttggc tgctctgact ttgatatctg ttaatgccat gcgtattccc tccctgttgg
     1261 tacaagcatt atcgaaccgg aaataccatc atctgtacca acatatgttg gtagatgtac
     1321 gttgatatcg gttgaccttg agagagttaa tatagcggga aagatagata aacactggat
     1381 ttcaggcata aaaaaagacc tcagttgagg tctatttaca tacttttggt gcgaaggcag
     1441 aaacgtcagt cgcctggacg aattacgcaa tattatcgaa gtcagaaaat ggcaggtgaa
     1501 tcaggccgcc ggtcgttata ttcgttcgca tgaagccgtt cagcacatca gcatccgcga
     1561 ccggctgaat gattttatgc agcagcacgg cacagcactg gcggccgcac tggcaccgga
     1621 gctgataggc tacagtgagc tgacggccat tgcccgaaac tgtgccatac agcgtgccac
     1681 agatgccctg cgtgaagccc ttctgtcctg gcttgcgaag ggggaaaaaa ttaattattc
     1741 cgcacaggat agcgacattt taacgaccat cggattcagg cctgacgcgg cttcggtgga
     1801 tgacagccgt gaaaaattca ctcctgcgca gaacatgatt ttttcgcgta aaagtgcgca
     1861 actggcatca cgtcagtctg tgtaaaattc cccgaaaatc cgcctgtttt tactgaaaaa
     1921 agccatgcat cgataaggtg catggctttg catgcgtttt cctgcctcat tttctgtata
     1981 ccgcgccatt cccggcacgg cctgagcgtg tcagtgcaac tgcattaaaa ccgccccgca
     2041 aagcgggcgg gcgaggcggg gaaagcaccg cgcgcaaacc gacaagttag ttaattattt
     2101 gtgtagtcaa agtgccttca gtacatacct cgttaataca ttggagcata atgaagaaaa
     2161 tctatggcct atggtccaaa actgtctttt ttgatggcac tatcctgaaa aatatgcaaa
     2221 aaatagattg atgtaaggtg gttcttgtca gtgtcgcaag atccttaaga attcgtggca
     2281 tgagagagtt aaaggatgct gaatcatgta tatggattaa ttggcgttgt tgggaccata
     2341 atcactattg ctactagctt catccgcaac caagatattt cacaatggtt gctcgtttgt
     2401 tctggatggt tagctgcttt acttattgga tggtttacac acagaacgat taaagcaatt
     2461 agcaacaacc acacgaatgt tataaaaagt aatatggagg ta
//
LOCUS       DADSZD010000302         2488 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.304, whole genome shotgun sequence.
ACCESSION   DADSZD010000302 DADSZD010000000
VERSION     DADSZD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.304"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1851
                     /locus_tag="J1Z28_004423"
     CDS             <1..1851
                     /locus_tag="J1Z28_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBA7719174.1"
                     /translation="KYTSVVDLARARANQQYTENSTGKISPVIAAIHREYKQTWKTLD
                     DELAYALWPGDVDAGNIDGSIHRWAKKEVIDNDREDWKRISASMRKQPDALRYDRQTI
                     FGLVRERPIDIHKDPVALNKYICEYLTTKGVFENEETDLGTVDVLQSSETQTDAVETE
                     VSDIPKNETAPEAEPSVEREGPFYFLFADKDGEKYGRANKLSGLDKALAAGATEITKE
                     EYFARKNGTYTGLPQNVDTAEDSEQPEPIKVTADEVNKIMQTANISQPDADKLLAASR
                     GEFVEGISDPNDPKWVKGIQTRDSVNQNQHESERNYQKAEQNSPNALQNEPETKQPEP
                     VAQQEVEKACTACGQTGGGNCPDCGAVMGDATYQETFDEEYQVEVQEDDPEEMEGAEH
                     PHKENTGGNQHHNSDNETGETADHPIKVNGHHEITSTSRTCDHLMIDLETMGKNPDAP
                     IISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREAQSAIMTDEIP
                     LDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIPCPWRYYNDRDVRT
                     IVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADS"
     gene            1939..2175
                     /locus_tag="J1Z28_004424"
     CDS             1939..2175
                     /locus_tag="J1Z28_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBA7719175.1"
                     /translation="MIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREYKHVAH
                     DGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            2195..>2488
                     /locus_tag="J1Z28_004425"
     CDS             2195..>2488
                     /locus_tag="J1Z28_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBA7719176.1"
                     /translation="MREVEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     VAGELRSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKE"
BASE COUNT          787 a          562 c          618 g          521 t
ORIGIN      
        1 aaatacacgt ctgttgttga tctcgcccgc gccagagcca atcaacagta cactgaaaat
       61 tcaacaggaa aaatcagccc ggtcattgct gccattcatc gcgaatacaa gcagacatgg
      121 aaaacactgg atgacgaact ggcctacgct ctctggcctg gtgatgtgga tgccggaaac
      181 attgacggca gcatccatcg ctgggcaaaa aaagaagtta tcgacaacga ccgcgaagac
      241 tggaagcgta tctcggcatc aatgcgcaaa cagcctgatg cccttcgcta cgaccgccaa
      301 actatttttg gccttgtccg tgagcgtccg atcgacattc acaaagatcc cgtagcactg
      361 aacaaatata tctgcgaata cctgacgaca aagggcgtgt ttgagaatga agaaacagac
      421 ctgggcactg ttgatgttct tcagtcatca gaaacacaaa ctgatgcagt ggaaactgag
      481 gtatctgata tcccaaaaaa tgaaaccgcg ccggaagctg aaccatctgt agagcgtgag
      541 gggccgttct atttcctctt cgcagataag gacggagaaa aatacggtcg cgcaaacaaa
      601 ctttctggtc tggataaggc actggctgct ggcgccactg aaatcacaaa agaagaatat
      661 tttgcccgaa aaaatggcac atacacgggc ttaccgcaaa atgtagatac cgctgaagat
      721 tcagaacaac cagagccgat aaaagttacc gctgacgaag taaacaaaat tatgcagaca
      781 gccaatatca gccagcctga cgccgataag ttgcttgctg catcacgtgg tgaatttgtt
      841 gaagggatta gtgacccgaa tgatccgaaa tgggttaagg ggatccagac ccgcgattct
      901 gtgaaccaga accagcatga atcggaacgg aactaccaaa aagcggaaca aaacagtcca
      961 aatgcgttac aaaacgagcc agaaacgaaa cagcctgaac cagtggcgca acaggaagtg
     1021 gaaaaagcct gcaccgcctg cggtcagacc ggcggcggca actgccctga ttgtggcgcg
     1081 gtgatgggcg acgcaacata ccaggaaaca ttcgatgaag agtatcaggt tgaagttcag
     1141 gaagatgatc cggaggaaat ggaaggcgct gaacatccac acaaagagaa cactggcggc
     1201 aatcagcatc acaatagcga taatgaaact ggcgagacgg cagatcaccc aattaaggtg
     1261 aacggtcatc acgaaatcac atccaccagc aggacgtgtg accatctaat gatcgacctt
     1321 gaaaccatgg gaaaaaatcc tgatgccccg atcatctcaa taggtgcaat atttttcgat
     1381 ccgcaaaccg gagatatggg accggaattt agtaagacta tcgatctgga aactgctggc
     1441 ggagtcattg atcgggacac cattaaatgg tggcttaagc aatcacgcga agcgcaatct
     1501 gccattatga ccgatgaaat cccgttagat gatgcactgt tacaattgcg ggaatttatc
     1561 gacgaaaact ccggtgaatt ttttgttcag gtttggggaa atggagccaa cttcgacaac
     1621 acgattttgc gccgttcata cgaacggcag gggatcccct gcccgtggcg ttactacaac
     1681 gatcgcgatg tacgcacaat cgttgagctg gggaaagcca tagacttcga tgccagaacg
     1741 gctattccat tcgaaggtga gcgccataat gcacttgatg acgcccgtta ccaggcaaaa
     1801 tacgtttcag ttatctggca aaaactgatc ccgagtcagg ctgattctta atgttcaact
     1861 gtcgccggtt gtgactggta ttctgcaacc ggcgctcgtc tgatgtaaga gataaagaaa
     1921 tcgatgagcg aagtaatcat gattgtctct cccggcaaat gggtatccga agagcagtta
     1981 attgcgctga aaggaataaa aaaaggtacg ttaaaaaagg cccgggaaaa atcgtttatg
     2041 gaaggaaggg aatataagca tgtcgctcat gacggtatgc catgggataa cagtccatgc
     2101 ttttacaacc tggaagaaat tgatcgctgg attgagcgcc aggcatcagc gagaccaaga
     2161 cgtcatctta cttgactaaa agccacacta actaatgaga gaagttgaaa tgaaatatcc
     2221 gacaggcgtg gaaaaccatg gagggaaatt acgtatctgg tttgtttata aaggcgtaag
     2281 agtcagggaa aatctggggg ttcctgacac agcaaaaaac aggcgcgttg caggtgaact
     2341 acgctcctct gtttgttacg caataaaaac tggtgttttc gactatgcaa aacagtttcc
     2401 ctcctcacgc aatctggaaa aatttggtga ggcccgacaa gatttaacca taaaagaact
     2461 ggctgaaaaa tttctggcac tgaaagaa
//
LOCUS       DADSZD010000303         2474 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.305, whole genome shotgun sequence.
ACCESSION   DADSZD010000303 DADSZD010000000
VERSION     DADSZD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.305"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..69
                     /locus_tag="J1Z28_004426"
     CDS             <1..69
                     /locus_tag="J1Z28_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBA7719177.1"
                     /translation="LAELTMNKLYDRVPASVWKYVT"
     gene            301..900
                     /locus_tag="J1Z28_004427"
     CDS             301..900
                     /locus_tag="J1Z28_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA7719178.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTGGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYALSYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGSPLPETYSLV"
     gene            1271..1654
                     /locus_tag="J1Z28_004428"
     CDS             1271..1654
                     /locus_tag="J1Z28_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719179.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQAAPYARYTCHLCGSALRYHPQY
                     DTECPGFEHADDGLTEYGHECPYVRPERREIQLIKCLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
     gene            1651..2076
                     /locus_tag="J1Z28_004429"
     CDS             1651..2076
                     /locus_tag="J1Z28_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719180.1"
                     /translation="MNGIKTGLGMTPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFQDCGGAST"
     gene            2278..>2474
                     /locus_tag="J1Z28_004430"
     CDS             2278..>2474
                     /locus_tag="J1Z28_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBA7719181.1"
                     /translation="MVSTNHTRESSPYLSVPQDDFCIVNPGGCIIHFPANGRYILDIH
                     DPDFPEQYQRLLERLDYLTKPG"
BASE COUNT          585 a          613 c          641 g          635 t
ORIGIN      
        1 ctggcagaac tgaccatgaa caaactgtac gatcgtgttc ccgcttcagt ctggaaatat
       61 gttacctgat aaatgtttct gatgaagtac agtcgtcaca atgacatcac ttcagatatt
      121 atctgcgctg tgactgactg tcacgtatgc cgttctctgt atggttctga tacccgggtg
      181 tggtctcact tcctctgtgg cgcccgggaa gttgtgaact tgcacgtctt cgtgtgataa
      241 cgttttcttc aaaaattacg ccagcgcttc ttcctgtgtt acagaacacg aggcagaaac
      301 atgaagactt acaacaccaa tcccggttac gaaatggatc cccagcttct gacacacttt
      361 aaccagcatc tggattcatt attcggggta tattcgaaac tgttaccgtt cagaatggat
      421 tttgcttacc gaaaaaacac cctcagctac aggtgtgcct gcaggtacgc catgtgcgcg
      481 gagatattgc gactgattaa cgaagtggga gagaaactgg tcgggtatgc ctgggtaatg
      541 gaatatacgg aacgtaaagg gcttcacatt cattttgtgg gctatctgaa cggtcagagc
      601 cacaggagtt cgtatctggt atccagattg atgggagata tctggaggcg ggttaccggg
      661 ggggatggtt actatcactg gtgccggttt aataaaaatt atccggtaaa cattaaccac
      721 gtcattcact attcagatca caaagcggtg aatgccttac gctatgccct cagctacctg
      781 gccaaacgag aacagaaaga atgtggcatc atcctgaaat gcagcgggct tcctgaaaaa
      841 agtaatcggg gtcgaccgcg actgggcagc cccctgccgg agacttattc tctggtgtaa
      901 gaccctgaac gacaactacg ggctggcgct aattgttaca gtcggttccg gaggccactt
      961 cagggactct gcgcattttt tacgacatgg ctattccgta tgaaccatac ggagatttaa
     1021 ccatggcaca ttctttacca ttccttccgg caaccgatac agcacctcct ccgatgtcca
     1081 tctctgcttg ctgactgttt tttacgtcag tgggccattg tgtgacaacc acagtgctgt
     1141 aaaagcaacc agcctgcttt acccaaaccc cagtccagat tttaactgag ccgccattca
     1201 cacctgagca cgctgcccgt accgggacgg ctgttgcctg cgtgtcgtct ggcggctgag
     1261 gaatatgacg atgtacgcaa aatcctttat tgcatttgat ggcaacggac gtctgaccgg
     1321 cgcacgtact gcacaggccg caccttatgc ccgctacacc tgccaccttt gcggcagtgc
     1381 actcagatac cacccgcaat acgacactga atgtcccggg tttgaacacg ctgacgacgg
     1441 gctgactgag tatggtcatg agtgccctta tgtcaggccg gagcgcagag aaatacagtt
     1501 gattaaatgt ctgcagcaat tcgtaccgga tgccttaccc gtggtgcgta aagccagctg
     1561 gcactgcaga caatgtcatc acgattatta tggggagcgg tactgcactc actgccatac
     1621 agggcacttc agtgaggagg gagtggctga atgaacggta taaaaacggg gctggggatg
     1681 acgccgggtg aacacattat cagtgccgac tctgcgctca gcagaaatat ccggcagtgt
     1741 ttctgccttt cctgtcgtgg ccggttgatt ttgcagacag atgctcaggg ggcgtggttt
     1801 gagcatgatt tacatgccct gagcgcgcaa cagaaggcgg cctgcgtggt gctgaatccg
     1861 gaaaagtccc acccttacat tgaggatatg gcgatgtttt tatcgccatt accggtcgtg
     1921 ctggagtggc attgtgtgat gtgtgagcag ttttttcatg ggaagaaatt ctgtgaggcc
     1981 tgtggtacgg ggatttattg cagagcggta tgtacgaagt ctgtttacag ctaccagcca
     2041 gacttgtttc aggactgcgg aggtgcgagt acttaatccg aaatgcccga cctgctcagg
     2101 gcgttcagat gtatcggaac ctgcatttta tctggtgttt cgcacccacc ccgttcgtga
     2161 cacaaaggcg cgacaccgga tatccgcagt cctgcttacc tgaataaaac ccggggtatt
     2221 gattgtgtac tgtggtgcga aatcgcaccg tgatcgcgga ttcgccaaat ccgcactgtg
     2281 gtgtcaacca accatacgcg agagtcctct ccgtatttat cagtgcctca ggacgatttc
     2341 tgcattgtta atccaggggg ctgcattatt catttccctg ctaatggacg ttacatcctg
     2401 gatattcatg atcctgattt ccctgagcag tatcagcgct tattggagcg tctggattat
     2461 ctgacgaagc ccgg
//
LOCUS       DADSZD010000304         2464 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.306, whole genome shotgun sequence.
ACCESSION   DADSZD010000304 DADSZD010000000
VERSION     DADSZD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.306"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            332..475
                     /locus_tag="J1Z28_004431"
     CDS             332..475
                     /locus_tag="J1Z28_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBA7719182.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            488..697
                     /gene="cspE"
                     /locus_tag="J1Z28_004432"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             488..697
                     /gene="cspE"
                     /locus_tag="J1Z28_004432"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBA7719183.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            863..1672
                     /gene="rlmA"
                     /locus_tag="J1Z28_004433"
     CDS             863..1672
                     /gene="rlmA"
                     /locus_tag="J1Z28_004433"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBA7719184.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDAKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1669..2235)
                     /gene="mntP"
                     /locus_tag="J1Z28_004434"
     CDS             complement(1669..2235)
                     /gene="mntP"
                     /locus_tag="J1Z28_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBA7719185.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          653 a          579 c          601 g          631 t
ORIGIN      
        1 tgttttatga gaaaaagtga tataactttt tattcattgc atagtagaaa atgtgatatt
       61 gcacgcacta tgtaataact tctcccactg gcctggaaca actgaactta ttgaactatg
      121 ttagaaaata cgtcagttta agtatctgcc tgaactggca aggttaagca caatgatata
      181 tcggcgcgta ttccgttgca taagtgtgca aaaaaagtgg aagacgtatc gagatttgtg
      241 cgtctgatcg agacatgttt aaaaatggct tgccataatt aacgttgtat gtgataacag
      301 atttcgggtt aaacgaggta cagttctgtt tatgtgtggc attttcagta aagaagtcct
      361 gagtaaacac gttgacgttg aataccgctt ctctgccgag ccttatattg gtgcctcatg
      421 cagtaatgtg tcagttttat ctatgttatg cctgcgggcg aagaaaacaa tctaaggaat
      481 ttttcaaatg gcaaagatta aaggtcaggt taagtggttc aacgagtcta aaggttttgg
      541 cttcattact ccggctgatg gcagcaaaga tgtgttcgta cacttctccg ctatccaggg
      601 taatggcttc aaaactctgg ctgaaggtca gaacgttgag ttcgaaattc aggacggtca
      661 gaaaggtccg gcagctgtta acgtaacagc tatctgatcg aatccactga tctgaagtgt
      721 gaatacgctt caatctcgct ataaagcctc gtcgaatgcg aggcttttta ctttgtttta
      781 tcttcgctcc agtcgttcgg atatttgccc gccgcatgat tcgcgttaca cttgcggcct
      841 ttagtatcct gccggagttg tcatgtcgtt ttcctgtcca ctttgccatc agcctctttc
      901 gcgtgaaaaa aacagctata tctgtcccca gcgacatcag tttgatatgg cgaaagaagg
      961 gtatgtcaat ctgctgcccg ttcagcataa acggtctcgt gatccgggcg acagcgcgga
     1021 aatgatgcaa gcacgccgcg cattcttaga tgccggacat tatcagccgc tgcgtgatgc
     1081 aattgtcggc caactgcggg aacggcttga tgcaaaggcc gcggcggtgc tggatattgg
     1141 ctgtggtgaa gggtattaca cacacgcatt tgctgatgcg ttgcccgaaa tcaccacgtt
     1201 tggtctggat gtctcgaagg tagcgataaa agcggcggcg aaacgctatc cgcaggtcac
     1261 tttttgtgtt gcttccagcc accgtttgcc gttttccgat accagtatgg acgccataat
     1321 acgtatttac gcgccgtgta aagcagaaga actagcgcga gtagtgaagc ccggcggttg
     1381 ggtcattact gccacgccgg gaccgagaca cttaatggag ctgaaggggc tgatttacaa
     1441 tgaagtacat cttcatgcac ctcatgcaga acaactgaaa ggttttacat tacagcagag
     1501 tgatgagttg tgttatctga tgcgtcttcg cggtgatgaa gccgtcgcat tattgcagat
     1561 gacgccgttt gcctggcgtg cgaagcctga agtctggcaa gcactggcag caaaagaagt
     1621 gttcgactgc cagacggact ttaatattca cctctggcag cgttcttatt aaccgtggaa
     1681 gtgcgtccag aggatctgga cgccgatgcc gatcagtacc agcccgccga gaatttccgc
     1741 ttttttccca ataattgagc cgataaagcg accaaccatc atccctaatg tcgacataat
     1801 caaggttgca caaccaatgg ccaatgcggt cgcgataatg ttaacctgca ggaaagcaag
     1861 accaacaccc acagccatgg catccaggct ggtggcaatc gcggtggtta ccagtagcca
     1921 gaaaccgtgt cgacggcgcg gttcttcatc ttcatcatct gcgccacgaa aaccctcaat
     1981 aatcattcgc ccgccgagaa atatcagcag cacaaacgca atccagtggt tccattcaag
     2041 gacaaaccga ctggctaaca tgcccattcc ccagccgatc agcggcgtca gggtttcgac
     2101 ggcaccaaaa ataaggccgg ttcgcaatgc ttcagaaaat ttcggtttat ggagggtggc
     2161 acctttaccg attgatgcag caaaggcatc catcgacata ccaaacgcaa gaagaacagt
     2221 agcagtgata ttcatgacaa cgtcctgacc ggggtatcca taaaacacat catcgccccc
     2281 agcaaacatg catacatgaa caacaagccg cgatggtttt cgcgttcata caggcatagc
     2341 gattgatgtg catatggtct cgcctgactg aaagtgttca gtccgtacgt gccacgtttt
     2401 gcaacgagta tgttgacacg tacatttccg gaatctggaa atcagctact ccccaaatga
     2461 gggc
//
LOCUS       DADSZD010000305         2462 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.307, whole genome shotgun sequence.
ACCESSION   DADSZD010000305 DADSZD010000000
VERSION     DADSZD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.307"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            102..785
                     /locus_tag="J1Z28_004435"
     CDS             102..785
                     /locus_tag="J1Z28_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBA7719186.1"
                     /translation="MNLKKTLLSVLMILQLCLLVGCDYIEKASKVDDLVTQQELQKSK
                     IEALEKQQELDKRKIEHFEKQQTTIINSTKTLAGVVKAVKDKQDEFVFTEFNPAQTQY
                     FILNNGSVGLAGKILSIDAVENGSVIRISLVNLLSVPVSNMGFYATWGGEKPTDINAL
                     AKWQQLLFSTSMNSSLKLLPGQWQDINLTLKGVSPNNLKYLKLAINMANIQFDRLQPA
                     ESPQRKNKK"
     gene            829..>2462
                     /locus_tag="J1Z28_004436"
     CDS             829..>2462
                     /locus_tag="J1Z28_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBA7719187.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVAGG
                     LLSSFGAYAEVSLDGGTSAETPALDNVWIAIGKGSKASSVSAGGTASVAIGVQAEANV
                     GSTALGFKSLATGEKSVSIGQLAIASGSRGVAIGSAANASASNTIAMGLLAKAGGDGS
                     KQGAMAMGAYSEANGSYSIAFGDHAKSQGDYGISLGETAGANGLYSLALGKESNAIGD
                     DSLALGRLSLASAANAIAMGADSRSSNDNAIAIGNKTQALGVNSMALGNASQASGESS
                     IALGSTSEASEQNAIALGQGSIASKVNSIALGSNSLSSGENAIALGEGSAAGGSNSLA
                     FGSQSRANGNDSVALGVGAAAATDNSVAIGAGSTTDASNTVSVGNSATKRKIVNMAAG
                     AISNTSTDAINGSQLYTISDSVAKRLGGGATVGSDGTVTAVSYALRSGTYNNVGDALS
                     GIDNNTLQWNKTAGAFSANHGANATNKITNVAKGTVSATSTDVVNGSQLYDLQQDALL
                     WNGTAFSAAHGTEATSKITNVTAGNLTAGSTDAVNGS"
BASE COUNT          749 a          539 c          619 g          555 t
ORIGIN      
        1 caaatttatt ttgcggatat gccccactcc aatcattcat aaaaaaaatg attgtgccgt
       61 aattatcaac tgtatcggga ttatgaaaag gaattcatcc tatgaaccta aagaaaacac
      121 tgttaagcgt gttaatgata ttgcaacttt gcttattggt agggtgtgac tatattgaaa
      181 aagcgagtaa ggtcgacgat ctcgttacac agcaagagtt acaaaaaagc aaaattgagg
      241 cgcttgaaaa acaacaagaa ctcgacaagc gcaagataga acactttgaa aaacaacaaa
      301 ctaccatcat aaacagtacc aaaacgctcg ctggcgtggt gaaggcagtt aaagacaaac
      361 aggacgaatt tgtctttaca gaatttaacc cggcacaaac ccaatacttc attttaaata
      421 acggttctgt tggtttggca gggaaaatac tgtctattga cgcagtagaa aacggcagcg
      481 ttattcgtat ttcactggtt aacttattaa gtgttcctgt atcaaatatg ggtttctacg
      541 cgacatgggg gggagaaaaa cccaccgaca tcaacgcatt agcaaaatgg cagcaattgc
      601 tatttagtac ctcaatgaac tcctccctga aattattacc aggtcaatgg caagacatta
      661 atttgacgct aaaaggtgtc tcgcccaaca acctcaaata tctgaaatta gccatcaaca
      721 tggcaaatat tcagttcgac cgtcttcaac ctgctgaatc tccacagcgg aaaaacaaaa
      781 aataaatgca gcttctgcgc ttatatcaag gaatagagag catcaataat gaacaaaata
      841 tttaaagtta tctggaatcc ggcgacaggc agttacaccg ttgccagtga aacagcaaaa
      901 agtcgtggta aaaaaagcgg gcgcagtaag ctgttaattt ctgcactggt tgcgggtggg
      961 ttgttgtcgt catttggagc atatgccgaa gtatcacttg atggtggtac aagtgctgaa
     1021 acgcccgcgc ttgataatgt ctggattgct ataggtaaag gttcgaaggc ctcatcagtg
     1081 tcagcaggag gaactgcgag tgttgccatt ggggtacaag ctgaagcaaa tgtagggagt
     1141 acagcattag gttttaaaag cttagctacg ggtgaaaaat cagtatctat aggacaattg
     1201 gcgatcgctt caggaagtcg aggtgtcgca ataggaagtg cggcgaatgc ttccgcatca
     1261 aatactattg ctatgggtct attggctaaa gctggcggtg acggctccaa gcaaggtgct
     1321 atggcaatgg gcgcatactc agaagcaaat ggcagttact ctatcgcttt tggcgatcat
     1381 gcgaagagtc agggcgacta cggaatatct cttggagaaa ctgccggggc caatggccta
     1441 tatagcctgg cactaggtaa agaaagtaat gcaatcggtg atgacagcct ggcattaggc
     1501 agattatcac ttgccagtgc agcgaacgct attgcgatgg gggcagacag tcgcagttcg
     1561 aacgataacg ccatcgcgat tgggaacaaa acgcaagccc tgggagtgaa ttcgatggcc
     1621 ctgggtaatg caagtcaggc atctggcgaa tccagtattg cattaggtag caccagtgag
     1681 gccagcgaac aaaatgcgat tgcgctgggg caaggtagca ttgcaagcaa agtgaactca
     1741 atcgcgttgg gaagtaacag tttgtcctcg ggagagaatg ccatcgcatt gggagagggt
     1801 agtgccgctg gtggcagcaa cagccttgct ttcggtagcc agtccagggc aaacggcaat
     1861 gattctgtcg ccctcggtgt aggggctgca gcagcgaccg acaattctgt cgctatcggc
     1921 gcaggatcga ccacagatgc aagcaatacg gtttcagttg gcaacagcgc aacaaaacgc
     1981 aaaattgtta atatggcagc tggtgccata agcaacacca gtaccgatgc catcaacggc
     2041 tcacagcttt atacgatcag tgattcagtc gccaagcgac tcggaggagg cgctactgta
     2101 ggcagcgatg gcaccgtaac cgcagtaagc tacgcgttga gaagcggaac ctataataac
     2161 gtgggtgatg ctctgtcagg aatcgacaat aataccctac aatggaataa aaccgcgggg
     2221 gcgttcagcg ccaatcacgg tgcaaatgcc accaacaaaa tcactaatgt tgctaaaggt
     2281 acggtttctg caaccagcac cgatgtagta aacggctctc aattgtacga cctgcagcag
     2341 gatgctctgt tgtggaacgg cacagcattc agtgccgcac acggcaccga agccaccagc
     2401 aaaatcacta atgtcaccgc tggcaacctg actgccggca gcactgacgc cgttaacggc
     2461 tc
//
LOCUS       DADSZD010000306         2452 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.308, whole genome shotgun sequence.
ACCESSION   DADSZD010000306 DADSZD010000000
VERSION     DADSZD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.308"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(68..241)
                     /locus_tag="J1Z28_004437"
     CDS             complement(68..241)
                     /locus_tag="J1Z28_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719188.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLCQVA
                     ESTPDIPKAPWEI"
     gene            complement(389..2374)
                     /gene="pdeR"
                     /locus_tag="J1Z28_004438"
     CDS             complement(389..2374)
                     /gene="pdeR"
                     /locus_tag="J1Z28_004438"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBA7719189.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSMFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT          679 a          593 c          561 g          619 t
ORIGIN      
        1 taatactaaa taaaaaaata ctgacgcgcc gggcatcctc aaataggaaa acctgaaggc
       61 acgattgtta gatctcccac ggtgccttcg ggatgtctgg cgtagattca gcaacctggc
      121 acaagagttc tgctttaagt atctcgagat tatggatcgc agagtggtaa tcgcctgcca
      181 caaggatatc taacacaata tcaatacgtt cggcaacgcg ctgggcatca aattcagaca
      241 tcaaggacat ccttatagca aaagggggaa aatgaataat gcaaaaattg ccgctaaaag
      301 agaagtgttt aacagcaacg gctaattatc atccgggaat acgatatata atgacggaat
      361 atagtgctaa gtatatatat tcatctactt atgcgcgctt cagatagcgt ttataccagc
      421 gttcgaaggc aacggcgggc atcggtttgg caaacaaaaa tccttgtcgc tcattgatcc
      481 cgtttttggt taaaaaagca tcttcctttg cactttctac accttcggcg atcacctgaa
      541 gattcaatgc ctgggccaca gcgacgatcg cccggaccag tgattgcgag acaggctgtt
      601 tgtgaatatc ccgaacaaaa acctggtcaa gtttgatggc atcgatcgga aagcgcgcca
      661 gttgcgaaag tgaagagtag ccggtaccaa aatcgtccag atgaacttgc gcacctaatc
      721 ggctaaattg ttgaataaca gacagtgcca gttcatcatt ctcaatcagg cagctctccg
      781 tcagttcaac atctataggg cagtattcaa aattgagttc ctggagaacc tgtttcaggg
      841 cggtgaaaat ggtttgatcg gcgagctgac gtgcagaaat atttaccgcc actcgcaggt
      901 taatgccttt atcccgccac ttcgctacct ggcgtacgac atcgagaatc acccaacggc
      961 ctaaaggcac aattagccct gattcttcgg cgtaggaaat gaagtccaac ggtggaatca
     1021 acccacgttc aggtgactgc caacgtacta gtgcttccag actgcgcact tcgccacgcc
     1081 aggtgatttt cggttgatag tgaataacca actgatcgtt ttccagtgct ttacgcaagt
     1141 tggtatccag ccagagatat tcaaataccc gctgattcat ttctggggta aaaacgcaaa
     1201 attgtcctcg tccgccttcc ttcgctgtgt acattgctgt gtcggcgtga cgaataatag
     1261 ccgcgctgtc cgaaccatgt tcgggagaga gtgaaatacc tactgaacag ctggtataaa
     1321 cttcaattaa accaatgcga aaggggagtc gtaagcgggt caaaattcgt gatgccaatg
     1381 cttccagcgc gctttgtgag gtattggatg ccagtaccag aaactcatcg ccacctggac
     1441 gcgccaatac ctggtcatgt tcgagacagc ttaaaatagc caatgacacg tcgcgtaata
     1501 actggtcacc aaacaaatgc ccataggcgt cgttgacctt tttaaaatta tccaaatcaa
     1561 gatacacaac cccaacttta ttgttatctg cctgattaat agcgtgatcg attaactcct
     1621 gcatcgcgtt acgattcggc agtccggtga tactgtcggt atttgccaga atacgcagtc
     1681 gctcctgagc gcggcgctct tcggtaatgt cggtgccgga acagattaaa aaaatctcgt
     1741 ttttgccact gccgctgtgg acaaatttat tgcgaaacag aaacagccgc tggcctttac
     1801 gtgttggtat ccatagttcg acttcatatg cattgccgct tcgaaaaaat acacggttat
     1861 tgcgcctgga tgccgcagct tcacgacgac tcataaacag tttaaacatg ctttgcccaa
     1921 tgacgtcgtg ttctttcaag cctgtgtaat cttcacataa ccgattaaag cgctggatat
     1981 tcccccggct gtcgagaatc actattgcgg aatgtgcttc agaaactacc tgctcagcaa
     2041 aggaaagccc atgtgataaa tcacgagcaa ccgccggtgt gtcatgccat gcagacgcgt
     2101 tgcctcccca ttctcgttta ttgatttttc gtcctaccag atgcacagaa agatcgcttt
     2161 catcgacggt cagactcatc atcaggctgg aggtgatgac cgtcatttcc ctgatgcgag
     2221 cagcctgttc ggcagatagt tgcaaaatac gatcgggttc tgtgatttcg gtggtagaaa
     2281 agcgcaaaac atcgctgctt tccgtcaacc gccagtatgg attgtgcgac ccgagaaagt
     2341 tgtacaacgt tgtggactcc ctaacggttt tcatgatgca tatcctccgg ttaacagcgg
     2401 agtccgcagt taaataaaag gaacaacatg acttacatga aattaacggc gg
//
LOCUS       DADSZD010000307         2381 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.309, whole genome shotgun sequence.
ACCESSION   DADSZD010000307 DADSZD010000000
VERSION     DADSZD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.309"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            44..739
                     /locus_tag="J1Z28_004439"
     CDS             44..739
                     /locus_tag="J1Z28_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBA7719190.1"
                     /translation="MLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVHKPAPSMPVIQD
                     VPRNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFTGICYSHRVFVA
                     LNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGVHDHFGVSWFNP
                     EDLATKRKAMARGLRKILLARHALFDEVASASLAPLSAFDVLISDRPLPADYVTHCRN
                     ASVKIITPDSEDE"
     gene            complement(741..839)
                     /locus_tag="J1Z28_004440"
     CDS             complement(741..839)
                     /locus_tag="J1Z28_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HBA7719191.1"
                     /translation="MISQIDKLEYVMKVQRNQSNPTMFNKSTVFFQ"
     gene            complement(2134..>2381)
                     /locus_tag="J1Z28_004441"
     CDS             complement(2134..>2381)
                     /locus_tag="J1Z28_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3296 domain-containing protein"
                     /protein_id="HBA7719192.1"
                     /translation="NDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLV
                     FDAANTYNAEPGVLEQTARAYIESHGAVERHLGCSDF"
BASE COUNT          643 a          593 c          528 g          617 t
ORIGIN      
        1 acttaagaaa accgacagaa tccatttgaa agacgcggca cgaatgctgg aagtttctgt
       61 aatgactatt cgtcgtgatc tccatcagga agatgaacct ctgccactga ccctactggg
      121 tggctatatt gtaatggtgc ataaacccgc accatccatg ccagtaatcc aggacgttcc
      181 gagaaatcat cgtgatgact tacctattgc aattctggcc gccggaatgg ttaatgaaaa
      241 tgatctgatc ttctttgata atggccagga gataccgctc gttataagca tgatcccgga
      301 tgcaatcacc ttcactggca tctgttactc acatcgtgtc tttgttgcgt tgaatgaaaa
      361 acctaatgtg acagcaatac tttgtggtgg tacgtatcgt gccagaagtg atgcttttta
      421 cgatgccagt aactcttcgc cattagactc tctcaatccg cgaaaaatat ttatttccgc
      481 cagcggtgta catgatcact ttggcgtcag ctggtttaat cccgaagatc ttgccactaa
      541 gcgtaaagcg atggcccgtg gactacggaa aattttgctc gcccgccacg ccttgtttga
      601 tgaagtagcc tctgccagcc tcgcaccgct ctctgcattt gatgttctga ttagcgatcg
      661 tccgttaccg gcagattatg ttacgcactg ccggaatgct tctgtaaaga tcattacacc
      721 tgattcagaa gacgaatgac ttactgaaaa aacaccgtac tcttgttaaa catcgtcgga
      781 ttggactgat tacgttgcac tttcatcaca tattccagct tatcaatttg gcttatcatc
      841 tactccagac gctggtcatc attgaccaat agtctgatat ggcttttgtc gcggtcctga
      901 atcggcaggc agaaaatgac ttcaacgtta aaagggcgac gggaaaaagc ccacaaaggt
      961 gagtcattac gcccggataa taaacaggct cctccctgtt atggagtccg ttgtattgtc
     1021 cataatgtct gggtttttaa tgcacactgg ataacaaccg gacgaatctt caaatatcac
     1081 tggtgctgac agccggtttg aattcatctc acaaccctgc atagagcgaa tctcctgcct
     1141 gcgcgtcact ccactccatg gtatcaactt cacactcttt atctgcggct agtttcagtc
     1201 accagataag catcgactat gaaaggtgag ccatgacaac ataacgttgg taacgctctg
     1261 atgccttaac ggaagatgcc tgccaccaca gggaatgtaa acgactgaag tgtggccttt
     1321 aatgccgtga acggctcatg gtctcctggc acggttgccg ccccaacctg taacaacatt
     1381 ccacagtaca atgtctgtca gagccggagc ctcccatgcc tgttgtagta actctaccag
     1441 ttacgaacat ccttcctcaa aagtgttgtc gtatctcgca tggaagaaaa gatcctgact
     1501 aagcagcaac acacaacgta ttgcggaacc tgcatgtttt tcctgtaact agtgtattac
     1561 gacatatggt aatagctacc tgtgtggttt cgctggataa caaggggatt tattcgcaag
     1621 taaaatgcct gataaaatac acgaatctag taatcatcaa tatttactct ggtcgaatga
     1681 agtgtgaagt ggattgccag cagatgcggc cagtgatcca ccgcctgctg aacaaaacgc
     1741 cggatttccc ccggctctga aagtaaggct tcggttattt gcactatttt atctctgttg
     1801 aatttggtta agtcggtgca gactcatcaa cacaagtacg gttcgatgca aacagctgtg
     1861 actggcaata tgaaaggaat gatgaatcag tcaggatgac aaagtgccgg ctgaccggag
     1921 gggacgcagg aagattcacg gggggaccag caccagggaa cagcgccaca tcccgggtgg
     1981 tactgctttg atgcgcccgg tgacacaaag cccggattgt tccagacatc ctgaatcaaa
     2041 cgtcccagat taggggcgtc gaaatatgcc tctctgacca ttatattccg gcgtacaggt
     2101 agcaggtcag aagtgacaat gcgtcacctg acgttaaaag tcactacacc caagatgacg
     2161 ttcaacagca ccatgcgatt caatgtaagc ccgggctgtc tgttccagta caccaggctc
     2221 agcgttgtat gtgttagctg catcaaatac caacgacagc acttcaggat acacaaccag
     2281 atgtgtaatg gagttatctt cacccaatac ttttccccac gcctgctcaa tcagatttct
     2341 gagaaccacc acctcacgac tcttacacca gacatcgtta t
//
LOCUS       DADSZD010000308         2380 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.310, whole genome shotgun sequence.
ACCESSION   DADSZD010000308 DADSZD010000000
VERSION     DADSZD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.310"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            59..892
                     /gene="xapA"
                     /locus_tag="J1Z28_004442"
     CDS             59..892
                     /gene="xapA"
                     /locus_tag="J1Z28_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HBA7719193.1"
                     /translation="MYQAQFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            953..2209
                     /gene="xapB"
                     /locus_tag="J1Z28_004443"
     CDS             953..2209
                     /gene="xapB"
                     /locus_tag="J1Z28_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HBA7719194.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            2262..>2380
                     /locus_tag="J1Z28_004444"
     CDS             2262..>2380
                     /locus_tag="J1Z28_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HBA7719195.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVF"
BASE COUNT          501 a          516 c          638 g          725 t
ORIGIN      
        1 gtagggcaat ctcgttataa aaaggcgtta gattccaccc tacaaaaaaa ggatatatat
       61 gtatcaggct cagttttctc ataacccact gtattgcgta gatattatca agacttataa
      121 acctgatttc acgccacgag tggcctttat tttaggttcc gggctgggcg cgctggccga
      181 tcagattgag aacgcggtcg caatttccta cgaaaagctg cctgggttcc cggtaagtac
      241 cgtacacggt catgcgggtg agctggtgct gggttatctc cagggggtgc cagtggcgtg
      301 tatgaaaggt cgcggacatt tctacgaagg tcgtgggatg accatcatga cggatgcaat
      361 ccgtaccttt aagttgctgg gctgcgagtt gctgttctgc accaatgcgg ctggctcact
      421 gcgccctgaa gtgggggccg gcagtctggt cgcattgaaa gatcacatca acaccatgcc
      481 gggaacgccg atggtgggtc ttaatgatga acgttttggt gagcgcttct tctcgctggc
      541 gaatgcctac gatgcggaat accgcgcact gttacaaaaa gtggcgaaag aagaggggtt
      601 ccctctgacg gagggcgtgt tcgtctcgta tccggggccg aatttcgaga ctgcggcgga
      661 aattcgcatg atgcaaatta ttggtgggga tgttgttggt atgtctgtgg tgcctgaggt
      721 tatttcagct cgccattgcg aacttaaagt cgttgcggtc tctgcgatta ccaacatggc
      781 ggaaggtctg agtgacgtga agctttctca tgcccaaacg ctggcagcag cggaactctc
      841 aaagcaaaac tttattaatc ttatttgcgg ctttctgcgc aaaattgcct gagtaaaaca
      901 acaataaacg gccttactga gtgaggccat atgatgcgac aaggaaacga ttatgagcat
      961 cgctttgcgc ttaaaggtaa tgtccttttt gcaatatttt atctggggga gctggctggt
     1021 taccctcggt tcttacatga ttaataccct tcatttcacc ggcgctaatg ttggcatggt
     1081 ttacagttcc aaagggatcg ccgcgattat tatgcctggt ataatgggga tcatcgcaga
     1141 caaatggctg cgtgcagaac gtgcatacat gctgtgtcac ctggtgtgtg cgggcgtact
     1201 tttttatgcg gcatccgtaa ctgatccgga tatgatgttt tgggtgatgt tagtcaatgc
     1261 gatggcgttt atgccgacta ttgcgttatc gaacagcgtc tcttattcct gtcttgccca
     1321 ggcagggctt gacccggtga ccgctttccc gcccattcgc gtttttggta cggtggggtt
     1381 cattgtcgcc atgtgggcag taagcttgct gcatctggaa ttgagtagtc tgcaactgta
     1441 tatcgcgtcc ggtgcgtcat tgctgctgtc ggcttatgcg ctgactttgc cgaagattcc
     1501 ggttgcggag aaaaaagcga ctacatcgct tgccagcaag ctgggcctgg atgccttcgt
     1561 gctgtttaaa aatccacgca tggctatctt tttcctcttc gccatgatgc tgggtgcggt
     1621 gctgcaaatt accaacgttt ttggcaaccc gttcctgcat gatttcgccc gtaatcctga
     1681 gtttgctgat agcttcgtgg tgaaataccc gtccatttta ctgtcagttt cacagatggc
     1741 tgaagtcggt ttcattctga cgataccgtt ctttttgaag cgtttcggta ttaaaaccgt
     1801 catgctgatg agtatggtgg cgtggacgct gcgctttggc ttctttgcct atggcgatcc
     1861 atcaccaacc gggtttattt tgctgttgct gtcgatgatt gtttacggtt gtgcattcga
     1921 tttcttcaat atttctggtt cggtctttgt cgaacaggaa gttgattcca gcattcgtgc
     1981 cagcgcccag ggccttttta tgacgatggt aaatggcgtc ggcgcatggg ttggctcgat
     2041 tctgagcggt atggcagtgg attacttctc ggtggatggc gtaaaagatt ggcataccat
     2101 ttggctgatg tttgcagggt atgctctttt cctcgcagtg atatttttct ttgggtttaa
     2161 atataaccat gaccctgaaa agataacgca tcgttcagtt acacattaaa aggattcgca
     2221 gctctgttcc ttggggctgc ttttatgata aaggttaatt agtgaaagat attttatttt
     2281 cttctggcgt tggttttggc attggtgctt tgtttacaat cgtgcgatta cctattcctg
     2341 ttccaaatgt attgccgggt atattatcca tcgtgtttat
//
LOCUS       DADSZD010000309         2371 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.311, whole genome shotgun sequence.
ACCESSION   DADSZD010000309 DADSZD010000000
VERSION     DADSZD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.311"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>2371
                     /locus_tag="J1Z28_004445"
     CDS             <1..>2371
                     /locus_tag="J1Z28_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBA7719196.1"
                     /translation="SRFSRAGYIMPGQYQMEIRVNGQDISPSAFQIAFLEPPFSDSDN
                     EKPLPEPCLTPEIVSRMGLTEASQEKVTYWNNGQCADFRQLSGVEIRPNPAEGMLYIN
                     MPQAWLEYSDASWLPPSRWDNGIPGLLFDYNINGTVNKPHQGKQSQSLNYNGTAGANF
                     GAWRLRADYQGNLNHTTGSAQGTDSQFTWSRFYMYRAIPRWRANLTLGENYINSEIFS
                     SWRYTGASLESDDRMLPPKLRGYAPQVSGIADTNARVVISQQGRILYDSTVPAGPFTI
                     QDLDSSVRGRLDVEVIEQDGRKKTFQVDTAYVPYLTRPGQVRYKLVSGRSRTYEHTME
                     GPVFAAGEASWGISNTWSLYGGSIVAGDYNALAVGLGRDLSKFGTVSADVTQSVARIP
                     GYDTKQGKSWRLSYSKRFDEVNTDITFAGYRFSERNYMTMDQYLNARYRNDFTGREKE
                     LYTVTLNKNFEDWKASVNLQYSHQTYWDRRTSDYYTLSVNRYFDAFSFKNIALGISAS
                     RSKYLNRDNDSAFVRLSVPWGTGTASYSGSMSNDRYTNTVGYSDTLNNGLSSYSLNAG
                     VNSGGGQPSQRQMSAYYNHNGSLTNLSASFSAVENGYSSFGMSASGGATVTMKGAALH
                     AGGMNGGTRLLVDTDGVGGVPVDGGRVYTNRWGIGVVTDVSSYYRNTTSVDLNKLPED
                     MEATRSVVESVLTEGAIGYREFEVLKGSRLFAVLRMSDNSYPPFGASVTNAKGRELGM
                     VADSGLAWLSGVNPGETLNVGWDGRTQCVVDIPAHPDPAQQLLLPCRQV"
BASE COUNT          538 a          601 c          713 g          519 t
ORIGIN      
        1 tttcccggtt ctcccgggcc ggctatatta tgcccggaca gtatcagatg gaaataaggg
       61 tgaatggtca ggatatttca ccatcggcat ttcagattgc gtttcttgaa ccaccttttt
      121 cggattcgga taacgagaaa ccgttaccag aaccctgcct gacaccggag atagtcagcc
      181 ggatgggact gactgaggcg tcacaggaaa aagttacgta ctggaataac ggacaatgtg
      241 cggatttccg acagttatcc ggagtggaga tacgcccgaa tccggcagaa gggatgctgt
      301 acatcaatat gccacaggcc tggctggaat attccgatgc ctcctggctg ccgccgtccc
      361 gctgggataa cggtattccc ggactgttgt ttgactacaa catcaacggg acagtgaata
      421 agccgcatca ggggaaacag tcccagtcgc tgaactataa cggaactgcg ggggctaact
      481 tcggtgcctg gcgtctgcgt gcggactatc agggcaacct gaatcacacc accggcagtg
      541 cgcaggggac ggacagccag ttcacctgga gccgtttcta catgtaccgc gccatcccgc
      601 gctggcgcgc aaatctcaca ctgggtgaga actacatcaa ttctgagatt ttcagctcct
      661 ggcgctacac tggtgccagt ctggaaagtg atgaccgcat gctgccgcca aaactgcgcg
      721 gttatgcacc gcaggtctca gggattgcgg atacgaatgc gcgtgtcgtg atttcgcagc
      781 agggacgcat actctatgac tccactgtgc cggcaggtcc gttcaccatt caggacctgg
      841 acagctccgt gcgtggccgc ctggatgttg aagttatcga gcaggacggg cggaaaaaaa
      901 cgttccaggt ggacacggca tatgtgccgt atctgacccg tccgggccag gttcgttaca
      961 agctggtttc cggtcgttcg cgtacctatg agcacaccat ggagggtccg gtctttgctg
     1021 ccggtgaggc atcctggggt atcagtaaca cgtggtcgct ttatggaggc agtattgttg
     1081 ccggggatta taacgctctg gctgtggggc tgggccggga cctgagtaag ttcggcactg
     1141 tttccgctga cgtaacccag tccgtggccc gtataccggg ttatgacaca aaacagggaa
     1201 aatcctggcg tctgagctat tccaaacgtt ttgatgaagt gaatacggat atcacttttg
     1261 cgggttaccg cttctctgaa cgcaattaca tgacgatgga ccagtacctc aatgcccgtt
     1321 accggaatga ttttacggga cgtgagaagg agctgtacac cgtcacactc aacaagaact
     1381 tcgaggactg gaaggcatcg gtcaatctgc agtacagcca tcagacttac tgggaccgcc
     1441 ggacatctga ctattacacg ctgtcggtga accgctactt cgatgcgttc agctttaaga
     1501 atatcgcgct ggggatatct gcatcccgtt cgaaatacct gaaccgggat aacgattcgg
     1561 cgtttgttcg tctgtccgtg ccgtggggaa ccggtacggc cagctacagc ggcagcatga
     1621 gcaacgaccg ttacaccaat acggtgggtt acagcgacac actgaataac ggcctgagca
     1681 gttacagcct gaatgccggc gtgaacagtg gcggtggtca gccgtcacaa cggcaaatga
     1741 gtgcctatta caaccataac ggttccctga caaatctgtc agccagtttc tctgccgtgg
     1801 agaacggata cagctcattc gggatgagtg catcgggggg ggcgacggtc acgatgaaag
     1861 gagccgcgtt acatgccgga ggaatgaacg gaggaacccg tcttctggtg gataccgatg
     1921 gtgtcggagg tgtgccggtt gacggtggtc gggtttatac caaccggtgg ggaattggtg
     1981 tggtgacgga tgtgagcagt tattaccgca acaccacatc agtagacctg aacaagctgc
     2041 cggaagatat ggaagccacc cgctcggtgg tggagtcggt tctgacggaa ggggccattg
     2101 gttatcgtga gttcgaagtt ctgaaaggta gccgtctgtt tgctgtgttg cggatgtctg
     2161 ataacagcta tccgcccttt ggtgccagcg tgacaaatgc gaaaggccgt gaactgggga
     2221 tggtggcaga cagcggtctg gcctggctga gcggagtgaa tccgggagaa accctgaacg
     2281 tcggctggga cggccggaca cagtgtgtgg tggacatccc ggcacatccg gacccggcac
     2341 aacagttgct gctgccctgt cgtcaggtga a
//
LOCUS       DADSZD010000310         2307 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.312, whole genome shotgun sequence.
ACCESSION   DADSZD010000310 DADSZD010000000
VERSION     DADSZD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.312"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..762
                     /gene="nirC"
                     /locus_tag="J1Z28_004446"
     CDS             <1..762
                     /gene="nirC"
                     /locus_tag="J1Z28_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBA7719197.1"
                     /translation="IARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSVRPL
                     VMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMWAILPQTWLGNLVGSV
                     FVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMALRTE
                     GAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGN
                     TLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            781..2154
                     /gene="cobA"
                     /locus_tag="J1Z28_004447"
     CDS             781..2154
                     /gene="cobA"
                     /locus_tag="J1Z28_004447"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBA7719198.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          504 a          588 c          648 g          567 t
ORIGIN      
        1 attgcacgcc tgtcggcaaa taacccgctc ggcttttggg tcagctccgc catggcgggc
       61 gcgtatgtgg gtcttgggat catcctgatt ttcacgctcg gtaatttgct cgacccgtcc
      121 gtacgtcctc tggtgatggg cgcgaccttt ggtatcgcct taacgctggt gattatcgcc
      181 ggttctgaat tgttcaccgg acacaccatg ttcctcacct ttggggtgaa agcgggcacc
      241 atcagccacg ggcaaatgtg ggcaatcctg ccgcaaacct ggctgggtaa cctggtcggt
      301 tccgtcttcg ttgccatgct ttatagctgg ggcggcggta gcctgctgcc ggtagatacc
      361 agcatcgttc actccgtcgc gctggctaaa accaccgcac cggcaatggt actgttcttc
      421 aaaggtgcat tgtgtaactg gctggtttgc ctggcaatct ggatggcgct gcgcactgaa
      481 ggggcggcga aatttatcgc tatctggtgg tgtttgctgg catttatcgc gtccggctac
      541 gagcactcca tcgctaacat gacgctgttc gcactctcct ggttcggcaa ccacagcgaa
      601 gcctacacgt tggcgggcat tggtcataac ctgctgtggg tgacgctggg taatacttta
      661 tcgggtgccg tattcatggg attgggttat tggtatgcta cgccgaaagc gaatcgtccg
      721 gttgcggaca aatttaatca aactgaaacg gctgccggtt aattactaag gggtttttac
      781 gtggatcatt tgcctatatt ttgccaatta cgcgatcgcg actgtctgat tgtcggcggt
      841 ggtgatgtcg cggaacgcaa agcaaggttg ctgttagacg caggcgctcg cttaacggtg
      901 aatgcattag cgtttattcc acagttcacc gcatgggcag atgcaggcat gttaaccctc
      961 gtcgaagggc catttgatga aagccttctc gacacctgct ggctggcgat tgcagcgaca
     1021 gatgatgacg cgcttaacca gcgcgtcagc gaagccgctg aagctcgtcg catcttctgt
     1081 aacgtggtcg atgcgccgaa agccgccagc tttattatgc cgtcgattat tgaccgctcg
     1141 ccgctcatgg tagcggtctc ctctggcggc acctctccgg ttctggcgcg cctgttgcgc
     1201 gaaaaactcg aatcactgct gccgttacat ctgggccagg tagcgaaata cgccgggcaa
     1261 ttacgcgggc gagtaaaaca gcagttcgcc acgatgggtg agcgtcgccg tttctgggag
     1321 aaattgttcg ttaacgaccg cctggcgcag tcgctggcca acaacgatca gaaagccatt
     1381 actgaaacca ccgaacagtt aatcaatgaa ccgctcgacc atcgcggtga agtggtgctg
     1441 gttggtgcag gtccgggcga tgccgggctg ctgacactga aaggtctgca acaaattcag
     1501 caggcagatg tggtggtcta cgaccgtctg gtttctgacg atattatgaa tctggtacgc
     1561 cgcgatgctg atcgcgtttt cgtcggcaaa cgcgcgggat accactgcgt accgcaggaa
     1621 gagattaacc agatcttgct gcgggaagcg caaaaaggta aacgcgtggt gcgtctgaaa
     1681 ggcggcgatc cgtttatttt cgggcgtggc ggagaagagc tggaaacact gtgcaacgcg
     1741 ggtattccgt tctcggtggt tccgggtatt accgcagctt ctggttgctc tgcctattcg
     1801 ggtattccgc tcacgcatcg cgattatgcc cagagcgtac gcttaattac cggacactta
     1861 aaaaccggtg gcgagctgga ctgggaaaac ctggcggcag aaaaacagac gctggtgttc
     1921 tatatggggc tgaatcaggc cgcgaccatt cagcaaaagc tgattgaaca cggtatgcct
     1981 ggcgaaatgc cggtggcaat tgtcgaaaac ggaacagcag tcacgcagcg cgtgattgac
     2041 ggtacgctca cacagctggg cgaactagcg cagcaaatga acagtccatc gctaattatt
     2101 attggtcggg ttgttggcct gcgcgataaa ttgaactggt tctccaacca ttaatttaac
     2161 cagggccaga gaattctggc ctttttcgtt tacaactctc tttatatatt cagcagataa
     2221 aaacataccc ccttaataaa tattgtctgc taatgggtta tattcaccgt tacagcgact
     2281 tacaaaatta aatcaatcgc tcactga
//
LOCUS       DADSZD010000311         2274 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.313, whole genome shotgun sequence.
ACCESSION   DADSZD010000311 DADSZD010000000
VERSION     DADSZD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.313"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            61..1461
                     /locus_tag="J1Z28_004448"
     CDS             61..1461
                     /locus_tag="J1Z28_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001702216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBA7719199.1"
                     /translation="MTQAPLGLVAASALGAVSLVCQNLIDVCRLNTLRGPVSLFFLTL
                     AESGERKTAVDKLLMKPLYQQEMQLYSRYKSELAVWKNKEELLKAQKKALLSKLNKEL
                     RKGADESETLRQLEVLQKNSAEEPVRYKFIFNDATTAAIKNQLCGKWRSVGIMSDEAG
                     IIFDGYTLSELPFINKMWDGSVLSVDRKNEPEQMIENARMTLSLMVQPGLFDRYMERK
                     GSVARDSGFLARCLISKPATTQGKRFINGAVIPGGSLTAFHERLMELARGSIEKSSED
                     ERYCLHFSPEAQKIFIEHYNVLEQDLSPSGPLSPFRGHVSKKTENIARIAALFQYFSY
                     GEGKISADIMTSAVVISSWYTDEYKKLFALPDESELQQKDAEELFDWLIEECRGECPP
                     RVRKNYILQCGPGRFRNRKKLNALLNILESQFRLSVVPEGKTMYVLLPQIASLKLSDV
                     SGIFTSGYHYNKLRAK"
     gene            complement(1783..2274)
                     /locus_tag="J1Z28_004449"
                     /pseudo
     CDS             complement(1783..2274)
                     /locus_tag="J1Z28_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          599 a          515 c          566 g          594 t
ORIGIN      
        1 gagtttcccc tgagttcgct gcctccactc attcgtgatg ccgttattga agctcagcaa
       61 attacgcagg ctccccttgg gcttgtggca gcgtctgccc tgggcgctgt ttcacttgtc
      121 tgccagaatc tgattgatgt ctgcagactg aatacactgc gggggccggt ctctcttttt
      181 ttcctgacgc ttgccgagtc cggagaacga aaaaccgcag tggacaaatt attaatgaag
      241 cccctgtacc agcaggagat gcagttatat tccagataca aaagtgaact ggctgtctgg
      301 aaaaacaagg aagagctttt aaaggctcag aaaaaagcat tgttgtcaaa actgaataaa
      361 gaactccgca aaggcgcgga tgagtcagaa accctcagac aactggaagt acttcagaaa
      421 aacagtgcgg aagaacctgt gaggtataaa ttcattttta atgatgccac gactgcggca
      481 attaagaatc agctttgtgg taaatggcgt tcagtgggta ttatgtcaga tgaagccggt
      541 attatatttg atggatatac cctgtctgag ttgcctttta ttaacaaaat gtgggatggc
      601 tctgtattgt ctgtggacag aaagaatgaa cccgaacaga tgattgaaaa tgccagaatg
      661 acgttatccc tcatggtgca acccggtctt tttgaccgat atatggagcg aaaaggttct
      721 gttgccaggg acagtggttt tcttgcccgt tgtcttatca gtaaacctgc aaccacgcag
      781 gggaaacgct ttattaacgg agctgtgatt ccgggaggat cattaacggc atttcatgaa
      841 cgattaatgg aactggccag aggcagtatt gaaaaatcca gtgaggatga acggtattgt
      901 ctgcattttt cgcctgaagc gcaaaaaata tttattgagc actataatgt tctggagcag
      961 gatttatccc cttccggacc cttaagcccg tttcggggac atgtttcaaa aaaaacagaa
     1021 aacattgcaa gaattgcagc gttatttcag tatttcagct atggtgaagg gaaaatctct
     1081 gccgatatca tgacgtctgc tgtcgtaata agttcgtggt acacagatga atataaaaaa
     1141 ttatttgcgt tacctgatga atctgaactg cagcagaagg acgccgagga gctgttcgac
     1201 tggctcattg aggagtgccg cggagaatgc ccgccccgtg tcaggaaaaa ttatattctc
     1261 cagtgtggcc cgggccggtt cagaaacagg aaaaaactga acgcattgct gaacattctt
     1321 gaatctcagt tcaggctttc tgtcgtgccg gaaggaaaaa ccatgtatgt tctgcttccc
     1381 cagatagcca gtttaaagtt gtctgatgtc tctggtattt ttacctcggg ttatcactac
     1441 aataagctga gagcgaagta accggtgagc agtttttctc cattatgctt cagacttaga
     1501 tgattgatta ttattgggat ttcatcaccg atattcagat gtatgaatat cggtgatgaa
     1561 ctaaagctca ggagctgcgt ggtggtgatg aatcaacagg catataactg atacccgccg
     1621 gtcagaagtt ccagcctgcg actttcctgt ctgcgtgccc gcagtccggc gttcaggtgc
     1681 agcgtgtgac agaacgcacg gagagcgaaa cggcaggcag acaggccgtt cagtcagccc
     1741 tggcaccctg cccggatacc agcttccctg aaacaactgg catcaggtgc gcttatcgtc
     1801 aaccgttgtg cggtatgcca ggagcacgta ctgatccatc agatccgtca gtgctgcgct
     1861 ctgcttctga cggttgatga tccattcctc cagttctttt tctgatttcc gtttcagatt
     1921 ctccgtccag tcgtcaaggc agtttccggt cattaaatca agccagcaaa gccagaccag
     1981 tttgaggcgc aactccgtgc caccaaactg acggaagagg ctgatcagtt tatttgccag
     2041 cgaacgctgc ctcagacgtt cacattcaga caggagaatg ccttccagca tcggatgatg
     2101 aaccagcaga tgttcactgg tgcataaatt cactatcaga tcatcatcca ggggcggcgt
     2161 ggtccgtttg tccggaccat aaatcagtcg gtgcagactc tggggtacga tggcaatctc
     2221 cggtttttca ccattgaatg acaccccccc cgggcgtcag attatggaag tagt
//
LOCUS       DADSZD010000312         2265 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.314, whole genome shotgun sequence.
ACCESSION   DADSZD010000312 DADSZD010000000
VERSION     DADSZD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.314"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            25..342
                     /locus_tag="J1Z28_004450"
     CDS             25..342
                     /locus_tag="J1Z28_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719200.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQCKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(672..2033)
                     /locus_tag="J1Z28_004451"
     CDS             complement(672..2033)
                     /locus_tag="J1Z28_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBA7719201.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
BASE COUNT          562 a          519 c          603 g          581 t
ORIGIN      
        1 tggtaattaa tgggggaagg taaggtgaaa aagatagctg ctatatcatt aattagtgtt
       61 tttcttatgt ctggttgtgc tgtgcataat gatgagacaa gtatcggtaa atttggcctt
      121 gcatataaaa gtaatattca gtgtaaactc gataaccaat actacaccga agccgaagct
      181 tctttagcca ggggtagaat atctggtgca gaaaatatag taaaaaatga tgcaactcat
      241 ttctgtgtta ctcagggcaa aaaaatgcag atagttgacc tgaagacaga aggtgcagga
      301 ttacatggcg tcgctcgtct gacattcaaa tgtggagagt gagaatattt tttggtaagc
      361 gtcaaacatg cgcgttctgg ctgtgcgtaa ccggaacctg tgcgagcacg atgtcgataa
      421 gtgaaaggca tcgtgctatg aaggaggatt ctatcgatgt ggtcaatgga agacggtgac
      481 cagggatagg gcttatgcat aaaaaataag cccgtgtaag ggagattaca caggctaagg
      541 aggtggttcc tggtacagct agcattttat gggttatgtt tttcagcgaa acggatgata
      601 accttaataa atgcagctgt atgtgatcgg tttctaagaa tttttcatcc gggaaaaata
      661 atcgaaatta atcacttacc gtgggggtta cgcgtggttt caccggagaa attacgcatc
      721 agcagcgcgt aatttagctc aagatcctgc ggcaccggga gccacacagt ataaccatcg
      781 cctggtgcga ccggcattgc ttcacctttg gcgttttcca tatgctcaag ggtaaagtta
      841 atgttgcctt gcggcgtcat cagctcaagg ctgtcgccaa cggagaattt atttttcacc
      901 gctaccgccg cgagctcccc cttgcgctca ccggtaaact caccaacaaa ctgctggcgg
      961 tcagaaaccg aataaccgta ttcgtagttc tgataatcgt cgtgggtatg acgacgcagg
     1021 aaaccttcgg tatagccacg atgcgccaga ccttccagcg tttccagcag gctggtatcg
     1081 aacggtttgc ccgcagcggc gtcatcgata gctttacggt aaacctgcgc ggtgcgtgcg
     1141 caatagtaga aagatttggt acggccttcg attttcagcg aatgcacgcc cattttggtc
     1201 aggcgttcaa catgggcgat ggcgcgcaga tctttcgagt tcatgatgta agtgccgtgc
     1261 tcatcttcaa acgcggtcat atactcgccc ggacgctggg cttcttcgat cataaacact
     1321 ttgtcggttg gcgcgccgat acccagcgtc ggctcaacat tttgcaccgg aatcggctcg
     1381 tacttgtgta cgatgttgcc gacgtcatct tctttccctt cctggacgtt gtactcccag
     1441 cggcaggcgt tggtgcaggt gccctggttc gggtcgcgct tgttgatata gccagagagc
     1501 aggcagcgac cggagtaggc catgcacagc gcgccgtgaa cgaagatttc gatctccata
     1561 tccggcacct gattgcggat ctcttcaatc tcttccagtg acagttcgcg agagaggatc
     1621 acgcgggtca gacccatttg ctgccagaat ttcaccgtcg cccagtttac ggcgttagcc
     1681 tgtaccgaga ggtggattgg catttccggg aagtgttcac gcaccagcat aatcagccct
     1741 ggatcggaca taatcagcgc atccggcccc atttccacca ccggtttcag gtcacggata
     1801 aaggttttca gcttggcgtt gtgcggtgca atgttgacca cgacataaaa ctttttcccc
     1861 agcgcgtggg cttcattgat gccgagctga agattttcgt ggttgaattc gttgttgcgc
     1921 acacgcaggg agtaacgcgg ctggcccgca taaacagcat ctgcgccata agcgaaagcg
     1981 taacgcatat ttttcagcgt tcccgccggg gaaaggagtt ccggtttaaa cataattctc
     2041 tcgttctgat gacaggtcag agccgcttca cctgatgaag cggttagggg agtgccccca
     2101 ctttaagggc gggcattgta gcgcaatcgg cgggtttagc tccagtgtaa atgtgagggg
     2161 agaagtggca gagatgggag tatctacacg aaatcttcat gtagtgataa aagaaaaagt
     2221 tgacgatact gactactttc ctgaatgttc atcccctgat aatta
//
LOCUS       DADSZD010000313         2220 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.315, whole genome shotgun sequence.
ACCESSION   DADSZD010000313 DADSZD010000000
VERSION     DADSZD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.315"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            226..987
                     /locus_tag="J1Z28_004452"
     CDS             226..987
                     /locus_tag="J1Z28_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719202.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            1008..>2220
                     /locus_tag="J1Z28_004453"
     CDS             1008..>2220
                     /locus_tag="J1Z28_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HBA7719203.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAVGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKRE"
BASE COUNT          637 a          436 c          524 g          623 t
ORIGIN      
        1 agttcatcat gaaaaattcc tataaatcaa agggtgatgt ttaaaacaga cacattttct
       61 gaaacacgat atccctccgc ccgttcgcaa aagtaatctg cgatcttcat cacgcttcga
      121 tagtcatctt taaatatact ggcctggtca ttcttaataa aaatgaattg tctggtgtat
      181 taattcctat ggaatcataa aaacaatgaa tggagaacat attatatgtc tgccaaaaga
      241 cgacttctta ttgcgtgtac cctgataaca gctatctatc attttcctgc atattcttca
      301 ttagaatata aaggaacctt tggttcaata aatgcgggtt atgcagactg gaatagtgga
      361 tttgtaaaca ctcaccgtgg tgaagtatgg aaagtgactg cggattttgg ggtaaatttt
      421 aaagaagcag aattttactc attttatgaa agtaatgtac tcaatcatgc tgtagcaggg
      481 agaaatcata cggtttcagc aatgacgcat gtcagactct ttgactctga tatgacattc
      541 tttggcaaaa tttatggcca atgggataac tcatggggtg acgatctgga catgttttat
      601 ggattcggtt acctcggctg gaacggcgag tggggctttt ttaaaccgta tattggattg
      661 cataatcaat ctggtgacta cgtatcagct aaatatggtc aaacgaatgg ttggaatggt
      721 tatgttgttg gctggacagc agtattacca tttacgttat ttgacgaaaa atttgtttta
      781 tctaactgga atgaaataga actggacagg aacgatgctt acacggagca gcaatttggc
      841 cggaacgggt taaatggcgg tttaactatt gcctggaagt tctatcctcg ctggaaagcc
      901 agtgtgacgt ggcgttattt cgataataag ctgggctacg atggctttgg cgatcaaatg
      961 atttatatgc ttggttatga tttctaattt tttatcaggg aataattatg cagaaaacgt
     1021 taatggccag tttgatcggc cttgcagttt gcacagggaa tgcttttagt cctgccttag
     1081 ccgcagaggc taaacaacct aatttagtca ttattatggc ggatgattta ggttatggcg
     1141 atttagcaac atatggtcat cagatcgtta aaacacctaa tatcgacagg cttgcccagg
     1201 aaggggtcaa atttactgac tactatgccc ccgctccttt aagttcacct tcacgcgcag
     1261 ggctattaac cggccggatg ccatttcgta ctggaattcg ctcatggatt ccttcaggca
     1321 aagatgttgc cttagggcgt aacgaactca cgattgctaa tctactcaaa gcgcaagggt
     1381 acgacacggc aatgatgggt aagctgcatc tgaatgcagg cggcgatcgc accgatcagc
     1441 cacaagcaca agatatgggc tttgattact cactggttaa tacggcgggc ttcgttaccg
     1501 acgccacgct ggataacgct aaagaacgcc cgcgttatgg catggtttac ccgacaggct
     1561 ggctacgtaa cgggcaaccc actccacgag ccgataaaat gagcggtgag tatgtcagtt
     1621 cggaagtcgt caactggctg gataacaaaa aggacagcaa gcctttcttc ctctatgttg
     1681 cttttaccga agtgcatagc cccctggctt cgcccaaaaa atacctcgat atgtactcac
     1741 aatatatgag cgcgtatcag aagcagcatc ctgatttatt ttatggcgac tgggcagaca
     1801 aaccctggcg tggtgtgggg gaatattatg ccaatatcag ctatctggat gcacaggttg
     1861 gaaaagtgct ggataaaatc aaagctgtgg gtgaagaaga taacacaatc gttattttta
     1921 ccagtgataa cggtccggta acgcgtgaag cgcgcaaagt gtatgagctg aatttggcag
     1981 gggaaacgga tggattacgc ggtcgcaagg ataacctttg ggaaggcgga attcgtgttc
     2041 cagccattat taaatatggt aaacatctac cacagggaat ggtttcagat acacccgttt
     2101 atggtctgga ctggatgcct actttagcga aaatgatgaa cttcaaatta cctacagacc
     2161 gtactttcga tggtgaatcg ctggttcctg ttcttgagca aaaagcattg aaacgcgaaa
//
LOCUS       DADSZD010000314         2185 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.316, whole genome shotgun sequence.
ACCESSION   DADSZD010000314 DADSZD010000000
VERSION     DADSZD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.316"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..897
                     /locus_tag="J1Z28_004454"
     CDS             <1..897
                     /locus_tag="J1Z28_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7719204.1"
                     /translation="NSPNGRLYSTVGVPVPINISGTSNASLSYRECNGFTWSHGLFWS
                     EYFAWMVIPKSIVYNGVVIYLDIQSKGGWSLDAEDNDNYYFVQGYAWDEVNTSGNTCA
                     FSGDNRPLSWTFGGVTMSAYLPADMPLGMHTIPIKFLRGIQRNNFSYIGGRYKIPSST
                     MKTFTFRSVSNLNFEVIGGCRPSVQSLDIEHGSLSIDNANGHMVHKSLSVYCDIPASI
                     NIYLLPNTPGNYSSTGTNVGLGNGWDSIVSLDGVERDKEYIRWSSAGSKTITVGSRLY
                     GEAGKIKPGNLSGSMTMVFSIR"
     gene            938..1939
                     /locus_tag="J1Z28_004455"
                     /pseudo
     CDS             938..1939
                     /locus_tag="J1Z28_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          606 a          414 c          547 g          618 t
ORIGIN      
        1 aatagtccca atgggcggct ttattcgaca gtcggtgtac cagttccaat taatataagc
       61 gggacgtcaa atgcaagcct gagttacaga gagtgtaatg gttttacatg gtcacatggg
      121 ctgttctggt cagaatattt cgcatggatg gttataccca agagtattgt atataacggg
      181 gtagtgattt atctggacat acagagcaaa ggaggctggt cattggatgc ggaggataat
      241 gataactatt actttgtgca gggttatgca tgggatgagg tgaacacttc tggtaataca
      301 tgtgcatttt ctggcgacaa caggccttta tcatggacat ttggtggtgt aactatgagc
      361 gcatatcttc ctgctgatat gccattagga atgcatacga tccccattaa atttctacgt
      421 ggtatacaac gaaataactt ctcttatatt ggtggacgtt ataagatacc atcatccacg
      481 atgaaaactt ttacattcag aagtgtgtca aatttaaatt ttgaagttat tggtgggtgt
      541 cgtccctcgg tacagtcttt agatatcgag catggtagtc tctcaattga taatgctaat
      601 ggtcatatgg tacataagtc tctctctgtt tactgtgata ttcctgccag cattaatatt
      661 tatttattgc caaatacacc cgggaattac agttctacag gtacaaatgt cggcctcgga
      721 aatggatggg attcaatagt ttctcttgat ggcgtggagc gagacaaaga atacatcaga
      781 tggagcagtg ctggttcaaa aacaattaca gtggggagtc gtttatatgg tgaggccgga
      841 aagataaaac ctggaaacct gtcaggttct atgacaatgg tttttagtat tcgttagtca
      901 aagaagacgg ttttattgtt atatctgcgc cacaaagagg caggaatttc agggtattga
      961 gtccgctggt aatgctatta tccctgaaca acgcgaaatt cagcagctta aagcgcgttg
     1021 agatggaaaa agaaatacta aagcaggctg ccgtgctgat gagcgaaatc cccgggaagc
     1081 tgtcgcgcta atcacacggc tgaaagcaaa gtggccagtg tgggttattt gtcatttatt
     1141 cggtattaac cgtagcgttt attacgcgca ggtgacgcgt cctgttaatg tgcaaagaat
     1201 tgacttacga agtcgggtga gggctttcca tgctctcagt cgtggcgcag ccggtagccg
     1261 ggcaatcagt cagatgttgc gccagagtag cgttgatgca ggccggtggc tggcacgacg
     1321 actgatgcgg gaatgcgggc tgacaagtcg acagatggtt aaacatcact accggatcaa
     1381 cgaagacaaa agtccgccat tgccaaattt actgaaccgg caatttcacc ccgccgcacc
     1441 agaccgcgtc tggtgcggcg acatcagttt tattcgcctg catgacaaat ggtgttagct
     1501 cgcgctcgtt gttgatttat attcacggcg gattatcggt tcagccctct cattaaccgc
     1561 tgatgctgag ttggtgtgta gggctttgcg taatgcactg gaaacacgat cacgtgatgg
     1621 ccgtctgctg tttcattcag atcaaggtgt tcagtataaa agcaataaat acagaaagtt
     1681 actctggcgt tatggggtaa tgcaaagtat gagttgcaga gggaatggcc tggataattc
     1741 gccgatggaa agagtctttc gcagccttaa aaatgaatgg cttccgaaag gtggttatgg
     1801 tgattttatc catgcggtac gtgatataaa ccagtggatt aatggttatt acaacgttta
     1861 tcgacctcac acgaacaacg atggtttacc gccttgctta catgaagaaa agtggaaaca
     1921 ggttattccg gtgtcctgat tttgtgaacc actacaataa tcctttttca cagacttgtc
     1981 agcagccagc atttatgttc ttttatctgc gggaatttat ccgtctgcct gtcgggacta
     2041 ctctgtcata cagaaatcag gtaggaataa attgtagtgg aaagtcgatg tttaccggat
     2101 gactgatgcg cgcttgtaca cagacagtgt gtttcagtaa tatggagaat aatgaaatga
     2161 ataacacaga cacattagaa aaaat
//
LOCUS       DADSZD010000315         2176 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.317, whole genome shotgun sequence.
ACCESSION   DADSZD010000315 DADSZD010000000
VERSION     DADSZD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.317"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            30..659
                     /locus_tag="J1Z28_004456"
     CDS             30..659
                     /locus_tag="J1Z28_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBA7719205.1"
                     /translation="MCEHLITVVEKYGAAERVHLGKQAGNVGRAVTKLPLMGKSLHKT
                     IERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGA
                     YDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRE
                     SLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEKAITDFMTARGYH"
     gene            complement(708..>2176)
                     /gene="pitB"
                     /locus_tag="J1Z28_004457"
     CDS             complement(708..>2176)
                     /gene="pitB"
                     /locus_tag="J1Z28_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBA7719206.1"
                     /translation="TGLLLLLALAFVLFYEAINGFHDTANAVATVIYTRAMQPQLAVV
                     MAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWF
                     FGLPASSSHTLIGAIIGIGLTNALLNGSSVMDALNLREVTKIFSSLIVSPIVGLVIAG
                     GLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGAND
                     GQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLITM
                     EPPLPATSTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIML
                     CISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWR
                     RVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVD
                     GGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
BASE COUNT          573 a          574 c          540 g          489 t
ORIGIN      
        1 ttggtagcga tggttcaggc aagtcaacgg tgtgtgaaca tcttattacc gttgtcgaaa
       61 aatatggtgc tgccgaaagg gttcatttag gaaaacaggc cggaaatgtc ggtcgtgcag
      121 tgacaaaatt accgttgatg ggaaaatcct tacataaaac aattgaacga aatcaggtaa
      181 aaacagcaaa aaaattgcct ggaccagttc cggcgctggt aattacagcg tttgtcgccc
      241 gccgcttact gcgctttcgt catatgcttg cctgtcgtcg tcgcgggtta attgttttaa
      301 ccgaccgtta tccgcaggac cagattcctg gcgcttatga tggtacggtg tttccgccta
      361 acgttgaagg tggtcgtttt gtctcatggc tggcaagtca ggaacgtaaa gcatttcact
      421 ggatggcgag ccacaagccc gatctggtca tcaaacttaa tgttgacctt gaagttgcct
      481 gcgcgcgtaa acccgaccat aaacgggaat cgctggcgag gaagattgcc ataacgccac
      541 agttaacctt tggtggtgca caactggttg atatcgacgc caatcagcca ctggaaaagg
      601 tgctggttga tgcagaaaaa gcgattacgg attttatgac cgcgcgtggt tatcactagt
      661 gaaaaatgga aatgcccgat cgccaggacc gggcattttc aggaaggtta aatcaactgc
      721 aatgctatcc agtacaaccc accagaaaga aaaatcgccg ccggtaaagt aaacacccac
      781 gccatcagga tgctggttac cgttttacgc tgtaacccac cgccgtccac caccatcgtc
      841 cctgctactg cagacgagag tacgtgtgtt gtggagacgg gcatcccaat ataactggca
      901 aggccgatag acactgccgc cgtcatttgt gccgccatgc cttgcgcata cgtcatgccg
      961 cgcttaccaa tcttctcacc gatggtcatc gctacacgac gccagccaat catggtgccg
     1021 atgccgagcg ccagtgctac cgccatgatg atccataccg gtgcgtactc aatggtgctt
     1081 aacatatcgc tgcgcagttt tttcagcagg ttctggtctt ctttactgac gcctggcagt
     1141 ttcgccagct tcgcggaggt atcagagatg cacagcataa tgcggcgcag ctggctgcgc
     1201 tgactcacgc ttaacggctc gtaactttcc atattgcctg gcagcatcgt tttaacgcgc
     1261 gcaatcgcat caaaggtatt tgccggatga cagtgaaact ctgttacttg cgtgccatca
     1321 gtcgatgtcg caggcaatgg aggttccatc gtaatcaact tctgcggcag ttcaggatgc
     1381 tgttgcaggt agtgttcgaa gttggtgacg gcatcgcggg tacgggtaat ttcatagccg
     1441 gacgcattca tattgacgac aaacccggcg ggagcaatcc ccaccagcac cagcattacc
     1501 aggccgatcc ctttctgccc gtcgttagcg ccgtgcgaaa acgccacgcc cgcagcggaa
     1561 acaatcagcg caatacgcgt ccagaatggc ggcttacgtt tgcctttttt ctttttgcga
     1621 tcttccggaa tgcggtgaat acggtcacgc tttttcgtcc cgctccagaa acggcgtagc
     1681 agaaaaatta gcccccccgc aatcaccagc cccacgatgg gagagacaat gagtgaagag
     1741 aaaatcttgg tgacttcgcg caggtttaac gcatccatca ccgacgaacc atttaacagc
     1801 gcgttggtta aaccaatgcc gataatcgca ccaatcaaag tgtgcgaact ggaggccggt
     1861 aaaccaaaga accacgttcc cagattccag ataatcgccg ccagcagcat ggaaaagacc
     1921 atcgccagac cgtgagttga ccccatattc agcaacaaat cggttggcaa catatggacg
     1981 atggcatagg caacgctaag cccgccaagc aatacgccaa aaaagttaaa aaatgccgcc
     2041 atgaccacag caagctgtgg ttgcatggca cgggtataaa taacggttgc caccgcattc
     2101 gccgtgtcat gaaaaccatt gattgcttca tagaacaaaa caaacgccag agcaagtaat
     2161 aacaaaagcc ccgtat
//
LOCUS       DADSZD010000316         2154 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.318, whole genome shotgun sequence.
ACCESSION   DADSZD010000316 DADSZD010000000
VERSION     DADSZD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.318"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(84..809)
                     /gene="fimC"
                     /locus_tag="J1Z28_004458"
     CDS             complement(84..809)
                     /gene="fimC"
                     /locus_tag="J1Z28_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBA7719207.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESAVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(846..1385)
                     /locus_tag="J1Z28_004459"
     CDS             complement(846..1385)
                     /locus_tag="J1Z28_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7719208.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAEACR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSSPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(1450..1998)
                     /gene="fimA"
                     /locus_tag="J1Z28_004460"
     CDS             complement(1450..1998)
                     /gene="fimA"
                     /locus_tag="J1Z28_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/fibrin-binding adhesin FimA"
                     /protein_id="HBA7719209.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AAHTNVLALQSSAAGSATNVGVQILDRTGVALTLDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT          549 a          561 c          460 g          584 t
ORIGIN      
        1 taaattcaga tatgacatta ttcctccggt ttaaatcacc cggcagtcaa ttctttttat
       61 tcaggcgaaa aaatccccct gcgttattcc attacgcccg tcattttggg ggtaagtgcg
      121 ccataatcat ttattgttcg gtaagtaata ttgcttcctg catcagaagg caatttaacc
      181 gcgctttcgc ccattggagg caccaatgca ttttcaagaa cccgagttcc ggcattcaac
      241 tctgttaccg tcaggtaata gggtgtcggg ttgatcagcg tcagagaatt cgcgctacga
      301 cgaaatctta atttttctgc ggcctgatcg ggtggcaacg ctaatttagc cggacgatag
      361 tacagtttaa tgcggctgat aattgcgagc tgtagcgtat tctcagtcaa ttttgattta
      421 tccattgacg gaatcgcttt aacattcatc cagaataaac tttcccgatc ctgtggcaat
      481 tggttatttg ttgcatcaag aatacgcaag gtattctctt tttttccctt catcgcaaac
      541 agaggtggcg tcacgataaa acggccatcc tttacaccat cggcattttc tacccatgat
      601 tgaattaaat aggtgctatt ttcatcatta tttgtcacgg caagttgcac ttgtttttgc
      661 cctgccggat aaattacgcg agtcgcacct aaggccactc ccgcttcagc gcgtccggca
      721 accatcattg ccatgaacat caggatacct gccagcaagc agaatgttat ttcctgcgat
      781 ttccttacat tgacgttttt attactcact gtcctgttcc tgttatcaca ttatctgctg
      841 aacaattact gataggttaa agagaaccag gcctgggcat tggcgatgcc gccagtaacc
      901 cgacgcccgg tagcacgata tttggcgatg aaatgtagcg aagtagagcc tgagtaaagc
      961 cgtttccagt ttactggagg acgattaatc ggtacgaggt ttccttcatc atcaaacaac
     1021 gctacgccaa tattggtggc tatccctggc ccctctccca cggaaagcac atccggattt
     1081 ttaccatccg cgacaccgtg aaacgccaca cccacacgtt cactcaccac cgtgctacat
     1141 tcccgtaaat gaataacaaa aggcaccggt gagctatctt cccctaccgc atgaaaccgg
     1201 ttactgctga tttgccccat attgaccgtc atttgtttat caccggcttc aatccggcaa
     1261 gcttccgcaa taatgacgcc ctgaaattgc atatttccgc cgggcagcgt ggtattccat
     1321 ttatttccgg ccagagcaaa cattggcagc aacgacgcta acagaaataa tctcttcctt
     1381 ttcatcgtta tttttatcgc acaagggtgg gcatccctgc ccgtaatgac gtccctgaac
     1441 ctgggtaggt tattgatact gaaccttgaa ggtcgcatcc gcattagcag cacccggggt
     1501 tgcagcaccg gttgcaaaat aacgcgcctg gaacggaatg gtgttagtac cgttattcag
     1561 ggttgtttct gaactaaatg tcgcaccgtc cagcgtcagc gcaacacctg ttctgtccag
     1621 aatctgcaca ccaacatttg ttgcgctacc cgcagccgaa ctctgcagag ccagaacgtt
     1681 ggtatgagcc gcatcaatcg ccgtacctaa aaaggcaaca gctgctttag atgcaacatt
     1741 ggtatcgcaa tcattcagct gaatgttaaa accgacagca gaactggttg ctccttcctg
     1801 cgccagcgat gcggtacgaa cctgtcctaa ctgaacggtt tgatcaacag agcctgcatc
     1861 aactgcgcaa gcggcgttaa caacttcccc tttaaagtga acggtcccac cattaaccgt
     1921 cgtggcagcg gccagagccg ctgtggaact gagggacaga gccgacagaa caacgattgc
     1981 cagagtttta attttcatgc tgctttcctt taaaaaaact atttctaaat cgacatgggc
     2041 agtcgttctg tacactttgt tttgtcaacg agtttgactg ccaacactgc acagttttcc
     2101 cccaaaagat gaaacatttg gggccatttt gactcataga ggaaagcatc gcgc
//
LOCUS       DADSZD010000317         2144 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.319, whole genome shotgun sequence.
ACCESSION   DADSZD010000317 DADSZD010000000
VERSION     DADSZD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.319"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            124..1641
                     /gene="lysS"
                     /locus_tag="J1Z28_004461"
     CDS             124..1641
                     /gene="lysS"
                     /locus_tag="J1Z28_004461"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBA7719210.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARN
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
     gene            complement(1760..1933)
                     /gene="ghoT"
                     /locus_tag="J1Z28_004462"
     CDS             complement(1760..1933)
                     /gene="ghoT"
                     /locus_tag="J1Z28_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBA7719211.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(1961..>2144)
                     /gene="ghoS"
                     /locus_tag="J1Z28_004463"
     CDS             complement(1961..>2144)
                     /gene="ghoS"
                     /locus_tag="J1Z28_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBA7719212.1"
                     /translation="IVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDF
                     DLSIMTVDDYFRQFYK"
BASE COUNT          602 a          457 c          503 g          582 t
ORIGIN      
        1 cttgaggtaa acgttagttt cgataagata aactgagtta ttaatagtcg aggcagataa
       61 tacagtgtac cgatctgtct ctttatctac gctaaattga aagctggatt tagaggaacc
      121 aaaatgtctg aacgagaaac acggggagcc aatgaggcta ttgattttaa cgatgaactg
      181 agaaatcgcc gcgaaaaact ggcggcacta cgtcagcaag gtgtggcgtt tcccaatgat
      241 tttcgccgcg accatacctc tgaccagttg cacgaagagt ttgatgcgaa ggataaccag
      301 gaactggaat ccttaaacat tgaagtctcg gttgctggcc gaatgatgac ccgtcgtatt
      361 atggggaaag cctcctttgt aacgttgcag gatgtcggtg gccgtattca actgtacgtt
      421 gcaagaaata gcctgccaga aggtgtttat aacgatcagt ttaaaaaatg ggatctgggt
      481 gacattatcg gtgcccgcgg tacgctgttt aagacgcaaa cgggtgagct ttccattcac
      541 tgtactgagc tgcgcctgct gactaaagca ctacgtcctt taccagataa attccatggt
      601 ctgcaggatc aggaagtccg ttatcgtcaa cgttatctgg acctcatcgc taacgataaa
      661 tcccgtcaaa cgtttgttgt ccgttcaaaa attctggccg ctatccgtca gttcatggtc
      721 gcacgcggct ttatggaagt agaaaccccg atgatgcagg taattccagg tggggcatct
      781 gctcgcccgt ttattaccca tcataacgct ctggatttag atatgtacct gcgtatcgcg
      841 ccggagctgt atctgaaacg tctggttgta ggcggttttg aacgggtatt cgaaatcaac
      901 cgtaacttcc gtaatgaagg tatttctgtt cgccataatc ctgagttcac aatgatggaa
      961 ctctacatgg catatgcgga ttaccatgat ttgattgaac tgacggagtc actgttccgc
     1021 accctggcac aagaggtact gggtaccact aaagtcactt atggcgagca tgtgtttgat
     1081 ttcggcaaac cgtttgaaaa actaaccatg cgcgaagcaa tcaaaaaata tcgtccagaa
     1141 accgatatgg cggatctgga taattttgat gctgctaaag cattagctga atctatcggt
     1201 attacggtag agaaaagctg gggactggga cgtattgtta cggagatctt tgatgaagtg
     1261 gcagaagcac atctgattca gccaaccttt attacggaat atccggcaga agtgtccccg
     1321 ctggcgcgcc gtaatgatgt taacccggaa atcaccgacc gttttgaatt cttcatcggt
     1381 ggtcgtgaaa ttggtaatgg ttttagcgaa ttaaacgacg cagaagatca ggctgaacgt
     1441 ttccaggaac aggttaacgc taaagctgca ggtgacgacg aagccatgtt ctatgacgaa
     1501 gattacgtga ctgcgctgga atatggtctg ccgccaaccg ctggtctggg tattggtatc
     1561 gaccgaatga ttatgctgtt tacaaacagt catactattc gcgacgttat tctcttcccg
     1621 gcgatgcgcc cactgaaata aatttcactt taatgaacga agcagtcagg cgactgcttc
     1681 gttcgtcatt ccgcaaatcc agttttgagg agagacgctt cgtttcaaga taaccagcgg
     1741 gtccactcaa ctacagctcc taaaagagag aaaaaagtaa tgccacaggc agactcattg
     1801 gccaggttat tccgaccaga aatgcactaa gtaaacgaat atgtgttttc tcatgtgaga
     1861 taaaccagat aatgacaaag gatatgttca caccaatcac ataaaaaatt aatattttag
     1921 agaatagtgc cattttttat gttcttctta tccttcctgg ctacttgtaa aactgacgga
     1981 aatagtcatc aactgtcata atgctgaggt caaaatcaat atccggtaag tctaagcatt
     2041 tgagcctgat taaatcttcg atttcacttg cggctaacga tgtcgttatt gcgaaggagt
     2101 tttcattcag ttgtcgtggt atcccatatt catcagccac gata
//
LOCUS       DADSZD010000318         2130 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.320, whole genome shotgun sequence.
ACCESSION   DADSZD010000318 DADSZD010000000
VERSION     DADSZD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.320"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            163..621
                     /gene="tnpA"
                     /locus_tag="J1Z28_004464"
     CDS             163..621
                     /gene="tnpA"
                     /locus_tag="J1Z28_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBA7719213.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGCILRKL
                     CEWKSVRILEAECCADHIHMLVEIPPKMSVSGFMGYLKGKSSLMPYEQFGDLKFKYRN
                     REFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSIPYPGSPFTGRK"
     gene            complement(736..978)
                     /locus_tag="J1Z28_004465"
     CDS             complement(736..978)
                     /locus_tag="J1Z28_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I addiction module toxin, SymE family"
                     /protein_id="HBA7719214.1"
                     /translation="MLSAQRLPNRSDTLVIKISAKWLREAGFEIGTGVTVKISEGCIV
                     LMADGNELQELREQLYQTKQVVKGMRDGMFSVLNES"
     gene            1124..1222
                     /locus_tag="J1Z28_004466"
                     /pseudo
     CDS             1124..1222
                     /locus_tag="J1Z28_004466"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1481..2106
                     /locus_tag="J1Z28_004467"
                     /pseudo
     CDS             1481..2106
                     /locus_tag="J1Z28_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706529.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          555 a          512 c          658 g          405 t
ORIGIN      
        1 aaatcttccc ggctttcttg tgcacagaaa acccccagct aggctggggg ttccggaaag
       61 ctttcagctt tgaaccagtt attaaaaccc cttttgattt gttaaaacac cttgcggtct
      121 ggcaactgca agtgtcaaac aagaaatcaa aagggggtcc caatggggaa cgaaaagagc
      181 ttagcgcaca cccgatggaa ctgtaaatat cacatagtat ttgcgccaaa ataccgaaga
      241 caggtgttct acagagagaa gcgtagagca ataggctgta ttttgagaaa gctgtgtgag
      301 tggaaaagtg tacggattct ggaagctgaa tgctgtgcag atcatatcca tatgcttgtg
      361 gagatcccgc ccaaaatgag cgtatcaggc tttatgggat atctgaaagg gaaaagcagt
      421 ctgatgcctt acgagcagtt tggtgatttg aaattcaaat acaggaacag ggagttctgg
      481 tgcagagggt attacgtcga tacggtgggt aagaacacgg cgaagataca ggattacata
      541 aagcaccagc ttgaagagga taaaatggga gagcagttat cgattcccta tccgggcagc
      601 ccgtttacgg gccgtaagta acgaagttgg atgcaaatgt cagatcgtgt gcgcctgtta
      661 gggcgcggct ggtaagagag ccttataggc gcatttgaaa aacctccggc tatgccggag
      721 gatatttatt attatttaac tctcgttcag cacactaaac atcccgtccc gcatcccctt
      781 aaccacctgt ttagtctgat aaagctgctc gcgcagctcc tgcaactcat taccgtcagc
      841 catcagcaca atacacccct ccgagatctt cacggtaacg cccgtcccga tctcaaatcc
      901 cgcttctctc agccatttgg cgctgatttt gatcaccagt gtatcactgc ggttgggaag
      961 acgctgtgct gacagcacga cgggtagagc cggatggctg ctgtcaggcg taccgttaca
     1021 acgcactgaa cgtggtcaca gagatggtgt tcacccgcgg ccggatgccg gggaaacgga
     1081 cgccccggag attatccgtt tcagttacga cagactgggg cggatgctga gcgaggagac
     1141 ggcgcagggc atcctgaccc atcagtacga tgaactcagc aaccggactg ccaccacctt
     1201 tccggacggc agaacgcagc gtcatctgta ctacggcagc ggccatctcc agcagataaa
     1261 cctggaccgg gaggtcatca gcgagttcac gcgcgaccac ctgcaccgtg aagtgctcag
     1321 aagtcaggga cgactgagca cgcggcagct ttacgacccc ggcgggcgac tgaagcgcag
     1381 ggagacatac agcgggatgc gcggggtggt cccggagacg ttcacggacc ggcagtacag
     1441 ctacagcggc gaggatgaac tgctgaagac gcggcacagc cggcgggggg aaacggacta
     1501 cttctatgac accacggggc gcatcacggc gtgtcgctcg gaggatgagg ggtatctggc
     1561 aagctgggag tacgacgcgg cgggcaacct gctggacagg cgcgcggggg gaagggccac
     1621 ggcagaaaac agcgtggtgc cgttcaaccg gctgatgtct taccgtggtg tgcactaccg
     1681 ttatgacgaa tttggccgtg cggtggaaaa ggaggggcgg agcggaacac agagttaccg
     1741 ttatgatgcg gaacaccgga tggtggaagt gacgacagcg cgggagacat accggtatgt
     1801 gtacgatgcg ctggggaggc ggacggaaaa acagcacatc agccctgacg gtaaaccgta
     1861 caaccggaca aaatttttat gggacggaat gcggctggcg caggagaaca ggccggaggg
     1921 aataagcagc ctgtatatct acagcgaccc gggaagttat gaaccgctgg cgcgggtgga
     1981 taaggcgggg aaagagggac cgaaccggat actgtatttt cacacggatg tgaacggtgc
     2041 gccggaagag atgacggaca gcgacgggaa aatcgtgtgg gagacggggt atcaggtctg
     2101 gggtaacacg atacaggaga aggaccacgg
//
LOCUS       DADSZD010000319         2109 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.321, whole genome shotgun sequence.
ACCESSION   DADSZD010000319 DADSZD010000000
VERSION     DADSZD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.321"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            67..450
                     /locus_tag="J1Z28_004468"
     CDS             67..450
                     /locus_tag="J1Z28_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719215.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..957)
                     /locus_tag="J1Z28_004469"
     CDS             complement(514..957)
                     /locus_tag="J1Z28_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBA7719216.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDRPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVKHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGV"
     gene            1114..2043
                     /gene="metA"
                     /locus_tag="J1Z28_004470"
     CDS             1114..2043
                     /gene="metA"
                     /locus_tag="J1Z28_004470"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBA7719217.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
BASE COUNT          534 a          557 c          469 g          549 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt cccgtaacct taaaacccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccgacccacg ccgcagccgc gcacatcagg
      721 atcgataaac agcgcatcca tatgctgccc acttaacaac ataaacccaa ccggccgatc
      781 ccgctcatta accgcgaccc acaacggcgc ttccggcagg aaggaacgaa ctagctcctc
      841 cagctcggcc cgatactctg ctgataaaaa atcgtgagtg gcatcgacag aacgacacca
      901 aatcgcaacg agttcctccc cttcctcatg ccgtgagcgg cgaatactaa taaccatttt
      961 ctctcctttt cgtcattctt atattctaac gtagcctttt ccttgaaact ttctcacctt
     1021 caacatgcag gctcgacatt ggtaaatttt ctggttatct tcagctatct ggatgtctaa
     1081 acgtataagc gtatgtaatg aggtaatcag gttatgccga ttcgtgtgcc ggacgagcta
     1141 cccgccgtca atttcttgcg tgaagaaaac gtctttgtga tgacaacttc tcgcgcgtct
     1201 ggtcaggaaa ttcgtccgct aaaggtgctt atccttaacc tgatgccgaa gaagatcgaa
     1261 actgaaaatc agtttctgcg cctgctttca aactcacctt tgcaggtcga tattcagctt
     1321 ttgcgcatcg attcccgtga atcgcgcaac acgcccgcag agcatctgaa taacttctac
     1381 tgtaactttg aagatattca ggaacaaaac tttgacggtc tgatagttac cggcgcgccg
     1441 ctgggcctgg tggagtttaa tgatgtcgct tactggccgc agatcaaaca ggtgctggag
     1501 tggtcgaaag atcacgtcac ctcgacgctg tttgtctgct gggctgtaca ggccgcgctc
     1561 aatatcctct atggtattcc taagcaaacc cgcaccgaca aactctctgg cgtttacgag
     1621 catcatattc tccatcctca tgcgcttctg acgcgtggct ttgatgattc attcctggca
     1681 ccgcattcgc gctatgctga ctttccggca gcgttgattc gtgattatac cgatctggaa
     1741 attctggcag agacggaaga aggggatgca tatctgtttg ccagcaaaga taagcgcatt
     1801 gcctttgtga cgggccatcc cgaatatgat gcgcaaacgc tggcgcagga atatttccgc
     1861 gatgtggaag ccgggctaga cccggatgta ccgtataact atttcccgca caatgatccg
     1921 caaaatacac cgcgagcgag ctggcgtagt cacggtaatt tgctgtttac caactggctc
     1981 aactattacg tctaccagat cacgccatat gatctacggc acatgaatcc aacgctggat
     2041 taatcttctg tgatagtcga tcgttaagcg attcagcact ttacctcagg caccttcggg
     2101 tgccttttt
//
LOCUS       DADSZD010000320         2060 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.322, whole genome shotgun sequence.
ACCESSION   DADSZD010000320 DADSZD010000000
VERSION     DADSZD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.322"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>2060)
                     /locus_tag="J1Z28_004471"
     CDS             complement(<1..>2060)
                     /locus_tag="J1Z28_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7719218.1"
                     /translation="DSEQFGSRQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKP
                     AYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGSGGTEPRIT
                     CNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDG
                     APFRYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTS
                     RGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAV
                     NSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGY
                     SVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVN
                     GVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTAR
                     TTETTYRFTQLALGNYRLTVRAANAWGQQGDPASVSFRIAAPATPSRIELTPGYFQIT
                     ATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFY
                     IRSVNTVGKSAFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLD
                     EFSKEWKDANDKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAF
                     IDPA"
BASE COUNT          402 a          671 c          530 g          457 t
ORIGIN      
        1 tttgccgggt caataaacgc gatacggtta gcggcaacca gaaactggct cagcttgcct
       61 tcctccgtgt cctccatgct gaggccaata cccgcgacat aatgtttgcc gtctttggtc
      121 tgctcaattt tgacgcccca catggcattc catttatcgt tagcgtcctt ccactctttc
      181 gaaaactcat ccagtctgct ggcgttatcc tccgtcagat caactttttc cagcagctct
      241 ttaccgagat gggattcggt tatcttgcct ttgaaaaaat ccaggtaacc ttccgcatca
      301 tcgctcgccc gaccgacggc ctccacgaat gccgatttgc caacggtgtt cacactgcgg
      361 atataaaagt aataatcatg gcccggtttg atattgatac tggcggctat ccagtacagc
      421 gccgtaccaa gatagcgggc tgtggtttca acctgcctga tatccgcaat ccgcttttcc
      481 gagaaccaga actcaaactg taccgtcgga tcataaacgg caagatgcgg cgtggcggtt
      541 atctgaaaat agcctggcgt cagctcaatc cgcgacggcg ttgccggtgc ggcgatccgg
      601 aacgataccg atgcaggatc gccctgttgc ccccaggcat ttgccgcccg gactgtcagc
      661 ctgtagtttc ccagcgccag ctgcgtgaag cggtatgtgg tttccgtcgt ccgggccgtg
      721 ctgaccagcc gctcactgcc gtcgtccgct gccacggtaa ggcgaagcat aaagctcaca
      781 cccttcacca ccttcggcgt gtcccagcgc gccagtacct gatactcccc gctgtctgcg
      841 gtgacttcgg cagtcaggtg ctgcaccgct ggcggcgtga caccattcac cgtgccgctc
      901 tggtcgccgt caaagtgcgc cccgttatcc acgatggctt ctttttccgg tacatgctgc
      961 acggcagtga tggcatacgt gccgtcatcg ttctcacgga tactcacgca gcggaacagg
     1021 cgctggcgca gcgtcggcaa cttcagcccc cacacgctgt atccggcaac gccgtcagga
     1081 acacggctca cttttacctt cacaccgtcg gtgacggact ggacctccac gctgaccgga
     1141 ttgccacttc cgtcaaccag gcttatcagc gtggtaccgg aggatggcag cgtgatttca
     1201 cggtcgagcg tcagcgtccg ggtctggctg ttcaccgcca gcacgcgccc gccggtgctg
     1261 ataccggcat agtcatcatc gcagatttca atgacatcgc ccggtacatg gcgaagccct
     1321 tcagcaccca cgctgaagtc cacggtctgc gtttccagca gctccgtttt aatcagccac
     1381 agcccggcgc ggtgtgcctg cccccgactg gtacagccaa aggcatccat cttcgtgaca
     1441 ttacgaccgt aacgggcaat ggcctgcgta tcttcaacaa gctctgtcgc cgtctcccag
     1501 ccgttatccg ggtcaatcca gttcacctca acggcattat ggcggtcctt cagggcgctg
     1561 aagctgtagc ggaacggcgc gccatcatcc ggcatcacca cattactgcg gttataggtc
     1621 cacaccttat ccgacggccg gtcctgcacg aacgtcagcg tctgcccgtt ccataccggc
     1681 atacagcgca tcgccgagca gaaatcactg agcacatccc acgccttgcg ctgtgtggtc
     1741 aggtacgcat tacaggtaat gcgcggctcc gtgccgccag agccgtccgg caccgactgg
     1801 tcgcagtact ggccgatgac atacagcgcc catttatcca catctgccgc accaagacgt
     1861 ttccccatgc cgtagcgcgg gtgggtcagc atatcccaca gacaccaggc catgttgttg
     1921 ctgtatgccg gttttaacgt tccgtcccag ataccgctgt attgccgcgt ctgcgggtta
     1981 tagttcgacg gcacctgcag aatgcgcccg cgaagatgat aattacggct cacctgccgg
     2041 ctgccgaact gctccgaatc
//
LOCUS       DADSZD010000321         2017 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.323, whole genome shotgun sequence.
ACCESSION   DADSZD010000321 DADSZD010000000
VERSION     DADSZD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.323"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..925)
                     /locus_tag="J1Z28_004472"
     CDS             complement(<1..925)
                     /locus_tag="J1Z28_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7719219.1"
                     /translation="MMKIEPSILPSLAWFALIVRAGSFSRAASEMGITRAALSQNLKS
                     LEERLNTKLIYRTTRNMSLTEEGQHLYEVLVSALGQIDDALKDVGDTQLEPTGLLRIN
                     SSRVAARMLVEPHIGEFLTRYPKTKIELIMDDGLSNIIADGCDVGIRLEQGLDEHMTA
                     VPVSPLIKLVTVASPDYLKEHGIPETPQELSNHNCLRLRHKSSGALSAWEFSNVVGGN
                     EEFEIEVSGKYISNDDESMIRMALNGTGIIQHLDFAIAEHINAGKLQPILEDWAVSFP
                     GFYIYVSSRVRMPSKVRAFIDFMVEKRVKIES"
     gene            1082..2002
                     /locus_tag="J1Z28_004473"
     CDS             1082..2002
                     /locus_tag="J1Z28_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBA7719220.1"
                     /translation="MKTVSIKHTYWDIAADIYFPDDFNSEKKYPAIISAHPIGSCKEQ
                     TSGSVYGAALAKAGFIVIAFDASFQGSSGGEPRYLEDPTMRVKDFSIVVDYLTTLPYV
                     DAGRIGVLGICGGGGYAINAAMTERRIKAIGTVTGANYGRLMREGFTAFNPIGALEAM
                     AQQRTDEANGAKLRVDDLLPSSPEAALEAGLTEIDLFEATEYYRSPRGCAPNGVNRSL
                     FSHQTVAVGWDAFHLAEVLLTQPLMVVVGDRVGAFGAYRDGCEIIGRAASKHKELVVV
                     EGYSHYDLYDKPEPVKQALDKLIPFYKTHL"
BASE COUNT          585 a          452 c          432 g          548 t
ORIGIN      
        1 aactttctat ttttactctt ttctccacca taaaatcaat aaaagcacga actttagagg
       61 gcattctcac ccgtgatgaa acatatatat aaaatccagg aaatgaaacg gcccaatcct
      121 caagaatcgg ctgtaacttg cccgcattga tatgttcagc gattgcaaaa tccagatgtt
      181 gaatgatacc cgttccattc agcgccattc gtatcatgct ttcgtcatca ttagaaatat
      241 acttacctga cacctctatt tcgaactctt cattaccccc cacaacattc gaaaactccc
      301 atgctgagag tgcaccgcta cttttatgcc gcaaccgaag acaattatgg ttactcaatt
      361 cctgaggagt ttcaggaata ccgtgttctt ttaaataatc tggcgatgcc acagtaacca
      421 gtttaataag cggagaaaca ggaacggcag tcatatgctc atccagtcct tgctcaagac
      481 gtatgcccac atcacagccg tccgcaataa tgttagataa accgtcatcc ataatgagtt
      541 ctatctttgt tttagggtaa cgagtaagaa actcgccaat atgaggctca acaagcatcc
      601 ttgctgcaac acgggaggaa tttattctca acagtcctgt aggttcaagt tgggtatcac
      661 cgacatcttt caacgcatca tcaatttgcc ccaaagccga caccagcact tcatacagat
      721 gctgtccttc ctcagtaagc gacatattgc gggtcgtacg gtatatcagc ttggtgttaa
      781 gacgttcctc aagagatttt aaattctggg acaatgcggc ccgcgtaata cccatttcag
      841 aggctgctct ggaaaaactg ccggcacgaa caatcagcgc aaaccaggca agagaaggca
      901 aaattgaagg ctctattttc atcattgtaa accttaactt aacactgtga tagatattag
      961 accattgatg ataaccaata caagtcatac tattacgccg ttcgcgatat gaacacgaat
     1021 gccagttgcg gataatagaa atgatattta aatctgttta ggcaaatccc tggagcttga
     1081 aatgaaaact gtttcgataa aacacactta ctgggatata gctgctgata tctatttccc
     1141 tgacgatttt aatagtgaaa aaaagtatcc tgccattatc agtgcccatc caattggaag
     1201 ctgcaaagaa cagacatcgg gtagtgtgta tggtgccgcc ctggcaaaag ctggctttat
     1261 cgtcattgct tttgatgcca gttttcaggg aagcagcggt ggagaaccgc gttatctgga
     1321 agatccaaca atgcgagtaa aagacttcag cattgtcgtt gattacctta ccaccttacc
     1381 ttacgttgat gccgggcgta ttggtgtact gggtatttgt ggtggaggtg gatatgccat
     1441 taatgccgca atgacagaac gtcggattaa agccattggt actgtcacgg gtgcaaacta
     1501 cgggcgtctt atgcgtgagg gatttacagc gtttaatcct atcggagcac tcgaagcaat
     1561 ggcgcaacaa agaacagacg aagctaacgg cgcaaaactt cgcgtagatg atttgctccc
     1621 ttcttctccg gaagcagctc ttgaagcggg attaacagaa atagaccttt ttgaagcaac
     1681 agaatattat cgttcgccac gaggctgtgc gcctaatggg gtaaatcgtt cgctattttc
     1741 acatcagact gttgccgtgg gctgggatgc ttttcatctt gcagaagtcc tgttaactca
     1801 accactaatg gttgtagtgg gcgatagagt gggcgctttt ggtgcctatc gtgacggctg
     1861 cgaaattatt ggtcgtgcag cctcaaaaca taaagaactg gtcgtggttg aaggttattc
     1921 acattacgac ctgtatgata agccagaacc tgtgaaacag gcgctcgata aattaattcc
     1981 tttttacaaa acacatttgt aataactttg cagggag
//
LOCUS       DADSZD010000322         1988 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.324, whole genome shotgun sequence.
ACCESSION   DADSZD010000322 DADSZD010000000
VERSION     DADSZD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.324"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            22..558
                     /locus_tag="J1Z28_004474"
     CDS             22..558
                     /locus_tag="J1Z28_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBA7719221.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVICKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVDAWLDNPFDFTTARENSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
     gene            562..1932
                     /locus_tag="J1Z28_004475"
     CDS             562..1932
                     /locus_tag="J1Z28_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBA7719222.1"
                     /translation="MELITQFINDLGNFIFIPVIFLVLMKILGRPLSECISSAIKVGI
                     GFIALTMTIKLMLEKMQPAVTGLAEATGSSLSAIDVGGAATAVMGFGSSMGAIIIPLC
                     VAVNIAMLVARLTDCVNVDVFNLHQNASMGAIVGVYSGSFLYGALTAALFHVWALIAA
                     DLGAKNNEKFFNLPKGVAISHPVANTYLLFAYPFNWIYDRIPGFRNLNVTAETIQKRF
                     GILGDPTMVGFIIGILLGFCGYGWKSPYHTIIASLQLGMYLAAVMLLLPRMTSIMMEG
                     LVPLSNVARKKLVKRFPDRQITVGMDTALIVGHPSVIAPALLLIPVIVILAVILPGNR
                     VMPLGDLSQFVFFIACMVPVFNGNIIRTWVTSIILFGGGLYIASWMAPATNEVFQKFG
                     TNPDASVMYSSLNPSANPFTGLFAALSHVGIIGYVMAGILLLSIGYLIKQKSRRQIET
                     DLEKAL"
BASE COUNT          508 a          448 c          468 g          564 t
ORIGIN      
        1 cttaacgaca ggcaaaatga aatgaaaacg aagattgcta tcgcaagcga tgatgtaggt
       61 tttgcacgta aagaggagat aaaaaaatac ctcatagaag agaaaaacat ggagatcgtg
      121 tacgatcccg tcatatgtaa ggaacaaggt gtcaataact tcgcgcgtct ggccgacgaa
      181 atggcaggca ttattcaacg cgatgagtgc cgtctgggaa tttacatttg tgggactggc
      241 attggcttta cttgtcagat caacaaacac tggggcataa gagccgttgc ggttactaac
      301 ccctactccg caaagcgggc gcgactcagc aataacgcac aagttattgg actgggttgc
      361 cgggtcaacg atctcgaata cagcaaaatg attgtcgatg catggttgga taacccattc
      421 gacttcacca ccgccagaga aaattctaaa aagaacttac tggaagctga acgtaacgat
      481 aatgcattgc tggttaagcc cgctgatgta cgctggaata tgggtttccg accagatgat
      541 gaaaaatctg agggttaatg tatggaactg atcacgcaat ttataaacga tctgggaaat
      601 tttatattta tcccggtcat ctttctggta ctgatgaaaa tacttggccg tcctctttca
      661 gaatgtatct cctccgccat caaagtcggc attggtttca ttgcgctaac catgaccatc
      721 aaactgatgc tagaaaaaat gcaaccggca gtcaccggat tagcagaagc aacaggttcc
      781 tcgctcagtg ccatcgatgt tggtggcgca gcgactgcag tgatgggatt tggctccagc
      841 atgggcgcta tcattattcc cctctgtgtt gcggtaaata ttgcaatgct ggtcgcccgc
      901 ctgactgact gtgttaacgt tgatgttttc aaccttcatc aaaatgcgtc aatgggggca
      961 attgttggcg tctattctgg tagcttcctg tatggcgcat tgaccgccgc gctattccat
     1021 gtctgggcgc tgattgctgc cgatctcggt gctaaaaata acgaaaaatt ctttaacctg
     1081 ccaaaaggtg ttgcgatctc tcacccggtt gccaatacct acttactttt cgcttatcca
     1141 ttcaactgga tttatgatcg catcccaggc ttccgtaatc tgaatgtgac cgccgaaact
     1201 attcaaaaac ggtttggcat tctgggcgat ccaactatgg ttggttttat tattggtatt
     1261 ttgttgggct tttgtggtta tggctggaaa tccccctacc acaccataat tgccagccta
     1321 cagttaggga tgtatctggc tgcagtcatg cttttgttgc cacgtatgac ctctatcatg
     1381 atggaagggc ttgttccgct ttccaacgtg gcacgcaaaa aactggtcaa acgtttcccg
     1441 gatcgtcaaa tcactgttgg aatggacact gccctgattg tgggtcatcc gtcagttatc
     1501 gccccggcat tattactgat cccggtgatt gtgatcctcg cagtgatctt gcccggcaac
     1561 cgcgttatgc cactgggtga cctctctcag tttgtgtttt tcattgcctg catggtgcct
     1621 gttttcaatg gcaacattat tcgcacctgg gtgacctcga tcattttgtt cggtggtggt
     1681 ctgtatattg catcgtggat ggcaccggct accaacgaag tcttccagaa gtttggtaca
     1741 aacccggatg ccagcgtgat gtactcttcg cttaacccgt ctgcgaatcc atttactggt
     1801 ctgtttgccg ccctgagcca tgttggaatc attggctatg tgatggcagg tatccttttg
     1861 ttatctattg gatacttaat taaacaaaaa tcacgtcgcc agattgaaac ggatttagaa
     1921 aaagcgcttt aaatctttta cagaggaata atcaaatgaa aaaaatctta gtagtttgcg
     1981 gaaacgga
//
LOCUS       DADSZD010000323         1971 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.325, whole genome shotgun sequence.
ACCESSION   DADSZD010000323 DADSZD010000000
VERSION     DADSZD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.325"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..424)
                     /locus_tag="J1Z28_004476"
     CDS             complement(<1..424)
                     /locus_tag="J1Z28_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA7719223.1"
                     /translation="MYEGWYQLRIAGRDVSEQELHSRVCEPLNDGDVIHLVPRTEGAK
                     NGGVLQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMMVGGITQMLTPRAKTPSATTTD
                     NGKRNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDI"
     gene            complement(484..1227)
                     /locus_tag="J1Z28_004477"
     CDS             complement(484..1227)
                     /locus_tag="J1Z28_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7719224.1"
                     /translation="MEQTESAILAHARRCAPAESCGFVVRTPEGEQYQPCVNISAEPE
                     AYFRIAPEDWLRADMQGEIVALVHSHPGGLPWLSEADRRLQVKSAIPWWLVCRGEIHH
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFAREDDWWRNGQNLYLDNLAEN
                     GFYRVSLSGAQAGDILLCCFGSSVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSVSAFTGIYNDLVAASACM"
     gene            complement(1233..1931)
                     /locus_tag="J1Z28_004478"
     CDS             complement(1233..1931)
                     /locus_tag="J1Z28_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBA7719225.1"
                     /translation="MQDIHEESLNEAVKSAQSARVVLWEIDLTAQGGERYFFCGELNE
                     KGEPVTWQGRQYQAYPIEGCGFEMNGKGSSARPSLIVSNLFGLVTGMAEDLQSLVGGT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQLSVLTCEKATFILATPAETDG
                     ALFPGRIMLANTCPWDYRGEECGYNGPAVADEFDKPTTDIRKDKCSKCLRGCEMRGNV
                     ANGGFFLSINKLSQ"
BASE COUNT          443 a          651 c          475 g          402 t
ORIGIN      
        1 tgatgtcctg ggatatccgc cgcgacccca caagcatctc accataaggc accggcatcg
       61 ggttaccctg ggcaatcatg ttgtccagtg aggagaaata cgtgttccgt ttaccattat
      121 ctgttgtcgt ggcagacggt gttttcgccc tgggggtgag catctgcgtg atacccccca
      181 ccatcatggc caccccaaga ttaccgatga cagcaccaat accggtccag ctgaacacat
      241 atcccacaac agccagaaca gcaccggcca ccacctgaag aacaccgccg ttttttgccc
      301 cctccgtccg gggcaccaga tggataacat ccccgtcatt caacggctca catactctgg
      361 aatgaagctc ctgttcagat acatccctgc ccgcaatgcg taactgatac catccctcat
      421 acagtttctg ccggaatccc ggcacctgca gcgacagggc acggatggcc tccgctgccg
      481 tgttcacata caggctgatg cggcgaccaa atcgttgtaa atccccgtga aggcagatac
      541 ggaccagtgg cggtgacgcc agacagaatg cgttcgtcgt tgccattttt cagaatacct
      601 ctcccgttta ctcagttgtt caggtatatg gtgaagcagc tcaccattgc cacagtaaat
      661 ggcagcatga ttgggtaccg atgaaccaaa acagcacagc agaatatctc ccgcctgtgc
      721 accggacagg gatacccggt aaaagccgtt ttccgccaga ttgtccagat acaggttctg
      781 accgttgcgc caccagtcat cctcacgcgc aaaatccggc agcgttatcc ccgccagatg
      841 gtatgcatcc cggaacaggg tgtaacagtc cgtcacaccg tgttcaaaac gccgtccggt
      901 gaggtgcgga acgcagcgga aatgatgaat ttcgccccgg cagaccagcc accagggaat
      961 tgcacttttt acctgcagcc gccggtccgc ctcgctcagc cagggcagac cgccgggatg
     1021 actgtggacc agcgccacaa tctccccctg catatccgcc cgcagccagt cttccggcgc
     1081 aatacgaaaa tacgcctccg gctctgcaga aatattcaca cacggctgat actgctcccc
     1141 ctccggcgtt ctcaccacga agccgcacga ctccgctggc gcacaccgcc gtgcatgcgc
     1201 cagaatggct gattcagtct gttccatgat aatcactgtg aaagtttatt gatggaaagg
     1261 aaaaaaccgc cgtttgccac attcccgcgc atttcacaac cgcgcaggca cttgctgcat
     1321 ttgtctttcc tgatatccgt ggtgggtttg tcgaactcgt cagccactgc cgggccgtta
     1381 tacccacatt cctctccacg gtaatcccac ggacaggtat tggccagcat gatacgaccg
     1441 ggaaacagcg ccccgtccgt ctctgccggt gtcgccagga taaaggtcgc cttttcgcag
     1501 gtcagcactg acaactgctc caccacccag cgatctgtca actcctgctc agggtctgcc
     1561 tccgggtttc ccgcaacaaa attcacggca tcgagaaaac gggcgtacac ccggcggcga
     1621 accaccgtgc ccccgaccag actctgcaaa tcctccgcca tgccggtgac cagtccgaac
     1681 agattcgaga caatcagcga cggtcgcgca ctgctgccct tgccgttcat ctcaaagccg
     1741 cagccctcta tcgggtatgc ctgatactga cgcccctgcc aggtgacagg ctcgcccttt
     1801 tcattgagtt caccgcagaa aaaatagcgt tcgcccccct gcgccgtcag gtcgatttcc
     1861 cacagcacca cgcgggcgga ctgcgctgat tttactgcct cgttcaggct ctcctcatga
     1921 atatcctgca tatgtatccg cccataaaaa aggggcgcag acgcgccccg a
//
LOCUS       DADSZD010000324         1962 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.326, whole genome shotgun sequence.
ACCESSION   DADSZD010000324 DADSZD010000000
VERSION     DADSZD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.326"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(77..1489)
                     /locus_tag="J1Z28_004479"
     CDS             complement(77..1489)
                     /locus_tag="J1Z28_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase I"
                     /protein_id="HBA7719226.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          494 a          453 c          481 g          534 t
ORIGIN      
        1 gcttctggtt atcgattaaa taaaaaaagc gcccatcagg gcgcttcgat atacaaatta
       61 attcacaaaa gcaatattac aggacgtgaa cagatgcggt gttagtagtg ccgctcggta
      121 ccagtgcacc agaaaccata actacgacat cacctttgtg tgccagaccg ctctgcagag
      181 ccagttcttt acccagacgg tagaagtcat cagtagaagt gatctcttta accagctgcg
      241 gcacaacgcc tttgctcagt actaactggt gagccgtttt ttcgttggtg gtcagtgcca
      301 ggatggtggc atccgggaag tatttacgta ctgcgcgagc agatttaccg ccctgagtag
      361 caaccacgat cagtggagca tccagttttt cagcagtttc aacggcacca cggcataccg
      421 cttcggtaat acgcagttta cggttgtcat tgttgaactc gagacggctg ttcatcacgc
      481 ggtcggtacg ttcgcagatg gtcgccatga tagaaaccgc ttccagcggg tatttacctt
      541 ttgcggattc accagacagc atcactgcgt cagtaccgtc gaggatggcg tttgcaacgt
      601 caccggcttc tgcgcgagtc gggcgtgggt ttttgatcat ggaatccagc atctgggtcg
      661 cagtgataac gactttacgt gcacggatac atttttcgat catcatcttc tgggcgaaga
      721 taacttcttc taccgggatt tctacaccca ggtcgccacg cgcaaccatg atgccgtcag
      781 aggcttcgag gatttcgtcg aagttgttga ggccttcctg gttttcgatt ttggagatga
      841 tgtggatgtt ttcgccgccg tgcgctttca ggtgctcacg gatttcgata acgtcagaac
      901 gcttacgaat aaaggaagca gcaacaaagt ctacgccttg ttcgcaacca aagatcaggt
      961 cctgtttgtc tttttcagcc agtgctggca gggcaatgga aacgccaggc aggttcacac
     1021 ctttgttttc gcccaggtcg ccgttgttca gcactttaca gataactttg ttaccttcaa
     1081 tggcggtaac ttccataccg atcagaccgt cgtcaaccag tacggtgttg ccaacagaca
     1141 ggtcagtagt gaaaccttca tacgttaccg caaccatttc gctgttgccg ataacagatt
     1201 tatcagtagt gaaagtaaag gtctgacccg ctttcagaga aacgtcgtta ccgccttcca
     1261 gtttcatggt gcggatttct ggacctttgg tatcaagcag gatagcggcg gttttaccag
     1321 ttttgctcat cacgttgcgc agattctgaa tgcgctgacc gtgttctgca tagtcaccat
     1381 gagagaagtt cagacgcata acgttcatgc cagcgtccag cattttagct aacatctctt
     1441 cagattcggt tttcggtccg atggtgcaaa caattttggt ctttttcatg acagtcttag
     1501 tctttaagtt gagaaggatg ggagaaactt gctttctggg cgcactgccg gaaagcgaat
     1561 ccggtattac gaaagtggtt gtgcctcgct ctaaggatag gtgacatcga aaaagcgtgc
     1621 agaggaatgt gtgcttgtgt ctcagcccaa cgtgagtgcc tgtttttgag tcgttgagtt
     1681 ctacagtcca aagtgctgaa accattcaag agtcaattgg cgcgcattat acgctgaaat
     1741 taatccaaaa ggaaaacaaa aacagcgttt caaaaaatat acaacttgta agacaataaa
     1801 ttgttatcaa agattcagcg ttccagggaa aaggttacgc aaaaacttcg ttgtttgcgc
     1861 aacgtctggt gccggggttg tcagtgtgag gataagtgaa agttcatccg gtgtataaat
     1921 acatcatcgc tcaattgcta cagttaaaat aatttatcac gt
//
LOCUS       DADSZD010000325         1925 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.327, whole genome shotgun sequence.
ACCESSION   DADSZD010000325 DADSZD010000000
VERSION     DADSZD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.327"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            39..650
                     /locus_tag="J1Z28_004480"
     CDS             39..650
                     /locus_tag="J1Z28_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HBA7719227.1"
                     /translation="MQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAYGKFMSTIF
                     MRWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIYECDHFVYP
                     QYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRITKVNNETVS
                     YFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(616..741)
                     /locus_tag="J1Z28_004481"
     CDS             complement(616..741)
                     /locus_tag="J1Z28_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719228.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            761..1918
                     /locus_tag="J1Z28_004482"
                     /pseudo
     CDS             761..1918
                     /locus_tag="J1Z28_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
BASE COUNT          569 a          424 c          414 g          518 t
ORIGIN      
        1 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctcg
       61 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
      121 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcgatggg
      181 ttaaaaacga tgtgggtgaa attttcatcc gtcagtttga aagctttgtc agccgttttt
      241 tggggaatgg gcataccagt tgtattttcc aggagtcctg caaggataat ctggttgttg
      301 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
      361 acattaataa atcggaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
      421 ggatttcagc caaatgtcag caatgtgtat ataaacctat ctgcaatggt ggttgcccta
      481 agcatcgtat tactaaagta aacaatgaga ctgtttccta tttttgcgaa ggttataaaa
      541 tccttttttc aaccatggta ccttatatga acgccatggt ggagttagct aagaacagag
      601 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctccc
      661 ccttagctcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
      721 tgtcgggtgt ttcgtcttca tcatcttcct ttgcgaaaat attgccgtat ctaataaagg
      781 cgatttaagt ttccctgcgc agcaaaaatc acttatcgcg ctggctaata aagcagtaag
      841 cggaagcggc gttggtgaac tctcctcttc cagcctgaaa cgctggagtg cggaaaactc
      901 ggtaaccatg agcagtaaag tcagtggcat gaatacgttg ctctccgtta gcgtgcggac
      961 taataaccct gaacctggtt tccagttgat taaccagcga atcacccaca gcacgattaa
     1021 cgacaatatt tgggcatcgc tacaaaatgc tcaaattcag gcgttgaaaa cgctcgacca
     1081 gcgtccagcg gagaaattcg cccagcagat gtatgagacg cgctatgccg atgaccgcac
     1141 gaaattaccg caagaaaatc agattgcaca gtttactgcc gcagatgcgc tggctgccga
     1201 tcgccagttg ttttcatctc cagcggatat cacgtttgtc attgtcggta atgtcgcaga
     1261 agacaaactc gtggcgttaa ttacgcgtta cttaggatct atcaaacact ctgattcgcc
     1321 attagccgca ggtaaaccat taactcgcgc gacggacaac gcatcggtta ctgtaaaaga
     1381 acaaaatgaa ccagtggcac aggtttcaca gtggaagcgt tataattccc ggacacctgt
     1441 taatctggcg acgcgtatgg cgctcgatgc ttttaacgtc gcactggcaa aagatctacg
     1501 cattaatatt cgtgaacagg catctggggc atacagcgtt tcttctcgcc tctcggttga
     1561 tcctcaggcc aaagatatca gtcatttgct ggcttttact tgtcaaccag aacgacatga
     1621 tgaactgtta acgttagcga atgaagtgat ggttaagcgt ctggccaaag ggatcagtga
     1681 gcaagaactg aacgaatacc agcaaaacgt tcagcgcagc ctcgatatcc aacagcgtag
     1741 cgttcaacaa ttagcgaaca ccattgtaaa tagtcttatt caatatgacg atcctgcagc
     1801 atggattgag caggagcaat tgttgaaaca aatgacggta gagaatgtta acactgctgt
     1861 taaacaatat ctttctcatc cggtgaatac gtataccgga gtattattgc caaaataatc
     1921 acagc
//
LOCUS       DADSZD010000326         1912 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.328, whole genome shotgun sequence.
ACCESSION   DADSZD010000326 DADSZD010000000
VERSION     DADSZD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.328"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            66..1088
                     /locus_tag="J1Z28_004483"
     CDS             66..1088
                     /locus_tag="J1Z28_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBA7719229.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1088..1867
                     /locus_tag="J1Z28_004484"
     CDS             1088..1867
                     /locus_tag="J1Z28_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBA7719230.1"
                     /translation="MMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          500 a          494 c          492 g          426 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agtttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactgggga tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttctc ggatacagcc gaatgctgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt
      781 acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg gggatcactg
      841 tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc gctaaccaac
      901 gaaagcatga aacaatccag gcacgtccct gcgatcgatg gctcgaagag cagcagtcca
      961 tgctggcact gcctccggag aaaaaagagt atgacgtgca tcttgatgaa aatctggtga
     1021 acttcgacaa acaccccctg catcatccac tctccatcta cgactcattc tgcagaggag
     1081 tggcgtgatg atggaactgc aacatcaacg actgatggcg ctcgccgggc agttgcaact
     1141 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
     1201 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
     1261 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
     1321 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
     1381 catagaacgt aatgaaaata tcgtgttact ggggccatca ggtgtgggga aaacccatct
     1441 ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc
     1501 agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct
     1561 tcagcgtgga gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt
     1621 cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc
     1681 aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc
     1741 agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa
     1801 aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc
     1861 tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga tc
//
LOCUS       DADSZD010000327         1894 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.329, whole genome shotgun sequence.
ACCESSION   DADSZD010000327 DADSZD010000000
VERSION     DADSZD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.329"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            581..960
                     /locus_tag="J1Z28_004485"
                     /pseudo
     CDS             581..960
                     /locus_tag="J1Z28_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232055.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
     gene            complement(1156..1458)
                     /locus_tag="J1Z28_004486"
     CDS             complement(1156..1458)
                     /locus_tag="J1Z28_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBA7719231.1"
                     /translation="MVLLTAGHLIPDRYVLVDAHQQPVDFTQGEYGLLLALAQNSRRV
                     LICEQLLDLIHGETTEVFDRTIYVLIMRLCRKIEINPHWCFASPIQRLASFWSNLS"
BASE COUNT          570 a          428 c          426 g          470 t
ORIGIN      
        1 gatcgacaaa atcgatcata catgatgaag attttttatc gaatccataa aaatagtgac
       61 agctaaccgg cgttgcagga acagtcagaa atgggcgttt gggaaagagc catagcacta
      121 cgtcgtcgct gacatagagg aactgtgctt tgttgataag atcctttata cggcaaccaa
      181 tccattggac aaaagatgaa ctacgtaatc accgggttct cactgacgaa atacagaagt
      241 taatgacaca actgtgccat gtaccttgta caacagcggt ggaaagctct cagaacaatg
      301 gaattgcaga aaggtgttaa aacgatgaaa gccttcatac ccaaatcgaa tgtaagaacg
      361 gcagtaaaga ctgaattgcg taaccttgca gtagctcgag tattacactg catagtgtgc
      421 agggttatct cccatcgaga aaatatcggc gccagcgaat aacgtcacct tagatgtagc
      481 agttgccaaa tagtgactca agggcgggct taccgcatac actgacactt agcggatcga
      541 cagaatatta ttagcagatc atcactgaac gctacgtaat tatcgtaata aaggcttttt
      601 ctggctacca ggaagacctg acatggctct gctctggaac caggccgcag gaagcatcaa
      661 tctggagttt atcagctact ggaattccgg tgtattggca gcccctgata atcacctgcc
      721 ccacgaagag cgctctgctt tgcagaaatt ctggggcggt ttggagacag gagatgccat
      781 ttaacgatta taggacgttc tgatgaagtc catgatttta cctccgcctt aattaactgt
      841 tttctttctg aagaaaaaat tgtctggtgg caatcaggtg gcattttccc ggatccctgg
      901 cccgctaata tatcccggct gaacagacga ttaacgcgat attgcaaata tggcatctga
      961 atcgatcatg atttggcggc atccatctcc gttcacagtt tggcatgctt ccgatcattg
     1021 attggcatcc agccaatcac tccgggaatt aactcttttc accgcagtct ccattgccgt
     1081 gtttaaacca atggagacgt caatgtccaa aaagagaata tccaggagca ctatggatac
     1141 ctgttttaag atccttcagc tcaagttcga ccagaagctg gctaaccgtt gtatcggact
     1201 tgcaaaacac caatggggat tgatctctat tttgcgacac agacgcatta tcaatacata
     1261 gatggtgcga tcaaatacct cagtggtctc accgtggatc aaatccagca attgctcaca
     1321 gattaagact cgtcgggagt tttgagccaa cgccagcagt aacccatatt caccttgagt
     1381 gaaatctaca ggctgttgat gagcatcaac cagcacgtaa cggtccggga tcaagtgtcc
     1441 agccgttaaa agaaccactc tactaccctg ctcgacctaa gcctcggcgt tcagccgcct
     1501 gaacgggtat ggcaagggtg aaaagaaaca gcatccccac agtaccgacc agacgacagg
     1561 atgatgctgg aacagaaagc attcgcacct cccttagaat tagacagtgc gtacaggata
     1621 cgtaagacag ggtgacgggg cggcgataaa ctctatttac aaagctgaaa attttctgac
     1681 gatgaaaaac tattcaacaa ggttatctga ggcgttaaaa taaccagctc gattaacgac
     1741 taacttgagg tgaatatgaa tttaaaaaat ataattttaa gtactgtttt atcaatcgct
     1801 agttgtcatg ccctggctgt aggtaattct caaatagcgc tatctaacct tcatgtggaa
     1861 aacaccccaa gtgggacgaa ggcaattgtg ggtt
//
LOCUS       DADSZD010000328         1893 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.330, whole genome shotgun sequence.
ACCESSION   DADSZD010000328 DADSZD010000000
VERSION     DADSZD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.330"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(171..923)
                     /locus_tag="J1Z28_004487"
     CDS             complement(171..923)
                     /locus_tag="J1Z28_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBA7719232.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMAMDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEYCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHSTPVYKVCGRCNGNRFSRLSTTLARCHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEVQLRKVTR"
     gene            complement(937..>1893)
                     /locus_tag="J1Z28_004488"
     CDS             complement(937..>1893)
                     /locus_tag="J1Z28_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBA7719233.1"
                     /translation="RVLVEPEPKSMRNLPSGVVPAVRQPLVEDKTLLPFFSNARVIRA
                     AGGAGALSDWLLRNIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLRDQTSESL
                     EQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVLRRSLGLP
                     AEKNCSVYRESDIVPGEQTATSILTQRTKNLAPLPHAHQQQNPSQEKTVVSITVDPES
                     PESFMKLPKRHRWVKEKYTRWVKTQPCACCGMPADDPHHLIGHGQGGMGTKAHDLFVL
                     PLCGKHHDELHANTVAFEEKYGSQLELIFRFIDRALAIGVLA"
BASE COUNT          457 a          522 c          414 g          500 t
ORIGIN      
        1 aaccataatg gttaccgttc tgcacaaagt ttatgtccac aaaaaaaaca ctctgcgcgg
       61 cttattgata ttttccacag gtatgcatat aaggctcatc attggaaaac tctttgcacg
      121 tttccttaga aaatacaatc gttatgtcgc catcgttcgg tgaaaatcat ttatctcgtc
      181 acttttctta attgcacctc agcatatgct tcttcctgcc agcactttgt aaccagttta
      241 tcaatgacat ctgcatatcc tttgtaccac tgataatccg tcaggtctgg taccagcttc
      301 tggacatgac accgcgccag tgtcgttgat aaacgactaa accggtttcc attgcaacgc
      361 ccacaaacct tataaacagg cgtgctatga agccgggttc ttttttcatc caggacaata
      421 cctttcccct tacaccctct gcacgctgtg ctgacttctc ccttaccatg gcaatgctga
      481 catagttcct tcacccactc ttccttgata acagattccc cacttctgga gtgtttcacc
      541 acttcgcgca atacattatg aaatccagta ccagcacaat actcacagcg agccttactt
      601 gccgcagacc tggaataatc agcaaaggca aaattcacaa ggtaagggat gatctgtaac
      661 cggatttctt cactcaattt gttcaatgtc ggattatcca ttgccatcgc gtaattgagc
      721 agaccttcaa tcgcaaactg aggatcctga acaccaactt ttgccaggaa taaggcaaaa
      781 ccaagcggag ctttcgactg caccatcccc tgcgcagcca tcacatctgt tattgttaaa
      841 ccacccgagc ctgtcgccgg tgcgtcatca ctcaattttg gagattttgg ggagtaatat
      901 tttggtaaag cttcaaggtt catgctcgtt ctccacttac gccagtacgc ctattgccag
      961 cgcacgatcg ataaaacgaa atatcagctc cagctgggag ccatacttct cttcaaatgc
     1021 cacggtattc gcatgcagct cgtcgtgatg ctttccgcac aaaggcaaca caaagagatc
     1081 atgtgctttg gttcccattc cgccctgccc gtgaccaatc agatgatgcg gatcgtcggc
     1141 tggcataccg cagcaagcac acggctgtgt cttaacccag cgtgtgtatt tctccttaac
     1201 ccaacggtga cgtttaggca gcttcatgaa agattccgga gactctggat caacggtgat
     1261 gcttaccacc gtcttttcct gtgatgggtt ttgttgctgg tgggcgtgag gcaacggtgc
     1321 aagatttttt gtgcgctgtg tcaatatgct ggtggcggtc tgctctcccg gtacgatgtc
     1381 gctttcgcgg tacaccgagc agtttttttc cgctggtaat cccagcgagc gacgtaatac
     1441 cgcttccggt agcgcgtccg ccacctgatt gcggaccgcc caccaggata attcagccag
     1501 agataattca cgctcctgcg taccgcttat tgcgtgaccg atgacgtcaa tcatccatgc
     1561 tgacaggttt tgatgagcaa gttgctcgag tgattcggat gtctggtcac gcagctggtt
     1621 gtcgcagtgc cagcacaaca ccattgcgcc ggtaccataa cggtgaataa cggtttcgct
     1681 gtgatgataa tcgccgtgtg gccactggca ggatttaata ttgcgcaaca gccagtcaga
     1741 caatgcacca gcaccaccag cagcacgaat tacccgtgcg ttactgaaaa acggcagcaa
     1801 tgttttgtct tccaccagcg gctggcgaac ggcaggaacg accccggacg gcagattacg
     1861 catgcttttc ggttccggct ccaccagtac ccg
//
LOCUS       DADSZD010000329         1873 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.331, whole genome shotgun sequence.
ACCESSION   DADSZD010000329 DADSZD010000000
VERSION     DADSZD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.331"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            208..711
                     /locus_tag="J1Z28_004489"
     CDS             208..711
                     /locus_tag="J1Z28_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBA7719234.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(789..1040)
                     /locus_tag="J1Z28_004490"
     CDS             complement(789..1040)
                     /locus_tag="J1Z28_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBA7719235.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1155..1241)
                     /gene="azuC"
                     /locus_tag="J1Z28_004491"
     CDS             complement(1155..1241)
                     /gene="azuC"
                     /locus_tag="J1Z28_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBA7719236.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1505..1828
                     /locus_tag="J1Z28_004492"
     CDS             1505..1828
                     /locus_tag="J1Z28_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719237.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTDRYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          492 a          439 c          406 g          536 t
ORIGIN      
        1 tcatcaatga aatgtaaaaa tatataaact tgatgattta agcattttct tatacccgtt
       61 cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac atttcggacc
      121 ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc ttgcacctct
      181 ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct caaactcaac
      241 tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag taactggtgt
      301 tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca gagtaatgtg
      361 acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc catcgttaaa
      421 gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca aaaaacgatg
      481 gaagaatacg agcaacgttc aagcacgctg gcgcagttag ccgatgaagc gaaagaacta
      541 aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca gcatgatggc
      601 ctgttactgc aaaccattct tgatgaagtg cgcagtgcga aacttgctgg tatgtgtcct
      661 gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc agctacattg atcatcatcg
      721 gcgctaatgc attgcgccga tgaagttttt gagaaaccgc tgcctcattt ttttgaagca
      781 gcggtttttt aatgggattc gccctgtggg gtaaacttga gttcaatcaa cgcgatggct
      841 ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttagt aaagtcgata
      901 ttcttcagct ggctggacat tttttcgcga acttcaacgg gcgcgatcac atcgagaaca
      961 tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag ttcctgatcg
     1021 gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc
     1081 ggcttcacat cttcagcgat agtcacatcc accgtcatca gaacacaaaa aaacctgccg
     1141 gagcaggttt tttgttatcg gaacatattg cctggcggta cgtctttgaa cgtcttgcaa
     1201 tagttattga acatactttt caggatcttg cgcagtttca tcgcggtact ccgaccattt
     1261 gttatacagg tgttattgtc tttgcgttta taatatgaca accatcacaa aaatcaatct
     1321 ttatgtgatg taaatcacat aaataccctt ttaatgttat aaaaatgata atcaaaaagc
     1381 agccccccca atttctgaca catacagatg gcaagaaata gcgcctgcca ggcgtctttt
     1441 ccggccattg tcgcagcgct gtaacgcgta aaatactgct ttcttttact cttctggctg
     1501 gaccatgaga cttctgattc tgactctttc cttaataacg ctgacaggat gtacggttac
     1561 tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgttcggt tggtttatga
     1621 tcaggctttt ttgcagcatg cccgtaccga tcgttatgtc agtcgcggga tcgccgatcg
     1681 cgcatgccag caggcaggct atacccacgc tatccccttt ggtcagccag taggcaactg
     1741 tagcctcttt gcgggttcct tatgtttgaa tacggaattc actttgtcat atcagtgcca
     1801 tcattccgcc ttccctgtct ttctctaaac gttttaatca gcgaggggga tcttcgctga
     1861 ttaaataaat agc
//
LOCUS       DADSZD010000330         1867 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.332, whole genome shotgun sequence.
ACCESSION   DADSZD010000330 DADSZD010000000
VERSION     DADSZD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.332"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            121..1254
                     /gene="ompN"
                     /locus_tag="J1Z28_004493"
     CDS             121..1254
                     /gene="ompN"
                     /locus_tag="J1Z28_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBA7719238.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1395..1829
                     /gene="uspF"
                     /locus_tag="J1Z28_004494"
     CDS             1395..1829
                     /gene="uspF"
                     /locus_tag="J1Z28_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBA7719239.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          509 a          428 c          428 g          502 t
ORIGIN      
        1 aaggttttac ttaaggcgta acaaatgata tttatcggct aactgaactt ctcctttagg
       61 atgttttcac tccccaaaat ggggatgaaa ggcaaataaa ataactaaag gatttattca
      121 atgaaaagca aagtactggc actcttaatt cctgccctgc tcgctgcagg tgctgcacat
      181 gcagccgaag tttataataa agacggcaac aaattagatc tgtatggcaa agttgatggc
      241 ctgcattatt tttctgataa ttcagcgaaa gatggcgacc agagctatgc tcgtctgggt
      301 tttaaaggcg aaacccaaat taacgatcaa ctcactggct acggtcaatg ggaatacaat
      361 attcaggcaa acaacaccga atcttcaaca aaccagtcat ggacccgtct ggcatttgcc
      421 ggactgaaat ttgcagatta cggttctttc gattacggac gtaattatgg cgtaatgtac
      481 gacatcgaag gctggactga tatgctacct gaatttggcg gtgactctta taccaatgca
      541 gacaacttta tgactggtcg tgccaatggc gtcgcgactt atcgtaatac tgatttcttc
      601 ggtctggtaa atggtctgaa cttcgcggtg cagtatcaag gtaacaacga aggtgccagt
      661 aatggtcagg aaggcaccaa caacggacgt gatgttcgcc atgaaaacgg tgacggctgg
      721 ggtctttcca caacatatga tttaggcatg ggctttagcg ctggtgcggc atacacctct
      781 tctgaccgca ccaatgacca ggttaaccat actgcggcgg gtggtgataa agcagacgcg
      841 tggactgctg ggctaaaata cgatgctaac aatatttacc tggcaaccat gtattcagaa
      901 acgcgtaata tgaccccgtt tggcgacagc gattatgctg ttgcaaacaa aacccagaac
      961 tttgaagtca ctgcacagta ccagtttgat tttggtctgc gtccggcagt ctctttcctg
     1021 atgtctaaag gccgtgacct gcacgctgcc ggtggtgcag acaacccagc aggtgttgat
     1081 gataaagatc tggttaaata cgccgatgtt ggcgcgactt actatttcaa taaaaacatg
     1141 tccacctatg ttgactataa aatcaacctg ttggatgaag atgacagttt ctacgctgcc
     1201 aacggcatct ctaccgatga tattgtcgct ttaggtctgg tttatcagtt ctaaatcccc
     1261 ctgcccgctg ttttggcggg ctttttctgc ttattcttcc tcctttgatc taaattaaaa
     1321 atgtgaactc cgtcattaca caaaaagtgt catctggcgt tacactttat gcggatacta
     1381 aaacaggagg ttttatgaac agaacgattc ttgtccctat cgatatttcc gattcagaat
     1441 taactcaacg cgtgattagc cacgttgagg cagaggcaaa gattgatgat gcagaggttc
     1501 atttcctgac ggtaatacct tcgctgccct actatgcctc tctgggttta gcgtattccg
     1561 cagaattacc ggcaatggat gacctgaaag cggaagccaa atcgcaactg gaagagatca
     1621 ttaaaaaatt taaactgcca accgacagag tgcatgtcca tgttgaggaa ggctcgccca
     1681 aagaccgcat tctggaattg gcgaagaaga tccccgctca tatgatcatc attgcttccc
     1741 atcgaccgga tatcaccact tatctgctcg gttctaacgc cgcagctgta gtgcgtcacg
     1801 cagagtgctc cgtgctggtt gtgcgctgac actaacgccc gcacattgct gcgggctttt
     1861 tgattca
//
LOCUS       DADSZD010000331         1858 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.333, whole genome shotgun sequence.
ACCESSION   DADSZD010000331 DADSZD010000000
VERSION     DADSZD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.333"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            113..367
                     /gene="bssS"
                     /locus_tag="J1Z28_004495"
     CDS             113..367
                     /gene="bssS"
                     /locus_tag="J1Z28_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBA7719240.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            482..1600
                     /gene="solA"
                     /locus_tag="J1Z28_004496"
     CDS             482..1600
                     /gene="solA"
                     /locus_tag="J1Z28_004496"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBA7719241.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGESEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1648..1761
                     /locus_tag="J1Z28_004497"
     CDS             1648..1761
                     /locus_tag="J1Z28_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2770 domain-containing protein"
                     /protein_id="HBA7719242.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          481 a          448 c          442 g          487 t
ORIGIN      
        1 cctttaaaaa ttcggtgaat acccttactt attggtatac tgaagttgct tcagataaaa
       61 acgcattgaa cctcgaataa cgttgtctag taacacgaat tagggggcca tgatggaaaa
      121 aaataatgaa gtcattcaga ctcatccgct cgtagggtgg gacatcagca ccgttgatag
      181 ctatgatgcg ctgatgttgc gtttgcacta ccagacccca aataagtccg agcaggaagg
      241 gactgaagtt ggtcagacgc tctggttaac cactgatgtt gccagacaat ttatttcgat
      301 attagaagca ggaatcgcca aaattgaatc cggtgatttc caggtaaacg agtatcggcg
      361 tcattaacat gacatgacca tccacccaaa aggcacctca ccggtgcctt taccatttct
      421 gggttgtata tctgcattca gttaattact ctgcttaaac catgagagca gagagaaaaa
      481 aatgaaatac gatctcatca ttattggcag cggttccgta ggcgctgccg ccgggtatta
      541 tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc catatgccac cgcatcaaca
      601 cggcagccac cacggcgata cgcgattaat tcgccatgct tatggtgaaa gcgaaaagta
      661 tgtcccgctg gtcctccgcg cgcaaatgct gtgggatgaa ctttcccgcc acaacgaaga
      721 tgatcccatt tttgtacgct ctggtgtcat taacctcggt ccggctgact ccgcatttct
      781 cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac gttgaaaagc tcgacgcgca
      841 agggattatg gcccgctggc cagaaatacg cgtcccggac aactacatcg gtttatttga
      901 aactgattcc ggttttttgc gcagcgaact ggcgattaaa acctggatcc aactggcgaa
      961 ggaagcgggc tgtgcgcagc tgttcaactg tccggtcacc gcaattcgtc atgacgatga
     1021 tggcgtaact attgaaacgg ctgacggtga gtatcaggcg aaaaaagcga ttgtctgcgc
     1081 gggaacatgg gtaaaagacc tactcccgga gctgcctgtc cagcccgtac gcaaagtatt
     1141 tgcctggtat caggccgatg gccgctatag cgtgaagaat aaattcccgg catttaccgg
     1201 tgagctgccc aatggcgatc aatattatgg ttttccggca gaaaacgacg cgttgaagat
     1261 tggcaaacat aacggaggcc aggttatcca ttcagcggat gaacgtgttc cgtttgcgga
     1321 agtggtcagc gatggttcgg aagccttccc gttcttgcgc aatgtgttgc cgggtatcgg
     1381 ttgctgcctg tacggcgctg cctgcaccta tgataattcg cctgacgaag attttattat
     1441 cgatacccta cccggccacg ataatacact gctcattacc ggcctgagtg ggcacggttt
     1501 taaatttgcg tcagttttag gggaaatagc tgccgatttt gcgcaagaca aaaaaagcga
     1561 ttttgatttg acgccattca ggctttcccg cttccaataa tcatcattac ggcctccaga
     1621 atgaggccgt ttttacagga gtatttaatg cgtcgactgt tgcactatct catcaataat
     1681 attcgcgagc atctgatgct ttatcttttt ctctggggat tgttggcaat tatggatctg
     1741 atctatgtgt tttattttta gttttccgca ctgacataaa ctgacaaata tttaatctcc
     1801 ctttcattta gcgtgaaaaa ttgaatgaga ttcttcctgc ctctacagca ctcactag
//
LOCUS       DADSZD010000332         1831 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.334, whole genome shotgun sequence.
ACCESSION   DADSZD010000332 DADSZD010000000
VERSION     DADSZD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.334"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            134..925
                     /gene="kdgR"
                     /locus_tag="J1Z28_004498"
     CDS             134..925
                     /gene="kdgR"
                     /locus_tag="J1Z28_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBA7719243.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(1069..1308)
                     /locus_tag="J1Z28_004499"
     CDS             complement(1069..1308)
                     /locus_tag="J1Z28_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719244.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            1467..1610
                     /gene="mgrB"
                     /locus_tag="J1Z28_004500"
     CDS             1467..1610
                     /gene="mgrB"
                     /locus_tag="J1Z28_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBA7719245.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            1685..>1831
                     /locus_tag="J1Z28_004501"
     CDS             1685..>1831
                     /locus_tag="J1Z28_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719246.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEAL"
BASE COUNT          486 a          380 c          451 g          514 t
ORIGIN      
        1 ctagcactga tattcacggt ggatgtcgca gaattgtaac taaccggcaa aagatggatt
       61 gcgaccaccc tggtgcgaaa ttataataaa aaacagttct gatttttata aaacactcgc
      121 aatgaggtga taaatggcta acgcagatct ggataaacag cctgattctg tatcttccgt
      181 gctaaaagtt tttggcattt tgcaggcgct gggtgaagag cgcgaaatag ggataaccga
      241 gttgtcgcag cgcgtcatga tgtcaaaaag caccgtttat cgctttttac agaccatgaa
      301 aaccttaggt tatgtggcgc aggaagggga gtcggagaaa tattcgctga ccctgaaatt
      361 gtttgaactg ggcgctcgcg cgttgcaaaa cgtcgattta attcgtagcg cagatatcca
      421 gatgcgtgag atctcccgcc tgaccaaaga aactatccac ctcggcgcac tggacgaaga
      481 cagtattgtt tacatccaca aaattgactc aatgtacaat ttgcgcatgt attcacggat
      541 tgggcgtcgt aatccgctgt acagcaccgc gattggtaag gtactgctgg catggcgcga
      601 tcgcgatgaa gtgatgcaaa ttcttgaggg cgtggagtat aaacgcagta ccgagcggac
      661 catcaccagt acagaagcgt tattacccgt tctggaccag gtgcgcgagc aggggtatgg
      721 cgaagataat gaagagcagg aagaaggact gcgttgcatt gcggttccgg tatttgatcg
      781 ctttggcgtg gtcattgccg gtctgagcat ctccttcccg acgttgcgtt tctctgaaga
      841 gcgtttacag gaatatgtcg caatgttgca taccgcggcg cgcaaaattt ctgcccaaat
      901 gggttatcac gactatccgt tctgatgaga gtaagaacct gtctgaatat caaacagaca
      961 ggttctttat ttagcatgag aaaaataaag ttgaaggtgg cgttatatta aacgcacttg
     1021 cgataagaat attttactca tgagtgagaa tctggttatt tattaccctt aaccattatc
     1081 gaccacaata ttgcttttac gtaacaacgg acaatctgtt atccccagaa aaccactttt
     1141 agtgtgcagg tattgtaccg tgctggtgcc tctggcggtc agataggtac attgtaaacc
     1201 taatcccgcg gcattctctt tgcttccaat caaaacgcca tatccgctga gtaataatcc
     1261 tatccatacc agcgctatca gcataactgt gcgaatgatg aatcgcatta caacctcttc
     1321 tctttttatg ttcgcttaat cgtagcggca atatgcgctg aagcaagcga ctcattccga
     1381 aaaagcacga atatcgacat agttaggcgc tgtttaacta acgcatgcta gtttaatgac
     1441 ataaggtagg tgaaacggag attggagtga aaaagtttcg atgggtcgct ctggttgtcg
     1501 tggtgttggc ttgcttgctg ctttgggcgc aggtattcaa catgatgtgc gatcaggatg
     1561 tacaattttt cagcggaatt tgtgccatta accagtttat cccgtggtga tatcattttt
     1621 tcagcactag attttcttcc tctccagcag tggtagaata gcgcctctac tcagaggtgg
     1681 tgaaatgaac gaagttgtaa attcaggcgt gatgaacatt gcgtctttgg ttgtatcggt
     1741 ggtggttctt cttatcgggc tcatcttgtg gttttttatc aatcgtgcca gttcacggac
     1801 taacgaacag attgaactgc ttgaggcgtt g
//
LOCUS       DADSZD010000333         1811 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.335, whole genome shotgun sequence.
ACCESSION   DADSZD010000333 DADSZD010000000
VERSION     DADSZD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.335"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..1464
                     /locus_tag="J1Z28_004502"
     CDS             <1..1464
                     /locus_tag="J1Z28_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBA7719247.1"
                     /translation="DDMAKLIPFLAIPIIKTILDFIVSLGLFAFVNLHVLFAIAIPLG
                     LLKDKEDKAYGAFSGLIGFMAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNT
                     SVLGGIVAGLLVASMYKKIVNLRIPESLGFYSGPRLVPIITLSVMSGFGLIIPFIWPP
                     FFNLFMLIGHWISTSGPVGYFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEE
                     YYGTLNMFMAYVKENAVIPLDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKA
                     LLISGVLTVIIGGVSEPIEFLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLI
                     QFISFGVLRGTRTGWPIAVCVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAH
                     EDTAIADIPTPDKSELQAAEQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIK
                     RAGGIAVVKLDQNTLQVIIGTKVIALRRDMDNYMGIY"
     gene            1464..>1811
                     /locus_tag="J1Z28_004503"
     CDS             1464..>1811
                     /locus_tag="J1Z28_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-cystathionase"
                     /protein_id="HBA7719248.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDILPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNILV"
BASE COUNT          463 a          351 c          429 g          568 t
ORIGIN      
        1 gatgatatgg caaagttaat tccatttctg gctattccaa taattaaaac catacttgat
       61 ttcattgtta gtcttggttt gtttgccttt gttaatttac atgtattgtt tgcgatagcg
      121 attcccttag gattattaaa agataaagag gataaagcct atggtgcttt ttctggctta
      181 attggtttta tggcgatgca tctgggaacg aacttttatc ttaaacagca cgacttattg
      241 gtcgttgctg accaaatgtc gacacatggg caaaccatca ttctggggat ccagtcctac
      301 aataccagcg tgttgggggg aattgttgct gggttattag tcgccagcat gtataaaaag
      361 atcgttaatt tacgcattcc tgaatcgtta ggtttttata gcggtccacg tctggtgcct
      421 atcattacac tgagtgtgat gagtggattt ggtctgatca ttccttttat ctggccgccg
      481 tttttcaatc ttttcatgct cattggacac tggatttcaa cttccggtcc tgttggttat
      541 ttcttctatg cagttgccga acgcgtgacg attccttttg gcttaaacca tctggtgacg
      601 tcagttttcc gctttacgcc aatcggcggt tcggctgtga ttggcggcga agaatattac
      661 ggcaccctga acatgtttat ggcatacgtc aaagagaatg cggtcattcc gctggatttg
      721 gcggggaaaa tggagcaggg caaactgatg attcagtatg gtctggctgg tgccgcgctg
      781 gcgatgtatc gcactgctca tgctcaaaac agaaaggcta tcaaagcatt gcttatttcc
      841 ggggtgctta cggtgattat tggcggcgtc agcgaaccga ttgagtttct gttcttattt
      901 gtcagtccac tgctgtttgt cttccatgcc tttatgaatg gattcgctaa catggttctg
      961 ccatatatgg gcgtgaagat gggatttact ggcgatctga ttcaatttat tagctttggc
     1021 gtattgcgtg gcacaagaac aggttggccg atcgcggtgt gtgtcgaagt ggcctatttc
     1081 ttcatttatt actttgtgtt ccgttggacc attcttaaat ttaacctgat gaccgtaggc
     1141 cgtgaagagt ccagtcctgt cacgctgaac gctcacgaag atacggctat agcggatatc
     1201 ccaactcctg ataaatcaga gctgcaagcg gcggagcaga tggttaaggc acttggtggt
     1261 aaagagaata ttaagtcact ggataattgc gtaactcgtt tacgtttaac aatcgcagat
     1321 atgggattga ttgacgaagt tgcaataaaa agagctggcg ggattgcggt tgttaaactt
     1381 gatcaaaata ccctacaagt cattatcggc actaaagtca tcgccctgcg tcgggatatg
     1441 gataactata tggggatata ctgatggatg tttttaatac tcctgtttcc cgcaaaggaa
     1501 catattgtac ccaatgggac ttttgcgagg acagatttgg tgtaaaagat atactaccat
     1561 tctctatttc agatatggat cttccgatac cagacgctat caccagagcg ttaaaaaaac
     1621 gcctggaaca cccgatttta gggtacagtc gttggcaaca tggtgaatat ctgaatgcga
     1681 tagtaaactg gtattatcaa caatatcaaa cagatattaa acctgaatgg ataacctata
     1741 gccccagtgt gatgtactct attgcaaaag caatagagtt attgacatcg catggtgata
     1801 atattctcgt t
//
LOCUS       DADSZD010000334         1756 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.336, whole genome shotgun sequence.
ACCESSION   DADSZD010000334 DADSZD010000000
VERSION     DADSZD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.336"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..669
                     /locus_tag="J1Z28_004504"
     CDS             <1..669
                     /locus_tag="J1Z28_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7719249.1"
                     /translation="RTPEGERYFPCVNISGEPEDYFRMAPEDWLQAEMQGEIVALVHS
                     HPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLSGRRFEHGVTDCYTLFRD
                     AYHLAGIEMPDFHRGDDWWRNGQNLYLDNLEATGLYQVPLSAAQPGDVLMCCFGSSVP
                     NHAAIYCGDSELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWHASAFTGIYNDLAAA
                     STFE"
     gene            567..1214
                     /locus_tag="J1Z28_004505"
     CDS             567..1214
                     /locus_tag="J1Z28_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA7719250.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQNPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            1275..>1756
                     /locus_tag="J1Z28_004506"
     CDS             1275..>1756
                     /locus_tag="J1Z28_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7719251.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNANISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     S"
BASE COUNT          368 a          429 c          594 g          365 t
ORIGIN      
        1 agaacgccgg agggggaaag atattttccc tgcgtgaata tctccggtga gccggaggat
       61 tatttccgga tggctccgga ggactggctg caggcagaaa tgcagggtga gattgtggcg
      121 ctggtccaca gccaccccgg tggtctgccc tggctgagtg aggccgaccg gcggctgcag
      181 gtgcagagtg atttgccgtg gtggctggtt tgccgggggg cgattcacaa gttccgctgt
      241 gtgccacatc tttccgggcg gcgctttgag cacggggtga cggactgtta cacgctgttc
      301 cgggatgctt accatctggc ggggattgag atgccggatt ttcatcgcgg ggatgactgg
      361 tggcgtaacg gtcagaatct ctatctggat aatctggagg ccacagggct gtatcaggtg
      421 ccgttgtcag cggcgcagcc gggcgatgtg ctgatgtgct gttttggttc atcggtgccg
      481 aatcatgccg ccatttactg tggtgacagc gagctgctgc accatattcc tgaacaactg
      541 agcaaacgag agaggtacac cgacaaatgg cagcgacgca cacactccct ctggcgtcac
      601 cgggcatggc acgcatctgc ctttacgggg atttacaacg atttggccgc cgcatcgacc
      661 ttcgagtgaa aacggggtcc gaagccatcc gggcgctggc catgcagaac ccggcgtttc
      721 gtcagaaact gagcgacggc tggtaccagg tacgcattgc cgggcgtgat gcaggtgaaa
      781 ccgaattgtc tgcccgtctt aatgagccgc tggcaaatgg tgccgtgatc cacatcgtgc
      841 cgcgtctggc gggagctaaa agtggcggtg tgtttcaggt ggtgctgggg gcggcgctga
      901 ttgcggtggc atggtggaac cctgtgggct ggctgggtgc cgcggctgta tcgggcatgt
      961 atgcggcagg ggccagtatg atcctgggcg gagtggcgca gatgctggca ccgaaagcca
     1021 ggacgcccac ggcagcaagt acagataacg gcaaacagaa cacctatttc tcgtcactgg
     1081 ataacatggt tgcccagggc aatgttctgc ctgttctgta cggggaaatg cgcgtggggt
     1141 cacgcgtggt ttctcaggag atcagcacgg cagacgaagg ggatggtggt caggttgtgg
     1201 tgattggtcg ctgatgcaaa atgttttatg tgaaaccgcc tccgggcggt tttatcgttt
     1261 atggagcatg acgaatgggt aaaggcagca gtaaggggca taccccgcgc gaagcgaagg
     1321 acaacctgaa gtccacgcag ctgctgagtg tgatcgatgc catcagcgaa gggccggttg
     1381 aaggtccggt ggatggatta aaaagcgtgc tgctgaacag tacgccggtg ctggacagtg
     1441 aggggaatgc caatatctcc ggcgtcacgg tggtgtttcg ggcaggtgag caggagcaga
     1501 caccgccgga gggatttgaa tcctccggct ccgagacggt gctcggtaca gaagtgaaat
     1561 atgacacgcc gatcacccgg accatcacgt cggcaaacat tgaccgtctg cgttttactt
     1621 tcggcgtgca ggcactggtg gaaaccacct caaaggggga caggaatcca tcggaagtcc
     1681 gcctgctggt tcagatacaa cgtaacggtg gctgggtgac ggaaaaagac atcaccatta
     1741 agggtaaaac cacttc
//
LOCUS       DADSZD010000335         1730 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.337, whole genome shotgun sequence.
ACCESSION   DADSZD010000335 DADSZD010000000
VERSION     DADSZD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.337"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(43..804)
                     /gene="kduD"
                     /locus_tag="J1Z28_004507"
     CDS             complement(43..804)
                     /gene="kduD"
                     /locus_tag="J1Z28_004507"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBA7719252.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(834..1670)
                     /gene="kduI"
                     /locus_tag="J1Z28_004508"
     CDS             complement(834..1670)
                     /gene="kduI"
                     /locus_tag="J1Z28_004508"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBA7719253.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
BASE COUNT          450 a          472 c          390 g          418 t
ORIGIN      
        1 aaccctgcca tgcggcaggg tcataaaagt aagaggaatg aattaacgcg ccagccaacc
       61 gccatccaca gcaatggtat aaccatttac ataatctgaa gcgctggagg caagaaacac
      121 taccggcccc atcaggtcac tcggcagtcc ccaacggcca gctggaatgc ggtcgagaat
      181 ttccgcgcta cgttgttcat ctgcccgcag ttgttgagta ttgttggtcg ccatgtaacc
      241 cggagctatc gcattaacat taatgttgtg tttagcccat tcattcgcca tcaatcgcgt
      301 cacgcccatc acgccgcttt ttgatgcggt ataagaaggc acacggatcc cgccctggaa
      361 ggagagcatt gacgcgatat tgataatctt gccgccattg ccttgcgcga taaagtgttt
      421 cgccgctgcc tgagacatga agaatacgct cttgatattc aggttcatga catcgtccca
      481 gtctttttcg ctgaattcga gagcgtcttc gcggcgaatc aatccggcgt tattcaccag
      541 gatatcaata tgaccaaact cagctaccgc gcgatccagc agtgctggaa tgccatcaat
      601 ctttcgcaga tcggcggtca ggcttaaaaa acgacgcccc agcgccgtga cctgcttgat
      661 ggtttcagtc ggttcaacga tgttaatgcc aacaatgtca cagcccgctt gcgccagccc
      721 caacgccatc ccctggccca gcccagtatc acaaccagtg acgaccgcaa ctttaccttc
      781 gagagaaaat gcacttaaaa tcataacaat accttattcg tttatgccca caactagcgc
      841 aaatctttaa cggccacatg gtccatatca tcaaaaacct ggttttcacc gaccatgccc
      901 cagataaagg tataagcttt ggttccgacg ccggaatgga tcgaccagct cggggagatc
      961 accgcctgct cgttatgcat cacaatgtga cgcgtttctt gcggttgccc catcatgtgg
     1021 aaaacgcagg cgtcatcatc catattgaaa tagaaataaa cttccatccg gcgctcgtgg
     1081 gtgtgacacg gcatggtgtt ccacaagtta cctggagcca gctccgtcag ccccatactc
     1141 aattggcagg tttccagcac atccgggaca aaatatttgt taatcgtgcg acggttactg
     1201 gtgaggttat cgcctaacgt gactggagat acttcgtccg gtgtgacttt tttggtggga
     1261 tacgtcgtat gcgcgggtgc gcaattgtaa taaaacttcg ccggagtggc ggtatcaata
     1321 ctggcaaaga caacttcttt tgcaccttta ccaacataca gggcgtcgcg gtgaccgatt
     1381 tcatagcatt gaccatcgac agtaatcgta ccggcaccgc caatattgat aacacctaac
     1441 tcgcgacgtt caaggaaata gcttacgccg agttgtttac caacttcccc gccaacggaa
     1501 accgttttag ttaccggcat aatgccgcca acaataatac ggtcaatgtg gctgtaaacc
     1561 atggtgtact catcggcgac aaataccttt tcaaccaaaa attcattgcg cagcccctgg
     1621 gtatccagcg tttttgcgtg cgcactgtgg atgctctgtc ttacgtccac atcaacctcc
     1681 gataatcagt gaagcgtgaa aatagaaaac aataataaaa cactgtttcg
//
LOCUS       DADSZD010000336         1728 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.338, whole genome shotgun sequence.
ACCESSION   DADSZD010000336 DADSZD010000000
VERSION     DADSZD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.338"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            91..471
                     /locus_tag="J1Z28_004509"
                     /pseudo
     CDS             91..471
                     /locus_tag="J1Z28_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1318..1728
                     /locus_tag="J1Z28_004510"
                     /pseudo
     CDS             1318..1728
                     /locus_tag="J1Z28_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          493 a          347 c          425 g          463 t
ORIGIN      
        1 caagtaattg ttggcgataa tatcggtctt ggctcagccc cgcgtgattg tcctgatatt
       61 tccggcaacc catcagggta tatcggattt atgcgcatta tgagtaatgc aaaaggcttt
      121 ccatcgattg catccggtga aagcagtctt acggggttta ttagtcaggt agatggaact
      181 ccagcgtata caggtgtatt tcagggatgg gctacgcgct cgctttatac ttatcgctgg
      241 aatccgacaa caggcccgca atggacacgc cacgctcgca aaaatgaagt ggaccgtctt
      301 gaccaatgga atagcgaaac atggttatat aaccatgata aatccatgcg cctgggttta
      361 accggatcaa catgggggtg ttacagcgac acacaaaaaa aatggatacc gcttgatgtt
      421 tctcatggtg gcactggcgc aaatagcctt gatgacgcca aaactaattt gcaaatccct
      481 gagggtggat taacaaaagc gatgaccctt aatgcccctg gtggtgcagt ggatggcaaa
      541 tactaccctg tcataataga cacatccgca atgaatggga gcggtgctat gttatgtcct
      601 gttgaaataa aaacagcagg aggtccggca tcaaatccgc ttaatagtaa ctcatttttt
      661 gggttcattc gatgtggtgg ctggagtgac gttcgcgatg tggcttacgg gtcatttgtt
      721 tgctatgaaa aaaatgaact cgggatactt tgcattaaag cgtcgcaaag agattatgcg
      781 cagcatgtag ctttctacat ccataaaagc gcttttcctg tcgttgtgca aactggctac
      841 aaggcgaaag ttaaggttcc aacagaagac tacgtaattg gtgacaacgg ggttagatat
      901 aagtttggtg tatcaacgtc aacggaggat aacgacaaga actttgttaa aaatgttctt
      961 gacttcactg gcggtgaaca tggcttctac agcaaccttc cgttcagaca aggggtgtca
     1021 gaaaggattg cattaacaaa tggcctgagt tttgacaaaa attttaccgt cactattcct
     1081 atatttgtaa acaatggtca gattaaagca acacaatctt tcaccgcgta tggtacggac
     1141 gtttcaaaca gaacatttgt ttctcaaaga ttacaatccg aaggaggtcc ggttgtcgat
     1201 caaactgaac taagggccgc taacgcttct ggtgaaattg ttgtcaggga tataaataat
     1261 tcagggaaca gtaagttctt taattttaac cttgatggta ccttcagttc ttatcaggga
     1321 ttggttgttc acacagggca aaactggagc actcaacata cagagaatgt aaataagttt
     1381 aagccaatag cagggagcgc aggcggtcct gacggaacaa tggttgttgg cggattccat
     1441 gctcaattta gcggtaatta cgtcacacaa ttcgccggtc gcaactccaa attttgggca
     1501 agaagctttg aggctggggt tgataaggga tggaaacgac tattaacagt agacgatctc
     1561 aattcatcta ccgatcttgc tgtcaggtca ttaaccacat ctaacccggt aaaatctggc
     1621 ggagggcgga ttgatgtcct tggaagcacg tcagactata gcaaaatgga ttgctttgta
     1681 cgtgggtttg atagcaccgg taattctctc gcgtgggcgt tgggttca
//
LOCUS       DADSZD010000337         1712 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.339, whole genome shotgun sequence.
ACCESSION   DADSZD010000337 DADSZD010000000
VERSION     DADSZD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.339"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            280..>1712
                     /locus_tag="J1Z28_004511"
     CDS             280..>1712
                     /locus_tag="J1Z28_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug DMT transporter permease"
                     /protein_id="HBA7719254.1"
                     /translation="MNDLRILIARLGWPATSARWWTIQELAARLGEPDTKAETESLLL
                     QLLRSRKLEAEVVEVLCIFWMAIKEFNYFPTTLLAENILLPSPLSALLMKDFGLSILL
                     VNTDLELAPEDFIIPDDFDSVQGTDLPRIFHTTISKLETFTRCPFIRQMAFEWTKNRT
                     AYPNAQYQGDAGYFIRPLGEGFAPHFSSRTALRVISAYLRTLAVARHIWRIPMELTEQ
                     HSLLALPIHPTLAFLRPNRPEWLSVSTEFKSDSKAIEASLRTFFAQVEAANPGDELIA
                     FSSPISMSMEQCVELSIVRWSQTVSSDKEGADSLTSHLESFWTREQMLSSQAPDPLST
                     TTLITPPTFEQLVNKTCMAWPLAGTLDFGRIGYLQHDLYPSRLFLPTMPGIENSLEVI
                     PHDGRLQAKIKDQVVAELHYWNAGWGPARLWQIQGNCGTALISRGTTYRERNITNTGS
                     LHSFYSWQVRRLHRNGSYGRFSEKITRG"
BASE COUNT          445 a          395 c          401 g          471 t
ORIGIN      
        1 gacttgcaac agactcgacc tggttgccca aaattatccg cagcgctgtg atgaatttgt
       61 gaccgtaaca acctacagca tgtttgatga cccggtgtcg tcacgcttag ccccatcgga
      121 agtcatggtc tatttctacg ttaagcaaaa tcgcatagat gaagctataa aattcacgga
      181 gacgatggtc aattgtgtga ttgaggatac tcgaacgttg cccttggaac caccttgctg
      241 gatgtcagag ctgctcgaac ctgtcggcct ggaggttaaa tgaacgatct tcgcattctt
      301 attgcccgtc ttggatggcc tgctacgtcc gcccggtggt ggactattca ggaacttgcc
      361 gctcgactgg gagagcctga tacgaaagct gaaaccgaat cattgctgct tcaattgctt
      421 cgctcgcgca aacttgaagc tgaggttgtc gaagtattat gtattttctg gatggcaatt
      481 aaagagttta actactttcc tacgactttg ttggcagaga atattcttct accctcacca
      541 ctctcagcat tgttgatgaa agattttggg ttatcgattc tgctcgtgaa tactgatttg
      601 gaactagctc cagaagactt catcattccc gatgactttg atagtgttca aggtacggat
      661 ttgccgagaa tcttccatac aaccattagc aagttggaaa cttttacccg ctgtccgttt
      721 attcgacaaa tggccttcga atggacaaaa aaccggacgg catatccaaa tgcacagtac
      781 caaggggatg caggatattt cattcggccc ttgggagagg gctttgcccc tcatttttcc
      841 tctcgaacag cccttcgcgt aatttccgct tatcttcgca cgttagctgt ggctagacac
      901 atctggagga taccaatgga gttgaccgag caacactctc tcttggcgtt gcctatccat
      961 ccaactttgg cttttcttcg acctaatcgt cccgaatggc tctcagtatc gactgaattc
     1021 aaaagtgata gtaaggcgat cgaggcttca ttgcgaactt tctttgctca agtagaggcg
     1081 gcaaacccag gagacgagct aattgcattt agctcgccca tttcaatgtc aatggaacag
     1141 tgcgtagagt tgtcgattgt tcggtggtca caaactgtaa gtagtgataa ggaaggagcg
     1201 gactcgttga catcccatct tgaatctttc tggacacgtg aacaaatgct ttctagccaa
     1261 gctccggatc cactcagtac aacaacctta ataactccac caacttttga acaactggtg
     1321 aataaaactt gtatggcttg gccattggcg ggaacgctcg attttggtcg gataggatac
     1381 ctacaacatg atttgtatcc atcccggcta tttttaccga cgatgccagg catagaaaat
     1441 tctcttgaag tcatcccgca tgatgggcgt ctacaggcta agataaagga ccaggtcgtt
     1501 gcagagctcc attattggaa tgctggatgg gggcctgcta ggctgtggca gattcaaggt
     1561 aattgtggga cagccctcat atcgcgaggc actacatatc gcgagagaaa cataacaaat
     1621 accgggtcat tgcactcatt ttactcttgg caagtacgta gattacatcg taatggaagt
     1681 tatggcagat tcagtgaaaa aattacgaga gg
//
LOCUS       DADSZD010000338         1700 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.340, whole genome shotgun sequence.
ACCESSION   DADSZD010000338 DADSZD010000000
VERSION     DADSZD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.340"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(403..615)
                     /locus_tag="J1Z28_004512"
                     /pseudo
     CDS             complement(403..615)
                     /locus_tag="J1Z28_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            716..1298
                     /locus_tag="J1Z28_004513"
                     /pseudo
     CDS             716..1298
                     /locus_tag="J1Z28_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          426 a          397 c          419 g          458 t
ORIGIN      
        1 aggtaatggc agatttctgg tgatcgggtc tgagcagtta aaagattatg aatttaatat
       61 ggttcgggtt aacctgtagg ttgctctcct gtttcttaaa tactcgcttt gatgttacgc
      121 taatggaact aggttgacac gtttttagtg cgcgccagca gcgatttaag tgctctgcgt
      181 attttcatgg aaggagatca cccaataacc ccgtaactca ccgatctcct tcacggtaat
      241 acgtatgcac agtatggtga tttgcgcagt tgtgtttcac cggagcgatt tatcctgtag
      301 cgacagtctt gaccgccagg cggcaactgc ataattttaa gccacacttt tacactccgg
      361 tgaaccatcc cggttttcct ggagagtatt ttatctgtga actcaggctg ccagatcatt
      421 gtttccgatg gaggcataat aagctttttc tgtttctgtc ggaggaatat ggcccagcct
      481 tttcagtaat cgtcgattgt tataccagtc cacccacgtt agtgtggcca gtttcacttc
      541 tgtccggttt ttccagctct tacggtgtat tacctccgtt ttgtaaagac cattgatgct
      601 ctcctccatc gcgttgtagt ggcacagtaa atttggccac ctgattaaag gtgatactct
      661 caccacaaca taaaacaaca agaaaacaaa gcgtaccttc tctcctgagt ttaatctgga
      721 atgcgcccaa cttatcgttg ataaaggtta ctcataccgg gaagctactg aagctatgaa
      781 tgttggtttc tctactctgg aggcatgggt atgtcagctc agacgggaac gtcaggatat
      841 cacgccttct gcagtaccac tcacatcaga gcagcaacgt attcgtgagc tggaaaagca
      901 ggtgcgtcgt ctggaggaac aaaatacgat attaaaaaag gctaccgcgc acttgatatc
      961 agactccctg aatagttccc gataatcggg aaactcagag cgcattatcc ggtggtcaca
     1021 ctctgccatg tgttcagggt tcatcgcagt agctacagat actggaaaaa ccgtcctgaa
     1081 aaaccagatg ggctgtatta cacagtcagg tacttgagct acatggcatc agccacggtt
     1141 cggccggagc aagaagcatc gccacaatgg caacccggag aggctaccag atgggacgct
     1201 ggcttgctgg caggctcatg aaagagctgg ggttggtcag ctgtcagcag ccgactcacc
     1261 ggtataaacg tggtggtcat gaacatgttg ctatccctaa aagcaacagc aaacagcgac
     1321 cactggggag ccctgcattg cgggattgta ttgttcagcg ggccatgctg atggcgatgg
     1381 ggccgaggag agtgattttc atacgctctt atatggtttt cgacttgtgc gaaatgtcca
     1441 ctacgcgatc cgcacggtga aactgcaact caccgacttc aggggaaact cggggccgct
     1501 gggtaatctc acataaaagt tcttcggtgt cataaacaac gagagtattt gattccttta
     1561 tggcggcctg gtgcagagct gccctttccc aggacctcca tataattttt gtagcggcag
     1621 tcagtggcac actcagttaa ctactttcac ttcagtgact ttgaatgagt cagggctgcc
     1681 gttaaaggtg ttaatgaagg
//
LOCUS       DADSZD010000339         1692 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.341, whole genome shotgun sequence.
ACCESSION   DADSZD010000339 DADSZD010000000
VERSION     DADSZD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.341"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            337..>1692
                     /locus_tag="J1Z28_004514"
     CDS             337..>1692
                     /locus_tag="J1Z28_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719255.1"
                     /translation="MRIMENATGFPSIASGEGNLTGFISQVDGSPAYTGIFQGWNTRS
                     IYTYRWLTSIGPQWTRHARKDEVSRLFQNESTTRIYGPTNDRFFEIHTNGSWGVYDSS
                     SGQWSPLGLAQGGTGGRTIEQARANLRVMYEQKAGLANTDLNTLTGEYSGFYQQPASA
                     YATEELNYPIGLAGALIVLQTRANTASSCVQVYYPYNNPGITYRRIYEGGSGTWSEWK
                     RDVSTERVEEGKETTYVYSTYSSGAPRLQVSKSGLWGCHNGTGWLPLAVGQGGTGATT
                     VEDARNNLSLGESSAVKFKNLTLTEALDTTLGLLTKTGRDWNTQHTDNINKFIPIAGS
                     TNGPAGSMVLGGIHVQFSKNYAVQFGGRNSGFWGRTVENGTTQEWKKLLTVDDLNSST
                     DLAVRSLTTSNPLKSGGGRIDVLGSTSDYSKMDCFVRGFDSTGNSLAWALGSSVGVSK
                     ML"
BASE COUNT          502 a          366 c          420 g          404 t
ORIGIN      
        1 tccgcgaagg ccgcaaaaac gtctgagacg aatgcggata acagcgcaca ggcggcagca
       61 gactcacaaa ctgcatcagc aaactccgcg acagcagtca aaaaatcaga aaccaacgcg
      121 aaaaatagcg aggcagcagc aaagagcagc gaaaccaacg ctaaagccag cgaaactaat
      181 gctaaatcca gtgaaacaaa cgcggcgaaa tctgcggcgg acgcacttaa ttatcgcaac
      241 caggcccagg gtattgttgg tactaacatc ggtttgggat catcaccgcg tgattgctct
      301 gacatctcag gaaatccatc aggttatatt ggctttatgc gcatcatgga aaatgccacg
      361 ggttttcctt cgattgcatc gggtgagggt aatttaactg gctttataag tcaggttgac
      421 ggatcgccag cttatacagg tattttccag gggtggaaca ccagatccat ttacacctat
      481 cgctggttaa ctagtatagg cccgcaatgg acacgccacg ctcgcaaaga tgaagtcagt
      541 cgattatttc aaaacgaatc gacgactcgt atatatggcc caactaacga tcgtttcttt
      601 gaaatccata ctaacgggag ttggggggtt tatgattctt cttctggaca atggtctccg
      661 ttaggtttag cccagggcgg tactggtgga agaactatcg agcaagcacg tgcgaacttg
      721 cgggtaatgt atgagcaaaa agctggtctt gctaatactg acctaaacac ccttaccggt
      781 gaatattctg gtttctatca acaaccagcg agcgcttacg caacagaaga attaaattac
      841 ccaatcggtc tggcgggcgc tttaatagtg ctccaaacga gagccaacac tgcttcttcc
      901 tgcgttcagg tgtactaccc ttataataat ccgggaatta cttatagacg aatatatgaa
      961 ggaggtagcg gtacctggtc tgaatggaag agagatgtat caacagaaag ggttgaagag
     1021 ggaaaagaaa caacttacgt atattctacg tattcttcag gcgcaccacg cttacaggtt
     1081 tccaaatctg gtttgtgggg ttgtcataat ggcactggct ggttgccatt agctgttggg
     1141 caaggaggta caggtgcgac aacagtagaa gatgcgcgaa acaacttaag tcttggcgaa
     1201 agtagcgcag ttaaatttaa aaaccttact ttaaccgaag cgctcgacac gacattagga
     1261 ctgcttacaa aaacaggacg agactggaac acgcagcata ctgataacat taataaattt
     1321 ataccaattg caggcagtac aaacggcccg gcaggttcta tggttcttgg cggcattcat
     1381 gttcaattta gtaaaaatta tgctgtgcag ttcggaggcc gcaattccgg tttttgggga
     1441 agaacagttg aaaacggaac gacgcaggaa tggaagaaat tactaacagt agacgatcta
     1501 aattcatcca ccgatcttgc tgtcaggtca ttaaccacat ctaacccgtt aaaatctggc
     1561 ggagggcgga ttgatgtcct tggaagcacg tcagactata gcaaaatgga ttgctttgta
     1621 cgtgggtttg atagcaccgg taattctctc gcgtgggcgt tgggttcatc agtcggcgta
     1681 agtaagatgc tg
//
LOCUS       DADSZD010000340         1656 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.342, whole genome shotgun sequence.
ACCESSION   DADSZD010000340 DADSZD010000000
VERSION     DADSZD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.342"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>1656
                     /gene="tssI"
                     /locus_tag="J1Z28_004515"
     CDS             <1..>1656
                     /gene="tssI"
                     /locus_tag="J1Z28_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBA7719256.1"
                     /translation="YSIKLKTPDILNLDYVSPAANLQLKPMVGKDLCVNIELDGGGKR
                     YISGLVTAARVVGHQGRSVVYELRLEPWLKTLTHTSDYKAFQNKNVVNILDEVLDEYP
                     WPVEKRLVENYPTRAWQVQYGETDFDFIQRLMQEWGIYWWFEHNENSHTLVLADAINV
                     HKACADSPLVCYYQKGLKLDKEFIHTITANESLRSGQWVLNDFDFMKPRSLLKSTVAN
                     PRETGLAEYEHYEWPGDYFTKSEGEMLTRIRMEEQRSPGSRVQGSGNIRTLMTGFTFT
                     LENYPTAEVNREYLLVQTTLFIQDNAQHSGQEQHFSYSTSFELHPTSEVYRPQRTLSK
                     PHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDENSSCWVRVSYPWAGKGF
                     GMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGLPGAATQSGFYSHTIGGG
                     PTNANALRFEDKPGGEEVWLHAEKDQRIEVNNNESHWVGNNRLKVIDKTETAIIGEKR
                     SLTVQMDDTSLAGQNKTIQAVQNLRLAAGDSIILSCGKTILQM"
BASE COUNT          465 a          396 c          446 g          349 t
ORIGIN      
        1 cttattctat taaattaaaa actcctgaca tcctgaacct ggactatgtt tcccccgcag
       61 ccaacctgca gctcaaaccg atggtgggca aagatctgtg cgtcaatatc gaattggatg
      121 gcggcggaaa acgttatatc agcgggctgg tgacggcggc gagggtggtc ggccatcaag
      181 gacgctcggt ggtgtatgaa ctgcgactcg aaccgtggtt aaaaacactg acccacacta
      241 gtgactacaa agcgtttcag aataaaaacg tggtgaacat cctggatgag gtactggatg
      301 agtacccttg gccagtggaa aaacgattgg tagaaaacta tccgactcgc gcctggcagg
      361 tgcagtacgg tgaaacagat ttcgatttta ttcagcgcct gatgcaggag tggggtatct
      421 actggtggtt tgagcacaac gagaacagtc acacgttagt gctggctgat gccattaatg
      481 tccacaaggc atgcgcagat tcgccgctgg tgtgttatta ccagaaaggg ctgaaactgg
      541 acaaggagtt tatccatacc atcacagcaa atgaaagcct gcgcagcgga cagtgggtgc
      601 tgaatgattt cgactttatg aagccgcgtt cactgctgaa gagcactgtg gctaatccgc
      661 gtgaaaccgg ccttgctgaa tatgagcact acgaatggcc gggagactac ttcacaaaga
      721 gtgaaggtga aatgctgaca cgcatccgaa tggaagaaca gcgtagcccc ggtagtcggg
      781 tacagggcag cggcaatatt cgtaccctga tgacaggttt taccttcacc ctggaaaact
      841 atccgacagc cgaagtgaac cgggaatacc tgctggtaca gaccacgcta tttatacagg
      901 acaatgcgca acacagcggc caggagcaac atttctctta ctcaaccagc tttgaactgc
      961 atcccaccag cgaagtttac cgtccacaac gaaccctgag caaaccccac actaaaggcc
     1021 cgcaaagtgc catcgtcacc ggcccggcag ggcaggaaat ctggacagat aaatatggcc
     1081 gggtgaaagt tcagtttggc tgggatcgtt acggtaagaa tgacgaaaac agttcctgct
     1141 gggtccgtgt cagttatccg tgggcgggta aaggattcgg gatgatccag atcccgcgca
     1201 tcggccagga agtgctggtg gacttcaaaa acggcgaccc ggatttaccg attattgtgg
     1261 ggcgcaccta caaccaggac acgatgccgc cgtggggact accgggcgca gcgacgcaaa
     1321 gcgggtttta cagccacact atcggcggcg gcccaaccaa tgccaacgca ctacgttttg
     1381 aggataaacc tggcggtgaa gaggtctggc tacatgccga aaaagatcag cggattgaag
     1441 ttaataataa tgaaagccac tgggtgggca ataatcgcct taaagtcatt gataaaacag
     1501 aaacagcgat aattggtgag aaacgttctc ttacagttca gatggatgat acctcccttg
     1561 ccggacaaaa taaaacgatt caggccgtac agaatttacg ccttgctgcg ggagattcca
     1621 taattcttag ttgtgggaaa actatcctgc aaatga
//
LOCUS       DADSZD010000341         1598 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.343, whole genome shotgun sequence.
ACCESSION   DADSZD010000341 DADSZD010000000
VERSION     DADSZD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.343"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(33..668)
                     /gene="msrQ"
                     /locus_tag="J1Z28_004516"
     CDS             complement(33..668)
                     /gene="msrQ"
                     /locus_tag="J1Z28_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBA7719257.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGELGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            complement(669..>1598)
                     /gene="msrP"
                     /locus_tag="J1Z28_004517"
     CDS             complement(669..>1598)
                     /gene="msrP"
                     /locus_tag="J1Z28_004517"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBA7719258.1"
                     /translation="LKALGISAAAFSLPHAAHADLLSWFKGNDRPPAPAGKPLEFSKP
                     AAWQNNLPLTPADKVSGYNNFYEFGLDKADPAANAGSLKTDPWTLKISGEVAKPLTLD
                     HDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFPLHKLLALAEPTSNAKYVAFETIYAP
                     EQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLTLMTVGVYGKALPPQNGAPVRLIVPW
                     KYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYGFYANVNPHVDHPRWSQATERFIGSG
                     GILDVQRQPTLLFNGYADQVASLYRGLDLRENF"
BASE COUNT          414 a          402 c          413 g          369 t
ORIGIN      
        1 gggataacgc gaagtttgtc ggcaatatga agttatagag ataatttgtt gtgggcctgc
       61 ttgcgtaacc ggttgaaaag ggaaagcaac ttcttatacc gtaaggctaa aagcagtaca
      121 gccagcccag cgtagatgag tggctgcggt gaaataatct tcactgacca cagataatgt
      181 attggggcca ggatcgcgac aagatagacg aagttatgca actgttgcca gtgtttgcct
      241 agttttcgct gcatcgactg ggttgaagtg aacgctaaag caagcaaaat aacccagctg
      301 ataattccta acgttaaata aggccgggta attaactcct tacctaataa cgccagatta
      361 ttcacgccca actccagcaa tgcgtaactg gttaaatgca gtgtcgccca ggcaaagcac
      421 cataagccta acaggcggcg agtgcgtatc aataacggct gttttgcgta gcgtgccaga
      481 ggtgtgatta acaaggccgc aagcaaaaat ttcagtgcag tgcgaccggt aaaatgctga
      541 atatctttca ccggatcggc acccagttca ccgtgattga tcgcccagac cagccagaga
      601 aacggcaaca atccggcaag atgcaggcaa actttcagcc atatcacctg ttttgctgtc
      661 agacgcattt agaaattctc ccgcaaatcc aggccacgat acagcgatgc cacctggtcg
      721 gcgtaaccat taaacagtag tgttggctgg cgctgaacat cgagaatgcc gcctgaacca
      781 ataaatcgtt cggtagcctg tgaccagcgc gggtgatcaa catgcggatt aacgttggcg
      841 taaaaaccgt attcgtcagg cgctgccaga ttccaggtgg ttggcggacg ctcgcgggtc
      901 agcttaatac tgacgatcga tttaatccct ttaaagccat atttccacgg cacaatcagt
      961 cgcaccggcg cgccattttg cggtggtaac gccttgccat aaacacctac ggtcatcagt
     1021 gtgagcggat gcattgcttc gtcgagacgc aatccttcga cataaggata tttcagcccg
     1081 ccgccgataa agcggtcctg ctggccaggc atctgttccg gcgcataaat cgtttcgaaa
     1141 gcgacatact tcgcattgct ggtgggttcg gcaagcgcca gcaatttgtg tagcggaaaa
     1201 ccaatccacg gcaccaccat cgaccacgct tccacgcagc gcatacgata aatacgctct
     1261 tccagcggga agcgacgggt taaatcatca tgatcgaggg tcaatggttt tgccacttcg
     1321 ccgctgattt tcagtgtcca tggatcggtt ttcaggctac cagcattagc cgcgggatcg
     1381 gctttatcca gcccgaattc atagaagttg ttataaccgg agactttatc tgctggcgtc
     1441 agtggcaggt tattttgcca ggcggcaggc ttgctgaact ccagcggttt tcctgcgggg
     1501 gcgggcggac gatcgttccc tttaaaccag ctaagcagat cggcatgcgc agcgtgaggc
     1561 aaagaaaatg cagctgcgct gatgcccagt gcttttaa
//
LOCUS       DADSZD010000342         1570 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.344, whole genome shotgun sequence.
ACCESSION   DADSZD010000342 DADSZD010000000
VERSION     DADSZD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.344"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            29..289
                     /locus_tag="J1Z28_004518"
     CDS             29..289
                     /locus_tag="J1Z28_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001488293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719259.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSQGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            286..876
                     /locus_tag="J1Z28_004519"
     CDS             286..876
                     /locus_tag="J1Z28_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719260.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVARPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVHLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDSLEVAQWLQACHDAGMEVRVQINKSTAP"
     gene            complement(896..1243)
                     /locus_tag="J1Z28_004520"
     CDS             complement(896..1243)
                     /locus_tag="J1Z28_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719261.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          380 a          404 c          448 g          338 t
ORIGIN      
        1 cagaacatta tcagatggag cgtgcaggat ggatcaggaa atgacgtttt cactcagcta
       61 tgaacaactg acccggtttg cggaaaaacg catccgggaa tgcaatctgg acagccaggg
      121 cgtaacttat ctctgcgagt cagccaaagc cggagctgtg ctgatcttct ggcatgaact
      181 ggccataaat ggctatacct caatgaatgc cataaagaga caggagatca ttgacgccga
      241 ccatcagcgc ctcagaaagc tcatctggcc ggaggatgac tggaaatgac tcagagccgc
      301 cgaccgtcgc cactgcagcg gagagtgctg attgtgctgg ctgcccttga tgaaaaacgg
      361 ccaggccccg tgctgacgcg ggacatagaa cgggtgctgg agcgaagcgg ggaagccccg
      421 gtctacgggc caaacctgcg cgcctcctgc cgtcgtctgg aggatgccgg ctggctgcgt
      481 accctgcgcg ctccgaacct gcagctggca gtggagctga cggacgccgg ccgtgccgtg
      541 gcacggcctc tgctgctggc agaacaggac cgcctccggg cagaacagcg tgcggcagaa
      601 gtcgtcgtac tgccgctggt cccggcagca ggtctgcccg caggcggcac ttctgccact
      661 gatctggcgg tgcatctgaa cggcataacg tatcaggcct gccgtgggga ttttgtggtg
      721 cgtctcgacg gcagcacctg tctgcagtta tggaataaag aaggcagagt ggtacgccgg
      781 gagggcgatt cgctggaagt ggcgcagtgg ctgcaggcct gtcacgatgc aggaatggag
      841 gttcgcgtac agataaataa aagtaccgct ccgtaagaac acgcctgtgc acggtttaca
      901 catcatccca tggtggcagc cggttaactt tcctgcgcca ccagtggaaa gaggccatgc
      961 acagtccgaa caaaaaacca cccgtgatac ttataataat ggcctcacct gctaccattc
     1021 ctgacggccc ccagtagata aaccacattg cacatcccca ggaaatgccc cataagcccc
     1081 ccgtcagcac cgtgacctgc cagaacggca taaagggtaa tggcggaagc cggataccca
     1141 gccgccagag aatacgcaga agaggagggg cataattgct ccgccacatc tttttgctgt
     1201 ccatcagggc aatggcccgg gcttttttct gctcaaaggt cacaggagca cctccgttcc
     1261 atgatgtaaa cgtgacacag cttattgtta ttgattatta ttatcatgct gtgattatgt
     1321 tgtcatgcac cagtacactg acaaaagaaa gggagaacag gaggctggct gatgaacagg
     1381 aaatattatc ttaataatat gtggtgggga tggtttatgg gtgggtatac tctgtatatg
     1441 tcatgggatt atgagtttaa atacagattg ctgttctggt gtatttctct ctgcagaatg
     1501 gttttgtatc cggttgccag atggtttatt gaaaatactg ccctgaaatt tacccggcct
     1561 catttctgga
//
LOCUS       DADSZD010000343         1530 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.345, whole genome shotgun sequence.
ACCESSION   DADSZD010000343 DADSZD010000000
VERSION     DADSZD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.345"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            40..>1530
                     /locus_tag="J1Z28_004521"
     rRNA            40..>1530
                     /locus_tag="J1Z28_004521"
                     /product="23S ribosomal RNA"
BASE COUNT          417 a          319 c          486 g          308 t
ORIGIN      
        1 attttcgcaa cacgatgatg aatcgtaaga aacatcttcg ggttgtgagg ttaagcgact
       61 aagcgtacac ggtggatgcc ctggcagtca gaggcgatga aggacgtgct aatctgcgat
      121 aagcgtcggt aaggtgatat gaaccgttat aaccggcgat ttccgaatgg ggaaacccag
      181 tgtgtttcga cacactatca ttaactgaat ccataggtta atgaggcgaa ccgggggaac
      241 tgaaacatct aagtaccccg aggaaaagaa atcaaccgag attcccccag tagcggcgag
      301 cgaacgggga ggagcccaga gtctgaatca gtgtgtgtgt tagtggaagc gtctggaaag
      361 gcgtgcgata cagggtgaca gccccgtaca caaaaatgca catattgtga gctcgatgag
      421 tagggcggga cacgtggtat cctgtctgaa tatgggggga ccatcctcca aggctaaata
      481 ctcctgactg accgatagtg aaccagtacc gtgagggaaa ggcgaaaaga accccggcga
      541 ggggagtgaa aaagaacctg aaaccgtgta cgtacaagca gtgggagcct cttaatgggg
      601 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      661 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      721 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      781 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      841 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      901 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaaccc gatgcaaact
      961 gcgaataccg gagaatgtta tcacgggaga cacacggcgg gtgctaacgt ccgtcgtgaa
     1021 gagggaaaca acccagaccg ccagctaagg tcccaaagtc atggttaagt gggaaacgat
     1081 gtgggaaggc ccagacagcc aggatgttgg cttagaagca gccatcattt aaagaaagcg
     1141 taatagctca ctggtcgagt cggcctgcgc ggaagatgta acggggctaa accatgcacc
     1201 gaagctgcgg cagcgacact atgtgttgtt gggtagggga gcgttctgta agcctgtgaa
     1261 ggtggcctgt gagggttgct ggaggtatca gaagtgcgaa tgctgacata agtaacgata
     1321 aagcgggtga aaagcccgct cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg
     1381 cagggtgagt cgacccctaa ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat
     1441 attcctgtac ttggtgttac tgcgaagggg ggacggagaa ggctatgttg gccgggcgac
     1501 ggttgtcccg gtttaagcgt gtaggctggt
//
LOCUS       DADSZD010000344         1505 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.346, whole genome shotgun sequence.
ACCESSION   DADSZD010000344 DADSZD010000000
VERSION     DADSZD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.346"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(21..1346)
                     /locus_tag="J1Z28_004522"
     CDS             complement(21..1346)
                     /locus_tag="J1Z28_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBA7719262.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTANNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT          412 a          365 c          296 g          432 t
ORIGIN      
        1 tctggcgtct gattttgagt ttatttgact aatgaaaata gatcattgag tgattcgctc
       61 atcgacggat gagtaaatat ctgatcgcgt aatatgctat aaggcagccc ggcatccatc
      121 accattttca ctatattgat catctcgtgg gagtcaacac acagcagtga tgcccctaat
      181 atacgttggg ttttattatc aacaatcgct tttaataccc cacgagtatc attcatcact
      241 ctggcacgcg gaattgcagc tacaggcaat gtcaccacct gaatatcagc accactctct
      301 ctggcctgtt cttctgtcat accaaccctg gacagtggcg gtgtcatgaa tacggaataa
      361 ggcacatttt tccgatcatc agtactacgt ctgccttctc ccagcaactc atcacgcaca
      421 atgcggtaat catccagtga aatgtaagta aattgcagcc cgccggtaac atctcccatc
      481 gcccaaatat tgtttgcggt ggtatgtaat tgcttgtcaa cgacaattgc cccacgttcg
      541 tttactgcga tacctgcatt ttctggatgt aacgaagcgg tagccggttg acgaccggaa
      601 gctattaaca gtgcatccac cgccagttgg gcgtgctcgc tatgcacttg cacttgattt
      661 tcatggtgac tgattcgctc cacatgggca ttgaggatta tatcgacacc ctgatcgcgt
      721 aaaatcgtcg cgatattatc agcaatatcc cgatcttccc gaggcaaaaa cagcgaagct
      781 gcctctaaaa tggttacttt gctgccaaaa ttagcgaaca tagaggcgaa ctcaacgcca
      841 atatatccgc cgcccaaaat acctaaatgc ccaggcaatt cttttagatt aagtaatccg
      901 gtgctgtcat atacaccagg cgtggtggta attccaggaa ttggcggaac cacggcttgt
      961 gcaccggtat taataaaaat tttctcgcca tgaatctcca gatttccccc aggccgatga
     1021 acacgcaggc tatgattatt gataaactcc gcctggccgt cgatcacgtc gatattgggc
     1081 atatccgcaa gattatgaaa attcttatta cgtaaaaaat taaccacttc atttttacgc
     1141 tgtatggcac ggacaaaatc tgtgtgctgc tgtgcgtcat gaaccaatgt tttggttggg
     1201 atacagccga tattaataca ggtcccgcca tacattgcat ttgattgttc gatgagagcc
     1261 acacgccaac ctgcttttgc cagcgtgacg gctaatgttt ttccagcctt gccaaaacca
     1321 ataataactg cctgatattt attcatgatg atttcctgtc gaaacgcgtt catttgacat
     1381 catgactata gacgcaaaag agcaaataac atttcttcac aacgcttgat attttgtctt
     1441 atcgtctggt attttctttt tcttaatcag aatcacaaaa caaaaatcat gattaactat
     1501 ttgat
//
LOCUS       DADSZD010000345         1492 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.347, whole genome shotgun sequence.
ACCESSION   DADSZD010000345 DADSZD010000000
VERSION     DADSZD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1492
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.347"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(50..1291)
                     /locus_tag="J1Z28_004523"
     CDS             complement(50..1291)
                     /locus_tag="J1Z28_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBA7719263.1"
                     /translation="MARKTKPLTDTEIKAAKPKDADYQLYDGDGLTLLIKSSGSKLWQ
                     FRYYRPLTKQRTKQSFGAYPAVSLSDARKLRAESRVLLAKDIDPQEHQKEQVRNSQEA
                     KTNTFLLVAERWWNVKKASVTVDYADDIWRSLERDVFPAIGDISVTEIKAHTLVKAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAIPSVNIGKAFEKPQKKNMPSIRPDQ
                     LPQLMQTMRTASISMSTRCLFMWQLLTITRPAEAAEARWDEIDFNASEWKIPAARMKM
                     NRDHTVPLSDEALSILEMMKPLSGGREFIFPSRIKPTQPMNSQTVNAALKRAGLGGVL
                     VSHGLRSIASTALNEEGFLPDVIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANL
                     IKAADSIPTVCNEVAGLRLVG"
BASE COUNT          358 a          361 c          358 g          415 t
ORIGIN      
        1 ctcaagtgaa tatcttttct aaggataatc acttgagatt ttaaatccac tacccaacca
       61 atctaagacc tgctacttcg ttacaaacgg taggaatact atctgccgct tttataaggt
      121 ttgcccacca ctgcatcatc ggacgacgtt gctcaaggta atcactgcgg ttgtaagcgc
      181 gacgtacctc atttttgtct acatgagcca gtgctgcttc aatgacatca ggcaggaacc
      241 cttcctcatt gagtgccgta ctagcgatag agcgtaaacc gtgtgatacg agtacgcctc
      301 ctaagccagc acgcttaagt gctgcattaa ctgtttggct gttcattggc tgggttggtt
      361 tgatgcgact gggaaagata aactctcggc caccactgag tggcttcatc atttccagaa
      421 tagaaagagc ctcatcggat agtggaaccg tatggtcccg gttcatcttc attcgagctg
      481 caggaatttt ccattcgcta gcattgaaat cgatctcatc ccatcgagcc tcagcagctt
      541 cggcagggcg ggtgatggtg agaagttgcc acatgaacag gcatctcgtg gacatgctga
      601 tacttgccgt acgcatggtc tgcattagct gcggaagttg atccggccgg atgcttggca
      661 tgtttttctt ttgcggtttc tcgaaagctt ttccgatgtt tacactagga atagcatcaa
      721 tcaggcctgt gttctgcgca taaatcatga cttcgttaat acgctgacaa aggcggcgaa
      781 cagtctctaa tgcacctctg gcctgaaccg gctgtactgc tttaaccaga gtatgagcct
      841 taatctcagt gacactgata tcaccaattg ccgggaaaac atctctctca agcgagcgcc
      901 agatatcgtc ggcatagtcc actgttacgc tggctttctt cacattccac caacgctcgg
      961 caactaacaa gaaggtgttg gttttggcct cttgagaatt cctcacctgt tctttctgat
     1021 gttcctgagg atcaatatct tttgccaata aaactcgaga ttcggctctg agtttacgcg
     1081 catcagaaag tgagacggca gggtaggcac cgaagctctg cttggttcgc tgctttgtca
     1141 gaggtcgata gtaacggaac tgccagagct tactaccact ggacttgatt aacagagtga
     1201 gcccgtcacc atcatacagc tggtaatcgg catctttagg tttggcggct ttgatttccg
     1261 tatcggttaa cggcttggtt tttcttgcca tggggagtct ccatgcgttt aggcccaacg
     1321 aaaacaatag aacttttcgt tgggcctatc aatgggccta aaaggttcgg atttaattag
     1381 ttctcttcag acttcgcggg acaaattgag ggcacaaaaa agcccgcagg gcttgcgccg
     1441 tgcgggcttt taggacttca tcggatgact ctggtaatca ccgatggaga at
//
LOCUS       DADSZD010000346         1490 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.348, whole genome shotgun sequence.
ACCESSION   DADSZD010000346 DADSZD010000000
VERSION     DADSZD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.348"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..120
                     /locus_tag="J1Z28_004524"
     CDS             <1..120
                     /locus_tag="J1Z28_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719264.1"
                     /translation="IITVTLILNYLNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            182..466
                     /locus_tag="J1Z28_004525"
     CDS             182..466
                     /locus_tag="J1Z28_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBA7719265.1"
                     /translation="MADMNRTTKGALLGAGVGLLTGNGVNGVLKGAAVGAGVGAVTEK
                     GRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            470..805
                     /locus_tag="J1Z28_004526"
     CDS             470..805
                     /locus_tag="J1Z28_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBA7719266.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(863..>1490)
                     /locus_tag="J1Z28_004527"
     CDS             complement(863..>1490)
                     /locus_tag="J1Z28_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBA7719267.1"
                     /translation="NVTSESTETPTPVLRPEAGSYVANLAAANTLFVMRLNDRAGETR
                     YIDPVTEQERSSRFWLRQIGGHNAWRDSNGQLRTTSAQAIWMGVDHNVHTEANGSRIE
                     NDANNNIQTRLGFHTFIRTQEKNSGPHGDDFEPFVEMNGIHNSKDFAVSINGVKVEQA
                     GARNLGEMKHGVNGNLNPAASVWGNVGVQLGDNGYNDTAMMVGLKYKF"
BASE COUNT          383 a          331 c          380 g          396 t
ORIGIN      
        1 atcatcacag tcacgctcat tttgaattat ctgaataata gcgagcgttc tttagttaag
       61 cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc ggacatctaa
      121 gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct ccacgccagc
      181 gatggcggat atgaatcgca ccacgaaagg tgcattgcta ggcgcaggcg ttggtttact
      241 tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg gtgttggtgc
      301 agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag tgggtgccgc
      361 cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga ttaaaggtgc
      421 cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa tgatgatgaa
      481 aaaatttgct ttactggcgg gtctgtttgt tttcgcacca atgacctggg cgcaggacta
      541 caatattaag aacggtttgc catcagaaac ctatattact tgtgcagaag ccaacgaaat
      601 ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta atgccagcgt
      661 tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag tggtagaaaa
      721 actgaatcag gtttgcgcta aagatccgca aatgctgttg atcacggcaa ttgatgacac
      781 catgcgtgct atcggcaaaa aataatattt catatgttac cgcgtcgatg aattcgacgc
      841 ggttttttag ctaatggcgg gatcagaact tatatttcag gcccaccatc attgcggtgt
      901 cattgtagcc attatcaccc agctgcacgc ccacattacc ccagacgctg gccgcaggat
      961 tcagattgcc atttacgcca tgtttcattt cccccagatt acgcgccccg gcttgttcaa
     1021 ctttcacacc gtttattgag acagcaaaat ctttactgtt atggatcccg ttcatttcaa
     1081 caaaaggttc aaagtcgtca ccgtgcggac cgctgttttt ctcctgagta cgaataaagg
     1141 tgtggaaacc aagtcgggtc tggatattgt tatttgcgtc attctcaata cgtgagccat
     1201 tggcttccgt gtgcacatta tgatcgacgc ccatccagat ggcctgtgcc gaggtcgttc
     1261 tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt agccaaaatc
     1321 ggcttgaacg ctcctgttca gttacaggat cgatatagcg cgtttcacct gcacgatcgt
     1381 tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa ctgccagctt
     1441 cgggtcgcag gacgggggta ggcgtttctg ttgattctga agtgacattg
//
LOCUS       DADSZD010000347         1446 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.349, whole genome shotgun sequence.
ACCESSION   DADSZD010000347 DADSZD010000000
VERSION     DADSZD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.349"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            52..>1446
                     /gene="gndA"
                     /locus_tag="J1Z28_004528"
     CDS             52..>1446
                     /gene="gndA"
                     /locus_tag="J1Z28_004528"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBA7719268.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPCKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTE"
BASE COUNT          364 a          356 c          364 g          362 t
ORIGIN      
        1 acttttcatt attgtccgtt tttaaccatc gccccctgac aggagtaaac aatgtcaaag
       61 caacagatcg gcgtagtcgg tatggcagtg atggggcgca accttgcgct caacatcgaa
      121 agccgtggtt ataccgtctc tattttcaac cgttcccgtg aaaagacgga agaagtgatt
      181 gccgaaaatc catgcaagaa actggttcct tactatacgg tgaaagagtt cgttgaatct
      241 ctggaaacgc ctcgtcgcat cctgttaatg gtgaaagcag gtgcaggcac ggatgcagct
      301 attgattccc tcaagccata cctcgataaa ggtgacatca tcattgatgg tggtaacacc
      361 ttcttccagg acaccattcg tcgtaatcgt gagctttctg ctgaaggctt taacttcatt
      421 ggtaccggtg tctccggtgg tgaagaaggc gcgctgaaag gtccttccat tatgcctggt
      481 gggcagaaag aagcctatga actggttgca ccgatcctga ccaaaatcgc cgcagtggct
      541 gaagacgggg agccatgcgt tacctatatt ggtgccgatg gcgcaggtca ctatgttaag
      601 atggttcaca acggtattga atacggcgat atgcaactga ttgctgaagc ctattctctc
      661 ctgaaaggcg gcctgaatct ctctaacgaa gaactggcac agacctttac cgagtggaat
      721 aacggtgaac tgagcagcta cctgatcgac atcaccaaag atatcttcac taaaaaagat
      781 gaagacggta actacctggt tgatgtgatt ctggatgaag cggctaacaa aggtaccggt
      841 aaatggacca gccagagcgc gctggatctc ggagaaccgc tgtcgctgat taccgagtct
      901 gtttttgcac gttatatctc ttctctgaaa gagcagcgtg ttgccgcatc taaagttctc
      961 tctggcccgc aagcgcagcc tgctggcgac aaaggtgaat tcatcgaaaa agttcgccgt
     1021 gctctgtatc tgggtaaaat cgtttcttac gctcagggtt tctctcagct gcgcgcagcg
     1081 tctgaagagt acaactggga tctgaactac ggcgaaatcg cgaagatttt ccgtgctggc
     1141 tgcatcatcc gtgcgcagtt cttgcagaaa ataaccgatg cttatgccga aaatccacag
     1201 atcgctaacc tgctgctggc cccgtacttc aagcaaattg ccgatgacta ccagcaggcg
     1261 ctgcgtgatg tcgttgctta tgcagtacag aacggtatcc cggttccgac cttcgccgct
     1321 gcggttgcct attacgatag ctaccgtgcc gctgttctgc ctgcgaacct gatccaggca
     1381 cagcgtgact atttcggtgc acatacttat aagcgcattg ataaagaagg tgtgttccat
     1441 actgaa
//
LOCUS       DADSZD010000348         1442 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.350, whole genome shotgun sequence.
ACCESSION   DADSZD010000348 DADSZD010000000
VERSION     DADSZD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.350"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            132..536
                     /gene="stpA"
                     /locus_tag="J1Z28_004529"
     CDS             132..536
                     /gene="stpA"
                     /locus_tag="J1Z28_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBA7719269.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(583..1107)
                     /gene="ygaP"
                     /locus_tag="J1Z28_004530"
     CDS             complement(583..1107)
                     /gene="ygaP"
                     /locus_tag="J1Z28_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBA7719270.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVILGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1117..1416)
                     /locus_tag="J1Z28_004531"
     CDS             complement(1117..1416)
                     /locus_tag="J1Z28_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7719271.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          396 a          336 c          339 g          371 t
ORIGIN      
        1 cagaaaataa cagctgaaat aatctcgtgc aggactgtaa atagattaaa ttttgtggaa
       61 atataataag tgatcgctta cactacgcga cgaaatactt ttttgttttg ggttaaaagg
      121 ttttctttat tatgtccgta atgttacaaa gtttaaataa cattcgcacc ctccgtgcga
      181 tggctcgcga attctccatt gacgttcttg aagaaatgct cgaaaaattc agggttgtca
      241 ctaaagaaag acgtgaagaa gaagaacaac agcagcgtga actggcagag cgccaggaaa
      301 aaattagcac ctggctggag ctgatgaaag ctgacggaat taacccggaa gagttattgg
      361 gtaatagctc tgctgcggca ccacgcgctg gtaaaaaacg ccagccgcgt ccggcgaaat
      421 ataaattcac cgatgttaac ggtgaaacta aaacctggac cggtcagggc cgtacaccga
      481 agccaatcgc tcaggcgctg gcagaaggta aatctctcga cgatttcctg atctaattac
      541 tgaggccgga tgtcgctgct atggcgcgtc cggcgtcgct tctcaagctc gttggttcca
      601 cggcatctta tctaacaacc ttgccatccc acaaaaaccg ctaattcctg caaacagtaa
      661 cccggcacca acaaagccgc ttaataagaa gaaaccacta tttacggtat aacccagtat
      721 aacgccgatt aatattaaac cacccgcagc gatctgcacc tggcgcatta acggcaaggg
      781 ttgagattta tttaccgcta ctggtaatcc tgcttttttc cagccatcaa tcccatcttc
      841 gagtaaaaag atttctgcgg gggcggcaat cgctgctaat ttatcggcgt tattactggt
      901 gcgtttacct gcctggcagt ggaatataat ttgctcgcga cgtaatttag ccggaagacc
      961 tgactgttcc agcacggata atggagccag atctgcttca ggaatatgtt cacgaagata
     1021 ttcatcagcg tcacgaatat cgattaactt tgcgccgcgt gcgattaatt cttgtgcatc
     1081 atgcggcgaa atggttgtca aagccatagt gactccttac ggacaataga catttttcag
     1141 ggtggcgata atggcattta ccgcctcatt tttaatggaa tatagaatgc gttgggcatc
     1201 ccgttggctg tcgataagcc cttcgtcccg catacgagcg agatgctgtg atgtcgcaga
     1261 ggcactcagt ccggtaatgc gagtcagctc tcccgcgctg gtgccggggg aaccgctaag
     1321 catgcacaga atcagcaacc gtttagggtg gctcattgct ttcaataagg ccgctgcctg
     1381 ttcggcactg gcctgtaatt gcgcgagttc agtcatagtt atttaagtat tttctaaatt
     1441 aa
//
LOCUS       DADSZD010000349         1431 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.351, whole genome shotgun sequence.
ACCESSION   DADSZD010000349 DADSZD010000000
VERSION     DADSZD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.351"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>1431
                     /locus_tag="J1Z28_004532"
     rRNA            <1..>1431
                     /locus_tag="J1Z28_004532"
                     /product="16S ribosomal RNA"
BASE COUNT          358 a          329 c          454 g          290 t
ORIGIN      
        1 gggtgagtaa tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct
       61 aataccgcat aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg
      121 tgcccagatg ggattagcta gtaggtgggg taaaggctca cctaggcgac gatccctagc
      181 tggtctgaga ggatgaccag ccacactgga actgagacac ggtccagact cctacgggag
      241 gcagcagtgg ggaatattgc acaatgggcg caagcctgat gcagccatgc cgcgtgtatg
      301 aagaaggcct tcgggttgta aagtactttc agcggggagg aagggagtaa agttaatacc
      361 tttgctcatt gacgttaccc gcagaagaag caccggctaa ctccgtgcca gcagccgcgg
      421 taatacggag ggtgcaagcg ttaatcggaa ttactgggcg taaagcgcac gcaggcggtt
      481 tgttaagtca gatgtgaaat ccccgggctc aacctgggaa ctgcatctga tactggcaag
      541 cttgagtctc gtagaggggg gtagaattcc aggtgtagcg gtgaaatgcg tagagatctg
      601 gaggaatacc ggtggcgaag gcggccccct ggacgaagac tgacgctcag gtgcgaaagc
      661 gtggggagca aacaggatta gataccctgg tagtccacgc cgtaaacgat gtcgacttgg
      721 aggttgtgcc cttgaggcgt ggcttccgga gctaacgcgt taagtcgacc gcctggggag
      781 tacggccgca aggttaaaac tcaaatgaat tgacgggggc ccgcacaagc ggtggagcat
      841 gtggtttaat tcgatgcaac gcgaagaacc ttacctggtc ttgacatcca cggaagtttt
      901 cagagatgag aatgtgcctt cgggaaccgt gagacaggtg ctgcatggct gtcgtcagct
      961 cgtgttgtga aatgttgggt taagtcccgc aacgagcgca acccttatcc tttgttgcca
     1021 gcggtccggc cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg
     1081 acgtcaagtc atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca
     1141 aagagaagcg acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg
     1201 agtctgcaac tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg
     1261 gtgaatacgt tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa
     1321 agaagtaggt agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt
     1381 gaagtcgtaa caaggtaacc gtaggggaac ctgcggttgg atcacctcct t
//
LOCUS       DADSZD010000350         1427 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.352, whole genome shotgun sequence.
ACCESSION   DADSZD010000350 DADSZD010000000
VERSION     DADSZD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.352"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(185..1285)
                     /locus_tag="J1Z28_004533"
     CDS             complement(185..1285)
                     /locus_tag="J1Z28_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hsdR"
                     /protein_id="HBA7719272.1"
                     /translation="MFDVDDTAPDTPAPRELSQDVQALINNGLDFLDKAREELEASKP
                     KFSVVSFWTAVEILLKVPLAHEHWSLVCSPKKPIKKQAYLAGDFQSVTYEETRERLKD
                     VLERPLDRETDSAFDKVRKHRNRVVHFYHPTFTEAEQRQILKEQADAWFALNRLLRDE
                     WKVIFGVKHNWKLAFGETRLIRGNKFYAEVRFKQVKPELEQLSEKNIQIGNCNECHQH
                     ATVTGTETTGNENRKLEVTRCKVCTSAVRQITLVCPDCEIPQLLPEGDSDFECEHCDY
                     TSDRYKLLDEELFHSVDEQLLSVFPAGCTNCMTPESVCKFGDGYLCTQCFIYYTELHV
                     CGCCGHLSDSVPELSHIRGCEFCDGDQRYFDD"
BASE COUNT          367 a          351 c          318 g          391 t
ORIGIN      
        1 caccggaaag gtggcggggg attcacaacg ataatatctg tctgaaatta tttggaatgc
       61 aggtatatgt ggctgatttg tatcagctaa tgagtacagg tctgaatcta atctgacagt
      121 ccgctctgtg ccaggagcgg actgtccaac ccatccctga agagcctaga ttatgtatgg
      181 taattcagtc atcaaaataa cgttggtcac catcacaaaa ttcacatcct cggatatggc
      241 tcagttcagg aacgctatcg ctcaggtgac cgcaacaacc acatacatga agctcggtgt
      301 aatagatgaa acactgagtg caaagatagc cgtctccaaa tttacatacg ctttcaggag
      361 tcatgcaatt ggtacatcca gcaggaaata cggagagtag ctgttcatcc acactgtgga
      421 acaactcctc atccagcagt ttatacctat ccgaagtgta gtcgcagtgt tcgcattcaa
      481 aatcactatc tccctcaggt agcaattgag gtatttcgca gtctggacaa actagggtaa
      541 tttgccttac agcactggtg caaaccttac accgcgtaac ttcaagctta cggttttcat
      601 taccggtcgt ttcagtccct gttaccgtcg catgctgatg gcactcattg cagttaccga
      661 tttgaatatt cttttcagag agctgctcga gttcaggctt aacctgttta aaccgcacct
      721 cggcataaaa tttgtttccc ctaatcagac gagtttcacc gaaagccagc ttccaattat
      781 gtttgacacc gaagataact ttccattcat cccttaataa ccggttcagg gcaaaccagg
      841 cgtcagcttg ttctttgagt atttgacgtt gttcagcctc ggtgaaagtg gggtggtaga
      901 aatgaactac gcggttgcgg tgctttctga ccttatcaaa agcactgtcc gtttctctgt
      961 ctaggggtct ttcaagtacg tccttaagcc tttcacgggt ttcttcgtag gtcaccgact
     1021 gaaaatcacc ggctaggtaa gcctgctttt ttattggctt tttcgggctg cagaccaacg
     1081 accagtgttc gtgcgccagc ggaactttca gtaaaatctc gaccgcagtc cagaaactga
     1141 cgacggagaa cttcggcttc gaggcctcca actcttcacg ggctttatcc agaaaatcca
     1201 gaccattgtt gatcagcgcc tgcacatcct gactgagttc acggggggcg ggagtatcgg
     1261 gtgcagtatc gtcaacatcg aacatcatta tctcctttaa tcagcttcca ccacgcagtt
     1321 gctacagcgc gttagttgta tccagtgtaa actggacttg gtagctggca gccacatcga
     1381 gcgcaaaaaa ccgtaaatta acttctgtag actcgtactt ttcgtga
//
LOCUS       DADSZD010000351         1417 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.353, whole genome shotgun sequence.
ACCESSION   DADSZD010000351 DADSZD010000000
VERSION     DADSZD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.353"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            165..452
                     /locus_tag="J1Z28_004534"
     CDS             165..452
                     /locus_tag="J1Z28_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719273.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSQGVDKQFRHINIAEADHFKQFLCQIKRAGLNIRAIC"
     gene            574..1395
                     /locus_tag="J1Z28_004535"
     CDS             574..1395
                     /locus_tag="J1Z28_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA7719274.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTDNMEAMKEIHLNSDEQHLFGRAALMVRYEDENKTPVTPEQIITPRRREDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWVIAEQFRKWKS"
BASE COUNT          413 a          284 c          374 g          346 t
ORIGIN      
        1 tgcgtcaggc cgtggcagtg atgccccttg cggcgaaggt tctcaaacag gtgatggtta
       61 ataccggagg gaacaaaagg caggtccccg caaaacgtca aaaccgcggc tcccgaaact
      121 caacagatga tgggggctga aaaccagata gaggagaaag caggatgagc acccgaaata
      181 tccacgttaa caccgcgtcg tacacgcttc ttgttgccgg gaagaaaaaa aacaccgggg
      241 aagagtggga cgtcctggaa ttcagcagcc tgactgagct gaaaaaatat cgcaaaagcc
      301 acccggaaaa gatggccttc agttatagct acgcgctcag tcagggtgtg gataagcagt
      361 tccgccatat caacattgcg gaagccgatc acttcaaaca gttcctgtgt cagattaagc
      421 gtgccggcct caatatccgg gcgatctgct gaccggatgt gatgaatacc tctcctgatt
      481 gttgcaatct tgttaatgga gatcgggggg ggggatgata ttaagcggaa aaatttttta
      541 ttaaatttat gctttcataa aggagtagct gttatgcgat tagcttcccg ttttggtcgg
      601 tataattcca tccgccgtga acgtccttta acggatgatg aattaatgca gttcgtgcct
      661 tcggtatttt ccggtgataa acatgagtcc cggagtgaac gttatacgta tattccaaca
      721 atcaatatca tcaataagtt acgtgatgaa ggtttccagc cattctttgc ctgtcagagt
      781 cgggttcgtg atttggggcg tcgagaatac agtaaacata tgttacgtct tcgcagggaa
      841 gggcatatta acgggcagga agttcctgaa attatcctgc ttaattcaca tgatggttca
      901 tccagttatc agatgatccc cggaattttt cgttttgtct gcacaaatgg cctggtgtgc
      961 ggaaataatt ttggcgaaat ccgcgttcca cataaaggtg atattgtcgg gcaggttatc
     1021 gagggagcgt atgaagtgct cggtgtcttt gataaggtca ctgataatat ggaggcgatg
     1081 aaagaaattc atcttaacag tgacgagcaa catttatttg gcagagctgc actgatggtc
     1141 aggtatgaag atgaaaataa aacgccagtg acacctgaac agataattac tccccgtcgt
     1201 cgggaagata aacagaacga tctctggacg acctggcagc gggttcagga gaatatgata
     1261 aaaggcggat tatcggggcg aagcgcctcc gggaaaaata ccaggacaag agccattaca
     1321 ggtattgatg gtgatataag aatcaacaag gcgttatggg tgattgccga acagttcaga
     1381 aagtggaagt catgataata taagtaaagc cccgaaa
//
LOCUS       DADSZD010000352         1414 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.354, whole genome shotgun sequence.
ACCESSION   DADSZD010000352 DADSZD010000000
VERSION     DADSZD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.354"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(69..878)
                     /locus_tag="J1Z28_004536"
     CDS             complement(69..878)
                     /locus_tag="J1Z28_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719275.1"
                     /translation="MSLPVPFGAIVTVVKKCWSTLTQIMLIKTYQLSHERWAVRHRLG
                     ASWHSLGNYLEYSLRFAQLTDKEPRCSRIAFRSKEERLQQVELVFEAIGSGLRYQETL
                     CIHDVNNSPIILTLSNIPTLDVLILDDGFAFTVEQYQLKYCTIKLMTGESISIDNSPK
                     YSLMQNWCLNDTWKRRWGIIWNCNAIECARRRIAEYWKWGFCLPLVGRPPLYSPSRKG
                     IHLFEAKPLRWFMTRPWCLNIQFWTAIWSGLFILSDENVLQWRWKEPPQQD"
BASE COUNT          404 a          323 c          278 g          409 t
ORIGIN      
        1 attagacaag ttaatctaac cagcactgag gtccgctcct cgctcagagc ggacggcctt
       61 cgtgaaagtt agtcttgttg tggtggctct ttccagcgcc attgcaagac attctcatcg
      121 ctgagaataa acagtcctga ccaaattgcc gtccaaaact ggatattcag acaccatggg
      181 cgtgtcataa accagcgtaa cggttttgct tcaaacagat gaataccttt tcgcgatggt
      241 gaatataacg gggggcgtcc cactaacggc agacaaaagc cccatttcca atattcagca
      301 atacgccttc gggcacattc aattgcatta cagttccaga tgatacccca acgccgtttc
      361 catgtatcat tgagacacca gttttgcatt agcgaatatt tgggcgaatt gtcaatagat
      421 atactttcac cagtcattaa ttttatcgtg caatatttta actggtactg ttcaaccgta
      481 aatgcgaatc catcatctag aataagtaca tcaagtgtag gaatatttga aagcgttaat
      541 attatcggtg agttatttac atcatgaatg catagtgttt cttggtagcg taagccactg
      601 ccaatggctt caaaaacaag ttcaacttgt tgcagtcgtt cctctttgct tctgaaagca
      661 atacgtgaac aacgaggttc tttatctgtc aactgagcaa atcgtagact gtactccaaa
      721 taattcccta aggaatgcca gctcgcccct agcctgtgtc tcactgccca gcgctcatgt
      781 gagagttgat aagtttttat cagcattatc tgggtaagag tagaccagca tttcttcact
      841 actgtaacaa ttgcaccaaa aggaacagga agtgacatta ctaaacccct acaaatatca
      901 gataattagg ttttatatca tttcagtcag tgtaatcaaa tgataatcag gattggggct
      961 aatcttgaac gccactaaag atcggcaaca atggggatcc aatgtttcgt tagtgatttt
     1021 tcgcattagc agatacatgc ttacttccgc tcctcgctct cagcggacct tcagctcagt
     1081 gatatcgtcc gctctgtgcc aaaggcggac gctcaagtag atactgtcct gacagtatgc
     1141 ggtaatcaga gttgttatat attacttgcg tccaaaccag ctttctgaca acccggccgc
     1201 tcctgctttc aacaacgcgc cgccatactt ttatgctgca aagagcggag acagaaggtt
     1261 agcgtaattt gtaaataact tacattcacg tgtgatctgg atcataattt tgcctatgca
     1321 ccgatcgtaa tataaatatt tacataacga tcagtgattc atgaaaactc aaataacttt
     1381 cgctgcgctt ttgccagcat tagcgtcttt cata
//
LOCUS       DADSZD010000353         1345 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.355, whole genome shotgun sequence.
ACCESSION   DADSZD010000353 DADSZD010000000
VERSION     DADSZD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.355"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(48..1229)
                     /locus_tag="J1Z28_004537"
     CDS             complement(48..1229)
                     /locus_tag="J1Z28_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719276.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAIKRHKQQLGAIEEYTQWL
                     KKEPRASYFFLFRLYTRIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSAVVPPTNENTSHPEDTPDNTPAGGNTGQAEKTRNSGLTPIPEKRSGM
                     PPEHLRFATGFPPQPKIAGPKGKPMRTVHPDKIYREIIWFCSGYLRKSGPEATRTIIN
                     SIFCEWASINNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLESNPRRNRAKFAINSFQTLGVKQHKAVLLDELKSAREQQKR
                     RDERADGSVPLKLSGKIHKQLESVARSRGVPPKKLLNEMIEQAYRDLVATRQNSQIDS
                     R"
BASE COUNT          299 a          321 c          356 g          369 t
ORIGIN      
        1 caccggaaag gtggcggggg attcacggag ataatatatg tctgaaatta tctggaatcg
       61 atctggctgt tctgcctggt tgccaccaaa tcccggtacg cctgctcaat catttcattc
      121 agcagttttt tgggggggac accacgactt cgggcaacac tttcaagctg tttgtggatt
      181 tttccggaca gtttcagcgg gacggaacca tcagcacgtt catcacgcct tttttgttgc
      241 tcccgggcac ttttcagttc atcaagaaga actgctttat gctgtttaac cccgagagtc
      301 tggaaggagt ttatcgcaaa cttcgctctg tttcgtctgg gattactttc cagtaaaaac
      361 tgtacctgtt gctcattcca gccttcccag ttatcaaatg tggcgcaggc gaaccagtat
      421 ttctgctccg gggtaagggg attcagcggg gttcccacac accggacctg catggcgttc
      481 cataaccagt cacactgttc actgtccctg ctgtccaccc acgaaaaggg ggaggggtaa
      541 tcattgttta tgctggccca ttcacagaat attgagttga tgatcgtcct cgtagcctct
      601 ggtccggatt tccgcaggta accggagcag aaccatataa tttcacggta gattttatcc
      661 ggatgcacgg tgcgcatcgg ctttcctttg ggtccggcaa tcttcggttg cggtggaaac
      721 cctgtggcaa accgcagatg ttccgggggc attcctgaac gtttttcagg aatgggtgtg
      781 agtcctgagt ttctggtctt ttccgcttgt ccggtattgc ctccggccgg agtgttgtcg
      841 ggagtatctt ccggatgcga ggtgttttca ttagttgggg gaacaacagc agaactgtcc
      901 atctccggaa tgggttgagg ggcataatca ctgtggtagc ccggggaaag cgttaagtcc
      961 cgaagggtga catcaggttc cgggcagtga gtgccaccgg gagtgaacgg gagttgttgc
     1021 ttttggggga aaaatgtatt gtgaatgcgg gtatacagcc ggaacagaaa aaaataagat
     1081 gcccgtggtt ctttttttaa ccactgtgta tattcctcta tggcaccgag ctgttgtttg
     1141 tgccgtttta tagcatcgat atacggacag ctggtgcaga tatcatccag ttcctttccc
     1201 acaatacacc tcacctgctc ttcccacatt tgcagatttt aagaaagcaa acaaaaacct
     1261 ccgtacatga tgtacagaat gtacagaaca atctggcagg tactggcgtc agaagagctc
     1321 cgggccgcct gcactttccg gaatg
//
LOCUS       DADSZD010000354         1313 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.356, whole genome shotgun sequence.
ACCESSION   DADSZD010000354 DADSZD010000000
VERSION     DADSZD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.356"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>1313)
                     /locus_tag="J1Z28_004538"
     CDS             complement(<1..>1313)
                     /locus_tag="J1Z28_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBA7719277.1"
                     /translation="TALVGVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSG
                     IWDGTFKPAYSNNMAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGF
                     GGTEPRITCNAWLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRRSDKVWTYNRS
                     NVVMPDDGAPFRYSFSALKDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTK
                     MDAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIS
                     TGGRVLAVNSQTRTLTLDREITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSR
                     VPDGVAGYSVWGLKLPTLRQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFD
                     GDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVS"
BASE COUNT          249 a          457 c          339 g          268 t
ORIGIN      
        1 aagctcaccc ccttcaccac cttcggcgta tcccagcgcg ccagcacctg gtattccccg
       61 ctgtctgcgg tgacttctgc ggtcagatgc tgcactgctg gcggcgtgac accattcacc
      121 gtgccgctct ggtcaccgtc aaagtgcgcc ccgttatcca cgatggcttc tttctccggt
      181 acatgctgca cggcggtgat ggcatacgtg ccgtcatcgt tctcacggat actcacgcag
      241 cggaacaggc gctggcgcag cgtcggcaac ttcagccccc acacgctgta tccggcaacg
      301 ccgtcaggaa cacggctcac ttttaccttc acaccgtcgg tgacggactg gacctccacg
      361 ctgaccggat tgccacttcc gtcaaccagg cttatcagcg tggtaccgga ggatggcagc
      421 gtgatttcac ggtcgagcgt cagcgtccgg gtctggctgt tcaccgccag cacgcgccca
      481 ccggtgctga tccccgcata gtcatcatcg cagatttcaa taacatcacc cggcacatgg
      541 cgaagccctt cggcacccac gctgaaatcc acggtctgcg tctccagcag ctccgtttta
      601 atcagccaca acccggcgcg gtgtgcctgc ccccggctgg tacagccaaa ggcatccatc
      661 ttcgtgacgt tacgaccgta acggagaatg gcctgcgtgt cctccacaag ctctgtcgcc
      721 gtctcccagc cgttatccgg gtcaatccag ttcacctcaa cggcattatg gcggtccttc
      781 agggcgctga agctgtagcg gaacggcgcg ccatcatccg gcatcaccac attactgcgg
      841 ttataggtcc acaccttatc cgaccgtcgg tcctgcacga acgtcagcgt ctgcccgttc
      901 cataccggca tacagcgcat cgccgagcag aaatcactga gaacatccca cgccttacgc
      961 tgtgtggtca gccaggcatt acaggtgatg cgcggctccg tgccgccaaa accgtccggc
     1021 accgactggt cgcaatactg gccgatgaca tacagcgccc atttgtccac atccgccgca
     1081 ccaagacgtt tccccatgcc gtagcgcgga tgggtcagca tatcccacag acaccaggcc
     1141 atgttgttgc tgtatgccgg cttaaacgtt ccgtcccaga taccgctgta ttgccgcgtc
     1201 tgcgggttat agttcgacgg cacctgcaga atgcgcccgc gcagatgata attacggctc
     1261 acctgctggc tgccgaactg ctccgagtcc acctgtacgc cgaccagtgc cgt
//
LOCUS       DADSZD010000355         1290 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.357, whole genome shotgun sequence.
ACCESSION   DADSZD010000355 DADSZD010000000
VERSION     DADSZD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.357"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..719
                     /gene="sdiA"
                     /locus_tag="J1Z28_004539"
     CDS             <1..719
                     /gene="sdiA"
                     /locus_tag="J1Z28_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBA7719278.1"
                     /translation="DTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSLCV
                     RHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSEAQ
                     PLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRESL
                     MALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNMQK
                     KINAPNKTQVACYAAATGLI"
     gene            complement(787..1011)
                     /locus_tag="J1Z28_004540"
     CDS             complement(787..1011)
                     /locus_tag="J1Z28_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBA7719279.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(998..1174)
                     /locus_tag="J1Z28_004541"
     CDS             complement(998..1174)
                     /locus_tag="J1Z28_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719280.1"
                     /translation="MIKIFIGHYINVFYSTVDITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
BASE COUNT          340 a          266 c          298 g          386 t
ORIGIN      
        1 aggatacgga ttttttcagc tggcttcgca cgatgttgtt gcgttttcag aggatggagg
       61 ccgcagaaga ggtctaccat gaaatagagc ttcaggctca gcagctggag tacgattact
      121 attcgttatg tgttcgccac ccggtaccat tcacgcgacc taaagtggct ttctatacca
      181 attaccctga gtcgtgggtt agttattatc aggcaaaaaa ttttctcgca attgatccgg
      241 tgctgaaccc tgaaaacttt agtcagggcc atttaatgtg gaatgatgac ttattcagcg
      301 aagcacagcc gttatgggaa gctgcgcgcg cacatggttt acgccgcggt gtcactcagt
      361 atttaatgct gccaaatcgg gcgttgggct ttttgtcctt ttcccgttgc agcacgcgcg
      421 aaatacccat tcttagtgat gaactgcaat taaaaatgca gctactggtg cgcgaaagtc
      481 tgatggctct gatgcgttta aatgatgaaa tagtgatgac gccagagatg aatttcagca
      541 agcgcgaaaa agaaattctg aaatggacgg cggaaggtaa gacatcagca gagatagcga
      601 tgattttgtc aatctctgag aatacggtca atttccatca gaaaaacatg cagaaaaaaa
      661 tcaatgcacc gaataagacg caggttgcct gttacgcggc cgctactggc ttaatttgat
      721 ctcttttctg ttctgcgtac cagatgcaaa aaccggctga aaggcacgct ctcagccggt
      781 tttatattac tgacggtagg cttgtttaat ttgcttaacc gttttggaaa atactgcagc
      841 ctgggtttcg tcttcgatca gcgcaagctg tttttccatc tttaacatca cgcggcccgc
      901 gtcagcttgt cccatcgcct gcagcatcag cgtcagcatc gctttcaggc aggagacttc
      961 atttgccagt tcttgattat tctcggcagt agaaaaatca ggcgtactca tttattttcc
     1021 tcgtcatgtt gcaatgaaaa tttgcggtga aaaatgttaa ggcggcggag tataccataa
     1081 gctttgctaa aaatagcagt ggttgttttt tgagcgtgat atcaacagta ctataaaata
     1141 cgttaatata atgaccaata aatattttta tcatgaatgt tttttgcccg tattgttcgg
     1201 gttaattaat gttacatatt cagcgggctg attttcattt ttgctgaata aagtcaattt
     1261 ttgtcacatt tcatcgccgg gcttactgtg
//
LOCUS       DADSZD010000356         1271 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.358, whole genome shotgun sequence.
ACCESSION   DADSZD010000356 DADSZD010000000
VERSION     DADSZD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.358"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(21..653)
                     /locus_tag="J1Z28_004542"
     CDS             complement(21..653)
                     /locus_tag="J1Z28_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBA7719281.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRISGRDVSTSGLTAQLHEVLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(590..>1271)
                     /locus_tag="J1Z28_004543"
     CDS             complement(590..>1271)
                     /locus_tag="J1Z28_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7719282.1"
                     /translation="GFVVSTPEGERYFPCVNISGEPEDYFRMAPEDWLQAEMQGEIVA
                     LVHSHPCGLPWLSEADRRLQVQSDLPWWLVCRGAIHKFRCVPHLTGRRFEHGVTDCYT
                     LFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEATGLYQVPLSSAQPGDVLLCCFG
                     SSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFTGIYND
                     LAAASTFV"
BASE COUNT          298 a          422 c          315 g          236 t
ORIGIN      
        1 ttcacataaa acattttgca tcagcgacca atcaccacaa cctgaccacc atccccttcg
       61 tctgccgtgc tgatctcctg agaaaccacg cgtgacccca cgcgcatttc accgtacaga
      121 acaggcagaa cattgccctg ggcaaccatg ttatccagtg acgagaaata cgtgttctgc
      181 ttaccgttat ccgttgtctg tgtacgggga gttctggctt tcggtgccag catctgcgcc
      241 acaccaccaa gtaccatact ggcaccgaga gaaaacagga taccggtcat accaccggcc
      301 ccaatggctg ccccccatgc tgcaagggtg gctccggcag taaagaatga tccggcaatg
      361 gcggcagccc ccagaacaat ctggaatacg ccccctgact tggccccggc gactctggga
      421 acaatatgaa tcacagcgcc gtcaggcaga acctcatgta actgcgccgt caatccggac
      481 gtgctgacgt cccgcccgga aatccgtacc tgataccagc cgtcgctcag tttctgacga
      541 aacgccggga gctgtgtggc cagcgcccga atggcttcag cccccgtttt cacacgaagg
      601 tcgatgcggc ggccaaatcg ttgtaaatcc ccgtaaaggc agatgcgcgc catgcccggt
      661 gacgccagag ggagtgtgtg cgtcgctgcc atttgtcggt atacctctct cgtttgctca
      721 gttgttcagg aatatggtgc agcagctcgc cgtcaccaca gtaaatggcg gcatgattcg
      781 gcaccgatga accaaaacag cacagcagca catcgcccgg ttgtgctgat gacaacggca
      841 cctgatacag ccctgtggcc tccagattat ccagatagag attctgaccg tgacgccacc
      901 agtcatcccc gcgatgaaaa tccggcatct caatccccgc cagatgataa gcatcccgga
      961 acagcgtgta acagtccgtc accccgtgct caaagcgccg cccggtgaga tgcggcacac
     1021 agcggaactt gtgaatcgcc ccccggcaga ccagccacca cggcaaatca ctctgcacct
     1081 gcagccgccg gtcagcctca ctcagccagg gcagaccaca gggatgactg tggaccagtg
     1141 ccacaatctc accctgcatc tctgcctgca gccagtcctc cggagccatc cggaaataat
     1201 cctccggctc accggaaata ttcacgcagg gaaaatatcg ttccccctcc ggcgtgctta
     1261 ccacgaagcc g
//
LOCUS       DADSZD010000357         1243 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.359, whole genome shotgun sequence.
ACCESSION   DADSZD010000357 DADSZD010000000
VERSION     DADSZD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.359"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            82..>1243
                     /locus_tag="J1Z28_004544"
     CDS             82..>1243
                     /locus_tag="J1Z28_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7719283.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLVLMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRAFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSSSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPF"
BASE COUNT          341 a          269 c          281 g          352 t
ORIGIN      
        1 gcctgattca gtgctacgca ttgaatcagg ttcttttcgc gttgaacaat atcaagatac
       61 ggctattcgc agggagtgga tatgtttacc aacgtaaatg ttgattgttg caaaacacca
      121 ggatgtaaaa acctggggtt gctgaatagc caggattatg tcgcacaggg taaaaatatt
      181 ttatgccgtg aatgtggtta cttgtttcca gtgatatctg aacagtcgct taatatttat
      241 cgtaatattg tgaatcactc ctggagaggt ttgatttgcc aatgttcaac ttgcggaggc
      301 acgtccctca aaaaatatgg atattctgca caaggccaga gaagaatgta ttgccatcat
      361 tgtgagaaaa catttatcac tctggaacat gtaattacca caccacgagg agcccagtta
      421 gtattgatga ttgagcaagg ggaggcactt gcggatatca gaaagtcatt acttcttaac
      481 agcactggac ttagccgtga actgttaaaa ttagcgcgtg aagcaaacta taaagaaagt
      541 cgacagtgtt tccctgcttc tgatattacc ctgagtaccc gcgcttttcg cgtcaagtat
      601 aatggtagca ataactctct ttatgctctt gttaccgcag aagaacaaag cggcagggtg
      661 gttgccatct caaccaatta ctcctcatct gccgtagagc aacattatca atacacatcg
      721 aactatgaag agcgtatgtc tccagggacg ctggcacatc atgtccagcg caaagagtta
      781 cttactatgc ggcgggatac cttgtttgat attgattacg gcccggcagt tttacatcaa
      841 aacgatccgg gaatgttggt aaaaccggtt cttccggcat atcgtcattt tgaactggtc
      901 agaatactga ccgatgagca ttccaacaac gttcagcatt accttgatca cgaatgcttt
      961 atattgggcg gctgcctgat ggctaatttg cagcatattc atcaaggtcg ctgccatatt
     1021 tcctttgtca aagagcgcgg tgtggcaccc gccaccattg attttccacc gcgattattc
     1081 cttagtggtg gggtacgaaa taatgtctgg cgtgcatttt ctaaccgcaa ttattcaatg
     1141 gctgtatgca atctcactgg cagtaagaaa gtccgcgaga tgcggcatgc aacattgaac
     1201 agtgcgacgc gttttatcca ctttgtggag aaccatcctt tcc
//
LOCUS       DADSZD010000358         1227 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.360, whole genome shotgun sequence.
ACCESSION   DADSZD010000358 DADSZD010000000
VERSION     DADSZD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.360"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            109..711
                     /locus_tag="J1Z28_004545"
     CDS             109..711
                     /locus_tag="J1Z28_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBA7719284.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRFLQCGNEIYSAIKDLQRKAPDKNIVIFTHNH
                     CLTYIAKDKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(750..1175)
                     /gene="nudI"
                     /locus_tag="J1Z28_004546"
     CDS             complement(750..1175)
                     /gene="nudI"
                     /locus_tag="J1Z28_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBA7719285.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEPGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     RDVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          339 a          280 c          261 g          347 t
ORIGIN      
        1 ccggcatcat aggattttta atattacacc aactgctaat tatcgccaga aaagtcgctc
       61 aatttcacgg taattgtctg gttgcgcttg tctacaggtg gagtttacgt gttagctttt
      121 tgccgatctt cgttgaagtc aaaaaaatat ttcatcattt tactggcgct cgctgcgatt
      181 gccggactgg gtactcatgc cgcctggagt agcaatggtt tgccacgcat cgacaataaa
      241 acactggcca gactggcaca gcagcacccg gttgtcgttt tgtttcgcca tgctgaacgt
      301 tgcgatcgtt caaccaatca atgcttgtca gataaaacag gtattacggt caaaggtacc
      361 caggatgccc gtgaactggg caacgctttt agtgctgata tccctgattt tgatctctat
      421 tccagtaata ccgtccggac tattcagtcg gcgacctggt tttcggcggg taaaaagctg
      481 acggtagata aacgatttct tcaatgcggt aatgagattt atagtgcaat taaagactta
      541 caacgcaaag cacctgataa aaatatcgtt atttttaccc ataatcattg cctgacatat
      601 attgctaaag ataagcgtga tgcgacattt aaacctgatt atctggatgg tttagtcatg
      661 catgtggaaa aaggcaaagt ttatctggat ggggaattcg ttaaccacta aattttcaat
      721 ctgacagcca gtgatggctg tcatcattgt taaagaagac ctttcaaacg taacgttttt
      781 cgggtggcaa cattcaaatc ataatgcacc agatcttcag gttttaccca cgcgtagtcc
      841 tgaaactctt cgtttatttt cacatctcgg ttggcagaaa cgcagtcaaa aatcaggtaa
      901 atcatataaa tctcttcctt gcgaccatct gcatacgtct tggtgcgaat atcatcgctg
      961 aaggtccacg gcgtgatttc tgtcaaaagc agctgttctc ccagttcttc gcgaatttcg
     1021 cggcgtagtg cctcttcaat tcgttcgcca ggctccacgc cgccacccga aagcgcccat
     1081 tgaccgggga aaacgccgcg atcgtcggcc attttacaca gcaaataagc accatcattt
     1141 tgaatcaaag ggcatacaat agtccgttgt cgcacagggg tattccttat aaataaaaag
     1201 aaaccatgag aatacggtaa ctcagaa
//
LOCUS       DADSZD010000359         1213 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.361, whole genome shotgun sequence.
ACCESSION   DADSZD010000359 DADSZD010000000
VERSION     DADSZD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.361"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>1213
                     /locus_tag="J1Z28_004547"
     rRNA            <1..>1213
                     /locus_tag="J1Z28_004547"
                     /product="23S ribosomal RNA"
BASE COUNT          306 a          271 c          376 g          260 t
ORIGIN      
        1 aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag gtggtcaggt
       61 agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa tggtgccgta
      121 acttcgggag aaggcacgct gatatgtagg tgaagcgact tgctcgtgga gctgaaatca
      181 gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg caaacacgaa
      241 agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat ggggttagcg
      301 gtaacgcgaa gctcttgatc gaagccccgg taaacggcgg ccgtaactat aacggtccta
      361 aggtagcgaa attccttgtc gggtaagttc cgacctgcac gaatggcgta atgatggcca
      421 ggctgtctcc acccgagact cagtgaaatt gaactcgctg tgaagatgca gtgtacccgc
      481 ggcaagacgg aaagaccccg tgaaccttta ctatagcttg acactgaaca ttgagccttg
      541 atgtgtagga taggtgggag gctttgaagt gtggacgcca gtctgcatgg agccgacctt
      601 gaaataccac cctttaatgt ttgatgttct aacgttggcc cgtaatccgg gttgcggaca
      661 gtgtctggtg ggtagtttga ctggggcggt ctcctcctaa agagtaacgg aggagcacga
      721 aggttggcta atcctggtcg gacatcagga ggttagtgca atggcataag ccagcttgac
      781 tgcgagcgtg acggcgcgag caggtgcgaa agcaggtcat agtgatccgg tggttctgaa
      841 tggaagggcc atcgctcaac ggataaaagg tactccgggg ataacaggct gataccgccc
      901 aagagttcat atcgacggcg gtgtttggca cctcgatgtc ggctcatcac atcctggggc
      961 tgaagtaggt cccaagggta tggctgttcg ccatttaaag tggtacgcga gctgggttta
     1021 gaacgtcgtg agacagttcg gtccctatct gccgtgggcg ctggagaact gaggggggct
     1081 gctcctagta cgagaggacc ggagtggacg catcactggt gttcgggttg tcatgccaat
     1141 ggcactgccc ggtagctaaa tgcggaagag ataagtgctg aaagcatcta agcacgaaac
     1201 ttgccccgag atg
//
LOCUS       DADSZD010000360         1181 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.362, whole genome shotgun sequence.
ACCESSION   DADSZD010000360 DADSZD010000000
VERSION     DADSZD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.362"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            134..241
                     /locus_tag="J1Z28_004548"
     CDS             134..241
                     /locus_tag="J1Z28_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719286.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(294..1052)
                     /locus_tag="J1Z28_004549"
     CDS             complement(294..1052)
                     /locus_tag="J1Z28_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBA7719287.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          335 a          287 c          272 g          287 t
ORIGIN      
        1 tttttcataa aatgcagaaa cagtgaccag gtgtacaacg ccacccgacg actgtaataa
       61 caaaaaatcc cttgatgcct gtcccttttg ttacactccg ttatcacgca caagagatat
      121 gcaggacact ggtatgccga ctaaacgctt tgataaaaaa cactggaaga tggtggtggt
      181 gctactggca atctgtggcg ctatgttgtt gctacgttgg gctgcaatga tttggggctg
      241 agcagtgaca aacgaaaaca gccagaccgc ataacacggt ccggcagata attttacatg
      301 ctgtaaccca acgataaggt tgtccgacga tcggtatgtt ccggtgcaga ctctggaggt
      361 tcagagttcc atgtaacgtt gtaagccacc ttcagtccaa aatgttcatt aatagcaaca
      421 tttaatgcgc tttcggagtt cagcgttgtg tcttccgcgc caaagaccga aacaccctgc
      481 gtaaatttag cgttgtcagt caactgccat gcataagccc cggaggcata gcccagcggc
      541 tgggtttcac tggcattgtc ggtatatttg tcgtaacgca caccagggcc gaattcaaag
      601 cggaaactgt gtaccgggcc attcaaaaac tggcgaccat aacccgcggt caacacatcg
      661 cgctctcgat agccgttata acggtctgtc agccagcttg cctgaccaaa taaatagtca
      721 taatcagtta aattaaaacg gctacgcccg cccgccgcat atttttctga agaacgctca
      781 tcattagaag aagtattact ggcgttcccc cacagcgacc aggcagtggt ttgtccatac
      841 caggtcatag tggtgtcagc cgtaagtgag gagcttttcg tattgcctga ttgtgcaaga
      901 tatcctgcgt tcagattacc ttcgaaaggt tttttagcgc tggcaggatc gtccatgaca
      961 gtaaaaacgg aatcatcggc agctgcattc agtgacgcaa acatgccccc cgccaacata
     1021 acgatggcag gaactgtctt caaaagcttc atttatcaag agtccgtaca acaaaaaaag
     1081 agaccatcgc ggtcccggaa actttcttaa ggatcaaaga ttagcgtccc tggaaaggta
     1141 acgaattata aaaaggcgcg aataacttag caatgtattc t
//
LOCUS       DADSZD010000361         1158 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.363, whole genome shotgun sequence.
ACCESSION   DADSZD010000361 DADSZD010000000
VERSION     DADSZD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.363"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            1..1158
                     /locus_tag="J1Z28_004550"
                     /pseudo
     CDS             1..1158
                     /locus_tag="J1Z28_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
BASE COUNT          332 a          269 c          309 g          248 t
ORIGIN      
        1 atttctacgg caaaactctt tcaactttct cacaccagcc tctcacactg gattaatctt
       61 tttcttcttc acggtcctcg ggcactggat tgcagacaca agcgtagcta ttctcctgaa
      121 gataaacttt gcgtggttct ttatgctctc gggcattctg agtctctacc ccgggtagca
      181 gcccggttta acattcccag ccacaacacg gttaaaaact ggataaaagg ctaccgcaaa
      241 tctggtaatg aggcctttat caggcgcagg aaggagaaaa gcatgacccg ttctgatgat
      301 acccatgaaa acgaggcaaa catgacgccg gaagagatga agaatgagct gcgttatctt
      361 cgcgcagaaa atgcatatct gaaagccatg caggagcatc ttctggaaaa aaagcgccag
      421 gagctggaga aaaaacgaaa gtcatccaga gcctgaggta cggacactgc cagtctgacc
      481 tgttaaaggc cgcagggctg gccagaagca cgctgtacta ccagctgtca ctgcagaagg
      541 ctaaggacaa gtatgctgat gtgaaacagc tcatagcctc catcttccat gaacaccggg
      601 gatgttatgg gtaccggcgg atccattgtg agcttcagaa aagaggtctt aagttcagtg
      661 gcaaaacggt gcgaaaactg atgcaacagc tcggcctgaa gtctcctgtg cgcctgaaga
      721 aataccggtc ttaccgggga aatatgggac tcgctgcaga aaatatcctt cagcgacagt
      781 tcaaagcgga agctccctgt gagaaatggg tgacagatat caccgaattc agggctggcg
      841 gacaaaaact gtacctgtcc ccgatacttg atttgtttaa cggggaaatc gtggcgtggg
      901 aaacggcctg caggcctaca gaggagctgg tgaagcggat gttaaataag gggctggaaa
      961 gccttgctga aggggagaaa ccgctgctcc acagtgatca aggatggcat taccggataa
     1021 aaagttatca gtccgccctg gcggacagag ggttagtgca gagcatgtcg cgcaagggca
     1081 actgccttga taatgcggta atggagaact tctttggtca cctgaaagaa gaaatgtact
     1141 atcgccggga ctacagaa
//
LOCUS       DADSZD010000362         1126 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.364, whole genome shotgun sequence.
ACCESSION   DADSZD010000362 DADSZD010000000
VERSION     DADSZD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.364"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(13..900)
                     /gene="rhtA"
                     /locus_tag="J1Z28_004551"
     CDS             complement(13..900)
                     /gene="rhtA"
                     /locus_tag="J1Z28_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBA7719288.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGTLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT          336 a          287 c          271 g          232 t
ORIGIN      
        1 agaaatgtaa atttaattta tgtctaattc ttttattttg ctctctttgc gtactgtcag
       61 cgtagaaccc attgaagcgg cgatgatagc gccgagcgcc agcagctgta tgggtgtcag
      121 tgtttctccg aggaaaatca tcccggaaac ggcagccagc gccggttcca tgctcattag
      181 cgtaccaaat gtgcgtgttg gcaaacgggt gagggcaatc atttccagcg aataaggcag
      241 agcggtcgag agaatagcga cagccagacc caatggaata accgaccagt gccagagtgc
      301 ttcaccagcc tgaagcgttc caattggcac gaaaattaac gctgcaatca acgaaccaat
      361 tgccaccgtc gcagggccat gttccgctcc agcacgttgc ccacttaaaa tgtaaatagc
      421 ccaacaagcc ccggccccca gtgccagcgc acagccggtt aaatcgacat gggaaacgtc
      481 ttgccccagc ggtagcagga accacagacc aagaaccgcc agcacaaccc agacgaaatc
      541 taccgggcga cgagaagaga acagtgccac cgccagtggt ccggtgaact ccagcgccac
      601 cgcaataccc agcggtaccg tctgaataga aagataaaaa agataattca ttccacccag
      661 cgaaacgccg taaaacaaca gcggtaatcg ttgctctttg gcaaagcgca gtcgccatgg
      721 cttaaagaac gcgatgagga tcagcgtgcc taatgccaga cgcagcgcag tgacccctgg
      781 tgcgcccacc agaggaaaaa gtgacttagc taacgaggct ccaccctgaa tagacgccat
      841 ggcaacgagc aatatgacta ttggtaacca gaccggcatt ttacgtaatg aaccaggcat
      901 cctttctccc acaaatatct aaacctaagt aaagcgtgga gtgtactgga tatacccaat
      961 gctggttgag catttgttga aaaaattttc ccccgttttg actaaaatgc gccaggattg
     1021 atggaatcat tagtctggtg attaggaata atctggatga atgacaggga aaacatgcgt
     1081 aatacttacg cagttctctg aaaaagtgat ttaaatttag atggat
//
LOCUS       DADSZD010000363         1116 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.365, whole genome shotgun sequence.
ACCESSION   DADSZD010000363 DADSZD010000000
VERSION     DADSZD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.365"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            38..295
                     /locus_tag="J1Z28_004552"
     CDS             38..295
                     /locus_tag="J1Z28_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBA7719289.1"
                     /translation="MGLLSKDRYTSPGSMSIEHFRCLVEISSINSQKTIMAMEDYFVH
                     GKTRKEACERNNVAQSYFSISVKKFIKISNAVAQASKFYRR"
     gene            340..873
                     /locus_tag="J1Z28_004553"
     CDS             340..873
                     /locus_tag="J1Z28_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7719290.1"
                     /translation="MLKSTLGIAIAFSLCAVAHAANQGQGVVNFKGTVIDAPCGIAPE
                     SADQTIDFGQISKSHLKNDGISVKKDLDIKLVNCDFTDPTAKKTVSVTFSGTPVNGHT
                     DELGTAGDTGTAVVVSASDGSMVKFDGTTSSNATQLQDGDNTLKYTTWVKKSSAAGTE
                     VKEGDFTAVANFNLSYQ"
     gene            959..>1116
                     /locus_tag="J1Z28_004554"
     CDS             959..>1116
                     /locus_tag="J1Z28_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7719291.1"
                     /translation="MLRRMILSVLFLWCAVAQALPSASFPPPGMTLPEYWGEEHVWWD
                     GRASFKGQV"
BASE COUNT          321 a          192 c          264 g          339 t
ORIGIN      
        1 atgggtgtgg gtgtaaggga tgttattacg taatggaatg ggattgttat ctaaggatag
       61 atatacctct ccaggtagta tgtctattga acatttcagg tgccttgttg agatctcatc
      121 aataaatagt caaaaaacca ttatggctat ggaagattac tttgttcatg gcaagaccag
      181 aaaagaagca tgtgaaagga acaatgtcgc acagagttat ttttcaatca gtgtaaaaaa
      241 gtttattaag ataagtaatg cggttgctca ggcttccaaa ttttatagaa gataattttt
      301 agtggtagaa agtagtctag taataggagt gttatcttta tgttaaaatc aactttgggg
      361 attgctattg ctttttcatt gtgtgctgtt gcacatgcag ccaatcaagg gcaaggtgtt
      421 gttaatttta aaggaactgt aattgatgcg ccatgtggta ttgctccgga atcagcagat
      481 cagacaattg attttggtca gatttctaaa tcgcatttga aaaacgacgg tatctcagtc
      541 aagaaagatt tagatattaa attggtcaat tgtgatttta ctgatcctac agcgaaaaaa
      601 acagtaagtg tcacattctc cggtacgcct gttaatggtc atactgatga gcttggtact
      661 gctggtgata ccggtactgc tgttgttgta tccgcctcag atggttctat ggttaaattt
      721 gatggtacaa catcaagcaa tgcaactcag ttgcaggatg gcgataacac gctgaaatat
      781 acgacttggg ttaaaaaatc cagcgccgca ggtactgaag tcaaagaagg cgatttcact
      841 gcagttgcta actttaatct gagttatcag taagtaacat tatccggttt taaccatctc
      901 atacacagac cctgggattt cagttccagg tcttaccgat aagattaagg acgactgcat
      961 gttacgacgc atgatccttt cggtactgtt tctctggtgt gcagtggctc aagccctgcc
     1021 atcagcttct tttccaccac cggggatgac tttgcccgag tactggggag aagaacacgt
     1081 ctggtgggat gggcgggcca gctttaaagg gcaggt
//
LOCUS       DADSZD010000364         1100 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.366, whole genome shotgun sequence.
ACCESSION   DADSZD010000364 DADSZD010000000
VERSION     DADSZD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.366"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            487..>1100
                     /locus_tag="J1Z28_004555"
     CDS             487..>1100
                     /locus_tag="J1Z28_004555"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBA7719292.1"
                     /translation="MQSASKLIYRGKLLGSLPTVGEIHKEIAVSEETVTWISLQRDII
                     ANILLGKDPRLLVIVGPCSIHDVQAAVDYAKRLFVLQNKYLSKMYIVMRTYFEKPRTR
                     KGWKGIMHDPDLNGSYDVEKGIRYARQCLSSITTMRVATATEFLDPFLTPYIADLICW
                     GAIGARTTESQTHRQLASGLHCPVGFKNSTDGNINLAIDAIIAAR"
BASE COUNT          300 a          252 c          244 g          304 t
ORIGIN      
        1 ggcatgtttt tacggggtga tgctttcatt aacctgtaca cgaacttcta ttcctgcatc
       61 attacaggcc tacagccact acgccacttc cagcggatcg ccctccctgc gtacctcccc
      121 gccttcttta ttctataact gcagacaggt gctgccgtcg aggcgcacca caaaatcccc
      181 acggcagacc tgatgccggg accagcggca gtacgacgac ttctgcagta ctctgttctg
      241 ccagcagaga ggctgtgcca cggcccggtc agcttttgta agttccacag ctagccgcag
      301 ctcagggatt aagattcagg taatttacgg aaaacaagtg atttccgtac gggagagaag
      361 gcctaacgta tattatctca gaaatgacta tgaatcccca tatattgcca attacacagt
      421 aaatgattat cattatcatt tactgtgtgc aactgatggt ctgaaataga gcttttagga
      481 gacaagatgc agtcagctag taaattaatc taccgcggga agttgcttgg cagtttaccc
      541 actgtgggcg agattcataa agaaatcgca gtatcggaag aaaccgtaac atggatctct
      601 ctgcaacgag atattatagc gaatattttg ctgggaaaag atcctcgctt acttgtgatt
      661 gttggtccat gctcaatcca tgacgttcag gccgcagttg attatgcgaa gcgattgttc
      721 gtattacaga ataagtactt atcgaaaatg tacattgtaa tgcgcactta ttttgagaaa
      781 cctcgaaccc gtaaaggctg gaagggaata atgcacgatc ctgatctaaa tggaagttat
      841 gatgttgaga aagggatacg atatgctcgc cagtgtttgt cttcaatcac aacgatgcgt
      901 gtcgcaaccg ctacagaatt cctcgatcct ttccttactc cctatattgc tgatttaata
      961 tgttggggtg ctattggtgc cagaaccact gaatcacaaa cgcatcgtca acttgcgtct
     1021 ggtttacatt gtccagttgg atttaaaaac agtactgatg gaaatattaa cttagctatt
     1081 gacgctatta ttgcagcccg
//
LOCUS       DADSZD010000365         1074 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.367, whole genome shotgun sequence.
ACCESSION   DADSZD010000365 DADSZD010000000
VERSION     DADSZD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.367"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>1074)
                     /locus_tag="J1Z28_004556"
     CDS             complement(<1..>1074)
                     /locus_tag="J1Z28_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBA7719293.1"
                     /translation="FLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFI
                     CLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYC
                     PDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTL
                     DEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNS
                     VESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILAR
                     LEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDT
                     HNISLVMTEADKALREAKSEGGNK"
BASE COUNT          342 a          224 c          220 g          288 t
ORIGIN      
        1 tttgttgccc ccctcgcttt tcgcttcgcg taaggcttta tctgcttccg tcatgacaag
       61 cgagatatta tgcgtgtcac ccgttgaaaa aaccgtgcca atactgatgg tcatttgttc
      121 aggaacatca tatctgttac taaagccggt caggagttcg acatttttac gcatacgttc
      181 cgcaatgatt tttgcctggg cactattgag ttcggtaaat agcaagccaa acacctcacc
      241 ctccagtcgc gctaaaatat catcggggcg gatgctttgc tggataatat cgacaattga
      301 taccagcact ttatcaccca cacgatgtcc ccattgggca ttgatgcgtt tgaactgatt
      361 aatatcaacc agcatgacac aaaagtcctt aacaacaggt cgcgacaata atgactctac
      421 gctgttgaaa aagtagcgcc gattataaat attggtcagt acatcatgaa ctgccagttt
      481 gctggagagt tgcagttcct gaaaaatgtt ataaatcaga aaagacacaa caaacagttt
      541 actggaaact tcaatcccgc gactgatata ccagatggtg taattatact catccagagt
      601 cagtagaagt aagttgcagc atagcgatgc cagacataat aatgctatta acggccatag
      661 ttcgctggct aatcgtgtac gcatgataat aaagaacagt aaaaatgccc acaaacaaac
      721 caatattttt gtatagttga ttccccaggt cgcagtatgg ttgtccggac agtaatcgac
      781 gacatacaaa gaatagtcag cgttataact gctcagattg tgtgccagaa ggggaaaaac
      841 taaaaaagga atcagcgcca gcaacagaat gccagttttc ttcttattgt tgtcaaggat
      901 gttgtctttc ccataacaaa agagcgccag cgaggttaaa caaatgaaac tgagttggcg
      961 gaaaatatag aaaatagaaa catcattcga ctttgtctgg atcagtgtac tgccgttgat
     1021 tggtttatgg ataattacaa tggtttcaac taaatagata aggccactta aaaa
//
LOCUS       DADSZD010000366         1040 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.368, whole genome shotgun sequence.
ACCESSION   DADSZD010000366 DADSZD010000000
VERSION     DADSZD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.368"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            51..995
                     /gene="leuO"
                     /locus_tag="J1Z28_004557"
     CDS             51..995
                     /gene="leuO"
                     /locus_tag="J1Z28_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBA7719294.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          283 a          222 c          251 g          284 t
ORIGIN      
        1 tctgcattcc aataagggaa agggagttaa gtgtgacagt ggagttaagt atgccagagg
       61 tacaaacaga tcatccagag acggcggagt taagcaagcc acagctacgc atggtcgatc
      121 tcaacttatt aaccgttttc gatgccgtga tgcaggagca aaacatcacc cgtgccgctc
      181 atgctctggg aatgtcgcaa cctgcggtca gtaacgctgt tgcacgcctg aaggtgatgt
      241 ttaatgacga gctttttgtt cgttatggcc gtggtattca accgactgct cgcgcatttc
      301 aactttttgg ttcagttcgt caggcattgc aattagtaca aaatgaattg cctggttcag
      361 gttttgaacc cgcgagcagt gaacgtgtat ttcatctttg tgtttgcagc ccgttagaca
      421 gcattctgac ctcgcagatt tataatcaca ttgagcagat tgcgccaaat atacatgtta
      481 tgttcaagtc ttcattaaat cagaacactg aacatcagct gcgttatcag gaaacggagt
      541 ttgtgattag ttatgaagac ttccatcgcc ctgaatttac cagtgtgcca ttatttaaag
      601 atgaaatggt gttggtagcc agcaaaaatc atccaacaat taaaggcccg ttactgaagc
      661 atgatgttta taacgaacaa catgctgcgg tttcgctcga tcgtttcgca tcatttagtc
      721 aaacctggta tgacacggta gataagcaag ccagtatcgc gtatcagggc atggcaatga
      781 tgagcgtact tagcgtggtg tcgcaaacgc atctggtcgc tattgcgccg cgttggttgg
      841 ctgaagagtt cgctgaatcc ttagagttac aggtattacc gctgccgtta aaacaaaaca
      901 gcagaacctg ttatctctcc tggcatgaag ctgccgggcg cgataaaggc catcagtgga
      961 tggaagagca attagtctca atttgcaaac gctaactgaa tgcagaatag gtcagacatc
     1021 aatgtctggt ttattctgca
//
LOCUS       DADSZD010000367         1023 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.369, whole genome shotgun sequence.
ACCESSION   DADSZD010000367 DADSZD010000000
VERSION     DADSZD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.369"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>1023
                     /locus_tag="J1Z28_004558"
     CDS             <1..>1023
                     /locus_tag="J1Z28_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBA7719295.1"
                     /translation="AQAAEVYNKDGNKLDLYGRVTALHYFSDDKGDDGDKTYARLGFK
                     GETQINDQLTGFGHWEYQFSGNKAESEGTAGNKTRLAFAGLKFADLGSIDYGRNYGIA
                     YDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNTDFFGLVDGLNFAAQYQGKNE
                     RDDLQKSNGDGYGFSASYEFDGFGFVAAYTKSDRTGKQVNGLNGSKTIEVKDPLTGDV
                     TEKEVSVDSGSVAKGKYAEMWGTGLKYDANNLYLAAIYSETQNMTTFGDTGVADKAQN
                     LEMVAQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVKYIDVGATYYFNKNMSTY
                     VDYKINL"
BASE COUNT          277 a          181 c          268 g          297 t
ORIGIN      
        1 gctcaggcag cagaagttta taataaagat ggtaataagc tggatcttta tggtcgtgta
       61 actgctctcc attacttctc agacgataaa ggtgatgatg gcgataagac ctatgcacgt
      121 cttggtttta aaggtgagac tcagatcaat gatcaactga caggttttgg tcactgggaa
      181 tatcagtttt caggtaataa ggcagagtct gaagggacgg caggcaataa aacccgtctg
      241 gcatttgccg gtttgaaatt cgctgattta ggtagcattg attacgggcg taattatggt
      301 atcgcttatg atgtcggttc atatacagac gttctgccag aatttggtgg cgatggctgg
      361 acgcagactg ataacttcat gactgctcgt acttccggag tgctgactta ccgcaataca
      421 gatttcttcg gactggtgga tggtcttaac tttgcagccc agtatcaggg aaaaaatgag
      481 cgagatgacc tccagaagtc taatggtgat ggatatggtt tctctgccag ctatgagttt
      541 gatggttttg gttttgtggc agcgtatacc aagtcagatc gtacaggtaa gcaggttaat
      601 ggcctgaatg gtagcaaaac tattgaagtc aaagatcctt taacggggga tgtgacggaa
      661 aaagaagtgt cagttgattc cggcagcgtt gcaaaaggta agtatgctga gatgtggggg
      721 actggcctta aatatgatgc aaacaatctg tatctggctg caatttattc cgaaacccag
      781 aatatgacca cttttggtga taccggggtt gcggataaag cacaaaacct tgaaatggtt
      841 gctcagtatc agttcgattt tggcctgcgt ccatctctgg cttatctgca gtcccgcgga
      901 caggatgtta tggttggtgg tgtgaaccat ggcgaccagg atctggttaa atatattgat
      961 gtcggtgcga cttattactt taacaaaaat atgtccactt atgttgatta taaaattaac
     1021 ctg
//
LOCUS       DADSZD010000368         1010 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.370, whole genome shotgun sequence.
ACCESSION   DADSZD010000368 DADSZD010000000
VERSION     DADSZD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.370"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            148..>1010
                     /locus_tag="J1Z28_004559"
     CDS             148..>1010
                     /locus_tag="J1Z28_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719296.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAANYDTWGYHDNTTTDLV
                     WPYDTDNDGIADAGGMDPMGSDGNYVTYNGFVYYNNANGDFDTVFKGDTVNGTISTYY
                     LNHDYASGSVNQVDISNSVIHGAITSELPFGYYSITPAEVNAKGEVVTPQTYNGYNFY
                     NGITTDLDYNWYDGDVFTLNVSNSTIDDDYEALYFTDTYLDGDKSKSTNETFYTALGV
                     AVNLDVESNINITNNSRVAGIALSEGNTSNTTYTSEYHQWDNNINVSNSTVTSGSQTP
                     LEESNGFFGKSA"
BASE COUNT          306 a          215 c          200 g          289 t
ORIGIN      
        1 atatatgtcc gcaatcggac ttagtggaaa gaataattga accattcctc tataacgccg
       61 cataatcgta aacgcgaaac ataatatgtt ttctatgcat tgataattga tggatcaact
      121 tattacgtcc ctgaggaggg atgacaaatg cactcctgga aaaagaaact tgtagtatca
      181 caattagcat tggcttgcac tctggctatc acctctcagg ctaatgcagc aaactatgat
      241 acctgggggt atcatgacaa caccacgacc gatttagttt ggccgtatga cactgacaat
      301 gatggaattg ccgatgctgg cggcatggac ccgatgggtt cagacggtaa ctacgtaact
      361 tacaatggtt ttgtttacta taacaatgcc aatggcgatt tcgataccgt ctttaaaggc
      421 gataccgtta acggtaccat ttctacttat tacttgaatc atgattacgc tagcggttct
      481 gttaaccagg tagatatcag caattcagta attcatgggg caatcacctc tgaactgcca
      541 tttggttatt acagcattac tcctgcagaa gttaatgcta aaggtgaagt tgtaactcca
      601 cagacctata atggctataa tttctataac ggaatcacta ctgaccttga ctacaattgg
      661 tacgatggtg acgtattcac tctgaacgta tccaattcca ccattgatga tgactatgaa
      721 gctctttact tcactgacac atatcttgat ggtgataaat ctaagtcaac caatgaaacg
      781 ttctataccg ctctgggtgt tgcagtaaat cttgatgttg aaagcaatat caatattact
      841 aacaactccc gtgtagctgg tattgcgctg tctgaaggca atacaagcaa cacgacttat
      901 actagcgaat atcatcagtg ggacaacaac attaatgtgt ctaactccac tgtaacttca
      961 gggtcacaga ctccgctgga agaaagcaat ggcttcttcg gtaaatctgc
//
LOCUS       DADSZD010000369         1010 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.371, whole genome shotgun sequence.
ACCESSION   DADSZD010000369 DADSZD010000000
VERSION     DADSZD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.371"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..748
                     /locus_tag="J1Z28_004560"
     CDS             <1..748
                     /locus_tag="J1Z28_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HBA7719297.1"
                     /translation="GAVQLKTGAVNGAKAQALTINKDEVNSTVDLTLTKQTGTGNRFV
                     LQNSGNAELPFSVRVWGSSTRQNVFEVGTSAAYLFYAQKTSAGQLFDVNGAINCTTLN
                     QSSDRDLKDDILVISDATKAIRKMNGYTYTLRENGMPYAGVIAQEVMEAIPEAVGSFT
                     HYGEELQGPTVDGNELREETRYLNVDYAAVTGLLIQVARETDDRVTALEEENTTLREN
                     LATAGTRITTLENQVSELVALVRQLTESEH"
     gene            803..955
                     /locus_tag="J1Z28_004561"
     CDS             803..955
                     /locus_tag="J1Z28_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001316948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBA7719298.1"
                     /translation="MEIATEEETSLLEVWKKYRVLLNRVDTSTAPDVDWPAAPKCNSL
                     ELITCE"
BASE COUNT          292 a          208 c          255 g          255 t
ORIGIN      
        1 cggcgcggtt caactcaaaa caggtgctgt taacggggct aaagcgcagg cgctcactat
       61 caacaaggat gaggttaact caactgttga tttaactctt acaaaacaaa cagggactgg
      121 caatcgtttt gttttacaga actcaggtaa tgcagaacta ccgttttctg tcagggtgtg
      181 gggttccagt actcgacaaa acgtttttga ggttggaacg tctgctgcgt atctgtttta
      241 tgcgcaaaaa acgtcagcag gccagttgtt tgatgtaaat ggcgctatta attgcacaac
      301 gctgaatcag tcatcagacc gcgaccttaa agacgatatt ctcgttatca gcgacgcgac
      361 gaaagcaatc cgtaaaatga acggatacac ctacacgctc agggaaaacg ggatgcctta
      421 tgctggcgtt attgcacagg aagtaatgga ggcgatacca gaagctgtgg gatcgtttac
      481 tcattacggt gaagagttgc agggaccaac ggttgacggc aatgaattac gtgaagaaac
      541 acgttatctg aatgttgact acgccgccgt gactggctta cttattcagg tcgcccgtga
      601 aacagatgat cgcgttaccg cgctggaaga ggaaaataca acgttacgtg aaaatctggc
      661 aacagcaggc acccggatca ccactctgga aaatcaggta agcgaactgg ttgcacttgt
      721 ccggcagtta acagaaagcg aacattgata tccttcaagc cctgaaggag gctgttcctg
      781 gtacgttcag actgttgttg agctggaaat cgcaacggag gaagaaactt cgttgctgga
      841 agtctggaaa aagtatcggg tgttgctgaa ccgcgttgat acatcaactg cacctgatgt
      901 tgattggcca gcagcaccga agtgtaattc attagaactc atcacctgtg aataatatct
      961 gtttttgagt aagttagtaa taatcactta aatgtgttat aagtttgtta
//
LOCUS       DADSZD010000370          994 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.372, whole genome shotgun sequence.
ACCESSION   DADSZD010000370 DADSZD010000000
VERSION     DADSZD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.372"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..886)
                     /locus_tag="J1Z28_004562"
     CDS             complement(<1..886)
                     /locus_tag="J1Z28_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719299.1"
                     /translation="MGLFYYKSYASTQIRELVSGSYNLAIYSNTGKVLGLGSFLASKL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGF
                     ENDPVFRVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTD"
BASE COUNT          318 a          239 c          178 g          259 t
ORIGIN      
        1 cgtcagtttt tgaccccgca aaatgcgcac tccagcttcc cagcgggagt gcaagcacag
       61 aaaaggggat aatattccag aggaatgaac cataaaggtc tgtgtataaa acaatgttat
      121 caggagaaga gtcatattca cgatccgggg tcgtcaagcc cggccccatt gctttaactg
      181 cctcccagaa cccggctcca tctcccagca cacgaaatac cggatcgttt tcaaaaccga
      241 tatttaatac cttcccatcc ccgtcaaata ccagcgggga agcccaggct atatagtcag
      301 aattagcata aaaaccatca gccagagact cactgagtct ggccataatg ttagtcaacc
      361 caccccccag gctatagcct gtgacaataa catcttcacc acttaaacca ttactgattg
      421 caaagtctct gatactttga agcaggtttt ctgcttcaga cagcgctttt ccattattga
      481 gctgtgtata atcaacaata tcgcagatat catttgttcc acagaatgat attgcaattt
      541 tctgtagatt ctgttgagca tcaaacaacc caacaacttt caactgagga ccaaatcctg
      601 acgcagggtt tcctgtaacc ggactgggaa aacgataaaa ccctcttgaa tcgacatatt
      661 ccgaagataa cctgagttct tccactgtaa tatcacgcca gccttcatta agaacatcgg
      721 tagaataatc aatcctatct gggtgctttg caaataactt ggatgccaga aaacttccta
      781 atccaagaac ctttccagtg ttggaatata tagcaagatt ataagagcca gaaacaagct
      841 ctctaatttg tgttgacgca taagatttgt aataaaacaa ccccatacaa acaccttgat
      901 gttgaaaaaa aactggacag aaagagcccc ataggggctc ttttataaga ttacattaca
      961 ttcgtaatgt gttcatcgat taatatttta atgt
//
LOCUS       DADSZD010000371          993 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.373, whole genome shotgun sequence.
ACCESSION   DADSZD010000371 DADSZD010000000
VERSION     DADSZD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.373"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            124..948
                     /gene="gadX"
                     /locus_tag="J1Z28_004563"
     CDS             124..948
                     /gene="gadX"
                     /locus_tag="J1Z28_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBA7719300.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHAHKKVPSLLVHHCTRGIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGPAASMAAQGIFYGTDRSAEGIRL"
BASE COUNT          285 a          184 c          218 g          306 t
ORIGIN      
        1 tcatcgctca ttatcatcct gttttcccgc ttcgtttaaa tttatttatc aatcaatttg
       61 acttaagagg gcggcgtgct acattaataa acagtaatat gtttatgtaa tattaagtca
      121 actatgcaac cactacatgg taattgtcta attgcgtacg caagacataa atatattctc
      181 accatggtta atggtgaata tcgctatttt aatggcggtg atctggtttt tgcggatgca
      241 agccaaattc aagtggataa gtgtgttgag aatttcgtat tagtttcaag agacacgctt
      301 tcattatttc tcccgatgct caaggaggag gcattaaaac ttcatgcaca taaaaaagtt
      361 ccttcattac tcgttcatca ctgtaccaga ggtattcctg tttttcagga agttgcgcaa
      421 ctatcgcaga ataagaatct tcgctatgca gaaatgctac gtaaaagggc attaatcttt
      481 gcgttgttat ctgtttttct tgaggatacg cagtttatac cgttacttct gaacgtgttg
      541 caaccgaata tgcgaacacg agtttgtacg gtcatcaata ataatatcgc ccatgagtgg
      601 acattagccc gaatcgctag cgaattgttg atgagtccaa gcctgttaaa gaaaaaattg
      661 cgcgaagaag gtacgtcata ttcacagttg cttactgagt gtaggatgca gcgtgcttta
      721 caacttattg ttatatatgg ggtttcaatt aagcgagttg cagtatcctg tggatatcac
      781 agcgtgtcgt atttcattta cgtttttcga aattattatg ggatgacgcc cacagagtat
      841 caggagcgat cggcgcagga attaccgaac tgtggcccgg cggcaagtat ggctgcgcaa
      901 gggatttttt acggcactga ccgttctgcg gaaggaataa gattatagag ttttactcag
      961 acataaaaaa aacccggcat aggggaccgg gaa
//
LOCUS       DADSZD010000372          986 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.374, whole genome shotgun sequence.
ACCESSION   DADSZD010000372 DADSZD010000000
VERSION     DADSZD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.374"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(90..653)
                     /locus_tag="J1Z28_004564"
     CDS             complement(90..653)
                     /locus_tag="J1Z28_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease PIN"
                     /protein_id="HBA7719301.1"
                     /translation="MNRATIGFYLAVVLGSCSMNGVSQADELLTRDDFFVADESRHQW
                     VTEHNGRTGALNVKGALVSSPCILDTPEVNFPLQKDKGRYVLNLKVSRCGDGHSDIPE
                     RQSTEHGNIVVKQSAVLKTGKDQILLSAWRNGGLSRKNLQHGDNQITYYINEAQYQKI
                     AKTQMPVTAHKTSDSNRGVMHLNIMYE"
     gene            complement(690..>986)
                     /locus_tag="J1Z28_004565"
     CDS             complement(690..>986)
                     /locus_tag="J1Z28_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBA7719302.1"
                     /translation="IKAKPNAVWQDQLVLNKTSGGYRIDNPTPYYVTVIGIGGSEKQA
                     REGEFDAVMLAPKSTQMVKSGTYNTPYLSYINDYGGRPVLQFSCGGSRCTAVKK"
BASE COUNT          240 a          262 c          180 g          304 t
ORIGIN      
        1 tctcaaatct ttctcctgat ataaaacaag gaggtaacaa gatggttatt aagacacaac
       61 gagataataa tgatgccagc gtactgtgcc tattcataca ttatattcaa atgcattacc
      121 cccctgttac tgtcagatgt tttatgcgca gtaacaggca tttgtgtttt agctatcttt
      181 tgatattgag cttcatttat ataataagtg atctgattat caccatgttg taaattcttt
      241 ctacttaatc caccgtttcg ccatgcggat agcagaattt gatctttgcc tgttttcaac
      301 acagcgcttt gcttcacaac aatattccca tgctctgttg actggcgttc cggtatgtca
      361 gaatgcccat caccacaacg tgatacctta agattgagta catatctccc tttatctttc
      421 tgcaggggga agttaacttc tggcgtatcc agtatgcacg gagatgacac taatgcacct
      481 ttgacgttca gagctcctgt ccgcccattg tgctcagtga cccactgatg acggctttca
      541 tctgctacga aaaaatcatc ccgcgtcaac aattcatccg cctgtgagac accgttcata
      601 ctgcaactcc ccagcacaac tgccagataa aatcctatcg ttgccctgtt catctccgac
      661 acctccggac aattcttttt cctgctttct tatttcttca ctgcggtaca gcggctacca
      721 ccacaggaga actgcagcac cggacgtccg ccgtagtcat tgatgtaact caggtatggt
      781 gtgttgtatg ttcctgattt caccatctgc gttgacttcg gtgccagcat cacagcatca
      841 aattcacctt ctctggcctg cttttcgctg ccgccaatgc caattacagt cacgtaatac
      901 ggtgtcggat tatcaatccg ataccctccg ctggtcttat tcagcaccaa ctgatcctgc
      961 cataccgcat tgggctttgc tttaat
//
LOCUS       DADSZD010000373          975 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.375, whole genome shotgun sequence.
ACCESSION   DADSZD010000373 DADSZD010000000
VERSION     DADSZD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.375"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..408
                     /locus_tag="J1Z28_004566"
     CDS             <1..408
                     /locus_tag="J1Z28_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBA7719303.1"
                     /translation="NAASTRAGESKIKHKISHDVFDPYPAALHGQFDSISMFYLLHCL
                     PGNISTKSCVIRNAAQALTDDGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKD
                     SEEGLTHILSEHFENVKTKVKGTVVMFSASGKK"
     gene            complement(463..768)
                     /locus_tag="J1Z28_004567"
                     /pseudo
     CDS             complement(463..768)
                     /locus_tag="J1Z28_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            822..>975
                     /locus_tag="J1Z28_004568"
     CDS             822..>975
                     /locus_tag="J1Z28_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBA7719304.1"
                     /translation="MRWNGTRDLQKNQEKRLIDESPEPLKSVVKFALVTVLRKSNIIN
                     LEWQQID"
BASE COUNT          289 a          236 c          209 g          241 t
ORIGIN      
        1 aacgcggcat cgacaagggc tggggaatca aaaattaaac ataaaattag ccatgatgtt
       61 tttgatcctt atcccgcggc gttacatggt caatttgatt ccatttccat gttttacctt
      121 cttcactgcc tgcctggaaa tatatctaca aaaagctgtg taatacgcaa tgcggcgcag
      181 gccttaactg acgatggaac tctatacgga gccacaattc ttggtgacgg cgttgtgcac
      241 aatagcttcg gtcaaaaact gatgcgcatt tacaatcaga aaggcatctt ttcaaacaca
      301 aaagattccg aagaaggctt aacacatata ctctcagagc atttcgagaa tgttaaaacc
      361 aaggttaaag gtactgtagt aatgttttcc gcttcaggga aaaaatagca tccagccgca
      421 gcacgttctt gcgcacgacg tgctgcggca taatcccaat gattactctc tgacagggtt
      481 cacaggccac tcaatatcag gggcagttga tgtatcaact cggttcagca acacccgata
      541 catcttacag gcttccagca acgaggtttc ttcctccgta tcctttatcc atgatgtgcc
      601 gttctacttc tgaaactccc ctgccgggga taaccaggta acattttccg gtaacggacc
      661 gagctcagaa ataaataacg cgtcccctga tgccacgtca taaaccgttt taccccgatg
      721 gtcttcaacg agatgccacg atgcctcatc actgttgaaa acaaccacga agccagccgg
      781 aatatctggg caatatcagt actgtttgca ggcaaacctg tatgaggtgg aatggcacaa
      841 gagacttaca aaagaatcag gaaaaacgcc tgattgatga gagccccgag ccactgaaat
      901 ctgtcgtcaa gtttgcgctg gtaactgttc tgagaaagtc gaacatcata aatctggagt
      961 ggcaacagat tgaca
//
LOCUS       DADSZD010000374          963 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.376, whole genome shotgun sequence.
ACCESSION   DADSZD010000374 DADSZD010000000
VERSION     DADSZD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.376"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>963
                     /locus_tag="J1Z28_004569"
     CDS             <1..>963
                     /locus_tag="J1Z28_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBA7719305.1"
                     /translation="KRVKKVVPDLQLSFEHIARRWHASNKQWAQSHSDKVLKSLETHV
                     FPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRYAVQSGIIRYNPA
                     LDMAGALTTVKRQHRPALNLSRLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSSELRY
                     ARWSEIDIDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQAVAILTELQTWAG
                     ENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRD
                     AVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANRER"
BASE COUNT          265 a          236 c          229 g          233 t
ORIGIN      
        1 aaaagagtta aaaaagtagt ccctgattta cagctctctt ttgaacatat tgcacgacgc
       61 tggcatgcca gtaataaaca atgggcacaa tcacacagcg ataaagtact caaaagcctc
      121 gagacacacg ttttcccctt tatcggcaac cgggatatca caactctcaa taccccggac
      181 ctgcttatcc ctgttcgtgc tgcagaagca aaacaaattt atgaaatcgc tagtcgtctg
      241 cagcaaagaa tatctgctgt aatgcgttac gccgtacagt ctggcatcat cagatataac
      301 ccggctctgg atatggctgg tgcattgacc acggtaaaac gccagcatcg ccccgctctg
      361 aatctttcac gcctgcctga acttctgtcg cgtattgacg gttataaagg ccagcctgtc
      421 acccggcttg ccgttatgct gaatttactg gtttttattc gttccagtga actcagatat
      481 gcccgctggt ctgaaattga tattgacaat gccatgtgga ctattccagc cgaacgcgaa
      541 cctctgcctg gcgtaaaatt ctcacaccgg ggctccaaga tgcggacacc acatcttgtg
      601 ccactcagta aacaggctgt agccatactg acagaacttc agacatgggc aggtgaaaat
      661 ggtctgatat ttacgggtgc acatgacccg cgtaaaccaa tcagtgaaaa tactgtaaat
      721 aaagccctga gggtgatggg gtatgacaca acccaggaag tctgtggcca tggattccgg
      781 gcgatggcgt gcagtgcatt gattgaatca ggtttgtggt cccgcgatgc tgtggaacgt
      841 cagatgagcc atcaggaacg taacggtgta cgtgctgctt acattcataa agcagaacat
      901 ctggaagaac gccgcttgat gctacaatgg tgggccgatt ttctggatgc aaacagagaa
      961 aga
//
LOCUS       DADSZD010000375          958 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.377, whole genome shotgun sequence.
ACCESSION   DADSZD010000375 DADSZD010000000
VERSION     DADSZD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.377"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(107..>958)
                     /gene="xapR"
                     /locus_tag="J1Z28_004570"
     CDS             complement(107..>958)
                     /gene="xapR"
                     /locus_tag="J1Z28_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HBA7719306.1"
                     /translation="LRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQLFI
                     RHSRSVVLTHAGKILMEESRRLLVNANNILARVEQIGQGEAGRIELGVVGTAMWGRMR
                     PVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGFTSLRLHESA
                     FLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIREVN
                     EPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKERIPADLYIVYETQQVTPALVK
                     LLAALAQ"
BASE COUNT          240 a          272 c          219 g          227 t
ORIGIN      
        1 aaaaaagcgc cctaaaggcg cttttttgct atctgcaatt tgcgaaattg cctgatgcgc
       61 ttcgcttatc agaccaacta tgtccggcag ttcccgtgcc tctcatctac tgcgccaacg
      121 ccgccagcag tttaaccagc gcgggcgtca cctgctgcgt ttcataaaca atatataagt
      181 ctgccgggat gcgttctttg agcggacgga atatgacacc gggccagttc atctgcgcgt
      241 agctgtccgc tatcaatgtg ataccaatac ccatactgac catggcgagt accgtttgcg
      301 gttcattaac ttcgcgaata acaaccggtg aaaatcccac ctgctggcaa actcgctgca
      361 aaaaatccca gtcagtgtac acgggcggca ttgtaacgaa atactcgtca cgtagcgctt
      421 ccagcgggac ggtggaaaat gatgagagat gatgctcttc aggcatcgcc accagaaacg
      481 ccgattcatg caaccgtaag ctggtaaaac cagtcggtgg ttctgtcgcc attcgccaga
      541 tcccggcatc aagttcgcgg cgttccagca aggccatttg catcgcgggc atcttttcgc
      601 gaaacagaac ttcaacgtta ggattttctc taaggaatcg ccgcataacc gggcgcatcc
      661 gtccccacat tgccgttccc acaaccccga gttcaatccg ccctgcttct ccctgaccta
      721 tttgttcaac ccgagccaat atattatttg cattcaccag caatcgacgc gattcttcca
      781 tcaagatttt gcccgcgtgt gtcagtacga cgctgcgcga atggcgaata aaaagctgcg
      841 tgccgagttg attttccaac tctttaatat gaatactgag cggaggctga gacatattta
      901 aacgcgctgc tgcgcgacca aaatgcaact cttccgctac ggcaagaaaa taacggag
//
LOCUS       DADSZD010000376          953 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.378, whole genome shotgun sequence.
ACCESSION   DADSZD010000376 DADSZD010000000
VERSION     DADSZD010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.378"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(81..935)
                     /locus_tag="J1Z28_004571"
     CDS             complement(81..935)
                     /locus_tag="J1Z28_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBA7719307.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFVAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMMEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT          249 a          252 c          225 g          227 t
ORIGIN      
        1 ccgttggaaa tagaaatagt tgaaaggctc acaactcccg gaatgcggat tatctttgcc
       61 ctgatttctg gcggttttat tcagggtgca agctgtctga ccctttcccg atattctccc
      121 ggagtacaac caaactcgcg gacaaacgcc ttgtgaaaag atgattcact ggcatagcct
      181 actgactcag cgatcaccac aacagggagc atttcccggg aaaacatctg ggccgctatt
      241 tgtagacgca actttgttaa tacagccagc ggcgtggttc cggaaacatc acggaaaagc
      301 tgggcaaaac ttgcccggga catgtgggcg atgctggcca gcgattcgac ggtccaggcg
      361 tgtccaggca tttccatcat ttgctgtatt accgcaccaa gacgtggatg cagaagcaaa
      421 ctgagaatgt ttttctctgt attaacctgt gcaatccatt cacgcaccgc gagggtaaag
      481 aatgtagcgc agatctggct acacagagca tccactcccg gcattgccga tctggattcc
      541 tgttgtaaaa acgggatcgc ctccctcagc cagttatatt ccacgctgtg gttaaccggt
      601 gctacaaaca gcgtttccgg caaagacgtt aaaaaataac gcgccgaatg ttgcagccga
      661 agagttccac agacaataca ggtcgattca ttatcgacat gactcaggcg atgagctgaa
      721 ttttgtggta gcaggaccac atttcccggg cgtaatgtaa aaatctctcc cgtcggcatt
      781 tccagcttcg ccgctccttg cgttaacgca tgccaacgaa taaccgacaa ttccccggca
      841 ccatgtggaa gttgccagtc acttcctaac acgcaattct tatcgatcgt tccttgtgga
      901 gcgttaagca tcaacaaacg gctaagagca tccatatcag actcctgagt ggt
//
LOCUS       DADSZD010000377          940 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.379, whole genome shotgun sequence.
ACCESSION   DADSZD010000377 DADSZD010000000
VERSION     DADSZD010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.379"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>940
                     /locus_tag="J1Z28_004572"
     CDS             <1..>940
                     /locus_tag="J1Z28_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719308.1"
                     /translation="KAHLEKALNTVYQPNDDLYASYKWGIGKPTGNPEASTTTWREVL
                     NQNGVSGFSVDNNHAGEFRYTYNGLEGSDIISTGYSIGGTGRTMITRNDMVINTNGGD
                     DIIAVGMDLGRSYSAGYTDKKYVTSTGDGDDILIVGLGNNNVGIHLKDDASLRISDRD
                     DVISDGELLLPSNSYDHGSGGIISSTTITMGDGDDTLLALGYEGFGKAIVNTDIDLGA
                     GNDTIHVNGDVEAVSIKGGSGMDILAISHGTLSTKDFSGFEVIDLGDNNGGVNIIASD
                     LVKSGVDAIEGGTLKIIGSSNSRVNLDGDDWKSDGVQ"
BASE COUNT          281 a          133 c          234 g          292 t
ORIGIN      
        1 aaagctcatt tggaaaaggc actaaatact gtctatcagc caaatgatga cctttatgct
       61 tcctataaat ggggcattgg taaacctact ggtaatccag aagcaagcac tactacatgg
      121 cgtgaagttc ttaatcaaaa tggtgtttct ggcttcagtg ttgataataa tcatgctgga
      181 gaattccgat acacttataa tggattggag ggcagcgata tcatatcaac aggctattct
      241 attggtggca ctggcagaac aatgataact cgtaatgaca tggttattaa tactaacggt
      301 ggcgatgata ttattgctgt tggtatggat cttggtagaa gctatagtgc tggctatacc
      361 gacaaaaaat atgtgacatc aacgggagat ggtgatgaca ttctgattgt cggcctaggt
      421 aataataatg ttggtataca tctgaaagat gatgcaagtt tacgtattag tgatagagat
      481 gatgtgatta gtgatggaga actgttatta ccatcaaatt catatgatca tggtagtggc
      541 ggtattatat cgtctaccac cataactatg ggtgatggtg atgatactct tcttgcttta
      601 ggctatgagg gatttggtaa ggctattgtt aataccgata ttgacttagg tgcaggaaat
      661 gacactattc atgttaatgg tgatgtggaa gcggtaagta ttaaaggtgg ctctggtatg
      721 gatattctgg ccattagtca tggaaccctt tcaactaaag atttctctgg gtttgaagtt
      781 attgaccttg gtgataataa tggaggggtg aatataattg catctgacct tgtgaaaagt
      841 ggagttgatg ctatagaggg aggcacactt aaaattattg ggtcatctaa ctcgagggta
      901 aacttagatg gagatgactg gaagtctgat ggagttcaat
//
LOCUS       DADSZD010000378          934 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.380, whole genome shotgun sequence.
ACCESSION   DADSZD010000378 DADSZD010000000
VERSION     DADSZD010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.380"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..905
                     /locus_tag="J1Z28_004573"
     CDS             <1..905
                     /locus_tag="J1Z28_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7719309.1"
                     /translation="QSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKV
                     GNTLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQKVINGWN
                     KKLADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADIN
                     AGTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTLQK
                     PVGQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAGNNMSNVV
                     IDYFKKGHKITPAQVNEMRVEVSK"
BASE COUNT          288 a          206 c          209 g          231 t
ORIGIN      
        1 gtcagtccag cattggcaat acagatgtta gacgtgcgct ggataaagat attcaaaccg
       61 caagcaaggt gaaaggattg gatattctga ttaccgggca tgcccacgtc ggaacgccag
      121 aaccaatcaa agtcggcaac accttaatcc tttcgactga cagtggcggt atcgatattg
      181 gtaaattaat ccttgatgtc aaccctacag ctcggactca taaagtgaag agctttgagc
      241 tgaaaacggt ttatgccgat gaatggatac ccgatccaac cacacaaaaa gttatcaatg
      301 gctggaataa aaaactggcg gatcttgtac gccaaccagt cggtgaatca tcgattgcat
      361 taactcgtgc ctacggtgag tcatcgcaac ttggcaacct gtttaccgat gcaatgcttg
      421 tcgctgcacc gactgcacaa attgcgttga taaactccgg cagtttacgt gctgatataa
      481 atgcaggcac gattaccttt ggtgacatta ccagtacatt ccccttcaag aatgaactta
      541 ctgaaatgga tctcagcggt aaggatctgc gtaacctact ggaacacggg gcatcgctta
      601 ccaatggcat cctgcaaatg tcaaaaggcg cagaaatgcg ctataccctg cagaaaccag
      661 taggtcagcg cattgtatct tttaaaatta atggtgaaga gatcgttgat acgaatattt
      721 accatgttgc gactacgact ttccttgcac ttgggggcga tggttttctg gcattcaaag
      781 aagggaaaaa tgtccaggtc cgcgccggaa ataacatgtc caatgtcgta atcgattatt
      841 ttaagaaagg ccacaagatt actcctgcac aggtaaatga aatgcgggta gaagtaagca
      901 aataacaaaa acttataata ccattcattc actg
//
LOCUS       DADSZD010000379          921 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.381, whole genome shotgun sequence.
ACCESSION   DADSZD010000379 DADSZD010000000
VERSION     DADSZD010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.381"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            359..628
                     /locus_tag="J1Z28_004574"
     CDS             359..628
                     /locus_tag="J1Z28_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719310.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDCNGSQLIRDFMRQTVERQ
                     HNIWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            625..807
                     /locus_tag="J1Z28_004575"
     CDS             625..807
                     /locus_tag="J1Z28_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBA7719311.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLF
                     SLSFISVRVHDNIITV"
BASE COUNT          248 a          188 c          251 g          234 t
ORIGIN      
        1 aagtaacata actcattcat ctatttccta gtttctctgt atattaaaag agaatggtaa
       61 tcattaccat gttaaggtgt catatcgttt acataaagtc aagatatatc agtgtggcaa
      121 gcctggcagg attctctagg gaagggcagg catactcagt gcatagctga tgcacggatg
      181 acggcaaggg ggaggaggct ggtgactttc ggacgactgt agcggcttac atcgggcttc
      241 ggcggtatca tatgaaacag cggagtgccg ccttccatgc cgctggcgcg gcatatccta
      301 gtgacgatat ctgaatcgtt atatactgtg tatatacgtg gtaatgacgg ggtgataaat
      361 ggcacaggtt aatatgagtt taagaattga tgctgaactg aaggatgctt ttatggccgc
      421 tgcaaaaagc atggactgta atggctctca gttaatccgg gattttatgc gccagaccgt
      481 ggaacggcag cataatatct ggttccgtga ccaggttgag gcaggacgtc agcaactcga
      541 gcgcggcgat gtgcttcccc atgacatggt cgaatcttcc gctgctgcat ggcgggatga
      601 aatgagcagg aaagtcgccg gtaaatgatg gagatattct ggaccatgct ggccagccag
      661 gacaggaagc gcattcgtga gtacattgcg gagcagaacc tgatagccgc cattgaattg
      721 gacgaacgga ttggttgttc agccagcctc ctgttctccc tttcttttat cagtgtacgg
      781 gtgcatgaca acataatcac ggtatgataa taataatcaa taacaataag ttgtgtcacg
      841 tttacatcat ggaacggagg tgctcctgtg acttttgagc agaaaaaagc ccgggccatt
      901 gccctgatgg acagcaaaaa g
//
LOCUS       DADSZD010000380          911 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.382, whole genome shotgun sequence.
ACCESSION   DADSZD010000380 DADSZD010000000
VERSION     DADSZD010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.382"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(117..428)
                     /locus_tag="J1Z28_004576"
     CDS             complement(117..428)
                     /locus_tag="J1Z28_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBA7719312.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ELACLQAHKIQTALISQDEGCGKIEVNLILVHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(456..>911)
                     /locus_tag="J1Z28_004577"
     CDS             complement(456..>911)
                     /locus_tag="J1Z28_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBA7719313.1"
                     /translation="YPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMS
                     FIPGIFNINEPIMFGLPIVLNPIMMVPFILVPIVNCAIGYFFVSMEIIPPVAYAVPWT
                     TPGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT          290 a          208 c          179 g          234 t
ORIGIN      
        1 gaaacaatgc tgtaataata gaaaaagaca aaaggctgac gttaatcgtc tttatattca
       61 tgacttatcc ttaagagtgt aataatttcc tccagctatc gcactggagg aaaaaatcag
      121 gcatggagct cttttcttaa tgaaataatt tccctggcta aatcctggca taatattgca
      181 ttcatcaaat gatcctgtgc atgaaccaga attaaattaa cttctatttt gccacaacct
      241 tcatcctgac taattaacgc ggtctgaatc ttatgtgcct gaagacaggc taattccgaa
      301 ctggccatta gctgagttgc gccctgccag tctttttgcc cggcacaacg tatggcttcc
      361 atcgcatcag aacgtgcctg tcccgcgttg atcagtaact ccataacaag ttcttcatct
      421 gcaaacatca acatctcctt cctgtgtaaa tgggattatc cgttagttgt catgttattg
      481 accttgttgg ctgcaataac aaaaggtaaa tagatcattg tcgccacacc taaacataaa
      541 aaacccacca gtaacgccag ccagtttccc ccggttccga ggaaagcaat taaaggtccg
      601 ggcgtagtcc agggcacggc ataagcaacc ggtggaataa tttccatcga aacaaagaag
      661 taaccaatgg cacagttaac aatgggaacc agaataaacg gcaccatcat gatggggtta
      721 agtacaatag gaaggccgaa cattatcggt tcattgatat tgaaaatacc ggggataaat
      781 gacatttttg ccagatcacg gtattccgca cgcttagaag cgatgaaaat agccaacaat
      841 aatcccaacg tcatacctga accgccgcag ttggcgaatg catcattaat agaagtccag
      901 gtaatcggat a
//
LOCUS       DADSZD010000381          909 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.383, whole genome shotgun sequence.
ACCESSION   DADSZD010000381 DADSZD010000000
VERSION     DADSZD010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.383"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..432
                     /locus_tag="J1Z28_004578"
     CDS             <1..432
                     /locus_tag="J1Z28_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBA7719314.1"
                     /translation="EGNHTMAYRDGSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVN
                     AIERDKALAWVERNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGAC
                     EAIRWWIKDGGRDCRIRSNNCYGQVIRRDQESALTCWGIEQ"
     gene            429..>909
                     /locus_tag="J1Z28_004579"
     CDS             429..>909
                     /locus_tag="J1Z28_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HBA7719315.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     "
BASE COUNT          259 a          192 c          244 g          214 t
ORIGIN      
        1 gaaggtaacc acacaatggc ataccgcgat ggttctggca tatggaccat ctgtcggggt
       61 gccacagtgg tggatggaaa aaccgttttt cccaatatga aactgtcgaa ggaaaaatgc
      121 gaccaggtca acgccattga gcgtgataag gcgctggcat gggtggagcg caatattaaa
      181 gtaccactga ccgaaccaca aaaagcgggt atcgcgtcat tttgtcccta taacattggt
      241 cccggtaagt gtttcccgtc gacgttttat aagcggctga atgctggtga tcgtaaaggt
      301 gcatgcgaag cgattcgctg gtggattaag gatggcggac gcgattgccg cattcgttca
      361 aataactgtt acggtcaggt tattcgtcgt gaccaggaga gcgcattaac ctgctggggg
      421 atagaacagt gaatcagata ttcatggtga tttttctcgt gttgtcagga tttatcgtcg
      481 gaaatgtctg gagcgacaga ggatggcaaa aaaaatgggc ggaacgtgat gctgccgcat
      541 tatcacaaga ggtaaatgct caatttgctg ctcgaataat tgaacagggg cgaactatag
      601 cccgtgatga ggctgttaaa gatgcacaac agaaatctgc tgaaatttct gccagggctg
      661 cttatctgtc tgatagtgtt aaccagttgc gtgccgaagc aaaaaaatat gccatacgcc
      721 ttgacgcagc gaagcatacc gcagatcttg ccgctgccgt cagaggcaaa acaaccaaaa
      781 ccgccgaagg aatgctcacc aacatgctcg gagatattgc agcagaagct cagctttatg
      841 ctgaaattgc tgacgaacgc tacatcgcag gagtgacttg tcaacagatc tatgaatctt
      901 taagagata
//
LOCUS       DADSZD010000382          907 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.384, whole genome shotgun sequence.
ACCESSION   DADSZD010000382 DADSZD010000000
VERSION     DADSZD010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.384"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(127..759)
                     /locus_tag="J1Z28_004580"
     CDS             complement(127..759)
                     /locus_tag="J1Z28_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBA7719316.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT          224 a          213 c          182 g          288 t
ORIGIN      
        1 aaatgcgagt ttatagaacc gcgagcgcca aataatttta tgaccacaac ctgtcgtatt
       61 gtggtcataa aaaattgtcg actggaggga gttaaatcct ttacctcaat atattgcctg
      121 tcaggtttag ttcacaaaca gtgcttcgct atcaacgatg attccatctt ccgcaccttt
      181 aagcgccagg tttgcctcgt agtattgctt tttatcaagc agtttctgcg catcagcaag
      241 tgcattacga gtctgtttaa gcggcatcaa atattggttt tccattacac caacaccggc
      301 caggcgaagc tcttccattg cacccttttt atcccctttc gccatttttt cgttggcaat
      361 ttttatcgcg gcttcttttt cgggtgtcgc tacataactc tcagaaattc cgacagatgc
      421 attaatgaca atatattggt catcattgac gttggtcttt ttacctggct tagcgaactt
      481 tgcccattcg gtactatcat ctgacagcaa agcggaagct tcattggtca gttcttttgc
      541 cttttccgga tcgccatgaa acagagccag gcgcgctacc tgcacgtcac gcatcgcata
      601 taaaccttgt tcagagatac gctctgctgc tgccgttttc tgggtttgct taacttgctg
      661 agtttgctgt tgtgccgcta ccggtgcatt atcagcagcc catacagttg aagatgccag
      721 aattgctgtg accatcgtag ccataattaa acgtttcata cttacctcat tctttaacag
      781 ttttagaaaa cgccgccatc atattgggtg agcatgaaat gagaaatcag agagcaacgg
      841 acattttgaa agggttatct tacaactgta gttattatcg caaagccgct tttagtctta
      901 cagttgt
//
LOCUS       DADSZD010000383          892 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.385, whole genome shotgun sequence.
ACCESSION   DADSZD010000383 DADSZD010000000
VERSION     DADSZD010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.385"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..816
                     /locus_tag="J1Z28_004581"
     CDS             <1..816
                     /locus_tag="J1Z28_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBA7719317.1"
                     /translation="ALRESICAVIAGKDGYQADTLSLLPGVRIISRFGTGIDNIDLRA
                     AQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKR
                     IGLVGYGNIGKTLAKRLSGFDVELLAFDKQPDYQVADKAGVQFVSIEDIFMQSHVIIV
                     LLPFSSELENFISHKYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSS
                     EPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIHVAQSIIDYFAGREIKNVL"
BASE COUNT          243 a          170 c          226 g          253 t
ORIGIN      
        1 gcattacgag aatctatttg cgcggtcatc gccggaaaag atggttatca ggcggacacg
       61 ctatcattgc tgcctggtgt gagaataata tcgagattcg ggacggggat agacaatatt
      121 gatcttcgtg cggcacagca atccggtatt gttgtcaata atgcggttgg gattaattca
      181 aatgcggttg cggaatttat cattggactt atcttcgcga gcatgagaaa tatccctggc
      241 agctatcatg cgatgcaaaa tggctactgg ggtgagtcgc atggctgtga attacaaggg
      301 aaacggattg gcctggttgg ctacggtaat ataggtaaaa ctctggcgaa aaggttatcc
      361 ggttttgatg ttgagctttt agcttttgac aaacaacccg actatcaggt tgctgacaaa
      421 gctggagttc agtttgtatc aattgaagat atctttatgc agtcgcatgt catcattgtt
      481 cttttgcctt tttcttctga gctggagaac ttcattagtc ataaatattt gtcgatgatg
      541 cgcaatggtg ctctgatcat caatgccgca aggggaaagt tgctggatga gggcgcttta
      601 cttcaggtga ttgaagaacg aaatgtgttt gcggcgctgg atgtgtttag ctctgaaccg
      661 ttggcgcaat ttagcccact gctacatgcc aaaaatatca ttacaacgcc gcatatcgcg
      721 gccgccaccg ttgagtcata ccagcaaacc gggatacacg ttgcccagtc gataattgat
      781 tacttcgcag gaagggagat aaaaaacgtg ttgtgagttc agcgaacaaa gattttaaat
      841 acaaaaaaac cgcaatttgt agaacacatt gcggtttgtc agcattctaa ta
//
LOCUS       DADSZD010000384          880 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.386, whole genome shotgun sequence.
ACCESSION   DADSZD010000384 DADSZD010000000
VERSION     DADSZD010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.386"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            259..861
                     /locus_tag="J1Z28_004582"
     CDS             259..861
                     /locus_tag="J1Z28_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBA7719318.1"
                     /translation="MNTSPWNKDRIIGQKRPLQISHIWGIRIRLELEGKTRDLALFNM
                     ALDSKLRGCDLVKLKVSDVAYGSSVSSRATVLQQKTGSPVQFEITKGTREAVSALIKL
                     GNLRSKDYLFRSRVGTNQHISTRQYNRIFHGWVAKLGLEDSLYSTHSLRRTKPYLIYK
                     KTKNLRVIQLLLGHKKLESTVRYLGIEVDDALEISESIEV"
BASE COUNT          269 a          201 c          190 g          220 t
ORIGIN      
        1 gacccatgcg tacccgcagg atcttcgggt gcacgtgacc ataaagcagg tgcctaccaa
       61 agctgtggcg attcagatcc ctaaactgat ggggtcccaa aaagtatgcc atagcaagtg
      121 taagtatccc taaaatctgc actattctct ttttataaat cctctgacat gtgaattaga
      181 ttgccctaaa gtgagcttcc gcgcctcgct cataaggaca accatactca aatctcccac
      241 attgcaggag atttgagtat gaacacgtca ccgtggaaca aagaccgtat cataggccaa
      301 aaaagaccac ttcagatatc tcatatctgg ggtatccgaa tccgacttga actggaaggt
      361 aaaacacgcg atttagctct gttcaacatg gccttggaca gtaagcttcg aggctgtgat
      421 ctagtcaaac tcaaagtatc tgatgttgca tatggtagct ctgtttcaag cagagctacg
      481 gttctacaac agaaaaccgg cagccctgtc caatttgaga taaccaaagg gacaagagaa
      541 gctgtttctg cattgataaa gcttggcaat ttacgcagta aagactattt gttccgatct
      601 cgggtcggta ctaaccaaca catatcaacc cggcaataca accgaatttt tcatgggtgg
      661 gtagcaaaac ttggtctcga agattcgctt tacagtacac attccttgag aagaacaaaa
      721 ccttacctga tttacaagaa aactaagaat ctcagggtga tccaacttct gttaggccat
      781 aagaaactgg aaagcacagt ccgttatctg ggcattgaag tcgatgatgc gttagagatc
      841 tctgaatcga ttgaagtcta aggttgccag ggctgcaaca
//
LOCUS       DADSZD010000385          866 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.387, whole genome shotgun sequence.
ACCESSION   DADSZD010000385 DADSZD010000000
VERSION     DADSZD010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.387"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(74..352)
                     /locus_tag="J1Z28_004583"
     CDS             complement(74..352)
                     /locus_tag="J1Z28_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBA7719319.1"
                     /translation="MPVKVVRCFHDHTGWFNNIRRNILMITPVSLMDDQMVDMAFITQ
                     LTGLTDKWFYKLIKDGDFPAPIKMGRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          241 a          186 c          256 g          183 t
ORIGIN      
        1 acgggggata acaggcagca gaacgccgcc ggcaggccgg agactgcgtc tgctgcagat
       61 gaacggttaa gaattacgga cgggactgtg caatacgcgc ctgcagccag gcttccactt
      121 cgcttttcag ccatcgggag ctgcggccca ttttgatggg cgcaggaaag tcaccatcct
      181 tgatgagttt gtaaaaccac ttatcggtca ggccggtcag ttgagtgata aatgccatgt
      241 cgaccatctg gtcatccatc agtgaaactg gggtgatcat gagaatgttc ctccggatgt
      301 tgttaaacca gccggtgtgg tcatgaaaac aacgcaccac cttcaccggc ataacaaccg
      361 gggagaaacg taaaaaataa tcgggaatgt gaccgccagg agataaacgg ggaggttacg
      421 gtggctgagc cgtaccggta tgccgggtgg ttgttacgaa acgatgcttc cgcacgcgcg
      481 cgcgataaag aagggatgat tttcgtgagg aagaggggaa aactggcgtt ctgataattg
      541 cagagttatt tacggggcgg ttacgatggg gcagaccgtg tccggtgggc aggcgacagg
      601 tatcaccgat gagataagac tgtctgattg gtattataaa tatataagag agagcaaaca
      661 catgagatca aatacatgag ttcatatatg tgaaataaac acggtgagtg tgatcagtgg
      721 gcatgatgag aacgcagtag caacagactg cgctatccct gatagctggc gtataacctg
      781 cgtatataaa acgtaccagc aacgcacgtg aacagagtgc attaagcatc cgtcgttgac
      841 accgacctgg tgagtgacag tctgag
//
LOCUS       DADSZD010000386          851 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.388, whole genome shotgun sequence.
ACCESSION   DADSZD010000386 DADSZD010000000
VERSION     DADSZD010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.388"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..764
                     /gene="yeeO"
                     /locus_tag="J1Z28_004584"
     CDS             <1..764
                     /gene="yeeO"
                     /locus_tag="J1Z28_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBA7719320.1"
                     /translation="WVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVESVLF
                     TSGRLFTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVGQIAQ
                     AEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALFMPIW
                     SASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMFADWA
                     VRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
BASE COUNT          177 a          169 c          247 g          258 t
ORIGIN      
        1 tgtgggtgct ggcgattggt tttaatcctg cgctaaggat ttcgttaaag agctatttta
       61 aaccgctgaa ttttagcatt atctgggaag tcatggggat tggtattcca gcgagtgtcg
      121 aatcagtgtt atttaccagt ggtcggttat tcacccaaat gttcgttgcc gggatgggga
      181 ccagcgttat tgccggaaat tttatcgcgt tctcaattgc ggctcttatc aacttacccg
      241 gaagtgcgct cggttctgct tctacgatca ttacaggccg aaggttgggg gtagggcaga
      301 tagcgcaagc agagattcag ttgcggcatg tgttctggct gtccactctt ggattaacgg
      361 ccatcgcctg gctaacggct ccctttgccg gggttatggc atcgttttac acccaggatc
      421 cacaggttaa acacgtcgtt gtgattctga tttggctaaa tgctttattt atgcctattt
      481 ggtccgcctc atgggtgcta cccgctggat ttaaaggtgc tcgtgatgcc cgttacgcca
      541 tgtgggtttc gatgttgagc atgtggggtt gtcgggttgt agtcggttat gtgctgggaa
      601 tcatgcttgg ctggggtgtg gttggtgtct ggatgggaat gtttgccgac tgggctgtgc
      661 gggccgtgct gttttactgg cgaatggtta ctggacgttg gctatggaaa taccctcgat
      721 ccgagccgca aaagtgtgaa aaaaagccag ttgtgtcgga ataaacgaca aaatgcagat
      781 tatttcagca aacgatttca aatttaaaaa acaggctttg acattgtggg tgggcatcgc
      841 taatattcgc c
//
LOCUS       DADSZD010000387          846 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.389, whole genome shotgun sequence.
ACCESSION   DADSZD010000387 DADSZD010000000
VERSION     DADSZD010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.389"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(59..763)
                     /locus_tag="J1Z28_004585"
     CDS             complement(59..763)
                     /locus_tag="J1Z28_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7719321.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDATQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTDDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          224 a          201 c          169 g          252 t
ORIGIN      
        1 tattaaataa tttcaatctc aggttacaac tttttgtaac ctgaaatcgt atttatattt
       61 actcgtacag cacaacaaaa cttgctgttg acttaactac accaggtgca acagtgctac
      121 cgttttgagc ataacctgca gtaaaggccg cgctgaaatc atgggtcgta gcatttcctg
      181 gaattgtata agcggcggtg gaggcgctac cctctgccgc agtagtattc aggttaatca
      241 gaccattatt cgtcgtacct ttggagtaaa ttcgaatccc tacaccagtc gcccccgcat
      301 tgccgctgtt gtcagcatcc gttgatcctt ctacttcgtt agcgagcgtt ttattagcgc
      361 tatctgcagt accggtaaaa cggaactcaa ttttctgatc atctgtattg ctacattcgg
      421 tcagattaat attgaaatcg gtctggttgc tcgccccctg tgcttctacg tcgataccag
      481 tgaaaaggtt ggaaggcaca accggcagga caacttcaat ctgttttgaa tcggttgcga
      541 ccttacaggt agctttcact acacggccag taaagcggat tttccccccc attgaatcaa
      601 cgttgtcatc catatcagtg agcctgggat aatcaacggt gctccagtca gtatccgccc
      661 caccaaattg tgttgcatcg ccgggtgctg cggttaccgc agtagacatc agcatacctg
      721 atgccagtaa tactgcggat aaattagata aattgaattt catttatacc tttccttttg
      781 ttaaaaaatc caagcaacat tggtgttttt acattctgcg ttaataagtt atatctcaca
      841 aaaaaa
//
LOCUS       DADSZD010000388          844 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.390, whole genome shotgun sequence.
ACCESSION   DADSZD010000388 DADSZD010000000
VERSION     DADSZD010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.390"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            84..611
                     /locus_tag="J1Z28_004586"
     CDS             84..611
                     /locus_tag="J1Z28_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7719322.1"
                     /translation="MNKVIIATMLASTVSFGVNAANQGQGVINFKGTVIDAPCGIAQE
                     SADQTINFGQISKAHLNADGISVKKDLDIKLVNCDATEIGKLTNGVKVSFTGTTINGQ
                     NQELGTTGDTGTAIVVSAANGSLVSFDGTAGSATKVKEGDNTLRYSTWVKKATGGTLK
                     EGDFTAVANFNLAYE"
     gene            687..>844
                     /locus_tag="J1Z28_004587"
     CDS             687..>844
                     /locus_tag="J1Z28_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7719323.1"
                     /translation="MFWRMIISVPFFWCAMAQALPSASFPPPGMTLPEYWGEEHVWWD
                     GRASFKGQV"
BASE COUNT          234 a          160 c          207 g          243 t
ORIGIN      
        1 tgagacataa aagtactttt tgttgagtga cagaattttt gccactcaat aaaatgtatt
       61 ttttcaactc agggttttgt tatatgaata aagttattat tgcaaccatg cttgcttcta
      121 ctgtttcatt tggcgttaat gctgctaatc agggacaggg cgttattaac ttcaaaggta
      181 cagtaattga tgcaccttgt ggtattgcac aagaatcagc agatcagaca attaatttcg
      241 gccagatttc taaagcacat ttgaatgcag acggaatttc cgtaaaaaaa gatctggata
      301 tcaagttagt taactgcgat gccacagaaa tcggaaaact gaccaatggt gtgaaagttt
      361 cttttactgg tacaacaatc aatggacaga atcaggagtt aggtactact ggtgatacag
      421 ggactgcaat agttgtctca gctgcgaatg gcagcctggt ttcatttgat ggtacagcag
      481 gttccgcgac aaaagttaaa gaaggtgata ataccctgcg ctacagtact tgggttaaaa
      541 aagctactgg tggcaccctg aaagaaggcg attttaccgc tgttgctaac ttcaatctgg
      601 cctacgagta atctgtcggt cagttctgtc tgatatgttc gggggaattc cccgaacaat
      661 tgccagtgag aagtaaggaa taccgcatgt tctggcgcat gatcatttcg gtaccgtttt
      721 tctggtgtgc gatggctcag gccctgccat cagcttcttt tccaccaccg gggatgactt
      781 tgcccgagta ctggggagaa gaacatgtct ggtgggatgg gcgggccagc tttaaagggc
      841 aggt
//
LOCUS       DADSZD010000389          835 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.391, whole genome shotgun sequence.
ACCESSION   DADSZD010000389 DADSZD010000000
VERSION     DADSZD010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.391"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            93..>835
                     /locus_tag="J1Z28_004588"
     CDS             93..>835
                     /locus_tag="J1Z28_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HBA7719324.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDHHRNNSPL
                     PPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPTPPKPISFNNDVILDKTEKTLTIRD
                     SVFTYTENADGTISLQDSNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGEL
                     VITGDNTTVNNTGKTIVDGKGATGTEIAG"
BASE COUNT          256 a          193 c          184 g          202 t
ORIGIN      
        1 actggcaata ttggacgtcc tctatgtttt aaataatcaa ttggtcgggt cagggtatcc
       61 gaccttcttt atattcgcca gaaggattta ttatgcaaag gaaaactcta ttgtcggcct
      121 gtattgcatt agctctgagt ggtcagggtt gggcggcaga tattacagaa atagaaacca
      181 ccacaggtga aaagaaaaat accaatgtga cttgtccagc agatcctgga aaactcagtc
      241 cagaagagct taaacgctta ccctctgaat gctcctctgt agtcgaacag aacctgatgc
      301 catggcttgt cacaggtgct gctactgcgt taatcacaac cttagccata gtggaactaa
      361 acgacgatga tgatcatcat cgcaataatt ctccacttcc accaactcca cctgatgatg
      421 attcagacga cactccagtt cctccaactc ctggcggaga tgagataata ccagacgatg
      481 gtccggatga taccccgacg cctcccaaac cgatttcgtt taataatgac gttatcctcg
      541 ataaaacaga aaaaacgtta actattcgcg attcagtttt tacttatacc gagaatgctg
      601 acgggactat ttctctgcaa gatagcaatg ggcgtaaggc gactattaat ctttggcaga
      661 ttgatgaaac gaataacacg gttgcccttg aagggatgag cgcagatggt gcaacgaagt
      721 ggcaatataa tcacaacggt gagctcgtta ttacgggcga caataccaca gtaaacaaca
      781 ctggaaaaac catcgtcgac ggcaaaggcg ccaccggaac tgaaatcgcg ggtaa
//
LOCUS       DADSZD010000390          788 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.392, whole genome shotgun sequence.
ACCESSION   DADSZD010000390 DADSZD010000000
VERSION     DADSZD010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.392"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..555
                     /locus_tag="J1Z28_004589"
     CDS             <1..555
                     /locus_tag="J1Z28_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBA7719325.1"
                     /translation="EWVKLFTDKVTRGGKMKKWMLAICLMFINEICHATDCFDLAGRD
                     YKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNELARYGIKPEHLTT
                     DPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLAYASEVYRIYTGI
                     KSSKGIQVPTTKKSLPEINSVQNN"
BASE COUNT          245 a          175 c          186 g          182 t
ORIGIN      
        1 gagtgggtta aattatttac ggataaagtc accagaggtg gaaaaatgaa aaaatggatg
       61 ttagccatct gcctgatgtt tataaatgag atctgccatg ccactgattg ctttgatctt
      121 gcaggccggg attacaaaat agatcctgat ttactgagag caatatcatg gaaagaatcc
      181 cgttaccggg ttaatgccat cggtattaat ccggtaacgg gatatggcag cggactgatg
      241 caggtagatt cccagcattt taacgaactg gcccgctatg gaattaagcc ggaacatctg
      301 acaacagatc cctgcatgaa catttatacc ggtgcttatt atctggcaat agcctttaaa
      361 aaatggggcg tctcctggga ggccgttggt gcatacaatg ctggattcag gaagaccgaa
      421 cgccagaacc agagacgtct tgcctacgca tcagaggttt accggattta taccgggata
      481 aagagcagta aaggcatcca ggttccgacc acgaagaaat cacttccgga aattaacagc
      541 gtgcagaaca attaatctta cagacattgt cagataccgg ttatgccgca aaagcggcat
      601 ggaaggccac acacaaaaat atcatatgag tgacaatact gactggccga agccccgcga
      661 agcgggaacc catcttatat atacccggta actgcgcagc ctcctgccag accatttttc
      721 gcggtcacaa tatttacaga caaccggaaa tccggttgat gaaattgagg tatatcaaac
      781 gacacagc
//
LOCUS       DADSZD010000391          788 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.393, whole genome shotgun sequence.
ACCESSION   DADSZD010000391 DADSZD010000000
VERSION     DADSZD010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.393"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..696)
                     /locus_tag="J1Z28_004590"
     CDS             complement(<1..696)
                     /locus_tag="J1Z28_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HBA7719326.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAERFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGV"
BASE COUNT          226 a          175 c          147 g          240 t
ORIGIN      
        1 tacgccctca ataatctggc gtgaattaag ccccaggctt tcagcataac tatcaagttc
       61 attgaaataa gccacacgca tagccagata agtgttggca aaaagtttaa tcgcctcagc
      121 ttcggttgag tcagtaaaca atgttggtat gtcttgctta atggcgcctt cctgtaataa
      181 cgcagcaaaa cgttcagcgc gttcagactg ctcgccaatc acaatgcgtg atgggtgtaa
      241 gttatcataa agtgctttac cttcacgcaa aaactcaggc gaaaagatca cattttcaat
      301 accaaaacgt tctttaatgg actctgtaaa accaacaggg atagttgatt ttataatcat
      361 taccgcgttg ggattaattt ctgtcacatc gcgaatgacc gcttccacgc ttgaggtatt
      421 aaaataattc gttttcggat cataatcggt aggtgtggca ataataacat aatcggcatt
      481 tttatacgcg tcatacttat ctgtcgtagc gcggaaattg agatctttag tcgccagata
      541 ctcttcaatc tccttatcaa caatcggtga ctgcttcttg ttaagcatgt ccactttggc
      601 ctgaacgata tccagtgcaa ccacttcgtg gttttgcgca atcagaatac catttgaaag
      661 accaacataa cctgttcctg aaattgttat tttcattagc tctgacttct tccggttaaa
      721 catttagagt ggtcattaat ccaccaccac aacttcatct actgcgggat ttttaacgct
      781 gatgtata
//
LOCUS       DADSZD010000392          785 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.394, whole genome shotgun sequence.
ACCESSION   DADSZD010000392 DADSZD010000000
VERSION     DADSZD010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.394"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            33..347
                     /locus_tag="J1Z28_004591"
     CDS             33..347
                     /locus_tag="J1Z28_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005057500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBA7719327.1"
                     /translation="MTIDECATWIAQTGDSESWRQWENGKCAIPDCVVEQLLAMRQQR
                     KKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIYQSVAAELYAHDLER
                     LC"
     gene            447..>785
                     /locus_tag="J1Z28_004592"
     CDS             447..>785
                     /locus_tag="J1Z28_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7719328.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLD"
BASE COUNT          198 a          180 c          172 g          235 t
ORIGIN      
        1 atgaactcca ggcacttcgc catatttttg ccatgactat tgatgaatgc gcgacctgga
       61 ttgcccagac cggtgacagt gaaagctggc gtcagtggga aaatggcaaa tgcgccattc
      121 ctgattgtgt agtcgagcaa ctgttggcta tgcgtcagca acgaaaaaaa catcttcatg
      181 ccatcataga gaaaataaat aaccgcatcg gcaacaacac catgcgtttt ttccccgact
      241 taaccgcgtt tcaacaagtc taccctgatg gcaattttat tgactggaag atctatcaat
      301 cggtagccgc tgaattatat gcacacgatc tggagcggct ttgttaattt ctccacagaa
      361 aggaattgtc gttgttacaa caataatgaa cggatactga cacaacatcg ctacactttt
      421 taaagcacct ttgctaagta gaacctatga aaaatcccta ttaccctacc gcacttgggt
      481 tgtattttaa ttacctggtg catggtatgg gcgtcatttt gatgagcctg aatatggcct
      541 cgctggagac gctttggcag actaatgccg cgggcgtctc gatagttatc tcatcactgg
      601 gcattggtcg attaagtgtc ttgctttttg caggattatt atccgatcgc tttggtcgcc
      661 gcccttttat catgctcggg atgtgctgct atatggcctt cttttttggc atcctgcaca
      721 ccaataacat cattatcgct tatgtttttg gctttctggc gggaatggca aacagttttc
      781 tcgat
//
LOCUS       DADSZD010000393          782 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.395, whole genome shotgun sequence.
ACCESSION   DADSZD010000393 DADSZD010000000
VERSION     DADSZD010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.395"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>782
                     /locus_tag="J1Z28_004593"
     CDS             <1..>782
                     /locus_tag="J1Z28_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBA7719329.1"
                     /translation="DLNLLVIFECIYQHLSISKAAESLYITPSAVSQSLQRLRAQFND
                     PLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFIIYGPQLISCTN
                     NNMLIRCLRQDTSIDIECHDILLSAENAEELLVQRKTDLAITLQPVISRSVICMPLHT
                     IRNSLICSNKHPRITDTSNYEQIIAEEFTLLISKSAGIDEIQMDIDERFMNRKVSFRS
                     SSLMTIINSISVTDLLGIVPTELYDLHRDFLKLKEIKLEQSL"
BASE COUNT          249 a          165 c          129 g          239 t
ORIGIN      
        1 ttgacctcaa tttactggtc atatttgaat gtatttatca acatctcagc attagtaaag
       61 cggcagaatc attatatata acgccctctg ctgttagtca gtcgttacaa cgtttacgtg
      121 cgcaatttaa tgacccgtta ttcattcgct cagggaaagg tatcgcgccg actacaacag
      181 ggttaaacct gcatcaccat cttgagaaaa atctcagagg ccttgaacaa acgatcaata
      241 tcgttaataa gtctgaacta aagaaaaatt ttattatcta cggtcctcag cttatttctt
      301 gtactaataa caacatgcta atccgctgtc ttagacaaga tacttcaatc gatatagaat
      361 gccatgacat actgctttca gctgaaaatg ccgaagaact gttggttcaa cgcaaaactg
      421 acctggcaat tacgctgcaa ccagtaatta gtcgctctgt catctgcatg ccgttacaca
      481 ctattcgcaa ttcccttata tgtagcaaca aacatcctcg tattactgat accagtaact
      541 atgaacaaat tatcgccgaa gaattcaccc tgttgatatc taaatcagca gggattgacg
      601 aaattcaaat ggacatagat gaacgtttta tgaaccgtaa agtttcattt cgcagttctt
      661 ctttgatgac tataattaat agtatttctg ttactgactt attaggcatt gtgcccaccg
      721 aactgtacga tttacatcgt gactttttga agttaaaaga aataaaactg gaacaatctc
      781 tt
//
LOCUS       DADSZD010000394          767 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.396, whole genome shotgun sequence.
ACCESSION   DADSZD010000394 DADSZD010000000
VERSION     DADSZD010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.396"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            25..360
                     /locus_tag="J1Z28_004594"
     CDS             25..360
                     /locus_tag="J1Z28_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7719330.1"
                     /translation="MAELGRRITALRKDAGMTQTQVAQALNVSQQAVQAWEAGRRRIQ
                     ISILPAVARLLAVSLEDLLGEEPEKIARKRGPAPKWQQLIEEIDSLPKAKQKMISEML
                     SALIVQARN"
     gene            439..>767
                     /locus_tag="J1Z28_004595"
     CDS             439..>767
                     /locus_tag="J1Z28_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBA7719331.1"
                     /translation="MAEHDITPEVTISKTQRRLKVGYVGISHTNRKTKVPTGYSRSPS
                     LHLKGNWLAEAGFDTGRGVTVKISEGCLTIIADSDEMQELREELYQVKQAVKGMRDGM
                     FSALNES"
BASE COUNT          210 a          188 c          225 g          144 t
ORIGIN      
        1 ataagcaacg aagaacaggt atttattgcc gaacttggca gacggatcac cgcgctgagg
       61 aaggatgccg ggatgacaca gacgcaggtt gcacaggcgc tgaacgtctc ccagcaggct
      121 gtacaggcat gggaagccgg acggcggcgg atacagatct ccatactccc agccgtggca
      181 agactgttag cggtttctct ggaggatttg cttggcgaag aaccggagaa gatcgcccgc
      241 aagcgtggcc ccgcgccaaa atggcagcag cttattgaag agatcgacag cctgccgaag
      301 gcaaagcaga agatgatttc ggagatgtta agtgcgctga ttgtgcaggc acgtaactga
      361 ctgataaata acgcggtgat taagggcggc aacccttaac caccgctcac cacaagcaac
      421 taatcgaggt agttaatcat ggctgaacac gatattacgc cagaagtcac catttccaaa
      481 acacagcgcc gtcttaaagt cggctatgtc ggtatctccc acaccaaccg taaaaccaaa
      541 gttcccaccg gatattcgcg cagccccagc ctgcacctta agggcaactg gctggcagag
      601 gcgggatttg ataccgggcg gggtgtcaca gtgaagattt cggaggggtg cctgaccatc
      661 atcgctgaca gtgacgagat gcaggaactg cgggaagaac tttatcaggt taaacaggcg
      721 gttaagggga tgcgtgacgg gatgtttagt gcgctgaacg aaagtta
//
LOCUS       DADSZD010000395          762 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.397, whole genome shotgun sequence.
ACCESSION   DADSZD010000395 DADSZD010000000
VERSION     DADSZD010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.397"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..221)
                     /locus_tag="J1Z28_004596"
     CDS             complement(<1..221)
                     /locus_tag="J1Z28_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719332.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFRDANVYPLDQAFPLLMKQL"
     gene            complement(384..605)
                     /locus_tag="J1Z28_004597"
     CDS             complement(384..605)
                     /locus_tag="J1Z28_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBA7719333.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(674..>762)
                     /locus_tag="J1Z28_004598"
     CDS             complement(674..>762)
                     /locus_tag="J1Z28_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBA7719334.1"
                     /translation="LGLVDIRVPDHLIVGGNQVFSFAEHGLL"
BASE COUNT          185 a          193 c          223 g          161 t
ORIGIN      
        1 tccagttgtt tcatcagcag cggaaaggcc tggtccagag ggtacacatt cgcatctctg
       61 aaccggcctc tgataccggc acggtcggca aggtaatgca accggttacc ctcctgcacc
      121 agacgggcgc cgaaacaggg cgtcactgtg cagggtagtc cccaccaggg atggtcgtga
      181 ttgtcatcgg gatgcgttgt cccggagagt gtgtctgaca cgataaaatc ctcatagaaa
      241 atcggtgaat gatagtttaa cgatgacggg tcagccggcg cgcagaccgg cttcggtaaa
      301 ggtgtattcg ttgatgagca ggacctcatc cacagcggcg tctgagctca gccagtcgta
      361 ctcgttttcc gggtgacgga acatcagttc agggtgacgc tcatcagccg gacatacggg
      421 ccaaaactgt ccttacggcg ttctgcaaat acagccagca caccgggaat atcctgcact
      481 tcacgaccgg tatacgcttc agcactgccg tgccagcggt atttgccggt acagaacgga
      541 aaaagacggg atgtcggatg ttgttggtga atacgcatgg cttcaccacg ggtgatgatt
      601 ttcatagtgt gatacctctg aagacagaag ataaaagtga aaacaggtgt gatgtgattg
      661 tggcggtgac gggttaaagc aggccgtgtt ccgcaaagga gaaaacctgg ttgccaccga
      721 ctatcagatg gtccggcacc cggatatcca ccaggcccag tg
//
LOCUS       DADSZD010000396          756 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.398, whole genome shotgun sequence.
ACCESSION   DADSZD010000396 DADSZD010000000
VERSION     DADSZD010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.398"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>756
                     /locus_tag="J1Z28_004599"
     CDS             <1..>756
                     /locus_tag="J1Z28_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7719335.1"
                     /translation="VFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPGQNLVVDLSQHIS
                     CWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNVLDIGDKTP
                     MPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNIISNNSVVMP
                     TGGCTVDSRNVTVNLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSARQVFANTAPD
                     ATKASGVGVSLMRNGKTLATGENVSLGTVNKSK"
BASE COUNT          215 a          162 c          177 g          202 t
ORIGIN      
        1 aagtttttgc cttcagttgt aatgtcgatg gcgggtcaag tattggagca ggtacaacct
       61 ctgtttatgt taatctcgat cctgtaatac aaccgggcca gaatctggtt gtagacttgt
      121 ctcagcatat aagttgctgg aatgattacg gcggctggta cgacactgat catataaacc
      181 tggtacaagg atcagctttc gcaggctccc tacagtcata taaaggatca ctgtactgga
      241 ataatgtgac ctaccctttc ccgttgacga caaataccaa cgtattggat attggtgata
      301 agacaccgat gccattaccg ttaaaactct atattacccc agtcggagcg gctggtggag
      361 ttgtcataaa agcaggggaa gttattgccc gtattcatat gtataaaatt gccacgttag
      421 gaagcggaaa tccgcgtaat tttacgtgga atattatttc aaataacagt gttgttatgc
      481 caacaggcgg ttgcaccgtt gatagtcgta atgtcaccgt gaatctcccg gactttcctg
      541 gaagcgcaga aatacccctt ggtgtatatt gctctagcga acaaaagctc tcattttatc
      601 tttcgggtgc caccactgac agtgcccgac aagtgtttgc taacacagca ccagatgcaa
      661 caaaagcatc gggcgtgggt gtctctctga tgcgtaatgg aaaaacactg gcaacaggag
      721 aaaatgtctc cctggggaca gtaaataaaa gcaaag
//
LOCUS       DADSZD010000397          740 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.399, whole genome shotgun sequence.
ACCESSION   DADSZD010000397 DADSZD010000000
VERSION     DADSZD010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.399"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>740
                     /locus_tag="J1Z28_004600"
     CDS             <1..>740
                     /locus_tag="J1Z28_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBA7719336.1"
                     /translation="DYLWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYDHDHY
                     YYDKAGNLLADEYQSAVMDNRLPGYGRDRYRYNEWGELKERRDQQLEWNAQGQLTRVI
                     SSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRTYLYD
                     AEQPYTPVAAITGRGESRKIWYYHTDLTGTVQEVTAADGTLVWAGYQAGFGENRGDIS
                     NSGAYFEQPLRLPGQYYDEETGLHYNLF"
BASE COUNT          182 a          182 c          259 g          117 t
ORIGIN      
        1 gattacctct ggaacgcaga cggtgaagtc ggcggcatca atgacaaact gcgcggttgc
       61 ctggtgttca gctatgaccg gagcggctgg ctgaccagcc gcacgggtca gatgtacgac
      121 catgaccatt attactacga taaagcggga aacctgctgg cggatgagta ccagagtgcg
      181 gtgatggaca accgcctgcc gggctacggg cgtgaccgtt accgttataa cgaatggggc
      241 gaactgaaag agcgcaggga ccagcagctg gagtggaacg cgcagggcca gttaacgcgg
      301 gtcatcagca gcaacagcga aacgcggtat cagtatgatg cactgggcag gcgcatcagc
      361 aaggcgacca gcaatctcca tacggaccgg ggagaacgaa gccggacaac gtttgtgtgg
      421 gaagggttcc ggctgttgca ggagacaacg tggcagggaa agcggacata tctgtacgac
      481 gcggaacagc cgtacacccc ggtggcggcc atcacggggc gcggagaaag ccggaaaatc
      541 tggtattacc acacggacct gacgggcacg gtacaggagg tgaccgcggc ggacgggacg
      601 ctggtgtggg caggctacca ggcgggattt ggtgaaaacc ggggcgacat cagcaacagt
      661 ggggcgtatt ttgagcagcc gctgcgactg ccggggcagt attatgatga agagacgggg
      721 ctgcactata atctgttcag
//
LOCUS       DADSZD010000398          731 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.400, whole genome shotgun sequence.
ACCESSION   DADSZD010000398 DADSZD010000000
VERSION     DADSZD010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.400"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            189..>731
                     /locus_tag="J1Z28_004601"
     CDS             189..>731
                     /locus_tag="J1Z28_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid:lipid A palmitoyltransferase"
                     /protein_id="HBA7719337.1"
                     /translation="MNVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     ATFQMTYIPGTYNNGNVYFAW"
BASE COUNT          193 a          140 c          181 g          217 t
ORIGIN      
        1 atgactattc ccatcgcaga aaacgatgcg tcgtctttaa tcgatgcgcg gaaatattta
       61 tcttgaacta gcggaaataa atagggcagc tattcatatt attcttcatg ttggggctat
      121 tgaggttatg ttaattgtag ctttgctatg ttagtagtag atttttgata aatgtttaat
      181 ggtcacaaat gaacgtgagt aaatatgtcg ctatattttt ctttgttttt attcagttaa
      241 tcagtgttgg taaagttttt gctaacgctg atgagtggat gacaacgttt agagaaaata
      301 ttgcacaaac ctggcaacag ccagaacatt atgatttata tattcctgcc atcacctggc
      361 atgcacgttt tgcttacgac aaagaaaaaa ccgatcgcta taacgagcga ccgtggggcg
      421 gcggttttgg tcagtcgcgt tgggatgaaa aaggaaactg gcatggcctg tatgccatgg
      481 catttaagga ctcgtggaac aaatgggaac cgattgccgg atacggatgg gaaagtacct
      541 ggcgaccgct ggcggatgaa aattttcatt taggcctggg atttaccgct ggcgtaacgg
      601 cgcgcgataa ctggaattac atccctcttc cggttctact gccattggcc tctgtggggt
      661 atggccccgc gacgtttcag atgacctaca ttccgggtac ctacaacaat ggcaatgtgt
      721 actttgcctg g
//
LOCUS       DADSZD010000399          728 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.401, whole genome shotgun sequence.
ACCESSION   DADSZD010000399 DADSZD010000000
VERSION     DADSZD010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.401"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            58..>728
                     /locus_tag="J1Z28_004602"
     CDS             58..>728
                     /locus_tag="J1Z28_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBA7719338.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALE"
BASE COUNT          244 a          125 c          158 g          201 t
ORIGIN      
        1 taacacatgt taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg
       61 ggacgtaaat gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa
      121 atttatgcaa aattcggtgt agaaatctat gctgctgcca aacaaggtga acccgatcca
      181 gaattaaaca catctttaaa attcgttatt gaacgtgcaa aacaagcaca agttccaaag
      241 cacgttattg ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtgcag
      301 ggacgttatg aaggctttgg tcctaatggc tcaatgatta tcgctgagac attaacttca
      361 aatgttaacc gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc
      421 ggagcggcag gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga
      481 acagaccctg accatatttt tgaaattttg cttgaagctg aagttgatgt tcgtgatgta
      541 actgaagaag aaggtaatat tgttatttat actgaaccta ctgaccttca taaaggaatc
      601 gcggcgctaa aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct
      661 caatctgaag ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc
      721 cttgaaga
//
LOCUS       DADSZD010000400          715 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.402, whole genome shotgun sequence.
ACCESSION   DADSZD010000400 DADSZD010000000
VERSION     DADSZD010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.402"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            122..712
                     /locus_tag="J1Z28_004603"
     CDS             122..712
                     /locus_tag="J1Z28_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7719339.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNIALHNIEGSTIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
BASE COUNT          216 a          150 c          143 g          206 t
ORIGIN      
        1 cttagtaaaa aacaaacaaa catcattaac aaagtaacgt tcagttacat ggcattgcat
       61 caaaatctca tcatcatgca atgctgttat ctgctttatg gaatattact caaggatttt
      121 tatgtctaaa aaattaggtt ttgcgctgtc cggtattatg ttggcaatgg ctgctggtac
      181 tgcatttgct gctaacgata tggacggcgg tcaattaaat attagcggtc tggtcgttga
      241 taacacttgt gaagctcgtg ttgatggcgg taacaaagac ggtctgatcc tgctgcaaac
      301 cgctactgtt gctgaaatta ctgatggtgt actggatacc actgtaggtg ctaaagctaa
      361 accattcagc attactattg actgcagcaa agctaacccg gctccgggta ctactgcaaa
      421 aatgaccttc ggttctgttt tctttggtaa cagcaaagga acactgaaca acgacatgtc
      481 tattaacacc ccgtctgacg gcgtaaatat cgctctgcac aatattgaag gttctactat
      541 taaacaggtt caggtcaata atccgggtga tgtttacagc aaaactctgg attcaacgtc
      601 taaatctgca acctacgatt ttaaagcatc ctatgttcgt gcggatgcaa gcaaagcagc
      661 tactgccggt tatgtaaaaa ccaactcagc atataccatt acttaccagt aaata
//
LOCUS       DADSZD010000401          698 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.403, whole genome shotgun sequence.
ACCESSION   DADSZD010000401 DADSZD010000000
VERSION     DADSZD010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.403"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(68..667)
                     /locus_tag="J1Z28_004604"
     CDS             complement(68..667)
                     /locus_tag="J1Z28_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBA7719340.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDNNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHEVLTGSDDGRRSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT          172 a          214 c          169 g          143 t
ORIGIN      
        1 catattcccc ccacaaaaaa gcccgccaga accggcgggc tgtcataaca ctgtgttacc
       61 tggctaatca gaacttataa ccgacaccca cgatgaaacc gtcagtgcgc cagtcgccac
      121 tgccggagcc ttcataagca atatcaatgg ccacggattc ggtcgggtta aactgcacgc
      181 cagcccccca cgccagagac gtgttgctgc ggcgaccgtc atcacttccg gtcagaactt
      241 cgtgcgtttt ccccttgttg tcagttacgc ggagataatc cccggagaaa gtcgacacac
      301 ggctgtaagc cacacccgcc atcgcatacg cgctgaacca ttcattcacg cgtacagacg
      361 gccccgccat cacgctgaac cagcggttac gcacggaatc ttcatgccag cgggtatcgc
      421 tgtaacgggt cagctggcga ttattgtctc ctgcatagct gaatgacgtc accagcccca
      481 gtgtgtccgt aaactcataa cggtatttca cgttaatccc gttaagatca tcgctgccgg
      541 gaacgttcgt cgaggcatga agatagcccg cgctcagcgt ggactgatgt tcagacgccc
      601 atgcaggcgc accggatacg gccagacaga tggctgcgga caaaatggcg gcataaagtt
      661 tacgcataat tacctctcgc ttttctgcaa taaaaaag
//
LOCUS       DADSZD010000402          693 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.404, whole genome shotgun sequence.
ACCESSION   DADSZD010000402 DADSZD010000000
VERSION     DADSZD010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.404"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(1..434)
                     /locus_tag="J1Z28_004605"
                     /pseudo
     CDS             complement(1..434)
                     /locus_tag="J1Z28_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            406..621
                     /locus_tag="J1Z28_004606"
     CDS             406..621
                     /locus_tag="J1Z28_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I addiction module toxin, SymE family"
                     /protein_id="HBA7719341.1"
                     /translation="MMTGNPPAFSTKWLREAGFEIGTGVTVKISEGCLILLADNNEVQ
                     ELRRELYQFKQAVKGIRDGMFSVLNKS"
BASE COUNT          142 a          184 c          203 g          164 t
ORIGIN      
        1 cggtgcaggc tgtcacgttc atattcgctg accgtgatac cgtccagtgc cgtctggagc
       61 agatgtccgc tgccgtagta aagatggctg aggtgctgcc cgtccggcag ggtaatgccg
      121 gtgggattgc cctgcgcgtc gcgctgatat tttatactgc cgtgcacgct gtgctctgcc
      181 gtcagccacc cgctgcggtc atacgcaaaa cgcaccctgt ccgcctgcat gtggtgcagc
      241 cgttcccctt cttccgtcgg ggtgagcgtc acttccgtta tctggtcaag gcggttgtag
      301 ctgaaagtgc gcgtactgtg cagcgtgtca ctgcgcaaca ggcggcccag cgcgtcacgc
      361 tcctgccgct cattacgcac cccgccttct gtatcctgcg tctggatgat gaccggaaac
      421 ccgcccgcat tcagcaccaa atggctgaga gaagcgggat ttgagatcgg cacgggcgtt
      481 accgtgaaga tctcggaagg ttgtctgatc ctgcttgcgg ataacaacga agtacaggaa
      541 ctgcgcaggg aactttatca gtttaaacag gcggttaagg ggatacgtga cgggatgttt
      601 agtgtgctga acaaaagtta aataataaaa aagccggggg ggattttatt tcctatccgg
      661 cttctaacat taatgcttca aggggaatga ctt
//
LOCUS       DADSZD010000403          691 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.405, whole genome shotgun sequence.
ACCESSION   DADSZD010000403 DADSZD010000000
VERSION     DADSZD010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.405"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            13..>691
                     /locus_tag="J1Z28_004607"
     CDS             13..>691
                     /locus_tag="J1Z28_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBA7719342.1"
                     /translation="MAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYVR
                     LGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRN
                     YGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQ
                     GKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQVIYGNNSLNASGQNAE
                     VWAAGLKY"
BASE COUNT          190 a          151 c          169 g          181 t
ORIGIN      
        1 gcatcagtac tgatggcgat gtctgctcag gcagcagaga tttacaataa agacagtaac
       61 aagctggatc tgtacggaaa agttaatgct aagcactact tctcttctaa cgatgcagat
      121 gatggcgaca caacttatgt tcgtctgggc ttcaaaggcg aaactcaaat caacgatcaa
      181 ctgactggtt tcggtcagtg ggaatatgaa ttcaaaggca accgtgctga atctcaaggt
      241 tcttccaaag ataaaacccg tcttgccttc gctggcctga aattcggtga ctacggctcc
      301 atcgattatg gccgtaacta cggtgtagca tacgatatcg gtgcatggac cgacgttctg
      361 ccagaattcg gtggcgatac ctggacccaa acagatgtgt tcatgactgg tcgcaccaca
      421 ggtgttgcaa cctatcgtaa caacgacttc tttggtctgg ttgatggtct gaactttgct
      481 gctcagtatc agggtaaaaa tgaccgcact gacgtaactg aagctaacgg tgatggtttc
      541 ggtttctcca ctacttatga gtatgaagga ttcggtgtag gtgcaaccta tgctaaatct
      601 gaccgcacta atgatcaggt tatctacggt aacaacagcc tgaatgcatc tggtcaaaat
      661 gctgaagtat gggcagctgg tctgaaatat g
//
LOCUS       DADSZD010000404          682 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.406, whole genome shotgun sequence.
ACCESSION   DADSZD010000404 DADSZD010000000
VERSION     DADSZD010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.406"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..333
                     /locus_tag="J1Z28_004608"
     CDS             <1..333
                     /locus_tag="J1Z28_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBA7719343.1"
                     /translation="EEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTP
                     RRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMA
                     ETMLESLR"
     gene            415..>682
                     /locus_tag="J1Z28_004609"
     CDS             415..>682
                     /locus_tag="J1Z28_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBA7719344.1"
                     /translation="MKSVSQNTPTIYSATTPENNPPQFVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDD"
BASE COUNT          163 a          185 c          181 g          153 t
ORIGIN      
        1 gaggagaagc gtgatgccat gcagtcgctg gtcctgccgc caccggcacg ccaggcgctg
       61 gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac caccgccgac
      121 attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtgc ttatcagacc
      181 atccaggaga atatgctgaa aggcgggatt tccggtcgca gtgcaaaagg aaaacgtatc
      241 catacccggg ccattcacag cattgatacc gacattaagc tcaatcgcgc attgtgggtg
      301 atggcagaaa cgatgctgga gagcctgcgc tgatgccgtt tccctgagtg agggaatcat
      361 tcagctttcg ttcctgaaaa acacacattt atttgttatt taaggagtta tctcatgaaa
      421 agcgtttctc agaatacccc cacaatttat tcagctacaa caccagagaa taatccgcct
      481 cagtttgttg ccagcctcgt ccctgatgaa cagcgcatca gcttctggcc gcagcatttt
      541 ggcctcattc cacagtgggt gaccctggag ccccgtgtct tcggctggat ggaccgtctt
      601 tgcgaagact actgcggtgg tatctggaat ctgtataccc tgaacaacgg cggggcattt
      661 atggcacccg aaccggatga cg
//
LOCUS       DADSZD010000405          679 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.407, whole genome shotgun sequence.
ACCESSION   DADSZD010000405 DADSZD010000000
VERSION     DADSZD010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.407"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..559
                     /locus_tag="J1Z28_004610"
     CDS             <1..559
                     /locus_tag="J1Z28_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBA7719345.1"
                     /translation="IPETVTMIGGNLAHSGPGFGNEDDMLFMTLEFPSGKLATLEWGS
                     AFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQEEDDDRRKGNMTSE
                     MDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDLLNGEAAMSAIATA
                     DAATLSRSQDRKVKISEIIKHTSVM"
BASE COUNT          225 a          122 c          145 g          187 t
ORIGIN      
        1 aataccagag acggttacta tgattggtgg aaatttggcc cattctggtc caggatttgg
       61 caatgaagat gatatgttat ttatgacctt ggaattcccg tcaggaaaac tagcaacctt
      121 agagtggggg agtgcattta actggccgga acattatgtc atcatcaatg gaactaaagg
      181 ctctattaaa attgatatgc aagaaacagc agggtcactt aggattggcg gtcagacaaa
      241 gcattttttg gtccatgaaa cacaagaaga agatgatgat cgtcggaaag gcaatatgac
      301 ctcagaaatg gatggcgcta tagcatatgg tcatccagga aaaaaaacac cattatggct
      361 tgccagttta ataagaaagg aaacgttatt cctccataat atcctctgtg gtgcaaaacc
      421 tgaagaagat tatattgacc ttctcaatgg tgaggcggcc atgtcggcga ttgctactgc
      481 tgatgctgcc actctttcaa gatcgcagga caggaaagtg aaaatcagtg agatcattaa
      541 acatacatca gtaatgtaaa aactatacgc ctcttgattt aattcaggag gccttttatg
      601 tatgatcaag aattttatct tcgataatct cataatttta gcagtgccat ttatgataaa
      661 aacatccctt aaaaccaat
//
LOCUS       DADSZD010000406          676 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.408, whole genome shotgun sequence.
ACCESSION   DADSZD010000406 DADSZD010000000
VERSION     DADSZD010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.408"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(235..468)
                     /locus_tag="J1Z28_004611"
     CDS             complement(235..468)
                     /locus_tag="J1Z28_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HBA7719346.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVLKIMQKVWEDDCWLH"
     gene            complement(479..667)
                     /locus_tag="J1Z28_004612"
     CDS             complement(479..667)
                     /locus_tag="J1Z28_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719347.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
BASE COUNT          180 a          175 c          118 g          203 t
ORIGIN      
        1 ccctgcaaac ttttcgcaaa cgaaagaata tccatcttat gatcgtcgag agctttgcct
       61 tgatacacaa tacttactga ttgagtctta tccgccattg agttcagatc cgttgccaat
      121 aaaatgcgcg cgattttacg cccatcacta tgtttaatct atctattaat catttatccc
      181 tctagctgtc tactatcgga ctcaaaaaac atgactcatt acaaacgaaa cctatcaatg
      241 cagccagcag tcgtcttccc acaccttctg cataattttc agcacttgtt ttctttcttc
      301 gtccagttgc agtccggtca gttccacacc gttagagcta cctttgcgga tacgaattac
      361 cgttttggga tacagggggc gcagattgcg gtaaagctcg gattcaaggg catctagggt
      421 agactggcta atcttctgct ctttatcgat cattatttca atgcgcataa aagtcacctc
      481 agctgatgac atccattgag cggttgtatt cgtgagttct gatttttgcc atgagttcat
      541 ctgtcagttc agaaacccac tgcaaagcca gccccttctc ttcatcacta cactcactag
      601 ccgctacaag tttaagaaaa aaatcaatgc gctggagctt caaagactcc aaaaaatagt
      661 cctgcatctt tcctcc
//
LOCUS       DADSZD010000407          670 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.409, whole genome shotgun sequence.
ACCESSION   DADSZD010000407 DADSZD010000000
VERSION     DADSZD010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.409"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            121..327
                     /locus_tag="J1Z28_004613"
     CDS             121..327
                     /locus_tag="J1Z28_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBA7719348.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          187 a          145 c          141 g          197 t
ORIGIN      
        1 caaaaataag tggtcggatg agtagtaaaa ttacacaaac agcggtacta ttttcatcag
       61 ataaacgaat tcatttttcc ggtgctattc accgggtaga tacaaaaggg ggagaaaagt
      121 atggaactct acaaagaata tcctgcatgg cttatctttt tacgccgtac ttgtgcggtt
      181 gcagcgggcg ttctggcgct gcctttcatg cttttctgga aagaccgcgc ccgcttttat
      241 agctacctgc accgcgtctg gtcgaaaacc agcgataaac cggtgtggat ggatcaggcc
      301 gaaaaggcaa ccggtgattt ttattgattg cctgcgcgct taagccagcc tcgtatcagg
      361 gggctggttt aagtgttcaa catcaacgtc ttccatgata attaacatgg aagacgtttc
      421 catcgtgtta ttcatttcat cactgtttta gcatcaagtt tcaatagtat cctttcatag
      481 gactactctg gatgcgttta ccttcattgt cgctctaagc aacaaatctc cagttaggtt
      541 gcatccttcc ttacacactt tcctgaaact tcagggtaag gtgagaaagc ttttctaatt
      601 ccagcatcta aataaattcc gaattttgct attaatatcg ctaccgcaat aaaaacagag
      661 agtaaagaga
//
LOCUS       DADSZD010000408          663 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.410, whole genome shotgun sequence.
ACCESSION   DADSZD010000408 DADSZD010000000
VERSION     DADSZD010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.410"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(55..366)
                     /locus_tag="J1Z28_004614"
     CDS             complement(55..366)
                     /locus_tag="J1Z28_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBA7719349.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     L"
     gene            complement(371..586)
                     /gene="essD"
                     /locus_tag="J1Z28_004615"
     CDS             complement(371..586)
                     /gene="essD"
                     /locus_tag="J1Z28_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBA7719350.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT          173 a          173 c          132 g          185 t
ORIGIN      
        1 cagcaccaat cagcgccaga acggcagccg acaggccgta tctgattttt gtgttcatag
       61 atatttatga tgaggacgct cgtgcttatt ggcaggattt tcaatcttaa aggagtactg
      121 atgctgcaga taagactcaa ctttttctga caatttttct gctacttcca ggaagacttg
      181 ccggacgctc cttctggctg ctgcctcata aaactccagc gcagctcctt caacacggtc
      241 catggcgaca tccaggtcaa aaatttcacc gtcaaagcgt tctttgtcct gtaaggctac
      301 agttaccgta actttattct caaaattacg gactcctttc acaaccagtt catagtcttg
      361 agtcattgga ttactctcct ctcgcagcct tacgcctgtc ttctttaatc ttgaaataaa
      421 gatttgtcag atacgtcagc aggccaaaaa ccaggctacc cagcacaccg attgcagccc
      481 actgtgacgg agttacttta tcgagtaact gcaatgccca gaaaccagca ttacccgccg
      541 atgtgccata ggcaacacct gttgttaact tatccattga tttcatatcc tcaccccgat
      601 gtacacggat ggtgcaatat gtttgaaaag atcggagtct acggggtagt tttgacagca
      661 cac
//
LOCUS       DADSZD010000409          662 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.411, whole genome shotgun sequence.
ACCESSION   DADSZD010000409 DADSZD010000000
VERSION     DADSZD010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.411"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..587)
                     /gene="fimE"
                     /locus_tag="J1Z28_004616"
     CDS             complement(<1..587)
                     /gene="fimE"
                     /locus_tag="J1Z28_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria regulatory protein FimE"
                     /protein_id="HBA7719351.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKR"
BASE COUNT          189 a          169 c          133 g          171 t
ORIGIN      
        1 tctcttttta atttttcgtt tatgagatta tttctttccc ataatccggc aaaacgagca
       61 gcattactgg cggtataacg cacagtatgg cgaatatttc gatgcccgag ataatcctga
      121 attaaacggg tatctgcacc acgttccgcc agttcataac cacaagcatg ccttaacata
      181 tgaggatgag tctgcgttac ggttccagct tcaataccgg catcgcgaat aatgcgatag
      241 gcctgctggc gagaaagccg actcccacgg cgagaaataa atatagcgtc ggtccggtca
      301 gcgcctttcc agttagcacg ttcctgggtc cagcgttcca cggcttcacg ctcatcaaaa
      361 cgtaacgggt gaacggtaga aaatccgttc ttcagtcggc gaatatttat tcgaccttca
      421 ttaaggtcaa gatcctgata atgcagatca agcagttcac taatacgcat cccatgccga
      481 tatgccaaca gaataagaca ataatctctg gctcccgttg ccccgtaaca caccgcctgc
      541 atcatagcct gaacttcttt accggtaaga taacgacgtt tactcacaat agtagtactc
      601 ctgactgaga tatatttgaa tgaataccta taggaaacct caatcggtca aaattagccc
      661 aa
//
LOCUS       DADSZD010000410          660 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.412, whole genome shotgun sequence.
ACCESSION   DADSZD010000410 DADSZD010000000
VERSION     DADSZD010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.412"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            341..605
                     /locus_tag="J1Z28_004617"
                     /pseudo
     CDS             341..605
                     /locus_tag="J1Z28_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          215 a          128 c          139 g          178 t
ORIGIN      
        1 agataacgca tataattcgc aattgttatg cattaaaaat gctaaaaaac aggggtactc
       61 agacgaatca gtcttgccac tttttctaca tatttcctac attcaggaaa aaggctggaa
      121 atggtatcaa tattatgact gtggatatat cgtaaaacaa gcatctgttt tctttccgac
      181 ataccaagca tgtatcattg atgctgaaca agaatgtatt ggtatactag ttcaactgtc
      241 tgaaaaaact tgagccgccc cttgaaacgc cagacagtgg atgtatctta aaataagaga
      301 taggcttgga tatatgtcta acactagttc taattttgat ataactggaa ctttgctggg
      361 gcaggagctt cgtaaacgta aaactcctca gaaaaaaaat tgccattact cagcagacaa
      421 cggagcctgg catgacggtg tcccatgtgg tgcatttgca tggtattcag cccagtctgt
      481 tgtttaagtg gaaaaaacaa taccaggaag gaagtctcac tgctgttgct gccggagagg
      541 atgttgttcc tgcctcagaa ctggctgccg ctataaaaaa taaaagagtt acagctcttg
      601 ctgggtaaaa aaacatggaa gttgagatcc tcaaaaaagc cgcggagtac accactctac
//
LOCUS       DADSZD010000411          660 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.413, whole genome shotgun sequence.
ACCESSION   DADSZD010000411 DADSZD010000000
VERSION     DADSZD010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.413"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..388)
                     /locus_tag="J1Z28_004618"
     CDS             complement(<1..388)
                     /locus_tag="J1Z28_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3990 domain-containing protein"
                     /protein_id="HBA7719352.1"
                     /translation="MTGPIEPSKGRVGLDFNPAGKGGFYVTTDKGQALDWATKRGHPS
                     ITQFDIPNSELAKLDIKIFDSASGEWSDFVTQGRQGTLSHNFDAVSGPMVANPGTVRR
                     GATPKPIGSQLAIFSDKAAALFDRFNS"
     gene            complement(425..640)
                     /locus_tag="J1Z28_004619"
                     /pseudo
     CDS             complement(425..640)
                     /locus_tag="J1Z28_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515766.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          177 a          177 c          126 g          180 t
ORIGIN      
        1 tcgagttaaa cctgtcaaat aacgcagctg ctttatctga aaaaattgct aactgacttc
       61 ctataggttt gggtgtagct cctcttctta ctgttccagg gtttgctacc ataggcccac
      121 ttacggcatc aaaattatgt gaaagtgtac cttgtcgtcc ctgagtcaca aaatcagacc
      181 actccccaga tgcagaatca aatatcttaa tatccaattt agctagctct gagtttggta
      241 tatcaaattg ggtaattgag ggatgaccac gctttgttgc ccaatctaga gcttggcctt
      301 tatctgtagt cacatagaag ccgccttttc ctgcaggatt aaaatctaaa ccaacacgcc
      361 cctttgaagg ttctatgggc cctgtaatat cccccgcatg gtagaaagta gtaacatcat
      421 ctttagcagt caaccctaat ggatcagccc aactcagtgt attcggagca taagcatata
      481 agtttaaccc accccgaatc gaaatcggat cctggcttac gaacctcccg cactccggcg
      541 cataatacct gaacagatta tagtgcagcc ccgtctcttc atcataatac tgccccggca
      601 gtcgcagcgg ctgctcaaaa tacgccccac tgttgctgat gtcgccccgg ttttcaccaa
//
LOCUS       DADSZD010000412          657 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.414, whole genome shotgun sequence.
ACCESSION   DADSZD010000412 DADSZD010000000
VERSION     DADSZD010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.414"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            124..330
                     /locus_tag="J1Z28_004620"
     CDS             124..330
                     /locus_tag="J1Z28_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719353.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
BASE COUNT          185 a          133 c          143 g          196 t
ORIGIN      
        1 tgcaaccgac agatgtatgt aaggtcgatg tactcaaact ttcatacttt tcctctttta
       61 tgcagaaaga tttgaagtaa tattttaacc gctagatgac gagcaaacgc atggagcgac
      121 aaaatgaata aagaacaatc tgctgatgaa ctctcgttgg atctgattcg tgtaaaaaat
      181 atgcttaata gcaccatttc tatgagttac ccggatgttg taattgcatg tatagaacat
      241 caggtgtctc tggaagcatt cagggcaatt gaggcagcgt tggtgaagca cgataagaat
      301 tcgaaggatt attccctggt ggttgactga gcaccataac tgctaatcat tcaaactatt
      361 tcacctgtga cagagtcaat atcgcattct gtcactgtca ggctaataca gagctgcaat
      421 tcaactactg caatgtcctc gtaattaggt gaatttacaa tatcgtcctg ttcggatgcc
      481 ggctgcattg ctgaagatga ggcatttatg gttcgcatat tttcccctca tgctcgtcag
      541 tcctgtgcgt aggaagaaac aggacactca cactaatttg tgtgggcatg ctgtgatgtc
      601 cttctgaatt attcctatgc cattatgtaa agcgctgtat cagatgctcg tcacggc
//
LOCUS       DADSZD010000413          646 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.415, whole genome shotgun sequence.
ACCESSION   DADSZD010000413 DADSZD010000000
VERSION     DADSZD010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.415"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..450)
                     /locus_tag="J1Z28_004621"
     CDS             complement(<1..450)
                     /locus_tag="J1Z28_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719354.1"
                     /translation="MKQNDTETRMATAKIIDHLSFGVTLIASPERVKQELCNATYSIL
                     GTKDSIPIDQLVWTKLSYIFGDYHPYNTSFDAAEELIIQKSFFDHMWDISLVEMMNHI
                     NHESWEQFDWQKTAEMLNLANKEHTNELRSYQHAYRIEFDGVLSLFNE"
BASE COUNT          188 a          120 c          111 g          227 t
ORIGIN      
        1 ttcgttgaaa aggcttaaca ctccgtcgaa ctcaatacga tatgcgtgtt gatagcttct
       61 aagctcattg gtatgctctt tattagccaa gttaagcatt tctgcggttt tctgccaatc
      121 aaattgttcc cagctttcat ggttgatatg gttcatcatt tcaacgagtg aaatatccca
      181 catatggtca aaaaaagatt tctgaatgat cagttcttca gctgcatcga aagaagtatt
      241 gtatggatgg taatcgccga aaatatagct tagtttggtc catactaatt gatcaattgg
      301 aatgctgtcc ttagttccca aaatcgaata cgtagcatta cataattctt gtttgactct
      361 ctcaggacta gctataagtg taactccaaa gctcagatga tcaataattt ttgctgtggc
      421 catccttgtt tcggtatcgt tctgtttcat tagttctaaa aaaacagtag cagatatagg
      481 gcaaaccacc tttccattat caaccaagta ctttattttt tgtaggagat taattaagtc
      541 ttgatcgtta ttattcccaa gctcaatatc tctaaaaaat atccagaacc ttaagtcgag
      601 ataaatcttg tattttttgg agatttcttt tccaagttgt agtcgt
//
LOCUS       DADSZD010000414          642 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.416, whole genome shotgun sequence.
ACCESSION   DADSZD010000414 DADSZD010000000
VERSION     DADSZD010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.416"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..408
                     /locus_tag="J1Z28_004622"
     CDS             <1..408
                     /locus_tag="J1Z28_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBA7719355.1"
                     /translation="SMEDAWQEIDMGTTPLRDLQNSPAGPEKKFRLRLRNCELTGAGK
                     QVYTATRVRVTFDGIPGETPDKFSLTGQAEGINLQIMDNYGYPARAGKSMPPLILRGN
                     EDGLDYSLRIVRNGYPLKAGDYYAALRFKLDYE"
BASE COUNT          179 a          133 c          158 g          172 t
ORIGIN      
        1 tccatggagg atgcctggca ggaaattgat atgggaacca cgccactcag ggacttacag
       61 aacagtcctg ccggtcccga gaagaaattc cggctacggc tacgcaactg tgagcttaca
      121 ggtgcaggaa agcaggtcta tacggcaacc cgggtgcgtg tgacttttga tggcattccc
      181 ggagaaacac cggacaaatt ctcgctgaca ggtcaggctg aaggtataaa tctgcagatt
      241 atggataatt acggatatcc ggcgagagca ggaaaaagca tgccaccttt aattctcagg
      301 gggaatgaag atgggcttga ttattctctt cgcattgtca gaaatggtta tccattaaag
      361 gctggcgatt actacgctgc attgcgtttt aaactggact acgaataaat acaaccggag
      421 tagccgggct cactgctatc agcgacagcg ttaataatac attcctgaat gttgaatagg
      481 gttagttgcg cccttcaaag tgtttttgta ttaaatcggc tgcatataat gaaaaaaaat
      541 aaatcgactt ttaccatcaa ttttattacg tattcattga tgctttctct ggcaggagtg
      601 cctgtctatg ccgtcgattt taatacggat gttctggatg cc
//
LOCUS       DADSZD010000415          641 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.417, whole genome shotgun sequence.
ACCESSION   DADSZD010000415 DADSZD010000000
VERSION     DADSZD010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.417"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>641
                     /locus_tag="J1Z28_004623"
     CDS             <1..>641
                     /locus_tag="J1Z28_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA7719356.1"
                     /translation="AGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDP
                     KRMIEACDENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGF
                     LAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLG
                     GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLG"
BASE COUNT          133 a          178 c          183 g          147 t
ORIGIN      
        1 gcaggcaaac caacggataa accaaacctg gtgtgcggtc cggtacaaat ctgctggcat
       61 aaattcgctc gctactggga tgtggagttg cgtgagatcc ctatgcgccc cggtcagttg
      121 tttatggacc cgaaacgcat gattgaagcc tgtgatgaaa acactatcgg cgtggtgccg
      181 actttcggcg tgacctacac cggtaactat gagttcccgc agccgctgca cgatgcgctg
      241 gataaattcc aggccgacac cggtatcgac atcgacatgc acatcgacgc tgccagcggg
      301 ggcttcctgg caccgttcgt cgccccggat atcgtctggg acttccgcct gccgcgtgtg
      361 aaatcgatca gtgcttcagg ccataaattc ggtctggctc cgctgggctg cggctgggtt
      421 atctggcgtg atgaagaagc gctgccgcag gaactggtgt tcaacgttga ctacctcggc
      481 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
      541 tattatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa cgcttcctac
      601 caggttgctg cttatctggc ggatgaaatc gccaaactgg g
//
LOCUS       DADSZD010000416          638 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.418, whole genome shotgun sequence.
ACCESSION   DADSZD010000416 DADSZD010000000
VERSION     DADSZD010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.418"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..396
                     /locus_tag="J1Z28_004624"
     CDS             <1..396
                     /locus_tag="J1Z28_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012442695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719357.1"
                     /translation="GLAGGLNLYAYAPNPLGWIDPLGLKCGQPEWTNHGYKHFPPKNK
                     SWKDIIKSTKSGPAKYSPDIDIKTLEYDVFNTGTPVTNGKPWKVKDMGKVIGASEGKE
                     SQWVRVELSGGTIHGHPISLDEFRRLTTP"
     gene            393..>638
                     /locus_tag="J1Z28_004625"
     CDS             393..>638
                     /locus_tag="J1Z28_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719358.1"
                     /translation="MNIDFLGKKGNVVFNDFSLKQSIPLNEQRDELKEDMLQVEYPGG
                     YLLDVGWRPSFNINGKFYICLIKDFDWDAPIYNGSAKD"
BASE COUNT          208 a          104 c          147 g          179 t
ORIGIN      
        1 gggctggcgg ggggattaaa cttatatgct tatgcgccta atccgctggg gtggatcgat
       61 ccgttaggtt taaagtgtgg gcaacctgaa tggacaaatc atggatacaa acattttcct
      121 ccaaaaaaca aatcatggaa ggatataatt aaatcaacaa aatcaggccc ggcaaaatat
      181 tctccagata ttgatattaa aacgctggaa tacgatgttt ttaatacagg cacacctgtt
      241 accaacggta aaccatggaa agtaaaagat atgggcaaag ttattggggc cagtgagggt
      301 aaagaatctc aatgggttcg agttgaactt agcggtggga caatacatgg acatcccata
      361 tcacttgatg aattccgaag gctaacaaca ccatgaacat tgatttctta ggaaaaaaag
      421 ggaatgtggt ttttaatgac ttttccctga aacaaagcat ccctcttaat gaacagcgag
      481 atgaacttaa ggaagatatg ctccaggttg aatatcctgg tgggtatctt ttggatgttg
      541 gatggcgacc atctttcaat ataaatggga agttttatat ttgtttaata aaggattttg
      601 attgggatgc tcccatttat aatggttctg cgaaagat
//
LOCUS       DADSZD010000417          635 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.419, whole genome shotgun sequence.
ACCESSION   DADSZD010000417 DADSZD010000000
VERSION     DADSZD010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.419"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>635
                     /locus_tag="J1Z28_004626"
     CDS             <1..>635
                     /locus_tag="J1Z28_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBA7719359.1"
                     /translation="YSLFNKNDDVIPEWLVSKFNTASVSTATLYYIETDHQQILTKER
                     ELVEFLSRKCGTRLESKLQRITCYVAFRMREEEHEKMLQRREKGWQPSEQNTVKSILD
                     VPDGKIVEFDASHSNLRREMAYESWHMQHCVGQFDDRKNLTGGYGEYYANQIEQHKLR
                     LFSLRDNNNIPHVTIALNVVGDSLEIDQIKGKQNRHPVKKYADDVLSLLQL"
BASE COUNT          208 a          108 c          134 g          185 t
ORIGIN      
        1 atattcactg tttaataaaa atgatgatgt aatcccggaa tggctggtat cgaagtttaa
       61 tactgcctca gtatcaactg cgacacttta ttatatcgaa accgatcatc aacaaatatt
      121 aaccaaagaa cgggagttgg tggaatttct ttccaggaaa tgtggtaccc gtctggaatc
      181 aaaactacag cgcataacct gctacgttgc gttcagaatg cgtgaagaag agcatgaaaa
      241 gatgcttcaa cgacgcgaga aagggtggca gccgagtgaa caaaatactg taaagtcaat
      301 acttgatgtg ccagacggaa aaattgttga gtttgatgcc agtcattcga accttcgtcg
      361 tgaaatggct tatgaatcat ggcatatgca acattgcgta ggccagtttg atgatcgaaa
      421 aaatcttacc ggtggttatg gtgagtatta tgcaaatcaa attgagcaac ataaattacg
      481 gttattcagt ttgcgtgata ataataatat tcctcatgtt actattgctc tcaatgttgt
      541 tggtgattca cttgaaattg atcaaataaa aggtaagcaa aatcgtcatc cagttaaaaa
      601 atatgcggac gatgttttgt ctcttttgca actat
//
LOCUS       DADSZD010000418          618 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.420, whole genome shotgun sequence.
ACCESSION   DADSZD010000418 DADSZD010000000
VERSION     DADSZD010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.420"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            220..549
                     /locus_tag="J1Z28_004627"
                     /pseudo
     CDS             220..549
                     /locus_tag="J1Z28_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          182 a          155 c          156 g          125 t
ORIGIN      
        1 agtacttata agtgaaaatc cgggataact gatcttacgg ttgaataaga tcgtcaataa
       61 acaccacacc atcagtagta cgcaaccagc gtgggagaca gaatgccaat cccgacgcct
      121 gattttgcag acgcagcata gcgatacaac gatcgcgaaa tggcgacaag tttctttccc
      181 ggaagacgtc tcgcaaaacc gggaaaaagt ggcagtgaca gcgaactggt gcgttatgac
      241 tatatcagcc acggcgatga gcagcggctg agtgatgtac agacccgctt taatggtgcg
      301 ttgcactata cctacaccga acagggctgg ctgagttcct ggcgcgacaa cggcccgaca
      361 catttccacc tgcgatatga cgatgaaggc cgggtgactg ccacaggcac acaggaagga
      421 ctgtataacg acaccttccg ctacttccca gaggagcgca gaaccgatta cactgatgcc
      481 accggagcca ccaccacgct gtggtttgat gagtcctggc tgctgaaaaa acagcgtgac
      541 ccgctggggc agggaaagaa catcagaagt acactttttg tactaaacaa tttacatttt
      601 atgtttaaaa attgagac
//
LOCUS       DADSZD010000419          609 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.421, whole genome shotgun sequence.
ACCESSION   DADSZD010000419 DADSZD010000000
VERSION     DADSZD010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.421"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            27..>609
                     /locus_tag="J1Z28_004628"
     CDS             27..>609
                     /locus_tag="J1Z28_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719360.1"
                     /translation="MLMLKKGMMLVGLIFFSLPANAGCTLEETARKVIMGFQQEDAQL
                     INSLIDKKTGLYVLFHRGASMDVENLKQINFRQPVPEYFPWPSAGEHVPRDDEFNKKA
                     IPKFNCERGWNKQGYFISNSDVHHKVSRSLVFTQMFGSPQNTISDAQIAAARRMEYGA
                     VRVVAAPEESNDGLVFYLSQLYSYDSDWYLTVVD"
BASE COUNT          169 a          116 c          158 g          166 t
ORIGIN      
        1 agccccatga tgagaagatg caaacaatgc tgatgttaaa aaaaggaatg atgctggtgg
       61 ggctgatatt cttttcttta cccgccaatg ccgggtgtac gctggaggaa acagccagaa
      121 aggtgattat gggctttcag caagaagatg cccaattaat caactcgtta attgataaaa
      181 aaacgggttt gtatgttctt ttccaccgtg gtgcttcgat ggatgttgaa aatcttaagc
      241 aaattaattt caggcaacct gttcccgagt atttcccatg gccctcggca ggggaacatg
      301 ttccgcgaga tgacgaattt aataagaaag ctatcccgaa atttaactgc gaacgtggct
      361 ggaataagca ggggtatttt attagcaata gcgatgttca ccataaagtc agccgtagtt
      421 tggtcttcac gcagatgttt ggttcaccgc aaaatactat ttctgatgcg cagattgccg
      481 cagcgcgtcg aatggaatac ggggccgtgc gtgtggtcgc tgcgccagaa gagagtaacg
      541 atggactggt attttattta agtcaacttt acagttatga cagtgactgg tatctgacgg
      601 tggtggatc
//
LOCUS       DADSZD010000420          597 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.422, whole genome shotgun sequence.
ACCESSION   DADSZD010000420 DADSZD010000000
VERSION     DADSZD010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.422"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..458
                     /gene="ompT"
                     /locus_tag="J1Z28_004629"
     CDS             <1..458
                     /gene="ompT"
                     /locus_tag="J1Z28_004629"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HBA7719361.1"
                     /translation="SYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYE
                     DFELGGTFKYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVY
                     VEGAWNRVTNKKGDTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
BASE COUNT          193 a          121 c          121 g          162 t
ORIGIN      
        1 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
       61 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      121 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      181 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      241 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
      301 aagtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtgat acttcacttt
      361 atgatcacaa tgataacact tctgactaca gcaaaaatgg tgcaggcata gaaaactata
      421 acttcatcac tactgctggt cttaagtaca ccttttaaca acgttaaata gattttctcc
      481 ccggtggtat tgtatagccc cggggaattt gtttaccgtt aaaagcaaac aatatcccct
      541 aatcttgtaa gcatttaacc tgcgtcctgg cgtgtaacct cttaccgcaa cacaaga
//
LOCUS       DADSZD010000421          594 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.423, whole genome shotgun sequence.
ACCESSION   DADSZD010000421 DADSZD010000000
VERSION     DADSZD010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.423"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            41..>594
                     /locus_tag="J1Z28_004630"
     CDS             41..>594
                     /locus_tag="J1Z28_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7719362.1"
                     /translation="MKTVFTTLSVIFGVIFSHSVLAQDVTIYYTNDIHAHVNPAKIIA
                     VDKNRLIGGMANIAGIVNEAKKKSKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMP
                     FDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
                     GVIGLHGAFAFYDTVAAKAREGVEA"
BASE COUNT          183 a          114 c          129 g          168 t
ORIGIN      
        1 ataattcaac aaatgtaaca acccctatcc aggagggatg atgaaaactg tatttacaac
       61 attatcagtc atattcggcg tgatattttc tcacagtgtt ctagctcagg acgttactat
      121 ttattatacg aatgacattc acgcacatgt caatccggca aaaatcatcg ctgttgataa
      181 gaacagactt ataggtggta tggctaatat tgcaggcatc gttaatgaag cgaagaaaaa
      241 aagcaaagat gtgtttttct ttgatgcagg cgactatttt accgggccgt atatcagcac
      301 actgaccaaa ggtgaagcta ttattgatat catgaacact atgccctttg atgcggtgtc
      361 tgttggtaat cacgagttcg atcacggtgt gcccaatatg gtgtctcagt tatcaaaagc
      421 aaaattcccc attctgctgg gaaatatcta ctacaccgat acgaataaac cagtttggga
      481 tcatccgtgg accattatcg aaaaagatgg tttaaagatt ggtgtgattg gattgcatgg
      541 cgcattcgct ttctatgata cagtcgcggc aaaagcacgt gaaggcgtag aagc
//
LOCUS       DADSZD010000422          590 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.424, whole genome shotgun sequence.
ACCESSION   DADSZD010000422 DADSZD010000000
VERSION     DADSZD010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.424"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..252)
                     /locus_tag="J1Z28_004631"
     CDS             complement(<1..252)
                     /locus_tag="J1Z28_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBA7719363.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGH"
BASE COUNT          148 a          117 c          164 g          161 t
ORIGIN      
        1 gtgaccgtgg ccactcagac ggaagcgccg tacttcccgg gtgccggcgt gaacatcact
       61 gaccggggtg acctccccct gaaacagtgc attacagagt gaggatttac cggccccgct
      121 tttacccata atgccaatca cgggttcgtg acgggtgagt ttgcgcagat gttccaggat
      181 gtgacgggaa agcgagtaag gcagggagga gagcggtttt tcaattgcct caatggcatc
      241 agacggattc atacatgtat tcccggtgaa aaaagacaaa accccgcgca ccggaaaggt
      301 ggcgggggat tcacggagat aatatctgtc tgaaattgtt ttgaatcgat gggattgttg
      361 cgtattaaca tgaaggagat caggacttat ccgttactgg gtgatctcct tccatgaaaa
      421 tacgccgtta ttgttaaaca gcaggctgat ttctccgtaa aggggaaaat atttatcggc
      481 tgcatgatta tcaggtgtgt ctgtgtttac tgctgtgagt atttttcagt gtattgtggg
      541 ggagatgtca gtgggagata ttaaatttac gtttataatt atcttacttc
//
LOCUS       DADSZD010000423          589 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.425, whole genome shotgun sequence.
ACCESSION   DADSZD010000423 DADSZD010000000
VERSION     DADSZD010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.425"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..499
                     /locus_tag="J1Z28_004632"
     CDS             <1..499
                     /locus_tag="J1Z28_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003843858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBA7719364.1"
                     /translation="NLIKVLQSCQGKVVFIGVGKSGIIARKLAATFASTGTPSFFVHG
                     TEAVHGDLGMVAKDDVVILISNSGETAEILATLPSLKKMGNYLISFTRSHHSSLAISC
                     DLSVEIPVKSEADNLGLAPSCSSTVVLVVGDAVALALSELKKFTRADFGLYHPGGALG
                     IKANS"
BASE COUNT          150 a          113 c          140 g          186 t
ORIGIN      
        1 aaatttaatt aaagtattac aatcttgtca gggaaaagtt gtttttattg gtgttggtaa
       61 gtccggtatt attgccagga aactcgccgc aacatttgcc agcaccggaa ctccctcgtt
      121 ttttgttcac ggtacggaag ctgtacacgg cgaccttgga atggtggcga aagacgatgt
      181 tgttattctt atttcaaaca gtggtgagac ggcggaaatc ctggcgacac tgcctagtct
      241 gaaaaaaatg ggtaattatt tgatctcttt tacccgcagt catcactcat cgcttgcgat
      301 aagttgtgat ttatctgttg aaatccccgt caaaagtgag gcggataatt taggtttagc
      361 accgtcatgc tcgtcgactg ttgttctggt tgttggtgat gcggtggcgc ttgcactctc
      421 tgaattgaaa aaatttactc gtgctgattt cggcttatat catccaggag gtgcactcgg
      481 cattaaagca aattcataat ttcaaaatat aaatacagta accggtcacg ccatcttttg
      541 gggtggcgtt ctttgaagtg agtgtccgct atgaaaaatg ttctggtca
//
LOCUS       DADSZD010000424          582 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.426, whole genome shotgun sequence.
ACCESSION   DADSZD010000424 DADSZD010000000
VERSION     DADSZD010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.426"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          197 a          103 c          114 g          168 t
ORIGIN      
        1 aaaatataaa acaattgttt actatctatg gcaagctcct ggattatgtg tgtgaactag
       61 gagtttctca ggaatttcct cacaatgtgg cactatcact gtcacctttt tctccttgga
      121 aacctccatg gatgcctcaa agtttcggtc agtttttaga taggtagcat tcctcattaa
      181 taatgaggac accgagggta gaatcttaac tccgtgttaa tagaataaga ataaaaaaca
      241 atcatcagaa ctgagtaggg aaagcgaatc atgatgtatg aagtttcaca tacaatgtga
      301 atcaaaaacg ctcgtagata attcgatagt gctgcctgat acgctgaaag aaattgtccg
      361 caggaagatc tgattatgct gattcgtcga ccacaggccc ttatatacat aagcagcgcc
      421 cgcgcctgaa atgcaggtca gaaacaatgt gaacattatc cgtgagaaat tgtgacaaaa
      481 ttcccgatga tcgacaagtg caatgaaata aaaaacatat gttatatatc aatttgatat
      541 ttatgaaaaa tcaatagatt tatcttgtga gggattgctt tt
//
LOCUS       DADSZD010000425          581 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.427, whole genome shotgun sequence.
ACCESSION   DADSZD010000425 DADSZD010000000
VERSION     DADSZD010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.427"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..237
                     /gene="cmi"
                     /locus_tag="J1Z28_004633"
     CDS             <1..237
                     /gene="cmi"
                     /locus_tag="J1Z28_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HBA7719365.1"
                     /translation="RTFVKNDYLQKMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDE
                     KTLLVPFKAEGPEGKRMYYGMYHCEEGYVEYAND"
     gene            complement(287..>581)
                     /gene="cma"
                     /locus_tag="J1Z28_004634"
     CDS             complement(287..>581)
                     /gene="cma"
                     /locus_tag="J1Z28_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin colicin M"
                     /protein_id="HBA7719366.1"
                     /translation="FPVSTKFTHATGDYNVITGAYLGNITLKTEGTLTISANDSWTYN
                     GVVRSYDDKYDFNASTHRGIIGESLTRLGAMFSGKEYQILLPGEIHIKESGKR"
BASE COUNT          191 a          105 c          129 g          156 t
ORIGIN      
        1 agaacgtttg taaaaaatga ttacctgcaa aaaatgaaac gctgggacaa cgatgttcaa
       61 cttcttggta cagaaatccc gaaaattaca tgggaaaaga ttgagagaag tttaacagat
      121 gttgaagatg aaaaaacact tcttgtccca tttaaagctg aaggcccgga gggtaagaga
      181 atgtattatg gcatgtacca ttgtgaggag ggatatgttg aatatgcgaa tgactaatac
      241 actgttataa aggctgcata aaaaggccgg aatcccggcc cttatattat cgcttaccac
      301 tttctttaat gtgaatttca ccaggaagca gtatctggta ctctttacca gaaaacatcg
      361 ccccgagcct tgtgagcgac tctccgatga tgccacggtg agtgctggcg ttaaaatcgt
      421 atttatcatc atatgaacga acaacgccat tgtaagtcca ggagtcattg gcagagatag
      481 ttaaagtacc ttctgttttc agtgtgatat taccaagata tgcaccggta ataacattat
      541 aatcaccagt ggcatgtgtg aactttgtag aaacagggaa t
//
LOCUS       DADSZD010000426          577 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.428, whole genome shotgun sequence.
ACCESSION   DADSZD010000426 DADSZD010000000
VERSION     DADSZD010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.428"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(79..414)
                     /locus_tag="J1Z28_004635"
     CDS             complement(79..414)
                     /locus_tag="J1Z28_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004856597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBA7719367.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT          144 a          135 c          134 g          164 t
ORIGIN      
        1 caagcgtcgc atcaggcatc aggcatcgtt gccggatgcg gcgtgagcgc cttatccggc
       61 ttaccgatca caatacaatc agttcttttt cacaaactca gatttcagtt tcatcggacc
      121 aaaaccgtcg attttgcaat cgatgttatg gtcgccttca accaggcgga tgtttttcac
      181 tttggtgcca attttcagca tcgaagagct acctttcacc ttcagatctt taatgatggt
      241 cacgctgtcg ccgtcagcca gcagattgcc gttagcatct ttaacgatta gctcgtcgct
      301 ttcctgtgca ggttctgcgt cgttccattc gtaggcacat tccgggcaga tgtacatgcc
      361 gttatcttcg taggtgtatt cggagttgca ttttgggcag tgtggtaatg acatgtggat
      421 ttcctcaaaa gtcagcaggg caaaagcgcc aaaaaacggc agattgccga aaaaggccgc
      481 aattatacac aaaatccctg agtttgtcgg gggtactgct gattttagac gacgatcgtt
      541 ttcatttgaa aggaaacgca aaaagaaaat aatcttt
//
LOCUS       DADSZD010000427          571 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.429, whole genome shotgun sequence.
ACCESSION   DADSZD010000427 DADSZD010000000
VERSION     DADSZD010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.429"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>571)
                     /locus_tag="J1Z28_004636"
     CDS             complement(<1..>571)
                     /locus_tag="J1Z28_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719368.1"
                     /translation="GGDDISLLKQQFDAGESSGNKYYSVIYKQNMRVRSKTLRQEMGT
                     GKFTRWEIQRGRTQDEINSIMLAMMMNTALKFEAYQSLKEFSWHTDSGFSGEDLVSDL
                     FGFYRFMIPGKYSSLVRPVNYNDAVSRWDFYGPIGSFKNDGFRPILFPDPKDPCVKHE
                     PYKADLPHFMTWIQPWSDFTSGKIRIITND"
BASE COUNT          172 a          138 c           87 g          174 t
ORIGIN      
        1 catcattcgt tataattctt atcttacctg atgtaaaatc actccacggt tggatccatg
       61 tcataaaatg aggtaaatca gccttataag gttcatgttt gacacaggga tccttcggat
      121 caggaaacag aataggtcga aacccatcat ttttaaagct accaataggt ccgtaaaaat
      181 cccagcgact gaccgcatca ttataattga cgggacgaac caggctactg tatttaccag
      241 gaatcataaa acgataaaaa ccaaaaagat cagaaaccaa atcctcacca ctaaatccac
      301 tatctgtatg ccatgaaaat tctttcagac tttgatatgc ttcaaacttt aatgctgtat
      361 tcatcatcat cgccagcatt atgctattaa tttcgtcctg ggtgcgtcct ctttggattt
      421 cccagcgagt gaatttccct gttcccattt cctgcctcag tgttttactt ctgacccgca
      481 tattctgttt atatatcact gagtaatatt tattaccgga actttcaccc gcatcaaatt
      541 gctgtttcaa caaggaaata tcatcccccc c
//
LOCUS       DADSZD010000428          564 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.430, whole genome shotgun sequence.
ACCESSION   DADSZD010000428 DADSZD010000000
VERSION     DADSZD010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.430"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>564
                     /locus_tag="J1Z28_004637"
     CDS             <1..>564
                     /locus_tag="J1Z28_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBA7719369.1"
                     /translation="GTISEKELEFYKRRSQNVGLVITGCTYVTPSGIGFTHEFAAYDD
                     RFINSLEKLAAAAKSGGAPAILQIFHAGNKAIPELVPNNDVISASASSVKSGDFMKRV
                     VQSREMTENEIQETIRAFGDVTKRAIKAGFDGVELHGAHGFLLQNFFSPLFNQRNDRW
                     GGDLEGRMRFPLAVLQEVKNVVYEYAT"
BASE COUNT          169 a          115 c          137 g          143 t
ORIGIN      
        1 atggaaccat ctctgagaaa gaactcgaat tttataaacg tcgctcacaa aatgttggtt
       61 tagtaataac aggatgcacc tatgtcacgc caagcggcat tgggtttact catgaatttg
      121 ccgcgtatga cgaccgtttc attaacagcc ttgaaaagtt agcagctgca gccaaaagtg
      181 gcggtgctcc ggctattttg cagattttcc acgccggtaa taaagccatc ccggagcttg
      241 tgccaaataa cgatgttatt agtgccagcg catcaagcgt aaaatctggt gactttatga
      301 agagagtggt acaaagcagg gaaatgactg aaaacgaaat tcaggaaacc attagagcat
      361 tcggagatgt cacaaaaagg gcgattaaag caggttttga tggcgttgaa cttcatggtg
      421 cgcacggatt ccttctgcag aatttcttct cccctctatt taaccagcgt aatgaccgat
      481 ggggaggaga tcttgaagga cgtatgcgct tcccgctggc cgtattacag gaagtaaaaa
      541 atgtagtcta tgaatatgcg acaa
//
LOCUS       DADSZD010000429          564 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.431, whole genome shotgun sequence.
ACCESSION   DADSZD010000429 DADSZD010000000
VERSION     DADSZD010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.431"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..511
                     /locus_tag="J1Z28_004638"
     CDS             <1..511
                     /locus_tag="J1Z28_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBA7719370.1"
                     /translation="AAVSAAEGDESVTTTVNGGVIHFKGEVVNAACAIDSESMNQTVE
                     LGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAFLGTTVTSNDDTLALQSSA
                     AGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILPFQARYIALGQSVAGTANA
                     DATFKVQYL"
BASE COUNT          140 a          119 c          147 g          158 t
ORIGIN      
        1 tgctgcggta agcgcagccg agggtgatga atcagtaacg accactgtta atggcggtgt
       61 aattcatttt aaaggtgaag tggtaaatgc cgcttgtgcg attgattcag aatcaatgaa
      121 ccaaacggtt gagctgggtc aggtccgttc ttctcgtctg gctaaagcgg gtgacctcag
      181 ctccgccgtt ggcttcaata tcaagctgaa tgattgtgat accaatgttt ccagtaatgc
      241 agctgttgca ttcctgggta ctactgtcac cagtaatgac gacacattag cgctgcagag
      301 ttcagcggca ggctctgccc aaaatgtcgg tatccaaatt ttggaccgta cgggggaggt
      361 attaatactt gatggtgcca cttttagcgc taaaactgac ttgattgatg gcacgaatat
      421 actgccgttc caggctcgtt atattgctct cgggcagtca gtagctggta ctgcaaacgc
      481 tgatgcgacc ttcaaagttc aatatctata attcactctt aaggtgacta tgcgtcatct
      541 gaaattgaaa cagggtgtgt gtgg
//
LOCUS       DADSZD010000430          563 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.432, whole genome shotgun sequence.
ACCESSION   DADSZD010000430 DADSZD010000000
VERSION     DADSZD010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.432"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            136..498
                     /locus_tag="J1Z28_004639"
     CDS             136..498
                     /locus_tag="J1Z28_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBA7719371.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          173 a          134 c          134 g          122 t
ORIGIN      
        1 taatcaatgt attgggtgat tttttaacgc attaatggtt acaatctgga actattacaa
       61 tgccatcagt tgtgaacaaa gcacctggtc gcgcatacta gaggctataa atttatctct
      121 atcagaagtc atcacatgaa agaagtcgaa aaaaacgaaa tcaaacgtct cagcgatcgt
      181 ctggacgcca tccgtcacca gcaggccgat ctatcgctgg ttgaagccgc agacaaatat
      241 gccgaactgg aaaaagagaa agccacgctg gaagcagaaa ttgctcgcct gcgcgaagtt
      301 cacagccaga aactcagcaa agaagcgcag aaactgatga agatgccgtt ccagcgtgca
      361 atcaccaaaa aagagcaggc tgatatgggc aagctgaaaa aaagtgttcg cggactggtg
      421 gttgtgcacc caatgaccgc gctgggccgc gaaatgggcc tgcaagagat gacagggttc
      481 tcaaagactg cgttttaagt tcctttctcg tcgctgtaat cacagcctct caccatttac
      541 tcgctcctgc tggcacatac agg
//
LOCUS       DADSZD010000431          557 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.433, whole genome shotgun sequence.
ACCESSION   DADSZD010000431 DADSZD010000000
VERSION     DADSZD010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.433"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            1..470
                     /locus_tag="J1Z28_004640"
                     /pseudo
     CDS             1..470
                     /locus_tag="J1Z28_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          171 a          115 c           97 g          174 t
ORIGIN      
        1 acgcctatta aagctaacca tttgcgaagc atactggatt atattgaaca ggaacagtta
       61 actgccgaaa aacgtaacca ttgcatgaaa ctgtccaaaa aaattcatag agaaaaaacc
      121 attcagccaa cagtaaatct caatgatagt gcattttttt gcaatctcct tctgacacta
      181 ttttttgtcg ccatccgtca ttacaatacg cccttgattc attgagaaat ggaaaaggca
      241 aagtcaacct gattaaacat tactcctctg ttgaatccat acagcagcat gtctccttag
      301 tccgggacgc ggagttcaga gcattacttc gccatcctcc tgcaaggagt cgcgttatcg
      361 cgagtcagga ttttggcttt gctttagata ttttcttctg tcgaatgatg gcaaacaatg
      421 tcagtcatat gtccgcgatt ttatatatag acaatcatac tttgtcagta agtaaagatg
      481 tcgtgttatc ttatatatca acgttgggta cttttgaaaa aaatattctt ttagccaacg
      541 tcagttattt acactgt
//
LOCUS       DADSZD010000432          550 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.434, whole genome shotgun sequence.
ACCESSION   DADSZD010000432 DADSZD010000000
VERSION     DADSZD010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.434"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..419)
                     /locus_tag="J1Z28_004641"
     CDS             complement(<1..419)
                     /locus_tag="J1Z28_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBA7719372.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWR"
BASE COUNT          157 a          119 c          117 g          157 t
ORIGIN      
        1 cgccatttca tcgccatccc gccgagcata caggcctcgg aagaaccaat ggtgttggtg
       61 ccaacggcct gaccattttt cggtgcaggc gcatgccaca gatcggcaac catgtttacg
      121 caacgcagat cgattgctgc agattgcgga tattcttctt tgtcgatcca gtttttgtta
      181 atggataaat ccatcaactt atggacatta tcgtcgtccc aggtctggca gaaagtggcc
      241 aggttctgac gagcgttgcc atcaagatat aattcatcat tgataatctg gaatgcgaca
      301 tcatcacgca tttcgtgcag cggaaaacgt tttgattctg cgatagtgga aatagacttc
      361 gcaccaaaac gtgaatcgag tagttccgac cttaaatccg ttacttgctt cttatccatt
      421 ttaaactcct taaaatgatt ggatcgcatt aaaaaagtag gatttatcga taaagtaagc
      481 aagtgcaaag gactcggttt taaataacaa aatcctaatg ttatttatcg cgagatattg
      541 cgtgaataat
//
LOCUS       DADSZD010000433          548 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.435, whole genome shotgun sequence.
ACCESSION   DADSZD010000433 DADSZD010000000
VERSION     DADSZD010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.435"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..324
                     /locus_tag="J1Z28_004642"
     CDS             <1..324
                     /locus_tag="J1Z28_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBA7719373.1"
                     /translation="AVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMT
                     RLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVACTVLRAVRDTVVSVARAVW
                     NWIFF"
BASE COUNT          115 a          152 c          143 g          138 t
ORIGIN      
        1 gcggtattcc gtctgttccg gcccgtacat ccggttgtgg ccgtatcggc ccgcaccggc
       61 tgggaactgg atacgctggt cagtgcgctc atgacagcgc ttcccgacca tgccgccagt
      121 cccctgatga cccgcctgca ggacgagctg cgcacggaat ctgtccggtc tcaggcccgt
      181 gaacagttta ccggtgcggt ggaccggata tttgacacag cggagagcgt ctgtattgcc
      241 tctgttgcat gcacggttct gcgtgccgtc cgtgacacgg tggtctctgt tgcccgcgct
      301 gtatggaact ggattttctt ctgaacactc tgccagggga aatgcctgat tacattgcgt
      361 taaccaactg aaaacatgac acaaacacct catacccctc ctgtttctct tgcgtggccg
      421 ccttgttatt aatagaataa aacgattgat aaaacaggta tcgatcgtta ctatcgatcg
      481 tttatagtgg ttaatggcct gcgaaaaggc agctgaatct cttcatcatg cagaacggaa
      541 ttgcacac
//
LOCUS       DADSZD010000434          547 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.436, whole genome shotgun sequence.
ACCESSION   DADSZD010000434 DADSZD010000000
VERSION     DADSZD010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.436"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            25..360
                     /locus_tag="J1Z28_004643"
     CDS             25..360
                     /locus_tag="J1Z28_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBA7719374.1"
                     /translation="MAELGRRITALRKDAGMTQTQVAQALNVSQQAVQAWEAGRRRIQ
                     ISILPAVARLLAVSLEDLLGEEPEKIARKRGPAPKWQQLIEEIDSLPKAKQKMISEML
                     SALIVQARN"
     gene            445..>547
                     /locus_tag="J1Z28_004644"
     CDS             445..>547
                     /locus_tag="J1Z28_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin SymE, type I toxin-antitoxin system family
                     protein"
                     /protein_id="HBA7719375.1"
                     /translation="MAEHDITPEVTLSKTQRRLKVGYVGISHTNRKTK"
BASE COUNT          159 a          139 c          150 g           99 t
ORIGIN      
        1 ataagcaacg aagaacaggt atttattgcc gaacttggca gacggataac cgcactgagg
       61 aaggacgccg ggatgacgca gacgcaggtc gcacaagcgc tgaacgtctc ccagcaggcc
      121 gtacaggcat gggaagccgg acggcggcgg atacagatct ccatactccc agccgtggca
      181 agactgttag cggtttctct ggaggatttg ctcggcgaag aaccggagaa gatcgcccgt
      241 aagcgtggcc cagcaccaaa atggcagcag cttattgaag agatcgacag cctgccgaag
      301 gcaaagcaga agatgatttc ggagatgtta agtgccctga ttgtgcaggc gcgtaactga
      361 ctgataaata atgcggtgat taagggcggc aacccttaat caccgctcac cacaagcaac
      421 taactgaggt agtggtagtt aatcatggct gaacacgata ttacgccaga agtcaccctt
      481 tccaaaacac agcgtcgcct taaagtcggc tatgtaggca tttcccacac caaccgtaag
      541 accaaag
//
LOCUS       DADSZD010000435          541 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.437, whole genome shotgun sequence.
ACCESSION   DADSZD010000435 DADSZD010000000
VERSION     DADSZD010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.437"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(50..202)
                     /locus_tag="J1Z28_004645"
     CDS             complement(50..202)
                     /locus_tag="J1Z28_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719376.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMS"
     gene            220..411
                     /locus_tag="J1Z28_004646"
     CDS             220..411
                     /locus_tag="J1Z28_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBA7719377.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          154 a          129 c           87 g          171 t
ORIGIN      
        1 atatatcaaa caaaataacc ctgattaatg aattattacg tttatcatgt taactcatca
       61 ttattacatc atcattgtaa ataattaaat taacttccat aacattaaaa tatgtatcca
      121 ctgacgcttt tttacataac gaagaattga ccattttgtc ctgttgtgcc ttaatgtaag
      181 taccgtccac ggcgtgggac atacttcaag gaaccttttg tgagtcaggc aaccagtatg
      241 cgaaaacgac accgatttaa cagtcgcatg acccgtatcg tactgctcat cagctttatc
      301 ttcttctttg gccgttttat ctactcgtcc gtcggtgcct ggcagcacca tcagagcaaa
      361 aaagaagctc agcaatccac actctccgtc gaatcaccgg tacaacgtta gcggttacct
      421 tctccactct cacagaacat aacggcacct cgctgtcgga tgcttttgct ctttgagatt
      481 atcaaagcgg cagatattct ttcatcttaa attttacgtc tttgttctga ctgaagttca
      541 t
//
LOCUS       DADSZD010000436          538 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.438, whole genome shotgun sequence.
ACCESSION   DADSZD010000436 DADSZD010000000
VERSION     DADSZD010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.438"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(1..518)
                     /locus_tag="J1Z28_004647"
                     /pseudo
     CDS             complement(1..518)
                     /locus_tag="J1Z28_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT          176 a          121 c           99 g          142 t
ORIGIN      
        1 tcaggttctc gctatccatc gccagcgatt ttgacagtaa agaaaaacgt tccagaccaa
       61 gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg aaggttttac
      121 catcaaaaga gataaaccct gtatttttga catgtcgata cttaacaacc tgcataatat
      181 ctgcacaata atttttctcg aactccttta cctgaataca ttcatcactg gaaatcagat
      241 aaggaccgac atcgtacaaa taagagggtg aaaccgagtc ggcgtaagcc aaccaacagc
      301 tgttcctccg taggatttag cgtgttgatg agatcaattg ccacactacg ccgatattca
      361 gcaatatttg ccaccgcaaa agcagtatca ataacggact gtttcaggcc atacacccgt
      421 tcctttacca tttgagtgta gatcccgcag gcaactgctg aaatcatgat aaaaatcagc
      481 agactgactg taagtacgaa tttattgatt ctggacatac gtaaactact cttttact
//
LOCUS       DADSZD010000437          536 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.439, whole genome shotgun sequence.
ACCESSION   DADSZD010000437 DADSZD010000000
VERSION     DADSZD010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.439"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>536)
                     /locus_tag="J1Z28_004648"
     CDS             complement(<1..>536)
                     /locus_tag="J1Z28_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719378.1"
                     /translation="KYTLEERSIACRNAWYLKSYDINEAGQVHVYIGDLARLPYEEQL
                     YWQAFNEWPKGTISKRAFQTDIVGEWYLSYEPLLALKHKISLMDKNPPIWWKPRGTVL
                     SDAVQYPATDSIKEWGDEILALDQYLVEGFLPKPLSEIAKYEGRTIEPKWGSLRILQE
                     ALIAKSATVDEAKMLVQP"
BASE COUNT          150 a          124 c           96 g          166 t
ORIGIN      
        1 ataggctgaa ctaacatttt cgcttcatct actgttgcgc ttttagcaat aagagcttct
       61 tgtagtatgc gtaaggatcc ccatttgggt tcaatggttc ggccctcata tttagcaatt
      121 tcactcagtg gctttggtaa aaagccctca actaaatact gatcaagcgc aaggatttca
      181 tcaccccact ctttgattga atccgttgca ggatactgaa cagcgtcact caatactgta
      241 ccccgtggct tccaccagat gggaggattc ttgtccatta gcgaaatttt gtgtttgagt
      301 gccaacaatg gttcatagga cagataccat tccccaacaa tatcagtctg aaatgcacgc
      361 ttagaaattg ttccttttgg ccattcattg aaggcttgcc aataaagctg ctcttcatat
      421 ggtaaccgag ccaaatcacc aatgtataca tgaacttgcc cggcctcatt aatatcatag
      481 gattttaaat accaagcatt tcgacaagca atacttcttt cttctaaagt atattt
//
LOCUS       DADSZD010000438          534 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.440, whole genome shotgun sequence.
ACCESSION   DADSZD010000438 DADSZD010000000
VERSION     DADSZD010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.440"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..471
                     /locus_tag="J1Z28_004649"
     CDS             <1..471
                     /locus_tag="J1Z28_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719379.1"
                     /translation="ISQDPISIRGGLNLYQYAPNPISWIDPLGLTVTFTDSRGLTLDV
                     SGYTDLSHLSDSELNRIIFMNSNDAPHPFGLSPRDRQGNVIVLHHERQQSQGPITAMP
                     DKHHSSSTNNTGQHPNGNKKGSGINNRQEFNLWKKEFWKSQAEKVLSNRGGGCV"
BASE COUNT          180 a           95 c          116 g          143 t
ORIGIN      
        1 atcagccagg atccgatttc gatccgtggc ggtctgaatc tttaccagta tgcgcccaat
       61 ccaataagtt ggatcgatcc tctgggttta acagttactt ttactgatag cagaggatta
      121 actttagatg tatcaggtta cacggattta agtcacttaa gtgattccga gcttaataga
      181 attattttta tgaacagtaa tgatgctcct catccatttg gtttatctcc gcgcgatagg
      241 caagggaatg taatagtatt gcaccatgag aggcagcaat cacaagggcc aattactgct
      301 atgccagaca aacatcatag ttcaagtaca aataacaccg gtcagcaccc aaatggaaat
      361 aaaaaaggtt caggtataaa taatcgtcaa gaatttaacc tgtggaaaaa agaattctgg
      421 aaatcacaag ctgaaaaagt tttaagcaat agagggggcg gttgcgtatg aatgaagaat
      481 tgttagcaag agtcagagat gagataacat ctgaccaaga tatggtccta tatg
//
LOCUS       DADSZD010000439          526 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.441, whole genome shotgun sequence.
ACCESSION   DADSZD010000439 DADSZD010000000
VERSION     DADSZD010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.441"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..429
                     /locus_tag="J1Z28_004650"
     CDS             <1..429
                     /locus_tag="J1Z28_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBA7719380.1"
                     /translation="RLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCL
                     ALGGIDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQ
                     KQVVFERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          165 a          106 c          121 g          134 t
ORIGIN      
        1 cggttgcttg ctcgcctgaa atgtggtgat caattgattg tgacaaaact ggatcgcctt
       61 ggttgtaatg caatggatat caggaaaaca gtggaacaac tgaccgaaac aggtatcaga
      121 gtgcattgct tagcattggg tggcattgac ctgaccagtc caacaggaaa aatgatgatg
      181 caagtaattt cagcagtcgc tgaatttgaa cgagaccttt tacttgaacg cactcattcc
      241 gggatagtaa gagcccgcgg cgcagggaaa cgttttggtc gaccacctgt gttaaatgaa
      301 gaacagaaac aggtggtatt cgaacgaatt aagtcaggtg taagtataag tgccattgcc
      361 cgggaattca aaacctcgcg gcaaaccatt ttaagagcca aagcaaaact tcagacacct
      421 gacatataaa aaataatctc ggtgtgagat gctttacgtc ttccaagccc ccttccttgc
      481 cgtaaatgga aagatacatc taattataga atttatatgt tttacc
//
LOCUS       DADSZD010000440          525 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.442, whole genome shotgun sequence.
ACCESSION   DADSZD010000440 DADSZD010000000
VERSION     DADSZD010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.442"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>525)
                     /locus_tag="J1Z28_004651"
     CDS             complement(<1..>525)
                     /locus_tag="J1Z28_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HBA7719381.1"
                     /translation="GLGNNDTGVIAKTDRAISLLRETLAEIIVDFKDAKLVIKRLQFD
                     VFGFSRGAAAARHFTNRVFERDPVLIKTITTAFQSVEYLGKPSGEVQFLGIFDTVAAI
                     GGLMDGFDPHDGNNFPVKVKLPPGVAKHVFQLTAMHECRYNFCLNSIKESWPELSFPG
                     SHADIGGGYDPLED"
BASE COUNT          150 a          132 c          100 g          143 t
ORIGIN      
        1 tcatcttcca gtgggtcata accgccacca atatcagcat gtgatccagg gaaacttaat
       61 tctggccagc tttcctttat gctattgaga caaaagttat agcggcattc gtgcattgct
      121 gtaagctgga atacatgttt ggccacacct ggcggtaact tcacttttac cggaaagtta
      181 ttaccatcat gagggtcaaa accatccatc agccctccga tggctgcaac tgtgtcaaaa
      241 atacctaaaa attgcacttc cccggaaggt ttacctaaat attcaactga ctgaaatgcc
      301 gtcgtaatgg ttttaatcag tacgggatca cgctcaaata cgcgattagt aaagtgccgt
      361 gcggctgctg ccccacgact aaagccaaat acgtcaaact gtagacgttt aattactagt
      421 ttagcatcct taaaatcaac aataatctct gccagtgtct cacgaagcag tgatatcgca
      481 cggtcagttt tggcaattac cccagtatca ttatttccca gcccc
//
LOCUS       DADSZD010000441          517 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.443, whole genome shotgun sequence.
ACCESSION   DADSZD010000441 DADSZD010000000
VERSION     DADSZD010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.443"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(80..>517)
                     /locus_tag="J1Z28_004652"
     CDS             complement(80..>517)
                     /locus_tag="J1Z28_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBA7719382.1"
                     /translation="SALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLF
                     YRPEGLSGISSDAPLALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDM
                     TMIAPFSNDVIKVNGMSGRTSSAKVHFFAINDFGGSIEGNANL"
BASE COUNT          127 a          114 c          120 g          156 t
ORIGIN      
        1 aaacgtggcg atgtgggtaa ggtgattagt agatttaata gtcacgttct gcttcctgct
       61 gtgttatatc cttgccagat taaaggttgg cgttgccttc aatagatcca ccaaaatcat
      121 taatggcgaa aaaatgcact tttgcagagc ttgttctgcc actcatccca tttaccttga
      181 tgacgtcgtt actgaacggc gcaatcattg tcatatccag gggatagcgt ttgccgttgg
      241 cttctaaatc accactgcta aaagagacat agtagggcgt agggttatca acgcgtaatg
      301 ctgctttgcc ttcagatgtt gcccaggacc acttcagagc caatggtgca tcagatgaaa
      361 ttccactcaa tccttccggg cgatagaaca gttttatacg tgtgcgaaat gccagttgta
      421 gcaggctttg atttgctgct tttgctgcat cgggttttgg tggaacttcc agtacgttaa
      481 accagaacac actttctctg tcttttggca gagcact
//
LOCUS       DADSZD010000442          515 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.444, whole genome shotgun sequence.
ACCESSION   DADSZD010000442 DADSZD010000000
VERSION     DADSZD010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.444"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..449)
                     /locus_tag="J1Z28_004653"
     CDS             complement(<1..449)
                     /locus_tag="J1Z28_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBA7719383.1"
                     /translation="MLAVSIAFLHLRFNTLSGTDKMRLEMYKSTLYSTIEQFYVLPYM
                     LSTDHIIRQAVITPDDMTSSELNQRIAHFNTQLKTAAIFILDTQGKAIASSNWQDPGS
                     YVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTPGFFLSTSIKDKGKIV"
BASE COUNT          153 a           88 c          120 g          154 t
ORIGIN      
        1 acaatttttc ctttatcttt tatacttgta gagaggaaga atcccggtgt atttgtagtg
       61 ctaccaatac cgtaaaagcg tccatttaag ccagacatgg cgtgtttata ataggggcga
      121 tagctataat tttgccctac atagctgccg gggtcctgcc agttgctaga agcgatggcc
      181 ttaccttggg tatccagaat aaatattgct gcggttttga gttgagtatt gaaatgtgca
      241 attcgttgat tgagttcgct ggacgtcata tcgtcaggcg taattaccgc ctgacggatg
      301 atatggtctg ttgagagcat atagggtaaa acataaaatt gctcgatggt ggaatataat
      361 gtggacttat acatttcaag ccgcatttta tcggtaccgg acaaggtatt aaaacgcaag
      421 tgcaaaaaag cgattgaaac cgcgagcatg ataagaaaaa gtgaaatgct tgtaatgtga
      481 aatcgccatt tgttcttaaa tatataattc cacga
//
LOCUS       DADSZD010000443          514 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.445, whole genome shotgun sequence.
ACCESSION   DADSZD010000443 DADSZD010000000
VERSION     DADSZD010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.445"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>514)
                     /gene="cadC"
                     /locus_tag="J1Z28_004654"
     CDS             complement(<1..>514)
                     /gene="cadC"
                     /locus_tag="J1Z28_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator CadC"
                     /protein_id="HBA7719384.1"
                     /translation="LIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKR
                     GYKLMVPVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTTTPPEQSPVKSK
                     RFTTFWVWFFFLLSLGICVALVAFSSLETRLPMSKSRILLNPRDIDINMVNKSCNSWS
                     SPYQLSYAIGV"
BASE COUNT          147 a          106 c          116 g          145 t
ORIGIN      
        1 cacgcctatc gcgtaagaga gctgatacgg agaactccag ctgttacaac tcttattaac
       61 catattaatg tcaatatcgc gtggattgag caaaatgcgc gatttactca taggaagacg
      121 tgtttcaaga cttgaaaacg ctaccagtgc tacacagata cctaacgaca acaggaaaaa
      181 aaaccatacc caaaaggtag tgaatcgttt gcttttaact ggggattgtt caggtggcgt
      241 tgtggtgttt tgaatgttaa gactgtggga gggagaatct gtggcaggaa ccgcctctgg
      301 tataggggga ggcgaagata gcattatttc ctctccctct tcttcgctgt accagataac
      361 cggcaccatt aatttatagc cgcgctttgg tacagtagcg atatagacag gactatcttc
      421 atcattatct tttaatgact tacgtagttc tgagatactc tgcgtcacaa cgtgattggt
      481 gacaatactt ctcttccaga cattatcgat aagt
//
LOCUS       DADSZD010000444          510 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.446, whole genome shotgun sequence.
ACCESSION   DADSZD010000444 DADSZD010000000
VERSION     DADSZD010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.446"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT           92 a          160 c          174 g           84 t
ORIGIN      
        1 gcggcgtcat cgtgtggccg tcagtcaaaa gaggttgtcg gcctggcggc ctccgcttaa
       61 aaggacttta tgcccgcccg gcgtcgtctg gctgcggcgg ctcgtgccac gcaaaaggcc
      121 gcgcgtcact cgttcccgcc tcgccagctt ccgctgcccg ccccggtccg gcgtggtcat
      181 gcaaaggcaa accccggtca ggcagcggtg ccacagagcg ccgttccgtc gcttgcggac
      241 tctctgccgc ccgcacccgc agaccccgcg ccgtggcccc gccatgccgc cggaggcggc
      301 ctggcggccc cccgtcacgg gttgccggaa acggacttag tggaaagcgg aatgacagat
      361 ccgtggcggt ggtgatatgg tgagcgggcg caaaaacgcg tgtcgggaaa gctgggttaa
      421 cgtgctgtta aataagtaaa aggtgatagc cgggaagcca gaaacggcgc gggcggtagc
      481 gtgttaaaaa gtatgacctg cactggatcg
//
LOCUS       DADSZD010000445          509 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.447, whole genome shotgun sequence.
ACCESSION   DADSZD010000445 DADSZD010000000
VERSION     DADSZD010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.447"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          180 a          100 c           95 g          134 t
ORIGIN      
        1 ggtaaaaaat atttttaata ctgaaggtat aaagccataa ccagatattg caattaacgt
       61 agcgttacac atatcaacgt actacatgag taacagaggt ataggattga acaacactcc
      121 ggaagacttt ttaatgacat gaggcatact cttttacgta ttactaccaa aactcatcag
      181 gtgagcagca ggaacagatc attaacagta attccttaat caccgttatc cacagtatca
      241 acactggcga agtcatactc ttatttagcc agaaacactt acacaaacgc acaaaagcaa
      301 tggcggaccc agaatagtct ccacacaaca ggcattactt aacaaagtac gttacagtca
      361 gtgtgtgtac attacagatt tatattctgg tgttacggga ttacattgca gcagagagag
      421 ttaaggacag tgttacaagt actacagaga atatcgctgc acagatactt ctatgtgata
      481 gacttctaca ggacttggtt acaaggtgt
//
LOCUS       DADSZD010000446          505 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.448, whole genome shotgun sequence.
ACCESSION   DADSZD010000446 DADSZD010000000
VERSION     DADSZD010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.448"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            5..454
                     /locus_tag="J1Z28_004655"
     CDS             5..454
                     /locus_tag="J1Z28_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HBA7719385.1"
                     /translation="MISTDDNFNHKFRTISRYRDFVNSPSVLNIIATIIAYNECEGWL
                     KSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFDEFYSRLIHEG
                     KVGIMAGHVYGTEGEGYLRLNLACGREKLYMGLTRLVSVIKNINQGE"
BASE COUNT          155 a           83 c          109 g          158 t
ORIGIN      
        1 gtatatgatt tcaactgacg ataattttaa tcataagttc agaacgatat cgaggtatcg
       61 cgattttgta aactccccat cagtactgaa tataattgca actattattg cctataacga
      121 atgtgaggga tggttaaagt cattaaaggc acatattgcc tctaacattc aattcaccca
      181 gcagtatctg aatgaaaata ttccagaatt gatcgtaagg ccagctgatg gttgctattt
      241 ttcctggata gattgttctg ctattggtta tccttttgat gaattttaca gtcgattaat
      301 tcatgaaggc aaagtgggca ttatggcggg gcatgtctat ggaacggagg gggagggcta
      361 ccttcgtctg aacctggcct gcggtcgaga aaaattatat atggggctta cgcgtcttgt
      421 gagtgtaatt aaaaatatta atcagggaga ataaaaatga ataacacgga tcttatccat
      481 ctcattaaac attttatgcg taacg
//
LOCUS       DADSZD010000447          501 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.449, whole genome shotgun sequence.
ACCESSION   DADSZD010000447 DADSZD010000000
VERSION     DADSZD010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.449"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>501
                     /locus_tag="J1Z28_004656"
     CDS             <1..>501
                     /locus_tag="J1Z28_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000077296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719386.1"
                     /translation="TELHAISEKPQNDDAAWLIEAESSIKFLKENAQNDEVVIYAIGS
                     SVLIHGALALEKSVTAADRWALQNMRMDVEYCWKIQKSWGGGQGHRIYLEPPLDGVFK
                     GGEALIYRRRFHGVEEGRTPIELSQKLVHSLGLYYLPERNAYCRLDDRGDIEDVIRII
                     NKNISSP"
BASE COUNT          154 a           78 c          126 g          143 t
ORIGIN      
        1 cacagaactt catgctattt cggaaaagcc acagaatgat gatgccgctt ggttaataga
       61 ggctgaaagt tccattaaat ttctgaaaga aaatgctcaa aatgatgaag tagtcatcta
      121 cgcgattgga agtagtgtcc taattcatgg cgctctagcc ctagagaaaa gtgttactgc
      181 agcagataga tgggcattgc agaatatgcg tatggatgtt gagtattgtt ggaaaattca
      241 aaagtcatgg ggtggaggtc aaggacatcg tatttatctt gagccacctt tagatggggt
      301 gttcaaaggg ggagaagcat taatttatcg tcgtaggttt catggtgttg aagaaggccg
      361 tacacctatc gaattgagtc agaaacttgt gcatagcctt gggttatatt acttgccaga
      421 aagaaatgca tattgtcgtc tcgatgatcg gggtgatatt gaggatgtaa tcaggataat
      481 taacaaaaac atttcatctc c
//
LOCUS       DADSZD010000448          500 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.450, whole genome shotgun sequence.
ACCESSION   DADSZD010000448 DADSZD010000000
VERSION     DADSZD010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.450"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          146 a          113 c          125 g          116 t
ORIGIN      
        1 cggcaccact taacaggcat tccgtaactt cccccagact tactatgacg cattatcggt
       61 gcactgaaaa caggagagtg cgcttaaaac cgcagaccgc atgcgggtgt tactgcatga
      121 cagtctggcc gtattgtgag atgtcggcag gaaccgatgt ccgcgcaggg gggaacttcc
      181 tgtagtacct gacgcttcaa atggtattaa ccgtagggcg ttgttggtgc ggaaatggga
      241 gcacaggtct gctacggggt gtacctctga cagtaaatcc gactcgctca tctgacagcc
      301 tctctcccgg aaaagaccaa aaatgagatc gtctcctcaa cagcagagaa gaggtcttga
      361 cagacaggct acattgcata cgatttatga agaaaaagac agcaaatatg gaggttatag
      421 cggagaacac cctacgaaat tcctatgaaa aacgattgaa aaaaatatca aattcgattc
      481 gtttttatat gctttttggg
//
LOCUS       DADSZD010000449          485 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.451, whole genome shotgun sequence.
ACCESSION   DADSZD010000449 DADSZD010000000
VERSION     DADSZD010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.451"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..319)
                     /locus_tag="J1Z28_004657"
     CDS             complement(<1..319)
                     /locus_tag="J1Z28_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HBA7719387.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLADAGVFR
                     WVTD"
BASE COUNT          130 a          103 c          103 g          149 t
ORIGIN      
        1 catcagtaac ccaccggaaa acgccagcgt ctgccagtgt tgaacagtat tcccgaaatg
       61 tagattttcc gcgaatatga agcaatgcgg cctcttttat tttagctggg ttcttggtcg
      121 tactaacttt taacaggttc ctggttcctc ttaataacaa aaccgtgtca tcgtgagtaa
      181 taattctgat gttatccgta gccagataat aaatgtaatg tgcaatacgg tgatgtttta
      241 attctgaata aaaccaggag aagttttgct cttttctcac ttgctcaaac atcttttgaa
      301 aaacaacgac ctgatccatc aggataataa cctcttgtta ggtgtgagac tgcgtagtgt
      361 gcacgatcgg ttttaccact tcaatctggt ctgtcctttg gctgtgatat gtacaaagtg
      421 tgaaaggggg attacctgaa ttctcccggt gggcattttg caacggacca gctccgctac
      481 aaacg
//
LOCUS       DADSZD010000450          481 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.452, whole genome shotgun sequence.
ACCESSION   DADSZD010000450 DADSZD010000000
VERSION     DADSZD010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.452"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            1..237
                     /locus_tag="J1Z28_004658"
     CDS             1..237
                     /locus_tag="J1Z28_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBA7719388.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     NAIADMARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            249..>481
                     /gene="rclC"
                     /locus_tag="J1Z28_004659"
     CDS             249..>481
                     /gene="rclC"
                     /locus_tag="J1Z28_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBA7719389.1"
                     /translation="MERYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQHLTHEGEYKPEA"
BASE COUNT          156 a           92 c           93 g          140 t
ORIGIN      
        1 atgtttaaaa aatctgtttt atttgcaaca ctattatctg gcgttatggc attttccacc
       61 aatgcagatg ataaaataat tctgaaacat atcagcgtct catcagtatc agcatcaccg
      121 acagttcttg agaatgccat tgctgatatg gccagaaaat ataatgcttc atcctggaaa
      181 gttacatcga tgcgaattga taataattca accgcaacag cagtattgta taaataagga
      241 tgtttacaat ggaaagatac ctgcacctgt taagtcgggg agataaaatt ggcctgacat
      301 tgattcgtct gagtattgcg attgttttta tgtggattgg gttattaaag tttgtccctt
      361 acgaggcaga cagcattaca ccattcgtcg caaacagtcc actaatgtcg ttcttttatg
      421 aacacccgga agactataaa cagcatctga ctcacgaagg cgaatacaaa ccagaagcaa
      481 g
//
LOCUS       DADSZD010000451          480 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.453, whole genome shotgun sequence.
ACCESSION   DADSZD010000451 DADSZD010000000
VERSION     DADSZD010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.453"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            33..418
                     /locus_tag="J1Z28_004660"
                     /pseudo
     CDS             33..418
                     /locus_tag="J1Z28_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          195 a          103 c           72 g          110 t
ORIGIN      
        1 tagcataaaa caaatgatga ataaggaatg ttatgtcaat tgactttacc ccaggtgtaa
       61 taaatacata taacggtgat atttataact gcacaacaaa taccgataat gttaaaacgc
      121 cagacacacc gaagtggcct tgtgataacc gggaagaaca acaacccatt aattccccat
      181 tctctggaga aggatacacc tctgaacaat ttgatttagc gcaacaacaa cgccaacaaa
      241 ttaatgcgtg ccatacgaac accacatata caaatgcaga ctactcaaag ttgaggctca
      301 acttgtaagt cttattaata acattgaaac gataagctcg acaccacttc cgcaaaagac
      361 acaatcaata cttaatcaga taaataacat tagacacgaa aagattaaaa acctctgaat
      421 atagattatg gaacaatgga tactcatgcc gcgcccaaaa attaactgag ctcaactaaa
//
LOCUS       DADSZD010000452          477 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.454, whole genome shotgun sequence.
ACCESSION   DADSZD010000452 DADSZD010000000
VERSION     DADSZD010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.454"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..192
                     /locus_tag="J1Z28_004661"
     CDS             <1..192
                     /locus_tag="J1Z28_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719390.1"
                     /translation="TKKYGLVYTEELGWIDLGHAQGQDARILKRKLEQEHFSTYYDEF
                     HDWYFPVDYHQEMGIRKKY"
BASE COUNT          150 a           71 c          102 g          154 t
ORIGIN      
        1 acgaaaaagt atggattagt ttataccgaa gagttaggtt ggatcgattt ggggcatgct
       61 cagggacaag atgccagaat attaaaaaga aaattggagc aagaacattt ttcaacatat
      121 tacgatgaat ttcatgattg gtattttcct gttgattatc atcaggaaat gggaattcgg
      181 aaaaaatatt aggagtagat cttacatttc ataccggtgt ttatacgaag gttatggttc
      241 gttcatgtct ttcacccact cttaaagcaa gagttgcctt aacgttaatg tatggaactg
      301 cgaagaggtt tgaagcatgg caaaactcat ttatttttaa ctggtatact gatagcggat
      361 ttagtgcaga agatttagtc tcagacttga ttggttttta tcgtgtattt ggaacaggcc
      421 ccgatccact tttattggca aaacctttat cttacacaaa agccttacaa atatggg
//
LOCUS       DADSZD010000453          468 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.455, whole genome shotgun sequence.
ACCESSION   DADSZD010000453 DADSZD010000000
VERSION     DADSZD010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.455"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(31..>468)
                     /locus_tag="J1Z28_004662"
     CDS             complement(31..>468)
                     /locus_tag="J1Z28_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719391.1"
                     /translation="IGLAGGLNLYQYAPNPLGWIDPLGLLRCGLTGNEVGNASNLPVI
                     RPGTKEWRQAVEAIGSGKNTNFRVASEKDAVDLISQGRGRMQRQGAPSDPYNEMGEPY
                     KKGYEVHPNESHTQNAPENDTPHVKWKDWESGKSSGGRGHVFF"
BASE COUNT          119 a          126 c           76 g          147 t
ORIGIN      
        1 aaacgccatt tttcaaaaaa atcttcatat tcagaaaaag acatgacctc ttcctccgct
       61 agattttccc gattcccaat ctttccattt gacatggggt gtgtcatttt caggcgcatt
      121 ctgcgtatgt gattcatttg gatgaacttc atatcctttc ttgtaaggtt cccccatctc
      181 attataggga tcgctaggag caccttgtct ttgcatccgt ccacggcctt gactaataag
      241 atctacagca tctttttcgc ttgctaccct aaagttagta tttttaccgc ttccgatagc
      301 ttcaacagcc tgcctccatt cttttgtgcc aggtcgtata acaggtaaat ttgatgcatt
      361 accgacttca ttaccagtca gaccacatct caataaacct aacggatcaa tccacccaag
      421 aggattaggc gcatactgat atagattcag tccccccgcc agccctat
//
LOCUS       DADSZD010000454          454 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.457, whole genome shotgun sequence.
ACCESSION   DADSZD010000454 DADSZD010000000
VERSION     DADSZD010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.457"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          137 a           97 c           86 g          134 t
ORIGIN      
        1 ggatcgccgg aaaggacccg ttaaaatgat aacgattatc aataatgcct gattccattc
       61 tctctggtga tcatgccgga cgggtgattc tgaatccgcc ctctcgcatg gctgtcagca
      121 tggataatgc gaaaatacaa aacatgatta attcactgta ctcaggttaa gcttcatggg
      181 tgcatcccat actgacatat gattaaactg caccatattg ctcttctcct gtactggtgc
      241 actgccgctt ttgtcatgtt ccctgaggga accttgagac actggacaga aaaataatct
      301 gtactgactt tactttttct gcactgattg tgttttctga catttagtga ccagagaaaa
      361 aatacggtat tgccattcag ttaacacgaa tattccgtgt tgattacaat aagtaaaata
      421 atataaaaac atggtcgcaa aaataaacca tctc
//
LOCUS       DADSZD010000455          450 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.458, whole genome shotgun sequence.
ACCESSION   DADSZD010000455 DADSZD010000000
VERSION     DADSZD010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.458"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..450
                     /locus_tag="J1Z28_004663"
     CDS             <1..450
                     /locus_tag="J1Z28_004663"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719392.1"
                     /translation="CYYHFAIFKQFNHRLSEACLHLQNAQYYCGIWKGARDRLDWITH
                     SEKKQIERTEKARKAGLKRGEKLFGAVKTEAIRLLKANMPKNGWIDPTEAFDCIDDEL
                     WFFIEEQTKKGEEPVLIYDELERTVLRWLAEDENVKAAFEEVIIGKQ"
BASE COUNT          154 a           69 c          113 g          114 t
ORIGIN      
        1 tgttattatc attttgctat atttaagcaa tttaaccatc gattgagcga agcatgtctg
       61 catctccaga atgcacagta ttattgcggg atatggaaag gtgcccggga tcggctggat
      121 tggattaccc atagcgaaaa aaaacaaata gaaagaactg aaaaggcaag aaaggcgggt
      181 ttaaaacgcg gtgagaagct atttggcgca gttaaaaccg aagcaatccg gcttctcaaa
      241 gccaacatgc ctaaaaatgg gtggattgat ccaactgaag cctttgattg tattgacgat
      301 gaattgtggt tctttattga ggaacaaaca aaaaaaggtg aagaaccagt actgatttat
      361 gatgaattag agcgcacagt gcttaggtgg ttagcagagg atgaaaatgt gaaagccgct
      421 tttgaagaag tgattattgg caaacagtga
//
LOCUS       DADSZD010000456          445 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.459, whole genome shotgun sequence.
ACCESSION   DADSZD010000456 DADSZD010000000
VERSION     DADSZD010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.459"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..414
                     /locus_tag="J1Z28_004664"
     CDS             <1..414
                     /locus_tag="J1Z28_004664"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719393.1"
                     /translation="AFFDGEFRCAEDHLFLFGFANEMELQRLYNVMGQYYFELKKVME
                     QIGERDNLDTFVAKAYQGGVRGLYTIAEGMVMFGREGEIWAIYLNDGEIRYFTNVHKW
                     KKTIPKTFEQWRERFPEIEINYGGDISTLPTQEDL"
BASE COUNT          140 a           61 c          109 g          135 t
ORIGIN      
        1 gccttttttg atggtgagtt tcgttgtgca gaggatcatt tgtttttatt tggttttgca
       61 aatgaaatgg aacttcagag attatacaac gttatggggc aatattactt tgaactgaaa
      121 aaagtgatgg agcaaatagg tgagcgcgat aatcttgata cttttgtcgc aaaggcttat
      181 cagggaggag ttagaggact ttataccatt gcggaaggta tggtgatgtt tggtagagag
      241 ggcgagatat gggctattta tctcaatgat ggtgaaattc gttatttcac aaatgtacat
      301 aaatggaaaa aaactattcc aaaaactttt gaacagtggc gagaacgatt tccagagatt
      361 gaaataaatt atggaggtga tatctcgaca cttcctacac aagaagattt atagacaaat
      421 gacccgcgat tcgccaacgc gggtt
//
LOCUS       DADSZD010000457          441 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.460, whole genome shotgun sequence.
ACCESSION   DADSZD010000457 DADSZD010000000
VERSION     DADSZD010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.460"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>441
                     /gene="ompT"
                     /locus_tag="J1Z28_004665"
     CDS             <1..>441
                     /gene="ompT"
                     /locus_tag="J1Z28_004665"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HBA7719394.1"
                     /translation="KLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGKTKE
                     RVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMVDQD
                     WMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGL"
BASE COUNT          132 a          103 c          101 g          105 t
ORIGIN      
        1 aaacttctgg gaatagtcct gacaacccct attgcgatca gctcttttgc ttctaccgag
       61 actttatcgt ttactcctga caacataaat gcggacatta gtcttggaac tctgagcgga
      121 aaaacaaaag agcgtgttta tctagccgaa gaaggaggcc gaaaagtcag tcaacttgac
      181 tggaaattca ataacgctgc aattattaaa ggtgcaatta attgggattt gatgccccag
      241 atatctatcg gggctgctgg ctggacaaca ctcggcagcc gaggtggcaa tatggtcgat
      301 caggactgga tggattccag taaccccgga acctggacgg atgaaagtag acaccctgat
      361 acacaactca attatgccaa cgaatttgat ctgaatatca aaggctggct cctcaacgaa
      421 cccaattacc gcctgggact c
//
LOCUS       DADSZD010000458          441 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.461, whole genome shotgun sequence.
ACCESSION   DADSZD010000458 DADSZD010000000
VERSION     DADSZD010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.461"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            246..401
                     /locus_tag="J1Z28_004666"
                     /pseudo
     CDS             246..401
                     /locus_tag="J1Z28_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          123 a          110 c           98 g          110 t
ORIGIN      
        1 ggaagaataa gtggttgcag tttcagactg tccgccggta caagttcgct cagaataacc
       61 aacctgagtt gcaatatcga caatgtactc gccctgctag cgcatttgct ttaccatcta
      121 aaagtcttct tgctcagtat gttgatgtcc tttctgatgt gagaacctct aggaaacaac
      181 atgttggatg gttcgagaca atccaaatgg tgatttaccg tcttatatca ttggcgctga
      241 caccccggcg ttccagttcg tcaatacgag gagaacatcc gtcaaggcgg ggtaataaag
      301 ctctcaagcg gtcattgttc ctgacagcct tcgctgcact catagcccca atatccaagg
      361 cctactacac acacaaaatg aggcagggaa aacgacataa ctaggtgttt atcgccctag
      421 caagacaacg cagagacgtt c
//
LOCUS       DADSZD010000459          438 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.462, whole genome shotgun sequence.
ACCESSION   DADSZD010000459 DADSZD010000000
VERSION     DADSZD010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.462"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>438
                     /locus_tag="J1Z28_004667"
     CDS             <1..>438
                     /locus_tag="J1Z28_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719395.1"
                     /translation="SLAGGINLYQYAPNPLSWIDPLGLTVTPLNKEGFYVYGLYKPGA
                     TEPYYVGHTEQNPLKREGQHAGTGRLGDAELRILKGEDGKLTYSQAKGYEQAYREKYK
                     TKTGFPGNVIEPIDKSRTDSRGRSHYRNYRAAAREIGIKPTKSR"
BASE COUNT          150 a           71 c          106 g          111 t
ORIGIN      
        1 agtttagctg gcgggataaa tctttatcag tatgcgccga atccgttgtc atggatagat
       61 cctttaggat taacggttac tcctctaaac aaagagggat tttatgttta tgggctttat
      121 aagccagggg ctacagagcc atattatgta ggtcatactg agcaaaaccc gttgaagcga
      181 gaaggtcaac atgctggaac aggaaggctt ggtgatgcag aattgcgaat acttaaaggt
      241 gaggatggta aactgacgta ttctcaggca aaaggctatg aacaagctta tcgagaaaaa
      301 tataaaacaa aaacaggttt tccaggaaat gttattgagc caatagataa gagtcgcact
      361 gattcacgag gtagatctca ttatagaaac tatcgtgcag cagctagaga aattggtata
      421 aaacctacca agagtaga
//
LOCUS       DADSZD010000460          435 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.463, whole genome shotgun sequence.
ACCESSION   DADSZD010000460 DADSZD010000000
VERSION     DADSZD010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.463"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          136 a           84 c           90 g          125 t
ORIGIN      
        1 agcagttcaa cagtttctgt tcaatggtct acctccttca caattaagtt ttctgtacac
       61 aaaaacaaag aagaagtaac cttatttatc cagaaataat gggcgtatga ctagatgagc
      121 agacatttag tcagaggtga cttacgtgat aaaaactgta tcgatcaggt aaataatcga
      181 aaacagtatt cgtctaaata tcgcagagac tggtttacga ggtgtcaaag cacacgtttg
      241 gattgcggca tccttgcttg tccgtaaggc ttttaccggg tcacagttca ccatgccgga
      301 ctgatgatct acacgcttag agttacattc caaagtaaaa ccatagatgg atgcaaacct
      361 gcatcgtcag gaggttaaat ttcagacctg tcagttaccg cgtaaatggt ttgatatatg
      421 actaatttat ttgcg
//
LOCUS       DADSZD010000461          428 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.464, whole genome shotgun sequence.
ACCESSION   DADSZD010000461 DADSZD010000000
VERSION     DADSZD010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.464"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..281)
                     /locus_tag="J1Z28_004668"
     CDS             complement(<1..281)
                     /locus_tag="J1Z28_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HBA7719396.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDHYPHEVFS
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMG"
BASE COUNT          133 a          107 c           83 g          105 t
ORIGIN      
        1 cccatagttt ccatattttc tggaagacgg aaaaagtgct tcattccatt acggaaataa
       61 cgtaaacgct gggcacgggt atatttgctc tcaccgccac caagaccatc tactacggct
      121 ttagaaacca gcataccatc gtaaggatga gaaaaaacct catgcggata atgatcgtca
      181 cgttgacgca cgcgatgttc gttacgcgtt tcgcggaaga agtcgaaact gggatcaata
      241 aaatcctgac tatctgggaa aaaatacttt agcttcgaca acttactctc ctgtgacacc
      301 actgcaacgt ctcaaaaaac gagggattct accatgaaat cccagcaagt agcattaagg
      361 tttagcctct ctaagggcct taaactcagc ccgaaaacgt ttttcctcaa atgaatttcc
      421 cgagtcac
//
LOCUS       DADSZD010000462          412 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.465, whole genome shotgun sequence.
ACCESSION   DADSZD010000462 DADSZD010000000
VERSION     DADSZD010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.465"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          113 a           83 c          104 g          112 t
ORIGIN      
        1 agaggcgggc tgaacttata tgcttatgca ccaaaccctc ttggttggat cgaccctctg
       61 ggattaaatg ggcattgtgc tttcggaaac aagagtaaac ctcgcgcacc aagaccggga
      121 aaagatattg atgttgatgc taatgggatg gtgaagtctc aggctggcga gttattacct
      181 cagggagcgt ctactacatt agatcccaat aaatcaccgt taagcgggca ttaccataca
      241 ataccagaag gaagtccatt gcctgatggt ttgggtatta aacttgacgg tagggatgta
      301 atccctggct ctccacatgc ggcaggtcat gctactattt atcctacacg agatatgtca
      361 atgactgaat ttcagaggtt gtttgattct ataccttggg aatatggtgg aa
//
LOCUS       DADSZD010000463          403 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.470, whole genome shotgun sequence.
ACCESSION   DADSZD010000463 DADSZD010000000
VERSION     DADSZD010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.470"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>403)
                     /locus_tag="J1Z28_004669"
     CDS             complement(<1..>403)
                     /locus_tag="J1Z28_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HBA7719397.1"
                     /translation="VYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAKYSAPNKT
                     DQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFASFSDASN
                     TMAHGRYYYGDHTNGIAWRLISQGEMYLTDN"
BASE COUNT          120 a           82 c           81 g          120 t
ORIGIN      
        1 atattgtcag taagatacat ctcgccctga gagattaaac gccaggcgat cccattggta
       61 tggtcaccat agtaatagcg accatgcgcc atcgtgttac tggcatctga gaaactggca
      121 aaactgctgg cataggaatt attggcaacc tgtaacgtaa attcattaaa cgtatcgcgt
      181 tgcaggtttt gccgtaaaac agaagtcagc atccagctat ctttcatttc aaaataactg
      241 tcgtcatttt cattatcttg ttgttgatcc gttttatttg gtgcggaata tttagccatt
      301 aatgataatg ttgcatcatc ccataacggg atattgcgat aacgaacatc cacagaattg
      361 gtattcatct gactggtacg cgtaaaatca cgagagtaaa cat
//
LOCUS       DADSZD010000464          395 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.472, whole genome shotgun sequence.
ACCESSION   DADSZD010000464 DADSZD010000000
VERSION     DADSZD010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.472"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>395
                     /locus_tag="J1Z28_004670"
     CDS             <1..>395
                     /locus_tag="J1Z28_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HBA7719398.1"
                     /translation="LTKQTGTGNRFVLQNLGNTELSFAVKVWGSGDRQNVFEVGTSAA
                     YLFYAQKTSSGQLFDVNGAINCTTLNQLSDRELKDNIQIISDATEAIRKMNGYTYTLK
                     ENGLPYAGVIAQEAMEAIPEAVGSFTHYG"
BASE COUNT          121 a           80 c           91 g          103 t
ORIGIN      
        1 ctcttacaaa acaaacaggg actggcaatc gttttgtttt acagaactta ggtaatacag
       61 aactatcatt tgctgtcaag gtgtggggtt caggtgatcg acaaaacgtt tttgaggttg
      121 gaacgtctgc cgcgtatctg ttttatgctc aaaaaacatc gtcaggtcag ctgtttgatg
      181 taaacggtgc tattaactgt acaacactga atcagttatc agaccgcgag ctgaaagaca
      241 atattcagat tatcagtgac gcaaccgaag ctatccgtaa aatgaacggg tacacctaca
      301 cacttaagga aaacggacta ccttacgctg gtgttattgc acaggaagca atggaggcaa
      361 taccggaagc ggtaggctca ttcactcatt acggt
//
LOCUS       DADSZD010000465          378 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.475, whole genome shotgun sequence.
ACCESSION   DADSZD010000465 DADSZD010000000
VERSION     DADSZD010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.475"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          127 a           56 c           92 g          103 t
ORIGIN      
        1 aacaaagtat tatatataag cgatagcaat attaagacct ctgaaagcgt atctgacaaa
       61 gaaaatggag ggataatagc cagcgcagta gtcagtgaat ttggttgcac catcgttatg
      121 aatggtgata attcagtcga gacgaggggg catattcagc gggactttta agtcaggtta
      181 atgattctga gactgtggaa aataacacca gtcttgaaac cacagataaa acgaacattg
      241 ttacctatgg gaaaaatgca gtaggagttc ttgcatgttc aagtcctgga gagtctcgaa
      301 catgtgtcga tgctgtagat gatgaagttt gtgattctaa cagttacgaa gttattagcc
      361 gtgctgattt aaaaatga
//
LOCUS       DADSZD010000466          377 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.476, whole genome shotgun sequence.
ACCESSION   DADSZD010000466 DADSZD010000000
VERSION     DADSZD010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.476"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT           95 a           56 c           90 g          136 t
ORIGIN      
        1 ttcctggtca tgcgtaatga ttaaatgctt ttgtgacacc attcttccgg tctgaatatt
       61 tatcccgttc ttttttattt gtgcagtgat actgtcggaa gagaattcag taatgtgagg
      121 tgtcctggtc aggataatat ctgctgagat attattagtt gagtatgttt ttactgagca
      181 aaagctggct tttaattcct gtactgaaac gctgtcgctc gtccggggtt aactgaaact
      241 ggatgatgat gtaaaatgaa ggctgttttt gtgtttgtcg aagaagtgtt attttaatcc
      301 attctcatca ttggctggcg attatacaga agtatttgaa gaaaaaatgg tgcattttat
      361 ctgcgtcagc agggagg
//
LOCUS       DADSZD010000467          376 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.477, whole genome shotgun sequence.
ACCESSION   DADSZD010000467 DADSZD010000000
VERSION     DADSZD010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.477"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>376)
                     /locus_tag="J1Z28_004671"
     CDS             complement(<1..>376)
                     /locus_tag="J1Z28_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HBA7719399.1"
                     /translation="AIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAM"
BASE COUNT          107 a           89 c           84 g           96 t
ORIGIN      
        1 ccattgctga cgggcgttta tggtgacttg tctgtgccac tttacaggtt gggaagcggg
       61 ttaaaaggaa caacaccatg accacaacca gaatcataat catatactta tacggttcaa
      121 gggtgttctc taacatcagc accttaaagt tgtgaatttg cccggcgttc attccggaca
      181 tctgcttctc aaggctttcc ccctcggaga aaaccagata tttgcccaat aaaataccag
      241 acgcagcacc aatcggataa aaggtctggc tgatattgag ccgcaatgtg gcataggctt
      301 ttggaccgat cattgaactg tatgtgttcg ctgcagtttc aaggaaactc aggccaatcg
      361 caatcgcaaa aatagc
//
LOCUS       DADSZD010000468          370 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.478, whole genome shotgun sequence.
ACCESSION   DADSZD010000468 DADSZD010000000
VERSION     DADSZD010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.478"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>370)
                     /locus_tag="J1Z28_004672"
     CDS             complement(<1..>370)
                     /locus_tag="J1Z28_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBA7719400.1"
                     /translation="NILIKAFVSSGQFLLPLIISLLVWAELWFGWSFMIAAGIMFINA
                     LFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGYISMATFYLVSQWLAQ
                     YGQFVAGMSYTMSIKLLSIYT"
BASE COUNT          124 a           70 c           87 g           89 t
ORIGIN      
        1 ggtgtagata ctgagtaatt tgatcgacat ggtgtatgac atgccagcga caaattgtcc
       61 gtattgtgcc agccactggc taaccagata aaacgttgcc attgagatat agccatatag
      121 ggaataactg gctaaatcga taattgaaca gcgatgttcc gtagagctgg tggttttctt
      181 tatgacaggt aagtgacgac ccggatgggg tgggaacgta caacggtata aaaacagagc
      241 gttaataaac ataatgcctg cggcaatcat aaaggaccaa ccgaaccaca gttcagccca
      301 cactaacagg ctgataatta gcggtaataa aaactgtccg ctggaaacaa atgctttaat
      361 taaaatattg
//
LOCUS       DADSZD010000469          369 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.480, whole genome shotgun sequence.
ACCESSION   DADSZD010000469 DADSZD010000000
VERSION     DADSZD010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.480"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
BASE COUNT          103 a           74 c           86 g          106 t
ORIGIN      
        1 ttaattctca gcacaggtgt tgactaaaga gcgtgaaact ttgctattat gtcttcgtaa
       61 gattcacgga cggttatact tgagcctgat tctgtgaagt aaacaacagc agaagcatcg
      121 ttgccttttt caatgtatga aacattccag tcatggatag ccactgcggg ctgaccatta
      181 tcccgacggt gcgtcttaat gaatcgcgga agtaattctg caatatcgtt aaaaacacca
      241 tttacggtat gagtgatacc accaacgcaa tgtagatgag ttgactccgg ggtatcattg
      301 tctgcttctg caaagagtat agctgtcttg ctaattgtaa caggcgcctg tgagcgggat
      361 aattcgaga
//
LOCUS       DADSZD010000470          360 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.482, whole genome shotgun sequence.
ACCESSION   DADSZD010000470 DADSZD010000000
VERSION     DADSZD010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.482"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(<1..>360)
                     /locus_tag="J1Z28_004673"
     CDS             complement(<1..>360)
                     /locus_tag="J1Z28_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4034 domain-containing protein"
                     /protein_id="HBA7719401.1"
                     /translation="QAEKRYFLSWTQMCNYFYDMNTLVDAGTEGLRLIKTWQQARPHS
                     THAWLAEAQYWNHRAWLYRSYGWANDTTHAMWLCAGACNEQMVIATLKAIDCDPRQWM
                     AALLTSTNSKVFGQPAWL"
BASE COUNT           94 a           78 c           82 g          106 t
ORIGIN      
        1 agccaggctg gctgaccaaa tacttttgag ttggtgctgg taagtaatgc agccatccat
       61 tgtcgtgggt cacaatcaat tgctttaagt gtagcgatga ccatttgttc attacatgca
      121 ccagcacaaa gccacattgc atgtgtggta tcatttgccc agccataact gcgatataac
      181 catgcacggt gattccagta ttgtgcttcg gcaagccagg catgtgtaga atgaggtctt
      241 gcttgctgcc aggttttaat gagtcttaaa ccttctgttc cggcatcaac tagcgtgttc
      301 atatcataaa aatagttgca catttgggtc caggataaaa aatagcgctt ttcagcctga
//
LOCUS       DADSZD010000471          356 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.483, whole genome shotgun sequence.
ACCESSION   DADSZD010000471 DADSZD010000000
VERSION     DADSZD010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.483"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>356
                     /locus_tag="J1Z28_004674"
     CDS             <1..>356
                     /locus_tag="J1Z28_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719402.1"
                     /translation="KKTASGAITWSVIVTQILSPVSLSLIPANSFASSGNKDVTQTYA
                     SDEHANKVASFAASAGQSLANNNASSFAVNTLSTQATKEVVDWLQQYGNARIKLNVDD
                     SFSLKDSSFDFLYPWL"
BASE COUNT          103 a           72 c           74 g          107 t
ORIGIN      
        1 aaaaaaactg catctggcgc gattacatgg tcggtaattg ttacccagat attatctcca
       61 gtttctcttt ctttgatccc agcaaacagt tttgcatcat ctggtaataa agatgttacg
      121 caaacttatg ccagcgatga gcatgcaaat aaagtggcct catttgcagc aagtgcaggt
      181 cagagcctgg cgaataataa tgcaagtagt tttgctgtaa atactttatc aactcaggcg
      241 acaaaagagg tcgtcgactg gttgcaacaa tatggcaatg cgcgcatcaa gcttaatgtc
      301 gatgattctt tttccttgaa ggattcatca ttcgactttt tatatccatg gctgga
//
LOCUS       DADSZD010000472          355 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.484, whole genome shotgun sequence.
ACCESSION   DADSZD010000472 DADSZD010000000
VERSION     DADSZD010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.484"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            48..124
                     /locus_tag="J1Z28_004675"
     tRNA            48..124
                     /locus_tag="J1Z28_004675"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Ile,seq:gat)
     gene            167..242
                     /locus_tag="J1Z28_004676"
     tRNA            167..242
                     /locus_tag="J1Z28_004676"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:200..202,aa:Ala,seq:tgc)
BASE COUNT          102 a           77 c           86 g           90 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaatg agcagtaaaa cctctacagg cttgtagctc
       61 aggtggttag agcgcacccc tgataagggt gaggtcggtg gttcaagtcc actcaggcct
      121 accaaatttg caccgcaaat ttgaagaggt tttaactaca tgttatgggg ctatagctca
      181 gctgggagag cgcctgcttt gcacgcagga ggtctgcggt tcgatcccgc atagctccac
      241 catctctgta gtgattaaat aaaaaatact tcagagtgta cctgcaaagg ttcactgcga
      301 agttttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac aacga
//
LOCUS       DADSZD010000473          352 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.485, whole genome shotgun sequence.
ACCESSION   DADSZD010000473 DADSZD010000000
VERSION     DADSZD010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.485"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..233
                     /gene="uraH"
                     /locus_tag="J1Z28_004677"
     CDS             <1..233
                     /gene="uraH"
                     /locus_tag="J1Z28_004677"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBA7719403.1"
                     /translation="TAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFP
                     EIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
BASE COUNT          113 a           67 c           77 g           95 t
ORIGIN      
        1 ataccgccaa aacagataag gatggacgaa ttaaggcact gtggcccgag caaactgcga
       61 ctactggcga ttaccgtgtc gtatttaaaa ccggggacta tttcaagaaa caaaatcttg
      121 aaagtttctt ccctgagatt cccgttgaat ttcatattaa taaagtgaac gagcattatc
      181 atgtgccttt gttactcagc caatatgggt attcaaccta tcgtggcagt taatgcgtaa
      241 atatgtaaaa gctgatctct gctactgtta gggaggtgac tccctgacag attaacagta
      301 aacggctctt gttggctaac gacaaaaaag tgtgatggct tatgaaaaag aa
//
LOCUS       DADSZD010000474          348 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.486, whole genome shotgun sequence.
ACCESSION   DADSZD010000474 DADSZD010000000
VERSION     DADSZD010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.486"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>348
                     /locus_tag="J1Z28_004678"
     CDS             <1..>348
                     /locus_tag="J1Z28_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBA7719404.1"
                     /translation="SAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQ
                     KSAATSASTATTKASEAATSARDAAASKEAAKSSETSAASSASNAASSATAAGNSAKA
                     AKTSETNADNSAQ"
BASE COUNT          103 a          106 c           98 g           41 t
ORIGIN      
        1 ccagcgcagg aacggcatca acaaaggcta ctgaagcatc aaaaagtgct gccgctgcag
       61 agtcctcaaa aagcgcggcg gctaccagtg ccggtgcggc gaaaacgtca gaaacgaatg
      121 ctgcagcgtc acaaaaatct gcagccactt ctgcatccac cgcgaccacg aaagcgtcag
      181 aagctgccac ctcagcccgg gatgcggcgg cctcaaaaga ggcagcgaaa tcatcagaaa
      241 cgagcgcagc ctcgagcgcc agtaatgccg cttcctcggc aacggcggca ggaaattccg
      301 cgaaggccgc aaaaacgtct gagacgaatg cggataacag cgcacagg
//
LOCUS       DADSZD010000475          329 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.490, whole genome shotgun sequence.
ACCESSION   DADSZD010000475 DADSZD010000000
VERSION     DADSZD010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.490"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>329
                     /locus_tag="J1Z28_004679"
     CDS             <1..>329
                     /locus_tag="J1Z28_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBA7719405.1"
                     /translation="DIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHK
                     ESEKPFWNKPYTILEKDGIKIGIVGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSAT
                     LKNQGIE"
BASE COUNT           99 a           69 c           70 g           91 t
ORIGIN      
        1 ttgatattat gaataccatg ccttttgacg cagtatcaat cggtaatcat gagtttgacc
       61 atggctggga taatgccctc cgacaattaa gcaaagcgaa tttccctgtt ttattaggca
      121 atgtttatca taaagagagt gagaaaccct tctggaacaa gccctacacc atcctggaaa
      181 aagatggcat caagattggt attgttgggc tacacggcgt atttgcattt aatgacacgg
      241 tctcggagct ctctctccag ggactcgata atgacaataa taaccgattt gataaatctt
      301 ccgcgaccct caagaatcag gggattgaa
//
LOCUS       DADSZD010000476          322 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.491, whole genome shotgun sequence.
ACCESSION   DADSZD010000476 DADSZD010000000
VERSION     DADSZD010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.491"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            5..214
                     /gene="fumD"
                     /locus_tag="J1Z28_004680"
     CDS             5..214
                     /gene="fumD"
                     /locus_tag="J1Z28_004680"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBA7719406.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          105 a           56 c           84 g           77 t
ORIGIN      
        1 aagaatggga aaccggacaa aagaggatga actgtaccga gaaatgtgca gagttgtcgg
       61 taaagttgtg ctggaaatgc gcgatctggg gcaggaacca aagcatattg ttatcgcagg
      121 cgtattacgt acagcattag ccaataaacg cattcagcgc agtgagctgg aaaagcaggc
      181 gatggaaaca gtcattaacg cactggtaaa atgaccattc aggatgaggc ggtaagcttc
      241 atcctgatat tgttagttca gttgcggtcc agttaatcat tctaaataac aactgctgaa
      301 attatacgaa tttcggtgta ta
//
LOCUS       DADSZD010000477          319 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.492, whole genome shotgun sequence.
ACCESSION   DADSZD010000477 DADSZD010000000
VERSION     DADSZD010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.492"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            163..>319
                     /locus_tag="J1Z28_004681"
     CDS             163..>319
                     /locus_tag="J1Z28_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBA7719407.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKG"
BASE COUNT           98 a           63 c           60 g           98 t
ORIGIN      
        1 ctctggtttt ctttcttacc tgaacaatct ggttatagcg tgaggactga ccattaattt
       61 tctccatctt actggataac acactatgag ctgctacctt taaaacacct gaatgtcaac
      121 aggttaactc gtgccatatc gttataatcc ctttgatatt agatacaaat taaggtcata
      181 tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc tgtaatagat
      241 aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag ttcgatgaat
      301 gaaagcacgg cgaagggaa
//
LOCUS       DADSZD010000478          305 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.494, whole genome shotgun sequence.
ACCESSION   DADSZD010000478 DADSZD010000000
VERSION     DADSZD010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.494"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>305
                     /locus_tag="J1Z28_004682"
     CDS             <1..>305
                     /locus_tag="J1Z28_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein"
                     /protein_id="HBA7719408.1"
                     /translation="QGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQF
                     EFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKSAFV"
BASE COUNT           63 a           79 c           88 g           75 t
ORIGIN      
        1 cagggcgatc cggcatcggt atcgttccgg attgccgcac cggcagcgcc gtcgcggatt
       61 gagctgacgc cgggctattt tcagataacc gccacgccgc atcttgcggt ttatgatccg
      121 acggtacagt ttgagttctg gttctcggaa aaacggattg cggatatcag gcaggttgaa
      181 accacagccc gctatcttgg cacggcgctg tactggatag ctgccagtat caatatcagg
      241 ccgggccatg attattattt ttacgttcgc agtgtgaaca ccgttggcaa atcggcattc
      301 gtgga
//
LOCUS       DADSZD010000479          305 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.495, whole genome shotgun sequence.
ACCESSION   DADSZD010000479 DADSZD010000000
VERSION     DADSZD010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.495"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>305
                     /locus_tag="J1Z28_004683"
     CDS             <1..>305
                     /locus_tag="J1Z28_004683"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335133.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate from
                     phosphoenolpyruvate and D-erythrose-4-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBA7719409.1"
                     /translation="GGREPNYGLSDITKAVKLMHDEGINHRLIIDCSHGNSGKVAKRQ
                     ISVARQVINNRKKIPGYVAGIMVESFLQDGKQSDSFPLEYGQSVTDECISWQQTEQL"
BASE COUNT          102 a           45 c           72 g           86 t
ORIGIN      
        1 gtggaggtag agaacctaac tatggtttgt ctgatataac taaggctgtg aaattaatgc
       61 atgatgaggg cattaaccat cgtctgataa ttgattgtag ccatggtaat agcggtaaag
      121 tggctaaacg gcaaatttcc gttgcacgtc aagttataaa taatcggaaa aaaataccag
      181 gatatgtagc tggcattatg gtggaaagct ttttacaaga tggtaaacaa tccgatagtt
      241 ttccgctaga gtatggtcag tctgttactg atgaatgcat atcatggcaa cagacagaac
      301 aatta
//
LOCUS       DADSZD010000480          300 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.denovo.497, whole genome shotgun sequence.
ACCESSION   DADSZD010000480 DADSZD010000000
VERSION     DADSZD010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.denovo.497"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            <1..>300
                     /locus_tag="J1Z28_004684"
     CDS             <1..>300
                     /locus_tag="J1Z28_004684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBA7719410.1"
                     /translation="KAEADDAAAQADKDGNGLITPEEAKAVEDANVALEAAKQAAQEA
                     VNKVPDADKGNLQDRVDALTPAQVPDVTDANGNGKADTAEQAVADAEAAVKAAEAK"
BASE COUNT           84 a           71 c          114 g           31 t
ORIGIN      
        1 aaagcagaag cagacgatgc tgccgcgcag gctgataagg acggtaacgg cctgatcacg
       61 ccggaagagg cgaaagccgt ggaagatgcg aatgtggcgc tggaagcagc gaagcaggca
      121 gctcaggaag cggtgaacaa ggtaccggat gcagacaagg gtaacctgca ggatcgcgtg
      181 gatgcgctga cgccggctca ggtaccggac gtgacggatg cgaacggtaa cggcaaggca
      241 gacacagccg agcaggcagt ggccgatgcg gaagctgcag tcaaggcagc ggaagcgaag
//
LOCUS       DADSZD010000481        17378 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.guided.094, whole genome shotgun sequence.
ACCESSION   DADSZD010000481 DADSZD010000000
VERSION     DADSZD010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.guided.094"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="J1Z28_004685"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="J1Z28_004685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBA7719411.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="J1Z28_004686"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="J1Z28_004686"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBA7719412.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMKFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="psd"
                     /locus_tag="J1Z28_004687"
     CDS             2040..3008
                     /gene="psd"
                     /locus_tag="J1Z28_004687"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBA7719413.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            3030..6353
                     /gene="mscM"
                     /locus_tag="J1Z28_004688"
     CDS             3030..6353
                     /gene="mscM"
                     /locus_tag="J1Z28_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBA7719414.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLIGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKDGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6503..8005)
                     /gene="yjeM"
                     /locus_tag="J1Z28_004689"
     CDS             complement(6503..8005)
                     /gene="yjeM"
                     /locus_tag="J1Z28_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBA7719415.1"
                     /translation="MPHSIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWHIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8224..9201)
                     /gene="epmA"
                     /locus_tag="J1Z28_004690"
     CDS             complement(8224..9201)
                     /gene="epmA"
                     /locus_tag="J1Z28_004690"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBA7719416.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9526..11334
                     /gene="frdA"
                     /locus_tag="J1Z28_004691"
     CDS             9526..11334
                     /gene="frdA"
                     /locus_tag="J1Z28_004691"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBA7719417.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11327..12061
                     /locus_tag="J1Z28_004692"
     CDS             11327..12061
                     /locus_tag="J1Z28_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HBA7719418.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNARTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12072..12467
                     /gene="frdC"
                     /locus_tag="J1Z28_004693"
     CDS             12072..12467
                     /gene="frdC"
                     /locus_tag="J1Z28_004693"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBA7719419.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12478..12837
                     /gene="frdD"
                     /locus_tag="J1Z28_004694"
     CDS             12478..12837
                     /gene="frdD"
                     /locus_tag="J1Z28_004694"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBA7719420.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12900..14033
                     /gene="blaEC"
                     /locus_tag="J1Z28_004695"
     CDS             12900..14033
                     /gene="blaEC"
                     /locus_tag="J1Z28_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBA7719421.1"
                     /translation="MFKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYAGIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSADEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPFDINEKILQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVTAAWQILNTLQ"
     gene            14122..14655
                     /locus_tag="J1Z28_004696"
     CDS             14122..14655
                     /locus_tag="J1Z28_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HBA7719422.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14652..14969)
                     /locus_tag="J1Z28_004697"
     CDS             complement(14652..14969)
                     /locus_tag="J1Z28_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound-resistance protein
                     SugE"
                     /protein_id="HBA7719423.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSLA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="J1Z28_004698"
     CDS             complement(15152..15298)
                     /gene="ecnB"
                     /locus_tag="J1Z28_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBA7719424.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="J1Z28_004699"
     CDS             complement(15409..15534)
                     /gene="ecnA"
                     /locus_tag="J1Z28_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBA7719425.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(15586..16152)
                     /gene="efp"
                     /locus_tag="J1Z28_004700"
     CDS             complement(15586..16152)
                     /gene="efp"
                     /locus_tag="J1Z28_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBA7719426.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16194..17222
                     /gene="epmB"
                     /locus_tag="J1Z28_004701"
     CDS             16194..17222
                     /gene="epmB"
                     /locus_tag="J1Z28_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBA7719427.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPNDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4331 a         4555 c         4467 g         4025 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgtatt atacgagaaa tctttccctc
      121 tgcaaccccc ctaaaaaatt ttttttgagt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccaactcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaagccgtca agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctaacatc gagatagcgg tagtggaagt
      421 aggcttccag ctccggcatg tatttaaaca ggaagcgacg gtcctgaccg atgctgttac
      481 cacaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcacg atcgcccatc gtgctcgctt tcacacgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcaggt tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtctgtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtactatc cgttcgctgg tgaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cttcagtgtt aacgcgcccg gacttctacg atggcgtgaa acctattgcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat tgtgctcaac aaaatagacc
     1381 tgctggacga cgaaggtatg aagttcgtca acgagcagat ggatatctac cgcaatatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg acgtatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgct gctggggctg caaaaagaga tcctgaccaa cgatgtatct gacaactcgg
     1621 gtctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgactc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 cccagggctt tgtcgaattc catgactact taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgtagg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggttatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggatctc ttccgtaacg gtacgtttgt gaccacttac ctctccccac
     2461 gtgactacca ccgcgtacac atgccgtgta acggtattct gcgtgaaatg atctacgtgc
     2521 cgggcgatct cttctccgtt aaccatctca cggctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatctgc ctgttcgata ccgaatttgg cccgatggcg cagattctgg
     2641 tcggtgcgac gattgtaggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggcat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcat
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aacttggctc taccgttatc aacctgtttg
     2821 caccaggtaa agtgaacctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaacca gccccgcttc
     2941 ctgctgaaga aatcgaggca gaacacgacg ccagcccatt ggttgacgac aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcacccagg
     3121 aactggagca ggcaaaagcg gcgaaacctg cgcagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaag caatatcaag
     3241 aagtcattga taattatccg aaactttccg ccactctgcg cgcgcagtta aacaacatgc
     3301 gtgacgagcc acgcagcgtg tcgccgggaa tgtctaacga tgcgctgaat caggaaattc
     3361 tccagatcag cagtcagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgat gcccgccgtc
     3481 agttgaatga aatcgagcgc cgcctgggga cacttatcgg caataccccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactccg cacgtctgaa agcgctcgtt gatgaactgg
     3601 agctggcgca gctatctgcc aataaccgcc aggaattagc gcgcttgcgc tctgagctgg
     3661 cggaaaaaga gagccagcag ctggatgcat atttgcaagc actacgcaac cagttgaata
     3721 gtcaacgcca actggaagcg gaacgggcgc tggaaagtac cgagcaactg gcggaaagta
     3781 gcgccgattt gccaaaagat atcgtcgccc agtttaaaat taaccgcgaa ctatcggcgg
     3841 cgctgaatca acaggcgcag cggatggatc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcatctaa cctgctcggc gaagcgttgc gggcgcaggt ggcacggcta ccggaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttacg tgtgcaacgg ttacgttatg
     4081 aggatctgct taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccac
     4141 tgaccgctga gcaaaaccgt attctggaag cacagctacg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tgctggagct gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc gacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagtaaaga gacgattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttacgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgctgcctgt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggtgat ggcgtaacag ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgctcgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgttactacc tgatgagcat cgggcttatt gtgccgctga
     4861 ttatggcgct gatgatgttc gataacctcg acgaccgcga attttccggt tcgctgggac
     4921 ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt caccctcagc ctgaaaaagg
     4981 ccgggatccc gctgtatctc aacaaagatg gcagcggcga caacattacc aaccatatgc
     5041 tgtggaacat gatgattggc gcacctctgg ttgccattct ggcgtcggcg gtgggttatc
     5101 tggcaacggc gcaggcgctg ttagcgaggc ttgaaacctc ggttgccatc tggttcctgc
     5161 tactggtggt ttatcacgtt atccgccgct ggatgctgat ccagcgccgc aggctggcgt
     5221 ttgaccgggc aaagcatcgc cgggcagaga tgttagcgca gcgtgcgcgt ggcgaagagg
     5281 aagcgcatca tcacagtagc ccggaaggag caattgaagt cgatgaaagc gaagtcgatc
     5341 tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc aattttgatg ttgatcgccc
     5401 tgctttcggt cattgtgctg tggtcagaaa tccattccgc tttcggcttc ctcgaaaata
     5461 tttcgctgtg ggatgtcact tccacggtac agggcgtaga aagcctggag ccgattaccc
     5521 tcggtgcggt actgattgcc attctggtgt ttatcatcac cacgcagctg gtgcgcaacc
     5581 tgcccgcgct gctggaactg gcgattctgc agcacctgga tttaacgccg ggtacgggct
     5641 acgccatcac caccatcacc aaatatctgc tgatgctgat tggcgggctg gtcggcttct
     5701 cgatgattgg tattgagtgg tcgaaattgc agtggctggt cgccgcgctc ggtgttggtc
     5761 tcggttttgg tttgcaggaa attttcgcca actttatctc tggcctgatt atcctgttcg
     5821 aaaaaccgat tcgcattggc gatacggtaa cgatccgcga tcttaccggt agtgtgacaa
     5881 aaattaacac ccgcgccacc accatcagcg actgggaccg caaagaaatt atcgtgccga
     5941 acaaggcgtt tattaccgag cagtttatca actggtcgct ctctgactcg gtcacgcgcg
     6001 tggtgttgac gattccggcc cctgccgatg ccaacagtga agaagtgacg gaaatcctgc
     6061 tcaccgcagc gcgtcgctgc tcgctggtaa tcgataaccc agcaccggaa gtcttcctgg
     6121 tggatctgca acaggggatt caaattttcg agctgcgtat ttacgccgct gagatgggtc
     6181 accgtatgcc actacgccat gagattcacc agctgattct ggcaggattc catgcccacg
     6241 gtattgatat gccattcccg cccttccaga tgcgtttgga aagcctcaac ggtaaacaaa
     6301 cggggagaac attaacttct gcgggcaaag gtcgtcaggc gggaagtttg taatgcatga
     6361 agctgccaaa tgatgtcaac gtggctcatt tagcagctta acatcatctt cagttatcac
     6421 tggccccgga gagtatcatc catacgcatt acttcctgca actcatcgcc ggagagacga
     6481 tgagttgcag gatttcatat gatcagtcga gagctaactc aggtttgttc gccatgcgac
     6541 tgcaatagtt ctggtaaatc gccatcgcta acagcgagaa gaagacaggg ccgccaatca
     6601 tccacaatgt gctgtcccag tctccagctt caaccacagg ttgaatgatg gtgaagacgt
     6661 tcgcaaatgt caccaccagt acgacaacca ctgttgcaat cattgccgac atatgcgttt
     6721 tgaaaatcac aaacggtctg tcgagatcct gacgtgcttt aaaaaatggg aaagccagcg
     6781 cgaggaacag gtaaggaagc gtcatagaca cgttcgccat cagcgtcagc ttgttaaaga
     6841 acgccgatgc ggtaccgcca ccaaacgaaa ccagcaggat gaagacagta accaacccac
     6901 actgcatcca catggcgata gacggcatcc ccatcgcatt caggcgcgtc atcggttccg
     6961 gccacaatgc tttcggcgtc ccctggatga tggctttcag cggtgaatag caaagtgtaa
     7021 agaacgcacc ggtataagcg aggaacatcg acagtccggt aatacgcgca aaccatacgc
     7081 ccagcgacaa tgacgcttca ggtgacaaat gcagtgcgtt acccagcgtc accccgaggc
     7141 tcttcatcag cacataggta atattgccga ggttaacgga accattactt aatacctgct
     7201 gccagtttgt gctgacgccc cataaaaata ttgccagcga ataaccgatt gaaataacaa
     7261 tagcggcaaa aacaatacct ttggcaaagt tcttttctgg attttccgtt ttatcaacca
     7321 gaccaccaac cgcttcaatt ccaccatagg caaaaatggc aaataccaca aacgatagca
     7381 ttgccagacc ggactgataa cctgggttcg gtgatgccag gaaattaata tcctgcgcga
     7441 aatgcccacc atttaataac aaaatagtaa tgcttactaa cagcaatact aaattcagac
     7501 acattactgc aataccgccc accgcagtaa tgcgggcaat tttattaatc ccttttgaag
     7561 caacgacggt gaccagaatc atccatgcca ctgccagcag accaaccacc tgcgtaggct
     7621 ccagtccggc aatatgccag tgctgggtca tgtcgctacc atagaggaat gttgagaacg
     7681 gtacccaaac tttcgcggag gtactcacca tccagatgat ataagaggaa aaccacataa
     7741 acgtaccaat gaaggcaaaa cgtggtccga cactattatt catccaggaa tagataccgc
     7801 cttcttcttt gcgataagca gctcccattt cagccatcat taaggcgaat ggaataaaga
     7861 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
     7921 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
     7981 tcatcttttt tatcgagtga ggcatgaaac catccttcat ttcagcgcgt gtataaaacg
     8041 cgcccactga actgcactgc ccaacattga gcgggcagcg taaaaaccaa tacagaattg
     8101 atacctggat gatatcaaat atccagtctt aaattgtggt ctttggcgga aaaatgaaaa
     8161 catatgctac agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
     8221 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
     8281 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
     8341 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
     8401 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgtcaatt catggaaacc
     8461 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
     8521 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
     8581 ttctttgcca atatttggct ctacgccaaa ggtaaacagc aattgtagaa gcgtgtcgcg
     8641 gtcttcttca gtatcggcaa cgttgctcaa atccagtttc gctgccactt cccgcagttg
     8701 cgttttgtcg gcagagagtg ggtcaatttc cagataacgc aagaaagctt gttgataaga
     8761 aaggctttct gctgccggac agtccagcac ctgctgcaag agatcgtcca cctcgttcat
     8821 caaccggtac atatcatagt gcggtcgata ccactcaagc atagtgaact cagggttgtg
     8881 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     8941 ggcaaccagc aggcgcttca tatggtattc cgggctggtc attaaccaga gattcatccc
     9001 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     9061 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     9121 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     9181 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     9241 tcgcaacgcg acggcgagac aaattttacg caggaataaa acaacggtgg gcagtgacta
     9301 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     9361 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     9421 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     9481 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     9541 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     9601 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     9661 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     9721 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     9781 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtgggggtg cccatggagc
     9841 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     9901 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     9961 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
    10021 ggtcatgttc gcggcctggt agcaatgaac atgatggaag gcacgctggt gcagatccgt
    10081 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
    10141 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
    10201 gacatggaat tcgttcagta tcacccaacc ggcctaccag gttccggtat cctgatgacc
    10261 gaaggctgcc gcggtgaagg tggtattctg gtcaacaaaa atggctaccg ttatctgcaa
    10321 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
    10381 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
    10441 tccacgccac gtggcgatgt ggtttacctc gacctgcgtc accttggcga gaaaaaactg
    10501 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
    10561 aaagaaccga ttccggtacg tccgaccgca cactacacca tgggcggtat cgaaaccgat
    10621 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
    10681 ctgcacggtg cgaaccgtct gggctccaac tcgctggcgg aactggtggt cttcggtcgt
    10741 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
    10801 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctggttaa ccaggatggc
    10861 ggcgaaaact gggctaagat ccgcgacgaa atgggcatgg ccatggaaga aggctgcggt
    10921 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
    10981 cgcttcaagc gcgtgcgcat caccgacact tccagcgtgt tcaacaccga cctgctctac
    11041 accattgagc tgggccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
    11101 cgtaaagagt cccgcggcgc gcaccagcgt ctggacgaag gttgcaccga gcgtgacgac
    11161 gtcaacttcc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
    11221 tacagcgacg tgaagattac tacgctgccg ccagctaaac gtgtttacgg tggcgaagcg
    11281 gatgcagccg ataaggcgga agcagccaat aagaaggaga aggcgaatgg ctgagatgaa
    11341 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
    11401 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
    11461 agacaacctg gcaccggacc tgagctaccg ctggtcctgc cgtatggcga tttgtggctc
    11521 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
    11581 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
    11641 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaacgc
    11701 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcga agtatcacca
    11761 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
    11821 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
    11881 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggcg tatggagctg
    11941 tactttcgtg ggctactgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
    12001 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
    12061 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
    12121 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
    12181 tgtggttcag cattgaactg attttcgggc tgtttgccct gaaaaatggc ccggaagcct
    12241 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
    12301 tggcggcagc cctgctgcac accaaaacct ggtttgagct ggcaccaaaa gcagccaata
    12361 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
    12421 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
    12481 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
    12541 ggtatgtgga gcgccatcat tgcgccggtg atgatcctgc tggttggtat tctgctgccg
    12601 ctagggctgt ttccgggtga tgcactgagc tacgagcgcg ttctggcgtt cgcgcagagc
    12661 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
    12721 cgtatgcacc acgcgatgca cgatctgaaa atccacgtac ctgcgggtaa atgggttttc
    12781 tacggtctgg ctgctatcct gacagttgtc acactgattg gtgtcgttac aatctaacgc
    12841 atcgccaatg taaatctggc ccgcctatgg cgggccgttt tgtatggaaa ccagacccta
    12901 tgttcaaaac gacgctctgc accttattaa ttaccgcctc ttgctccaca tttgccgcac
    12961 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
    13021 tccccggtat ggcggtagcg gtaatttatc agggtaaacc ttattacttt acctggggct
    13081 atgcgggcat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
    13141 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
    13201 taagcgatcc cacaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
    13261 cactattaca tctcgcaacc tacaccgctg gcggcctgcc attgcaggtg ccggatgagg
    13321 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
    13381 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
    13441 cgtctggttt gagttttgag caggcgatga aaactcgtgt cttccagcca ctcaaactca
    13501 atcatacatg gattaatgta ccgtccgcag acgaaaagaa ctacgcctgg ggatatcgcg
    13561 aaggtaaggc agtacatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
    13621 cgaccattga agatatggcc cgctgggtgc aaagcaattt aaaacccttt gatatcaatg
    13681 agaaaatact tcaacaaggg atacagctgg cacaatctcg ctactggcaa accggcgata
    13741 tgtatcaggg tctgggctgg gaaatgctgg actggccggt aaatcctgac atcatcatta
    13801 acggcagtga caataaaatt gcactggcag cacgccccgt aaaaccgatt acgcccccaa
    13861 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
    13921 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
    13981 ccaatccagc gagagttacc gccgcctggc agattctcaa cactctacag taaaatttca
    14041 tcgggtccga attttcggac cttttctccg cttttccttg ctgtcatcta cacttagaaa
    14101 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
    14161 tttctggtcg ttgcctgcag ttcccctacg ccgccgcgtg gcgtgaccgt agtgaataat
    14221 ttcgacgcta aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgcttt
    14281 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
    14341 gtcattaata aaggctataa ccctgacaga gaaatgtggc agcagagtga agggaaagcg
    14401 tactttaccg gagcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
    14461 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
    14521 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
    14581 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
    14641 cagcccggta gttaatgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
    14701 agactcgcca ggcgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
    14761 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctactgg taacgatttc
    14821 atcgcccagg caagtagcgc cagactgaca atcatcgccg tcacggtaat aacacttggc
    14881 gtcaaacgac taaagccgtg agtatatttc aggccaacgg cccataccac ttccagcaga
    14941 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaaattggg gccgtccccg
    15001 gtgaaaagac gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
    15061 ggtggcaggg gaatttcaac tattacctat gtaactatcg cagaaaaaac aaacggcacg
    15121 acacagctgt cgtaccgtta ttgcttattg cttattgctg cgctttcgtt gctgcgccag
    15181 aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg ttgcaggcag
    15241 ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc ttcaccataa
    15301 cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata atagacaaca
    15361 tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt agctggcagc
    15421 gcgggagatg gagttgccga ggtgtttgat atcttcgccg aaaccgcgag cggtgttaca
    15481 gcccgtaagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca tcatctctga
    15541 cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact tcacgcgaga
    15601 gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta caaacagcgg
    15661 aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac ctgcggtatc
    15721 acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg gcggagtaac
    15781 ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca gcaaccattt
    15841 agcgttgtcg ccaattgctt ttgcgtcagc agacagctgc tcgaaagttt cgttgttcat
    15901 gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga caacatcagc
    15961 gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg tcagcagacg
    16021 acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt cactcgcttc
    16081 aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat cgttgctata
    16141 gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac aaaatggcgc
    16201 atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt gccgatgttg
    16261 tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa aaactgttgg
    16321 ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt atcgatcgca
    16381 tggagaaagg caatccgaac gatccgcttt tgcgtcaggt acttacctcg caagatgagt
    16441 ttgtcgtcgc gcccggattc tccaccgacc cgctggaaga acagcatagc gtagtgcctg
    16501 gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc tgcgcggtaa
    16561 attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc aataagcgta
    16621 actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag atgattttct
    16681 ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc acacaactgg
    16741 aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt gtgatcccgg
    16801 cacgcatcac agatgcgctg gttgaacgct tttctcattc tacgctgcaa atcttgctgg
    16861 tgaatcacat caaccatgcc aatgaggtag atgaaacatt ccgtcaggcg atggctaagt
    16921 tgcgccgggt gggcgttact ttgctgaacc agagcgttct gttacgtggt gtgaacgata
    16981 acgcgcaaac gctggcaaac ctgagtaatg cgttgttcga tgcgggcgtg atgccgtatt
    17041 acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt gatgacgaag
    17101 cacggcagat tatgcgtgag ttgctgacac tggtgtcggg atatctggtg cctaaactgg
    17161 cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta cgccagcagt
    17221 aactgattaa acagggaagt ttaatctttg cccgttgcat aacctttaat tattcatata
    17281 cattgctgtt gttactcttt atataaccaa caccaataat taacctttct tttctgttta
    17341 tatagagtga gcacggctat ttatgttatt ttaacaac
//
LOCUS       DADSZD010000482         7631 bp    DNA     linear   BCT 05-APR-2021
DEFINITION  TPA_asm: Escherichia coli strain LGE2531
            SAMN08039590-rid11722853.guided.183, whole genome shotgun sequence.
ACCESSION   DADSZD010000482 DADSZD010000000
VERSION     DADSZD010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08039590
            Sequence Read Archive: SRR6362491
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAR-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAR-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000980120.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000980120.1
            Genome Coverage       :: 42x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/07/2021 04:07:24
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 4,709
            CDSs (total)                      :: 4,662
            Genes (coding)                    :: 4,558
            CDSs (with protein)               :: 4,558
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 39
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 53 of 104
            Pseudo Genes (incomplete)         :: 57 of 104
            Pseudo Genes (internal stop)      :: 21 of 104
            Pseudo Genes (multiple problems)  :: 23 of 104
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08039590-rid11722853.guided.183"
                     /strain="LGE2531"
                     /isolation_source="Soil"
                     /db_xref="taxon:562"
                     /country="USA: Minnesota, Buffalo River Watershed"
                     /lat_lon="46.8346 N 95.9211 W"
                     /collection_date="2015-08-24"
     gene            11..463
                     /locus_tag="J1Z28_004702"
     CDS             11..463
                     /locus_tag="J1Z28_004702"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBA7719428.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            641..1792
                     /locus_tag="J1Z28_004703"
     CDS             641..1792
                     /locus_tag="J1Z28_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBA7719429.1"
                     /translation="MEWYMGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQ
                     RVVGMPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEK
                     RLSDLDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALA
                     GLDAQHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARI
                     NRQLIALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGS
                     RGDYSWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICR
                     DKQLIFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1789..2403
                     /locus_tag="J1Z28_004704"
     CDS             1789..2403
                     /locus_tag="J1Z28_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBA7719430.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(2460..3917)
                     /gene="pepD"
                     /locus_tag="J1Z28_004705"
     CDS             complement(2460..3917)
                     /gene="pepD"
                     /locus_tag="J1Z28_004705"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008783788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBA7719431.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4178..4636
                     /gene="gpt"
                     /locus_tag="J1Z28_004706"
     CDS             4178..4636
                     /gene="gpt"
                     /locus_tag="J1Z28_004706"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBA7719432.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            4728..5972
                     /gene="frsA"
                     /locus_tag="J1Z28_004707"
     CDS             4728..5972
                     /gene="frsA"
                     /locus_tag="J1Z28_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBA7719433.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKVVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            6030..6431
                     /locus_tag="J1Z28_004708"
     CDS             6030..6431
                     /locus_tag="J1Z28_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBA7719434.1"
                     /translation="MTLPSGHPKSRLVKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAEVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(6470..7525)
                     /gene="phoE"
                     /locus_tag="J1Z28_004709"
     CDS             complement(6470..7525)
                     /gene="phoE"
                     /locus_tag="J1Z28_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBA7719435.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTSEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         1877 a         1855 c         1940 g         1959 t
ORIGIN      
        1 aatttatatt atgaataaca tacaaataag aaactatcag cctggtgatt ttcagcaact
       61 atgcgctatt ttccttcgag ctgttacgat gacggcgagt cagcactatt ctccacagca
      121 aattgccgcc tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg cgaaatcaca
      181 agtgcgggtt gcagttatta atgcacaacc ggttggtttt attacccgca ttggacatta
      241 tatcgatatg ttatttgttg aaccggaata cacccgacgc ggggttgcca gcgctttgtt
      301 aaaaccattg attaagtctg aatccgaact tacggtggac gccagcataa ccgcaaaacc
      361 cttttttgaa cgttatggtt ttcagacagt taagcagcag cgcgttgaat gccggggaac
      421 gtggtttact aatttttata tgcgatataa accgcaacat taaatccagc ttgcaatgaa
      481 aataacgccc gtctagtatg tgcgcgtttt cttattccac aaactgcaag gaggtaaatc
      541 atgacaaatc ctttatcaat gactctttgc agacctttcc aggattaatt cttttttctt
      601 gccctggatt cgtctgccat ttcctgattt ttattcataa atggaatggt acatgggcaa
      661 atatattcgt cccttatccg atgcggtatt taccatcgca tctgatgacc tgtggatcga
      721 gagtttagcg atccaacaat tacacaccac ggcaaattta cccaacatgc agcgcgttgt
      781 ggggatgcca gatttacatc ccggacgcgg ctacccgatt ggcgcagcgt tcttctctgt
      841 tggtcgtttt tacccggcac tggtcggcaa tgatatcggc tgcggcatgg cgctatggca
      901 aacagatatt ctcgctcgca aatacaacgc cgataagttt gaaaagcgat tatcagatct
      961 ggatgacgtt gctgaagaaa gctggctgga ggaaaacctg ccgtcagcat ttgcacagca
     1021 tccgtggcgc aactcgcttg gctccatcgg tggcggtaac cacttcgcag aactgcaaca
     1081 gattgatcaa attatcgacg cggaactgtt tgcactggct ggtctggatg cgcagcatct
     1141 gcaactgctg gttcatagcg gctcgagggg attggggcag tctattttac agcggcatat
     1201 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac gctatattgc
     1261 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatcg ctttgcgcat
     1321 aatgcaacaa gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
     1381 cgcgtgccaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
     1441 caacggtctg gtgattattc ccggttcacg cggcgattac tcctggctgg ttaaacctgt
     1501 tgcgaacgaa aaaacgctcc actcgctggc gcatggggcg gggcgtaaat gggggcgcac
     1561 cgagtgtaaa gggcgtctgg cagcgaaata cacggcgacg caactctcgc ggactgaact
     1621 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
     1681 taaatcggct gaaagcgtgg tgcaatgtct ggtgctggct gggttaatta ttcctgtcgc
     1741 gcgactgcgc ccggtgctga cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
     1801 caactctcct ctgctcaggg gccggaagaa tgttgtctcg cagtgagaaa agcactggac
     1861 aggctgatta aagaagctac ccgacaggac gtcgcggtaa cggtgctgga aacagaaacg
     1921 ggtcgctact ctgacacact gcgttcggcg ctgatttctc tggatggcga taacgcatgg
     1981 gctctaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
     2041 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
     2101 caatcggatg caatccgtta tgagacgctg cgttcgtcgg ggccgggcgg tcaacatgtc
     2161 aataaaaccg actcggcggt acgcgccacg catctggcat ccggtattag cgtgaaggtt
     2221 cagtcagagc gcagtcagca tgctaacaaa cggctggcgc gattactgat tgcctggaag
     2281 ctggaacaac agcaacagga aaatagcgcg gcgctgaaat cgcagcggcg aatgttccat
     2341 caccagattg aacgtggcaa cccgcgacga acgtttacag ggatggcttt tatcgaaggg
     2401 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     2461 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg gccaacgctt
     2521 tcgatatgaa cttgctcatc cggggagtgt ggaccggtga tggttggccc gatagacacc
     2581 atgtccattt ccggatacgg ttttttgaac aggccacatt cgaggcccgc gtggataatc
     2641 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcattacc
     2701 ggagagttag cgtccggctg ccagccagga tatgcgcctt ttgcttcggt tttcgcacct
     2761 gccagtttac ccagcgaatc cagcatgctc actacgtagt ctttaccgct gtcgatcaga
     2821 gaacggatca ggcagtgaat ttctacgtta ttgtcggtca tggtcaccac accgacgttc
     2881 agggaggttt cgaccacacc ttttgccacg tcggagttgc ggatcacacc gttcggggtg
     2941 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     3001 gcaacagagt ccagcagcaa tgccagattt ttctctttct ctgccagttc gtttttcagg
     3061 atctcctgat aggtatccac cagagatttc agggcgtcga ctttatcagc tgcgacagca
     3121 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcaata
     3181 agacgcaggt ccagttcttc tgcatgaccc gccaggaagc gcaccaacag tttgttggca
     3241 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt
     3301 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     3361 aagtcgatac ctcccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     3421 atcagaatat cagcctgcaa ccagttgctc tgtaagccaa aagcaccgtc cataccggct
     3481 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     3541 accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgtgct
     3601 ttaacccatt cgccatcaat ataaggctgg ataggatctt tagtgaagtc atgcacggtg
     3661 tcgttatttt tttgcggcac catatcgagg tgggcctgca agacgaccgg tttacgattt
     3721 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     3781 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     3841 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     3901 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     3961 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     4021 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     4081 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     4141 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     4201 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     4261 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     4321 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     4381 gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     4441 tgacctggta gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     4501 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgatt atgttgttga
     4561 tatcccgcaa aatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     4621 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     4681 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     4741 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     4801 gccgctttag tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     4861 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccccgtg
     4921 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     4981 tatacgatac ggtgattggc taccgcggcg gcaactggat ttatgagtgg gctacccagg
     5041 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagc ggtcgacact
     5101 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     5161 tggccgagca agcgcaggct ttgtcaaacc gcgcctatga agaggccgct cagcgtctgc
     5221 cgggcacgat gcgacagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     5281 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     5341 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcta
     5401 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
     5461 ccagcctgtt gcatcagcac gttttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
     5521 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatacc
     5581 ttgaatcgcc gcgtctgaaa gtggttgcct gtcttggtcc ggtagttcat accctgttga
     5641 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gccagtcgtt
     5701 tagggatgca tgatgcttcc gatgaagcgt tgcgagtgga gttgaatcgc tattcattaa
     5761 aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     5821 acgatccgtt cagcccggaa gaggactcac gcttaatcac gtcgtcatct gctgacggta
     5881 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggc cttcaggaaa
     5941 tcacagactg gatcgaaaaa cgtttgtgtt aaaaatttgc taaattttac caatttggta
     6001 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     6061 gattagtcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     6121 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     6181 gtgagttctg gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagtt
     6241 accagtttgg tctgttcgat aaagcaggcg actggacgag tgttcagata aaagatgccg
     6301 aagtggttga acgactggag cacaccctgc gtgaatttca cgagaagctg cgtgagctgc
     6361 tggcgacgct gaatctgaag ctggaaccgg cagatgattt tcgtgatgaa ccggtgaagt
     6421 taacggcgtg aatgaaatgt gccggatgca tcacatccgg caatatccat taaaactgat
     6481 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     6541 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     6601 caacatcgac ataattgacc agatcttcat caccgatgcc ctcaatatct ttccctttcg
     6661 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     6721 caaagttctg cgtcttattg gcaaagccgc cagagattgg cgtcatttta cgtgtttcag
     6781 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     6841 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gctggtgcga tcagagttgg
     6901 tataggctcc actgatggcg aaatcgctgc caccaaagtc atatgtcaat gacgtgccga
     6961 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     7021 ttaagttcag accatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     7081 tggcgcgttt ggtcataaag ttatcggtct gtgcggagga gtcgccacca aattccggga
     7141 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     7201 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc tgtgcagtat
     7261 cactctccgc tttattaccg gcaaactctg cttcccagcg accataaccc gtcagttgat
     7321 cgttaatttg tgtttcgcct ttaaaaccaa aacggatata actctggtcg ccatctttac
     7381 tgtcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     7441 cgtctttgtt atatatttct gcggcctgta cggatgcaga tgccacaatg cccatcacca
     7501 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     7561 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     7621 acagattaat g
//
